Sample records for amplicor roche

  1. Comparison of the Becton Dickinson strand displacement amplification and Cobas Amplicor Roche PCR for the detection of Chlamydia trachomatis: pooling versus individual tests

    DEFF Research Database (Denmark)

    Bang, D; Angelsø, Lene; Schirakow, Bente


    The objective of the study was to examine the influence of pooling Chlamydia trachomatis specimens. We compared Becton Dickinson ProbeTec strand displacement amplification (SDA) with Cobas Amplicor Roche (PCR). With PCR as the standard, SDA performed equally well in single-sample testing. For poo......The objective of the study was to examine the influence of pooling Chlamydia trachomatis specimens. We compared Becton Dickinson ProbeTec strand displacement amplification (SDA) with Cobas Amplicor Roche (PCR). With PCR as the standard, SDA performed equally well in single-sample testing...

  2. Evaluation of the NucliSens EasyQ v2.0 assay in comparison with the Roche Amplicor v1.5 and the Roche CAP/CTM HIV-1 Test v2.0 in quantification of C-clade HIV-1 in plasma.

    Directory of Open Access Journals (Sweden)

    Maximilian Muenchhoff

    Full Text Available Human immunodeficiency virus type 1 (HIV-1 genetic diversity poses a challenge to reliable viral load monitoring. Discrepancies between different testing platforms have been observed, especially for non-clade-B virus. Therefore we compare, in antiretroviral therapy (ART-naïve South African subjects predominantly infected with HIV-1 clade-C, three commercially available assays: the COBAS AmpliPrep/COBAS TaqMan HIV-1 Test version 2.0 by Roche (CAP/CTM v2.0, the BioMérieux NucliSens Version 2.0 Easy Q/Easy Mag (NucliSens v2.0 and the Roche COBAS Amplicor HIV-1 Monitor Test Version 1.5 (Amplicor v1.5. Strong linear correlation was observed and Bland-Altman analyses showed overall good agreement between the assays with mean viral load differences of 0.078 log cp/ml (NucliSens v2.0 - Amplicor v1.5, 0.260 log cp/ml (CAP/CTM v2.0 - Amplicor v1.5 and 0.164 log cp/ml (CAP/CTM v2.0 - NucliSens v2.0, indicating lower mean viral load results for the Amplicor v1.5 and higher mean readings for the CAP/CTM v2.0. Consistent with observations following previous comparisons of CAP/CTM v2.0 versus Amplicor v1.5, the CAP/CTM v2.0 assay detected low-level viremia (median 65 cp/ml in more than one-third of those in whom viremia had been undetectable (<20 cp/ml in assays using the NucliSens platform. These levels of viremia are of uncertain clinical significance but may be of importance in early detection of ART resistance in those on treatment. Overall the three assays showed good comparability of results but with consistent, albeit relatively small, discrepancies for HIV-1 clade-C samples, especially in the low-viremic range that should be taken into account when interpreting viral load data.

  3. Comparison of the Roche COBAS Amplicor Monitor, Roche COBAS Ampliprep/COBAS Taqman and Abbott RealTime Test assays for quantification of hepatitis C virus and HIV RNA. (United States)

    Wolff, Dietmar; Gerritzen, Andreas


    We have evaluated the performance of two newly developed automated real-time PCR assays, the COBAS Ampliprep/COBAS TaqMan (CAP/CTM) and the Abbott RealTime tests, in the quantification of human immunodeficiency virus (HIV) and hepatitis C virus (HCV) RNA. The widely used semi-automated COBAS Amplicor Monitor (CAM) assay served as the reference test. Several specimens were analyzed, including 102 plasma samples from HCV patients and 109 from HIV patients and 10 samples from negative donors, as well as Quality Control in Molecular Diagnostics (QCMD) and National Institute for Biological Standards and Controls (NIBSC) proficiency program panels. Good correlation was observed among the three assays, with correlation coefficients (R2) of 0.8 (CAM-CAP/CTM), 0.89 (CAM-RealTime) and 0.91 (CAP/CTM-RealTime) for HCV and 0.83 (CAM-RealTime), 0.85 (CAM-CAP/CTM) and 0.89 (CAP/CTM-RealTime) for HIV. The overall concordance for negative/positive results was 100% for HCV and 98% for HIV. All assays were equally able to quantify HCV genotypes 1, 3, 5 and HIV group M (subtypes A-H) and N from QCMD and NIBSC panels. In terms of workflow, the RealTime assay requires more hands-on-time than the CAP/CTM assay. The results indicate that real-time PCR assays can improve the efficiency of end-point PCR tests by better covering viral dynamic ranges and providing higher throughput and automation.

  4. Comparison of the Cobas 4800 Human Papillomavirus test against a combination of the Amplicor Human Papillomavirus and the Linear Array tests for detection of HPV types 16 and 18 in cervical samples. (United States)

    Martínez, Samuel Bernal; Palomares, José Carlos; Artura, Antonio; Parra, Manuel; Cabezas, Jose Luis; Romo, Jose Ma; Martín-Mazuelos, Estrella


    The greater prevalence of human papillomavirus (HPV) types 16 and 18 compared to the other high-risk HPV types of cervical cancer led to the development of clinical tests that detect both types separately from other genotypes. One method is the Roche Cobas 4800 HPV test, which is based on a real-time PCR. The aim of this study was to evaluate the performance of the Cobas 4800 HPV test for detecting genotypes 16 and 18 by comparing the results with those obtained in a combination of the Roche Amplicor HPV assay and the Roche Linear Array (LA) HPV genotyping assay. Excellent concordance was found between both methods (92.7%, kappa value=0.872). The Cobas 4800 HPV test could be used as a single test for identifying HPV types 16 and 18 directly from clinical specimens. Copyright © 2011 Elsevier B.V. All rights reserved.

  5. Meeting C.Roche

    CERN Multimedia



    Exposé de Christian Roche suite à un certain nombre d'évémements survenus depuis septembre 1985 concernant la restructuration de l'administration qui ont abouti à une décision prise par la direction

  6. Homage to Jean Roche

    CERN Multimedia


    It was with deep sadness that we learnt that Jean Roche had died. Jean was one of those quietly efficient and conscientious pillars of our community who beaver away unobtrusively for decades and make such a contribution to building CERN. An expert in thermal engineering, he left his mark on many buildings on the Meyrin and Prévessin sites. At the time of the LEP project at the beginning of the 1980s he was in charge of defining the principles of the ventilation systems for the new accelerator. Through his design studies and the calls for tenders that he launched, he developed these high-performance state-of-the-art installations which not only made LEP’s successes possible but also served as benchmarks for the fundamental principles underpinning the design of the LHC ventilation system. Jean always liked to surround himself with young people and during the years of his stewardship the design office was a splendid training ground. Despite his responsibilities, Jean was always keen t...

  7. COBAS AMPLICOR: fully automated RNA and DNA amplification and detection system for routine diagnostic PCR. (United States)

    DiDomenico, N; Link, H; Knobel, R; Caratsch, T; Weschler, W; Loewy, Z G; Rosenstraus, M


    The COBAS AMPLICOR system automates amplification and detection of target nucleic acids, making diagnostic PCR routine for a variety of infectious diseases. The system contains a single thermal cycler with two independently regulated heating/cooling blocks, an incubator, a magnetic particle washer, a pipettor, and a photometer. Amplified products are captured on oligonucleotide-coated paramagnetic microparticles and detected with use of an avidin-horseradish peroxidase (HRP) conjugate. Concentrated solutions of amplicon or HRP were pipetted without detectable carryover. Amplified DNA was detected with an intraassay CV of < 4.5%; the combined intraassay CV for amplification and detection was < 15%. No cross-reactivity was observed when three different target nucleic acids were amplified in a single reaction and detected with three target-specific capture probes. The initial COBAS AMPLICOR menu includes qualitative tests for diagnosing infections with Chlamydia trachomatis, Neisseria gonorrhoeae, Mycobacterium tuberculosis, and hepatitis C virus. All tests include an optional Internal Control to provide assurance that specimens are successfully amplified and detected.

  8. Comparison of COBAS AMPLICOR Neissefia gonorrhoeae PCR, including confirmation with N-gonorrhoeae-specific 16S rRNA PCR, with traditional culture

    NARCIS (Netherlands)

    Luijt, DS; Bos, PAJ; van Zwet, AA; Vader, PCV; Schirm, J

    A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased

  9. Linnugripp tõi ravimifirmale Roche magusa kasumi / Erik Aru

    Index Scriptorium Estoniae

    Aru, Erik


    Šveitsi ravimitootja Roche'i viiruseravimi Tamiflu müük on kasvanud. Roche kavatseb järgmiseks suveks suurendada toodangut võrreldes 2004. aastaga kaheksa korda, kuid seda ei peeta piisavaks. ÜRO ja WHO nõuavad, et firma loobuks patentidest või annaks koopiaravimite valmistajatele tootmise litsentsi. Tabel: F. Hoffmann-La Roche. Lisa: Linnugripi vaktsiini võib loota kuue kuu pärast

  10. Ice cream and orbifold Riemann-Roch (United States)

    Buckley, Anita; Reid, Miles; Zhou, Shengtian


    We give an orbifold Riemann-Roch formula in closed form for the Hilbert series of a quasismooth polarized n-fold (X,D), under the assumption that X is projectively Gorenstein with only isolated orbifold points. Our formula is a sum of parts each of which is integral and Gorenstein symmetric of the same canonical weight; the orbifold parts are called ice cream functions. This form of the Hilbert series is particularly useful for computer algebra, and we illustrate it on examples of {K3} surfaces and Calabi-Yau 3-folds. These results apply also with higher dimensional orbifold strata (see [1] and [2]), although the precise statements are considerably trickier. We expect to return to this in future publications.

  11. An integral Riemann-Roch theorem for surface bundles

    DEFF Research Database (Denmark)

    Madsen, Ib Henning


    This paper is a response to a conjecture by T. Akita about an integral Riemann–Roch theorem for surface bundles.......This paper is a response to a conjecture by T. Akita about an integral Riemann–Roch theorem for surface bundles....

  12. 75 FR 71135 - Hoffmann-La Roche Inc.; Withdrawal of Approval of a New Drug Application (United States)


    ... HUMAN SERVICES Food and Drug Administration Hoffmann-La Roche Inc.; Withdrawal of Approval of a New Drug... held by Hoffmann-La Roche Inc., 340 Kingsland St., Nutley, NJ 07110- 1199. Hoffmann-La Roche Inc... INFORMATION: Hoffmann-La Roche Inc. has requested that FDA withdraw approval of NDA 18-662, ACCUTANE...

  13. Comparison of two commercial assays for detection of human papillomavirus (HPV) in cervical scrape specimens: validation of the Roche AMPLICOR HPV test as a means to screen for HPV genotypes associated with a higher risk of cervical disorders.

    NARCIS (Netherlands)

    Ham, M.A.P.C. van; Bakkers, J.M.J.E.; Harbers, G.; Quint, W.G.V.; Massuger, L.F.A.G.; Melchers, W.J.G.


    Certain high-risk (HR) human papillomavirus (HPV) types are a necessary cause for the development of cervical disorders. Women with persistent HR HPV infections have an increased risk of developing high-grade cervical lesions, compared with those who have no or low-risk HPV infections. Therefore,

  14. Transference of CALIPER pediatric reference intervals to biochemical assays on the Roche cobas 6000 and the Roche Modular P. (United States)

    Higgins, Victoria; Chan, Man Khun; Nieuwesteeg, Michelle; Hoffman, Barry R; Bromberg, Irvin L; Gornall, Doug; Randell, Edward; Adeli, Khosrow


    The Canadian Laboratory Initiative on Pediatric Reference Intervals (CALIPER) has recently established pediatric age- and sex-specific reference intervals for over 85 biochemical markers on the Abbott Architect system. Previously, CALIPER reference intervals for several biochemical markers were successfully transferred from Abbott assays to Roche, Beckman, Ortho, and Siemens assays. This study further broadens the CALIPER database by performing transference and verification for 52 biochemical assays on the Roche cobas 6000 and the Roche Modular P. Using CLSI C28-A3 and EP9-A2 guidelines, transference of the CALIPER reference intervals was attempted for 16 assays on the Roche cobas 6000 and 36 on the Modular P. Calculated reference intervals were further verified using 100 healthy CALIPER samples. Most assays showed strong correlation between assay systems and were transferable from Abbott to the Roche cobas 6000 (81%) and the Modular P (86%). Bicarbonate and magnesium were not transferable on either system and calcium and prealbumin were not transferable to the Modular P. Of the transferable analytes, 62% and 61% were verified on the cobas 6000 and the Modular P, respectively. This study extends the utility of the CALIPER database to two additional analytical systems, which facilitates the broad application of CALIPER reference intervals at pediatric centers utilizing Roche biochemical assays. Transference studies across different analytical platforms can later be collectively analyzed in an attempt to develop common reference intervals across all clinical chemistry instruments to harmonize laboratory test interpretation in diagnosis and monitoring of pediatric disease. Copyright © 2015 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.


    Energy Technology Data Exchange (ETDEWEB)

    Salmon, Julien; Canup, Robin M., E-mail:, E-mail: [Department of Space Studies, Southwest Research Institute, 1050 Walnut Street, Suite 300, Boulder, CO 80302 (United States)


    We use a hybrid numerical approach to simulate the formation of the Moon from an impact-generated disk, consisting of a fluid model for the disk inside the Roche limit and an N-body code to describe accretion outside the Roche limit. As the inner disk spreads due to a thermally regulated viscosity, material is delivered across the Roche limit and accretes into moonlets that are added to the N-body simulation. Contrary to an accretion timescale of a few months obtained with prior pure N-body codes, here the final stage of the Moon's growth is controlled by the slow spreading of the inner disk, resulting in a total lunar accretion timescale of {approx}10{sup 2} years. It has been proposed that the inner disk may compositionally equilibrate with the Earth through diffusive mixing, which offers a potential explanation for the identical oxygen isotope compositions of the Earth and Moon. However, the mass fraction of the final Moon that is derived from the inner disk is limited by resonant torques between the disk and exterior growing moons. For initial disks containing <2.5 lunar masses (M{sub Last-Quarter-Moon }), we find that a final Moon with mass > 0.8 M{sub Last-Quarter-Moon} contains {<=}60% material derived from the inner disk, with this material preferentially delivered to the Moon at the end of its accretion.

  16. Submaximal Riemann-Roch expected curves and symplectic packing.

    Directory of Open Access Journals (Sweden)

    Wioletta Syzdek


    Full Text Available We study Riemann-Roch expected curves on $mathbb{P}^1 imes mathbb{P}^1$ in the context of the Nagata-Biran conjecture. This conjecture predicts that for sufficiently large number of points multiple points Seshadri constants of an ample line bundle on algebraic surface are maximal. Biran gives an effective lower bound $N_0$. We construct examples verifying to the effect that the assertions of the Nagata-Biran conjecture can not hold for small number of points. We discuss cases where our construction fails. We observe also that there exists a strong relation between Riemann-Roch expected curves on $mathbb{P}^1 imes mathbb{P}^1$ and the symplectic packing problem. Biran relates the packing problem to the existence of solutions of certain Diophantine equations. We construct such solutions for any ample line bundle on $mathbb{P}^1 imes mathbb{P}^1$ and a relatively smallnumber of points. The solutions geometrically correspond to Riemann-Roch expected curves. Finally we discuss in how far the Biran number $N_0$ is optimal in the case of mathbb{P}^1 imes mathbb{P}^1. In fact we conjecture that it can be replaced by a lower number and we provide evidence justifying this conjecture.

  17. Abbott RealTime hepatitis C virus (HCV) and Roche Cobas AmpliPrep/Cobas TaqMan HCV assays for prediction of sustained virological response to pegylated interferon and ribavirin in chronic hepatitis C patients. (United States)

    Matsuura, Kentaro; Tanaka, Yasuhito; Hasegawa, Izumi; Ohno, Tomoyoshi; Tokuda, Hiroshi; Kurbanov, Fuat; Sugauchi, Fuminaka; Nojiri, Shunsuke; Joh, Takashi; Mizokami, Masashi


    Two commercial real-time PCR assays are currently available for sensitive hepatitis C virus (HCV) RNA quantification: the Abbott RealTime HCV assay (ART) and Roche Cobas AmpliPrep/Cobas TaqMan HCV assay (CAP/CTM). We assessed whether the two real-time PCR assays were more effective than Roche Cobas Amplicor HCV Monitor test, v.2.0 (CAM) for prediction of the sustained virological response (SVR) to pegylated interferon (PEG-IFN) plus ribavirin (RBV) in chronic hepatitis C. Sixty patients chronically infected with HCV genotype 1b (37 males and 23 females, 53 +/- 12 years of age) were treated with PEG-IFNalpha2b plus RBV for 48 weeks. Stored specimens at nine time points for each patient (at baseline, on treatment, and 24 weeks after treatment) were tested by the two real-time PCR assays and CAM. Twenty-six (43.3%) patients reached SVR. The positive predictive values (PPVs) for SVR of undetectable HCV RNA at week 12 by CAM, ART, and CAP/CTM were 74.3%, 88.0%, and 95.2%, respectively. An undetectable HCV RNA level by CAM, ART, and CAP/CTM correctly predicted SVR at week 4 in 100%, 100%, and 100% of patients, at weeks 5 to 8 in 91.7%, 100%, and 100% of patients, at weeks 9 to 12 in 55.6%, 75%, and 87.5% of patients, and at weeks 13 to 24 in 0%, 26.7%, and 40% of patients, respectively. Of 16 patients who relapsed after treatment, HCV RNA was detectable in 2 patients at the end of treatment by CAP/CTM but undetectable by ART and CAM. HCV RNA tests using ART and CAP/CTM are considered to be more effective at predicting SVR than CAM, and the PPV for SVR was slightly higher in CAP/CTM than in ART.

  18. Mécanique des sols et des roches

    CERN Document Server

    Vullier, Laurent; Zhao, Jian


    La mécanique des sols et la mécanique des roches sont des disciplines généralement traitées séparément dans la littérature. Pour la première fois, un traité réunit ces deux spécialités, en intégrant également les connaissances en lien avec les écoulements souterrains et les transferts thermiques. A la fois théorique et pratique, cet ouvrage propose tout d'abord une description détaillée de la nature et de la composition des sols et des roches, puis s'attache à la modélisation de problèmes aux conditions limites et présente les essais permettant de caractériser les sols et les roches, tant d'un point de vue mécanique qu'hydraulique et thermique. La problématique des sols non saturés et des écoulements multiphasiques est également abordée. Une attention particulière est portée aux lois de comportement mécanique et à la détermination de leurs paramètres par des essais in situ et en laboratoire, et l'ouvrage offre également une présentation détaillée des systèmes de classi...

  19. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.

    We construct linear network codes utilizing algebraic curves over finite fields and certain associated Riemann-Roch spaces and present methods to obtain their parameters. In particular we treat the Hermitian curve and the curves associated with the Suzuki and Ree groups all having the maximal...... number of points for curves of their respective genera. Linear network coding transmits information in terms of a basis of a vector space and the information is received as a basis of a possibly altered vector space. Ralf Koetter and Frank R. Kschischang %\\cite{DBLP:journals/tit/KoetterK08} introduced...... in the above metric making them suitable for linear network coding....

  20. `Similar' coordinate systems and the Roche geometry: application (United States)

    Roman, Rodica


    A new equivalence relation, named relation of `similarity' is defined and applied in the restricted three-body problem. Using this relation, a new class of trajectories (named `similar' trajectories) are obtained; they have the theoretical role to give us new details in the restricted three-body problem. The `similar' coordinate systems allow us in addition to obtain a unitary and an elegant demonstration of some analytical relations in the Roche geometry. As an example, some analytical relations published by Seidov (in Astrophys. J. 603:283, 2004) are demonstrated.

  1. Small-scale hydro-electric scheme in Roches, Switzerland

    International Nuclear Information System (INIS)

    Hausmann, M.


    The small hydropower plant (SHPP) project 'PCH Roches' is planned to be built in Roches, a small village located in the Jura region of the canton of Bern. The goal of this undertaking is to reactivate a site with hydro potential of the river Birs on a section that was already well exploited by some ancient SHPP (since 1953 or probably even earlier). Several modifications were performed on this plant over the years; not all contributed positively on its production figures. Following a transfer of the water rights in 1987, claims became loud against the owner to perform maintenance on the river banks and to modify the weir such that a free passage for the fishes will be granted. Those issues contributed to inflate a conflict which ended in the repeal of the water rights and a total plant shut down in 2001. The new project plans to reuse the existing pen stock and to carry out some refurbishing activities to grant security and better performance. The weir and the intake structure are also redesigned such that the water storage can be somewhat raised for normal operation; a weir gate will however allow to better discharge the river in flood situations. A new turbine house will be erected at the current location where the tail water pen stock rejoins the river. Hence, the existing old turbine house will be obsolete and the upper and lower pen stock sections are to be linked with each other. Limited by the existing pen stock size, the new plant is expected to produce some 50 kW power and 362,000 kWh/year. This represents an increase in energy generation of around 200% compared with the ancient SHPP. Gross head is 5.38 m, net head 4.12 m with a turbine flow of at least 1.6 m 3 /s. The expected energy sales price applicable to this project reaches about 0.17 CHF/kWh as per the current tariffs set by the grid owner. This project calls for the building of a state-of-the-art SHPP. It will avoid the total dismantling of the existing works to take place and allow the small

  2. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.

    a metric on the set of vector spaces and showed that a minimal distance decoder for this metric achieves correct decoding if the dimension of the intersection of the transmitted and received vector space is sufficiently large. The vector spaces in our construction have minimal distance bounded from below......We construct linear network codes utilizing algebraic curves over finite fields and certain associated Riemann-Roch spaces and present methods to obtain their parameters. In particular we treat the Hermitian curve and the curves associated with the Suzuki and Ree groups all having the maximal...... number of points for curves of their respective genera. Linear network coding transmits information in terms of a basis of a vector space and the information is received as a basis of a possibly altered vector space. Ralf Koetter and Frank R. Kschischang %\\cite{DBLP:journals/tit/KoetterK08} introduced...

  3. The Roche Immunoturbidimetric Albumin Method on Cobas c 501 Gives Higher Values Than the Abbott and Roche BCP Methods When Analyzing Patient Plasma Samples. (United States)

    Helmersson-Karlqvist, Johanna; Flodin, Mats; Havelka, Aleksandra Mandic; Xu, Xiao Yan; Larsson, Anders


    Serum/plasma albumin is an important and widely used laboratory marker and it is important that we measure albumin correctly without bias. We had indications that the immunoturbidimetric method on Cobas c 501 and the bromocresol purple (BCP) method on Architect 16000 differed, so we decided to study these methods more closely. A total of 1,951 patient requests with albumin measured with both the Architect BCP and Cobas immunoturbidimetric methods were extracted from the laboratory system. A comparison with fresh plasma samples was also performed that included immunoturbidimetric and BCP methods on Cobas c 501 and analysis of the international protein calibrator ERM-DA470k/IFCC. The median difference between the Abbott BCP and Roche immunoturbidimetric methods was 3.3 g/l and the Roche method overestimated ERM-DA470k/IFCC by 2.2 g/l. The Roche immunoturbidimetric method gave higher values than the Roche BCP method: y = 1.111x - 0.739, R² = 0.971. The Roche immunoturbidimetric albumin method gives clearly higher values than the Abbott and Roche BCP methods when analyzing fresh patient samples. The differences between the two methods were similar at normal and low albumin levels. © 2016 Wiley Periodicals, Inc.

  4. Small-scale hydro-electric scheme in Roches, Switzerland; Projet de concession. Petite centrale hydroelectrique de Roches

    Energy Technology Data Exchange (ETDEWEB)

    Hausmann, M.


    The small hydropower plant (SHPP) project 'PCH Roches' is planned to be built in Roches, a small village located in the Jura region of the canton of Bern. The goal of this undertaking is to reactivate a site with hydro potential of the river Birs on a section that was already well exploited by some ancient SHPP (since 1953 or probably even earlier). Several modifications were performed on this plant over the years; not all contributed positively on its production figures. Following a transfer of the water rights in 1987, claims became loud against the owner to perform maintenance on the river banks and to modify the weir such that a free passage for the fishes will be granted. Those issues contributed to inflate a conflict which ended in the repeal of the water rights and a total plant shut down in 2001. The new project plans to reuse the existing pen stock and to carry out some refurbishing activities to grant security and better performance. The weir and the intake structure are also redesigned such that the water storage can be somewhat raised for normal operation; a weir gate will however allow to better discharge the river in flood situations. A new turbine house will be erected at the current location where the tail water pen stock rejoins the river. Hence, the existing old turbine house will be obsolete and the upper and lower pen stock sections are to be linked with each other. Limited by the existing pen stock size, the new plant is expected to produce some 50 kW power and 362,000 kWh/year. This represents an increase in energy generation of around 200% compared with the ancient SHPP. Gross head is 5.38 m, net head 4.12 m with a turbine flow of at least 1.6 m{sup 3}/s. The expected energy sales price applicable to this project reaches about 0.17 CHF/kWh as per the current tariffs set by the grid owner. This project calls for the building of a state-of-the-art SHPP. It will avoid the total dismantling of the existing works to take place and allow

  5. Transcript of presentation of Distinguished Alumni Award to James Roche and Thomas White, 2001


    Elster, Richard S.


    Transcript of the words of Provost Richard S. Elster from the September 2001 Distinguished Alumni Award recognition of Secretary of the Air Force the Honorable James Roche and Secretary of the Army the Honorable Thomas White.

  6. Disainmööbel Roche Bobois, kokteil sarmist ja romantikast / Reet Krause

    Index Scriptorium Estoniae

    Krause, Reet, 1967-


    Luksuslikku kodumööblit tootvast prantsuse firmast. Firma disainerid itaallased Paola Navone ja Massimo Iosa Ghini, Vladimir Kagan, Hans Hopper ja prantslased Jean Claude Magirard ja Sylvain Joly. Philippe Roche ja disainer Hans Hopperi kommentaar

  7. Evaluation of Five New Liquid Stable Applications on the Roche Cobas Integra


    Lippi, Giuseppe; Brentegani, Claudio; Mazzi, Clara; Recchi, Cristina; Ruzzente, Orazio; Guidi, Giancesare


    In the present study the analytical performances of five new liquid applications on the Roche Cobas Integra were evaluated: urea and high density lipoprotein (HDL) cholesterol in serum and glucose, creatinine and inorganic phosphorus in urine.

  8. Unstable Roche-Lobe Overflow of Gaseous Planets (United States)

    Jackson, Brian

    The discoveries of more than 100 roughly Earth-sized bodies with orbital periods less than 1 day, ultra-short-period planets or candidates (USPs), have challenged planet formation theories, and evidence suggests USPs may be the remnants of gaseous planets that shed their atmospheres. Indeed, many hot Jupiters are near Roche-Lobe overflow (RLO), and tidal decay can push them the rest of the way in. Recent work has shown stable RLO (atmospheres lost via a steady outflow and thin accretion disk) probably cannot produce USPs on its own but suggested unstable RLO (atmospheres quickly shed on dynamical timescales) may. In fact, stable RLO may drive overflowing hot Jupiters into unstable RLO, and by analogy with the common-envelope binaries, the core that remains can drive off the gaseous envelope at the cost of its orbital energy. Wellestablished mass-radius relations for gaseous planets, coupled to simple energy and angular momentum considerations, provide a connection between the observed masses and periods for USPs and their putative progenitor gaseous planets, with few free parameters. We propose to investigate the hypothesis that USPs originate through tidal decay and a combination of stable and unstable Roche-lobe overflow of short-period gaseous planets through the following studies: -We will explore the planetary masses, orbital periods, etc. that produce unstable RLO using the Modules for Experiments in Stellar Astrophysics (MESA) suite. -We will relate the observed periods and masses of USPs to their putative progenitor masses and periods to see whether they are consistent with the unstable RLO hypothesis. This proposal is directly relevant to the Exoplanets Research Program since it seeks to "understand the ... physical processes of exoplanets" and "improve understanding of [their] origins" through "theoretical studies ... and modeling'". We also expect that it will have broad impacts on a variety of astrophysical topics: -Ultra-short period planets could


    International Nuclear Information System (INIS)

    Tiscareno, Matthew S.; Hedman, Matthew M.; Burns, Joseph A.; Castillo-Rogez, Julie


    We consider the Roche critical density (ρ Roche ), the minimum density of an orbiting object that, at a given distance from its planet, is able to hold itself together by self-gravity. It is directly related to the more familiar ''Roche limit,'' the distance from a planet at which a strengthless orbiting object of given density is pulled apart by tides. The presence of a substantial ring requires that transient clumps have an internal density less than ρ Roche . Conversely, in the presence of abundant material for accretion, an orbiting object with density greater than ρ Roche will grow. Comparing the ρ Roche values at which the Saturn and Uranus systems transition rapidly from disruption-dominated (rings) to accretion-dominated (moons), we infer that the material composing Uranus' rings is likely more rocky, as well as less porous, than that composing Saturn's rings. From the high values of ρ Roche at the innermost ring moons of Jupiter and Neptune, we infer that those moons may be composed of denser material than expected, or more likely that they are interlopers that formed farther from their planets and have since migrated inward, now being held together by internal material strength. Finally, the ''Portia group'' of eight closely packed Uranian moons has an overall surface density similar to that of Saturn's A ring. Thus, it can be seen as an accretion-dominated ring system, of similar character to the standard ring systems except that its material has a characteristic density greater than the local ρ Roche .


    Energy Technology Data Exchange (ETDEWEB)

    Tiscareno, Matthew S.; Hedman, Matthew M. [Center for Radiophysics and Space Research, Cornell University, Ithaca, NY 14853 (United States); Burns, Joseph A. [Department of Astronomy, Cornell University, Ithaca, NY 14853 (United States); Castillo-Rogez, Julie [Jet Propulsion Laboratory, Pasadena, CA 91109 (United States)


    We consider the Roche critical density ({rho}{sub Roche}), the minimum density of an orbiting object that, at a given distance from its planet, is able to hold itself together by self-gravity. It is directly related to the more familiar ''Roche limit,'' the distance from a planet at which a strengthless orbiting object of given density is pulled apart by tides. The presence of a substantial ring requires that transient clumps have an internal density less than {rho}{sub Roche}. Conversely, in the presence of abundant material for accretion, an orbiting object with density greater than {rho}{sub Roche} will grow. Comparing the {rho}{sub Roche} values at which the Saturn and Uranus systems transition rapidly from disruption-dominated (rings) to accretion-dominated (moons), we infer that the material composing Uranus' rings is likely more rocky, as well as less porous, than that composing Saturn's rings. From the high values of {rho}{sub Roche} at the innermost ring moons of Jupiter and Neptune, we infer that those moons may be composed of denser material than expected, or more likely that they are interlopers that formed farther from their planets and have since migrated inward, now being held together by internal material strength. Finally, the ''Portia group'' of eight closely packed Uranian moons has an overall surface density similar to that of Saturn's A ring. Thus, it can be seen as an accretion-dominated ring system, of similar character to the standard ring systems except that its material has a characteristic density greater than the local {rho}{sub Roche}.

  11. On the Use of Roche Equipotentials in Analysing the Problems of ...

    Indian Academy of Sciences (India)

    Abstract. Kopal (Adv. Astron. Astrophys., 9, 1, 1972) introduced the concept of Roche equipotentials to analyse the effects of rotational and tidal distortions in case of stars in binary systems. In this approach a mathematical expression for the potential of a star in a binary system is obtained by approximating its inner structure ...

  12. On the Use of Roche Equipotentials in Analysing the Problems of ...

    Indian Academy of Sciences (India)

    obtained by approximating its inner structure with Roche model. This expression for the potential has been used in subsequent analysis by var- ious authors to analyse the problems of structures and oscillations of synchronous and nonsynchronus binary stars as well as single rotating stars. Occasionally, doubts have been ...

  13. Performance of the new Bayer VERSANT HCV RNA 3.0 assay for quantitation of hepatitis C virus RNA in plasma and serum: Conversion to international units and comparison with the Roche COBAS amplicor HCV monitor, version 2.0, assay

    NARCIS (Netherlands)

    Beld, Marcel; Sentjens, Roel; Rebers, Sjoerd; Weegink, Christine; Weel, Jan; Sol, Cees; Boom, René


    We have evaluated the VERSANT HCV RNA 3.0. Assay (HCV 3.0 bDNA assay) (Bayer Diagnostics, Berkeley, Calif.), which is an improved signal amplification procedure for the HCV 2.0 bDNA assay for the quantitation of hepatitis C virus (HCV) RNA in serum or plasma of HCV-infected individuals. The HCV 3.0

  14. Life near the Roche limit - Behavior of ejecta from satellites close to planets (United States)

    Dobrovolskis, A. R.; Burns, J. A.


    A study of the dynamics of nearby debris from impact craters was made to explain the distinctive features seen on Phobos, Deimis, and Amalthea. The planetary tides and satellite rotation were considered, and the usual pseudo-energy (Jacobi) integral was numerically calculated in the framework of a restricted body problem where satellites are modelled as triaxial ellipsoids rather than point masses. Iso-contours of this integral show that Deimos and Amalthea are entirely closed by Roche lobes, and the surfaces of their model ellipsoids lie nearly along equipotentials. Presently, the surface of Phobos overflows its Roche lobe, except for regions within a few km of the sub-Mars and anti-Mars points. The behavior of crater ejecta from the satellites of Mars were also examined by numerical integration of trajectories for particles leaving their surfaces in the equatorial plane.

  15. A new primate assemblage from La Verrerie de Roches (Middle Eocene, Switzerland). (United States)

    Minwer-Barakat, Raef; Marigó, Judit; Becker, Damien; Costeur, Loïc


    Primates reached a great abundance and diversity during the Eocene, favored by warm temperatures and by the development of dense forests throughout the Northern Hemisphere. Here we describe new primate material from La Verrerie de Roches, a Middle Eocene karstic infill situated in the Jura Region (Switzerland), consisting of more than 80 dental remains. The primate assemblage from La Verrerie de Roches includes five different taxa. The best represented primate is Necrolemur aff. anadoni, similar in size and overall morphology to Necrolemur anadoni but resembling in some features the younger species Necrolemur antiquus. Microchoerines are also represented by two species of Pseudoloris, P. pyrenaicus and Pseudoloris parvulus, constituting the unique joint record of these two species known up to now. Remains of Adapiformes are limited to one isolated tooth of a large anchomomyin and another tooth belonging to the small adapine Microadapis cf. sciureus. The studied primate association allows assigning La Verrerie de Roches to the Robiacian Land Mammal Age. More specifically, this site can be confidently situated between the MP15 and MP16 reference levels, although the primate assemblage probably indicates some degree of temporal mixing. This is the first record of P. pyrenaicus and a form closely related to N. anadoni out of the Iberian Peninsula. The identification of these microchoerines in Switzerland gives further support to the connection of NE Spain and Central Europe during the Middle Eocene. Copyright © 2017 Elsevier Ltd. All rights reserved.


    Energy Technology Data Exchange (ETDEWEB)

    Valsecchi, Francesca; Rasio, Frederic A. [Center for Interdisciplinary Exploration and Research in Astrophysics (CIERA), and Northwestern University, Department of Physics and Astronomy, Evanston, IL 60208 (United States); Rappaport, Saul [Department of Physics, and Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Marchant, Pablo [Argelander-Institut für Astronomie, Universität Bonn, Auf dem Hgel 71, D-53121 Bonn (Germany); Rogers, Leslie A., E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Department of Astronomy and Department of Geophysics and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 (United States)


    Many exoplanets have now been detected in orbits with ultra-short periods very close to the Roche limit. Building upon our previous work, we study the possibility that mass loss through Roche lobe overflow (RLO) may affect the evolution of these planets, and could possibly transform a hot Jupiter into a lower-mass planet (hot Neptune or super-Earth). We focus here on systems in which the mass loss occurs slowly (“stable mass transfer” in the language of binary star evolution) and we compute their evolution in detail with the binary evolution code Modules for Experiments in Stellar Astrophysics. We include the effects of tides, RLO, irradiation, and photo-evaporation (PE) of the planet, as well as the stellar wind and magnetic braking. Our calculations all start with a hot Jupiter close to its Roche limit, in orbit around a Sun-like star. The initial orbital decay and onset of RLO are driven by tidal dissipation in the star. We confirm that such a system can indeed evolve to produce lower-mass planets in orbits of a few days. The RLO phase eventually ends and, depending on the details of the mass transfer and on the planetary core mass, the orbital period can remain around a few days for several Gyr. The remnant planets have rocky cores and some amount of envelope material, which is slowly removed via PE at a nearly constant orbital period; these have properties resembling many of the observed super-Earths and sub-Neptunes. For these remnant planets, we also predict an anti-correlation between mass and orbital period; very low-mass planets (M{sub pl} ≲ 5 M{sub ⊕}) in ultra-short periods (P{sub orb} < 1 day) cannot be produced through this type of evolution.

  17. Analytical validation of the Roche 25-OH Vitamin D Total assay

    DEFF Research Database (Denmark)

    Knudsen, Cindy Soendersoe; Nexo, Ebba; Højskov, Carsten Schriver


    Background: Vitamin D deficiency is considered a major health issue and therefore there is a need for reliable routine tests for measurement of the vitamin in blood samples. Here we present a validation of the recently released Roche 25-OH Vitamin D Total assay (Vitamin D Total). Methods: We...... evaluated the 25-OH Vitamin D Total assay in comparison to our in-house liquid chromatography tandem mass spectrometry (LC-MS/MS) method [194 patient samples without 25-hydroxy vitamin D2 (25OHD2) and 23 patient samples containing 25OHD2]. Results: At concentrations of 34 and 56 nmol/L within-run CVs were 4......) showed Vitamin D Total nmol/L=1.07×(LC-MS/MS) nmol/L+4.7 nmol/L, whereas comparison of 25OHD2 using 23 patient samples showed Vitamin D Total nmol/L=0.55×(LC-MS/MS) nmol/L–2.38 nmol/L (Demings regression). Conclusions: The Roche Vitamin D Total assay is judged suitable for measurement of 25OHD in serum...


    Bencivenga, Alessandro; Di Luigi, Gianluca


    Ex voto are devotional objects, widely used in Italy as a form of prayer or wish, or as thanksgiving for a grace received. In a broader historical perspective, votive offerings are hung in Greek, Etruscan, Roman shrines and later in churches or to show gratitude for miracles obtained, healing from illness or infirmity, or as a simple supplication and prayer. The ex voto here presented seem to be unique in the world: made of majolica, they come from the church of St. Roch in Castelli (Abruzzo). They depict breast benign and malignant lesions (mastitis, abscess, fat necrosis, inflammatory cancer, infiltrating carcinoma) in three dimensions, performing an accurate and personalized portrait of breast diseases (in some cases with the depicted name of the sick woman), showing fine details of each disease, and demonstrating accurate knowledge of the female anatomy and pathology.

  19. Quantitative automated human chorionic gonadotropin measurement in urine using the Modular Analytics E170 module (Roche). (United States)

    Ajubi, Nasser E; Nijholt, Nine; Wolthuis, Albert


    Ongoing demands on laboratory performance require optimization of processes. An obvious way to achieve this is to reduce manual labor in favor of automated methods. We describe the validation of an automated quantitative urine human chorionic gonadotropin (hCG) analysis on the Roche Modular E170 analyzer to replace the manual qualitative pregnancy test in urine. At urine hCG concentrations of 476, 45 and 11 U/L, we found inter-assay variation of 4.3%, 4.3% and 6.8% and average intra-assay variation of 3.0%, 2.6% and 3.0%, respectively. The analytical detection limit was 0.7 U/L. We did not detect any loss (due to degradation or adsorption) during a storage period of 5 days at 4 degrees C or at -20 degrees C. Recoveries of hCG in urine of a pregnant woman diluted with urine of a pre-menopausal non-pregnant woman (concentration range between 6 and 800 mU/L) were between 93% and 112% (y=0.997x-3.843, r 2 =0.999). Diluting a serum sample (hCG 42,000 U/L) with urine (negative for hCG) up to 8000-fold yielded a completely linear hCG response, indicating that the assay was not affected by the urine matrix. In a correlation study with 60 urine samples (of which 10 were of male origin), we did not find any discrepancies between results for the manual pregnancy test and the hCG test on the Roche Modular E170 (using a cutoff value of 50 U/L).

  20. Clinical performance of the new Roche COBAS TaqMan HCV Test and High Pure System for extraction, detection and quantitation of HCV RNA in plasma and serum

    NARCIS (Netherlands)

    Gelderblom, Huub C.; Menting, Sandra; Beld, Marcel G.


    We evaluated the Roche COBAS TaqMan HCV Test For Use With The High Pure System (TaqMan HPS; Roche Diagnostics), for the extraction, detection and quantitation of hepatitis C virus (HCV) RNA in serum or plasma of HCV-infected individuals. The TaqMan HPS is a real-time PCR assay with a reported linear

  1. Comparison of the Bactec Peds Plus pediatric blood culture vial with Roche pediatric Septi-Chek for blood cultures from pediatric patients.


    Welby, P L; Zusag, T M; Storch, G A


    Equal volumes of blood from 4,112 blood cultures were inoculated into one Bactec Peds Plus bottle and one Roche Septi-Chek bottle. There were no significant differences in the recoveries of bloodstream pathogens. Initial detection occurred earlier with Bactec Peds Plus, while growth on solid media occurred earlier with Roche Septi-Chek.

  2. Can the Roche hemolysis index be used for automated determination of cell-free hemoglobin? A comparison to photometric assays. (United States)

    Petrova, Darinka Todorova; Cocisiu, Gabriela Ariadna; Eberle, Christoph; Rhode, Karl-Heinz; Brandhorst, Gunnar; Walson, Philip D; Oellerich, Michael


    The aim of this study was to develop a novel method for automated quantification of cell-free hemoglobin (fHb) based on the HI (Roche Diagnostics). The novel fHb method based on the HI was correlated with fHb measured using the triple wavelength methods of both Harboe [fHb, g/L = (0.915 * HI + 2.634)/100] and Fairbanks et al. [fHb, g/L = (0.917 * HI + 2.131)/100]. fHb concentrations were estimated from the HI using the Roche Modular automated platform in self-made and commercially available quality controls, as well as samples from a proficiency testing scheme (INSTAND). The fHb using Roche automated HI results were then compared to results obtained using the traditional spectrophotometric assays for one hundred plasma samples with varying degrees of hemolysis, lipemia and/or bilirubinemia. The novel method using automated HI quantification on the Roche Modular clinical chemistry platform correlated well with results using the classical methods in the 100 patient samples (Harboe: r = 0.9284; Fairbanks et al.: r = 0.9689) and recovery was good for self-made controls. However, commercially available quality controls showed poor recovery due to an unidentified matrix problem. The novel method produced reliable determination of fHb in samples without interferences. However, poor recovery using commercially available fHb quality control samples currently greatly limits its usefulness. © 2013.

  3. Racialized Spaces in Teacher Discourse: A Critical Discourse Analysis of Place-Based Identities in Roche Bois, Mauritius (United States)

    Wiehe, Elsa M.


    This eleven-month ethnographic study puts critical discourse analysis in dialogue with postmodern conceptualizations of space and place to explore how eight educators talk about space and in the process, produce racialized spaces in Roche Bois, Mauritius. The macro-historical context of racialization of this urban marginalized community informs…

  4. Evaluation of a third party enzymatic ammonia method for use on the Roche Cobas 6000 (c501) automated platform. (United States)

    Seiden-Long, Isolde; Schnabl, Kareena; Skoropadyk, Wendy; Lennon, Nola; McKeage, Arlayne


    Adaptation of the Randox Enzymatic Manual UV Ammonia method to be used on the Roche Cobas 6000 (c501) automated analyzer platform. The Randox ammonia reagent was evaluated for precision, linearity, accuracy and interference from hemolysis, icterus and lipemia on the Roche c501 analyzer. Comparison studies were conducted for the Randox reagent between Roche c501, Siemens Vista, Ortho Vitros 250, and Beckman DxC methods. The Randox reagent demonstrates acceptable within-run (L1=65 μmol/L, CV 3.4% L2=168 μmol/L, CV 1.9%) and between-run precision (L1=29 μmol/L, CV 7.3% L2=102 μmol/L, CV 3.0%), Analytical Measurement Range (7-940 μmol/L), and accuracy. The method interference profile is superior for the Randox method (hemolysis index up to 600, icteric index up to 60, lipemic index up to 100) as compared to the Roche method (hemolysis index up to 200, icteric index up to 10, lipemic index up to 50). Comparison was very good between the Randox reagent and two other wet chemistry platforms. The Randox Enzymatic Manual UV Ammonia reagent is an available alternative to the Roche Cobas c501 reagent. The method is more robust to endogenous interferences and less prone to instrument error flags, thus allowing the majority of clinical specimens to be reported without additional sample handling at our institution. Copyright © 2014 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  5. Constraining Roche-Lobe Overflow Models Using the Hot-Subdwarf Wide Binary Population (United States)

    Vos, Joris; Vučković, Maja


    One of the important issues regarding the final evolution of stars is the impact of binarity. A rich zoo of peculiar, evolved objects are born from the interaction between the loosely bound envelope of a giant, and the gravitational pull of a companion. However, binary interactions are not understood from first principles, and the theoretical models are subject to many assumptions. It is currently agreed upon that hot subdwarf stars can only be formed through binary interaction, either through common envelope ejection or stable Roche-lobe overflow (RLOF) near the tip of the red giant branch (RGB). These systems are therefore an ideal testing ground for binary interaction models. With our long term study of wide hot subdwarf (sdB) binaries we aim to improve our current understanding of stable RLOF on the RGB by comparing the results of binary population synthesis studies with the observed population. In this article we describe the current model and possible improvements, and which observables can be used to test different parts of the interaction model.

  6. Pharmacological promiscuity: dependence on compound properties and target specificity in a set of recent Roche compounds. (United States)

    Peters, Jens-Uwe; Schnider, Patrick; Mattei, Patrizio; Kansy, Manfred


    The term "pharmacological promiscuity" describes a compound's pharmacological activity at multiple targets. Pharmacological promiscuity is undesired in typical drug discovery projects, which focus on the "one drug-one target" paradigm. Off-target activity can lead to adverse drug reactions, or can obscure pharmacodynamic effects in animal models. Therefore, advanced lead compounds, pharmacological tool compounds, and drug candidates are usually screened against panels of safety-relevant targets to detect unwanted pharmacological activities. To identify determinants of pharmacological promiscuity, we compared the panel screening outcomes of 213 recent Roche compounds with their molecular properties. Pronounced promiscuity was not observed below a threshold Clog P value of 2. For basic compounds, the propensity for weak off-target activity was found to increase with calculated basicities, whereas the potential for strong off-target activity depends more qualitatively on the presence of a positive charge at physiological pH. Compounds originating from projects with an aminergic receptor or transporter as a therapeutic target are particularly prone to promiscuity; the promiscuity of such compounds is mainly caused by their activity at other aminergic targets in the screening panel.

  7. Numerical Simulations of Collisional Cascades at the Roche Limits of White Dwarf Stars (United States)

    Kenyon, Scott J.; Bromley, Benjamin C.


    We consider the long-term collisional and dynamical evolution of solid material orbiting in a narrow annulus near the Roche limit of a white dwarf. With orbital velocities of 300 {km} {{{s}}}-1, systems of solids with initial eccentricity e≳ {10}-3 generate a collisional cascade where objects with radii r ≲ 100{--}300 {km} are ground to dust. This process converts 1-100 km asteroids into 1 μm particles in 102-106 yr. Throughout this evolution, the swarm maintains an initially large vertical scale height H. Adding solids at a rate \\dot{M} enables the system to find an equilibrium where the mass in solids is roughly constant. This equilibrium depends on \\dot{M} and {r}0, the radius of the largest solid added to the swarm. When {r}0 ≲ 10 km, this equilibrium is stable. For larger {r}0, the mass oscillates between high and low states; the fraction of time spent in high states ranges from 100% for large \\dot{M} to much less than 1% for small \\dot{M}. During high states, the stellar luminosity reprocessed by the solids is comparable to the excess infrared emission observed in many metallic line white dwarfs.

  8. Use of Roche coordinates in the problems of small oscillations of tidally-distorted stellar models. II

    International Nuclear Information System (INIS)

    Mohan, C.; Singh, V.P.


    Kopal's method of Roche coordinates used by the authors in an earlier paper (Mohan and Singh, 1978) to study the problems of small oscillations of tidally-distorted stars has been extended further to take into account the effect of second-order terms in tidal distortion. The results show that the effect of including terms of second order of smallness in tidal distortion in the metric coefficients of the Roche coordinates of tidally distroted stars is quite significant, especially in case of stars with extended envelopes and (or) larger values of the companion star producing tidal distortion. Some of the models which were earlier found stable against small perturbations now become dynamically unstable with the inclusion of the terms of second order of smallness in tidal effects. (Auth.)

  9. Pediatric reference intervals for 28 chemistries and immunoassays on the Roche cobas 6000 analyzer--a CALIPER pilot study. (United States)

    Kulasingam, Vathany; Jung, Benjamin P; Blasutig, Ivan M; Baradaran, Sanaz; Chan, Man Khun; Aytekin, Mujdat; Colantonio, David A; Adeli, Khosrow


    The aim of this study was to determine age- and sex-specific pediatric reference intervals for 28 analytes on the Roche cobas 6000 analyzer. The study was conducted at the Hospital for Sick Children in Toronto, Canada. Approximately 600 outpatient samples from a pediatric population deemed to be metabolically stable were subdivided into five age classes ranging from 0 to 20 years of age and further partitioned by gender. Reference intervals were established, after removal of samples significantly affected by hemolysis, icterus and lipemia and outlier exclusion, using the Robust statistical method to obtain the 2.5th and 97.5th percentiles. Age (birth to 20 years of age) and gender-appropriate pediatric reference intervals for 28 analytes are reported. These reference intervals provide the basis for clinical interpretation of laboratory results using the Roche cobas 6000 analyzer or related instrumentation/methods, provided adequate reference interval verification studies are performed. Copyright (c) 2010. Published by Elsevier Inc.

  10. El concepto de límite en el B-Mu de François Roche

    Directory of Open Access Journals (Sweden)

    Eugenio Pandolfini


    Full Text Available


    Algunos proyectos, como el Dusty relief/B‐mu (2002 de François Roche demuestran como edificios complejos, que toman distancia desde los modelos mecanicistas para referirse a nuevos paradigmas, se pueden interpretar y comprenderse  mejor gracias a un análisis perceptivo que acerca el proyecto de arquitectura a cuestiones como la relación psicológica del hombre con la arquitectura, el miedo al espacio, y las  patologías  vinculadas a la percepción y a las neurosis modernas.
    En este caso, aparte de las repercusiones que la fachada de  polvo podría tener en el ámbito de la ecología urbana, es interesante analizar algunos aspectos ligados a la dicotomía  entre forma externa y volúmenes internos para la que François Roche cita como referencia el raumplan de Adolf Loos, pero que presenta motivos para una reflexión vinculada a los aspectos  perceptivos.
    El artículo trata de analizar como François Roche proyecta sus edificios extremando la dicotomía entre interior/visual y  exterior/táctil, desarrollando así una nueva relación con el lugar. Roche diseña la fachada exterior del B‐mu autoimponiéndose  una limitación del sentido de la vista, a favor de una dimensión háptica del proyecto y lo hace envolviendo los ámbitos arquitectónicos más familiares de una interfaz abstracta y táctil.

    Palabras clave

    edificio, percepción, entorno, envolvente, límite


    Some projects such as Dusty relief/B‐mu (2002 by François  Roche demonstrate how complex buildings, which distance themselves from the mechanicist models in order to refer  themselves to new paradigms, can be better understood and interpreted thanks to a perceptive analysis.
    This analysis brings the architectural project closer to matters  such as man’s psychological relationship to Architecture, the fear of space

  11. Spurious hemolysis does not influence the reliability of digoxin testing on Siemens RXL MAX and Roche Cobas e601. (United States)

    Lippi, Giuseppe; Mercadanti, Mariella; Romero, Araelsis; Lipreri, Graziella; Salvagno, Gian Luca; Guidi, Gian Cesare


    Little information is available on the influence of spurious hemolysis on digoxin immunoassays. Seventeen consecutive, non-hemolyzed, sodium-heparin samples were divided in three aliquots. The first was immediately centrifuged and tested for hemolysis index (HI), as well as plasma digoxin on Siemens RXL MAX using the Siemens Dimension Flex and Roche Cobas e601 by electrochemiluminescent (ECLIA) technique. The second and third aliquots were subjected to mechanical hemolysis by aspirating the blood one and two times through a thin needle. The concentration of digoxin measured on Siemens RXL MAX was significantly decreased from aliquot #A, to aliquot #B (-4%), and aliquot #C (-6%), but in none of the hemolyzed specimens the 10% bias was exceeded. No significant variation was observed by measuring plasma digoxin on Roche Cobas.

  12. Polyphony and counterpoint: Mechanisms of seduction in the diaries of Helen Hessel and Henri Pierre Roché

    Directory of Open Access Journals (Sweden)

    Catherine du Toit


    Polifonie en kontrapunt: Meganismes van verleiding in die dagboeke van Helen Hessel en Henri Pierre Roché. Henri Pierre Roché (1879–1959, outeur van Jules et Jim, word beskryf as ‘n sosiale koppelaar, ‘n model-liefhebber van alles en nog wat, ‘n versamelaar van vroue en kuns en een van die mees produktiewe dagboekskrywers en aktiewe minnaars in die opgetekende geskiedenis. Roché het ‘n reeks sketse oor Don Juan gepubliseer en was geboei deur die figuur van die verleier. In sy twintigs beplan hy om sy lewe te wy aan die skepping van ‘n œuvre wat die morele, intellektuele, sosiale en seksuele verhoudings tussen mans en vrouens sou ondersoek. Ter bereiking van hierdie doel, rig hy sy lewe in as laboratorium waarin werklike ondervindinge dien as hoofbron van inligting. Sy dagboek strek oor sestig jaar en is ryk aan verhale van verleiding. Desnieteenstaande bly die mees intense en boeiende intrige van verleiding en verraad steeds sy verhouding met Helen Hessel. Aan die begin van hulle verhouding, stel Roché voor dat sy ook ‘n dagboek hou van hulle hartstogtelike liefde. Helen Hessel se dagboek, geskryf in Frans, Duits en Engels, reflekteer die drama van verleiding en funksioneer op verskillende vlakke: realisties, visionêr, ten volle geabsorbeer in haar eie gedagtes en emosies en tóg krities jeens haarself en ander. ‘n Vergelyking van die twee dagboeke skep ‘n fassinerende, digte tekstuur wat die binnewerkings blootlê van verleiding in aksie. Die kontrapunt geskep deur hierdie twee interafhanklike stemme word nóg meer kompleks namate ‘n mens bewus word van die intertekstuele verwysings wat bydra tot die ontluikende polifonie van geskrewe liefde en lewe.

  13. Numerical Simulations of Gaseous Disks Generated from Collisional Cascades at the Roche Limits of White Dwarf Stars (United States)

    Kenyon, Scott J.; Bromley, Benjamin C.


    We consider the long-term evolution of gaseous disks fed by the vaporization of small particles produced in a collisional cascade inside the Roche limit of a 0.6 {M}⊙ white dwarf. Adding solids with radius {r}0 at a constant rate {\\dot{M}}0 into a narrow annulus leads to two distinct types of evolution. When {\\dot{M}}0≳ {\\dot{M}}0,{crit}≈ 3× {10}4 {({r}0/1{km})}3.92 {{g}} {{{s}}}-1, the cascade generates a fairly steady accretion disk where the mass transfer rate of gas onto the white dwarf is roughly {\\dot{M}}0 and the mass in gas is {M}g≈ 2.3× {10}22 ({\\dot{M}}0/{10}10 {{g}} {{{s}}}-1) (1500 {{K}}/{T}0) ({10}-3/α ) g, where T 0 is the temperature of the gas near the Roche limit and α is the dimensionless viscosity parameter. If {\\dot{M}}0≲ {\\dot{M}}0,{crit}, the system alternates between high states with large mass transfer rates and low states with negligible accretion. Although either mode of evolution adds significant amounts of metals to the white dwarf photosphere, none of our calculations yield a vertically thin ensemble of solids inside the Roche limit. X-ray observations can place limits on the mass transfer rate and test this model for metallic line white dwarfs.

  14. Is confirmatory testing of Roche cobas 4800 CT/NG test Neisseria gonorrhoeae positive samples required? Comparison of the Roche cobas 4800 CT/NG test with an opa/pap duplex assay for the detection of N gonorrhoeae. (United States)

    Perry, Michael D; Jones, Rachel N; Corden, Sally A


    Recently marketed nucleic acid amplification tests (NAAT) for the detection of Neisseria gonorrhoeae (NG) have improved specificity over previous generation assays. A study to assess the necessity for confirmation of Roche cobas 4800 NG positive samples was undertaken by the Public Health Wales Microbiology Molecular Diagnostic Unit in Cardiff. Classical NG culture identification was compared to cobas 4800 (DR-9), opacity (opa) gene and porA pseudogene (pap) results. Confirmatory NAATs (opa/pap) were performed prospectively for 120 cobas 4800 NG positive urogenital and extragenital samples. Retrospective supplementary NAAT and sequence analysis of additional cobas 4800 NG positive extragenital samples was also carried out. Of the 188 classically identified clinical NG isolates, 184 were identified as NG in all 3 molecular targets. Two isolates were only detected by 2 molecular targets. A further 2 isolates were culture false-positives. Combining the results from prospective and retrospective testing, the sensitivity and negative predictive value for cobas 4800 NG detection for urogenital, rectal and oropharyngeal samples was 100%. Specificity for all sample types was greater than 99.7%. Positive predictive value was 96.0% and 96.4% for urogenital and rectal specimens, respectively, and 88.6% for oropharyngeal samples. Molecular tests could be used for culture confirmation where available. Roche cobas 4800 Chlamydia trachomatis/Neisseria gonorrhoeae (CT/NG)CT/NG gonorrhoea diagnosis is superior to culture with urogenital and rectal positives not requiring confirmation. Roche cobas 4800 oropharyngeal NG detection findings warrant further prospective study of routine confirmatory testing accounting for its cost and clinical usefulness. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to

  15. Assessment of the Roche Linear Array HPV Genotyping Test within the VALGENT framework. (United States)

    Xu, Lan; Oštrbenk, Anja; Poljak, Mario; Arbyn, Marc


    Cervical cancer screening programs are switching from cytology-based screening to high-risk (hr) HPV testing. Only clinically validated tests should be used in clinical practice. To assess the clinical performance of the Roche Linear Array HPV genotyping test (Linear Array) within the VALGENT-3 framework. The VALGENT framework is designed for comprehensive comparison and clinical validation of HPV tests that have limited to extended genotyping capacity. The Linear Array enables type-specific detection of 37 HPV types. For the purpose of this study, Linear Array results were designated as positive only if one of the 13 hrHPV types also included in the Hybrid Capture 2 (HC2) was detected. The VALGENT-3 framework comprised 1600 samples obtained from Slovenian women (1300 sequential cases from routine cervical cancer screening enriched with 300 cytological abnormal samples). Sensitivity for cervical intraepithelial neoplasia of grade 2 or worse (CIN2+) (n=127) and specificity for Linear Array and for HC2 and non-inferiority of Linear Array relative to HC2 was checked. In addition, the prevalence of separate hrHPV types in the screening population, as well as the concordance for presence of HPV16, HPV18 and other hrHPV types between Linear Array and the Abbott RealTime High Risk HPV test (RealTime) were assessed. The clinical sensitivity and specificity for CIN2+ of the Linear Array in the total study population was 97.6% (95% CI, 93.3-99.5%) and 91.7% (95% CI, 90.0-93.2%), respectively. The relative sensitivity and specificity of Linear Array vs HC2 was 1.02 [95% CI, 0.98-1.05, (pLinear Array in the screening population was 10.5% (95% CI, 8.9-12.3%) with HPV16 and HPV18 detected in 2.3% and 0.9% of the samples, respectively. Excellent agreement for presence or absence of HPV16, HPV18 and other hrHPV between Linear Array and RealTime was observed. Linear Array showed similar sensitivity with higher specificity to detect CIN2+ compared to HC2. Detection of 13 hrHPV types

  16. Near-complete genome sequencing of swine vesicular disease virus using the Roche GS FLX sequencing platform

    DEFF Research Database (Denmark)

    Nielsen, Sandra Cathrine Abel; Bruhn, Christian Anders Wathne; Samaniego Castruita, Jose Alfredo


    of the capacity of a Roche GS FLX sequencing platform. Sequences were initially verified through one of two criteria; either a match between a de novo assembly and a reference mapping, or a match between all of five different reference mappings performed against a fixed set of starting reference genomes...... with significant genetic distances within the same species of viruses. All reference mappings used an iterative method to avoid bias. Further verification was achieved through phylogenetic analysis against published SVDV genomes and additional Enterovirus B sequences. This approach allows high confidence...

  17. Meticulous plasma isolation is essential to avoid false low-level viraemia in Roche Cobas HIV-1 viral load assays. (United States)

    Mortier, Virginie; Vancoillie, Leen; Dauwe, Kenny; Staelens, Delfien; Demecheleer, Els; Schauvliege, Marlies; Dinakis, Sylvie; Van Maerken, Tom; Dessilly, Géraldine; Ruelle, Jean; Verhofstede, Chris


    Pre-analytical sample processing is often overlooked as a potential cause of inaccurate assay results. Here we demonstrate how plasma, extracted from standard EDTA-containing blood collection tubes, may contain traces of blood cells consequently resulting in a false low-level HIV-1 viral load when using Roche Cobas HIV-1 assays. The presence of human DNA in Roche Cobas 4800 RNA extracts and in RNA extracts from the Abbott HIV-1 RealTime assay was assessed by quantifying the human albumin gene by means of quantitative PCR. RNA was extracted from plasma samples before and after an additional centrifugation and tested for viral load and DNA contamination. The relation between total DNA content and viral load was defined. Elevated concentrations of genomic DNA were detected in 28 out of 100 Cobas 4800 extracts and were significantly more frequent in samples processed outside of the AIDS Reference Laboratory. An association between genomic DNA presence and spurious low-level viraemia results was demonstrated. Supplementary centrifugation of plasma before RNA extraction eliminated the contamination and the false viraemia. Plasma isolated from standard EDTA-containing blood collection tubes may contain traces of HIV DNA leading to false viral load results above the clinical cutoff. Supplementary centrifugation of plasma before viral load analysis may eliminate the occurrence of this spurious low-level viraemia.

  18. An evaluation of the New Roche Diagnostics Kit for the rapid identification of clinically important non-dextrose, non-fastidious gram-negative rods. (United States)

    Elegbe, I A


    The evaluation of the new Roche Diagnostics Commercial kit (Roche Diagnostics, 1975) for the identification of non-dextrose fermenting, non-fastidious gram-negative rods has been compared with conventional methods in the recognition and identification of these non-dextrose, non-fastidious gram-negative bacteria. This new kit has definite advantages over and above the conventional methods in a number of ways. It is cheaper to run, and above all it is less cumbersome, less time consuming and it is accurate. Apart from all these, the new method makes use of the odour test (Roche Diagnostics, 1975) and other additional conventional tests recommended by the Oxi/ferm manufacturer.

  19. Testing single-grain quartz OSL methods using sediment samples with independent age control from the Bordes-Fitte rockshelter (Roches d'Abilly site, Central France)

    DEFF Research Database (Denmark)

    Thomsen, Kristina Jørkov; Murray, Andrew Sean; Buylaert, Jan-Pieter


    We present quartz single-grain dose distributions for four well-bleached and unmixed sediment samples with independent age control (22–48 ka), from the archaeologically important Bordes-Fitte rockshelter at Roches d'Abilly, France. This site has previously been dated using 14C AMS dating and stan......We present quartz single-grain dose distributions for four well-bleached and unmixed sediment samples with independent age control (22–48 ka), from the archaeologically important Bordes-Fitte rockshelter at Roches d'Abilly, France. This site has previously been dated using 14C AMS dating...

  20. Characterization of the scope and magnitude of biotin interference in susceptible Roche Elecsys competitive and sandwich immunoassays. (United States)

    Trambas, Christina; Lu, Zhong; Yen, Tina; Sikaris, Ken


    Background Biotin interference is a significant problem to which at-risk laboratories must now be attuned. We sought to systematically characterize the nature of this interference in Roche immunoassays. Methods Known concentrations of biotin were titrated into serum samples and the effects on competitive and sandwich immunoassays were analysed. The maximum and minimum concentrations examined reflect those likely to be achieved in individuals on 5 to 10 mg supplements at the lower end, and 100 to 300 mg biotin at the high end. Results A high variability in biotin tolerance was observed. Some assays, such as troponin T, TSH and antithyroid antibodies, were extremely sensitive to the lower concentrations of biotin (15.6 and 31.3 ng/mL), whereas the majority of assays were relatively resistant. At concentrations ≥500 ng/mL, all assays showed significant interference from biotin but, again, the magnitude of the interference was variable. The more sensitive assays showed profound analytical bias at biotin concentrations that occur with high-dose therapy. Conclusion Our data demonstrate high variability in biotin tolerance across Roche immunoassays. The shape of the dose-response curves provides more detailed information than the single manufacturer-quoted figure for biotin tolerance. Accordingly, these data may be used by laboratories for more accurate risk assessment in predicting the effects of biotin. Our data may also be extrapolated to guide timing of blood tests in patients on high-dose biotin therapy: it demonstrates the number of half-lives required to withhold biotin in order to decrease its concentration to below a given assay tolerance.

  1. Next-generation sequencing of miRNAs with Roche 454 GS-FLX technology: steps for a successful application. (United States)

    Soares, Ana Raquel; Pereira, Patrícia M; Santos, Manuel A S


    MicroRNAs (miRNAs) are a class of small RNAs (sRNAs) of approximately 22 nucleotides in length that control eukaryotic gene expression at the translational level. They regulate a wide variety of biological processes, namely developmental timing, cell differentiation, cell proliferation, the immune response, and infection. Their identification is essential to understand eukaryotic biology. Their small size, low abundance, and high instability complicated early identification, however new generation genome sequencing approaches, such as the Roche 454 Pyrosequencer, allow for both miRNA identification and for generating miRNA profiles in a given sample. This technique avoids cloning steps in bacteria and is a fast and bias-minimized tool to discover novel miRNAs and other sRNAs on a genome-wide scale. Prior to sequencing, cDNA libraries are built for each sample using total RNA as starter material. Each cDNA library can be tagged with specific identifier sequences that allow sequencing different samples in the same chip run. Here, we describe the protocols for the construction of sRNA cDNA libraries for 454 sequencing, and we include tips for overcoming problems often encountered during cDNA library preparation.

  2. Contribution à l'étude de la "taille" des roches Contribution to the Study of Rock Cutting

    Directory of Open Access Journals (Sweden)

    Nguyenminh D.


    Full Text Available Une étude expérimentale détaillée du processus local de destruction des roches par un outil de forage pétrolier a été faite par une méthode de similitude utilisant des matériaux équivalents tendres. On examine l'influence de la forme de l'outil, de la rugosité du contact et de la ductilité e globale » du matériau. Les résultats obtenus sont ensuite comparés à ceux de l'analyse plastique par la théorie des charges limites. Cette étude met en évidence l'existence de deux modes de rupture, l'action différente du frottement superficiel selon le sens de l'écoulement relatif du matériau en contact, le phénomène de la ductilité apparente qui dépend de la forme de l'outil ; elle précise les paramètres mécaniques importants du forage. A detailed experimental investigation of the local process of rock cutting by a drill bit was carried out on soft equivalent materials simulating rock under borehole conditions. Different factors were analyzed : the shape of the bit tooth, the roughness of ifs contact area, the average ductility of the materiat. The results obtained are then compared with those from a plastic analysis based on the peak lood theory. This investigation shows that there are two different modes of rock destruction, that the Coulomb friction has a different effect depending on the relative direction of flow of the materiat in contact with the bit, and that an apparent ductility of the materiat is linked to the shape of the bit teeth. Finally, the basic mechanical variables for drilling are described

  3. Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

    Directory of Open Access Journals (Sweden)

    Hideyuki Tamaki

    Full Text Available BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the outcome of microbial community analyses. The aim of this study is to compare differences in output, ease, and cost among three different amplicon pyrosequencing methods for the Roche/454 Titanium platform METHODOLOGY/PRINCIPAL FINDINGS: The following three pyrosequencing methods for 16S rRNA genes were selected in this study: Method-1 (standard method is the recommended method for bi-directional sequencing using the LIB-A kit; Method-2 is a new option designed in this study for unidirectional sequencing with the LIB-A kit; and Method-3 uses the LIB-L kit for unidirectional sequencing. In our comparison among these three methods using 10 different environmental samples, Method-2 and Method-3 produced 1.5-1.6 times more useable reads than the standard method (Method-1, after quality-based trimming, and did not compromise the outcome of microbial community analyses. Specifically, Method-3 is the most cost-effective unidirectional amplicon sequencing method as it provided the most reads and required the least effort in consumables management. CONCLUSIONS: Our findings clearly demonstrated that alternative pyrosequencing methods for 16S rRNA genes could drastically affect sequencing output (e.g. number of reads before and after trimming but have little effect on the outcomes of microbial community analysis. This finding is important for both researchers and sequencing facilities utilizing 16S rRNA gene pyrosequencing for microbial ecological studies.

  4. Roche-lobe overflow systems powered by black holes in young star clusters: the importance of dynamical exchanges

    Energy Technology Data Exchange (ETDEWEB)

    Mapelli, Michela; Zampieri, Luca, E-mail: [INAF-Osservatorio Astronomico di Padova, Vicolo dell' Osservatorio 5, I-35122, Padova (Italy)


    We have run 600 N-body simulations of intermediate-mass (∼3500 M {sub ☉}) young star clusters (SCs; with three different metallicities (Z = 0.01, 0.1, and 1 Z {sub ☉}). The simulations include the dependence of stellar properties and stellar winds on metallicity. Massive stellar black holes (MSBHs) with mass >25 M {sub ☉} are allowed to form through direct collapse of very massive metal-poor stars (Z < 0.3 Z {sub ☉}). We focus on the demographics of black hole (BH) binaries that undergo mass transfer via Roche lobe overflow (RLO). We find that 44% of all binaries that undergo an RLO phase (RLO binaries) formed through dynamical exchange. RLO binaries that formed via exchange (RLO-EBs) are powered by more massive BHs than RLO primordial binaries (RLO-PBs). Furthermore, the RLO-EBs tend to start the RLO phase later than the RLO-PBs. In metal-poor SCs (0.01-0.1 Z {sub ☉}), >20% of all RLO binaries are powered by MSBHs. The vast majority of RLO binaries powered by MSBHs are RLO-EBs. We have produced optical color-magnitude diagrams of the simulated RLO binaries, accounting for the emission of both the donor star and the irradiated accretion disk. We find that RLO-PBs are generally associated with bluer counterparts than RLO-EBs. We compare the simulated counterparts with the observed counterparts of nine ultraluminous X-ray sources. We discuss the possibility that IC 342 X-1, Ho IX X-1, NGC 1313 X-2, and NGC 5204 X-1 are powered by an MSBH.

  5. Combination therapy containing ritonavir plus saquinavir has superior short-term antiretroviral efficacy: a randomized trial

    DEFF Research Database (Denmark)

    Kirk, O; Katzenstein, T L; Gerstoft, J


    trial. Two hundred and eighty-four patients started randomized treatment. The primary end-point was the proportion of patients with HIV RNA of 200 copies/ml or less (Roche Amplicor) and HIV RNA of 20 copies/ml or less (Roche ultradirect assay) at 6 months. Analysis was performed as intent...

  6. Multicenter evaluation of the new Abbott RealTime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA

    NARCIS (Netherlands)

    Schutten, M.; Peters, D.; Back, N. K. T.; Beld, M.; Beuselinck, K.; Foulongne, V.; Geretti, A.-M.; Pandiani, L.; Tiemann, C.; Niesters, H. G. M.


    The analytical performances of the new Abbott RealTime hepatitis C virus (HCV) and human immunodeficiency virus type 1 viral load assays were compared at nine laboratories with different competitor assays. These included the Abbott LcX, Bayer Versant bDNA, Roche COBAS Amplicor, and Roche COBAS

  7. Multicenter evaluation of the new Abbott RealTime assays for quantitative detection of human immunodeficiency virus type 1 and hepatitis C virus RNA

    NARCIS (Netherlands)

    Schutten, Martin; Peters, D; Back, N K T; Beld, M; Beuselinck, K; Foulongne, V; Geretti, A-M; Pandiani, L; Tiemann, C; Niesters, H G M

    The analytical performances of the new Abbott RealTime hepatitis C virus (HCV) and human immunodeficiency virus type 1 viral load assays were compared at nine laboratories with different competitor assays. These included the Abbott LcX, Bayer Versant bDNA, Roche COBAS Amplicor, and Roche COBAS

  8. Roche and IAEA announce joint initiative to train healthcare workers for Africa's fight against cancer. EDUCARE partnership to launch IAEA's VUCCnet training networks

    International Nuclear Information System (INIS)


    Full text: Roche and the International Atomic Energy Agency (IAEA) announced today the launch of the EDUCARE (EDUcation for Cancer in African REgions) project to provide concerted support to help combat the growing cancer epidemic in sub-Saharan Africa. The EDUCARE project is to be piloted in Ghana, Tanzania, Uganda and Zambia, and is linked with the IAEA's wider initiative to build regional training networks in cancer control and a Virtual University for Cancer Control (VUCCnet) in Africa. A core component for the successful fight against cancer in any country is the education and training of health care providers. The VUCCnet will allow for training to be provided in an integrated and sustainable way in Africa by taking advantage of low-cost online learning tools. The IAEA is working in collaboration with the World Health Organization (WHO) and other international partners to develop the VUCCnet across Africa. The EDCUARE project will facilitate a first-of-its-kind exchange of knowledge and skills, both at the healthcare provider and country-wide level. Training will be provided by an on-line training resource centre, known as the Virtual University for Cancer Control (VUCC), the first such platform for health workers across the continent. Maturin Tchoumi, General Manager Roche South Africa said: 'As a leader in oncology, Roche believes that its strengths, expertise and resources can be used to improve the quality of oncology training and education in the poorest countries in the world. There is a real lack of basic education in oncology in Africa. By contributing our skills and competencies on the ground, Roche can make a real and sustainable improvement.' This new public-private partnership reflects a shared concern over the increasing cancer burden in sub-Saharan Africa, a region of the world where cancer rates are growing rapidly. Cancer now accounts for 12.5% of all deaths worldwide, more than HIV/AIDS, TB and malaria combined. By 2020, there are expected to

  9. Alteration hydrothermale et deformation ductile des roches volcaniques acides associees au gisement sulfure de draa sfar (Jebilet Centrales, Maroc

    Directory of Open Access Journals (Sweden)

    Zinbi, Y.


    Full Text Available The volcanics and volcanoclastic rocks of Draa Sfar (Central Jebilet, Moroccan hercynian belt are affected by ductile stress and hydrothermal alteration accompanied by a weak degree of metamorphism (greenschist facies. Some N-S oriented shearing zones, affect locally these formations while being the site of an important hydrothermal activity. The consequences of these transformations from a non to slightly- deformed rhyodacite, show that through these ductile shearing zones: (1 the mineralogical assemblage of hydrothermal alteration is essentially formed by chlorite, sericite, quartz and magnetite; (2 the gradual increase of the alteration indexes is accompanied by the destruction of the phenocrists and the recrystallization of the matrix by phyllosilicates and quartz; (3 the progressive transfer of material is more intense in the more deformed zones where the values of Ti, Al and Zr remain constant. These shearing zones played a very important role in the circulation of fluids and the transformation of the rhyodacite of Draa Sfar.Les roches volcaniques et volcanoclastiques de Draa Sfar (Jebilet centrales, Maroc hercynien sont affectées par une déformation ductile accompagnée d’un métamorphisme de faible degré (faciès schistes verts et d’une altération hydrothermale. Des zones de cisaillement de direction N-S, ont affecté localement ces formations tout en étant vecteurs d’une importante activité hydrothermale. Le suivi de ces transformations à partir de la rhyodacite non ou peu déformée, montre qu’à travers ces zones de cisaillements ductiles : (1 l’assemblage minéralogique d’altération hydrothermale est formé essentiellement de chlorite, de séricite, de quartz et de magnétite ; (2 l’augmentation graduelle des indices d’altération s’exprime par la destruction des phénocristaux au profit d’une matrice recristallisée en phyllosilicates et quartz ; (3 le transfert progressif de la matière est plus intense

  10. Gyrotropy and Anisotropy of Rocks: Similarities and Differences Gyrotropie et anisotropie des roches : similitudes et différences

    Directory of Open Access Journals (Sweden)

    Chichinina T. I.


    Full Text Available The main features of wave propagation in gyrotropic media are compared with wave propagation in anisotropic media. The results of numerical modelling are presented for three typical seismic exploration models. The first two models are azimuthally anisotropic media (of orthorombic symmetry system, group 2m without and with gyration. The third model is a gyrotropic transversely isotropic medium with a vertical symmetry axis. The computations have been made for propagation of shear waves along the vertical symmetry axis. For sufficiently short wave paths (in our models less than 400 m the two-component (x, y seismograms are similar for all three models. For longer paths both signal shape and signal duration for the first and the third model differ noticeably. Some evidence (experimental data and a micromodel is given to show that the gyrotropy of rocks does exist or, at least, can exist. Les principales caractéristiques de la propagation des ondes dans les milieux gyrotropes sont comparées avec la propagation des ondes dans les milieux azimutalement anisotropes. Les résultats d'une modélisation numérique sont présentés pour trois modèles caractéristiques d'exploration sismique. Les deux premiers modèles sont des milieux anisotropes (de symétrie orthorhombique, groupe 2m avec et sans gyrotropie. Le troisième modèle est un milieu gyrotrope transverse isotrope avec un axe de symétrie vertical. Ces calculs ont été réalisés pour la propagation des ondes transversales le long de l'axe de symétrie vertical. Pour des trajets sismiques suffisamment courts (pour nos modèles, moins de 400 m, les sismogrammes à deux composantes (x, y sont similaires pour les trois modèles. Pour des trajets plus longs, la forme et la durée du signal diffèrent sensiblement pour les modèles 1 et 3. Ceci a pour but de montrer (à l'aide des données expérimentales et d'un micromodèle que la gyrotropie dans les roches existe, ou, tout au moins, peut

  11. From mass media to new media in contemporary Irish drama: Billy Roche's On Such As We and Paul Meade's Skin Deep

    Directory of Open Access Journals (Sweden)

    Patrick Lonergan


    Full Text Available This article explores the impact of new media and the mass media on the production, composition and reception of contemporary Irish drama. It considers the emergence of several tensions in that genre, notably that between mobility and stasis and the local and the global. This development is considered in relation to a discussion of two plays: Billy Roche's On Such As We, which was produced at the Peacock Theatre in 2001, and Paul Meade's Skin Deep, premiered by his company Gúna Nua at the Project Arts Centre in Dublin in 2003.

  12. Aprovechamiento del salto de Bourg-Lés-Valence. La Presa de la Roche de Glun-Drôme – Francia

    Directory of Open Access Journals (Sweden)

    Française d´Enterprise, Compagnie


    Full Text Available To take the fullest advantage of the hydroelectric and agricultural usefulness of the Rhône, the Compagnie Nationale du Rhône has divided the river into a number of sections, and these have been separately developed. The hydraulic gradient at La Roche de Glun, at the end of the central section, involves a hydraulic system consisting of a reservoir, a dam and dykes, a canal, a power station, an overflow canal and drainage works in the , surrounding zones. This project has been carried out by the Compagnie Française d'Enterprises.Para explotar al máximo las posibilidades hidroeléctricas y agrícolas del Ródano, la Compagnie Nationale du Rhône ha dividido dicho río en una serie de tramos para proceder a su distribución. El salto de La Roche de Glun, último del tramo central, forma un complejo hidráulico que consta de: un embalse cerrado por diques, una presa, un canal de alimentación, una central, una esclusa, un canal de descarga y unas obras anejas de drenaje para evitar inundaciones en las ciudades próximas. Las obras han sido realizadas por la Compagnie Française d'Entreprises.

  13. A comparative evaluation between real time Roche COBas TAQMAN 48 HCV and bDNA Bayer Versant HCV 3.0. (United States)

    Giraldi, Cristina; Noto, Alessandra; Tenuta, Robert; Greco, Francesca; Perugini, Daniela; Spadafora, Mario; Bianco, Anna Maria Lo; Savino, Olga; Natale, Alfonso


    The HCV virus is a common human pathogen made of a single stranded RNA genome with 9600nt. This work compared two different commercial methods used for HCV viral load, the bDNA Bayer Versant HCV 3.0 and the RealTime Roche COBAS TaqMan 48 HCV. We compared the reproducibility and linearity of the two methods. Seventy-five plasma samples with genotypes 1 to 4, which represent the population (45% genotype 1; 24% genotype 2; 13% genotype 3; 18% genotype 4) were directly processed with the Versanto method based upon signal amplification; the same samples were first extracted (COBAS Ampliprep - TNAI) and then amplified using RealTime PCR (COBAS TaqMan 48). The results obtained indicate the same performance for both methods if they have genotype 1, but in samples with genotypes 2, 3 and 4 the RealTime PCR Roche method gave an underestimation in respect to the Bayer bDNA assay.

  14. Detection of genetically diverse human immunodeficiency virus type 1 group M and O isolates by PCR.


    Respess, R A; Butcher, A; Wang, H; Chaowanachan, T; Young, N; Shaffer, N; Mastro, T D; Biryahwaho, B; Downing, R; Tanuri, A; Schechter, M; Pascu, R; Zekeng, L; Kaptué, L; Gürtler, L


    A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140...


    International Nuclear Information System (INIS)

    Rappaport, Saul; Sanchis-Ojeda, Roberto; Winn, Joshua N.; Rogers, Leslie A.; Levine, Alan


    The requirement that a planet must orbit outside of its Roche limit gives a lower limit on the planet's mean density. The minimum density depends almost entirely on the orbital period and is immune to systematic errors in the stellar properties. We consider the implications of this density constraint for the newly identified class of small planets with periods shorter than half a day. When the planet's radius is accurately known, this lower limit to the density can be used to restrict the possible combinations of iron and rock within the planet. Applied to KOI 1843.03, a 0.6 R ⊕ planet with the shortest known orbital period of 4.245 hr, the planet's mean density must be ∼> 7 g cm –3 . By modeling the planetary interior subject to this constraint, we find that the composition of the planet must be mostly iron, with at most a modest fraction of silicates (∼< 30% by mass)

  16. Comparative study of the serum measurement of PTH on Roche Cobas e411® versus the Abbott Architect ci8200®. (United States)

    Bensalah, Mohammed; Bouayadi, Ouardia; Rahmani, Nawal; Lyagoubi, Amina; Lamrabat, Somiya; Choukri, Mohammed


    Parathormone (PTH) is the main hormone of phosphocalcic homeostasis. It is synthesized and secreted by the parathyroid glands. PTH has become a routine test in the medical biology laboratory. However, its measurement presents analytical difficulties with the various marketed kits. The aim of this work is to present the results of a comparative study between the PTH measurment on Abbott architect ci8200 and on Roche's Cobas e411 automaton. It is a prospective study carried out for 252 hospitalized patients in the various departments of the University Hospital Center Mohammed VI of Oujda. The "intact" PTH tests were performed on two automata: Abbott Architect ci8200 and Roche Cobas e411. The first uses chemiluminescent microparticle immunoassay. The second uses electrochemiluminiscence sandwich enzyme immunoassay. The agreement of the results between the different techniques was evaluated using the Bland-Altman difference diagram and the Passing-Bablok and Deming regression line (MedCalc software version ® ). By analyzing the diagram of Bland-Altman, we note that the average bias between both methods is of the order of 193.9 pg/mL. As for the equation of the right of Passing-Bablok, it is: Y(Architect) = 3.11 X (Cobas) - 12.26. In conclusion, our study shows a great discrepancy between the results of the PTH assay on the Architect ci8200 versus the Cobas e411, hence the biologist's indisputable role in the control and evaluation of the kits marketed through the various validation tests.

  17. De Sébastien Roch à L’agneau chaste ou de la construction à la déconstruction de la matrice romanesque de la pédophilie


    Szczur Przemysław


    L’obiettivo del presente articolo è di paragonare l’immagine della pedofilia in due romanzi : Sébastien Roch di Octave Mirbeau e L’agneau chaste di Franck Varjac. Mentre il primo ha contribuito a costruire la matrice romanzesca della pedofilia, il secondo prova a decostruirla.

  18. De Sébastien Roch à L’agneau chaste ou de la construction à la déconstruction de la matrice romanesque de la pédophilie

    Directory of Open Access Journals (Sweden)

    Szczur Przemysław


    Full Text Available L’obiettivo del presente articolo è di paragonare l’immagine della pedofilia in due romanzi : Sébastien Roch di Octave Mirbeau e L’agneau chaste di Franck Varjac. Mentre il primo ha contribuito a costruire la matrice romanzesca della pedofilia, il secondo prova a decostruirla.

  19. The Roche Total Mycophenolic Acid® assay: An application protocol for the ABX Pentra 400 analyzer and comparison with LC–MS in children with idiopathic nephrotic syndrome

    Directory of Open Access Journals (Sweden)

    François Parant


    Full Text Available Background: For TDM of mycophenolate acid (MPA, the Roche Total Mycophenolic Acid® assay based on the inhibition of recombinant inosine monophosphate dehydrogenase (IMPDH has been shown to be a simple and reliable alternative to chromatographic methods. We have adapted this assay on the ABX Pentra 400 analyzer (HORIBA. Objective: To investigate the analytical performances of the Roche Total Mycophenolic Acid® assay on the ABX Pentra 400 and to compare it to an LC-MS method using samples from children with nephrotic syndrome treated with mycophenolate mofetil (MMF. Material and methods: Configuration of the open-channel on the ABX Pentra 400 was based on the Roche MPA assay package insert. Precision was determined as described in the CLSI protocol EP5-A2. Comparison with the LC-MS method was performed using 356 plasma samples from 42 children with nephrotic syndrome (8 h pharmacokinetic profiles. Results: The enzymatic assay demonstrated high precision. The %CV for Within Run Imprecision ranged from 5.5% at 1.2 mg/L to 1.5% at 14.1 mg/L and Total Imprecision ranged from 9.3% to 2.5%. The method comparison with plasma samples from children yielded overall a good correlation and a good agreement between both methods. The Passing Bablok regression analysis showed the following results: [Roche MPA assay]=1.058 [MPA LC-MS] −0.06; rho=0.996. Conclusion: The Roche Total Mycophenolic Acid® assay is adaptable to the ABX Pentra 400 analyzer, and demonstrates accurate and precise measurement of MPA in plasma obtained from children with nephrotic syndrome. Keywords: Enzyme assay, Inosine monophosphate dehydrogenase, Mycophenolic acid, Nephrotic syndrome

  20. Apport des expériences de mécaniques des roches à la géologie structurale des bassins sédimentaires Contribution of Rock-Mechanics Experiments to the Stuctural Geology of Sedimentary Basins

    Directory of Open Access Journals (Sweden)

    Poulet M.


    Full Text Available Dans le but de préciser le domaine d'application des expériences de Mécanique des Roches à la Géologie Structurale, l'auteur définit d'abord les ordres de grandeur des différents facteurs qui contrôlent, dans la nature, les relations contrainte déformation : contraintes mises en leu, vitesse de déformation, température, anisotropie des roches. Sont ensuite passés en revue les résultats des essais de laboratoire de type uniaxial (conditions de surface puis de type triaxial (conditions de fond en distinguant dans chaque cas la nature des contraintes intervenant : tension, compression, cisaillement. Le comportement des roches dans les essais en compression permet de les classer en trois catégories : les roches dites élastiques (roches sédimentaires compactes, métamorphiques et cristallines, les roches dites élastoplastiques possédant une certaine porosité (grés, calcaires, les roches dites plastiques (sel, gypse. Sur chaque type ainsi défini les expériences de laboratoire permettent d'étudier l'influence des paramètres suivants : la pression de confinement qui augmente avec la profondeur et permet une déformation permanente importante des roches élasto-plastiques, la pression des fluides qui les rend au contraire plus fragiles, la température dont l'influence est prédominante pour les roches plastiques, le temps qui seul permet des déformations continues importantes sous de faibles contraintes, l'anisotropie liée soit au dépôt (stratification, soit aux déformations antérieures. Bien que les caractéristiques mécaniques mesurées en laboratoire ne soient pas transposables directement dans la nature, les résultats des essais fournissent au géologue une clef pour l'interprétation qualitative de nombreuses observations à petite et grande échelle : relations socle-couverture sédimentaire, conditions de rejeu des accidents anciens, formation des plis, répartition et nature de la tracturation. In on attempt ta

  1. Performance evaluation of cobas HBV real-time PCR assay on Roche cobas 4800 System in comparison with COBAS AmpliPrep/COBAS TaqMan HBV Test. (United States)

    Kim, Hanah; Hur, Mina; Bae, Eunsin; Lee, Kyung-A; Lee, Woo-In


    Hepatitis B virus (HBV) nucleic acid amplification testing (NAAT) is important for the diagnosis and management of HBV infection. We evaluated the analytical performance of the cobas HBV NAAT (Roche Diagnostics GmbH, Mannheim, Germany) on the cobas 4800 System in comparison with COBAS AmpliPrep/COBAS TaqMan HBV Test (CAP/CTM HBV). Precision was evaluated using three levels of cobas HBV/HCV/HIV-1 Control Kit, and linearity was evaluated across the anticipated measuring range (10.0-1.0×109 IU/mL) at seven levels using clinical samples. Detection capability, including limit of blank (LOB), limit of detection (LOD) and limit of quantitation (LOQ), was verified using the 4th WHO International Standard for HBV DNA for NAT (NIBSC code: 10/266). Correlation between the two systems was compared using 205 clinical samples (102 sera and 103 EDTA plasma). Repeatability and total imprecision (coefficient of variation) ranged from 0.5% to 3.8% and from 0.5% to 3.5%, respectively. Linearity (coefficient of determination, R2) was 0.999. LOB, LOD and LOQ were all acceptable within the observed proportion rate (85%). Correlation was very high between the two systems in both serum and plasma samples (correlation coefficient [r]=0.995). The new cobas HBV real-time PCR assay on the cobas 4800 System showed reliable analytical performances.

  2. Evaluation of the Roche LightMix®Gastro Parasites multiplex PCR assay detecting Giardia duodenalis, Entamoeba histolytica, cryptosporidia, Dientamoeba fragilis, and Blastocystis hominis. (United States)

    Friesen, Johannes; Fuhrmann, Jörg; Kietzmann, Heike; Tannich, Egbert; Müller, Michael; Ignatius, Ralf


    Multiplex PCR assays offer highly sensitive and specific tools for the detection of enteric pathogens. This prospective study aimed at comparing the novel Roche LightMix ® Modular Assay Gastro Parasites (LMAGP) detecting Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, and Dientamoeba fragilis with routine laboratory procedures. Stool specimens (n=1062 from 1009 patients) were consecutively examined by LMAGP, R-Biopharm Ridascreen ® enzyme immunoassays (EIAs) detecting G. duodenalis or E. histolytica/dispar, and microscopy of wet mounts. Discrepant results were analyzed by in-house PCR. D. fragilis or B. hominis were detected by LMAGP in 131 (14.4%) and 179 (19.9%; 16 samples positive by microscopy; P PCR). G. duodenalis was detected by LMAGP, EIA, or microscopy in 20, 16, or 9 of 1039 stool samples, respectively; all four samples missed by EIA were confirmed by in-house PCR. In total, 938 stool samples were analyzed for E. histolytica/dispar. Nine of ten EIA-positive samples were negative by LMAGP but positive by in-house PCR for E. dispar. One E. histolytica infection (positive by both LMAGP and in-house PCR) was missed by EIA and microscopy. Parasites only detected by microscopy included Enterobius vermicularis eggs (n = 3) and apathogenic amoebae (n = 27). The data call for routine use of multiplex PCR assays for the detection of enteric protozoan parasites in laboratory diagnostics. Copyright © 2018. Published by Elsevier Ltd.

  3. Diffusion Properties of Carbonated Caprocks from the Paris Basin Propriétés diffusives de roches de couverture carbonatées du Bassin de Paris

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    Berne P.


    carbonated caprocks (regardless of the problem of alteration by acidified water, not addressed here. Lastly, a few simple rules for extending the results presented here to other experimental conditions are proposed. L’efficacite d’un stockage geologique de CO2 repose sur les proprietes de confinement des roches de couverture. Parmi ces proprietes, les caracteristiques diffusives jouent un role important qui est parfois sous-estime. En effet, la diffusion est un processus lent mais qui peut s’averer non negligeable sur le long terme. De plus, les caracteristiques recherchees pour une roche de couverture, principalement une forte pression d’entree et une faible permeabilite, ne sont pas incompatibles avec des coefficients de diffusion relativement eleves, puisque ces grandeurs sont liees a des caracteristiques geometriques differentes (schematiquement, les tailles de pore pour les unes et la tortuosite pour les autres. Dans le cadre du projet Geocarbone–INTEGRITE, finance par l’ANR de 2006 a 2008, on a donc mesure les coefficients de diffusion de l’eau et de l’ion bicarbonate dans des echantillons de roche de couvertures carbonatees provenant de trois niveaux (formations des Calcaires du Comblanchien et de la Dalle Nacree. Ces deux especes permettent en effet de couvrir, en premiere approximation, les situations rencontrees en conditions de stockage (espece neutre/espece chargee, interagissant ou non avec la matrice solide. Dans un premier temps, on presente le modele simple de diffusion utilise pour le depouillement des experiences, ce qui permet de passer en revue les differentes grandeurs utiles : coefficient de diffusion effectif, coefficient de diffusion de pore, coefficient de diffusion apparent, porosite accessible, facteur de retard. Le coefficient d’autodiffusion de l’eau est mesure par deux techniques differentes (Resonance Magnetique Nucleaire et tracage radioactif a l’eau tritiee 1H3HO dans deux echantillons. Les resultats obtenus sont

  4. The Bolivian Source Rocks. Sub Andean Zone. Madre de Dios. Chaco Les roches mères de Bolivie. Subandin. Madre de Dios. Chaco

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    Diaz Martinez E.


    connaissances sur les roches mères de Bolivie. Ce travail a été fait dans le cadre de la convention de coopération qui lie depuis 1987 YPFB et l'ORSTOM - Institut Français de Recherche Scientifique pour le Développement en Coopération. L'établissement de cette base de données a permis une synthèse du potentiel pétrolier du pays, la partie concernant le bassin de Madre de Dios, la zone subandine et le Chaco, c'est-à-dire toute la zone potentiellement pétrolière du pays, hors Altiplano, est ici présentée. La base contient actuellement les analyses d'un peu plus de 3000 échantillons. Les roches mères sont Paléozoïques et se sont déposées dans un contexte de bassin d'avant-pays; celui ci s'étendait sur la cordillère ainsi que sur l'avant-pays actuel du Silurien au Permien. Le bassin, restreint initialement à la zone sud-ouest, s'est ensuite étendu vers le nord. La bordure ouest n'est pas connue, elle devait se situer au niveau de l'Altiplano actuel. La bordure est correspond au bouclier Brésilien affleurant actuellement à l'est du pays et sur laquelle se biseaute le Paléozoique. La sédimentation est marine, il s'agit d'une plate-forme carbonatée s'approfondissant au Carbonifère. De nombreuses évidences de diamectites et de figures de resédimentation ont été notées à partir de la fin du Dévonien. Le Trias marque la fin de ces dépôts et se caractérise par une érosion, sans doute due à une phase de rifting avortée. La sédimentation Jurassique et Crétacée est presque toujours continentale et souvent éolienne, il n'y a pas de structuration marquée. Au nord, dans la zone de Madidi, les dépôts du Crétacé terminal (Maestrichian montrent des intercalations marines riches en matière organique (Fm Flora, alors que son équivalent au centre et au Sud (Fm Cajones est purement gréseux. Le soulèvement de la cordillère orientale démarre à la fin de l'Oligocène (27 Ma, Sempere et al. , 1990 et le front de compression se déplace ensuite

  5. Avancées en mécanique des roches expérimentales : étude expérimentale de la propagation des fissures sous l'action du gel dans les massifs calcaires


    BOST, Marion; POUYA, Ahmad; GUEDON, Sylvine; MARTINEAU, François


    Étude expérimentale de la propagation des fissures sous l'action du gel dans les massifs calcaires : Enjeux du gel dans les roches. Études de faisabilité. Dispositif et protocole expérimentaux. Analyse et modèle phénoménologique. Modèle empirique de la contrainte générée.

  6. 10Be dating of the Main Terrace level in the Amblève valley (Ardennes, Belgium): new age constraint on the archaeological and palaeontological filling of the Belle-Roche palaeokarst (United States)

    Rixhon, Gilles; Bourlès, Didier L.; Braucher, Régis; Siame, Lionel; Cordy, Jean-Marie; Demoulin, Alain


    It is still disputed whether very old archaeological and palaeontological remains found in the Belle-Roche palaeocave (eastern Belgium) pertain to the Early (˜1 Ma) or Middle (˜0.5 Ma) Pleistocene. Here, in situ-produced cosmogenic 10Be concentrations from a depth profile in nearby sediments of the Belle-Roche terrace (Amblève Main Terrace level) are used as an indirect solution of this chronological issue. The distribution of 10Be concentrations in the upper 3 m of this profile displays the theoretically expected exponential decrease with depth. Assuming a single exposure episode, we obtain a best fit age of 222.5±31 ka for the time of terrace abandonment. However, below 3 m, the 10Be concentrations show a marked progressive increase with depth. This distinctive cosmogenic signal is interpreted as the result of slow aggradation of the fluvial deposits over a lengthy interval. Modelling of the whole profile thus suggests that the onset of the terrace formation occurred at around 550 ka, with a sediment accumulation rate of ˜20 mm/ka. Based on two slightly different reconstructions of the geomorphic evolution of the area and a discussion of the temporal link between the cave and Main Terrace levels, we conclude that the fossil-bearing layers in the palaeokarst pertain most probably to MIS 14-13 (or possibly MIS 12-11) and the artifact-bearing layer to MIS 13 (or possibly MIS 11). This age estimate for the large mammal association identified in the Belle-Roche palaeokarst and the attribution to MIS 14-13 of a similar fauna found in the lowermost fossiliferous layers of the Caune de l'Arago (Tautavel) are in mutual support. Our results therefore confirm the status of the Belle-Roche site as a reference site for the Cromerian mammal association and the Early Palaeolithic industry in NW Europe.

  7. A comparison of Roche Kinetic Interaction of Microparticles in Solution (KIMS) assay for cannabinoids and GC-MS analysis for 11-nor-9-carboxy-delta9-tetrahydrocannabinol. (United States)

    Lyons, T P; Okano, C K; Kuhnle, J A; Bruins, M R; Darwin, W D; Moolchan, E T; Huestis, M A


    In this study, we investigated the effectiveness of the Roche Kinetic Interaction of Microparticles in Solution (KIMS) screening assay for cannabinoid metabolites. Urine specimens (N = 1689) were collected during elimination of cannabinoids from 25 subjects with a history of marijuana use. Specimens were analyzed concurrently for cannabinoid metabolites by a customized Department of Defense (DOD) cannabinoid KIMS kit (50-ng/mL cutoff) and for 11-nor-9-carboxy-delta9-tetrahydrocannabinol (THC-COOH) by GC-MS (15-ng/mL cutoff). As compared to GC-MS results, the sensitivity, specificity, and efficiency of the KIMS assay were 69.7%, 99.8%, and 88.6%, respectively. Many of the false-negative results had GC-MS concentrations between 15 and 26 ng/mL (N = 151). The cannabinoid screening results for the DOD samples tested by the laboratory during the same 8-month period were also evaluated. The linear regression analyses of GC-MS results in the 15-50 ng/mL range and KIMS data resulted in regression coefficients of 0.689 for the research specimens and 0.546 for DOD specimens. The results suggest that the KIMS cannabinoid screening assay is deficient in detecting positives around the cutoff (15-25 ng/mL THC-COOH). This limitation of the KIMS cannabinoid screening method compromises the identification of true positive specimens, therefore reducing the effectiveness of the assay. The success of the DOD program is dependent on sensitive and specific screening assays; the high prevalence of false-negative cannabinoid results compromises the program's primary objective of drug deterrence.

  8. Apports de l’archéobotanique à la compréhension de la Source des Roches, Chamalières (Puy-de-Dôme The archaeobotanic contribution to the understanding of la Source des Roches, Chamalières (Puy-de-Dôme

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    Béatrice Prat


    Full Text Available Au tout début de la période romaine, un taux d’arbres inhabituellement élevé a été mis en évidence par la palynologie sur le site de la Source des Roches à Chamalières (Puy-de-Dôme, gisement qui a livré plus de 3500 ex-voto en bois au début des années 70. Durant le second âge du Fer et l’époque romaine, un tel niveau de boisement n’est observé nulle part ailleurs, ni en plaine de Limagne, ni sur le plateau des Dômes. L’hypothèse d’un sanctuaire de plein air, soutenue par Anne-Marie Romeuf qui a dirigé la fouille, se trouve ainsi confortée par l’analyse pollinique, qui permet d’envisager la possibilité de la présence d’un « bois sacré » composé principalement de chênes. La combinaison des données archéobotaniques issues de la palynologie et de la carpologie contribue à renforcer la compréhension du site d’un point de vue paysager et nous renseigne également sur les types d’offrandes végétales associées au dépôt des ex-voto.Unusually high levels of tree pollen were revealed by palynology on the site of the Source des Roches in Chamalières (Puy-de-Dôme for the very beginning of the Roman period. The site had yielded more than 3500 wooden offerings in the early 70s. Such a high density of woodland can be found nowhere else for the Second Iron Age and the Roman period, neither in the plain of Limagne, nor on the plateau of Les Dômes. The hypothesis of an open air sanctuary, first advanced by Anne-Marie Romeuf, director of excavation, is therefore reinforced by the pollen analysis which leads us to consider the possibility that a “sacred grove” made up principally of oaks was present. The correlation of the archaeobotanic data from palynology and paleœthnobotany contributes to the strengthening of the site’s knowledge from the landscape’s point of view. We also have a better understanding of the vegetal offerings associated with the wooden statues.

  9. Performance verification and comparison of TianLong automatic hypersensitive hepatitis B virus DNA quantification system with Roche CAP/CTM system. (United States)

    Li, Ming; Chen, Lin; Liu, Li-Ming; Li, Yong-Li; Li, Bo-An; Li, Bo; Mao, Yuan-Li; Xia, Li-Fang; Wang, Tong; Liu, Ya-Nan; Li, Zheng; Guo, Tong-Sheng


    To investigate and compare the analytical and clinical performance of TianLong automatic hypersensitive hepatitis B virus (HBV) DNA quantification system and Roche CAP/CTM system. Two hundred blood samples for HBV DNA testing, HBV-DNA negative samples and high-titer HBV-DNA mixture samples were collected and prepared. National standard materials for serum HBV and a worldwide HBV DNA panel were employed for performance verification. The analytical performance, such as limit of detection, limit of quantification, accuracy, precision, reproducibility, linearity, genotype coverage and cross-contamination, was determined using the TianLong automatic hypersensitive HBV DNA quantification system (TL system). Correlation and Bland-Altman plot analyses were carried out to compare the clinical performance of the TL system assay and the CAP/CTM system. The detection limit of the TL system was 10 IU/mL, and its limit of quantification was 30 IU/mL. The differences between the expected and tested concentrations of the national standards were less than ± 0.4 Log 10 IU/mL, which showed high accuracy of the system. Results of the precision, reproducibility and linearity tests showed that the multiple test coefficient of variation (CV) of the same sample was less than 5% for 10 2 -10 6 IU/mL; and for 30-10 8 IU/mL, the linear correlation coefficient r 2 = 0.99. The TL system detected HBV DNA (A-H) genotypes and there was no cross-contamination during the "checkerboard" test. When compared with the CAP/CTM assay, the two assays showed 100% consistency in both negative and positive sample results (15 negative samples and 185 positive samples). No statistical differences between the two assays in the HBV DNA quantification values were observed ( P > 0.05). Correlation analysis indicated a significant correlation between the two assays, r 2 = 0.9774. The Bland-Altman plot analysis showed that 98.9% of the positive data were within the 95% acceptable range, and the maximum difference

  10. Gamma-emissions of some meteorites and terrestrial rocks. Evaluation of lunar soil radioactivity; Emissions gamma de quelques meteorites et roches terrestres. Evaluation de la radioactivite du sol lunaire

    Energy Technology Data Exchange (ETDEWEB)

    Nordemann, D. [Commissariat a l' Energie Atomique, Centre d' Etudes Nucleaires de Saclay, 91 - Gif-sur-Yvette (France)


    The gamma-emissions of some terrestrial rocks and of the following meteorites: Bogou, Eagle-Station, Granes, and Dosso were studied by quantitative low background gamma spectrometry. These measurements and their interpretation lead to the evaluation of the possible gamma-emissions of several models of lunar soils. (author) [French] Les emissions gamma des meteorites Bogou, Eagle-Station, Granes et Dosso et de quelques roches terrestres ont ete etudiees par spectrometrie gamma quantitative a faible mouvement propre. Ces mesures et leur interpretation permettent d'evaluer les principales contributions des emissions gamma du sol lunaire pour des modeles de compositions possibles variees. (auteur)

  11. Les roches basiques des boutonnieres d'Agadir melloul et d'Iguerda - taifast : tkmoins de l’histoire preorogenique de la chaine panafricaine de l’Anti-Atlas (Maroc

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    El Aouli, E. H.


    Full Text Available Dans les boutonnières précambriennes d'Agadir Melloul et d'Iguerda-Taïfast (Anti-Atlas central, les roches basiques du Néoprotérozoïque (PII sont représentées par des dykes et des corps basiques intrusifs dans le socle paléoprotérozoïque (PI et les quartzites du PII. L’ensemble PI, PII et roches basiques est affecté par la schistosité régionale panafricaine. Les éléments en traces, réputés peu mobiles au cours des processus d'altération et de métamorphisme (Nb, Y, Zr, Ti, V et les terres rares ont permis de subdiviser ces roches basiques en deux groupes: un groupe tholeiitique et un groupe transitionnel. Le cadre géodynamique de mise en place de ces roches pourrait être lié à un contexte distensif marquant le rifting pré-panafricain en relation avec l’ouverture océanique reconnue dans la boutonnière de Bou Azzer-El Grara et dans le massif de Siroua.En los complejos precámbricos de Agadir Melloul y de Iguarda-Taifast (Anti-Atlas centro, las rocas básicas del Neoproterozoico (PII están representadas por diques y cuerpos básicos intmsivos en el zócalo Paleoproterozoico (PI y las cuarcitas del PII. El conjunto PI, PII y las rocas básicas está afectado por la esquistosidad regional panafricana. Los elementos traza incompatibles, poco móviles en el transcurso de procesos de alteración y de metamorfismo (Nb, Y, Zr, Ti, V, así como las tierras raras, permiten subdividir estas rocas básicas en dos grupos: un grupo toleítico y otro transicional. El marco geodinámico de la intrusion de estas rocas podria estar ligado a un contexto extensional asociado al rifting pre-panafricano en relación con la apertura oceánica reconocida en el complejo de Bou Azzer-El Graara y en el macizo de Siroua.

  12. The clinical effectiveness and cost-effectiveness of rituximab for the first-line treatment of chronic lymphocytic leukaemia: an evidence review of the submission from Roche. (United States)

    Main, C; Pitt, M; Moxham, T; Stein, K


    This paper presents a summary of the evidence review group (ERG) report into the clinical effectiveness and cost-effectiveness of rituximab for the first-line treatment of chronic lymphocytic leukaemia (CLL) based upon a review of the manufacturer's submission to the National Institute for Health and Clinical Excellence (NICE) as part of the single technology appraisal (STA) process. The manufacturer's searches for clinical effectiveness and cost-effectiveness data were appropriate and included all relevant studies. The submission's evidence came from a single, unpublished, well-conducted randomised controlled trial (RCT) comparing rituximab in combination with fludarabine and cyclophosphamide (R-FC) with fludarabine and cyclophosphamide (FC) alone for the first-line treatment of CLL. There was a statistically significant increase in progression-free survival (PFS) with R-FC compared with FC alone {median 39.8 months vs 32.2 months; hazard ratio [HR] 0.56 [95% confidence interval (CI) 0.43 to 0.72]}. However, the initial significant treatment benefit for R-FC compared with FC for overall survival was not maintained at a slightly longer follow-up time [median 25.4 months; adjusted HR 0.72 (95% CI 0.48 to 1.09)]. Response rates, numbers of patients with event-free survival and duration of response all favoured treatment with R-FC. Additional evidence from a mixed-treatment comparison model indicated R-FC to be significantly superior to chlorambucil alone for both PFS and overall and complete response rates. The incidence of grade 3 or 4 adverse events was higher in the R-FC arm (77%) than in the FC arm (62%). Dose modifications were also more frequent in this arm, but this did not lead to differences in treatment discontinuation. Roche used a three-state Markov model (PFS, progressed and death) to model the cost-effectiveness of R-FC compared with FC and chlorambucil alone. The model used a cycle length of 1 month and a lifetime time horizon. The approach taken to

  13. Analyse géochimique de la matiére organique extraite des roches sédimentaires. IV. Extraction des roches en faible quantités Geochemical Analysis of Organic Matter Extracted from Sedimentary Rocks Iv. Exraction from Small Amounts of Rock

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    Monin J. C.


    Full Text Available L'extraction en Soxhlet est inappllcable lorsque les échantillons de roche sont de trop petite taille. A l'occasion de la mise au point du protocole d'extraction correspondant, on examine l'influence d'un certain nombre de conditions opératoires sur le rendement d'extraction : température, durée nature et quantité du solvant, présence de lumière, présence d'air, procédé d'extraction. Pour les hydrocarbures, tant saturés qu'aromatiques, le facteur essentiel est l'agitation du milieu d'extraction ; la nature du solvant n'est pas critique, à condition de ne pas choisir un très mauvais solvant des hydrocarbures : l'extractibilité est en effet plus fonction du pouvoir désorbant vis-à-vis de la roche que du pouvoir solvant proprement dit. Pour les résines et asphalténes, l'interprétation des résultats est délicate, car la frontière n'est pas nette entre produits simplement dissous, produits de solvolyse et, produits de néoformation par interaction solvant-matière organique-matière minérale. II n'existe donc pas de protocole d'extraction recommandable dans l'absolu. Tout dépend des exigences analytiques et aussi pratiques du laboratoire; à l'Institut Français du Pétrole (IFP le protocole retenu est l'extraction en bécher avec agitation magnétique pendant 20 min dans le chroroforme à 50 °C (approximativement; on donne aussi le protocole d'évaporation du solvant et de récupération de l'extrait, qui doit être étudié soigneusement étant donné les faibles quantités mises en jeu. A Soxhlet extractor cannot be used with rock samples that are too small in size. With the development of on extraction procédure for such cases, this article examines the influence of various operating conditions on extraction yield, i. e. temperature, duration, nature and amount of solvent, presence of light, présence of air and extraction process. For both saturated and aromatic hydrocarbons, the essential factor is the stirring of the

  14. Indices chimiques pour l'évaluation de l'état diagénétique des huiles et des roches sapropéliques Chemical Evidence for Evaluating the Diagenetic State of Sapropelic Oils and Rocks

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    Du Rouchet J.


    Full Text Available Les analyses chromatographiques des vapeurs produites par le chauffage à 220 °C d'huiles brutes et de roches mettent en évidence une augmentation avec la diagenèse des teneurs en n-alcanes relativement aux teneurs en cyclones et en iso-alcanes. Une étude statistique permet de préciser la regularite de l'evolution des rapports des teneurs en n-C 6 et en méthylcyclopentane etdes teneurs en n-C 7 et en I-trans-2-diméthylcyclopentane et met en évidence pour les roches une corrélation significative entre ces rapports et les réflectances de la vitrinite. Ces rapports peuvent donc être considérés comme des indicateurs diagénétiques; ils paraissent être moins dépendants des caractéristiques particulières de la matière organique originelle que les couples pristane-n-C 17 et phytane-n-C 18 habituellement utilisés pour repérer l'état d'évolution des huiles et des extraits de roche. Une calibration en termes de réflectance de la vitrinite est proposée. Chromatographic analyses of the vapors produced by crude cils and rocks heated to 220°C reveal, with diagenesis, an increase in the n-alkane contents with respect to the cyclone and isoalkane contents. A statistical survey shows the regularity of the variation in the ratios of the n-C 6 and methylcyclopentane contents and the n-C7 and I-trans-2-dimethylcyclopentane contents and reveals a meaningful corrélation for rocks between these ratios and the vitrinite reflectances. Therefore, these ratios may be considered as diagenetic indicators. They appear to depend less on the particular properties of the original organic motter thon on the pristane/n-C 17 and phytane/n-C 18 pairs normally used to determine the state of evolution of ails and of rock extracts. A calibration in terms of vitrinite reflectance is proposed.

  15. Mechanical Properties of Rocks: Pore Pressure and Scale Effects Propriétés mécaniques des roches : pression de pore et effets d'échelle

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    Gueguen Y.


    Full Text Available Pore pressure plays a major role when considering rocks mechanical properties. In that field, the concept of effective pressure is a key one to deal with fluids mechanical effects. However, its frequent use has been the source of frequent confusing statements. Because of the various meanings which have been attached to that concept, an attempt is made in this paper to clarify it and examine the validity of its various uses relative to rock mechanical behaviour or rock properties. At a macroscopic scale, thermodynamics provides a powerful tool to investigate this. Reversible or irreversible thermodynamics provide general relationships of great interest. But because real rocks are non homogeneous systems, a microscopic approach is also required in order to analyze the mechanical properties from a description of the small scale processes. The microscopic approach is complementary of the macroscopic thermodynamic one as it leads to the calculation of the effective properties of the medium. In this last approach, effective medium theory is a powerful tool. The effective properties as derived from the microscale can be nicely combined to thermodynamic relations to interpret pore fluid pressure effects and scale effects. The example of elastic properties of porous rocks is more specifically emphasized to illustrate this because of both its intrinsic interest and importance as far as applications are concerned. La pression de pore joue un rôle de première importance dans la considération des propriétés mécaniques des roches. Dans ce domaine, le concept de contrainte effective est essentiel pour aborder les effets mécaniques. Toutefois, son utilisation fréquente a conduit à de nombreuses affirmations trompeuses. Compte tenu des significations diverses accordées à ce concept, nous tentons ici de le clarifier et examinons le domaine d'application de ses divers emplois dans le cadre du comportement mécanique ou des propriétés des roches. À l

  16. Une méthode précise pour la mise en évidence et l'étude de l'anisotropie dans les roches An Accurate Method for Detecting and Analyzing Anisotropy in Rocks

    Directory of Open Access Journals (Sweden)

    Talebi S.


    Full Text Available L'objectif essentiel de cette recherche était de mettre au point une méthode expérimentale pour étudier l'anisotropie de la vitesse des ondes longitudinales (P dans les roches. Cette anisotropie aurait deux causes principales : d'une part, l'existence d'un réseau de pores, de fissures ou de microfissures, distribué de façon anisotrope dans la roche et d'autre part, la structure elle-même, dont l'anisotropie résulte de la nature des minéraux et de leur disposition. Dans un premier temps, nous avons commencé les essais sur des échantillons de carotte, forés dans trois directions perpendiculaires X, Y et Z d'un bloc de deux types de grès : grès des Vosges et grès de Fontainebleau. La vitesse du son a été mesurée sur 3 à 4 sections de carotte avec des intervalles de 30° (6 directions. Elles se distribuent nettement selon une ellipse, appelée l'ellipse d'anisotropie. Ces ellipses ont été tracées par mini-ordinateur et ont permis de mettre en évidence l'état d'anisotropie et l'homogénéité des carottes. La méthode est tellement précise qu'une erreur de repérage des axes sur un échantillon (Y de grès des Vosges a été détectée et certifiée à l'aide de direction des micas. Nous avons effectué le même essai sur les mêmes échantillons saturés sous vide. Une tendance générale à l'augmentation des vitesses, la diminution du degré d'anisotropie (le rapport de Vmax/Vmin et le changement de position de l'axe d'anisotropie a été observée. La comparaison des résultats secs et saturés permet d'avoir une idée de la nature des vides et de leur rôle dans l'anisotropie globale. Dans l'étape suivante de l'étude, nous avons mis au point une méthode de mesure sur les cubes à faces tronquées (66 faces au maximum de différents types de roches (2 grès, 2 marbres, 1 andésite, 1 balsate, et 1 calcaire. Les valeurs de vitesses des ondes (P ont été mesurées et reportées sur un diagramme de Schmidt. Ensuite

  17. Détermination de la mouillabilité d'un échantillon de roche-réservoir Determining the Wettability of a Resevoir-Rock Sample

    Directory of Open Access Journals (Sweden)

    Cuiec L.


    Full Text Available L'objet de cet article est la description d'une méthode de laboratoire permettant l'évaluation de la mouillabilité d'une roche-réservoir vis-à-vis d'un couple eau-huile. Dans une première partie, on donne une définition du paramètre mouillabilité et on précise les conditions requises pour le mesurer valablement, une analyse critique rapide des différentes méthodes de mesure est effectuée. Elle permet de justifier le choix d'une méthode qui est décrite dans la seconde partie. De nombreux exemples d'application sont donnés à titre d'illustration. The aim of this article is to describe a laboratory method for evaluating the wettability of a reservoir rock with regard ta a water-oil system.The first part gives a définition of the wettability parameter and specifiesthe conditions required for volidly measuring it. A brief critical analysis is made of different measuring methods. This onalysis justifies the choice of a method which is described in the second part. A great many application examples are given to illustrate this.

  18. Application of paper chromatography to the determination of some elements in minerals and rocks; Application de la chromatographie sur papier a la determination de certains elements dans les mineraux et les roches

    Energy Technology Data Exchange (ETDEWEB)

    Agrinier, H. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires


    This publication describes our work in applying paper chromatography to the fields of mineralogy and geology. We have studied the separation and determination of the following elements: Nb, Ta, Ti, Co, Cu, Mn, Sn, Ge, As, Sb, Bi, U, Th, Be, Au, Pb, Zn, V and Mo. The development of separation methods for these various elements has led us to solve many interference problems. These methods make it possible to separate and measure mineral components. They also enable us to search for trace elements in ores, rocks soils and carbonaceous materials. Finally let us point out that this technique may equally be used for analysis in very radioactive environment. (author) [French] Dans cette etude, nous etendons le champ d'application de la chromatographie sur papier aux domaines de la mineralogie et de la geologie. Nous avons etudie la separation et la determination des elements suivants: Nb, Ta, Ti, Co, Cu, Mn, Sn, Ge, As, Sb, Bi, U, Th, Be, Au, Pb, Zn, V et Mo. La mise au point des methodes de separation concernant ces differents elements nous a amenes a resoudre de nombreux problemes d'interference. Ces methodes permettent la separation et le dosage des constituants des mineraux, ainsi que la recherche d'elements en traces dans les minerais, les roches, les sols et les materiaux carbonnes. Signalons pour terminer que cette technique peut egalement trouver son utilisation dans le domaine de l'analyse en milieu tres radio-actif. (auteur)

  19. Improving clinical laboratory efficiency: a time-motion evaluation of the Abbott m2000 RealTime and Roche COBAS AmpliPrep/COBAS TaqMan PCR systems for the simultaneous quantitation of HIV-1 RNA and HCV RNA. (United States)

    Amendola, Alessandra; Coen, Sabrina; Belladonna, Stefano; Pulvirenti, F Renato; Clemens, John M; Capobianchi, M Rosaria


    Diagnostic laboratories need automation that facilitates efficient processing and workflow management to meet today's challenges for expanding services and reducing cost, yet maintaining the highest levels of quality. Processing efficiency of two commercially available automated systems for quantifying HIV-1 and HCV RNA, Abbott m2000 system and Roche COBAS Ampliprep/COBAS TaqMan 96 (docked) systems (CAP/CTM), was evaluated in a mid/high throughput workflow laboratory using a representative daily workload of 24 HCV and 72 HIV samples. Three test scenarios were evaluated: A) one run with four batches on the CAP/CTM system, B) two runs on the Abbott m2000 and C) one run using the Abbott m2000 maxCycle feature (maxCycle) for co-processing these assays. Cycle times for processing, throughput and hands-on time were evaluated. Overall processing cycle time was 10.3, 9.1 and 7.6 h for Scenarios A), B) and C), respectively. Total hands-on time for each scenario was, in order, 100.0 (A), 90.3 (B) and 61.4 min (C). The interface of an automated analyzer to the laboratory workflow, notably system set up for samples and reagents and clean up functions, are as important as the automation capability of the analyzer for the overall impact to processing efficiency and operator hands-on time.

  20. [Comparison between the centrifugation on MPA C10 (Roche Diagnostics) and the centrifugation according recommendations of GEHT (Groupe d'étude de l'hémostase et de la thrombose) for the daily hemostasis assays]. (United States)

    Flamant, Fabrice; Borg, Jeanne-Yvonne; Lenormand, Bernard; Le Cam-Duchez, Véronique


    Actually, many laboratories tend to acquire pre analytic automates to prepare specimens for analysis. For haemostasis, these pre analytical modules are not always in agreement with the recommendations from the Groupe d'étude de l'hémostase et de la thrombose (GEHT). For example in the MPA C10 module (Roche Diagnostics) the speed of centrifugation was not rather fast compared with the GEHT recommandations. Then, to be able to use this automate for routine coagulation assays, we compared results of Quick time, activated partial prothombin time, fibrinogen, factor II, factor V, factor VII, factor X and antithrombin levels and unfractioned heparin anti-Xa activity measurement after MPA (1,885 g - 999 sec) or GEHT (2,500 g - 900 sec) protocol of centrifugation. First, we verified platelet counts: in 82% of specimens, the platelet counts were under 10.10(9)/L after centrifugation on MPA module. Moreover, a good correlation was observed in all comparisons. Then we concluded the MPA C10 module was usable for routine coagulation tests.

  1. Comparative evaluation of Amplicor HIV-1 DNA test, version 1.5, by ...

    African Journals Online (AJOL)

    Human immunodeficiency virus (HIV) DNA polymerase chain reaction (PCR) test using venous blood sample has been used for many years in low resource settings for early infant diagnosis of HIV infection in children less than 18 months. The aim of this study was to evaluate and compare the performance characteristics of ...

  2. Hepatitis C virus detection in the semen of infected patients

    Directory of Open Access Journals (Sweden)

    Norma de Paula Cavalheiro

    Full Text Available Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa were separated with 45% and 90% Percoll gradients. The HCV-RNA in blood and semen fractions was extracted using the same protocol (AMPLICOR Roche and was detected using the qualitative Roche Amplicor test and by agarose gel electrophoresis, with ethidium bromide staining. The mean age of the patients was 40.7 years. Risk factors for the acquisition of HCV included injectable and inhaled drug use in six (42.8%, blood transfusion in four (28.6%, and no risk factors in four (28.6% patients. Genotype 1 was detected in 62% of the patients, followed by genotype 3 in 23% and genotype 2 in 15%. All blood samples were positive, regardless of the technique used for detection. All semen samples identified by Roche Amplicor and analyzed by agarose gel electrophoresis were negative. Among the 52 semen samples (total and fractions identified by the Roche Amplicor method, 45 (87% were inhibited. A negative result was recorded for one (1.9% total semen sample, one (1.9% leukocyte and four (7.7% seminal plasma fractions. Only one (1.9% sample of the spermatozoon fraction was positive. The results obtained suggested false-negative reactions for the semen samples.

  3. [Detection of Staphylococcus aureus resistant to methicillin (MRSA) by molecular biology (Cepheid GeneXpert IL, GeneOhm BD, Roche LightCycler, Hyplex Evigene I2A) versus screening by culture: Economic and practical strategy for the laboratory]. (United States)

    Laudat, P; Demondion, E; Jouannet, C; Charron, J; Chillou, C; Salaun, V; Mankikian, B


    Patients admitted in cardiac surgery and cardiac ICU at the Clinic Saint-Gatien (Tours) are screened for MRSA at the entrance by nasal swab and culture on blood agar and selective chromogenic medium made by addition of cefoxitin: BBL CHROMagar MRSA-II BD (result obtained at Day +1). We wanted to assess the molecular biology techniques available to obtain a result at day 0 for the majority of patients and to define an economic and practical strategy for the laboratory. We studied four molecular biology techniques: Cepheid GeneXpert (Cepheid) GeneOhm (BD), LightCycler (Roche) and Hyplex (I2A). Upon reception, nasal swabs were treated by culture, considered as reference, and one of the techniques of molecular biology, according to the manufacturer's notice. We conducted four studies between April 2008 and February 2009 to obtain a significant sample for each of them. By screening we mean a method that allows us to exclude MRSA carriage for patients waiting for surgery, and not to change patient management: for example, lack of isolation measures specific to entrance, no modification of antibiotic prophylaxis during surgery and no isolation measures in the immediate postoperative period. The criteria we considered for this evaluation were: (1) technician time: time to perform one or a series of sample(s) n=10 or more (about 2h for all techniques except GeneXpert 75min), level of skilled competences (no specific training for GeneXpert); (2) results: turnaround time (all molecular biology techniques), ease of reading and results interpretations (no specialized training required for GeneXpert), failure or not (12% of failure of internal controls for GeneOhm); (3) economic: cost for one or a series of sample(s) (n=10 or more), if we considered X as the reference culture cost (10 X Hyplex and LightCycler, 20 X and 40 X for GeneXpert GeneOhm); (4) NPV: 100% for GeneXpert and LightCycler. At same sensitivity, no technique, including culture, can solve alone our problem, which

  4. High-risk Human Papillomavirus Determination in Formalin-fixed, Paraffin-embedded Cervical Tissue Using the Roche Cobas 4800 System: A Comparative Study With Liquid-based Cytology. (United States)

    Tardío, Juan C; Cambero, Olivia; Sánchez-Estévez, Carolina; Sánchez-García, Ana B; Angulo, Fernando; Moreno, Amalia


    Roche cobas 4800 human papillomavirus (HPV) test is an automated real-time polymerase chain reaction-based system that allows the simultaneous detection of 14 human papillomavirus high-risk (HR-HPV) genotypes. This test is Food and Drug Administration approved since 2011 for HPV determination in liquid-based cytologic samples, but a clinically validated technique for formalin-fixed, paraffin-embedded (FFPE) tissue specimens is presently not commercially available. In our laboratory, we have developed an HPV detection procedure in FFPE tissue by cobas 4800 HPV test. In order to validate our method, we retrospectively studied 165 FFPE cervical biopsy and conization specimens with varied diagnoses from our files. In 50 of them, we contrasted the results with those obtained from simultaneous liquid-based cytologies from the same patients. Finally, seeking the possible complementary clinical usefulness of the procedure, we compared the HPV genotypes detected in cervical intraepithelial neoplasia grade 1 (CIN1)-diagnosed biopsies from 20 patients with a subsequent high-grade CIN (CIN2+) diagnosis with those from another group of 20 patients without a posterior CIN2+ diagnosis. Eighty-seven percent of the assays provided informative results. HR-HPV was detected in 28 of 32 (88%) invasive cervical squamous carcinomas. Coincidental HR-HPV genotypes were obtained in 32 of 50 (64%) cases with simultaneous cervical biopsy and liquid-based cytologic samples. A significant higher risk of progression to CIN2+ was found when HPV16 (P=0.022) or any HR-HPV genotype (P=0.037) was detected in CIN1 biopsies. The reported procedure provides an automated, technically time-saving, easy to integrate into laboratory routine, and reliable method of HR-HPV determination in FFPE specimens.

  5. Automated Extraction of Formalin-Fixed, Paraffin-Embedded Tissue for High-Risk Human Papillomavirus Testing of Head and Neck Squamous Cell Carcinomas Using the Roche Cobas 4800 System. (United States)

    Kerr, Darcy A; Sweeney, Brenda; Arpin, Ronald N; Ring, Melissa; Pitman, Martha B; Wilbur, David C; Faquin, William C


    -Testing for high-risk human papillomavirus (HR-HPV) in head and neck squamous cell carcinomas (HNSCCs) is important for both prognostication and clinical management. Several testing platforms are available for HR-HPV; however, effective alternative automated approaches are needed. -To assess the performance of the automated Roche cobas 4800 HPV real-time polymerase chain reaction-based system on formalin-fixed, paraffin-embedded HNSCC specimens and compare results with standard methods of in situ hybridization (ISH) and p16 immunohistochemistry. -Formalin-fixed, paraffin-embedded samples of HNSCC were collected from archival specimens in the Department of Pathology, Massachusetts General Hospital (Boston), and prepared using the automated system by deparaffinization and dehydration followed by tissue lysis. Samples were integrated into routine cervical cytology testing runs by cobas. Corresponding formalin-fixed, paraffin-embedded samples were evaluated for HR-HPV by ISH and p16 by immunohistochemistry. Discrepant cases were adjudicated by polymerase chain reaction. -Sixty-two HNSCC samples were analyzed using the automated cobas system, ISH, and immunohistochemistry. Fifty-two percent (n = 32 of 62) of formalin-fixed, paraffin-embedded tumors were positive for HR-HPV by cobas. Eighty-eight percent (n = 28 of 32) of cases were the HPV 16 subtype and 12% (n = 4 of 32) were other HR-HPV subtypes. Corresponding testing with ISH was concordant in 92% (n = 57 of 62) of cases. Compared with the adjudication polymerase chain reaction standard, there were 3 false-positive cases by cobas. -Concordance in HNSCC HR-HPV status between cobas and ISH was more than 90%. The cobas demonstrated a sensitivity of 100% and a specificity of 91% for detection of HR-HPV. Advantages favoring cobas include its automation, cost efficiency, objective results, and ease of performance.

  6. CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: direct validation using reference samples from the CALIPER cohort. (United States)

    Estey, Mathew P; Cohen, Ashley H; Colantonio, David A; Chan, Man Khun; Marvasti, Tina Binesh; Randell, Edward; Delvin, Edgard; Cousineau, Jocelyne; Grey, Vijaylaxmi; Greenway, Donald; Meng, Qing H; Jung, Benjamin; Bhuiyan, Jalaluddin; Seccombe, David; Adeli, Khosrow


    The CALIPER program recently established a comprehensive database of age- and sex-stratified pediatric reference intervals for 40 biochemical markers. However, this database was only directly applicable for Abbott ARCHITECT assays. We therefore sought to expand the scope of this database to biochemical assays from other major manufacturers, allowing for a much wider application of the CALIPER database. Based on CLSI C28-A3 and EP9-A2 guidelines, CALIPER reference intervals were transferred (using specific statistical criteria) to assays performed on four other commonly used clinical chemistry platforms including Beckman Coulter DxC800, Ortho Vitros 5600, Roche Cobas 6000, and Siemens Vista 1500. The resulting reference intervals were subjected to a thorough validation using 100 reference specimens (healthy community children and adolescents) from the CALIPER bio-bank, and all testing centers participated in an external quality assessment (EQA) evaluation. In general, the transferred pediatric reference intervals were similar to those established in our previous study. However, assay-specific differences in reference limits were observed for many analytes, and in some instances were considerable. The results of the EQA evaluation generally mimicked the similarities and differences in reference limits among the five manufacturers' assays. In addition, the majority of transferred reference intervals were validated through the analysis of CALIPER reference samples. This study greatly extends the utility of the CALIPER reference interval database which is now directly applicable for assays performed on five major analytical platforms in clinical use, and should permit the worldwide application of CALIPER pediatric reference intervals. Copyright © 2013 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  7. Sex-specific 99th percentiles derived from the AACC Universal Sample Bank for the Roche Gen 5 cTnT assay: Comorbidities and statistical methods influence derivation of reference limits. (United States)

    Gunsolus, Ian L; Jaffe, Allan S; Sexter, Anne; Schulz, Karen; Ler, Ranka; Lindgren, Brittany; Saenger, Amy K; Love, Sara A; Apple, Fred S


    Our purpose was to determine a) overall and sex-specific 99th percentile upper reference limits (URL) and b) influences of statistical methods and comorbidities on the URLs. Heparin plasma from 838 normal subjects (423 men, 415 women) were obtained from the AACC (Universal Sample Bank). The cobas e602 measured cTnT (Roche Gen 5 assay); limit of detection (LoD), 3ng/L. Hemoglobin A1c (URL 6.5%), NT-proBNP (URL 125ng/L) and eGFR (60mL/min/1.73m 2 ) were measured, along with identification of statin use, to better define normality. 99th percentile URLs were determined by the non-parametric (NP), Harrell-Davis Estimator (HDE) and Robust (R) methods. 355 men and 339 women remained after exclusions. Overallstatistical method used influenced URLs as follows: pre/post exclusion overall, NP 16/16ng/L, HDE 17/17ng/L, R not available; men NP 18/16ng/L, HDE 21/19ng/L, R 16/11ng/L; women NP 13/10ng/L, HDE 14/14ng/L, R not available. We demonstrated that a) the Gen 5 cTnT assay does not meet the IFCC guideline for high-sensitivity assays, b) surrogate biomarkers significantly lowers the URLs and c) statistical methods used impact URLs. Our data suggest lower sex-specific cTnT 99th percentiles than reported in the FDA approved package insert. We emphasize the importance of detailing the criteria used to include and exclude subjects for defining a healthy population and the statistical method used to calculate 99th percentiles and identify outliers. Copyright © 2017 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

  8. Diagraphies et roches-mères. Estimation des teneurs en carbone organique par la méthode CARBOLOG Well Logs and Source Rocks Assessing Organic Carbon Content by the Carbolog Technique

    Directory of Open Access Journals (Sweden)

    Carpentier B.


    Full Text Available La prise en compte des teneurs en matière organique des roches-mères joue un rôle important dans l'évaluation du potentiel pétrolier des bassins sédimentaires. CARBOLOG est une nouvelle méthode d'estimation du contenu organique des roches-mères développée à l'Institut Français du Pétrole (lFP. Cette méthode tire partie des propriétés physiques de la matière organique qui se caractérise par une faible vitesse de propagation du son ainsi que par une résistivité élevée. Ces propriétés physiques peuvent être combinées de manière à permettre une estimation in situ du contenu en matière organique du sédiment. Dans un puits donné, la teneur en carbone organique est estimée par un traitement prenant conjointement en compte les valeurs fournies par deux types de diagraphies couramment disponibles dans les forages pétroliers : le Soniqueet la Résistivité . Pour mettre en oeuvre CARBOLOG à l'échelle d'un bassin sédimentaire il est nécessaire de procéder à une calibration basée sur le traitement de puits pour lesquels des données analytiques de teneur en carbone organique existent. Afin d'être validé, CARBOLOG a été testé avec succès sur des exemples appartenant à des bassins différents et présentant des faciès variés. Les résultats obtenus indiquent clairement la capacité de la méthode à estimer les teneurs en carbone organique et à rendre compte en continu de leurs évolutions verticales, pour des roches-mères marines, immatures ou faiblement matures. D'autres tests sont actuellement en cours sur des environnements de dépôt différents et des formations plus matures de manière à définir les possibilités et les limites de CARBOLOG dans l'ensemble des contextes géologiques généralement rencontrés. For assessing the petroleum potential of sedimentary basins, it is very important to take the organic matter contained in source rocks into consideration. CARBOLOG is a new method developed by

  9. The Painful Shoulder | Roche | Continuing Medical Education

    African Journals Online (AJOL)

    The painful shoulder is commonly managed by the non-specialist. Continuing Medical Education Vol. 26 (8) 2008: pp. 398-402. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms and Conditions of Use · Contact AJOL · News.

  10. Peer Group Learning in Roche Pharma Development (United States)

    Boulden, George P.; De Laat, Richard


    Pharma Development has used action learning to help participants in their 360[degrees] feedback programme develop their leadership competencies. The article describes how the programme was designed, supported and run across four sites over a period of 2 years. The programme was systematically evaluated and found to be successful in meeting its…

  11. Observations sur les microfaciès des roches sédimentaires prélevées sur la marge armoricaine Observations on the Microfacies of Sedimentary Rock Samples from the Armorican Margin

    Directory of Open Access Journals (Sweden)

    Pastouret L.


    Full Text Available L'étude des dragages de roches et de sédiments effectués sur la marge armoricaine entre 47 et 48, de latitude Nord permet d'en préciser l'évolution paléogéographique et tectonique et de l'intégrer à celle du golfe de Gascogne. L'analyse de mi crofaciès des échantillons rocheux dont l'âge varie du Jurassique supérieur au Néogène met en évidence - d'une part, l'appartenance de la bordure nord de la marge armoricaine au domaine mésogéen au moins jusqu'au Crétacé moyen; - d'autre part, la permanence d'un régime de plate-forme carbonatée qui paraît fonctionner du Tithonique au Tertiaire inférieur, sur laquelle ont pu s'installer des édifices récifaux notamment au Crétacé moyen. Pendant toute cette période la sédimentation a compensé la subsidence; - enfin, l'accentuation de la subsidence à partir de l'Éocène (accompagnée d'une diminution de la production de calcium $ qui est vraisemblablement en relation avec les phénomènes tectoniques majeurs qui ont affecté à ce moment la bordure sud de la plaque européenne. A study of rock samples and sedimenis obtained by dredging from the Armorican Margin (47 ta 48° N brings new data on the paleogeographic and tectonic evolution of this ares in relation ta the formation of the Bay of Biscay. Microfacies ranging from the Upper Jurassic ta the Neogene show - the Armorican Margin until al least the Middle Cretaceous belongs to the Mesogean realm ; - carbonate platform regime prevails from the Tithonian to the Lower Tertiory with reef building occurring in the Middle Cretaceous. During this whole period in the area under consideration, sedimentation accounts for or even exceeds subsidence ; - the subsidence rate increases (and/or carbonate production decreases toward the end of the Eocene, and this may be related ta first order tectonic events offecting the southern part of the European plate.

  12. [Diagnosis of chlamydia trachomatis infections in women: urinary PCR compared to cervical culture and PCR on cervical swabs in high risk females.]. (United States)

    Olafsson, J H; Davidsson, S; Karlsson, S M; Palsdottir, R; Steingrimsson, O


    Diagnosis of Chlamydia trachomatis infections in women has traditionally depended on cell culture or enzyme linked immunoassay. Recently Polymerase Chain Reaction (PCR) has been shown to be more sensitive than these methods when performed on endocervical swabs. A total of 203 high risk females were enrolled in a comparative study of three methods for diagnosing C. trachomatis infections: McCoy cell culture and Amplicor(R) PCR on endocervical swabs and urine. Thirty four had positive cultures, 38 positive PCR from cervix and 37 had positive PCR on urine specimens. When discrepancy occurred, the leftover Amplicor(R) specimen was retested by Roche with Amplicor(R) and a primer for the Major Outer Membrane Protein (MOMP) gene. None was false positive in cell culture or in urinary PCR but two were false positive in cervical PCR. In all three tests, 32 were positive. The sensitivity of culture was 87%, 92% in cervical PCR and 95% in urinary PCR. The specificity was 100% in both culture and urinary PCR but 98% in cervical PCR. The results show that Amplicor(R) PCR performed on female urine is more sensitive and as specific as cell culture.

  13. Observations on the Primary Migration of Oil in the la Luna Source Rocks of the Maracaibo Basin, Venezuela Observations de la migration primaire de l'huile dans les roches mères de la Formation de La Luna dans le bassin de Maracaïbo, Venezuela

    Directory of Open Access Journals (Sweden)

    Talukdar S.


    Full Text Available In the present work, an integrated approach has been made to understand the primary migration of oil in the organic-rich, oil prone, predominantly carbonate source rocks of the La Luna Formation of the Maracaibo Basin. Geochemical data on source rock extracts and the simulation of oil generation by hydrous-pyrolysis experiments indicate that the primary migration and expulsion of oil began at an early stage of thermal maturity and that the bulk oil expulsion at peak generation could have been as high as 75%. Fluorescence and transmitted light petrography of the source rocks at different maturity levels show the dominant mode of oil expulsion through microfractures. It is interpreted from the geochemical and geological data that the primary migration of oil in the La Luna source rocks took place as a separate oil phase through microfractures caused by the excess fluid pressure due to hydrocarbon generation. The oils expelled from the La Luna source are enriched in saturated hydrocarbons and depleted in resins + asphaltenes compared to the La Luna rock extracts. However the distributions of n-alkanes, in the oils and the rock extracts are very similar, which suggest that fractionation of the n-alkanes did not occur during the primary migration. In the Maracaibo Basin, updip lateral primary migration of oil within the La Luna source system seem to have occurred for a considerable distance. Local internal migration has been observed at a relatively early stage of maturity. Cette étude consiste en une approche intégrée cherchant à comprendre la migration primaire de l'huile dans les roches mères de la Formation de La Luna dans le bassin de Maracaïbo, riches en matière organique, génératrices d'huile et à dominante carbonatée. Les données géochimiques sur des extraits de roches mères et la simulation de genèse d'huile par des expériences de pyrolyse en présence d'eau indiquent que la migration primaire et l'expulsion de l'huile ont

  14. Generation of Anomalous Internal Pressures in Source Rocks and Its Role in Driving Petroleum Migration La genèse de pressions internes anormales dans les roches mères et son rôle de poussée dans la migration du pétrole

    Directory of Open Access Journals (Sweden)

    Barker C.


    . La présence de gaz conduit à des comportements très différents et la pression de pores dans les roches mères inférieure à la normale à moins qu'il n'y ait une genèse suffisante de la matière organique en hydrocarbures pour la compenser. En l'absence d'une genèse continue (c'est-à-dire pour le méthane biogénique, le gaz se dissout régulièrement et les pressions chutent considérablement en dessous de la pression hydrostatique. A l'inverse, sur les élévations, les systèmes gazéifères ont tendance à la surpression et la pression peut atteindre la charge de fracturation de la roche. Le craquage thermique des bitumes qui restent dans une roche mère au cours de l'enfouissement va de pair avec une augmentation de volume et, dans un pore isolé, le système des pressions atteint des valeurs élevées. Il suffit qu'un pour cent des bitumes de type huile soit transformé en gaz pour que la pression atteigne la charge pour laquelle la fracturation de la roche est une conséquence inévitable.

  15. Méthode rapide de caractérisation des roches mètres, de leur potentiel pétrolier et de leur degré d'évolution Rapid Method for Source Rocks Characrerysation and for Determination of Petroleum Potential and Degree of Evolution

    Directory of Open Access Journals (Sweden)

    Espitalié J.


    Full Text Available Les études fondamentales réalisées sur les kérogènes à l'aide de diverses méthodes physico-chimiques d'analyse ont permis de mettre au point une méthode et un appareillage adaptés à l'exploration pétrolière. On expose dans cet article la mise au point de cette méthode et on montre, à l'aide des paramètres qu'elle permet d'obtenir, ses applications dans le domaine de l'exploration pétrolière - reconnaissance des différents types de roche mère et de leur potentiel pétrolier; - caractérisation de leur degré d'évolution (zone à huile - zone à gaz. On montre aussi que cette méthode convient particulièrement bien à l'estimation du rendement en huile des roches bitumineuses et à l'étude de la qualité et du rang des charbons. Fundamental research on kerogens by different physico-chemical analysis methods have led to the development of a method and equipment suited for petroleum exploration. This article describes the development of this method and, by means of the parameters it determines, shows how it con be applied in the field of petroleum exploration - exploring different types of source rock and their petroleum potential ; - characterizing their degree of evolution (ail zone/gas zone. This method is also shown ta be particularly suited for estimating the oil yield of cil shales and investigating the quality and classification of cool.

  16. Liposome encapsulation of the internal control for whole process quality assurance of nucleic acid amplification-based assays. (United States)

    Berg, E S; Skaug, K


    A system intended for whole process quality assurance of nucleic acid amplification assays was developed based on the use of liposomes as cell-mimicking vehicles for the internal control, allowing introduction of the internal control directly into the crude biological specimens. By the proof of principle testing, the Roche Cobas Amplicor CT assay was chosen as model system and the Roche CT/NG Internal Control was thus loaded into the liposomes. The liposome/DNA particles were spiked into a Chlamydia trachomatis-positive urine specimen. A quantitative "in-house" duplex real-time C. trachomatis PCR assay showed that liposomes having Blue Dextran 2000 polysaccharide co-entrapped were the most suited particles as they were efficiently deposited by the centrifugation carried out according to the Roche urine specimen preparation procedure. Furthermore, it was demonstrated that the liposome/DNA particles might be used for whole process quality assurance of Amplicor assay without major modifications of the assay protocol. An additional feature of the use of these liposomes was that the pellet became blue coloured and that might facilitate a thorough removal of the urine supernatant without increasing the risk of disturbance of the pellet. Principally, the liposome/internal control system is versatile and seems to be applicable for whole process quality control of amplification-based assays for detection of various pathogens.

  17. Étude de la mouillabilité des roches réservoir à l'échelle du pore par cryomicroscopie électronique à balayage Wettability of Reservoir Rock At the Pore Scale: Contribution of Cryo-Scanning Electron Microscopy

    Directory of Open Access Journals (Sweden)

    Fassi-Fihri O.


    Full Text Available Le but de cette étude est de caractériser, à l'échelle du pore, la mouillabilité des roches réservoir, en relation avec leur géométrie et/ou leur minéralogie. Cette caractérisation se fait, après congélation des échantillons, par l'observation de la distribution des fluides au sein du milieu poreux (saumure et huile brute, en microscopie électronique à balayage. Les expériences ont d'abord été effectuées sur des roches modèles parfaitement mouillables à l'eau, verre fritté et grès naturels. Certains de ces minéraux ont été rendus hydrophobes par greffage de silane. L'étude de ces systèmes a mis en évidence une corrélation entre la mouillabilité et la distribution des fluides. Puis, une roche réservoir (grès argileux de la formation de Brent, de mer du Nord connue comme étant de mouillabilité intermédiaire a été étudiée. Un travail précédent (étude de déplacements eau/huile par tomographie X avait abouti à la conclusion que si ces roches présentaient des hétérogénéités de mouillabilité, l'échelle de ces hétérogénéités devait être inférieure au millimètre. Les études de cryomicroscopie ont montré le caractère hydrophobe de la kaolinite, tandis que les illites, le quartz et les feldspaths sont préférentiellement mouillables à l'eau. L'imbibition spontanée d'huile pourrait ainsi être attribuée à l'existence au sein de la roche d'un réseau de kaolinite, tandis que l'imbibition spontanée de saumure serait due à l'existence d'un second réseau plus ou moins imbriqué avec le premier et constitué des autres minéraux. Un autre cas de roche réservoir a été étudié, à savoir un carbonate du Moyen-Orient. Les mésopores intergranulaires y ont été observés comme étant mouillables à l'huile tandis que les micropores restaient mouillables à l'eau. Dans ce cas, la mouillabilité intermédiaire de ces échantillons s'explique par la géométrie plutôt que par la min

  18. Étude numérique du couplage thermohydromécanique dans les roches. Influence des termes de couplage non linéaires pour un matériau isotrope linéaire Numerical Analysis of a Thermohydromechanical Coupling in Rocks. Influence of Nonlinear Coupling Terms on a Linear Isotropic Material

    Directory of Open Access Journals (Sweden)

    Henry J. P.


    Full Text Available Nous présentons dans ce travail une étude numérique basée sur la méthode des éléments finis, du comportement thermoporoélastique de certaines roches. Les trois effets de couplage : déformabilité de la roche, pression interstitielle et température sont pris en compte simultanément dans la résolution numérique. Une application simple sur un puits pétrolier en conditions axisymétriques est finalement présentée afin de dégager en particulier l'influence du terme de couplage convectif non linéaire, obtenu dans l'équation de diffusivité thermique, sur l'évolution de la température et de la pression interstitielle autour du forage. This article describes a finite-element method for solving the problem of nonlinear coupling between interstitial pressure and temperature during stress on a poroelastic rock. Such coupling phenomena occur during massive injection of cold water into a petroleum borehole for example. The implementation of such a numerical solution, used here with the assumption of small deformations, first requires a review of the behavior law of the material (Eq. 2. 2 and of the equations for hydraulic diffusivity (Eq. 2. 3 and thermal diffusivity (Eq. 2. 4. This last equation (2. 4 is the one containing the nonlinear coupling terms in Grad P Grad P and Grad T. Grad P. During simulation of flow at a high flow rate, these products can no longer be neglected as shown by the results in Fig. 2. The variational formulation of the problem is then determined in relation to the three equations for equilibrium, thermal diffusivity and hydraulic diffusivity. After geometric and temporal discretizations, this formulation leads to a finite-element calculating scheme resulting in the simultaneous solving of all three equations. This solution, based on the inversion of the system of equations (2. 15, requires the updating of the rigidity matrix at each time step to take nonlinear coupling into consideration. Calculations with an

  19. Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City, Vietnam. (United States)

    Tham, Nguyen thi; Hang, Vu thi Ty; Khanh, Trong Huu; Viet, Do Chau; Hien, Tran Tinh; Farrar, Jeremy; Chau, Nguyen van Vinh; van Doorn, H Rogier


    Real-time polymerase chain reaction (PCR) can be considered the gold standard for detection of influenza viruses due to its high sensitivity and specificity. Roche has developed the RealTime ready Influenza A/H1N1 Detection Set, consisting of a generic influenza virus A PCR targeting the M2 gene (M2 PCR) and a specific PCR targeting the hemagglutinin (HA) of A/H1N1-pdm09 (HA PCR, 2009 H1N1), with the intention to make a reliable, rapid, and simple test to detect and quantify 2009 H1N1 in clinical samples. We evaluated this kit against the US Centers for Disease Control and Prevention (USCDC)/World Health Organization real-time PCR for influenza virus using 419 nose and throat swabs from 210 patients collected in 3 large hospitals in Ho Chi Minh City, Vietnam. In the per-patient analysis, when compared to CDC PCR, the sensitivity and specificity of the M2 PCR were 85.8% and 97.6%, respectively; the sensitivity and specificity of HA PCR were 88.2% and 100%, respectively. In the per-sample analysis, the sensitivity and specificity in nose swabs were higher than those in throat swabs for both M2 and HA PCRs. The viral loads as determined with the M2 and HA PCRs correlated well with the Ct values of the CDC PCR. Compared with the CDC PCR, the kit has a reasonable sensitivity and very good specificity for the detection and quantification of influenza A virus and A/H1N1-pdm09. However, given the current status of 2009 H1N1, a kit that can detect all circulating seasonal influenza viruses would be preferable. Copyright © 2012 Elsevier Inc. All rights reserved.

  20. Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene. (United States)

    Abdeldaim, Guma; Svensson, Erik; Blomberg, Jonas; Herrmann, Björn


    A duplex real-time PCR based on the rnpB gene was developed for Mycobacterium spp. The assay was specific for the Mycobacterium tuberculosis complex (MTB) and also detected all 19 tested species of non-tuberculous mycobacteria (NTM). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other non-respiratory body sites. M. tuberculosis was detected by culture in 40 samples and in 30 samples by the assay. The MTB assay showed a sensitivity similar to Roche Cobas Amplicor MTB-PCR (Roche Molecular Systems, Pleasanton, CA, USA). There were only nine samples with non-tuberculous mycobacteria detected by culture. Six of them were detected by the PCR assay. © 2016 APMIS. Published by John Wiley & Sons Ltd.

  1. Regards Croises sur l\\'Alteration Supergene des Roches ...

    African Journals Online (AJOL)

    Les deux modes de caractérisation ou les deux regards croisés sur une même réalité, le premier étant tourné vers l\\'origine et le second vers le devenir, loin de s\\'exclure, se complètent. Mots clés: Altération météorique – Bilans d\\'altération – Altérologie normative – Convergence. (Af. J. of Science and Technology: 2003 ...

  2. Mida roheline tarbija tahab / Catherine Roche ; intervjueerinud Michael S. Hopkins

    Index Scriptorium Estoniae

    Roche, Catherine, 1977-


    2008. a. tehtud uuringust, mille abil selgitati välja, mis põhjustel inimesed eelistavad tarbimisel rohelist tootevalikut. Skeemid: Valmisolek maksta rohelise eest rohkem sõltub tootekategooriast ja tajutavatest eelistest; Teadlikkus ja valik

  3. Nutritionally fastidious Ruminococc$t f lovefociens: strains | Roche ...

    African Journals Online (AJOL)

    The majority of Ruminococcus flavefaciens strains isolated in our laboratories failed to grow or grew poorly both in rumen' fluid medium and in a chemically defined medium containing all the nutrients reported to be required for good growth of this species. ttemptsw ere made to developa defined medium wnicnw ould ...

  4. Nourrir le sol : la roche comme engrais phosphate | CRDI - Centre ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    15 juil. 2011 ... Les paysans qui pratiquent l'agriculture de subsistance en Afrique subsaharienne n'ont pas d'argent de trop à consacrer à l'achat d'engrais. Pourtant, les engrais sont indispensables pour enrichir le sol tropical, pauvre en phosphate, de cette région. Le phosphore favorise l'enracinement précoce, la santé ...

  5. Le souffre thiophénique dans les pétroles et les extraits de roche. Analyse par spectrométrie de masse et chromatographie en phase gazeuse Mass-Spectrometry and Gas-Chromatography Analysis of Thiophene Sulfur in Oil and Rock Extracts

    Directory of Open Access Journals (Sweden)

    Castex H.


    Full Text Available On étudie par spectrométrie de masse à haute résolution la répartition du soufre thiophénique par coupe de distillation et par nombre de carbone dans la fraction > C10 d'un pétrole brut. Parallèlement on chromatographie à l'aide d'un détecteur sélectif à photométrie de flamme les diverses fractions thiophéniques. L'examen des chromatogrammes permet de déterminer les zones dé réponse et l'allure générale des différentes coupes thiophéniques. Les applications sont variées : on vérifie le bien-fondé de l'utilisation d'un spectromètre de masse à basse résolution pour les analyses en routine d'huiles brutes et on peut, par la connaissance des différents profils de type thiophénique prévoir des analogies ou des différences au sein d'huiles brutes et d'extraits de roche d'un même bassin pétrolier, ce qui limite le nombre d'échantillons à analyser en détail. High-resolution mass spectrometry is used to analyze the distribution of thiophenic sulfur per distillation cut and per carbon number in the > C10 fraction of a crude oil. At the same time, a selective flame-photometry sensor is used to chromatograph the different thiophenic fractions. An analysis of the chromatograms serves to determine the response zones and the general aspect of the different thiophenic cuts. There are various applications of this process. The validity of using a low-resolution mass spectrometer is checked for routine crude-cil analyses. By determining the different profiles of the thiophenic type, similarities or differences can be predicted in the crude cils and rock extracts from the saure cil basin. This limits the number of samples having to be analyzed in detail.

  6. Proposition d'explication de la formation d'hydrogène sulfuré dans les stockages souterrains de gaz naturel par réduction des sulfures minéraux de la roche magasin Proposed Explanation of Hydrogen-Sulfide Formation in Underground Natural-Gas Storage Structures by Reduction of Mineral Sulfides in the Reservoir Rock.

    Directory of Open Access Journals (Sweden)

    Bourgeois J. P.


    Full Text Available La formation d'hydrogène sulfuré dans les structures de stockage peu expliquer autrement que par l'action de bactéries sulfato-réductrices. La contenue dans la roche magasin constitue une source de sulfures capable d'alimenter en H2S le gaz naturel. La réduction de la pyrite en sulfures du type Fe 1-x S et l'équilibre de dissolution précipitation, lié principalement à la pression de CO2, dans les structures stockages, constituent un processus de formation d'H2S capable d'expliquer tativement et quantitativement les phénomènes observés sur le terrain. Un modèle simplifié de stockage reprend ce schéma et teste la sensibililté de la teneur en H2S à la valeur des paramètres physiques et chimiques définissant le stockage. Cette étude permet de proposer un certain nombre d'actions susceptibles de limiter la formation d'H2S et d'orienter les choix futurs du couple gaz naturel - structures de stockage. The formation of hydrogen sulfide in storage structures can be explained otherwise thon by the action of sulfate-reducing bacteria. The pyrite contained in the reservoir rock makes up a source of sulfides capable of supplying the natural gas with H2S.Reduction of pyrite ta sulfides of the Fe,-,S type and the dissolution precipitation equilibrium, linked mainly ta C02 pressure in storage structures, make up an H2S for-mation process capable of qualitatively and quantitatively explained phenomena observed in the field.A simplified storage model reflects this scheme and can be used ta test the sensi-tivity of the H2S content ta the value of the physical and chemical parameters defining the storage structure.This investigation can be used to propose various means of action (sable ta "mit H2S formation and ta guide future choices of natural gas/storage-structure pairs.

  7. Quantification and Prediction of the 3D Pore Network Evolution in Carbonate Reservoir Rocks Quantification et prédiction de l’évolution d’un réseau 3D de pores dans des roches réservoir de carbonates

    Directory of Open Access Journals (Sweden)

    De Boever E.


    transport de milieux poreux, résultant des phénomènes de dissolution/cimentation. La validité et prédictibilité de ces modèles dépendent toutefois de la représentativité des réseaux de pores équivalents utilisés et des équations et paramètres utilisés pour modéliser les événements diagénétiques. L’approche développée est appliquée au cas réel d’une roche dolomitique de la formation arabe moyen orientale. La microscopie 2D standard montre que le processus principal affectant la qualité de réservoir consiste en la dolomitisation, suivie d’un renforcement de la porosité dû à une dissolution de la dolomie et à une destruction de la porosité secondaire par cimentation de l’anhydrite diagénétique tardive. La microtomographie par rayons X informatisée (X-rayμ-CT; X-ray computer (microtomography permet de quantifier le volume et la distribution en 3D des différents constituants d’échantillon. Les résultats sont comparés avec les mesures de laboratoire. Des réseaux de pores équivalents avant la dissolution dolomitique et préalablement à la précipitation d’anhydrite tardive sont reconstruits et utilisés pour simuler les caractéristiques de porosité, de perméabilité lors de ces étapes diagénétiques. En utilisant ces structures 3D de pores, la PNM-R (Pore Network Modeling Reactive; modélisation réactive de réseau de pores peut suivre les voies d’évolution plausible de porositéperméabilité entre ces étapes. Les conditions d’écoulement et les vitesses de réaction obtenues par modélisation des voies de réaction géochimiques peuvent être utilisées en tant que référence pour définir les paramètres de modèle de PNM-R. L’approche peut être utilisée pour un typage dynamique de roches et le passage à une échelle supérieure de propriétés pétrophysiques, nécessaires pour la modélisation de réservoir.

  8. Approche numérique et quantitative de l'étude sur clichés aériens de la fracturation des réservoirs en roches fissurées Numerical and Quantitative Approach to Investigating Fracture Maps of Fissured Reservoirs from Airphotos

    Directory of Open Access Journals (Sweden)

    Razack M.


    Full Text Available Dans le cadre de la détermination de la structure des réservoirs en roches fissurées, une méthodologie de traitement numérique et quantitative de la fractu-ration relevée sur clichés aériens a été mise au point. La numérisation du relevé est obtenue par digitalisation sur un lecteur de courbes. Cette information est ensuite traitée à l'aide d'un algorithme (programme RAFRAC qui permet d'identifier numériquement la fracturation par un ensemble de para-mètres élémentaires (orientation, longueur , densité... à partir desquels il devient possible de procéder à une analyse approfondie du champ de fractures considéré. Un exemple d'application est présenté à propos de l'étude de la fracturation d'une zone à structure tabulaire (Causse du Larzac. Ces premiers résultats tendent à mettre en évidence une double nature mathématique de l'information analysée aléatoire d'une part, déterministe d'autre part. On montre également, à partir de la théorie desvariables régionalisées, que la fracturation suit une certaine logique dans sa répartition spatiale. Par ailleurs une organisation des familles de fractures a pu être mise en évidence, sur laquelle la structure géométrique du réservoir sous-jacent s'ajuste de façon très proche. As part of efforts ta determine the structure of reservoirs in fissured rocks, a numerical and quantitative processing methodology has been developed for mapping fractures with airphotos. The survey is digitized on a curve reader. This data is then processed by means of an algorithm (RAFRAC program sa as ta numerically identify fracturing by a set of elementary parameters (direction, length, density, etc. which con be used ta make an in-depth analysis of the fracture field being considered. A sample application is described having ta do with the surveying of the fracturing in a tabular structure (Causse plateau of Larzac. These initial results tend to reveol a dual mathematical nature

  9. Rock Permeability and Fluid Pressure At the Ktb. Implications from Laboratory-And Drill Hole-Measurements Perméabilité des roches et pression dans le KTB : enseignements tirés des mesures de laboratoire et des mesures en puits

    Directory of Open Access Journals (Sweden)

    Zimmermann G.


    Full Text Available Rock permeability and the fluid pressure were investigated at different scales at the two drill holes of the Continental Deep Drilling Program (KTB. Drill hole tests and fluid inclusion investigations both implicate the existence of hydrostatic fluid pressure in situ with respect to salinity of the formation fluid. Matrix permeability and in situ values from hydraulic tests differ up to three decades with higher values in situ. Further on, the pressure dependence of core permeability and in situ determined values differ significantly. All these observed effects support the well known theory of scale variance. This conclusion is supported by observations of hydraulic communications between both drill holes. These scale effects implicate a pronounced hydraulic heterogeneity of the KTB surroundings. Therefore, stochastic network modelling with parameters derived from structural borehole measurements and under the consideration of the observed permeabilities were performed. Under the presumption of existing driving forces fluid transport takes place dominantly on discrete connected pathways characterised by fracture width, fracture length and fracture orientation and is subordinate in the rock matrix. La perméabilité des roches et la pression des fluides ont été étudiées à différentes échelles sur les deux forages du Programme continental de forage profond - Continental Deep Drilling Program (KTB. Les essais de puits et les recherches d'inclusions de fluides impliquent l'existence d'une pression de fluide fonction de la salinité du fluide de formation. Les valeurs déduites des essais de puits dépassent largement les perméabilités matricielles, l'écart allant jusqu'à trois ordres de grandeur. De plus, l'évolution de la perméabilité mesurée sur échantillon en fonction de la pression et celle déterminée in situ diffèrent largement. Ces observations renforcent la théorie bien connue des effets d'échelle. Cette conclusion est

  10. Propagation des ondes élastiques dans les matériaux non linéaires Aperçu des résultats de laboratoire obtenus sur les roches et des applications possibles en géophysique Propagation of Elastic Waves in Nonlinear Materials Survey of Laboratory Results on Rock and Geophysical Applications

    Directory of Open Access Journals (Sweden)

    Rasolofosaon P.


    Full Text Available Les roches présentent souvent un comportement élastique nettement non linéaire, entraînant des conséquences importantes sur la propagation des ondes. Cette non-linéarité élastique est surtout causée par les microdéfauts mécaniques ubiquistes (microfissures, joints de grains, macles, etc. dont la rigidité varie sous l'effet de la contrainte. Ce sujet fait l'objet d'études de plus en plus nombreuses. Nous nous proposons de présenter très sommairement les bases théoriques et les résultats expérimentaux permettant d'avoir un ordre de grandeur des effets caractéristiques observés dans les roches afin de pouvoir proposer une approche critique des possibilités d'applications en géophysique. Deux disciplines se sont développées en parallèle à partir du même principe physique et avec des formalismes très proches : - L'acousto-élasticité étudie l'effet des précontraintes statiques sur les vitesses de propagation des ondes élastiques. On dispose d'un formalisme mécanique élaboré permettant de relier quantitativement variation de contrainte et variation de vitesse élastique (par exemple pour ce qui concerne l'anisotropie acoustique induite par un état de contrainte et d'une méthode expérimentale de mesure des coefficients de non-linéarité. - L'acoustique non linéaire s'intéresse aux conséquences de la variation des modules élastiques au passage d'une onde qui ne peut plus être considérée comme une petite perturbation, mais qui induit localement des modifications mesurables du milieu de propagation ; modifications entraînant l'apparition de phénomènes inconnus en acoustique linéaire tels que la génération d'harmoniques et l'interaction onde-onde. Les applications à la sismique pétrolière semblent fort lointaines puisque, avec les méthodes classiques de surface ou de puits, il y a peu d'espoir de réussir à faire propager jusqu'aux couches profondes des ondes dont l'amplitude dépasserait le seuil de

  11. Global cost modeling analysis of HIV-1 and HCV viral load assays. (United States)

    Elbeik, Tarek; Chen, Yi-Ming Arthur; Soutchkov, Serguei V; Loftus, Richard A; Beringer, Scott


    This review addresses hidden costs associated with the Bayer VERSANT assay, Roche AMPLICOR MONITOR test and COBAS AMPLICOR MONITOR test and how these influence the final per reportable cost to a testing laboratory in resource-rich and -poor countries. An in-depth evaluation and recommendation of the most cost-effective approach for these tests is presented. The analyses demonstrate the need for manufacturers to consider labor and supply costs when marketing a kit in resource-poor countries, noting that marketing strategies need to change. In the absence of any proven monitoring alternative, emphasis is placed on increasing market share to promote significant reduction in kit prices to suit the demands of markets in resource-poor countries. Finally, recommendations are made to improve the overall cost structure of viral load testing. This review is intended as a tool to optimize assay usage in attaining the lowest performance costs by assay and is not to endorse any test, as will become apparent.

  12. Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.

    LENUS (Irish Health Repository)

    Levis, J


    BACKGROUND: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. OBJECTIVE: To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. METHODOLOGY: Viral load quantification of each patient\\'s first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization, using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. RESULTS: Retrospective evaluation of first quantitative values suggested that 82.4% (n=168\\/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per ml. A few patients (3.4%; n=7\\/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n=33\\/54). CONCLUSION: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification.

  13. The Usefulness of Defining Rapid Virological Response by a Very Sensitive Assay (TMA) during Treatment of HCV Genotype 2/3 Infection

    DEFF Research Database (Denmark)

    Dalgard, Olav; Martinot-Peignoux, Michelle; Verbaan, Hans


    The aim of this study was to determine in patients with HCV genotype 2 or 3 the performance at week 4 of two assays with different sensitivities for HCV RNA detection, for the prediction of SVR and stratification for treatment duration (14 and 24 weeks). Recruitment was from two trials comparing 14...... and 24 weeks treatment to patients with rapid virological response (RVR) (n = 550). RVR was originally defined as HCV RNA HCV-RNA was prospectively...... measured with COBAS Amplicor V2, Roche (CA) (lower detection limit 50 IU/ml) and retrospectively assessed with VERSANT HCV-RNA Qualitative Assay, Siemens (TMA) (lower limit detection 10 IU/ml). Genotype 3 was present in 80% and genotype 2 in 20%. A SVR was achieved in 82%. At week 4 HCV...

  14. Roche genome sequencer FLX based high-throughput sequencing of ancient DNA

    DEFF Research Database (Denmark)

    Alquezar-Planas, David E; Fordyce, Sarah Louise


    Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure tha...

  15. Supersymmetric Proof of the Hirzebruch-Riemann-Roch Theorem for Non-Kähler Manifolds

    Directory of Open Access Journals (Sweden)

    Andrei V. Smilga


    Full Text Available We present the proof of the HRR theorem for a generic complex compact manifold by evaluating the functional integral for the Witten index of the appropriate supersymmetric quantum mechanical system.

  16. H08117: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1959-04-28 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  17. H08115: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-06-29 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  18. H08116: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-09-13 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  19. Defending the Freedom to Innovate: Faculty Intellectual Property Rights after "Stanford v. Roche" (United States)

    American Association of University Professors, 2014


    Tensions over faculty control of the fruits of their scholarship have been slowly building since the 1980s, but they have also intensified since late 2011. There have long been differences of opinion over ownership of patentable inventions, but over the last two years a number of universities have categorically asserted that they own these…

  20. On the Use of Roche Equipotentials in Analysing the Problems of ...

    Indian Academy of Sciences (India)


    Jan 27, 2016 ... Since January 2016, the Journal of Astrophysics and Astronomy has moved to Continuous Article Publishing (CAP) mode. This means that each accepted article is being published immediately online with DOI and article citation ID with starting page number 1. Articles are also visible in Web of Science ...

  1. H08116A: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-09-13 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  2. Clinic-based evaluation of Clearview Chlamydia MF for detection of Chlamydia trachomatis in vaginal and cervical specimens from women at high risk in China. (United States)

    Yin, Y-P; Peeling, R W; Chen, X-S; Gong, K-L; Zhou, H; Gu, W-M; Zheng, H-P; Wang, Z-S; Yong, G; Cao, W-L; Shi, M-Q; Wei, W-H; Dai, X-Q; Gao, X; Chen, Q; Mabey, D


    To determine the performance of a rapid Chlamydia trachomatis (CT) test (Clearview Chlamydia MF) compared to the current "gold standard" (Roche Amplicor CT assay) test, and to assess acceptability of the tests to patients. A total of 1497 women at sexually transmitted diseases (STD) clinics or re-education centres in six urban cities (Shanghai, Nanjing, Shenzhen, Guangzhou, Chengdu and Fuzhou) in China participated in the study. Three vaginal and three cervical swabs were collected from each participant. Rapid CT tests were performed locally on the first vaginal and cervical swabs and the results were read independently by two staff members. The second and third swabs were randomised for performing the Roche CT assay at the National STD Reference Laboratory. Acceptability of the rapid tests to patients was determined by asking patients in clinics about their willingness to wait for the results. The prevalence of CT was 13.2% (197/1497), as determined by the Roche assay with cervical specimens. CT was detected in 78 vaginal and 127 cervical specimens by the rapid test and the positive rates determined with cervical specimens were significantly higher than those with vaginal specimens (pgood agreement between the results read by two independent staff for either vaginal or cervical specimens (both kappa = 0.98, p<0.001). Sensitivities for vaginal and cervical specimens were 32.8% and 49.7%, respectively, and specificities were 99.2% and 97.9%, respectively. The positive predictive value was 85.7% for vaginal and 78.4% for cervical specimens. The vast majority of the patients (99.1%) were willing to wait up to two hours for the results. Clearview Chlamydia MF, while yielding a rapid result and requiring minimal laboratory facilities, had unacceptably low sensitivity compared to a nucleic acid amplification test. Rapid tests yielding results within one hour are generally accepted by the clients.

  3. Genotype 3 is the predominant hepatitis C genotype in a multi-ethnic Asian population in Malaysia. (United States)

    Ho, Shiaw-Hooi; Ng, Kee-Peng; Kaur, Harvinder; Goh, Khean-Lee


    Genotypes of hepatitis C virus (HCV) are distributed differently across the world. There is a paucity of such data in a multi-ethnic Asian population like Malaysia. The objectives of this study were to determine the distribution of HCV genotypes between major ethnic groups and to ascertain their association with basic demographic variables like age and gender. This was a cross-sectional prospective study conducted from September 2007 to September 2013. Consecutive patients who were detected to have anti-HCV antibodies in the University of Malaya Medical Centre were included and tested for the presence of HCV RNA using Roche Cobas Amplicor Analyzer and HCV genotype using Roche single Linear Array HCV Genotyping strip. Five hundred and ninety-six subjects were found to have positive anti-HCV antibodies during this period of time. However, only 396 (66.4%) were HCV RNA positive and included in the final analysis. Our results showed that HCV genotype 3 was the predominant genotype with overall frequency of 61.9% followed by genotypes 1 (35.9%), 2 (1.8%) and 6 (0.5%). There was a slightly higher prevalence of HCV genotype 3 among the Malays when compared to the Chinese (P=0.043). No other statistical significant differences were observed in the distribution of HCV genotypes among the major ethnic groups. There was also no association between the predominant genotypes and basic demographic variables. In a multi-ethnic Asian society in Malaysia, genotype 3 is the predominant genotype among all the major ethnic groups with genotype 1 as the second commonest genotype. Both genotypes 2 and 6 are uncommon. Neither genotype 4 nor 5 was detected. There is no identification of HCV genotype according to ethnic origin, age and gender.

  4. Stable hepatitis C virus RNA detection by RT-PCR during four days storage

    Directory of Open Access Journals (Sweden)

    Horsmans Yves


    Full Text Available Abstract Background Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4°C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples. Methods Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 – 25.4°C or at 4°C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems. Results The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4°C. Conclusions We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4°C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.

  5. Evaluation of the Hologic gen-probe PANTHER, APTIMA Combo 2 assay in a tertiary care teaching hospital. (United States)

    Cheng, Annie; Kirby, James E


    To evaluate the performance of the Hologic Gen-Probe (San Diego, CA) PANTHER system. The performance of PANTHER was compared with the Hologic Gen-Probe TIGRIS and/or Roche (Indianapolis, IN) COBAS AMPLICOR systems through testing of patient specimens and the spiked-urine matrix. After discrepant resolution, PANTHER demonstrated a 99.3% (95% confidence interval [CI], 96.0%-99.9%) positive and 100% (98.5%-100.0%) negative agreement for Chlamydia trachomatis (CT) and 100% (96.6%-100.0%) positive and 100% (98.6%-100.0%) negative agreement for Neisseria gonorrhoeae (NG) for all male, female, unsexed, and NG-spiked female urine specimens combined. For other specimen types collectively, the PANTHER demonstrated 100% (95% CI, 90.6%-100.0%) positive and 100% (88.3%-100.0%) negative agreement for CT and 90.9% (62.8%-98.4%) positive and 100% (93.5%-100.0%) negative agreement for NG. Analytical sensitivity of the PANTHER in urine matrix was similar to the TIGRIS system. The PANTHER system provides an excellent new addition to options for detecting CT and NG, is appropriate for testing urine samples, and will facilitate high-throughput testing in the clinical laboratory.

  6. Persistence of hepatitis C virus in a white population: associations with human leukocyte antigen class 1.

    LENUS (Irish Health Repository)

    Fanning, Liam J


    The aim of this study was to define novel associations between human leukocyte antigen (HLA) class 1 alleles and persistence or clearance of the hepatitis C virus (HCV) in a white population. All individuals in the study were seropositive for anti-HCV antibodies. Viral status was determined by the Roche HCV Amplicor test. HLA-A, -B, -C allelic group profile was molecularly defined by reverse line probe hybridization. The strongest individual allelic group associations with persistent HCV infection were HLA A*11 (p = 0.044) and Cw*04 (p = 0.006). However, only the HLA C*04 association survived correction for multiple comparisons. Further analysis of alleles in linkage with HLA Cw*04 revealed that the haplotype HLA A*11, Cw*04 was present in 11 individuals, 10 of whom were viremic (p = 0.05). No gene dosage effect was observed. No association between HLA class 1 allelic groups and aviremia and virus load was evident in this white population. HLA B*44 is associated with low virus load in human immunodeficiency virus disease, but this association was not evident in this HCV-infected population. Novel HLA class 1 alleles associated with persistence of HCV have been identified.

  7. Sieroprevalenza di infezione da HBV e HCV tra pazienti in dialisi

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    Rosa Anna Leone


    Full Text Available The aim of the present study was to investigate the seroprevalence of HBV and HCV among dialysis patients in the Lamezia Terme (CZ area during the period 1999-2002. Sera from 63 patients in haemodialysis (HD and 10 patients in peritoneal dialysis (PD were analyzed with a follow-up every three months for HBsAg, HBcAb, HBsAb, anti-HCV and anti-HIV (Elisa Test,AxSYM,Abbott;we analyzed reactive sera for anti-HCV by using supplemental test (RIBA Test, Ortho; we also looked for viremia (RT-PCR Amplicor, Roche Diagnostics and HCV genotypes (Inno-Lipa HCV II, Innogenetics.The results show that, among the HD patients, 3 were HBsAg positive (Chronic Infection and 7 HBcAb and HBsAb positive/HBsAg negative (Passed Infection; 14 individuals were anti-HCV positive. No patients in PD were positive for HBV and HCV markers.The prevalence of chronic HBV infection was 4.8% (instead of 3% in other Dialysis Units, that of anti-HCV positive was 22% (in others 24%- 33%; among anti-HCV positive patients, the HCV-RNA prevalence was 79% (instead of 80%; the most recurrent HCV genotype was 2a/2c (instead of 1b in general population.These findings lead us to hypothesize that the environmental transmission in the dialysis setting is tightly correlated to the risk of HBV and HCV infection.

  8. Les roches basiques et ultrabasiques des Lacs Robert et le Trias de Chamrousse (Massif de Belledonne) Etude petrologique et geologique

    NARCIS (Netherlands)

    Tex, den E.


    La région que j’ai étudiée et relevée est située dans les Alpes françaises, à environ 4° longitude est et 50°15’ latitude nord, et à environ 2000 mètres d’altitude. Elle fait partie de la chaîne dite „chaîne de Belledonne” qui s’étend des massifs de Beaufort et du Grand Mont dans le N N E jusqu’à

  9. Les types des roches basiques et ultrabasiques qu’on rencontre dans la partie occidentale de la Galice (Espagne)

    NARCIS (Netherlands)

    Koning, H.


    A description is given of the arrangement cf the mafic and ultramafic rocks within the metamorphic complex in Galicia (NW Spain), followed by a brief petrographic description of some frequently found metamorphic types. Differences in metamorphic state and field relations lead to the conclusion, that

  10. High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform

    DEFF Research Database (Denmark)

    Fordyce, Sarah Louise; Avila Arcos, Maria del Carmen; Rockenbauer, Eszter


    The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR rep...

  11. A comparison of rumen microbial profiles in dairy cows as retrieved by 454 Roche and Ion Torrent (PGM sequencing platforms

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    Nagaraju Indugu


    Full Text Available Next generation sequencing (NGS technology is a widely accepted tool used by microbial ecologists to explore complex microbial communities in different ecosystems. As new NGS platforms continue to become available, it becomes imperative to compare data obtained from different platforms and analyze their effect on microbial community structure. In the present study, we compared sequencing data from both the 454 and Ion Torrent (PGM platforms on the same DNA samples obtained from the rumen of dairy cows during their transition period. Despite the substantial difference in the number of reads, error rate and length of reads among both platforms, we identified similar community composition between the two data sets. Procrustes analysis revealed similar correlations (M2 = 0.319; P = 0.001 in the microbial community composition between the two platforms. Both platforms revealed the abundance of the same bacterial phyla which were Bacteroidetes and Firmicutes; however, PGM recovered an additional four phyla. Comparisons made at the genus level by each platforms revealed differences in only a few genera such as Prevotella, Ruminococcus, Succiniclasticum and Treponema (p < 0.05; chi square test. Collectively, we conclude that the output generated from PGM and 454 yielded concurrent results, provided stringent bioinformatics pipelines are employed.

  12. Cytology and Human Papillomavirus Testing 6 to 12 Months after ASCUS or LSIL Cytology in Organized Screening To Predict High-Grade Cervical Neoplasia between Screening Rounds (United States)

    Sjøborg, Katrine D.; Nygård, Mari; Røysland, Kjetil; Campbell, Suzanne; Alfsen, G. Cecilie; Jonassen, Christine M.


    We carried out a prospective study comparing the performance of human papillomavirus (HPV) E6/E7 mRNA (PreTect HPV-Proofer; NorChip, Klokkarstua, Norway) and DNA (Amplicor HPV test; Roche Diagnostics, Basel, Switzerland) triage testing of women 6 to 12 months after atypical-squamous-cells-of-undetermined-significance (ASCUS) or low-grade-squamous-intraepithelial-lesion (LSIL) cytology in organized screening to predict high-grade cervical intraepithelial neoplasia of grade 2 or worse (CIN2+) between screening rounds. Between January 2005 and April 2008, 692 study women with screening-detected ASCUS/LSIL cytology 6 to 12 months earlier returned for HPV mRNA and DNA testing and repeat cytology. The median follow-up time was 3 years, using existing health care facilities. Follow-up test results were available for 625 women. Of the 145 CIN2+ cases detected during the study period, 95 (65.5%) were HPV mRNA positive 6 to 12 months after screening-detected ASCUS/LSIL, 44 (30.4%) were HPV mRNA negative, and 6 (4.1%) were invalid. The corresponding HPV DNA results were 139 (95.9%), 5 (3.4%), and 1 (0.7%), respectively. The cumulative incidences of CIN2+ 3 years after a negative HPV mRNA and DNA test were 10.3% (95% confidence interval [CI], 7.2 to 13.3%) and 1.8% (95% CI, 0.0 to 3.6%), respectively. The cumulative incidences of CIN2+ 3 years after positive HPV mRNA and DNA tests were 52.8% (95% CI, 40.1 to 60.1%) and 41.3% (95% CI, 35.5 to 46.6%), respectively. In conclusion, both positive HPV mRNA and DNA test results have a high enough long-term prediction of CIN2+ risk to consider referral to colposcopy as good practice when performed in delayed triage of women with ASCUS/LSIL cytology. In addition, the low CIN2+ risk among women with a negative Amplicor HPV test in our study confirms its safe use in a clinical setting. PMID:22518869

  13. Nucleic acid amplification technology screening for hepatitis C virus and human immunodeficiency virus for blood donations

    International Nuclear Information System (INIS)

    Bamaga, Mohammad S.; Bokhari, Fawzi F.; Aboud, Abdulrehman M.; Al-Malki, M.; Alenzi, Faris Q.


    To investigate the performance of the commercial Roche COBAS AmpliScreen assay, and demonstrate whether the COBAS AmpliScreen human immunodeficiency virus-1 (HIV-1) test, v1.5, and COBAS AmpliScreen hepatitis C virus (HCV) v 2.0 for screening for HIV-1 and HCV RNA in the donated blood units from which plasma mini pools were collected, by nucleic acid amplification technology (NAT), could detect the positive pools and reduce the risk of transmission of infections for those routinely tested by serological assays. The study was performed on 3288 plasma samples collected from blood donors in a period of 13 months, from August 2004 to August 2005, at Al-Hada Armed Forces Hospital, Molecular Pathology Laboratory, Taif, Kingdom of Saudi Arabia. The samples were tested by the reverse transcriptase polymerase chain reaction (RT-PCR) after RNA extraction (this represents the major method in NAT assays), in parallel with the routine serological testing to detect qualitatively for HIV-1 and HCV. The NAT assays that include an automated COBAS AmpliPrep system for RNA extraction and COBAS Amplicor Analyzer using AmpliScreen kits for RT-PCR assays, and the routine serological screening assays for the detection of the HIV-1 and HCV RNA in the plasma samples from the blood donors have shown to be a reliable combination that would meet our requirements. The collected data further confirms the results from the serological assays and enables us to decrease the residual risk of transmission to a minimum with the finding of no seronegative window period donation. The results demonstrate that out of 3288 samples, the percentages of RT-PCR (NAT) negative blood donations that were also confirmed as seronegative were 99% for HCV, and 99.1% for HIV-1. The modified combined systems (automated COBAS AmpliPrep system for RNA extraction and COBAS Amplicor Analyzer using AmpliScreen kits for RT-PCR assays) for NAT screening assays has allowed the release of all blood donations supplied in the

  14. Treatment seeking behaviors related to gonorrhea among female sex workers in 7 cities in Indonesia

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    Roselinda Roselinda


    Full Text Available Abstrak Latar belakang:Gonore merupakan salah satu infeksi menular seksual yang menjadi permasalahan besar kesehatan terutama pada wanita penjaja seks (WPS di Indonesia. Tujuan dari artikel ini adalah untuk melihat hubungan antara pola pencarian pengobatan gonore. Metode:Data berasal dari studi potong lintang dengan responden WPS yang dipilih secara cluster random sampling dari 7 kota (Timika, Yogyakarta, Kupang, Samarinda, Pontianak, Makassar dan Tangerang di Indonesia pada tahun 2007. Diagnosis gonore berdasarkan hasil pemeriksaan Polymerase Chain Reaction (PCR menggunakan Amplicor CT/NG dari Roche yang telah disetujui oleh World Health Orgazation (WHO sebagai alat skrining gonore. Hasil:Proporsi responden yang menderita gonore sebesar 26.1% (404/1750. Persentase penderita gonore yang melakukan upaya pengobatan terdistribusi hampir sama dengan yang mengunjungi fasilitas kesehatan / dokter dengan yang membeli obat sendiri. Subyek yeng melakukan pengobatan tradisional memiliki risiko 44% lebih tinggi menderita gonore dibandingkan dengan subyek yang melakukan pengobatan di fasilitas kesehatan / dokter [risiko relatif suaian (RRa = 1,44; P = 0.044]. Sedangkan subyek yang tidak diobati dibandingkan dengan yang berobat ke fasilitas kesehatan / dokter lebih berisiko 55% menderita gonore (RRa = 1.55; P = 0.002.Kesimpulan: Wanita penjaja seks yang melakukan maupun yang tidak pengobatan tradisional dibandingkan dengan yang mengunjungi fasilitas kesehatan/dokter memiliki risiko yang lebih tinggi menderita gonore. (Health Science Indones 2013;2:87-92Kata kunci:gonore, wanita penjaja seks, IndonesiaAbstractBackground:Gonorrhea is one of sexually transmitted infections that have become a major health problem especially among female sex workers (FSW in Indonesia. The objective of this article is to identify the relationship between treatment seeking behaviors, the sites of study and gonorrhea among FSW. Methods: The data that analyzed derived from cross

  15. Chlamydia trachomatis infection and associated risk factors in a low-income marginalized urban population in coastal Peru Infección por Chlamydia trachomatis y factores de riesgo asociados en una población marginal urbana de bajos ingresos de la costa peruana

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    Segundo R. León


    Full Text Available OBJECTIVES: To estimate Chlamydia trachomatis (CT infection prevalence and associated risk factors among a low-income marginalized urban population in Peru. METHODS: Between April 2003 and April 2005, men and women at high-risk for sexually transmitted infections (STIs were recruited from low-income urban areas in three coastal cities in Peru (Chiclayo, Lima, and Trujillo. Consenting participants were studied using a sero-epidemiologic survey. Urine and vaginal swabs collected from men and women were evaluated using polymerase chain reaction (PCR (COBAS® AMPLICOR (CT/NG Test, Roche Molecular Diagnostics, Branchburg, NJ, USA for CT. RESULTS: Among the 2 440 participants recruited for the study (2 145 men and 295 women, overall prevalence of CT infection was 6.6% (95% CI, 5.6-7.6%: 5.5% (95% CI, 4.5-6.5% in men and 14.9% (95% CI, 11.7-27.1% in women. Chlamydial infection was inversely associated with age and positively associated with HIV infection and dysuria in men. Among women, chlamydial infection was inversely associated with age and positively associated with number of sex partners. CONCLUSIONS: CT infection was common among high-risk men and women in urban coastal Peru. Because chlamydial infection is associated with complications related to female reproduction, including infertility and ectopic pregnancy, interventions to prevent and treat infection and studies to determine the feasibility of population-based screening for CT should be conducted among the high-risk female population.OBJETIVOS: Estimar la prevalencia de la infección por Chlamydia trachomatis y los factores de riesgo asociados en una población marginal urbana de bajos ingresos de Perú. MÉTODOS: Entre abril de 2003 y abril de 2005 se captaron hombres y mujeres con alto riesgo de contraer infecciones de transmisión sexual, en áreas urbanas de bajos ingresos de tres ciudades costeras de Perú (Chiclayo, Lima y Trujillo. Los participantes que aceptaron colaborar

  16. [Innate immunity restoration in patients with HIV/AIDS infection associated with antiretroviral therapy]. (United States)

    Afani, Alejandro; Jiusán, Lorena; Raby, Pablo; Sitia, Giovanni; Puente, Javier; Sepúlveda, Cecilia; Miranda, Dante; Cabrera, Roy; Guidotti, Luca; Lanza, Paola


    Highly active antiretroviral therapy (HAART) in HIV/AIDS infection induces an important reduction of the viral load (VL) and an immune system reconstitution. CD4+ T lymphocyte count is the immunological measurement commonly used for the follow up of HIV/AIDS patients. To study prospectively the restoration of the innate immune system in patients with HIV/AIDS infection during their first year on HAART. 25 naive HIV/AIDS patients, from San José Hospital and University of Chile Clinical Hospital, Santiago, Chile, were studied between years 2002-2003. Every 4 months after HAART initiation, CD3+, CD4+, CD8+ T lymphocytes and CD16/56+ natural killer (NK) cells were quantified by flow cytometry. NK cell cytotoxicity was measured using radioactive chrome liberation (Cr51). Tumor necrosis factor alpha (TNF-alpha) and interleukin-10 (IL-10) were measured in peripheral blood mononuclear cells and viral load was determined using Amplicor HIV-1 from Roche Diagnostics Systems. Thirteen of the 25 patients continued in the study. They were all males, average age 35 years old (23-50). At baseline average CD4+ count was 146 cells/microL (31-362) and average viral load was 82.000 copies/mL (4.000-290.000). A raise in CD3+, CD4+, CD8+, and CD16/56 cells was noted at months 9-12 of therapy. Viral load became undetectable in the same period. NK cell function was decreased at the beginning of the therapy (1-4 months), reaching its highest values at months 9-12. There was no significant change in IL-10. TNF-alpha increased in six patients during the study. In this group of patients, innate immunity was restored during HAART. These results should be confirmed in studies with a longer follow up period and also measuring cytokines such as MIP-1a, MIP-1ss and RANTES.

  17. Prevalence of oral lesions in HIV patients related to CD4 cell count and viral load in a Venezuelan population. (United States)

    Bravo, Inés María; Correnti, María; Escalona, Laura; Perrone, Marianella; Brito, Aubert; Tovar, Vilma; Rivera, Helen


    To determine the prevalence of oral lesions in a HIV+ group of patients, related to CD4 cell count and viral load in a Venezuelan population. In the present study, we evaluated 75 HIV+ adult patients, attended at the Center of Infectious Diseases, at the Faculty of Dentistry, Central University of Venezuela. Each patient was clinically examined for detection of oral mucosal lesions. In addition, CD4 cell count was determined by flow cytometry, as well as viral load by RT-PCR (Amplicor HIV-RNA, TM test 1.5, Roche). 85% (64/75) of HIV/AIDS patients showed associated HIV lesions. Oral Candidiasis constituted the most common lesion representing a 61% (39/64), followed by Oral Hairy Leukoplakia 53% (34/64); Oral Leukoplakia 34% (22/64), Melanic Hyperpigmentation 38% (18/64); Papilloma 13 (6/64), Lineal Gingival Erythema 8% (5/64); Aphtous Recurrent Stomatitis 5% (4/64) and Kaposi's Sarcoma 5% (3/64). Only one case of the following lesions were represented by Non Hodgkin Lymphoma, Multifocal Epithelial Hyperplasia, Recurrent Herpes, Histoplasmosis and Molluscum Contagiosum. The patients with a viral load of 30.000 copies/mm3 exhibited oral lesions related with HIV, independent of CD4 cell count, although patients with CD4+ levels of 200 cel/mm3 were more susceptible to develop these lesions. The most common oral lesion was Oral Candidiasis followed by Oral Hairy Leukoplakia, Oral Leukoplakia and Melanic Hyperpigmentation. A high viral load was strongly associated to the oral lesions occurrence independently of CD4+ cell count.

  18. Expression of HLA-G1 at the placental interface of HIV-1 infected pregnant women and vertical transmission of HIV. (United States)

    Moodley, S; Bobat, R


    The ability of Human Leucocyte Antigen-G (HLA-G) to inhibit the cytolytic effect to immunocompetent cell types, suggests that HLA-G has an immunomodulatory role. In view of this concept the objective of the study was to assess whether the Major Histocompatibility Complex -coded molecule HLA-G mRNA is a risk factor at the placental barrier in HIV-1 positive pregnant women. Placental HLA-G1 levels in HIV-1 infected mothers and viral loads in both mothers and their babies were performed on fifty-five participants. Synthesis of complementary deoxyribose nucleic acid (cDNA) was performed using ribose nucleic acid (RNA) extracted from placental tissue samples. Amplification of cDNA using specifically designed primers complementary to the full length HLA-G1 isoform was quantified using real time-polymerase chain reaction (RT-PCR). Viral load assays (Amplicor Version 1.5, Roche Diagnostics) were performed on all plasma samples. HLA-G1 primers detected the full length isoform HLA-G1 PCR product at 86.5 °C. Logistic regression calculations indicated that the risk of babies becoming infected increased by 1.3 with every 1 unit increase in HLA-G1 expression. Female babies were 3.7 times more likely to become infected than male. There was a positive correlation between mothers' log viral load and transmission of infection to the baby (p = 0.047; 95%CI 1.029-11.499). Maternal viral load was a strong predictor of viral transmission. Placental HLA-G1 expression was up-regulated 3.95 times more in placentas of HIV-1 infected mothers with infected babies when compared to uninfected babies. Copyright © 2011 Elsevier Ltd. All rights reserved.

  19. Indeterminate RIBA results were associated with the absence of hepatitis C virus RNA (HCV-RNA in blood donors

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    Felicidade Mota Pereira


    Full Text Available Introduction: Hepatitis C virus (HCV infection is diagnosed by the presence of antibodies and is supplemented by confirmatory testing methods, such as recombinant immunoblot assay (RIBA and HCV-RNA detection. This study aimed to evaluate the efficacy of RIBA testing to diagnose HCV infection in blood donors positive for anti-HCV antibodies. Methods: A total of 102 subjects positive for anti-HCV determined by enzyme-linked immunosorbent assay (ELISA at the Hematology and Hemotherapy Foundation of Bahia (HEMOBA were later assessed with new samples using the Abbott Architect anti-HCV test (Abbott Diagnostics, Wiesbaden, Germany, the RIBA III test (Chiron RIBA HCV 3.0 SIA, Chiron Corp., Emeryville, CA, USA, the polymerase chain reaction (PCR; COBAS® AMPLICOR HCV Roche Diagnostics Corp., Indianapolis, IN, USA and line probe assay (LiPA - Siemens, Tarrytown, NY, USA genotyping for HCV diagnosis. Results: Of these new samples, 38.2% (39/102 were positive, 57.8% (59/102 were negative and 3.9% (4/102 were indeterminate for anti-HCV; HCV-RNA was detected in 22.5% (23/102 of the samples. RIBA results were positive in 58.1% (25/43, negative in 9.3% (4/43 and indeterminate in 32.6% (14/43 of the samples. The prevailing genotypes were 1 (78.3%, 18/23, 3 (17.4%, 4/23 and 2 (4.3%, 1/23. All 14 samples with indeterminate RIBA results had undetectable viral loads (detection limit ≤50 IU/mL. Of these samples, 71.4% (10/14 were reevaluated six months later. Eighty percent (8/10 of these samples remained indeterminate by RIBA, and 20% (2/10 were negative. Conclusions: In this study, individuals with indeterminate RIBA results had no detectable HCV-RNA.

  20. Comparison of M4 and M4RT media for transporting cervical swab samples for PCR detection of Chlamydia trachomatis and Neisseria gonorrhoeae. (United States)

    Aslanzadeh, Jaber; Jones, Mathew


    In a prospective study, M4RT medium was compared to the traditional M4 medium to transport cervical swab specimens for Neisseria gonorrhoeae/Chlamydia trachomatis (NG/CT) PCR testing using the Roche COBAS Amplicor. Two cervical swab samples were collected from 270 consecutive patients screened for NG/CT in a satellite facility. The swabs were placed individually in M4RT and M4 medium and were immediately refrigerated, transported to the laboratory on wet ice, and stored at 2 to 8 degrees C until the PCR testing was performed within 7 da of collection. Seven of the cervical swab samples transported in M4 or M4RT were PCR positive for CT. Two additional samples transported in M4RT and a third swab transported in M4 were CT PCR positive. These samples were PCR negative in the alternative medium. Similarly, 12 of the cervical swabs transported in M4 or M4RT were NG PCR positive. Three additional swabs transported in M4 media were NG PCR positive. Initially, 2 of these samples when transported in M4RT were NG PCR equivocal and were considered NG PCR positive on repeat testing. Similarly, 2 additional swab samples transported in M4 RT media were NG PCR positive. These samples, when transported in M4 media, were NG PCR equivocal or negative. However, on repeat testing the equivocal sample was considered NG PCR positive. We conclude M4 and M4RT transport media are equally reliable for transporting cervical swab samples for NG/CT PCR testing. M4RT medium is more convenient to use, as it did not require refrigeration until it was inoculated with the clinical sample.

  1. Prevalence of Sexually Transmitted Infection in Teenage Pregnancy in Rajavithi Hospital, Thailand. (United States)

    Asavapiriyanont, Suvanna; Chaovarindr, Udom; Kaoien, Surasak; Chotigeat, Uraiwan; Kovavisarach, Ekachai


    Behavioral and social changes in the modern era have triggered an increase in the incidence of early sexual contact and teenage pregnancy. Since there is no routine Gonococcal & Chlamydial (GC & CT) screening in teens in antenatal clinics in Thailand, the present study was performed to find the prevalence of STI, especially Chlamydial infection, in teenage pregnancy. To evaluate the prevalence of sexually transmitted infections (STIs), especially Chlamydial infection (CT), in teenage pregnancy and its related factors. One hundred and twenty-one teenage pregnancies were recruited at the ANC in Rajavithi Hospital from October 2006 to May 2007. After signing informed consent forms, they were asked to answer questionnaires about baseline data, sexual information and risk factors, after which urine specimens were collected for screening for GC and CT using the PCR technique (AMPLICOR by Roche). Later, pelvic examination was per formed by the gynecologist at the STD (sexually transmitted disease) clinic. All the data and LAB results were recorded and analyzed by the SPSS program. Numbers, percentages, means with SD, Chi-squared test, Fisher's exact test and odds ratio were used. Potential risk factors were analyzed using binary logistic regression. The prevalence of STI in pregnant teenagers was 28.1% (CT = 19.8%, GC = 1.7%, hepatitis B = 3.3%, trichomoniasis 1.7%, Herpes simplex = 0.8% and condyloma acuminata = 0.8%). No Syphilis, chancroid or HIV were found in the present study Other non-STI like candidiasis and bacterial vaginosis were found in 45.5% of participants (candidiasis and bacterial vaginosis at 19.0% and 24.8%, respectively). The risk of CT infection was significantly related (6.9 times higher) to having previous sexual contact before the current partner (95% CI, 1.8-27.0). STI, especially Chlamydial infection, was found in a significant number of teenage pregnancies. Measures should be taken to prevent this resulting in complicated outcomes in the future.

  2. Abbott RealTime PCR assay is useful for evaluating virological response to antiviral treatment for chronic hepatitis C. (United States)

    Ikezaki, Hiroaki; Furusyo, Norihiro; Ihara, Takeshi; Hayashi, Takeo; Ogawa, Eiichi; Toyoda, Kazuhiro; Taniai, Hiroaki; Kainuma, Mosaburo; Murata, Masayuki; Hayashi, Jun


    This study was done to evaluate the utility of the Abbott RealTime PCR assay (ART) for the monitoring of chronic hepatitis C patients. The serum samples of 183 patients infected with hepatitis C virus (HCV) genotype 1b who had completed a 48-week period of pegylated interferon (PEG-IFN) alpha-2b plus ribavirin treatment were prospectively analyzed. Serum HCV RNA levels were measured both by ART and by the Roche COBAS Amplicor Monitor test, version2.0 (CAM) at baseline and at weeks 4, 12, 24, 36, and 48 of treatment, and at 24 weeks after the end of treatment (EOT). A significant positive correlation of pretreatment HCV RNA levels was found between ART and CAM (r = 0.595, P HCV RNA level from the pretreatment level determined by ART in SVR patients was significantly higher than that in non-SVR patients at all time points tested. The logarithmic decline determined by CAM in SVR patients was significantly higher than that in non-SVR patients only at week 4, but there was no significant difference at other weeks. Of 124 patients who were HCV RNA-negative at EOT by ART, 58 (46.8%) had a relapse of viremia at 24 weeks after EOT, whereas 77 of 143 patients (53.8%) who were HCV RNA-negative at EOT by CAM had a relapse. The relapse rate was lower when determined by ART than by CAM, but not significantly so. ART is more useful than CAM for evaluating the virological response to antiviral treatment for chronic hepatitis C.

  3. Chlamydia trachomatis infection and sexual behaviour among female students attending higher education in the Republic of Ireland.

    LENUS (Irish Health Repository)

    O'Connell, Emer


    BACKGROUND: There are no prevalence data on Chlamydia trachomatis relating to female students attending higher education available for the Republic of Ireland. This information is required to guide on the necessity for Chlamydia screening programmes in higher education settings. This research aimed to determine the prevalence of and predictive risk factors for Chlamydia trachomatis genital infection among female higher education students in Ireland. METHODS: All females presenting during one-day periods at Student Health Units in three higher education institutions in two cities in the Republic of Ireland were invited to participate. Participants completed a questionnaire on lifestyle and socio-demographic factors and provided a urine sample. Samples were tested for C. trachomatis DNA by a PCR based technique (Cobas Amplicor, Roche). To examine possible associations between a positive test and demographic and lifestyle risk factors, a univariate analysis was performed. All associations with a p value < 0.05 were included in a multivariate logistic regression analysis. RESULTS: Of the 460 sexually active participants 22 tested positive (prevalence 4.8%; 95% CI 3.0 to 7.1%). Variables associated with significantly increased risk were current suggestive symptoms, two or more one-night stands and three or more lifetime sexual partners. The students displayed high-risk sexual behaviour. CONCLUSION: The prevalence of C. trachomatis infection and the lack of awareness of the significance of suggestive symptoms among sexually experienced female students demonstrate the need for a programme to test asymptomatic or non-presenting higher education students. The risk factors identified by multivariate analysis may be useful in identifying those who are most likely to benefit from screening. Alcohol abuse, condom use, sexual behaviour (at home and abroad) and, knowledge of sexually transmitted infections (STIs) (including asymptomatic nature or relevant symptoms) were

  4. “Knaller-Sex für alle”: Popfeminist Body Politics in Lady Bitch Ray, Charlotte Roche, and Sarah Kuttner

    Directory of Open Access Journals (Sweden)

    Carrie Smith-Prei


    Full Text Available Germany has seen a recent upsurge in publications proclaiming that feminism is again an urgent matter for a new generation of women. Faced with the reactionary demography debate and the hegemony of second-wave feminism, young writers, musicians, journalists, and critics call for new models of feminism relevant to women today. As one of these viable models, popfeminism draws on dominant trends in mass culture, on pop’s forty-year history as a cultural prefix in Germany, and on traditional feminism in order to create a new, ostensibly apolitical, feminist subculture based in self-stylization and individual autonomy. Shared by many popfeminist sources is the depiction of negatively coded female corporeality. This article begins with a theoretical analysis of writings on sexuality and the body in recent (popfeminist nonfiction. It then examines the negative corporeal self-stylizations in Turkish-German rapper Lady Bitch Ray’s performances since 2006, in former music video host Charlotte Roche’s novel Feuchtgebiete (2008, and in media personality Sarah Kuttner’s novel Mängelexemplar (2009. Ultimately, these negatively coded bodies are shown to uncover popfeminism’s political intent.

  5. About the identification of behaviour law parameters of clayey rocks; Sur l'identification des parametres des lois de comportement des roches argileuses

    Energy Technology Data Exchange (ETDEWEB)

    Lecampion, B


    This work aims at developing identification methods for clayey rock parameters. These methods are necessary for the interpretation of the numerous data obtained at the ANDRA's Meuse/Haute-Marne underground laboratory. Two main rheological aspects have been considered: the poro-elastic behaviour and the elasto-visco-plastic behaviour. The first part of the study focusses on the poro-elastic parameters. Chapter 2 recalls the direct problem and discusses some important points of the identification inverse problem. Chapter 3 deals with the formulation of gradient calculation techniques for the linear poro-elastic case. The resolution using the finite-element method is discussed. The direct and associated state differentiation methods are validated for a 2D numerical example using the finite-element code Cast3M. The identification of poro-elastic coefficients of the Meuse/Haute-Marne argillaceous rocks is discussed in detail in chapter 4. The use of approximate semi-explicit solutions of the direct problems allows to obtain a fast identification method. The second part deals with the identification of elasto-visco-plastic parameters. The visco-plastic behaviour of Meuse/Haute-Marne rocks is discussed in chapter 5 and a visco-plastic model with nonlinear isotropic cold-drawing is proposed which allows to reproduce the tests. The parameters of this behaviour law are identified on a 1D creep test in drained conditions. Thus, the delayed deformations come from the poro-elastic and visco-plastic behaviour of the rock. It is shown that both phenomena can be separated. All poro-elasto-visco-plastic parameters are identified and a semi-explicit solution of the creep test is used. Chapter 6 presents an identification method of the elasto-visco-plastic parameters for the general case. The identification is equivalent to the minimization of a cost functional. The gradient of the functional is calculated by direct differentiation. The direct differentiation method is developed in Cast3M for the Norton-Hoff elasto-visco-plastic law. The identification of the parameters of this law on 1D and 2D configurations of underground facilities is then presented. Measurements are simulated using a direct calculation, eventually disturbed. The unicity and stability of this identification problem are discussed. In chapter 7, the method is applied to a real case: the Chamoise tunnel. 1D and 2D flat deformation modeling is used to solve the direct problem. The modeling hypotheses are justified and the obtained parameters allow to correctly reproduce the data. (J.S.)

  6. Analytical and clinical comparison of Elecsys syphilis (Roche®) - Architect syphilis TP and reformulated Architect syphilis TP (Abbott®) assay. (United States)

    De Keukeleire, Steven; Desmet, Stefanie; Lagrou, Katrien; Oosterlynck, Julie; Verhulst, Manon; Van Besien, Jessica; Saegeman, Veroniek; Reynders, Marijke


    The performance of Elecsys Syphilis was compared to Architect Syphilis TP and Reformulated Architect Syphilis TP. The overall sensitivity and specificity were 98.4% and 99.5%, 97.7% and 97.1%, and 99.2% and 99.7% respectively. The assays are comparable and considered adequate for syphilis screening. Copyright © 2016 Elsevier Inc. All rights reserved.

  7. Fractures inside crystalline rocks. Effects of deformations on fluid circulations; Fractures dans les roches cristallines. Effets des deformations sur les circulations de fluides

    Energy Technology Data Exchange (ETDEWEB)

    Gentier, S


    The modeling of fluid flows inside granite massifs is an important task for the evaluation of the feasibility of radioactive waste storage inside such formations. This document makes a synthesis of the works carried out since about 15 years, in particular by the French bureau of geological and mining research (BRGM), about the hydro-mechanical behaviour of a fracture and about the hydrodynamical characterization of fracture networks inside crystalline rocks: 1 - introduction; 2 - hydro-mechanical behaviour under normal stress: experimental results (hydro-mechanical behaviour, flow regimes, mechanical behaviour, test protocol, complementary tests, influence of samples size), geometrical interpretation of experimental results (relation with walls geometry, relation with voids geometry, relation with contacts geometry), hydro-mechanical modeling (hydraulic modeling, mechanical modeling); 3 - from the hydro-mechanical behaviour under normal stress to the coupling with heat transfers and chemistry: experiment for the study of the chemo-thermo-hydro-mechanical coupling (experimental results, relation with walls morphology), thermo-hydro-mechanical experiments, thermo-hydro-chemical experiments with fractures, conclusions; 4 - hydro-mechanical behaviour during shear: experimental results, geometrical interpretation (relation with the geometry of damaged zones, relation with voids geometry, relation with walls geometry), hydro-mechanical modeling (mechanical modeling, hydro-mechanical modeling of the behaviour during shear). (J.S.)

  8. Etude expérimentale de la diffusion thermique dans les monocristaux d'olivine et dans les roches du manteau supérieur


    GIBERT, Benoit


    The knowledge of the thermal structure of the Earth is a prerequisite to better understand the geological processes observed at the Earth's surface. Conductive and convective heat transport mechanisms within the mantle determine this thermal structure and are mainly controlled by heat transport properties of mantle rocks. This study proposes to investigate these properties by measuring the thermal diffusivity of olivine single-crystals and polycristalline aggregates (rocks) at high temperatur...

  9. Propriétés mécaniques des roches argileuses carbonatées: Importance de la relation calcite-argile (United States)

    Bauer-Plaindoux, Corinne; Tessier, Daniel; Ghoreychi, Mehdi


    Mechanical properties and swelling of a clayey rock do not depend only on the quantity and the nature of the clay. Different experiments showed that the properties are a function of the material structure and texture. Clay properties can be more or less expressed and means that a carbonated phase may prevent clay hydration and swelling.

  10. Characterization of Rock Wettability Though Dielectric Measurements Caractérisation de la mouillabilité des roches au moyen de mesures diélectriques

    Directory of Open Access Journals (Sweden)

    Bona N.


    Full Text Available The wettability of glass filters and Berea sandstone was investigated using the electric response in the interval 10² - 10 (to the power of 8 Hz. The natural wettability of the materials was modified to get two different sets of samples, one with strong water and the other with strong oil wettability. The samples were saturated to various degrees up to 40% with deionized water or brine. Measurements showed that the electric responses of water-wet and oil-wet samples were markedly different and more complex than those predicted by two standard models. The dispersivity and the loss tangent were found to be the most suitable parameters to check the wettability of the samples. La mouillabilité de filtres de verre et de grès de Béréa a été caractérisée par leur réponse électrique dans l'intervalle 10² - 10 puissance 8 Hz. Au moyen de traitements appropriés, la mouillabilité naturelle des matériaux a été modifiée afin d'obtenir deux séries différentes d'échantillons ayant respectivement de fortes mouillabilités à l'eau et à l'huile. Les échantillons ont été saturés à des degrés variés (pas plus de 40 % avec de l'eau permutée ou de la saumure. Les mesures ont montré que les réponses électriques des échantillons mouillables à l'eau ou mouillables à l'huile étaient nettement différentes et plus compliquées que celles prédites par deux modèles standard. En outre, on a pu constater que la dispersivité et la tangente de pertes constituent les paramètres les plus pertinents pour caractériser la mouillabilité des échantillons.

  11. Visualisation des propriétés capillaires des roches réservoir Visualizing the Capillary Properties of Reservoir Rocks

    Directory of Open Access Journals (Sweden)

    Zinszner B.


    Full Text Available Cet article décrit des expériences de drainage par centrifugation et d'imbibition par ascension capillaire réalisées avec des résines époxy colorées. Après polymérisation, l'observation des lames minces permet de localiser les fluides mouillants et non mouillants. Après avoir décrit les modes opératoires en insistant sur l'analyse des paramètres expérimentaux, on donne des exemples d'applications à la géologie de réservoir. Deux points sont développés : les études de perméabilités et les modèles de réservoir qui permettent d'étudier la répartition du fluide mouillant et des fractions déplaçables ou piégées du fluide non mouillant. This article describes drainage experiments by centrifuge method and imbibition by capillary rise performed with colored epoxy resins. After polymerization, analysis of thin sections serves to situate the wetting and nonwetting fluids. After describing the operating methods with em-phasis on the analysis of experimental parameters, the article gives examples of applications to reservoir geology. The following two points are developed : (i permeability investigations and (ii reservoir modelswhich can be used to analyze the distribution of the wetting fluid and the movable or trapped fractions of the nonwetting fluid

  12. Cicatrization of the damaged zone around a tunnel in low permeable rocks; Cicatrisation de la zone endommagee autour du tunnel dans des roches peu permeables

    Energy Technology Data Exchange (ETDEWEB)

    Hamza, R.O


    Three types of clays have been studied in this work: the Mol clay (plastic clay), the Mont Terri clay (opalinus clay) and the East clay (stiff clay). This work is divided into three parts: an experimental part which has allowed to identify the cicatrization phenomenon and the cracks closing. Through several tests has been determined the evolution of the permeability of the clayey samples which are undamaged or damaged with time and according to the different applied hydro-mechanical loadings. In order to describe the cicatrization phenomenon, a rheological law has been developed from the experimental observations and under some hypotheses. The parameters of the cicatrization model have been determined by numerical simulation. In order to take into account the structures effects at a large scale, the modelling of a tunnel has allowed to better identify the cicatrization phenomenon and to analyze the long term stability of this work. (O.M.)

  13. Comparison of the digene hybrid capture 2 and Roche cobas 4800 HPV tests for detection of CIN2+ in a referral population in Japan. (United States)

    Sasagawa, Toshiyuki; Maehama, Toshiyuki; Osaka, Yasuhiro; Sakamoto, Jinichi; Shibata, Takeo; Fujita, Satoko; Takakura, Masahiro; Takagi, Hiroaki


    To examine validity of the hybrid capture-2 and cobas 4800 HPV tests, 396 women including 188 women visiting for cancer screening, and 208 referral cases were examined with both HPV tests and the liquid-based cervical Pap test. Concordant results between the HPV assays were observed in 333 cases (coincident rates; 84.1%, kappa value; 0.682). The sensitivity for CIN2+ was 98.6% (69/70) and 82.9% (58/70) for HC2 and cobas 4800 (McNemar's test; P = 0.0026). The sensitivity for CIN3+ was 97.2% (35/36) and 83.3% (30/36) (Not significant, P = 0.0736). The specificities for CIN2+ or CIN3+ did not differ between the tests. The HPV16, 52, 18, 31, and 58 were the most common types in CIN2+ cases. Reasonable sensitivity for HPV52, and cross-hybridization with some probable high-risk HPV type such as HPV82 explain the higher sensitivity of HC2 than cobas 4800 in detection of CIN2+ in a referral population in Japan. © 2018 Wiley Periodicals, Inc.

  14. Stratigraphic and technological evidence from the Middle Palaeolithic-Châtelperronian-Aurignacian record at the Bordes-Fitte rockshelter (Roches d'Abilly site, Central France). (United States)

    Aubry, Thierry; Dimuccio, Luca Antonio; Almeida, Miguel; Buylaert, Jan-Pieter; Fontana, Laure; Higham, Thomas; Liard, Morgane; Murray, Andrew S; Neves, Maria João; Peyrouse, Jean-Baptiste; Walter, Bertrand


    This paper presents a geoarchaeological study of Middle and Upper Palaeolithic (Châtelperronian, Aurignacian and Solutrean) occupations preserved at the Bordes-Fitte rockshelter in Central France. The lithostratigraphic sequence is composed of near-surface sedimentary facies with vertical and lateral variations, in a context dominated by run-off and gravitational sedimentary processes. Field description and micromorphological analysis permit us to reconstruct several episodes of sediment slope-wash and endokarst dynamics, with hiatuses and erosional phases. The archaeostratigraphic succession includes Châtelperronian artefacts, inter-stratified between Middle Palaeolithic and Aurignacian occupations. Systematic refitting and spatial analysis reveal that the Châtelperronian point production and flake blanks retouched into denticulates, all recovered in the same stratigraphic unit, result from distinct and successive occupations and are not a 'transitional' Middle to Upper Palaeolithic assemblage. The ages obtained by (14)C place the Châtelperronian occupation in the 41-48 ka cal BP (calibrated thousands of years before present) interval and are consistent with the quartz optically stimulated luminescence age of 39 ± 2 ka and feldspar infra-red stimulated luminescence age of 45 ± 2 ka of the sediments. The Bordes-Fitte rockshelter sequence represents an important contribution to the debate about the characterization and timing of the Châtelperronian, as well as its affinities to earlier and later industries. Copyright © 2011 Elsevier Ltd. All rights reserved.

  15. Natural fracturing of rocks: application to the Ahnet basin (Algeria); Fracturation naturelle des roches: application au bassin de l`Ahnet (Algerie)

    Energy Technology Data Exchange (ETDEWEB)

    Badsi, M.


    In the Ahnet basin, the production seems to be unrelated to lithological variations in the reservoirs. In these large anticline structures, located in the central Ahnet basin, the presence of gas has been proven, but only a few production wells have been moderately successful. This inconsistency is probably related to the spatial distribution of fracturing throughout the reservoir. In order to investigate several hypothesis, we used several approach to solve problems posed by the interpreter: namely understanding the deformation process, predicting the fractured zones and building the discrete model of fracture network. This approach combines several methods, including sand box modelling, numerical modelling and Statistics rules, often related with fractal behaviour of faults families, have been used for extrapolating observations from seismic or from wells. The numerical tools and sand box analysis have allowed us to answer to the questions related to the formation of this large anticlines in the Ahnet basin and suggest a probable origin of the variation in the spatial distribution of natural fractures. The deterministic predictions of small-scale faults use probabilistic approaches for spatial interpolation assuming implicitly relationship between detected large faults and unresolved small faults. The statistical modelling is used to carry out analysis of the spatial variation of mean fracture attributes at the global scale (a few kilometers) and a 3D stochastic modelling of the fracture system at the local scale (a few ten of meters). (author) 139 refs.

  16. Personalised medicine in interstitial lung diseases: Number 6 in the Series "Personalised medicine in respiratory diseases" Edited by Renaud Louis and Nicolas Roche. (United States)

    Kokosi, Maria A; Margaritopoulos, George A; Wells, Athol U


    Interstitial lung diseases in general, and idiopathic pulmonary fibrosis in particular, are complex disorders with multiple pathogenetic pathways, various disease behaviour profiles and different responses to treatment, all facets that make personalised medicine a highly attractive concept. Personalised medicine is aimed at describing distinct disease subsets taking into account individual lifestyle, environmental exposures, genetic profiles and molecular pathways. The cornerstone of personalised medicine is the identification of biomarkers that can be used to inform diagnosis, prognosis and treatment stratification. At present, no data exist validating a personalised approach in individual diseases. However, the importance of the goal amply justifies the characterisation of genotype and pathway signatures with a view to refining prognostic evaluation and trial design, with the ultimate aim of selecting treatments according to profiles in individual patients. Copyright ©ERS 2018.

  17. Prevalence of human papillomavirus in 5,072 consecutive cervical SurePath samples evaluated with the Roche cobas HPV real-time PCR assay

    DEFF Research Database (Denmark)

    Preisler, Sarah; Rebolj, Matejka; Untermann, Anette


    New commercially available Human Papillomavirus (HPV) assays need to be evaluated in a variety of cervical screening settings. Cobas HPV Test (cobas) is a real-time PCR-based assay allowing for separate detection of HPV genotypes 16 and 18 and a bulk of 12 other high-risk genotypes. The aim...... years, an extra 11% of women would based on historical data be expected to have a positive cobas test without an underlying cervical intraepithelial lesion grade 3 or worse. Countries with a high prevalence of HPV infections should therefore proceed to primary HPV-based cervical screening with caution....... of the present study, Horizon, was to assess the prevalence of high-risk HPV infections in an area with a high background risk of cervical cancer, where women aged 23-65 years are targeted for cervical screening. We collected 6,258 consecutive cervical samples from the largest cervical screening laboratory...

  18. Performance Evaluation of Manual and Automated (MagNA Pure Nucleic Acid Isolation in HPV Detection and Genotyping Using Roche Linear Array HPV Test

    Directory of Open Access Journals (Sweden)

    Aikaterini Chranioti


    Full Text Available Nucleic acids of human papillomavirus (HPV isolated by manual extraction method (AmpliLute and automated MagNA pure system were compared and evaluated with cytohistological findings in 253 women. The concordance level between AmpliLute and MagNA was very good 93.3% (=0.864, <.0001. Overall HPVpositivity detected by AmpliLute was 57.3% (30.4% as single and 27% as multiple infections in contrast to MagNA 54.5% (32% and 23%, resp.. Discrepant results observed in 25 cases: 11 MagNA(−/AmpliLute(+, 10 of which had positive histology; 5 MagNA(+/AmpliLute(− with negative histology; 8 MagNA(+/AmpliLute(+: in 7 of which AmpliLute detected extra HPV genotypes and 1 MagNA(invalid/AmpliLute(+ with positive histology. Both methods performed well when compared against cytological (area under curve (AUC of AmpliLute 0.712 versus 0.672 of MagNA and histological diagnoses (AUC of AmpliLute 0.935 versus 0.877 of MagNA, with AmpliLute showing a slightly predominance over MagNA. However, higher sensitivities, specificities, and positive/negative predictive values were obtained by AmpliLute.

  19. Damage-induced permeability changes around underground excavations; Endommagement des roches argileuses et permeabilite induite au voisinage d'ouvrages souterrains

    Energy Technology Data Exchange (ETDEWEB)

    Coll, C


    The storage of nuclear waste in deep geological formations is now considered more and more as a potential solution. During excavation, a disturbed zone develops in which damaging can be important and which can lead eventually to the failure of the rock. Fluid flow and permeability in the rock mass can be significantly modified producing a possible security risk. Our work consisted in an experimental study of the hydro-mechanical coupling of two argillaceous rocks: Boom clay (Mol, Belgium) and Opalinus clay (Mont-Terri, Switzerland). Triaxial tests were performed in a saturated state to study the permeability evolution of both clays with isotropic and deviatoric stresses. Argillaceous rocks are geo-materials with complex behaviour governed by numerous coupled processes. Strong physico-chemical interactions between the fluid and the solid particles and their very low permeability required the modification of the experimental set up. Moreover, specific procedures were developed to measure permeability and to detect strain localisation in shear bands. We show that for Boom Clay, permeability is not significantly influenced by strain localisation. For Opalinus clay, fracturing can induce an increase of the permeability at low confining pressure. (author)

  20. Creep and damage in argillaceous rocks: microstructural change and phenomenological modeling; Fluage et endommagement des roches argileuses: evolution de la microstructure et modelisation phenomenologique

    Energy Technology Data Exchange (ETDEWEB)

    Fabre, G


    The underground radioactive waste disposal far exceeds the period of exploitation of common civil engineering works. These specific projects require to predict the irreversible deformations over a large time scale (several centuries) in order to assess the extension and to forecast the evolution of the EDZ (Excavation Damage Zone) around the cavity. In this study, the viscosity of three sedimentary argillaceous rocks has been studied under different conditions of uniaxial compression: static or cyclic creep tests, monotonic and quasistatic tests, performed across various strata orientations. Argillaceous rocks are studied as a possible host layer for radioactive waste disposals. Indeed, they present some of the physical characteristics and mechanical properties, which are essential for being a natural barrier: low permeability, high creep potential and important holding capacity of radioactive elements. The purpose of the experimental study was to shed some light over the mechanisms governing the development of delayed deformations and damage of argillaceous rocks. It relates three rocks: an argillite from East of France, a Tournemire argillite and a marl from Jurassic Mountains. On atomic scale, viscoplastic deformations are due to irreversible displacements of crystalline defects, called dislocations. The experimental study was also supplemented with observations on thin sections extracted from the argillite and marl samples using a SEM. The aim was to identify the mechanisms responsible for the time-dependent behaviour on a microstructural scale. Analytical simulations of the mechanical behaviour of the three rocks gave parameters used in different viscoplastic models. The best modeling was obtained with the viscoplastic model which takes account of the development of volumetric strains and of the damage anisotropy. (author)

  1. Influence aqueous solutions on the mechanical behavior of argillaceous rocks; Influence des solutions aqueuses sur le comportement mecanique des roches argileuses

    Energy Technology Data Exchange (ETDEWEB)

    Wakim, J


    The hydration of the shale with an aqueous solution induces a swelling deformation which plays an important role in the behaviour of the structures excavated in this type of grounds. This deformation is marked by a three-dimensional and anisotropic character and involves several mechanisms like adsorption, osmosis or capillarity. Several researches were dedicated to swelling and were often much debated due to the complexity of the implied phenomena. The goal of this thesis is therefore to contribute to a better understanding of shale swelling when the rock is confined and hydrated with an aqueous solution. The main part of the work accomplished was related to the Lorraine shale and to the Tournemire shale. To characterize swelling and to identify the main governing parameters, it was necessary to start the issue with an experimental approach. Many apparatus were then developed to carry out tests under various conditions of swelling. In order to facilitate the interpretation of the tests and thereafter the modelling of the behaviour, the experimental procedure adopted consisted of studying first the mechanical aspect and then the chemical aspect of swelling. In the mechanical part, swelling was studied by imposing on the sample a mechanical loading while maintaining during the tests the same aqueous solution. The principal parameters which were studied are the effect of the lateral conditions on axial swelling (impeded strain or constant stress) as well as the influence of the axial stress on radial swelling. The anisotropy of swelling was studied by carrying out, for different orientations of the sample, tests of free swelling, impeded swelling and uniaxial swelling. These various mechanical tests allowed to study the three-dimensional anisotropic swelling in all the conditions and to select the most appropriate test to be used in the second phase of the research. The precise analysis performed to explain the mechanisms behind the swelling of an argillaceous rock when it is in contact of water showed that the mechanical response is ruled mainly by electrochemical phenomena which operate between the solution elements and the clay layers. This important result oriented the research to develop better the role of the chemistry in the behaviour. Swelling was therefore analysed by imposing on the sample a chemical loading while maintaining constant the axial stress. Monovalent and divalent salts which were used with various concentrations are the sodium chloride (NaCl), the potassium chloride (KCl) and the calcium chloride (CaCl{sub 2}). The foreseen objective was to analyze the effect of the chemical path with decreasing or cyclic concentrations and to highlight the role of the solution normality, the type of salt and the anisotropy on the axial swelling. The experimental results obtained allowed the development of a new rheological model which takes into account the time, the anisotropy, the applied loading and the chemistry of the solution. This model was implemented in a finite element code to simulate simple cases and to prove its validity. (author)

  2. Reduction of Polymer Adsorption on Reservoir Rocks Réduction de l'adsorption des polymères sur les roches réservoirs

    Directory of Open Access Journals (Sweden)

    Chauveteau G.


    Full Text Available The adsorption properties of polyacrylamides and xanthans on mineral surfaces carrying silanol and aluminol groups such as sand and kaolinite are described. The influence of the main parameters such as the nature of adsorption sites, surface charge, chemical structure and conformation of polymer and interactions of mono- and divalent ions with polymer and mineral surface has been investigated and interpreted. Some operating parameters in polymer flooding such as pH and salinity of injected solution, the nature of the polymer and its degree of ionicity were found to be determining factors from the adsorption level. The results give key elements for reducing adsorption by a proper choice of polymer nature and ionicity and of injection conditions. Les propriétés d'adsorption des polyacrylamides et des xanthanes sur des surfaces minérales portant des groupements silanols et aluminols comme le sable et la kaolinite ont été examinées. L'influence de différents paramètres est analysée : nature des sites d'adsorption, charge de surface, structure chimique et conformation du polymère, interactions des ions mono et divalents avec la surface. Il apparaît que le pH et la salinité de la solution injectée, la nature du polymère et, en particulier son degré d'ionicité, qui sont les paramètres opérationnels lors d'une injection de polymère destinée à augmenter le taux de récupération du pétrole, sont déterminants en ce qui concerne les niveaux d'adsorption. On en déduit les principaux moyens pour réduire l'adsorption dans un cas d'application donné.

  3. Stratigraphic and technological evidence from the middle palaeolithic-Châtelperronian-Aurignacian record at the Bordes-Fitte rockshelter (Roches d’Abilly site, Central France)

    DEFF Research Database (Denmark)

    Aubry, Thierry; Dimuccio, Luca Antonio; Almeida, Miguel


    includes Châtelperronian artefacts, inter-stratified between Middle Palaeolithic and Aurignacian occupations. Systematic refitting and spatial analysis reveal that the Châtelperronian point production and flake blanks retouched into denticulates, all recovered in the same stratigraphic unit, result from...

  4. “Knaller-Sex für alle”: Popfeminist Body Politics in Lady Bitch Ray, Charlotte Roche, and Sarah Kuttner


    Carrie Smith-Prei


    Germany has seen a recent upsurge in publications proclaiming that feminism is again an urgent matter for a new generation of women. Faced with the reactionary demography debate and the hegemony of second-wave feminism, young writers, musicians, journalists, and critics call for new models of feminism relevant to women today. As one of these viable models, popfeminism draws on dominant trends in mass culture, on pop’s forty-year history as a cultural prefix in Germany, and on traditional femi...

  5. Approach to underground characterization of a disposal vault in granite; Methode de caracterisation souterraine d`une enceinte de stockage dans la roche granitique

    Energy Technology Data Exchange (ETDEWEB)

    Everitt, R.A.; Martin, C.D.; Thompson, P.M.


    The concept of disposing of nuclear fuel waste by sealing it in a disposal vault in the Canadian Shield is being investigated as part of the Canadian Nuclear Fuel Waste Management Program. Engineered and natural barriers would isolate the waste from the biosphere. Underground characterization and testing have been under way since 1983 at the Underground Research Laboratory in support of this program. This report draws on experience gained at the URL to recommend an approach to underground characterization to obtain information to optimize the design of the excavation and the engineered barriers, and to provide a baseline against which to monitor the performance of the facility during and following its operation.

  6. Benefit of Hepatitis C Virus Core Antigen Assay in Prediction of Therapeutic Response to Interferon and Ribavirin Combination Therapy


    Takahashi, Masahiko; Saito, Hidetsugu; Higashimoto, Makiko; Atsukawa, Kazuhiro; Ishii, Hiromasa


    A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor test, version 2 (Amplicor M), to estimate the predictive benefit of a sustained viral response (SVR) and non-SVR in 44 genotype 1b patients treated with interferon (IFN) ...

  7. Hepatitis B virus activity in patients with anti-hepatitis C virus antibody positivity and hepatitis B antigen positivity. (United States)

    Haushofer, Alexander C; Hauer, René; Brunner, Harald; Köller, Ursula; Trubert-Exinger, Doris; Halbmayer, Walter Michael; Haas, Josef; Kessler, Harald H


    Co-infection with hepatitis B virus (HBV) and HCV seems to be relatively frequent. There might be a mutual influence on replication activity of HBV and HCV. To determine the HBV activity in patients with serum HCV RNA and HBsAg positivity and in those with confirmed anti-HCV antibody and HBsAg positivity but serum HCV RNA negativity. A total of 1,200 anti-HCV antibody positive samples were investigated. Samples of HCV RNA and HBsAg positive patients were compared with those of confirmed anti-HCV and HBsAg positive but serum HCV RNA negative patients. HBV activity was tested with the quantitative Cobas Amplicor HBV Monitor Test (Roche Diagnostic Systems, Pleasanton, CA). Of all studied patients with chronic hepatitis C (serum HCV RNA positivity) only 1.0% were found to be HBsAg positive. In contrast, of all patients with confirmed anti-HCV positivity but serum HCV RNA negativity, 11.9% tested HBsAg positive. The median of HBV DNA levels of patients with serum HCV RNA positivity and HBeAg seroconversion (4.0 x 10(2) HBV DNA copies per ml) was found to be slightly lower than that of patients with serum HCV RNA negativity and HBeAg seroconversion (2.5 x 10(3) HBV DNA copies per ml; P>0.05). The median of HBV DNA levels of patients with serum HCV RNA positivity but without HBeAg seroconversion (1.1 x 10(4) HBV DNA copies per ml) was found to be significantly lower than that of patients with serum HCV RNA negativity but without HBeAg seroconversion (2.6 x 10(7) HBV DNA copies per ml; P<0.05). A mutual effect on HBV and HCV replication could be observed. The molecular assay for quantification of serum HBV DNA was found to be useful for the routine diagnostic laboratory.

  8. Survival of infants born to HIV-positive mothers, by feeding modality, in Rakai, Uganda.

    Directory of Open Access Journals (Sweden)

    Joseph Kagaayi

    Full Text Available BACKGROUND: Data comparing survival of formula-fed to breast-fed infants in programmatic settings are limited. We compared mortality and HIV-free of breast and formula-fed infants born to HIV-positive mothers in a program in rural, Rakai District Uganda. METHODOLOGY/PRINCIPAL FINDINGS: One hundred eighty two infants born to HIV-positive mothers were followed at one, six and twelve months postpartum. Mothers were given infant-feeding counseling and allowed to make informed choices as to whether to formula-feed or breast-feed. Eligible mothers and infants received antiretroviral therapy (ART if indicated. Mothers and their newborns received prophylaxis for prevention of mother-to-child HIV transmission (pMTCT if they were not receiving ART. Infant HIV infection was detected by PCR (Roche Amplicor 1.5 during the follow-up visits. Kaplan Meier time-to-event methods were used to compare mortality and HIV-free survival. The adjusted hazard ratio (Adjusted HR of infant HIV-free survival was estimated by Cox regression. Seventy-five infants (41% were formula-fed while 107 (59% were breast-fed. Exclusive breast-feeding was practiced by only 25% of breast-feeding women at one month postpartum. The cumulative 12-month probability of infant mortality was 18% (95% CI = 11%-29% among the formula-fed compared to 3% (95% CI = 1%-9% among the breast-fed infants (unadjusted hazard ratio (HR = 6.1(95% CI = 1.7-21.4, P-value < 0.01. There were no statistically significant differentials in HIV-free survival by feeding choice (86% in the formula-fed compared to 96% in breast-fed group (Adjusted RH = 2.8[95%CI = 0.67-11.7, P-value = 0.16] CONCLUSIONS/SIGNIFICANCE: Formula-feeding was associated with a higher risk of infant mortality than breastfeeding in this rural population. Our findings suggest that formula-feeding should be discouraged in similar African settings.

  9. Real-time PCR assays for hepatitis C virus RNA (genotype 1) is useful for evaluating virological response to the treatment with peginterferon-alpha2b and ribavirin. (United States)

    Nakaya, Mika; Enomoto, Masaru; Fujii, Hideki; Kobayashi, Sawako; Iwai, Shuji; Morikawa, Hiroyasu; Tamori, Akihiro; Sakaguchi, Hiroki; Kawada, Norifumi


    The real-time PCR, such as Abbott RealTime assay, have replaced end-point PCR, such as Amplicor assays, for the measurement of HCV RNA. However, 'response-guided therapy' to use on-treatment response for tailoring the duration of treatment with peginterferon-alpha and ribavirin has not been fully evaluated for real-time PCR. 43 patients with HCV genotype 1 (24 who had complete early virological responses (cEVR) on Amplicor assay and received 48-week therapy, and 19 who had late virological responses (LVR) and received 72-week therapy) were recruited. Using a RealTime assay, we retrospectively measured HCV RNA in stored sera. In 10 samples obtained during therapy, HCV RNA was undetectable on the Amplicor assay, but detectable on the RealTime assay. Among patients with cEVR on the Amplicor assay, those with detectable HCV RNA on the RealTime assay at week 12 were less likely to have a sustained virological response (SVR) than those without (2/4 vs 17/20, p = 0.116). Among patients with LVR on the Amplicor assay, those with HCV RNA detectable on the RealTime assay at week 24 were significantly less likely to have SVR than those without (1/4 vs 12/15, p = 0.041). The RealTime assay may be useful for tailoring duration of treatment for the patient with HCV genotype 1.

  10. Composite microstructural anisotropies in reservoir rocks: consequences on elastic properties and relation with deformation; Anisotropies microstructurales composites dans les roches reservoir: consequences sur les proprietes elastiques et relation a la deformation

    Energy Technology Data Exchange (ETDEWEB)

    Louis, L.


    From diagenesis to tectonic stress induced deformation, rock microstructures always present some anisotropy associated with a preferential orientation, shape or spatial arrangement of its constituents. Considering the consequences anisotropy has on directional transport properties and compliance, as the geological history it carries, this approach has received a particular attention in numerous works. In this work, the microstructural features of various sedimentary rocks were investigated through direct observations and laboratory measurements in naturally deformed and undeformed blocks, samples being considered as effective media. All investigated samples were found to be anisotropic with respect to the physical properties we measured (i.e. ultrasonic P-wave velocity, magnetic susceptibility, electrical conductivity). Considering that P-wave velocities can be described by a second order tensor, we applied to the velocity data the same inversion procedure as the one routinely used in magnetic studies, which provided an efficient tool to estimate and compare these 3D anisotropies with respect to the original sample geographical position. In each case, we tried to identify as thoroughly as possible the microstructural source of the observed anisotropies, first by the mean of existing models, then through direct observations (optic and electronic microscopy). Depending on the rock investigated, anisotropy was found to be controlled by pore shape, intergranular contact distribution, preferentially oriented microcracks interacting with compaction pattern or pressure solution cleavages interacting with each other. The net result of this work is that P-wave velocity anisotropy can express the interaction between different microstructural features as well as their evolution during deformation. (author)

  11. Perturbation des environnements marins à la limite Frasnien-Famennien (Dévonien terminal) : apport de la géochimie inorganique et du magnétisme des roches.


    Riquier, Laurent


    The Late Devonian times are a transitional period characterized by important climatic varia-tions and by several environmental perturbations recorded both in the continental domain (mountain uplift, development of vascular plants) and marine domain (sea-level fluctuations, biological crisis, burial of organic matter). For this study, we focus our attention to the deposition of worldwide organic matter-rich sedimentary levels: the Late Frasnian Kellwasser horizons, and their impact on carbon c...

  12. A European multicientre study on the comparison of HBV viral loads between VERIS HBV assay and Roche COBAS® TAQMAN® HBV test, Abbott RealTime HBV assay, Siemens VERSANT HBV assay, and Qiagen artus HBV RG kit. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Izopet, Jacques; Lombardi, Alessandra; Marcos, MaAngeles; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Hepatitis B viral load testing is essential to treatment and monitoring decisions in patients with chronic Hepatitis B. Beckman Coulter has developed the VERIS HBV Assay (Veris) for use on the fully automated DxN VERIS Molecular Diagnostics System. 1 OBJECTIVES: To evaluate the clinical performance of the Veris HBV Assay at multiple EU laboratories STUDY DESIGN: Method comparison was performed with a total of 344 plasma specimens from HBV infected patients tested with Veris and COBAS ® TaqMan ® HBV Test (Cobas), 207 specimens tested with Veris and RealTime HBV Assay (RealTime), 86 specimens tested with Veris and VERSANT ® HBV Assay (Versant), and 74 specimens tested with Veris and artus ® HBV RG PCR kit (artus). Bland-Altman analysis showed average bias of -0.46 log 10 IU/mL between Veris and Cobas, -0.46 log 10 IU/mL between Veris and RealTime, -0.36 log 10 IU/mL between Veris and Versant, and -0.12 log 10 IU/mL between Veris and artus. Bias was consistent across the assay range. Patient monitoring results using Veris demonstrated similar viral load trends over time to Cobas, RealTime, and artus. The VERIS HBV Assay demonstrated comparable clinical performance, with varying degrees of negative bias, compared to other currently marketed assays for HBV DNA monitoring. This negative bias should be taken into consideration if switching monitoring methods to Veris. Copyright © 2017 Elsevier B.V. All rights reserved.

  13. Interaction entre le fumier enrichi, le calcaire et les différentes sources de phosphore issues de la roche phosphatée de Matongo sur un ferralsol de la région du Bututsi (Burundi

    Directory of Open Access Journals (Sweden)

    Rwigema, JB.


    Full Text Available Interaction between enriched farmyard manure with fertilizers, lime and different phosphorus sources obtained from the phosphatic rock from Matongo on a ferralitic soil from the region of Bututsi (Burundi. This study on maize growing in pots shows that (i in combination with other inputs (farmyard manure and lime, the most soluble phosphorus sources (triple and simple superphosphate can be replaced by partially acidulated rock phosphate (50 % obtained by mixing simple superphosphate and rock phosphate both originating from the rock phosphate deposits in Matongo (Burundi, (ii the exchangeable Al can be reduced by both the addition of farmyard manure and lime, but the action of the different phosphorus 'source leave the most Bray-1 extractable phosphorus in the soil, the largest quantifies correspond to the highest doses of farmyard manure.

  14. Simulation of anions diffusion in Callovo-Oxfordian argillite by description of the rock microstructure; Modelisation de la diffusion des anions dans l'argilite du Callovo-oxfordien par description de la microstructure de la roche

    Energy Technology Data Exchange (ETDEWEB)

    Diaz, N. [CEA Saclay, Dept. de Physico-Chimie (DEN/DANS/DPC/SECR), 91 - Gif sur Yvette (France)


    The Callovo-Oxfordian argillite has been proposed as host rock for deep underground radioactive wastes storage. The aim of this study is to determine the report of effective diffusion coefficients De(anion)/De(water) at the micrometer scale considering the spatial distribution of minerals in the rock. (A.L.B.)

  15. An experimental study on mineral sequestration of CO{sub 2} in basics and ultra basics rocks; Etude experimentale des reactions de carbonatation minerale du CO{sub 2} dans les roches basiques et ultrabasiques

    Energy Technology Data Exchange (ETDEWEB)

    Dufaud, F


    The first part of the thesis is dedicated to dissolution data of siderite FeCO{sub 3} and magnetite Fe{sub 3}O{sub 4} which have been monitored in situ on the FAME beamline of the european synchrotron radiation facility in Grenoble. Iron in solution close to siderite single crystals is shown to be divalent hydrated. The small size of the experimentally investigated volume of solution (200 *400 micrometer and 3 mm height) allowed to work with single crystals in well defined geometries. No specific interaction was observed between iron (II) and dissolved inorganic carbon, suggesting that modelling siderite evolution under high CO{sub 2} pressures by using CO{sub 2}-less very acidic (pH 1-2) solutions is adequate. Using initial reaction rates, we get an activation energy for siderite dissolution of 62 kJ.mol{sup -1}, consistent with existing literature data. Such a value is suggestive of a mineral/solution interface mechanism.. Data from this study and from literature are consistent over a temperature range 25 C - 125 C and a pH range pH 1-7 with an empirical law: pk = pH + E{sub a}/(ln(10)*RT(K)) - log(S/V) - 10,5 where E{sub a} = 62 kJ.mol{sup -1} and S/V is the ratio between solid surface S and fluid volume V. A value of activation energy of 73.5 kJ.mol{sup -1} is obtained in the case of magnetite, also consistent with mineral/solution processes. The second and major part of the thesis work is the realization of analogical experiments for simulating carbonation of basic and ultra basic minerals. Experiments were carried out on consolidated rock cores at 90 C and 280 bar of CO{sub 2} (low temperature experiments) and on powders contained in metallic capsules at 400-500 C and 1000-1700 bars of CO{sub 2} (high temperature experiments). The rate of mineral storage of CO{sub 2} was defined as the molar ratio of solid carbonate formed over total CO{sub 2} injected. It is of about 1% in three months in low temperature experiments whereas it reaches several tens of percents per hour in high temperature experiments. In all cases, carbonation is shown to proceed according to dissolution/precipitation mechanisms. A quasi stoichiometric coupling is evidenced between carbonation and (proto) serpentinization in high-temperature experiments. Newly formed carbonates are mostly magnesite MgCO{sub 3} with Fe and Ca in solid solution. In low temperature samples, the silicates are covered with a thin silica layer and with carbonate spherules consisting of ankerite CaFe(CO{sub 3}){sub 2} - dolomite CaMg(CO{sub 3}){sub 2} - siderite cores surrounded by magnesite overgrowth. In CO{sub 2}-saturated water, peridotites are more reactive than serpentinite and basalts, in accordance with thermodynamic modelling whereas serpentinites are the most reactive in the supercritical CO{sub 2} phase, showing stronger reactivities in this latter phase than in CO{sub 2}-saturated water. In high temperature experiments, the rate of mineral storage is larger at 400 C than at 500 C, in agreement with thermodynamic modelling of the system. High water fugacities and high fluid salinities are shown to have a positive effect on mineral storage rates. Isotopic mass balance of carbon have evidenced that about 15% of the mineral storage consist of a reduced carbon phase, also identified by transmission electron microscopy as an ill-organized graphite phase. (author)

  16. Mechanical behaviour and rupture in clayey rocks studied by x-ray micro tomography; Comportement mecanique et rupture dans les roches argileuses etudies par micro tomographie a rayons X

    Energy Technology Data Exchange (ETDEWEB)

    Lenoir, N


    Within the framework of feasibility studies of underground repositories for radioactive waste, the study of permeability evolution with damage of the host layer is crucial. The goals of this work were: (i) to characterize experimentally the damage of two clayey rocks (BEAUCAIRE MARL and EAST SHALE) with x-ray micro tomography, (ii) to develop a high pressure triaxial set-up adapted to permeability measurement on very low permeability rocks.A number of original triaxial devices have been realised to characterize damage of clayey rocks, under deviatoric loading, with x-ray micro tomography on a synchrotron beamline at the ESRF (Grenoble). Localized damage and its evolution have been characterized at a fine scale (of order of ten microns). Digital image correlation techniques, extended to 3d images, have been used to measure incremental strain fields from tomographic images. we demonstrated that these techniques are very useful in the study of the localized damage of geo-materials and especially for the initiation. A high pressure triaxial device has been realised to measure permeability evolution of the east shale as a function of applied stress (isotropic and deviatoric). The particularity of this set-up is the small size of the test specimen (cylinder of 10 mm in diameter and 20 mm in height) which allows significant reduction of test duration. (author)

  17. Comparison of the Hologic Aptima HIV-1 Quant Dx Assay to the Roche COBAS Ampliprep/COBAS TaqMan HIV-1 Test v2.0 for the quantification of HIV-1 RNA in plasma samples

    DEFF Research Database (Denmark)

    Schønning, Kristian; Johansen, Kim; Landt, Bodil


    Background HIV-RNA is the most important parameter for monitoring antiviral treatment in individuals infected with HIV-1. Knowledge of the performance of different tests for the quantification of HIV-1 RNA is therefore important for clinical care. Objectives To compare the analytical performance ...

  18. Comparison of the Hologic Aptima HIV-1 Quant Dx Assay to the Roche COBAS Ampliprep/COBAS TaqMan HIV-1 Test v2.0 for the quantification of HIV-1 RNA in plasma samples. (United States)

    Schønning, Kristian; Johansen, Kim; Landt, Bodil; Benfield, Thomas; Westh, Henrik


    HIV-RNA is the most important parameter for monitoring antiviral treatment in individuals infected with HIV-1. Knowledge of the performance of different tests for the quantification of HIV-1 RNA is therefore important for clinical care. To compare the analytical performance of the Aptima HIV-1 Quant Dx Assay (Aptima) and the COBAS Ampliprep/COBAS TaqMan HIV-1 Test v2.0 (CAPCTMv2) for the quantification of HIV-1 RNA in plasma samples. The performance of the two tests was compared on 216 clinical plasma samples, on dilutions series in seven replicates of five clinical samples of known subtype and on ten replicates of the Acrometrix High and Low Positive Control. Bland-Altman analysis of 130 samples that quantified in both tests did not show indications of gross mis-quantification of either test. A tendency of the Aptima assay to quantify higher at high viral load compared to the CAPCTMv2 was observed in Bland-Altman analysis, by Deming regression (Slope 1.13) and in dilution series of clinical samples. Precision evaluated using the Acrometrix Positive Controls was similar for the High Control (CV: 1.2% vs. 1.3%; Aptima assay vs. CAPCTMv2 test, respectively), but differed for the Low control (CV: 17.9% vs. 7.1%; Aptima assay vs. CAPCTMv2 test, respectively). However, this did not impact clinical categorization of clinical samples at neither the 50 cp/mL nor 200 cp/mL level. The Aptima assay and the CAPCTMv2 test are highly correlated and are useful for monitoring HIV-infected individuals. Copyright © 2017 Elsevier B.V. All rights reserved.

  19. Processus métallogéniques associés aux interactions hydrothermales entre l'eau de mer et les roches ultrabasiques du manteau, exemple de la ride Médio-Atlantique


    Evrard, Catherine


    In this thesis , we studied hydrothermal sulfides that form in ultramafic environments along the Mid-Atlantic ridge , to clarify mantle hydrothermal processes and metallogenic processes behind enrichments metal sulphides . At the regional level , vulnerabilities low angle detachment , which bear the mantle rocks outcrop , control fluid circulation , while the engine of hydrothermal convection is activated locally by gabbroic intrusions within the mantle rocks . Hydrothermal sulfides studied i...

  20. Smallpox and pan-Orthodox Virus Detection by Real-Time 3’-Minor Groove Binder TaqMan Assays Oil the Roche LightCycler and the Cepheid Smart Cycler Platforms (United States)


    Bacillus anthracis BA0068 Ames Sterne SPS 97.13.213 Bacillus cereus Bacillus coagulans Bacillus licheniformis Bacillus macerans Bacillus ...megaterium Bacillus polymyxa Bacillus sphaericus Bacillus stearothermophilus Bacillus subtilis subsp. niger Bacillus thuringiensis Bacillus popilliae...varicella- zoster virus, and Bacillus anthracis DNA by LightCycler polymerase chain reaction after autoclaving:

  1. Geochemical modeling of water-gas-rock interactions. Application to mineral diagenesis in geologic reservoirs; Modelisation geochimique des interactions eau-gaz-roche. Application a la diagenese minerale dans les reservoirs geologiques

    Energy Technology Data Exchange (ETDEWEB)

    Bildstein, O.


    The Ph.D. report describes a conceptual and numerical model for simulating gas-water-rock interaction during mineral diagenesis of sediments. The main specific features of this model are the following: applicable to open systems, half-implicit resolution numerical method, feedback on the texture evolution (grain model), existence of a gas phase, oxido-reduction phenomena. (author) 217 refs.

  2. Post-Skinnerian, Post-Skinner, or Neo-Skinnerian? Hayes, Barnes-Holmes, and Roche's Relational Frame Theory: A Post-Skinnerian Account of Human Language and Cognition (United States)

    Ingvarsson, Einar T.; Morris, Edward K.


    The perceived inability of behaviorism to deal with complex human behavior has been a recurrent theme among its critics. Although ingenious and subtle, even Skinner's Verbal Behavior (1957) is widely faulted on these grounds, in particular, for failing to explain linguistic generativity (Chomsky, 1959). In Relational Frame Theory: A…

  3. POCT PT INR - Is it adequate for patient care? A comparison of the Roche Coaguchek XS vs. Stago Star vs. Siemens BCS in patients routinely seen in an anticoagulation clinic. (United States)

    Baker, Wendy S; Albright, Kathleen J; Berman, Megan; Spratt, Heidi; Mann, Peggy A; Unabia, Jaime; Petersen, John R


    In this study we examined the difference in patient INR values as measured by the POCT CoaguChek XS device and central laboratory Stago Evolution and Siemens BCS XP analyzers. This study composed of 100 warfarin therapy patients and 20 coagulation normal subjects, showed that the difference between the POCT and clinical laboratory values increased with increasing INR and was exacerbated by the use of different thromboplastin reagents by the POCT and central lab. The CoaguChek XS and on-site Stago analyzers which used human recombinant (ISI=1.01) and rabbit brain thromboplastin (ISI=1.25), respectively, showed reasonable agreement for INRSiemens BCS XP, which both employed human recombinant thromboplastin (BCS ISI=1.02), showed greater agreement for the complete range INR values (INR5.0 with the Stago Evolution prevented adverse treatment events for the warfarin therapy patients in the six months preceding and following this investigation. Copyright © 2017 Elsevier B.V. All rights reserved.

  4. The Cretaceous Source Rocks in the Zagros Foothills of Iran Les roches mères Crétacé de l'avant-pays du Zagros en Iran : un exemple de bassin intracratonique de grande dimension

    Directory of Open Access Journals (Sweden)

    Bordenave M. L.


    Full Text Available Almost all the oil production of Iran is concentrated in a small portion of the Zagros orogenic belt, the 40,000 Sq km Dezful Embayment of the Khuzestan province. The accumulation of oil results from a Cretaceous/Tertiary petroleum complex which includes 6 source rock layers of unequal importance, i. e. the basal part of the Garau Formation (Neocomian, the Gadvan (Barremian, the Kazhdumi (Albian, the Ahmadi (Early Cenomanian, the Gurpi (Senonian and the Pabdeh (Eocene. These layers generated 99% of the onshore Iranian oil reserves, trapped into two main reservoirs, the Asmari (Early Miocene and Bangestan (Cenomanian limestones which contained 330 billions barrels of original oil in place, corresponding to more than 7% of the current global reserves. This paper discusses the distribution of Cretaceous source rocks in the Iranian Foothills and the Iranian side of the Gulf, and the geological, oceanological and climatic factors which favored dysoxic to anoxic conditions and the deposition of organic-rich sediments. The impact of each of these source rocks on oil accumulations is also briefly considered. Among Cretaceous source rocks, two of them, the Neocomian Garau deposited over Lurestan, NE Khuzestan and the northeastern part of the Gulf, and the Albian Kazhdumi, deposited in the central part of the Dezful Embayment, have excellent characteristics with initial organic carbon sometimes higher than 10%, Hydrogen Indices (HI in the 600 g HC/kg C range and maximum thickness in excess of 300 m. Their organic matter is mainly algal with high sulfur content, qualifying them as IIs type. They were deposited in well established depressions included in an overall shallow water intracratonic basin communicating with the Southern Tethys Ocean. These depressions became anoxic as the result of a water stratification caused by the conjunction of high stand waters, humid climate and the existence of a sill separating the depression from the open sea. On the contrary, the Gadvan and Gurpi Formations were deposited in poorly defined depressions where dysoxic conditions prevailed in few limited areas only, i. e. SE Khuzestan and NW Fars for the Gadvan and in a narrow NW-SE trough extending from Lurestan to North Fars for the Gurpi. In these areas, Gadvan and Gurpi have only marginal potentials, TOC values in the 1-2% range and HI varying from 150 to 400 g HC/kg C. The impact of the various source rocks is extremely variable. In Lurestan, rich Garau potential source rocks are seldom associated with valid traps. The basinal Oligosteginatight facies of the Sarvak remains most often associated with Garau facies, and the excellent Asmari reservoir is breached in the Lurestan structures as the result of the Lurestan uplift during Pliocene. Therefore, only few small fields are related to Garau source rocks, accumulated in the Sarvalk and Ilam, when deposited under a high energy facies in SE Lurestan. In Central and Southern Dezful Embayment, very prolific thick mature Kazhdumi source rocks associated with excellent reservoirs, very large structures and the efficient Gachsaran cap rock, formed one of the most efficient Petroleum Systems, which would account for the accumulation of more than 95% of the onshore Iranian oil in place. An impressive gathering of giant fields, namely Ahwaz, Agha Jari, Bibi Hakimeh, Gachsaran, Mansuri, Marun and Rag-e Sefid, located in an area of 40 000 Sq km, are originated from the Kazhdumi source rocks. The contribution of other Cretaceous source rocks, i. e. Gadvan and Gurpi, is assumed to be negligible, because of their poor characteristics and their limited areal extent. The Gurpi is immature or marginally mature in most of the study area, with the exception of the NE Khuzestan and the deeper synclinorial areas of Central Khuzestan, where it may have generated very limited amounts of oil. La plus grande partie de la production pétrolière d'Iran est concentrée dans une petite partie de la chaîne du Zagros, constituée par les 40 000 km2 du Dezful Embayment , une zone déprimée située dans l

  5. A European multicientre study on the comparison of HIV-1 viral loads between VERIS HIV-1 Assay and Roche COBAS® TAQMAN® HIV-1 test, Abbott RealTime HIV-1 Assay, and Siemens VERSANT HIV-1 Assay. (United States)

    Braun, Patrick; Delgado, Rafael; Drago, Monica; Fanti, Diana; Fleury, Hervé; Hofmann, Jörg; Izopet, Jacques; Kühn, Sebastian; Lombardi, Alessandra; Mancon, Alessandro; Marcos, Mª Angeles; Mileto, Davide; Sauné, Karine; O'Shea, Siobhan; Pérez-Rivilla, Alfredo; Ramble, John; Trimoulet, Pascale; Vila, Jordi; Whittaker, Duncan; Artus, Alain; Rhodes, Daniel


    Viral load monitoring is essential for patients under treatment for HIV. Beckman Coulter has developed the VERIS HIV-1 Assay for use on the novel, automated DxN VERIS Molecular Diagnostics System. ¥ OBJECTIVES: Evaluation of the clinical performance of the new quantitative VERIS HIV-1 Assay at multiple EU laboratories. Method comparison with the VERIS HIV-1 Assay was performed with 415 specimens at 5 sites tested with COBAS ® AmpliPrep/COBAS ® TaqMan ® HIV-1 Test, v2.0, 169 specimens at 3 sites tested with RealTime HIV-1 Assay, and 202 specimens from 2 sites tested with VERSANT HIV-1 Assay. Patient monitoring sample results from 4 sites were also compared. Bland-Altman analysis showed the average bias between VERIS HIV-1 Assay and COBAS HIV-1 Test, RealTime HIV-1 Assay, and VERSANT HIV-1 Assay to be 0.28, 0.39, and 0.61 log 10 cp/mL, respectively. Bias at low end levels below 1000cp/mL showed predicted bias to be DxN VERIS System demonstrated comparable clinical performance to COBAS ® HIV-1 Test, RealTime HIV-1 Assay, and VERSANT HIV-1 Assay. Copyright © 2017 Elsevier B.V. All rights reserved.

  6. Feasibility study for the installation of a small hydro electric power plant; Etude de faisabilite. Petite centrale hydro-electrique au lieu dit 'Sous les Roches' a Sonceboz

    Energy Technology Data Exchange (ETDEWEB)

    Tissot, N. [MHyLab, Mini-Hydraulics Laboratory, Montcherand (Switzerland); Hausmann, H. [Hans Hausmann, Bevilard (Switzerland)


    This report for the Swiss Federal Office of Energy presents a technical, economical and ecological analysis of the feasibility of a small hydroelectric power plant at Sonceboz, in the Swiss Jura mountains. The power of the planed plant would typically be 500 kW for a water head of about 10 m. The study shows that, compared to one single turbine, a pair of Kaplan type turbines would be operational over a larger range of the river's water flow rate. This solution would be a little bit more costly but offer more flexibility for maintenance. Two maximum water flow rates are considered, 4.5 and 6 m{sup 3}/s respectively. According to the economical study, done with current electric kWh prices, both configurations are viable while the largest flow rate leads to a larger profit. The report is rounded up by a sensitivity analysis considering variations in annual power generation, electro-mechanical and construction cost as well as turbine efficiency. It indicates that this last factor could be crucial for the overall profitability. [French] Ce rapport est le resultat detaille de l'analyse technico-economique et ecologique de faisabilite de la realisation d'une petite centrale hydroelectrique basse chute qui pourrait exploiter une denivellation d'environ 10 m pour une puissance de l'ordre de 500 kW pres de Sonceboz dans le Jura suisse. L'etude montre que le couplage de deux turbines Kaplan offrirait une possibilite de fonctionnement sur une plus grande plage de flux ainsi qu'une plus grande flexibilite de maintenance, ceci pour un cout legerement superieur a l'option a une seule turbine. Les debits de 4.5 et 6 m{sup 3}/s sont consideres. L'etude economique montre que le debit de 6 m{sup 3}/s est plus rentable au prix de vente actuel du kWh. L'article se termine par une analyse de sensibilite sur les incertitudes concernant la production annuelle, les prix de l'electromecanique et du genie civil et le rendement des turbines.

  7. Optimization of a Laboratory-Developed Test Utilizing Roche Analyte-Specific Reagents for Detection of Staphylococcus aureus, Methicillin-Resistant S. aureus, and Vancomycin-Resistant Enterococcus Species▿


    Mehta, Maitry S.; Paule, Suzanne M.; Hacek, Donna M.; Thomson, Richard B.; Kaul, Karen L.; Peterson, Lance R.


    Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.

  8. NMR studies of the molecules dynamics to the solid-liquid interfaces: from graded porous materials to oil rocks; Etudes RMN de la dynamique des molecules aux interfaces solide-liquide: des materiaux poreux calibres aux roches petroliferes

    Energy Technology Data Exchange (ETDEWEB)

    Godefroy, S.


    Low field NMR relaxation for laboratory or in-situ applications provides critical information for oil recovery such as porosity, saturation, and permeability of rocks. In addition, pore size distribution and wettability can also be obtained in some cases. The technique relies on the measurement of proton longitudinal (T{sub 1}) or transverse (T{sub 2}) nuclear relaxation times. For better predictions, the surface micro-dynamics and the chemical properties of the liquids entrapped in the pore space are important and must be characterized. It is well known that the NMR relaxation is enhanced by the paramagnetic impurities at the pore surface but many other parameters influence the relaxation time distributions. These parameters are used to derive the petrophysical properties of the rocks. We propose here an original method to probe the dynamics of water and oil at the pore surface. In the present study, we used both nuclear relaxation at 2.2 MHz and field cycling Nuclear Magnetic Relaxation Dispersion (NMRD) techniques. We applied these two techniques to different kinds of water or oil saturated macroporous media (grain packings, outcrop and reservoir rocks with SiO{sub 2} or CaCO{sub 3} surfaces). We studied the dependence of NMR relaxation on pore size, magnetic field and temperature. Varying the pore size and the surface density of paramagnetic impurities of water saturated grain packings allowed experimental evidence for the two limiting regimes of the water relaxation in pores (surface- and diffusion-limited regimes). NMRD technique (evolution of 1/T{sub 1} with the magnetic field) allowed us to probe liquid surface dynamics in water or oil fully saturated grain packing, outcrop rocks or reservoir rocks (water- and oil-wet surfaces). We evidenced a two-dimensional molecular surface diffusion and directly estimated important parameters such as correlation times, residence times and molecular self-diffusion on the surface. Finally, we proved that the temperature dependence of T{sub 1} and T{sub 2} is directly related to the surface chemistry of the rocks. Such a dependence is clearly important for oil field in-situ measurements (well logging). (author)

  9. Development of a X-ray micro-tomograph and its application to reservoir rocks characterization; Developpement d`un microtomographe X et application a la caracterisation des roches reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira de Paiva, R.


    We describe the construction and application to studies in three dimensions of a laboratory micro-tomograph for the characterisation of heterogeneous solids at the scale of a few microns. The system is based on an electron microprobe and a two dimensional X-ray detector. The use of a low beam divergence for image acquisition allows use of simple and rapid reconstruction software whilst retaining reasonable acquisition times. Spatial resolutions of better than 3 microns in radiography and 10 microns in tomography are obtained. The applications of microtomography in the petroleum industry are illustrated by the study of fibre orientation in polymer composites, of the distribution of minerals and pore space in reservoir rocks, and of the interaction of salt water with a model porous medium. A correction for X-ray beam hardening is described and used to obtain improved discrimination of the phases present in the sample. In the case of a North Sea reservoir rock we show the possibility to distinguish quartz, feldspar and in certain zone kaolinite. The representativeness of the tomographic reconstruction is demonstrated by comparing the surface of the reconstructed specimen with corresponding images obtained in scanning electron microscopy. (author). 58 refs., 10 tabs., 71 photos.

  10. Detecção do DNA de Chlamydia trachomatis em espondiloartropatias e artrite reumatóide Detection of Chlamydia trachomatis DNA in spondyloarthropathies and rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Rafael Navarrete Fernandez


    Full Text Available Chlamydia trachomatis é a bactéria responsável pela doença sexualmente transmissível mais prevalente no mundo. A maioria das infecções em homens e mulheres é assintomática e, quando não diagnosticada e tratada, pode causar artrite e complicações relacionadas ao aparelho reprodutor feminino. OBJETIVO: pesquisar o DNA de C. trachomatis no líquido sinovial e urina de pacientes com espondiloartropatias e artrite reumatóide (AR, avaliar a presença de anticorpos séricos IgG e IgM anti-C. trachomatis nesses dois grupos de doenças e identificar o antígeno HLA-B27 em pacientes com espondiloartropatias. MÉTODOS: a população do estudo consistiu em 15 pacientes com espondiloartropatias: nove com espondiloartropatia indiferenciada (EI e seis com artrite reativa (ARe (grupo I e 15 pacientes com AR (grupo II. O DNA clamidial foi pesquisado em amostras de líquido sinovial e urina de todos os pacientes, empregando-se a PCR (Amplicor Roche, Suíça. Os anticorpos IgG e IgM anticlamidiais foram quantificados por imunofluorescência indireta (IFI, enquanto o HLA-B27 foi tipado em 15 pacientes do grupo I por citometria de fluxo. RESULTADOS: o DNA da C. trachomatis foi evidenciado apenas em uma amostra de líquido sinovial do grupo I (6,7%, sendo o paciente portador de ARe. Em dois pacientes com AR, o DNA clamidial foi identificado na urina (13,3%. Os anticorpos IgG anticlamidiais estavam presentes em oito pacientes da população estudada, três do grupo I (20% e cinco do grupo II (33,3%. O maior título desse anticorpo (1/256 associou-se com a presença do DNA clamidial na urina de um paciente do grupo II. O anticorpo IgM não foi detectado em nenhuma amostra dos dois grupos. O antígeno HLA-B27 foi positivo em quatro indivíduos do grupo II (26,7% e sua presença relacionou-se com sacroileíte. CONCLUSÕES: os resultados deste estudo indicam que em pacientes com diagnóstico de espondiloartropatias e artrite reumatóide, com quadro articular

  11. Ravimid, risk, raha & tervis / Inge Pitsner

    Index Scriptorium Estoniae

    Pitsner, Inge


    Ravimifirma Roche Eesti OÜ tegevjuht Kadri Mägi selgitab ravimiäri toimemehhanisme ning põhjuseid, miks ravimitööstus on riskantne ja kallis valdkond. Lisa: Mis on farmakoökonoomika?; Roche Eesti kontor

  12. Hazardous Waste Cleanup: DSM Nutritional Products Incorporated in Belvidere, New Jersey (United States)

    DSM Nutritional Products, Inc., formerly Hoffman-LaRoche (Roche) Belvidere is located on Maunkachunk Road in White Township, New Jersey. The site occupies approximately 500 acres in Warren County. The facility has been manufacturing vitamins since 1961.

  13. Benefit of hepatitis C virus core antigen assay in prediction of therapeutic response to interferon and ribavirin combination therapy. (United States)

    Takahashi, Masahiko; Saito, Hidetsugu; Higashimoto, Makiko; Atsukawa, Kazuhiro; Ishii, Hiromasa


    A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor test, version 2 (Amplicor M), to estimate the predictive benefit of a sustained viral response (SVR) and non-SVR in 44 genotype 1b patients treated with interferon (IFN) and ribavirin. HCV core Ag negativity could predict SVR on day 1 (sensitivity = 100%, specificity = 85.0%, accuracy = 86.4%), whereas RNA negativity could predict SVR on day 7 (sensitivity = 100%, specificity = 87.2%, accuracy = 88.6%). None of the patients who had detectable serum core Ag or RNA on day 14 achieved SVR (specificity = 100%). The predictive accuracy on day 14 was higher by RNA negativity (93.2%) than that by core Ag negativity (75.0%). The combined predictive criterion of both viral load decline during the first 24 h and basal viral load was also predictive for SVR; the sensitivities of Lumipulse-Ag and Amplicor-M were 45.5 and 47.6%, respectively, and the specificity was 100%. Amplicor-M had better predictive accuracy than Lumipulse-Ag in 2-week disappearance tests because it had better sensitivity. On the other hand, estimates of kinetic parameters were similar regardless of the detection method. Although the correlations between Lumipulse-Ag and Amplicor-M were good both before and 24 h after IFN administration, HCV core Ag seemed to be relatively lower 24 h after IFN administration than before administration. Lumipulse-Ag seems to be useful for detecting the HCV concentration during IFN therapy; however, we still need to understand the characteristics of the assay.

  14. Usefulness of in-house PCR methods for hepatitis B virus DNA detection. (United States)

    Portilho, Moyra Machado; Baptista, Marcia Leite; da Silva, Messias; de Sousa, Paulo Sérgio Fonseca; Lewis-Ximenez, Lia Laura; Lampe, Elisabeth; Villar, Livia Melo


    The aim of the present study was to evaluate the performance of three in-house PCR techniques for HBV DNA detection and compare it with commercial quantitative methods to evaluate the usefulness of in-house methods for HBV diagnosis. Three panels of HBsAg reactive sera samples were evaluated: (i) 50 samples were examined using three methods for in-house qualitative PCR and the Cobas Amplicor HBV Monitor Assay; (ii) 87 samples were assayed using in-house semi-nested PCR and the Cobas TaqMan HBV test; (iii) 11 serial samples obtained from 2 HBV-infected individuals were assayed using the Cobas Amplicor HBV test and semi-nested PCR. In panel I, HBV DNA was detected in 44 samples using the Cobas Amplicor HBV test, 42 samples using semi-nested PCR (90% concordance with Cobas Amplicor), 22 samples using PCR for the core gene (63.6% concordance) and 29 samples using single-round PCR for the pre-S/S gene (75% concordance). In panel II, HBV DNA was quantified in 78 of the 87 HBsAg reactive samples using Cobas TaqMan but 52 samples using semi-nested PCR (67.8% concordance). HBV DNA was detected in serial samples until the 17th and 26th week after first donation using in-house semi-nested PCR and the Cobas Amplicor HBV test, respectively. In-house semi-nested PCR presented adequate concordance with commercial methods as an alternative method for HBV molecular diagnosis in low-resource settings. Copyright © 2015 Elsevier B.V. All rights reserved.

  15. Temperature effect on the poro-mechanical or hydraulic behaviour of a carbonated rock and a mortar: experimental studies; Influence de la temperature sur le comportement poromecanique ou hydraulique d'une roche carbonatee et d'un mortier: etudes experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Lion, M


    The main objective of this study is to evaluate the temperature effect on the hydraulic and poro-mechanical behaviour of a limestone. Many experimental tests (porosity and permeability measurements, uniaxial and hydrostatic compressions tests) were carried out in order to study the thermal treatments effect and so the thermal microcracking effect on rock behaviour. Moreover, an experimental device for permeability measurements under high temperatures (until 200 C) was realized. This experimental device permitted to study the permeability variation of the limestone under thermal stresses. Finally, the behaviour of cementitious materials was studied; the temperature effect on the permeability of a mortar was examined. (author)

  16. Relations entre les types de dépôts évaporitiques et la présence de couches riches en matière organique (roches-mères potentielles Relationship Between Different Types of Evaporitic Deposits and the Occurrence of Organic-Rich Layers (Potential Source Rocks

    Directory of Open Access Journals (Sweden)

    Busson G.


    Full Text Available La fertilité exceptionnelle des eaux salées est confirmée par des études récentes sur l'Actuel (marais salants de Méditerranée occidentale. Le benthos est représenté par des mollusques, foraminifères, ostracodes et surtout des cyanophycées et des populations bactériennes ; le plancton par le microphytoplancton (Dunaliella, diatomées, etc, par le zooplancton (flagellés, Arternia salina et par de très nombreuses bactéries hétérotrophes. Si les espèces sont d'autant plus rares que la salinité est élevée, la prolifération des formes adaptées peut atteindre des valeurs supérieures à celles réalisées dans la plupart des autres milieux. On rappelle ensuite l'efficacité des systèmes d'eaux stratifiées pour la conservation de la matière organique originellement élaborée dans l'eau photique et oxygénée. Ces systèmes peuvent s'accompagner de proliférations bactériennes photosynthétiques exposées à des mortalités en masse pouvant être responsables de véritables lamines organiques. Dans les évaporites de plates-formes où la ségrégation des salinités et des dépôts a été synchrone et latérale, la tranche d'eau a dû être faible et, de ce fait, peu favorable à l'établissement de systèmes d'eaux stratifiées et surtout à leur pérennité géologique. Ces accumulations s'avèrent donc en général pauvres en matière organique, de même qu'elles ne s'accompagnent pas d'appareils récifaux abondants. Dans les évaporites de centre de cuvette, les dépôts sont imputables à une succession de phases de salinité croissante dans le temps : calcaires sur les zones hautes, contemporains de minces couches à matière organique dans les dépressions ; sulfate de calcium sur les aires d'épaisseur moyenne pouvant être encore contemporain de couche à matière organique dans les aires déprimées ; enfin, remplissage par la halite des zones centrales profondes et déprimées. A ces phases successives, se superposent - au moins au cours de la formation des carbonates, puis des sulfates - des gradients de salinité verticaux (système d'eaux stratifiées. Cartographiquement, ces dépôts ont une polarité salin croissant centripète . Quand elles sont situées en contexte carbonaté, ces évaporites de centre de cuvette s'accompagnent fréquemment d'appareils récifaux pouvant fourmiller dans toute une ceinture annulaire (cf. Salina du Silurien du Michigan. Grâce aux minces couches à matière organique des zones déprimées, grâce aux appareils récifaux et grâce à la couverture salifère, ces bassins ont souvent une certaine prolificité en hydrocarbures. Le troisième type correspond aux évaporites de marge de bassin. Dans ce dispositif, le centre du bassin est resté affamé - voire insatiable - pendant de longues périodes. La sédimentation y est restée marine, souvent caractérisée par la seule présence du plancton et du necton et par une richesse plus ou moins permanente en matière organique. Autour de la cuvette s'organisent, sur des plates-formes marginales parfois fort étendues, des appareils construits (bancs ou récifs, puis des faciès lagunaires, carbonatés et évaporitiques et enfin des détritiques continentaux. Les dépôts montrent une polarité salin croissant centrifuge irréductiblement opposée à la polarité inverse des dépôts de centre de cuvette. Le déficit en les sels les plus solubles est remarquable voire considérable, impliquant le reflux de saumures en profondeur à partir de ces annexes évaporatoires qu'ont dû constituer ces plates-formes d'arrière-récifs. L'appel d'eau à partir de ces annexes évaporatoires n'a pu qu'amener un afflux permanent de nutriments dans tout le bassin. Le reflux des saumures quant à lui a favorisé la pérennité d'un système à deux corps d'eau. Grâce à cet ajustement et à la pérennité du système, la prolificité de tels bassins en matière organique a pu être extrêmement importante. Les évaporites de marge de bassin avec couches à matière organique au centre correspondent à une période prolongée. C'est l'essentiel de la vie du bassin. Le remplissage de la cuvette centrale par des évaporites de centre de bassin peut être un épisode bref et qui peut entraîner la disparition même du bassin. Les évaporites de plate-forme s'étalant indifféremment sur l'ancien domaine du bassin comme sur celui des anciennes plates-formes marginales peuvent être l'aube d'un nouveau cycle sédimentaire, indifférent au passé. The extraordinary fertility of saline waters has been confirmed by recent studies of salterns in the western Mediterranean. The benthos contains mollusks, foraminifers, ostracodes and especially Cyanophyceae and bacterial populations. Plankton includes microphytoplankton (Dunaliella, diatoms, etc. , zooplankton (flagellates, Artemia salina and numerous heterotrophic bacteria. Where diversity is low when salinity is high, the proliferation of well adapted forms can be greater than the productivity levels observed in most other environments. The effectiveness of stratified water bodies for the preservation of organic matter originally produced in photic and oxygenated water is brought out. Such stratified systems may be accompanied by the proliferation of photosynthetic bacteria that are exposed to sporadic mass mortality, resulting in the formation of organic laminae at the bottom. In shelf (or epeiric evaporites, where the segregation of salinities and deposits has been synchronous and lateral, the water depth must have been shallow and hence unsuitable for the formation of stratified water bodies and especially for their geological duration. Such accumulations thus generally have a low organic content, and they also do not have abundant reef systems. In basin-center evaporites, the deposits are attributed to a succession of phases of increasing salinity in time, i. e. limestone in high areas, contemporaneous with thin organic-rich layers in low areas (hollows, calcium sulfate in medium-thickness areas which may also be contemporaneous with organicrich layers in low areas, followed by infilling by halite in deep and low-lying central areas. Superposed on these successive phases - at least during the formation of carbonates then sulfates - are vertical salinity gradients (stratified water system. When mapped, such deposits have a centripetal increasing saline polarity. When they are situated in a carbonate context, such basin-center evaporites are often associated with reef systems that may have thrived as a ring-like belt (cf. Silurian Salina, Michigan. Because of the thin organic-rich layers in low-lying areas, reef systems and the salt overburden, such basins are often prolific with hydrocarbons. The third type of evaporitic deposits is represented by basin-margin evaporites. In such a system, the basin center remains starved - even insatiable - for long periods of time. Sedimentation remains marine, often characterized by the presence of plankton and nekton alone and by a more or less constantly high organic content. Around the basin, marginal shelves are built and are even sometimes quite wide, accompagnied by built-up systems (banks or reefs, then by lagoonal, carbonate and evaporitic facies, and finally by continental detritals. The deposits have a centrifugal increasing saline polarity that is irreducibly opposed to the reverse polarity of the basin-center deposits. The deficit in the most soluble salts is significant, even considerable, involving brine outflow downward from these evaporitic appendages that must have been formed by the back-reef shelves. The inflow of water from these evaporitic appendages can only be constantly accompanied by nutrients throughout the entire basin. The outflow of brines enhances the continuing existence of a system with two water bodies. Because of this adjustment and the durable nature of the system, the prolificity of such basins in organic matter may have been extremely high. The basin margin evaporites with organic-rich in the center indicate that the process was a long one. This is the main life of the basin. The filling of the central part by basin-center evaporites may be a brief episode and may even result in the very disappearance of the basin. The shelf evaporites spreading on both the former basin area and the former marginal shelves may be the start of new sedimentary cycle without any connection to the past.

  17. Experimental study of chemical-mechanical coupling during percolation of reactive fluid through rocks under stress, in the context of the CO{sub 2} geological sequestration; Etude experimentale du couplage chimie-mecanique lors de la percolation d'un fluide reactif dans des roches sous contrainte, dans le contexte de la sequestration geologique du CO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Le Guen, Y


    CO{sub 2} injection into geological repositories will induce chemical and mechanical instabilities. The study of these instabilities is based on experimental deformation of natural rock samples under stress, in the presence of fluids containing, or not, dissolved CO{sub 2}. Triaxial cells used for the experiments permitted an independent control and measurement of stress, temperature, fluid pressure and composition. Vertical strains were measured during several months, with a resolution of 1.10{sup -12} s{sup -1} on the strain rate. Simultaneously, fluids were analysed in order to quantify fluid-rock interactions. For limestone samples, percolation of CO{sub 2}-rich fluids increases strain rate by a factor 1.7 up to 5; on the other hand, sandstone deformation remained almost the same. Increase in strain rate with limestone samples was explained by injected water acidification by the CO{sub 2} which increases rock solubility and reaction kinetics. On the opposite, small effect of CO{sub 2} on quartz explains the absence of deformation. X-ray observations confirmed the importance of rock composition and structure on the porosity evolution. Numerical simulations of rock elastic properties showed increasing shear stress into the sample. Measured deformation showed an evolution of reservoir rocks mechanical properties. It was interpreted as the consequence of pressure solution mechanisms both at grains contacts and on grain free surfaces. (author)

  18. Remblai en argiles et limons à silex traités à la chaux, situés en zone inondable. Caractérisation des matériaux. Déviation de Montoire Les Roches-L'Evêque RD917




    Les remblais de la déviation de Montoire (CG41) sont dans la partie inondable de la vallée du Loir. Ils ont été réalisés en argiles à silex et limons traités systématiquement à 1% de chaux vive jusqu'au niveau des plus hautes eaux. Une instrumentation spécifique avec des sondes TDR a permis de suivre l'évolution des teneurs en eau volumiques sur plus d'un an après la réalisation du remblai. Les résultats font l'objet d'un rapport à part. Des investigations complémentaires par carottage ...

  19. Numerical modeling of damage and permeability interaction in rocks. Application for the radioactive waste storage in deep galleries; Modelisation numerique du couplage entre l'endommagement et la permeabilite des roches. Application a l'etude des ouvrages de stockage souterrain

    Energy Technology Data Exchange (ETDEWEB)

    Maleki, K


    The relation between damage and permeability in rocks is a very important subject in industrial applications. It is for example the case of cracks around the radioactive waste storage galleries (EDZ) which can rise considerably the permeability and so make a serious problem for the sealing and the safety of these structures. The same phenomena can occur in the surrounding concrete wall of the nuclear power stations and also in the cracking of the oil-bearing rocks reservoirs. The experimental research on this subject, specially in laboratory G.3S-LMS in Ecole Polytechnique made it possible to determine the orders of damage effect on the permeability change. But a numerical modeling of these phenomena for setting a constitutive behavior law was to be done. This is the principal objective of this thesis. In this project, at first the notion of crack damage is defined. This type of damage is modeled by the disc shaped crack distribution in the 3D space. The disc's geometrical characteristics (radius, direction and opening) obey the statistical distribution laws, depending on the type of loading (compression or extension). The upper and lower limits of the characteristics are fixed according to actual observations, carried out on clay-stone (host rock selected for the realization of an underground research laboratory in Bure). In order to modeling the damage in the porous media, the double porosity concept is considered. A method of homogenization is used to simulate the flow through the network of cracks and porosity. It allows to derive the equivalent permeability of the fractured porous media. The study of the correlations between permeability and damage, obtained by this method for various values of fracture density, made it possible to obtain a relation between permeability and crack damage, for classical types of loading as simple extension and simple compression. To generalize this relation in the case of any specified triaxial loading, the crack damage is put in relation with the notion of mechanical damage, resulting from a mechanical model connecting the damage to stress and strain. Finally, the poro-mechanical behavior law with damage is implemented to the finite elements computer program ESAR-LCPC The modeling of an underground gallery proved that damage calculation by this method is feasible and allows to compute the evolution of the permeability and the variation of the flow entering the gallery as a function of this damage. (author)

  20. Reduction of the greenhouse effect by geological mineral in-situ sequestration of CO{sub 2} in basic rocks: bibliographic synthesis and possibilities in France. Final report; Reduction de l'effet de serre par sequestration geologique minerale in-situ de CO{sub 2} au sein de roches basiques: synthese bibliographique et revue des potentialites en France. Rapport final

    Energy Technology Data Exchange (ETDEWEB)

    Marechal, J.C.; Lachassagne, P


    The report constitutes a first bibliographic study defining the environments the most adapted to the geological mineral in-situ sequestration of CO{sub 2}. For each environment the lithology and the rocks permeability and porosity are analyzed. Thus the possible rocks and deposits in France are presented. (A.L.B.)

  1. Modèle de foration fondé sur le couplage des effets de destruction de la roche et d'évacuation des déblais (bilan solide A Drilling Model Based on Coupling Between Rock Crushing Effects and Bottom Hole Hydraulic Cleaning of Cuttings (Solid Balance

    Directory of Open Access Journals (Sweden)

    Putot C.


    Full Text Available Une première partie rappelle les progrès accomplis dans la représentation du phénomène de forabilité et l'insuffisance des modèles actuels négligeant la dimension hydraulique. L'analyse des diagraphies de poids, couple et vitesse de pénétration doit être revue et l'éclairage apporté par la représentation proposée semble de nature à résoudre certaines difficultés d'interprétation. L'ambiguïté soulevée par la présence simultanée de deux mécanismes possibles de colmatage, l'un naissant au contact de l'outil, l'autre au fond du trou, est évoquée. La deuxième partie concerne la description proprement dite du modèle. Il s'agit d'un schéma préliminaire, assorti d'hypothèses physiques dont certaines pourront paraître grossières. Son intérêt est de montrer qu'avec des considérations rudimentaires mais intuitivement satisfaisantes, l'essentiel du phénomène est décrit et notamment l'allure du processus de bourrage tel que le présente D. Close (1993 Ce schéma aux hypothèses assez restrictives appelle donc des améliorations de tout ordre qui seront abondamment commentées. The first part reviews the progress made in representing the phenomenon of drillability and the insufficiency of current models that neglect the hydraulic dimension. The analysis of weight on bit logs, torque and rate of penetration must be revised, and the insight provided by the proposed representation seems to be of help in solving various interpretation difficulties. The ambiguity created by the simultaneous presence of two possible plugging mechanisms - one stemming from the contact of the bit and the other from the bottom of the borehole - is dealt with. The second part describes the model itself. It is a preliminary sketch combined with physical hypotheses, some of which may seem approximate. Its importance lies in showing that, with rudimentary but intuitively satisfactory considerations, the essential of the phenomenon is described, and especially the rate of the balling-up process as described by D. Close (1993. This outline with fairly restrictive hypotheses thus calls for improvements of all sorts, which are abundantly commented on.

  2. The natural seismic hazard and induced seismicity of the european HDR (hot dry rock) geothermal energy project at Soultz-sous-Forets (Bas-Rhin, France); Alea sismique naturel et sismicite induite du projet geothermique europeen RCS (roche chaude seche) de Soultz-sous-Forets (Bas-Rhin, France)

    Energy Technology Data Exchange (ETDEWEB)

    Helm, J.A.


    Development of the Soultz-sous-Forets HDR (Hot Dry Rock) geothermal energy project will involve important fluid injections which will induce micro-seismic events. This thesis discusses the natural seismicity of the region and induced seismicity associated with fluid injections. A catalogue of all historical and instrument seismicity of the Soultz-sous-Forets (SSF) region has been compiled. This seismicity does not correspond to movements along the major tectonic features of the region. The area around SSF has been identified as being one where high heat flow corresponds to low seismicity. The largest well documented seismic event in the region which took place in 1952 had an epicentral intensity of VI. All important data pertaining to the series of seismic events which took place in the region from August to October 1952 have been collected and are presented. This work details the installation and operation of a permanent 3 station network of accelerometers and seismometers around the HDR site. Also the installation and operation of a mobile network of vertical seismometers during fluid injections. 167 micro-seismic events were recorded on the surface network, with magnitudes from -0.5 to 1.9. The preferential alignment of the micro-seismic cloud is N160 deg. Individual focal mechanisms of the larger seismic events correspond to an extensional tectonic regime. Stress inversion of P wave polarities indicates that the maximum stress is vertical and the intermediate and minimum stress axes horizontal. The largest of the horizontal stresses is orientated N124 deg and the smallest N34 deg. Induced seismic movement is taking place on pre-existing fractures controlled by the in situ stress seismic movement is taking place on pre-existing tectonic fractures controlled by the in situ stress field, and the largest of the induced events had a magnitude 1.9. This level of seismicity does not pose any environmental hazard to the region around Soultz-sous-Forets. (author) 151 refs.

  3. Relations entre les types de dépôts évaporitiques et la présence de couches riches en matière organique (roches-mères potentielles) Relationship Between Different Types of Evaporitic Deposits and the Occurrence of Organic-Rich Layers (Potential Source Rocks)


    Busson G.


    La fertilité exceptionnelle des eaux salées est confirmée par des études récentes sur l'Actuel (marais salants de Méditerranée occidentale). Le benthos est représenté par des mollusques, foraminifères, ostracodes et surtout des cyanophycées et des populations bactériennes ; le plancton par le microphytoplancton (Dunaliella, diatomées, etc), par le zooplancton (flagellés, Arternia salina) et par de très nombreuses bactéries hétérotrophes. Si les espèces sont d'autant plus rares que la salinité...

  4. Non-Invasive Screening Techniques for Drugs of Abuse, (United States)


    Method of detection in biological specimens: RIA, EMIT. PRESCRIPTION DRUGS Barbi turates Hypnotics. Often implicated as the causative or secondary... barbi - turate assay 2.43i by ROCHE and 0% by SYVA; (c) for the amphetamine assay 14.24% by ROCHE and 14.58% by SYVA. The ROCHE and SYVA amphe- tamine...the scientific evolution of causes and effect , and philosophically to the notion that concepts are predictions of facts to be found and consequences

  5. Clinical value of IS6110-based loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis complex in respiratory specimens. (United States)

    Aryan, Ehsan; Makvandi, Manoochehr; Farajzadeh, Ahmad; Huygen, Kris; Alvandi, Amir-Hooshang; Gouya, Mohammad-Mehdi; Sadrizadeh, Ali; Romano, Marta


    A fundamental to global tuberculosis (TB) control is timely and accurate diagnosis of infectious cases of the disease. Among various methods, techniques based on nucleic acid amplification are the ones with promising prospects. The present study evaluates the diagnostic value of the recently developed IS6110-based loop-mediated isothermal amplification (LAMP) for detection of Mycobacterium tuberculosis complex (MTBC) in sputum specimens. In this cross-sectional study (2008-2009), IS6110-LAMP was evaluated on 101 sputum specimens from 93 highly suspected TB patients and compared to Amplicor MTB test and in-house IS6110-PCR and -nested PCR assays. Culture results or clinical recovery following anti-TB therapy was considered as a reference to prove the TB cases. The overall sensitivity of IS6110-LAMP, Amplicor, nPCR, and PCR were respectively 89.6% (69/77 specimens; 95% confidence interval [CI], 80.5-95.4%), 76.6% (59/77 specimens; CI, 65.6-85.5%), 79.2% (61/77 specimens; CI, 68.5-87.6%) and 59.7% (46/77 specimens; CI, 47.9-70.8%). The specificity and positive predictive value (PPV) were 100% for all the tests, and the negative predictive value (NPV) of IS6110-LAMP, Amplicor, nPCR, and PCR were respectively 75%, 57.1%, 60%, and 43.6%. There was an excellent overall agreement between LAMP and nPCR (k 0.828), and between LAMP and Amplicor (k 0.746), in addition to a better tolerance of IS6110-LAMP to inhibitors present in clinical specimens. The better diagnostic performance of IS6110-LAMP compared to Amplicor (p = 0.009), nPCR (p = 0.013) and PCR (p < 0.0001) besides its rapidity, simplicity, and cost-effectiveness makes it a valuable method for the detection of MTBC in clinical samples, particularly in resource-limited settings. Copyright © 2013 The British Infection Association. Published by Elsevier Ltd. All rights reserved.

  6. 75 FR 39024 - Determination That ACCUTANE (Isotretinoin) Capsules, 10 Milligrams, 20 Milligrams, and 40... (United States)


    ... Milligrams, 20 Milligrams, and 40 Milligrams, Were Not Withdrawn From Sale for Reasons of Safety or...), 20 mg, and 40 mg, were not withdrawn from sale for reasons of safety or effectiveness. This...) Capsules, 10 mg, 20 mg, and 40 mg, are the subject of NDA 18-662, held by Hoffman-La Roche, Inc. (Roche...

  7. 12 Layout PPP adjusted.pmd

    African Journals Online (AJOL)


    âge. Pan-africain et se caractérise par une association de roches plutoniques et volcaniques diversifiées. L'étude géochimique des éléments majeurs, des éléments traces et des terres rares montre l'appartenance des roches à la série ...

  8. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences. Joëlle Roche. Articles written in Journal of Chemical Sciences. Volume 121 Issue 4 July 2009 pp 471-479. Biological activities of substituted trichostatic acid derivatives · Cédric Charrier Joëlle Roche Jean-Pierre Gesson Philippe Bertrand · More Details Abstract Fulltext PDF.

  9. Deoxynukleosidkinase aus insektenzellen zur Nukleosidmonophosphat synthese

    DEFF Research Database (Denmark)


    The patent application together with Roche on synthesis of monophosphate nucleotides by Dm-dNK, an enzyme isolated and the corresponding gene cloned in our laboratory.......The patent application together with Roche on synthesis of monophosphate nucleotides by Dm-dNK, an enzyme isolated and the corresponding gene cloned in our laboratory....

  10. Rheumatoid Case with HCV Infection


    Bita Behnava; Seyed-Moayed Alavian


    Case Presentation:A 46-year-old woman referred to our center due to abnormality in aminotransferase level during check up. She had a history of blood transfusion 12 years ago. Anti-HCV Ab by ELISA method and HCV RNA by RT-PCR were positive. HCV RNA by Amplicor HCV monitor test counted 800,000 IU/ml and the genotype was 3a by Specific Primer-Targeted Region Core method. Laboratory evaluation revealed: Hb 11.9 mg/dl, WBC 5000 /ml, platelet count 190,000/ ml, ALT 70 IU/ml, AST 65 IU/ml, Alk phos...

  11. Relationship between formation of HCV-bearing immune complexes and response to inteferon therapy for chroic hepatitis C


    眞鍋, 良二


    We retrospectively analyzed the relationship between formation of HCV-bearing immune complexes (HCV-IC) and response to interferon (IFN) therapy for chronic hepatitis C (CH-C). Five CH-C patients with complete response (CR) to IFN therapy and pre-treatment HCV RNA titer measured by Amplicor HCV-monitor and five stage (biopsy proved fibrosis of the liver)-matiched CH-C patients who were non-esponders (NR) to IFN therapy were enrolled. Pretretment sera were separated into HCV-IC (supernatant) a...

  12. Behaviour of REE during thermal metamorphism and hydrothermal infiltration associated with skarn and vein-type tyngstene ore bodies in Central Morocco


    Giuliani, Gaston; Cheilletz, A.; Mechiche, M.


    Cet article présente une étude géochimique des terres rares de roches et de minerai du gisement du tungstène du djebel Aouam (Maroc central). La distribution des lanthanides dans les roches sédimentaires ne subit aucune modification importante au cours du méthamorphisme thermal. Par contre, les roches affectées par le méthamorphisme hydrothermal présentent d'importantes variations dans la composition en lanthanides. L'analyse de la distribution des terres rares montre que les fluides minérali...

  13. Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil Baixa sensibilidade da reação em cadeia da polimerase para o diagnóstico de meningite tuberculosa no sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Vânia Maria Sabadoto Brienze


    Full Text Available Two polymerase chain reaction (PCR protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively, when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively, in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.Dois protocolos de reação em cadeia da polimerase (PCR apresentaram baixa sensibilidade (36% e 53%, respectivamente, para TB AMPLICOR e PCR aninhado baseado no gene MPB64, quando comparados aos métodos microbiológicos clássicos (73% e 54% respectivamente para baciloscopia e cultura, no diagnóstico de meningite tuberculosa em 91 pacientes do sudeste do Brasil. Somente três pacientes apresentaram PCR positiva e microbiologia negativa. A análise de amostras seqüenciais de líquor com a PCR aninhada detectou DNA de Mycobacterium tuberculosis até 29 dias após a introdução de tratamento.

  14. Presidential Green Chemistry Challenge: 2000 Greener Synthetic Pathways Award (United States)

    Presidential Green Chemistry Challenge 2000 award winner, Roche Colorado, developed a greener synthesis for gancyclovir (Cytovene, a potent antiviral drug) that uses a second-generation Guanine Triester (GTE) process.

  15. On the origin of X-ray variability of SS 433 (United States)

    Band, D. L.; Grindlay, J. E.


    The X-ray flares observed from the central source in SS 433 by the Einstein telescope are attributed to surges in the mass transfer rate due to changes in the critical Roche volume of the companion. Analysis of the Roche potential for a primary with spin misaligned with the orbital axis, as required by the slaved disk model, predicts that the critical Roche volume will contract twice per orbit if the orbit is circular. A critical Roche volume fractional change of 1-2 percent is found by applying this potential to SS 433. The nutation of the companion should not affect the steady precession of its spin. Aspects of this work strengthen the evidence that the compact object might be a black hole.

  16. Expression of polyhedrin-hEGF fusion protein in cultured cells and ...

    African Journals Online (AJOL)



    , protease K, X-gal and related reagents were purchased from Roche ..... fusion protein in silkworm larvae infected with recombinant Bombyx mori nuclear polyhedrosis virus. J. Gen. Virol. 68: 2599-2606. Massotte D (2003).

  17. Tere tulemast tagasi, uue põlvkonna utopistid! / Veronika Valk

    Index Scriptorium Estoniae

    Valk, Veronika, 1976-


    Francois Roche ja Eva Castro tulevikuarhitektuuri käsitlevatest ettekannetest Tallinna arhitektuuribiennaali raames toimunud sümpoosionil. Maketinäitus "Viini eksiilid. Helisev arhitektuur" Heliose kinos. Eksponeeritakse Viini Die Angewandte arhitektuurikoolis (juht Hani Rashid) õppivate eestlaste loomingut

  18. ORIGINAL ARTICLES Routinely available cotrimoxazole ...

    African Journals Online (AJOL)


    . However, because of ... routine provision of CTM prophylaxis to all infants born to. HIV-infected women. We have data ... were tested using a quantitative assay of HIV viral RNA using polymerase chain reaction (PCR, Roche ...

  19. 75 FR 43557 - Notice of Determinations Regarding Eligibility To Apply for Worker Adjustment Assistance (United States)


    .... 73,514 Edward W. Daniel, Cleveland, OH. LLC. 73,779 Portland Title Beaverton, OR. Group, Fidelity... Hoffman-LaRoche, Inc Nutley, NJ. 74,330 San Francisco Union City, CA. Chronicle. 74,338 Madison County...

  20. 76 FR 59416 - Senior Executive Service Performance Review Board (United States)


    ... Goode, Brendan Gowadia, Huban Grade, Deborah C. Gramlick, Carl Graves, Margaret Griffin, Robert P., Ph.D..., Perry J. Robles, Alfonso Roche, William Rogers, Debra Rossides, Gale D. Russell, Anthony Rynes, Joel...

  1. 78 FR 44577 - Senior Executive Service Performance Review Board (United States)


    ... Gersten, David Gina, Allen Goode, Brendan Gowadia, Huban Grade, Deborah C. Gramlick, Carl Graves, Margaret.... Roche, William W. Rodriguez, Waldemar Rogers, Debra Rudolph, Alan Ruppel, Joanna Russell, Anthony A...

  2. Tähelepanu keskmes on viiruslik hepatiit / Marika Kusnets

    Index Scriptorium Estoniae

    Kusnets, Marika


    Ülemaailmsel hepatiidipäeval 19. mail korraldasid Eesti Gastroenteroloogide Selts (EGS) ja Roche Eesti OÜ meediakanalite esindajatele ümarlaua, et aidata teadvustada C-hepatiidi ohtu, testimis- ja ravivõimalusi Eestis

  3. The Report of the Working Group Concerning the Deterrence of and Response to Incidents of Sexual Assault at the U.S. Air Force Academy

    National Research Council Canada - National Science Library


    On January 2, 2003, Dr. James G. Roche, the Secretary of the Air Force, received an e-mail directed initially to female cadets, which asserted that there was a significant sexual assault problem at the United States Air Force...


    African Journals Online (AJOL)

    Merck) respectively. Suspect- ed positive colonies were purified on Brain-Heart Infu- sion plates (BHI) (Difco) and single colonies were trans- ferred to BHl-slants. Identification was carried out by means of the "Enterotube" method (Roch6), followed.

  5. Attentively listening to the speech

    CERN Multimedia

    CERN PhotoLab


    C.J. Zilverschoon, Peter Standley, Christian Roche, Jacques Blondel, Susan Neboux-Bland, Pierre Marti, Jurgen Knott; Roberto Cappi, Pierre Tetu, Charles Rufer, A. L'Aire, Patrick Lienard, Julienne de Winter

  6. Sequence Read Archive (SRA) (United States)

    U.S. Department of Health & Human Services — The Sequence Read Archive (SRA) stores raw sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome...

  7. Collisional Fragmentation Is Not a Barrier to Close-in Planet Formation

    Energy Technology Data Exchange (ETDEWEB)

    Wallace, Joshua; Tremaine, Scott [Department of Astrophysical Sciences, Princeton University, Princeton, NJ 08544 (United States); Chambers, John, E-mail: [Department of Terrestrial Magnetism, Carnegie Institution for Science, Washington, DC 20015 (United States)


    Collisional fragmentation is shown to not be a barrier to rocky planet formation at small distances from the host star. Simple analytic arguments demonstrate that rocky planet formation via collisions of homogeneous gravity-dominated bodies is possible down to distances of order the Roche radius ( r {sub Roche}). Extensive N -body simulations with initial bodies ≳1700 km that include plausible models for fragmentation and merging of gravity-dominated bodies confirm this conclusion and demonstrate that rocky planet formation is possible down to ∼1.1 r {sub Roche}. At smaller distances, tidal effects cause collisions to be too fragmenting to allow mass buildup to a final, dynamically stable planetary system. We argue that even differentiated bodies can accumulate to form planets at distances that are not much larger than r {sub Roche}.

  8. O&P (Ova and Parasite) Test (United States)

    ... Hardy, D. (2002 January). Diarrhea Caused by Parasites. Clinical Laboratories Newsletter, Univ of Roch Med Center, Vol. 22, ... org. Giardia Antigen by EIA. ARUP's Guide to Clinical Laboratory Testing (CLT) [On-line information]. Available online at ...

  9. Comportement différé des marnes du tunnel d'Arbus (France ...

    African Journals Online (AJOL)


    2Laboratoire de Mécanique des Roches, Ecole Polytechnique Fédérale de Lausanne, Suisse. Soumis le : 26.03.2013. Révisé le : 26.01.2014 ... contrainte et à l'action de l'eau. L'historique des chargements et la présence d'eau ... Les géomatériaux et les roches en particulier ont un comportement mécanique complexe qui.

  10. Application de la méthode de balayage d'activité de processus ...

    African Journals Online (AJOL)

    AKA Boko

    performances de la carrière de « Ben Azzouz » en optimisant le nombre de camions nécessaires et les délais de .... le transport de la roche vers le concasseur primaire, le concassage de la roche pour produire le granulat. ..... VARIABLE. MaxLoadedHoles (Maximum de trou pouvant être chargé) nHolesPerShot. VARIABLE.

  11. MODULAR ANALYTICS: A New Approach to Automation in the Clinical Laboratory


    Horowitz, Gary L.; Zaman, Zahur; Blanckaert, Norbert J. C.; Chan, Daniel W.; Dubois, Jeffrey A.; Golaz, Olivier; Mensi, Noury; Keller, Franz; Stolz, Herbert; Klingler, Karl; Marocchi, Alessandro; Prencipe, Lorenzo; McLawhon, Ronald W.; Nilsen, Olaug L.; Oellerich, Michael


    MODULAR ANALYTICS (Roche Diagnostics) (MODULAR ANALYTICS, Elecsys and Cobas Integra are trademarks of a member of the Roche Group) represents a new approach to automation for the clinical chemistry laboratory. It consists of a control unit, a core unit with a bidirectional multitrack rack transportation system, and three distinct kinds of analytical modules: an ISE module, a P800 module (44 photometric tests, throughput of up to 800 tests/h), and a D2400 module (16 photometric tests, throughp...

  12. Acoustic Moorings for Integrated Cetacean-Prey Studies (United States)


    Chou E, Roch MA (in prep) Habitat use of calling blue and fin whales in Southern California Bight. Marine Ecology Progress Series. Wiggins SM...merging acoustics, biology, and oceanography to answering a suite of ecological questions. RELATED PROJECTS The proposed moorings will lead to...Johnson S, Kerosky S, Roche L, Solsona Berga A, Wakefield L, Wiggins SM (2012) Passive Acoustic Monitoring for Marine Mammals in the Gulf of Alaska

  13. adaptation et selection de varietes performantes de riz pluvi

    African Journals Online (AJOL)


    est formée cette roche et de sa constitution chimique. De façon générale, les concentrations en uranium sont faibles dans les roches .... entre 4 et 8 m du niveau de l'eau de mer actuel à l'estuaire de cette rivière [8,9]. Après plusieurs années de ...

  14. Application des méthodes statistiques et géostatistiques à l'étude de ...

    African Journals Online (AJOL)

    Les formations géologiques sont constituées de roches magmatiques (granites, migmatites, gneiss) et métamorphiques (schistes, grès, roches vertes). ... La méthodologie appliquée est fondée sur une approche pluridisciplinaire intégrant les analyses statistiques et géostatistiques, avec un échantillon de 193 forages.

  15. De geologie van den Cimone di Margno en den Monte di Muggio

    NARCIS (Netherlands)

    Buning, W.L.


    Le terrain étudié (fig. 1) est formé de roches métamorphiques pour plus de la moitié; le reste est formé de sédiments variant du Permien au Trias Inférieur, et de roches appartenant à la culmination éruptivocristalline du Cimone di Margno. Le relief du paysage présente en général des pentes douces.

  16. An Interlingual-based Approach to Reference Resolution (United States)


    the basis of morphosyntax as the f-structure is being constructed. Ontological concepts of similar or related type may be called forth during the...buyout (see Appendix I for the original text and its translation into English ). It is taken from the ARPA Machine Translation Evaluation corpus...inversiones realizadas totalizaron 223 millones de pesetas. English Translation of Text 93-1 Roche Buys Docteur Andreu The Roche Group acquired the

  17. A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos

    Directory of Open Access Journals (Sweden)

    Fernando L. GONÇALES JR


    RIBA-2 positive subjects, in 37.5% of the indeterminate RIBA-2 donors and in 9% of the negative RIBA-2 donors. Chronic hepatitis has also been observed in 50% of the histopathological exams of the anti-HCV EIA-2 reagent donors which were indeterminate RIBA-2. Among 18 blood donors with minimal changes histopathological exam 11 (61% were HCV-RNA positive. Our blood donors anti-HCV reagent generally had clinical, laboratorial and histopathological features observed in patients with chronic HCV hepatitis and a high proportion could be identified in interviews and medical evaluation realized in blood blanks. Generally, these HCV infected donors are identified and discharged only by the serological tests results.Entre 1992 e 1997 foram avaliados, ambulatorialmente, 790 doadores de sangue com teste anti-HCV EIA-2 fortemente reagente (relação entre a densidade ótica da amostra / "cut-off" > 3, que haviam sido detectados na triagem sorológica do banco de sangue. Todos eram negativos para doença de Chagas, sífilis, hepatite B (HBsAg e AIDS. Amostras de sangue foram coletadas, na primeira consulta ambulatorial, para a realização de hemograma, exames bioquímicos e novos testes sorológicos para a HVC (anti-HCV EIA-2. Em 226 doadores anti-HCV EIA-2 repetidamente reagentes, realizou-se o teste suplementar de "immunoblot" para a HVC (RIBA-2. Em 209 doadores, pesquisou-se a presença do RNA do VHC pelo teste do PCR, através de exame automatizado (HCV-AMPLICOR, ROCHE. A ultra-sonografia abdominal foi realizada em 366 doadores e a biópsia hepática em 269 concordantes. Notou-se que 95,6% eram EIA-2 repetidamente reagentes, 94% eram assintomáticos e que apenas 2% referiram icterícia pregressa. Em 47% detectou-se, pelo menos, um fator de risco para a transmissão do VHC, sendo o uso de drogas E.V. o principal deles (27,8%. A transfusão de sangue foi o segundo fator na transmissão da HVC (27,2%. Hepatomegalia foi encontrada em 54%. Esplenomegalia e sinais de hipertens

  18. Hydrocarbons in mother rock in France. Initial report and complementary report (further to the law of the 13 July 2011 creating the national commission for orientation, follow-up and assessment of techniques of exploration and exploitation of liquid and gaseous hydrocarbons); Les hydrocarbures de roche-mere en France. Rapport initial et Rapport complementaire (suite a la loi du 13 juillet 2011 creant la Commission nationale d'orientation, de suivi et d'evaluation des techniques d'exploration et d'exploitation des hydrocarbures liquides et gazeux)

    Energy Technology Data Exchange (ETDEWEB)

    Leteurtrois, Jean-Pierre; Durville, Jean-Louis; Pillet, Didier; Gazeau, Jean-Claude; Bellec, Gilles; Catoire, Serge


    These reports aimed at studying the opportunities of development of mother-rock hydrocarbons as well as the associated economic opportunities and geopolitical challenges, exploitation techniques (efficiency, capacity of the French industry, impacts, costs, perspectives), their social and environmental challenges (notably with respect to such a development in France), and legal, regulatory and tax framework. These issues are addressed in the first report whereas the complementary report gives an overview of the evolution of the energy context, of hydrocarbon resources and technologies, of the main actors in the world, and of experiments in France

  19. Systematic review of the performance of HIV viral load technologies on plasma samples. (United States)

    Sollis, Kimberly A; Smit, Pieter W; Fiscus, Susan; Ford, Nathan; Vitoria, Marco; Essajee, Shaffiq; Barnett, David; Cheng, Ben; Crowe, Suzanne M; Denny, Thomas; Landay, Alan; Stevens, Wendy; Habiyambere, Vincent; Perrins, Jos; Peeling, Rosanna W


    Viral load (VL) monitoring is the standard of care in developing country settings for detecting HIV treatment failure. Since 2010 the World Health Organization has recommended a phase-in approach to VL monitoring in resource-limited settings. We conducted a systematic review of the accuracy and precision of HIV VL technologies for treatment monitoring. A search of Medline and Embase was conducted for studies evaluating the accuracy or reproducibility of commercially available HIV VL assays. 37 studies were included for review including evaluations of the Amplicor Monitor HIV-1 v1.5 (n = 25), Cobas TaqMan v2.0 (n = 11), Abbott RealTime HIV-1 (n = 23), Versant HIV-1 RNA bDNA 3.0 (n = 15), Versant HIV-1 RNA kPCR 1.0 (n = 2), ExaVir Load v3 (n = 2), and NucliSens EasyQ v2.0 (n = 1). All currently available HIV VL assays are of sufficient sensitivity to detect plasma virus levels at a lower detection limit of 1,000 copies/mL. Bias data comparing the Abbott RealTime HIV-1, TaqMan v2.0 to the Amplicor Monitor v1.5 showed a tendency of the Abbott RealTime HIV-1 to under-estimate results while the TaqMan v2.0 overestimated VL counts. Compared to the Amplicor Monitor v1.5, 2-26% and 9-70% of results from the Versant bDNA 3.0 and Abbott RealTime HIV-1 differed by greater than 0.5log10. The average intra and inter-assay variation of the Abbott RealTime HIV-1 were 2.95% (range 2.0-5.1%) and 5.44% (range 1.17-30.00%) across the range of VL counts (2log10-7log10). This review found that all currently available HIV VL assays are of sufficient sensitivity to detect plasma VL of 1,000 copies/mL as a threshold to initiate investigations of treatment adherence or possible treatment failure. Sources of variability between VL assays include differences in technology platform, plasma input volume, and ability to detect HIV-1 subtypes. Monitoring of individual patients should be performed on the same technology platform to ensure appropriate interpretation of

  20. [Low rate of oropharyngeal human papillomavirus infection among women with cervical lesion. Preliminary results from the South-Eastern Hungarian population]. (United States)

    Vanya, Melinda; Jakó, Mária; Terhes, Gabriella; Szakács, László; Kaiser, László; Deák, Judit; Bártfai, György


    Although the natural history of cervical and oral human papillomavirus infection has been intensively investigated in the past years, the ability of this virus to infect oral and genital mucosae in the same individual and its potential to co-infect both cervical and oral mucosa are still unclear. The aim of the authors was to assess the presence of oropharyngeal human papillomavirus infection in women with cervical lesions in the South-Eastern Hungarian population. The total of 103 women have been included in the study between March 1, 2013 and January 1, 2015. Brushing was used to collect cells from the oropharyngeal mucosa. Human papillomavirus DNA was detected using polymerase chain reaction, and Amplicor line blot test was used for genotyping. Oropharyngeal human papillomavirus infection was detected in 2 cases (3%). The detected genotypes were 31, 40/61 and 73 in the oropharyngeal region. The results indicate that in women with cervical lesions oropharyngeal human papillomavirus infection rarely occurs.

  1. CMV DNA e MRNA Pp67 nel monitoraggio del paziente sottoposto a trapianto di midollo osseo

    Directory of Open Access Journals (Sweden)

    Maria Bonaria Goffi


    Full Text Available Primary infection of immunocompetent individuals does not lead to complication, but CMV is a major clinical problem in transplant recipients.Thus, it is important to use sensitive and specific diagnostic techniques to rapidly and accurately detect CMV infection and identify patients at risk of developing CMV disease. In the present study, CMV infection after bone marrow transplantation was monitored by two molecular biology assays: COBAS AMPLICOR CMV MONITOR and NUCLISENS CMV m-RNA pp67. CMV m-RNApp67 assay was found to show positivity later than CMV-DNA MONITOR. The increment of number of copies/ml agreed with the clinical symptoms of CMV infection. The quantitative results of the CMV MONTOR assay was more helpfull to select an antiviral strategy than the qualitative results of NUCLISENS m-RNA pp67 assay.

  2. Development of a TaqMan assay for the six major genotypes of hepatitis C virus: Comparison with commercial assays

    DEFF Research Database (Denmark)

    Engle, Ronald E; Russell, Rodney S; Purcell, Robert H


    A quantitative real-time PCR assay was developed that detects genomic RNA from reference strains representing the six major genotypes of hepatitis C virus (HCV) with equal sensitivity and accurately measured HCV RNA in JFH1 HCV-infected Huh7.5 cells. The method is indirectly calibrated to the first...... international (WHO 96/790) HCV standard preparation and has a linear dynamic range of 10(2.6)-10(6.5) IU/ml. In addition, the inter- and intra-assay precision were approximately 3% CV and HCV RNA Nucleic Acid Technology kits...... (Versant HCV RNA 3.0 b-DNA and Amplicor HCV Monitor), that also employ the WHO standard, allowed validation of the TaqMan assay against all major HCV genotypes. Both commercial methods detected HCV RNA over a wide dynamic range, but showed a consistent difference of about 0.3 log10 when evaluating samples...

  3. Collisional Fragmentation Is Not a Barrier to Close-in Planet Formation (United States)

    Wallace, Joshua; Tremaine, Scott D.; Chambers, John E.


    Collisional fragmentation is shown to not be a barrier to rocky planet formation at small distances from the host star. Simple analytic arguments demonstrate that rocky planet formation via collisions of homogeneous gravity-dominated bodies is possible down to distances of order the Roche radius. Extensive N-body simulations that include plausible models for fragmentation and merging of gravity-dominated bodies confirm this conclusion and demonstrate that rocky planet formation is possible for orbits down to about 1.1 times the Roche radius. At smaller distances, tidal effects cause collisions to be too fragmenting to allow mass build-up to a final, dynamically stable planetary system. We argue that even differentiated bodies can accumulate to form planets at distances that are not much larger than the Roche radius.

  4. Origine de la minéralisation des eaux des aquifères discontinus ...

    African Journals Online (AJOL)

    ... représentés dans ces eaux. Les principaux ions à l'origine de la minéralisation des eaux sont issus de l'altération des roches, de l'hydrolyse des minéraux silicatés tels que l'anorthite dans les plagioclases et de la décomposition des minéraux ferro-magnésiens comme la biotite et l'amphibole présentes dans les roches.

  5. Duas novas espécies de Ptychoderes Schoenherr (Coleoptera, Anthribidae, Anthribinae, Ptychoderini Two new species of Ptychoderes Schoenherr (Coleoptera, Anthribidae, Anthribinae, Ptychoderini

    Directory of Open Access Journals (Sweden)

    José Ricardo M. Mermudes


    Full Text Available Duas novas espécies de Ptychoderes Schoenherr, 1823 são descritas e ilustradas: Ptychoderes magnus sp. nov., da Bolívia e Brasil (localidade-tipo: Ouro Preto d'Oeste, Rondônia e P. jekeli sp. nov. do Brasil e Guiana Francesa (localidade-tipo: Roches de Kourou.The following new species of Ptychoderes Schoenherr, 1823 are described and illustrated (type-localities parenthetic: Ptychoderes magnus sp. nov., from Bolivia and Brazil (Rondônia: Ouro Preto d'Oeste and P. jekeli sp. nov., from Brazil and French Guiana (Roches de Kourou.

  6. Influence de la structure des gneiss et des granites sur la resistance ...

    African Journals Online (AJOL)

    A partir de la structure des roches (gneiss et granites) provenant des différentes régions du Togo il a été proposée une classification basée sur le modèle exponentielle appliquée à la probabilité de présence de grains de dimensions variables. Cette classification a permis d'évaluer l'homogénéité granulaire des roches de ...

  7. Inhibition of Ovarian Cancer by microRNA-mediated Regulation of Telomerase (United States)


    progression, ovarian cancer 16. SECURITY CLASSIFICATION OF: 17. LIMITATION OF ABSTRACT 18. NUMBER OF PAGES 19a. NAME OF RESPONSIBLE PERSON USAMRMC a...studies charac- terizing the 13q14 deletion in human chronic lymphocytic leukemia , which revealed two miRNAs, miR-15a and miR-16-1 (3). Subsequently...Roche) for 1 h and incubated with anti-DIG-AP Fab fragments (Roche) for 12 h. Following wash for three times in 1 maleic acid and 0.3% Tween 20 buffer

  8. New patterns in dust off the edge of Saturn’s main rings (United States)

    Chancia, Robert Ormal; Hedman, Matthew M.; Ye, Shengyi; Kurth, William S.


    The Roche Division is a 3000-km-span of diffuse dust located at the outer edge of Saturn’s main ring system between the A and F rings. Several high-resolution images from Cassini’s orbit insertion revealed two regions with higher concentrations of dust within the Roche Division. These proposed dusty ringlets R/2004 S1 (radius=137,630 km) and R/2004 S2 (radius=138,900 km) lie coincident with the orbit of Atlas and slightly interior to the orbit of Prometheus respectively (Porco et al. 2005a Science). Using several image sequences later obtained by Cassini Hedman et al. 2009 (Icarus) found that these concentrations of dusty ring material are not simple ringlets, but are in fact organized into canted azimuthal brightness variations with a periodicity akin to a 3:4 resonance with Saturn’s rotation rate. The presumed perturbing phenomenon is Saturn’s kilometric radiation (SKR), a strong low wavelength component of the planet’s radio emissions, whose power oscillates near the planet’s rotation rate at a frequency matching the Roche Division structures. However, over the course of the Cassini mission the SKR period has varied by at least 7 minutes. As a result, the location of the resonance moved interior to the edge of the A ring and out of the Roche Division around the time of Saturn’s equinox. Subsequent observations of the Roche Division in the this time period show no evidence of the prominent structures previously observed during the first few years of the Cassini mission. Recently, the 3:4 resonant structures have reappeared in the Roche Division as one of the SKR periods has increased. Here we show that the these dusty Roche Division structures are present in sync with the varying SKR period using images sequences spanning the entire Cassini mission. We also highlight some particularly optimal observations, obtained during Cassini’s F-ring proximal and grand finale orbits, which reveal the dusty structure of the Roche Division in unprecedented

  9. Karst et ressources en eau au moyen Atlas nord-oriental


    Taous, Ali; Tribak, Abdellatif; Obda, Khalid; Baena Escudero, Rafael; López Lara, Enrique Javier; Miranda Bonilla, Jose


    Le Moyen Atlas nord-oriental se caractérise par des paysages kartisques hérités et actuels très diversifiés. En fait, les roches carbonatées du Jurassique, bien représentées dans cette région montagneuse, ont subi une karstification ancienne, depuis la fin du Tertiaire et au cours du Quaternaire. Actuallement, la karstogenèse est encore active en raison des conditions géologiques, morphostructurales et bioclimatiques favorables: couches de roches carbonatées inclinées et plissées, climat médi...

  10. The use of coded PCR primers enables high-throughput sequencing of multiple homolog amplification products by 454 parallel sequencing

    DEFF Research Database (Denmark)

    Binladen, Jonas; Gilbert, M Thomas P; Bollback, Jonathan P


    the high-throughput Genome Sequence 20 DNA Sequencing System (GS20, Roche/454 Life Sciences). Each DNA sequence is subsequently traced back to its individual source through 5'tag-analysis. CONCLUSIONS: We demonstrate that this new approach enables the assignment of virtually all the generated DNA sequences...

  11. [Comparison of efficiency and cytotoxicity of different transfection reagents in transfecting RIP140-siRNA into Kupffer cells]. (United States)

    Li, Ji; Liu, Zuojin


    To compare the efficiency and cytotoxicity of different transfection reagents used in transfection of RIP140-siRNA into Kupffer cells to optimize the transfection conditions. Kupffer cells were transfected with RIP140-siRNA labeled with GFP as the reporter gene using lipofectamine 2000, Roche reagent (X-treme GENE siRNA Transfection Reagent) and puro screening lentivirus (1.0×10(8) TU/mL) as the transfection reagents. The transfection effect was observed under a fluorescent inverted microscope, and laser scanning confocal microscopy was used to analyze RIP140 expression in trasnfected Kupffer cells. Flow cytometry was performed to detect cell apoptosis, and CCK-8 test was used to evaluate the cell proliferation inhibition. RT-RCR and Western blotting were performed to detect the expressions of RIP140 mRNA and protein in the trasnfected cells. Puro screening lentivirus yielded the highest cell transfection efficiency, which exceeded 90%, followed by Roche reagent and then by lipofectamine 2000. Flow cytometry and CCK-8 test showed that the cytotoxicity was the mildest with Roche reagent, moderate with lentivirus, and severe with lipofectamine 2000. The cells trasnfected with lentivirus showed a significantly lower RIP140 expression than cells trasnfected with lipofectamine 2000 and Roche reagent (Ptransfection, as compared with the other two trasnfection reagents, can achieve good transfection efficiency with a relativelty low cytotoxicity, and allows for better controllability and stability of the trasnfectiion conditions.

  12. Molecular characterization and expression of the GDF9 gene in New ...

    Indian Academy of Sciences (India)

    Caixia Sun


    Jun 17, 2017 ... chosen and sent to Shanghai Biological Engineering for sequencing. The obtained sequences and putative amino acid sequences were aligned by DNAMAN. qPCR analysis. The qPCR was used to measure the mRNA expression pattern of the GDF9 gene in different tissues on a Roche. LightCycler. ®.

  13. Assessment of Recent Process Analytical Technology (PAT) Trends: A Multiauthor Review

    DEFF Research Database (Denmark)

    Simon, Levente L.; Pataki, Hajnalka; Marosi, György


    in industrial laboratories and in manufacture e.g. at GSK, AstraZeneca and Roche. Furthermore, the paper discusses the PAT paradigm from the regulatory science perspective. Given the multidisciplinary nature of PAT, such an endeavour would be almost impossible for a single author, so the concept...

  14. Seeking a Human Spaceflight Program Worthy of a Great Nation. Review of U.S. Human Spaceflight Plans Committee (United States)


    Roche Harrison “Jack” Schmitt John Schumacher John Shannon Brewster Shaw Milt Silvera S. Fred Singer George Sowers Jim Spann Paul Spudis Steve Squyres...Regolith Shielding”, NASA Johnson Space Center, 14 July 2009 Spann , James, “Science from the Moon”, NASA Marshall Space Flight Center, 9 July 2009

  15. ORIGINAL ARTICLES PMTCT from research to reality - results from ...

    African Journals Online (AJOL)

    8.7% at 6 weeks and 8.9% at 3 months of age itt the sttidy population verifies the high rate of NVP administration ... PCR (Roche Molecular Systems, Basel, Switzerland) at 6 weeks and 3 months of age and an HIV ELISA at 12 .... have the advantage of strengthening the country's general health care service." The infant HIV ...

  16. Isolation and drought-tolerant function analysis of ZmPti1-1, a ...

    African Journals Online (AJOL)



    Jun 15, 2011 ... Zhiliang Li1,2,3, Mingdi Bian2, Zhongyi Wu2, Xiuhai Zhang2, Qing Yang1, and Conglin Huang2*. 1College of Life ... drought stress, compared with wild type, survival rate of the three transgenic lines, which was 70, 76 and 87% .... detection starter kit II, Roche Corporation, Basel, Switzerland). The ethidium ...

  17. Pharmaceutical lobbying and pandemic stockpiling of Tamiflu: a qualitative study of arguments and tactics. (United States)

    Vilhelmsson, Andreas; Mulinari, Shai


    Little is known about how pharmaceutical companies lobby authorities or experts regarding procurement or the use of vaccines and antivirals. This paper investigates how members of Denmark's pandemic planning committee experienced lobbying efforts by Roche, manufacturer of Tamiflu, the antiviral that was stockpiled before the 2009 A(H1N1) pandemic. Analysis of interviews with six of seven members of the Danish core pandemic committee, supplemented with documentary analysis. We sought to identify (1) arguments and (2) tactics used in lobbying, and to characterize interviewees' views on the impact of (3) lobbying and (4) scientific evidence on the decision to stockpile Tamiflu. Roche lobbied directly (in its own name) and through a seemingly independent third party. Roche used two arguments: (1) the procurement agreement had to be signed quickly because the drug would be delivered on a first-come, first-served basis and (2) Denmark was especially vulnerable to an influenza crisis because it had smaller Tamiflu stocks than other countries. Most interviewees suspected that lobbying had an impact on Tamiflu procurement. Our study highlights risks posed by pharmaceutical lobbying. Arguments and tactics deployed by Roche are likely to be repeated whenever many countries are negotiating drug procurements in a monopolistic market. © The Author 2017. Published by Oxford University Press on behalf of Faculty of Public Health.

  18. Anticonvulsants for Nerve Agent-Induced Seizures: The Influence of the Therapeutic Dose of Atropine (United States)

    2007-01-01 Sidell, 1989; Aas, 2003). However, i.m. diazepam produces doi:10.1 124/jpet.106.111252. inconsistent bioavailability , it is slow to act, and it...Scientific (Hampton, NH). PB, diazepam, and midazolam were obtained from Hoffman-La Roche Inc. ( Nut - Model A -30 min 0 11 min Onset +5 min ley, NJ), and

  19. Chapter 3: Evaluation of the OvineSNP50 genotyping array in four ...

    African Journals Online (AJOL)

    Sandenbergh, Lise


    Mar 21, 2016 ... 2010; Kijas et al., 2012). Approximately 500 SNPs discovered through BAC end Sanger sequencing of nine animals, each representing a different breed, are incorporated in the 50K chip. Roche 454 whole-genome shotgun sequencing of six individuals of six different breeds provided approximately 33 000 ...

  20. Browse Title Index

    African Journals Online (AJOL)

    Bruno Léopold Agboton, Jacques Vigan, Simon Azonbakin, Roch Sotindjo, Patricia Yêkpê, Marius Adjagba, Bonaventure Awede, Raphaël Darboux, Anatole Laleye. Vol 15, No 1 (2013), Polymorphisme de l'apolipoprotéine E dans la population du nord du Maroc: fréquence et influence sur les paramètres lipidiques ...

  1. Journal of Astrophysics and Astronomy | Indian Academy of Sciences

    Indian Academy of Sciences (India)


    Jan 27, 2016 ... Kopal (Adv. Astron. Astrophys., 9, 1, 1972) introduced the concept of Roche equipotentials to analyse the effects of rotational and tidal distortions in case of stars in binary systems. In this approach a mathematical expression for the potential of a star in a binary system is obtained by approximating its inner ...

  2. Dual function of the hemagglutinin H5 fused to chicken CD154 in a ...

    African Journals Online (AJOL)

    Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study. AG Pose, ES Rodriguez, AC Mendez, JN Gomez, AV Redondo, ER Rodriguez, EMG Ramos, AA Gutierrez, MPR Molto, DG Roche, YS Ugalde, AM Lopez ...

  3. SNP Discovery In Marine Fish Species By 454 Sequencing

    DEFF Research Database (Denmark)

    Panitz, Frank; Nielsen, Rasmus Ory; van Houdt, Jeroen K J


    Based on the 454 Next-Generation-Sequencing technology (Roche) a high throughput screening method was devised in order to generate novel genetic markers (SNPs). SNP discovery was performed for three target species of marine fish: hake (Merluccius merluccius), herring (Clupea harengus) and sole...

  4. Maternal and perinatal outcome of patients with severe pre ...

    African Journals Online (AJOL)


    quatorze femmes ont reçu du. MgSO tandis ... 2006 and received Roche brand of Diazepam as infusion of 20mg in 500mls of intravenous fluid over 6 .... is prescribed at the study center in preference to Pritchard(18) and other regimens because.

  5. Evaluation of a radioimmunoassay (/sup 125/I) kit for cannabinoid metabolites in urine and whole blood

    Energy Technology Data Exchange (ETDEWEB)

    Childs, P.S.; McCurdy, H.H.

    The Abuscreen kit (Roche Diagnostics) for the analysis of 9/-tetrahydrocannabinol-9-carboxylic acid and other cannabinoids in urine was evaluated in terms of its accuracy, reproducibility, and sensitivity. A procedure is also presented for the analysis of total cannabinoids in whole blood using the RIA kit.

  6. Evaluation of NGAL TestTM on Cobas 6000

    DEFF Research Database (Denmark)

    Hansen, Young B L; Damgaard, Anette; Poulsen, Jørgen H


    BACKGROUND: Neutrophil Gelatinase-Associated Lipocalin (NGAL) is a promising biomarker for acute kidney injury (AKI). Our objectives were to evaluate the NGAL Test(TM) from Bioporto for both urine NGAL and plasma NGAL on the Cobas 6000 c501 (Roche Diagnostics, Rotkreuz, Switzerland) with matched...


    African Journals Online (AJOL)


    Roche, A., Girish, B. V., de Baere, T., Baudin, E., Boige, V., Elias, D., Lasser, P., Schlumberger, M., Ducreux,. M., (2003). Trans-catheter arterial chemoembolization as first-line treatment for hepatic metastases from endocrine tumors. Eur Radiol. 13(1): 136-140. 18. Stuart, K., (2003). Chemoembolization in the management of ...

  8. Anti-Neuroinflammatory Effects of Houttuynia cordata Extract on LPS ...

    African Journals Online (AJOL)

    Inflammation in brain disorders is characterized by activation of glial cells (mainly microglia and astrocytes) ... infections, cerebral ischemia, traumatic brain injury, or neuronal/parenchyma damage [1]. Activation of .... Mini, Roche, Manheim, Germany) at 4 °C. The lysate was clarified by centrifugation at 10,000 g for 20 min at ...

  9. Effect of para-chlorophenoxyacetic acid on acid invertase gene ...

    African Journals Online (AJOL)

    Your User Name


    Jul 4, 2011 ... enzymes and the effects of PCPA treatment on gene expression of soluble acid invertase (AI) during tomato fruit development were .... with DIG high prime DNA labelling and detection starter kit II. (Roche). RESULTS. Effect of ... during this time, sucrose content of fruit from the treated plants was slightly ...

  10. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences. Philippe Bertrand. Articles written in Journal of Chemical Sciences. Volume 121 Issue 4 July 2009 pp 471-479. Biological activities of substituted trichostatic acid derivatives · Cédric Charrier Joëlle Roche Jean-Pierre Gesson Philippe Bertrand · More Details Abstract Fulltext ...

  11. Sharing Cookies: A Case Study (United States)

    Salls, Jenny


    Rational number interpretations can include part-whole, measure, ratio, quotient, and operator. These are all subconstructs of partitioning (Barnett-Clarke et al. 2010; Behr et al. 1980; Clarke, Roche, and Mitchell 2008; Flores, Samson, and Yanik 2006). Each of these subconstructs uses different cognitive skills (Driscoll 1984), so it is important…

  12. Efficacy of various single-dose regimens of ceftriaxone in ...

    African Journals Online (AJOL)


    Aug 18, 1990 ... The therapeutic efficacy of single intramuscular doses of ceftriaxone (Rocephin; Roche) (62,S, 125 and 250 mg), admini- stered without probenecid, was evaluated in 167 adult males with uncomplicated acute gonococcal urethritis. Cure rates of 100% were achieved at 62,5 mg and 250 mg. In the 125 mg.

  13. Efficacy of various single-dose regimens of ceftriaxone in ...

    African Journals Online (AJOL)

    The therapeutic efficacy of single intramuscular doses of ceftriaxone (Rocephin; Roche) (62,S, 125 and 250 mg), administered without probenecid, was evaluated in 167 adult males with uncomplicated acute gonococcal urethritis. Cure rates of 100% were achieved at 62,5 mg and 250 mg. In the 125 mg dose group, ...

  14. The genome and transcriptome of perennial ryegrass mitochondria

    DEFF Research Database (Denmark)

    Islam, Md. Shofiqul; Studer, Bruno; Byrne, Stephen


    and annotation of the complete mitochondrial genome from perennial ryegrass. Results: Intact mitochondria from perennial ryegrass leaves were isolated and used for mtDNA extraction. The mitochondrial genome was sequenced to a 167-fold coverage using the Roche 454 GS-FLX Titanium platform, and assembled...

  15. Middle-to-Upper Palaeolithic site formation processes at the Bordes-Fitte rockshelter (Central France)

    DEFF Research Database (Denmark)

    Aubry, Thierry; Dimuccio, Luca Antonio; Buylaert, Jan-Pieter


    . In this article we use the Middle and Early Upper Palaeolithic archaeo-stratigraphic record from the Bordes-Fitte rockshelter (les Roches d'Abilly site, Central France), a Bayesian analysis of the ages obtained by accelerator mass spectrometry radiocarbon on ultrafiltered collagen and by luminescence on quartz...

  16. Mitigation of TGF-β/Smad signaling pathway-associated liver fibrosis ...

    African Journals Online (AJOL)

    control group received equivalent volume of corn oil (CCL4 vehicle), at the same frequency in which CCL4 was administered to the test rats. The study protocol was ... determined by automated analyzer (Roche,. Indianapolis, USA). Quantitative polymerase chain reaction. (qPCR) assay. qPCR was performed with SsoFast ...

  17. 503-IJBCS-Article-Prof Ag Michele Ake

    African Journals Online (AJOL)


    Vitamine B1. In. Les Vitamines: Données Biochimiques,. Nutritionnelles et Cliniques. Roche: Paris; 107-142. Majumdar SK, Shaw GK, O'Gorman P, Aps. EJ, Offermann EL, Thomson AD. 1982. Blood vitamin status (B1, B2, B6, folic acid and B12) in patients with alcoholic liver disease. Int. J. Vit. Nutr. Res., 52(3):. 266 - 271.

  18. Adams operations in smooth K-theory


    Bunke, Ulrich


    We show that the Adams operations in complex K-theory lift to operations in smooth K-theory. The main result is a Riemann-Roch type theorem about the compatibility of the Adams operations and the integration in smooth K-theory.

  19. Polymerase Chain Reaction (PCR) Detection Of The Genome Of ...

    African Journals Online (AJOL)

    A single, discrete and specific band of expected size (278bp) when measured against 200bp (base pair) DNA molecular marker (Roche) was observed from all the three ... This communication herein reported is the first documented report of the detection of ASFV from a Nigerian warthog reported hitherto only in eastern and ...

  20. Oral midazolam in paediatric premedication | Payne | South African ...

    African Journals Online (AJOL)

    In a premedication study involving 135 children, aged 1 - 10 years, four regimens were investigated: (I) no premedication; (il) oral trimeprazine tartrate 2 mg/kg, methadone 0,1 mg/kg, droperidol 0,15 mg/kg (TMD); (iil) intramuscular midazolam . (Dormicum; Roche) 0,15 mg/kg; and (iv) oral midazolam 0,45 mg/kg.

  1. Les Basaltes de l’Atlas de Béni-Mellal (Haut Atlas Central, Maroc : un Volcanisme Transitionnel Intraplaque Associé aux Stades de L’évolution Géodynamique du Domaine Atlasique

    Directory of Open Access Journals (Sweden)

    Jaouad Guezal


    Full Text Available Les roches basaltiques de l’Atlas de Béni-Mellal (Haut-Atlas Central, Maroc, mises en place, dans les différents bassins synclinaux de la région, se manifestent en deux horizons volcaniques B1 et B2. Les deux événements éruptifs sont d’âge Jurassique moyen pour les coulées de l’horizon B1 et d’âge Crétacé inférieur pour celles de l’horizon B2. Les roches sont caractérisées par un faciès des basaltes à olivines. La composition de leurs clinopyroxènes est marquée par deux tendances : augite au niveau des basaltes à olivine du B1 et diopside au niveau de ceux du B2. La composition géochimique sur roche totale dévoile le caractère transitionnel de ces roches. La relative dissimilitude géochimique signalée au niveau des basaltes des deux horizons peut être expliquée par un début du changement de la nature du magmatisme de la région en relation avec l’évolution géodynamique du domaine atlasique.

  2. Multimorbidity in a large district hospital: A descriptive cross ...

    African Journals Online (AJOL)

    Multimorbidity in a large district hospital: A descriptive cross-sectional study. S Roche, E de Vries. Abstract. Background. There is substantial research documenting the burden of disease globally and in the South African (SA) primary care context. Few studies address the disease profile and its implications in the SA hospital ...

  3. Two new species of Metrosideros (Myrtaceae) from New Caledonia: dual characterisation with morphology and nrDNA sequence variation

    NARCIS (Netherlands)

    Dawson, John W.; Whitaker, Anthony H.; Whitaker, Vivienne A.; Gardner, Richard C.; Wright, Shane D.


    We have identified two new species of Metrosideros subg. Mearnsia (Myrtaceae) growing on, and apparently endemic to, the massif known as the Roches d’Ouaïème in New Caledonia. Of these two species, we include M. whitakeri in sect. Mearnsia and M. rotundifolia in sect. Calyptropelala. Both

  4. Author Details

    African Journals Online (AJOL)

    Roche, JR. Vol 34 (2004): Supplement 2 - Articles The paradigm of efficiency and sustainability - a dairying perspective: keynote address. Abstract PDF. ISSN: 2221-4062. AJOL African Journals Online. HOW TO USE AJOL... for Researchers · for Librarians · for Authors · FAQ's · More about AJOL · AJOL's Partners · Terms ...

  5. Prognostic Factors Of Typhoid Perforation in Lagos | Atoyebi ...

    African Journals Online (AJOL)

    Prognostic Factors Of Typhoid Perforation in Lagos. OA Atoyebi, AA Adesanya, CE Atimomo, JT Da Roche- Afodu. Abstract. Perforation of the ileum remains one of the commonest and the most dramatic complication of Typhoid fever in developing countries where the infection is still prevalent. A prospective study of patients ...

  6. Programme intégré de lutte contre le paludisme en Afrique ...

    International Development Research Centre (IDRC) Digital Library (Canada)

    Institution. International Centre of Insect Physiology and Ecology. Institution Country. Kenya. Institution Website. Project Leader. Roch Dabiré. Institution. Centre national de la recherche scientifique et technologique. Institution Country. Burkina Faso. Project Leader. Kato Jonas Njunwa. Project Leader.

  7. assessment of the effect of dihydroartemisinin-sodium valproate ...

    African Journals Online (AJOL)



    Dec 2, 2014 ... and 12 h light/dark cycle; and allowed to acclimatize with the laboratory environment for at least 3 days prior to the commencement of the experiments. Drugs: The following drugs were used: Dihydroartemisinin (Cotecxin- Jiaxing Nanhu China),. Valproate (Epillin- Sanofi UK), Diazepam (Valium-. Roch UK).

  8. A path integral derivation of χy-genus

    International Nuclear Information System (INIS)

    Meng, Guowu


    The formula for the Hirzebruch χ y -genus of complex manifolds is a consequence of the Hirzebruch-Riemann-Roch formula. The classical index formulae for Todd genus, Euler number and signature correspond to the case when the complex variable y=0, -1 and 1 respectively. Here we give a direct derivation of this nice formula based on supersymmetric quantum mechanics

  9. Swedish Defence Research Abstracts 82/83-2 (Froe Foersvars Forsknings Referat 82/83-2). (United States)


    alarm (78) Carbon dioxide retention inside motorcycle helmets (79) Vibration and decompression gas bubbles (80) No effects of nootropic drugs on memory... nootropic drugs on memory and psychomotor performance during reduced air pressure (in English) S. Levander and others Report I from the Roche Clinical Unit

  10. Correction: General optimization procedure towards the design of a new family of minimal parameter spin-component-scaled double-hybrid density functional theory. (United States)

    Roch, Loïc M; Baldridge, Kim K


    Correction for 'General optimization procedure towards the design of a new family of minimal parameter spin-component-scaled double-hybrid density functional theory' by Loïc M. Roch and Kim K. Baldridge, Phys. Chem. Chem. Phys., 2017, 19, 26191-26200.

  11. Download this PDF file

    African Journals Online (AJOL)

    Colonial Masters in Nigeria before Independence. It was partly funded by the Colonial Administrative Government and companies such as John Holt, Royal Exchange Assurance. Corporation, and Pharmaceutical companies such as Glaxo, May. & Baker, Wellcome, Roche, Winthrop and Hoechst. This journal which is often ...

  12. Neutrophil superoxide-anion generating capacity in chronic smoking ...

    Indian Academy of Sciences (India)


    IU (268 mg) vitamin E as dL-α-tocopherol (n = 64) or placebo capsules (n = 64), provided by Hoffman La. Roche Ltd (Basel, Switzerland). Fasting blood samples for analysis of different parameters, were collected before and after the intervention. Written informed con- sent was obtained from all participants, and the protocol.

  13. Download this PDF file

    African Journals Online (AJOL)

    eradication of smallpox and the near» representing stage-specific," 'sp0rozoite's,' eradication of polio [2]. It is surprising that a merozoites, trophozoites and ..... Le Roch KG, Zhou Y, Blair PL, el 01. Discovery of gene function by expression. profiling of the malaria parasite life cycle. Science. 2003; 301:1503-1508. 9. Clyde DF ...

  14. The American cranberry mitochondrial genome reveals the presence of selenocysteine (tRNA-Sec and SECIS) insertion machinery in land plants (United States)

    The American cranberry (Vaccinium macrocarpon Ait.) mitochondrial genome was assembled and reconstructed from whole genome 454 Roche GS-FLX and Illumina shotgun sequences. Compared with other Asterids, the reconstruction of the genome revealed an average size mitochondrion (459,678 nt) with comparat...

  15. Notes on G-theory of Deligne-Mumford stacks


    Toen, B.


    Based on the methods used by the author to prove the Riemann-Roch formula for algebraic stacks, this paper contains a description of the rationnal G-theory of Deligne-Mumford stacks over general bases. We will use these results to study equivariant K-theory, and also to define new filtrations on K-theory of algebraic stacks.

  16. Reference range determination for whole-blood platelet aggregation using the Multiplate analyzer

    NARCIS (Netherlands)

    Peerschke, Ellinor I. B.; Castellone, Donna D.; Stroobants, A. K.; Francis, John


    To develop reference ranges for platelet aggregation using the Multiplate analyzer (Roche Diagnostics, Mannheim, Germany) in blood anticoagulated with sodium citrate (Na-citrate), lithium heparin (Li-heparin), or hirudin. The study was performed at three sites on consented, healthy adults (n = 193)

  17. Progress in the search for the C-CN2 carbene

    Czech Academy of Sciences Publication Activity Database

    Hanzlová, E.; Martinů, T.; Navrátil, R.; Shaffer, Christopher; Schröder, Detlef


    Roč. 106, - (2012), s1295-s1295 ISSN 0009-2770. [EuCheMS Chemistry Congress /4./. 26.08.2012-30.08.2012, Prague] Institutional research plan: CEZ:AV0Z40550506 Keywords : bromodiazirinyl anion * diazirinylidene * nucleophilic ROCH Subject RIV: CF - Physical ; Theoretical Chemistry

  18. Velocity Distributions of Runaway Stars Produced by Supernovae in ...

    Indian Academy of Sciences (India)

    Chunhua Zhu, Zhaojun Wang. & Guoliang Lü. School of Physical Science and Technology, Xinjiang University, Urumqi, 830046, China. .... is critical for the success of SN Ia. For the retention efficiencies of .... the equilibrium radii of donors exceed their Roche lobe by as much as a factor of 2. However, in Wang & Han (2009), ...

  19. Journal of Chemical Sciences | Indian Academy of Sciences

    Indian Academy of Sciences (India)

    Home; Journals; Journal of Chemical Sciences. Cédric Charrier. Articles written in Journal of Chemical Sciences. Volume 121 Issue 4 July 2009 pp 471-479. Biological activities of substituted trichostatic acid derivatives · Cédric Charrier Joëlle Roche Jean-Pierre Gesson Philippe Bertrand · More Details Abstract Fulltext ...

  20. Nonlinear Time Series Analysis via Neural Networks (United States)

    Volná, Eva; Janošek, Michal; Kocian, Václav; Kotyrba, Martin

    This article deals with a time series analysis based on neural networks in order to make an effective forex market [Moore and Roche, J. Int. Econ. 58, 387-411 (2002)] pattern recognition. Our goal is to find and recognize important patterns which repeatedly appear in the market history to adapt our trading system behaviour based on them.

  1. diversidade genética de ovinos naturalizados no brasil

    African Journals Online (AJOL)



    Nov 8, 2012 ... although, in most cases, they are found in intergenic spacer regions. Until recently, the standard techniques of prospecting for SNP markers were based on Sanger's sequencing method, although second-generation se- quencing technologies (Roche 454 - Margulies et al.,. 2005; Solexa-Illumina – Bennett, ...

  2. Education on Correct Inhaler Technique in Pharmacy Schools ...

    African Journals Online (AJOL)

    Basheti IA, Reddel HK, Armour CL, Bosnic-Anticevich. SZ. Counseling about turbuhaler technique: needs assessment and effective strategies for community pharmacists. Respir Care 2005; 50(5): 617-623. 8. Giraud V, Allaert FA, Roche N. Inhaler technique and asthma: feasibility and acceptability of training by pharmacists.

  3. Evaluation of DNA Repair Function as a Predictor of Response in a Clinical Trial of PARP Inhibitor Monotherapy for Recurrent Ovarian Carcinoma (United States)


    applicable; NOVA, Niraparib in Ovarian Cancer; PARP, poly (ADP-ribose) polymerase; SOLO , Studies of Olaparib in Ovarian Cancer; WT, wild type. Ovarian...AstraZeneca Travel , Accommodations, Expenses: Pfizer, Roche, AstraZeneca, Clovis Oncology Other Relationship: Clovis Oncology Elizabeth M. Swisher

  4. The value of serum Hepatitis B surface antigen quantification in ...

    African Journals Online (AJOL)

    HBe status, HBV DNA concentrations and serum ALT levels, based on the European Association for the study of the liver (EASL) guidelines.Serum HBsAg titres were measured using the Elecsys HBsAg II Quant assay (Roche Diagnostics).

  5. The Advantage of Reading over Listening Text Comprehension in Down Syndrome: What Is the Role of Verbal Memory? (United States)

    Roch, Maja; Florit, Elena; Levorato, M. Chiara


    The current study was designed to investigate the role played by verbal memory in the advantage shown by individuals with Down syndrome in reading over listening text comprehension (Roch & Levorato, 2009). Two different aspects of verbal memory were analyzed: processing load and coding modality. Participants were 20 individuals with Down syndrome,…

  6. Effect of dietary vitamin E on the performance of broilers and quality ...

    African Journals Online (AJOL)


    A randomised block design was used in both experiments. The nutrient composition of the experimental diets is given in Table 1. In experiment 1, ten levels of vitamin E (supplied by Hoffmann La-Roche, Basel, Switzerland) were supplemented to the basal diet (Table. 2), resulting in 11 treatments: 1) basal diet (control); 2) ...

  7. Methodologies for Quantitative Systems Pharmacology (QSP) Models: Design and Estimation

    NARCIS (Netherlands)

    Ribba, B.; Grimm, H. P.; Agoram, B.; Davies, M. R.; Gadkar, K.; Niederer, S.; van Riel, N.; Timmis, J.; van der Graaf, P. H.


    With the increased interest in the application of quantitative systems pharmacology (QSP) models within medicine research and development, there is an increasing need to formalize model development and verification aspects. In February 2016, a workshop was held at Roche Pharma Research and Early

  8. NJP VOLUME 42 NO 1

    African Journals Online (AJOL)



    Oct 17, 2014 ... severe malaria, severe malnutrition, diarrhoea amongst others common in the tropics1-4 and may also complicate treatment with some drugs like quinine used in ..... heim (Germany): Roche Diagnos- tics; 2003. 18. Cheesebrough M. District Labora- tory Practice in Tropical Countries,. Part 2. Cambridge: ...

  9. Probing thermonuclear burning on accreting neutron stars

    NARCIS (Netherlands)

    Keek, L.


    Neutron stars are the most compact stars that can be directly observed, which makes them ideal laboratories to study physics at extreme densities. Neutron stars in low-mass X-ray binaries accrete hydrogen and helium from a lower-mass companion star through Roche lobe overflow. This matter undergoes

  10. Characterization of nine new microsatellite loci for the marbled newt ...

    Indian Academy of Sciences (India)

    on a GsFLX PTP (Roche, Basel, Switzerland) following the manufacturer's instructions. One hundred and seventy- three microsatellite sequences were identified. From these, all sequences with an important number of repeats (as they have better chances to be polymorphic) and a size greater than 100 bp were selected.

  11. Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.

    LENUS (Irish Health Repository)

    Jones, S


    Norovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche\\/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche\\/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.

  12. Two paintings by Francisco Bayeu in Vergara

    Directory of Open Access Journals (Sweden)

    José Ignacio Calvo Ruata


    Full Text Available Two unpublished paintings by Francisco Bayeu are presented, located in the church of Saint Marina in Oxirondo (Vergara, Guipúzcoa. Both are dedicated to Saint Roch and Saint Sebastian, as they are two versions of the same subject because the work had to be repeated.

  13. First Hα and Revised Photometric Studies of Contact Binary ...

    Indian Academy of Sciences (India)

    Common among the variable stars are the contact binaries which form an excellent source in determining the stellar parameters with high degree of accuracy (Southworth. 2012). These binaries which are also referred to as W Ursae Majoris (W UMa) stars occur when both components of the system fill their Roche lobes ...

  14. New Automated Hepatitis C Virus (HCV) Core Antigen Assay as an Alternative to Real-Time PCR for HCV RNA Quantification▿ †


    Park, Yongjung; Lee, Jong-Han; Kim, Beom Seok; Kim, Do Young; Han, Kwang-Hyub; Kim, Hyon-Suk


    An automated hepatitis C virus (HCV) antigen (Ag) assay was evaluated with clinical samples. Determination of HCV Ag and RNA levels in 282 subjects using Abbott HCV Ag and Roche Cobas TaqMan assays revealed that these two tests were highly correlated (r = 0.9464). Thus, the HCV Ag assay could be an alternative test to quantitative reverse transcription-PCR.

  15. 75 FR 39335 - Incidental Takes of Marine Mammals During Specified Activities; Marine Seismic Survey in the... (United States)


    ... enforcement of laws and treaties. The Healy is a USCG icebreaker, capable of traveling at 5.6 km/hour (three... (Haley and Ireland, 2006; Haley, 2006; Jackson and DesRoches, 2008; Mosher et al., 2009). The estimated... Breaking Ice Outside of U.S. Waters for the Proposed Arctic Ocean Survey (Haley and Ireland, 2006; Haley...

  16. 76 FR 47210 - Medical Devices; Availability of Safety and Effectiveness Summaries for Premarket Approval... (United States)


    ... total ankle May 27, 2009. Innovations, Inc. replacement system. P060004(S1) FDA-2011-M-0256... Carl.......... Chestnut Medical Pipeline embolization device.... April 6, 2011. Technologies, Inc. P100034 FDA-2011-M-0295...-0300....... Roche Molecular cobas HPV test April 19, 2011. Systems, Inc. P100029 FDA-2011-M-0296...

  17. Genome analysis methods: Theobroma cacao [PGDBj Registered plant list, Marker list, QTL list, Plant DB link and Genome analysis methods[Archive

    Lifescience Database Archive (English)

    Full Text Available Theobroma cacao ... 2n=20 ... 2011 Sanger (WGS), Roche/454 , Illumina 26 Gb 326 Mb 16.7x... Newbler 4,792 25,912 EUGene following specific training for T. cacao 28,798 CIRAD; Dec2010 Dec2010 10.1038/ng.736 21186351 ...

  18. Prevalence of oral and oropharyngeal human papillomavirus in a ...

    African Journals Online (AJOL)

    Male factory workers were recruited. Oral rinse and gargle samples were tested for 37 HPV types using the Linear Array HPV Genotyping Test (Roche Molecular Systems). A questionnaire was used to obtain information regarding age, medical conditions, substance and alcohol use and sexual behaviour. HIV testing was ...

  19. Combining the IADPSG criteria with the WHO diagnostic criteria for ...

    African Journals Online (AJOL)

    glucose, 1‑hour and 2‑hour serum glucose. Serum glucose was analysed on the Roche/Hitachi 902 automatic analyzer. The women were classified as gestational diabetics and non‑gestational diabetic according to the WHO and IADPSG diagnostic criteria. Five pregnancy outcome parameters were observed during the ...

  20. Incorporation of ice sheet models into an Earth System model: focus ...

    Indian Academy of Sciences (India)


    2008, 2010; Robinson et al. 2010; Fyke et al. 2011; Roche et al. 2014; Thoma et al. 2015). Reviewing of them is out of the scope of this paper. We focus on the. 1. 2. 3. 4. 5. 6. 7. 8 .... not in the focus of the research). Assumed ..... Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel.

  1. Model of contract of purchase of the electric power produced by facilities that use the energy of aquifers or of underground rocks and benefiting from the electricity purchase obligation. Established after enforcement of the article 5 of the decree from May 10, 2001 and approved by the minister attended to electricity; Modele de contrat d'achat de l'energie electrique produite par les installations utilisant l'energie des nappes aquiferes ou des roches souterraines et beneficiant de l'obligation d'achat d'electricite. Etablie en application de l'article 5 du decret du 10 mai 2001 et approuve par le Ministre charge de l'electricite

    Energy Technology Data Exchange (ETDEWEB)



    This model of contract comprises 2 parts. The first part describes the general conditions of electric power purchase: aim of the contract, connection to the grid and delivery point, producer's facility, reciprocal commitments and stoppages for maintenance purpose, energy and power metering and control, energy delivery, payment for the purchased power (payment and payment indexing), taxes, payments, contract enforcement, date line, suspension, modification or cancellation, conciliation in case of dispute. A recall of the tariffs mentioned in the by-law from March 13, 2002, of the approximation rules and a model of certificate are given in appendixes. The second part gives some complements to the general conditions (purchaser and producer corporate, characteristics of the facility, details about the connection and delivery point, description of the metering system, tariffs of purchase and indexing, payment of bills, contract characteristics, subscription for a power supply contract). (J.S.)

  2. Verification of the harmonization of human epididymis protein 4 assays. (United States)

    Ferraro, Simona; Borille, Simona; Carnevale, Assunta; Frusciante, Erika; Bassani, Niccolò; Panteghini, Mauro


    Serum human epididymis protein 4 (HE4) has gained relevance as an ovarian cancer (OC) biomarker and new automated methods have replaced the first released manual EIA by tracing results to it. We verified agreement and bias of automated methods vs. EIA as well as possible effects on patients' management. One hundred and fifteen serum samples were measured by Abbott Architect i2000, Fujirebio Lumipulse G1200, Roche Modular E170, and Fujirebio EIA. Passing-Bablok regression was used to compare automated assays to EIA and agreement between methods was estimated by Lin's concordance correlation coefficient (CCC). The bias vs. EIA was estimated and compared to specifications derived from HE4 biological variation. Median (25th-75th percentiles) HE4 concentrations (pmol/L) were 84.5 (60.1-148.8) for EIA, 82.7 (50.3-153.9) for Abbott, 89.1 (55.2-154.9) for Roche, and 112.2 (67.8-194.2) for Fujirebio. Estimated regressions and agreements (95% confidence interval) were: Abbott=1.01(0.98-1.03) EIA-4.8(-7.5/-2.6), CCC=0.99(0.99-1.00); Roche=0.91(0.89-0.93) EIA+5.7(4.2/8.0), CCC=0.98(0.98-0.99); Fujirebio=1.20(1.17-1.24) EIA+ 2.4(-0.6/4.9), CCC=0.97(0.96-0.98). The average bias vs. EIA resulted within the desirable goal for Abbott [-3.3% (-6.1/-0.5)] and Roche [-0.2% (-3.0/2.5)]. However, while for Abbott the bias was constant and acceptable along the measurement concentration range, Roche bias increased up to -28% for HE4 values >250 pmol/L. Lumipulse showed a markedly positive bias [25.3% (21.8/28.8)]. Abbott and Roche assays exhibited a good comparability in the range of HE4 values around the previously recommended 140 pmol/L cut-off. For patient monitoring, however, the assay used for determining serial HE4 must not be changed as results from different systems in lower and higher concentration ranges can markedly differ.

  3. [Clinical benefit of HCV core antigen assay in patients receiving interferon and ribavirin combination therapy]. (United States)

    Higashimoto, Makiko; Takahashi, Masahiko; Jokyu, Ritsuko; Saito, Hidetsugu


    A highly sensitive second generation HCV core antigen assay has recently been developed. We compared viral disappearance and kinetics data between commercially available core antigen assays, Lumipulse Ortho HCV Ag, and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor Test, Version 2 to estimate the predictive benefit of sustained viral response (SVR) and non-SVR in 59 patients treated with interferon and ribavirin combination therapy. We found a good correlation between HCV core Ag and HCV RNA level regardless of genotype. Although the sensitivity of the core antigen assay was lower than PCR, the dynamic range was broader than that of the PCR assay, so that we did not need to dilute the samples in 59 patients. We detected serial decline of core Ag levels in 24 hrs, 7 days and 14 days after interferon combination therapy. The decline of core antigen levels was significant in SVR patients compared to non-SVR as well as in genotype 2a, 2b patients compared to 1b. Core antigen-negative on day 1 could predict all 10 SVR patients (PPV = 100%), whereas RNA-negative could predict 22 SVR out of 25 on day 14 (PPV = 88.0%). None of the patients who had detectable serum core antigen on day 14 became SVR(NPV = 100%), although NPV was 91.2% on RNA negativity. An easy, simple, low cost new HCV core antigen detecting system seems to be useful for assessing and monitoring IFN treatment for HCV.

  4. Chlamydia trachomatis ompA genotypes in male patients with urethritis in Greece: conservation of the serovar distribution and evidence for mixed infections with Chlamydophila abortus. (United States)

    Psarrakos, Panagiotis; Papadogeorgakis, Eleni; Sachse, Konrad; Vretou, Evangelia


    PCR amplification and nucleotide sequencing of the ompA gene of Chlamydia trachomatis were used to determine the prevalence and distribution of genotypes in 51 urine and urethral specimens from Greek male patients with urethritis, that were positive by the COBAS Amplicor test. A single C. trachomatis serovar was identified in 43 of the 51 amplified samples. Serovars F and E were the most prevalent (both 12, 28%), followed by D (9, 21%), G (4, 9%), B and K (both 2, 5%) and H and J (both 1, 2%). Over one third of the samples bared a variant ompA genotype that had been previously identified in other areas worldwide. Two results in this study, both observed for the first time, were of particular interest. First, the emergence of the unique variant genotype D/Ep6 (X77364.2) identified in 3 urethral samples. Second, the ompA genotype OCLH196 of the animal pathogen Chlamydophila abortus as well as a 23S rRNA gene fragment of this species detected by the assay ArrayTube™ was found in 7 urethral samples. The implications resulting from this observation for the health of the general population are discussed. Copyright © 2011 Elsevier Ltd. All rights reserved.

  5. Detection of HIV-1 in Saliva: Implications for Case-Identification, Clinical Monitoring and Surveillance for Drug Resistance§ (United States)

    Balamane, Maya; Winters, Mark A; Dalai, Sudeb C; Freeman, Alexandra H; Traves, Mark W; Israelski, Dennis M; Katzenstein, David A; Klausner, Jeffrey D


    Background: Saliva tests that detect antibodies are used to diagnose HIV infection. The goal of this study was to determine whether saliva could be used for nucleic acid-based tests to measure HIV-1 virus load (VL) and detect drug resistance. Methods: 69 HIV infected individuals provided 5-10 ml of saliva and blood samples. Viral RNA was isolated from saliva and dried blood spots using the Nuclisens extraction. Saliva VL was measured using a modified Amplicor assay, and genotyping was performed using an in-house RT-PCR/sequencing protocol. Plasma VLs were obtained from concurrently drawn clinical tests. Results: Thirty-six of 47 (77%) plasma viremic patients had measurable saliva HIV-1 RNA. Paired plasma and saliva HIV RNA levels were significantly correlated (Spearman’s correlation = .6532, p50 copies/mL had detectable saliva HIV RNA, and the genotypic data was highly concordant between saliva and plasma. In patients with high levels of plasma HIV RNA, saliva might be useful in identifying viremia and evaluating drug resistance. PMID:21673840

  6. Detection of HIV-1 in Saliva: Implications for Case-Identification, Clinical Monitoring and Surveillance for Drug Resistance. (United States)

    Balamane, Maya; Winters, Mark A; Dalai, Sudeb C; Freeman, Alexandra H; Traves, Mark W; Israelski, Dennis M; Katzenstein, David A; Klausner, Jeffrey D


    Saliva tests that detect antibodies are used to diagnose HIV infection. The goal of this study was to determine whether saliva could be used for nucleic acid-based tests to measure HIV-1 virus load (VL) and detect drug resistance. 69 HIV infected individuals provided 5-10 ml of saliva and blood samples. Viral RNA was isolated from saliva and dried blood spots using the Nuclisens extraction. Saliva VL was measured using a modified Amplicor assay, and genotyping was performed using an in-house RT-PCR/sequencing protocol. Plasma VLs were obtained from concurrently drawn clinical tests. Thirty-six of 47 (77%) plasma viremic patients had measurable saliva HIV-1 RNA. Paired plasma and saliva HIV RNA levels were significantly correlated (Spearman's correlation = .6532, p50 copies/mL had detectable saliva HIV RNA, and the genotypic data was highly concordant between saliva and plasma. In patients with high levels of plasma HIV RNA, saliva might be useful in identifying viremia and evaluating drug resistance.

  7. Rapid assessment of sexually transmitted diseases in a sentinel population in Thailand: prevalence of chlamydial infection, gonorrhoea, and syphilis among pregnant women--1996. (United States)

    Kilmarx, P H; Black, C M; Limpakarnjanarat, K; Shaffer, N; Yanpaisarn, S; Chaisilwattana, P; Siriwasin, W; Young, N L; Farshy, C E; Mastro, T D; St Louis, M E


    To determine the prevalence of sexually transmitted diseases (STDs) among pregnant women in Thailand, where case reporting suggests a marked decrease in STDs following a campaign promoting condom use during commercial sex. Cross sectional study of women at their first visit to the study hospitals' antenatal clinics in Chiang Rai (n = 500) and Bangkok (n = 521). First catch urine specimens were tested for Chlamydia trachomatis and Neisseria gonorrhoeae using the Amplicor CT/NG polymerase chain reaction assay. Syphilis and HIV serological testing were performed in the study hospitals' laboratories. The prevalence of chlamydial infection was 5.7%, gonorrhoea 0.2%, and syphilis 0.5% (all VDRL or RPR titres were gestational age at first antenatal clinic visit, but was not associated with marital status, gravidity, city of enrollment, or HIV infection status. There was a low prevalence of gonorrhoea and syphilis among these pregnant women in Thailand. Chlamydial infection was detected at a higher prevalence, especially among younger women and women registering later for antenatal care. Testing of pregnant women using easily collected urine specimens and a sensitive nucleic acid amplification assay is a feasible method of rapidly assessing chlamydial and gonococcal prevalence.

  8. Frequency of very low HCV viremia detected by a highly sensitive HCV-RNA assay. (United States)

    Fytili, P; Tiemann, C; Wang, C; Schulz, S; Schaffer, S; Manns, M P; Wedemeyer, H


    Only limited data is available on the frequency and clinical significance of very low hepatitis C viremia (HCV quantification. We evaluated the new Abbott m2000 RealTime PCR assay in 3213 consecutive anti-HCV-positive sera as well as in 50 HCV-recovered patients with sustained virological response to standard antiviral therapy. The assay showed a linear range between 10(1) IU/ml and 10(7) IU/ml for HCV genotypes 1-6. An HCV viremia below 600 IU/ml was detected more often with the m2000 RealTime PCR assay than with the Cobas Amplicor assay in viremic sera (7.1% versus 1.8%). Seventy-seven cases with HCV levels below 100 IU/ml not related to ongoing antiviral therapy were identified. An HCV-RNA of less than 12 IU/ml was found in nine of the 50 SVR patients. Two patients had a viral load of 34 IU/ml and 84 IU/ml, respectively, one of those showed persistently elevated ALT levels over a period of 5 years after the end of antiviral treatment. An HCV viremia below 600 IU/ml can be detected in almost every 40th anti-HCV-positive sera using real-time PCR based assays. Low persisting HCV-RNA in patients after antiviral therapy may be associated with mild liver inflammation in single cases.

  9. COBAS ® AmpliPrep/COBAS ® Taqman ® HCV Quantitative Test, version 2.0: an in vitro test for hepatitis C virus RNA quantification. (United States)

    Deeks, Emma D


    The COBAS(®) AmpliPrep/COBAS(®) Taqman(®) HCV Quantitative Test, version 2.0, is an in vitro, fully automated, real-time, nucleic acid amplification test indicated for quantifying hepatitis C virus (HCV) RNA levels in the plasma/serum of patients infected with HCV of genotypes 1-6 who are receiving anti-viral therapy. By quantifying levels of HCV RNA in these patients, the test can be used early in the course of treatment to predict the likelihood of a sustained virologic response being achieved and can also be used to assess virologic response during treatment as part of response-guided therapy. The test has excellent sensitivity, high specificity and a broad linear range of quantitation. It correlates well with version 1.0 of the same test, but has the benefit of better sensitivity and genotype inclusivity and a smaller sample input volume. It also correlates well with other available HCV tests, including other quantitative real-time PCR tests (specifically the COBAS(®) Taqman(®) HCV Test, version 2.0, used with the high pure system, and the Abbott Realtime and Artus HCV QS-RGQ tests), the Versant(®) branched DNA quantitative test, the COBAS(®) Amplicor HCV Qualitative PCR Test and the Versant(®) HCV qualitative transcription-mediated amplification assay. The test is not indicated for HCV infection diagnosis or to screen for the presence of HCV in blood/blood products.

  10. [Prevalence of hepatitis C virus and excessive consumption of alcohol in a nonhospital worker population]. (United States)

    Prieto Domingo, J J; Carrión Bolaños, J A; Bandrés Moya, F


    The aim of the study was to know the prevalence of the hepatitis C virus (HCV) in a non hospital work population by ELISA 3.0 and PCR-Amplicor, as well as its relationship with excessive alcohol intake (more than 280 g/week in men and 168 g/week in women). A transversal seroepidemiologic study was carried out in 1,109 workers of the Empresa Nacional de Electricidad, S.A. (ENDESA). During the annual medical examinations (April 1993-October 1994) the amount of alcoholic beverages each worker had consumed over the 7 days prior to the medical examination was obtained by anamnesis together with a blood sample for different laboratory tests. Sixteen percent of the workers had had excessive alcohol intake. The prevalence of anti HCV antibodies in the study population was 2.4% being up to 4.6% in the workers declaring excessive alcohol consumption and 10.4% if they also presented an elevation in any of the transaminases. The prevalence of the potentially ineffective workers was 1.46%. The prevalence of anti C antibodies by ELISA 3.0 was greater than expected (2.4%) significantly increasing in the population group which declared excessive alcohol intake, thereby demonstrating the relationship between alcohol and hepatitis C.

  11. Infrared spectroscopy of symbiotic stars and the nature of their cool components

    International Nuclear Information System (INIS)

    Kenyon, S.J.; Gallagher, J.S.


    We present low-resolution 2--4 μm spectroscopy of a small sample of symbiotic stars, in an effort to determine if the giant components of these systems fill their Roche Lobes. A [2.35]-[2.2] color index measures the strength of the CO absorption band and provides a useful discriminant of luminosity class among single M-type giants which separates normal giants from supergiants at the same spectral type. Although interpretation of symbiotic spectra is complicated somewhat by their binary nature, our results suggest the late-type components in these systems range from normal red giants to bright asymptotic giants. The possible presence of non-Roche Lobe filling, low-luminosity giants in some symbiotic stars cannot be understood within the framework of existing theories for these interesting objects, and thus may provide important information for understanding mass transfer in binary systems

  12. Influence of stellar radiation pressure on flow structure in the envelope of hot-Jupiter HD 209458b (United States)

    Cherenkov, A. A.; Bisikalo, D. V.; Kosovichev, A. G.


    Close-in exoplanets are subjected to extreme radiation of their host stars. Photometric observations of the hot-Jupiter HD 209458b transit by HST/STIS detected strong absorption in the Ly α line, thus indicating the existence of a hydrogen envelope extending beyond the Roche lobe. The gasdynamic modelling (Bisikalo et al.) showed that the stable structure of this envelope is maintained by the balance between the Roche lobe overfilling and stellar wind pressure. Obviously, the dynamics and stability of the envelope can be affected by stellar radiation pressure. Using 3D gasdynamic simulations, we study the impact of radiation pressure in the Ly α line on the envelope of hot-Jupiter HD 209458b, and show that the effect is not strong enough to significantly affect the gasdynamics in the system. For a detectable radiation pressure effect the intensity of the Ly α line has to be by two orders of magnitude greater.

  13. An International Collaborative Study of Outcome and Prognostic Factors in Patients with Secondary CNS Involvement By Diffuse Large B-Cell Lymphoma

    DEFF Research Database (Denmark)

    El-Galaly, Tarec Christoffer; Cheah, Chan Yoon; Bendtsen, Mette Dahl


    that develop SCNS following primary therapy that contains a rituximab-based-regimen as well as the impact of more intensified treatment for SCNS are limited.Aims: The aims of this study were to i) describe the natural history of SCNS in a large cohort of patients treated with immunochemotherapy, and ii......' Hospital, London). Clinico-pathologic and treatment characteristics at the time of SCNS were collected from medical records.Results: In total, 281 patients with SCNS diagnosed between 2001 and 2016 were included. Median age at SCNS was 64 (range 20-93) years and male:female ratio was 1.3. SCNS occurred...... Myers Squib: Research Funding; NanoString Technologies: Research Funding; F Hoffmann-La Roche: Research Funding; Millennium Takeda: Research Funding; Seattle Genetics: Research Funding. Sehn: roche/genentech: Consultancy, Honoraria; amgen: Consultancy, Honoraria; seattle genetics: Consultancy, Honoraria...

  14. Fatty liver change as a result of hepatic artery infusion chemotherapy. (United States)

    Zeiss, J; Merrick, H W; Savolaine, E R; Woldenberg, L S; Kim, K; Schlembach, P J


    Direct hepatic arterial infusion of floxuridine (FUDR) (Roche Laboratories, Division of Hoffman-LaRoche Inc., Nutley, NJ, U.S.A.) has been used extensively in the treatment of metastases of colorectal tumors to the liver. The effectiveness of infusion and tolerance of the chemotherapy has been improved utilizing a totally implantable infusion pump delivery system. However, unequal distribution of drug infusion may occur to different parts of the liver parenchyma as demonstrated by hepatic arterial infusion scintigraphy (HAPS). We present a case of such unequal perfusion in which fatty metamorphosis of the liver occurred in the overperfused liver segment after FUDR therapy. The liver parenchymal changes were followed by serial computerized tomography scans and proven by biopsy during a minilaparotomy. Serial HAPS examinations show redirection of subsequent infusion to the opposite liver lobe due to parenchymal damage and vascular sclerosis. The importance of uniform liver perfusion and a rare dose-related effect of FUDR on liver parenchyma are thereby demonstrated.

  15. Contraction of the solar nebula

    International Nuclear Information System (INIS)

    Rawal, J.J.


    The concept of Roche limit is applied to the Laplacian theory of the origin of the solar system to study the contraction of a spherical gas cloud (solar nebula). In the process of contraction of the solar nebula, it is assumed that the phenomenon of supersonic turbulent convection is operative and brings about the halt at various stages of contraction. It is found that the radius of the contracting solar nebula follows the Titius-Bode law. The consequences of the relation are also discussed. The aim is to attempt to explain, on the basis of the concept of Roche limit, the distribution of planets in the solar system and try to understand the physics underlying it. (Auth.)

  16. Further considerations on contracting solar nebula (United States)

    Rawal, J. J.


    Kepler's third law of the planetary system is analyzed in terms of Prentice's (1978a) modern Laplacian theory and Rawal's (1984) Roche limit concept. Prentice has determined, based on laws of conservation of mass and angular momentum and the concept of supersonic turbulent convection, that the ratio of orbital radii of successively disposed gaseous rings is a constant of approximately 1.69. Rawal studied the relationship between the supersonic turbulent convection concept and Roche limit and established a limit of 1.442. It is concluded that Kepler's law determines the rotation period of the sun at the time of its formation at the present radius to be equal to 0.1216 d. The correlation between orbital periods of the radii and resonant structure is investigated.

  17. Étude hydrogéochimique des eaux des aquifères de fractures du ...

    African Journals Online (AJOL)

    Les eaux souterraines de la région sont bicarbonatées calciques (93,75%) et chlorurées calciques (6,25%). On note dans l'ensemble que les eaux des aquifères de fractures sont sous-saturées en calcite et en dolomite. La minéralisation des eaux souterraines de la région a pour origine, l'altération des roches, l'hydrolyse ...

  18. Orlistat. No hurry....



    Treatments for obesity are disappointing. None has yet shown an effect on morbidity and mortality. Nondrug treatments are poorly assessed. Stable long-term weight loss necessitates long-term management. Orlistat (Xenical, Hoffman-La Roche), a gastrointestinal lipase inhibitor, is indicated, in combination with a low-calorie diet, for management of obesity. The assessment file is bulky and methodologically sound, at least in terms of the weight loss end point. During medium-term trials (12 to ...

  19. Olea europaea Linn (Oleaceae) Fruit Pulp Exhibits ...

    African Journals Online (AJOL)

    of 50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 %. Triton X-100, 0.5 % sodium deoxycholate, 0.1 % sodium dodecyl sulfate (SDS), 1 mM EDTA. (Sigma), 1% protease inhibitor cocktail, and 1 % phosphatase inhibitor cocktail (Roche). After centrifugation, the protein concentration of the supernatant was assayed using a Bio-Rad ...

  20. Role of microRNA-195 in cardiomyocyte apoptosis induced by ...

    Indian Academy of Sciences (India)

    in 0.1% Triton X-100 and 0.1% natrium citricum for 15 min. Then the sections were washed in PBS thrice and incu- bated in 50 μL of TUNEL mix (Roche, .... phate polyacrylamide gel electrophoresis (SDS-PAGE) and blotted on to polyvinylidenedifluoride (PVDF) membranes, with average pore size of 0.22 μm. Then, the ...

  1. Contribution à l'étude de la salinisation de la nappe côtière de sahel ...

    African Journals Online (AJOL)

    La combinaison des outils, géologiques, hydrogéologiques, piézométriques et hydrochimiques a montré que les fortes salinités des eaux souterraines sont liées au phénomène d'intrusion marine (dans les premiers kilomètres de la côte), à celui du lessivage de la roche réservoir et de l'infiltration des eaux de retour.

  2. Routine screening of blood donations at Qingdao central blood bank, China, for hepatitis B virus (HBV) DNA with a real-time, multiplex nucleic acid test for HBV, hepatitis C virus, and human immunodeficiency virus Types 1 and 2. (United States)

    Yang, Zhongsi; Xu, Lei; Liu, Li; Feng, Qiuxia; Zhang, Longmu; Ma, Weijuan; Saldanha, John; Wang, Mingmin; Zhao, Lin


    The Roche cobas TaqScreen MPX test was used to evaluate the rate of hepatitis B surface antigen (HBsAg)-negative donations that were hepatitis B virus (HBV) DNA reactive from June 2010 to January 2011 in Qingdao, China. HBsAg-negative samples from 65,800 voluntary blood donors were tested with the cobas TaqScreen MPX test in pools of 6 on the Roche cobas s 201 blood screening platform. Samples positive for HBV DNA and negative for HBsAg were quantitated with the Roche COBAS AmpliPrep/COBAS TaqMan HBV test. In addition, serologic tests for HBsAg, hepatitis B surface antibody, anti-hepatitis B core antigen (anti-HBc), anti-hepatitis B e antigen (anti-HBe), and hepatitis B e antigen (HBe) were done using the Roche electrochemiluminescence immunoassay. A total of 80 nucleic acid amplification technology (NAT) test-reactive pools were identified and 59 pools (74%) resolved to a reactive sample. All samples were HBV DNA reactive and the viral load in each sample was quantitated. The viral loads of the samples ranged from less than 20 to 34,600 IU/mL; 13 samples (22%) had viral loads of more than 20 IU/mL, 27 samples (45.8%) had viral loads of less than 20 IU/mL, and 19 samples (32.2%) had undetectable viral loads. Of the 59 NAT-reactive samples, 40 (67.8%) were anti-HBc positive. Fifteen of the 59 samples could not be confirmed as NAT reactive either by an alternative NAT test or by serology. The HBV NAT yield in blood donors in Qingdao is 0.06% (38/65,800). This study confirmed the value of NAT for interdicting HBV-positive donations and preventing transfusion-transmitted HBV infections. © 2013 American Association of Blood Banks.

  3. 2301-IJBCS-Article-Mfonka Zakari

    African Journals Online (AJOL)


    savoir les roches granito-gneissiques et les basaltes transitionnels (Figures 3) de plateau d'âge précambrien (Moundi, 2004), sur lesquels sont identifiés quatre types de sols dont : les sols ferralitiques épais et majoritaires, les sols hydromorphes, les sols jeunes et les sols minéraux bruts (Njoya,. 2007). Le bassin versant ...

  4. Maoecrystal V: A formidable synthetic challenge

    Indian Academy of Sciences (India)

    Magdziak D, Meek S J and Pettus T R R 2004 Chem. Rev. 104 1383. 30. Roche S P and Porco Jr J A 2011 Angew. Chem. Int. Ed. 50 4068. 31. (a) Singh V 1999 Acc. Chem. Res. 32 324; (b) Singh V,. Praveena G D, Karki K and Mobin S M 2007 J. Org. Chem. 72 2058; (c) Singh V, Sahu P K, Sahu B C and. Mobin S M 2009 ...

  5. Multi-site study of HPV type-specific prevalence in women with cervical cancer, intraepithelial neoplasia and normal cytology, in England


    Howell-Jones, R; Bailey, A; Beddows, S; Sargent, A; de Silva, N; Wilson, G; Anton, J; Nichols, T; Soldan, K; Kitchener, H


    Background: Knowledge of the prevalence of type-specific human papillomavirus (HPV) infections is necessary to predict the expected, and to monitor the actual, impact of HPV immunisation and to design effective screening strategies for vaccinated populations. Methods: Residual specimens of cervical cytology (N=4719), CIN3/CGIN and cervical cancer biopsies (N=1515) were obtained from sites throughout England, anonymised and tested for HPV DNA using the Linear Array typing system (Roche). Resul...

  6. 435-IJBCS-Article-Prof E D Fiogbe

    African Journals Online (AJOL)


    industries et services publics. 397 000 m3 soit 217 tonnes de DBO5. Réalisé à partir des données de Soclo (1999) et Roche International (2000). Photos 3 & 4: Les latrines flottantes à côté du 2ème pont côté marché Dantokpa (Photo 3) et au quartier Agbato (Photo 4) (Badahoui, 21 février 2008). Les valeurs de température ...

  7. Cloning, purification and characterisation of a recombinant purine nucleoside phosphorylase from Bacillus halodurans Alk36

    CSIR Research Space (South Africa)

    Visser, Daniel F


    Full Text Available . 2008; Rocchietti et al. 2004). Enzymes provide regio- and stereoselectivity, and hence are an ideal option for nucleoside transglyco- sylation (Prasad et al. 1999; Utagawa 1999). The hydro- lysis reaction for the ribose decoupling reaction can... B. halodurans Alk36. Methods Materials All restriction enzymes and the T4-DNA ligase were pur- chased from Fermentas (Lithuania). The Roche high- fidelity PCR mix was used for all polymerase chain reac- tions. SDS-PAGE markers were purchased...

  8. European Science Notes Information Bulletin Reports on Current European/Middle Eastern Science, (United States)


    ionic strengths and are inter-tem eguatig vaiou celula fuctins.preted as equilibrium shifts between the two main con-tern regulating various cellular...Donnan poten- Sciences, University of Rome " La Sapienza", Italy, re- tial as well as diffusional potential generated by iono- ported on a thermodynamic...Hochuli, F. Hoffmann- La Roche & Co., Basel, lation of protein data (4) display of process flowsheets (5) Switzerland, presented an excellent review of

  9. Analytical characteristics and comparative evaluation of Aptima HIV-1 Quant Dx assay with Ampliprep/COBAS TaqMan HIV-1 test v2.0. (United States)

    Hatzakis, Angelos; Papachristou, Helen; Nair, Sangeetha J; Fortunko, Jacqueline; Foote, Tracy; Kim, HeeCheol; Peling, Tashi L; Worlock, Andrew J


    Quantitation of HIV-RNA is critically important for diagnosis, prognosis, treatment, monitoring and assessment of infectivity in HIV-1 infection. The objective of this study was to assess performance characteristics of the Aptima HIV-1 Quant Dx assay (Aptima), a new transcription mediated amplification (TMA), fully integrated and automated assay from Hologic Inc., San Diego, CA, USA. The analytical sensitivity, analytical specificity, precision and detection of HIV-1 subtypes were tested based on commercially available international standards or panels. A selected group of 244 anti-HIV-1 (+) plasma samples was used for comparison with Roche COBAS Ampliprep/COBAS TaqMan HIV- 1 test v2.0 (Roche CAP/CTM), (Roche Molecular Systems, Pleasanton, CA). The 50 and 95 % limit of detection were estimated at 4.9 (95 % CI 3.9-5.7) and 17.6 (15.2-21.2) IU/mL respectively. The specificity was found 99.83 (99.06-99.97) %. The standard deviations and coefficient of variations for panels with 50 and 100 copies/mL (1.7 and 2 log copies/mL) were 0.14 log copies/mL (8.67 %CV) and 0.18 log copies/mL (9.91 %CV) respectively. The detection rate for Aptima and Roche assays was 220/244 (90.2 %) and 217/244 (88.9 %) respectively. The Aptima assay is a sensitive, specific, precise and accurate test for measuring HIV-1 viral loads and for the detection of HIV-1 infections.

  10. Comparative approach to capture bacterial diversity in coastal waters

    DEFF Research Database (Denmark)

    Na, Hyunsoo; Kim, Ok-Sun; Yoon, Suk-hwan


    to investigate the bacterial community structure of coastal seawater collected from the Yellow Sea, Korea. For culture-independent studies, we used the latest model pyrosequencer, Roche/454 Genome Sequencer FLX Titanium. Pyrosequencing captured a total of 52 phyla including 27 candidate divisions from the water...... techniques available in microbial ecology. As different methods yielded different coverage, we suggest choosing the approach after carefully examining the scientific questions being asked....

  11. Un champ de cratères d'impact au sommet du Nkol Nyara à Leboudi ...

    African Journals Online (AJOL)

    L'importance des fontes représentées par une couche et des veinules de verre de couleur noire recouvrant ou affectant des gneiss clairs à grenat et disthène la roche trame hébergeant ces cratères, rend compte d'un processus thermique lié à l'arrivée au sommet du Nkol Nyara, d'un essaim ou d'une pluie de minuscules ...

  12. Introductory note on Emergent Unconscious Knowledge Networks (Asygnodic Networks

    Directory of Open Access Journals (Sweden)

    Henry Bakis


    Full Text Available This note introduces the following paper on the concept of Emergent Unconscious Knowledge Networks (Asygnodic Networks created by E. Roche and M. Blaine. The concept of asyngnosis explains a large number of diverse phenomena involving organizations, groups and decision making. It will present the genesis and definition of Asygnodic Networks and will focus on how they raise challenges to traditional theories of decision making and emerging social networks.

  13. The Economics of the Commodity Market Operations. (United States)


    public release; distribution is unlimited. SECURITY CLASSIFICATION OF THIS PAGE .z-.." REPORT DOCUMENTATION PAGE la REPORT SECURITY CLASSIFICATION lb...Randolph, Jr. Lieutenant, United States Navy B.S., La Roche College 1980 Submitted in partial fulfillment of the 4 requirements for the degree of MASTER OF...size and commercial importance, commodity trading began to take place in special markets known as " Bourses " in Europe and "Exchanges" in England. These

  14. Aerospace Materials Process Modelling (United States)


    Materials Researchr soc icl - STRASBOUtRG - June 1Qs(, 2 VERMOT DES ROCHES - Conf. " Transferts Tiiermiqiies ails Temp6ratuir’s 6 1 -es Fcole...consid6rer la carapaes comma des meidriaux composites at de la caractdriser an cons~quence au Prix de difficult63 exprimentales. L’Intdr~t de Is ddmarohe...COEFFICIENT DE TRANSFERT NOYAU - ONDUCTIVITC THERMIQUE - HALEUR MASS IQUE INTERFACES - COEFFICIENT DE TRANSFERI METAL-SOLE ET NOULE-SOLE EN SOLIDIFICATION

  15. Increasing Efficiency and Quality by Consolidation of Clinical Chemistry and Immunochemistry Systems with MODULAR ANALYTICS SWA


    Mocarelli, Paolo; Horowitz, Gary L.; Gerthoux, Pier Mario; Cecere, Rossana; Imdahl, Roland; Ruinemans-Koerts, Janneke; Luthe, Hilmar; Calatayud, Silvia Pesudo; Salve, Marie Luisa; Kunst, Albert; McGovern, Margaret; Ng, Katherine; Stockmann, Wolfgang


    MODULAR ANALYTICS Serum Work Area (in USA Integrated MODULAR ANALYTICS, MODULAR ANALYTICS is a trademark of a member of the Roche Group) represents a further approach to automation in the laboratory medicine. This instrument combines previously introduced modular systems for the clinical chemistry and immunochemistry laboratory and allows customised combinations for various laboratory workloads. Functionality, practicability, and workflow behaviour of MODULAR ANALYTICS Serum Work Area were ev...

  16. Nutritionally fastidious Ruminococct $ flovefociens : strains

    African Journals Online (AJOL)

    carbon and energy an acid hydrolysate of cellulose. The compositions of the remaining media will refer to the com- position of medium BN. This was based on the medium of Roch6 et al. (1973), with the following modifications: cellobiose,l0 g/l; methionine, 0,51 mM; acetic acid, 3 mM; propionic acid, I mM; isobutyric acid, 0,4 ...

  17. Pearl millet-based intercropping systems in the semiarid areas of ...

    African Journals Online (AJOL)

    Les traitements imposés sur l'unité expérimentale comprenaient des techniques de cultures appropriées et l'application des compostes et du phosphate des roches. Les zones écologiques étaient la région centrale du nord (RCN) et centrale du sud (RCS). La moyenne pluviométrique dans le RCN rangée entre 300 et 500 ...

  18. SHORT COMMUNICATION: Agronomic effectiveness of novel ...

    African Journals Online (AJOL)

    Une revue de la littérature montre que le travail sur engrais phosphatiques non conventionels a été fait exclusivement sur les roches (substances) phosphate sedimentaires. Le potentiel d'utilisation des nouveaux matériaux fertilisants phosphatiques dérivés de réactifs éruptifs Dorowa (Zimbabwe) ont été examiné dans une ...

  19. Observations sur le contact ouest du granite des Sept-Laux près du Rivier d’Allemont (Massif de Belledonne S.L.)

    NARCIS (Netherlands)

    Veen, van der A.H.


    La stratification des roches cristallines (d'âge antéstéphanien) des massifs centraux des Alpes est en général à peu près parallèle à la schistosité. Également les intrusions granitiques y sont plus ou moins concordantes. Cependant les recherches sous la direction du Professeur E. Niggli de Leiden

  20. Note sur un Lepidocyclina nouveau de Bornéo

    NARCIS (Netherlands)

    Schlumberger, C.


    Les travaux des géologues hollandais et de quelques savants anglais nous ont déjà fait connaître un bon nombre de foraminifères fossiles de Java et de Borneo, mais il reste encore à glaner après eux. Je viens de trouver dans une roche calcaire de Borneo que Mons. MARTIN, professeur à Leiden, a bien

  1. Note sur deux espèces de Lepidocyclina des Indes Néerlandaises

    NARCIS (Netherlands)

    Schlumberger, C.


    Je dois à l’obligeance de Monsieur K. MARTIN professeur de Géologie à l’Université de Leiden l’envoi pour ma collection de foraminifères de quelques doubles des roches rapportées de Java par Monsieur VERBEEK et j’ai eu la bonne fortune d’y rencontrer deux espèces d’Orbitoïdes du sous-genre


    African Journals Online (AJOL)

    SEI Joseph

    ces formations, la nature des phases minérales, les différentes phases de métamorphisme ayant affecté les corps minéralisés et les roches encaissantes, ainsi qu'une mise en évidence des différentes paragenèses entrant dans la composition des corps minéralisés [2,3]. Dans cette note, nous proposons la discussion de.

  3. Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1.


    Whetsell, A J; Drew, J B; Milman, G; Hoff, R; Dragon, E A; Adler, K; Hui, J; Otto, P; Gupta, P; Farzadegan, H


    Three nonradioisotopic polymerase chain reaction (PCR)-based detection techniques were evaluated for sensitivity and specificity in detecting human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells. The Roche prototype HIV-1 PCR assay, the Du Pont enzyme-linked oligonucleotide sandwich assay (ELOSA), and the Gen-Probe hybridization protection assay (HPA) were compared with a standard radioisotopic oligonucleotide solution hybridization (OSH) technique. A...

  4. Feasibility Analysis For Heating Tribal Buildings with Biomass

    Energy Technology Data Exchange (ETDEWEB)

    Steve Clairmont; Micky Bourdon; Tom Roche; Colene Frye


    This report provides a feasibility study for the heating of Tribal buildings using woody biomass. The study was conducted for the Confederated Salish and Kootenai Tribes of the Flathead Reservation in western Montana. S&K Holding Company and TP Roche Company completed the study and worked together to provide the final report. This project was funded by the DOE's Tribal Energy Program.

  5. Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns

    Czech Academy of Sciences Publication Activity Database

    Fíla, Jan; Čapková, Věra; Feciková, Jana; Honys, David


    Roč. 55, č. 3 (2011), s. 499-506 ISSN 0006-3134 R&D Projects: GA MŠk OC08011; GA ČR(CZ) GAP501/11/1462 Institutional research plan: CEZ:AV0Z50380511 Keywords : proteomics * Roche MagNA Lyser Instrument * ARABIDOPSIS-THALIANA Subject RIV: ED - Physiology Impact factor: 1.974, year: 2011

  6. 1056-IJBCS-Article-Oga Oms+

    African Journals Online (AJOL)


    H2O ont été utilisés pour l'étude de l'interaction eau- roche. Les teneurs isotopiques des eaux ont été déterminées par différentes méthodes telles que la spectrométrie de masse (18O, 2H, 13C), la spectrométrie de masse par accélérateur (14C-.

  7. Research Area 7.4: Identifying a Path Towards Rapid Discrimination of Infection Disease Outbreaks (United States)


    pyrosequencing technologies. The Ion Torrent PGM also uses emulsion PCR, but uses sequencing-by-synthesis and semiconductor technology to detect protons...specifically comparing the Roche 454 GS Junior, Life Technologies Ion Torrent PGM, and the Illumina MiSeq (Jünemann et al., 2013; Loman et al., 2012). In...microbial pathogens from the MiSeq, Ion Torrent , and the Pacific Biosciences RS instruments (Quail et al., 2012), reporting on coverage distribution, bias

  8. Effet Hall quantique, liquides de Luttinger et charges fractionnaires (United States)

    Roche, Patrice; Rodriguez, V.; Glattli, D. Christian

    We review some basic properties of the Fractional Quantum Hall Effect and particularly address the physics of the edge states. The chiral Luttinger liquid properties of the edges are discussed and probed experimentally using transport measurements. Shot noise measurements, which allow determination of the quasiparticle charge are also discussed. To cite this article: P. Roche et al., C. R. Physique 3 (2002) 717-732.

  9. Inflammation, Prostate Cancer and Negative Regulation of Androgen Receptor Expression (United States)


    inhibitor targeted to signaling proteins of innate immune responses. Proc. Natl. Acad. Sci. USA. Aug 15;103(33):12481-6 Appendices Original...example, HDAC1 plays a role in the NF-B-mediated transcriptional repression of the long-terminal repeat of the HIV genome, causing viral latency (20... seeded in 24-well plates, cultured overnight, and then transfected with plasmids for 48 h using Fugene6 liposome (Roche Molecular Biochemicals

  10. Contexte geologique de la mineralisation aurifere du prospect de ...

    African Journals Online (AJOL)

    Ces roches ont été affectées par une importante activité hydrothermale marquée par des filons et filonnets de quartz, calcite, ± tourmaline, ± sulfures. La minéralisation aurifère de Bobosso a été observée localement dans toutes les formations de la zone et ne présente donc pas de contrôle lithologique. Par contre, cette ...

  11. Walter Reed Army Institute of Research Annual Progress Report, Fiscal Year 1981. (United States)


    collection records were added to the com- puter data base: 1,939 pertain to Anopheles (Nyssorhynchus), 1,110 to Panama, 540 to Colombia and 61 to Ecuador...major areas. The first area is concerned with how the body affects the action of the candidate drug and includes studies to determine the pharma ...vitro PROBLEM: In recent years a combination of the drugs sulfadoxine and pyrimethamine (S-Py), marketed as Fansidar (Roche Laboratories) has been widely

  12. Seeing the Leaves and Not Missing the Forest: A Portuguese Perspective of the Solutrean


    Zilhão, João


    In a 1964 synthesis, Roche concluded that the Upper Palaeolithic of Portugal remained largely unknown, the presence of the Solutrean being the only fact that could be unambiguously ascertained. Subsequent work has radically changed this situation and, where the Solutrean is concerned, has established that its culture-stratigraphy sequence accords well with the traditional subdivision systematised for France by Smith, which was based on the successive appearance of different index fossils. Whe...

  13. Recent advances in the biogeochemistry of nitrogen in the ocean

    Digital Repository Service at National Institute of Oceanography (India)

    Naqvi, S.W.A.; Voss, M.; Montoya, J.P.

    in these zones becomes a very important ques- tion. In contrast, both anammox and heterotrophic denitrifi- cation have been found to be important in sediments (Tham- drup and Dalsgaard, 2002; Dalsgaard and Thamdrup, 2002; Trimmer et al., 2006), with the latter.... and Raymond, N.: Effects of oxygen on denitrification in marine sediments. Hydrobiologia, 207, 115–122, 1990. Breitbarth, E., Oschlies, A., and LaRoche, J.: Physiological con- straints on the global distribution of Trichodesmium – effect of temperature...

  14. RNA Binding Proteins Posttranscriptionally Regulate Genes Involved In Oncogenesis (United States)


    lysed in triple- detergent RIPA buffer with protease inhibitor cocktail (Roche, Pleasanton, CA). For nuclear and cytoplasmic fractionation, the NE-PER kit...Posttranscriptional regulation of IL-13 in T cells: role of the RNA-binding protein HuR. The Journal of allergy and clinical immunology 2008, 121(4):853-859...and western blot analysis. Western analysis was performed as described previously.12 For detection of VEGFα and TSP1 from tumors, triple- detergent

  15. Ėriuko skerdimo menas


    Uogintas, Arūnas


    The article discusses modern art campaigns and their representation in Lithuanian press. Strange and threatening headings of articles describing art campaigns are usually not threatening or cruel. Moreover, many of them remain unfulfilled. The article discusses a campaign arranged by a London artist B. De La Roche in Šiauliai Gallery of Art, which never took place and the artist’s promotion of the performance. Another example is E. Jans’s failure to organise the lamb slaughter performance at ...

  16. Realization of High-temperature Superconductivity in Nano-carbon Materials and Its Application (United States)


    in peer-reviewed journals, 1. T. Hashimoto , S. Kamikawa, J. Haruyama, D. Soriano, J. G. Pedersen, S. Roche “Tunneling magnetoresistance phenomena...Singapore 2014/10/21) ISBN-10: 9814303305 4. T. Hashimoto , S. Kamikawa, Y. Yagi, J. Haruyama, “Electronic Properties of Nanopore Edges of...narrow graphene nanoribbons”, Nanomaterials and Nanotechnology 4:12 | doi: 10.5772/58466 (2014) 6. T. Hashimoto , S. Kamikawa, Y. Yagi, J. Haruyama

  17. Mikroskopische Studien ueber Gesteine aus den Molukken. 2. Gesteine von Seran. Mit einem französischen Résumé

    NARCIS (Netherlands)

    Schroeder van der Kolk, J.L.C.


    Dans les pages précédentes j’ai décrit quelques uns des nombreux échantillons de roche recueillis par M. Martin pendant son voyage de Séran (Céram). Les types suivants en sont les plus intéressants, les granites à cordiérite, les diorites les péridotites, les andésites à augite, les gneiss, les

  18. Mikroskopische Studien ueber Gesteine aus den Molukken. 1. Gesteine von Ambon und den Uliassern. Mit einem französischen Résumé

    NARCIS (Netherlands)

    Schroeder van der Kolk, J.L.C.


    Dans le traité précédent j’ai décrit les types les plus caractéristiques parmi les échantillons de roche recueillis par M. MARTIN pendant ses voyages dans les Moluques. Ainsi il s’agit des Granites à biotite, des Péridotites, des Dacites, des Andésites à pyroxène, des Brèches volcaniques et aussi

  19. Usutu virus infections among blood donors, Austria, July and August 2017 - Raising awareness for diagnostic challenges. (United States)

    Bakonyi, Tamás; Jungbauer, Christof; Aberle, Stephan W; Kolodziejek, Jolanta; Dimmel, Katharina; Stiasny, Karin; Allerberger, Franz; Nowotny, Norbert


    Between July and August 2017, seven of 12,047 blood donations from eastern Austria, reacted positive to West Nile virus (WNV) in the cobas test (Roche). Follow-up investigations revealed Usutu virus (USUV) nucleic acid in six of these. Retrospective analyses of four blood donors diagnosed as WNV-infected in 2016 showed one USUV positive. Blood transfusion services and public health authorities in USUV-endemic areas should be aware of a possible increase of human USUV infections.

  20. Real-time PCR assays for hepatitis C virus (HCV) RNA quantitation are adequate for clinical management of patients with chronic HCV infection. (United States)

    Halfon, Philippe; Bourlière, Marc; Pénaranda, Guillaume; Khiri, Hacène; Ouzan, Denis


    Because of the use of viral kinetics during polyethylene glycol (PEG)-interferon-ribavirin therapy and the development of specific new anti-hepatitis C virus (anti-HCV) drugs, assessment of the efficacy of anti-HCV drugs needs to be based not on end-point PCR assays but on real-time PCR. The aim of this study was to determine if the two available commercial real-time PCR assays, the Abbott RealTime HCV assay and the Roche Cobas TaqMan HCV assay, can become the standard for HCV RNA quantification. We investigated the prognostic relevance of HCV RNA viral loads at baseline, week 4, and week 12 to a rapid and early virological response to antiviral therapy by using the two assays. Of 59 naïve patients chronically infected by HCV (41 infected with genotype 1) who were treated with ribavirin plus PEG-interferon alfa-2b for 48 weeks, 24 patients (41%) showed a sustained virological response (SVR). With the two assays, viral loads were highly correlated, irrespective of genotype (R2=0.94 for all cases). No difference in diagnostic value was found between the Abbott and Roche assays at week 4, with respective negative predictive values (NPVs) of 84% and 78% and positive predictive values (PPVs) of 62% and 56% (not significant), and at week 12, the respective NPVs were 91% and 90% and PPVs were 44% and 46% (not significant). At week 12, 83% (20/24) and 96% (23/24) of patients with SVR tested negative for HCV RNA by the Abbott and Roche assays, respectively (the difference is not significant). In conclusion, the high sensitivities and large dynamic ranges of the Abbott and Roche assays show that a single real-time quantitative PCR assay is fully adequate for clinical and therapeutic management of HCV.

  1. A systematic review and economic evaluation of bisphosphonates for the prevention of fragility fractures.


    Davis, S.; Martyn-St James, M.; Sanderson, J.; Stevens, J.; Goka, E.; Rawdin, A.; Sadler, S.; Wong, R.; Campbell, F.; Stevenson, M.; Strong, M.; Selby, P.; Gittoes, N.


    BACKGROUND: Fragility fractures are fractures that result from mechanical forces that would not ordinarily result in fracture. OBJECTIVES: To evaluate the clinical effectiveness and safety of bisphosphonates [alendronic acid (Fosamax(®) and Fosamax(®) Once Weekly, Merck Sharp & Dohme Ltd), risedronic acid (Actonel(®) and Actonel Once a Week(®), Warner Chilcott UK Ltd), ibandronic acid (Bonviva(®), Roche Products Ltd) and zoledronic acid (Aclasta(®), Novartis Pharmaceuticals UK Ltd)] for the p...

  2. Comparison of performance of classical clinical chemistry analysers with test-strip devices (Reflotron) and those based on film technology (Vitros) in external quality assessment (EQA) surveys. (United States)

    Wood, William Graham


    This article reports on the performance of two "dry" chemistry devices, (Reflotron, Roche Diagnostics and Vitros, Johnson & Johnson) and compared them with classical "wet" chemistry analysers in four commercially produced quality assessment samples (Roche PNU and PPU and Seronorm Human and Human High Controls) sent repeatedly over a 12-month observation period. Eleven analytes (including five enzymes) were studied, eight of which had target values set by reference method procedures. The results showed that both devices gave comparative results for the same sample sent in different EQA-surveys. Statistically significant differences which occurred were due to the high precision of measurement with a minimal shift in the measured concentrations. They had no clinical relevance in interpretation of results. Comparisons between "dry" and "wet" chemistry results for the same analyte were almost always statistically significantly different and often large enough to influence the clinical interpretation of results. Examples here were glucose and uric acid measured with the Reflotron and compared with other Roche devices (Cobas, Hitachi). The Vitros showed deviant values for urea and creatinine, when compared with other measuring devices using liquid reagents. Differences seen were constant over time, but must be seen in context with the matrices of the samples sent. The results show the long term stability of both reagents and test kits, a necessary prerequisite for long-term controlling of precision and indirectly accuracy of patient measurements.

  3. Mass-loss evolution of close-in exoplanets: Evaporation of hot Jupiters and the effect on population

    International Nuclear Information System (INIS)

    Kurokawa, H.; Nakamoto, T.


    During their evolution, short-period exoplanets may lose envelope mass through atmospheric escape owing to intense X-ray and extreme ultraviolet (XUV) radiation from their host stars. Roche-lobe overflow induced by orbital evolution or intense atmospheric escape can also contribute to mass loss. To study the effects of mass loss on inner planet populations, we calculate the evolution of hot Jupiters considering mass loss of their envelopes and thermal contraction. Mass loss is assumed to occur through XUV-driven atmospheric escape and the following Roche-lobe overflow. The runaway effect of mass loss results in a dichotomy of populations: hot Jupiters that retain their envelopes and super Earths whose envelopes are completely lost. Evolution primarily depends on the core masses of planets and only slightly on migration history. In hot Jupiters with small cores (≅ 10 Earth masses), runaway atmospheric escape followed by Roche-lobe overflow may create sub-Jupiter deserts, as observed in both mass and radius distributions of planetary populations. Comparing our results with formation scenarios and observed exoplanets populations, we propose that populations of closely orbiting exoplanets are formed by capturing planets at/inside the inner edges of protoplanetary disks and subsequent evaporation of sub-Jupiters.

  4. LUT observations of the mass-transferring binary AI Dra (United States)

    Liao, Wenping; Qian, Shengbang; Li, Linjia; Zhou, Xiao; Zhao, Ergang; Liu, Nianping


    Complete UV band light curve of the eclipsing binary AI Dra was observed with the Lunar-based Ultraviolet Telescope (LUT) in October 2014. It is very useful to adopt this continuous and uninterrupted light curve to determine physical and orbital parameters of the binary system. Photometric solutions of the spot model are obtained by using the W-D (Wilson and Devinney) method. It is confirmed that AI Dra is a semi-detached binary with secondary component filling its critical Roche lobe, which indicates that a mass transfer from the secondary component to the primary one should happen. Orbital period analysis based on all available eclipse times suggests a secular period increase and two cyclic variations. The secular period increase was interpreted by mass transfer from the secondary component to the primary one at a rate of 4.12 ×10^{-8}M_{⊙}/yr, which is in agreement with the photometric solutions. Two cyclic oscillations were due to light travel-time effect (LTTE) via the presence of two cool stellar companions in a near 2:1 mean-motion resonance. Both photometric solutions and orbital period analysis confirm that AI Dra is a mass-transferring binary, the massive primary is filling 69 % of its critical Roche lobe. After the primary evolves to fill the critical Roche lobe, the mass transfer will be reversed and the binary will evolve into a contact configuration.

  5. Pulmão profundo – Reacção celular ao VIH

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    Maria Alcide Tavares Marques


    sido instituída terapêutica anti-retroviral, primeiro grupo, enquanto o segundo grupo incluía doentes com doença mais prolongada, com vários episódios infecciosos oportunistas e tratamento anti-retroviral, para além de outros esquemas terapêuticos. Métodos: Todos os doentes após, consentimento informado, foram submetidos a exame broncoendoscó-pico e LBA, após anestesia local da orofaringe e cordas vocais com lidocaína a 2%, sob monitorização cárdio-respiratória. O LBA foi efectuado com base nas alterações evidenciadas pela telerradiografia do tórax: lobo médio nas lesões difusas, ou outro brônquio segmentar correspondente a alterações radiológicas localizadas A determinação das cargas virais no líquido de lavagem broncoalveolar (LLBA, em 9 doentes, foi efectuada pela quantificação do ARN-VIH1, através de PCR-RT, nas primeiras 4horas após a colheita (sendo cada amostra centrifugada, procedendose posteriomente nas aliquo-tas do sobrenadante à congelação a menos 80 º C, até à extracção e amplificação dos ácidos nucleicos. Realizou-se igualmente, na totalidade da amostra, a determinação das viremias plasmáticas (14 doentes, a partir de amostras de sangue colhido em EDTA, com processa-mento ulterior (separação do plasma e congelamento a menos 80 ºC. A extração e amplificação dos ácidos nucleicos foi efectuada por um método automatizado Cobas Ampliprep/Cobas Amplicor HIV1 Monitor TM Test, version 1.5 Roche Diagnostic Systems. Este método permite a obtenção duma sequência de 155 nucleótidos situada numa região altamente conservada do gene gag. Os resultados foram observados numa escala numérica com uma variação dinâmica situada entre as 50 e 750 000 cópias de ARN VIH1/cm3, procedendo-se posterior-mente à sua conversão logarítmica. No LLBA (10 doentes e no sangue

  6. Pulmão profundo – Reacção celular ao VIH

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    Maria Alcide Tavares Marques


    ída terapêutica anti-retroviral, primeiro grupo, enquanto o segundo grupo incluía doentes com doença mais prolongada, com vários episódios infecciosos oportunistas e tratamento antiretroviral, para além de outros esquemas terapêuticos.Métodos: Todos os doentes após, consentimento informado, foram submetidos a exame broncoendoscópico e LBA, após anestesia local da orofaringe e cordas vocais com lidocaína a 2%, sob monitorização cárdio--respiratória. O LBA foi efectuado com base nas alterações evidenciadas pela telerradiografia do tórax: lobo médio nas lesões difusas, ou outro brônquio segmentar correspondente a alterações radiológicas localizadas A determinação das cargas virais no líquido de lavagem broncoalveolar (LLBA, em 9 doentes, foi efectuada pela quantificação do ARN-VIH1, através de PCR-RT, nas primeiras 4horas após a colheita (sendo cada amostra centrifugada, procedendo-se posteriomente nas aliquotas do sobrenadante à congelação a menos 80° C, até à extracção e amplificação dos ácidos nucleicos. Realizou--se igualmente, na totalidade da amostra, a determinação das viremias plasmáticas (14 doentes, a partir de amostras de sangue colhido em EDTA, com processamento ulterior (separação do plasma e congelamento a menos 80°C. A extração e amplificação dos ácidos nucleicos foi efectuada por um método automatizado Cobas Ampliprep/Cobas Amplicor HIV1 Monitor TM Test, version 1.5 Roche Diagnostic Systems. Este método permite a obtenção duma sequência de 155 nucleótidos situada numa região altamente conservada do gene gag. Os resultados foram observados numa escala numérica com uma variação dinâmica situada entre as 50 e 750 000 cópias de ARN VIH1/cm3, procedendo-se posteriormente à sua conversão logarítmica. No LLBA (10 doentes e no sangue (13 doentes foi efectuado um

  7. Genotyping of hepatitis C virus-comparison of three assays. (United States)

    Haushofer, Alexander C; Berg, Jörg; Hauer, René; Trubert-Exinger, Doris; Stekel, Herbert G; Kessler, Harald H


    Genotyping of hepatitis C virus (HCV) is clinically relevant to epidemiology, prognosis, and therapeutical management of HCV infection. Accuracy and specificity of three assays for HCV genotyping/subtyping were determined. The TruGene HCV 5'NC Genotyping Kit (TruGene), which is a direct sequencing test and two assays based on reversed hybridization, Inno-LiPA HCV II assay and ViennaLab HCV Strip Assay, were compared. Amplification products generated by the Cobas Amplicor HCV Test were used. A total of 100 consecutive HCV RNA positive samples derived from patients with chronic hepatitis C were examined for their genotypes/subtypes by the three assays. Identification of genotypes and subtypes by the TruGene assay as reference test for the Inno-LiPA HCV II assay and the ViennaLab HCV Strip Assay or Inno-LiPA HCV II assay as reference test for the TruGene and the ViennaLab HCV Strip Assay showed similar results for overall accuracies (TruGene as reference test for Inno-LiPA HCV II and ViennaLab HCV Strip Assay, genotypes/subtypes: 100%/95.5% and 97%/92%; Inno-LiPA HCV II as reference test for TruGene and ViennaLab HCV Strip Assay, genotypes/subtypes: 99%/85.9% and 97%/87.9%) and specificities (TruGene as reference test for Inno-LiPA HCV II and ViennaLab HCV Strip Assay, genotypes/subtypes: 100%/97.8% and 99%/97.7%; Inno-LiPA HCV II as reference test for TruGene and ViennaLab HCV Strip Assay, genotypes/subtypes: 100%/99.4% and 99.7%/98%). The three assays were found to be reliable for the detection and discrimination of all HCV genotypes common in Europe and in North America and to be suitable for the routine diagnostic laboratory.

  8. Human papillomavirus prevalence among indigenous and non-indigenous Australian women prior to a national HPV vaccination program

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    Condon John R


    Full Text Available Abstract Background Indigenous women in Australia have a disproportionate burden of cervical cancer despite a national cervical screening program. Prior to introduction of a national human papilloma virus (HPV vaccination program, we determined HPV genotype prevalence by Indigenous status and residence in remote areas. Methods We recruited women aged 17 to 40 years presenting to community-based primary health services for routine Pap screening across Australia. A liquid-based cytology (LBC cervical specimen was tested for HPV DNA using the AMPLICOR HPV-DNA test and a PGMY09/11-based HPV consensus PCR; positive specimens were typed by reverse hybridization. We calculated age-adjusted prevalence by weighting to relevant population data, and determined predictors of HPV-DNA positivity by age, Indigenous status and area of residence using logistic regression. Results Of 2152 women (655 Indigenous, prevalence of the high-risk HPV genotypes was similar for Indigenous and non-Indigenous women (HPV 16 was 9.4% and 10.5%, respectively; HPV 18 was 4.1% and 3.8%, respectively, and did not differ by age group. In younger age groups, the prevalence of other genotypes also did not differ, but in those aged 31 to 40 years, HPV prevalence was higher for Indigenous women (35% versus 22.5%; P Conclusion Although we found no difference in the prevalence of HPV16/18 among Australian women by Indigenous status or, for Indigenous women, residence in remote regions, differences were found in the prevalence of risk factors and some other HPV genotypes. This reinforces the importance of cervical screening as a complement to vaccination for all women, and the value of baseline data on HPV genotype prevalence by Indigenous status and residence for the monitoring of vaccine impact.

  9. Therapy of chronic hepatitis C: Virologic response monitoring

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    Kuljić-Kapulica Nada


    Full Text Available Background/Aim. Virological testing is considered to be essential in the management of hepatitis C virus (HCV infection in order to diagnose infection, and, most importantly, as a quide for treatment decisions and assess the virological response to antiviral therapy. The aim of this study was to determine the rate of a sustained virological response (SVR and various factors associated with response rates in chronic hepatitis C infected patients treated with pegiinterferon alpha (PEGINF and ribavirin (RBV combination therapy. Methods. A total of 34 patients, treated with PEG-IFN and RBV were studied. Serum HCV-RNA was measured before the treatment, 12 weeks following the start of the therapy and 6 weeks after the treatment cessation. SVR was defined as undetectable serum HCV-RNA 6 months of post-treatment follow-up, virologic relapse (VR as relapse of HCV-RNA during the post-treatment follow-up. Serum HCV-RNA was measured with the Cobas Amplicor test. Results. At the end of post-treatment follow-up 19 (55.8% patients demonstrated a SVR. The majority of the patients were genotype 1 (27, and the other were genotype 3 (5 patients and genotype 4 (2 patients. There was VR in 6 patients 6 months after the therapy. In 9 patients HCV-RNA was positive after 12 weeks. Conclusion. We demonstrated that patients with chronic HCV infection can be successfully treated with combination of PEG-INF and RBV. This result emphasizes also that post-treatment follow-up to identify patients with SVR or VR could be important.

  10. Diagnostic accuracy of a prototype point-of-care test for ocular Chlamydia trachomatis under field conditions in The Gambia and Senegal.

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    Emma M Harding-Esch


    Full Text Available The clinical signs of active trachoma are often present in the absence of ocular Chlamydia trachomatis infection in low prevalence and mass treated settings. Treatment decisions are currently based on the prevalence of clinical signs, and this may result in the unnecessary distribution of mass antibiotic treatment. We aimed to evaluate the diagnostic accuracy of a prototype point-of-care (POC test, developed for field diagnosis of ocular C. trachomatis, in low prevalence settings of The Gambia and Senegal.Three studies were conducted, two in The Gambia and one in Senegal. Children under the age of 10 years were screened for the clinical signs of trachoma. Two ocular swabs were taken from the right eye. The first swab was tested by the POC test in the field and the result independently graded by two readers. The second swab was tested for the presence of C. trachomatis by Amplicor Polymerase Chain Reaction. In Senegal, measurements of humidity and temperature in the field were taken. A total of 3734 children were screened, 950 in the first and 1171 in the second Gambian study, and 1613 in Senegal. The sensitivity of the prototype POC test ranged between 33.3-67.9%, the specificity between 92.4-99.0%, the positive predictive value between 4.3-21.0%, and the negative predictive value between 98.0-99.8%. The rate of false-positives increased markedly at temperatures above 31.4°C and relative humidities below 11.4%.In its present format, this prototype POC test is not suitable for field diagnosis of ocular C. trachomatis as its specificity decreases in hot and dry conditions: the environment in which trachoma is predominantly found. In the absence of a suitable test for infection, trachoma diagnosis remains dependent on clinical signs. Under current WHO recommendations, this is likely resulting in the continued mass treatment of non-infected communities.

  11. Síndrome da meningite asséptica por enterovírus e Leptospira sp em crianças de Salvador, Bahia

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    Silva Hagamenon R.


    Full Text Available Objetivando verificar a freqüência de enterovírus (EV, leptospiras e arbovírus como agentes causais da síndrome da meningite asséptica (SMA, em períodos não-epidêmicos, e comparar os pacientes com e sem diagnóstico etiológico determinado, foram selecionados 112 pacientes de idade entre 3 meses e 15 anos, com suspeita clínica de SMA, referenciados para Hospital Couto Maia, especializado em Doenças Infecciosas e Parasitárias (Salvador, Bahia, Em 44,6% (n=50 a etiologia foi determinada: enterovírus em 37,7% (n=42 dos casos, pelo teste de PCR Amplicor, por cultura do líquor e/ou de fezes; a Leptospira sp. em 7,1% (n=8, pelo método da micro-aglutinação, e nenhum caso de arbovírus foi detectado (inibição da hemaglutinação passiva. Entre os 14 enterovírus dos 22 isolados, foram identificados seis diferentes sorotipos, sendo o Echovirus-4 predominante (27,2%; 6/22 entre outros (Coxsackie B2, B3, B6 e B9; EV 71. Conclui-se que, os enterovírus foram os agentes mais freqüentes, e que a leptospirose deve ser lembrada no diagnóstico diferencial da SMA. Uma vez que as características clínicas e liquóricas dos pacientes dos grupos com e sem determinação do agente etiológico foram semelhantes, pode-se supor que o diagnóstico presuntivo de SMA é de provável etiologia viral ou pela leptospira.

  12. Ekspresi Gen Family Bcl-2 dan Ekspresi Gen Protein Kanal Ion Vdac1 pada Oligozoospermia

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    Arni Amir


    Full Text Available AbstrakSalah satu bentuk infertilitas pada pria adalah jumlah spermatozoa yang kurang dari normal (Oligozoospermia. Berbagai mekanisme diduga berperan dalam terjadinya oligozoospermia, mulai dari faktor fisik, stress, makanan dan molekuler. Penelitian ini merupakan kajian molekuler terhadap pengaruh ekspresi gen Bax, Bcl-2 dan VDAC terhadap terjadinya oligozoospermia. Penelitian dilakukan terhadap 40 sampel oligozoospermia dan sebagai kontrol adalah golongan normozoospermia dengan jumlah yang sama banyak. Sperma diisolasi menggunakan Percoll hypaque. Isolasi RNA menggunakan High Pure RNA isolation kit (Roche. Selanjutnya dilakukan pembuatan cDNA menggunakan First Strand cDNA synthetis (Roche. Tingkat ekspresi gen dinilai menggunakan mesin Light Cycler 2.0 (Roche. Primer didesain menggunakan software primer 3 plus dan spesifikasi primer dianalisis dengan Basic Local Alignment Search Tool (Blast. Tingkat ekspresi dibandingkan menggunakan Housekeeping gene (β-actin. Berdasarkan data penelitian diketahui tingkat ekspresi VDAC dan BAX lebih tinggi pada kelompok oligozoospermia dibanding normo (p < 0.05, namun tidak ditemukan perbedaan pada gen Bcl-2 (p > 0.05. Berdasarkan proporsi ekspresi ditemukan sebagian besar kelompok oligozoospermia mengalami overekspresi pada gen VDAC dan BAX (90% dan 93.3%, namun tidak ada overkekspresi pada normo (p>0.05. Pada penelitian ini dapat disimpulkan bahwa overekspresi VDAC dan BAC berkaitan dengan kejadian oligozoospermiaKata kunci: Oligozoospermia, VDAC, Bax, Bcl-2, ekspresi genAbstractOne form of infertility in men is the number of spermatozoa which is less than normal (Oligozoospermia. Various mechanisms though have a role in the occurrence of oligozoospermia, it can be from physical factors, stress, food and molecular.This research is a molecular study of gen expression, Bax, Bcl2 and VDAC influence of the oligozoospermia’s occurrence.The research is conducted on 40 samples of oligozoospermia and as a

  13. Analytical and clinical performance of thyroglobulin autoantibody assays in thyroid cancer follow-up. (United States)

    Katrangi, Waddah; Grebe, Stephan K G; Algeciras-Schimnich, Alicia


    While thyroglobulin autoantibodies (TgAb) can result in false low serum thyroglobulin (Tg) immunoassay (IA) measurements, they might also be indicators of disease persistence/recurrence. Hence, accurate TgAb measurement, in addition to Tg quantification, is crucial for thyroid cancer monitoring. We compared the analytical and clinical performance of four commonly used TgAb IAs. We measured Tg by mass spectrometry (Tg-MS) and by four pairs of Tg and TgAb IAs (Beckman, Roche, Siemens, Thermo) in 576 samples. Limit of quantitation (LOQ) and manufacturers' upper reference interval cut-off (URI) were used for comparisons. Clinical performance was assessed by receiving operator characteristics (ROC) curve analysis. Quantitative and qualitative agreement between TgAb-IAs was moderate with R2 of 0.20-0.70 and κ from 0.41-0.66 using LOQ and 0.47-0.71 using URI. In samples with TgAb interference, detection rates of TgAb were similar using LOQ and URI for Beckman, Siemens, and Thermo, but much lower for the Roche TgAb-IA when the URI was used. In TgAb positive cases, the ROC areas under the curve (AUC) for the TgAb-IAs were 0.59 (Beckman), 0.62 (Siemens), 0.59 (Roche), and 0.59 (Thermo), similar to ROC AUCs achieved with Tg. Combining Tg and TgAb measurements improved the ROC AUCs compared to Tg or TgAb alone. TgAb-IAs show significant qualitative and quantitative differences. For 2 of the 4 TgAb-IAs, using the LOQ improves the detection of interfering TgAbs. All assays showed suboptimal clinical performance when used as surrogate markers of disease, with modest improvements when Tg and TgAb were combined.

  14. Survey of the Applications of NGS to Whole-Genome Sequencing and Expression Profiling

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    Jong-Sung Lim


    Full Text Available Recently, the technologies of DNA sequence variation and gene expression profiling have been used widely as approaches in the expertise of genome biology and genetics. The application to genome study has been particularly developed with the introduction of the next-generation DNA sequencer (NGS Roche/454 and Illumina/Solexa systems, along with bioinformation analysis technologies of whole-genome de novo assembly, expression profiling, DNA variation discovery, and genotyping. Both massive whole-genome shotgun paired-end sequencing and mate paired-end sequencing data are important steps for constructing de novo assembly of novel genome sequencing data. It is necessary to have DNA sequence information from a multiplatform NGS with at least 2× and 30× depth sequence of genome coverage using Roche/454 and Illumina/Solexa, respectively, for effective an way of de novo assembly. Massive short-length reading data from the Illumina/Solexa system is enough to discover DNA variation, resulting in reducing the cost of DNA sequencing. Whole-genome expression profile data are useful to approach genome system biology with quantification of expressed RNAs from a whole-genome transcriptome, depending on the tissue samples. The hybrid mRNA sequences from Rohce/454 and Illumina/Solexa are more powerful to find novel genes through de novo assembly in any whole-genome sequenced species. The 20× and 50× coverage of the estimated transcriptome sequences using Roche/454 and Illumina/Solexa, respectively, is effective to create novel expressed reference sequences. However, only an average 30× coverage of a transcriptome with short read sequences of Illumina/Solexa is enough to check expression quantification, compared to the reference expressed sequence tag sequence.

  15. Evaluation of the cobas® GT hepatitis C virus genotyping assay in G1-6 viruses including low viral loads and LiPA failures. (United States)

    Némoz, Benjamin; Roger, Léa; Leroy, Vincent; Poveda, Jean-Dominique; Morand, Patrice; Larrat, Sylvie


    Direct-acting antiviral (DAA) drug performances depend on the viral genotype. So international recommendations give typing of the virus a prerequisite for treatment choice and patient management. Commercially available HCV genotyping kits are scarce and this analysis is often in-house using tedious PCRs and Sanger sequencing, leading to a lack of standardization. A newly commercialized HCV genotyping assay based on real-time PCR has been developed by Roche Diagnostics (Mannheim, Germany). We compared this new assay with our in-house PCRs -sequencing technique on 101 regular samples and 81 LiPA failures or low viral load samples. No genotype or 1a/1b subtype mismatch was observed. Two samples were misidentified at the subtype level without clinical impact. Three genotype 1b and two genotype 1a samples with low viral load could not be subtyped. Nevertheless, 13 (13%) samples from the regular panel and 35 (43%) from the more difficult-to-type panels failed to give results on first pass with the Roche kit. Failures were mostly associated with genotype 3 subtype a, with genotype 4 subtype non-a, or with viral loads <200 IU/mL (p = 0.0061). The workflow allowed a non-specialized technician to obtain results in less than 4 hours whereas 2 to 3 days and experienced staff were required with the in-house assay. In conclusion, the Roche cobas® HCV GT kit is easy and rapid to use and provides reliable results. The high rate of uninterpretable results particularly for low viral load samples and less frequent genotypes, and the absence of subtyping for non-genotype 1 could require sending complex samples to a specialized laboratory.

  16. Comparison of three different assays for measuring thyroglobulin and thyroglobulin antibodies in patients with chronic lymphocytic thyroiditis. (United States)

    Cheng, Xinqi; Yu, Songlin; Jin, Cheng; Han, Song; Hu, Yingying; Zhang, Kui; Liu, Huaicheng; Qiu, Ling


    To evaluate how thyroglobulin (Tg) and thyroglobulin antibody (TgAb) results compare among different chemiluminescence methods. Serum specimens from 153 patients with thyroiditis and 127 apparently healthy individuals were collected at the Peking Union Medical College Hospital from September 2014 to December 2014. TgAb and Tg levels were analyzed by using three different platforms manufactured by Roche, Beckman, and Siemens. The χ 2 test was performed to analyze the TgAb results from the different methods. Passing-Bablok regression equations and Bland-Altman plots were used to estimate the relationship and bias among the Tg results obtained with the different analyses. The overall coincidence rates for the TgAb results were 82.9% between the Beckman and Roche methods, 78.6% between the Siemens and Roche methods, and 81.1% between the Siemens and Beckman methods. The borderline positive coincidence rate among the different TgAb methods was very poor (9.1-24.2%). Of the 280 samples, the TgAb results for 26.4% of the samples differed among the three CLIA platforms. A good linear relationship among the Tg levels was shown in TgAb-negative subjects analyzed with the different CLIA systems. However, for TgAb-positive subjects, the linear relationship of the Tg levels measured with the Siemens system, compared with the other two systems, was poor. Bland-Altman plots demonstrated significant differences among the different Tg assays. Current Tg and TgAb methods are highly variable and cannot be used interchangeably. Copyright © 2017. Published by Elsevier Inc.

  17. Comparison of the Reference Intervals Used for the Evaluation of Maternal Thyroid Function During Pregnancy Using Sequential and Nonsequential Methods (United States)

    Fan, Jian-Xia; Yang, Shuai; Qian, Wei; Shi, Feng-Tao; Huang, He-Feng


    Background: Maternal thyroid dysfunction is common during pregnancy, and physiological changes during pregnancy can lead to the overdiagnosis of hyperthyroidism and misdiagnosis of hypothyroidism with nongestation-specific reference intervals. Our aim was to compare sequential with nonsequential methods for the evaluation of thyroid function in pregnant women. Methods: We tested pregnant women who underwent their trimester prenatal screening at our hospital from February 2011 to September 2012 for serum thyroid stimulating hormone (TSH) and free thyroxine (FT4) using the Abbott and Roche kits. There were 447 and 200 patients enrolled in the nonsequential and sequential groups, respectively. The central 95% range between the 2.5th and the 97.5th percentiles was used as the reference interval for the thyroid function parameter. Results: The nonsequential group exhibited a significantly larger degree of dispersion in the TSH reference interval during the 2nd and 3rd trimesters as measured using both the Abbott and Roche kits (all P reference intervals were significantly larger in the nonsequential group than in the sequential group during the 3rd trimester as measured with both the Abbott (4.95 vs. 3.77 mU/L, P reference interval as measured with the Abbott kit during all trimesters (12.64 vs. 5.82 pmol/L; 7.96 vs. 4.77 pmol/L; 8.10 vs. 4.77 pmol/L, respectively, all P reference interval was only observed during the 2nd trimester with the Roche kit (7.76 vs. 5.52 pmol/L, P = 0.002). Conclusions: It was more reasonable to establish reference intervals for the evaluation of maternal thyroid function using the sequential method during each trimester of pregnancy. Moreover, the exclusion of pregnancy-related complications should be considered in the inclusion criteria for thyroid function tests. PMID:26996472

  18. Tidal stripping of stars near supermassive black holes

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    Blandford R.


    Full Text Available In a binary system composed of a supermassive black hole and a star orbiting the hole in an equatorial, circular orbit, the stellar orbit will shrink due to the action of gravitational radiation, until the star fills its Roche lobe outside the Innermost Stable Circular Orbit (ISCO of the hole or plunges into the hole. In the former case, gas will flow through the inner Lagrange point (L1 to the hole. If this tidal stripping process happens on a time scale faster than the thermal time scale but slower than the dynamical time scale, the entropy as a function of the interior mass is conserved. The star will evolve adiabatically, and, in most cases, will recede from the hole while filling its Roche lobe. We calculate how the stellar equilibrium properties change, which determines how the stellar orbital period and mass-transfer rate change through the “Roche evolution” for various types of stars in the relativistic regime. We envisage that the mass stream eventually hits the accretion disc, where it forms a hot spot orbiting the hole and may ultimately modulate the luminosity with the stellar orbital frequency. The ultimate goal is to probe the mass and spin of the hole and provide a test of general relativity in the strong-field regime from the resultant quasi-periodic signals. The observability of such a modulation is discussed along with a possible interpretation of an intermittent 1 hour period in the X-ray emission of RE J1034+ 396.

  19. Prevalence of hepatitis C in people who inject drugs in the cities of Rawalpindi and Islamabad, Pakistan. (United States)

    Waheed, Yasir; Najmi, Muzammil Hasan; Aziz, Hafsa; Waheed, Hasnain; Imran, Muhammad; Safi, Sher Zaman


    Pakistan has the second highest burden of hepatitis C (HCV) in the world. The major route of HCV transmission is contaminated blood or needle sharing. Seventy percent of people who inject drugs (PWIDs) shared needles at some time in their addiction history. The aim of the present study was to estimate the prevalence of HCV in PWIDs in cities of Pakistan. We enrolled 100 PWIDs from the Rawalpindi and Islamabad cities of Pakistan. Blood samples were taken in collection tubes and were subjected to HCV screening by using three rapid HCV screening kits including one step anti-HCV test, onsite HCV Ab rapid test and advance quality rapid anti-HCV test. All 100 blood samples were also subjected to HCV detection by using Elecsys anti-HCV II performed on the Roche Cobas 601 platform based on the ECLIA principle. Seventy-two percent of PWIDs showed the presence of HCV antibodies using the Roche anti-HCV II ECLIA test. We also compared the performance of different rapid kits in comparison with the anti-HCV II by Roche. The sensitivity of CTK kit was 84.72%, which was almost equal to the sensitivity by the SD Bioline HCV and Advanced Quality Rapid HCV tests, which was 83.33%. All three kits showed 100% specificity and positive predictive values. The results showed that the three market competitors of HCV rapid test showed almost equal results. The prevalence of HCV is very high in PWIDs in the capital twin cities of Pakistan. There is dire need to initiate the administration of a hepatitis test and treatment program for both high-risk and the general HCV-positive population. This is the optimal way to achieve HCV control targets established by the United Nations Sustainable Development Goals and Global Health Sector Strategy by WHO.


    Energy Technology Data Exchange (ETDEWEB)

    Hyodo, Ryuki; Charnoz, Sébastien [Institut de Physique du Globe, F-75005 Paris, France (France); Genda, Hidenori [Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo 152-8550 (Japan); Ohtsuki, Keiji [Department of Planetology, Kobe University, Kobe 657-8501 (Japan)


    Centaurs are minor planets orbiting between Jupiter and Neptune that have or had crossing orbits with one or more giant planets. Recent observations and reinterpretation of previous observations have revealed the existence of ring systems around 10199 Chariklo and 2060 Chiron. However, the origin of the ring systems around such a minor planet is still an open question. Here, we propose that the tidal disruption of a differentiated object that experiences a close encounter with a giant planet could naturally form diverse ring–satellite systems around the Centaurs. During the close encounter, the icy mantle of the passing object is preferentially ripped off by the planet's tidal force and the debris is distributed mostly within the Roche limit of the largest remnant body. Assuming the existence of a 20−50 wt% silicate core below the icy mantle, a disk of particles is formed when the objects pass within 0.4–0.8 of the planet's Roche limit with the relative velocity at infinity 3−6 km s{sup −1} and 8 hr initial spin period of the body. The resultant ring mass is 0.1%–10% of the central object's mass. Such particle disks are expected to spread radially, and materials spreading beyond the Roche limit would accrete into satellites. Our numerical results suggest that ring formation would be a natural outcome of such extreme close encounters, and Centaurs can naturally have such ring systems because they cross the orbits of the giant planets.

  1. Evaluating new HbA1c methods for adoption by the IFCC and NGSP reference networks using international quality targets. (United States)

    Lenters-Westra, Erna; English, Emma


    As a reference laboratory for HbA1c, it is essential to have accurate and precise HbA1c methods covering a range of measurement principles. We report an evaluation of the Abbott Enzymatic (Architect c4000), Roche Gen.3 HbA1c (Cobas c513) and Tosoh G11 using different quality targets. The effect of hemoglobin variants, other potential interferences and the performance in comparison to both the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) and the National Glycohemoglobin Standardization Program (NGSP) reference systems was assessed using certified evaluation protocols. Each of the evaluated HbA1c methods had CVs <3% in SI units and <2% in NGSP units at 46 mmol/mol (6.4%) and 72 mmol/mol (8.7%) and passed the NGSP criteria when compared with six secondary reference measurement procedures (SRMPs). Sigma was 8.6 for Abbott Enzymatic, 3.3 for Roche Cobas c513 and 6.9 for Tosoh G11. No clinically significant interference was detected for the common Hb variants for the three methods. All three methods performed well and are suitable for clinical application in the analysis of HbA1c. Partly based on the result of this study, the Abbott Enzymatic method on the Architect c4000 and the Roche Gen.3 HbA1c on the Cobas c513 are now official, certified IFCC and NGSP SRMPs in the IFCC and NGSP networks. Sigma metrics quality criteria presented in a graph distinguish between good and excellent performance.

  2. Real-Time PCR Assays for the Quantification of HCV RNA: Concordance, Discrepancies and Implications for Response Guided Therapy. (United States)

    Strassl, Robert; Rutter, Karoline; Stättermayer, Albert Friedrich; Beinhardt, Sandra; Kammer, Michael; Hofer, Harald; Ferenci, Peter; Popow-Kraupp, Theresia


    Monitoring of chronic Hepatitis C (CHC) treatment relies on HCV RNA quantification by means of real-time PCR methods. Assay specific analytical sensitivities may impact therapy management. Comparative analysis between three commercial assays (Roche COBAS AmpliPrep/COBAS TaqMan Version 1 (CAP/CTM Ver. 1), Version 2 (CAP/CTM Ver. 2) and the Abbott RealTime HCV (ART) assay) was performed on 247 available samples taken at key decision time points during antiviral therapy of 105 genotype 1 patients (triple therapy: n = 70; dual therapy: n = 35). Overall concordance of HCV RNA measurements was high between the two Roche systems (89%; n = 220/247) but lower between the Roche assays and the ART (CAP/CTM Ver. 1 vs ART: 77.3%; n = 191/247 and CAP/CTM v.2 vs ART: 80.1%; n = 198/247). Most discrepancies were noted in week 4/8 samples with residual viremia (HCV RNA by CAP/CTM Ver. 1 (

  3. Real-Time PCR Assays for the Quantification of HCV RNA: Concordance, Discrepancies and Implications for Response Guided Therapy.

    Directory of Open Access Journals (Sweden)

    Robert Strassl

    Full Text Available Monitoring of chronic Hepatitis C (CHC treatment relies on HCV RNA quantification by means of real-time PCR methods. Assay specific analytical sensitivities may impact therapy management.Comparative analysis between three commercial assays (Roche COBAS AmpliPrep/COBAS TaqMan Version 1 (CAP/CTM Ver. 1, Version 2 (CAP/CTM Ver. 2 and the Abbott RealTime HCV (ART assay was performed on 247 available samples taken at key decision time points during antiviral therapy of 105 genotype 1 patients (triple therapy: n = 70; dual therapy: n = 35.Overall concordance of HCV RNA measurements was high between the two Roche systems (89%; n = 220/247 but lower between the Roche assays and the ART (CAP/CTM Ver. 1 vs ART: 77.3%; n = 191/247 and CAP/CTM v.2 vs ART: 80.1%; n = 198/247. Most discrepancies were noted in week 4/8 samples with residual viremia (

  4. Diversidade e estrutura da comunidade bacteriana associada às armadilhas da planta carnívora Utricularia gibba (Lentibulariaceae) e ao ambiente aquático.


    Almir José Ferreira


    A diversidade microbiana em ambientes aquáticos e sua associação com plantas carnívoras ainda é pouco estudada. Assim, a comunidade bacteriana da planta carnívora Utricularia gibba e do seu meio aquático foi avaliada por meio do seqüenciamento em larga escala (454 Roche) de uma biblioteca do gene 16S rRNA. Os resultados indicaram que a comunidade bacteriana na água é significativamente diferente da comunidade dos utrículos. Além disso, a comunidade bacteriana da água é composta principalmente...

  5. Sistema de parques: origen y evolución de un principio ‘estructurador’ de lo urbano


    Jiménez Jiménez, Marina


    Resumen de la tesis doctoral «Sistema de Parques: origen y evolución de un principio ‘estructurador’ de lo urbano», defendida por Dña. Marina Jiménez Jiménez en el Instituto Universitario de Urbanística de la Universidad de Valladolid el 20 de marzo de 2009. Director: Dr. Juan Luis de las Rivas Sanz (Universidad de Valladolid). Tribunal: Dr. Alfonso Álvarez Mora, Dr. Luis Santos y Ganges, Dr. José Javier Maderuelo Raso, Dr. Fernando Roch Peña y Dr. Estanislao de Luis Calabuig.

  6. Collected papers of V K Patodi

    CERN Document Server

    Atiyah, Michael Francis


    Vijay Kumar Patodi was a brilliant Indian mathematicians who made, during his short life, fundamental contributions to the analytic proof of the index theorem and to the study of differential geometric invariants of manifolds. This set of collected papers edited by Prof M Atiyah and Prof Narasimhan includes his path-breaking papers on the McKean-Singer conjecture and the analytic proof of Riemann-Roch-Hirzebruch theorem for Kähler manifolds. It also contains his celebrated joint papers on the index theorem and the Atiyah-Patodi-Singer invariant.

  7. Preventing Prostate Cancer Metastasis by Targeting Exosome Secretion (United States)


    successfully established the prostate cancer and bone marrow cells lines in the lab; iii ) successfully extracted exosomes from the media, using a variety of...antagonistic or synergistic arrangements of: (i) RBPs and miRNAs; (ii) RBPs and RBPs and ( iii ) miRNAs on cytoplasmic mRNAs and their impact in cell...buffer (50 mM HEPES pH 7.5, 150 mM KCl, 2 mM EDTA, 1% NP40, one Complete mini protease inhibitor tablet and one PhosStop tablet per 10 mL (Roche, Basel

  8. Violencia de los usuarios hacia el personal de enfermería : evaluación del riesgo laboral y análisis de las consecuencias psicológicas


    Galián Muñoz, Inmaculada


    La presente tesis doctoral se centra en el estudio de la exposición a la violencia de los usuarios del personal de enfermería de los hospitales públicos de la Región de Murcia, ya que el riesgo de violencia laboral presenta una especial importancia en el ámbito sanitario, siendo el colectivo de enfermería uno de los más afectados tal y como se refleja en distintos estudios (Franz, Zeh, Schablon, Kuhnert y Nienhaus, 2010; Kling, Yassi, Smailes, Lovato y Koehoorn, 2009; Roche, Diers, Duffield y...

  9. Early Biochemical Screening for Fetal Aneuploidy in the First Trimester

    DEFF Research Database (Denmark)

    Tørring, Niels


    8+0 to 14+0 weeks, and clinical test performance of risk assesment was conducted. Results The in-between day imprecision of the Elecsys® free βhCG and PAPP-A assays were between 1.0 and 2.8%. Comparison (Passing/Bablok regression) of free βhCG and PAPP-A from Roche Elecsys® and the Brahms Kryptor...... with the standards for biochemical assays for prenatal screening set by the Fetal Medicine Foundation, with low assay imprecision, and a high clinical performance of prenatal screening for fetal trisomy in the first trimester....

  10. Effect of estimated glomerular filtration rate on plasma concentrations of B-type natriuretic peptides measured with multiple immunoassays in elderly individuals

    DEFF Research Database (Denmark)

    Schou, M.; Alehagen, U.; Goetze, J.P.


    linear regression models. RESULTS: In univariate analyses the effect of a 10% decrease in eGFR on proBNP concentrations was a 15% (95% confidence interval 11% to 18%), 9% (5% to 13%) and 21% (14% to 28%) increase. In multivariate models the effect was a 7% (3% to 11%), 4% (2% to 6%) and 13% (4% to 20....... SETTING: 474 elderly outpatients with suspected heart failure (prevalence 13%) from the primary care. MAIN OUTCOME MEASURES: The effects of eGFR on proBNP, measured with three different immunoassays (Roche Diagnostics, Oslo and Copenhagen), and BNP (Shionogi) concentrations were evaluated by multiple...

  11. American Medical Association sponsors press conference in home care. (United States)

    Bayne, C G


    On September 12, 1996, the American Medical Association, with an educational grant from Hoffmann-La Roche, sponsored a National Press Conference in New York City at the Millenium Broadway Hotel on Times Square. Attended by more than 40 of the nation's top health care correspondents from the leading magazine and newspapers in the country, this conference was designed to promote "The Revolution in Home and Outpatient Care." With an emphasis on new sites and new technologies, speakers from the Academy of Homecare Physicians presented a number of related subjects.

  12. Comparison of two automated quantitative immunoassays for the determination of C reactive protein concentrations. (United States)

    Barclay, E A; Coia, J E; Kale, P C; Masterton, R G


    Two quantitative, automated methods for the determination of C reactive protein (CRP) were compared: turbidimetry (Cobas Fara II, Roche, Welwyn Garden City, UK) and fluorescence polarisation TDx, Abbott, Wokingham, UK). One hundred and twenty routine serum samples submitted for measurement of CRP were tested using both procedures. The results were compared using regression line analysis and showed a high degree of correlation (r2 = 0.99, X coefficient = 1.01, constant = 0.11). C reactive protein can be accurately measured using the automated turbidimetric method which can be recommended as an alternative to fluorescence polarisation. Images PMID:7876389

  13. Development and characterization of ten polymorphic microsatellite loci for the Great Capricorn beetle (Cerambyx cerdo) (Coleoptera: Cerambycidae)

    Czech Academy of Sciences Publication Activity Database

    Drag, Lukáš; Kosnar, J.; Čížek, Lukáš


    Roč. 5, č. 4 (2013), s. 907-909 ISSN 1877-7252 R&D Projects: GA TA ČR TA02021501 Grant - others:GA JU(CZ) 168/2013/P; project Biodiversity of forest ecosystem(CZ) CZ.1.07/2.3.00/20.0064; MŠMT ČR(CZ) 1904/2012 Institutional support: RVO:60077344 Keywords : xylophagous insect * Roche 454 * SSR markers Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.136, year: 2013

  14. Molecular detection of Coxiella burnetii in goat bulk milk samples in ...

    African Journals Online (AJOL)



    Dec 14, 2011 ... volume of 25 µl containing 5 µl of DNA sample, 0.5 mM MgCl2, 0.2. mM (each) dNTPs, 1 µM primer OMP1, 1 µM primer OMP2 and 0.5. U/reaction of Taq DNA polymerase (Roche Applied Science,. Germany). The PCR assay was performed at 94°C for 4 min and then for 30 cycles of 94°C for 1 min, 56°C for ...

  15. Active and realistic passive marijuana exposure tested by three immunoassays and GC/MS in urine

    Energy Technology Data Exchange (ETDEWEB)

    Mule, S.J.; Lomax, P.; Gross, S.J.


    Human urine samples obtained before and after active and passive exposure to marijuana were analyzed by immune kits (Roche, Amersham, and Syva) and gas chromatography/mass spectrometry (GC/MS). Seven of eight subjects were positive for the entire five-day test period with one immune kit. The latter correlated with GC/MS in 98% of the samples. Passive inhalation experiments under conditions likely to reflect realistic exposure resulted consistently in less than 10 ng/mL of cannabinoids. The 10-100-ng/mL cannabinoid concentration range essential for detection of occasional and moderate marijuana users is thus unaffected by realistic passive inhalation.

  16. Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing

    DEFF Research Database (Denmark)

    Bohmann, Kristine; Monadjem, Ara; Noer, Christina Lehmkuhl


    Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep......-sequence uniquely 5′ tagged insect-generic barcode cytochrome c oxidase I (COI) fragments, that were PCR amplified from faecal pellets of two free-tailed bat species Chaerephon pumilus and Mops condylurus (family: Molossidae). Although the analyses were challenged by the paucity of southern African insect COI...

  17. Effects of aniracetam on delayed matching-to-sample performance of monkeys and pigeons. (United States)

    Pontecorvo, M J; Evans, H L


    A 3-choice, variable-delay, matching-to-sample procedure was used to evaluate drugs in both pigeons and monkeys while tested under nearly-identical conditions. Aniracetam (Roche 13-5057) improved accuracy of matching at all retention intervals following oral administration (12.5, 25 and 50 mg/kg) to macaque monkeys, with a maximal effect at 25 mg/kg. Aniracetam also antagonized scopolamine-induced impairment of the monkey's performance. Intramuscular administration of these same doses of aniracetam produced a similar, but not significant trend toward improved matching accuracy in pigeons.

  18. Dynamics of the last glaciation in eastern Svalbard as inferred from glacier-movement indicators


    Salvigsen, Otto; Adrielsson, Lena; Hjort, Christian; Kelly, Michael; Landvik, Jon Y.; Ronnert, Lars


    Glacial striae and other ice movement indicators such as roche moutonées, glacial erratics, till fabric and glaciotectonic deformation have been used to reconstruct the Late Weichselian ice movements in the region of eastern Svalbard and the northern Barents Sea. The ice movement pattern may be divided into three main phases: (1) a maximum phase when ice flowed out of a centre east or southeast of Kong Karls Land. At this time the southern part of Spitsbergen was overrun by glacial ice from t...

  19. Characterisation Of The Porcine Lung Transcriptome Using High-Throughput Pyrosequencing

    DEFF Research Database (Denmark)

    Panitz, Frank; Nielsen, Rasmus Ory; Andersen, Pernille K

    abundance. Our objective was to investigate animals previously not affected by lung disease and those that had been affected. To this end lung tissue samples were collected, separately pooled and tagged before sequencing using the Roche/454 FLX platform. We sequenced about one million reads that were...... clustered and mapped to the current pig genome reference sequence. Identified genes or clusters were annotated for functional classes and mined for singe nucleotide polymorphisms. In addition, we compared gene expression between sample groups in order to investigate possible changes in the lung...

  20. [Epidermic chalone receptors in psoriasis treated with retinoid:Ro 10-9359]. (United States)

    Vignale, R A; Lasalvia, E; Espasandín, J; Borras, A


    The epidermic chalone receptor of skin with lesion and without lesion are studied on 20 patients with active psoriasis. The testlest were performed before and two month after oral treatment with RO 10-9359. Roche. The results show significant variation with an increased a the number of receptors after treatment. As these receptors are in the cell membrane, the authors consider that RO 10-9359 action in on the membrane and its surrounding, making in possible to the epithelial cell to begin its autoregulation, autocontrol and differentiation when the specific activity of the chalones become normal.

  1. The Journal of the Institute of Chemistry of Ireland


    Hobbs, Patrick; Corish, John; Kilcawley, Kieran; Phelan, James; Roche, James J.; Poc, Pavel; Rea, Michael; Maguire, Denise


    A Message from the President. p6 -- Editorial p8 -- Congress: Food Chemistry p11 -- Inorganic Ireland 2017 p18 -- 69th Irish University Chemistry Research Colloquium 2017 p27 -- Irish Lab Awards 2017 Winners p31 -- The New Elements. Prod J Corish p36 -- TCD: New Head of Chemistry School p43 -- Review; Food & beverages Flavours. Dr Kieran Kilcawley p45 -- UPLC: Validation for Assay. J Phelan, Dr J Roche p50 -- Glyphosate a European perspective. EuCheMS p65 -- Young Chemist Prize, RIA p72 -- IC...

  2. [Cutaneous melanoma - "black death" of modern times? Traces in contemporary literature]. (United States)

    Bahmer, F A; Bahmer, J A


    Cutaneous melanoma, sometimes labeled as "black skin cancer", is increasing in frequency and becoming a more common literary motive. In US literature, Sylvia Plath and Charles Bukowski depicted melanoma more than 50 years ago, later Stephen King and Thomas C. Boyle. In German literature, Charlotte Roche shortly mentioned this tumor. Jörg Pönnighaus, both poet and dermatologist, intensively deals in his poems with the effects melanoma has on patients and doctors alike. Melanoma definitely is not the "Black Death" of modern times. However, the perception of this tumor as extremely malignant and as life-threatening makes melanoma a metaphor of the deadly danger of cancer.

  3. Advances in astronomy and astrophysics 9

    CERN Document Server

    Kopal, Zdenek


    Advances in Astronomy and Astrophysics, Volume 9 covers reviews on the advances in astronomy and astrophysics. The book presents reviews on the Roche model and its applications to close binary systems. The text then describes the part played by lunar eclipses in the evolution of astronomy; the classical theory of lunar eclipses; deviations from geometrical theory; and the methods of photometric observations of eclipses. The problems of other phenomena related in one way or another to lunar eclipses are also considered. The book further tackles the infrared observation on the eclipsed moon, as

  4. Specificity of the Linear Array HPV Genotyping Test for detecting human papillomavirus genotype 52 (HPV-52)


    Kocjan, Boštjan; Poljak, Mario; Oštrbenk, Anja


    Introduction: HPV-52 is one of the most frequent human papillomavirus (HPV) genotypes causing significant cervical pathology. The most widely used HPV genotyping assay, the Roche Linear Array HPV Genotyping Test (Linear Array), is unable to identify HPV- 52 status in samples containing HPV-33, HPV-35, and/or HPV-58. Methods: Linear Array HPV-52 analytical specificity was established by testing 100 specimens reactive with the Linear Array HPV- 33/35/52/58 cross-reactive probe, but not with the...

  5. Private visit to the CMS assembly site of Dr. Fidel Castro Diaz-Balart from the Superior Institute of Sciences and Nuclear Technologies, Havana, accompanied by His Excellency Mr. Emilio Caballero, Ambassador, Permanent Mission of Cuba in Paris.

    CERN Multimedia

    Maximilien Brice


    Photo 01: Left to right: His Excellency Mr Emilio Caballero; Prof. Tejinder Virdee, Deputy Spokesman of the CMS experiment; Dr Fidel Castro Diaz-Balart; Dr Matthias Schroeder, physicist, Experimental Physics division; Mrs Noëlle Levy, Casa del Habano, Geneva; Dr John Ellis, Adviser for Non-Member State relations; Dr Christian Roche, Senior Advisor to the Director-General. Photo 02: Left to right: His Excellency Mr Emilio Caballero, Prof. Tejinder Virdee, Dr Fidel Castro Diaz-Balart; Dr Matthias Schroeder; Mrs Noëlle Levy, Prof. Juan Antonio Rubio, Head of the Education and Technology Transfer division; Dr John Ellis.

  6. Active and realistic passive marijuana exposure tested by three immunoassays and GC/MS in urine

    International Nuclear Information System (INIS)

    Mule, S.J.; Lomax, P.; Gross, S.J.


    Human urine samples obtained before and after active and passive exposure to marijuana were analyzed by immune kits (Roche, Amersham, and Syva) and gas chromatography/mass spectrometry (GC/MS). Seven of eight subjects were positive for the entire five-day test period with one immune kit. The latter correlated with GC/MS in 98% of the samples. Passive inhalation experiments under conditions likely to reflect realistic exposure resulted consistently in less than 10 ng/mL of cannabinoids. The 10-100-ng/mL cannabinoid concentration range essential for detection of occasional and moderate marijuana users is thus unaffected by realistic passive inhalation

  7. Gut microbial markers are associated with diabetes onset, regulatory imbalance, and IFN-γ level in NOD mice

    DEFF Research Database (Denmark)

    Krych, Lukasz; Nielsen, Dennis Sandris; Hansen, Axel Kornerup


    Gut microbiota regulated imbalances in the host's immune profile seem to be an important factor in the etiology of type 1 diabetes (T1D), and identifying bacterial markers for T1D may therefore be useful in diagnosis and prevention of T1D. The aim of the present study was to investigate the link...... between the early gut microbiota and immune parameters of non-obese diabetic (NOD) mice in order to select alleged bacterial markers of T1D. Gut microbial composition in feces was analyzed with 454/FLX Titanium (Roche) pyro-sequencing and correlated with diabetes onset age and immune cell populations...

  8. L'atténuation intrinsèque des ondes sismiques. Troisième partie : Mécanismes régissant l'atténuation. Intrinsic Attenuation of Seismic Waves. Part Three: Mechanisms Governing Attenuation

    Directory of Open Access Journals (Sweden)

    Bourbie T.


    Full Text Available Les mécanismes qui régissent l'atténuation sont très complexes et encore mal compris actuellement. Certains mécanismes ont pu être éliminés comme le phénomène de frottement grain à grain du fait des amplitudes de déformation usuelles en sismique. Il. semble que les mécanismes responsables soient différents dans le cas de roches sèches (aux conditions de laboratoire et dans le cas de roches plus ou moins saturées. Dans le cas de roches sèchesl'atténuation semble provenir à la fois d'un hystérésis microcapillaire et d'une rupture des liaisons hydrogènes entre les hydroxyles de la surface et les molécules d'eau. Dans le cas de roches saturées, les candidats les plus probables au phénomène d'atténuation sont les mécanismes de Biot et de squirt flow . Le mécanisme de Biot est la possibilité d'avoir des mouvements différentiels globaux (ou plutôt moyennés à l'échelle de l'échantillon entre la matière solide et le fluide. Le squirt flowconsiste en un phénomène de relaxation visqueuse dont l'excitateur est extérieur : le liquide à l'intérieur de la cavité poreuse jaillit sous l'effet d'une pression de pore locale. The mechanisms governing attenuation are highly complex and still poorly understood at present. Some mechanisms have been eliminated, such as the grain-to-grain friction, due to the usual deformation amplitudes in seismic exploration. The mechanisms responsible for attenuation seem to be different for dry rocks (under laboratory conditions and more or less saturated rocks. For dryrocks, attenuation seems to come from both a microcapillary hysteresis and a break of the hydrogen bonds between the surface hydroxyls and water molecules. For saturated rocks, the most probable causes of attenuation are Biot mechanisms and squirt flow. The Biot mechanism Is the possibility of having overall differential movements (or rather averaged ones on the scale of the sample between the solid matrix and the fluid

  9. Influence des caracteristiques petro-structurales sur les proprietes ...

    African Journals Online (AJOL)

    Deux variétés de quartzites de l'unité litho-tectonique de l'Atacora dans la chaîne panafricaine des Dahoméyides au Nord-Ouest du Bénin ont fait l'objet d'études pétrostructurales et mécaniques en vue de la détermination de l'influence de la minéralogie et de la déformation des roches sur leurs propriétés géotechniques.

  10. La géologie du Massif du Saint-Barthélémy (Pyrénées, France)

    NARCIS (Netherlands)

    Zwart, H.J.


    Comme la carte de la page 8 le montre le Massif du Saint-Barthélemy se trouve dans le Dépt. de l'Ariège à l'Est et au Nord de la rivière dont le département porte le nom et entre Foix et Ax-les-Thermes. Le terrain examiné est formé entièrement de roches paléozoïques ou plus anciennes, bordées au

  11. Interacting binary stars

    CERN Document Server

    Sahade, Jorge; Ter Haar, D


    Interacting Binary Stars deals with the development, ideas, and problems in the study of interacting binary stars. The book consolidates the information that is scattered over many publications and papers and gives an account of important discoveries with relevant historical background. Chapters are devoted to the presentation and discussion of the different facets of the field, such as historical account of the development in the field of study of binary stars; the Roche equipotential surfaces; methods and techniques in space astronomy; and enumeration of binary star systems that are studied

  12. [Tests for early diagnosis of pregnancy]. (United States)

    Garcia Brandao, L


    Kits for pregnancy tests available on the market and their prices in Brazil are listed. Those based on the agglutination inhibition reactions can be used 8-10 days after determining the absence of menstruation, when the levels of chorionic gonadotropin range from 1500 to 3500 IU/1. A colorimetric test based on a chemical reaction is also available, and can be used 15 days after determining the absence of menstruation. The kits and their manufacturers are: Gravindex (Johnson), Teste de Gravidez (Roche), Pregnosticon Planoteste (Organon), Pregnosticon All-in (Organon), and Uni-test (Novaquimica) (colorimetric test).

  13. Comparação da glicemia em cães utilizando o glicosímetro portátil e o método de referência laboratorial.


    Grazielle Anahy de Sousa Aleixo


    O aumento ou a diminuição da taxa de glicose no sangue é uma alteração endócrina comum em seres humanos e animais, e que pode ocasionar graves conseqüências ao estado de saúde dos mesmos. Dosar a glicose sangüínea de pacientes portadores de hipo ou hiperglicemia permite que eles recebam adequado tratamento, propiciando uma melhor qualidade de vida. O presente trabalho teve como objetivo determinar os valores glicêmicos em cães utilizando um glicosímetro portátil (Accu-Chek Advantage / Roche D...

  14. Comparison of nested, multiplex, qPCR; FISH; SeptiFast and blood culture methods in detection and identification of bacteria and fungi in blood of patients with sepsis


    Gosiewski, Tomasz; Flis, Agnieszka; Sroka, Agnieszka; K?dzierska, Anna; Pietrzyk, Agata; K?dzierska, Jolanta; Drwi?a, Rafa?; Bulanda, Ma?gorzata


    Background Microbiological diagnosis of sepsis relies primarily on blood culture data. This study compares four diagnostic methods, i.e. those developed by us: nested, multiplex, qPCR (qPCR) and FISH with commercial methods: SeptiFast (Roche) (SF) and BacT/ALERT? 3D blood culture system (bioM?rieux). Blood samples were derived from adult patients with clinical symptoms of sepsis, according to SIRS criteria, hospitalized in the Intensive Care Unit. Results Using qPCR, FISH, SF, and culture, mi...

  15. Comparison of two automated assays of BTM (CTX and P1NP) and reference intervals in a Danish population

    DEFF Research Database (Denmark)

    Jørgensen, N R; Møllehave, L T; Hansen, Y B L


    -osteoporotic treatment were excluded, while subjects on hormonal contraceptives were included. RESULTS: There was significant disagreement between both the two P1NP assays with a mean difference of -3 μg/L (LoA -19 to 14) (p ... (pre-menopausal), and >30 years (post-menopausal) for women. CONCLUSIONS: There is significant disagreement between the IDS-iSYS and Roche Cobas assays for both reference markers. Consequently, the reference intervals for an adult, healthy population are different depending on the analysis method used...

  16. Fluid Regulation and Time Course of Erythropoietin during Multifactorial Strain of Austrian Special Forces Survival Training (United States)


    J Appl Physiol 19:829 - 832, 1964. 5. Baumgartner R.N., Chumela W.C., Roche A.F.: Bioelectrical impedance phase angle and body composition. Am J...34 day-’ (5760 kcal" day-’). Body composition was measured at TI by a bio-electrical impedance analysis (5) using a BIA 101- S analyser (RJL, Detroit...analysed the influence of prolonged physical exercise in marathon runners on EPO concentration before and several hours after a race. It was found that EPO

  17. Features of the mass transfer in magnetic cataclysmic variables with fast-rotating white dwarfs

    Directory of Open Access Journals (Sweden)

    Isakova Polina


    Full Text Available The flow structure in magnetic cataclysmic variables was investigated taking into account the effects of strong magnetic field and fast rotation of the white dwarf. We modeled the AE Aqr system as a unique object that has the rotation period of the white dwarf is about 1000 times shorter than the orbital period of the binary system. Observations show that in spite of fast rotation of the white dwarf some part of the stream from the inner Lagrange point comes into the Roche lobe region. We analyzed possible mechanisms preventing material to outflow from the system.

  18. Maximum mass ratio of AM CVn-type binary systems and maximum white dwarf mass in ultra-compact X-ray binaries

    Directory of Open Access Journals (Sweden)

    Arbutina Bojan


    Full Text Available AM CVn-type stars and ultra-compact X-ray binaries are extremely interesting semi-detached close binary systems in which the Roche lobe filling component is a white dwarf transferring mass to another white dwarf, neutron star or a black hole. Earlier theoretical considerations show that there is a maximum mass ratio of AM CVn-type binary systems (qmax ≈ 2/3 below which the mass transfer is stable. In this paper we derive slightly different value for qmax and more interestingly, by applying the same procedure, we find the maximum expected white dwarf mass in ultra-compact X-ray binaries.

  19. Analysis of conditional gene deletion using probe based Real-Time PCR

    Directory of Open Access Journals (Sweden)

    Lyko Frank


    Full Text Available Abstract Following publication of this article 1 the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science". The correct probe that was used for the 1lox/2lox allele ratio analysis in the paper is as follows Probe for 1lox/2lox allele quantification: 5'-6-FAM-atAaCtTCgtatagCATaCattatac-BHQ-1 -3' (uppercase letters = LNA bases Manufacturer: EUROGENTEC, Seraing, Belgium All other information and reaction conditions in the paper are correct as stated.

  20. Composite hot-subdwarf binaries -- I. The spectroscopically confirmed sdB sample


    Vos, Joris; Németh, Péter; Vučković, Maja; Østensen, Roy; Parsons, Steven


    Hot subdwarf-B (sdB) stars in long-period binaries are found to be on eccentric orbits, even though current binary-evolution theory predicts that these objects are circularized before the onset of Roche lobe overflow (RLOF). To increase our understanding of binary interaction processes during the RLOF phase, we started a long-term observing campaign to study wide sdB binaries. In this paper, we present a sample of composite binary sdBs, and the results of the spectral analysis of nine such sy...

  1. Flow cytometric assay detecting cytotoxicity against human endogenous retrovirus antigens expressed on cultured multiple sclerosis cells

    DEFF Research Database (Denmark)

    Møller-Larsen, A; Brudek, T; Petersen, T


    expressing increased amounts of human endogenous retrovirus antigens. MS patients also have increased antibody levels to these antigens. The target cells are spontaneously growing peripheral blood mononuclear cells (PBMCs) of B cell lineage, expressing human endogenous retrovirus HERV epitopes...... on their surface. Polyclonal antibodies against defined peptides in the Env- and Gag-regions of the HERVs were raised in rabbits and used in antibody-dependent cell-mediated cytotoxicity (ADCC) -assays. Rituximab® (Roche), a chimeric monoclonal antibody against CD20 expressed primarily on B cells, was used...

  2. Molecular Targeting of Prostate Cancer During Androgen Ablation: Inhibition of CHES1/FOXN3 (United States)


    prostate cancer cells. N. Xiang, J. K. Yang, J. M. Webb, C. B. Wee, D. L. Boucher, S. Y . Liu, C. A. Baron, R. W. de Vere White, J. P. Gregg, H. J. Kung... cancer agents mitomycin C and doxorubicin were shown to increase apoptosis susceptibility via a p53- dependent induction of the CD95 /APO-1/Fas death... cancer . Oncogene, 20: 7342-7351, 2001. 14. Huang, H., Cheville, J. C., Pan, Y ., Roche, P. C., Schmidt, L. J., and Tindall, D. J. PTEN induces

  3. Použití metody kvantifikace DNA jako screeningového nástroje pro efektivní genotypování vzorků ve forenzní DNA laboratoři.


    Koljenšič, Ivana


    Quantification of human DNA in forensic samples is an important step during STR profiling because the STR genotyping is sensitive to the quantity of DNA used in the PCR reaction. This study focuses on the importance of quantification in the entire process of genetic analysis. Two real time PCR platforms (Roche LightCycler480 System and ABI 7900 RT PCR) were used to compare two commercial kits in terms of DNA quantification. It was found out that accuracy of absolute quantification values in c...

  4. CMV information: print, online, phone, video. (United States)


    There are a number of treatment options available for cytomegalovirus (CMV) and approved preventive treatments for persons at high risk. Partnership in Vision has published a CMV Retinitis Report. A one-hour continuing medication education course entitled CMV Prophylaxis and Intraocular Therapy is available on the World Wide Web. The National Association of People with AIDS (NAPWA) has extended its CMV information hotline. Cidofovir is a new drug approved for marketing for CMV treatment. It is infused only once every two weeks. CMV videotapes are available from Hoffman-La Roche.

  5. EBSD-based investigations of upper mantle xenoliths, snowball garnets and advanced ceramics


    Vonlanthen, Pierre; Grobéty, Bernard


    L’automatisation au début des années ’90 de la technique de diffraction par électrons rétro-diffusés ou electron backscatter diffraction (EBSD) a complètement révolutionné les analyses de textures menées tant sur les roches que sur les matériaux industriels. Parmi plusieurs avancées majeures, cette méthode permet une détermination relativement rapide et simple de l’orientation cristallographique des grains contenus dans les solides polycristallins. L’intérêt est de taille non seulement dans l...

  6. Further considerations on contracting solar nebula

    International Nuclear Information System (INIS)

    Rawal, J.J.


    Prentice (1978) in his modern Laplacian theory of the origin of the solar system established the scenario of the formation of the solar system on the basis of the usual laws of conservation of mass and angular momentum and the concept of supersonic turbulent convection. This provides a physical understanding of the Titius-Bode law of planetary distances. Starting with the concept of the Roche limit, the author derives a similar relation and discusses Kepler's third law in relation to this concept. He also discusses the resonant structure in the solar system in this context. (Auth.)

  7. Prévalence des dyslipidémies au laboratoire de biochimie du CHU ...

    African Journals Online (AJOL)

    Le cholestérol total, le cholestérol HDL, le cholestérol LDL ainsi que les triglycérides ont été dosés grâce à des méthodes enzymatiques sur un automate de Biochimie de type Cobas Integra 400 (Roche Diagnostics). Résultats: la prévalence des dyslipidémies dans notre population d'étude est de 39,30%. Les prévalences ...

  8. Effects of rearing density on seabass (Dicentrarchus labrax, L. gene expression

    Directory of Open Access Journals (Sweden)

    M. Saroglia


    Full Text Available Animal welfare involves the subjective feeling of animals and the experience of pleasure, pain, frustration, hunger or other states and it is therefore difficult to define and to measure (Drawkins, 1990. When water quality criteria are respected and feeding restrictions are avoided, fish density may become one of the more relevant factors expected to potentially affect fish welfare. Such high density environment, may lead to a fish conditions that cannot be described or forecast simply with measurement of hematological responses (Roche and Bogé, 1996, in the same time, detection of zootechnical performances, do not provide a specific indications...

  9. Contribution à l'étude des phénomènes de transfert dans les milieux poreux fracturés


    BENOSMAN, Fayçal


    La production des hydrocarbures constitue une source d’énergie qui n’est pas inépuisable, ce qui oblige les industriels à développer de nouvelles techniques afin d’optimiser encore d’avantage leur production. L’une de ces méthodes est de fracturer la roche poreuse contenant ces fluides Nous entreprenons à travers ce travail de recherche, l’étude d’un écoulement (eau-air) diphasique ascendant vertical dans un canal complètement poreux contenant des fractures, dont l’objectif ...

  10. On the nature of the symbiotic binary CI Cygni

    International Nuclear Information System (INIS)

    Kenyon, S.J.; Oliversen, N.A.; Mikolajewska, J.; Mikolajewski, M.; Stencel, R.E.


    An analysis of ultraviolet and optical spectroscopy is presented for the symbiotic binary CI Cyg. This system contains an M5 II asymptotic branch giant Mg of about 1.5 solar mass, transfering material at a few times 0.00001 solar mass/yr into a large accretion disk surrounding a main-sequence star with Mh of about 0.5 solar mass. A boundary layer at the inner edge of the disk photoionizes a small nebula approximately confined to the Roche volume of the accreting star. An extended, more highly ionized region forms when material ejected from the disk interacts with the red giant wind. 115 refs

  11. The genome of the fire ant Solenopsis invicta

    DEFF Research Database (Denmark)

    Wurm, Yannick; Wang, John; Riba-Grognuz, Oksana


    Ants have evolved very complex societies and are key ecosystem members. Some ants, such as the fire ant Solenopsis invicta, are also major pests. Here, we present a draft genome of S. invicta, assembled from Roche 454 and Illumina sequencing reads obtained from a focal haploid male and his brothers......, a functional DNA methylation system, and a single putative telomerase ortholog. EST data indicate that this S. invicta telomerase ortholog has at least four spliceforms that differ in their use of two sets of mutually exclusive exons. Some of these and other unique aspects of the fire ant genome are likely...... linked to the complex social behavior of this species....

  12. Effects of radiation pressure on the equipotential surfaces in x-ray binaries

    International Nuclear Information System (INIS)

    Kondo, Y.; McCluskey, G.E. Jr.; Gulden, S.L.


    Equipotential surfaces incorporating the effect of radiation pressure were computed for the x-ray binaries Cen X-3, Cyg X-1 = HDE 226868, Vela XR-1 = 3U 0900-40 = HD 77581, and 3U 1700-37 = HD 153919. The topology of the equipotential surfaces is significantly affected by radiation pressure. In particular, the so-called critical Roche (Jacobian) lobes, the traditional figure 8's, do not exist. The effects of these results on modeling x-ray binaries are discussed

  13. The Role of ERG and CXCR4 in Prostate Cancer Progression (United States)


    phosphate- buffered saline, and total cellular proteins were extracted with buffer con-taining 62.5 mM Tris-HCl ( pH 6.8), 2% SDS, 1 mM PMSF, and 1...and growth in osseous sites in vivo. J Bone Miner Res 20, 318–329. [26] Murakami K, Mavrothalassitis G, Bhat NK, Fisher RJ, and Papas TS (1993...with buffer containing 62.5 mM Tris-HCl ( pH 6.8), 2% SDS, 1 mM PMSF, and 1X protease inhibitor cocktail (Roche, Indianapolis, IN); for IP studies

  14. Performance of a commercial assay for the diagnosis of influenza A (H1N1 infection in comparison to the Centers for Disease Control and Prevention protocol of real time RT-PCR

    Directory of Open Access Journals (Sweden)

    María G Barbás


    Full Text Available At the time of influenza A (H1N1 emergency, the WHO responded with remarkable speed by releasing guidelines and a protocol for a real-time RT-PCR assay (rRT-PCR. The aim of the present study was to evalúate the performance of the "Real Time Ready Influenza A/H1N1 Detection Set" (June 2009-Roche kit in comparison to the CDC reference rRT-PCR protocol. The overall sensitivity of the Roche assay for detection of the Inf A gene in the presence or absence of the H1 gene was 74.5 %. The sensitivity for detecting samples that were only positive for the Inf A gene (absence of the H1 gene was 53.3 % whereas the sensitivity for H1N1-positive samples (presence of the Inf A gene and any other swine gene was 76.4 %. The specificity of the assay was 97.1 %. A new version of the kit (November 2009 is now available, and a recent evaluation of its performance showed good sensitivity to detect pandemic H1N1 compared to other molecular assays.Durante la pandemia de influenza A (H1N1, la OMS recomendó algoritmos y protocolos de detección del virus mediante RT-PCR en tiempo real. El objetivo del presente estudio fue evaluar el desempeño del equipo que comercializa la empresa Roche, Real Time Ready Influenza A/H1N1 Detection Set (junio de 2009, en comparación con el protocolo de RT-PCR en tiempo real de los CDC. La sensibilidad global del ensayo de Roche para la detección del gen Inf A en presencia o ausencia del gen H1 fue 74,5 %. La sensibilidad para la detección de muestras positivas solo para el gen Inf A (ausencia del gen H1 fue 53,3 % y la sensibilidad para la detección de muestras positivas para H1N1 (presencia del gen Inf A y cualquier otro gen porcino fue 76,4 %. La especificidad fue 97,1 %. Existe una nueva versión del equipo (noviembre 2009 que, según se ha descrito, presenta buena sensibilidad en comparación con otros ensayos moleculares para detectar H1N1 pandémica.

  15. The Influence of Coronal Mass Ejections on the Mass-loss Rates of Hot-Jupiters

    Energy Technology Data Exchange (ETDEWEB)

    Cherenkov, A.; Bisikalo, D. [Institute of Astronomy of the Russian Academy of Sciences, 48 Pyatnitskaya St. 119017, Moscow (Russian Federation); Fossati, L.; Möstl, C., E-mail: [Space Research Institute, Austrian Academy of Sciences, Schmiedlstrasse 6, A-8042 Graz (Austria)


    Hot-Jupiters are subject to extreme radiation and plasma flows coming from their host stars. Past ultraviolet Hubble Space Telescope observations, supported by hydrodynamic models, confirmed that these factors lead to the formation of an extended envelope, part of which lies beyond the Roche lobe. We use gas-dynamic simulations to study the impact of time variations in the parameters of the stellar wind, namely that of coronal mass ejections (CMEs), on the envelope of the typical hot-Jupiter HD 209458b. We consider three CMEs characterized by different velocities and densities, taking their parameters from typical CMEs observed for the Sun. The perturbations in the ram-pressure of the stellar wind during the passage of each CME tear off most of the envelope that is located beyond the Roche lobe. This leads to a substantial increase of the mass-loss rates during the interaction with the CME. We find that the mass lost by the planet during the whole crossing of a CME is of ≈10{sup 15} g, regardless of the CME taken into consideration. We also find that over the course of 1 Gyr, the mass lost by the planet because of CME impacts is comparable to that lost because of high-energy stellar irradiation.

  16. Analysing 454 amplicon resequencing experiments using the modular and database oriented Variant Identification Pipeline

    Directory of Open Access Journals (Sweden)

    Deforce Dieter


    Full Text Available Abstract Background Next-generation amplicon sequencing enables high-throughput genetic diagnostics, sequencing multiple genes in several patients together in one sequencing run. Currently, no open-source out-of-the-box software solution exists that reliably reports detected genetic variations and that can be used to improve future sequencing effectiveness by analyzing the PCR reactions. Results We developed an integrated database oriented software pipeline for analysis of 454/Roche GS-FLX amplicon resequencing experiments using Perl and a relational database. The pipeline enables variation detection, variation detection validation, and advanced data analysis, which provides information that can be used to optimize PCR efficiency using traditional means. The modular approach enables customization of the pipeline where needed and allows researchers to adopt their analysis pipeline to their experiments. Clear documentation and training data is available to test and validate the pipeline prior to using it on real sequencing data. Conclusions We designed an open-source database oriented pipeline that enables advanced analysis of 454/Roche GS-FLX amplicon resequencing experiments using SQL-statements. This modular database approach allows easy coupling with other pipeline modules such as variant interpretation or a LIMS system. There is also a set of standard reporting scripts available.

  17. Performance evaluation of new automated hepatitis B viral markers in the clinical laboratory: two quantitative hepatitis B surface antigen assays and an HBV core-related antigen assay. (United States)

    Park, Yongjung; Hong, Duck Jin; Shin, Saeam; Cho, Yonggeun; Kim, Hyon-Suk


    We evaluated quantitative hepatitis B surface antigen (qHBsAg) assays and a hepatitis B virus (HBV) core-related antigen (HBcrAg) assay. A total of 529 serum samples from patients with hepatitis B were tested. HBsAg levels were determined by using the Elecsys (Roche Diagnostics, Indianapolis, IN) and Architect (Abbott Laboratories, Abbott Park, IL) qHBsAg assays. HBcrAg was measured by using Lumipulse HBcrAg assay (Fujirebio, Tokyo, Japan). Serum aminotransferases and HBV DNA were respectively quantified by using the Hitachi 7600 analyzer (Hitachi High-Technologies, Tokyo, Japan) and the Cobas AmpliPrep/Cobas TaqMan test (Roche). Precision of the qHBsAg and HBcrAg assays was assessed, and linearity of the qHBsAg assays was verified. All assays showed good precision performance with coefficients of variation between 4.5% and 5.3% except for some levels. Both qHBsAg assays showed linearity from 0.1 to 12,000.0 IU/mL and correlated well (r = 0.9934). HBsAg levels correlated with HBV DNA (r = 0.3373) and with HBcrAg (r = 0.5164), and HBcrAg also correlated with HBV DNA (r = 0.5198; P < .0001). This observation could provide impetus for further research to elucidate the clinical usefulness of the qHBsAg and HBcrAg assays.

  18. Hematological and Biochemical Markers of Iron Status in a Male, Young, Physically Active Population

    Directory of Open Access Journals (Sweden)

    Lázaro Alessandro Soares Nunes


    Full Text Available The aim of this study was to establish reference intervals (RIs for the hemogram and iron status biomarkers in a physically active population. The study population included male volunteers (n=150 with an average age of 19 ± 1 years who had participated in a regular and controlled exercise program for four months. Blood samples were collected to determine hematological parameters using a Sysmex XE-5000 analyzer (Sysmex, Kobe, Japan. Iron, total iron-binding capacity (TIBC, transferrin saturation and ferritin, and high-sensitivity C-reactive protein (CRP concentrations in serum samples were measured using commercial kits (Roche Diagnostics, GmbH, Mannheim, Germany and a Roche/Hitachi 902 analyzer. The RIs were established using the RefVal program 4.1b. The leucocyte count, TIBC, and CRP and ferritin concentrations exhibited higher RIs compared with those in a nonphysically active population. Thirty volunteers (outliers were removed from the reference population due to blood abnormalities. Among the outliers, 46% exhibited higher CRP concentrations and lower concentrations of iron and reticulocyte hemoglobin compared with the nonphysically active population (P<0.001. Our results showed that it is important to establish RIs for certain laboratory parameters in a physically active population, especially for tests related to the inflammatory response and iron metabolism.


    DEFF Research Database (Denmark)

    Tørring, Niels; aulesa, C; Eiben, Bernd


    their clinical and analytical performance. Patients and Methods We conducted a multicenter study in 5397 pregnancies including 108 cases with verified fetal T21 at 8 to 14 weeks of gestation. A technical validation of the Roche Elecsys® free βhCG and PAPP-A assays were performed, including method comparisons...... with the Brahms Kryptor®, PerkinElmer AutoDELFIA® and Siemens IMMULITE® assays. Furthermore a clinical validation including generation of assay specific medians from gestational age 8+0 to 14+0 weeks, and clinical test performance of risk assessment was performed. Results The imprecision of the Elecsys free βh...... translucency reached 94% for a 3% false positive rate. In gestational weeks 8 and 9 the sensitivity was 95% for a 2% false positive rate, and in gestational weeks 10 to 14 the sensitivity was 94% for a 3% false positive rate. Conclusions The technical and clinical validation of the Roche Elecsys free βh...

  20. Computer vision-based sorting of Atlantic salmon (Salmo salar) fillets according to their color level. (United States)

    Misimi, E; Mathiassen, J R; Erikson, U


    Computer vision method was used to evaluate the color of Atlantic salmon (Salmo salar) fillets. Computer vision-based sorting of fillets according to their color was studied on 2 separate groups of salmon fillets. The images of fillets were captured using a digital camera of high resolution. Images of salmon fillets were then segmented in the regions of interest and analyzed in red, green, and blue (RGB) and CIE Lightness, redness, and yellowness (Lab) color spaces, and classified according to the Roche color card industrial standard. Comparisons of fillet color between visual evaluations were made by a panel of human inspectors, according to the Roche SalmoFan lineal standard, and the color scores generated from computer vision algorithm showed that there were no significant differences between the methods. Overall, computer vision can be used as a powerful tool to sort fillets by color in a fast and nondestructive manner. The low cost of implementing computer vision solutions creates the potential to replace manual labor in fish processing plants with automation.

  1. Atlantic salmon skin and fillet color changes effected by perimortem handling stress, rigor mortis, and ice storage. (United States)

    Erikson, U; Misimi, E


    The changes in skin and fillet color of anesthetized and exhausted Atlantic salmon were determined immediately after killing, during rigor mortis, and after ice storage for 7 d. Skin color (CIE L*, a*, b*, and related values) was determined by a Minolta Chroma Meter. Roche SalmoFan Lineal and Roche Color Card values were determined by a computer vision method and a sensory panel. Before color assessment, the stress levels of the 2 fish groups were characterized in terms of white muscle parameters (pH, rigor mortis, and core temperature). The results showed that perimortem handling stress initially significantly affected several color parameters of skin and fillets. Significant transient fillet color changes also occurred in the prerigor phase and during the development of rigor mortis. Our results suggested that fillet color was affected by postmortem glycolysis (pH drop, particularly in anesthetized fillets), then by onset and development of rigor mortis. The color change patterns during storage were different for the 2 groups of fish. The computer vision method was considered suitable for automated (online) quality control and grading of salmonid fillets according to color.

  2. UV-induced mitotic co-segregation of genetic markers in Candida albicans: Evidence for linkage

    International Nuclear Information System (INIS)

    Crandall, M.


    Parasexual genetic studies of the medically important yeast Candida albicans were performed using the method of UV-induced mitotic segregation. UV-irradiation of the Hoffmann-La Roche type culture of C. albicans yielded a limited spectrum of mutants at a relatively high fequency. This observation suggested natural heterozygosity. Canavanine-sensitive (CanS) segregants were induced at a frequency of 7.6 . 10 -3 . Double mutants that were both CanS and methionine (Met - ) auxotrophs were induced at a frequency of 7.4 . 10 -3 . The single Met - segregant class was missing indicating linkage. UV-induced CanS or Met - CanS segregants occurred occasionally in twin-sectored colonies. Analyses of the sectors as well as the observed and missing classes of segregants indicated that genes met and can are linked in the cis configuration. The proposed gene order is: centromere - met - can. Thus, it is concluded that the Hoffmann-La Roche strain of C. albicans is naturally heterozygous at two linked loci. These findings are consistent with diploidy. (orig.)

  3. Resonant Tidal Forcing in Close Binaries: Implications for CVs (United States)

    Ford, K. E. Saavik; McKernan, Barry; Schwab, Elliana


    Resonant tidal forcing occurs when the tidal forcing frequency of a binary matches a quadrupolar oscillation mode of one of the binary members and energy is transferred from the orbit of the binary to the mode. Tidal locking permits ongoing resonant driving of modes even as binary orbital parameters change. At small binary separations during tidal lock, a significant fraction of binary orbital energy can be deposited quickly into a resonant mode and the binary decays faster than via the emission of gravitational radiation alone. Here we discuss some of the implications of resonant tidal forcing for the class of binaries known as Cataclysmic Variable (CV) stars. We show that resonant tidal forcing of the donor’s Roche lobe could explain the observed 2‑3hr period gap in CVs, assuming modest orbital eccentricities are allowed (eb ∼ 0.03), and can be complementary or an alternative to, existing models. Sudden collapse of the companion orbit, yielding a Type Ia supernova is disfavoured, since Hydrogen is not observed in Type Ia supernova spectra. Therefore, resonance must generally be truncated, probably via mass loss from the Roche lobe or orbital perturbation, ultimately producing a short period CV containing an ’overheated’ white dwarf.

  4. Advancing the understanding of progression in multiple sclerosis: an interview with Shibeshih Belachew. (United States)

    Belachew, Shibeshih


    Shibeshih Belachew speaks to Laura Dormer, Commissioning Editor: Dr Shibeshih Belachew, MD, PhD, is a Senior Medical Director for Multiple Sclerosis (MS) Disease Area in Global Product Development Medical Affairs at Roche (Basel, Switzerland). Prior to joining Roche in January 2016, he was Director of MS Franchise and Head of Medical Director's office for Biogen Region Europe and Canada. Previously at Biogen he also served as a Director in Global Neurology for the natalizumab program in Cambridge (MA, USA). Prior to joining industry, he was a Clinical Professor of Neurology at the University of Liège in Belgium. Shibeshih completed neurology postgraduate training at the University of Liège and has a PhD in Biomedical Science in the field of Developmental Neurobiology. Shibeshih has been a post-doctoral fellow in the Laboratory of Cellular and Synaptic Neurophysiology at the National Institutes of Health (Bethesda, MD, USA) and later at the Center for Neuroscience Research of Children's National Medical Center in Washington DC. He is a member of the Belgian Neurological Society.


    International Nuclear Information System (INIS)

    Fossati, L.; Haswell, C. A.; Patel, M. R.; Busuttil, R.; Bagnulo, S.; Kowalski, P. M.; Shulyak, D. V.; Sterzik, M. F.


    Since there are several ways planets can survive the giant phase of the host star, we examine the habitability and detection of planets orbiting white dwarfs. As a white dwarf cools from 6000 K to 4000 K, a planet orbiting at 0.01 AU would remain in the continuous habitable zone (CHZ) for ∼8 Gyr. We show that photosynthetic processes can be sustained on such planets. The DNA-weighted UV radiation dose for an Earth-like planet in the CHZ is less than the maxima encountered on Earth, and hence non-magnetic white dwarfs are compatible with the persistence of complex life. Polarization due to a terrestrial planet in the CHZ of a cool white dwarf (CWD) is 10 2 (10 4 ) times larger than it would be in the habitable zone of a typical M-dwarf (Sun-like star). Polarimetry is thus a viable way to detect close-in rocky planets around white dwarfs. Multi-band polarimetry would also allow us to reveal the presence of a planet atmosphere, providing a first characterization. Planets in the CHZ of a 0.6 M ☉ white dwarf will be distorted by Roche geometry, and a Kepler-11d analog would overfill its Roche lobe. With current facilities a super-Earth-sized atmosphereless planet is detectable with polarimetry around the brightest known CWD. Planned future facilities render smaller planets detectable, in particular by increasing the instrumental sensitivity in the blue.

  6. Improved Efficiency and Reliability of NGS Amplicon Sequencing Data Analysis for Genetic Diagnostic Procedures Using AGSA Software

    Directory of Open Access Journals (Sweden)

    Axel Poulet


    Full Text Available Screening for BRCA mutations in women with familial risk of breast or ovarian cancer is an ideal situation for high-throughput sequencing, providing large amounts of low cost data. However, 454, Roche, and Ion Torrent, Thermo Fisher, technologies produce homopolymer-associated indel errors, complicating their use in routine diagnostics. We developed software, named AGSA, which helps to detect false positive mutations in homopolymeric sequences. Seventy-two familial breast cancer cases were analysed in parallel by amplicon 454 pyrosequencing and Sanger dideoxy sequencing for genetic variations of the BRCA genes. All 565 variants detected by dideoxy sequencing were also detected by pyrosequencing. Furthermore, pyrosequencing detected 42 variants that were missed with Sanger technique. Six amplicons contained homopolymer tracts in the coding sequence that were systematically misread by the software supplied by Roche. Read data plotted as histograms by AGSA software aided the analysis considerably and allowed validation of the majority of homopolymers. As an optimisation, additional 250 patients were analysed using microfluidic amplification of regions of interest (Access Array Fluidigm of the BRCA genes, followed by 454 sequencing and AGSA analysis. AGSA complements a complete line of high-throughput diagnostic sequence analysis, reducing time and costs while increasing reliability, notably for homopolymer tracts.

  7. [Isolation and characterization of 27 polymorphic microsatellite markers in Armillaria luteo-virens ( Physalacriaceae)]. (United States)

    Xing, Rui; Gao, Qingbo; Zhang, Faqi; Li, Yinhu; Ful, Pengcheng; Zhang, Jinhua; Wang, Jiuli; Chen, Shilong


    [OBJECTIVE] The objectives of this study were to use Roche 454 GS FLX system to develop SSR markers for Armillaria luteo-virens. These datasets will be valuable for detecting genetic diversity and population structure of this species. [METHODS] We collected Armillaria luteo-virens samples from Yushu in Qinghai province, China. Total RNA was isolated by using the TRIzol reagent, after that we constructed cDNA library and performed one quarter plate of the whole run 454 pyrosequencing. We selected 98 primer pairs randomly from the 321 SSRs to evaluate their application and the polymorphism across 66 individuals (Armillaria luteo-virens) representing 3 wild populations. [RESULTS] Roche 454 sequencing yielded 197,121 reads with a total nucleotide size of 88,585,965bp. 27 of 98 SSRs loci were polymorphic. Numbers of alleles (Na) ranged from 2 to 8. Expected heterozygosity (HE) ranged from less than 0. 001 to 0. 810 at locus ALV65, while observed heterozygosity (Ho) from 0 at loci AIV64 and AIV92 to 0. 900 at loci ALV8. We found no evidence of linkage disequiliburium, however 10 of 27 SSR markers showed significant deviation from Hardy-weinberg equilibrium. [CONCLUSION] These remaining 17 pairs of Armillaria luteo-virens SSR markers will be valuable for future research on detecting population structure and conservation of this species.

  8. Comparison of commercial DNA preparation kits for the detection of Brucellae in tissue using quantitative real-time PCR

    Directory of Open Access Journals (Sweden)

    Straube Eberhard


    Full Text Available Abstract Background The detection of Brucellae in tissue specimens using PCR assays is difficult because the amount of bacteria is usually low. Therefore, optimised DNA extraction methods are critical. The aim of this study was to assess the performance of commercial kits for the extraction of Brucella DNA. Methods Five kits were evaluated using clinical specimens: QIAamp™ DNA Mini Kit (QIAGEN, peqGold™ Tissue DNA Mini Kit (PeqLab, UltraClean™ Tissue and Cells DNA Isolation Kit (MoBio, DNA Isolation Kit for Cells and Tissues (Roche, and NucleoSpin™ Tissue (Macherey-Nagel. DNA yield was determined using a quantitative real-time PCR assay targeting IS711 that included an internal amplification control. Results Kits of QIAGEN and Roche provided the highest amount of DNA, Macherey-Nagel and Peqlab products were intermediate whereas MoBio yielded the lowest amount of DNA. Differences were significant (p Conclusions We observed differences in DNA yield as high as two orders of magnitude for some samples between the best and the worst DNA extraction kits and inhibition was observed occasionally. This indicates that DNA purification may be more relevant than expected when the amount of DNA in tissue is very low.

  9. Erziehungstheorie, Literatur und Geschlecht Educational Theory, Literature, and Gender

    Directory of Open Access Journals (Sweden)

    Barbara Gribnitz


    Full Text Available Die Verfasserin stellt sich die Aufgabe, das „erkenntnistheoretische Potential des literarischen Textes als Quelle historisch-pädagogischer Forschung“ (S. 12 am Beispiel des Genre Bildungsroman herauszuarbeiten. Anhand zweier Romane, die weibliche Lebensläufe beschreiben (Sophie von La Roche: Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal, erweitert sie die Bildungsromandebatte in der Erziehungs- und Literaturwissenschaft um die Kategorie Geschlecht und bestimmt „den literarischen Text als privilegierten Ort dieser Auseinandersetzung“ (S. 40. Die Studie leidet unter Redundanz und oberflächlicher Lektüre der Texte.The author takes on the task of identifying the “epistemological potential of the literary text as source of historical-pedagogical research,” using the genre of the bildungsroman (12. Focusing on two novels that describe the course of female life histories (Sophie von La Roch: History of Lady Sophia Sternheim/Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal, she expands the debate on the bildungsroman in education and literary studies to include the categories of gender and identifies “the literary text as the privileged space for this conflict” (40. The study suffers under redundancy and superficial readings of the texts.

  10. Automated alarm to detect antigen excess in serum free immunoglobulin light chain kappa and lambda assays. (United States)

    Urdal, Petter; Amundsen, Erik K; Toska, Karin; Klingenberg, Olav


    Antigen excess causing a falsely low concentration result may occur when measuring serum free immunoglobulin light chains (SFLC). Automated antigen excess detection methods are available only with some analyzers. We have now developed and verified such a method. Residuals of sera with known SFLC-κ and -λ concentrations were analyzed using Binding Site reagents and methods adapted to the Roche Cobas® c.501 analyzer. We analyzed 117 sera for SFLC-κ and -λ and examined how the absorbance increased with time during the 7 minutes of reaction (absorbance reading points 12-70). From this an antigen excess alarm factor (ratio of absorbance increases between reading points 68-60 and 20-12, multiplied by 100) was defined. Upon our request, Roche added to our two SFLC assays a program which calculated this antigen excess alarm factor and triggered an alarm when the factor was below a defined value. We verified this antigen excess alarm function by analyzing serum from 325 persons of whom 143 were multiple myeloma patients. All samples with a known concentration above 30 mg/L triggered either an antigen excess alarm, an 'above test' alarm or both. Also, all samples above 200 mg/L (SFLC-λ) and 300 mg/L (SFLC-κ) triggered the antigen excess alarm and all but one triggered the above test alarm. The antigen excess alarm function presented here detected all known antigen excess samples at no increased time of analysis, a reduced workload and reduced reagent cost.

  11. A Simple Phosphate-Buffered-Saline-Based Extraction Method Improves Specificity of HIV Viral Load Monitoring Using Dried Blood Spots. (United States)

    Makadzange, A Tariro; Boyd, F Kathryn; Chimukangara, Benjamin; Masimirembwa, Collen; Katzenstein, David; Ndhlovu, Chiratidzo E


    Although Roche COBAS Ampliprep/COBAS TaqMan (CAP/CTM) systems are widely used in sub-Saharan Africa for early infant diagnosis of HIV from dried blood spots (DBS), viral load monitoring with this system is not practical due to nonspecific extraction of both cell-free and cell-associated viral nucleic acids. A simplified DBS extraction technique for cell-free virus elution using phosphate-buffered saline (PBS) may provide an alternative analyte for lower-cost quantitative HIV virus load (VL) testing to monitor antiretroviral therapy (ART). We evaluated the CAP/CTM v2.0 assay in 272 paired plasma and DBS specimens using the cell-free virus elution method and determined the level of agreement, sensitivity, and specificity at thresholds of target not detected (TND), target below the limit of quantification (BLQ) (1,000 copies/ml, the sensitivities, specificities, positive predictive values (PPV), and negative predictive values (NPV) were 92.7%, 100%, 100%, and 94.3%, respectively. PBS elution of DBS offers a sensitive and specific method for monitoring plasma viremia among adults and children on ART at the WHO-recommended threshold of >1,000 copies/ml on the Roche CAP/CTM system. Copyright © 2017 Makadzange et al.

  12. Dissection of the octoploid strawberry genome by deep sequencing of the genomes of Fragaria species. (United States)

    Hirakawa, Hideki; Shirasawa, Kenta; Kosugi, Shunichi; Tashiro, Kosuke; Nakayama, Shinobu; Yamada, Manabu; Kohara, Mistuyo; Watanabe, Akiko; Kishida, Yoshie; Fujishiro, Tsunakazu; Tsuruoka, Hisano; Minami, Chiharu; Sasamoto, Shigemi; Kato, Midori; Nanri, Keiko; Komaki, Akiko; Yanagi, Tomohiro; Guoxin, Qin; Maeda, Fumi; Ishikawa, Masami; Kuhara, Satoru; Sato, Shusei; Tabata, Satoshi; Isobe, Sachiko N


    Cultivated strawberry (Fragaria x ananassa) is octoploid and shows allogamous behaviour. The present study aims at dissecting this octoploid genome through comparison with its wild relatives, F. iinumae, F. nipponica, F. nubicola, and F. orientalis by de novo whole-genome sequencing on an Illumina and Roche 454 platforms. The total length of the assembled Illumina genome sequences obtained was 698 Mb for F. x ananassa, and ∼200 Mb each for the four wild species. Subsequently, a virtual reference genome termed FANhybrid_r1.2 was constructed by integrating the sequences of the four homoeologous subgenomes of F. x ananassa, from which heterozygous regions in the Roche 454 and Illumina genome sequences were eliminated. The total length of FANhybrid_r1.2 thus created was 173.2 Mb with the N50 length of 5137 bp. The Illumina-assembled genome sequences of F. x ananassa and the four wild species were then mapped onto the reference genome, along with the previously published F. vesca genome sequence to establish the subgenomic structure of F. x ananassa. The strategy adopted in this study has turned out to be successful in dissecting the genome of octoploid F. x ananassa and appears promising when applied to the analysis of other polyploid plant species.

  13. Serum and Plasma Neutrophil Gelatinase Associated Lipocalin (NGAL) Levels are Not Equivalent in Patients Admitted to Intensive Care

    DEFF Research Database (Denmark)

    Itenov, Theis Skovsgaard; Bangert, Kristian; Christensen, Per Hjort


    -enhanced turbidimetric immunoassay (The NGAL Test™, BioPorto Diagnostics A/S, Gentofte, Denmark) on a Roche Hitachi 917 (Roche-Hitachi, Inc., Tokyo, Japan) analyzer. RESULTS: Serum NGAL concentrations ranged from 26.8 to 1,808 ng/ml (median 281 ng/ml, interquartile range (IQR) 453 ng/ml). EDTA plasma NGAL concentrations......BACKGROUND: Neutrophil gelatinase associated lipocalin (NGAL) is proposed as a biomarker of acute kidney injury (AKI). NGAL has been studied in a range of body fluids including serum and EDTA plasma. The aim of the present study was to establish relationship between serum NGAL concentrations...... ranged from 25.7 to 1,752 ng/ml (median 225 ng/ml, IQR 352 ng/ml). The difference in NGAL concentrations in paired serum and EDTA plasma samples (serum- plasma) ranged from -13.8 to 321 ng/ml (median 79 ng/ml, IQR 116 ng/ml; difference from zero, P

  14. Elements of Poro-Elasticity for Reservoir Engineering Éléments de poro-élasticité pour l'ingénierie de réservoirs

    Directory of Open Access Journals (Sweden)

    Bouteca M.


    -élasticité utiles à l'ingénieur de réservoir. La poro-élasticité permet de calculer les déformations du solide liées au mouvement du fluide. C'est donc le cadre théorique qui permet de définir la compressibilité de la roche. Nous montrons ainsi, dans un premier temps, comment les variables mécaniques précédemment introduites sont reliées à la compressibilité de la roche mesurée expérimentalement. Deux hypothèses sont retenues : a mesure en condition isotrope (définie par une pression de confinement et une pression de pore, b mesure en condition oedométrique (définie par un chargement vertical, un déplacement radial nul et une pression de pore. Les principaux résultats sont illustrés dans le tableau 6. On montre en outre qu'en condition isotrope, la compressibilité du volume poreux peut être mesurée indifféremment à pression de pore variable et pression de confinement constante ou à pression de pore constante et pression de confinement variable (tableau 5. Dans un deuxième temps nous examinons sous quelles conditions l'équation de diffusivité définie dans la première partie peut être découplée de l'équation d'équilibre du solide. On montre que la contribution de la déformation de la roche à l'écoulement peut être exprimée à l'aide d'un coefficient de compressibilité à condition que l'on fasse des hypothèses sur le mode de déformation local de la roche. Rappelons que, par opposition à des conditions aux limites qui sont vraies sur le contour du domaine, les conditions locales sont vraies en tout point et constituent une simplification du problème réel. En conclusion, on souligne l'importance du choix des conditions locales retenues pour la mesure de la compressibilité de la roche. La compressibilité de la roche étant définie, l'équation de diffusivité prend sa forme classique : la contribution de la roche est introduite en définissant une compressibilité totale somme de la compressibilité du fluide et de celle de la roche.

  15. Sensibilidad del equipo Cobas AmpliScreenTM HIV-1 Test, v1.5, para la detección de HIV-1

    Directory of Open Access Journals (Sweden)

    Lucía P Gomez

    Full Text Available Las técnicas de amplificación de ácidos nucleicos (NAT se incorporaron en los bancos de sangre para reducir el riesgo residual de transmisión de infecciones por vía transfusional. La cocirculación de distintas variantes del HIV-1 en Argentina indica la necesidad de evaluar la sensibilidad de los ensayos serológicos y moleculares disponibles para su detección. En este trabajo se evaluó la sensibilidad del equipo COBAS AmpliScreenTM HIV-1 Test, versión 1.5 (Roche, para detectar ARN viral en plasmas de individuos infectados con HIV-1 de Argentina. Los resultados demuestran que esta técnica tiene una alta sensibilidad para detectar ARN de HIV-1 en las condiciones ensayadas: para ensayo de mini-pooles (pooles = 50 copias de ARN/ml, la sensibilidad fue = 92 %, y para procedimiento estándar (plasmas = 207 copias de ARN/ml, la sensibilidad fue 100 %. Además, la técnica COBAS AmpliScreenTM HIV-1 Test, versión 1.5 (Roche, es adecuada para la detección de las variantes de HIV-1 prevalentes.

  16. LUT Reveals a New Mass-transferring Semi-detached Binary (United States)

    Qian, S.-B.; Zhou, X.; Zhu, L.-Y.; Zejda, M.; Soonthornthum, B.; Zhao, E.-G.; Zhang, J.; Zhang, B.; Liao, W.-P.


    GQ Dra is a short-period eclipsing binary in a double stellar system that was discovered by Hipparcos. Complete light curves in the UV band were obtained with the Lunar-based Ultraviolet Telescope in 2014 November and December. Photometric solutions are determined using the W-D (Wilson and Devinney) method. It is discovered that GQ Dra is a classical Algol-type semi-detached binary where the secondary component is filling the critical Roche lobe. An analysis of all available times of minimum light suggests that the orbital period is increasing continuously at a rate of \\dot{P}=+3.48(+/- 0.23)× {10}-7 days yr-1. This could be explained by mass transfer from the secondary to the primary, which is in agreement with the semi-detached configuration with a lobe-filling secondary. By assuming a conservation of mass and angular momentum, the mass transfer rate is estimated as \\dot{m}=9.57(+/- 0.63)× {10}-8 {M}⊙ {{yr}}-1. All of these results reveal that GQ Dra is a mass-transferring semi-detached binary in a double system that was formed from an initially detached binary star. After the massive primary evolves to fill the critical Roche lobe, the mass transfer will be reversed and the binary will evolve into a contact configuration with two sub-giant or giant component stars.

  17. Correction to: Tocilizumab: A Review in Rheumatoid Arthritis. (United States)

    Scott, Lesley J


    The article Tocilizumab: A Review in Rheumatoid Arthritis, written by Lesley J. Scott, was originally published Online First without open access. After publication in volume 77, issue 17, pages 1865-1879 F. Hoffmann-La Roche Ltd requested that the article be Open Choice to make the article an open access publication. Post-publication open access was funded by F. Hoffmann-La Roche Ltd. Further details may be found at . The article is forthwith distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( ), which permits any noncommercial use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

  18. OSI-774 OSI Pharmaceuticals. (United States)

    Norman, P


    OSI-774 (formerly CP-358774), a quinazoline derivative, is an orally active epidermal growth factor receptor (EGFR) inhibitor which was originally under joint development by Pfizer and OSI Pharmaceuticals (formerly Oncogene Science) for the potential treatment of cancer (eg, ovarian, non-small cell lung cancer (NSCLC) and head and neck). It is being evaluated in phase II trials [304305], [372201]. On 8 January 2001, OSI announced that it had signed an agreement with Roche and Genentech for the global co-development and marketing of OSI-774. The agreement with Genentech covers the United States, that with Roche the rest of the world [395371], [395526]. In June 2000, OSI gained all development and marketing rights for OSI-774 following Pfizer's merger with Warner-Lambert [371439]. In September 2000, Pfizer transferred the IND dossierfor OSI-774 to OSI ahead of the timeline agreed in the June 2000 development and marketing rights agreement [383786]. The phase II trials will assess OSI-774 both as a single agent and in combination with existing chemotherapy regimens [347783]. Phase III trials are expected to be initiated in 2001 [347783]. In October 2000, Lehman Brothers predicted that OSI-774 would move into pivotal trials in thefirst half of 2001 and that the drug would be launched in 2003. The analysts also estimated worldwide sales of US $66 million, $285 million and $461 million in 2003, 2004 and 2005, respectively, and peak sales in excess of US $500 million [395189].

  19. GW170817: a neutron star merger in a mass-transferring triple system (United States)

    Chang, Philip; Murray, Norman


    The light curve of GW170817 is surprisingly blue and bright. Assuming that the event is a binary neutron star merger, we argue that blueness and brightness of the light curve is the result of ejecta that contains an substantial amount of thermal energy. To achieve this, the ejecta must be reheated at a substantial distance (1-2000 solar radii) from the merger to avoid losing the energy to adiabatic cooling. We show that this reheating can occur if the merger occurs in a hierarchical triple system where the outer star has evolved and filled its Roche lobe. The outer star feeds mass to the inner binary, forming a circumbinary disc, driving the inner binary to merge. Because the outer star fills its Roche lobe, a substantial fraction of the dynamical ejecta collides with the evolved star, reheating the ejecta in the process. We suggest that the process of mass transfer in hierarchical triples tends to form coplanar triple systems such as PSR J0337+1715, and may provide electromagnetic counterparts to binary black hole mergers.

  20. Use of reverse-transcriptase-based HIV-1 viral load assessment to confirm low viral loads in newly diagnosed patients in Switzerland. (United States)

    Vetter, Beatrice N; Shah, Cyril; Huder, Jon B; Böni, Jürg; Schüpbach, Jörg


    Treatment-naïve patients newly diagnosed with HIV occasionally present with low viral RNA of ≤1'000 copies/ml, raising concerns about viral load underestimation. Because falsely low or undetectable viral loads might lead to inadvertent virus transmission or treatment delays, confirmation of such cases by a sequence-independent viral load test is recommended in Switzerland. HIV-1 RNA ≤1'000 cp/ml by Roche's or Abbott's tests in patients newly diagnosed from 2010 to 2012 in Switzerland were subjected to viral load testing by the product-enhanced-reverse transcriptase (PERT) assay. These investigations were complemented with repeat and/or alternative viral RNA measurements. HIV-1 RNA ≤1'000 cp/ml was observed in 71 of 1814 newly diagnosed patients. The PERT assay suggested clinically relevant viral load underestimation in 7 of 32 cases that could be investigated. In four patients, the PERT viral load was 10-1'000-fold higher; this was confirmed by alternative HIV-1 RNA tests. Six of the 7 underestimates had been obtained with meanwhile outdated versions of Roche's HIV-1 RNA test. In the seventh patient, follow-up revealed similar results for RNA and PERT based viral loads. PERT assay revealed occasional severe viral load underestimation by versions of HIV-1 RNA tests meanwhile outdated. Underestimation by contemporary tests appears rare, however.