Sample records for amplicor roche

  1. Comparison of the Becton Dickinson strand displacement amplification and Cobas Amplicor Roche PCR for the detection of Chlamydia trachomatis: pooling versus individual tests

    DEFF Research Database (Denmark)

    Bang, D; Angelsø, Lene; Schirakow, Bente


    The objective of the study was to examine the influence of pooling Chlamydia trachomatis specimens. We compared Becton Dickinson ProbeTec strand displacement amplification (SDA) with Cobas Amplicor Roche (PCR). With PCR as the standard, SDA performed equally well in single-sample testing....... For pooled PCR samples (compared to individual PCR), we found a sensitivity of 100% and a specificity of 98.9%. For pooled SDA tests (compared to individual SDA), we found a sensitivity of 86.5% and a specificity of 98.9%. Our conclusion is that 2-sucrose phosphate buffer (2-SP) can be used for individual...

  2. Pooling Ocular Swab Specimens from Tanzania for testing by Roche Amplicor and Aptima Combo 2 Assays for the detection of Chlamydia trachomatis: Accuracy and Cost Savings (United States)

    Dize, Laura; West, Sheila; Quinn, Thomas C.; Gaydos, Charlotte A.


    Ocular swabs collected in Tanzania were evaluated by Amplicor CT and Aptima Combo2 assays for the detection of Chlamydia trachomatis (CT) to determine if pooling could be used to reduce the cost of detection. Pooling would be an accurate method and so far resulted in a cost-savings of 62.2%. PMID:24079951

  3. Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma. (United States)

    Murphy, D G; Côté, L; Fauvel, M; René, P; Vincelette, J


    The performance and characteristics of Roche COBAS AMPLICOR HIV-1 MONITOR version 1.5 (CA MONITOR 1.5) UltraSensitive (usCA MONITOR 1. 5) and Standard (stCA MONITOR 1.5) procedures, Organon Teknika NucliSens HIV-1 RNA QT with Extractor (NucliSens), and Bayer Quantiplex HIV RNA version 3.0 (bDNA 3.0) were compared in a multicenter trial. Samples used in this study included 460 plasma specimens from human immunodeficiency virus (HIV) type 1 (HIV-1)-infected persons, 100 plasma specimens from HIV antibody (anti-HIV)-negative persons, and culture supernatants of HIV-1 subtype A to E isolates diluted in anti-HIV-negative plasma. Overall, bDNA 3.0 showed the least variation in RNA measures upon repeat testing. For the Roche assays, usCA MONITOR 1.5 displayed less variation in RNA measures than stCA MONITOR 1.5. NucliSens, at an input volume of 2 ml, showed the best sensitivity. Deming regression analysis indicated that the results of all three assays were significantly correlated (P < 0.0001). However, the mean difference in values between CA MONITOR 1.5 and bDNA 3.0 (0.274 log(10) RNA copies/ml; 95% confidence interval, 0.192 to 0.356) was significantly different from 0, indicating that CA MONITOR 1.5 values were regularly higher than bDNA 3.0 values. Upon testing of 100 anti-HIV-negative plasma specimens, usCA MONITOR 1.5 and NucliSens displayed 100% specificity, while bDNA 3.0 showed 98% specificity. NucliSens quantified 2 of 10 non-subtype B viral isolates at 1 log(10) lower than both CA MONITOR 1.5 and bDNA 3.0. For NucliSens, testing of specimens with greater than 1,000 RNA copies/ml at input volumes of 0.1, 0.2, and 2.0 ml did not affect the quality of results. Additional factors differing between assays included specimen throughput and volume requirements, limit of detection, ease of execution, instrument work space, and costs of disposal. These characteristics, along with assay performance, should be considered when one is selecting a viral load assay.

  4. Viral load: Roche applies for marketing approval for ultrasensitive test. (United States)


    Roche Molecular Systems has applied for FDA permission to market a more sensitive viral load test. The Amplicor HIV-1 Monitor UltraSensitive Method tests viral load as low as 50 copies; current tests are only accurate to 400 copies. There is a widespread consensus among physicians that testing below 400 copies would be a valuable treatment tool.

  5. Comparison of the COBAS AMPLICOR MTB and BDProbeTec ET assays for detection of Mycobacterium tuberculosis in respiratory specimens.

    NARCIS (Netherlands)

    W.H.F. Goessens (Wil); P. de Man (Peter); J.G. Koeleman; A. Luijendijk (Ad); R. te Witt (René); H.P. Endtz (Hubert); A.F. van Belkum (Alex)


    textabstractThe performances of the BDProbeTec ET (Becton Dickinson) and COBAS AMPLICOR MTB (Roche) were retrospectively evaluated for detecting Mycobacterium tuberculosis complex in various respiratory specimens. The BACTEC and MGIT liquid culture system (Becton Dickinson) was used as a reference m

  6. Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR. (United States)

    Farrell, D J


    Certain strains of Neisseria subflava and Neisseria cinerea are known to produce false-positive results with the AMPLICOR Neisseria gonorrhoeae PCR (Roche Diagnostic Systems, Branchburg, N.J.). The analytical sensitivity and analytical specificity of three PCR tests were assessed with 3 geographically diverse N. gonorrhoeae strains and 30 non-N. gonorrhoeae Neisseria spp. The sensitivities of the in-house nested cppB gene and the 16S rRNA PCR methods were greater than that of the AMPLICOR N. gonorrhoeae PCR with purified DNA from all 3 N. gonorrhoeae strains. Six of 14 clinical strains of N. subflava (1 from a vaginal swab, 5 from respiratory sites) produced false-positive AMPLICOR N. gonorrhoeae PCR results and were negative by the two other PCR methods. When applied to 207 clinical specimens selected from a population with a high prevalence ( approximately 9%) of infection, the results for 15 of 96 (15.6%) AMPLICOR-positive specimens and 14 of 17 (82.3%) AMPLICOR-equivocal specimens were not confirmed by the more sensitive nested cppB PCR method. Only 2 of 94 (2.1%) of AMPLICOR N. gonorrhoeae PCR-negative specimens from the same population tested positive by the nested cppB method. These results suggest that for this population the AMPLICOR N. gonorrhoeae PCR test is suitable as a screening test only and all positive results should be confirmed by a PCR method that is more specific and at least as sensitive. This study also illustrates that caution should be used when introducing commercially available nucleic acid amplification-based diagnostic tests into the regimens of tests used for populations not previously tested with these products.

  7. Reproducibility of AMPLICOR enterovirus PCR test results.



    The reproducibility of AMPLICOR enterovirus PCR test results was determined with clinical samples of cerebrospinal fluid, serum, urine, and throat and rectal swabs. Among 608 samples from which duplicate aliquots were run simultaneously, only seven pairs gave discordant results. Among 104 samples from which duplicate aliquots were run in separate assays, no discordance was seen. Overall, the reproducibility of test kit results was 99% (705 of 712).

  8. Homage to Jean Roche

    CERN Multimedia


    It was with deep sadness that we learnt that Jean Roche had died. Jean was one of those quietly efficient and conscientious pillars of our community who beaver away unobtrusively for decades and make such a contribution to building CERN. An expert in thermal engineering, he left his mark on many buildings on the Meyrin and Prévessin sites. At the time of the LEP project at the beginning of the 1980s he was in charge of defining the principles of the ventilation systems for the new accelerator. Through his design studies and the calls for tenders that he launched, he developed these high-performance state-of-the-art installations which not only made LEP’s successes possible but also served as benchmarks for the fundamental principles underpinning the design of the LHC ventilation system. Jean always liked to surround himself with young people and during the years of his stewardship the design office was a splendid training ground. Despite his responsibilities, Jean was always keen t...

  9. A Java Interface for Roche Lobe Calculations (United States)

    Leahy, D. A.; Leahy, J. C.


    A JAVA interface for calculating various properties of the Roche lobe has been created. The geometry of the Roche lobe is important for studying interacting binary stars, particularly those with compact objects which have a companion which fills the Roche lobe. There is no known analytic solution to the Roche lobe problem. Here the geometry of the Roche lobe is calculated numerically to high accuracy and made available to the user for arbitrary input mass ratio, q.

  10. Detection of PCR inhibitors in cervical specimens by using the AMPLICOR Chlamydia trachomatis assay

    NARCIS (Netherlands)

    R.P.A.J. Verkooyen (Roel); A. Luijendijk (Ad); W.M. Huisman; W.H.F. Goessens (Wil); J.A.J.W. Kluytmans (Jan); J.H. van Rijsoort-Vos; H.A. Verbrugh (Henri)


    textabstractTo determine that susceptibility of AMPLICOR Chlamydia trachomatis PCR to inhibitory factors possibly present in cervical specimens, we obtained cervical specimens from 200 gynecology patients attending our outpatient clinic. The prevalence of C. trachomatis

  11. Performance of Roche CAP/CTM HIV-1 qualitative test version 2.0 using dried blood spots for early infant diagnosis. (United States)

    Gueye, Sokhna Bousso; Diop-Ndiaye, Halimatou; Diallo, Mamadou Malick; Ly, Omar; Sow-Ndoye, Aissatou; Diagne-Gueye, Ndèye Diabou; Kébé-Fall, Khady; Diop, Fatou; Gaye-Diallo, Aïssatou; Belec, Laurent; Mboup, Souleymane; Touré-Kane, Coumba


    In the context of early infant diagnosis (EID) decentralization in sub-Saharan Africa, dried blood spot (DBS) is now widely used for HIV proviral DNA detection in resource-limited settings. A new version of CAP/CTM (version 2) has been introduced, recently by Roche Diagnosis as a new real-time PCR assay to replace previous technologies on qualitative detection of HIV-1 DNA using whole blood and DBS samples. The objective of this study was to evaluate CAP/CTM version 2 compared to CAP/CTM version 1 and Amplicor on DBS. A total of 261 DBS were collected from children aged 4 weeks to 17 months born from HIV-seropositive mothers and tested by the three techniques. CAP/CTM version 2 showed 100% of agreement with Amplicor including 74 positive results and 187 negative results. CAP/CTM version 2 versus CAP/CTM version 1 as well as CAP/CTM version 1 versus Amplicor showed two discordant results giving a sensitivity of 98.6%, specificity of 99.5%, positive predictive value of 98.6% and negative predictive value of 99.5%. The concordance was 99.12% (95% of confidence interval) giving a Kappa coefficient of 0.97 (pHIV-1 EID.

  12. Roch Carrier, Popular Language, and Joual. (United States)

    Walkley, Max


    Discusses the language problems in Roch Carrier's French Canadian short stories, as well as his concise, nostalgic writing style. The article gives examples of vocabulary in his characters' dialogue; reviews the evolvement of French in Quebec and the question of "joual," in particular; and focuses on expressions posing difficulty for…

  13. Comparison of the COBAS/Ampliprep Taqman and Amplicor HIV-1 monitor tests in Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    Oluemi S. Amoo


    Full Text Available Background: The use of real-time Polymerase chain reaction (PCR technology options is increasing in resource-limited settings because they are faster, improve assay sensitivity,have higher throughput, larger dynamic ranges and reduced rates of contamination. In 2010, UNAIDS ranked Nigeria as the second highest population of people living with HIV and AIDS (2.98 million people in the world.Objective: The objective of this study was to compare the analytical performances of the Amplicor HIV-1 Monitor (version 1.5 and the COBAS Ampliprep/Taqman (version 2.0 usedin monitoring HIV disease progression in HIV-infected individuals.Method: In a cross-sectional study, HIV-1 RNA values obtained with the Amplicor HIV-1 monitor version 1.5 were compared with those of the COBAS/Ampliprep TaqMan HIV-1version 2.0 in a routine clinical setting. Between May and November 2011, 176 plasma samples collected were analysed in parallel using both techniques. Data analysis was done using statgraphics Centurion XVI and Medcalc version 12.0.Result: The correlation coefficient for the two assays was 0.83 and the level of agreement using a Bland–Altman plot was 94.2%.Conclusion: These findings suggest that the results from the two methods were comparable, hence the COBAS/Ampliprep Taqman version 2.0 is recommended for high-volume laboratories.

  14. Linnugripp tõi ravimifirmale Roche magusa kasumi / Erik Aru

    Index Scriptorium Estoniae

    Aru, Erik


    Šveitsi ravimitootja Roche'i viiruseravimi Tamiflu müük on kasvanud. Roche kavatseb järgmiseks suveks suurendada toodangut võrreldes 2004. aastaga kaheksa korda, kuid seda ei peeta piisavaks. ÜRO ja WHO nõuavad, et firma loobuks patentidest või annaks koopiaravimite valmistajatele tootmise litsentsi. Tabel: F. Hoffmann-La Roche. Lisa: Linnugripi vaktsiini võib loota kuue kuu pärast

  15. Diagnostic value of Cobas Amplicor MTB and Rotorgene Real Time PCR for tuberculous meningitis: A six-year retrospective study

    Directory of Open Access Journals (Sweden)

    Gülnur Tarhan


    Full Text Available Objective: Tuberculous meningitis (TBM is the most severe and lethal form of tuberculosis (TB.Bacteriologic confirmation of TBM is difficult and slow. Therefore, most patients receive ntituberculosis treatment based only on clinical and cerebrospinal fluid (CSF characteristics. Rapid diagnosis of TBM is important to decrease morbidity and mortality. The aim of the study was to demonstrate that COBAS Amplicor MTB and Rotorgene Real Time (RT PCR is a rapid method of diagnosing TBM before and after initiating anti-tuberculosis treatment. Methods: A retrospective study was conducted between December 2002 and January 2009 in 468 patients with suspected TBM. Clinical specimens were collected from different hospitals in Ankara. All specimens were evaluated by smear microscopy and culture methods with Lowenstein-Jensen (LJ and MGIT culture system. Results: Using culture results as a gold standard, the sensitivity, specificity, positive predictive values (PPV, and negative predictive values (NPV were 71.0%, 98.8%, 97.8% and 75.0%, respectively, for COBAS Amplicor MTB and 80%, 98.9%, 99.0% and 80.0%, respectively, for Rotorgene RT PCR. Statistical analysis showed no significant differences between the COBAS Amplicor MTB and Rotorgene RT PCR (p≥0.05. All isolates were susceptible to isoniazid, rifampin, streptomycin, and ethambutol with proportion method in LJ medium. All isolates were defined as LAM7-TUR by spoligotyping. Conclusion: Retrospective analysis of COBAS Amplicor MTB and Rotorgene RT PCR found that both tests are effective in rapidly diagnosing MTB using CSF. It was concluded that Rotorgene RT PCR test is more sensitive (81.0% than COBAS Amplicor MTB (71.0%. J Microbiol Infect Dis 2015;5(4: 156-161

  16. Ice cream and orbifold Riemann-Roch (United States)

    Buckley, Anita; Reid, Miles; Zhou, Shengtian


    We give an orbifold Riemann-Roch formula in closed form for the Hilbert series of a quasismooth polarized n-fold (X,D), under the assumption that X is projectively Gorenstein with only isolated orbifold points. Our formula is a sum of parts each of which is integral and Gorenstein symmetric of the same canonical weight; the orbifold parts are called ice cream functions. This form of the Hilbert series is particularly useful for computer algebra, and we illustrate it on examples of {K3} surfaces and Calabi-Yau 3-folds. These results apply also with higher dimensional orbifold strata (see [1] and [2]), although the precise statements are considerably trickier. We expect to return to this in future publications.

  17. An integral Riemann-Roch theorem for surface bundles

    DEFF Research Database (Denmark)

    Madsen, Ib Henning


    This paper is a response to a conjecture by T. Akita about an integral Riemann–Roch theorem for surface bundles.......This paper is a response to a conjecture by T. Akita about an integral Riemann–Roch theorem for surface bundles....

  18. Roche volume filling and the dissolution of open star clusters

    CERN Document Server

    Ernst, A; Just, A; Noel, T


    From direct N-body simulations we find that the dynamical evolution of star clusters is strongly influenced by the Roche volume filling factor. We present a parameter study of the dissolution of open star clusters with different Roche volume filling factors and different particle numbers. We study both Roche volume underfilling and overfilling models and compare with the Roche volume filling case. We find that in the Roche volume overfilling limit of our simulations two-body relaxation is no longer the dominant dissolution mechanism but the changing cluster potential. We call this mechnism "mass-loss driven dissolution" in contrast to "two-body relaxation driven dissolution" which occurs in the Roche volume underfilling regime. We have measured scaling exponents of the dissolution time with the two-body relaxation time. In this experimental study we find a decreasing scaling exponent with increasing Roche volume filling factor. The evolution of the escaper number in the Roche volume overfilling limit can be d...

  19. Reliability of Cobas Amplicor PCR test in detection of Mycobacterium tuberculosis in respiratory and nonorespiratory specimens

    Directory of Open Access Journals (Sweden)

    Lepšanović Zorica


    Full Text Available Background/Aim. Traditional methods for detection of mycobacteria, such as microscopic examination for the presence of acid-fast bacilli and isolation of the organism by culture, have either a low sensitivity and/or specificity, or take weeks before a definite result is available. Molecular methods, especially those based on nucleic acid amplification, are rapid diagnostic methods which combine high sensitivity and high specificity. The aim of this study was to determine the usefulness of the Cobas Amplicor Mycobacterium tuberculosis polymerase chain reaction (CAPCR assay in detecting the tuberculosis cause in respiratory and nonrespiratory specimens (compared to culture. Methods. Specimens were decontaminated by the N-acetyl-L-cystein- NaOH method. A 500 μL aliquot of the processed specimen were used for inoculation of Löwenstein-Jensen (L-J slants, a drop for acid-fast staining, and 100 μL for PCR. The Cobas Amplicor PCR was performed according to the manufacturer's instructions. Results. A total of 110 respiratory and 355 nonrespiratory specimens were investigated. After resolving discrepancies by reviewing medical history, overall sensitivity, specificity, and positive and negative predictive values for CA-PCR assay compared to culture, were 83%, 100%, 100%, and 96.8%, respectively. In comparison, they were 50%, 99.7%, 87.5%, and 98%, respectively, for the nonrespiratory specimens. The inhibition rate was 2.8% for respiratory, and 7.6% for nonrespiratory specimens. Conclusion. CA-PCR is a reliable assay that enables specialists to start treatment promptly on a positive test result. Lower value for specificity in a group of nonrespiratory specimens is a consequence of an extremely small number of mycobacteria in some of them.

  20. A Riemann-Roch theorem for the noncommutative two torus (United States)

    Khalkhali, Masoud; Moatadelro, Ali


    We prove the analogue of the Riemann-Roch formula for the noncommutative two torus Aθ = C(Tθ2)equipped with an arbitrary translation invariant complex structure and a Weyl factor represented by a positive element k ∈C∞(Tθ2). We consider a topologically trivial line bundle equipped with a general holomorphic structure and the corresponding twisted Dolbeault Laplacians. We define a spectral triple (Aθ , H , D) that encodes the twisted Dolbeault complex of Aθ and whose index gives the left hand side of the Riemann-Roch formula. Using Connes' pseudodifferential calculus and heat equation techniques, we explicitly compute the b2 terms of the asymptotic expansion of Tr(e-tD2) . We find that the curvature term on the right hand side of the Riemann-Roch formula coincides with the scalar curvature of the noncommutative torus recently defined and computed in Connes and Moscovici (2014) and independently computed in Fathizadeh and Khalkhali (2014).

  1. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.


    We construct linear network codes utilizing algebraic curves over finite fields and certain associated Riemann-Roch spaces and present methods to obtain their parameters. In particular we treat the Hermitian curve and the curves associated with the Suzuki and Ree groups all having the maximal...

  2. Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture

    NARCIS (Netherlands)

    Luijt, D.S.; Bos, P.A.; van Zwet, A.A.; Voorst-Vader, P.C.; Schirm, J.


    : A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased

  3. Comparison of COBAS AMPLICOR Neissefia gonorrhoeae PCR, including confirmation with N-gonorrhoeae-specific 16S rRNA PCR, with traditional culture

    NARCIS (Netherlands)

    Luijt, DS; Bos, PAJ; van Zwet, AA; Vader, PCV; Schirm, J


    A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased t

  4. ["Wetlands". Charles Bukowski and Charlotte Roche on hemorrhoids]. (United States)

    Bahmer, F A; Bahmer, J A


    More than 40 years ago Charles Bukowski described his experiences with coloscopy and the ensuing surgery on his hemorrhoids, both performed by a doctor pictured as sadistic. Bukowski not only depicts these procedures but also characterizes his compassionate inpatients as well as the nursing staff. In her bestseller published in 2008 Charlotte Roche's protagonist Helen needs surgical inpatient treatment because of hemorrhoidal bleeding. Her stay in the hospital, prolonged by a postoperative self-inflicted bleeding, provides the basis not only for strategies to bring her divorced parents together but also for thoughts on genitalia, manifold sexual practices, as well as on rules of hygiene. From a psychodynamic viewpoint the protagonists in both stories suffer from a depressive basic conflict, compensated in Bukowski's work by a dependent, self-destructive, philobatic form of coping and in Roche's alter ego Helen by an impulsive, sexualized behaviour.

  5. Submaximal Riemann-Roch expected curves and symplectic packing.

    Directory of Open Access Journals (Sweden)

    Wioletta Syzdek


    Full Text Available We study Riemann-Roch expected curves on $mathbb{P}^1 imes mathbb{P}^1$ in the context of the Nagata-Biran conjecture. This conjecture predicts that for sufficiently large number of points multiple points Seshadri constants of an ample line bundle on algebraic surface are maximal. Biran gives an effective lower bound $N_0$. We construct examples verifying to the effect that the assertions of the Nagata-Biran conjecture can not hold for small number of points. We discuss cases where our construction fails. We observe also that there exists a strong relation between Riemann-Roch expected curves on $mathbb{P}^1 imes mathbb{P}^1$ and the symplectic packing problem. Biran relates the packing problem to the existence of solutions of certain Diophantine equations. We construct such solutions for any ample line bundle on $mathbb{P}^1 imes mathbb{P}^1$ and a relatively smallnumber of points. The solutions geometrically correspond to Riemann-Roch expected curves. Finally we discuss in how far the Biran number $N_0$ is optimal in the case of mathbb{P}^1 imes mathbb{P}^1. In fact we conjecture that it can be replaced by a lower number and we provide evidence justifying this conjecture.

  6. Roche Accretion of stars close to massive black holes

    CERN Document Server

    Lixin,; Blandford, Roger D


    In this paper we consider Roche accretion in an Extreme Mass-Ratio Inspiral (EMRI) binary system formed by a star orbiting a massive black hole. The ultimate goal is to detect the mass and spin of the black hole and provide a test of general relativity in the strong-field regime from the resultant quasi-periodic signals. Before accretion starts, the stellar orbit is presumed to be circular and equatorial, and shrinks due to gravitational radiation. New fitting formulae are presented for the inspiral time and the radiation-reaction torque in the relativistic regime. If the inspiralling star fills its Roche lobe outside the Innermost Stable Circular Orbit (ISCO) of the hole, gas will flow through the inner Lagrange point (L1) to the hole. We give new relativistic interpolation formulae for the volume enclosed by the Roche lobe. If this mass-transfer happens on a time scale faster than the thermal time scale but slower than the dynamical time scale, the star will evolve adiabatically, and, in most cases, will re...

  7. Mécanique des sols et des roches

    CERN Document Server

    Vullier, Laurent; Zhao, Jian


    La mécanique des sols et la mécanique des roches sont des disciplines généralement traitées séparément dans la littérature. Pour la première fois, un traité réunit ces deux spécialités, en intégrant également les connaissances en lien avec les écoulements souterrains et les transferts thermiques. A la fois théorique et pratique, cet ouvrage propose tout d'abord une description détaillée de la nature et de la composition des sols et des roches, puis s'attache à la modélisation de problèmes aux conditions limites et présente les essais permettant de caractériser les sols et les roches, tant d'un point de vue mécanique qu'hydraulique et thermique. La problématique des sols non saturés et des écoulements multiphasiques est également abordée. Une attention particulière est portée aux lois de comportement mécanique et à la détermination de leurs paramètres par des essais in situ et en laboratoire, et l'ouvrage offre également une présentation détaillée des systèmes de classi...

  8. Comparison of real-time polymerase chain reaction with the COBAS Amplicor test for quantitation of hepatitis B virus DNA in serum samples

    Institute of Scientific and Technical Information of China (English)

    Ming Shi; Yong Zhang; Ying-Hua Zhu; Jing Zhang; Wei-Jia Xu


    AIM: To compare the clinical performance of a real-time PCR assay with the COBAS Amplicor Hepatitis B Virus (HBV) Monitor test for quantitation of HBV DNA in serum samples. METHODS: The reference sera of the Chinese National Institute for the Control of Pharmaceutical and Biological Products and the National Center for Clinical Laboratories of China, and 158 clinical serum samples were used in this study. The linearity, accuracy, reproducibility, assay time, and costs of the real-time PCR were evaluated and compared with those of the Cobas Amplicor test. RESULTS: The intra-assay and inter-assay variations of the real-time PCR ranged from 0.3% to 3.8% and 1.4% to 8.1%, respectively. The HBV DNA levels measured by the real-time PCR correlated very well with those obtained with the COBAS Amplicor test (r = 0.948). The real-time PCR HBV DNA kit was much cheaper and had a wider dynamic range. CONCLUSION: The real-time PCR assay is an excellent tool for monitoring of HBV DNA levels in patients with chronic hepatitis B.

  9. Roche volume filling of star clusters in the Milky Way (United States)

    Ernst, A.; Just, A.


    We examine the ratios rh/rJ of projected half-mass and Jacobi radius as well as rt/rJ of tidal and Jacobi radius for open and globular clusters in the Milky Way using data of both observations and simulations. We applied an improved calculation of rJ for eccentric orbits of globular clusters. A sample of 236 open clusters of Piskunov et al. within the nearest kiloparsec around the Sun has been used. For the Milky Way globular clusters, data are taken from the Harris catalogue. We particularly use the subsample of 38 Milky Way globular clusters for which orbits have been integrated by Dinescu et al. We aim to quantify the differences between open and globular clusters and to understand, why they form two intrinsically distinct populations. We find under certain assumptions, or, in other words, in certain approximations (i) that globular clusters are presently Roche volume underfilling, (ii) with at least 3σ confidence that the ratio rh/rJ of half-mass and Jacobi radius is three to five times larger at present for an average open cluster in our sample than for an average globular cluster in our sample and (iii) that a significant fraction of globular clusters may be Roche volume overfilling at pericentre with rt > rJ. Another aim of this paper is to throw light on the underlying theoretical reason for the existence of the van den Bergh correlation between half-mass and Galactocentric radius.

  10. Hot-Jupiter Core Mass from Roche-lobe Overflow

    CERN Document Server

    Ginzburg, Sivan


    The orbits of many observed hot Jupiters are decaying rapidly due to tidal interaction, eventually reaching the Roche limit. We analytically study the ensuing coupled mass loss and orbital evolution during the Roche-lobe overflow and find two possible scenarios. Planets with light cores $M_c\\lesssim 6M_\\oplus$ (assuming a nominal tidal dissipation factor $Q\\sim 10^6$ for the host star) are transformed into Neptune-mass gas planets, orbiting at a separation (relative to the stellar radius) $a/R_\\star\\approx 3.5$. Planets with heavier cores $M_c\\gtrsim 6M_\\oplus$ plunge rapidly until they are destroyed at the stellar surface. Remnant gas-Neptunes, which are stable to photo-evaporation, are absent from the observations, despite their unique transit radius ($5-10R_\\oplus$). This result suggests that $M_c\\gtrsim 6M_\\oplus$, providing a useful constraint on the poorly-known core mass that may distinguish between different formation theories of gas giants. Alternatively, given a prior estimate of $M_c\\approx 6 M_\\op...

  11. Comparison of two commercial assays for detection of human papillomavirus (HPV) in cervical scrape specimens: validation of the Roche AMPLICOR HPV test as a means to screen for HPV genotypes associated with a higher risk of cervical disorders.

    NARCIS (Netherlands)

    Ham, M.A. van; Bakkers, J.M.J.E.; Harbers, G.; Quint, W.G.V.; Massuger, L.F.A.G.; Melchers, W.J.G.


    Certain high-risk (HR) human papillomavirus (HPV) types are a necessary cause for the development of cervical disorders. Women with persistent HR HPV infections have an increased risk of developing high-grade cervical lesions, compared with those who have no or low-risk HPV infections. Therefore, im

  12. Small-scale hydro-electric scheme in Roches, Switzerland; Projet de concession. Petite centrale hydroelectrique de Roches

    Energy Technology Data Exchange (ETDEWEB)

    Hausmann, M.


    The small hydropower plant (SHPP) project 'PCH Roches' is planned to be built in Roches, a small village located in the Jura region of the canton of Bern. The goal of this undertaking is to reactivate a site with hydro potential of the river Birs on a section that was already well exploited by some ancient SHPP (since 1953 or probably even earlier). Several modifications were performed on this plant over the years; not all contributed positively on its production figures. Following a transfer of the water rights in 1987, claims became loud against the owner to perform maintenance on the river banks and to modify the weir such that a free passage for the fishes will be granted. Those issues contributed to inflate a conflict which ended in the repeal of the water rights and a total plant shut down in 2001. The new project plans to reuse the existing pen stock and to carry out some refurbishing activities to grant security and better performance. The weir and the intake structure are also redesigned such that the water storage can be somewhat raised for normal operation; a weir gate will however allow to better discharge the river in flood situations. A new turbine house will be erected at the current location where the tail water pen stock rejoins the river. Hence, the existing old turbine house will be obsolete and the upper and lower pen stock sections are to be linked with each other. Limited by the existing pen stock size, the new plant is expected to produce some 50 kW power and 362,000 kWh/year. This represents an increase in energy generation of around 200% compared with the ancient SHPP. Gross head is 5.38 m, net head 4.12 m with a turbine flow of at least 1.6 m{sup 3}/s. The expected energy sales price applicable to this project reaches about 0.17 CHF/kWh as per the current tariffs set by the grid owner. This project calls for the building of a state-of-the-art SHPP. It will avoid the total dismantling of the existing works to take place and allow

  13. Roche volume filling of star clusters in the Milky Way

    CERN Document Server

    Ernst, A


    We examine the ratios $r_h/r_J$ of projected half-mass and Jacobi radius as well as $r_t/r_J$ of tidal and Jacobi radius for open and globular clusters in the Milky Way using data of both observations and simulations. We applied an improved calculation of $r_J$ for eccentric orbits of globular clusters. A sample of 236 open clusters of Piskunov et al. within the nearest kiloparsec around the Sun has been used. For the Milky Way globular clusters, data are taken from the Harris catalogue. We particularly use the subsample of 38 Milky Way globular clusters for which orbits have been integrated by Dinescu et al. We aim to quantify the differences between open and globular clusters and to understand, why they form two intrinsically distinct populations. We find under certain assumptions, or, in other words, in certain approximations, (i) that globular clusters are presently Roche volume underfilling and (ii) with at least $3\\sigma$ confidence that the ratio $r_h/r_J$ of half-mass and Jacobi radius is $3 - 5$ time...

  14. Tidal Decay and Roche-Lobe Overflow of Gaseous Exoplanets (United States)

    Jackson, Brian; Jensen, Emily; Peacock, Sarah; Arras, Phil; Penev, Kaloyan


    Many gaseous exoplanets in short-period orbits are on the verge or are in the process of Roche-lobe overflow (RLO). Moreover, orbital stability analysis shows tides can drive many hot Jupiters to spiral inevitably toward their host stars, and the distributions of orbital periods and semi-major axes point to non-negligible orbital decay during the main sequence lifetimes of the host stars. Thus, the coupled processes of orbital evolution and RLO likely shape the observed distribution of close-in exoplanets. However, the exact outcome for an overflowing planet depends on its internal response to mass loss, and the accompanying orbital evolution can act to enhance or inhibit RLO. Applying the Modules for Experiments in Stellar Astrophysics (MESA) suite to model RLO, we find that, although the detailed evolution may depend on several properties of the planetary system, it is largely determined by the core mass of the overflowing gas giant. In particular, we find that the orbital expansion that accompanies RLO often stops and reverses at a specific maximum period that depends on the core mass. We suggest that RLO may often strand the remnant of a gas giant near or interior to this orbital period, providing an observational prediction that can corroborate the RLO hypothesis. We conduct a preliminary comparison of this prediction to the observed population of small, short-period planets and find some planets in orbits that may be consistent with this picture.

  15. Disainmööbel Roche Bobois, kokteil sarmist ja romantikast / Reet Krause

    Index Scriptorium Estoniae

    Krause, Reet, 1967-


    Luksuslikku kodumööblit tootvast prantsuse firmast. Firma disainerid itaallased Paola Navone ja Massimo Iosa Ghini, Vladimir Kagan, Hans Hopper ja prantslased Jean Claude Magirard ja Sylvain Joly. Philippe Roche ja disainer Hans Hopperi kommentaar

  16. Unstable Roche-Lobe Overflow of Gaseous Planets (United States)

    Jackson, Brian

    The discoveries of more than 100 roughly Earth-sized bodies with orbital periods less than 1 day, ultra-short-period planets or candidates (USPs), have challenged planet formation theories, and evidence suggests USPs may be the remnants of gaseous planets that shed their atmospheres. Indeed, many hot Jupiters are near Roche-Lobe overflow (RLO), and tidal decay can push them the rest of the way in. Recent work has shown stable RLO (atmospheres lost via a steady outflow and thin accretion disk) probably cannot produce USPs on its own but suggested unstable RLO (atmospheres quickly shed on dynamical timescales) may. In fact, stable RLO may drive overflowing hot Jupiters into unstable RLO, and by analogy with the common-envelope binaries, the core that remains can drive off the gaseous envelope at the cost of its orbital energy. Wellestablished mass-radius relations for gaseous planets, coupled to simple energy and angular momentum considerations, provide a connection between the observed masses and periods for USPs and their putative progenitor gaseous planets, with few free parameters. We propose to investigate the hypothesis that USPs originate through tidal decay and a combination of stable and unstable Roche-lobe overflow of short-period gaseous planets through the following studies: -We will explore the planetary masses, orbital periods, etc. that produce unstable RLO using the Modules for Experiments in Stellar Astrophysics (MESA) suite. -We will relate the observed periods and masses of USPs to their putative progenitor masses and periods to see whether they are consistent with the unstable RLO hypothesis. This proposal is directly relevant to the Exoplanets Research Program since it seeks to "understand the ... physical processes of exoplanets" and "improve understanding of [their] origins" through "theoretical studies ... and modeling'". We also expect that it will have broad impacts on a variety of astrophysical topics: -Ultra-short period planets could

  17. Analytical validation of the Roche 25-OH Vitamin D Total assay

    DEFF Research Database (Denmark)

    Knudsen, Cindy Soendersoe; Nexo, Ebba; Højskov, Carsten Schriver;


    Background: Vitamin D deficiency is considered a major health issue and therefore there is a need for reliable routine tests for measurement of the vitamin in blood samples. Here we present a validation of the recently released Roche 25-OH Vitamin D Total assay (Vitamin D Total). Methods: We anal...

  18. 75 FR 71135 - Hoffmann-La Roche Inc.; Withdrawal of Approval of a New Drug Application (United States)


    ... HUMAN SERVICES Food and Drug Administration Hoffmann-La Roche Inc.; Withdrawal of Approval of a New Drug... Administration (FDA) is withdrawing approval of a new drug application (NDA) for ACCUTANE (isotretinoin) Capsules... be marketed under approved abbreviated new drug applications (ANDAs). The holders of ANDAs...

  19. Performance of the Roche LightCycler real-time PCR assay for diagnosing extrapulmonary tuberculosis. (United States)

    Gous, N; Scott, L E; Wong, E; Omar, T; Venter, W D F; Stevens, W


    The Roche LightCycler mycobacterium detection molecular assay for Mycobacterium tuberculosis, M. avium, and M. kansasii, was applied to tissue specimens. It performed well on lymph node and cerebrospinal fluid specimens and less well on lung, liver, and bone marrow core biopsy specimens, but used in conjunction with a clinical suspicion of tuberculosis, it could augment patient management.

  20. On the Use of Roche Equipotentials in Analysing the Problems of Binary and Rotating Stars

    Indian Academy of Sciences (India)

    A. Pathania; A. K. Lal; C. Mohan


    Kopal (Adv. Astron. Astrophys., 9, 1, 1972) introduced the concept of Roche equipotentials to analyse the effects of rotational and tidal distortions in case of stars in binary systems. In this approach a mathematical expression for the potential of a star in a binary system is obtained by approximating its inner structure with Roche model. This expression for the potential has been used in subsequent analysis by various authors to analyse the problems of structures and oscillations of synchronous and nonsynchronus binary stars as well as single rotating stars. Occasionally, doubts have been expressed regarding the validity of the use of this approach for analysing nonsynchronous binaries and rotationally and tidally distorted single stars. In this paper we have tried to clarify these doubts.

  1. Thermal waters as cosmeceuticals: La Roche-Posay thermal spring water example

    Directory of Open Access Journals (Sweden)

    Seite S


    Full Text Available Sophie SeiteLa Roche-Posay Pharmaceutical Laboratories, Asnières, FranceAbstract: The curative use of thermal spring water is well known, but further investigation of its biological properties and therapeutic benefits is necessary. This present article reports all available scientific data concerning La Roche-Posay Thermal Spring Water and provides a better understanding of the biological mechanism of action of this water in regard to its composition and physicochemical properties and its clinical benefits for patients. These data justify the use of this selenium-rich water as an active or “cosmeceutical” ingredient in topical formulations to increase quality of life and compliance in patients with chronic disease.Keywords: thermal spring water, selenium, biological properties, curative use

  2. Roche DAT immunoassay: sensitivity and specificity testing for amphetamines, cocaine, and opiates in oral fluid. (United States)

    Crooks, C Richard; Brown, Sue


    Laboratory testing of oral fluid for drugs of abuse continues to expand in the workplace, legal, treatment, and health settings. In this study, we assessed recently developed homogeneous Roche DAT screening assays for amphetamines, cocaine metabolite [benzoylecgonine (BZE)], methamphetamines, and opiates in oral fluid. Precision and accuracy were assessed using control samples at +/-25% of cutoff. Sensitivity, specificity, and agreement compared to liquid chromatography-tandem mass spectrometry (LC-MS-MS) was assessed by analysis of oral fluid specimens collected from 994 subjects enrolled in a drug treatment or probation and parole drug-testing program. An additional 180 research specimens from Kroll Laboratories were analyzed for amphetamine and methamphetamine. Screening cutoff concentrations (ng/mL) were as follows: amphetamines, 40; cocaine metabolite, 3; methamphetamines, 40; and opiates, 10. LC-MS-MS analyses were performed with the following cutoff concentrations (ng/mL): amphetamine, 40; BZE, 2.0; methamphetamine, 40; and codeine or morphine, 10. The percent coefficient of variation ranged from 3.4% to 7.3%. Sensitivity and specificity of the Roche DAT assays compared to LC-MS-MS were > 94%, and agreement was > 96% for the four assays. The performance of the Roche DAT assays suggests these new homogeneous screening assays will be an attractive alternative to existing more labor-intensive enzyme immunoassays.

  3. Tidally-driven Roche-Lobe Overflow of Hot Jupiters with MESA

    CERN Document Server

    Valsecchi, Francesca; Rasio, Frederic A; Marchant, Pablo; Rogers, Leslie A


    Many exoplanets have now been detected in orbits with ultra-short periods, very close to the Roche limit. Building upon our previous work, we study the possibility that mass loss through Roche lobe overflow (RLO) may affect the evolution of these planets, and could possibly transform a hot Jupiter into a lower-mass planet (hot Neptune or super-Earth). We focus here on systems in which the mass loss occurs slowly ("stable mass transfer" in the language of binary star evolution) and we compute their evolution in detail with the binary evolution code MESA. We include the effects of tides, RLO, irradiation and photo-evaporation of the planet, as well as the stellar wind and magnetic braking. Our calculations all start with a hot Jupiter close to its Roche limit, in orbit around a sun-like star. The initial orbital decay and onset of RLO are driven by tidal dissipation in the star. We confirm that such a system can indeed evolve to produce lower-mass planets in orbits of a few days. The RLO phase eventually ends a...

  4. From Hot Jupiters to Super-Earths via Roche Lobe Overflow

    CERN Document Server

    Valsecchi, Francesca; Steffen, Jason H


    Through tidal dissipation in a slowly spinning host star the orbits of many hot Jupiters may decay down to the Roche limit. We expect that in most cases the ensuing mass transfer will be stable. Using detailed numerical calculations we find that this evolution is quite rapid, potentially leading to complete removal of the gaseous envelope in a few Gyr, and leaving behind an exposed rocky core ("hot super-Earth"). Final orbital periods are quite sensitive to the details of the planet's mass-radius relation, and to the effects of irradiation and photo-evaporation, but could be as short as a few hours, or as long as several days. Our scenario predicts the existence of planets with intermediate masses ("hot Neptunes") that should be found precisely at their Roche limit and in the process of losing mass through Roche lobe overflow. The observed excess of small single-planet candidate systems observed by Kepler may also be the result of this process. If so, the properties of their host stars should track those of t...

  5. Galaxies with Supermassive Binary Black Holes: (III) The Roche Lobes and Jiang-Yeh Lobe in a Core System

    CERN Document Server

    Yeh, Li-Chin


    Three-dimensional equi-potential surfaces of a galactic system with supermassive binary black holes are discussed herein. The conditions of topological transitions for the important surfaces, i.e. Roche Lobes and Jiang-Yeh Lobe, are studied in this paper. In addition, the mathematical properties of the Jacobi surfaces are investigated analytically. Finally, a numerical procedure for determining the regions of the Roche Lobes and Jiang-Yeh Lobe is suggested.


    Energy Technology Data Exchange (ETDEWEB)

    Valsecchi, Francesca; Rasio, Frederic A. [Center for Interdisciplinary Exploration and Research in Astrophysics (CIERA), and Northwestern University, Department of Physics and Astronomy, Evanston, IL 60208 (United States); Rappaport, Saul [Department of Physics, and Kavli Institute for Astrophysics and Space Research, Massachusetts Institute of Technology, Cambridge, MA 02139 (United States); Marchant, Pablo [Argelander-Institut für Astronomie, Universität Bonn, Auf dem Hgel 71, D-53121 Bonn (Germany); Rogers, Leslie A., E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [Department of Astronomy and Department of Geophysics and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125 (United States)


    Many exoplanets have now been detected in orbits with ultra-short periods very close to the Roche limit. Building upon our previous work, we study the possibility that mass loss through Roche lobe overflow (RLO) may affect the evolution of these planets, and could possibly transform a hot Jupiter into a lower-mass planet (hot Neptune or super-Earth). We focus here on systems in which the mass loss occurs slowly (“stable mass transfer” in the language of binary star evolution) and we compute their evolution in detail with the binary evolution code Modules for Experiments in Stellar Astrophysics. We include the effects of tides, RLO, irradiation, and photo-evaporation (PE) of the planet, as well as the stellar wind and magnetic braking. Our calculations all start with a hot Jupiter close to its Roche limit, in orbit around a Sun-like star. The initial orbital decay and onset of RLO are driven by tidal dissipation in the star. We confirm that such a system can indeed evolve to produce lower-mass planets in orbits of a few days. The RLO phase eventually ends and, depending on the details of the mass transfer and on the planetary core mass, the orbital period can remain around a few days for several Gyr. The remnant planets have rocky cores and some amount of envelope material, which is slowly removed via PE at a nearly constant orbital period; these have properties resembling many of the observed super-Earths and sub-Neptunes. For these remnant planets, we also predict an anti-correlation between mass and orbital period; very low-mass planets (M{sub pl} ≲ 5 M{sub ⊕}) in ultra-short periods (P{sub orb} < 1 day) cannot be produced through this type of evolution.

  7. Tidally-driven Roche-lobe Overflow of Hot Jupiters with MESA (United States)

    Valsecchi, Francesca; Rappaport, Saul; Rasio, Frederic A.; Marchant, Pablo; Rogers, Leslie A.


    Many exoplanets have now been detected in orbits with ultra-short periods very close to the Roche limit. Building upon our previous work, we study the possibility that mass loss through Roche lobe overflow (RLO) may affect the evolution of these planets, and could possibly transform a hot Jupiter into a lower-mass planet (hot Neptune or super-Earth). We focus here on systems in which the mass loss occurs slowly (“stable mass transfer” in the language of binary star evolution) and we compute their evolution in detail with the binary evolution code Modules for Experiments in Stellar Astrophysics. We include the effects of tides, RLO, irradiation, and photo-evaporation (PE) of the planet, as well as the stellar wind and magnetic braking. Our calculations all start with a hot Jupiter close to its Roche limit, in orbit around a Sun-like star. The initial orbital decay and onset of RLO are driven by tidal dissipation in the star. We confirm that such a system can indeed evolve to produce lower-mass planets in orbits of a few days. The RLO phase eventually ends and, depending on the details of the mass transfer and on the planetary core mass, the orbital period can remain around a few days for several Gyr. The remnant planets have rocky cores and some amount of envelope material, which is slowly removed via PE at a nearly constant orbital period; these have properties resembling many of the observed super-Earths and sub-Neptunes. For these remnant planets, we also predict an anti-correlation between mass and orbital period; very low-mass planets (Mpl ≲ 5 M⊕) in ultra-short periods (Porb < 1 day) cannot be produced through this type of evolution.

  8. Exo-Mercury Analogues and the Roche Limit for Close-Orbiting Rocky Planets (United States)

    Rogers, Leslie A.; Price, Ellen


    The origin of Mercury's enhanced iron content is a matter of ongoing debate. The characterization of rocky exoplanets promises to provide new independent insights on this topic, by constraining the occurrence rate and physical and orbital properties of iron-enhanced planets orbiting distant stars. The ultra-short-period transiting planet candidate KOI-1843.03 (0.6 Earth-radius, 4.245 hour orbital period, 0.46 Solar-mass host star) represents the first exo-Mercury planet candidate ever identified. For KOI-1843.03 to have avoided tidal disruption on such a short orbit, Rappaport et al. (2013) estimate that it must have a mean density of at least 7g/cc and be at least as iron rich as Mercury. This density lower-limit, however, relies upon interpolating the Roche limits of single-component polytrope models, which do not accurately capture the density profiles of >1000 km differentiated rocky bodies. A more exact calculation of the Roche limit for the case of rocky planets of arbitrary composition and central concentration is needed. We present 3D interior structure simulations of ultra-short-period tidally distorted rocky exoplanets, calculated using a modified version of Hachisu’s self-consistent field method and realistic equations of state for silicates and iron. We derive the Roche limits of rocky planets as a function of mass and composition, and refine the composition constraints on KOI-1843.03. We conclude by discussing the implications of our simulations for the eventual characterization of short-period transiting planets discovered by K2, TESS, CHEOPS and PLATO.

  9. Self-sustained strong mass transfer without Roche lobe overflow - Cygnus X-3 (United States)

    Tavani, Marco; Ruderman, Malvin; Shaham, Jacob


    It is proposed that the binary evolution of Cyg X-3 is driven by a self-excited wind from a solar composition companion star sustained by radiation from a neutron star primary. Observations would then imply that the companion is a white dwarf underfilling its Roche lobe, with mass between 0.01 and 0.03 solar masses. Cyg X-3 could then be in the late stage of very low mass X-ray binary evolution expected to result in a millisecond pulsar binary similar to the eclipsing system PSR 1957 + 20.

  10. Teorema de Riemann-Roch, morfismos de Frobenius e a hipótese de Riemann



    The aim of this work is to estimate a bound for the number of rational points of a curve. Observing the various similarities between the ring of integers and the ring of polynomials in one variable, we use tools from number theory to solve a problem of algebraic geometry. From this merger is born one of the noblest areas of mathematics: arithmetic geometry. Making use of the famous Riemann-Roch's theorem and tools of number theory we demonstrate the Riemann hypothesis for the zeta-function of...

  11. A New Model of Roche Lobe Overflow for Short-period Gaseous Planets and Binary Stars (United States)

    Jackson, Brian; Arras, Phil; Penev, Kaloyan; Peacock, Sarah; Marchant, Pablo


    Some close-in gaseous exoplanets are nearly in Roche lobe contact, and previous studies show that tidal decay can drive hot Jupiters into contact during the main sequence of their host stars. Improving on a previous model, we present a revised model for mass transfer in a semidetached binary system that incorporates an extended atmosphere around the donor and allows for an arbitrary mass ratio. We apply this new formalism to hypothetical, confirmed, and candidate planetary systems to estimate mass-loss rates and compare with models of evaporative mass loss. Overflow may be significant for hot Neptunes out to periods of ∼2 days, while for hot Jupiters, it may only be important inward of 0.5 days. We find that CoRoT-24 b may be losing mass at a rate of more than an Earth mass in a gigayear. The hot Jupiter WASP-12 b may lose an Earth mass in a megayear, while the putative planet PTFO8-8695 orbiting a T Tauri star might shed its atmosphere in a few megayears. We point out that the orbital expansion that can accompany mass transfer may be less effective than previously considered because the gas accreted by the host star removes some of the angular momentum from the orbit, but simple scaling arguments suggest that the Roche lobe overflow might remain stable. Consequently, the recently discovered small planets in ultrashort periods (model presented here has been incorporated into Modules for Experiments in Stellar Astrophysics (MESA).

  12. Wind Roche-lobe overflow: Application to carbon-enhanced metal-poor stars

    CERN Document Server

    Abate, C; Izzard, R G; Mohamed, S S; de Mink, S E


    Carbon-enhanced metal-poor stars (CEMP) are observed as a substantial fraction of the very metal-poor stars in the Galactic halo. Most CEMP stars are also enriched in s-process elements and these are often found in binary systems. This suggests that the carbon enrichment is due to mass transfer in the past from an asymptotic giant branch (AGB) star on to a low-mass companion. Models of binary population synthesis are not able to reproduce the observed fraction of CEMP stars without invoking non-standard nucleosynthesis or a substantial change in the initial mass function. This is interpreted as evidence of missing physical ingredients in the models. Recent hydrodynamical simulations show that efficient wind mass transfer is possible in the case of the slow and dense winds typical of AGB stars through a mechanism called wind Roche-lobe overflow (WRLOF), which lies in between the canonical Bondi-Hoyle-Lyttleton (BHL) accretion and Roche-lobe overflow. WRLOF has an effect on the accretion efficiency of mass tran...

  13. iAssembler: a package for de novo assembly of Roche-454/Sanger transcriptome sequences

    Directory of Open Access Journals (Sweden)

    Zheng Yi


    Full Text Available Abstract Background Expressed Sequence Tags (ESTs have played significant roles in gene discovery and gene functional analysis, especially for non-model organisms. For organisms with no full genome sequences available, ESTs are normally assembled into longer consensus sequences for further downstream analysis. However current de novo EST assembly programs often generate large number of assembly errors that will negatively affect the downstream analysis. In order to generate more accurate consensus sequences from ESTs, tools are needed to reduce or eliminate errors from de novo assemblies. Results We present iAssembler, a pipeline that can assemble large-scale ESTs into consensus sequences with significantly higher accuracy than current existing assemblers. iAssembler employs MIRA and CAP3 assemblers to generate initial assemblies, followed by identifying and correcting two common types of transcriptome assembly errors: 1 ESTs from different transcripts (mainly alternatively spliced transcripts or paralogs are incorrectly assembled into same contigs; and 2 ESTs from same transcripts fail to be assembled together. iAssembler can be used to assemble ESTs generated using the traditional Sanger method and/or the Roche-454 massive parallel pyrosequencing technology. Conclusion We compared performances of iAssembler and several other de novo EST assembly programs using both Roche-454 and Sanger EST datasets. It demonstrated that iAssembler generated significantly more accurate consensus sequences than other assembly programs.

  14. Racialized Spaces in Teacher Discourse: A Critical Discourse Analysis of Place-Based Identities in Roche Bois, Mauritius (United States)

    Wiehe, Elsa M.


    This eleven-month ethnographic study puts critical discourse analysis in dialogue with postmodern conceptualizations of space and place to explore how eight educators talk about space and in the process, produce racialized spaces in Roche Bois, Mauritius. The macro-historical context of racialization of this urban marginalized community informs…

  15. Correlación en la medición de la carga viral para VIH 1 entre las técnicas de PCR amplicor y PCR tiempo real



    El objetivo es determinar el nivel de correlación de la cuantificación de carga viral para VIH-1 mediante las técnicas Amplicor HIV-1 Monitor Versión Estándar y Sistema COBAS Ampliprep/COBAS Taqman 48 HIV-1; los materiales y métodos due que se realizó un estudio correlacional, se incluyó 152 muestras de plasma, de personas viviendo con VIH/SIDA que se encuentran en el Sistema de Monitoreo de TARGA del INS. Las muestras incluidas tenían carga viral en rango de 400 a 750,000 copias/mL, y se a...

  16. The evolution of triples with a Roche-lobe filling outer star

    CERN Document Server

    de Vries, Nathan; Figueira, Joana


    The evolution of triples has not attracted much attention in the literature, although their evolution can be dramatically different from binaries and single stars. Triples are quite common, and we find that for about 1% of the triples in the Tokovinin catalogue of multiple stellar systems in the solar neighbourhood, the tertiary star will overflow its Roche lobe at some time in its evolution, before any of the inner stars leave the main sequence. For two of these systems, Xi Tauri and HD97131 we simulate in detail this phase of mass transfer, during which stellar evolution, gravitational dynamics and hydrodynamics all play an important role. We have used the Astrophysical Multi-purpose Software Environment (AMUSE) to solve these physical processes in a self-consistent way. The resulting evolution, mass transfer and the effects on the inner as well as on the outer orbit are profound, although it is not trivial to predict the eventual consequence of the phase of mass transfer and the appearance of the resulting...

  17. Roche tomography of cataclysmic variables - VII. The long-term magnetic activity of AE Aqr

    CERN Document Server

    Hill, C A; Steeghs, D; Dhillon, V S; Shahbaz, T


    We present a long-term study of the secondary star in the cataclysmic variable AE~Aqr, using Roche tomography to indirectly image starspots on the stellar surface spanning 8~years of observations. The 7 maps show an abundance of spot features at both high and low latitudes. We find that all maps have at least one large high-latitude spot region, and we discuss its complex evolution between maps, as well as its compatibility with current dynamo theories. Furthermore, we see the apparent growth in fractional spot coverage, $f_{\\mathrm{s}}$, around $45^{\\circ}$~latitude over the duration of observations, with a persistently high $f_{\\mathrm{s}}$ near latitudes of $20^{\\circ}$. These bands of spots may form as part of a magnetic activity cycle, with magnetic flux tubes emerging at different latitudes, similar to the `butterfly' diagram for the Sun. We discuss the nature of flux tube emergence in close binaries, as well as the activity of AE~Aqr in the context of other stars.

  18. Structures in the D ring and Roche division tied to asymmetries in Saturn's magnetosphere (United States)

    Chancia, Robert O.; Hedman, Matthew M.; Carter, Brian


    Saturn's dusty rings contain multiple patterns that appear to be rotating around the planet at about the same rate as Saturn itself, and so are probably generated by resonances with asymmetries in the planet's gravitational or magnetic fields. These structures are found in the D ring (which lies interior to the main rings) and in the Roche Division (which is located just outside the main rings, between the A and F rings). In 2007 the strongest patterns in both of these regions appeared to track magnetospheric anomalies associated with the Saturn Kilometric Radiation (SKR). This implied that these rings were responding to structures in the planet's magnetosphere, which is not unreasonable since the particles in both these ring regions are very small and therefore sensitive to non-gravitational forces. Over the last few years, the frequencies of the SKR and related magnetospheric asymmetries have shifted, and we have observed some changes in the ring patterns that might be connected with these shifts. However, there are also patterns in these rings that appear to have more stable rotation rates and so could reflect more persistent asymmetries in Saturn's magnetosphere. These patterns can therefore provide novel insights into the structure and evolution of Saturn's magnetosphere.

  19. The Nother and Riemann-Roch type theorems for piecewise algebraic curve

    Institute of Scientific and Technical Information of China (English)


    A piecewise algebraic curve is a curve determined by the zero set of a bivariate spline function. In this paper, the Nother type theorems for Cμpiecewise algebraic curves are obtained. The theory of the linear series of sets of places on the piecewise algebraic curve is also established. In this theory, singular cycles are put into the linear series, and a complete series of the piecewise algebraic curves consists of all effective ordinary cycles in an equivalence class and all effective singular cycles which are equivalent specifically to any effective ordinary cycle in the equivalence class. This theory is a generalization of that of linear series of the algebraic curve. With this theory and the fundamental theory of multivariate splines on smoothing cofactors and global conformality conditions, and the results on the general expression of multivariate splines, we get a formula on the index, the order and the dimension of a complete series of the irreducible Cμpiecewise algebraic curves and the degree, the genus and the smoothness of the curves, hence the Riemann-Roch type theorem of the Cμpiecewise algebraic curve is established.

  20. Evaluation of the Roche CoaguChek XS handheld coagulation analyzer in a cardiac outpatient clinic. (United States)

    Nam, Myung-Hyun; Roh, Kyoung Ho; Pak, Hui-Nam; Lee, Chang Kyu; Kim, Young-Hoon; Lee, Kap No; Cho, Yunjung


    This study evaluated the performance of a handheld coagulation analyzer for measurements of capillary blood specimens of 93 outpatient cardiology patients with atrial fibrillation who were receiving oral anti-coagulant therapy. The international normalized ratio (INR) results of the CoaguChek XS system (Roche Diagnostics) were compared with those obtained in the central laboratory with citrated venous blood specimens using the ACL9000 coagulation analyzer (Instrumentation Laboratory). The INR results for prothrombin time by the CoaguChek XS analyzer were closely correlated with the central laboratory's results in the INR range of 0.96 approximately 8.53 (r = 0.964). A statistically significant difference was noted between 2 lots of test strips, but the difference was miniscule (mean +/- 95% confidence interval: 0.04+/-0.02). The CV of 8 replicate assays with the CoaguChek XS for a blood specimen with high INR value (INR=3.9) was 1.4%; for a blood specimen with medium INR value (INR=1.3), the CV of 8 replicate assays was XS analyzer is precise and reliable for assessment of INR results at clinically significant ranges in cardiac outpatients.

  1. The N(o)ther and Riemann-Roch type theorems for piecewise algebraic curve

    Institute of Scientific and Technical Information of China (English)

    Yi-sheng LAI; Ren-hong WANG


    A piecewise algebraic curve is a curve determined by the zero set of a bivariate spline function. In this paper, the N(o)ther type theorems for Cμ piecewise algebraic curves are obtained.The theory of the linear series of sets of places on the piecewise algebraic curve is also established.In this theory, singular cycles are put into the linear series, and a complete series of the piecewise algebraic curves consists of all effective ordinary cycles in an equivalence class and all effective singular cycles which are equivalent specifically to any effective ordinary cycle in the equivalence class. This theory is a generalization of that of linear series of the algebraic curve. With this theory and the fundamental theory of multivariate splines on smoothing cofactors and global conformality conditions,and the results on the general expression of multivariate splines, we get a formula on the index, the order and the dimension of a complete series of the irreducible Cμ piecewise algebraic curves and the degree, the genus and the smoothness of the curves, hence the Riemann-Roch type theorem of the Cμpiecewise algebraic curve is established.

  2. Roche tomography of cataclysmic variables - VI. Differential rotation of AE Aqr - Not tidally locked!

    CERN Document Server

    Hill, Colin; Shahbaz, Tariq; Steeghs, Danny; Dhillon, Vik


    We present Roche tomograms of the K4V secondary star in the cataclysmic variable AE Aqr, reconstructed from two datasets taken 9 days apart, and measure the differential rotation of the stellar surface. The tomograms show many large, cool starspots, including a large high-latitude spot and a prominent appendage down the trailing hemisphere. We find two distinct bands of spots around 22$^{\\circ}$ and 43$^{\\circ}$ latitude, and estimate a spot coverage of 15.4-17% on the northern hemisphere. Assuming a solar-like differential rotation law, the differential rotation of AE Aqr was measured using two different techniques. The first method yields an equator-pole lap time of 269 d and the second yields a lap time of 262 d. This shows the star is not fully tidally locked, as was previously assumed for CVs, but has a co-rotation latitude of $\\sim 40^{\\circ}$. We discuss the implications that these observations have on stellar dynamo theory, as well as the impact that spot traversal across the first Lagrangian point ma...

  3. El concepto de límite en el B-Mu de François Roche

    Directory of Open Access Journals (Sweden)

    Eugenio Pandolfini


    Full Text Available


    Algunos proyectos, como el Dusty relief/B‐mu (2002 de François Roche demuestran como edificios complejos, que toman distancia desde los modelos mecanicistas para referirse a nuevos paradigmas, se pueden interpretar y comprenderse  mejor gracias a un análisis perceptivo que acerca el proyecto de arquitectura a cuestiones como la relación psicológica del hombre con la arquitectura, el miedo al espacio, y las  patologías  vinculadas a la percepción y a las neurosis modernas.
    En este caso, aparte de las repercusiones que la fachada de  polvo podría tener en el ámbito de la ecología urbana, es interesante analizar algunos aspectos ligados a la dicotomía  entre forma externa y volúmenes internos para la que François Roche cita como referencia el raumplan de Adolf Loos, pero que presenta motivos para una reflexión vinculada a los aspectos  perceptivos.
    El artículo trata de analizar como François Roche proyecta sus edificios extremando la dicotomía entre interior/visual y  exterior/táctil, desarrollando así una nueva relación con el lugar. Roche diseña la fachada exterior del B‐mu autoimponiéndose  una limitación del sentido de la vista, a favor de una dimensión háptica del proyecto y lo hace envolviendo los ámbitos arquitectónicos más familiares de una interfaz abstracta y táctil.

    Palabras clave

    edificio, percepción, entorno, envolvente, límite


    Some projects such as Dusty relief/B‐mu (2002 by François  Roche demonstrate how complex buildings, which distance themselves from the mechanicist models in order to refer  themselves to new paradigms, can be better understood and interpreted thanks to a perceptive analysis.
    This analysis brings the architectural project closer to matters  such as man’s psychological relationship to Architecture, the fear of space

  4. Polyphony and counterpoint: Mechanisms of seduction in the diaries of Helen Hessel and Henri Pierre Roché

    Directory of Open Access Journals (Sweden)

    Catherine du Toit


    Full Text Available Henri Pierre Roché (1879–1959, the author of Jules et Jim, has been called a general introducer, an exemplary amateur, a collector of women and art and one of the most prolific diarists and active lovers in recorded history. Author of a collection of vignettes about Don Juan, Roché was fascinated with the figure of the seducer and in his twenties planned to devote his life to the creation of a body of work which would examine moral, intellectual, social and sexual relations between women and men. To this end, he would transform his life into a laboratory where real-life experiences would become the main source of reference. Roché’s diary spans sixty years and abounds in tales of seduction. However, the most intense and captivating intrigue of seduction and betrayal in his diary, is his relationship with Helen Hessel. At the start of their affair, Roché suggested that she too should keep a diary of the maelstrom of passion into which they were plunged. Written in French, German and English, Helen Hessel’s diary captures the drama of seduction and functions on several levels: realistic, visionary, absorbed in her thoughts and emotions and yet critical of herself and others. A juxtaposed reading of the two diaries generates a fascinatingly dense texture, revealing the mechanisms of seduction at play. The counterpoint created by these two interdependent voices becomes ever more complex as one becomes aware of the intertextual references that contribute to the emerging polyphony of recorded life and love.Polifonie en kontrapunt: Meganismes van verleiding in die dagboeke van Helen Hessel en Henri Pierre Roché. Henri Pierre Roché (1879–1959, outeur van Jules et Jim, word beskryf as ‘n sosiale koppelaar, ‘n model-liefhebber van alles en nog wat, ‘n versamelaar van vroue en kuns en een van die mees produktiewe dagboekskrywers en aktiewe minnaars in die opgetekende geskiedenis. Roché het ‘n reeks sketse oor Don Juan gepubliseer en

  5. The Quasi-Roche lobe overflow state in the evolution of Close Binary Systems containing a radio pulsar

    CERN Document Server

    Benvenuto, O G; Horvath, J E


    We study the evolution of close binary systems formed by a normal (solar composition), intermediate mass donor star together with a neutron star. We consider models including irradiation feedback and evaporation. These non-standard ingredients deeply modify the mass transfer stages of these binaries. While models that neglect irradiation feedback undergo continuous, long standing mass transfer episodes, models including these effect suffer a number cycles of mass transfer and detachment. During mass transfer the systems should reveal themselves as low-mass X-ray binaries (LMXBs), whereas when detached they behave as a binary radio pulsars. We show that at these stages irradiated models are in a Roche lobe overflow (RLOF) state or in a quasi-RLOF state. Quasi-RLOF stars have a radius slightly smaller than its Roche lobe. Remarkably, these conditions are attained for orbital period and donor mass values in the range corresponding to a family of binary radio pulsars known as "redbacks". Thus, redback companions ...


    Institute of Scientific and Technical Information of China (English)



    The author presents an extension of the Atiyah-Patodi-Singer invariant for unitary representations [2, 3] to the non-unitary case, as well as to the case where the base manifold admits certain finer structures. In particular, when the base manifold has a fibration structure, a Riemann-Roch theorem for these invariants is established by computing the adiabatic limits of the associated η-invariants.

  7. QTrim : a novel tool for the quality trimming of sequence reads generated using the Roche/454 sequencing platform


    Shrestha, Ram; Lubinsky, Baruch; Bansode, Vijay B; Moinz, Mónica B. J.; McCormack, Grace P.; Travers, Simon A


    Background\\ud Many high throughput sequencing (HTS) approaches, such as the Roche/454 platform, produce sequences in which the quality of the sequence (as measured by a Phred-like quality scores) decreases linearly across a sequence read. Undertaking quality trimming of this data is essential to enable confidence in the results of subsequent downstream analysis. Here, we have developed a novel, highly sensitive and accurate approach (QTrim) for the quality trimming of sequence reads generated...

  8. Some physiological changes in Waldsteinia trifolia (Roch. leaves in different months of the year

    Directory of Open Access Journals (Sweden)

    Renata Wojciechowska


    Full Text Available The study concerned an evergreen herbal plant Waldsteinia trifolia Roch. This species can be found more and more often in garden plantings in Poland. The aim of the study was to estimate the content of photosynthetic pigments, anthocyanins and dry matter as well as the permeability of cytoplasmatic membranes of Waldsteinia leaves. Plants grew in an ornamental plant collection at the premises of the Faculty of Horticulture of the Agricultural University in Kraków. The study was conducted in 2006 and 2007 in the following months: March, May, June, September, October and November. The permeability of cytoplasmatic membranes was estimated based on electrolyte leakage from leaf discs at 20°C in relation to total electrolytes in the tissue after unfreezing. The first days with temperature drops down to around 0°C in autumn resulted in a decrease in chlorophyll a, b and carotenoid content, but significantly increased anthocyanin content. The ratio of chlorophyll a to b and the ratio of total chlorophylls to carotenoids did not change in particular months. In both years of study, similar changes in dry matter content of the Waldsteinia leaves were observed. Dry matter content significantly increased in June and November and decreased in May and October. In June and in autumn, after first slight frosts, an increase of electrolyte leakage from the leaf discs was observed. However, high dry matter content (40.3% was accompanied by lower permeability of the cytoplasmatic membranes of the leaves after winter during March. These results have shown good adaptation of Waldsteinia trifolia plants to the climatic conditions in Poland.

  9. Elevated ethyl methanesulfonate (EMS in nelfinavir mesylate (Viracept®, Roche: overview

    Directory of Open Access Journals (Sweden)

    Jones Judith K


    Full Text Available Abstract Roche's protease inhibitor nelfinavir mesylate (Viracept® produced between March 2007-June 2007 was found to contain elevated levels of ethyl methanesulfonate (EMS, a known mutagen (alkylator – leading to a global recall of the drug. EMS levels in a daily dose (2,500 mg Viracept/day were predicted not to exceed a dose of ~2.75 mg/day (~0.055 mg/kg/day based on 50 kg patient. As existing toxicology data on EMS did not permit an adequate patient risk assessment, a comprehensive animal toxicology evaluation of EMS was conducted. General toxicity of EMS was investigated in rats over 28 days. Two studies for DNA damage were performed in mice; chromosomal damage was assessed using a micronucleus assay and gene mutations were detected using the MutaMouse transgenic model. In addition, experiments designed to extrapolate animal exposure to humans were undertaken. A general toxicity study showed that the toxicity of EMS occurred only at doses ≥ 60 mg/kg/day, which is far above that received by patients. Studies for chromosomal damage and mutations in mice demonstrated a clear threshold effect with EMS at 25 mg/kg/day, under chronic dosing conditions. Exposure analysis (Cmax demonstrated that ~370-fold higher levels of EMS than that ingested by patients, are needed to saturate known, highly conserved, error-free, mammalian DNA repair mechanisms for alkylation. In summary, animal studies suggested that patients who took nelfinavir mesylate with elevated levels of EMS are at no increased risk for carcinogenicity or teratogenicity over their background risk, since mutations are prerequisites for such downstream events. These findings are potentially relevant to >40 marketed drugs that are mesylate salts.

  10. An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA (United States)

    Santos, Ana Paula de Torres; Levi, José Eduardo; Lemos, Marcilio Figueiredo; Calux, Samira Julien; Oba, Isabel Takano; Moreira, Regina Célia


    This study aimed to standardise an in-house real-time polymerase chain reaction (rtPCR) to allow quantification of hepatitis B virus (HBV) DNA in serum or plasma samples, and to compare this method with two commercial assays, the Cobas Amplicor HBV monitor and the Cobas AmpliPrep/Cobas TaqMan HBV test. Samples from 397 patients from the state of São Paulo were analysed by all three methods. Fifty-two samples were from patients who were human immunodeficiency virus and hepatitis C virus positive, but HBV negative. Genotypes were characterised, and the viral load was measure in each sample. The in-house rtPCR showed an excellent success rate compared with commercial tests; inter-assay and intra-assay coefficients correlated with commercial tests (r = 0.96 and r = 0.913, p < 0.001) and the in-house test showed no genotype-dependent differences in detection and quantification rates. The in-house assay tested in this study could be used for screening and quantifying HBV DNA in order to monitor patients during therapy. PMID:26872342

  11. High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform

    DEFF Research Database (Denmark)

    Fordyce, Sarah L; Avila-Arcos, Maria C; Rockenbauer, Eszter;


    The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that are used to estimate the number of STR...... repeat units. These methods do not allow for the full resolution of STR base composition that sequencing approaches could provide. Here we present an STR profiling method based on the use of the Roche Genome Sequencer (GS) FLX to simultaneously sequence multiple core STR loci. Using this method...

  12. Effect of specimen type on free immunoglobulin light chains analysis on the Roche Diagnostics cobas 8000 analyzer. (United States)

    Nelson, Louis S; Steussy, Bryan; Morris, Cory S; Krasowski, Matthew D


    The measurement of free immunoglobulin light chains is typically performed on serum; however, the use of alternative specimen types has potential benefits. Using the Freelite™ kappa and lambda free light chains assay on a Roche Diagnostics cobas 8000 c502 analyzer, we compared three specimen types (serum, EDTA-plasma and lithium heparin plasma separator gel-plasma) on 100 patients. Using Deming regression and eliminating outliers (limiting data to light chain concentrations below 400 mg/L), the three specimen types showed comparable results for kappa light chain concentration, lambda light chain concentration, and kappa/lambda ratio with slopes close to 1.0 and y-intercepts close to zero. EDTA-plasma showed slightly more positive bias relative to serum than lithium heparin. Analysis using EDTA-plasma and lithium heparin plasma showed comparable linearity, precision, and temperature stability. A single sample showing hook effect (not in the comparison set) gave comparable results using either plasma specimen type. For the Freelite™ kappa and lambda free light chains assay, both EDTA-plasma or lithium heparin-plasma can serve as acceptable substitutes for serum, at least for the Roche cobas 8000 analyzer.

  13. CCD Photometry and Roche Modeling of the Eclipsing Overcontact Binary Star System TYC 01963-0488-1 (United States)

    Alton, K. B.


    TYC 01963-0488-1 (ASAS J094440+2632.1) is a W UMa binary system (P=0.427036 d) which has been largely overlooked since first being detected nearly 15 years ago by the ROTSE-I telescope. Other than the monochromatic ROTSE-I survey data, no multi-colored light curves (LC) have been published. Photometric data collected in three bandpasses (B, V and Ic) at UnderOak Observatory (UO) produced 5 new times-of-minimum for TYC 01963-0488-1 which were used to establish a linear ephemeris from the first Min I epoch (HJD0). No published radial velocity data are available for this system; however, since this W UMa binary undergoes very obvious total eclipses, Roche modeling yielded a well-constrained photometric value for q ( 0.25). There is a suggestion from the ROTSE-I data and new results herein that Max II is more variable than Max I. Therefore, Roche model fits for the TYC 01963-0488-1 LCs collected in 2015 were assessed with and without spots.

  14. El teorema de Riemann-Roch y el morfismo de Gysin en geometría aritmética


    Navarro Garmendia, Alberto


    Le th eor eme de Riemann-Roch originale a rme que pour tout morphisme propre f : Y ! X entre vari et es quasi-projectifs lisses sur un corps, et tout el ement a 2 K0(Y ) du groupe de Grothendieck des br es vectoriels on a ch(f!(a)) = f {u100000}Td(Tf ) ch(a) (cf. [BS58]). Ici ch est le caract ere de Chern, Td(Tf ) est la classe de Todd du br e tangent relative et f et f! sont les images directes de l'anneau de Chow et K0 respectivement. Apr es, Baum, Fulton et MacPherson ont d emontr e...

  15. Clinical performance of the new Roche COBAS (R) TaqMan HCV test and high pure system for extraction, detection and quantitation of HCV RNA in plasma and serum

    NARCIS (Netherlands)

    H.C. Gelderblom; S. Menting; M.G. Beld


    We evaluated the Roche COBAS (R) TaqMan HCV Test For Use With The High Pure System (TaqMan HPS; Roche Diagnostics), for the extraction, detection and quantitation of hepatitis C virus (HCV) RNA in serum or plasma of HCV-infected individuals. The TaqMan HPS is a real-time PCR assay with a reported li


    Energy Technology Data Exchange (ETDEWEB)

    Benvenuto, O. G.; De Vito, M. A. [Facultad de Ciencias Astronómicas y Geofísicas, Universidad Nacional de La Plata and Instituto de Astrofísica de La Plata (IALP), CCT-CONICET-UNLP. Paseo del Bosque S/N (B1900FWA), La Plata (Argentina); Horvath, J. E., E-mail:, E-mail: [Instituto de Astronomia, Geofísica e Ciências Atmosféricas, Universidade de São Paulo R. do Matão 1226 (05508-090), Cidade Universitária, São Paulo SP (Brazil)


    We study the evolution of close binary systems formed by a normal (solar composition), intermediate-mass-donor star together with a neutron star. We consider models including irradiation feedback and evaporation. These nonstandard ingredients deeply modify the mass-transfer stages of these binaries. While models that neglect irradiation feedback undergo continuous, long-standing mass-transfer episodes, models including these effects suffer a number of cycles of mass transfer and detachment. During mass transfer, the systems should reveal themselves as low-mass X-ray binaries (LMXBs), whereas when they are detached they behave as binary radio pulsars. We show that at these stages irradiated models are in a Roche lobe overflow (RLOF) state or in a quasi-RLOF state. Quasi-RLOF stars have radii slightly smaller than their Roche lobes. Remarkably, these conditions are attained for an orbital period as well as donor mass values in the range corresponding to a family of binary radio pulsars known as ''redbacks''. Thus, redback companions should be quasi-RLOF stars. We show that the characteristics of the redback system PSR J1723-2837 are accounted for by these models. In each mass-transfer cycle these systems should switch from LMXB to binary radio pulsar states with a timescale of approximately one million years. However, there is recent and fast growing evidence of systems switching on far shorter, human timescales. This should be related to instabilities in the accretion disk surrounding the neutron star and/or radio ejection, still to be included in the model having the quasi-RLOF state as a general condition.

  17. Paleotectonic Settings for Petroleum Source Rocks Position paléolithique des roches mères

    Directory of Open Access Journals (Sweden)

    Curtis D. M.


    Full Text Available Petroleum source rocks have been deposited in a variety of paleotectonic and paleogeographic settings. The paleotectonic setting for a given source rock is, in many cases, different from the present tectonic setting of the basin in which the derived hydrocarbon accumulations are found. The quantity and type of hydrocarbons generated from a given source rock depend upon the quantity (richness, quality (kerogen type, and maturity (ability to generate hydrocarbons of the organic carbon in it. These three factors are strongly influenced by the paleotectonic setting of the basin in which a given source rock was deposited, as well as by its subsequent burial history (time-temperature history. The evolution of traps, on the other hand, depends upon the tectonic history of the basin during migration and entropment, and is closely related to the present tectonic setting ot the basin. Using a basin classification based on that of A. W. Bally and modified by K. Arbenz, I have attempted to related the distribution of hydrocarbon reserves in some of the major well-explored basins of the world to the paleotectonic settings for known or postulated source rocks in those basins. The principal basin categories defined are: cratonic, passive margin-related, aulacogenic. A-subduction-related, B-subduction-related, and strike-slip, transform-related. Preliminary results suggest that some of the best source rocks were deposited in aulacogenic and strike-slip/transform-related settings; some of the poorest were deposited in cratonic settings. Les roches mères de pétrole se sont déposées dans des sites paléotectoniques et paléogéographiques variés. La position paléotectonique d'une roche mère est dans de nombreux cas différente de la situation tectonique actuelle du bassin où l'on trouve les hydrocarbures qui en proviennent. La quantité et le type des hydrocarbures produits par une roche mère donnée dépendent de l'abondance (richesse, de la qualit

  18. Testing single-grain quartz OSL methods using sediment samples with independent age control from the Bordes-Fitte rockshelter (Roches d'Abilly site, Central France)

    DEFF Research Database (Denmark)

    Thomsen, Kristina Jørkov; Murray, Andrew Sean; Buylaert, Jan-Pieter;


    We present quartz single-grain dose distributions for four well-bleached and unmixed sediment samples with independent age control (22–48 ka), from the archaeologically important Bordes-Fitte rockshelter at Roches d'Abilly, France. This site has previously been dated using 14C AMS dating and stan...

  19. Comparison of Roche MONITOR and Organon Teknika NucliSens assays to quantify human immunodeficiency virus type 1 RNA in cerebrospinal fluid. (United States)

    Spearman, P; Fiscus, S A; Smith, R M; Shepard, R; Johnson, B; Nicotera, J; Harris, V L; Clough, L A; McKinsey, J; Haas, D W


    We compared Roche MONITOR and Organon Teknika NucliSens assays for human immunodeficiency virus type 1 (HIV-1) RNA in cerebrospinal fluid (CSF). Results of 282 assays were highly correlated (r = 0.826), with MONITOR values being 0.29 +/- 0.4 log(10) copies/ml (mean +/- standard deviation) values. Both assays can reliably quantify HIV-1 RNA in CSF.

  20. Performance of the Roche second generation hemoglobin A1c immunoassay in the presence of HB-S or HB-C traits. (United States)

    Abadie, Jude M; Koelsch, Angela A


    Blood HbA1c determination is a powerful tool for the evaluation and management of patients with diabetes mellitus. Many HbA1c analytical methods demonstrate bias in samples from patients with hemoglobinopathies. This study evaluated the analytical performance of Roche Diagnostics' 1st and 2nd generation HbA1c assays in patients with or without hemoglobinopathies whose HbA1c levels were elevated or normal, respectively. Boronate-affinity high performance liquid chromatography (HPLC) served as the reference method. Whole blood samples were collected from 80 patients with HbS or HbC whose group mean HbA1c value was elevated and also from 80 patients without hemoglobinopathy whose HbA1c values were in the well-controlled range. Each sample was assayed for HbA1c by the Primus boronate-affinity HPLC technique and by Roche's 1st and 2nd generation immunoassays using a Cobas Integra 800 analytical system. Results by the HPLC technique were compared with the results of both Roche assays by linear regression and Bland-Altman analysis. The 1st and 2nd generation assays yielded regression lines and correlation values vs HPLC assay of y = 1.43x - 1.59; R(2) = 0.83, and y = 0.94x + 0.10; R(2) = 0.92, respectively, in the 80 patients with hemoglobinopathies. The mean difference and the +/-2SD range were greater in the 1st than in the 2nd generation assay (2.68, +/-2.07 vs -0.54, +/-0.86, respectively). The 2nd generation assay also showed better performance than the 1st generation assay in samples from the 80 patients without hemoglobinopathy. In conclusion, this study validates the accuracy of Roche's 2nd generation assay, which is substantially improved over Roche's 1st generation HbA1c assay.

  1. Verification of analytical measurement range of serum creatinine detected by Roche Cobas 501 Biochemistry Analyzer%Roche Cobas 501生化分析仪血清肌酐分析测量范围的验证

    Institute of Scientific and Technical Information of China (English)

    陈永传; 崔亚利; 李艳; 任飒爽


    目的:通过对血清肌酐分析测量范围(AMR)的验证,探讨临床实验室如何按照国际标准要求进行生化分析仪定量检测项目分析测量范围的验证,保证检验结果准确、可靠。方法采用酶法在Roche Cobas 501生化分析仪上检测7个浓度水平美国病理学家协会(CAP)线性范围能力测试样品,这7个样品靶值涵盖厂家说明书标示肌酐分析测量范围低、中、高值,每个样品检测两次取其均值,计算其与靶值的偏倚。另外参照美国临床和实验室标准协会(CLSI )指南文件 EP6‐P的要求,收集含高值肌酐的新鲜患者血清,按一定比例混合、离心,计算混合物的浓度并将之作为高值样品(H ),与经同样处理获得的低值样品(L )分别按5L、4L+1H、3L+2H、2L+3H、1L+4H、5H的关系配制,形成系列样品,在Roche Cobas 501生化分析仪上对各样品的肌酐进行检测,每个样品检测4次,数据进行回归分析。结果7个水平的CAP样品与靶值的偏倚均小于北京善方医院检验科设定的允许误差±7.5%[(1/2×TE)%]。新鲜患者混合血清样品回归方程为Y=0.9886X+16.614,b=0.9886,介于0.97~1.03,截距a与0经 t检验,ta< t0.05,P>0.05,说明截距与0无明显差异,回归直线事实上通过0点。结论厂家说明书标示的血清肌酐分析测量范围验证通过,实验室可以采用。%Objective To investigate how the clinical laboratory conducting the verification of analytical measurement range (AM R) of quantitative items detected by the biochemical analyzer according to the requirements of the international standards by verifying the serum creatinine AMR for ensuring the accuracy and reliability of detection results .Methods The enzyme method was adopted to detect the 7‐concentration levels test specimens of CAP linear range proficiency test on the Roche Cobas 501 biochemical

  2. Neisseria gonorrhoeae False-Positive Result Obtained from a Pharyngeal Swab by Using the Roche cobas 4800 CT/NG Assay in New Zealand in 2012


    Upton, Arlo; Bromhead, Collette; Whiley, David M


    The Roche cobas 4800 CT/NG assay is a commonly used commercial system for screening for Neisseria gonorrhoeae infection, and previous studies have shown the method to be highly sensitive and specific for urogenital samples. We present the first confirmed clinical N. gonorrhoeae false-positive result using the cobas 4800 NG assay, obtained from testing a pharyngeal swab sample and caused by cross-reaction with a commensal Neisseria strain.

  3. From Roche Vitamins to DSM Nutritional Products%从罗氏维生素到帝斯曼营养产品

    Institute of Scientific and Technical Information of China (English)


    In October 2003, the Dutch group DSM has acquired the Vitamins and Fine Chemicals business from Roche to create DSM Nutritional Products-one of the world's leading suppliers of vitamins and carotenoids for Animal Nutrition industry, the Food and Pharmaceutical industries as well as the Cosmetics industry by offering a wide range of products to help improve nutrition and prevent disease. In China market, B vitamins are provided by DSM Nutritional Products for food fortification.

  4. Performance evaluation of the new Roche cobas AmpliPrep/cobas TaqMan HCV test, version 2.0, for detection and quantification of hepatitis C virus RNA

    NARCIS (Netherlands)

    S.D. Pas (Suzan); R. Molenkamp (Richard); J. Schinkel (Janke); S. Rebers; C. Copra (Cederick); S. Seven-Deniz; D. Thamke (Diana); R.J. de Knegt (Robert); B.L. Haagmans (Bart); M. Schutten (Martin)


    textabstractTo evaluate the analytical performance and explore the clinical applicability of the new Roche cobas AmpliPrep/cobas TaqMan HCV test, v2.0 (CAP/CTM v2.0), a platform comparison was performed on panels and diagnostic samples with the Roche cobas AmpliPrep/cobas TaqMan HCV test (CAP/CTM v1

  5. Analysis of 16S rRNA amplicon sequencing options on the Roche/454 next-generation titanium sequencing platform.

    Directory of Open Access Journals (Sweden)

    Hideyuki Tamaki

    Full Text Available BACKGROUND: 16S rRNA gene pyrosequencing approach has revolutionized studies in microbial ecology. While primer selection and short read length can affect the resulting microbial community profile, little is known about the influence of pyrosequencing methods on the sequencing throughput and the outcome of microbial community analyses. The aim of this study is to compare differences in output, ease, and cost among three different amplicon pyrosequencing methods for the Roche/454 Titanium platform METHODOLOGY/PRINCIPAL FINDINGS: The following three pyrosequencing methods for 16S rRNA genes were selected in this study: Method-1 (standard method is the recommended method for bi-directional sequencing using the LIB-A kit; Method-2 is a new option designed in this study for unidirectional sequencing with the LIB-A kit; and Method-3 uses the LIB-L kit for unidirectional sequencing. In our comparison among these three methods using 10 different environmental samples, Method-2 and Method-3 produced 1.5-1.6 times more useable reads than the standard method (Method-1, after quality-based trimming, and did not compromise the outcome of microbial community analyses. Specifically, Method-3 is the most cost-effective unidirectional amplicon sequencing method as it provided the most reads and required the least effort in consumables management. CONCLUSIONS: Our findings clearly demonstrated that alternative pyrosequencing methods for 16S rRNA genes could drastically affect sequencing output (e.g. number of reads before and after trimming but have little effect on the outcomes of microbial community analysis. This finding is important for both researchers and sequencing facilities utilizing 16S rRNA gene pyrosequencing for microbial ecological studies.

  6. Contribution à l'étude de la "taille" des roches Contribution to the Study of Rock Cutting

    Directory of Open Access Journals (Sweden)

    Nguyenminh D.


    Full Text Available Une étude expérimentale détaillée du processus local de destruction des roches par un outil de forage pétrolier a été faite par une méthode de similitude utilisant des matériaux équivalents tendres. On examine l'influence de la forme de l'outil, de la rugosité du contact et de la ductilité e globale » du matériau. Les résultats obtenus sont ensuite comparés à ceux de l'analyse plastique par la théorie des charges limites. Cette étude met en évidence l'existence de deux modes de rupture, l'action différente du frottement superficiel selon le sens de l'écoulement relatif du matériau en contact, le phénomène de la ductilité apparente qui dépend de la forme de l'outil ; elle précise les paramètres mécaniques importants du forage. A detailed experimental investigation of the local process of rock cutting by a drill bit was carried out on soft equivalent materials simulating rock under borehole conditions. Different factors were analyzed : the shape of the bit tooth, the roughness of ifs contact area, the average ductility of the materiat. The results obtained are then compared with those from a plastic analysis based on the peak lood theory. This investigation shows that there are two different modes of rock destruction, that the Coulomb friction has a different effect depending on the relative direction of flow of the materiat in contact with the bit, and that an apparent ductility of the materiat is linked to the shape of the bit teeth. Finally, the basic mechanical variables for drilling are described

  7. Multicenter comparison study of both analytical and clinical performance across 4 Roche HCV RNA assays utilizing different platforms. (United States)

    Vermehren, Johannes; Stelzl, Evelyn; Maasoumy, Benjamin; Michel-Treil, Veronique; Berkowski, Caterina; Marins, Ed G; Paxinos, Ellen E; Marino, Enrique; Wedemeyer, Heiner; Sarrazin, Christoph; Kessler, Harald H


    Antiviral treatment efficacy for chronic HCV infection is determined based on measurement of HCV RNA at specific time points throughout therapy by highly sensitive and accurate HCV RNA assays. This study evaluated the performance of two recently-developed real-time PCR HCV RNA assays, the cobas HCV for use on the cobas 6800/8800 systems (cobas 6800/8800 HCV) and the cobas HCV for use on the cobas 4800 system (cobas 4800 HCV) in comparison to two established assays, the COBAS AmpliPrep/COBAS TaqMan HCV Quantitative Test, version 2.0 (CAP/CTM v2) and the COBAS TaqMan HCV Test, version 2.0 for use with the High Pure System (HPS/CTM v2). Limit of detection (LOD) and linearity at lower concentrations (5-1000 IU/mL) were assessed for cobas 6800/8800 HCV and cobas 4800 HCV using WHO standard traceable panels representing HCV genotypes (GT) 1-4. Pairwise assay comparisons were also performed using 245 clinical samples representing HCV GT 1-4.cobas 6800/8800 HCV and cobas 4800 HCV were linear at low HCV RNA concentrations (<0.3 log10 IU/mL difference between expected and observed results) with LOD of 8.2 IU/mL and 11.7 IU/mL, respectively, for GT1. The new assays showed excellent agreement with CAP/CTM v2 and HPS/CTM v2 results in samples with quantifiable viral load. Concordance across the 6 million IU/mL cutoff was high among all four assays (90-94%). In conclusion, both cobas 6800/8800 HCV and cobas 4800 HCV tests are sensitive and linear, and correlate well with established Roche assays used in clinical practice.

  8. CCD Photometry, Roche Modeling and Evolutionary History of the WUMa-type Eclipsing Binary TYC01664-0110-1 (United States)

    Alton, K. B.; Stępień, K.


    TYC 01664-0110-1 (ASAS J212915+1604.9), a W UMa-type variable system (P=0.282962 d), was first detected over 17 years ago by the ROTSE-I telescope. Photometric data (B, V and Ic) collected at UnderOak Observatory (UO) resulted in five new times-of-minima for this variable star which were used to establish a revised linear ephemeris. No published radial velocity (RV) data are available for this system. However, since this W UMa binary undergoes a total eclipse, Roche modeling based on the Wilson-Devinney (W-D) code yielded a well-constrained photometric value for M2/M1 (q=0.356±0.001). There is a suggestion from ROTSE-I (1999) and ASAS survey data (2003, 2005, and 2008) that the secondary maximum is more variable than the primary one probably due to the so-called O'Connell effect. However, peak asymmetry in light curves (LC) from 2015 was barely evident during quadrature. Therefore, W-D model fits of these most recent data did not yield any substantive improvement with the addition of spot(s). Using the evolutionary model of cool close binaries we searched for a possible progenitor of TYC 01664-0110-1. The best fit is obtained if the initial binary has an orbital period between 3.3-3.8 d and component masses between 1.0-1.1 M⊙ and 0.30-0.35 M⊙. The model progenitor needs about 10 Gyr to attain the presently observed parameters of the variable. Its period slowly increases and the mass ratio decreases. According to the model predictions TYC 01664-0110-1 will go through the common envelope (CE) phase in the future, followed by merging of both components or formation of a double degenerate. Due to its apparent brightness (mV,max≍10.9 mag) and unique properties, the star is an excellent target for spectroscopic investigation of any possible deviations from a simple static model of a contact binary.

  9. Gyrotropy and Anisotropy of Rocks: Similarities and Differences Gyrotropie et anisotropie des roches : similitudes et différences

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    Chichinina T. I.


    Full Text Available The main features of wave propagation in gyrotropic media are compared with wave propagation in anisotropic media. The results of numerical modelling are presented for three typical seismic exploration models. The first two models are azimuthally anisotropic media (of orthorombic symmetry system, group 2m without and with gyration. The third model is a gyrotropic transversely isotropic medium with a vertical symmetry axis. The computations have been made for propagation of shear waves along the vertical symmetry axis. For sufficiently short wave paths (in our models less than 400 m the two-component (x, y seismograms are similar for all three models. For longer paths both signal shape and signal duration for the first and the third model differ noticeably. Some evidence (experimental data and a micromodel is given to show that the gyrotropy of rocks does exist or, at least, can exist. Les principales caractéristiques de la propagation des ondes dans les milieux gyrotropes sont comparées avec la propagation des ondes dans les milieux azimutalement anisotropes. Les résultats d'une modélisation numérique sont présentés pour trois modèles caractéristiques d'exploration sismique. Les deux premiers modèles sont des milieux anisotropes (de symétrie orthorhombique, groupe 2m avec et sans gyrotropie. Le troisième modèle est un milieu gyrotrope transverse isotrope avec un axe de symétrie vertical. Ces calculs ont été réalisés pour la propagation des ondes transversales le long de l'axe de symétrie vertical. Pour des trajets sismiques suffisamment courts (pour nos modèles, moins de 400 m, les sismogrammes à deux composantes (x, y sont similaires pour les trois modèles. Pour des trajets plus longs, la forme et la durée du signal diffèrent sensiblement pour les modèles 1 et 3. Ceci a pour but de montrer (à l'aide des données expérimentales et d'un micromodèle que la gyrotropie dans les roches existe, ou, tout au moins, peut

  10. Aprovechamiento del salto de Bourg-Lés-Valence. La Presa de la Roche de Glun-Drôme – Francia

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    Française d´Enterprise, Compagnie


    Full Text Available To take the fullest advantage of the hydroelectric and agricultural usefulness of the Rhône, the Compagnie Nationale du Rhône has divided the river into a number of sections, and these have been separately developed. The hydraulic gradient at La Roche de Glun, at the end of the central section, involves a hydraulic system consisting of a reservoir, a dam and dykes, a canal, a power station, an overflow canal and drainage works in the , surrounding zones. This project has been carried out by the Compagnie Française d'Enterprises.Para explotar al máximo las posibilidades hidroeléctricas y agrícolas del Ródano, la Compagnie Nationale du Rhône ha dividido dicho río en una serie de tramos para proceder a su distribución. El salto de La Roche de Glun, último del tramo central, forma un complejo hidráulico que consta de: un embalse cerrado por diques, una presa, un canal de alimentación, una central, una esclusa, un canal de descarga y unas obras anejas de drenaje para evitar inundaciones en las ciudades próximas. Las obras han sido realizadas por la Compagnie Française d'Entreprises.

  11. Method comparison of the Ortho Vitros Fusion 5,1 chemistry analyzer and the Roche COBAS Integra 400 for urine drug screen testing in the emergency department. (United States)

    Johnson-Davis, Kamisha L; Thompson, Catherine D; Clark, Chantry J; McMillin, Gwen A; Lehman, Christopher M


    Exposure to drugs and toxins is a major cause for the rising number of emergency department visits each year. Immunoassays are commonly used in the emergency department to provide rapid turnaround time for acute care. The purpose of this study was to compare two automated immunoassay chemistry analyzers to determine which platform produced the fewest number of false positive/negative results. Residual patient urine samples were were collected for each of the following drugs/drug classes: cocaine (n = 40), opiates (n = 45), and amphetamines (n = 54) and confirmed either positive or negative by mass spectrometry. Split sample analyses of these specimens were performed on both the Roche COBAS INTEGRA 400 plus and Ortho Vitros 5,1 FS instruments. The results from the two chemistry analyzers were compared to confirmed results. Both immunoassays were prone to false positive results for cocaine and false negative results for opiates and amphetamines. The Vitros Fusion analyzer generated fewer false positive and false negative results for opiate and amphetamine testing than the Roche Integra, but the platforms performed comparably for cocaine.

  12. Une nouvelle caractérisation des fondations rocheuses pour une meilleure approche de la résistance au cisaillement d'une interface béton-roche


    MOUZANNAR, Hussein; BOST, Marion; JOFFRIN, Patrick


    La construction d'un barrage-poids sur une fondation rocheuse nécessite une justification de la stabilité vis-à-vis du glissement à l'interface entre béton et roche. Or les nombreuses recherches sur le comportement au cisaillement d'une discontinuité rocheuse ont mis en évidence que le comportement dépend en partie de la morphologie de la surface rocheuse. L'étude du comportement au cisaillement d'une interface béton-roche doit donc comprendre la caractérisation de la rugosité de la surface r...

  13. Field evaluation of Abbott Real Time HIV-1 Qualitative test for early infant diagnosis using dried blood spots samples in comparison to Roche COBAS Ampliprep/COBAS TaqMan HIV-1 Qual test in Kenya. (United States)

    Chang, Joy; Omuomo, Kenneth; Anyango, Emily; Kingwara, Leonard; Basiye, Frank; Morwabe, Alex; Shanmugam, Vedapuri; Nguyen, Shon; Sabatier, Jennifer; Zeh, Clement; Ellenberger, Dennis


    Timely diagnosis and treatment of infants infected with HIV are critical for reducing infant mortality. High-throughput automated diagnostic tests like Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Qual Test (Roche CAPCTM Qual) and the Abbott Real Time HIV-1 Qualitative (Abbott Qualitative) can be used to rapidly expand early infant diagnosis testing services. In this study, the performance characteristics of the Abbott Qualitative were evaluated using two hundred dried blood spots (DBS) samples (100 HIV-1 positive and 100 HIV-1 negative) collected from infants attending the antenatal facilities in Kisumu, Kenya. The Abbott Qualitative results were compared to the diagnostic testing completed using the Roche CAPCTM Qual in Kenya. The sensitivity and specificity of the Abbott Qualitative were 99.0% (95% CI: 95.0-100.0) and 100.0% (95% CI: 96.0-100.0), respectively, and the overall reproducibility was 98.0% (95% CI: 86.0-100.0). The limits of detection for the Abbott Qualitative and Roche CAPCTM Qual were 56.5 and 6.9copies/mL at 95% CIs (p=0.005), respectively. The study findings demonstrate that the Abbott Qualitative test is a practical option for timely diagnosis of HIV in infants.

  14. Performance evaluation of Roche LightCycler Nano fluorogenic quantitative PCR instrument%罗氏LightCyclerNano荧光定量PCR仪性能评价

    Institute of Scientific and Technical Information of China (English)

    张宇; 李冬; 戴燕; 郑为超; 万海英


    目的:对美国罗氏公司LightCycler Nano 32孔荧光定量聚合酶链反应(PCR)仪的主要性能指标进行评价。方法按照美国临床实验室标准化协会(CLSI)制定的评价标准,评价仪器精密度、准确度、灵敏度、可报告范围、仪器间比对等指标。结果 LightCycler Nano 精密度批内变异系数(CV )为2.63%、1.51%,批间 CV 为6.2%、4.15%;准确度与室间质控物比较,5个标本均在靶值区间内;灵敏度检测 CV≤10%;可报告范围为最大稀释比例1∶100;与比对仪器的比对结果偏差结果均小于15%。结论罗氏LightCycler Nano荧光定量PCR仪5项性能经验证后与厂家提供的性能参数相符,可以用于临床检测。%Objective To evaluate the major performance indexes of American Roche LightCycler Nano 32 holes fluorogenic quantitative PCR instrument .Methods According to the evaluation standard formulated by the Clinical and Laboratory Standars Institute(CLSI) ,the precision ,accuracy ,sensitivity ,reportable range and compari‐son between the instruments were evaluated .Results The coefficients of variation(CV) for within‐run assays were 2 .63% and 1 .51% respectively ;CV for between‐run assays were 6 .2% and 4 .15% respectively ;comparing the accu‐racy with the between‐run quality control material ,the five samples were within the target value range;CV for sensi‐tivity was ≤ 10% ;the reportable range of the maximum dilution was 1∶100;the bias of the comparison results with the comparative instrument was less than 15% .Conclusion The verified 5 items of performance in the Roche Light‐Cycler Nano fluorogenic quantitative PCR instrument conform to the performance parameters provided by the manu‐facturer ,indicating that this instrument can be applied in clinical testing in our laboratory .

  15. Diffusion Properties of Carbonated Caprocks from the Paris Basin Propriétés diffusives de roches de couverture carbonatées du Bassin de Paris

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    Berne P.


    carbonated caprocks (regardless of the problem of alteration by acidified water, not addressed here. Lastly, a few simple rules for extending the results presented here to other experimental conditions are proposed. L’efficacite d’un stockage geologique de CO2 repose sur les proprietes de confinement des roches de couverture. Parmi ces proprietes, les caracteristiques diffusives jouent un role important qui est parfois sous-estime. En effet, la diffusion est un processus lent mais qui peut s’averer non negligeable sur le long terme. De plus, les caracteristiques recherchees pour une roche de couverture, principalement une forte pression d’entree et une faible permeabilite, ne sont pas incompatibles avec des coefficients de diffusion relativement eleves, puisque ces grandeurs sont liees a des caracteristiques geometriques differentes (schematiquement, les tailles de pore pour les unes et la tortuosite pour les autres. Dans le cadre du projet Geocarbone–INTEGRITE, finance par l’ANR de 2006 a 2008, on a donc mesure les coefficients de diffusion de l’eau et de l’ion bicarbonate dans des echantillons de roche de couvertures carbonatees provenant de trois niveaux (formations des Calcaires du Comblanchien et de la Dalle Nacree. Ces deux especes permettent en effet de couvrir, en premiere approximation, les situations rencontrees en conditions de stockage (espece neutre/espece chargee, interagissant ou non avec la matrice solide. Dans un premier temps, on presente le modele simple de diffusion utilise pour le depouillement des experiences, ce qui permet de passer en revue les differentes grandeurs utiles : coefficient de diffusion effectif, coefficient de diffusion de pore, coefficient de diffusion apparent, porosite accessible, facteur de retard. Le coefficient d’autodiffusion de l’eau est mesure par deux techniques differentes (Resonance Magnetique Nucleaire et tracage radioactif a l’eau tritiee 1H3HO dans deux echantillons. Les resultats obtenus sont

  16. The Bolivian Source Rocks. Sub Andean Zone. Madre de Dios. Chaco Les roches mères de Bolivie. Subandin. Madre de Dios. Chaco

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    Diaz Martinez E.


    connaissances sur les roches mères de Bolivie. Ce travail a été fait dans le cadre de la convention de coopération qui lie depuis 1987 YPFB et l'ORSTOM - Institut Français de Recherche Scientifique pour le Développement en Coopération. L'établissement de cette base de données a permis une synthèse du potentiel pétrolier du pays, la partie concernant le bassin de Madre de Dios, la zone subandine et le Chaco, c'est-à-dire toute la zone potentiellement pétrolière du pays, hors Altiplano, est ici présentée. La base contient actuellement les analyses d'un peu plus de 3000 échantillons. Les roches mères sont Paléozoïques et se sont déposées dans un contexte de bassin d'avant-pays; celui ci s'étendait sur la cordillère ainsi que sur l'avant-pays actuel du Silurien au Permien. Le bassin, restreint initialement à la zone sud-ouest, s'est ensuite étendu vers le nord. La bordure ouest n'est pas connue, elle devait se situer au niveau de l'Altiplano actuel. La bordure est correspond au bouclier Brésilien affleurant actuellement à l'est du pays et sur laquelle se biseaute le Paléozoique. La sédimentation est marine, il s'agit d'une plate-forme carbonatée s'approfondissant au Carbonifère. De nombreuses évidences de diamectites et de figures de resédimentation ont été notées à partir de la fin du Dévonien. Le Trias marque la fin de ces dépôts et se caractérise par une érosion, sans doute due à une phase de rifting avortée. La sédimentation Jurassique et Crétacée est presque toujours continentale et souvent éolienne, il n'y a pas de structuration marquée. Au nord, dans la zone de Madidi, les dépôts du Crétacé terminal (Maestrichian montrent des intercalations marines riches en matière organique (Fm Flora, alors que son équivalent au centre et au Sud (Fm Cajones est purement gréseux. Le soulèvement de la cordillère orientale démarre à la fin de l'Oligocène (27 Ma, Sempere et al. , 1990 et le front de compression se déplace ensuite

  17. Un habitat orné en abri sous-roche au Magdalénien Moyen, Angles-sur-l'Anglin (Vienne, France

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    Iakovleva, Ludmila


    Full Text Available Not Available.

    [fr] L'originalité du site du Roc-aux-Sorciers à Angles-sur l´Anglin dans la Vienne (France se traduit par la présence d'un art pariétal magdalénien sculpté dont une partie, disposée sur la paroi du fond de l'abri sous roche, forme une frise sur près de 18 mètres de long. L´ étude, tant thématique que technique ou stylistique, de cet art pariétal in situ démontre une organisation et une construction dans ce complexe figuratif sculpté. Les sujets, aussi bien dans leur traitement que dans leur rendu, répondent à des règles précises propres au site d'Angles. Ils se regroupent par espèce en s'associant à d'autres sujets animaliers ou humains dans des espaces nettement délimités formant ainsi une composition sculptée. Des zones figuratives rassemblant plusieurs de ces compositions sculptées assimilées à des "panneaux", sont mises en relation avec le positionnement de grands foyers installés sur la roche-mère. Cette analyse nous amène à mettre en valeur une structure complexe d'habitat en liaison avec cette frise pariétale. [es] Un hábitat decorado del Magdaleniense Medio en el abrigo de Angles-sur-l'Anglin (Vienne, France. La originalidad del yacimiento de Roc-aux-Sorciers en Angles-sur-l 'Anglin, en Vienne (Francia, se debe a la existencia de un arte parietal magdaleniense esculpido del que una parte, situada en la pared del fondo del abrigo, forma un friso de 18 m. de largo, aproximadamente. El estudio, tanto temático como técnico o estilístico, de este arte parietal in situ demuestra una organización en este complejo figurativo esculpido. Las figuras, tanto en su tratamiento como en su expresión, responden a reglas precisas, propias del yacimiento de Angles. Se agrupan por especies, asociándose a otras figuras animales o humanas, en unos espacios estrictamente limitados, formando así una composición esculpida. Las zonas figurativas que integran muchas de estas

  18. Comparison of GMT presto assay and Roche cobas® 4800 CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in dry swabs. (United States)

    de Waaij, Dewi J; Dubbink, Jan Henk; Peters, Remco P H; Ouburg, Sander; Morré, Servaas A


    Urogenital Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are the most prevalent bacterial STIs worldwide. Molecular tests are the standard for the detection of CT and NG, as these are difficult to culture. The recently introduced CE-IVD marked GMT Presto assay promises to be a valuable addition in CT and NG diagnostics. The advantage of the Presto assay is that it works on many PCR systems and the DNA can be isolated by any system.We compared the Presto assay to the widely used Roche cobas® 4800 CT/NG test for the detection of CT and NG in 612 vaginal and rectal dry collected swabs. Discrepant samples were tested by the TIB MOLBIOL Lightmix Kit 480 HT CT/NG assay. The alloyed gold standard was defined as two concurring Presto and cobas® 4800 results, or, with discrepant Presto and cobas® results, two concurring results of either test together with the Lightmix Kit 480 HT CT/NG assay. For the Presto assay,we observed 77 CT positive (13%) and 22 NG positive (3,6%) vaginal samples, and 41 CT positive (6,7%) and 11 NG positive (1,8%) rectal samples. For the cobas® 4800 assay,we observed 77 CT positive (13%) and 21NG positive (3,4%) vaginal samples, and 39 CT positive (6,4%) and 11 NG positive (1,8%) rectal samples. Ten CT samples were discrepant between Presto and cobas® 4800 CT/NG assays, while two NG samples were discrepant. CT sensitivity in both assays was 100% compared to the alloyed gold standard. The sensitivity was 100% for both vaginal and rectal dry swabs, underlining the suitability of these sample types for detection of CT and NG. The Presto assay is therefore valuable for molecular detection of CT and NG in dry vaginal and rectal swabs.

  19. 罗氏尿微量清蛋白检测试剂盒性能评价%Performance evaluation on Roche urine trace albumin detection reagent kit

    Institute of Scientific and Technical Information of China (English)

    黄勤烽; 黄少铃; 王美珠; 陈敏


    Objective To evaluate the detection performance of the cobas8000 c702 fully automatic biochemical analyzer for de‐tecting the second generation Roche urine trace albumin (ALBU2) .Methods (1) The precise evaluation :with the allowable error stipulated by CLIA 88 as the basis ,the requirements were the repeat precision <1/4TEa ,and intermediate precision <1/3TEa;(2) the linear range and the evaluation of the reportable range :the EP6‐A scheme was adopted ,and extend to calculate the average re‐covery rate of dilution ,the clinical reportable range was evaluated by the average dilution recovery of 90% -110% ;(3) the carry o‐ver pollution assessment :the carry over pollution of serum albumin on urine trace albumin detection was evaluated by the judgment standard of carry over pollution rate of 0 .5% ;(4)the methodological comparative analysis :with SIEMENS BN Ⅱas the reference system ,the Roche Cobas 8000 C702 and the BN2 results were performed the correlation contrastive analysis .Results The repeat precision :low concentration CV=1 .98% .high concentration CV=1 .64% ;intermediate precision :low concentration CV=4 .35% , high concentration CV=1 .20% ;the linear range verification :the measurement range 5 .6-413 .55 mg/L ;clinical reportable range :in the maximum diluted multiples of 30 times ,the clinical reportable range was 5 .6-12 406 .5 mg/L ;the carry over pollution rate :serum albumin (42 .6 g/L) on urine trace albumin(6 .9 mg/L) ,the carry over pollution rate was 0 .28% ;the indoor comparison :in the concentration within 200 mg/L ,the regression line was Y=0 .896 X+5 .049 ,the correlation coefficient r2 =0 .994 4 ,the system shift was passed at the medical decision level .When the specimen concentration within 201-413 .55 mg/L ,the regression line was Y=0 .848X-10 .44 ,the correlation coefficient r2 =0 .917 ,the system shift was not passed at the medical decision level .Conclusion The detection of the Roche ALBU2 in the Cobas 8000 C702 platform can

  20. Roche/BIOTECON Diagnostics LightCycler foodproof L. monocytogenes detection kit in combination with ShortPrep foodproof II Kit. Performance-Tested Method 070401. (United States)

    Junge, Benjamin; Berghof-Jäger, Kornelia


    A method was developed for the detection of L. monocytogenes in food based on real-time polymerase chain reaction (PCR). This advanced PCR method was designed to reduce the time needed to achieve results from PCR reactions and to enable the user to monitor the amplification of the PCR product simultaneously, in real-time. After DNA isolation using the Roche/BIOTECON Diagnostics ShortPrep foodproof II Kit (formerly called Listeria ShortPrep Kit) designed for the rapid preparation of L. monocytogenes DNA for direct use in PCR, the real-time detection of L. monocytogenes DNA is performed by using the Roche/BIOTECON Diagnostics LightCycler foodproof L. monocytogenes Detection Kit. This kit provides primers and hybridization probes for sequence-specific detection, convenient premixed reagents, and different controls for reliable interpretation of results. For repeatability studies, 20 different foods, covering the 15 food groups recommended from the AOAC Research Institute (AOAC RI) for L. monocytogenes detection were analyzed: raw meats, fresh produce/vegetables, processed meats, seafood, egg and egg products, dairy (cultured/noncultured), spices, dry foods, fruit/juices, uncooked pasta, nuts, confectionery, pet food, food dyes and colorings, and miscellaneous. From each food 20, samples were inoculated with a low level (1-10 colony-forming units (CFU)/25 g) and 20 samples with a high level (10-50 CFU/25 g) of L. monocytogenes. Additionally, 5 uninoculated samples were prepared from each food. The food samples were examined with the test kits and in correlation with the cultural methods according to U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) or U.S. Department of Agriculture (USDA)/Food Safety and Inspection Service (FSIS) Microbiology Laboratory Guidebook. After 48 h of incubation, the PCR method in all cases showed equal or better results than the reference cultural FDA/BAM or USDA/FSIS methods. Fifteen out of 20 tested food types

  1. Diagnosis of genital Chlamydia trachomatis infections in asymptomatic males by testing urine by PCR.


    Domeika, M; Bassiri, M; Mårdh, P A


    An enzyme-linked immunosorbent assay (EIA) (MikroTrak; Syva) was compared with PCR (Amplicor; Roche) for detection of Chlamydia trachomatis in first-void urine (FVU) from 184 men attending a skin and venereal disease clinic. The prevalence of C. trachomatis in the population studied was 18.5%. Discrepant results between Syva EIA and Roche PCR were retested by using major outer membrane protein primer-based PCR. After retesting, the sensitivity, the specificity, and the positive and negative p...

  2. Les roches basiques des boutonnieres d'Agadir melloul et d'Iguerda - taifast : tkmoins de l’histoire preorogenique de la chaine panafricaine de l’Anti-Atlas (Maroc

    Directory of Open Access Journals (Sweden)

    El Aouli, E. H.


    Full Text Available Dans les boutonnières précambriennes d'Agadir Melloul et d'Iguerda-Taïfast (Anti-Atlas central, les roches basiques du Néoprotérozoïque (PII sont représentées par des dykes et des corps basiques intrusifs dans le socle paléoprotérozoïque (PI et les quartzites du PII. L’ensemble PI, PII et roches basiques est affecté par la schistosité régionale panafricaine. Les éléments en traces, réputés peu mobiles au cours des processus d'altération et de métamorphisme (Nb, Y, Zr, Ti, V et les terres rares ont permis de subdiviser ces roches basiques en deux groupes: un groupe tholeiitique et un groupe transitionnel. Le cadre géodynamique de mise en place de ces roches pourrait être lié à un contexte distensif marquant le rifting pré-panafricain en relation avec l’ouverture océanique reconnue dans la boutonnière de Bou Azzer-El Grara et dans le massif de Siroua.En los complejos precámbricos de Agadir Melloul y de Iguarda-Taifast (Anti-Atlas centro, las rocas básicas del Neoproterozoico (PII están representadas por diques y cuerpos básicos intmsivos en el zócalo Paleoproterozoico (PI y las cuarcitas del PII. El conjunto PI, PII y las rocas básicas está afectado por la esquistosidad regional panafricana. Los elementos traza incompatibles, poco móviles en el transcurso de procesos de alteración y de metamorfismo (Nb, Y, Zr, Ti, V, así como las tierras raras, permiten subdividir estas rocas básicas en dos grupos: un grupo toleítico y otro transicional. El marco geodinámico de la intrusion de estas rocas podria estar ligado a un contexto extensional asociado al rifting pre-panafricano en relación con la apertura oceánica reconocida en el complejo de Bou Azzer-El Graara y en el macizo de Siroua.

  3. Application de la chromatographie sur couche mince à l'étude quantitative et qualitative des extraits de roches et des huiles Application of Thin-Layer Chromatography to the Quantitative and Qualitative Analysis of Rock and Oil Extracts

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    Huc A. Y.


    Full Text Available La technique développée ici répond à un besoin de miniaturisation des analyses des huiles et des extraits de roche. La chromatographie sur couche mince permet en effet l'analyse qualitative et quantitative de faibles quantités d'échantillons. On peut grace à cette méthode étudier les extraits obtenus à partir des cuttings (5 à 10 g de roche. Nous nous sommes attachés à faire une étude critique de l'information obtenue et de comparer cette dernière aux résultats fournis par les autres méthodes analytiques (chromatographie liquide. The technique described here meets the need to miniaturize analyses of ails and rock extracts. Thin-layer chromatography tan be used for the qualitative and quantitative analysis of small amounts of samples. This method is capable of onalyzing extracts from cuttings (5 ta 10 g of rock. This article attempts to make a critical study of the information obtained and to compare il with results using other analytical methods (liquid chromatography.

  4. Total vitamin D assay comparison of the Roche Diagnostics "Vitamin D total" electrochemiluminescence protein binding assay with the Chromsystems HPLC method in a population with both D2 and D3 forms of vitamin D. (United States)

    Abdel-Wareth, Laila; Haq, Afrozul; Turner, Andrew; Khan, Shoukat; Salem, Arwa; Mustafa, Faten; Hussein, Nafiz; Pallinalakam, Fasila; Grundy, Louisa; Patras, Gemma; Rajah, Jaishen


    This study compared two methods of assaying the 25-hydroxylated metabolites of cholecalciferol (vitamin D3) and ergocalciferol (vitamin D2). A fully automated electrochemiluminescence assay from Roche Diagnostics and an HPLC based method from Chromsystems were used to measure vitamin D levels in surplus sera from 96 individuals, where the majority has the D2 form of the vitamin. Deming regression, concordance rate, correlation and Altman Bland agreement were performed. Seventy two subjects (75%) had a D2 concentration >10 nmol/L while the remaining twenty four subjects had vitamin D2 concentration of less than 10 nmol/L by HPLC. Overall, the Roche Diagnostics method showed a negative bias of -2.59 ± 4.11 nmol/L on the e602 as compared to the HPLC with a concordance rate of 84%. The concordance rate was 91% in samples with D2 of less than 10 nmol/L and 82% in those with D2 concentration >10 nmol/L. The overall correlation had an r value of 0.77. The r value was higher in samples with D2 levels of less than 10 nmol/L, r = 0.96, as compared to those with D2 values of greater than 10 nmol/L, r = 0.74. The observed bias had little impact on clinical decision and therefore is clinically acceptable.

  5. Total Vitamin D Assay Comparison of the Roche Diagnostics “Vitamin D Total” Electrochemiluminescence Protein Binding Assay with the Chromsystems HPLC Method in a Population with both D2 and D3 forms of Vitamin D

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    Gemma Patras


    Full Text Available This study compared two methods of assaying the 25-hydroxylated metabolites of cholecalciferol (vitamin D3 and ergocalciferol (vitamin D2. A fully automated electrochemiluminescence assay from Roche Diagnostics and an HPLC based method from Chromsystems were used to measure vitamin D levels in surplus sera from 96 individuals, where the majority has the D2 form of the vitamin. Deming regression, concordance rate, correlation and Altman Bland agreement were performed. Seventy two subjects (75% had a D2 concentration >10 nmol/L while the remaining twenty four subjects had vitamin D2 concentration of less than 10 nmol/L by HPLC. Overall, the Roche Diagnostics method showed a negative bias of −2.59 ± 4.11 nmol/L on the e602 as compared to the HPLC with a concordance rate of 84%. The concordance rate was 91% in samples with D2 of less than 10 nmol/L and 82% in those with D2 concentration >10 nmol/L. The overall correlation had an r value of 0.77. The r value was higher in samples with D2 levels of less than 10 nmol/L, r = 0.96, as compared to those with D2 values of greater than 10 nmol/L, r = 0.74. The observed bias had little impact on clinical decision and therefore is clinically acceptable.

  6. Analyse géochimique de la matiére organique extraite des roches sédimentaires. IV. Extraction des roches en faible quantités Geochemical Analysis of Organic Matter Extracted from Sedimentary Rocks Iv. Exraction from Small Amounts of Rock

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    Monin J. C.


    Full Text Available L'extraction en Soxhlet est inappllcable lorsque les échantillons de roche sont de trop petite taille. A l'occasion de la mise au point du protocole d'extraction correspondant, on examine l'influence d'un certain nombre de conditions opératoires sur le rendement d'extraction : température, durée nature et quantité du solvant, présence de lumière, présence d'air, procédé d'extraction. Pour les hydrocarbures, tant saturés qu'aromatiques, le facteur essentiel est l'agitation du milieu d'extraction ; la nature du solvant n'est pas critique, à condition de ne pas choisir un très mauvais solvant des hydrocarbures : l'extractibilité est en effet plus fonction du pouvoir désorbant vis-à-vis de la roche que du pouvoir solvant proprement dit. Pour les résines et asphalténes, l'interprétation des résultats est délicate, car la frontière n'est pas nette entre produits simplement dissous, produits de solvolyse et, produits de néoformation par interaction solvant-matière organique-matière minérale. II n'existe donc pas de protocole d'extraction recommandable dans l'absolu. Tout dépend des exigences analytiques et aussi pratiques du laboratoire; à l'Institut Français du Pétrole (IFP le protocole retenu est l'extraction en bécher avec agitation magnétique pendant 20 min dans le chroroforme à 50 °C (approximativement; on donne aussi le protocole d'évaporation du solvant et de récupération de l'extrait, qui doit être étudié soigneusement étant donné les faibles quantités mises en jeu. A Soxhlet extractor cannot be used with rock samples that are too small in size. With the development of on extraction procédure for such cases, this article examines the influence of various operating conditions on extraction yield, i. e. temperature, duration, nature and amount of solvent, presence of light, présence of air and extraction process. For both saturated and aromatic hydrocarbons, the essential factor is the stirring of the

  7. ROCHE URYSIS-2400和AIKELAI AX-4030尿液干化学分析仪检测一致性的比较%Comparison on the consistency between ROCHE URYSIS-2400 and AIKELAI AX-4030 urine dry-chemistry analyzers

    Institute of Scientific and Technical Information of China (English)

    吴蕙; 王蓓丽; 郭玮; 潘柏申


    目的:比较ROCHE URYSIS-2400尿液干化学分析仪(简称URYSIS-2400)和AIKELAI AX-4030尿液干化学分析仪(简称AX-4030)的检测结果的一致性。方法分别用URYSIS-2400和AX-4030平行测定500例尿液常规标本,比较检测结果的阳性检出率和符合率。随机选取250例使用尿沉渣显微镜复核尿红细胞(ERY)和白细胞(LEU)。通过添加实验评估 URYSIS-2400、AX-4030抗维生素 C 干扰的能力。结果2台仪器比重(SG)差异百分率均值<1%,其余9项干化学检测项目一般符合率都>90%。URYSIS-2400和AX-4030 ERY项目与尿沉渣显微镜计数的符合率分别为82.4%、84.4%,LEU分别为76.0%和83.2%。URYSIS-2400、AX-4030葡萄糖(GLU)和ERY项目添加维生素C浓度达50 mg/dL时不受干扰。结论 URYSIS-2400和AX-4030检测结果一致性良好,但尿液干化学分析仪只是筛检仪器,还应结合显微镜尿沉渣计数复核的结果以满足临床需要。%Objective To compare the consistency of ROCHE URYSIS-2400 and AIKELAI AX-4030 urine dry-chemistry analyzers.Methods A total of 500 urine specimens were detected by the 2 kinds of urine chemistry analyzers in parallel,and the results were analyzed statistically by positive detection rates and accordance rates.A total of 250 cases were randomly selected for reviewing erythrocytes (ERY)and leulcocytes (LEU)by urine sediment microscopy. Experiments by adding vitamin C were used to evaluate URYSIS-2400 and AX-4030 anti-interference abilities against vitamin C.Results The mean difference percentage of specific gravity (SG)between the 2 analyzers was 90%.The consistency rates of URYSIS-2400 and AX-4030 were 82.4% and 84.4% with urine sediment microscopy on ERY,and 76.0% and 83.2% on LEU. URYSIS-2400 and AX-4030 anti-interference abilities against vitamin C were up to 50mg/dL on glucose (GLU)and ERY.Conclusions There is a good consistency between the results of URYSIS-2400 and AX

  8. Etude de l’activité pouzzolanique d’une roche andésitique en Algérie Study of the pozzolanic activity of an andesitic rock in Algeria

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    Clastres P.


    Full Text Available Ce travail s’inscrit dans le cadre de l’accord programme Tassili N° 09 MDU 773 entre le Laboratoire de Physicochimie des Matériaux, USTO-Oran et le Laboratoire des Matériaux et Durabilité des Constructions (LMDC, INSA-UPS-Toulouse, France. La présente étude est une contribution aux travaux de la recherche de nouvelles sources d’approvisionnement en ajouts et en matières premières et la valorisation des matériaux naturels locaux utilisés dans la fabrication du ciment. Cette étude porte sur l’activité pouzzolanique des roches volcaniques, elle a pour but de comparer les caractéristiques physiques et mécaniques d’un mortier témoin à ciment de type CEMI 52,5R fourni par la firme Lafarge et d’un mortier à ciment du même type avec ajout dont les taux de substitution de la roche Andésitique est de 0- 40%. Dans notre travail, nous avons utilisé des roches volcaniques appartenant à la famille des Andésites, provenant de la région de Tipaza, Nord Ouest Algérois. L’étude des caractéristiques mécaniques et de l’indice de pouzzolanicité ont montré que les ciments fabriqués sont capables de remplacer certains ciments Portland pour une éventuelle utilisation dans le domaine de construction. This work is a part of the agreement programs Tassili N° 09 MDU 773 between Laboratory of Physic-chemistry Materials, USTO-Oran and Laboratory of Materials and Durability of Constructions (LMDC, INSA-UPS-Toulouse, France. The present study is an attempt to the research of new supply sources in admixtures and raw materials and the evaluation of local natural materials used in the manufacture of cement. It deals with the pozzolanic activity of the volcanic rocks and aims to compare the physical and mechanical characteristics of a mortar control made with cement CEMI 52,5R provided by Lafarge firm and of a mortar made with the same cement incorporated by different replacement rate of andesitic rock in a range of 0 – 40

  9. Indices chimiques pour l'évaluation de l'état diagénétique des huiles et des roches sapropéliques Chemical Evidence for Evaluating the Diagenetic State of Sapropelic Oils and Rocks

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    Du Rouchet J.


    Full Text Available Les analyses chromatographiques des vapeurs produites par le chauffage à 220 °C d'huiles brutes et de roches mettent en évidence une augmentation avec la diagenèse des teneurs en n-alcanes relativement aux teneurs en cyclones et en iso-alcanes. Une étude statistique permet de préciser la regularite de l'evolution des rapports des teneurs en n-C 6 et en méthylcyclopentane etdes teneurs en n-C 7 et en I-trans-2-diméthylcyclopentane et met en évidence pour les roches une corrélation significative entre ces rapports et les réflectances de la vitrinite. Ces rapports peuvent donc être considérés comme des indicateurs diagénétiques; ils paraissent être moins dépendants des caractéristiques particulières de la matière organique originelle que les couples pristane-n-C 17 et phytane-n-C 18 habituellement utilisés pour repérer l'état d'évolution des huiles et des extraits de roche. Une calibration en termes de réflectance de la vitrinite est proposée. Chromatographic analyses of the vapors produced by crude cils and rocks heated to 220°C reveal, with diagenesis, an increase in the n-alkane contents with respect to the cyclone and isoalkane contents. A statistical survey shows the regularity of the variation in the ratios of the n-C 6 and methylcyclopentane contents and the n-C7 and I-trans-2-dimethylcyclopentane contents and reveals a meaningful corrélation for rocks between these ratios and the vitrinite reflectances. Therefore, these ratios may be considered as diagenetic indicators. They appear to depend less on the particular properties of the original organic motter thon on the pristane/n-C 17 and phytane/n-C 18 pairs normally used to determine the state of evolution of ails and of rock extracts. A calibration in terms of vitrinite reflectance is proposed.

  10. Case report: When measured free T4 and free T3 may be misleading. Interference with free thyroid hormones measurements on Roche® and Siemens® platforms

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    Lewandowski Krzysztof C


    Full Text Available Abstract A 59-year old female patient presented with apathy and 6 kg weight gain. Investigations revealed severe primary hypothyroidism (TSH>100 μIU/ml. L-thyroxine (L-T4 was started and titrated up to 75 μg, once daily, with clinical improvement. Other investigations revealed very high titres of anti-thyroid peroxidase (anti-TPO and anti-thyroglobulin (anti-Tg antibodies. After three months, there was a fall in TSH to 12.74 μIU/ml, however, with unexpectedly high free T4 (FT4 - 6.8 ng/ml and free T3 (FT3 - 6.7 pg/ml concentrations [reference range (rr: 0.8-1.9 ng/ml and 1.5-4.1 pg/ml (Siemens®, respectively]. At this stage L-T4 was stopped, and this was followed by a rapid increase in TSH (to 77.76 μIU/ml and some decrease in FT4 and FT3, however FT4 concentration remained elevated (2.1 ng/ml. Following this, L-T4 was restarted. On admission to our Department, she was clinically euthyroid on L-T4, 88 μg, once daily. Investigations on Roche® platform confirmed mildly elevated TSH - 5.14 (rr: 0.27-4.2 μIU/ml with high FT4 [4.59 (rr: 0.93-1.7 ng/ml] and FT3 [4.98 (rr: 2.6-4.4 pg/ml] concentrations. Other tests revealed hypoechogenic ultrasound pattern typical for Hashimoto thyroiditis. There was no discrepancy in calculated TSH value following TSH dilution (101% recovery. Concentrations of FT4 and FT3 were assessed on the day of discontinuation of L-T4 and after four days by the means of Abbott® Architect I 1000SR platform. These revealed FT4 and FT3 concentrations within the reference range [e.g., FT4 - 1.08 ng/ml (rr: 0.7-1.48] vs 4.59 ng/ml (rr: 0.93-1.7, Roche®, FT3 - 3.70 pg/ml (rr: 1.71-3.71 vs 4.98 (rr: 2.6-4.4, Roche®], confirming assay interference. Concentrations of ferritin and SHBG were normal. Conclusions Clinicians must be aware of possible assay interference, including the measurements of FT4 and FT3 in the differential diagnosis of abnormal results of thyroid function tests that do not fit the patient clinical

  11. Mechanical Properties of Rocks: Pore Pressure and Scale Effects Propriétés mécaniques des roches : pression de pore et effets d'échelle

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    Gueguen Y.


    Full Text Available Pore pressure plays a major role when considering rocks mechanical properties. In that field, the concept of effective pressure is a key one to deal with fluids mechanical effects. However, its frequent use has been the source of frequent confusing statements. Because of the various meanings which have been attached to that concept, an attempt is made in this paper to clarify it and examine the validity of its various uses relative to rock mechanical behaviour or rock properties. At a macroscopic scale, thermodynamics provides a powerful tool to investigate this. Reversible or irreversible thermodynamics provide general relationships of great interest. But because real rocks are non homogeneous systems, a microscopic approach is also required in order to analyze the mechanical properties from a description of the small scale processes. The microscopic approach is complementary of the macroscopic thermodynamic one as it leads to the calculation of the effective properties of the medium. In this last approach, effective medium theory is a powerful tool. The effective properties as derived from the microscale can be nicely combined to thermodynamic relations to interpret pore fluid pressure effects and scale effects. The example of elastic properties of porous rocks is more specifically emphasized to illustrate this because of both its intrinsic interest and importance as far as applications are concerned. La pression de pore joue un rôle de première importance dans la considération des propriétés mécaniques des roches. Dans ce domaine, le concept de contrainte effective est essentiel pour aborder les effets mécaniques. Toutefois, son utilisation fréquente a conduit à de nombreuses affirmations trompeuses. Compte tenu des significations diverses accordées à ce concept, nous tentons ici de le clarifier et examinons le domaine d'application de ses divers emplois dans le cadre du comportement mécanique ou des propriétés des roches. À l

  12. Symposium international PARAM 2002 : Identification et détermination des paramètres des sols et des roches pour les calculs géotechniques, Paris, 2-3 septembre 2002



    Les comptes rendus du symposium international sur l'identification et la détermination des paramètres des sols et des roches pour les calculs géotechniques, qui s'est tenu à Paris (France) les 2 et 3 septembre 2002, contiennent 61 communications. Ces communications traitent de sujets très variés et reflètent la situation actuelle de la recherche et de la pratique pour ce qui concerne le choix des paramètres pour les calculs de géotechnique. Les communications ont été réparties en quatre thème...

  13. 罗氏 Co bas c701全自动生化分析仪性能评价%Performance evaluation of Roche Cobas c 701 fully automatic biochemical analyzer

    Institute of Scientific and Technical Information of China (English)

    邓小玲; 侯玉磊; 陈特; 毕小云


    Objective To assess the performance of Roche Cobas c701 fully automatic biochemical analyzer. Methods According to EP15‐A2 from Clinical and Laboratory Standards Institute, the electrolyte (potassium, sodi‐um and chloride) and covers all the wavelengths of nine projects (alanine transaminase, aspartate transaminase, alka‐line phosphatase, gamma‐glutamine transaminase, creatinine and urea nitrogen, glucose, total protein, three acyl glyc‐erin) were measured by Roche Cobas c701 analyzer and original reagents. The precisions and accuracies of all parame‐ters were verified. Results In the 2 levels of tested parameters, the standard deviation of repeatability (Sr )was ≤the manufacture′s standard deviation of repeatability (σr ), and the standard deviation of prescision (St ) was≤the manu‐facture′s standard prescision (σt ), the prescision was acceptable and similar to what the manufacter declared. Correla‐tions between theoretic value and actual value were good (regression coefficient was :0. 999 4-1. 000 0). The bias of all parameters was acceptable (within the prescribed scope of CLIA′88)with Roche cobas c701analyzer, compared with the external quality assessment of the ministry of health clinical inspection center. Conclusion The repeatabili‐ty, precision and accuracy of the parameters by Roche Cobas c701 reach the performance that the manufacturer de‐clares.%目的:对罗氏Cobas c701全自动生化分析仪进行性能评价。方法按照美国临床和实验室标准化协会EP15‐A2文件的要求,通过电解质(钾、钠、氯)和涵盖各波长的9个项目(丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、碱性磷酸酶、γ‐谷氨酰转移酶、肌酐、尿素氮、葡萄糖、总蛋白、三酰甘油)对仪器的精密度、准确度、线性范围等进行验证。结果所有检测项目的重复性标准差(Sr)≤厂家声明的标准差(σr)、精密度的标准差(St)≤σt ,均

  14. The Cretaceous Source Rocks in the Zagros Foothills of Iran Les roches mères Crétacé de l'avant-pays du Zagros en Iran : un exemple de bassin intracratonique de grande dimension

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    Bordenave M. L.


    Zagros, constituée par les 40 000 km2 du Dezful Embayment , une zone déprimée située dans la province du Khuzistan. Les accumulations d'huile sont associées à un système pétrolier d'âge Crétacé/Tertiaire comprenant six niveaux roches mères d'importance inégale : la partie inférieure de la formation Garau (Néocomien et les formations Gadvan (Barrémien, Ahmadi (Cénomanien inférieur, Gurpi (Sénonien et Pabdeh (Eocène. Ces intervalles sont à l'origine de 99 % des réserves d'huile découvertes à terre en Iran et produites dans deux réservoirs principaux : les calcaires de l'Asmari (Miocène inférieur et du Bangestan (Cénomanien. Ces réservoirs contiennent 330 milliards de barils en places, soit plus de 7 % des réserves mondiales actuelles. Cet article aborde le problème de la distribution des roches mères de l'avant pays iranien et de la partie iranienne du golfe Arabo-Persique. Les facteurs géologiques, océanologiques et climatiques favorisant la mise en place des conditions dysaérobiques et anaérobiques et le dépôt de sédiments riches en matière organique sont discutés. Le rôle de chacune de ces roches mères dans l'approvisionnement des accumulations d'huile est rapidement évoqué. Deux des roches mères Crétacé : la formation Garau déposée au Néocomien dans la région du Luristan, du nord-est du Khuzistan et de la partie nord-est du golfe Arabo-Persique et la formation Kazhdumi, déposée à l'Albien dans la partie centrale du Dezful Embayment, présentent un excellent potentiel pétrolier avec des valeurs initiales (avant maturation thermique de carbone organique pouvant parfois dépasser 10%, des Index d'Hydrogène (IH de l'ordre de 600 mg HC/g C et une épaisseur maximum supérieure à 300 m. La matière organique sédimentaire, d'origine algaire, contient de fortes teneurs en soufre, ce qui la qualifie comme un kérogène de type IIS. Ces roches mères se sont déposées dans les dépressions bien marquées faisant partie

  15. Une méthode précise pour la mise en évidence et l'étude de l'anisotropie dans les roches An Accurate Method for Detecting and Analyzing Anisotropy in Rocks

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    Talebi S.


    Full Text Available L'objectif essentiel de cette recherche était de mettre au point une méthode expérimentale pour étudier l'anisotropie de la vitesse des ondes longitudinales (P dans les roches. Cette anisotropie aurait deux causes principales : d'une part, l'existence d'un réseau de pores, de fissures ou de microfissures, distribué de façon anisotrope dans la roche et d'autre part, la structure elle-même, dont l'anisotropie résulte de la nature des minéraux et de leur disposition. Dans un premier temps, nous avons commencé les essais sur des échantillons de carotte, forés dans trois directions perpendiculaires X, Y et Z d'un bloc de deux types de grès : grès des Vosges et grès de Fontainebleau. La vitesse du son a été mesurée sur 3 à 4 sections de carotte avec des intervalles de 30° (6 directions. Elles se distribuent nettement selon une ellipse, appelée l'ellipse d'anisotropie. Ces ellipses ont été tracées par mini-ordinateur et ont permis de mettre en évidence l'état d'anisotropie et l'homogénéité des carottes. La méthode est tellement précise qu'une erreur de repérage des axes sur un échantillon (Y de grès des Vosges a été détectée et certifiée à l'aide de direction des micas. Nous avons effectué le même essai sur les mêmes échantillons saturés sous vide. Une tendance générale à l'augmentation des vitesses, la diminution du degré d'anisotropie (le rapport de Vmax/Vmin et le changement de position de l'axe d'anisotropie a été observée. La comparaison des résultats secs et saturés permet d'avoir une idée de la nature des vides et de leur rôle dans l'anisotropie globale. Dans l'étape suivante de l'étude, nous avons mis au point une méthode de mesure sur les cubes à faces tronquées (66 faces au maximum de différents types de roches (2 grès, 2 marbres, 1 andésite, 1 balsate, et 1 calcaire. Les valeurs de vitesses des ondes (P ont été mesurées et reportées sur un diagramme de Schmidt. Ensuite

  16. 罗氏2010免疫分析仪检测甲胎蛋白的性能验证%Analytical performance of roche 2010 immunoassay system in detecting AFP

    Institute of Scientific and Technical Information of China (English)

    王凡; 蒋红君


    目的 探讨罗氏2010免疫分析仪检测甲胎蛋白(AFP)的基本分析性能与质量要求的符合性.方法 参照相关的文件,分别对罗氏2010免疫分析仪检测AFP的精密度、准确度、临床可报告范围和参考区间进行验证.结果 测定均值为11.90 IU/ml的样本,验证后批内精密度为0.28 IU/ml,总精密度为0.45 IU/ml,测定均值为237.23 IU/ml的样本,验证后批内精密度为2.85 IU/ml,总精密度为3.50 IU/ml,均与厂家声明一致;高、低浓度定值质控品检测结果与靶值的相对偏差均小于5%,5份室间质评控制品检测结果与靶值的相对偏倚为-0.41%~6.61%;分析测量范围为0.514~992.7 IU/ml,临床可报告范围为0.514~158 832 IU/ml;参考区间与厂家声明一致.结论 罗氏2010电化学发光免疫分析仪测定AFP的精密度、准确度、分析测量范围性能均与厂家声明一致;此验证方案和实验方法简便易行,可用于其他检验项目的性能验证.%OBJECTIVE To validate the basic performance of Roche 2010 Immunoassay System in detecting fetoprotein (AFP). METHODS The precision, accuracy, reportable range and reference interval of the Roche 2012 Immunoassay System in detecting AFP were analyzed. RESULTS The results of within-run precisions were 0.28 IU/ml of 11.90 IU/ml AFP level and 2.85 IU/ml of 237.23 IU/ml AFP level. The total precisions were 0.45 IU/ml of 11.90 IU/ml AFP level and 3.50 IU/ml of 237.23 IU/ml AFP level. There results were consistent with manufacturer's instruction; The relative bias between the measured results and the target values was less than 5%, and the relative bias between the measured results and the EQA control samples at five levels was -0.41%-6.61%; measurement range was 0.514-992.7 IU/ml within manufacturer's liner range. Clinical re-portable range was 0.514-158 832 IU/ml; Reference interval was 0.0-5.8IU/ml, which was consistent with manufacturer's instruction. CONCLUSION The Roche 2010

  17. Performance verification of Roche COBAS6000 automatic electrochemiluminescence immunoassay analyzer%罗氏COBAS6000全自动电化学发光免疫分析仪性能验证

    Institute of Scientific and Technical Information of China (English)

    谭晓辉; 王勇


    目的 按ISO15189要求对罗氏COBAS 6000全自动电化学发光免疫分析仪的性能进行验证. 方法 对甲胎蛋白(alpha-fetoprotein,AFP)的精密度、准确度、临床可报告范围(clinical reportable range,CRR)、分析测量范围(analytical measurement range,AMR)、参考区间进行验证实验. 结果 批内精密度变异系数(coefficient variation,CV)高低值分别为3.28%和3.46%;日间精密度CV高低值分别为4.39%和5.13%,均小于厂家提供的CV(10%).相对偏差为0.862%,小于5%.分析测量范围为0.80-1 200 ng/ml,参考区间为0-20.00 ng/ml,临床可报告范围为0-60 000 ng/ml. 结论 罗氏COBAS 6000全自动电化学发光免疫分析仪的性能与厂家提供的资料基本一致,故可用其进行临床标本的检验工作,所得结果具有可信性.%Objeaive To test and verify the system performance of Roche COBAS 6000 automatic electrochemiluminescence immunoassay analyzer according to the requirements of IS015189. Methods Verification experiments were taken to measure the precision.accuracy , clinical reportable range( CRR) , analytical measurement range ( AMR ) , reference interval of alpha-feloprotein ( AFP) . Re-sults The high and low values of coefficient variation( CV) of inter-assay were 3. 28% and 3. 46% , and those of between-day precision were 4. 39% and 5. 13% , which were all less than the CV provided by the manufacturer( 1O% ) . Relative bias was 0. 862% . Analytical measurement range was 0.80 - 1 200 ng/ml.the reference interval was O - 20. 00 ng/ml, and the clinical reportable range was O 60 000 ng/ml. Conclusion The basic performances of Roche COBAS6000 automatic electrochemiluminescence immunoassay analyzer are consistent wich the data provided by the manufacturer,so it can be used to inspect the clinical samples and the results are credible.

  18. Evaluation of Roche Cobas E601 for Determining CEA%罗氏Cobas E601检测癌胚抗原的方法学性能评价

    Institute of Scientific and Technical Information of China (English)

    苏维; 王淑仙; 陈占良; 冯惠清; 段琳; 李逸阳


    目的:对罗氏Cobas E601全自动电化学发光免疫分析仪检测癌胚抗原(CEA)的分析性能进行验证。方法对CEA的精密度、准确度、测量线性范围、参考区间和交叉污染率进行验证实验。结果批内精密度高低两种浓度的(CV)分别为4.96%和4.38%,日间精密度高低两种浓度的CV分别为4.99%和4.81%;5份室间质控品的检测结果与靶值的偏倚在1.49%~3.57%;测量线性范围与厂家提供的范围相近;CEA的测量数值有96.3%在提供的参考区间内;交叉污染率为0.12%。结论罗氏Cobas E601检测CEA的方法学性能良好,检验结果准确可靠,能够满足临床检测的要求。%Objective To evaluate the effects of Roche Cobas E601 automatic electrochemiluminescence immunoassay analyzer for determining CEA. Methods To analyze E601’s measurement precision, accuracy, measurement range, reference interval and carryover rate of CEA, verification experiments were taken to measure. Results The high and low values of CV of inter-assay were 4.96%and 4.38%, and between-day CV were 4.99% and 4.81%; 5 quality-control serum’s relative bias were between 1.49%-3.57%; there was good linear relationship between measured values and expected values;there was 96.3%of the measurement data in the recommended reference interval;carryover rate range was 0.12%. Conclusion Roche Cobas E601 analyzer is stable, precise and accurate.

  19. [Comparison between the centrifugation on MPA C10 (Roche Diagnostics) and the centrifugation according recommendations of GEHT (Groupe d'étude de l'hémostase et de la thrombose) for the daily hemostasis assays]. (United States)

    Flamant, Fabrice; Borg, Jeanne-Yvonne; Lenormand, Bernard; Le Cam-Duchez, Véronique


    Actually, many laboratories tend to acquire pre analytic automates to prepare specimens for analysis. For haemostasis, these pre analytical modules are not always in agreement with the recommendations from the Groupe d'étude de l'hémostase et de la thrombose (GEHT). For example in the MPA C10 module (Roche Diagnostics) the speed of centrifugation was not rather fast compared with the GEHT recommandations. Then, to be able to use this automate for routine coagulation assays, we compared results of Quick time, activated partial prothombin time, fibrinogen, factor II, factor V, factor VII, factor X and antithrombin levels and unfractioned heparin anti-Xa activity measurement after MPA (1,885 g - 999 sec) or GEHT (2,500 g - 900 sec) protocol of centrifugation. First, we verified platelet counts: in 82% of specimens, the platelet counts were under 10.10(9)/L after centrifugation on MPA module. Moreover, a good correlation was observed in all comparisons. Then we concluded the MPA C10 module was usable for routine coagulation tests.

  20. Application of paper chromatography to the determination of some elements in minerals and rocks; Application de la chromatographie sur papier a la determination de certains elements dans les mineraux et les roches

    Energy Technology Data Exchange (ETDEWEB)

    Agrinier, H. [Commissariat a l' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires


    This publication describes our work in applying paper chromatography to the fields of mineralogy and geology. We have studied the separation and determination of the following elements: Nb, Ta, Ti, Co, Cu, Mn, Sn, Ge, As, Sb, Bi, U, Th, Be, Au, Pb, Zn, V and Mo. The development of separation methods for these various elements has led us to solve many interference problems. These methods make it possible to separate and measure mineral components. They also enable us to search for trace elements in ores, rocks soils and carbonaceous materials. Finally let us point out that this technique may equally be used for analysis in very radioactive environment. (author) [French] Dans cette etude, nous etendons le champ d'application de la chromatographie sur papier aux domaines de la mineralogie et de la geologie. Nous avons etudie la separation et la determination des elements suivants: Nb, Ta, Ti, Co, Cu, Mn, Sn, Ge, As, Sb, Bi, U, Th, Be, Au, Pb, Zn, V et Mo. La mise au point des methodes de separation concernant ces differents elements nous a amenes a resoudre de nombreux problemes d'interference. Ces methodes permettent la separation et le dosage des constituants des mineraux, ainsi que la recherche d'elements en traces dans les minerais, les roches, les sols et les materiaux carbonnes. Signalons pour terminer que cette technique peut egalement trouver son utilisation dans le domaine de l'analyse en milieu tres radio-actif. (auteur)

  1. Etude de la distribution des terres rares et des autres éléments en traces dans les roches carbonatées du district minier de Bou-Azzer-Bleïda (Anti-Atlas, Maroc) : conséquences génétiques et variations à l'approche des minéralisations


    Zouita, Fouzia


    Différents types de roches carbonatées se trouvent dans les formations géologiques précambriennes du district minier de Bou-Azzer-Bleïda. Il s'agit de filons hydrothermaux ou de niveaux sédimentaires. L'utilisation de différentes méthodes d'analyses (dont la Spectrométrie de Masse à étincelles) permet de dresser l'inventaire des éléments majeurs et en traces y compris les Terres Rares dans ces différentes roches carbonatées. L'étude particulière de la répartition des Terres Rares dans ces mat...

  2. La difícil precisión de la noción de establecimiento permanente a través de la cláusula del agente dependiente (STS de 12 de enero de 2012, Caso Roche)


    Ribes Ribes, Aurora


    La autora examina críticamente la Sentencia del Tribunal Supremo español de 12 de enero de 2012, recaída al hilo del Caso Roche, en relación con la determinanción de la existencia o no de establecimiento permanente a efectos fiscales, a través de la precisión de la cláusula del agente dependiente.

  3. Grouped frequent sequential patterns derived from terrestrial image time series to monitor landslide behaviour - Application to the dynamics of the Sanières/Roche Plombée rockslide. (United States)

    Péricault, Youen; Pothier, Catherine; Méger, Nicolas; Trouvé, Emmanuel; Vernier, Flavien; Rigotti, Christophe; Malet, Jean-Philippe


    Image time series acquired with remote sensing methods based on optical terrestrial photogrammetry have great potential for understanding and monitoring the Earth surface dynamics at local scale, and are particularly interesting for landslide monitoring. Image correlation techniques can be applied to calculate the displacement fields, in either the image geometry or the terrain geometry if orthorectification procedures are applied. The resulting products are times series of displacement vectors for each epoch in which knowledge extraction techniques can be applied to discover relevant movement patterns in space and time. We used an unsupervised method (Grouped Frequent Sequential patterns / GFS-patterns) based on the mining of the displacement field. The method was originally developed for the analysis of time series of satellite images. It involves the extraction of trends / sub-trends affecting each pixel covering at least a minimum surface area and sufficiently connected to each other. The results of the mining are presented in spatio-temporal location maps (STL-map) of each GFS-pattern. In these maps, the spatial information is given by the pixel locations and the time information is displayed using a color ramp. The method is tested on a time series of 36 optical terrestrial images of the Sanières/Roche Plombée rockslide (South East French Alps) from 28 of July to 1 September 2014. From this series 35 2D displacement fields were calculated for epochs of three days, and the time series of vector magnitude and direction were analysed with GFS-patterns / STL-map. The method allowed identifying several patterns corresponding to different kinematical behaviour of the rockslide (long-term creep at the top of the slope, surficial movement of the debris at the base of the slope). The unsupervised knowledge extraction method GFS-pattern / STL-map, originally developed to analyse time series of satellite images showed in this study real possibilities of use for

  4. Observations sur les microfaciès des roches sédimentaires prélevées sur la marge armoricaine Observations on the Microfacies of Sedimentary Rock Samples from the Armorican Margin

    Directory of Open Access Journals (Sweden)

    Pastouret L.


    Full Text Available L'étude des dragages de roches et de sédiments effectués sur la marge armoricaine entre 47 et 48, de latitude Nord permet d'en préciser l'évolution paléogéographique et tectonique et de l'intégrer à celle du golfe de Gascogne. L'analyse de mi crofaciès des échantillons rocheux dont l'âge varie du Jurassique supérieur au Néogène met en évidence - d'une part, l'appartenance de la bordure nord de la marge armoricaine au domaine mésogéen au moins jusqu'au Crétacé moyen; - d'autre part, la permanence d'un régime de plate-forme carbonatée qui paraît fonctionner du Tithonique au Tertiaire inférieur, sur laquelle ont pu s'installer des édifices récifaux notamment au Crétacé moyen. Pendant toute cette période la sédimentation a compensé la subsidence; - enfin, l'accentuation de la subsidence à partir de l'Éocène (accompagnée d'une diminution de la production de calcium $ qui est vraisemblablement en relation avec les phénomènes tectoniques majeurs qui ont affecté à ce moment la bordure sud de la plaque européenne. A study of rock samples and sedimenis obtained by dredging from the Armorican Margin (47 ta 48° N brings new data on the paleogeographic and tectonic evolution of this ares in relation ta the formation of the Bay of Biscay. Microfacies ranging from the Upper Jurassic ta the Neogene show - the Armorican Margin until al least the Middle Cretaceous belongs to the Mesogean realm ; - carbonate platform regime prevails from the Tithonian to the Lower Tertiory with reef building occurring in the Middle Cretaceous. During this whole period in the area under consideration, sedimentation accounts for or even exceeds subsidence ; - the subsidence rate increases (and/or carbonate production decreases toward the end of the Eocene, and this may be related ta first order tectonic events offecting the southern part of the European plate.

  5. Observations on the Primary Migration of Oil in the la Luna Source Rocks of the Maracaibo Basin, Venezuela Observations de la migration primaire de l'huile dans les roches mères de la Formation de La Luna dans le bassin de Maracaïbo, Venezuela

    Directory of Open Access Journals (Sweden)

    Talukdar S.


    Full Text Available In the present work, an integrated approach has been made to understand the primary migration of oil in the organic-rich, oil prone, predominantly carbonate source rocks of the La Luna Formation of the Maracaibo Basin. Geochemical data on source rock extracts and the simulation of oil generation by hydrous-pyrolysis experiments indicate that the primary migration and expulsion of oil began at an early stage of thermal maturity and that the bulk oil expulsion at peak generation could have been as high as 75%. Fluorescence and transmitted light petrography of the source rocks at different maturity levels show the dominant mode of oil expulsion through microfractures. It is interpreted from the geochemical and geological data that the primary migration of oil in the La Luna source rocks took place as a separate oil phase through microfractures caused by the excess fluid pressure due to hydrocarbon generation. The oils expelled from the La Luna source are enriched in saturated hydrocarbons and depleted in resins + asphaltenes compared to the La Luna rock extracts. However the distributions of n-alkanes, in the oils and the rock extracts are very similar, which suggest that fractionation of the n-alkanes did not occur during the primary migration. In the Maracaibo Basin, updip lateral primary migration of oil within the La Luna source system seem to have occurred for a considerable distance. Local internal migration has been observed at a relatively early stage of maturity. Cette étude consiste en une approche intégrée cherchant à comprendre la migration primaire de l'huile dans les roches mères de la Formation de La Luna dans le bassin de Maracaïbo, riches en matière organique, génératrices d'huile et à dominante carbonatée. Les données géochimiques sur des extraits de roches mères et la simulation de genèse d'huile par des expériences de pyrolyse en présence d'eau indiquent que la migration primaire et l'expulsion de l'huile ont

  6. A multiplexed microfluidic PCR assay for sensitive and specific point-of-care detection of Chlamydia trachomatis.

    Directory of Open Access Journals (Sweden)

    Deborah Dean

    Full Text Available BACKGROUND: Chlamydia trachomatis (Ct is the most common cause of bacterial sexually transmitted diseases (STD worldwide. While commercial nucleic acid amplification tests (NAAT are available for Ct, none are rapid or inexpensive enough to be used at the point-of-care (POC. Towards the first Ct POC NAAT, we developed a microfluidic assay that simultaneously interrogates nine Ct loci in 20 minutes. METHODOLOGY AND PRINCIPAL FINDINGS: Endocervical samples were selected from 263 women at high risk for Ct STDs (∼35% prevalence. A head-to-head comparison was performed with the Roche-Amplicor NAAT. 129 (49.0% and 88 (33.5% samples were positive by multiplex and Amplicor assays, respectively. Sequencing resolved 71 discrepant samples, confirming 53 of 53 positive multiplex samples and 12 of 18 positive Amplicor samples. The sensitivity and specificity were 91.5% and 100%, and 62.4% and 95.9%, respectively, for multiplex and Amplicor assays. Positive and negative predictive values were 100% and 91%, and 94.1% and 68.6%, respectively. CONCLUSIONS: This is the first rapid multiplex approach to Ct detection, and the assay was also found to be superior to a commercial NAAT. In effect, nine simultaneous reactions significantly increased sensitivity and specificity. Our assay can potentially increase Ct detection in globally diverse clinical settings at the POC.

  7. Generation of Anomalous Internal Pressures in Source Rocks and Its Role in Driving Petroleum Migration La genèse de pressions internes anormales dans les roches mères et son rôle de poussée dans la migration du pétrole

    Directory of Open Access Journals (Sweden)

    Barker C.


    . La présence de gaz conduit à des comportements très différents et la pression de pores dans les roches mères inférieure à la normale à moins qu'il n'y ait une genèse suffisante de la matière organique en hydrocarbures pour la compenser. En l'absence d'une genèse continue (c'est-à-dire pour le méthane biogénique, le gaz se dissout régulièrement et les pressions chutent considérablement en dessous de la pression hydrostatique. A l'inverse, sur les élévations, les systèmes gazéifères ont tendance à la surpression et la pression peut atteindre la charge de fracturation de la roche. Le craquage thermique des bitumes qui restent dans une roche mère au cours de l'enfouissement va de pair avec une augmentation de volume et, dans un pore isolé, le système des pressions atteint des valeurs élevées. Il suffit qu'un pour cent des bitumes de type huile soit transformé en gaz pour que la pression atteigne la charge pour laquelle la fracturation de la roche est une conséquence inévitable.


    Institute of Scientific and Technical Information of China (English)

    江志奎; Albad,J


    近年来实验室诊断丙型肝炎病毒(HCV)PCR法已向标准化和“即可用”的方向发展。本试验用来自5个检测中心的2000多份标本对首台自动化扩增和检测HCV RNA的仪器COBAS在日常实验室工作中的性能进行了评估。结果表明,自动的COBAS法与AMPLICOR手工法同样准确,符合率达99.8%。PCR法与血清学方法的结果并不完全一致,因为抗HCV抗体的存在有可能表示隐性感染或既往感染。如果以抗HCV抗

  9. Evaluation of Cobas TaqMan MTB PCR for detection of Mycobacterium tuberculosis. (United States)

    Kim, Jeong Hyun; Kim, Young Jae; Ki, Chang-Seok; Kim, Ji-Youn; Lee, Nam Yong


    Nucleic acid-based amplification tests allow the rapid detection of Mycobacterium tuberculosis. Recently, a real-time PCR assay for M. tuberculosis complex, the Cobas TaqMan MTB test (Roche Diagnostics, Basel, Switzerland), was introduced. We performed a prospective study to evaluate the diagnostic performance of the Cobas TaqMan MTB test system. A total of 406 specimens collected from 247 patients were simultaneously tested by conventional culture, Cobas Amplicor MTB PCR, and TaqMan MTB PCR. The cross-reactivity with other Mycobacterium species and the detection limit were also evaluated. Among 406 specimens, a total of 24 specimens (5.9%) were culture positive: 14 specimens were positive by both TaqMan and Amplicor MTB PCRs, while 5 specimens were positive by only TaqMan PCR. The remaining five specimens were negative by both PCR methods. Seven specimens with negative culture results were positive by TaqMan PCR, but five of these were negative by Amplicor MTB PCR. The sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values were 79.1%, 98.2%, 73.1%, and 98.7% for TaqMan and 58.3%, 99.5%, 87.5%, and 97.4% for the Amplicor MTB PCR test, respectively. There was no cross-reactivity with M. tuberculosis and nontuberculous mycobacterial species. The detection limit for the Cobas TaqMan MTB PCR test was 4.0 copies/μl. The Cobas TaqMan MTB PCR test showed higher sensitivity for detection of the M. tuberculosis complex without disturbing the specificity and NPV than the Amplicor MTB PCR test.

  10. 罗氏生化多项定标液(CFAS)在 ADVIA1800自动生化仪上的应用%Roche biochemical multiple calibration solution (CFAS)used in the ADVIA 1800 automatic biochemical discussion on

    Institute of Scientific and Technical Information of China (English)



    目的:规范实验室生化项目的参考范围,统一临床检验数值,为临床医师提供更准确、更一致的检验结果,方便患者治疗效果的评价。方法同步于罗氏Cobas C501自动生化仪和ADVIA1800自动生化仪上进行多项目的评价。结果1.定标与校正验证合格;2.正常质控品和异常质控品在可控范围内;3.临床常规标本比对评价没有显著性差异。结论罗氏生化多项定标液(CFAS)在ADVIA1800自动生化仪上的应用效果满意,能为医师提供准确、统一的检验结果,方便医师治疗病人及疗效观察。%Objective to standardize laboratory biochemical reference scope of the project ,unified clinical inspection number , provide clinicians with more accurate and more consistent test results ,convenient patient treatment effect evaluation .Methods the synchronous in roche Cobas C501 automatic biochemistry analyzer and multiple objective evaluation ADVIA 1800 automatic bio‐chemistry analyzer .Results 1 ,the calibration and correction verification qualified ,2 ,normal quality products and quality control within the scope of the controllable ,3 ,there is no significant difference between clinical specimens of normal ratio evaluation .Con‐clusion roche biochemical multiple scaling liquid(CFAS)in the application effect of satisfaction on ADVIA 1800 automatic biochem‐istry analyzer ,for physicians to provide accurate ,unified inspection result ,convenient for doctors treat patients and curative effect observation .

  11. The method of evaluation and establishment of reference intervals of serum iron in the Roche ModularDDP automatic biochemical analyzer%罗氏ModularDDP全自动生化分析仪检测血清铁的方法学评价及参考区间建立

    Institute of Scientific and Technical Information of China (English)

    廖君群; 毕小云


    Objective To evaluate the serum iron(SI) detection method in the Roche ModularDDP automatic biochemical analyzer,and establish the laboratory reference range. Methods We collected 240 samples of healthy people,detected SI with FerroZine spectrophotometry and established the laboratory reference interval. According to American Society for Clinical Laboratory Standards Committee EP documents, the detection of SI was evaluated with the precision, accuracy, linearity, detection limit (detection limit) and interference. Results The accuracy of SI was below 2%, the accuracy was between 97. 93% and 103. 48%,with minimum detection limit as 0. 28 μmol/L,the linearity was from 0. 95 μmol/L to 184. 40 μmol/L. Hemoglobin((第3版)血清铁测定的参考区间有明显差异,说明该参考区间不可直接引用,也说明了进行方法学评价的重要性.

  12. Evaluation of Abbott RealTime CT/NG assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in cervical swabs from female sex workers in China.

    Directory of Open Access Journals (Sweden)

    Yan Han

    Full Text Available BACKGROUND: To evaluate the performance of the Abbott RealTime CT/NG assay for detection of Chlamydia trachomatis (CT and Neisseria gonorrhoeae (NG among female sex workers (FSWs in China. METHODS: Cervical swabs from 997 participants were blindly detected by the Abbott RealTime CT/NG assay on the automated m2000 molecular platform and Roche Cobas Amplicor CT/NG assay. Discrepant analysis were confirmed by the Qiagen care CT PCR assay. The sample was defined as candidate nvCT-positive if it was CT positive in the Abbott m2000 assay, but CT negative in the other two assays. RESULTS: 25 specimens that were discordant for CT and 26 specimens that were discordant for NG between the two assays were resolved by Qiagen care CT & NG PCR assays. The sensitivity and specificity, respectively, for Abbott m2000 assay were 92.59% and 100% for CT and 95.45% and 99.90% for NG. The positive predictive value (PPV and negative predictive value (NPV of Abbott m2000 assay were100% and 98.52% for CT and 95.5% and 99.90% for NG, respectively. No candidate new-variant CT(nvCTspecimens were identified. CONCLUSION: Abbott RealTime CT/NG assay were more specify for CT and NG detection, however, its sensitivity for CT and NG were a little bit lower than Roche Cobas Amplicor CT/NG assay. Abbott RealTime CT/NG assay had higher PPV for NG detection than Roche Cobas Amplicor CT/NG assay; it would be more suitable for screening for population with low-prevalence NG. There is currently no evidence that nvCT is present in FSWs in China.

  13. Étude de la mouillabilité des roches réservoir à l'échelle du pore par cryomicroscopie électronique à balayage Wettability of Reservoir Rock At the Pore Scale: Contribution of Cryo-Scanning Electron Microscopy

    Directory of Open Access Journals (Sweden)

    Fassi-Fihri O.


    Full Text Available Le but de cette étude est de caractériser, à l'échelle du pore, la mouillabilité des roches réservoir, en relation avec leur géométrie et/ou leur minéralogie. Cette caractérisation se fait, après congélation des échantillons, par l'observation de la distribution des fluides au sein du milieu poreux (saumure et huile brute, en microscopie électronique à balayage. Les expériences ont d'abord été effectuées sur des roches modèles parfaitement mouillables à l'eau, verre fritté et grès naturels. Certains de ces minéraux ont été rendus hydrophobes par greffage de silane. L'étude de ces systèmes a mis en évidence une corrélation entre la mouillabilité et la distribution des fluides. Puis, une roche réservoir (grès argileux de la formation de Brent, de mer du Nord connue comme étant de mouillabilité intermédiaire a été étudiée. Un travail précédent (étude de déplacements eau/huile par tomographie X avait abouti à la conclusion que si ces roches présentaient des hétérogénéités de mouillabilité, l'échelle de ces hétérogénéités devait être inférieure au millimètre. Les études de cryomicroscopie ont montré le caractère hydrophobe de la kaolinite, tandis que les illites, le quartz et les feldspaths sont préférentiellement mouillables à l'eau. L'imbibition spontanée d'huile pourrait ainsi être attribuée à l'existence au sein de la roche d'un réseau de kaolinite, tandis que l'imbibition spontanée de saumure serait due à l'existence d'un second réseau plus ou moins imbriqué avec le premier et constitué des autres minéraux. Un autre cas de roche réservoir a été étudié, à savoir un carbonate du Moyen-Orient. Les mésopores intergranulaires y ont été observés comme étant mouillables à l'huile tandis que les micropores restaient mouillables à l'eau. Dans ce cas, la mouillabilité intermédiaire de ces échantillons s'explique par la géométrie plutôt que par la min

  14. Comparison of the Roche RealTime ready Influenza A/H1N1 Detection Set with CDC A/H1N1pdm09 RT-PCR on samples from three hospitals in Ho Chi Minh City, Vietnam (United States)

    Tham, Nguyen thi; Hang, Vu thi Ty; Khanh, Trong Huu; Viet, Do Chau; Hien, Tran Tinh; Farrar, Jeremy; van Vinh Chau, Nguyen; van Doorn, H. Rogier


    Background Real-time PCR can be considered the gold standard for detection of influenza viruses due to its high sensitivity and specificity. Roche has developed the RealTime ready Influenza A/H1N1 Detection Set, consisting of a generic influenza virus A PCR targeting the M2 gene (M2 PCR) and a specific PCR targeting the HA of A/H1N1-pdm09 (HA PCR, 2009 H1N1), with the intention to make a reliable, rapid, and simple test to detect and quantify 2009 H1N1 in clinical samples. Methods We evaluated this kit against the USCDC/WHO real-time PCR for influenza virus using 419 nose and throat swabs from 210 patients collected in 3 large hospitals in Ho Chi Minh city, Vietnam. Results In the per patient analysis, when compared to CDC PCR, the sensitivity and specificity of the M2 PCR were 85.8 and 97.6%, respectively; the sensitivity and specificity of HA PCR were 88.2 and 100%, respectively. In the per sample analysis, the sensitivity and specificity in nose swabs were higher than in throat swabs for both M2 and HA PCRs. The viral loads as determined with the M2 and HA PCRs correlated well with the Ct values of the CDC PCR. Conclusion Compared with the CDC PCR, the kit has a reasonable sensitivity and very good specificity for the detection and quantification of Influenza A virus and A/H1N1-pdm09. However, given the current status of 2009 H1N1, a kit that can detect all circulating seasonal influenza viruses would be preferable. PMID:22785431

  15. A Riemann-Roch Theoremfor One-Dimensional Complex Groupoids (United States)

    Perrot, Denis

    We consider a smooth groupoid of the form Σ⋊Γ, where Σ is a Riemann surface and Γ a discrete pseudogroup acting on Σ by local conformal diffeomorphisms. After defining a K-cycle on the crossed product C0(Σ)⋊Γ generalising the classical Dolbeault complex, we compute its Chern character in cyclic cohomology, using the index theorem of Connes and Moscovici. This involves in particular a generalisation of the Euler class constructed from the modular automorphism group of the von Neumann algebra L∞(Σ)⋊Γ.

  16. Mida roheline tarbija tahab / Catherine Roche ; intervjueerinud Michael S. Hopkins

    Index Scriptorium Estoniae

    Roche, Catherine, 1977-


    2008. a. tehtud uuringust, mille abil selgitati välja, mis põhjustel inimesed eelistavad tarbimisel rohelist tootevalikut. Skeemid: Valmisolek maksta rohelise eest rohkem sõltub tootekategooriast ja tajutavatest eelistest; Teadlikkus ja valik

  17. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.

    number of points for curves of their respective genera. Linear network coding transmits information in terms of a basis of a vector space and the information is received as a basis of a possibly altered vector space. Ralf Koetter and Frank R. Kschischang %\\cite{DBLP:journals/tit/KoetterK08} introduced...

  18. Riemann-Roch Spaces and Linear Network Codes

    DEFF Research Database (Denmark)

    Hansen, Johan P.


    number of points for curves of their respective genera. Linear network coding transmits information in terms of a basis of a vector space and the information is received as a basis of a possibly altered vector space. Ralf Koetter and Frank R. Kschischang %\\cite{DBLP:journals/tit/KoetterK08} introduced...

  19. Clinical Performance of Roche Cobas 4800 HPV Test (United States)

    Cui, Miao; Chan, Nicholas; Liu, Momo; Thai, Khanh; Malaczynska, Joanna; Singh, Ila; Zhang, David


    Evaluation of the Cobas 4800 test demonstrated that Cobas had a low rate of cross-reactivity with low-risk human papillomavirus (lrHPV), a 3.74% disconcordance rate between prealiquots and postaliquots, and failure rates of 4.57% and 1.16%, respectively, after vortexing and swirling. This study demonstrated that the Cobas test has good sensitivity, accuracy, and reproducibility for detecting 14 high-risk HPV (hrHPV) genotypes. PMID:24719443

  20. In the heart of rock; Au coeur de la roche

    Energy Technology Data Exchange (ETDEWEB)



    This educational document gives a presentation of the Andra underground research laboratory of Bure (Haute-Marne and Meuse, France) devoted to the study of the underground disposal of radioactive wastes. The document is presented in the form of a comics. It describes the geological setting of the laboratory, located in the eastern edge of the Paris basin, the properties of the Callovian-Oxfordian clay layer, the digging of the boreholes and galleries, the mine-by test and its instrumentation, the backfilling and sealing of the galleries and the reversibility of the burial of radioactive waste containers. (J.S.)

  1. Duplex detection of the Mycobacterium tuberculosis complex and medically important non-tuberculosis mycobacteria by real-time PCR based on the rnpB gene. (United States)

    Abdeldaim, Guma; Svensson, Erik; Blomberg, Jonas; Herrmann, Björn


    A duplex real-time PCR based on the rnpB gene was developed for Mycobacterium spp. The assay was specific for the Mycobacterium tuberculosis complex (MTB) and also detected all 19 tested species of non-tuberculous mycobacteria (NTM). The assay was evaluated on 404 clinical samples: 290 respiratory samples and 114 from tissue and other non-respiratory body sites. M. tuberculosis was detected by culture in 40 samples and in 30 samples by the assay. The MTB assay showed a sensitivity similar to Roche Cobas Amplicor MTB-PCR (Roche Molecular Systems, Pleasanton, CA, USA). There were only nine samples with non-tuberculous mycobacteria detected by culture. Six of them were detected by the PCR assay.

  2. Proposition d'explication de la formation d'hydrogène sulfuré dans les stockages souterrains de gaz naturel par réduction des sulfures minéraux de la roche magasin Proposed Explanation of Hydrogen-Sulfide Formation in Underground Natural-Gas Storage Structures by Reduction of Mineral Sulfides in the Reservoir Rock.

    Directory of Open Access Journals (Sweden)

    Bourgeois J. P.


    Full Text Available La formation d'hydrogène sulfuré dans les structures de stockage peu expliquer autrement que par l'action de bactéries sulfato-réductrices. La contenue dans la roche magasin constitue une source de sulfures capable d'alimenter en H2S le gaz naturel. La réduction de la pyrite en sulfures du type Fe 1-x S et l'équilibre de dissolution précipitation, lié principalement à la pression de CO2, dans les structures stockages, constituent un processus de formation d'H2S capable d'expliquer tativement et quantitativement les phénomènes observés sur le terrain. Un modèle simplifié de stockage reprend ce schéma et teste la sensibililté de la teneur en H2S à la valeur des paramètres physiques et chimiques définissant le stockage. Cette étude permet de proposer un certain nombre d'actions susceptibles de limiter la formation d'H2S et d'orienter les choix futurs du couple gaz naturel - structures de stockage. The formation of hydrogen sulfide in storage structures can be explained otherwise thon by the action of sulfate-reducing bacteria. The pyrite contained in the reservoir rock makes up a source of sulfides capable of supplying the natural gas with H2S.Reduction of pyrite ta sulfides of the Fe,-,S type and the dissolution precipitation equilibrium, linked mainly ta C02 pressure in storage structures, make up an H2S for-mation process capable of qualitatively and quantitatively explained phenomena observed in the field.A simplified storage model reflects this scheme and can be used ta test the sensi-tivity of the H2S content ta the value of the physical and chemical parameters defining the storage structure.This investigation can be used to propose various means of action (sable ta "mit H2S formation and ta guide future choices of natural gas/storage-structure pairs.

  3. Le souffre thiophénique dans les pétroles et les extraits de roche. Analyse par spectrométrie de masse et chromatographie en phase gazeuse Mass-Spectrometry and Gas-Chromatography Analysis of Thiophene Sulfur in Oil and Rock Extracts

    Directory of Open Access Journals (Sweden)

    Castex H.


    Full Text Available On étudie par spectrométrie de masse à haute résolution la répartition du soufre thiophénique par coupe de distillation et par nombre de carbone dans la fraction > C10 d'un pétrole brut. Parallèlement on chromatographie à l'aide d'un détecteur sélectif à photométrie de flamme les diverses fractions thiophéniques. L'examen des chromatogrammes permet de déterminer les zones dé réponse et l'allure générale des différentes coupes thiophéniques. Les applications sont variées : on vérifie le bien-fondé de l'utilisation d'un spectromètre de masse à basse résolution pour les analyses en routine d'huiles brutes et on peut, par la connaissance des différents profils de type thiophénique prévoir des analogies ou des différences au sein d'huiles brutes et d'extraits de roche d'un même bassin pétrolier, ce qui limite le nombre d'échantillons à analyser en détail. High-resolution mass spectrometry is used to analyze the distribution of thiophenic sulfur per distillation cut and per carbon number in the > C10 fraction of a crude oil. At the same time, a selective flame-photometry sensor is used to chromatograph the different thiophenic fractions. An analysis of the chromatograms serves to determine the response zones and the general aspect of the different thiophenic cuts. There are various applications of this process. The validity of using a low-resolution mass spectrometer is checked for routine crude-cil analyses. By determining the different profiles of the thiophenic type, similarities or differences can be predicted in the crude cils and rock extracts from the saure cil basin. This limits the number of samples having to be analyzed in detail.

  4. Potential applications of oral brush cytology with liquid-based technology: results from a cohort of normal oral mucosa. (United States)

    Kujan, Omar; Desai, Mina; Sargent, Alexandra; Bailey, Andrew; Turner, Andrew; Sloan, Philip


    Fifty healthy volunteers were studied to assess the potential applications of oral brush sampling using liquid-based cytology. Three specimens from the buccal mucosa and lateral border of tongue were collected from each subject by using cervical brushes and brooms. The brush was immersed in a preservative fluid. The sample in the preservative fluid was processed according to the manufacturer's directions (SurePath, UK). Slides were stained by the Papanicolaou method and assessed for squamous cell adequacy by the same criteria used for cervical cytology screening. Immunocytochemical staining for FHIT (Fragile Histidine Triad) was applied in liquid-based preparations following the streptavidin-biotin-peroxidase method. Human papillomavirus (HPV) detection was performed using the Hybrid Capture 2 assay (Digene) and the PCR-based Roche AMPLICOR HPV Test. LBC preparation slides showed good sample preservation, specimen adequacy and visualization of cell morphology. Interestingly, nine cases showed borderline cytological abnormalities from apparently normal oral mucosa. All cases showed good quality positive FHIT immunoreactivity staining. All studied cases were high-risk HPV negative using HC2 assay method. However, the AMPLICOR Roche Test detected four samples with positive results for high-risk HPVs. Liquid-based cytology has potential as a screening tool for oral cancer and precancer. The method may also have applications for research and practice in the field of oral cancer and precancer. However a special custom-designed oral cytobrush is required.

  5. Approche numérique et quantitative de l'étude sur clichés aériens de la fracturation des réservoirs en roches fissurées Numerical and Quantitative Approach to Investigating Fracture Maps of Fissured Reservoirs from Airphotos

    Directory of Open Access Journals (Sweden)

    Razack M.


    Full Text Available Dans le cadre de la détermination de la structure des réservoirs en roches fissurées, une méthodologie de traitement numérique et quantitative de la fractu-ration relevée sur clichés aériens a été mise au point. La numérisation du relevé est obtenue par digitalisation sur un lecteur de courbes. Cette information est ensuite traitée à l'aide d'un algorithme (programme RAFRAC qui permet d'identifier numériquement la fracturation par un ensemble de para-mètres élémentaires (orientation, longueur , densité... à partir desquels il devient possible de procéder à une analyse approfondie du champ de fractures considéré. Un exemple d'application est présenté à propos de l'étude de la fracturation d'une zone à structure tabulaire (Causse du Larzac. Ces premiers résultats tendent à mettre en évidence une double nature mathématique de l'information analysée aléatoire d'une part, déterministe d'autre part. On montre également, à partir de la théorie desvariables régionalisées, que la fracturation suit une certaine logique dans sa répartition spatiale. Par ailleurs une organisation des familles de fractures a pu être mise en évidence, sur laquelle la structure géométrique du réservoir sous-jacent s'ajuste de façon très proche. As part of efforts ta determine the structure of reservoirs in fissured rocks, a numerical and quantitative processing methodology has been developed for mapping fractures with airphotos. The survey is digitized on a curve reader. This data is then processed by means of an algorithm (RAFRAC program sa as ta numerically identify fracturing by a set of elementary parameters (direction, length, density, etc. which con be used ta make an in-depth analysis of the fracture field being considered. A sample application is described having ta do with the surveying of the fracturing in a tabular structure (Causse plateau of Larzac. These initial results tend to reveol a dual mathematical nature

  6. Rock Permeability and Fluid Pressure At the Ktb. Implications from Laboratory-And Drill Hole-Measurements Perméabilité des roches et pression dans le KTB : enseignements tirés des mesures de laboratoire et des mesures en puits

    Directory of Open Access Journals (Sweden)

    Zimmermann G.


    Full Text Available Rock permeability and the fluid pressure were investigated at different scales at the two drill holes of the Continental Deep Drilling Program (KTB. Drill hole tests and fluid inclusion investigations both implicate the existence of hydrostatic fluid pressure in situ with respect to salinity of the formation fluid. Matrix permeability and in situ values from hydraulic tests differ up to three decades with higher values in situ. Further on, the pressure dependence of core permeability and in situ determined values differ significantly. All these observed effects support the well known theory of scale variance. This conclusion is supported by observations of hydraulic communications between both drill holes. These scale effects implicate a pronounced hydraulic heterogeneity of the KTB surroundings. Therefore, stochastic network modelling with parameters derived from structural borehole measurements and under the consideration of the observed permeabilities were performed. Under the presumption of existing driving forces fluid transport takes place dominantly on discrete connected pathways characterised by fracture width, fracture length and fracture orientation and is subordinate in the rock matrix. La perméabilité des roches et la pression des fluides ont été étudiées à différentes échelles sur les deux forages du Programme continental de forage profond - Continental Deep Drilling Program (KTB. Les essais de puits et les recherches d'inclusions de fluides impliquent l'existence d'une pression de fluide fonction de la salinité du fluide de formation. Les valeurs déduites des essais de puits dépassent largement les perméabilités matricielles, l'écart allant jusqu'à trois ordres de grandeur. De plus, l'évolution de la perméabilité mesurée sur échantillon en fonction de la pression et celle déterminée in situ diffèrent largement. Ces observations renforcent la théorie bien connue des effets d'échelle. Cette conclusion est

  7. Propagation des ondes élastiques dans les matériaux non linéaires Aperçu des résultats de laboratoire obtenus sur les roches et des applications possibles en géophysique Propagation of Elastic Waves in Nonlinear Materials Survey of Laboratory Results on Rock and Geophysical Applications

    Directory of Open Access Journals (Sweden)

    Rasolofosaon P.


    Full Text Available Les roches présentent souvent un comportement élastique nettement non linéaire, entraînant des conséquences importantes sur la propagation des ondes. Cette non-linéarité élastique est surtout causée par les microdéfauts mécaniques ubiquistes (microfissures, joints de grains, macles, etc. dont la rigidité varie sous l'effet de la contrainte. Ce sujet fait l'objet d'études de plus en plus nombreuses. Nous nous proposons de présenter très sommairement les bases théoriques et les résultats expérimentaux permettant d'avoir un ordre de grandeur des effets caractéristiques observés dans les roches afin de pouvoir proposer une approche critique des possibilités d'applications en géophysique. Deux disciplines se sont développées en parallèle à partir du même principe physique et avec des formalismes très proches : - L'acousto-élasticité étudie l'effet des précontraintes statiques sur les vitesses de propagation des ondes élastiques. On dispose d'un formalisme mécanique élaboré permettant de relier quantitativement variation de contrainte et variation de vitesse élastique (par exemple pour ce qui concerne l'anisotropie acoustique induite par un état de contrainte et d'une méthode expérimentale de mesure des coefficients de non-linéarité. - L'acoustique non linéaire s'intéresse aux conséquences de la variation des modules élastiques au passage d'une onde qui ne peut plus être considérée comme une petite perturbation, mais qui induit localement des modifications mesurables du milieu de propagation ; modifications entraînant l'apparition de phénomènes inconnus en acoustique linéaire tels que la génération d'harmoniques et l'interaction onde-onde. Les applications à la sismique pétrolière semblent fort lointaines puisque, avec les méthodes classiques de surface ou de puits, il y a peu d'espoir de réussir à faire propager jusqu'aux couches profondes des ondes dont l'amplitude dépasserait le seuil de

  8. Evolution of the Petrophysical and Mineralogical Properties of Two Reservoir Rocks Under Thermodynamic Conditions Relevant for CO2 Geological Storage at 3 km Depth Évolution des propriétés physiques et minéralogiques de deux roches réservoirs dans des conditions thermodynamiques correspondant à un stockage géologique de CO2 à 3 km de profondeur

    Directory of Open Access Journals (Sweden)

    Rimmelé G.


    est considérée comme une solution pour contribuer à la réduction des émissions de gaz à effet de serre dans l’atmosphère. Les interactions entre le CO2 supercritique et la roche-réservoir potentielle doivent être étudiées en détail en conditions de stockage géologique. Quarante échantillons de calcaire de Lavoux et de grès d’Adamswiller, provenant de roches réservoirs du bassin parisien, ont été expérimentalement exposés au CO2 dans un autoclave spécialement construit pour reproduire les conditions thermodynamiques d’un réservoir de stockage de CO2. Les deux types de roches ont été exposés pendant un mois à du CO2 supercritique humide et à de l’eau saturée en CO2, à 28 MPa et 90 °C, ce qui correspond à des conditions d’enfouissement de 3 km de profondeur. L’évolution de leurs propriétés minéralogiques a été suivie par des analyses par diffraction des rayons X, par spectroscopie Raman et par microscopie électronique couplée à un système de microanalyses X. Leurs propriétés physiques et microtexturales ont été estimées en mesurant, avant et après les expériences, le poids, la densité, les propriétés mécaniques, la perméabilité, la porosité globale et la présence d’éventuels gradients de porosité de chaque échantillon. Les résultats montrent que les deux roches ont préservé leurs propriétés mécaniques et minéralogiques, malgré une augmentation de la porosité et de la perméabilité. Des zones microscopiques de dissolution de la calcite observées dans le calcaire sont vraisemblablement responsables de cette augmentation de la perméabilité et de la porosité. Dans le grès, une altération de la pétro-fabrique est supposée se produire due à la réaction des minéraux argileux avec le CO2. Tous les échantillons du calcaire de Lavoux et du grès d’Adamswiller ont montré une altération mesurable dans le CO2 humide et dans l’eau chargée en CO2. Ces expériences en autoclave sont

  9. Impact of Diagenetic Alterations on the Petrophysical and Multiphase Flow Properties of Carbonate Rocks Using a Reactive Pore Network Modeling Approach Impact des altérations diagénétiques sur les propriétés pétrophysiques et d’écoulement polyphasique de roches carbonates en utilisant une modélisation par l’approche réseau de pores

    Directory of Open Access Journals (Sweden)

    Algive L.


    replacing the original complex pore structure of real porous media by a conceptual network. The second step consists of resolving the governing equations of the precipitation and dissolution phenomena (i.e. reactive convection diffusion equation in the conceptual 3D pore network and deducing the local reactive fluxes and the motion of the fluid-solid interface. The third step consists of updating the new pore structure and calculating the new petrophysical properties of the modified porous media. Those steps are repeated in order to mimic a given diagenetic scenario. Finally, the multiphase flow properties of the current porous media are calculated. The impact of one diagenetic cycle of dissolution and precipitation on the pore networks’ heterogeneity and consequently on the petrophysical properties (i.e. porosity and permeability and multiphase flow properties (i.e. relative permeability and capillary pressure have been investigated. The permeability and porosity evolution during a given diagenetic cycle are calculated and analyzed as a function of the relevant dimensionless numbers (Peclet and Damköhler numbers that characterize the flow and reaction regime. The correlation between these numbers and the dissolved/precipitated layer thickness distribution is investigated. This work contributes to improve the understanding of the impact of dissolution and precipitation on permeability and porosity modification. Using the PNM approach, multiphase flow properties and permeability-porosity relationship have been determined for different reactive flow regimes. These relationships are relevant input data to improve the quality of reservoir simulation predictions. Les roches sédimentaires présentent souvent une structure porale hétérogène qui est intrinsèquement liée à la texture de la roche d’origine et aux modifications diagénétiques subies. Ces altérations sont régies par la texture de la roche d’origine, les fluides impliqués (et les interactions rock

  10. Impact of HIV-1 genetic diversity on plasma HIV-1 RNA Quantification: usefulness of the Agence Nationale de Recherches sur le SIDA second-generation long terminal repeat-based real-time reverse transcriptase polymerase chain reaction test. (United States)

    Rouet, François; Chaix, Marie-Laure; Nerrienet, Eric; Ngo-Giang-Huong, Nicole; Plantier, Jean-Christophe; Burgard, Marianne; Peeters, Martine; Damond, Florence; Ekouevi, Didier Koumavi; Msellati, Philippe; Ferradini, Laurent; Rukobo, Sandra; Maréchal, Valérie; Schvachsa, Nilda; Wakrim, Lahcen; Rafalimanana, Christian; Rakotoambinina, Benjamin; Viard, Jean-Paul; Seigneurin, Jean-Marie; Rouzioux, Christine


    The high genetic diversity of HIV-1 has a major impact on the quantification of plasma HIV-1 RNA, representing an increasingly difficult challenge. A total of 898 plasma specimens positive for HIV-1 RNA by commercial assays (Amplicor v1.5; Roche Diagnostic Systems, Alameda, CA or Versant v3.0; Bayer Diagnostics, Emeryville, CA) were tested using the Agence Nationale de Recherches sur le SIDA second-generation (G2) real-time reverse transcriptase polymerase chain reaction (RT-PCR) test: 518 samples containing HIV-1 of known subtype, including 88 from 2 subtype panels and 430 harboring B (n = 266) and non-B (n = 164) group M HIV-1 subtypes from patients followed up in 2002 through 2005 at Necker Hospital (Paris, France), and 380 samples from 10 different countries (Argentina, Cambodia, Cameroon, Central African Republic, France, Ivory Coast, Madagascar, Morocco, Thailand, and Zimbabwe). HIV-1 RNA values obtained by G2 real-time PCR were highly correlated with those obtained by the Amplicor v1.5 for B and non-B subtypes (R = 0.892 and 0.892, respectively) and for samples from diverse countries (R = 0.867 and 0.893 for real-time PCR vs. Amplicor v1.5 and real-time PCR vs. Versant v3.0, respectively). Approximately 30% of specimens harboring non-B subtypes were underquantified by at least -0.51 log10 in Amplicor v1.5 versus 5% underquantified in G2 real-time PCR. Discrepant results were also obtained with subtype B samples (14% underquantified by Amplicor v1.5 vs. 7% by G2 real-time PCR). Similar percentages were observed when comparing results obtained with the G2 real-time PCR assay with those obtained using the Versant assay. Addressing HIV-1 diversity, continual monitoring of HIV-1 RNA assays, together with molecular epidemiology studies, is required to improve the accuracy of all HIV RNA assays.

  11. Strategy for the maximization of clinically relevant information from hepatitis C virus, RT-PCR quantification.

    LENUS (Irish Health Repository)

    Levis, J


    BACKGROUND: The increasing clinical application of viral load assays for monitoring viral infections has been an incentive for the development of standardized tests for the hepatitis C virus. OBJECTIVE: To develop a simple model for the prediction of baseline viral load in individuals infected with the hepatitis C virus. METHODOLOGY: Viral load quantification of each patient\\'s first sample was assessed by RT-PCR-ELISA using the Roche MONITOR assay in triplicate. Genotype of the infecting virus was identified by reverse line probe hybridization, using amplicons resulting from the qualitative HCV Roche AMPLICOR assay. RESULTS: Retrospective evaluation of first quantitative values suggested that 82.4% (n=168\\/204) of individuals had a viral load between 4.3 and 6.7 log(10) viral copies per ml. A few patients (3.4%; n=7\\/204) have a serum viremia less than the lower limit of the linear range of the RT-PCR assay. Subsequent, prospective evaluation of hepatitis C viral load of all new patients using a model based on the dynamic range of viral load in the retrospective group correctly predicted the dynamic range in 75.9% (n=33\\/54). CONCLUSION: The dynamic range of hepatitis C viremia extends beyond the linear range of the Roche MONITOR assay. Accurate determination of serum viremia is substantially improved by dilution of specimens prior to quantification.

  12. Global cost modeling analysis of HIV-1 and HCV viral load assays. (United States)

    Elbeik, Tarek; Chen, Yi-Ming Arthur; Soutchkov, Serguei V; Loftus, Richard A; Beringer, Scott


    This review addresses hidden costs associated with the Bayer VERSANT assay, Roche AMPLICOR MONITOR test and COBAS AMPLICOR MONITOR test and how these influence the final per reportable cost to a testing laboratory in resource-rich and -poor countries. An in-depth evaluation and recommendation of the most cost-effective approach for these tests is presented. The analyses demonstrate the need for manufacturers to consider labor and supply costs when marketing a kit in resource-poor countries, noting that marketing strategies need to change. In the absence of any proven monitoring alternative, emphasis is placed on increasing market share to promote significant reduction in kit prices to suit the demands of markets in resource-poor countries. Finally, recommendations are made to improve the overall cost structure of viral load testing. This review is intended as a tool to optimize assay usage in attaining the lowest performance costs by assay and is not to endorse any test, as will become apparent.

  13. Impact of occult HBV infection in HIV/HCV co-infected patients: HBV-DNA detection in liver specimens and in serum samples. (United States)

    Fabris, Paolo; Biasin, Maria R; Giordani, Maria T; Berardo, Laura; Menini, Vania; Carlotto, Antonio; Miotti, Maria G; Manfrin, Vinicio; Baldo, Vincenzo; Nebbia, Gaia; Infantolino, Domenico


    Prevalence and impact of occult HBV infection in HIV positive patients is controversial. The aims of this study were to determine the prevalence of occult HBV infection and its impact on histological and virological parameters. 52 HIV/HCV (but HBsAg-negative) co-infected patients, 29 HBsAg and anti-HCV negative chronic hepatitis, and 20 HBsAg positive chronic hepatitis controls were studied. DNA was extracted from frozen biopsies and amplified with primers for S, C and X regions, and for (ccc) HBV-DNA. Sera were tested for HBV-DNA with two quantitative assays (Cobas Amplicor HBV Monitor, and the real-time COBAS (r) Taqman HBV Test, Roche Diagnostics, UK). Occult HBV infection was detected in 7 (13.4%) liver biopsies of the study group, and in none case of the non viral chronic hepatitis group (p=0.04). All serum samples were HBV-DNA negative with Cobas Amplicor HBV monitor assay, while 3 cases were found positive with real time PCR. Statistical analysis didn't show any impact of occult HBV infection on liver histology, CD4+ cells count, HIV and HCV load, and ALT levels. Occult B infection is relatively frequent in HIV/HCV co-infected patients, and is underestimated by common HBV-DNA serological assays. However, it doesn't seem to exert a relevant impact.

  14. The Usefulness of Defining Rapid Virological Response by a Very Sensitive Assay (TMA) during Treatment of HCV Genotype 2/3 Infection

    DEFF Research Database (Denmark)

    Dalgard, Olav; Martinot-Peignoux, Michelle; Verbaan, Hans;


    The aim of this study was to determine in patients with HCV genotype 2 or 3 the performance at week 4 of two assays with different sensitivities for HCV RNA detection, for the prediction of SVR and stratification for treatment duration (14 and 24 weeks). Recruitment was from two trials comparing 14...... and 24 weeks treatment to patients with rapid virological response (RVR) (n = 550). RVR was originally defined as HCV RNA HCV-RNA was prospectively...... measured with COBAS Amplicor V2, Roche (CA) (lower detection limit 50 IU/ml) and retrospectively assessed with VERSANT HCV-RNA Qualitative Assay, Siemens (TMA) (lower limit detection 10 IU/ml). Genotype 3 was present in 80% and genotype 2 in 20%. A SVR was achieved in 82%. At week 4 HCV...

  15. Prevalence of Chlamydia infection among women visiting a gynaecology outpatient department: evaluation of an in-house PCR assay for detection of Chlamydia trachomatis

    Directory of Open Access Journals (Sweden)

    Patel Achchhe L


    Full Text Available Abstract Background Screening women for Chlamydia trachomatis infection in developing countries is highly desirable because of asymptomatic infection. The existing diagnostic methods in developing countries are not effective and their sensitivity fall below 45.0% which leads to further spread of infection. There is an urgent need for improved and cost effective diagnostic tests that will reduce the burden of sexually transmitted infections in the developing world. Methods Prevalence of C. trachomatis infection among women visiting gynaecology department of Hindu Rao hospital in Delhi, India was determined using Roche Amplicor Multi Well Plate kit (MWP as well as using in-house PCR assay. We used 593 endocervical swabs for clinical evaluation of the in-house developed assay against Direct Fluorescence Assay (DFA; Group I n = 274 and Roche Amplicor MWP kit (Group II, n = 319 samples and determined the sensitivity, specificity, positive predictive value (PPV, negative predictive value (NPV of the in-house developed assay. Results We detected 23.0% positive cases and there was a higher representation of women aged 18-33 in this group. An in-house PCR assay was developed and evaluated by targeting unique sequence within the gyrA gene of C. trachomatis. Specificity of the reaction was confirmed by using genomic DNA of human and other STI related microorganisms as template. Assay is highly sensitive and can detect as low as 10 fg of C. trachomatis DNA. The resolved sensitivity of in-house PCR was 94.5% compared with 88.0% of DFA assay. The high specificity (98.4% and sensitivity (97.1% of the in-house assay against Roche kit and availability of test results within 3 hours allowed for immediate treatment and reduced the risk of potential onward transmission. Conclusions The in-house PCR method is cost effective (~ 20.0% of Roche assay and hence could be a better alternative for routine diagnosis of genital infection by C. trachomatis to facilitate

  16. Tidal Interaction between a Fluid Star and a Kerr Black Hole Relativistic Roche-Riemann Model

    CERN Document Server

    Wiggins, P; Wiggins, Paul; Lai, Dong


    We present a semi-analytic study of the equilibrium models of close binary systems containing a fluid star (mass $m$ and radius $R_0$) and a Kerr black hole (mass $M$) in circular orbit. We consider the limit $M\\gg m$ where spacetime is described by the Kerr metric. The tidally deformed star is approximated by an ellipsoid, and satisfies the polytropic equation of state. The models also include fluid motion in the stellar interior, allowing binary models with nonsynchronized stellar spin (as expected for coalescing neutron star--black hole binaries) to be constructed. Tidal disruption occurs at orbital radius $r_{\\rm tide}\\sim R_0(M/m)^{1/3}$, but the dimensionless ratio of the black hole as well as on the equation of state and the internal rotation of the star. We find that the general relativistic tidal field disrupts the star at a larger $\\hat r_{\\rm tide}$ than the Newtonian tide; the difference is particularly prominent if the disruption occurs in the vicinity of the black hole's horizon. In general, $\\h...

  17. Cross-reactivity of amphetamine analogues with Roche Abuscreen radioimmunoassay reagents

    Energy Technology Data Exchange (ETDEWEB)

    Cody, J.T. (Air Force Drug Testing Laboratory, Brooks AFB, TX (USA))


    Cross-reactivity of amphetamine analogues with the Abuscreen amphetamine radioimmunoassay reagents was determined for both the standard and high specificity antibody systems. Compounds tested included 2-methoxyamphetamine, 4-hydroxymethamphetamine, 2,5-dimethoxyamphetamine (DMA), 4-bromo-2,5-dimethoxyamphetamine (DOB), 4-bromo-2,5-dimethoxy-beta-phenethylamine (BDMPEA), 3,4,5-trimethoxyamphetamine (TMA), 3,4-methylenedioxyamphetamine (MDA), N,N-dimethyl-3,4-methylenedioxyamphetamine and N-hydroxy-3,4-methylenedioxyamphetamine (N-OH MDA), 3,4-methylenedioxymethamphetamine (MDMA), 3,4-methylenedioxyethylamphetamine (MDEA), 2,5-dimethoxy-4-ethylamphetamine, 2,5-dimethoxy-4-methylamphetamine (DOM), and 3,4,5-trimethoxyphenethylamine (mescaline). Blank negative reference material was spiked with 1,000 to 100,000 ng/mL of the amphetamine analogue and used as sample in the assays. MDA was the only analogue that showed cross reactivity equal to or greater than that of amphetamine. None of the other analogue compounds demonstrated a positive result at even the highest concentration; however several showed depressed counts at various concentration levels.

  18. H08116: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-09-13 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  19. H08117: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1959-04-28 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  20. Defending the Freedom to Innovate: Faculty Intellectual Property Rights after "Stanford v. Roche" (United States)

    American Association of University Professors, 2014


    Tensions over faculty control of the fruits of their scholarship have been slowly building since the 1980s, but they have also intensified since late 2011. There have long been differences of opinion over ownership of patentable inventions, but over the last two years a number of universities have categorically asserted that they own these…

  1. H08115: NOS Hydrographic Survey , Roche Harbor and Mosquito Pass, Washington, 1954-06-29 (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The National Oceanic and Atmospheric Administration (NOAA) has the statutory mandate to collect hydrographic data in support of nautical chart compilation for safe...

  2. Clinical evaluation of the Roche Elecsys CMV IgG Avidity assay. (United States)

    Vauloup-Fellous, C; Lazzarotto, T; Revello, M G; Grangeot-Keros, L


    Congenital cytomegalovirus (CMV) infection has potentially severe consequences in newborns. The testing of pregnant women for CMV-specific antibodies may be useful for the identification of women at risk of transmitting the infection to the fetus. The determination of CMV IgG avidity helps to establish the timing of infection as IgG avidity matures during the course of infection. This study examines the performance of the Elecsys CMV IgG Avidity assay using preselected samples from patients at different phases of CMV infection. The Elecsys CMV IgG Avidity assay was tested at three sites using sequential samples from patients with recent primary CMV infection, as well as single samples from patients with recent primary or past CMV infection. The Elecsys assay discriminated well between early (low avidity) and late (high avidity) phases of infection in sequential serum samples. Overall, 98.8% of low-avidity samples corresponded to infection onset 90 days before sampling. The assay's sensitivity was 90-97%, with specificity ranging from 89 to 100%, depending on the consideration of gray-zone avidity values. Single samples from recent primary or past infection showed similar distributions of avidity results. The Elecsys CMV IgG Avidity assay results are in agreement with preselected samples from patients with primary or past CMV infection, showing that the test is an adequate predictor of the phase of infection.

  3. Roche genome sequencer FLX based high-throughput sequencing of ancient DNA

    DEFF Research Database (Denmark)

    Alquezar-Planas, David E; Fordyce, Sarah Louise


    Since the development of so-called "next generation" high-throughput sequencing in 2005, this technology has been applied to a variety of fields. Such applications include disease studies, evolutionary investigations, and ancient DNA. Each application requires a specialized protocol to ensure tha...

  4. Real-time PCR per HBV DNA: valutazione del nuovo sistema automatizzato COBAS AMPLIPREP™/COBAS TAQMAN™ HBV

    Directory of Open Access Journals (Sweden)

    Tiziano Allice


    Full Text Available Success of antiviral therapy for chronic hepatitis B is supported by highly sensitive PCR-based assays for Hepatitis B virus (HBV DNA. Nucleic acid extraction from biologic specimens is technically demanding and reliable PCR results depend it. Performances of the fully automatic system COBAS AmpliPrep™/COBAS TaqMan™ 48 (CAP/CTM (Roche, Branchburg, NJ for HBV DNA extraction and real -time PCR quantification were assessed and compared with the end-point PCR COBAS AMPLICOR HBV Monitor (CAHBM, Roche. Analytical evaluation with a proficiency panel showed that CAP/CTM quantitated HBV DNA levels in one single run over a wide dynamic range (7 logs with a close correlation between expected and observed values (r=0.976, interassay variability below 5%. Clinical evaluation as tested with samples from 92 HBsAg-positive patients, demonstrated excellent correlation with CAHBM (r=0.966, mean difference in quantitation: 0.36 log10 IU/ml. CAP/CTM detected 10% more viremic patients and longer period of residual viremia in those on therapy. In lamivudine (LAM-resistant patients, reduction of HBV DNA after 12 months of Adefovir (ADF was higher in the combination (LAM+ADF schedule than in ADF monotherapy (5.1 vs. 3.5 logs suggesting a benefit in continuing LAM. In conclusion,CAP/CTM can improve the management of HBV infection, the assessment of antiviral therapy and drug resistance, supporting further insights in the emerging area of drug resistance.

  5. Les roches basiques et ultrabasiques des Lacs Robert et le Trias de Chamrousse (Massif de Belledonne) Etude petrologique et geologique

    NARCIS (Netherlands)

    Tex, den E.


    La région que j’ai étudiée et relevée est située dans les Alpes françaises, à environ 4° longitude est et 50°15’ latitude nord, et à environ 2000 mètres d’altitude. Elle fait partie de la chaîne dite „chaîne de Belledonne” qui s’étend des massifs de Beaufort et du Grand Mont dans le N N E jusqu’à ce

  6. Analysis of heavy oils flows in saturated rocks; Analyse des ecoulements d'huiles lourdes dans les roches saturees

    Energy Technology Data Exchange (ETDEWEB)

    Luc Dormieux; Denis Garnier [Ecole Nationale des Ponts et Chaussees, 6 et 8 avenue Blaise Pascal Cite Descartes - Champs/Marne, 77455 Marne la Vallee Cedex 2, (France); Thierry Yalamas; Elisabeth Bemer; Jean-Francois Nauroy [Institut Francais du Petrole 1 et 4 avenue de Bois-Preau, 92852 Rueil-Malmaison Cedex, (France)


    In heavy oil reservoirs, the fluid is a viscous material, possibly nonlinear. In order to describe the flow of such oils in the rock, an extension of Darcy law to non Newtonian fluids is presented. Besides, oil production in this type of reservoir is responsible for rock erosion and the formation of a paste (slurry) made up of a mixture of oil and sand grains is observed. Based on a non linear homogenization technique, a modelling of the constitutive behavior of this slurry is proposed. (authors)

  7. About Jules et Jim: Some Figures of Matriarchy from Bachofen to Truffaut via Benjamin, Roché and Hessel

    Directory of Open Access Journals (Sweden)

    Michael Hollington


    Full Text Available Cet article essaie de relever quelques aspects d'un mouvement de pensée « matriarcale » du XXe siècle, parfois plutôt souterrain, et de montrer dans quelle mesure cette pensée fait partie du contexte du grand film des années soixante de François Truffaut. Il s'agit des hypothèses de Johann Jakob Bachofen (1815-1887, juriste et archéologue suisse, qui proposait la théorie d'un matriarcat primitif à l'origine de la société humaine. Dans un premier temps, en Méditerranée par exemple, existait selon lui un système communiste où l'idée de propriété privée était absente, fondé sur l'« hétaïrisme », où les rapports sexuels, organisés selon les vœux des femmes, étaient complètement libres.The purpose of this paper is, first, to provide a brief sketch map of a comparative nature of some linked, formative appropriations in Germany and elsewhere in the early part of this century of the Swiss jurist and archaeologist Johann Jakob Bachofen (1815-1887, famous for his influential but controversial theory of the precedence of primitive matriarchy over patriarchy in the Mediterranean and elsewhere.Este artίculo trata de señalar algunos aspectos de una corriente de pensamiento "matriarcal" del siglo veinte, a veces más bien subterránea, y muestra en qué medida dicho pensamiento forma parte del contexto de la famosa película de los años sesenta de François Truffaut. Se trata de las hipótesis de Johann Jakob Bachofen (1815-1887, jurista y arqueólogo suizo, quien propuso la teoría de un matriarcado primitivo en el origen de la sociedad humana. En un primer momento, en la región mediterránea, por ejemplo, dice que existía un sistema comunista carente de la idea de propiedad privada, y basado en el "hetaerismo", en el que las relaciones sexuales, organizadas según el voto de las mujeres, eran completamente libres.

  8. Analytical performance of the Roche LightCycler® Mycobacterium Detection Kit for the diagnosis of clinically important mycobacterial species.

    Directory of Open Access Journals (Sweden)

    Shaheed V Omar

    Full Text Available BACKGROUND: The LightCycler® Mycobacterium Detection Kit based on real-time PCR technology for the detection of Mycobacterium tuberculosis, Mycobacterium avium and Mycobacterium kansasii was recently developed. This study evaluated its analytical sensitivity, specificity and reproducibility. METHODOLOGY/PRINCIPAL FINDINGS: Plasmid standards were prepared and used to determine the limit of detection. The assay was also performed against organisms other than mycobacteria, other mycobacterial strains and interfering substances to exclude cross-reactivity and interference. Reference standards were prepared and tested to assess the assay's reproducibility. All PCR assays were performed using the LightCycler® 2.0 Instrument. The detection limit for M. tuberculosis was 28 copies per microlitre. Neither cross-reactivity nor interference occurred with non-mycobacterial organisms and substances tested. Overall reproducibility for consecutive measurements, run-to-run, lot-to-lot, day-to-day and laboratory-to-laboratory achieved a coefficient of variance of less than two percent. SIGNIFICANCE: The LightCycler® Mycobacterium Detection kit has shown to be a robust and accurate assay with the potential to be used as a rapid TB diagnostic test.

  9. Near-complete genome sequencing of swine vesicular disease virus using the Roche GS FLX sequencing platform

    DEFF Research Database (Denmark)

    Nielsen, Sandra Cathrine Abel; Bruhn, Christian Anders Wathne; Samaniego Castruita, Jose Alfredo;


    that is suitable for sequencing the complete protein-encoding sequences of SVDV isolates in which the RNA is relatively intact. The approach couples a single PCR amplification reaction, using only a single PCR primer set to amplify the near-complete SVDV genome, with deep-sequencing using a small fraction...

  10. Persistence of hepatitis C virus in a white population: associations with human leukocyte antigen class 1.

    LENUS (Irish Health Repository)

    Fanning, Liam J


    The aim of this study was to define novel associations between human leukocyte antigen (HLA) class 1 alleles and persistence or clearance of the hepatitis C virus (HCV) in a white population. All individuals in the study were seropositive for anti-HCV antibodies. Viral status was determined by the Roche HCV Amplicor test. HLA-A, -B, -C allelic group profile was molecularly defined by reverse line probe hybridization. The strongest individual allelic group associations with persistent HCV infection were HLA A*11 (p = 0.044) and Cw*04 (p = 0.006). However, only the HLA C*04 association survived correction for multiple comparisons. Further analysis of alleles in linkage with HLA Cw*04 revealed that the haplotype HLA A*11, Cw*04 was present in 11 individuals, 10 of whom were viremic (p = 0.05). No gene dosage effect was observed. No association between HLA class 1 allelic groups and aviremia and virus load was evident in this white population. HLA B*44 is associated with low virus load in human immunodeficiency virus disease, but this association was not evident in this HCV-infected population. Novel HLA class 1 alleles associated with persistence of HCV have been identified.

  11. Single genome amplification of proviral HIV-1 DNA from dried blood spot specimens collected during early infant screening programs in Lusaka, Zambia. (United States)

    Seu, Lillian; Mwape, Innocent; Guffey, M Bradford


    The ability to evaluate individual HIV-1 virions from the quasispecies of vertically infected infants was evaluated in a field setting at the Centre for Infectious Disease Research in Zambia. Infant heel-prick blood specimens were spotted onto dried blood spot (DBS) filter paper cards at government health clinics. Nucleic acid was extracted and used as a template for HIV-1 proviral DNA detection by a commercial Amplicor HIV-1 PCR test (Roche, version 1.5). On samples that tested positive by commercial diagnostic assay, amplification of DNA was performed using an in-house assay of the 5' and 3' region of the HIV-1 genome. Additionally, fragments covering 1200 nucleotides within pol (full length protease and partial reverse transcriptase) and 1400 nucleotides within env (variable 1-variable 5 region) were further analyzed by single genome amplification (SGA). In summary, we have demonstrated an in-house assay for amplifying the 5' and 3' proviral HIV-1 DNA as well as pol and env proviral DNA fragments from DBS cards collected and analyzed entirely in Zambia. In conclusion, this study shows the feasibility of utilizing DBS cards to amplify the whole proviral HIV-1 genome as well as perform SGA on key HIV-1 genes.

  12. Diagnosis of Neisseria gonorrhoeae Using Molecular Beacon

    Directory of Open Access Journals (Sweden)

    Divya Sachdev


    Full Text Available Neisseria gonorrhoeae is an important sexually transmitted diseases (STD causing pathogen worldwide. Due to absence of an affordable diagnostic assay, routine screening of gonococcal infection becomes impossible in developing countries where infection rates are maximum. Treatment is given on the basis of symptoms alone which leads to spread of infection. Thus, development of a rapid, sensitive, specific, and PCR based visual diagnostic assay suitable for developing countries, required for better disease management, is aimed at in present study. Endocervical swabs were collected from patients visiting gynecology department of various hospitals in Delhi. In-house PCR based assay was developed and modified to visual assay using molecular beacon for end-point detection. It was evaluated against Roche AMPLICOR NG kit and rmp gene. Specificity of beacon was confirmed by competition experiments. Diagnostic test was 98.21% specific and 99.59% sensitive whereas negative and positive predicted value were 99.40% and 98.78%, respectively. We also observed that twice the concentration (2X of premix was stable at 4°C for 4 months and dry swab samples gave concordant results with that of wet swabs. These features make the test best suitable for routine diagnosis of genital infections in developing countries.

  13. Stable hepatitis C virus RNA detection by RT-PCR during four days storage

    Directory of Open Access Journals (Sweden)

    Horsmans Yves


    Full Text Available Abstract Background Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4°C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples. Methods Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 – 25.4°C or at 4°C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems. Results The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4°C. Conclusions We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4°C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.

  14. Lyophilized standards for the calibration of real time PCR assay for hepatitis C virus RNA

    Institute of Scientific and Technical Information of China (English)

    WANG Lu-nan; WU Jian-min; DENG Wei; SHEN Zi-yu; CHEN Wen-xiang; LI Jin-ming


    Background Since October 1997, an international standard for hepatitis C virus (HCV) nucleic acid amplification technology assay, 96/790, has been available. We compared a series of lyophilized standards with known HCV RNA concentrations against the international standard in fluorescence quantitative PCR detection.Methods A series of lyophilized sera were calibrated by ROCHE COBAS AMPLICOR HCV Monitor test against the international standard and sent to various manufacturers to analyse the samples using their own kits.Then calibration curves from the series were compared with that obtained from the external standard calibration curve with the manufacture's series.Results The standard calibration curve with the series of lyophilized serum showed an excellent correlation(R2>0.98), slope and intercept that were similar to those from the manufacture's series. When the standard calibration curve from the series of lyophilized standards were used to define the values of the given sample,lower coefficients of variation between kits from different manufactures were obtained.Conclusion The results showed that the lyophilized standards could be used to setup the standard calibration curve for clinical HCV RNA quantitative PCR detection.

  15. Single-step PCR in molecular diagnosis of hepatitis C virus infection. (United States)

    Farma, E; Boeri, E; Bettini, P; Repetto, C M; McDermott, J; Lillo, F B; Varnier, O E


    The diagnostic utility of two PCR systems and three PCR detection methods for hepatitis C virus (HCV) RNA was evaluated in serum samples. A nested PCR was considered the reference assay and was compared with two single-step PCR methods: the first is based on the detection of PCR products by liquid hybridization with a 32P-end-labeled probe, and the second is the Roche Amplicor colorimetric assay using microwell plate hybridization with a specific nucleic acid probe. Using the Pelicheck HCV RNA Eurohep genotype 1 proficiency panel, our laboratory achieved medium-high levels of performance with all three methods. The highest sensitivity was, however, observed with the isotopic single-step PCR (ss-PCR) method. The analytical sensitivity of ss-PCR with isotopic detection and ss-PCR with colorimetric detection was identical to that of nested PCR, with a 100% result concordance. Comparison of ss-PCR with enzyme-linked immunosorbent and RIBA assays in the analysis of clinical samples showed a high concordance. ss-PCR methods appear more suitable for diagnostic application. Nevertheless, HCV RNA PCR cannot be considered a screening assay; it should be requested in the presence of reactive serology or specific clinical symptomatology with altered liver parameters, and it is a potential tool for the follow-up of patients with HCV infection.

  16. Sieroprevalenza di infezione da HBV e HCV tra pazienti in dialisi

    Directory of Open Access Journals (Sweden)

    Rosa Anna Leone


    Full Text Available The aim of the present study was to investigate the seroprevalence of HBV and HCV among dialysis patients in the Lamezia Terme (CZ area during the period 1999-2002. Sera from 63 patients in haemodialysis (HD and 10 patients in peritoneal dialysis (PD were analyzed with a follow-up every three months for HBsAg, HBcAb, HBsAb, anti-HCV and anti-HIV (Elisa Test,AxSYM,Abbott;we analyzed reactive sera for anti-HCV by using supplemental test (RIBA Test, Ortho; we also looked for viremia (RT-PCR Amplicor, Roche Diagnostics and HCV genotypes (Inno-Lipa HCV II, Innogenetics.The results show that, among the HD patients, 3 were HBsAg positive (Chronic Infection and 7 HBcAb and HBsAb positive/HBsAg negative (Passed Infection; 14 individuals were anti-HCV positive. No patients in PD were positive for HBV and HCV markers.The prevalence of chronic HBV infection was 4.8% (instead of 3% in other Dialysis Units, that of anti-HCV positive was 22% (in others 24%- 33%; among anti-HCV positive patients, the HCV-RNA prevalence was 79% (instead of 80%; the most recurrent HCV genotype was 2a/2c (instead of 1b in general population.These findings lead us to hypothesize that the environmental transmission in the dialysis setting is tightly correlated to the risk of HBV and HCV infection.

  17. Validation of the Gen-Probe Aptima qualitative HIV-1 RNA assay for diagnosis of human immunodeficiency virus infection in infants. (United States)

    Fiscus, Susan A; McMillion, Takesha; Nelson, Julie A E; Miller, William C


    The qualitative Roche HIV-1 DNA Amplicor assay has been used for the past 20 years to diagnose HIV infection in infants and young children but is being phased out; hence, alternative assays must be found. The Gen-Probe Aptima qualitative HIV-1 RNA assay is currently the only FDA-cleared HIV-1 nucleic acid assay approved for diagnosis, but data on the use of this assay with infant plasma are limited. We assessed Aptima's performance using control material for reproducibility and limit of detection and 394 plasma samples (0.2 to 0.5 ml) from HIV-exposed infected and uninfected infants and children for analytical sensitivity and specificity. Assays to assess within-run repeatability and between-run reproducibility indicated that the controls with 10,000 (5 of 5), 200 (5 of 5), 100 (16 of 16), 50 (12 of 12), and 25 (20 of 20) HIV-1 RNA copies/ml (cp/ml) were always positive, and negatives were always negative (20 of 20). The limit of detection was 14 cp/ml, as determined by probit analysis. The analytic sensitivity of the assay was 99.5% (189/190 samples; 95% confidence interval [CI], 97.1 to 99.9%) and specificity was 99.5% (199/200 samples; 95% CI, 97.2 to 99.9%). These results suggest that the assay is suitable for early infant diagnosis of HIV-1.

  18. Evaluation of five kits for screening Chlamydia trachomatis%五种不同沙眼衣原体实验室筛查试剂检测性能评价

    Institute of Scientific and Technical Information of China (English)

    赵广录; 张娟娟; 王峰; 于微; 洪福昌; 蓝丽娜; 吴肖冰; 张春来; 冯铁建


    Objective To evaluate five kits for screening Chlamydia trachomatis (CT) by comparing the CT screening test results with Roche Cobas Amplicor test . Methods A proportionate stratified sampling was taken a-mong 32 medical institutions, and 1986 urogenital specimens were collected from STD , gynecology and urology clinics from September to November 2009. Various Chlamydia trachomatis detection methods were evaluated by comparing with Roche Cobas Amplicor test as a "golden standard", Results By comparing with Roche Cobas Amplicor test, the sensitivity of three kinds of gold-Immunochromatographic assay (ICA) , I. E. Rapid-test kits of ICA1, ICA2 and ICA3 was 34. 48%, 15. 22% and 52.53% respectively, The specificity was 99.45%, 98.04% and 98. 93% respectively, the positive predictive value (PPV) was 93. 75% ,63. 64% and 92. 86% , the negative predictive value (NPV) was 86. 36%, 83. 68% and 88. 73%. The sensitivity of the two polymerase chain reaction (PCR) kits of PCR1 and PCR2 was 70. 09% and 96. 43% respectively ; their specificity was 99. 44% and 100. 00% respectively, the positive predictive value (PPV) was 96. 15% and 100.00% , and the negative predictive value (NPV) was 94. 34% and 99. 31%. Four types of samples tested by ICA kits showed obvious differences in sensitivity and specificity. But no similar results were found in PCR detection test. Conclusions The performances of immuno-chromatographic test kits produced by these three companies are significantly different. But the overall level is lower than that of the PCR detection kits. In addition, the type of specimen also affects the result of Chlamydia trachomatis detection. The immunochromatography test kit is more suitable for female vaginal and cervical samples.%目的 通过对不同商品化沙眼衣原体(CT)检测试剂的检测结果,与Roche Cobas Amplicor检测结果的分析比较,评价5种沙眼衣原体检测试剂的性能.方法 采用分层按比例的方法抽取32家医

  19. Does chronic alcohol use by HIV-infected patients on d4T/3TC/NVP drug regimen effect the HIV viral load and what is the therapeutic window of the drugs, CD4+ count and WBC count in patients with high viral load during the 9 months period of follow up?

    Directory of Open Access Journals (Sweden)

    Godfrey S. Bbosa


    Full Text Available The study investigated the effects of chronic alcohol use on HIV viral load in HIV-infected patients on d4T/3TC/NVP drug regimen during 9 months follow up period. It also determined plasma drug concentrations of d4T, 3TC and NVP; CD4+ and WBC counts for patients with high HIV viral load. A case-control study using repeated measures with serial measurements was used. A total of 41 patients (20 alcohol group and 21 control group were screened for alcohol use using WHO AUDIT tool and chronic alcohol use biomarkers. Blood sampling was done at 3 month intervals for a period of 9 months. HIV viral load was determined using Roche Amplicor HIV-1 monitor test, version 1.5 (Amplicor. The d4T, 3TC and NVP concentrations were determined by Shimadzu Class-VPTM HPLC Chromatography data system version 6.1. The CD4+ cell count was determined using FACSCalibur flow cytometer. The WBC was determined using automated hematological Coulter CBC-5 Hematology Analyzer system. Results show that % patients with HIV viral load ≥400 copies/ml in control group was highest (23.8%, n=5 at 3 month while in chronic alcohol use group, it was at 0 month (35%, n=7 for both WHO AUDIT tool and chronic alcohol-use biomarkers groups. Generally patients with high viral load ≥400 copies/ml was observed in chronic alcohol use as compared to control group in both WHO AUDIT tool and biomarkers group despite of patients having high steady state d4T, 3TC and NVP plasma drug concentrations in circulation that is available to suppress HIV virus. The high viral load could be associated with the emergence of resistance of the HIV virus and these patients generally had a low CD4+ cell count. Some of these patients had no detectable d4T plasma drug concentrations in circulation and most of them with high viral load had sub-therapeutic NVP plasma drug concentrations in their blood circulation. Chronic ethanol use by HIV-infected patients on d4T/3TC/NVP drug regimen increased HIV viral load and

  20. Treatment seeking behaviors related to gonorrhea among female sex workers in 7 cities in Indonesia

    Directory of Open Access Journals (Sweden)

    Roselinda Roselinda


    Full Text Available Abstrak Latar belakang:Gonore merupakan salah satu infeksi menular seksual yang menjadi permasalahan besar kesehatan terutama pada wanita penjaja seks (WPS di Indonesia. Tujuan dari artikel ini adalah untuk melihat hubungan antara pola pencarian pengobatan gonore. Metode:Data berasal dari studi potong lintang dengan responden WPS yang dipilih secara cluster random sampling dari 7 kota (Timika, Yogyakarta, Kupang, Samarinda, Pontianak, Makassar dan Tangerang di Indonesia pada tahun 2007. Diagnosis gonore berdasarkan hasil pemeriksaan Polymerase Chain Reaction (PCR menggunakan Amplicor CT/NG dari Roche yang telah disetujui oleh World Health Orgazation (WHO sebagai alat skrining gonore. Hasil:Proporsi responden yang menderita gonore sebesar 26.1% (404/1750. Persentase penderita gonore yang melakukan upaya pengobatan terdistribusi hampir sama dengan yang mengunjungi fasilitas kesehatan / dokter dengan yang membeli obat sendiri. Subyek yeng melakukan pengobatan tradisional memiliki risiko 44% lebih tinggi menderita gonore dibandingkan dengan subyek yang melakukan pengobatan di fasilitas kesehatan / dokter [risiko relatif suaian (RRa = 1,44; P = 0.044]. Sedangkan subyek yang tidak diobati dibandingkan dengan yang berobat ke fasilitas kesehatan / dokter lebih berisiko 55% menderita gonore (RRa = 1.55; P = 0.002.Kesimpulan: Wanita penjaja seks yang melakukan maupun yang tidak pengobatan tradisional dibandingkan dengan yang mengunjungi fasilitas kesehatan/dokter memiliki risiko yang lebih tinggi menderita gonore. (Health Science Indones 2013;2:87-92Kata kunci:gonore, wanita penjaja seks, IndonesiaAbstractBackground:Gonorrhea is one of sexually transmitted infections that have become a major health problem especially among female sex workers (FSW in Indonesia. The objective of this article is to identify the relationship between treatment seeking behaviors, the sites of study and gonorrhea among FSW. Methods: The data that analyzed derived from cross

  1. Variables that influence HIV-1 cerebrospinal fluid viral load in cryptococcal meningitis: a linear regression analysis

    Directory of Open Access Journals (Sweden)

    Cecchini Diego M


    Full Text Available Abstract Background The central nervous system is considered a sanctuary site for HIV-1 replication. Variables associated with HIV cerebrospinal fluid (CSF viral load in the context of opportunistic CNS infections are poorly understood. Our objective was to evaluate the relation between: (1 CSF HIV-1 viral load and CSF cytological and biochemical characteristics (leukocyte count, protein concentration, cryptococcal antigen titer; (2 CSF HIV-1 viral load and HIV-1 plasma viral load; and (3 CSF leukocyte count and the peripheral blood CD4+ T lymphocyte count. Methods Our approach was to use a prospective collection and analysis of pre-treatment, paired CSF and plasma samples from antiretroviral-naive HIV-positive patients with cryptococcal meningitis and assisted at the Francisco J Muñiz Hospital, Buenos Aires, Argentina (period: 2004 to 2006. We measured HIV CSF and plasma levels by polymerase chain reaction using the Cobas Amplicor HIV-1 Monitor Test version 1.5 (Roche. Data were processed with Statistix 7.0 software (linear regression analysis. Results Samples from 34 patients were analyzed. CSF leukocyte count showed statistically significant correlation with CSF HIV-1 viral load (r = 0.4, 95% CI = 0.13-0.63, p = 0.01. No correlation was found with the plasma viral load, CSF protein concentration and cryptococcal antigen titer. A positive correlation was found between peripheral blood CD4+ T lymphocyte count and the CSF leukocyte count (r = 0.44, 95% CI = 0.125-0.674, p = 0.0123. Conclusion Our study suggests that CSF leukocyte count influences CSF HIV-1 viral load in patients with meningitis caused by Cryptococcus neoformans.

  2. Risk factors for active trachoma and ocular Chlamydia trachomatis infection in treatment-naive trachoma-hyperendemic communities of the Bijagos Archipelago, Guinea Bissau.

    Directory of Open Access Journals (Sweden)

    Anna R Last


    Full Text Available Trachoma, caused by ocular infection with Chlamydia trachomatis, is hyperendemic on the Bijagós Archipelago of Guinea Bissau. An understanding of the risk factors associated with active trachoma and infection on these remote and isolated islands, which are atypical of trachoma-endemic environments described elsewhere, is crucial to the implementation of trachoma elimination strategies.A cross-sectional population-based trachoma prevalence survey was conducted on four islands. We conducted a questionnaire-based risk factor survey, examined participants for trachoma using the World Health Organization (WHO simplified grading system and collected conjunctival swab samples for 1507 participants from 293 randomly selected households. DNA extracted from conjunctival swabs was tested using the Roche Amplicor CT/NG PCR assay. The prevalence of active (follicular and/or inflammatory trachoma was 11% (167/1508 overall and 22% (136/618 in 1-9 year olds. The prevalence of C. trachomatis infection was 18% overall and 25% in 1-9 year olds. There were strong independent associations of active trachoma with ocular and nasal discharge, C. trachomatis infection, young age, male gender and type of household water source. C. trachomatis infection was independently associated with young age, ocular discharge, type of household water source and the presence of flies around a latrine.In this remote island environment, household-level risk factors relating to fly populations, hygiene behaviours and water usage are likely to be important in the transmission of ocular C. trachomatis infection and the prevalence of active trachoma. This may be important in the implementation of environmental measures in trachoma control.

  3. Chlamydia trachomatis infection and sexual behaviour among female students attending higher education in the Republic of Ireland.

    LENUS (Irish Health Repository)

    O'Connell, Emer


    BACKGROUND: There are no prevalence data on Chlamydia trachomatis relating to female students attending higher education available for the Republic of Ireland. This information is required to guide on the necessity for Chlamydia screening programmes in higher education settings. This research aimed to determine the prevalence of and predictive risk factors for Chlamydia trachomatis genital infection among female higher education students in Ireland. METHODS: All females presenting during one-day periods at Student Health Units in three higher education institutions in two cities in the Republic of Ireland were invited to participate. Participants completed a questionnaire on lifestyle and socio-demographic factors and provided a urine sample. Samples were tested for C. trachomatis DNA by a PCR based technique (Cobas Amplicor, Roche). To examine possible associations between a positive test and demographic and lifestyle risk factors, a univariate analysis was performed. All associations with a p value < 0.05 were included in a multivariate logistic regression analysis. RESULTS: Of the 460 sexually active participants 22 tested positive (prevalence 4.8%; 95% CI 3.0 to 7.1%). Variables associated with significantly increased risk were current suggestive symptoms, two or more one-night stands and three or more lifetime sexual partners. The students displayed high-risk sexual behaviour. CONCLUSION: The prevalence of C. trachomatis infection and the lack of awareness of the significance of suggestive symptoms among sexually experienced female students demonstrate the need for a programme to test asymptomatic or non-presenting higher education students. The risk factors identified by multivariate analysis may be useful in identifying those who are most likely to benefit from screening. Alcohol abuse, condom use, sexual behaviour (at home and abroad) and, knowledge of sexually transmitted infections (STIs) (including asymptomatic nature or relevant symptoms) were

  4. Stratigraphic and technological evidence from the middle palaeolithic-Châtelperronian-Aurignacian record at the Bordes-Fitte rockshelter (Roches d’Abilly site, Central France)

    DEFF Research Database (Denmark)

    Aubry, Thierry; Dimuccio, Luca Antonio; Almeida, Miguel;


    This paper presents a geoarchaeological study of Middle and Upper Palaeolithic (Châtelperronian, Aurignacian and Solutrean) occupations preserved at the Bordes-Fitte rockshelter in Central France. The lithostratigraphic sequence is composed of near-surface sedimentary facies with vertical and lat...

  5. Influence aqueous solutions on the mechanical behavior of argillaceous rocks; Influence des solutions aqueuses sur le comportement mecanique des roches argileuses

    Energy Technology Data Exchange (ETDEWEB)

    Wakim, J


    The hydration of the shale with an aqueous solution induces a swelling deformation which plays an important role in the behaviour of the structures excavated in this type of grounds. This deformation is marked by a three-dimensional and anisotropic character and involves several mechanisms like adsorption, osmosis or capillarity. Several researches were dedicated to swelling and were often much debated due to the complexity of the implied phenomena. The goal of this thesis is therefore to contribute to a better understanding of shale swelling when the rock is confined and hydrated with an aqueous solution. The main part of the work accomplished was related to the Lorraine shale and to the Tournemire shale. To characterize swelling and to identify the main governing parameters, it was necessary to start the issue with an experimental approach. Many apparatus were then developed to carry out tests under various conditions of swelling. In order to facilitate the interpretation of the tests and thereafter the modelling of the behaviour, the experimental procedure adopted consisted of studying first the mechanical aspect and then the chemical aspect of swelling. In the mechanical part, swelling was studied by imposing on the sample a mechanical loading while maintaining during the tests the same aqueous solution. The principal parameters which were studied are the effect of the lateral conditions on axial swelling (impeded strain or constant stress) as well as the influence of the axial stress on radial swelling. The anisotropy of swelling was studied by carrying out, for different orientations of the sample, tests of free swelling, impeded swelling and uniaxial swelling. These various mechanical tests allowed to study the three-dimensional anisotropic swelling in all the conditions and to select the most appropriate test to be used in the second phase of the research. The precise analysis performed to explain the mechanisms behind the swelling of an argillaceous rock when it is in contact of water showed that the mechanical response is ruled mainly by electrochemical phenomena which operate between the solution elements and the clay layers. This important result oriented the research to develop better the role of the chemistry in the behaviour. Swelling was therefore analysed by imposing on the sample a chemical loading while maintaining constant the axial stress. Monovalent and divalent salts which were used with various concentrations are the sodium chloride (NaCl), the potassium chloride (KCl) and the calcium chloride (CaCl{sub 2}). The foreseen objective was to analyze the effect of the chemical path with decreasing or cyclic concentrations and to highlight the role of the solution normality, the type of salt and the anisotropy on the axial swelling. The experimental results obtained allowed the development of a new rheological model which takes into account the time, the anisotropy, the applied loading and the chemistry of the solution. This model was implemented in a finite element code to simulate simple cases and to prove its validity. (author)

  6. Fractures inside crystalline rocks. Effects of deformations on fluid circulations; Fractures dans les roches cristallines. Effets des deformations sur les circulations de fluides

    Energy Technology Data Exchange (ETDEWEB)

    Gentier, S


    The modeling of fluid flows inside granite massifs is an important task for the evaluation of the feasibility of radioactive waste storage inside such formations. This document makes a synthesis of the works carried out since about 15 years, in particular by the French bureau of geological and mining research (BRGM), about the hydro-mechanical behaviour of a fracture and about the hydrodynamical characterization of fracture networks inside crystalline rocks: 1 - introduction; 2 - hydro-mechanical behaviour under normal stress: experimental results (hydro-mechanical behaviour, flow regimes, mechanical behaviour, test protocol, complementary tests, influence of samples size), geometrical interpretation of experimental results (relation with walls geometry, relation with voids geometry, relation with contacts geometry), hydro-mechanical modeling (hydraulic modeling, mechanical modeling); 3 - from the hydro-mechanical behaviour under normal stress to the coupling with heat transfers and chemistry: experiment for the study of the chemo-thermo-hydro-mechanical coupling (experimental results, relation with walls morphology), thermo-hydro-mechanical experiments, thermo-hydro-chemical experiments with fractures, conclusions; 4 - hydro-mechanical behaviour during shear: experimental results, geometrical interpretation (relation with the geometry of damaged zones, relation with voids geometry, relation with walls geometry), hydro-mechanical modeling (mechanical modeling, hydro-mechanical modeling of the behaviour during shear). (J.S.)

  7. Cicatrization of the damaged zone around a tunnel in low permeable rocks; Cicatrisation de la zone endommagee autour du tunnel dans des roches peu permeables

    Energy Technology Data Exchange (ETDEWEB)

    Hamza, R.O


    Three types of clays have been studied in this work: the Mol clay (plastic clay), the Mont Terri clay (opalinus clay) and the East clay (stiff clay). This work is divided into three parts: an experimental part which has allowed to identify the cicatrization phenomenon and the cracks closing. Through several tests has been determined the evolution of the permeability of the clayey samples which are undamaged or damaged with time and according to the different applied hydro-mechanical loadings. In order to describe the cicatrization phenomenon, a rheological law has been developed from the experimental observations and under some hypotheses. The parameters of the cicatrization model have been determined by numerical simulation. In order to take into account the structures effects at a large scale, the modelling of a tunnel has allowed to better identify the cicatrization phenomenon and to analyze the long term stability of this work. (O.M.)

  8. Creep and damage in argillaceous rocks: microstructural change and phenomenological modeling; Fluage et endommagement des roches argileuses: evolution de la microstructure et modelisation phenomenologique

    Energy Technology Data Exchange (ETDEWEB)

    Fabre, G


    The underground radioactive waste disposal far exceeds the period of exploitation of common civil engineering works. These specific projects require to predict the irreversible deformations over a large time scale (several centuries) in order to assess the extension and to forecast the evolution of the EDZ (Excavation Damage Zone) around the cavity. In this study, the viscosity of three sedimentary argillaceous rocks has been studied under different conditions of uniaxial compression: static or cyclic creep tests, monotonic and quasistatic tests, performed across various strata orientations. Argillaceous rocks are studied as a possible host layer for radioactive waste disposals. Indeed, they present some of the physical characteristics and mechanical properties, which are essential for being a natural barrier: low permeability, high creep potential and important holding capacity of radioactive elements. The purpose of the experimental study was to shed some light over the mechanisms governing the development of delayed deformations and damage of argillaceous rocks. It relates three rocks: an argillite from East of France, a Tournemire argillite and a marl from Jurassic Mountains. On atomic scale, viscoplastic deformations are due to irreversible displacements of crystalline defects, called dislocations. The experimental study was also supplemented with observations on thin sections extracted from the argillite and marl samples using a SEM. The aim was to identify the mechanisms responsible for the time-dependent behaviour on a microstructural scale. Analytical simulations of the mechanical behaviour of the three rocks gave parameters used in different viscoplastic models. The best modeling was obtained with the viscoplastic model which takes account of the development of volumetric strains and of the damage anisotropy. (author)

  9. Dynamics of stellar wind in a Roche potential: implications for (i) outflows & periodicities relevant to astronomical masers, and (ii) generation of baroclinicity

    CERN Document Server

    Singh, Nishant K


    We study the dynamics of stellar wind from one of the bodies in the binary system, where the other body interacts only gravitationally. We focus on following three issues: (i) we explore the origin of observed periodic variations in maser intensity; (ii) we address the nature of bipolar molecular outflows; and (iii) we show generation of baroclinicity in the same model setup. From direct numerical simulations and further numerical modelling, we find that the maser intensity along a given line of sight varies periodically due to periodic modulation of material density. This modulation period is of the order of the binary period. Another feature of this model is that the velocity structure of the flow remains unchanged with time in late stages of wind evolution. Therefore the location of the masing spot along the chosen sightline stays at the same spatial location, thus naturally explaining the observational fact. This also gives an appearance of bipolar nature in the standard position-velocity diagram, as has ...

  10. Reduction of Polymer Adsorption on Reservoir Rocks Réduction de l'adsorption des polymères sur les roches réservoirs

    Directory of Open Access Journals (Sweden)

    Chauveteau G.


    Full Text Available The adsorption properties of polyacrylamides and xanthans on mineral surfaces carrying silanol and aluminol groups such as sand and kaolinite are described. The influence of the main parameters such as the nature of adsorption sites, surface charge, chemical structure and conformation of polymer and interactions of mono- and divalent ions with polymer and mineral surface has been investigated and interpreted. Some operating parameters in polymer flooding such as pH and salinity of injected solution, the nature of the polymer and its degree of ionicity were found to be determining factors from the adsorption level. The results give key elements for reducing adsorption by a proper choice of polymer nature and ionicity and of injection conditions. Les propriétés d'adsorption des polyacrylamides et des xanthanes sur des surfaces minérales portant des groupements silanols et aluminols comme le sable et la kaolinite ont été examinées. L'influence de différents paramètres est analysée : nature des sites d'adsorption, charge de surface, structure chimique et conformation du polymère, interactions des ions mono et divalents avec la surface. Il apparaît que le pH et la salinité de la solution injectée, la nature du polymère et, en particulier son degré d'ionicité, qui sont les paramètres opérationnels lors d'une injection de polymère destinée à augmenter le taux de récupération du pétrole, sont déterminants en ce qui concerne les niveaux d'adsorption. On en déduit les principaux moyens pour réduire l'adsorption dans un cas d'application donné.

  11. Approach to underground characterization of a disposal vault in granite; Methode de caracterisation souterraine d`une enceinte de stockage dans la roche granitique

    Energy Technology Data Exchange (ETDEWEB)

    Everitt, R.A.; Martin, C.D.; Thompson, P.M.


    The concept of disposing of nuclear fuel waste by sealing it in a disposal vault in the Canadian Shield is being investigated as part of the Canadian Nuclear Fuel Waste Management Program. Engineered and natural barriers would isolate the waste from the biosphere. Underground characterization and testing have been under way since 1983 at the Underground Research Laboratory in support of this program. This report draws on experience gained at the URL to recommend an approach to underground characterization to obtain information to optimize the design of the excavation and the engineered barriers, and to provide a baseline against which to monitor the performance of the facility during and following its operation.

  12. Monkeypox detection in rodents using real-time 3’minor groove binder Taqman assays on the Roche LightCycler, Laboratory Investigation 84:1200 - 1208 (United States)


    DNA 1000 MPV Zaire 79-I-05 10 + + MPXR Zaire 79-I-05 ( Cidofovir - resistant) 10 + + MPV Utrecht 10 + + MPV Sierra Leone 10 + + MPV 12003k 10 + + MPV...100 CMLR— Cidofovir -resistant Camelpox Somalia 100 CPX—cowpox Brighton Red (BR) 100 CPXR— Cidofovir -resistant cowpox Brighton Red 100 VAC...Vaccinia Copenhagen 100 VACR— Cidofovir -resistant Vaccinia Copenhagen 100 HSV-1—Herpes Simplex virus type 1 100 HSV-2—Herpes Simplex virus

  13. Prevalence of human papillomavirus in 5,072 consecutive cervical SurePath samples evaluated with the Roche cobas HPV real-time PCR assay

    DEFF Research Database (Denmark)

    Preisler, Sarah; Rebolj, Matejka; Untermann, Anette;


    New commercially available Human Papillomavirus (HPV) assays need to be evaluated in a variety of cervical screening settings. Cobas HPV Test (cobas) is a real-time PCR-based assay allowing for separate detection of HPV genotypes 16 and 18 and a bulk of 12 other high-risk genotypes. The aim...... of the present study, Horizon, was to assess the prevalence of high-risk HPV infections in an area with a high background risk of cervical cancer, where women aged 23-65 years are targeted for cervical screening. We collected 6,258 consecutive cervical samples from the largest cervical screening laboratory...... in Denmark serving the whole of Copenhagen. All samples were stored in SurePath media. In total, 5,072 samples were tested with cobas, Hybrid Capture 2 High Risk HPV DNA test (HC2) and liquid-based cytology. Of these, 27% tested positive on cobas. This proportion decreased by age, being 43% in women aged 23...

  14. Characterization of Rock Wettability Though Dielectric Measurements Caractérisation de la mouillabilité des roches au moyen de mesures diélectriques

    Directory of Open Access Journals (Sweden)

    Bona N.


    Full Text Available The wettability of glass filters and Berea sandstone was investigated using the electric response in the interval 10² - 10 (to the power of 8 Hz. The natural wettability of the materials was modified to get two different sets of samples, one with strong water and the other with strong oil wettability. The samples were saturated to various degrees up to 40% with deionized water or brine. Measurements showed that the electric responses of water-wet and oil-wet samples were markedly different and more complex than those predicted by two standard models. The dispersivity and the loss tangent were found to be the most suitable parameters to check the wettability of the samples. La mouillabilité de filtres de verre et de grès de Béréa a été caractérisée par leur réponse électrique dans l'intervalle 10² - 10 puissance 8 Hz. Au moyen de traitements appropriés, la mouillabilité naturelle des matériaux a été modifiée afin d'obtenir deux séries différentes d'échantillons ayant respectivement de fortes mouillabilités à l'eau et à l'huile. Les échantillons ont été saturés à des degrés variés (pas plus de 40 % avec de l'eau permutée ou de la saumure. Les mesures ont montré que les réponses électriques des échantillons mouillables à l'eau ou mouillables à l'huile étaient nettement différentes et plus compliquées que celles prédites par deux modèles standard. En outre, on a pu constater que la dispersivité et la tangente de pertes constituent les paramètres les plus pertinents pour caractériser la mouillabilité des échantillons.

  15. Natural fracturing of rocks: application to the Ahnet basin (Algeria); Fracturation naturelle des roches: application au bassin de l`Ahnet (Algerie)

    Energy Technology Data Exchange (ETDEWEB)

    Badsi, M.


    In the Ahnet basin, the production seems to be unrelated to lithological variations in the reservoirs. In these large anticline structures, located in the central Ahnet basin, the presence of gas has been proven, but only a few production wells have been moderately successful. This inconsistency is probably related to the spatial distribution of fracturing throughout the reservoir. In order to investigate several hypothesis, we used several approach to solve problems posed by the interpreter: namely understanding the deformation process, predicting the fractured zones and building the discrete model of fracture network. This approach combines several methods, including sand box modelling, numerical modelling and Statistics rules, often related with fractal behaviour of faults families, have been used for extrapolating observations from seismic or from wells. The numerical tools and sand box analysis have allowed us to answer to the questions related to the formation of this large anticlines in the Ahnet basin and suggest a probable origin of the variation in the spatial distribution of natural fractures. The deterministic predictions of small-scale faults use probabilistic approaches for spatial interpolation assuming implicitly relationship between detected large faults and unresolved small faults. The statistical modelling is used to carry out analysis of the spatial variation of mean fracture attributes at the global scale (a few kilometers) and a 3D stochastic modelling of the fracture system at the local scale (a few ten of meters). (author) 139 refs.

  16. Analytical and clinical comparison of Elecsys syphilis (Roche(®)) - Architect syphilis TP and reformulated Architect syphilis TP (Abbott(®)) assay. (United States)

    De Keukeleire, Steven; Desmet, Stefanie; Lagrou, Katrien; Oosterlynck, Julie; Verhulst, Manon; Van Besien, Jessica; Saegeman, Veroniek; Reynders, Marijke


    The performance of Elecsys Syphilis was compared to Architect Syphilis TP and Reformulated Architect Syphilis TP. The overall sensitivity and specificity were 98.4% and 99.5%, 97.7% and 97.1%, and 99.2% and 99.7% respectively. The assays are comparable and considered adequate for syphilis screening.

  17. Experimental study of the effect of damage on rocks permeability; Etude experimentale de l'effet de l'endommagement sur la permeabilite des roches

    Energy Technology Data Exchange (ETDEWEB)

    Bounenni, A


    The effect of the cracking of rocks on their permeabilities arouses a growing interest by its consequences on the safety of radioactive waste disposal, and its applications to some problems of oil prospecting. This thesis is devoted to the experimental study of this phenomenon on some varieties of porous rocks. The adopted method consists in examining the evolution of the permeability with damage. Two varieties of clays, known as of the East and Gard varieties, were studied for applications to the problems of waste disposal, as well as a variety of chalk coming from the North Sea for oil applications. The major difficulties of the study of clays consist of a very low permeability and of the brittleness of these materials which make them difficult to create a diffuse damage or cracking in them. We however managed to show for example that the permeability of these materials can multiplied by a factor 100 for 4 per cent irreversible voluminal strain. The chalk is more porous and more permeable, and presents non difficulty for the measurement of the permeability. But the difficulty of creating a state of diffuse damage in this material remains. However, by ways of complex loading paths, we could in some cases make evolve the damage and the permeability and establish a phenomenological relation between these two quantities. Creation of crack in these material by thermal damage, using a microwave oven, proved to be a much simpler way and more promising than the mechanical way. The observation of the damaged clay samples by the electron microscope enabled us to determine the size scale of the microscopic cracks. On the damaged chalk samples, the acoustic velocity measurement made it possible to establish a simple relation between the permeability and the damage. (author)

  18. About the identification of behaviour law parameters of clayey rocks; Sur l'identification des parametres des lois de comportement des roches argileuses

    Energy Technology Data Exchange (ETDEWEB)

    Lecampion, B


    This work aims at developing identification methods for clayey rock parameters. These methods are necessary for the interpretation of the numerous data obtained at the ANDRA's Meuse/Haute-Marne underground laboratory. Two main rheological aspects have been considered: the poro-elastic behaviour and the elasto-visco-plastic behaviour. The first part of the study focusses on the poro-elastic parameters. Chapter 2 recalls the direct problem and discusses some important points of the identification inverse problem. Chapter 3 deals with the formulation of gradient calculation techniques for the linear poro-elastic case. The resolution using the finite-element method is discussed. The direct and associated state differentiation methods are validated for a 2D numerical example using the finite-element code Cast3M. The identification of poro-elastic coefficients of the Meuse/Haute-Marne argillaceous rocks is discussed in detail in chapter 4. The use of approximate semi-explicit solutions of the direct problems allows to obtain a fast identification method. The second part deals with the identification of elasto-visco-plastic parameters. The visco-plastic behaviour of Meuse/Haute-Marne rocks is discussed in chapter 5 and a visco-plastic model with nonlinear isotropic cold-drawing is proposed which allows to reproduce the tests. The parameters of this behaviour law are identified on a 1D creep test in drained conditions. Thus, the delayed deformations come from the poro-elastic and visco-plastic behaviour of the rock. It is shown that both phenomena can be separated. All poro-elasto-visco-plastic parameters are identified and a semi-explicit solution of the creep test is used. Chapter 6 presents an identification method of the elasto-visco-plastic parameters for the general case. The identification is equivalent to the minimization of a cost functional. The gradient of the functional is calculated by direct differentiation. The direct differentiation method is developed in Cast3M for the Norton-Hoff elasto-visco-plastic law. The identification of the parameters of this law on 1D and 2D configurations of underground facilities is then presented. Measurements are simulated using a direct calculation, eventually disturbed. The unicity and stability of this identification problem are discussed. In chapter 7, the method is applied to a real case: the Chamoise tunnel. 1D and 2D flat deformation modeling is used to solve the direct problem. The modeling hypotheses are justified and the obtained parameters allow to correctly reproduce the data. (J.S.)

  19. Roche Portable Blood Glucose Meter for Clinical Application and Quality Control%便携式血糖仪临床应用与质量控制

    Institute of Scientific and Technical Information of China (English)



    Diabetes is a lifelong disease is stil no cure at present, timing determination of glucose, strict control of blood glucose is the key to the treatment of diabetes melitus. Diabetes ideal blood glucose test is weekly 1~2 times, but generaly patients with no conditions to the hospital for frequent blood glucose testing, and the portable blood glucose meter provides great convenience for patients, the portable blood glucose meter for each ward monitoring blood glucose in patients with circadian changes and outpatient diabetes screening, increasingly the attention of medical workers and patients in this paper, Glucose meter in the clinical diagnosis of practical value in clinical application, quality assessment.%糖尿病是目前尚不能根治的终身性疾病,定时测定血糖值,严格控制血糖是糖尿病治疗的关键。糖尿病理想的血糖测试是每周1~2次,但一般患者没条件到医院作频繁的血糖测试,而便携式血糖仪为患者提供了很大的方便。便携式血糖仪因适合各病房昼夜监测患者血糖变化及门诊患者糖尿病筛查,日益受到医务工作者和患者的重视,本文从临床应用、质量检测评估方面阐述血糖仪在临床诊断的实用价值。

  20. Damage-induced permeability changes around underground excavations; Endommagement des roches argileuses et permeabilite induite au voisinage d'ouvrages souterrains

    Energy Technology Data Exchange (ETDEWEB)

    Coll, C


    The storage of nuclear waste in deep geological formations is now considered more and more as a potential solution. During excavation, a disturbed zone develops in which damaging can be important and which can lead eventually to the failure of the rock. Fluid flow and permeability in the rock mass can be significantly modified producing a possible security risk. Our work consisted in an experimental study of the hydro-mechanical coupling of two argillaceous rocks: Boom clay (Mol, Belgium) and Opalinus clay (Mont-Terri, Switzerland). Triaxial tests were performed in a saturated state to study the permeability evolution of both clays with isotropic and deviatoric stresses. Argillaceous rocks are geo-materials with complex behaviour governed by numerous coupled processes. Strong physico-chemical interactions between the fluid and the solid particles and their very low permeability required the modification of the experimental set up. Moreover, specific procedures were developed to measure permeability and to detect strain localisation in shear bands. We show that for Boom Clay, permeability is not significantly influenced by strain localisation. For Opalinus clay, fracturing can induce an increase of the permeability at low confining pressure. (author)

  1. Evaluation of an Upgraded Version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 Test for HIV-1 Load Quantification▿


    Damond, F.; Avettand-Fenoel, V.; Collin, G.; Roquebert, B.; Plantier, J. C.; Ganon, A.; Sizmann, D.; Babiel, R. (Rainer); Glaubitz, J.; Chaix, M. L.; Brun-Vezinet, F.; Descamps, D; Rouzioux, C


    We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated.

  2. Evaluation of an Upgraded Version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 Test for HIV-1 Load Quantification▿ (United States)

    Damond, F.; Avettand-Fenoel, V.; Collin, G.; Roquebert, B.; Plantier, J. C.; Ganon, A.; Sizmann, D.; Babiel, R.; Glaubitz, J.; Chaix, M. L.; Brun-Vezinet, F.; Descamps, D.; Rouzioux, C.


    We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated. PMID:20129964

  3. Evaluation of an upgraded version of the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test for HIV-1 load quantification. (United States)

    Damond, F; Avettand-Fenoel, V; Collin, G; Roquebert, B; Plantier, J C; Ganon, A; Sizmann, D; Babiel, R; Glaubitz, J; Chaix, M L; Brun-Vezinet, F; Descamps, D; Rouzioux, C


    We evaluated the performance of the prototype Cobas AmpliPrep/Cobas TaqMan HIV-1 test, version 2.0, using prospective and archived clinical samples initially underquantitated by the Cobas AmpliPrep/Cobas TaqMan HIV-1 test. The performance of the new test was significantly improved, and the majority of the underquantitation observed with the first-version test was eliminated.

  4. Prevalence of human papillomavirus in 5,072 consecutive cervical SurePath samples evaluated with the Roche cobas HPV real-time PCR assay.

    Directory of Open Access Journals (Sweden)

    Sarah Preisler

    Full Text Available New commercially available Human Papillomavirus (HPV assays need to be evaluated in a variety of cervical screening settings. Cobas HPV Test (cobas is a real-time PCR-based assay allowing for separate detection of HPV genotypes 16 and 18 and a bulk of 12 other high-risk genotypes. The aim of the present study, Horizon, was to assess the prevalence of high-risk HPV infections in an area with a high background risk of cervical cancer, where women aged 23-65 years are targeted for cervical screening. We collected 6,258 consecutive cervical samples from the largest cervical screening laboratory in Denmark serving the whole of Copenhagen. All samples were stored in SurePath media. In total, 5,072 samples were tested with cobas, Hybrid Capture 2 High Risk HPV DNA test (HC2 and liquid-based cytology. Of these, 27% tested positive on cobas. This proportion decreased by age, being 43% in women aged 23-29 years and 10% in women aged 60-65 years. HC2 assay was positive in 20% of samples, and cytology was abnormal (≥ atypical squamous cells of undetermined significance for 7% samples. When only samples without recent abnormalities were taken into account, 24% tested positive on cobas, 19% on HC2, and 5% had abnormal cytology. The proportion of positive cobas samples was higher than in the ATHENA trial. The age-standardized cobas positivity vs. cytology abnormality was 3.9 in our study and 1.7 in ATHENA. If in Copenhagen the presently used cytology would be replaced by cobas in women above age 30 years, an extra 11% of women would based on historical data be expected to have a positive cobas test without an underlying cervical intraepithelial lesion grade 3 or worse. Countries with a high prevalence of HPV infections should therefore proceed to primary HPV-based cervical screening with caution.

  5. Visualisation des propriétés capillaires des roches réservoir Visualizing the Capillary Properties of Reservoir Rocks

    Directory of Open Access Journals (Sweden)

    Zinszner B.


    Full Text Available Cet article décrit des expériences de drainage par centrifugation et d'imbibition par ascension capillaire réalisées avec des résines époxy colorées. Après polymérisation, l'observation des lames minces permet de localiser les fluides mouillants et non mouillants. Après avoir décrit les modes opératoires en insistant sur l'analyse des paramètres expérimentaux, on donne des exemples d'applications à la géologie de réservoir. Deux points sont développés : les études de perméabilités et les modèles de réservoir qui permettent d'étudier la répartition du fluide mouillant et des fractions déplaçables ou piégées du fluide non mouillant. This article describes drainage experiments by centrifuge method and imbibition by capillary rise performed with colored epoxy resins. After polymerization, analysis of thin sections serves to situate the wetting and nonwetting fluids. After describing the operating methods with em-phasis on the analysis of experimental parameters, the article gives examples of applications to reservoir geology. The following two points are developed : (i permeability investigations and (ii reservoir modelswhich can be used to analyze the distribution of the wetting fluid and the movable or trapped fractions of the nonwetting fluid

  6. Benefit of Hepatitis C Virus Core Antigen Assay in Prediction of Therapeutic Response to Interferon and Ribavirin Combination Therapy


    Takahashi, Masahiko; Saito, Hidetsugu; Higashimoto, Makiko; Atsukawa, Kazuhiro; Ishii, Hiromasa


    A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor test, version 2 (Amplicor M), to estimate the predictive benefit of a sustained viral response (SVR) and non-SVR in 44 genotype 1b patients treated with interferon (IFN) ...

  7. Survival of infants born to HIV-positive mothers, by feeding modality, in Rakai, Uganda.

    Directory of Open Access Journals (Sweden)

    Joseph Kagaayi

    Full Text Available BACKGROUND: Data comparing survival of formula-fed to breast-fed infants in programmatic settings are limited. We compared mortality and HIV-free of breast and formula-fed infants born to HIV-positive mothers in a program in rural, Rakai District Uganda. METHODOLOGY/PRINCIPAL FINDINGS: One hundred eighty two infants born to HIV-positive mothers were followed at one, six and twelve months postpartum. Mothers were given infant-feeding counseling and allowed to make informed choices as to whether to formula-feed or breast-feed. Eligible mothers and infants received antiretroviral therapy (ART if indicated. Mothers and their newborns received prophylaxis for prevention of mother-to-child HIV transmission (pMTCT if they were not receiving ART. Infant HIV infection was detected by PCR (Roche Amplicor 1.5 during the follow-up visits. Kaplan Meier time-to-event methods were used to compare mortality and HIV-free survival. The adjusted hazard ratio (Adjusted HR of infant HIV-free survival was estimated by Cox regression. Seventy-five infants (41% were formula-fed while 107 (59% were breast-fed. Exclusive breast-feeding was practiced by only 25% of breast-feeding women at one month postpartum. The cumulative 12-month probability of infant mortality was 18% (95% CI = 11%-29% among the formula-fed compared to 3% (95% CI = 1%-9% among the breast-fed infants (unadjusted hazard ratio (HR = 6.1(95% CI = 1.7-21.4, P-value < 0.01. There were no statistically significant differentials in HIV-free survival by feeding choice (86% in the formula-fed compared to 96% in breast-fed group (Adjusted RH = 2.8[95%CI = 0.67-11.7, P-value = 0.16] CONCLUSIONS/SIGNIFICANCE: Formula-feeding was associated with a higher risk of infant mortality than breastfeeding in this rural population. Our findings suggest that formula-feeding should be discouraged in similar African settings.

  8. Mechanical behaviour and rupture in clayey rocks studied by x-ray micro tomography; Comportement mecanique et rupture dans les roches argileuses etudies par micro tomographie a rayons X

    Energy Technology Data Exchange (ETDEWEB)

    Lenoir, N


    Within the framework of feasibility studies of underground repositories for radioactive waste, the study of permeability evolution with damage of the host layer is crucial. The goals of this work were: (i) to characterize experimentally the damage of two clayey rocks (BEAUCAIRE MARL and EAST SHALE) with x-ray micro tomography, (ii) to develop a high pressure triaxial set-up adapted to permeability measurement on very low permeability rocks.A number of original triaxial devices have been realised to characterize damage of clayey rocks, under deviatoric loading, with x-ray micro tomography on a synchrotron beamline at the ESRF (Grenoble). Localized damage and its evolution have been characterized at a fine scale (of order of ten microns). Digital image correlation techniques, extended to 3d images, have been used to measure incremental strain fields from tomographic images. we demonstrated that these techniques are very useful in the study of the localized damage of geo-materials and especially for the initiation. A high pressure triaxial device has been realised to measure permeability evolution of the east shale as a function of applied stress (isotropic and deviatoric). The particularity of this set-up is the small size of the test specimen (cylinder of 10 mm in diameter and 20 mm in height) which allows significant reduction of test duration. (author)

  9. NMR studies of the molecules dynamics to the solid-liquid interfaces: from graded porous materials to oil rocks; Etudes RMN de la dynamique des molecules aux interfaces solide-liquide: des materiaux poreux calibres aux roches petroliferes

    Energy Technology Data Exchange (ETDEWEB)

    Godefroy, S.


    Low field NMR relaxation for laboratory or in-situ applications provides critical information for oil recovery such as porosity, saturation, and permeability of rocks. In addition, pore size distribution and wettability can also be obtained in some cases. The technique relies on the measurement of proton longitudinal (T{sub 1}) or transverse (T{sub 2}) nuclear relaxation times. For better predictions, the surface micro-dynamics and the chemical properties of the liquids entrapped in the pore space are important and must be characterized. It is well known that the NMR relaxation is enhanced by the paramagnetic impurities at the pore surface but many other parameters influence the relaxation time distributions. These parameters are used to derive the petrophysical properties of the rocks. We propose here an original method to probe the dynamics of water and oil at the pore surface. In the present study, we used both nuclear relaxation at 2.2 MHz and field cycling Nuclear Magnetic Relaxation Dispersion (NMRD) techniques. We applied these two techniques to different kinds of water or oil saturated macroporous media (grain packings, outcrop and reservoir rocks with SiO{sub 2} or CaCO{sub 3} surfaces). We studied the dependence of NMR relaxation on pore size, magnetic field and temperature. Varying the pore size and the surface density of paramagnetic impurities of water saturated grain packings allowed experimental evidence for the two limiting regimes of the water relaxation in pores (surface- and diffusion-limited regimes). NMRD technique (evolution of 1/T{sub 1} with the magnetic field) allowed us to probe liquid surface dynamics in water or oil fully saturated grain packing, outcrop rocks or reservoir rocks (water- and oil-wet surfaces). We evidenced a two-dimensional molecular surface diffusion and directly estimated important parameters such as correlation times, residence times and molecular self-diffusion on the surface. Finally, we proved that the temperature dependence of T{sub 1} and T{sub 2} is directly related to the surface chemistry of the rocks. Such a dependence is clearly important for oil field in-situ measurements (well logging). (author)

  10. Geochemical modeling of water-gas-rock interactions. Application to mineral diagenesis in geologic reservoirs; Modelisation geochimique des interactions eau-gaz-roche. Application a la diagenese minerale dans les reservoirs geologiques

    Energy Technology Data Exchange (ETDEWEB)

    Bildstein, O.


    The Ph.D. report describes a conceptual and numerical model for simulating gas-water-rock interaction during mineral diagenesis of sediments. The main specific features of this model are the following: applicable to open systems, half-implicit resolution numerical method, feedback on the texture evolution (grain model), existence of a gas phase, oxido-reduction phenomena. (author) 217 refs.

  11. Composite microstructural anisotropies in reservoir rocks: consequences on elastic properties and relation with deformation; Anisotropies microstructurales composites dans les roches reservoir: consequences sur les proprietes elastiques et relation a la deformation

    Energy Technology Data Exchange (ETDEWEB)

    Louis, L.


    From diagenesis to tectonic stress induced deformation, rock microstructures always present some anisotropy associated with a preferential orientation, shape or spatial arrangement of its constituents. Considering the consequences anisotropy has on directional transport properties and compliance, as the geological history it carries, this approach has received a particular attention in numerous works. In this work, the microstructural features of various sedimentary rocks were investigated through direct observations and laboratory measurements in naturally deformed and undeformed blocks, samples being considered as effective media. All investigated samples were found to be anisotropic with respect to the physical properties we measured (i.e. ultrasonic P-wave velocity, magnetic susceptibility, electrical conductivity). Considering that P-wave velocities can be described by a second order tensor, we applied to the velocity data the same inversion procedure as the one routinely used in magnetic studies, which provided an efficient tool to estimate and compare these 3D anisotropies with respect to the original sample geographical position. In each case, we tried to identify as thoroughly as possible the microstructural source of the observed anisotropies, first by the mean of existing models, then through direct observations (optic and electronic microscopy). Depending on the rock investigated, anisotropy was found to be controlled by pore shape, intergranular contact distribution, preferentially oriented microcracks interacting with compaction pattern or pressure solution cleavages interacting with each other. The net result of this work is that P-wave velocity anisotropy can express the interaction between different microstructural features as well as their evolution during deformation. (author)

  12. An experimental study on mineral sequestration of CO{sub 2} in basics and ultra basics rocks; Etude experimentale des reactions de carbonatation minerale du CO{sub 2} dans les roches basiques et ultrabasiques

    Energy Technology Data Exchange (ETDEWEB)

    Dufaud, F


    The first part of the thesis is dedicated to dissolution data of siderite FeCO{sub 3} and magnetite Fe{sub 3}O{sub 4} which have been monitored in situ on the FAME beamline of the european synchrotron radiation facility in Grenoble. Iron in solution close to siderite single crystals is shown to be divalent hydrated. The small size of the experimentally investigated volume of solution (200 *400 micrometer and 3 mm height) allowed to work with single crystals in well defined geometries. No specific interaction was observed between iron (II) and dissolved inorganic carbon, suggesting that modelling siderite evolution under high CO{sub 2} pressures by using CO{sub 2}-less very acidic (pH 1-2) solutions is adequate. Using initial reaction rates, we get an activation energy for siderite dissolution of 62 kJ.mol{sup -1}, consistent with existing literature data. Such a value is suggestive of a mineral/solution interface mechanism.. Data from this study and from literature are consistent over a temperature range 25 C - 125 C and a pH range pH 1-7 with an empirical law: pk = pH + E{sub a}/(ln(10)*RT(K)) - log(S/V) - 10,5 where E{sub a} = 62 kJ.mol{sup -1} and S/V is the ratio between solid surface S and fluid volume V. A value of activation energy of 73.5 kJ.mol{sup -1} is obtained in the case of magnetite, also consistent with mineral/solution processes. The second and major part of the thesis work is the realization of analogical experiments for simulating carbonation of basic and ultra basic minerals. Experiments were carried out on consolidated rock cores at 90 C and 280 bar of CO{sub 2} (low temperature experiments) and on powders contained in metallic capsules at 400-500 C and 1000-1700 bars of CO{sub 2} (high temperature experiments). The rate of mineral storage of CO{sub 2} was defined as the molar ratio of solid carbonate formed over total CO{sub 2} injected. It is of about 1% in three months in low temperature experiments whereas it reaches several tens of percents per hour in high temperature experiments. In all cases, carbonation is shown to proceed according to dissolution/precipitation mechanisms. A quasi stoichiometric coupling is evidenced between carbonation and (proto) serpentinization in high-temperature experiments. Newly formed carbonates are mostly magnesite MgCO{sub 3} with Fe and Ca in solid solution. In low temperature samples, the silicates are covered with a thin silica layer and with carbonate spherules consisting of ankerite CaFe(CO{sub 3}){sub 2} - dolomite CaMg(CO{sub 3}){sub 2} - siderite cores surrounded by magnesite overgrowth. In CO{sub 2}-saturated water, peridotites are more reactive than serpentinite and basalts, in accordance with thermodynamic modelling whereas serpentinites are the most reactive in the supercritical CO{sub 2} phase, showing stronger reactivities in this latter phase than in CO{sub 2}-saturated water. In high temperature experiments, the rate of mineral storage is larger at 400 C than at 500 C, in agreement with thermodynamic modelling of the system. High water fugacities and high fluid salinities are shown to have a positive effect on mineral storage rates. Isotopic mass balance of carbon have evidenced that about 15% of the mineral storage consist of a reduced carbon phase, also identified by transmission electron microscopy as an ill-organized graphite phase. (author)

  13. Water-rock interaction under peri-glacial conditions: example of the secondary carbonates of the Broegger Peninsula (Spitzbergen); Interactions eaux-roches en milieu periglaciaire: exemple des carbonates secondaires de la presqu`ile de Broegger (Spitzberg)

    Energy Technology Data Exchange (ETDEWEB)

    Marlin, C.; Dever, L. [Paris-11 Univ., 91 - Orsay (France). Laboratoire d`Hydrologie et de Geochimie Isotopique


    Measurements of the isotopic and chemical contents of soil water and carbonates at different field sites in Spitzbergen were undertaken to study the precipitation conditions of soil secondary calcites under the current peri-glacial climate. A main experimental site (`cote 80`) has been established located on a fluvio-glacial terrasse at 80 m.a.s.l. near Ny Alesund (79 deg N, 12 deg. E). The active layer is at around 1.2 m depth on a continuous permafrost. The soil temperatures measured every 5 cm from the surface to the permafrost show that the freezing fronts move both the surface and permafrost, converging at around 0.6 m depth where the system is closed. During the beginning of the freezing period, the solute content increases in the residual water according to the distribution coefficient between water and ice. Calcite precipitation occurs in a second stage as indicated by the simultaneous decrease of the calcite saturation index and increase of the concentration of non-interactive elements. Chemical and isotopic ({sup 18}O, {sup 2}H, {sup 13}C et {sup 14}C) analyses have been made on the different samples with a mineralogical description of the carbonate coatings obtained by SEM and microprobe analyses. The isotopic values result from a mixing between recent calcites and `old` calcites. The recent calcites are probably in isotopic equilibrium with the present day solutions. The `old calcites` have precipitated under colder conditions than today. The low radiocarbon activities (10.2 to 24.8 pcm) of the `cote 80` site indicate that the `old calcites` have precipitated during the last interglacial period or an inter-stadial period of the Pleistocene. The good relationship between the carbon- 14 activity and the carbon- 13 content indicates that the beginning of the pedogenesis is not identical at all sites and is dependent on the timing of deglaciation and vulnerability of rocks to frost-weathering. (authors) 18 refs.

  14. Umbrella structure and channel-wall stoping in the Cambrian St. Roch Formation, Quebec Appalachians: significance for particle support mechanisms and turbulence development in hyper-concentrated sediment gravity flows (United States)

    Hesse, Reinhard; Fong, Christopher


    Umbrella structure is a newly recognized sedimentary structure associated with large platy clasts in resedimented boulder-bearing pebble conglomerate with a sandy matrix. It consists of a sand rim that lacks pebbles on parts or the entire underside of platy boulders, whereas on the upper side, pebbles are in direct contact with the boulders. The depositing processes were high- to hyper-concentrated sediment gravity flows in a submarine channel or canyon on the Cambrian continental slope of North America bordering the Iapetus Ocean. The structure occurs predominantly where clasts dip moderately in the down-current direction. Based on the association of the structure with slightly forward dipping slabs, it is proposed that these down-current dipping slabs may have been in the process of counter-clockwise rotation that was aborted and may have generated a pressure shadow on the underside enabling the inrush of fluid and the infiltration of sand into the anomalous low-pressure zone. The structure has implications for particle support mechanisms in high- to hyper-concentrated sedimentary gravity flows, in that it redirects attention to the much debated mechanism of dispersive pressure and alternatives. It provides an observable sediment structure that supports dispersive pressure which so far depended on experimental evidence and theoretical arguments alone. Vrolijk and Southard's (1997) concept of a `laminar sheared layer' is here for the first time interpreted as having an upward-moving `free-surface' layer effect during deposition from hyper-concentrated flows. Channel-wall stoping involves unlithified turbiditic spillover sand in the levee sediment of the canyon wall that was washed out by the upper diluted parts of the high-concentration flows coming down the channel and leaving a niche in the wall that was filled with coarser channel-axis facies by the same flow (or later flows) when its aggradation reached the level of the niche. The contact between turbidite and pebble conglomerate occurs now more than 2 m laterally into the exposed channel wall. Channel-wall stoping tracks turbulence development in hyper-concentrated gravity flows.

  15. Altération des biotites dans les roches cuprifères de Salobo A3, Serra dos Carajàs (Brésil).2. Association du cuivre aux phyllosilicates secondaires


    Toledo-Groke, M.C.; Parisot, Jean-Claude; Melfi, A.J.; Boulangé, Bruno


    Dans les gisements cuprifères de Salobo 3A (Serra dos Carajas - Brésil) les biotites saines sont totalement dépourvues de cuivres. Il est montré que dans les phyllosilicates secondaires issus de l'altération météorique de ces biotites la phase minéralogique la plus riche en cuivre est représentée par un produit appelé "biotite altérée". Dans le cas où se forme une smectite, celle-ci présente un enrichissement en cuivre moins important. La kaolinite issue de la transformation secondaire de ces...

  16. Smallpox and pan-Orthodox Virus Detection by Real-Time 3’-Minor Groove Binder TaqMan Assays Oil the Roche LightCycler and the Cepheid Smart Cycler Platforms (United States)


    Bacillus anthracis BA0068 Ames Sterne SPS 97.13.213 Bacillus cereus Bacillus coagulans Bacillus licheniformis Bacillus macerans Bacillus ...megaterium Bacillus polymyxa Bacillus sphaericus Bacillus stearothermophilus Bacillus subtilis subsp. niger Bacillus thuringiensis Bacillus popilliae...HA-MGB assay presented here has been used to monitor the viral load in monkey blood and tissues after infection with

  17. Evaluation of performances of VERSANT HCV RNA 1.0 assay (kPCR) and Roche COBAS AmpliPrep/COBAS TaqMan HCV test v2.0 at low level viremia. (United States)

    Mazzuti, Laura; Lozzi, Maria Antonietta; Riva, Elisabetta; Maida, Paola; Falasca, Francesca; Antonelli, Guido; Turriziani, Ombretta


    We assess the concordance between low level HCV values obtained using the VERSANT HCV RNA 1.0 Assay (kPCR) and COBAS AmpliPrep/COBAS TaqMan HCV Quantitative Test v2.0. The correlation between the values obtained by the two RT-PCR assays for samples with quantifiable HCV RNA levels revealed that viral load measured by kPCR significantly correlated with that of the CAP/CTM (R=0.644, PHCV triple therapy or interferon- free regimens. It is therefore recommended to monitor individual patients with the same test throughout treatment.

  18. Contribution to the modeling of the anisotropic damage and the variation of the permeability of the brittle rocks; Contribution a la modelisation de l'endommagement anisotrope et de la variation de la permeabilite des roches fragiles

    Energy Technology Data Exchange (ETDEWEB)

    Zhou, J


    This work deals with a modeling of the mechanical and hydro-mechanical behaviour of saturated rocks taking into account the variation of the permeability with damage. At first is established a function of the free enthalpy by a direct micro-mechanical approach in taking into account the distribution of the microcrack length. The opening of the closed microcracks due to the tangential gliding is taken into account because of the roughness of the cracks surfaces. This opening contributes directly to the volume expansion and to the variation of the macroscopic permeability of the material. A phenomenological approach with an approximation by a damage tensor of two order is shown too. Then, an extension of the model is proposed for describing the poro-mechanical behaviour of saturated rocks. The poro-mechanical answers in drained and undrained conditions of the Vosges sandstone have been studied. In the last part, a coupled model describing the behaviour of the coupling between the induced damage and the variation of the rocks permeability is proposed. (O.M.)

  19. Experimental study of very-low permeability rocks by the implementation of a precision permeameter; Etude experimentale des roches a tres faible permeabilite par la mise en oeuvre d'un permeametre de precision

    Energy Technology Data Exchange (ETDEWEB)

    Larive, E


    The measurement of fluid flow through 'tight' rocks is important to provide a better understanding of physical processes involved in several industrial and natural problems. These include deep nuclear waste repositories, management of aquifers, gas, petroleum or geothermal reservoirs, or earthquakes prevention. The major part of this work consisted of the design, construction and use of an elaborate experimental apparatus allowing laboratory permeability measurements (fluid flow) of very low permeability rocks, on samples at a centimetric scale, to constrain their hydraulic behaviour at realistic in-situ conditions.The accuracy permeameter allows the use of several measurement methods, the steady-state flow method, the transient pulse method and the sinusoidal pore pressure oscillation method. Measurements were made with the pore pressure oscillation method, using different waveform periods, at several pore and confining pressure conditions on different materials. The permeability of one natural standard, Westerly granite, and an artificial one, a micro-porous cement, were measured and results obtained agreed with previous measurements made on these materials showing the reliability of the permeameter. A study of a Yorkshire sandstone shows a relationship between rock microstructure, permeability anisotropy and thermal cracking. Microstructure, porosity and permeability concepts, and laboratory permeability measurements specifications are presented, the permeameter is described, and then permeability results obtained on the investigated materials are reported. (author)

  20. Comparative evaluation of Roche Aurora FLOW, Becton and Dickinson Viper system, and Dynex DS2 for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis in various clinical specimens. (United States)

    Vahidnia, Ali; Costa, Sandra; Veenings, Sanne; Tuin, Hellen; van Loon, Linda; Bliekendaal, Harry


    The purpose of this study is to introduce a high-throughput system, Aurora FLOW, for the simultaneous detection of 3 clinically relevant pathogens of sexually transmitted infections. Comparative evaluation with other systems revealed an overall concordance of 97.9% for Chlamydia trachomatis and comparable performance for Neisseria gonorrhoeae and Trichomonas vaginalis.

  1. Efficacité de la roche phosphatée de Matongo au travers d'un compostage sur une culture de pomme de terre sur un sol acide de Rabiro (Burundi

    Directory of Open Access Journals (Sweden)

    Van den Berghe, C.


    Full Text Available Efficiency of phosphatic rock from Matongo applied in the composting process on potatoes on an acid soil of Rabiro. In the frame of the Cooperation between the CVHA (Cultures Vivrieres de Haute Altitute Project and the Program of Fertilisation of the Agro-systems on Altitude (FAVA of the Faculty of Agricultural Sciences in Burundi, the local phosphatic rock from Matongo has been compared to diammonium-phosphate when added in the composting process. The field trials with potatoes have shown that both phosphate sources have the same fertilizing value when the enriched compost was applied at the dose of 20 t/ha. It is very interesting from agricultural and economical viewpoint to use this phosphatic rock in combination with compost.

  2. Robaszynski, F. et Guyétant, G., coordination (2009). Des roches aux paysages dans le Nord - Pas-de-Calais. Richesse de notre patrimoine géologique


    Pastre, Jean-François


    Cet ouvrage grand public propose la découverte de près de 450 millions d’années d’histoire géologique du Nord - Pas-de-Calais. Du Boulonnais à l’Avesnois, en passant par le bassin minier, l’Artois, la Flandre et les plaines maritimes, l’étude de vingt-cinq sites emblématiques du patrimoine géologique régional permet à différents spécialistes d’exposer les principales étapes de son évolution. Après la présentation de quelques bases pour aborder la géologie du Nord – Pas-de-Calais, un premier ...

  3. Simulation of anions diffusion in Callovo-Oxfordian argillite by description of the rock microstructure; Modelisation de la diffusion des anions dans l'argilite du Callovo-oxfordien par description de la microstructure de la roche

    Energy Technology Data Exchange (ETDEWEB)

    Diaz, N. [CEA Saclay, Dept. de Physico-Chimie (DEN/DANS/DPC/SECR), 91 - Gif sur Yvette (France)


    The Callovo-Oxfordian argillite has been proposed as host rock for deep underground radioactive wastes storage. The aim of this study is to determine the report of effective diffusion coefficients De(anion)/De(water) at the micrometer scale considering the spatial distribution of minerals in the rock. (A.L.B.)

  4. Development of a X-ray micro-tomograph and its application to reservoir rocks characterization; Developpement d`un microtomographe X et application a la caracterisation des roches reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira de Paiva, R.


    We describe the construction and application to studies in three dimensions of a laboratory micro-tomograph for the characterisation of heterogeneous solids at the scale of a few microns. The system is based on an electron microprobe and a two dimensional X-ray detector. The use of a low beam divergence for image acquisition allows use of simple and rapid reconstruction software whilst retaining reasonable acquisition times. Spatial resolutions of better than 3 microns in radiography and 10 microns in tomography are obtained. The applications of microtomography in the petroleum industry are illustrated by the study of fibre orientation in polymer composites, of the distribution of minerals and pore space in reservoir rocks, and of the interaction of salt water with a model porous medium. A correction for X-ray beam hardening is described and used to obtain improved discrimination of the phases present in the sample. In the case of a North Sea reservoir rock we show the possibility to distinguish quartz, feldspar and in certain zone kaolinite. The representativeness of the tomographic reconstruction is demonstrated by comparing the surface of the reconstructed specimen with corresponding images obtained in scanning electron microscopy. (author). 58 refs., 10 tabs., 71 photos.

  5. Feasibility study for the installation of a small hydro electric power plant; Etude de faisabilite. Petite centrale hydro-electrique au lieu dit 'Sous les Roches' a Sonceboz

    Energy Technology Data Exchange (ETDEWEB)

    Tissot, N. [MHyLab, Mini-Hydraulics Laboratory, Montcherand (Switzerland); Hausmann, H. [Hans Hausmann, Bevilard (Switzerland)


    This report for the Swiss Federal Office of Energy presents a technical, economical and ecological analysis of the feasibility of a small hydroelectric power plant at Sonceboz, in the Swiss Jura mountains. The power of the planed plant would typically be 500 kW for a water head of about 10 m. The study shows that, compared to one single turbine, a pair of Kaplan type turbines would be operational over a larger range of the river's water flow rate. This solution would be a little bit more costly but offer more flexibility for maintenance. Two maximum water flow rates are considered, 4.5 and 6 m{sup 3}/s respectively. According to the economical study, done with current electric kWh prices, both configurations are viable while the largest flow rate leads to a larger profit. The report is rounded up by a sensitivity analysis considering variations in annual power generation, electro-mechanical and construction cost as well as turbine efficiency. It indicates that this last factor could be crucial for the overall profitability. [French] Ce rapport est le resultat detaille de l'analyse technico-economique et ecologique de faisabilite de la realisation d'une petite centrale hydroelectrique basse chute qui pourrait exploiter une denivellation d'environ 10 m pour une puissance de l'ordre de 500 kW pres de Sonceboz dans le Jura suisse. L'etude montre que le couplage de deux turbines Kaplan offrirait une possibilite de fonctionnement sur une plus grande plage de flux ainsi qu'une plus grande flexibilite de maintenance, ceci pour un cout legerement superieur a l'option a une seule turbine. Les debits de 4.5 et 6 m{sup 3}/s sont consideres. L'etude economique montre que le debit de 6 m{sup 3}/s est plus rentable au prix de vente actuel du kWh. L'article se termine par une analyse de sensibilite sur les incertitudes concernant la production annuelle, les prix de l'electromecanique et du genie civil et le rendement des turbines.

  6. Detecção do DNA de Chlamydia trachomatis em espondiloartropatias e artrite reumatóide Detection of Chlamydia trachomatis DNA in spondyloarthropathies and rheumatoid arthritis

    Directory of Open Access Journals (Sweden)

    Rafael Navarrete Fernandez


    Full Text Available Chlamydia trachomatis é a bactéria responsável pela doença sexualmente transmissível mais prevalente no mundo. A maioria das infecções em homens e mulheres é assintomática e, quando não diagnosticada e tratada, pode causar artrite e complicações relacionadas ao aparelho reprodutor feminino. OBJETIVO: pesquisar o DNA de C. trachomatis no líquido sinovial e urina de pacientes com espondiloartropatias e artrite reumatóide (AR, avaliar a presença de anticorpos séricos IgG e IgM anti-C. trachomatis nesses dois grupos de doenças e identificar o antígeno HLA-B27 em pacientes com espondiloartropatias. MÉTODOS: a população do estudo consistiu em 15 pacientes com espondiloartropatias: nove com espondiloartropatia indiferenciada (EI e seis com artrite reativa (ARe (grupo I e 15 pacientes com AR (grupo II. O DNA clamidial foi pesquisado em amostras de líquido sinovial e urina de todos os pacientes, empregando-se a PCR (Amplicor Roche, Suíça. Os anticorpos IgG e IgM anticlamidiais foram quantificados por imunofluorescência indireta (IFI, enquanto o HLA-B27 foi tipado em 15 pacientes do grupo I por citometria de fluxo. RESULTADOS: o DNA da C. trachomatis foi evidenciado apenas em uma amostra de líquido sinovial do grupo I (6,7%, sendo o paciente portador de ARe. Em dois pacientes com AR, o DNA clamidial foi identificado na urina (13,3%. Os anticorpos IgG anticlamidiais estavam presentes em oito pacientes da população estudada, três do grupo I (20% e cinco do grupo II (33,3%. O maior título desse anticorpo (1/256 associou-se com a presença do DNA clamidial na urina de um paciente do grupo II. O anticorpo IgM não foi detectado em nenhuma amostra dos dois grupos. O antígeno HLA-B27 foi positivo em quatro indivíduos do grupo II (26,7% e sua presença relacionou-se com sacroileíte. CONCLUSÕES: os resultados deste estudo indicam que em pacientes com diagnóstico de espondiloartropatias e artrite reumatóide, com quadro articular

  7. Trachoma prevalence and associated risk factors in the gambia and Tanzania: baseline results of a cluster randomised controlled trial.

    Directory of Open Access Journals (Sweden)

    Emma M Harding-Esch

    Full Text Available BACKGROUND: Blinding trachoma, caused by ocular infection with Chlamydia trachomatis, is targeted for global elimination by 2020. Knowledge of risk factors can help target control interventions. METHODOLOGY/PRINCIPAL FINDINGS: As part of a cluster randomised controlled trial, we assessed the baseline prevalence of, and risk factors for, active trachoma and ocular C. trachomatis infection in randomly selected children aged 0-5 years from 48 Gambian and 36 Tanzanian communities. Both children's eyes were examined according to the World Health Organization (WHO simplified grading system, and an ocular swab was taken from each child's right eye and processed by Amplicor polymerase chain reaction to test for the presence of C. trachomatis DNA. Prevalence of active trachoma was 6.7% (335/5033 in The Gambia and 32.3% (1008/3122 in Tanzania. The countries' corresponding Amplicor positive prevalences were 0.8% and 21.9%. After adjustment, risk factors for follicular trachoma (TF in both countries were ocular or nasal discharge, a low level of household head education, and being aged ≥ 1 year. Additional risk factors in Tanzania were flies on the child's face, being Amplicor positive, and crowding (the number of children per household. The risk factors for being Amplicor positive in Tanzania were similar to those for TF, with the exclusion of flies and crowding. In The Gambia, only ocular discharge was associated with being Amplicor positive. CONCLUSIONS/SIGNIFICANCE: These results indicate that although the prevalence of active trachoma and Amplicor positives were very different between the two countries, the risk factors for active trachoma were similar but those for being Amplicor positive were different. The lack of an association between being Amplicor positive and TF in The Gambia highlights the poor correlation between the presence of trachoma clinical signs and evidence of C. trachomatis infection in this setting. Only ocular discharge was

  8. Ravimid, risk, raha & tervis / Inge Pitsner

    Index Scriptorium Estoniae

    Pitsner, Inge


    Ravimifirma Roche Eesti OÜ tegevjuht Kadri Mägi selgitab ravimiäri toimemehhanisme ning põhjuseid, miks ravimitööstus on riskantne ja kallis valdkond. Lisa: Mis on farmakoökonoomika?; Roche Eesti kontor

  9. Febrile Neutropenia Risk Assessment and Granulocyte-Colony Stimulating Factor Support in Patients with Diffuse Large B Cell Lymphoma Receiving R-CHOP Regimens

    DEFF Research Database (Denmark)

    Salar, Antonio; Haioun, Corinne; Rossi, Francesca Gaia;


    Disclosures: Salar: Amgen: Honoraria. Haioun: Roche: Research Funding; Amgen: Other, Research Funding; Celgene: Other, Research Funding; Mundipharma: Research Funding. Pettengell: Amgen: Honoraria, Speakers Bureau; Roche: Honoraria, Speakers Bureau; Bayer: Honoraria, Speakers Bureau. Jaeger: Amgen: Research...

  10. HBV and neurological impairment in HIV-infected patients

    Directory of Open Access Journals (Sweden)

    L Manolescu


    Full Text Available Objective: HIV can affect CNS in early stages of disease and determine neurological impairment. HBV DNA was found in CSF of HIV co-infected patients, but little is known about the neurotropic character of this virus. Here we assessed the degree of association between HBV infection and neurological impairment in a large cohort of long-term survivors, HIV-infected patients that experienced multiple therapeutic schemes over time. Methods: A total of 462 HIV-1-infected patients were retrospectively followed up for 10 years for HBV infection and neurological impairment. The patients were tested for immune (flow cytometry and virological parameters of HIV infection (Roche Amplicor, version 1.5/ COBAS AmpliPrep/COBAS TaqMan HIV-1 test and for HBV infection markers (HBsAg, anti HBc: Murex Biotech ELISA tests. Many of these patients have experienced between one and six regimens such as: 2 NRTIs, 3 NRTIs, 2 NRTIs+1 NNRTI, 1 NRTI+1 NNRTI+1 PI, 2 NRTIs+2 PIs. Results: After 10 years 29.87% of the patients presented neurological impairment. Out of them 56.52% were HBV-infected. The prevalence of HIV encephalopathy (HE in our studied cohort was 22.7% and 50.4% of these patients were HBV-infected. The median HIV diagnosis age was 7 and the median age of HE diagnosis was 10. In order to establish a possible correlation between HBV infection and HE we first reviewed and excluded the main risk factors associated with HE at the moment of diagnosis: low weight, anemia, constitutional symptoms, low CD4+count, high plasma HIV-RNA load. No patient was infected with HCV. The groups of patients that presented HE and HBsAg and HE without HBsAg were balanced regarding sex, number of deceased patients, number of class C3 patients, but the patients in first group presented lower CD4 values at HE diagnosis vs patients from second group 2: 44.5 vs 95 cells/µL, p=0.3; lower nadir CD4 count: 38 vs 51 cell/µL, p=0.1; and slightly higher HIV viral load: 5.2 vs 5 log10 copies

  11. Hepatitis B virus markers in anti-HBc only positive individuals. (United States)

    Weber, B; Melchior, W; Gehrke, R; Doerr, H W; Berger, A; Rabenau, H


    Isolated reactivity to hepatitis B virus (HBV) core antigen (anti-HBc) is observed relatively frequently in immunocompromised individuals, intravenous drug abusers (IVDA), and in the presence of HCV infection. The reason for the lack of HBsAg is not clear. The aim of the present study was to investigate which factors (genetic variability of S gene, low-level HBsAg, and immune complexes may be responsible for the failure of HBsAg detection with commercial HBsAg screening assays. Dilution series of two recombinant HBsAg escape mutants and dilutions of serum samples from chronic HBV carriers with multiple insertions in the a determinant and different HBsAg subtypes were tested with a highly sensitive assay that detects wild-type HBsAg (Elecsys HBsAg, Roche Diagnostics, Penzberg, Germany) and two assays that detect HBV wild-type and escape mutants (Murex HBsAg Version 3, Murex and Enzygnost HBsAg 5.0, Dade Behring, Marburg, Germany). Elecsys HBsAg showed in comparison to Murex HBsAg Version 3 and Enzygnost HBsAg 5.0 a reduced sensitivity for escape mutant detection. On the other hand, the best performance for HBsAg subtype detection was obtained with Elecsys HBsAg. In the second part of the study, a selected panel of isolated anti-HBc reactive (n = 104) serum samples (AxSYM Core) was submitted to testing by Elecsys HBsAg, Murex HBsAg Version 3, Enzygnost HBsAg 5.0, and HBsAg detection after immune complex dissociation (ICD) and anti-HBs determination with two different assays (AxSYM Ausab and Elecsys Anti-HBs). To assess the specificity of anti-HBc test results, all the samples were tested by a second anti-HBc assay (Elecsys Anti-HBc). Quantitative HBV DNA detection was undertaken with a commercially available HBV PCR assay (Amplicor HBV Monitor). HCV infection was present in 65.4% of anti-HBc only reactive individuals. Five AxSYM Core positive samples were negative by Elecsys Anti-HBc. Overall, 15 (14.4%) AxSYM Ausab negative samples gave positive results with Elecsys

  12. Benefit of hepatitis C virus core antigen assay in prediction of therapeutic response to interferon and ribavirin combination therapy. (United States)

    Takahashi, Masahiko; Saito, Hidetsugu; Higashimoto, Makiko; Atsukawa, Kazuhiro; Ishii, Hiromasa


    A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor test, version 2 (Amplicor M), to estimate the predictive benefit of a sustained viral response (SVR) and non-SVR in 44 genotype 1b patients treated with interferon (IFN) and ribavirin. HCV core Ag negativity could predict SVR on day 1 (sensitivity = 100%, specificity = 85.0%, accuracy = 86.4%), whereas RNA negativity could predict SVR on day 7 (sensitivity = 100%, specificity = 87.2%, accuracy = 88.6%). None of the patients who had detectable serum core Ag or RNA on day 14 achieved SVR (specificity = 100%). The predictive accuracy on day 14 was higher by RNA negativity (93.2%) than that by core Ag negativity (75.0%). The combined predictive criterion of both viral load decline during the first 24 h and basal viral load was also predictive for SVR; the sensitivities of Lumipulse-Ag and Amplicor-M were 45.5 and 47.6%, respectively, and the specificity was 100%. Amplicor-M had better predictive accuracy than Lumipulse-Ag in 2-week disappearance tests because it had better sensitivity. On the other hand, estimates of kinetic parameters were similar regardless of the detection method. Although the correlations between Lumipulse-Ag and Amplicor-M were good both before and 24 h after IFN administration, HCV core Ag seemed to be relatively lower 24 h after IFN administration than before administration. Lumipulse-Ag seems to be useful for detecting the HCV concentration during IFN therapy; however, we still need to understand the characteristics of the assay.

  13. Relations entre les types de dépôts évaporitiques et la présence de couches riches en matière organique (roches-mères potentielles Relationship Between Different Types of Evaporitic Deposits and the Occurrence of Organic-Rich Layers (Potential Source Rocks

    Directory of Open Access Journals (Sweden)

    Busson G.


    Full Text Available La fertilité exceptionnelle des eaux salées est confirmée par des études récentes sur l'Actuel (marais salants de Méditerranée occidentale. Le benthos est représenté par des mollusques, foraminifères, ostracodes et surtout des cyanophycées et des populations bactériennes ; le plancton par le microphytoplancton (Dunaliella, diatomées, etc, par le zooplancton (flagellés, Arternia salina et par de très nombreuses bactéries hétérotrophes. Si les espèces sont d'autant plus rares que la salinité est élevée, la prolifération des formes adaptées peut atteindre des valeurs supérieures à celles réalisées dans la plupart des autres milieux. On rappelle ensuite l'efficacité des systèmes d'eaux stratifiées pour la conservation de la matière organique originellement élaborée dans l'eau photique et oxygénée. Ces systèmes peuvent s'accompagner de proliférations bactériennes photosynthétiques exposées à des mortalités en masse pouvant être responsables de véritables lamines organiques. Dans les évaporites de plates-formes où la ségrégation des salinités et des dépôts a été synchrone et latérale, la tranche d'eau a dû être faible et, de ce fait, peu favorable à l'établissement de systèmes d'eaux stratifiées et surtout à leur pérennité géologique. Ces accumulations s'avèrent donc en général pauvres en matière organique, de même qu'elles ne s'accompagnent pas d'appareils récifaux abondants. Dans les évaporites de centre de cuvette, les dépôts sont imputables à une succession de phases de salinité croissante dans le temps : calcaires sur les zones hautes, contemporains de minces couches à matière organique dans les dépressions ; sulfate de calcium sur les aires d'épaisseur moyenne pouvant être encore contemporain de couche à matière organique dans les aires déprimées ; enfin, remplissage par la halite des zones centrales profondes et déprimées. A ces phases successives, se superposent - au moins au cours de la formation des carbonates, puis des sulfates - des gradients de salinité verticaux (système d'eaux stratifiées. Cartographiquement, ces dépôts ont une polarité salin croissant centripète . Quand elles sont situées en contexte carbonaté, ces évaporites de centre de cuvette s'accompagnent fréquemment d'appareils récifaux pouvant fourmiller dans toute une ceinture annulaire (cf. Salina du Silurien du Michigan. Grâce aux minces couches à matière organique des zones déprimées, grâce aux appareils récifaux et grâce à la couverture salifère, ces bassins ont souvent une certaine prolificité en hydrocarbures. Le troisième type correspond aux évaporites de marge de bassin. Dans ce dispositif, le centre du bassin est resté affamé - voire insatiable - pendant de longues périodes. La sédimentation y est restée marine, souvent caractérisée par la seule présence du plancton et du necton et par une richesse plus ou moins permanente en matière organique. Autour de la cuvette s'organisent, sur des plates-formes marginales parfois fort étendues, des appareils construits (bancs ou récifs, puis des faciès lagunaires, carbonatés et évaporitiques et enfin des détritiques continentaux. Les dépôts montrent une polarité salin croissant centrifuge irréductiblement opposée à la polarité inverse des dépôts de centre de cuvette. Le déficit en les sels les plus solubles est remarquable voire considérable, impliquant le reflux de saumures en profondeur à partir de ces annexes évaporatoires qu'ont dû constituer ces plates-formes d'arrière-récifs. L'appel d'eau à partir de ces annexes évaporatoires n'a pu qu'amener un afflux permanent de nutriments dans tout le bassin. Le reflux des saumures quant à lui a favorisé la pérennité d'un système à deux corps d'eau. Grâce à cet ajustement et à la pérennité du système, la prolificité de tels bassins en matière organique a pu être extrêmement importante. Les évaporites de marge de bassin avec couches à matière organique au centre correspondent à une période prolongée. C'est l'essentiel de la vie du bassin. Le remplissage de la cuvette centrale par des évaporites de centre de bassin peut être un épisode bref et qui peut entraîner la disparition même du bassin. Les évaporites de plate-forme s'étalant indifféremment sur l'ancien domaine du bassin comme sur celui des anciennes plates-formes marginales peuvent être l'aube d'un nouveau cycle sédimentaire, indifférent au passé. The extraordinary fertility of saline waters has been confirmed by recent studies of salterns in the western Mediterranean. The benthos contains mollusks, foraminifers, ostracodes and especially Cyanophyceae and bacterial populations. Plankton includes microphytoplankton (Dunaliella, diatoms, etc. , zooplankton (flagellates, Artemia salina and numerous heterotrophic bacteria. Where diversity is low when salinity is high, the proliferation of well adapted forms can be greater than the productivity levels observed in most other environments. The effectiveness of stratified water bodies for the preservation of organic matter originally produced in photic and oxygenated water is brought out. Such stratified systems may be accompanied by the proliferation of photosynthetic bacteria that are exposed to sporadic mass mortality, resulting in the formation of organic laminae at the bottom. In shelf (or epeiric evaporites, where the segregation of salinities and deposits has been synchronous and lateral, the water depth must have been shallow and hence unsuitable for the formation of stratified water bodies and especially for their geological duration. Such accumulations thus generally have a low organic content, and they also do not have abundant reef systems. In basin-center evaporites, the deposits are attributed to a succession of phases of increasing salinity in time, i. e. limestone in high areas, contemporaneous with thin organic-rich layers in low areas (hollows, calcium sulfate in medium-thickness areas which may also be contemporaneous with organicrich layers in low areas, followed by infilling by halite in deep and low-lying central areas. Superposed on these successive phases - at least during the formation of carbonates then sulfates - are vertical salinity gradients (stratified water system. When mapped, such deposits have a centripetal increasing saline polarity. When they are situated in a carbonate context, such basin-center evaporites are often associated with reef systems that may have thrived as a ring-like belt (cf. Silurian Salina, Michigan. Because of the thin organic-rich layers in low-lying areas, reef systems and the salt overburden, such basins are often prolific with hydrocarbons. The third type of evaporitic deposits is represented by basin-margin evaporites. In such a system, the basin center remains starved - even insatiable - for long periods of time. Sedimentation remains marine, often characterized by the presence of plankton and nekton alone and by a more or less constantly high organic content. Around the basin, marginal shelves are built and are even sometimes quite wide, accompagnied by built-up systems (banks or reefs, then by lagoonal, carbonate and evaporitic facies, and finally by continental detritals. The deposits have a centrifugal increasing saline polarity that is irreducibly opposed to the reverse polarity of the basin-center deposits. The deficit in the most soluble salts is significant, even considerable, involving brine outflow downward from these evaporitic appendages that must have been formed by the back-reef shelves. The inflow of water from these evaporitic appendages can only be constantly accompanied by nutrients throughout the entire basin. The outflow of brines enhances the continuing existence of a system with two water bodies. Because of this adjustment and the durable nature of the system, the prolificity of such basins in organic matter may have been extremely high. The basin margin evaporites with organic-rich in the center indicate that the process was a long one. This is the main life of the basin. The filling of the central part by basin-center evaporites may be a brief episode and may even result in the very disappearance of the basin. The shelf evaporites spreading on both the former basin area and the former marginal shelves may be the start of new sedimentary cycle without any connection to the past.

  14. Experimental study of chemical-mechanical coupling during percolation of reactive fluid through rocks under stress, in the context of the CO{sub 2} geological sequestration; Etude experimentale du couplage chimie-mecanique lors de la percolation d'un fluide reactif dans des roches sous contrainte, dans le contexte de la sequestration geologique du CO{sub 2}

    Energy Technology Data Exchange (ETDEWEB)

    Le Guen, Y


    CO{sub 2} injection into geological repositories will induce chemical and mechanical instabilities. The study of these instabilities is based on experimental deformation of natural rock samples under stress, in the presence of fluids containing, or not, dissolved CO{sub 2}. Triaxial cells used for the experiments permitted an independent control and measurement of stress, temperature, fluid pressure and composition. Vertical strains were measured during several months, with a resolution of 1.10{sup -12} s{sup -1} on the strain rate. Simultaneously, fluids were analysed in order to quantify fluid-rock interactions. For limestone samples, percolation of CO{sub 2}-rich fluids increases strain rate by a factor 1.7 up to 5; on the other hand, sandstone deformation remained almost the same. Increase in strain rate with limestone samples was explained by injected water acidification by the CO{sub 2} which increases rock solubility and reaction kinetics. On the opposite, small effect of CO{sub 2} on quartz explains the absence of deformation. X-ray observations confirmed the importance of rock composition and structure on the porosity evolution. Numerical simulations of rock elastic properties showed increasing shear stress into the sample. Measured deformation showed an evolution of reservoir rocks mechanical properties. It was interpreted as the consequence of pressure solution mechanisms both at grains contacts and on grain free surfaces. (author)

  15. Modelling the effective diffusion coefficient of anions in Callovo-Oxfordian argillite knowing the microstructure of the rock; Modelisation predictive de la migration des anions par description de la microstructure de la roche: application a l'argilite du Callovo-Oxfordien

    Energy Technology Data Exchange (ETDEWEB)

    Diaz, N.


    After having presented the issue of radioactive waste storage, the concept of geological storage and its application in the Meuse/Haute-Marne underground laboratory, and described the Callovo-Oxfordian geological formation and the argillite transport properties, this research thesis aims at developing a prediction of these properties at a macroscopic scale for water and anions. A first part presents the different experimental means implemented to acquire the diffusion coefficients for the studied materials (Callovo-Oxfordian argillite and purified Puy illite), and the spatial organisation of minerals by LIBS probe-based mapping to highlight a relationship between rock microstructure and its transport macroscopic properties. The next part presents the models which have been developed at the nanometer and micrometre scale to predict the diffusion coefficients. Experimental results are then compared with computed values

  16. Study of the reactive processes during CO{sub 2} injection into sedimentary reservoirs: Experimental quantification of the processes at meso-scale; Quantification experimentale a l'echelle mesoscopique des processus reactionnels dans le cadre de l'injection de CO{sub 2} dans des roches carbonatees et silicatees

    Energy Technology Data Exchange (ETDEWEB)

    Luquot, L.


    In order to minimize CO{sub 2} atmospheric concentration, a solution consists in sequestrating CO{sub 2} in geological reservoirs. To estimate long term risks, it is necessary to quantify the couplings between reaction processes as well as structural and hydrodynamical modifications. We realised two experimental benches enabling injecting CO{sub 2}-enriched-brine in conditions corresponding to in situ storage (T {<=} 200 C and P {<=} 200 bar) and developed an experimental protocol using X-Ray microtomography and fluid and rock analyses in order to measure the variations of physical and chemical parameters. The study of carbonated reservoirs near the injection well, allows quantifying different k-phi relationships depending on the dissolution processes and triggered by the local fluid chemical composition and initials conditions. Away from the injection well, we observe carbonate precipitation decreasing the permeability. The study of fractured cap-rock samples shows that alternative percolation of CO{sub 2}-enriched-brine and CO{sub 2} gas increases the fracture permeability. The study of silicated rocks indicates carbonate precipitation in zeolite sandstone and sintered dunite grains. Nevertheless, in zeolite sandstone we also observe the precipitation of clay particles located in the fluid pathways which decrease strongly the permeability. (author)

  17. From Injectivity to Integrity Studies of CO{sub 2} Geological Storage Chemical Alteration Effects on Carbonates Petrophysical and Geomechanical Properties; Caracterisation de l'injectivite et de l'integrite d'un stockage geologique de CO{sub 2}: Effets d'une alteration chimique sur les proprietes petrophysiques et geomecaniques des roches carbonatees

    Energy Technology Data Exchange (ETDEWEB)

    Bemer, E.; Lombard, J.M. [Institut francais du petrole, IFP, 92 - Rueil-Malmaison (France)


    The technical and economical success of a CO{sub 2} geological storage project requires the preservation of the site injectivity and integrity properties over its lifetime. Unlike conventional hydrocarbon gas injection, CO{sub 2} injection implies geochemical reactions between the reactive brine and the in situ formations (reservoir and cap rock) leading to modifications of their petrophysical and geomechanical properties. This paper underlines the experimental difficulties raised by the low permeability of samples representative either of the cap rock itself or at least of transition zones between the reservoir and the effective cap rock. Acidification effects induced by CO{sub 2} injection have been studied using an experimental procedure of chemical alteration, which ensures a homogeneous dissolution pattern throughout the rock sample and especially avoids any worm-holing process that would lead to erroneous measurements at the core scale. Porosity, permeability and geomechanical properties of outcrop and field carbonate samples of various permeability levels have been measured under their native state and different levels of alteration. The present work has been conducted within the framework of ANR GeoCarbone-INJECTIVITY and GeoCarbone-INTEGRITY projects. Each experimental step: chemical alteration, petrophysical measurements and geomechanical testing, is considered from the point of view of injectivity and integrity issues. The obtained experimental data show clear trends of chemically induced mechanical weakening. (authors)

  18. Temperature effect on the poro-mechanical or hydraulic behaviour of a carbonated rock and a mortar: experimental studies; Influence de la temperature sur le comportement poromecanique ou hydraulique d'une roche carbonatee et d'un mortier: etudes experimentales

    Energy Technology Data Exchange (ETDEWEB)

    Lion, M


    The main objective of this study is to evaluate the temperature effect on the hydraulic and poro-mechanical behaviour of a limestone. Many experimental tests (porosity and permeability measurements, uniaxial and hydrostatic compressions tests) were carried out in order to study the thermal treatments effect and so the thermal microcracking effect on rock behaviour. Moreover, an experimental device for permeability measurements under high temperatures (until 200 C) was realized. This experimental device permitted to study the permeability variation of the limestone under thermal stresses. Finally, the behaviour of cementitious materials was studied; the temperature effect on the permeability of a mortar was examined. (author)

  19. Numerical modeling of damage and permeability interaction in rocks. Application for the radioactive waste storage in deep galleries; Modelisation numerique du couplage entre l'endommagement et la permeabilite des roches. Application a l'etude des ouvrages de stockage souterrain

    Energy Technology Data Exchange (ETDEWEB)

    Maleki, K


    The relation between damage and permeability in rocks is a very important subject in industrial applications. It is for example the case of cracks around the radioactive waste storage galleries (EDZ) which can rise considerably the permeability and so make a serious problem for the sealing and the safety of these structures. The same phenomena can occur in the surrounding concrete wall of the nuclear power stations and also in the cracking of the oil-bearing rocks reservoirs. The experimental research on this subject, specially in laboratory G.3S-LMS in Ecole Polytechnique made it possible to determine the orders of damage effect on the permeability change. But a numerical modeling of these phenomena for setting a constitutive behavior law was to be done. This is the principal objective of this thesis. In this project, at first the notion of crack damage is defined. This type of damage is modeled by the disc shaped crack distribution in the 3D space. The disc's geometrical characteristics (radius, direction and opening) obey the statistical distribution laws, depending on the type of loading (compression or extension). The upper and lower limits of the characteristics are fixed according to actual observations, carried out on clay-stone (host rock selected for the realization of an underground research laboratory in Bure). In order to modeling the damage in the porous media, the double porosity concept is considered. A method of homogenization is used to simulate the flow through the network of cracks and porosity. It allows to derive the equivalent permeability of the fractured porous media. The study of the correlations between permeability and damage, obtained by this method for various values of fracture density, made it possible to obtain a relation between permeability and crack damage, for classical types of loading as simple extension and simple compression. To generalize this relation in the case of any specified triaxial loading, the crack damage is put in relation with the notion of mechanical damage, resulting from a mechanical model connecting the damage to stress and strain. Finally, the poro-mechanical behavior law with damage is implemented to the finite elements computer program ESAR-LCPC The modeling of an underground gallery proved that damage calculation by this method is feasible and allows to compute the evolution of the permeability and the variation of the flow entering the gallery as a function of this damage. (author)

  20. The natural seismic hazard and induced seismicity of the european HDR (hot dry rock) geothermal energy project at Soultz-sous-Forets (Bas-Rhin, France); Alea sismique naturel et sismicite induite du projet geothermique europeen RCS (roche chaude seche) de Soultz-sous-Forets (Bas-Rhin, France)

    Energy Technology Data Exchange (ETDEWEB)

    Helm, J.A.


    Development of the Soultz-sous-Forets HDR (Hot Dry Rock) geothermal energy project will involve important fluid injections which will induce micro-seismic events. This thesis discusses the natural seismicity of the region and induced seismicity associated with fluid injections. A catalogue of all historical and instrument seismicity of the Soultz-sous-Forets (SSF) region has been compiled. This seismicity does not correspond to movements along the major tectonic features of the region. The area around SSF has been identified as being one where high heat flow corresponds to low seismicity. The largest well documented seismic event in the region which took place in 1952 had an epicentral intensity of VI. All important data pertaining to the series of seismic events which took place in the region from August to October 1952 have been collected and are presented. This work details the installation and operation of a permanent 3 station network of accelerometers and seismometers around the HDR site. Also the installation and operation of a mobile network of vertical seismometers during fluid injections. 167 micro-seismic events were recorded on the surface network, with magnitudes from -0.5 to 1.9. The preferential alignment of the micro-seismic cloud is N160 deg. Individual focal mechanisms of the larger seismic events correspond to an extensional tectonic regime. Stress inversion of P wave polarities indicates that the maximum stress is vertical and the intermediate and minimum stress axes horizontal. The largest of the horizontal stresses is orientated N124 deg and the smallest N34 deg. Induced seismic movement is taking place on pre-existing fractures controlled by the in situ stress seismic movement is taking place on pre-existing tectonic fractures controlled by the in situ stress field, and the largest of the induced events had a magnitude 1.9. This level of seismicity does not pose any environmental hazard to the region around Soultz-sous-Forets. (author) 151 refs.

  1. Erosion of the Alps: use of Rb-Sr isotopic data from molassic sediments to identify the ages of the metamorphism recorded by the eroded rocks; Erosion des Alpes: histoire metamorphique des roches erodees par l`analyse du couple Rb-Sr des sediments molassiques

    Energy Technology Data Exchange (ETDEWEB)

    Henry, P.; Deloule, E. [Centre National de la Recherche Scientifique (CNRS), 54 - Nancy (France). Centre de Recherches Petrographiques et Geochimiques; Michard, A. [Aix-Marseille-3 Univ., 13 - Marseille (France)


    Rb-Sr isotopic data from Oligocene and Miocene peri-alpine molassic sediments allow us to identify the different periods for which the eroded rocks have or have not recorded an alpine metamorphism. The Chattian and the Burdigalian sediments result from the erosion of rocks for which the latest metamorphic event was variscan, while the Stampian, Aquitanian and ``Helvetian`` sediments show evidence for the erosion of rocks which have recorded alpine metamorphic events. The application of this method to old detrital sediments could permit determination of the ages of the tectonic events which occurred in the sediment source regions. (authors). 18 refs., 6 figs.

  2. Sequence analysis and genotyping of genital Chlamydia trachomatis among patients with suspected-Neisseria gonorrhoeae%拟诊为淋病患者中泌尿生殖道沙眼衣原体基因分型及序列分析

    Institute of Scientific and Technical Information of China (English)

    张娟娟; 卢次勇; 冯铁建; 赵广录; 张丽君; 王峰; 洪福昌; 蓝丽娜; 吴肖冰; 陶小华; 张春来


    目的 了解深圳市拟诊为淋病患者中泌尿生殖道沙眼衣原体的合并感染情况及其基因型分布和序列变异特点.方法 采集401例拟诊为淋病患者的泌尿生殖道分泌物样本,应用Roche Amplicor全自动核酸检测系统对样本进行淋球菌和沙眼衣原体双检,提取DNA,应用巢式聚合酶链反应(nested-PGR)扩增沙眼衣原体主要外膜蛋白基因(omp1)中的VS1~VS2片段,并对其进行序列测定,所获得的序列利用Mega4.0软件与标准参考株进行比对,分析确定其基因型及序列变异情况.结果 401例拟诊为淋病患者中淋球菌的感染率为82.3%(330/401),沙眼衣原体的感染率为24.2%(97/401),淋球菌和沙眼衣原体的合并感染率为21.7%(87/401).97份沙眼衣原体阳性样本中获得73份沙眼衣原体基因片段序列,共检出8个基因型,分别为E型(27.4%)、G/Ga型(23.3%)、D/Da型(16.4%)、F型(13.7%)、J型(11.0%)、H型(5.5%)、B和K型(各1.4%).序列分析发现3例(4.1%)菌株发生错义突变,分别为D/Da型、E型、G/Ga型;F型、H型、J型和K型序列虽多见碱基突变,但均为同义突变.结论淋病患者合并感染沙眼衣原体的比例较高,且泌尿生殖道沙眼衣原体的基因型以E、G/Ga、D/Da和F型为主.序列分析可以为泌尿生殖道沙眼衣原体的分子流行病学研究提供依据.%Objective To understand the prevalence rate of genital Chlamydia trachomatis among a population with suspected-Neisseria gonorrhoeae infection,the distribution of Chlamydia trachomatis genotypes,assess changes in omp1 sequences among patients with Neisseria gonorrhoeae and Chlamydia trachomatis coinfections.Methods Four hundred and one swabs were collected.Chlamydia trachomatis and Neisseria gonorrhoeae were detected by Roche Amplicor System.DNA were extracted from those samples and were amplified by nested PCR.PCR products were sequencing and analyzed by software Mega4.0.Results The prevalence of genital Chlamydia

  3. Comparison of Three Nucleic Acid Amplification Assays of Cerebrospinal Fluid for Diagnosis of Cytomegalovirus Encephalitis (United States)

    Bestetti, Arabella; Pierotti, Chiara; Terreni, Mariarosa; Zappa, Alessandra; Vago, Luca; Lazzarin, Adriano; Cinque, Paola


    The diagnostic reliabilities of three cytomegalovirus (CMV) nucleic acid amplification assays of cerebrospinal fluid (CSF) were compared by using CSF samples from human immunodeficiency virus-infected patients with a postmortem histopathological diagnosis of CMV encephalitis (n = 15) or other central nervous system conditions (n = 16). By using a nested PCR assay, the quantitative COBAS AMPLICOR CMV MONITOR PCR, and the NucliSens CMV pp67 nucleic acid sequence-based amplification assay, sensitivities were 93.3, 86.6, and 93.3%, respectively, and specificities were 93.7, 93.7, and 87.5%, respectively. The COBAS AMPLICOR assay revealed significantly higher CMV DNA levels in patients with diffuse ventriculoencephalitis than in patients with focal periventricular lesions. PMID:11230445

  4. Is real-time PCR better than conventional PCR for Mycobacterium tuberculosis complex detection in clinical samples? (United States)

    Tortoli, Enrico; Urbano, Pasquale; Marcelli, Fiorella; Simonetti, Tullia M; Cirillo, Daniela M


    Cobas Amplicor MTB and later Cobas TaqMan MTB were used to test a very large series of consecutive specimens received for tuberculosis diagnosis. Performance parameters were estimated and compared overall and for separate specimen categories. Both systems showed excellent specificity, and that of TaqMan was the higher. The sensitivities were similar but satisfactory only with respiratory specimens and smear-positive samples.

  5. Development of Automated Whistle and Click Classifiers for Odontocete Species in the Northwest Atlantic Ocean and the Waters Surrounding the Hawaiian Islands (United States)


    New York). Baumann-Pickering, S, Wiggins , S., Hildebrand, J.A., Roch, M.A., Schnitzer, H.U. 2010. Discriminating features of echolocation...America 129: 467–475. Soldevilla, M.S., Henderson, E.E., Campbell, G.S., Wiggins , S.M., Hildebrand, J.A., and Roch, M.A. 2008. Classification of

  6. An Interlingual-based Approach to Reference Resolution (United States)


    miilones de francos o el 14 por eiento de las ventas del grupo. Las inversiones en investigaci6n y desarrollo (I+D) fueron de 1.210 millones de francos... inversiones realizadas totalizaron 223 millones de pesetas. English Translation of Text 93-1 Roche Buys Docteur Andreu The Roche Group acquired the

  7. Before You Search that Locker. (United States)

    Dowling-Sendor, Benjamin


    In DesRoches v Caprio, federal district court ruled in favor of Jim DesRoches who had decided not to consent to a search of his backpack for a pair of allegedly stolen sneakers. Judge Robert G. Doumar decided the need to find the stolen sneakers did not outweigh the students' privacy interest and offered guidelines about school searches for stolen…

  8. Complex geometry and real geometry

    Institute of Scientific and Technical Information of China (English)

    YU; Yanlin; ZHU; Lin; FENG; Xiuhong


    There is a well-known way to generalize the Riemann-Roch operator for Kahler manifold to that for Hermitian manifold. In this paper we show a slightly different way to get a generalized Riemann-Roch operator, which is just the Dirac operator. The difference between the two operators is that the latter one enables the so-called Pythagoras equalities.

  9. Discovery of metal line emission from the Red star in IP Peg during outburst maximum

    CERN Document Server

    Harlaftis, E T


    Observations of the eclipsing dwarf nova IP Peg during outburst reveal metal lines in emission, such as Mg II 4481 A. Analysis using Doppler tomography locates emission of helium and metal lines on the inner Roche lobe of the secondary star. Such multi-line Roche-lobe imaging presents a new tool in mapping the red star's ionization structure.

  10. Effects of Sarin on the Operant Behavior of Guinea Pigs (United States)


    chemistry analyzer (Roche Diagnostics, Nutley, NJ). After 10 left lever presses, the schedule converted to a fixed- The analytical procedure was based... chemistry analyzer (Roche Diagnostics, Nutley, NJ). After 10 left lever presses, the schedule converted to a fixed- The analytical procedure was based on

  11. Insulin and Breast Cancer Risk (United States)


    of three standard samples. Serum glucose was determined on a Cobas Mira automated chemistry analyzer (Roche Diagnostic Systems, Indianapolis, IL). The...was determined on a Cobas Mira automated chemistry analyzer (Roche Diagnostic Systems, Indianapolis, IL). The intrabatch coefficient of variation

  12. The Smart/Empire Tipster IR System (United States)


    State Transducer for Extracting Information from Natural-Language Text. In E. Roche andY. Schabes , editor, Finite-State Language Processing, pages 383...Fran- cisco, CA, 1997. Morgan Kaufmann. [ 16] Roche, E. and Schabes , Y., editor. Finite State De- vices for Natural Language Processing. MIT Press

  13. [Genetic characterization of viruses from the antigenic complex Tyuleniy (Flaviviridae, Flavivirus): Tyuleniy virus (TYUV) (ID GenBank KF815939) isolated from ectoparasites of colonial seabirds--Ixodes (Ceratixodes) uriae White, 1852, ticks collected in the high latitudes of Northern Eurasia--and Kama virus (KAMV) isolated from the Ixodes lividus Roch, 1844, collected in the digging colonies of the middle part of Russian plane]. (United States)

    L'vov, D K; Al'khovskiĭ, S V; Shchelkanov, M Iu; Shchetinin, A M; Deriabin, P G; Samokhvalov, E I; Gitel'man, A K; Botikov, A G


    Genetic research into the Tyuleniy virus (TYUV) (ID GenBank KF815939) isolated in high latitudes from the Ixodes uriae White, 1852, ticks collected in the nesting colonies of the Alcidae (Leach, 1820) birds and Kama virus (KAMV) (ID GenBank KF815940) isolated from the I. lividus ticks collected in the nesting bird colonies in the middle part of the Russian Plane was carried out. Full-genome comparative analysis revealed 70% homology between KAMV and TYUV on the nucleotide level and 74% on the amino acid level. Thus, KAMV is a new member of the TYUV complex belonging to the seabird tick-borne virus group (STBVG) of Flavivirus (Flaviviridae). KAMV is a separate virus and forms separate phylogenetic line together with the TYUV, Meaban virus (MEAV), and Saumarez Reef virus (SREV).

  14. 阿德福韦治疗对慢性乙型肝炎患者Th细胞相关细胞因子水平及HBVDNA的影响%The effect of adefovir dipivoxil therapy on T helper cell cytokines and HBV DNA loads in patients with hepatitis B

    Institute of Scientific and Technical Information of China (English)

    马萍; 宋诗铎; 王磊


    Objective To explore the influence of adefovir dipivoxil (ADV) therapy on the cellular immunity by observing the levels of interferon-γ(IFN-γ), interleukin-4 (IL-4) and their relationship with HBV DNA loads in patients with HBeAg-positive chronic hepatitis B at different time before and after treatment.Methods Sera of 30 patients before ADV therapy and 16 weeks ,52 weeks and 132 weeks after ADV therapy respectively were collected in this study.There were 14 complete response cases(group A), 16 incomplete response cases(group B).Sera of 10 healthy people were chosen as control group(group C).Levels of IFN-γ and IL-4 were detected with enzyme-linked immunosorbent assay; HBV DNA loads were detected by ROCHE COBAS AMPLIC)R HBV MONITOR, lower limit was 103 copy/mL.Results The average IFN-γ level in group A was significantly higher than that of group B (P<0.05)and C(P<0.05), there was no significant difference between group B and C(P>0.05);The level of IL-4 of group A decreased after treatment while group B didn't.HBV DNA loads of all groups had no significant correlation with IFN-γ and IL-4 levels before treatment, but dropped obviously at 16th week after treatment; and the increased level of IFN-γ in group A was significantly higher than that of group B(P<0.05); IL-4 levels in group A decreased gradually, but didn't decreased obviously in group B.Conclusion Cellular immune response of patients with hepatitis B was resumed to some extent after ADV treatment.The resumption level was positively related with the decreased level of HBV DNA load.%目的 观察乙型肝炎e抗原(HBeAg)阳性慢性乙型肝炎患者阿德福韦治疗前后不同时相点血清干扰素(IFN)-γ和白细胞介素(IL)-4的水平,以及其与乙型肝炎病毒(HBV)DNA定量的关系,以探讨阿德福韦治疗对机体免疫状态的影响.方法 采集30例阿德福韦治疗前及治疗16周、52周和132周患者的血清,其中完全应答组14例,部分应答组16

  15. Detección temprana y altamente sensible de citomegalovirus en muestras de plasma humano VIH-positivas



    La detección temprana de citomegalovirus humano puede evitar graves consecuencias en la salud de pacientes inmunocomprometidos; por ello, es importante el empleo de métodos de detección sensibles y precisos. En el presente trabajo comparamos la sensibilidad y la especificidad de detección de tres métodos: 1) COBAS AMPLICOR CMV MONITOR¿ Test (CACM), método comercial validado y semiautomatizado que utiliza la reacción en cadena de la polimerasa (PCR) para detectar la expresión del gen temprano ...

  16. Tere tulemast tagasi, uue põlvkonna utopistid! / Veronika Valk

    Index Scriptorium Estoniae

    Valk, Veronika, 1976-


    Francois Roche ja Eva Castro tulevikuarhitektuuri käsitlevatest ettekannetest Tallinna arhitektuuribiennaali raames toimunud sümpoosionil. Maketinäitus "Viini eksiilid. Helisev arhitektuur" Heliose kinos. Eksponeeritakse Viini Die Angewandte arhitektuurikoolis (juht Hani Rashid) õppivate eestlaste loomingut

  17. Book Reviews

    Directory of Open Access Journals (Sweden)

    Bart Bechtel


    Full Text Available Fair Play: The Moral Dilemmas of Spying. By James M. Olson. The Corporate Spy: Industrial Espionage and Counterintelligence in the Multinational Enterprise with Case Studies (Abridged Version by Edward M. Roche.

  18. Vanemuine otsib teed head teatrit hindava vaatajani / Sven Karja ; interv. Margot Visnap

    Index Scriptorium Estoniae

    Karja, Sven, 1968-


    Vanemuise eesseisvast hooajast. 27. sept. Tartu Sadamateatris esietendunud Donald Margulies' draamast "Kogutud teosed", lavastaja Eva Klemets; novembris esietendusvast Billy Roche'i "Vaene loom vihma käes", lavastaja Liisa Smith ja teistest uuslavastustest lühidalt

  19. Tähelepanu keskmes on viiruslik hepatiit / Marika Kusnets

    Index Scriptorium Estoniae

    Kusnets, Marika


    Ülemaailmsel hepatiidipäeval 19. mail korraldasid Eesti Gastroenteroloogide Selts (EGS) ja Roche Eesti OÜ meediakanalite esindajatele ümarlaua, et aidata teadvustada C-hepatiidi ohtu, testimis- ja ravivõimalusi Eestis

  20. EPL-i ümarlaud : kõige olulisem on vähi varajane avastamine / Ille Grün-Ots

    Index Scriptorium Estoniae

    Grün-Ots, Ille, 1960-2016


    Ümarlaual osalesid TÜ kliinikumi hematoloogia-onkoloogiakliiniku professor Hele Everaus, ravimifirma Roche Eesti OÜ tegevjuht Kadri Mägi, ravifirma GlaxoSmithKline Eesti filiaali juhataja Akshay Mody ja PERH-i keemiaravispetsialist Helis Pokker

  1. (see photo 7503249)

    CERN Multimedia


    C.J. Zilverschoon, Peter Standley, Christian Roche, Jacques Blondel, Susan Neboux-Bland, Pierre Marti, Jurgen Knott; Roberto Cappi, Pierre Tetu, Charles Rufer, A. L'Aire, Patrick Lienard, Julienne de Winter

  2. Sequence Read Archive (SRA) (United States)

    U.S. Department of Health & Human Services — The Sequence Read Archive (SRA) stores raw sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome...

  3. Immune-Focused Drug Shows Promise Against Lung Cancer (United States)

    ... chemotherapy group. The study, published Dec. 12 in The Lancet , was funded by F. Hoffmann-La Roche Ltd. ... Northwell Health Cancer Institute, Lake Success, N.Y.; The Lancet , news release, Dec. 12, 2016 HealthDay Copyright (c) ...

  4. 75 FR 34178 - Investigations Regarding Certifications of Eligibility To Apply for Worker Adjustment Assistance (United States)


    ..., Inc. (State/ One-Stop). 74142 World Color (Workers)... Schaumburg, IL..... 05/25/10 05/21/10. 74143 Providence Watch Cranston, RI....... 05/25/10 05/13/10. Hospital (Workers). 74144 Hoffmann La Roche...

  5. 77 FR 42713 - Notice of Intent to Grant an Exclusive License; PadJack, Inc. (United States)


    ... Technology Transfer Program, 9800 Savage Road, Suite 6541, Fort George G. Meade, MD 20755-6541. FOR FURTHER INFORMATION CONTACT: Marian T. Roche, Director, Technology Transfer Program, 9800 Savage Road, Suite...

  6. The role of taxanes in triple-negative breast cancer: literature review [Corrigendum


    Mustacchi G; De Laurentiis M


    Mustacchi G, De Laurentiis MMustacchi G, De Laurentiis M. Drug Des Devel Ther. 2015;9:4303–4318.On page 4315, Disclosure section, "This work was supported by an unconditional grant from Editree editors, Italy, and both authors received a fee. Giorgio Mustacchi receives honoraria from Roche, Celgene, and EISAI. Michelino De Laurentiis receives honoraria from Roche, Celgene, EISAI, Novartis, and AstraZeneca. The authors report no other conflicts of interest in this work.&...

  7. The Darleen Druyun Debacle: Procurement, Power, and Corruption (United States)


    196 The resignation of Boeing’s CEO, Phil Condit, was also, at least partially, due to the events surrounding Ms. Druyun’s hiring. 197 Press...Ralph Crosby .268 The “blame the system/institution” set may point to an e-mail exchange between Secretary Roche and Ms. Druyun that occurred...268 Secretary Roche had worked with Crosby at Northrop Grumman. Id at 3. 66 not have been surprised at the slime…his day of reckoning will

  8. MODULAR ANALYTICS: A New Approach to Automation in the Clinical Laboratory


    Horowitz, Gary L.; Zaman, Zahur; Blanckaert, Norbert J. C.; Chan, Daniel W.; Dubois, Jeffrey A.; Golaz, Olivier; Mensi, Noury; Keller, Franz; Stolz, Herbert; Klingler, Karl; Marocchi, Alessandro; Prencipe, Lorenzo; McLawhon, Ronald W.; Nilsen, Olaug L.; Oellerich, Michael


    MODULAR ANALYTICS (Roche Diagnostics) (MODULAR ANALYTICS, Elecsys and Cobas Integra are trademarks of a member of the Roche Group) represents a new approach to automation for the clinical chemistry laboratory. It consists of a control unit, a core unit with a bidirectional multitrack rack transportation system, and three distinct kinds of analytical modules: an ISE module, a P800 module (44 photometric tests, throughput of up to 800 tests/h), and a D2400 module (16 photometric tests, throughp...

  9. De geologie van den Cimone di Margno en den Monte di Muggio

    NARCIS (Netherlands)

    Buning, W.L.


    Le terrain étudié (fig. 1) est formé de roches métamorphiques pour plus de la moitié; le reste est formé de sédiments variant du Permien au Trias Inférieur, et de roches appartenant à la culmination éruptivocristalline du Cimone di Margno. Le relief du paysage présente en général des pentes douces.

  10. Intramolecular Oxidative O-Demethylation of an Oxoferryl Porphyrin Complexed with a Per-O-methylated β-Cyclodextrin Dimer. (United States)

    Kitagishi, Hiroaki; Kurosawa, Shun; Kano, Koji


    The intramolecular oxidation of ROCH3 to ROCH2 OH, where the latter compound spontaneously decomposed to ROH and HCHO, was observed during the reaction of the supramolecular complex (met-hemoCD3) with cumene hydroperoxide in aqueous solution. Met-hemoCD3 is composed of meso-tetrakis(4-sulfonatophenyl)porphinatoiron(III) (Fe(III) TPPS) and a per-O-methylated β-cyclodextrin dimer having an -OCH2 PyCH2 O- linker (Py=pyridine-3,5-diyl). The O=Fe(IV) TPPS complex was formed by the reaction of met-hemoCD3 with cumene hydroperoxide, and isolated by gel-filtration chromatography. Although the isolated O=Fe(IV) TPPS complex in the cyclodextrin cage was stable in aqueous solution at 25 °C, it was gradually converted to Fe(II) TPPS (t1/2 =7.6 h). This conversion was accompanied by oxidative O-demethylation of an OCH3 group in the cyclodextrin dimer. The results indicated that hydrogen abstraction by O=Fe(IV) TPPS from ROCH3 yields HO-Fe(III) TPPS and ROCH2(.) . This was followed by radical coupling to afford Fe(II) TPPS and ROCH2 OH. The hemiacetal (ROCH2 OH) immediately decomposed to ROH and HCHO. This study revealed the ability of oxoferryl porphyrin to induce two-electron oxidation.

  11. Dried blood spots for the diagnosis and quantitation of HIV-1: stability studies and evaluation of sensitivity and specificity for the diagnosis of infant HIV-1 infection in Thailand. (United States)

    Leelawiwat, W; Young, N L; Chaowanachan, T; Ou, C Y; Culnane, M; Vanprapa, N; Waranawat, N; Wasinrapee, P; Mock, P A; Tappero, J; McNicholl, J M


    Molecular methods for HIV-1 infection using dried blood-spot (DBS) for HIV-1 CRF01_AE subtypes have not been fully optimized. In this study assays for HIV-1 diagnosis or quantitation were evaluated using infant DBS from Thailand. Paired DBS and whole blood samples from 56 HIV-1 CRF01_AE or B'-infected infants were tested for infant diagnosis using modified Amplicor DNA PCR and NucliSens RNA NASBA and an in-house real-time PCR assay. The Amplicor Monitor viral load (VL) assay, with modifications for DBS, was also evaluated. DBS VL were hematocrit corrected. Stability studies were done on DBS stored at -70 degrees C to 37 degrees C for up to 1 year. The DBS diagnostic assays were 96-100% sensitive and 100% specific for HIV-1 diagnosis. DBS HIV-1 VL were highly correlated with plasma VL when corrected using the actual or an assumed hematocrit factor (r(c)=0.88 or 0.93, respectively). HIV-1 DNA in DBS appeared to be more stable than RNA and could be detected after up to 9 months at most temperatures. DBS VL could be consistently determined when stored frozen. These results show that DBS can be used accurately instead of whole blood for the diagnosis of HIV-1 infection and VL quantitation, particularly if samples are appropriately stored.

  12. A clinical, epidemological, laboratorial, histological and ultrasonographical evaluation of anti-HCV EIA-2 positive blood donors Avaliação clínica, epidemiológica, laboratorial, histológica e ultrassonográfica de doadores de sangue anti-HCV EIA-2 positivos

    Directory of Open Access Journals (Sweden)

    Fernando L. GONÇALES JR


    RIBA-2 positive subjects, in 37.5% of the indeterminate RIBA-2 donors and in 9% of the negative RIBA-2 donors. Chronic hepatitis has also been observed in 50% of the histopathological exams of the anti-HCV EIA-2 reagent donors which were indeterminate RIBA-2. Among 18 blood donors with minimal changes histopathological exam 11 (61% were HCV-RNA positive. Our blood donors anti-HCV reagent generally had clinical, laboratorial and histopathological features observed in patients with chronic HCV hepatitis and a high proportion could be identified in interviews and medical evaluation realized in blood blanks. Generally, these HCV infected donors are identified and discharged only by the serological tests results.Entre 1992 e 1997 foram avaliados, ambulatorialmente, 790 doadores de sangue com teste anti-HCV EIA-2 fortemente reagente (relação entre a densidade ótica da amostra / "cut-off" > 3, que haviam sido detectados na triagem sorológica do banco de sangue. Todos eram negativos para doença de Chagas, sífilis, hepatite B (HBsAg e AIDS. Amostras de sangue foram coletadas, na primeira consulta ambulatorial, para a realização de hemograma, exames bioquímicos e novos testes sorológicos para a HVC (anti-HCV EIA-2. Em 226 doadores anti-HCV EIA-2 repetidamente reagentes, realizou-se o teste suplementar de "immunoblot" para a HVC (RIBA-2. Em 209 doadores, pesquisou-se a presença do RNA do VHC pelo teste do PCR, através de exame automatizado (HCV-AMPLICOR, ROCHE. A ultra-sonografia abdominal foi realizada em 366 doadores e a biópsia hepática em 269 concordantes. Notou-se que 95,6% eram EIA-2 repetidamente reagentes, 94% eram assintomáticos e que apenas 2% referiram icterícia pregressa. Em 47% detectou-se, pelo menos, um fator de risco para a transmissão do VHC, sendo o uso de drogas E.V. o principal deles (27,8%. A transfusão de sangue foi o segundo fator na transmissão da HVC (27,2%. Hepatomegalia foi encontrada em 54%. Esplenomegalia e sinais de hipertens

  13. Systematic Review of the Performance of HIV Viral Load Technologies on Plasma Samples (United States)

    Sollis, Kimberly A.; Smit, Pieter W.; Fiscus, Susan; Ford, Nathan; Vitoria, Marco; Essajee, Shaffiq; Barnett, David; Cheng, Ben; Crowe, Suzanne M.; Denny, Thomas; Landay, Alan; Stevens, Wendy; Habiyambere, Vincent; Perrins, Jos; Peeling, Rosanna W.


    Background Viral load (VL) monitoring is the standard of care in developing country settings for detecting HIV treatment failure. Since 2010 the World Health Organization has recommended a phase-in approach to VL monitoring in resource-limited settings. We conducted a systematic review of the accuracy and precision of HIV VL technologies for treatment monitoring. Methods and Findings A search of Medline and Embase was conducted for studies evaluating the accuracy or reproducibility of commercially available HIV VL assays. 37 studies were included for review including evaluations of the Amplicor Monitor HIV-1 v1.5 (n = 25), Cobas TaqMan v2.0 (n = 11), Abbott RealTime HIV-1 (n = 23), Versant HIV-1 RNA bDNA 3.0 (n = 15), Versant HIV-1 RNA kPCR 1.0 (n = 2), ExaVir Load v3 (n = 2), and NucliSens EasyQ v2.0 (n = 1). All currently available HIV VL assays are of sufficient sensitivity to detect plasma virus levels at a lower detection limit of 1,000 copies/mL. Bias data comparing the Abbott RealTime HIV-1, TaqMan v2.0 to the Amplicor Monitor v1.5 showed a tendency of the Abbott RealTime HIV-1 to under-estimate results while the TaqMan v2.0 overestimated VL counts. Compared to the Amplicor Monitor v1.5, 2–26% and 9–70% of results from the Versant bDNA 3.0 and Abbott RealTime HIV-1 differed by greater than 0.5log10. The average intra and inter-assay variation of the Abbott RealTime HIV-1 were 2.95% (range 2.0–5.1%) and 5.44% (range 1.17–30.00%) across the range of VL counts (2log10–7log10). Conclusions This review found that all currently available HIV VL assays are of sufficient sensitivity to detect plasma VL of 1,000 copies/mL as a threshold to initiate investigations of treatment adherence or possible treatment failure. Sources of variability between VL assays include differences in technology platform, plasma input volume, and ability to detect HIV-1 subtypes. Monitoring of individual patients should be performed on the same

  14. Systematic review of the performance of HIV viral load technologies on plasma samples.

    Directory of Open Access Journals (Sweden)

    Kimberly A Sollis

    Full Text Available BACKGROUND: Viral load (VL monitoring is the standard of care in developing country settings for detecting HIV treatment failure. Since 2010 the World Health Organization has recommended a phase-in approach to VL monitoring in resource-limited settings. We conducted a systematic review of the accuracy and precision of HIV VL technologies for treatment monitoring. METHODS AND FINDINGS: A search of Medline and Embase was conducted for studies evaluating the accuracy or reproducibility of commercially available HIV VL assays. 37 studies were included for review including evaluations of the Amplicor Monitor HIV-1 v1.5 (n = 25, Cobas TaqMan v2.0 (n = 11, Abbott RealTime HIV-1 (n = 23, Versant HIV-1 RNA bDNA 3.0 (n = 15, Versant HIV-1 RNA kPCR 1.0 (n = 2, ExaVir Load v3 (n = 2, and NucliSens EasyQ v2.0 (n = 1. All currently available HIV VL assays are of sufficient sensitivity to detect plasma virus levels at a lower detection limit of 1,000 copies/mL. Bias data comparing the Abbott RealTime HIV-1, TaqMan v2.0 to the Amplicor Monitor v1.5 showed a tendency of the Abbott RealTime HIV-1 to under-estimate results while the TaqMan v2.0 overestimated VL counts. Compared to the Amplicor Monitor v1.5, 2-26% and 9-70% of results from the Versant bDNA 3.0 and Abbott RealTime HIV-1 differed by greater than 0.5log10. The average intra and inter-assay variation of the Abbott RealTime HIV-1 were 2.95% (range 2.0-5.1% and 5.44% (range 1.17-30.00% across the range of VL counts (2log10-7log10. CONCLUSIONS: This review found that all currently available HIV VL assays are of sufficient sensitivity to detect plasma VL of 1,000 copies/mL as a threshold to initiate investigations of treatment adherence or possible treatment failure. Sources of variability between VL assays include differences in technology platform, plasma input volume, and ability to detect HIV-1 subtypes. Monitoring of individual patients should be performed on the same

  15. Tidal disruption of satellites and formation of narrow rings

    CERN Document Server

    Leinhardt, Zoe M; Latter, Henrik N; Kokubo, Eiichiro


    In this paper we investigate the formation of narrow planetary rings such as those found around Uranus and Saturn through the tidal disruption of a weak, gravitationally bound satellite that migrates within its Roche limit. Using $N$-body simulations, we study the behaviour of rubble piles placed on circular orbits at different distances from a central planet. We consider both homogeneous satellites and differentiated bodies containing a denser core. We show that the Roche limit for a rubble pile is closer to the planet than for a fluid body of the same mean density. The Roche limit for a differentiated body is also closer to the planet than for a homogeneous satellite of the same mean density. Within its Roche limit, a homogeneous satellite totally disrupts and forms a narrow ring. The initial stages of the disruption are similar to the evolution of a viscous fluid ellipsoid, which can be computed semi-analytically. On the other hand, when a differentiated satellite is just within the Roche limit only the ma...

  16. Chemically Tunable Transport Phenomena of Functionalized Graphene (United States)

    Leconte, Nicolas; Lherbier, Aurélien; Varchon, Francois; Charlier, Jean-Christophe; Palacios, Juan Jose; Soriano, David; Ordejon, Pablo; Roche, Stephan


    We present an ab initio multiscale study and quantum transport simulations using the Kubo formalism [1] of chemically modified graphene based materials, whose properties are tuned by changing the density and nature of grafted molecular units. Depending on the nature of the introduced molecular bonding different conduction mechanism are obtained, including transition from weak to strong Anderson localization [2,3], as well as spin-dependent phenomena [4] and magnetoresistive fingerprints [5]. [4pt] References: [1] H. Ishii, F. Triozon, N. Kobayashi, K. Hirose, and S. Roche, C. R. Physique 10, 283 (2009) [2] N. Leconte, J. Moser, P. Ordejon, H. Tao, A. Lherbier, A. Bachtold, F. Alsina, C.M. Sotomayor Torres, J.-C. Charlier, and S. Roche, ACS Nano 4, 7, 4033-4038 (2010) [3] N. Leconte, A. Lherbier, F. Varchon, P. Ordejon, S. Roche, and J.-C. Charlier (accepted in PRB) [4] N. Leconte, D. Soriano, S. Roche, P. Ordejon, J.-C. Charlier, and J.J. Palacios, ACS Nano 5, 5, 3987-3992 (2011) [5] D. Soriano, N. Leconte, P. Ordejon, J.-C. Charlier, J.J. Palacios, and S. Roche, Phys. Rev. Lett. 107, 016602 (2011)

  17. Tidal disruption of inviscid protoplanets (United States)

    Boss, Alan P.; Cameron, A. G. W.; Benz, W.


    Roche showed that equilibrium is impossible for a small fluid body synchronously orbiting a primary within a critical radius now termed the Roche limit. Tidal disruption of orbitally unbound bodies is a potentially important process for planetary formation through collisional accumulation, because the area of the Roche limit is considerably larger then the physical cross section of a protoplanet. Several previous studies were made of dynamical tidal disruption and different models of disruption were proposed. Because of the limitation of these analytical models, we have used a smoothed particle hydrodynamics (SPH) code to model the tidal disruption process. The code is basically the same as the one used to model giant impacts; we simply choose impact parameters large enough to avoid collisions. The primary and secondary both have iron cores and silicate mantles, and are initially isothermal at a molten temperature. The conclusions based on the analytical and numerical models are summarized.

  18. Development of a TaqMan assay for the six major genotypes of hepatitis C virus: Comparison with commercial assays

    DEFF Research Database (Denmark)

    Engle, Ronald E; Russell, Rodney S; Purcell, Robert H;


    A quantitative real-time PCR assay was developed that detects genomic RNA from reference strains representing the six major genotypes of hepatitis C virus (HCV) with equal sensitivity and accurately measured HCV RNA in JFH1 HCV-infected Huh7.5 cells. The method is indirectly calibrated to the first...... (Versant HCV RNA 3.0 b-DNA and Amplicor HCV Monitor), that also employ the WHO standard, allowed validation of the TaqMan assay against all major HCV genotypes. Both commercial methods detected HCV RNA over a wide dynamic range, but showed a consistent difference of about 0.3 log10 when evaluating samples...... of different HCV genotypes. The genome titers obtained with the three methods correlated with the infectivity titers previously determined for the HCV reference strains. TaqMan assays have become an essential tool to follow viral load in clinical samples and cell culture-based experiments and this technology...

  19. Comparison of DNA extraction protocols for Mycobacterium Tuberculosis in diagnosis of tuberculous meningitis by real-time polymerase chain reaction

    Directory of Open Access Journals (Sweden)

    Rajeev Thakur


    Full Text Available Background: Several nucleic acid amplification techniques are available for detection of Mycobacterium tuberculosis (MTB in pulmonary and extrapulmonary samples, but insufficient data are available on the diagnostic utility of these techniques in tubercular meningitis where bacilli load is less. The success of final amplification and detection of nucleic acid depends on successful extraction of DNA from the organism. Aims: We performed this study to compare four methods of extraction of MTB DNA from cerebrospinal fluid (CSF samples so as to select one method of DNA extraction for amplification of nucleic acid from clinical samples. Materials and Methods: Four methods of extracting MTB DNA from CSF samples for testing by real-time polymerase chain reaction (PCR were compared: QIAGEN R protocol for DNA purification using QIAamp spin procedure (manual, AMPLICOR R respiratory specimen preparation kit, MagNA Pure R kit extraction, combined manual DNA extraction with automated extraction by MagNA Pure R . Real-time PCR was performed on COBAS TaqMan 48 Analyzer R with known positive and negative controls. Results: The detection limit for the combined manual and MagNA Pure R extraction protocol was found to be 100 copies of MTB DNA per reaction as against 1,000 copies of MTB DNA per reaction by the QIAGEN R , AMPLICOR R , and the MagNA Pure R extraction protocol. Conclusion: The real-time PCR assay employing the combination of manual extraction steps with MagNA Pure R extraction protocol for extraction of MTB DNA proved to be better than other extraction methods in analytical sensitivity, but could not detect less than 10 2 bacilli /ml.

  20. Viral blips during long-term treatment with standard or double dose lamivudine in HBe antigen negative chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)


    AIM: To evaluate safety and effect on hepatitis B virus (HBV) suppression of a long-term treatment with lamivudine (LAM) at standard (100 mg/d) or double (200 mg/d) dose in chronic hepatitis B. METHODS: This was a case study with matched controls (1:3) in patients with chronic hepatitis B with anti-Hbe antibodies. RESULTS: Twelve patients received LAM 200 mg/d and 35 LAM 100 mg/d, for a median of 28 mo. A primary response (PR; I.e. Negative HBV-DNA with Amplicor assay) was achieved in 100% of LAM-200 patients and 83% of LAM-100 patients. A virological breakthrough occurred in 16.7 and 24.7%, respectively, of the PR-patients, with the appearance of typical LAM resistance mutations in all but one patient. Viremia blips (I.e. Transient HBV-DNA below 80 IU/Ml in patients who tested negative at Amplicor assay) were detected using a real time polymerase chain reaction (PCR) and occurred in seven out of nine patients with subsequent BT and in four out of 32 patients with end-of-study response (77.7% vs 12.5%; P = 0.001) at chi-square test). At the end of the study, 51.4% of LAM-100 patients and 83.3% of LAM-200 patients had remained stably HBV-DNA negative. Double-dose LAM was well tolerated. CONCLUSION: Long-term treatment of anti-Hbe positive chronic hepatitis B with double dose lamivudine causes a more profound and stable viral suppression as compared to conventional treatment.

  1. Inhibition of Estrogen-Induced Growth of Breast Cancer by Targeting Mitochondria Oxidants (United States)


    mM NaCl, 0.5% deoxycholate, 0.1% Nonidet P - 40 , 0.1% SDS, 50 mM Tris) containing protease and phosphatase inhibitors (Roche, CA). Equal amounts of...19 deoxycholate, 0.1% Nonidet P - 40 , 0.1% SDS, 50 mM Tris) containing protease and phosphatase inhibitors (Roche, CA). Whole cell lysates (WCL...modulators (MnSOD and catalase), were pre-treated with 40 μM ebselen or 1.0 mM NAC and then challenged with 100 pg/ml estrogensMCF-7 cells containing null

  2. Duas novas espécies de Ptychoderes Schoenherr (Coleoptera, Anthribidae, Anthribinae, Ptychoderini Two new species of Ptychoderes Schoenherr (Coleoptera, Anthribidae, Anthribinae, Ptychoderini

    Directory of Open Access Journals (Sweden)

    José Ricardo M. Mermudes


    Full Text Available Duas novas espécies de Ptychoderes Schoenherr, 1823 são descritas e ilustradas: Ptychoderes magnus sp. nov., da Bolívia e Brasil (localidade-tipo: Ouro Preto d'Oeste, Rondônia e P. jekeli sp. nov. do Brasil e Guiana Francesa (localidade-tipo: Roches de Kourou.The following new species of Ptychoderes Schoenherr, 1823 are described and illustrated (type-localities parenthetic: Ptychoderes magnus sp. nov., from Bolivia and Brazil (Rondônia: Ouro Preto d'Oeste and P. jekeli sp. nov., from Brazil and French Guiana (Roches de Kourou.

  3. The role of taxanes in triple-negative breast cancer: literature review [Corrigendum

    Directory of Open Access Journals (Sweden)

    Mustacchi G


    Full Text Available Mustacchi G, De Laurentiis MMustacchi G, De Laurentiis M. Drug Des Devel Ther. 2015;9:4303–4318.On page 4315, Disclosure section, "This work was supported by an unconditional grant from Editree editors, Italy, and both authors received a fee. Giorgio Mustacchi receives honoraria from Roche, Celgene, and EISAI. Michelino De Laurentiis receives honoraria from Roche, Celgene, EISAI, Novartis, and AstraZeneca. The authors report no other conflicts of interest in this work." should have read "The authors report no conflict of interest with this work".Read the original article

  4. Enplanement and All Cargo Activity (United States)



  5. The future of discovery chemistry: quo vadis? Academic to industrial--the maturation of medicinal chemistry to chemical biology. (United States)

    Hoffmann, Torsten; Bishop, Cheryl


    At Roche, we set out to think about the future role of medicinal chemistry in drug discovery in a project involving both Roche internal stakeholders and external experts in drug discovery chemistry. To derive a coherent strategy, selected scientists were asked to take extreme positions and to derive two orthogonal strategic options: chemistry as the traditional mainstream science and chemistry as the central entrepreneurial science. We believe today's role of medicinal chemistry in industry has remained too narrow. To provide the innovation that industry requires, medicinal chemistry must play its part and diversify at pace with our increasing understanding of chemical biology and network pharmacology.

  6. [Leo Sternbach, an inventor of benzodiazepines]. (United States)

    Uchibayashi, Masao


    A biography of Leo Sternbach, an inventor of benzodiazepine tranquillizers, is presented. It consists of (1) a societal desire for lifestyle pills, (2) Leo's birth in 1908 and youth, (3) education, (4) in Vienna, (5) in Zurich, (6) at Hoffmann-La Roche, Basel, (7) to the New World, (8) at Roche, Nutley NJ, (9) invention of the new drugs, (10) revolution of people's lifestyle, and (11) reward, retirement and obituary in 2005. This paper may be the first comprehensive biography of this remarkable chemist written in Japanese.

  7. Disease: H01221 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available the epithelium are the hallmarks of EBMD. Most patients are asymptomatic before ...roche L, Abitbol M, Schorderet DF A subset of patients with epithelial basement membrane corneal dystrophy have mutations in TGFBI/BIGH3. Hum Mutat 27:553-7 (2006) ...

  8. Research in Pulsed Power Plasma Physics (United States)


    constraints will preclude the use of channels with much with a Tesla coil. Nor is uniformity improved by the use of larger wall radii. a 3 kA prepulse. Driving...Oliphant. 12C. Bruno, J. Delvaux. A. Nicolas . and M. Roche, IEEE Trans. Plasma and P. F. Ottinger. App!. Phys. Lett. 45. 1043 (1984).ISci. PS-IS, 686

  9. Probing thermonuclear burning on accreting neutron stars

    NARCIS (Netherlands)

    Keek, L.


    Neutron stars are the most compact stars that can be directly observed, which makes them ideal laboratories to study physics at extreme densities. Neutron stars in low-mass X-ray binaries accrete hydrogen and helium from a lower-mass companion star through Roche lobe overflow. This matter undergoes

  10. Massively parallel pyrosequencing of the mitochondrial genome with the 454 methodology in forensic genetics

    DEFF Research Database (Denmark)

    Mikkelsen, Martin; Frank-Hansen, Rune; Hansen, Anders Johannes;


    RESULTS: of sequencing of whole mitochondrial genome, HV1 and HV2 DNA with the second generation system (SGS) Roche 454 GS Junior were compared with results of Sanger sequencing and SNP typing with SNaPshot single base extension detected with MALDI-TOF and capillary electrophoresis. We investigat...

  11. Gut microbial markers are associated with diabetes onset, regulatory imbalance, and IFN-γ level in NOD mice

    DEFF Research Database (Denmark)

    Krych, Lukasz; Nielsen, Dennis Sandris; Hansen, Axel Kornerup;


    between the early gut microbiota and immune parameters of non-obese diabetic (NOD) mice in order to select alleged bacterial markers of T1D. Gut microbial composition in feces was analyzed with 454/FLX Titanium (Roche) pyro-sequencing and correlated with diabetes onset age and immune cell populations...

  12. Characterization of Marine Mammal Recordings from the Hawaii Range Complex (United States)


    Germany. 100 pp. Baumann-Pickering, S., S. M. Wiggins, E. Roth , M. A. Roch, H.-U. Schnitzler, and J. A. Hildebrand. (2010). “Echolocation...Institution Woods Hole, MA Ian Boyd University of St. Andrews St. Andrews, Scotland , UK 1 1 1 1 1

  13. Trackline and Point Detection Probabilities for Acoustic Surveys of Cuvier’s and Blainville’s Beaked Whales (United States)


    La Jolla, California 92037 Peter L. Tyack and Mark P. Johnson Scottish Oceans Institute, East Sands, St. Andrews, Fife, Scotland KY15 5NT, United...survey,” Marine Mammal Sci. 21, 429–445. Baumann-Pickering, S., Wiggins, S. M., Roth , E. H., Roch, M. A., Schnitzler, H.-U., and Hildebrand, J. A

  14. Analysis of Proton Transport Experiments. (United States)


    AGENCY NAME & ADDRESS(if different from Controlling Office) IS. SECURITY CLASS. (of this report) Sandia Laboratories Albuquerque, New Mexico 87115...B. 14 21120 Is-sur-Tille France Attn: C. Patou 1 copy C. Peugnet 1 copy M. Roche 1 copy N. Camarcat 1 copy C. Bruno 1 copy J. Barbaro 1 copy Ecole

  15. Molecular Basis for Mycophenolic Acid Biosynthesis in Penicillium brevicompactum

    DEFF Research Database (Denmark)

    Regueira, Torsten Ulrik Bak; Kildegaard, Kanchana Rueksomtawin; Hansen, Bjarne Gram;


    Mycophenolic acid (MPA) is the active ingredient in the increasingly important immunosuppressive pharmaceuticals CellCept (Roche) and Myfortic (Novartis). Despite the long history of MPA, the molecular basis for its biosynthesis has remained enigmatic. Here we report the discovery of a polyketide...

  16. Formal synthesis of (+)-discodermolide. (United States)

    Francavilla, Charles; Chen, Weichun; Kinder, Frederick R


    [structure: see text] Herein we report the formal total synthesis of (+)-discodermolide in 21 steps (longest linear sequence) from commercially available Roche ester. This synthesis features the assembly of C(9-18) and C(19-24) fragments via a metal-chelated aldol coupling reaction.

  17. Development of an oral dosage form of capecitabine with modified release characteristics

    NARCIS (Netherlands)

    Meulenaar, J


    Capecitabine is an orally-administered chemotherapeutic agent used in the treatment of a.o. metastatic breast, gastric and colorectal cancer and is commercially available as an immediate release tablet (Xeloda®, Roche). Capecitabine is a pre-pro-drug that is enzymatically converted into 5-fluorourac

  18. Two new species of Metrosideros (Myrtaceae) from New Caledonia: dual characterisation with morphology and nrDNA sequence variation

    NARCIS (Netherlands)

    Dawson, John W.; Whitaker, Anthony H.; Whitaker, Vivienne A.; Gardner, Richard C.; Wright, Shane D.


    We have identified two new species of Metrosideros subg. Mearnsia (Myrtaceae) growing on, and apparently endemic to, the massif known as the Roches d’Ouaïème in New Caledonia. Of these two species, we include M. whitakeri in sect. Mearnsia and M. rotundifolia in sect. Calyptropelala. Both morphologi

  19. Validation of a norovirus multiplex real-time RT-PCR assay for the detection of norovirus GI and GII from faeces samples.

    LENUS (Irish Health Repository)

    Jones, S


    Norovirus is a leading cause of infectious non-bacterial gastroenteritis. The virus is highly contagious and has multiple modes of transmission, presenting a growing challenge to hospital-based healthcare. In this study, a total of 120 stool samples are tested for the presence of norovirus GI and GII by the Roche two-step Lightcycler 2.0 assay incorporating primers and probes produced by TIB Molbiol, and the results are compared with results from the National Virus Reference Laboratory. The Roche\\/TIB Molbiol assay produced 51 positive results and 69 negative results. Discrepancy analysis was performed for six conflicting results using a second real-time polymerase chain reaction (PCR) assay (Roche\\/TIB Molbiol) and this confirmed that four of the five discrepant positive results were true positives. A single discrepant negative result generated by the Roche assay remained negative using the second assay. Sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were calculated to be 98%, 98.6%, 98.0% and 98.6%, respectively. Melting curve analysis was used to differentiate genogroups I and II and this showed that 92% of strains belonged to genogroup II.

  20. Evaluation of a radioimmunoassay (/sup 125/I) kit for cannabinoid metabolites in urine and whole blood

    Energy Technology Data Exchange (ETDEWEB)

    Childs, P.S.; McCurdy, H.H.

    The Abuscreen kit (Roche Diagnostics) for the analysis of 9/-tetrahydrocannabinol-9-carboxylic acid and other cannabinoids in urine was evaluated in terms of its accuracy, reproducibility, and sensitivity. A procedure is also presented for the analysis of total cannabinoids in whole blood using the RIA kit.

  1. Theory of the Tetragonal-to-Orthorhombic Structural Phase Transition in La2CuO4. (United States)


    J. G. Bednorz and K. A. Muller, Z. Phys. B j4, 189 (1986). 2. C. W. Chu, P. H. Hor, R. L. Meng, L. Gao, Z. J. Huang and Y. Q. Wang , Phys. Rev. Lett...Chemistry University of2 chester Pennsylvania State University Roches ew York 14627 University Park, Pennsylvania 16802 r. G. Rubloff Dr. Roald

  2. Les Basaltes de l’Atlas de Béni-Mellal (Haut Atlas Central, Maroc : un Volcanisme Transitionnel Intraplaque Associé aux Stades de L’évolution Géodynamique du Domaine Atlasique

    Directory of Open Access Journals (Sweden)

    Jaouad Guezal


    Full Text Available Les roches basaltiques de l’Atlas de Béni-Mellal (Haut-Atlas Central, Maroc, mises en place, dans les différents bassins synclinaux de la région, se manifestent en deux horizons volcaniques B1 et B2. Les deux événements éruptifs sont d’âge Jurassique moyen pour les coulées de l’horizon B1 et d’âge Crétacé inférieur pour celles de l’horizon B2. Les roches sont caractérisées par un faciès des basaltes à olivines. La composition de leurs clinopyroxènes est marquée par deux tendances : augite au niveau des basaltes à olivine du B1 et diopside au niveau de ceux du B2. La composition géochimique sur roche totale dévoile le caractère transitionnel de ces roches. La relative dissimilitude géochimique signalée au niveau des basaltes des deux horizons peut être expliquée par un début du changement de la nature du magmatisme de la région en relation avec l’évolution géodynamique du domaine atlasique.

  3. Investigating the Regulation and Potential Role of Nonhypoxic Hypoxia-Inducible Factor 1 (HIF-1) in Aromatase Inhibitor Resistant Breast Cancer (United States)


    protease and phosphatase inhibitors (Roche) by sonication and incubation for 20 min at 4 C. Lysed samples were centrifuged at 14,000 rpm for 20 min...Dontu G, Mantle ID, Patel S, Ahn NS, Jackson KW, Suri P, Wicha MS (2006) Hedgehog signaling and Bmi-1 regulate self- renewal of normal and malignant

  4. SWIFT J1749.4-2807: A neutron or quark star?

    CERN Document Server

    Yu, Junwei


    We investigate an unique accreting millisecond pulsar with X-ray eclipses, SWIFT J1749.4$-$2807 (hereafter J1749), and try to limit the binary system by various methods including that of the Roche lobe, the mass-radius relations of both a main sequence (MS) and a white dwarf (WD) companion stars, as well as the measured mass function of the pulsar. The calculations are based on the assumption that the radius of the companion star has reached its Roche radius (or at 90\\%), but the pulsar's mass has not been assumed to be a certain value. Our results are as follows. The companion star should be a MS. For the case that the radius equals to its Roche one, we have a companion star with mass $M\\simeq 0.51 M_{\\odot}$ and radius $R_{\\rm c}\\simeq 0.52R_{\\odot}$, and the inclination angle is $i\\simeq 76.5^{\\circ}$; for the case that the radius reaches 90\\% of its Roche one, we have $M\\simeq 0.43M_{\\odot}$, $R_{\\rm c}\\simeq 0.44R_{\\odot}$ and $i\\simeq 75.7^{\\circ}$. We also obtain the mass of J1749, $M_{\\rm p}\\simeq 1M_...

  5. Improving the Cybersecurity of U.S. Air Force Military Systems Throughout Their Life Cycles (United States)


    James D. Powers, Elizabeth Bodine-Baron, Bernard Fox, Lauren Kendrick, Michael H. Powell Report Documentation Page Form ApprovedOMB No. 0704...rather than by an inherently secure design. 7 See also Baldwin , Dahmann, and Goodnight, 2011. 7...of more proximate objectives. A common pitfall of analysis is to adopt as goals the most clearly defined and easily measured phenomena. James Roche

  6. 78 FR 25259 - Notice of Intent To Grant an Exclusive License; Integrata Security, LLC (United States)


    ... filed with the National Security Agency Technology Transfer Program, 9800 Savage Road, Suite 6848, Fort George G. Meade, MD 20755-6848. FOR FURTHER INFORMATION CONTACT: Marian T. Roche, Director, Technology Transfer Program, 9800 Savage Road, Suite 6848, Fort George G. Meade, MD 20755-6848, telephone (443)...

  7. Distinguishing Plasmodium falciparum treatment failures from re-infections by using polymerase chain reaction genotyping in a holoendemic area in northeastern Tanzania

    DEFF Research Database (Denmark)

    Magesa, S M; Mdira, K Y; Farnert, A


    susceptibility to a combination of sulfadoxine/pyrimethamine (Fansidar; F. Hoffmann La Roche, Basel, Switzerland). Blood samples were collected before treatment and on days 7, 14, or 28 post-treatment in 51 asymptomatic children, of which 26 could not clear parasitemia within seven days post-treatment. Among...

  8. Multiple nuclear ortholog next generation sequencing phylogeny of Daucus (United States)

    Next generation sequencing is helping to solve the data insufficiency problem hindering well-resolved dominant gene phylogenies. We used Roche 454 technology to obtain DNA sequences from 93 nuclear orthologs, dispersed throughout all linkage groups of Daucus. Of these 93 orthologs, ten were designed...

  9. [Examples of the keeping of drugs during the XVIth, XVIIth and XVIIIth centuries: silenes and drawers]. (United States)

    Fievet, B


    Silenes are wooden boxes with colourful paintings for the keeping of drugs and can be found nowadays in three collections in France: Hotel-Dieu in Troyes (Aube), a pharmacy in Bauge (Maine-et-Loire) and St-Roch museum in Issoudun (Indre). Drawers can be related to silenes but they are more ordinary and less attractive.

  10. Radiation of accretion discs: the eclipses

    Energy Technology Data Exchange (ETDEWEB)

    Schwarzenberg-Czerny, A.


    Light curves have been calculated for eclipses of the accretion disc in a cataclysmic binary. The Roche geometry of the cool component was taken into account and the stellar atmospheres were interpolated to provide the local spectrum of the radiation from the disc. The dependence of the light curve on the parameters of the disc is discussed.

  11. Spiral Learning: An Introductory Course on Reading LOTE Online (United States)

    Schuetze, Ulf


    In teaching reading, authentic texts with abundant lexical items and complex grammatical structures are typically used (Roche, 1992). Unfortunately, this has been a challenge for students enrolled in reading courses developed for English as a Second Language (ESL) as well as Languages Other Than English (LOTE) students. Students often translate…

  12. The Advantage of Reading over Listening Text Comprehension in Down Syndrome: What Is the Role of Verbal Memory? (United States)

    Roch, Maja; Florit, Elena; Levorato, M. Chiara


    The current study was designed to investigate the role played by verbal memory in the advantage shown by individuals with Down syndrome in reading over listening text comprehension (Roch & Levorato, 2009). Two different aspects of verbal memory were analyzed: processing load and coding modality. Participants were 20 individuals with Down syndrome,…

  13. Kevin Stuart among Mongolian English-learners in Huhhot in the Mid-1980s

    Directory of Open Access Journals (Sweden)

    Mandula Borjigin


    Full Text Available Mandula Borjigin, Narisu Narisu, and Chuluu Ujiyediin. 2015. Kevin Stuart among Mongolian English-learners in Huhhot in the Mid-1980s in Gerald Roche, Keith Dede, Fernanda Pirie, and Benedict Copps (eds Asian Highlands Perspectives 37 Centering the Local, A Festschrift for Dr. Charles Kevin Stuart on the Occasion of his Sixtieth Birthday, 296-298.

  14. Operate A Chemical Surety Program And Studies Supporting The Medical Chemical Defense Research Program (United States)


    Administrations Following Intravenous Poisoning with Potassium Cyanide in Adult Male Beagle Dogs...Administrations Following Intravenous Poisoning with Potassium Cyanide in Adult Male Beagle Dogs Task Leader: Dr. Frances Reid / Dr. Brian Roche...agents, synthesis , pharmacokinetics, efficacy testing, seizure control, bioscavengers, toxicology testing and test kit evaluations. In addition, TO

  15. La arquitectura revisionista y orgánica en Estados Unidos


    González Capitel, Antón


    5. La arquitectura revisionista y orgánica en Estados Unidos 5. l. Desarrollo y revisión de la arquitectura moderna: Philip Johnson y Paul Rudolph 5. 2. Eclecticismo y organicismo en la obra de Eero Saarinen 5. 3. La obra de Kevin Roche

  16. Replacement Solvent Identification for the Laundry and Decontamination Drycleaning System (LADDS) (United States)


    Daikin Industries Chemical producer DuPont-Mitsui Fluorochemicals Chemical producer Ebara Equipment manufacturer Hitachi Ltd. Equipment...out schedules have seriously impacted several manufacturers’ decisions to move ahead with these compounds (Akzo, Allied, Asahi, Central, Daikin ...companies include Akzo Chemicals, Allied-Signal, Asahi Glass, Elf Atochem, Daikin Industries, DuPont, Hoechst Celanese, ICI, LaRoche Chemicals

  17. Effect of T-2 Toxin, Fasting, and 2-Methyl-thiazolidine-4-carboxylate, a Glutathione Prodrug, on Hepatic Glutathione Levels1,2 (United States)


    method of Tietz (1969). This method3 was specifically modified for automated analysis on a Cobas-Bio Automated Chemistry Analyzer (Roche Analytical...automated analysis on a Cobas-Bio Automated Chemistry Analyzer . Enzymatic activity was determined in the linear range for both time and protein. Protein was

  18. High Frequency Acoustic Recording Package Data Summary Report, PS 05, August 4, 2008 - January 6, 2009 (United States)


    frequency bins; middle panel – humpback whale ( Megaptera novaeangliae ) song, sampling frequency 10 kHz, 5 s/10 Hz time/frequency bins; lower panel...Roch San Diego State University San Diego, CA Anne Douglas Cascadia Research Collective Olympia, WA Julie Rivers COMPACFLT Pearl

  19. Trends in underlying causes of death in people with HIV from 1999 to 2011 (D:A:D)

    DEFF Research Database (Denmark)

    Smith, Colette J; Ryom, Lene; Weber, Rainer;


    BACKGROUND: With the advent of effective antiretroviral treatment, the life expectancy for people with HIV is now approaching that seen in the general population. Consequently, the relative importance of other traditionally non-AIDS-related morbidities has increased. We investigated trends over t...... Ingelheim, Bristol-Myers Squibb, Gilead Sciences, ViiV Healthcare, Merck, Pfizer, F Hoffmann-La Roche, and Janssen Pharmaceuticals....

  20. Nonlinear Time Series Analysis via Neural Networks (United States)

    Volná, Eva; Janošek, Michal; Kocian, Václav; Kotyrba, Martin

    This article deals with a time series analysis based on neural networks in order to make an effective forex market [Moore and Roche, J. Int. Econ. 58, 387-411 (2002)] pattern recognition. Our goal is to find and recognize important patterns which repeatedly appear in the market history to adapt our trading system behaviour based on them.

  1. Molecular Recognition of DNA. Synthesis of Novel Bases for Triple Helix Formation (United States)


    Southern Lecturer Cancer Therapy into the 21st Century Symposium: Plenary Lecturer Hoffmann La Roche: Max Hoffer Memorial Lecturer Purdue University: H. C...Within the context of molecular engineering to atomic specifications, we view nucleic acids as a 1st generation "molecular lego set" that is very

  2. Negative Suppressors of Oncogenic Activation of the Met Receptor Tyrosine Kinase (United States)


    We thank members of the Park lab, Dr. Sergio Grinstein and Dr. Stephane Laporte for their helpful comments on the manuscript. We would like to...of Hrs couples receptor sorting to vesicle formation. Journal of cell science 116: 4169-4179 Veracini L, Franco M, Boureux A, Simon V, Roche S

  3. The American cranberry mitochondrial genome reveals the presence of selenocysteine (tRNA-Sec and SECIS) insertion machinery in land plants (United States)

    The American cranberry (Vaccinium macrocarpon Ait.) mitochondrial genome was assembled and reconstructed from whole genome 454 Roche GS-FLX and Illumina shotgun sequences. Compared with other Asterids, the reconstruction of the genome revealed an average size mitochondrion (459,678 nt) with comparat...

  4. 电化学发光法联合检测HIV抗原抗体试剂的评价%The evaluation of Elecsys HIV Combi reagent in detecting HIV antigen and antibody

    Institute of Scientific and Technical Information of China (English)

    周琰; 宋斌斌; 郑晓虹; 郭玮; 潘柏申


    目的 评价Roche电化学发光法联合检测HIV抗原抗体试剂的检测性能.方法 收集包括健康献血员、人类免疫缺陷病毒( HIV)感染者、丙型肝炎病毒(HCV)抗体阳性和高危人群在内的血清样本1 327例.购买美国BBI公司的7套HIV血清转换盘共计51份血清样本.采用Roche cobas e411(架式系统)电化学全自动免疫分析仪和相应的检测试剂、校准品和质控品以及检测参数进行测定.以Roche Elecsys HIV Combi试剂盒为评估试剂,以生物梅里埃Vironostika Uni-FormⅡplus O HIV抗体试剂盒为参比试剂.定性检测血清HIV p24抗原和HIVl/2抗体并评价检测方法分析性能、结果一致性和血清转化灵敏度.结果 Roche Elecsys HIV Combi试剂盒批内和天间不精密度良好,符合临床使用要求.血清样本检测结果和参比试剂检测结果一致性完全符合.评估试剂的血清转化灵敏度比参比试剂平均提前了5.1d.结论 Roche Elecsys HIV Combi试剂盒具有良好的精密度和血清转换灵敏度,有利于筛查HIV早期感染,能为临床HIV早期诊断、随访监测提供有效的实验室诊断依据.%Objective To evaluate the performance of Roche Elecsys HIV Combi reagent in detecting HIV p24antigen and HIV1/2 antibody. Methods A total of 1 327 serum samples were collected for healthy subjects, patients with human immunodeficiency virus (HIV) infection and patients with antibody positive and high-risk hepatitis C virus ( HCV). The 1 327 serum samples were detected by Roche cobas e411 Elecsys HIV Combi reagent and Vironostika UniFormll plus 0 HIV reagent, respectively. The both reagents were used to detect 51 serum samples of 7 seroconversion panels from American BBI company, and the analytical performance, consistency and the sensitivity of seroconversion were evaluated. Roche Elecsys HIV Combi reagent was as evaluation reagent, and Vironostika Uni-Form II plus 0 HIV reagent was as reference reagent. Results The Roche Elecsys

  5. Second-generation sequencing of forensic STRs using the Ion Torrent™ HID STR 10-plex and the Ion PGM™

    DEFF Research Database (Denmark)

    Fordyce, Sarah L; Mogensen, Helle Smidt; Børsting, Claus;


    Second-generation sequencing (SGS) using Roche/454 and Illumina platforms has proved capable of sequencing the majority of the key forensic genetic STR systems. Given that Roche has announced that the 454 platforms will no longer be supported from 2015, focus should now be shifted to competing SGS...... platforms, such as the MiSeq (Illumina) and the Ion Personal Genome Machine (Ion PGM™; Thermo Fisher). There are currently several challenges faced with amplicon-based SGS STR typing in forensic genetics, including current lengths of amplicons for CE-typing and lack of uniform data analysis between......) analysis of sensitivity; (3) typing of mixtures; and (4) typing of biological crime case samples. Full profiles and concordant results between replicate SGS runs and CE-typing were observed for all control samples. Full profiles were seen with DNA input down to 50pg, with the exception of a single locus...

  6. A Code for Stellar Binary Evolution and its Application to the Formation of Helium White Dwarfs

    CERN Document Server

    Benvenuto, O G


    We present a numerical code intended for calculating stellar evolution in close binary systems. In doing so, we consider that mass transfer episodes occur when the stellar size overflows the corresponding Roche lobe. In such situation we equate the radius of the star with the equivalent radius of the Roche lobe. This equation is handled implicitly together with those corresponding to the whole structure of the star. We describe in detail the necessary modifications to the standard Henyey technique for treating the mass loss rate implicitly together with thin outer layers integrations. We have applied this code to the calculation of the formation of low mass, helium white dwarfs in low mass close binary systems. We found that the global numerical convergence properties are fairly good. In particular, the onset and end of mass transfer episodes is computed automatically.

  7. NP Pavonis : Differential Corrections Analysis of the UBV Observations

    Directory of Open Access Journals (Sweden)

    M. A. Cerruti


    Full Text Available Se presenta el primer análisis fotoeléctrico de la binaria NP Pav basado en curvas de luz U, B y V y modelado usando el tratamiento de Wilson & Devinney. Las curvas están definidas por 3861 observaciones individuales. Las componentes y la orbita parecen, en general, ser estables. La primaria, más masiva y más luminosa, es la componente de mayor tamaño y está eclipsada en el mínimo primario y no llena su lóbulo de Roche (94%. La secundaria prácticamente llena su lóbulo de Roche (99% y tiene una diferencia de temperatura de =1330oK. Los elementos absolutos se obtuvieron suponiendo que la primaria sigue la relación masa-luminosidad.

  8. NP Pavonis : Differential Corrections Analysis of the UBV Observations


    M. A. Cerruti


    Se presenta el primer análisis fotoeléctrico de la binaria NP Pav basado en curvas de luz U, B y V y modelado usando el tratamiento de Wilson & Devinney. Las curvas están definidas por 3861 observaciones individuales. Las componentes y la orbita parecen, en general, ser estables. La primaria, más masiva y más luminosa, es la componente de mayor tamaño y está eclipsada en el mínimo primario y no llena su lóbulo de Roche (94%). La secundaria prácticamente llena su lóbulo de Roche...

  9. Biosimilars lining up to compete with Herceptin--opportunity knocks. (United States)

    Nelson, Kevin M; Gallagher, Patrick C


    Trastuzumab is a monoclonal antibody developed by Genentech as a treatment for breast cancer and gastric cancer when the cancer cells overexpress HER2, a membrane-bound receptor activated by epidermal growth factor. Now marketed by Roche under the trade name Herceptin, trastuzumab has been readily adopted as treatment for some of the most invasive types of breast cancer. The cost for Herceptin is over $50,000 for a full course of treatment. With the development of regulatory pathways for biosimilar products, and the imminent expiry of patents covering Herceptin, several companies have developed biosimilar trastuzumab products. As biosimilar manufacturers look for opportunities to market biosimilar trastuzumab products, Roche has positioned itself to protect its market by developing additional anti-HER2 products complementary to Herceptin. The advent of competition from biosimilars should bring some opportunity for cost savings for patients, as well as incentive for continued advancement in development of better treatments to fight breast cancer.

  10. Tidal Forces in Naked Singularity Backgrounds

    CERN Document Server

    Goel, Akash; Roy, Pratim; Sarkar, Tapobrata


    The end stage of a gravitational collapse process can generically result in a black hole or a naked singularity. Here we undertake a comparative analysis of the nature of tidal forces in these backgrounds. The effect of such forces is generically exemplified by the Roche limit, which predicts the distance within which a celestial object disintegrates due to the tidal effects of a second more massive object. In this paper, using Fermi normal coordinates, we numerically compute the Roche limit for a class of non-rotating naked singularity backgrounds, and compare them with known results for Schwarzschild black holes. Our analysis indicates that there might be substantially large deviations in the magnitudes of tidal forces in naked singularity backgrounds, compared to the black hole cases. If observationally established, these can prove to be an effective indicator of the nature of the singularity at a galactic centre.

  11. Introduction to the application of QbD principles for the development of monoclonal antibodies. (United States)

    Finkler, Christof; Krummen, Lynne


    Quality by Design (QbD) is a global regulatory initiative with the goal of enhancing pharmaceutical development through the proactive design of pharmaceutical manufacturing process and controls to consistently deliver the intended performance of the product. The principles of pharmaceutical development relevant to QbD are described in the ICH guidance documents (ICHQ8-11). An integrated set of risk assessments and their related elements developed at Roche/Genentech were designed to provide an overview of product and process knowledge for the production of a recombinant monoclonal antibody. This chapter introduces a publication series on the application of Quality by Design for biopharmaceuticals, with a focus on the development of recombinant monoclonal antibodies. The development of and overview on the QbD concept applied by Roche and Genentech is described and essential QbD elements are presented.

  12. Meeting of Directors and Heads of Department from CEA-Saclay, France

    CERN Multimedia

    Patrice Loïez


    Directors and Heads of Department from the Saclay centre of the French atomic energy commission (CEA) visited CERN in December. Here, on a tour of CERN's LHC magnet test facility, LHC division leader Philippe Lebrun (right) explains to visitors the myriad connections of an LHC superconducting dipole magnet. Photo 01: Left to right: J.Y. Gascoin, J. Feltesse, J.-P. Pervès, and Ph. Lebrun. Photo 02: Left to right: L. Laurent, B. Mercier, M. Lieven, B. Mansoulié, M. Pallier, M. Roche, G. Carola, J.Y. Gascoin, A. Masurel, X. Vitart, F. Kircher, J. Feltesse, J.-P. Pervès, and H. Barbelin. Photo 03: Left to right: Ph. Pallier, M. Roche, P. Laurent, J.-Y. Gascoin, G. Carola, A. Masurel, J. Feltesse, J.-P. Pervès, H. Barbelin, and Ph. Lebrun.

  13. High-throughput sequencing offers insight into mechanisms of resource partitioning in cryptic bat species

    DEFF Research Database (Denmark)

    Razgour, Orly; Clare, Elizabeth L.; Zeale, Matt R.K.


    Sympatric cryptic species, characterized by low morphological differentiation, pose a challenge to understanding the role of interspecific competition in structuring ecological communities. We used traditional (morphological) and novel molecular methods of diet analysis to study the diet of two...... cryptic bat species that are sympatric in southern England (Plecotus austriacus and P. auritus) (Fig. 1). Using Roche FLX 454 (Roche, Basel, CH) high-throughput sequencing (HTS) and uniquely tagged generic arthropod primers, we identified 142 prey Molecular Operational Taxonomic Units (MOTUs) in the diet...... of the cryptic bats, 60% of which were assigned to a likely species or genus. The findings from the molecular study supported the results of microscopic analyses in showing that the diets of both species were dominated by lepidopterans. However, HTS provided a sufficiently high resolution of prey identification...

  14. Le grès ferrugineux de Puisaye : provenance et utilisation architecturale

    Directory of Open Access Journals (Sweden)

    Lucile Subtil


    Full Text Available L’objectif de cette étude était de définir le faciès du grès ferrugineux de Puisaye ainsi que ses zones d’exploitation, puis de déterminer l’utilisation architecturale de cette roche dans les édifices monumentaux (XIe au XVIe siècle et d’en définir les aires de diffusion.Cette roche est issue d’intercalations gréseuses et locales à l’intérieur de l’importante formation détritique de l’Albien (Crétacé inférieur. Cet ensemble forme une écharpe, d’orientation sud-ouest - nord-est entre l’Yonne...

  15. A PIONIER and incisive look at the interacting binary SS Lep

    CERN Document Server

    Blind, N; Berger, J -P; Lebouquin, J -B; Mérand, A


    Symbiotic stars are eccellent laboratories to study a broad range of poorly understood physical processes, such as mass loss of red giants, accretion onto compact objects, and evolution of nova-like outbursts. As their evolution is strongly influenced by the mass transfer episodes, understanding the history of these systems requires foremost to determine which process is at play: Roche lobe overflow, stellar wind accretion, or some more complex mixture of both. We report here an interferometric study of the symbiotic system SS Leporis, performed with the unique PIONIER instrument. By determining the binary orbit and revisiting the parameters of the two stars, we show that the giant does not fill its Roche lobe, and that the mass transfer most likely occurs via the accretion of an important part of the giant's wind.

  16. Further considerations on contracting solar nebula (United States)

    Rawal, J. J.


    Kepler's third law of the planetary system is analyzed in terms of Prentice's (1978a) modern Laplacian theory and Rawal's (1984) Roche limit concept. Prentice has determined, based on laws of conservation of mass and angular momentum and the concept of supersonic turbulent convection, that the ratio of orbital radii of successively disposed gaseous rings is a constant of approximately 1.69. Rawal studied the relationship between the supersonic turbulent convection concept and Roche limit and established a limit of 1.442. It is concluded that Kepler's law determines the rotation period of the sun at the time of its formation at the present radius to be equal to 0.1216 d. The correlation between orbital periods of the radii and resonant structure is investigated.

  17. Contraction of the solar nebula (United States)

    Rawal, J. J.


    The concept of Roche limit is applied to the Laplacian theory of the origin of the solar system to study the contraction of a spherical gas cloud (solar nebula). In the process of contraction of the solar nebula, it is assumed that the phenomenon of supersonic turbulent convection described by Prentice (1978) is operative. It is found that the radius of the contracting solar nebula follows Titius-Bode law Rp = R_sun; ap, where R_sun; is the radius of the present Sun and a = 1.442. The consequences of the relation are also discussed. The aim, here, is an attempt to explain, on the basis of the concept of Roche limit, the distribution of planets in the solar system and try to understand the physics underlying it.

  18. The Role of BRCA1 Domains and Motifs in Tumor Suppression (United States)


    media was supplemented with penicillin and streptomycin. Transfections were performed using Fugene 6 (Roche) according to the manufacturer’s... hypersensitive to ionizing radiation (IR) and develop *Correspondence to: Alvaro N.A. Monteiro; H. Lee Moffitt Cancer Center; 12902 Magnolia Drive; Tampa, supplemented with 7.5% FBS, 100 units/mL penicillin /streptomycin and 1.25 μg/ mL amphotericin B (Sigma, St. Louis MO). HCT116 wild-type and Chk2

  19. Strategy, Requirements, and Forces The Rising Imperative of Air and Space Power (United States)


    Operations,” Inside the Air Force, Nov. 1, 2002. 195 Donald Rumsfeld and Paul Wolfowitz , Pentagon news briefing, May 8, 2002. 196James G. Roche, Defense...Air Force Magazine, February 2002. 208 Lt. Gen. Charles Wald , USAF Deputy Chief of Staff for Air & Space Operations, interview with John Tirpak...2002. _______ and Deputy Secretary of Defense Paul Wolfowitz , Pentagon news briefing, May 8, 2002. RYAN, Gen. Michael E., Statement to the Senate Armed

  20. Biochemical Characterisation of TSC1 and TSC2 Variants Identified in Patients with Tuberous Sclerosis Complex (United States)


    0.5% Nonidet P - 40 , 1 mmol/L phenylmethanesulfonyl fluoride, protease inhibitor cocktail; Roche, Indianapolis, IN) and incubated for 1 hour at 4-C...Coevoets et al., 2009 pathogenic pathogenic 40 c.5238_5255del ( p .1746del6/ 1723del6) - LOVD pathogenic unstable, destabilising, pathogenic 41...M, Maat-Kievit A, van Essen T, van Spaendonck-Zwarts, Jansen F, Helderman P , Bartalini G, Vierimaa O, Penttinen M, van den Ende J, van den Ouweland

  1. Toxicity Evaluation of Engineered Nanomaterials (Phase 1 Studies) (United States)


    EDTA, 1% Nonidet P - 40 ) supplemented with Complete Mini Protease Inhibitor Cocktail (Roche Diagnostics, Indianapolis, IN) and frozen at -80°C for at...1996, 59, 61–83. [ 40 ] Providence, K.; Higgins, S.; Mullen, A.; Battista, A.; Samarakoon, R.; Higgins, C.; Wilkins- Port, C.; Higgins, P . SERPINE1...94 Figure 40 . Comparison images of CuO NP 40nm at 6 months. .................................. 94 Figure 41. Comparison images of Cu MSP

  2. Studies of an x ray selected sample of cataclysmic variables. Ph.D. Thesis (United States)

    Silber, Andrew D.


    Just prior to the thesis research, an all-sky survey in hard x rays with the HEAO-1 satellite and further observations in the optical resulted in a catalog of about 700 x-ray sources with known optical counterparts. This sample includes 43 cataclysmic variables, which are binaries consisting of a detached white-dwarf and a Roche lobe filling companion star. This thesis consists of studies of the x-ray selected sample of catalcysmic variables.

  3. 17-DMAG Diminishes Hemorrhage-Induced Small Intestine Injury by Elevating Bcl-2 Protein and Inhibiting iNOS Pathway, TNF-alpha Increase, and Caspase-3 Activation (United States)


    Hemorrhagic shock has been shown to cause systemic inflammation response syndrome (SIRS), multiple organ dysfunction syndrome (MODS), and multiple organ...vehicle) was administered at various doses to mice orally using feeding needles 16 h prior to hemorrhage. Histopathologic assessment. Jejunal...Bioluminescence Assay Kit HS II (Roche, Mannheim, Germany). Luminescence was measured with TD-20/20 luminometer 16 ( Turner Designs, Sunnyvale, CA, USA

  4. Equivalencia terapéutica de tabletas de diazepam dispensadas en la ciudad de Ica, Perú.


    Herrera-Calderon, Oscar; Químico Farmacéutico. Facultad de Farmacia y Bioquímica, Universidad Nacional “San Luis Gonzaga”. Ica.; Grande-Ortiz, Miguel; Químico Farmacéutico. Laboratorio de Biodisponibilidad - Bioequivalencia, Centro Nacional de Control de Calidad, Instituto Nacional de Salud. Lima.


    Objetivos: Determinar la equivalencia terapéutica in vitro de tres formulaciones de diazepam 10 mg tabletas, paraestablecer su intercambiabilidad con el medicamento de referencia original. Material y métodos: Estudio descriptivo,comparativo, realizado en junio del 2010, utilizando tres medicamentos genéricos dispensados en la ciudad de Ica yel medicamento original de referencia: Diazepam (Valium®) de laboratorios Roche. Se determinaron los perfiles dedisolución efectuados en medios similares ...

  5. Probing thermonuclear burning on accreting neutron stars


    Keek, L.


    Neutron stars are the most compact stars that can be directly observed, which makes them ideal laboratories to study physics at extreme densities. Neutron stars in low-mass X-ray binaries accrete hydrogen and helium from a lower-mass companion star through Roche lobe overflow. This matter undergoes thermonuclear burning in the neutron star envelope, creating carbon and heavier elements. The fusion process may proceed in an unstable manner, resulting in a thermonuclear runaway. Within one seco...

  6. Mechanism and Therapy for the Shared Susceptibility to Migraine and Epilepsy after Traumatic Brain Injury (United States)


    T-per (Pierce) extraction buffer (150 mg/ml), complemented with a protease (Complete Mini Protease Inhibitor Tablets, Roche) and phosphatase...Gall, C.M., 2010. BDNF signaling during learning is regionally differentiated within hippocampus . J. Neurosci. 30, 15097–15101. Chen, Wang, Y.F...signals. Nature 324, 361–4. Guo, C., Yang, Y., Su, Y., Si, T., 2010. Postnatal BDNF expression profiles in prefrontal cortex and hippocampus of a

  7. Mikroskopische Studien ueber Gesteine aus den Molukken. 1. Gesteine von Ambon und den Uliassern. Mit einem französischen Résumé

    NARCIS (Netherlands)

    Schroeder van der Kolk, J.L.C.


    Dans le traité précédent j’ai décrit les types les plus caractéristiques parmi les échantillons de roche recueillis par M. MARTIN pendant ses voyages dans les Moluques. Ainsi il s’agit des Granites à biotite, des Péridotites, des Dacites, des Andésites à pyroxène, des Brèches volcaniques et aussi de

  8. Introductory note on Emergent Unconscious Knowledge Networks (Asygnodic Networks

    Directory of Open Access Journals (Sweden)

    Henry Bakis


    Full Text Available This note introduces the following paper on the concept of Emergent Unconscious Knowledge Networks (Asygnodic Networks created by E. Roche and M. Blaine. The concept of asyngnosis explains a large number of diverse phenomena involving organizations, groups and decision making. It will present the genesis and definition of Asygnodic Networks and will focus on how they raise challenges to traditional theories of decision making and emerging social networks.

  9. Thermonuclear Burning on the Accreting X-Ray Pulsar GRO J1744-28

    CERN Document Server

    Bildsten, L; Bildsten, Lars; Brown, Edward F.


    We investigate the thermal stability of nuclear burning on the accreting X-ray pulsar GRO J1744-28. The neutron star's dipolar magnetic field is 50 years. We also discuss the nature of the binary and point out that a velocity measurement of the stellar companion (most likely a Roche-lobe filling giant with m_K>17) will constrain the neutron star mass.

  10. Effet Hall quantique, liquides de Luttinger et charges fractionnaires (United States)

    Roche, Patrice; Rodriguez, V.; Glattli, D. Christian

    We review some basic properties of the Fractional Quantum Hall Effect and particularly address the physics of the edge states. The chiral Luttinger liquid properties of the edges are discussed and probed experimentally using transport measurements. Shot noise measurements, which allow determination of the quasiparticle charge are also discussed. To cite this article: P. Roche et al., C. R. Physique 3 (2002) 717-732.

  11. Mid-Infrared Fine-Structure Line Ratios in Active Galactic Nuclei Observed with the Spitzer IRS: Evidence for Extinction by the Torus (United States)


    indicators of the star formation activity in galaxies (e.g., Luhman et al. 2003; Genzel et al. 1998; Roche et al. 1991; Rigopoulou et al. 1999; Clavel et...J. E., & Tielens, A. G. G. M. 2006, ApJ, 637, 774 (CT06) Clavel , J., et al. 2000, A&A, 357, 839 Clegg, R. E. S., Harrington, J. P., Barlow, M. J

  12. A Host Transcriptional Signature for Presymptomatic Detection of Infection in Humans Exposed to Influenza H1N1 or H3N2 (United States)


    intensity [6]. Although previous studies with dengue, melioidosis, tuberculosis, candidiasis , and sepsis have focused on diagnosis in patients as they...the quarantine. All subjects received oral oseltamivir (Roche Pharmaceuticals) 75 mg by mouth twice daily as treatment or prophylaxis at day 6...Lucas J, Perfect JR, Ginsburg GS (2010) Blood gene expression signatures predict invasive candidiasis . Sci Transl Med 2: 21ra17. 8. Berry MP, Graham

  13. Molecular Modeling in Drug Design for the Development of Organophosphorous Antidotes/Prophylactics (United States)


    5012 61102A 1102BS11 EB 025 11. TITLE (Include Security Classification) Molecular Modeling in Drug Design for the Development of Organophosphorous...t ....................................., ’ i.° AD MOLECULAR MODELING IN DRUG DESIGN FOR THE DEVELOPMENT OF ORGANOPHOSPHOROUS ANTIDOTES...Reed, W.J. Murray, E.B. Roche and L.N. Donelsmith, Gen. Pharmac., 12, 177-185 (1981). 5. L.B.Kier, "Molecular Orbital Theory in Drug Design ", Academic

  14. Aspects of computer vision in surgical endoscopy (United States)

    Rodin, Vincent; Ayache, Alain; Berreni, N.


    This work is related to a project of medical robotics applied to surgical endoscopy, led in collaboration with Doctor Berreni from the Saint Roch nursing-home in Perpignan, France). After taking what Doctor Berreni advises, two aspects of endoscopic color image processing have been brought out: (1) The help to the diagnosis by the automatic detection of the sick areas after a learning phase. (2) The 3D reconstruction of the analyzed cavity by using a zoom.

  15. Autonomic Dysreflexia-Like Syndrome in a T12 Paraplegic During Thoracic Spine Surgery (United States)


    or lumbar spinal cord lesions and paraplegia have been reported to exhibit catecholamine-induced hypertension and autonomic dys- function of central...cord origin. Roche et al.17 reported a series of 5 African American male patients with low thoracic or lumbar traumatic paraplegia exhibiting severe...Am Paraplegia Soc 1992;15:171–86 4. Amzallag M. Autonomic hyperreflexia. Int Anesthesiol Clin 1993;31:87–102 5. Karlsson AK. Autonomic dysreflexia

  16. SS433 Trek 2: light curve analysis. (United States)

    Fukue, J.; Obana, Y.; Okugami, M.

    The authors have calculated theoretical light curves of SS433 during eclipse and precession, using a model in which SS433 consists of a geometrically thick torus around a compact star and a companion star filling the Roche lobe. The favorite combination is that the mass ratio is about 2 (a compact star is a black hole) and the surface temperature of the companion is around 17000K.

  17. Malonyl-CoA Decarboxylase (MCD) as a Potential Therapeutic Target for Breast Cancer (United States)


    of detection, using the ATP Bioluminescence Kit CLS II (Roche Diagnostics, Indianapolis, IN, USA) following the manufacturer’s protocol and read on a... ATP levels, and is cytotoxic to MCF7 cells, but not to human fibroblasts. In addition, we s ynthesized a s mall m olecule i nhibitor o f M CD, 5...Figure 1). Acetyl-CoA carboxylase (ACC), the rate limiting enzyme of fatty acid synthesis, produces malonyl-CoA from the ATP dependent carboxylation of

  18. SWIFT J1749.4-2807: A neutron or quark star?

    Institute of Scientific and Technical Information of China (English)

    Jun-Wei Yu; Ren-Xin Xu


    We investigate a unique accreting millisecond pulsar with X-ray eclipses,SWIFT J1749.4-2807(hereafter J1749),and try to set limits on the binary system by various methods including use of the Roche lobe,the mass-radius relations of both main sequence(MS)and white dwarf(WD)companion stars,as well as the measured mass function of the pulsar.The calculations are based on the assumption that the radius of the companion star has reached its Roche radius(or is at 90%),but the pulsar's mass has not been assumed to be a certain value.Our results are as follows.The companion star should be an MS one.For the case that the radius equals its Roche one,we have a companion star with mass M(~-)0.51 M⊙ and radius Rc(~-)0.52 R⊙,and the inclination angle is i(~-)76.5°; for the case that the radius reaches 90% of its Roche one,we have M(~-)0.43 M⊙,Re()0.44 R⊙ and i(~-)75.7°.We also obtain the mass of J1749,Mp(~-)1 M⊙,and conclude that the pulsar could be a quark star if the ratio of the critical frequency of rotation-mode instability to the Keplerian one is higher than~0.3.The relatively low pulsar mass(about~M⊙)may also challenge the conventional recycling scenario for the origin and evolution of millisecond pulsars.The results presented in this paper are expected to be tested by future observations.

  19. Amorphous Fast Ion Conducting Systems, Part 1. Structure and Properties of Mid and Far IR Transmitting Materials, Part 2 (United States)


    of Materials Chemistry and Physics aspect of glassy dynamics, and the absence of a detailed (Vol. 23, nos. 1/2), in ref Ia. We will, however, refer... Chemistry and Physics , 23 (1989) 143-169 143 CORRELATION OF MECHANICAL AND ELECTRICAL RELAXATION PHENOMENA IN SUPERIONIC CONDUCTING GLASSES C.A...Non-Cryst. Solids, 1986 (in press). 24. S. Susman, C. J. Delbecq, J. A. McMillan, and M. F. Roche, Solid State Ionics, 9&10, 667 (1983). 0 Materials

  20. Functional Analysis of the Beclin-1 Tumor Suppressor Interaction With hVps34 (Type-III P13-Kinase) in Breast Cancer Cells (United States)


    1% Nonidet P40 , 0.5% sodium deoxycholate, 0.1% SDS, 5 mM EDTA. Insoluble material was removed by centrifugation at 100,000 g for 45 minutes at 4°C...7.4, 2 mM EDTA, 0.5% Nonidet P-40, 0.1% SDS, 0.5% sodium deoxycholate) supplemented with complete mini protease inhibitors (Roche). Insoluble material

  1. X-ray variability and evidence for pulsations from the unique radio pulsar/X-ray binary transition object FIRST J102347.6+003841

    NARCIS (Netherlands)

    Archibald, A.M.; Kaspi, V.M.; Bogdanov, S.; Hessels, J.W.T.; Stairs, I.H.; Ransom, S.M.; McLaughlin, M.A.


    We report on observations of the unusual neutron-star binary system FIRST J102347.6+003841 carried out using the XMM-Newton satellite. This system consists of a radio millisecond pulsar (PSR J1023+0038) in a 0.198 day orbit with a ~0.2 M sun Roche-lobe-filling companion and appears to have had an ac


    Institute of Scientific and Technical Information of China (English)



    The purpose of this paper is to give a refinement of the Atiyah-Singer families index theorem at the level of differential characters. Also a Riemann-Roch-Grothendieck theorem for the direct image of flat vector bundles by proper submersions is proved,with Chern classes with coefficients in C/Q. These results are much related to prior work of Gillet-Soule, Bismut-Lott and Lott.

  3. Systemic and Pulmonary Hypertension After Resuscitation with Cell-Free Hemoglobin (United States)


    according to published standards (24). Inorganic phosphate was measured on a blood chemistry analyzer (Cobas-Fara; Roche Diagnostic Systems, Nutley, NJ...alanine aminotransferase (ALT), and lactate dehydrogenase (LDH) on the blood chemistry analyzer . The final 4 ml of blood was heparinized, centrifuged...milliliter of blood was placed in 2 ml of ice-cold perchioric acid (70% wt/vol), which was used to determine whole blood lactate in the blood chemistry

  4. Application of next-generation sequencing for comparative transcriptome analysis


    Shin, Heesun


    I have used novel whole transcriptome sequence data generated from massively parallel high-throughput next generation sequencing technologies, namely 454 pyrosequencing and Illumina sequencing, to perform comparative transcriptome analyses of C. elegans populations in specific biological conditions and developmental stages. Firstly, I have conducted transcriptome profiling of C. elegans in its first larval (L1) stage using data generated from the Roche 454 sequencing platform. I have used thi...

  5. Increasing Efficiency and Quality by Consolidation of Clinical Chemistry and Immunochemistry Systems with MODULAR ANALYTICS SWA


    Mocarelli, Paolo; Horowitz, Gary L.; Gerthoux, Pier Mario; Cecere, Rossana; Imdahl, Roland; Ruinemans-Koerts, Janneke; Luthe, Hilmar; Calatayud, Silvia Pesudo; Salve, Marie Luisa; Kunst, Albert; McGovern, Margaret; Ng, Katherine; Stockmann, Wolfgang


    MODULAR ANALYTICS Serum Work Area (in USA Integrated MODULAR ANALYTICS, MODULAR ANALYTICS is a trademark of a member of the Roche Group) represents a further approach to automation in the laboratory medicine. This instrument combines previously introduced modular systems for the clinical chemistry and immunochemistry laboratory and allows customised combinations for various laboratory workloads. Functionality, practicability, and workflow behaviour of MODULAR ANALYTICS Serum Work Area were ev...

  6. Feasibility Analysis For Heating Tribal Buildings with Biomass

    Energy Technology Data Exchange (ETDEWEB)

    Steve Clairmont; Micky Bourdon; Tom Roche; Colene Frye


    This report provides a feasibility study for the heating of Tribal buildings using woody biomass. The study was conducted for the Confederated Salish and Kootenai Tribes of the Flathead Reservation in western Montana. S&K Holding Company and TP Roche Company completed the study and worked together to provide the final report. This project was funded by the DOE's Tribal Energy Program.

  7. Acoustic Metadata Management and Transparent Access to Networked Oceanographic Data Sets (United States)


    Transparent Access to Networked Oceanographic Data Sets Marie A. Roch Dept. of Computer Science San Diego State University 5500 Campanile Drive San...University. In addition to providing database services, the Tethys metadata server also provides access to oceanographic data sets in a consistent...passive acoustic monitoring community. 3 3. Access to network available data products in a standard manner (e.g. ephemeris). 4. Secure access on

  8. Low Diversity in the Mitogenome of Sperm Whales Revealed by Next-Generation Sequencing


    Alexander, Alana; Steel, Debbie; Slikas, Beth; Hoekzema, Kendra; Carraher, Colm; Parks, Matthew; Cronn, Richard; Baker, C. Scott


    Large population sizes and global distributions generally associate with high mitochondrial DNA control region (CR) diversity. The sperm whale (Physeter macrocephalus) is an exception, showing low CR diversity relative to other cetaceans; however, diversity levels throughout the remainder of the sperm whale mitogenome are unknown. We sequenced 20 mitogenomes from 17 sperm whales representative of worldwide diversity using Next Generation Sequencing (NGS) technologies (Illumina GAIIx, Roche 45...

  9. Cobas-6000 - Kliinisen kemian analysaattorin laitekoestus parathormonimäärityksen osalta


    Lehtinen, Otto


    Laitekoestus on analysaattorin toimintakyvyn testaamista. Tutkimuksessa koestettavana analysaattorina oli Roche Cobas-6000. Koestus suoritettiin elektrokemiluminometrisen parathormonimäärityksen osalta. Tarkoituksena oli selvittää, kuinka toistettavia ja täsmääviä tuloksia Cobas-analysaattori antaa ja kuinka hyvin saadut tulokset täsmäävät aiemman palveluntarjoajan, Medixlaboratorion, tulostasoon. Tutkimus määriteltiin kvantitatiiviseksi. Saadut analyysitulokset on sen mukaisesti arvioitu...

  10. Two New Eclipsing Binaries: V1626 Ori (Brh V38) and GSC 0486-4828 (Brh V64) (United States)

    Lloyd, C.; Frank, P.; Bernhard, K.; Moschner, W.; Quester, W.


    V1626 Ori is an EA with a period of 1.137793 days containing a mid F-type main-sequence primary and an oversized early K-type secondary, which may or may not fill its Roche lobe. GSC 0486-4828 has a period of 9.27850 days and contains two apparently unevolved main-sequence stars of spectral types late B and early A.

  11. Avaliação da infestação e diversidade de Teredinidae (Mollusca - Bivalvia) ao longo da costa do estado do Rio de Janeiro, Brasil Evaluation of the infestation and diversity of Teredinidae (Mollusca - Bivalvia) along the coast of Rio de Janeiro State, Brazil


    Junqueira, Andrea O. R.; Sergio Henrique G. da Silva; Maria Júlia M. Silva


    In order to evaluate the diversity and infestation level of Teredinidae wood-boring molluscs, ten satations were chosen along the coast of Rio de Janeiro State. Using five pine-wood panels in each station, it was possible to distinguish five areas according to species dominance. Ilha Grande Bay is characterized by the dominance of Lyrodus floridanus (Bartsch, 1922); Sepetiba Bay, by Bankia fimbriatula Moll & Roch, 1931, Tijuca Lagoon by Teredo navalis Linnaeus, 1758; Guanabara Bay by Notoredo...

  12. Heat Redistribution and Misaligned Orbit Models in PHOEBE (United States)

    Horvat, Martin; Prsa, Andrej; Conroy, Kyle E.


    Reflection and aligned Roche geometry have been long supported in modeling codes that synthesize light and radial velocity curves of eclipsing binary stars. However, recent advances in observational data, mostly in terms of precision and temporal baseline, demonstrated that the assumptions of these two effects are frequently violated. Reflection treatment neglected the energy absorbed by the irradiated star, and Roche geometry assumed aligned vectors of spin and orbital angular momentum. Observations of night- and day-side brightness variation of cooler stellar and substellar companions point to a clear deficiency in treating heat redistribution, and the break in symmetry of the Rossiter-McLaughlin effect points to misaligned stellar spins w.r.t. orbital plane. The framework of existing codes did not allow for revising these effects while keeping the rest of the logic intact, which prompted a complete rewrite of the modeling code PHOEBE (PHysics Of Eclipsing BinariEs). Here we present the basic considerations and proof-of-concept examples of the revised reflection effect and misaligned spin-orbit support. Reflection has been extended with heat absorption and consequent redistribution, which can be local, longitudinal or global. Misaligned spin-orbit vectors are supported by deriving the equation of the Roche potential that allows misaligned rotational axes and are provided by the corresponding Euler angles. This research is supported by the NSF grant #1517474.

  13. Tidal Interactions and Disruptions of Giant Planets on Highly Eccentric Orbits

    CERN Document Server

    Faber, J A; Willems, B; Faber, Joshua A.; Rasio, Frederic A.; Willems, Bart


    We calculate the evolution of planets undergoing a strong tidal encounter using smoothed particle hydrodynamics (SPH), for a range of periastron separations. We find that outside the Roche limit, the evolution of the planet is well-described by the standard model of linear, non-radial, adiabatic oscillations. If the planet passes within the Roche limit at periastron, however, mass can be stripped from it, but in no case do we find enough energy transferred to the planet to lead to complete disruption. In light of the three new extrasolar planets discovered with periods shorter than two days, we argue that the shortest-period cases observed in the period-mass relation may be explained by a model whereby planets undergo strong tidal encounters with stars, after either being scattered by dynamical interactions into highly eccentric orbits, or tidally captured from nearly parabolic orbits. Although this scenario does provide a natural explanation for the edge found for planets at twice the Roche limit, it does no...

  14. In situ cosmogenic 10Be dating of the Quaternary glaciations in the southern Shaluli Mountain on the Southeastern Tibetan Plateau

    Institute of Scientific and Technical Information of China (English)

    WANG; Jian; Raisbeck; Grand; XU; Xiaobin; Yiou; Francios; BAI; Shibiao


    It is generally considered that four-times ice age happened during the Quaternary epoch on the Tibetan Plateau.However,the research on the chronology of the four-times ice age is far from enough.The Shaluli Mountain on the Southeastern Tibetan Plateau is an ideal place for plaeo-glacier study,because there are abundant Quaternary glacial remains there.This paper discusses the ages of the Quaternary glaciations,based on the exposure dating of roche moutonnée,moraines and glacial erosion surfaces using in situ cosmogenic isotopes 10Be.It is found that the exposure age of the roche moutonnée at Tuershan is 15 ka,corresponding to Stage 2 of the deep-sea oxygen isotope,suggesting that the roche moutonnée at Tuershan is formed in the last glacial maximum.The exposure age of glacial erosion surface at Laolinkou is 130-160 ka,corresponding to Stage 6 of the deep-sea oxygen isotope.The oldest end moraine at Kuzhaori may form at 421-766 kaBP,corresponding to Stages 12-18 of the deep-sea oxygen isotope.In accordance with the climate characteristic of stages 12,14,16 and 18 reflected by the deep-sea oxygen isotope,polar ice cores and loess sequence,the oldest end moraine at Kuzhaori may form at stage 12 or stage 16,the latter is more possible.

  15. Instability of mass transfer in a planet-star system

    CERN Document Server

    Jia, Shi


    We show that the angular momentum exchange mechanism governing the evolution of mass transferring binary stars does not apply to Roche-lobe filling planets, because most of the angular momentum of the mass transferring stream is absorbed by the host star. Apart from a correction for the difference in specific angular momentum of the stream and the centre of mass of the planet, the orbit does not expand much on Roche-lobe overflow. We explore the conditions for dynamically unstable Roche-lobe overflow as a function of planet mass and mass and radius (age) of host star and equation of state of planet. For a Sun-like host, gas giant planets in a range of mass and entropy can undergo dynamical mass transfer. Examples of the evolution of the mass transfer process are given. Dynamic mass transfer of rocky planets depend somewhat sensitively on equation of state used. Silicate planets in the range $1 M_{\\oplus}

  16. Mass-Loss Rates of "Hot-Jupiter" Exoplanets with Various Types of Gaseous Envelopes

    CERN Document Server

    Cherenkov, A A; Kaigorodov, P V


    According to the computations results obtained by Bisikalo et al. (2013b) for the gas-dynamical effect of stellar winds on exoplanet atmospheres, three types of gaseous envelopes can form around hot Jupiters: closed, quasi-closed, and open. The type of envelope that forms depends on the position of the frontal collision point (where the dynamical pressure of the wind is equal to the pressure of the surrounding atmosphere) relative to the Roche-lobe boundaries. Closed envelopes are formed around planets whose atmospheres lie completely within their Roche lobes. If the frontal collision point is located outside the Roche lobe, the atmospheric material begins to flow out through the Lagrangian points $\\mathrm{L_1}$ and $\\mathrm{L_2}$, which can result in the formation of quasi-closed (if the dynamical pressure of the stellar wind stops the outflow through $\\mathrm{L_1}$) or open gaseous envelopes. The example of the typical hot Jupiter HD 209458 b is considered for four sets of atmospheric parameters, to determi...

  17. Formation of Janus and Epimetheus from Saturn's rings as coorbitals, thanks to Mimas' 2:3 inner Mean Motion Resonances (United States)

    Crida, Aurelien; El Moutamid, Maryame


    Janus and Epimetheus orbit Saturn at 151461 km on average, on mutual horseshoe orbits with orbital separation 50 km, exchanging position every 4 years. This configuration is unique and intriguing : Lissauer et al. (1985) have shown that their orbital separation should converge to zero in about 20 Myrs only, and no satisfactory model for the origin of this co-orbital resonance exists yet.Charnoz et al. (2010) have shown that Janus and Epimetheus probably formed from the spreading of the rings beyond the Roche radius. Here, we show that this happened when Mimas' 2:3 Lindblad Resonance, which used to confine the rings, receded past the Roche radius. This first explains the gap in mass and distance between Janus and Mimas, which is unexpected in the pyramidal regime of the ring spreading model (Crida & Charnoz 2012). Furthermore, at this time, the two capture sites of Mimas's 2:3 Corotation Resonance were full of ring material. We suggest that as the two capture sites were brought beyond the Roche radius, the captured material agglomerated into two bodies of ~1015 kg on the exact same orbit. These bodies then migrated outwards together due to their interaction with the rings, in mutual horseshoe orbits. The rings then spawn new small satellites, eventually accreted by the proto-Janus and the proto-Epimetheus. This excites their orbital separation, leading to today's configuration.

  18. A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

    Directory of Open Access Journals (Sweden)

    Mahe YF


    Full Text Available Yann F Mahe,1 Marie-Jesus Perez,1 Charlotte Tacheau,1 Chantal Fanchon,2 Richard Martin,3 Françoise Rousset,1 Sophie Seite4 1L’Oreal Research and Innovation, Clichy, 2L’Oréal Research and Innovation, Chevilly Larue, 3L’Oréal Research and Innovation Tours, 4La Roche-Posay Pharmaceutical Laboratories, Asnières, France Abstract: Vitreoscilla filiformis (VF biomass (VFB has been widely used in cosmetic preparations and shown to modulate the major inducible free-radical scavenger mitochondrial superoxide dismutase in skin cells. By adding La Roche-Posay (LRP thermal spring water to the VF culture medium, we obtained a biomass (LRP-VFB with a similar mitochondrial superoxide dismutase activation capacity to VF. Also, the new biomass more powerfully stimulated mRNA expression and antimicrobial peptides in reconstructed epidermis. Interestingly, a predictive computer model that analyzed transducing events within skin epidermal cells suggested that this protective activity may involve the Toll-like receptor 2/protein kinase C, zeta transduction pathway. Protein kinase C, zeta inhibition was effectively shown to abolish VFB-induced gene stimulation and confirmed this hypothesis. This thus opens new avenues for investigation into the improvement of skin homeostatic defense in relation to the control of its physiological microbiota and innate immunity. Keywords: innate skin defenses, TLR2, PKCz, La Roche-Posay, mitochondrial superoxide dismutase, SOD2

  19. Fozivudine: BM 211290, fozivudine tidoxil, FZT, HDP 990002, W 09726867. (United States)


    Fozivudine [BM 211290, fozivudine tidoxil, FZT, HDP 990002, W 09726867] is a thioether lipid conjugate of zidovudine (AZT) in clinical development with Heidelberg Pharma Holdings for the potential treatment of HIV infection. Heidelberg Pharma's proprietary Linker-Cleavag-Enzyme Technology (LCE_Technology) is used to conjugate specific carriers to parent compounds, significantly improving their pharmacological, toxicological and/or clinical profile. LCE_Technology was originated at Boehringer Mannheim, which was acquired by, and integrated into, Roche. In 1999, when Heidelberg Pharma was founded, following a management buy-out from Roche Diagnostics GmbH, the new company acquired full ownership of the intellectual property and documentation related to various proprietary compounds, the LCE_Technology and its applications. According to Heidelberg Pharma's pipeline, published in August 2003, the product candidate, HDP 99.0002 (the company-specific code for fozivudine) will be in phase III development until early 2006, at which time the company hopes to file an NDA. A phase II study of fozivudine in 72 antiretroviral-naive patients with HIV infection was completed in the US and France by Roche.

  20. Mass-Loss Evolution of Close-In Exoplanets: Evaporation of Hot Jupiters and the Effect on Population

    CERN Document Server

    Kurokawa, Hiroyuki


    During their evolution, short-period exoplanets may lose envelope mass through atmospheric escape owing to intense XUV (X-ray and extreme ultraviolet) radiation from their host stars. Roche-lobe overflow induced by orbital evolution or intense atmospheric escape can also contribute to mass loss. To study the effects of mass loss on inner planet populations, we calculate the evolution of hot Jupiters considering mass loss of their envelopes and thermal contraction. Mass loss is assumed to occur through XUV-driven atmospheric escape and the following Roche-lobe overflow. The runaway effect of mass loss results in a dichotomy of populations: hot Jupiters that retain their envelopes and super Earths whose envelopes are completely lost. Evolution primarily depends on the core masses of planets and only slightly on migration history. In hot Jupiters with small cores (~10 Earth masses), runaway atmospheric escape followed by Roche-lobe overflow may create sub-Jupiter deserts, as observed in both mass and radius dist...

  1. Instability of mass transfer in a planet-star system (United States)

    Jia, Shi; Spruit, H. C.


    We show that the angular momentum exchange mechanism governing the evolution of mass-transferring binary stars does not apply to Roche lobe filling planets, because most of the angular momentum of the mass-transferring stream is absorbed by the host star. Apart from a correction for the difference in specific angular momentum of the stream and the centre of mass of the planet, the orbit does not expand much on Roche lobe overflow. We explore the conditions for dynamically unstable Roche lobe overflow as a function of planetary mass and mass and radius (age) of host star and equation of state of planet. For a Sun-like host, gas giant planets in a range of mass and entropy can undergo dynamical mass transfer. Examples of the evolution of the mass transfer process are given. Dynamic mass transfer of rocky planets depends somewhat sensitively on equation of state used. Silicate planets in the range 1 mass transfer before settling to slow overflow when their mass drops to less than 1 M⊕.

  2. Disk Accretion Onto High-Mass Planets

    CERN Document Server

    Lubow, S H; Artymowicz, P


    We analyze the nonlinear, two-dimensional response of a gaseous, viscous protoplanetary disk to the presence of a planet of one Jupiter mass (1 M_J) and greater that orbits a 1 solar mass star by using the ZEUS hydrodynamics code with high resolution near the planet's Roche lobe. The planet is assumed to be in a circular orbit about the central star and is not allowed to migrate. A gap is formed about the orbit of the planet, but there is a nonaxisymmetric flow through the gap and onto the planet. The gap partitions the disk into an inner (outer) disk that extends inside (outside) the planet's orbit. For a 1 M_J planet and typical disk parameters, the accretion through the gap onto the planet is highly efficient. For typical disk parameters, the mass doubling time scale is less than 10^5 years, considerably shorter than the disk lifetime. Following shocks near the L1 and L2 Lagrange points, disk material enters the Roche lobe in the form of two gas streams. Shocks occur within the Roche lobe as the gas stream...

  3. Nature of the Extreme Ultraluminous X-ray Sources

    CERN Document Server

    Wiktorowicz, Grzegorz; Sadowski, Aleksander; Belczynski, Krzysztof


    In this proof-of-concept study we demonstrate how a binary system can easily form an extreme ULX source with the X-ray luminosity of L_x > 10^42 erg/s. Formation efficiencies and lifetimes of such objects are high enough to potentially explain all observed extreme ULXs. These systems are not only limited to binaries with stellar-origin black hole accretors. Noteworthy, we have also identified such objects with neutron stars. Typically, a 10 Msun black hole is fed by a massive (~10 Msun) Hertzsprung gap donor with Roche lobe overflow rate of ~10^-3 Msun/yr (~2600 Mdot_Edd). For neutron star systems the typical donors are evolved low-mass (~2 Msun) helium stars with Roche lobe overflow rate of ~10^-2 Msun/yr. We base our study purely on the available Roche lobe overflow rate in a binary system and show that if only even a small fraction (>10^-3) of the overflow reaches the BH, the source will be super-Eddington. Our study does not prove that any particular extreme ULX (e.g., HLX-1) is a regular binary system wi...

  4. LUT observations of the mass-transferring binary AI Dra (United States)

    Liao, Wenping; Qian, Shengbang; Li, Linjia; Zhou, Xiao; Zhao, Ergang; Liu, Nianping


    Complete UV band light curve of the eclipsing binary AI Dra was observed with the Lunar-based Ultraviolet Telescope (LUT) in October 2014. It is very useful to adopt this continuous and uninterrupted light curve to determine physical and orbital parameters of the binary system. Photometric solutions of the spot model are obtained by using the W-D (Wilson and Devinney) method. It is confirmed that AI Dra is a semi-detached binary with secondary component filling its critical Roche lobe, which indicates that a mass transfer from the secondary component to the primary one should happen. Orbital period analysis based on all available eclipse times suggests a secular period increase and two cyclic variations. The secular period increase was interpreted by mass transfer from the secondary component to the primary one at a rate of 4.12 ×10^{-8}M_{⊙}/yr, which is in agreement with the photometric solutions. Two cyclic oscillations were due to light travel-time effect (LTTE) via the presence of two cool stellar companions in a near 2:1 mean-motion resonance. Both photometric solutions and orbital period analysis confirm that AI Dra is a mass-transferring binary, the massive primary is filling 69 % of its critical Roche lobe. After the primary evolves to fill the critical Roche lobe, the mass transfer will be reversed and the binary will evolve into a contact configuration.

  5. The history of benzodiazepines. (United States)

    Wick, Jeannette Y


    After more than 50 years of experience with benzodiazepines, the American health care system has a love-hate relationship with them. In 1955, Hoffmann-La Roche chemist Leo Sternbach serendipitously identified the first benzodiazepine, chlordiazepoxide (Librium). By 1960, Hoffmann-La Roche marketed it as Librium, and it pursued molecular modifications for enhanced activity. Valium (diazepam) followed in 1963. Hoffmann-La Roche's competitors also began looking for analogues. Initially, benzodiazepines appeared to be less toxic and less likely to cause dependence than older drugs. A specific improvement was their lack of respiratory depression, a safety concern with barbiturates. Medical professionals greeted benzodiazepines enthusiastically at first, skyrocketing their popularity and patient demand. In the mid-to-late 1970s, benzodiazepines topped all "most frequently prescribed" lists. It took 15 years for researchers to associate benzodiazepines and their effect on gamma-aminobutyric acid as a mechanism of action. By the 1980s, clinicians' earlier enthusiasm and propensity to prescribe created a new concern: the specter of abuse and dependence. As information about benzodiazepines, both raising and damning, accumulated, medical leaders and legislators began to take action. The result: individual benzodiazepines and the entire class began to appear on guidelines and in legislation giving guidance on their use. Concurrently, clinicians began to raise concerns about benzodiazepine use by elderly patients, indicating that elders'lesser therapeutic response and heightened sensitivity to side effects demanded prescriber caution. The benzodiazepine story continues to evolve and includes modern-day issues and concerns beyond those ever anticipated.

  6. Factors influencing a low rate of hepatitis C viral RNA clearance in heroin users from Southern China

    Institute of Scientific and Technical Information of China (English)

    ShengHan Lai; Jin-Bing Zhang; Wei Liu; Jie Chen; Xiao-Fang Yu


    AIM: To study the virological and host factors influencing hepatitis C infection outcomes in heroin users in southern China.METHODS: HCV RNA and associated factors were analyzed among 347 heroin users from Guangxi Zhuang Autonomous Region, southern China who were hepatitis C virus (HCV) EIA positive for two or more consecutive visits.RESULTS: Using the COBAS AMPLICOR HCV TEST, a remarkably low HCV RNA negative rate of 8.6% was detected. After multivariate logistic regression analysis, HCV RNA clearance was significantly associated with the presence of HBsAg (OR = 8.436, P < 0.0001), the lack of HIV-1 infection (OR = 0.256, P = 0.038) and age younger than 25 (OR = 0.400, P = 0.029).CONCLUSION: Our study suggests HCV infection among Chinese heroin users results in high levels of viral persistence even amidst factors previously found to enhance viral clearance. Prospective studies of a possible genetic component within the Chinese population and the pathogenicity of non-genotype 1 HCV infections are needed.

  7. Occult hepatitis B in HIV-HCV coinfected patients. (United States)

    Piroth, Lionel; Lafon, Marie-Edith; Binquet, Christine; Bertillon, Pascale; Gervais, Anne; Lootvoet, Enguerrand; Lang, Jean-Marie; De Jaureguiberry, Jean Pierre; Chene, Geneviève; Leport, Catherine


    The prevalence of occult hepatitis B infection in HIV infected patients is controversial, varying from less than 1% to 62% in different studies. Blood samples of 111 HIV-infected patients, HCV-positive, HBs antigen negative, followed in the APROCO-ANRS EP11 cohort, were used to detect HBV DNA by using 2 different validated assays (Cobas Amplicor HBV Monitor Test and INSERM U271 qualitative ultra-sensitive PCR), completed when positive by HBV real-time PCR. HBV DNA was found in 6 (5.4%, 95% CI 1.2%-9.6%) patients by at least 1 of these assays, but none tested positive in all 3 assays. All 6 patients had anti-HBc without anti-HBs antibodies; 5 were not on lamivudine. Their median CD4 and CD8 counts were significantly lower and their HIV viral load higher than in the other 105 patients. In conclusion, the prevalence of occult hepatitis B may vary significantly according to the molecular assay used, even though these assays are validated with high specificity and quite high sensitivity. Occult hepatitis B may be encountered in HIV-HCV coinfected patients without anti-HBV treatment, with anti-HBc but without anti-HBs antibodies, and relatively low immunity, suggesting a potential risk of further reactivation, as already sporadically reported.

  8. [Clinical benefit of HCV core antigen assay in patients receiving interferon and ribavirin combination therapy]. (United States)

    Higashimoto, Makiko; Takahashi, Masahiko; Jokyu, Ritsuko; Saito, Hidetsugu


    A highly sensitive second generation HCV core antigen assay has recently been developed. We compared viral disappearance and kinetics data between commercially available core antigen assays, Lumipulse Ortho HCV Ag, and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV Monitor Test, Version 2 to estimate the predictive benefit of sustained viral response (SVR) and non-SVR in 59 patients treated with interferon and ribavirin combination therapy. We found a good correlation between HCV core Ag and HCV RNA level regardless of genotype. Although the sensitivity of the core antigen assay was lower than PCR, the dynamic range was broader than that of the PCR assay, so that we did not need to dilute the samples in 59 patients. We detected serial decline of core Ag levels in 24 hrs, 7 days and 14 days after interferon combination therapy. The decline of core antigen levels was significant in SVR patients compared to non-SVR as well as in genotype 2a, 2b patients compared to 1b. Core antigen-negative on day 1 could predict all 10 SVR patients (PPV = 100%), whereas RNA-negative could predict 22 SVR out of 25 on day 14 (PPV = 88.0%). None of the patients who had detectable serum core antigen on day 14 became SVR(NPV = 100%), although NPV was 91.2% on RNA negativity. An easy, simple, low cost new HCV core antigen detecting system seems to be useful for assessing and monitoring IFN treatment for HCV.

  9. [Prevalence of hepatitis C virus and excessive consumption of alcohol in a nonhospital worker population]. (United States)

    Prieto Domingo, J J; Carrión Bolaños, J A; Bandrés Moya, F


    The aim of the study was to know the prevalence of the hepatitis C virus (HCV) in a non hospital work population by ELISA 3.0 and PCR-Amplicor, as well as its relationship with excessive alcohol intake (more than 280 g/week in men and 168 g/week in women). A transversal seroepidemiologic study was carried out in 1,109 workers of the Empresa Nacional de Electricidad, S.A. (ENDESA). During the annual medical examinations (April 1993-October 1994) the amount of alcoholic beverages each worker had consumed over the 7 days prior to the medical examination was obtained by anamnesis together with a blood sample for different laboratory tests. Sixteen percent of the workers had had excessive alcohol intake. The prevalence of anti HCV antibodies in the study population was 2.4% being up to 4.6% in the workers declaring excessive alcohol consumption and 10.4% if they also presented an elevation in any of the transaminases. The prevalence of the potentially ineffective workers was 1.46%. The prevalence of anti C antibodies by ELISA 3.0 was greater than expected (2.4%) significantly increasing in the population group which declared excessive alcohol intake, thereby demonstrating the relationship between alcohol and hepatitis C.

  10. The impact of polymerase chain reaction assays for the detection of hepatitis C virus infection in a hemodialysis unit. (United States)

    Hussein, Magdi M; Mooij, Jaap M; Hegazy, Mohamed S; Bamaga, Mohammed S


    Hepatitis C virus (HCV) infection is most often diagnosed by detection of antibodies against the virus (HCV Ab). However, it has been reported that some HCV Ab negative patients test positive for HCV-RNA. Over a study period of 30 months, all patients on hemodialysis at the Al Hada Armed Forces Hospital in Taif, Saudi Arabia were tested monthly for HCV Ab and twice per year for HCV-RNA. HCV Ab was tested by a third generation microparticle enzyme immunoassay (MEIA), and HCV-RNA by a qualitative hepatitis-RNA assay, second version (COBAS Amplicor PCR), which was recently introduced in the Molecular Pathology Laboratory of our hospital. Of the 180 patients studied, 34 (18.9%) had positive HCV Ab, and of the 146 HCV Ab negative patients, five patients tested positive for HCV-RNA (3.42%). Our study further finds that, when applying HCV Ab testing only, some patients with HCV viremia may be undetected. For better HCV infection control, routine HCV-RNA testing of dialysis patients should be considered, particularly in areas where the infection is common and in units applying isolation policies.

  11. Analysis of HCV genotypes from blood donors shows three new HCV type 6 subgroups exist in Myanmar.

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    Shinji T


    Full Text Available The prevalence of hepatitis C virus (HCV genotypes in Myanmar in comparison with the rest of Southeast Asia is not well known. Serum samples were obtained from 201 HCV antibody-positive volunteer blood donors in and around the Myanmar city of Yangon. Of these, the antibody titers of 101 samples were checked by serial dilution using HCV antibody PA test II and Terasaki microplate as a low-cost method. To compare antibody titers by this method and RNA identification, we also checked HCV-RNA using the Amplicor 2.0 test. Most high-titer groups were positive for HCV-RNA. Of the 201 samples, 110 were successfully polymerase chain reaction (PCR amplified. Among them, 35 (31.8% were of genotype 1, 52 (47.3% were of genotype 3, and 23 (20.9% were of type 6 variants, and phylogenetic analysis of these type 6 variants revealed that 3 new type 6 subgroups exist in Myanmar. We named the subgroups M6-1, M6-2, and M6-3. M6-1 and M6-2 were relatively close to types 8 and 9, respectively. M6-3, though only found in one sample, was a brand-new subgroup. These subtypes were not seen in Vietnam, where type 6 group variants are widely spread. These findings may be useful for analyzing how and when these subgroups were formed.

  12. Reference intervals for common thyroid function tests, during different stages of pregnancy in Chinese women

    Institute of Scientific and Technical Information of China (English)

    FAN Jian-xia; HAN Mi; TAO Jun; LUO Jun; SONG Meng-fan; YANG Shuai; KHOR Shu-zin


    Background The importance of diagnosis of thyroid dysfunction during pregnancy has been widely recognized.Our study was designed to compare two different detection reagents between Abbott and Roche and to establish the gestational-related reference intervals for thyroid function tests (TFT) in Chinese women and to assay the reference ranges with the American Thyroid Association recommended standard.Methods Serum samples were collected from 693 normal pregnant Chinese women and divided into five groups according to their gestational age:9-13,16-20,24-28,32-34 and 37-40 weeks.Thyroid stimulating hormone (TSH) and free thyroxine (FT4) levels were determined by two different detection reagents:Abbott Architect l 2000 and Roche Cobas Elecsys 600.The reference ranges of the TFT indexes were calculated according to the National Academy of Clinical Biochemistry (NACB).The 2.5th and 97.5th percentiles of each stage were calculated,and the results were analyzed by one-way analysis of variances,t-test,and Spearman correlation analysis.Results Thyroid hormone levels varied greatly among different gestational stages.TSH levels,as assessed via two different TSH ELISA kits showed consistent changing pattern during pregnancy and displayed linear correlation (P <0.001).In 9-13 gestational weeks,TSH levels were significantly lower than that of other groups; and in 37-40 gestational weeks,it was higher than that of other groups (all P <0.001).TSH reference ranges determined by Roche detection reagent in each group were higher than those by Abbott detection reagent (P <0.01 respectively).FT4 levels were higher in 9-13 gestational weeks than that of other groups (P <0.001).FT4 levels determined by Roche reagent were higher than Abbott reagent in 9-13 weeks,(P <0.001),and lower in 24-28 and 37-40 weeks (P <0.001 and P=0.016,respectively).The TSH level was correlated with FT4 levels in 9-13 gestational weeks by detection reagents (for Abbott reagent,r=-0.319 for FT4 P <0

  13. 床旁血气分析仪与生化仪测定动静脉血钾浓度差异的检测%Detection of Potassium Difference Between Arterial and Venous Blood Measured by Bedside Blood Gas Analyzer and Automatic Biochemical Analyzer

    Institute of Scientific and Technical Information of China (English)

    江龙; 李鹏


    Objective To explore the difference between blood potassium measured by a bedside blood gas analyzer (Abbott i-STAT) and an automatic biochemical analyzer (ROCHE DDP). Methods In this study, arterial and venous blood samples are collected from 130 patients admitted to the intensive care unit (ICU). The concentrations of potassium and pHs in arterial samples are detected by Abbott i-STAT, while the concentrations of serum potassium in paired venous samples are detected by ROCHE DDP. All samples are divided into three groups. The difference between arterial and venous values are calculated according to different pH groups and made for analysis of variance. Results There is significant difference between results of blood potassium of Abbott i-STAT and ROCHE DDP (P<0.05). Blood potassium measured by the two devices is gradually rising when the pH is decreasing. There is no significant difference of blood potassium under different pH between the two groups. Conclusion The arterial potassium tested by bedside blood gas analyzer could not replace the venous potassium tested by biochemical analyzer, pH can affect the value of blood potassium, but the pH would not enlarge the difference of blood potassium measured by two methods above.%目的 分别采用床旁血气分析仪(Abbott i-STAT)和全自动生化仪(ROCHE DDP)测定重症患者动脉全血及静脉血清中的钾离子,分析在不同pH值下两种方法测定钾离子浓度的差异.方法同时采集130例ICU患者的动静脉血标本,采用Abbott i-STAT分析动脉全血的pH值和钾离子,ROCHE DDP测定静脉血清钾.按pH值分组计算动静脉血钾浓度差值,分析不同pH值情况下两种方法检测血钾的差异.结果 Abbott i-STAT测定动脉全血钾在各组均低于ROCHE DDP测定的静脉血清钾,差异有统计学意义(P <0.05).随着pH的降低,测得的动静脉钾离子浓度升高,但其浓度差在组间无统计学差异.结论床旁血气分析仪测得的动脉全血钾不

  14. Asian Highlands Perspectives 10

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    Full Text Available The third volume of collected essays from AHP includes ten new articles, one essay in the Mongghul language, three original short stories, and five folktales. The A mdo Tibetan Lab rtse Ritual by Kelsang Norbu Childbirth and Childcare in Rdo sbis Tibetan Township by Klu mo tshe ring and Gerald Roche Dmu rdo: A Powerful Hero and Mountain Deity by G.yung 'brug and Rin chen rdo rje Echoes from Si gang lih: Burao Yilu's 'Moon Mountain'by Mark Bender The Failure of Vocational Training in Tibetan Areas of China by Shiyong, Wang Fuel and Solar Cooker Impact in Ya na gdung Village, Gcan tsha County, Mtsho sngon (Qinghai Province by Rdo rje don 'grub "I, Ya ri a bsod, Am a Dog": The Life and Music of a Tibetan Mendicant Singer by Skal dbang skyid, Sha bo don sgrub rdo rje, Sgrol ma mtsho, Gerald Roche, Eric Schweickert, and Dpa' rtse rgyal Purity and Fortune in Phug sde Village Rituals by Sa mtsho skyid and Gerald Roche Rgyas bzang Tibetan Tribe Hunting Loreby Bkra shis dpal 'bar sa.bə: A Tibetan Rite of Passageby Lhundrom Muulasan Mongghulby Limusishiden Story - Fateby Gelsang Lhamu Story - A Stolen Journey by Blo bzang tshe ring Story - Is It Karma?by Pad ma rgya mtsho Folklore - Bear and Rabbit (Iby G.yu lha Folklore - Bear and Rabbit (IIby Snying dkar skyid Folklore - The Frog Boy and His Family by Chodpaylhamo Folklore - Mchig nges and Repaying a Debt of Gratitude by Zla ba sgrol ma

  15. Tidal stripping of stars near supermassive black holes

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    Blandford R.


    Full Text Available In a binary system composed of a supermassive black hole and a star orbiting the hole in an equatorial, circular orbit, the stellar orbit will shrink due to the action of gravitational radiation, until the star fills its Roche lobe outside the Innermost Stable Circular Orbit (ISCO of the hole or plunges into the hole. In the former case, gas will flow through the inner Lagrange point (L1 to the hole. If this tidal stripping process happens on a time scale faster than the thermal time scale but slower than the dynamical time scale, the entropy as a function of the interior mass is conserved. The star will evolve adiabatically, and, in most cases, will recede from the hole while filling its Roche lobe. We calculate how the stellar equilibrium properties change, which determines how the stellar orbital period and mass-transfer rate change through the “Roche evolution” for various types of stars in the relativistic regime. We envisage that the mass stream eventually hits the accretion disc, where it forms a hot spot orbiting the hole and may ultimately modulate the luminosity with the stellar orbital frequency. The ultimate goal is to probe the mass and spin of the hole and provide a test of general relativity in the strong-field regime from the resultant quasi-periodic signals. The observability of such a modulation is discussed along with a possible interpretation of an intermittent 1 hour period in the X-ray emission of RE J1034+ 396.

  16. Asian Highlands Perspectives 1

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    Full Text Available •\tA mdo Tibetan Tongue Twisters (7-51 Authors: Blo rtan rdo rje, Charles Kevin Stuart, and Gerald Roche •\tAn Amdo Tibetan Village New Year Trance Medium Ritual (53-64 Authors: Dpal ldan bkra shis, and Charles Kevin Stuart •\tCalling Back the Lost Namuyi Tibetan Soul (65-115 Authors: Libu Lakhi, Charles Kevin Stuart, and Gerald Roche •\tDying Hunters, Poison Plants, and Mute Slaves-Nature and Tradition in Contemporary Nuosu Yi Poetry (117-158 Author: Bender, Mark •\tThe Ersu Shaba Pictographic Writing System (159-186 Author: Sun, Hongkai •\tThe Fourth Belmang-Bodhisattva, Estate Lord, Tibetan Militia Leader, and Chinese Government Official (187-211 Author: Nietupski, Paul K. •\tThe Horse With Two Saddles-Tamxhwe in Modern Golok (213-235 Author: Pirie, Fernanda •\tSeating, Money, and Food at an Amdo Village Funeral (237-294 Authors: Rin chen rdo rje, and Charles Kevin Stuart •\tThe Sengze Village Mani (295-312 Authors: Dkon mchog dge lugs, and Charles Kevin Stuart •\tTibetan Life and Tibetological Discourse-Differences and Recommendations (313-329 Author: Roche, Gerald, Nag tshang grub rgyal, and Mtsho mo skyid •\tA Response to Ways and the Syntax of Noun Phrases in Qinghai Chinese Dialects (331-347 Author: Dede, Keith •\tLazi (Lab rtse Construction in Karmatang (Skar ma thang Village (349-366 Author: Tshe mdo •\tReview-Wutun (367-371 Author: Slater, Keith •\tStory-God Door (373-382 Author: Tshe dpag rnam rgyal

  17. Crystallization-induced dynamic resolution R-epimer from 25-OCH3-PPD epimeric mixture. (United States)

    Zhang, Sainan; Tang, Yun; Cao, Jiaqing; Zhao, Chen; Zhao, Yuqing


    25-OCH3-PPD is a promising antitumor dammarane sapogenin isolated from the total saponin-hydrolyzed extract of Panax ginseng berry and Panax notoginseng leaves. 20(R)-25-OCH3-PPD was more potent as an anti-cancer agent than 20(S)-25-OCH3-PPD and epimeric mixture of 25-OCH3-PPD. This paper describes the rapid separation process of the R-epimer of 25-OCH3-PPD from its epimeric mixture by crystallization-induced dynamic resolution (CIDR). The optimized CIDR process was based on single factor analysis and nine well-planned orthogonal design experiments (OA9 matrix). A rapid and sensitive reverse phase high-performance liquid chromatographic (HPLC) method with evaporative light-scattering detector (ELSD) was developed and validated for the quantitation of 25-OCH3-PPD epimeric mixture and crystalline product. Separation and quantitation were achieved with a silica column using a mobile phase consisting of methanol and water (87:13, v/v) at a flow rate of 1.0mL/min. The ELSD detection was performed at 50°C and 3L/min. Under conditions involving 3mL of 95% ethanol, 8% HCl, and a hermetically sealed environment for 72h, the maximum production of 25(R)-OCH3-PPD was achieved with a chemical purity of 97% and a total yield of 87% through the CIDR process. The 25(R)-OCH3-PPD was nearly completely separated from the 220mg 25-OCH3-PPD epimeric mixture. Overall, a simple and steady small-batch purification process for the large-scale production of 25(R)-OCH3-PPD from 25-OCH3-PPD epimeric mixture was developed.

  18. Performance of an Early Infant Diagnostic Test, AmpliSens DNA-HIV-FRT, Using Dried Blood Spots Collected from Children Born to Human Immunodeficiency Virus-Infected Mothers in Ukraine (United States)

    Shanmugam, Vedapuri; Azarskova, Marianna; Nguyen, Shon; Hurlston, Mackenzie; Sabatier, Jennifer; Zhang, Guoqing; Osmanov, Saladin; Ellenberger, Dennis; Yang, Chunfu; Vitek, Charles; Liulchuk, Maria; Nizova, Natalya


    An accurate accessible test for early infant diagnosis (EID) is crucial for identifying HIV-infected infants and linking them to treatment. To improve EID services in Ukraine, dried blood spot (DBS) samples obtained from 237 HIV-exposed children (≤18 months of age) in six regions in Ukraine in 2012 to 2013 were tested with the AmpliSens DNA-HIV-FRT assay, the Roche COBAS AmpliPrep/COBAS TaqMan (CAP/CTM) HIV-1 Qual test, and the Abbott RealTime HIV-1 Qualitative assay. In comparison with the paired whole-blood results generated from AmpliSens testing at the oblast HIV reference laboratories in Ukraine, the sensitivity was 0.99 (95% confidence interval [CI], 0.95 to 1.00) for the AmpliSens and Roche CAP/CTM Qual assays and 0.96 (95% CI, 0.90 to 0.98) for the Abbott Qualitative assay. The specificity was 1.00 (95% CI, 0.97 to 1.00) for the AmpliSens and Abbott Qualitative assays and 0.99 (95% CI, 0.96 to 1.00) for the Roche CAP/CTM Qual assay. McNemar analysis indicated that the proportions of positive results for the tests were not significantly different (P > 0.05). Cohen's kappa (0.97 to 0.99) indicated almost perfect agreement among the three tests. These results indicated that the AmpliSens DBS and whole-blood tests performed equally well and were comparable to the two commercially available EID tests. More importantly, the performance characteristics of the AmpliSens DBS test meets the World Health Organization EID test requirements; implementing AmpliSens DBS testing might improve EID services in resource-limited settings. PMID:26447114

  19. Accretion of Saturn’s Inner Mid-sized Moons from a Massive Primordial Ice Ring (United States)

    Salmon, J.; Canup, R. M.


    Saturn’s rings are rock-poor, containing 90%–95% ice by mass. As a group, Saturn’s moons interior to and including Tethys are also about 90% ice. Tethys itself contains 40 % rock. Here we simulate the evolution of a massive primordial ice-rich ring and the production of satellites as ring material spreads beyond the Roche limit. We describe the Roche-interior ring with an analytic model, and use an N-body code to describe material beyond the Roche limit. We track the accretion and interactions of spawned satellites, including tidal interaction with the planet, assuming a tidal dissipation factor for Saturn of Q∼ {10}4. We find that ring torques and capture of moons into mutual resonances produce a system of ice-rich inner moons that extends outward to approximately Tethys’s orbit in 109 years, even with relatively slow orbital expansion due to tides. The resulting mass and semimajor axis distribution of spawned moons resembles that of Mimas, Enceladus, and Tethys. We estimate the mass of rock delivered to the moons by external cometary impactors during a late heavy bombardment. We find that the inner moons receive a mass in rock comparable to their current total rock content, while Dione and Rhea receive an order-of-magnitude less rock than their current rock content. This suggests that external contamination may have been the primary source of rock in the inner moons, and that Dione and Rhea formed from much more rock-rich source material. Reproducing the distribution of rock among the current inner moons is challenging, and appears to require large impactors stochasticity and/or the presence of some rock in the initial ring.

  20. Performance characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA Sequencing System. (United States)

    Kuritzkes, Daniel R; Grant, Robert M; Feorino, Paul; Griswold, Marshal; Hoover, Marie; Young, Russell; Day, Stephen; Lloyd Jr, Robert M; Reid, Caroline; Morgan, Gillian F; Winslow, Dean L


    The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mutations and to determine the effects of RNA extraction methods, anticoagulants, specimen handling, and potentially interfering substances. Samples were plasma obtained from HIV-infected subjects or seronegative plasma to which viruses derived from wild-type and mutant infectious molecular clones (IMC) of HIV-1 were added. Extraction methods tested included standard and UltraSensitive AMPLICOR HIV-1 MONITOR, QIAGEN viral RNA extraction mini kit, and QIAGEN Ultra HIV extraction kit, and NASBA manual HIV-1 quantitative NucliSens. Sequence data from test sites were compared to a "gold standard" reference sequence to determine the percent agreement. Comparisons between test and reference sequences at the nucleotide level showed 97.5 to 100% agreement. Similar results were obtained regardless of extraction method, regardless of use of EDTA or acid citrate dextrose as anticoagulant, and despite the presence of triglycerides, bilirubin, hemoglobin, antiretroviral drugs, HIV-2, hepatitis C virus (HCV), HBV, cytomegalovirus, human T-cell leukemia virus type 1 (HTLV-1), or HTLV-2. Samples with HIV-1 RNA titers of >or=1,000 copies/ml gave consistent results. The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System consistently generate highly accurate sequence data when tested with IMC-derived HIV and patient samples.

  1. Association between ocular bacterial carriage and follicular trachoma following mass azithromycin distribution in The Gambia.

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    Sarah E Burr

    Full Text Available BACKGROUND: Trachoma, caused by ocular Chlamydia trachomatis infection, is the leading infectious cause of blindness, but its prevalence is now falling in many countries. As the prevalence falls, an increasing proportion of individuals with clinical signs of follicular trachoma (TF is not infected with C. trachomatis. A recent study in Tanzania suggested that other bacteria may play a role in the persistence of these clinical signs. METHODOLOGY/PRINCIPAL FINDINGS: We examined associations between clinical signs of TF and ocular colonization with four pathogens commonly found in the nasopharnyx, three years after the initiation of mass azithromycin distribution. Children aged 0 to 5 years were randomly selected from 16 Gambian communities. Both eyes of each child were examined and graded for trachoma according to the World Health Organization (WHO simplified system. Two swabs were taken from the right eye: one swab was processed for polymerase chain reaction (PCR using the Amplicor test for detection of C. trachomatis DNA and the second swab was processed by routine bacteriology to assay for the presence of viable Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus and Moraxella catarrhalis. Prevalence of TF was 6.2% (96/1538 while prevalence of ocular C. trachomatis infection was 1.0% (16/1538. After adjustment, increased odds of TF were observed in the presence of C. trachomatis (OR = 10.4, 95%CI 1.32-81.2, p = 0.03, S. pneumoniae (OR = 2.14, 95%CI 1.03-4.44, p = 0.04 and H. influenzae (OR = 4.72, 95% CI 1.53-14.5, p = 0.01. CONCLUSIONS/SIGNIFICANCE: Clinical signs of TF can persist in communities even when ocular C. trachomatis infection has been controlled through mass azithromycin distribution. In these settings, TF may be associated with ocular colonization with bacteria commonly carried in the nasopharnyx. This may affect the interpretation of impact surveys and the determinations of thresholds for discontinuing mass

  2. Therapy of chronic hepatitis C: Virologic response monitoring

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    Kuljić-Kapulica Nada


    Full Text Available Background/Aim. Virological testing is considered to be essential in the management of hepatitis C virus (HCV infection in order to diagnose infection, and, most importantly, as a quide for treatment decisions and assess the virological response to antiviral therapy. The aim of this study was to determine the rate of a sustained virological response (SVR and various factors associated with response rates in chronic hepatitis C infected patients treated with pegiinterferon alpha (PEGINF and ribavirin (RBV combination therapy. Methods. A total of 34 patients, treated with PEG-IFN and RBV were studied. Serum HCV-RNA was measured before the treatment, 12 weeks following the start of the therapy and 6 weeks after the treatment cessation. SVR was defined as undetectable serum HCV-RNA 6 months of post-treatment follow-up, virologic relapse (VR as relapse of HCV-RNA during the post-treatment follow-up. Serum HCV-RNA was measured with the Cobas Amplicor test. Results. At the end of post-treatment follow-up 19 (55.8% patients demonstrated a SVR. The majority of the patients were genotype 1 (27, and the other were genotype 3 (5 patients and genotype 4 (2 patients. There was VR in 6 patients 6 months after the therapy. In 9 patients HCV-RNA was positive after 12 weeks. Conclusion. We demonstrated that patients with chronic HCV infection can be successfully treated with combination of PEG-INF and RBV. This result emphasizes also that post-treatment follow-up to identify patients with SVR or VR could be important.

  3. Performance Characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA Sequencing System (United States)

    Kuritzkes, Daniel R.; Grant, Robert M.; Feorino, Paul; Griswold, Marshal; Hoover, Marie; Young, Russell; Day, Stephen; Lloyd, Jr., Robert M.; Reid, Caroline; Morgan, Gillian F.; Winslow, Dean L.


    The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mutations and to determine the effects of RNA extraction methods, anticoagulants, specimen handling, and potentially interfering substances. Samples were plasma obtained from HIV-infected subjects or seronegative plasma to which viruses derived from wild-type and mutant infectious molecular clones (IMC) of HIV-1 were added. Extraction methods tested included standard and UltraSensitive AMPLICOR HIV-1 MONITOR, QIAGEN viral RNA extraction mini kit, and QIAGEN Ultra HIV extraction kit, and NASBA manual HIV-1 quantitative NucliSens. Sequence data from test sites were compared to a “gold standard” reference sequence to determine the percent agreement. Comparisons between test and reference sequences at the nucleotide level showed 97.5 to 100% agreement. Similar results were obtained regardless of extraction method, regardless of use of EDTA or acid citrate dextrose as anticoagulant, and despite the presence of triglycerides, bilirubin, hemoglobin, antiretroviral drugs, HIV-2, hepatitis C virus (HCV), HBV, cytomegalovirus, human T-cell leukemia virus type 1 (HTLV-1), or HTLV-2. Samples with HIV-1 RNA titers of ≥1,000 copies/ml gave consistent results. The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System consistently generate highly accurate sequence data when tested with IMC-derived HIV and patient samples. PMID:12682150

  4. Síndrome da meningite asséptica por enterovírus e Leptospira sp em crianças de Salvador, Bahia

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    Silva Hagamenon R.


    Full Text Available Objetivando verificar a freqüência de enterovírus (EV, leptospiras e arbovírus como agentes causais da síndrome da meningite asséptica (SMA, em períodos não-epidêmicos, e comparar os pacientes com e sem diagnóstico etiológico determinado, foram selecionados 112 pacientes de idade entre 3 meses e 15 anos, com suspeita clínica de SMA, referenciados para Hospital Couto Maia, especializado em Doenças Infecciosas e Parasitárias (Salvador, Bahia, Em 44,6% (n=50 a etiologia foi determinada: enterovírus em 37,7% (n=42 dos casos, pelo teste de PCR Amplicor, por cultura do líquor e/ou de fezes; a Leptospira sp. em 7,1% (n=8, pelo método da micro-aglutinação, e nenhum caso de arbovírus foi detectado (inibição da hemaglutinação passiva. Entre os 14 enterovírus dos 22 isolados, foram identificados seis diferentes sorotipos, sendo o Echovirus-4 predominante (27,2%; 6/22 entre outros (Coxsackie B2, B3, B6 e B9; EV 71. Conclui-se que, os enterovírus foram os agentes mais freqüentes, e que a leptospirose deve ser lembrada no diagnóstico diferencial da SMA. Uma vez que as características clínicas e liquóricas dos pacientes dos grupos com e sem determinação do agente etiológico foram semelhantes, pode-se supor que o diagnóstico presuntivo de SMA é de provável etiologia viral ou pela leptospira.

  5. Early diagnosis of in utero and intrapartum HIV infection in infants prior to 6 weeks of age. (United States)

    Lilian, Rivka R; Kalk, Emma; Bhowan, Kapila; Berrie, Leigh; Carmona, Sergio; Technau, Karl; Sherman, Gayle G


    Early initiation of antiretroviral therapy reduces HIV-related infant mortality. The early peak of pediatric HIV-related deaths in South Africa occurs at 3 months of age, coinciding with the earliest age at which treatment is initiated following PCR testing at 6 weeks of age. Earlier diagnosis is necessary to reduce infant mortality. The performances of the Amplicor DNA PCR, COBAS AmpliPrep/COBAS TaqMan (CAP/CTM), and Aptima assays for detecting early HIV infection (acquired in utero and intrapartum) up to 6 weeks of age were compared. Dried blood spots (DBS) were collected at birth and at 2, 4, and 6 weeks from HIV-exposed infants enrolled in an observational cohort study in Johannesburg, South Africa. HIV status was determined at 6 weeks by DNA PCR on whole blood. Serial DBS samples from all HIV-infected infants and two HIV-uninfected, age-matched controls were tested with the 3 assays. Of 710 infants of known HIV status, 38 (5.4%) had in utero (n = 29) or intrapartum (n = 9) infections. By 14 weeks, when treatment should have been initiated, 13 (45%) in utero-infected and 2 (22%) intrapartum-infected infants had died or were lost to follow-up. The CAP/CTM and Aptima assays identified 76.3% of all infants with early HIV infections at birth and by 4 weeks were 96% sensitive. DNA PCR demonstrated lower sensitivities at birth and 4 weeks of 68.4% and 87.5%, respectively. All assays had the lowest sensitivity at 2 weeks of age. CAP/CTM was the only assay with 100% specificity at all ages. Testing at birth versus 6 weeks of age identifies a higher total number of HIV-infected infants, irrespective of the assay.

  6. Human papillomavirus prevalence among indigenous and non-indigenous Australian women prior to a national HPV vaccination program

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    Condon John R


    Full Text Available Abstract Background Indigenous women in Australia have a disproportionate burden of cervical cancer despite a national cervical screening program. Prior to introduction of a national human papilloma virus (HPV vaccination program, we determined HPV genotype prevalence by Indigenous status and residence in remote areas. Methods We recruited women aged 17 to 40 years presenting to community-based primary health services for routine Pap screening across Australia. A liquid-based cytology (LBC cervical specimen was tested for HPV DNA using the AMPLICOR HPV-DNA test and a PGMY09/11-based HPV consensus PCR; positive specimens were typed by reverse hybridization. We calculated age-adjusted prevalence by weighting to relevant population data, and determined predictors of HPV-DNA positivity by age, Indigenous status and area of residence using logistic regression. Results Of 2152 women (655 Indigenous, prevalence of the high-risk HPV genotypes was similar for Indigenous and non-Indigenous women (HPV 16 was 9.4% and 10.5%, respectively; HPV 18 was 4.1% and 3.8%, respectively, and did not differ by age group. In younger age groups, the prevalence of other genotypes also did not differ, but in those aged 31 to 40 years, HPV prevalence was higher for Indigenous women (35% versus 22.5%; P Conclusion Although we found no difference in the prevalence of HPV16/18 among Australian women by Indigenous status or, for Indigenous women, residence in remote regions, differences were found in the prevalence of risk factors and some other HPV genotypes. This reinforces the importance of cervical screening as a complement to vaccination for all women, and the value of baseline data on HPV genotype prevalence by Indigenous status and residence for the monitoring of vaccine impact.

  7. Functional Interactions Between c-Src and HER1 Potentiate Neoplastic Transformation: Implications for the Etiology of Human Breast Cancer (United States)


    Rev 12:255-274, 1983. 1544, 1989. 6. Libermann TA, Nussbaum HR, Razon N, et al. Amplification and 30. Roche S, Koegl M, Barone MV, Roussel MF...results suggest phosphorylations on the receptor (Tyr-845 and Tyr-ll01). that one mechanism by which c-Src could augment the mito - Using stable cell lines...stimulation of a mito - Enzymol. 255, 245-256. genic pathway not involving ERK2. 18. Hanks, S. J., Quinn, A. M. & Hunter, T. (1988) Science 241, These

  8. [Cutaneous melanoma - "black death" of modern times? Traces in contemporary literature]. (United States)

    Bahmer, F A; Bahmer, J A


    Cutaneous melanoma, sometimes labeled as "black skin cancer", is increasing in frequency and becoming a more common literary motive. In US literature, Sylvia Plath and Charles Bukowski depicted melanoma more than 50 years ago, later Stephen King and Thomas C. Boyle. In German literature, Charlotte Roche shortly mentioned this tumor. Jörg Pönnighaus, both poet and dermatologist, intensively deals in his poems with the effects melanoma has on patients and doctors alike. Melanoma definitely is not the "Black Death" of modern times. However, the perception of this tumor as extremely malignant and as life-threatening makes melanoma a metaphor of the deadly danger of cancer.

  9. Characterization of the Discoidin Domain Receptor 2 Kinase as a Novel Therapeutic Target for Squamous Cell Lung Cancer (United States)


    Immunoblots were performed using the Nupage System (Invitrogen) per the manufacturer’s protocol. Cells were lysed in 1% Nonidet P - 40 with protease (Roche...based on genetic lesions. J Clin Invest 2009;119:1727– 40 . 33. Du J, Bernasconi P , Clauser KR, Mani DR, Finn SP, Beroukhim R, et al. Bead-based...DDR2 mutations. DDR2 knockdown was associated with a decrease in both cell proliferation and in markers of DDR2 signaling including p -Src and p -STAT3

  10. Nrdp1-Mediated ErbB3 Increase during Androgen Ablation and its Contribution to Androgen-Independence (United States)


    1% Nonidet P - 40 , protease inhibitor cocktail, 0.5 mM sodium orthovanadate, and 1× serine/threonine phosphatase inhibitor). The cell lysate was...Systems (BAF1129, BAM3481, and BAF1131, respectively). Protease inhibitor cocktail tablets and purifi ed 10% Nonidet P - 40 were purchased from Roche...0.43) to castrated, vehicle treated tumors (1.33 ± 0.26) ( p =0.009), which was reduced 40 % in tumors treated with the drugs in castrated animals (0.8

  11. A New Therapeutic Paradigm for Breast Cancer Exploiting Low Dose Estrogen-Induced Apoptosis (United States)


    Nonidet P - 40 lysis buffer containing 1 mM NaO3VO4 and 1X Complete protease inhibitor tablet (Roche). Whole cell...30 40 50 60 70 R el a ti v e p S 2 l ev el s R el a ti v e P R le v el s ** ** 0 2 4 6 8 10 12 14 16 ** ** ERα β-actin PP2 - + 0 0.2 0.4 0.6...1 l ev el s ** 0 5 10 15 20 25 30 35 40 45 50 R el a ti v e T P 6 3 le v el s **

  12. Targeting Transforming Growth Factor Beta to Enhance the Fracture Resistance of Bone (United States)


    Nonidet P - 40 , and protease inhibitors (Complete ULTRA Protease InhibitorMix- ture Tablets, Roche Applied Science). Nuclear extracts were isolated using...25 30 35 40 45 mg/kg P ea k fo rc e (N ) 6 months 22 months * **(A) 13 C4 @ 5 1D 11 @ 1 1D 11 @ 5 13 C4 @ 5 1D 11 @ 1 1D 11 @ 5 0.0...0.075 0.368 13C4 1D11 0 20 40 60 80 P ea k fo rc e (N ) p =0.0035 0 0.15 0.20

  13. Caracterisation Physique des Sols Camp Militaire de Petawawa (Physical Characterization of the Soil in Military Camp of Petawawa). (United States)


    duction par la percolation des ions ferreux et d’alumine par les eaux de pluie ). La troisi~me strate est la roche-m~re dont les caract~ristiques sont...couche d’humus se situe un sable (7 A 11 pouces) dont la teneur en eau va en augmentant selon la profondeur. C’est dans la basse partie de cet horizon que...beaucoup plus faible, la forte teneur en eau unit surtout les particules lee plus fines pour fournir un milieu relativement glissant. L’horizon sous-jacent

  14. Interest of corrective makeup in the management of patients in dermatology


    Seité S; Deshayes P; Dréno B; Misery L; Reygagne P; Saiag P; Stengel F; Roguedas-Contios AM; Rougier A


    S Seité,1 P Deshayes,2 B Dréno,3 L Misery,4 P Reygagne,5 P Saiag,6 F Stengel,7 AM Roguedas-Contios,4 A Rougier11La Roche-Posay Pharmaceutical Laboratories, Asnières, France; 2Service de Dermatologie, Caen, France; 3Hôtel-Dieu, Nantes, France; 4CHU de Brest-Hôpital Morvan, Brest, France; 5Centre Sabouraud, Hôpital Saint-Louis, Paris, France; 6CHU Ambroise Paré, Boulogne, France; 7Department of Dermatology, Buenos Aires, ...

  15. Effects of rearing density on seabass (Dicentrarchus labrax, L. gene expression

    Directory of Open Access Journals (Sweden)

    M. Saroglia


    Full Text Available Animal welfare involves the subjective feeling of animals and the experience of pleasure, pain, frustration, hunger or other states and it is therefore difficult to define and to measure (Drawkins, 1990. When water quality criteria are respected and feeding restrictions are avoided, fish density may become one of the more relevant factors expected to potentially affect fish welfare. Such high density environment, may lead to a fish conditions that cannot be described or forecast simply with measurement of hematological responses (Roche and Bogé, 1996, in the same time, detection of zootechnical performances, do not provide a specific indications...

  16. The application of adenosine triphosphate bioluminescence assay in the diagnosis of multidrug-resistant ;Mycobacterium tuberculosis%三磷酸腺苷发光法在耐多药结核分枝杆菌诊断中的运用

    Institute of Scientific and Technical Information of China (English)

    刘君; 胡嘉波; 裴豪; 蒯守刚; 陈丽艳


    目的:通过与罗氏固体培养法比较,评估三磷酸腺苷发光法检测耐多药结核分枝杆菌的可行性。方法采用三磷酸腺苷发光法与罗氏固体培养法同时检测和分析149例临床分离的结核分枝杆菌。结果三磷酸腺苷发光法与罗氏固体培养法的一致率为92.6%(138/149),差异无统计学意义(χ2=0.57,P=0.45)。三磷酸腺苷发光法检测时间为(6.6±2.1)d,明显快于传统罗氏固体培养法的28 d(t=422.7,P<0.001)。结论与常规检测方法比较,三磷酸腺苷发光法具有快速、简便、准确性高等优点,对耐多药结核患者的早期诊断和耐药结核分枝杆菌流行的控制有很大帮助,适合实验室开展。%Objective To compare with Roche solid culture method,and to evaluate the feasibility of adenosine triphosphate bioluminescence assay for detecting multidrug-resistant Mycobacterium tuberculosis.Methods By Roche solid culture method and adenosine triphosphate bioluminescence assay,149 clinical isolates of Mycobacterium tuber-culosis were determined and analyzed.Results The coincidence rate of adenosine triphosphate bioluminescence assay with Roche solid culture method was 92.6%(138/149),and the difference had no statistical significance (χ2 =0.57, P=0.45).The detection time of adenosine triphosphate bioluminescence assay was (6.6 ±2.1)d,which was faster than that of Roche solid culture method (28 d,t=422.7,P<0.001).Conclusions Compared with the conventional detection methods,adenosine triphosphate bioluminescence assay is simple,rapid and accurate.It is helpful for detecting multidrug-resistant tuberculosis patients and controlling the prevalence of Mycobacterium tuberculosis.It is suitable for clinical laboratory.

  17. Quasi-quantum groups from strings

    CERN Document Server

    Jureit, J -H


    Motivated by string theory on the orbifold ${\\cal M}/G$ in presence of a Kalb-Ramond field strength $H$, we define the operators that lift the group action to the twisted sectors. These operators turn out to generate the quasi-quantum group $D_{\\omega}[G]$, introduced in the context of conformal field theory by R. Dijkgraaf, V. Pasquier and P. Roche, with $\\omega$ a 3-cocycle determined by a series of cohomological equations in a tricomplex combining de Rham, \\u{C}ech and group cohomologies. We further illustrate some properties of the quasi-quantum group from a string theoretical point of view.

  18. Comparative approach to capture bacterial diversity in coastal waters

    DEFF Research Database (Denmark)

    Na, Hyunsoo; Kim, Ok-Sun; Yoon, Suk-hwan;


    to investigate the bacterial community structure of coastal seawater collected from the Yellow Sea, Korea. For culture-independent studies, we used the latest model pyrosequencer, Roche/454 Genome Sequencer FLX Titanium. Pyrosequencing captured a total of 52 phyla including 27 candidate divisions from the water...... column, whereas the traditional cloning approach captured only 15 phyla including 2 candidate divisions. In addition, of 878 genera retrieved, 92.1% of the sequences were unique to pyrosequencing. For culture-dependent analysis, plate culturing, plate washing, enrichment, and high-throughput culturing...

  19. Development and Evaluation of Up-Converting Phosphor Technology-Based Lateral Flow Assay for Quantitative Detection of NT-proBNP in Blood (United States)

    Hao, Qingfang; Zou, Deyong; Zhang, Xiaoli; Zhang, Liping; Li, Hongmei; Qiao, Yong; Zhao, Huansheng; Zhou, Lei


    A newly assay, up-converting phosphor technology-based lateral flow (UPT-LF) assay, was developed for rapid and quantitative detection of N-terminal fragment of B-type natriuretic peptide precursor (NT-proBNP), one of the most important serum molecular maker of heat failure, in plasma samples as a point of care testing (POCT) method for diagnosis of acute heart failure. Human plasma from 197 patients with acute heart failure and 200 healthy controls was assessed using the UPT-LF assay, in a comparison with a Roche Elecsys assay. The limit of detection of the UPT-LF assay, with a coefficient of variation (CV) of less than 15%, was 116 ng/L, which is lower than the clinical diagnosis cutoff (150 ng/mL). The linear range was 50–35,000 ng/L. The CVs were less than 10% for both UPT-LF and Roche Elecsys assays for plasma samples under different storages, demonstrating the good stability and reproducibility. There are certain linear correlations between the results of UPT-LF and Roche Elecsys assay for EDTA-K2 and heparin-anticoagulated plasma, as well as for serum samples. For UPT-LF assay, there is a significant correlation between the values derived from analysis of EDTA-K2 and heparin-anticoagulated plasma samples (R = 0.995). No statistically significant difference was found between serum and plasma samples for UPT-LF assay. Our results demonstrate that NT-proBNP levels in healthy adults are elevated with age and had a relationship with sex, and with the age increase the NT-proBNP levels of females are significantly higher than those of males (p<0.01). The UPT-LF assay has a high reproducibility, stability, sensitivity, specificity, and is consistent with Roche Elecsys assay, and therefore it could be used as a POCT method for the quantitative detection of NT-proBNP in blood for clinical diagnosis and research of acute heart failure. PMID:28151978

  20. Performance and Verification of a Real-Time PCR Assay Targeting the gyrA Gene for Prediction of Ciprofloxacin Resistance in Neisseria gonorrhoeae. (United States)

    Hemarajata, P; Yang, S; Soge, O O; Humphries, R M; Klausner, J D


    In the United States, 19.2% of Neisseria gonorrhoeae isolates are resistant to ciprofloxacin. We evaluated a real-time PCR assay to predict ciprofloxacin susceptibility using residual DNA from the Roche Cobas 4800 CT/NG assay. The results of the assay were 100% concordant with agar dilution susceptibility test results for 100 clinical isolates. Among 76 clinical urine and swab specimens positive for N. gonorrhoeae by the Cobas assay, 71% could be genotyped. The test took 1.5 h to perform, allowing the physician to receive results in time to make informed clinical decisions.

  1. Specificity of the Linear Array HPV Genotyping Test for detecting human papillomavirus genotype 52 (HPV-52)


    Kocjan, Boštjan; Poljak, Mario; Oštrbenk, Anja


    Introduction: HPV-52 is one of the most frequent human papillomavirus (HPV) genotypes causing significant cervical pathology. The most widely used HPV genotyping assay, the Roche Linear Array HPV Genotyping Test (Linear Array), is unable to identify HPV- 52 status in samples containing HPV-33, HPV-35, and/or HPV-58. Methods: Linear Array HPV-52 analytical specificity was established by testing 100 specimens reactive with the Linear Array HPV- 33/35/52/58 cross-reactive probe, but not with the...

  2. Cell Source and Mechanism of Hair Cell Regeneration in the Neonatal Mouse Cochlea (United States)


    recombinase with a modified tamoxifen-inducible estrogen receptor (CreERTM or CreER,.,) (reviewed in’) . Tetracycline-inducible mouse models (Tet-On or...cessation of doxycycline treatment at P2 28• Analogous to the blood-brain barrier, the blood-labyrinth barrier functions to protect the inner ear from...using alkaline phosphatase staining (NBT/BCIP ready to use tablets (Roche cat#ll697471001)). Temporal bones were post-fixed for I hour in 4

  3. 美国消费者组织要求将奥利司他撤出市场

    Institute of Scientific and Technical Information of China (English)


    美国公众消费者组织Public Citizen向美国食品药品监督管理局(FDA)提出申请,希望立即从市场撤出罗氏大药厂(Roche Holding AG’s)生产的奥利司他(orlistat,商品名:Xenical),因为动物实验表明奥利司他与结肠癌前病变有关。该组织还要求FDA不要批准该药作为非处方药销售。

  4. Analytical performance specifications: relating laboratory performance to quality required for intended clinical use. (United States)

    Dalenberg, Daniel A; Schryver, Patricia G; Klee, George G


    This article proposes analytic performance goals for five quality indicators: precision, trueness, linearity, detection limits, and consistency across instruments and time. We defined our goals using methods linked to clinical practice data. Goals for desirable precision and trueness are based on biological variation. Linearity goals are related to total error recommendations. Detection limit goals are derived from 0.1 percentile of patient values. Goals for consistency are derived from the variability of distributions of patient test values. Data were collected and evaluated for each of these quality indicators for 46 chemistry tests measured on the Roche cobas 8000 analyzer.

  5. Autotrophic potential in mesophilic heterotrophic bacterial isolates from Sino-Pacific marine sediments

    Digital Repository Service at National Institute of Oceanography (India)

    Cao, W.; Das, A.; Saren, G.; Jiang, M.; Zhang, H.; Yu, X.

    Viral Nucleic Acid Kit (Roche) following the manufac- turer’s instructions (Zhang et al., 2010). Bacteria-specific primers 8F (AGRGTTTGATCCTGGCTCAG) and 1492R (CGG CTACCT- TGTTACGACTT) were used for 16S rRNA gene amplification. PCR reaction mixture (25 μ...Finder dye (Biov) and the correct bands were excised and purified using Zymoclean Gel DNA Recovery KitTM accord- ing to manufacturer’s protocol. The gel-purified 16S rRNA gene was ligated into pMD19-T simple vectors (Takara) according to the manufacturer’s...

  6. 抢眼视觉感

    Institute of Scientific and Technical Information of China (English)



    将各种棱角、切面组合出新的结构,令每一件单品在空间中的存在感都大大增强,同时为陈设带来更丰富的呈现效果。1、模拟钻石切割面的Hydra吊灯(calligaris) 2、深受caravaggio作品影响的碎片花器,由Mario Trimarchi设计。(Alessi) 3、切割面茶几(罗奇堡Roche-Bobois)

  7. D-Branes, RR-Fields and Duality on Noncommutative Manifolds

    CERN Document Server

    Brodzki, J; Rosenberg, J; Szabó, R J; Brodzki, Jacek; Mathai, Varghese; Rosenberg, Jonathan; Szabo, Richard J.


    We develop some of the ingredients needed for string theory on noncommutative spacetimes, proposing an axiomatic formulation of T-duality as well as establishing a very general formula for D-brane charges. This formula is closely related to a noncommutative Grothendieck-Riemann-Roch theorem that is proved here. Our approach relies on a very general form of Poincare duality, which is studied here in detail. Among the technical tools employed are calculations with iterated products in bivariant K-theory and cyclic theory, which are simplified using a novel diagram calculus reminiscent of Feynman diagrams.

  8. 单克隆抗体Tocilizumab获准用于治疗活动期全身型幼年特发性关节炎

    Institute of Scientific and Technical Information of China (English)


    @@ 由罗氏(Roche)旗下基因泰克(Genentech)公司开发的单克隆抗体tocilizumab(商品名为Aetemra)于近期获FDA批准,用于治疗活动期全身型幼年特发性关节炎(systemic juvenile idiopathic arthritis,SJIA),适用年龄为2岁及2岁以上,可单独使用,也可与甲氨蝶呤合用.SJIA也称斯蒂尔病(Still's dis-ease),为一种罕见的可威胁生命的疾病.

  9. Reading the Body in Bret Easton Ellis's American Psycho (1991) : Confusing Signs and Signifiers


    Roche, David

    2009-01-01; International audience; This article examines how matter, in Ellis's scandalous novel, is read according to the texts that inform the yuppie's etiquette, labeling the body as either “healthy” or “unhealthy.” Basing his argumentation on the works of Judith Butler, Luce Irigaray, Jacques Lacan, Clément Rosset and D.W. Winnicott, the author argues that the body is perceived as a sign of something when it is actually an effect of the signifier, and that the novel's ...

  10. Sustainable building with closed cavity facades. Top energy efficiency and more daylight; Nachhaltig bauen mit Closed Cavity Fassaden. Hoechste Energieeffizienz und mehr Tageslicht

    Energy Technology Data Exchange (ETDEWEB)

    Rudolf, Bernhard


    Facades have a key function within the sustainable construction since the energy conservation significantly depends on the building envelope. Beside an enhanced energy efficiency, the Green Building shall offer an improved utilization of day-lighting and comfortable indoor climatic conditions. Here, the innovative Closed Cavity Facade new standards. The former 'Poseidon building' in Frankfurt/Main (Federal Republic of Germany) and the multi-storey building of the Roche company in Basel (Switzerland) are equipped with this facade. The energy costs, operational costs as well as the maintenance costs are reduced sustainably using this closed bivalved facade.

  11. Minimizing the risk of chemically reactive metabolite formation of new drug candidates: implications for preclinical drug design. (United States)

    Brink, Andreas; Pähler, Axel; Funk, Christoph; Schuler, Franz; Schadt, Simone


    Many pharmaceutical companies aim to reduce reactive metabolite formation by chemical modification at early stages of drug discovery. A practice often applied is the detection of stable trapping products of electrophilic intermediates with nucleophilic trapping reagents to guide rational structure-based drug design. This contribution delineates this strategy to minimize the potential for reactive metabolite formation of clinical candidates during preclinical drug optimization, exemplified by the experience at Roche over the past decade. For the majority of research programs it was possible to proceed with compounds optimized for reduced covalent binding potential. Such optimized candidates are expected to have a higher likelihood of succeeding throughout the development processes, resulting in safer drugs.

  12. Rituximab可用于治疗慢性淋巴细胞白血病

    Institute of Scientific and Technical Information of China (English)


    @@ 罗氏(Roche)公司与生物基因艾迪克(Biogen Idec)公司联合开发的抗CD20药物Rituxan/MabThera(通用名:利妥昔单抗),近期已获美国FDA批准,与化疗联合用于初发或已经接受过治疗(复发性或顽固性)的慢性淋巴细胞白血病(chronic lymphocytic leukaemia,CLL)患者.

  13. Comparison of the Reference Intervals Used for the Evaluation of Maternal Thyroid Function During Pregnancy Using Sequential and Nonsequential Methods

    Institute of Scientific and Technical Information of China (English)

    Jian-Xia Fan; Shuai Yang; Wei Qian; Feng-Tao Shi; He-Feng Huang


    Background:Maternal thyroid dysfunction is common during pregnancy,and physiological changes during pregnancy can lead to the overdiagnosis of hyperthyroidism and misdiagnosis of hypothyroidism with nongestation-specific reference intervals.Our aim was to compare sequential with nonsequential methods for the evaluation of thyroid function in pregnant women.Methods:We tested pregnant women who underwent their trimester prenatal screening at our hospital from February 2011 to September 2012 for serum thyroid stimulating hormone (TSH) and free thyroxine (FT4) using the Abbott and Roche kits.There were 447 and 200 patients enrolled in the nonsequential and sequential groups,respectively.The central 95% range between the 2.5th and the 97.5th percentiles was used as the reference interval for the thyroid function parameter.Results:The nonsequential group exhibited a significantly larger degree of dispersion in the TSH reference interval during the 2nd and 3rd trimesters as measured using both the Abbott and Roche kits (all P < 0.05).The TSH reference intervals were significantly larger in the nonsequential group than in the sequential group during the 3rd trimester as measured with both the Abbott (4.95 vs.3.77 mU/L,P < 0.001) and Roche kits (6.62 vs.5.01 mU/L,P =0.004).The nonsequential group had a significantly larger FT4 reference interval as measured with the Abbott kit during all trimesters (12.64 vs.5.82 pmol/L;7.96 vs.4.77 pmol/L;8.10 vs.4.77 pmol/L,respectively,all P < 0.05),whereas a significantly larger FT4 reference interval was only observed during the 24 trimester with the Roche kit (7.76 vs.5.52 pmol/L,P =0.002).Conclusions:It was more reasonable to establish reference intervals for the evaluation of maternal thyroid function using the sequential method during each trimester of pregnancy.Moreover,the exclusion of pregnancy-related complications should be considered in the inclusion criteria for thyroid function tests.

  14. L'atténuation intrinsèque des ondes sismiques. Troisième partie : Mécanismes régissant l'atténuation. Intrinsic Attenuation of Seismic Waves. Part Three: Mechanisms Governing Attenuation

    Directory of Open Access Journals (Sweden)

    Bourbie T.


    Full Text Available Les mécanismes qui régissent l'atténuation sont très complexes et encore mal compris actuellement. Certains mécanismes ont pu être éliminés comme le phénomène de frottement grain à grain du fait des amplitudes de déformation usuelles en sismique. Il. semble que les mécanismes responsables soient différents dans le cas de roches sèches (aux conditions de laboratoire et dans le cas de roches plus ou moins saturées. Dans le cas de roches sèchesl'atténuation semble provenir à la fois d'un hystérésis microcapillaire et d'une rupture des liaisons hydrogènes entre les hydroxyles de la surface et les molécules d'eau. Dans le cas de roches saturées, les candidats les plus probables au phénomène d'atténuation sont les mécanismes de Biot et de squirt flow . Le mécanisme de Biot est la possibilité d'avoir des mouvements différentiels globaux (ou plutôt moyennés à l'échelle de l'échantillon entre la matière solide et le fluide. Le squirt flowconsiste en un phénomène de relaxation visqueuse dont l'excitateur est extérieur : le liquide à l'intérieur de la cavité poreuse jaillit sous l'effet d'une pression de pore locale. The mechanisms governing attenuation are highly complex and still poorly understood at present. Some mechanisms have been eliminated, such as the grain-to-grain friction, due to the usual deformation amplitudes in seismic exploration. The mechanisms responsible for attenuation seem to be different for dry rocks (under laboratory conditions and more or less saturated rocks. For dryrocks, attenuation seems to come from both a microcapillary hysteresis and a break of the hydrogen bonds between the surface hydroxyls and water molecules. For saturated rocks, the most probable causes of attenuation are Biot mechanisms and squirt flow. The Biot mechanism Is the possibility of having overall differential movements (or rather averaged ones on the scale of the sample between the solid matrix and the fluid

  15. Analysis of conditional gene deletion using probe based Real-Time PCR

    Directory of Open Access Journals (Sweden)

    Lyko Frank


    Full Text Available Abstract Following publication of this article 1 the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science". The correct probe that was used for the 1lox/2lox allele ratio analysis in the paper is as follows Probe for 1lox/2lox allele quantification: 5'-6-FAM-atAaCtTCgtatagCATaCattatac-BHQ-1 -3' (uppercase letters = LNA bases Manufacturer: EUROGENTEC, Seraing, Belgium All other information and reaction conditions in the paper are correct as stated.

  16. Active and realistic passive marijuana exposure tested by three immunoassays and GC/MS in urine

    Energy Technology Data Exchange (ETDEWEB)

    Mule, S.J.; Lomax, P.; Gross, S.J.


    Human urine samples obtained before and after active and passive exposure to marijuana were analyzed by immune kits (Roche, Amersham, and Syva) and gas chromatography/mass spectrometry (GC/MS). Seven of eight subjects were positive for the entire five-day test period with one immune kit. The latter correlated with GC/MS in 98% of the samples. Passive inhalation experiments under conditions likely to reflect realistic exposure resulted consistently in less than 10 ng/mL of cannabinoids. The 10-100-ng/mL cannabinoid concentration range essential for detection of occasional and moderate marijuana users is thus unaffected by realistic passive inhalation.

  17. Rotational mixing in close binaries

    CERN Document Server

    de Mink, S E; Langer, N; Yoon, S -Ch; Brott, I; Glebbeek, E; Verkoulen, M; Pols, O R


    Rotational mixing is a very important but uncertain process in the evolution of massive stars. We propose to use close binaries to test its efficiency. Based on rotating single stellar models we predict nitrogen surface enhancements for tidally locked binaries. Furthermore we demonstrate the possibility of a new evolutionary scenario for very massive (M > 40 solar mass) close (P < 3 days) binaries: Case M, in which mixing is so efficient that the stars evolve quasi-chemically homogeneously, stay compact and avoid any Roche-lobe overflow, leading to very close (double) WR binaries.

  18. [Contribution of the laboratory in hypoglycemia diagnosis induced by insulin administration in a 2-year-old girl]. (United States)

    Dejoie, T; Ramos, E; Baron, S; Bach-Ngohou, K; Masson, D


    We report the case of a 2 year-old child presented to the emergency department following a seizure. The child was hypotonic and examination was unremarkable but laboratory tests confirmed a severe hypoglycaemia. The insulin level, inappropriately high for the glycemia and the peptide C undetectable suggested exogenous hyperinsulinism. We conclude that the hypoglycaemia was likely the result of Munchhausen syndrome by proxy. The specificity of two immunoassays used (Elecsys Roche and IRMA CisBio) for the synthetic analogues of insulin explains the discrepancy between the insulin levels obtained but was crucially useful to the approach of the cause of the hypoglycaemia.

  19. Star Formation Close to Sgr A* and Beyond the Nuclear Cluster

    CERN Document Server

    Yusef-Zadeh, F


    Two modes of star formation are involved to explain the origin of young stars near Sgr A*. One is a disk-based mode, which explains the disk of stars orbiting Sgr A*. The other is the standard cloud-based mode observed in the Galactic disk. We discuss each of these modes of star formation and apply these ideas to the inner few parsecs of Sgr A*. In particular, we focus on the latter mode in more detail. We also discuss how the tidal force exerted by the nuclear cluster makes the Roche density approaching zero and contributes to the collapse of molecular clouds located tens of parsecs away from Sgr A*.

  20. Argiles et zéolites dans l'altération d'un volcan bouclier en milieu tropical (Le Piton des Neiges, La Réunion)Clays and zeolites in the weathering of a basaltic tropical shield volcano ('Le Piton des Neiges', Reunion Island) (United States)

    Bret, Laurent; Join, Jean-Lambert; Legal, Xavier; Coudray, Jean; Fritz, Bertrand


    The drilling of a gallery through the Roche-Écrite massif (Reunion Island) allowed to sample the old formations of the 'Piton des Neiges' volcano. The mineralogical study of the alteration products of the formations showed significant variations of the zeolites/clay minerals ratio depending on the location of the rocks in the volcano. The abundance of zeolites in the formation near the surface hydrographic network or the underground water drainage system seems to be due to the physicochemical processes favouring zeolites in more open systems in their competition with secondary clays. To cite this article: L. Bret et al., C. R. Geoscience 335 (2003).

  1. Development of Targeted Nonionic Surfactant Vesicles for Treatment of Vascular Injury (United States)


    cardiac puncture. Plasma was analyzed for total cholesterol, HDL cholesterol, LDL cholesterol and triglycerides on a Cobas Mira Plus analyzer (Yale...Phenotyping Center, Yale University School of Medicine, New Haven, CT LDL -direct reagents purchased from Roche; triglycerides, total cholesterol, HDL ...787.6 ± 337.7 832.1 ± 517.3 701.4 ± 276.2 N 4 5 6 5 6 5 5 HDL -direct (mg/dl) 15.8 ± 6.1 11.4 ± 4.1 12.4 ± 3.4 11.7 ± 3.5 10.6 ± 4.1

  2. Workshop Introduction - Building Energy Performance Improvement Through Advanced Technologies, Smart Organization, and Financing (United States)


    A&M University, ESL Continuous Commissioning—An Effective Method for Energy Reductions Ch 5 14:00-14:15 Mr. Michael Chimack, University of Illinois...PNNL Energy assessment and analysis using FEDS Ch 7 8:45-9:00 Dr. Michael Witte, GardAnalytics Whole building energy analysis using EnergyPlus 9:00-9...Assessment of HVAC systems reliability 14:30-15:00 Coffee break Ch 8 15:00-15:15 Mr. Roch Ducey , ERDC-CERL Energy Security 15:15-15:30 Mr

  3. Advances in astronomy and astrophysics 9

    CERN Document Server

    Kopal, Zdenek


    Advances in Astronomy and Astrophysics, Volume 9 covers reviews on the advances in astronomy and astrophysics. The book presents reviews on the Roche model and its applications to close binary systems. The text then describes the part played by lunar eclipses in the evolution of astronomy; the classical theory of lunar eclipses; deviations from geometrical theory; and the methods of photometric observations of eclipses. The problems of other phenomena related in one way or another to lunar eclipses are also considered. The book further tackles the infrared observation on the eclipsed moon, as

  4. Violencia de los usuarios hacia el personal de enfermería : evaluación del riesgo laboral y análisis de las consecuencias psicológicas


    Galián Muñoz, Inmaculada


    La presente tesis doctoral se centra en el estudio de la exposición a la violencia de los usuarios del personal de enfermería de los hospitales públicos de la Región de Murcia, ya que el riesgo de violencia laboral presenta una especial importancia en el ámbito sanitario, siendo el colectivo de enfermería uno de los más afectados tal y como se refleja en distintos estudios (Franz, Zeh, Schablon, Kuhnert y Nienhaus, 2010; Kling, Yassi, Smailes, Lovato y Koehoorn, 2009; Roche, Diers, Duffield y...

  5. Searches for Optical Counterparts to Fermi Unassociated Sources with the Intermediate Palomar Transient Factory (United States)

    Bellm, Eric Christopher; Prince, Thomas A.; Kaplan, David L. A.; Kupfer, Thomas; DeCesar, Megan E.; Laher, Russ; Masci, Frank J.; Shupe, David L.; Intermediate Palomar Transient Factory Collaboration


    The Intermediate Palomar Transient Factory (iPTF) has accumulated an extensive optical variability dataset across the Northern Sky, including at low Galactic latitudes (|b| < 20 degrees). We are using this dataset to search for optical counterparts to unassociated Fermi gamma-ray sources, particular the companions of eclipsing binary millisecond pulsars. So-called redback binary millisecond pulsars are a key evolutionary stage in the recycling process that spins up millisecond pulsars. The Roche-distorted and irradiated pulsar companion produces a periodic signature at the orbital period that may be readily identified with iPTF. We report on the progress of this search and present interesting candidates found.

  6. Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing

    DEFF Research Database (Denmark)

    Bohmann, Kristine; Monadjem, Ara; Noer, Christina Lehmkuhl


    Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep...... sequences in the GenBank and BOLD databases, similarity to existing collections allowed the preliminary identification of 25 prey families from six orders of insects within the diet of C. pumilus, and 24 families from seven orders within the diet of M. condylurus. Insects identified to families within...

  7. Are adults with bipolar disorder active? Objectively measured physical activity and sedentary behavior using accelerometry


    Janney, Carol A; Fagiolini, Andrea; Swartz, Holly A.; Jakicic, John M.; Holleman, Robert G.; Richardson, Caroline R


    Carol A Janney has received research support from Actigraph, Inc for this study. Andrea Fagiolini is/has been a consultant and/or speaker and/or has received grants from: Angelini, Astra Zeneca, Bristol-Myers Squibb, Boehringer Ingelheim, Pfizer, Eli Lilly, Janssen, Lundbeck, Novartis, Otsuka, Roche and Sigma Tau. Holly A. Swartz has received honoraria from Servier, Astra Zeneca, SciMed, Bristol-Myers Squibb, Eli Lilly, and Sanofi. She has received royalties from UpToDate. John M. Ja...

  8. Maximum mass ratio of AM CVn-type binary systems and maximum white dwarf mass in ultra-compact X-ray binaries

    Directory of Open Access Journals (Sweden)

    Arbutina Bojan


    Full Text Available AM CVn-type stars and ultra-compact X-ray binaries are extremely interesting semi-detached close binary systems in which the Roche lobe filling component is a white dwarf transferring mass to another white dwarf, neutron star or a black hole. Earlier theoretical considerations show that there is a maximum mass ratio of AM CVn-type binary systems (qmax ≈ 2/3 below which the mass transfer is stable. In this paper we derive slightly different value for qmax and more interestingly, by applying the same procedure, we find the maximum expected white dwarf mass in ultra-compact X-ray binaries.

  9. Advanced Methods for Passive Acoustic Detection, Classification, and Localization of Marine Mammals (United States)


    Kershenbaum, A., and Roch, M. A. (2013). "An image processing based paradigm for the extraction of tonal sounds in cetacean communications," J. Acous. Soc...Press, Cambridge. Martin, A.Q., Marques , T.A., Thomas, L., Morrissey, R.P., Jarvis, S., DiMarzio, SN., and Moretti, D. 2013. Estimating minke...Society of America 129:4055-4061. Martin, S.W., T.A. Marques , L. Thomas, R.P. Morrissey, S. Jarvis, N. DiMarzio, D. Moretti, and D.K. Mellinger. 2012

  10. Determination of renal clearances of amylase/creatinine with chromogenic and enzymatic methods. (United States)

    Hohenwallner, W; Wimmer, E; Sommer, R


    Urinary amylase was estimated by chromogenic (amylochrome Roche) as well as enzymatic methods (SKI and Beckman: substrate starch and substrate maltotetraose respectively). Random and timed urines (24 hour collections) were analysed. Clearances of amylase gave different results dependent upon the amylase-test used and the glomerular filtration rate. Correlation between chromogenic and enzymatic methods (starch as substrate) was poor. The ratio of amylase and creatinine clearance was used to test different methods. Reference values for this ratio for the amylochrome method (N = 106) were 2.85 +/- 0.99% and for the Beckman-DS method (N = 60) 2.82 +/- 0.87%.

  11. Computing a Function of Correlated Sources

    CERN Document Server

    Sefidgaran, Milad


    A receiver wants to compute a function f of two correlated sources X and Y and side information Z. What is the minimum number of bits that needs to be communicated by each transmitter? In this paper, we derive inner and outer bounds to the rate region of this problem which coincide in the cases where f is partially invertible and where the sources are independent given the side information. From the former case we recover the Slepian-Wolf rate region and from the latter case we recover Orlitsky and Roche's single source result.


    Energy Technology Data Exchange (ETDEWEB)

    Matranga, Marco; Drake, Jeremy J.; Kashyap, Vinay [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States); Steeghs, Danny, E-mail: [Department of Physics, University of Warwick, Coventry CV4 7AL (United Kingdom)


    An XMM-Newton observation of the nearby 'pre-cataclysmic' short-period (P{sub orb} = 3.62 hr) binary QS Vir (EC 13471-1258) revealed regular narrow X-ray eclipses when the white dwarf passed behind its M2-4 dwarf companion. The X-ray emission provides a clear signature of mass transfer and accretion onto the white dwarf. The low-resolution XMM-Newton EPIC spectra are consistent with a cooling flow model and indicate an accretion rate of M-dot = 1.7 Multiplication-Sign 10{sup -13} M{sub sun} yr{sup -1}. At 48 pc distant, QS Vir is then the second nearest accreting cataclysmic variable known, with one of the lowest accretion rates found to date for a non-magnetic system. To feed this accretion through a wind would require a wind mass-loss rate of M-dot {approx}2 Multiplication-Sign 10{sup -12} M{sub sun} yr{sup -1} if the accretion efficiency is of the order of 10%. Consideration of likely mass-loss rates for M dwarfs suggests this is improbably high and pure wind accretion unlikely. A lack of accretion disk signatures also presents some difficulties for direct Roche lobe overflow. We speculate that QS Vir is on the verge of Roche lobe overflow, and that the observed mass transfer could be supplemented by upward chromospheric flows on the M dwarf, analogous to spicules and mottles on the Sun, that escape the Roche surface to be subsequently swept up into the white dwarf Roche lobe. If so, QS Vir would be in a rare evolutionary phase lasting only a million years. The X-ray luminosity of the M dwarf estimated during primary eclipse is L{sub X} = 3 Multiplication-Sign 10{sup 28} erg s{sup -1}, which is consistent with that of rapidly rotating 'saturated' K and M dwarfs.

  13. Novel Therapeutic Targets to Inhibit Tumor Microenvironment-Induced Castration-Resistant Prostate Cancer (United States)


    signaling in HPS19I cells, we transfected HPS19I cells with a (CAGA)12MLP (Smad binding sequence) reporter construct [41] and a pRL -null vector...transfected with (CAGA)12MLP and pRL -null vectors, and treated with 50 pM of TGF-β1 and different dosages of SD-208 compound for 24 hours. Cell lysates were...CAGA)12MLP [41] and 0.1 μg of pRL -null (Promega, Madison, WI, USA) in 12 well plate using FuGENE 6 transfection reagent (Roche Applied Sciences

  14. ING4 Loss in Prostate Cancer Progression (United States)


    transcription system (Promega). Synthesized cDNA was amplified for qRT-PCR using SYBR Green Master Mix (Roche) with gene-specific primers and an ABI 7500 RT...siMyc) or a scrambled sequence ( Scr ) on days 2, 4, and 6. D, ING4 andMyc expressionwere detected in undifferentiated (iPr) or in siRNA- treated...Myc or ING4 overexpressing cells were transfected with Bnip3 siRNA (siBnip3) or a scrambled sequence ( Scr ) and immunostained 72 hours later for

  15. Numerical calculations of mass transfer flow in semi-detached binary systems. [of stars (United States)

    Edwards, D. A.; Pringle, J. E.


    The details of the mass transfer flow near the inner Lagrangian point in a semidetached binary system are numerically calculated. A polytropic equation of state with n = 3/2 is used. The dependence of the mass transfer rate on the degree to which the star overfills its Roche lobe is calculated, and good agreement with previous analytic estimates is found. The variation of mass transfer rate which occurs if the binary system has a small eccentricity is calculated and is used to cast doubt on the model for superhumps in dwarf novae proposed by Papaloizou and Pringle (1979).

  16. Influence of Asymptomatic Pneumonia on the Response to Hemorrhage and Resuscitation in Swine (United States)


    arterial and venous blood gases, pH and base excess (AVL Omni 9 Analyzer, Roche Diagnostics, Indianapolis, IN); 2) hematocrit (Hct), hemo- globin ( Hb ...included the following: Arterial O2 content (ArO2) = (SaO2 * 1.34 * Hb * .01) + (PaO2 * .003) = ml/dl Venous O2 content (VeO2) = (SvO2 * 1.34 * Hb ...pneumonia swine. In addition, plasma levels of IL-1b and IL-10 cytokines were determined using swine immunoassay kits (Catalog # KSC0012/KSC0011 and

  17. Hemoglobin variants detected by hemoglobin A1c (HbA1c) analysis and the effects on HbA1c measurements


    Nasir, Nadzimah Mohd; Thevarajah, M; Yean, Chew Yee


    Background: Hemoglobin (Hb) A1c is a tool widely used to monitor long-term glycemic control in diabetic patients. The objective of our study is to compare the HbA1c values measured on high performance liquid chromatography (HPLC) and immunoassay in patients who were detected to have hemoglobin variant after HbA1c analysis. Materials and Methods: We compared the HbA1c values measured using the Arkray Adams A1c HA-8160 (HPLC method) and Roche Cobas Integra (immunoturbidimetric method) from diab...

  18. Reference intervals for C-peptide and insulin derived from a general adult Danish population

    DEFF Research Database (Denmark)

    Larsen, Pia Bükmann; Linneberg, Allan; Hansen, Torben


    BACKGROUND: Despite international efforts to standardize C-peptide and insulin calibrators and immunoassays, platform dependent differences still exist, and platform specific reference intervals are hence needed for correct interpretation. We therefore wanted to establish traceable reference...... intervals for C-peptide and insulin. METHODS: In 623 consecutively recruited participants, insulin and C-peptide were measured using the Cobas e411 (Roche Diagnostics, Switzerland). Participants with diabetes were excluded (fasting Glucose ≥7.0mmol/L or HbA1c≥6.5%/≥48mmol/L) and reference intervals were...


    DEFF Research Database (Denmark)

    Tørring, Niels; Aulesa, C; Eiben, Bernd;


    Background Screening for fetal trisomy 21 (T21) in the first trimester includes analysis of the serological markers pregnancy-associated plasma protein A (PAPP-A) and free beta choriogonadotropin (free βhCG). With the launch of these assays on the cobas e and Elecsys platforms, we investigated...... their clinical and analytical performance. Patients and Methods We conducted a multicenter study in 5397 pregnancies including 108 cases with verified fetal T21 at 8 to 14 weeks of gestation. A technical validation of the Roche Elecsys® free βhCG and PAPP-A assays were performed, including method comparisons...

  20. Private visit to the CMS assembly site of Dr. Fidel Castro Diaz-Balart from the Superior Institute of Sciences and Nuclear Technologies, Havana, accompanied by His Excellency Mr. Emilio Caballero, Ambassador, Permanent Mission of Cuba in Paris.

    CERN Document Server

    Maximilien Brice


    Photo 01: Left to right: His Excellency Mr Emilio Caballero; Prof. Tejinder Virdee, Deputy Spokesman of the CMS experiment; Dr Fidel Castro Diaz-Balart; Dr Matthias Schroeder, physicist, Experimental Physics division; Mrs Noëlle Levy, Casa del Habano, Geneva; Dr John Ellis, Adviser for Non-Member State relations; Dr Christian Roche, Senior Advisor to the Director-General. Photo 02: Left to right: His Excellency Mr Emilio Caballero, Prof. Tejinder Virdee, Dr Fidel Castro Diaz-Balart; Dr Matthias Schroeder; Mrs Noëlle Levy, Prof. Juan Antonio Rubio, Head of the Education and Technology Transfer division; Dr John Ellis.

  1. Foreign-branded Furniture Amid Crisis of Confidence

    Institute of Scientific and Technical Information of China (English)

    Zhu Zijun


    @@ "The clients raised questions about the place of origin of our furniture, and almost all the new orders have been cancelled or postponed since the Da Vinci controversy was reported," said Zhou Yufeng, China managing directorof the Roche Bobois (RB) Group, at a press conference held on July 20in Shanghai. His words showed his concerns of RB in China.However, RB isnot the only furniture firm to suffer from the Da Vinci scandal.Otherforeign brands are also facing a credibility crisis.

  2. Interacting binary stars

    CERN Document Server

    Sahade, Jorge; Ter Haar, D


    Interacting Binary Stars deals with the development, ideas, and problems in the study of interacting binary stars. The book consolidates the information that is scattered over many publications and papers and gives an account of important discoveries with relevant historical background. Chapters are devoted to the presentation and discussion of the different facets of the field, such as historical account of the development in the field of study of binary stars; the Roche equipotential surfaces; methods and techniques in space astronomy; and enumeration of binary star systems that are studied

  3. Diversidade e estrutura da comunidade bacteriana associada às armadilhas da planta carnívora Utricularia gibba (Lentibulariaceae) e ao ambiente aquático.


    Almir José Ferreira


    A diversidade microbiana em ambientes aquáticos e sua associação com plantas carnívoras ainda é pouco estudada. Assim, a comunidade bacteriana da planta carnívora Utricularia gibba e do seu meio aquático foi avaliada por meio do seqüenciamento em larga escala (454 Roche) de uma biblioteca do gene 16S rRNA. Os resultados indicaram que a comunidade bacteriana na água é significativamente diferente da comunidade dos utrículos. Além disso, a comunidade bacteriana da água é composta principalmente...

  4. The first light-curve analysis of eclipsing binaries observed by the INTEGRAL/OMC

    CERN Document Server

    Zasche, P


    Three Algol-type binaries in Cygnus constellation were selected for an analysis from a huge database of observations made by the INTEGRAL/OMC camera. These data were processed and analyzed, resulting in a first light-curve study of these neglected eclipsing binaries. The temperatures of the primary components range from 9500 K to 10500 K and the inclinations are circa 73deg (for PV Cyg and V1011 Cyg), while almost 90deg for V822 Cyg. All of them seem to be main-sequence stars, well within their critical Roche lobes. Nevertheless, further detailed analyses are still needed.

  5. Ultra-stripped supernovae and double neutron star systems

    CERN Document Server

    Tauris, Thomas


    The evolution of close-orbit progenitor binaries of double neutron star (DNS) systems leads to supernova (SN) explosions of ultra-stripped stars. The amount of SN ejecta mass is very limited from such, more or less, naked metal cores with envelope masses of only 0.01-0.2 Msun. The combination of little SN ejecta mass and the associated possibility of small NS kicks is quite important for the characteristics of the resulting DNS systems left behind. Here, we discuss theoretical predictions for DNS systems, based on Case BB Roche-lobe overflow prior to ultra-stripped SNe, and briefly compare with observations.

  6. Evolutionary Processes in Multiple Systems

    Energy Technology Data Exchange (ETDEWEB)

    Eggleton, P P; Kisseleva-Eggleton, L


    There are several ways in which triple stars can evolve in somewhat unusual ways. They discuss two situations where Case A Roche-lobe overflow, followed by a merger, can produce anomalous wide binaries such as {gamma} Per; and Kozai cycles in triples with non-parallel orbits, which can produce merged rapidly-rotating stars like AB Dor, and which can also lead to the delayed ejection of one component of a multiple, as may have been observed in T Tau in 1998.

  7. The genome of the fire ant Solenopsis invicta

    DEFF Research Database (Denmark)

    Wurm, Yannick; Wang, John; Riba-Grognuz, Oksana;


    Ants have evolved very complex societies and are key ecosystem members. Some ants, such as the fire ant Solenopsis invicta, are also major pests. Here, we present a draft genome of S. invicta, assembled from Roche 454 and Illumina sequencing reads obtained from a focal haploid male and his brothers...... 400 putative olfactory receptors of which at least 297 are intact. This represents the largest repertoire reported so far in insects. S. invicta also harbors an expansion of a specific family of lipid-processing genes, two putative orthologs to the transformer/feminizer sex differentiation gene...

  8. Molecular diet analysis of two African free-tailed bats (Molossidae) using high throughput sequencing

    DEFF Research Database (Denmark)

    Bohmann, Kristine; Monadjem, Ara; Noer, Christina Lehmkuhl;


    Given the diversity of prey consumed by insectivorous bats, it is difficult to discern the composition of their diet using morphological or conventional PCR-based analyses of their faeces. We demonstrate the use of a powerful alternate tool, the use of the Roche FLX sequencing platform to deep......-sequence uniquely 5′ tagged insect-generic barcode cytochrome c oxidase I (COI) fragments, that were PCR amplified from faecal pellets of two free-tailed bat species Chaerephon pumilus and Mops condylurus (family: Molossidae). Although the analyses were challenged by the paucity of southern African insect COI...

  9. Role of RNA Processing Factors in the Expression of Flt-1 and its Secreted Variant, sFlt-1


    Roche, Rebecca I.


    Role of RNA Processing Factors in the Expression of Flt-1 and its Secreted Variant, sFlt-1 By Rebecca I. Roche, MS Dr. W.R. Huckle, Chairman Department of Biomedical Sciences and Pathobiology (ABSTRACT) Vascular endothelial growth factor (VEGF) is an endothelial cell mitogen involved in angiogenesis, the formation of new blood vessels. sFlt-1, a secreted form of the signal-transducing VEGF receptor Flt-1, can inhibit cellular responses to VEGF both in vitro and in viv...

  10. Temporal Control of Transforming Growth Factor (TGF) - Betal Expression on Mammary Cell Multistep Transformation (United States)


    heratopoetic origin and epithelial lin- ml of Vindelov’s PI buffer pH 8.0 (10 mM Trizma base, 10 mM cage. 3 TGF-3 inhibits the growth of normal epithelial...plasmid construct containing cDNA encoding a truncated systems; Foster City, CA) in l X PCR buffer . Cycling parameters TGF-[3RII lacking the serine...with ice-cold PBS and scraped into a buffer containing 50 mM triphosphate RNA-labeling mix (number 1685 597; Roche Molec- Tris-HCl, pH 7.4, 150 mM NaCI

  11. Ultraluminous X-ray Sources as Intermediate Mass Black Holes Fed by Tidally Captured Stars

    CERN Document Server

    Hopman, C; Alexander, T; Hopman, Clovis; Zwart, Simon F. Portegies; Alexander, Tal


    The nature of ultraluminous X-ray sources (ULXs) is presently unknown. A possible explanation is that they are accreting intermediate mass black holes (IBHs) that are fed by Roche lobe overflow from a tidally captured stellar companion. We show that a star can circularize around an IBH without being destroyed by tidal heating (in contrast to the case of M_bh> 10^6 M_sun massive black holes in galactic centers, where survival is unlikely). We find that the capture and circularization rate is of the order of 5 \\times 10^-8 yr^-1, almost independently of the cluster's relaxation time. We follow the luminosity evolution of the binary system during the main sequence Roche lobe overflow phase and show it can maintain ULX-like luminosities for >10 Myr. In particular, we show that the ULX in the young cluster MGG-11 in star-burst galaxy M82, which possibly harbors an IBH, is well explained by this mechanism, and we predict that \\gtrsim 10% of similar clusters with IBHs have a tidally captured circularized star. The c...

  12. Formation of diverse ring-satellite systems around Centaurs through tidal disruption at close encounters with giant planet

    CERN Document Server

    Hyodo, Ryuki; Genda, Hidenori; Ohtsuki, Keiji


    Centaurs are minor planets orbiting between Jupiter and Neptune that have or had crossing orbits with one or more giant planets. Recent observations and reinterpretation of previous observations have revealed the existence of ring systems around 10199 Chariklo and 2060 Chiron. However, the origin of the ring systems around such a minor planet is still an open question. Here, we propose that the tidal disruption of a differentiated object that experiences a close encounter with a giant planet could naturally form diverse ring-satellite systems around the Centaurs. During the close encounter, the icy mantle of the passing object is preferentially ripped off by the planet's tidal force and the debris is distributed mostly within the Roche limit of the largest remnant body. Assuming the existence of $20-50$wt% silicate core below the icy mantle, a disk of particles is formed when the objects pass within $0.4-0.8$ of the planet's Roche limit with the relative velocity at infinity $3-6$km s$^{-1}$ and 8h initial sp...

  13. A Wind Accretion Model for HLX-1

    CERN Document Server

    Miller, M Coleman; Maccarone, Thomas J


    The brightest ultraluminous X-ray source currently known, HLX-1, has been observed to undergo five outburst cycles. The periodicity of these outbursts, and their high inferred maximum accretion rates of $\\sim{\\rm few}\\times 10^{-4} M_\\odot {\\rm yr}^{-1}$, naturally suggest Roche lobe overflow at the pericenter of an eccentric orbit. It is, however, difficult for the Roche lobe overflow model to explain the apparent trend of decreasing decay times over the different outbursts while the integrated luminosity also drops. Thus if the trend is real rather than simply being a reflection of the complex physics of accretion disks, a different scenario may be necessary. We present a speculative model in which, within the last decade, a high-mass giant star had most of its envelope tidally stripped by the $\\sim 10^{4-5} M_\\odot$ black hole in HLX-1, and the remaining core plus low-mass hydrogen envelope now feeds the hole with a strong wind. This model can explain the short decay time of the disk, and could explain the...

  14. Understanding the evolution of close binary systems with radio pulsars

    CERN Document Server

    Benvenuto, O G; Horvath, J E


    We calculate the evolution of close binary systems (CBSs) formed by a neutron star (behaving as a radio pulsar) and a normal donor star, evolving either to helium white dwarf (HeWD) or ultra short orbital period systems. We consider X-ray irradiation feedback and evaporation due to radio pulsar irradiation. We show that irradiation feedback leads to cyclic mass transfer episodes, allowing CBSs to be observed in-between as binary radio pulsars under conditions in which standard, non-irradiated models predict the occurrence of a low mass X-ray binary. This behavior accounts for the existence of a family of eclipsing binary systems known as redbacks. We predict that redback companions should almost fill their Roche lobe, as observed in PSR J1723-2837. This state is also possible for systems evolving with larger orbital periods. Therefore, binary radio pulsars with companion star masses usually interpreted as larger than expected to produce HeWDs may also result in such {\\it quasi - Roche Lobe Overflow} states, r...

  15. IROme, a New High-Throughput Molecular Tool for the Diagnosis of Inherited Retinal Dystrophies

    Directory of Open Access Journals (Sweden)

    Daniel F. Schorderet


    Full Text Available The molecular diagnosis of retinal dystrophies is difficult because of the very important number of genes implicated and is rarely helped by genotype-phenotype correlations. This prompted us to develop IROme, a custom designed in solution-based targeted exon capture assay (SeqCap EZ Choice library, Roche NimbleGen for 60 retinitis pigmentosa-linked genes and three candidate genes (942 exons. Pyrosequencing was performed on a Roche 454 GS Junior benchtop high-throughput sequencing platform. In total, 23 patients affected by retinitis pigmentosa were analyzed. Per patient, 39.6 Mb were generated, and 1111 sequence variants were detected on average, at a median coverage of 17-fold. After data filtering and sequence variant prioritization, disease-causing mutations were identified in ABCA4, CNGB1, GUCY2D, PROM1, PRPF8, PRPF31, PRPH2, RHO, RP2, and TULP1 for twelve patients (55%, ten mutations having never been reported previously. Potential mutations were identified in 5 additional patients, and in only 6 patients no molecular diagnosis could be established (26%. In conclusion, targeted exon capture and next-generation sequencing are a valuable and efficient approach to identify disease-causing sequence variants in retinal dystrophies.

  16. Performance evaluation of new automated hepatitis B viral markers in the clinical laboratory: two quantitative hepatitis B surface antigen assays and an HBV core-related antigen assay. (United States)

    Park, Yongjung; Hong, Duck Jin; Shin, Saeam; Cho, Yonggeun; Kim, Hyon-Suk


    We evaluated quantitative hepatitis B surface antigen (qHBsAg) assays and a hepatitis B virus (HBV) core-related antigen (HBcrAg) assay. A total of 529 serum samples from patients with hepatitis B were tested. HBsAg levels were determined by using the Elecsys (Roche Diagnostics, Indianapolis, IN) and Architect (Abbott Laboratories, Abbott Park, IL) qHBsAg assays. HBcrAg was measured by using Lumipulse HBcrAg assay (Fujirebio, Tokyo, Japan). Serum aminotransferases and HBV DNA were respectively quantified by using the Hitachi 7600 analyzer (Hitachi High-Technologies, Tokyo, Japan) and the Cobas AmpliPrep/Cobas TaqMan test (Roche). Precision of the qHBsAg and HBcrAg assays was assessed, and linearity of the qHBsAg assays was verified. All assays showed good precision performance with coefficients of variation between 4.5% and 5.3% except for some levels. Both qHBsAg assays showed linearity from 0.1 to 12,000.0 IU/mL and correlated well (r = 0.9934). HBsAg levels correlated with HBV DNA (r = 0.3373) and with HBcrAg (r = 0.5164), and HBcrAg also correlated with HBV DNA (r = 0.5198; P HBcrAg assays.

  17. Erziehungstheorie, Literatur und Geschlecht Educational Theory, Literature, and Gender

    Directory of Open Access Journals (Sweden)

    Barbara Gribnitz


    Full Text Available Die Verfasserin stellt sich die Aufgabe, das „erkenntnistheoretische Potential des literarischen Textes als Quelle historisch-pädagogischer Forschung“ (S. 12 am Beispiel des Genre Bildungsroman herauszuarbeiten. Anhand zweier Romane, die weibliche Lebensläufe beschreiben (Sophie von La Roche: Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal, erweitert sie die Bildungsromandebatte in der Erziehungs- und Literaturwissenschaft um die Kategorie Geschlecht und bestimmt „den literarischen Text als privilegierten Ort dieser Auseinandersetzung“ (S. 40. Die Studie leidet unter Redundanz und oberflächlicher Lektüre der Texte.The author takes on the task of identifying the “epistemological potential of the literary text as source of historical-pedagogical research,” using the genre of the bildungsroman (12. Focusing on two novels that describe the course of female life histories (Sophie von La Roch: History of Lady Sophia Sternheim/Die Geschichte des Fräuleins von Sternheim; Friederike Helene Unger: Julchen Grünthal, she expands the debate on the bildungsroman in education and literary studies to include the categories of gender and identifies “the literary text as the privileged space for this conflict” (40. The study suffers under redundancy and superficial readings of the texts.

  18. Core radii and common-envelope evolution

    CERN Document Server

    Hall, Philip D


    Many classes of objects and events are thought to form in binary star systems after a phase in which a core and companion spiral to smaller separation inside a common envelope (CE).Such a phase can end with the merging of the two stars or with the ejection of the envelope to leave a surviving binary system.The outcome is usually predicted by calculating the separation to which the stars must spiral to eject the envelope, assuming that the ratio of the core--envelope binding energy to the change in orbital energy is equal to a constant efficiency factor $\\alpha$. If either object would overfill its Roche lobe at this end-of-CE separation, then the stars are assumed to merge. It is unclear what critical radius should be compared to the end-of-CE Roche lobe for stars which have developed cores before the start of a CE phase. After improving the core radius formulae in the widely used BSE rapid evolution code, we compare the properties of populations in which the critical radius is chosen to be the pre-CE core ra...

  19. Formation of the Double Neutron Star System PSR J1930$-$1852

    CERN Document Server

    Shao, Yong


    The spin period (185 ms) and period derivative ($1.8\\times10^{-17}\\,\\rm s\\,s^{-1}$) of the double neutron star (DNS) system PSR J1930$-$1852 recently discovered indicate that the pulsar was mildly recycled through the process of Roche-lobe overflow. This system has the longest orbital period (45 days) of the known DNS systems, and can be formed from a helium star-NS binary if the initial mass of the helium star was $ \\lesssim 4.0M_{\\odot} $; otherwise the helium star would never fill its Roche-lobe \\citep{t15}. At the moment of the supernova explosion, the mass of the helium star was $ \\lesssim3.0M_{\\odot} $. We find that the probability distribution of the velocity kick imparted to the new-born neutron star has a maximum at about $30 \\,\\rm km\\,s^{-1}$ (and a tail up to $ 260 \\,\\rm km\\,s^{-1}$), indicating that this NS most probably received a low kick velocity at birth.

  20. [Isolation and characterization of 27 polymorphic microsatellite markers in Armillaria luteo-virens ( Physalacriaceae)]. (United States)

    Xing, Rui; Gao, Qingbo; Zhang, Faqi; Li, Yinhu; Ful, Pengcheng; Zhang, Jinhua; Wang, Jiuli; Chen, Shilong


    [OBJECTIVE] The objectives of this study were to use Roche 454 GS FLX system to develop SSR markers for Armillaria luteo-virens. These datasets will be valuable for detecting genetic diversity and population structure of this species. [METHODS] We collected Armillaria luteo-virens samples from Yushu in Qinghai province, China. Total RNA was isolated by using the TRIzol reagent, after that we constructed cDNA library and performed one quarter plate of the whole run 454 pyrosequencing. We selected 98 primer pairs randomly from the 321 SSRs to evaluate their application and the polymorphism across 66 individuals (Armillaria luteo-virens) representing 3 wild populations. [RESULTS] Roche 454 sequencing yielded 197,121 reads with a total nucleotide size of 88,585,965bp. 27 of 98 SSRs loci were polymorphic. Numbers of alleles (Na) ranged from 2 to 8. Expected heterozygosity (HE) ranged from less than 0. 001 to 0. 810 at locus ALV65, while observed heterozygosity (Ho) from 0 at loci AIV64 and AIV92 to 0. 900 at loci ALV8. We found no evidence of linkage disequiliburium, however 10 of 27 SSR markers showed significant deviation from Hardy-weinberg equilibrium. [CONCLUSION] These remaining 17 pairs of Armillaria luteo-virens SSR markers will be valuable for future research on detecting population structure and conservation of this species.

  1. Coupled Evolutions of the Stellar Obliquity, Orbital Distance, and Planet's Radius due to the Ohmic Dissipation Induced in a Diamagnetic Hot Jupiter around a Magnetic T Tauri Star (United States)

    Chang, Yu-Ling; Bodenheimer, Peter H.; Gu, Pin-Gao


    We revisit the calculation of the ohmic dissipation in a hot Jupiter presented by Laine et al. by considering more realistic interior structures, stellar obliquity, and the resulting orbital evolution. In this simplified approach, the young hot Jupiter of one Jupiter mass is modeled as a diamagnetic sphere with a finite resistivity, orbiting across tilted stellar magnetic dipole fields in vacuum. Since the induced ohmic dissipation occurs mostly near the planet's surface, we find that the dissipation is unable to significantly expand the young hot Jupiter. Nevertheless, the planet inside a small corotation orbital radius can undergo orbital decay by the dissipation torque and finally overfill its Roche lobe during the T Tauri star phase. The stellar obliquity can evolve significantly if the magnetic dipole is parallel/antiparallel to the stellar spin. Our results are validated by the general torque-dissipation relation in the presence of the stellar obliquity. We also run the fiducial model of Laine et al. and find that the planet's radius is sustained at a nearly constant value by the ohmic heating, rather than being thermally expanded to the Roche radius as suggested by the authors.

  2. Impact of a Binary System Common Envelope on Mass Transfer through the Inner Lagrange Point

    CERN Document Server

    Bisikalo, D V; Kuznetsov, O A; Chechetkin, V M


    Results of numerical simulations of the impact of a common envelope on the matter flow pattern near the outflowing component in a semidetached binary system are presented. Three-dimensional modeling of the matter transfer gas dynamics in a low-mass X-ray binary X1822-371 enable investigation of the structure of flows in the vicinity of the inner Lagrange point L1. Taking into account the common envelope of the system substantially changes the flow pattern near the Roche surface of the outflowing component. In a stationary regime, accretion of common envelope gas is observed over a significant fraction of the donor star's surface, which inhibits the flow of gas along the Roche surface to L1. The change in the flow pattern is particularly significant near L1, where the stream of common envelope gas strips matter off the stellar surface. This, in turn, significantly increases (by an order of magnitude) the gas flow from the donor surface in comparison with the estimates of standard models.


    Energy Technology Data Exchange (ETDEWEB)

    Chen, Xian; Cuadra, Jorge [Instituto de Astrofísica, Facultad de Física, Pontificia Universidad Católica de Chile, 782-0436 Santiago (Chile); Amaro-Seoane, Pau, E-mail:, E-mail:, E-mail: [Max Planck Institut für Gravitationsphysik (Albert-Einstein-Institut), D-14476 Potsdam (Germany)


    Cold gas entering the central 1–10{sup 2} pc of a galaxy fragments and condenses into clouds. The stability of the clouds determines whether they will be turned into stars or can be delivered to the central supermassive black hole (SMBH) to turn on an active galactic nucleus (AGN). The conventional criteria to assess the stability of these clouds, such as the Jeans criterion and Roche (or tidal) limit, are insufficient here, because they assume the dominance of self-gravity in binding a cloud, and neglect external agents, such as pressure and tidal forces, which are common in galactic nuclei. We formulate a new scheme for judging this stability. We first revisit the conventional Virial theorem, taking into account an external pressure, to identify the correct range of masses that lead to stable clouds. We then extend the theorem to further include an external tidal field, which is equally crucial for the stability in the region of our interest—in dense star clusters, around SMBHs. We apply our extended Virial theorem to find new solutions to controversial problems, namely, the stability of the gas clumps in AGN tori, the circum-nuclear disk in the Galactic Center, and the central molecular zone of the Milky Way. The masses we derive for these structures are orders of magnitude smaller than the commonly used Virial masses (equivalent to the Jeans mass). Moreover, we prove that these clumps are stable, contrary to what one would naively deduce from the Roche (tidal) limit.

  4. A comprehensive photometric study of the eclipsing binary EP Aurigae (United States)

    Li, H.-L.; Wei, J.-Y.; Yang, Y.-G.; Li, K.; Zhang, X.-B.


    We present new observations for the eclipsing binary EP Aurigae, which were performed by using three small telescopes in China from 2003 December to 2014 January. With the updated 2003 version of the Wilson-Devinney code, the photometric elements were deduced from three sets of light curves. Based on all available eclipsing times, the orbital period changes were investigated. It is discovered that the (O-C) curve may show an existence of light-time effect due to an unseen third body, which was weakly identified by the photometric solution. The modulated period and amplitude of the cyclic variation are P3=71.2(±8.0) yr and A=0.0101(±0.0008) day, respectively. In the co-planar orbit with the binary system, the mass of the third body is M3=0.18(±0.02) M⊙. The photometric results imply that EP Aur is an Algol-type binary with a mass ratio of q=0.831(±0.004). Its primary component almost fills its Roche lobe. Therefore, EP Aur may consist of a normal main-sequence star and a cool Roche-lobe filling subgiant, which may be undergoing rapid mass transfer.

  5. Investigation of UBVRI Light Curves of LP Cephei (United States)

    Carrigan, B.; Samec, R. G.; McDermith, R. J.


    In our study of eccentric eclipsing binary candidates (EEB's), we have obtained UBVRI CCD light curves of the LP Cephei, which was discovered by Wachmann (1972). The observations were taken 21 to 25 September 1994, inclusive, at Kitt Peak National Observatory, Arizona. The CCD photometer system (CCDPHOT) was used in conjunction with the 0.9 m Cassegrain reflector telescope. Five mean precision epochs of minimum light were determined from the observations made during primary and secondary eclipses. A period study has been conducted for all available data Analysis of both Wachmann's timings and our timings yielded the following linear ephemeris: HJD Min I = 2 449 621.732 19(78)d + 0.693 0625(1)XE. Despite the reported displacement of the secondary by Wachmann (1972) , we find that the secondary eclipse occurs at 0.5 phase. We have obtained standard magnitudes and estimated the amount of interstellar extinction for LP Cephei. Reddening Estimates indicate that LP Cephei consists of two B-type Near-Contact components. Simultaneous synthetic light curve solutions are discussed. The system is semidetatched: the secondary component fills its Roche lobe, while the primary component has a fillout of ~ 70 % of its Roche lobe.

  6. The coupled effect of tides and stellar winds on the evolution of compact binaries

    CERN Document Server

    Repetto, Serena


    We follow the evolution of compact binaries under the coupled effect of tides and stellar winds until the onset of Roche-lobe overflow. These binaries contain a compact object (either a black-hole, a neutron-star, or a planet) and a stellar component. We integrate the full set of tidal equations, which are based on Hut's model for tidal evolution, and we couple them with the angular momentum loss in a stellar wind. Our aim is twofold. Firstly, we wish to highlight some interesting evolutionary outcomes of the coupling. When tides are coupled with a non-massive stellar wind, one interesting outcome is that in certain types of binaries, the stellar spin tends to reach a quasi-equilibrium state, where the effect of tides and wind are counteracting each other. When tides are coupled with a massive wind, we parametrize the evolution in terms of the decoupling radius, at which the wind decouples from the star. Even for small decoupling radii this \\emph{wind braking} can drive systems on the main sequence to Roche-l...

  7. Performance comparison of next-generation sequencing platforms for determining HIV-1 coreceptor use (United States)

    Raymond, Stéphanie; Nicot, Florence; Jeanne, Nicolas; Delfour, Olivier; Carcenac, Romain; Lefebvre, Caroline; Cazabat, Michelle; Sauné, Karine; Delobel, Pierre; Izopet, Jacques


    The coreceptor used by HIV-1 must be determined before a CCR5 antagonist, part of the arsenal of antiretroviral drugs, is prescribed because viruses that enter cells using the CXCR4 coreceptor are responsible for treatment failure. HIV-1 tropism is also correlated with disease progression and so must be determined for virological studies. Tropism can be determined by next-generation sequencing (NGS), but not all of these new technologies have been fully validated for use in clinical practice. The Illumina NGS technology is used in many laboratories but its ability to predict HIV-1 tropism has not been evaluated while the 454 GS-Junior (Roche) is used for routine diagnosis. The genotypic prediction of HIV-1 tropism is based on sequencing the V3 region and interpreting the results with an appropriate algorithm. We compared the performances of the MiSeq (Illumina) and 454 GS-Junior (Roche) systems with a reference phenotypic assay. We used clinical samples for the NGS tropism predictions and assessed their ability to quantify CXCR4-using variants. The data show that the Illumina platform can be used to detect minor CXCR4-using variants in clinical practice but technical optimization are needed to improve quantification. PMID:28186189

  8. Obinutuzumab: first global approval. (United States)

    Cameron, Fiona; McCormack, Paul L


    Obinutuzumab (Gazyva™) is an intravenously administered, humanized and glycoengineered, type II anti-CD20 monoclonal antibody for the treatment of B-cell malignancies. It is approved in the US for use in combination with chlorambucil for the first-line treatment of chronic lymphocytic leukaemia (CLL), and has been filed for approval in the EU in this indication. The antibody is based on GlycArt Biotechnology's (later Roche Glycart AG) proprietary GlycoMAb® technology, which uses glycoengineered antibodies that specifically increase antibody-dependent cellular cytotoxicity and thereby increase immune-mediated target cell death. Obinutuzumab is a type II anti-CD20 antibody that induces enhanced direct cell death. The monoclonal antibody is in worldwide phase III development with Roche and its subsidiaries, Genentech and Chugai Pharmaceutical, as well as Biogen Idec, for diffuse large B-cell lymphoma and non-Hodgkin's lymphoma generally, and is also in phase III development in countries outside of the US and EU for CLL.

  9. [Historical study of delirium tremens in Spain in the second half of the 19th century]. (United States)

    Conde Lopez, V J; Plaza Nieto, J F; Macias Fernandez, J A


    The authors, after a brief historical introduction, review the clinical contributions about Delirium Tremens during the second half of the XIXth century in 9 Saniard authors: J. Santamaría del Alba (1952), Inocente Escudero (1857), R. Sanfrutos (1858), Tomás Santero y Moreno (1867), Candela (1871), Robert (1871), José Armesto (1877), J. M. Castañeda (1879-1880), R. B. de la Roche (1881). It is appropriate to underline their tidying up and clinical interest, medical-pathological and general, their interest in the evolutionary course, their therapeutical empiricism and a certain indifference in framing this nosological entity in a doctrinal corpus more specific, except for Robert, who use the chloral hydrate only two after its introduction in medicine by Liebreich and De la Roche, who complains about absence of a monographic study and explains it according to the anatomic-clinical model of the General Pathology of his epoch, in a appropriate bibliographical context. Included are 12 tables and 27 bibliographical references.

  10. Stability of Gas Clouds in Galactic Nuclei: An Extended Virial Theorem (United States)

    Chen, Xian; Amaro-Seoane, Pau; Cuadra, Jorge


    Cold gas entering the central 1-102 pc of a galaxy fragments and condenses into clouds. The stability of the clouds determines whether they will be turned into stars or can be delivered to the central supermassive black hole (SMBH) to turn on an active galactic nucleus (AGN). The conventional criteria to assess the stability of these clouds, such as the Jeans criterion and Roche (or tidal) limit, are insufficient here, because they assume the dominance of self-gravity in binding a cloud, and neglect external agents, such as pressure and tidal forces, which are common in galactic nuclei. We formulate a new scheme for judging this stability. We first revisit the conventional Virial theorem, taking into account an external pressure, to identify the correct range of masses that lead to stable clouds. We then extend the theorem to further include an external tidal field, which is equally crucial for the stability in the region of our interest—in dense star clusters, around SMBHs. We apply our extended Virial theorem to find new solutions to controversial problems, namely, the stability of the gas clumps in AGN tori, the circum-nuclear disk in the Galactic Center, and the central molecular zone of the Milky Way. The masses we derive for these structures are orders of magnitude smaller than the commonly used Virial masses (equivalent to the Jeans mass). Moreover, we prove that these clumps are stable, contrary to what one would naively deduce from the Roche (tidal) limit.


    Energy Technology Data Exchange (ETDEWEB)

    Fossati, L.; Haswell, C. A.; Patel, M. R.; Busuttil, R. [Department of Physical Sciences, Open University, Walton Hall, Milton Keynes MK7 6AA (United Kingdom); Bagnulo, S. [Armagh Observatory, College Hill, Armagh BT61 9DG (United Kingdom); Kowalski, P. M. [GFZ German Research Centre for Geosciences, Telegrafenberg, D-14473 Potsdam (Germany); Shulyak, D. V. [Institute of Astrophysics, Georg-August-University, Friedrich-Hund-Platz 1, D-37077 Goettingen (Germany); Sterzik, M. F., E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail:, E-mail: [European Southern Observatory, Casilla 19001, Santiago 19 (Chile)


    Since there are several ways planets can survive the giant phase of the host star, we examine the habitability and detection of planets orbiting white dwarfs. As a white dwarf cools from 6000 K to 4000 K, a planet orbiting at 0.01 AU would remain in the continuous habitable zone (CHZ) for {approx}8 Gyr. We show that photosynthetic processes can be sustained on such planets. The DNA-weighted UV radiation dose for an Earth-like planet in the CHZ is less than the maxima encountered on Earth, and hence non-magnetic white dwarfs are compatible with the persistence of complex life. Polarization due to a terrestrial planet in the CHZ of a cool white dwarf (CWD) is 10{sup 2} (10{sup 4}) times larger than it would be in the habitable zone of a typical M-dwarf (Sun-like star). Polarimetry is thus a viable way to detect close-in rocky planets around white dwarfs. Multi-band polarimetry would also allow us to reveal the presence of a planet atmosphere, providing a first characterization. Planets in the CHZ of a 0.6 M{sub Sun} white dwarf will be distorted by Roche geometry, and a Kepler-11d analog would overfill its Roche lobe. With current facilities a super-Earth-sized atmosphereless planet is detectable with polarimetry around the brightest known CWD. Planned future facilities render smaller planets detectable, in particular by increasing the instrumental sensitivity in the blue.


    Energy Technology Data Exchange (ETDEWEB)

    Kremer, Kyle; Kalogera, Vassiliki [Center for Interdisciplinary Exploration and Research in Astrophysics (CIERA), Department of Physics and Astronomy, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208 (United States); Sepinsky, Jeremy, E-mail:, E-mail:, E-mail: [Department of Physics and Electrical Engineering, The University of Scranton Scranton, PA 18510 (United States)


    We calculate the long-term evolution of angular momentum in double white dwarf binaries undergoing direct impact accretion over a broad range of parameter space. We allow the rotation rate of both components to vary and account for the exchange of angular momentum between the spins of the white dwarfs and the orbit, while conserving the total angular momentum. We include gravitational, tidal, and mass transfer effects in the orbital evolution, and allow the Roche radius of the donor star to vary with both the stellar mass and the rotation rate. We examine the long-term stability of these systems, focusing in particular on those systems that may be progenitors of AM CVn or SNe Ia. We find that our analysis yields an increase in the predicted number of stable systems compared to that in previous studies. Additionally, we find that by properly accounting for the effects of asynchronism between the donor and the orbit on the Roche-lobe size, we eliminate oscillations in the orbital parameters, which were found in previous studies. Removing these oscillations can reduce the peak mass transfer rate in some systems, keeping them from entering an unstable mass transfer phase.

  13. Long-term Evolution of Double White Dwarf Binaries Accreting through Direct Impact (United States)

    Kremer, Kyle; Sepinsky, Jeremy; Kalogera, Vassiliki


    We calculate the long-term evolution of angular momentum in double white dwarf binaries undergoing direct impact accretion over a broad range of parameter space. We allow the rotation rate of both components to vary and account for the exchange of angular momentum between the spins of the white dwarfs and the orbit, while conserving the total angular momentum. We include gravitational, tidal, and mass transfer effects in the orbital evolution, and allow the Roche radius of the donor star to vary with both the stellar mass and the rotation rate. We examine the long-term stability of these systems, focusing in particular on those systems that may be progenitors of AM CVn or SNe Ia. We find that our analysis yields an increase in the predicted number of stable systems compared to that in previous studies. Additionally, we find that by properly accounting for the effects of asynchronism between the donor and the orbit on the Roche-lobe size, we eliminate oscillations in the orbital parameters, which were found in previous studies. Removing these oscillations can reduce the peak mass transfer rate in some systems, keeping them from entering an unstable mass transfer phase.

  14. An efficient simulator of 454 data using configurable statistical models

    Directory of Open Access Journals (Sweden)

    Persson Bengt


    Full Text Available Abstract Background Roche 454 is one of the major 2nd generation sequencing platforms. The particular characteristics of 454 sequence data pose new challenges for bioinformatic analyses, e.g. assembly and alignment search algorithms. Simulation of these data is therefore useful, in order to further assess how bioinformatic applications and algorithms handle 454 data. Findings We developed a new application named 454sim for simulation of 454 data at high speed and accuracy. The program is multi-thread capable and is available as C++ source code or pre-compiled binaries. Sequence reads are simulated by 454sim using a set of statistical models for each chemistry. 454sim simulates recorded peak intensities, peak quality deterioration and it calculates quality values. All three generations of the Roche 454 chemistry ('GS20', 'GS FLX' and 'Titanium' are supported and defined in external text files for easy access and tweaking. Conclusions We present a new platform independent application named 454sim. 454sim is generally 200 times faster compared to previous programs and it allows for simple adjustments of the statistical models. These improvements make it possible to carry out more complex and rigorous algorithm evaluations in a reasonable time scale.

  15. Elements of Poro-Elasticity for Reservoir Engineering Éléments de poro-élasticité pour l'ingénierie de réservoirs

    Directory of Open Access Journals (Sweden)

    Bouteca M.


    -élasticité utiles à l'ingénieur de réservoir. La poro-élasticité permet de calculer les déformations du solide liées au mouvement du fluide. C'est donc le cadre théorique qui permet de définir la compressibilité de la roche. Nous montrons ainsi, dans un premier temps, comment les variables mécaniques précédemment introduites sont reliées à la compressibilité de la roche mesurée expérimentalement. Deux hypothèses sont retenues : a mesure en condition isotrope (définie par une pression de confinement et une pression de pore, b mesure en condition oedométrique (définie par un chargement vertical, un déplacement radial nul et une pression de pore. Les principaux résultats sont illustrés dans le tableau 6. On montre en outre qu'en condition isotrope, la compressibilité du volume poreux peut être mesurée indifféremment à pression de pore variable et pression de confinement constante ou à pression de pore constante et pression de confinement variable (tableau 5. Dans un deuxième temps nous examinons sous quelles conditions l'équation de diffusivité définie dans la première partie peut être découplée de l'équation d'équilibre du solide. On montre que la contribution de la déformation de la roche à l'écoulement peut être exprimée à l'aide d'un coefficient de compressibilité à condition que l'on fasse des hypothèses sur le mode de déformation local de la roche. Rappelons que, par opposition à des conditions aux limites qui sont vraies sur le contour du domaine, les conditions locales sont vraies en tout point et constituent une simplification du problème réel. En conclusion, on souligne l'importance du choix des conditions locales retenues pour la mesure de la compressibilité de la roche. La compressibilité de la roche étant définie, l'équation de diffusivité prend sa forme classique : la contribution de la roche est introduite en définissant une compressibilité totale somme de la compressibilité du fluide et de celle de la roche.

  16. Dissection of the octoploid strawberry genome by deep sequencing of the genomes of Fragaria species. (United States)

    Hirakawa, Hideki; Shirasawa, Kenta; Kosugi, Shunichi; Tashiro, Kosuke; Nakayama, Shinobu; Yamada, Manabu; Kohara, Mistuyo; Watanabe, Akiko; Kishida, Yoshie; Fujishiro, Tsunakazu; Tsuruoka, Hisano; Minami, Chiharu; Sasamoto, Shigemi; Kato, Midori; Nanri, Keiko; Komaki, Akiko; Yanagi, Tomohiro; Guoxin, Qin; Maeda, Fumi; Ishikawa, Masami; Kuhara, Satoru; Sato, Shusei; Tabata, Satoshi; Isobe, Sachiko N


    Cultivated strawberry (Fragaria x ananassa) is octoploid and shows allogamous behaviour. The present study aims at dissecting this octoploid genome through comparison with its wild relatives, F. iinumae, F. nipponica, F. nubicola, and F. orientalis by de novo whole-genome sequencing on an Illumina and Roche 454 platforms. The total length of the assembled Illumina genome sequences obtained was 698 Mb for F. x ananassa, and ∼200 Mb each for the four wild species. Subsequently, a virtual reference genome termed FANhybrid_r1.2 was constructed by integrating the sequences of the four homoeologous subgenomes of F. x ananassa, from which heterozygous regions in the Roche 454 and Illumina genome sequences were eliminated. The total length of FANhybrid_r1.2 thus created was 173.2 Mb with the N50 length of 5137 bp. The Illumina-assembled genome sequences of F. x ananassa and the four wild species were then mapped onto the reference genome, along with the previously published F. vesca genome sequence to establish the subgenomic structure of F. x ananassa. The strategy adopted in this study has turned out to be successful in dissecting the genome of octoploid F. x ananassa and appears promising when applied to the analysis of other polyploid plant species.

  17. IROme, a new high-throughput molecular tool for the diagnosis of inherited retinal dystrophies. (United States)

    Schorderet, Daniel F; Iouranova, Alexandra; Favez, Tatiana; Tiab, Leila; Escher, Pascal


    The molecular diagnosis of retinal dystrophies is difficult because of the very important number of genes implicated and is rarely helped by genotype-phenotype correlations. This prompted us to develop IROme, a custom designed in solution-based targeted exon capture assay (SeqCap EZ Choice library, Roche NimbleGen) for 60 retinitis pigmentosa-linked genes and three candidate genes (942 exons). Pyrosequencing was performed on a Roche 454 GS Junior benchtop high-throughput sequencing platform. In total, 23 patients affected by retinitis pigmentosa were analyzed. Per patient, 39.6 Mb were generated, and 1111 sequence variants were detected on average, at a median coverage of 17-fold. After data filtering and sequence variant prioritization, disease-causing mutations were identified in ABCA4, CNGB1, GUCY2D, PROM1, PRPF8, PRPF31, PRPH2, RHO, RP2, and TULP1 for twelve patients (55%), ten mutations having never been reported previously. Potential mutations were identified in 5 additional patients, and in only 6 patients no molecular diagnosis could be established (26%). In conclusion, targeted exon capture and next-generation sequencing are a valuable and efficient approach to identify disease-causing sequence variants in retinal dystrophies.

  18. A puzzling millisecond pulsar companion in NGC 6266

    CERN Document Server

    Cocozza, G; Possenti, A; Beccari, G; Lanzoni, B; Ranson, S; Rood, R T; D'Amico, N


    We report on the optical identification of the companion to the eclipsing millisecond pulsar PSR J1701$-$3006B in the globular cluster NGC 6266. A relatively bright star with an anomalous red colour and an optical variability ($\\sim$ 0.2 mag) that nicely correlates with the orbital period of the pulsar ($\\sim$ 0.144 days) has been found nearly coincident with the pulsar nominal position. This star is also found to lie within the error box position of an X-ray source detected by Chandra observations, thus supporting the hypothesis that some interaction is occurring between the pulsar wind and the gas streaming off the companion. Although the shape of the optical light curve is suggestive of a tidally deformed star which has nearly completely filled its Roche lobe, the luminosity ($\\sim 1.9 L_\\odot$) and the surface temperature ($\\sim 6000$ K) of the star, deduced from the observed magnitude and colours, would imply a stellar radius significantly larger than the Roche lobe radius. Possible explanations for this...

  19. Spin Evolution of Neutron Stars in OB/X-ray Binaries

    Institute of Scientific and Technical Information of China (English)

    Fan Zhang; Xiang-Dong Li; Zhen-Ru Wang


    We have investigated the relation between the orbital period Porb and the spin period Ps of neutron stars in OB/X-ray binaries.By simulating the timedevelopment of the mass loss rate and radius expansion of a 20M(◎)donor star,we have calculated the detailed spin evolution of the neutron star before steady wind accretion occurs(that is,when the break spin period is reached),or when the OB star begins evolving off the main sequence or has filled its Roche lobe.Our results are compatible with the observations of OB/X-ray binaries.We find that in relatively narrow systems with orbital periods less than tens of days,neutron stars with initial magnetic field B0 stronger than about 3 × 1012 G can reach the break spin period to allow steady wind accretion in the main sequence time,whereas neutron stars with B0 < 3 × 1012 G and/or in wide systems would still be in one of the pulsar,rapid rotator or propeller phases when the companion evolves off the main sequence or fills its Roche lobe.Our results may help understand the various characteristics of the observed OB/neutron star binaries along with their distributions in the Ps -Porb diagram.

  20. Read length versus depth of coverage for viral quasispecies reconstruction.

    Directory of Open Access Journals (Sweden)

    Osvaldo Zagordi

    Full Text Available Recent advancements of sequencing technology have opened up unprecedented opportunities in many application areas. Virus samples can now be sequenced efficiently with very deep coverage to infer the genetic diversity of the underlying virus populations. Several sequencing platforms with different underlying technologies and performance characteristics are available for viral diversity studies. Here, we investigate how the differences between two common platforms provided by 454/Roche and Illumina affect viral diversity estimation and the reconstruction of viral haplotypes. Using a mixture of ten HIV clones sequenced with both platforms and additional simulation experiments, we assessed the trade-off between sequencing coverage, read length, and error rate. For fixed costs, short Illumina reads can be generated at higher coverage and allow for detecting variants at lower frequencies. They can also be sufficient to assess the diversity of the sample if sequences are dissimilar enough, but, in general, assembly of full-length haplotypes is feasible only with the longer 454/Roche reads. The quantitative comparison highlights the advantages and disadvantages of both platforms and provides guidance for the design of viral diversity studies.