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Sample records for amphioxus branchiostoma floridae

  1. Taxonomy Icon Data: Florida lancelet (amphioxus) [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Florida lancelet (amphioxus) Branchiostoma floridae Chordata/Urochordata,Cephalochorda...ta Branchiostoma_floridae_L.png Branchiostoma_floridae_NL.png Branchiostoma_floridae_S.png Branchiostoma_florida...e_NS.png http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Branchiostoma+floridae&t=L http://bioscienc...edbc.jp/taxonomy_icon/icon.cgi?i=Branchiostoma+floridae&t=NL http://biosciencedbc....jp/taxonomy_icon/icon.cgi?i=Branchiostoma+floridae&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Branchiostoma+florida

  2. Evidence for stasis and not genetic piracy in developmental expression patterns of Branchiostoma lanceolatum and Branchiostoma floridae, two amphioxus species that have evolved independently over the course of 200 Myr.

    Science.gov (United States)

    Somorjai, Ildiko; Bertrand, Stéphanie; Camasses, Alain; Haguenauer, Anne; Escriva, Hector

    2008-12-01

    Cephalochordates, the most basal extant group in the phylum Chordata, are represented chiefly by about 20 species of the genus Branchiostoma, commonly called amphioxus or lancelets. In recent years, insights into the evolutionary origin of the vertebrates have been gained from molecular genetic studies during the development of three of these amphioxus species (Branchiostoma floridae in North America, Branchiostoma lanceolatum in Europe, and Branchiostoma belcheri in East Asia). In spite of an estimated divergence time of 100-200 Myr among these species, all three are remarkably similar morphologically, and students of amphioxus have tacitly assumed that such resemblances arise during ontogeny from nearly identical networks of developmental genes. We felt that this assumption needed to be reexamined because instances are known--even in comparisons of closely related species--where characters seeming homologous on the basis of morphology actually develop under the control of conspicuously divergent genetic programs (a phenomenon termed "genetic piracy"). In the present work, we tested the hypothesis that morphological similarities reflect strict conservation of developmentally important genes' expression patterns in order to assess whether the developmental genetics of different amphioxus species show evidence of genetic piracy. To these ends, we cloned 18 genes implicated in different developmental functions in B. lanceolatum and compared their gene expression patterns with the known expression patterns of their orthologous genes in B. floridae. We show that, for the most part, conservation of gene expression parallels that of morphology in these two species. We also identified some differences in gene expression, likely reflecting experimental sensitivity, with the exception of Pax1/9, which may result from true developmental specificities in each amphioxus species. Our results demonstrate that morphological conservation reflects stasis in developmental gene

  3. Cryopreservation of amphioxus ( Branchiostoma belcheri) spermatozoa

    Institute of Scientific and Technical Information of China (English)

    XU Yuyan; ZHANG Shicui; VERAPONG Vuthiphandchai; SUBUNTITH Nimrat

    2009-01-01

    Amphioxus, Branchiostoma belcheri, a closest relative of vertebrates, is at a high risk of extinction due to a combination of low effective population size, altered native habitats and environmental pollution, yet little is known about cryopreservation of its gametes. This study deals with the cryopreservation of amphioxus semen. The main findings are that (1) the extender of Yao et al. is the best one among the four extenders examined; (2) the appropriate ratio of semen to extender of Yao et al. plus cryoprotectant is from 1:5 to 1:7; (3) dimethyl sulfoxide (DMSO) and methanol are the better cryoprotectants than glycerol, with DMSO giving the best results; (4) the eggs fertilized with post-thaw spermatozoa are capable of developing to at least hatching stage, and the highest hatching rate is (12.4±3.0)%. This is the first report on freezing and thawing of amphioxus spermatozoa,providing a simple and practical protocol for cryopreservation of amphioxus spermatozoa and laying a foundation for safeguarding this endangered species.

  4. ARTIFICIAL CULTURE OF AMPHIOXUS (BRANCHIOSTOMA BELCHERI TSINGTAUENSE)

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    Mature amphioxus (Branchiostoma belcheri tsingtauense) collected by trawl in June, 1994 from Qingdao Shazikou sea area bottom sands, were cultured under controlled indoor conditions, where they spawned and their eggs were fertilized and hatched into larvae which gradually settled at the bottom and dug into the sands after 40-50 days culture in a water trough outdoors. The total survival rate of the larvae(LSR)was 5.5% before they went into the sands, 1.9% in 4 months, 0.7% in 10 months. The survival rate of the young fish which had dug into the sands (YSR) was 35.6% in 4 months, and 12.6% in 10 months. The amphioxus number tended to be constant from the 11th month on. In the first five months after incubation, the amphioxus body length increased by an average of about 1.5 mm a month, and about 0.4 mm a month from Dec. to May of next year. The maximum length after ten months was 24 mm; the average was about 11 mm. After two years culture in the water trough, the maximum length could reach 34-35 mm, when the amphioxus gonads began to develop.

  5. Synergistic actions of complement and lysozyme in clearance of Escherichia coli from amphioxus Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    Guangfeng Wang; Shicui Zhang; Zhimeng Zhuang; Zhiping Wang

    2009-01-01

    Amphioxus appears to lack free circulating blood cells. How it clears invading pathogens from its body remains unknown to date. We demonstrate here that amphioxus Branchiostoma belcheri is capable of efficiently eliminating the invading bacterium Escherichia coil from its humorai fluid, and the complement and lysozyme are both involved in the elimination of the invading pathogen. Both the com-plement and lysozyme act in concert against the invading bacterium, but the complement appears to play a more dominant role than the lysozyme.

  6. Antioxidant enzyme activities in different genders and tissues of amphioxus Branchiostoma belcheri tsingtauense

    Institute of Scientific and Technical Information of China (English)

    WEI Ran; ZHANG Shicui; WANG Changfa; PANG Qiuxiang

    2007-01-01

    Information regarding antioxidant enzymes in amphioxus remains lacking, and this study was carried out to examine the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) in different genders and tissues of amphioxus Branchiostoma belcheri tsingtauense.Results show that (1) CuZn-SOD, CAT and GPX activities in the whole amphioxus B. belcheri tsingtauense were basically at the same levels in male and female amphioxus, whereas both T-SOD and Mn-SOD activities in male amphioxus were significantly higher than that in the female (P<0.05); (2) The testis had significantly higher T-SOD and CuZn-SOD activities than the ovary (P<0.05); (3) CuZn-SOD activity was undetectable in the guts of male and female amphioxus; (4) For both male and female amphioxus, the activities of CAT and GPX in the gonads including testis and ovary were the lowest (P<0.05)among the tissues examined; (5) The gut and gill had the same level GPX activities while the gut had a higher CAT activity; (6) There was no clear difference in CAT and GPX activities in the corresponding tissues between male and female amphioxus. The study on SOD, CAT and GPX activities in different genders and tissues of the protochordate provides data for future comparison of amphioxus antioxidant enzymes with those of invertebrates and vertebrates.

  7. N-Methyl-D-aspartic Acid (NMDA in the nervous system of the amphioxus Branchiostoma lanceolatum

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    Garcia-Fernàndez Jordi

    2007-12-01

    Full Text Available Abstract Background NMDA (N-methyl-D-aspartic acid is a widely known agonist for a class of glutamate receptors, the NMDA type. Synthetic NMDA elicits very strong activity for the induction of hypothalamic factors and hypophyseal hormones in mammals. Moreover, endogenous NMDA has been found in rat, where it has a role in the induction of GnRH (Gonadotropin Releasing Hormone in the hypothalamus, and of LH (Luteinizing Hormone and PRL (Prolactin in the pituitary gland. Results In this study we show evidence for the occurrence of endogenous NMDA in the amphioxus Branchiostoma lanceolatum. A relatively high concentration of NMDA occurs in the nervous system of this species (3.08 ± 0.37 nmol/g tissue in the nerve cord and 10.52 ± 1.41 nmol/g tissue in the cephalic vesicle. As in rat, in amphioxus NMDA is also biosynthesized from D-aspartic acid (D-Asp by a NMDA synthase (also called D-aspartate methyl transferase. Conclusion Given the simplicity of the amphioxus nervous and endocrine systems compared to mammalian, the discovery of NMDA in this protochordate is important to gain insights into the role of endogenous NMDA in the nervous and endocrine systems of metazoans and particularly in the chordate lineage.

  8. Localization of nitric oxide synthase in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The localization of nitric oxide synthases (NOS) is dealed with in the developing gonads of amphioxus Branchiostoma belcheri tsingtauense. It was found by NADPH-diaphorase staining that (1) NOS activity was present in the nuclear membranes of germinal vesicles during the entire period of oocyte development; (2) NOS was localized in both the nuclear membranes and the perinuclear region of cytoplasm in the vitellogenetic oocytes; (3) NOS was relocated in the cortical layer in the mature egg; (4) NOS activity was present in spermatocytes, but not in the spermatogonia in the middle of October; (5) NOS was detected in both spermatozoa and spermatids as well as spermatocytes during the breeding season. This is the first report on the distribution pattern of NOS in the developing gonads in protochordates. These results suggest a role for NOS in the functioning of the nuclear membranes and yolk synthesis during oogenesis and in cell division and differentiation during spermatogenesis.

  9. Distribution of ghrelin-ike immunoreactive cells in amphioxus, Branchiostoma belcheri- A study of immunohistochemistry

    Institute of Scientific and Technical Information of China (English)

    You-Zhu Weng; Hai-Xia Song; Yong-Qiang Fang

    2008-01-01

    The distribution of ghrelin-like immunoreactive cells in amphioxus (Branchiostoma belcheri) was investigated by using immunohisto-chemical staining with rabbit antiserum against synthetical mammalian ghrelin. The results showed that ghrelin-like immunoreactive cells were distributed widely in the nervous system, Hatschek's pit, wheel organ, digestive tract and gonads (ovary and testis). In nervous system, ghrelin-like immunoreactive neurons and their protrusions were distributed specifically on the dorsal side, ventral side and funnel part of brain vesicle, with a few dispersive immunoreactive nerve cells and their fibers in nerve tube. Ghrelin-like immunoreactivities were also detected in Hatschek's pit epithelial cells and wheel organ cells, with positive substance located along cell membrane. In digestive tract, ghrelin-like immunoreactive cells existed in hepatic diverticulum, anterior and posterior region of midgut, and could be classified into two types, closed- and opened-type endocrine cells. The number of positive cells was most in hepatic diverticulum, secondary in posterior region of midgut and least in anterior region of midgut. In gonads, ghrelin-like immunoreactive substance was detected in oogonia, oocytes and follicle cells in ovary at the small and large growth stages and in early spermatogenic cells and Sertoli cells in testis. The extensive distribution of ghrelin-like cells in amphioxus suggested that these kinds of cells are conservative in evolution and diversified in function. At the same time, we found for the first time that ghrelin-like immunoreactive cells existed in brain vesicle and Hatschek's pit, which provided new morphological evidence for the existence of an activation pathway between brain vesicle and Hatschek's pit for the regulation of growth hormone excretion.

  10. Actin gene family in Branchiostoma belched

    Institute of Scientific and Technical Information of China (English)

    2016-01-01

    Actin is a highly conserved cytoskeletal protein that is found in essentially all eukaryotic cells,which plays a paramount role in several basic functions of the organism, such as the maintenance of cellshape, cell division, cell mobility and muscle contraction. However, little is known about actin gene family inChinese amphioxusBranchiostoma belcheri). Here we systemically analyzed the actin genes family inBranchiostoma belched and found that amphioxus contains 33 actin genes. These genes have undergoneextensive expansion through tandem duplications by phylogenetic analysis. In addition, we also providedevidence indicating that actin genes have divergent functions by specializing their EST data in both Bran-chiostoma belched and Branchiostoma florida. Our results provided an alternative explanation for the evolu-tion of actin genes, and gave new insights into their functional roles.

  11. Characterization of SoxB2 and SoxC genes in Amphioxus (Branchiostoma belcheri): Implications for their evolutionary conservation

    Institute of Scientific and Technical Information of China (English)

    LIN YuShuang; CHEN DongYan; FAN QiuSheng; ZHANG HongWei

    2009-01-01

    Most Sox genes directly affect cell fate determination and differentiation. In this study, we isolated two Sox genes: SoxB2 and SoxC from amphioxus (Branchiostoma belcheri), the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches, suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation, they are expressed in the neural ectoderm and archenterons. In adults, they are expressed not only in the nerve cord, but also in the gut, midgut diverticulum, gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous sys-tem and gonads as their vertebrate homologs.

  12. A sperm-mediated GFP gene transfer in amphioxus (Branchiostoma belcheri tsingtaoense)

    Institute of Scientific and Technical Information of China (English)

    YU Dan; ZHANG Peijun

    2005-01-01

    @@ The cephalochordate amphioxus is the closest living invertebrate relative of the vertebrate and has emerged as an important animal model for evolutionary developmental biology research[1]. Many insights into the evolution of the vertebrate body plan can be gained by comparing vertebrate embryogenesis with that of the amphioxus. Investigation of the gene regulation during amphioxus embryogenesis will provide us clues to clarify how changes of gene regulation affect the body plan evolution at this turning point and thus will throw light on the origin and evolution of the vertebrate.

  13. Study on genetic diversity and resource conservation of amphioxus(Branchiostoma balcheri Gray) population

    Institute of Scientific and Technical Information of China (English)

    ZHOU Hantao; GAO Yuanyuan; CHEN Xu; YE Fan; LIAN Yuwu

    2005-01-01

    Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates.Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNAs (RAPDs) technologies were applied to detect the genetic variation of 3 bulking samples and individually sampled populations in nowadays Xiamen sea areas (Xiekou, Nanxian and Huangcuo) where the amphioxuses are alive. For the bulking sampled populations, 5 ISSR and 10 RAPD primers generated 357bands, of which 181 (50.7%) were polymorphic. Nei index and UPGMA statistical analysis indicated that amphioxuses in these 3areas could be divided into 2 groups. The genetic distance between animals in Nanxian and Huangcuo areas was 0.07 and classified into 1 group, while the population in Xiekou belonged to another group because its genetic distances in Nanxian and Huangcuo were 0.12 and 0.14, respectively. The result was in accordance with the morphological comparison among animals from those areas. For individually sampled population, Shannon' s index of genetic diversity was used to partition the diversity of the animals among these 3sea areas, and the results showed that the indices in populations ofXiekou, Nanxian and Huangcuo, were 0.583, 0.482 and 0.374,respectively. The linear regression equation analysis for amphioxus' genetic diversity versus the environment factors revealed that granularity/sorting coefficient and water depth were the most important factors that affect amphioxus genetic diversity. On the basis of the results, the suggestions for amphioxus resource conservation in Xiamen sea areas are put forward.

  14. Ribosomal Protein Genes S23 and L35 from Amphioxus Branchiostoma belcheri tsingtauense: Identification and Copy Number

    Institute of Scientific and Technical Information of China (English)

    Xian LI; Shi-Cui ZHANG; Zhen-Hui LIU; Hong-Yan LI

    2005-01-01

    The complete cDNA and deduced amino acid sequences of the ribosomal proteins S23 (AmphiS23) and L35 (AmphiL35) from amphioxus Branchiostoma belcheri tsingtauense were identified in this study. AmphiS23 cDNA is 546 bp long and encodes a protein of 143 amino acids. It has a predicted molecular mass of 15,851 Da and a pI of 10.7. AmphiL35 cDNA comprises 473 bp, and codes for a protein of 123 amino acids with a predicted molecular mass of 14,543 Da and a pI of 10.8. AmphiS23 shares more than 83% identity with its homologues in the vertebrates and more than 84% identity with those in the invertebrates. AmphiL35 is more than 63% identical to its counterparts in the vertebrates and more than 52% identical to those in the invertebrates, Southern blot analysis demonstrated the existence of 1-2 copies of the S23 gene and 2-3 copies of the L35 gene in the genome of amphioxus B. belcheri tsingtauense. This is in sharp contrast to the presence of 6-13 copies of the S23 gene and 15-17 copies of the L35 gene in the rat genome. It is clear that the housekeeping genes like S23 and L35 underwent a large-scale duplication in the vertebrate lineage, reinforcing the gene/genome duplication hypothesis.

  15. Unique Asymmetric Protrusion of Nerve Cord in the Amphioxus, Branchiostoma belcheri

    Science.gov (United States)

    Nozaki, Masumi; Terakado, Kiyoshi; Kubokawa, Kaoru

    The amphioxus is the only surviving prevertebrate segmented chordate. In this animal Hatschek's pit has long been regarded as a putative homologue of the adenohypophysis because of the presence of secretory granules and immunoreactive cells to vertebrate gonadotrophic hormone in this organ. We found that the nerve cord extends a protrusion to the pit along the right side of the notochord. Furthermore, secretory granules were found not only in the pit but also in the protrusion of the nerve cord. These results suggest that Hatschek's pit and the nerve protrusion are homologous to the adenohypophysis and neurohypophysis, respectively. We believe that this is an evidence for the presence of the neuroendocrine link between the central nervous system and Hatschek's pit in the amphioxus.

  16. Neuroendocrine regulation of structure and function in the endostyle of amphioxus, Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Using histological and histochemical methods the structure of the endostyle in amphioxus at different gonadal developmental stages is studied, and the immunocytochemical localization of thyroglobulin (Tg) and thyrotropin (TSH) in the endostyle, Hatschek's pit and brain vesicle is investigated. The results not only confirm the previous report that the endostyle is composed of 6 zones and the cells of zone 5 are thyroid hormone synthesizing cells, but also find the thyroxine synthesis in and secretion from zone 3. In addition, the epithelial cells in Hatschek's pit and the neural cells in brain vesicle are immunopositive for TSH, and the immunoactivity is correlated with gonadal cycle. The present study may provide morphological proof for the hypothesis that the secretory activity of thyroid cells is regulated by TSH from Hatschek's pit and brain vesicle.

  17. Characterization of SoxB2 and SoxC genes in Amphi-oxus (Branchiostoma belcheri):Implications for their evolutionary conservation

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Most Sox genes directly affect cell fate determination and differentiation. In this study,we isolated two Sox genes:SoxB2 and SoxC from amphioxus (Branchiostoma belcheri),the closest living invertebrate relative of the vertebrates. Alignments of SoxB2 and SoxC protein sequences and their vertebrate homologs show high conservation of their HMG domains. Phylogenic analysis shows that amphioxus SoxB2 and SoxC fall out of the vertebrate branches,suggesting that vertebrate homologs might arise from gene duplications during evolution. The two genes possess similar spatial and temporal expression patterns during embryogenesis and in adults. They are both maternally inherited. During neurulation,they are expressed in the neural ectoderm and archenterons. In adults,they are expressed not only in the nerve cord,but also in the gut,midgut diverticulum,gill and oocytes. These results suggest that amphioxus SoxB2 and SoxC might co-function and have conserved functions in the nervous system and gonads as their vertebrate homologs.

  18. Distribution of α-MSH-like immunoreactive cells in the nervous system, Hatschek's pit and other tissues of amphioxus, Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    Yongqiang Fang; Youzhu Weng; Haixia Song

    2008-01-01

    Immunohistochemical localization of a-melanocyte-stimulating hormone (α-MSH) in the nervous system, Hatschek's pit and other tissues of amphioxus (Branchiostoma belcheri) was performed using the antibody against synthetic α-MSH. The results revealed that α-MSH-like immunoreactive cells were distributed at the dorsal side and ventral side of brain vesicle, the dorsal side and the surrounding of nerve tube, and in the epithelial cells of Hatschek's pit, the zone 1, 3, and 6 of endostyle and gut. The immunoreactive substance was also found in the primary oocytes of the small and large growth stage of ovary and early stage spermatogenic cells in testis. These findings indicate that α-MSH is an ancient and highly conserved hormone and it is extensively distributed in amphioxus. Although Hatschek's pit in amphioxus does not have a structure of the intermediate lobe of vertebrate adenohypophysis, it has already hosted α-MSH-like endocrine cells, implying that the functional differentiation of α-MSH-like cells occurred earlier than the differentiation of the tissue structure. The results of the present study provided a new evidence for the endocrinology of Hatschek's pit and for the origin and evolution of vertebrate adenohypophysis.

  19. Gene regulation in amphioxus: An insight from transgenic studies in amphioxus and vertebrates.

    Science.gov (United States)

    Kozmikova, Iryna; Kozmik, Zbynek

    2015-12-01

    Cephalochordates, commonly known as amphioxus or lancelets, are the most basal subphylum of chordates. Cephalochordates are thus key to understanding the origin of vertebrates and molecular mechanisms underlying vertebrate evolution. The evolution of developmental control mechanisms during invertebrate-to-vertebrate transition involved not only gene duplication events, but also specific changes in spatial and temporal expression of many genes. To get insight into the spatiotemporal regulation of gene expression during invertebrate-to-vertebrate transition, functional studies of amphioxus gene regulatory elements are highly warranted. Here, we review transgenic studies performed in amphioxus and vertebrates using promoters and enhancers derived from the genome of Branchiostoma floridae. We describe the current methods of transgenesis in amphioxus, provide evidence of Tol2 transposon-generated transgenic embryos of Branchiostoma lanceolatum and discuss possible future directions. We envision that comparative transgenic analysis of gene regulatory sequences in the context of amphioxus and vertebrate embryos will likely provide an important mechanistic insight into the evolution of vertebrate body plan.

  20. Expression, purification and polyclonal antibody generation of p23,an Hsp90 cochaperone, in the amphioxus Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    ZHAO Bosheng; ZHANG Shicui; PANG Qiuxiang; LIU Zhenhui; LIANG Yujun

    2006-01-01

    The cDNA of amphioxus p23, a highly conserved co-chaperone for Hsp90, was cloned into a bacterial expression vector pGEX-6P-1 and the GST-tagged fusion protein was produced in Eschherichia coli cells. The recombinant p23 was purified by affinity purification, and its molecular mass was estimated to be approximately 22 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The N-terminus of purified p23 was sequenced, and the resulting amino acid sequence matches exactly the predicted residues deduced from the amphioxus p23 gene. Besides, polyclonal antibodies against the recombinant p23 were generated, and these antibodies not only recognized specifically the fusion protein GST-p23 from induced E. coli cells, purified GST-p23 and p23 protein, but also reacted with the total protein extracted from the adult amphioxus and formed a single positive band. These results pave the way for identifying its tissue and subcellular localization, and may open the door to clarifying its structure and mechanisms of biological role.

  1. Localization and expression of LHβmRNA in the brain and Hatschek's pit of amphioxus, Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    方永强; 周寿康; 翁幼竹; 赵瑞钦

    2001-01-01

    To determine whether gonadotropin-like substance in the brain and Hatschek' s pit of amphioxus issynthesized by the tissue in situ or transported from other tissue, a histochemical study was carried out by in situ hy- bridization using digoxigenin (DIG)-labelled LHβRNAprobes. The results showed that LHβmRNA expressed in the nerve cells of brain and the epithelial cells of Hatschek' s pit, thus providing new evidence for the homology of pituitary of ver- tebrates with Hatschek' s pit and the functional evolution of gonadotropin.

  2. A family of GFP-like proteins with different spectral properties in lancelet Branchiostoma floridae

    Directory of Open Access Journals (Sweden)

    Mushegian Arcady

    2008-07-01

    Full Text Available Abstract Background Members of the green fluorescent protein (GFP family share sequence similarity and the 11-stranded β-barrel fold. Fluorescence or bright coloration, observed in many members of this family, is enabled by the intrinsic properties of the polypeptide chain itself, without the requirement for cofactors. Amino acid sequence of fluorescent proteins can be altered by genetic engineering to produce variants with different spectral properties, suitable for direct visualization of molecular and cellular processes. Naturally occurring GFP-like proteins include fluorescent proteins from cnidarians of the Hydrozoa and Anthozoa classes, and from copepods of the Pontellidae family, as well as non-fluorescent proteins from Anthozoa. Recently, an mRNA encoding a fluorescent GFP-like protein AmphiGFP, related to GFP from Pontellidae, has been isolated from the lancelet Branchiostoma floridae, a cephalochordate (Deheyn et al., Biol Bull, 2007 213:95. Results We report that the nearly-completely sequenced genome of Branchiostoma floridae encodes at least 12 GFP-like proteins. The evidence for expression of six of these genes can be found in the EST databases. Phylogenetic analysis suggests that a gene encoding a GFP-like protein was present in the common ancestor of Cnidaria and Bilateria. We synthesized and expressed two of the lancelet GFP-like proteins in mammalian cells and in bacteria. One protein, which we called LanFP1, exhibits bright green fluorescence in both systems. The other protein, LanFP2, is identical to AmphiGFP in amino acid sequence and is moderately fluorescent. Live imaging of the adult animals revealed bright green fluorescence at the anterior end and in the basal region of the oral cirri, as well as weaker green signals throughout the body of the animal. In addition, red fluorescence was observed in oral cirri, extending to the tips. Conclusion GFP-like proteins may have been present in the primitive Metazoa. Their

  3. Discovery of germline-related genes in Cephalochordate amphioxus: A genome wide survey using genome annotation and transcriptome data.

    Science.gov (United States)

    Yue, Jia-Xing; Li, Kun-Lung; Yu, Jr-Kai

    2015-12-01

    The generation of germline cells is a critical process in the reproduction of multicellular organisms. Studies in animal models have identified a common repertoire of genes that play essential roles in primordial germ cell (PGC) formation. However, comparative studies also indicate that the timing and regulation of this core genetic program vary considerably in different animals, raising the intriguing questions regarding the evolution of PGC developmental mechanisms in metazoans. Cephalochordates (commonly called amphioxus or lancelets) represent one of the invertebrate chordate groups and can provide important information about the evolution of developmental mechanisms in the chordate lineage. In this study, we used genome and transcriptome data to identify germline-related genes in two distantly related cephalochordate species, Branchiostoma floridae and Asymmetron lucayanum. Branchiostoma and Asymmetron diverged more than 120 MYA, and the most conspicuous difference between them is their gonadal morphology. We used important germline developmental genes in several model animals to search the amphioxus genome and transcriptome dataset for conserved homologs. We also annotated the assembled transcriptome data using Gene Ontology (GO) terms to facilitate the discovery of putative genes associated with germ cell development and reproductive functions in amphioxus. We further confirmed the expression of 14 genes in developing oocytes or mature eggs using whole mount in situ hybridization, suggesting their potential functions in amphioxus germ cell development. The results of this global survey provide a useful resource for testing potential functions of candidate germline-related genes in cephalochordates and for investigating differences in gonad developmental mechanisms between Branchiostoma and Asymmetron species.

  4. The evolution of genes encoding for green fluorescent proteins: insights from cephalochordates (amphioxus)

    Science.gov (United States)

    Yue, Jia-Xing; Holland, Nicholas D.; Holland, Linda Z.; Deheyn, Dimitri D.

    2016-06-01

    Green Fluorescent Protein (GFP) was originally found in cnidarians, and later in copepods and cephalochordates (amphioxus) (Branchiostoma spp). Here, we looked for GFP-encoding genes in Asymmetron, an early-diverged cephalochordate lineage, and found two such genes closely related to some of the Branchiostoma GFPs. Dim fluorescence was found throughout the body in adults of Asymmetron lucayanum, and, as in Branchiostoma floridae, was especially intense in the ripe ovaries. Spectra of the fluorescence were similar between Asymmetron and Branchiostoma. Lineage-specific expansion of GFP-encoding genes in the genus Branchiostoma was observed, largely driven by tandem duplications. Despite such expansion, purifying selection has strongly shaped the evolution of GFP-encoding genes in cephalochordates, with apparent relaxation for highly duplicated clades. All cephalochordate GFP-encoding genes are quite different from those of copepods and cnidarians. Thus, the ancestral cephalochordates probably had GFP, but since GFP appears to be lacking in more early-diverged deuterostomes (echinoderms, hemichordates), it is uncertain whether the ancestral cephalochordates (i.e. the common ancestor of Asymmetron and Branchiostoma) acquired GFP by horizontal gene transfer (HGT) from copepods or cnidarians or inherited it from the common ancestor of copepods and deuterostomes, i.e. the ancestral bilaterians.

  5. Expression of Fox genes in the cephalochordate Branchiostoma lanceolatum

    Directory of Open Access Journals (Sweden)

    Daniel eAldea

    2015-07-01

    Full Text Available Forkhead box (Fox genes code for transcription factors that play important roles in different biological processes. They are found in a wide variety of organisms and appeared in unicellular eukaryotes. In metazoans, the gene family includes many members that can be subdivided into 24 classes. Cephalochordates are key organisms to understand the functional evolution of gene families in the chordate lineage due to their phylogenetic position as an early divergent chordate, their simple anatomy and genome structure. In the genome of the cephalochordate amphioxus Branchiostoma floridae, 32 Fox genes were identified, with at least one member for each of the classes that were present in the ancestor of bilaterians. In this work we describe the expression pattern of 13 of these genes during the embryonic development of the Mediterranean amphioxus, Branchiostoma lanceolatum. We found that FoxK and FoxM genes present an ubiquitous expression while all the others show specific expression patterns restricted to diverse embryonic territories. Many of these expression patterns are conserved with vertebrates, suggesting that the main functions of Fox genes in chordates were present in their common ancestor.

  6. Immunolocalization of aromatase, estrogen and estrogen receptor α and β in the epithelium of digestive tract and enteric neurons of amphioxus (Branchiostoma belcheri )

    Institute of Scientific and Technical Information of China (English)

    FANG Yongqiang; WENG Youzhu; YE Rongzhong; LIU Lili

    2005-01-01

    Immunohistochemical localization of aromatase, estrogen and estrogen receptor in the digestive tract and enteric neurons of amphioxus is investigated. It was found that immunoreactive proteins of aromatase, estrogen and ER-α and β are expressed in hepatic diverticulum, epithelial cells of anterior and posterior region of midgut, as well as in enteric neurons, while hindgut showed immunonegative. The results suggest that digestive tract of amphioxus may be able to synthesize estrogen and possess endocrine function, like rat gastric epithelium and enteric neurons in mammals. The present study provides authentic morphological evidence for explaining the action mechanism of estrogen in regulating the digestive function of gut and the functional evolution of estrogen, which has important theoretical significance in amphioxus.

  7. Verification, Characterization and Tissue-specific Expression of UreG, a Urease Accessory Protein Gene, from the Amphioxus Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    Ji-Yu XUE; Shi-Cui ZHANG; Nai-Guo LIU; Zhen-Hui LIU

    2006-01-01

    UreG genes have been found in bacteria, fungi and plants but have not yet identified in animals,although a putative UreG-like gene has been documented in sea urchin. In the course of a large-scale sequencing of amphioxus gut cDNA library, we have identified a cDNA with high similarity to UreG genes. Both reverse transcription-polymerase chain reaction and nested polymerase chain reaction, as well as in situ hybridization histochemistry, verified that the cDNA represented an amphioxus UreG gene (AmphiUreG) rather than a microbial contaminant of the cDNA library. This is further supported by the presence of urease activity in amphioxus gut, gill and ovary. AmphiUreG encodes a deduced protein of 200 amino acid residues including a highly conserved P-loop, beating approximately 46%-49%, 44%-48%, and 29%-37% similarity to fungal,plant and bacterial UreG proteins, respectively. It shows a tissue-specific expression pattern in amphioxus,and is especially abundant in the digestive system. This is the first UreG gene identified in animal species.

  8. Distribution of growth hormone-like immunoreactive cells and somatostatin receptors in the nervous system and Hatschek's pit of amphioxus, Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Using immunohistochemical method and double staining technique, the localization of growth hormone (GH) and somatostatin receptors in the nervous system and Hatschek's pit of amphioxus has been investigated. The results showed that the growth hormone-like nerve cells and endocrine cells as well as three subtypes-of somatostatin receptors exist in the nervous system and Hatschek's pit, and GH-like nerve cells and endocrine cells co-exist with three subtypes of somatostatin receptors in the brain vesicle and Hatschek's pit. It is suggested that a primitive control system of inhibitory growth hormone secretion in Hatschek's pit could have been developed in amphioxus, as in vertebrates. The present study provides new evidence for the endocrinology and the evolution of Hatschek's pit.

  9. The amphioxus genome and the evolution of the chordate karyotype

    Energy Technology Data Exchange (ETDEWEB)

    Putnam, Nicholas H.; Butts, Thomas; Ferrier, David E.K.; Furlong, Rebecca F.; Hellsten, Uffe; Kawashima, Takeshi; Robinson-Rechavi, Marc; Shoguchi, Eiichi; Terry, Astrid; Yu, Jr-Kai; Benito-Gutierrez, Elia; Dubchak, Inna; Garcia-Fernandez, Jordi; Gibson-Brown, Jeremy J.; Grigoriev, Igor V.; Horton, Amy C.; de Jong, Pieter J.; Jurka, Jerzy; Kapitonov, Vladimir; Kohara, Yuji; Kuroki, Yoko; Lindquist, Erika; Lucas, Susan; Osoegawa, Kazutoyo; Pennacchio, Len A.; Salamov, Asaf A.; Satou, Yutaka; Sauka-Spengler, Tatjana; Schmutz[, Jeremy; Shin-I, Tadasu; Toyoda, Atsushi; Bronner-Fraser, Marianne; Fujiyama, Asao; Holland, Linda Z.; Holland, Peter W. H.; Satoh, Nori; Rokhsar, Daniel S.

    2008-04-01

    Lancelets ('amphioxus') are the modern survivors of an ancient chordate lineage with a fossil record dating back to the Cambrian. We describe the structure and gene content of the highly polymorphic {approx}520 million base pair genome of the Florida lancelet Branchiostoma floridae, and analyze it in the context of chordate evolution. Whole genome comparisons illuminate the murky relationships among the three chordate groups (tunicates, lancelets, and vertebrates), and allow reconstruction of not only the gene complement of the last common chordate ancestor, but also a partial reconstruction of its genomic organization, as well as a description of two genome-wide duplications and subsequent reorganizations in the vertebrate lineage. These genome-scale events shaped the vertebrate genome and provided additional genetic variation for exploitation during vertebrate evolution.

  10. Distribution of Alkline Phosphatase in Amphioxus Branchiostoma belcheri tsingtaunese%碱性磷酸酶在文昌鱼体内的分布

    Institute of Scientific and Technical Information of China (English)

    王锐; 张士璀

    2001-01-01

    The distribution of alkaline phosphatase in amphioxus has not yetbeen reported, and was thus investigated in the present study. It is found that the enzyme is present in the metapleural fold epidermis, atrial epithelium, mesothelium enclosing the dorsal fin box, brush border of mid-gut lumen and caecum lumen, genital epithelium, dividing spermatogonia and the connectivetis sueenveloping the somatic muscles.%采用组化方法首次探讨碱性磷酸酶在文昌鱼体内的分布。结果发现:碱性磷酸酶存在于腹褶部分表皮、围腮腔上皮、构成背鳍腔的内皮、肌膜、中肠腔和肝盲囊腔细胞纹状缘、生殖上皮和精原细胞中。

  11. No more than 14: the end of the amphioxus Hox cluster

    Directory of Open Access Journals (Sweden)

    2005-01-01

    Full Text Available The Hox gene cluster has been a key paradigm for a generation of developmental and evolutionary biologists. Since its discovery in the mid-1980's, the identification, genomic organization, expression, colinearity, and regulation of Hox genes have been immediate targets for study in any new model organism, and metazoan genome projects always refer to the structure of the particular Hox cluster(s. Since the early 1990's, it has been dogma that vertebrate Hox clusters are composed of thirteen paralogous groups. Nonetheless, we showed that in the otherwise prototypical cephalochordate amphioxus (Branchiostoma floridae, the Hox cluster contains a fourteenth Hox gene, and very recently, a 14th Hox paralogous group has been found in the coelacanth and the horn shark, suggesting that the amphioxus cluster was anticipating the finding of Hox 14 in some vertebrate lineages. In view of the pivotal place that amphioxus occupies in vertebrate evolution, we thought it of considerable interest to establish the limits of its Hox gene cluster, namely resolution of whether more Hox genes are present in the amphioxus cluster (e.g., Hox 15. Using two strategies, here we report the completion and characterization of the Hox gene content of the single amphioxus Hox cluster, which encompasses 650 kb from Hox1 to Evx. Our data have important implications for the primordial Hox gene cluster of chordates: the prototypical nature of the single amphioxus Hox cluster makes it unlikely that additional paralogous groups will be found in any chordate lineage. We suggest that 14 is the end.

  12. 厦门国家级自然保护区厦门文昌鱼资源及其保护%RESOURCES AND PROTETION OF AMPHIOXUS (Branchiostoma belcheri)IN XIAMEN NATIONAL NATURAL RESERVE AREA OF RARE MARINE SPECIES

    Institute of Scientific and Technical Information of China (English)

    方少华; 吕小梅; 张跃平

    2002-01-01

    根据 2001年 4月~ 2002年 3月在厦门文昌鱼 (Branchiostoma belcheri)自然保护区进行 4个季度月的文昌鱼资源调查资料,估算保护区内文昌鱼现有资源量约有 27.6 t,分析了影响文昌鱼生存的不利因素,并对保护区管理与保护提出建议.

  13. Interplay Between Amphioxus Complement with Sea Bass Macrophages: Opsonic Activity of Amphioxus Humoral Fluids

    Institute of Scientific and Technical Information of China (English)

    PAN Junli; LIU Min; ZHANG Shicui

    2011-01-01

    Previous studies have shown the existence of a complement system in the amphioxus Branchiostoma japonicum.However,whether it has an opsonic activity similar to that of vertebrates remains unknown.We demonstrated that the humoral fluid (HF)of amphioxus promoted the phagocytosis of yeast cells with sea bass (Lateolabraxjaponicus) macrophages,whereas the C3-depleted and heated HF significantly lost the phagocytosis-promoting capacity.In addition,the precipitation of factor B (Bf) led to a marked loss of opsonic activity.Moreover,C3 fragments in the HF were found to bind to yeast cell surfaces.The results indicate that the amphioxus complement system is an important element involved in the opsonic activity,which promotes the sea bass macrophage phagocytosis by tagging yeast cells with C3 fragments via the activation of alternative complement pathway.

  14. Spatiotemporal expression of Pax genes in amphioxus: Insights into Pax-related organogenesis and evolution

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    The expression of four AmphiPax genes in 16 developmental stages and different organs in amphioxus (Branchiostoma belcheri) was investigated, finding those genes expressed throughout amphioxus life with temporal-specific (especially during embryogenesis and metamorphosis) and spatial-specific patterns. This study suggests that duplicated Pax genes in vertebrates might maintain most of their ancestral functions and also expand their expression patterns after the divergence of protochordates and vertebrates.

  15. Molecular cloning of amphioxus uncoupling protein and assessment of its uncoupling activity using a yeast heterologous expression system

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Kun [Jiangsu Diabetes Research Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu (China); Sun, Guoxun [Department of Hematology, Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001 (China); Lv, Zhiyuan; Wang, Chen [Jiangsu Diabetes Research Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu (China); Jiang, Xueyuan, E-mail: xueyuanjiang@yahoo.com.cn [Jiangsu Diabetes Research Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu (China); Li, Donghai, E-mail: lidonghai@gmail.com [Jiangsu Diabetes Research Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu (China); Zhang, Chenyu, E-mail: cyzhang@nju.edu.cn [Jiangsu Diabetes Research Center, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu (China)

    2010-10-01

    Research highlights: {yields} Invertebrates, for example amphioxus, do express uncoupling proteins. {yields} Both the sequence and the uncoupling activity of amphioxus UCP resemble UCP2. {yields} UCP1 is the only UCP that can form dimer on yeast mitochondria. -- Abstract: The present study describes the molecular cloning of a novel cDNA fragment from amphioxus (Branchiostoma belcheri) encoding a 343-amino acid protein that is highly homologous to human uncoupling proteins (UCP), this protein is therefore named amphioxus UCP. This amphioxus UCP shares more homology with and is phylogenetically more related to mammalian UCP2 as compared with UCP1. To further assess the functional similarity of amphioxus UCP to mammalian UCP1 and -2, the amphioxus UCP, rat UCP1, and human UCP2 were separately expressed in Saccharomyces cerevisiae, and the recombinant yeast mitochondria were isolated and assayed for the state 4 respiration rate and proton leak, using pYES2 empty vector as the control. UCP1 increased the state 4 respiration rate by 2.8-fold, and the uncoupling activity was strongly inhibited by GDP, while UCP2 and amphioxus UCP only increased the state 4 respiration rate by 1.5-fold and 1.7-fold in a GDP-insensitive manner, moreover, the proton leak kinetics of amphioxus UCP was very similar to UCP2, but much different from UCP1. In conclusion, the amphioxus UCP has a mild, unregulated uncoupling activity in the yeast system, which resembles mammalian UCP2, but not UCP1.

  16. Vertebrate-like regeneration in the invertebrate chordate amphioxus.

    Science.gov (United States)

    Somorjai, Ildikó M L; Somorjai, Rajmund L; Garcia-Fernàndez, Jordi; Escrivà, Hector

    2012-01-10

    An important question in biology is why some animals are able to regenerate, whereas others are not. The basal chordate amphioxus is uniquely positioned to address the evolution of regeneration. We report here the high regeneration potential of the European amphioxus Branchiostoma lanceolatum. Adults regenerate both anterior and posterior structures, including neural tube, notochord, fin, and muscle. Development of a classifier based on tail regeneration profiles predicts the assignment of young and old adults to their own class with >94% accuracy. The process involves loss of differentiated characteristics, formation of an msx-expressing blastema, and neurogenesis. Moreover, regeneration is linked to the activation of satellite-like Pax3/7 progenitor cells, the extent of which declines with size and age. Our results provide a framework for understanding the evolution and diversity of regeneration mechanisms in vertebrates.

  17. Expression of AmphiNaC, a new member of the amiloride-sensitive sodium channel related to degenerins and epithelial sodium channels in amphioxus

    Directory of Open Access Journals (Sweden)

    2006-04-01

    Full Text Available Degenerins and amiloride-sensitive Na+ channels form a new family of cationic ion channels (DEG/NaC. DEG/NaC family emerged as common denominator within a metazoan mechanosensory apparatus. In this study, we characterized a new member of such family in amphioxus, Branchiostoma floridae. The AmphiNaC cDNA sequence encodes a protein showing amino acid residues characteristic of DEG/NaC family, such as two hydrophobic domains surrounding a large extracellular loop that includes cystein-rich domains; nevertheless its predicted sequence is quite divergent from other family members. AmphiNaC is expressed at early larval stage in some putative sensory epidermal cells in the middle of the body and in neurons of the posterior cerebral vesicle, as well as in some ventrolateral and mediolateral neurons of the neural tube. In late larvae, AmphiNaC expression is maintained in some neurons of the neural tube, and it is expressed in putative sensory epidermal cells of rostrum and mouth. The analysis of AmphiNaC gene expression pattern suggests that it might be involved in neurotransmission and sensory modulation.

  18. An amphioxus orthologue of the estrogen receptor that does not bind estradiol: Insights into estrogen receptor evolution

    Directory of Open Access Journals (Sweden)

    Laudet Vincent

    2008-07-01

    Full Text Available Abstract Background The origin of nuclear receptors (NRs and the question whether the ancestral NR was a liganded or an unliganded transcription factor has been recently debated. To obtain insight into the evolution of the ligand binding ability of estrogen receptors (ER, we comparatively characterized the ER from the protochordate amphioxus (Branchiostoma floridae, and the ER from lamprey (Petromyzon marinus, a basal vertebrate. Results Extensive phylogenetic studies as well as signature analysis allowed us to confirm that the amphioxus ER (amphiER and the lamprey ER (lampER belong to the ER group. LampER behaves as a "classical" vertebrate ER, as it binds to specific DNA Estrogen Responsive Elements (EREs, and is activated by estradiol (E2, the classical ER natural ligand. In contrast, we found that although amphiER binds EREs, it is unable to bind E2 and to activate transcription in response to E2. Among the 7 natural and synthetic ER ligands tested as well as a large repertoire of 14 cholesterol derivatives, only Bisphenol A (an endocrine disruptor with estrogenic activity bound to amphiER, suggesting that a ligand binding pocket exists within the receptor. Parsimony analysis considering all available ER sequences suggest that the ancestral ER was not able to bind E2 and that this ability evolved specifically in the vertebrate lineage. This result does not support a previous analysis based on ancestral sequence reconstruction that proposed the ancestral steroid receptor to bind estradiol. We show that biased taxonomic sampling can alter the calculation of ancestral sequence and that the previous result might stem from a high proportion of vertebrate ERs in the dataset used to compute the ancestral sequence. Conclusion Taken together, our results highlight the importance of comparative experimental approaches vs ancestral reconstructions for the evolutionary study of endocrine systems: comparative analysis of extant ERs suggests that the

  19. The cult of amphioxus in German Darwinism; or, our gelatinous ancestors in Naples' blue and balmy bay.

    Science.gov (United States)

    Hopwood, Nick

    2015-01-01

    Biologists having rediscovered amphioxus, also known as the lancelet or Branchiostoma, it is time to reassess its place in early Darwinist debates over vertebrate origins. While the advent of the ascidian-amphioxus theory and challenges from various competitors have been, documented, this article offers a richer account of the public appeal of amphioxus as a primitive ancestor. The focus is on how the 'German Darwin' Ernst Haeckel persuaded general magazine and newspaper readers to revere this "flesh of our flesh and blood of our blood", and especially on Das neue Laienbrevier des Haeckelismus (The new lay breviary of Haeckelism) by Moritz Reymond with cartoons by Fritz Steub. From the late 1870s these successful little books of verse introduced the Neapolitan discoveries that made the animal's name and satirized Haeckel's rise as high priest of its cult. One song is reproduced and translated here, with a contemporary "imitation" by the Canadian palaeontologist Edward John Chapman, and extracts from others. Predating the American "It's a long way from amphioxus" by decades, these rhymes dramatize neglected 'species politics' of Darwinism and highlight the roles of humour in negotiating evolution.

  20. Comment: 62 [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available Florida lancelet (amphioxus) Branchiostoma floridae Branchiostoma_floridae_L.png イメージを差し替えました (イメージの天地が逆でした) ttamura 2009/04/29 21:28:13 ...

  1. Effects of damage of Hatschek's pit of amphioxus on its structure and function

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    It is found for the first time that, after the structure and endocrine function of Hatschek's pit of Branchiostoma belcheri are damaged by monosodium glutamate (MSG) treatment in breeding season, the ovulation of mature ovary, as well as the development of both ovary and testis of large-growth stage are obstructed and stagnated;and in non-breeding season, the process of gonadal development is delayed. The disturbed ovulation and gonadal development can definitely be restored with exogenous gonadotropic hormone as the therapeutical substitute. These results may provide new proof for the complete understanding of the evolutional process of pituitary function in vertebrates and playing an important role in the reproductive endocrine control system of Hatschek's pit of amphioxus.

  2. Characterization and expression of a cDNA, AmphiSDHD,encoding the amphioxus cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase

    Institute of Scientific and Technical Information of China (English)

    MA Lifang; ZHANG Shicui; ZHUANG Zhimeng; LIU Zhenhui; LI Hongyan; XIA Jianjun

    2005-01-01

    In this study, an amphioxus cDNA, AmphiSDHD, encoding the cytochrome b small subunit in mitochondrial succinate-ubiquinone oxidoreductase, was isolated from the gut cDNA library of amphioxus Branchiostoma belcheri tsingtauense. It is 1429 bp in length, with an open reading frame of 465 bp coding for a protein of 154 amino acids. The deduced protein contains a mitochondrial targeting presequence of 65 amino acids rich in basic residues like arginine and hydroxy residues such as serine and threonine. Alignment of the amino acid sequences of AmphiSDHD and other eukaryotic SDHD proteins showed that AmphiSDHD has three transmembrane segments, and includes two histidine residues in the second transmembrane segment that are the putative binding sites for the heme b molecule. The phylogenetic tree constructed suggests that AmphiSDHD appears more closely related to vertebrate SDHD proteins than invertebrate ones. Northern blotting demonstrated that AmphiSDHD is ubiquitously expressed in amphioxus, being in line with the fact that SDHD is a house-keeping protein.

  3. Developmental expression of amphioxus RACK1

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Vertebrate RACK1 plays a key role in embryonic development. This paper described the cloning, phy- logenetic analysis and developmental expression of AmphiRACK1, the RACK1 homologous gene in amphioxus. Phylogenetic analysis indicated that amphioxus RACK1 was located at the base of verte- brate clade. AmphiRACK1 expression in lithium-treated embryos was also examined. During embryonic development, AmphiRACK1 was expressed strongly in cerebral vesicles, neural tubes and somites. In lithium-treated embryos, the segmental expression of AmphiRACK1 in somites became blurry and decreased. Its expression in cerebral vesicles and neural tubes was also weaker or disappeared. In the adult animal, AmphiRACK1 transcripts were detected in the epithelium of midgut diverticulus and gut, wheel organ, gill blood vessels and testis.

  4. Developmental expression of amphioxus RACK1

    Institute of Scientific and Technical Information of China (English)

    HUANG XiangWei; ZHANG Wei; LI XinYi; ZHANG XiaoHui; LI BaoJun; MAO BingYu; ZHANG HongWei

    2007-01-01

    Vertebrate RACK1 plays a key role in embryonic development. This paper described the cloning, phyIogenetic analysis and developmental expression of AmphiRACK1, the RACK1 homologous gene in amphioxus. Phylogenetic analysis indicated that amphioxus RACK1 was located at the base of vertebrate clade. AmphiRACK1 expression in lithium-treated embryos was also examined. During embryonic development, AmphiRACK1 was expressed strongly in cerebral vesicles, neural tubes and somites. In lithium-treated embryos, the segmental expression of AmphiRACK1 in somites became blurry and decreased. Its expression in cerebral vesicles and neural tubes was also weaker or disappeared. In the adult animal, AmphiRACK1 transcripts were detected in the epithelium of midgut diverticulus and gut,wheel organ, gill blood vessels and testis.

  5. 补体和溶菌酶在清除文昌鱼体内细菌中的作用%Actions of Complement and Lysozyme in Clearance of Bacterium Escherichia coli in Amphioxus

    Institute of Scientific and Technical Information of China (English)

    王光锋; 张士璀

    2013-01-01

    It remains unknown to date about the mechanism that pathogens are eliminated from amphioxus (Branchiostoma belcheri) lacking free circulating blood cells even though amphioxus is widely used as the model organism for evolution and development research .The bacterium clearance dynamics in amphioxus were studied in vivo and in vitro by lysis of Escherichia coli ,inhibition of lytic activity with the specific antibodies ,and western blot .It was found that the amphioxus was capable of efficiently eliminating the invading bacterium Escherichia coli and that the humoral fluids can readily lyse the bacterium in v itro . Both complement and lysozyme were shown to be involved in the elimination in vivo of the invading bacteria in amphioxus and to act in concert against the invading bacteria , the complement playing a stronger role than the lysozyme doing . Western blot showed that both C3α and lysozyme levels were increased with the challenge with E .coli .This is the first report addressing the factors responsible for the clearance in v ivo of invading bacteria in amphioxus .%文昌鱼作为研究脊椎动物起源和进化的模式生物,由于缺乏自由循环的血细胞,关于其清除体内病原体的机制,迄今尚无报道。采用体内细菌清除试验、体外杀菌试验、特异性抗体抑制试验、抑制补体途径试验及Western blot方法,研究了文昌鱼体内细菌清除机制及动力学。试验结果表明,文昌鱼能有效清除入侵的异源微生物,体液对大肠杆菌具有较强的杀灭活性。补体和溶菌酶是参与清除入侵大肠杆菌的主要因子,通过替代途径激活的补体较溶菌酶作用更强,这是关于文昌鱼清除体内细菌机制及其相关因子的首次报道。

  6. Protochordate amphioxus is an emerging model organism for comparative immunology

    Institute of Scientific and Technical Information of China (English)

    Shicui Zhang; Yujun Liang; Guangdong Jia; Zhimeng Zhuang

    2009-01-01

    Protochordate amphioxus is an extant invertebrate regarded quite recently as a basal chordate. It has a vertebrate-like body plan including a circulation system with an organization similar to that of vertebrates. However, amphioxus is less complex than vertebrates for having a genome uncomplicated by extensive genomic duplication, and lacking lymphoid organs and free circulating blood cells.Recent studies on immunity have demonstrated the presence in amphioxus of both the constituent elements of key molecules involved in adaptive immunity such as proto-major histocompatibility complex (proto-MHC), V region-containing chitin-binding protein (VCBP)and V and C domain-bearing protein (VCP), and the complement system operating via the alternative and lectin pathways resembling those seen in vertebrates. In addition, the acute phase response profile in amphioxus has been shown to be similar to that observed in vertebrates. These findings together with the relative structural and genomic simplicity make amphioxus an ideal organism for gaining insights into the origin and evolution of the vertebrate immune system, especially adaptive immunity, and the composition and mech-anisms of the vertebrate innate immunity.

  7. Hormonal Regulation of Oocyte Development and Maturation of Amphioxus

    Institute of Scientific and Technical Information of China (English)

    方永强; 赵维信; 林秋明

    1994-01-01

    Using the electron microscopic technique and radioimmunoassay,the hormonal regulationof the oocyte development and maturation of the amphioxus has been investigated.The results indicate thatthe exogenous GnRH-A can stimulate the oocyte development and maturation as well as the spawning of theamphioxus.It has further been revealed by radioimmunoassay that GnRH-A can increase the content of sexsteroid hormone,especially,estradiol-17β,by 1—3 times.The increase of estradiol-17β coordinates withthe synthesis of oocyte into yolk substance.The results further justify that the hormonal regulation in theoocyte development and maturation of amphioxus is similar to that of vertebrates.This is of great theoreticalsignificance for reproduetive endocrine physiology of amphioxus.

  8. The single amphioxus Mox gene: insights into the functional evolution of Mox genes, somites, and the asymmetry of amphioxus somitogenesis.

    Science.gov (United States)

    Minguillón, Carolina; Garcia-Fernàndez, Jordi

    2002-06-15

    Mox genes are members of the "extended" Hox-cluster group of Antennapedia-like homeobox genes. Homologues have been cloned from both invertebrate and vertebrate species, and are expressed in mesodermal tissues. In vertebrates, Mox1 and Mox2 are distinctly expressed during the formation of somites and differentiation of their derivatives. Somites are a distinguishing feature uniquely shared by cephalochordates and vertebrates. Here, we report the cloning and expression of the single amphioxus Mox gene. AmphiMox is expressed in the presomitic mesoderm (PSM) during early amphioxus somitogenesis and in nascent somites from the tail bud during the late phase. Once a somite is completely formed, AmphiMox is rapidly downregulated. We discuss the presence and extent of the PSM in both phases of amphioxus somitogenesis. We also propose a scenario for the functional evolution of Mox genes within chordates, in which Mox was co-opted for somite formation before the cephalochordate-vertebrate split. Novel expression sites found in vertebrates after somite formation postdated Mox duplication in the vertebrate stem lineage, and may be linked to the increase in complexity of vertebrate somites and their derivatives, e.g., the vertebrae. Furthermore, AmphiMox expression adds new data into a long-standing debate on the extent of the asymmetry of amphioxus somitogenesis.

  9. Insights from amphioxus into the evolution of vertebrate cartilage.

    Directory of Open Access Journals (Sweden)

    Daniel Meulemans

    Full Text Available Central to the story of vertebrate evolution is the origin of the vertebrate head, a problem difficult to approach using paleontology and comparative morphology due to a lack of unambiguous intermediate forms. Embryologically, much of the vertebrate head is derived from two ectodermal tissues, the neural crest and cranial placodes. Recent work in protochordates suggests the first chordates possessed migratory neural tube cells with some features of neural crest cells. However, it is unclear how and when these cells acquired the ability to form cellular cartilage, a cell type unique to vertebrates. It has been variously proposed that the neural crest acquired chondrogenic ability by recruiting proto-chondrogenic gene programs deployed in the neural tube, pharynx, and notochord. To test these hypotheses we examined the expression of 11 amphioxus orthologs of genes involved in neural crest chondrogenesis. Consistent with cellular cartilage as a vertebrate novelty, we find that no single amphioxus tissue co-expresses all or most of these genes. However, most are variously co-expressed in mesodermal derivatives. Our results suggest that neural crest-derived cartilage evolved by serial cooption of genes which functioned primitively in mesoderm.

  10. The amphioxus genome provides unique insight into the evolution of immunity.

    Science.gov (United States)

    Dishaw, Larry J; Haire, Robert N; Litman, Gary W

    2012-03-01

    Immune systems evolve as essential strategies to maintain homeostasis with the environment, prevent microbial assault and recycle damaged host tissues. The immune system is composed of two components, innate and adaptive immunity. The former is common to all animals while the latter consists of a vertebrate-specific system that relies on somatically derived lymphocytes and is associated with near limitless genetic diversity as well as long-term memory. Deuterostome invertebrates provide a view of immune repertoires in phyla that immediately predate the origins of vertebrates. Genomic studies in amphioxus, a cephalochordate, have revealed homologs of genes encoding most innate immune receptors found in vertebrates; however, many of the gene families have undergone dramatic expansions, greatly increasing the innate immune repertoire. In addition, domain-swapping accounts for the innovation of new predicted pathways of receptor function. In both amphioxus and Ciona, a urochordate, the VCBPs (variable region containing chitin-binding proteins), which consist of immunoglobulin V (variable) and chitin binding domains, mediate recognition through the V domains. The V domains of VCBPs in amphioxus exhibit high levels of allelic complexity that presumably relate to functional specificity. Various features of the amphioxus immune repertoire reflect novel selective pressures, which likely have resulted in innovative strategies. Functional genomic studies underscore the value of amphioxus as a model for studying innate immunity and may help reveal how unique relationships between innate immune receptors and both pathogens and symbionts factored in the evolution of adaptive immune systems.

  11. Id expression in amphioxus and lamprey highlights the role of gene cooption during neural crest evolution

    Science.gov (United States)

    Meulemans, Daniel; McCauley, David; Bronner-Fraser, Marianne

    2003-01-01

    Neural crest cells are unique to vertebrates and generate many of the adult structures that differentiate them from their closest invertebrate relatives, the cephalochordates. Id genes are robust markers of neural crest cells at all stages of development. We compared Id gene expression in amphioxus and lamprey to ask if cephalochordates deploy Id genes at the neural plate border and dorsal neural tube in a manner similar to vertebrates. Furthermore, we examined whether Id expression in these cells is a basal vertebrate trait or a derived feature of gnathostomes. We found that while expression of Id genes in the mesoderm and endoderm is conserved between amphioxus and vertebrates, expression in the lateral neural plate border and dorsal neural tube is a vertebrate novelty. Furthermore, expression of lamprey Id implies that recruitment of Id genes to these cells occurred very early in the vertebrate lineage. Based on expression in amphioxus we postulate that Id cooption conferred sensory cell progenitor-like properties upon the lateral neurectoderm, and pharyngeal mesoderm-like properties upon cranial neural crest. Amphioxus Id expression is also consistent with homology between the anterior neurectoderm of amphioxus and the presumptive placodal ectoderm of vertebrates. These observations support the idea that neural crest evolution was driven in large part by cooption of multipurpose transcriptional regulators from other tissues and cell types.

  12. Metal dealing at the origin of the Chordata phylum: the metallothionein system and metal overload response in amphioxus.

    Directory of Open Access Journals (Sweden)

    Maria Guirola

    Full Text Available Non-vertebrate chordates, specifically amphioxus, are considered of the utmost interest for gaining insight into the evolutionary trends, i.e. differentiation and specialization, of gene/protein systems. In this work, MTs (metallothioneins, the most important metal binding proteins, are characterized for the first time in the cephalochordate subphylum at both gene and protein level, together with the main features defining the amphioxus response to cadmium and copper overload. Two MT genes (BfMT1 and BfMT2 have been identified in a contiguous region of the genome, as well as several ARE (antioxidant response element and MRE (metal response element located upstream the transcribed region. Their corresponding cDNAs exhibit identical sequence in the two lancelet species (B. floridae and B. lanceolatum, BfMT2 cDNA resulting from an alternative splicing event. BfMT1 is a polyvalent metal binding peptide that coordinates any of the studied metal ions (Zn, Cd or Cu rendering complexes stable enough to last in physiological environments, which is fully concordant with the constitutive expression of its gene, and therefore, with a metal homeostasis housekeeping role. On the contrary, BfMT2 exhibits a clear ability to coordinate Cd(II ions, while it is absolutely unable to fold into stable Cu (I complexes, even as mixed species. This identifies it as an essential detoxification agent, which is consequently only induced in emergency situations. The cephalochordate MTs are not directly related to vertebrate MTs, neither by gene structure, protein similarity nor metal-binding behavior of the encoded peptides. The closest relative is the echinoderm MT, which confirm proposed phylogenetic relationships between these two groups. The current findings support the existence in most organisms of two types of MTs as for their metal binding preferences, devoted to different biological functions: multivalent MTs for housekeeping roles, and specialized MTs that evolve

  13. Activities of Amphioxus GH-Like Protein in Osmoregulation: Insight into Origin of Vertebrate GH Family

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    Li, Mengyang; Jiang, Chengyan

    2017-01-01

    GH is known to play an important role in both growth promotion and osmoregulation in vertebrates. We have shown that amphioxus possesses a single GH-like hormone (GHl) gene encoding a functional protein capable of promoting growth. However, if GHl can mediate osmoregulation remains open. Here, we demonstrated clearly that GHl increased not only the survival rate of amphioxus but also the muscle moisture under high salinity. Moreover, GHl induced the expression of both the ion transporter Na+-K+-ATPase (NKA) and Na+-K+-2Cl− cotransporter (NKCC) in the gill as well as the mediator of GH action IGFl in the hepatic caecum, indicating that GHl fulfills this osmoregulatory activity through the same mechanisms of vertebrate GH. These results together suggest that the osmoregulatory activities of GH had emerged in the basal chordate amphioxus. We also proposed a new model depicting the origin of pituitary hormone family in vertebrates.

  14. On Branchiostoma californiense (Cephalochordata) from the Gulf of Nicoya estuary, Costa Rica.

    Science.gov (United States)

    Vargas, José A; Dean, Harlan K

    2010-12-01

    The cephalochordates are represented by the lancelets, of which species of the genus Branchiostoma are the best known. In recent years, these organisms have been the center of activity of studies focusing on the phylogenetic relationships of the chordates. In 1980, a survey of the benthos at 48 stations in the Gulf of Nicoya estuary, Pacific coast of Costa Rica, yielded 265 specimens of the lancelet Branchiostoma californiense. A total of 48 specimens was also collected at an intertidal flat in the mid upper estuary. Of the 48 subtidal stations, only eight had B. californiense, and these sites all had a sand fraction above 72%. The remaining stations ranged in their sand content from as low as 1% to as high as 92%, with an average of 25.9%, with 29 stations having a sand content lower than 72%. Lower salinities and muddy sediments may limit the distribution of the lancelet further upstream. This information is useful when changes over decades in the ecology of the estuary need to be evaluated against the background of local, regional, and global dynamics.

  15. Evolution of neural crest and placodes: amphioxus as a model for the ancestral vertebrate?

    Science.gov (United States)

    Holland, L. Z.; Holland, N. D.

    2001-01-01

    Recent studies of protochordates (ascidian tunicates and amphioxus) have given insights into possible ancestors of 2 of the characteristic features of the vertebrate head: neural crest and placodes. The neural crest probably evolved from cells on either side of the neural plate-epidermis boundary in a protochordate ancestral to the vertebrates. In amphioxus, homologues of several vertebrate neural crest marker genes (BMP2/4, Pax3/7, Msx, Dll and Snail) are expressed at the edges of the neural plate and/or adjacent nonneural ectoderm. Some of these markers are also similarly expressed in tunicates. In protochordates, however, these cells, unlike vertebrate neural crest, neither migrate as individuals through embryonic tissues nor differentiate into a wide spectrum of cell types. Therefore, while the protochordate ancestor of the vertebrates probably had the beginnings of a genetic programme for neural crest formation, this programme was augmented in the earliest vertebrates to attain definitive neural crest. Clear homologues of vertebrate placodes are lacking in protochordates. However, both amphioxus and tunicates have ectodermal sensory cells. In tunicates these are all primary neurons, sending axons to the central nervous system, while in amphioxus, the ectodermal sensory cells include both primary neurons and secondary neurons lacking axons. Comparisons of developmental gene expression suggest that the anterior ectoderm in amphioxus may be homologous to the vertebrate olfactory placode, the only vertebrate placode with primary, not secondary, neurons. Similarly, biochemical, morphological and gene expression data suggest that amphioxus and tunicates also have homologues of the adenohypophysis, one of the few vertebrate structures derived from nonneurogenic placodes. In contrast, the origin of the other vertebrate placodes is very uncertain.

  16. Ultrastructure and function of follicle cell in the ovary of Branchiostoma belcheri

    Institute of Scientific and Technical Information of China (English)

    Ulrich Welsch; 方永强

    1997-01-01

    The ultrastructure of follicle cells in the ovary at different developmental stages of Branchiostoma has been observed in detail with a transmission electron microscope. The results indicate that only one kind of follicle cell exists with structural features related to steroid hormone biosynthesis: (i) oval or round mitochondria with tubules; (ii) smooth surfaced endoplasmic reticulum; (iii) several large lipid droplets in the cytoplasm; (iv) a well de-veloped Golgi complex and tubular rough surfaced endoplasmic reticulum, as can be found in mammalian theca interna cells. In addition, as steroid hormone synthesizing cells, they obviously play an important role in the phagocytosis of relict gametes and cellular debris and may have a nutritive function for the oocytes. They can produce abundant secre-tory granules in stages III-IV ovaries. In mature ovaries they transform into flat epithelial cells with numerous micro-filaments which may play a role in ovulation.

  17. The effect of antibiotic exposure on eicosanoid generation from arachidonic acid and gene expression in a primitive chordate, Branchiostoma belcheri.

    Science.gov (United States)

    Yuan, Dongjuan; Pan, Minming; Zou, Qiuqiong; Chen, Chengyong; Chen, Shangwu; Xu, Anlong

    2015-01-01

    Chloramphenicol (Chl) is an effective antimicrobial agent widely used in veterinary medicine and commonly used in fish. Its use is restricted in the clinic because of adverse effects on the immune system and oxidative stress in mammals. However, the effects of Chl treatment on invertebrates remain unclear. Amphioxus, a basal chordate, is an ideal model to study the origin and evolution of the vertebrate immune system as it has a primary vertebrate-like arachidonic acid (AA) metabolic system. Here, we combined transcriptomic and lipidomic approaches to investigate the immune system and observe the oxygenated metabolites of AA to address the antibiotic effects on amphioxus. Tissue necrosis of the gill slits occurred in the Chl-treated amphioxus, but fewer epithelial cells were lost when treated with both Chl and ampicillin (Amp). The immune related pathways were dysregulated in both of the antibiotic treatment groups. The Chl alone treatment resulted in immunosuppression with down-regulation of the innate immune genes. In contrast, the Chl + Amp treatment resulted in immunostimulation to some extent, as shown by KEGG clustering. Furthermore, Chl induced a 3-fold reduction in the level of the eicosanoids, while the Chl + Amp treatment resulted in 1.7-fold increase of eicosanoid level. Thus in amphioxus, Amp might relieve the effects of the Chl-induced immune suppression and increase the level of eicosanoids from AA. Finally, the oxygenated metabolites from AA might be crucial to evaluate the effects of Chl treatment in animals.

  18. A study of neural-related microRNAs in the developing amphioxus

    Directory of Open Access Journals (Sweden)

    Candiani Simona

    2011-07-01

    Full Text Available Abstract Background MicroRNAs are small noncoding RNAs regulating expression of protein coding genes at post-transcriptional level and controlling several biological processes. At present microRNAs have been identified in various metazoans and seem also to be involved in brain development, neuronal differentiation and subtypes specification. An approach to better understand the role of microRNAs in animal gene expression is to determine temporal and tissue-specific expression patterns of microRNAs in different model organisms. Therefore, we have investigated the expression of six neural related microRNAs in amphioxus, an organism having an important phylogenetic position in terms of understanding the origin and evolution of chordates. Results In amphioxus, all the microRNAs we examined are expressed in specific regions of the CNS, and some of them are correlated with specific cell types. In addition, miR-7, miR-137 and miR-184 are also expressed in endodermal and mesodermal tissues. Several potential targets expressed in the nervous system of amphioxus have been identified by computational prediction and some of them are coexpressed with one or more miRNAs. Conclusion We identified six miRNAs that are expressed in the nervous system of amphioxus in a variety of patterns. miR-124 is found in both differentiating and mature neurons, miR-9 in differentiated neurons, miR-7, miR-137 and miR-184 in restricted CNS regions, and miR-183 in cells of sensory organs. Therefore, such amphioxus miRNAs may play important roles in regional patterning and/or specification of neuronal cell types.

  19. Anatomy of the Hesse photoreceptor cell axonal system in the central nervous system of amphioxus.

    Science.gov (United States)

    Castro, Antonio; Becerra, Manuela; Manso, María Jesús; Sherwood, Nancy M; Anadón, Ramón

    2006-01-01

    The present study reports the organization of the Hesse cell axonal system in the central nervous system of the amphioxus, with the use of a polyclonal antiserum raised against lamprey gonadotropin-releasing hormone-I (GnRH-I). In the spinal cord, the rhabdomeric photoreceptor cells of the bicellular organs were well labeled with this antibody. These cells sent smooth, straight, lateral processes that bent and became beaded as they passed ventrally and crossed to the contralateral side of the cord. There, the processes of several cells aggregated to give rise to a longitudinal fiber bundle. Beaded collaterals of these processes were directed to ventral neuropil and did not appear to contact giant Rohde cell axons. The crossed projections of the Hesse photoreceptors are compared with those of vertebrate retinal ganglion cells. Other antisera raised against GnRH weakly labeled rhabdomeric photoreceptors located dorsally in the brain, the Joseph cells. The finding that GnRH antibodies label amphioxus photoreceptor cells and axons is not definitive proof that the photoreceptors contain GnRH. Regardless of whether the antibody recognizes amphioxus GnRH, which has not yet been identified by structure, the antibody has revealed the processes of the Hesse photoreceptor cells.

  20. Melanopsin-expressing amphioxus photoreceptors transduce light via a phospholipase C signaling cascade.

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    Juan Manuel Angueyra

    Full Text Available Melanopsin, the receptor molecule that underlies light sensitivity in mammalian 'circadian' receptors, is homologous to invertebrate rhodopsins and has been proposed to operate via a similar signaling pathway. Its downstream effectors, however, remain elusive. Melanopsin also expresses in two distinct light-sensitive cell types in the neural tube of amphioxus. This organism is the most basal extant chordate and can help outline the evolutionary history of different photoreceptor lineages and their transduction mechanisms; moreover, isolated amphioxus photoreceptors offer unique advantages, because they are unambiguously identifiable and amenable to single-cell physiological assays. In the present study whole-cell patch clamp recording, pharmacological manipulations, and immunodetection were utilized to investigate light transduction in amphioxus photoreceptors. A G(q was identified and selectively localized to the photosensitive microvillar membrane, while the pivotal role of phospholipase C was established pharmacologically. The photocurrent was profoundly depressed by IP₃ receptor antagonists, highlighting the importance of IP₃ receptors in light signaling. By contrast, surrogates of diacylglycerol (DAG, as well as poly-unsaturated fatty acids failed to activate a membrane conductance or to alter the light response. The results strengthen the notion that calcium released from the ER via IP₃-sensitive channels may fulfill a key role in conveying--directly or indirectly--the melanopsin-initiated light signal to the photoconductance; moreover, they challenge the dogma that microvillar photoreceptors and phoshoinositide-based light transduction are a prerogative of invertebrate eyes.

  1. Development of oral and branchial muscles in lancelet larvae of Branchiostoma japonicum.

    Science.gov (United States)

    Yasui, Kinya; Kaji, Takao; Morov, Arseniy R; Yonemura, Shigenobu

    2014-04-01

    The perforated pharynx has generally been regarded as a shared characteristic of chordates. However, there still remains phylogenetic ambiguity between the cilia-driven system in invertebrate chordates and the muscle-driven system in vertebrates. Giant larvae of the genus Asymmetron were reported to develop an orobranchial musculature similar to that of vertebrates more than 100 years ago. This discovery might represent an evolutionary link for the chordate branchial system, but few investigations of the lancelet orobranchial musculature have been completed since. We studied staged larvae of a Japanese population of Branchiostoma japonicum to characterize the developmental property of the orobranchial musculature. The larval mouth and the unpaired primary gills develop well-organized muscles. These muscles function only as obturators of the openings without antagonistic system. As the larval mouth enlarged posteriorly to the level of the ninth myomere, the oral musculature was fortified accordingly without segmental patterning. In contrast, the iterated branchial muscles coincided with the dorsal myomeric pattern before metamorphosis, but the pharynx was remodeled dynamically irrespective of the myomeric pattern during metamorphosis. The orobranchial musculature disappeared completely during metamorphosis, and adult muscles in the oral hood and velum, as well as on the pterygial coeloms developed independently. The lancelet orobranchial musculature is apparently a larval adaptation to prevent harmful intake. However, vestigial muscles appeared transiently with the secondary gill formation suggest a bilateral ancestral state of muscular gills, and a segmental pattern of developing branchial muscles without neural crest and placodal contributions is suggestive of a precursor of vertebrate branchiomeric pattern.

  2. An amphioxus winged helix/forkhead gene, AmphiFoxD: insights into vertebrate neural crest evolution

    Science.gov (United States)

    Yu, Jr-Kai; Holland, Nicholas D.; Holland, Linda Z.

    2002-01-01

    During amphioxus development, the neural plate is bordered by cells expressing many genes with homologs involved in vertebrate neural crest induction. However, these amphioxus cells evidently lack additional genetic programs for the cell delaminations, migrations, and differentiations characterizing definitive vertebrate neural crest. We characterize an amphioxus winged helix/forkhead gene (AmphiFoxD) closely related to vertebrate FoxD genes. Phylogenetic analysis indicates that the AmphiFoxD is basal to vertebrate FoxD1, FoxD2, FoxD3, FoxD4, and FoxD5. One of these vertebrate genes (FoxD3) consistently marks neural crest during development. Early in amphioxus development, AmphiFoxD is expressed medially in the anterior neural plate as well as in axial (notochordal) and paraxial mesoderm; later, the gene is expressed in the somites, notochord, cerebral vesicle (diencephalon), and hindgut endoderm. However, there is never any expression in cells bordering the neural plate. We speculate that an AmphiFoxD homolog in the common ancestor of amphioxus and vertebrates was involved in histogenic processes in the mesoderm (evagination and delamination of the somites and notochord); then, in the early vertebrates, descendant paralogs of this gene began functioning in the presumptive neural crest bordering the neural plate to help make possible the delaminations and cell migrations that characterize definitive vertebrate neural crest. Copyright 2002 Wiley-Liss, Inc.

  3. Amphioxus and lamprey AP-2 genes: implications for neural crest evolution and migration patterns

    Science.gov (United States)

    Meulemans, Daniel; Bronner-Fraser, Marianne

    2002-01-01

    The neural crest is a uniquely vertebrate cell type present in the most basal vertebrates, but not in cephalochordates. We have studied differences in regulation of the neural crest marker AP-2 across two evolutionary transitions: invertebrate to vertebrate, and agnathan to gnathostome. Isolation and comparison of amphioxus, lamprey and axolotl AP-2 reveals its extensive expansion in the vertebrate dorsal neural tube and pharyngeal arches, implying co-option of AP-2 genes by neural crest cells early in vertebrate evolution. Expression in non-neural ectoderm is a conserved feature in amphioxus and vertebrates, suggesting an ancient role for AP-2 genes in this tissue. There is also common expression in subsets of ventrolateral neurons in the anterior neural tube, consistent with a primitive role in brain development. Comparison of AP-2 expression in axolotl and lamprey suggests an elaboration of cranial neural crest patterning in gnathostomes. However, migration of AP-2-expressing neural crest cells medial to the pharyngeal arch mesoderm appears to be a primitive feature retained in all vertebrates. Because AP-2 has essential roles in cranial neural crest differentiation and proliferation, the co-option of AP-2 by neural crest cells in the vertebrate lineage was a potentially crucial event in vertebrate evolution.

  4. Characterization and developmental expression of AmphiMef2 gene in amphioxus

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Myocyte enhancer factor 2 proteins are members of MADS family of transcription factors, which can control the expression of muscle-specific genes in vertebrates. However, not all Mef2 genes are essential for muscle development in invertebrates. Here we have isolated a full-length cDNA from amphioxus, designated AmphiMef2. The predicted amino acid sequence has highly conserved MADS and MEF2 domains, showing higher identity with the corresponding regions of its homologues in vertebrates than those in invertebrates. Results from whole-mount in situ hybridization show that the expression of AmphiMef2 initially appears in the presomitic mesoderm at early neurula stage, then the transcripts are detected in both the somites and the unsegmented presomitic mesoderm. At 36 h larval stage, the expression is only detected in the posterior somites. By 48 h larval stage, the expression is shifted to the preoral pit (a homologous organ to the vertebrate adenohypophysis) and persists until at least 72 h larval stage. The results suggest that AmphiMef2 may be not only involved in the myogenesis but also the development or function of the preoral pit in amphioxus.

  5. Differential mesodermal expression of two amphioxus MyoD family members (AmphiMRF1 and AmphiMRF2)

    Science.gov (United States)

    Schubert, Michael; Meulemans, Daniel; Bronner-Fraser, Marianne; Holland, Linda Z.; Holland, Nicholas D.

    2003-01-01

    To explore the evolution of myogenic regulatory factors in chordates, we isolated two MyoD family genes (AmphiMRF1 and AmphiMRF2) from amphioxus. AmphiMRF1 is first expressed at the late gastrula in the paraxial mesoderm. As the first somites form, expression is restricted to their myotomal region. In the early larva, expression is strongest in the most anterior and most posterior somites. AmphiMRF2 transcription begins at mid/late gastrula in the paraxial mesoderm, but never spreads into its most anterior region. Through much of the neurula stage, AmphiMRF2 expression is strong in the myotomal region of all somites except the most anterior pair; by late neurula expression is downregulated except in the most posterior somites forming just rostral to the tail bud. These two MRF genes of amphioxus have partly overlapping patterns of mesodermal expression and evidently duplicated independent of the diversification of the vertebrate MRF family.

  6. Evolution of the reproductive endocrine system in chordates.

    Science.gov (United States)

    Kubokawa, Kaoru; Tando, Yukiko; Roy, Sonali

    2010-07-01

    The cephalochordate, amphioxus, is phylogenetically placed at the most primitive position in the chordate clade. Despite many studies on the endocrine system of amphioxus, definitive evidence has not been reported for the presence an endocrine system comparable to the pituitary-gonadal axis, which is important in the regulation of reproduction in vertebrates. Recent genome analyses in the amphioxus, Branchiostoma floridae, showed that it does not have any pituitary hormone genes except the thyrostimulin gene. Thyrostimulin is a heterodimeric glycoprotein hormone consisting of α and β subunits, and is present in various organs of vertebrates. Analyses of a phylogenetic tree and a synteny suggest that amphioxus' thyrostimulin is an ancestral type of the glycoprotein hormones in chordates. In addition, genes for sex steroidogenic enzymes belonging to the CYP family were found in the genome sequences. The conversion pathway of sex steroids from cholesterol to estrogen, androgen, and major sex steroids was also identified in the gonads of amphioxus in vitro. Furthermore, we demonstrated the expression of genes encoding thyrostimulin and sex steroidogenic enzymes by an in situ hybridization technique. Here, we discuss the evolution of hormones and reproductive functions in the neuroendocrine control system of chordates.

  7. The identification of lymphocyte-like cells and lymphoid-related genes in amphioxus indicates the twilight for the emergence of adaptive immune system.

    Directory of Open Access Journals (Sweden)

    Gonghua Huang

    Full Text Available To seek evidence of a primitive adaptive immune system (AIS before vertebrate, we examined whether lymphocytes or lymphocyte-like cells and the related molecules participating in the lymphocyte function existed in amphioxus. Anatomical analysis by electron microscopy revealed the presence of lymphocyte-like cells in gills, and these cells underwent morphological changes in response to microbial pathogens that are reminiscent of those of mammalian lymphocytes executing immune response to microbial challenge. In addition, a systematic comparative analysis of our cDNA database of amphioxus identified a large number of genes whose vertebrate counterparts are involved in lymphocyte function. Among these genes, several genes were found to be expressed in the vicinity of the lymphocyte-like cells by in situ hybridization and up-regulated after exposure to microbial pathogens. Our findings in the amphioxus indicate the twilight for the emergence of AIS before the invertebrate-vertebrate transition during evolution.

  8. Localization of aromatase in the nervous system, Hatschek's pit and gonad of amphioxus by in situ hybridization and immunocytochemistry%芳香化酶在文昌鱼神经系统、哈氏窝和性腺特异性定位:原位杂交和免疫细胞化学研究

    Institute of Scientific and Technical Information of China (English)

    方永强; 翁幼竹; 黄威权; 孙岚

    2003-01-01

    芳香化酶活性发现在脊椎动物脑、脑垂体和性腺中,但在文昌鱼脑和哈氏窝的组织特异性定位尚无可利用资料.本文用免疫细胞化学和原位杂交技术,首次发现芳香化酶活性组织特异性定位在幼年和性腺发育不同时期雌、雄文昌鱼神经系统(脑和脊髓)、轮器、哈氏窝和性腺中.芳香化酶蛋白和转录物在前脑、中脑、脊髓、轮器和哈氏窝十分丰富,而后脑、早期卵巢和精巢不够丰富;没有芳香化酶表达的部位是哈氏窝另两种细胞(不规则形细胞和带纤毛粘液细胞)以及成熟卵巢和精巢;芳香化酶免疫活性物质分布在胞质,核为阴性.芳香化酶在文昌鱼神经系统、哈氏窝和性腺的分布模式与低等脊椎动物中的分布模式极为类似,尤其是芳香化酶在脑内调节哈氏窝分泌活动的神经内分泌中枢表达,并形成类似脊椎动物的文昌鱼原始的脑-芳香化酶调节系统.这些结果有力地证明,文昌鱼脑和哈氏窝高水平的芳香化酶活性像在其它脊椎动物中一样,对局部介导睾酮芳香化起着关键作用,同时还可能影响脑-芳香化酶系统参与调节哈氏窝的分泌活动[动物学报49(6):800~806,2003].%Aromatase activity has been found in the brain and pituitary as well as gonads of vertebrates, but no information is available on the specific localization in the brain and Hatschek's pit (primitive pituitary) of amphioxus (Branchios-toma belcheri). Using immunocytochemistry and in situ hybridization, we studied definitive tissue specific localization of aromatase in the nervous system (brain and spinal cord), wheel organ, Hatschek's pit and gonads at young and adult female and male amphioxus at different stages of gonadal development. Aromatase protein and its mRNA were abundant in the forebrain, midbrain, spinal cord, wheel organ and Hatschek's pit, but not abundant in the hindbrain or early ovary and testis. No aromatase expression

  9. A single three-dimensional chromatin compartment in amphioxus indicates a stepwise evolution of vertebrate Hox bimodal regulation.

    Science.gov (United States)

    Acemel, Rafael D; Tena, Juan J; Irastorza-Azcarate, Ibai; Marlétaz, Ferdinand; Gómez-Marín, Carlos; de la Calle-Mustienes, Elisa; Bertrand, Stéphanie; Diaz, Sergio G; Aldea, Daniel; Aury, Jean-Marc; Mangenot, Sophie; Holland, Peter W H; Devos, Damien P; Maeso, Ignacio; Escrivá, Hector; Gómez-Skarmeta, José Luis

    2016-03-01

    The HoxA and HoxD gene clusters of jawed vertebrates are organized into bipartite three-dimensional chromatin structures that separate long-range regulatory inputs coming from the anterior and posterior Hox-neighboring regions. This architecture is instrumental in allowing vertebrate Hox genes to pattern disparate parts of the body, including limbs. Almost nothing is known about how these three-dimensional topologies originated. Here we perform extensive 4C-seq profiling of the Hox cluster in embryos of amphioxus, an invertebrate chordate. We find that, in contrast to the architecture in vertebrates, the amphioxus Hox cluster is organized into a single chromatin interaction domain that includes long-range contacts mostly from the anterior side, bringing distant cis-regulatory elements into contact with Hox genes. We infer that the vertebrate Hox bipartite regulatory system is an evolutionary novelty generated by combining ancient long-range regulatory contacts from DNA in the anterior Hox neighborhood with new regulatory inputs from the posterior side.

  10. Roles of polysaccharide from Branchiostoma belcheri in anti-DNA oxidation and anti-tumor activity in S180 mice

    Science.gov (United States)

    Liang, Hui; Zhang, Shicui

    2009-11-01

    In this study, we isolated a polysaccharide from Branchiostoma belcheri (PBB) by enzymatic protein hydrolysis and alcohol precipitation. We investigated the effects of PBB supplementation on DNA oxidation and growth of the transplanted tumor cells Sarcoma (S180) in mice. Sixty healthy Kunming mice weighing between 18 and 25 g were randomly assigned to 6 groups, each consisting of 10 animals. All the mice, except for the blank control group, were inoculated with S180 sarcoma cells into the axilla of the left foreleg. PBB was given to mice by gavage at doses of 0 (model control), 25, 50, or 100 mg/kg b.w. in 0.2 ml saline for 30 days. The fifth group of S180-mice was given cytoxan (50 mg/kg) by peritoneal injection as a positive control group. The animals had free access to food and water. The mice were sacrificed after the final treatment and blood was quickly collected. Spontaneous and oxidized DNA damage of peripheral lymphocytes induced by H2O2 were analyzed by SCGE. O6-methyl-guanine (O6-MeG) was measured by high-performance capillary zone electrophoresis. The average tumor weights (0.856-1.118 g) of the three PBB groups were significantly lower than that of the model control group (1.836 g) ( p<0.05). The tumor inhibition ratios of the PBB groups were 39.1%-53.4% and similar to the cytoxan positive group (57.5%). There were no significant differences in spontaneous DNA damage in peripheral lymphocytes among the groups. The oxidative DNA damage induced by 10 µmol/L H2O2 in the 50 and 100 mg/kg b.w. groups were 246.1 AU and 221.7 AU, respectively, both of which were significantly lower than that in the model group (289.0 AU; p<0.05). The plasma concentrations of O6-MeG in the 25, 50, and 100 mg/kg supplemented groups were 2.09 µmol/L, 1.86 µmol/L, and 1.63 µmol/L, respectively, all of which were significantly lower than that of the model group (2.67 µmol/L; p<0.05). These results indicated that PBB may have antioxidative activity and thus reduce oxidation

  11. Late Gale in Florida

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A severe gale in August of 1856 caused a lot of destruction in Florida. Ships and warehouses were damaged, a lighthouse was destroyed, crops were ruined, and several...

  12. Florida Abandoned Vessel Inventory

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — NOAA Abandoned Vessel Project Data for Florida. Abandoned vessels pose a significant threat to the NOAA Trust resources through physical destruction of coral...

  13. AmphiFoxQ2, a novel winged helix/forkhead gene, exclusively marks the anterior end of the amphioxus embryo

    Science.gov (United States)

    Yu, Jr-Kai; Holland, Nicholas D.; Holland, Linda Z.

    2003-01-01

    A full-length FoxQ-related gene (AmphiFoxQ2) was isolated from amphioxus. Expression is first detectable in the animal/anterior hemisphere at the mid blastula stage. The midpoint of this expression domain coincides with the anterior pole of the embryo and is offset dorsally by about 20 degrees from the animal pole. During the gastrula stage, expression is limited to the anterior ectoderm. By the early neurula stage, expression remains in the anterior ectoderm and also appears in the adjacent anterior mesendoderm. By the early larval stages, expression is detectable in the anteriormost ectoderm and in the rostral tip of the notochord. AmphiFoxQ2 is never expressed anywhere except at the anterior tip of amphioxus embryos and larvae. This is the first gene known that exclusively marks the anterior pole of chordate embryos. It may, therefore, play an important role in establishing and/or maintaining the anterior/posterior axis.

  14. Florida Hydrogen Initiative

    Energy Technology Data Exchange (ETDEWEB)

    Block, David L

    2013-06-30

    The Florida Hydrogen Initiative (FHI) was a research, development and demonstration hydrogen and fuel cell program. The FHI program objectives were to develop Florida?s hydrogen and fuel cell infrastructure and to assist DOE in its hydrogen and fuel cell activities The FHI program funded 12 RD&D projects as follows: Hydrogen Refueling Infrastructure and Rental Car Strategies -- L. Lines, Rollins College This project analyzes strategies for Florida's early stage adaptation of hydrogen-powered public transportation. In particular, the report investigates urban and statewide network of refueling stations and the feasibility of establishing a hydrogen rental-car fleet based in Orlando. Methanol Fuel Cell Vehicle Charging Station at Florida Atlantic University ? M. Fuchs, EnerFuel, Inc. The project objectives were to design, and demonstrate a 10 kWnet proton exchange membrane fuel cell stationary power plant operating on methanol, to achieve an electrical energy efficiency of 32% and to demonstrate transient response time of less than 3 milliseconds. Assessment of Public Understanding of the Hydrogen Economy Through Science Center Exhibits, J. Newman, Orlando Science Center The project objective was to design and build an interactive Science Center exhibit called: ?H2Now: the Great Hydrogen Xchange?. On-site Reformation of Diesel Fuel for Hydrogen Fueling Station Applications ? A. Raissi, Florida Solar Energy Center This project developed an on-demand forecourt hydrogen production technology by catalytically converting high-sulfur hydrocarbon fuels to an essentially sulfur-free gas. The removal of sulfur from reformate is critical since most catalysts used for the steam reformation have limited sulfur tolerance. Chemochromic Hydrogen Leak Detectors for Safety Monitoring ? N. Mohajeri and N. Muradov, Florida Solar Energy Center This project developed and demonstrated a cost-effective and highly selective chemochromic (visual) hydrogen leak detector for safety

  15. Structure of the red fluorescent protein from a lancelet (Branchiostoma lanceolatum): a novel GYG chromophore covalently bound to a nearby tyrosine

    Energy Technology Data Exchange (ETDEWEB)

    Pletnev, Vladimir Z., E-mail: vzpletnev@gmail.com; Pletneva, Nadya V.; Lukyanov, Konstantin A.; Souslova, Ekaterina A.; Fradkov, Arkady F.; Chudakov, Dmitry M.; Chepurnykh, Tatyana; Yampolsky, Ilia V. [Russian Academy of Sciences, Moscow (Russian Federation); Wlodawer, Alexander [National Cancer Institute, Frederick, MD 21702 (United States); Dauter, Zbigniew [National Cancer Institute, Argonne, IL 60439 (United States); Pletnev, Sergei, E-mail: vzpletnev@gmail.com [National Cancer Institute, Argonne, IL 60439 (United States); SAIC-Frederick, Argonne, IL 60439 (United States); Russian Academy of Sciences, Moscow (Russian Federation)

    2013-09-01

    The crystal structure of the novel red emitting fluorescent protein from lancelet Branchiostoma lanceolatum (Chordata) revealed an unusual five residues cyclic unit comprising Gly58-Tyr59-Gly60 chromophore, the following Phe61 and Tyr62 covalently bound to chromophore Tyr59. A key property of proteins of the green fluorescent protein (GFP) family is their ability to form a chromophore group by post-translational modifications of internal amino acids, e.g. Ser65-Tyr66-Gly67 in GFP from the jellyfish Aequorea victoria (Cnidaria). Numerous structural studies have demonstrated that the green GFP-like chromophore represents the ‘core’ structure, which can be extended in red-shifted proteins owing to modifications of the protein backbone at the first chromophore-forming position. Here, the three-dimensional structures of green laGFP (λ{sub ex}/λ{sub em} = 502/511 nm) and red laRFP (λ{sub ex}/λ{sub em} ≃ 521/592 nm), which are fluorescent proteins (FPs) from the lancelet Branchiostoma lanceolatum (Chordata), were determined together with the structure of a red variant laRFP-ΔS83 (deletion of Ser83) with improved folding. Lancelet FPs are evolutionarily distant and share only ∼20% sequence identity with cnidarian FPs, which have been extensively characterized and widely used as genetically encoded probes. The structure of red-emitting laRFP revealed three exceptional features that have not been observed in wild-type fluorescent proteins from Cnidaria reported to date: (i) an unusual chromophore-forming sequence Gly58-Tyr59-Gly60, (ii) the presence of Gln211 at the position of the conserved catalytic Glu (Glu222 in Aequorea GFP), which proved to be crucial for chromophore formation, and (iii) the absence of modifications typical of known red chromophores and the presence of an extremely unusual covalent bond between the Tyr59 C{sup β} atom and the hydroxyl of the proximal Tyr62. The impact of this covalent bond on the red emission and the large Stokes shift (

  16. The Maya of Florida.

    Science.gov (United States)

    Burns, Allan F.

    1989-01-01

    Discusses the Maya people who fled Guatemala due to a civil war and illegally entered the U.S. and settled in Florida. Presents a picture of their living conditions, employment opportunities, cultural traditions, community development, and family organization. Discusses a Kanjobal Association and the CORN-MAYA program, and explains immigration…

  17. Conservation: saving Florida's manatees

    Science.gov (United States)

    Bonde, Robert K.

    2008-01-01

    Robert K. Bonde of the U.S. Geological Survey writes about the protected population of manatees in Crystal River, Florida, including information about the threats they face as they migrate in and out of protected waters. Photographer Carol Grant shares images of "Angel," a newborn manatee she photographed early one winter morning.

  18. Adapting Bulls to Florida

    Science.gov (United States)

    The adaptation of bulls used for natural breeding purposes to the Gulf Coast region of the United States including all of Florida is an important topic. Nearly 40% of the U.S. cow/calf population resides in the Gulf Coast and Southeast. Thus, as A.I. is relatively rare, the number of bulls used for ...

  19. Migrant Programs in Florida.

    Science.gov (United States)

    National Migrant Information Clearinghouse, Austin, TX. Juarez-Lincoln Center.

    As the last of 3 directories, this lists services available to migrants in Florida. Migrant programs, Community Action Agencies, and labor camps in the state are identified by county. Information for each county includes total population, estimated migrant population, migrant labor demand, estimated migrant wages, crops, work periods, migrant…

  20. Florida Hydrogen Initiative

    Energy Technology Data Exchange (ETDEWEB)

    Block, David L

    2013-06-30

    The Florida Hydrogen Initiative (FHI) was a research, development and demonstration hydrogen and fuel cell program. The FHI program objectives were to develop Florida?s hydrogen and fuel cell infrastructure and to assist DOE in its hydrogen and fuel cell activities The FHI program funded 12 RD&D projects as follows: Hydrogen Refueling Infrastructure and Rental Car Strategies -- L. Lines, Rollins College This project analyzes strategies for Florida's early stage adaptation of hydrogen-powered public transportation. In particular, the report investigates urban and statewide network of refueling stations and the feasibility of establishing a hydrogen rental-car fleet based in Orlando. Methanol Fuel Cell Vehicle Charging Station at Florida Atlantic University ? M. Fuchs, EnerFuel, Inc. The project objectives were to design, and demonstrate a 10 kWnet proton exchange membrane fuel cell stationary power plant operating on methanol, to achieve an electrical energy efficiency of 32% and to demonstrate transient response time of less than 3 milliseconds. Assessment of Public Understanding of the Hydrogen Economy Through Science Center Exhibits, J. Newman, Orlando Science Center The project objective was to design and build an interactive Science Center exhibit called: ?H2Now: the Great Hydrogen Xchange?. On-site Reformation of Diesel Fuel for Hydrogen Fueling Station Applications ? A. Raissi, Florida Solar Energy Center This project developed an on-demand forecourt hydrogen production technology by catalytically converting high-sulfur hydrocarbon fuels to an essentially sulfur-free gas. The removal of sulfur from reformate is critical since most catalysts used for the steam reformation have limited sulfur tolerance. Chemochromic Hydrogen Leak Detectors for Safety Monitoring ? N. Mohajeri and N. Muradov, Florida Solar Energy Center This project developed and demonstrated a cost-effective and highly selective chemochromic (visual) hydrogen leak detector for safety

  1. Libraries in Florida: MedlinePlus

    Science.gov (United States)

    ... Library → Libraries in Florida URL of this page: https://medlineplus.gov/libraries/florida.html Libraries in Florida ... Pancake Atlantis, FL 33462-1197 561-548-3480 http://opac.libraryworld.com/cgi-bin/opac.pl?command= ...

  2. Orlando, Florida, USA

    Science.gov (United States)

    1991-01-01

    Much of central Florida, including this detailed view of Orlando (28.5N, 81.0W) can be seen in this single photo. Disney World is at the top center of the scene and the crescent shaped Lake Tohopekaliga is near the bottom. The large round lakes are believed to be sinkholes formed during glacial times when ocean levels were several hundred feet lower than the present. Linear patterns east of Orlando are thought to be ancient shoreline ridges.

  3. The Florida Library History Project.

    Science.gov (United States)

    Jasper, Catherine; McCook, Kathleen de la Pena

    The Florida Library History Project (FLHP) began in January 1998. Letters requesting histories were sent to all public libraries in Florida with follow-up letters sent after an initial response was received from the libraries. E-mail messages were sent out to FL-LIB listservs encouraging participation in the project. A poster session was presented…

  4. Characterization of bbtTICAM from amphioxus suggests the emergence of a MyD88-independent pathway in basal chordates

    Institute of Scientific and Technical Information of China (English)

    ManyiYang; ShaochunYuan; Shengfeng Huang; Jun Li; Liqun Xu; Huiqing Huang; Xin Tao; Jian peng; Anlong Xu

    2011-01-01

    The MyD88-independent pathway,one of the two crucial TLR signaling routes,Is thought to be a vertebrate innovation.However,a novel Toll/interleukin-1 receptor (TIR) adaptor,designated bbtTICAM,which was identified in the basal chordate amphioxus,links this pathway to invertebrates.The protein architecture of bbtTICAM is similar to that of vertebrate TICAM1 (TIR-containing adaptor molecule-1,also known as TRIF),while phylogenetic analysis based on the TIR domain indicated that bbtTICAM is the oldest ortholog of vertebrate TICAMI and TICAM2(TIR-containing adaptor molecule-2,also known as TRAM).Similar to human TICAM1,bbtTICAM activates NF-κB in a MyD88-independent manner by interacting with receptor interacting protein (RIP) via its RHIM motif.Such activation requires bbtTICAM to form homodimers in endosomes,and it may be negatively regulated by amphioxus SARM (sterile a and armadillo motif-containing protein) and TRAF2.However,bbtTICAM did not induce the production of type I interferon.Thus,our study not only presents the ancestral features of vertebrate TICAM I and TICAM2,but also reveals the evolutionary origin of the MyD88-independent pathway from basal chordates,which will aid in understanding the development of the vertebrate TLR network.

  5. Dengue in Florida (USA

    Directory of Open Access Journals (Sweden)

    Jorge R. Rey

    2014-12-01

    Full Text Available Florida (USA, particularly the southern portion of the State, is in a precarious situation concerning arboviral diseases. The geographic location, climate, lifestyle, and the volume of travel and commerce are all conducive to arbovirus transmission. During the last decades, imported dengue cases have been regularly recorded in Florida, and the recent re-emergence of dengue as a major public health concern in the Americas has been accompanied by a steady increase in the number of imported cases. In 2009, there were 28 cases of locally transmitted dengue in Key West, and in 2010, 65 cases were reported. Local transmission was also reported in Martin County in 2013 (29 cases, and isolated locally transmitted cases were also reported from other counties in the last five years. Dengue control and prevention in the future will require close cooperation between mosquito control and public health agencies, citizens, community and government agencies, and medical professionals to reduce populations of the vectors and to condition citizens and visitors to take personal protection measures that minimize bites by infected mosquitoes.

  6. Epidemiology of Ciguatera in Florida

    OpenAIRE

    Radke, Elizabeth G.; Reich, Andrew; Morris, John Glenn

    2015-01-01

    Ciguatera is the most commonly reported marine food-borne illness worldwide. Because there is a biological plausibility that ciguatera may be impacted by long-term climate variability and Florida is on the northern border of the geographic distribution of ciguatera, it is important to update our understanding of its epidemiology in Florida. We performed an analysis of 291 reports in Florida from 2000 to 2011 and an e-mail survey of 5,352 recreational fishers to estimate incidence and underrep...

  7. 文昌鱼SMYD家族基因的系统进化分析%Phylogenetic Analysis of the Amphioxus SMYD Gene Family

    Institute of Scientific and Technical Information of China (English)

    郭泉阳; 李红岩

    2015-01-01

    SMYD( SET and MYND domain containing,SMYD) are a class of proteins containing the SET func-tional domains. They play an important role in the regulation of chromosomal gene expression,cell cycle con-trol, cell division, differentiation and development. It is known that drosophila and vertebrates contain Smyd genes and scholars have done much research on the expression, function and evolution of these genes. Howev-er, research on the chordate SMYD family genes is limited. This article searched the cephalochordate amphioxus genome and found that amphioxus had six possible Smyd genes. Analysis of the functional domains of these Smyd genes showed that:amphioxus-related genes and those of other studied species,such as drosophi-la,xenopus,mouse,human are alike,all in different chromosomes. Analysis of gene structures shows that 3 Smyd genes of the amphioxus,2 of zebrafish and 4 of human have a SCOP functional domain in addition to the SET functional domains. Analysis of gene structure show that the genetic structure of vertebrates is conserva-tive,but invertebrates and chordata are less conservative. The results of phylogenetic analysis show that only two of the six amphioxus Smyd genes are high homologous with other species and the other four are relatively low. This paper aims to conduct a preliminary phylogenetic analysis of amphioxus SMYD family. What roles the Smyd genes in chordate amphioxus perform,and how they realize their functions still need further studies.%SMYD( SET and MYND domain containing,SMYD)是一类含有SET功能域的蛋白,在染色体的调节、基因的表达、细胞生长周期的控制、细胞的分裂、分化及发育等方面具有重要作用。目前已知在果蝇、脊椎动物中均含有Smyd基因,且这些基因的进化、表达及功能均有很多研究,但关于脊索动物SMYD家族基因的研究则很少。本文对头索动物文昌鱼的基因组进行搜索,发现文昌鱼有6个可能的Smyd基因。对Smyd基因在

  8. 78 FR 43197 - Duke Energy Florida, Inc.; Florida Power & Light Company; Tampa Electric Company; Orlando...

    Science.gov (United States)

    2013-07-19

    ... Company; Orlando Utilities Commission; Notice of Compliance Filings Take notice that on July 10, 2013, Duke Energy Florida, Inc., Florida Power & Light Company, Tampa Electric Company, and Orlando...

  9. Florida Panther Reintroduction Feasibility Study

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Summary and final report of a Florida Fish and Wildlife Conservation Commission study to evalaute initial stocking of mountain lion populations in northern...

  10. 2006 Volusia County, Florida Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is the lidar data for Volusia County, Florida, approximately 1,432 square miles, acquired in early March of 2006. A total of 143 flight lines of Lidar...

  11. Backtalk: Adult Services in Florida.

    Science.gov (United States)

    Giblon, Della L.

    1979-01-01

    Initiates a new state emphasis for the column by highlighting recent public library programs and services for adults in Florida. Music, photography, and women's programs offered by the Leon County Public Library are described in more detail. (JD)

  12. Spaceport Florida Authority: Business Plan

    Science.gov (United States)

    1996-01-01

    The Spaceport Florida Authority (SFA) was established under Florida Statute by the Governor and Legislature to assist the development of our nation's space transportation industry and to generate new space-related jobs, investment and opportunities statewide. Included in the Authorities' business plan is the statement of work and list of team members involved in creating the report, SFA's current operating concept, market analysis, assessment of accomplishments, a sample operating concept and a "roadmap to success".

  13. Epidemiology of Ciguatera in Florida.

    Science.gov (United States)

    Radke, Elizabeth G; Reich, Andrew; Morris, John Glenn

    2015-08-01

    Ciguatera is the most commonly reported marine food-borne illness worldwide. Because there is a biological plausibility that ciguatera may be impacted by long-term climate variability and Florida is on the northern border of the geographic distribution of ciguatera, it is important to update our understanding of its epidemiology in Florida. We performed an analysis of 291 reports in Florida from 2000 to 2011 and an e-mail survey of 5,352 recreational fishers to estimate incidence and underreporting and identify high risk demographic groups, fish types, and catch locations. Incidence was 5.6 per 100,000 adjusted for underreporting. Hispanics had the highest incidence rate (relative risk [RR] = 3.4) and were more likely to eat barracuda than non-Hispanics. The most common catch locations for ciguatera-causing fish were the Bahamas and Florida Keys. Cases caused by fish from northern Florida were infrequent. These results indicate that ciguatera incidence is higher than estimated from public health reports alone. There is little evidence that incidence or geographic range has increased because of increased seawater temperatures since earlier studies.

  14. CLONING OF QINGDAO AMPHIOXUS HEDGEHOG GENE,A MOLECULE INVOLVED IN INTERCELLULAR SIGNALING OF DEVELOPMENT%青岛文昌鱼发育信号传导相关基因hedgehog的克隆

    Institute of Scientific and Technical Information of China (English)

    毛炳宇; 张燕君; 张红卫

    2001-01-01

    hedgehog家族基因在动物胚胎多种发育过程的信号传导中起着关键作用.采用简并引物、套式PCR和RT-PCR方法获得青岛文昌鱼hedgehog基因片段,并对其所推测的氨基酸序列与hh基因家族成员小鼠Shh、Ihh、Dhh,鸡、爪蟾和斑马鱼Shh及果蝇hh等的相应片段进行同源性分析.它们的同源性分别为56%,53%,50%,53%,53%,53%和45%.研究结果支持文昌鱼具有1个,可能也仅有1个hedgehog家族基因.%The degenerate primers were designed according to the conserved sequences of hedgehog gene family. A single hh gene fragment of Qindao amphioxus was cloned by PCR and RT-PCR. The homology analysis of deduced amino acid sequence showed that Qingdao amphioxus hh had 56%, 53%, 50%, 53%, 53%, 53% and 45% similarities with mouse Shh, Ihh, Dhh, chick, Xenopus, zebrafish Shh, and Drosophila hh respectively. The results showed that amphioxus contained one and probably only one hh gene.

  15. Hydrology of Southeast Florida and Associated Topics.

    Science.gov (United States)

    Monsour, William, Comp.; Moyer, Maureen, Comp.

    This booklet deals with the hydrology of southeastern Florida. It is designed to provide the citizen, teacher, or student with hydrological information, to promote an understanding of water resources, and to initiate conservation practices within Florida communities. The collection of articles within the booklet deal with Florida water resources…

  16. 文昌鱼胚胎整装荧光原位杂交技术的建立%Whole - mounted fluorescent in situ hybridization of amphioxus embryos

    Institute of Scientific and Technical Information of China (English)

    喻丹; 张培军

    2005-01-01

    文昌鱼(Branchiostoma belcheri tsingtauense)是进化发育生物学研究的重要模式生物,文昌鱼胚胎整装荧光原位杂交(WFISH,whole-mounted fluorescent in situ hybridization)技术将有助于鉴定文昌鱼胚胎发育过程中具有重要调控作用的功能基因.报告了文昌鱼胚胎整装荧光原位杂交技术,用以快速灵敏分析特定基因在文昌鱼胚胎发育过程中的时空表达图式.用体外转录合成的地高辛标记文昌鱼FGFR基因的反义RNA探针,检测到FGFR在文昌鱼原肠胚中表达于发生内陷的中内胚层细胞中,而预定发育为外胚层的细胞不表达FGFR.

  17. 2008 NWFWMD (Northwest Florida Water Management District) Florida LiDAR: Inland Okaloosa County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a survey of inland Okaloosa County, Florida not covered in the 2008 Florida Department of Emergency...

  18. Woody biomass production systems for Florida

    Energy Technology Data Exchange (ETDEWEB)

    Rockwood, D.L.; Pathak, N.N.; Satapathy, P.C. (Florida Univ., Gainesville, FL (United States). Dept. of Forestry)

    1993-01-01

    Woody biomass production research in Florida has addressed genetic improvement, coppice productivity, clonal propagation, biomass properties, and economics of Eucalyptus and other species in short rotation, intensive culture systems. Improved E. grandis seedlings could more than double productivity, but exceptional clones offer more immediate potential in southern Florida. E. tereticornis and E. camaldulensis appear to have frost-resistance and good growth in central and southern Florida. For northern Florida, E. amplifolia has good frost-resilience and coppicing ability. Eucalytpus species are suitable for fermentation processes. Other promising species include Casuarina glauca and Taxodium distichum in southern Florida, and Sapium sebiferum state-wide. Break-even costs for biomass production systems with Eucalyptus range from approximately $2.00 to $4.00 GJ[sup -1]; short rotation culture appears feasible for slash pine in northern and central Florida but cannot yet be advised for sand pine. (author)

  19. Benthic Habitats of Estero Bay Area, Florida 1999 Substrate

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data produced for the Florida Fish and Wildlife Conservation Commission's Florida Marine Research Institute (FMRI) in partnership with the South Florida Water...

  20. Benthic Habitats of Estero Bay Area, Florida 1999 Geodatabase

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data produced for the Florida Fish and Wildlife Conservation Commission's Florida Marine Research Institute (FMRI) in partnership with the South Florida Water...

  1. Benthic Habitats of Estero Bay Area, Florida 1999 Biotic

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data produced for the Florida Fish and Wildlife Conservation Commission's Florida Marine Research Institute (FMRI) in partnership with the South Florida Water...

  2. Benthic Habitats of Estero Bay Area, Florida 1999 Geoform

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Data produced for the Florida Fish and Wildlife Conservation Commission's Florida Marine Research Institute (FMRI) in partnership with the South Florida Water...

  3. The Impact of One Florida Initiative on Florida's Public Law Schools: A Critical Race Theory Analysis

    Science.gov (United States)

    Hilton, Adriel A.; Gasman, Marybeth; Wood, J. Luke

    2013-01-01

    The purpose of this study is to examine the impact of the One Florida Initiative (OFI) on racial diversity in Florida's public law schools and legal profession using the lens of Critical Race Theory (CRT). This study seeks to determine what, if any, impact this event has had on recruitment, admissions, and enrollment of Florida's public schools of…

  4. Generating amphioxus Hedgehog knockout mutants and phenotype analysis%文昌鱼Hedgehog基因敲除和突变体表型分析

    Institute of Scientific and Technical Information of China (English)

    王慧; 李光; 王义权

    2015-01-01

    文昌鱼隶属脊索动物门头索动物亚门,是无脊椎动物到脊椎动物的过渡类群,其躯体结构简单,是研究胚胎发育的理想材料。本文以文昌鱼为实验对象,利用TALEN敲除技术对Hedgehog(Hh)基因在胚胎发育中的功能进行了研究。在文昌鱼Hh基因翻译起始位点下游附近选取TALEN目标位点,根据此序列组装相应TALEN重组质粒,体外合成mRNA,向未受精卵注射mRNA后,经体外受精获得F0代胚胎。效率分析显示,靶向该基因的TALEN mRNA可导致F0代胚胎在相应基因组区域发生突变的比例为34%。对部分F0个体所产配子筛查发现,TALEN引起的突变可进入配子,将其中1尾突变类型为8 bp缺失的雄性个体与野生型雌性配对获得F1群体,对F1群体逐尾筛查,从中获得多尾携带8 bp缺失的杂合子;这些杂合子相互配对所产的F2代胚胎,其中约有1/4个体在幼体早期出现躯体前端和尾向下弯曲、脊索前端腹侧的中胚层组织发育不全,不能开口等;随着幼体生长发育,躯体前端和尾部进一步卷曲,口部仍未形成,左右各形成一个口前窝,内柱和鳃裂位于躯体腹侧,最终因无口摄食而死亡。基因型分析发现,上述畸形胚胎均为Hh纯合突变体,其与杂合子及野生型比例分布符合孟德尔遗传定律,表明这些发育畸型的特征与Hh基因功能缺失有关。%The amphioxus is a promising animal model for evolutionary-developmental studies due to its key posi-tion on the animal phylogenetic tree. In the present study, we reported a genetically modified amphioxus strain on the Hedgehog (Hh) gene locus using the TALEN method. The result showed that our TALEN pair injection could bring about 34% mutations in the amphioxus Hh coding region. Further analysis on the F0 gametic DNA revealed that the mutations had entered into gametes. So, we paired one F0 male carrying an 8 bp deletion with a wild

  5. Phage therapy for Florida corals?

    Science.gov (United States)

    Kellogg, Christina A.

    2007-01-01

    Coral disease is a major cause of reef decline in the Florida Keys. Bacterium has been defined as the most common pathogen (disease-causing organism). Although much is being done to catalog coral diseases, map their locations, determine the causes of disease, or measure the rates of coral demise, very little research has been directed toward actually preventing or eliminating the diseases affecting coral and coral reef decline.

  6. Telecommunications in Florida: Training Materials for Teachers.

    Science.gov (United States)

    Eason, Mike; And Others

    1994-01-01

    Describes the use of the Florida Information Resource Network (FIRN) in Florida public schools. Highlights include electronic mail exchanges; online conferences; remote research; classroom resources; training initiatives for teachers to learn about telecommunications; access to other systems and databases; and inservice, hands-on teacher training.…

  7. Lessons Learned from the Florida Teletraining Project.

    Science.gov (United States)

    Martin, Barbara L.; And Others

    The Florida Teletraining Project (FTP) was funded by the Department of Defense to test the feasibility of using a video teletraining network (VTT) (two-way audio/two-way compressed video) to present military instruction to reservists in Florida. The program was to be conducted by two-year community colleges in collaboration with armed forces…

  8. Florida Has Power-Library Schools

    Science.gov (United States)

    Reynolds-Mixon, Sharon

    2010-01-01

    In this article, the author talks about the Florida Power-Library School (FPLS) program. She describes the why, who, what and how of the Florida Power-Library School initiative, as well as the favorable results for schools. Schools successfully completing this process see relationships among staff and community members strengthened. Library media…

  9. Peninsular Florida future scenarios integrated project

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This is a integrated scenario project to the Florida state line that incorporates updated critical land and water identification project layers with a decision...

  10. 2006 Volusia County Florida LiDAR

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is the lidar data for Volusia County, Florida, approximately 1,432 square miles, acquired in early March of 2006. A total of 143 flight lines of Lidar...

  11. Contaminant concentrations in Florida raptor eggs

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Inviable eggs from the nests of Florida bald eagles and ospreys were collected opportunistically from 1987 through 1989. Egg contents were analyzed for...

  12. Benthic Habitats of the Florida Keys

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The benthic habitats of the Florida Keys were mapped from a series of 450 aerial photographs. Ecologists outlined the boundaries of specific habitat types by...

  13. Southwest Florida Shelf Ecosystems Analysis Study

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Southwest Florida Shelf Ecosystems Analysis Study produced grain size analyses in the historic 073 format for 299 sea floor samples collected from October 25,...

  14. 50 CFR 32.28 - Florida.

    Science.gov (United States)

    2010-10-01

    ... report card and place it in an entrance fee canister each day prior to exiting the refuge. 12. All youth... after the Florida State Central Management Zone General Gun (antlered deer and wild hog) season...

  15. Mammal Research: Exotic Ungulates in Florida

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — A review, of the exotic ungulate industry in Florida was made by mailing questionnaires to exotic ungulate permittees, phone interviews, interviews with exotic...

  16. Biscayne Bay Florida Bottlenose Dolphin Studies

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — These data sets include a compilation of small vessel based studies of bottlenose dolphins that reside within Biscayne Bay, Florida, adjacent estuaries and nearshore...

  17. 2004 St. Johns County, Florida Lidar

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is the bare earth lidar data for St. Johns County, Florida, acquired in early January and February of 2004. This data was collected to develop...

  18. Mercury contamination in Florida panthers [Draft

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — As a result of the death of an apparently healthy, radio-collared female Florida panther (Felis concolor qoryi) (FP#27) in Everglades National Park in July 1989, we...

  19. Permit Review - Florida Gas Transmission Company (FGT)

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Branch of Air Quality Permit Review for Florida Gas Transmission Company and their expansion of Compressor Station Number 10 in Wiggins, Mississippi. The facility is...

  20. Florida, Bahamas, Cuba and Gulf Stream, USA

    Science.gov (United States)

    1992-01-01

    This unique photo offers a view of the Florida peninsula, western Bahamas, north central Cuba and the deep blue waters of the Gulf Stream, that hugs the east coast of Florida (27.0N, 82.0W). In addition to being an excellent photograph for showing the geographical relationships between the variety of landforms in this scene, the typical effect of the land-sea breeze is very much in evidence as few clouds over water, cumulus build up over landmass.

  1. Geology and hydrogeology of the Florida Keys

    Science.gov (United States)

    Halley, Robert B.; Vacher, H. L.; Shinn,

    1997-01-01

    This chapter discusses the geology and hydrogeology of the Florida Keys, and focuses on the islands formed of Pleistocene limestone. These islands, which are crossed when driving from Miami to Key West, are typically regarded as "the Florida Keys." The outstanding and fragile character of ecosystems on and around the Florida Keys has prompted State and Federal efforts to protect and preserve the remaining public portions of the region. The Florida Keys were largely ignored during the sixteenth, seventeenth, and eighteenth centuries, although the waters just offshore provided a major shipping thoroughfare to and from the New World. The Florida Keys are now recognized as one of the great recreational and environmental resources of the United States. The islands are outposts of a laid-back, tropical resort culture that has as its foundation warmth and clear water. A significant part of the attraction is fishing, diving, and boating around the area's coral reefs, which the islands protect. But the reefs were not always so highly valued. The Florida Keys that have protected the reefs for millennia, may now be the source of the agents that may accomplish what Agassiz thought was beyond man's power a century ago.

  2. Phylogenetic analysis and expression patterns of tropomyosin in amphioxus%文昌鱼tropomyosin基因的克隆、进化分析及其胚胎发育与成体中的表达模式

    Institute of Scientific and Technical Information of China (English)

    李忻怡; 林浴霜; 张红卫

    2012-01-01

    In amphioxus, we found a mesoderm related gene, tropomyosin, which encodes a protein comprising 284 amino acid residues, sharing high identities with other known Tropomyosin proteins both in vertebrates and invertebrates. Phylogenetically, amphioxus Tropomyosin fell outside the invertebrate clade and was at the base of the vertebrate protein family clade, indicating that it may represent an independent branch. From the early neurula to the larva stage, whole-mount in situ hybridization and histological sections found transcripts of amphioxus tropomyosin gene. Weak tropomyosin expression was first detected in the wall of the archenteron at about 10 hours-post-fertilization neurula stage, while intense expression was revealed in the differentiating presumptive notochord and the muscle. Transcripts of tropomyosin were then expressed in the formed notochord and somites. Gene expression seemed to continue in these developing organs throughout the neurular stages and remained till 72-hours, during the early larval stages. In situ study still showed tropomyosin was also expressed in the neural tube, hepatic diverticulum, notochord and the spaces between myotomes in adult amphioxus. Our results indicated that tropomyosin may play an important role in both embryonic development and adult life.%Tropomyosin是一种分布广泛而且在进化上十分保守的蛋白,是肌肉形成和收缩过程中重要的调节蛋白质.通过RT-PCR和RACE技术得到文昌鱼tropomyosin基因全长,编码一个含284个氨基酸残基的蛋白质,将文昌鱼Tropomyosin和在其他物种中的同源物进行比对建树,发现其在功能域上高度保守并且只有一个拷贝,符合动物分类学中各物种的进化地位.胚胎整体原位杂交实验得知,tropomyosin在文昌鱼早期发育的表达,最早从原肠胚末期神经胚早期开始,定位于分化中的中内胚层.到神经胚期,tropomyosin的表达出现在发育中的体节和脊索中.随着发育的进

  3. Water withdrawals in Florida, 2012

    Science.gov (United States)

    Marella, Richard L.

    2015-09-01

    In 2012, the total amount of water withdrawn in Florida was estimated to be 14,237 million gallons per day (Mgal/d). Saline water accounted for 7,855 Mgal/d (55 percent), and freshwater accounted for 6,383 Mgal/d (45 percent). Groundwater accounted for 4,167 Mgal/d (65 percent) of freshwater withdrawals, and surface water accounted for the remaining 2,216 Mgal/d (35 percent). Surface water accounted for nearly all (99.9 percent) saline-water withdrawals. Freshwater withdrawals were greatest in Palm Beach County (682 Mgal/d), and saline-water withdrawals were greatest in Pasco County (1,822 Mgal/d). Fresh groundwater provided drinking water (through either public supply or private domestic wells) for 17.699 million residents (93 percent of Florida’s population), and fresh surface water provided drinking water for 1.375 million residents (7 percent). The statewide public-supply gross per capita water use for 2012 was estimated at 136 gallons per day.

  4. 2005 Southwest Florida Water Management District (SWFWMD) Lidar: Manatee District

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Light Detection and Ranging (LiDAR) LAS dataset is a survey of select areas within Southwest Florida. These data were produced for the Southwest Florida Water...

  5. Impact of immigration on health and human services: Florida's experience.

    Science.gov (United States)

    McNeece, C Aaron; Falconer, Mary Kay; Springer, David

    2002-01-01

    Florida has been the destination for large numbers of immigrants fleeing political persecution or economic hardships. Cubans and Haitians have been two of the largest immigrant groups arriving and settling in Florida. Both have received national and local attention. This article describes the immigration experience of Haitians and Cubans in Florida. The descriptions emphasize the differences between these two groups in their adjustment to life in south Florida. The article also addresses Florida's reaction to federal policies regarding immigration and highlights Florida's struggle to meet the service needs of these immigrant populations. Fiscal impacts of immigration are quantified in several service categories, including education, social services, health care, and criminal justice. Florida's action based on the documentation of the immigration fiscal impact is explained. Finally, how the state allocated the $18 million in federal funding provided as a response to Florida's documented impact is covered.

  6. West Florida shelf upwelling: Origins and pathways

    Science.gov (United States)

    Weisberg, Robert H.; Zheng, Lianyuan; Liu, Yonggang

    2016-08-01

    Often described as oligotrophic, the west Florida continental shelf supports abundant fisheries, experiences blooms of the harmful alga, Karenia brevis, and exhibits subsurface chlorophyll maxima evident in shipboard and glider surveys. Renewal of inorganic nutrients by the upwelling of deeper ocean water onto the shelf may account for this, but what are the origins and pathways by which such new water may broach the shelf break and advance toward the shoreline? We address these questions via numerical model simulations of pseudo-Lagrangian, isopycnic water parcel trajectories. Focus is on 2010, when the west Florida shelf was subjected to an anomalously protracted period of upwelling caused by Gulf of Mexico Loop Current interactions with the shelf slope. Origins and pathways are determined by integrating trajectories over successive 45 day intervals, beginning from different locations along the shelf break and at various locations and depths along the shelf slope. Waters upwelling across the shelf break are found to originate from relatively shallow depths along the shelf slope. Even for the anomalous 2010 year, much of this upwelling occurs from about 150 m and above, although waters may broach the shelf break from 300 m depth, particularly in the Florida Panhandle. Such interannual renewal of west Florida shelf waters appears to have profound effects on west Florida shelf ecology.

  7. Florida Sinkholes and Grout Injection Stabilization

    Directory of Open Access Journals (Sweden)

    Charles Hunt Griffith II

    2014-09-01

    Full Text Available Florida has a major problem when it comes to sinkholes. These sinkholes can become very hazardous to people, homes, and to the landscape as a whole. Florida sits on a carbonate platform which is highly indicative of sinkholes. There are three main types of sinkholes which occur in Florida: dissolution, cover subsidence, and cover collapse. I will compare these types of sinkholes to the underlying formation beneath Florida to see if there is a connection between the types of sinkholes that occur. I will also create a 3D model of grout injection stabilization and calculate its volume to compare to the actual volume placed under the house. This information will help inform and bring attention to the problem in Florida and in turn, may help alleviate the problem if we can understand what causes these sinkholes. The 3D model may help engineering companies become more efficient in predicting the projected amount of volume to stabilize a house that may be in danger.

  8. Water Use in Florida, 2005 and Trends 1950-2005

    Science.gov (United States)

    Marella, Richard L.

    2008-01-01

    Water is among Florida's most valued resources. The State has more than 1,700 streams and rivers, 7,800 freshwater lakes, 700 springs, 11 million acres of wetlands, and underlying aquifers yielding quantities of freshwater necessary for both human and environmental needs (Fernald and Purdum, 1998). Although renewable, these water resources are finite, and continued growth in population, tourism, and agriculture will place increased demands on these water supplies. The permanent population of Florida in 2005 totaled 17.9 million, ranking fourth in the Nation (University of Florida, 2006); nearly 86 million tourists visited the State (Orlando Business Journal, 2006). In 2005, Florida harvested two-thirds of the total citrus production in the United States and ranked fifth in the Nation net farm income (Florida Department of Agriculture and Consumer Services, 2006). Freshwater is vital for sustaining Florida's population, economy, and agricultural production. Accurate estimates reflecting water use and trends in Florida are compiled in 5-year intervals by the U.S. Geological Survey (USGS) in cooperation with the Florida Department of Environmental Protection (FDEP) and the Northwest Florida, St. Johns River, South Florida, Southwest Florida, and Suwannee River Water Management Districts (Marella, 2004). This coordinated effort provides the necessary data and information for planning future water needs and resource management. The purpose of this fact sheet is to present the highlights of water use in Florida for 2005 along with some significant trends in withdrawals since 1950.

  9. An Analysis of Florida Physical Educators' Knowledge of Bicycle Laws

    Science.gov (United States)

    Connaughton, Daniel P.; Egberts, John B.; Spengler, J. O.; Zhang, James J.; Jin, Liyan

    2012-01-01

    Bicycling among youth is a popular activity, but like all modes of travel it is not without risk. Florida has a particularly high rate of bicycle-related fatalities and injuries. To reduce such risks, the Florida Department of Transportation and Florida Department of Education have developed a youth bicycle safety educational program (Florida…

  10. 7 CFR 915.332 - Florida avocado maturity regulation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Florida avocado maturity regulation. 915.332 Section... SERVICE (Marketing Agreements and Orders; Fruits, Vegetables, Nuts), DEPARTMENT OF AGRICULTURE AVOCADOS GROWN IN SOUTH FLORIDA Container and Pack Regulations § 915.332 Florida avocado maturity regulation....

  11. 7 CFR 915.305 - Florida Avocado Container Regulation 5.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Florida Avocado Container Regulation 5. 915.305... AVOCADOS GROWN IN SOUTH FLORIDA Container and Pack Regulations § 915.305 Florida Avocado Container Regulation 5. (a) No handler shall handle any avocados for the fresh market from the production area to...

  12. Florida commercial space initiatives and technology transfer mechanisms

    Science.gov (United States)

    Moore, Roger L.

    1989-01-01

    This paper discusses commercial space policy for the State of Florida in the context of state initiatives for general technology and economic development. The paper also compares Florida's commercial space initiatives to national space policies and describes mechanisms for transferring space related technologies and research to Florida businesses for subsequent development and commercialization.

  13. Identifying Invasive Species Educational Needs in Florida: Opportunities for Extension

    Science.gov (United States)

    Huang, Pei-wen; Lamm, Alexa J.

    2016-01-01

    Florida's ecology has been adversely affected by invasive species. In Florida, a study was conducted to explore opportunities for Extension educators to contribute to combating the issue of invasive species. Florida residents' responses were captured through the use of an online public opinion survey. The findings revealed a need for invasive…

  14. Landscape Analysis of Adult Florida Panther Habitat.

    Directory of Open Access Journals (Sweden)

    Robert A Frakes

    Full Text Available Historically occurring throughout the southeastern United States, the Florida panther is now restricted to less than 5% of its historic range in one breeding population located in southern Florida. Using radio-telemetry data from 87 prime-aged (≥3 years old adult panthers (35 males and 52 females during the period 2004 through 2013 (28,720 radio-locations, we analyzed the characteristics of the occupied area and used those attributes in a random forest model to develop a predictive distribution map for resident breeding panthers in southern Florida. Using 10-fold cross validation, the model was 87.5 % accurate in predicting presence or absence of panthers in the 16,678 km2 study area. Analysis of variable importance indicated that the amount of forests and forest edge, hydrology, and human population density were the most important factors determining presence or absence of panthers. Sensitivity analysis showed that the presence of human populations, roads, and agriculture (other than pasture had strong negative effects on the probability of panther presence. Forest cover and forest edge had strong positive effects. The median model-predicted probability of presence for panther home ranges was 0.81 (0.82 for females and 0.74 for males. The model identified 5579 km2 of suitable breeding habitat remaining in southern Florida; 1399 km2 (25% of this habitat is in non-protected private ownership. Because there is less panther habitat remaining than previously thought, we recommend that all remaining breeding habitat in south Florida should be maintained, and the current panther range should be expanded into south-central Florida. This model should be useful for evaluating the impacts of future development projects, in prioritizing areas for panther conservation, and in evaluating the potential impacts of sea-level rise and changes in hydrology.

  15. Biodiversity in a Florida Sandhill Ecosystem

    Directory of Open Access Journals (Sweden)

    Samantha Robertson

    2009-01-01

    Full Text Available This project compares two transects of land in the University of South Florida's Botanical Gardens for their biodiversity. The transects were chosen to represent a Florida sandhill ecosystem and the individual Longleaf Pine, Saw Palmetto, Turkey Oak, Laurel Oak and Live Oak specimens were counted. All other species above waist height were counted as "other"?. Once the individuals were counted, the Simpson's and Shannon-Wiener indices were calculated. Since the Shannon-Wiener index incorporates several diversity characteristics, it is typically more reliable than Simpson's. However, both biodiversity indices agreed that transect B was more diverse than transect A.

  16. 2007 Florida Division of Emergency Management (FDEM) Lidar Project: Southwest Florida

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a topographic survey conducted for a coalition of GIS practitioners, including the Florida Division of...

  17. 2004 - 2008 Florida Division of Emergency Management (FDEM) Lidar Project: Southwest Florida

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a topographic survey conducted for a coalition of GIS practitioners, including the Florida Division of...

  18. Richard Florida : loovsektor on majanduskasvu mootor / Richard Florida ; interv. Argo Ideon

    Index Scriptorium Estoniae

    Florida, Richard

    2008-01-01

    USA majandus- ja ühiskonnateadlane Richard Florida loovklassi teooriast, selle osast majanduskasvu tagamisel, seosest ühiskonna tolerantsuse ja ühiskonna majandusliku edukuse vahel, sotsiaalse sidususe takistavast rollist loovuse motiveerimisel

  19. Florida Teachers' Attitudes about Teaching Evolution

    Science.gov (United States)

    Fowler, Samantha R.; Meisels, Gerry G.

    2010-01-01

    A survey of Florida teachers reveals many differences in comfort level with teaching evolution according to the state's science teaching standards, general attitudes and beliefs about evolution, and the extent to which teachers are criticized, censured, disparaged, or reprehended for their beliefs about the teaching of evolution.

  20. 77 FR 42352 - Florida Disaster #FL-00072

    Science.gov (United States)

    2012-07-18

    ... ADMINISTRATION Florida Disaster FL-00072 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... loan applications to: U.S. Small Business Administration, Processing and Disbursement Center, 14925... Assistance, U.S. Small Business Administration, 409 3rd Street SW., Suite 6050, Washington, DC...

  1. 78 FR 48763 - Florida Disaster #FL-00090

    Science.gov (United States)

    2013-08-09

    ... ADMINISTRATION Florida Disaster FL-00090 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY... completed loan applications to: U.S. Small Business Administration, Processing and Disbursement Center... Disaster Assistance, U.S. Small Business Administration, 409 3rd Street SW., Suite 6050, Washington,...

  2. Commercial Refrigeration Technology. Florida Vocational Program Guide.

    Science.gov (United States)

    University of South Florida, Tampa. Dept. of Adult and Vocational Education.

    The program guide for commercial refrigeration technology courses in Florida identifies primary considerations for the organization, operation, and evaluation of a vocational education program. Following an occupational description for the job title for refrigeration mechanic, and its Dictionary of Occupational Titles code, are six sections…

  3. Florida and Tennessee: Accountability in Civic Education

    Science.gov (United States)

    Delander, Brady

    2014-01-01

    While most states require testing in social studies or civic education, two states attach consequences for students and schools based on required statewide civics exams. Lawmakers in Florida, in 2010, and in Tennessee, in 2012, approved legislation that holds students accountable for their civics knowledge. Students are taking the tests for the…

  4. Sensation™ ‘Florida 127’ Strawberry

    Science.gov (United States)

    Florida127’ strawberry originated from a 2009 cross between Winterstar™ ‘FL 05-107’ (female parent) and unreleased selection FL 02-58 (male parent). It is a short-day genotype adapted to an annual plasticulture growing system. The plant is upright with open architecture, allowing air movement and e...

  5. USGS research on Florida's isolated freshwater wetlands

    Science.gov (United States)

    Torres, Arturo E.; Haag, Kim H.; Lee, Terrie M.; Metz, Patricia A.

    2011-01-01

    The U.S. Geological Survey (USGS) has studied wetland hydrology and its effects on wetland health and ecology in Florida since the 1990s. USGS wetland studies in Florida and other parts of the Nation provide resource managers with tools to assess current conditions and regional trends in wetland resources. Wetland hydrologists in the USGS Florida Water Science Center (FLWSC) have completed a number of interdisciplinary studies assessing the hydrology, ecology, and water quality of wetlands. These studies have expanded the understanding of wetland hydrology, ecology, and related processes including: (1) the effects of cyclical changes in rainfall and the influence of evapotranspiration; (2) surface-water flow, infiltration, groundwater movement, and groundwater and surfacewater interactions; (3) the effects of water quality and soil type; (4) the unique biogeochemical components of wetlands required to maintain ecosystem functions; (5) the effects of land use and other human activities; (6) the influences of algae, plants, and invertebrates on environmental processes; and (7) the effects of seasonal variations in animal communities that inhabit or visit Florida wetlands and how wetland function responds to changes in the plant community.

  6. Ask a Librarian: Florida's Virtual Reference Service

    Science.gov (United States)

    Sachs, Diana

    2004-01-01

    Florida's Ask a Librarian service (http://www.askalibrarian.org) brings virtual reference to users at their moment of need via the Internet. Ask a Librarian is a growing service with 76 participating libraries including public, school, four-year, and community college libraries. The following article describes how Ask a Librarian was developed…

  7. AECT Convention, Orlando, Florida 2008 Report

    Science.gov (United States)

    Vega, Eddie

    2009-01-01

    This article presents several reports that highlight the events at the 2008 Association for Educational Communications and Technology (AECT) International Convention in Orlando, Florida. At the annual convention this year, the Multimedia Production Division goal was to continue to share information about the latest tools in multimedia production,…

  8. Black Frontier Settlements in Spanish Colonial Florida.

    Science.gov (United States)

    Landers, Jane

    1988-01-01

    Addresses the much neglected area of Black frontier experience in the Spanish colonies. Concentrates on the role played by Black settlers and one Black township in defending the Spanish frontier in colonial Florida against the threat of growing English settlements to the north. Provides an introduction to the 18th century Southeastern Spanish…

  9. Endangered Species of Florida Coloring Book.

    Science.gov (United States)

    King, Merlien W.

    This coloring book portrays endangered animal and plant species of Florida in their natural environment. Each picture is to be colored by the student. On the back of each page bearing the picture to be colored is a description of the animal or plant, its preferred habitat, and the reason the animal or plant is endangered. (RE)

  10. 77 FR 66083 - Florida Disaster # FL-00076

    Science.gov (United States)

    2012-11-01

    ... ADMINISTRATION Florida Disaster FL-00076 AGENCY: U.S. Small Business Administration. ACTION: Notice. SUMMARY: This is a Notice of the Presidential declaration of a major disaster for Public Assistance Only for the... Kingsport Road, Fort Worth, TX 76155. FOR FURTHER INFORMATION CONTACT: A. Escobar, Office of...

  11. Environmental Citizenship in Florida's Middle Schools.

    Science.gov (United States)

    Florida State Dept. of Environmental Protection, Tallahassee.

    This brochure introduces Environmental Citizenship in Florida's Middle Schools, which is a school-wide exercise aimed at making middle school students more aware of the natural world around them and their effect on that world. As many school-study areas as possible are used to help students learn how the environment can affect all aspects of their…

  12. Conservation of the glucan phosphatase laforin is linked to rates of molecular evolution and the glucan metabolism of the organism

    Directory of Open Access Journals (Sweden)

    Pace Rachel M

    2009-06-01

    Full Text Available Abstract Background Lafora disease (LD is a fatal autosomal recessive neurodegenerative disease. A hallmark of LD is cytoplasmic accumulation of insoluble glucans, called Lafora bodies (LBs. Mutations in the gene encoding the phosphatase laforin account for ~50% of LD cases, and this gene is conserved in all vertebrates. We recently demonstrated that laforin is the founding member of a unique class of phosphatases that dephosphorylate glucans. Results Herein, we identify laforin orthologs in a protist and two invertebrate genomes, and report that laforin is absent in the vast majority of protozoan genomes and it is lacking in all other invertebrate genomes sequenced to date. We biochemically characterized recombinant proteins from the sea anemone Nematostella vectensis and the amphioxus Branchiostoma floridae to demonstrate that they are laforin orthologs. We demonstrate that the laforin gene has a unique evolutionary lineage; it is conserved in all vertebrates, a subclass of protists that metabolize insoluble glucans resembling LBs, and two invertebrates. We analyzed the intron-exon boundaries of the laforin genes in each organism and determine, based on recently published reports describing rates of molecular evolution in Branchiostoma and Nematostella, that the conservation of laforin is linked to the molecular rate of evolution and the glucan metabolism of an organism. Conclusion Our results alter the existing view of glucan phosphorylation/dephosphorylation and strongly suggest that glucan phosphorylation is a multi-Kingdom regulatory mechanism, encompassing at least some invertebrates. These results establish boundaries concerning which organisms contain laforin. Laforin is conserved in all vertebrates, it has been lost in the vast majority of lower organisms, and yet it is an ancient gene that is conserved in a subset of protists and invertebrates that have undergone slower rates of molecular evolution and/or metabolize a carbohydrate

  13. Unexpected multiplicity of QRFP receptors in early vertebrate evolution.

    Science.gov (United States)

    Larhammar, Dan; Xu, Bo; Bergqvist, Christina A

    2014-01-01

    The neuropeptide QRFP, also called 26RFa, and its G protein-coupled receptor GPR103 have been identified in all vertebrates investigated. In mammals, this peptide-receptor pair has been found to have several effects including stimulation of appetite. Recently, we reported that a QRFP peptide is present in amphioxus, Branchiostoma floridae, and we also identified a QRFP receptor (QRFPR) that mediates a functional response to sub-nanomolar concentrations of the amphioxus peptide as well as short and long human QRFP (Xu et al., submitted). Because the ancestral vertebrate underwent two tetraploidizations, it might be expected that duplicates of the QRFP gene and its receptor gene may exist. Indeed, we report here the identification of multiple vertebrate QRFPR genes. Three QRFPR genes are present in the coelacanth Latimeria chalumnae, representing an early diverging sarcopterygian lineage. Three QRFPR genes are present in the basal actinopterygian fish, the spotted gar. Phylogenetic and chromosomal analyses show that only two of these receptor genes are orthologous between the two species, thus demonstrating a total of four distinct vertebrate genes. Three of the QRFPR genes resulted from the early vertebrate tetraploidizations and were copied along with syntenic neuropeptide Y receptor genes. The fourth QRFPR gene may be an even older and distinct lineage. Because mammals and birds have only a single QRFPR gene, this means that three genes have been lost in these lineages, and at least one of these was lost independently in mammals and birds because it is still present in a turtle. In conclusion, these results show that the QRFP system gained considerable complexity in the early stages of vertebrate evolution and still maintains much of this in some lineages, and that it has been secondarily reduced in mammals.

  14. Unexpected multiplicity of QRFP receptors in early vertebrate evolution

    Directory of Open Access Journals (Sweden)

    Dan eLarhammar

    2014-10-01

    Full Text Available The neuropeptide QRFP, also called 26RFa, and its G protein-coupled receptor GPR103 have been identified in all vertebrates investigated. In mammals, this peptide-receptor pair has been found to have several effects including stimulation of appetite. Recently, we reported that a QRFP peptide is present in amphioxus, Branchiostoma floridae, and we also identified a QRFP receptor (QRFPR that mediates a functional response to sub-nanomolar concentrations of the amphioxus peptide as well as short and long human QRFP (Xu et al., submitted. Because the ancestral vertebrate underwent two tetraploidizations, it might be expected that duplicates of the QRFP gene and its receptor gene may exist. Indeed, we report here the identification of multiple vertebrate QRFPR genes. Three QRFPR genes are present in the coelacanth Latimeria chalumnae, representing an early diverging sarcopterygian lineage. Three QRFP receptor genes are present in the basal actinopterygian fish, the spotted gar. Phylogenetic and chromosomal analyses show that only two of these receptor genes are orthologous between the two species, thus demonstrating a total of four distinct vertebrate genes. Three of the QRFPR genes resulted from the early vertebrate tetraploidizations and were copied along with syntenic neuropeptide Y receptor genes. The fourth QRFPR gene may be an even older and distinct lineage. Because mammals and birds have only a single QRFP receptor gene, this means that three genes have been lost in these lineages, and at least one of these was lost independently in mammals and birds because it is still present in a turtle. In conclusion, these results show that the QRFP system gained considerable complexity in the early stages of vertebrate evolution and still does so in some lineages, and that it has been secondarily reduced in mammals.

  15. Staghorn tempestites in the Florida Keys

    Science.gov (United States)

    Shinn, E.A.; Reich, C.D.; Hickey, T.D.; Lidz, B.H.

    2003-01-01

    Thirty-one samples of transported Holocene Acropora cervicornis "sticks" sampled from carbonate sand tempestite accumulations at 19 sites along a 180-km-long stretch of the Florida reef tract were dated using the radiocarbon (14C) method. The "modern fossils" collected from just a few centimeters below the surface ranged in age from 0.5 to 6.4 ka. The majority lived between 3.5 and 5.5 ka. The time of transport and deposition is not known. There were no A. cervicornis samples centered around 4.5 ka. Acropora cervicornis is living on many Florida reefs, but the youngest tempestite sample was 500 years old. Two 500-year-long gaps in dated staghorn suggest that the documented decline in living A. cervicornis over the past 25 years may not be without precedent.

  16. Florida Law Enforcement’s Role in Agroterrorism

    Science.gov (United States)

    2014-12-01

    region offices in Orlando, Pensacola, and West Palm Beach.204 Of the six task forces, the South Florida JTTF is in the largest of the states urban...bacterial disease was found infecting trees in Miami. The canker quickly began spread across South Florida infecting 24 counties. With Florida’s citrus...areas, Miami. The South Florida JTTF, as described by the FBI Miami Division, “brings together representatives of local, state, and federal agencies to

  17. Agkistrodon piscivorus conanti (Florida cottonmouth) Diet

    Science.gov (United States)

    Grajal-Puche, Alejandro; Josimovich, Jillian; Falk, Bryan; Reed, Robert

    2016-01-01

    Agkistrodon piscivorus is a generalist predator that feeds on a variety of prey, including snakes (Gloyd and Conant 1990. Snakes of the Agkistrodon Complex: A Monographic Review. Society for the Study of Amphibians and Reptiles, Oxford, Ohio. 614 pp.; Lillywhite et al. 2002. Herpetol. Rev. 33:259–260; Hill and Beaupre 2008. Copeia 2008:105–114). Cemophora coccinea (Scarletsnake) is not known as one of the 26 species of snakes consumed by A. piscivorus (Ernst and Ernst 2011. Venomous Reptiles of the United States, Canada, and Northern Mexico: Volume 1. Johns Hopkins University Press, Baltimore, Maryland. 193 pp.). On 16 June 2015, at 2210 h, we found a dead-on-road A. piscivorus (total length [TL] = 51.0 cm) in Everglades National Park on Main Park Road, 1.88 km S Pa-hay-okee, Miami-Dade Co., Florida, USA (25.414085°N, 80.78183146°W, WGS84; elev. 3 m). The snake had been killed by a vehicle and some internal organs were exposed. Visible stomach contents included a small (TL ca. 15 cm) C. coccinea. Photographic vouchers of the A. piscivorus (UF-Herpetology 177194) and C. coccinea (UF-Herpetology 177195) were deposited in the Division of Herpetology, Florida Museum of Natural History, University of Florida. Despite the fact that these species are sympatric over large areas of the southeastern United States, this is the first known documented predation of C. coccinea by A. piscivorus.

  18. Masticophis flagellum selects florida scrub habitat at multiple spatial scales

    Science.gov (United States)

    Halstead, B.J.; Mushinsky, H.R.; McCoy, E.D.

    2009-01-01

    The use of space by individual animals strongly influences the spatial extent, abundance, and growth rates of their populations. We analyzed the spatial ecology and habitat selection of Masticophis flagellum (the coachwhip) at three different scales to determine which habitats are most important to this species. Home ranges and mean daily displacements of M. flagellum in Florida were large compared to individuals in other populations of this species. Home ranges contained a greater proportion of Florida scrub habitat than did the study site as a whole, and individuals selected Florida scrub habitat within their home ranges. For both selection of the home range within the study site and selection of habitats within the home range, mesic cutthroat and hydric swamp habitats were avoided. Standardized selection ratios of Florida scrub patches were positively correlated with lizard abundance. Several non-mutually exclusive mechanisms, including foraging success (prey abundance, prey vulnerability, and foraging efficiency), abundance of refugia, and thermoregulatory opportunity may underlie the selection of Florida scrub by M. flagellum. Historic rarity and anthropogenic loss and fragmentation of Florida scrub habitat, coupled with the long-distance movements, large home ranges, and selection of Florida scrub by M. flagellum, indicate that large contiguous tracts of land containing Florida scrub will be essential for the persistence of M. flagellum in central Florida. ?? 2009 by The Herpetologists' League, Inc.

  19. 2012 Southwest Florida Water Management District (SWFWMD) Lidar: Lake Manatee

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Geographic Information System (GIS). Southwest Florida Water Management District (SWFWMD) regularly uses digital topographic information to support regulatory, land...

  20. FL State Profile. Florida: Florida Comprehensive Assessment Test[R] (FCAT)

    Science.gov (United States)

    Center on Education Policy, 2010

    2010-01-01

    This paper provides information about the Florida Comprehensive Assessment Test[R] (FCAT). The purpose of the exam is to: (1) Determine prospective high school graduates' mastery of the state curriculum; (2) Encourage districts and schools to identify and serve students at risk of academic failure; (3) Provide data to state policymakers on student…

  1. California avocados in Florida? Finding the perfect avocado for production in East-Central Florida

    Science.gov (United States)

    Avocado (Persea americana Mill.) is a high-value fruit where most U.S. consumption is supplied using imported product. Cultivars with good fruit quality and horticultural traits may provide a useful alternative crop in east-central Florida and possibly in other locations throughout the state. A port...

  2. Community Resources Guide for Central Florida = Una Guia de Recursos en la Comunidad de Florida Central.

    Science.gov (United States)

    Medina, Linda, Comp.

    Designed to orient Hispanic refugees to the services that are available in Central Florida, this bilingual guide consists of a section of general information on living and working in the United States and a section devoted to various public and private agencies. Provided first are addresses and phone numbers of various government agencies:…

  3. Successful biological control of tropical soda apple in Florida

    Science.gov (United States)

    Tropical soda apple, Solanum viarum, is a small shrub native to tropical regions of Brazil, Paraguay, and Argentina. This weed was first found in Florida in 1988. In May 2003, a leaf feeding beetle, Gratiana boliviana, from South America was released in Florida as a biological control agent of tro...

  4. In the Eye of the Beholder: Waterhyacinth Management in Florida

    Science.gov (United States)

    In Florida, herbicides are the primary tactic employed to control waterhyacinth, Eichhornia crassipes with little to no consideration given to the presence of the three biological control agents introduced intentionally during the 1970's. Field research conducted at four Florida sites quantified th...

  5. Avocado pests in Florida: Not what you expected

    Science.gov (United States)

    Avocado, Persea americana Mill., is Florida's second most important fruit crop after citrus. Until recently, the complex of spider mite and insect pests that affected avocado in south Florida was under a 20 year Integrated Pest Management (IPM) program. The recent invasion of avocado orchards by a...

  6. 76 FR 38592 - Phosphorus Water Quality Standards for Florida Everglades

    Science.gov (United States)

    2011-07-01

    ... AGENCY 40 CFR Part 131 Phosphorus Water Quality Standards for Florida Everglades AGENCY: Environmental... provisions of Florida's Water Quality Standards for Phosphorus in the Everglades Protection Area (Phosphorus... are not applicable water quality standards for purposes of the Clean Water Act. EPA is proposing...

  7. 77 FR 46298 - Phosphorus Water Quality Standards for Florida Everglades

    Science.gov (United States)

    2012-08-03

    ... AGENCY 40 CFR Part 131 RIN 2040-AF38 Phosphorus Water Quality Standards for Florida Everglades AGENCY... provisions of Florida's Water Quality Standards for Phosphorus in the Everglades Protection Area (Phosphorus... are not applicable water quality standards for purposes of the Clean Water Act. EPA is...

  8. 7 CFR 1006.2 - Florida marketing area.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 9 2010-01-01 2009-01-01 true Florida marketing area. 1006.2 Section 1006.2 Agriculture Regulations of the Department of Agriculture (Continued) AGRICULTURAL MARKETING SERVICE (Marketing Agreements and Orders; Milk), DEPARTMENT OF AGRICULTURE MILK IN THE FLORIDA MARKETING AREA Order...

  9. Florida exotic whitefly invaders from the last decade

    Science.gov (United States)

    The state of Florida hosts a large number of exotic species with many new “invasives” arriving annually. Among invasive insects establishing in Florida over the past decade are three whiteflies (Hemiptera: Aleyrodidae) that cause highly visible wax and sooty mold buildup in urban plantings as well a...

  10. Physical Characterization of Florida International University Simulants

    Energy Technology Data Exchange (ETDEWEB)

    HANSEN, ERICHK.

    2004-08-19

    Florida International University shipped Laponite, clay (bentonite and kaolin blend), and Quality Assurance Requirements Document AZ-101 simulants to the Savannah River Technology Center for physical characterization and to report the results. The objectives of the task were to measure the physical properties of the fluids provided by FIU and to report the results. The physical properties were measured using the approved River Protection Project Waste Treatment Plant characterization procedure [Ref. 1]. This task was conducted in response to the work outlined in CCN066794 [Ref. 2], authored by Gary Smith and William Graves of RPP-WTP.

  11. Management case study: Tampa Bay, Florida

    Science.gov (United States)

    Morrison, G.; Greening, H.S.; Yates, K.K.

    2012-01-01

    Tampa Bay, Florida,USA, is a shallow,subtropical estuary that experienced severe cultural eutrophication between the 1940s and 1980s, a period when the human population of its watershed quadrupled. In response, citizen action led to the formation of a public- and private-sector partnership (the Tampa Bay Estuary Program), which adopted a number of management objectives to support the restoration and protection of the bay’s living resources. These included numeric chlorophyll a and water-clarity targets, as well as long-term goals addressing the spatial extent of sea grasses and other selected habitat types, to support estuarine-dependent faunal guilds.

  12. Enfermedades del Aguacate en La Florida

    Directory of Open Access Journals (Sweden)

    Stevens H. E.

    1942-04-01

    Full Text Available El aguacate cultivado bajo las condiciones ambienciales de La Florida, está sujeto a ser atacado por varias enfermedades parasitarias. Algunas de estas son de menor importancia y no requieren métodos de represión especiales; otras al contrario son más severas y requieren atención cada año; y otras además están sujetas a las condiciones climatológicas, y su intensidad dependerá principalmente de las condiciones meteorológicas durante algunos períodos críticos en el crecimiento del árbol.

  13. Prescribed Fire is Cool on Florida Highway

    OpenAIRE

    Caster, Jeff; McBurney, Willson; Farley, Patricia; Rodriguez, Rose; Green, Lane; McGorty, Kevin

    2007-01-01

    Though unprecedented in the sunshine state, plans for a prescribed fire on US319/SR61, Kate Ireland Parkway in north Florida sparked enthusiasm and excitement among roadside managers. The recently expanded high speed corridor passes for ten miles through the Red Hills Region (www.ttrs.org/rhcp); a rural landscape that is host to America’s largest remnant of the great longleaf pine forest (www.longleafalliance.org). Prescribed fire is a necessary and popular landscape management tool used by g...

  14. The surficial aquifer in Pinellas County, Florida

    Science.gov (United States)

    Causseaux, K.W.

    1985-01-01

    The surficial aquifer in Pinellas County, Florida, contains potable water throughout most of the county and is a potential source of water to augment the public supply that is presently imported from adjacent counties. The county accounts for 38 percent of the public supply consumption of ground water in the 11-county area of west-central Florida and 68 percent of this water is imported from two adjacent counties. The surficial aquifer has a saturated thickness of more than 30 feet throughout most of the county. Specific capacity per foot of screen for wells is less than 0.1 gallon per minute per foot of drawdown in some parts of the county, but yield is sufficient in most of the county for many small uses with shallow-well pumps. Minimum potential yield varies from 5 gallons per minute in the northern part of the county to more than 30 gallons per minute in the south. Concentrations of iron are high enough in parts of the county to cause staining. Chloride concentrations are less than 100 milligrams per liter in most of the county and do not pose a problem for many uses. (USGS)

  15. History and Status of Eucalyptus Improvement in Florida

    Directory of Open Access Journals (Sweden)

    Donald L. Rockwood

    2012-01-01

    Full Text Available The first organized Eucalyptus research in Florida was begun by the Florida Forests Foundation in 1959 in southern Florida. This research was absorbed by the USDA Forest Service and the Florida Division of Forestry in 1968. In the early 1970s, the Eucalyptus Research Cooperative formed to provide additional support emphasized E. grandis, E. robusta, E. camaldulensis, and E. tereticornis and developed cultural practices for commercial plantations in southern Florida. In 1978, this cooperative united with the Hardwood Research Cooperative at North Carolina State University until 1985 when the 14-year effort ended after three severe freezes from 1983 to 1985. Eucalyptus planting and research were continued with a Florida-wide focus by the University of Florida and collaborators starting in 1980. The collective accomplishments in terms of genetic resources and commercial planting are summarized. For example, fast-growing, freeze-resilient E. grandis seedlings are produced by advanced generation seed orchards, five E. grandis cultivars are commercially available, as are E. amplifolia and Corymbia torelliana seeds. Genetic improvement of these and other species is ongoing due to beneficial collaborations. Short Rotation Woody Crop systems are promising for increasing productivity and extending uses beyond conventional pulpwood to applications such as windbreaks, dendroremediation, and energy wood.

  16. ASK Florida; a climate change education professional development program for middle school teachers in Florida

    Science.gov (United States)

    Weihs, R. R.

    2012-12-01

    A series of professional development workshops covering the fundamentals of climate change have been developed and facilitated for two groups of middle school science teachers in three Florida counties. The NASA-supported joint venture between Florida State University's Center for Ocean-Atmospheric Prediction Studies (COAPS) and the University of South Florida's (USF's) Coalition for Science Literacy, ASK Florida, focuses on expanding and deepening teachers' content knowledge of a wide range of climate change topics, connecting local and regional changes to the global picture, and supporting classroom implementation and effective teaching practices. Education experts from USF, climate scientists from COAPS, and Hillsborough county teachers and science coaches coordinated and developed the workshop content, which is based on Florida's Next Generation Sunshine State Standards in science, science curriculum guides for 6th grade, and teacher interest. Several scientists have facilitated activities during the workshop, including professors in meteorology and climatology, research scientists in the field, a NOAA program manager, the state climatologists for Florida, and others. Having these climate scientists present during the workshop provides teachers an opportunity to interact directly with the scientists and gain insight into the climatology field. Additionally, we host an open-forum discussion panel during which teachers can ask the experts about any topics of interest. Activities are designed to enhance the scientific skill level of the teachers. Introductory activities reinforce teachers' abilities to distinguish facts from opinions and to evaluate sources. Other activities provide hands-on experience using actual scientific data from NASA and other agencies. For example, teachers analyze precipitation data to create distributions of Florida rainfall, examine sea level trends at various locations, identify Atlantic hurricane frequencies during the phases of ENSO

  17. Florida's outpatient commitment law: a lesson in failed reform?

    Science.gov (United States)

    Petrila, John; Christy, Annette

    2008-01-01

    An involuntary outpatient commitment law became effective in Florida in January 2005. However, only 71 orders for outpatient commitment have been issued in three years, even though during that period 41,997 adults had two or more 72-hour involuntary emergency examinations under Florida's civil commitment law. This column describes the criteria for outpatient commitment in the Florida statute and discusses possible reasons for its low rate of use, including additional statutory criteria that make filing a petition for outpatient commitment difficult, lack of community treatment resources, and lack of enforcement mechanisms.

  18. Integrated Science: Florida Manatees and Everglades Hydrology

    Science.gov (United States)

    Langtimm, Catherine A.; Swain, Eric D.; Stith, Bradley M.; Reid, James P.; Slone, Daniel H.; Decker, Jeremy; Butler, Susan M.; Doyle, Terry; Snow, R.W.

    2009-01-01

    Predicting and monitoring restoration effects on Florida manatees, which are known to make extended movements, will be incomplete if modeling and monitoring are limited to the smaller areas defined by the various res-toration components. U.S. Geological Survey (USGS) efforts, thus far, have focused on (1) collecting manatee movement data throughout the Ten Thousand Islands (TTI) region, and (2) developing an individual-based model for manatees to illustrate manatee responses to changes in hydrology related to the Picayune Strand Restoration Project (PSRP). In 2006, new regional research was begun to extend an Everglades hydrology model into the TTI region; extend the manatee movement model into the southern estuaries of Everglades National Park (ENP); and integrate hydrology and manatee data, models, and monitoring across the TTI region and ENP. Currently (2008), three research tasks are underway to develop the necessary modeling components to assess restoration efforts across the Greater Everglades Ecosystem.

  19. Cestrum diurnum poisoning in Florida cattle.

    Science.gov (United States)

    Krook, L; Wasserman, R H; McEntee, K; Brokken, T D; Teigland, M B

    1975-10-01

    Cestrum diurnum poisoning was described in a Florida bull. Clinical signs included chronic wasting and progressive lameness. Plasma calcium was elevated for long periods of time but decreased toward low normal values. There was pronounced C-cell hyperplasia. Osteopetrosis was very severe and reflected retarded osteocytic osteolysis and chondrolysis. Further negative effects on the osteocytes eventually lead to osteonecrosis. Soft tissue calcinosis involved tendons and ligaments, major arteries and veins but kidneys and lungs were spared. Whereas the osteopetrosis could be explained by hypercalcitoninism, the osteonecrosis was believed to result from direct action by the Cestrum diurnum factor, previously shown to have an action similar to that of 1,25-dihydroxy-cholecalciferol, which is the biologically active metabolite of vitamin D3.

  20. The American Crocodile in Biscayne Bay, Florida

    Science.gov (United States)

    Cherkiss, Michael S.; Romañach, Stephanie S.; Mazzotti, Frank J.

    2011-01-01

    Intensive crocodile monitoring programs conducted during the late 1970s and early 1980s in southern Florida resulted in an optimistic outlook for recovery of the protected species population. However, some areas with suitable crocodile habitat were not investigated, such as Biscayne Bay and the mainland shorelines of Barnes and Card Sounds. The objective of our study was to determine status and habitat use of crocodiles in the aforementioned areas. Spotlight and nesting surveys were conducted from September 1996 to December 2005. The results revealed annual increases in the number of crocodiles. Crocodiles preferred protected habitats such as canals and ponds. Fewer crocodiles were observed in higher salinity water. The distribution and abundance of crocodilians in estuaries is directly dependent on timing, amount, and location of freshwater delivery, providing an opportunity to integrate habitat enhancement with ongoing ecosystem restoration and management activities.

  1. Worker exposure to ethion in Florida citrus.

    Science.gov (United States)

    Wojeck, G A; Nigg, H N; Stamper, J H; Bradway, D E

    1981-11-01

    Dermal and respiratory exposure to ethion was determined for 17 men in eight spray crews in Florida citrus spray operations. Respiratory exposure was less than 1% of the total exposure. Hands represented 42% of the total body exposure for applicators and 76% for suppliers. At one location, suppliers exhibited a larger decrease in ChE activity than applicators. This difference appeared related to the higher mean dermal ethion exposure to suppliers. Acute symptoms of organophosphorous poisoning were not observed. The total percent/hr of the probable human dermal LD50 was very low in all cases. These data indicate a relatively low potential acute hazard to workers applying ethion with air blast equipment under the conditions of this study.

  2. Hotel Polynesian Village Florida- (EE.UU.

    Directory of Open Access Journals (Sweden)

    Welton Becket y Asociados, Arquitectos

    1974-06-01

    Full Text Available This hotel unit with 500 rooms is located in a beautiful plot situated along the beach of a laguna in Disneyland, Florida. It consists of 10 individual pavilions, two units with two stories and six with three stories, in addition to a main building with two stories —offices, W.C.s, bar shops, banquet halls, etc.— and a monorail station connected with the covered passages, and with a view of the beautiful horizon that reminds one of the South Sea in the last century. The building for the guests is formed by metal structure modules of 9.10 x 4.50 x 2.70 m, that are placed on top of each other. Each one weighs 7 t and they are all completely equipped.Este conjunto hotelero, de 500 habitaciones, se halla enclavado en un hermoso solar situado a lo largo de la playa de una laguna del Mundo de Walt Disney, en Florida. Consta de diez pabellones individuales, dos unidades de dos plantas y seis de tres alturas, además de un edificio principal de dos plantas —oficinas, servicios, bar, tiendas, salones de banquetes, etc.— y una estación del monorraíl enlazada con los paseos cubiertos con vistas al bello horizonte, que recuerda el de los Mares del Sur, en el siglo pasado. Los edificios para huéspedes están formados por módulos de 9,14 X 4,57 X 2,74 m, con estructura metálica, que se colocan uno sobre otro. Cada uno pesa 7 t y van completamente equipados con todas sus instalaciones necesarias.

  3. EAARL Coastal Topography-Cape Canaveral, Florida, 2009: First Surface

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A digital elevation model (DEM) of a portion of the eastern Florida coastline was produced from remotely sensed, geographically referenced elevation measurements...

  4. 2006 Southwest Florida Water Management District (SWFWMD) Lidar: North District

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is one component of a digital terrain model (DTM) for the Southwest Florida Water Management District's FY2006 Digital Orthophoto (B089) and LiDAR...

  5. Accelerated sea level rise and Florida Current transport

    Directory of Open Access Journals (Sweden)

    J. Park

    2015-07-01

    Full Text Available The Florida Current is the headwater of the Gulf Stream and is a component of the North Atlantic western boundary current from which a geostrophic balance between sea surface height and mass transport directly influence coastal sea levels along the Florida Straits. A linear regression of daily Florida Current transport estimates does not find a significant change in transport over the last decade; however, a nonlinear trend extracted from empirical mode decomposition (EMD suggests a 3 Sv decline in mean transport. This decline is consistent with observed tide gauge records in Florida Bay and the straits exhibiting an acceleration of mean sea level (MSL rise over the decade. It is not known whether this recent change represents natural variability or the onset of the anticipated secular decline in Atlantic meridional overturning circulation (AMOC; nonetheless, such changes have direct impacts on the sensitive ecological systems of the Everglades as well as the climate of western Europe and eastern North America.

  6. Accelerated sea level rise and Florida Current transport

    Directory of Open Access Journals (Sweden)

    J. Park

    2015-04-01

    Full Text Available The Florida Current is the headwater of the Gulf Stream and is a component of the North Atlantic western boundary current from which a geostrophic balance between sea surface height and mass transport directly influence coastal sea levels along the Florida Straits. A linear regression of daily Florida Current transport estimates does not find a significant change in transport over the last decade, however, a nonlinear trend extracted from empirical mode decomposition suggests a 3 Sv decline in mean transport. This decline is consistent with observed tide gauge records in Florida Bay and the Straits, all exhibiting an acceleration of mean sea level rise over the decade. It is not known whether this recent change represents natural variability or the onset of the anticipated secular decline in Atlantic meridional overturning circulation, nonetheless, such changes have direct impacts on the sensitive ecological systems of the Everglades as well as the climate of western Europe and eastern North America.

  7. Environmental Contaminants Evaluation of St. Andrew Bay, Florida: Volume 1

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Between 1985 and 1997, a general survey of St. Andrew Bay, Florida, was conducted to measure chemical contaminant concentrations in the sediments and selected biota....

  8. Architecture of the Florida Power Grid as a Complex Network

    CERN Document Server

    Xu, Yan; Rikvold, Per Arne

    2013-01-01

    We study the Florida high-voltage power grid as a technological network embedded in space. Measurements of geographical lengths of transmission lines, the mixing of generators and loads, the weighted clustering coefficient, as well as the organization of edge conductance weights show a complex architecture quite different from random-graph models usually considered. In particular, we introduce a parametrized mixing matrix to characterize the mixing pattern of generators and loads in the Florida Grid, which is intermediate between the random mixing case and the semi-bipartite case where generator-generator transmission lines are forbidden. Our observations motivate an investigation of optimization (design) principles leading to the structural organization of power grids. We thus propose two network optimization models for the Florida Grid as a case study. Our results show that the Florida Grid is optimized not only by reducing the construction cost (measured by the total length of power lines), but also throug...

  9. Väiksemad klassid tekitavad Florida osariigis raskusi / Kaivo Kopli

    Index Scriptorium Estoniae

    Kopli, Kaivo

    2002-01-01

    Otsus vähendada klassides õpilaste arvu tekitab Floridas probleeme, sest ei piisa õpetajaid. Lisaks rahvaarvu kasvule on õpetajaskond vananenud ja suur osa neist läheb lähiaastail pensionile. Eriti raske on saada mehi õpetajaiks

  10. Daytona Beach, Florida Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Daytona Beach, Florida Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model....

  11. [Florida Panther National Wildlife Refuge: Comprehensive Conservation Plan]: Compatibility Determination

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document states that the actions proposed in the 2000 Florida Panther NWR Comprehensive Conservation Plan are compatible with Refuge goals.

  12. FLORIDA "STATE" MIGRANT HEALTH PROJECT, ANNUAL PROGRESS REPORT 1964 - 1965.

    Science.gov (United States)

    Florida State Board of Health, Jacksonville.

    THE REPORT DISCUSSES THE HOUSING, HEALTH SERVICES, SANITATION, AND HEALTH EDUCATION PROGRAMS FOR MIGRANT AGRICULTURAL WORKERS IN FLORIDA. IT STATES THE OBJECTIVES OF EACH PROGRAM, PROGRAM ACCOMPLISHMENTS DURING THE YEAR, AND SUGGESTIONS FOR FUTURE PROGRAMS. (CL)

  13. Gadsden, a Florida County in Word and Picture

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Company A of the Fourth Florida Infantry was mustered into Confederate service at Fort Mallory on St. Vincent's Island. Most of their first year of service was spent...

  14. POPS IN ALLIGATOR LIVERS FROM LAKE APOPKA, FLORIDA, USA

    Science.gov (United States)

    Reproductive disorders in American alligators (Alligator mississippiensis) inhabiting Lake Apopka, Florida, have been observed for several years. Such disorders are hypothesized to be caused by endocrine disrupting contaminants occurring in the Lake due to pesticide spills and ...

  15. Water-management models in Florida from LANDSAT-1 data

    Science.gov (United States)

    Higer, A. L.; Cordes, E. H.; Coker, A. E.; Rogers, R. H.

    1977-01-01

    ERTS-1 is described as a near real time, data relay system for south Florida water quantity and quality monitoring. An ecological model of the Shark River Slough in Everglades National Park is also presented.

  16. Key West, Florida Tsunami Forecast Grids for MOST Model

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Key West, Florida Forecast Model Grids provides bathymetric data strictly for tsunami inundation modeling with the Method of Splitting Tsunami (MOST) model. MOST...

  17. Thrips-transmitted Viruses Infect a Number of Florida Crops

    Science.gov (United States)

    The ilarviruses Tomato necrotic streak virus and Tobacco streak virus are present in south Florida. Both species cause economically significant disease in vegetable crop. Control of these viruses makes use of integrated pest management approaches....

  18. The MAFLA (Mississippi, Alabama, Florida) Study, Grain Size Analyses

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The MAFLA (Mississippi, Alabama, Florida) Study was funded by NOAA as part of the Outer Continental Shelf Program. Dr. L.J. Doyle produced grain size analyses in the...

  19. 2007 Northwest Florida Water Management District (NWFWMD) Lidar: Holmes County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — LIDAR-derived binary (.las) files containing points classified as bare-earth and canopy (first return) were produced for the 2007/2008 Northwest Florida Water...

  20. Lower Florida Keys National Wildlife Refuges: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Lower Florida Keys NWRs for the next 15 years. This plan outlines the refuges' vision...

  1. 2008 Florida Division of Emergency Management Lidar: Middle Suwannee River

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — LiDAR Survey for the Suwannee River Water Management District (SRWMD), Florida. The LiDAR aerial acquisition was conducted in January of 2008, and the breaklines and...

  2. Seagrass from Unified Florida Reef Tract Map (NODC Accession 0123059)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is a subset of the Unified Map representing Seagrass areas. Version 1.1 - December 2013. The Unified Florida Reef Tract Map (Unified Reef Map) provides...

  3. South Florida Seagrass Fish and Invertebrate Assessment Network (FIAN)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The South Florida Fish and Invertebrate Assessment Network (FIAN) is a monitoring project within the Comprehensive Everglades Restoration Plan (CERP). It is an...

  4. Florida Panther National Wildlife Refuge: Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) was written to guide management on Florida Panther NWR for the next 15 years. This plan outlines the Refuge vision and...

  5. Florida Investigates 2nd Possible Local Transmission of Zika Virus

    Science.gov (United States)

    ... html Florida Investigates 2nd Possible Local Transmission of Zika Virus If confirmed, cases would be first instances of ... Broward County, north of Miami. Infection with the Zika virus, which in most cases is transmitted by mosquitoes, ...

  6. Directory of the Florida Motion Picture and Television Industry.

    Science.gov (United States)

    Florida State Dept. of Commerce, Tallahassee. Div. of Economic Development.

    Designed to assist the motion picture or television producer, this directory lists organizations (producers, distributors, talent agencies, laboratories, etc.) by geographical section in Florida. Each entry includes the company address, telephone, services available, a contact person, and credits. (DAG)

  7. Initial Morphologic Evolution of Perdido Key Berm Nourishment, Florida

    Science.gov (United States)

    2013-08-01

    Nourishment , Florida by Ping Wang, Katherine E. Brutsche, Tanya M. Beck, Julie D. Rosati, and Linda S. Lillycrop PURPOSE: This Coastal and...portion of Perdido Key, including the present study area, was nourished in 1985 and 1989 (Dean et al. 1995). The 1985 beach nourishment was...Key berm nourishment , Florida. Coastal and Hydraulics Engineering Technical Note ERDC/CHL CHETN-IV-89. Vicksburg, MS: US Army Engineer Research and

  8. America's First Eco-Sustainable City Destiny, Florida

    Science.gov (United States)

    Gatewood, Roz

    2009-07-01

    Destiny, Florida is a new large-scale eco-community based on the principles of sustainability to be located in the heart of Central Florida; a project of scale and scope and a clean slate that affords for a true paradigm shift to take place on how we develop sustainable communities for the future. The project involves 41,300 acres or 64 square miles.

  9. Change detection in the Florida Bay using remote sensing

    Science.gov (United States)

    Messina, Joseph P.; Busch, Terrence V.

    1997-09-01

    The Florida Bay region is experiencing an economically and environmentally debilitating algal bloom. Remotely sensed data collected by the SPOT satellites provides fine spatial resolution data, necessary for this environment, currently available covering the spectral signature of chlorophyll. The study used SPOT multispectral data to test the utility of the green band (.5 - .6 microns) in algae detection while providing a change detection analysis of the Florida Bay for the years 1987, 1991, 1994 and 1996.

  10. Large-scale predation by river otters (Lontra canadensis) on Florida cooter (Pseudemys floridana) and Florida softshell turtles (Apalone ferox).

    Science.gov (United States)

    Stacy, Brian A; Wolf, Dan A; Wellehan, James F X

    2014-10-01

    Abstract We observed predation by river otters (Lontra canadensis) on large numbers of Florida cooter (Pseudemys floridana) and Florida softshell turtles (Apalone ferox) in two small lakes in North Central Florida, USA during a period of unusually low water levels. Carcasses were strewn on the shoreline and accumulated around floating boat docks, where some residents observed turtles being killed. We found 76 carcasses, including predominantly skeletons, and two live, severely injured turtles from one lake; however, numerous remains undoubtedly were unrecovered. The otters frequently eviscerated the turtles and removed the head and one or more appendages, including the phallus of mature males. In skeletal remains, injuries inflicted by otters were nonspecific, indistinguishable from damage caused by scavengers, or easily missed in incomplete carcasses. This report of large-scale mortality of freshwater turtles in Florida suggests that otters could have a significant impact on local turtle populations.

  11. Continuous bottom temperature measurements in strategic areas of the Florida Reef Tract at Cape Florida, 1996 - 2005 (NODC Accession 0002788)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The purpose of this project is to document bottom seawater temperature in strategic areas of the Florida Reef Tract on a continuing basis and make that information...

  12. Continuous bottom temperature measurements in strategic areas of the Florida Reef Tract at Cape Florida, 1996 - 2005 (NODC Accession 0002788)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This ongoing project began in 1988. A total of 38 subsurface recording thermographs have been deployed in the Florida Keys National Marine Sanctuary (FKNMS)and at...

  13. 2007 Florida Division of Emergency Management (FDEM) Lidar: Herbert Hoover Dike Project Area (Southeastern Florida, Lake Okeechobee Surrounding Area)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — LiDAR data was collected by Merrick & Company from September through December of 2007 for the Florida Division of Emergency Management (FDEM). The project area...

  14. 2007 - 2008 Florida Division of Emergency Management (FDEM) Lidar Project: Blocks 1 - 10 (Southeast Florida and Keys)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a topographic survey conducted for the State of Florida Division of Emergency Management LiDAR Project. These...

  15. Volume transport data from a submarine cable in the Florida Strait from 1982 to 1998 (NODC Accession 0087879)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Daily mean volume transport data of the Florida Current collected with a submarine cable spanning from South Florida to the Grand Bahama Island in the Florida Strait...

  16. Hydrology of Hunters Lake, Hernando County, Florida

    Science.gov (United States)

    Henderson, S.E.

    1986-01-01

    The size and shape of Hunters Lake, Florida has been significantly altered by development of the surrounding Spring Hill residential community. The lake is the largest in Hernando County, enlarged by lakeshore excavation and connection to nearby ponds to an area of 360 acres at an average stage of 17.2 ft above sea level. Hunters Lake is naturally a closed lake, but development of Spring Hill has resulted in a surface water outflow from the lake in its southwest corner. Inflow to the lake could occur on the east side during extreme high-water periods. The karst terrain of the Hunters Lake area is internally drained through permeable soils, depressions, and sinkholes, and natural surface drainage is absent. The underlying Floridan aquifer system is unconfined except locally near coastal springs. Flow in the groundwater system is to the west regionally and to the southwest in the immediate area of Hunters Lake. Water level gradients in the groundwater system increase from 1.4 ft/mi east of the lake to about 8 ft/mi southwest of the lake. Hunters Lake is hydraulically connected to the groundwater system, receiving groundwater on the northeast side and losing water to the groundwater system on the southwest side. This close relationship with the groundwater system is demonstrated by graphical and numerical comparison of Hunters Lake stage with water levels in nearby groundwater sites. During 1965-84, the stage of Hunters Lake fluctuated between 12.48 and 20.7 ft above sea level. Because area lakes are all directly affected by groundwater levels, they also show a close relationship with water levels in Hunters Lake. Analysis of water quality data for Hunters Lake indicates that the water of the lake is a soft calcium bicarbonate type with ionic concentrations higher than in water from nearby shallow wells and lower than in water from the Upper Floridan aquifer. Samples collected in 1981-1983 indicate slightly higher levels of ionic concentration than in 1965

  17. The synapsin gene family in basal chordates: evolutionary perspectives in metazoans

    Directory of Open Access Journals (Sweden)

    De Bernardi Fiorenza

    2010-01-01

    Full Text Available Abstract Background Synapsins are neuronal phosphoproteins involved in several functions correlated with both neurotransmitter release and synaptogenesis. The comprehension of the basal role of the synapsin family is hampered in vertebrates by the existence of multiple synapsin genes. Therefore, studying homologous genes in basal chordates, devoid of genome duplication, could help to achieve a better understanding of the complex functions of these proteins. Results In this study we report the cloning and characterization of the Ciona intestinalis and amphioxus Branchiostoma floridae synapsin transcripts and the definition of their gene structure using available C. intestinalis and B. floridae genomic sequences. We demonstrate the occurrence, in both model organisms, of a single member of the synapsin gene family. Full-length synapsin genes were identified in the recently sequenced genomes of phylogenetically diverse metazoans. Comparative genome analysis reveals extensive conservation of the SYN locus in several metazoans. Moreover, developmental expression studies underline that synapsin is a neuronal-specific marker in basal chordates and is expressed in several cell types of PNS and in many, if not all, CNS neurons. Conclusion Our study demonstrates that synapsin genes are metazoan genes present in a single copy per genome, except for vertebrates. Moreover, we hypothesize that, during the evolution of synapsin proteins, new domains are added at different stages probably to cope up with the increased complexity in the nervous system organization. Finally, we demonstrate that protochordate synapsin is restricted to the post-mitotic phase of CNS development and thereby is a good marker of postmitotic neurons.

  18. Food Waste Auditing at Three Florida Schools

    Directory of Open Access Journals (Sweden)

    Ann C. Wilkie

    2015-01-01

    Full Text Available School cafeterias are a significant source of food waste and represent an ideal opportunity for diverting food waste from landfills. In this study, cafeteria waste audits were conducted at three Florida schools. Food waste comprised the largest fraction of school cafeteria waste streams, ranging from 47% to 58%, followed by milk, paper products (tissue, milk cartons, pasteboard, paper plates, and cardboard, and plastics (plastic wrap, packaging, and utensils. Metal and glass comprised the smallest fraction of the waste stream. Average total waste generation ranged from 50.5 to 137.6 g·student−1·day−1. The mean generation rates for food waste ranged from 24.7 to 64.9 g·student−1·day−1. The overall average for cafeteria waste generation among all three schools was 102.3 g·student−1·day−1, with food waste alone contributing 52.2 g·student−1·day−1. There are two primary approaches to diverting school food waste from landfills: reduction and recycling. Food waste can be reduced through educating students and staff in order to change behaviors that cause food waste. Food waste can be collected and recycled through composting or anaerobic digestion in order to generate beneficial end products, including soil amendments and bioenergy. Over 75% of the cafeteria waste measured in this study could be recycled in this manner.

  19. 2005/2006 Southwest Florida Water Management District (SWFWMD) Lidar: Peace River South (including Carter Creek)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Light Detection and Ranging (LiDAR) LAS dataset is a survey of select areas within Southwest Florida. These data were produced for the Southwest Florida Water...

  20. Volume transport data from a submarine cable in the Florida Strait in 2013 (NODC Accession 0115895)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Daily mean and raw voltage volume transport data of the Florida Current collected with a submarine cable spanning from South Florida to the Grand Bahama Island in...

  1. Volume transport data from a submarine cable in the Florida Strait from 2015 (NCEI Accession 0140278)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Daily mean and raw voltage volume transport data of the Florida Current collected with a submarine cable spanning from South Florida to the Grand Bahama Island in...

  2. Volume transport data from a submarine cable in the Florida Strait in 2014 (NODC Accession 0125429)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Daily mean and raw voltage volume transport data of the Florida Current collected with a submarine cable spanning from South Florida to the Grand Bahama Island in...

  3. IKONOS Imagery for southern Florida used to map shallow-water seafloor habitats

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This project is a cooperative effort between the Florida Fish and Wildlife Conservation Commission, Florida Department of Environmental Protection, National Park...

  4. Hurricane Wilma Aerial Photography: High-Resolution Imagery of the Florida Coast After Landfall

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The imagery posted on this site is of the Florida coast after Hurricane Wilma made landfall. The regions photographed range from Key West to Sixmile Bend, Florida....

  5. Florida Men Sentenced for Poisoning Wildlife and Hunting Dogs in Bullock County

    Science.gov (United States)

    ATLANTA - Daryl Fischer of Seminole, Florida and Russell Taylor of Loxahatchee, Florida were sentenced on Tuesday, April 28, 2015, to terms of probation for improper use of the pesticide Aldicarb, which is marketed as Temik, announced the United Sta

  6. Scleria lacustris (Cyperaceae), an aquatic and wetland sedge introduced to Florida

    Science.gov (United States)

    Jacono, C.C.

    2001-01-01

    A non-native species of Scleria, S. lacustris is reported from six counties and three major hydrologic regions in Florida. Biogeography and habitat in Florida are addressed. A description, key features and illustration are presented.

  7. Cost-Effectiveness Analysis of the Residential Provisions of the 2015 IECC for Florida

    Energy Technology Data Exchange (ETDEWEB)

    Mendon, Vrushali V. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Zhao, Mingjie [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Taylor, Zachary T. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Poehlman, Eric A. [Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

    2016-02-15

    The 2015 IECC provides cost-effective savings for residential buildings in Florida. Moving to the 2015 IECC from the 2012 IECC base code is cost-effective for residential buildings in all climate zones in Florida.

  8. 2008 Northwest Florida Water Management District (NWFWMD) LiDAR: Inland Okaloosa County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a survey of inland Okaloosa County, Florida not covered in the 2008 Florida Department of Emergency...

  9. 2007 Florida Division of Emergency Management (FDEM) Lidar Project: Wakulla County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This report pertains to a Specific Purpose Lidar Survey of Wakulla County, Florida, conducted in the summer of 2007 for the Florida Division of Emergency Management...

  10. Shifting Diagnostic Systems for Defining Intellectual Disability in Death Penalty Cases: Hall vs. Florida

    Science.gov (United States)

    Mukherjee, Mina; Westphal, Alexander

    2015-01-01

    The case of Hall vs. Florida tested Florida's so called "bright line rule" in determining intellectual disability in capital cases. The Supreme Court Decision reflects a more general trend from categorical to dimensional approaches in psychiatric diagnostic systems.

  11. 2005 Southwest Florida Water Management District (SWFWMD) Lidar: Little Manatee District

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Light Detection and Ranging (LiDAR) LAS dataset is a survey of select areas within Southwest Florida. These data were produced for the Southwest Florida Water...

  12. U.S. Geological Survey Program on the South Florida Ecosystem - Proceedings of South Florida Restoration Science Forum, May 17-19, 1999, Boca Raton, Florida

    Science.gov (United States)

    1999-01-01

    Protection Agency and the U.S. Army Corps of Engineers. 1The Institute for Bird Populations, P.O. Box 1346, Point Reyes Station, California 94956-1346...efforts have been focused on Whitewater Bay/ Ponce de Leon Bay and Johnson Key Basin in western Florida Bay. Sampling continues on both the Tortugas and

  13. U.S. Geological Survey Program on the South Florida Ecosystem; proceedings of South Florida Restoration Science Forum, May 17-19, 1999, Boca Raton, Florida

    Science.gov (United States)

    Gerould, Sarah; Higer, Aaron

    1999-01-01

    The purpose of the forum is to highlight the powerful connection between science and management decisions in restoration efforts. The public's investment in science is paying off in support of better management decisions and restoration of imperiled south Florida Ecosystems, including the internationally recognized, globally significant Everglades. The forum affords a unique opportunity for elected officials and other policy- and decision makers, along with the general public, to see--under one roof--highlights of the most significant restoration science and management efforts underway. The forum promotes the link between science and management. Scientists and decisionmakers will come together to discuss the needs of each in order to ensure that plans for restoration are based in science and are the most cost effective and highest quality possible. Continued vigilance over south Florida ecosystems is essential to prevent further harm and to restore them. Representatives from numerous federal, state, local, and nongovernmental entities are organizing the forum for the Science Coordination Team of the South Florida Ecosystem Working Group. The U.S. Geological Survey and the South Florida Water Management District are the primary hosts of the forum.

  14. A habitat assessment for Florida panther population expansion into central Florida

    Science.gov (United States)

    Thatcher, C.A.; Van Manen, F.T.; Clark, J.D.

    2009-01-01

    One of the goals of the Florida panther (Puma concolor coryi) recovery plan is to expand panther range north of the Caloosahatchee River in central Florida. Our objective was to evaluate the potential of that region to support panthers. We used a geographic information system and the Mahalanobis distance statistic to develop a habitat model based on landscape characteristics associated with panther home ranges. We used cross-validation and an independent telemetry data set to test the habitat model. We also conducted a least-cost path analysis to identify potential habitat linkages and to provide a relative measure of connectivity among habitat patches. Variables in our model were paved road density, major highways, human population density, percentage of the area permanently or semipermanently flooded, and percentage of the area in natural land cover. Our model clearly identified habitat typical of that found within panther home ranges based on model testing with recent telemetry data. We identified 4 potential translocation sites that may support a total of approximately 36 panthers. Although we identified potential habitat linkages, our least-cost path analyses highlighted the extreme isolation of panther habitat in portions of the study area. Human intervention will likely be required if the goal is to establish female panthers north of the Caloosahatchee in the near term.

  15. Genetic Introgression and the Survival of Florida Panther Kittens.

    Science.gov (United States)

    Hostetler, Jeffrey A; Onorato, David P; Nichols, James D; Johnson, Warren E; Roelke, Melody E; O'Brien, Stephen J; Jansen, Deborah; Oli, Madan K

    2010-11-01

    Estimates of survival for the young of a species are critical for population models. These models can often be improved by determining the effects of management actions and population abundance on this demographic parameter. We used multiple sources of data collected during 1982-2008 and a live recapture-dead recovery modeling framework to estimate and model survival of Florida panther (Puma concolor coryi) kittens (age 0 - 1 year). Overall, annual survival of Florida panther kittens was 0.323 ± 0.071 (SE), which was lower than estimates used in previous population models. In 1995, female pumas from Texas (P. c. stanleyana) were released into occupied panther range as part of an intentional introgression program to restore genetic variability. We found that kitten survival generally increased with degree of admixture: F(1) admixed and backcrossed to Texas kittens survived better than canonical Florida panther and backcrossed to canonical kittens. Average heterozygosity positively influenced kitten and older panther survival, whereas index of panther abundance negatively influenced kitten survival. Our results provide strong evidence for the positive population-level impact of genetic introgression on Florida panthers. Our approach to integrate data from multiple sources was effective at improving robustness as well as precision of estimates of Florida panther kitten survival, and can be useful in estimating vital rates for other elusive species with sparse data.

  16. Genetic introgression and the survival of Florida panther kittens

    Science.gov (United States)

    Hostetler, Jeffrey A.; Onorato, David P.; Nichols, James D.; Johnson, Warren E.; Roelke, Melody E.; O'Brien, Stephen J.; Jansen, Deborah; Oli, Madan K.

    2010-01-01

    Estimates of survival for the young of a species are critical for population models. These models can often be improved by determining the effects of management actions and population abundance on this demographic parameter. We used multiple sources of data collected during 1982–2008 and a live-recapture dead-recovery modeling framework to estimate and model survival of Florida panther (Puma concolor coryi) kittens (age 0–1 year). Overall, annual survival of Florida panther kittens was 0.323 ± 0.071 (SE), which was lower than estimates used in previous population models. In 1995, female pumas from Texas (P. c. stanleyana) were released into occupied panther range as part of an intentional introgression program to restore genetic variability. We found that kitten survival generally increased with degree of admixture: F1 admixed and backcrossed to Texas kittens survived better than canonical Florida panther and backcrossed to canonical kittens. Average heterozygosity positively influenced kitten and older panther survival, whereas index of panther abundance negatively influenced kitten survival. Our results provide strong evidence for the positive population-level impact of genetic introgression on Florida panthers. Our approach to integrate data from multiple sources was effective at improving robustness as well as precision of estimates of Florida panther kitten survival, and can be useful in estimating vital rates for other elusive species with sparse data.

  17. Tidal Motion in a Complex Inlet and Bay System, Ponce de Leon Inlet, Florida

    Science.gov (United States)

    2000-01-01

    REPORT TYPE N/A 3. DATES COVERED - 4. TITLE AND SUBTITLE Tidal Motion in a Complex Inlet and Bay System, Ponce de Leon Inlet, Florida 5a...investigated in Ponce de Leon (Ponce) Inlet, Florida, and its bay channels through a 10-week data-collection campaign and two-dimensional numerical...Beach, Florida Summer 2000 Tidal Motion in a Complex Inlet and Bay System, Ponce de Leon Inlet, Florida Adele Militellot and Gary A. Zarillo:j: t

  18. 77 FR 64336 - Public Water System Supervision Program Revision for the State of Florida

    Science.gov (United States)

    2012-10-19

    ... AGENCY Public Water System Supervision Program Revision for the State of Florida AGENCY: Environmental... of Florida is revising its Public Water System Supervision Program by adopting the Lead and Copper... Florida's Public Water System Supervision Program. DATES: Any interested person may request a...

  19. 40 CFR 81.95 - Central Florida Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Quality Control Regions § 81.95 Central Florida Intrastate Air Quality Control Region. The Central Florida Intrastate Air Quality Control Region consists of the territorial area encompassed by the boundaries of the... 40 Protection of Environment 17 2010-07-01 2010-07-01 false Central Florida Intrastate Air...

  20. Florida Public Health Training Center: Evidence-Based Online Mentor Program

    Science.gov (United States)

    Frahm, Kathryn A.; Alsac-Seitz, Biray; Mescia, Nadine; Brown, Lisa M.; Hyer, Kathy; Liburd, Desiree; Rogoff, David P.; Troutman, Adewale

    2013-01-01

    This article describes an Online Mentor Program (OMP) designed to support and facilitate mentorships among and between Florida Department of Health (FDOH) employees and USF College of Public Health students using a Web-based portal. The Florida Public Health Training Center (FPHTC) at the University of South Florida (USF) College of Public Health…

  1. Final Environmental Assessment for Long-Term Vegetation Control for Eglin Air Force Base, Florida

    Science.gov (United States)

    2008-07-14

    Milkweed Asclepias humistrata Florida Black Bear Ursus americanus floridanus Pitcherplant Sarracenia spp. Gray Fox Urocyon cinereoargenteus Affected...Florida Black Bear LT* – Plants Andropogon arctatus Pine-Woods Bluestem LT – Asclepias viridula Southern Milkweed LT – Baptisia calycosa var villosa... Milkweed Asclepias humistrata Florida Black Bear Ursus americanus floridanus Pitcherplant Sarracenia spp. Gray Fox Urocyon cinereoargenteus Wetland

  2. 7 CFR 915.306 - Florida avocado grade, pack, and container marking regulation.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 8 2010-01-01 2010-01-01 false Florida avocado grade, pack, and container marking... AGRICULTURE AVOCADOS GROWN IN SOUTH FLORIDA Container and Pack Regulations § 915.306 Florida avocado grade, pack, and container marking regulation. (a) No handler shall handle any variety of avocados grown...

  3. 78 FR 30782 - Avocados Grown in South Florida; Change in Minimum Grade Requirements

    Science.gov (United States)

    2013-05-23

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 915 Avocados Grown in South Florida... prescribed under the Florida avocado marketing order (order). The order regulates the handling of avocados grown in South Florida, and is administered locally by the Avocado Administrative Committee...

  4. 40 CFR 81.96 - West Central Florida Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 17 2010-07-01 2010-07-01 false West Central Florida Intrastate Air... Air Quality Control Regions § 81.96 West Central Florida Intrastate Air Quality Control Region. The West Central Florida Intrastate Air Quality Control Region consists of the territorial area...

  5. 75 FR 52967 - Final Environmental Impact Statement and South Florida and Caribbean Parks Exotic Plant...

    Science.gov (United States)

    2010-08-30

    ... Palm Beach, Florida. A Notice of Intent to prepare an environmental impact statement (EIS) for South... National Park Service Final Environmental Impact Statement and South Florida and Caribbean Parks Exotic... environmental impact statement for the South Florida and Caribbean Parks Exotic Plant Management Plan....

  6. NASA Applied Sciences' DEVELOP National Program: Summer 2010 Florida Agriculture

    Science.gov (United States)

    Cooley, Zachary C.; Billiot, Amanda; Lee, Lucas; McKee, Jake

    2010-01-01

    The main agricultural areas in South Florida are located within the fertile land surrounding Lake Okeechobee. The Atlantic Watershed monthly rainfall anomalies showed a weak but statistically significant correlation to the Oceanic Nino Index (ONI). No other watershed s anomalies showed significant correlations with ONI or the Southern Oscillation Index (SOI). During La Nina months, less sea breeze days and more disturbed days were found to occur compared to El Nino and neutral months. The increase in disturbed days can likely by attributed to the synoptic pattern during La Nina, which is known to be favorable for tropical systems to follow paths that affect South Florida. Overall, neither sea breeze rainfall patterns nor total rainfall patterns in South Florida s main agricultural areas were found to be strongly influenced by the El Nino Southern Oscillation during our study time.

  7. The South Florida Environment: A Region Under Stress

    Science.gov (United States)

    McPherson, Benjamin F.; Halley, Robert B.

    1996-01-01

    This report provides an overview of the environmental setting in South Florida and serves as review and framework for developing U.S. Geological Survey programs in the region. The report describes the predevelopment and the current (present-day) environmental conditions in South Florida with emphasis on the quantity and quality of water. The geographical area covered is the southern one-half of the State and includes the South Florida National Water-Quality Assessment study area and adjacent coastal waters. This study area covers about 19,500 square miles and is the watershed of the larger regional ecosystem. The regional ecosystem includes the coastal waters between Charlotte Harbor on the Gulf of Mexico and the St. Lucie River on the Atlantic Ocean and the lands that drain into these waters.

  8. Holocene Infilling History of Tampa Bay, Florida

    Science.gov (United States)

    Brooks, G. R.; Larson, R. A.; Cronin, T.; Willard, D.

    2007-05-01

    Tampa Bay is a shallow, sediment-starved estuary located along the central Florida Gulf coast. Based upon sedimentologic, biostratigraphic, and geochronologic analyses of 120 sediment cores and 190 surface sediment samples, karst-controlled basins located in the mid to upper estuary were found to contain a continuous sedimentary record documenting the Holocene sea-level rise and infilling history. The basal unit sampled in cores consists of organic-rich and/or carbonate-rich sediments containing freshwater fauna. Interpreted as lake deposits, the surficial sediments of these units were dated at approximately 8-9 ka suggesting that isolated sinkhole lakes occupied the region prior to being flooded by the Holocene sea-level rise. Overlying the lake deposits, dm-scale, organic-rich muds containing brackish water fauna, represent the transition from fresh to marine conditions as sea level flooded the region. The flooding surface itself is generally undefined, but sometimes represented by a mm-scale layer of shell fragments likely representing a lag deposit. Age dates bracketing this layer show that flooding occurred approximately 6-7 ka. Overlying sediments consist of 3-4 m of organic-rich, sandy muds with typical estuarine fauna. Age dates from the base of this unit indicate estuarine conditions became established approximately 5.5-6 ka. The modern expression of karst basins is a series of shallow, bathymetric depressions, likely reflecting the historically slow rate (0.030-0.065 cm/yr) of fine- grained sediment accumulation. These shallow depressions continue to function as fine-grained sediment sinks, but are now rapidly filling as the rate of accumulation has dramatically increased by approximately one order-of-magnitude (0.16-0.32 cm/yr) within the past 100 years, likely due to human activities.

  9. Winter habitat preferences for Florida manatees and vulnerability to cold.

    Directory of Open Access Journals (Sweden)

    David W Laist

    Full Text Available To survive cold winter periods most, if not all, Florida manatees rely on warm-water refuges in the southern two-thirds of the Florida peninsula. Most refuges are either warm-water discharges from power plant and natural springs, or passive thermal basins that temporarily trap relatively warm water for a week or more. Strong fidelity to one or more refuges has created four relatively discrete Florida manatee subpopulations. Using statewide winter counts of manatees from 1999 to 2011, we provide the first attempt to quantify the proportion of animals using the three principal refuge types (power plants, springs, and passive thermal basins statewide and for each subpopulation. Statewide across all years, 48.5% of all manatees were counted at power plant outfalls, 17.5% at natural springs, and 34.9 % at passive thermal basins or sites with no known warm-water features. Atlantic Coast and Southwest Florida subpopulations comprised 82.2% of all manatees counted (45.6% and 36.6%, respectively with each subpopulation relying principally on power plants (66.6% and 47.4%, respectively. The upper St. Johns River and Northwest Florida subpopulations comprised 17.8% of all manatees counted with almost all animals relying entirely on springs (99.2% and 88.6% of those subpopulations, respectively. A record high count of 5,076 manatees in January 2010 revealed minimum sizes for the four subpopulations of: 230 manatees in the upper St. Johns River; 2,548 on the Atlantic Coast; 645 in Northwest Florida; and 1,774 in Southwest Florida. Based on a comparison of carcass recovery locations for 713 manatees killed by cold stress between 1999 and 2011 and the distribution of known refuges, it appears that springs offer manatees the best protection against cold stress. Long-term survival of Florida manatees will require improved efforts to enhance and protect manatee access to and use of warm-water springs as power plant outfalls are shut down.

  10. Winter habitat preferences for Florida manatees and vulnerability to cold.

    Science.gov (United States)

    Laist, David W; Taylor, Cynthia; Reynolds, John E

    2013-01-01

    To survive cold winter periods most, if not all, Florida manatees rely on warm-water refuges in the southern two-thirds of the Florida peninsula. Most refuges are either warm-water discharges from power plant and natural springs, or passive thermal basins that temporarily trap relatively warm water for a week or more. Strong fidelity to one or more refuges has created four relatively discrete Florida manatee subpopulations. Using statewide winter counts of manatees from 1999 to 2011, we provide the first attempt to quantify the proportion of animals using the three principal refuge types (power plants, springs, and passive thermal basins) statewide and for each subpopulation. Statewide across all years, 48.5% of all manatees were counted at power plant outfalls, 17.5% at natural springs, and 34.9 % at passive thermal basins or sites with no known warm-water features. Atlantic Coast and Southwest Florida subpopulations comprised 82.2% of all manatees counted (45.6% and 36.6%, respectively) with each subpopulation relying principally on power plants (66.6% and 47.4%, respectively). The upper St. Johns River and Northwest Florida subpopulations comprised 17.8% of all manatees counted with almost all animals relying entirely on springs (99.2% and 88.6% of those subpopulations, respectively). A record high count of 5,076 manatees in January 2010 revealed minimum sizes for the four subpopulations of: 230 manatees in the upper St. Johns River; 2,548 on the Atlantic Coast; 645 in Northwest Florida; and 1,774 in Southwest Florida. Based on a comparison of carcass recovery locations for 713 manatees killed by cold stress between 1999 and 2011 and the distribution of known refuges, it appears that springs offer manatees the best protection against cold stress. Long-term survival of Florida manatees will require improved efforts to enhance and protect manatee access to and use of warm-water springs as power plant outfalls are shut down.

  11. Assessment of acreage and vegetation change in Florida`s Big Bend tidal wetlands using satellite imagery

    Energy Technology Data Exchange (ETDEWEB)

    Raabe, E.A.; Stumpf, R.P. [Geological Survey, St. Petersburg, FL (United States)

    1997-06-01

    Fluctuations in sea level and impending development on the west coast of Florida have aroused concern for the relatively pristine tidal marshes of the Big Bend. Landsat Thematic Mapper (TM) images for 1986 and 1995 are processed and evaluated for signs of change. The images cover 250 km of Florida`s Big Bend Gulf Coast, encompassing 160,000 acres of tidal marshes. Change is detected using the normalized difference vegetation index (NDVI) and land cover classification. The imagery shows negligible net loss or gain in the marsh over the 9-year period. However, regional changes in biomass are apparent and are due to natural disturbances such as low winter temperatures, fire, storm surge, and the conversion of forest to marsh. Within the marsh, the most prominent changes in NDVI and in land cover result from the recovery of mangroves from freezes, a decline of transitional upland vegetation, and susceptibility of the marsh edge and interior to variations in tidal flooding.

  12. 76 FR 65378 - Safety Zone; The Florida Orchestra Pops in the Park Fireworks Display, Tampa Bay, St. Petersburg, FL

    Science.gov (United States)

    2011-10-21

    ... SECURITY Coast Guard 33 CFR Part 165 RIN 1625-AA00 Safety Zone; The Florida Orchestra Pops in the Park... vicinity of Spa Beach in St. Petersburg, Florida during The Florida Orchestra Pops in the Park Fireworks... Saturday, October 22, 2011, The Florida Orchestra Pops in the Park Fireworks Display is scheduled to...

  13. Marketing Fresh Produce to Local Schools: The North Florida Cooperative Experience [and] Cultivating Schools as Customers in a Local Market: The New North Florida Cooperative Experience [and] Acquiring Capital and Establishing a Credit History: The North Florida Cooperative Experience [and] Success of the New North Florida Cooperative: A Progress Report on Producer Direct Sales to School Districts. Small Farmer Success Story. Bulletins 1-4.

    Science.gov (United States)

    Department of Agriculture, Washington, DC.

    These four bulletins describe how a group of limited-resource small farmers in northern Florida's Jackson County, the USDA, the West Florida Resource Conservation and Development Council, Florida A&M University, and the Federation of Southern Cooperatives organized the New North Florida Cooperative to increase farm income by introducing…

  14. Phased Retrofits in Existing Homes in Florida Phase II. Shallow Plus Retrofits

    Energy Technology Data Exchange (ETDEWEB)

    Sutherland, K. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Parker, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Martin, E. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Chasar, D. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States); Amos, B. [Building America Partnership for Improved Residential Construction, Cocoa, FL (United States)

    2016-02-01

    The BAPIRC team and Florida Power and Light (FPL) electric utility pursued a pilot phased energy-efficiency retrofit program in Florida by creating detailed data on the energy and economic performance of two levels of retrofit - simple and deep. For this Phased Deep Retrofit (PDR) project, a total of 56 homes spread across the utility partner's territory in east central Florida, southeast Florida, and southwest Florida were instrumented between August 2012 and January 2013, and received simple pass-through retrofit measures during the period of March 2013 - June 2013. Ten of these homes received a deeper package of retrofits during August 2013 - December 2013.

  15. Effects of the Gulf Oil Spill in Escambia County, Florida

    Science.gov (United States)

    Killingsworth, Kelcey Ray

    2012-01-01

    The purpose of this study was to assess the impact of the British Petroleum Gulf Oil Spill on resource change, psychological stress, and resilience for business owners, residents, and workers in Escambia County, Florida. This study was based on Hobfoll's (1988, 1989) Conservation of Resources theory. All business owners, residents, and…

  16. Florida's Public Education Spending. School Choice Issues in the State

    Science.gov (United States)

    Aud, Susan

    2006-01-01

    This study analyzes and explains Florida's education finance system. It explains the sources of revenue and the expenditure of funds, reporting figures for each of the state's 67 districts. It also analyzes the trend in current expenditures --that is, the day-to-day operating costs of schools--to address the question of whether they have been…

  17. The Fact Book: Report for the Florida College System, 2015

    Science.gov (United States)

    Florida Department of Education, 2015

    2015-01-01

    This 2015 fact book for the Florida College System is divided into the following categories: (1) Student Information, which includes fall, annual, FTE, and program enrollment statistics, as well as credit program completion statistics; (2) Employee Information, which includes statistics regarding employee headcount by occupational activity, and…

  18. The Fact Book: Report for the Florida College System, 2014

    Science.gov (United States)

    Florida Department of Education, 2014

    2014-01-01

    This 2014 fact book for the Florida College System is divided into the following categories: (1) Student Information, which includes fall, annual, FTE, and program enrollment statistics, as well as credit program completion statistics; (2) Employee Information, which includes statistics regarding employee headcount by occupational activity, and…

  19. Making Online Professional Development Work for Florida Project Learning Tree

    Science.gov (United States)

    McConnell, Lindsey C.; Monroe, Martha C.

    2012-01-01

    Large distances and small budgets had made it challenging for Florida Project Learning Tree (PLT) to conduct in-person workshops with volunteer 4-H leaders to disseminate the PreK-8 guide. An online version of a PLT workshop was developed to overcome these barriers. Formative evaluation data suggested that the program effectively introduced PLT to…

  20. Hurricanes, submarine groundwater discharge, and Florida's red tides

    Science.gov (United States)

    Hu, Chuanmin; Muller-Karger, Frank E.; Swarzenski, Peter W.

    2006-06-01

    A Karenia brevis Harmful Algal Bloom affected coastal waters shallower than 50 m off west-central Florida from January 2005 through January 2006, showing a sustained anomaly of ~1 mg chlorophyll m-3 over an area of up to 67,500 km2. Red tides occur in the same area (approximately 26-29°N, 82-83°W) almost every year, but the intense 2005 bloom led to a widespread hypoxic zone (dissolved oxygen marine mammals. Runoff alone provided insufficient nitrogen to support this bloom. We pose the hypothesis that submarine groundwater discharge (SGD) provides the missing nutrients, and indeed can trigger and support the recurrent red tides off west-central Florida. SGD inputs of dissolved inorganic nitrogen (DIN) in Tampa Bay alone are ~35% of that discharged by all central Florida rivers draining west combined. We propose that the unusual number of hurricanes in 2004 resulted in high runoff, and in higher than normal SGD emerging along the west Florida coast throughout 2005, initiating and fueling the persistent HAB. This mechanism may also explain recurrent red tides in other coastal regions of the Gulf of Mexico.

  1. School Choice: The Fiscal Impact of Home Education in Florida

    Science.gov (United States)

    Sutton, Lenford C.; Bogan, Yolanda K. H.

    2005-01-01

    This article provides a description of home-based education in the state of Florida and addresses the financial queries that often arise in the debate regarding the probative value of homeschooling and its effects on the financial coffers of state legislatures. This simple analysis of home-based education and its financial implications for Florida…

  2. Environmental Contaminants Evaluation of St. Joseph Bay, Florida

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The U.S. Fish and Wildlife Service (Service) conducted field work in St. Joseph Bay, Florida, for 2-week periods during each of the summers of 1991, 1992, and 1993....

  3. Architecture of the Florida power grid as a complex network

    Science.gov (United States)

    Xu, Yan; Gurfinkel, Aleks Jacob; Rikvold, Per Arne

    2014-05-01

    We study the Florida high-voltage power grid as a technological network embedded in space. Measurements of geographical lengths of transmission lines, the mixing of generators and loads, the weighted clustering coefficient, as well as the organization of edge conductance weights show a complex architecture quite different from random-graph models usually considered. In particular, we introduce a parametrized mixing matrix to characterize the mixing pattern of generators and loads in the Florida Grid, which is intermediate between the random mixing case and the semi-bipartite case where generator-generator transmission lines are forbidden. Our observations motivate an investigation of optimization (design) principles leading to the structural organization of power grids. We thus propose two network optimization models for the Florida Grid as a case study. Our results show that the Florida Grid is optimized not only by reducing the construction cost (measured by the total length of power lines), but also through reducing the total pairwise edge resistance in the grid, which increases the robustness of power transmission between generators and loads against random line failures. We then embed our models in spatial areas of different aspect ratios and study how this geometric factor affects the network structure, as well as the box-counting fractal dimension of the grids generated by our models.

  4. Pyrethroid resistance is widespread among Florida populations of Aedes aegypti

    Science.gov (United States)

    Aedes aegypti is an efficient vector of a number of diseases that affect man and is of increasing concern because of the reemergence of dengue and recent identification of locally acquired chikungunya in Florida. Pesticide resistance in this species has been demonstrated in several neighboring coun...

  5. Controversy over Biomass Plant at Florida State Heats up

    Science.gov (United States)

    Mangan, Katherine

    2009-01-01

    This article reports that Florida State University officials are gearing up for what could be another bruising battle this month over a proposed biomass plant that could bring the campus cleaner, cheaper energy and monetary support for alternative-energy research. Or, it could bring noise and pollution to a nearby neighborhood, according to…

  6. Puerto Rico and Florida manatees represent genetically distinct groups

    Science.gov (United States)

    Hunter, Margaret E.; Mignucci-Giannoni, Antonio A.; Tucker, Kimberly Pause; King, Timothy L.; Bonde, Robert K.; Gray, Brian A.; McGuire, Peter M.

    2012-01-01

    The West Indian manatee (Trichechus manatus) populations in Florida (T. m. latirostris) and Puerto Rico (T. m. manatus) are considered distinct subspecies and are listed together as endangered under the United States Endangered Species Act. Sustained management and conservation efforts for the Florida subspecies have led to the suggested reclassification of the species to a threatened or delisted status. However, the two populations are geographically distant, morphologically distinct, and habitat degradation and boat strikes continue to threaten the Puerto Rico population. Here, 15 microsatellite markers and mitochondrial control region sequences were used to determine the relatedness of the two populations and investigate the genetic diversity and phylogeographic organization of the Puerto Rico population. Highly divergent allele frequencies were identified between Florida and Puerto Rico using microsatellite (F ST = 0.16; R ST = 0.12 (P ST = 0.66; Φ ST = 0.50 (P E = 0.45; NA = 3.9), were similar, but lower than those previously identified in Florida (HE = 0.48, NA = 4.8). Within Puerto Rico, the mitochondrial genetic diversity values (π = 0.001; h = 0.49) were slightly lower than those previously reported (π = 0.002; h = 0.54) and strong phylogeographic structure was identified (F ST global = 0.82; Φ ST global = 0.78 (P manatee population.

  7. Business. Revised Summary Report: Technical Employment in Northeast Florida.

    Science.gov (United States)

    Baker, William E.; And Others

    The document is one of five summary reports, all part of a Pre-Technical Curriculum Planning Project for secondary students who aspire to technical employment or post secondary technical education. This report represents the results of an assessment of the northeast Florida areas technical occupations in business. A three-phase approach was…

  8. Statewide Dropout Prevention Database: What's Happening in Florida.

    Science.gov (United States)

    Repetto, Jeanne B.; And Others

    Project RETAIN (Retention in Education Technical Assistance and Information Network) is a Florida project that assists school districts through identification and dissemination of effective practices that keep students with mild disabilities in school. One part of the project was the development of a database of school district efforts in the area…

  9. Florida Vocational Program Guide for Basic Precision Machining, Precision Machining.

    Science.gov (United States)

    University of South Florida, Tampa. Dept. of Adult and Vocational Education.

    This program guide has been developed to provide information that will be useful to local school district and community college administrators, instructors, program advisory committee members, regional coordinating councils, and others charged with the responsibility of offering vocational education programs in Florida. It identifies the major…

  10. Dueling Philosophies: Inclusion or Separation for Florida's English Language Learners?

    Science.gov (United States)

    Platt, Elizabeth; Harper, Candace; Mendoza, Maria Beatriz

    2003-01-01

    Presents results of a survey of administrators in Florida public schools who oversee the implementation of English-as-a-Second-Language (ESL) instruction. Reviews the origins and philosophies of inclusion and separation approaches to ESL in public schools. Reports widely varying opinions concerning approaches to ESL. (Author/VWL)

  11. Release and establishment of Megamelus scutellaris (Hemiptera: Delphacidae) in Florida

    Science.gov (United States)

    Megamelus scutellaris (Berg) (Hemiptera: Delphacidae) was recently developed as a classical biological control agent for waterhyacinth, Eichhornia crassipes Mart. Solms, and released in Florida. Releases were conducted at 10 sites around the state every 4-6 weeks until late fall then halted until t...

  12. Ticks associated with domestic dogs and cats in Florida, USA

    Science.gov (United States)

    Voluntary collections of ticks from domestic dogs and cats by veterinary practitioners across Florida were conducted over a 10 month period. Of the 1,337 ticks submitted, five species of ixodid ticks were identified and included Rhipicephalus sanguineus, Amblyomma americanum, A. maculatum, Dermacen...

  13. Cable Television Franchising in Florida: An Analysis of Selected Franchises.

    Science.gov (United States)

    Mengel, Lucia M.

    Cable Television franchises in 20 Florida communities were analyzed to determine rates and citizens' rights of access as required by FCC regulations. It was found that rates ranged from $4.95 to $8.75, with the average monthly service at $5.65. Fees had little to do with an overall citizens' rights; the companies with higher rates generally made…

  14. Caribbean Fruit Fly (Diptera: Tephritidae) and Small Fruit in Florida

    Science.gov (United States)

    Tephritid fruit flies are among the most important pests of fruits and vegetables worldwide. The Caribbean fruit fly, Anastrepha suspensa (Loew), is a tephritid pest that became established in Florida following introduction in 1965. Populations of this fruit fly also occur in Puerto Rico and Cuba, ...

  15. Consumption of bird eggs by invasive Burmese Pythons in Florida

    Science.gov (United States)

    Dove, Carla J.; Reed, Robert N.; Snow, Ray W.

    2012-01-01

    Burmese Pythons (Python molurus bivittatus or P. bivittatus) have been reported to consume 25 species of adult birds in Everglades National Park, Florida (Dove et al. 2011), but until now no records documented this species eating bird eggs. Here we report three recent cases of bird-egg consumption by Burmese Pythons and discuss egg-eating in basal snakes.

  16. Healthful and nutritional components in select Florida tropical fruits

    Science.gov (United States)

    Fourteen tropical fruits from south Florida (red guava, white guava, carambola, red pitaya (red dragon), white pitaya (white dragon), mamey, sapodilla, lychee, longan, green mango, ripe mango, green papaya and ripe papaya) were evaluated for phenolic compounds, antioxidant activity, ascorbic acid (v...

  17. Education: Organic Refresher Course to Debut in Florida.

    Science.gov (United States)

    Dagani, Ron

    1980-01-01

    Announces the introduction of a refresher course designed to expose senior chemists to modern organic chemistry, delving into such topics as new synthetic methods, molecular orbital theory, heterocyclic and organometallic chemistry, and C-13 nuclear magnetic resonance spectrometry, offered at the University of Florida in Gainesville in March 1981.…

  18. Integrating Sunflower Oil Seed Crops into Florida Horticultural Production Systems

    Science.gov (United States)

    Locally produced biodiesel feedstock plant oil creates a unique possibility to integrate multiple-goal oriented cover crops into Florida horticultural production systems. Typically, cover crops are planted to improve soil fertility and the natural suppression of soilborne pests at times when fields...

  19. Florida Preservice Agricultural Education Teachers' Mathematics Ability and Efficacy

    Science.gov (United States)

    Stripling, Christopher T.; Roberts, T. Grady

    2012-01-01

    The purpose of this study was to examine the mathematics ability and efficacy of Florida preservice agricultural education teachers. Results indicated that the preservice teachers were not proficient in solving agricultural mathematics problems. On the other hand, the preservice teachers were efficacious in personal teaching efficacy and personal…

  20. University of West Florida Work Plan, 2013-2014

    Science.gov (United States)

    Board of Governors, State University System of Florida, 2013

    2013-01-01

    The State University System of Florida has developed three tools that aid in guiding the System's future: (1) The Board of Governors' new Strategic Plan 2012-2025 is driven by goals and associated metrics that stake out where the System is headed; (2) The Board's Annual Accountability Report provides yearly tracking for how the System is…

  1. 78 FR 77604 - Tomatoes Grown in Florida; Increased Assessment Rate

    Science.gov (United States)

    2013-12-24

    ...; ] DEPARTMENT OF AGRICULTURE Agricultural Marketing Service 7 CFR Part 966 Tomatoes Grown in Florida; Increased Assessment Rate AGENCY: Agricultural Marketing Service, USDA. ACTION: Proposed rule. SUMMARY: This proposed... ``order.'' The order is effective under the Agricultural Marketing Agreement Act of 1937, as amended (7...

  2. IPM of specialty crops and community gardens in north Florida

    Science.gov (United States)

    Insect pests post serious challenges to specialty crops (vegetables, fruits and nut crops) and community gardens in North Florida. The major vegetable pests include silverleaf whitefly, Bemisia argentifolii; the green peach aphid, Myzus persicae; southeastern green stinkbug, Nezara viridula; brown s...

  3. Estimation of the bottleneck size in Florida panthers

    Science.gov (United States)

    Culver, M.; Hedrick, P.W.; Murphy, K.; O'Brien, S.; Hornocker, M.G.

    2008-01-01

    We have estimated the extent of genetic variation in museum (1890s) and contemporary (1980s) samples of Florida panthers Puma concolor coryi for both nuclear loci and mtDNA. The microsatellite heterozygosity in the contemporary sample was only 0.325 that in the museum samples although our sample size and number of loci are limited. Support for this estimate is provided by a sample of 84 microsatellite loci in contemporary Florida panthers and Idaho pumas Puma concolor hippolestes in which the contemporary Florida panther sample had only 0.442 the heterozygosity of Idaho pumas. The estimated diversities in mtDNA in the museum and contemporary samples were 0.600 and 0.000, respectively. Using a population genetics approach, we have estimated that to reduce either the microsatellite heterozygosity or the mtDNA diversity this much (in a period of c. 80years during the 20th century when the numbers were thought to be low) that a very small bottleneck size of c. 2 for several generations and a small effective population size in other generations is necessary. Using demographic data from Yellowstone pumas, we estimated the ratio of effective to census population size to be 0.315. Using this ratio, the census population size in the Florida panthers necessary to explain the loss of microsatellite variation was c .41 for the non-bottleneck generations and 6.2 for the two bottleneck generations. These low bottleneck population sizes and the concomitant reduced effectiveness of selection are probably responsible for the high frequency of several detrimental traits in Florida panthers, namely undescended testicles and poor sperm quality. The recent intensive monitoring both before and after the introduction of Texas pumas in 1995 will make the recovery and genetic restoration of Florida panthers a classic study of an endangered species. Our estimates of the bottleneck size responsible for the loss of genetic variation in the Florida panther completes an unknown aspect of this

  4. Spatial epidemiology of eastern equine encephalitis in Florida

    Directory of Open Access Journals (Sweden)

    Vander Kelen Patrick T

    2012-11-01

    Full Text Available Abstract Background Eastern Equine Encephalitis virus (EEEV is an alphavirus with high pathogenicity in both humans and horses. Florida continues to have the highest occurrence of human cases in the USA, with four fatalities recorded in 2010. Unlike other states, Florida supports year-round EEEV transmission. This research uses GIS to examine spatial patterns of documented horse cases during 2005–2010 in order to understand the relationships between habitat and transmission intensity of EEEV in Florida. Methods Cumulative incidence rates of EEE in horses were calculated for each county. Two cluster analyses were performed using density-based spatial clustering of applications with noise (DBSCAN. The first analysis was based on regional clustering while the second focused on local clustering. Ecological associations of EEEV were examined using compositional analysis and Euclidean distance analysis to determine if the proportion or proximity of certain habitats played a role in transmission. Results The DBSCAN algorithm identified five distinct regional spatial clusters that contained 360 of the 438 horse cases. The local clustering resulted in 18 separate clusters containing 105 of the 438 cases. Both the compositional analysis and Euclidean distance analysis indicated that the top five habitats positively associated with horse cases were rural residential areas, crop and pastureland, upland hardwood forests, vegetated non-forested wetlands, and tree plantations. Conclusions This study demonstrates that in Florida tree plantations are a focus for epizootic transmission of EEEV. It appears both the abundance and proximity of tree plantations are factors associated with increased risk of EEE in horses and therefore humans. This association helps to explain why there is are spatially distinct differences in the amount of EEE horse cases across Florida.

  5. 77 FR 74923 - Water Quality Standards for the State of Florida's Estuaries, Coastal Waters, and South Florida...

    Science.gov (United States)

    2012-12-18

    ... of Costs F. Benefits VII. Statutory and Executive Order Reviews A. Executive Orders 12866 (Regulatory...), total phosphorus (TP) and chlorophyll a concentrations. EPA's methodology and the resulting proposed... Florida except the Big Bend Coastal region. Using this approach, EPA developed chlorophyll a criteria...

  6. Florida-focused climate change lesson demonstrations from the ASK Florida global and regional climate change professional development workshops

    Science.gov (United States)

    Weihs, R. R.

    2013-12-01

    A variety of Florida-focused climate change activities will be featured as part of the ASK Florida global and regional climate change professional development workshops. In a combined effort from Florida State University's Center for Ocean-Atmospheric Prediction Studies (COAPS) and University of South Florida's Coalition for Science Literacy (CSL), and supported by NASA's NICE initiative, the ASK Florida professional development workshops are a series of workshops designed to enhance and support climate change information and related pedagogical skills for middle school science teachers from Title-I schools in Florida. These workshops took place during a two-year period from 2011 to 2013 and consisted of two cohorts in Hillsborough and Volusia counties in Florida. Featured activities include lab-style exercises demonstrating topics such as storm surge and coastal geometry, sea level rise from thermal expansion, and the greenhouse effect. These types of labs are modified so that they allow more independent, inquiry thinking as they require teachers to design their own experiment in order to test a hypothesis. Lecture based activities are used to cover a broad range of topics including hurricanes, climate modeling, and sink holes. The more innovative activities are group activities that utilize roll-playing, technology and resources, and group discussion. For example, 'Climate Gallery Walk' is an activity that features group discussions on each of the climate literacy principles established by the United States Global Change Research Program. By observing discussions between individuals and groups, this activity helps the facilitators gather information on their previous knowledge and identify possible misconceptions that will be addressed within the workshops. Furthermore, 'Fact or Misconception' presents the challenge of identifying whether a given statement is fact or misconception based on the material covered throughout the workshops. It serves as a way to

  7. Management case study: Tampa Bay, Florida

    Science.gov (United States)

    Morrison, Gerold; Greening, Holly; Yates, Kimberly K.; Wolanski, Eric; McLusky, Donald S.

    2011-01-01

    Tampa Bay, Florida, USA, is a shallow, subtropical estuary that experienced severe cultural eutrophication between the 1940s and 1980s, a period when the human population of its watershed quadrupled. In response, citizen action led to the formation of a public- and private-sector partnership (the Tampa Bay Estuary Program), which adopted a number of management objectives to support the restoration and protection of the bay’s living resources. These included numeric chlorophyll a and water-clarity targets, as well as long-term goals addressing the spatial extent of seagrasses and other selected habitat types, to support estuarine-dependent faunal guilds. Over the past three decades, nitrogen controls involving sources such as wastewater treatment plants, stormwater conveyance systems, fertilizer manufacturing and shipping operations, and power plants have been undertaken to meet these and other management objectives. Cumulatively, these controls have resulted in a 60% reduction in annual total nitrogen (TN) loads relative to earlier worse-case (latter 1970s) conditions. As a result, annual water-clarity and chlorophyll a targets are currently met in most years, and seagrass cover measured in 2008 was the highest recorded since 1950. Factors that have contributed to the observed improvements in Tampa Bay over the past several decades include the following: (1) Development of numeric, science-based water-quality targets to meet a long-term goal of restoring seagrass acreage to 1950s levels. Empirical and mechanistic models found that annual average chlorophyll a concentrations were a primary manageable factor affecting light attenuation. The models also quantified relationships between TN loads, chlorophyll a concentrations, light attenuation, and fluctuations in seagrass cover. The availability of long-term monitoring data, and a systematic process for using the data to evaluate the effectiveness of management actions, has allowed managers to track progress and

  8. Conserved Noncoding Elements in the Most Distant Genera of Cephalochordates: The Goldilocks Principle

    Science.gov (United States)

    Yue, Jia-Xing; Kozmikova, Iryna; Ono, Hiroki; Nossa, Carlos W.; Kozmik, Zbynek; Putnam, Nicholas H.; Yu, Jr-Kai; Holland, Linda Z.

    2016-01-01

    Cephalochordates, the sister group of vertebrates + tunicates, are evolving particularly slowly. Therefore, genome comparisons between two congeners of Branchiostoma revealed so many conserved noncoding elements (CNEs), that it was not clear how many are functional regulatory elements. To more effectively identify CNEs with potential regulatory functions, we compared noncoding sequences of genomes of the most phylogenetically distant cephalochordate genera, Asymmetron and Branchiostoma, which diverged approximately 120–160 million years ago. We found 113,070 noncoding elements conserved between the two species, amounting to 3.3% of the genome. The genomic distribution, target gene ontology, and enriched motifs of these CNEs all suggest that many of them are probably cis-regulatory elements. More than 90% of previously verified amphioxus regulatory elements were re-captured in this study. A search of the cephalochordate CNEs around 50 developmental genes in several vertebrate genomes revealed eight CNEs conserved between cephalochordates and vertebrates, indicating sequence conservation over >500 million years of divergence. The function of five CNEs was tested in reporter assays in zebrafish, and one was also tested in amphioxus. All five CNEs proved to be tissue-specific enhancers. Taken together, these findings indicate that even though Branchiostoma and Asymmetron are distantly related, as they are evolving slowly, comparisons between them are likely optimal for identifying most of their tissue-specific cis-regulatory elements laying the foundation for functional characterizations and a better understanding of the evolution of developmental regulation in cephalochordates. PMID:27412606

  9. Characterization Of Dissolved Organic Mattter In The Florida Keys Ecosystem

    Science.gov (United States)

    Adams, D. G.; Shank, G. C.

    2009-12-01

    Over the past few decades, Scleractinian coral populations in the Florida Keys have increasingly experienced mortality due to bleaching events as well as microbial mediated illnesses such as black band and white band disease. Such pathologies seem to be most correlated with elevated sea surface temperatures, increased UV exposures, and shifts in the microbial community living on the coral itself. Recent studies indicate that corals’ exposure to UV in the Florida Keys is primarily controlled by the concentration of CDOM (Chromophoric Dissolved Organic Matter) in the water column. Further, microbial community alterations may be linked to changes in concentration and chemical composition of the larger DOM (Dissolved Organic Matter) pool. Our research characterized the spatial and temporal properties of DOM in Florida Bay and along the Keys ecosystems using DOC analyses, in-situ water column optical measurements, and spectral analyses including absorbance and fluorescence measurements. We analyzed DOM characteristics along transects running from the mouth of the Shark River at the southwest base of the Everglades, through Florida Bay, and along near-shore Keys coastal waters. Two 12 hour time-series samplings were also performed at the Seven-Mile Bridge, the primary Florida Bay discharge channel to the lower Keys region. Photo-bleaching experiments showed that the chemical characteristics of the DOM pool are altered by exposure to solar radiation. Results also show that DOC (~0.8-5.8 mg C/L) and CDOM (~0.5-16.5 absorbance coefficient at 305nm) concentrations exhibit seasonal fluctuations in our study region. EEM analyses suggest seasonal transitions between primarily marine (summer) and terrestrial (winter) sources along the Keys. We are currently combining EEM-PARAFAC analysis with in-situ optical measurements to model changes in the spectral properties of DOM in the water column. Additionally, we are using stable δ13C isotopic analysis to further characterize DOM

  10. Applying Best Practices to Florida Local Government Retrofit Programs, Central Florida (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    2014-03-01

    In some communities, local government and non-profit entities have funds to purchase and renovate distressed, foreclosed homes for resale in the affordable housing market. Numerous opportunities to improve whole house energy efficiency are inherent in these comprehensive renovations. BA-PIRC worked together in a multi-year field study making recommendations in individual homes, meanwhile compiling improvement costs, projected energy savings, practical challenges, and labor force factors surrounding common energy-related renovation measures. The field study, Phase 1 of this research, resulted in a set of best practices appropriate to the current labor pool and market conditions in central Florida to achieve projected annual energy savings of 15-30% and higher. This report describes Phase 2 of the work where researchers worked with a local government partner to implement and refine the "current best practices". A simulation study was conducted to characterize savings potential under three sets of conditions representing varying replacement needs for energy-related equipment and envelope components. The three scenarios apply readily to the general remodeling industry as for renovation of foreclosed homes for the affordable housing market. Our new local government partner, the City of Melbourne, implemented the best practices in a community-scale renovation program that included ten homes in 2012. ​

  11. Studies of nuclei using radioactive beams. [Space Astronomy Lab. , Univ. of Florida, Gainesville, Florida

    Energy Technology Data Exchange (ETDEWEB)

    Piercey, R.B.

    1989-07-01

    The 12 month period from May 1988 to July 1989 represents the first full year of our 18 month pilot program in nuclear structure research. In this period, research was initiated to develop a capability for radioactive secondary beams at Argonne National Laboratory using the Atlas and the new Fragment Mass Analyzer (FMA), which is currently under construction. Two major new detector facilities are currently in the final stages of design and testing. The Large-Area, Scintillator Telescope (LAST) detector is fully operational and will be shipped to Argonne National Laboratory in August for fit-tests and in-beam calibrations. The first segments of a new sixteen-segment neutron multiplicity detector have been built and tested. The remaining segments are currently being constructed. Research was continued in the areas of (1) Coulomb excitation studies of rare earth and actinide nuclei; (2) In-beam, gamma-ray spectroscopy of nuclei in the mass 100 region, and (3) Advanced detector design. Several journal articles and abstracts were published or submitted for publication in the reporting period, and others are currently in preparation. Three graduate students participated in the program, one from the University of Florida and two from the Royal Institute of Technology, Stockholm, Sweden.

  12. First report of a Florida Manatee (Trichechus manatus latirostris) in Cuba

    Science.gov (United States)

    Alvarez-Aleman, Anmari; Beck, Cathy A.; Powell, James A.

    2010-01-01

    Manatees (Trichechus manatus latirostris) in Florida utilize intake and effluent canals of power plants as resting and thermoregulatory habitat. We report the use of a power plant canal in Cuba by a known Florida manatee, the first documented case of movement by a manatee between Florida and Cuba. In January, February, and April 2007, two manatees (mother and calf) were reported entering a power plant canal in north Havana, Cuba. The larger manatee had several distinctive scars which were photographed. Digital images were matched to a previously known Florida manatee (CR131) with a sighting history dating from December 1979 to July 2006. Exchanges of individuals between Florida and Cuba may have important genetic implications, particularly since there appears to be little genetic exchange between the Florida manatee subspecies with populations of the Antillean manatee subspecies (T. m. manatus) in Puerto Rico and the Dominican Republic.

  13. Evolution of selenoproteins in the metazoan

    Directory of Open Access Journals (Sweden)

    Jiang Liang

    2012-09-01

    Full Text Available Abstract Background The selenocysteine (Sec containing proteins, selenoproteins, are an important group of proteins present throughout all 3 kingdoms of life. With the rapid progression of selenoprotein research in the post-genomic era, application of bioinformatics methods to the identification of selenoproteins in newly sequenced species has become increasingly important. Although selenoproteins in human and other vertebrates have been investigated, studies of primitive invertebrate selenoproteomes are rarely reported outside of insects and nematodes. Result A more integrated view of selenoprotein evolution was constructed using several representative species from different evolutionary eras. Using a SelGenAmic-based selenoprotein identification method, 178 selenoprotein genes were identified in 6 invertebrates: Amphimedon queenslandica, Trichoplax adhaerens, Nematostella vectensis, Lottia gigantean, Capitella teleta, and Branchiostoma floridae. Amphioxus was found to have the most abundant and variant selenoproteins of any animal currently characterized, including a special selenoprotein P (SelP possessing 3 repeated Trx-like domains and Sec residues in the N-terminal and 2 Sec residues in the C-terminal. This gene structure suggests the existence of two different strategies for extension of Sec numbers in SelP for the preservation and transportation of selenium. In addition, novel eukaryotic AphC-like selenoproteins were identified in sponges. Conclusion Comparison of various animal species suggests that even the most primitive animals possess a selenoproteome range and variety similar to humans. During evolutionary history, only a few new selenoproteins have emerged and few were lost. Furthermore, the massive loss of selenoproteins in nematodes and insects likely occurred independently in isolated partial evolutionary branches.

  14. Hox gene clusters in the Indonesian coelacanth, Latimeria menadoensis.

    Science.gov (United States)

    Koh, Esther G L; Lam, Kevin; Christoffels, Alan; Erdmann, Mark V; Brenner, Sydney; Venkatesh, Byrappa

    2003-02-01

    The Hox genes encode transcription factors that play a key role in specifying body plans of metazoans. They are organized into clusters that contain up to 13 paralogue group members. The complex morphology of vertebrates has been attributed to the duplication of Hox clusters during vertebrate evolution. In contrast to the single Hox cluster in the amphioxus (Branchiostoma floridae), an invertebrate-chordate, mammals have four clusters containing 39 Hox genes. Ray-finned fishes (Actinopterygii) such as zebrafish and fugu possess more than four Hox clusters. The coelacanth occupies a basal phylogenetic position among lobe-finned fishes (Sarcopterygii), which gave rise to the tetrapod lineage. The lobe fins of sarcopterygians are considered to be the evolutionary precursors of tetrapod limbs. Thus, the characterization of Hox genes in the coelacanth should provide insights into the origin of tetrapod limbs. We have cloned the complete second exon of 33 Hox genes from the Indonesian coelacanth, Latimeria menadoensis, by extensive PCR survey and genome walking. Phylogenetic analysis shows that 32 of these genes have orthologs in the four mammalian HOX clusters, including three genes (HoxA6, D1, and D8) that are absent in ray-finned fishes. The remaining coelacanth gene is an ortholog of hoxc1 found in zebrafish but absent in mammals. Our results suggest that coelacanths have four Hox clusters bearing a gene complement more similar to mammals than to ray-finned fishes, but with an additional gene, HoxC1, which has been lost during the evolution of mammals from lobe-finned fishes.

  15. Characterization of the neurohypophysial hormone gene loci in elephant shark and the Japanese lamprey: origin of the vertebrate neurohypophysial hormone genes

    Directory of Open Access Journals (Sweden)

    Brenner Sydney

    2009-02-01

    Full Text Available Abstract Background Vasopressin and oxytocin are mammalian neurohypophysial hormones with distinct functions. Vasopressin is involved mainly in osmoregulation and oxytocin is involved primarily in parturition and lactation. Jawed vertebrates contain at least one homolog each of vasopressin and oxytocin, whereas only a vasopressin-family hormone, vasotocin, has been identified in jawless vertebrates. The genes encoding vasopressin and oxytocin are closely linked tail-to-tail in eutherian mammals whereas their homologs in chicken, Xenopus and coelacanth (vasotocin and mesotocin are linked tail-to-head. In contrast, their pufferfish homologs, vasotocin and isotocin, are located on the same strand of DNA with isotocin located upstream of vasotocin and separated by five genes. These differences in the arrangement of the two genes in different bony vertebrate lineages raise questions about their origin and ancestral arrangement. To trace the origin of these genes, we have sequenced BAC clones from the neurohypophysial gene loci in a cartilaginous fish, the elephant shark (Callorhinchus milii, and in a jawless vertebrate, the Japanese lamprey (Lethenteron japonicum. We have also analyzed the neurohypophysial hormone gene locus in an invertebrate chordate, the amphioxus (Branchiostoma floridae. Results The elephant shark neurohypophysial hormone genes encode vasotocin and oxytocin, and are linked tail-to-head like their homologs in coelacanth and non-eutherian tetrapods. Besides the hypothalamus, the two genes are also expressed in the ovary. In addition, the vasotocin gene is expressed in the kidney, rectal gland and intestine. These expression profiles indicate a paracrine role for the two hormones. The lamprey locus contains a single neurohypophysial hormone gene, the vasotocin. The synteny of genes in the lamprey locus is conserved in elephant shark, coelacanth and tetrapods but disrupted in teleost fishes. The amphioxus locus encodes a single

  16. Hydrologic Conditions in Florida during Water Year 2007

    Science.gov (United States)

    Verdi, Richard Jay; Tomlinson, Stewart A.; Irvin, Ronald B.; Fulcher, David L.

    2009-01-01

    Record-high and record-low hydrologic conditions occurred during water year 2007 (October 1, 2006 - September 30, 2007) based on analyses of precipitation, surface-water flows, lake elevations, and ground-water levels. For example, the streamgage at Suwannee River at White Springs in northwest Florida recorded an annual streamflow of 103 cubic feet per second during 2007, or about 6 percent of the period-of-record average since monitoring began in 1906. Lake Okeechobee in south Florida reached record-low elevations (8.82 feet on July 2) since monitoring began in 1912. Several wells throughout the State registered period-of-record lowest daily maximum water levels.

  17. Ecology of the mangroves of south Florida: a community profile

    Energy Technology Data Exchange (ETDEWEB)

    Odum, W.E.; McIvor, C.C.; Smith, T.J. III

    1982-01-01

    A detailed description is given of the community structure and ecosystem processes of the mangrove forests of south Florida. This description is based upon a compilation of data and hypotheses from published and unpublished sources. Information covered ranges from details of mangrove distribution, primary production, and diseases to asepcts of reproduction, biomass partitioning, and adaptations to stress. Mangrove ecosystems are considered in terms of zonation, succession, litter fall and decomposition, carbon export, and energy flow. Most of the components of mangrove communities are cataloged and discussed; these include mircoorganisms, plants other than mangroves, invertebrates, fishes, reptiles, amphibians, birds, and mammals. Finally, two sections summarize the value of mangrove ecosystems to man and present ways to manage this type of habitat. It is concluded that mangrove forests, which cover between 430,000 and 500,000 acres (174,000 to 202,000 ha) in Florida, are a resource of great value and should be protected and preserved wherever possible.

  18. Regional flood-frequency relations for west-central Florida

    Science.gov (United States)

    Seijo, M.A.; Giovannelli, R.F.; Turner, J.F.

    1979-01-01

    This report presents regional relations for estimating the magnitude and frequency of floods on streams in west-central Florida. Flood prediction equations derived cover 20, 5-, 25-, 100-, 200-, and 500-year recurrence intervals. Annual floods for three geographic areas of west-central Florida were found to relate significantly to basin characteristics. Basin characteristics include drainage area, soils index, slope, and lake area. The average standard error of estimate for regional flood relations ranged from 38.4 to 52.1 percent with a mean of 43.5 percent. The average multiple correlation coefficient if 0.94. Regional relations apply to gaged and ungaged sites whose drainage areas are greater than 10 but less than 2,500 square miles. Tables of maximum known floods for 64 streamflow stations used in the analysis are included. Tables comparing station, weighted, and regional flood-peak discharges are also included. (Kosco-USGS)

  19. PROS AND CONS OF P. FLORIDA CULTIVATION FOR MANAGING WASTE OF HANDMADE PAPER AND CARDBOARD INDUSTRIES

    OpenAIRE

    Shweta Kulshreshtha, Nupur Mathur, and Pradeep Bhatnagar

    2011-01-01

    The possibility of utilizing handmade paper and cardboard industrial sludges in the production of edible mushrooms involves risk of introducing toxic substances into the human food chain. Therefore, in the present study, genotoxic assessment of P. florida (Pleurotus florida) cultivated on these industrial sludges and their combination with wheat straw was done by Ames test using Salmonella typhimurium TA 98 and TA100. Interestingly, P. florida carpophores, cultivated on wheat straw did not sh...

  20. Horizontal Nearshore Surface Dispersion for the Florida Panhandle

    Science.gov (United States)

    2014-06-01

    NEARSHORE SURFACE DISPERSION FOR THE FLORIDA PANHANDLE by Kate J. Woodall June 2014 Thesis Advisor: James MacMahan Second Reader: Edward...followed regime, with λ results exhibiting enhanced growth over scales < 100 m, with Richardson like growth, λ ~ δ-2/3, for scales > 100 m. It is...OCEANOGRAPHY from the NAVAL POSTGRADUATE SCHOOL June 2014 Author: Kate J. Woodall Approved by: James MacMahan, Ph.D. Thesis

  1. INSPECTION TECHNOLOGY, DETECTION, AND COMPLIANCE: EVIDENCE FROM FLORIDA RESTAURANT INSPECTIONS

    OpenAIRE

    Jin, Ginger Zhe; Lee, Jungmin

    2014-01-01

    In this article, we show that a small innovation in inspection technology can make substantial differences in inspection outcomes. For restaurant hygiene inspections, the state of Florida has introduced a handheld electronic device, the portable digital assistant (PDA), which reminds inspectors of 1,000 potential violations that may be checked for. Using inspection records from July 2003 to June 2009, we find that the adoption of PDA led to 11% more detected violations and subsequently restau...

  2. Remarkable movements of an American crocodile (Crocodylus acutus) in Florida

    Science.gov (United States)

    Cherkiss, Michael S.; Mazzotti, Frank J.; Hord, Lindsey; Aldecoa, Mario

    2014-01-01

    Here we present the remarkable movements of an individual Crocodylus acutus (American Crocodile) over a 14-year period. The crocodile was originally marked in Homestead, FL as a young-of-the-year in 1999, and was later recaptured multiple times more than 388 km away along the southwest coast of Florida. After several relocations and numerous sightings, this individual who has become known as Yellow Number 1 was found back within the same canal system in which it was first captured.

  3. Archaeological investigations in Palanda, Santa Ana-La Florida (Ecuador)

    OpenAIRE

    Valdez, Francisco

    2014-01-01

    International audience; Recent archaeological research carried out in Zamora Chinchipe (Ecuador) have shown the presence of a previously unknown prehispanic culture, that was present throughout the Mayo Chinchipe-Marañón hydraulic basin; thus it was designated with the same name. Archaeologial work in the Santa Ana-La Florida site (Palanda) has given a precise chronological dimension and has produced evidence on the ideology, architecture, funerary customs and the material culture in general ...

  4. Florida's salt-marsh management issues: 1991-98.

    Science.gov (United States)

    Carlson, D B; O'Bryan, P D; Rey, J R

    1999-06-01

    During the 1990s, Florida has continued to make important strides in managing salt marshes for both mosquito control and natural resource enhancement. The political mechanism for this progress continues to be interagency cooperation through the Florida Coordinating Council on Mosquito Control and its Subcommittee on Managed Marshes (SOMM). Continuing management experience and research has helped refine the most environmentally acceptable source reduction methods, which typically are Rotational Impoundment Management or Open Marsh Water Management. The development of regional marsh management plans for salt marshes within the Indian River Lagoon by the SOMM has helped direct the implementation of the best management practices for these marshes. Controversy occasionally occurs concerning what management technique is most appropriate for individual marshes. The most common disagreement is over the benefits of maintaining an impoundment in an "open" vs. "closed" condition, with the "closed" condition, allowing for summer mosquito control flooding or winter waterfowl management. New federal initiatives influencing salt-marsh management have included the Indian River Lagoon-National Estuary Program and the Pesticide Environmental Stewardship Program. A new Florida initiative is the Florida Department of Environmental Protection's Eco-system Management Program with continuing involvement by the Surface Water Improvement and Management program. A developing mitigation banking program has the potential to benefit marsh management but mosquito control interests may suffer if not handled properly. Larvicides remain as an important salt-marsh integrated pest management tool with the greatest acreage being treated with temephos, followed by Bacillus thuringiensis israelensis and methoprene. However, over the past 14 years, use of biorational larvicides has increased greatly.

  5. The Stocker AstroScience Center at Florida International University

    Science.gov (United States)

    Webb, James R.

    2014-01-01

    The new Stocker AstroScience Center located on the MMC campus at Florida International University in Miami Florida represents a unique facility for STEM education that arose from a combination of private, State and university funding. The building, completed in the fall of 2013, contains some unique spaces designed not only to educate, but also to inspire students interested in science and space exploration. The observatory consists of a 4-story building (3 floors) with a 24” ACE automated telescope in an Ash dome, and an observing platform above surrounding buildings. Some of the unique features of the observatory include an entrance/exhibition hall with a 6-ft glass tile floor mural linking the Florida climate to space travel, a state-of-the art telescope control that looks like a starship bridge, and displays such as “Music from the universe”. The observatory will also be the focus of our extensive public outreach program that is entering its 20 year.

  6. Echocardiographic evaluation of clinically healthy Florida manatees (Trichechus manatus latirostris).

    Science.gov (United States)

    Gerlach, Trevor J; Estrada, Amara H; Sosa, Ivan S; Powell, Melanie; Maisenbacher, Herbert W; de Wit, Martine; Ball, Ray L; Walsh, Michael T

    2013-06-01

    Antemortem studies pertaining to the manatee cardiovascular and cardiopulmonary systems are limited despite reports of cardiac disease in postmortem specimens. The objective of this project was to develop a technique for echocardiography in the Florida manatee (Trichechus manatus latirostris). Because of their unique anatomy, a ventral approach was employed by use of an echocardiography table designed specifically for this study. Fourteen clinically healthy, free-ranging and captive Florida manatees underwent echocardiography between the fall of 2011 and winter of 2012. Eight females and six males of various age categories were included in the study. Clear visualization of all valves and chambers was accomplished, and length and width measurements of the left atrium, peak aortic flow velocity, and ejection fraction percentage were calculated in most animals. Abnormalities observed during the study included atrioventricular regurgitation and severe right-atrial enlargement. Based on the results of this study, echocardiography in the Florida manatee is possible, which has both clinical and research implications in larger epidemiologic studies evaluating diseases of the cardiopulmonary and cardiovascular systems.

  7. A data-based conservation planning tool for Florida panthers

    Science.gov (United States)

    Murrow, Jennifer L.; Thatcher, Cindy A.; Van Manen, Frank T.; Clark, Joseph D.

    2013-01-01

    Habitat loss and fragmentation are the greatest threats to the endangered Florida panther (Puma concolor coryi). We developed a data-based habitat model and user-friendly interface so that land managers can objectively evaluate Florida panther habitat. We used a geographic information system (GIS) and the Mahalanobis distance statistic (D2) to develop a model based on broad-scale landscape characteristics associated with panther home ranges. Variables in our model were Euclidean distance to natural land cover, road density, distance to major roads, human density, amount of natural land cover, amount of semi-natural land cover, amount of permanent or semi-permanent flooded area–open water, and a cost–distance variable. We then developed a Florida Panther Habitat Estimator tool, which automates and replicates the GIS processes used to apply the statistical habitat model. The estimator can be used by persons with moderate GIS skills to quantify effects of land-use changes on panther habitat at local and landscape scales. Example applications of the tool are presented.

  8. Elementary particle physics at the University of Florida. Annual report

    Energy Technology Data Exchange (ETDEWEB)

    Field, R.D.; Ramond, P.M.; Sikivie, P. [and others

    1995-12-01

    This is the annual progress report of the University of Florida`s elementary particle physics group. The theoretical high energy physics group`s research covers a broad range of topics, including both theory and phenomenology. Present work of the experimental high energy physics group is directed toward the CLEO detector, with some effort going to B physics at Fermilab. The Axion Search project is participating in the operation of a large-scale axion detector at Lawrence Livermore National Laboratory, with the University of Florida taking responsibility for this experiment`s high-resolution spectrometer`s assembly, programming, and installation, and planning to take shifts during operation of the detector in FY96. The report also includes a continuation of the University`s three-year proposal to the United States Department of Energy to upgrade the University`s high-energy physics computing equipment and to continue student support, system manager/programmer support, and maintenance. Report includes lists of presentations and publications by members of the group.

  9. Paleogeographic Study of the West Florida Panhandle Coast and Margin

    Science.gov (United States)

    Koch, J. L.; Donoghue, J. F.; Niedoroda, A. W.; Hatchett, L.; Clark, R. R.

    2001-12-01

    The dominant factors in the evolution of a passive margin shelf and coast are sea-level change and fluvial-marine sediment transport processes. On many U.S. coasts, beach nourishment sand has become an increasingly scarce and expensive resource. A regional sand search has recently been undertaken to identify offshore targets for beach nourishment sand along the western Florida Panhandle coast. A major task of the project has been the development of a conceptual model for finding potential nourishment sand. The modeling work has involved the collection of existing data, including published literature, sediment samples, sub-bottom seismic data, and paleogeographic analyses. Paleogeography was recognized as a potentially powerful tool for use in identifying shelf sand bodies, because they are products of sea-level change and shelf evolution. As part of the project, a more detailed study has been undertaken to acquire and assemble all available paleogeographic and paleoshoreline data for the western Florida shelf. These data include studies of the Quaternary paleogeography of the Panhandle coast, still-stand paleoshorelines, high-resolution bathymetry, global and eustatic sea-level curves, beach ridge systems, coastal river and inlet retreat paths, and barrier island evolution. The data have been compiled into a Geographic Information System (GIS) database from which maps of the shelf paleogeography can be created, representing selected periods in the Quaternary evolution of the West Florida Panhandle coast and margin.

  10. Monitoring cryptic amphibians and reptiles in a Florida state park.

    Science.gov (United States)

    Engeman, Richard M; Meshaka, Walter E; Severson, Robert; Severson, Mary Ann; Kaufman, Greg; Groninger, N Paige; Smith, Henry T

    2016-04-01

    We monitored cryptic herpetofauna at Savannas Preserve State Park, Florida, by combining artificial cover counts with a quantitative paradigm for constructing and calculating population indices. Weekly indices were calculated from two consecutive days of data collection each week for 7 months from mid-winter to mid-summer in three habitats. Seventeen species were observed at least once, and time trends using index values were followed for six species. Among these, abundance and seasonal pattern information were obtained for an exotic species (greenhouse frog) and a species identified by the Florida Committee on Rare and Endangered Plants and Animals as threatened (Florida scrub lizard). We identified winter as the optimal time in this area to monitor populations for conducting annual assessments. This combined observation and indexing approach could provide managers or researchers with an economical means to quantitatively index population trends for multiple cryptic herpetofauna species simultaneously. Using artificial cover to sample within a population indexing design can be generalized beyond monitoring herpetofauna. Other forms of artificial cover that can be used as observation stations include aquatic artificial substrates, artificial tree cavities, artificial reefs, and other artificial aquatic structures and artificial sea grass units, among many others, and a wide range of taxa are suitable for population monitoring using artificial cover as observation stations in the approach we present, including insects, soil invertebrates, micro and macro aquatic invertebrates, fish, crustaceans, and small mammals.

  11. Reproductive seasonality of the female Florida gar, Lepisosteus platyrhincus.

    Science.gov (United States)

    Orlando, Edward F; Binczik, Gerald A; Denslow, Nancy D; Guillette, Louis J

    2007-05-01

    Our objective was to characterize the reproductive seasonality of a wild population of female Florida gar, Lepisosteus platyrhincus. We measured the gonadosomatic index, plasma estradiol, testosterone, and vitellogenin concentrations, and follicle diameters of fish collected from Orange Lake, Florida (USA). Additionally, the reproductive stage of the ovary was resolved histologically. We observed a seasonal pattern in reproduction. Following a quiescent period in the summer, there was an increase in sex steroid hormones during the fall, associated with the onset of vitellogenesis and active oogenesis. During the February collection, we directly observed ovulation in several females. This observation was supported by a sharp decrease in the gonadosomatic index between February and March as well as a decline in plasma hormones to basal levels during the summer months. Our data suggest the Florida gar has a group-synchronous ovary and the majority of females spawned during the early spring, followed by a general decrease in reproductive parameters to summer levels. While hormone and vitellogenin peaks may vary annually and variations in these reproductive patterns are expected for different collection sites, this study is important as it is the first characterization of the seasonal morphological and endocrinological reproductive pattern in a female holostean species. Moreover, it increases our understanding of basic reproductive biology of semitropical fishes in general, and nonteleost, bony fishes in particular.

  12. Further psychometric analysis of the Florida Obsessive-Compulsive Inventory.

    Science.gov (United States)

    Aldea, Mirela A; Geffken, Gary R; Jacob, Marni L; Goodman, Wayne K; Storch, Eric A

    2009-01-01

    This study examined the psychometric properties of the Florida Obsessive-Compulsive Inventory (FOCI [Storch, E. A., Stigge-Kaufman, D., Bagner, D., Merlo, L. J., Shapira, N. A., Geffken, G. R., et al. (2007). Florida Obsessive-Compulsive Scale: development, reliability, and validity. Journal of Clinical Psychology, 63, 851-859]). Participants were 89 adults with OCD presenting for treatment at a specialty clinic. A trained clinician administered the Yale-Brown Obsessive Compulsive Scale and patients completed the FOCI, Obsessive-Compulsive Inventory-Revised, Beck Depression Inventory-Second Edition, and State Trait Anxiety Inventory at baseline and following 14 weekly or daily cognitive behavioral therapy sessions. The internal consistency of FOCI Symptom Checklist and Severity Scale were good, and the concurrent and divergent validity of the FOCI Symptom Checklist and Severity Scale was supported through its associations with clinician-rated OCD symptom severity, and self-reported OCD, depressive, and anxiety measures. In addition, sensitivity to cognitive-behavioral treatment effects was shown as the Severity Scale scores were significantly lower following treatment compared to baseline. These findings not only replicate Storch et al. [Storch, E. A., Stigge-Kaufman, D., Bagner, D., Merlo, L. J., Shapira, N. A., Geffken, G. R., et al. (2007). Florida Obsessive-Compulsive Scale: development, reliability, and validity. Journal of Clinical Psychology, 63, 851-859] but also add unique construct validity data in support of the psychometrics of the FOCI.

  13. Supreme Court to hear Florida clinic access case.

    Science.gov (United States)

    1994-04-15

    On April 27, 1994, the US Supreme Court will review a Florida Supreme Court decision ensuring access to women's health clinics that offer abortion services. In October 1993, the Florida High Court determined that an order issued by Brevard/Seminole County Circuit Judge Robert McGregor that requires anti-abortion protestors to remain 36 feet from the clinic grounds, prohibits approaching any clinic patient within 300 feet of the facility, bars excessive noise during clinic hours, and creates a 300 foot safety zone around the homes of clinic staff was reasonable. Anti-abortion activists had challenged Judge McGregor's injunction, maintaining that it violated their First Amendment rights and was overboard. Days before the Florida High Court ruling, however, the US Court of Appeals for the Eleventh Circuit had rejected Judge McGregor's injunction as unconstitutional. Although the appeal to the US Supreme Court, Madsen vs Women's Health Center, was filed by anti-abortion activists, pro-choice groups are supporting the review as a means of resolving the confusion created by conflicting state and federal rulings.

  14. 77 FR 38273 - Availability of Seats for the Florida Keys National Marine Sanctuary Advisory Council

    Science.gov (United States)

    2012-06-27

    .../Scale (alternate), South Florida Ecosystem Restoration (alternate), Submerged Cultural Resources (member), Submerged Cultural Resources (alternate), Tourism--Upper Keys (member), and Tourism--Upper Keys...

  15. Land Use and Land Cover - BROWNFIELD AREAS IN FLORIDA - APRIL 2012

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This data set contains Brownfield Boundaries. Brownfields are defined by the Florida Department of Environmental Protection (FDEP) as abandoned, idled, or underused...

  16. Anatomia foliar de Eugenia florida DC. (Myrtaceae Foliar anatomy of Eugenia florida DC. (Myrtaceae

    Directory of Open Access Journals (Sweden)

    Ana Maria Donato

    2009-09-01

    Full Text Available Foi feito o estudo anatômico da folha de Eugenia florida DC., espécie arbórea da família Myrtaceae, coletada no Campus da Fundação Oswaldo Cruz, Rio de Janeiro, RJ. A espécie apresenta importantes propriedades farmacológicas, incluindo-se atividade antiviral. O presente estudo teve como objetivo fornecer dados, revelados através da microscopia óptica e da microscopia eletrônica de varredura, que possam contribuir para o conhecimento da espécie e, conseqüentemente, para a segurança em sua identificação. Anatomicamente, a folha é hipostomática, com organização dorsiventral do mesofilo. Apresenta tricomas simples apenas sobre a nervura mediana da face adaxial. As células epidérmicas apresentam contorno sinuoso em vista frontal e cutícula estriada. O parênquima paliçádico destaca-se pela grande quantidade de cristais prismáticos de oxalato de cálcio. Em posição subepidérmica ocorrem cavidades secretoras de óleos essenciais, pouco numerosas, nas duas faces da lâmina foliar. As células epidérmicas situadas sobre as estruturas secretoras constituem característica de valor diagnóstico e são reconhecíveis pela célula de topo, que é reniforme, circundada pelas adjacentes, que apresentam disposição radiada. A comparação entre folhas de sol e de sombra revela que, nas primeiras, as estruturas secretoras são completamente diferenciadas, ao contrário das folhas de sombra, além de apresentarem maior concentração de compostos ergásticos.A study of the foliar anatomy of Eugenia florida DC., a tree of Myrtaceae family collected at Fundação Oswaldo Cruz, Rio de Janeiro, RJ, was accomplished. This species presents important pharmacological properties, including antiviral activity. The aim of this research was to furnish data, revealed by optical microscopy and scanning electronically microscopy, in order to contribute to the knowledge of the species and to its safe identification. Anatomically, the leaf is

  17. Fishery gear interactions from stranded bottlenose dolphins, Florida manatees and sea turtles in Florida, U.S.A.

    Science.gov (United States)

    Adimey, Nicole M; Hudak, Christine A; Powell, Jessica R; Bassos-Hull, Kim; Foley, Allen; Farmer, Nicholas A; White, Linda; Minch, Karrie

    2014-04-15

    Documenting the extent of fishery gear interactions is critical to wildlife conservation efforts, especially for reducing entanglements and ingestion. This study summarizes fishery gear interactions involving common bottlenose dolphins (Tursiops truncatus truncatus), Florida manatees (Trichechus manatus latirostris) and sea turtles: loggerhead (Caretta caretta), green turtle (Chelonia mydas), leatherback (Dermochelys coriacea), hawksbill (Eretmochelys imbricata), Kemp's ridley (Lepidochelys kempii), and olive ridley (Lepidochelys olivacea) stranding in Florida waters during 1997-2009. Fishery gear interactions for all species combined were 75.3% hook and line, 18.2% trap pot gear, 4.8% fishing nets, and 1.7% in multiple gears. Total reported fishery gear cases increased over time for dolphins (pstrandings relative to total strandings for loggerhead sea turtles increased (p<0.05). Additionally, life stage and sex patterns were examined, fishery gear interaction hotspots were identified and generalized linear regression modeling was conducted.

  18. Temperature, salinity, photosynthetically active radiation and weather parameters at SEAKEYS stations in the Florida Keys, 2007 (NODC Accession 0058101)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains data collected at several Coastal-Marine Automated Network (C-MAN) stations in the Florida Keys National Marine Sanctuary and Florida Bay....

  19. Temperature, salinity, photosynthetically active radiation and weather parameters at SEAKEYS stations in the Florida Keys, 2003 (NODC Accession 0058097)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains data collected at several Coastal-Marine Automated Network (C-MAN) stations in the Florida Keys National Marine Sanctuary and Florida Bay....

  20. Temperature, salinity, photosynthetically active radiation and weather parameters at SEAKEYS stations in the Florida Keys, 2005 (NODC Accession 0058099)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains data collected at several Coastal-Marine Automated Network (C-MAN) stations in the Florida Keys National Marine Sanctuary and Florida Bay....

  1. Temperature, salinity, photosynthetically active radiation and weather parameters at SEAKEYS stations in the Florida Keys, 2006 (NODC Accession 0058100)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains data collected at several Coastal-Marine Automated Network (C-MAN) stations in the Florida Keys National Marine Sanctuary and Florida Bay....

  2. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Florida Panhandle: T_MAMMAL (Terrestrial Mammal Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for beach mice, red wolf, and Florida black bear for the Florida Panhandle. Vector polygons in this data...

  3. Temperature, salinity, photosynthetically active radiation and weather parameters at SEAKEYS stations in the Florida Keys, 2004 (NODC Accession 0058098)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This accession contains data collected at several Coastal-Marine Automated Network (C-MAN) stations in the Florida Keys National Marine Sanctuary and Florida Bay....

  4. Photosynthetically active and ultraviolet radiation at SEAKEYS station Molasses Reef (MLRF) in the Florida Keys, 2011 (NODC Accession 0098080)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The Florida Institute of Oceanography's (FIO) SEAKEYS (Sustained Ecological Research Related to Management of the Florida Keys Seascape) program began in 1989 and...

  5. Florida TeleHealth Summit: “Telemedicine –It’s Here, It’s Now”

    Directory of Open Access Journals (Sweden)

    Samantha Wainright Haas

    2015-07-01

    Full Text Available The Southeastern Telehealth Resource Center, Florida State University College of Medicine, and the Florida Partnership for TeleHealth invites participation in the 2nd Annual Florida TeleHealth Summit. The Summit will be held December 3-4, 2015 at the Alfond Inn, Winter Park, Florida. For further information about the Southeastern Telehealth Resource Center and the Summit, visit: http://www.setrc.us/

  6. Water withdrawals, use, and trends in Florida, 2010

    Science.gov (United States)

    Marella, Richard L.

    2014-01-01

    In 2010, the total amount of water withdrawn in Florida was estimated to be 14,988 million gallons per day (Mgal/d). Saline water accounted for 8,589 Mgal/d (57 percent) and freshwater accounted for 6,399 Mgal/d (43 percent). Groundwater accounted for 4,166 Mgal/d (65 percent) of freshwater withdrawals, and surface water accounted for the remaining 2,233 Mgal/d (35 percent). Surface water accounted for nearly all (99.9 percent) saline-water withdrawals. An additional 659 Mgal/d of reclaimed wastewater was used in Florida during 2010. Freshwater withdrawals were greatest in Palm Beach County (707 Mgal/d), and saline-water withdrawals were greatest in Hillsborough County (1,715 Mgal/d). Fresh groundwater provided drinking water (public supplied and self-supplied) for 17.33 million people (92 percent of Florida’s population), and fresh surface water provided drinking water for 1.47 million people (8 percent). The statewide public-supply gross per capita use for 2010 was 134 gallons per day, whereas the statewide public-supply domestic per capita use was 85 gallons per day. The majority of groundwater withdrawals (almost 62 percent) in 2010 were obtained from the Floridan aquifer system, which is present throughout most of the State. The majority of fresh surface-water withdrawals (56 percent) came from the southern Florida hydrologic unit subregion and is associated with Lake Okeechobee and the canals in the Everglades Agricultural Area of Glades, Hendry, and Palm Beach Counties, as well as the Caloosahatchee River and its tributaries in the agricultural areas of Collier, Glades, Hendry, and Lee Counties. Overall, agricultural irrigation accounted for 40 percent of the total freshwater withdrawals (ground and surface), followed by public supply with 35 percent. Public supply accounted for 48 percent of groundwater withdrawals, followed by agricultural self-supplied (34 percent), commercial-industrial-mining self-supplied (7 percent), recreational

  7. Water Withdrawals, Use, and Trends in Florida, 2005

    Science.gov (United States)

    Marella, Richard L.

    2009-01-01

    In 2005, the total amount of water withdrawals in Florida was estimated at 18,359 million gallons per day (Mgal/d). Saline water accounted for 11,486 Mgal/d (63 percent), and freshwater accounted for 6,873 Mgal/d (37 percent). Groundwater accounted for 4,247 Mgal/d (62 percent) of freshwater withdrawals, and surface water accounted for the remaining 2,626 Mgal/d (38 percent). Surface water accounted for nearly all (99.9 percent) saline-water withdrawals. An additional 660 Mgal/d of reclaimed wastewater was used in Florida during 2005. The largest amount of freshwater was withdrawn from Palm Beach County, and the largest amount of saline water was withdrawn from Pasco County. Fresh groundwater provided drinking water (public supplied and self-supplied) for 16.19 million people (90 percent of Florida's population), and fresh surface water provided drinking water for 1.73 million people (10 percent). The majority of groundwater withdrawals (nearly 60 percent) in 2005 was obtained from the Floridan aquifer system which is present throughout the entire State. The majority of fresh surface-water withdrawals (59 percent) came from the southern Florida hydrologic unit subregion and is associated with Lake Okeechobee and the canals in the Everglades Agricultural Area of Glades, Hendry, and Palm Beach Counties, as well as the Caloosahatchee River and its tributaries in the agricultural areas of Collier, Glades, Hendry, and Lee Counties. Overall, agricultural irrigation accounted for 40 percent of the total freshwater withdrawals (ground and surface), followed by public supply with 37 percent. Public supply accounted for 52 percent of groundwater withdrawals, followed by agricultural self-supplied (31 percent), ommercial-industrial-mining self-supplied (8.5 percent), recreational irrigation and domestic self-supplied (4 percent each), and power generation (0.5 percent). Agricultural self-supplied accounted for 56 percent of fresh surface-water withdrawals, followed by power

  8. Building America Case Study: Pilot Demonstration of Phased Energy Efficiency Retrofits: Deep Retrofits, Central and South Florida

    Energy Technology Data Exchange (ETDEWEB)

    2017-02-22

    The Florida Solar Energy Center (FSEC), in collaboration with Florida Power & Light (FPL), is pursuing a phased residential energy-efficiency retrofit program in Florida. Researchers are looking to establish the impacts of technologies of two retrofit packages -- shallow and deep -- on annual energy and peak energy reductions.

  9. 77 FR 74449 - Water Quality Standards for the State of Florida's Lakes and Flowing Waters; Proposed Rule; Stay

    Science.gov (United States)

    2012-12-14

    ... AGENCY 40 CFR Part 131 RIN 2040-AF41 Water Quality Standards for the State of Florida's Lakes and Flowing... regulation the ``Water Quality Standards for the State of Florida's Lakes and Flowing Waters; Final Rule... Information Does this action apply to me? Citizens concerned with water quality in Florida may be...

  10. Motivating Factors of Florida Community and State College Information Technology Faculty

    Science.gov (United States)

    Payne, Wendy Louise

    2013-01-01

    In this study the core job characteristics that contribute to the internal motivational factors and job satisfaction of information technology faculty members working at a community or state college in Florida were investigated. Fifty-four information technology faculty members working at a community or state college in Florida completed the Job…

  11. 75 FR 54400 - Florida Power and Light Company; Establishment of Atomic Safety and Licensing Board

    Science.gov (United States)

    2010-09-07

    ... COMMISSION Florida Power and Light Company; Establishment of Atomic Safety and Licensing Board Pursuant to...), and the Commission's regulations, see 10 CFR 2.104, 2.300, 2.303, 2.309, 2.311, 2.318, and 2.321... over the following proceeding: Florida Power & Light Company (Turkey Point Units 6 and 7)...

  12. Why Should I Go? Florida Teachers' Perceptions of Value in ELL Professional Development

    Science.gov (United States)

    Phillips, Jennifer J.

    2013-01-01

    Florida has undergone a 27.8% increase of ELL PreK-12 public school students within the last twenty years (www.fldoe/aala). Of the total student population in Florida, 9.4% speak 230 different languages and dialects with the highest percentage in Hispanic speakers. This is followed by Haitian-Creole, Arabic, Vietnamese, Hmong, Chinese, and Korean…

  13. 78 FR 4305 - Common Crop Insurance Regulations; Florida Citrus Fruit Crop Insurance Provisions; Correction

    Science.gov (United States)

    2013-01-22

    ... Fruit. DATES: Effective Date: January 22, 2013. FOR FURTHER INFORMATION CONTACT: Tim Hoffmann, Director... Corporation 7 CFR Part 457 RIN 0563-AC39 Common Crop Insurance Regulations; Florida Citrus Fruit Crop... corrections revised the Florida Citrus Fruit Crop Insurance Provisions that published on Friday, December...

  14. 33 CFR 165.767 - Security Zone; Manbirtee Key, Port of Manatee, Florida.

    Science.gov (United States)

    2010-07-01

    ..., Port of Manatee, Florida. 165.767 Section 165.767 Navigation and Navigable Waters COAST GUARD... § 165.767 Security Zone; Manbirtee Key, Port of Manatee, Florida. (a) Regulated area. The following area... extending 500 yards from the island's shoreline, in all directions, not to include the Port Manatee...

  15. 48 CFR 1852.247-71 - Protection of the Florida manatee.

    Science.gov (United States)

    2010-10-01

    ... manatee. 1852.247-71 Section 1852.247-71 Federal Acquisition Regulations System NATIONAL AERONAUTICS AND... and Clauses 1852.247-71 Protection of the Florida manatee. As prescribed in 1847.7001, insert the following clause: Protection of the Florida Manatee (MAR 1989) (a) Pursuant to the Endangered Species Act...

  16. Trends in Technology Planning and Funding in Florida K-12 Public Schools

    Science.gov (United States)

    Ritzhaupt, Albert Dieter; Hohlfeld, Tina N; Barron, Ann E.; Kemker, Kate

    2008-01-01

    This empirical research investigates trends in technology planning and funding in Florida's K-12 public schools between the 2003-04 and 2005-06 academic years. Survey items that focused on funding and planning issues on Florida's statewide school technology integration survey were analyzed using logistic models. Results indicate a significant…

  17. Florida Rising: An Assessment of Public Universities in the Sunshine State

    Science.gov (United States)

    Poliakoff, Michael; Alacbay, Armand

    2013-01-01

    The State University System of Florida has in recent years faced major budgetary challenges, remarkable for the size of its reductions in state funding, even when compared to the large cuts seen in so many states struck by the recession of 2008. What is more surprising in the world of higher education, however, is the progress that Florida's…

  18. Economic impact of tropical soda apple (Solanum viarum) on Florida cattle production

    Science.gov (United States)

    A written survey administered to 3,500 Florida cattle producers in 2006 documented tropical soda apple, as the most common pasture weed across the state of Florida. Over 80% of the survey respondents reported tropical soda apple on their ranches, and over 65% declared the plant to be a major proble...

  19. 76 FR 28130 - Coastal Bank, Cocoa Beach, Florida; Notice of Appointment of Receiver

    Science.gov (United States)

    2011-05-13

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF THE TREASURY Office of Thrift Supervision Coastal Bank, Cocoa Beach, Florida; Notice of Appointment of Receiver Notice... sole Receiver for Coastal Bank, Cocoa Beach, Florida, (OTS No. 15445) on May 6, 2011. Dated: May...

  20. Florida "State" Migrant Health Project. Third Annual Progress Report, 1965-1966.

    Science.gov (United States)

    Florida State Board of Health, Jacksonville.

    The Third Annual Report on the Florida Migrant Health Project covered migrant health activities engaged in by 14 counties for the period May 1, 1965, through April 30, 1966. The project was designed to develop a basic statewide program of health services for migrant farm workers and their dependents in Florida. Three of the 12 objectives included…

  1. Impacts of Hurricanes Frances and Jeanne on Two Nourished Beaches along the Southeast Florida Coast

    NARCIS (Netherlands)

    Benedet, L.; Campbell, T.; Finkl, C.W.; Stive, M.J.F.; Spadoni, R.

    2005-01-01

    Site inspections and beacli profile surveys of nourislied beaclies in the city of Boca Raton, and Town of Palm Beach, Florida show that the nourished beaches protected the shore from hurricane impacts in 2004. Striking the southeast coast of Florida within 20 days of each other. Hurricane Frances (S

  2. Genetic structure of Florida green turtle rookeries as indicated by mitochondrial DNA control region sequences

    Science.gov (United States)

    Shamblin, Brian M.; Bagley, Dean A.; Ehrhart, Llewellyn M.; Desjardin, Nicole A.; Martin, R. Erik; Hart, Kristen M.; Naro-Maciel, Eugenia; Rusenko, Kirt; Stiner, John C.; Sobel, Debra; Johnson, Chris; Wilmers, Thomas; Wright, Laura J.; Nairn, Campbell J.

    2014-01-01

    Green turtle (Chelonia mydas) nesting has increased dramatically in Florida over the past two decades, ranking the Florida nesting aggregation among the largest in the Greater Caribbean region. Individual beaches that comprise several hundred kilometers of Florida’s east coast and Keys support tens to thousands of nests annually. These beaches encompass natural to highly developed habitats, and the degree of demographic partitioning among rookeries was previously unresolved. We characterized the genetic structure of ten Florida rookeries from Cape Canaveral to the Dry Tortugas through analysis of 817 base pair mitochondrial DNA (mtDNA) control region sequences from 485 nesting turtles. Two common haplotypes, CM-A1.1 and CM-A3.1, accounted for 87 % of samples, and the haplotype frequencies were strongly partitioned by latitude along Florida’s Atlantic coast. Most genetic structure occurred between rookeries on either side of an apparent genetic break in the vicinity of the St. Lucie Inlet that separates Hutchinson Island and Jupiter Island, representing the finest scale at which mtDNA structure has been documented in marine turtle rookeries. Florida and Caribbean scale analyses of population structure support recognition of at least two management units: central eastern Florida and southern Florida. More thorough sampling and deeper sequencing are necessary to better characterize connectivity among Florida green turtle rookeries as well as between the Florida nesting aggregation and others in the Greater Caribbean region.

  3. Preminary Performance Evaluation of a Near Zero Energy Home in Gainesville, Florida

    Energy Technology Data Exchange (ETDEWEB)

    Parker, Danny [Florida Solar Energy Center, Cocoa, FL (United States); Sherwin, John [Florida Solar Energy Center, Cocoa, FL (United States); Hoak, David [Florida Solar Energy Center, Cocoa, FL (United States); Chandra, Subrao [Florida Solar Energy Center, Cocoa, FL (United States); Martin, Eric [Florida Solar Energy Center, Cocoa, FL (United States)

    2009-02-20

    This case study is a summary on a near zero energy home that was built in Gainesville, FL as a result of collaboration between the Florida Solar Enegery Center and the Florida H.E.R.O., an innovative developer and builder.

  4. 40 CFR 81.49 - Southeast Florida Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Quality Control Region. 81.49 Section 81.49 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Air Quality Control Regions § 81.49 Southeast Florida Intrastate Air Quality Control Region. The Southeast Florida Intrastate Air Quality Control Region is redesignated to consist of the territorial...

  5. 40 CFR 81.97 - Southwest Florida Intrastate Air Quality Control Region.

    Science.gov (United States)

    2010-07-01

    ... Quality Control Region. 81.97 Section 81.97 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY... Air Quality Control Regions § 81.97 Southwest Florida Intrastate Air Quality Control Region. The Southwest Florida Intrastate Air Quality Control Region consists of the territorial area encompassed by...

  6. Siting Study Framework and Survey Methodology for Marine and Hydrokinetic Energy Projects in Offshore Southeast Florida

    Energy Technology Data Exchange (ETDEWEB)

    Vinick, Charles [Dehlsen Associates, Inc., Santa Barbara, CA (United States)

    2011-09-26

    Presentation from the 2011 Water Peer Review in which principal investigator discusses project progess to Investigate areas offshore southeast Florida that appeared most suitable for siting of marine and hydrokinetic energy conversion facilities that may be proposed in the Atlantic Ocean offshore of southeast Florida.

  7. 75 FR 13524 - Northern Natural Gas Company, Southern Natural Gas Company, Florida Gas Transmission Company, LLC...

    Science.gov (United States)

    2010-03-22

    ... Energy Regulatory Commission Northern Natural Gas Company, Southern Natural Gas Company, Florida Gas... of Application March 16, 2010. Take notice that on March 5, 2010, Northern Natural Gas Company... other owners, Southern Natural Gas Company, Florida Gas Transmission Company, LLC, Transcontinental...

  8. Oil characterization and distribution in shoreline sediments of Pensacola Bay, Florida following the Deepwater Horizon spill

    Science.gov (United States)

    Barrier islands of Northwest Florida were heavily oiled during the Deepwater Horizon spill, but less is known about the impacts to the shorelines of the associated estuaries. Shoreline sediment oiling was investigated at 18 sites within the Pensacola Bay, Florida system prior to...

  9. 77 FR 25474 - Cross-Media Electronic Reporting: Authorized Program Revision Approval, State of Florida

    Science.gov (United States)

    2012-04-30

    ... Programs; and Part 272--Approved State Hazardous Waste Management Programs. FDEP was notified of EPA's... AGENCY Cross-Media Electronic Reporting: Authorized Program Revision Approval, State of Florida AGENCY... State of Florida's request ] to revise/modify certain of its EPA-authorized programs to allow...

  10. 78 FR 68997 - Approval and Promulgation of Implementation Plans; Florida; Approval of Revision to the State...

    Science.gov (United States)

    2013-11-18

    ... Organic Fiber Production.'' EPA has determined that this provision was erroneously incorporated into the....) entitled ``Synthetic Organic Fiber Production'' was inadvertently incorporated into the Florida SIP on June... synthetic organic fiber production facilities in northwest Florida. The rule was only concerned...

  11. 33 CFR 165.T08-290 - Safety Zone; Gulf of Mexico-Johns Pass, Florida.

    Science.gov (United States)

    2010-07-01

    ... Pass, Florida. 165.T08-290 Section 165.T08-290 Navigation and Navigable Waters COAST GUARD, DEPARTMENT... § 165.T08-290 Safety Zone; Gulf of Mexico—Johns Pass, Florida. (a) Regulated area. The Coast Guard is...'s Pass Bridge, that includes all the waters from surface to bottom, within a 100-yard radius of...

  12. 77 FR 71688 - Avocados Grown in South Florida; Decreased Assessment Rate

    Science.gov (United States)

    2012-12-04

    ... Agricultural Marketing Service 7 CFR Part 915 Avocados Grown in South Florida; Decreased Assessment Rate AGENCY... the assessment rate established for the Avocado Administrative Committee (Committee) for the 2012-13 and subsequent fiscal periods from $0.37 to $0.25 per 55-pound bushel container of Florida...

  13. Master's Degrees in the State University System of Florida. Information Brief. Volume 6, Issue 1

    Science.gov (United States)

    Florida Board of Governors, State University System, 2008

    2008-01-01

    A master's degree is a postbaccalaureate academic degree awarded after one to three years of full-time academic study. The State University System (SUS) of Florida offers master's degree programs in a variety of disciplines and at all universities, with the exception of New College. Students travel to Florida from throughout the world to earn…

  14. Tospoviruses and Thrips and Integrated Resistance Management Strategies in Pepper in Florida

    Science.gov (United States)

    Florida ranks second in the production and value of bell pepper in the U.S. In 2015, Florida produced over one-half billion pounds of bell pepper on over 12,000 acres, valued at over 220 million dollars. In recent years, several invasive species of thrips and thrips-vectored tospoviruses have beco...

  15. RECOMMENDED FOUNDATION FILL MATERIALS CONSTRUCTION STANDARD OF THE FLORIDA RADON RESEARCH PROGRAM

    Science.gov (United States)

    The report summarizes the technical basis for a recommended foundation fill materials standard for new construction houses in Florida. he radon-control construction standard was developed by the Florida Radon Research Program (FRRP). ill material standards are formulated for: (1)...

  16. 75 FR 17463 - Key West Bank, Key West, Florida; Notice of Appointment of Receiver

    Science.gov (United States)

    2010-04-06

    ... From the Federal Register Online via the Government Publishing Office ] DEPARTMENT OF THE TREASURY Office of Thrift Supervision Key West Bank, Key West, Florida; Notice of Appointment of Receiver Notice... sole Receiver for Key West Bank, Key West, Florida, (OTS No. 14929) on March 26, 2010. Dated: March...

  17. If Animals Could Talk: Bald Eagle, Bear, Florida Panther, Gopher Tortoise, Indigo Snake, Manatee, Otter, Raccoon.

    Science.gov (United States)

    Pinellas County District School Board, Clearwater, FL.

    In this series of booklets, eight Florida animals describe their appearance, habitats, food, behavior, and relationships with humans. Each entry is written for elementary students from the animal's point of view and includes a bibliography. Contained are the life stories of the bald eagle, black bear, Florida panther, gopher tortoise, Eastern…

  18. Genetic relatedness of dengue viruses in Key West, Florida, USA, 2009-2010.

    Science.gov (United States)

    Muñoz-Jordán, Jorge L; Santiago, Gilberto A; Margolis, Harold; Stark, Lillian

    2013-04-01

    Sequencing of dengue virus type 1 (DENV-1) strains isolated in Key West/Monroe County, Florida, indicate endemic transmission for >2 years of a distinct and predominant sublineage of the American-African genotype. DENV-1 strains isolated elsewhere in Florida grouped within a separate Central American lineage. Findings indicate endemic transmission of DENV into the continental United States.

  19. President Ilves külas Tampa, Floridas / Jüri Linask

    Index Scriptorium Estoniae

    Linask, Jüri

    2008-01-01

    President Toomas Hendrik Ilvese esinemisest Lõuna-Florida Tampa ülikoolis (University of South Florida School of Business). Riigipea rääkis Eesti majandustõusust ja tehnoloogilisest arengust, NATO küberkaitsekeskuse asutamisest Tallinnas ning vastas kuulajate küsimustele. Vabariigi President töövisiidil Ameerika Ühendriikides 17.-23.04.2008

  20. Utilizing Depth of Colonization of Seagrasses to Develop Numeric Water Quality Criteria for Florida Estuaries

    Science.gov (United States)

    US EPA is working with state and local partners in Florida to develop numeric water quality criteria to protect estuaries from nutrient pollution. Similar to other nutrient management programs in Florida, EPA is considering status of seagrass habitats as an indicator of biologic...

  1. Genome Sequence of Euphorbia mosaic virus from Passionfruit and Euphorbia heterophylla in Florida

    Science.gov (United States)

    Londoño, M. A.; Cohen, A. L.; Padilla-Rodriguez, M.; Rosario, K.; Breitbart, M.

    2017-01-01

    ABSTRACT Euphorbia mosaic virus (EuMV) was found in a symptomatic passionfruit (Passiflora edulis) plant from Homestead, Florida, USA, as well as in the symptomatic weed Euphorbia heterophylla. This is the first identification of EuMV in Florida and the United States and the first report of a natural infection of passionfruit by EuMV. PMID:28254981

  2. Water withdrawals, use, discharge, and trends in Florida, 1995

    Science.gov (United States)

    Marella, R.L.

    1999-01-01

    In 1995, the total amount of water withdrawn in Florida was nearly 18,200 million gallons per day (Mgal/d), of which 60 percent was saline and 40 percent was freshwater. Ground water accounted for 60 percent of freshwater withdrawals and surface water accounted for the remaining 40 percent. Ninety-three percent of the 14.15 million people in Florida relied on ground water for their drinking water needs in 1995. Almost all (99.9 percent) saline water withdrawals were from surface water. Public supply accounted for 43 percent of ground water withdrawn in 1995, followed by agricultural self-supplied (35 percent), commercial-industrial self-supplied (including mining) (10 percent), domestic self-supplied (7 percent), recreational irrigation (4.5 percent), and power generation (0.5 percent). Agricultural self-supplied accounted for 60 percent of fresh surface water withdrawn in 1995, followed by power generation (21 percent), commercial-industrial self-supplied (9 percent), public supply (7 percent), and recreational irrigation (3 percent). Almost all of saline water withdrawn was used for power generation. The largest amount of freshwater was withdrawn in Palm Beach County and the largest amount of saline water was withdrawn in Hillsborough County. Significant withdrawals (more than 200 Mgal/d) of fresh ground water occurred in Dade, Broward, Polk, Orange, and Palm Beach Counties. Significant withdrawals (more than 200 Mgal/d) of fresh surface water occurred in Palm Beach, Hendry, and St. Lucie Counties. The South Florida Water Management District accounted for the largest amount of freshwater withdrawn (nearly 50 percent). About 57 percent of the total ground water withdrawn was from the Floridan aquifer system; 20 percent was from the Biscayne aquifer. Most of the surface water used in Florida was from managed and maintained canal systems or large water bodies. Major sources of fresh surface water include the Caloosahatchee River, Deer Point Lake, Hillsborough River

  3. Water withdrawals, use, discharge, and trends in Florida, 2000

    Science.gov (United States)

    Marella, Richard L.

    2004-01-01

    In 2000, the estimated amount of water withdrawn in Florida was 20,148 million gallons per day (Mgal/d), of which 59 percent was saline and 41 percent was fresh. Ground water accounted for 62 percent of freshwater withdrawals and surface water accounted for the remaining 38 percent. Ninety-two percent of the 15.98 million people in Florida relied on ground water for their drinking water needs in 2000. Almost all of the saline water withdrawals (99.9 percent) were from surface water. Public supply accounted for 43 percent of ground water withdrawn in 2000, followed by agricultural self-supplied (39 percent), commercial-industrial self-supplied (including mining) (8.5 percent), recreational irrigation (4.5 percent), domestic self-supplied (4 percent), and power generation (1 percent). Agricultural self-supplied accounted for 62 percent of fresh surface water withdrawn in 2000, followed by power generation (20 percent), public supply (8 percent), recreational irrigation (6 percent), and commercial-industrial self-supplied (4 percent). Almost all of saline water withdrawn was used for power generation. The largest amount of freshwater was withdrawn in Palm Beach County and the largest amount of saline water was withdrawn in Hillsborough County. Significant withdrawals (more than 200 Mgal/d) of fresh ground water occurred in Miami-Dade, Polk, Orange, Palm Beach, Broward, and Collier Counties. Significant withdrawals (more than 200 Mgal/d) of fresh surface water occurred in Palm Beach, Hendry, and Escambia Counties. The South Florida Water Management District accounted for the largest amount of freshwater withdrawn (49 percent). About 62 percent of the total ground water withdrawn was from the Floridan aquifer system; 17 percent was from the Biscayne aquifer. Most of the surface water used in Florida was from managed and maintained canal systems or large water bodies. Major sources of fresh surface water include the Caloosahatchee River, Deer Point Lake, Hillsborough

  4. Geospatial characteristics of Florida's coastal and offshore environments: Administrative and political boundaries and offshore sand resources

    Science.gov (United States)

    Demopoulos, Amanda W.J.; Foster, Ann M.; Jones, Michal L.; Gualtieri, Daniel J.

    2011-01-01

    The Geospatial Characteristics Geopdf of Florida's Coastal and Offshore Environments is a comprehensive collection of geospatial data describing the political and natural resources of Florida. This interactive map provides spatial information on bathymetry, sand resources, military areas, marine protected areas, cultural resources, locations of submerged cables, and shipping routes. The map should be useful to coastal resource managers and others interested in the administrative and political boundaries of Florida's coastal and offshore region. In particular, as oil and gas explorations continue to expand, the map may be used to explore information regarding sensitive areas and resources in the State of Florida. Users of this geospatial database will find that they have access to synthesized information in a variety of scientific disciplines concerning Florida's coastal zone. This powerful tool provides a one-stop assembly of data that can be tailored to fit the needs of many natural resource managers.

  5. Comparison of Airborne Lidar and Multibeam Bathymetric Data in the Florida Reef Tract Along Broward County

    Science.gov (United States)

    Morton, N. E.; Burd, J. J.

    2003-12-01

    Although large, well-known concentrations of corals are found in deeper waters off Florida's eastern seaboard, most mapping of Florida's coral resources addresses the relatively shallow waters of the Florida Keys. To date, technological limitations precluded mapping corals in these deeper waters. Satellite imaging systems and natural color aerial photography, two mapping mainstays, are generally only effective in Florida waters shallower than 20 meters. Conservation of the northern portion of the Florida reef tract, which parallels the Atlantic coast in Miami-Dade, Broward, and Palm Beach counties, has been hampered by minimal or nonexistent coordinated management, monitoring, and mapping activities. In November 2000, the Simrad EM3000 multibeam system was used to collect data south of Port Everglades. Additionally, the Broward County shore protection project conducted a Laser Airborne Depth Sounder (LADS) survey in 2001. Wavelet analyses performed on overlapping transects of the two data sets compare the accuracy of reef bathymetry and complexity captured in the two data collection projects.

  6. Landscape and climate science and scenarios for Florida

    Science.gov (United States)

    Terando, Adam; Traxler, Steve; Collazo, Jaime

    2014-01-01

    The Peninsular Florida Landscape Conservation Cooperative (PFLCC) is part of a network of 22 Landscape Conservation Cooperatives (LCCs) that extend from Alaska to the Caribbean. LCCs are regional-applied conservation-science partnerships among Federal agencies, regional organizations, States, tribes, nongovernmental organizations (NGOs), private stakeholders, universities, and other entities within a geographic area. The goal of these conservation-science partnerships is to help inform managers and decision makers at a landscape scale to further the principles of adaptive management and strategic habitat conservation. A major focus for LCCs is to help conservation managers and decision makers respond to large-scale ecosystem and habitat stressors, such as climate change, habitat fragmentation, invasive species, and water scarcity. The purpose of the PFLCC is to facilitate planning, design, and implementation of conservation strategies for fish and wildlife species at the landscape level using the adaptive management framework of strategic habitat conservation—integrating planning, design, delivery, and evaluation. Florida faces a set of unique challenges when responding to regional and global stressors because of its unique ecosystems and assemblages of species, its geographic location at the crossroads of temperate and tropical climates, and its exposure to both rapid urbanization and rising sea levels as the climate warms. In response to these challenges, several landscape-scale science projects were initiated with the goal of informing decision makers about how potential changes in climate and the built environment could impact habitats and ecosystems of concern in Florida and the Southeast United States. In June 2012, the PFLCC, North Carolina State University, convened a workshop at the U.S. Geological Survey (USGS) Coastal and Marine Science Center in St. Petersburg to assess the results of these integrated assessments and to foster an open dialogue about

  7. Disney characters greet prime ASTP crewmen to Florida's Disney World

    Science.gov (United States)

    1975-01-01

    Two Walt Disney comic cartoon characters, Donald Duck and Pluto, were on hand to greet a group of Apollo-Soyuz Test Project crewmen on their arrival at Disney World near Orlando. From left, are interpreter K. S. Samofal, interpreter Nicholas Timacheff, Cosmonaut Vladimir A. Shatalov, Astronaut Vance D. Brand, Astronaut Donald K. Slayton, Cosmonaut Aleksey A. Leonov (squeezing Pluto's nose) and Astronaut Thomas P. Stafford. The astronauts and cosmonauts were in Florida for a three-day inspection tour of the Kennedy Space Center where they looked over ASTP launch facilities and flight hardware.

  8. Theoretical elementary particle research at the University of Florida

    Energy Technology Data Exchange (ETDEWEB)

    Field, R.D.; Ramond, P.M.; Sikivie, P.; Thorn, C.B. [Univ. of Florida, Gainesville, FL (United States)

    1996-12-01

    This is the annual progress report of the theoretical particle theory group at the University of Florida under DoE Grant DE-FG05-86ER40272. At present the group consists of four Full Professors (Field, Ramond, Thorn, Sikivie), two Associate Professors (Qiu, Woodard), and one Assistant Professor (Kennedy). In addition, the group has four postdoctoral research associates and three graduate students. The research of the group covers a broad range of topics in theoretical high energy physics including both theory and phenomenology. Included in this report is a summary of the last several years and an outline of the current research program.

  9. The Florida Image Slicer for Infrared Astrophysics and Cosmology

    Directory of Open Access Journals (Sweden)

    S. N. Raines

    2007-01-01

    Full Text Available We report on the design, manufacture, and scientific performance of the Florida Image Slicer for Infrared Astrophysics and Cosmol- ogy (FISICA, a fully cryogenic all-reective image slicing integral _eld unit (IFU for the FLAMINGOS near-infrared spectrograph (Elston et al. 2003. We find that FISICA is capable of delivering excellent scientific re- sults. It now operates as a turnkey instru- ment at the KPNO 4-m telescope via collab- oration with the instrument team, who can assist with the proposal preparation and ob- servations, as well as provide the data reduc- tion tools for integral field spectroscopy.

  10. Mycobacteriosis in two captive Florida manatees (Trichechus manatus latirostris).

    Science.gov (United States)

    Sato, Tsuneo; Shibuya, Hisashi; Ohba, Shigeo; Nojiri, Toshio; Shirai, Wataru

    2003-06-01

    Two male Florida manatees (Trichechus manatus latirostris) died at the marine aquarium in Inagi City, Tokyo, Japan. Acid-fast bacteria were demonstrated in tuberculoid nodules in the lungs from both manatees. Mycobacterium marinum and M. fortuitum were isolated from one manatee; M. marinum and M. kansasii were cultured from the second animal. This report confirms the pathogenicity and potentially fatal outcome of mycobacterial infection in manatees. In addition, the pathologic response to infection with these mycobacteria in manatees is similar to that associated with Mycobacterium spp. in other animals.

  11. Mercury methylation in periphyton of the Florida Everglades

    Science.gov (United States)

    Cleckner, L.B.; Gilmour, C.C.; Hurley, J.P.; Krabbenhoft, D.P.

    1999-01-01

    Trophic accumulation of mercury (Hg) in aquatic ecosystems is of global concern due to health effects associated with eating fish with elevated Hg levels. The methylated form of Hg bioaccumulates so it is important to understand how inorganic Hg is transformed to methylmercury in the environment. Here, a new site for Hg methylation, the periphyton communities that are prevalent in the Florida Everglades, is described. It is hypothesized that periphyton communities that support an active microbial sulfur cycle support Hg methylation. This new methylation site has implications for trophic transfer of methylmercury since periphyton can be the base of the food web in aquatic ecosystems.

  12. Connecting Florida Bay algal blooms to freshwater nutrient sources

    Science.gov (United States)

    Blakey, T.; Melesse, A. M.

    2013-12-01

    In this study, monthly water quality data collected in the Everglades by the Southeast Environmental Research Center (SERC) and the South Florida Water Management District (SFWMD) from 1991 to 2008 at 28 sampling stations distributed across Florida Bay was analyzed within the context of local geomorphology and seasonal wind and current regimes in order to evaluate the feasibility of the various purported nutrient sources for reoccurring algal blooms. The in situ chlorophyll-a (chl-a) measurements from the SERC dataset were evaluated as the indicator of algal biomass. Significant differences in average monthly chl-a concentrations at stations indicated a seasonality of algal blooms in the north central and west areas that is not evidenced in stations exhibiting low levels of chl-a throughout the typical year. Tukey's pairwise comparisons of monthly chl-a indicated, at the 95% confidence level, peak algal biomass occurs in October and November at the end of the wet season with minimums occurring between February and August depending on the location of the station. By month comparison of chl-a levels across stations suggest seasonal trends in the geographic focus and extent of blooms. Significant differences from Tukey's pairwise comparisons at the 95% confidence level showed stations to the west as having higher levels of chl-a in March through May with north central stations dominating from June to January. The month of February shows no significant difference in chl-a levels across this area. The results support hypotheses centering on a western source of nutrients that are delivered to the bay over the course of the rainy season. Mapping water quality sampling station locations on top of the bathymetry of Florida Bay illustrates the importance of considering coastal morphology in explaining trends in estuarine algal blooms. Coastal geomorphology along with seasonal changes in the direction of winds and magnitude of rains are demonstrated to be the predominant

  13. Weapons testing and endangered fish coexist in Florida

    Science.gov (United States)

    Jelks, Howard; Tate, Bill; Jordan, Frank

    2011-01-01

    Okaloosa darters (Etheostoma okaloosae) are small fish found only in a few streams in the Florida panhandle. This species has been listed since 1973 as endangered due to habitat alteration resulting from erosion, the potential competition from brown darters (E. edwini), and a limited geographic distribution. In recent years, however, Okaloosa darters have benefited from improved resource management and adaptive population monitoring techniques developed collaboratively by the U.S. Fish and Wildlife Service (FWS), U.S. Geological Survey (USGS), Loyola University New Orleans, and Eglin Air Force Base. As a result, the FWS reclassified the Okaloosa darter to the less critical category of threatened in March 2011.

  14. Recreational Fisheries in Biscayne National Park, Florida, 1976–19

    OpenAIRE

    Harper, Douglas E.; Bohnsack, James A.; Lockwood, Brian R.

    2000-01-01

    Recreational creel survey data from 28,923 intercepts collected from Biscayne National Park, Florida and surrounding waters were analyzed for January 1976 through July 1991, prior to disruptions caused by Hurricane Andrew in 1992. A total of 261,268 fish and shellfish representing 170 species or higher taxa were recorded. The average trip landed 9.03 fish and/or shellfish. Mean annual landings per angler were 4.77 fish/angler/trip (from 3.8 in 1991 to 5.83 in 1981) and dropped significantly...

  15. Water-management model in Florida from LANDSAT-1 data

    Science.gov (United States)

    Higer, A. L.; Cordes, E. H.; Coker, A. E.; Rogers, R. H.

    1975-01-01

    A prototype data acquisition and dissemination network and its effectiveness in improving and/or solving hydrologic problems in southern Florida are evaluated. The network utilized LANDSAT MSS imagery and in situ monitoring by LANDSAT-DCS. Results show water level and rain fall measurements were collected and disseminated in less than two hours, a significant improvement over conventional methods which took up to two months. Improved network performance has also aided the development of water budgets and water distribution to the people, funa, and flora in the area. Imagery from LANDSAT was also found to enhance the utility of ground measurements.

  16. North Florida is part of the stroke belt.

    OpenAIRE

    Siegel, P. Z.; Wolfe, L E; Wilcox, D.; Deeb, L C

    1992-01-01

    Florida is the only State in the southeastern United States that is not part of the "stroke belt." The National Heart, Lung, and Blood Institute (NHLBI), National Institutes of Health, defines the stroke belt as those States with an age-adjusted stroke mortality rate for the years 1979 to 1981 that is more than 10 percent above the national annual rate of 40.3 per 100,000 population. By reproducing at the county level the methods that were used at the State level to describe the stroke belt, ...

  17. Carbonate porosity versus depth: a predictable relation for south Florida.

    Science.gov (United States)

    Schmoker, J.W.; Halley, R.B.

    1982-01-01

    Examines the porosity of limestones and dolomites in the south Florida basin. Porosity data are derived from wire-line measurements which sample large volumes of rock, relative to petrographic methods, and can be examined at vertical scales approaching those of aquifers and hydrocarbon reservoirs. Investigation depths range from the surface to about 5500m. Curves of porosity versus depth, reflecting large-scale porosity-loss processes in the subsurface, are derived for a composite carbonate section and for carbonate strata of different ages and compositions.-from Authors

  18. Hurricane risk management and climate information gatekeeping in southeast Florida

    Science.gov (United States)

    Treuer, G.; Bolson, J.

    2013-12-01

    Tropical storms provide fresh water necessary for healthy economies and health ecosystems. Hurricanes, massive tropical storms, threaten catastrophic flooding and wind damage. Sea level rise exacerbates flooding risks from rain and storm surge for coastal communities. Climate change adaptation measures to manage this risk must be implemented locally, but actions at other levels of government and by neighboring communities impact the options available to local municipalities. When working on adaptation local decision makers must balance multiple types of risk: physical or scientifically described risks, legal risks, and political risks. Generating usable or actionable climate science is a goal of the academic climate community. To do this we need to expand our analysis to include types of risk that constrain the use of objective science. Integrating physical, legal, and political risks is difficult. Each requires specific expertise and uses unique language. An opportunity exists to study how local decision makers manage all three on a daily basis and how their risk management impacts climate resilience for communities and ecosystems. South Florida's particular vulnerabilities make it an excellent case study. Besides physical vulnerabilities (low elevation, intense coastal development, frequent hurricanes, compromised ecosystems) it also has unique legal and political challenges. Federal and state property rights protections create legal risks for government action that restricts land use to promote climate adaptation. Also, a lack of cases that deal with climate change creates uncertainty about the nature of these legal risks. Politically Florida is divided ideologically and geographically. The regions in the southeast which are most vulnerable are predominantly Hispanic and under-represented at the state level, where leadership on climate change is functionally nonexistent. It is conventional wisdom amongst water managers in Florida that little climate adaptation

  19. Genetic diversity and connectivity in the threatened staghorn coral (Acropora cervicornis in Florida.

    Directory of Open Access Journals (Sweden)

    Elizabeth M Hemond

    Full Text Available Over the past three decades, populations of the dominant shallow water Caribbean corals, Acropora cervicornis and A. palmata, have been devastated by white-band disease (WBD, resulting in the listing of both species as threatened under the U.S. Endangered Species Act. A key to conserving these threatened corals is understanding how their populations are genetically interconnected throughout the greater Caribbean. Genetic research has demonstrated that gene flow is regionally restricted across the Caribbean in both species. Yet, despite being an important site of coral reef research, little genetic data has been available for the Florida Acropora, especially for the staghorn coral, A. cervicornis. In this study, we present new mitochondrial DNA sequence data from 52 A. cervicornis individuals from 22 sites spread across the upper and lower Florida Keys, which suggest that Florida's A. cervicornis populations are highly genetically interconnected (F(ST = -0.081. Comparison between Florida and existing mtDNA data from six regional Caribbean populations indicates that Florida possesses high levels of standing genetic diversity (h = 0.824 relative to the rest of the greater Caribbean (h = 0.701+/-0.043. We find that the contemporary level of gene flow across the greater Caribbean, including Florida, is restricted (Phi(CT = 0.117, but evidence from shared haplotypes suggests the Western Caribbean has historically been a source of genetic variation for Florida. Despite the current patchiness of A. cervicornis in Florida, the relatively high genetic diversity and connectivity within Florida suggest that this population may have sufficient genetic variation to be viable and resilient to environmental perturbation and disease. Limited genetic exchange across regional populations of the greater Caribbean, including Florida, indicates that conservation efforts for A. cervicornis should focus on maintaining and managing populations locally rather than

  20. Genetic diversity and connectivity in the threatened staghorn coral (Acropora cervicornis) in Florida.

    Science.gov (United States)

    Hemond, Elizabeth M; Vollmer, Steven V

    2010-01-11

    Over the past three decades, populations of the dominant shallow water Caribbean corals, Acropora cervicornis and A. palmata, have been devastated by white-band disease (WBD), resulting in the listing of both species as threatened under the U.S. Endangered Species Act. A key to conserving these threatened corals is understanding how their populations are genetically interconnected throughout the greater Caribbean. Genetic research has demonstrated that gene flow is regionally restricted across the Caribbean in both species. Yet, despite being an important site of coral reef research, little genetic data has been available for the Florida Acropora, especially for the staghorn coral, A. cervicornis. In this study, we present new mitochondrial DNA sequence data from 52 A. cervicornis individuals from 22 sites spread across the upper and lower Florida Keys, which suggest that Florida's A. cervicornis populations are highly genetically interconnected (F(ST) = -0.081). Comparison between Florida and existing mtDNA data from six regional Caribbean populations indicates that Florida possesses high levels of standing genetic diversity (h = 0.824) relative to the rest of the greater Caribbean (h = 0.701+/-0.043). We find that the contemporary level of gene flow across the greater Caribbean, including Florida, is restricted (Phi(CT) = 0.117), but evidence from shared haplotypes suggests the Western Caribbean has historically been a source of genetic variation for Florida. Despite the current patchiness of A. cervicornis in Florida, the relatively high genetic diversity and connectivity within Florida suggest that this population may have sufficient genetic variation to be viable and resilient to environmental perturbation and disease. Limited genetic exchange across regional populations of the greater Caribbean, including Florida, indicates that conservation efforts for A. cervicornis should focus on maintaining and managing populations locally rather than relying on larval

  1. West Florida Shelf: A natural laboratory for the study of ocean acidificiation

    Science.gov (United States)

    Hallock, Pamela; Robbins, Lisa L.; Larson, Rebekka A.; Beck, Tanya; Schwing, Patrick; Martinez-Colon, Michael; Gooch, Brad

    2010-01-01

    Declining oceanic pH and carbonate-ion concentrations are well-known consequences of increased atmospheric and surface-ocean partial pressure of carbon dioxide (pCO2). The possible subject of shifts in seawater carbonate chemistry on biocalcification and survival rates of marine organisms provides questions amenable to both experimental and field study (Kleypas and Langdon, 2006). To date, limited quantitative data exist with which to formalize and test hypotheses regarding such impacts, particularly in continental-shelf settings. The continental shelves of Florida provide an ideal natural laboratory in which to test latitudinal (and temperature and depth) shifts in habitat ranges of calcifying organisms. Both the east and west Florida shelves extend from warm temperate to subtropical latitudes; additionally, the west Florida shelf has very little siliciclastic influx to mask the carbonate production. This study utilizes the natural laboratory of the west and southwest Florida shelf (fig 1.1) to examine the transition from foramol (predominately foraminifera and molluscan) carbonate sediments, characteristic of the west-central Florida shelf, to chlorozoan (algal and coral) sediments characteristic of the southwest Florida shelf. The west Florida shelf is a mixed siliciclastic carbonate ramp that to the south transitions to the carbonate-dominated southwest Florida shelf (Enos, 1977; Brooks and others, 2003). The west Florida shelf is a distally steepened carbonate ramp that is ~250 kilometers (km) wide (Read, 1985). It is covered by a veneer of unconsolidated sediment consisting of mainly biogenic carbonate and quartz in the near shore, with subordinate amounts of phosphate. The sediment-distribution pattern is largely a function of proximity to source, with physical processes playing a minor role in distribution. The carbonate sand-and-gravel fraction is produced by organisms within the depositional basin of the west Florida shelf (Brooks and others, 2003). The

  2. Subsurface storage of freshwater in south Florida; a prospectus

    Science.gov (United States)

    Merritt, M.L.; Meyer, F.W.; Sonntag, W.H.; Fitzpatrick, D.J.

    1983-01-01

    A method of increasing storage capacity for freshwater in south Florida is to use brackish artesian aquifers as reservoirs. In this way, water deficiencies occurring during the annual dry season can be offset by surplus water obtained during the wet season and injected underground. Most of south Florida is underlain by several deep, confined, carbonate waterbearing zones which might be suitable for freshwater storage. These zones are in the Avon Park, Ocala, Suwannee, Tampa, and Hawthorn Formations. Experimental freshwater injection systems have been operated at five locations with promising, but not fully definitive, results. A determination of the feasibility of freshwater injection at a selected site begins with an assessment of the local geologic suitability. Verification of feasibility, however, requires injection and recovery tests to be performed at the site. Recovery efficiency, a measure of the success of the operation, is the amount of potable water, expressed as a percentage of the volume injected, which can be recovered before its salinity, or the concentration of other chemical constituents present in the native aquifer water, increases to the point that the recovered water is no longer useable. (USGS)

  3. Nuclide migration and the environmental radiochemistry of Florida phosphogypsum.

    Science.gov (United States)

    Burnett, W C; Elzerman, A W

    2001-01-01

    Phosphogypsum, a waste by-product derived from the wet process production of phosphoric acid, represents one of the most serious problems facing the phosphate industry in Florida today. This by-product gypsum precipitates during the reaction of sulfuric acid with phosphate rock and is stored at a rate of about 40 million tons per year on several stacks in central and northern Florida. The main problem associated with this material concerns the relatively high levels of natural uranium-series radionuclides and other impurities which could have an impact on the environment and prevent its commercial use. We have studied the potential release of radionuclides from phosphogypsum by: (i) analysis of stack fluids, groundwaters, and soils associated with gypsum stacks; and (ii) geochemical modeling. Stack fluids were observed to be very high in dissolved uranium and 210Pb with only moderate concentrations of 226Ra. Underlying soils tend to be enriched in U and 210Pb indicating precipitation when acidic stack fluids enter a buffered environment. Modeling results showed significant increases in radionuclide complexes with sulfate and phosphate, resulting in relatively mobile uncharged or negatively charged solution species within the stacks with likely precipitation of multicomponent solids with increasing pH below the stack. Our evidence thus suggests that, while phosphogypsum stacks do contain significant quantities of dissolved radionuclides, removal mechanisms appear to prevent large-scale migration of radionuclides to the underlying aquifer.

  4. An epizootic of Florida manatees associated with a dinoflagellate bloom

    Science.gov (United States)

    O'Shea, T.J.; Rathbun, G.B.; Bonde, R.K.; Buergelt, C.D.; Odell, D.K.

    1991-01-01

    Over a 10-wk period in early 1982, 39 Florida manatees (Trichechus manatus latirostris) were found dead in the lower Caloosahatchee River and nearby waters of southwestern Florida. Two were killed by boats. The remainder showed no evidence of trauma. Lesions indicative of infectious agents were not identified, and bacteriological and contaminant residue findings were unremarkable. Nonspecific lesions of congestion and hemorrhage were identified in brain tissue. Numerous reports were also received of manatee morbidity. Some distressed manatees showed no biochemical lesions in clinical analyses of blood samples and recovered quickly. Timing of manatee illnesses coincided with fish and double-crested cormorant (Phalacrocorax auritus) mortality and morbidity. A widespread bloom of the dinoflagellate red tide organism (Gymnodinium breve) also coincided with these incidents. G. breve produces potent neurotoxins (brevetoxins). Circumstantial evidence links these events, and possible routes of exposure may include ingestion of filter-feeding ascidians. Ecological conditions that magnified the extent of the epizootic included an early dispersal of manatees into the area from a nearby winter aggregation site and unusually high salinities that facilitated the inshore spread of the red tide bloom. Management responses to future episodes of red tide in manatee areas are suggested.

  5. Florida seagrass habitat evaluation: A comparative survey for chemical quality

    Energy Technology Data Exchange (ETDEWEB)

    Lewis, Michael A. [U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, FL 32561 (United States)]. E-mail: lewis.michael@epa.gov; Dantin, Darrin D. [U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, FL 32561 (United States); Chancy, Cynthia A. [U.S. Environmental Protection Agency, Office of Research and Development, National Health and Environmental Effects Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, FL 32561 (United States); Abel, Kathryn C. [Department of Biological Sciences, University of West Florida, Pensacola, FL 32514 (United States); Lewis, Christopher G. [Department of Water and Soil Science, University of Florida, Gainesville, FL 32103 (United States)

    2007-03-15

    Contaminant concentrations were determined for media associated with 13 Florida seagrass beds. Concentrations of 10 trace metals were more commonly detected in surface water, sediment and two seagrass species than PAHs, pesticides and PCBs. Concentrations of copper and arsenic in surface water exceeded Florida aquatic life criteria more frequently than other trace elements. Total organic carbon, mercury, chromium, zinc, total chlordane, total PAHs, total PCBs, DDD and DDE were significantly greater in seagrass-rooted sediments than adjacent non-vegetated sediments. Total DDT, DDD, DDE, total chlordane, arsenic, copper and nickel exceeded proposed sediment quality guidelines at six of 13 grass beds. Pesticides, PAHs, and PCBs were below detection in seagrass tissues. Mercury, cadmium, nickel, lead and silver were detected in 50% or more of the tissues for Thalassia testudinum (turtle grass) and Halodule wrightii (shoal grass). Spatial, interspecific and tissue differences were usually an order of magnitude or less. - Some anthropogenic contaminants were prevalent in seagrass and their rooted sediments but the biological significance is unknown.

  6. Viral papillomatosis in Florida manatees (Trichechus manatus latirostris).

    Science.gov (United States)

    Bossart, Gregory D; Ewing, Ruth Y; Lowe, Mark; Sweat, Mark; Decker, Susan J; Walsh, Catherine J; Ghim, Shin-je; Jenson, A Bennett

    2002-02-01

    The Florida manatee (Trichechus manatus latirostris) is one of the most endangered marine mammals in American coastal waters. Naturally resistant to infectious disease, the manatee immune system appears highly developed to protect it against the harsh marine environment and the effects of human-related injury. In 1997, seven captive Florida manatees developed multiple, cutaneous, pedunculated papillomas over a period of 6 months. Approximately 3 years later, four of the seven manatees developed multiple, cutaneous, sessile papillomas topically and clinically distinct from the initial lesions, some of which are still present. Histologic, ultrastructural, and immunohistochemical features indicated that the two distinct phenotypic lesions were caused by papillomaviruses (PVs). Preliminary immunologic data correlated with daily clinical observations suggested that the manatees were immunologically suppressed and that the papillomas were caused by activation of latent PV infections and reinoculation from active infections. The emergence of PV-induced papillomas in captive manatees, the possibility of activation of latent infection or transmission of active infection to free-ranging manatees, and the underlying cause of immune suppression predisposing manatees to develop viral papillomatosis are serious concerns for the future management of this highly endangered species.

  7. Coral reef recovery in Florida and the Persian Gulf

    Science.gov (United States)

    Shinn, Eugene A.

    1976-01-01

    Long-term observations and study of coral reef destruction by hurricanes in the Florida Keys show, surprisingly, that although corals are devastated on a grand scale during storms, recovery is rapid. Recovery occurs because of the widespread scattering of live fragments, many of which become growth sites of new colonies. Reef recovery from death by chilling in the Persian Gulf was well under way when last observed, but it is not yet known if the recovery rate was as rapid as recovery from the storm destruction in Florida. Recovery from death by chilling requires settlement of transported coral larvae and a substrate suitable for larval attachment. Such resettlement is subject to the effects of currents, predators, pollution, and competition for substrate. A growth rate of 10 cm per year combined with geometrical progression of branch formation accounts for rapid recovery. Although calculated coral proliferation seems unusually high, it has been confirmed by serial underwater photographs spanning ten years. More precise measurements of growth and branching are needed, along with growth data for other common reef-building corals. Such data would be useful for predicting standing crop of a restocked or transplanted reef.

  8. Objective Lightning Probability Forecasts for East-Central Florida Airports

    Science.gov (United States)

    Crawford, Winfred C.

    2013-01-01

    The forecasters at the National Weather Service in Melbourne, FL, (NWS MLB) identified a need to make more accurate lightning forecasts to help alleviate delays due to thunderstorms in the vicinity of several commercial airports in central Florida at which they are responsible for issuing terminal aerodrome forecasts. Such forecasts would also provide safer ground operations around terminals, and would be of value to Center Weather Service Units serving air traffic controllers in Florida. To improve the forecast, the AMU was tasked to develop an objective lightning probability forecast tool for the airports using data from the National Lightning Detection Network (NLDN). The resulting forecast tool is similar to that developed by the AMU to support space launch operations at Kennedy Space Center (KSC) and Cape Canaveral Air Force Station (CCAFS) for use by the 45th Weather Squadron (45 WS) in previous tasks (Lambert and Wheeler 2005, Lambert 2007). The lightning probability forecasts are valid for the time periods and areas needed by the NWS MLB forecasters in the warm season months, defined in this task as May-September.

  9. Ecological correlates of invasion impact for Burmese pythons in Florida

    Science.gov (United States)

    Reed, R.N.; Willson, J.D.; Rodda, G.H.; Dorcas, M.E.

    2012-01-01

    An invasive population of Burmese pythons (Python molurus bivittatus) is established across several thousand square kilometers of southern Florida and appears to have caused precipitous population declines among several species of native mammals. Why has this giant snake had such great success as an invasive species when many established reptiles have failed to spread? We scored the Burmese python for each of 15 literature-based attributes relative to predefined comparison groups from a diverse range of taxa and provide a review of the natural history and ecology of Burmese pythons relevant to each attribute. We focused on attributes linked to spread and magnitude of impacts rather than establishment success. Our results suggest that attributes related to body size and generalism appeared to be particularly applicable to the Burmese python's success in Florida. The attributes with the highest scores were: high reproductive potential, low vulnerability to predation, large adult body size, large offspring size and high dietary breadth. However, attributes of ectotherms in general and pythons in particular (including predatory mode, energetic efficiency and social interactions) might have also contributed to invasion success. Although establishment risk assessments are an important initial step in prevention of new establishments, evaluating species in terms of their potential for spreading widely and negatively impacting ecosystems might become part of the means by which resource managers prioritize control efforts in environments with large numbers of introduced species.

  10. Marine biomass research in Florida. [Gracilaria tikvahiae, Ulva Lactuca

    Energy Technology Data Exchange (ETDEWEB)

    Ryther, J.H.

    1983-01-01

    The red alga Gracilaria tikvahiae may be grown outdoors year-round in central Florida with yields averaging 35.5 g dry weight/sq. m/day, greater than the most productive terrestrial plants. This occurs when the plants are grown in suspended culture, with vigorous aeration and an exchange of 25 or more culture volumes of enriched seawater per day. A culture system was designed in which Gracilaria, stocked at a density of 2 kg wet weight/sq.m, grows to double its biomass in one to two weeks; it is then harvested to its starting density, and anaerobically digested to methane. The biomass is soaked for 6 hours in the digester residue, storing enough nutrients for two week's growth in unenriched seawater. The methane is combusted for energy and the waste gas is fed to the culture to provide mixing of CO/sub 2/ eliminating the need for aeration and seawater exchange. The green alga Ulva lactuca, unlike Gracilaria, uses bicarbonate as a photosynthesis carbon source, and can be grown at high pH, with little or no free CO/sub 2/. It can therefore produce higher yields than Gracilaria in low water exchange conditions. It is also more efficiently converted to methane than is Gracilaria, but cannot tolerate Florida's summer temperatures so cannot be grown year-round. Attempts are being made to locate or produce through selective breeding, a high-temperature tolerant strain of Ulva. 21 references.

  11. The Application of GPR in Florida for Detecting Forensic Burials

    Energy Technology Data Exchange (ETDEWEB)

    S. K. Koppenjan; J. J. Schultz; S. Ono; H. Lee

    2003-01-01

    A study was performed at the University of Florida to measure ground penetrating radar(GPR) performance for detecting forensic burials. In controlled scenarios, 24 burials were constructed with pig cadavers. Two soils were utilized to represent two of the most common soil orders in Florida: an Entisol and an Ultisol. Graves were monitored on a monthly basis for time periods up to 21 months with grid data acquired with pulsed and swept-frequency GPR systems incorporating several different frequency antennas. A small subset of the graves was excavated to assess decomposition and relate to the GPR images during the test. The grave anomalies in the GPR depth profiles became less distinctive over time due to body decomposition and settling of the disturbed soil (backfill) as it compacted. Soil type was a major factor. Grave anomalies became more difficult to recognize over time for deep targets that were within clay. Forensic targets that were in sandy soil were recognized for the duration of this study. Time elapsed imagery will be presented to elucidate the changes, or lack thereof, of grave anomalies over the duration of this study. Further analysis was performed using Synthetic Aperture Radar (SAR) reconstruction of images in 2-D and 3-D.

  12. The Flood of June 2003 in Southwest Central Florida

    Science.gov (United States)

    Kane, Richard L.

    2003-01-01

    A series of severe thunderstorms brought heavy rainfall and flooding to southwest-central Florida during June 21-24, 2003. The storms and resultant precipitation were caused by a late-season stationary cool front (Canadian high pressure ridge) that combined with tropical moisture from the Gulf of Mexico to produce above-normal rainfall over already saturated ground (National Weather Service, written commun., 2003). Rainfall totals at U.S. Geological Survey (USGS) streamflow-gaging stations ranged from 9 to 17 inches and record flooding occurred in parts of Charlotte, De Soto, Hardee, Manatee, and Sarasota Counties, Florida (fig. 1). The floods caused $11.3 million in damage to public and private property, including damage or destruction of 119 homes (Binette and Saewitz, 2003). Western De Soto, western Hardee, Manatee, and Sarasota Counties are drained by the Horse Creek, Manatee River, and Myakka River systems. Water in these watersheds flows southwestward to Charlotte Harbor and the Gulf of Mexico (fig. 1).

  13. Sediment transport on Cape Sable, Everglades National Park, Florida

    Science.gov (United States)

    Zucker, Mark; Boudreau, Carrie

    2010-01-01

    The Cape Sable peninsula is located on the southwestern tip of the Florida peninsula within Everglades National Park (ENP). Lake Ingraham, the largest lake within Cape Sable, is now connected to the Gulf of Mexico and western Florida Bay by canals built in the early 1920's. Some of these canals breached a natural marl ridge located to the north of Lake Ingraham. These connections altered the landscape of this area allowing for the transport of sediments to and from Lake Ingraham. Saline intrusion into the formerly fresh interior marsh has impacted the local ecology. Earthen dams installed in the 1950's and 1960's in canals that breached the marl ridge have repeatedly failed. Sheet pile dams installed in the early 1990's subsequently failed resulting in the continued alteration of Lake Ingraham and the interior marsh. The Cape Sable Canals Dam Restoration Project, funded by ENP, proposes to restore the two failed dams in Lake Ingraham. The objective of this study was to collect discharge and water quality data over a series of tidal cycles and flow conditions to establish discharge and sediment surrogate relations prior to initiating the Cape Sable Canals Dam Restoration Project. A dry season synoptic sampling event was performed on April 27-30, 2009.

  14. Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida.

    Science.gov (United States)

    Merson, Samuel D; Ouwerkerk, Diane; Gulino, Lisa-Maree; Klieve, Athol; Bonde, Robert K; Burgess, Elizabeth A; Lanyon, Janet M

    2014-03-01

    The Florida manatee, Trichechus manatus latirostris, is a hindgut-fermenting herbivore. In winter, manatees migrate to warm water overwintering sites where they undergo dietary shifts and may suffer from cold-induced stress. Given these seasonally induced changes in diet, the present study aimed to examine variation in the hindgut bacterial communities of wild manatees overwintering at Crystal River, west Florida. Faeces were sampled from 36 manatees of known sex and body size in early winter when manatees were newly arrived and then in mid-winter and late winter when diet had probably changed and environmental stress may have increased. Concentrations of faecal cortisol metabolite, an indicator of a stress response, were measured by enzyme immunoassay. Using 454-pyrosequencing, 2027 bacterial operational taxonomic units were identified in manatee faeces following amplicon pyrosequencing of the 16S rRNA gene V3/V4 region. Classified sequences were assigned to eight previously described bacterial phyla; only 0.36% of sequences could not be classified to phylum level. Five core phyla were identified in all samples. The majority (96.8%) of sequences were classified as Firmicutes (77.3 ± 11.1% of total sequences) or Bacteroidetes (19.5 ± 10.6%). Alpha-diversity measures trended towards higher diversity of hindgut microbiota in manatees in mid-winter compared to early and late winter. Beta-diversity measures, analysed through PERMANOVA, also indicated significant differences in bacterial communities based on the season.

  15. Variation in the hindgut microbial communities of the Florida manatee, Trichechus manatus latirostris over winter in Crystal River, Florida

    Science.gov (United States)

    Merson, Samuel D.; Ouwerkerk, Diane; Gulino, Lisa-Maree; Klieve, Athol; Bonde, Robert K.; Burgess, Elizabeth A.; Lanyon, Janet M.

    2014-01-01

    The Florida manatee, Trichechus manatus latirostris, is a hindgut-fermenting herbivore. In winter, manatees migrate to warm water overwintering sites where they undergo dietary shifts and may suffer from cold-induced stress. Given these seasonally induced changes in diet, the present study aimed to examine variation in the hindgut bacterial communities of wild manatees overwintering at Crystal River, west Florida. Faeces were sampled from 36 manatees of known sex and body size in early winter when manatees were newly arrived and then in mid-winter and late winter when diet had probably changed and environmental stress may have increased. Concentrations of faecal cortisol metabolite, an indicator of a stress response, were measured by enzyme immunoassay. Using 454-pyrosequencing, 2027 bacterial operational taxonomic units were identified in manatee faeces following amplicon pyrosequencing of the 16S rRNA gene V3/V4 region. Classified sequences were assigned to eight previously described bacterial phyla; only 0.36% of sequences could not be classified to phylum level. Five core phyla were identified in all samples. The majority (96.8%) of sequences were classified as Firmicutes (77.3 ± 11.1% of total sequences) or Bacteroidetes (19.5 ± 10.6%). Alpha-diversity measures trended towards higher diversity of hindgut microbiota in manatees in mid-winter compared to early and late winter. Beta-diversity measures, analysed through permanova, also indicated significant differences in bacterial communities based on the season.

  16. Polymorphic microsatellite DNA markers for the Florida manatee (Trichechus manatus latirostris)

    Science.gov (United States)

    Pause, K.C.; Nourisson, C.; Clark, A.; Kellogg, M.E.; Bonde, R.K.; McGuire, P.M.

    2007-01-01

    Florida manatees (Trichechus manatus latirostris) are marine mammals that inhabit the coastal waters and rivers of the southeastern USA, primarily Florida. Previous studies have shown that Florida manatees have low mitochondrial DNA variability, suggesting that nuclear DNA loci are necessary for discriminatory analyses. Here we report 10 polymorphic microsatellite loci with an average of 4.2 alleles per locus, and average heterozygosity of 50.1%. These loci have been developed for use in population studies, parentage assignment, and individual identification. ?? 2007 Blackwell Publishing Ltd.

  17. GIS Assessment of Wind Energy Potential in California and Florida

    Science.gov (United States)

    Snow, R. K.; Snow, M. M.

    2008-05-01

    Energy efficiency coupled with renewable energy technologies can provide most of the U.S. carbon emissions reductions needed to contain atmospheric carbon concentrations at 450-500 parts per million, considered by many to be a tipping point in mitigating climate change. Among the leaders in the alternative energy sector is wind power, which is now one of the largest sources of new power generation in the U.S. creating jobs and revenue for rural communities while powering our economy with an emissions-free source of energy. In 2006, wind turbines capable of generating more than 2,400 megawatts of electricity were installed in the U.S. and by 2007 this number had risen to 3,000 megawatts. The U.S. generated 31 billion kilowatt-hours of wind power in 2007, which is enough electricity to power the equivalent of nearly 3 million average homes. It is estimated that generating the same amount of electricity would require burning 16 million tons of coal or 50 million barrels of oil. This study examines the wind power potential of sites near populated areas in Florida and California to determine the practicability of installing wind turbines at these locations. A GIS was developed in order to conduct a spatial analysis of these sites based on mean annual wind speed measured in meters per second and wind power density ratings measured in watts per square meter. The analysis indicates that coastal areas of Cocoa Beach, Key West, Hollywood, and West Palm Beach, respectively, possess the greatest potential for wind energy in Florida with mean annual wind speeds of 4.9 m/s and average wind power density ratings of 171 w/m2 peaking at Cocoa Beach followed by wind speeds of 4.64 m/s and wind power ratings of 115 w/m2 at Key West. California wind energy potential is even greater than that of Florida with Fairfield exhibiting mean annual wind speeds of 5.9 m/s and average wind power density ratings of 327 w/m2 followed by the Mojave and Palmdale areas with mean annual wind speeds of

  18. Satellite-Observed Black Water Events off Southwest Florida: Implications for Coral Reef Health in the Florida Keys National Marine Sanctuary

    Directory of Open Access Journals (Sweden)

    Brian Lapointe

    2013-01-01

    Full Text Available A “black water” event, as observed from satellites, occurred off southwest Florida in 2012. Satellite observations suggested that the event started in early January and ended in mid-April 2012. The black water patch formed off central west Florida and advected southward towards Florida Bay and the Florida Keys with the shelf circulation, which was confirmed by satellite-tracked surface drifter trajectories. Compared with a previous black water event in 2002, the 2012 event was weaker in terms of spatial and temporal coverage. An in situ survey indicated that the 2012 black water patch contained toxic K. brevis and had relatively low CDOM (colored dissolved organic matter and turbidity but high chlorophyll-a concentrations, while salinity was somewhat high compared with historical values. Further analysis revealed that the 2012 black water was formed by the K. brevis bloom initiated off central west Florida in late September 2011, while river runoff, Trichodesmium and possibly submarine groundwater discharge also played important roles in its formation. Black water patches can affect benthic coral reef communities by decreasing light availability at the bottom, and enhanced nutrient concentrations from black water patches support massive macroalgae growth that can overgrow coral reefs. It is thus important to continue the integrated observations where satellites provide synoptic and repeated observations of such adverse water quality events.

  19. Optical dating of the anastasia formation, northeastern florida, USA

    Science.gov (United States)

    Burdette, K.E.; Rink, J.W.; Means, G.H.; Portell, R.W.

    2009-01-01

    The single-aliquot regenerative-dose (SAR) procedure was used to obtain optically stimulated luminescence ages to determine the depositional age of the upper part of the Anastasia Formation. This unit, which crops out along the east coast of Florida, is one of the most culturally and economically important coquina deposits in North America. Rock samples from the upper three meters of exposure at three locations were collected. Additional materials for paleontological analysis were also taken. Based on our samples, the luminescence ages of the Anastasia Formation are well within marine isotope stage 5, which is supported by the results of Osmond et al. (1970) based on U/Th ages. The associated fossil assemblages support our luminescence age determinations. Associated fossils fall within the Rancholabrean North American Land Mammal Age (300 10 ka) and the fossil mollusk assemblage consists entirely of modern species.

  20. Cumulative Trauma Among Mayas Living in Southeast Florida.

    Science.gov (United States)

    Millender, Eugenia I; Lowe, John

    2016-01-04

    Mayas, having experienced genocide, exile, and severe poverty, are at high risk for the consequences of cumulative trauma that continually resurfaces through current fear of an uncertain future. Little is known about the mental health and alcohol use status of this population. This correlational study explored t/he relationship of cumulative trauma as it relates to social determinants of health (years in the United States, education, health insurance status, marital status, and employment), psychological health (depression symptoms), and health behaviors (alcohol use) of 102 Guatemalan Mayas living in Southeast Florida. The results of this study indicated that, as specific social determinants of health and cumulative trauma increased, depression symptoms (particularly among women) and the risk for harmful alcohol use (particularly among men) increased. Identifying risk factors at an early stage before serious disease or problems are manifest provides room for early screening leading to early identification, early treatment, and better outcomes.

  1. Mickey Mouse greets prime ASTP crewmen to Florida's Disney World

    Science.gov (United States)

    1975-01-01

    A space-suited Mickey Mouse character welcomes the prime crewmen of the Apollo Soyuz Test Project (ASTP) to Florida's Disney World near Orlando. The crewmen made a side-trip to Disney World during a three-day inspection tour of the Kennedy Space Center. Receiving the Disney World welcome are, left to right, Cosmonaut Valeriy N. Kubasov, engineer on the Soviet crew; Astronaut Donald K. Slayton, docking module pilot of the American crew; Astronaut Vance D. Brand, command module pilot of the American crew; Cosmonaut Aleksey A. Leonov, commander of the Soviet crew; Astronaut Thomas P. Stafford, commander of the American crew; and Cosmonaut Vladimir A. Shatalov, Chief of Cosmonaut Training for the U.S.S.R.

  2. Psychobiology and Neuroscience at the Florida State University: a history.

    Science.gov (United States)

    Rashotte, Michael E; Smith, James C

    2005-10-15

    In the 1950s, young faculty in Psychology and Physiology/Biology at the newly established Florida State University recognized common interests in the study of sensory systems. They spontaneously formed one of this country's earliest interdisciplinary research cohorts in the emerging field of "psychobiology". In the 1960s, this group established a formal graduate program in Psychobiology, acquired resources for building a new laboratory and for supporting pre- and post-doctoral students, and began the expansion of faculty and research focuses that continues to this day. In 1991, FSU's Psychobiology Program was re-branded as a Program in Neuroscience that awards a doctoral degree. It now encompasses faculty and students from four academic departments in the Colleges of Arts and Sciences, Human Sciences, and Medicine. This paper traces some main developments in our 50-year history of these research and training efforts.

  3. Integrated solid waste management of Palm Beach County, Florida

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-11-01

    The subject document reports the results of an in-depth investigation of the fiscal year 1992 cost of the Palm Beach County, Florida integrated municipal solid waste management system (IMSWMS), the energy consumed to operate the system, and the environmental performance requirements for each of the system`s waste-processing and disposal facilities. Actual data from records kept by participants is reported in this document. Every effort was made to minimize the use of assumptions, and no attempt is made to interpret the data reported. Analytical approaches are documented so that interested analysts may perform manipulation or further analysis of the data. As such, the report is a reference document for MSW management professionals who are interested in the actual costs and energy consumption for a one-year period, of an operating IMSWMS.

  4. Manatee use of power plant effluents in Brevard County, Florida

    Energy Technology Data Exchange (ETDEWEB)

    Shane, S.H.

    The relationship between manatees and power plants was investigated at 2 power plants on the Indian River in Brevard County, Florida from January 1978-February 1980. Manatee presence in the power plant effluent zones was correlated with cold air and water temperatures. When air temperatures were below 16 C most manatees in the country were found in the effluent zones. Manatees in the effluent zones move with the wind-blown warm water plume, demonstrating a sensitivity to small changes in water temperature. Some individuals were frequently resighted at 1 plant, while others moved between the 2 plants. Because industrial warm water sources are less reliable than natural warm water refuges, it is recommended that no new artificial warm water effluents be constructed north of the species' traditional winter range. 16 references, 3 figures, 1 table.

  5. Attempted eradication of Porphyrio porphyrio Linnaeus in the Florida Everglades

    Directory of Open Access Journals (Sweden)

    Dave EGGEMAN

    2011-01-01

    Full Text Available Porphyrio porphyrio (Fulica porphyrio Linnaeus was reported to the South Florida Water Management District in a Water Conservation Area and in constructed wetlands in the Everglades in 2006. A rapid assessment, including casual observations and surveys of land managers, indicated a limited number of P. porphyrio (~300 birds was present, and an eradication attempt was initiated. From 2006 – 2008, more than 3100 P. porphyrio were killed by shotgun from airboats during 73 hunts, suggesting the initial population assessment was severely underestimated. After removing nearly 1500 P. porphyrio in 2008, we concluded that eradication was not possible. Failure of this eradication attempt is attributed to P. porphyrio’s affinity with dense emergent vegetation, which greatly limited shooting effectiveness. Further, the failed eradication underscores the importance of a reporting network to improve early detection and the chance to eliminate naturalized or feral populations of non‐native species.

  6. Florida court asked to decide legality of limiting suits.

    Science.gov (United States)

    1996-06-14

    The state Supreme Court of Florida will rule on the legitimacy of a statute that restricts the ability of a plaintiff to sue for damages arising from HIV-contaminated blood transfusion. Under the malpractice law, patients have 4 years from the time of injury to file suit. In most HIV and AIDS cases, however, symptoms do not appear within the 4-year period. The Broward County Circuit Court recently dismissed a contaminated blood transfusion suit based on the expiration of the 4-year statute of repose. The Court of Appeals held the trial judge's application of the law correct, but found the statute uniquely unfair and harsh in HIV/AIDS cases. The State Supreme Court has been asked to rule on the issue.

  7. Aspectos de los sobrenatural en Pampas-La Florida (Chancay

    Directory of Open Access Journals (Sweden)

    Isabelle Lausent

    1984-12-01

    Full Text Available La Pacarina del pueblo de "San Salvador del Mundo" de Pampas (valle de Chancay es el cerro Mango. Las repetidas Extirpaciones de Idolatría que sufrieron "sus hijos", el mestizaje y el transcurso del tiempo han, poco a poco, hecho desaparecer los mitos que frataban del Mango. La tradición oral de Pampas que da mucha importercia al mundo de lo sobrenatural sigue, sin embargo, ofreciendo numerosos cuentos y leyendas mencionando este cerro ya no como pacarina sino como una mina fuente de riqueza y lugar de encanto.El análisis que sigue ha sido realizado a partir de Ics cuentos recopilados por Jorgc Osterling durante la Semana Santa del año de 1980 en el rnismo Pampas La Florida.

  8. Renegotiating property rights in the Florida golden crab fishery

    Directory of Open Access Journals (Sweden)

    Scott Crosson

    2013-08-01

    Full Text Available The golden crab (Chaceon fenneri supports a small, economically healthy fishery in south Florida. Crabbers in the fishery have successfully protected themselves against larger outside fishing interests in the past, and management has been stable for over fifteen years.  Why, then, did a portion of the fleet propose shifting to individual transferable quotas (ITQs?  Our findings suggest that proponents sought ITQ management because they believed it would further limit the ability of other crabbers to enter the fishery and act as a mechanism to legally preserve the informal and formal property rights that they have previously negotiated among themselves. Opponents believed that a shift to an ITQ regime would destroy those same property rights.  We explore the implications of these findings to a broader understanding of property rights and natural resource management institutions, noting that the currently existing system closely resembles a territorial use rights fishery (TURF.

  9. Instrumentation of urban hydrology monitoring sites in Southeast Florida

    Science.gov (United States)

    Hardee, Jack

    1979-01-01

    An instrumentation system developed and built in laboratories of the U.S. Geological Survey, Reston, Va., has been used since 1974 to collect synchronized rainfall, runoff, and water-quality data from urban basins. A number of field modifications were made to adapt the system to local hydrologic conditions and for collection of data on about 350 rainstorms at four sites in south Florida. The instrumentation system measures rainfall at three sites, records two pressure or water-level readings for flow computations, and collects and refrigerates up to 24 water samples and rainfall-quality samples. Rainfall and runoff data are recorded every 36 seconds on a six-channel analog recorder so that all variables are time-synchronous. Flow in the storm sewer is computed from pressure measurements in a U-shaped venturi-type constriction or, when the constriction is not used, from water levels. (Kosco-USGS)

  10. Photovoltaic test facility at Florida solar energy center

    Energy Technology Data Exchange (ETDEWEB)

    Atmanam, G.; Maytrott, C.; Wedekind, D.

    1984-05-01

    A photovoltaic flexible test facility has been developed at the Florida Solar Energy Center. The primary objective was to provide a test bed so that a variety of advanced technology subsystems (arrays and power conditioners) can be characterized and evaluated expeditiously in grid-interactive photovoltaic system operation. Also the systems' and subsystems' safety and reliability can be tested under imposed utility fault and extreme conditions. Such conditions include the utility outage, utility underand over-voltage and possible transient surges. The facility is designed to incorporate two complete parallel photovoltaic systems, one including the roof-mounted array and the other the tracking/adjustable array. The initial performance and test results are presented here along with a description of the facility.

  11. Water-management models in Florida from ERTS-1 data

    Science.gov (United States)

    Higer, A. L. (Principal Investigator); Rogers, R. H.; Coker, A. E.; Cordes, E. H.

    1975-01-01

    The author has identified the following significant results. The usefullness of ERTS 1 to improving the overall effectiveness of collecting and disseminating data was evaluated. ERTS MSS imagery and in situ monitoring by DCS were used to evaluate their separate and combined capabilities. Twenty data collection platforms were established in southern Florida. Water level and rainfall measurements were collected and disseminated to users in less than 2 hours, a significant improvement over conventional techniques requiring 2 months. ERTS imagery was found to significantly enhance the utility of ground measurements. Water stage was correlated with water surface areas from imagery in order to obtain water stage-volume relations. Imagery provided an economical basis for extrapolating water parameters from the point samples to unsampled data and provided a synoptic view of water mass boundaries that no amount of ground sampling or monitoring could provide.

  12. Winter mortality of common loons in Florida coastal waters

    Science.gov (United States)

    Forrester, Donald J.; Davidson, W.R.; Lange, R.E.; Stroud, R.K.; Alexander, L.L.; Franson, J.C.; Haseltine, S.D.; Littell, R.C.; Nesbitt, S.A.

    1997-01-01

    Diagnostic findings are presented for 434 common loons (Gavia imrner) found sick or dead on Florida beaches from 1970 through 1994, primarily during the months of December to April. The most commonly recognized problem was an emaciation syndrome (66%), followed by oiling (18%), aspergillosis (7%), trauma (5%) and miscellaneous disease entities (1%). The cause-of-death for 3% of the birds was not determined. Many of the carcasses examined (n = 173) were obtained during an epizootic which occurred from January to March of 1983 in which more than 13,000 loons were estimated to have died. An emaciation syndrome, characterized by severe atrophy of pectoral muscles, loss of body fat and hemorrhagic enteritis, was the primary finding in this epizootic. It was postulated to have a complex etiologic basis involving synergistic effects and energy costs of migration, molting and replacement of flight feathers, food resource changes, salt-loading, intestinal parasitism, environmental contaminants, and inclement weather.

  13. Storeria occipitomaculata obscura (Florida red-bellied snake)

    Science.gov (United States)

    Muse, Lindy J.; Glorioso, Brad M.; Eaglestone, Chandler A. R.

    2016-01-01

    USA: LOUISIANA: Vermilion Parish: Palmetto Island State Park (29.86335°N, 92.14848°W; WGS 84). 19 February 2016. Lindy J. Muse. Verified by Jeff Boundy. Florida Museum of Natural History (UF 177730, photo voucher). New parish record (Dundee and Rossman 1989. The Amphibians and Reptiles of Louisiana. Louisiana State University Press, Baton Rouge, Louisiana. 300 pp.). Storeria occipitomaculata obscura has not been documented in any of the coastal parishes of Louisiana (Boundy. 2006. Snakes of Louisiana. Louisiana Department of Wildlife & Fisheries, Baton Rouge, Louisiana. 40 pp.). However, this species can be difficult to find in southern Louisiana and other populations in coastal parishes may eventually be discovered. This adult individual (SVL = 292 mm; TL = 70 mm) was found under a log in a wet bottomland forest dominated by Dwarf Palmetto and Bald Cypress.

  14. Genetic variability in the Florida manatee (Trichechus manatus)

    Science.gov (United States)

    McClenaghan, Leroy R.; O'Shea, Thomas J.

    1988-01-01

    Tissue was obtained from 59 manatee (Trichechus manatus) carcasses salvaged from 20 counties in Florida. Allozyme phenotypes at 24 structural loci were determined by gel electrophoresis. Averages for the proportion of polymorphic loci and mean heterozygosity were 0.300 (range, 0.167-0.417) and 0.050 (range, 0.028-0.063), respectively. These estimates are equivalent to or higher than those generally reported for other species of marine mammals and do not support the hypothesis that body size and heterozygosity in mammals are related inversely. Among-region gene diversity accounted for only 4% of the total diversity. High rates of gene flow probably account for genetic homogeneity across regions. An F-statistic analysis revealed a general tendency toward excess homozygosity within regions. Management efforts to prevent future reductions in population size that would erode existing genic diversity should continue.

  15. Florida state information handbook: formerly utilized sites remedial action program

    Energy Technology Data Exchange (ETDEWEB)

    None

    1981-02-27

    This volume is one of a series produced under contract with DOE, Office of Nuclear Waste Management, by Politech Corporation to develop a legislative and regulatory data base to assist the FUSRAP management in addressing the institutional and socioeconomic issues involved in carrying out the Remedial Action Program. This Information Handbook series contains information about all relevant government agencies at the federal and state levels, the pertinent programs they administer, each affected state legislature, and current federal and state legislative and regulatory initiatives. This volume is a compilation of information about the State of Florida. It contains a description of the state executive branch structure; a summary of relevant state statutes and regulations; a description of the structure of the state legislature, identification of the officers and committee chairmen, and a summary of recent relevant legislative action; and the full text of relevant statutes and regulations.

  16. Reproductive ecology of the giant African snail in South Florida

    DEFF Research Database (Denmark)

    Roda, Amy; Nachman, Gøsta Støger; Weihman, Scott

    2016-01-01

    Giant African snail (Achatina fulica (Bowdich, 1822)), an important invasive snail, was recently found in South Florida, USA. An extensive eradication effort was initiated consisting of pesticide applications, debris removal and hand collections. We studied the reproduction capacity and population...... dynamics of snails collected from 22 populations for two years to help evaluate the likely success of the eradication program. A total of 23,890 snails, ranging from 25-131 mm, were measured, dissected and the number of eggs in each snail counted. Gravid snails ranged from 48-128 mm. Only 5% of snails had...... eggs, which were found year round. As the snails increased in size, they were more likely to include reproducing individuals. However, the percentage of gravid snails peaked when snails were approximately 90 mm. Although more prevalent, small (

  17. Data on selected deep wells in south Florida

    Science.gov (United States)

    Smith, C.A.; Lidz, Lauralee; Meyer, F.W.

    1982-01-01

    A data base of 870 deep wells was assembled for the south Florida part of the Southeastern Limestone Regional Aquifer Study. The data in this report are for wells in Broward, Collier , Dade, Hendry, Lee, Martin, Monroe, Palm Beach, and St. Lucie Counties. Data are compiled for water wells, oil test wells, injection wells, and monitor wells. These data consist of: (1) well identification and geographic location; (2) well construction and site use; (3) logs available (geologic and geophysical); and (4) selected water-quality parameters. The well locations are shown, by counties, on maps. The number of wells in each county are: Broward, 6; Collier, 237; Dade, 61; Hendry, 130; Lee, 226; Martin, 91; Monroe, 34; Palm Beach, 33; and St. Lucie, 52. Data are stored in the National Water Data Storage and Retrieval system (WATSTORE) in the following files: header, water-quality, and ground-water site inventory (GWSI). (USGS)

  18. Presence of arbuscular mycorrhizal fungi in South Florida native plants.

    Science.gov (United States)

    Fisher, Jack B; Jayachandran, K

    2005-11-01

    The roots of 27 species of South Florida plants in 15 families (including one cycad, six palms, one Smilax, and 19 dicotyledons) native to pine rockland and tropical hardwood hammock communities were examined for arbuscular mycorrhizal fungi (AMF). These plants grow in the biologically diverse but endangered Greater Everglades habitat. Roots from field-grown and potted plants were cleared and stained. All 27 species had AMF and include 14 species having an endangered or threatened status. The Paris-type colonization occurred in two species in the families Annonaceae and Smilacaceae. The Arum-type occurred in 22 species in the families Anacardiaceae, Arecaceae (Palmae), Boraginaceae, Cactaceae (questionable), Euphorbiaceae, Fabaceae, Lauraceae, Melastomataceae, Polygalaceae, Rubiaceae, Simaroubaceae, Ulmaceae, and Zamiaceae. Three species in the families Fabaceae, Lauraceae, and Simaroubaceae had a mix of Paris- and Arum-types. The results have implications for the restoration of these endangered plant communities in the Everglades.

  19. ATM Coastal Topography-Florida 2001: Western Panhandle

    Science.gov (United States)

    Yates, Xan; Nayegandhi, Amar; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Klipp, Emily S.; Wright, C. Wayne

    2009-01-01

    These remotely sensed, geographically referenced elevation measurements of Lidar-derived first surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of the western Florida panhandle coastline, acquired October 2-4 and 7-10, 2001. The datasets are made available for use as a management tool to research scientists and natural resource managers. An innovative scanning Lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning Lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of Lidar data in an interactive or batch mode. Modules for presurvey flight line definition, flight path plotting, Lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is routinely used

  20. ATM Coastal Topography-Florida 2001: Eastern Panhandle

    Science.gov (United States)

    Yates, Xan; Nayegandhi, Amar; Brock, John C.; Sallenger, A.H.; Bonisteel, Jamie M.; Klipp, Emily S.; Wright, C. Wayne

    2009-01-01

    These remotely sensed, geographically referenced elevation measurements of Lidar-derived first surface (FS) topography were produced collaboratively by the U.S. Geological Survey (USGS), Florida Integrated Science Center (FISC), St. Petersburg, FL, and the National Aeronautics and Space Administration (NASA), Wallops Flight Facility, VA. This project provides highly detailed and accurate datasets of the eastern Florida panhandle coastline, acquired October 2, 2001. The datasets are made available for use as a management tool to research scientists and natural resource managers. An innovative scanning Lidar instrument originally developed by NASA, and known as the Airborne Topographic Mapper (ATM), was used during data acquisition. The ATM system is a scanning Lidar system that measures high-resolution topography of the land surface and incorporates a green-wavelength laser operating at pulse rates of 2 to 10 kilohertz. Measurements from the laser-ranging device are coupled with data acquired from inertial navigation system (INS) attitude sensors and differentially corrected global positioning system (GPS) receivers to measure topography of the surface at accuracies of +/-15 centimeters. The nominal ATM platform is a Twin Otter or P-3 Orion aircraft, but the instrument may be deployed on a range of light aircraft. Elevation measurements were collected over the survey area using the ATM system, and the resulting data were then processed using the Airborne Lidar Processing System (ALPS), a custom-built processing system developed in a NASA-USGS collaboration. ALPS supports the exploration and processing of Lidar data in an interactive or batch mode. Modules for presurvey flight line definition, flight path plotting, Lidar raster and waveform investigation, and digital camera image playback have been developed. Processing algorithms have been developed to extract the range to the first and last significant return within each waveform. ALPS is routinely used to create

  1. Correlates of smoking quit attempts: Florida Tobacco Callback Survey, 2007

    Directory of Open Access Journals (Sweden)

    Dietz Noella

    2009-06-01

    Full Text Available Abstract Objective The public health burden of tobacco-associated diseases in the USA remains high, in part because many people's attempts to quit are unsuccessful. This study examined factors associated with having lifetime or recent attempts to quit smoking among current smokers, based on a telephone survey of Florida adults. Methods Data from the 2007 telephone-based Florida Behavioral Risk Factor Surveillance System (BRFSS and its follow-up survey, the Tobacco Callback Survey, were used to assess determinants of having ever attempted to quit smoking and attempted to quit smoking in the past 12 months. All analyses were conducted using SAS. Results Among 3,560 current smokers, 41.5% reported having tried to quit smoking in the past 12 months while 83.4% reported having ever tried to quit. Having a history of a tobacco-related medical condition was significantly associated with both recent (Adjusted Odds Ratio (AOR 1.41 [Confidence Interval 1.19–1.65] and lifetime quit attempts (AOR 1.43 [1.15–1.79]. Greater nicotine dependence and being advised by a physician to quit smoking were also positively associated with lifetime quit attempts. Receipt of healthcare provider advice to quit smoking in the past 12 months and a strong belief that quitting following a long history of regular smoking would not result in health benefits and belief that there are health benefits to quitting smoking were associated with lifetime quit attempts. Conclusion Targeted smoking cessation interventions are needed for smokers with selected medical conditions and with high nicotine dependence. The importance of physician advice in encouraging individuals to quit is further highlighted.

  2. Endosulfan in the atmosphere of South Florida: Transport to Everglades and Biscayne National Parks

    Science.gov (United States)

    Nutrient inputs from urban encroachment and agricultural activities have been implicated in contributing to the environmental health decline and loss of organism diversity of South Florida ecosystems. However, the frequent prophylactic application of pesticides is being examined as another possible...

  3. Coral Reef and Hardbottom from Unified Florida Reef Tract Map (NODC Accession 0123059)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is a subset of the Unified Map representing Coral reef and Hardbottom areas. Version 1.1 - December 2013. The Unified Florida Reef Tract Map (Unified...

  4. Broward County Florida Reef Track Thermographic Data Sept 2000 - Oct 2002, (NODC Accession 0000829)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Broward County Florida has responsibility for the resource management of coral reefs in marine waters adjacent to Broward County. The Department of Planning and...

  5. 77 FR 60749 - Endangered and Threatened Wildlife and Plants; Proposed Endangered Species Status for the Florida...

    Science.gov (United States)

    2012-10-04

    ... on reproduction and demography is sparse. The Florida bonneted bat has low fecundity; litter size is... ``found under rocks when bull-dozing ground'' (Timm and Genoways 2004, p. 860). A colony was found in...

  6. 2008 Northwest Florida Water Management District (NWFWMD) Lidar: Eglin Air Force Base, Walton County, FL

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — In the summer of 2008, the Northwest Florida Water Management District collected lidar data over a portion of Walton County, FL (Eglin Air force Base) to support...

  7. Coral and artificial reef shape files, Broward County, Florida, (NODC Accession 0000244)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Coral reef and artificial reef location shape files and accompanying table files for reefs located off shore of Broward County, Florida. Accompanying "attribute"...

  8. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Florida Panhandle: HABITATS (Habitat Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for rare plants for the Florida Panhandle. Vector polygons in this data set represent rare plant...

  9. Benthic Habitat Mapping - Indian River Lagoon, Florida Submerged Aquatic Vegetation (SAV) Data 1996 Biotic

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The NOAA Office for Coastal Management's Coastal Change Analysis Program, in cooperation with the St. Johns River and South Florida Water Management Districts, used...

  10. Calibrated EAARL-B Submerged Topography--Fort Lauderdale, Florida, 2014 (GEOID12A)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Binary point-cloud data of a portion of the submerged environs of Fort Lauderdale, Florida, were produced from remotely sensed, geographically referenced elevation...

  11. EAARL Coastal Topography--Western Florida, Post-Hurricane Charley, 2004: Seamless (Bare Earth and Submerged)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A seamless (bare-earth and submerged) elevation map (also known as a Digital Elevation Model, or DEM) of a portion of western Florida, post-Hurricane Charley, was...

  12. EAARL Coastal Topography--Western Florida, Post-Hurricane Charley, 2004: First Surface

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A first-surface elevation map (also known as a Digital Elevation Model, or DEM) of a portion of western Florida, post-Hurricane Charley, was produced from remotely...

  13. Bathymetry Mapping of the West Florida Shelf (Northern Region), Gulf of Mexico (NODC Accession 0001410)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XYZ ASCII format data generated from the 2001 multibeam sonar survey of the West Florida Shelf, Gulf of Mexico. The data include high-resolution bathymetry and...

  14. EAARL Coastal Topography--Eastern Florida, Post-Hurricane Jeanne, 2004: Bare Earth

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A digital elevation model (DEM) of a portion of the eastern Florida coastline, post-Hurricane Jeanne (September 2004 hurricane), was produced from remotely sensed,...

  15. EAARL Coastal Topography--Eastern Florida, Post-Hurricane Jeanne, 2004: First Surface

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A digital elevation model (DEM) of a portion of the eastern Florida coastline, post-Hurricane Jeanne (September 2004 hurricane), was produced from remotely sensed,...

  16. Florida Reef Fish Visual Census 1994 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  17. Florida Reef Fish Visual Census 1997 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  18. Florida Reef Fish Visual Census 1988 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  19. Florida Reef Fish Visual Census 1991 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  20. Florida Reef Fish Visual Census 1998 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  1. Florida Reef Fish Visual Census 1979 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  2. Florida Reef Fish Visual Census 1993 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  3. Florida Reef Fish Visual Census 1981 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  4. Florida Reef Fish Visual Census 1986 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  5. Florida Reef Fish Visual Census 1992 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  6. Florida Reef Fish Visual Census 1982 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  7. Florida Reef Fish Visual Census 1995 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  8. Florida Reef Fish Visual Census 1985 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  9. Florida Reef Fish Visual Census 1990 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  10. Florida Reef Fish Visual Census 1980 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  11. Florida Reef Fish Visual Census 1996 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  12. Florida Reef Fish Visual Census 1984 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  13. Florida Reef Fish Visual Census 1983 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  14. Florida Reef Fish Visual Census 1989 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  15. Florida Investigates 2 More Possible Cases of Zika Virus Local Transmission

    Science.gov (United States)

    ... html Florida Investigates 2 More Possible Cases of Zika Virus Local Transmission If all 4 are confirmed, they ... to see cases of local transmission of the Zika virus this summer in warm, humid southern states such ...

  16. Backscatter Mapping of the West Florida Shelf (Southern Region), Gulf of Mexico (NODC Accession 0001410)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XYZ ASCII format data generated from the 2001 multibeam sonar survey of the West Florida Shelf, Gulf of Mexico. The data include high-resolution bathymetry and...

  17. [Florida Panther National Wildlife Refuge: Comprehensive Conservation Plan]: Finding of No Significant Impact

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This is the FONSI that accompanies the Comprehensive Conservation Plan for Florida Panther NWR. This document provides a comprehensive review and analysis of the...

  18. 2004 Southwest Florida Water Management District (SWFWMD) Lidar Project: Pasco County (Classified)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Earthdata International was contracted to provide mapping services in Pasco County, Florida. Conventional aerial photography along with LIDAR observations were made....

  19. 76 FR 36150 - Notice of Inventory Completion: Florida Department of State/Division of Historical Resources...

    Science.gov (United States)

    2011-06-21

    ... assistance from the C.A. Pound Human Identification Laboratory and Dr. John Krigbaum, University of Florida... with the Archaic and Glades cultures of the area. The human remains are believed to be from the...

  20. 2006 Southwest Florida Water Management District (SWFWMD) Lidar: Upper Myakka District

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — EarthData International collected ALS-50-derived LiDAR over Upper Myakka Florida with a one-meter post spacing. The period of collection was between 3 October and...

  1. Hurricane Jeanne Aerial Photography: High-Resolution Imagery of the Atlantic Coast of Florida After Landfall

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The imagery posted on this site is of the Atlantic coast of Florida after Hurricane Jeanne made landfall. The regions photographed range along a 100-mile stretch...

  2. [Florida Panther National Wildlife Refuge: Comprehensive Conservation Plan]: Environmental Action Statement

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document states that the proposed action for the Florida Panther NWR is found not to have significant environmental effects as determined by the environmental...

  3. Hurricane Dennis Aerial Photography: Draft Image Mosaics of the Florida Panhandle and Surrounding Regions After Landfall

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The imagery posted on this site is of the Florida panhandle and surrounding regions after Hurricane Dennis made landfall. The regions photographed range from...

  4. Uncalibrated EAARL-B Submerged Topography--Fort Lauderdale, Florida, 2014 (GEOID12A)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Binary point-cloud data of a portion of the submerged environs of Fort Lauderdale, Florida, were produced from remotely sensed, geographically referenced elevation...

  5. Uncalibrated EAARL-B Submerged Topography--Fort Lauderdale, Florida, 2014 (WGS84)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Binary point-cloud data of a portion of the submerged environs of Fort Lauderdale, Florida, were produced from remotely sensed, geographically referenced elevation...

  6. EAARL-B Submerged Topography—Crocker Reef, Florida, 2014

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — A submerged topography digital elevation model (DEM) mosaic for a portion of the submerged environs of Crocker Reef, Florida, was produced from remotely sensed,...

  7. 2007 Florida Division of Emergency Management (FDEM) Lidar Project: Bay County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a topographic survey conducted for a coalition of GIS practitioners, including the Florida Division of...

  8. 2007 Florida Division of Emergency Management (FDEM) Lidar Project: Eastern Indian River County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a topographic survey conducted for a coalition of GIS practitioners, including the Florida Division of...

  9. Florida State Briefing Book for low-level radioactive-waste management

    Energy Technology Data Exchange (ETDEWEB)

    None

    1981-06-01

    The Florida State Briefing Book is one of a series of state briefing books on low-level radioactive waste management practices. It has been prepared to assist state and federal agency officials in planning for safe low-level radioactive waste disposal. The report contains a profile of low-level radioactive waste generators in Florida. The profile is the result of a survey of NRC licensees in Florida. The briefing book also contains a comprehensive assessment of low-level radioactive waste management issues and concerns as defined by all major interested parties including industry, government, the media, and interest groups. The assessment was developed through personal communications with representatives of interested parties, and through a review of media sources. Lastly, the briefing book provides demographic and socioeconomic data and a discussion of relevant government agencies and activities, all of which may impact waste management practices in Florida.

  10. 2005 Southwest Florida Water Management District (SWFWMD) LiDAR: Marion County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Digital terrain models (DTM) provide a 3-dimensional representation of the earth's surface. The DTM for the Southwest Florida Water Management District project was...

  11. Calibrated EAARL-B Submerged Topography--Fort Lauderdale, Florida, 2014 (WGS84)

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — Binary point-cloud data of a portion of the submerged environs of Fort Lauderdale, Florida, were produced from remotely sensed, geographically referenced elevation...

  12. Economic Development Through Biomass Systems Integration in Central Florida: Final Report; May 5, 1995

    Energy Technology Data Exchange (ETDEWEB)

    Stricker, J. A.; Smith, W. H.

    2004-07-01

    Reclaimed phosphate mined land in central Florida has been identified as an area with potential for growing biomass crops. Approximately 73,000 acres of land could be available for production. Additional research is needed to define the possibilities.

  13. Backscatter Mapping of the West Florida Shelf (Northern Region), Gulf of Mexico (NODC Accession 0001410)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XYZ ASCII format data generated from the 2001 multibeam sonar survey of the West Florida Shelf, Gulf of Mexico. The data include high-resolution bathymetry and...

  14. [Florida Panther National Wildlife Refuge: Comprehensive Conservation Plan]: Intra-Service Section 7 Consultation

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This is the Section 7 evaluation that accompanies the Comprehensive Conservation Plan for Florida Panther NWR. This document provides an analysis of how the...

  15. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: FISH (Fish Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine and estuarine fish species in South Florida. Vector polygons in this data set represent fish...

  16. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: INVERT (Invertebrate Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for marine and estuarine invertebrate species in South Florida. Vector polygons in this data set represent...

  17. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: REPTILES (Reptile Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for sea turtles, crocodiles, mangrove terrapins, and other rare species in [for] South Florida. Vector...

  18. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: ESIL (ESI Shoreline Types - Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The ESIL data set contains vector lines representing the shoreline and coastal habitats of South Florida classified according to the Environmental Sensitivity Index...

  19. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: ESIP (ESI Shoreline Types - Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The ESIP data set contains vector polygons representing the shoreline and coastal habitats of South Florida classified according to the Environmental Sensitivity...

  20. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: HABITATS (Habitat Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for threatened/endangered/rare terrestrial plants and communities in [for] South Florida. The terrestrial...

  1. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: South Florida: BENTHIC (Benthic Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains benthic habitats, including coral reef and hardbottom, seagrass, algae, and others in [for] South Florida. Vector polygons in the data set...

  2. 2007 Florida Division of Emergency Management (FDEM) Lidar Project: Nassau County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This report pertains to a Specific Purpose LiDAR Survey of Nassau County, Florida. The LiDAR aerial acquisition was conducted by Terrapoint USA between August 18,...

  3. Persistent Organochlorine Pesticides and their Metabolites in Alligator Livers from Lakes Apopka and Woodruff, Florida, USA

    Science.gov (United States)

    Reproductive disorders in American alligators (Alligator mississippiensis) inhabiting Lake Apopka, Florida, have been observed for several years. Such disorders are hypothesized to be caused by endocrine disrupting contaminants occurring in the Lake due to pesticide spills and ...

  4. 76 FR 29786 - Environmental Impact Statement for the Big Cypress National Preserve Addition, Florida

    Science.gov (United States)

    2011-05-23

    ... Big Cypress National Preserve Addition, Florida. On February 4, 2011, the Regional Director, Southeast... National Park Service Environmental Impact Statement for the Big Cypress National Preserve Addition... Management Plan/Environmental Impact Statement for the Big Cypress National Preserve Addition....

  5. 2007 Florida Division of Emergency Management (FDEM) Lidar Project: Taylor County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This Light Detection and Ranging (LiDAR) LAS dataset is a topographic survey conducted for a coalition of GIS practitioners, including the Florida Division of...

  6. Octocoral Density for the Florida Keys National Marine Sanctuary from 1999-2009. (NODC Accession 0123059)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — Using data collected from 1999 to 2009, the dataset includes species density of benthic octocorals collected from multiple habitat types across the south Florida...

  7. Multibeam Mapping of the West Florida Shelf, Gulf of Mexico, Twin Ridges Area

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — XYZ ASCII format high-resolution bathymetry data generated from the 2002 multibeam sonar survey of the West Florida Shelf, Gulf of Mexico.

  8. Florida Reef Fish Visual Census 1987 Species Site Matrix (NODC Accession 0001394)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is an ArcView shapefile set that contains locations of sampled coral reef fish species at the National Marine Sanctuary along the Florida Keys. The...

  9. Scleractinian Density for Florida Keys national Marine Sanctuary from 1999-2012 (NODC Accession 0123059)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset contains records of scleractinian colony density, within the Florida Keys National Marine Sanctuary, compiled from multiple sources. These are: SCREAM,...

  10. 2008 Florida Division of Emergency Management (FDEM) Lidar Project: Pasco County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is one component of a digital terrain model (DTM) for the Florida Division of Emergency Management's (FDEM) Project Management and Technical Services...

  11. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Florida Panhandle: ESIL (ESI Shoreline Types - Lines)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The ESIL data set contains vector lines representing the shoreline and coastal habitats of the Florida Panhandle, classified according to the Environmental...

  12. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Florida Panhandle: REPTPT (Reptile Points)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set contains sensitive biological resource data for threatened and endangered reptiles/amphibians for the Florida Panhandle. Vector points in this data set...

  13. Sensitivity of Coastal Environments and Wildlife to Spilled Oil: Florida Panhandle: ESIP (ESI Shoreline Types - Polygons)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The ESIP data set contains vector polygons representing the shoreline and coastal habitats of the Florida Panhandle, classified according to the Environmental...

  14. 77 FR 31240 - Approval and Promulgation of Air Quality Implementation Plans; State of Florida; Regional Haze...

    Science.gov (United States)

    2012-05-25

    ..., wilderness areas, and international parks meeting certain size criteria) in the western United States is 100...: Alabama, Florida, Georgia, Kentucky, Mississippi, North Carolina, South Carolina, Tennessee, Virginia... Environments (IMPROVE), Colorado State University, Cooperative Institute for Research in the Atmosphere,...

  15. 2006 Florida LiDAR: Escambia, Santa Rosa, and Walton Counties

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — ESCAMBIA: The Light Detection and Ranging (LiDAR) LAS dataset is a survey of select areas within Escambia County, Florida. These data were produced for Dewberry and...

  16. Quarterly report on the Florida Island Refuges for quarter ending September 30, 1940

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report for Florida Islands National Wildife Refuges outlines Refuge accomplishments from July through September of 1940. The report begins by...

  17. 2007 Southwest Florida Water Management District (SWFWMD) LiDAR: Hernando County

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This dataset is one component of a digital terrain model (DTM) for the Southwest Florida Water Management Districts FY2006 Digital Orthophoto (B089) and LiDAR...

  18. Quarterly report on the Florida Island Refuges for quarter ending March 31, 1940

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This narrative report for Florida Islands National Wildife Refuges outlines Refuge accomplishments from January through March of 1940. The report begins by...

  19. 2005 St. Johns River Water Management District (SJRWMD) Lidar: Western Seminole County, Florida

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set consists of a bare earth data set of 498 files covering a geographic area of 175 square miles in western Seminole County, Florida and includes small...

  20. 2005 Southwest Florida Water Management District (SWFWMD) LiDAR: Weeki Wachee

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — This data set is one component of a digital terrain model (DTM) for Weeki Wachee, Hernando County, Florida encompassing approximately 13.5 square miles. This dataset...