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Sample records for amboinicus lour spreng

  1. Aktivitas Antioksidan Ekstrak Etanol Daun Bangun-bangun (Plectranthus amboinicus (Lour.) Spreng.)

    OpenAIRE

    Sartika, Dewi

    2015-01-01

    Plectranthus amboinicus (Lour.) Spreng. contain potential antioxidants such as flavonoids and polyphenols. The recent studies said that Plectranthus amboinicus (Lour.) Spreng. has anti-inflammatory effect, hepatoprotective, radioprotektive and protect chromosome damage. these effects caused by antioxidant properties that can prevent free radical damage to the body cells. Antioxidant activity of ethanol extracts of Plectranthus amboinicus (Lour.) Spreng. The antioxidant activity of ethanol...

  2. Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance

    OpenAIRE

    Greetha Arumugam; Mallappa Kumara Swamy; Uma Rani Sinniah

    2016-01-01

    Plectranthus amboinicus (Lour.) Spreng. is a perennial herb belonging to the family Lamiaceae which occurs naturally throughout the tropics and warm regions of Africa, Asia and Australia. This herb has therapeutic and nutritional properties attributed to its natural phytochemical compounds which are highly valued in the pharmaceutical industry. Besides, it has horticultural properties due to its aromatic nature and essential oil producing capability. It is widely used in folk medicine to trea...

  3. Pharmacognostical studies on the leaves of Plectranthus amboinicus (Lour Spreng

    Directory of Open Access Journals (Sweden)

    Kaliappan Nirmala

    2008-01-01

    Full Text Available Plectranthus amboinicus (Lour Spreng belonging to the family Lamiaceae commonly known as Karpuravalli, Omavalli in Tamil, Patta ajavayin, Patharcur in Hindi, Country borage in English is a large succulent aromatic perennial herb. It is highly aromatic pubescent herb with distinctive smelling leaves. The plant is distributed throughout in India, cultivated in the gardens. The leaves of this plant have been used in malarial fever, hepatopathy, renal and vesical calculi, cough, chronic asthma, hiccough, bronchitis, anthelmintic, colic and convulsions. This paper deals with the micro morphological studies carried out on the leaves of Plectranthus amboinicus one of the WHO accepted parameter for identification of medicinal plants. For the better understanding of structural details Scanning Electron Microscopy (SEM also employed.

  4. Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance.

    Science.gov (United States)

    Arumugam, Greetha; Swamy, Mallappa Kumara; Sinniah, Uma Rani

    2016-01-01

    Plectranthus amboinicus (Lour.) Spreng. is a perennial herb belonging to the family Lamiaceae which occurs naturally throughout the tropics and warm regions of Africa, Asia and Australia. This herb has therapeutic and nutritional properties attributed to its natural phytochemical compounds which are highly valued in the pharmaceutical industry. Besides, it has horticultural properties due to its aromatic nature and essential oil producing capability. It is widely used in folk medicine to treat conditions like cold, asthma, constipation, headache, cough, fever and skin diseases. The leaves of the plant are often eaten raw or used as flavoring agents, or incorporated as ingredients in the preparation of traditional food. The literature survey revealed the occurrence 76 volatiles and 30 non-volatile compounds belonging to different classes of phytochemicals such as monoterpenoids, diterpenoids, triterpenoids, sesquiterpenoids, phenolics, flavonoids, esters, alcohols and aldehydes. Studies have cited numerous pharmacological properties including antimicrobial, antiinflammatory, antitumor, wound healing, anti-epileptic, larvicidal, antioxidant and analgesic activities. Also, it has been found to be effective against respiratory, cardiovascular, oral, skin, digestive and urinary diseases. Yet, scientific validation of many other traditional uses would be appreciated, mainly to discover and authenticate novel bioactive compounds from this herb. This review article provides comprehensive information on the botany, phytochemistry, pharmacology and nutritional importance of P. amboinicus essential oil and its various solvent extracts. This article allows researchers to further explore the further potential of this multi-utility herb for various biomedical applications. PMID:27043511

  5. Plectranthus amboinicus (Lour. Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance

    Directory of Open Access Journals (Sweden)

    Greetha Arumugam

    2016-03-01

    Full Text Available Plectranthus amboinicus (Lour. Spreng. is a perennial herb belonging to the family Lamiaceae which occurs naturally throughout the tropics and warm regions of Africa, Asia and Australia. This herb has therapeutic and nutritional properties attributed to its natural phytochemical compounds which are highly valued in the pharmaceutical industry. Besides, it has horticultural properties due to its aromatic nature and essential oil producing capability. It is widely used in folk medicine to treat conditions like cold, asthma, constipation, headache, cough, fever and skin diseases. The leaves of the plant are often eaten raw or used as flavoring agents, or incorporated as ingredients in the preparation of traditional food. The literature survey revealed the occurrence 76 volatiles and 30 non-volatile compounds belonging to different classes of phytochemicals such as monoterpenoids, diterpenoids, triterpenoids, sesquiterpenoids, phenolics, flavonoids, esters, alcohols and aldehydes. Studies have cited numerous pharmacological properties including antimicrobial, antiinflammatory, antitumor, wound healing, anti-epileptic, larvicidal, antioxidant and analgesic activities. Also, it has been found to be effective against respiratory, cardiovascular, oral, skin, digestive and urinary diseases. Yet, scientific validation of many other traditional uses would be appreciated, mainly to discover and authenticate novel bioactive compounds from this herb. This review article provides comprehensive information on the botany, phytochemistry, pharmacology and nutritional importance of P. amboinicus essential oil and its various solvent extracts. This article allows researchers to further explore the further potential of this multi-utility herb for various biomedical applications.

  6. In-vitro anti- inflammatory activity of aqueous extract of leaves of Plectranthus amboinicus (Lour.) Spreng

    OpenAIRE

    Ravikumar, V.R.; Dhanamani, M.; Sudhamani, T.

    2009-01-01

    Aqueous extract of leaves of Plectranthus amboinicus (lour.) Spreng, which is traditionally used in the treatment of cough and cold was screened for its anti- inflammatory activity by HRBC membrane stabilisation model. Aqueous extract (500 mcg/ml) showed significant anti-inflammatory activity as compared to that of hydrocortisone sodium.

  7. In-vitro anti- inflammatory activity of aqueous extract of leaves of Plectranthus amboinicus (Lour.) Spreng.

    Science.gov (United States)

    Ravikumar, V R; Dhanamani, M; Sudhamani, T

    2009-04-01

    Aqueous extract of leaves of Plectranthus amboinicus (lour.) Spreng, which is traditionally used in the treatment of cough and cold was screened for its anti- inflammatory activity by HRBC membrane stabilisation model. Aqueous extract (500 mcg/ml) showed significant anti-inflammatory activity as compared to that of hydrocortisone sodium. PMID:22557324

  8. Constituintes voláteis de Mentha pulegium L. e Plectranthus amboinicus (Lour.) Spreng Volatile constituents of Mentha pulegium L. and Plectranthus amboinicus (Lour.) Spreng

    OpenAIRE

    R.A. Oliveira; I.C.G Sá; Duarte, L P; F.F Oliveira

    2011-01-01

    Nesse trabalho foi investigado a composição química de óleos essenciais de duas plantas medicinais cultivadas em Ilhéus, Brasil, Plectranthus amboinicus (Lour.) Spreng. (hortelã-grosso) e Mentha pulegium L. (poejo). Os óleos essenciais foram extraídos das folhas frescas por hidrodestilação e foram analisados por CG/FID e CG-EM, na primavera e no inverno. P. amboinicus forneceu, tanto na primavera como no inverno, 0,10% de óleo sendo timol o componente majoritário. M. pulegium forneceu na prim...

  9. Pharmacognostical studies on the leaves of Plectranthus amboinicus (Lour) Spreng

    OpenAIRE

    Kaliappan Nirmala; Viswanathan Periyanayagam

    2008-01-01

    Plectranthus amboinicus (Lour) Spreng belonging to the family Lamiaceae commonly known as Karpuravalli, Omavalli in Tamil, Patta ajavayin, Patharcur in Hindi, Country borage in English is a large succulent aromatic perennial herb. It is highly aromatic pubescent herb with distinctive smelling leaves. The plant is distributed throughout in India, cultivated in the gardens. The leaves of this plant have been used in malarial fever, hepatopathy, renal and vesical calculi, cough, chronic asthma, ...

  10. Antibacterial effects of Plectranthus amboinicus (Lour.) Spreng (Lamiaceae) in Methicillin resistant staphylococcus aureus (MRSA)

    OpenAIRE

    Gurgel, Ana P.A.D.; Silva, Jackeline G. da; Grangeiro, Ana R.S.; Xavier, Haroudo Satiro; Oliveira, Rinalda Araújo Guerra de; Maria S.V. Pereira; Ivone A. Souza

    2009-01-01

    The present study describes some in vitro experiments with hydroalcoholic extract of leaves from Plectranthus amboinicus (Lour.) Spreng (Lamiaceae) in several Methicillin resistant Staphylococcus aureus (MRSA) strains in the attempt to determine whether the popular use corroborates with pharmacological properties. The antimicrobial activity was determined by the agar diffusion assay method. The evaluation of the minimum inhibitory concentration was determined using the agar dilution method...

  11. Isolasi Senyawa Flavonoida Dari Daun Tumbuhan Bangun-Bangun(Plectranthus Amboinicus (Lour.) Spreng.)

    OpenAIRE

    Naiborhu, Putri N. E.

    2015-01-01

    Isolation of flavonoid compounds from leaves of bangun-bangun (P.amboinicus (Lour.) Spreng.) has been done with maceration by methanol solvent. The concentrated extract then dissolved with aethyl acetate until the solution clear. The concentrated extract of aethyl acetate then dissolved with methanol and partition extracted with N-hexane. The concentrated extract of methanol then acided by HCl 6% while heated. The filtrate continue partition extracted with chloroform to get the flavonoid comp...

  12. Diuretic activity of leaves of Plectranthus amboinicus (Lour) Spreng in male albino rats

    OpenAIRE

    Roshan Patel; Mahobia, Naveen K.; Ravindra Gendle; Basant Kaushik; Sudarshan K Singh

    2010-01-01

    The shade-dried powder of leaves of Plectranthus amboinicus (Lour) Spreng was subjected to successive extraction using the various solvents (petroleum ether, chloroform, ethanol and water) in increasing order of polarity. The preliminary phytochemical analyses were carried out for all the extracts. The analyses of the leaves revealed the presence of alkaloids, carbohydrates, glycosides, proteins, amino acids, flavonoids, quinine, tannins, phenolic compounds and terpenoids. Since the phytocons...

  13. Induction of Apoptosis in Human Colon Cancer Cells by Methanol Fraction of Leaves of Plectranthus amboinicus (Lour Spreng

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    Preeja G Pillai

    2013-08-01

    Full Text Available To evaluate the cytotoxicity and apoptosis inducing activities of the methanol extract from leaves of Plectranthus amboinicus (Lour Spreng in an attempt to determine whether the medicinal uses are supported by pharmacological effects. Cytotoxicity was determined by sulforhodamine B assay method. Cytotoxicity and apoptosis inducing effect were evaluated in- vitro using human colon cancer cell line, COLO 205. There was statistically significant cell growth inhibition at the doses of 10, 20, 40 and 80 µg/ml methanol extract of Plectranthus amboinicus (Lour Spreng. Similarly, Plectranthus amboinicus at the doses of 50 and 100 µg/ml induced apoptosis in COLO 205 cells. The results suggest that the methanol extract of Plectranthus amboinicus (Lour Spreng possesses anti- proliferative and apoptosis inducing activities, supporting the folk use of this medicinal species.

  14. Constituintes voláteis de Mentha pulegium L. e Plectranthus amboinicus (Lour. Spreng Volatile constituents of Mentha pulegium L. and Plectranthus amboinicus (Lour. Spreng

    Directory of Open Access Journals (Sweden)

    R.A Oliveira

    2011-01-01

    Full Text Available Nesse trabalho foi investigado a composição química de óleos essenciais de duas plantas medicinais cultivadas em Ilhéus, Brasil, Plectranthus amboinicus (Lour. Spreng. (hortelã-grosso e Mentha pulegium L. (poejo. Os óleos essenciais foram extraídos das folhas frescas por hidrodestilação e foram analisados por CG/FID e CG-EM, na primavera e no inverno. P. amboinicus forneceu, tanto na primavera como no inverno, 0,10% de óleo sendo timol o componente majoritário. M. pulegium forneceu na primavera 0,20% de óleo e no inverno 0,09%. Os componentes majoritários dos óleos foram pulegona e trans-cariofileno; borneol, mentol e piperitona foram identificados em menores quantidades. No inverno foram observados maiores quantidades de mentol e isomentol. Acetatos de neoisomentila, de mentila e de isometila foram observados somente no inverno. Esse trabalho contribui para o conhecimento das espécies vegetais cultivadas na região do sul da BahiaThis work investigated the chemical composition of the essential oils of two medicinal plants grown in Ilhéus Municipality, Bahia State, Brazil: Plectranthus amboinicus (Lour. Spreng (Mexican mint and Mentha pulegium L. (pennyroyal. The essential oils were extracted from fresh leaves by hydrodistillation and analyzed by GC-FID and GC-MS, in the spring and in the winter. In both seasons, P. amboinicus yielded 0.10% oil and had thymol as major component. M. pulegium yielded 0.20% and 0.09% oil in the spring and winter, respectively. The major components were pulegone and trans-caryophyllene, whereas borneol, menthol and piperitone were identified at small quantities. Higher menthol and isomenthol levels were detected in the winter. In addition, neoisomenthyl, menthyl and isomenthyl acetates were only observed in the winter. This work contributes to the knowledge of plant species grown in southern Bahia

  15. Analgesic and Antiinflammatory Activities of the Aqueous Extract from Plectranthus amboinicus (Lour.) Spreng. Both In Vitro and In Vivo

    OpenAIRE

    Yung-Jia Chiu; Tai-Hung Huang; Chuan-Sung Chiu; Tsung-Chun Lu; Ya-Wen Chen; Wen-Huang Peng; Chiu-Yuan Chen

    2012-01-01

    Plectranthus amboinicus (Lour.) Spreng. is a native Labiatae plant of Taiwan. The plants are commonly used in Chinese folk medicine for the treatment of cough, fever, sore throats, mumps, and mosquito bite. The aim of this study was to investigate the analgesic and antiinflammatory properties of the aqueous extract from Plec...

  16. Efek Antiproliferatif Ekstrak N-Heksan Daun Bangun-Bangun (Plectranthus amboinicus, (Lour.) Spreng.) Terhadap Sel MCF7

    OpenAIRE

    Hasibuan, Poppy Anjelisa Z.; Rosidah; Ilyas, Syafruddin; Nasution, M.Pandapotan

    2016-01-01

    Mutasi onkogen dapat memicu terjadinya suatu mekanisme untuk membatasi perluasan atau perbanyakan sel, dengan penekanan proliferasi. Pada kondisi kanker, terjadi gangguan keseimbangan antara apoptosis dan proliferasi sehingga besar kemungkinan akan menyebabkan progresi tumor. Daun Bangun-Bangun (Plectranthus amboinicus, (Lour.) Spreng.) mengandung triterpenoid yang berkhasiat antikanker. Penelitian ini bertujuan untuk menguji kinetika proliferasi sel kanker payudara MCF7 akibat pe...

  17. Diuretic activity of leaves of Plectranthus amboinicus (Lour Spreng in male albino rats

    Directory of Open Access Journals (Sweden)

    Roshan Patel

    2010-01-01

    Full Text Available The shade-dried powder of leaves of Plectranthus amboinicus (Lour Spreng was subjected to successive extraction using the various solvents (petroleum ether, chloroform, ethanol and water in increasing order of polarity. The preliminary phytochemical analyses were carried out for all the extracts. The analyses of the leaves revealed the presence of alkaloids, carbohydrates, glycosides, proteins, amino acids, flavonoids, quinine, tannins, phenolic compounds and terpenoids. Since the phytoconstituents present in the ethanolic and aqueous extracts were similar, both the extracts were selected for further study. The diuretic properties of ethanolic and aqueous extracts were evaluated by determination of urine volume and electrolyte concentration in male albino rats. Furosemide (10 mg/kg was used as standard while normal saline (0.9% was used as control. Both ethanolic and aqueous extracts (500 mg/kg have shown significant increase in the volume of urine and urinary concentration of Na, K and Cl ions. Thus, from the is study it may be concluded that the leaves of P. amboinicus (Lour Spreng possess diuretic activities.

  18. Diuretic activity of leaves of Plectranthus amboinicus (Lour) Spreng in male albino rats.

    Science.gov (United States)

    Patel, Roshan; Mahobia, Naveen K; Gendle, Ravindra; Kaushik, Basant; Singh, Sudarshan K

    2010-03-01

    The shade-dried powder of leaves of Plectranthus amboinicus (Lour) Spreng was subjected to successive extraction using the various solvents (petroleum ether, chloroform, ethanol and water) in increasing order of polarity. The preliminary phytochemical analyses were carried out for all the extracts. The analyses of the leaves revealed the presence of alkaloids, carbohydrates, glycosides, proteins, amino acids, flavonoids, quinine, tannins, phenolic compounds and terpenoids. Since the phytoconstituents present in the ethanolic and aqueous extracts were similar, both the extracts were selected for further study. The diuretic properties of ethanolic and aqueous extracts were evaluated by determination of urine volume and electrolyte concentration in male albino rats. Furosemide (10 mg/kg) was used as standard while normal saline (0.9%) was used as control. Both ethanolic and aqueous extracts (500 mg/kg) have shown significant increase in the volume of urine and urinary concentration of Na, K and Cl ions. Thus, from the is study it may be concluded that the leaves of P. amboinicus (Lour) Spreng possess diuretic activities. PMID:21808546

  19. Variability in growth, nutrition and phytochemical constituents of Plectranthus amboinicus (Lour) Spreng. as influenced by indigenous arbuscular mycorrhizal fungi

    OpenAIRE

    Sevanan Rajeshkumar

    2008-01-01

    A study was conducted under greenhouse nursery condition on the efficacy of seven indigenous arbuscular mycorrhizal (AM) fungi in the improvement of growth, biomass, nutrition and phytochemical constituents, namely total phenols, ortho dihydroxy phenols, flavonoids, alkaloids, tannins and saponins, in the roots and leaves of Plectranthus amboinicus (Lour) Spreng. Seedlings were raised in polythene bags containing soil inoculated with isolates of seven different indigenous AM fungi, viz. Acaul...

  20. Induction of Apoptosis in Human Breast Cancer (MCF7) Cells by n-Hexane Extract of Plectranthus amboinicus (Lour.) Spreng.

    OpenAIRE

    Hasibuan, Poppy Anjelisa Z.

    2016-01-01

    The n-hexane extract of Plectranthus amboinicus, (Lour.) Spreng. reduced the proliferation of MCF7 cells. The present study was carried out to evaluate the effect of the extract on human breast cancer cells viability and apoptosis. To detect apoptotic cells, MCF7 cells were stained with etydium bromide-acrydine orange (double staining method). Quantitative detectin of apoptotic cells was performed by fluorescens microscope. The growth of MCF7 was inhibited by treatment with n-h...

  1. Induction of Apoptosis in Human Colon Cancer Cells by Methanol Fraction of Leaves of Plectranthus amboinicus (Lour) Spreng

    OpenAIRE

    Preeja G Pillai; Suresh, P; Gitanjali Mishra

    2013-01-01

    To evaluate the cytotoxicity and apoptosis inducing activities of the methanol extract from leaves of Plectranthus amboinicus (Lour) Spreng in an attempt to determine whether the medicinal uses are supported by pharmacological effects. Cytotoxicity was determined by sulforhodamine B assay method. Cytotoxicity and apoptosis inducing effect were evaluated in- vitro using human colon cancer cell line, COLO 205. There was statistically significant cell growth inhibition at the doses of 10, 20, 40...

  2. Combination Effect of Ethylacetate Extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin Againts HeLa Cell Lines

    OpenAIRE

    Hasibuan, Poppy Anjelisa Z.; Rosidah

    2016-01-01

    The aims of the study were to investigate the growth-inhibiting mediating effect of Plectranthus amboinicus (Lour.) Spreng. ethylacetate extract (PAE) in combination therapy with doxorubicin againts HeLa cell lines, to analyzed the apoptotic induction and expression of cyclin D1, Bcl2 and COX-2 (cyclooxigenase-2) proteins of HeLa cell lines after treatment of PAE. The cytotoxicity effects were determined by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromi...

  3. Efficacy of Plectranthus amboinicus (Lour.) Spreng in a Murine Model of Methicillin-Resistant Staphylococcus aureus Skin Abscesses

    OpenAIRE

    Francisco Fábio Martins de Oliveira; Alba Fabiola Torres; Thially Braga Gonçalves; Gilvandete Maria Pinheiro Santiago; Cibele Barreto Mano de Carvalho; Milena Braga Aguiar; Lilia Maria Carneiro Camara; Silvia Helena Rabenhorst; Alice Maria Costa Martins; José Telmo Valença Junior; Aparecida Tiemi Nagao-Dias

    2013-01-01

    The present work aimed to evaluate the effectiveness of Plectranthus amboinicus (Lour.) Spreng against MRSA clinical isolates. The in vitro antimicrobial activity of the hydroalcoholic extract (HE), the ethyl acetate (EA) fraction and its subfractions were determined by broth microdilution and bioautography against MRSA clinical isolates. The microdilution checkerboard method was used to assess in vitro drug combination studies. To induce abscess formation, bacterial suspensions were added to...

  4. Analgesic and Antiinflammatory Activities of the Aqueous Extract from Plectranthus amboinicus (Lour. Spreng. Both In Vitro and In Vivo

    Directory of Open Access Journals (Sweden)

    Yung-Jia Chiu

    2012-01-01

    Full Text Available Plectranthus amboinicus (Lour. Spreng. is a native Labiatae plant of Taiwan. The plants are commonly used in Chinese folk medicine for the treatment of cough, fever, sore throats, mumps, and mosquito bite. The aim of this study was to investigate the analgesic and antiinflammatory properties of the aqueous extract from Plectranthus amboinicus (PA in vivo and in vitro. PA inhibited pain induced by acetic acid and formalin, and inflammation induced by carrageenan. The anti-inflammatory effect of PA was related to modulating antioxidant enzymes' activities in the liver and decreasing the Malondialdehyde (MDA level and the production of tumor necrosis factor alpha (TNF-α, and cyclooxygenase2 (COX-2 in edema-paw tissue in mice. In vitro studies show that PA inhibited the proinflammatory mediators in RAW 264.7 cells stimulated with lipopolysaccharide (LPS. PA blocked the degradation of IκB-α and nuclear translocation of NF-κB p65 subunit. Finally, the amount of carvacrol in the aqueous extract of PA was 1.88 mg/g extract. Our findings suggest that PA has analgesic and anti-inflammatory activities. These effects were mediated by inhibiting the proinflammatory mediators through blocking NF-κB activation. Meanwhile, the effects observed in this study provide evidence for folkloric uses of Plectranthus amboinicus (Lour. Spreng. in relieving pain and inflammation.

  5. Analgesic and Antiinflammatory Activities of the Aqueous Extract from Plectranthus amboinicus (Lour.) Spreng. Both In Vitro and In Vivo.

    Science.gov (United States)

    Chiu, Yung-Jia; Huang, Tai-Hung; Chiu, Chuan-Sung; Lu, Tsung-Chun; Chen, Ya-Wen; Peng, Wen-Huang; Chen, Chiu-Yuan

    2012-01-01

    Plectranthus amboinicus (Lour.) Spreng. is a native Labiatae plant of Taiwan. The plants are commonly used in Chinese folk medicine for the treatment of cough, fever, sore throats, mumps, and mosquito bite. The aim of this study was to investigate the analgesic and antiinflammatory properties of the aqueous extract from Plectranthus amboinicus (PA) in vivo and in vitro. PA inhibited pain induced by acetic acid and formalin, and inflammation induced by carrageenan. The anti-inflammatory effect of PA was related to modulating antioxidant enzymes' activities in the liver and decreasing the Malondialdehyde (MDA) level and the production of tumor necrosis factor alpha (TNF-α), and cyclooxygenase2 (COX-2) in edema-paw tissue in mice. In vitro studies show that PA inhibited the proinflammatory mediators in RAW 264.7 cells stimulated with lipopolysaccharide (LPS). PA blocked the degradation of IκB-α and nuclear translocation of NF-κB p65 subunit. Finally, the amount of carvacrol in the aqueous extract of PA was 1.88 mg/g extract. Our findings suggest that PA has analgesic and anti-inflammatory activities. These effects were mediated by inhibiting the proinflammatory mediators through blocking NF-κB activation. Meanwhile, the effects observed in this study provide evidence for folkloric uses of Plectranthus amboinicus (Lour.) Spreng. in relieving pain and inflammation. PMID:21915187

  6. Combination Effect Of N-Hexane Extract Of Plectranthus Amboinicus (LOUR.) Spreng. With Doxorubicin Againts HeLa Cell Lines

    OpenAIRE

    Rosidah; Hasibuan, Poppy Anjelisa Z.

    2016-01-01

    The study was aimed to investigate the growth inhibiting effect of Plectranthus amboinicus, (Lour.) Spreng n-hexane extract (PAN) in combination with doxorubicin againts HeLa cell Lines, to observe the apoptotic induction and immunocytochemistry of HeLa cell Lines on cyclin D1, Bcl-2, and Cox-2 after treatment of PAN. The percentage viability of the cell were carried out by using MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The effect of apoptosis...

  7. Cell Cycle Inhibition from Ethylacetate Extracts of Plectranthus amboinicus, (Lour.) Spreng.) Leaves on HeLa Cells Lines

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    Rosidah; Hasibuan, Poppy Anjelisa Z.; Satria, Denny

    2016-01-01

    Objective: To evaluate the effects of ethylacetate extract (EAE) of Plectranthus amboinicus (Lour.) Spreng.) leaves on cell cyle on HeLa cell lines. Methods: Analysis of cell cycle distribution was performed using flowcytometer and the data was analyzed using ModFit LT 3.0 program. Results: The EAE changes the accumulation of cell cycle phase from G0 -G1 phase (54.61%) to sub-G1 phase (69.73%). Conclusions: Based on the results, EAE is potential to be developed as co-chemoth...

  8. Analisa Komponen Kimia Minyak Atsiri dari Daun Bangun−Bangun (Plectranthus amboinicus (lour) spreng) Secara GC−MS

    OpenAIRE

    Lestari, Ade Surya Ayu

    2015-01-01

    Telah dilakukan penelitian tentang kandungan minyak atsiri yang terkandung dalam daun Bangun-bangun (Plectranthus amboinicus (Lour) Spreng), ini dilakukan dengan metode destilasi air dengan memakai alat Stahl. Adapun hasil yang diperoleh dengan alat GC-MS (Gas kromatografi-Spektrum massa) adalah Puncak dengan RT 12,724 menit merupakan senyawa dengan rumus molekul C15-H24 senyawa berupa Alpha-Gurjunene (1,33%), Puncak dengan RT 14,492 menit merupakan senyawa dengan rumus molekul C15¬H24 senyaw...

  9. Formulasi Sediaan Gel dari Ekstrak Etanol Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) dan Uji Aktivitasnya terhadap Beberapa Bakteri Penyebab Jerawat

    OpenAIRE

    Bancin, Juni Dariyatyi

    2014-01-01

    Plectranthy leaves (Plectranthus amboinicus (Lour.) Spreng) have activity as antibacterial of some microbes. Plectranthy leaves contain saponin, flavonoid and steroid, that potentially act as antibacterial. The purpose of this research to formulate and test gel of Plectranthy leaves ethanol extract as anti acne stability and test antibacterial cause acne (Propionibacterium acne and Staphylococcus epidermidis). The research included: identification, characterization and ph...

  10. Variability in growth, nutrition and phytochemical constituents of Plectranthus amboinicus (Lour Spreng. as influenced by indigenous arbuscular mycorrhizal fungi

    Directory of Open Access Journals (Sweden)

    Sevanan Rajeshkumar

    2008-07-01

    Full Text Available A study was conducted under greenhouse nursery condition on the efficacy of seven indigenous arbuscular mycorrhizal (AM fungi in the improvement of growth, biomass, nutrition and phytochemical constituents, namely total phenols, ortho dihydroxy phenols, flavonoids, alkaloids, tannins and saponins, in the roots and leaves of Plectranthus amboinicus (Lour Spreng. Seedlings were raised in polythene bags containing soil inoculated with isolates of seven different indigenous AM fungi, viz. Acaulospora bireticulata, A. scrobiculata, Gigaspora margarita, Glomus aggregatum, G. mosseae, G. geosporum, and Scutellospora heterogama. P. amboinicus seedlings raised in the presence of AM fungi generally showed an increase in plant growth, nutritional status and phytochemical constituents over those grown in the absence of AM fungi. The extent of growth, biomass, nutritional status and phytochemical constituents enhanced by AM fungi varied with the species of AM fungi inhabiting the roots and leaves of P. amboinicus seedlings. Considering the various plant growth parameters, nutritional status of the plant, total phenols, ortho dihydroxy phenols, alkaloids , flavonoids , tannins, and saponins in the roots and leaves, it was observed that Gigaspora margarita is the best AM symbiont for P. amboinicus used in this experiment.

  11. Pengaruh Ekstrak Etanol Daun Bangun-Bangun (Plectranthus Amboinicus, (Lour.) Spreng) Terhadap Penghambatan Degranulasi Mastosit Yang Tersensititasi Aktif Pada Mencit Jantan Secara In Vitro

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    Ronal

    2013-01-01

    Mastocyte is important source in immune response is responsible for the occurrence of allergic reaction. Allergic reaction are caused by the bond between the antigen and antibody Immunoglobulin E (IgE) which will cause the degranulation of mastocyte and basophil and thereby releasing chemical mediators such as histamine, prostaglandins, bradykinin, arachidonic acid. Bangun-bangun (Plectranthus amboinicus, (Lour.) Spreng is a plant and its leaf has an activity as antiallergy. The objective of ...

  12. Efek Ekstrak Etanol Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) Terhadap Kadar Nitro Oxide Pada Tikus Jantan yang Diinduksi Doksorubisin

    OpenAIRE

    Furaida

    2015-01-01

    Doxorubicin is one of anthracycline class used in cancer treatment, but its use is limited due to its cardiotoxicity that is caused by increased nitric oxide (NO) level. The leaves of Plecranthus amboinicus (Lour.) Spreng of the family of the family Lamiaceace has antioxidant property which can delay or inhibit oxidation so that prevent the cell damage caused by free radicals. This study aimed to determine the effect of ethanol extract of bangun-bangun leaves on blood nitric oxide level in do...

  13. Efficacy of Plectranthus amboinicus (Lour. Spreng in a Murine Model of Methicillin-Resistant Staphylococcus aureus Skin Abscesses

    Directory of Open Access Journals (Sweden)

    Francisco Fábio Martins de Oliveira

    2013-01-01

    Full Text Available The present work aimed to evaluate the effectiveness of Plectranthus amboinicus (Lour. Spreng against MRSA clinical isolates. The in vitro antimicrobial activity of the hydroalcoholic extract (HE, the ethyl acetate (EA fraction and its subfractions were determined by broth microdilution and bioautography against MRSA clinical isolates. The microdilution checkerboard method was used to assess in vitro drug combination studies. To induce abscess formation, bacterial suspensions were added to Citodex and inoculated subcutaneously into male Swiss mice. The treatment protocol consisted of 2 doses of HE, the EA fraction or vancomycin introduced intraperitoneally into mice 3 and 12 h after infection. The EA fraction and its subfractions presented the lowest minimal inhibitory concentrations (MIC, 0.25 to 0.5 mg/mL. The plant samples were bacteriostatic at 2x and 4x MIC and bactericidal at 100 mg/mL. The EA fraction presented synergism with vancomycin and an additive effect with ciprofloxacin. A significant reduction of abscess volume, bacterial cell counts in abscess slurries, and inflammatory scores was observed in the HE and EA fraction-treated groups. The samples were effective in treating the animals in a dose-dependent fashion. The present study proved the effectiveness of P. amboinicus fractions against MRSA using in vitro and in vivo assays.

  14. Efficacy of Plectranthus amboinicus (Lour.) Spreng in a Murine Model of Methicillin-Resistant Staphylococcus aureus Skin Abscesses.

    Science.gov (United States)

    de Oliveira, Francisco Fábio Martins; Torres, Alba Fabiola; Gonçalves, Thially Braga; Santiago, Gilvandete Maria Pinheiro; de Carvalho, Cibele Barreto Mano; Aguiar, Milena Braga; Camara, Lilia Maria Carneiro; Rabenhorst, Silvia Helena; Martins, Alice Maria Costa; Valença Junior, José Telmo; Nagao-Dias, Aparecida Tiemi

    2013-01-01

    The present work aimed to evaluate the effectiveness of Plectranthus amboinicus (Lour.) Spreng against MRSA clinical isolates. The in vitro antimicrobial activity of the hydroalcoholic extract (HE), the ethyl acetate (EA) fraction and its subfractions were determined by broth microdilution and bioautography against MRSA clinical isolates. The microdilution checkerboard method was used to assess in vitro drug combination studies. To induce abscess formation, bacterial suspensions were added to Citodex and inoculated subcutaneously into male Swiss mice. The treatment protocol consisted of 2 doses of HE, the EA fraction or vancomycin introduced intraperitoneally into mice 3 and 12 h after infection. The EA fraction and its subfractions presented the lowest minimal inhibitory concentrations (MIC, 0.25 to 0.5 mg/mL). The plant samples were bacteriostatic at 2x and 4x MIC and bactericidal at 100 mg/mL. The EA fraction presented synergism with vancomycin and an additive effect with ciprofloxacin. A significant reduction of abscess volume, bacterial cell counts in abscess slurries, and inflammatory scores was observed in the HE and EA fraction-treated groups. The samples were effective in treating the animals in a dose-dependent fashion. The present study proved the effectiveness of P. amboinicus fractions against MRSA using in vitro and in vivo assays. PMID:23533472

  15. Pengaruh Ekstrak Etanol Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) Terhadap Penghambatan Karsinogenesis Mammae Mencit Betina Yang Diinduksi Benzo(α)piren

    OpenAIRE

    Samosir, Arnes Anestesia

    2014-01-01

    Breast cancer is one of the causes of cancer death worldwide after lung cancer, stomach, liver and colon. One of medicinal plants traditionally used to treat cancer is bangun-bangun leaves (Plectranthus amboinicus, (Lour.) Spreng). The purpose of this study was to determine the ability of ethanolic leaves extract of bangun-bangun in the inhibition of mammary carcinogenesis induced by benzo(α)piren in female mice and to investigate the dose of ethanolic leaves extract of bangun-bangun that giv...

  16. Variação da quantidade de β-cariofileno em óleo essencial de Plectranthus amboinicus (Lour.) Spreng., Lamiaceae, sob diferentes condições de cultivo Variation in the amount of β-caryophyllene in essential oil of Plectranthus amboinicus (Lour.) Spreng. Lamiaceae under different conditions of cultivation

    OpenAIRE

    Fabíola B. Carneiro; Irinaldo D. Júnior; Pablo Q. Lopes; Macêdo, Rui O.

    2010-01-01

    O presente trabalho teve como objetivo avaliar a influência do adubo, da irrigação, da incidência solar, do horário de coleta e da idade da planta na quantidade de β-cariofileno no óleo essencial de Plectranthus amboinicus (Lour.) Spreng., Lamiaceae. As plantas foram cultivadas em canteiros experimentais entre os meses de dezembro de 2006 a setembro de 2007. O substrato utilizado foi adubo orgânico (esterco bovino), adubo mineral 1 [NPK (nitrogênio, fósforo e potássio)] e adubo mineral 2...

  17. In vivo antimalarial activity of leaves of Plectranthus amboinicus (lour) spreng on Plasmodium berghei yoelii.

    Science.gov (United States)

    Periyanayagam, K; Nirmala Devi, K; Suseela, L; Uma, A; Ismail, M

    2008-06-01

    An invivo study of aqueous extract of the leaves of Plectranthus amboinicus on Plasmodium berghei yoelii was conducted on laboratory infected albino mice and compared with standard drug chloroquine. Reduction of parasitemia at 250 mg/kg and 500 mg/kg of aqueous extract for 24 hrs, 48 hrs, 72 hrs and 96 hrs were determined. The reduction of parasitemia after 96 hrs was 100%, 67.9% and 76.2% for standard, 250 mg/kg and 500 mg/kg of aqueous extract respectively. The isolation of active principle responsible for the reduction of parasitemia may give a promising drug molecule. PMID:19301696

  18. Effect of subinihibitory and inhibitory concentrations of Plectranthus amboinicus (Lour.) Spreng essential oil on Klebsiella pneumoniae.

    Science.gov (United States)

    Gonçalves, Thially Braga; Braga, Milena Aguiar; de Oliveira, Francisco F M; Santiago, Gilvandete M P; Carvalho, Cibele B M; Brito e Cabral, Paula; de Melo Santiago, Thiago; Sousa, Jeanlex S; Barros, Eduardo Bedê; do Nascimento, Ronaldo Ferreira; Nagao-Dias, Aparecida T

    2012-08-15

    We evaluated the antimicrobial activity and some mechanisms used by subinhibitory and inhibitory concentrations of the essential oil, obtained from leaves of Plectranthus amboinicus, against a standard strain of Klebsiella pneumoniae and 5 multiresistant clinical isolates of the bacteria. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC), the rate of kill and the pH sensitivity of the essential oil were determined by microdilution tests performed in 96-well plates. Subinhibitory and inhibitory concentrations of the essential oil were tested in order to check its action on K. pneumoniae membrane permeability, capsule expression, urease activity and cell morphology. The MIC and MBC of the essential oil were 0.09±0.01%. A complete inhibition of the bacterial growth was observed after 2 h of incubation with twice the MIC of the essential oil. A better MIC was found when neutral or alkaline pH broth was used. Alteration in membrane permeability was found by the increase of crystal violet uptake when the bacteria were incubated with twice the MIC levels of the essential oil. The urease activity could be prevented when all the subinhibitory concentrations were tested in comparison to the untreated group (pamboinicus can be a good candidate for future research. PMID:22776104

  19. Variação da quantidade de β-cariofileno em óleo essencial de Plectranthus amboinicus (Lour. Spreng., Lamiaceae, sob diferentes condições de cultivo Variation in the amount of β-caryophyllene in essential oil of Plectranthus amboinicus (Lour. Spreng. Lamiaceae under different conditions of cultivation

    Directory of Open Access Journals (Sweden)

    Fabíola B. Carneiro

    2010-09-01

    Full Text Available O presente trabalho teve como objetivo avaliar a influência do adubo, da irrigação, da incidência solar, do horário de coleta e da idade da planta na quantidade de β-cariofileno no óleo essencial de Plectranthus amboinicus (Lour. Spreng., Lamiaceae. As plantas foram cultivadas em canteiros experimentais entre os meses de dezembro de 2006 a setembro de 2007. O substrato utilizado foi adubo orgânico (esterco bovino, adubo mineral 1 [NPK (nitrogênio, fósforo e potássio] e adubo mineral 2 (NPK com calcário sob diferentes tratamentos. A técnica analítica quantitativa utilizada foi a cromatografia gasosa (GC/FID. De acordo com os resultados obtidos nos meses de menor precipitação de chuvas obteve-se maior rendimento de óleo essencial, e os meses de maior precipitação de chuvas mostraram uma tendência de baixos rendimentos.This study aimed to evaluate the influence of fertilizer, irrigation, the incidence sun, the time of collection and the plant in the amount of β-caryophyllene in the essential oil of Plectranthus amboinicus (Lour. Spreng., Lamiaceae. The plants were grown in experimental beds between the months of December 2006 to September 2007. The substrate was used organic fertilizer (esterco veal, fertilizer mineral 1 [NPK (nitrogen, phosphorus and potassium] and fertilizer mineral 2 (NPK with limestone under different treatments. The quantitative analytical technique was used to gas chromatography (GC/FID. According to the results obtained in months of lower precipitation of rainfall received are higher yield of essential oil, and the months of highest precipitation of rain showed a trend of low income.

  20. In Vitro and In Vivo Leishmanicidal Activity of Astronium fraxinifolium (Schott) and Plectranthus amboinicus (Lour.) Spreng against Leishmania (Viannia) braziliensis

    OpenAIRE

    Silvio César Gomes de Lima; Maria Jania Teixeira; José Evaldo Gonçalves Lopes Júnior; Selene Maia de Morais; Alba Fabiola Torres; Milena Aguiar Braga; Raphael Oliveira Rodrigues; Gilvandete Maria Pinheiro Santiago; Alice Costa Martins; Aparecida Tiemi Nagao-Dias

    2014-01-01

    The aim of the present work was to evaluate antileishmanial activity of Astronium fraxinifolium and Plectranthus amboinicus. For the in vitro tests, essential oil of P. amboinicus (OEPA) and ethanolic extracts from A. fraxinifolium (EEAF) were incubated with 106  promastigotes of L. (Viannia) braziliensis. The OEPA was able to reduce the parasite growth after 48 h; nonetheless, all the EEAFs could totally abolish the parasite growth. For the in vivo studies, BALB/c mice were infected subcutan...

  1. In vitro and in vivo leishmanicidal activity of Astronium fraxinifolium (Schott) and Plectranthus amboinicus (Lour.) Spreng against Leishmania (Viannia) braziliensis.

    Science.gov (United States)

    de Lima, Silvio César Gomes; Teixeira, Maria Jania; Lopes, José Evaldo Gonçalves; de Morais, Selene Maia; Torres, Alba Fabiola; Braga, Milena Aguiar; Rodrigues, Raphael Oliveira; Santiago, Gilvandete Maria Pinheiro; Martins, Alice Costa; Nagao-Dias, Aparecida Tiemi

    2014-01-01

    The aim of the present work was to evaluate antileishmanial activity of Astronium fraxinifolium and Plectranthus amboinicus. For the in vitro tests, essential oil of P. amboinicus (OEPA) and ethanolic extracts from A. fraxinifolium (EEAF) were incubated with 10(6) promastigotes of L. (Viannia) braziliensis. The OEPA was able to reduce the parasite growth after 48 h; nonetheless, all the EEAFs could totally abolish the parasite growth. For the in vivo studies, BALB/c mice were infected subcutaneously (s.c.) with 10(7) L. braziliensis promastigotes. Treatment was done by administering OEPA intralesionally (i.l.) for 14 days. No difference was found in lesion thickness when those animals were compared with the untreated animals. Further, golden hamsters were infected s.c. with 10(6) L. braziliensis promastigotes. The first protocol of treatment consisted of ethanolic leaf extract from A. fraxinifolium (ELEAF) administered i.l. for 4 days and a booster dose at the 7th day. The animals showed a significant reduction of lesion thickness in the 6th week, but it was not comparable to the animals treated with Glucantime. The second protocol consisted of 15 daily intralesional injections. The profiles of lesion thickness were similar to the standard treatment. In conclusion, in vivo studies showed a high efficacy when the infected animals were intralesionally treated with leaf ethanolic extract from A. fraxinifolium. PMID:24829921

  2. In Vitro and In Vivo Leishmanicidal Activity of Astronium fraxinifolium (Schott and Plectranthus amboinicus (Lour. Spreng against Leishmania (Viannia braziliensis

    Directory of Open Access Journals (Sweden)

    Silvio César Gomes de Lima

    2014-01-01

    Full Text Available The aim of the present work was to evaluate antileishmanial activity of Astronium fraxinifolium and Plectranthus amboinicus. For the in vitro tests, essential oil of P. amboinicus (OEPA and ethanolic extracts from A. fraxinifolium (EEAF were incubated with 106  promastigotes of L. (Viannia braziliensis. The OEPA was able to reduce the parasite growth after 48 h; nonetheless, all the EEAFs could totally abolish the parasite growth. For the in vivo studies, BALB/c mice were infected subcutaneously (s.c. with 107  L. braziliensis promastigotes. Treatment was done by administering OEPA intralesionally (i.l. for 14 days. No difference was found in lesion thickness when those animals were compared with the untreated animals. Further, golden hamsters were infected s.c. with 106  L. braziliensis promastigotes. The first protocol of treatment consisted of ethanolic leaf extract from A. fraxinifolium (ELEAF administered i.l. for 4 days and a booster dose at the 7th day. The animals showed a significant reduction of lesion thickness in the 6th week, but it was not comparable to the animals treated with Glucantime. The second protocol consisted of 15 daily intralesional injections. The profiles of lesion thickness were similar to the standard treatment. In conclusion, in vivo studies showed a high efficacy when the infected animals were intralesionally treated with leaf ethanolic extract from A. fraxinifolium.

  3. Chemical composition and larvicidal activity of the essential oil of Plectranthus amboinicus (Lour.) Spreng against Anopheles stephensi: a malarial vector mosquito.

    Science.gov (United States)

    Senthilkumar, Annadurai; Venkatesalu, Venugopalan

    2010-10-01

    Essential oil of Plectranthus amboinicus was studied for its chemical composition and larvicidal potential against the malarial vector mosquito Anopheles stephensi. Totally 26 compounds were identified by GC and GC-MS. The major chemical compounds were carvacrol (28.65%) followed by thymol (21.66%), α-humulene (9.67%), undecanal (8.29%), γ-terpinene (7.76%), ρ-cymene (6.46%), caryophyllene oxide (5.85%), α-terpineol (3.28%) and β-selinene (2.01%). The larvicidal assay was conducted to record the LC(50) and LC(90) values and the larval mortality was observed after 12 and 24 h of exposure period. The LC(50) values of the oil were 33.54 (after 12 h) and 28.37 ppm (after 24 h). The LC(90) values of the oil were 70.27 (after 12 h) and 59.38 ppm (after 24 h). The results of the present study showed that the essential oil of P. amboinicus is one of the inexpensive and eco-friendly sources of natural mosquito larvicidal agent to control/reduce the population of malarial vector mosquito. PMID:20668876

  4. Indole acetic acid production by fluorescent Pseudomonas spp. from the rhizosphere of Plectranthus amboinicus (Lour.) Spreng. and their variation in extragenic repetitive DNA sequences.

    Science.gov (United States)

    Sethia, Bedhya; Mustafa, Mariam; Manohar, Sneha; Patil, Savita V; Jayamohan, Nellickal Subramanian; Kumudini, Belur Satyan

    2015-06-01

    Fluorescent Pseudomonas (FP) is a heterogenous group of growth promoting rhizobacteria that regulate plant growth by releasing secondary metabolic compounds viz., indole acetic acid (IAA), siderophores, ammonia and hydrogen cyanide. In the present study, IAA producing FPs from the rhizosphere of Plectranthus amboinicus were characterized morphologically, biochemically and at the molecular level. Molecular identification of the isolates were carried out using Pseudomonas specific primers. The effect of varying time (24, 48, 72 and 96 h), Trp concentrations (100, 200, 300, 400 and 500 μg x ml(-1)), temperature (10, 26, 37 and 50 ± 2 degrees C) and pH (6, 7 and 8) on IAA production by 10 best isolates were studied. Results showed higher IAA production at 72 h incubation, at 300 μg x ml(-1) Trp concentration, temperature 26 ± 2 degrees C and pH 7. TLC with acidified ethyl acetate extract showed that the IAA produced has a similar Rf value to that of the standard IAA. Results of TLC were confirmed by HPLC analysis. Genetic diversity of the isolates was also studied using 40 RAPD and 4 Rep primers. Genetic diversity parameters such as dominance, Shannon index and Simpson index were calculated. Out of 40 RAPD primers tested, 9 (2 OP-D series and 7 OP-E series) were shortlisted for further analysis. Studies using RAPD, ERIC, BOX, REP and GTG5 primers revealed that isolates exhibit significant diversity in repetitive DNA sequences irrespective of the rhizosphere. PMID:26155673

  5. Interference of Plectranthus amboinicus (Lour.) Spreng essential oil on the anti-Candida activity of some clinically used antifungals Interferência do óleo essencial de Plectranthus amboinicus (Lour.) Spreng sobre a atividade anti-Candida de alguns antifúngicos utilizados clinicamente

    OpenAIRE

    Rinalda de Araújo G. Oliveira; EDELTRUDES O. LIMA; Souza, Evandro L.; Wellington L. Vieira; Kristerson R. L. Freire; Vinícius N. Trajano; Igara O. Lima; Raimundo N. Silva-Filho

    2007-01-01

    Plants with medicinal properties have been applied for a long in the traditional health care, so that sometimes their use takes place concomitantly to the use of industrialized drugs. The aim of this study was to evaluate the interference of Plectranthus amboinicus essential oil on the anti-Candida activity of some clinically used antifungals by the solid medium diffusion procedure. Assayed antifungals were amphotericin B (100 µg/mL), ketoconazole (50 µg/mL) and itraconazole (50 &...

  6. Interference of Plectranthus amboinicus (Lour. Spreng essential oil on the anti-Candida activity of some clinically used antifungals Interferência do óleo essencial de Plectranthus amboinicus (Lour. Spreng sobre a atividade anti-Candida de alguns antifúngicos utilizados clinicamente

    Directory of Open Access Journals (Sweden)

    Rinalda de Araújo G. de Oliveira

    2007-06-01

    Full Text Available Plants with medicinal properties have been applied for a long in the traditional health care, so that sometimes their use takes place concomitantly to the use of industrialized drugs. The aim of this study was to evaluate the interference of Plectranthus amboinicus essential oil on the anti-Candida activity of some clinically used antifungals by the solid medium diffusion procedure. Assayed antifungals were amphotericin B (100 µg/mL, ketoconazole (50 µg/mL and itraconazole (50 µg/mL. C. albicans, C. tropicalis, C. guilliermondii, C. krusei and C. stellatoidea were used as test microorganisms. P. amboinicus essential oil showed MIC value for most assayed yeast strains. The essential oil when assayed in its MIC value showed some interference on the anti-Candida effectiveness of the assayed antifungals. It was noted yeast growth inhibition zones with different diameters when the antifungals were tested alone and combined with the essential oil. P. amboinicus essential oil showed prominent interference on the anti-yeast activity of itraconazole providing a synergic effect on C. albicans, C. tropicalis, C. krusei and C. stellatoidea. Also, the essential oil interfered on the anti-yeast activity of ketoconazole when interacting with C. albicans, C. guilliermondii and C. stellatoidea providing, respectively, an antagonic and synergic effect. On the other hand, it was found a small interference on the anti-yeast effect of amphotericin B. These data showed that combined use of medicinal plants and/or derivatives with industrialized drugs, particularly antimicrobials, could interfere on their expected therapeutic effects.As plantas com propriedades medicinais têm sido utilizadas por um longo tempo no cuidado tradicional de saúde, de modo que algumas vezes seu uso ocorre concomitantemente ao uso de drogas industrializadas. O objetivo deste estudo foi avaliar a interferência do óleo essencial de Plectranthus amboinicus sobre atividade anti-Candida de

  7. Uji Sitotoksik Fraksi Etilasetat Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) terhadap Larva Artemia salina Leach dengan Metode Brine Shrimp Lethality Test (BSLT)

    OpenAIRE

    Pardede, E. Roslina

    2016-01-01

    Background: Bangun-Bangun leaves (Plectranthus amboinicus (Lour.) Spreng is one of the plants that have the potential to be developed as a cancer chemopreventive agent. The Purpose: The aim of the study were to find out the cytotoxic effect of ethylacetate fraction bangun-bangun leaves (FEDBB) on Artemia salina Leach larvae. Methods: This experimental study using 800 Artemia salina Leach larvae were divided into one control group negative 1 and 5 positive control group concentration10 μ...

  8. Estudo anatômico comparado de órgãos vegetativos de boldo miúdo, Plectranthus ornatus Codd. e malvariço, Plectranthus amboinicus (Lour. Spreng. - Lamiaceae

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    Claudia Mauro

    2008-12-01

    Full Text Available Foi feita uma investigação anatômica dos órgãos vegetativos de Plectranthus ornatus, "boldo miúdo" e Plectranthus amboinicus, "malvariço", da família Lamiaceae. Foram estudados o limbo foliar, pecíolo e caule destas duas espécies. Ambas apresentam limbo foliar com tricomas tectores, com tricomas glandulares pedicelados em P. amboinicus e pedicelados e sésseis em P. ornatus. Justifica-se esta investigação, pelo fato de se tratar de espécies utilizadas, popularmente, como fitoterápico.

  9. Estudo anatômico comparado de órgãos vegetativos de boldo miúdo, Plectranthus ornatus Codd. e malvariço, Plectranthus amboinicus (Lour.) Spreng. - Lamiaceae

    OpenAIRE

    Claudia Mauro; Celi de Paula Silva; Juliana Missima; Thiana Ohnuki; Renata B. Rinaldi; Melissa Frota

    2008-01-01

    Foi feita uma investigação anatômica dos órgãos vegetativos de Plectranthus ornatus, "boldo miúdo" e Plectranthus amboinicus, "malvariço", da família Lamiaceae. Foram estudados o limbo foliar, pecíolo e caule destas duas espécies. Ambas apresentam limbo foliar com tricomas tectores, com tricomas glandulares pedicelados em P. amboinicus e pedicelados e sésseis em P. ornatus. Justifica-se esta investigação, pelo fato de se tratar de espécies utilizadas, popularmente, como fitoterápico....

  10. The chemotherapeutic effect of essential oil of Plectranthus amboinicus (Lour) on lung metastasis developed by B16F-10 cell line in C57BL/6 mice.

    Science.gov (United States)

    Manjamalai, A; Grace, V M Berlin

    2013-01-01

    Current investigation is to evaluate the anticancer activity of the essential oil of Plectranthus amboinicus (Lour) on B16F-10 melanoma cell line injected C57BL/6 mice, and it was simultaneously treated with the essential oil of P. amboinicus (Lour) (50 μg/dose) via i.p. for 21 days. The present investigation exhibited the potent chemotherapeutic/chemopreventive effect of the essential oil of P. amboinicus (Lour) over lung metastasis that developed. To our knowledge, this is the first report in evaluating the effect of essential oil of P. amboinicus (Lour) using lung cancer model. PMID:23249189

  11. The transverse strength of acrylic resin after Coleus amboinicus, Lour extract solution immersion

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    Devi Rianti

    2006-12-01

    Full Text Available A laboratoric experimental study was conducted on the transverse strength of acrylic resin after Coleus amboinicus, Lour extract solution immersion. The aim of this study is to know the difference of acrylic resin transverse strengths caused by immersion time variations in a concentrate solution. The study was carried out on unpolished acrylic resin plates with 65 × 10 × 2,5 mm dimension; solution with 15% Coleus amboinicus, Lour extract, and 30, 60, 90 days immersion times to measure the transverse strength and sterilized aquadest was used as control. Acrylic resin plates transverse strength was measured using Autograph AG-10 TE. The data was analyzed using One-Way Anova and LSD with 5% degree of significance. The result showed that longer immersion time will decrease the transverse strength of the acrylic resin plates. After 90 days immersion time, the transverse strength decrease is still above the recommended standard transverse strength.

  12. FORMULASI MINUMAN SUPLEMEN DAUN TORBANGUN (Coleus amboinicus Lour.) UNTUK WANITA YANG MENDERITA PMS (PREMENSTRUAL SYNDROME)

    OpenAIRE

    Pramadya Alfitra; Budi Setiawan; M. Rizal Damanik

    2012-01-01

    Torbangun (Coleus amboinicus Lour) is one of Labiatae family containing a lot of micronutrients and active components. The plant is used as traditional medicine for relieving premenstrual syndromes (PMS). Based on the results of previous studies, a capsule containing 750 mg of dried Torbangun leaves can relieve PMS as well as commercial herbal, and better than placebo. Capsule supplement form seems less acceptable because it tastes like taking a drug. Therefore, it requires development for ...

  13. PERUBAHAN MUTU DAN UMUR SIMPAN SUP DAUN TORBANGUN (Colues amboinicus Lour DALAM KEMASAN

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    Endang Warsiki

    2012-11-01

    Full Text Available ABSTRACTTorbangun leaves soup is one of the traditional diets commonly consumed by lactating mothers among the Batak society. The soup is believed to increase breast milk production and helps recovery process after the mother giving birth. Unfortunately, until now the soup is only served directly after cooking thereby limits its usefulness. Given the potential of the soup is quite important, the preservation of the cooked soup needs to be done so that the soup can be consumed at anytimeany time. This study aims to determine the shelf life of torbangun soup in a variety of packaging and at various storage temperatures. The soup is stored at three different temperatures (3-5oC, 10-12oC and 27-30oC and in 3 types of packaging namely polyethylene terephthalate (PET, polypropylene (PP and cans. Changes in product quality during storage are analyzed and estimated the shelf life of products. The results show that the soup stored at the 3-5oC and 10-12oC has longer shelf life compared to 27-30oC (day 8 vs day 3. In addition, the cans are the best packing among other packaging materials.Key words: torbangun leaf, Coleus amboinicus Lour, soup, shelf lifeABSTRAKSup daun Torbangun torbangun adalah salah satu makanan tradisional yang biasa dikonsumsi oleh ibu menyusuidi kalangan masyarakat Batak. Sup ini diyakini dapat meningkatkan produksi ASI dan membantu proses pemulihan setelah ibu melahirkan. Sayangnya, sampai saat ini sup daun torbangun hanya disajikan langsung setelah dimasak sehingga membatasi kegunaannya. Mengingat potensi sup ini cukup penting, penggunaan bahan pengawet pada sup matang perlu dilakukan agar sup ini dapat dikonsumsi kapan saja. Penelitian ini bertujuan untuk menentukan umur simpan sup torbangun dalam berbagai kemasan dan pada berbagai suhu penyimpanan. Pada penelitian ini sup disimpan pada tiga suhu berbeda (3-5oC, 10-12oC dan 27-30oC dan di simpan dalam tiga jenis kemasan yaitu polietilen tereftalat (PET, polypropylene (PP dan

  14. FORMULASI MINUMAN SUPLEMEN DAUN TORBANGUN (Coleus amboinicus Lour. UNTUK WANITA YANG MENDERITA PMS (PREMENSTRUAL SYNDROME

    Directory of Open Access Journals (Sweden)

    Pramadya Alfitra

    2012-03-01

    Full Text Available 800x600 Normal 0 false false false IN X-NONE X-NONE MicrosoftInternetExplorer4 Torbangun (Coleus amboinicus Lour is one of Labiatae family containing a lot of micronutrients and active components. The plant is used as traditional medicine for relieving premenstrual syndromes (PMS. Based on the results of previous studies, a capsule containing 750 mg of dried Torbangun leaves can relieve PMS as well as commercial herbal, and better than placebo. Capsule supplement form seems less acceptable because it tastes like taking a drug. Therefore, it requires development for Torbangun supplement product in other forms, and one of them is a supplement drink. The study is aimed to formulate  a supplement drink from Torbangun leaves as the basic ingredients. Supplement drink formulation is made in trial and error by combining extract of torbangun leaves, sucrose, and lemon, obtaining three formulas: DT1 contains 10 g extract of torbangun leaves and 16 g sucrose, DT2 contains 10 g extract of torbangun leaves, 2 g lemon, 22 g sucrose, and DT3 contains 10 g extract of torbangun leaves, 4 g lemon, and 28 g sucrose. These formulas are then tested by organoleptic, physical, and chemical. The results show that the formula DT3 has the highest average score. Blanching treatment improves organoleptic properties of supplement drinks. Mean scores in color, aroma, and taste of the blanched supplement drink are different (p Key words: Torbangun leaves, premenstrual syndrome, supplement drink, blanching.

  15. БИОМОРФОЛОГИЧЕСКИЕ ОСОБЕННОСТИ ШПОРОЦВЕТНИКА АМБОИНСКОГО (PLECTRANTHUS AMBOINICUS (LOUR. J SPRENG.) В ЗАВИСИМОСТИ ОТ УРОВНЯ ОСВЕЩЕННОСТИ И ДОЗ УДОБРЕНИЙ

    OpenAIRE

    Цицилин, А.; Мотина, Е.

    2011-01-01

    Описаны основные биоморфологические параметры растений Plectranthus amboinicus (Lour.) Spreng., растущих при трех уровнях освещенности100, 500, 2000 люкс и разных дозах удобрений. Растения образуют наибольшую листовую поверхность, имеют высокие декоративные качества и содержат максимальное количество эфирного масла при относительно высоком уровне освещенности 2000 люкс. При освещенности 500 люкс наблюдается достоверное увеличение площади листьев почти на 55% при использовании минеральных удоб...

  16. Torbangun (Coleus amboinicus Lour): a Bataknese traditional cuisine perceived as lactagogue by Bataknese lactating women in Simalungun, North Sumatera, Indonesia.

    Science.gov (United States)

    Damanik, Rizal

    2009-02-01

    Torbangun (Coleus amboinicus Lour) has been used as a breast milk stimulant (a lactagogue) by Bataknese people in Indonesia for hundreds of years. However, the traditional use of torbangun is not well documented, and scientific evidence is limited to establish coleus as a lactagogue. This Focus Group Discussion (FGD) study was conducted to gather information regarding the practice and cultural beliefs related to the traditional use of torbangun as a lactagogue. The main findings of this investigation were: (1) torbangun, which is considered nourishing, is usually given to the mother for one month after giving birth in order to restore her state of balance; (2) in the Bataknese culture, torbangun is perceived to serve several purposes which include enhancing breast milk production and acting as a uterine cleansing agent; and (3) the tradition has been practiced for hundreds of years, and its adherence is still strong.

  17. Plectranthus amboinicus and Centella asiatica Cream for the Treatment of Diabetic Foot Ulcers

    OpenAIRE

    Yuan-Sung Kuo; Hsiung-Fei Chien; William Lu

    2012-01-01

    Effects of a topical cream containing P. amboinicus (Lour.) Spreng. (Lamiaceae) and C. asiatica (L.) Urban (Umbelliferae) were evaluated and compared to effects of hydrocolloid fiber wound dressing for diabetic foot ulcers. A single-center, randomized, controlled, open-label study was conducted. Twenty-four type 1 or type 2 diabetes patients aged 20 years or older with Wagner grade 3 foot ulcers postsurgical debridement were enrolled between October 2008 and December 2009. Twelve randomly ass...

  18. SUPLEMENTASI DAUN TORBANGUN (COLEUS AMBOINICUS LOUR UNTUK MENURUNKAN KELUHAN SINDROM PRAMENSTRUASI PADA REMAJA PUTRI (SUPPLEMENTATION OF TORBANGUN LEAVES [COLEUS AMBOINICUS LOUR] IN REDUCING THE COMPLAINST OF PRE-MENSTRUAL SYNDROME [PMS] AMONG TEENAGE G

    Directory of Open Access Journals (Sweden)

    Mazarina Devi

    2013-07-01

    Full Text Available ABSTRACT Background: Torbangun leaves (Coleus ambonicus Lour is one of type of species from Labiatae family which contains a lot of micronutrient and active element which have been examined as beneficial for human health and quality of life. The plants containing iridoid and flavonoid as well as phytochemical which deal with reproduction hormone is applicable to traditional medication toward PMS cases. Methods: The present study was aimed to assess the efficacy of CAL leaves in the management of PMS. An experimental clinical trial was conducted in 35 teenagers with PMS. Three intervention groups were defined: CAL leaves; commercial preparation; and placebo. Participants were followed-up individually for 1 month. Result: The results showed average menarche occurred around age 13 years, with menstruation lasting 5 days. During each successive treatment cycle, participants experienced a lower pain intensity score. Chi Square test, after adjusting each cycle for baseline pain, treatment compliance and other variables, showed that the group receiving CAL extract had significantly reduced pain intensity (p<0.05 compared with commercial preparation and placebo. Conclusion: The torbangun leaves supplement can be used as treatment to relief symptoms of the premenstrual syndrome. [Penel Gizi Makan 2010, 33(2:180-194]   Keywords: Torbangun leaves (Coleus amboinicus Lour, pre-menstrual syndrome (PMS, teenage girls

  19. Avaliação da atividade antimicrobiana e antioxidante das espécies Plectranthus amboinicus (Lour.) e Mentha x villosa (Huds.)

    OpenAIRE

    Rosangela Correia Freitas; Rhuanna Rackel de Sá Azevedo; Larissa Isabela Oliveira Souza; Thiago José Matos Rocha; Aldenir Feitosa dos Santos

    2014-01-01

    O uso de plantas medicinais com potencial terapêutico tem motivado a realização de estudos através de ensaios experimentais que visam fornecer informações úteis e de extrema importância. Assim o presente trabalho objetivou avaliar a atividade antimicrobiana e antioxidante das espécies de Coleus amboinicus (Lour.) e Mentha x villosa (Huds). A atividade antimicrobiana foi avaliada pelo método de difusão em disco, cepas bacterianas padronizadas Gram negativas Escherichia ...

  20. Ensaios clínicos toxicológicos, fase I, de um fitoterápico composto (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill) Phase I clinical toxicological assays of a complex herbal medicine (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour and Eucaliptus globulus Labill)

    OpenAIRE

    Patrícia Trindade C. Paulo; Margareth F. F. M. Diniz; Isac A. Medeiros; Liana C. S. L. Morais; Fábia Barbosa de Andrade; Hosana Bandeira Santos

    2009-01-01

    Neste estudo, foram realizados ensaios clínicos toxicológicos, fase I, do produto fitoterápico composto pelas plantas medicinais Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill. O estudo foi realizado no Hospital Universitário Lauro Wanderley/UFPB/PB e, para isto, foram selecionados 28 voluntários sadios, sendo 14 homens e 14 mulheres que ingeriram por via oral, ininterruptamente durante 8 semanas, 15 mL do produto, três vezes ao dia; e no 3º e 7º dia...

  1. Avaliação da atividade antimicrobiana e antioxidante das espécies Plectranthus amboinicus (Lour. e Mentha x villosa (Huds.

    Directory of Open Access Journals (Sweden)

    Rosangela Correia Freitas

    2014-01-01

    Full Text Available O uso de plantas medicinais com potencial terapêutico tem motivado a realização de estudos através de ensaios experimentais que visam fornecer informações úteis e de extrema importância. Assim o presente trabalho objetivou avaliar a atividade antimicrobiana e antioxidante das espécies de Coleus amboinicus (Lour. e Mentha x villosa (Huds. A atividade antimicrobiana foi avaliada pelo método de difusão em disco, cepas bacterianas padronizadas Gram negativas Escherichia coli (ATCC 25922 e ATCC 35218, cepas Gram positivas Staphylococcus aureus (ATCC 25923 e S. aureus ATCC 27853, assim como fungos padrões de Cryptococcus neoformans (pertencentes à coleção de microrganismos do CESMAC. Como controle positivo foi usado o imipenem e fluconazol e como controle negativo um disco com etanol absoluto. A determinação do potencial antioxidante foi realizada utilizando os métodos DPPH, FTC e determinação de compostos fenólicos. Os extratos etanólicos de C. amboinicus e M. x villosa apresentaram excelentes resultados tanto relacionado à atividade antimicrobiana quanto à antioxidante. Nossos resultados mostram a potencialidade das plantas analisadas na prevenção e no combate de doenças.

  2. SUPLEMENTASI DAUN TORBANGUN (COLEUS AMBOINICUS LOUR) UNTUK MENURUNKAN KELUHAN SINDROM PRAMENSTRUASI PADA REMAJA PUTRI (SUPPLEMENTATION OF TORBANGUN LEAVES [COLEUS AMBOINICUS LOUR] IN REDUCING THE COMPLAINST OF PRE-MENSTRUAL SYNDROME [PMS] AMONG TEENAGE G

    OpenAIRE

    Mazarina Devi; Hidayat Syarief; Rizal Damanik; Ahmad Sulaeman; Budi Setiawan; Rousmala Dewi

    2013-01-01

    ABSTRACT Background: Torbangun leaves (Coleus ambonicus Lour) is one of type of species from Labiatae family which contains a lot of micronutrient and active element which have been examined as beneficial for human health and quality of life. The plants containing iridoid and flavonoid as well as phytochemical which deal with reproduction hormone is applicable to traditional medication toward PMS cases. Methods: The present study was aimed to assess the efficacy of CAL leaves in the managemen...

  3. Karakteristik Pertumbuhan Vegetatif, Kandungan Sterol dan Klorofil dari Beberapa Aksesi Tanaman Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng)

    OpenAIRE

    Nasution, Nuraminah

    2016-01-01

    Research was purposed to find the vegetative growth characteristic, sterols and chlorophyll content of some accessions indian borage. This research was held at home screen Agriculture Faculty, University of Sumatera Utara, Medan was began from December 2015 until February 2016 with accession Medan (Krakatau), Medan (Tuntungan), Sibolangit, Simalungun and Brastagi. The results showed that morphology character of accession plants Medan (Tuntungan), Sibolangit, Simalungun and Brastagi not signif...

  4. Ensaios clínicos toxicológicos, fase I, de um fitoterápico composto (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill Phase I clinical toxicological assays of a complex herbal medicine (Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour and Eucaliptus globulus Labill

    Directory of Open Access Journals (Sweden)

    Patrícia Trindade C. Paulo

    2009-03-01

    Full Text Available Neste estudo, foram realizados ensaios clínicos toxicológicos, fase I, do produto fitoterápico composto pelas plantas medicinais Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour e Eucalyptus globulus Labill. O estudo foi realizado no Hospital Universitário Lauro Wanderley/UFPB/PB e, para isto, foram selecionados 28 voluntários sadios, sendo 14 homens e 14 mulheres que ingeriram por via oral, ininterruptamente durante 8 semanas, 15 mL do produto, três vezes ao dia; e no 3º e 7º dia, 3ª e 6ª semanas e 24 h após a 8ª semana, foram feitas avaliações clínicas e laboratoriais para análise da toxicidade aguda e crônica. Os resultados obtidos demonstraram que os pacientes não apresentaram alterações clínicas, laboratoriais e reações adversas significantes, apenas pequenas alterações foram detectadas no sangue através da aspartato transaminase (AST e fosfatase alcalina no grupo feminino para um p In this study, phase I clinical toxicological assays of the herbal medicine composed of the medicinal plants Schinus terebinthifolius Raddi, Plectranthus amboinicus Lour and Eucaliptus globulus Labill were performed. The study was carried out at Hospital Universitário Lauro Wanderley/UFPB/PB/Brazil and for this purpose, 28 healthy volunteers were chosen, 14 men and 14 women who ingested 15 mL of the medicine per oral, with no interruption, three times a day; and on the 3rd and 7th days, on the 3rd and 6th weeks and 24h after the 8th week, clinical and laboratory evaluations were performed to analyze the acute and chronic toxicity. As results, the patients did not show significant clinical and laboratory alterations and adverse reactions, only little alterations were detected in blood through aspartate transaminase (AST and alkaline phosphatase in the female group to a p < 0.05; however, these values are according to the normality standard for adult individuals. It can be concluded that these data, complementary to those

  5. Plectranthus amboinicus and Centella asiatica Cream for the Treatment of Diabetic Foot Ulcers.

    Science.gov (United States)

    Kuo, Yuan-Sung; Chien, Hsiung-Fei; Lu, William

    2012-01-01

    Effects of a topical cream containing P. amboinicus (Lour.) Spreng. (Lamiaceae) and C. asiatica (L.) Urban (Umbelliferae) were evaluated and compared to effects of hydrocolloid fiber wound dressing for diabetic foot ulcers. A single-center, randomized, controlled, open-label study was conducted. Twenty-four type 1 or type 2 diabetes patients aged 20 years or older with Wagner grade 3 foot ulcers postsurgical debridement were enrolled between October 2008 and December 2009. Twelve randomly assigned patients were treated with WH-1 cream containing P. amboinicus and C. asiatica twice daily for two weeks. Another 12 patients were treated with hydrocolloid fiber dressings changed at 7 days or when clinically indicated. Wound condition and safety were assessed at days 7 and 14 and results were compared between groups. No statistically significant differences were seen in percent changes in wound size at 7- and 14-day assessments of WH-1 cream and hydrocolloid dressing groups. A slightly higher proportion of patients in the WH-1 cream group (10 of 12; 90.9%) showed Wagner grade improvement compared to the hydrocolloid fiber dressing group but without statistical significance. For treating diabetic foot ulcers, P. amboinicus and C. asiatica cream is a safe alternative to hydrocolloid fiber dressing without significant difference in effectiveness. PMID:22693530

  6. Plectranthus amboinicus and Centella asiatica Cream for the Treatment of Diabetic Foot Ulcers

    Directory of Open Access Journals (Sweden)

    Yuan-Sung Kuo

    2012-01-01

    Full Text Available Effects of a topical cream containing P. amboinicus (Lour. Spreng. (Lamiaceae and C. asiatica (L. Urban (Umbelliferae were evaluated and compared to effects of hydrocolloid fiber wound dressing for diabetic foot ulcers. A single-center, randomized, controlled, open-label study was conducted. Twenty-four type 1 or type 2 diabetes patients aged 20 years or older with Wagner grade 3 foot ulcers postsurgical debridement were enrolled between October 2008 and December 2009. Twelve randomly assigned patients were treated with WH-1 cream containing P. amboinicus and C. asiatica twice daily for two weeks. Another 12 patients were treated with hydrocolloid fiber dressings changed at 7 days or when clinically indicated. Wound condition and safety were assessed at days 7 and 14 and results were compared between groups. No statistically significant differences were seen in percent changes in wound size at 7- and 14-day assessments of WH-1 cream and hydrocolloid dressing groups. A slightly higher proportion of patients in the WH-1 cream group (10 of 12; 90.9% showed Wagner grade improvement compared to the hydrocolloid fiber dressing group but without statistical significance. For treating diabetic foot ulcers, P. amboinicus and C. asiatica cream is a safe alternative to hydrocolloid fiber dressing without significant difference in effectiveness.

  7. Efek Imunomodulator Ekstrak Etanol Daun Bangun-Bangun (Plectranthus amboinicus (Lour.) Spreng) Terhadap Respon Hipersensitivitas Tipe Lambat Dan Titer Antibodi Sel Imun Mencit Jantan

    OpenAIRE

    Novika, Rita

    2015-01-01

    The immune system is useful to maintain the condition of the body against pathogenic microorganisms. When the immune system is not able to work well, improving the immune system becomes very important to keep the immune system still works optimally to face microorganisms attack. Bangun-bangun leaves is one of the plants that have the ability to enhance the body's defense. The objectives of this study was to know the immunomodulatory effect of ethanol extract of bangun-bangun leaves on delayed...

  8. Efek Antimutagenik Ekstrak Etil Asetat Daun Bangun-bangun (Plectranthus amboinicus, (Lour.) Spreng.) dan Gambaran Histopatologi Hati dan Ginjal Mencit yang Diinduksi Siklofosfamid

    OpenAIRE

    Hasibuan, Poppy Anjelisa Z.; Chrestella, Jessy

    2016-01-01

    Latar belakang: Pengobatan kanker yang aman masih belum ditemukan karena obat kanker yang ideal seharusnya dapat menghabiskan sel kanker tanpa membahayakan jaringan sehat, oleh karena itu perlu terus dilakukan upaya menggali sumber alam nabati yang secara empiris telah banyak digunakan masyarakat untuk mengobati kanker. Daun bangun-bangun mempunyai aktifitas antioksidan yang tinggi, memiliki efek sebagai anticlastogenic dan berpotensi sebagai radioprotektif, mampu melindungi te...

  9. Pengaruh Daun Bangun Bangun (coleus amboinicus) Untuk Meningkatkan Produksi ASI

    OpenAIRE

    Idris, Rahmi

    2014-01-01

    Latar belakang : Daun Bangun-bangun atau Torbangun (Colues amboinicus Lour) adalah salah satu jenis tanaman yang umum dikonsumsi oleh ibu yang baru melahirkan di daerah Sumatera Utara, khususnya oleh suku batak. Daun Bangun-bangun dipercaya dapat meningkatkan produksi air susu ibu (ASI). Daun Bangun-bangun ini memiliki kandungan zat gizi tinggi, terutama zat besi dan karoten. Ditemukan pula bahwa konsumsi daun Bangun-bangun berpengaruh nyata terhadap peningkatan kadar beberapa mineral seperti...

  10. PATHORCHUR (COLEUS AROMATICUS): A REVIEW OF THE MEDICINAL EVIDENCE FOR ITS PHYTOCHEMISTRY AND PHARMACOLOGY PROPERTIES

    OpenAIRE

    Om Prakash Rout; Rabinarayan Acharya; Sagar Kumar Mishra; Rashmibala Sahoo

    2012-01-01

    Coleus aromaticus Benth., (Fam. Lamiaceae), syn. Coleus amboinicus Lour. Spreng or Plectranthus ambonicus Lour, is commonly known as Indian/ country borage and ‘Pathorchur’ in Hindi and Bengali. It is recorded in the Indian system of medicine as one of the sources of Pashanabheda. It is large succulent aromatic perennial herb, shrubby below, hispidly villous or tomentose. It is found throughout India, Ceylon and Moluccas. The leaves of the plant are bitter, acrid and were being widely used tr...

  11. PERUBAHAN MUTU DAN UMUR SIMPAN SUP DAUN TORBANGUN (Colues amboinicus Lour) DALAM KEMASAN

    OpenAIRE

    Endang Warsiki; M. Rizal Damanik

    2012-01-01

    ABSTRACTTorbangun leaves soup is one of the traditional diets commonly consumed by lactating mothers among the Batak society. The soup is believed to increase breast milk production and helps recovery process after the mother giving birth. Unfortunately, until now the soup is only served directly after cooking thereby limits its usefulness. Given the potential of the soup is quite important, the preservation of the cooked soup needs to be done so that the soup can be consumed at anytimeany ti...

  12. Antimalarial activity of Malaysian Plectranthus amboinicus against Plasmodium berghei

    OpenAIRE

    Ramli, Norazsida; Ahamed, Pakeer Oothuman Syed; Elhady, Hassan Mohamed; Taher, Muhammad

    2014-01-01

    Context: Malaria is a mosquito-borne disease caused by parasitic protozoa from the genus of Plasmodium. The protozoans have developed resistance against many of current drugs. It is urgent to find an alternative source of new antimalarial agent. In the effort to discover new antimalarial agents, this research has been conducted on Plectranthus amboinicus. Aims: This study was conducted to evaluate the toxicity and antiplasmodial properties of P. amboinicus. Materials and Methods: Acute oral t...

  13. EVALUATION OF PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF PLECTRANTHUS AMBOINICUS

    OpenAIRE

    Arunkumar Sathasivam; Karthikeyan Elangovan

    2011-01-01

    Plectranthus amboinicus is a commonly available medicinal herb in India. The phytochemical active compounds qualitatively analyzed such as alkaloids, Terpenoids, Cardiac glycosides, saponin, tannins and flavonoids present in Plectranthus amboinicus. The aqueous, acetone and methanol crude plant extracts were prepared and tested against gram positive and gram negative bacteria. The highest antibacterial activities were observed in methanol extract (11± 0.19, 16± 0.45, 15±0.57, 17±0.23, 16± 0....

  14. Allergic contact dermatitis to Plectranthus amboinicus masquerading as chronic leg ulcer.

    Science.gov (United States)

    Chang, Shyue-Luen; Chang, Ya-Ching; Yang, Chin-Hsun; Hong, Hong-Shang

    2005-12-01

    This report discusses a case of a 69-year-old woman who developed chronic non-healing leg ulcers after long-term topical use of Plectranthus amboinicus. The ulcer was proven to be allergic contact dermatitis to P. amboinicus by a patch test. The ulcer healed after discontinuation of P. amboinicus. To the best of our knowledge, this is the first reported case of allergic contact dermatitis to P. amboinicus masquerading as chronic leg ulcer. PMID:16364130

  15. Potential Use of Plectranthus amboinicus in the Treatment of Rheumatoid Arthritis

    OpenAIRE

    Jia-Ming Chang; Chun-Ming Cheng; Le-Mei Hung; Yuh-Shan Chung; Rey-Yuh Wu

    2010-01-01

    Plectranthus amboinicus (P. amboinicus) is a folk herb that is used to treat inflammatory diseases or swelling symptoms in Taiwan. We investigated therapeutic efficacy of P. amboinicus in treating Rheumatoid Arthritis (RA) using collagen-induced arthritis animal model. Arthritis was induced in Lewis rats by immunization with bovine type II collagen. Serum anti-collagen IgG, IgM and C-reactive protein (CRP) were analyzed. To understand the inflammation condition of treated animals, production ...

  16. Rcdisterona de Pfaffia tuberosa (Spreng. Hicken Ecdysterone from Pfaffia tuberosa (Spreng. Hicken

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    Nobushigue Nishimoto

    1986-12-01

    Full Text Available As raízes secas da espécie brasileira Pfaffia tuberosa (Spreng. Hicken foram submetidas a extração com metanol. Deste extrato metanólico foram isolados a ecdisterona e o ácido oleanólico, através de processos de separação por HPLC e cromatografia em coluna. Tanto a ecdisterona como o ácido oleanólico foram identificados comparativamenve com os respec­tivos padrões. Análise quantitativa por HPLC mostrou 0,23% de ecdisterona. Este teor foi corsiderado baixo em relação a outra espécie, também brasileira, a Pfaffia iresinoides.In this paper, we wish to report the isolation and identification of ecdysterone from Pfaffia tuberosa (Spreng. Hicken. The roots of Pfaffia tuberosa (Spreng. Hicken were colected in the Mato Grosso State - Brazil. The dried and sliced roots (197g were extracted with hot MeOH. A suspension of the resulting MeOH extract in the water was treated with n-BuOH. The n-BuOH layer was concentrated in vacuo to give a brown viscous oil (5g, wich was chromatographed on silicagel (65g using CHCl3 - MeOH - H2O (80:20:10 lower phase, and 15ml fractions were collected. Fractions 11-13 were concentrated and the residual solid was recrystallized from EtOH to afford oleanolic acid (65mg. The crystalline residue obtained from fractions 44-56 was recrystallized from EtOAC-MeOH to give ecdysterone (87mg. Each compound was identified by direct comparison with an authentic sample. Further HPLC quantitative analysis showed lower content of ecdysterone (0,23% compared with that of Pfaffia iresinoides Spreng.

  17. Antihyperglycemic and antihyperlipidemic activity of plectranthus amboinicus on normal and alloxan-induced diabetic rats.

    Science.gov (United States)

    Viswanathaswamy, A H M; Koti, B C; Gore, Aparna; Thippeswamy, A H M; Kulkarni, R V

    2011-03-01

    The present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of Plectranthus amboinicus in normal and alloxan-induced diabetic rats. Diabetes was induced in Wistar rats by single intraperitoneal administration of alloxan monohydrate (150 mg/kg). Normal as well as diabetic rats were divided into groups (n=6) receiving different treatments. Graded doses (200 mg/kg and 400 mg/kg) of ethanol extract of Plectranthus amboinicus were studied in both normal and alloxan-induced diabetic rats for a period of 15 days. Glibenclamide (600 μg/kg) was used as a reference drug. Oral administration with graded doses of ethanol extract of Plectranthus amboinicus exhibited hypoglycemic effect in normal rats and significantly reduced the peak glucose levels after 120 min of glucose loading. In alloxan-induced diabetic rats, the daily oral treatment with ethanol extract of Plectranthus amboinicus showed a significant reduction in blood glucose. Besides, administration of ethanol extract of Plectranthus amboinicus for 15 days significantly decreased serum contents of total cholesterol, triglycerides whereas HDL-cholesterol, total proteins and calcium were effectively increased. Furthermore, effect of ethanol extract of Plectranthus amboinicus showed profound elevation of serum amylase and reduction of serum lipase. Histology examination showed ethanol extract of Plectranthus amboinicus exhibited almost normalization of damaged pancreatic architecture in rats with diabetes mellitus. Studies clearly demonstrated that ethanol extract of Plectranthus amboinicus leaves possesses hypoglycemic and antihyperlipidemic effects mediated through the restoration of the functions of pancreatic tissues and insulinotropic effect. PMID:22303055

  18. EVALUATION OF PHYTOCHEMICAL AND ANTIBACTERIAL ACTIVITY OF PLECTRANTHUS AMBOINICUS

    Directory of Open Access Journals (Sweden)

    Arunkumar Sathasivam

    2011-02-01

    Full Text Available Plectranthus amboinicus is a commonly available medicinal herb in India. The phytochemical active compounds qualitatively analyzed such as alkaloids, Terpenoids, Cardiac glycosides, saponin, tannins and flavonoids present in Plectranthus amboinicus. The aqueous, acetone and methanol crude plant extracts were prepared and tested against gram positive and gram negative bacteria. The highest antibacterial activities were observed in methanol extract (11± 0.19, 16± 0.45, 15±0.57, 17±0.23, 16± 0.48, 10±0.38 and 16±0.66 mm in diameter compared than acetone extract (6.0 ± 0.18, 6.0 ± 0.18, 6.0 ± 0.18, 6.0 ± 0.18 mm in diameter. There is low antibacterial activity in aqueous extract.

  19. Antihyperglycemic and Antihyperlipidemic Activity of Plectranthus Amboinicus on Normal and Alloxan-Induced Diabetic Rats

    OpenAIRE

    A. H. M. Viswanathaswamy; Koti, B. C.; Aparna Gore; Thippeswamy, A.H.M.; Kulkarni, R. V.

    2011-01-01

    The present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of Plectranthus amboinicus in normal and alloxan-induced diabetic rats. Diabetes was induced in Wistar rats by single intraperitoneal administration of alloxan monohydrate (150 mg/kg). Normal as well as diabetic rats were divided into groups (n=6) receiving different treatments. Graded doses (200 mg/kg and 400 mg/kg) of ethanol extract of Plectranthus amboinicus were studied...

  20. Antineoplasic Effect of Aqueous Extract of Plectranthus Amboinicus in Ehrlich Ascites Carcinoma

    OpenAIRE

    Brandao, Eduardo M.; Brandão, Paulo H. D. M.; Ivone A. Souza; Paiva, Gerson S.; de C. Carvalho, Marcos; Lacerda, Claudio M.

    2013-01-01

    There are 46,000 new cases of peritoneal carcinomatosis per year in the USA and 17,700 in Brazil. New media, including plant derivatives, are being tested in its treatment. Plectranthus amboinicus is a medicinal plant widely used in Brazil, especially in the northeast region, for the treatment of various diseases, including cancer. This present study evaluates the intraperitoneal use of aqueous extracts of Plectranthus amboinicus (AEPa) at a dose of 200 mg / kg for the treatment of the asciti...

  1. Alcoholic leaf extract of Plectranthus amboinicus regulates carbohydrate metabolism in alloxan-induced diabetic rats

    OpenAIRE

    Koti, B. C.; Aparna Gore; Thippeswamy, A.H.M.; A. H. M. Viswanatha Swamy; Rucha Kulkarni

    2011-01-01

    Objective: The present investigation was undertaken to explore the possible mechanisms of Plectranthus amboinicus leaf extract in alloxan-induced diabetic rats. Materials and Methods: Control and alloxan-induced diabetic albino rats received different treatments; orally control (vehicle), 200 mg/kg and 400 mg/kg of ethanol extract of Plectranthus amboinicus (PAEE) and 600 μg/kg of glibenclamide (standard) for 15 days. At the end of the experiment, the animals were sacrificed and enzyme ac...

  2. Antihyperglycemic and antihyperlipidemic activity of Plectranthus amboinicus on normal and alloxan-induced diabetic rats

    Directory of Open Access Journals (Sweden)

    A. H. M. Viswanathaswamy

    2011-01-01

    Full Text Available The present study was undertaken to investigate the antihyperglycemic and antihyperlipidemic effects of ethanol extract of Plectranthus amboinicus in normal and alloxan-induced diabetic rats. Diabetes was induced in Wistar rats by single intraperitoneal administration of alloxan monohydrate (150 mg/kg. Normal as well as diabetic rats were divided into groups (n=6 receiving different treatments. Graded doses (200 mg/kg and 400 mg/kg of ethanol extract of Plectranthus amboinicus were studied in both normal and alloxan-induced diabetic rats for a period of 15 days. Glibenclamide (600 μg/kg was used as a reference drug. Oral administration with graded doses of ethanol extract of Plectranthus amboinicus exhibited hypoglycemic effect in normal rats and significantly reduced the peak glucose levels after 120 min of glucose loading. In alloxan-induced diabetic rats, the daily oral treatment with ethanol extract of Plectranthus amboinicus showed a significant reduction in blood glucose. Besides, administration of ethanol extract of Plectranthus amboinicus for 15 days significantly decreased serum contents of total cholesterol, triglycerides whereas HDL-cholesterol, total proteins and calcium were effectively increased. Furthermore, effect of ethanol extract of Plectranthus amboinicus showed profound elevation of serum amylase and reduction of serum lipase. Histology examination showed ethanol extract of Plectranthus amboinicus exhibited almost normalization of damaged pancreatic architecture in rats with diabetes mellitus. Studies clearly demonstrated that ethanol extract of Plectranthus amboinicus leaves possesses hypoglycemic and antihyperlipidemic effects mediated through the restoration of the functions of pancreatic tissues and insulinotropic effect.

  3. Potential Use of Plectranthus amboinicus in the Treatment of Rheumatoid Arthritis.

    Science.gov (United States)

    Chang, Jia-Ming; Cheng, Chun-Ming; Hung, Le-Mei; Chung, Yuh-Shan; Wu, Rey-Yuh

    2010-03-01

    Plectranthus amboinicus (P. amboinicus) is a folk herb that is used to treat inflammatory diseases or swelling symptoms in Taiwan. We investigated therapeutic efficacy of P. amboinicus in treating Rheumatoid Arthritis (RA) using collagen-induced arthritis animal model. Arthritis was induced in Lewis rats by immunization with bovine type II collagen. Serum anti-collagen IgG, IgM and C-reactive protein (CRP) were analyzed. To understand the inflammation condition of treated animals, production of TNF-α, IL-6 and IL-1β from peritoneal exudates cells (PEC) were also analyzed. P. amboinicus significantly inhibited the footpad swelling and arthritic symptoms in collagen-induced arthritic rats, while the serum anti-collagen IgM and CRP levels were consistently decreased. The production of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were also decreased in the high dosage of P. amboinicus group. Here, we demonstrate the potential anti-arthritic effect of P. amboinicus for treating RA, which might confer its anti-rheumatic activity. This differs the pharmacological action mode of indomethacin. PMID:18955284

  4. Potential Use of Plectranthus amboinicus in the Treatment of Rheumatoid Arthritis

    Directory of Open Access Journals (Sweden)

    Jia-Ming Chang

    2010-01-01

    Full Text Available Plectranthus amboinicus (P. amboinicus is a folk herb that is used to treat inflammatory diseases or swelling symptoms in Taiwan. We investigated therapeutic efficacy of P. amboinicus in treating Rheumatoid Arthritis (RA using collagen-induced arthritis animal model. Arthritis was induced in Lewis rats by immunization with bovine type II collagen. Serum anti-collagen IgG, IgM and C-reactive protein (CRP were analyzed. To understand the inflammation condition of treated animals, production of TNF-α, IL-6 and IL-1β from peritoneal exudates cells (PEC were also analyzed. P. amboinicus significantly inhibited the footpad swelling and arthritic symptoms in collagen-induced arthritic rats, while the serum anti-collagen IgM and CRP levels were consistently decreased. The production of pro-inflammatory cytokines TNF-α, IL-6 and IL-1β were also decreased in the high dosage of P. amboinicus group. Here, we demonstrate the potential anti-arthritic effect of P. amboinicus for treating RA, which might confer its anti-rheumatic activity. This differs the pharmacological action mode of indomethacin.

  5. Antimalarial activity of Malaysian Plectranthus amboinicus against Plasmodium berghei

    Directory of Open Access Journals (Sweden)

    Norazsida Ramli

    2014-01-01

    Full Text Available Context: Malaria is a mosquito-borne disease caused by parasitic protozoa from the genus of Plasmodium. The protozoans have developed resistance against many of current drugs. It is urgent to find an alternative source of new antimalarial agent. In the effort to discover new antimalarial agents, this research has been conducted on Plectranthus amboinicus. Aims: This study was conducted to evaluate the toxicity and antiplasmodial properties of P. amboinicus. Materials and Methods: Acute oral toxicity dose at 5000 mg/kg was conducted to evaluate the safety of this extract. Twenty mice were divided into control and experimental group. All the mice were observed for signs of toxicity, mortality, weight changes and histopathological changes. Antimalarial activity of different extract doses of 50, 200, 400 and 1000 mg/kg were tested in vivo against Plasmodium berghei infections in mice (five mice for each group during early, established and residual infections. Results: The acute oral toxicity test revealed that no mortality or evidence of adverse effects was seen in the treated mice. The extract significantly reduced the parasitemia by the 50 (P = 0.000, 200 (P = 0.000 and 400 mg/kg doses (P = 0.000 in the in vivo prophylactic assay. The percentage chemo-suppression was calculated as 83.33% for 50 mg/kg dose, 75.62% for 200 mg/kg dose and 90.74% for 400 mg/kg dose. Body weight of all treated groups; T1, T2, T3 and T4 also showed enhancement after 7 days posttreatment. Statistically no reduction of parasitemia calculated for curative and suppressive test. Conclusion: Thus, this extract may give a promising agent to be used as a prophylactic agent of P. berghei infection.

  6. Effects of shading on Vallisneria natans (Lour.) H. Hara growth

    DEFF Research Database (Denmark)

    Fox, Anthony David; Meng, F; Shen, X;

    2013-01-01

    Effects of surface shading were measured on above- and below-ground biomass and fruit production of Vallisneria natans (Lour.) H. Hara plants grown from seed in replicated microcosm experiments, based on a control (no shading) and four treatments (25%, 50%, 75% and 90% shading). Above- and below...

  7. Antineoplasic effect of aqueous extract of plectranthus amboinicus in ehrlich ascites carcinoma.

    Science.gov (United States)

    Brandao, Eduardo M; Brandão, Paulo H D M; Souza, Ivone A; Paiva, Gerson S; de C Carvalho, Marcos; Lacerda, Claudio M

    2013-01-01

    There are 46,000 new cases of peritoneal carcinomatosis per year in the USA and 17,700 in Brazil. New media, including plant derivatives, are being tested in its treatment. Plectranthus amboinicus is a medicinal plant widely used in Brazil, especially in the northeast region, for the treatment of various diseases, including cancer. This present study evaluates the intraperitoneal use of aqueous extracts of Plectranthus amboinicus (AEPa) at a dose of 200 mg / kg for the treatment of the ascitic form of Ehrlich carcinoma. It is concluded also the AEPa produced antineoplastic effect in ascitic form of Ehrlich carcinoma. PMID:23983822

  8. Chemical constituents of Plectranthus amboinicus and the synthetic analogs possessing anti-inflammatory activity.

    Science.gov (United States)

    Chen, Yuan-Siao; Yu, Hui-Ming; Shie, Jiun-Jie; Cheng, Ting-Jen Rachel; Wu, Chung-Yi; Fang, Jim-Min; Wong, Chi-Huey

    2014-03-01

    This study demonstrates that compounds 1-4 from an extract of Plectranthus amboinicus inhibit the binding of AP-1 to its consensus DNA sequence. Thymoquinone (5) was further identified as a nonpolar ingredient from the hexane extract of P. amboinicus to suppress the expression of lipopolysaccharide-induced tumor necrosis factor-alpha (TNF-α). We then synthesized 2-alkylidenyl-4-cyclopentene-1,3-diones as the designed biomimetics of thymoquinone, and found that compounds 8a, 8b and 8d were more potent TNF-α inhibitors. PMID:24491635

  9. Effects of shading on Vallisneria natans (Lour.) H. Hara growth

    OpenAIRE

    Fox A.D.; Meng F; Shen X; Yang X; Yang W; Cao L

    2013-01-01

    Effects of surface shading were measured on above- and below-ground biomass and fruit production of Vallisneria natans (Lour.) H. Hara plants grown from seed in replicated microcosm experiments, based on a control (no shading) and four treatments (25%, 50%, 75% and 90% shading). Above- and below-ground biomass was significantly reduced at treatments above 50% shading and first pistillate and staminate florescence dates were sign...

  10. Alkenes with antioxidative activities from Murraya koenigii (L.) Spreng.

    Science.gov (United States)

    Ma, Qin-Ge; Xu, Kun; Sang, Zhi-Pei; Wei, Rong-Rui; Liu, Wen-Min; Su, Ya-Lun; Yang, Jian-Bo; Wang, Ai-Guo; Ji, Teng-Fei; Li, Lu-Jun

    2016-02-01

    Four new alkenes (1-4), and six known alkenes (5-12) were isolated from Murraya koenigii (L.) Spreng. Their structures were elucidated on the basis of spectroscopic analyses and references. Compounds (1-12) were evaluated for antioxidative activities. Among them, compounds 1, 2, 4, and 7 exhibited significant antioxidative activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay with IC50=21.4-49.5 μM. The known compounds (5-12) were isolated from this plant for the first time.

  11. Evaluación de la toxicidad aguda de extractos de plantas medicinales por un método alternativo (Evaluation of acute toxicity of extracts of medicinal plants by an alternative testing)

    OpenAIRE

    Deodelsy Bermúdez Toledo:; |Emilio Monteagudo Jimenez; Maria Boffill Cárdenas; Luis E. Díaz Costa; Alexander Roca Simeón; Emoe Betancourt Morgado; Silveira Prado, Enrique A

    2007-01-01

    Se evaluó la toxicidad a dosis única de seis extractos de plantas medicinales: Psidium guajava L. (guayaba), Eucalyptus citriodoraHooker (eucalipto de limón), Cymbopogon citratus (DC) Stapf (caña santa, hierba de limón), Hibiscus elatus Sw. (majagua), Justicia pectoralis Jacq. (tilo), Plectranthus amboinicus (Lour.) Spreng (orégano francés) mediante un método alternativo e internacionalmente validado y aceptado (procedimiento de dosis fijas) en ratasSprague Dawley. El grupo tratado recibió po...

  12. Preliminary phytochemical screening of the pericarp of Crataeva magna (Lour.) DC. - a medicinal tree

    Institute of Scientific and Technical Information of China (English)

    Solomon Kiruba; Mony Mahesh; Zachariah Miller Paul; Solomon Jeeva

    2011-01-01

    Objective: To examine the phytochemicals present in the pericarp of Crataeva magna (C. magna) (Lour.) DC. which is used as a traditional medicine by the inhabitants of Kanyakumari district. Methods: Phytochemical screening of the pericarp was done to determine the secondary metabolites in various solvents studied. Results: The phytochemical screening on the pericarp of C. magna (Lour.) DC. proved the presence of phytochemicals such as phenols, saponins and tannins. Conclusions: The findings of the present study recommended that the pericarp of C. magna (Lour.) DC. have potential antimicrobial compounds that may be of use for developing plant based drugs for various ailments.

  13. Efek Anti Radang dan Toksisitas Akut Ekstrak Daun Jintan (Plectranthus amboinicus) pada Tikus yang Diinduksi Arthritis

    OpenAIRE

    Lailatul Muniroh; Santi Martini; Triska Susila Nindya; Rondius Solfaine

    2013-01-01

    Abstrak Tanaman Jintan (Plectranthus amboinicus) dikenal sebagai tanaman bangun-bangun, dikenal sebagai salah satu tanaman berkhasiat obat bagi masyarakat Indonesia. Penelitian ini bertujuan menganalisis aktivitas ekstrak daun jintan dan mengetahui efek toksisitas akut pada tikus putih yang diinduksi arthritis. Ekstrak dari daun jintan segar disarikan dengan metode maserasi ethanol 96%, dan diencerkan dengan larutan CMC-Na. Tikus putih Wistar jantan dan betina, umur 2-3 bulan ...

  14. Chemical Composition and Nutraceutical Potential of Indian Borage (Plectranthus amboinicus) Stem Extract

    OpenAIRE

    Praveena Bhatt; Gilbert Stanley Joseph; Pradeep Singh Negi; Mandyam Chakravarthy Varadaraj

    2013-01-01

    The stem of Indian borage (Plectranthus amboinicus) was found to be an antioxidant rich fraction as evaluated by in vitro models such as DPPH free radical scavenging, reducing power assay, superoxide anion radical scavenging, and total antioxidant capacity. The extract also exhibited antiplatelet aggregation ability, antibacterial activity, and antiproliferative effect against cancer cell lines: Caco-2, HCT-15, and MCF-7. Phytochemical evaluation of the extract revealed the occurrence of tot...

  15. EFFECT OF ETHANOLIC EXTRACT OF PLECTRANTHUS AMBOINICUS LEAF ON HEALING OF BURN WOUND IN WISTAR RATS

    OpenAIRE

    Smita Shenoy; Sukesh,; Vinod MS; Shruthi; Mohan Amberkar; Arul Amuthan

    2012-01-01

    Objectives: To study the effect of ethanolic extract of the leaf of Plectranthus amboinicus on the healing of burn wounds in wistar rats and compare it with silver sulfadiazine treated group. Materials and Methods: Five groups of rats were used in the study. Partial thickness burn wounds were made on each rat under ketamine anesthesia.The wounds in the five groups of rats were treated topically with petroleum base, silver sulfadiazine,1%, 2% and 3% ointment of ethanolic extract of Plectran...

  16. EFFECT OF ETHANOLIC EXTRACT OF PLECTRANTHUS AMBOINICUS LEAF ON HEALING OF BURN WOUND IN WISTAR RATS

    Directory of Open Access Journals (Sweden)

    Smita Shenoy

    2012-08-01

    Full Text Available Objectives: To study the effect of ethanolic extract of the leaf of Plectranthus amboinicus on the healing of burn wounds in wistar rats and compare it with silver sulfadiazine treated group. Materials and Methods: Five groups of rats were used in the study. Partial thickness burn wounds were made on each rat under ketamine anesthesia.The wounds in the five groups of rats were treated topically with petroleum base, silver sulfadiazine,1%, 2% and 3% ointment of ethanolic extract of Plectranthus amboinicus, respectively, once daily for 21 days or till complete healing whichever was earlier. The wound contraction rate and period of epithelization were monitored. Results: The rate of wound contraction was significantly more in Plectranthusamboinicustreated groups in comparison to the control. The mean period of epithelization was significantly decreased in Plectranthus amboinicus treated group when compared to control (P < 0.01 and silver sulfadiazine (P =0.02 treated group. Conclusion: The ethanolic extract of Plectranthusamboinicus promoted healing of burn wound in wistar rats.

  17. Cytotoxic Constituents from the Stems of Clausena lansium (Lour. Skeels

    Directory of Open Access Journals (Sweden)

    Shu Shan Du

    2013-09-01

    Full Text Available Six compounds were isolated from the stems of Clausena lansium (Lour. Skeels by repeated sillica gel column chromatography. Their chemical structures were elucidated on the basic of physicochemical and spectroscopic data. Among them, 8-geranyloxypsolaren (3 and 2-methoxy-1-(3-methyl-buten-1-yl-9H-carbazole-3-carbaldehyde (6 were isolated for the first time from this plant. These compounds were screened for cytotoxicity in human cervical cancer (Hela, leukemia (K562, lung cancer (A549, non-small lung carcinoma (H1299 and liver cancer (SMMC-7721. Within the series of cytotoxic tests, compounds 4–6 displayed potent cytotoxic activity against H1299 and SMMC-7721, with the IC50 values of 6.19 to 26.84 μg/mL.

  18. In vitro antioxidant studies of Dioscorea esculenta (Lour). Burkill

    Institute of Scientific and Technical Information of China (English)

    Manickam Murugan; Veerabahu Ramasamy Mohan

    2012-01-01

    Objective: To evaluate the total phenolic, flavonoid contents and in vitro antioxidant activity of methanol extract of Dioscorea esculenta (Lour). Burkill. Methods: Total phenolic content was estimated using the Folin Ciocalteu method. The flavonoid content was determined using aluminium chloride. In vitro antioxidant activities and reducing power capacity were determined using standard methods. Results: Total phenolic content in methanol extract of Dioscorea esculenta was found to be 0.79g/100g and flavonoids content was found to be 0.26 g/100g. The extract was screened for its potential antioxidant activities using tests such as DPPH radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power activity. Conclusions: The present studies confirm the methanol extracts have potential in vitro antioxidant activity. The phytochemical phenols and flavonoids could be the reason for its antioxidant activity.

  19. PATHORCHUR (COLEUS AROMATICUS: A REVIEW OF THE MEDICINAL EVIDENCE FOR ITS PHYTOCHEMISTRY AND PHARMACOLOGY PROPERTIES

    Directory of Open Access Journals (Sweden)

    Om Prakash Rout

    2012-12-01

    Full Text Available Coleus aromaticus Benth., (Fam. Lamiaceae, syn. Coleus amboinicus Lour. Spreng or Plectranthus ambonicus Lour, is commonly known as Indian/ country borage and ‘Pathorchur’ in Hindi and Bengali. It is recorded in the Indian system of medicine as one of the sources of Pashanabheda. It is large succulent aromatic perennial herb, shrubby below, hispidly villous or tomentose. It is found throughout India, Ceylon and Moluccas. The leaves of the plant are bitter, acrid and were being widely used traditionally for various purposes. The plant has been worked out very well and isolated several chemical constituents and had shown various biological properties. This review is an effort to compile all the information reported on its macroscopic, microscopic features, nutritional content, phytochemistry, pharmacology and therapeutic uses.

  20. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus leaf extracts.

    Science.gov (United States)

    Ethanolic extracts of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus were tested for toxicity to 3rd instar Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti. Median lethal concentrations (LC50) were, respectively, 0.44%, 0.51%, 0.59% and 0.68%. Cynodon dactylon...

  1. Effects of shading on Vallisneria natans (Lour. H. Hara growth

    Directory of Open Access Journals (Sweden)

    Fox A.D.

    2013-08-01

    Full Text Available Effects of surface shading were measured on above- and below-ground biomass and fruit production of Vallisneria natans (Lour. H. Hara plants grown from seed in replicated microcosm experiments, based on a control (no shading and four treatments (25%, 50%, 75% and 90% shading. Above- and below-ground biomass was significantly reduced at treatments above 50% shading and first pistillate and staminate florescence dates were significantly delayed above 75% and 50% shading, respectively. Ratios of mature to unripe fruits produced (both in number or dry weight did not differ between shading treatments, but dry weight fruit production was significantly reduced at 90% shading. We conclude that above 50% surface shading, V. natans plants suffer reductions in accumulated biomass and investment in sexual reproduction. We contend that recent expansions in the extent of the native floating water chestnut Trapa spp. at seasonally inundated wetlands in the Yangtze River floodplain could, by shading, have contributed to the reduction in annual biomass and seed production of V. natans, contributing to declines in distribution and abundance.

  2. Phytotoxicity and Cytotoxicity of Essential Oil from Leaves of Plectranthus amboinicus, Carvacrol, and Thymol in Plant Bioassays.

    Science.gov (United States)

    Pinheiro, Patrícia Fontes; Costa, Adilson Vidal; Alves, Thammyres de Assis; Galter, Iasmini Nicoli; Pinheiro, Carlos Alexandre; Pereira, Alexandre Fontes; Oliveira, Carlos Magno Ramos; Fontes, Milene Miranda Praça

    2015-10-21

    The essential oil of Plectranthus amboinicus and its chemotypes, carvacrol and thymol, were evaluated on the germination and root and aerial growth of Lactuca sativa and Sorghum bicolor and in acting on the cell cycle of meristematic root cells of L. sativa. The main component found in the oil by analysis in gas chromatography-mass spectrometry and gas chromatography flame ionization detection was carvacrol (88.61% in area). At a concentration of 0.120% (w v(-1)), the oil and its chemotypes retarded or inhibited the germination and decreased root and aerial growth in monocot and dicot species used in the bioassays. In addition, all substances caused changes in the cell cycle of the meristematic cells of L. sativa, with chromosomal alterations occurring from the 0.015% (w v(-1)) concentration. The essential oil of P. amboinicus, carvacrol, and thymol have potential for use as bioherbicides. PMID:26416575

  3. Penggunaan Ekstrak Buah Barberry (Berberis Nepalensis (DC.) Spreng.) Sebagai Pewarna Dalam Sediaan Lipstik

    OpenAIRE

    Sinurat, Novalina

    2013-01-01

    Barberry fruits (Berberis nepalensis (DC.) Spreng.) is one of millions biological resources which has a potention to be used as a natural colorant, because it has an attractive color. Barberry reddish purple color caused by anthocyanin pigment which can be used as a natural colorant. Natural colorant can be used to avoid a synthetic colorant that possible dangerous our health. The aim of this study was to make lipstick formula using natural colorant from barberry fruits extract. Barberr...

  4. Pharmacological evaluation of Musa seminifera Lour.fruit

    Institute of Scientific and Technical Information of China (English)

    Sanjib Saha; Faroque Hossain; Md.Anisuzzman; Md.Khirul Islam

    2013-01-01

    OBJECTIVE:To study potential antioxidant,analgesic,antidiarrheal,and antibacterial activities of the ethanol extract of Musa seminifera Lour.fruit in different established in vivo and in vitro experimental models.METHODS:In vitro antioxidant activity was studied in 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay.Phenolic content was determined using Folin-Ciocalteu's reagent.Reducing ability was evaluated by ferric reducing power assay.Peripherally and centrally acting analgesic activity was studied in three different in vivo models,namely,acetic acid-induced writhing,hot-plate test,and tail-flick test in Swiss albino mice.In vivo antidiarrheal activity was evaluated in castor oil-and magnesium sulfate-induced diarrhea in mice.Gastrointestinal motility test was also carried out in mice.All studies in mice were undertaken at the doses of 250 and 500 mg/kg body weight.Antibacterial activity was assessed by disk diffusion assay against some Gram-positive and Gram-negative bacterial strains.Acute toxicity test was conducted to assess the safe doses of the extract.RESULTS:The extract showed 50% inhibitory concentration value of 12.65 μg/mL in DPPH radicalscavenging assay.Phenolic content was found to be 589.83 mg gallic acid equivalent per 100 g of dried fruits extract.Reducing power was in a concentration-dependent manner,and strongly comparable with the standard ascorbic acid.The extract demonstrated significant inhibition of writhing in acetic acid-induced writhing test at both dose levels (P<0.01).The extract also raised pain threshold in both hot-plate and tail-flick test in a dose-dependent manner,and the results were statistically significant (P<0.01).The extract significantly (P<0.01) increased latent period,and decreased defecation in both castor oil-and magnesium sulfate-induced diarrhea.The extract also decreased gastrointestinal motility in mice.In disk diffusion assay,the extract showed potential antibacterial activity against all the

  5. Uji In Vitro Aktivitas Antelmintik Ekstrak Etanol Daun Pugun Tanoh [Curanga fel-terrae (Lour.) Merr.

    OpenAIRE

    Ginting, Grace Anastasia br

    2016-01-01

    Background: Ethanolic extract of Curanga fel-terrae (Lour.) Merr. leaves obtained by maceration has anthelmintic activity against Pheretima posthuma. However the anthelmintic activity is affected by extraction method. Soxhletation can extract more chemical compounds of plant than maceration. Objective: Aim of this study was to evaluate the in vitro anthelmintic activity of leaf ethanolic extract of C. fel-terrae obtained by soxhletation against P. posthuma. Methods: Dried material of C....

  6. Efek Anti Radang dan Toksisitas Akut Ekstrak Daun Jintan (Plectranthus amboinicus pada Tikus yang Diinduksi Arthritis

    Directory of Open Access Journals (Sweden)

    Lailatul Muniroh

    2013-06-01

    Full Text Available Abstrak Tanaman Jintan (Plectranthus amboinicus dikenal sebagai tanaman bangun-bangun, dikenal sebagai salah satu tanaman berkhasiat obat bagi masyarakat Indonesia. Penelitian ini bertujuan menganalisis aktivitas ekstrak daun jintan dan mengetahui efek toksisitas akut pada tikus putih yang diinduksi arthritis. Ekstrak dari daun jintan segar disarikan dengan metode maserasi ethanol 96%, dan diencerkan dengan larutan CMC-Na. Tikus putih Wistar jantan dan betina, umur 2-3 bulan dibagi 5 kelompok: Kontrol, induksi arthritis (P1, induksi artritis dan ekstrak daun jintan dosis 19 g/kgBB (P2, induksi artritis dan ekstrak daun jintan dosis 38 g/kgBB (P3 dan kelompok obat allopurinol 2,5 mg/kgBB (P4. Seluruh kelompok tikus diinduksi arthritis menggunakan uric acid2% dan oxonic acid1,5% per oralselama 15 hari berturut-turut. Setelah terbentuk lesi arthritis, diberikan ekstrak daun jintan secara intra peritoneal selama 7 hari. Sampel serum darah diambil sebelum dan sesudah perlakuan untuk mengukur konsentrasi monosodium urea (MSU. Uji toksisitas akut menggunakan 4 kelompok tikus putih Wistar jantan dan betina yang diberi ekstrak daun jintan mulai dosis 1900 mg/kg BB, 3800 mg/kgBB dan 5000 mg/kgBB.Hasil penelitian menunjukkan ekstrak daun jintan secara kualitatif mempunyai kandungan senyawa Flavonoid, Saponin, Polifenol, Terpen dan Antrakuinon. Uji aktivitas ekstrak daun jintan memperlihatkan penurunan konsentrasi MSU (p 0,05 sebelum dan sesudah perlakuan. Uji toksisitas akut ekstrak daun jintan tidak menimbulkan kematian 50% (LD50 dan tidak menimbulkan gejala toksik, gangguan syarafi dan penurunan aktivitas pada semua kelompok perlakuan sehingga ekstrak daun jintan dapat digolongkan sebagai bahan yang “praktis tidak toksik”. Abstract Anti Inflammation Effects and Acute Toxicity of Jintan Leaves (Plectranthus amboinicus Extract on Arthritis Induced Rats.Jintan plant (Plectranthus amboinicus is known as bangun-bangun plant and known as one of medicinal

  7. Chemical Fingerprinting of the Fragrant Volatiles of Nineteen Indian Cultivars of Cymbopogon Spreng. (Poaceae)

    OpenAIRE

    Rajendra C. Padalia; Verma, Ram S.; Chanotiya, Chandan S; Anju Yadav

    2011-01-01

    The essential oil compositions of total nineteen cultivars of Cymbopogon Spreng. (Poaceae) species viz. C. martinii (Roxb.) Wats. var. motia Burk., C. flexuosus Nees ex Steud, C. winterinus Jowitt., C. pendulus Nees ex Steud. and a hybrid of C. khasianus (Hack) Stapf. ex Bor and C. pendulus Nees ex Steud. were examined and compared using capillary GC and GC-MS . The analysis led to the identification of 48 constituents forming 90.1% to 99.7% of their total oil compositions with monoterpenoids...

  8. Anatomia do xilema secundário de Mimosa incana (Spreng. Benth.

    Directory of Open Access Journals (Sweden)

    José Newton Cardoso Marchiori

    2009-09-01

    Full Text Available Normal 0 21 false false false MicrosoftInternetExplorer4 São descritos os caracteres anatômicos do xilema secundário de Mimosa incana (Spreng. Benth. e fornecidas 29 medições de sua estrutura. A descrição é ilustrada com fotomicrografias. A estrutura anatômica indica um alto grau de especialização filogenética e reúne diversos caracteres indicativos da família Leguminosae, sub-família Mimosoideae e série Lepidotae Benth. Normal 0 21 false false false MicrosoftInternetExplorer4

  9. Evaluation of nephroprotective, diuretic, and antioxidant activities of plectranthus amboinicus on acetaminophen-induced nephrotoxic rats.

    Science.gov (United States)

    Palani, S; Raja, S; Naresh, R; Kumar, B Senthil

    2010-05-01

    Plectranthus amboinicus (PA), commonly known as country borage, is a folkoric medicinal plant. Juice from its leaves is commonly used for illnesses including liver and renal conditions in the Asian sub-continent. Acetaminophen (APAP), used as an analgesic, produces liver and kidney necrosis in mammals at high doses. The aim of this study was to investigate the nephroprotective, diuretic, and antioxidant activities of the ethanol extract of PA at two doses of 250 and 500 mg/kg bw on APAP-induced toxicity in rats. This study shows that APAP significantly increases the levels of serum urea (UR), hemoglobin (Hb), total leukocyte count, creatinine, raised body weight, and reduced levels of neutrophils, granulocytes, uric acid, and platelet concentration. Ethanol extract of PA rescued these phenotypes by increasing anti-oxidative responses as assessed by biochemistry and histopathology. In addition, the ethanol extract of PA at two doses showed a significant diuretic activity by increased levels of total urine output and urinary elerolytes such as sodium and potassium. In conclusion, these data suggest that the ethanol extract of PA possess nephroprotective and antioxidant effects against APAP-induced nephrotoxicity and strong diuretics effect in rats. PMID:20367443

  10. Biosynthesis of silver nanoparticles using Plectranthus amboinicus leaf extract and its antimicrobial activity

    Science.gov (United States)

    Ajitha, B.; Ashok Kumar Reddy, Y.; Sreedhara Reddy, P.

    2014-07-01

    This study reports the simple green synthesis method for the preparation of silver nanoparticles (Ag NPs) using Plectranthus amboinicus leaf extract. The pathway of nanoparticles formation is by means of reduction of AgNO3 by leaf extract, which acts as both reducing and capping agents. Synthesized Ag NPs were subjected to different characterizations for studying the structural, chemical, morphological, optical and antimicrobial properties. The bright circular fringes in SAED pattern and diffraction peaks in XRD profile reveals high crystalline nature of biosynthesized Ag NPs. Morphological studies shows the formation of nearly spherical nanoparticles. FTIR spectrum confirms the existence of various functional groups of biomolecules capping the nanoparticles. UV-visible spectrum displays single SPR band at 428 nm indicating the absence of anisotropic particles. The synthesized Ag NPs exhibited better antimicrobial property towards gram negative Escherichia coli and towards tested Penicillium spp. than other tested microorganisms using disc diffusion method. Finally it has proven that the synthesized bio-inspired Ag NPs have potent antimicrobial effect.

  11. Chemical Composition and Nutraceutical Potential of Indian Borage (Plectranthus amboinicus Stem Extract

    Directory of Open Access Journals (Sweden)

    Praveena Bhatt

    2013-01-01

    Full Text Available The stem of Indian borage (Plectranthus amboinicus was found to be an antioxidant rich fraction as evaluated by in vitro models such as DPPH free radical scavenging, reducing power assay, superoxide anion radical scavenging, and total antioxidant capacity. The extract also exhibited antiplatelet aggregation ability, antibacterial activity, and antiproliferative effect against cancer cell lines: Caco-2, HCT-15, and MCF-7. Phytochemical evaluation of the extract revealed the occurrence of total phenolics (49.91 mg GAE/g extract, total flavonoids (26.6 mg RE/g extract, and condensed tannins (0.7 mg TAE/g extract. Among the major phenolics, rosmarinic acid (6.160 mg/g extract was predominant, followed by caffeic acid (0.770 mg/g extract, rutin (0.324 mg/g extract, gallic acid (0.260 mg/g extract, quercetin (0.15 mg/g extract, and p-coumaric acid (0.104 mg/g extract. The appreciable biological activity and presence of biomolecules in the methanolic extract of stem indicate its potential application as functional food ingredients and nutraceuticals.

  12. Comparação entre os metais presentes em Croton floribundus Spreng. e Baccharis dracunculifolia DC.

    Directory of Open Access Journals (Sweden)

    Patrícia A.Telles Macari

    2002-01-01

    Full Text Available Metals are very important in the vegetal fisiology, if so, it's necessary their quantification. In the soil the plants are acumulatives and detectors of metals. The medicinal plants Croton floribundus Spreng. and Baccharis dracunculifolia DC. were collected in the Floresta Nacional de Ipanema (FLONA - Iperó - SP. The analysis method used the Atomic Absorption Spectrometry.

  13. (210)Po and (210)Pb in medicinal plants in the region of Karnataka, Southern India.

    Science.gov (United States)

    Chandrashekara, K; Somashekarappa, H M

    2016-08-01

    The activity concentrations of naturally occurring radionuclides (210)Po and (210)Pb were estimated in some selected medicinal plants and soil samples of coastal Karnataka in India. The mean activity concentrations of (210)Po and (210)Pb varied in the range of 4.7-42.9 Bq kg(-1) (dry weight) and 36.1-124 Bq kg(-1) (dry weight) in the soil samples, and 3.3-63.7 Bq kg(-1) (dry weight) and 12.0-406 Bq kg(-1) (dry weight), in the medicinal plant samples, respectively. The plants, Ocimum sanctum L. and Plectranthus amboinicus (Lour.) Spreng had significantly higher activity concentrations of (210)Po and (210)Pb than other species sampled. In spite of disequilibrium between them, these two radionuclides were well correlated in both soil and medicinal plants. PMID:27155527

  14. Kuidas maikrahv uued rõivad sai. Leopold von Pezoldi "Maikrahvi sissesõit Tallinna" ja Theodor Albert Sprengeli "Reformatsiooni algus Tallinnas" avalik konserveerimine = How the May King got his new clother. Public conservation of Leopold von Pezol

    Index Scriptorium Estoniae

    Aas, Kristina, 1988-

    2015-01-01

    Ülevaade baltisaksa kunstnike Leopold von Pezoldi ja Theodor Albert Sprengeli maalidest Suurgildi hoones, nende varasematest restaureerimistest, avalikust konserveerimisest ja tehnilistest uuringutest.

  15. Rhizofiltration of lead using an aromatic medicinal plant Plectranthus amboinicus cultured in a hydroponic nutrient film technique (NFT) system.

    Science.gov (United States)

    Ignatius, A; Arunbabu, V; Neethu, J; Ramasamy, E V

    2014-11-01

    Heavy metal contamination of water bodies and groundwater is a major concern of the modern world. Rhizofiltration--the use of plant root system to remove/extract pollutants from wastewater--has proven advantages over conventional methods of treatment. However, commercialization of this in situ remediation technology requires a better understanding of plant-metal interactions especially on the ability of different plant species to accumulate metals at different parts of the plant system which is critical for the successful remediation of contaminated medium. Many aquatic and terrestrial plants have been reported to accumulate heavy metals when grown hydroponically. Therefore, a batch experiment with different concentrations of lead and a nutrient film technique (NFT) experiment with recycling of wastewater were employed in this study in order to investigate the rhizofiltration of lead-containing wastewater using Plectranthus amboinicus, an aromatic medicinal plant. Results show that P. amboinicus is tolerant to a wide range of lead concentrations and nutrient deficiency. The plant accumulates considerable amount of lead, particularly in the roots, and translocation to the stem and leaf was limited, indicating that the use of leaves/above-ground parts of the plant for medicinal purposes is not hindered by its ability to remove lead from the soil or water. The study also suggests that the plant can be considered for the clean-up of lead-contaminated wastewater in combination with safe biomass disposal alternatives. PMID:24994103

  16. Rhizofiltration of lead using an aromatic medicinal plant Plectranthus amboinicus cultured in a hydroponic nutrient film technique (NFT) system.

    Science.gov (United States)

    Ignatius, A; Arunbabu, V; Neethu, J; Ramasamy, E V

    2014-11-01

    Heavy metal contamination of water bodies and groundwater is a major concern of the modern world. Rhizofiltration--the use of plant root system to remove/extract pollutants from wastewater--has proven advantages over conventional methods of treatment. However, commercialization of this in situ remediation technology requires a better understanding of plant-metal interactions especially on the ability of different plant species to accumulate metals at different parts of the plant system which is critical for the successful remediation of contaminated medium. Many aquatic and terrestrial plants have been reported to accumulate heavy metals when grown hydroponically. Therefore, a batch experiment with different concentrations of lead and a nutrient film technique (NFT) experiment with recycling of wastewater were employed in this study in order to investigate the rhizofiltration of lead-containing wastewater using Plectranthus amboinicus, an aromatic medicinal plant. Results show that P. amboinicus is tolerant to a wide range of lead concentrations and nutrient deficiency. The plant accumulates considerable amount of lead, particularly in the roots, and translocation to the stem and leaf was limited, indicating that the use of leaves/above-ground parts of the plant for medicinal purposes is not hindered by its ability to remove lead from the soil or water. The study also suggests that the plant can be considered for the clean-up of lead-contaminated wastewater in combination with safe biomass disposal alternatives.

  17. Streblus asper Lour. (Shakhotaka: A Review of its Chemical, Pharmacological and Ethnomedicinal Properties

    Directory of Open Access Journals (Sweden)

    Subha Rastogi

    2006-01-01

    Full Text Available Streblus asper Lour is a small tree found in tropical countries, such as India, Sri Lanka, Malaysia, the Philippines and Thailand. Various parts of this plant are used in Ayurveda and other folk medicines for the treatment of different ailments such as filariasis, leprosy, toothache, diarrhea, dysentery and cancer. Research carried out using different in vitro and in vivo techniques of biological evaluation support most of these claims. This review presents the botany, chemistry, traditional uses and pharmacology of this medicinal plant.

  18. CONTRIBUTION TO THE WOOD ANATOMY OF TINOSPORA SINENSIS (LOUR) MERRILL IN RELATION WITH T.CORDIFOLIA MIERS

    OpenAIRE

    Bonde, S.D.; Upaehye, A.S.

    1989-01-01

    Morphologically Tincspora sinensis (Lour.) Merrill is differentiated from T. cordifolia Miers. Only by the tomentose leaves. The present anatomical study aids in differentiating pieces of woods of T. sinensis from those of T. cordifolia which is often mixed as adulterant or substitute for T. cordifolia in several Ayurvedic compound preparations.

  19. Foliar fertilization as an alternative to hand thinning fruit in chlorate treated Dimocarpus longan Lour. trees in Hawaii.

    Science.gov (United States)

    Longan, Dimocarpus longan Lour, is a member of the Sapindaceae, a family that also includes lychee, and rambutan. The discovery of potassium chlorate (KClO3) induced flowering solved the problem of alternate bearing and enabled the grower to produce off-season longan. Chlorate treatments commonly i...

  20. Chemical composition and antimicrobial activity of the essential oil from Aeolanthus suaveolens Mart. ex Spreng

    Energy Technology Data Exchange (ETDEWEB)

    Simionatto, Euclesio [Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS (Brazil). Dept. de Hidraulica e Transportes]. E-mail: eusimionatto@yahoo.com.br; Porto, Carla; Stueker, Caroline Z.; Dalcol, Ionara I.; Silva, Ubiratan F. da [Universidade Federal de Santa Maria (UFSM), RS (Brazil). Dept. de Quimica

    2007-07-01

    The essential oils from leaves (sample A) and flowers (sample B) of Aeolanthus suaveolens Mart. ex Spreng were obtained by hydro distillation and analyzed by GC, GC-MS, and chiral phase gas chromatography (CPGC). Six compounds have been identified from the essential oils, representing ca 94.3 and 93% of the oils corresponding to samples A and B, respectively. The major constituents of samples A and B essential oils were respectively, linalool (34.2%/34.9%), (-)-massoialactone (25.9%/17.0%) and (E)-beta-farnesene (25.4%/29.1%). The enantiomeric distribution of the monoterpene linalool was established by analysis on heptakis-(6-O-methyl-2,3-di-O-pentyl)-beta-cyclodextrin capillary column. The antimicrobial activity of the essential oil from leaves and isolated compounds was also evaluated. (author)

  1. Chemical composition and antimicrobial activity of the essential oil from Aeolanthus suaveolens Mart. ex Spreng

    Directory of Open Access Journals (Sweden)

    Euclésio Simionatto

    2007-01-01

    Full Text Available The essential oils from leaves (sample A and flowers (sample B of Aeolanthus suaveolens Mart. ex Spreng were obtained by hydrodistillation and analyzed by GC, GC-MS, and chiral phase gas chromatography (CPGC. Six compounds have been identified from the essential oils, representing ca 94.3 and 93% of the oils corresponding to samples A and B, respectively. The major constituents of samples A and B essential oils were respectively, linalool (34.2%/34.9%, (--massoialactone (25.9%/17.0% and (E-beta-farnesene (25.4%/29.1%. The enantiomeric distribution of the monoterpene linalool was established by analysis on heptakis- (6-O-methyl-2,3-di-O-pentyl-beta-cyclodextrin capillary column. The antimicrobial activity of the essential oil from leaves and isolated compounds was also evaluated.

  2. Composition and evaluation of the antimicrobial activity of the essential oil of Senecio selloi Spreng DC.

    Directory of Open Access Journals (Sweden)

    G.N.S. Silva

    2013-01-01

    Full Text Available The essential oil of the aerial parts of Senecio selloi Spreng. DC. was extracted by hydrodistillation and analyzed by GC/MS. Nineteen compounds were identified, representing 99.9% of the total. The main compounds were found to be sesquiterpene hydrocarbons (71.3%, most of them with a bisabolane skeleton (59.4%. The major constituent was α-zingiberene (54%, followed by monoterpene α-isolimonene (16%. The essential oil was also tested against two Gram-positive and two Gram-negative bacterial species, three yeasts, and an algae. From the strains assayed, only Bacillus subtilis ATCC 6633 showed susceptibility (MIC and MBC = 4400 µg/mL to the essential oil.

  3. Distribution and status of Vicia menziesii Spreng. (Leguminosae): Hawaii's first officially listed endangered plant species

    Science.gov (United States)

    Warshauer, F.R.; Jacobi, J.D.

    1982-01-01

    Vicia menziesii Spreng., Hawai'i's first officially listed endangered plant species, formerly occurred across a large area in the upper montane-mesic forest habitat on the windward side of the island of Hawai'i. Until this species was `rediscovered? in 1974, it had last been seen in 1915, and it was presumed to be extinct. The population is presently thought to number 150?300 plants, most of which are seedlings. These are located within a 200 ha area on the eastern flank of Mauna Loa volcano. The primary factors responsible for the decline of V. menziesii are habitat loss and excessive predation on the plants by introduced ungulates. Continued logging and cattle grazing within its remnant range are major threats to its existence. Enhancing the survival of V. menziesii may best be accomplished by stabilizing its remaining habitat and allowing the population to reestablish itself naturally.

  4. Penetapan Kadar Mineral Besi, Magnesium Dan Seng Pada Daun Bangun-Bangun (Plectranthus Amboinicus L.) Segar Dan Yang Direbus Secara Spektrofotometri Serapan Atom

    OpenAIRE

    Pasaribu, Jusia Marluga

    2015-01-01

    Bangun-bangun leaf (Plectranthus amboinicus L.) is a plant that can use as a herb traditional in Indonesia. Batak women who are breastfeeding in Simalungun District, North Sumatera Province, has a tradition of consuming Bangun-bangun leaf in the form of vegetable soap for a month after born. They belive that by consuming the Bangun-bangun leaf soup, milk production will be increase. This Bangun-bangun leaf contain nutrient, especially iron, calsium, zinc, and magnesium. After observation fr...

  5. Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour

    Institute of Scientific and Technical Information of China (English)

    Sourov Kuri; Md Mustahsan Billah; S M Masud Rana; Zannatul Naim; Md Mahmodul Islam; Md Hasanuzzaman; Md Ramjan Ali; Rana Banik

    2014-01-01

    Objective: To study the phytochemical and biological properties (antioxidant, anthelmintic and thrombolytic) of methanolic extracts of Enhydra fluctuans Lour., a plant belonging to the Asteraceae family. Methods:The phytochemical evaluation was carried out by qualitative analysis. In vitro antioxidant activity of extract was studied using free radical scavenging assay, ability of reduction, total phenol and total flavonoid contents determination assays. The anthelmintic activity was determined using paralysis and death time of Pheretima posthuma (earthworm) and thrombolytic activity by clot disruption assay. Results:The phytochemical evaluation showed significant presence of flavonoids, triterpenes,carbohydrate, reducing sugars, saponins, phenols, diterpenes, protein and tannin. The antioxidant activity was found significant [IC50=(135.20±0.56) µg/mL] as compared to ascorbic acid [(130.00±0.76) µg/mL]. The reducing power was increased with concentration. Total phenol and total flavonoid contents were (153.08±0.38) mg/mL and (172.04±0.56) mg/mL respectively. The paralysis and death time of earthworms for different concentrations of extract were determined and compared with albendazole. The results showed that 10 mg/mL of the crude extract had similar effect with albendazole. Additionally, the crude extract showed a concentration depended relationship with its anthelmintic property. The clot lysis activity of crude extract was compared to the standard streptokinase’s clot lysis (40.13%) activity and found significant (31%). Conclusions: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals.

  6. Analysis of total proteins in pollen of Humulus scandens Lour in Wuhan Region of China by two-dimensional electrophoresis

    Institute of Scientific and Technical Information of China (English)

    LI Dongdong; HE Shaoheng

    2007-01-01

    Total proteins in the pollen of Humulus scandens Lour,one of the most popular aeroallergens in China,were analyzed by two-dimensional electrophoresis in the current study.The proteins were extracted by Trichloracetic acid (TCA) method,and then separated by isoelectric focusing as the first dimension and SDS-PAGE as the second dimension.The spots of proteins were visualized by staining with Coomassie Brilliant Blue.After analysis with software (ImageMaster 2D),122 different proteins were detected;isoelectric point (pI),Molecular weight (MW) and relativevolume of each protein in the pollen were also discovered.This is the first high-resolution,two-dimensional protein map of the pollen ofHumulus scandens Lour in China.Our finding has built a solid foundation for identification,characterization,gene cloning and standardization of allergenic proteins in the pollen ofHumulus scandens Lour for further studies.

  7. Changes of cell microstructure during development and senescence in perianth of Michelia figo (Lour.)Spreng.%含笑花被片发育和衰老过程中细胞超微结构的变化

    Institute of Scientific and Technical Information of China (English)

    郭素枝; 高华娟; 王湘平

    2009-01-01

    Dynamic changes of cell microstructure during development and senescence in perianth of Michelia . figo were studied. Experimental study of histochemistry showed that there were starch-similar compounds in ordinary parenchyma cells of perianth of M. figo and starch-similar compounds varied in numer dynamically during development and senescence in perianth of M. figo. There were very few starch-similar compounds and few chloroplasts contained at stage I in ordinary parenchyma cells of perianth of M. figo, mitochondria and lysosome were increased at stage Ⅱ and Ⅲ in perianth cells with bud growth and development. Perianth at stage Ⅳ had unfolded and starch-similar compounds gradually decreased and disappeared, mitochondria and lysosome in cells disintegrated in different degree. When perianth withered at stage Ⅴ , starch-similar compounds were less, cell wall seriously bended and deformed. Epidermis cells were tight and smooth at stage Ⅰ by SEM. Ornamentation on Epidermis cells was distinct and the quantity of starch-similar compounds in ordinary parenchyma cells were more and more with the growth and development of the perianth at stage Ⅱ and stage Ⅲ . When the perianth unfolded at stage Ⅳ , starch-similar compounds were discovered on ornamentation and interspaces of epidermis cells and stomata's orifice, but starch-similar compounds in cells gradually decreased and disappeared. When perianth withered at stage Ⅴ , epidermis cells collapsed, gaps appeared between epidermis cells, starch-similar compounds in cells disappeared and some flocculent substances were left. The relationship between cell microstructure changes and senescence in perianth of M. figo was discussed.%以含笑花被片为材料,研究了含笑花发育和衰老进程中细胞超微结构的动态变化.组织化学实验表明,花被片薄壁细胞具有淀粉复合物,其数量多少在花被片发育和衰老过程中呈动态变化.在透射电镜下,I时期花被片基本薄壁组织细胞中淀粉复合物极少,可见少量叶绿体;随着花蕾的生长发育,Ⅱ时期至Ⅲ时期花被片基本薄壁组织细胞中淀粉复合物、线粒体、溶酶体等逐渐增多;Ⅳ时期花被片已展开,其淀粉复合物逐渐减少或消失,细胞中的线粒体、溶酶体等细胞器出现不同程度解体;V时期花被片萎凋时,淀粉复合物甚少,细胞壁严重弯曲、变形.在扫描电镜下,从顶面观察花被片表皮细胞,可见I时期的表皮细胞紧密、光滑;Ⅱ时期至Ⅲ时期花被片表皮细胞表面纹饰结构清晰,同时,基本薄壁组织细胞中颗粒状淀粉复合物随着花被片的发育其数量逐渐增多;Ⅳ时期花被片展开时,表皮细胞垂周壁的条纹上、表皮细胞间隙、气孔孔口可见淀粉复合物,细胞中淀粉复合物逐渐减少且消失;V时期花被片已萎凋,表皮细胞塌陷、细胞间隙出现裂痕,细胞中淀粉复合物消失,有少量棉絮状物质.对含笑花被片细胞超微结构的变动与衰老的关系进行了讨论.

  8. Chemical Fingerprinting of the Fragrant Volatiles of Nineteen Indian Cultivars of Cymbopogon Spreng. (Poaceae

    Directory of Open Access Journals (Sweden)

    Rajendra C. Padalia

    2011-01-01

    Full Text Available The essential oil compositions of total nineteen cultivars of Cymbopogon Spreng. (Poaceae species viz. C. martinii (Roxb. Wats. var. motia Burk., C. flexuosus Nees ex Steud, C. winterinus Jowitt., C. pendulus Nees ex Steud. and a hybrid of C. khasianus (Hack Stapf. ex Bor and C. pendulus Nees ex Steud. were examined and compared using capillary GC and GC-MS . The analysis led to the identification of 48 constituents forming 90.1% to 99.7% of their total oil compositions with monoterpenoids (78.9% to 97.4% as the most exclusive constituents. The comparative results showed considerable variation in the qualitative and quantitative compositions of essential oils from nineteen different cultivars of the studied Cymbopogon species. On the basis of chemical similarity the cultivars of genus Cymbopogon was divided into five chemical variants/groups within two series viz. Citrati and Rusae. The volatile profile of existing cultivars of Cymbopogon are useful for their commercial utilization as they possess range of essential oils and aroma chemicals used in perfumery, flavour, pharmaceutical and other allied industries. Moreover, the marker constituents in their essential oils may be utilized as an important tool in oil authentication.

  9. Efeitos do sombreamento na anatomia foliar de Gallesia integrifolia (Spreng Harms e Schinnus terebinthifolius Raddi

    Directory of Open Access Journals (Sweden)

    M.S. Santos

    2014-03-01

    Full Text Available Realizou-se estudo com o objetivo de analisar as alterações na anatomia foliar de Gallesia integrifolia (Spreng Harms e Schinnus terebinthifolius Raddi quando cultivadas em ambientes de sombra moderada ou densa, simulando as condições naturais encontradas em sistemas agroflorestais tradicionais do sul da Bahia, Brasil. Plantas das duas espécies, com aproximadamente um ano de idade, foram cultivadas em casa de vegetação sob quatro níveis de sombreamento (25%, 17%, 10% e 5%. Estudos anatômicos do limbo foliar foram realizados a partir de material incluído em parafina e seccionado em micrótomo rotativo. Os diferentes níveis de sombreamento ocasionaram alterações na estrutura do mesofilo de ambas as espécies, com diferenças significativas na espessura do parênquima paliçádico, limbo foliar, e densidade estomática. Nas condições em que o experimento foi realizado os resultados obtidos indicaram que G. integrifolia apresenta maior capacidade de aclimatação a ambientes de sombra moderada e densa do que S. terebinthifolius, sendo mais indicada para o cultivo em sistemas agroflorestais pré-estabelecidos.

  10. VOLUME INCREMENT OF Nectandra megapotamica (Spreng. MEZ ON A MIXED RAIN FOREST

    Directory of Open Access Journals (Sweden)

    Luis Henrique da Silva Souza

    2009-10-01

    Full Text Available In this work, it is studied the growth of Nectandra megapotamica (Spreng. Mez species through a sampler compounded by eleven dominant trees selected from a rainforest in Nova Prata, Rio Grande do Sul. The mathematical model which better represents the growth trend in percentage volume was described as iv%=(b0+b1.ln d2, fitting individually per tree, being possible to describe the variations of slope (b1 and intercept (b0 coefficients between trees with the relation height – diameter (h/d, diameter increment measured on the last 5 cm on bore increment (id5 sampled on diameter breast height (d, exposition (EXPOS, corrected Basal Area Large (BALcor and crown length exposed to sunlight (Lc, considering the following expressions: b1= 0,41739 + 4,16179.id5 - 6,29332.h/d + 0,37823.EXPOS - 0,11519Ir + 5,80419.BALcor – 0,06858.Lc, with determination coefficient equal to 0,9979 and standard error in percentage of the mean of –1,73%, and b0= 2,62466 –13,37024.id5 + 22,08329.h/d – 1,33161.EXPOS + 0,34689.Ir –23,88899.BALcor + 0,25692.L, with determination coefficient fitting on 0,9907 and standard error in percentage of the mean of 2,71%.

  11. Predicting the Potential Invasive Distribution for Eupatorium adenophorum Spreng. in China%紫茎泽兰Eupatorium adenophorum Spreng.在中国入侵分布预测

    Institute of Scientific and Technical Information of China (English)

    MonicaPapes; A.Townsendpeterson

    2003-01-01

    Originating in Mexico, Eupatorium adenophorum Spreng., is a noxious weed long present as a non-native species in India, New Zealand, and Australia. In China, it is spreading rapidly, particularly in the southern and southwestern parts of the country, with serious economic and social consequences. A new method, ecological niche modeling, was applied for predicting its potential geographic range of invasion. Predicted potential distributional areas included the Chinese provinces where the plant is known to occur, as well as additional areas in central and eastern China that appear susceptible to further spread of this species.%原产于墨西哥的紫茎泽兰Eupatorium adenophorum Spreng.作为一个有害的外来物种在印度、新西兰和澳大利亚生长已有很长时间.在中国,尤其是在南方和西南地区其蔓延速度之快,带来了不可忽视的经济和社会后果.我们采用了生态位模拟新方法来预测紫茎泽兰可能入侵的范围.据此,预测的潜在分布区包括该植物在中国境内已分布的省份及未来华中、华东易受入侵的区域.

  12. Assessing the Chemical Composition and Antimicrobial Activity of Essential Oils from Brazilian Plants-Eremanthus erythropappus (Asteraceae), Plectrantuns barbatus, and P. amboinicus (Lamiaceae).

    Science.gov (United States)

    Santos, Nara O Dos; Mariane, Bruna; Lago, João Henrique G; Sartorelli, Patricia; Rosa, Welton; Soares, Marisi G; da Silva, Adalberto M; Lorenzi, Harri; Vallim, Marcelo A; Pascon, Renata C

    2015-01-01

    The chemical composition and antimicrobial activity of essential oils obtained from three Brazilian plant species-leaves and branches of Eremanthus erythropappus (Asteraceae), leaves of Plectranthus barbatus, and leaves of P. amboinicus (Lamiaceae)-were determined. Analysis by GC/MS and determination of Kovats indexes both indicated δ-elemene (leaves-42.61% and branches-23.41%) as well as (-)-α-bisabolol (leaves-24.80% and stem bark-66.16%) as major constituents of E. erythropappus essential oils. The main components of leaves of P. barbatus were identified as (Z)-caryophyllene (17.98%), germacrene D (17.35%), and viridiflorol (14.13%); whereas those of leaves of P. amboinicus were characterized as p-cymene (12.01%), γ-terpinene (14.74%), carvacrol (37.70%), and (Z)-caryophyllene (14.07%). The antimicrobial activity against yeasts and bacteria was assessed in broth microdilution assays to determine the minimum inhibitory concentration (MIC) necessary to inhibit microbial growth. In addition, the crude oil of branches of E. erythropappus was subjected to chromatographic separation procedures to afford purified (-)-α-bisabolol. This compound displayed biological activity against pathogenic yeasts, thus suggesting that the antimicrobial effect observed with crude oils of E. erythropappus leaves and branches may be related to the occurrence of (-)-α-bisabolol as their main component. Our results showed that crude oils of Brazilian plants, specifically E. erythropappus, P. barbatus, and P. amboinicus and its components, could be used as a tool for the developing novel and more efficacious antimicrobial agents. PMID:25970043

  13. Assessing the Chemical Composition and Antimicrobial Activity of Essential Oils from Brazilian Plants—Eremanthus erythropappus (Asteraceae, Plectrantuns barbatus, and P. amboinicus (Lamiaceae

    Directory of Open Access Journals (Sweden)

    Nara O. dos Santos

    2015-05-01

    Full Text Available The chemical composition and antimicrobial activity of essential oils obtained from three Brazilian plant species—leaves and branches of Eremanthus erythropappus (Asteraceae, leaves of Plectranthus barbatus, and leaves of P. amboinicus (Lamiaceae—were determined. Analysis by GC/MS and determination of Kovats indexes both indicated δ-elemene (leaves—42.61% and branches—23.41% as well as (−-α-bisabolol (leaves—24.80% and stem bark—66.16% as major constituents of E. erythropappus essential oils. The main components of leaves of P. barbatus were identified as (Z-caryophyllene (17.98%, germacrene D (17.35%, and viridiflorol (14.13%; whereas those of leaves of P. amboinicus were characterized as p-cymene (12.01%, γ-terpinene (14.74%, carvacrol (37.70%, and (Z-caryophyllene (14.07%. The antimicrobial activity against yeasts and bacteria was assessed in broth microdilution assays to determine the minimum inhibitory concentration (MIC necessary to inhibit microbial growth. In addition, the crude oil of branches of E. erythropappus was subjected to chromatographic separation procedures to afford purified (−-α-bisabolol. This compound displayed biological activity against pathogenic yeasts, thus suggesting that the antimicrobial effect observed with crude oils of E. erythropappus leaves and branches may be related to the occurrence of (−-α-bisabolol as their main component. Our results showed that crude oils of Brazilian plants, specifically E. erythropappus, P. barbatus, and P. amboinicus and its components, could be used as a tool for the developing novel and more efficacious antimicrobial agents.

  14. Antibakterijski učinak ekstrakta lista biljke Plectranthus amboinicus Benth u uzorcima voća, povrća i pilećeg mesa

    OpenAIRE

    Kumar Gupta, Sandeep; Singh Negi, Pradeep

    2016-01-01

    U radu je ispitan učinak ekstrakata lišća drevne indijske ljekovite biljke Plectranthus amboinicus Benth, izoliranih pomoću acetona i etilnog acetata, na sprečavanje rasta bakterija uzročnika kvarenja inokuliranih u uzorke svježeg kupusa i papaje, te na prirodnu mikrofloru pilećeg mesa. Dobiveni su ekstrakti uspješno smanjili broj bakterija u svim uzorcima hrane, no njihova je učinkovitost ovisila o složenosti sastava uzorka, pa je najveća bila u kupusu, zatim u papaji, a najmanja u mesu. Isp...

  15. Assessing the Chemical Composition and Antimicrobial Activity of Essential Oils from Brazilian Plants—Eremanthus erythropappus (Asteraceae), Plectrantuns barbatus, and P. amboinicus (Lamiaceae)

    OpenAIRE

    Nara O. dos Santos; Bruna Mariane; João Henrique G. Lago; Patricia Sartorelli; Welton Rosa; Marisi G. Soares; Adalberto M. da Silva; Harri Lorenzi; Vallim, Marcelo A.; Pascon, Renata C.

    2015-01-01

    The chemical composition and antimicrobial activity of essential oils obtained from three Brazilian plant species—leaves and branches of Eremanthus erythropappus (Asteraceae), leaves of Plectranthus barbatus, and leaves of P. amboinicus (Lamiaceae)—were determined. Analysis by GC/MS and determination of Kovats indexes both indicated δ-elemene (leaves—42.61% and branches—23.41%) as well as (−)-α-bisabolol (leaves—24.80% and stem bark—66.16%) as major constituents of E. erythropappus essential ...

  16. ANTIDIABETIC ACTIVITY OF AQUEOUS EXTRACT OF ROTULA AQUATICA LOUR. ROOTS IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

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    Ashwini C.S.

    2012-06-01

    Full Text Available Rotula aquatica (Lour. family-Boraginaceae roots are used for the treatment of diabetes traditionally. The aim of the present study was undertaken to investigate the hypoglycaemic and antidiabetic effect of single and repeated oral administration of the aqueous extract in normal and streptozotocin induced diabetic rats respectively, serum lipid profile was also examined. The dose of 200, 300 and 500 mg/kg body weight p.o. of the aqueous extract were evaluated and the dose of 200 mg/kg was identified as the most effective which reduced blood glucose level upto 40.6% after 4 hr of administration in normal rats and a fall of 38% in blood glucose level within 1 hr during glucose tolerance test (GTT in mild diabetic rats which has almost similar effect as that of standard drug glibenclamide (3 mg/kg bw. Severe diabetic rats were treated daily with 200 mg/kg bw for 14 days and a significant reduction of 56% was observed in fasting blood glucose level respectively, urine sugar level was decreasing with increasing in body weight and total cholesterol (TC, low density lipoprotein (LDL, triglyceride (TG levels were decreased by 24, 59 and 41% respectively, in severely diabetic rats whereas, cardioprotective, high density lipoprotein (HDL was increased by 26%. The data showed that aqueous extract has a remarkable hypoglycaemic, antidiabetic and hypolipidemic effect.

  17. The response of epiphytic bacteria on Vallisneria natans (Lour.) Hara (Hydrocharitaceae) to increasing nutrient loadings.

    Science.gov (United States)

    Cai, Xianlei; Yao, Ling; Gao, Guang; Xie, Yinfeng; Zhang, Yingying; Tang, Xiangming

    2016-06-01

    To investigate the effects of water column nutrient loading on epiphytic bacteria, we determined the abundance and community composition of epiphytic bacteria on the submerged macrophyte Vallisneria natans (Lour.) Hara during the growth season (June-October) under four different nutrient concentrations (nitrogen (N)-phosphorus (P) in mg L(-1) : 0.5-0.05, 1.0-0.1, 5.0-0.5, 10.0-1.0; hereafter NP-1, NP-2, NP-3, NP-4, respectively), using epifluorescence microscopy method and terminal restriction fragment length polymorphism (T-RFLP) analysis, respectively. Relative to low nutrient conditions (NP-1), there was no significant effect on the epiphytic bacterial community, and even a decrease in the number of epiphytic bacteria, which linked to the well growth status of host macrophytes at moderate nutrient conditions (NP-2). However, further nutrient enrichment induced significant increase in the abundance of epiphytic bacteria, and marked changes in the community structures of epiphytic bacteria. Furthermore, at high nutrient conditions, epiphytic bacterial communities varied widely temporally, and were not stable compared with those at the lower nutrient conditions. These results indicated that the effects of nutrient enrichment on epiphytic bacteria were nonlinear and dependent on the nutrient concentrations in the water. PMID:27143295

  18. The anti-cancer property of proteins extracted from Gynura procumbens (Lour. Merr.

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    Chaw-Sen Hew

    Full Text Available Gynura procumbens (Lour. Merr. belongs to the Asteraceae Family. The plant is a well-known traditional herb in South East Asia and it is widely used to treat inflammation, kidney discomfort, high cholesterol level, diabetic, cancer and high blood pressure. Our earlier study showed the presence of valuable plant defense proteins, such as peroxidase, thaumatin-like proteins and miraculin in the leaf of G. procumbens. However, the effects of these defense proteins on cancers have never been determined previously. In the present study, we investigated the bioactivity of gel filtration fractionated proteins of G. procumbens leaf extract. The active protein fraction, SN-F11/12, was found to inhibit the growth of a breast cancer cell line, MDA-MB-231, at an EC50 value of 3.8 µg/mL. The mRNA expressions of proliferation markers, Ki67 and PCNA, were reduced significantly in the MDA-MB-23 cells treated with SN-F11/12. The expression of invasion marker, CCL2, was also found reduced in the treated MDA-MB-231 cells. All these findings highlight the anti-cancer property of SN-F11/12, therefore, the proteins in this fraction can be a potential chemotherapeutic agent for breast cancer treatment.

  19. Câmara Municipal de Loures: uma experiência em arquitetura paisagista no sector público

    OpenAIRE

    Pereira, Mariana Matos da Silva

    2016-01-01

    O presente relatório de estágio reflete o trabalho realizado e a experiência adquirida ao longo de seis meses na Câmara Municipal de Loures. Os trabalhos desenvolvidos correspondem, sobretudo, ao que era pretendido por parte da Câmara Municipal tendo sido aplicados, em parte, os conhecimentos adquiridos ao longo da licenciatura e do mestrado em Arquitetura Paisagista na Universidade de Évora. Este relatório incide sobre a regulamentação do mobiliário urbano em todo o Município de ...

  20. 桂花花朵香气成分的研究进展%Research Progress of Flavoring Compositions in Osmanthus Fragrans Lour.Flower

    Institute of Scientific and Technical Information of China (English)

    施婷婷; 杨秀莲; 王良桂

    2014-01-01

    桂花花朵的香味由一系列低分子量的挥发性化合物组成,并形成其独有的特征。对桂花花朵香气成分的物质种类、影响因素进行了简单介绍,并对其提取与分析方法的研究与应用进展进行了综述。%Floral scent of Osmanthus fragrans Lour.is a complex mixture of low molecular weight volatile compounds,which gives O.fragrans Lour.flower unique fragrance.In this article,the types and influence fac-tors of flavoring compositions in O.fragrans Lour.flower are summarized,the research and development of ex-traction and analytical methods of flavoring compositions in O.fragrans Lour.flower are reviewed.

  1. Antioxidant activity, total phenolic, and total flavonoid of extracts from stems of Jasminum nervosum Lour

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    Wei, Xiangyong

    2011-06-01

    Full Text Available Guangxi traditional Chinese Medical University Universidad de Medicina Tradicional China de Guangxi This study evaluated the antioxidant activities of the extracts of Jasminum nervosum Lour. stems along with the effects of different extract solvents on total phenolics (TP, total flavonoids (TF, and antioxidant potential. The antioxidant activity of the extracts was assessed using the following methods: DPPH, ABTS+ both free radicals scavenging assays, and reducing assays. TP and TF were detected by spectrophotometric and HPLC methods. In former methods, the highest amount of TP content was ethy lacetate extract (EAE, expressed as gallic acid equivalents. The greatest TF content was in the n-butanol extract (BE, expressed as lutin equivalents. No significant difference was observed in the TP/TF content between these two extracts. The antioxidant activity and TP/TF content of three extracts seemed to follow the same trend. This implied that there is a good correlation between antioxidant activities and TP/TF content. But in HPLC methods, EAE contained the highest content of lutin and gallic acid, which decreased in the same order of EAE > BE > PE, the rank order of TP/TF content of EAE and BE were different according to antioxidant ability. The overall results showed that the EAE and BE were richer in phenolics and flavonoids than petroleum ether extract (PE, and may represent a good source of antioxidants.Este estudio evaluó las actividades antioxidantes de extractos de tallos de Jasminum nervosum Lour., y el efecto de diferentes disolventes de extracción en los fenoles totales (TP y flavonoides totales (TF, y su potencial antioxidante. La actividad antioxidante de los extractos fue evaluada usando los siguientes métodos: DPPH, ABTS+ y ensayos reductores. TP y TF fueron detectados por métodos espectroscópicos y por HPLC. Con el primer método, el contenido más alto de TP se obtuvo en el extracto con acetato de etilo (EAE, expresado como

  2. Cloning, expression, and characterization of pollen allergens from Humulus scandens (Lour) Merr and Ambrosia artemisiifolia L

    Institute of Scientific and Technical Information of China (English)

    Ai-lin TAO; Shao-heng HE

    2005-01-01

    Aim: To clone the pollen allergen genes in Humulus scandens (Lour) Merr (LüCao in Chinese) and short ragweed (Ambrosia artemisiifolia L) for recombinant allergen production and immunotherapy. Methods: The allergen genes were selectively amplified in the weed pollen cDNA pool by using a special PCR profile, with the primers designed by a modeling procedure. Following truncated gene cloning and confirmation of the pollen source, unknown 3'cDNA ends were identified by using the 3'-RACE method. The gene function conferred by the full-length coding region was evaluated by a homologue search in the GenBank database. Recombinant proteins expressed in Escherichia coli pET-44 RosettaBlue cells were subsequently characterized by N-terminal end sequencing, IgE binding, and crossreactivity. Results: Three full-length cDNAs were obtained in each weed. Multiple alignment analysis revealed that the deduced amino acid sequences were 83% identical to each other and 56%-90% identical to panallergen profilins from other species. Five recombinant proteins were abundantly expressed in nonfusion forms and were confirmed by using the N-terminal end sequence identity.Sera from patients who were allergic to A artemisiifolia reacted not only with rAmb a 8(D03) derived from A artemisiifolia, but also with recombinant protein rHum s 1(LCM9) derived from H scandens, which confirmed the allergenicity and cross-reactivity of the recombinant proteins from the 2 sources. Comparison of the degenerate primers used for truncated gene cloning with the full-length cDNA demonstrated that alternative nucleotide degeneracy occurred. Conclusion: This study demonstrates a useful method for cloning homologous allergen genes across different species, particularly for little-studied species. The recombinant allergens obtained might be useful for the immunotherapeutic treatment of H scandens and/or A artemisiifolia pollen allergies.

  3. Anticarcinogenesis effect of Gynura procumbens (Lour Merr on tongue carcinogenesis in 4NQO-induced rat

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    D. Agustina

    2006-09-01

    Full Text Available In Indonesia Gynura procumbens (Lour Merr leaves have been long used as various cancers medication. Many in vitro and in vivo studies have demonstrated anticarcinogenesis of ethanol extract of Gynura procumbens leaves. The aim of this study was to investigate the anticarcinogenesis of the ethanol extract of Gynura procumbens leaves on 4 nitroquinoline 1-oxide (4NQO-induced rat tongue carcinogenesis. Fifty six 4 week old male Sprague Dawley rats were used in this study and divided into 7 groups. Group 1, 2 and 3 were lingually induced by 4NQO for 8 weeks. In groups 2 and 3 the extract was given simultaneously with or after 4NQO induction finished, each for 10 weeks and 26 weeks, respectively. Groups 4, 5 and 6 were induced by 4NQO for 16 weeks. However, in groups 5 and 6 the extract was given as well simultaneously with or after the 4NQO induction, each for 18 weeks, respectively. Group 7 served as the as untreated control group. The results from microscopical assessment showed that tongue squamous cell carcinomas (SCC developed in 100% (3/3 of group 1. However, only 33.3% (2/6 and 25% (2/8 of rats in groups 2 and 3, respectively demonstrated tongue SCC. Among groups 4, 5 and 6, no significant difference of tongue SCC incidence was observed. From these results it is apparent that the ethanol extract of Gynura procumbens leaves could inhibit the progression of 4NQOinduced rat tongue carcinogenesis in the initiation phase.

  4. Effect ofBuchanania lanzan Spreng. bark extract on cyclophosphamide induced genotoxicity and oxidative stress in mice

    Institute of Scientific and Technical Information of China (English)

    Ritesh Jain; Sanmati Kumar Jain

    2012-01-01

    Objective:To elucidate the effect of ethanolic extract ofBuchanania lanzan Spreng. (B. lanan) bark against cyclophosphamide induced genotoxicity and oxidative stress in mice.Methods:The prevalence of micronuclei in bone marrow, the extent of lipid peroxidation, reduced glutathione and the status of the antioxidant enzymes, superoxide dismutase and catalase in liver of mice were used as intermediate biomarkers for chemoprotection. Lipid peroxidation and associated compromised antioxidant defenses in cyclophosphamide treated mice were observed in the liver.Results: Pre-treatment withB. lanzan250, 500 and1 000mg/ kg,p.o., daily for7 days significantly reduced the chromosomal damage and lipid peroxidation with concomitant changes in antioxidants and detoxification systems.Conclusions: These results point out the presence of chemopreventive phytoconstituents in the crude extract offering protection against cyclophosphamide induced genotoxicity and oxidative stress in mice.

  5. Complete Chloroplast Genome Sequence of Aquilaria sinensis (Lour. Gilg and the Evolution Analysis within the Malvalesorder

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    Ying eWang

    2016-03-01

    Full Text Available Aquilaria sinensis (Lour. Gilg is an important medicinal woody plant producing agarwood, which is widely used in traditional Chinese medicine. High-throughput sequencing of chloroplast (cp genomes enhanced the understanding about evolutionary relationships within plant families. In this study, we determined the complete cp genome sequences for A. sinensis. The size of the A.sinensis cp genome was 159,565 bp. This genome included a large single-copy region of 87,482 bp, a small single-copy region of 19,857 bp, and a pair of inverted repeats (IRa and IRb of 26,113 bp each. The GC content of the genome was 37.11%. The A.sinensis cp genome encoded 113 functional genes, including 82 protein-coding genes, 27 tRNA genes, and 4 rRNA genes. Seven genes were duplicated in the protein-coding genes, whereas 11 genes were duplicated in the RNA genes. A total of 45 polymorphic simple-sequence repeat loci and 60 pairs of large repeats were identified. Most simple-sequence repeats were located in the noncoding sections of the large single-copy/small single-copy region and exhibited high A/T content. Moreover, 33 pairs of large repeat sequences were located in the protein-coding genes, whereas 27 pairs were located in the intergenic regions. Aquilaria sinensis cp genome bias ended with A/T on the basis of codon usage. The distribution of codon usage in A.sinensis cp genome was most similar to that in the Gonystylus bancanus cp genome. Comparative results of 82 protein-coding genes from 29 species of cp genomes demonstrated that A.sinensis was a sister species to G. bancanus within the Malvales order. Aquilaria sinensis cp genome presented the highest sequence similarity of >90% with the G. bancanus cp genome by using CGView Comparison Tool. This finding strongly supports the placement of A.sinensis as a sister to G. bancanus within the Malvales order. The complete A.sinensis cp genome information will be highly beneficial for further studies on this traditional

  6. PERTUMBUHAN KALUS DAUN DEWA [Gynura procumbens (Lour Merr.] DENGAN KOMBINASI 2,4-DICHLOROPHENOXYACETIC ACID DAN KINETIN SECARA INVITRO

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    Samkhatin Khaniyah

    2012-09-01

    Full Text Available Tanaman daun dewa [Gynura procumbens (Lour Merr.] berguna untuk menurunkan kadar kolesterol darah, mengobati diabetes, tumor, dan sebagai obat anti kanker. Daun dewa mengandung senyawa metabolit sekunder yaitu flavonoid, saponin, dan minyak atsiri. Oleh karena itu, perlu adanya kultur in vitro yang diharapkan mampu meningkatkan jumlah metabolit sekunder, maka perlu dilakukan penelitian tentang induksi kalusdengan penambahan kombinasi 2,4-Dichlorophenoxyacetic acid dan kinetin yang tepat dapat digunakan untuk menginduksi kalus daun dewa. Tujuan penelitian ini untuk mengkaji pengaruh konsentrasi 2,4-D dan Kinetin serta interaksi keduanya terhadap induksi kalus daun dewa. Analisis menggunakan anava dua arah dan uji lanjut Duncan. Parameter yang diamati adalah parameter persentase eksplan yang hidup, persentase berkalus, berat basah, kering kalus, serta tekstur dan warna kalus . Dari Uji Jarak Berganda Duncan diperoleh hasil tertinggi pada konsentrasi 2,4-Dichlorophenoxyacetic acid 0.5 ppm dan konsentrasi kinetin 1 ppm sebesar 33.33% pada parameter persentase berkalus.Daun dewa [Gynura procumbens (Lour. Merr.] plant can be used to lower the blood cholesterol levels, to treat diabetes and tumors, and may be used as an anti-cancer drug. Daun dewa plant produces of secondary metabolites such as flavonoids, saponins, and essential oils. An in vitro culture is a necessity to increase the quantity of secondary metabolites using callus induction with the addition of a combination of 2,4-Dichlorophenoxyacetic acid and kinetin to induce daun dewa callus. The research will ecamine the influence of 2,4-Dichlorophenoxyacetic acid and Kinetin in various concentrations and their interaction on callus induction of daun dewa. Data were analyzed using advanced two-way anova and Duncan test. The test showed that the highest yeield was obtained from the combination of 0.5 ppm 2,4-D and 1 ppm kinetin, where 33.33% of plants had callus.

  7. Efeitos biológicos da Pfaffia glomerata (Spreng. Pedersen e da Pfaffia paniculata (Martius Kuntze (Amaranthaceae Biological effects of Pfaffia glomerata (Spreng. Pedersen and Pfaffia paniculata (Martius Kuntze (Amaranthaceae

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    Diógenes Aparício Garcia Cortez

    1999-07-01

    Full Text Available A Pfaffia glomerata (Spreng. Pedersen e da Pfaffia paniculata (Martius Kuntze (Amaranthaceae são plantas nativas da região alagada das ilhas de Porto Rico, Paraná, Brasil. São utilizadas na medicina popular como tônico, afrodisíaco e antidiabético. O extrato bruto metanólico, obtido das raízes das duas espécies, foi utilizado para avaliar a atividade moluscicida de caramujos (Biomphalaria glabrata. Além disso, o potencial hipoglicemiante da Pfaffia glomerata foi investigado em ratos tratados com aloxana. As saponinas do extrato bruto metanólico da P. glomerata e da P. paniculata foram analisadas em cromatografia em camada delgada e foi determinado o índice hemolítico. O extrato bruto metanólico obtido da Pfaffia glomerata apresentou maior conteúdo de saponinas hemolíticas e maior atividade moluscicida em relação à Pfaffia paniculata. Todavia, o tratamento com extrato de Pfaffia glomerata não promoveu efeitos significativos sobre os níveis glicêmicos.Pfaffia glomerata (Spreng. Pedersen. (Amaranthaceae and Pfaffia paniculata (Martius Kuntze are native plants found in the flooded area of the islands of Porto Rico, state of Paraná, Brazil. They are used in folk medicine as tonics, aphrodisiacs, and to treat diabetes. The methanolic extract obtained from roots of both species was used to evaluate the molluscicidal activity against the periwinkle Biomphalaria glabrata and the hypoglycemic activity of Pfaffia glomerata was investigated in rats with diabetes induced by alloxan. The saponins from the methanolic extract of P. glomerata and P. paniculata were analyzed by thin-layer chromatography and scum and the hemolitic index was measured. The methanolic extract obtained from Pfaffia glomerata showed higher content of hemolytic saponin than Pfaffia paniculata and presented molluscicidal activity against Biomphalaria glabrata from 200 ppm. However, the treatment with P. glomerata extract did not promote any significant effect on

  8. Benzilaminopurina (BAP e thidiazuron (TDZ na propagação in vitro de Pfaffia glomerata (Spreng. Pedersen Benzylaminopurine (BAP and thidiazuron (TDZ on in vitro propagation of Pfaffia glomerata (Spreng. Pedersen

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    R. Flores

    2009-01-01

    Full Text Available Pfaffia glomerata (Spreng. Pedersen, conhecida como ginseng brasileiro, é muito utilizada pela medicina popular devido suas propriedades fitoterápicas. Este trabalho teve como objetivo avaliar o efeito de concentrações de BAP e de TDZ na propagação in vitro de dois acessos (BRA e JB-UFSM desta espécie. Segmentos nodais, provenientes de plantas assépticas, foram cultivados em meio Murashige e Skoog (MS suplementado com 0, 1 e 5 μM de BAP ou TDZ. Após 30 dias, as plantas foram transferidas para meio MS não suplementado com citocinina. Observou-se que a organogênese in vitro de P. glomerata é genótipo-dependente. O acesso BRA mostrou um maior potencial para a propagação in vitro em relação ao JB-UFSM. O cultivo dos segmentos nodais do acesso BRA em meio com 1 μM de TDZ, seguido pelo subcultivo dos brotos para meio isento de citocininas mostrou ser um método viável para a propagação in vitro devido à alta taxa de multiplicação e o bom desenvolvimento das plantas. No acesso JB-UFSM, o cultivo dos segmentos nodais em meio não acrescido de citocinina mostrou ser o método mais adequado para a produção de um grande número de plantas com alta qualidade.The plant Pfaffia glomerata (Spreng. Pedersen, known as Brazilian ginseng, is extensively used in folk medicine due to its phytotherapic characteristics. This work aimed to evaluate the effect of BAP and TDZ concentrations on the in vitro propagation of two sources (BRA and JB-UFSM of this species. Nodal segments originated from aseptically grown plants were cultured on Murashige and Skoog (MS medium supplemented with 0, 1 and 5 μM BAP or TDZ. After 30 days, the plants were transferred to MS medium without cytokinin. The in vitro organogenesis of P. glomerata is genotype-dependent. The source BRA had greater potential for in vitro propagation than JB-UFSM. The culture of BRA nodal segments on medium with 1 μM TDZ, followed by subcultivation of sprouts on cytokinin

  9. Nitrogênio e fósforo no crescimento de plantas de ginseng brasileiro [Pfaffia glomerata(Spreng. Pedersen] cultivadas in vitro Nitrogen and phosphorus on growth of brazilian ginseng [Pfaffia glomerata (Spreng. Pedersen] in vitro cultured plants

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    Denise Russowski

    2003-02-01

    Full Text Available O ginseng brasileiro [Pfaffia glomerata (Spreng. Pedersen] apresenta propriedades medicinais marcantes e, por isso, atualmente, é largamente explorado de forma extrativista, tanto por parte dos coletores de ervas como pela indústria farmacêutica. Este trabalho objetivou caracterizar o efeito da variação isolada da concentração de N e P do meio MS no crescimento de plantas de P. glomerata cultivadas in vitro. Segmentos nodais de 1,0cm de comprimento e sem folhas, de plantas já estabelecidas in vitro, foram cultivados em meio MS contendo cinco concentrações (0, 25, 50, 100 e 150% da concentração padrão do meio de cultura MS de nitrogênio ou fósforo. Aos 15 dias após a inoculação (DAI, o número de raízes e o percentual de enraizamento são maiores na concentração de N e P equivalentes a 50% daquela do meio de cultura MS. Aos 40 DAI, o crescimento em altura das brotações, número de segmentos nodais, índice de área foliar, número de folhas, matéria seca de raízes, da parte aérea e total da planta é maior na concentração de N e P, em média, próxima a 80% daquela do meio de cultura MS.Brazilian ginseng [Pfaffia glomerata (Spreng. Pedersen] has expressionable medicinal properties, and therefore, nowadays it is extensively exploited either by the herb collectors as well as the pharmaceutical industry. The aim of this work was to characterize the effect of N and P concentrations from the MS medium on growth of P. glomerata in vitro cultured plants. 1-node segment without leaves, from in vitro cultivated plants, were grown on five levels (0, 25, 50, 100, and 150% of the strength of the MS medium of N and P. After 15 days of inoculation, root number and rooting percentage are greater on N and P levels of the MS medium with 50% strength. After 40 days of inoculation, growth, based on sprout height, number of nodal segments per plant, leave area index per plant, number of leaves per plant, dry weight of aerial parts, roots

  10. Evaluación de la toxicidad aguda de extractos de plantas medicinales por un método alternativo (Evaluation of acute toxicity of extracts of medicinal plants by an alternative testing

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    Deodelsy Bermúdez Toledo:

    2007-03-01

    Full Text Available Se evaluó la toxicidad a dosis única de seis extractos de plantas medicinales: Psidium guajava L. (guayaba, Eucalyptus citriodoraHooker (eucalipto de limón, Cymbopogon citratus (DC Stapf (caña santa, hierba de limón, Hibiscus elatus Sw. (majagua, Justicia pectoralis Jacq. (tilo, Plectranthus amboinicus (Lour. Spreng (orégano francés mediante un método alternativo e internacionalmente validado y aceptado (procedimiento de dosis fijas en ratasSprague Dawley. El grupo tratado recibió por vía oral una dosis límite de 2000 mg/kg de masa corporal y el control negativocloruro de sodio 0,9%. En las condiciones del ensayo no se produjo mortalidad ni se manifestaron síntomas indicativos de toxicidad en los animales. Los estudios anatomopatológicos macroscópicos nomostraron ninguna alteración en los órganos estudiados. La DL50 de los extractos de estudio se encuentra por encima de 2000mg/kg de masa corporal, calificándose éstos, según el Sistema Global Armonizado, como “No clasificadas” (”No tóxicas”.A single dose toxicity study in six extracts of medicinal plants: Psidium guajava L. (guava, Eucalyptus citriodora Hooker (lemon eucalyptus, Cymbopogon citratus (DC Stapf (lemon grass, Hibiscus elatus Sw. (majagua, Justicia pectoralis Jacq. (linden, lectranthus amboinicus (Lour. Spreng (French oregano was done, using an alternative internationally validated and accepted method (Fix Dose Procedure in Sprague Dawley rats. The treated group received by oral route a dose limit of 2000 mg/kg of corporal mass and the controlnegative chloride of sodium 0.9%. Under the assay conditions didn't produced mortality neither they showed indicative symptoms oftoxicity in the animals. The macroscopic anatomopathology studies didn't show any alteration in the studied organs. The LD50 ofthe study extracts is above 2000 mg/kg of corporal mass, being qualified these, according to the Harmonized Global System,as "Not classified" ("Not toxic".

  11. Ras-Related Nuclear Protein Ran3B Gene Is Involved in Hormone Responses in the Embryogenic Callus of Dimocarpus longan Lour.

    OpenAIRE

    Qilin Tian; Yuling Lin; Dongmin Zhang; Ruilian Lai; Zhongxiong Lai

    2016-01-01

    Ras-related guanosine triphosphate (GTP)-binding nuclear protein (Ran) GTPases function as molecular switches and regulate diverse cellular events in eukaryotes. Our previous work suggested that DlRan3B is active during longan (Dimocarpus longan Lour.) somatic embryogenesis (SE) processes. Herein, subcellular localization of DlRan3B was found to be localized in the nucleus and expression profiling of DlRan3B was performed during longan SE and after exposure to plant hormones (indoleacetic aci...

  12. Renoprotective Effects of Vitex megapotamica (Spreng. Moldenke in C57BL/6 LDLr-Null Mice Undergoing High Fat Diet

    Directory of Open Access Journals (Sweden)

    Valdinei de Oliveira Araújo

    2015-01-01

    Full Text Available Although Vitex megapotamica (Spreng. Moldenke is used in Brazilian folk medicine as hypolipidemic drug no study has been conducted to evaluate the effects of this species in an experimental model of atherosclerosis. So, the aim of this study was to evaluate the possible renoprotective activity of methanolic extract obtained from Vitex megapotamica (MEVM using C57BL/6 LDLr-null mice submitted to high fat diet (HFD. MEVM was orally administered at doses of 30, 100, and 300 mg/kg, for three weeks, starting from the 2nd week of HFD. Systolic blood pressure (SBP and diuretic activity were measured weekly. At the end of experiments the serum lipids, atherogenic index serum (AIS, oxidative stress, and markers of renal function were determined. HFD induced a significant increase in the systolic blood pressure, dyslipidemia, increase in AIS, and lipid peroxidation accompanied by an important reduction in renal function. Treatment with MEVM was able to prevent increase in SBP, total cholesterol, triglycerides, AIS, urea, and creatinine levels in LDLr-null mice. These effects were accompanied by a significant reduction in oxidative stress and renal injury. The data reported here support the potential of Vitex megapotamica as candidate to be an herbal medicine used in cardiovascular or renal diseases.

  13. Desenvolvimento de frutos de Longan (Dimocarpus longan lour na região de Jaboticabal-SP Development of Longan (Dimocarpus longan lour fruits in the region of Jaboticabal-SP, Brazil

    Directory of Open Access Journals (Sweden)

    Natanael de Jesus

    2008-03-01

    Full Text Available O presente estudo teve por objetivo avaliar o desenvolvimento de frutos de plantas de longan (Dimocarpus longan Lour na região de Jaboticabal, estabelecendo-se, portanto, a curva de crescimento dos frutos. As avaliações realizadas foram semanais, consistindo em medições do diâmetro longitudinal e transversal dos frutos marcados, a partir do momento da formação da polpa. Quinzenalmente, realizaram-se avaliações de características físicas: massa de fruto (g, casca (g, polpa (g semente (g, e químicas: acidez titulável (AT, sólidos solúveis (SS, ácido ascórbico (AA e ratio (SS/AT. Com os resultados obtidos, pode-se concluir que o ciclo de frutificação da Longan na região é entre 120 e 130 dias após antese, período em que o fruto estaria apto para o consumo. A curva do desenvolvimento dos frutos segue um padrão sigmoidal simples. Com relação aos atributos físicos e químicos, constatou-se que a planta 6 apresentou os melhores resultados, podendo ser recomendada como planta-matriz.This work was carried out to evaluate the development of longan (Dimocarpus longan Lour fruits in the region of Jaboticabal, and establish the fruit growth curve. Weekly evaluations were performed to measure longitudinal and transversal diameter of selected fruits after pulp formation. Every two weeks, physical and chemical parameters were evaluated: fruit, peel, pulp and seed mass (g; titrable acidity (TA, soluble solids (SS, ascorbic acid (AA and the ratio (SS/TA. Results indicate that longan reproductive cycle in the region is 120 to 130 days after anthesis, period after which the fruit is good for consumption, when the fruit development curve follows a simple sigmoidal pattern. Physical and chemical parameters have shown that the longan fruits analyzed here have a high consumption potential.

  14. Study on the Cytotoxic, Genotoxic and Clastogenic Potential of Attalea phalerata Mart. ex Spreng. Oil Pulp In Vitro and In Vivo Experimental Models

    Science.gov (United States)

    Lima Tolouei Menegati, Sara Emilia; Karenina Traesel, Giseli; Souza de Araújo, Flávio Henrique; Honaiser Lescano, Caroline; Moraes Peixoto, Sara; Mao Silva, Felipe Ariel; Heredia Vieira, Silvia Cristina; do Carmo Vieira, Maria; Oesterreich, Silvia Aparecida

    2016-01-01

    Attalea phalerata Mart. ex Spreng. (Arecaceae), popularly known as “bacuri”, is used in Brazilian folk medicine. Its oil is used orally to relieve pulmonary congestion and joint pain. In topical applications, it is applied as an effective hair tonic and anti-dandruff. The in natura pulp and its nuts are used as food because of its nutritional value. Despite its use in folk medicine, there is a lack of data regarding its in vivo/in vitro cytotoxic/genotoxic and clastogenic effects. Therefore, in this study, we evaluated the cytotoxic, genotoxic and clastogenic effects of Attalea phalerata Mart. ex Spreng. oil (APMO) in vitro and in vivo. For the analysis of cytotoxic potential, the Artemia salina and MTT (3-(4,5-dimethizzol-zyl)-2,5-diphenyltetrazolium bromide) assays were performed. Possible cytotoxic, genotoxic and clastogenic effects of APMO intake were determined by performing the comet and micronucleus assays. Male and female Wistar rats were orally treated with doses of 125, 250, 500 or 1000 mg.kg-1 of the APMO daily for 28 consecutive days (four weeks). The results showed that the APMO did not induce cell death in the experiments of Artemia salina and MTT, indicating that it has no cytotoxicity. The APMO did not cause significant damage to the DNA of the rats in the four doses used when compared to the negative control group (saline + Tween® 80). The APMO did not present any significant increase in micronucleated polychromatic erythrocytes (MNPCEs) for the four tested doses. When compared to the positive control group, all groups (comet and micronucleus tests) were statistically different. These data suggest that the administration of Attalea phalerata Mart oil. ex Spreng does not cause cytotoxicity, genotoxicity and clastogenicity in experimental models in vitro and in vivo following oral administration in this study. PMID:27764219

  15. 薯莨鞣质成分及生物活性的研究进展%Dioscorea Cirrhosae Lour Tannins Components and the Research Progress of Biological Activity

    Institute of Scientific and Technical Information of China (English)

    李忠军; 杨燕军; 孟巨光

    2015-01-01

    Studies have shown that Dioscorea cirrhosae Lour contains many biological active ingredient , the tannins is one of the most important effective components .Based on the recent more than ten years on Dioscorea cirrhosae Lour tannins of the latest research results , this paper expounds the Dioscorea cirrhosae Lour tannins chemical extraction process , types, medicinal, and the research progress of biological activity .%研究表明,薯莨含有大量的生物活性成分,其中鞣质是最重要有效成分之一。结合近10年来有关薯莨鞣质最新研究成果,阐述了薯莨鞣质化学成分提取工艺、种类、药用及生物活性的研究进展。

  16. Dioscorea Cirrhosae Lour Tannins Components and the Research Progress of Biological Activity%薯莨鞣质成分及生物活性的研究进展

    Institute of Scientific and Technical Information of China (English)

    李忠军; 杨燕军; 孟巨光

    2015-01-01

    Studies have shown that Dioscorea cirrhosae Lour contains many biological active ingredient , the tannins is one of the most important effective components .Based on the recent more than ten years on Dioscorea cirrhosae Lour tannins of the latest research results , this paper expounds the Dioscorea cirrhosae Lour tannins chemical extraction process , types, medicinal, and the research progress of biological activity .%研究表明,薯莨含有大量的生物活性成分,其中鞣质是最重要有效成分之一。结合近10年来有关薯莨鞣质最新研究成果,阐述了薯莨鞣质化学成分提取工艺、种类、药用及生物活性的研究进展。

  17. Produção de biomassa de Pfaffia glomerata (Spreng. Pedersen e Plantago major L. em cultivo solteiro e consorciado Pfaffia glomerata (Spreng. Pedersen and Plantago major L. biomass production in single culture and intercropped

    Directory of Open Access Journals (Sweden)

    Eduardo Xavier do Nascimento

    2007-06-01

    Full Text Available O objetivo do trabalho foi avaliar a capacidade produtiva da Pfaffia glomerata (Spreng. Pedersen e de Plantago major L., em cultivo solteiro e consorciado. Os tratamentos em estudo foram três e quatro fileiras de plantas de tansagem por canteiro, duas fileiras de fáfia espaçadas de 36 cm ou de 54 cm, três fileiras de tansagem alternadas com duas fileiras de fáfia (36 cm-T3F36 e quatro fileiras de tansagem alternadas com duas fileiras de fáfia (54 cm-T4F54. Foi utilizado o delineamento blocos casualizados, com quatro repetições. Os espaçamentos entre plantas foram de 40 cm para fáfia e de 30 cm para tansagem. O tipo de cultivo e os espaçamentos entre fileiras não influenciaram significativamente a produção de massa fresca e seca da parte aérea e das raízes da fáfia. Na tansagem foi observado efeito significativo da forma de cultivo em todas as características avaliadas e do número de fileiras para o número de pendões. As alturas máximas das plantas de fáfia foram de 185 cm e 183 cm, aos 172 e 164 dias após o transplante, sob cultivo solteiro, nos espaçamentos de 54 cm e 36 cm, respectivamente. A razão de área equivalente (RAE, para o consórcio T3F36 foi de 1,07 e para o T4F54 foi de 1,50. No caso de recomendação agronômica, optar-se-ía pelo consórcio T4F54.This study aimed to evaluate the production of Pfaffia glomerata (Spreng. Pedersen and Plantago major biomass in sole culture and intercropped. The treatments in study were three and four rows of plants of common plant for plat, two rows of Pfaffia glomerata spaced of 36 cm or 54 cm, three rows of common plant alternated with two rows of Pfaffia glomerata (36 cm-T3F3(6 and four alternated rows of common plant with two rows of Pfaffia glomerata (54 cm-T4F54. The experimental was desigm randomized blocks, with four replications. The spacing between plants was 40 cm for Pfaffia glomerata and 30 cm for common plant. The culture type and spacing between rows did not

  18. Assessment of haemolytic, cytotoxic and free radical scavenging activities of an underutilized fruit, Baccaurea ramiflora Lour. (Roxb.) Muell. Arg.

    Science.gov (United States)

    Saha, Manas Ranjan; Dey, Priyankar; Chaudhuri, Tapas Kumar; Goyal, Arvind Kr; Sarker, Dilip De; Sen, Arnab

    2016-02-01

    Baccaurea ramiflora Lour. (Roxb.) Muell. Arg. is an underutilized juicy fruit bearing plant found in sub-Himalayan area, South China, Indo-Burma region, etc. The fruit is considered to be nutritive, and in this study, we evaluated its antioxidant, haemolytic and cytotoxic properties. The juice was examined for the quenching activity of hydroxyl radical, nitric oxide, singlet oxygen, peroxynitrite, total antioxidant activity (TAA), erythrocyte membrane stabilizing activity (EMSA) along with quantification of phenolic and flavonoid contents and also tested for its potential activity as iron chelator, inhibitor of lipid peroxidation and total reducing power. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) were also performed to correlate antioxidant capacities with the phenolic and flavonoid content. Haemolytic activity on murine erythrocyte and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxic test was performed on murine splenocytes, thymocytes, hepatocytes and peritoneal exudates macrophage to examine the cytotoxic effect of its juice. The result exhibited its potent free radical scavenging activity. In case of TAA, DPPH (2, 2-diphenyl-1-picrylhydrazyl), EMSA and lipid peroxidation, the fruit juice was found to have significant (P cytotoxic test confirms that the juice does not contain any cytotoxic effect and the fruit is safe for consumption. Fourier transform infrared (FTIR) spectra analysis exhibited high possibility of presence of flavonoid compounds in the juice.

  19. Chemical constituents and antimicrobial properties of the essential oil and ethanol extract from the stem of Aglaia odorata Lour.

    Science.gov (United States)

    Joycharat, Nantiya; Thammavong, Sonesay; Voravuthikunchai, Supayang Piyawan; Plodpai, Patimaporn; Mitsuwan, Watcharapong; Limsuwan, Surasak; Subhadhirasakul, Sanan

    2014-01-01

    The stem-derived essential oil of Aglaia odorata Lour. was obtained by hydrodistillation using a Clevenger-type system. Gas chromatography-mass spectrometry analysis of the oil revealed the identification of 39 compounds, representing 76.4% of the oil; germacrene D (20.3%), α-humulene (17.1%), α-himachalene (12.7%) and β-caryophyllene (10.2%) were the major components. Ar-turmerone (1) and eichlerialactone (2) were isolated from the stem oil and ethanolic stem extract of this plant species, respectively. Antimicrobial activities of the oil and ethanol extract were tested against both Gram-positive and Gram-negative bacterial strains including Bacillus cereus ATCC 11778, Staphylococcus aureus ATCC 25923, Acinetobacter baumannii ATCC 19606 and Escherichia coli ATCC 25922, as well as three rice fungal pathogens Bipolaris oryzae, Pyricularia oryzae and Rhizoctonia solani using broth microdilution method. The oil and 1 exhibited significant antifungal activity against the three rice pathogens tested, whereas 2 exhibited good antibacterial activity against both the Gram-positive pathogens tested. PMID:24934340

  20. Opportunities for Phytoremediation and Bioindication of Arsenic Contaminated Water Using a Submerged Aquatic Plant:Vallisneria natans (lour.) Hara.

    Science.gov (United States)

    Chen, Guoliang; Liu, Xingmei; Brookes, Philip C; Xu, Jianming

    2015-01-01

    The identification of plants with high arsenic hyperaccumulating efficiency from water is required to ensure the successful application of phytoremediation technology. Five dominant submerged plant species (Vallisneria natans (Lour.) Hara., Potamageton crispus L., Myriophyllum spicatum L., Ceratophyllum demersum L. and Hydrilla verticillata (L.f.) Royle) in China were used to determine their potential to remove As from contaminated water. V. natans had the highest accumulation of As among them. The characteristics of As accumulation, transformation and the effect of phosphate on As accumulation in V. natans were then further studied. The growth of V. natans was not inhibited even when the As concentration reached 2.0 mg L(-1). After 21 d of As treatment, the bioconcentration factor (BCF) reached 1300. The As concentration in the environment and exposure time are major factors controlling the As concentration in V. natans. After being absorbed, As(V) is efficiently reduced to As(III) in plants. The synthesis of non-enzymic antioxidants may play an important role under As stress and increase As detoxication. In addition, As(V) uptake by V. natans was negatively correlated with phosphate (P) uptake when P was sufficiently supplied. As(V) is probably taken up via P transporters in V. natans. PMID:25397983

  1. Microcystin-LR induced oxidative stress and ultrastructural alterations in mesophyll cells of submerged macrophyte Vallisneria natans (Lour.) Hara.

    Science.gov (United States)

    Jiang, Jinlin; Gu, Xueyuan; Song, Rui; Wang, Xiaorong; Yang, Liuyan

    2011-06-15

    Microcystins produced by cyanobacteria in the aquatic environment are a potential risk to aquatic plants. In the present study, the uptake of microcystin-LR (MC-LR) and related physiological and biochemical effects on Vallisneria natans (Lour.) Hara were investigated at concentrations of 0.1-25.0 μg L(-1). Results showed that O(2)(-) intensity was significantly induced at 1.0 μg L(-1) and reached a maximum level at 5.0 μg L(-1). Superoxide dismutase (SOD) and peroxidase (POD) were induced with increasing MC-LR concentrations as an antioxidant response. Catalase (CAT) was significantly induced while GSH/GSSG (reduced/oxidized glutathione) ratio was significantly reduced at 0.1 μg L(-1). The induction of glutathione S-transferase (GST) and inhibition of GSH revealed that GSH was involved in the detoxification of MC-LR in plants. Oxidative damage was evidenced by the significant increase of malondialdehyde content at 1.0 μg L(-1). A pigment pattern change and a series of significant ultrastructural alterations were also observed due to MC-LR exposure. The lowest non-effect concentration of MC-LR for V. natans at the subcellular and molecular level is around 0.5 μg L(-1). These results imply that even at relatively low MC-LR concentrations the aquatic plants may still suffer a negative ecological impact. PMID:21466917

  2. Arsenic species uptake and subcellular distribution in Vallisneria natans (Lour.) Hara as influenced by aquatic pH.

    Science.gov (United States)

    Chen, Guoliang; Liu, Xingmei; Xu, Jianming; Brookes, Philip C; Wu, Jianjun

    2014-04-01

    Vallisneria natans (Lour.) Hara, a widely distributed submerged aquatic plant, is a promising species for arsenic (As) removal from contaminated water. We investigated the effects of pH on the accumulation, subcellular distribution and detoxification of As in V. natans. The results showed that the optimum pH for submerged V. natans growth is close to 7.0. The accumulation of As in the plant increased with the increase of pH (p < 0.05). This may have been due to arsenic/phosphate transporters with a higher affinity for the more highly electronegative AsO4 (3-) than for HAsO4 (2-) and H2AsO4 (-). After As(V) was accumulated by plants, more than 80 % was reduced to As(III), but As reduction decreased with increased pH. The majority of accumulated As and reduced As(III) (47 %-66 %) was found in the vacuoles. Higher As concentrations in vacuoles could be considered as an important mechanism for As detoxification in submerged plants. PMID:24420344

  3. Rosmarinic Acid, a New Polyphenol from Baccaurea ramiflora Lour. Leaf: A Probable Compound for Its Anti-Inflammatory Activity

    Directory of Open Access Journals (Sweden)

    Talambedu Usha

    2014-12-01

    Full Text Available Despite several pharmacological applications of Baccaurea ramiflora Lour., studies on the influence of its polyphenol content on pharmacological activity such as anti-inflammatory properties have been scarce. Here we evaluated in vitro antioxidant activity, poyphenolics by HPLC and the anti-inflammatory potential of the methanolic leaf extract of Baccaurea ramiflora (BME and its protective effects in carrageenan-induced paw edema model of inflammation in rats. The BME extract contained 79.06 ± 0.03 mg gallic acid equivalent (GAE/g total polyphenols, 28.80 ± 0.01 mg quercetin equivalent (QE/g flavonoid and 29.42 ± 0.01 μg cathechin equivalent/g proanthocyanidin respectively and rosmarinic acid (8 mg/kg as a main component was identified by HPLC. Results demonstrate that administration of BME at the dose of 200 mg/kg can reduce paw edema by over 63%, and it exhibits a dose-response effect. Depending on concentration, the extract exerted scavenging activity on DPPH radical (IC50 36.4 μg/mL, significantly inhibited IL-1β (4.4 pg/mg protein and TNF-α (0.21 ng/μg protein. Therefore, we conclude BME causes a substantial reduction of inflammation in in vivo models. We propose that rosmarinic acid and similar phenolic compounds may be useful in the therapy of inflammation-related injuries.

  4. Pharmacological studies on Buchanania lanzan Spreng.-A focus on wound healing with particular reference to anti-biofilm properties

    Institute of Scientific and Technical Information of China (English)

    Ashok Pattnaik; Ratul Sarkar; Amrita Sharma; Kirendra Kumar Yadav; Alekh Kumar; Paramita Roy; Avijit Mazumder; Sanmoy Karmakar; Tuhinadri Sen

    2013-01-01

    Objective:To evaluate the wound healing activity of the methanolic root extract of Buchanania lanzan Spreng. (B. lanzan), with a focus on antimicrobial and anti-biofilm properties. Methods: The extract was evaluated for its wound healing properties (excision and incision models) as evident from the analysis of tensile strength and wound contraction. The extract was also screened for antibacterial properties against different Gram positive and Gram negative bacterial strains. B. lanzan was also studied for its effect on biofilm formation and disruption of preformed biofilms. The synergistic effect of B. lanzan was determined in combination with gentamicin. Results:Topical application of B. lanzan (10%w/w ointment) significantly increased (40.84%) the tensile strength in the incision wound model. B. lanzan also showed significant wound healing activity in excision model and such significant activity was observed from the 9th day. Whereas Soframycin displayed significant wound healing activity from the 6th day. It was found that root extracts of B. lanzan revealed significant inhibition against all tested pathogens. B. lanzan displayed antimicrobial activity against Gram positive (MIC 0.625 mg/mL) and Gram negative (MIC 0.625-1.25 mg/mL). B. lanzan was able to reduce biofilm formation and also caused disruption of preformed biofilms in a manner similar to ciprofloxacin. However, gentamicin was found to be ineffective against biofilms formed by Gram negative organism. According to the fractional inhibitory concentration index, B. lanzan displayed synergistic activity when it was combined with gentamicin. Conclusions:From this study it may be concluded that the root extract of B. lanzan revealed significant wound healing potential, which was supported and well correlated with pronounced antibacterial activity of the tested plant parts.

  5. Selection of Reference Genes for Expression Study in Pulp and Seeds of Theobroma grandiflorum (Willd. ex Spreng.) Schum

    Science.gov (United States)

    Ferraz dos Santos, Lucas; Santana Silva, Raner José; Oliveira Jordão do Amaral, Daniel; Barbosa de Paula, Márcia Fabiana; Ludke Falcão, Loeni; Legavre, Thierry; Alves, Rafael Moyses

    2016-01-01

    Cupuassu (Theobroma grandiflorum [Willd. ex Spreng.] Schum) is a species of high economic importance in Brazil with great potential at international level due to the multiple uses of both its seeds and pulp in the industry of sweets and cosmetics. For this reason, the cupuassu breeding program focused on the selection of genotypes with high pulp and seed quality—selection associated with the understanding of the mechanisms involved in fruit formation. Gene expression is one of the most used approaches related to such understanding. In this sense, quantitative real-time PCR (qPCR) is a powerful tool, since it rapidly and reliably quantifies gene expression levels across different experimental conditions. The analysis by qPCR and the correct interpretation of data depend on signal normalization using reference genes, i.e. genes presenting a uniform pattern of expression in the analyzed samples. Here, we selected and analyzed the expression of five genes from cupuassu (ACP, ACT, GAPDH, MDH, TUB) to be used as candidates for reference genes on pulp and seed of young, maturing and mature cupuassu fruits. The evaluation of the gene expression stability was obtained using the NormFinder, geNorm and BestKeeper programs. In general, our results indicated that the GAPDH and MDH genes constituted the best combination as reference genes to analyze the expression of cupuassu samples. To our knowledge, this is the first report of reference gene definition in cupuassu, and these results will support subsequent analysis related to gene expression studies in cupuassu plants subjected to different biotic or abiotic conditions as well as serve as a tool for diversity analysis based on pulp and seed quality. PMID:27501324

  6. Plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methicillin resistant Staphylococcus aureus biofilm and blood sucking mosquito larvae

    Science.gov (United States)

    Vijayakumar, S.; Vinoj, G.; Malaikozhundan, B.; Shanthi, S.; Vaseeharan, B.

    2015-02-01

    In this study, zinc oxide nanoparticles were biologically synthesized using the leaf extract of Plectranthus amboinicus (Pam-ZnO NPs). The synthesized Pam-ZnO NPs were characterized by UV-Vis spectrophotometer, FTIR, TEM and XRD analysis. TEM analysis of Pam-ZnO NPs showed the average size of about 20-50 nm. Pam-ZnO NPs control the growth of methicillin-resistant Staphylococcus aureus biofilms (MRSA ATCC 33591) at the concentration of 8-10 μg/ml. Confocal laser scanning microscope (CLSM) images revealed that Pam-ZnO NPs strongly inhibited the biofilm forming ability of S. aureus. In addition, Pam-ZnO NPs showed 100% mortality of fourth instar mosquito larvae of Anopheles stephensi, Culex quinquefasciatus and Culex tritaeniorhynchus at the concentration of 8 and 10 μg/ml. The histopathological studies of Pam-ZnO NPs treated A. stephensi and C. quinquefasciatus larvae revealed the presence of damaged cells and tissues in the mid-gut. The damaged tissues suffered major changes including rupture and disintegration of epithelial layer and cellular vacuolization. The present study conclude that Pam-ZnO NPs showed effective control of S. aureus biofilms and mosquito larvae by damaging the mid gut cells.

  7. Teratogenic effect of Euratorium adenophorum spreng extract in rats%紫茎泽兰醇提取物对大鼠致畸作用

    Institute of Scientific and Technical Information of China (English)

    李厚勇; 王蕊; 张振铃; 徐明; 王蓉; 修海迪

    2011-01-01

    目的 探讨紫茎泽兰醇提取物对大鼠致畸作用.方法 按照《农药登记毒理学试验方法》,将孕鼠随机分为5组,紫茎泽兰高、中、低剂量组(分别于孕第6~16d经口灌胃给予1 000、500、200 mg/kg紫茎泽兰醇提取物),阴性对照组(蒸馏水),阳性对照组(阿司匹林300 mg/kg).于妊娠第20d处死孕鼠,观察孕鼠生殖情况和胎鼠生长发育、骨骼内脏畸形情况.结果 紫茎泽兰中、高剂量组吸收胎率分别为15.08%和7.62%,与阴性对照组比较,差异有统计学意义(P<0.05),其它各剂量紫茎泽兰组孕鼠进食、活动、体重增长、死胎率均未见明显影响;与阴性对照组比较,胎鼠体重、身长、尾长无明显差异(P>0.05);胎鼠外观、骨骼、内脏检查均未发现明显畸形.结论 紫茎泽兰醇提取物具有一定胚胎毒性,无明显致畸作用.%Objective To study the teratogenicity of Euratorium adenophorwn spreng extract in rats. Methods According to toxicological test methods of pesticides for registration,the pregnant rats were divided into 5 groups;three Euratorium adenophorwn spreng extract dosage groups(1000,500,200 mg/kg) .negative control group and positive control group(300 mg/kg aspirin). Euratorium adenophorum spreng extract and aspirin were given orally from 6th to 16th day of gestation. The pregnant rats were sacrificed on the 20th day of gestation. Embryonic and fetal developmental parameters were surveyed. The appearance, viscera and skeleton of the fetuses were examined. Results Absorbed fetus rate in moderate and high dose groups was 7. 62% and 15. 08% .showing obvious increases compared with negative control group. There was no difference in the rate of fetal mortality and weight gain in all treated and negative control group. The weight, body length and tail length showed no significant difference between the treated groups and negative control group. Euratorium adenophorum spreng extract didn' t induce obvious

  8. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts.

    Science.gov (United States)

    Logarto Parra, A; Silva Yhebra, R; Guerra Sardiñas, I; Iglesias Buela, L

    2001-09-01

    Artemia salina L. (Artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemical and natural products. In this study the Medium Lethal Concentrations (LC50 value) of 20 plant extracts, Aloe vera (L.) Burm. F. (Aloeaceae), Artemisia absinthium L. (Asteraceae); Citrus aurantium L. (Rutaceae); Cymbopogon citratus (DC. Ex Nees) Stapf (Poaceae); Datura stramonium L. (Solanaceae); Justicia pectoralis Jacq. (Acanthaceae); Musa x paradisiaca L. (Musaceae); Ocimum basilicum L.; O. gratissimum L.; O. tenuiflorum L. (Lamiaceae); Pimenta dioica (L.) Merr. (Myrtaceae); Piper auritum Kunth (Piperaceae); Plantago major L. (Plantaginaceae); Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae); Ruta graveolens L. (Rutaceae); Senna alata (L.) Roxb. (Fabaceae); Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae); and Thuja occidentalis L. (Cupressaceae), were determined using Artemia salina L. (Artemiidae), with the objective of relating the results to the LD50 values reported in mice (tested at three concentrations: 10, 100, and 1000 microg/mL, for each extract). We found good correlation between the in vivo and the in vitro tests (r = 0.85 p < 0.05), and this method is a useful tool for predicting oral acute toxicity in plant extracts.

  9. Screening of medicinal plants for induction of somatic segregation activity in Aspergillus nidulans.

    Science.gov (United States)

    Ramos Ruiz, A; De la Torre, R A; Alonso, N; Villaescusa, A; Betancourt, J; Vizoso, A

    1996-07-01

    Knowledge about mutagenic properties of plants commonly used in traditional medicine is limited. A screening for genotoxic activity was carried out in aqueous or alcoholic extracts prepared from 13 medicinal plants widely used as folk medicine in Cuba: Lepidium virginicum L. (Brassicaceae): Plantago major L. and Plantago lanceolata L. (Plantaginaceae); Ortosiphon aristatus Blume, Mentha x piperita L., Melissa officinalis L. and Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae); Cymbopogon citratus (DC.) Stapf (Poaceae); Passiflora incarnata L. (Passifloraceae); Zingiber officinale Roscoe (Zingiberaceae); Piper auritum HBK. (Piperaceae); Schinus terebinthifolius Raddi (Anacardeaceae) and Momordica charantia L. (Cucurbitaceae). A plate incorporation assay with Aspergillus nidulans was employed, allowing detection of somatic segregation as a result of mitotic crossing-over, chromosome malsegregation or clastogenic effects. Aspergillus nidulans D-30, a well-marked strain carrying four recessive mutations for conidial color in heterozygosity, which permitted the direct visual detection of segregants, was used throughout this study. As a result, only in the aqueous extract of one of the plants screened (Momordica charantia) a statistical significant increase in the frequency of segregant sectors per colony was observed, and consequently, a genotoxic effect is postulated.

  10. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD50 value) in mice, to determine oral acute toxicity of plant extracts.

    Science.gov (United States)

    Logarto Parra, A; Silva Yhebra, R; Guerra Sardiñas, I; Iglesias Buela, L

    2001-09-01

    Artemia salina L. (Artemiidae), the brine shrimp larva, is an invertebrate used in the alternative test to determine toxicity of chemical and natural products. In this study the Medium Lethal Concentrations (LC50 value) of 20 plant extracts, Aloe vera (L.) Burm. F. (Aloeaceae), Artemisia absinthium L. (Asteraceae); Citrus aurantium L. (Rutaceae); Cymbopogon citratus (DC. Ex Nees) Stapf (Poaceae); Datura stramonium L. (Solanaceae); Justicia pectoralis Jacq. (Acanthaceae); Musa x paradisiaca L. (Musaceae); Ocimum basilicum L.; O. gratissimum L.; O. tenuiflorum L. (Lamiaceae); Pimenta dioica (L.) Merr. (Myrtaceae); Piper auritum Kunth (Piperaceae); Plantago major L. (Plantaginaceae); Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae); Ruta graveolens L. (Rutaceae); Senna alata (L.) Roxb. (Fabaceae); Stachytarpheta jamaicensis (L.) Vahl (Verbenaceae); and Thuja occidentalis L. (Cupressaceae), were determined using Artemia salina L. (Artemiidae), with the objective of relating the results to the LD50 values reported in mice (tested at three concentrations: 10, 100, and 1000 microg/mL, for each extract). We found good correlation between the in vivo and the in vitro tests (r = 0.85 p < 0.05), and this method is a useful tool for predicting oral acute toxicity in plant extracts. PMID:11695884

  11. Screening of medicinal plants for induction of somatic segregation activity in Aspergillus nidulans.

    Science.gov (United States)

    Ramos Ruiz, A; De la Torre, R A; Alonso, N; Villaescusa, A; Betancourt, J; Vizoso, A

    1996-07-01

    Knowledge about mutagenic properties of plants commonly used in traditional medicine is limited. A screening for genotoxic activity was carried out in aqueous or alcoholic extracts prepared from 13 medicinal plants widely used as folk medicine in Cuba: Lepidium virginicum L. (Brassicaceae): Plantago major L. and Plantago lanceolata L. (Plantaginaceae); Ortosiphon aristatus Blume, Mentha x piperita L., Melissa officinalis L. and Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae); Cymbopogon citratus (DC.) Stapf (Poaceae); Passiflora incarnata L. (Passifloraceae); Zingiber officinale Roscoe (Zingiberaceae); Piper auritum HBK. (Piperaceae); Schinus terebinthifolius Raddi (Anacardeaceae) and Momordica charantia L. (Cucurbitaceae). A plate incorporation assay with Aspergillus nidulans was employed, allowing detection of somatic segregation as a result of mitotic crossing-over, chromosome malsegregation or clastogenic effects. Aspergillus nidulans D-30, a well-marked strain carrying four recessive mutations for conidial color in heterozygosity, which permitted the direct visual detection of segregants, was used throughout this study. As a result, only in the aqueous extract of one of the plants screened (Momordica charantia) a statistical significant increase in the frequency of segregant sectors per colony was observed, and consequently, a genotoxic effect is postulated. PMID:8771452

  12. Screening of plants acting against Heterometrus laoticus scorpion venom activity on fibroblast cell lysis.

    Science.gov (United States)

    Uawonggul, Nunthawun; Chaveerach, Arunrat; Thammasirirak, Sompong; Arkaravichien, Tarinee; Chuachan, Chattong; Daduang, Sakda

    2006-01-16

    The aqueous extracts of 64 plant species, listed as animal- or insect-bite antidotes in old Thai drug recipes were screened for their activity against fibroblast cell lysis after Heterometrus laoticus scorpion venom treatment. The venom was preincubated with plant extract for 30 min and furthered treated to confluent fibroblast cells for 30 min. More than 40% efficiency (test/control) was obtained from cell treatment with venom preincubated with extracts of Andrographis paniculata Nees (Acanthaceae), Barringtonia acutangula (L.) Gaertn. (Lecythidaceae), Calamus sp. (Palmae), Clinacanthus nutans Lindau (Acanthaceae), Euphorbia neriifolia L. (Euphorbiaceae), Ipomoea aquatica Forssk (Convolvulaceae), Mesua ferrea L. (Guttiferae), Passiflora laurifolia L. (Passifloraceae), Plectranthus amboinicus (Lour.) Spreng. (Labiatae), Ricinus communis L. (Euphorbiaceae), Rumex sp. (Polygonaceae) and Sapindus rarak DC. (Sapindaceae), indicating that they had a tendency to be scorpion venom antidotes. However, only Andrographis paniculata and Barringtonia acutangula extracts provided around 50% viable cells from extract treatments without venom preincubation. These two plant extracts are expected to be scorpion venom antidotes with low cytotoxicity. PMID:16169172

  13. Metabolic adaptations to ammonia-induced oxidative stress in leaves of the submerged macrophyte Vallisneria natans (Lour.) Hara.

    Science.gov (United States)

    Wang, Chao; Zhang, Song He; Wang, Pei Fang; Hou, Jun; Li, Wei; Zhang, Wen Jing

    2008-04-28

    Ammonia (i.e. the total of NH(3) and NH(4)(+)) has been one of the main causes of the decline of macrophytes in fresh water. In order to study the effects of ammonia toxicity, plants of the submersed macrophyte Vallisneria natans (Lour.) Hara were treated with various concentrations of NH(4)Cl (0.1, 0.4, 1.2, 2 and 2.8mM) for 4 days or with 2mM NH(4)Cl for different lengths of time (12h, 1, 2, 4 and 8 days). The toxic effect and oxidative stress caused by NH(4)Cl resulted in a reduction of total chlorophyll (chlorophyll a and b) and an increase in the levels of reactive oxygen species (ROS) O(2)(-) and H(2)O(2), with an increased concentration of NH(4)Cl and duration of exposure. Meanwhile, weak chlorosis and water-soaked symptoms were observed in older leaves exposed to 2.8mM NH(4)Cl for 4 days. The activity of superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR) was up-regulated in leaves treated with 1.2, 2 and 2.8mM NH(4)Cl for 4 days or with 2mM NH(4)Cl for 1, 2 and 4 days, when compared with controls. Among these enzymes, the activity of superoxide dismutase, ascorbate peroxidase and glutathione reductase was significantly up-regulated in plants treated with 0.4mM NH(4)Cl for 4 days, while they were down-regulated at 4 and 8 days from their peak values in leaves treated with 2mM NH(4)Cl. The content of ascorbic acid decreased significantly in leaves treated with 0.4-2.8mM NH(4)Cl for 4 days or with 2mM NH(4)Cl for 2-8 days. The content of total glutathione (tGSH; reduced and oxidized glutathione) increased in leaves treated with NH(4)Cl at 0.4, 1.2 and 2mM for 4 days or with 2mM NH(4)Cl at 1, 2 and 4 days, while tGSH was decreased below the level of controls by treatment with 2.8mM NH(4)Cl for 4 days or to the level of controls by treatment with 2mM NH(4)Cl for 8 days. However, the content of malondialdehyde (MDA) decreased with increased concentration of NH(4)Cl and duration of

  14. The Prevention of Tipburn on Chinese Cabbage (Brassica rapa L. var. pekinensis (Lour. Olson with Foliar Fertilizers and Biostimulators

    Directory of Open Access Journals (Sweden)

    Borkowski Jan

    2016-06-01

    Full Text Available Investigations were carried out in 2008-2010 on Chinese cabbage (Brassica rapa L. var. pekinensis (Lour. Olson. The main problem in cultivation of this vegetable is physiological disorder – tipburn. It is connected with low level of calcium in young leaves and with water deficiency. In 2008, seeds of Chinese cabbage were sown twice, in April and July. In July, the day temperature was high (25-30 °C and relative air humidity was low (35-50%. In these conditions, the young leaves were injured heavily. Rotting was caused by the activity of bacteria Pectobacterium carotovorum subsp. carotovorum (Jones Hauben et al. However, three times foliar application of 1.5% calcium nitrate or 1.5% Wapnovit significantly reduced the tipburn. Also spraying with 0.03% of Tytanit (containing ions of titanium or with 2.5% of Biochikol 020 PC (containing chitosan gave similar effect. In these conditions, application of 1.5% K-300 (containing potassium oxide and ammonium nitrate exacerbated symptoms of tipburn. Application of Wapnovit or Tytanit reduced instantly rotting of heads contrary to the application of their mixture. In autumn cultivation, when the relative air humidity was 80-100%, spraying with 1.5% solution of K-300 significantly decreased injuries in comparison to control. Application of Wapnovit, K-300, Biochikol, Tytanit or the mixture of Biochikol and calcium nitrate eliminated rotting. In experiments done in the springs of 2009 and 2010, when weather conditions were less favorable for tipburn appearance, a severity of it was lower but application of K-300 increased it appearance. In these experiments, Biochikol and Wapnovit eliminated rotting of heads. The results of three years of study have shown that calcium nitrate, Wapnovit, Tytanit and Biochikol limited occurrence of tipburn and bacterial rotting of Chinese cabbage, but the weather conditions during cultivation had the greatest impact on the severity of tipburn.

  15. 细叶美女樱的栽培及应用研究%Studies on the Cultivation and its Application of Verbena tenera spreng

    Institute of Scientific and Technical Information of China (English)

    宋良红

    2003-01-01

    @@ 细叶美女樱(verbena tenera Spreng)马鞭草科,马鞭草属,原产于巴西、秘鲁和乌拉圭等美洲热带地区,我国的北京、上海等地有引种栽培.自1992年春季首次从北京市农林科学院引入郑州市后,我们对其进行了物候观测、栽培试验和应用研究,总结出了细叶美女樱在郑州地区的栽培管理要点,同时大量推广应用,取得了良好的效益.

  16. Tissue Culture and Rapid Propagation of Verbena rigida Spreng%直立美人樱的组织培养与快速繁殖

    Institute of Scientific and Technical Information of China (English)

    杨军方; 李大力; 郑换男; 杨成丽

    2009-01-01

    @@ 1 植物名称直立美人樱(Verbena rigida Spreng),又名美女樱、铺地锦、四季绣球等. 2 材料类别带腋芽的茎段. 3 培养条件(1)芽诱导培养基:MS+6-BA 2.0 mg·L~(-1)(单位下同)+NAA 0.3;(2)增殖培养基:MS+6-BA3.0+NAA 1.0:(3)生根培养基:1/2MS+NAA 0.5.以上培养基均添加3%蔗糖和0.7%琼脂,pH 5.8~6.0.

  17. Wood anatomy of Mollinedia glabra (Spreng. Perkins (Monimiaceae in two Restinga Vegetation Formations at Rio das Ostras, RJ, Brazil

    Directory of Open Access Journals (Sweden)

    Fernanda da S. Novaes

    2010-12-01

    Full Text Available This paper aimed to characterize the anatomical structure of the wood of specimens of Mollinedia glabra (Spreng. Perkins growing in two contiguous formations of restinga vegetation at Praia Virgem, in the municipality of Rio das Ostras, RJ. Both the Open Palmae (OPS and the Sandy Strip Closed Shrub (SSCS formations are found in coastal regions that receive between 1,100 and 1,300 mm of rainfall per year. Sapwood samples were collected in both formations. Typical anatomical features for this species include: solitary vessels, radial multiples or clusters elements, that are circular to angular in outline, 5-15 barred scalarifor perforation plates, ood parenchya scanty, septate fiber-tracheids, and wide multiseriate rays with prismatic crystals. Statistical analyses indicated a significant increase in the frequency of vessel elements and an increase in fiber-tracheid diameters in OPS individuals. These characteristics are considered structural adaptations to increased water needs caused by a greater exposure to sunlight. Continuous pruning may be responsible for the tyloses observed in OPS plants. The greater lengths and higher frequencies of the rays in SSCS trees ay be due to the greater diaeters of their branches. ur results suggest that . glabra develops structural adaptations to the restinga micro-environmental variations during its development.Este trabalho objetiva caracterizar a estrutura anatômica do lenho de Mollinedia glabra, ocorrente em duas formações vegetais contíguas na restinga da Praia Virgem, município de Rio das Ostras, RJ. Essas formações Arbustiva Aberta de Pal-mae (AAP e Arbustiva Fechada do Cordão Arenoso (AFCA estão sobre cordão arenoso e recebem precipitações anuais de 1.100-1.300 mm. Foram obtidas amostras do lenho das duas forações. São características anatôicas gerais da espécie: elementos de vasos solitários, em arranjos radiais ou cachos, de seção de circular a angular; placa de perfuração es

  18. Levantamento do geopatrimónio no concelho de Loures e definição de percursos geoturísticos

    OpenAIRE

    Santos, Rogério Nuno Silva

    2014-01-01

    O presente trabalho, sob a forma de Relatório de Estágio, constitui a fase final do Mestrado de Geografia Física e Ordenamento do Território e teve como objeto de estudo o levantamento do geopatrimónio do Município de Loures e posterior criação de percursos geoturísticos. Para tal, foi indispensável a discussão de alguns conceitos como geodiversidade, geopatrimónio, geossítio, património geomorfológico, geomorfossítio, geoconservação, geoturismo e pedestrianismo. Foram estabelecidas metodo...

  19. Assessment of physical vulnerability of buildings and analysis of landslide risk at the municipal scale: application to the Loures municipality, Portugal

    Science.gov (United States)

    Guillard-Gonçalves, C.; Zêzere, J. L.; Pereira, S.; Garcia, R. A. C.

    2016-02-01

    This study offers a semi-quantitative assessment of the physical vulnerability of buildings to landslides in a Portuguese municipality (Loures), as well as the quantitative landslide risk analysis computed as the product of the landslide hazard by the vulnerability and the economic value of the buildings. The hazard was assessed by combining the spatiotemporal probability and the frequency-magnitude relationship of the landslides. The physical vulnerability assessment was based on an inquiry of a pool of European landslide experts and a sub-pool of landslide experts who know the study area, and the answers' variability was assessed with standard deviation. The average vulnerability of the basic geographic entities was compared by changing the map unit and applying the vulnerability to all the buildings of a test site, the inventory of which was listed on the field. The economic value was calculated using an adaptation of the Portuguese Tax Services approach, and the risk was computed for different landslide magnitudes and different spatiotemporal probabilities. As a rule, the vulnerability values given by the sub-pool of experts who know the study area are higher than those given by the European experts, namely for the high-magnitude landslides. The obtained vulnerabilities vary from 0.2 to 1 as a function of the structural building types and the landslide magnitude, and are maximal for 10 and 20 m landslide depths. However, the highest risk was found for the landslides that are 3 m deep, because these landslides combine a relatively high frequency in the Loures municipality with a substantial potential damage.

  20. Study on the Technology for Extracting Flavonoids in Tamarix chinensis Lour by Macroporous Resin%大孔树脂纯化柽柳总黄酮工艺的研究

    Institute of Scientific and Technical Information of China (English)

    沈广志; 金艳霞; 于春光

    2012-01-01

    Objective To study the conditions for isolating and purifying flavonoids in Tamarix chinensis Lour by D-101 type of niacroporous adsorption resin. Methods The total flavonoids in Tamarix chinensis Lour was taken as a reference index. The sample volume, adsorption time, eluting velocity, ethanol concentration and pH value of the sample solution were investigated. Results The optimum conditions for isolating and purifying flavonoids in Tamarix chinensis Lour by D-101 type of macroporous adsorption resin were as follows; pH = 6-7, 15 mL sample of the original liquid, adsorption time of 30 min, 70% ethanol as an eluting reagent, and an eluting velocity of 3 BV ? H-1. Conclusion D-101 macroporous adsorptive resin can effectively isolate and purify total flavones from Tamarix chinensis Lour.%目的 研究D-101大孔吸附树脂分离纯化柽柳总黄酮的工艺条件.方法 以柽柳总黄酮为指标,对上样量、吸附时间、洗脱速度、乙醇浓度和上样药液pH进行考察.结果 大孔树脂分离柽柳总黄酮的最佳工艺条件为上样量15 mL,pH 6~7,吸附30 min,流速3 BV·h-1,乙醇浓度70%,乙醇用量3 BV.结论 D-101大孔吸附树脂能有效分离纯化柽柳总黄酮.

  1. 宽筋藤化学成分定性鉴别的试验研究%Test Study on the Qualitative Identification of Chemical Components of Tinospora sinensis(Lour.)Merr.

    Institute of Scientific and Technical Information of China (English)

    吴凤荣; 曾聪彦; 胡玉良

    2015-01-01

    目的:考察岭南特色药材宽筋藤所含的化学成分。方法采用试管试验法,对宽筋藤的水、95%乙醇、石油醚提取物进行系统化学实验研究,通过多种显色剂和指示剂的颜色反应或沉淀反应,初步推断宽筋藤中可能含有的化学成分。结果经过系统溶剂预试验研究,提示宽筋藤中可能含有氨基酸、鞣质、有机酸、多糖、香豆素、醌类、强心苷、生物碱、甾体类等化学成分。结论宽筋藤中含有多种生物活性成分,值得进深入研究和开发。%Objective To investigate the chemical components of Tinospora sinensis(Lour. )Merr. , the special herbal material in Lingnan region. Methods The tube test method was adopted for the systematic chemical laboratory research on Tinospora sinensis(Lour. )Merr. with water,95% ethyl alcohol and petrole-um ether extract separately. Through the color reaction or precipitation reaction with a variety of chromogenic agents and indicators,the potential chemical components of Tinospora sinensis(Lour. )Merr. were analyzed. Results After solvent preliminary test study,it was suggested that amino acides,tannins,organic acides,pol-ysaccharides,coumarin,quinones,cardiac glycosides,alkaloids and sterioids were probably contained. Con-clusion Tinospora sinensis(Lour. )Merr. contains many biological active components. A deeper study and exploration are required.

  2. Test Study on the Qualitative Identification of Chemical Components of Tinospora sinensis(Lour.)Merr.%宽筋藤化学成分定性鉴别的试验研究

    Institute of Scientific and Technical Information of China (English)

    吴凤荣; 曾聪彦; 胡玉良

    2015-01-01

    目的:考察岭南特色药材宽筋藤所含的化学成分。方法采用试管试验法,对宽筋藤的水、95%乙醇、石油醚提取物进行系统化学实验研究,通过多种显色剂和指示剂的颜色反应或沉淀反应,初步推断宽筋藤中可能含有的化学成分。结果经过系统溶剂预试验研究,提示宽筋藤中可能含有氨基酸、鞣质、有机酸、多糖、香豆素、醌类、强心苷、生物碱、甾体类等化学成分。结论宽筋藤中含有多种生物活性成分,值得进深入研究和开发。%Objective To investigate the chemical components of Tinospora sinensis(Lour. )Merr. , the special herbal material in Lingnan region. Methods The tube test method was adopted for the systematic chemical laboratory research on Tinospora sinensis(Lour. )Merr. with water,95% ethyl alcohol and petrole-um ether extract separately. Through the color reaction or precipitation reaction with a variety of chromogenic agents and indicators,the potential chemical components of Tinospora sinensis(Lour. )Merr. were analyzed. Results After solvent preliminary test study,it was suggested that amino acides,tannins,organic acides,pol-ysaccharides,coumarin,quinones,cardiac glycosides,alkaloids and sterioids were probably contained. Con-clusion Tinospora sinensis(Lour. )Merr. contains many biological active components. A deeper study and exploration are required.

  3. Atividade antioxidante e toxicidade preliminar do extrato e frações obtidas das folhas e cascas do caule de Dasyphyllum tomentosum (Spreng. Cabrera

    Directory of Open Access Journals (Sweden)

    C.S. Paula

    2014-06-01

    Full Text Available Dasyphyllum tomentosum (Spreng. Cabrera, açucará ou espinho-de-agulha, pertence à família Asteraceae, a qual compreende muitas espécies com propriedades terapêuticas. O objetivo deste trabalho foi avaliar extratos e frações de folhas e de cascas do caule de D. tomentosum, com relação as atividade antioxidante, citotóxica e hemolítica em testes in vitro. Todas as amostras apresentaram atividade antioxidante pelo método de inibição de DPPH, com destaque para a fração acetato de etila obtida das folhas cuja atividade foi comparável à dos padrões ácido ascórbico e rutina. Com relação à redução do complexo fosfomolibdênio, observou-se que esta mesma fração foi semelhante somente a rutina enquanto a fração obtida das cascas do caule apresentou resultado superior. Não foi observada atividade citotóxica e hemolítica frente aos modelos utilizados com os extratos e frações. Os resultados obtidos demonstram o potencial antioxidante da espécie sem apresentar toxicidade.

  4. Semi-quantitative assessment of the physical vulnerability of buildings for the landslide risk analysis. A case study in the Loures municipality, Lisbon district, Portugal

    Science.gov (United States)

    Guillard-Gonçalves, Clémence; Zêzere, José Luis; Pereira, Susana; Garcia, Ricardo

    2016-04-01

    The physical vulnerability of the buildings of Loures (a Portuguese municipality) to landslides was assessed, and the landslide risk was computed as the product of the landslide hazard by the vulnerability and the market economic value of the buildings. First, the hazard was assessed by combining the spatio-temporal probability and the frequency-magnitude relationship of the landslides, which was established by plotting the probability of a landslide area. The susceptibility of deep-seated and shallow landslides was assessed by a bi-variate statistical method and was mapped. The annual and multiannual spatio-temporal probabilities were estimated, providing a landslide hazard model. Then, an assessment of buildings vulnerability to landslides, based on an inquiry of a pool of landslide European experts, was developed and applied to the study area. The inquiry was based on nine magnitude scenarios and four structural building types. A sub-pool of the landslide experts who know the study area was extracted from the pool, and the variability of the answers coming from the pool and the sub-pool was assessed with standard deviation. Moreover, the average vulnerability of the basic geographic entities was compared by changing the map unit and applying the vulnerability to all the buildings of a test site (included in the study area), the inventory of which was listed on the field. Next, the market economic value of the buildings was calculated using an adaptation of the Portuguese Tax Services approach. Finally, the annual and multiannual landslide risk was computed for the nine landslide magnitude scenarios and different spatio-temporal probabilities by multiplying the potential loss (Vulnerability × Economic Value) by the hazard probability. As a rule, the vulnerability values given by the sub-pool of experts who know the study area are higher than those given by the European experts, namely for the high magnitude landslides. The obtained vulnerabilities vary from 0

  5. Impact of Precooling and Controlled-Atmosphere Storage on γ-Aminobutyric Acid (GABA) Accumulation in Longan (Dimocarpus longan Lour.) Fruit.

    Science.gov (United States)

    Zhou, Molin; Ndeurumio, Kessy H; Zhao, Lei; Hu, Zhuoyan

    2016-08-24

    Longan (Dimocarpus longan Lour.) fruit cultivars 'Chuliang' and 'Shixia' were analyzed for γ-aminobutyric acid (GABA) accumulation after precooling and in controlled-atmosphere storage. Fruit were exposed to 5% O2 plus 3%, 5%, or 10% CO2 at 4 °C, and GABA and associated enzymes, aril firmness, and pericarp color were measured. Aril softening and pericarp browning were delayed by 5% CO2 + 5% O2. GABA concentrations and glutamate decarboxylase (GAD; EC 4.1.1.15) activities declined during storage at the higher-CO2 treatments. However, GABA aminotransferase (GABA-T; EC 2.6.1.19) activities in elevated CO2-treated fruit fluctuated during storage. GABA concentrations increased after precooling treatments. GAD activity and GABA-T activity were different between cultivars after precooling. GABA concentrations in fruit increased after 3 days of 10% CO2 + 5% O2 treatment and then declined as storage time increased. GABA accumulation was associated with stimulation of GAD activity rather than inhibition of GABA-T activity.

  6. Impact of Precooling and Controlled-Atmosphere Storage on γ-Aminobutyric Acid (GABA) Accumulation in Longan (Dimocarpus longan Lour.) Fruit.

    Science.gov (United States)

    Zhou, Molin; Ndeurumio, Kessy H; Zhao, Lei; Hu, Zhuoyan

    2016-08-24

    Longan (Dimocarpus longan Lour.) fruit cultivars 'Chuliang' and 'Shixia' were analyzed for γ-aminobutyric acid (GABA) accumulation after precooling and in controlled-atmosphere storage. Fruit were exposed to 5% O2 plus 3%, 5%, or 10% CO2 at 4 °C, and GABA and associated enzymes, aril firmness, and pericarp color were measured. Aril softening and pericarp browning were delayed by 5% CO2 + 5% O2. GABA concentrations and glutamate decarboxylase (GAD; EC 4.1.1.15) activities declined during storage at the higher-CO2 treatments. However, GABA aminotransferase (GABA-T; EC 2.6.1.19) activities in elevated CO2-treated fruit fluctuated during storage. GABA concentrations increased after precooling treatments. GAD activity and GABA-T activity were different between cultivars after precooling. GABA concentrations in fruit increased after 3 days of 10% CO2 + 5% O2 treatment and then declined as storage time increased. GABA accumulation was associated with stimulation of GAD activity rather than inhibition of GABA-T activity. PMID:27412947

  7. CYTOTOXIC ACTIVITY OF N-HEXANE, CHLOROFORM AND CARBON TETRACHLORIDE FRACTIONS OF THE ETHANOLIC EXTRACT OF LEAVES AND STEMS OF BACCAUREA RAMIFLORA (LOUR..

    Directory of Open Access Journals (Sweden)

    Apurba Sarker Apu et al.

    2012-03-01

    Full Text Available The purpose of the study was to find out the cytotoxic activity of the n-hexane, chloroform and carbon tetrachloride fractions of the ethanolic extracts of the leaves and stems of Baccaurea ramiflora (Lour.. Ethanolic extracts of the leaves and stems of Baccaurea ramiflora were subjected to solvent-solvent partitioning using n-hexane, chloroform and carbon tetrachloride to obtain n-hexane leaves fraction, chloroform leaves fraction, n-hexane stems fraction, chloroform stems fraction and carbon tetrachloride stems fraction. Each fraction was assayed for their cytotoxic effect using brine shrimp lethality bioassay. Among the fractions, the n-hexane fractions of leaves and stems showed significant cytotoxic effects having LC50 values of 7.79 µg/ml (95% confidence interval 6.48-9.37 and 5.78 µg/ml (95% confidence interval 4.76-6.99 respectively as compared to vincristine sulfate (LC50= 2.81 µg/ml (95% confidence interval 1.97-4.01 which was used as positive control. The results support the traditional uses of B. ramiflora for various medicinal purposes and thus demand the isolation and identification of active principles and thorough bioassay.

  8. Anti-Inflammatory Potential of Ethanolic Leaf Extract of Eupatorium adenophorum Spreng. Through Alteration in Production of TNF-α, ROS and Expression of Certain Genes

    Directory of Open Access Journals (Sweden)

    Ashim K. Chakravarty

    2011-01-01

    Full Text Available Search for a novel anti-inflammatory agent from a herbal source, such as Eupatorium adenophorum Spreng., a plant from the Eastern Himalayas, is of prime interest in the present investigation. Inflammation causes tissue destruction and development of diseases such as asthma, rheumatoid arthritis, and so forth. The ethanolic leaf extract of E. adenophorum (EEA was administered intravenously and in other cases topically at the site of delayed type hypersensitivity (DTH reaction in mouse foot paw induced with dinitrofluorobenzene. EEA can effectively inhibit DTH reaction and bring back normalcy to the paw much earlier than the controls. Efficacy of EEA on regulatory mechanisms for inflammation has also been considered. Intravenous administration of EEA increased the number of CD4+ T cells in spleen and tumor necrosis factor (TNF-α in serum of DTH mice. Initially it was difficult to reconcile with the anti-inflammatory role of EEA and simultaneous induction of TNF-α, an established pro-inflammatory cytokine. EEA induces higher expression of TNF-α gene and amount of the cytokine in serum. We discussed the other role of TNF-α, its involvement in repairing tissue damage incurred in course of inflammatory reaction. EEA also induces TGF-β encoding a cytokine involved in tissue repair mechanism. EEA inhibits expression of another pro-inflammatory cytokine gene IL-1β and downregulates cycloxygenase 2 (COX2 gene responsible for metabolism of inflammatory mediators like prostaglandins. Furthermore, anti-inflammatory role of EEA is also revealed through its inhibition of hydroxyl radical generation. Notably EEA does not necessarily affect the expression of other inflammation-related genes such as IL-6, IL-10 and IKK. The present study reports and analyzes for the first time the anti-inflammatory property of the leaf extract of E. adenophorum.

  9. Potential of Spectral Reflectance as Postharvest Classification Tool for Flower Development of Calla Lily (Zantedeschia aethiopica (L. Spreng. Potencial de la Reflectancia Espectral como Herramienta para la Clasificación Poscosecha del Desarrollo Floral en Cala (Zantedeschia aethiopica (L. Spreng.

    Directory of Open Access Journals (Sweden)

    Antonio J . Steidle Neto

    2009-12-01

    Full Text Available Unsuitable postharvest management is one of the most serious problems that floriculture has to face. An option for reducing postharvest losses is to use automatic systems for flower sorting and classification, which yield consistent results, reduce costs and speed up these tasks. The objective of this work was show the potential of spectral reflectance to distinguish different postharvest development stages of calla lily flowers, Zantedeschia aethiopica (L. Spreng., aiming the use of this technology within automatic systems for flower classification. The measuring equipment was a spectrometer connected to a portable computer and configured for reflectance data acquisition in the 400 to 1000 nm range. Based on the results, it was verified a differentiation between the spectral reflectance curves of calla lily flowers, with gradual decreases on the measured values according to the increase of the senescence stages. Thus, the spectral reflectance has potential to be used in the development of automatic systems for postharvest classification of calla lily flowers.El manejo poscosecha inadecuado es uno de los problemas más serios que la floricultura tiene que enfrentar. Una opción para reducir las pérdidas poscosecha es emplear sistemas automáticos para ordenar y clasificar las flores, los cuales permiten resultados consistentes, reducen gastos y aceleran estas tareas. El objetivo de este trabajo fue demostrar el potencial de la reflectancia espectral para discriminar las diferentes fases de desarrollo poscosecha de flores de cala (Zantedeschia aethiopica [L.] Spreng., visando el uso de esta tecnología en sistemas automáticos para la clasificación de flores. El equipo de medición fue un espectrómetro conectado a un computador portátil y configurado para la adquisición de datos de reflectancia comprendidos en la región espectral de 400 a 1000 nm. Con base en los resultados, se constató una diferencia entre las curvas de reflectancia

  10. Avaliação do efeito de polietilenoglicóis no perfil de extratos de Mikania glomerata Spreng., Asteraceae, e Passiflora edulis Sims, Passifloraceae Evaluation of PEG effects on the extracts obtaining from Passiflora edulis Sims, Passifloraceae, and Mikania glomerata Spreng., Asteraceae

    Directory of Open Access Journals (Sweden)

    Jorge de Medeiros

    2010-11-01

    Full Text Available Os polietilenoglicóis (PEG são polímeros hidrossolúveis capazes de reduzir a constante dielétrica de solventes como a água, e assim são utilizados como cossolventes para solubilização de diferentes fármacos. Atualmente, o PEG tem sido empregado satisfatoriamente na obtenção de extratos de matérias-primas vegetais por favorecer a extração de substâncias com polaridades semelhantes às extraídas pelos solventes hidroetanólicos. O objetivo deste trabalho foi avaliar o efeito da concentração e da massa molar de PEG (400 e 4000 g/mol sobre a extração de flavonoides totais a partir de Passiflora edulis Sims, Passifloraceae, (maracujá e de cumarina a partir da Mikania glomerata Spreng., Asteraceae, (guaco. Este estudo revelou que o aumento da concentração de PEG promoveu um aumento na capacidade extrativa de flavonoides totais e cumarina a partir da Passiflora edulis e Mikania glomerata respectivamente, comportamento atribuído a alterações da constante dielétrica. Apesar de elevar a capacidade extrativa, os líquidos extratores contendo PEG exigiram elevada relação planta:solvente para alcançar teores extrativos semelhante ao solvente hidroetanólico utilizado, com exceção da extração de flavonoides totais com PEG 4000 g/mol a partir da Passilfora edulis.Polyethylene glycols (PEG are hydrosoluble polymers able to reducing dielectric constant of solvents like water and thus are used as cosolvents to enhance the aqueous solubility of several drugs. Actually, the PEG have been successful applied to obtain extracts of plant raw material once can facilitate the extraction of substances with polarities similar to those extracted by hydroethanol solvents. In this study, the objective was to evaluate the effect of PEG concentration and molecular weight (400 and 4000 g/mol on the extraction of total flavonoids from Passiflora edulis Sims, Passifloraceae, and coumarin from Mikania glomerata Spreng., Asteraceae. The results

  11. Initial Studies on NPK Nutrients Absorption of Melastoma dodecandrum Lour under Artificial Cultivation%地稔人工栽培的氮磷钾养分吸收特性初探

    Institute of Scientific and Technical Information of China (English)

    邱才飞; 彭春瑞; 刘光荣; 钱银飞; 邵彩虹; 谢江; 邓国强

    2012-01-01

    Melastoma dodecandrum Lou is a multi-purpose wild plant. Finding out the needed fertilizer characteristics is a key to success for artificial cultivation. Effect of N, P and K to Melastoma dodecandrum Lour growth and nutrient distribution under artificial cultivation was studied in this paper. The results showed that N, P, K can effectively increase Melastoma dodecandrum Lour's branch umber, leaf number, leaf size, flowering capacity, fruit number and dry mater quantity. N fertilizer is the most effective, followed by K and P fertilizer. The study also founds that coordinated application of 3 elements can promote the absorption of each element, and the growth of Melastoma dodecandrum Lour. The cooperative effect of nutrients was NPK > NK > NP > PK.%地稔为野生多用途植物,摸清地稔的需肥特性是决定地稔人工栽培能否成功的关键,研究了在地稔人工栽培中N、P、K三要素对地稔的生长状况及植株养分分配的影响.结果显示:N、P、K均可以有效增加地稔的分枝数、叶片数、叶面积、开花量、结果数和干物量,且以N肥效果最明显,其次是K和P肥,研究还发现三元素间配合施用具有相互促进吸收的作用,对促进地稔生长的效果更加明显,且养分配合效果大小依次是NPK> NK> NP> PK.

  12. Estudio de la composición química de las semillas y aceites seminales de Ligustrum lucidum Ait y Ligustrum sinense Lour

    Directory of Open Access Journals (Sweden)

    Nolasco, S. M.

    2001-02-01

    Full Text Available The fruits of Ligustrum lucidum Ait and Ligustrum sinense Lour were harvested in Olavarria (Province of Buenos Aires, Argentine. The seeds and extracted oils were analysed for characteristics and composition. The seed contained 8,7-10 and 10,7 % (dry basis, respectively. The physicochemical characteristics of the crude oils were: refractive index 1,4647 and 1,4640 (at 25ºC, iodine value 93,7 and 88,8, saponification index 167 and 154, unsaponifiable matter 11 and 18 %, and free fatty acid content of 4,8 and 9,2 (mg KOH/g, respectively. Gas chromatographic analysis of the oils revealed high levels of oleic acid (57,8 and 51,10%, about 25,1 and 29,6 % of saturated acids, respectively, most of them consisting of palmitic acid, but they have a significant content of acid of more than 18 atoms of carbon. The residual seed meals contained low level of crude protein and of available lysine (2.45, 3.65, g/16g N, respectively. Total and phytic acid phosphorous, calcium, ash, crude fiber, and polysaccharides (non presence of starch contents are reported.Frutos de Ligustrum lucidum Ait y Ligustrum sinense Lour fueron cosechados en Olavarría (provincia de Buenos Aires, Argentina y sus semillas separadas en forma manual. Las semillas se agotaron con n-hexano, obteniendo los aceites crudos con rendimientos del 8,7-10% y 10,7 % (base seca, respectivamente. Los aceites crudos se examinaron en sus características fisicoquímicas (Índice de refracción: 1,4647 y 1,4640 (a 25ºC, Indice de iodo: 93,7 y 88,8, Indice de saponificación: 167 y 154, insaponificable: 11 y 18 %, Indice de acidez: 4,8 y 9,2 (mgKOH/g respectivamente. El análisis por cromatografía gaseosa de los ésteres metílicos reveló alto porcentaje de ácido oleico (57,8 y 51,1%, alrededor de un 25,1 y 29,6 % de ácidos saturados, respectivamente, predominando el ácido palmítico, pero con un significativo contenido de ácidos de más de 18 átomos de carbono. Las harinas residuales de

  13. Biosynthesis, Antibacterial Activity and Anticancer Effects Against Prostate Cancer (PC-3) Cells of Silver Nanoparticles Using Dimocarpus Longan Lour. Peel Extract.

    Science.gov (United States)

    He, Yan; Du, Zhiyun; Ma, Shijing; Cheng, Shupeng; Jiang, Sen; Liu, Yue; Li, Dongli; Huang, Huarong; Zhang, Kun; Zheng, Xi

    2016-12-01

    Metal nanoparticles, particularly silver nanoparticles (AgNPs), are developing more important roles as diagnostic and therapeutic agents for cancers with the improvement of eco-friendly synthesis methods. This study demonstrates the biosynthesis, antibacterial activity, and anticancer effects of silver nanoparticles using Dimocarpus Longan Lour. peel aqueous extract. The AgNPs were characterized by UV-vis absorption spectroscopy, X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and Fourier transform infrared spectroscope (FTIR). The bactericidal properties of the synthesized AgNPs were observed via the agar dilution method and the growth inhibition test. The cytotoxicity effect was explored on human prostate cancer PC-3 cells in vitro by trypan blue assay. The expressions of phosphorylated stat 3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. The longan peel extract acted as a strong reducing and stabilizing agent during the synthesis. Water-soluble AgNPs of size 9-32 nm was gathered with a face-centered cubic structure. The AgNPs had potent bactericidal activities against gram-positive and gram-negative bacteria with a dose-related effect. AgNPs also showed dose-dependent cytotoxicity against PC-3 cells through a decrease of stat 3, bcl-2, and survivin, as well as an increase in caspase-3. These findings confirm the bactericidal properties and explored a potential anticancer application of AgNPs for prostate cancer therapy. Further research should be focused on the comprehensive study of molecular mechanism and in vivo effects on the prostate cancer. PMID:27316741

  14. The anti-tubercular activity of Melia azedarach L. and Lobelia chinensis Lour. and their potential as effective anti-Mycobacterium tuberculosis candidate agents

    Institute of Scientific and Technical Information of China (English)

    Won Hyung Choi; In Ah Lee

    2016-01-01

    Objective: To evaluate the anti-mycobacterial activity of Melia azedarach L. (M. azedarach) and Lobelia chinensis Lour. (L. chinensis) extracts against the growth of Mycobacterium tuberculosis (M. tuberculosis). Methods: The anti-M. tuberculosis activity of M. azedarach and L. chinensis extracts were evaluated using different indicator methods such as resazurin microtiter assay (REMA) and mycobacteria growth indicator tube (MGIT) 960 system assay. The M. tuberculosis was incubated with various concentrations (50–800 mg/mL) of the ex-tracts for 5 days in the REMA, and for 4 weeks in MGIT 960 system assay. Results: M. azedarach and L. chinensis extracts showed their anti-M. tuberculosis ac-tivity by strongly inhibiting the growth of M. tuberculosis in a concentration-dependent manner in the REMA and the MGIT 960 system assay. Particularly, the methanol extract of M. azedarach and n-hexane extract of L. chinensis consistently exhibited their effects by effectively inhibiting the growth of M. tuberculosis in MGIT 960 system for 4 weeks with a single-treatment, indicating higher anti-M. tuberculosis activity than other extracts, and their minimum inhibitory concentrations were measured as 400 mg/mL and 800 mg/mL, respectively. Conclusions: These results demonstrate that M. azedarach and L. chinensis extracts not only have unique anti-M. tuberculosis activity, but also induce the selective anti-M. tuberculosis effects by consistently inhibiting or blocking the growth of M. tuberculosis through a new pharmacological action. Therefore, this study suggests the potential of them as effective candidate agents of next-generation for developing a new anti-tuberculosis drug, as well as the advantage for utilizing traditional medicinal plants as one of effective strategies against tuberculosis.

  15. Salicylic acid involved in the regulation of nutrient elements uptake and oxidative stress in Vallisneria natans (Lour.) Hara under Pb stress.

    Science.gov (United States)

    Wang, Chao; Zhang, Songhe; Wang, Peifang; Hou, Jun; Qian, Jin; Ao, Yanhui; Lu, Jie; Li, Li

    2011-06-01

    In this study, the alterations in nutrient elements content, reactive oxygen species level and antioxidant response were studied in leaves of Vallisneria natans (Lour.) Hara exposed to salicylic acid (SA, 10 or 100 μM), or Pb (50 μM) or their combinations for 4d. No significant alterations in Mn and Ca content were observed but content of Cu, Zn, Fe and P decreased in plants exposed to SA alone. SA application inhibited the uptake of Pb and partially reversed Pb-induced the alterations in Mn, Ca and Fe content in leaves of V. natans exposed to 50 μM Pb. The decreased chlorophyll (a+b) and increased malondialdehyde and O(2-) and H(2)O(2) content were detected in plants exposed to 100 μM SA, 50 μM Pb, 10 μM SA+50 μM Pb or 100 μM SA+50 μM Pb. Application SA partially inhibited Pb-induced the increase of malondialdehyde, O(2-) and H(2)O(2) content. 100 μM SA decreased the activity of NADH oxidase and the content of non-protein thiols, carotenoids and ascorbic acid and increased the content of dehydroascorbate in plants treated with or without Pb. SA alone decreased the ascorbate peroxidase activity and increased the catalase and peroxidase activity, while SA application increased catalase activity but had no significant effect on ascorbate peroxidase and peroxidase activity in V. natans exposed to Pb. The results indicate that SA involves in the regulation of Pb uptake, nutrient balance and oxidative stress. PMID:21377190

  16. Biosynthesis, Antibacterial Activity and Anticancer Effects Against Prostate Cancer (PC-3) Cells of Silver Nanoparticles Using Dimocarpus Longan Lour. Peel Extract

    Science.gov (United States)

    He, Yan; Du, Zhiyun; Ma, Shijing; Cheng, Shupeng; Jiang, Sen; Liu, Yue; Li, Dongli; Huang, Huarong; Zhang, Kun; Zheng, Xi

    2016-06-01

    Metal nanoparticles, particularly silver nanoparticles (AgNPs), are developing more important roles as diagnostic and therapeutic agents for cancers with the improvement of eco-friendly synthesis methods. This study demonstrates the biosynthesis, antibacterial activity, and anticancer effects of silver nanoparticles using Dimocarpus Longan Lour. peel aqueous extract. The AgNPs were characterized by UV-vis absorption spectroscopy, X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and Fourier transform infrared spectroscope (FTIR). The bactericidal properties of the synthesized AgNPs were observed via the agar dilution method and the growth inhibition test. The cytotoxicity effect was explored on human prostate cancer PC-3 cells in vitro by trypan blue assay. The expressions of phosphorylated stat 3, bcl-2, survivin, and caspase-3 were examined by Western blot analysis. The longan peel extract acted as a strong reducing and stabilizing agent during the synthesis. Water-soluble AgNPs of size 9-32 nm was gathered with a face-centered cubic structure. The AgNPs had potent bactericidal activities against gram-positive and gram-negative bacteria with a dose-related effect. AgNPs also showed dose-dependent cytotoxicity against PC-3 cells through a decrease of stat 3, bcl-2, and survivin, as well as an increase in caspase-3. These findings confirm the bactericidal properties and explored a potential anticancer application of AgNPs for prostate cancer therapy. Further research should be focused on the comprehensive study of molecular mechanism and in vivo effects on the prostate cancer.

  17. Molecular cloning, characterization and expression analysis of the gene encoding 1-deoxy-D-xylulose 5-phosphate reductoisomerase from Aquilaria sinensis (Lour.) Gilg

    Indian Academy of Sciences (India)

    Juan Liu; Yanhong Xu; Liang Liang; Jianhe Wei

    2015-06-01

    The major constituents of agarwood oils are sesquiterpenes that are obtained from isoprenoid precursors through the plastidial methylerythritol phosphate (MEP) pathway and the cytosolic mevalonate pathway. In this study, a novel full-length cDNA of 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR), which was the second key enzyme in the plastid MEP pathway of sesquiterpenes biosynthesis was isolated from the stem of Aquilaria sinensis (Lour.) Gilg by the methods of reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) technique for the first time, and named as AsDXR. The full-length cDNA of AsDXR was 1768 bp, containing a 1437 bp open reading frame (ORF) encoding a polypeptide of 478 amino acids with a molecular weight of 51.859 kD and the theoretical isoelectric point of 6.29. Comparative and bioinformatic analysis of the deduced AsDXR protein showed extensive homology with DXRs from other plant species, especially Theobroma cacao and Gossypium barbadense, and contained a conserved transit peptide for plastids, and extended pro-rich region and a highly conserved NADPH-binding motif owned by all plant DXRs. Southern blot analysis indicated that AsDXR belonged to a small gene family. Tissue expression pattern analysis revealed that AsDXR expressed strongly in root and stem, but weakly in leaf. Additionally, AsDXR expression was found to be activated by exogenous elicitor of MeJA (methyl jasmonate). The contents of three sesquiterpenes ($\\alpha$-guaiene, $\\alpha$-humulene and $\\delta$-guaiene) were significantly induced by MeJA. This study enables us to further elucidate the role of AsDXR in the biosynthesis of agarwood sesquiterpenes in A. sinensis at the molecular level.

  18. Effect of the water extract of Macrosolen cochinchinensis (Lour.) Tiegh.leaves on7,12-dimethylbenz [a] antracene induced female mice liver carcinogensis%Effect of the water extract of Macrosolen cochinchinensis (Lour.) Tiegh.leaves on 7,12-dimethylbenz [a] antracene induced female mice liver carcinogensis

    Institute of Scientific and Technical Information of China (English)

    Adam Hermawan; Retno urwanti; Nina Artant; Edy Meiyanto

    2011-01-01

    Liver cancer is the third most common cause of death from cancer worldwide.Recently,natural products were used widely as an alternative therapy for liver cancer.Previous study reported Macrosolen cochnichinensis (Lour.) Tiegh.that grows in the host star fruit inhibited breast cancer cells growth in vitro.This study aims to observe the effect of water extract of M.cochinchinensis leaves (MCE) on Balb/c mice hepatocyte after initiation of 7,12-dimethylbenz [a] anthracene (DMBA) as a liver cancer model inducer.The experiment consisted of four mice groups,corn oil solvent control group,the DMBA dose 20 mg/kgBW p.o.ten times twice a week,DMBA+MCE dose 250 mg/kgBW,and DMBA+MCE 750 mg/kgBW.Extract which was dissolved into 0.5% CMC-Na was administered daily by the oral route 1 week before,during and terminated 1 week after the DMBA induction.At the end of the study,rat livers were collected and stained with Haematoxyllene and Eosin (H&E) method.Administration of MCE could not inhibit hepatic carcinogenesis in DMBA-induced female mice.There was no difference in liver tissue histopathology profile between the extract treatment group and DMBA control group.

  19. Oxidação fenólica, tipo de explante e meios de cultura no estabelecimento in vitro de canafístula (Peltophorum dubium (Spreng. Taub..

    Directory of Open Access Journals (Sweden)

    Josiana Scherer Bassan

    2010-08-01

    Full Text Available A canafístula, Peltophorum dubium (Spreng. Taub. é uma espécie florestal nativa e com ampla dispersão geográfica, desempenhando um papel pioneiro nas áreas abertas, em capoeiras e matas degradadas. Apresenta um rápido crescimento e se adapta facilmente, sendo muito recomendada para reflorestamento homogêneo. A madeira é utilizada em construções civis, indústria de móveis, construção naval e militar. A micropropagação é uma técnica utilizada com bastante sucesso, apresentando, entre outras vantagens, um rápido aumento no número de plantas geneticamente idênticas partindo de plantas selecionadas. Os objetivos do trabalho são avaliar a influência da luz no controle da oxidação fenólica dos explantes de canafístula (Peltophorum dubium (Spreng., determinar o meio nutritivo e o tipo de explante mais adequado para o estabelecimento in vitro de canafístula (Peltophorum dubium (Spreng.. Foram realizados dois experimentos. No primeiro, ápices caulinares foram cultivados em meio base MS a 25 ± 3°C, por 7 dias no escuro e, posteriormente, sob fotoperíodo de 16 horas de luz e intensidade luminosa de 20 μmol.m2.s-1, fornecida por lâmpada fluorescente branca durante 21 dias ou permaneceram na câmara de crescimento com exposição à luz durante todo o experimento. Foi empregado o delineamento inteiramente casualizado com cinco repetições contendo quatro unidades experimentais (UE. A oxidação fenólica foi observada após 21 dias de cultivo. Não ocorreu oxidação fenólica em nenhum dos tratamentos analisados. No segundo experimento, ápices caulinares e segmentos nodais foram cultivados em meio base MS e meio WPM. Os explantes foram mantidos em sala de crescimento com fotoperíodo de 16 horas de luz e intensidade luminosa de 20 μmol.m2.s-1, fornecidas por lâmpada fluorescente branca fria e temperatura de 25 ± 3°C. A UE foi composta por um frasco de vidro de 150 mL contendo 30 mL de meio nutritivo e um explante. O

  20. Avaliação in vivo do efeito hipocolesterolêmico e toxicológico preliminar do extrato bruto hidroalcoólico e decocção da Vitex megapotamica (Spreng Moldenke (V. montevidensis Cham. Evaluation of prospective hypocholesterolemic effect and preliminary toxicology of crude extract and decoction from Vitex megapotamica (Spreng Moldenke (V. montevidensis Cham. in vivo

    Directory of Open Access Journals (Sweden)

    Anna Paula Brandt

    2009-06-01

    Full Text Available As doenças cardiovasculares (DC estão, de modo geral, associadas a elevados níveis séricos de lipídeos, atingindo homens e mulheres, sem distinção de idade. Entre as propriedades atribuídas pela medicina popular à Vitex megapotamica (Spreng Moldenke (V. montevidensis Cham -Tarumã estão a de reduzir os níveis séricos de colesterol e triglicerídeo. O principal objetivo do presente estudo foi avaliar o potencial hipocolesterolêmico e hipolipidêmico da V. megapotamica, bem como fazer um estudo toxicológico preliminar. Para tanto, foi realizada indução hiperlipidêmica usando um modelo que preconiza o emprego de propiltiuracil 1,25 mg/300 g de peso e colesterol 200 mg/kg de peso, aplicados via oral em ratos machos pesando 300 ± 10 g. Foi administrado, por via oral, aos animais hiperlipidêmicos previamente induzidos 300 mg/kg de extrato hidroalcoólico das folhas de V. megapotamica ou 300 mL da decocção da casca da planta. Após o final de cada tratamento, o perfil lipídico foi ensaiado, bem como os níveis de glicose, quando relevante. Nossos resultados confirmaram o efeito hipolipidêmico do extrato hidroalcoólico e da decocção pela redução dos níveis séricos de colesterol e triacilglicerol nas concentrações, via e forma utilizadas. Além disso, foi possível verificar que não houve lesão cardíaca, hepática ou renal pelo extrato e decocção utilizados nas avaliações toxicológicas preliminares ensaiadas.The cardiovascular diseases are, in general, associated with high levels of serum lipids which have high incidence in middle-age men and women. Among other properties characterized by popular medicine, the Vitex megapotamica (Spreng Moldenke (V. montevidensis Cham - Tarumã, decreases the serum cholesterol and triacylglycerol levels. The main proposition of the present study was to evaluate the hypocholesterolemic and hipolipidaemic potential of V. megapotamica and to analyze the preliminary toxicity. It was an

  1. Alelopathic effect of aqueous extracts of leucena (Leucaena leucocephala Wit leave and fruit on germination and root growth of canafistula (Peltophorum dubium Spreng/ Efeito alelopático do extrato aquoso de folha e de fruto de leucena (Leucaena leucocephala Wit sobre a germinação e crescimento de raiz da canafístula (Peltophorum dubium Spreng.

    Directory of Open Access Journals (Sweden)

    Andréa Maria Teixeira Fortes

    2005-06-01

    Full Text Available Nowadays exotic plants represent a big threat to biodiversity because when they are present in favorable environments, they can influence the development of native plants, as well as they can change the natural functions of the environment. Wrong management practices are some of the main factors that support the environment invasions by exotic species. Leucena (Leucaena leucocephala Wit. is an exotic species that takes part in Itaipu Dam recuperation riparian areas. Exotic species may have allelopathic effects. The objective of this study has been to verify allelopathic effects of aqueous extracts of leucena leaves and fruits upon germination and root growth of canafistula (Peltophorum dubium Spreng., a native species. Twelve treatments with four replications have been used, divided in two stages, six treatments each. The utilized treatments were: water, 20%, 40%, 60%, 80% and 100% extract. All the seeds were scared mechanically. The germinated seeds have been counted daily. The length of the roots has been evaluated at the third and seventh day of experiment. The fruit extract presented more allelopathic effects than the leaves extract. The roots were sensitive both to the fruits and leaves extract.Atualmente as plantas exóticas representam uma grande ameaça à biodiversidade pois, quando presente em ambientes que lhe são favoráveis, influenciam no desenvolvimento das plantas nativas, além de alterar o funcionamento natural daquele ambiente. Práticas de manejo erradas são um dos principais fatores que favorecem a invasão do ambiente pelas espécies exóticas. A leucena (Leucaena leucocephala Wit. é uma espécie exótica que foi introduzida na recuperação da mata ciliar da Hidrelétrica de Itaipu, e pode possuir efeitos alelopáticos. Objetivou-se neste trabalho verificar a presença de efeitos alelopáticos do extrato aquoso de folha e fruto da leucena sobre a germinação e crescimento de raiz da canafístula (Peltophorum dubium

  2. "Phloem sap analysis of Schleichera oleosa (Lour) Oken, Butea monosperma (Lam) Taub. and Ziziphus mauritiana (Lam) and hemolymph of Kerria lacca (Kerr) using HPLC and tandem mass spectrometry".

    Science.gov (United States)

    Vashishtha, Amit; Rathi, Brijesh; Kaushik, Sandeep; Sharma, K K; Lakhanpaul, Suman

    2013-10-01

    Females of lac insects especially of Kerria lacca (Kerr) secret a resin known as lac for their own protection, which has tremendous applications. Lac insect completes its lifecycle on several host taxa where it exclusively feeds on phloem sap but Schleichera oleosa (Lour.) Oken, Butea monosperma (Lam.) and Ziziphus mauritiana (Lam.) are its major hosts. Analysis of phloem sap constituents as well as hemolymph of lac insect is important because it ultimately gets converted into lac by insect intervention. Main phloem sap constituent's viz. sugars and free amino acids and hemolymph of lac insect were analyzed using HPLC and tandem mass spectrometry, respectively. The results were transformed to relative percentage of the total sugars and free amino acids analyzed in each sample for comparison among lac insect hemolymph and the phloem sap of the three different host taxa. Sucrose (58.9 ± 3.6-85.6 ± 0.9) and trehalose (62.3 ± 0.4) were the predominant sugars in phloem sap of three taxa and hemolymph of lac insect, respectively. Glutamic acid (33.1 ± 1.4-39.8 ± 1.4) was found to be main amino acid among the phloem sap of three taxa while tyrosine (61 ± 2.6) was the major amino acid in hemolymph of lac insect. The relative percentage of non-essential amino acids (60.8 %-69.9 %) was found to be more in all the three host taxa while essential amino acids (30.1 %-35.4 %) were present at a lower relative percentage. In contrast to this, the relative percentage of essential amino acids (81.9 %) was observed to be higher as compared to non-essential amino acids (17.7 %) in lac insect hemolymph. These results led to the detection of lac insect's endosymbionts. Moreover, this study revealed a clue regarding the importance of development of a synthetic diet for this insect so that a precise pathway of lac biosynthesis could be investigated for thorough understanding.

  3. 不同地理种源紫茎泽兰的生态适应性比较%Ecological adaptability of different provenance Eupatorium adenophorum Spreng

    Institute of Scientific and Technical Information of China (English)

    周蒙; 刘文耀; 马文章; 赵相健

    2009-01-01

    采用交互移植法,对移栽在6种不同生境中的5个不同种源紫茎泽兰幼苗的存活率、株高、分枝数、生物量、单株花序数、产种量和种子萌发率进行了为期1年的对比研究.结果表明:各种源紫茎泽兰的幼苗生长和繁殖特性对样地环境条件变化均表现出很强的可塑性.试验样地因素对幼苗株高、分枝数、生物量、单株花序数和产种量的影响均达到极显著水平(P<0.001).随着样地纬度和海拔的升高,各种源的幼苗株高、分枝数量、单株生物量、每株花序数量和单株产种量均呈下降趋势,且各样地间的差异均达到显著水平(P<0.05).但种源因素对幼苗株高、分枝数、生物量、单株花序数和产种量的影响均不显著(P>0.05).除单株产种量外,种源与试验样地的交互作用对上述各指标的影响均不显著.在各样地内,当地种源幼苗的存活率、生长能力和繁殖能力均未表现出显著的优势.说明紫茎泽兰在我国西南地区入侵成功主要依靠其较高的表型可塑性,而局域适应的作用相对较小.%By using reciprocal transplant method, Eupatorium adenophorum Spreng. seedlings of five different provenances were reciprocally transplanted into six different sites in Yunnan Province, with the survival rate, plant height, branch number, biomass, flower number per plant, and seed production measured during the period from April 2007 to May 2008. The seedlings growth and reproductive traits of different provenance E. adenophorum all showed strong plasticity to environmental conditions. With increasing latitude and altitude of transplant site, plant height, branch number, biomass, flower number per plant, and seed production of different provenance E. adenophorum decreased, and the differences in these growth and reproductive traits were significant among the transplant sites. However, there were no significant differences in these traits among different

  4. The ecological and biological characteristics and sowing propagation of Ochna integerrima(Lour.) Merr%金莲木(Ochna integerrima(Lour.)Merr.)的生态生物学特征及其播种繁殖技术

    Institute of Scientific and Technical Information of China (English)

    袁莲莲; 王少平; 任海; 刘楠; 范桑桑; 张佩霞

    2013-01-01

    金莲木(Ochna integerrima (Lour.) Merr.)是金莲木科落叶灌木或小乔木,是潜在的优良园林绿化树种。文章从其形态解剖特征、光合生理生态特征及播种繁殖技术方面进行了系统的研究。结果表明:金莲木为中性偏阳性树种,光合速率日进程呈单峰曲线,无午休现象;其光饱和点(LSP)和光补偿点(LCP)分别在800μmol·m-2·s-1及52.2μmol·m-2·s -1左右。在高于光饱和点的光强下,其叶片的光合速率并未出现明显下降趋势,同时还维持了较高的蒸腾速率。金莲木叶绿素荧光参数显示其具有较高的能量利用效率,叶绿素a/b比值(5.677꞉1)略高于理论值(3꞉1),表明它能有效吸收光能并传到光反应中心。播种前金莲木种子宜进行浸泡处理,浸泡24 h处理后其发芽率高且发芽较早,播种在泥炭土中的种子较沙土中的种子发芽要早且萌发周期短,但在沙土中播种的种子最终的发芽率要相对较高。因此,金莲木适合生长在水热充沛、土壤相对肥沃的热带亚热带地区,栽培时要尽量选择土层肥沃的壤土或砂质壤土。%Ochna integerrima (Lour.) Merr. is a deciduous shrub or small tree species of Ochnaceae family with potentials to be used for landscape greening. We examined the species’ ecological, biological and physiological characteristics and sowing propagation, and found that it was a shade intolerant mesophyte species. Its diurnal net photosynthetic rate in leaves showed a single peaked curve without midday depression. Its light saturation point was 800 μmol·m-2·s-1 and light compensation point was 52.2 μmol·m-2·s-1. When the light intensity was higher than light saturation point, the photosynthetic rate did not decline significantly and kept high transpiration rate. The chlorophyll fluorescence parameters showed that this species had a relatively high level of energy use efficiency. Its chlorophyll a/b ratio(5

  5. Utilização de esgotos domésticos tratados através de um sistema biológico na produção de mudas de Croton floribundus Spreng. (Capixingui E Copaifera langsdorffii Desf. (Copaíba Use of domestic wastewater after biological treatment system for Croton floribundus Spreng. and Copaifera langsdorffii Desf. seedling production

    Directory of Open Access Journals (Sweden)

    Danielle Camargo Celentano Augusto

    2003-06-01

    Full Text Available O presente trabalho teve por objetivo o aproveitamento da água residuária proveniente de um sistema biológico de tratamento de esgotos domésticos como alternativa à fertirrigação convencional de viveiros florestais, visando a produção de mudas de Croton floribundus (capixingui e Copaifera langsdorffii (Copaíba via subirrigação. As variáveis avaliadas mensalmente foram altura e diâmetro do coleto da muda e, ao final do experimento, peso da matéria seca da parte aérea e da raiz, área foliar, razão parte aérea/ raiz, análise nutricional foliar, análises químicas do substrato antes e após o ensaio e análises nutricionais periódicas da água residuária. O crescimento das plantas, em geral, foi superior no tratamento convencional com adubos minerais. O desenvolvimento do sistema radicular foi favorecido no tratamento com água residuária, o que pode ser característica desejável para maior sobrevivência das mudas no campo. Os resultados mostraram que a água residuária pode ser utilizada na fertirrigação de viveiros para produção das espécies estudadas, pois todas as plantas se mostraram vigorosas, com bom desenvolvimento, sem mortalidade, deficiência ou toxidez. Entretanto, constatou-se que essas mudas necessitarão de um maior tempo no viveiro, quando comparadas com as produzidas com fertilizantes minerais.This work aimed to study the use of treated domestic wastewater from a biological treatment system as an alternative for a seedling-nursery conventional fertirrigation (irrigation fertilization of Croton floribundus Spreng. (Euphorbiaceae and Copaifera langsdorffii Desf. (Leguminosae Caesalpinioideae production. The variables analyzed monthly were seedling height and diameter and shoot and root dry matter weight, leaf area, shoot/root ratio, leaf and substrate chemical status (before and after the experiment and periodical waste water nutritional analyses. Plant growth was greater when chemical fertilizers were

  6. Crescimento de plântulas de Pfaffia glomerata (Spreng. Pedersen cultivadas in vitro sob dois níveis de nitrogênio e sacarose, durante seis subculturas sucessivas e aclimatização Growth of Pfaffia glomerata (Spreng. Pedersen in vitro cultured plantlets on two levels of nitrogen and sucrose, during six successive subcultures and acclimatization

    Directory of Open Access Journals (Sweden)

    Joseila Maldaner

    2007-02-01

    Full Text Available O presente trabalho objetivou avaliar o crescimento de ginseng brasileiro (Pfaffia glomerata (Spreng. Pedersen cultivado in vitro sob dois níveis de nitrogênio e sacarose, durante seis subculturas sucessivas e aclimatização. Como fonte de explantes, utilizou-se segmentos nodais de plantas previamente estabelecidas in vitro. Foram testados dois tratamentos: 100% da concentração padrão de N do meio MS + 30g L-1 sacarose; e 50% da concentração padrão de N do meio MS + 45g L-1 sacarose. Durante as seis subculturas sucessivas, os explantes de plantas cultivadas por 30 dias em ambos os tratamentos foram transferidos, respectivamente, para a subcultura seguinte nos mesmos tratamentos. Ao final da 6ª subcultura, as plântulas foram transferidas para substrato Plantmax® Hortaliças e submetidas à aclimatização em sala de crescimento. Durante as subculturas, observou-se flutuações nas respostas de crescimento das plântulas às duas condições nutricionais testadas. A altura da maior brotação, a média da altura das brotações e o total de segmentos nodais por plântula, respectivamente em quatro, quatro e três subculturas, foram maiores em 100% da concentração padrão de N do meio MS + 30g L-1 sacarose do que em 50% da concentração padrão de N do meio MS + 45g L-1 sacarose. Por outro lado, as matérias secas de raízes, da parte aérea e do total das plântulas, respectivamente em duas, duas e três subculturas, foram maiores em 100% da concentração padrão de N do meio MS + 30g L-1 sacarose. Concluiu-se que o aumento da concentração de sacarose associado à redução da concentração do N estimula a produção de biomassa, enquanto o meio MS padrão estimula o crescimento em altura e número de segmentos nodais. As diferenças quanto à produção de biomassa observadas no cultivo in vitro não se refletiram no processo de aclimatização ex vitro, onde o meio MS padrão proporcionou maior crescimento em altura e n

  7. Toxicological screening of Mikania glomerata Spreng., Asteraceae, extract in male Wistar rats reproductive system, sperm production and testosterone level after chronic treatment Triagem toxocológica do extrato de Mikania glomerata Spreng., Asteraceae, no sistema reprodutor, produção espermática e nível de testosterona em machos de ratos Wistar após tratamento crônico

    Directory of Open Access Journals (Sweden)

    Rita de Cássia da Silveira e Sá

    2010-11-01

    Full Text Available Some compounds present in therapeutic plants may be responsible for the occurrence of adverse side effects. Coumarin and flavonoids are substances found in many plant species that showed antifertility activity in female rats and dogs, respectively. Mikania glomerata Spreng., Asteraceae, known as guaco in Brazil, is a plant largely used in folk medicine and its leaves are reported to have coumarin and flavonoids. This work analyzes the effect of chronic administration of M. glomerata on the reproductive system of male rats. Thirty-day-old Wistar rats were treated with M. glomerata hydroalcoholic extract at a dose of 3.3 g/kg of body weight for ninety days. Body and organ weights, gamete concentration on the epididymis cauda, serum testosterone level and food consumption were evaluated. No significant alteration was observed in any of the variables analyzed, suggesting the absence of toxic action or antifertility activity of the M. glomerata hydroalcoholic extract.As plantas medicinais podem apresentar na sua constituição compostos capazes de causar efeitos adversos no organismo. Cumarina e flavonoides são substâncias encontradas em muitas espécies vegetais, cuja interferência na fertilidade de ratas e cadelas, respectivamente, foi evidenciada em estudos prévios. Mikania glomerata Spreng., Asteraceae, (guaco é uma planta usada no tratamento de doenças respiratórias e em suas folhas foi detectada a presença de cumarina e flavonoides. Neste estudo, avaliou-se o efeito do extrato hidroalcoólico, preparado com partes aéreas de guaco, no sistema reprodutor de ratos submetidos a tratamento crônico. Ratos Wistar (trinta dias de idade foram tratados com extrato hidroalcoólico de guaco na dose de 3,3 g/kg de peso corporal durante noventa dias. O peso corporal e de órgãos, a produção de espermatozoides, a concentração de testosterona plasmática e o consumo de ração foram avaliados. Não foi observada nenhuma alteração significativa

  8. Pengaruh Pemberian Ekstrak Herbal Terhadap Produktivitas Dan Mutu Ayam Pedaging

    OpenAIRE

    Supomo

    2016-01-01

    Productivity and quality of broiler are serious problem for breeders. Many studies were performed to solve these problems, such as utilization of natural products. Several plants have ability to increase productivity and quality of broiler. Objective of this study is to investigate effects of the ethanol extracts of Andrographis paniculata (Burm.f.), Cucurma xanthorrhiza Roxb, Plectranhus amboinicus Lour dan Carica papaya L. on productivity and quality of broiler. The subject of research u...

  9. Effects of irradiation-degradated chitosan coating on quality and shelf-life of the fruits of Shatang Mandarin, Fortunella Margariat (LOUR) swingle and Lycopersicon Esculentum MILL. var. cerasiforme alef

    International Nuclear Information System (INIS)

    Effects of irradiation-degradated chiotosan of different molecular weight on weight loss, rotting rate, total acid, soluble solid content, and ascorbic acid in Shatang mandarin, Fortunella margarita (lour) Swingle, and Lycopersicon esculentum Mill. var. cerasiforme Alef during storage were investigated. The result showed that, compared to control, all chitosan treatment could significantly reduce weight loss and rotting rate, and maintain the content of total acid, soluble solid, and ascorbic acid. After 18 days of storage, treatment of chitosan with molecular weight of 6.6 x 104 Da showed the highest capability of decreasing the rotting rate in S. mandarin, F. margarita, L. esculentum by 71.11%, 66.01% and 70.22%, respectively; increasing total acid by 55.60%, 36.75% and 36.68%, soluble solid content by 49.06%, 25.75% and 49.46%, and ascorbic acid by 42.80%, 41.65% and 51.70%, respectively. Accordingly, irradiation-degradated chitosan coating could effectively prevent the rotting rate and preserve the quality of the three kinds of fruit during storage and thus prolong their shelf life to 18 days. (authors)

  10. The Determination of Arbutin in Paederia Scandens (Lour) Merr by Capillary Electrophoresis with Amperometric Detection%毛细管电泳安培法测定鸡矢藤中熊果苷的含量

    Institute of Scientific and Technical Information of China (English)

    陈缵光; 张孔; 莫金垣; 潘爱华; 周琼

    2002-01-01

    @@ 1 引言 鸡矢藤(Herba Paederiae)为茜草科鸡矢藤属植物鸡矢藤(Paederia Scandens (Lour) Merr)的全草或根,为较重要和较常用的草药.药用主要为叶和茎.熊果苷(Arbutin)是鸡矢藤的重要有效成分之一,在药理上能抑制胰岛素的降解,口服后在体内水解产生氢醌而具有杀菌作用.中草药中熊果苷的测定主要有纸上层析-光度法,薄层-光密度法和高效液相色谱法等.毛细管电泳法测定鸡矢藤及熊果苷在国内外还未见报道.本文用毛细管电泳安培法研究了鸡矢藤中熊果苷的测定条件,为中草药开发应用、质量监控和药理研究提供一种快速、灵敏、准确而低成本的分析手段.

  11. Koenimbin, a natural dietary compound of Murraya koenigii (L Spreng: inhibition of MCF7 breast cancer cells and targeting of derived MCF7 breast cancer stem cells (CD44+/CD24-/low: an in vitro study

    Directory of Open Access Journals (Sweden)

    Ahmadipour F

    2015-02-01

    Full Text Available Fatemeh Ahmadipour,1 Mohamed Ibrahim Noordin,1 Syam Mohan,2 Aditya Arya,1 Mohammadjavad Paydar,3 Chung Yeng Looi,3 Yeap Swee Keong,4 Ebrahimi Nigjeh Siyamak,4 Somayeh Fani,1 Maryam Firoozi,5 Chung Lip Yong,1 Mohamed Aspollah Sukari,6 Behnam Kamalidehghan1 1Department of Pharmacy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; 2Medical Research Center, Jazan University, Jazan, Kingdom of Saudi Arabia; 3Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; 4UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Malaysia; 5Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran; 6Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, Serdang, Malaysia Background: Inhibition of breast cancer stem cells has been shown to be an effective therapeutic strategy for cancer prevention. The aims of this work were to evaluate the efficacy of koenimbin, isolated from Murraya koenigii (L Spreng, in the inhibition of MCF7 breast cancer cells and to target MCF7 breast cancer stem cells through apoptosis in vitro. Methods: Koenimbin-induced cell viability was evaluated using the MTT (3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide assay. Nuclear condensation, cell permeability, mitochondrial membrane potential, and cytochrome c release were observed using high-content screening. Cell cycle arrest was examined using flow cytometry, while human apoptosis proteome profiler assays were used to investigate the mechanism of apoptosis. Protein expression levels of Bax, Bcl2, and heat shock protein 70 were confirmed using Western blotting. Caspase-7, caspase-8, and caspase-9 levels were measured, and nuclear factor kappa B (NF-κB activity was assessed using a high-content screening assay. Aldefluor™ and mammosphere formation assays were used to evaluate the effect of koenimbin on MCF7

  12. 白木香内生真菌的分离鉴定及其抑菌活性%Isolation, Identification and Antimicrobial Activity of Endophytic Fungi in Aquilaria sinensis (Lour.) Gilg

    Institute of Scientific and Technical Information of China (English)

    张秀环; 梅文莉; 陈苹; 邓媛元; 戴好富

    2009-01-01

    In the present study, 42 strains of endophytic fungi were isolated from the resin formation section and healthy xylem of Aquilaria sinensis (Lour.) Gilg. Among them, 33 strains with sporulation were morphologically classified into 7 genera, 4 families and 3 orders, and the rest 9 strains were tentatively identified as Mycelia sterilia. The antimicrobial activities of the isolates to 3 bacterial pathogens and 2 tumor cells were tested by cylinder plate method and MTT. The results indicated that the endophytic fungi in the resin formation section had more species than those in the healthy xylem, and Acremonium was the dominant genus in the endophytic fungi from the healthy xylem, while Penicillium was the dominant genus in the endophytic fungi from the resin formation section. 26 strains of the isolates (61.9%) could exhibit antimicrobial activities to at least one of the tested pathogens. 7 strains of the isolates (16.7%) showed cytotoxic activities to the tested tumor cells. Antimicrobial strains mainly distributed in the genera of Penicillium and Acremonium. The strains belonging to Acremonium represented stronger inhibitory activities and their antibacterial components are worthy for further study.%从白木香Aquilaria sinensis (Lour.) Gilg木质部的树脂形成部位和健康部位中共分离获得 42 株内生真菌,经初步鉴定,产孢的 33 株分属于 3 目4科7属,其余未产孢的 9 株暂归为无孢菌群.采用杯碟法和MTT法分别测定了各菌株的发酵上清液对 3 种病原菌的体外抑菌活性和 2 种肿瘤细胞的体外细胞毒活性.结果表明,白木香木质部健康部位内生真菌以枝顶孢霉属为优势属,而树脂形成部位的内生真菌种类比健康部位要多,且以青霉属为优势属.其中 26 株至少能抑制一种指示菌,占总数的61.9%;7 株对指示瘤株具有细胞毒活性,占总数的16.7%.抑菌活性菌株主要分布在枝顶孢霉属和青霉属.枝顶孢霉属菌株抑菌活性较强

  13. 孝顺竹肉桂醇脱氢酶基因的克隆及表达研究%A Study on the Cloning and Expression of a Cinnamoyl Alcohol Dehydrogenas Gene in Bambusa multiplex (Lour.)Raeuschel

    Institute of Scientific and Technical Information of China (English)

    卞林玲; 范军; 程备久; 项艳; 高健

    2011-01-01

    肉桂醇脱氢酶(cinnamoyl alcohol dehydrogenas,CAD)是木质素生物合成过程中的一类关键酶.采用RT-PCR及RACE方法从孝顺竹笋中分离出CAD基因家族的一个基因,cDNA全长是1131 bp(GenBank注册号为GU985522),含有一个1 107 bp的读码框,编码一个368 aa的蛋白,分子量约为39 kD.序列分析结果表明,其编码的氨基酸含有CAD类基因所具有的醇脱氢酶GroES-like结构域和锌结合脱氧酶结构域,与禾本科植物水稻、玉米等有很高的相似性,与水稻相似性高达97.0%.系统进化树分析同样表明,克隆的孝顺竹CAD基因与水稻的CAD基因的亲缘关系最为接近.组织特异性表达分析显示,CAD基因在孝顺竹笋中表达量最高,在茎、叶和杆箨中亦有低水平表达.本研究将为更深入的研究孝顺竹CAD基因的分子调控研究奠定基础.%Cinnamoyl Alcohol Dehydrogenas is a key enzyme in the biosynthesis of lignin. A gene of CAD gene family was cloned by RT-PCR and SMART RACE RT-PCR from the shoots of Bambusa multiplex (Lour. ) Raeuschel ( GenBank accession number: GU985522). The cloned CAD gene of Bambusa multiplex ( Lour. ) Raeuschel, with the cDNA length of 1 131 bp, contained an open reading frame of 1 107 bp which encoded a polypeptide of 368 amino acids with predicted molecular mass of 39 kD. The results of sequence analysis showed that the deduced polypeptide contained alcohol dehydrogenase GroES-like domain and zinc-binding dehydrogenase in the conserved sequences of the CAD family. It was highly homologous to the CAD proteins from O. sativa and Zea mays and its identity to CAD proteins from O. sativa was up to 97.0 %. The phylogenetic tree analysis also indicated that cloned CAD gene has most close relationship with the CAD gene in O. sativa. Tissue specific expression showed that CAD expressed in leaf, sheath, young stem and bamboo shoots, much higher in bamboo shoots. All the study will provide a theoretical basis for further studying

  14. Influências da variação sazonal e tipos de secagem nas características da droga vegetal - raízes de Pfaffia glomerata (Spreng. Pedersen (Amaranthaceae

    Directory of Open Access Journals (Sweden)

    C.L.S. Vigo

    2004-01-01

    Full Text Available Analisou-se a variação sazonal e a influência de diferentes tipos de secagem (sol, sombra e estufa no teor de saponinas e nas características físico-químicas da droga vegetal - raízes de Pfaffia glomerata (Spreng. Pedersen (Amaranthaceae. A avaliação sazonal apontou a ocorrência de diferenças significativas nos teores de extrativos (maior no inverno: 61,1%±1,9, saponinas totais (maior no inverno: 17,1±0,2%, e entre cinzas totais (maiores no inverno e primavera: 6,5±0,4 e 6,5±1,0%, respectivamente e cinzas insolúveis em ácido(maiores no verão e outono: 0,57±0,11 e 0,44±0,18%, repectivamente. Quanto à variação sazonal do teor de saponinas, o valor do lote colhido no inverno foi maior do que nas outras estações, confirmando o conceito geral de maior ocorrência de ativos em órgãos subterrâneos no inverno. Quanto à influência dos métodos de secagem, o lote seco ao sol forneceu maior teor de extrativos (61,2±2,3% do que os lotes secos na sombra e em estufa, contradizendo a regra geral de que a radiação solar degrada os ativos vegetais. Esse mesmo perfil de dados ocorreu com relação ao teor de saponinas, pois o lote seco ao sol mostrou maior valor(15,4% do que daqueles secos na sombra ou em estufa (14,4 e 11,6%, respectivamente. Desta forma, verificou-se que, de fato, ocorre variação sazonal nas raízes de P. glomerata, particularmente, no que diz respeito aos teoes de extrativos, cinzas e saponinas totais, sendo que essas variações indicam maior concentração de ativos nos períodos de inverno e primavera. Dentre os métodos de secagem testados, o emprego do sol parece favorecer os teores de saponinas e de extrativos, embora tal conclusão mereça ser melhor investigada.

  15. 印度传统药用植物可因氏越橘的研究综述%An update on Murraya koenigii Spreng:a multifunctional Ayurvedic herb

    Institute of Scientific and Technical Information of China (English)

    Priyanka Gupta; Alok Nahata; Vinod K. Dixit

    2011-01-01

    可因氏越橘又名调料九里香(Murra ya koenigii,芸香科,九里香属),是一种常用的药用植物,在印度阿育吠陀医学中有数百年的使用历史.这种植物的叶子、果实、根及树皮均富含咔唑生物碱.已有的很多研究报道这些生物碱具有多种药理活性如抗肿瘤、抗病毒、抗炎、止泻、利尿、抗氧化等.除了这些药理活性,该植物还具有多种多样的生物活性.可因氏越橘作为一种能够治疗多种疾病的有价值的药用植物,有关其植物化学及药理学的研究数量众多,因此有必要对其作一系统的综述.本文对有关可因氏越橘的植物化学、药理学、临床及基础研究进行了系统的整理,以期更全面地发掘其作为药用植物的价值.%Murraya koenigii Spreng (Rutaceae),a medicinally important herb of Indian origin,has been used for centuries in the Ayurvedic system of medicine.Leaves,fruits,roots and bark of this plant are a rich source of carbazole alkaloids.These alkaloids have been reported for their various pharmacological activities such as antitumor,antiviral,anti-inflammatory,antidiarrhoeal,diuretic and antioxidant activities.Apart from these activities,the plant is reported to possess a wide spectrum of biological activities.Phytochemistry and pharmacology of this plant make a demand of an exhaustive review of its potential as a valuable therapeutic agent for the treatment and management of various ailments frequently affecting humans.The present review gives a detailed description of the phytochemical,pharmacological,clinical and pre-clinical works carried out on this medicinal herb and also throws light on its therapeutic potential.

  16. ФАРМАКОГНОСТИЧЕСКОЕ ИЗУЧЕНИЕ EUPHRASIA TATARICA FISCH. EX SPRENG. (SCROPHULARIACEAE)

    OpenAIRE

    Бомбела, Татьяна; Кроткова, Ольга; Петриченко, Василий; Белоногова, Валентина

    2012-01-01

    Проведено фармакогностическое изучение Euphrasia tatarica Fisch. ex Spreng. очанки татарской и обоснована возможность её использования в качестве производящего растения для включения в проект ФС «Трава Очанки». Определены макрои микроскопические диагностические признаки, установлены числовые показатели для включения в нормативную документацию. Для количественного определения флавоноидов в траве E. tatarica предложен метод дифференциальной спектрофотометрии, в пересчете на цинарозид....

  17. 遮光度对白木香种子萌发和幼苗生长的影响%Study on the Effects of Shading on Seed Germination and Seedling Growth of Aquilaria sinensis (Lour.) Glg

    Institute of Scientific and Technical Information of China (English)

    袁莲珍; 史富强; 侯云萍; 徐玉梅

    2015-01-01

    采用遮光网探讨不同遮光度对白木香种子萌发和幼苗生长的影响。结果表明:遮光度75%、50%条件下,白木香种子萌发时间最早,萌发率达到80%以上;自然光( CK)条件下,萌发率最低,萌发时间最迟。不同遮光度对白木香幼苗生长有极显著影响,遮光度75%、50%条件下,白木香幼苗存活率较高,生长较迅速;遮光度90%条件下,白木香幼苗存活率最高,但生长较差;自然光( CK)条件下,白木香幼苗存活率低,生长慢。说明在白木香育苗过程中,适当的遮光能够促进白木香种子的萌发和苗木的生长。%Using the shading net to probe into the different shading degrees on the effects of seed germination and seedling growth of Aquilaria sinensis( Lour.) Glg.The results showed that seed germination in the earliest time,germination rate reached more than 80%on the 75%,50%of shading degrees;under the natural light (CK),germination rate was the lowest and germination time was the latest.Different shading degrees of seedling growth of A.sinensis had significantly effects,on the shading degree is 75% and 50%, seedling survival rate was higher,and growth was rapid;in the shading degree 90%,seedling survival rate was the highest,but poor growth;and under the natural light ( CK) ,seedling survival rate is low,slow growth.These instruct that appropriate shading can pro-mote seed germination and seedling growth in the breeding process of A.sinensis.

  18. Analysis of Essential Oil Compounds from Different Species Osmanthus fragrans Lour. by Gas Chromatography-Mass Spectrometry%不同品种桂花挥发油成分的GC-MS分析

    Institute of Scientific and Technical Information of China (English)

    陈虹霞; 王成章; 孙燕

    2012-01-01

    采用水蒸气蒸馏法提取了江西地区金桂、银桂和四季桂3种桂花的挥发油,通过气相色谱-质谱联用法分析鉴定了3种桂花挥发油的化学成分及其含量。金桂挥发油中的主要成分是氧化芳樟醇、香叶醇、α-紫罗兰酮、β-紫罗兰酮、二氢猕猴桃内酯和邻苯二甲酸二丁酯等;主要香气成分为香叶醇、α-紫罗兰酮、β-紫罗兰酮、丁位癸内酯和芳樟醇及其氧化物。银桂挥发油的主要成分是氧化芳樟醇、环氧芳樟醇、丁位癸内酯、β-紫罗兰醇、β-紫罗兰酮、二氢猕猴桃内酯、邻苯二甲酸二异丁酯和邻苯二甲酸二丁酯等;主要香气成分为β-紫罗兰醇、β-紫罗兰酮、丁位癸内酯和氧化芳樟醇。四季桂挥发油的主要成分是香叶醇、茶香螺烷、β-紫罗兰醇、4-(2,6,6-三甲基-环己-1-己烯)-丁烷-2-醇、邻苯二甲酸二丁酯和丁位癸内酯等;主要香气成分为β-紫罗兰醇、香叶醇和茶香螺烷。%The essential oils from 3 varieties Osmanthusfragrans Lour. in Jiang Xi province were obtained by hydrodistillation and analysed by GC-MS. Main compounds of O. fragrans var. thunbergii were linalool oxide, geraniol, α-ionone, β-ionone, dihydroactinidiolide, butyl phthalate, etc. Main aroma components were geraniol, α-ionone, β-ionone, δ-decalactone and linalool oxide. The compounds of O. fragrans were linalool oxide, epoxylinalol, decalactone, α-ionol, β-ionone, diisobutyl phthalate, etc. Main aroma components were β-ionone, diisobutyl phthalate and linalool oxide. The compounds of 0. fragrans var. semperflorens were geraniol, theaspirane, βionol, dihydroactinidiolide, decalactone, 4-( 2,6, 6-Trimethyl-cyclohex-1- enyl)-butan-2-ol, etc. Main aroma components were β-ionol, geraniol and theaspirane.

  19. 大孔吸附树脂分离纯化桂花总黄酮工艺研究%Study on the Isolation and Purification Process of Total Flavonoids from Osmanthus fragrans Lour.with Macroporous Absorption Resin

    Institute of Scientific and Technical Information of China (English)

    郁建生; 罗显华

    2012-01-01

    [目的]研究大孔吸附树脂分离纯化桂花总黄酮的工艺条件.[方法]以贵州产桂花为原料,以桂花总黄酮吸附量及回收率等为考察指标,选用AB-8型大孔吸附树脂对桂花总黄酮进行分离纯化,分别采用静态试验和动态试验等考察AB-8型大孔树脂对桂花总黄酮的分离纯化最佳工艺条件及效果.[结果]pH值、洗脱剂、温度、上柱液浓度、径高比、流速、总黄酮与树脂质量比等工艺条件对桂花总黄酮的吸附洗脱量和回收率等影响很大.AB-8型大孔树脂分离纯化桂花总黄酮最佳工艺条件为上柱液pH值4~5;洗脱剂为浓度70%乙醇,料液比为1:4(g/ml),上柱总黄酮质量与树脂质量比为1:9.4,上柱液总黄酮浓度为17.86 mg/ml,流速为2~ 3ml/min;冲洗杂质用水体积2~3 BV,流速为3~4 ml/min;径高比为1.5:21.6;温度升高,吸附量下降但洗脱率加大.[结论]优选出了大孔吸附树脂分离纯化桂花总黄酮工艺条件,为桂花总黄酮的工业化生产提供试验依据.%[Objective] To study the isolation and purification process of total flavonoids from Osmanthus fragrans Lour. With macroporous absorption resin. [Method] Taking the adsorption capacity and recovery of total flavonoids as index, the optimum the isolation and purification process of total flavonoids from Guizhou O. Fragrans were respectively investigated by static test and dynamic test with AB-8 macroporous absorption resin. [Result] pH, eluant, temperature, concentration of column-loading sample, diameter-height ratio, current velocity and quality ratio of total flavonoids and absorption resin had great effects on the adsorption capacity and recovery of total flavonoids from O. Fragrans. The optimum conditions for extracting and purifying total flavonoids in static test were; the AB-8 type macroporous resin was blended with 4 times of pH 4-5sample solution (solid-liquid ratio of 1 :4 g/ml) together and then stirred to make it

  20. Effect on Soil Nutrients of Planting Polygonatum sibiricum Red., Fallopia multilfora (Thunb.) and Asparagus cochinchinensis (Lour.) Merr. Under Forest%林下种植黄精、何首乌及天门冬对土壤养分的影响

    Institute of Scientific and Technical Information of China (English)

    何银忠; 王有兵; 严毅; 王亚婷; 周庆宏

    2014-01-01

    为了了解林下种植黄精、何首乌及天门冬对林地土壤养分的影响,为以后大规模发展林下中药材种植提供一定的理论指导,研究黄精等3种中药材根际土壤的养分变化。结果表明,黄精能够使根际土壤酸化,对照土壤中有机质含量较低,仅为34.0 g/kg,无法满足植物的生长需求。由于受有机质含量的影响,水解性氮总体上也偏低,有效磷和速效钾基本上能够满足这3种药材的生长。由于针叶难以腐化成有机质,今后应该选择阔叶林地或混交林地作为种植地,也可以通过人工增加土壤有机质含量来改良土壤性质。%Effects on soil nutrients of planting Polygonatum sibiricum Red., Fallopia multiflora (Thunb.) and Asparagus cochinchinensis (Lour.) Merr. under forest was studied. The aim of this study was to provide some theoretical guidance for after large-scale planting of Chinese herbal medicine under forest. Polygonatum sibiricum Red. can make the rhizosphere soil acidification. The low organic matter content in control soil of only 34.0g/kg is unable to meet the plant growth demand. Because of the influence of organic matter content, hydrolyzable nitrogen is generally low too, and available phosphorus and available potassium basically can satisfy the growth of these 3 kinds of herbs. Because the needles were difficult to decay into organic matter, so we should choose broad-leaved forest or mixed forest as planting fields. Also we can increase the content of soil organic matter artificially to improve the soil properties.

  1. 紫茎泽兰乙醇提取物对五龄思茅松毛虫肠道细菌的抑制作用%Inhibition Effects of Ethanol Extracts from Eupatorium adenophorum Spreng on Intestinal Bacteria from 5th Instar Larvae of Dendrolimus kikuchii

    Institute of Scientific and Technical Information of China (English)

    朱丽丽; 刘绍雄; 王金华; 李彪; 张敬宜; 熊智

    2012-01-01

    以思茅松毛虫五龄幼虫肠道细菌为供试菌,采用牛津杯法测定紫茎泽兰不同部位乙醇提取物的抑菌效果.结果表明:紫茎泽兰不同部位乙醇提取物除茎提取物外,对5种思茅松毛虫肠道细菌(共10株)均有不同程度的抑制作用,抑制作用大小顺序为叶>根>茎.在相同质量浓度下,叶提取物对5种肠道细菌的抑菌作用大小顺序为雷金斯堡约克氏菌(Yokenella regensburgei)>弗氏柠檬酸杆菌(Citrobacter freundii)>产酸克雷伯氏杆菌(Klebsiella oxytoca)>克雷伯氏杆菌(K lebsiella peneumoniae)>苏云金芽孢杆菌(Bacillus thuringiensis),其对雷金斯堡约克氏菌、产酸克雷伯氏杆菌以及弗氏柠檬酸杆菌的最低抑菌质量浓度为0.125 g/mL,对其余2种细菌的最低抑菌质量浓度分别为0.25 g/mL、0.5 g/mL.根提取液对雷金斯堡约克氏菌和克雷伯氏杆菌无抑制作用,在相同质量浓度下,其对其余3种细菌的抑制作用大小顺序与叶提取液相同,最低抑菌质量浓度为0.25 g/mL.紫茎泽兰乙醇提取物具有良好的抑菌效果,可用于开发新型的植物源生物农药.%The Oxford cup method was used to measure the inhibition effects of ethanol extracts from different parts of Eupatorium adenophorum Spreng on intestinal bacteria from 5th instar larvae of Dendrolimu kikuchii. The results showed that except stems the ethanol extracts from leaves and roots of E. Adenophorum exhibited inhibition effects on 10 strains of intestinal bacteria and the effect order of different parts was leaves>roots>stems. Under the same concentration of leaf extracts, the order of inhibition effects on intestinal bacteria was Yokenella regensburgei^> Citrobacter freundii^> Klebsiella oxytoca^>Klebsiella peneumoniae~>Bacillus thuringiensis. The minimum inhibition concentration (MIC) was 0. 125 g/mL on Yokenella regensburgei,Klebsiella oxytoca and Citrobacter freundii ,0. 25 and 0. 5 g/mL on other 2 bacterial species

  2. Trachylobane and kaurane diterpenes from Croton floribundus spreng

    OpenAIRE

    Paula Karina S. Uchôa; José Nunes da Silva Jr.; Edilberto Rocha Silveira; Mary Anne S. Lima; Raimundo Braz-Filho; Letícia Veras Costa-Lotufo; Ana Jérsia Araújo; Manoel Odorico de Moraes; Claudia do Ó Pessoa

    2013-01-01

    A new trachylobane diterpene ent-trachyloban-18,19-diol (1) was isolated from root bark of Croton floribundus, along with known diterpenes ent-trachyloban-19-oic acid (2), 15b-hydroxy-ent-trachyloban-19-oic acid (3), ent-trachyloban-19-ol (4), ent-kaur-16-en-19-oic acid (5), ent-kaur-16-ene-6a,19-diol (6) and ent-16a-hydroxykaur-11-en-19-oic acid (7). ent-trachyloban-18,19-diol (1) was submitted to derivatization reactions affording four new compounds (8-11). Cytotoxic activity of diterpenes ...

  3. Trachylobane and kaurane diterpenes from Croton floribundus spreng

    Directory of Open Access Journals (Sweden)

    Paula Karina S. Uchôa

    2013-01-01

    Full Text Available A new trachylobane diterpene ent-trachyloban-18,19-diol (1 was isolated from root bark of Croton floribundus, along with known diterpenes ent-trachyloban-19-oic acid (2, 15b-hydroxy-ent-trachyloban-19-oic acid (3, ent-trachyloban-19-ol (4, ent-kaur-16-en-19-oic acid (5, ent-kaur-16-ene-6a,19-diol (6 and ent-16a-hydroxykaur-11-en-19-oic acid (7. ent-trachyloban-18,19-diol (1 was submitted to derivatization reactions affording four new compounds (8-11. Cytotoxic activity of diterpenes 1, 3, 4, 7-11 against three human cancer cell lines was evaluated. No compounds showed cytotoxic potential with IC50 values greater than 25 mg/mL. Compound 6 was evaluated against five human cancer cell lines, showing moderate effect against three cancer cell lines, MDA-MB-435, HCT-8 and HCT-116, with IC50 values of 14.32, 13.47 and 12.1 mg/mL, respectively.

  4. Trachylobane and kaurane diterpenes from Croton floribundus spreng

    Energy Technology Data Exchange (ETDEWEB)

    Uchoa, Paula Karina S.; Silva Junior, Jose Nunes da; Silveira, Edilberto Rocha; Lima, Mary Anne S., E-mail: mary@dqoi.ufc.br [Departamento de Quimica Organica e Inorganica, Centro de Ciencias, Universidade Federal do Ceara, Fortaleza, CE (Brazil); Braz-Filho, Raimundo [Laboratorio de Ciencias Quimicas, Centro de Ciencias Tecnologicas, Universidade Estadual do Norte Fluminense, Campo dos Goytacazes, RJ (Brazil); Costa-Lotufo, Leticia Veras; Araujo, Ana Jersia; Moraes, Manoel Odorico de; Pessoa, Claudia do O [Departamento de Farmacologia e Fisiologia, Centro de Ciencias da Saude, Universidade Federal do Ceara, Fortaleza, CE (Brazil)

    2013-09-01

    A new trachylobane diterpene ent-trachyloban-18,19-diol (1) was isolated from root bark of Croton floribundus, along with known diterpenes ent-trachyloban-19-oic acid (2), 15{beta}-hydroxy-ent-trachyloban-19-oic acid (3), ent-trachyloban-19-ol (4), ent-kaur-16-en-19-oic acid (5), ent-kaur-16-ene-6{alpha},19-diol (6) and ent-16{alpha}-hydroxykaur-11-en-19-oic acid (7). ent-trachyloban-18,19-diol (1) was submitted to derivatization reactions affording four new compounds (8-11). Cytotoxic activity of diterpenes 1, 3, 4, 7-11 against three human cancer cell lines was evaluated. No compounds showed cytotoxic potential with IC{sub 50} values greater than 25 {mu}g/mL. Compound 6 was evaluated against five human cancer cell lines, showing moderate effect against three cancer cell lines, MDA-MB-435, HCT-8 and HCT-116, with IC{sub 50} values of 14.32, 13.47 and 12.1 {mu}g/mL, respectively. (author)

  5. Three New Apianen lactones Compounds from Dioscorea Cirrhosa Lour

    Institute of Scientific and Technical Information of China (English)

    ZHENG ShangZhen; GUO Zhen; SHEN Tong; ZHEN XuDong

    2001-01-01

    @@ Dioscorea Cirrhosa Laour. which is a species of the Dioscorea, grows abundantly in southern and eastern China. Its roots are used in Chinese medicine as a remedy for various diseases, especially use for tumours and cancers. These results have prompted a search for its chemical constituents. Continuing out previous work,further studies on the constituents of the drug yielded three new compounds along with three known compounds in the EtOAc Fr of EtOH extraction.

  6. Biological activity of fucoidan from leafy bladderwort (Utricularia aurea Lour.)

    OpenAIRE

    Chotigeat, W.; Phongdara, A.; Choosawad, D.

    2005-01-01

    Fucoidan extracted from aquatic plant Utricularia aurea was 1.3% of dry weight and comprised glucuronic acid 62.5% and fucose 4.98% of the crude extract and 28.74% of the fucose was sulfate. The fucoidan was investigated for antibacterial activity. The minimal inhibitory concentration (MIC) of crude fucoidan against Vibrio harveyi and Escherichia coli was 20 and 10 mg/ml. Moreover, the fucoidan had anticoagulant activity. The crude fucoidan had activated partial thromboplastin time (APTT) at ...

  7. Biological activity of fucoidan from leafy bladderwort (Utricularia aurea Lour.

    Directory of Open Access Journals (Sweden)

    Chotigeat, W.

    2005-12-01

    Full Text Available Fucoidan extracted from aquatic plant Utricularia aurea was 1.3% of dry weight and comprised glucuronic acid 62.5% and fucose 4.98% of the crude extract and 28.74% of the fucose was sulfate. The fucoidan was investigated for antibacterial activity. The minimal inhibitory concentration (MIC of crude fucoidan against Vibrio harveyi and Escherichia coli was 20 and 10 mg/ml. Moreover, the fucoidan had anticoagulant activity. The crude fucoidan had activated partial thromboplastin time (APTT at 4.47 IU/mg.

  8. Use of Medical Plants in Schools Communities from Sinop, Mato Grosso.

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    A. C. M. Urtado

    2013-03-01

    Full Text Available Abstract: This study was conducted in Sinop, Mato Grosso, on two school communities. It was applied semi-structured questionnaires with questions focused on socioeconomic and the use of medicinal plants. It has as finality proved the effective use of medicinal plants on the everyday and a levy of the most used plant. The general profile of the respondents has shown that the women detain the major part of the knowledge, and that pass this uses to the future generations and friends, and find these plants on specialty stores, backyards, supermarket, root stores, bush and fairs. The plants that were found more frequently was (Ruta graveolens L., Babosa (Aloe vera L., Erva-Cidreira (Lippia alba Mill., Erva-Santa-Maria (Chenopodium ambrosioides L., Boldo (Plectranthus amboinicus Spreng., Hortel(Menta x vilosa Huds. e Terramicina (Alternanthera dentata Moench..Keywords: medical plants, Sinop, school.

  9. Estudo da interferência de óleos essenciais sobre a atividade de alguns antibióticos usados na clínica Study of the interference of essential oils on the activity of some antibiotic used clinically

    OpenAIRE

    Rinalda A. Guerra de Oliveira; Edeltrudes de O. Lima; Wellington L. Vieira; Kristerson R. Luna Freire; Vinicius N. Trajano; Igara O. Lima; Souza, Evandro L.; Manuella S. Toledo; Raimundo N. Silva-Filho

    2006-01-01

    O objetivo deste estudo foi investigar a interferência dos óleos essenciais de Lippia sidoides Cham, Plectranthus amboinicus Lour Spr., Conyza bonariensis L. e Eucalyptus citriodora Hook sobre o efeito de antibióticos utilizados na clínica. Os ensaios foram realizados com ampicilina (10 µg/mL), cefalotina (30 µg/mL), cloranfenicol (30 µg/mL), gentamicina (10 µg/mL) e tetraciclina (30 µg/mL) isolados e em associação com os óleos essenciais (4% v/v) através do método de difusão em meio sólido u...

  10. Propagation by cuttings of the rhizome of Aspilia montevidensis (Spreng. KuntzePropagação por estacas de rizoma de Aspilia montevidensis (Spreng. Kuntze

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    Angeline Martini

    2013-03-01

    Full Text Available The indication for use of native species in the landscape has become increasingly more frequent, but find them on the market still is not easy. One reason for this is the difficulty in production, which begins in the processes of propagation. Effective methods of spreading Aspilia montevidensis are not yet known. Thus the objective of this study was to vegetative propagation by cuttings of the rhizome of Aspilia montevidensis in different seasons. Two treatments were tested in each season: T1 – section of the tip of the rhizome and T2– section of the base of the rhizome, with three repetitions each of nine sections in the spring and summer and three replicates of ten sections each fal and winter. The variables were: percentage of rooted cuttings, dead and living, number of roots per cutting, number and length of shoots in each stake. Statistical analysis was performed by the SNK test at 95% probability. There were significant differences between treatments for the percentage of live cuttings in summer and during autumn for the percentage of rooted cuttings (T1= 30.7 % and T2= 0 %, living and the number of roots. In the other treatments and stations there were no statistical differences, although the highest rate of rooting was found in summer (T1 = 73.6 % and T2 = 38.15 %. We conclude that the season is a factor that influences the propagation by cuttings of rhizomes of Aspilia montevidensis A indicação do uso de espécies nativas no paisagístico tem se tornado a cada dia mais freqüente, porém encontrá-las no mercado ainda não é tarefa fácil. Um dos motivos para isso é a dificuldade na produção, que se inicia nos processos de propagação. Métodos eficazes de propagação de Aspilia montevidensis ainda não são conhecidos. Desta forma o objetivo desse trabalho foi realizar propagação vegetativa por estacas de rizoma de Aspilia montevidensis em diferentes estações do ano. Testaram-se dois tratamentos em cada estação: T1 – secção apical do rizoma e T2 – secção basal do rizoma, com três repetições de nove secções cada na primavera e verão e três repetições de dez secções cada no outono e inverno. As variáveis analisadas foram: percentagem de estacas enraizadas, vivas e mortas; número de raízes por estaca; número e comprimento das brotações em cada estaca. A análise estatística foi realizada pelo teste SNK a 95% de probabilidade. Houve diferença significativa entre os tratamentos para a percentagem de estacas vivas no período de verão, e no período de outono para a percentagem de estacas enraizadas (T1 = 30,7% e T2 = 0%, vivas e o número de raízes. Nos demais tratamentos e estações do ano não se verificaram diferenças estatísticas, embora a maior taxa de enraizamento tenha sido encontrada no verão (T1 = 73,6% e T2 = 38,15%. Conclui-se que a estação do ano é um fator que influência na propagação vegetativa, por estacas de rizomas apicais de Aspilia montevidensis.

  11. Hypnotic effect of ecdysterone isolated from Pfaffia glomerata (Spreng. Pedersen Efeito hipinótico de ecdisterona isolada de Pfaffia glomerata (Spreng. Pedersen

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    Raquel Fenner

    2008-06-01

    Full Text Available In this study the depressant effect of fractions from P. glomerata was initially evaluated using the mice barbiturate sleeping time test as reference. The fractions tested were the CHCl3, the EtOAc, the n-BuOH and the aqueous fraction obtained from P. glomerata subterraneous parts. Only the pretreatment with the lipophilic fraction (CHCl3: EtOAc, 1:1, w/w increased the barbiturate sleeping time (i.p 500 mg/kg; v.o. 1000 mg/kg. Ecdysterone, the main substance isolated from this lipophilic fraction, was identified by spectroscopic methods and its content in the ethanol extract was determined as 1.4% (w/w by HPLC. In order to investigate the hypothesis of ecdysterone displaying a depressant effect on nervous central system, an evaluation toward the hypnotic-sedative and anxiolytic effects of this drug was carried out. Ecdysterone 100 mg/kg, i.p, increased the barbiturate sleeping time without provoking hypothermia; when administered by oral route its minimal effective dose was 400 mg/kg. On the other hand, ecdysterone (100 mg/kg, i.p; 400 mg/kg, p.o did not impair motor coordination and was ineffective on pentylenetetrazole-induced convulsion, elevated plus-maze and step-down inhibitory avoidance tests, indicating that at these doses the drug does not present an anxiolytic profile and does not cause manifest neurotoxic effects as well. In conclusion, the lipophilic fraction from P. glomerata presents a hypnotic effect being ecdysterone one of the compounds responsible for this CNS activity.Neste trabalho foi avaliado, em roedores, o efeito depressor das frações clorofórmio (CHCl3, acetato de etila (EtOAc e n-butanol, obtidas das partes subterrâneas de Pfaffia glomerata, empregando-se o teste de tempo de sono barbitúrico como referência. Somente a fração lipofílica (CHCl3:EtOAc, 1:1, m/m (i.p. 500 mg/kg; v.o. 1000 mg/kg potenciou o tempo de sono induzido por pentobarbital. A ecdisterona foi isolada e identificada como constituinte majoritário (1,4% m/m desta fração, através de cromatografia líquida de alta eficiência e métodos espectroscópicos, respectivamente. Este composto potenciou o tempo de sono barbitúrico (100 mg/kg, i.p.; 400 mg/kg, v.o, sem causar hipotermia. Nestas mesmas doses, a ecdisterona não alterou a performance dos animais no rota-rod, esquiva inibitória e labirinto em cruz-elevado, além de não alterar o padrão de convulsões induzidas por pentilenotetrazol. Este perfil indica que esta substância, nestas doses, não apresenta perfil ansiolítico ou neurotóxico. Estes resultados indicam que a ecdisterona é o componente responsável pela ação hipnótica apresentada pela fração lipofílica obtida das partes subterrâneas de P. glomerata.

  12. Semeadura direta de canafístula (Peltophorum dubium (Spreng.taub. No enriquecimento de capoeiras Direct seeding of canafistula (Peltophorum dubium (Spreng. taub. To regenerate shrubs

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    Vilmar Luciano Mattei

    2002-11-01

    Full Text Available O presente trabalho teve por objetivo avaliar o comportamento da canafistula (Peltophorumdubium, em semeadura direta a campo, em área de capoeira originada após o abandono do local utilizado com cultivos agrícolas sucessivos. No local foram roçadas faixas de 1,5 m, a cada 3 m. A semeadura foi realizada em setembro de 1997. Visando proteger os pontos semeados, foram utilizados diferentes tipos de protetores (copos de plástico e copos de papel, sem fundo e laminado de madeira, fixados sobre os pontos semeados com três sementes cada. As sementes foram escarificadas mecanicamente a 1.725 rpm, durante 60 segundos, com lixa Norton 60. O delineamento foi em blocos casualizados, com seis repetições. As variáveis avaliadas foram emergência, sobrevivência, número de pontos com plantas, densidade a 1 ano e altura das plantas aos 18 meses. Os resultados obtidos aos nove meses demonstraram que a utilização de qualquer um dos protetores físicos contribuiu para o estabelecimento de plantas em mais de 80% dos pontos semeados, e em mais de 75%, um ano após a semeadura, quando utilizado o laminado, o que indica que a semeadura direta é uma alternativa de implantação para a espécie, possibilitando transformar as áreas de capoeiras em um sistema agroflorestal ou mesmo silvipastoril, no futuro.This work aimed to evaluate the development of Peltophorum dubium by direct seeding on a succesively cropped field. Seeding was performed in rows of 1.5 m, three meters apart, on September 1977. To protect the seed-spots shelters, bottomless paper and plastic cups were used and wood slates were fixed on them. Seeds were mechanically scarified for 60 seconds. The variables evaluated were emergence, seedling survival, number of seed-spots with plants plant density at one year and plant height at the 18th month. Results at the 9th month showed that the seed-spot shelters contributed for plant establishment in 80% of the seed-spots and in more than 75% of them one year after seeding, when wood slates were used. Direct seeding is a viable alternative for this species which will allow the transformation of shrub areas into an agroforest or even silvipastural system in the near future.

  13. Isolation and prebiotic activity of inulin-type fructan extracted from Pfaffia glomerata (Spreng) Pedersen roots.

    Science.gov (United States)

    Caleffi, Edilainy Rizzieri; Krausová, Gabriela; Hyršlová, Ivana; Paredes, Larry Ladislao Ramos; dos Santos, Marcelo Müller; Sassaki, Guilherme Lanzi; Gonçalves, Regina Aparecida Correia; de Oliveira, Arildo José Braz

    2015-09-01

    Pfaffia glomerata (Amaranthaceae) is popularly known as "Brazilian ginseng." Previous studies have shown that fructose is the major carbohydrate component present in its roots. Inulin-type fructans, polymers of fructose, are the most widespread and researched prebiotics. Here, we isolated and chemically characterized inulin extracted from P. glomerata roots and investigated its potential prebiotic effect. Fructans were isolated and their structures were determined using colorimetric, chromatography, polarimetry, and spectroscopic analysis. The degree of polymerization (DP) was determined, and an in vitro prebiotic test was performed. The structure of inulin was confirmed by chromatography and spectroscopic analysis and through comparison with existing data. Representatives from the genera Lactobacillus and Bifidobacterium utilized inulin from P. glomerata, because growth was significantly stimulated, while this ability is strain specific. The results indicated that inulin extracted from P. glomerata roots represents a promising new source of inulin-type prebiotics.

  14. MODELO DE INCREMENTO PARA ÁRVORES SINGULARES – Nectandra megapotamica (Spreng. Mez

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    Peter Spathelf

    2004-05-01

    Full Text Available No setor florestal, os modelos de crescimento e incremento são utilizados para predizer o crescimento de uma árvore, de um povoamento ou de uma floresta. Nas florestas heterogêneas e de várias idades do Rio Grande do Sul, modelos de árvores individuais são apropriados. O presente trabalho foi estabelecido para contribuir para a formulação de um modelo de incremento de uma espécie da Floresta Estacional Decidual do Rio Grande do Sul: a Nectandra megapotamica. A coleta de dados foi realizada no distrito de Vale Vêneto, próximo a Santa Maria, nos anos de 1994 e 1997. A vegetação foi classificada em diferentes estágios sucessionais e nesses estágios, instaladas unidades amostrais permanentes (uap. Em cada uap, todas as árvores com DAP ≥ 5 cm foram identificadas, numeradas e medidas. Adicionalmente, a posição sociológica e variáveis do sítio foram estimadas. Em cada uap, calcularam-se a área basal individual, área basal por ha e área basal das árvores maiores que a concorrida (BAL. Em seguida, estabeleceram-se funções de crescimento utilizando o procedimento stepwise. A fórmula geral do modelo é: Incremento (g/ano = f (Dimensões, Concorrência, Sítio. O melhor modelo para Nectandra megapotamica é: ICA = 0,002084 + 0,000001039 x DAP² – 0,0003498 x Degradação – 0,000375 x P.S. – 0,00000904 x Pedregosidade – 0,0000103 x BAL.

  15. Effects of maturity on physicochemical properties of Gac fruit (Momordica cochinchinensis Spreng.).

    Science.gov (United States)

    Tran, Xuan T; Parks, Sophie E; Roach, Paul D; Golding, John B; Nguyen, Minh H

    2016-03-01

    The aril around the seeds of Gac fruit is rich in fatty acids and carotenoids (lycopene and β-carotene). Understanding how these qualities are affected by fruit maturity at harvest may identify indices for quality assessment. Some physical and chemical properties of Gac fruit were determined for fruit harvested between 8 and 16 weeks after pollination (WAP). Fruit respiration rates and ethylene production rates were assessed after harvest and up to 20 days in storage at 20°C. Fruit harvested at 14 WAP had the highest oil (0.27 ± 0.02 g/g DW), lycopene content (0.45 ± 0.09 mg/g FW), and β-carotene content (0.33 ± 0.05 mg/g FW) which declined by 16 WAP. External skin color and aril TSS were indicative of oil and carotenoid contents in aril. Skin color, TSS and potentially firmness were good indices of fruit quality. Harvesting less mature fruit at 12 WAP would be practical as the fruit were firmer and more capable of transport; however, quality during postharvest ripening may be limited. Fruits continued to ripen after they were harvested and an ethylene peak in the least mature fruit may reflect a climacteric behavior but this needs further investigation. PMID:27004120

  16. Phytochemical and antimicrobial screening of Globimetula oreophila (Oliv van Tiegh and Phragmanthera capitata (Spreng Balle

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    O O Ogbole

    2013-01-01

    Full Text Available Background: Medicinal plants will continue to be relevant in the production of lead compounds that can be utilized for its antibacterial activity directly or through chemical modification (s. Aims: The present study is conducted to evaluate the antibacterial activity vis-ΰ-vis phytochemical constituents of Globimetula oreophila and Phragmanthera capitata. Materials and Methods: Cup plate agar diffusion assay was used to determine the minimum inhibitory concentrations (MIC of the extracts of both plants against Escherichia coli, Klebsiella spp., Shigella spp., Salmonella typhi, Staphylococcus aureus and Pseudomonas aeruginosa by plotting the diameters of zones of inhibition against the logarithm of concentrations and the straight line graph so generated extrapolated to the value equivalent to the diameter of the cork-borer. The antilog of the corresponding value of concentration was taken as the MIC value. Statistical Analysis: All the values of the results of cup plate diffusion assay were expressed as means of two replicates ± standard deviation. Results: Preliminary phytochemical screening reveals the presence of saponin, tannins, alkaloids, anthraquinones and flavonoid in both plants. Antibacterial screening of both plants showed that both of them were active against all clinical bacterial isolates tested with P. capitata having a higher activity than G. oreophila on most of the isolates. The ability of both plants to inhibit the growth of S. typhi is a major breakthrough in the control of incidence of typhoid fever notwithstanding the reports of resistance to existing antibiotics. Conclusion: It therefore follows that the importance of both plants in the management and control of infections with which all the bacterial isolates used for this study were associated cannot be underestimated.

  17. Evaluation of antioxidant and anti-inflammatory activity of Euphorbia heyneana Spreng

    Institute of Scientific and Technical Information of China (English)

    Ganga Rao Battu; Sambasiva Rao Ethadi; Veda Priya G; Swathi Priya K; Chandrika K; Venkateswara Rao A; Satya Obbala Reddy

    2011-01-01

    Objective:To assess the antioxidant and anti-inflammatory activities of the alcoholic extract of Euphorbia heyneana (E. heyneana) in carrageenan induced inflammation in rats. Methods:In vitro antioxidant activity was evaluated for superoxide radical, hydroxyl radical and DPPH radical scavenging activity. Three doses 200, 400 and 800 mg/kg were tested for anti-inflammatory activity in carrageenan induced rat paw oedema model and paw thickness was measured every one hour up to 6 hours. Results:The alcoholic extract of E. heyneana produced dose dependent inhibition of superoxide radical, hydroxyl radical and DPPH radicals. In carrageenan induced inflammation model, all three doses produced significant percentage inhibition of rat paw oedema and 800 mg/kg dose produced maximum percent inhibition of rat paw oedema (47.06%) among the three doses compared to control group. Conclusions:It can be concluded that alcoholic extract of E. heyneana shows good in vitro antioxidant and in-vivo anti-inflammatory activities in rats.

  18. In vitro establishment of Peltophorum dubium ((Spreng. Taub. in function of concentrations of the MS medium

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    Andressa Vasconcelos Flôres

    2011-12-01

    Full Text Available The objective of this study was to evaluate the influence of several concentrations of MS medium on in vitro establishment of Peltophorum dubium shoot apical segments (80-100 mm isolated from seedlings germinated under aseptic conditions. The salts concentrations 50, 75, 100, 125 and 150% of MS medium, were supplemented with 30 g L-1 sucrose, 100 mg L-1 of myo-inositol, 7 g L-1 agar and pH adjusted to 5.7. The experimental design was completely randomized, with 20 replications, each consisting of a vial containing one explant. The cultures were maintained for 60 days in culture room under 16-hour photoperiod, light intensity of 20 μmol m-2 s-1 and temperature of 25±2°C. There was no effect of different concentrations of MS medium for most variables. High average of in vitro survival (98% and establishment (98% were observed. The largest number of leaves (2.8 occurred at a dilution of 75%, decreasing from this point. At the same concentration the highest callus induction was observed, which was decreasing until not be observed under 125% of original composition; at 150% there was, again, the presence of calli. It was not observed rooting of explants during in vitro cultivation. Considering the set of variables and, additionally, that the presence of calli is not desirable, it is recommended the use of the original concentration of salts from the MS nutrient medium for the in vitro establishment of P. dubium shoot apicals segments.

  19. Seasonal influence on the essential oil production of Nectandra megapotamica (Spreng. Mez

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    Lúcio de Paula Amaral

    2015-02-01

    Full Text Available This study evaluated the seasonal influence on the yield and chemical composition of the essential oil (EO of Nectandra megapotamica. Fresh young (YL and old leaves (OL obtained from three trees in each season (Nov/2010 to Sep/2011 collected in Santa Maria-RS were hydrodistilled in triplicate. The chemical composition was determined by the gas chromatography coupled to mass spectrometry (GC-MS and the yield on dry basis was evaluated by two-way ANOVA (seasons, development stage. Spring (Sp and summer (Su showed higher average incomes (0.45 and 0.33%, which occurred when flowering, fruiting, and growth of YL and senescence of OL took place, while autumn (Au presented the lowest yield (0.25% during the rustification of OL. The highest yield was obtained for the YL in Sp (0.59% and the lowest for the OL in Au (0.21%. The major constituents of the EO were independent from the season and were identified as α-pinene, bicyclogermacrene, β-pinene, germacrene D, and limonene. Seasonality and phenology influenced the production of EO probably due to morphological and metabolic alterations in the leaves as well as due to the needs of the tree, such as attraction and/or protection.

  20. Antioxidant properties of Lippia javanica (Burm.f.) Spreng. / C. Pretorius

    OpenAIRE

    Pretorius, Corlea

    2010-01-01

    The evolution of aerobic metabolic processes unavoidably led to the production of reactive oxygen species (ROS). ROS have the ability to cause harmful oxidative damage to biomolecules. Increased ROS generation and subsequent oxidative stress have been associated with aging and neurodegenerative disorders such as Parkinson’s and Alzheimer’s diseases as a result of the extreme sensitivity of the central nervous system to damage from ROS. Antioxidant defence systems have co–evolve...

  1. Old sleeping Sicilian beauty: seed germination in the palaeoendemic Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae).

    Science.gov (United States)

    De Castro, O; Gianguzzi, L; Carucci, F; De Luca, A; Gesuele, R; Guida, M

    2015-09-01

    Petagnaea gussonei (Apiaceae) is a perennial herbaceous species endemic to northeast Sicily (Nebrodi Mountains). It is considered a remnant of the Sicilian Tertiary flora, and is endangered according to the Red List. There is no information in the literature about the germinability of its seeds, even though seed production is know to occur. The aim of this study was to obtain data to better understand seed germination of this species and its biological implications. Thus, several approaches were employed: vitality analyses, gibberellic acid supply, germination and soil microbial flora analyses via end-point and qPCR. The results suggest that seed germination occurs after ca. 1.5 years at a rate of ca. 11%. The seeds can be classified as physiologically dormant, and probably require prolonged cold stratification for germination. Because seed germination is low, it is likely that agamic reproduction represents an important mean for its conservation and survival. These results have important implications for P. gussonei survival and should be considered in possible re-introduction attempts aimed at restoring threatened populations. PMID:25847095

  2. Old sleeping Sicilian beauty: seed germination in the palaeoendemic Petagnaea gussonei (Spreng.) Rauschert (Saniculoideae, Apiaceae).

    Science.gov (United States)

    De Castro, O; Gianguzzi, L; Carucci, F; De Luca, A; Gesuele, R; Guida, M

    2015-09-01

    Petagnaea gussonei (Apiaceae) is a perennial herbaceous species endemic to northeast Sicily (Nebrodi Mountains). It is considered a remnant of the Sicilian Tertiary flora, and is endangered according to the Red List. There is no information in the literature about the germinability of its seeds, even though seed production is know to occur. The aim of this study was to obtain data to better understand seed germination of this species and its biological implications. Thus, several approaches were employed: vitality analyses, gibberellic acid supply, germination and soil microbial flora analyses via end-point and qPCR. The results suggest that seed germination occurs after ca. 1.5 years at a rate of ca. 11%. The seeds can be classified as physiologically dormant, and probably require prolonged cold stratification for germination. Because seed germination is low, it is likely that agamic reproduction represents an important mean for its conservation and survival. These results have important implications for P. gussonei survival and should be considered in possible re-introduction attempts aimed at restoring threatened populations.

  3. The selects of bearberry (Arctostaphylos uva-ursi (l. spreng. from natural populations in Poland

    Directory of Open Access Journals (Sweden)

    Hanna Malinowska

    2014-02-01

    Full Text Available Five plants of bearberry selected from natural stands in Poland and one from a Botanical Garden were propagated by cuttings. The clonal plants were first kept under observation for two years planted on different substrates in containers and were afterwards field cultivated for three subsequent years. The existence of a significant differentiation within these Arctostaphylos uva-ursi clones was proved. Two selects of good ornamental features as well as with high concentration of medicinal substance - arbutin were chosen. Plants started fruiting in the fourth year of growth and the area covered by a single plant exceeded 2 m2 after five years of cultivation.

  4. Antioxidant, analgesic and anti-inflammatory activities of Leucas cephalotes (Roxb.ex Roth) Spreng

    OpenAIRE

    Bhukya Baburao; Anreddy Rama Narsimha Reddy; Gangarapu Kiran; Yellu Narsimha Reddy; Gottumukkala Krishna Mohan

    2010-01-01

    The whole plant of the methanolic extract from Leucas cephalotes was screened for invitro antioxidant (using the DPPH method), invivo analgesic (using hot plate test in mice) and anti-inflammatory (using rat paw edema test) activities. The methanolic extract of Leucas cephalotes (MELC) scavenged the DPPH radicals in a dose-dependent manner. The IC50 value to scavenge DPPH radicals was found to be 421.3µg/ml. A significant (p

  5. POPULATION STRUCTURE AND REGENERATION POTENCIALITY OF Attalea phalerata Mart. ex Spreng. (ACURI

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    Raquel Rejane Bonato Negrelle

    2013-12-01

    Full Text Available http://dx.doi.org/10.5902/1980509812356Aiming to provide basis for the establishment of Attalea phalerata sustainable harvesting managementplan, the results of the evaluation of the population structure and its respective natural regeneration potentialare presented. Based on the obtained data, it was evaluated the potential capacity of fruit and oil yielding,related to the studied area. The research was performed in a 1ha plot representative of Acuri Dense Forest,located at RPPN SESC PANTANAL (Barão de Melgaço Mun., Mato Grosso state, Brazil. A total of1164 individuals were registered, being 462 representatives of reproductive adults and 209 representativesof immature adults. 123 individuals were included as young and 370 as seedlings. The population hadan inverted- J age structure, characteristic of stable populations with constant regeneration potentiality.The minimum immediate potential productivity was ca. 323 up to 970 Kg ha-1 for the mesocarp oil and370 up to 601 Kg ha-1 for the kernel oil. The maximum immediate potential productivity was ca. 970 up to2910 Kg ha-1 for the mesocarp oil and 1109 up to 1802 Kg ha-1 for kernel oil.

  6. A new megastigmane diglycoside from Litsea glutinosa (Lour.) C. B. Rob

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Yun-Song; Huang, Rong; Zhang, Hong-Bing; Yang, Jing-Hua, E-mail: yangjh@ynu.edu.cn [Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University (China); Liao, Zhen [Yunnan Academy of Tobacco Science (China); Li, Yan [State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences (China)

    2011-09-15

    Phytochemical study on the leaves and twigs of afforded the new megastigmane diglycoside (6S, 7E, 9R)-6, 9-dihydroxy-4, 7-megastigmadien-3-one-9-O-[{alpha}-L-arabinofuranosyl-(1 ->6)]- {beta}-D-glucopyranoside (1), along with glycosides (6S, 7E, 9R)-roseoside (2), (7'R, 8'R)- 3, 5'-dimethoxy-9, 9'-dihydroxy-4, 7'-epoxylignan 4'-b-D-glucopyranoside (3), (7'R, 8'S)- dihydrodehydrodiconifenyl alcohol 9'-O-{beta}-D-xylopyranoside (4) and pinoresinol 3-O-{beta}-Dglucopyranoside (5). Their structures were established on the basis of extensive spectroscopic and chemical methods. Compounds 2-5 were reported for the first time in this species. Compound 1 was evaluated for cytotoxic activities against human tumor cell lines (myeloid leukemia HL-60, hepatocellular carcinoma SMMC-7721, lung cancer A-549, breast cancer MCF-7 and colon cancer SW480 cells), for which it was proved to be inactive (IC{sub 50} > 40 {mu}M). (author)

  7. Uji In vitro Aktivitas Antelmintik Ekstrak Etilasetat Daun Pugun Tanoh [Curanga fel-terrae (Lour.) Merr.

    OpenAIRE

    Sitepu, Maria Atrina

    2016-01-01

    Helminthiasis is a world health problem. Emerging of helminthical resistant strain to anthelmintics leads to the difficulty of helminthiasis treatment. Therefore, it is nessecary to do study to find out a new anthelmintic source. This study was to determine characteristics of dried material and ethylacetate extract of Curanga fel-terrae leaves, as well as the anthelmintic activity of the leaves extract. The extract was prepared by extracting C.fel-terrae dried leaves powder in ethylacetate...

  8. Comparison on characterization of longan (Dimocarpus longan Lour.) polyphenoloxidase using endogenous and exogenous substrates.

    Science.gov (United States)

    Sun, Jian; Zhang, Ezhen; Xu, Liangxiong; Li, Zhichun; Wang, Zhenxing; Li, Changbao

    2010-09-22

    Longan polyphenoloxidase (PPO) was extracted and partially purified using ammonium sulfate precipitation and dialysis. The PPO characterizations were compared using endogenous substrate (-)-epicatechin and exogenous substrate catechol. The optimal pH and optimal temperature for the PPO activity were different when reacting with both substrates. The addition of ethylenediaminetetraacetic acid disodium salt into both substrate-enzyme systems exhibited the same lowest inhibition of the PPO activity. L-ascorbic acid and L-cysteine were the best inhibitors to endogenous substrate-enzyme system, while L-ascorbic acid and glutathione were most effective inhibitors to exogenous substrate-enzyme system. Cupric (Cu2+), ferric (Fe3+), and ferrous (Fe2+) ions accelerated the enzymatic-catalyzed reactions of both substrates. Kinetic analysis indicated that longan PPO strongly bound endogenous substrate but possessed a higher catalytic efficiency to exogenous substrate, and moreover, (-)-epicatechin was determined as the optimal substrate for longan PPO. This study is useful to exactly illuminate the enzymatic-catalyzed browning mechanism of postharvest longan fruit.

  9. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour

    Directory of Open Access Journals (Sweden)

    Xiao-Jun Li

    2016-06-01

    Full Text Available A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC and 1D/2D-NMR spectra data. The backbone of BP1 consists of →6α-d-Glcp1→6,3α-d-Glcp1→(6α-d-Glcp1x-6,3α-d-Glcp1-(6α-d-Glcp1y→. The side chains were terminal α-d-Glcp1→ and α-d-Glcp1→ (6α-d-Glcp1z→4α-d-Glcp1→3α-d-Glcp1→4α-d-Glcp1→ (x + y + z = 5, which are attached to the backbone at O-3 of 3,6α-d-Glcp1. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects.

  10. Chemical Structure and Immunomodulating Activities of an α-Glucan Purified from Lobelia chinensis Lour.

    Science.gov (United States)

    Li, Xiao-Jun; Bao, Wan-Rong; Leung, Chung-Hang; Ma, Dik-Lung; Zhang, Ge; Lu, Ai-Ping; Wang, Shun-Chun; Han, Quan-Bin

    2016-01-01

    A neutral α-glucan, named BP1, with a molecular mass of approximately 9.45 kDa, was isolated from Lobelia chinensis by hot-water extraction, a Q-Sepharose Fast Flow column and Superdex-75 column chromatography. Its chemical structure was characterized by monosaccharide analysis, methylation analysis and analysis of its FT-IR, high performance gel permeation chromatography (HPGPC) and 1D/2D-NMR spectra data. The backbone of BP1 consists of →₆α-d-Glcp¹→6,3α-d-Glcp¹→(₆α-d-Glcp¹)x-6,3α-d-Glcp¹-(₆α-d-Glcp¹)y→. The side chains were terminal α-d-Glcp¹→ and α-d-Glcp¹→ (₆α-d-Glcp¹)z→₄α-d-Glcp¹→₃α-d-Glcp¹→₄α-d-Glcp¹→ (x + y + z = 5), which are attached to the backbone at O-3 of 3,6α-d-Glcp¹. The results of the effect of BP1 on mouse macrophage cell line RAW 264.7 indicate that BP1 enhances the cell proliferation, phagocytosis, nitric oxide production and cytokine secretion in a dose-dependent manner. Because the inhibitor of Toll-like receptor 4 blocks the BP1-induced secretion of TNF-α and IL-6, we hypothesize that α-glucan BP1 activates TLR4, which mediates the above-mentioned immunomodulating effects. PMID:27314319

  11. Effect of Rotula aquatica Lour. on ethylene-glycol induced urolithiasis in rats.

    Directory of Open Access Journals (Sweden)

    Christina A.J.M

    2011-03-01

    Full Text Available The effect of the alcoholic extract of Rotula aquatica (Boraginaceae against ethylene glycolinduced urolithiasis in albino rats is summarized in this study. Lithiasis was induced in rats by administrating 1% ethylene glycol in drinking water for 28 days and was manifested by high urinary calcium, phosphate, oxalate, protein, uric acid, creatinine and low urinary magnesium content. The alcoholic extract of Rotula aquatica was administered in 200 mg/kg body weight orally for 28 days along with 1% ethylene glycol. Urinary calcium, phosphate, oxalate, protein, uric acid, creatinine was reduced and urinary magnesium level was elevated. It also increased the urine volume, thereby reducing the tendency for crystallization. The histopathological studies confirmed the induction of lithiasis as microcrystal deposition was observed in section of kidney from animals treated with ethylene glycol. This was reduced, however, after treatment with the extract. These observations enable us to conclude that alcoholic extract of Rotula aquatica is effective against ethylene-glycol induced urolithiasis in albino rats.

  12. Effects of shading on Vallisneria natans (Lour.) H. Hara growth

    DEFF Research Database (Denmark)

    Fox, Anthony David; Meng, F; Shen, X;

    2013-01-01

    , but dry weight fruit production was significantly reduced at 90% shading. We conclude that above 50% surface shading, V. natans plants suffer reductions in accumulated biomass and investment in sexual reproduction. We contend that recent expansions in the extent of the native floating water chestnut...... Trapa spp. at seasonally inundated wetlands in the Yangtze River floodplain could, by shading, have contributed to the reduction in annual biomass and seed production of V. natans, contributing to declines in distribution and abundance....

  13. Evaluation of Physiochemical Screening and Standardization on the Root of Rotula aquatica Lour.

    Directory of Open Access Journals (Sweden)

    Abhishek B*, Avinash Saini

    2013-03-01

    Full Text Available The root of Rotula aquatica is also called as pashanbed, belonging to the family Borogenaceae. It is widely distributed in India from kumaun to Assam and western to southern India.The medicinal values of plant lie in their component phytochemicals such as alkaloids, flavonoids, phenolic compounds and other nutrients like as amino acid, proteins, which produce a definite physiological action on the human body. The proximate values such as moisture content, ash value, extractive value, were carried out. The dried roots powder was subjected to successive extraction with different solvents like petroleum ether, benzene, chloroform, methanol, ethanol and water. Macroscopic as well as microscopic studies of any crude drug are the primary steps to establish its botanical quality control before going to other studies. Hence Standardization of crude drug plays a very important role in identifying the purity and quality of crude drugs. The present investigation reveals Standardization which include Moisture Content, Total ash, Acid insoluble ash, Water soluble ash, Water soluble Extractive value, Alcohol soluble Extractive value, Phytochemical screening, and further isolation and identification of Phytoconstituents.

  14. A responsabilidade social na Câmara Municipal de Loures: projecto aplicado à Freguesia de Camarate

    OpenAIRE

    Ribeiro, Ana Cecília de Seabra Martins Saldanha

    2013-01-01

    Mestrado em Gestão responsabilidade social organizacional é um conceito cada vez mais, implícito na gestão organizacional. Uma organização é socialmente responsável quando tem em conta, nos seus próprios objectivos, o ambiente, a comunidade onde está inserida e o capital humano. Existe assim, um investimento no desenvolvimento económico, tecnológico, social, cultural, ambiental, tendo em conta os direitos humanos, os valores e princípios de uma sociedade com ét...

  15. Antioxidant and Anticancer Activities of Wampee (Clausena lansium (Lour. Skeels Peel

    Directory of Open Access Journals (Sweden)

    K. Nagendra Prasad

    2009-01-01

    Full Text Available Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT. In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901, human hepatocellular liver carcinoma (HepG-2 and human lung adenocarcinoma (A-549 cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

  16. Lactagogue effects of Torbangun, a Bataknese traditional cuisine.

    Science.gov (United States)

    Damanik, Rizal; Wahlqvist, Mark L; Wattanapenpaiboon, N

    2006-01-01

    Coleus amboinicus Lour (CA) has been used as a breast milk stimulant (a lactagogue) by Bataknese people in Indonesia for hundreds of years. However, the traditional use of CA is not well documented, and scientific evidence is limited to establish CA as a lactagogue. This investigation was conducted to elucidate the effect of traditional use of CA during the first month of lactation on quantity and quality of the breast milk. The results collected from the study show that CA supplementation increased breast milk production without compromising the nutritional quality of the breast milk. Lactating women receiving CA supplementation had a 65% increase in milk volume during the last two weeks of supplementation (from Day 14 to Day 28). This increase was greater than that of lactating women receiving Molocco+B12 tablets (10%) or Fenugreek seeds (20%). The residual effects of CA supplementation were seen even after the supplementation had ended for one month. Results of the present study confirmed the belief and the practice amongst the Bataknese people that CA can be used as a lactagogue in humans, and the use of CA might be suitable for lactating women in general.

  17. Using of Bangun-Bangun Leafs (Coleus Amboinicus, L on Red Sugar Block to Upgrading Milk Production of Frisian Holstein

    Directory of Open Access Journals (Sweden)

    Nelzi Fati

    2014-01-01

    Full Text Available Science and technology for the Community Activities Program (IBM granting wake leaf meal in urea red sugar  block has been implemented in a herd prosperous green valley village Kampung Manggis, Western District of Padang Panjang, Municipality of Leopold. This activity aims to increase the milk production of dairy cows through the application of technology utilization wake leaf meal in urea  red sugar block. The benefits of this activity is to assist farmers in improving milk production per day, so that milk production can increase the impact on increasing revenue. To achieve the goal of service to the community mentioned above, it has been conducted lectures, live demonstrations on the making lick candy made from leaf shapes. Six cows used as a demonstration, two dairy cows with urea red sugar block containing 2.5% leaf shapes, two dairy cows with urea red sugar block containing leaf shapes 5% and two cows with urea red sugar block containing leaves wake up 7.5%. Wake up dried leaves with the help of sunlight and then made ready for use in the flour mixture forming urea red sugar block which serves as a feed supplement in dairy cattle. Evaluation activities indicate that the application of urea  red sugar block starchy wake leaves 5% can increase the production of milk 2 liters / day / head (average increase 10%.Of this service activities can be concluded that the use of leaf meal wake up in 5% urea red sugar  block can increase milk production of dairy cows 2 liters / day / head compared with 2.5%, 7.5%.

  18. Adjuvant effect of an extract from Cochinchina momordica seeds on the immune responses to ovalbumin in mice

    Institute of Scientific and Technical Information of China (English)

    XIAO Chenwen; HU Songhua; Rajput Zahid Iqbal

    2007-01-01

    The seed of the Momordica cochinchinensis (Lour.)Spreng as a traditional Chinese medicine has been utilized in China for more than 1 200 years.It is traditionally used for the treatment of inflammatory swelling,scrofula,tinea,diarrhea as well as suppurative skin infections such as sore,carbuncles,furuncles and boils in both humans and animals.In this study,an extract from Cochinchina momordica seeds(ECMS)is evaluated for its adjuvant effect and safety.The results suggest that when co-administered with ECMS in Balb/c mice,ovalbumin(OVA,10μg)may induce significantly higher specific antibody production than OVA used alone(P<0.05).Analysis of antibody isotypes indicates that the ECMS can promote the production of both IgG1 and IgG2a,concanavalin A,lipopolysaccharides or OVA are significantly higher in mice immunized with OVA mixed with ECMS than immunized with OVA alone or mixed with aluminum hydroxide(P<0.05).No local reactions and negative effects on the body weight gain occurred after the injection of OVA mixed with various amounts of ECMS in mice.Therefore,the ECMS is safe for injection and can be used as a potential vaccine adjuvant biasing the production of IgG2a in mice.

  19. Enraizamento e germinação na propagação de Pfaffia glomerata (Spreng. Pedersen (ginseng-brasileiro Rooting and germination in propagation of Pfaffia glomerata (Spreng. Pedersen (ginseng-brasileiro

    Directory of Open Access Journals (Sweden)

    Silvana de Paula Quintão Scalon

    2009-10-01

    Full Text Available Conduziu-se este trabalho, com o objetivo de avaliar a propagação sexuada e assexuada de Pfaffia glomerata (ginseng brasileiro. As sementes foram pré-embebidas por 24 horas em KNO3 1%; ácido giberélico 50, 100 e 200 mg L-1 ; emágua e as sementes que não receberam tratamento serviram como testemunha. A semeadura foi em placas de petri sobre duas folhas de papel de filtro e a incubação a 25ºC, sob iluminação constante e na ausência de luz. As estacas com º15 cm foram imersas durante 6 horas em IAA 250 e IBA 500 mg L-1, ácido bórico 1% e em água e plantadas em embalagem plástica, contendo terra+areia (1:1. O primeiro experimento foi conduzido em delineamento inteiramente casualizado em esquema fatorial 2 (níveis de luz x 6 (tratamento de pré-embebição em 4 repetições de 20 sementes. O segundo em DIC com 5 tratamentos, 4 repetições de 15 estacas por repetição. As sementes de ginseng brasileiro necessitam de tratamento de pré-embebição para acelerar a germinação, sendo a imersão em KNO3 o método mais eficiente, proporcionando 63% de germinação. Apresentam fotoblastismo neutro, com germinação média de 45%. As estacas de ginseng brasileiro imersas apenas em água apresentaram 100% de enraizamento, maior número de brotos (2,5 e maior comprimento de raiz (8,6 cm. A espécie apresenta potencial para ambos os métodos de propagação.The objective of this study was to evaluate sexual and asexual propagation of Pfaffia glomerata (Brazilian Ginseng. The seeds were pre-soaked for 24 hours in KNO3 1% , gibberellic acid 50, 100, 200 mg L-1 , and in water. Untreated seeds served as control. Sowing was accomplished in petri dishes on 2 sheets of filter paper, at 25ºC incubation, under constant illumination, and in the absence of light. The cuttings with º1,5 cm were immersed for 6 hours in IAA 250 and 500mg L-1, boric acid1% ,and in water. Soon after they were planted in plastic containers with soil + sand (1:1. The first experiment was in factorial design 2 (light levels x 6 (pre-soak treatment in 4 repetitions of 20 seeds per repetition. The second in ERD in 5 treatments, 4 repetitions of 15 cuttings per repetition. Brazilian Ginseng seeds need pre-soak treatment to accelerate germination. We found immersion in KNO3 the most efficient method, providing 63% germination. They presented neuter photoblastism with an average germination of 45%. The Brazilian Ginseng cuttings immersed in water presented 100% rooting, a higher number of sprouts (2,5, and greater length (8,6 cm. The species presents potential for both propagation methods.

  20. Morfologia do fruto, da semente e morfo-anatomia da plântula de Vitex megapotamica (Spreng. Moldenke (Lamiaceae Fruit and seed morphology and morpho-anatomy of seedlings of Vitex megapotamica (Spreng. Moldenke (Lamiaceae

    Directory of Open Access Journals (Sweden)

    Nelson Luiz Cosmo

    2009-06-01

    Full Text Available Vitex megapotamica (tarumã é espécie arbórea, decídua, com ocorrência, no Brasil, desde Minas Gerais até o Rio Grande do Sul. Visando à caracterização morfológica do fruto, da semente e morfo-anatômica da plântula, frutos desta espécie foram coletados e as sementes postas para germinar em laboratório. As plântulas foram coletadas desde a protrusão da raiz até o desenvolvimento do primeiro par de eofilo. Foram realizadas medições e pesagem de frutos, e contagem do número de sementes por frutos. As características morfológicas do fruto e da semente são aqui descritas e ilustradas, assim como a morfo-anatomia da plântula. O fruto é drupóide, nuculânio, tetralocular, contendo apenas uma ou duas sementes com fina camada de endosperma e embrião axial, foliáceo. O diásporo (pirênio é constituído pelo endocarpo mais a semente. O endocarpo lenhoso parece exercer restrição sobre a germinação das sementes desta espécie. A plântula é epigea, fanerocotiledonar, com paracotilédones elípticos, com margem inteira, e eofilos opostos, simples, elípticos, com margem serreada, apresentando tricomas tectores. Tanto o paracotilédone quanto o eofilo apresentam mesofilo heterogêneo, dorsiventral, feixe colateral em forma de arco e estômatos anomocíticos. A raiz é poliarca, com córtex parênquimático; o hipocótilo possui tricomas glandulares e não-glandulares, colo distinto, e com cerca de 20 dias encontra-se em início de crescimento secundário. Diversas das características da plântula de Vitex megapotamica estão relacionadas à sua condição de espécie heliófila.Vitex Megapotamica (tarumã is a deciduous tree occurring in Brazil from Minas Gerais to Rio Grande do Sul States. In order to characterize fruit and seed morphology and morpho-anatomy of seedlings, fruits of this species were collected and seeds were germinated in the laboratory. Seedlings were collected from root protrusion to development of the first pair of eophylls. The fruits were measured and weighed and the number of seeds per fruit counted. Fruit and seed morphology were described and illustrated, as well as morphoanatomy of the seedling. The fruit is a 4-locular drupe containing only one or two seeds with a thin layer of endosperm and an axial, foliate embryo. The woody endocarp seems to restrict germination in this species. The seedling has epigeal, phanerocotylar development; elliptical paracotyledons with entire margins, and opposite, simple, elliptical eophylls with serrate margins, and simple multicelullar trichomes. Paracotyledons and eophylls have heterogeneous dorsiventral mesophyll, collateral bundle, and anomocytic stomata. The root is polyarc, with parenchymatous cortex; the hypocotyl has glandular and non-glandular trichomes, a distinct collet, and within 20 days it starts secondary growth. Many characteristics of seedlings in V. megapotamica are related to the fact that it is a heliophyte.

  1. Morfoanatomia e ontogênese do fruto e semente de Vernonia platensis (Spreng. Less. (Asteraceae Morphology, anatomy and ontogeny of the fruit and seed of Vernonia platensis (Spreng. Less. (Asteraceae

    Directory of Open Access Journals (Sweden)

    Natália Arias Galastri

    2010-03-01

    Full Text Available Asteraceae possui cerca de 23.000 espécies e Vernonieae tem sua maior representatividade no Brasil, sendo Vernonia o maior gênero da tribo. Devido à ampla ocorrência nos Cerrados, V. platensis foi selecionada para a realização deste trabalho, que objetiva descrever a morfoanatomia e o desenvolvimento do pericarpo e da semente desta espécie, comparando os resultados com a literatura. O material coletado foi processado segundo técnicas usuais. O ovário é ínfero, bicarpelar, sincárpico, unilocular, com um óvulo anátropo, unitegumentado, tenuinucelado, formado em placentação basal. A parede ovariana é homogênea, com células mais densas perifericamente. O tegumento possui três regiões, destacando-se evidente endotélio. Na maturidade, a maioria das camadas colapsa, mantendo-se as fibras mesocárpicas externas; o pápus duplo persiste formado por células lignificadas. A semente madura apresenta testa restrita a uma faixa de células colapsadas; o endosperma é celular, persistindo residualmente na maturidade, e o embrião exibe eixo hipocótilo-radícula axial, espesso e curto. Em apenas 40% das cipselas maduras analisadas, há sementes completamente desenvolvidas. As observações deste trabalho corroboram pesquisas anteriores com Asteraceae, mas destaca-se o papel nutritivo do tegumento no desenvolvimento seminal e a baixa produção de sementes em V. platensis.The Asteraceae comprises approximately 23,000 species and Vernonieae is best represented in Brazil; Vernonia is the largest genus in this tribe. Due to occurrence in extensive areas of Cerrado, Vernonia platensis was selected for this work that aims to describe the morphology, anatomy and development of the pericarp and seed of this species, comparing the results with the literature. The collected material was processed by conventional techniques. The ovary is inferior, bicarpellate, syncarpous, unilocular with a single anatropous ovule, unitegmic, tenuinucelate, attached in a basal placenta. The ovary wall is homogenous with dense cells peripherally. The integument has three zones, with a conspicuous endothelium. At maturity, most layers collapse, only the outer mesocarp fibers remaining; the double pappus persists and has lignified cells. The mature seed has a testa composed of collapsed cells; the endosperm is cellular, residual at maturity, and the embryo has a thick, short, and axial embryo axis. Completely developed seeds were found in only 40% of the mature cypselae evaluated. Our observations corroborate previous research with Asteraceae; we call attention to the nutritive role of the seed coat in seed development, and the low production of seeds in V. platensis.

  2. Observações sobre o modo de reprodução em Adesmia latifolia Spreng. Vog. Observations on the mode of reproduction of Adesmia latifolia Spreng. Vog.

    Directory of Open Access Journals (Sweden)

    Solange Bosio Tedesco

    1998-03-01

    Full Text Available Um experimento foi conduzido em casa de vegetação, com plantas de um acesso de Adesmia latifolia, para fazer observações relativas ao seu modo de reprodução. As plantas foram isoladas individualmente e, após o florescimento, foram submetidas a três tratamentos: estímulo mecânico, cruzamentos manuais e isolamento. Os resultados indicaram versatilidade da espécie, a qual pode reproduzir-se por fecundação cruzada e por autofecundação.An experiment was carried out to study the reproduction mode of Adesmia latifolia. The experiment was conducted in greenhouse with plants of one access of this species. The plants after flowering were isolated individually and submitted to three treatments: mechanical stimulation, hand crossing and isolation. The results showed versatility of this species which could reproduce by cross-fertilization and self-fertilization.

  3. 香港大榄郊野公园的植物组成与植被数量分类%Floristic Composition and Quantitative Classification in Tai Lam Country Park,Hong Kong

    Institute of Scientific and Technical Information of China (English)

    曾绮微; 李海生; 陈桂珠; 叶创兴; 黄颂诗; 黄超弘

    2008-01-01

    通过对香港大榄郊野公园森林群落的样地调查,利用组平均法和除趋势对应分析两种方法,对样方进行聚类与排序分析.把35个样地划分为11个植被类型,森林群落可划分为台湾相思(Acacia confusa Merr.)林、红胶木(Lophostemon confertus (R. Br.) Peter G. Wilson ex J. T. Waterh.)林、木荷(Schima superba Gardner ex Champ.)林、柠檬桉(Eucalyptus citriodora Hook.)林、湿地松+鸭脚木(Pinus elliottii Engelm.+ Schefflera octophylla (Lour.) Harms.)林、土沉香(Aquilaria sinensis (Lour.) Spreng.)林、毛叶桉(Eucalyptus torelliana F. Muell.)林; 灌木群落分为山油柑+越南叶下珠(Acronychia pedunculata (L.) Miq.+ Phyllanthus cochinchinensis (Lour.) Spreng.)矮树灌丛、豺皮樟+桃金娘(Litsea rotoundifolia (Nees) Hemsl. var. oblongifolia (Nees) C. K. Allen+Rhodomyrtus tomentosa (Alton) Hassk.)矮树灌丛、大头茶(Gordonia axillaris (Roxb. ex Ker Gawl.) D. Dietr.)矮树灌丛、桃金娘+芒萁(Rhodomyrtus tomentosa (Alton) Hassk.+Dicranopteris dichotoma (Thunb.) Bernh.)灌草丛.DCA排序结果反映海拔高度与各个森林群落的分布格局和物种组成关系不大,而环境湿度是影响森林群落物种组成的主要因素.多样性分析结果显示,台湾相思林和土沉香林多样性指数最高,说明台湾相思林逐渐向次生林演变,除了红胶木林和少数郁闭度低的台湾相思林中有强阳生性树种外,大部分人工林下植物以阳生性稍耐阴的树种为主.调查结果显示,大榄郊野公园植物生长条件东部优于西部,西部的物种丰富度和多样性指数较低,主要以人工林和灌丛为主,加上山火频繁发生,导致长期处于先锋阶段.

  4. Human perceptions of landscape change: The case of a monodominant forest of Attalea speciosa Mart ex. Spreng (Northeast Brazil).

    Science.gov (United States)

    de Almeida, Gabriela M A; Ramos, Marcelo A; Araújo, Elcida L; Baldauf, Cristina; Albuquerque, Ulysses Paulino

    2016-05-01

    From the perception of human populations, we can assess the changes occurring in certain landscapes and the factors that cause those changes. Such studies have proven helpful in increasing the knowledge of the history of a landscape, recognizing past formations and projecting its future. Our research objective was to determine how a landscape dominated by the palm tree Attalea speciosa, a species of ecological, economic, and cultural importance, has been changing over time by synthesizing and comparing historical documents and local perceptions. This study was conducted in Araripe Environmental Protection Area, Northeast Region, Brazil. To understand local landscape change, we interviewed active harvesters in four communities in which A. speciosa use has been documented. Historical documents were evaluated as a complement to the interview data. According to local informants, areas previously used for cultivation and animal husbandry that were abandoned or decimated by droughts in the region may have fostered the expansion of a monodominant A. speciosa forest. Furthermore, other forms of landscape management resulting from human population growth may also have affected the current and past distribution of this forest.

  5. Comparative Study of the Leaf Volatiles of Arctostaphylos uva-ursi (L. Spreng. and Vaccinium vitis-idaea L. (Ericaceae

    Directory of Open Access Journals (Sweden)

    Radosav Palić

    2010-09-01

    Full Text Available The first GC and GC/MS analyses of the essential oils hydrodistilled from dry leaves of Arctostaphylos uva-ursi and Vaccinium vitis-idaea enabled the identification of 338 components in total (90.4 and 91.7% of the total GC peak areas, respectively. Terpenoids, fatty acids, fatty acid- and carotenoid derived compounds were predominant in the two samples. Both oils were characterized by high relative percentages of α-terpineol and linalool (4.7-17.0%. Compositional data on the volatiles of the presently analyzed and some other Ericaceae taxa (literature data were mutually compared by means of multivariate statistical analyses (agglomerative hierarchical cluster analysis and principal component analysis. This was done in order to determine, based on the essential oil profiles, possible mutual relationships of the taxa within the family, especially that of species from the genera Arctostaphylos and Vaccinium. Results of the chemical and statistical analyses pointed to a strong relation between the genera Vaccinium and Arctostaphylos.

  6. LOMBRICOMPOSTAS Y APERTURA DE LA ESPATA EN POSCOSECHA DEL ALCATRAZ "GREEN GODDESS" (Zantedeschia aethiopica (L) K. Spreng) EN CONDICIONES TROPICALES

    OpenAIRE

    J. G. Cruz-Castillo; P. A. Torres-Lima; M. Alfaro-Chilmalhua; M. L. Albores-González; J. Murguía-González

    2008-01-01

    En un área tropical de altura se llevaron a cabo estudios sobre el alcatraz Green Goddess . Las plantas, bajo un sombreado de 35 %, fueron colocadas en macetas con varios tipos de lombricomposta; pulpa de café + estiércol de bovino; pulpa de café + gallinaza + bagazo de caña. Otros tratamientos fueron pulpa de café + fertilizante químico (12-11-18-3 mg) + Organozyma® (promotor natural de crecimiento). El área foliar, el número de tallos emergidos y la longitud del escapo floral fueron signif...

  7. Effect of benzyladenine on the abundance and quality of the leaf yield in the calla lily (Zantedeschia Spreng.

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    Beata Janowska

    2013-03-01

    Full Text Available The soaking of rhizomes, 15-18 cm in circumference in the cultivars ‘Black Magic’ and ‘Mango’ and 20+ cm in cultivar ‘Albomaculata’, with leaf buds 0.5-2 cm in length, in solutions of benzyladenine at concentrations of 100, 350 and 600 mg·dm-3 lasted 30 and 60 min. After this practice, lightly dried rhizomes were planted into 20-cm pots. BA at concentrations of 100-600 mg·dm-3 reduces the yield of leaves in cultivar ‘Mango’, and at concentrations of 350-600 mg·dm-3 – in cultivar ‘Albomaculata’. Leaves growing from rhizomes soaked in BA at concentrations of 100-600 mg·dm-3 have a higher greenness index and a higher protein and sugar content.

  8. Effect of flurprimidol and benzyladenine on growth and flowering of calla lily (Zantedeschia Spreng. cultivated in pots

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    Beata Janowska

    2011-03-01

    Full Text Available Effect of flurprimidol used directly into root and benzyladenine used for rhizomes soaking on growth and flowering of two Zantedeschia cultivars was evaluated: ‘Pink Pimpernel and ‘Mango’. Plants bloomed more abundantly after application of benzyladenine and flurprimidol in compare with control plants. The greatest number of flowers was noticed after rhizomes soaking in solution of benzyladenine connected with plants watering solution of flurprimidol in concentration of 112.5 mg·dm-3. Flurprimidol and also benzyladenine inhibited growth of peduncles, but had no effect on length of spathes. The greater number of leaves was noticed after application of benzyladenine in ‘Pink Pimpernel’ cultivar, except combination with benzyladenine in concentration of 100 mg·dm-3 and flurprimidol in concentration of 75.0 mg·dm-3. Cultivar ‘Pink Pimpernel’ had shorter petioles, more rounded leaf blades and higher leaf greenness index in response of benzyladenine and benzyladenine with retardant. Flurprimidol had stimulating effect on the number of leaves and benzyladenine inhibited this feature in ‘Mango’ cultivar. More rounded leaf blades were noticed only in plants of ‘Mango’ cultivar treated with retardant in concentration of 112.5 mg·dm-3.  

  9. Anesthesia and transport of fat snook Centropomus parallelus with the essential oil of Nectandra megapotamica(Spreng. Mez

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    Juliana Simoni Moraes Tondolo

    2013-09-01

    Full Text Available This study analyzed the chemical composition and anesthetic potential of essential oil (EO of Nectandra megapotamica in fat snook (Centropomus parallelus. For the extraction of EO by hydrodistillation, leaves were separated in young (EO-Y or old (EO-O, and the chemical composition of the EOs was determined by CG-MS. The anesthetic potential was assessed by the evaluation of induction and recovery time of anesthesia and stress response from anesthesia and transport. Three experiments were carried out: i four different concentrations of each EO were tested to evaluate anesthesia induction and recovery time; ii two concentrations of EO-O were tested for the evaluation of its effects on stress parameters (glucose, lactate, and Na+ and K+ plasma levels caused by anesthesia; and iii fish were transported in plastic bags, supplied with two concentrations of EO-O for the evaluation of water quality and mortality. All experiments were performed on fish acclimated to 0 and 33 ppt salinity. The main constituents of the Y and O-EOs were bicyclogermacrene (46.5/34.6%, α-pinene (26.8/26.2%, β-pinene (7.9/12.3%, and germacrene D (9.6/9.1%. Mild sedation was achieved at 30 °L L-1(1.3-3.2 min and deep anesthesia at 150 °L L-1(5.6-8.0 min with both EOs. The recovery time ranged from 1-10 min. The EO-O was not able to avoid the stress of anesthesia evidenced by elevated glucose and lactate plasma levels observed in all groups. Plasma levels of Na+ and K+ were not significantly affected by treatments. During transport, the use of EO-O did not prevent deterioration in water quality and the post-transport mortality. In conclusion, the EO of N. megapotamica has anesthetic activity in fat snook, but it was not able to prevent the stress of anesthesia and transport.

  10. Chemical Composition and Physical Characteristics of the Essential Oil of Cymbopogon schoenanthus (L.) Spreng of Burkina Faso

    Science.gov (United States)

    Yentema, Onadja; Alioune, Ouedraogo; Abdoul Dorosso, Samate

    Essential oils have a significant role in the society where they are variously used in fields such as medicine, pharmacy, cosmetics, chemical and food-processing industries. This justifies interest which they cause and thus systematic studies are undertaken on aromatic species, allowing without any doubt their use advisedly. This study concerns the characterization of the essential oil of Cymbopogon schoenanthus of Burkina Faso, extracted by drive with the water vapor in a distillation operation. The analysis of the chemical composition is carried out by Gas Chromatography. It shows that the 16 made up ones identified account for 65. 2% of the essential oil composition. These compounds belong to the two classes regularly met in essential oils: the mono ones and sesquiterpenes. However, proportion of monoterpenes (53. 2%) is higher than that of sesquiterpenes (12%). Among the identified compounds two monoterpenes (the piperitone and δ-2-carene) remain the principal components in the essential oil. Then the authors determine the density d = 0.9057 by double weighing, the optical activity α = +28.175 by polarimetry and the refractive index n = 1.465 by an interferometric method which they describe. Cymbopogon schoenanthus is an aromatic plant of the family of Poaceae very often used in traditional pharmacopoeia for internal as well as external treatments.

  11. The silk characteristics of jedung silkworm (Attacus atlas L. reared on senggugu (Clerodendron serratum Spreng food plant

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    MUHAMMAD INDRAWAN

    2007-07-01

    Full Text Available The objective of this research was to determine the silk characteristics of Jedung silkworm reared on Senggugu food plant. Cocoon characteristics examined was the weight of cocoon and the value of Shell Ratio (SR. Meanwhile, the characteristics of fiber conclude the tensile strength, the elongation and the moisture absorption. The results showed that the range of cocoon weight was between 5.849 + 0.378 g. If it is compared to other cocoon reared on other food plants, the cocoons from Jedung silkworm were less weight. This was probably because of the characteristics of Senggugu leaf that was thinner and less water content which will produced cocoon with less weight. The lower weight of cocoon could affect to the value of measured SR. The value of SR was 9.828 + 1.475 %. The good range of SR value is between 19-20 %. The tensile strength was 14,444 + 5.270 g per denier. The good range of tensile strength is between 2.6 to 4.8 g per denier. The elongation was 2.184 + 2.168 %. This study showed the elongation was lower compared to other characteristics of good fiber with value more than 18 %. The moisture absorption varies between 20.214 + 0.618 %. It will cause fabric made from this material were considered cooler to wear, so that relatively good from the side of convenience.In general, characteristics of the fiber were relatively good. Therefore, it needs higher effort to increase weight of cocoon and the elongation of fiber.

  12. The silk characteristics of jedung silkworm (Attacus atlas L.) reared on senggugu (Clerodendron serratum Spreng) food plant

    OpenAIRE

    MUHAMMAD INDRAWAN

    2007-01-01

    The objective of this research was to determine the silk characteristics of Jedung silkworm reared on Senggugu food plant. Cocoon characteristics examined was the weight of cocoon and the value of Shell Ratio (SR). Meanwhile, the characteristics of fiber conclude the tensile strength, the elongation and the moisture absorption. The results showed that the range of cocoon weight was between 5.849 + 0.378 g. If it is compared to other cocoon reared on other food plants, the cocoons from Jedung ...

  13. Chemical constituents of Galianthe brasiliensis (Spreng.) E.L.Cabral and Bacigalupo (Rubiaceae); Constituintes quimicos de Galianthe brasiliensis (Rubiaceae)

    Energy Technology Data Exchange (ETDEWEB)

    Moura, Wagner Marques de; Santos, Daniela Pereira dos; Santini, Silvana Maria de Oliveira [Universidade Estadual de Maringa, PR (Brazil). Dept. de Quimica]. E-mail: smoliveira@uem.br; Carvalho, Joao Ernesto de; Foglio, Mary Ann [Centro Pluridisciplinar de Pesquisas Quimicas, Biologicas e Agricolas, Campinas, SP (Brazil)

    2006-05-15

    The paper describes the chemical constituents isolated from aerial parts of the plant Galianthe brasiliensis. From a methanol extract, the iridoid glycosides asperuloside, deacetylasperuloside, mixture of Z- and E-6-O-p-coumaroylscandoside methyl ester, the triterpene ursolic acid and the steroids stigmasterol, campesterol, b-sitosterol and 3-O-b-glucopyranosyl sitosterol were isolated. The structures of the natural products were identified on the basis of spectral data, including 2D NMR experiments. The antiproliferative properties of the crude methanolic extract were investigated against a series of nine human cancer cell lines. (author)

  14. Morphological, physico-chemical and structural characterization of mucilage isolated from the seeds of Buchanania lanzan Spreng

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    Sudarshan Singh

    2014-01-01

    Full Text Available Context: Mucilage isolated from the seeds of Buchanania lanzan a wild Indian plant. This mucilage can be commercially exploited, by evaluating physico-chemical properties of this mucilage. Materials and Methods: Various physico-chemical parameter using scanning electron microscopy (SEM, molecular weight, X-ray diffraction (XRD spectrometry, zeta potential (ZP, Fourier transform infrared (FT-IR spectroscopy and 1D ( 1 H and 13 C nuclear magnetic resonance (NMR have been employed to characterize mucilage in the present study. Results: SEM analysis suggests that the mucilage has irregular particle size. Thermogravimetry analysis suggested that mucilage had good thermal stability with two stage decomposition. The weight-average molecular weight of mucilage was determined to be 4883, by gel permeation chromatography. The XRD pattern of the mucilage indicated a completely amorphous structure. The ZP was obtained 1.56 and −9.49 mV in water and 0.1 N NaCl respectively. The major functional groups identified from FT-IR spectrum include 3459/cm (-OH, 1667/cm (Alkenyl C-H and C = C stretch, 1407/cm (-COO- and 1321/cm (-CH 3 CO. Analysis of mucilage by paper chromatography and 1D NMR indicated the presence of rhamnose, arabinose and fructose. Conclusion: The spectral and chromatography analysis of mucilage indicate that the mucilage is composed of basic sugar moiety such as (arabinose, rhamnose, fructose and mannose as complex carbohydrates.

  15. TOLERANCE OF CANAFÍSTULA (Peltophorum dubium (SPRENG TAUB. SEEDLINGS INOCCULATED WITH Pisolithus microcarpus TO COPPER CONTAMINATED SOIL

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    Rodrigo Ferreira da Silva

    2010-08-01

    Full Text Available The ectomycorrhizal fungi symbiosis with native florestal essences may be an alternative to   revegetation of metal contaminated soils. The aim of this work was to determine canafístula seedlings behavior, inoculated to ectomycorrhizal fungi in soil with copper excess. The trial was conducted in greenhouse conditions. The ectomycorrhizal fungi used was Pisolithus microcarpus UFSC Pt116, cultivated on peat-vermiculite substrate (3:1. The canafístula seedlings were developed in washed  sand and transplanted, when showed two definitive leaves. Vase of 1L with 1 kg of soil were used as experimental unit. The inoculation was accomplished in the seedlings transplant, added 2g of inoculates for experimental unit. The experimental design was entirely casual in bi factorial scheme (2 x 4 qualitative in A, with and without fungi inoculum and quantitative in D being the control addition of 150 mg Kg -1, 300 mg Kg -1 and 450 mg Kg -1 of copper (CuSO4, with seven repetitions. Plant height, colon diameter, dry matter, copper content, mycorrhizal colonization percentage, superficial roots area and roots length were assessed. The increase of soil copper levels reduced roots length, specific superficial roots area, height and plant diameter of canafístula. Canafistula seedlings inoculated to ectomycorrhizal fungi showed low cooper amounts in their tissue and copper absortion reduced in high copper doses.

  16. Emission and Accumulation of Monoterpene and the Key Terpene Synthase (TPS) Associated with Monoterpene Biosynthesis in Osmanthus fragrans Lour

    OpenAIRE

    Zeng, Xiangling; Liu, Cai; Zheng, Riru; Cai, Xuan; Luo, Jing; Zou, Jingjing; Wang, Caiyun

    2016-01-01

    Osmanthus fragrans is an ornamental and economically important plant known for its magnificent aroma, and the most important aroma-active compounds in flowers are monoterpenes, mainly β-ocimene, linalool and linalool derivatives. To understand the molecular mechanism of monoterpene production, we analyzed the emission and accumulation patterns of these compounds and the transcript levels of the genes involved in their biosynthesis in two O. fragrans cultivars during flowering stages. The resu...

  17. Emission and accumulation of monoterpene and the key terpene synthase (TPS associated with monoterpene biosynthesis in Osmanthus fragrans Lour.

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    Xaingling eZeng

    2016-01-01

    Full Text Available Osmanthus fragrans is an ornamental and economically important plant known for its magnificent aroma, and the most important aroma-active compounds in flowers are monoterpenes, mainly β-ocimene, linalool and linalool derivatives. To understand the molecular mechanism of monoterpene production, we analyzed the emission and accumulation patterns of these compounds and the transcript levels of the genes involved in their biosynthesis in two O. fragrans cultivars during flowering stages. The results showed that both emission and accumulation of monoterpenes varied with flower development and glycosylation had an important impact on floral linalool emission during this process. Gene expression demonstrated that the transcript levels of terpene synthase (TPS genes probably played a key role in monoterpene production, compared to the genes in the MEP pathway. Phylogenetic analysis showed that OfTPS1 and OfTPS2 belonged to a TPS-g subfamily, and OfTPS3 and OfTPS4 clustered into a TPS-b subfamily. Their transient and stable expression in tobacco leaves suggested that OfTPS1 and OfTPS2 exclusively produced β-linalool, and trans-β-ocimene was the sole product from OfTPS3, while OfTPS4, a predictive sesquiterpene synthase, produced α-farnesene. These results indicate that OfTPS1, OfTPS2 and OfTPS3 could account for the major floral monoterpenes, linalool and trans-β-ocimene, produced in O. fragrans flowers.

  18. Emission and accumulation of monoterpene and the key terpene synthase (TPS) associated with monoterpene biosynthesis in Osmanthus fragrans Lour.

    OpenAIRE

    Xaingling eZeng; Cai eLiu; Riru eZheng; Xuan eCai; Jing eLuo; Jingjing eZou; Caiyun eWang

    2016-01-01

    Osmanthus fragrans is an ornamental and economically important plant known for its magnificent aroma, and the most important aroma-active compounds in flowers are monoterpenes, mainly β-ocimene, linalool and linalool derivatives. To understand the molecular mechanism of monoterpene production, we analyzed the emission and accumulation patterns of these compounds and the transcript levels of the genes involved in their biosynthesis in two O. fragrans cultivars during flowering stages. The resu...

  19. Kinnow madarin (Citrus nobilis lour × Citrus deliciosa tenora fruit waste silage as potential feed for small ruminants

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    B. A. Malla

    2015-01-01

    Full Text Available Aim: Study was conducted to ascertain the quality of Kinnow mandarin waste (KMW silage and its utilization by adult male goats. Materials and Methods: KMW was collected, dried to 30% dry matter level and ensiled in silo pit after addition of disodium hydrogen orthophosphate as source of phosphorus as KMW is deficient in phosphorus. Oat was collected at milking stage, chopped finely and ensiled in a silo pit for 2 months. Twelve nondescript local adult male goats of about 8-10 months age and mean body weight of 23.00±0.90 kg were selected. The goats were randomly allotted on body weight as per randomized block design into two equal groups, six animals in each group (n=6 namely “oat silage (OS” and “Kinnow silage.” Goats were offered weighed quantities of respective silage on ad libitum basis. The silages were evaluated for proximate principles and silage quality attributes. Results: Differences were found between chemical composition of both silages with higher organic matter, ether extracts, nitrogen free extract (p0.05 for CP and possess comparable (2.23 vs. 2.06; p>0.05 calcium content. The pH, ammonia nitrogen (percent of total nitrogen and soluble carbohydrate content were lower (4.20 vs. 3.30; 4.14 vs. 3.80; 2.73 vs. 1.86; p0.05 among the two dietary groups. Conclusion: It can be concluded that KMW can be used to prepare good quality silage for feeding of goats.

  20. Kinnow madarin (Citrus nobilis lour × Citrus deliciosa tenora) fruit waste silage as potential feed for small ruminants

    Science.gov (United States)

    Malla, B. A.; Rastogi, A.; Sharma, R. K.; Ishfaq, A.; Farooq, and J.

    2015-01-01

    Aim: Study was conducted to ascertain the quality of Kinnow mandarin waste (KMW) silage and its utilization by adult male goats. Materials and Methods: KMW was collected, dried to 30% dry matter level and ensiled in silo pit after addition of disodium hydrogen orthophosphate as source of phosphorus as KMW is deficient in phosphorus. Oat was collected at milking stage, chopped finely and ensiled in a silo pit for 2 months. Twelve nondescript local adult male goats of about 8-10 months age and mean body weight of 23.00±0.90 kg were selected. The goats were randomly allotted on body weight as per randomized block design into two equal groups, six animals in each group (n=6) namely “oat silage (OS)” and “Kinnow silage.” Goats were offered weighed quantities of respective silage on ad libitum basis. The silages were evaluated for proximate principles and silage quality attributes. Results: Differences were found between chemical composition of both silages with higher organic matter, ether extracts, nitrogen free extract (p0.05 for CP) and possess comparable (2.23 vs. 2.06; p>0.05) calcium content. The pH, ammonia nitrogen (percent of total nitrogen) and soluble carbohydrate content were lower (4.20 vs. 3.30; 4.14 vs. 3.80; 2.73 vs. 1.86; p0.05) among the two dietary groups. Conclusion: It can be concluded that KMW can be used to prepare good quality silage for feeding of goats. PMID:27046989

  1. Cloning, expression and characterization of COI1 gene (AsCOI1 from Aquilaria sinensis (Lour. Gilg

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    Yongcui Liao

    2015-09-01

    Full Text Available Aquilaria sinensis, a kind of typically wounding-induced medicinal plant with a great economical value, is widely used in the production of traditional Chinese medicine, perfume and incense. Coronatine-insensitive protein 1 (COI1 acts as a receptor in jasmonate (JA signaling pathway, and regulates the expression of JA-responsive genes in plant defense. However, little is known about the COI1 gene in A. sinensis. Here, based on the transcriptome data, a full-length cDNA sequence of COI1 (termed as AsCOI1 was firstly cloned by RT–PCR and rapid-amplification of cDNA ends (RACE strategies. AsCOI1 is 2330 bp in length (GenBank accession No. KM189194, and contains a complete open frame (ORF of 1839 bp. The deduced protein was composed of 612 amino acids, with a predicted molecular weight of 68.93 kDa and an isoelectric point of 6.56, and was predicted to possess F-box and LRRs domains. Combining bioinformatics prediction with subcellular localization experiment analysis, AsCOI1 was appeared to locate in nucleus. AsCOI1 gene was highly expressed in roots and stems, the major organs of agarwood formation. Methyl jasmonate (MeJA, mechanical wounding and heat stress could significantly induce the expression level of AsCOI1 gene. AsCOI1 is an early wound-responsive gene, and it likely plays some role in agarwood formation.

  2. Varietal classification and antioxidant activity prediction of Osmanthus fragrans Lour. flowers using UPLC-PDA/QTOF-MS and multivariable analysis.

    Science.gov (United States)

    Zhou, Fei; Peng, Jiyu; Zhao, Yajing; Huang, Weisu; Jiang, Yirong; Li, Maiquan; Wu, Xiaodan; Lu, Baiyi

    2017-02-15

    This study was aimed to classify the varieties and predict the antioxidant activity of Osmanthus fragrans flowers by UPLC-PDA/QTOF-MS and multivariable analysis. The PLS-DA model successfully classified the four varieties based on both the 21 identified compounds and the effective compounds. For the antioxidant activity prediction, PLS performed well to predict the antioxidant activity of O. fragrans flowers. Furthermore, acteoside, suspensaside A, ligustroside, forsythoside A, phillygenin and caffeic acid were selected as effective compounds by UVE-SPA for prediction. On the basis of effective compounds, PLS, MLR and PCR were applied to establish the calibration models. The UVE-SPA-MLR model was the optimal method to predict the antioxidant activity values with Rp of 0.9200, 0.9010 and 0.8905 for DPPH, ABTS and FRAP assays, respectively. The results revealed that the UPLC-PDA/QTOF-MS combined with chemometrics could be a new method to classify the varieties and predict the antioxidant activity of O. fragrans flowers. PMID:27664663

  3. Microcystin-RR uptake and its effects on the growth of submerged macrophyte Vallisneria natans (lour.) hara.

    Science.gov (United States)

    Yin, Liyan; Huang, Jiaquan; Li, Dunhai; Liu, Yongding

    2005-06-01

    Microcystins are hepatotoxins produced by many species of several cyanobacterial genera. Their toxic effects on animals and some terrestrial higher plants have been well studied, but their potential effects on the development of aquatic plant seedlings are not well known, and their uptake by aquatic plants is seldom reported. In our research the seeds and seedlings of the submerged macrophyte Vallisneria natans were exposed to different concentrations of microcystin-RR, which was purified with high-performance liquid chromatography (HPLC). The results indicated that microcystin-RR could accumulate differentially in the roots and leaves of V. natans seedlings. Toxin accumulation in the roots and leaves was time- and dose-dependent, with higher uptake detected in the roots. Growth and development detection revealed that V. natans was relatively insensitive to microcystin-RR at concentrations ranging from 0.0001 to 0.01 mg/L. However, when the toxin concentration was more than 0.01 mg/L, both the fresh weight and the longest leaf length of seedlings were significantly reduced after a 30-day treatment. The root and leaf numbers were significantly decreased when 10 mg/L of toxin was used. These results suggest that microcystin-RR can be taken up by V. natans, which subsequently will retard its development. PMID:15892062

  4. Influence of postharvest hot water treatment on nutritional and functional properties of kumquat (Fortunella japonica Lour. Swingle Cv. Ovale) fruit.

    Science.gov (United States)

    Schirra, Mario; Palma, Amedeo; D'Aquino, Salvatore; Angioni, Alberto; Minello, Elisabeth V; Melis, Marinella; Cabras, Paolo

    2008-01-23

    The present study investigated the influence of a hot water dip (HWD) for 2 min at 50 degrees C, a standard and effective treatment for postharvest decay control of citrus fruit, on the nutritional and health-related properties of kumquats. The results show that most of the parameters examined, including titratable acidity, soluble solids content, maturity index, glucose, fructose, sucrose, ascorbic acid, dehydroascorbic acid, alpha- and gamma-tocopherols, beta-carotene, zeaxantin, rhoifolin, and antioxidant activity, were not significantly affected by treatment. The levels of beta-cryptoxanthin, narirutin, and total flavonoids increased after HWD, whereas lutein and total phenols decreased. The concentration of the essential oil and the relative percentage of the individual components of the essential oil were not affected by HWD except for the minor compound p-menta-1,5-dien-1-ol, which increased after HWD. After storage, lower levels of glucose, total sugars, beta-carotene, beta-cryptoxanthin and lutein were recorded in HWD fruit. A decrease in antioxidant activity and increases in alpha-tocopherol and total vitamin E were found both in control and HWD fruit. The influence of HWD at 50 degrees C for 2 min on individual nutraceuticals and health-related properties was thus generally low and may depend on storage conditions.

  5. Estudo da interferência de óleos essenciais sobre a atividade de alguns antibióticos usados na clínica Study of the interference of essential oils on the activity of some antibiotic used clinically

    Directory of Open Access Journals (Sweden)

    Rinalda A. Guerra de Oliveira

    2006-03-01

    Full Text Available O objetivo deste estudo foi investigar a interferência dos óleos essenciais de Lippia sidoides Cham, Plectranthus amboinicus Lour Spr., Conyza bonariensis L. e Eucalyptus citriodora Hook sobre o efeito de antibióticos utilizados na clínica. Os ensaios foram realizados com ampicilina (10 µg/mL, cefalotina (30 µg/mL, cloranfenicol (30 µg/mL, gentamicina (10 µg/mL e tetraciclina (30 µg/mL isolados e em associação com os óleos essenciais (4% v/v através do método de difusão em meio sólido utilizando discos de papel de filtro. Os resultados mostraram interferência de alguns óleos essenciais sobre a atividade dos antibióticos ensaiados. Observou-se o desenvolvimento de halos de inibição do crescimento bacteriano com diferentes diâmetros quando da aplicação de antibióticos isolados e em combinação com os óleos essenciais. A ocorrência de comportamento sinérgico ou antagônico foi observada mais proeminentemente nas interações com cepas de S. aureus e S. epidermidis. De outra forma, observou-se uma menor interferência dos óleos essenciais no efeito dos antibióticos sobre as cepas Gram negativas, principalmente P. aeruginosa. Estes resultados mostram que o uso de produtos derivados de plantas pode em algumas situações interferir sobre a efetividade de antibióticos de uso no tratamento clínico.The aim of this study was to evaluate the interference of essential oils from Lippia sidoides Cham, Plectranthus amboinicus Lour Spr., Conyza bonariensis L. and Eucalyptus citriodora Hook on the antibacterial effect of clinically used antibiotics. The assays were carried out with ampicillin (10 µg/mL, cephalotin (30 µg/mL, chloraphenicol (30 µg/mL, gentamicin (10 µg/mL and tetracyclin (30 µg/mL isolated and associated with essential oils (4% v/v by the solid medium diffusion procedure using filter paper discs. The results showed interference of some essential oils on the antibacterial activity of the assayed antibiotics

  6. Uji Antagonis Tanaman Bangun-Bangun (Plectranthus amboinicus L.) Fungisida Nabati terhadap Penyakit Jamur Akar Putih (Rigidoporus microporus Swartz) di Laboratorium dan di Lapangan

    OpenAIRE

    Siregar, Neny Yanti

    2016-01-01

    This experimentwas aimed to obtain the optimal concentrations of the bangun-bangun extract in laboratory and to determine the inhibition potential of bangun-bangun plant against R. microporus directly on the rubber stum in polybag. The experiment was conducted in the laboratory and experimental garden of Sungei Putih Research with ± 80 m above mean sea level (amsl), from October 2015 to January 2016. Experiment in the laboratory used Completely Randomized Design (CRD) Non Factorial with thirt...

  7. Validação da metodologia de quantificação espectrofotométrica das saponinas de Pfaffia glomerata (Spreng. Pedersen - Amaranthaceae

    Directory of Open Access Journals (Sweden)

    C.L.S. Vigo

    2003-01-01

    Full Text Available Realizou-se validação analítica espectrofotométrica das saponinas totais das raízes de Pfaffia glomerata. Em testes iniciais utilizou-se saponina Merck em cinco concentrações (0,08-0,28 mg/ml para obtenção da curva de calibração e verificação da linearidade, repetibilidade e reprodutibilidade. As raízes foram avaliadas em extratos hidroalcoólicos purificados com n-butanol. O reagente cromogênico escolhido foi Cloreto de Cobalto 0,2% em pico experimental em 284nm. O tempo de leitura ideal foi de 10-20 minutos após o início da reação. O método mostrou linearidade para a faixa de concentrações utilizadas (R²= 0,9963. A precisão (coeficientes de variação situou-se entre 7-12%, indicando boa reprodutibilidade. O limite de quantificação situou-se em 0,08 mg/ml e o limite de detecção em 0,02 mg/ml. A exatidão do procedimento forneceu erro relativo de 6,3; 3,4; 3,5; 1,8 e 0,3% para as concentrações utilizadas. Os dados da curva-padrão foram aplicados em várias amostras de raízes levando ao valor média de 13,5±2,0%. Os dados obtidos permitem a quantificação espectrofotométrica das saponinas totais das raízes de P. glomerata.

  8. Genetic resources of the functional food, teramnus labialis (L.f.) spreng for improving seed number, flavonol content, oil %, and fatty acid compositions

    Science.gov (United States)

    Teramnus labialis is used as food in India and has potential to be used as a functional food vegetable in the U.S.A. Photoperiod-sensitive T. labialis accessions were grown in the greenhouse from 2010 to 2011 and evaluated for flavonol content, oil %, and fatty acid compositions. Significant variati...

  9. Larvicidal activity of Ipomoea cairica (L.) Sweet and Ageratina adenophora (Spreng.) King & H. Rob. plant extracts against arboviral and filarial vector, Culex quinquefasciatus Say (Diptera: Culicidae).

    Science.gov (United States)

    Samuel, Lallianrawna; Lalrotluanga; Muthukumaran, Rajendra Bose; Gurusubramanian, Guruswami; Senthilkumar, Nachimuthu

    2014-06-01

    Culex quinquefasciatus Say, an arboviral and filarial vector, is one of the most widespread mosquitoes in the world, and insecticide-resistant populations have been reported worldwide. Due to the emergence of resistance in C. quinquefasciatus plant based products or plant extracts may be alternative sources in integrated vector management program. The present study was carried out to establish the larvicidal activities of crude solvent extracts prepared from flowers and leaves of Ipomoea cairica and Ageratina adenophora against third instar larvae of C. quinquefasciatus as target species. The plant extracts were prepared with petroleum ether, chloroform and methanol solvents using sequential extraction method to determine the best extractant for subsequent isolation and characterization of active ingredient. The total yield of plant extract in the Soxhlet extraction ranged between 0.79% and 19.35%. The qualitative phytochemical study of the plant extracts from different solvents showed the presence of alkaloids, flavonoids, phenols, terpenes, saponins and tannins in different combinations. I. cairica and A. adenophora plant extracts were found to be effective against third instar larvae of C. quinquefasciatus causing 77-100% mortality at 48h. Highest mortality was observed at 500ppm and the order of larvicidal action was observed to be of methanol extract of I. cairica flower>petroleum ether extract of A. adenophora leaf>chloroform extract of I. cairica leaf. High mortality (100%) with low LC50 and LT50 were observed in methanolic flower extract (LC50 - 8.43ppm; LT50 - 2.51h at 48h) of I. cairica, and petroleum ether (LC50 - 133.56ppm; LT50 - 9.45h at 48h) leaf extract of A. adenophora. Lethal concentration (LC50 and LC90) values gradually decreased with the exposure periods, lethal time (LT50 and LT90) decreased with the concentration in bioassay experiment with the crude plant extracts. There was a significant correlation (three-way factorial ANOVA) was noticed among concentration of the plant extracts, exposure time and solvent extraction in relation to larval mortality (Pactive components present in the plant solvent extracts and implement the effective arboviral and filarial mosquito vector management program.

  10. Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae: metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.

    Directory of Open Access Journals (Sweden)

    Poliana Cardoso-Gustavson

    Full Text Available Here, we proposed that volatile organic compounds (VOC, specifically methyl salicylate (MeSA, mediate the formation of calcium oxalate crystals (COC in the defence against ozone (O3 oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O3 that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O3.

  11. Ozone-Induced Responses in Croton floribundus Spreng. (Euphorbiaceae): Metabolic Cross-Talk between Volatile Organic Compounds and Calcium Oxalate Crystal Formation

    OpenAIRE

    Poliana Cardoso-Gustavson; Vanessa Palermo Bolsoni; Debora Pinheiro de Oliveira; Maria Tereza Gromboni Guaratini; Marcos Pereira Marinho Aidar; Mauro Alexandre Marabesi; Edenise Segala Alves; Silvia Ribeiro de Souza

    2014-01-01

    Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morph...

  12. Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.

    Science.gov (United States)

    Cardoso-Gustavson, Poliana; Bolsoni, Vanessa Palermo; de Oliveira, Debora Pinheiro; Guaratini, Maria Tereza Gromboni; Aidar, Marcos Pereira Marinho; Marabesi, Mauro Alexandre; Alves, Edenise Segala; de Souza, Silvia Ribeiro

    2014-01-01

    Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA) regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O3) that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O3. PMID:25165889

  13. Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.

    Science.gov (United States)

    Cardoso-Gustavson, Poliana; Bolsoni, Vanessa Palermo; de Oliveira, Debora Pinheiro; Guaratini, Maria Tereza Gromboni; Aidar, Marcos Pereira Marinho; Marabesi, Mauro Alexandre; Alves, Edenise Segala; de Souza, Silvia Ribeiro

    2014-01-01

    Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA) regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O3) that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O3.

  14. Influence of salt stress on essential oil yield and composition of lemon grass (Cymbopogon schoenanthus L. Spreng. ssp. Laniger (Hook) Maire et Weil).

    Science.gov (United States)

    Khadhri, Ayda; Neffati, Mohamed; Smiti, Samira; Nogueira, José Manuel F; Araujo, Maria Eduarda M

    2011-01-01

    Cymbopogon is an aromatic plant valued for its citrus scent aroma. In this article, the effect of saline irrigation water on yield and quality of Cymbopogon schoenanthus L. was evaluated. Compounds of essential oils were identified by gas chromatography-mass spectrometry and/or (13)C-NMR spectroscopy. Results showed that the growth of the aerial part was not affected at a concentration of 50 mmol NaCl. Under salt stress, the content of major chemical compounds was affected differently by the treatment level. PMID:21246437

  15. Avaliação dos efeitos das raízes de Pfaffia glomerata (Spreng. Pedersen sobre o tempo de sono e crescimento bacteriano

    Directory of Open Access Journals (Sweden)

    C.L.S. Vigo

    2003-01-01

    Full Text Available Foram realizados testes biológicos com extrato hidroalcoólico liofilizado das raízes de Pfaffia glomerata. O teste de tempo de sono em camundongos envolveu administração de 50 mg/kg de pentobarbital sódico (via i.p.. O extrato foi administrado em várias doses agudamente e após 10 e 30 dias de tratamento oral. No teste antibacteriano, pequena quantidade do extrato de P. glomerata foi misturado com o inóculo de Staphylococcus aureus, Escherichia coli, Bacillius subtilis e Pseudomonas aeruginosa diluídos 1:10 para a microdiluição em caldo. No teste de tempo de sono, no modelo agudo obteve-se efeitos depressores para as doses de 500 e 1000 mg/kg; nos animais tratados cronicamente, aos 10 dias de tratamento obteve-se efeito depressor com 50 mg/kg e efeito estimulante com 500mg/kg; aos 30 dias de tratamento outro efeito estimulante ocorreu com a dose de 1 mg/kg. No teste antibacteriano não se observou inibição de crescimento de nenhuma das amostras das bactérias na maior concentração de 1000μg/ml do extrato. Conclui-se que o extrato das raízes de P. glomerata não tem efeito antimicrobiano e parece promover alguma interferência sobre o sono de animais de modo bifásico de acordo com as doses e tempos de tratamento.

  16. Crescimento inicial de mudas de Clitoria fairchildiana Howard e Peltophorum dubium (Sprenge Taub em diferentes condições de sombreamento

    Directory of Open Access Journals (Sweden)

    Rita C.Q. Portela

    2001-01-01

    Full Text Available Este experimento teve como objetivo avaliar a influência do sombreamento no crescimento de mudas de Peltophorum dubium (Canafístula e Clitoria fairchildiana (Sombreiro, espécies utilizadas no reflorestamento de encostas do município do Rio de Janeiro. As sementes foram colhidas em diversas matrizes e semeadas em sacos plásticos. As porcentagens de sombreamento testadas foram: 0% (sol pleno, 30%, 50% e 75%. A instalação do experimento iniciou-se um mês após a semeadura. O parâmetro altura foi analisado aos 30, 60, 90, 120 e 150 dias após a semeadura. O peso da matéria seca da raiz foi analisado aos 150 dias. Os resultados demostraram que as espécies estudadas apresentam diferentes respostas para os parâmetros analisados. Para o plantio imediato recomenda-se a produção de mudas de Sombreiro sob o sombreamento de 30% e de Canafístula, sob sol pleno, 30% ou 75% de sombra. Na produção de mudas para estocagem, seria indicado para o Sombreiro, a produção a sol pleno e para Canafístula, as mudas poderiam ser mantidas sob 50% de sombreamento. Ambas as espécies podem ser plantadas sob pleno sol, 30%, 50% e 75% de sombreamento, sendo indicadas para a implantação de reflorestamentos ou para enriquecimento de área degradadas.

  17. Crescimento inicial de mudas de Clitoria fairchidiana Howard e Peltophorum dubium (Sprenge Taub em diferentes condições de sombreamento.

    Directory of Open Access Journals (Sweden)

    Rita C.Q. Portela

    2010-08-01

    Full Text Available Normal 0 21 false false false PT-BR X-NONE X-NONE MicrosoftInternetExplorer4 Este experimento teve como objetivo avaliar a influência do sombreamento no crescimento de mudas de Peltophorum dubium (Canafístula e Clitoria fairchildiana (Sombreiro, espécies utilizadas no reflorestamento de encostas do município do Rio de Janeiro. As sementes foram colhidas em diversas matrizes e semeadas em sacos plásticos. As porcentagens de sombreamento testadas foram: 0% (sol pleno, 30%, 50% e 75%. A instalação do experimento iniciou-se um mês após a semeadura. O parâmetro altura foi analisado aos 30, 60, 90, 120 e 150 dias após a semeadura. O peso da matéria seca da raiz foi analisado aos 150 dias. Os resultados demostraram que as espécies estudadas apresentam diferentes respostas  para os parâmetros analisados. Para o plantio imediato recomenda-se a produção de mudas de Sombreiro sob o sombreamento de 30% e de Canafístula, sob sol pleno, 30% ou 75% de sombra. Na produção de mudas para estocagem, seria indicado para o Sombreiro, a produção a sol  pleno e para Canafístula, as mudas poderiam ser mantidas sob 50% de sombreamento. Ambas as espécies podem ser plantadas sob pleno sol, 30%, 50% e 75% de sombreamento, sendo indicadas para a implantação de reflorestamentos ou  para enriquecimento de área degradadas.

  18. Effects of Mikania glomerata Spreng. and Mikania laevigata Schultz Bip. ex Baker (Asteraceae) extracts on pulmonary inflammation and oxidative stress caused by acute coal dust exposure

    Energy Technology Data Exchange (ETDEWEB)

    Freitas, T.P.; Silveira, P.C.; Rocha, L.G.; Rezin, G.T.; Rocha, J.; Citadini-Zanette, V.; Romao, P.T.; Dal-Pizzol, F.; Pinho, R.A.; Andrade, V.M.; Streck, E.L. [University Extremo Catarinense, Criciuma (Brazil)

    2008-12-15

    Several studies have reported biological effects of Mikania glomerata and Mikania laevigata, used in Brazilian folk medicine for respiratory diseases. Pneumoconiosis is characterized by pulmonary inflammation caused by coal dust exposure. In this work, we evaluated the effect of pretreatment with M. glomerata and M. laevigata extracts (MGE and MLE, respectively) (100 mg/kg, s.c.) on inflammatory and oxidative stress parameters in lung of rats subjected to a single coal dust intratracheal instillation. Rats were pretreated for 2 weeks with saline solution, MGE, or MLE. On day 15, the animals were anesthetized, and gross mineral coal dust or saline solutions were administered directly in the lung by intratracheal instillation. Fifteen days after coal dust instillation, the animals were killed. Bronchoalveolar lavage (BAL) was obtained; total cell count and lactate dehydrogenase (LDH) activity were determined. In the lung, myeloperoxidase activity, thiobarbituric acid-reactive substances (TBARS) level, and protein carbonyl and sulfhydryl contents were evaluated. In BAL of treated animals, we verified an increased total cell count and LDH activity. MGE and MLE prevented the increase in cell count, but only MLE prevented the increase in LDH. Myeloperoxidase and TBARS levels were not affected, protein carbonylation was increased, and the protein thiol levels were decreased by acute coal dust intratracheal administration. The findings also suggest that both extracts present an important protective effect on the oxidation of thiol groups. Moreover, pretreatment with MGE and MLE also diminished lung inflammatory infiltration induced by coal dust, as assessed by histopathologic analyses.

  19. Plasticidade fenotípica de Baccharis genistelloides subsp. crispa (Spreng. Joch. Müll. (2006 - Asteraceae - sob manejo orgânico

    Directory of Open Access Journals (Sweden)

    L.A. Sousa

    2014-06-01

    Full Text Available O objetivo deste trabalho, relacionado a produção orgânica, utilizou preparados homeopáticos visando incremento na biomassa e no teor de flavonóides em plantas dióicas de carqueja. Os tratamentos foram: controle com água, controle com álcool 70%, Phosphorus 12CH, Sulphur 6CH, e a combinação destas homeopatias. Variações fenotípicas foram registradas a partir da análise de crescimento, realizada quinzenalmente durante 60 dias e dos caracteres morfológicos, tais como: como altura, número de ramificações, comprimento do ramo principal, diâmetro do caule e ala caulinar mediana. Na colheita foram medidos: a fitomassa fresca/seca e o volume de raiz. Posteriormente, foi quantificado o teor de flavonóides totais conforme as análises prescritas pela Farmacopéia Brasileira. O experimento foi avaliado em delineamento de blocos casualizados (2x5x2, sendo suas médias avaliadas pelo teste Tukey e o coeficiente de variação ambiental para estimar a plasticidade. Quanto ao estudo do crescimento da planta ao longo do tempo, verificou-se aumento linear simples para a maioria das variáveis nos dois sexos. Foram detectadas respostas plásticas na maioria dos caracteres avaliados. De acordo com os resultados, em ambos os sexos, 60 dias de cultivo são suficientes para obtenção de matéria prima com teor aceitável de flavonóides, bem como para verificação dos efeitos de patogenesia e similitude na aplicação de preparados homeopáticos Phosphorus 12CH, Sulphur 6CH e sua combinação Phosphorus 12CH + Sulphur 6CH. A fitomassa e o teor de flavonóides totais foram semelhantes entre plantas dióicas durante o período de estudo.

  20. Ozone-Induced Responses in Croton floribundus Spreng. (Euphorbiaceae): Metabolic Cross-Talk between Volatile Organic Compounds and Calcium Oxalate Crystal Formation

    Science.gov (United States)

    Cardoso-Gustavson, Poliana; Bolsoni, Vanessa Palermo; de Oliveira, Debora Pinheiro; Guaratini, Maria Tereza Gromboni; Aidar, Marcos Pereira Marinho; Marabesi, Mauro Alexandre; Alves, Edenise Segala; de Souza, Silvia Ribeiro

    2014-01-01

    Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA) regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O3) that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O3. PMID:25165889

  1. Responsabilidade social… administrativa um plano de resposta a preocupações económicas, sociais e ambientais para os SMAS de Loures

    OpenAIRE

    Santos, Sílvia Cristina Ribeiro Mendes dos

    2011-01-01

    Trabalho de projecto, Mestrado em Administração Pública A Responsabilidade Social é, cada vez mais, um mecanismo utilizado pelas empresas, que encontram neste meio uma forma de dar resposta a preocupações económicas, sociais e ambientais. Por seu lado, as organizações públicas encontram-se perante um novo paradigma baseado em valores que se traduzem numa gestão cada vez mais qualitativa e “humana” dos recursos públicos, num contexto que nos parece totalmente adequado à adop...

  2. Polyploid Induction of Buddleja asiatica Lour. by Colchicine%秋水仙素诱导七里香多倍体

    Institute of Scientific and Technical Information of China (English)

    陈高; 孙卫邦

    2006-01-01

    以不同浓度(0.02%、0.04%、0.08%、0.16%)秋水仙素溶液与二甲基亚砜和丙草胺的体积混合比为2000:10:l的溶液诱导七里香种子萌发苗1~3d的结果表明,所有处理均得到七里香多倍体,其中以秋水仙素浓度为0.08%的混合液处理3 d的诱导效果最好,多倍体诱导率达36.7%.

  3. Efecto de la profundidad del suelo en rottboellia cochinchinensis (lour clayton en caña de azúcar (saccharum officinarum l.

    Directory of Open Access Journals (Sweden)

    Ram\\u00F3n Le\\u00F3n

    2001-01-01

    Full Text Available Se muestrearon cuatro lotes con diferente textura de suelo, sembrados con caña de azúcar durante cuatro años consecutivos. El lote A con suelo franco arcilloso, el lote B con suelo franco arenoso, el lote C con suelo arcilloso y el lote D con suelo franco. Se seleccionaron lotes con una población de R. cochinchinensis de 75 a 150 plantas/ m2. Se contó el número de plantas de la maleza en un área de 0,25 m2, en dos micro-hábitats: el surco y entre-surco. A cada planta se le escarbó el suelo que la rodeaba hasta encontrar la semilla de la que provenía y se midió la profundidad a la que ésta se encontró, para luego determinar qué porcentaje del total de plantas contabilizadas provenía de semillas localizadas en cada uno de los siguientes estratos: 0,0; >0,0 a 2,5; >2,5 a 5,0; >5,0 a 10 y >10,0 a 15,0 cm de profundidad. Además, se evaluó en invernadero el efecto de cada profundidad de los estratos citados. A nivel de laboratorio y en platos Petri, se evaluó el efecto de la luz y la oscuridad sobre la germinación de la semilla de R. cochinchinensis. Se encontró que la mayoría de las plantas en campo provenían de semillas que se encontraban de >0 a 2,5 cm, seguido por aquellas que estaban de >2,5 a 5,0 cm donde se dieron las mejores condiciones para la germinación. No se observaron diferencias entre los tipos de suelo o los micro-hábitats evaluados. En invernadero el tratamiento a 0,0 cm fue el que mostró la mayor germinación y esto fue producto de la exposición a la luz, lo cual se corroboró en el estudio en laboratorio donde el tratamiento con luz presentó también, la mayor germinación

  4. Production and Metabolism of Indole Acetic Acid in Root Nodules and Symbiont (Rhizobium undicola Isolated from Root Nodule of Aquatic Medicinal Legume Neptunia oleracea Lour.

    Directory of Open Access Journals (Sweden)

    Pallab Kumar Ghosh

    2015-01-01

    Full Text Available Indole acetic acid is a phytohormone which plays a vital role in plant growth and development. The purpose of this study was to shed some light on the production of IAA in roots, nodules, and symbionts of an aquatic legume Neptunia oleracea and its possible role in nodular symbiosis. The symbiont (N37 was isolated from nodules of this plant and identified as Rhizobium undicola based on biochemical characteristics, 16S rDNA sequence homology, and DNA-DNA hybridization results. The root nodules were found to contain more IAA and tryptophan than root; however, no detectable amount of IAA was found in root. The IAA metabolizing enzymes IAA oxidase, IAA peroxidase (E.C.1.11.1.7, and polyphenol oxidase (E.C.1.14.18.1 were higher in root than nodule but total phenol and IAA content were reversed. The strain N37 was found to produce copious amount of IAA in YEM broth medium with tryptophan and reached its stationary phase at 20 h. An enrichment of the medium with mannitol, ammonium sulphate, B12, and 4-hydroxybenzaldehyde was found to promote the IAA production. The presence of IAA metabolizing enzymes and IAA production with PGPR traits including ACC deaminase activity of the symbionts was essential for plant microbe interaction and nodule function.

  5. MicroRNA390-directed TAS3 cleavage leads to the production of tasiRNA-ARF3/4 during somatic embryogenesis in Dimocarpus longan Lour.

    Directory of Open Access Journals (Sweden)

    Yuling eLin

    2015-12-01

    Full Text Available Trans-acting short-interfering RNAs (tasiRNAs originate from TAS3 families through microRNA (miRNA 390-guided cleavage of primary transcripts and target auxin response factors (ARF3/-4, which are involved in the normal development of lateral roots and flowers in plants. However, their roles in embryo development are still unclear. Here, the pathway miR390-TAS3-ARF3/-4 was identified systematically for the first time during somatic embryo development in Dimocarpus longan. We identified the miR390 primary transcript and promoter. The promoter contained cis-acting elements responsive to stimuli such as light, salicylic acid, anaerobic induction, fungal elicitor, circadian control and heat stress. The longan TAS3 transcript, containing two miR390-binding sites, was isolated; the miR390- guided cleavage site located near the 3' end of the TAS3 transcript was verified. Eight TAS3-tasiRNAs with the 21-nucleotide phase were found among longan small RNA data, further confirming that miR390-directed TAS3 cleavage leads to the production of tasiRNA in longan. Among them, TAS3_5'D5+ and 5'D6+ tasiRNAs were highly abundant, and verified to target ARF3 and -4, implying that miR390-guided TAS3 cleavage with 21-nucleotide phase leading to the production of tasiRNA-ARF is conserved in plants. Pri-miR390 was highly expressed in friable-embryogenic callus (EC, and less expressed in incomplete compact pro-embryogenic cultures,while miR390 showed its lowest expression in EC and highest expression in torpedo-shaped embryo. DlTAS3 and DlARF4 both exhibited their lowest expressions in EC, and reached their peaks in the globular embryos stage, which were mainly inversely proportional to the expression of miR390, especially at the GE to CE stages. While DlARF3 showed little variation from the EC to torpedo-shaped embryos stages, and exhibited its lowest expression in the cotyledonary embryos stage. There was a general lack of correlation between the expressions of DlARF3 and miR390. In addition, miR390, DlTAS3, DlARF3, and -4 were up-regulated by 2,4-D in a concentration-dependent manner. They were also preferentially expressed in roots, pulp, and seeds of ‘Sijimi’ longan, implying their extended roles in the development of longan roots and fruit. This study provided insights into a possible role of miR390-tasiRNAs-ARF in plant somatic embryo development.

  6. Comparative immunomodulation potential of Tinospora cordifolia (Willd.) Miers ex Hook. F., Tinospora sinensis (Lour.) Merrill and Tinospora cordifolia growing on Azadirachta indica A. Juss.

    Science.gov (United States)

    Narkhede, A N; Jagtap, S D; Kasote, D M; Kulkarni, O P; Harsulkar, A M

    2014-08-01

    Guduchi has been widely used in the traditional medicine as an immunomodulator. Description of guduchi in Ayurvedic literature resemble with T. sinensis rather than with commonly available T. cordifolia and hence this may be used as substitutes for T. sinensis. T. cordifolia growing on Azadirachta indica commonly called Neem-guduchi has more immunomodulatory potential. Thus, immunomodulatory activity of three Tinospora spp. was assessed by checking humoral and cell mediated immune responses to the antigenic challenges with sheep RBCs and by neutrophil adhesion tests on albino Wistar rats using Guduchi-Satwa, a well known dosage form. Results revealed that Neem-guduchi possesses higher immunomodulatory potential at the dose of 300 mg/kg, po and validated the traditional claim. Hence, Neem-Guduchi can be employed in immunomodulatory formulation prepared using guduchi. PMID:25141544

  7. 中华青牛胆扦插繁育试验研究%Experimental Study on Cutting Propagation of Tinospora sinensis (Lour.) Merr.

    Institute of Scientific and Technical Information of China (English)

    兰玲; 李融兴

    2008-01-01

    [目的]为中华青牛胆的扦插育苗提供理论依据.[方法]以野生中华青牛胆1 年生健壮枝为插穗,设置3个因素(扦插基质、激素、插穗粗度)3个水平的正交试验.研究不同处理对中华青牛胆生根率、生根量以及根长度的影响.[结果]不同因素对中华青牛胆扦插生根率的影响依次为ABT 1号生根粉>基质>插穗直径.影响中华青牛胆插穗单株平均根长的主要因素是基质,以黄心土+河沙混合基质为最好;插穗直径的影响最小.影响插穗生根量的主要因素是插穗直径,插穗粗度以15.0 mm最佳,其次是基质和ABT号生根粉. [结论]中华青牛胆的扦插育苗最佳处理组合为(黄心土+河沙)+ABT 1号生根粉200 mg/L+插穗直径15.0 mm;(黄心土+河沙)+ABT 1号生根粉100 mg/L+插穗直径15.0 mm.

  8. Physicochemical characteristcs and enzymatic activity of peroxidase and polyphenoloxidase in four genotypes of cupuaçu (Theobroma grandiflorum Willd ex-Spreng Schum submitted to freezingCaracterísticas físico-químicas e atividade da peroxidase e polifenoloxidase em genótipos de cupuaçu (Theobroma grandiflorum Willd ex-Spreng Schum submetidos ao congelamento

    Directory of Open Access Journals (Sweden)

    Salomão Rocha Martim

    2013-10-01

    Full Text Available During the freezing of fruits pulps, the enzyme activity is not finished completely. Sensory, nutritional and coloring changes may occur on fruits due to the action of oxidative enzymes such as peroxidase and polyphenoloxidase. The frozen cupuaçu pulps, sold in Brazil, have a shelf life of one year and become browned during this period. The aim of this study was to evaluate the effect of frozen storage on the physicochemical characteristics, polyphenoloxidase activity and soluble and ionically bound peroxidases presented in the pulps of four new cupuaçu genotypes over twelve months. The cupuaçu genotypes developed by the West Amazonian Agroforestry Research Center (EMBRAPA were pulped, frozen and stored at – 30 °C. The polyphenoloxidase of the four cupuaçu genotypes showed an increase in activity according to the storage time with peaks in the sixth, ninth and tenth months, but the peroxidases exhibited oscillations in the enzyme activity. The physicochemical properties of the pulps showed variations during the twelve months of storage under freezing. The vitamin C content of D 28-10 and P 3-10 genotypes decreased from the fourth and tenth months, respectively. Moreover P 9-8 e B 28-7 genotypes remained stable. In relation the acidity of citric acid, the B-28-7, D 28-10 and P 9-8 samples were not different, but P 3-10 genotype presented a reduction. The pH and total soluble solids of all genotypes decreased over the study period. There was an increase in sugar concentration of B 28-7, P 3-10 and P 9-8 genotypes, except for D 28-10 sample which remained unchanged. All genotypes were in accordance with physical-chemicals standards required by legislation, except for P 3-10 genotype that showed a lower acidity. In respect of the enzymatic parameters, there were variations in the activity of peroxidase and polyphenoloxidases of all genotypes. No congelamento de polpas de frutas a atividade enzimática não é completamente cessada. Podem ocorrer mudanças sensoriais, nutricionais e de coloração devido à ação de enzimas oxidativas, como a peroxidase e a polifenoloxidase. Considerando que as polpas de cupuaçu congeladas, comercializadas no Brasil, têm um prazo de validade de um ano e tornam-se escurecidas ao longo deste período, objetivou-se neste estudoavaliar o efeito do tempo de congelamento nas características físico-químicas e nas atividades da polifenoloxidase e peroxidases solúvel e insolúvel presentes nas polpas de quatro novos genótipos de cupuaçu, durante doze meses. Os frutos dos genótipos de cupuaçu, desenvolvidos pela Embrapa Amazônia Ocidental, foram despolpados, congelados e armazenados à temperatura de –30 ºC. A polifenoloxidase das polpas dos quatro genótipos apresentou aumento na sua atividade com picos no sexto, nono e décimo mês e as peroxidases apresentaram oscilações na atividade enzimática. As propriedades físico-químicas das polpas apresentaram variações durante os doze meses de armazenamento sob congelamento. O teor de vitamina C dos genótipos D 28-10 e P 3-10 diminuiu a partir do 4º e 10º mês, respectivamente. Por outro lado os genótipos B 28-7 e P 9-8 permaneceram estáveis. Em relação à acidez em ácido cítrico, as amostras B-28-7, D 28-10 e P 9-8 não diferiram, havendo redução no genótipo P 3-10. O valores de pH e sólidos solúveis totais de todos os genótipos diminuíram ao longo do período avaliado. Houve aumento na concentração de açúcares das polpas dos genótipos B 28-7, P 3-10 e P 9-8, com exceção da amostra D 28-10 que permaneceu inalterada. Todos os genótipos apresentaram-se dentro dos padrões físico-químicos exigidos pela legislação, com exceção do genótipo P 3-10 que apresentou acidez inferior. Em relação aos parâmetros enzimáticos, houve variações na atividade das peroxidases e polifenoloxidases de todos os genótipos avaliados.

  9. Produção de β-ecdisona em Pfaffia glomerata (Spreng. Pedersen em função da adubação orgânica em 6 épocas de crescimento β-ecdysone production by Pfaffia glomerata (Spreng. Pedersen under organic fertilization in 6 growth periods

    Directory of Open Access Journals (Sweden)

    C.P.V. Guerreiro

    2009-01-01

    Full Text Available O trabalho teve como objetivo verificar a influência da adubação orgânica em 6 épocas de crescimento na produção de β-ecdisona por plantas de Pfaffia glomerata. O experimento foi conduzido na Fazenda Santo Antonio do Araquá, distrito de Catâneo Ângelo, município de São Manuel, São Paulo, Brasil. Utilizou-se o delineamento de blocos ao acaso, num esquema fatorial 5x6, com quatro repetições, considerando-se 8 plantas úteis por parcela. Os blocos foram constituídos de 6 épocas de crescimento (60, 120, 180, 240, 300 e 360 dias após a germinação e de 5 doses de esterco de galinha curtido [testemunha (sem adubação, 15, 30, 45 e 60 t ha-1]. Após cada colheita, as raízes das plantas foram secas em estufa com circulação de ar forçada a 40ºC e pesadas para posterior extração do β-ecdisona, seguindo metodologia desenvolvida por Magalhães (2000. Os resultados foram submetidos à análise de variância e ao teste de separação de médias de Scott Knott, todos a 5% de probabilidade. Quando ocorreu interação os resultados foram avaliados usando-se análise de regressão polinominal. O teor de β-ecdisona não foi influenciado pelas doses de adubo e nem pela época do crescimento das plantas. Porém a quantidade total de β-ecdisona por raiz foi influenciada pela época de crescimento, sendo que aos 360 dias após a emergência ocorreu uma maior quantidade do princípio ativo em todos os tratamentos. Apesar de não diferir estatisticamente dos demais tratamentos, aos 360 dias após a emergência das plantas, o tratamento 30 t ha-1 foi o que proporcionou maior quantidade de β-ecdisona.The aim of this study was to assess the influence of organic fertilization in 6 growth periods on β-ecdysone production by Pfaffia glomerata plants. The field work was conducted in "Santo Antonio do Araquá" Farm, Catâneo Ângelo District, São Manuel, São Paulo State, Brazil. The adopted design was in randomized blocks, in a 5x6 factorial arrangement, with four replicates and 8 useful plants per plot. Blocks were constituted of 6 growth periods (60, 120, 180, 240, 300 and 360 days after germination, and 5 chicken manure levels [control (without fertilization, 15, 30, 45 and 60 t ha-1]. After each harvest, roots were dried in forced aeration oven at 40ºC and weighed for later β-ecdysone extraction following the methodology developed by Magalhães (2000. Results were subjected to analysis of variance and mean separation by Scott-Knott test, both at 5% probability. When interaction was detected, results were evaluated using polynomial regression analysis. β-ecdysone content was not influenced by manure levels or plant growth periods. However, β-ecdysone total content per root was influenced by the growth period, and at 360 days after emergence there was a higher quantity of the active principle in all treatments. Although not statistically different from the remaining treatments, 30 t ha-1 led to the highest β-ecdysone production at 360 days after plant emergence.

  10. Utilização de parâmetros morfoanatômicos na análise da fitotoxidez do flúor em folhas de Magnolia ovata (A. St.-Hil. Spreng. (Magnoliaceae Use of morphoanatomic parameters in the analysis of fluoride toxicity in leaves of Magnolia ovata (A. St.-Hil. Spreng. (Magnoliaceae

    Directory of Open Access Journals (Sweden)

    Bruno Francisco Sant'Anna-Santos

    2007-08-01

    Full Text Available Com os objetivos de avaliar o grau de suscetibilidade e caracterizar as injúrias na morfoanatomia de folhas de Magnolia ovata, mudas foram submetidas à chuva simulada com flúor (10 µg.ml-1 de F- por 10 dias consecutivos. No tratamento controle, utilizou-se apenas água deionizada. Folhas foram coletadas para quantificação de flúor na matéria seca e fixadas para análises em microscopia de luz e eletrônica de varredura. As folhas apicais apresentaram pequena porcentagem de necroses intervenais e marginais em uma ou ambas as faces e maior acúmulo de flúor, em relação ao controle, quando comparadas com as folhas da porção basal. A análise micromorfológica das folhas aparentemente sadias indicou alterações nas paredes periclinais externas da epiderme e formação de concavidades, além de cristas estomáticas danificadas, erosão de ceras epicuticulares e presença de esporos e hifas de fungos. A caracterização estrutural das injúrias evidenciou retração de protoplasto das células epidérmicas, colapso das células do mesofilo e da epiderme e acúmulo de compostos fenólicos em células das regiões necrosadas. As alterações micromorfológicas das folhas ocorreram antes que sintomas fossem observados, o que comprova a importância da micromorfologia na diagnose precoce da injúria. As plantas de M. ovata apresentaram poucos sintomas visuais em resposta ao flúor, entretanto as alterações morfoanatômicas indicam que essa espécie possui potencial para ser utilizada como bioindicadora.This work aimed to evaluate the degree of susceptibility and characterize the injuries caused by fluoride in the morphoanatomy of Magnolia ovata. Seedlings were subjected to fluoride simulated rain (10 µg.ml-1 of F- during 10 consecutive days. In the control treatment, only deionized water was used. Leaves were collected for quantification of fluoride in the dry weight and fixed for light and scanning electron microscopy analyses. The apical leaves presented a small percentage of interveinal and margin necrosis in one or both leaf surfaces and greater fluoride accumulation, in relation to the control, than the basal portion leaves. Micromorphological analyses indicated that the areas apparently healthy of epidermis presented alterations in the external periclinal walls and formation of concave shapes, beyond damaged stomatic ledge, erosion of epicuticular waxes and presence of spores and fungal hyphae. The structural characterization of the injuries showed retraction of the protoplast of the epidermal cells, collapse of the mesophyll and epidermal cells and accumulation of phenolic compounds in cells of the necrotic regions. The micromorphological variations occurred before leaf symptoms, confirming the importance of micromorphology in early diagnosis of the injury. Plants of M. ovata presented few visible symptoms in response to fluoride; however, morphoanatomic responses suggest that this species possesses potential features to be used as a bioindicator.

  11. Study of the Interference between Plectranthus Species Essential Oils from Brazil and Aminoglycosides.

    Science.gov (United States)

    Galvão Rodrigues, Fabíola Fernandes; Costa, José Galberto Martins; Rodrigues, Fábio Fernandes Galvao; Campos, Adriana Rolim

    2013-01-01

    Plectranthus is one of the most representative genera of Lamiaceae family. In this study, the essential oils from Plectranthus amboinicus, Plectranthus ornatus, and Plectranthus barbatus were investigated for their chemical composition and antimicrobial and modulatory activities. The major components found were carvacrol (54.4%-P. amboinicus) and eugenol (22.9%-P. ornatus e 25.1%-P. barbatus). In vitro antimicrobial activity was conducted against Escherichia coli, Proteus vulgaris, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus aureus (multiresistant) using microdilution method. The results of bioassay showed that all strains were sensitive to the oils, except P. aeruginosa that was resistant to P. amboinicus and P. ornatus. A synergistic effect of all essential oils combined with the aminoglycosides was demonstrated. These results show that P. amboinicus, P. ornatus, and P. barbatus inhibit the growth of pathogenic microorganism, and besides this they present antibiotic modifying activity, providing a new perspective against the problem of bacterial resistance to antibiotics. PMID:23662150

  12. Study of the Interference between Plectranthus Species Essential Oils from Brazil and Aminoglycosides

    OpenAIRE

    Fabíola Fernandes Galvão Rodrigues; José Galberto Martins Costa; Fábio Fernandes Galvao Rodrigues; Adriana Rolim Campos

    2013-01-01

    Plectranthus is one of the most representative genera of Lamiaceae family. In this study, the essential oils from Plectranthus amboinicus, Plectranthus ornatus, and Plectranthus barbatus were investigated for their chemical composition and antimicrobial and modulatory activities. The major components found were carvacrol (54.4%—P. amboinicus) and eugenol (22.9%—P. ornatus e 25.1%—P. barbatus). In vitro antimicrobial activity was conducted against Escherichia coli, Proteus vulgaris, Bacillus c...

  13. Research Progress of Resourceful Treatment of An Invasion Plant:Eupatorium adenophorum Spreng%入侵植物紫茎泽兰资源化利用研究进展

    Institute of Scientific and Technical Information of China (English)

    朱先洲

    2012-01-01

    紫茎泽兰(Eupatorium adenophorum)是我国外来入侵物种中危害最为严重的植物之一,广泛分布于我国西南地区,并有向东南部和中南部蔓延的趋势,严重影响农林业生产、威胁生物多样性安全.因此,加强对紫茎泽兰防治方法的研究显得尤为迫切.近年来,越来越多的研究发现紫茎泽兰可以进行多种不同的资源化处理,在参考了国内外较大量紫茎泽兰的资源化利用的文献资料后,综述了紫茎泽兰资源化利用的研究进展,并就其利用前景进行了探讨.%Eupatorium adenophorum is one of the most dangerous invasive plants in China. It has spread rapidly throughout south-western China and has a great potential for invading south-eastern and south-central China. It has influenced the farming and forestry production seriously,and threatened the biodiversity safety of China. Therefore, it's urgent to study the successful control method of this weed. Recently, more and more studies indicated that E. Adenophora can be adopted for many different kinds of resourceful treatments. According to the documents related with research progress of resourceful treatment of E. Adenophora. Research progress of resourceful treatment of E. Adenophora were reviewed, and their prospects were also discussed.

  14. Antioxidative ability and membrane integrity in salt-induced responses of Casuarina glauca Sieber ex Spreng. in symbiosis with N2-fixing Frankia Thr or supplemented with mineral nitrogen.

    Science.gov (United States)

    Scotti-Campos, Paula; Duro, Nuno; Costa, Mário da; Pais, Isabel P; Rodrigues, Ana P; Batista-Santos, Paula; Semedo, José N; Leitão, A Eduardo; Lidon, Fernando C; Pawlowski, Katharina; Ramalho, José C; Ribeiro-Barros, Ana I

    2016-06-01

    The actinorhizal tree Casuarina glauca tolerates extreme environmental conditions, such as high salinity. This species is also able to establish a root-nodule symbiosis with N2-fixing bacteria of the genus Frankia. Recent studies have shown that C. glauca tolerance to high salt concentrations is innate and linked to photosynthetic adjustments. In this study we have examined the impact of increasing NaCl concentrations (200, 400 and 600mM) on membrane integrity as well as on the control of oxidative stress in branchlets of symbiotic (NOD+) and non-symbiotic (KNO3+) C. glauca. Membrane selectivity was maintained in both plant groups at 200mM NaCl, accompanied by an increase in the activity of antioxidative enzymes (superoxide dismutase, ascorbate peroxidase, glutathione reductase and catalase). Regarding cellular membrane lipid composition, linolenic acid (C18:3) showed a significant decline at 200mM NaCl in both NOD+ and KNO3+ plants. In addition, total fatty acids (TFA) and C18:2 also decreased in NOD+ plants at this salt concentration, resulting in malondialdehyde (MDA) production. Such initial impact at 200mM NaCl is probably due to the fact that NOD+ plants are subjected to a double stress, i.e., salinity and low nitrogen availability. At 400mM NaCl a strong reduction of TFA and C18:3 levels was observed in both plant groups. This was accompanied by a decrease in the unsaturation degree of membrane lipids in NOD+. However, in both NOD+ and KNO3+ lipid modifications were not reflected by membrane leakage at 200 or 400mM, suggesting acclimation mechanisms at the membrane level. The fact that membrane selectivity was impaired only at 600mM NaCl in both groups of plants points to a high tolerance of C. glauca to salt stress independently of the symbiotic relation with Frankia. PMID:27070734

  15. Study on antimicrobial activity and chemical constituents of essential oil from the flowers ofMicheliafigo Spreng%含笑花精油的抑菌活性及其化学成分分析

    Institute of Scientific and Technical Information of China (English)

    杨波华; 马英姿; 杨蕾; 张慧; 宋荣

    2011-01-01

    运用水蒸气蒸馏法提取含笑花精油,分别采用平板菌落法和滤纸片法检测精油对细菌和真菌的抑菌活性,并运用GC/MS法分析精油的化学成分.结果表明:含笑花精油对枯草芽孢杆菌、大肠杆菌和总状毛霉抑制效果较佳,抑菌率分别达70.36%、60.24%和65.52%;对金黄色葡萄球菌、黄曲霉具有一定的抑制作用,对米根霉无抑制效果;从含笑花精油中分离鉴定出30种化合物,其中脂肪族化合物14种、萜烯类化合物16种.%Essential oil from the flowers of Michelia figo was extracted by steam distillation and the antimicrobial activities of the essential oil on bacteria and fungi were tested by plate counts and filter paper methods.Then the chemical constituents of the essential oil were analyzed by GC/MS.The results showed that: the antimicrobial effect of the essential oil from the flowers of M.figo on Bacillus sabtills, Escherichia coli and Mucor racemosus were good and their inhibitory rates were up to 70.36%, 60.24% and 65.52%.There were certain antimicrobial effect of the essential oil on Staphylococcus aureus and Aspergillusfiavus, but no antimicrobial effect on Rhizopus oryzae.Thirty compounds were separated and identified from the essential oil including 14 aliphatic compounds and 16 terpenoid compounds.

  16. Chemotaxonomic significance of flavonoids, coumarins and triterpenes of Augusta longifolia (Spreng. Rehder, Rubiaceae-Ixoroideae, with new insights about its systematic position within the family Significância quimiotaxômica de flavonoides, cumarinas e triterpenos de Augusta longifolia (Spreng. Rehder, Rubiaceae-Ixoroideae, com novos entendimentos sobre a posição sistemática dentro da família

    Directory of Open Access Journals (Sweden)

    Rafael Choze

    2010-07-01

    Full Text Available Augusta has traditionally been placed in the tribe Rondeletieae, subfamily Cinchonoideae. However, recent molecular phylogenies positioned it near to Wendlandia (Ixoroideae, but locate A. longifolia near to the clade Ixoroidinae II. The study of A. longifolia afforded two coumarins, five flavonoids, three triterpenoids and one benzoic acid derivative. These metabolites reinforce the separation of Augusta as a monospecific genus, and Lindenia as a genus of three species, closely related to Wendlandia.Augusta tem sido tradicionalmente colocada na tribo Rondeletieae, Cinchonoideae subfamília. No entanto, recentes filogenias moleculares posicionou-a perto de Wendlandia, porém localizando A. longifolia perto do clado Ixoroidinae II. O estudo de A. longifolia resultou em duas cumarinas, cinco flavonoides, três triterpenoides e um derivado do ácido benzóico. Estes metabolitos reforçam a separação da Augusta como um gênero monoespecífico, e Lindenia como um gênero de três espécies, intimamente relacionada com Wendlandia.

  17. Redução de aminoácidos em polpas de bacuri (Platonia insignis Mart, cupuaçu (Theobroma grandiflorum Willd ex-Spreng Schum e murici (Byrsonima crassifolia L. processado (aquecido e alcalinizado Amino acids reduction in processed (heated and alkalinized pulps of bacuri (Platonia insignis Mart, cupuaçu (Theobroma grandiflorum Willd ex-Spreng Schum and murici (Byrsonima crassifolia L.

    Directory of Open Access Journals (Sweden)

    Alexandre Porte

    2010-09-01

    Full Text Available A literatura científica é pobre a respeito de frutas da Amazônia, como o murici, e suas características químicas devem ser estudadas. Por isso, esta pesquisa teve por proposta determinar o perfil aminoacídico das polpas de bacuri, cupuaçu e murici sob diferentes valores de pH (3,3, 5,8, 8,0 e 12,0, sem aquecimento ou com aquecimento por 12 horas/100 ºC com agitação e refluxo. Valores de pH, glicose, frutose e sacarose também foram determinados nas polpas sem aquecimento. Os nutrientes foram determinados por CLAE (Cromatografia Líquida de Alta Eficiência. As polpas de bacuri, cupuaçu e murici apresentaram valores de pH 3,2, 3,6 e 3,35, respectivamente. A sacarose foi, quantitativamente, o principal carboidrato nas polpas de cupuaçu (38,34% e bacuri (36,93%, sendo que os teores de frutose e glicose foram similares, tanto na polpa de cupuaçu (8,93% e 9,03% como na de bacuri (12,63% e 11,65%, respectivamente. Em contraste, a polpa de murici foi quase isenta de sacarose (0,57%, mas não de frutose (11,51% ou glicose (11,39%. Nas polpas sem aquecimento, os principais aminoácidos foram: ácido glutâmico (46,6 mg/kg, ácido aspártico (28,8 mg/kg e arginina (25,3 mg/kg na polpa de bacuri; ácido aspártico (56,3 mg/kg, ácido glutâmico (44,0 mg/kg e alanina (24,2 mg/kg na polpa de cupuaçu; prolina (73,5 mg/kg, ácido glutâmico (23,7 mg/kg e ácido aspártico (23,5 mg/kg na polpa de murici. O aquecimento reduziu as concentrações de todos os aminoácidos nas 3 polpas. O meio fortemente alcalino (pH 12 produziu a maior degradação de aminoácidos. Lisina foi mais sensível ao aquecimento do que outros aminoácidos em pH 12.Scientific literature presents few studies about fruits of the Amazonia, like murici, and yours chemical characteristics should be studied. Therefore, amino acid profiles of the bacuri, cupuaçu and murici pulps were determined under different values of pH (3.3, 5.8, 8.0 and 12.0 with heating (12 hours/100 ºC, with stirring and refluxing or without heating. Glucose, fructose, sucrose and pH values also were obtained in the pulps without heating. All nutrients were analised by HPLC. The pHs were: 3.2, 3.6 and 3.35 in the bacuri, cupuaçu and murici pulps, respectively. Sucrose (38.34% and 36.93% was the major carbohydrate while fructose (8.93% and 12.63% and glucose (9.03% and 11.65% shown similar percentages in the cupuaçu and bacuri pulps. Murici pulp was almost free of sucrose (0.57%, but not of fructose (11.51% or glucose (11.39%. In the pulps without heating the major amino acids were: glutamic acid (46.6 mg/kg, aspartic acid (28.8 mg/kg and arginine (25.3 mg/kg in the bacuri pulp; aspartic acid (56.3 mg/kg, glutamic acid (44.0 mg/kg and alanine (24.2 mg/kg in the cupuaçu pulp; proline (73.5 mg/kg, glutamic acid (23.7 mg/kg and aspartic acid (23.5 mg/kg in the murici pulp. The heating of the 3 pulps decresead the concentration of all amino acids. The medium strongly alkaline (pH 12 produced more degradation of the amino acids than others pHs. Lysine was more sensible to the heating than others amino acids in pH 12.

  18. MonoTrap法捕集白木香愈伤组织挥发性成分及其GC-MS分析%GC-MS Analysis on the Volatile Constituents Adsorbed with Mono Trap from Callus of Aquilaria sinensis (Lour.) Gilg

    Institute of Scientific and Technical Information of China (English)

    张珂; 陈华姑; 龚胜; 孙海燕; 王健

    2015-01-01

    采用新型材料MonoTrap作为顶空固相萃取的吸附剂,捕集茉莉酸甲酯(MeJA)处理后的白木香愈伤组织挥发性成分,并应用GC-MS分析测定其化学成分及相对含量.结果表明,从MeJA不同处理下的白木香愈伤组织中共鉴定出56种挥发物成分,主要成分为脂肪烃类、芳香族类和倍半萜类等成分;通过正交实验法确定最佳捕集方法为:不破碎愈伤组织材料,于30℃条件下、采用MonoTrap捕集6h.MonoTrap法结合气质联用具有高灵敏度、强针对性和可操作性强的优点,可以为白木香的开发利用提供理论依据.

  19. Study on the Polyploid Induction of Melastoma dodecandrum Lour. by Using Colchicine%利用秋水仙素诱导地菍多倍体的研究

    Institute of Scientific and Technical Information of China (English)

    胡松梅; 蒋道松; 龚泽修

    2008-01-01

    [目的]探索利用秋水仙素诱导地蒸多倍体的可行性.[方法]以秋水仙素作为诱变剂,对地菍无菌试管苗丛芽进行多倍体诱导,研究秋水仙素浓度、处理方法和处理时间对地菍组培苗丛芽诱导效果的影响.经纯化和鉴定后,分析地菍多倍体的外部形态和叶片气孔特征.[结果]采用混培法用0.1%秋水仙素对地菍处理14 d的诱导率达28%,明显优于浸泡法.秋水仙素浓度对地菍染色体的加倍率影响显著.采用不定芽技术切取变异株顶芽继代5次后,可得到纯合四倍体地菍植株,染色体数目为2n=56.与正常二倍体相比,四倍体地菍植株表现出多倍体巨大型特征.[结论]采用混培法用0.1%秋水仙素处理地菍试管苗丛芽21 d,可获得最好的诱导效果.

  20. Research on the acute toxicity and genetic toxicity of Aquilaria sinensis (Lour.) Gilg.leaf%沉香叶的急性毒性和遗传毒性研究

    Institute of Scientific and Technical Information of China (English)

    李庆; 黄俊明; 杨颖; 陈美芬; 梁扬盛; 陈秀娟

    2015-01-01

    目的 对沉香叶的急性经口毒性、遗传毒性进行研究,为沉香叶的开发和应用提供毒理学安全性实验依据.方法 按照GB 15193-2003对沉香叶进行小鼠经口急性毒性试验(Horn’s法,设21.5 g/kg体重、10.0 g/kg体重、4.64 g/kg体重、2.15 g/kg体重4个剂量组)、小鼠精子畸形试验(设10.0 g/kg体重、5.00 g/kg体重、2.50 g/kg体重3个剂量组及阴性、阳性对照组)、小鼠骨髓细胞微核试验(设10.0 g/kg体重、5.00 g/kg体重、2.50 g/kg体重3个剂量组及阴性、阳性对照组)及鼠伤寒沙门菌回复突变试验(设5 000 μg/皿、1 000 μg/皿、200 μg/皿、40μg/皿、8μg/皿5个剂量组,同时设自发回变、溶剂对照和阳性对照组).结果 沉香叶对小鼠的急性经口LD50 >21.5 g/kg体重;各剂量组微核率、精子畸形率、回复突变数和阴性对照组比较均无显著性增加;3项毒理学遗传试验结果均为阴性.结论 在本实验条件下,沉香叶属于无毒级物质,未见有遗传毒性作用.

  1. 龙眼胚性愈伤组织中TFL1基因克隆及其表达分析%Cloning and Characterization of TFL1 Family Genes from Embryogenic Callus in Dimocarpus longan Lour.

    Institute of Scientific and Technical Information of China (English)

    2015-01-01

    TFL1 is belonging to the FT/TFL1 family, and it is involved in delaying the plant flowering time through maintaining the apical meristem. The RT-PCR combined with RACE method was used to obtain the complete cDNA and gDNA sequences of DlTFL1 from embryogenic callus in Dimocarpus longan. The complete cDNA sequence of DlTFL1 was 866 bp, encoding 175 amino acids. The gDNA sequence of DlTFL1 was 1 492 bp, contains three introns and for exons. DlTFL1 was stable and hydrophilic proteins, without signal peptides and Tran membrane structures, had the PEBP protein function domain. Phylogenetic tree analyses indicated that DlTFL1 has a close genetic relationship with Jatropha curca、Vitis vinifera、Populus trichocarpa. qPCR result indicated that the expressing of DlTFL1 was very differential in“Sijimi” different tissues, it expressed at the highest level in the flower bud, lower in root, leaf and flower, but not expressing in other tissues. DlTFL1 is a plant TFL1 homologous gene, may have inhibitory effect on flowering, it may promote the development of early flowers of“Sijimi”longan.%TFL1是FT/TFL1家族的一个成员,TFL1通过对顶端分生组织的维持从而延迟了植物开花时间。以龙眼松散型胚性愈伤组织为材料,采用RT-PCR结合RACE法克隆了TFL1的cDNA全长和gDNA序列,命名为DlTFL1。 DlTFL1全长866 bp,编码175个氨基酸。 DlTFL1的gDNA序列长为1492 bp,由3个内含子和4个外显子组成。 DlTFL1为稳定的亲水蛋白,没有信号肽与跨膜结构,含有PEBP功能保守结构域。进化树分析表明, DlTFL1与小油桐、葡萄、毛果杨等植物TFL1的亲缘关系较近。 q-PCR表明,DlTFL1在“四季蜜”龙眼不同组织部位中的表达差异明显,在花蕾中的表达量最高,在根、叶和花中表达量较低,在其他部位甚至不表达。说明DlTFL1是植物TFL1同源基因,可能具有抑制开花作用,对“四季蜜”龙眼花早期的器官发育可能有促进作用。

  2. UPTAKE OF THE CYANOBACTERIAL HEPATOTOXIN MICROCYSTIN-RR BY SUBMERGENT MACROPHYTE VALLISNERIA NATANS(LOUR.)HARA.%微囊藻毒素在沉水植物苦草中的积累

    Institute of Scientific and Technical Information of China (English)

    尹黎燕; 黄家权; 沈强; 李敦海; 刘永定

    2004-01-01

    研究了微囊藻毒素RR在大型沉水植物苦草根、叶组织中的积累作用,通过ELISA方法检测发现,苦草可以吸收MC-RR,其吸收具有时间和剂量效应,根的吸收作用强于叶.第7d叶对MC-RR的吸收已经达到平衡,而根对MC-RR的吸收在处理第16d时还在上升.苦草根和叶对MC-RR的最大吸收量分别可达到14.83±0.12μg/g.FW和0.32±0.026μg/g.FW.在0.0001mg/L的低浓度下,苦草根和叶对MC-RR的吸收量分别为0.59±0.083pg/g.FW和0.28±0.016pg/g.FW,生物浓缩系数分别为5.85±0.83和2.85±0.16.这个结果表明,MC-RR可以在苦草中积累并有可能转移到食物链中.

  3. 植物染料に関する研究(第 2 報) : ソメモノイモ (Dioscorea chirrhosa L_>) の染色性(林学科)

    OpenAIRE

    屋我, 嗣良; 太田, 薫; 金城, 一彦; Yaga, Shiryo; Oota, Kaoru; Kinjo, Kazuhiko

    1989-01-01

    ソメモノイモの染色性について検討し, 次の結果を得た。1)ソメモノイモの熱水抽出物は約17%であった。2)素材に絹布を用いて染色条件を検討した。その結果, 最適染色条件は染料濃度が5%, 染色温度が80℃, および染色時間は60分であった。3)最適染色条件で染色した絹布を重クロム酸カリウム, 及び硫酸第一鉄で媒染を行ったとき発色が顕著だった。また, この時の媒染色剤濃度は, 重クロム酸カリウムで75ppm, 硫酸第一鉄で100ppmが適当と考えられた。4)染色堅牢度のうち最も重要な日光堅牢度, および洗濯堅牢度について検討した。最適染色条件で染色し, ここでは, 重クロム酸カリウム, および硫酸第一鉄で媒染した。その結果, 日光堅牢度が優れており(5級以上), また洗濯堅牢度も5回以上の重ね染めにより安定した。...

  4. Extraction of Water-Soluble Pigment from Fruit Peels of Ligustrum sinense Lour. and Its Light Sensitivity%小蜡果皮水溶性色素的提取及光敏感性

    Institute of Scientific and Technical Information of China (English)

    储敏

    2006-01-01

    以去离子水和不同体积分数乙醇从小蜡果皮中浸提红色素,用光谱扫描法检测该红色素的光吸收特性,比较不同浸提时间及不同体积分数乙醇对浸提效果的影响,并用暴露方式进行光敏感性测定.结果显示,小蜡果皮色素在纯水中的溶解性最好,属水溶性色素,延长浸提时间可提高色素的浸出率,但杂质质量分数也随之提高,紫外线对色素的色度影响最大,直接照射可使色素的色度大幅度下降.

  5. Carbon Fixation and Oxygen Release of 18 Shrub Greening Trees%18种常见灌木绿化树种光合特性及固碳释氧能力分析

    Institute of Scientific and Technical Information of China (English)

    林欣; 林晨菲; 刘素青; 李林锋

    2014-01-01

    14 00 and 16 00 in the afternoon. The capacity of carbon fixation and oxygen release among the shrub species are also different. The four high carbon fixation and oxygen release species are Lantana camara L., Duranta repens L., Ficusmicrocarpa cv. Golden Leaves and Acalypha wilkesiana Muell.-Arg., and the weakest plant was Rhapis excelsa Thunb. Based on the net amount of carbon storage, the shrub trees were divided trees into three categories by using the cluster analysis. The high daily carbon storage and oxygen release group including Lantana camara L., Duranta repens L., Ficusmicrocarpa cv. Golden Leaves and Acalypha wilkesiana Muell.-Arg.[7~10 g/(m2·d) and 5~7 g/(m2·d)], medium daily carbon storage and oxygen release group including Bougainvillea spectabilis Willd., Hibiscus rosa-sinensis L., Murraya exotica L., Aglaia odorata Lour., Schefflera arboricola H., Michelia figo (Lour.) Spreng., Ervatamia divaricata (L.) Burk. cv. Gouyahua, the low daily carbon storage and oxygen release group Cordyline fruticosa (L.) A. Cheval., Codiaeum variegatum (L.) A. Juss., Excoecaria cochinchinensis Lour., Jasminum sambac (L.) Ait., Phoenix roebelenii O. Brien, Carmona microphylla (Lam.)G. Don and Rhapis excelsa (Thunb.) Henry ex Rehd. The study will provide the certain reference for greening tree species selection in order to meet carbon forestry.

  6. Performance of resistance gene pyramids to races of rice bacterial blight in Zhejiang Province

    Institute of Scientific and Technical Information of China (English)

    ZHENGKangle; ZHUANGJieyun; WANGHanrong

    1998-01-01

    The effect of gene pyramiding on resistance to bacterial blight (BB) in rice was evahlated among the IR24-based near isogenic lines conraining single resistance gene and gene pyramids containing two, three or lour resistancegenes (see table).

  7. Variation of karyotype and nuclear DNA content among four species of Plectranthus L’ Héritier, 1788 (Lamiaceae) from Brazil

    Science.gov (United States)

    Nani, Thaís Furtado; Mesquita, Amanda Teixeira; Bustamante, Fernanda de Oliveira; Barbosa, Sandro; Barbosa, João Vítor Calvelli; Davide, Lisete Chamma

    2015-01-01

    Abstract Plectranthus is a genus which includes species of ornamental and medicinal potential. It faces taxonomic problems due to aggregating species previously belonging to the genus Coleus, a fact that has contributed to the existence of various synonymies. The species Plectranthus amboinicus, Plectranthus barbatus, Plectranthus grandis and Plectranthus neochilus are included in this context. Some authors consider Plectranthus barbatus and Plectranthus grandis as synonyms. The present work was carried out with the aim of comparing plants of the above-mentioned species, originating from different localities in Brazil, with regards to chromosome number and karyotypic morphology, correlated to the nuclear DNA content. There was no variation in chromosome number among plants of the same species. Plectranthus amboinicus was the only species to exhibit 2n=34, whereas the others had 2n=30. No karyotypic differences were found among the plants of each species, except for Plectranthus barbatus. The plants of the Plectranthus species revealed little coincidence between chromosome pairs. The nuclear DNA content allowed grouping Plectranthus amboinicus and Plectranthus neochilus, with the highest mean values, and Plectranthus grandis and Plectranthus barbatus with the lowest ones. Differences in DNA amount among the plants were identified only for Plectranthus barbatus. These results allow the inference that the populations of Plectranthus amboinicus and Plectranthus neochilus present coincident karyotypes among their plants, and Plectranthus grandis is probably a synonym of Plectranthus barbatus. PMID:26753074

  8. Study of the Interference between Plectranthus Species Essential Oils from Brazil and Aminoglycosides

    Directory of Open Access Journals (Sweden)

    Fabíola Fernandes Galvão Rodrigues

    2013-01-01

    Full Text Available Plectranthus is one of the most representative genera of Lamiaceae family. In this study, the essential oils from Plectranthus amboinicus, Plectranthus ornatus, and Plectranthus barbatus were investigated for their chemical composition and antimicrobial and modulatory activities. The major components found were carvacrol (54.4%—P. amboinicus and eugenol (22.9%—P. ornatus e 25.1%—P. barbatus. In vitro antimicrobial activity was conducted against Escherichia coli, Proteus vulgaris, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus aureus (multiresistant using microdilution method. The results of bioassay showed that all strains were sensitive to the oils, except P. aeruginosa that was resistant to P. amboinicus and P. ornatus. A synergistic effect of all essential oils combined with the aminoglycosides was demonstrated. These results show that P. amboinicus, P. ornatus, and P. barbatus inhibit the growth of pathogenic microorganism, and besides this they present antibiotic modifying activity, providing a new perspective against the problem of bacterial resistance to antibiotics.

  9. Variation of karyotype and nuclear DNA content among four species of Plectranthus L' Héritier, 1788 (Lamiaceae) from Brazil.

    Science.gov (United States)

    Nani, Thaís Furtado; Mesquita, Amanda Teixeira; Bustamante, Fernanda de Oliveira; Barbosa, Sandro; Barbosa, João Vítor Calvelli; Davide, Lisete Chamma

    2015-01-01

    Plectranthus is a genus which includes species of ornamental and medicinal potential. It faces taxonomic problems due to aggregating species previously belonging to the genus Coleus, a fact that has contributed to the existence of various synonymies. The species Plectranthus amboinicus, Plectranthus barbatus, Plectranthus grandis and Plectranthus neochilus are included in this context. Some authors consider Plectranthus barbatus and Plectranthus grandis as synonyms. The present work was carried out with the aim of comparing plants of the above-mentioned species, originating from different localities in Brazil, with regards to chromosome number and karyotypic morphology, correlated to the nuclear DNA content. There was no variation in chromosome number among plants of the same species. Plectranthus amboinicus was the only species to exhibit 2n=34, whereas the others had 2n=30. No karyotypic differences were found among the plants of each species, except for Plectranthus barbatus. The plants of the Plectranthus species revealed little coincidence between chromosome pairs. The nuclear DNA content allowed grouping Plectranthus amboinicus and Plectranthus neochilus, with the highest mean values, and Plectranthus grandis and Plectranthus barbatus with the lowest ones. Differences in DNA amount among the plants were identified only for Plectranthus barbatus. These results allow the inference that the populations of Plectranthus amboinicus and Plectranthus neochilus present coincident karyotypes among their plants, and Plectranthus grandis is probably a synonym of Plectranthus barbatus. PMID:26753074

  10. 沙蒿散缓解阻生牙拔除术后日常生活活动能力影响因素的效果评价%Effect evaluation of artemisia desertorum spreng on ability of daily life after extraction of impacted tooth

    Institute of Scientific and Technical Information of China (English)

    史亮; 胡开进; 于擘; 彭莲

    2003-01-01

    AIM:To investigate the effect of locally used Shahaosan on preventing the complication of extraction of impacted tooth.METHODS: 274 cases patients with extraction of impacted teeth were randomly devided into 3 groups named A,B and C.Shahaosan and Yunnan white drug were processed into drug A and B by department of pharmacy with the same color, shape and quality which are blind to doctors and patients.When the impacted teeth were extracted, administed group A(92 cases) with drug A,group B(86 cases) with drug B and group C(96 cases ) was a blank control. After the operation,incidence and severe intensity of dry socket in each group were observed and evaluated by scores.RESULTS: The incidence of dry socket in group A,B and C were 0.09% (1/92),2.32% (2/86),8.33% (8/96).There was no significant difference between group A and B, group B and C, while a significant difference showed between group A and C.The PoSSe score in group A, B and C was 19.36, 27.80 and 22.83 respectively.Afer the experiment the department of pharmacy informed that drug A is Shahaosan and drug B is Yunnan white drug.CONCLUSTON:Compiments of dry socket induced by extracting of lower jaw impacted teeth can be prevented by locally administered with Shahaosan, but no significant effect of alleviating the symptom of dental extraction.

  11. Effects of Stand Types on the Community Diversity of Ground-Dwelling Beetles in the Invaded Regions of Eupatorium adenophorum Spreng.%林分类型对西昌紫茎泽兰入侵地地表甲虫群落的影响

    Institute of Scientific and Technical Information of China (English)

    亓东明

    2013-01-01

    The diversity of ground-dwelling beetles in 5 stand types from the suburbs of Xichang city was investigated with the bait-traps method.886 beetles' specimens were gathered and belonged to 15 families.The Geotrupidae,Rutelidae and Staphylinidae were the dominant groups whose individuals were the most.The number of individuals of ground-dwelling beetles was the highest in the Pinus yunnanensis forest among the five stand types (P<0.01).The study of diversity showed that the richness of the ground-dwelling in Pinus yunnanensis forest and the Cupressus funebris forest were significantly higher than that of the Quercus acutissima forest(P<0.05).The dominance and evenness of the 5 stand types had no significant difference.The diversity of the Pinus yunnanensis forest had very significant difference with the Abies sp.forest and Dodonaea viscose shrub (P<0.01).The study of similarity showed that most of the similarity was middling dissimilar and middling similar,but the similarity of the Abies sp.forest and shurb was significantly different.The results showed that the composition and individuals of the ground-dwelling beetles in the 5 stand types of the suburbs of Xichang city had significant difference with the natural pure forest.It was suggested to design stand rebuilding for the artificial pure forest to increase the biodiversity.%2010年7~9月主要采用巴氏罐诱法对四川西昌市郊紫茎泽兰入侵地5种类型林分的地表甲虫群落进行调查,共采集地表甲虫标本886份,隶属15科,其中粪金龟科、隐翅虫科和丽金龟科昆虫个体数量多,是西昌市郊林下地表甲虫的优势类群.云南松(Pinus yunnanensis)林林下地表甲虫个体数量极显著高于其他4种林分(P<0.01).多样性分析表明,云南松林与柏木(Cupressus funebris)林林下地表甲虫丰富度指数显著高于麻栎(Quercus acutissima)林(P<0.05).各林分类型地表甲虫优势度指数及均匀度指数差异不显著,柏木林优势度指数、均匀度指数最高.云南松林与冷杉(Abies sp.)林、车桑子(Dodonaea viscose)灌丛林林下地表甲虫多样性指数差异极显著(P<0.01).相似性研究表明,不同林分类型地表甲虫群落相似性多为中等不相似及中等相似,仅冷杉林和车桑子灌丛林为极不相似.研究结果表明,西昌市郊各类型林分地表甲虫组成和数量分布与天然林存在一定差异,建议对人工纯林进行林分改造以增加生物多样性.

  12. Microcal Orimetric Study of Bacteriostasic Activity of Alkaloids of Chinese Drugs-lotus Leaf and Fibraurea Tinctoria Lour on the Growth of Escherichia%荷叶和黄藤中的生物碱对大肠杆菌代谢作用的微量量热法研究

    Institute of Scientific and Technical Information of China (English)

    王建涛; 杜丽敏; 张洪林

    2006-01-01

    从荷叶、黄藤中提取其中的生物碱,用瑞典产的2277型热活性检测仪测定了37℃下不同浓度的生物碱对大肠杆菌代谢作用的热功率-时间曲线,计算出细菌的生长速率常数,建立了生长速率常数与药物浓度之间的关系,进而确定了最佳用药浓度.

  13. 棕榈科麒麟血竭与百合科剑叶血竭HPLC指纹图谱分析与比较%Comparison Daemonorops Draco Bl.with Dracaena Cochinchinensis (Lour.) S.C.Chen by HPLC Fingerprint Analysis

    Institute of Scientific and Technical Information of China (English)

    黄茂华; 雍克岚

    2001-01-01

    采用反相高效液相色谱法,首次建立了进口棕榈科麒麟血竭和国产百合科剑叶血竭HPLC指纹图谱,运用相对保留指纹谱的方法,对获得的图谱进行数据化处理,得出重叠率、n强峰、特征峰群和特征指纹峰检出率等参数,从中找出不同血竭品种所含化合物之间的相互关系及特征.结果表明,进口血竭与国产血竭的HPLC指纹图谱差异明显,同一品种不同产地的国产血竭指纹谱极为相似,但主成份含量有一定的差别.

  14. Los géneros Cajanus y Rhynchosia (Leguminosae, Papilionoideae, Phaseoleae, Cajaninae) en la península de Yucatán, México The genera Cajanus and Rhynchosia (Leguminosae, Papilionoideae, Phaseoleae, Cajaninae) in the Yucatán Peninsula, Mexico

    OpenAIRE

    Angélica Martínez-Bernal; Rodrigo Duno-de Stefano; Lilia Lorena-Can

    2011-01-01

    Se presenta información general de los géneros Cajanus DC. y Rhynchosia Lour. (subfamilia Papilionoideae, tribu Phaseoleae, subtribu Cajaninae) en la península de Yucatán. En esta región, Cajanus incluye 1 especie introducida y Rhynchosia incluye 6 nativas. Se presentan claves para géneros y especies, información ecológica, fenológica, nombres comunes y usos de cada especie.An overview of the genera Cajanus DC. and Rhynchosia Lour. (subfamily Papilionoideae, tribe Phaseoleae, subtribe Cajanin...

  15. Pulse diversity for polyphenolic secondary metabolites

    Science.gov (United States)

    The pulse species including guar (Cyamopsis tetragonoloba L. Taub.), Lablab purpureus L. Sweet, Macrotyloma uniflorum (Lam.) Verdc., Teramnus labialis (L.f.) Spreng, alfalfa (Medicago sativa ssp. sativa), green beans (Phaseolus vulgaris L.), fava beans (Vicia faba L.), blackeye pea (Vigna unguiculat...

  16. Review of traditional and non-traditional medicinal genetic resources in the USDA, ARS, PGRCU collection evaluated for flavonoid concentrations and anthocyanin indexes

    Science.gov (United States)

    Non-traditional medicinal species include velvetleaf (Abutilon theophrasti Medik.), Desmodium species, Termanus labialis (L.f.) Spreng. and the traditional species consists of roselle (Hibiscus sabdariffa L.). There is a need to identify plant sources of flavonoids and anthocyanins since they have s...

  17. Maailmakuulsa Marc Chagalli litograafiad jõudsid Pärnusse / Ants Juske

    Index Scriptorium Estoniae

    Juske, Ants, 1956-

    1999-01-01

    60. aastatel tehtud litograafiad on pärit Hannoveri Sprengeli muuseumist. Pärnu Uue Kunsti Muuseumis näitusel 'Testament' on need eksponeeritud koos Herman Talviku graafikaga, mis on pärit Rootsis elava Harald Keilandi (Ilmar Malini vend) kogust

  18. Enkele nieuwe vondsten van Cladonia’s in Nederland

    NARCIS (Netherlands)

    Masselink, A.K.; Sipman, H.J.M.

    1985-01-01

    Some interesting records of lichens belonging to the genus Cladonia Wigg. are reported. Cladonia cariosa (Ach.) Spreng. and C. fragilissima Østh. & P. James have recently been found for the first time in the Netherlands. C. symphycarpa (Ach.) Fr., hitherto only collected once, has been discovered on

  19. Antileishmanial activity of piceatannol isolated from Euphorbia lagascae seeds

    NARCIS (Netherlands)

    Duarte, Noelia; Kayser, Oliver; Abreu, Pedro; Ferreira, Maria-Jose U.

    2008-01-01

    In the search for biologically active compounds from Euphorbia lagascae Spreng, an herbaceous plant native to southeast of Iberic Peninsula, a stilbene, two coumarins and two 1-2-deoxyphorbol diterpene esters were isolated by chromatographic methods, from the methanol extracts of its defatted seeds.

  20. Chromosome studies on African plants. 11. The tribe Andropogoneae (Poaceae: Panicoideae

    Directory of Open Access Journals (Sweden)

    J. J. Spies

    1994-12-01

    Full Text Available Representative specimens of various species of the genera  Andropogon L.,  Cymbopogon Spreng.,  Elionurus Kunth ex Willd.,  Hyparrhenia Foum. and  Hyperthelia Clayton were cytogenetically studied. All specimens had a secondary basic chromosome number of ten. Polyploidy, either as alloploidy or segmental alloploidy. was frequent. The taxa studied represent mature polyploid complexes.  

  1. Lantana montevidensis Essential Oil: Chemical Composition and Mosquito Repellent Activity against Aedes aegypti

    Science.gov (United States)

    The essential oil (EO) of Lantana montevidensis (Spreng.) Briq. (L. sellowiana Link & Otto) was investigated for its chemical composition and mosquito repellent activity. The essential oil obtained by hydrodistillation of aerial plant parts was analyzed by GC-FID and GC-MS. The major constituents we...

  2. The application of HPLC ESI MS in the investigation of the flavonoids and flavonoid glycosides of a Caribbean Lamiaceae plant with potential for bioaccumulation.

    Science.gov (United States)

    Peter, Sonia R; Peru, Kerry M; Fahlman, Brian; McMartin, Dena W; Headley, John V

    2015-01-01

    As part of an exchange technology program between the government of Barbados and Environment Canada, methanolic and aqueous extracts from the flavonoid-rich Lamiaceae family were characterized using negative-ion electrospray mass spectrometry. The species investigated is part of the Caribbean Pharmacopoeia, and is used for a variety of health issues, including colds, flu, diabetes, and hypertension. The extracts were investigated for structural elucidation of phenolics, identification of chemical taxonomic profile, and evidence of bio-accumulator potential. The methanolic and aqueous leaf extracts of Plectranthus amboinicus yielded rosmarinic acid, ladanein, cirsimaritin, and other methoxylated flavonoids. This genus also shows a tendency to form conjugates with monosaccharides, including glucose, galactose, and rhamnose. The aqueous extract yielded four isomeric rhamnosides. The formation of conjugates by Plectranthus amboinicus is thus evidence of high bioaccumulator significance. PMID:26357892

  3. Study on Comprehensive Effect of Grafting Siraitia grosvenorii on Cucurbitaceae Plants%葫芦科植物嫁接罗汉果的综合效应研究

    Institute of Scientific and Technical Information of China (English)

    薛进军; 秦忠明

    2013-01-01

    采用葫芦科植物栝楼、葫芦、木鳖作为砧木嫁接罗汉果,以罗汉果本砧和组培苗作为对照,探讨了不同砧穗组合对罗汉果生长与结果等方面的综合效应.结果表明,葫芦科植物科内嫁接罗汉果具有良好的亲和性;用葫芦做砧木的罗汉果嫁接植株与罗汉果本砧相比,具有结果多、产量高、品质好、抗根结线虫能力较强的特点,而且嫁接操作比较容易;木鳖根系强大,抗根结线虫能力强,嫁接罗汉果成活率最高,植株生长旺盛,但是嫁接植株没有开花,可以考虑做基砧用;栝楼种子发芽比较困难,通过变温催芽处理(25 ℃/12 h、35℃/12 h)可提高发芽率,做砧木嫁接罗汉果亲和性一般,坐果率高,高抗根结线虫,但产量和品质不如葫芦砧和罗汉果组培苗,可以作为备选砧木考虑;罗汉果本砧和组培苗易发生根结线虫病,但是组培苗的果实产量和品质较好.在嫁接方法选择上,劈接法嫁接的成活率高于顶插法,而且在株高、茎粗、叶片数和叶片大小等幼苗生长能力方面都显著高于顶插法;在嫁接苗的管理上,于园土中加入20%蚯蚓粪的混合基质能显著促进罗汉果嫁接苗的生长.%Cucurbitaceae plants including Trichosanthes kirilowii Maxim,Lagenaria siceraria (Molina) Standl.,Momordica cochinchinensis (Lour.)Spreng.were used as grafting rootstock of Siraitia grosvenorii (Swingle) C.Jeffrey ex Lu et Z.Y.Zhang,taking S.grosvenorii tissue culture seedlings and S.grosvenorii raw plant as rootstock as control to explore the comprehensive effects of different combinations of scion on the growth and fruiting of S.grosvenorii.Results showed that the Cucurbitaceae plants and S.grosvenorii had good grafting compatibility.Compared to S.grosvenorii tissue culture seedlings and S.grosvenorii raw plant as rootstock,using L.siceraria as rootstock,the S.grosvenorii was with more fruits,high yield,good quality,strong Meloidogyne

  4. Genetic similarities among four species of the Plectranthus (L’Hér.) genus - DOI: 10.4025/actascibiolsci.v32i1.880 Genetic similarity among four species of Plectranthus genus - DOI: 10.4025/actascibiolsci.v32i1.880

    OpenAIRE

    Eugenia Jacira Bolacel Braga; José Antonio Peters; Valmor João Bianchi; Sílvia Rubin; Juliana de Magalhães Bandeira

    2009-01-01

    The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus)...

  5. Genetic similarities among four species of the Plectranthus (L’Hér.) genus = Similaridade genética de quatro espécies do gênero Plectranthus

    OpenAIRE

    Juliana de Magalhães Bandeira; Valmor João Bianchi; Silvia Rubin; José Antonio Peters; Eugenia Jacira Bolacel Braga

    2010-01-01

    The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus)...

  6. Variation of karyotype and nuclear DNA content among four species of Plectranthus L’ Héritier, 1788 ( Lamiaceae ) from Brazil

    OpenAIRE

    Nani, Thaís Furtado; Mesquita, Amanda Teixeira; Bustamante, Fernanda de Oliveira; Barbosa,Sandro; Barbosa, João Vítor Calvelli; Davide, Lisete Chamma, 1953-

    2015-01-01

    Abstract Plectranthus is a genus which includes species of ornamental and medicinal potential. It faces taxonomic problems due to aggregating species previously belonging to the genus Coleus , a fact that has contributed to the existence of various synonymies. The species Plectranthus amboinicus , Plectranthus barbatus , Plectranthus grandis and Plectranthus neochilus are included in this context. Some authors consider Plectranthus barbatus and Plectranthus grandis as synonyms. The present wo...

  7. Toxicity of essential oil from Indian borage on the larvae of the African malaria vector mosquito, Anopheles gambiae

    OpenAIRE

    Kweka Eliningaya J; Senthilkumar Annadurai; Venkatesalu Venugopalan

    2012-01-01

    Abstract Background Essential oils are currently studied for the control of different disease vectors, because of their efficacy on targeted organisms. In the present investigation, the larvicidal potential of essential oil extracted from Indian borage (Plectranthus amboinicus) was studied against the African anthropophagic malaria vector mosquito, Anopheles gambiae. The larvae of An. gambiae s.s laboratory colony and An. gambiae s.l of wild populations were assayed and the larval mortality w...

  8. Screening and antibacterial activity analysis of some important medicinal plants

    Directory of Open Access Journals (Sweden)

    G. Senthilmurugan Viji

    2013-02-01

    Full Text Available The screening and study of five different plant specimens belonging to different families for phytochemical constituents was performed using generally accepted laboratory technique for qualitative determinations. The constituents screened were saponins, combined anthraquinones, terpenoids, flavonoids, carotenoids, steroids, xantho proteins, couramins, alkaloids, quinones, vitamin C. The distribution of these constituents in the plant specimens were assessed and compared. The medicinal plant studied were Acalypha indica, Camellia sinensis, Plectranthus amboinicus, Curcuma longa, Rauvolfia tetraphylla. All the plant speciemens were found to contain terpenoids, xantho proteins, couramins and vitamin C. They also contain Saponins (except Curcuma longa, Combined anthroquinones (except Acalypha indica, Camellia sinensis, Curcuma longa flavonoids (except Acalypha indica, Camellia sinensis, Carotenoids (except Acalypha indica, Curcuma longa, and steroids (except Plectranthus amboinicus, Rauvolfia tetraphylla Quinones were found in one out of the five specimens. Some of the medicinal plant seemed to have potential as source of useful drugs. Though the one percent extracts of all the plants showed some degree of antimicrobial activity, it was significant in Acalypha indica, Camellia sinensis, Plectranthus amboinicus, Curcuma longa, and Rauvolfia tetraphylla. The extract of Camellia sinensis and Acalypha indica was most effective against Enterobacter faecalis (ZI = 3 cm and ZI = 1.7cm and Camellia sinensis and Acalypha indica was most effective against Staphylococcus aureus (ZI = 2.1 cm.

  9. Music, Synergies and Interculturality: "MUSSI at School" Project

    Science.gov (United States)

    Corte-Real, Maria De Sao Jose

    2008-01-01

    MUSSI is the name of a she-cat from an imaginary story invented and told by children from two elementary schools through different performing modalities. It is also the name of a pilot project of Applied Ethnomusicology funded by a major Portuguese Company, SONAE Sierra, through the local Loures Shopping Centre, which paid for the musical…

  10. INDRA - Regards sur le coeur de l'atome

    CERN Multimedia

    GANIL (CEA-CNRS) and Culture Production; Eusébio Serrano

    1993-01-01

    Réalisé début 1993 à l'occasion de l'inauguration du détecteur INDRA au GANIL (Grand Accélérateur National d'Ions Lours), ce film propose à travers le récit de la construction de cet instrument, une introduction à la physique nucléaire.

  11. Three more Mangrove trees growing locally in nature in freshwater

    NARCIS (Netherlands)

    Steenis, van C.G.G.J.

    1984-01-01

    In Christmas Island (Indian Ocean) the mangrove trees Bruguiera gymnorrhiza (L.) Sav., B. sexangula (Lour.) Poir. (Rhizophoraceae) and Heritiera littoralis Ait. (Sterculiaceae) are found inland near freshwater springs at some 20-30 m altitude, far from the beach. It is concluded that the inland stan

  12. [A herbalogical study on traditional Mongolian medicine "lideri"].

    Science.gov (United States)

    Bao, Y; Zhao, B; Bu, R

    2001-11-01

    By herbalogical study and investigation, "lideri" used by Mongolia doctors in different areas mainly contains 10 species from 4 genera of 4 families, but the quality materials only contains 3 species, Tinospora sinensis (Lour.) Merr., T. cordifolia Miers and T. capillipes Gagnep. PMID:15575169

  13. Systematic leaf anatomy of Baccaurea, Distichirhops, and Nothobaccaurea (Euphorbiaceae)

    NARCIS (Netherlands)

    Bodegom, S.; Haegens, R.M.A.P.; Heuven, van B.J.; Baas, P.

    2001-01-01

    The leaf anatomical diversity of the genera Baccaurea Lour. (43 species), Distichirhops Haegens (3 species) and Nothobaccaurea Haegens (2 species) (Euphorbiaceae) is described. Two species of Aporosa and three species of Maesobotrya were examined for comparison. The following characters are importan

  14. Isolation and characterization of new ceramides from aerial parts of Lepidaploa cotoneaster.

    Science.gov (United States)

    dos Santos, Edlene O; Meira, Marilena; do Vale, Ademir E; David, Jorge M; de Queiróz, Luciano P; David, Juceni P

    2012-06-01

    Two new ceramides were isolated from the bulbs of Lepidaploa cotoneaster (Willd. ex Spreng.) H. Rob. [Vernonia cotoneaster (Willd. ex Spreng.) Less.)], in addition to germanicol, beta-sitosterol, stigmasterol, 3-beta-O-beta-D-glucopyranosyl-sitosterol, lupeol, lupeoyl acetate and tiliroside. The structures of the new compounds were elucidated by spectral techniques (MS, 1H NMR, 13C NMR, HSQC, HMBC, DEPT, and TOCSY) and were compared with data reported in literature, and were established as 2S*,2'R*,3S*,4R*,11E)-N-[2'-hydroxyhenicosanoyl]-2-amino-nonadec-11-ene-1,3,4-triol (1) and (2S*,2'R*,3S*,4R*,8E)-N-[2'-hydroxytricosanoyl]-2-amino-nonadec-8-ene-1,3,4-triol (2). To establish the structure and to locate the double bond, the methyl ester of the fatty acid and dimethyl disulfide (DMDS) derivatives were prepared for both ceramides.

  15. Detection of Antimicrobial Compounds by Bioautography of Different Extracts of Leaves of Selected South African Tree Species

    OpenAIRE

    Suleimana, MM; McGaw, LJ; Naidoo, V.; Eloff, JN

    2009-01-01

    The hexane, acetone, dichloromethane and methanol extracts of Combretum vendae A.E. van Wyk (Combretaceae), Commiphora harveyi (Engl.) Engl. (Burseraceae), Khaya anthotheca (Welm.) C.DC (Meliaceae), Kirkia wilmsii Engl. (Kirkiaceae), Loxostylis alata A. Spreng. ex Rchb. (Anacardiaceae), Ochna natalitia (Meisn.) Walp. (Ochnaceae) and Protorhus longifolia (Bernh. Ex C. Krauss) Engl. (Anacardiaceae) were screened for their antimicrobial activity. The test organisms included bacteria (Enterococcu...

  16. Cytotoxic effect of Argentine medicinal plant extracts on human hepatocellular carcinoma cell line.

    Science.gov (United States)

    Ruffa, M J; Ferraro, G; Wagner, M L; Calcagno, M L; Campos, R H; Cavallaro, L

    2002-03-01

    Methanolic extracts from Achyrocline satureioides (Dc.) Lam, Aristolochia macroura Gomez, Lithraea molleoides (Vell.) Engl., Schinus molle L., unlike those from Celtis spinosa Spreng, Chenopodium ambrosioides L., Petiveria alliacea L., and Plantago major L. showed cytotoxic activity against a human hepatocellular carcinoma cell line, Hep G2. Schinus molle L. was the most active (IC50=50+/-7 microg/ml). These results call for further studies of these extracts.

  17. Cytotoxic effect of Argentine medicinal plant extracts on human hepatocellular carcinoma cell line.

    Science.gov (United States)

    Ruffa, M J; Ferraro, G; Wagner, M L; Calcagno, M L; Campos, R H; Cavallaro, L

    2002-03-01

    Methanolic extracts from Achyrocline satureioides (Dc.) Lam, Aristolochia macroura Gomez, Lithraea molleoides (Vell.) Engl., Schinus molle L., unlike those from Celtis spinosa Spreng, Chenopodium ambrosioides L., Petiveria alliacea L., and Plantago major L. showed cytotoxic activity against a human hepatocellular carcinoma cell line, Hep G2. Schinus molle L. was the most active (IC50=50+/-7 microg/ml). These results call for further studies of these extracts. PMID:11849838

  18. Structure of staminate flowers, microsporogenesis, and microgametogenesis in Helosis cayennensis var. cayennensis (Balanophoraceae)

    OpenAIRE

    González, Ana María; Popoff, Orlando Fabián; Salgado Laurenti, Cristina

    2013-01-01

    We analyzed the microgametogenesis and microsporogenesis of the male flowers of the holoparasitic Helosis cayennensis (Sw.) Spreng. var. cayennensis using optical and scanning electron microscopy. The unisexual flowers are embedded in a dense mass of uniseriate trichomes (filariae). Male flowers have a tubular 3-lobed perianth, with bilayered and non vascularized tepals. The androecium consists of three stamens with filaments and thecae connated into a synandrium. It has adnate a free central...

  19. Novedades agrostológicas (poaceae) para colombia, méxico y venezuela

    OpenAIRE

    GIRALDO-CAÑAS, DIEGO

    2012-01-01

    Se registran por primera vez para Colombia Aristida schiedeana Trin. & Rupr.,Calamagrostis guamanensis Escalona, Distichlis spicata (L.) Greene, Elionurusmuticus (Spreng.) Kuntze y Tripogon spicatus (Nees) Ekman. Por su parte, Axonopuscapillaris (Lam.) Chase es citada por primera vez para México, mientras queCalamagrostis jamesonii Steud. y Elionurus muticus se registran por primera vezpara Venezuela. Asimismo, se reporta por primera vez para la región andina a Raddiellaesenbeckii (Steud....

  20. Slope variation and population structure of tree species from different ecological groups in South Brazil

    OpenAIRE

    Edmilson Bianchini; Garcia, Cristina C.; José A. Pimenta; José M.D. Torezan

    2010-01-01

    Size structure and spatial arrangement of 13 abundant tree species were determined in a riparian forest fragment inParaná State, South Brazil (23"16'S and 51"01'W). The studied species were Aspidosperma polyneuron Müll. Arg., Astronium graveolens Jacq. and Gallesia integrifolia (Spreng) Harms (emergent species); Alseis floribunda Schott, Ruprechtia laxiflora Meisn. and Bougainvillea spectabilis Willd. (shade-intolerant canopy species); Machaerium paraguariense Hassl, Myroxylum peruiferum L. a...

  1. New species of Hydrellia (Diptera: Ephydridae) from the southern United States

    OpenAIRE

    Deonier, D. L.

    2010-01-01

    Five new species of Hydrellia Robineau-Desvoidy are described from localities in Alabama, Florida, Louisiana, and Texas. H. alabamae, n. sp. was first collected on water primrose in northern Alabama and later in northern Florida on Nuphar and Nymphoides. At present, there is no indication about potential host plant species. H. naiadis, n. sp. was reared from Najas guadalupensis (Spreng) Morong in northern Florida and later collected throughout the state and also in southeastern Texas. H. apal...

  2. Altimetry, Sea Surface Temperature and Ocean Colour Unveil the Effects of Planetary Waves on Phytoplankton

    Science.gov (United States)

    Cipollini, P.

    2006-07-01

    In the present paper w e discuss the manifestation of planetary w aves in sea surface temper atur e (SST) and ocean co lour, and the insigh t that can be gain ed by comparing those observations w ith altimetry. We focus in particular on the discovery of planetary waves in ocean co lour, which implies some eff ects of the wav es on phytoplankton. A critical assessment of the v arious mech anisms th at may be r esponsib le for th e formation of a signal in ocean colour h ighligh ts the importan t role played by horizon tal adv ection of phytoplankton. However, v ertical mechanisms su ch as upw elling of nutrien ts cannot b e ruled out comp letely at this stage, and there remains ample scope for a systematic global study of th e w ave signature in h eigh t, colour and SST.

  3. A New Furost-20(22)-ene Oligoglycoside from Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    A new furost-20(22)-ene oligoglycoside named as aspacochioside C was isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Its structure was elucidated to be 26-O-β- D-glucopyranosyl-(25S)-5β-furost-20(22)-en-3α,26-diol-3-O-α-L-rhamnopyranosyl-(1→4)-β-D- glucopyranoside on the basis of spectroscopic techniques including 1D and 2D NMR experiments.

  4. 秦岭种子植物区系新记录%Some Newly Recorded Spermatophytes to Qinling Mountain

    Institute of Scientific and Technical Information of China (English)

    黎斌; 李思锋; 袁永明; 张莹

    2013-01-01

    报道了秦岭种子植物区系4新记录属[杜若属(Pollia Thunb.)、美冠兰属(Eulophia R.Br.ex Lindl.)、常山属(Dichroa Lour.)和吊灯花属(Ceropegia Linn.)],8新记录种[川杜若Pollia miranda (Lévl.)Hara、长距美冠兰Eulophia dabia (D.Don) Hochr.、常山Dichroa febri fuga Lour.、肉色土(栾)儿 Apios carnea (Wall.)Benth.ex Baker、柳叶蓬莱葛Gardneria lanceolata Rehd.et Wils.、显脉獐牙菜Swertia nervosa (G.Don) Wall.ex C,B.Clarke、皱果赤瓟Thladiantha henryi Hemsl.var.henryi和宝兴吊灯花Ceropegia paohsingensis Tsiang et P.T.Li].%Four genera and eight species are reported as new records of spermatophyte flora to Qinling Mountain.4 newly recorded genera are Pollia Thunb.,Eulophia R.Br.ex Lindl.,Dichroa Lour.and Ceropegia Linn..8 newly recorded species are Pollia miranda (Lévl.) Hara (Commelinaceae),Eulophia dabia (D.Don) Hochr.(Orchidaceae),Dichroa febrifuga Lour.(Saxifragaceae),Apios carnea (Wall.)Benth.ex Baker (Leguminosae),Gardneria lanceolata Rehd.et Wils.(Loganiaceae),Swertia nervosa (G.Don) Wall.ex C.B.Clarke (Gentianaceae),Thladiantha henryi Hemsl.var.henryi (Cucurbitaceae) and Ceropegia paohsingensis Tsiang et P.T.Li (Asclepiadaceae).

  5. Antimicrobial and Cytotoxic Activity of Three Bitter Plants Enhydra Fluctuans, Andrographis Peniculata and Clerodendrum Viscosum.

    OpenAIRE

    Ruhul Amin, M.; Ripon Mondol; M. Rowshanul Habib; M. Tofazzal Hossain

    2012-01-01

    Purpose: In this study, three important medicinal plants (Enhydra fluctuans Lour, Clerodendrum viscosum Vent and Andrographis peniculata Wall) of Bangladesh were investigated to analyze their antimicrobial and cytotoxic activities against some pathogenic microorganisms and Artemia salina (brine shrimp nauplii). Methods: The coarse powder material of leaves of each plant was extracted separately with methanol and acetone to yield methanol extracts of leaves of Enhydra fluctuans (MLE), Cleroden...

  6. HPLC estimation of berberine in Tinospora cordifolia and Tinospora sinensis

    OpenAIRE

    Srinivasan G; Unnikrishnan K; Rema Shree A; Balachandran Indira

    2008-01-01

    A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80°). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of a...

  7. A new cytotoxic 2-(2-phenylethyl)chromone from Chinese eaglewood

    Institute of Scientific and Technical Information of China (English)

    Jun Liu; Jiao Wu; You Xing Zhao; Yuan Yuan Deng; Wen Li Mei; Hao Fu Dai

    2008-01-01

    A new compound 8-chloro-5,6,7-trihydroxy-2-(3-hydroxy-4-methoxyphenethyl)-5,6,7,8-tetrahydro-4H-chromen-4-one (1) was isolated from the Chinese eaglewood [Aquilaria sinensis (Lour.) Gilg]. Its structure was elucidated on the basis of spectral data. Compound 1 showed cytotoxicity against human gastric cancer cell line (SGC-7901) in vitro by MTT method with the IC50 value of 14.6 μg/mL.

  8. Gynura procumbens: An overview of the biological activities

    OpenAIRE

    Hui Li eTan; Kok Gan eChan; Priyia ePusparajah; Learn-Han eLee; Bey Hing eGoh

    2016-01-01

    Gynura procumbens (Lour.) Merr. (Family Asteraceae) is a medicinal plant commonly found in tropical Asia countries such as China, Thailand, Indonesia, Malaysia and Vietnam. Traditionally, it is widely used in many different countries for the treatment of a wide variety of health ailments such as kidney discomfort, rheumatism, diabetes mellitus, constipation and hypertension. Based on the traditional uses of G. procumbens, it seems to possess high therapeutic potential for treatment of variou...

  9. Gynura procumbens: An Overview of the Biological Activities

    OpenAIRE

    Tan, Hui-Li; Chan, Kok-Gan; Pusparajah, Priyia; Lee, Learn-Han; Goh, Bey-Hing

    2016-01-01

    Gynura procumbens (Lour.) Merr. (Family Asteraceae) is a medicinal plant commonly found in tropical Asia countries such as China, Thailand, Indonesia, Malaysia, and Vietnam. Traditionally, it is widely used in many different countries for the treatment of a wide variety of health ailments such as kidney discomfort, rheumatism, diabetes mellitus, constipation, and hypertension. Based on the traditional uses of G. procumbens, it seems to possess high therapeutic potential for treatment of vario...

  10. A Herbalogical Study on Traditional Mongolian Medicine "Lideri"%蒙药利得日的本草考证

    Institute of Scientific and Technical Information of China (English)

    宝音图; 赵百岁; 布日额

    2001-01-01

    经调查考证,今蒙藏各地传统医学所用的利得日各有不同,其原植物有4科4属10种,其中正品利得日的原植物应为宽筋藤Tinospora sinensis(Lour.)Merr、心叶宽筋藤Tinospora cordifolia Miers和金果榄Tinospora capillipes Gagnep.等三种藤本植物.

  11. 山苍子野生苗造林和挥发油土法蒸馏技术%Reforestation of Litsea cubeba (Lout.)Pers.with wild seedling and indigenous method of distilling out its volatile oil

    Institute of Scientific and Technical Information of China (English)

    刘良源

    1988-01-01

    @@ 山苍子[Litsea cubeba(Lour)Pers]俗称野胡椒、山鸡椒、木姜子.属樟科.是我国南方主要芳香油料树种.其挥发油可提取紫罗蓝酮,是食品、化妆品的主要香料,也是我国出口的重要物资之一.

  12. Plants used in the treatment of leishmanial ulcers due to Leishmania (Viannia braziliensis in an endemic area of Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Flávio França

    1996-06-01

    Full Text Available This paper records the plants used in the treatment of cutaneous leishmaniasis due to Leishmania (Viannia braziliensis (L(Vb among the rural population of a cocoa- producing coastal area of Bahia state, Brazil. An enquiry conducted among a hundred patients identified 49 plant species used to treat skin ulceration caused by this Leishmania species. The principal plants used are caju-branco (Anacardium occidentale - Anacardiaceae, used by 65% of the population, folha-fogo (Clidemia hirta - Melastomataceae 39%, alfavaca-grossa (Plectranthus amboinicus - Lamiaceae 33%, mastruz (Chenopodium ambrosioides - Chenopodiaceae 31%, erva-de-santa-maria (Solatium americanum - Solanaceae (25% and transagem (Plantago major - Plantaginaceae. 2%.

  13. Atividade antimicrobiana in vitro de produtos vegetais em otite externa aguda In vitro antimicrobial activity of plants in Acute Otitis Externa

    OpenAIRE

    Janaina Cândida Rodrigues Nogueira; Margareth de Fátima Melo Diniz; EDELTRUDES O. LIMA

    2008-01-01

    Otite externa aguda é a inflamação do conduto auditivo externo, e plantas medicinais podem ser utilizadas, na cultura popular, para seu tratamento. OBJETIVO: Avaliar atividade antimicrobiana in vitro de Aleolanthus suaveolens, Caryophyllus aromaticus, Cymbopogon citratus, Matricaria chamomila, Pithecellobium avaremotemo, Plectranthus amboinicus e Ruta graveolens sobre agentes etiológicos de otite externa. CASUÍSTICA E MÉTODOS: A concentração inibitória mínima de extratos e óleos destas planta...

  14. Composição do óleo essencial de quatro espécies do gênero Plectranthus Essential oil composition of four Plectranthus species

    OpenAIRE

    J.M Bandeira; F.F. Barbosa; L.M.P Barbosa; I.C.S. Rodrigues; M.A Bacarin; Peters, J A; E.J.B Braga

    2011-01-01

    O gênero Plectranthus é considerado um dos mais ricos em óleos essenciais dentro da família Lamiaceae, compreendendo muitas espécies com propriedades medicinais. Algumas destas são conhecidas popularmente como boldo, as quais possuem semelhanças taxonômicas e diversas sinonímias, possuindo ações anti-dispépticas, analgésicas e digestivas. O objetivo deste estudo foi avaliar quantitativa e qualitativamente os óleos essenciais presentes nas folhas das espécies P. amboinicus, P. barbatus, P. gra...

  15. Plants used in the treatment of leishmanial ulcers due to Leishmania (Viannia) braziliensis in an endemic area of Bahia, Brazil.

    Science.gov (United States)

    França, F; Lago, E L; Marsden, P D

    1996-01-01

    This paper records the plants used in the treatment of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis (L(V)b) among the rural population of a cocoa-producing coastal area of Bahia state, Brazil. An enquiry conducted among a hundred patients identified 49 plants species used to treat skin ulceration caused by this Leishmania species. The principal plants used are caju-branco (Anacardium occidentale, Anacardiaceae), used by 65% of the population, folha-fogo (Clidemia hirta,Melastomataceae) 39%, alfavaca-grossa (Plectranthus amboinicus, Lamiaceae) 33%, mastruz (Chenopodium ambrosioides, Chenopodiaceae) 31%, erva-de-santa-maria (Solanum americanum, Solanaceae) (25%) and transagem (Plantago major, Plantaginaceae) 2%. PMID:8701041

  16. The genus Plectranthus in India and its chemistry.

    Science.gov (United States)

    Waldia, Shobha; Joshi, Bipin C; Pathak, Uma; Joshi, Mukesh C

    2011-02-01

    Phytochemical constituents isolated from Indian species of the genus Plectranthus reported up to 2009 are compiled. In India, the genus Plectranthus is found in all the habitats and altitudes, particularly in the Himalaya, the Southern Ghats, and the Nilgiri region. P. amboinicus, P. barbatus, P. caninus, P. mollis, P. coetsa, and P. incanus are the most common species found in India. Phytochemical studies of the genus revealed that Indian Plectranthus species are rich in essential oil, and that the most abundant secondary metabolites are diterpenoids, i.e., labdanes, abietanes, and ent-kauranes, as well as triterpenoids. PMID:21337498

  17. Genetic similarities among four species of the Plectranthus (L’Hér. genus - DOI: 10.4025/actascibiolsci.v32i1.880 Genetic similarity among four species of Plectranthus genus - DOI: 10.4025/actascibiolsci.v32i1.880

    Directory of Open Access Journals (Sweden)

    Eugenia Jacira Bolacel Braga

    2009-12-01

    Full Text Available The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus and the intraspecific diversity of P. barbatus collected from different places in southern Brazil, by means of the RAPD technique. A higher genetic similarity was observed between the P. neochilus and P. amboinicus species (80%, followed by P. grandis and P. barbatus (77%. P. barbatus genotypes from Passo Fundo and Porto Alegre showed a genetic similarity which was close to 100%, while the genetic similarity for P. barbatus genotypes from other locations was higher than 96%. Although a low variability among genotypes of this species was found in this study, RAPD markers allowed a clear differentiation among the analyzed genotypes, showing a 53% mean genetic similarity, with a high correlation value (r = 0.99, which proves a high agreement between the genetic similarity and clustering data.Medicinal plants are widely used by the population, therefore they are important sources of natural chemical products. Plectranthus includes species related as “boldo” that have anti-dyspeptics, analgesic and stimulants of digestion properties. Related species or genotypes can differ widely in the amount of natural active principles, in such case the lack of genetic fitness can lead to the use of not appropriate plants to the population health. Molecular techniques can be used for DNA-fingerprinting of plants providing its correct identification. This work aimed at verifying the genetic diversity among four species of Plectranthus genus (P. grandis, P. barbatus, P

  18. Plants used in the treatment of leishmanial ulcers due to Leishmania (Viannia braziliensis in an endemic area of Bahia, Brazil

    Directory of Open Access Journals (Sweden)

    Flávio França

    1996-06-01

    Full Text Available This paper records the plants used in the treatment of cutaneous leishmaniasis due to Leishmania (Viannia braziliensis (L(Vb among the rural population of a cocoa- producing coastal area of Bahia state, Brazil. An enquiry conducted among a hundred patients identified 49 plant species used to treat skin ulceration caused by this Leishmania species. The principal plants used are caju-branco (Anacardium occidentale - Anacardiaceae, used by 65% of the population, folha-fogo (Clidemia hirta - Melastomataceae 39%, alfavaca-grossa (Plectranthus amboinicus - Lamiaceae 33%, mastruz (Chenopodium ambrosioides - Chenopodiaceae 31%, erva-de-santa-maria (Solatium americanum - Solanaceae (25% and transagem (Plantago major - Plantaginaceae. 2%.Este trabalho relata as plantas usadas no tratamento da leishmaniose cutânea, causada por Leishmania (Viannia braziliensis (L(Vb, na população rural da faixa litorânea produtora de cacau do estado da Bahia, Brasil. Um inquérito realizado entre 100 pacientes, identificou 49 espécies de plantas usadas para tratar úlceras de pele causadas por esta espécie de Leishmânia. As principais plantas usadas foram o cajueiro-branco (Anacardium occidentale - Anacardiaceae usado por 65% da população, a folha-fogo (Clidemia hirta - Melastomataceae 39%, a alfavaca-grossa (Plectranthus amboinicus - Lamiaceae 33%, o mastruz (Chenopodium ambrosioides - henopodiaceae 31%, a erva-de-santa-maria (Solanum americanum - Solanaceae 25% e a transagem (Plantago major - Plantaginaceae 2%.

  19. Apoptosis Effect of Girinimbine Isolated from Murraya koenigii on Lung Cancer Cells In Vitro

    Directory of Open Access Journals (Sweden)

    Syam Mohan

    2013-01-01

    Full Text Available Murraya koenigii Spreng has been traditionally claimed as a remedy for cancer. The current study investigated the anticancer effects of girinimbine, a carbazole alkaloid isolated from Murraya koenigii Spreng, on A549 lung cancer cells in relation to apoptotic mechanistic pathway. Girinimbine was isolated from Murraya koenigii Spreng. The antiproliferative activity was assayed using MTT and the apoptosis detection was done by annexin V and lysosomal stability assays. Multiparameter cytotoxicity assays were performed to investigate the change in mitochondrial membrane potential and cytochrome c translocation. ROS, caspase, and human apoptosis proteome profiler assays were done to investigate the apoptotic mechanism of cell death. The MTT assay revealed that the girinimbine induces cell death with an IC50 of 19.01 μM. A significant induction of early phase of apoptosis was shown by annexin V and lysosomal stability assays. After 24 h treatment with 19.01 μM of girinimbine, decrease in the nuclear area and increase in mitochondrial membrane potential and plasma membrane permeability were readily visible. Moreover the translocation of cytochrome c also was observed. Girinimbine mediates its antiproliferative and apoptotic effects through up- and downregulation of apoptotic and antiapoptotic proteins. There was a significant involvement of both intrinsic and extrinsic pathways. Moreover, the upregulation of p53 as well as the cell proliferation repressor proteins, p27 and p21, and the significant role of insulin/IGF-1 signaling were also identified. Moreover the caspases 3 and 8 were found to be significantly activated. Our results taken together indicated that girinimbine may be a potential agent for anticancer drug development.

  20. Taxonomic novelties in Mikania (Asteraceae: Eupatorieae) from Atlantic Forest, Brazil

    OpenAIRE

    Borges, R.A.X.; Forzza, R.C.; Fraga, C.N.

    2010-01-01

    During studies of Brazilian Atlantic Forest Asteraceae, a new species and a replacement name were determined: Mikania amorimii from Bahia State and Mikania capixaba from Espírito Santo State. The former is a new species related to M. ternata but distinct by its leaves, involucral bracts and cypsela morphology. The latter is proposed as a replacement name for Mikania dentata G.M.Barroso, a later homonym of M. dentata Spreng. Line drawings and comments about conservation status are made for bot...

  1. Review on studies of Eupatorium adenophorum--an important invasive species in China

    Institute of Scientific and Technical Information of China (English)

    SUNXiao-yu; LUZhao-hua; SANGWei-guo

    2004-01-01

    Eupatorium adenophorum Spreng. was introduced in Yunnan Province of China around 1940. Since then it has been spreading rapidly, particularly in the southern and southwestern parts of China and caused serious economic loss. The biological research and integrated control on E. adenophorum were carried out from 1980's in Yunnan Province. Together with other 15 invasive external species, the weed has been listed in the White Paper by The State Environmental Protection Administration of China. This paper briefly reviews thestudies on natural distribution, biological character, ecological character, chemical component, hazard, potential application and the control of E. adenophorum. The research direction for this invasive external species in future was also discussed.

  2. Fungi Associated with Capororoca (Myrsine ferruginea) Myrsinaceae Fruits and Seeds Fungos Associados aos Frutos e Sementes de Capororoca (Myrsine ferruginea) Myrsinaceae

    OpenAIRE

    Suelen Santos Rego; Álvaro Figueredo dos Santos; Antonio Carlos de Souza Medeiros

    2010-01-01

    The objectives of this study were to identify the fungi associated with capororoca (Myrsine ferruginea Spreng) fruits and seeds from different lots and check the incidence of fungi between green and ripe fruits and
    seeds from these fruits. For fungi detection Blotter test (BT) method was utilized, being 400 fruits and 400 seeds each sample, and incubated at 20 ºC ±1 ºC, 12 h dark light/12 h complete dark, for seven days, followed by the evaluation of the fungi incidence (%). It was...

  3. Medicamentos herbarios en el centro-oeste argentino, V. Clematis montevidensis var. montevidensis, caracterización de la droga

    OpenAIRE

    Petenatti, Marta E.; Álvarez, María E; Petenatti, Elisa M.; Del Vitto, Luis A.; Saad, José R.; Mauricio R. Téves; Giordano, Oscar S.

    2005-01-01

    Clematis montevidensis Spreng. var. montevidensis (Ranunculaceae) es una liana denominada vulgarmente "barba de viejo", "cabello de ángel", "bejuco" o "loconte", empleada en medicina popular por sus propiedades rubefacientes hasta vesicantes en la región centro-oeste de la Argentina, donde está ampliamente distribuida. Ensayos recientes han demostrado que la droga ejerce fuerte acción irritante y moderada acción diurética. Para definir adecuadamente sus caracteres y contribuir al efectivo con...

  4. О НОВЫХ И НАИБОЛЕЕ РЕДКИХ ВИДАХ ТАМБОВСКОЙФЛОРЫ. СООБЩЕНИЕ 4

    OpenAIRE

    Соколов, Александр; Соколова, Людмила

    2014-01-01

    В работе сообщаются сведения о новых (Astragalus pubiflorus (Pall.)) DC., Veronica persica Poir., Dipsacus strigosus Willd. ex Roem. et Schult.) и наиболее редких (Ephedra distachya L., Stipa lessingiana Trin. et Rupr., S. pulcherrima C. Koch, Rhynchospora alba (L.) Vahl, Carex humilis Leysser, Veratrum nigrum L., Anthericum ramosum L., Lilium martagon L., Ceratocarpus arenarius L., Arenaria procera Spreng., Silene sibirica (L.) Pers., Actaea spicata L., Aconitum anthora L., Clematis integrif...

  5. Sesquiterpene lactones. XXXVI. Sesquiterpene lactones in several subgenera of the genus Centaurea L.

    Directory of Open Access Journals (Sweden)

    Gerard Nowak

    2014-02-01

    Full Text Available The occurrence of both known and new sesquiterpene lactones was determined in six species classified in different subgenera of Centaurea L. Chlorojanerin, cynaropicrin and janerin were isolated from C. phaeopappoides Bordz. and C. thracica (Janka Hayek. C. marschalliana Spreng. was found to contain acroptilin, chlorojanerin, cebellin D and janerin while C. adjarica Alb. had repin, acroptilin, chlorojanerin, centaurepensin, janerin, repidiolide, cebellin D, E, F and L A new, hitherto underscribed cebellin J quaianolide was found in C. bella Trautv. and another germacranolide, 3α, 15-dihydroxycostunolide was found in C. sphaerocephala subsp. lusitanica (Boiss. et Reuter Nyman.

  6. USE OF ULTRASOUND FOR ESTIMATIVE OF MECHANICAL PROPERTIES OF Peltophorum dubium WOOD

    OpenAIRE

    Diego Martins Stangerlin; Darci Alberto Gatto; Rafael Rodolfo de Melo; Leandro Calegari; Magnos Alan Vivian; Patrícia Aparecida Rigatto Castelo; Rafael Beltrame

    2010-01-01

    The aimed this study was evaluate the use of dynamic elastic constant obtained by non-destructive test as a parameter in the estimative of mechanical properties of Peltophorum dubium (Spreng.) Taub. wood obtained by destructive test. For this, ultrasonic equipment (not-destructive method) was utilized with point-contact transducersof 45 kHz. The ultrasonic velocity was determined considering the wave transmission along the length of the samples, which dimensions of 2.5 x 2.5 x 41 cm in thickn...

  7. Two New Furostanol Glycosides from Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Two new furostanol oligoglycosides named as aspacochioside A (1) and B (2) were isolated from the roots of Asparagus cochinchinensis (Lour.) Merr.. Their structures were elucidated to be 3-O-[{α-L-rhamnopyranosyl-(1→4)}{β-D-glucopyranosyl}]-26-O-[β-D-glucopy- ranosyl]-(25S)-5β-furostane-3β,22α,26-triol 1 and 3-O-[{α-L-rhamnopyranosyl-(1→4)}{(-D-glu- copyranosyl}]-26-O-[β-D-glucopyranosyl]-22α-methoxy-(25S)-5β-furostane-3β,26-diol 2 on the basis of spectroscopic techniques and chemical methods.

  8. Cochinchin from Dracaena cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    HE,Lan(何兰); WANG,Zhu-Hong(王竹红); LIU,Xue-Hui(刘雪辉); FANG,De-Cai(方德彩); Li,Hua-Min(李华民)

    2004-01-01

    A new compound, Cochinchin (1), together with 7,4'-dihydroxyflavone (2), 7-hydroxy-4'-methoxyflavane (3),7-hydroxy-3-(4-hydroxybenzyl)chroman (4), 4'-hydroxy-2,4-dimethoxydihydrochalcone (5) and 4'-hydroxy-2,4,6-trimethoxydihydrochalcone (6) was isolated from the resin (trivial name, "dragon's blood") of Dracaena cochinchinensis (Lour.) S. C. Chen. The structure of 1 was elucidated on the basis of spectroscopic data as (2,3-trans)-6-ally1-2-(3,5-dimethoxyphenyl)-3-(4-hydroxyphenyl)-2,3-dihydrobenzo[1,4]dioxin which is a natural product possessing a new framework.

  9. Antropologia Funerária e Paleobiologia das Populações Pós-Medievais Portuguesas: Os Casos de Nossa Senhora da Anunciada e Espírito Santo

    OpenAIRE

    Ferreira, Nathalie Antunes

    2015-01-01

    As desigualdades sociais na saúde constituem a problemática abordada nesta dissertação, tendo sido escolhidas para explorar esta questão duas séries esqueléticas – igreja de Nossa Senhora da Anunciada (Setúbal) e capela do Espírito Santo (Loures) – cronologicamente enquadradas no período pós-medieval, entre os séculos XVI e XIX, e cujos indivíduos apresentavam estatutos socioeconómicos diferenciados. Na concretização deste objectivo foi realizada uma primeira abordagem que incidiu no estud...

  10. 防治变态反应的功能食品

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    该功能食品的组成为:枸桔Poncirus trifoliata(L.)Rafin.30%-80%(质量分数,下同)、三白草Saururus chinensis(Lour.)Baill.5%-20%、五味子Schisandra chinensis(Turcz.)Baill.5%-25%、车前Plantago asiatica L.提取物5%-30%。收集、清洗和干燥的上述草药,分别置于50-60倍水中,

  11. HPLC Estimation of berberine in Tinospora cordifolia and Tinospora sinensis

    OpenAIRE

    Srinivasan, G. V.; Unnikrishnan, K.P.; A B Rema Shree; Balachandran, Indira

    2008-01-01

    A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80°). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:wat...

  12. 几种麦冬及其伪品的组织学鉴别比较研究%Comparative Study on Histological Identification of Tuber of Dwarf Lilyturf and False Medical Material

    Institute of Scientific and Technical Information of China (English)

    刘义梅

    2005-01-01

    对麦冬的药典品种麦冬Ophiopogon japonicus(Thunb)Ker-Gawl,地方品种湖北麦冬Liriope spicata(Thunb.)Lour.var.proliferaY.T.Ma及阔叶麦冬Liriope platyphylla Wang et Tang,伪品淡竹根Lophatherum gracile Brongn.及石刁柏根Asparagus officinalis L.进行了组织学鉴别、显微摄影和特征比较等方面的研究.

  13. Anisotropy magnetic susceptibility measurements of vulcanic rock from merapi mountain in central Java

    International Nuclear Information System (INIS)

    Anisotropy Magnetic susceptibility indicated a differences of Magnetic susceptibility value of a sample due to the direction or orientation of magnetic field on it. The 22 sample's were taken from lour area around Merapi mountain in central Java and their Anisotropy Magnetic susceptibility were measured by using MS2 Bartington. The 22 sample's shown a high susceptibility value about 8037.5 x 105. Eleven sample's have high anisotropy ( it's anisotropy degree about 16% ). The rest of the sample have an anisotropy degree less than 6% (sample's from pasar bubar, Kali Kuning, Kali Gendong, Kali Gendol Utara). This result give an indication that a part of the sample's can be used for paleomagnetic

  14. Responses in root physiological characteristics of Vallisneria natans (Hydrocharitaceae) to increasing nutrient loadings

    OpenAIRE

    Cai X; Yao L; Gao G.; Xie Y; Yang J; Tang X; Zhang M

    2016-01-01

    We selected the submerged macrophyte Vallisneria natans (Lour.) Hara for investigating the effects of nutrient loadings (nitrogen (N)-phosphorus (P) in mg·L-1: (1) 0.5, 0.05; (2) 1.0, 0.1; (3) 5.0, 0.5; (4) 10.0, 1.0) on root physiological characteristics using sand culture during the growth season (June to October). Results showed that the best root growth was in macrophytes exposed to moderate nutrient conditions (N-P 2, in ...

  15. 自制莲黄苏打液治疗隐翅虫皮炎394例

    Institute of Scientific and Technical Information of China (English)

    刘彦; 梁瑜; 曾桥; 李彬彬; 程爱兰; 郭勤

    2002-01-01

    @@ 1999年9月10月,我院新建学生宿舍区出现大量毒隐翅虫(Paederus),从而引起隐翅虫皮炎(Paederus dermatitis)的爆发流行.在学校整治宿舍周边环境的同时,我教研室利用半边莲(Lobelia cknensis Lour)、藤黄(Garcinia henaburyiHook)等配制的莲黄苏打液用于治疗学生的隐翅虫皮炎,取得了较好的疗效,特报道如下:

  16. 中国南方叶刺瘿螨亚科3新种记述(蜱螨亚纲,瘿螨总科)%Three New Species of Phyllocoptinae (Acari, Eriophyoidea) from South China

    Institute of Scientific and Technical Information of China (English)

    朱辉; 韦绥概; 李国军

    2009-01-01

    randiae sp. nov. infesting Randia sinensis (Lour.) Schultes (Rubiaceae) are described and illustrated. All mites are vagrant on the undersurfaces of host leaves.%本文记述了叶刺瘿螨亚科Phyllocoptinae 3新种:菝葜离子瘿螨Leipothrkr smilax sp.nov.,白饭树顶冠瘿螨Tegohrphus virosae sp.nov.,鸡爪簕顶冠瘿螨Tegohrphus randiae sp.nov..本文所用量度单位均为微米(μm).模式标本保存在广西大学农学院.

  17. New Distribution of Paederia in Rubiaceae in t he Northeast of China%东北茜草科的一个新分布属和种

    Institute of Scientific and Technical Information of China (English)

    王青; 李艳; 陈辰

    2001-01-01

    The plant of Paederia in Rubiaceae has never been found in the northeast of China.Chinese Fevervine Paederia scan dens is new distribution in the northeast of China indeed.This is first report ed.%茜草科鸡矢藤 属(Paederia)的植物以前在东北地区未曾发现,经过鉴定,鸡矢藤Paederia(Lour. )Merr.确属茜草科在东北地区的1个新分布属种,此次首次报道.

  18. Nocturnal sleep disturbance through aircraft noise. Do wakening reactions begin at maximum levels of 60 decibels (A)?; Naechtliches Erwachen durch Fluglaerm. Beginnen Aufwachreaktionen bei Maximalpegeln von 60 Dezibel(A)?

    Energy Technology Data Exchange (ETDEWEB)

    Maschke, C. [Technische Univ. Berlin (Germany). Inst. fuer Technische Akustik; Hecht, K. [Inst. fuer psychosoziale Gesundheit, Berlin (Germany); Wolf, U. [Bundesgesundheitsamt, Berlin (Germany). Robert-Koch-Institut

    2001-10-01

    The present paper is not concerned with the question whether it makes sense from the viewpoint of preventive medicine to use the onset of noise-induced wakening reactions as the sole criterion for nocturnal noise protection. On this point reference is made to the mediation process ''Extension of Frankfurt am Main Airport''. The attending experts (Bullinger, Griefhahn, Hecht, Kastka, Maschke, Spreng) were agreed that 52 to 53 dB (A) should be regarded as the maximum nocturnal noise level at which vegetative impairment begins. According to present knowledge the minimum protection goal that must be demanded, therefore, is to ensure that sleeping persons are exposed to maximum continuous nocturnal noise levels of less than 52 dB (A). [German] Im vorliegenden Beitrag wird nicht der Frage nachgegangen, ob es praeventivmedizinisch sinnvoll ist, ein naechtliches Schutzkriterium allein auf den Beginn von laermbedingten Aufwachreaktionen abzustellen. In diesem Zusammenhang ist auf das Mediationsverfahren ''Ausbau Flughafen Frankfurt/Main'' hinzuweisen. Von den anwesenden Experten (Bullinger, Griefahn, Hecht, Kastka, Maschke, Spreng) wurde uebereinstimmend ein naechtlicher Maximalpegel von 52 bis 53 dB(A) als Beginn fuer vegetative Beeintraechtigungen angesehen. Nach heutigen Kenntnisstand ist daher als minimales Schutzziel zu fordern, dass regelmaessige naechtliche Maximalpegel von 52 dB(A) am Ohr des Schlaefers vermieden bzw. unterschritten werden sollten. (orig.)

  19. Novelties in Casearia (Flacourtiaceae for Argentina NOVEDADES EN CASEARIA (FLACOURTIACEAE PARA ARGENTINA

    Directory of Open Access Journals (Sweden)

    Héctor A. Keller

    2010-07-01

    Full Text Available

    Casearia obliqua Spreng. (Flacourtiaceae is recorded for the first time to Argentina, based on
    specimens collected beside Iguazú river, in Misiones province. The taxon is here described
    and illustrated. The presence of Casearia gossypiosperma Briq. in Misiones, a tree mentioned
    in 1936 for that province, is here documented by mean herbarium material. A key for identifying
    the argentinean species is included
    Se cita por primera vez para la flora de Argentina, Casearia obliqua Spreng. (Flacourtiaceae,
    sobre la base de ejemplares recolectados junto al río Iguazú, en la provincia de Misiones. Este
    taxón es descripto e ilustrado. Se certifica mediante material de herbario la presencia en
    Misiones de Casearia gossypiosperma Briq., especie arbórea que fue mencionada para dicha
    provincia en 1936. Se incluye una clave para determinar las especies de Casearia de
    Argentina.

  20. Comparative leaf morphology and anatomy of three Asteraceae species

    Directory of Open Access Journals (Sweden)

    Patricia Milan

    2006-01-01

    Full Text Available The objective of this paper was to describe and compare the morphology and anatomy of mature leaves of Mikania glomerata Spreng., Porophyllum ruderale Cass. and Vernonia condensata Baker (Asteraceae species that have different habits emphasizing their secretory structures. Longitudinal and transversal sections of mature leaf blades of the three species were analyzed at the apex, base, and medium third part of the midvein of the leaf blade and of the margin. M. glomerata had uniseriate glandular trichomes and secretory ducts; P. ruderale had hydathodes and secretory cavities; and V. condensata had idioblasts and uni-and biseriate glandular trichomes.Este trabalho teve por objetivo descrever e comparar a morfo-anatomia das folhas adultas de Mikania glomerata Spreng., Porophyllum ruderale Cass. e Vernonia condensata Baker (Asteraceae, que possuem diferentes hábitos, enfatizando suas estruturas secretoras. Secções longitudinais e transversais dos limbos foliares foram analisadas nas regiões do ápice, da base e do terço médio na altura da nervura central, do limbo foliar e da margem. M. glomerata apresentou tricomas glandulares unisseriados e ductos secretores; P. ruderale tinha hidatódios e cavidades secretoras; e V. condensata apresentou idioblastos e tricomas glandulares uni- ou bisseriados.

  1. Harm,Comprehensive Control and Utilization of Invasion Plant E.adenophorum%入侵植物紫茎泽兰的危害及综合防控与利用

    Institute of Scientific and Technical Information of China (English)

    周启武; 于龙凤; 王绍梅; 李晓君; 彭泽琴; 赵宝玉

    2014-01-01

    入侵植物紫茎泽兰对我国生态环境、生物多样性、农林渔牧业发展以及人类健康等社会经济造成严重危害。论文在对文献资料分析、归纳的基础上,就入侵植物紫茎泽兰的生物学性状、地理分布、灾害情况、综合防控技术及开发利用研究现状进行综述,探讨了今后的研究方向,为充分研究和开发利用紫茎泽兰提供参考。%The invasive plant E.adenophorum Spreng have caused the serious threat to the socio-economic of ecological environment,biodiversity,development of agriculture,forestry,fishery and animal husband-ry,and human health in China.According to the analysis and summary of data reported,research status of biological characteristics,geographical distribution,harmful situation,comprehensive control techniques, exploitation and utilization of E.adenophorum Spreng were reviewed and discussed in this paper,in order to provide the important theoretical basis to research,exploit and utilize E.adenophorum.

  2. Plant diversity of sacred groves and its comparative account with surrounding denuded hills from Bhor region of Western Ghats

    Directory of Open Access Journals (Sweden)

    Hangarge L. M.

    2016-07-01

    Full Text Available Sacred groves in western Maharashtra are patches of forest preserved on religious ground. Only these preserved forest patches remain undamaged whereas the surrounding areas have scanty or eroded vegetation. Many studies show that the floristic surveys of sacred groves were carried out without emphasizing the comparative account with surrounding vegetation. In the present investigation we selected four sacred groves i.e. Somjaichi Rai, Maulidevichi Rai, Nivgunjaichi Rai, and Umberjaichi Rai; and focussed on comparative study between the floristic composition of sacred groves and its surrounding areas. These sacred groves look like a single isolated patch of forest; whereas the surrounding area supports very scanty trees and shrubs. The selected sacred groves depict such situation. Plant diversity in each sacred grove was very unique than its surrounding open areas. Some common species in all sacred groves are Acacia concinna DC, Bridelia retusa (L. Spreng, Cassia fistula L., Mangifera indica L., Erythrina varigeta L., Meyna laxiflora Robyns, Syzygium cumini (L. Skeels and Terminalia chebula Retz. Some rarely occurring plants were Entada rheedei Spreng, Zingiber cerneum Dalz and Zingiber neesanum (Grah. Raman. We compared between these sacred groves and its surrounding areas at taxa level.

  3. Slope variation and population structure of tree species from different ecological groups in South Brazil.

    Science.gov (United States)

    Bianchini, Edmilson; Garcia, Cristina C; Pimenta, José A; Torezan, José M D

    2010-09-01

    Size structure and spatial arrangement of 13 abundant tree species were determined in a riparian forest fragment in Paraná State, South Brazil (23°16'S and 51°01'W). The studied species were Aspidosperma polyneuron Müll. Arg., Astronium graveolens Jacq. and Gallesia integrifolia (Spreng) Harms (emergent species); Alseis floribunda Schott, Ruprechtia laxiflora Meisn. and Bougainvillea spectabilis Willd. (shade-intolerant canopy species); Machaerium paraguariense Hassl, Myroxylum peruiferum L. and Chrysophyllum gonocarpum (Mart. & Eichler ex Miq.) Engl. (shade-tolerant canopy species); Sorocea bonplandii (Baill.) Bürger, Trichilia casaretti C. Dc, Trichilia catigua A. Juss. and Actinostemon concolor (Spreng.) Müll. Arg. (understory small trees species). Height and diameter structures and basal area of species were analyzed. Spatial patterns and slope correlation were analyzed by Moran's / spatial autocorrelation coefficient and partial Mantel test, respectively. The emergent and small understory species showed the highest and the lowest variations in height, diameter and basal area. Size distribution differed among emergent species and also among canopy shade-intolerant species. The spatial pattern ranged among species in all groups, except in understory small tree species. The slope was correlated with spatial pattern for A. polyneuron, A. graveolens, A. floribunda, R. laxiflora, M. peruiferum and T. casaretti. The results indicated that most species occurred in specific places, suggesting that niche differentiation can be an important factor in structuring the tree community. PMID:21562693

  4. Atividade antimicrobiana in vitro de produtos vegetais em otite externa aguda In vitro antimicrobial activity of plants in Acute Otitis Externa

    Directory of Open Access Journals (Sweden)

    Janaina Cândida Rodrigues Nogueira

    2008-02-01

    Full Text Available Otite externa aguda é a inflamação do conduto auditivo externo, e plantas medicinais podem ser utilizadas, na cultura popular, para seu tratamento. OBJETIVO: Avaliar atividade antimicrobiana in vitro de Aleolanthus suaveolens, Caryophyllus aromaticus, Cymbopogon citratus, Matricaria chamomila, Pithecellobium avaremotemo, Plectranthus amboinicus e Ruta graveolens sobre agentes etiológicos de otite externa. CASUÍSTICA E MÉTODOS: A concentração inibitória mínima de extratos e óleos destas plantas foi obtida em amostras de otite externa. RESULTADOS: Staphylococcus aureus em 10 culturas, Pseudomonas aeruginosa em 8, Pseudomonas aeruginosa e Staphylococcus aureus, em associação, em 5 culturas e Candida albicans e Candida krusei em 4 culturas. P. aeruginosa foi resistente a todos os extratos e óleos essenciais testados; os extratos de A. suaveolens, P. avaremotemo e de R. graveolens foram inativos, o óleo essencial de C. aromaticus e M. chamomila foram ativos contra 3 cepas de S. aureus e as cepas de Candida; Sete das cepas de S. aureus foram sensíveis ao extrato de P. amboinicus, mas o óleo não mostrou atividade, 4 cepas de S.aureus e as cepas de Candida foram sensíveis ao óleo essencial de R. graveolens. CONCLUSÃO: Algumas plantas apresentaram resultados satisfatórios, dependendo do agente etiológico, porém se faz necessário estudos mais detalhados, para melhorar o aproveitamento destas plantas.Acute Otitis Externa is an inflammation of the outer auditory meatus, and according to popular saying, medicinal plant extracts can be used in its treatment. AIM: to assess the in vitro antimicrobial activity of the following plants: Aleolanthus suaveolens; Caryophyllus aromaticus; Cymbopogon citratus; Matricaria chamomila; Pithecellobium avaremotemo; Plectranthus amboinicus and Ruta graveolens on the germs that cause otitis externa. MATERIALS AND METHODS: the minimum inhibitory concentration of extracts and oils from these plants was

  5. Protective effect of carvacrol on acute lung injury induced by lipopolysaccharide in mice.

    Science.gov (United States)

    Feng, Xiaosheng; Jia, Aiqing

    2014-08-01

    Carvacrol, the major component of Plectranthus amboinicus, has been known to exhibit anti-inflammatory activities. The aim of this study was to investigate the effects of carvacrol on lipopolysaccharide (LPS)-induced endotoxemia and acute lung injury (ALI) in mice. Mice were injected intraperitoneally (i.p.) with LPS and the mortality of mice for 7 days were observed twice a day. Meanwhile, the protective effect of carvacrol (20, 40 or 80 mg/kg) on LPS-induced endotoxemia were detected. Using an experimental model of LPS-induced ALI, we examined the effect of carvacrol in resolving lung injury. The results showed that carvacrol could improve survival during lethal endotoxemia and attenuate LPS-induced ALI in mice. The anti-inflammatory mechanisms of carvacrol may be due to its ability to inhibit NF-κB and MAPKs signaling pathways, thereby inhibiting inflammatory cytokines TNF-α, IL-6 and IL-1β production. PMID:24577726

  6. Composição do óleo essencial de quatro espécies do gênero Plectranthus Essential oil composition of four Plectranthus species

    Directory of Open Access Journals (Sweden)

    J.M Bandeira

    2011-01-01

    Full Text Available O gênero Plectranthus é considerado um dos mais ricos em óleos essenciais dentro da família Lamiaceae, compreendendo muitas espécies com propriedades medicinais. Algumas destas são conhecidas popularmente como boldo, as quais possuem semelhanças taxonômicas e diversas sinonímias, possuindo ações anti-dispépticas, analgésicas e digestivas. O objetivo deste estudo foi avaliar quantitativa e qualitativamente os óleos essenciais presentes nas folhas das espécies P. amboinicus, P. barbatus, P. grandis e P. neochilus. A extração do óleo foi realizada por hidrodestilação, utilizando pentano como solvente extrator, repetida por três vezes para cada uma das espécies. A análise dos componentes dos óleos essenciais das quatro espécies de Plectranthus, através da CG/EM, permitiu identificar 14 componentes químicos, sendo a maioria sesquiterpenos. O trans-cariofileno se apresentou em elevada concentração nos óleos estudados. Alguns componentes químicos demonstraram ser específicos para cada espécie e outros apresentaram ocorrência comum a todas as quatro, possibilitando a diferenciação das mesmas em dois grupos, um formado por P. amboinicus e P. neochilus e o outro por P. grandis e P. barbatus. Conclui-se que as quatro espécies de boldo apresentam diferenças significativas quanto ao teor e à constituição química do óleo essencialPlectranthus has been considered one of the richest genera in essential oils within the Lamiaceae family, which includes several species with medicinal properties. Some of them are commonly known as boldo and present taxonomic similarities and several synonymies, with antidyspeptic, analgesic and digestive actions. The aim of this study was to evaluate quantitatively and qualitatively the essential oils from P. amboinicus, P. barbatus, P. grandis and P. neochilus leaves. The oil was extracted by hydrodistillation using pentane as extracting solvent and was repeated three times for each

  7. Genetic similarities among four species of the Plectranthus (L’Hér. genus = Similaridade genética de quatro espécies do gênero Plectranthus

    Directory of Open Access Journals (Sweden)

    Juliana de Magalhães Bandeira

    2010-01-01

    Full Text Available The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus and the intraspecific diversity of P. barbatus collected from different places in southern Brazil, by means of the RAPD technique. A higher genetic similarity was observed between the P. neochilus and P. amboinicus species (80%, followed by P. grandis and P. barbatus (77%. P. barbatus genotypes from Passo Fundo and Porto Alegre showed a genetic similarity which was close to 100%, while the genetic similarity for P. barbatus genotypes from other locations was higher than 96%. Although a low variability among genotypes of this species was found in this study, RAPD markers allowed a clear differentiation among the analyzed genotypes, showing a 53% mean genetic similarity, with a high correlation value (r = 0.99, which proves a high agreement between the genetic similarity and clustering data.O gênero Plectranthus abrange plantas de diversas espécies, referidas como boldo, que são utilizadas na medicina popular pelas suas propriedades antidispépticas, analgésicas e estimulantes da digestão. Genótipos relacionados podem diferir amplamente na quantidade de princípios ativos naturais, resultando no uso de plantas não-apropriadas à saúde da população. O objetivo do presente estudo foi avaliar a diversidade genética interespecífica de quatro espécies do gênero Plectranthus (P. grandis, P. barbatus, P. neochilus e P. amboinicus e intraespecífica de P. barbatus, coletadas em diferentes localidades da região Sul do Brasil, por meio da técnica de RAPD

  8. Early Evaluation of Compatibility between Commercial Citrus Varieties and Kaffir Lime (Citrus hystrix and Carrizo Citrange (C. sinensis Osb. x P. trifoliata L. Raf. Rootstocks at Mekong Delta, Vietnam

    Directory of Open Access Journals (Sweden)

    Le Thi Khoe

    2015-01-01

    Full Text Available The results of investigation on early evaluation of compatibility between commercial citrus varieties, and Kaffir lime (Citrus hystrix and Carrizo citrange (C. sinensis Osb. x P. trifoliata L. Raf. rootstocks, at Mekong Delta Vietnam, during four successive years from 2010 to 2013, reported that out of the tested combinations of the recent commercial citrus varieties, included Da xanh pumelo (C. grandis Osbeck, seedless Mat orange (C. sinensis L. Osbeck, Sanh orange (C. nobilis Lour. and Duong mandarin  (C. reticulata, budded on Kaffir lime and Carrizo citrange, in the 3rd and 4th  year after planting under the trial fields at Mekong Delta Vietnam, the trees of Sanh orange (C. nobilis Lour. budded on Kaffir lime (Citrus hystrix were supperior with precocity, producing high number of fruits (16.67 fruits per tree , and very good fruit quality of larger fruit size (334.6 g and 11.16 cm, fruit weight and diameter, respectively, higher total soluble solids (12.5 brix, better orange-yellow juice color development, and excellent flavor and aroma of juice, as compared to the typical characteristics; meanwhile these were followed by trees of seedless Mat orange budded on Kaffir lime, which produced the highest number of fruits (47.33 fruits per tree, with little inferior quality of seedy fruit (1-3 seeds per fruit, lower total soluble solids (7.33%, and no good flavor and aroma, as somewhat differed from the typical fruit traits.

  9. 中药天冬研究进展%Research Progress of Chinese Medicine Asparagus cochinchinensis

    Institute of Scientific and Technical Information of China (English)

    韦树根; 马小军; 柯芳; 黄宝优; 付金娥

    2011-01-01

    Asparagus cochinchinensis (Lour.) Merr was a kind of perennial liana plant from Asparagus Linn, of liliaceae and was one of the traditional Chinese medicine. The study on resource distribution, chemical composition, pharmacological activi-ty and clinical applications of A. Cochinchinensis in last decade was summarized. It provided a reference for the further de-velopment of A. Cochinchinensis.%天冬[Asparagus cochinchinensis (Lour.)Merr]为百合科天门冬属藤本植物,是中国传统的常用大宗中药材之一.文章分析了近十几年来国内外对天冬的研究概况,对其资源分布、化学成分、药理活性及临床应用等方面的进展进行了阐述,为天冬的进一步开发利用研究提供参考依据.

  10. STRUKTUR DAN KOMPOSISI JENIS HUTAN MANGROVE DI GOLO SEPANG – KECAMATAN BOLENG KABUPATEN MANGGARAI BARAT

    Directory of Open Access Journals (Sweden)

    M. Hidayatullah

    2014-09-01

    Full Text Available This study aimed to determine mangrove forest structure and composition in Golo Sepang Village, Manggarai Barat District. Important value index and diversity index value obtained from mangrove vegetation analysis were used as indicators for determining mangrove forest structure and diversity. Transect method with square frame along the line was applied in vegetation analysis. Totally 10 lines and 30 plots were applied for getting types of mangrove, growth parametersvalue (height and diameter and others related information. This study found that the stucture of mangrove consist of 5 familyes with 10 species, namely: Rhizophoraceae (Ceriops tagal (Perr, Rhizophora apiculata (Bi, R. mucronata Lmk., Bruguiera parviflora (Roxb., B. sexangula (Lour dan B. gymnorrizha (L. Lamk., Fabaceae (Derris trifoliata Lour, Meliaceae (Xylocarpus granatum Koen, Pteridaceae (Acrosthicum aereum Linn and Lythraceae (Phempis acidula Forst. R. apiculata (Bi is the most dominant species founded in 7 of 10 total sites. Two sites, Sotri and Muara Kiri, have the highest important value index (300% for R. Apiculata species. The highest individual density is found at Sotri site, with value 1.300 tree/hectare, while the lowest density, 100 trees/hectare is found at Muara Kanan site. In diversity of mangrove, all sites were categorized as low with highest diversity index value 1,06.Keywords: Mangrove forest, structure and composition, Golo Sepang

  11. The leaf essential oils of five Vietnamese Desmos species (Annonaceae).

    Science.gov (United States)

    Dai, Do Ngoc; Hoi, Tran Minh; Thang, Tran Dinh; Ogunwande, Isiaka A

    2012-02-01

    The leaf essential oils of five Desmos species from Vietnam have been extracted by steam distillation and subjected to GC and GC-MS analyses. The plant samples were Desmos cochinchinensis Lour., D. penduculosus (A. DC.) Ban, D. penducolosus var. tonkinensis Ban, D. chinensis Lour., and D. dumosus (Roxb.) Saff. The oils were rich in sesquiterpene hydrocarbons (65.9%-88.9%) and monoterpene hydrocarbons (6.3%-30.9%). The oxygenated counterparts were less common. The quantitatively significant constituents of the oils were alpha-pinene (2.4%-12.1%), beta-elemene (2.2-39.5%), beta-caryophyllene (13.9-26.3%), germacrene D (9.9-15.5%), bicyclogermacrene (2.0-11.4%) and alpha-humulene (3.8-7.5%). The studied oils could be classified into two chemical forms: oils with abundance of beta-caryophyllene, germacrene D and alpha-pinene (D. cochinchinensis, D. penducolosus var. tonkinensis, D. chinensis and D. Dumosus) and oil with high amounts of beta-elemene, beta-caryophyllene and germacrene D (D. penduculosus).

  12. 宽筋藤的本草考证

    Institute of Scientific and Technical Information of China (English)

    吴凤荣; 曾聪彦; 戴卫波; 梅全喜; 陈小露

    2015-01-01

    通过文献检索,查阅、整理有关宽筋藤的本草记载资料,并进行对比分析和归纳总结.宽筋藤之名始见于《生草药性备要》,其后多部岭南药学本草都有记载,但其基原品种尚不确定,有防己科中华青牛胆Tinospora sinensis(Lour.) Merr.或石松科石松Lycopodium japonicum Thunb.ex Murray、铺地蜈蚣Lycopodium cerumum L等多种来源的可能.直至1959年出版的《广西中兽医药用植物》才首次以防己科植物中华青牛胆Tinospora sinensis (Lour.) Merr.正品明确收载.传统中医药以其藤茎部分人药,主要有舒筋活络、祛风止痛的功效;民族医药则以其藤茎、叶、根入药,除有舒筋活络、祛风止痛的功效外,还有补虚、调经等作用.

  13. Ethnomedicinal plants of Jodhpur District, Rajasthan used in herbal and folk remedies

    Directory of Open Access Journals (Sweden)

    B.B.S.Kapoor

    2013-12-01

    Full Text Available The Jodhpur district a part of Thar Desert is very rich in medicinal plant wealth. The medicinal plants of this region have great potential to be used in drug and pharmaceutical industries. These herbal plants have been used by local people, tribal communities, vendors, native doctors such as Ojhas, Bhagats Bhopas and experts of Ayurvedic fields since long time in herbal and folk remedies. Kalbelia, Nats, Bhils, Raika, Bhopas, Banjara, Gadolia-Lohar, Saharia and Meena communities of this district have a rich knowledge of plants based traditional medicines. Ten ethnomedicinal plants like Cleome gynandra Linn., Clerodendrum phlomidis Linn., Cassia angustifolia Vahl., Echinops echinatus Roxb., Leucas aspera (Willd. Spreng., Mimosa hamata Willd., Moringa oleifera Lamk., Pedalium murex Linn., Peganum harmala Linn., Sida cordifolia Linn. have been selected for this research work. The present investigation is aimed to create awareness about the ethnomedicinal value of the plants and their uses to draw the attention of pharmacologists, phytochemists and pharmaceuticals.

  14. Four newly recorded species of Dryopteridaceae from Kashmir valley, India

    Directory of Open Access Journals (Sweden)

    SHAKOOR AHMAD MIR

    2014-04-01

    Full Text Available Mir SA, Mishra AK, Reshi ZA, Sharma MP. 2014. Four newly recorded species of Dryopteridaceae from Kashmir valley, India. Biodiversitas 15: 6-11. Habitat diversity, elevation, cloud cover, rainfall, seasonal and temperature variations have created many ideal sites for the luxuriant growth of pteridophytes in the Kashmir valley, yet all the regions of the valley have not been surveyed. In Kashmir valley the family Dryopteridaceae is represented by 31 species. During the recent extensive field surveys of Shopian district four more species viz., Dryopteris caroli-hopei Fraser-Jenkins, Dryopteris blanfordii subsp. nigrosquamosa (Ching Fraser-Jenkins, Dryopteris pulvinulifera (Bedd. Kuntze and Polystichum Nepalense (Spreng C. Chr. have been recorded for the first time from the valley. The taxonomic description, synonyms, distribution and photographs of each species are given in this article.

  15. ANTIFUNGAL AND CYTOTOXIC ACTIVITIES OF FIVE TRADITIONALLY USED INDIAN MEDICINAL PLANTS

    Directory of Open Access Journals (Sweden)

    Adhikarimayum Haripyaree

    2013-02-01

    Full Text Available Hexane, Methanol and Distilled water extracts of five Indian Medicinal plants viz., Mimosa pudica L, Vitex trifolia Linn, Leucas aspera Spreng, Centella asiatica (L Urban and Plantago major Linn belonging to different families were subjected to preliminary antimicrobial screening against six standard organisms viz., Ceratocystis paradoxa, Aspergillus niger, Penicillium citrinum, Macrophomina phaseoli, Trichoderma viride and Rhizopus nigricans. To evaluate antifungal activity agar well diffusion method was used. In addition LD50 of the same plant extracts were determined by using Range test on Mus musculus for cytotoxic activity. Methanolic extract of M. pudica showed the highest and significant inhibitory effect against some fungal species. Again, methanolic extract of M. pudica displayed the greatest cytotoxic activity.

  16. 广东省野生树种地理分布新记录%The New Geographical Distribution Records of Wild Tree Species in Guangdong Province, China

    Institute of Scientific and Technical Information of China (English)

    韩维栋; 陈杰

    2013-01-01

    对广东省雷州半岛各地野生木本植物实地踏查与标本采集鉴定,并参照相关文献,发现广东省地理分布新记录树种6个,分别是:硬骨藤(Pycnarrhena poilanei(Gagnep.)Forman)、光叶海桐(Pittosporum glabratum Lindl.)、台琼海桐(Pittosporum pentandrum (blanco) Merr.var.hainanense(Gagnep.)Li.)、铁线子(Manilkara hexandra(Roxb.)Dubard)、光叶柿(Diospyros diversilimba Merr.et Chun)、玉蕊(Barringtonia racemosa(Linn.)Spreng.).

  17. Disturbance is required for CO2-dependent promotion of woody plant growth in grasslands

    DEFF Research Database (Denmark)

    Loveys, Beth R.; Egerton, John J. G.; Bruhn, Dan;

    2010-01-01

    The relative effects of disturbance (here defined as bare soil), competition for edaphic resources, thermal interference and elevated [CO2] on growth of tree seedlings in grasslands were studied under field conditions. Snow gum (Eucalyptus pauciflora Sieb. ex Spreng.) seedlings were grown in open...... resources or competitive enhancement of environmental stress. Nevertheless, elevated [CO2] could promote invasion of grasslands due to enhancement of woody plant growth in bare soil created by disturbances.......-top chambers flushed with either ambient or elevated [CO2] from March 2004 to January 2005 (autumn to summer). These seedlings were planted into three treatments (i.e. bare soil, soil covered with straw or soil supporting a sward of live pasture grass) to separate effects of grass on seedling growth into those...

  18. Citogenética de espécies arbóreas da subfamília Caesalpinioideae-Leguminosae do sul do Brasil

    Directory of Open Access Journals (Sweden)

    Elaine Biondo

    2005-01-01

    Full Text Available Chromosome counts and meiotic behaviour analysis are excellent contributions for studies on taxonomic relationships and evolutionary patterns in plants. The tree legume species of the subfamily Caesalpinioideae have been poorly analysed cytogenetically. This work aimed at determining the number of chromosomes and analysing the meiotic behaviour in 11 species of Caesalpinioideae. Basic chromosome numbers in most of the analysed species were 2n = 28 (x = 14. Senna multijuga (L. C. Rich. H. S. Irwin & Barneby and Schizolobium parahyba (Vell. Blake presented 2n = 24 (x = 12 and Peltophorum dubium (Spreng. Taub. 2n = 26 (x = 13. In all the species nuclei presented an areticulate pattern and a regular meiosis in the six species studied. Further collections and cytogenetic analyses with a major number of individuals and species are suggested, in order to provide additional data for a more comprehensive study on this group of plants.

  19. Slope variation and population structure of tree species from different ecological groups in South Brazil

    Directory of Open Access Journals (Sweden)

    Edmilson Bianchini

    2010-09-01

    Full Text Available Size structure and spatial arrangement of 13 abundant tree species were determined in a riparian forest fragment inParaná State, South Brazil (23"16'S and 51"01'W. The studied species were Aspidosperma polyneuron Müll. Arg., Astronium graveolens Jacq. and Gallesia integrifolia (Spreng Harms (emergent species; Alseis floribunda Schott, Ruprechtia laxiflora Meisn. and Bougainvillea spectabilis Willd. (shade-intolerant canopy species; Machaerium paraguariense Hassl, Myroxylum peruiferum L. and Chrysophyllum gonocarpum (Mart. & Eichler ex Miq. Engl. (shade-tolerant canopy species; Sorocea bonplandii (Baill. Bürger, Trichilia casaretti C. Dc, Trichilia catigua A. Juss. and Actinostemon concolor (Spreng. Müll. Arg. (understory small trees species. Height and diameter structures and basal area of species were analyzed. Spatial patterns and slope correlation were analyzed by Moran's / spatial autocorrelation coefficient and partial Mantel test, respectively. The emergent and small understory species showed the highest and the lowest variations in height, diameter and basal area. Size distribution differed among emergent species and also among canopy shade-intolerant species. The spatial pattern ranged among species in all groups, except in understory small tree species. The slope was correlated with spatial pattern for A. polyneuron, A. graveolens, A. floribunda, R. laxiflora, M. peruiferum and T. casaretti. The results indicated that most species occurredin specific places, suggesting that niche differentiation can be an important factor in structuring the tree community.Visando contribuir para o conhecimento das estratégias devida de espécies em fragmentos florestais, foram determinadas as estruturas de tamanho e espacial de 13 espécies arbóreas do remanescente de floresta ciliar no Estado do Paraná, no Sul do Brasil (23"16'S e 51"01'W. Foram analisadas as espécies: Aspidosperma polyneuron Müll. Arg., Astronium graveolens Jacq. e Gallesia

  20. Estimation of electric power generation using coal from the fruit peel of cupuassu in Amazonas State, Brazil; Estimativa de geracao de energia eletrica utilizando o carvao da casca do fruto do cupuacuzeiro no estado do Amazonas

    Energy Technology Data Exchange (ETDEWEB)

    Silva, Antonio Cleuder Lima da [Manaus Energia S/A., Manaus, AM (Brazil); Santos, Eyde Cristiane Saraiva dos [Universidade Federal do Amazonas (FCA/UFAM), Manaus, AM (Brazil). Faculdade de Ciencias Agrarias. Dept. de Engenharia Agricola e Solos], e-mail: eyde_cristianne@yahoo.com.br

    2008-07-01

    The application of the charcoal of agroforestry residue for the generation of electric energy, for being renewable power plant and possessing technology for application, comes being evidenced. In this research the potential of generation of electric energy was estimated, in the main producing cities of the State of Amazonas of the fruit of the cupuacuzeiro (Theobroma grandiflorum Wild. Ex. Spreng. Shum.), using the rind carbonized in technology of gasification, substitution of diesel. The charcoal of the rind of the fruit of the cupuacuzeiro presents to be able calorific next to the one to the deriving coal of the wood. Known the annual production of each one the cities, the amount and the cost of production of the generated electric energy, determined in this research, it justifies the exploitation of this residue, for having economic advantages, when comparative with the tariff of the adopted electric energy in the region for the concessionaire. (author)

  1. In vitro cytotoxic activity of Brazilian Middle West plant extracts

    Directory of Open Access Journals (Sweden)

    Talal Suleiman Mahmoud

    2011-06-01

    Full Text Available Cytotoxic activity of eight plant extracts, native from the Mid-West of Brazil comprising Cerrado, Pantanal and semideciduous forest, was evaluated for MDA-MB-435, SF-295, and HCT-8 cancer cell strains. A single 100 µg.mL-1 dose of each extract was employed with 72 h of incubation for all tests. Doxorubicin (1 µg.mL-1 was used as the positive control and the MTT method was used to detect the activity. Cytotoxicity of distinct polarities was observed in thirty extracts (46%, from different parts of the following species: Tabebuia heptaphylla (Vell. Toledo, Bignoniaceae, Tapirira guianensis Aubl., Anacardiaceae, Myracrodruon urundeuva Allemão, Anacardiaceae, Schinus terebinthifolius Raddi, Anacardiaceae, Gomphrena elegans Mart., Amaranthaceae, Attalea phalerata Mart. ex Spreng., Arecaceae, Eugenia uniflora L., Myrtaceae, and Annona dioica A. St.-Hil., Annonaceae. Extracts of at least two tested cell strains were considered to be highly active since their inhibition rate was over 75%.

  2. Alternative dentistry with medicinal plants in Chapada dos Guimarães – Mato Grosso – Brazil

    Directory of Open Access Journals (Sweden)

    Aneliza Meireles BORBA

    2008-04-01

    Full Text Available Introduction: In Mato Grosso, the use of plant species as a therapeutic alternative is passed through generations. Objective: This research aimed at a survey of the vegetal species used by the neighborhood of Santa Cruz community, Chapada dos Guimarães city, their therapeutical indications and methods of use to the oral health. Material and methods: Forty local informers were questioned through qualitative approach. The collection of 65 vegetable species was cataloged and filed for identification in the UFMT/Herbário Central. Results and conclusion: The most cited for teeth eruption was chamomile(Matricaria chamomilla L.; to stomatitis, the saffron (Crocus sativus L.; to tooth pain, the “arnica-da-serra” (Brickelia brasiliensis (Spreng.(Robinson. The leaf was the most used part of the plant, and the tea, by decoction, the most common method of use.

  3. NOVEDADES EN HOUSSAYANTHUS Y SERJANIA (SAPINDACEAE

    Directory of Open Access Journals (Sweden)

    María Silvia Ferrucci

    2010-11-01

    Full Text Available Sobre la base de Urvillea sparrei Barkl, el combiriation nueva Houssayanthus sparrei (Barkl. Ferrucci se establece, y su taxonomía es discutida en relación con las otras especies del género: H.macrolophus (Radlk. Hunzik. y H.fiebrigii (Barkl. Hunzik.Cromosoma. número de H.sparrei se informa (12 II. Un nuevo nombre, Serjania herteri Ferrucci se propone para S.australis (St. Hil. Herter no Spreng.

  4. Complete assignments of NMR data and assessment of trypanocidal activity of new eremantholide C derivatives

    Energy Technology Data Exchange (ETDEWEB)

    Saude-Guimaraes, Denia Antunes, E-mail: saude@ef.ufop.br, E-mail: saudeguima@gmail.com [Universidade Federal de Ouro Preto (UFOP), MG (Brazil); Raslan, Delio S.; Chiari, Egler; Oliveira, Alaide B. de [Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG (Brazil)

    2014-12-15

    Chemical transformations of eremantholide C (1), a sesquiterpene lactone that was isolated from Lychnophora trichocarpha Spreng. led to five new derivatives: 1’,2’- epoxyeremantholide C (2), 5-n-propylamine-4,5-dihydro-1’,2’-epoxyeremantholide C (3), 5-n-propylammonium-4,5-dihydro-1’,2’-epoxyeremantholide C chloride (4), 5-n-propylammonium-4,5-dihydroeremantolide C chloride (5) and 16-O-ethyleremantholide C (6). The structures of all these derivatives were assigned on the basis of IR, MS, {sup 1}H and {sup 13}C NMR data by 1D and 2D techniques. Eremantholide C and the derivatives 2, 4 and 5 were evaluated against trypomastigotes Y and CL strains of Trypanosoma cruzi. Eremantholide C completely inhibited the growth of both the parasites strains while all derivatives were partially active against the CL strain and inactive against the Y strain. (author)

  5. Growth analysis of Fafia in relation to the propagation method used Análise de crescimento de fáfia em função do tipo de propagação

    Directory of Open Access Journals (Sweden)

    André May

    2008-09-01

    Full Text Available A trial was carried out in Campinas, Brazil, from August 2005 to August 2006, in order to analyze the effect of two propagation methods (seeds and cuttings on the development of Pfaffia glomerata (Spreng. Pedersen. The experimental design was a completely randomized block, with five replications, and two methods of plant propagation. Plant height, aerial part dry mass and root dry mass were evaluated. Plants propagated from cuttings showed greater accumulation of dry mass on the aerial part. Root dry mass accumulation curves showed an exponential pattern, and at the end of the experimental period, the average of roots dry mass for both treatments were similar, up to 349,65 g plant-1.Neste trabalho, realizado no Instituto Agronômico, em Campinas-SP, de agosto/05 a agosto/06, estudou-se a influência de dois tipos de propagação no desenvolvimento de Pfaffia glomerata (Spreng. Pedersen. O delineamento experimental utilizado foi inteiramente casualizado com cinco repetições, sendo nos tratamentos, estudadas duas formas de propagação da planta (semente e estacas. Foi avaliada a altura da planta, massa seca da parte aérea e massa seca da raiz. As plantas propagadas por estaca apresentaram maior acúmulo de massa seca da parte aérea comparativamente àquelas propagadas por sementes. As curvas de acúmulo de massa seca da raiz apresentaram comportamento exponencial, sendo que, ao final do período experimental, a produção de raízes foi semelhante para os dois tratamentos estudados, atingindo valores de até 349,65 g planta-1 de massa seca da raiz.

  6. Reproductive biology of the Iberian species of Potentilla L. (Rosaceae

    Directory of Open Access Journals (Sweden)

    Guillén, Antonio

    2005-06-01

    Full Text Available Several processes related to the reproductive biology of the Iberian species of the genus Potentilla L. subg. Trichothalamus (Spreng. Rchb., Comarum (L. Syme and Fragariastrum (Heist. ex Fabr. Rchb. are studied. We provide data concerning compatibility, agamospermy and hybridization along with pollination and fruit dispersal. No agamospermy mechanisms were found in any of the taxa, nor were there signs of any hybridization, although potential signs of self-incompatibility were detected. Regarding to pollination, a marked tendency toward autogamy was observed in certain groups. Finally, some mechanisms for the release and dispersal of diaspores are described in relation to diaspore and flower receptacle morphology.Se exponen los resultados del estudio de algunos mecanismos de la biología reproductiva en las especies ibéricas de Potentilla L. subg. Trichothalamus (Spreng. Rchb., Comarum (L. Syme y Fragariastrum (Heist. ex Fabr. Rchb. Se aportan datos que afectan a la compatibilidad, agamospermia e hybridización a lo largo de las fases de polinización y dispersión del fruto. No se observaron mecanismos de agamospermia en ninguno de los táxones estudiados ni tampoco se comprobó hibridación alguna, aunque se detectaron signos potenciales de autoincompatibilidad. Relacionado con la polinización, se observó en ciertos grupos una clara tendencia a la autogamia. Se describen, por último, algunos mecanismos de liberación y dispersión de diásporas, que están relacionados con la morfología de la propia diáspora y del receptáculo floral.

  7. Estrutura fitossociológica da vegetação arbórea do Parque Natural Municipal de Santo Ângelo, Santo Ângelo, RS

    Directory of Open Access Journals (Sweden)

    Alexandre Hüller

    2011-12-01

    Full Text Available O presente trabalho foi realizado no Parque Natural Municipal de Santo Ângelo (RS, pertencente à Prefeitura Municipal de Santo Ângelo, com o objetivo de avaliar a composição florística e estrutura fitossociológica da floresta, em uma área de 13 hectares. Para tanto, foram utilizadas 12 parcelas de tamanho 10 x 20 m distribuídas sistematicamente na área, nas quais foram medidos e identificados todos os indivíduos com DAP maior ou igual a 10 cm. Os resultados obtidos foram analisados e processados com o uso do programa FITOANÁLISE, calculando-se os parâmetros fitossociológicos de densidade, dominância, frequência, valor de importância, valor de cobertura, índice de diversidade de Shannon H' e índice de Morisita. Foram encontradas 35 espécies, pertencentes a 30 gêneros e 21 famílias, das quais branquilho (Sebastiania commersoniana (Baill. L. B. Sm. & Downs, açoita-cavalo (Luhea divaricata Mart, maria-preta (Diatenopterix sorbifolia Radlk e canela-preta (Nectandra megapotamica Spreng. foram as que apresentaram maior representatividade nos parâmetros fitossociológicos do componente arbóreo desta floresta. As espécies com maior Valor de Importância (VI foram açoita-cavalo (Luhea divaricata Mart com 50,53; branquilho (Sebastiania commersoniana (Baill. com 46,24; maria-preta (Diatenopterix sorbifolia Radlk com 26,93; canela-preta (Nectandra megapotamica Spreng. com 20 e farinha-seca (Machaerium stipitatum Vogel com 16,64. Já na estrutura vertical da floresta, Luehea divaricata apresentou maiores valores no estrato superior e Sebastiania commersoniana Baill. nos estratos médio e inferior.

  8. UHPLC-PDA-ESI-TOF/MS metabolic profiling of Arctostaphylos pungens and Arctostaphylos uva-ursi. A comparative study of phenolic compounds from leaf methanolic extracts.

    Science.gov (United States)

    Panusa, Alessia; Petrucci, Rita; Marrosu, Giancarlo; Multari, Giuseppina; Gallo, Francesca Romana

    2015-07-01

    The aim of this study was to get a rapid metabolic fingerprinting and to gain insight into the metabolic profiling of Arctostaphylos pungens H. B. K., a plant morphologically similar to Arctostaphylos uva-ursi (L.) Spreng. (bearberry) but with a lower arbutin (Arb) content. According to the European Pharmacopoeia the Arb content in the dried leaf of A. uva-ursi (L.) Spreng. must be at least 7% (wt/wt) but other species, like A. pungens, are unintentionally or fraudulently marketed instead of it. Therefore, methanolic leaf extracts of nine A. uva-ursi and six A. pungens samples labeled and marketed as "bearberry leaf" have been analyzed. A five-minute gradient with a UHPLC-PDA-ESI-TOF/MS on an Acquity BEH C18 (50×2.1 mm i.d.) 1.7 μm analytical column has been used for the purpose. A comprehensive assignment of secondary metabolites has been carried out in a comparative study of the two species. Among twenty-nine standards of natural compounds analyzed, fourteen have been identified, while other fifty-five metabolites have been tentatively assigned. Moreover, differences in both metabolic fingerprinting and profiling have been evidenced by statistical multivariate analysis. Specifically, main variations have been observed in the relative content for Arb, as expected, and for some galloyl derivative like tetra- and pentagalloylglucose more abundant in A. uva-ursi than in A. pungens. Furthermore, differences in flavonols profile, especially in myricetin and quercetin glycosilated derivatives, were observed. Based on principal component analysis myricetrin, together with a galloyl arbutin isomer and a disaccharide are herein proposed as distinctive metabolites for A. pungens. PMID:25702282

  9. Transfer of uranium and radium to Chinese cabbage from soil containing elevated levels of natural radionuclides

    International Nuclear Information System (INIS)

    The transfer of 238U and 226Ra to Chinese cabbage (Brassica rapa L. subsp. pekinensis (Lour.) Hanelt) was investigated from soils contaminated with uranium-mill tailings (UMT) by means of a pot experiment in laboratory-based conditions applying different levels of soil contamination under various growing conditions. Activity concentrations for 226Ra in Chinese cabbage varied from 56-276, 156-502 and 277-877 Bq kg-1 dry mass for 20, 40 and 60 % of UMT content in the soil, respectively, and for 238U from 1.0-2.3 and 2.3-4.7 Bq kg-1 dry mass for 40 and 60 % of UMT content in the soil, respectively. The results showed increased accumulation of 226Ra and low accumulation of 238U in cabbage leaves in more contaminated soil. (author)

  10. 九种植物抽提物对小菜蛾产卵驱避作用的研究%Studies on repelling action of extracts from 9 species of plants against Plutella xylostella L.

    Institute of Scientific and Technical Information of China (English)

    彭跃峰; 庞雄飞

    2004-01-01

    以干扰作用控制指数(IIPC)为评价指标,测定了8个科9种植物茎叶乙醇抽提物对小菜蛾成虫的产卵驱避作用.结果表明,反枝苋(Amaranthus retroflexus L.)、茜草(Rubiaceae L.)、打碗花(Calystegia hedracea Wall.)、猪毛草(Scirpus wallichii Nees.)和粪箕笃(Stephania longa Lour.)对小菜蛾均有较好的驱避作用,处理24h后的驱避效果达80%以上;其对小菜蛾的驱避作用随施用后时间的延长而逐渐减弱.

  11. 非嗜食植物次生物质对小菜蛾实验种群的干扰作用%Interference Effect on the Experiment Popoulation of Plutella xylostella with By-substance from Its Disliking Plant

    Institute of Scientific and Technical Information of China (English)

    彭跃峰; 庞雄飞

    2004-01-01

    以干扰作用控制指数(IIPC)为评价指标,研究了20种非嗜食植物乙醇抽提物对小菜蛾实验种群的干扰作用.结果表明,白花蒿(Artemisla lactiflora Wall.)、反枝苋(Amaranthus retroflexus L.)、茜草(Rubia cordifolia L.)、飞机草(Chromolaena odorata L.)、打碗花(Calystegia hederalea Wall.)、粪箕笃(Sephania. longa Lour.)、刺儿菜(Cephalanoplos segetum Bge.)、猪毛草(Scirpus wallichii Nees.)对小菜蛾实验种群具有较强的干扰作用,干扰作用控制指数均在0.2以下.

  12. Interference effects of extracts of non-preferable plants on experimental population of Plutella xylostella%非嗜食植物次生化合物对小菜蛾实验种群的干扰作用

    Institute of Scientific and Technical Information of China (English)

    彭跃峰

    2005-01-01

    以干扰作用控制指数(IIPC)为评价指标,评价了20种非嗜食植物乙醇抽提物对小菜蛾实验种群的控制作用.结果表明,白花蒿Artemisia lactiflora Wall.、反枝苋Amaranthus retroflexus L.、茜草Rubia cordifolia L.、飞机草Chromolaena odorata L.、打碗花Calystegia hederacea Wall.、粪箕笃Stephania longga Lour.、刺儿菜Cephalanoplos segetum Bge.、猪毛草Scirpus wallichii Nees.对小菜蛾实验种群具有较强的干扰作用,干扰作用控制指数均在0.2以下.

  13. Dragon's blood dropping pills have protective effects on focal cerebral ischemia rats model.

    Science.gov (United States)

    Xin, Nian; Yang, Fang-Ju; Li, Yan; Li, Yu-Juan; Dai, Rong-Ji; Meng, Wei-Wei; Chen, Yan; Deng, Yu-Lin

    2013-12-15

    Dragon's blood is a bright red resin obtained from Dracaena cochinchinensis (Lour.) S.C.Chen (Yunnan, China). As a traditional Chinese medicinal herb, it has great traditional medicinal value and is used for wound healing and to stop bleeding. Its main biological activity comes from phenolic compounds. In this study, phenolic compounds were made into dropping pills and their protective effects were examined by establishing focal cerebral ischemia rats model used method of Middle Cerebral Artery Occlusion (MCAO), and by investigating indexes of neurological scores, infarct volume, cerebral index, cerebral water content and oxidation stress. Compared to model group, high, middle and low groups of Dragon's blood dropping pills could improve the neurological function significantly (pDragon's blood dropping pills had protective effects on focal cerebral ischemia rats.

  14. 羊角拗根的化学成分研究%Studies on Chemical Constituents in Roots of Strophanthus divaricatus

    Institute of Scientific and Technical Information of China (English)

    程纹; 王祝年; 王建荣; 晏小霞; 王茂媛

    2014-01-01

    应用柱色谱技术从羊角拗[Strophanthus divaricatus(Lour.) Hook.et Arn.]根的甲醇提取物中分离纯化出6个化合物,通过1H NMR和13C NMR等波谱技术并对照文献,鉴定其分别为(+)-pinoresinol(1)、沙门苷元(2)、17βH-沙门苷元(3)、羊角拗苷(4)、β-谷甾醇(5)和胡萝卜苷(6).其中,化合物2~4均为强心苷类化合物.化合物1和3为首次从该植物中分得.

  15. PORTUGUESE MODERNISM AND BRAZILIAN MODERNISM: WAS THERE A DIALOGUE? INTERVIEW WITH EDUARDO LOURENÇO

    Directory of Open Access Journals (Sweden)

    Madalena Vaz Pinto

    2015-04-01

    Full Text Available The interview with Eduardo Loureço now published, was made as a complement to my PHD thesis held in 2007 at Pontifícia Universidade Católica do Rio de Janeiro.  Entitled “Modernismos em língua desdobrada: Portugal e Brasil”, the work intended to discuss the relations between Portuguese and Brazilian modernism. Two interviews were made aiming a contemporary reflection about such an important period: one with Eduardo Lourenço and the other with Silviano Santiago. Eduardo Lourenço was chosen as a critical reference seemed obvious for his important writings on the subject, but also and above all, for his fearless perspective – frequently critical – in dealing with Portuguese culture. From the interview, I picked up the parts where the comparison between the two modernisms are the main subject. 

  16. 五种花灌木对低温胁迫的生理响应及抗寒性评价%Evaluation of cold resistance and physiological response to low temperature stress on five kinds of flowering shrubs

    Institute of Scientific and Technical Information of China (English)

    雷帅; 蒋倩; 汉梅兰; 张建旗; 朱亚灵; 牛伟丽; 韩艳; 邵金霞

    2015-01-01

    为了明确‘金叶锦带’(Weigela florida‘Gold rush’)、‘猬实’(Kolkwitzia amabilis Geaebn)、‘欧洲雪球’(Viburnum opulus)、‘大花圆锥绣球’(Hydrangea paniculata var.grandiflora Sieb.)、‘小紫珠’(Callicarpa di-chotoma (Lour)K.Koch)5种花灌木在兰州地区的越冬性与抗寒性,以一年生枝条为试材,采用梯度降温法,研究低温处理后枝条的生活力及其抗寒生理指标,并应用 Logistic 方程、隶属函数分析方法,对供试材料抗寒性进行了评价.结果表明:‘欧洲雪球’‘大花圆锥绣球’‘猥实’‘金叶锦带’‘小紫珠’的半致死温度(LT50)分别为-28.91、-26.08、-23.60、-22.66、-15.89℃;随着冷冻处理温度的降低,5种灌木相对电导率、丙二醛(MDA)含量、可溶性糖含量、游离脯氨酸含量持续升高,处理温度与相对电导率之间呈显著负相关.抗寒能力综合比较显示欧洲雪球抗寒性最强,小紫珠抗寒性最差;5种花灌木的抗寒性排序为:‘欧洲雪球’>‘大花圆锥绣球’>‘猬实’>‘金叶锦带’>‘小紫珠’.%In order to understand the overwintering performance and cold resistance of five kinds of flowering shrubs in winter of Lanzhou area,one-year old branches of Weigela florida'Gold rush'),Kolk-witziaamabilis Geaebn,Viburnumopulus,Hydrangeapaniculata Var.grandifloraSieb.,Callicarpadi-chotoma (Lour)K.Koch)were taken as materials,by using gradient cooling method to study the life force and related cold physiological characteristics of these branches.Logistic equation,membership function a-nalysis method were applied for evaluation the cold resistance.The results showed that semi-lethal temper-ature (LT50)of ‘Viburnumopulus’,‘Hydrangeapaniculata var.grandiflora Sieb.’,‘Kolkwitziaama-bilis Geaebn’,Weigela florida‘Gold rush’,‘Callicarpa dichotoma (Lour)K.Koch)’was -28.91 ℃,-26.08 ℃,-23.60 ℃,-22.66 ℃ and -15.89 ℃,respectively.With the freezing

  17. 皂角刺及其伪品同属植物棘刺的理化鉴别

    Institute of Scientific and Technical Information of China (English)

    周日宝; 石国强

    1999-01-01

    @@ 皂角刺属常用中药,来源于豆科Leguminosae)皂荚属(Gleditsia)植物皂荚(G.sinensis Lam.)的干燥棘刺,调查研究发现,在皂角刺原药材或饮片中常混杂有同属植物山皂荚(G.japonica Miq.)、华南皂荚(G.fera (Lour.)Merr.)、绒毛皂荚(G.vestita Chan et Hou ex B.G.Li)和滇皂荚(G.delavayi Franch.)的棘刺,类似的报道也较多[1,2].为此,笔者对皂角刺及其同属植物棘刺的混伪品进行了薄层层析和紫外吸收光谱的鉴别研究,现将结果报告如下.

  18. 黑胡椒荜澄茄与南五味子性状鉴别

    Institute of Scientific and Technical Information of China (English)

    戚秀萍; 侯沧

    2000-01-01

    @@ 黑胡椒,为胡椒科植物胡椒Piper nigrum L.的干燥近成熟果实.荜澄茄,为胡椒科植物荜澄茄Piper cubeba L.或樟科植物山鸡椒Litsea cubeba(Lour.)Pers.的干燥成熟果实.南五味子,为木兰科植物华中五味子Schisandra sphenanthera Rehd.et Wils.的干燥成熟果实.三者均为直径3~6 mm的近球形果实,表面颜色相近,极易混淆.

  19. HPLC estimation of berberine in Tinospora cordifolia and Tinospora sinensis

    Directory of Open Access Journals (Sweden)

    Srinivasan G

    2008-01-01

    Full Text Available A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd. Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour. Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80°. The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia .

  20. HPLC Estimation of berberine in Tinospora cordifolia and Tinospora sinensis.

    Science.gov (United States)

    Srinivasan, G V; Unnikrishnan, K P; Rema Shree, A B; Balachandran, Indira

    2008-01-01

    A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80 degrees ). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia. PMID:20390090

  1. A New Monoterpenoid and a New Flavonoid Glycoside from the Peels of Clausena lansium.

    Science.gov (United States)

    Deng, Hui-Dong; Cai, Cai-Hong; Liu, Shuai; Zeng, Yan-Bo; Mei, Wen-Li; He, Fan; Hua, Min; Dai, Hao-Fu; Li, Shao-Peng

    2016-05-01

    One new monoterpenoid, nerol oxide-8-carboxylic acid (1), and one new flavonoid glycoside, claulansoside A (2), together with six known compounds, clausenamide (3), quercetin (4), isorhamnetin (5), dihydromyric (6), 2",3"-dihydroxyanisolactone (7) and (E,E)-8-(7-hydroxy-3,7-dimethylocta-2,5-dienyloxy)psoralen (8), have been isolated from the peels of Clausena lansium (Lour.) Skeels. Their structures were determined using a combination of 1D, and 2D NMR (HMQC, HMBC, COSY and NOESY) techniques, and HR-ESI-MS analyses. Compounds 1 and 7 exhibited antibacterial activity against Staphylococcus aureus with the diameter of inhibition zones of 11.5 mm and 14.2 mm. Compounds 3 and 6 showed α-glucosidase inhibitory activity in vitro. PMID:27319119

  2. Soil Fertility in Agroforestry System of Chinese Fir and Villous Amomum in Subtropical China

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A trial of interplanting and non-interplanting villous amomum (Amomum villosum Lour.) under the canopy of Chinese fir (Cunninghamia lanceolata Hook.) at age 22 was established in Sanming, Fujian of China, and a survey on soil fertility was carried out 10 years after its establishment. Compared with the control (non-interplanting), the properties of soil humus in agroforestry system were ameliorated, with a higher level of humification and resynthesis of organic detritus. The soil microbial population and enzymatic activities were both higher under the influence of villous amomum. Both the nutrient supplying and nutrient conserving capacities of the soil were improved. This agroforestry system exhibited an advantage of improved soil fertility, as well as an accelerated growth of Chinese fir, it was, therefore, a sustainable management system suited for Chinese fir in South China.

  3. Clinical Evaluation Of A New Emollient Cream In Cry Skin Conditions

    Directory of Open Access Journals (Sweden)

    Shukla V A

    1998-01-01

    Full Text Available Dry skin is a common problem both in healthy individuates and in patients with skin diseases. In all cases, emollients play important role in rehydrating the stratum corneum. To evaluate the efficacy of a new emollient, thirty-six patients with symptomatic dry skin disorders were enrolled. They were given the test emollient cream for topical application twice daily for 3 weeks and followed up weekly. Subjective complaints included pruritus and discomfort while objective parameters included scaling, erythema, roughness of skin, fissuring and hypopigmentation. Treatment with the test emollient cream reduced the severity in a majority of patients. Moreover, being free of perfume, co lour, urea and lanolin, the test cream was well tolerated by all participants.

  4. Detection of DNA Hypomethylation Mediated Floral Induction in Longan and Spinach Using the HAT-RAPD Technique

    Directory of Open Access Journals (Sweden)

    S. Anuntalabhochai

    2009-01-01

    Full Text Available In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa, petunia (Petunia hybrida, spinach (Spinacea oleracea L. and longan (Dimocarpus longan Lour., which were treated using 5-azacytidine, potassium chlorate (KClO3 and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan.

  5. Molecular authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii by ITS and 5S rRNA spacer sequencing.

    Science.gov (United States)

    Sun, Ye; Shaw, Pang-Chui; Fung, Kwok-Pui

    2007-01-01

    In the present study, we examined nuclear DNA sequences in an attempt to reveal the relationships between Pueraria lobata (Willd). Ohwi, P. thomsonii Benth., and P. montana (Lour.) Merr. We found that internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA are highly divergent in P. lobata and P. thomsonii, and four types of ITS with different length are found in the two species. On the other hand, DNA sequences of 5S rRNA gene spacer are highly conserved across multiple copies in P. lobata and P. thomsonii, they could be used to identify P. lobata, P. thomsonii, and P. montana of this complex, and may serve as a useful tool in medical authentication of Radix Puerariae Lobatae and Radix Puerariae Thomsonii. PMID:17202681

  6. Effects of Pb stress on nutrient uptake and secondary metabolism in submerged macrophyte Vallisneria natans.

    Science.gov (United States)

    Wang, Chao; Lu, Jie; Zhang, Songhe; Wang, Peifang; Hou, Jun; Qian, Jin

    2011-07-01

    For better understanding the metabolic adaptations to Pb stress in submerged plants, the alterations in mineral elements uptake and in secondary metabolism were studied in leaves of Vallisneria natans (Lour.) Hara exposed to 0-100μM Pb for 0-7d. Pb content increased in leaves in a dose-dependent way. The increase of calcium, magnesium and iron content and the decrease of phosphorus, potassium and manganese content were detected in leaves of V. natans under Pb stress, while no significant changes were detected in copper and zinc concentration. Meanwhile, there was an increase in the concentrations of total phenolic and flavonoids. Pb treatment caused an increase in the catalytic activities of shikimate dehydrogenase, phenylalanine ammonialyase and polyphenol oxidase. The results suggest that nutrient uptake and secondary metabolism were actively regulated by V. natans plants in response to Pb stress. PMID:21440937

  7. GERMINATION EXPERIMENT ON SEEDS AND STEM TUBERS OF VALLISNERIA NATANS IN LAKE DONGHU OF WUHAN%武汉东湖苦草种子和块茎发芽实验

    Institute of Scientific and Technical Information of China (English)

    李亚东; 崔艳秋

    2000-01-01

    @@ 苦草[(Vallisneria natans(Lour.)Hara)]为多年生沉水草本,是长江中下游湖泊常见的沉水植物优势种.它既能有性繁殖,又能无性繁殖,无性繁殖体为带芽地下茎(当年萌发)和其末端膨大的越冬块茎[1,2].由苦草等种类组成的沉水植被是长江中下游湖泊主要的生物组份和重要的生物环境,对湖泊生态系统的结构和功能起关键作用[3,4].

  8. 鸡矢藤蚜Neoaulacorthum nipponicum (Essig & Kuwana)

    Institute of Scientific and Technical Information of China (English)

    陈静; 乔格侠

    2011-01-01

    鸡矢藤蚜Neoaulacorthum nipponicum(Essig&Kuwana)隶属于半翅目Hemiptera蚜科Aphididae长管蚜亚科Macrosiphinae,活体橘红色,头部背面、附肢及腹管黑色。寄主植物为茜草科Rubiaceae鸡矢藤属(PaederiaL.)植物,包括鸡矢藤(Paederia scandens(Lour.)Merr.,)毛鸡矢藤(Paederia tomentosa)(Paederia scandens var.mairei)和(Paederia scandens var.maritima),主要在叶片及茎上为害。主要分布于东亚地区,包括中国(四川、福建、台湾)、日本、韩国、泰国、爪哇、印度东北部等地区。主要鉴别特征:

  9. 大白菜黑斑病室内苗期抗性鉴定方法的研究%Study on Resistance Identification Method for Black Spot of Chinese Cabbage at Seedling Stage in Laboratory

    Institute of Scientific and Technical Information of China (English)

    甘彩霞; 祝花; 崔磊; 袁伟玲; 高翔; 符义彬; 梅时勇

    2012-01-01

    为了选育大白菜(Brassica campestris L.ssp pekinensis (Lour.) Olsson)抗黑斑病(A lternaria brassicae)新品种和种质创新,在室内利用6个品种的大白菜研究了接种物浓度、苗龄和温度对大白菜黑斑病发病的影响,结果表明,在(25±1)℃光照培养室内,选择浓度为1.0×104个孢子/mL或1.0×105个孢子/mL的黑斑病病菌孢子悬浮液喷雾接种于苗龄为3~4片真叶的大白菜幼苗,保湿24~48 h,10d后进行病情调查,可对大白菜种质资源抗黑斑病的抗性进行正确评价.%In order to provide technical support for breeding new varieties and germplasm innovation of Chinese cabbage (Brassica campestris L. ssp pekinensis(Lour.) Olsson) with resistance to black spot(Alternaria brassicae), the influence of inoculation spore concentration, seedling stage and temperature on black spot occurrence was studied with six varieties. It was showed that by spraying 1.0×104pfu/mL or 1.0×105pfu/mL spore suspension on seedling at 3~4 leaf stage and then moisturizing for 24~48 h at (25±1)℃ in door, the resistance of radish varieties on the black spot could be accurately evaluated 10 d after inoculation.

  10. The New Geographical Distribution Records of Wild Tree Species in Guangdong Province,China(II)%广东省野生树种地理分布新记录(续)

    Institute of Scientific and Technical Information of China (English)

    韩维栋; 陈杰

    2014-01-01

    近2年对广东省雷州半岛各地野生木本植物实地踏查与标本采集鉴定,并参照相关植物区系文献,发现广东省新分布野生树种5种1变种,分别为:琼刺榄( Xantolis longispinnsa( Merr.) H.S.Lo)、顶花杜茎山( Maesa balansae Mez)、海南山麻杆( Alchornea rugosa ( Lour.) Muell.Arg.var.pubescens ( Pax et Hoffm.) H.S.Kiu)、刺茉莉( Azima sarmentosa ( Bl.) Benth.et Hook.f.)、单叶拿身草( Desmodium zonatum Miq.)、过山崖爬藤( Tetrastigma pseudocruciatum C.L.Li)。%For the last two years ,based on the related flora references and the author′s field survey in Leizhou Peninsula of Guang-dong Province,China,there were 5 species and 1 variety found as new wild tree species records of their geographical distribution in Guangdong Province.They were Xantolis longispinnsa ( Merr.) H.S.Lo, Maesa balansae Mez., Alchornea rugosa ( Lour.) Muell.Arg.var.pubescens (Pax et Hoffm.)H.S.Kiu,Azima sarmentosa(Bl.) Benth.et Hook.f.,Desmodium zonatum Miq.,Tet-rastigma pseudocruciatum C.L.Li.

  11. Bioprospecting insecticidal compounds from plants native to Mato Grosso do Sul, Brazil Bioprospecção de substâncias inseticidas de plantas nativas de Mato Grosso do Sul, Brasil

    Directory of Open Access Journals (Sweden)

    Antonio P. Souza

    2008-12-01

    Full Text Available This paper reports on an evaluation of the insecticidal activity of extracts prepared from leaves of Tapirira guianensis Aubl. (Anacardiaceae, Attalea phalerata (Mart. ex Spreng. Burret (Arecaceae, Eugenia uniflora L. (Myrtaceae, and Gomphrena elegans Mart. (Amaranthaceae and from stems of Myracrodruon urundeuva Allemão (Anacardiaceae. Four extracts and 18 fractions with a range of polarities were tested. Ten-gram batches of wheat grains were each nebulized with 1 mL of a separate extract at 10% w/v. After solvent evaporation at 38 ºC, the grains were placed into flasks along with 20 unsexed 10- to 20-day old adult individuals of Sitophilus zeamais. The assessment was carried out on the fifth and tenth day by counting and discarding the dead insects. Leaf extracts of G. elegans showed an insecticidal effect ranging from 27% to 60% by the fifth day, whereas the effect of the remaining extracts tested (if active at all did not exceed 20% in the same period. By the tenth day, the most active extracts were those of A. phalerata (hexanic, 36.5% and all those of G. elegans (5280.5%, whereas the effect of the other extracts did not exceed 30%.No presente trabalho foi avaliada a atividade inseticida de extratos de limbos foliares de Tapirira guianensis Aubl. (Anacardiaceae, Attalea phalerata (Mart. ex Spreng. Burret (Arecaceae, Eugenia uniflora L. (Myrtaceae e Gomphrena elegans Mart. (Amaranthaceae e de caules de Myracrodruon urundeuva Allemão (Anacardiaceae. Foram testados quatro extratos e 18 frações, com diferentes polaridades. Dez gramas de grãos de trigo foram pulverizados com 1 mL de cada extrato a 10% (p/v. Após a evaporação do solvente a 38 ºC, os grãos foram acondicionados em recipientes juntamente com 20 indivíduos adultos de Sitophilus zeamais não sexados, com 10 a 20 dias de idade. As avaliações foram feitas no quinto e no décimo dia, contando-se o número de insetos mortos e descartando-os. Os extratos foliares de G. elegans

  12. Fungi Associated with Capororoca (Myrsine ferruginea Myrsinaceae Fruits and Seeds Fungos Associados aos Frutos e Sementes de Capororoca (Myrsine ferruginea Myrsinaceae

    Directory of Open Access Journals (Sweden)

    Suelen Santos Rego

    2010-02-01

    Full Text Available The objectives of this study were to identify the fungi associated with capororoca (Myrsine ferruginea Spreng fruits and seeds from different lots and check the incidence of fungi between green and ripe fruits and
    seeds from these fruits. For fungi detection Blotter test (BT method was utilized, being 400 fruits and 400 seeds each sample, and incubated at 20 ºC ±1 ºC, 12 h dark light/12 h complete dark, for seven days, followed by the evaluation of the fungi incidence (%. It was possible to verify the transmission of the fungus Alternaria sp., Cladosporium sp., Colletotrichum sp., Fusarium sp., Macrophomina sp. and Pestalotia sp. fruit for seeds. Evaluated in three lots, Pestalotia sp. had the highest percentage of incidence in the seeds. The fungal contamination in fruits were higher than in seeds, indicating that the seed treatment reduces the appearance of fungi. There were differences in the occurrence of fungi among the lots evaluated, and the lot from Colombo County had higher incidence of fungi.

    doi: 10.4336/2009.pfb.58.87

    Os objetivos neste trabalho foram identificar os fungos associados aos frutos e sementes de capororoca (Myrsine ferruginea Spreng obtidos de diferentes lotes e verificar a incidência de fungos entre frutos verdes e maduros e nas sementes obtidas destes frutos. Para a detecção de fungos utilizou-se o método de papel-filtro (PF, sendo 400 frutos e 400 sementes para cada amostra, incubadas sob 12 horas luz negra/12 horas escuro, na temperatura de 20 ºC ± 1 ºC durante sete dias, avaliando-se a incidência (% de fungos. Pôde-se verificar a possível transmissão dos fungos Alternaria sp., Cladosporium sp., Colletotrichum sp., Fusarium sp., Macrophomina sp. e Pestalotia sp. dos frutos para as sementes. Nos três lotes avaliados, Pestalotia sp. apresentou a maior percentagem de incidência nas sementes. A contaminação fúngica nos frutos foi maior que nas sementes, indicando que o

  13. Potencial alelopático de espécies nativas na germinação e crescimento inicial de Lactuca sativa L. (Asteraceae Allelopathic potential of native species in Lactuca sativa L. (Asteraceae germination and initial growth

    Directory of Open Access Journals (Sweden)

    Fabiana Maraschin-Silva

    2006-03-01

    Full Text Available A alelopatia caracteriza-se pelos efeitos danosos ou benéficos sobre o desenvolvimento da vegetação, causados por substâncias químicas produzidas e liberadas para o ambiente por uma planta. Com o objetivo de avaliar o potencial alelopático de espécies brasileiras, foram testados extratos foliares de Cecropia pachystachya Trec. (Urticaceae, Peltophorum dubium (Spreng. Taub. (Fabaceae, Psychotria leiocarpa Cham. & Schltdl (Rubiaceae, Sapium glandulatum (Vell. Pax (Euphorbiaceae e Sorocea bonplandii (Baill. Burg., Lanj. & Boer (Moraceae, utilizando-se bioensaios de germinação e crescimento e alface (Lactuca sativa L. como planta alvo. Nesses bioensaios, foram usados extratos foliares aquosos nas concentrações de 2 e 4%, preparados por maceração estática com água fria e quente. Os extratos das cinco espécies causaram atraso na germinação dos aquênios da alface, bem como efeitos tóxicos no crescimento das plântulas, com redução e enfraquecimento das raízes. Os resultados obtidos mostraram a presença de substâncias químicas inibidoras nos extratos, revelando potencial alelopático para as cinco espécies avaliadas.Allelopathy is characterized by harmful or beneficial effects on vegetation development, caused by chemical substances produced and released into the environment by the plant. Aiming to assess the allelopathic potential of Brazilian species, aqueous leaf extracts of Cecropia pachystachya Trec. (Urticaceae, Peltophorum dubium (Spreng. Taub. (Fabaceae, Psychotria leiocarpa Cham. & Schltdl (Rubiaceae, Sapium glandulatum (Vell. Pax (Euphorbiaceae, and Sorocea bonplandii (Baill. Burger, Lanj. & Boer (Moraceae were tested on lettuce using germination and growth bioassays. In these bioassays, aqueous leaf extracts were used at concentrations of 2 and 4%, prepared by static maceration with cold and hot water. The five species extracts delayed lettuce germination and produced toxic effects on seedling growth, with root

  14. Ambientes de ocorrência e flora acompanhante do gênero Himatanthus em Alcântara, Maranhão, Brasil Occurrence environments and accompanying vegetation of genus Himatanthus in Alcântara, Maranhão, Brazil

    Directory of Open Access Journals (Sweden)

    J.F.P. Linhares

    2011-01-01

    Full Text Available A janaúba (Himatanthus spp., ocorre em populações naturais em ecossistemas florestais e apresenta amplo espectro de usos na medicina popular que vai desde o tratamento de inflamações uterinas, gastrite, uso veterinário, complemento alimentar, até tratamento de câncer. O extrativismo de látex de janaúba em Alcântara vem adquirindo importância crescente como alternativa de renda. Sendo assim, o estabelecimento de estudos que viabilizem o manejo sustentado em seu ambiente natural é necessário. O objetivo deste trabalho foi de caracterizar os ambientes de ocorrência de Himatanthus no município de Alcântara, Maranhão, Brasil, e identificar a flora acompanhante. As amostragens foram definidas por indicação de informantes, e aparente frequência na comunidade vegetal; os ambientes foram descritos considerando as unidades de paisagem e histórico de uso. Como resultados, a maior área de ocorrência foi a terra firme seguida por várzeas de restinga; a principal tipologia vegetacional foi a mata secundária. O bacuri (Platonia insignis Mart., tucum (Astrocaryum vulgare Mart. e a murta verdadeira (Myrcia selloi (Spreng. N. Silveira, foram às principais espécies associadas.The Frangipani (Himatanthus spp. occur in natural populations in forest ecosystems and present a wide spectrum of uses. in folk medicine ranging from the treatment of uterine inflammation, gastritis, veterinary, food supplement to medicinal treatment of cancer. The extraction of latex Janaúba Alcantara has been gaining increasing importance as an alternative income. Therefore, the establishment of studies that enable sustainable management in their natural environment is necessary. The overall objective of this study was to characterize the occurrence of Himatanthus environments in the municipality of Alcântara, Maranhão, Brazil, and identify the accompanying vegetation. The samples were defined by word of informants, and apparent frequency in the plant

  15. Reforestation of riparian areas by direct seeding: the effects of sazonality, soil use, exclusion of predation, and depth on initial survival/ Semeadura direta na recomposição vegetal de áreas ciliares: efeitos da sazonalidade, uso do solo, exclusão da predação, e profundidade na sobrevivência inicial

    Directory of Open Access Journals (Sweden)

    M.M Malavasi

    2005-06-01

    Full Text Available This study reports the effects of seasonality, depth of seeding, and predation exclusion on the early survival of Peltophorum dubium (Spreng. Taub. and Enterolobium contortisiliquum (Vell. Morong. seeds used to re-vegetate riparian areas. The study used proprieties along Guavira stream, in the west Parana region. Direct seeding was performed in the second week of spring, summer, autumn, and winter. Seeds were placed at the soil surface or at 2 cm depth. Predation exclusion was accomplish by a metal cage. The spring and summer seeding had survival rates of 36.6 % and 25.5 % in areas used for grain or pasture production and only 9.73 % for areas under forest patches when seeded at 2 cm depth. In general, during all seasons germinant survival was higher in altered areas than under forest patches.O ensaio testou os efeitos da sazonalidade, uso do solo, profundidade de semeadura e exclusão da predação na sobrevivência após 90 dias da semeadura direta de canafístula (Peltophorum dubium (Spreng. Taub. e de timburi (Enterolobium contortisiliquum (Vell. Morong. em áreas de domínio ciliar alteradas antropicamente. Foram efetuadas semeaduras na primavera (setembro, e verão (dezembro de 2002, assim como no outono (março, e inverno (junho de 2003 sempre na segunda semana após o início da estação, na superfície e à profundidade de 2 cm, com e sem a exclusão da predação. O ensaio foi implantado em propriedades rurais limítrofes ao riacho Guavirá, oeste do Paraná. Quando semeadas a profundidade de 2 cm na primavera e verão, as sementes das duas espécies apresentaram sobrevivência de 36,6 % e 25,5 % para áreas com produção de grãos e pastagem, respectivamente, e de 9,73 % para áreas sob remanescentes arbóreos. A sobrevivência de germinantes após 90 dias, em áreas de domínio ciliar utilizada para produção tanto de grãos como de pasto, foi maior do que aquela sob a proteção de remanescentes arbóreos.

  16. Aspectos de la biología de un hongo del género Rhizoctonia y de su interacción in vitro con Fusarium oxysporum f. sp. dianthi. dianthiBiological aspects of one fungi Rhizoctoniaand its in vitrointeraction withFusarium oxysporum f. sp.dianthi

    Directory of Open Access Journals (Sweden)

    Peñuela Angélica

    2002-06-01

    Full Text Available Se estudiaron diferentes aspectos de la biología de un hongo aislado de Theobromagrandiflorum(Spreng K. Schum, tales como su comportamiento como antagonista encondiciones in vitrocon Fusarium oxysporum Schlencht f. sp. dianthi(Prill y Del Snyder yHansen. Se encontró que el hongo cultivado en PDA, condiciones de microcultivo y luznatural a 20ºC crece lentamente y produce abundantes filamentos monilioides; a 27ºCy 30ºC, crece rápidamente. En condiciones de oscuridad a 20ºC, los resultados sonsimilares a los obtenidos a 30ºC en luz natural. Al término de un mes, el hongo produceesclerocios. En AMS, en cajas de Petri, luz natural y a 20ºC, se observan "sectores" muybien delimitados, lo que indica una fuerte y evidente variabilidad genética, demostradaen cambios fenotípicos. En AAg, el hongo crece pobremente. Los resultados obtenidosindican que en PDA, 30ºC y condiciones de oscuridad, este hongo, Rhizoctonia sp., crecerápida y vigorosamente, produciendo abundantes conidias en sus esclerocios. Con rela-ción a su comportamiento in vitrofrente a Fusarium oxysporumScht. f. sp. dianthi, Rhizoctoniaspcrece con mayor rapidez y agresividad que Fusarium oxysporum, llegando a inhibirlo.ABSTRACTGrowth in different culture media and temperature as well as its antagonic roleagainst Fusarium oxysporumSchlencht f. sp. dianthi(Prill & Del Snyder & Hansen, werestudied on an isolated of the fungi Theobroma grandiflorum(Spreng K. Schum. Lowgrowth and the presence of abundat moniloid cells were detected when the fungi wascultured in PDA in microculture, with natural light and at 20ºC. Higher temperatures(27ºC and 30ºC increased growh rate. When cultured at 20ºC, in darkness, similarresults as those recorded at 30ºC in natural light were found. After a month sclerotiagrotwh was present. Culture in AMS in Petri dishes, with natural light at 20ºC showedgrowth in patches which suggests phenotype variability. Poor growth was recordedwhen cultured in AAg

  17. COCHINCHINENIN—A NEW CHALCONE DIMER FROM THE CHINESE DRAGON BLOOD%剑叶血竭素国产血竭中一个新的二聚查耳酮

    Institute of Scientific and Technical Information of China (English)

    周志宏; 王锦亮; 杨崇仁

    2001-01-01

    目的研究国产商品血竭的活性成分。方法用甲醇提取,Sephadex L-20, MCI gel和硅胶柱色谱进行分离纯化,用现代波谱学方法进行结构鉴定。结果从国产雨林牌血竭的甲醇浸提物中得到9个查耳酮化合物,分别鉴定为:1-[5-(2,4,4′-三羟基二氢查耳酮基)]-1-对羟基苯基-3-(2-甲氧基-4-羟基苯基)-丙烷(1), 2′-methoxysocotrin-5′-ol (2), socotrin-4′-ol (3), 2-甲氧基-4,4′-二羟基二氢查耳酮(4), 2,4,4′-三羟基二氢查耳酮(5), 2,4,4′-三羟基-6-甲氧基二氢查耳酮(6), 2′,4′,4-三羟基查耳酮(7), 2-甲氧基-4,4′-二羟基查耳酮(8), 2′-甲氧基-4′,4-二羟基查耳酮(9)。结论 1为一新的查耳酮二聚体,命名为剑叶血竭素(cochinchinenin),2-9为首次从该血竭中分离得到。%AIM To study the active constituents of Dracaena cochinchinensis (Lour.) S.C.Chen. in the commercial dragon blood. METHODS Various column chromatographies with Sephadex L-20 gel, MCI gel and silica gel were employed for the isolation and purification. The structures of compounds were elucidated by spectral analysis. RESULTS Nine chalcones were isolated from the commercial dragon's blood which was made of D.cochinchinensis (Lour.) S.C.Chen.. By means of spectral data, they were identified as 1-[5-(2,4,4′-trihydroxydihydrochalconyl)]-1-(p-hydroxyphenyl)-3-(2-methoxy-4-hydroxy-phenyl)-propane (1), 2′-methoxysocotrin-5′-ol (2), socotrin-4′-ol (3), 2-methoxy-4,4′-dihydroxydihydrochalcone (4), 2,4,4′-trihydroxy-dihydrochalcone (5), 2,4,4′-trihydroxy-6-methoxydihydrochalcone (6), 2′,4′,4-trihydroxy-chalcone (7), 2-methoxy-4,4′-dihydroxychalcone (8) and 2′-methoxy-4′,4-dihydroxychalcone (9). CONCLUSION Compound 1 is a new chalcone dimer and named as cochinchinenin. Compounds 2-9 were isolated from D.cochinchinensis (Lour.) S.C.Chen. for the first time.

  18. Optimization of AFLP reaction system in Canarium album%橄榄基因组AFLP扩增体系的优化

    Institute of Scientific and Technical Information of China (English)

    王燕平; 陈勤; 周志钦; 周俊辉

    2012-01-01

    为建立适于橄榄研究用的AFLP技术体系,以10个橄榄品种为材料,对其基因组DNA从酶切、连接、预扩增和选择性扩增等方面进行条件优化。研究结果表明,DNA模板用量为500ng,酶切体系中限制性内切酶EcoRⅠ和MseⅠ各加入3U,37℃水浴4h;连接体系中T4 DNA连接酶加入1.5U,16℃连接6h;并利用优化后的预扩增体系和选择性扩增体系筛选出6对适合于橄榄AFLP分析的引物组合:E/AAC—M/CAT、E/AAC—M/CTT、E/ACA—M/CAA、E/AGG—M/CAA、E/ACC—M/CAA、E/ACT—M/CAT。采用该体系得到的电泳条带清晰可靠、多态性好,可用于对橄榄遗传多样性的研究。%To establish an appropriate AFLP(Amplifiied Fragment Length Polymorphism) analysis system for Canariurn album (Lour.) Raeusch, 10 cuhivars of C. album (Lour.) Raeusch were involved in optimization of enzyme digestion, ligation, pre-amplification and selective amplification. The results suggested that about 500 ng DNA should be digested with 3 units of EcoR I and Mse I enzymes respectively in a reaction volume of 20 μL, and incubated at 37 ℃ for 4 h. Double-stranded adaptors were legated to the restriction fragments with 1.5 units of T4DNA ligation enzyme at 16 ℃ for 6 h. With the optimized pre-amplifieation and selective amplification systems, 6 pairs of primers (E/AAC-M/CAT, E/AAC-M/CTT, E/ ACA-M/CAA, E/ACC-M/CAT, E/ACC-M/CAA, E/ACT-M/CAT) were selected for the AFLP reaction system of Canarium album. The clear and highly polymorphic AFLP bands could be obtained by this reaction system, which was useful for the further research on genetic diversity of Canarium album in Guangdong province.

  19. Researches on Physiological and Biochemical Indexes of Drought Resistance Stress of Three Ornamental Bamboos%3种观赏竹抗旱性生理生化指标研究

    Institute of Scientific and Technical Information of China (English)

    陈羡德; 陈珺

    2014-01-01

    研究了干旱胁迫条件下,凤尾竹、孝顺竹、四季竹等3种观赏竹叶片的游离脯氨酸含量、可溶性糖含量,超氧岐化酶(SOD)以及过氧化氢酶(CAT)的活性变化。结果表明:随着干旱胁迫时间的延长,3个竹种叶片脯氨酸含量呈增加趋势,叶片可溶性糖含量呈先升后降趋势,而叶片超氧岐化酶( SOD)以及过氧化氢酶( CAT)活性总体上均呈下降趋势。根据4项生理指标,采用模糊隶属函数法对其抗旱性进行综合评价,抗旱能力强弱顺序为:四季竹>凤尾竹>孝顺竹。%The contents of free proline , soluble sugar and SOD activity , CAT activity of three bamboo leaves ( B.multiplex cv.Fernleaf,B.multiplex (Lour.) Raeuschelex J,S.tootsik (Sieb.) Makino ) were studied under natural drought stress condi-tions.The results showed that free proline contents of bamboo leaves increased ,the soluble sugar content increased at first and then decreased ,SOD activity and CAT activity decreased with the prolongation of the stress time.The method of obscure mathematics was used to evaluate their drought resistance ability comprehensively based on physiological and biochemical indexes .The ability of drought resistance indicated that S.tootsik ( Sieb.) Makino>B.multiplex cv.Fernleaf>B.multiplex ( Lour.) Raeuschelex J accord-ing to the results of fuzzy administering function.

  20. Primary Report on Resistance and Absorption of Mulberry to SO2 and Fluoride in Atmosphere%桑树对大气中二氧化硫和氟化物的抗性及吸收能力研究

    Institute of Scientific and Technical Information of China (English)

    吴洪丽; 郝瑜; 刘岚; 许淑琼; 叶楚华; 孙波; 周洪英; 叶建美

    2015-01-01

    Sulfite and Fluoride content in leaves of Morus alba L. cv. Nongsang 14, Lagerstroemia indica L, L. indica L. f. alba (Nichols.) Rehd., Quercus acutissima Carruth., and Osmanthus fragrans(Thunb.)Lour. var. aurantiacus Makino in the test point of different pollution level were tested. The result showed that fluoride in M. alba cv. Nongsang 14 is significantly high-er than in others; and with the increasing of atmospheric fluoride pollution, more fluoride was fixed. While the absorption of M. alba cv. Nongsang 14 to SO2 is similar to other plants. SEM result shows leaf surface of M. alba cv. Nongsang 14 is rich of deep wrinkles where particles gathered together, indicating strong adsorptivity to pollution such as dust in atmosphere.%对在大气污染程度不同环境里生长的农桑14(Morus alba L. cv. Nongsang 14)、紫薇(Lagerstroemia indica L.)、银薇[Lagerstroemia indica L. f. alba (Nichols.) Rehd.]、栎树(Quercus acutissima Carruth.)、丹桂[Osmanthus fragrans (Thunb.)Lour. var. aurantiacus Makino]5种植物叶片中的亚硫酸盐和氟化物含量进行了测定与分析,发现农桑14对二氧化硫的吸收与其他4种对照植物相比没有明显优势,但对氟化物的吸收能力极显著高于其他4种对照植物,且随着空气中氟化物浓度的升高,吸收量也明显增高。通过电子显微镜扫描观察发现,与其他4种对照植物相比,桑叶表面的皱褶深凹,集聚了较多的颗粒状物,表明农桑14对空气中粉尘等污染物具有较强的吸附能力。

  1. Ploidia e fertilidade de pólen em progênies de citros Ploidy and pollen fertility in citrus hybrids

    Directory of Open Access Journals (Sweden)

    Eduardo Cesar Brugnara

    2008-01-01

    Full Text Available Este trabalho foi desenvolvido para estimar a fertilidade do pólen e determinar o nível de ploidia em progênies dos cruzamentos da tangerineira 'Montenegrina' (C. deliciosa Ten. com a tangerineira 'King' (C. nobilis Lour. e com a laranjeira 'Caipira' (C. sinensis (L. Osb.. As plantas, de pés-francos e com idade entre 11 e 12 anos, são mantidas na Estação Experimental Agronômica da Universidade Federal do Rio Grande do Sul, em Eldorado do Sul. Avaliaram-se o nível de ploidia, através da contagem dos cromossomos em células em meiose, e a fertilidade do pólen, por coloração do pólen com carmim propiônico, em 2005 e 2006. Todos os híbridos avaliados são diplóides e a fertilidade de pólen variou de zero a 98%. C27 - híbrido de 'Montenegrina' x 'Caipira' - revelou-se praticamente estéril, e a fertilidade de C21, do mesmo cruzamento, é de 10%. A menor fertilidade observada no cruzamento da 'Montenegrina' x 'King' foi de 42%.This work was performed to estimate pollen fertility and determinate the ploidy level of 11 to 12 years old progenies of crosses of 'Montenegrina' mandarin (Citrus deliciosa Ten. with 'King' mandarin (C. nobilis Lour. and with 'Caipira' sweet orange (C. sinensis (L. Osb. maintained at Estação Experimental Agronômica of UFRGS, in Eldorado do Sul, Brazil. The ploidy level was determined by chromosome countings in meiosis cells and pollen fertility evaluated by staining pollen grains with propionic carmine. All evaluated hybrids are diploid and pollen fertility varied from zero to 98%. C27, a hybrid 'Montenegrina' x 'Caipira', was found is sterile, and C21, from the same cross, showed 10 % fertility. The lowest observed fertility in the progeny 'Montenegrina' x 'King' was 42 %.

  2. Musgos urbanos do recanto das Emas, Distrito Federal, Brasil

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    Câmara Paulo Eduardo

    2003-01-01

    Full Text Available Fruto da ocupação desordenada de Brasília, o Recanto das Emas é uma das mais recentes cidades criadas no entorno da capital. Localizada a 25,8km do Plano Piloto, conta com área de 101.476km². O Recanto das Emas foi criado em 1993 e hoje conta com população de aproximadamente 100.000 habitantes. Foram selecionados vários pontos de coleta procurando abranger toda a cidade e diferentes áreas. Foram encontrados relativamente poucos representantes da brioflórula, com predomínio de espécies ruderais. A divisão Bryophyta conta com 10 espécies e sete famílias, destacandose a família Bryaceae com três espécies. Barbula indica (Hook. Spreng. é citada pela primeira vez para a região Centro-Oeste. Os resultados explicam-se pelo pouco tempo de existência da cidade, que conta com poucos substratos para fixação das populações de briófitas e não permitiram ainda o estabelecimento de nova cobertura vegetal.

  3. New records of Pteridophytes for Kashmir Valley, India

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    SHAKOOR A. MIR

    2014-10-01

    Full Text Available Mir SA, Mishra AK, Reshi ZA, Sharma MP. 2014. New Records of Pteridophytes for Kashmir Valley, India. Biodiversitas 15: 131-136. During the recent field survey of district Shopian four species of Pteridophytes are reported for the first time that constitutes new records for Kashmir valley. These species are Hypolepis polypodioides (Blume Hook, Pteris stenophylla Wall. ex Hook. & Grev., Dryopteris subimpressa Loyal and Dryopteris wallichiana (Spreng. Hylander. The diagnostic features of H. polypodioides are presence of long-creeping slender rhizome and eglandular, colorless or brown tinged hairs throughout the frond. P. stenophylla is characterized by having dimorphic fronds and 3 to 5 pinnae clustered at stipe apex. D. subimpressa is marked by pale-green lamina and the largest basiscopic basal pinnule in the lowest pair of pinnae. Similarly, the characteristic features of D. wallichiana are presence of huge frond size, glossier and dark-green lamina and dense browner scales in stipe and rachis. In present communication taxonomic description, synonyms, ecology and photographs are provided for each of these newly recorded species.

  4. Molecular cloning and characterization of a gene encoding RING zinc finger ankyrin protein from drought-tolerant Artemisia desertorum

    Indian Academy of Sciences (India)

    Xiuhong Yang; Chao Sun; Yuanlei Hun; Zhongping Lin

    2008-03-01

    A RING zinc finger ankyrin protein gene, designated AdZFP1, was isolated from drought-tolerant Artemisia desertorum Spreng by mRNA differential display and RACE. Its cDNA was 1723 bp and encoded a putative protein of 445 amino acids with a predicted molecular mass of 47.9 kDa and an isoelectric point (pI) of 7.49. A typical C3HC4-type RING finger domain was found at the C-terminal region of the AdZFP1 protein, and several groups of ankyrin repeats were found at the N-terminal region. Alignments of amino acid sequence showed that AdZFP1 was 66% identical to the Arabidopsis thaliana putative RING zinc finger ankyrin protein AAN31869. Transcriptional analysis showed that AdZFP1 was inducible under drought stress in root, stem and leaf of the plant. Semi-quantitative reverse-transcriptase-polymerase chain reaction (RT-PCR) analysis showed that the transcript of AdZFP1 was strongly induced by exogenous abscisic acid (ABA) and also by salinity, cold and heat to some extent. Overexpression of the AdZFP1 gene in transgenic tobacco enhanced their tolerance to drought stress.

  5. Vasoactive and antioxidant activities of plants used in Mexican traditional medicine for the treatment of cardiovascular diseases.

    Science.gov (United States)

    Ibarra-Alvarado, C; Rojas, A; Mendoza, S; Bah, M; Gutiérrez, D M; Hernández-Sandoval, L; Martínez, M

    2010-07-01

    This study demonstrated that the aqueous extracts of plants employed in Mexican traditional medicine for the treatment of cardiovascular diseases are able to modify the tone of arterial smooth muscle. Agastache mexicana (Kunth) Lint & Epling (Labiatae), Chenopodium murale L. (Chenopodiaceae), Chirantodendron pentadactylon Larreat (Sterculiaceae), Dracocephalum moldavica L. (Labiatae), Psittacanthus calyculatus G. Don (Loranthaceae), Prunus serotina ssp. capuli (Cav. ex Spreng) McVaugh (Rosaceae), and Sechium edule Sw. (Cucurbitaceae) contain secondary metabolites that promote vascular relaxation and display antioxidant activities. As expected, their antioxidant effects showed a significant correlation with the polyphenolics content. However, a lower correlation was found between the antioxidant activity and the maximum vasodilatory effect, suggesting that the vasodilatation elicited by the plant extracts could be only partly attributed to their antioxidant properties. The extract of P. calyculatus, which displayed a maximum vasorelaxant effect that was higher than that of acetylcholine, induced endothelium-dependent vasodilatation. Futhermore, the vasorelaxant response to the P. calyculatus extract was reduced after adding an inhibitor of soluble guanylate cyclase activity, providing evidence that the NO/cGMP pathway is involved. On the other hand, the extracts of Bocconia frutescens L. (Papaveraceae), Magnolia grandiflora L. (Magnoliaceae), and Solanum rostratum Dunal (Solanaceae) induced concentration-dependent contraction of rat aortic rings, suggesting that these plants have potential health benefits for the treatment of ailments such as venous insufficiency. The pharmacological activities of the extracts studied provide scientific support for their ethnomedical use. PMID:20645769

  6. Plant regeneration via somatic embryogenesis from protoplasts of six plant species related to Citrus.

    Science.gov (United States)

    Jumin, H B; Nito, N

    1996-01-01

    Protoplasts isolated from embryogenic callus of Fortunella polyandra (Ridl.), Atalantia bilocularis (Pieree ex Guill.), Hesperethusa crenulata (Roxb.), Glycosmis pentaphylla (Retz.) Corr., Triphasia trifolia (Burm. f.) P. Wils. and Murraya koenigii (L.) Spreng. were cultured in MT (Murashige and Tucker 1969) basal medium containing 5% sucrose supplemented with 0.0, 0.001, 0.01, 0.1 or 1.0 mg l(-1) BA and 0.6 M sorbitol. The highest plating efficiencies for all species were obtained on MT basal medium containing 5% sucrose supplemented with 0.001 mg l(-1) BA. F. polyandra produced higher percentages of globular somatic embryo development, while A. bilocularis consistently showed a lower percentage of globular somatic embryo development in all 5 concentrations of BA. MT basal medium containing 5% sucrose and supplemented with 0.001 mg l(-1) BA was found to be a suitable medium for development of globular somatic embryos derived from protoplasts to form heart-shaped somatic embryos with cotyledon-like structures. The highest percentages of shoot formation for all 6 species were obtained using 0.1 mg l(-1) GA3. A complete protoplast-to-plant system was developed for F. polyandra, A. bilocularis and T. trifolia, which could facilitate the transfer of nuclear and cytoplasmic genes from these species into cultivated Citrus through protoplast fusion. PMID:24178352

  7. Floristic and phytosociological investigation of trees in a seasonal deciduous forest fragment at São Miguel do Oeste, Santa Catarina

    Directory of Open Access Journals (Sweden)

    Geniane Schneider

    2014-06-01

    Full Text Available The forests in Santa Catarina have been reduced and fragmented, and seasonal deciduous forests are among the most afflicted. The aim of the investigation was to describe arboreal composition and structure in a seasonal deciduous forest fragment. We established ten sample units of 20x20 m, covering a total area of 0.4 ha. Trees with circumference at breast height (CBH ≥ 25 cm were identified, and species richness and diversity were evaluated. A total of 205 individuals was sampled from 54 species, representing 44 genera and 23 families. Tree diversity was estimated to be 3.472 (nats using the Shannon-Weiner index (H’, and Pielou’s evenness (J’ was estimated at 0.87. The families with the largest number of species were Fabaceae, Myrtaceae, Meliaceae, Lauraceae and Sapindaceae. The species with the highest importance and coverage values were Nectandra megapotamica (Spreng. Mez, Sorocea bonplandii (Baill. W.C. Burger, Lanjouw & Boer, and Ocotea diospyrifolia (Meisn. Mez. Species richness and diversity are within the range of values cited for seasonal deciduous forests in the Floristic Forest Inventory of Santa Catarina, which shows the impact of exploration and fragmentation on this habitat. Nevertheless, the studied area has apparently conserved a significant portion of the tree species cited for this forest type.

  8. Persistência de plantas medicinais em sistemas agroflorestais no município de São Bento do Sul, SC, Brasil

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    A.L. Hanisch

    2013-01-01

    Full Text Available No final da década de 90, a comunidade de Rio Natal, no município de São Bento do Sul, SC, passou a fazer parte de uma Área de Preservação Ambiental e desde então, muitas áreas de cultivo, paulatinamente, estão retomando o processo de formação florestal. Neste cenário, foi sugerido pela comunidade, a possibilidade de cultivo de plantas medicinais adaptadas às condições de sombreamento. Em atendimento a essa demanda foi realizada uma pesquisa participativa na comunidade, de 2005 a 2008, onde foram avaliadas 14 espécies de plantas medicinais com potencial de mercado, cultivadas em áreas sombreadas, em três propriedades familiares. Foram avaliados: taxa de sobrevivência das espécies, incidência de doenças e pragas, teor de massa seca e altura das plantas. Apresentaram persistência ao cultivo em áreas sombreadas as espécies: Curcuma longa L. (açafrão-da-índia; Arctium lappa L. (bardana; Pfaffia glomerata (Spreng Pedersen (fáfia ou ginseng-brasileiro; Mikania glomerata Sprengel (guaco; Alternanthera brasiliana L. Kuntze (penicilina; Pogostemon cablin (Blanco Benth (patchuli e Maytenus ilicifolia Mart. Ex Reissek (espinheira-santa.

  9. Teste de especificidade hospedeira de Phaedon confinis (Coleoptera, Chrysomelidae, um potencial agente de biocontrole de Senecio brasiliensis (Asteraceae.

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    Julianne Milléo

    2011-07-01

    Abstract. Senecio brasiliensis (Spreng. Less when ingested by cattle and horses, the plant causes seneciosis, a serious poisoning. Due to the great financial losses to cattle ranchers, controlling the plant using insects has become attractive. Systematic survey efforts have revealed that Phaedon confinis Klug causes serious damage to the plant, and may be a great biocontrol agent. The object was to extend the tests of host specificity to 52 plants using 1st larval instar and adult chrysomelid bettles. The insects were submitted to “no-choice” and “multiple-choice” tests. The following results were obtained: “NO-CHOICE” L1 – 52 plants tested: null 90.39%; negligible damage 5.77%; light 1.92%; and normal in only S. brasiliensis 1.92%, where 31.67% of larvae obtained an adult phase. “NO-CHOICE” ADULTS – 46 plants. Null damage was recorded in 82.60%; 13.04% showed negligible damage; 2.17% light; 2.17% normal in S. brasiliensis. The chysomelids oviposited during observation days only on S. brasiliensis leaves. 615 eggs were oviposited with 73.01% viability. “MULTIPLE CHOICE” LARVAE – nine plants tested. 66.67% null; 11.11% weak; 11.11% negligible damage; and 11.11% normal in S. brasiliensis. The results indicate that the normal diet, oviposition, survival and development of P. confinis is restricted to S. brasiliensis and corroborates its potential as a biocontrol agent.

  10. Variation in manuka oil lure efficacy for capturing Xyleborus glabratus (Coleoptera: Curculionidae: Scolytinae), and cubeb oil as an alternative attractant.

    Science.gov (United States)

    Hanula, James L; Sullivan, Brian T; Wakarchuk, David

    2013-04-01

    Redbay ambrosia beetle, Xyleborus glabratus Eichoff, is an exotic species to North America vectoring a deadly vascular wilt disease of redbay [Persea borbonia (L.) Spreng], swampbay [P. palustris (Raf.) Sarg.], avocado (P. americana Mill.), and sassafras [Sassafras albidum (Nutt.) Nees]. Xyleborus glabratus is attracted to manuka oil lures, which are commercially available, and phoebe oil. Variable efficacy of manuka oil lures and insufficient availability of phoebe oil prompted us to investigate the reasons behind changes in manuka oil lure efficacy and to test cubeb oil, a readily available essential oil from Piper cubeba L. seeds, as an alternative attractant. Attraction, release rates and durations, and volatile composition of manuka oil lures manufactured in 2008 were compared with manuka oil lures manufactured in 2012, and to whole and a distilled fraction of cubeb oil. Manuka oil lures from 2008 were more attractive to X. glabratus than controls for 8 wk, whereas lures from 2012 were attractive for only 2 wk. Cubeb oil and the distilled fraction of it were as attractive as or more attractive than manuka oil in three trials. In gravimetric studies, manuka oil lures from 2008 and cubeb oil lures continued to release volatiles for 57 d, whereas lures from 2012 stopped after 16 d. The chemical composition of volatiles released from new manuka oil lures from 2008 was similar to 2012; however, a preservative (butylated hydroxytoluene) was detected in the 2008 lures. Cubeb oil was an effective attractant for X. glabratus that lasted 8-9 wk when released from bubble lures.

  11. A controlled study to determine the efficacy of Loxostylis alata (Anacardiaceae in the treatment of aspergillus in a chicken (Gallus domesticus model in comparison to ketoconazole

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    Suleiman Mohammed M

    2012-10-01

    Full Text Available Abstract Background The poultry industry due to intensive methods of farming is burdened with losses from numerous infectious agents, of which one is the fungus Aspergillus fumigatus. In a preliminary study, the extracts of Loxostylis alata A. Spreng, ex Rchb. showed good activity in vitro against A. fumigatus with a minimum inhibitory concentration of 0.07 mg/ml. For this study crude, a crude acetone extract of L. alata leaves was evaluated for its acute toxicity in a healthy chicken model and for efficacy in an infectious model of aspergillosis (A. fumigatus. Results At a dose of 300 mg/kg, the extract induced some toxicity characterised by decreased feed intake and weight loss. Consequently, 100 and 200 mg/kg were used to ascertain efficacy in the infectious model. The plant extract significantly reduced clinical disease in comparison to the control in a dose dependant manner. The extract was as effective as the positive control ketoconazole dosed at 60 mg/kg. Conclusions The results indicate that a crude extract of L. alata leaves has potential as an antifungal agent to protect poultry against avian aspergillosis.

  12. Response of Brazilian native trees to acute ozone dose.

    Science.gov (United States)

    Moura, Bárbara Baêsso; de Souza, Sílvia Ribeiro; Alves, Edenise Segala

    2014-03-01

    Ozone (O3) is a toxic secondary pollutant able to cause an intense oxidative stress that induces visual symptoms on sensitive plant species. Controlled fumigation experiment was conducted with the aim to verify the O3 sensibility of three tropical species: Piptadenia gonoachanta (Mart.) Macbr. (Fabaceae), Astronium graveolens Jacq. (Anacardiaceae), and Croton floribundus Spreng. (Euphorbiaceae). The microscopical features involved in the oxidative stress were recognized based on specific histochemical analysis. The three species showed visual symptoms, characterized as necrosis and stippling between the veins, mostly visible on the adaxial leaf surface. All the studied species presented hypersensitive-like response (HR-like), and peroxide hydrogen accumulation (H2O2) followed by cell death and proanthocyanidin oxidation in P. gonoachanta and A. graveolens. In P. gonoachanta, a decrease in chlorophyll autofluorescence occurred on symptomatic tissues, and in A. graveolens and C. floribundus, a polyphenol compound accumulation occurred. The responses of Brazilian native species were similar to those described for sensitive species from temperate climate, and microscopical markers may be useful for the detection of ozone symptoms in future studies in the field. PMID:24297466

  13. BC3EE2,9B, a synthetic carbazole derivative, upregulates autophagy and synergistically sensitizes human GBM8901 glioblastoma cells to temozolomide.

    Science.gov (United States)

    Chen, Chien-Min; Syu, Jhih-Pu; Way, Tzong-Der; Huang, Li-Jiau; Kuo, Sheng-Chu; Lin, Chung-Tien; Lin, Chih-Li

    2015-11-01

    Glioblastoma multiforme (GBM) is the most fatal form of human brain cancer. Although temozolomide (TMZ), an oral alkylating chemotherapeutic agent, improves the survival rate, the prognosis of patients with GBM remains poor. Naturally occurring carbazole alkaloids isolated from curry leaves (Murraya koenigii Spreng.) have been shown to possess a wide range of anticancer properties. However, the effects of carbazole derivatives on glioblastoma cells remain poorly understood. In the present study, anti‑glioblastoma profiles of a series of synthetic carbazole derivatives were evaluated in vitro. The most promising derivative in this series was BC3EE2,9B, which showed significant anti‑proliferative effects in GBM8401 and GBM8901 cells. BC3EE2,9B also triggered cell‑cycle arrest, most prominently at the G1 stage, and suppressed glioblastoma cell invasion and migration. Furthermore, BC3EE2,9B induced autophagy‑mediated cell death and synergistically sensitized GBM cells to TMZ cytotoxicity. The possible mechanism underlying BC3EE2,9B‑induced autophagy may involve activation of adenosine monophosphate-activated protein kinase and the attenuation of the Akt and mammalian target of the rapamycin downstream signaling pathway. Taken together, the present results provide molecular evidence for the mode of action governing the ability of BC3EE2,9B to sensitize drug‑resistant glioblastoma cells to the chemotherapeutic agent TMZ.

  14. Vasoactive and antioxidant activities of plants used in Mexican traditional medicine for the treatment of cardiovascular diseases.

    Science.gov (United States)

    Ibarra-Alvarado, C; Rojas, A; Mendoza, S; Bah, M; Gutiérrez, D M; Hernández-Sandoval, L; Martínez, M

    2010-07-01

    This study demonstrated that the aqueous extracts of plants employed in Mexican traditional medicine for the treatment of cardiovascular diseases are able to modify the tone of arterial smooth muscle. Agastache mexicana (Kunth) Lint & Epling (Labiatae), Chenopodium murale L. (Chenopodiaceae), Chirantodendron pentadactylon Larreat (Sterculiaceae), Dracocephalum moldavica L. (Labiatae), Psittacanthus calyculatus G. Don (Loranthaceae), Prunus serotina ssp. capuli (Cav. ex Spreng) McVaugh (Rosaceae), and Sechium edule Sw. (Cucurbitaceae) contain secondary metabolites that promote vascular relaxation and display antioxidant activities. As expected, their antioxidant effects showed a significant correlation with the polyphenolics content. However, a lower correlation was found between the antioxidant activity and the maximum vasodilatory effect, suggesting that the vasodilatation elicited by the plant extracts could be only partly attributed to their antioxidant properties. The extract of P. calyculatus, which displayed a maximum vasorelaxant effect that was higher than that of acetylcholine, induced endothelium-dependent vasodilatation. Futhermore, the vasorelaxant response to the P. calyculatus extract was reduced after adding an inhibitor of soluble guanylate cyclase activity, providing evidence that the NO/cGMP pathway is involved. On the other hand, the extracts of Bocconia frutescens L. (Papaveraceae), Magnolia grandiflora L. (Magnoliaceae), and Solanum rostratum Dunal (Solanaceae) induced concentration-dependent contraction of rat aortic rings, suggesting that these plants have potential health benefits for the treatment of ailments such as venous insufficiency. The pharmacological activities of the extracts studied provide scientific support for their ethnomedical use.

  15. Preferência Floral de Vespas (Hymenoptera, Vespidae no Rio Grande do Sul, Brasil

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    Alexandre Somavilla

    2012-03-01

    Abstract Wasps integrate the floral visitors’ community and they can constitute a representative portion of the pollinators. For this reason, it was aimed to know and to analyze the floral preference of the Vespidae species and to investigate the use of floral resources for these wasps. The collects were performed between 2001 and 2008 in different localities of Rio Grande do Sul state (Estrela Velha, Santa Cruz do Sul, São Francisco de Paula e Sinimbu between 08:00 at 17:00 hours, utilizing entomological nets to catch the flower-visiting wasps. The collected specimens were deposited at the Coleção Entomológica de Santa Cruz do Sul (CESC. 1.483 specimens were captured belonging to 73 wasp species, whose 78.9% were Polistinae (30 species and 21.1% Eumeninae (43 species, visiting the flowers of 33 plant species classified in 16 botanical families; the families with the larger number of plant species were Asteraceae (12, Fabaceae (4 and Apiaceae (3. The plant species with the largest number of wasps collected was Schinus terebinthifolius Raddi (616, followed by Eryngium pandanifolium L. (137 and Eryngium horridum Spreng (122. The analysis of the trophic niche overlap of 26 species with four or more visited plant species, showed an overlap equal or higher than 50% in six cases.

  16. Isolation of antibacterial compounds from Quercus dilatata L. through bioassay guided fractionation

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    Jamil Maryam

    2012-05-01

    Full Text Available Abstract Background Four medicinal plants (Chrozophora hierosolymitana Spreng, Chrysanthemum leucanthemum L., Ephedra gerardiana Wall. ex Stapf, and Quercus dilatata L. used by indigenous healers to treat various infectious diseases were selected for the present study. The major objective of the present study was isolation and characterization of antimicrobial components from the crude plant extracts using bioassay guided fractionation. Methods Seven methanolic extracts of the four plants were screened to identify any antimicrobial agents present in them. The active crude plant extract was fractionated first by solvent partitioning and then by HPLC. Characterization of the active fractions was done by using spectrophotometer. Results All the seven methanolic extracts showed low antifungal activity, however, when these extracts were tested for antibacterial activity, significant activity was exhibited by two extracts. The extract of aerial parts of Q. dilatata was most active and therefore, was selected for further analysis. Initially fractionation was done by solvent-solvent partitioning and out of six partitioned fractions, ethanol fraction was selected on the basis of results of antibacterial activity and phytochemical analysis. Further, fractionation was carried out by RP- HPLC and purified active subfractions were characterized by comparing their absorption spectra with that of the known natural products isolated from the plants of Quercus genus. Discussion and conclusion The results suggest that this is the first report of the isolated antibacterial compounds from this genus.

  17. Effect of thermal and alkaline pretreatment of giant miscanthus and Chinese fountaingrass on biogas production.

    Science.gov (United States)

    Nkemka, Valentine Nkongndem; Li, Yongqiang; Hao, Xiying

    2016-01-01

    Giant miscanthus (Miscanthus × giganteus) and Chinese fountaingrass (Pennisetum alopecuroides (L.) Spreng), cultivated for landscaping and soil conservation, are potential energy crops. The study investigated the effect of combined thermal and alkaline pretreatments on biogas production of these energy crops. The pretreatment included two types of alkali (6% CaO and 6% NaOH) at 22, 70 and 100 °C. The alkaline pretreatment resulted in a greater breakdown of the hemicellulose fraction, with CaO more effective than NaOH. Pretreatment of giant miscanthus with 6% CaO at 100 °C for 24 h produced a CH4 yield (313 mL g(-1) volatile solids (VS)) that was 1.7 times that of the untreated sample (186 mL g(-1) VS). However, pretreatment of Chinese fountaingrass with 6% CaO or 6% NaOH at 70 °C for 24 h resulted in similar CH4 yields (328 and 302 mL g(-1) VS for CaO and NaOH pretreatments) as the untreated sample (311 mL g(-1) VS). Chinese fountaingrass was more easily digestible but had a low overall CH4 yield per hectare (1,831 m(3) ha(-1) y(-1)) compared to giant miscanthus (6,868 m(3) ha(-1) y(-1)). This study demonstrates the potential of thermal/alkaline pretreatment and the use of giant miscanthus and Chinese fountaingrass for biogas production.

  18. Cytogenetics and characterization of microsatellite loci for a South American pioneer tree species, Croton floribundus.

    Science.gov (United States)

    Silvestrini, Milene; Pinto-Maglio, Cecília A F; Zucchi, Maria I; dos Santos, Flavio A M

    2013-12-01

    Despite the recent advances in plant population genetic studies, the lack of information regarding pedigree, ploidy level, or mode of inheritance for many polyploids can compromise the analysis of the molecular data produced. The aim of this study was to examine both microsatellite and cytogenetic characteristics of the pioneer tree Croton floribundus Spreng. (Euphorbiaceae) to test for the occurrence of polyploidy in the species and to evaluate its implications for the appropriate use of SSR markers. Seven microsatellite markers were developed and screened for 62 individuals from a semi-deciduous tropical forest in Brazil. Chromosome number, meiotic behavior, and pollen viability were evaluated from male flower buds. All SSR loci were highly polymorphic. The number of bivalents observed in meiosis n = 56 (2n = 8× = 112) and the maximum number of alleles per individual (Ni = 8) demonstrated the occurrence of polyploidy in C. floribundus. The normal meiotic pairing and the high pollen viability suggested that C. floribundus is a regular and stable polyploid, most likely an allopolyploid. The combined SSR and cytogenetic data provided new evidence on the origin and evolution of the species as well as assured the accurate use of SSR loci for population genetic studies of the polyploid pioneer species.

  19. Defensive Sesquiterpenoids from Leaves of Eupatorium adenophorum

    Institute of Scientific and Technical Information of China (English)

    石伟; 骆世洪; 黎胜红

    2012-01-01

    The invasive plant Eupatorium adenophorum Spreng has caused great economic loss in China, and is gravely threatening the native biodiversity and ecosystem. The plant has been phytochemically investigated for the defen- sive chemical substances in its leaves. Three active sesquiterpenoids were isolated and identified, which include a new sesquiterpenoid (1), and two known sesquiterpenoids (2, 3). Their structures were established by spectroscopic studies such as 1D- and 2D-NMR and MS analyses. Meanwhile, the antifeedant activities of these compounds against two generalist plant-feeding insects, Helicoverpa armigera and Spodoptera exigua, were carried out. Com- pound 1 showed significant antifeedant activity against S. exigua with ECs0 = 7.46 μg/cm^2, while compounds 2 and 3 were more active against H. armigera (EC50=2.57 and 3.04 μg/cm^2 respectively). These findings suggest a de- fensive role of sesquiterpenoids in E. adenophorum against herbivores.

  20. Effects of the Ethyl Acetate Fraction of Alchornea triplinervia on Healing Gastric Ulcer in Rats.

    Science.gov (United States)

    Lima, Zeila P; Bonamin, Flavia; Calvo, Tamara R; Vilegas, Wagner; Santos, Lourdes C; Rozza, Ariane L; Pellizzon, Claudia H; Rocha, Lucia R M; Hiruma-Lima, Clélia A

    2011-01-01

    Alchornea triplinervia (Spreng.) Muell. Arg (Euphorbiaceae) is a medicinal plant commonly used by people living in the Cerrado region of Brazil to treat gastrointestinal ulcers. We previously described the gastroprotective action of methanolic extract (ME) of Alchornea triplinervia and the ethyl acetate fraction (EAF) in increasing of prostaglandin E₂ (PGE₂) gastric levels in the mucosa. In this work we evaluated the effect of EAF in promoting the healing process in rats with acetic acid-induced gastric ulcers. In addition, toxicity was investigated during treatment with EAF. After 14 days of treatment with EAF, the potent stimulator of gastric cell proliferation contributed to the acceleration of gastric ulcer healing. Upon immunohistochemical analysis, we observed a pronounced expression of COX-2, mainly in the submucosal layer. The 14-day EAF treatment also significantly increased the number of neutrophils in the gastric mucosa regeneration area. The EAF induced angiogenesis on gastric mucosa, observed as an increase of the number of blood vessels supplying the stomach in rats treated with EAF. Oral administration for 14 days of the ethyl acetate fraction from Alchornea triplinervia accelerated the healing of gastric ulcers in rats by promoting epithelial cell proliferation, increasing the number of neutrophils and stimulation of mucus production. This fraction, which contained mainly phenolic compounds, contributed to gastric mucosa healing.

  1. Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

    Directory of Open Access Journals (Sweden)

    Aline Cavalcanti De Queiroz

    2014-01-01

    Full Text Available This study investigates the leishmanicidal activity of five species of plants used in folk medicine in endemic areas of the state of Alagoas, Brazil. Data were collected in the cities of Colonia Leopoldina, Novo Lino, and União dos Palmares, Alagoas state, from patients with cutaneous leishmaniasis (Leishmania amazonensis who use medicinal plants to treat this disease. Plants extracts were tested at a concentration of 1–100 μg/mL in all experiments, except in an assay to evaluate activity against amastigotes, when 10 μg/mL was used. All plants extracts did not show deleterious activity to the host cell evidenced by LDH assay at 100, 10, and 1 μg/mL after 48 h of incubation. The plants extracts Hyptis pectinata (L. Poit, Aloe vera L., Ruta graveolens L., Pfaffia glomerata (Spreng. Pedersen, and Chenopodium ambrosioides L. exhibited direct activity against extracellular forms at 100 μg/mL; these extracts inhibited growth by 81.9%, 82.9%, 74.4%, 88.7%, and 87.4%, respectively, when compared with promastigotes. The plants extracts H. pectinata, A. vera, and R. graveolens also significantly diminished the number of amastigotes at 10 μg/mL, inhibiting growth by 85.0%, 40.4%, 94.2%, and 97.4%, respectively, when compared with control. Based on these data, we conclude that the five plants exhibited considerable leishmanicidal activity.

  2. EVALUATION OF NEUROPHARMACOLOGICAL ACTIVITIES OF STEPHANIA VENOSA HERB CONSUMPTION IN HEALTHY RATS

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    Terdthai Tong-Un

    2012-01-01

    Full Text Available Public concern on mental health has noticeably increased given the high prevalence of neuropsychiatric disorders especially anxiety and depression. Most of the drugs for these conditions used nowadays have adverse side effects so the need for newer, better-tolerated and more efficacious treatments is remaining high. Growing attention is being paid to traditional herbal medicines. Stephania venosa Spreng (S. venosa; SV is a Thai traditional herb which has been used for cancer treatment as well as an aphrodisiac. In addition, accumulating lines of evidence reported that the ethanol extract of SV exhibited an antioxidant and exerted acetylcholinesterase inhibitory. However, the in vivo neurophamacological activities of this plant have never been studied. The aim of this study was to examine the neurophamacological effects of a crude extract of SV introduced orally at various doses ranging from 5, 10 and 20 mg kg-1 BW once daily for a period of 2 weeks. The anxiolytic and anti-depression like activities were determined after single administration, 1 and 2 weeks of treatment using elevated plus maze and forced swimming test respectively. Only SV 20 mg kg-1 treated rats exhibited a significant anxiolytic effect at all treatment duration. Unfortunately, this substance failed to show anti-depression like activity. Our findings provide a potential of the SV consumption might be used as a novel therapeutic strategy for the anti-anxiety disorder. However, further investigations about possible active ingredients and the precise underlying mechanisms are still necessary.

  3. New high-performance liquid chromatography-dad method for analytical determination of arbutin and hydroquinone in rat plasma

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    F R Gallo

    2015-01-01

    Full Text Available Natural substances present in herbal preparations should be carefully used because they can give toxic or therapeutic effects despite of their amount or the way of administration. The safety of products of vegetable origin must be assessed before commercialisation by monitoring the active ingredients and their metabolites. This study was therefore designed to identify and quantify arbutin and its metabolite hydroquinone, naturally present in Arctostaphylos uva-ursi (L. Spreng plant in rat plasma, after an acute and subacute administration of aqueous arbutin solution in Wistar rats. For this purpose a reversed-phase high-performance liquid chromatography coupled with photodiode array detection was developed to assess the pharmacokinetic of arbutin and hydroquinone in plasma of female rats treated with aqueous arbutin solutions. The detection (arbutin: 0.0617 µg/ml and hydroquinone 0.0120 µg/ml and quantification (arbutin: 0.2060 µg/ml and hydroquinone: 0.0400 µg/ml limits were determined. At the arbutin concentration level of 10.7 µg/ml repeatability was 13.33% and its recovery 93.4±6.93%, while at the hydroquinone concentration level of 10.6 µg/ml repeatability was 11.66% and its recovery 92.9±7.75%. Furthermore the method was fully validated and the obtained data indicate that the new method provides good performances.

  4. New High-performance Liquid Chromatography-DAD Method for Analytical Determination of Arbutin and Hydroquinone in Rat Plasma.

    Science.gov (United States)

    Gallo, F R; Pagliuca, G; Multari, G; Panzini, G; D'amore, E; Altieri, I

    2015-01-01

    Natural substances present in herbal preparations should be carefully used because they can give toxic or therapeutic effects despite of their amount or the way of administration. The safety of products of vegetable origin must be assessed before commercialisation by monitoring the active ingredients and their metabolites. This study was therefore designed to identify and quantify arbutin and its metabolite hydroquinone, naturally present in Arctostaphylos uva-ursi (L.) Spreng plant in rat plasma, after an acute and subacute administration of aqueous arbutin solution in Wistar rats. For this purpose a reversed-phase high-performance liquid chromatography coupled with photodiode array detection was developed to assess the pharmacokinetic of arbutin and hydroquinone in plasma of female rats treated with aqueous arbutin solutions. The detection (arbutin: 0.0617 µg/ml and hydroquinone 0.0120 µg/ml) and quantification (arbutin: 0.2060 µg/ml and hydroquinone: 0.0400 µg/ml) limits were determined. At the arbutin concentration level of 10.7 µg/ml repeatability was 13.33% and its recovery 93.4±6.93%, while at the hydroquinone concentration level of 10.6 µg/ml repeatability was 11.66% and its recovery 92.9±7.75%. Furthermore the method was fully validated and the obtained data indicate that the new method provides good performances.

  5. Effect of Hg, As and Pb on biomass production, photosynthetic rate, nutrients uptake and phytochelatin induction in Pfaffia glomerata.

    Science.gov (United States)

    Gupta, D K; Huang, H G; Nicoloso, F T; Schetinger, M R; Farias, J G; Li, T Q; Razafindrabe, B H N; Aryal, N; Inouhe, M

    2013-11-01

    Plantlets of Pfaffia glomerata (Spreng.) were exposed for 28 days to three different metal/metalloid (Hg, Pb and As) with different levels (Hg 1; As 25, 50, 100 and Pb 100 and 400 μM) to analyze the possible phytochelatin initiation and affects on growth and photosynthetic pigments vis-à-vis metal accumulation potential of plants. The plantlets showed significant Hg, As and Pb accumulation in roots (150, 1267.67 and 2129 μg g(-1) DW respectively); however, a low root to shoot metal translocation was observed. It was interesting to note that all tested macronutrient (Mg, K, Ca) was higher in shoots and just opposite in case of micronutrients (Cu, Fe, Zn), was recorded highest in roots. The growth of plantlets (analyzed in terms of length and dry weight) was negatively affected by various metal treatments. In addition, the level of photosynthetic pigments alters significantly in response to all metal/metalloid treatment. In response to all tested metal/metalloids in plants only As induced phytochelatins (PC2, PC3 and PC4) in roots, and in shoots, GSH was observed in all tested metal/metalloids. In conclusion, P. glomerata plantlets could not cooperatively induce phytochelatins under any of Hg and Pb levels.

  6. Acaricidal activity of Annonaceae fractions against Tetranychus tumidus and Tetranychus urticae (Acari: Tetranychidae and the metabolite profile of Duguetia lanceolata (Annonaceae using GC-MS

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    Dejane Santos Alves

    2015-12-01

    Full Text Available Species of the Tetranychus genus feed on plant tissues, which reduces the rate of photosynthesis and can lead to the death of plant tissues. As a result, considerable production losses are caused by these arthropods. Thus, in order to aid in the development of new products for the control of Tetranychus tumidus Banks and Tetranychus urticae Koch (Acari: Tetranychidae, the initial objective of this study was to select Annonaceae derived fractions that were soluble in dichloromethane and have acaricidal activity. Then, an exploratory analysis of the metabolite profile of the most successful fraction was performed by gas chromatography-mass spectrometry (GC-MS. Among the dichloromethane soluble fractions derived from Annona cacans Warm., Annona coriacea Mart., Annona neolaurifolia H. Rainer, Annona sylvatica A.St.-Hil., Duguetia lanceolata A.St.-Hil., Guatteria australis A.St.-Hil., Xylopia brasiliensis Spreng., Xylopia emarginata Mart. and Xylopia sericea A.St.-Hil., only the fraction from D. lanceolata stem bark reduced the survival of T. tumidus females. However, ovicidal activity was not detected when D. lanceolata stem bark was evaluated against T. tumidus eggs. Further, we studied the effect of dichloromethane soluble fractions from D. lanceolata leaves, berry fruits and stem bark on T. urticae, and the stem bark was found to be the most active fraction against T. urticae. The metabolite profile analysis of D. lanceolata stem bark by GC-MS, suggested that the main constituents were 2,4,5-trimethoxystyrene and trans-asarone.

  7. A win-win technique of stabilizing sand dune and purifying paper mill black-liquor

    Institute of Scientific and Technical Information of China (English)

    WANG Hanjie; Penning de Vries FRITS; JIN Yongcan

    2009-01-01

    The principle and technique were reported here to produce lignin-based sand stabilizing material (LSSM) using extracted lignin from black liquor of straw paper mills. Field tests by using LSSM to stabilize and green sand dunes started in 2002. The field experiment was carried out in August 2005 when the newly formed plant community was 3 years old. The results from the comprehensive field experiment demonstrated that unlike polyvinyl acetate or foamed asphalt commonly used for dune stabilization, LSSM was plant-friendly material and could be used in combination with seeding and planting of desert species. By the help of LSSM, the desert species (i.e., Agriophyllum squarrosum (L.) Moq. and Artemisia desertorum Spreng. etc.) could be used to form community in 2-3 yeas and to stabilize sand dune effectively. The newly formed community was sustainable under an extremely dry climate conditions. The organic matter and total nitrogen in the soil increased significantly as the community were formed, while the change in P and K contents of the soil was negligible.

  8. REGISTRO DA COCHONILHA-ROSADA-DO-HIBISCO INFESTANDO FRUTÍFERAS EM MACEIÓ, ALAGOAS, BRASIL

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    SÔNIA MARIA FORTI BROGLIO

    2015-01-01

    Full Text Available This study reported for the first time the occurrence of the pink hibiscus mealybug Maconelli-coccus hirsutus (Green, 1908 (Hemiptera: Pseudococcidae in Maceió, Alagoas, Brazil attacking the following fruit species: soursop (Annona muricata L. (Annonaceae, guava (Psidium guajava L. (Myrtaceae, mango (Mangifera indica L. (Anacardiaceae, carambola (Averrhoa carambola L. (Oxalidaceae, acerola (Malpighia glaba L. (Malpighiaceae, cajazeiro (Spondias lutea L. (Anacardiaceae and cupuaçuzeiro (Theobroma gran-diflorum (Willd. ex Spreng. Schum. (Malvaceae. The percentages of injured plants were recorded for each plant species in the field. Damaged plants showed short internodes, wrinkled leaves, fruit deformation, and sooty mold developed on the honeydew excreted by the mealybugs. Infested plant structures were taken to the Laboratory of Entomology of the Center for Agricultural Sciences, of the Federal University of Alagoas, where the mealybug species was identified through morphological characteristics. The following injury percentages were recorded on the plants: soursop (100%, guava (10%, mango (10%, carambola (100%, acerola (100%, cajá (50% and cupuaçu (100%. The mealybug collected in Maceió, AL; Brazil was identified as Maconelli-coccus hirsutus (Green, 1908 (Hemiptera: Pseudococcidae. It was the first record of this species in the state of Alagoas and infestation by pink hibiscus mealybug on the host plants mango, acerola and cajazeiro in Brazil.

  9. Antileishmanial Activity of Medicinal Plants Used in Endemic Areas in Northeastern Brazil

    Science.gov (United States)

    De Queiroz, Aline Cavalcanti; Dias, Thays de Lima Matos Freire; Da Matta, Carolina Barbosa Brito; Cavalcante Silva, Luiz Henrique Agra; de Araújo-Júnior, João Xavier; de Araújo, Givanildo Bernardino; Moura, Flávia de Barros Prado; Alexandre-Moreira, Magna Suzana

    2014-01-01

    This study investigates the leishmanicidal activity of five species of plants used in folk medicine in endemic areas of the state of Alagoas, Brazil. Data were collected in the cities of Colonia Leopoldina, Novo Lino, and União dos Palmares, Alagoas state, from patients with cutaneous leishmaniasis (Leishmania amazonensis) who use medicinal plants to treat this disease. Plants extracts were tested at a concentration of 1–100 μg/mL in all experiments, except in an assay to evaluate activity against amastigotes, when 10 μg/mL was used. All plants extracts did not show deleterious activity to the host cell evidenced by LDH assay at 100, 10, and 1 μg/mL after 48 h of incubation. The plants extracts Hyptis pectinata (L.) Poit, Aloe vera L., Ruta graveolens L., Pfaffia glomerata (Spreng.) Pedersen, and Chenopodium ambrosioides L. exhibited direct activity against extracellular forms at 100 μg/mL; these extracts inhibited growth by 81.9%, 82.9%, 74.4%, 88.7%, and 87.4%, respectively, when compared with promastigotes. The plants extracts H. pectinata, A. vera, and R. graveolens also significantly diminished the number of amastigotes at 10 μg/mL, inhibiting growth by 85.0%, 40.4%, 94.2%, and 97.4%, respectively, when compared with control. Based on these data, we conclude that the five plants exhibited considerable leishmanicidal activity. PMID:25126099

  10. Biology and systematics of the New World Phyllocnistis Zeller leafminers of the avocado genus Persea (Lepidoptera, Gracillariidae

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    Donald Davis

    2011-05-01

    Full Text Available Four New World species of Phyllocnistis Zeller are described from serpentine mines in Persea (Family Lauraceae. Phyllocnistis hyperpersea, new species, mines the upper leaf surfaces of avocado, Persea americana Mill., and red bay, Persea borbonia (L. Spreng. and ranges over much of the southeastern United States into Central America. Phyllocnistis subpersea, new species, mines the underside and occasionally upper sides of new leaves of Persea borbonia in southeastern United States. Phyllocnistis longipalpa, new species, known only from southern Florida also mines the undersides of new leaves of Persea borbonia. Phyllocnistis perseafolia, new species, mines both leaf surfaces and possibly fruits of Persea americana in Colombia, South America. As in all known species of Phyllocnistis, the early instars are subepidermal sapfeeders in young (not fully hardened foliage, and the final instar is an extremely specialized, nonfeeding larval form, whose primary function is to spin the silken cocoon, at the mine terminus, prior to pupation. Early stages are illustrated and described for three of the species. The unusual morphology of the pupae, particularly the frontal process of the head, is shown to be one of the most useful morphological sources of diagnostic characters for species identification of Phyllocnistis. COI barcode sequence distances are provided for the four proposed species and a fifth, undescribed species from Costa Rica.

  11. Relationships between structure of the tree component and environmental variables in a subtropical seasonal forest in the upper Uruguay River valley, Brazil

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    Máida Ariane de Mélo

    2013-12-01

    Full Text Available This study aimed to analyze relationships among the structure of the tree component, edaphic variables and canopy discontinuity along a toposequence in a seasonal upland (hillside forest in southern Brazil. Soil and vegetation were sampled in 25 plots of 20 × 20 m each. We described the vegetation in terms of structure, richness and diversity, as well as by species distribution patterns. We evaluated canopy continuity, determined sloping and calculated spatial coordinates. We applied partial canonical correspondence analysis (pCCA to determine whether species distribution correlated with environmental and spatial variables. We identified 1201 individuals belonging to 76 species within 30 families. The species with highest density and frequency were Gymnanthes concolor Spreng., Calyptranthes tricona D.Legrand, Eugenia moraviana O.Berg and Trichilia claussenii DC. The pCCAs indicated significant correlations with environmental and spatial variables. Sand content, boron content and soil density collectively explained 36.17% of the species matrix variation (total inertia, whereas the spatial variables x, y and xy² collectively explained 14.27%. The interaction between environmental and spatial variables explained nearly 4.5%. However, 45.05% remained unexplained, attributed to stochastic variation or unmeasured variables. Terrain morphology and canopy discontinuity had no apparent influence on richness, and changes in species distribution were correlated with sloping, which affects soil features and determines the directional distribution of some species.

  12. Acetylcholinesterase inhibitory activity of Thai traditional nootropic remedy and its herbal ingredients.

    Science.gov (United States)

    Tappayuthpijarn, Pimolvan; Itharat, Arunporn; Makchuchit, Sunita

    2011-12-01

    The incidence of Alzheimer disease (AD) is increasing every year in accordance with the increasing of elderly population and could pose significant health problems in the future. The use of medicinal plants as an alternative prevention or even for a possible treatment of the AD is, therefore, becoming an interesting research issue. Acetylcholinesterase (AChE) inhibitors are well-known drugs commonly used in the treatment of AD. The aim of the present study was to screen for AChE inhibitory activity of the Thai traditional nootropic recipe and its herbal ingredients. The results showed that ethanolic extracts of four out of twenty-five herbs i.e. Stephania pierrei Diels. Kaempfera parviflora Wall. ex Baker, Stephania venosa (Blume) Spreng, Piper nigrum L at 0.1 mg/mL showed % AChE inhibition of 89, 64, 59, 50; the IC50 were 6, 21, 29, 30 microg/mL respectively. The other herbs as well as combination of the whole recipe had no synergistic inhibitory effect on AChE activity. However some plants revealed antioxidant activity. More research should have be performed on this local wisdom remedy to verify the uses in scientific term. PMID:22619927

  13. Effects of the Ethyl Acetate Fraction of Alchornea triplinervia on Healing Gastric Ulcer in Rats

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    Clélia A. Hiruma-Lima

    2011-10-01

    Full Text Available Alchornea triplinervia (Spreng. Muell. Arg (Euphorbiaceae is a medicinal plant commonly used by people living in the Cerrado region of Brazil to treat gastrointestinal ulcers. We previously described the gastroprotective action of methanolic extract (ME of Alchornea triplinervia and the ethyl acetate fraction (EAF in increasing of prostaglandin E2 (PGE2 gastric levels in the mucosa. In this work we evaluated the effect of EAF in promoting the healing process in rats with acetic acid-induced gastric ulcers. In addition, toxicity was investigated during treatment with EAF. After 14 days of treatment with EAF, the potent stimulator of gastric cell proliferation contributed to the acceleration of gastric ulcer healing. Upon immunohistochemical analysis, we observed a pronounced expression of COX-2, mainly in the submucosal layer. The 14-day EAF treatment also significantly increased the number of neutrophils in the gastric mucosa regeneration area. The EAF induced angiogenesis on gastric mucosa, observed as an increase of the number of blood vessels supplying the stomach in rats treated with EAF. Oral administration for 14 days of the ethyl acetate fraction from Alchornea triplinervia accelerated the healing of gastric ulcers in rats by promoting epithelial cell proliferation, increasing the number of neutrophils and stimulation of mucus production. This fraction, which contained mainly phenolic compounds, contributed to gastric mucosa healing.

  14. Antioxidant and anti-glycation activities correlates with phenolic composition of tropical medicinal herbs

    Institute of Scientific and Technical Information of China (English)

    JS Ramkissoon; MF Mahomoodally; N Ahmed; AH Subratty

    2013-01-01

    Objective: To determine the contribution of total phenolic content (TPC) in glycation inhibitory activity of common tropical medicinal food and spices with potential antioxidative properties. Methods: In vitro glucose-bovine serum albumin (BSA) assay was used. Ethanolic extracts of ten common household condiments/herbs (Allium sativum, Zingiber officinale, Thymus vulgaris, Petroselinum crispum, Murraya koenigii Spreng, Mentha piperita L., Curcuma longa L., Allium cepa L., Allium fistulosum and Coriandrum sativum L.) were evaluated for antioxidative activity by 2,2-diphenyl-2-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP) and the TPC, flavonoid and tannins content were determined. Results: Findings showed good correlation between TPC/DPPH (r= 0.8), TPC/FRAP (r= 0.8), TPC/Anti-glycation (r=0.9), DPPH/Anti-glycation (r= 0.6), FRAP/Anti-glycation (r = 0.9), Flavonoid/Anti-glycation (r= 0.7) and Tannins/Anti-glycation (r = 0.8) and relatively fair correlation for TPC/Flavonoids (r = 0.5) and TPC/Tannins (r =0.5). Results imply that these plants are potential sources of natural antioxidants which have free radical scavenging activity and might be used for reducing oxidative stress. Conclusions: The positive glycation inhibitory and antioxidative activities of these tropical herbs suggest a possible role in targeting ageing, diabetic complications and oxidative stress related diseases.

  15. USE OF ULTRASOUND FOR ESTIMATIVE OF MECHANICAL PROPERTIES OF Peltophorum dubium WOOD

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    Diego Martins Stangerlin

    2010-11-01

    Full Text Available The aimed this study was evaluate the use of dynamic elastic constant obtained by non-destructive test as a parameter in the estimative of mechanical properties of Peltophorum dubium (Spreng. Taub. wood obtained by destructive test. For this, ultrasonic equipment (not-destructive method was utilized with point-contact transducersof 45 kHz. The ultrasonic velocity was determined considering the wave transmission along the length of the samples, which dimensions of 2.5 x 2.5 x 41 cm in thickness, width and length, respectively. Based on the relationship between ultrasonic velocity and densityof the wood was determined the dynamic elastic constant. The ultrasonic method sensibility was evaluated with subsequent static-bending test where modulus of elasticity and rupture determination. The results showed that ultrasonic method it’s fast and moderately efficient tool for inference non-destructive of mechanical properties. However, was verified difference in the adjustment of statistical models generated to estimate the properties, and found the best regression parameters to estimate the modulus of elasticity,compared with the modulus of rupture.

  16. Effect of chipping on emergence of the redbay ambrosia beetle (Coleoptera: Curculionidae: Scolytinae) and recovery of the laurel wilt pathogen from infested wood chips.

    Science.gov (United States)

    Spence, D J; Smith, J A; Ploetz, R; Hulcr, J; Stelinski, L L

    2013-10-01

    Significant mortality ofredbay trees (Persea borbonia (L.) Spreng.) in the southeastern United States has been caused by Raffaelea lauricola, T.C. Harr., Fraedrich, & Aghayeva (Harrington et al. 2008), a fungal symbiont of the exotic redbay ambrosia beetle, Xyleborus glabratus, Eichhoff (Fraedrich et al. 2008). This pathogen causes laurel wilt, which is an irreversible disease that can kill mature trees within a few weeks in summer. R. lauricola has been shown to be lethal to most native species of Lauraceae and cultivated avocado (Persea americana Mill.) in the southeastern United States. In this study, we examined the survival of X. glabratus and R. lauricola in wood chips made from infested trees by using a standard tree chipper over a 10-wk period. After 2 wk, 14 X. glabratus were recovered from wood chips, whereas 339 X. glabratus emerged from nonchipped bolts. R. lauricola was not found 2 d postchipping from wood chips, indicating that the pathogen is not likely to survive for long inside wood chips. In contrast, R. lauricola persisted in dead, standing redbay trees for 14 mo. With large volumes of wood, the potential for infested logs to be moved between states or across U.S. borders is significant. Results demonstrated that chipping wood from laurel wilt-killed trees can significantly reduce the number of X. glabratus and limit the persistence of R. lauricola, which is important for sanitation strategies aimed at limiting the spread of this disease. PMID:24224251

  17. Selecting matched root architecture in tree pairs to be used for assessing N 2 fixation based on soil- 15N-labelling

    Science.gov (United States)

    Nasr, Hafedh; Ghorbel, Mohamed Habib; Wallander, Håkan; Dommergues, Yvon René

    2005-03-01

    It is commonly assumed that soil- 15N-labelling provides reliable estimates of N 2 fixation in trees by matching N 2-fixing and non-N 2-fixing tree pairs. As root system is a key parameter in determining suitability of the tree pairs, we compared root architecture of Acacia cyanophylla Lindl. and Casuarina glauca Sieber ex. Spreng. (two N 2-fixing trees) with Eucalyptus camaldulensis Dehn. and Ceratonia siliqua L. (two non-N 2-fixing trees) at 4-year-old in Mediterranean-semiarid zone. The rhizobium strain used appeared more motile than Frankia strain. A. cyanophylla and E. camaldulensis had extensive rooting area and volume of fine roots, and both species tended to develop marked horizontal rooting, compared to C. glauca and C. siliqua. Characteristics of fine- and horizontal-root components can be used in selecting matched root systems of N 2-fixing and reference-paired trees. Root architecture of C. glauca was more similar to C. siliqua, than to E. camaldulensis, and that of A. cyanophylla was more similar to E. camaldulensis than to C. siliqua. Accordingly, E. camaldulensis is an appropriate reference to estimate actual N 2 fixation by A. cyanophylla, and C. siliqua is an appropriate reference for C. glauca, when using soil- 15N-labelling method in the prevailing site environment.

  18. New High-performance Liquid Chromatography-DAD Method for Analytical Determination of Arbutin and Hydroquinone in Rat Plasma.

    Science.gov (United States)

    Gallo, F R; Pagliuca, G; Multari, G; Panzini, G; D'amore, E; Altieri, I

    2015-01-01

    Natural substances present in herbal preparations should be carefully used because they can give toxic or therapeutic effects despite of their amount or the way of administration. The safety of products of vegetable origin must be assessed before commercialisation by monitoring the active ingredients and their metabolites. This study was therefore designed to identify and quantify arbutin and its metabolite hydroquinone, naturally present in Arctostaphylos uva-ursi (L.) Spreng plant in rat plasma, after an acute and subacute administration of aqueous arbutin solution in Wistar rats. For this purpose a reversed-phase high-performance liquid chromatography coupled with photodiode array detection was developed to assess the pharmacokinetic of arbutin and hydroquinone in plasma of female rats treated with aqueous arbutin solutions. The detection (arbutin: 0.0617 µg/ml and hydroquinone 0.0120 µg/ml) and quantification (arbutin: 0.2060 µg/ml and hydroquinone: 0.0400 µg/ml) limits were determined. At the arbutin concentration level of 10.7 µg/ml repeatability was 13.33% and its recovery 93.4±6.93%, while at the hydroquinone concentration level of 10.6 µg/ml repeatability was 11.66% and its recovery 92.9±7.75%. Furthermore the method was fully validated and the obtained data indicate that the new method provides good performances. PMID:26798166

  19. Ethnomedicine and ethnobotany of fright, a Caribbean culture-bound psychiatric syndrome

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    Quinlan Marsha B

    2010-02-01

    Full Text Available Abstract Background "Fright" is an English-speaking Caribbean idiom for an illness, or ethnomedical syndrome, of persistent distress. A parallel ethnopsychiatric idiom exists in the French Antilles as sésisma. Fright is distinct from susto among Hispanics, though both develop in the wake of traumatic events. West Indian ethnophysiology (ethnoanatomy theorizes that an overload of stressful emotions (fear, panic, anguish or worry causes a cold humoral state in which blood coagulates causing prolonged distress and increased risks of other humorally cold illnesses. Methods Qualitative data on local explanatory models and treatment of fright were collected using participant-observation, informal key informant interviews and a village health survey. Ethnobotanical and epidemiological data come from freelist (or "free-list" tasks, analyzed for salience, with nearly all adults (N = 112 of an eastern village in Dominica, and a village survey on medicinal plant recognition and use (N = 106. Results Along with prayer and exercise, three herbs are salient fright treatments: Gossypium barbadense L., Lippia micromera Schauer, and, Plectranthus [Coleus] amboinicus [Loureiro] Sprengel. The survey indicated that 27% of village adults had medicated themselves for fright. Logistic regression of fright suffering onto demographic variables of age, education, gender, parental status and wealth measured in consumer goods found age to be the only significant predictor of having had fright. The probability of having (and medicating for fright thus increases with every year. Conclusions While sufferers are often uncomfortable recalling personal fright experiences, reporting use of medicinal plants is less problematic. Inquiry on fright medical ethnobotany (or phytotherapies serves as a proxy measurement for fright occurrence. Cross-cultural and ethnopharmacology literature on the medicinal plants suggests probable efficacy in accord with Dominican ethnomedical notions

  20. Toxicity of essential oil from Indian borage on the larvae of the African malaria vector mosquito, Anopheles gambiae

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    Kweka Eliningaya J

    2012-12-01

    Full Text Available Abstract Background Essential oils are currently studied for the control of different disease vectors, because of their efficacy on targeted organisms. In the present investigation, the larvicidal potential of essential oil extracted from Indian borage (Plectranthus amboinicus was studied against the African anthropophagic malaria vector mosquito, Anopheles gambiae. The larvae of An. gambiae s.s laboratory colony and An. gambiae s.l of wild populations were assayed and the larval mortality was observed at 12, 24 and 48 h after exposure period with the concentrations of 3.125, 6.25, 12.5, 25, 50 and 100 ppm. Findings Larval mortality rates of the essential oil was entirely time and dose dependent. The LC50 values of the laboratory colony were 98.56 (after 12h 55.20 (after 24 h and 32.41 ppm (after 48 h and the LC90 values were 147.40 (after 12h, 99.09 (after 24 h and 98.84 ppm (after 48 h. The LC50 and LC90 values of the wild population were 119.52, 179.85 (after 12h 67.53, 107.60 (after 24 h and 25.51, 111.17 ppm (after 48 h respectively. The oil showed good larvicidal potential after 48 h of exposure period against An. gambiae. The essential oil of Indian borage is a renowned natural source of larvicides for the control of the African malaria vector mosquito, An. gambiae. Conclusion The larvicidal efficacy shown by plant extracts against An. gambiae should be tested in semi field and small scale trials for effective compounds to supplement the existing larval control tools.