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Sample records for ambient ph-regulated enzime

  1. Ambient pH-regulated enzime secretion in endophytic and pathogenic isolates of the fungal genus Colletotrichum Secreção de enzimas mediada pelo pH do ambiente em isolados patogênicos e endofíticos do fungo Colletotrichum

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    Walter Maccheroni Jr.

    2004-06-01

    ênicos pertencentes a diferentes espécies de Colletotrichum. Para todas as enzimas e em todos os isolados, observou-se um padrão de secreção dependente dos valores do pH do ambiente. Isolados endofíticos e patogênicos apresentaram padrões distintos de secreção de protease, com ótimos em pH de crescimento alcalino e ácido, respectivamente. Em meio líquido, uma fosfatase ácida Pi-repressível, secretada por um isolado endofítico, respondeu ao pH do ambiente, apresentando um aumento de 14 vezes na sua atividade específica durante o crescimento do fungo em meio ácido, quando comparado a meio alcalino. Além disso, foi clonada parte do gene pacC de Colletotrichum, o qual codifica um fator de transcrição responsável pela regulação dependente do pH do ambiente. É plausível a hipótese de que o pH ambiente é um fator de amplo espectro controlando a secreção enzimática durante as interações fungo-hospedeiro por meio de um circuito genético conservado.

  2. Complexo enzimático para suínos: digestão, metabolismo, desempenho e impacto ambiental Enzyme complex for swine: nutrient digestion, metabolism, performance and environmental impact

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    Urbano dos Santos Ruiz

    2008-03-01

    Full Text Available Foram conduzidos dois experimentos para avaliar a suplementação enzimática (amilase, pentosanase, celulase, protease e a-galactosidase em rações à base de milho e farelo de soja para suínos. No Exp 1, foram determinadas as digestibilidades aparentes da energia, matéria seca, proteína e das fibras das rações, com ou sem o complexo enzimático, para suínos machos castrados, com 19,96 ± 2,90 kg de peso vivo. Foi utilizado o método da coleta total de fezes e as rações foram formuladas com níveis reduzidos de energia, proteína e aminoácidos. No Exp 2, foram mensurados o desempenho, a excreção de sólidos totais e voláteis, matéria mineral, nitrogênio, macro e microminerais nas fezes, em relação ao desempenho, de suínos machos castrados dos 50 aos 151 dias de idade, com peso médio inicial de 18,34 ± 1,35 kg. Foram utilizadas três dietas, sendo uma ração formulada para atender ou exceder as exigências nutricionais dos animais, de acordo com o NRC (1998, e outras duas com níveis reduzidos de energia, proteína e aminoácidos, suplementadas ou não com o complexo enzimático. Foi utilizado o delineamento em blocos casualizados. A suplementação enzimática em rações contendo milho e farelo de soja não promoveu incrementos na digestibilidade de nutrientes, não melhorou o desempenho dos animais e também não reduziu a excreção de resíduos pelas fezes. Mais estudos são necessários para testar novas matrizes e diferentes níveis do complexo enzimático.Two experiments were conducted to evaluate dietary enzyme supplementation (amylase, pentosanase, celulase and a-galactosidase in corn-soy diets for swine. In the Exp 1, the apparent digestibilities of energy, dry matter, protein and fibers of the feeds were determined, using a nutritional matrix with or without enzyme supplementation for barrows with 19.96 ± 2.90 kg body weight. The total feces collection method was used and the diets were formulated with reduced

  3. Involvement of Histidine Residue His382 in pH Regulation of MCT4 Activity.

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    Shotaro Sasaki

    Full Text Available Monocarboxylate transporter 4 (MCT4 is a pH-dependent bi-directional lactate transporter. Transport of lactate via MCT4 is increased by extracellular acidification. We investigated the critical histidine residue involved in pH regulation of MCT4 function. Transport of lactate via MCT4 was measured by using a Xenopus laevis oocyte expression system. MCT4-mediated lactate transport was inhibited by Zn2+ in a pH physiological condition but not in an acidic condition. The histidine modifier DEPC (diethyl pyrocarbonate reduced MCT4 activity but did not completely inactivate MCT4. After treatment with DEPC, pH regulation of MCT4 function was completely knocked out. Inhibitory effects of DEPC were reversed by hydroxylamine and suppressed in the presence of excess lactate and Zn2+. Therefore, we performed an experiment in which the extracellular histidine residue was replaced with alanine. Consequently, the pH regulation of MCT4-H382A function was also knocked out. Our findings demonstrate that the histidine residue His382 in the extracellular loop of the transporter is essential for pH regulation of MCT4-mediated substrate transport activity.

  4. KARAKTERISASI ENZIM KITOSANASE DAR] ISOLAT BAKTERI KPU 2123 DAN APLIKASINYA UNTUK PRODUKS1 OLIGOMER KITOSAN

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    Yusro Nuri Fawzya

    2009-06-01

    Full Text Available Penelitian ini merupakan sebagian dari rangkaian penelitian mengenai eksplorasi enzim kitinolitik dari mikroba lingkungan laut, khususnya dari limbah udang. Tujuan penelitian ini adalah untuk mengidentifikasi isolat bakteri KPU 2123 dari limbah udang, mengkarakterisasi dan mengaplikasikan enzim kitosanase yang dihasilkan oleh bakteri tersebut untuk produksi oligomer kitosan dan menguji bioaktivitas oligomer kitosan tersebut sebagai antitumor dan antibakteri. Karakterisasi enzim dilakukan dengan menguji aktivitas enzim pada berbagai suhu dan pH. Selain itu juga ditentukan besarnya aktivitas yang tersisa setelah enzim diinkubasi pada suhu dan lama waktu tertentu. Pengaruh ion logam terhadap aktivitas enzim juga dilihat dengan mereaksikan enzim dengan 1 mM ion logam dalam bentuk larutan khlorida. Hasil penelitian menunjukkan bahwa berdasarkan analisis gen 16S-rRNA, isolat bakteri KPU 2123 memiliki kemiripan 95% dengan Stanotrophomonas maltophilia. Enzim kitosanase dari isolat ini bekerja optimal pada suhu 50 ºC dan pH 6. Enzim ini cukup stabil pada suhu 37 ºC selama 120 menit. Penambahan ion logam berpengaruh terhadap aktivitas enzim. Ion logam Zn²+ (sebagai garam klorida 1 mM menghambat 100% aktivitas enzim tersebut. Penggunaan enzim kitosanase dalam menghidrolisis substrat kitosan, menghasilkan oligomer kitosan yang mengandung tetramer, pentamer dan heksamer Oligor kitosan tersebut mampu menghambat pertumbuhan bakteri Staphylococcus aureus sebesar 10,06% dan dapat menyebabkan kematian sel HeLa dengan LC50 pada dosis 120 ppm.

  5. Diversidade e potencial enzimático de fungos filamentosos isolados do solo, Semi-Árido, Pernambuco, Brasil

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    Oliveira, Luciana Gonçalves de

    2009-01-01

    Os fungos compreendem um grupo heterogêneo de microrganismos, atuando como sapróbios, parasitas e simbiontes. Como sapróbios, podem viver em diversos ambientes, especialmente no solo. Este trabalho teve como objetivos o isolamento, identificação e atividade enzimática (lacase e peroxidase) de fungos filamentosos do solo do Vale do Catimbau, semi-árido pernambucano. Quatro coletas de solo foram feitas durante os períodos chuvosos (2) e de estiagem (2). Foram identificadas 87 espécies de fungos...

  6. Transepithelial SCFA fluxes link intracellular and extracellular pH regulation of mouse colonocytes.

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    Chu, S; Montrose, M H

    1997-10-01

    We have studied pH regulation in both intracellular and extracellular compartments of mouse colonic crypts, using distal colonic mucosa with intact epithelial architecture. In this work, we question how transepithelial SCFA gradients affect intracellular pH (pHi) and examine interactions between extracellular pH (pHo) and pHi regulation in crypts of distal colonic epithelium from mouse. We studied pH regulation in three adjacent compartments of distal colonic epithelium (crypt lumen, crypt epithelial cell cytosol, and lamina propria) with SNARF-1 (a pH sensitive fluorescent dye), digital imaging microscopy (for pHi), and confocal microscopy (for pHo). Combining results from the three compartments allows us to find how pHi and pHo are regulated and related under the influence of physiological transepithelial SCFA gradients, and develop a better understanding of pH regulation mechanisms in colonic crypts. Results suggest a complex interdependency between SCFA fluxes and pHo values, which can directly affect how strongly SCFAs acidify colonocytes.

  7. Karakterisasi dan Aplikasi Enzim Transglutaminase dari Streptoverticillium ladakanum pada Daging Lumat IKan Mata Goyang

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    Yusro Nuri Fawzya

    2011-12-01

    Full Text Available Telah dilakukan karakterisasi enzim transglutaminase mikroba (MTGase yang diproduksi dari Streptoverticillium ladakanumdengan menggunakan media yang mengandung limbah cair tahu dan hidrolisat tepung tapioka. Enzim MTGase yang dikarakteris as i merupakan enzim kasar yang telah dipekatkan menggunakan ultrafiltrasi dan dikeringbekukan. Enzim ini kemudian diaplikasikan pada daging lumat ikan mata goyang (Priacanthus macracanthus lalu diamati sifat fisik (tekstur produk restrukturisasi yang dihasilkan. Sebagai pembanding,  dilakukan aplikasi TGase komersial (KTGase pada daging lumat yang sama. Penambahan TGase dilakukan dengan 2 cara, yaitu:  (1 bersama-sama dengan garam NaCl 1%, (2 bersama-sama dengan garam NaCl 1% dan sodium kaseinat 1%. Sebagai control adalah daging lumat ditambah garam N aCl 1% (tanpa penambahan enzim TGase. Hasil penelitian menunjukkan bahwa MTGase dari S. ladakanumbekerja optimum pada pH 8 dan suhu 55°C. Aktivitas enzim ini relatif tidak terpengaruh oleh adanya ion logam Ca2+,Mg2+, Na+, dan K+maupun inhibitor seperti ethylenediaminetetraacetic acid(EDTA, dan phenylmethyl-sulfonylfluoride(PMSF. Enzim MTGase tanpa penambahan sodium kaseinat menunjukkan kemampuan membentuk gel yang tidak berbeda dengan TGase komersial, menghasilkan kekuatan gel 16848 g mm dan nilai kekenyalan 0,97. Enzim ini juga terbukti dapat meningkatkan kekuatan gel, kekenyalan, dan kepadatan produk restrukturisasi daging lumat ikan yang hanya ditambah garam NaCl saja atau yang ditambah garam NaCl dan sodium kaseinat.

  8. Structural basis of dual Ca2+/pH regulation of the endolysosomal TRPML1 channel

    Energy Technology Data Exchange (ETDEWEB)

    Li, Minghui; Zhang, Wei K.; Benvin, Nicole M.; Zhou, Xiaoyuan; Su, Deyuan; Li, Huan; Wang, Shu; Michailidis, Ioannis E.; Tong, Liang; Li, Xueming; Yang, Jian

    2017-01-23

    The activities of organellar ion channels are often regulated by Ca2+ and H+, which are present in high concentrations in many organelles. Here we report a structural element critical for dual Ca2+/pH regulation of TRPML1, a Ca2+-release channel crucial for endolysosomal function. TRPML1 mutations cause mucolipidosis type IV (MLIV), a severe lysosomal storage disorder characterized by neurodegeneration, mental retardation and blindness. We obtained crystal structures of the 213-residue luminal domain of human TRPML1 containing three missense MLIV-causing mutations. This domain forms a tetramer with a highly electronegative central pore formed by a novel luminal pore loop. Cysteine cross-linking and cryo-EM analyses confirmed that this architecture occurs in the full-length channel. Structure–function studies demonstrated that Ca2+ and H+ interact with the luminal pore and exert physiologically important regulation. The MLIV-causing mutations disrupt the luminal-domain structure and cause TRPML1 mislocalization. Our study reveals the structural underpinnings of TRPML1's regulation, assembly and pathogenesis.

  9. Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi

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    Yasemin Kılıç

    2015-02-01

    Full Text Available Bu çalışmada, insan, gıda ve hayvan kaynaklı 39 Lactobacilllus cinsine ait ve yeni doğan gaitasından izole edilmiş 3 Bifidobacterium cinsine ait toplam 42 bakteri kullanılmıştır. O-nitrofenil-beta-D-galaktosit (o-NPG substrat olarak kullanılarak, kültürlerin β-galaktosidaz enzim ve spesifik aktiviteleri belirlenmiştir. Lactobacillus cinsine ait kültürlerden L. fermentum ZYN17 (2.468 U/mg, L. casei LB65 (1.116 U/mg, L. rhamnosus GD11 (1.034 U/mg ve L. acidophilus BAZ36 (0.947 U/mg suşlarının, Bifidobacterium cinsine ait kültürlerden de B. breve A26 (0.726 U/mg suşunun en yüksek spesifik aktivite yeteneğine sahip oldukları tespit edilmiştir. Ayrıca, bakterilerin 5-brom-4-klor-3-indolil-β-D-galaktopiranosit (X-gal substrat bileşiğiyle de nitel olarak enzim aktivitesinin varlığı değerlendirilmiştir. Yüksek spesifik β-galaktozidaz aktivitesi gösteren ZYN17 suşuna ait β-galaktozidaz enziminin optimizasyonu yapılmıştır. β-galaktozidaz enziminin optimum pH’sı 6.8, optimum sıcaklığı 37 ºC ve optimum tamponun potasyum fosfat tamponu olduğu belirlenmiştir.

  10. PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp.

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    Irzal Effendi

    2003-05-01

    Full Text Available Development of digestive enzymes; protease, lipase and amylase were observed in patin catfish, Pangasius hypophthalmus, larvae.  The 1 day old larvae (day after hatching, with 3,37-3,97 mm length and 0,62-0,79 mg weight, were reared in aquarium 60x50x40 cm with stocking  density of 20 fish/l.  Larvae were fed  Artemia dan tubificid worms 2-8 dan 7-15 days after hatching (dAH,  respectively (schedule I;  2-6 and  5-15 dAH (schedule II; and 2-4 and 5-15 dAH (schedule III.  Chlorella was ready to eat by larvae at the entirely rearing.  For enzyme assay, larvae were sampled from each aquarium at stages of 1, 2, 3, 5, 7, 10 and 15 dAH.    Protease and lipase activity were detected in digestive tract of  1 dAH larvae.   Digestive enzymes development have a similar pattern in larvae for all feeding schedules.  Protease activity  decreased with the increasing of age until 3 dAH, then increased  until the larvae reached 7 dAH, and sharply decreased until 10 dAH and then slowly decreased thereafter. Lipase activity tended to increase slowly with age up to 3 dAH, and increased sharply until 5 dAH, and then decreased sharply until 7 dAH  before decreased again up to the end of rearing.  Amylase activity in larvae increased slowly with the increasing of age up to 5 dAH, then increased sharply until 7 dAH, and decreased thereafter.  In dimly lighted larvae, amylase activity decreased before increased up to 12 d AH, then decreased thereafter.  The amount of food organisms in larval gut, body weight and length, and survival rate of larvae were also measured and discussed.Key Words:  Digestive enzymes, development, larvae, patin catfish, Pangasius hypophthalmus ABSTRAKPenelitian ini bertujuan untuk mengetahui perkembangan enzim protease, lipase dan amilase saluran pencernaan larva ikan patin akibat perubahan skedul pemberian pakan.  Larva ikan patin (panjang 3,77–3,97 mm dan bobot 0,62-0,79 mg berumur 1 hari dipelihara di akuarium 60x

  11. STUDI AKTIVITAS ENZIM PENCERNAAN LARVA IKAN KUWE, Gnathanodon speciosus YANG DIPELIHARA DENGAN JENIS PAKAN AWAL BERBEDA

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    Titiek Aslianti

    2012-04-01

    Full Text Available Upaya kontinuitas produksi benih ikan kuwe, Gnathanodon speciosus telah dilakukan namun sintasan yang diperoleh belum stabil. Jenis dan ukuran pakan pada larva stadia awal seringkali menjadi penyebab utama kegagalan produksi benih. Aktivitas enzim pencernaan diketahui sangat terkait dengan jenis pakan yang dikonsumsi larva sehingga berdampak terhadap pertumbuhan dan sintasannya. Enzim protease, amilase, dan lipase merupakan indikator biologis yang dapat menunjukkan kesesuaian jenis pakan yang dikonsumsi larva melalui kemampuannya untuk mencerna. Penelitian bertujuan untuk mengetahui aktivitas enzim pencernaan larva ikan kuwe yang diberi ransum pakan awal berbeda yaitu rotifer, gonad kerang, dan kuning telur ayam. Penelitian dilakukan dengan menggunakan rancangan acak lengkap 3 perlakuan dengan 3 ulangan. Hasil penelitian menunjukkan bahwa aktivitas enzim protease, amilase, dan lipase pada ketiga perlakuan mempunyai korelasi positif terhadap pertumbuhan. Aktivitas enzim pencernaan cenderung meningkat pada saat larva mulai menerima pakan eksogen (D-2, kemudian menurun pada D-3–D-7 selanjutnya relatif stabil hingga akhir penelitian (D-30. Pakan awal kuning telur menghasilkan pertumbuhan (TL = 13,3±1,77 mm dan sintasan benih (55,42% paling tinggi daripada rotifer (TL = 10,6±1,51 mm; SR 52,42% maupun gonad kerang (TL = 12,7±2,67 mm; 52,45%. Hasil penelitian diharapkan dapat meningkatkan produksi benih sebagai pasok yang kontinu dalam mendukung pengembangan budidaya.

  12. SELEKSI BAKTERI METHANOTROF (PEREDUKSI EMISI GAS METAN DI LAHAN SAWAH BERDASARKAN AKTIVITAS ENZIM METHAN MONOOKSIGENASE

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    Maimuna Nonci

    2016-03-01

    Full Text Available Kehadiran bakteri metanotrof pada daerah rhizosfer padi sangat dibutuhkan untuk mereduksi metan yang dihasilkan oleh bakteri metanogen, sehingga tidak terjadi emisi gas metan ke atmosfer. Penelitian ini bertujuan untuk melakukan koleksi dan seleksi bakteri methanotrof asal rhizosfer tanaman padi yang mampu menghasilkan enzim metan monooksigenase. Pengambilan sampel dilakukan secara acak pada tiga fase pertumbuhan tanaman padi yaitu fase vegetatif, reproduktif dan pematangan. Koleksi isolat dilakukan dengan metode isolasi dan pemurnian. Seleksi isolat dilakukan dengan pengujian reaksi gram, motil, indol, aerob/anaerob dan aktivitas enzim monooksigenase dianalisis dengan metode kolorimetrik.  Hasil penelitian diperoleh 52 koleksi isolat bakteri. Berdasarkan reaksi gram terseleksi 22 isolat gram negatif, berdasarkan analisis kolorimetrik  diperoleh 10 isolat  menghasilkan enzim metan monooksigenase.

  13. Constant pH Accelerated Molecular Dynamics Investigation of the pH Regulation Mechanism of Dinoflagellate Luciferase.

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    Donnan, Patrick H; Ngo, Phong D; Mansoorabadi, Steven O

    2018-01-23

    The bioluminescence reaction in dinoflagellates involves the oxidation of an open-chain tetrapyrrole by the enzyme dinoflagellate luciferase (LCF). The activity of LCF is tightly regulated by pH, where the enzyme is essentially inactive at pH ∼8 and optimally active at pH ∼6. Little is known about the mechanism of LCF or the structure of the active form of the enzyme, although it has been proposed that several intramolecularly conserved histidine residues in the N-terminal region are important for the pH regulation mechanism. Here, constant pH accelerated molecular dynamics was employed to gain insight into the conformational activation of LCF induced by acidification.

  14. Role of Sodium Bicarbonate Cotransporters in Intracellular pH Regulation and Their Regulatory Mechanisms in Human Submandibular Glands.

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    Namkoong, Eun; Shin, Yong-Hwan; Bae, Jun-Seok; Choi, Seulki; Kim, Minkyoung; Kim, Nahyun; Hwang, Sung-Min; Park, Kyungpyo

    2015-01-01

    Sodium bicarbonate cotransporters (NBCs) are involved in the pH regulation of salivary glands. However, the roles and regulatory mechanisms among different NBC isotypes have not been rigorously evaluated. We investigated the roles of two different types of NBCs, electroneutral (NBCn1) and electrogenic NBC (NBCe1), with respect to pH regulation and regulatory mechanisms using human submandibular glands (hSMGs) and HSG cells. Intracellular pH (pHi) was measured and the pHi recovery rate from cell acidification induced by an NH4Cl pulse was recorded. Subcellular localization and protein phosphorylation were determined using immunohistochemistry and co-immunoprecipitation techniques. We determined that NBCn1 is expressed on the basolateral side of acinar cells and the apical side of duct cells, while NBCe1 is exclusively expressed on the apical membrane of duct cells. The pHi recovery rate in hSMG acinar cells, which only express NBCn1, was not affected by pre-incubation with 5 μM PP2, an Src tyrosine kinase inhibitor. However, in HSG cells, which express both NBCe1 and NBCn1, the pHi recovery rate was inhibited by PP2. The apparent difference in regulatory mechanisms for NBCn1 and NBCe1 was evaluated by artificial overexpression of NBCn1 or NBCe1 in HSG cells, which revealed that the pHi recovery rate was only inhibited by PP2 in cells overexpressing NBCe1. Furthermore, only NBCe1 was significantly phosphorylated and translocated by NH4Cl, which was inhibited by PP2. Our results suggest that both NBCn1 and NBCe1 play a role in pHi regulation in hSMG acinar cells, and also that Src kinase does not regulate the activity of NBCn1.

  15. Role of Sodium Bicarbonate Cotransporters in Intracellular pH Regulation and Their Regulatory Mechanisms in Human Submandibular Glands.

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    Eun Namkoong

    Full Text Available Sodium bicarbonate cotransporters (NBCs are involved in the pH regulation of salivary glands. However, the roles and regulatory mechanisms among different NBC isotypes have not been rigorously evaluated. We investigated the roles of two different types of NBCs, electroneutral (NBCn1 and electrogenic NBC (NBCe1, with respect to pH regulation and regulatory mechanisms using human submandibular glands (hSMGs and HSG cells. Intracellular pH (pHi was measured and the pHi recovery rate from cell acidification induced by an NH4Cl pulse was recorded. Subcellular localization and protein phosphorylation were determined using immunohistochemistry and co-immunoprecipitation techniques. We determined that NBCn1 is expressed on the basolateral side of acinar cells and the apical side of duct cells, while NBCe1 is exclusively expressed on the apical membrane of duct cells. The pHi recovery rate in hSMG acinar cells, which only express NBCn1, was not affected by pre-incubation with 5 μM PP2, an Src tyrosine kinase inhibitor. However, in HSG cells, which express both NBCe1 and NBCn1, the pHi recovery rate was inhibited by PP2. The apparent difference in regulatory mechanisms for NBCn1 and NBCe1 was evaluated by artificial overexpression of NBCn1 or NBCe1 in HSG cells, which revealed that the pHi recovery rate was only inhibited by PP2 in cells overexpressing NBCe1. Furthermore, only NBCe1 was significantly phosphorylated and translocated by NH4Cl, which was inhibited by PP2. Our results suggest that both NBCn1 and NBCe1 play a role in pHi regulation in hSMG acinar cells, and also that Src kinase does not regulate the activity of NBCn1.

  16. Variability in larval gut pH regulation defines sensitivity to ocean acidification in six species of the Ambulacraria superphylum.

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    Hu, Marian; Tseng, Yung-Che; Su, Yi-Hsien; Lein, Etienne; Lee, Hae-Gyeong; Lee, Jay-Ron; Dupont, Sam; Stumpp, Meike

    2017-10-11

    The unusual rate and extent of environmental changes due to human activities may exceed the capacity of marine organisms to deal with this phenomenon. The identification of physiological systems that set the tolerance limits and their potential for phenotypic buffering in the most vulnerable ontogenetic stages become increasingly important to make large-scale projections. Here, we demonstrate that the differential sensitivity of non-calcifying Ambulacraria (echinoderms and hemichordates) larvae towards simulated ocean acidification is dictated by the physiology of their digestive systems. Gastric pH regulation upon experimental ocean acidification was compared in six species of the superphylum Ambulacraria. We observed a strong correlation between sensitivity to ocean acidification and the ability to regulate gut pH. Surprisingly, species with tightly regulated gastric pH were more sensitive to ocean acidification. This study provides evidence that strict maintenance of highly alkaline conditions in the larval gut of Ambulacraria early life stages may dictate their sensitivity to decreases in seawater pH. These findings highlight the importance of identifying and understanding pH regulatory systems in marine larval stages that may contribute to substantial energetic challenges under near-future ocean acidification scenarios. © 2017 The Author(s).

  17. Deletion of pH Regulator pac-3 Affects Cellulase and Xylanase Activity during Sugarcane Bagasse Degradation by Neurospora crassa.

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    Amanda Cristina Campos Antoniêto

    Full Text Available Microorganisms play a vital role in bioethanol production whose usage as fuel energy is increasing worldwide. The filamentous fungus Neurospora crassa synthesize and secrete the major enzymes involved in plant cell wall deconstruction. The production of cellulases and hemicellulases is known to be affected by the environmental pH; however, the regulatory mechanisms of this process are still poorly understood. In this study, we investigated the role of the pH regulator PAC-3 in N. crassa during their growth on sugarcane bagasse at different pH conditions. Our data indicate that secretion of cellulolytic enzymes is reduced in the mutant Δpac-3 at alkaline pH, whereas xylanases are positively regulated by PAC-3 in acidic (pH 5.0, neutral (pH 7.0, and alkaline (pH 10.0 medium. Gene expression profiles, evaluated by real-time qPCR, revealed that genes encoding cellulases and hemicellulases are also subject to PAC-3 control. Moreover, deletion of pac-3 affects the expression of transcription factor-encoding genes. Together, the results suggest that the regulation of holocellulase genes by PAC-3 can occur as directly as in indirect manner. Our study helps improve the understanding of holocellulolytic performance in response to PAC-3 and should thereby contribute to the better use of N. crassa in the biotechnology industry.

  18. KARAKTERISASI ENZIM POLIFENOL OKSIDASE BIJI KAKAO (Theobroma cacao Linn. Characterization of Polyphenol Oxidase Enzyme of Cocoa Beans (Theobroma cacao Linn.

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    G.P. Ganda Putra

    2012-05-01

    max equals to 595,24 U/gram (db of cocoa beans and K equals to 0,20 M; result of SDS- PAGE of enzyme shows protein bands at M� of 11,75; 17,80; 27,80; 36,03 and 131,52 kDa; and finally, optimumcondition of enzyme activity is at the temperature of 53,43oC; pH 5,42 and incubation time of 80,91 minutes. ABSTRAK Telah dilakukan penelitian dengan tujuan untuk: (1 mengetahui karakteristik enzim polifenol oksidase dan (2 menen- tukan kondisi (suhu, pH dan waktu inkubasi optimum aktivitas enzim polifenol oksidase, yang diisolasi dari biji kakao kering. Hasil penelitian berupa kondisi (suhu, pH dan waktu inkubasi optimum aktivitas enzim polifenol oksidase akan diaplikasikan untuk perbaikan mutu biji kakao kering melalui teknik rehidrasi. Penelitian ini dilakukan melalui tahapan: (1 isolasi enzim polifenol oksidase dari 10 sampel biji kakao kering di Provinsi Bali, (2 karakterisasi enzim polifenol oksidase (aktivitas, kinetika enzim, BM dan (3 penentuan kondisi suhu, pH dan waktu inkubasi optimum. Hasil yang diperoleh menunjukkan bahwa: (1 aktivitas enzim polifenol oksidase rata-rata 157,49 ± 58,03 U/gram (bk biji kakao dengan aktivitas maksimum 258,22 U/gram (bk biji kakao dan minimum 59,01 U/gram (bk biji kakao; maks sebesar 595,24 U/gram (bk biji kakao dan K sebesar 0,20 M; hasil SDS-PAGE enzim menunjukkan pita-pita protein yang terbentuk pada BM 11,75; 17,80; 27,80; 36,03 dan 131,52 kDa; dan (2 kondisi optimum aktivitas enzimadalah suhu 53,43oC; pH 5,42 dan waktu inkubasi 80,91 menit.

  19. Blanqueig enzimàtic de pasta de kenaf emprant sistemes lacasa-mediador

    OpenAIRE

    Andreu Terrén, Glòria

    2013-01-01

    Premi extraordinari doctorat curs 2012-2013, àmbit d’Enginyeria Industrial La present tesi s’emmarca dins d’una de les línies del Grup de Recerca Paperer i Gràfic (CIPAGRAF), del Departament d’Enginyeria Tèxtil i Paperera de l’Escola Tècnica Superior d’Enginyeries Industrial i Aeronàutica de Terrassa, encaminada a l’aplicacióde la Biotecnologia per desenvolupar nous sistemes enzimàtics per al blanqueig depastes i modificació de fibres cel·lulòsiques. La implantació d’etapes enzimàtiques...

  20. Aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang

    Directory of Open Access Journals (Sweden)

    Dyah Widiastuti

    2015-06-01

    Full Text Available Abstract. Dengue Haemorrhagic Fever (DHF is a major health problem in Tembalang sub district, Semarang City. Fogging with insecticide applications was done frequently as an effort to control Dengue vectors. The use of insecticides from the same class in a long time can lead to resistance in mosquitos’ population. The research aimed to observe the activity of monooxygenases in Aedes aegypti populations in Tembalang Subdistrict, Semarang. The study was conducted during February-November 2014 with a cross-sectional design in 10 villages in Tembalang Subdistirict, Semarang City. Field strains of Ae. aegypti eggs were collected using ovitraps. The collected eggs were grown under standard condition to adult mosquitoes. Mosquitos’ homogenate were stored at -85°C and used for biochemical assays. The results showed there was increased monooxygenases activity in Ae. aegypti populations. Resistance to synthetic pyrethroid insecticide in Ae. aegypti mosquitoes population in Tembalang Subdistrict might be caused by the mechanism of detoxification enzymes in particular monooxygenases Keywords: monooxygenase, insecticide, Ae. aegypti, resistance  Abstrak. Demam Berdarah Dengue (DBD masih menjadi masalah kesehatan utama di Kecamatan Tembalang, Kota Semarang. Tindakan fogging untuk pengendalian vektor DBD sering dilakukan. Penggunaan insektisida dari golongan yang sama dalam waktu cukup lama dapat memicu terjadinya resistensi. Tujuan penelitian untuk mengamati aktivitas enzim monooksigenase pada populasi nyamuk Aedes aegypti di Kecamatan Tembalang, Kota Semarang. Penelitian dilaksanakan bulan Februari-November 2014 dengan desain potong lintang di 10 desa di Kecamatan Tembalang, Kota Semarang. Pada penelitian ini dilakukan pemasangan ovitrap untuk mendapatkan sampel telur yang dipelihara menjadi nyamuk dewasa. Sampel homogenate nyamuk disimpan pada suhu -85°C, selanjutnya dilakukan peng-ujian resistensi dengan uji biokimia untuk melihat aktivitas enzim

  1. Ambient Space and Ambient Sensation

    DEFF Research Database (Denmark)

    Schmidt, Ulrik

    The ambient is the aesthetic production of the sensation of being surrounded. As a concept, 'ambient' is mostly used in relation to the music genre 'ambient music' and Brian Eno's idea of environmental background music. However, the production of ambient sensations must be regarded as a central...... aspect of the aesthetization of modern culture in general, from architecture, transport and urbanized lifeforms to film, sound art, installation art and digital environments. This presentation will discuss the key aspects of ambient aesthetization, including issues such as objectlessness...

  2. Enzim Papain: Aspek Green Chemistry pada Reaksi Knoevenagel

    Directory of Open Access Journals (Sweden)

    Laurentius Haryanto

    2015-08-01

    Full Text Available Green chemistry aspect is the chemical approach that has been studied in the past two decades. One of the principles is the development of green synthesis process that is friendly for the environment. This research showed that papain can be used as catalyst for Knoevenagel reaction with 3 kinds of substituted-benzaldehyde and malononitrile as substrates in aqueous medium. The best reaction condition with 80% yield was reached by utilizing of 25 mg papain/mmol substrate. Reaction was conducted at ambient temperature and pressure for 30 min. Products were yellowish to yellow needle crystals and successfully characterized by melting point, UV-Vis, IR, mass spectra, and 13C & 1H-NMR, named as 2-(4-hydroxybenzylidene-malononitrile; 2-(3-hydroxybenzylidene-malononitrile; and 2-(4-hydroxy-3-methoxybenzylidene-malononitrile.

  3. Det ambiente

    DEFF Research Database (Denmark)

    Schmidt, Ulrik

    Om begrebet "det ambiente", der beskriver, hvad der sker, når vi fornemmer baggrundsmusikkens diskrete beats, betragter udsigten gennem panoramavinduet eller tager 3D-brillerne på og læner os tilbage i biografsædet. Bogen analyserer, hvorfan ambiente oplevelser skabes, og hvilke konsekvenser det...

  4. Ambient Sensors

    NARCIS (Netherlands)

    Börner, Dirk; Specht, Marcus

    2014-01-01

    This software sketches comprise two custom-built ambient sensors, i.e. a noise and a movement sensor. Both sensors measure an ambient value and process the values to a color gradient (green > yellow > red). The sensors were built using the Processing 1.5.1 development environment. Available under

  5. FRAKSINASI ENZIM LIPASE DARI ENDOSPERM KELAPA DENGAN METODE SALTING OUT (Lipase fractionation of Coconut Endosperm by Salting out Method

    Directory of Open Access Journals (Sweden)

    Moh. Su'i

    2014-02-01

    Full Text Available This research learns about fractionation of lipases activity from coconut endosperm by using ammonium sulphate of 0–15%; 15-30 %, 30–45 %, 45–60 %, 60–75 % and 75–90 %. The results showed that the fractions of 0–15% ; 30–45 %, 45–60 % and 60–75 % have lipase activity. Meanwhile, the highest activity was fractions of 60-75%. fractions of 15-30% and 75-90%  have no lipase enzym activity. Molecule weigh of lipase enzyme was 72 kDa. Keywords: Lipases, endosperm, coconut, fractionation, ammonium sulphate   ABSTRAK Penelitian ini mempelajari fraksinasi enzim lipase dari endosperm kelapa menggunakan ammonium sulfat. fraksinasi dilakukan dengan variasi konsentrasi ammonium sulfat 0–15% ; 15-30%; 30–45 %, 45–60 %, 60–75 % dan 75–90 %. Hasil penelitian menunjukkan bahwa enzim lipase terdapat pada fraksi 0–15% ; 30–45 %, 45–60 % dan fraksi 60–75 % dengan aktivitas enzim tertinggi pada fraksi 60-75%. Sedangkan fraksi 15-30% dan 75-90% tidak ada enzim lipase. Berat molekul enzim lipase pada semua fraksi 72 kDa. Kata kunci: Lipase, endosperm, fraksinasi, ammonium sulfat

  6. Det Ambiente

    DEFF Research Database (Denmark)

    Schmidt, Ulrik

    Det ambiente er iscenesættelsen af en karakteristisk sanseoplevelse, der er kendetegnet ved fornemmelsen af at være omgivet. I dag bliver begrebet om det ambiente mest anvendt i forbindelse med musikgenren ’ambient musik’. Det ambiente er dog ikke essentielt knyttet til det musikalske, men må...... forstås som et betydeligt bredere fænomen i den moderne æstetiske kultur, der spiller en væsentlig rolle i oplevelsen af moderne transportformer, arkitektur, film, lydkunst, installationskunst og digitale multimedieiscenesættelser. En forståelse af det ambiente er derfor centralt for forståelsen af en...... moderne æstetiseret oplevelseskultur i almindelighed. Da det ambiente ikke hidtil har været gjort til genstand for en mere indgående teoretisk behandling, er der dog stor usikkerhed omkring, hvad fænomenet overhovedet indebærer. Hovedformålet med Det ambiente – Sansning, medialisering, omgivelse er derfor...

  7. Caracterización y fotoestabilidad de compuestos fluorescentes y pardos generados en reacciones enzimáticas y no enzimáticas. Implicancias en la conservación de materiales orgánicos

    OpenAIRE

    Matiacevich, Silvia Beatriz

    2008-01-01

    Las reacciones de pardeamiento no enzimático (reacción de Maillard) y enzimático (polimerización de polifenoles oxidados) generan productos fluorescentes y pigmentos pardos que contribuyen a las características físico-químicas de materiales orgánicos (biomoléculas, alimentos, fármacos). El objetivo de esta tesis fue analizar las características cromáticas y de fluorescencia y la fotoestabilidad de los compuestos generados por la reacción de Maillard y de pigmentos polifenólicos, para dilucida...

  8. Kemampuan Isolataktinomisetes Menghasilkan Enzim yang Dapatmerusak Kulit Telur Nematoda Puru-Akar Meloidogyne spp.

    Directory of Open Access Journals (Sweden)

    Bambang Rahayu TP

    2009-07-01

    Full Text Available Soil microbes including actinomycetes are known to produce various hydrolytic enzymes and antibiotics that can be used as biological controlling agents nematode. Therefore, surveys conducted in several areas in Yogyakarta, Central Java and East Java, to search for actinomycetes with chitinolytic, proteolytic, and chitino-proteolytic activity. Isolation of Actinomycetes produced 84 isolates, and most was obtained from shrimp head waste (26 isolates. After the selection based on their ability to hydrolyze chitines and protein in the medium, those whith the highest chitin and protein hydrolysis activity, are consecutive PSJ 27, TL 8, and TL 10 isolates. Test results of crude enzyme produced by selected isolates against root-knot nematode eggshell, showed that the isolates that have chitino-proteolytic activity (TL 10, is a highly effective isolate in damage eggshell. There are three types of damage to the nematode eggs. In the young eggs, crude enzyme preparation causing damage on vitelline and chitin layers. In the older eggs, preparation of crude enzyme cause premature hatching.   Sebagian mikrobia tanah, termasuk aktinomisetes, diketahui mampu menghasilkan berbagai enzim hidrolitik dan antibiotik yang dapat dimanfaatkan sebagai agens pengendalian hayati nematoda. Oleh karena itu,survei dilakukan di beberapa daerah di Yogyakarta, Jawa Tengah, dan Jawa Timur untuk mencari aktinomisetes yang mempunyai aktivitas kitinolitik, proteolitik dan kitino-proteolitik. Isolasi aktinomisetes menghasilkan 84 isolat, dan yang terbanyak diperoleh dari limbah kepala udang (26 isolat. Setelah dilakukan seleksi berdasarkan kemampuannya menghidrolisis kitin dan protein dalam medium, yang mempunyai aktivitas hidrolisis protein, kitin, protein dan kitin tertinggi berturut-turut adalah isolat PSJ 27, TL 8, dan TL 10. Hasil uji enzim kasar yang dihasilkan isolat terpilih terhadap perusakan kulit telur nematoda puru-akar menunjukkan bahwa isolat yang memiliki

  9. Síntese enzimática de ascorbil oleato utilizando tecnologias alternativas

    OpenAIRE

    Balen, Manuela

    2016-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro Tecnológico, Programa de Pós-Graduação em Engenharia de Alimentos, Florianópolis, 2016. A produção enzimática de ascorbil oleato utilizando como substratos Lascórbico e ácido oleico necessita da total solubilização entre substratos a fim de se obter maiores rendimentos. Contudo, o ácido L-ascórbico e ácido oleico não são totalmente miscíveis por possuírem polaridades bastante distintas. Assim, este trabalho objetivou bus...

  10. Estudio de la cinética de la hidrólisis enzimática del bagazo pretratado

    OpenAIRE

    Albernas Carvajal, Yailet; Corsano, Gabriela; Mesa Garriga, Leyanis; Santos Herrero, Ronaldo; Gonzalez Suarez, Erenio

    2016-01-01

    En el estudio se determinan los parámetros fundamentales de la cinética enzimática a partir de un modelo cinético pseudo-homogéneo de Michaelis Menten, previamente estudiado con otros materiales lignocelulósicos, para determinar la velocidad de producción de Azúcares Reductores Totales (ART) en el tiempo. Para ello se emplearon los resultados obtenidos a nivel de laboratorio, en el cual se llevó a cabo la hidrólisis enzimática del bagazo previamente pretratado de forma ácida y básica. Se empl...

  11. Evaluación de complejos enzimáticos en alimentación de pollos de engorde

    OpenAIRE

    García Jiménez, Mario

    2011-01-01

    El objetivo de esta tesis doctoral ha sido evaluar la adición de diversos complejos enzimáticos a dietas basadas en cereales que contenían diferentes cantidades de polisacáridos no amiláceos (PNA), sobre la productividad y parametros digestivos de pollos de engorde. Asimismo se ha estudiado la variación de la respuesta a la suplementación enzimática(SE) en función de otros factores tales como el periodo de ayuno posterior al nacimiento, el procesamiento termico del cereal, el tipo de cereal u...

  12. Estudio de la cinética de la hidrólisis enzimática del bagazo pretratado

    OpenAIRE

    Albernas-Carvajal, Yailet; Corsano, Gabriela; Mesa Garriga, Layanis; Santos Herrero, Ronaldo; González Suárez, Erenio

    2015-01-01

    En el estudio se determinan los parámetros fundamentales de la cinética enzimática a partir de un modelo cinético pseudo-homogéneo de Michaelis Menten, previamente estudiado con otros materiales lignocelulósicos, para determinar la velocidad de producción de Azúcares Reductores Totales (ART) en el tiempo. Para ello se emplearon los resultados obtenidos a nivel de laboratorio, en el cual se llevó a cabo la hidrólisis enzimática del bagazo previamente pretratado de forma ácida y básica. Se empl...

  13. AKTIVITAS ENZIM KOMERSIAL, EKSTRAK KASAR ENZIM DARI VISCERA KEONG MAS (Pila polita, ABALON (Haliotis asinina, DAN BEKICOT (Achatina fulica UNTUK LISIS JARINGAN RUMPUT LAUT Kappaphycus alvarezii PADA KULTUR PROTOPLAS

    Directory of Open Access Journals (Sweden)

    Sri Redjeki Hesti Mulyaningrum

    2008-12-01

    Full Text Available Dalam usaha perbaikan kualitas bibit rumput laut Kappaphycus alvarezii dilakukan kultur protoplas dengan isolasi protoplas menggunakan enzim. Untuk mendapatkan sumber enzim yang ekonomis sebagai alternatif pengganti enzim komersial dan untuk mengetahui perbandingan konsentrasi enzim komersial yang optimum agar menghasilkan jumlah protoplas yang maksimum, dilakukan karakterisasi terhadap enzim dari berbagai sumber. Aktivitas ekstrak kasar enzim dari viscera bekicot (Achatina fulica tidak berbeda nyata dengan enzim komersial (P>0,05 dengan aktivitas sebesar 0,729 unit/mL; enzim komersial 0,354 unit/mL; ekstrak kasar enzim dari viscera keong mas (Pila polita 0,048 unit/mL; dan ekstrak kasar enzim dari viscera abalon (Haliotis asinina 0,014 unit/mL. Perbandingan enzim komersial yang optimum adalah 2:1 menghasilkan protoplas sebanyak 1,26 x 108 sel/mL; kemudian 1:2 dengan jumlah protoplas 1,22 x 108 sel/mL; perbandingan 1:1 menghasilkan protoplas sebanyak 8,36 x 107 sel/mL; perbandingan 0:1 menghasilkan protoplas sebanyak 6,33 x 107 sel/mL; dan perbandingan 1:0 menghasilkan protoplas sebanyak 9,55 x 106 sel/mL. Rumput laut asal Takalar memiliki protoplas dengan kepadatan tertinggi sebesar 3,7 x 108 sel/mL. Effort to improve the quality of seaweed seed Kappaphycus alvarezii has been done by protoplast culture with protoplast isolation using enzyme. To find out economical enzyme sources as alternatives to substitute the expensive commercial enzyme and to determine the optimum concentration ratio of commercial enzyme to produce maximum amount of protoplast, characterization was executed to several potential sources. Activity of crude extract enzyme from viscera of garden snail (Achatina fulica was not significantly different with commercial enzyme (P>0.05 it was 0.729 unit/mL, commercial enzyme 0.354 unit/mL activity; crude extract enzyme from viscera of golden snail (Pila polita 0.048 unit/mL activity and crude extract enzyme from viscera of abalone

  14. Periodismo ambiental

    Directory of Open Access Journals (Sweden)

    Lucía Lemos

    2015-01-01

    Full Text Available Los periodistas toman el tema del medio ambiente cada vez más en serio. El uso de temas relacionados con el medio ambiente, debe estar ligado al análisis socio-económico y a las posibilidades de comunicación y educación de diferentes regiones del mundo. A continuación se presenta un resumen de la situación ambiental, las acciones de prensa y comunicación que se llevan a cabo en América Central (Panamá, El Salvador, Costa Rica y en Sudamérica Brasil,Colombia, Chile, México, y Perú. Se concluye en la necesidad de formar hábitos ecológicos. Los comunicadores deben presentar soluciones a los problemas, fomentar campañas comunes, compartir información y velar por el ambiente ambiente para que las generaciones futuras no tengan que perecer.

  15. Galactosemia: Diagnóstico precoz mediante estudio enzimático

    Directory of Open Access Journals (Sweden)

    Úrsula Carrillo Estrada

    2003-09-01

    Full Text Available Se presentan los resultados obtenidos del estudio realizado a un paciente masculino de 45 días de nacido, cuyo motivo de ingreso fue pérdida de peso, decaimiento, retraso psicomotor y crisis de hipoglucemia. Los síntomas comenzaron en el período neonatal y coincidieron con la introducción de la lactancia materna. En estudios realizados se constató en la orina la presencia de lactosa y galactosa. Se confirma el diagnóstico por estudio enzimático. La evolución clínica ha sido satisfactoria. El tratamiento dietético que excluía a los alimentos que contienen galactosa y lactosa fue de mucha importancia. Es el primer caso diagnosticado en Cuba mediante estudio enzimático.This paper presents the results of a study performed on a 45-day old male patient who was admitted to the hospital for weight loss, tiredness, psychomotor retardation and hypoglicemic crisis. The symptoms had begun in the neonatal period and had coincided with the introduction of breast feeding. The studies detected lactose and galactose in urine. The enzymatic study confirmed the diagnosis. The clinical recovery was satisfactory. The dietary treatment that excluded foods containing galactose and lactose was important and successful. He is the first case diagnosed on enzymatic study in Cuba.

  16. Ambient Utopia

    NARCIS (Netherlands)

    Heylen, Dirk K.J.; Bosse, Tibor

    2012-01-01

    his chapter presents an analysis of the ambitions that lie behind the concept of Ambient Intelligence as it is presented by the advocates and researchers working in the field. In particular it looks at the ideas regarding the forms of natural and intuitive forms of interaction that are envisaged –

  17. Aktivitas Inhibitor Enzim Pengubah Angiotensin (ACE dan Antioksidan Peptida Kolagen dari Teripang Gama (Stichopus variegatus

    Directory of Open Access Journals (Sweden)

    M. Habbib Khirzin

    2015-05-01

    Full Text Available Teripang merupakan salah satu echinodermata yang memiliki kandungan protein tinggi dan sekitar 70% dari proteinnya merupakan kolagen. Tujuan penelitian ini adalah untuk mengetahui aktivitas inhibitor Angiotensin Converting Enzyme (ACE dan antioksidan dari peptida kolagen teripang Gama (Stichopus variegatus. Ekstraksi kolagen dilakukan menggunakan asam asetat 0,5 M. Peptida kolagen diperoleh melalui hidrolisis kolagen menggunakan enzim pepsin dengan konsentrasi 0,1 U/g kolagen, selama 0; 30; 60; 90; 120; 180; dan 240 menit. Aktivitas inhibitor ACE dan antioksidan peptida kolagen diuji dengan metode spektroskopi. Kolagen yang dihasilkan memiliki rendemen 16,40% dengan berat molekul 130,33 kDa. Aktivitas inhibitor ACE tertinggi dihasilkan dari proses hidrolisis selama 180 menit dengan penghambatan sebesar 82,31%, sedangkan aktivitas antioksidan tertinggi dihasilkan oleh peptida kolagen dari hidrolisis kolagen selama 120 menit dengan nilai IC50 1,9 mg/ml.

  18. AKTIVITAS HIDROLISIS ENZIM LIPASE DARI KENTOS KELAPA TERHADAP MINYAK KELAPA Hidrolysis Activity of Lipase Enzyme from Coconut Houstorium for Coconut Oil

    Directory of Open Access Journals (Sweden)

    Mohammad Su’i

    2012-05-01

    Full Text Available This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show  that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v and hydrolysis for 60 minutes with stirring. ABSTRAK Penelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v, lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis.

  19. Oxygen limitation modulates pH regulation of catabolism and hydrogenases, multidrug transporters, and envelope composition in Escherichia coli K-12

    Directory of Open Access Journals (Sweden)

    Radmacher Michael D

    2006-10-01

    Full Text Available Abstract Background In Escherichia coli, pH regulates genes for amino-acid and sugar catabolism, electron transport, oxidative stress, periplasmic and envelope proteins. Many pH-dependent genes are co-regulated by anaerobiosis, but the overall intersection of pH stress and oxygen limitation has not been investigated. Results The pH dependence of gene expression was analyzed in oxygen-limited cultures of E. coli K-12 strain W3110. E. coli K-12 strain W3110 was cultured in closed tubes containing LBK broth buffered at pH 5.7, pH 7.0, and pH 8.5. Affymetrix array hybridization revealed pH-dependent expression of 1,384 genes and 610 intergenic regions. A core group of 251 genes showed pH responses similar to those in a previous study of cultures grown with aeration. The highly acid-induced gene yagU was shown to be required for extreme-acid resistance (survival at pH 2. Acid also up-regulated fimbriae (fimAC, periplasmic chaperones (hdeAB, cyclopropane fatty acid synthase (cfa, and the "constitutive" Na+/H+ antiporter (nhaB. Base up-regulated core genes for maltodextrin transport (lamB, mal, ATP synthase (atp, and DNA repair (recA, mutL. Other genes showed opposite pH responses with or without aeration, for example ETS components (cyo,nuo, sdh and hydrogenases (hya, hyb, hyc, hyf, hyp. A hypF strain lacking all hydrogenase activity showed loss of extreme-acid resistance. Under oxygen limitation only, acid down-regulated ribosome synthesis (rpl,rpm, rps. Acid up-regulated the catabolism of sugar derivatives whose fermentation minimized acid production (gnd, gnt, srl, and also a cluster of 13 genes in the gadA region. Acid up-regulated drug transporters (mdtEF, mdtL, but down-regulated penicillin-binding proteins (dacACD, mreBC. Intergenic regions containing regulatory sRNAs were up-regulated by acid (ryeA, csrB, gadY, rybC. Conclusion pH regulates a core set of genes independently of oxygen, including yagU, fimbriae, periplasmic chaperones, and nhaB. Under oxygen limitation, however, pH regulation is reversed for genes encoding electron transport components and hydrogenases. Extreme-acid resistance requires yagU and hydrogenase production. Ribosome synthesis is down-regulated at low pH under oxygen limitation, possibly due to the restricted energy yield of catabolism. Under oxygen limitation, pH regulates metabolism and transport so as to maximize alternative catabolic options while minimizing acidification or alkalinization of the cytoplasm.

  20. ANALISA KADAR PROTEIN CRUDE ENZIM SELULASE DARI KAPANG Rhizopuz Sp PADA SUBSTRAT AMPAS TEBU HASIL ISOLASI DARI KEBUN CENGKEH, KARE, MADIUN

    Directory of Open Access Journals (Sweden)

    Pujiati pujiati

    2017-01-01

    Full Text Available Kapang Rhizopus sp merupakan salah satu mikroorganisme yang memiliki kemampuan tinggi untuk menghasilkan enzim selulase.Enzim selulase merupakan enzim yang dapat menghidrolisis selulosa. Hidrolisis meliputi proses pemecahan polisakarida di dalam biomassa lignoselulosa, yaitu: selulosa dan hemiselulosa menjadi monomer gula penyususnnya. Penelitian ini bertujuan untuk mengetahui produksi dan aktivitas enzim selulase terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp dengan subsrtat ampas tebu (bagase. Metode penelitian menggunakan kuantitatif eksperimen dengan pola rancangan acak lengkap (RAL dua faktorial. Perlakuan penelitian meliputi perbedan inokulum (K yaitu 5% (K1, 15% (K2, 25% (K3 dan lama fermentasi (T yaitu 3hari (T1, 6hari (T2, 9hari (T3, dan 12hari (T4. Data yang diambil dari perlakuan tersebut adalah kadar protein dengan metode brownstead lowry. Analisis data menggunakan variansi anava dua jalur dengan taraf signifikansi 5% setelah itu dilanjutkan dengan uji Beda Nyata Terkecil (BNT . Hasil penelitian menunjukkan bahwa: Fhit > Ftab sehingga ada pengaruh antara konsentrasi inokulum dan lama fermentasi terhadap aktivitas crude enzim selulase dari kapang Rhizopus sp, Perlakuan perbedaan konsentrasi dan lama fermentasi mendapatkan kadar protein tertinggi 0,715 dengan konsentrasi 25%  dan lama fementasi 25%

  1. Ambient diagnostics

    CERN Document Server

    Cai, Yang

    2014-01-01

    Part I. FundamentalsIntroductionWhat is Ambient Diagnostics?Diagnostic ModelsMultimedia IntelligenceCrowd SourcingSoft SensorsScience of SimplicityPersonal DiagnosesBasic AlgorithmsBasic ToolsSummaryProblemsTransformationEarly Discoveries of Heartbeat PatternsTransforms, Features, and AttributesSequential FeaturesSpatiotemporal FeaturesShape FeaturesImagery FeaturesFrequency Domain FeaturesMulti-Resolution FeaturesSummaryProblemsPattern RecognitionSimilarities and DistancesClustering MethodsClassification MethodsClassifier Accuracy MeasuresSummaryProblemsPart II. Multimedia IntelligenceSound RecognitionMicrophone AppsModern Acoustic Transducers (Microphones)Frequency Response CharacteristicsDigital Audio File FormatsHeart Sound SensingLung Sound SensingSnore MeterSpectrogram (STFT)Ambient Sound AnalysisSound RecognitionRecognizing Asthma SoundPeak ShiftFeature CompressionRegroupingNoise IssuesFuture ApplicationsSummaryProblemsColor SensorsColor SensingHuman Color VisionColor SensorsColor Matching ExperimentsC...

  2. Determinación de la actividad enzimática de la hexosaminidasa en fluidos biológicos

    Directory of Open Access Journals (Sweden)

    Marlén Rodríguez

    1996-12-01

    Full Text Available Este estudio describe un método electroforético que emplea el sustrato fluorogénico 4- metilumbelliferil 2-acetamida 2-deoxi-O-D-glucopiranosido, para la determinación enzimática de la hexosaminidasa (HEX en linfocitos de sangre periférica, líquido amniótico, suero y orina. Se propone que la electroforésis sirva como método confirmatorio del diagnóstico de la gangliosidosis GM2, ya que permite determinar la actividad enzimática de la HEX y hacer la diferenciación de las isoenzimas (HEX A y HEX 8. La deficiencia de estas isoenzimas se detecta por disminución o ausencia de fluorescencia.

  3. Modelado de la extracción con hexano de molido de soja pretratado enzimáticamente

    Directory of Open Access Journals (Sweden)

    Zaritzky, N

    2007-06-01

    Full Text Available In this work, the influence of the variables which can affect oil extraction from soybean enzymatically pretreated grit using hexane, were studied. From the experimental results, a mathematical model for calculating the extraction yield was proposed. The statistical tests used to obtain this model showed that the yield does not depend on the enzymatic incubation pH but on the temperature and time of enzymatic treatment and on the interaction between time and temperature. The proposed model fit the experimental data well.En este trabajo se estudia la influencia de las variables de proceso que pueden tener efecto sobre el rendimiento de la extracción con hexano a partir de soja molida pretratada enzimáticamente.Se propone a partir de los resultados experimentales, un modelo matemático para el cálculo del rendimiento de la extracción. Los tests estadísticos utilizados para obtener este modelo demostraron que el rendimiento en aceite no depende del pH de la incubación enzimática mientras que si depende tanto de la temperatura como del tiempo de ataque enzimático sobre el molido de soja. El modelo ajustó satisfactoriamente los datos experimentales.

  4. EFEK PEMBERIAN VITAMIN E TERHADAP JUMLAH ERYTROSIT DAN AKTIVITAS ENZIM KATALASE TIKUS AKIBAT PAPARAN SINAR ULTRAVIOLET

    Directory of Open Access Journals (Sweden)

    Yustini Alioes

    2009-09-01

    Full Text Available AbstrakSinar ultra violet (UV selain mempunyai dampak positif terhadap kesehatan, UV juga dapat membahayakan kesehatan. Dampak negatif UV ini dirasakan oleh orang-orang yang terpapar sinar UV dalam jangka waktu lama, seperti nelayan dan petani. Radiasi UV tingkat sedang menyebabkan kulit kemerahan (eritema, sedangkan tingkat tinggi dapat menyebabkan perdarahan pada kulit.Penelitian ini bertujuan untuk mengetahui efek pemberian vitamin E terhadap jumlah erytrosit dan aktifitas enzim katalase tikus akibat paparan sinar ultra violet. Penelitian ini bersifat eksperimental dengan menggunakan tikus sebagai hewan coba, yang dilakukan di Laboratorium Biokimia Fakultas Kedokteran Universitas Andalas dari bulan September – Desember 2008. Populasi adalah 20 ekor tikus galur Wistar, umur ± 2 bulan dan berat badan 200-250 gram. Sampel diambil secara acak (simple random sampling dari populasi dan dibagi menjadi dua kelompok yaitu : Kelompok kontrol (Kelompok yang diradiasi UV 6 jam/hari selama 3 minggu tanpa pemberian vitamin E dan kelompok perlakuan (Kelompok yang diradiasi UV 6 jam/hari selama 3 minggu dengan pemberian vitamin E dosis 0.45 IU/kg BB/1 kali/hari. Hasil penelitian menunjukkan pada kelompok kontrol terjadi penurunan jumlah erytrosit dan aktifitas enzim katalase akibat paparan sinar UV. Jumlah Penurunan ini akan lebih sedikit setelah pemberian vitamin E.Efek pemberian vitamin E dapat menghambat penurunan jumlah erytrosit dan aktivitas enzim katalase tikus yang terpapar sinar ultraviolet.Kata Kunci : VitaminE, Erytrosit, KatalaseAbstract Ultraviolet (UV in addition to having a positive impact on health, UV can also endanger your health. UV negative impact is felt by the people who are exposed to UV light for long periods, such as fishermen and farmers. UV radiation levels are causing skin redness (erythema, whereas high levels can cause bleeding in the skin. This study aims to determine the effect. These are experimental studies using rats as

  5. PENGARUH TIMBAL ASETAT TERHADAP AKTIVITAS ENZIM KATALASE HATI TIKUS PUTIH JANTAN

    Directory of Open Access Journals (Sweden)

    Asterina Asterina

    2012-09-01

    Full Text Available AbstrakTimbal (Pb dapat ditemukan di berbagai media lingkungan seperti udara, air, debu dan tanah. Logam Pb atau bentuk persenyawaannya berasal dari pembakaran bahan bakar kendaraan bermotor, emisi industri dan dari penggunaan cat bangunan yang mengandung Pb. Toksisitas Pb menghambat sebahagian besar enzim yang terlibat dalam biosintesis heme dan merusak sel hatiTujuan studi ini adalah untuk melihat pengaruh timbal (Pb terhadap aktivitas enzim katalase hati tikus putih jantan. Desain penelitian ini adalah eksperimental menggunakan 25 ekor tikus putih jantan yang dibagi menjadi lima kelompok, yaitu kelompok kontrol, dan kelompok perlakuan dengan pemberian Pb asetat dengan dosis konsentrasi 5 mg, 10 mg , 20 mg, dan 40 mg/kg BB selama 26 hari.Hasil penelitian menunjukkan terjadinya penurunan rerata aktivitas katalase hati dan serum secara bermakna (p < 0,05, setelah pemberian Pb asetat selama 26 hari. Penurunan aktivitas katalase hati dan serum tikus secara bermakna (p < 0,05 terjadi antara kelompok kontrol dibandingkan dengan kelompok tikus yang diberi dosis 40 mg/kg BB.Kesimpulan yang dapat diambil dari studi ini ialah bahwa pemberian Pb asetat dapat menurunkan aktivitas katalase hati dan juga mempengaruhi aktivitas katalase serum tikus.Kata kunci : Pb asetat, katalaseAbstractLead (Pb can be found in various environmental media such as air, water, dust and dirt. Pb in the form of compounds derived from burning motor vehicle fuel, industrial emissions and from the use of paints containing Pb buildings. Pb toxicity partly inhibit the enzymes involved in the biosynthesis of heme and damage the liver cells.The purpose of this study was to observe the effect of lead (Pb on the enzyme activity of rat liver catalase white male. Experimental research design was used 25 white male rats were divided into five groups, namely the control group and theARTIKEL PENELITIAN180group treated with the administration of Pb acetate at a dose concentration of 5 mg, 10

  6. CO{sub 2} injection as pH regulator in refrigeration circuits; Inyeccion de CO{sub 2} como regulador de pH en circuitos de refrigeracion

    Energy Technology Data Exchange (ETDEWEB)

    Belinchon, J.

    2009-07-01

    Water from rivers is not usually suitable for use in cooling circuits of industrial installations, normally because of its high fouling ability. this requires a treatment before use based on the use of fouling inhibitors, biocides and pH regulators. The most commonly used pH regulator is sulfuric acid, a very hazardous chemical product that also generates secondary saline contamination due to the sulfates. Since 2008, Iberdrola Generation, in collaboration with Air Liquid, has been carrying out a pilot project in Cofrentes Nuclear Power Plant focusing on the use of CO{sub 2} as an acidifier of the main condenser cooling system. (Author)

  7. Theory of gastric CO2 ventilation and its control during respiratory acidosis: implications for central chemosensitivity, pH regulation, and diseases causing chronic CO2 retention.

    Science.gov (United States)

    Dean, Jay B

    2011-02-15

    The theory of gastric CO(2) ventilation describes a previously unrecognized reflex mechanism controlled by neurons in the caudal solitary complex (cSC) for non-alveolar elimination of systemic CO(2) during respiratory acidosis. Neurons in the cSC, which is a site of CO(2) chemosensitivity for cardiorespiratory control, also control various gastroesophageal reflexes that remove CO(2) from blood. CO(2) is consumed in the production of gastric acid and bicarbonate in the gastric epithelium and then reconstituted as CO(2) in the stomach lumen from the reaction between H(+) and HCO(3)(-). Respiratory acidosis and gastric CO(2) distension induce cSC/vagovagal mediated transient relaxations of the lower esophageal sphincter to vent gastric CO(2) upwards by bulk flow along an abdominal-to-esophageal (=intrapleural) pressure gradient the magnitude of which increases during abdominal (gastric) compression caused by increased contractions of respiratory muscles. Esophageal distension induces cSC/nucleus ambiguus/vagovagal reflex relaxation of the upper esophageal sphincter and CO(2) is vented into the pharynx and mixed with pulmonary gas during expiration or, alternatively, during eructation. It is proposed that gastric CO(2) ventilation provides explanations for (1) the postprandial increase in expired CO(2) and (2) the negative P(blood - expired)CO₂difference that occurs with increased inspired CO(2). Furthermore, it is postulated that gastric CO(2) ventilation and alveolar CO(2) ventilation are coordinated under dual control by CO(2) chemosensitive neurons in the cSC. This new theory, therefore, presupposes a level of neural control and coordination between two previously presumed dissimilar organ systems and supports the notion that different sites of CO(2) chemosensitivity address different aspects of whole body pH regulation. Consequently, not all sites of central chemosensitivity are equal regarding the mechanism(s) activated for CO(2) elimination. A distributed CO(2) chemosensitive network-at least nine different areas in the CNS, including the cSC, have been reported to date-may reflect the complexity and dynamic nature of the fundamental neural circuitry required to achieve CO(2)/pH regulation across multiple organ systems under various states of arousal, oxygenation, pH status, and redox state. Moreover, coordination of respiratory and digestive control networks through the cSC could also account for the frequent co-expression of pulmonary diseases that cause chronic respiratory acidosis (and overstimulation of cSC neurons) with peptic ulcer disease or gastroesophageal reflux disease. Copyright © 2010 Elsevier B.V. All rights reserved.

  8. Karakteristik Enzim Digesti, Protease dan Amilase, Ikan Gurami (Osphronemus gouramy Lac. pada Fase Pertumbuhan

    Directory of Open Access Journals (Sweden)

    Untung Susilo

    2015-05-01

    Full Text Available Suatu penelitian untuk mengetahui karakteristik enzim digesti, protease dan amilase pada ikan gurami, Osphronemus gouramy Lac., telah dilakukan dengan metode survey. Jumlah ikan yang digunakan untuk penelitian sebanyak 25 ekor yang dikelompokan menjadi tiga kelompok ukuran13,29, 35,86 dan 91,86 g/ekor. Hasil penelitian menunjukkan bahwa aktivitas protease digesti ikan gurami berbeda secara signifikan pada segmen usus dan pH buffer yang berbeda (P.05, namun berbeda secara signifikan diantara ukuran ikan yang berbeda (P<.05, dan aktivitas amilase tertinggi dijumpai pada ikan dengan ukuran terkecil. Kesimpulan yang dapat diambil adalah bahwa aktivitas protease dan amilase dijumpai sepanjang saluran digestinya baik pada ikan yang berukuran kecil maupun besar. Aktivitas protease umumnya tinggi pada suasana asam dan netral pada usus depan dan tengah. Aktivitas protease ikan yang berukuran besar lebih rendah dari pada ikan yang berukuran lebih kecil. Aktivitas amilase tidak terdapat perbedaan diantara segmen usus yang diuji, namun aktivitas amilase tertinggi dijumpai pada ikan dengan berat rata-rata 13,29 g/ekor

  9. Neuronal pH regulation

    DEFF Research Database (Denmark)

    Vorstrup, S; Jensen, K E; Thomsen, C

    1989-01-01

    The intracellular pH in the brain was studied in six healthy volunteers before and immediately after the administration of 2 g of acetazolamide. Phosphorus-31 nuclear magnetic resonance spectroscopy by a 1.5 tesla whole-body scanner was used. The chemical shift between the inorganic phosphate...

  10. Relative contribution of ruminal buffering systems to pH regulation in feedlot cattle fed either low- or high-forage diets.

    Science.gov (United States)

    Chibisa, G E; Beauchemin, K A; Penner, G B

    2016-07-01

    The relative contribution of ruminal short-chain fatty acid (SCFA) absorption and salivary buffering to pH regulation could potentially change under different dietary conditions. Therefore, the objective of this study was to investigate the effects of altering the ruminal supply of rapidly fermentable carbohydrate (CHO) on absorptive function and salivation in beef cattle. Eight heifers (mean BW±SD=410±14 kg) were randomly allocated to two treatments in a crossover design with 37-day periods. Dietary treatments were barley silage at 30% low forage (LF) or 70% high forage (HF) of dietary dry matter (DM), with the remainder of the diet consisting of barley grain (65% or 25% on a DM basis) and a constant level (5%) of supplement. The LF and HF diets contained 45.3% and 30.9% starch, and 4.1% and 14.0% physically effective fiber (DM basis), respectively. Ruminal pH was continuously measured from day 17 to day 23, whereas ruminal fluid was collected on day 23 to determine SCFA concentration. Ruminal liquid passage rate was determined on day 23 using Cr-ethylenediaminetetraacetic acid. Eating or resting salivation was measured by collecting masticate (days 28 and 29) or saliva samples (days 30 and 31) at the cardia, respectively. On days 30 and 31, the temporarily isolated and washed reticulo-rumen technique was used to measure total, and Cl--competitive (an indirect measure of protein-mediated transport) absorption of acetate, propionate and butyrate. As a result of the higher dietary starch content and DM intake, the ruminal supply of rapidly fermentable CHO, total ruminal SCFA concentration (118 v. 95 mM; Pruminal pH in cattle with greater rapidly fermentable CHO intake was attributed to an increase in SCFA production and decrease in salivation, which were not compensated for by an increase in epithelial permeability.

  11. Efecto in vitro del D-003 sobre la actividad enzimática de la 5-lipoxigenasa (5-LOX

    Directory of Open Access Journals (Sweden)

    Yohani Pérez Guerra

    Full Text Available Introducción: el D-003, mezcla de ácidos alifáticos primarios superiores purificada de la cera de la caña, inhibe la síntesis de colesterol. Trabajos recientes han demostrado que el D-003 resulta efectivo en modelos experimentales de osteoartritis y que inhibe la actividad de las enzimas COX1 y COX2, preferentemente la COX1, sin producir gastrotoxicidad. Ha sido referido que los inhibidores duales de las enzimas COX y 5-LOX presentan efectos antinflamatorios desprovistos de gastrotoxicidad o que incluso, pueden resultar gastroprotectores. De acuerdo con estos antecedentes, el D-003 podría ser un inhibidor dual de dichas enzimas. Objetivo: investigar el efecto in vitro del D-003 sobre la actividad enzimática de la 5-LOX, utilizando la fracción citosólica de leucocitos polimorfonucleares de ratas. Métodos: se utilizaron las condiciones de ensayo siguientes: fracción citosólica (50 µg de proteína disuelta en solución reguladora borato 0,2 mol/L (pH 9 y ácido linoleico (7,8-250 mmol/L como sustrato, ensayándose muestras paralelas incubadas con Tween-20/H2O (2 % (vehículo, D-003 (0,6-6 000 µg/mL o extracto de Perna canaliculus (50 µg/mL (sustancia de referencia. Se evaluó la actividad enzimática mediante el cambio de absorbancia a 234 nm producido por la formación de dienos conjugados y medido en espectrofotómetro UV-visible. Resultados: la adición de D-003 produjo una inhibición dosis dependiente de la actividad enzimática de la 5-LOX (r= 0,975; p< 0,05 (CI50= 23,06 µg/mL in vitro. La magnitud de esta inhibición fue moderada, ya que la inhibición máxima, alcanzada a partir de 1 250 µg/mL, resultó de solo un 30 %. Conclusiones: el estudio demuestra que el D-003 es capaz de inhibir la actividad enzimática de la 5-LOX in vitro, pero moderadamente.

  12. Desempenho de frangos de corte e digestibilidade de rações com sorgo ou milheto e complexo enzimático

    Directory of Open Access Journals (Sweden)

    Paulo Ricardo de Sá da Costa Leite

    2011-03-01

    Full Text Available O objetivo deste trabalho foi avaliar a digestibilidade de rações formuladas com sorgo (Sorghum bicolor, ou milheto (Pennisetum glaucum e adição de um complexo enzimático comercial composto de amilase, carboidrases, proteases e fitase, e o desempenho de frangos de corte. Para avaliação de desempenho, foram alojados 1.800 pintos de corte machos em delineamento em blocos ao acaso, em arranjo fatorial 2x2, com quatro tratamentos (rações com sorgo ou milheto com ou sem adição de complexo enzimático e cinco repetições com 60 aves por unidade experimental. Nos ensaios de metabolismo realizados dos 10 aos 14 e dos 24 aos 28 dias, foram distribuídos 420 pintos machos em delineamento inteiramente casualizado, em esquema fatorial 2x2 (rações com milheto ou sorgo com ou sem adição de complexo enzimático. Os dados foram submetidos à análise de variância e as médias comparadas com o teste F para contrastes ortogonais. Nas rações elaboradas com sorgo, a adição do complexo enzimático proporcionou melhores coeficientes de digestibilidade da gordura e do nitrogênio. A adição de complexo enzimático em rações formuladas com sorgo melhora a conversão alimentar somente na fase inicial de criação dos frangos, enquanto a adição de complexo enzimático não melhora o desempenho de frango nas rações elaboradas com milheto.

  13. Síntesis y análisis estructural de glicomiméticos como potenciales inhibidores enzimáticos

    OpenAIRE

    Témpera, Carlos Agustín

    2013-01-01

    Como objetivo general, se propuso desarrollar Glicomiméticos que puedan ser empleados en el diseño de inhibidores enzimáticos. Como objetivos particulares se propuso: Sintetizar pseudos N-disacáridos unidos mediante un grupo sulfonamido ó metilén bissulfonamido Realizar el análisis estructural de los compuestos obtenidos, empleando principalmente Resonancia Magnética Nuclear Estudiar la actividad biológica de los compuestos sintetizados como Inhibidores Enzimáticos. Facultad de Ciencias Ex...

  14. Actividad enzimática del suelo -Deshidrogenasa , -ß Glucosidasa , Fosfatasa y Ureasa - bajo diferentes cultivos.

    Directory of Open Access Journals (Sweden)

    Carlos Henríquez

    2014-01-01

    Full Text Available Se analizó la actividad de 4 enzimas del suelo en fincas bajo diferentes manejos agronómi - cos y en diferentes tipos de suelos. Las enzimas evaluadas fueron la Fosfatasa, β-Glucosidasa, Deshidrogenasa y Ureasa. Se tomaron muestras de suelo de los primeros 20 cm de profundidad con el fin de analizarlas químicamente y determinar la actividad de las 4 enzimas a evaluar. Se encontró que la actividad de la Deshidrogenasa varió de 0,13 a 4,46 con un promedio de 1,17 (μg INTF.g - 1 .h -1 ; la β-Glucosidasa varió de 31,9 a 208,1 con un promedio de 108,1 (μg PNP.g -1 .h -1 . Por otro lado la Fosfatasa tuvo valores entre 413,4 y 3043,6 con un promedio de 1521,5 μg PNP.g -1 .h -1 , mientras que la Ureasa varió de 12,5 a 52,8 con un promedio de 38,3 μg N-NH4.g -1 .h -1 . La Fos - fatasa correlacionó con el porcentaje de materia orgánica y de carbono en el suelo (0,75 y 0,76 respectivamente, p<0,01; se encontró una rela - ción inversa entre la actividad de esta enzima y el contenido de P disponible (r de -0,53, p<0,10. La Ureasa correlacionó con la Fosfatasa (r de 0,61 p<0,05 y con la Deshidrogenasa (r de -0,77 p<0,01. El análisis multivariado de conglomera - dos a partir de la actividad enzimática permitió hacer grupos similares según el tipo de cultivo, lo que evidencia el potencial de utilización que tiene esta propiedad para realizar estudios más detallados.

  15. Sistema enzimático antisenescencia, catalasa-superóxido dismutasa, de frutos de pitahaya (Hylocereus undatus almacenados con frío

    Directory of Open Access Journals (Sweden)

    R. Balois-Morales

    2008-01-01

    Full Text Available El objetivo de este estudio fue evaluar efecto del frío en el sistema enzimático antisenescencia catalasa (EC. 1.11.1.6; CAT- superóxido dismutasa (EC. 1.15.1.1; SOD de frutos de pitahaya (Hylocereus undatus durante el almacenamiento con frío y temperatura ambiente. Lotes de frutos, cosechados en Tehuacán, Puebla, fueron almacenados cuatro días a 22±1 °C, y a 3, 7 y 11±1 °C durante 7, 14 y 21 días, y cuatro días a 22±1 °C al concluir cada período de frigoalmacenamiento. Se determinó la actividad CAT y SOD en la pulpa de los frutos de 20 tratamientos que incluyó un testigo sin almacenamiento. Cuatro días de almacenamiento a 22±1 °C no modificaron la actividad CAT o SOD (18.75 y 3.25 U·g-1, respectivamente, respecto al testigo. El almacenamiento con frío disminuyó significativamente la actividad CAT, respecto al testigo. La inhibición de la actividad CAT no mostró una tendencia dependiente de la temperatura o tiempo de almacenamiento con frío; pero la inhibición media en los frutos almacenados a 11±1 °C (13.07 U·g-1 fue ligeramente menor a la alcanzada con el almacenamiento a 3 y 7±1 °C (12.39 U·g-1. La inhibición de la actividad CAT fue revertida parcial o totalmente con el almacenamiento a 22±1 °C por cuatro días después del frigoalmacenamiento. La actividad SOD no se afectó significativamente con el frío y, en general, fue incrementada con el almacenamiento a 22±1 °C, complementario al frigoalmacenamiento. En respuesta al almacenamiento con frío, la actividad CAT es inhibida, pero SOD, que apoya la disminución de los síntomas de senescencia, generados por el estrés oxidativo, se activa en los frutos de pitahaya.

  16. Efecto in vitro del D-002 sobre la actividad enzimática de la 5-lipoxigenasa (5-LOX

    Directory of Open Access Journals (Sweden)

    Yohani Pérez Guerra

    2012-06-01

    Full Text Available Introducción: el D-002, mezcla de seis alcoholes alifáticos primarios de alto peso molecular purificada de la cera de abejas, ha mostrado efectos antiinflamatorios, sin gastrotoxicidad secundaria, enmodelos experimentales. El tratamiento oral con D-002 reduce las concentraciones de leucotrieno B4 (LTB4 en exudados pleurales de ratas con pleuresía por carragenina, sugiriendo que podría inhibir la actividad enzimática de la 5-lipooxigenasa (5-LOX, si bien los mecanismos de la acción antiinflamatoria del D-002 no habían sido aún explorados. Objetivo: evaluar el efecto in vitro del D-002 sobre la actividad de la 5-LOX, utilizando la fracción citosólica de homogenatos de hígado de ratas. Métodos: las condiciones de ensayo utilizadas fueron las siguientes: fracción citosólica (50 µg de proteína disuelta en solución reguladora borato 0,2 mol/L (pH 9 y ácido linoleico (7,8-250 mmol/L como sustrato, ensayándose muestras paralelas incubadas con Tween-20/H2O (2 % (vehículo, muestras controles, D-002 (0,9-1 000 µg/mL o Lyprinol (500 µg/mL (sustancia de referencia. La actividad enzimática se evaluó mediante el cambio de absorbancia a 234 nm producido por la formación de dienos conjugados y medido en espectrofotómetro UV-visible. Resultados: la adición in vitro del D-002 produjo una inhibición significativa, dependiente de la dosis (r= 0,980; p< 0,001 (IC50= 95,34 µg/mL e incompetitiva de la actividad de la 5-LOX, cuya inhibición máxima (70 % se alcanzó con 500 µg/mL. Conclusiones: con este estudio se demuestra que el D-002 es capaz de inhibir la actividad enzimática de la 5-LOX, efecto que podría explicar, al menos parcialmente, su acción antiinflamatoria en modelos experimentales in vivo.

  17. Atmosphere and Ambient Space

    DEFF Research Database (Denmark)

    Schmidt, Ulrik

    Atmosphere and Ambient Space This paper explores the relation between atmosphere and ambient space. Atmosphere and ambient space share many salient properties. They are both ontologically indeterminate, constantly varying and formally diffuse and they are both experienced as a subtle, non......-signifying property of a given space. But from a certain point of view, the two concepts also designate quite dissimilar experiences of space. To be ’ambient’ means to surround. Accordingly, ambient space is that space, which surrounds something or somebody. (Gibson 1987: 65) Since space is essentially...... of a surrounding character, all space can thus be described as having a fundamentally ambient character. So what precisely is an ambient space, then? As I will argue in my presentation, ambient space is a sensory effect of spatiality when a space is experienced as being particularly surrounding: a ‘space effect...

  18. Influencia del uso de complejos enzimáticos en la segunda centrifugación de pastas de aceitunas

    Directory of Open Access Journals (Sweden)

    Jackisch, Björn Oliver

    2006-09-01

    Full Text Available Virgin olive oil is a natural fruit juice that conserves the flavor, aroma, vitamins and all the properties of the fruit from which it comes, being, in addition, the only vegetal oil that can be consumed as “virgin” or completely unrefined. The extraction of the juice from the olive is carried out exclusively by mechanical means in the first centrifugation step, in order to obtain directly consumable virgin olive oil. The use of enzymatic complexes in the process of a second centrifugation step has been contemplated in order to obtain a greater yield of refinable virgin oil.With this work, we attempt to demonstrate an increase in the yield of oil extraction with the use of an enzymatic complex in the second centrifugation and to confirm that its use does not affect the quality of the oils obtainedEl aceite de Oliva Virgen es un zumo de fruta natural que conserva el paladar, perfume, vitaminas y todas las propiedades del fruto del que procede, siendo, además, el único aceite vegetal que puede consumirse directamente virgen y crudo. Se defiende la elaboración del zumo de aceituna por medios exclusivamente mecánicos en la primera centrifugación, para obtener aceites de oliva vírgenes directamente consumibles, pero se contempla el uso de complejos enzimáticos en los procesos de segunda centrifugación, para incrementar los rendimientos en la obtención de aceites vírgenes refinables.Pretendemos demostrar con este trabajo que el uso de un complejo enzimático en la segunda centrifugación aumenta el rendimiento del aceite y, al mismo tiempo, confirmar que su uso no tiene efecto en los parámetros de calidad de los aceites obtenidos

  19. Altıntop Dilim Konservesi Üretiminde Enzim Kullanımı: I. Kabuk Soyma

    Directory of Open Access Journals (Sweden)

    Osman Kola

    2015-02-01

    Full Text Available Bu çalışmada; altıntop dilim konservesi yapımında kabuk soyma ve dilim zarının uzaklaştırılması amaçlarıyla enzim çözeltisi kullanılmasının etkileri araştırılmıştır. Bunun için: önce, çözeltinin meyve kabuğu içine nüfuz ettirilmesi amacıyla uygulanabilecek uygun işlemler, sonra da, enzim çözeltisi uygulamasının kabuk soyma işlemleri üzerindeki etkileri araştırılmıştır. Kabuk soyma işleminde meyvenin; 95 °C sıcaklıktaki suda 5 dakika bekletildikten sonra kapak açma ve kabuk çizme işlemlerini takiben, su ya da %0.5 düzeyindeki Peelzym II çözeltisi içine konulan meyvelerin 1.76 kg/cm2 (25 psi basınç altında 60 saniye süreyle işleme tabi tutulmasının, hem işlemin etkinliği hem de son ürünün kalitesi açısından uygun olabileceği sonucuna varılmıştır.

  20. Producció enantioselectiva de diols vicinals i αhidroxicetones amb oxidoreductases de llevat. Aplicació de tècniques d’autoclonació per la generación d’enzims i soques modificades

    OpenAIRE

    Calam Cervera, Eduard

    2015-01-01

    Les transformacions catalitzades per enzims s'utilitzen cada cop més per a la síntesis d'intermediaris útils destinats a les indústries farmacèutiques, agroalimentàries i de química fina. La utilització d'enzims en síntesi química compleix amb els principis de la "química verda", com per exemple en la utilització de solvents més segurs (les reaccions enzimàtiques són realitzades usualment, en medi aquós), en el disseny de reaccions amb millor eficiència energètica (les reaccions enzimàtiques ...

  1. The future is 'ambient'

    Science.gov (United States)

    Lugmayr, Artur

    2006-02-01

    The research field of ambient media starts to spread rapidly and first applications for consumer homes are on the way. Ambient media is the logical continuation of research around media. Media has been evolving from old media (e.g. print media), to integrated presentation in one form (multimedia - or new media), to generating a synthetic world (virtual reality), to the natural environment is the user-interface (ambient media), and will be evolving towards real/synthetic undistinguishable media (bio-media or bio-multimedia). After the IT bubble was bursting, multimedia was lacking a vision of potential future scenarios and applications. Within this research paper the potentials, applications, and market available solutions of mobile ambient multimedia are studied. The different features of ambient mobile multimedia are manifold and include wearable computers, adaptive software, context awareness, ubiquitous computers, middleware, and wireless networks. The paper especially focuses on algorithms and methods that can be utilized to realize modern mobile ambient systems.

  2. Enzimas de função hepática na aflatoxicose aguda experimental em frangos de corte Hepatic enzimes function in experimental acute enzimes aflatoxicosis in broilers

    Directory of Open Access Journals (Sweden)

    Adriana Borsa

    1998-12-01

    Full Text Available Com o objetivo de avaliar a função hepática de aves experimentalmente intoxicadas por aflatoxina, com e sem uso de bentonita sódica, foram utilizados 40 (quarenta frangos de corte, machos, linhagem Ross, divididos em 4 (quatro grupos de 10 (dez. animais, sendo que cada grupo foi submetido a um tratamento: T1 - controle (ração sem aflatoxina ou bentonita, T2 - ração com 5ppm de aflatoxina, T3 - ração com 5ppm de aflatoxina e 0,5% de bentonita sódica e T4 - ração com 0,5% de bentonita sódica. Todos estes tratamentos foram aplicados do 1° ao 42 ° dia de vida das aves. Aos 21, 35 e 42 dias de idade, foram analisados os níveis séricos das enzimas aspartato aminotransferase (AST, alanina aminotransferase (ALT, lactato desidrogenase (LDH e gama glutamiltransferase (GGT, A análise da variância mostrou que houve interação entre os tratamentos e datas de colheita de material, para as seguintes variáveis: AST, LDH e GGT. Para estas, foi aplicado o teste de Tukey, comparando-se as médias de cada tratamento em cada data. Observou-se que as enzimas AST, ALT e GGT não apresentaram diferenças significativas entre tratamentos, porém, nos tratamentos l e 2, a AST apresentou um aumento linear (pThe aim ofthis study is to evaluate ïhe hepatic function of experimentally intoxicated broilers by aflatoxin with and without sodium bentonite. Forty Ross mole broilers, were used divided into 4 groups of 10 birds, and such groups have been submitted to the following treatments: T1- control (feed without aflatoxin or sodium bentonite, T2- feed containing 5ppm of aflatoxin, T3- feed containing 5ppm of aflatoxin and 0.5% of sodium bentonite and T4- feed containing 0.5% of sodium bentonite. Ali these treatments have been appiied from the l st to the 42nd day of lif e. On the days 21, 35 and 42, the serum leveis of the enzimes aspartate aminotransferase (AST, alanino aminotransferase (ALT, lactate dehydrogenase (LDH and gamma glutamiltransferase (GGT

  3. Microbiota intestinal e desempenho de frangos alimentados com rações elaboradas com sorgo ou milheto e complexo enzimático

    Directory of Open Access Journals (Sweden)

    P.R.S.C. Leite

    2012-12-01

    Full Text Available Avaliou-se a suplementação enzimática em rações contendo milheto ou sorgo sobre a microbiota intestinal e o desempenho de frangos. Foram alojados 420 pintos - ensaio de microbiota - e 1.200 pintos - ensaio de desempenho -, submetidos aos tratamentos com sorgo ou milheto suplementados ou não com complexo enzimático. O delineamento utilizado foi inteiramente ao acaso, com sete repetições de 15 aves cada, para o ensaio de microbiota, e em blocos ao acaso, com cinco repetições e 60 aves por parcela para desempenho. Foram realizadas pesquisas de bactérias Gram-negativas e contagem do número total de microrganismos aeróbios do intestino delgado. A suplementação enzimática não afetou a microbiota intestinal de frangos aos 14 e 28 dias de idade. Houve efeito da suplementação enzimática nas dietas com sorgo para conversão alimentar na fase pré-inicial. Nas dietas com milheto, a suplementação melhorou o ganho de peso no período de um a 35 dias de idade. No período total de criação, de um a 42 dias, não foi observado efeito da suplementação para milheto ou sorgo. Conclui-se que a utilização da suplementação de enzimas em rações com sorgo ou milheto pode melhorar os resultados de desempenho, dependendo da fase de crescimento de frangos de corte.

  4. Níveis decrescentes de proteína em dietas suplementadas com complexo enzimático para coelhos em crescimento. 1. Desempenho produtivo

    Directory of Open Access Journals (Sweden)

    Dias J.C.C.A.

    2000-01-01

    Full Text Available Estudou-se o efeito da redução do nível protéico da dieta, com e sem suplementação enzimática, sobre o desempenho produtivo de coelhos em crescimento. Em um delineamento inteiramente ao acaso, utilizaram-se 54 coelhos da raça Nova Zelândia Branco, de ambos os sexos, desmamados aos 35 dias de idade e divididos em cinco grupos (tratamentos, quatro de 11 e um de 10 coelhos. Os tratamentos constituíram-se de dietas calculadas como isoenergéticas e isoaminoacídicas com 17, 16, 15 e 14% de proteína bruta (PB, suplementadas com 0,05% de um complexo enzimático comercial (VegproÒ , com atividade de protease e de celulase, e uma dieta controle com 17% de PB sem adição de complexo enzimático. Os animais submetidos aos tratamentos com 14 e 15% PB apresentaram melhores resultados no ensaio de crescimento, observando-se influência significativa (P<0,05 do teor de PB sobre o peso vivo final, peso vivo final metabólico, ganho de peso diário, consumo diário de dieta, consumo diário de proteína bruta e conversão alimentar. A redução do nível de proteína bruta da dieta para 14% com suplementação enzimática e corrigida quanto aos aminoácidos mais limitantes para os teores recomendados pela literatura não influenciou negativamente as características estudadas.

  5. Licenciamento ambiental e sustentabilidade

    Directory of Open Access Journals (Sweden)

    Marcelo Macedo Valinhas

    2011-12-01

    Full Text Available A sustentabilidade está apoiada principalmente nas dimensões econômica, ambiental e social. No entanto, sem a dimensão política ela não se constrói. Um dos principais instrumentos de comando e controle da política nacional de meio ambiente, o licenciamento ambiental é um processo contínuo de gestão ambiental pública e privada. Analisou-se o processo de licenciamento ambiental como acoplamento estrutural entre os sistemas social, econômico e ambiental. Apesar da constatação de críticas aos mecanismos de comando e controle dos últimos anos, foi verificado que o Estado do Rio de Janeiro tem buscado integrar a política ambiental do Estado à gestão ambiental privada e que esta integração busca atender às demandas dos sistemas sociais e econômicos para as questões ambientais. Em linhas gerais, este caminho segue as estratégias e ações propostas na Agenda 21 brasileira.

  6. Algorithms in ambient intelligence

    NARCIS (Netherlands)

    Aarts, E.H.L.; Korst, J.H.M.; Verhaegh, W.F.J.; Weber, W.; Rabaey, J.M.; Aarts, E.

    2005-01-01

    We briefly review the concept of ambient intelligence and discuss its relation with the domain of intelligent algorithms. By means of four examples of ambient intelligent systems, we argue that new computing methods and quantification measures are needed to bridge the gap between the class of

  7. Persuasion in Ambient Intelligence

    NARCIS (Netherlands)

    Kaptein, M.C.; Markopoulos, P.; Ruyter, de B.E.R.; Aarts, E.H.L.

    2010-01-01

    Although the field of persuasive technologies has lately attracted a lot of attention, only recently the notion of ambient persuasive technologies was introduced. Ambient persuasive technologies can be integrated into every aspect of life, and as such have greater persuasive power than the

  8. Procesado acuoso de soja con tecnología enzimática: extracción de aceite y producción de aislados

    Directory of Open Access Journals (Sweden)

    Domínguez, H.

    1995-02-01

    Full Text Available An enzymatic treatment was incorporated before the alkaline extraction during the aqueous processing of soya beans for oil and protein. The degradation on the plant cell wall structure by the enzymatic action, enhances the oil extractability by 10% of the potential extractable oil. No significant effect on the protein extractability was detected, although this effect was probably masked by the alkaline extraction. After the aqueous extraction, 30% of active enzyme was eliminated in the whey. This can be recovered and reutilized in the next stage.

    Se ha incorporado una etapa de tratamiento enzimático previa a la extracción acuosa en condiciones alcalinas durante el procesado para obtención de aceite y proteína de soja. La degradación de la estructura de la pared vegetal, causada por el ataque enzimático, aumenta la extractabilidad del aceite en un 10% respecto al aceite total extraíble. No se apreció efecto sobre el rendimiento de proteína debido posiblemente a que la posterior extracción a pH 9 inhibe los efectos de la acción enzimática. Tras la realización del proceso de extracción acuoso, se elimina en el suero un 30% de enzima activa, que puede ser reutilizada en el proceso.

  9. PENGARUH SUPLEMENTASI RAGI TAPE DAN ENZIM KOMPLEKS DALAM RANSUM YANG MENGANDUNG POD KAKAO TERHADAP PENAMPILAN ITIK BALI UMUR 2-8 MINGGU

    Directory of Open Access Journals (Sweden)

    IDA BAGUS GAGA PARTAMA

    2014-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi enzim kompleks dan ragi tape dalam ransumyang mengandung pod kakao terhadap penampilan itik bali jantan umur 2-8 minggu, dilakukan di Denpasar,Bali. Rancangan yang dipergunakan dalam penelitian ini adalah rancangan acak lengkap (RAL dengan empatmacam perlakuan dan enam kali ulangan. Tiap ulangan menggunakan 5 ekor itik Bali jantan umur 2 minggu denganberat badan homogen (243±8,05 g. Ke empat perlakuan yang dicobakan, yaitu itik yang diberi ransum basaltanpa penggunaan pod kakao sebagai kontrol (A, ransum dengan 15% tepung pod kakao (B, ransum dengan 15%pod kakao+0,20% ragi tape (C, dan ransum dengan 15% pod kakao+0,20% enzim kompleks (D. Ransum yangdiberikan selama periode penelitian (umur 2-8 minggu disusun dengan kandungan protein kasar 16% dan energitermetabolis 2900 kkal/kg. Ransum dan air minum diberikan ad libitum. Variabel yang diamati adalah: konsumsiransum, air minum, berat badan akhir, pertambahan berat badan, dan feed conversion ratio (FCR. Hasil penelitianmenunjukkan bahwa penggunaan 15% pod kakao (B dalam ransum secara nyata (P0,05 dengankontrol (A. Dari hasil penelitian ini dapat disimpulkan bahwa penggunaan 15% pod kakao dalam ransum itik Balijantan umur 2-8 minggu belum dapat direkomendasikan, dan baru dapat direkomendasikan apabila disuplementasidengan 0,20% ragi tape atau enzim kompleks.

  10. HUBUNGAN DOSIS TEPUNG GEMBILI (Dioscorea esculenta DENGAN TINGKAT EKSPRESI ENZIM Ampk-α2 PADA MODEL TIKUS DIABETES MELITUS

    Directory of Open Access Journals (Sweden)

    neni oktiyani

    2015-06-01

    Full Text Available Abstract: Alternative methods of controlling glucose levels in patients with diabetes is by type of food, either by utilizing yam flour. At the flour contained inulin and resistant starch that can activate the enzyme AMPK-α2. Activation of these enzymes will stimulate glucose transport in skeletal muscle and liver, thus causing a decrease in glucose production. Varying doses of flour is expected to affect the expression of AMPK-α2. This study aims to dosage relationship yam flour (Dioscorea esculenta with tigkat-α2 AMPK enzyme expression in the nucleus skeletal muscle and liver in mouse models of diabetes mellitus. The study was a quasi-experimental design with Post Test Only Group Design. Rats were divided into 5 (five groups, healthy mice, the mice with type 2 diabetes, and type 2 groups of diabetic rats with dietary intake yam flour addition of 1.25 g (TG-1:25,, 2.5 g (TG -2.5, 5.0 g (TG-5.0. Yam flour is mixed into the rat diet feed with varying doses. The results showed no significant correlation between the dose of yam flour with AMPK-α2 expression levels in skeletal muscle nuclei (p = 0.312 and liver (p = 0.474 in a mouse model of DM. The need for other studies using other types of food as an alternative arrangement of food for patients with diabetes Keywords: diabetes mellitus type 2, AMPK-α2, yam flour Abstrak: Metode alternatif pengendalian kadar glukosa pada penderita DM adalah dengan pengaturan jenis makanan, salah satunya dengan memanfaatkan tepung gembili. Pada tepung terdapat inulin dan pati resisten yang dapat mengaktivasi enzim Ampk-α2. Aktivasi enzim ini akan menstimulasi transpor glukosa di otot skelet dan hepar, sehingga menyebabkan terjadinya penurunan produksi glukosa. Dosis bervariasi dari tepung diharapkan mampu berpengaruh terhadap ekspresi Ampk-α2. Penelitian ini bertujuan untuk hubungan dosis tepung gembili (Dioscorea esculenta dengan tigkat ekspresi enzim Ampk-α2 di nukleus otot skelet dan hepar pada model

  11. Acoustic ambient noise recorder

    Digital Repository Service at National Institute of Oceanography (India)

    Saran, A.K.; Navelkar, G.S.; Almeida, A.M.; More, S.R.; Chodankar, P.V.; Murty, C.S.

    with a robust outfit that can withstand high pressures and chemically corrosion resistant materials. Keeping these considerations in view, a CMOS micro-controller-based marine acoustic ambient noise recorder has been developed with a real time clock...

  12. Algorithms in ambient intelligence

    NARCIS (Netherlands)

    Aarts, E.H.L.; Korst, J.H.M.; Verhaegh, W.F.J.; Verhaegh, W.F.J.; Aarts, E.H.L.; Korst, J.H.M.

    2004-01-01

    In this chapter, we discuss the new paradigm for user-centered computing known as ambient intelligence and its relation with methods and techniques from the field of computational intelligence, including problem solving, machine learning, and expert systems.

  13. Ambient Dried Aerogels

    Science.gov (United States)

    Jones, Steven M.; Paik, Jong-Ah

    2013-01-01

    A method has been developed for creating aerogel using normal pressure and ambient temperatures. All spacecraft, satellites, and landers require the use of thermal insulation due to the extreme environments encountered in space and on extraterrestrial bodies. Ambient dried aerogels introduce the possibility of using aerogel as thermal insulation in a wide variety of instances where supercritically dried aerogels cannot be used. More specifically, thermoelectric devices can use ambient dried aerogel, where the advantages are in situ production using the cast-in ability of an aerogel. Previously, aerogels required supercritical conditions (high temperature and high pressure) to be dried. Ambient dried aerogels can be dried at room temperature and pressure. This allows many materials, such as plastics and certain metal alloys that cannot survive supercritical conditions, to be directly immersed in liquid aerogel precursor and then encapsulated in the final, dried aerogel. Additionally, the metalized Mylar films that could not survive the previous methods of making aerogels can survive the ambient drying technique, thus making multilayer insulation (MLI) materials possible. This results in lighter insulation material as well. Because this innovation does not require high-temperature or high-pressure drying, ambient dried aerogels are much less expensive to produce. The equipment needed to conduct supercritical drying costs many tens of thousands of dollars, and has associated running expenses for power, pressurized gasses, and maintenance. The ambient drying process also expands the size of the pieces of aerogel that can be made because a high-temperature, high-pressure system typically has internal dimensions of up to 30 cm in diameter and 60 cm in height. In the case of this innovation, the only limitation on the size of the aerogels produced would be in the ability of the solvent in the wet gel to escape from the gel network.

  14. Data based ambient lighting control

    NARCIS (Netherlands)

    2012-01-01

    In controlling an ambient lighting element, a category of data being rendered by a host is identified, ambient lighting data associated with the identified category is retrieved, and the retrieved ambient lighting data is rendered in correspondence with the rendered data. The retrieved ambient

  15. Optimización de la producción de un extracto enzimático keratinásico a ser utilizado en la industria textil

    Directory of Open Access Journals (Sweden)

    Daniel Pippolo

    2011-04-01

    Full Text Available El Sector Textil Uruguayo tiene dificultades para alcanzar los mejores estándares de calidad requeridos por los mercados internacionales que le permitan competir con mayores posibilidades. Para esto, un procedimiento promisorio, y amigable con el medioambiente, es el tratamiento enzimático de la lana con el que se obtienen resultados de similares características al de los sistemas de oxidación, mayores rendimientos tintóreos  con un menor daño de las fibras, además de obtenerse efluentes sensiblemente menos contaminantes.Este trabajo optimizó la producción de enzimas keratinásicas a partir de un Bacillus sp., obtenido de un screening de cepas realizado por el Departamento de Bioingeniería de la Facultad de Ingeniería y estudió el comportamiento tintóreo de tops de lana tratada con los extractos enzimáticos obtenidos, respecto a la lana sin tratar. El estudio se llevó a cabo empleando la Metodología de Superficie de Respuesta. Se determinaron las condiciones de operación óptimas del fermentador de laboratorio de 3L, correspondientes a una agitación de 600 rpm y una aireación de 2,5 L/min, que se correlaciona con un  coeficiente volumétrico de transferencia de oxígeno del sistema de kLa de 140 h-1, que optimizaron la producción de enzimas keratinásicas (773±77 U/mL. Para la medida de la actividad keratinásica se desarrolló una técnica que utiliza keratin-azure como sustrato.El estudio del comportamiento tintóreo de la lana tratada con los extractos enzimáticos keratinásicos, respecto a la lana sin tratar, confirmó que las diferencias de color obtenidas con las lanas tratadas son superiores cuanto mayor es el valor de actividad keratinásica del extracto. Los tops fueron teñidos con el colorante  Rojo LANASOL CE. El valor máximo de la diferencia de color total medida en coordenadas Cielab, fue de 5,88. Se observó, que en todos los casos los colores obtenidos en los tops después de ser tratados enzim

  16. Ambient oxygen promotes tumorigenesis.

    Directory of Open Access Journals (Sweden)

    Ho Joong Sung

    2011-05-01

    Full Text Available Oxygen serves as an essential factor for oxidative stress, and it has been shown to be a mutagen in bacteria. While it is well established that ambient oxygen can also cause genomic instability in cultured mammalian cells, its effect on de novo tumorigenesis at the organismal level is unclear. Herein, by decreasing ambient oxygen exposure, we report a ∼50% increase in the median tumor-free survival time of p53-/- mice. In the thymus, reducing oxygen exposure decreased the levels of oxidative DNA damage and RAG recombinase, both of which are known to promote lymphomagenesis in p53-/- mice. Oxygen is further shown to be associated with genomic instability in two additional cancer models involving the APC tumor suppressor gene and chemical carcinogenesis. Together, these observations represent the first report directly testing the effect of ambient oxygen on de novo tumorigenesis and provide important physiologic evidence demonstrating its critical role in increasing genomic instability in vivo.

  17. Caracterização da agressividade e atividade enzimática de isolados de Colletotrichum spp. associados à antracnose do abacate

    Directory of Open Access Journals (Sweden)

    Hugo José Tozze Júnior

    Full Text Available RESUMO A antracnose é a principal doença pós-colheita do abacate. O presente trabalho teve como objetivos caracterizar a agressividade e a atividade enzimática de 30 isolados de Colletotrichum spp. de abacate. Abacates ‘Fuerte’ foram inoculados com disco de BDA contendo estruturas de cada isolado e após sete dias mensuraram-se os diâmetros das lesões. Dois grupos de isolados foram distinguidos quanto à agressividade pelo teste de teste de Scott-Knott (p=0,05, um com lesões entre 34,0 e 38,2 mm e outro com lesões entre 38,7 e 44,0 mm de diâmetro. Para caracterização enzimática, avaliaram-se as áreas dos halos formados em substratos específicos para detecção de amilase, celulase, lacase, lipase, pectato liase e protease e a atividade da enzima catalase. Os isolados não produziram amilase suficiente para formação de halo em meio de cultura. Todos os isolados produziram catalase, pectato liase e protease, enquanto 46% produziram lacase e 97% tiveram as atividades da celulase e lipase detectadas. Distinguiram-se dois grupos de isolados para as enzimas catalase e pectato liase, cinco grupos para a celulase, seis grupos para a lacase e sete grupos para as enzimas lipase e protease, pelo teste de teste de Scott-Knott (p=0,05. Não houve relação entre as atividades enzimáticas e a agressividade dos isolados.

  18. Ambient ionization mass spectrometry

    International Nuclear Information System (INIS)

    Lebedev, A T

    2015-01-01

    Ambient ionization mass spectrometry emerged as a new scientific discipline only about ten years ago. A considerable body of information has been reported since that time. Keeping the sensitivity, performance and informativity of classical mass spectrometry methods, the new approach made it possible to eliminate laborious sample preparation procedures and triggered the development of miniaturized instruments to work directly in the field. The review concerns the theoretical foundations and design of ambient ionization methods. Their advantages and drawbacks, as well as prospects for application in chemistry, biology, medicine, environmetal analysis, etc., are discussed. The bibliography includes 194 references

  19. Detecção e caracterização de complexos multi-enzimáticos em secretoma de fungos filamentosos

    OpenAIRE

    Silva, Adelson Joel da

    2013-01-01

    As enzimas que degradam parede celular vegetal produzidas por micro-organismos possuem aplicações biotecnológicas importantes, incluindo a produção de bioetanol. Algumas batérias anaeróbicas são capazes de produzir complexos multi-enzimáticos chamados de celulosomos, enquanto os fungos filamentosos normalmente secretam enzimas hidrolíticas individuais que atuam sinergisticamente no processo de degradação de polissacarídeo. Neste trabalho, nós mostramos que os fungos filamentosos Trichoderm...

  20. Potential Marine Fungi Hypocreaceae sp. as Agarase Enzyme to Hydrolyze Macroalgae Gelidium latifolium (Potensi Jamur Hypocreaceae sp. sebagai Enzim Agarase untuk menghidrolisis Makroalga Gelidium latifolium

    Directory of Open Access Journals (Sweden)

    Mujizat Kawaroe

    2015-03-01

    Full Text Available Agarase dapat mendegradasi agar ke oligosakarida dan memiliki banyak manfaat untuk makanan, kosmetik, dan lain-lain. Banyak spesies pendegradasi agar adalah organismelaut. Beberapa agarase telah diisolasi dari genera yang berbeda dari mikroorganisme yang ditemukan di air dan sedimen laut. Hypocreaceae sp. diisolasi dari air laut Pulau Pari, Kepulauan Seribu, Jakarta, Indonesia. Berdasarkan hasil identifikasi gen 16S rDNA dari 500 basis pasangan, isolat A10 memiliki 99% kesamaan dengan Hypocreaceae sp. Enzim agarase ekstraseluler dari Hypocreaceae sp. memiliki pH dan suhu optimum pada 8 TrisHCl (0,148 μ.mL-1 dan 50°C (0,182 μ.mL-1, masing-masing. Enzim Agarase dari Hypocreaceae sp. mencapai kondisi optimum pada aktivitas enzim tertinggi selama inkubasi dalam 24 jam (0,323 μ.mL-1. SDS page mengungkapkan bahwa ada dua band dari protein yang dihasilkan oleh agarase dari Hypocreaceae sp. yang berada di berat molekul 39 kDa dan 44 kDa dan hidrolisis Gelidium latifolium diperoleh 0,88% etanol. Kata kunci: enzim agarase, Hypocreaceae sp., hidrolisis, fungi, rDNA. Agarase can degradedagarto oligosaccharide and has a lot of benefits for food, cosmetics, and others. Many species of agar- degrader are marine-organism. Several agarases have been isolated from different genera of microorganisms found in seawater and marine sediments. Hypocreaceae sp. was isolated from sea water of Pari Islands, Seribu Islands, Jakarta, Indonesia. Based on the results of the 16S rDNA gene identification of 500 base pairs, A10 isolates had 99 % similarity toHypocreaceae sp. The extracellular agarase enzyme from Hypocreaceae sp. have optimum pH and temperature at 8 TrisHCl (0.148 µ.mL-1 and 50 °C (0.182 µ.mL-1, respectively. Agarase enzyme of Hypocreaceae sp. reach an optimum condition at the highest enzyme activity during incubation in 24 hours (0.323 µ.mL-1. SDS Page revealed that there are two bands of protein produced by agarase of Hypocreaceae sp. which are at

  1. Polimerización enzimática con lacasa : nuevos sistemas adhesivos para fabricación de tableros de madera

    OpenAIRE

    Ferreira De Gouveia, Susana Margarida Alves

    2014-01-01

    La península ibérica concentra la mayor producción a nivel mundial de ligninas Kraft de E. globulus, que habitualmente son quemadas para producción de energía, sin considerar otras posibles vías de revalorización de este residuo. En esta Tesis Doctoral se propone caracterizar ligninas Kraft procedentes de E. globulus y determinar los efectos de la modificación enzimática sobre su estructura. Este es el punto de partida para una valorización integral de ligninas de E. globulus mediante tecnolo...

  2. ¿Todo tiempo pasado fue mejor? Cómo ha incidido la tecnología enzimática en el bienestar de la humanidad

    Directory of Open Access Journals (Sweden)

    Sonia Amparo Ospina S.

    2011-01-01

    La tecnología enzimática tiene su origen en los comienzos de la humanidad, cuando el hombre elaboraba queso utilizando cuajo (el cual contiene la enzima renina, sin entender qué sucedía en este proceso, pero aprovechando igualmente esta molécula tan interesante, cuya aplicación ha traspasado generaciones, incrementándose sus posibilidades de uso a medida que ha aumentado el conocimiento en las áreas biotecnológicas.

  3. Evaluación del proceso de extracción de aceite de aguacate hass (Persea americana Mill) utilizando tratamiento enzimático

    OpenAIRE

    Buelvas Salgado, G. A; Patiño Gómez, J. H; Cano-Salazar, J. A

    2012-01-01

    Introducción. El aceite de aguacate es rico en vitaminas A, B, C y E, y está compuesto mayoritariamente por ácidos grasos monoinsaturados los cuales reducen de manera efectiva el nivel de colesterol en la sangre, ayudando en la prevención de enfermedades cardiovasculares. Objetivo. Evaluar el rendimiento de extracción de aceite de aguacate Hass (Persea americana Mill) en estado E3-M (maduro) procedente del Oriente Antioqueño, empleando complejos enzimáticos comerciales. Materiales y métodos. ...

  4. Diagnóstico diferencial das distrofias musculares com referência especial às alterações enzimáticas

    Directory of Open Access Journals (Sweden)

    H. Heick

    1967-06-01

    Full Text Available Depois de apresentar os dados clínicos e genéticos relativos a 221 casos de distrofia muscular progressiva (125 casos do tipo Duchenne, 63 do tipo cintura pélvica e 25 do tipo fácio-escápulo-umeral, os autores analisam os resultados das dosagens sôro-enzimáticas que fizeram; em alguns casos foram pesquisas também as atividades de 25 enzimas em homogeneizados de tecido muscular. Os mesmos exames foram feitos em casos de miopatias de outra etiologia e de distrofias musculares neuropáticas e mielopáticas. Foi verificado que o teor das sôro-enzimas aumenta em todas as formas de distrofia muscular progressiva hereditária. No tipo Duchenne as-atividades enzimáticas são elevadas desde o nascimento das crianças acometidas, precedendo o aparecimento da sintomatologia clínica. Com o progredir da moléstia e com o decorrer dos anos os teores sôro-enzimáticos tendem a diminuir, podendo reduzir-se aos valores normais nas fases finais da moléstia. No tecido muscular não existe diferença significante, no que respeita à composição enzimática, entre os diversos tipos de distrofia muscular progressiva e hereditária. Com exceção de poucas enzimas cuja atividade é maior nos tecidos conjuntivo e adiposo que progressivamente substituem o tecido muscular que entra em atrofia, o teor das mio-enzimas diminui com o progredir da moléstia, como expressão do crescente esgotamento do citoplasma da célula muscular. Nas distrofias musculares neuropáticas e mielopáticas as alterações são idênticas, embora de menor intensidade. Algumas diferenças quantitativas podem ser explicadas pela idade dos pacientes e pela intensidade do processo. A mesma diferença pode ser demonstrada mediante exames histopatológicos: nas formas que acometem crianças (Werdnig-Hoffmann as fibras musculares ainda não denervadas se apresentam com aspecto normal, ao passo que, nas formas do adulto (Kugelberg-Welander, possivelmente pelo desgaste provocado pelos esfor

  5. Enzymatic complex in diets for gold fish fingerlings (Carassius auratusComplexo enzimático na dieta de alevinos de kinguio (Carassius auratus

    Directory of Open Access Journals (Sweden)

    Arcângelo Augusto Signor

    2013-06-01

    Full Text Available Aiming to evaluate the enzymatic complex supplementation in diets for goldfish fingerlings (Carassius auratus, 240 fish weighing initially 1,36 ± 0,02g, randomly distributed in 20 tanks with 150L, in four treatments and five replications, with twelve fish in each experimental unit were used. The fish were fed at 8:00 and 11:00 a.m. and 2:00 and 5:00 p.m. with diets containing different inclusion levels (0; 0,033; 0,066 e 0,099% of enzymatic complex (amilase, protease, celulase, lipase, ?-glucanase and phytase, and formulated with 32,36% of digestible protein and 3.023kcal of digestible energy kg-1. There were no differences observed (P>0,05 in the mean final weight, weight gain, total length, standard length, survival and carcass composition. However, the fish apparent feed conversion was impaired by the supplementation of enzymatic complex with 0,099% in diet. The use of enzymatic complex does not provides benefits in the productive performance for goldfish fingerlings. Objetivando avaliar a suplementação de complexo enzimático em dietas de alevinos de kinguios (Carassius auratus, foram utilizados 240 peixes com peso inicial de 1,36 ± 0,02g, distribuídos aleatoriamente, em 20 tanques de 150L, em quatro tratamentos e cinco repetições, com 12 peixes em cada unidade experimental. Os peixes foram arraçoados as 8h, 11h, 14h e 17h, com dietas contendo diferentes níveis de inclusão (0; 0,033; 0,066 e 0,099% de complexo enzimático (amilase, protease, celulase, lipase, b-glucanase e fitase, e formuladas para conter 32,36% de proteína digestível e 3.023kcal de energia digestível kg-1. Não foram observadas diferenças no peso final, ganho de peso, comprimento total, comprimento padrão, sobrevivência e composição de carcaça. No entanto, a conversão alimentar aparente dos peixes alimentados com rações contendo 0,099% do complexo enzimático foi inferior em relação aos alimentados com as demais dietas. Conclui-se que a utiliza

  6. PENGARUH DEGRADASI ENZIM PROTEOLITIK TERHADAP AKTIVITAS ANGIOTENSIN CONVERTING ENZYME INHIBITOR BEKASAM DENGAN Lactobacillus plantarum B1765 (The Effect of Degradation of Proteolitic Enzyme on Angiotensin Converting Enzyme Inhibitor Activity of Bekasam with Lactobacillus plantarum B1765

    Directory of Open Access Journals (Sweden)

    Prima Retno Wikandari

    2016-10-01

    Full Text Available This research studied the effect of digestive enzyme degradation on the Angiotensin Converting Enzyme Inhibitor (ACEI activity and the stability of bekasam peptide and ACEI activity. Water extract of bekasam was subjected to pepsin and trypsin. The stability of peptide was measured from the changes of peptide concentration before and after treatment by those enzymes. The stability of ACEI activity was measured by hypuric acid liberated from Hip-His-Leu as ACE substrate and determined by spectrophotometer. The results showed that proteolytic enzyme degradation did not affect the concentration of peptide (p>0,05 and the mean concentration 36.72. It was closely related with the ACEI activity that did not change significantly before and after digestion by pepsin and trypsin (p>0,05 and the mean ACEI activity was 70.73. It showed that ACEI activity of bekasam did not change by the degradation of digestive enzyme. Keywords: bekasam, fermented fish, peptides, ACEI activity ABSTRAK Penelitian ini bertujuan untuk mengkaji pengaruh degradasi enzim pencernaan proteolitik terhadap stabilitas peptida dan aktivitas Angiotensin Converting Enzyme Inhibitor (ACEI bekasam yang difermentasi dengan kultur starter Lactobacillus plantarum B1765. Terhadap ekstrak bekasam diberi perlakuan enzim proteolitik pepsin dan tripsin. Pengujian stabilitas peptida diukur dengan ada tidaknya perubahan jumlah peptida setelah perlakuan enzim menggunakan metode formol, sedangkan aktivitas ACEI dilakukan dengan mengetahui jumlah asam hipurat dari substrat Hip-His-Leu yang dibebaskan oleh ACE diukur dengan spektrofotometer. Hasil pengujian menunjukkan perlakuan enzim proteolitik tidak berpengaruh pada konsentrasi peptida dengan p>0,05 dengan nilai rata-rata konsentrasi peptida sebesar 36,72. Hal ini berkorelasi dengan aktivitas ACEI yang juga menunjukkan tidak ada pengaruh antara perlakuan sebelum dan setelah degradasi enzim (p>0,05 dengan rata-rata aktivitas ACEI sebesar 70,73. Hasil

  7. KOEFISIEN KECERNAAN FRAKSI SERAT BUNGKIL KELAPA SAWIT YANG DIHIDROLISIS DENGAN ENZIM ASAL CAIRAN RUMEN DOMBA SEBAGAI PAKAN BENIH IKAN PATIN SIAM (Pangasius hypophthalmus

    Directory of Open Access Journals (Sweden)

    Wahyu Pamungkas

    2012-12-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui nilai kecernaan fraksi serat bungkil kelapa sawit yang dihidrolisis dengan enzim cairan rumen domba. Koefisien kecernaan ditentukan dengan menggunakan indikator Cr2O3 yang ditambahkan dalam pakan uji. Bahan pakan yang digunakan adalah bungkil kelapa sawit (BKS yang dihidrolisis dengan enzim asal cairan rumen domba dengan volume 100 mL/kg BKS dan diinkubasi selama 24 jam (BKSe dan yang tidak dihidrolisis (BKS. Pakan yang digunakan dalam penelitian adalah pakan acuan (pakan komersil, pakan uji A (30% BKSe dan B (30% BKS dengan 3 ulangan. Ikan yang digunakan 10 ekor benih ikan patin siam Pangasius hypophthalmus dengan bobot rata-rata 20 g/ekor yang dipelihara dalam fiber dengan volume air 80 liter. Pemberian pakan secara at satiation dengan frekuensi pemberian 3 kali per hari. Feses dikumpulkan selama 15 hari pemeliharaan untuk dianalisis kandungan nutrisinya. Hasil analisis kecernaan menunjukkan bahwa nilai kecernaan fraksi serat menunjukkan adanya perbedaan yang nyata (P<0,05 antar perlakuan di mana pakan A (30% BKSe mempunyai nilai kecernaan fraksi serat lebih tinggi dibandingkan pakan B (30% BKS.

  8. Indicadores de salud ambiental

    Directory of Open Access Journals (Sweden)

    Manuel Posada de la Paz

    2004-12-01

    Full Text Available Esta ponencia presenta una visión general del proyecto de Indicadores de Salud Ambiental, coordinado por la OMS a nivel internacional y liderado por el Centro de Investigación sobre el Síndrome del Aceite Tóxico y Enfermedades Raras (CISATER en España. En ella se describen los objetivos del proyecto, las gestiones realizadas y los resultados obtenidos durante la fase de viabilidad de este proyecto. El proyecto consiste en el establecimiento de un sistema de información sobre salud ambiental que permita desarrollar una vigilancia de los factores ambientales determinantes de los estados de salud, realizar comparaciones internacionales, elaborar políticas de acción, así como facilitar la comunicación con la ciudadanía. La OMS desarrolló una metodología para el desarrollo de estos indicadores dentro del marco conceptual de información ambiental DPSEEA (Fuerzas impulsoras, Presión, Estado, Exposición, Efecto, Acción y seleccionó un total de 55 indicadores (que incluyen 168 variables sobre 10 áreas de la salud ambiental. Durante la fase de viabilidad se predijo que podrían obtenerse el 89% de los indicadores. Sin embargo la recolección de los datos supuso muchas dificultades debido a la incompatibilidad de algunas variables en los sistemas de información españoles con las variables definidas por la OMS. A nivel de gestión del proyecto, la mayor dificultad radica en la disparidad de responsabilidades en materia de medio ambiente y salud entre las instituciones españolas. Además de la aportación técnica a la salud ambiental en España, un valor añadido de este proyecto ha sido el establecimiento de líneas de colaboración estrechas con los responsables de los diferentes Ministerios implicados.

  9. Ambient Air Quality Data Inventory

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Office of Air and Radiation's (OAR) Ambient Air Quality Data (Current) contains ambient air pollution data collected by EPA, other federal agencies, as well as...

  10. Caracterização de acessos de pimenta-do-reino com base em sistemas enzimáticos Characterization of black pepper accessions using isozymes

    Directory of Open Access Journals (Sweden)

    José MD Gaia

    2007-09-01

    Full Text Available Setenta e oito acessos de pimenta-do-reino, incluindo algumas espécies silvestres foram submetidos à análise eletroforética de isoenzimas em gel de poliacrilamida, visando distinguir diferenças fenotípicas que auxiliem na discriminação e seleção dos acessos. Foram utilizados os sistemas enzimáticos SKDH, GOT, ACP, ACO, PGI, FUM, 6PGDH e G6PDH. O polimorfismo de isoenzimas foi avaliado pelo número de alozimas com diferentes mobilidades por sistema enzimático, pelas freqüências de alozimas dentro de cada sistema enzimático em relação ao total de bandas do sistema e pela análise da similaridade genética, com base na ausência e presença de bandas. Todos os sistemas enzimáticos utilizados tiveram boa resolução e definição de bandas, com ênfase para SKDH, 6PGDH, PGI e ACP. Em sua totalidade, os sistemas apresentaram polimorfismo capaz de caracterizar e identificar acessos ou grupos de pequeno número de acessos, sendo que o sistema GOT foi o que apresentou maior variabilidade de alozimas e de perfis; e o que apresentou menor variabilidade foi o sistema FUM, com três alozimas e quatro perfis. Cinqüenta e sete por cento das alozimas podem ser usadas para caracterizar e identificar clones ou grupos de clones. Cerca de 64% dos acessos analisados podem ser identificados por um a seis fenótipos individuais de sistemas enzimáticos. A análise da similaridade indicou os grupos G1, G2 e G3 como os mais divergentes da coleção, os quais são indicados para cruzamentos intraespecíficos e interespecíficos visando a obtenção de clones superiores.Seventy and eight accessions of black pepper, including some wild species, were analyzed through isozyme electrophoresis in polyacrylamide gel, aiming to distinguish phenotypic differences to discriminate and select accessions. The enzymatic systems SKDH, GOT, ACO, ACP, PGI, FUM, 6PGDH and G6PDH were studied. The polymorphism of isozymes was evaluated based on number of alozymes with

  11. Alterações enzimáticas decorrentes de isquemia muscular esquelética em ratos

    Directory of Open Access Journals (Sweden)

    Ely Jorge Fonseca

    2000-01-01

    Full Text Available Os autores estudaram as alterações enzimáticas [transaminase oxalo-acética (TGO, creatinofosfoquinase (CPK e desidrogenase lática (LDH] decorrentes de isquemia muscular esquelética em ratos submetidos à isquemia de membro inferior por 0, 2, 4 e 6 horas. Vinte e oito ratos Wistar foram divididos em 4 grupos: Grupo I (controle; e Grupos II, III, e IV (isquemia por 2, 4 e 6 horas, respectivamente. Após o período de isquemia, foi coletado sangue do plexo retrocular para análise laboratorial. Os valores obtidos (média±desvio padrão para TGO nos Grupos I, II, III e IV foram, respectivamente: 20,14±6,76; 59,71±28,91; 88,28±11,17 e 123±52,65 U/l. Para CPK, os valores foram: 67,85±62,76; 203±108,71; 237,71±95,06 e 291,71±173,19 U/l. Para LDH, obteve-se: 334,14±117,13; 414,42±222,47; 526±234,75 e 427,57±273,58 U/l. Para análise estatística, utilizou-se o método de ANOVA, seguido do teste t de Bonferroni (p<0,05. Os níveis de TGO foram significativamente superiores nos grupos III e IV quando comparados ao controle. Para CPK, houve distinção significativa entre os grupos IV e I. Os níveis de LDH não se demonstraram estatisticamente diferentes entre os grupos. Os autores concluem que em períodos de isquemia por 2, 4 e 6 horas, a dosagem de LDH não se mostra um parâmetro útil na avaliação de dano muscular esquético. A TGO e CPK tiveram alterações significativas a partir de 4 e 6 horas, respectivamente, mostrando-se úteis na avaliação de isquemia muscular em ratos.

  12. Estudo da atividade inibitória enzimática da quercetina sobre a resposta vasodepressora induzida pela bradicinina

    Directory of Open Access Journals (Sweden)

    Luciane Pereira Nascimento Häckl

    Full Text Available Em estudos recentes tem sido sugerido que os flavonóides, compostos de origem vegetal sendo encontrados em vários produtos alimentares, possuem uma ação inibitória de vários sistemas enzimáticos. A inibição da enzima conversora da angiotensina (ECA tem sido utilizada no tratamento da hipertensão. O mecanismo de ação dos inibidores da ECA implica na formação da Angiotensina II e na degradação da bradicinina. O propósito do presente estudo foi de analisar a ação da quercetina na atividade da ECA através da avaliação do efeito da angiotensina I (AI e da bradicinina (BK na pressão arterial de ratos anestesiados. Foram utilizados neste estudo ratos Wistar machos adultos. Estes animais foram anestesiados com uretana e foi introduzida na traquéia uma cânula de polietileno (PE 240 para facilitar a respiração. Também foi isolada e canulada (PE 50 a veia jugular para administração das substâncias em estudo. Para registro da pressão arterial foi introduzida na artéria carótida comum esquerda uma cânula de polietileno (PE 50 acoplada a um transdutor de pressão. A administração de BK (10 nmol/kg, i.v. induziu um efeito hipotensor, enquanto que a administração de AI (0,1 nmol/kg induziu uma resposta hipertensora. Pré-tratamento com captopril v.o. (100 nmol/kg 45 min antes e i.v. (10 nmol/kg 5 min antes aumentou significativamente a resposta à BK; embora captopril reduziu, também significativamente, o efeito da AI. O tratamento com quercetina via oral (30 mg/kg 45 min antes e via intravenosa (5 mg/kg 5 min antes aumentou significativamente o efeito da BK. O efeito da AI foi significativamente reduzido por ambos os tratamentos. Estes resultados sugerem que a quercetina potenciou o efeito hipotensor da bradicinina e reduziu o efeito hipertensor da angiotensina I através de ação inibitória sobre a enzima conversora da angiotensina, portanto apresentando um mecanismo de ação semelhante ao captopril.

  13. Medio ambiente urbano

    OpenAIRE

    Rodríguez-Chaves Mimbrero, Blanca

    2007-01-01

    El estudio  y análisis  de las interacciones  entre  ambiente  y desarrollo y  su inserción  en los procesos  de  planificación del crecimiento  social y económico  de  los  países  de América Latina, reviste especial interés para proponer alternativas de acción que  conduzcan  al  logro  de  una mejor  calidad de  vida.  El impacto  que las conferencias sobre  el  Medio Ambiente Humano Estocolmo (1972),  Cocoyoc  (1974) o de documentos como "Nuestro Futuro Común" o "Nuestra Propia Agenda" ha...

  14. NIF Ambient Vibration Measurements

    International Nuclear Information System (INIS)

    Noble, C.R.; Hoehler, M.S.; S.C. Sommer

    1999-01-01

    LLNL has an ongoing research and development project that includes developing data acquisition systems with remote wireless communication for monitoring the vibrations of large civil engineering structures. In order to establish the capability of performing remote sensing over an extended period of time, the researchers needed to apply this technology to a real structure. The construction of the National Ignition Facility provided an opportunity to test the data acquisition system on a large structure to monitor whether the facility is remaining within the strict ambient vibration guidelines. This document will briefly discuss the NIF ambient vibration requirements and summarize the vibration measurements performed during the Spring and Summer of 1999. In addition, a brief description of the sensors and the data acquisition systems will be provided in Appendix B

  15. MEIO AMBIENTE E DESENVOLVIMENTO

    Directory of Open Access Journals (Sweden)

    Suely Salgueiro Chacon

    2009-12-01

    Full Text Available O objetivo deste artigo é resgatar elementos para subsidiar uma reflexão crítica sobre o modelo de desenvolvimento econômico prevalente na sociedade e as relações com o meio ambiente, sob a ameaça que ronda o destino da espécie humana, conforme afirmação de Lovelock (2006, p. 20 sobre o conceito de desenvolvimento sustentável: “uma ideia adorável se a tivéssemos aplicado 200 anos atrás, quando havia um bilhão de pessoas no mundo. Agora é tarde demais. Não há mais espaço para nenhum tipo de desenvolvimento. A humanidade tem que regredir”. Este artigo apresenta a evolução do conceito de desenvolvimento econômico sob a ótica da sustentabilidade, e interliga temas como: o ambientalismo, aglutinador de distintos pensamentos sobre as relações entre a sociedade e a natureza; o movimento ambiental, a fundamentar a disseminação do conceito de desenvolvimento sustentável, e a gestão ambiental, abordada como prática orientada pelo conceito de desenvolvimento sustentável.

  16. Application of pH regulator 65 to wet processing of textiles%pH值控制剂65在印染加工中的应用

    Institute of Scientific and Technical Information of China (English)

    王力民; 李春光; 苏长智; 张万伟; 秦新波

    2012-01-01

    pH值控制剂65系有机酯和有机羧酸盐复配而成,它能够完全中和纤维内部的碱剂而不损伤织物强力,将布面的pH值控制在7.0~7.5.pH值控制剂65可解决敏感色织物、增白织物的pH值控制以及织物泛黄等问题,且与各种功能性整理剂相容性好.%pH regulator 65 is a complex of organic ester and organic carboxylate. The pH regulator 65 can completely neutralize the base in the internal fiber and stablize the pH value of fabric surface at 7~7.5 without strength loss. It can solve the problems of pH controlling and fabric yellowing in processing of sensitive and whitening fabric, and has good compatibility with all kinds of functional finishing agents.

  17. Tratamiento enzimático en la extracción de aceite de pipa de uva, Vitis vinifera, por prensado en frío

    Directory of Open Access Journals (Sweden)

    Zúñiga, M. E.

    2003-03-01

    Full Text Available The effect of enzyme incorporation is analyzed over the yield of grapeseed oil extraction by cold pressing. By this type of process the objective is to preserve grapeseed active compounds and to diminish the environmental impact in comparison to oil extraction conventional methods which use higher temperatures. The enzymatic hydrolysis takes place with a commercial enzyme previous to pressing stage. Its effectiveness is compared to a control without the biocatalyst. An oil extraction yield of 72 % d.b. is obtained with an enzymatic pre-treatment for 4 hours at 50 ºC, and 60 % of moisture, increasing in 26 % the control result. It is concluded that the incorporation of an enzymatic processing would be a feasible alternative to extract grapeseed oil under softener process conditions improving the yield of extraction in comparison to the same type of methodology without an enzyme.Se analiza el efecto de la incorporación de enzimas sobre el rendimiento de extracción de aceite por prensado en frío. Con el prensado en frío se busca preservar los compuestos activos de la pipa de uva y minimizar el impacto medioambiental en comparación con los métodos convencionales de extracción de aceite que utilizan altas temperaturas. El tratamiento enzimático se efectúa previo al prensado con una enzima comercial y su efectividad se compara con un control sin catalizador. Un rendimiento de extracción de aceite del 72 % b.s. se logra con un pre-tratamiento enzimático durante 4 horas a 50 ºC, y 60 % de humedad, superando en un 26 % al control. Se concluye que la incorporación de un tratamiento enzimático sería una alternativa factible para extraer el aceite de pipa de uva bajo condiciones suaves de proceso mejorando el rendimiento de extracción en comparación con el mismo tipo de proceso sin enzima.

  18. Dioxinas y medio ambiente

    Directory of Open Access Journals (Sweden)

    María Teresa Frejo Moya

    2011-12-01

    Full Text Available Con el término genérico dioxinas se designa al grupo de las dibenzo-p-dioxinas policloradas (PCDD y de los dibenzofuranos policlorados (PCDF, representantes típicos de los compuestos orgánicos persistentes (COPs. Se obtienen como productos secundarios no deseados de diversos procesos industriales en los que se emplea cloro en alguna de sus etapas. Las dioxinas han centrado en la última década una parte importante de la investigación médica en salud ambiental debido a su notable toxicidad, ya que son las sustancias químicas peligrosas más potentes creadas por el hombre, afectando al sistema nervioso e inmunitario, estando implicadas en la aparición de distintos tipos de cáncer y provocando la aparición de alteraciones hormonales, clasificándose actualmente como disruptores endocrinos. Por otra parte, su persistencia en el medio ambiente, resistencia a la degradación, bioacumulación y capacidad de transporte atmosférico entre las diversas fases medioambientales hace que sean considerados actualmente como compuestos peligrosos para el ser humano.

  19. Preparação regiosseletiva de derivados acilados da D-ribono-1,4-lactona empregando catálise enzimática

    OpenAIRE

    Sebrão, Damianni

    2011-01-01

    Tese (doutorado) - Universidade Federal de Santa Catarina, Centro de Ciências Físicas e Matemáticas, Programa de Pós-Graduação em Química, Florianópolis, 2011 Nesta tese foram desenvolvidas metodologias enzimáticas para a preparação regiosseletiva de derivados acilados da D-ribono-1,4-lactona. Os produtos foram identificados e quantificados por RMN-1H, UV-Vis ou titulação volumétrica com solução aquosa de KOH. Várias lipases, uma protease e dois micélios isolados de fungos da região amazôn...

  20. Determinación de Glifosato mediante inmunoensayo enzimático (ELISA en el Paisaje Protegido Laguna de Rocha y su entorno, Uruguay.

    Directory of Open Access Journals (Sweden)

    Daniela Nardo

    2015-12-01

    Full Text Available En el entorno de la Laguna de Rocha se ha visto incrementada la superficie dedicada a las actividades agrícolas con un mayor uso de plaguicidas, entre ellos el herbicida glifosato, usado en cultivos de soja principalmente. Mediante la utilización de técnicas de inmunoensayo enzimático (ELISA, se investigó la presencia de glifosato en la Laguna y algunos de sus afluentes en dos momentos específicos de tiempo. Se detectó glifosato en 27 de las 28 muestras estudiadas. Muestras positivas por ELISA fueron confirmadas por cromatografía iónica. El método ELISA demostró ser una herramienta de screening adecuada para determinar la presencia de glifosato en agua.

  1. Types for BioAmbients

    Directory of Open Access Journals (Sweden)

    Sara Capecchi

    2010-02-01

    Full Text Available The BioAmbients calculus is a process algebra suitable for representing compartmentalization, molecular localization and movements between compartments. In this paper we enrich this calculus with a static type system classifying each ambient with group types specifying the kind of compartments in which the ambient can stay. The type system ensures that, in a well-typed process, ambients cannot be nested in a way that violates the type hierarchy. Exploiting the information given by the group types, we also extend the operational semantics of BioAmbients with rules signalling errors that may derive from undesired ambients' moves (i.e. merging incompatible tissues. Thus, the signal of errors can help the modeller to detect and locate unwanted situations that may arise in a biological system, and give practical hints on how to avoid the undesired behaviour.

  2. Evaluación de tiempos quirúrgicos y transfusiones en pacientes quemados tratados con desbridamiento enzimático

    Directory of Open Access Journals (Sweden)

    Ángela Ojeda-Regidor

    Full Text Available Resumen Introducción y Objetivo En los últimos años se han publicado resultados que demuestran un desbridamiento enzimático rápido, eficaz y específico de tejidos quemados con el uso de Nexobrid® (Mediwound, Alemania, producto compuesto por un concentrado de enzimas proteolíticas enriquecidas con bromelaína. En este estudio comparamos el uso de Nexobrid® como desbridante enzimático frente a un Grupo Control con tratamiento estándar, con el objetivo de evaluar; en función de la superficie corporal quemada (SCQ, la cantidad de intervenciones quirúrgicas realizadas, la colonización del lecho quirúrgico, la necesidad de escarotomías, así como los requerimientos transfusionales de los pacientes tratados. Material y Método Estudio retrospectivo con 65 pacientes, edad media de 46.87 años, tratados con Nexobrid® entre septiembre de 2015 y diciembre de 2016. Comparamos con un grupo control de 177 pacientes, edad media de 48.24 años, intervenidos mediante desbridamiento tangencial desde enero a diciembre de 2014. El Grupo Control presenta unas características homogéneas a las del Grupo Nexobrid®. Ambos grupos fueron estratificados en función de su SCQ mayor o menor del 15%. Resultados Encontramos diferencias estadísticamente significativas (p 15%. Los requerimientos transfusionales fueron menores en el Grupo Nexobrid® frente al Grupo Control en los pacientes con SCQ > 15% (p 15%. Finalmente, reduce la necesidad de transfusión sanguínea en pacientes con SCQ mayor del 15%.

  3. Volátiles generados por hidrólisis enzimática de glicósidos de hojas de lulo (Solanum quitoense L.

    Directory of Open Access Journals (Sweden)

    Carolina Osorio R.

    2010-07-01

    Full Text Available La fracción glicosídica de hojas de lulo (Solanum quitoense L. fue obtenida por adsorción sobre Amberlita XAD-2 y posterior elución con metanol. Los glicósidos así extraídos se hidrolizaron enzimáticamente con una glicosidasa no selectiva (Rohapect D5-L y las agliconas (volátiles enlazados glicosídicamente producidas fueron caracterizadas químicamente por CGAR (cromatografía de gases de alta resolución, CGAR-EM (cromatografía de gases de alta resolución acoplada a espectrometría de masas y analizadas sensorialmente (nota olfativa por CGAR-O (cromatografía de gases de alta resolución acoplada a olfatometría. Se detectaron en total 25 compuestos volátiles y de ellos, se identificaron 21, los cuales representan el 90 % de todas las agliconas generadas. Los volátiles identificados presentaron diversas estructuras químicas que pueden agruparse en Ci3-norisoprenoides (38.1 %, 15 mg/kg hojas, compuestos aromáticos (30.5 %, alcoholes monoterpénicos (12.3 % y compuestos alifáticos Cg (9.3 %. El extracto de volátiles producido por hidrólisis enzimática presentó un olor a verde amaderado agradable, con fuertes notas dulces parecidas a la miel.

  4. Reforma constitucional y ambiente

    Directory of Open Access Journals (Sweden)

    Teodoro Bustamante

    2013-09-01

    Full Text Available América Latina está atravesada por una ola de reformas constitucionales. Sus causas, las expectativas que ellas despiertan, los riesgos que se han asociado al proceso de lucha política en su entorno, son temas de un análisis fundamentalmente político; pero hay algunos aspectos en los cuales ese debate tiene una directa repercusión sobre el tema ambiental. En el caso del Ecuador, esto se refleja en el hecho de que una de las innovaciones que se proponen, se refieren a una nueva forma de abordar los temas ambientales, básicamente se establecen Derechos de la Naturaleza.

  5. Descripción de un inmunoensayo enzimático que reconoce anticuerpos anti-factor VIII/von Willebrand obtenidos a partir de hibridomas múridos Description of an enzime immunoassay that recognizes anti-factor VIII/von Willebrand antibodies obtained from murine hybridomas

    Directory of Open Access Journals (Sweden)

    Renée González Sampedro

    1999-04-01

    Full Text Available Se describe un inmunoensayo enzimático (ELISA que detecta anticuerpos anti-Factor-VIII/von Willebrand (FVIII/vW, con una relación de enlace > 5, que permite reconocer dichos anticuerpos tempranamente en los cultivos de hibridomas múridos. Para la adsorción de las placas del inmunoensayo se utilizaron preparados semipurificados de FVIII/vW obtenidos en Cuba. Se inmunizaron ratones Balb/c con la finalidad de generar los anticuerpos monoclonales detectados por este ELISA. Se obtuvo un clon con crecimiento estable y varios subclones positivos por el ELISA con actividad biológica específica de anticuerpos inhibidores de FVIIIAn enzime immunoassay (ELISA that detects anti-Factor VIII/von Willebrand (FVIII/vw antibodies with a binding relationship > 5 that allows the early recognition of such antibodies in the cultures of murine hybridomas is described. Semipurified preparations of FVIII/vw obtained in Cuba were used for the adsorption of the immunoassay plates. Balb/c mice were immunized in order to generate the monoclonal antibodies detected by this ELISA. A clone with stable growth and several positive subclones were obtained by ELISA with specific biological activity of FVIII-inhibiting antibodies

  6. Validating Firewalls in Mobile Ambients

    DEFF Research Database (Denmark)

    Nielson, Flemming; Nielson, Hanne Riis; Hansen, René Rydhof

    1999-01-01

    The ambient calculus is a calculus of computation that allows active processes (mobile ambients) to move between sites. A firewall is said to be protective whenever it denies entry to attackers not possessing the required passwords. We devise a polynomial time algorithm for rejecting proposed...

  7. IMPACTO DEL ARSÉNICO EN EL AMBIENTE Y SU TRANSFORMACIÓN POR MICROORGANISMOS

    Directory of Open Access Journals (Sweden)

    Edelweiss Airam Rangel Montoya

    2015-04-01

    Full Text Available El arsénico (As es uno de los metaloides más tóxicos presentes en el medio ambiente y la especiación de éste depende de diversos factores químicos, físicos y biológicos. La distribución y contaminación del arsénico se debe a procesos naturales y antropogénicos, y su problemática se debe a su fácil movilización en el ambiente. Las altas concentraciones de arsénico en agua y suelo se han convertido en un problema global, ya que las exposiciones prolongadas a este metaloide pueden causar daños crónicos a la salud. Dicha situación es particularmente importante en la Comarca Lagunera en México. Por lo tanto, se ha vuelto necesaria la búsqueda de nuevas estrategias para la remoción de este metaloide, entre las cuales, la biorremediación microbiana ha cobrado importancia como una alternativa amigable con el ambiente en la solución de este problema. Los microorganismos juegan un papel fundamental en la especiación del arsénico, ya que un gran número de estos tiene la capacidad de transformar el arsénico a pesar de su toxicidad puesto que han desarrollado diferentes mecanismos que les permiten utilizar el arsénico en su metabolismo, ya sea en su forma reducida de arsenito, o en su forma oxidada de arseniato mediante reacciones de óxido-reducción transformación enzimática, metilación, quelación, exclusión e inmovilización. Este trabajo, trata sobre el impacto que tiene la especiación de arsénico, especialmente del arsenito (As III, en el medio ambiente, el riesgo que representa la contaminación para la salud, así como los mecanismos bioquímicos microbianos para la oxidación del arsenito, disminuir su toxicidad y el desarrollo de estrategias limpias y amigables con el medio ambiente para la remoción de arsénico de aguas y suelos contaminados.

  8. Salud ambiental: conceptos y actividades

    Directory of Open Access Journals (Sweden)

    Gonzalo A. Ordóñez

    2000-03-01

    Full Text Available La finalidad del trabajo es aportar información y propuestas conceptuales que faciliten la tarea de quienes tienen a su cargo la sistematización institucional de la salud ambiental. Se hace un análisis de la noción de "ambiente" para la cual se sugiere una definición, y se examina el lugar de la salud ambiental en el contexto de los problemas ambientales y sus vertientes "verde" y "azul". Se examinan denominaciones equivalentes de salud ambiental y se introducen los servicios de salud ambiental. Se proporcionan varias definiciones y se da la oficial de salud ambiental adoptada por la OMS en Sofía, Bulgaria (1993. A continuación se transcriben las áreas básicas que a la salud ambiental le han asignado diversas organizaciones o reuniones, como la OPS, la OMS, el Programa 21 y otros. A partir de aquí se construye un repertorio bastante completo de áreas y subáreas y se encuentra que todos los listados son, en realidad, una reunión asistemática de tres tipos de constituyentes: determinantes (factores o hechos de la realidad física, procesos (conjuntos de intervenciones y funciones (conjuntos de acciones de gestión, los cuales pueden enfocarse matricialmente y llevan a individualizar actividades de los servicios de salud ambiental. Se proponen unas reglas de operación que permiten, en una especie de álgebra, construir expresiones para especificar con precisión las actividades y sus agregados. De este modo se logra disponer de un lenguaje simbólico común que puede ayudar a la intercomunicación, enseñanza e investigación en el ámbito de la salud ambiental.

  9. Aspectos imunológicos do sistema enzimático fenoloxidase de Schistosoma mansoni Immunological aspects of the phenol oxidase enzymatic system of Schistosoma mansoni

    Directory of Open Access Journals (Sweden)

    João Tadeu Ribeiro-Paes

    1994-10-01

    Full Text Available O sistema enzimático fenoloxidase (EC 1.10.3.1, EC 1.10.3.2 está amplamente distribuido entre os seres vivos, tendo sido descrito em diferentes espécies do reino animal e vegetal. Apesar de desempenhar um papel fundamental na formação da cápsula ou parede dos ovos de trematódeos, o sistema enzimático fenoloxidase (PO tem sido pouco estudado nesses organismos. No presente trabalho são apresentados os resultados iniciais de imunizações de coelhos contra PO de fêmeas adultas de S. mansoni e tirosinase de cogumelo (Sigma. As análises imunológicas, realizadas através de imunodifusão dupla (teste de Ouchterlony e imunoeletroforese, revelaram identidade imunitária parcial entre a PO de machos e fêmeas. Não se observou reação cruzada entre os antissoros de coelhos imunizados contra PO e aqueles com tirosinase, indicando que, embora os sítios catalíticos de ambas as enzimas devam ser semelhantes, já que atuam sobre os mesmos substratos, os determinantes antigênicos devem ser diferentes. Os resultados descritos no presente trabalho representam um primeiro passo no sentido da purificação das isoenzimas da fenoloxidase e sua posterior utilização ao estudo dos mecanismos moleculares envolvidos na esclerotização da parede dos ovos de S. mansoni.The phenol oxidase enzymatic system (EC 1.10.3.1, EC 1.10.3.2 is widespread in different species of the animal and vegetal kingdom. Despite its importance in the eggshell formation of the trematodes phenol oxidase (PO has been little studied in these organisms, mainly in S. mansoni. This report presents the initial results concerning the immunization of rabbits with PO of S. mansoni and mushroom tyrosinase. The immunological analysis done by means of double immu-nodifusion (Ouchterlony and immunoelectrophoresis techniques revealed some immunological identity between the PO of males and females. It was not seen cross reaction between the antisera against PO and tyrosinase, what suggests

  10. Guía clínica de consenso en el uso de desbridamiento enzimático en quemaduras con NexoBrid®

    Directory of Open Access Journals (Sweden)

    José-Ramón Martínez-Méndez

    Full Text Available Introducción y Objetivo: El desbridamiento precoz es la base del tratamiento de las quemaduras. La retirada de la escara durante las primeras 72 horas es la mejor opción para reducir la estancia hospitalaria y los eventos infecciosos. Sin embargo, en muchas ocasiones se compromete la dermis viable necesaria para obtener los mejores resultados estéticos y funcionales, obligando a injertar el lecho. Hay numerosa evidencia acerca de la reducción de las tasas de injerto, la pérdida hemática y el número de intervenciones cuando se utiliza un desbridante enzimático, NexoBrid®. El objetivo de esta publicación es establecer una guía clínica basada en la opinión de los expertos españoles. Material y Método: Se diseñó un panel de 7 expertos de las principales Unidades de Quemados españolas, con más de 350 pacientes tratados, que discutió las diferentes fases del tratamiento con Nexo-Brid® para obtener una guía clínica de consenso acerca de la indicación, uso y manejo del desbridamiento enzimático. Resultados: Se alcanzó un alto nivel de consenso, con más del 70% de acuerdo en cada una de las fases de tratamiento. Todos los aspectos del tratamiento con NexoBrid® fueron discutidos durante la reunión, así como las indicaciones y limitaciones de su uso, incluyendo todas las nuevas evidencias publicadas hasta el momento. También las diversas opciones utilizadas por los diferentes centros de quemados españoles, alcanzando una recomendación global sobre su uso. Conclusiones: Se redactó un documento como guía clínica preliminar sobre el uso de NexoBrid® hasta que se creen nuevas guías basadas en evidencia. No existe ningún otro consenso similar publicado hasta el momento.

  11. Experiencia con Nexobrid® para el desbridamiento enzimático de quemaduras faciales seguido de tratamiento conservador con Medihoney®

    Directory of Open Access Journals (Sweden)

    José-Manuel Sampietro-De-Luis

    Full Text Available Introducción y Objetivos. El desbridamiento enzimático de las quemaduras ha sido objeto de estudio durante décadas; en este terreno, NexoBrid® (MediWound Ltd., Israel se postula como una prometedora alternativa al desbridamiento quirúrgico clásico. Por otra parte, las propiedades antibacterianas y promotoras de la cicatrización de la miel también se han evaluado recientemente con buenos resultados clínicos. Describimos nuestra experiencia preliminar con el empleo de NexoBrid® para el tratamiento de quemaduras faciales, seguido de curas tópicas con Medihoney® Wound Gel (Derma Sciences Ltd., EE.UU.. Material y Método. Incluimos en el estudio todos los pacientes atendidos en nuestra Unidad de Quemados con quemaduras faciales dérmicas o subdérmicas que afectaban a 2 o más subunidades estéticas. Realizamos desbridamiento enzimático con NexoBrid® en las primeras 24 horas. Posteriormente, llevamos a cabo tratamiento conservador con curas tópicas con Medihoney® Wound Gel. Recogimos todos los datos de calidad del desbridamiento, necesidad de desbridamiento quirúrgico, tiempo hasta epitelización completa, presencia de infección y necesidad de cirugía correctora de secuelas. Resultados. Tratamos 10 pacientes con quemaduras faciales de etiología diversa (llama, flash eléctrico, deflagración, escaldadura y química. El desbridamiento inicial fue completo en todos los pacientes. Se alcanzó la epitelización completa en una media de 13.88 días (10-20 días. Ningún paciente presentó infección clínicamente manifiesta ni precisó desbridamiento quirúrgico, cobertura mediante autoinjertos o cirugía de secuelas. Conclusiones. Nuestra experiencia preliminar indica que parece factible la aplicación de NexoBrid® y su combinación con Medihoney® para el tratamiento conservador de las quemaduras faciales.

  12. Purificación y determinación de actividad enzimática de la catalasa en Staphylococcus aureus

    Directory of Open Access Journals (Sweden)

    Iván Marin Arguello

    2000-02-01

    Full Text Available Ampliamente distribuida en células animales, vegetales y micro- organismos, la catalasa es una enzima crucial para la vida. Su función catalítica es la descomposición del peróxido de hidrógeno (HOY, compuesto altamente tóxico y generado en los procesos metabólicos de la célula. Esta enzima en Staphylococcus aureus es una proteína tetramérica presentando grupos hemos y moléculas de NADPH en su estructura. La determinación espectrofotométrica de los parámetros cinéticos (Km, Vmax. y otros en dependencia del pH y temperatura nos podría permitir regular su actividad enzimática. La enzima manifiesta un Km de 50mM y la presencia de dos aminoácidos (Histidina y Cisteína en el sitio activo aparentemente son fundamentales en el proceso de catálisis.

  13. Conhecimento, interdisciplinaridade e Psicologia Ambiental

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    Ombretta Romice

    2005-01-01

    Full Text Available Responde às questões - como os métodos da Psicologia Ambiental devem ser discutidos em um enquadramento interdisciplinar; a Psicologia Ambiental pede alguma abordagem metodológica especial; como a intervenção ambiental é determinada pela interdisciplinaridade; quais são estas disciplinas e como elas se relacionam entre si - baseando-se em experiências profissionais como orientador em um projeto com comunidade, com habitação popular e exclusão social em vários países da Europa, e como consultora. Conclui que as abordagens usadas pelas diferentes profissões são muito separadas, e que apenas metas comuns não são suficientes, sendo também necessários um treino conjunto e identidade de valores.

  14. Ambient cosmology and spacetime singularities

    International Nuclear Information System (INIS)

    Antoniadis, Ignatios; Cotsakis, Spiros

    2015-01-01

    We present a new approach to the issues of spacetime singularities and cosmic censorship in general relativity. This is based on the idea that standard 4-dimensional spacetime is the conformal infinity of an ambient metric for the 5-dimensional Einstein equations with fluid sources. We then find that the existence of spacetime singularities in four dimensions is constrained by asymptotic properties of the ambient 5-metric, while the non-degeneracy of the latter crucially depends on cosmic censorship holding on the boundary. (orig.)

  15. Ambient cosmology and spacetime singularities

    CERN Document Server

    Antoniadis, Ignatios

    2015-01-01

    We present a new approach to the issues of spacetime singularities and cosmic censorship in general relativity. This is based on the idea that standard 4-dimensional spacetime is the conformal infinity of an ambient metric for the 5-dimensional Einstein equations with fluid sources. We then find that the existence of spacetime singularities in four dimensions is constrained by asymptotic properties of the ambient 5-metric, while the non-degeneracy of the latter crucially depends on cosmic censorship holding on the boundary.

  16. TERCEIRO SETOR E MEIO AMBIENTE

    OpenAIRE

    MELO, MARINA FÉLIX DE

    2012-01-01

    Objetivamos discutir, brevemente, como têm se dado as discussões sobre o Terceiro Setor brasileiro e, particularmente, sobre as ONGs que atuam em defesa do meio ambiente, levantando questionamentos acerca das limitações enfrentadas pelo Terceiro Setor neste contexto

  17. La crisis del medio ambiente

    Directory of Open Access Journals (Sweden)

    Juan Carlos Quintero Vélez

    2013-07-01

    Full Text Available Este artículo, introducción al tema del medio ambiente, pretende proporcionar conceptos básicos para analizar y dimensionar el impacto que genera el hombre sobre los sistemas que soportan la vida. Para entender estos problemas, es indispensable partir de un análisis básico de la relación entre el hombre actual, su medio ambiente, sus necesidades y sus actividades. El autor revisa los antecedentes, las causas y las consecuencias de la crisis ambiental internacional, e intenta dar explicación a la problemática nacionalen este campo, y establecer los puntos más críticos en Colombia. Finalmente, con base en los parámetros establecidos por el gobierno, se presenta el concepto de“desarrollo sostenible" como modelo que interrelaciona los procesos económicos, sociales y tecnológicos con el medio ambiente.

  18. The persuasiveness of ambient intelligence

    NARCIS (Netherlands)

    Aarts, E.H.L.; Markopoulos, P.; Ruyter, de B.E.R.; Petkovic, M.; Jonker, W.

    2007-01-01

    Ambient intelligence (AmI) is a novel concept for embedded computing that builds on the large-scale integration of electronic devices into peoples’ surroundings and the ubiquitous availability of digital information to the users of such environments. The concept however is not only concerned with

  19. Ambient intelligence : visualising the future

    NARCIS (Netherlands)

    Aarts, E.H.L.

    2005-01-01

    Ambient Intelligence systems are aimed at making user-system interaction and content consumption a truly positive experience. The endless search for nifty information visualisation mechanism to squeeze yet one more piece of information onto a visual display is surpassed by the challenge to embed

  20. Manufactured Porous Ambient Surface Simulants

    Science.gov (United States)

    Carey, Elizabeth M.; Peters, Gregory H.; Chu, Lauren; Zhou, Yu Meng; Cohen, Brooklin; Panossian, Lara; Green, Jacklyn R.; Moreland, Scott; Backes, Paul

    2016-01-01

    The planetary science decadal survey for 2013-2022 (Vision and Voyages, NRC 2011) has promoted mission concepts for sample acquisition from small solar system bodies. Numerous comet-sampling tools are in development to meet this standard. Manufactured Porous Ambient Surface Simulants (MPASS) materials provide an opportunity to simulate variable features at ambient temperatures and pressures to appropriately test potential sample acquisition systems for comets, asteroids, and planetary surfaces. The original "flavor" of MPASS materials is known as Manufactured Porous Ambient Comet Simulants (MPACS), which was developed in parallel with the development of the Biblade Comet Sampling System (Backes et al., in review). The current suite of MPACS materials was developed through research of the physical and mechanical properties of comets from past comet missions results and modeling efforts, coordination with the science community at the Jet Propulsion Laboratory and testing of a wide range of materials and formulations. These simulants were required to represent the physical and mechanical properties of cometary nuclei, based on the current understanding of the science community. Working with cryogenic simulants can be tedious and costly; thus MPACS is a suite of ambient simulants that yields a brittle failure mode similar to that of cryogenic icy materials. Here we describe our suite of comet simulants known as MPACS that will be used to test and validate the Biblade Comet Sampling System (Backes et al., in review).

  1. Nanomaterials vs Ambient Ultrafine Particles

    DEFF Research Database (Denmark)

    Stone, Vicki; Miller, Mark R.; Clift, Martin J. D.

    2017-01-01

    BACKGROUND: A rich body of literature exists that has demonstrated adverse human health effects following exposure to ambient air particulate matter (PM), and there is strong support for an important role of ultrafine (nanosized) particles. At present, relatively few human health or epidemiology ...

  2. Abstract Interpretation of Mobile Ambients

    DEFF Research Database (Denmark)

    Hansen, René Rydhof; Jensen, J. G.; Nielson, Flemming

    1999-01-01

    We demonstrate that abstract interpretation is useful for analysing calculi of computation such as the ambient calculus (which is based on the p-calculus); more importantly, we show that the entire development can be expressed in a constraint-based formalism that is becoming exceedingly popular...

  3. Shape analysis for Mobile Ambients

    DEFF Research Database (Denmark)

    Nielson, Hanne Riis; Nielson, Flemming

    2000-01-01

    The ambient calculus is a calculus of computation that allows active processes to move between sites. We present an analysis inspired by state-of-the-art pointer analyses that safety and accurately predicts which processes may turn up at what sites during the execution of a composite system. The ...... are flexible and scale up to general tree structures admitting powerful restructuring operations....

  4. Ambient intelligence, ethics and privacy

    NARCIS (Netherlands)

    Hoof, van J.; Kort, H.S.M.; Markopoulos, P.; Soede, M.

    2007-01-01

    Networked and ubiquitous information and communication technologies (ICTs) and ambient intelligence are increasingly used in the home environment to facilitate independent living for older adults. These systems collect and disperse a high volume of personal data, which is used for assistance and

  5. Construindo cidadania ambiental na escola

    Directory of Open Access Journals (Sweden)

    Cibele Schwanke

    2014-03-01

    Full Text Available http://dx.doi.org/10.5007/1807-0221.2013v10n16p14 O forte componente transversal da Educação Ambiental possibilita sua inserção em vários espaços e níveis de escolaridade. No ensino fundamental, constitui-se em uma importante ferramenta para criar espaços que permitam a abordagem de temáticas socioambientais atuais, de forma crítica e participativa. O presente trabalho tem como objetivo apresentar o Projeto Construindo Cidadania Ambiental, executado por bolsistas do Grupo PET - Conexões Gestão Ambiental em unidades escolares, explicitando sua filosofia de implantação e resultados obtidos até o momento. Verifica-se que sua natureza interdisciplinar e integrada permite uma efetiva interação com o corpo docente e discente da escola, bem como permite a prática de uma educação ambiental crítica e transformadora.

  6. SELEKSI DAN IDENTIFIKASI BAKTERI ENDOFIT POTENSIAL PENGHASIL ENZIM PROTEASE DARI TAMAN NASIONAL GUNUNG HALIMUN - (The Selection and Identification of Potential Endophyte Bacteria as Protease Enzyme Producer from Halimun Mount National Park

    Directory of Open Access Journals (Sweden)

    Ruth Melliawati

    2016-12-01

    Full Text Available Endophytic bacteria have an equal chance to bacteria that live outside the plant tissue as potential bacteria. The selection has done towards 326 bacterial endophyte isolates. This research aimed to find and identify proteolytic potential isolates. The proteolytic selection of endophytic bacteria had done using solid skim milk. The capability of endophytic bacteria to agglomerate milk was tested using liquid skim milk which incubated for 7 days at room temperature. Enzyme production of four selected isolates was made through fermentation in GYS medium. The results showed that 86 isolates have proteolytic potential. Isolate HL.29B.63 had highest protease enzymes activity (65.918 U/mL. Medium optimization was able to increase the enzyme activity into 89.94% (125.04 U/mL. The analysis used 16s rDNA showed that isolate HL.29B.63 was Bacillus amyloliquefacient subs. plantarum strain FZB42.Keywords: endophytic bacteria, fermentation, identification, protease, selection ABSTRAKBakteri endofit mempunyai peluang yang sama dengan bakteri yang hidup diluar jaringan tanaman sebagai bakteri potensial. Seleksi dilakukan terhadap 326 isolat bakteri endofit. Tujuan penelitian ini adalah mencari isolat yang berpotensi proteolitik dan mengidentifikasinya. Seleksi proteolitik terhadap bakteri endofitik menggunakan skim milk padat. Uji kemampuan bakteri endofitik dalam menggumpalkan susu menggunakan medium skim milk cair yang diinkubasi selama 7 hari pada suhu ruang. Produksi enzim terhadap empat isolat terseleksi dilakukan melalui fermentasi dalam medium GYS. Hasilnya menunjukkan bahwa 86 isolat mempunyai potensi proteolitik. Isolat HL.29B.63 mempunyai aktif enzim protease tertinggi (65,918 U/mL. Optimasi medium dapat meningkatkan aktivitas enzim sebesar 89,94% (125,04 U/mL. Analisis menggunakan 16s rDNA menunjukkan bahwa isolat HL.29B.63 adalah Bacillus amyloliquefaciens subs. plantarum strain FZB42.Kata kunci: bakteri endofit, fermentasi, identifikasi, protease

  7. Caracterización de la actividad enzimática y polimorfismos genéticos de butirilcolinesterasa (BCHE) en una población de trabajadores expuestos a plaguicidas inhibidores de colinesterasa en el municipio de Soacha 2014

    OpenAIRE

    Castiblanco Rodriguez, Ana Lucia

    2014-01-01

    Los plaguicidas inhibidores de colinesterasa son sustancias capaces de inhibir a las enzimas acetil y butirilcolinesterasa, impidiendo que se degrade la acetilcolina, lo que produce un aumento en su concentración y, por tanto, una prolongación de los efectos del neurotransmisor. En este estudio se evaluó la actividad enzimática de colinesterasa plasmática, seudocolinesterasa o butirilcolinesterasa (BChE), en dos poblaciones, una expuesta a plaguicidas inhibidores de colinesterasa y un grup...

  8. Avaliação da influência da temperatura e do tratamento enzimático no comportamento reológico do suco de abacaxi pérola (Ananas Comosus L. merr.

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    Adriano Cesar Calandrini Braga

    2013-03-01

    Full Text Available O objetivo deste trabalho foi determinar o comportamento reológico do suco de abacaxi- pérola natural e tratado com enzimas pectinolíticas. As condições de tratamento enzimático foram otimizadas através de um planejamento experimental do tipo fatorial completo 2k, com três repetições do ponto central. Na avaliação do comportamento reológico foram utilizadas duas amostras submetidas a peneiramento (N e D, analisadas em quatro diferentes temperaturas (10; 25; 50 e 65 °C. As análises reológicas foram realizadas utilizando um viscosímetro de cilindros concêntricos Brookfield e os dados experimentais foram ajustados ao modelo de Mizrahi-Berk. A relação entre temperatura e viscosidade aparente foi descrita por uma equação tipo Arrhenius. A otimização da atividade enzimática indicou, através da análise de variância e da metodologia de superfície de resposta, que as variáveis temperatura e tempo de tratamento exerceram efeito estatisticamente significativo (p<0,05 sobre a concentração de pectina presente na amostra. O modelo utilizado mostrou-se adequado para descrever o comportamento reológico dos sucos de acordo com os parâmetros R², χ² e Bf. Os baixos valores obtidos para o índice de comportamento indicaram um comportamento pseudoplástico (n<1. A temperatura exerceu influência sobre a tensão de cisalhamento e a viscosidade aparente dos sucos analisados, sendo os menores valores observados nas amostras analisadas a 65 °C. A equação tipo Arrhenius descreveu de modo satisfatório o efeito da temperatura sobre a viscosidade aparente. Os valores da energia de ativação (Eat foram de 4,54 Kcal.g.mol-1 e 4,89 Kcal.g.mol-1, respectivamente, para as amostras do suco de abacaxi natural e despectinizado, aumentando com o tratamento enzimático. A atividade enzimática proporcionou uma redução nos valores dos parâmetros de comportamento reológico das amostras, bem como na viscosidade aparente, em todas as

  9. IC design challenges for ambient intelligence

    NARCIS (Netherlands)

    Aarts, E.H.L.; Roovers, R.L.J.

    2003-01-01

    The vision of ambient intelligence opens a world of unprecedented experiences: the interaction of people with electronic devices is changed as contextual awareness, natural interfaces and ubiquitous availability of information are realized. We analyze the consequences of the ambient intelligence

  10. Historical Ambient Air Quality Data Inventory

    Data.gov (United States)

    U.S. Environmental Protection Agency — The Historical Ambient Air Quality Data Inventory contains measured and estimated data on ambient air pollution for use in assessing air quality, assisting in...

  11. Feral pigeons in urban environments: dietary flexibility and enzymatic digestion? Palomas domésticas en ambientes urbanos: ¿flexibilidad dietaria y digestión enzimática?

    Directory of Open Access Journals (Sweden)

    MARÍA EUGENIA CIMINARI

    2005-06-01

    Full Text Available Columba livia, original from Europe, is at present widely distributed all over the world. These granivores colonized urban environments where the availability of crops and seeds is not always permanent and, for that are forced to exploit other resources with different composition, e.g. high protein foodstuff. Thus, feral pigeons should have the ability to survive on a diet rich in protein as they do with starchy items by having an adequate digestive biochemical machinery to process it. Phylogenetical and functional hypothesis has been proposed linking dietary flexibility and enzyme lability. All Passeriformes studied to date show the expected positive correlation between aminopeptidase-N and dietary protein but not for intestinal carbohydrases. Conversely, all the non-passerine species modulate intestinal carbohydrases, but not peptidases. Moreover, different scenarios may be posed as the output of a phylogenetical effect, e.g., adding constraints to a lability scheme in certain groups or just determining it (e.g., intestinal disaccharidases modulated in Galloanserae and peptidases modulated in Passeriformes. Consequently, we tested the prediction that feral pigeons adjust digestive enzyme activities according to the level of the respective substrate (e.g., carbohydrates, protein in the diet. Birds were fed for 15 days with two different diets, one with high protein content (low in starch (HP and the other rich in starch and low in proteins (HS. Pigeons fed on the HP were able to survive with no other dietary supplement, as predicted. Pancreatic enzymes did not change between diet treatments. Birds fed on HP exhibited the predicted upward modulation of aminopeptidase-N activity, when compared to birds on HS, while intestinal carbohydrases did not show differences between diets. These results give an apparent support to the functional hypothesis, but are not enough to reject that the observed intestinal protease lability has a phylogenetical component, because feral pigeons are closely related to Passeriformes. Finally, feral pigeons have the ability to take full advantage of the energy content from proteins, having enough pancreatic proteases activity and modulating the intestinal aminopeptidase-N. These abilities may allow them to use effectively the nutrients found in the cities surviving even when grains are not available along the yearLa paloma doméstica, originaria de Europa, está distribuida alrededor del mundo. Estas aves granívoras colonizan zonas urbanas donde granos y semillas no están disponibles todo el año, por lo que deben explotar recursos con una composición química diferente, (e.g., ricos en proteínas. Entonces, estas aves debieran poseer la capacidad de sobrevivir con una dieta proteica, utilizando una maquinaria bioquímica digestiva adecuada. Se han propuesto dos hipótesis, una funcional y otra filogenética, para explicar la flexibilidad dietaria y la labilidad de las enzimas intestinales en aves. Los Paseriformes estudiados al momento muestran una correlación positiva entre N-aminopeptidasa y las proteínas de la dieta, aunque esto no es aparente para las carbohidrasas intestinales. Por el contrario, todas las especies no-paseriformes estudiadas modulan las carbohidrasas intestinales, pero no las peptidasas. A su vez se pueden representar diferentes escenarios como producto de la filogenia, por ejemplo, agregando limitaciones a patrones de labilidad o directamente determinándolos (e.g., la modulación de las disacaridasas en Galloanserae y la modulación de las peptidasas en Paseriformes. Por esto, sometimos a prueba que las palomas ajustan la actividad de las enzimas digestivas de acuerdo al nivel de su respectivo sustrato (carbohidratos o proteínas en la dieta. Dos grupos de palomas fueron alimentadas 15 días con dietas diferentes, una rica en proteínas y pobre en almidón (HP y otra rica en almidón y pobre en proteínas (HS. Las palomas alimentadas con HP sobrevivieron sin suplementos dietarios adicionales. Las enzimas pancreáticas fueron iguales entre tratamientos. Las palomas alimentadas con HP exhibieron una actividad de N-aminopeptidasa mayor que la de HS, mientras que las carbohidrasas fueron iguales entre dietas. Estos resultados dan soporte a la hipótesis funcional, pero también, son insuficientes para rechazar que la labilidad de la proteasa intestinal constituye un efecto filogenético, ya que estas aves constituyen un grupo cercano a los Passeriformes. En síntesis, las palomas poseen la capacidad de utilizar las proteínas debido a que poseen una actividad proteásica pancreática adecuada y modulan la N-aminopeptidasa. Estas características permiten que las palomas domésticas aprovechen efectivamente alimentos que ofrecen las ciudades y sobrevivan aun cuando la disponibilidad de granos sea escasa

  12. Tipologia para a contabilidade ambiental

    Directory of Open Access Journals (Sweden)

    Nazlhe Faride Chein Schekaiban

    2007-06-01

    Full Text Available Este artigo revê a visão, as propostas e o desenvolvimento da contabilidade ambiental, refletindo sobre suas implicações, com a finalidade de descobrir e encontrar sua importância e situação. Para se chegar a esse resultado foi preciso realizar uma revisão epistemológica moldada e processo reflexivo de sustentabilidade e da aproximação ao usuário, da percepção da realidade contábil no México e da gerência interna das organizações. As conclusões mostram a contabilidade ambiental no México fora da re-alidade operativa do modelo contábil regional, aumentando a importância de se criar uma cultura capaz de examinar o controle da missão deste tipo de contabilidade.

  13. Responsabilidades municipales en materia ambiental

    Directory of Open Access Journals (Sweden)

    Ignacio Pichardo Pagaza

    2009-01-01

    Este trabajo reflexiona en torno a las responsabilidades que la Constitución política impone de manera exclusiva a los municipios y que por su naturaleza tienen efectos directos en el medio ambiente. Se alude aquí a los servicios de agua po ta ble, drenaje, saneamiento, tratamiento de aguas residuales, disposición de residuos sólidos, rastros, panteones y mercados. Ahora son los desafíos ambientales de la autoridad municipal, por lo que deben ser también sus prioridades. Si esos servicios no se atienden oportuna y técnicamente la población sufrirá, se deteriorará gravemente el medio ambiente y disminuirá la calidad de la vida de la comunidad.

  14. Ambiente psicologico en las organizaciones

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    Damarcy Cortés Baracaldo

    2002-01-01

    Full Text Available El talento humano en las organizaciones se ha convertido en las ultimas decadas en un recurso que se administra de acuerdo al estilo de liderazgo del jefe, lo que implica una marcada relación hacia la tarea, hacia las relaciones con el personal o una combinación de estas dos, que desencadenan en un ambiente psicológico exclusive en cada organización.

  15. Ambient Volatility of Triethyl Phosphate

    Science.gov (United States)

    2017-08-01

    of materials is predictable using Raoult’s law. This report details the measurement of the effect of water vapor partial pressure on the volatility...empirical correlation taking into account nonideal behavior was developed to enable estimation of TEPO volatility at any combination of ambient...of the second component is expected to be one-half as much as in the absence of water vapor. Similarly, the measured volatility of the second

  16. Haciendas Locales y Medio Ambiente

    OpenAIRE

    Rozas Valdés, José Andrés

    1997-01-01

    Junto a los medios puramente administrativos orientados a la protección del medio ambiente, cada día adquieren mayor protagonismo los que pueden adoptarse desde el ámbito del derecho financiero, del ingreso y gasto públicos. El trabajo se ha estructurado en cuatro apartados: aguas, residuos sólidos, polución atmosférica y contaminación acústica.

  17. Evaluación de pretratamientos químicos para la hidrólisis enzimática de residuos lignocelulósicos de yuca (Manihot esculenta Crantz

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    Lilibeth Niño López

    2013-01-01

    Full Text Available El efecto de diferentes pretratamientos químicos con ácido sulfúrico (H2SO4, hidróxido de sodio (NaOH y peróxido de hidrogeno (H2O2, fueron evaluados mediante la cuantificación de azucares reductores totales producidos durante la etapa de hidrólisis enzimática de los residuos lignocelulósicos de yuca (Manihot esculenta Crantz y el porcentaje (% de remoción de lignina después del pretratamiento. La cantidad de residuos utilizados (1 y 5% (w/v a diferentes tamaños de partícula (1,18 y 0,6mm fueron pretratados por separado (hojas y tallos con H2SO4, NaOH y H2O2 a diferentes concentraciones (1 y 5% (w/v. Los residuos vegetales fueron hidrolizados con la enzima accellerase 1500 a 50°C, pH 5 y 140 rpm. El pretratamiento con NaOH permite la mayor liberación de azucares reductores totales durante la hidrólisis enzimática (3,7g/L en hojas seguido del pretratamiento con H2SO4 (2,11g/L y H2O2 (1,54g/L. En tallos las concentraciones de azúcares son menores.

  18. Caracterização de cepas de plasmodium falciparum do Estado de Rondônia, Brasil, utilizando microtestes de sensibilidade aos antimaláricos, tipificação enzimática e anticorpos monoclonais

    Directory of Open Access Journals (Sweden)

    S. M. Di Santi

    1987-06-01

    Full Text Available Nove amostras de Plasmodium falciparum foram coletadas de migrantes infectados no Estado de Rondônia. Estas amostras foram mantidas em cultivo para caracterização por tipificação enzimática em acetato de celulose, sensibilidade á cloroquina, amodiaquina, mefloquina e quinino e análise da diversidade antigê-nica através de anticorpos monoclonais específicos. Os resultados obtidos mostraram variação entre todas as amostras estudadas: encontrou-se resistência crescente à cloroquina, resistência intermediária à amodiaquina e quinino e sensibilidade a baixos níveis de mefloquina; apenas dois isolados mostraram sensibilidade a todas as drogas. A tipificação enzimática mostrou presença de parasitas GPI 1 e 2 e ADA 1 e 2, enquanto que para PEP e LDH todas as amostras foram do tipo 1. Sorotipagem com anticorpos monoclonais PSA mostrou presença de três sorotipos diferentes (II, III e IV. Estes resultados mostraram: a variação entre as amostras para os marcadores analisados; b nesta região, para o pequeno número de amostras analisadas, não foram observadas diferenças significativas ou novos tipos de parasitas.

  19. Parâmetros reacionais para a síntese enzimática do butirato de butila em solventes orgânicos Reactional parameters for enzymatic synthesis of butyl butyrate in organic solvent

    Directory of Open Access Journals (Sweden)

    Heizir F. CASTRO

    1997-12-01

    Full Text Available A síntese orgânica catalisada por enzimas envolve um mecanismo complexo dependente do tipo de substrato, enzima, solvente orgânico e teor de água no meio reacional. Neste trabalho foi estudado a influência de alguns desses parâmetros no rendimento da esterificação do butanol com ácido butírico, utilizando uma preparação enzimática comercial de lipase. A polaridade e natureza do solvente, bem como a razão molar entre o butanol e ácido butírico, foram considerados os fatores que mais influenciaram o desenvolvimento dessa síntese enzimática.The organic synthesis catalyzed by enzymes is a complex function of substrate concentration, water concentration in the liquid phase, enzyme and organic solvent properties. In this work the influence of some parameters on the esterification of butanol with butyric acid was investigated, using a commercial lipase preparation. The polarity and nature of the solvent and also the substrate mole ratios played an important role in the performance of this enzymatic synthesis.

  20. Karakterisasi stem cell pulpa gigi sulung dengan modifikasi enzim tripsin (The characterization of stem cells from human exfoliated deciduous teeth using trypsin enzym

    Directory of Open Access Journals (Sweden)

    Tri Wijayanti Puspitasari

    2014-06-01

    , Rumah Sakit Gigi dan Mulut Universitas Airlangga yang telah memenuhi kriteria. Pulpa gigi diisolasi dan dikultur dengan modifikasi enzim Trypsin. Hasil: SHED yang sehat hasil dari modifikasi teknik isolasi dan kultur positif menunjukkan ekspresi marker CD105 dengan berfluoresensi berwarna hijau dilihat melalui mikroskop fluoresen. Simpulan: SHED yang dikultur dan diisolasi dengan teknik modifikasi positif dikarakterisasi dengan marker CD105.

  1. Hidrólisis enzimática de residuos de la cosecha de caña de azúcar

    Directory of Open Access Journals (Sweden)

    Jairo G. Salcedo Mendoza

    2012-01-01

    Full Text Available Título en inglés: Hydrolysis Enzymatic of crop residues sugar cane Resumen: En esta investigación, se hidrolizó un sustrato deslignificado proveniente de residuos de la cosecha caña de azúcar  (hojas y cogollos usando un  preparado enzimático con 27.53 unidades de papel filtro (FPU, obtenido a partir de enzimas comerciales. La hidrólisis se llevó a cabo a un pH de 4.2 y una temperatura de 50 oC. Fueron analizados  modelos de inhibición por sustrato, glucosa e inhibición total por producto. Los resultados mostraron que los modelos que mejor se ajustan a los datos experimentales, son los modelos de inhibición competitiva por glucosa, con una constante de Michaelis (Km de 20.37 g/L,  velocidad máxima (Vmax 39 g/L h y una constante de inhibición  (ki de 0.442. En el caso que las relaciones  enzima – Sustrato (E/S sean mayores  de 0.5, se puede aplicar el modelo cinético de Michaelis-Menten. Palabras clave: Inhibición; modelos cinéticos; hojas y cogollos; coctel de enzimas Abstract: In this research, a delignified substrate from crops residues sugar cane residues (leaves and top cane was hydrolyzed using an enzyme preparation with 27.53 FPU. This enzyme was obtained from trade. Hydrolysis was carried out to pH of 4.2 and a temperature of 50 oC. Models of inhibition models substrate, glucose and total inhibition product was analyzed. The results showed that models that best fit the data experimental was the models competitive glucose inhibition (Km= 20.37, Vmax=39 and ki= 0.442. In the event that E/S is above 0.5, can applied kinetic models of Michaelis – Menten. Keywords: Inhibition; kinetic models; leaves and tops cane; enzyme cocktail.

  2. Alterações pós-colheita da "fruta-de-lobo" (Solanum lycocarpum St. Hil. durante o amadurecimento: análises físico-químicas, químicas e enzimáticas Postharvest changes of "fruta-de-lobo" (Solanum lycocarpum St. Hil. during the ripening process: physical-chemical, chemical and enzymatic analysis

    Directory of Open Access Journals (Sweden)

    Enio Nazaré de Oliveira Junior

    2004-12-01

    Full Text Available Estudaram-se, neste trabalho, algumas modificações físico-químicas, químicas e enzimáticas, em pós-colheita, durante o amadurecimento da fruta-de-lobo, comparando-as com as de outros frutos. Os frutos colhidos de plantas nativas no início do estádio de amadurecimento foram selecionados e armazenados durante 18 dias à temperatura ambiente (17,1 a 26,9ºC. Os frutos foram lavados com água destilada, descascados, picados, congelados em nitrogênio líquido e liofilizados até massa constante. O delineamento experimental utilizado foi o inteiramente casualizado, com quatro repetições de 3 frutos. Os valores de pH e acidez titulável não variaram estatisticamente durante o amadurecimento. A atividade de amilase e os teores de amido diminuíram gradativamente com o aumento dos açúcares solúveis totais e não redutores, enquanto os teores dos redutores se mantiveram constantes. Os teores de pectinas totais e solúveis diminuíram, enquanto a atividade de pectinametilesterase manteve-se constante. As atividades das enzimas poligalacturonase e polimetilgalacturonase não foram detectadas no fruto. Observou-se diminuição dos teores de polifenóis e das atividades das enzimas peroxidase e polifenoloxidase e conseqüente diminuição do escurecimento da polpa do fruto.Physical-chemical, chemical and enzymatic modifications were studied during the ripening of "fruta-de-lobo" compared with those in other fruits. The fruits were harvested from native plants in the ripening initial stage and they were selected and stored during 18 days at room temperature (17.1 to 26.9ºC. The fruits were washed with distilled water and peeled. Their pulps were chopped up, frozen in liquid nitrogen and lyophilized until they get a constant mass. The pH and tritable acidity did not show statistical variation during the ripening process. The amylase activity and the starch contents decreased gradually and the soluble total sugar and nonreducing sugar increased

  3. Behaviour of enzymatic activities and root elongation in Argiudoll soils from the Argentine Humid Pampa treated with biosolids Comportamiento de actividades enzimáticas y elongación de raíces en suelos Argiudoles de la Pampa Húmeda, Argentina, tratados con biosólidos

    Directory of Open Access Journals (Sweden)

    E.B.R. Perotti

    2008-06-01

    Full Text Available The incorporation of biosolids to soil is a strategy aiming at the re-location of these materials in the environment with a useful end: soil fertilization. In this work, the response of two Argiudoll soils (one with more than 100 years of agriculture and the other, a virgin one to biosolid incorporation was studied under laboratory conditions. To measure this response, soil enzymatic biodescriptors, such as dehydrogenase and urease activities, and tests related to plant physiology (the root elongation test were employed. The addition of the biosolid to both soils had a stimulating effect though different on each soil according to the added dose. Adjustment of the regression line for dehydrogenase activity with root elongation was positive and statistically significant (pLa incorporación de biosólidos al suelo es una estrategia que tiene como objetivo la reubicación de estos materiales en el ambiente con un fin útil, como es la fertilización del suelo. En este trabajo se estudió, en condiciones controladas de laboratorio, la respuesta de dos suelos Argiudoles (uno con más de 100 años de agricultura y otro virgen frente a la perturbación físico-química y biótica que genera la incorporación de un biosólido. Para medir esta respuesta se emplearon dos biodescriptores edáficos (las actividades deshidrogenasa y ureasa y un tercero referido a la fisiología vegetal, la prueba de elongación de raíces. La incorporación del biosólido en ambos suelos, en general no deprimió el funcionamiento de las actividades enzimáticas estudiadas; contrariamente, según la dosis aportada tuvo un efecto estimulante, aunque diferente, entre ambos suelos. El ajuste de la recta de regresión de la actividad deshidrogenasa con la elongación de las plántulas fue positivo y altamente significativo, lo que indica la complementaridad de ambos descriptores. Los resultados obtenidos sugieren que los biodescriptores empleados resultaron aptos para estudiar el

  4. Maquinas virtuais em ambientes seguros

    OpenAIRE

    Arthur Bispo de Castro

    2006-01-01

    Resumo: Desde o início da computação a idéia de máquinas virtuais vem sendo aplicada para estender o multiprocessamento, multi-programação e multi-acesso, tornando os sistemas multi-ambiente. O contínuo aumento no poder de processamento dos computadores fez com que máquinas muito rápidas estivessem ao alcance de qualquer usuário, surgindo PCs com processamento, espaço em disco e memória suficiente para comportar mais de um sistema compartilhando o mesmo hardware. Basicamente, o objetivo das m...

  5. educación ambiental

    Directory of Open Access Journals (Sweden)

    Fernando Ojeda Barceló

    2008-01-01

    Full Text Available Las Tecnologías de la Información y Comunicación (TICs pueden constituir una herramienta de primer orden para la Educación Ambiental para la Sostenibilidad (EApS, pero todavía existe cierta reticencia por parte de educadores ambientales a hacer un uso de ellas de forma habitual. El objetivo fundamental de este trabajo es ofrecer una revisión del estado de la cuestión tanto a nivel nacional como internacional e intentar hacer una propuesta didáctica de trabajo colaborativo a través de Internet para estudiantes de secundaria.

  6. Pediatria ambiental: um tema emergente

    Directory of Open Access Journals (Sweden)

    Patricia M. Valenzuela

    2011-04-01

    Full Text Available OBJETIVO: Revisar os artigos mais relevantes sobre a pediatria ambiental, seus efeitos potenciais para a saúde e, especialmente, seus avanços na prevenção. FONTES DOS DADOS: Foi realizada uma pesquisa utilizando as bases de dados MEDLINE/PubMed e SciELO. Foram revisados artigos de 1990 a 2010, além de capítulos de livros relacionados à pediatria ambiental. SÍNTESE DOS DADOS: Há uma variedade significativa de fatores que tornam as crianças altamente vulneráveis à exposição a riscos ambientais, associados principalmente ao consumo comparativamente maior de água, comida e ar por parte da criança, em relação ao seu peso corporal. De acordo com a Organização Mundial de Saúde, mais de 3 milhões de crianças menores de 5 anos morrem devido a doenças relacionadas ao meio ambiente. Aproximadamente 30-40% das doenças pediátricas estão relacionadas a fatores ambientais. As crianças estão constantemente expostas a vários riscos ambientais para a saúde, dentre os quais se destacam: água contaminada, falta de condições adequadas de saneamento, poluição do ar, vetores de doenças, perigos químicos, injúrias e acidentes. CONCLUSÕES: Atualmente, os pediatras são desafiados a tratar das necessidades de saúde ligadas à pediatria ambiental. A história pediátrica deve ser mais abrangente, acrescentando-se questões pontuais que ajudem a identificar potenciais riscos ambientais. A conscientização e o entendimento sobre os efeitos nocivos das várias condições ambientais e o conhecimento sobre as medidas de prevenção relacionadas resultarão em intervenções oportunas e adequadas que melhorarão a saúde e o desenvolvimento das nossas crianças.

  7. Atividade da colinesterase plasmática como biomarcador de impacto ambiental em tartarugas verdes (Chelonia mydas no litoral do Arquipélago de Fernando de Noronha, Pernambuco

    Directory of Open Access Journals (Sweden)

    Leandro A. Fonseca

    2015-04-01

    Full Text Available Biomarcadores podem ser usados de forma preditiva, permitindo que sejam tomadas ações de controle antes que ocorram danos ambientais irreversíveis com consequências ecológicas severas, no entanto, espécies sentinelas são necessárias para avaliação desses marcadores. As tartarugas marinhas são consideradas espécies sentinelas quando acometidas por fibropapilomas, sendo sinalizadora do desequilíbrio ambiental marinho nas suas áreas de ocorrência. Com o objetivo de propor a determinação da atividade da colinesterase plasmática em tartarugas verdes (Chelonia mydas como biomarcador, procedeu-se a determinação da atividade enzimática em animais saudáveis e em localidade de baixo impacto antrópico (Arquipélago de Fernando de Noronha, Pernambuco, Brasil para servir como referência para comparação com animais capturados em locais de maior impacto antrópico. Ao todo foram analisadas amostras de plasma heparinizado de 35 animais capturados. Todas as amostras analisadas apresentaram alguma atividade enzimática de colinesterase plasmática. Os valores obtidos de colinesterase variaram de 162 a 379 UI/L, com média e desvio padrão de 216,4 ± 51,4 UI/L. Nos estudos de repetibilidade e reprodutibilidade obtiveram-se coeficientes de variação menor que 5% em todas as análises, portanto a metodologia analítica utilizada se mostrou confiável. A longevidade das tartarugas marinhas da espécie C. mydas, o comportamento alimentar, juntamente com o fato de possuirem atividade enzimática detectável podem indicar essa espécie como bioindicadora de exposição a poluentes que influenciam na atividade da colinesterase plasmática

  8. Turbine airfoil with ambient cooling system

    Science.gov (United States)

    Campbell, Jr, Christian X.; Marra, John J.; Marsh, Jan H.

    2016-06-07

    A turbine airfoil usable in a turbine engine and having at least one ambient air cooling system is disclosed. At least a portion of the cooling system may include one or more cooling channels configured to receive ambient air at about atmospheric pressure. The ambient air cooling system may have a tip static pressure to ambient pressure ratio of at least 0.5, and in at least one embodiment, may include a tip static pressure to ambient pressure ratio of between about 0.5 and about 3.0. The cooling system may also be configured such that an under root slot chamber in the root is large to minimize supply air velocity. One or more cooling channels of the ambient air cooling system may terminate at an outlet at the tip such that the outlet is aligned with inner surfaces forming the at least one cooling channel in the airfoil to facilitate high mass flow.

  9. Níveis de óleo e adição de complexo enzimático na ração de frangos de corte

    Directory of Open Access Journals (Sweden)

    Pucci Luiz Eduardo Avelar

    2003-01-01

    Full Text Available Dois experimentos foram conduzidos para avaliar o efeito da adição de óleo de soja e de um complexo enzimático em rações à base de milho e farelo de soja, sobre o desempenho de frangos de corte e a digestibilidade dos nutrientes. No experimento 1, foram utilizados 960 pintos de corte, não sexados, os quais receberam os tratamentos em esquema fatorial 4 x 2 (quatro níveis de inclusão de óleo de soja: 0; 2,5; 5,0 e 7,5%, com ou sem adição de um complexo enzimático contendo amilase, xilanase e protease, em quatro repetições de 30 aves cada, no período de 1 a 21 dias de idade. Dos 22 aos 42 dias de idade, todas as aves receberam uma mesma ração de crescimento, para avaliação de possível efeito residual dos tratamentos aplicados na fase inicial de criação sobre a fase seguinte. Aos 21 dias de idade, 90 aves (três machos e duas fêmeas de cada unidade experimental foram transferidas para uma sala de metabolismo (experimento 2, em que as aves receberam as mesmas rações experimentais, para determinação dos valores energéticos e dos coeficientes de digestibilidade da matéria seca e do extrato etéreo. Não houve efeito da adição do complexo enzimático, nem interação dos níveis de óleo e suplementação com enzimas sobre o desempenho das aves. O valor energético das rações melhorou em 2,3% com a adição de óleo, não havendo efeito da adição de enzimas. O nível de 2,33% de óleo permitiu máxima digestibilidade do extrato etéreo. Concluiu-se que a inclusão de óleo na ração melhorou o desempenho das aves e a conversão alimentar permaneceu constante a partir de 2,18% de inclusão.

  10. Disminución de la necesidad de cobertura con injertos y baja tasa de cicatrización hipertrófica en quemaduras tras desbridamiento enzimático

    Directory of Open Access Journals (Sweden)

    Carlos Corrales-Benítez

    Full Text Available Introducción y Objetivo. El tratamiento clásico de las quemaduras se basa en la eliminación precoz de la escara; sin embargo, el desbridamiento tangencial de las quemaduras mixtas y profundas retira tejidos dérmicos viables, obligando a la cobertura con autoinjertos. El desbridamiento enzimático con enzimas proteolíticos enriquecidos con Bromelaína (Nexobrid® mantiene restos dérmicos que pueden permitir la reepitelización por sí misma de la quemadura. Evaluamos el uso en nuestra unidad de Nexobrid® (MediWound Ltd., Israel en quemaduras mixtas y dérmicas profundas, valorando la capacidad de reepitelización tras su uso, la tasa de injerto en los pacientes tratados, y la aparición de cicatrización hipertrófica. Pacientes y Método: Evaluamos retrospectivamente 36 pacientes tratados con Nexobrid® entre enero de 2015 y febrero de 2016, 11 mujeres y 25 varones con una media de edad de 42.89 años. La superficie corporal quemada media fue del 7.47% y la diagnosticada como profunda del 6.42%.Todos los pacientes presentaron quemaduras profundas con necesidad de cirugía para su desbridamiento y cobertura con injertos. Resultados: Tras el desbridamiento enzimático solo el 36.1% de los pacientes (13 de 36 requirió cobertura con injertos (p < 0.001.La superficie injertada fue tan solo del 2.67% frente al 4.98% que fue desbridada enzimáticamente (p < 0.001. A pesar de que el tiempo hasta la epitelización total de las quemaduras se alargó hasta los 25.69 días, tan solo un 11.1% de los pacientes desarrolló cicatriz hipertrófica. Conclusiones: Nexobrid® permite el desbridamiento completo de las quemaduras mixtas y dérmicas profundas disminuyendo el porcentaje de pacientes que requieren autoinjertos y la superficie injertada, con bajas tasas de cicatrización hipertrófica.

  11. Use of Enzymatic Biosensors as Quality Indices: A Synopsis of Present and Future Trends in The Food Industry Uso de Biosensores Enzimáticos como Indicadores de Calidad: Una Sinopsis del Presente y Futuro en la Industria Alimentaria

    Directory of Open Access Journals (Sweden)

    Liliana Serna Cock

    2009-06-01

    Full Text Available Biosensors are an important alternative in the food industry to ensure the quality and safety of products and process controls with effective, fast and economical methods. Their technology is based on a specific biological recognition element in combination with a transducer for signal processing. The use of enzymatic biosensor technology in food processing, quality control and on-line processes is promising compared to conventional analytical techniques, as it offers great advantages due to size, cost, specificity, fast response, precision and sensitivity. This article reviews the development and use of some enzyme biosensors in the food industry, describes the most important application areas and analyzes the current situation and future possibilities. In conclusion, enzymatic biosensors are a tool with broad application in the development of quality systems, risk analysis and critical control points, and the extent of their use in the food industry is still largely limited by the short lifetime of biosensors, in response to which the use of thermophilic enzymes has been proposed.Los biosensores constituyen una importante alternativa en la industria de alimentos para garantizar la calidad e inocuidad de los productos y controlar los procesos con métodos eficaces, rápidos y económicos; su tecnología está basada en un elemento de reconocimiento biológico específico en combinación con un transductor para el procesamiento de la señal. El uso de técnicas de biosensores enzimáticos en procesamiento de alimentos, control de calidad y de procesos “on line”, es prometedor frente a las técnicas analíticas convencionales, ya que ofrecen grandes ventajas debido a su tamaño, costo, especificidad, respuesta rápida, precisión y sensibilidad. En este artículo se revisa el desarrollo y uso de algunos biosensores enzimáticos en la industria alimentaria, se describen las áreas de aplicación más importantes y se analiza su situaci

  12. Tıbbi İlaçlar ve Kişisel Bakım Ürünlerinin (PPCPs Triticum aestivum L. Üzerinde Antioksidan Enzim Aktivitelerine Etkileri

    Directory of Open Access Journals (Sweden)

    Etem OSMA

    2017-01-01

    Full Text Available Bu çalışma tıbbi ilaç ve kişisel bakım ürünlerinin ekmeklik buğday (Triticum aestivum L. cv. Bezostaja bitkisi üzerindeki antioksidan enzim aktiviteleri hakkındadır. Araştırmada günlük hayatta sıklıkla tüketilen Acetaminophen, Caffein, Gemfibrozil ve β-estradiol olmak üzere dört farklı tıbbi ilaç etken maddesi kullanılmıştır. Ekim yapılacak toprağa 15 mg, 50 mg, 200 mg Acetaminophen, Gemfibrozil ve β-estradiol ile bunun yanı sıra 50 mg, 100 mg, 300 mg şeklinde farklı konsantrasyonlarda Caffein karıştırılmıştır. Daha sonra, hazırlanan 650 g toprağın üzerine 7 g buğday tohumu ekilmiş, bunun üzeri ise 100 g toprak ile kaplanmıştır. Çimlenen buğdaylar, 15 gün sonra hasat edilmiştir. Hasat edilen örneklere ekstraksiyon işlemi uygulandıktan sonra, örneklerde katalaz (CAT, peroksidaz (POD, süperoksit dismutaz (SOD gibi antioksidan enzim aktiviteleri belirlenmiştir. Elde edilen sonuçlar incelendiğinde buğdayda; CAT değerlerinin 1.525-2.350 EU g¯¹ FW, POD değerlerinin 37.200-59.600EU g¯¹ FW, SOD değerlerinin de 38-52,8 EU g¯¹ FW arasında olduğu tespit edilmiştir. Kontrol örnekleri ile farklı konsantrasyonlarda yetiştirilen örnekler arasındaki ilişki %95 güven aralığında istatistiksel olarak değerlendirilmiş, anlamlı farklılıklar belirlenmiştir. Çalışmada, Acetaminophen, Caffein, Gemfibrozil ve β-estradiol uygulanan örneklerde konsantrasyon artışına bağlı olarak kontrol grubuna kıyasla antioksidan enzim aktivitelerinde artış olduğu sonucuna ulaşılmıştır.

  13. Tratamiento enzimático en la extracción de aceite y obtención de antioxidantes a partir de semilla de onagra, Oenothera biennis, por prensado en frío

    Directory of Open Access Journals (Sweden)

    Zúñiga, María E.

    2007-03-01

    Full Text Available The aim of this work was to improve the yield in evening primrose oil extraction and the antioxidant potential of the residual meal using an enzyme assisted extraction process with cold pressing. Among four commercial enzymes, a mixture of two of them was selected in order to evaluate the effect of enzyme-substrate ratio, temperature, moisture and period of time of the enzymatic pretreatment on the extracted oil. The oil yield increased by 12% when the seeds were pretreated with 2% enzyme-substrate ratio of a mixture of 100G Ultrazym and Cellubrix (1:1 for 15 h at 40oC and 40% moisture. The defatted meal obtained from the enzyme aided process enhanced its phenol content and improved its potential antioxidant activity in comparison to the control from 250 to 276 mg of phenols/g of meal and from 1463 to 1558 mg of trolox/g of meal respectively.Se espera que el tratamiento enzimático, previo al prensado en frío, de la semilla de Oenothera biennis, mejore el rendimiento de extracción de aceite y la cantidad de antioxidantes extraídos de la harina residual. De una serie de cuatro preparados enzimáticos comerciales, se selecciona el más adecuado, el cual es evaluado bajo distintas condiciones de incubación: razones enzima/sustrato, tiempo, humedad y temperatura. El rendimiento aumenta un 12 % cuando se realiza un doble prensado sobre la semilla tratada con una mezcla enzimática formulada con Ultrazym 100G y Cellubrix en razón 1:1, concentración enzima/sustrato del 2 % p/p (base húmeda, durante 15 horas de incubación a 45 °C y 40 % de humedad. La harina residual desgrasada aumenta su contenido de antioxidantes desde 250 a 276 mg de fenoles expresados en catequina y el extracto mejora su actividad antioxidante desde 1463 a 1558 mg de trolox por gr de harina.

  14. Influencia del proceso de fibrilación y desfibrilación enzimática en las propiedades mecánicas de hilos de fibras celulósicas regeneradas obtenidas por el proceso NMMO

    Directory of Open Access Journals (Sweden)

    Carrillo, F.

    2001-04-01

    Full Text Available Fibre spinning from a solution of cellulose in N-methyl-morpholine-N-oxidehydrate is an environmentally friendly process (NMMO, which yields cellulose fibres with excellent mechanical properties. A special feature of these fibbers is their ability to fibrillate in the swollen state. It is possible to control and exploit fibrillation of this kind of fibre (generic name: lyocell through mechanical fibrillation and enzymatic defibrillation treatments. The mechanical properties (elongation at break. Young's modulus, tensile strenght and tenacity of solvent (NMMO-spun lyocell yarns samples have been studied in untreated condition as well as after industrial mechanical fibrillation and enzymatic defibrillation treatments. It is observed that the tenacity of lyocell yarn decreases greatly as a result of the treatments, particularly after the enzymatic treatment. For this reason, the control of these operations plays a critical role in ensuring acceptable mechanical properties of the yarns.

    La obtención de fibras hiladas a partir de una solución de celulosa en N-óxido de N-metilmorfolina hidratado permite obtener fibras de celulosa regenerada, con excelentes propiedades mecánicas por medio de un proceso industrial no contaminante (proceso NMMO. Sin embargo, estas fibras presentan una propiedad especial, su elevada fibrilación, principalmente durante los tratamientos en húmedo, que es posible controlar y explotar mediante las etapas de fibrilación mecánica y desfibrilación enzimática de los productos fabricados. En el presente trabajo se estudiaron las propiedades mecánicas (deformación a la rotura, módulo de Young, resistencia máxima a la tracción y tenacidad de muestras de hilo de esta nueva fibra, evaluando la influencia de los tratamientos industriales de fibrilación mecánica y desfibrilación enzimática. Se observó que la tenacidad y la resistencia a la rotura de las muestras disminuyen significativamente después de estos

  15. Below-Ambient and Cryogenic Thermal Testing

    Science.gov (United States)

    Fesmire, James E.

    2016-01-01

    Thermal insulation systems operating in below-ambient temperature conditions are inherently susceptible to moisture intrusion and vapor drive toward the cold side. The subsequent effects may include condensation, icing, cracking, corrosion, and other problems. Methods and apparatus for real-world thermal performance testing of below-ambient systems have been developed based on cryogenic boiloff calorimetry. New ASTM International standards on cryogenic testing and their extension to future standards for below-ambient testing of pipe insulation are reviewed.

  16. Det ambientes fænomenologi

    DEFF Research Database (Denmark)

    Walther-Hansen, Mads

    2014-01-01

    Det ambiente: sansning, medialisering, omgivelse er et aktuelt og ambitiøst værk. Bogen skildrer hvordan ambiente fænomener har fået en stigende betydning i den moderne verden, og redegør for måden hvorpå det ambiente virker ind på hele vores oplevelseskultur. Det er en levende, uprætentiøs og frem...

  17. Ambient temperature signalling in plants.

    Science.gov (United States)

    Wigge, Philip A

    2013-10-01

    Plants are exposed to daily and seasonal fluctuations in temperature. Within the 'ambient' temperature range (about 12-27°C for Arabidopsis) temperature differences have large effects on plant growth and development, disease resistance pathways and the circadian clock without activating temperature stress pathways. It is this developmental sensing and response to non-stressful temperatures that will be covered in this review. Recent advances have revealed key players in mediating temperature signals. The bHLH transcription factor PHYTOCHROME INTERACTING FACTOR4 (PIF4) has been shown to be a hub for multiple responses to warmer temperature in Arabidopsis, including flowering and hypocotyl elongation. Changes in chromatin state are involved in transmitting temperature signals to the transcriptome. Determining the precise mechanisms of temperature perception represents an exciting goal for the field. Copyright © 2013 Elsevier Ltd. All rights reserved.

  18. Some enzymatic properties of cholesterol oxidase produced by Brevibacterium sp Algumas propriedades enzimáticas da colesterol oxidase produzida por Brevibacterium sp.

    Directory of Open Access Journals (Sweden)

    Terezinha J.G. Salva

    1999-12-01

    Full Text Available In this study we determined some properties of the cholesterol oxidase from a Brevibacterium strain isolated from buffalo's milk and identified the cholesterol degradation products by the bacterial cell. A small fraction of the enzyme synthesized by cells cultured in liquid medium for 7days was released into the medium whereas a larger fraction remained bound to the cell membrane. The extraction of this fraction was efficiently accomplished in 1 mM phosphate buffer, pH 7.0, containing 0.7% Triton X-100. The enzyme stability under freezing and at 45oC was improved by addition of 20% glycerol. The optimum temperature and pH for the enzyme activity were 53°C and 7.5, respectively. The only steroidal product from cholesterol oxidation by the microbial cell and by the crude extract of the membrane-bound enzyme was 4-colesten-3-one. Chromatographic analysis showed that minor no steroidal compounds as well as 4-colesten-3-one found in the reaction media arose during fermentation process and were extracted together with the enzyme in the buffer solution. Cholesterol oxidation by the membrane-bound enzyme was a first order reaction type.Neste trabalho foram definidas algumas propriedades da enzima colesterol oxidase produzida por uma linhagem de Brevibacterium sp. isolada de leite de búfala e foram identificados os compostos resultantes da degradação do colesterol pela bactéria. Uma pequena fração da enzima sintetizada pelas células cultivadas em meio líquido por 7 dias foi liberada no meio de cultura e uma fração maior permaneceu ligada à membrana celular. A extração desta fração foi eficientemente efetuada em tampão fosfato 1mM, pH 7,0, contendo 0,7% de triton X-100. A estabilidade da enzima congelada e a 45oC foi aumentada pela adição de 20% de glicerol. A temperatura ótima para a atividade enzimática esteve ao redor de 53(0C e o pH ótimo esteve ao redor de 7,5. O único produto da degradação do colesterol, causada pela a

  19. Identificação de espécies de citros mediante polimorfismo enzimático Identification of citrus species by means of enzymatic polymorphism

    Directory of Open Access Journals (Sweden)

    Haiko Enok Sawazaki

    1992-01-01

    Full Text Available Estudou-se, mediante polimorfismo enzimático em gel de poliacrilamida, a variabilidade genética das espécies de laranja-doce (Citrus sinensis; laranja-azeda (C. aurantium; tangerinas clementina (C. clementina, sunki (C. sunki, cleópatra (C. reshni e poncã (C. rsticulata; lima-da-pérsia (C. limettioides; limão-galego (C. aurantifolia; limão-cravo (C. limonia e trifoliata (Poncirus trifoliata. Extratos de folhas foram analisados para as isoenzimas de malato deidrogenase (MDH, enzima málica (ME, leucino amino peptidase (LAP, glutamato oxaloacetato transaminase (GOT, fosfoglucoisomerase (PGI, fosfoglucomutase (PGM e isocitrato deidrogenase (IDH. Verificou-se grande variabilidade genética interespecífica, porém nenhuma entre os cultivares de laranja-doce. Foram encontradas algumas aloenzimas, além das referidas pela literatura em gel de amido, como aquelas de uma região próxima ao loco conhecido por Pgm-1, responsável por proteínas monoméricas. Este sistema, denominado PGM, revelou a maior diferenciação entre as espécies, tendo apresentado duas regiões distintas com 9 alelos. No sistema MDH, foram considerados dois locas codificando para proteínas diméricas com 7 alelos; no ME, um loco com 3 alelos; no LAP, possivelmente dois locos responsáveis por proteínas monoméricas com 4 alelos; no GOT, dois focos com 7 alelos; no PGI, um loco com 3 alelos e no IDH, um loco com 4 alelos.The genetic diversity of citrus cultivars was studied by polyacrylamide gel electrophoresis on sweet orange (C. sinensis; tangerines (C. clementine, C. sunki, C. reshni, C. reticulata; Palestine lime (C. Iimettioides; West Indian lime (C. aurantifolia; Rangpur lime (C. limonia, Sour orange (C. aurantium and Poncirus trifoliata. Citrus leaf extracts were analysed for isozymes of malato dehidrogenase (MDH, malic enzyme (ME, leucine aminopeptidase (LAP, glutamate oxaloacetate transaminase (GOT, phosphoglucose isomerase (PGI, phosphoglucose mutase (PGM and

  20. Produção de biodiesel por transesterificação enzimática in situ em solvente orgânico a partir da biomassa de Desmodesmus sp.

    Directory of Open Access Journals (Sweden)

    Danielle Kochenborger John

    2017-07-01

    Full Text Available Um dos materiais alternativos mais investigados atualmente para a produção de biodiesel são as microalgas. O presente trabalho teve como objetivo a avaliação do método de transesterificação enzimática in situ da microalga Desmodesmus sp. em solvente orgânico, acompanhando a reação através do monitoramento on-line com sonda de infravermelho e cromatografia gasosa. Dois ensaios foram realizados em condições distintas para efeito comparativo. Baseado nos resultados obtidos por espectroscopia e cromatografia, bem como nas propriedades calculadas a partir do perfil cromatográfico, pode-se concluir que é possível a produção de biodiesel in situ a partir de biomassa de microalgas, reduzindo a etapa de extração de óleo.

  1. Producción de prebióticos galactooligosacáridos mediante técnicas biotecnológicas y de inmovilización enzimática

    OpenAIRE

    González Delgado, Isabel Mª

    2017-01-01

    Tesis Doctoral leída en la Universidad Rey Juan Carlos de Madrid en 2017. Directores de la Tesis: María José López Muñoz y Gabriel Morales Sánchez La presente Tesis Doctoral está dirigida a la optimización de la producción de prebióticos galactooligosacáridos (GOS) por catálisis enzimática. Hoy en día existe una alta demanda de este tipo de alimentos funcionales, ya que los prebióticos aportan beneficios para la salud del consumidor porque estimulan selectivamente el crecimiento de bacteri...

  2. Polimorfismo enzimático em populações de Melipona quadrifasciata anthidioides Lepeletier (Hymenoptera, Apidae, Meliponinae Enzymatic polymorphism in Melipona quadrifasciata anthidioides Lepeletier populations (Hymenoptera, Apidae, Meliponinae

    Directory of Open Access Journals (Sweden)

    Davi S. Aidar

    2001-06-01

    Full Text Available Them aim scope of this study is to characterize the enzymatic polymorphism found in the Melipona quadrifasciata Lepeletier, 1936 populations from Ribeirão Preto, São Paulo and Espírito Santo, Brazil and its hybrids. Samples from each colony (about 52 were prepared for starch gel electrophoresis in order to investigate the genetic variation of the following enzimes: esterase (EST, isocitrate dehydrogenase (IDH, malic enzyme (ME, phosphoglucomutase (PGM, superoxide desmutase (SOD, α-glycerophosphate dehydrogenase (αPGD, malate dehydrogenase (MDH, leucine aminopeptidase (LAP, hexokinase (HK and phosphoglucose isomerase (PGI. The analysis showed that LAP and HK did not show enzymatic activity and EST showed two alleles(est-sand and est-f while all the others were shown to be monomorphic. The allele EST-S showed a frequency of 82,6%.

  3. Değişik oranlarda kalsiyum karbonat kapsayan topraklarda çinkolu gübrelemenin mısır gelişimi ve antioksidatif enzim aktivitesine etkisi

    OpenAIRE

    Merken, Özen; Aydın, Mehmet

    2008-01-01

    Bu çalısmada farklı kireç seviyelerdeki çinko dozlarının mısır bitkisinde besin elementi içerikleri, bazı gelisme parametreleri ve antioksidatif enzim aktiviteleri üzerine etkileri arastırılmıstır. Kireç uygulaması ile bitki boyu, yaprak sayısı, kuru madde verimi, yaprak süperoksit dismutaz aktivitesi, klorofil ve protein çinko, kalsiyum ve magnezyum içerikleri artmıs, buna karsın fosfor, potasyum, demir, çinko ve bakır içerikleri düsmüstür. Çinko uygulaması ile ise bitki boyu, ya...

  4. Evaluating Ambient Displays in the Wild

    DEFF Research Database (Denmark)

    Messeter, Jörn; Molenaar, Daryn

    A prominent issue for evaluating ambient displays has been the conflict between the relative intrusiveness of evaluation methods and the intention to keep the display at the periphery of the user’s attention. There is a general lack of research discussing the difficulties of evaluating ambient di...

  5. Hybrid Logical Analyses of the Ambient Calculus

    DEFF Research Database (Denmark)

    Bolander, Thomas; Hansen, Rene Rydhof

    2010-01-01

    In this paper, hybrid logic is used to formulate three control flow analyses for Mobile Ambients, a process calculus designed for modelling mobility. We show that hybrid logic is very well-suited to express the semantic structure of the ambient calculus and how features of hybrid logic can...

  6. Ambient air contamination: Characterization and detection techniques

    Science.gov (United States)

    Nulton, C. P.; Silvus, H. S.

    1985-01-01

    Techniques to characterize and detect sources of ambient air contamination are described. Chemical techniques to identify indoor contaminants are outlined, they include gas chromatography, or colorimetric detection. Organics generated from indoor materials at ambient conditions and upon combustion are characterized. Piezoelectric quartz crystals are used as precision frequency determining elements in electronic oscillators.

  7. Embedded systems design issues in ambient intelligence

    NARCIS (Netherlands)

    Aarts, E.H.L.; Roovers, R.L.J.; Basten, A.A.; Geilen, M.C.W.; Groot, de H.W.H.

    2003-01-01

    The vision of ambient intelligence opens a world of unprecedented ex.periences: the interaction of people with electronic devices is changed as context awareness, natural interfaces and ubiquitous availability of information are realized. We analyze the consequences of the ambient intelligence

  8. Freedom and privacy in ambient intelligence

    NARCIS (Netherlands)

    Brey, Philip A.E.

    2006-01-01

    This paper analyzes ethical aspects of the new paradigm of Ambient Intelligence, which is a combination of Ubiquitous Computing and Intelligent User Interfaces (IUI’s). After an introduction to the approach, two key ethical dimensions will be analyzed: freedom and privacy. It is argued that Ambient

  9. Control Flow Analysis for BioAmbients

    DEFF Research Database (Denmark)

    Nielson, Flemming; Nielson, Hanne Riis; Priami, C.

    2007-01-01

    This paper presents a static analysis for investigating properties of biological systems specified in BioAmbients. We exploit the control flow analysis to decode the bindings of variables induced by communications and to build a relation of the ambients that can interact with each other. We...

  10. Spatial Analysis of BioAmbients

    DEFF Research Database (Denmark)

    Nielson, Hanne Riis; Nielson, Flemming; Pilegaard, Henrik

    2004-01-01

    Programming language technology can contribute to the development and understanding of Systems Biology by providing formal calculi for specifying and analysing the dynamic behaviour of biological systems. Our focus is on BioAmbients, a variation of the ambient calculi developed for modelling...

  11. Contabilidade ambiental o passaporte para a competitividade

    Directory of Open Access Journals (Sweden)

    Maria Elisabeth Pereira Kraemer

    2002-03-01

    Full Text Available Como todos os problemas ambientais provocaram grandes danos irreversíveis, que incidiram e incidem agora em todos os países subdesenvolvidos e desenvolvidos, nasce, então, como necessidade imperiosa, introduzir nos nossos sistemas econômicos a Contabilidade Ambiental, pois as organizações, até alguns anos atrás, preocupavam-se apenas com a eficiência dos sistemas produtivos. Chegamos a "era da revolução ambiental", e o ambiente não somente aparece como um conjunto de problemas relativos ao controle da contaminação, mas representa um custo no crescimento econômico. Neste sentido, o contador precisa de uma formação que se estenda além dos limites das técnicas e dos procedimentos. É necessário acrescentar o registro contábil do meio ambiente, dada a velocidade de sua afetação e a influência do mundo atual na utilização e disposição dos recursos naturais. Deve ser considerado como um dos princípios contábeis, onde é um desafio para a contabilidade. Precisa ser considerada como uma ciência contábil integrada, pois com a ciência ecológica e outras ciências são responsáveis pela natureza onde devem ser aplicados critérios de proteção ambiental, demonstrando que os recursos naturais constituem nosso principal capital. Partindo dessa premissa é que este trabalho está enfocando os seguintes tópicos: Responsabilidade Social da Empresa; A Empresa e o Meio Ambiente; Gestão Ambiental; Sistema de Gestão Ambiental; Ações para Preservar o Meio Ambiente - O Protocolo Verde, A Contabilidade, Custos Ambientais, Ativo Ambiental, Passivo Ambiental, Patrimônio Ambiental, Contabilidade Ambiental e Contabilidade Ambiental - o Passaporte para a Competitividade.

  12. CONFLITOS INTERPESSOAIS NO AMBIENTE ORGANIZACIONAL

    Directory of Open Access Journals (Sweden)

    Gabriela Paganini de Souza

    2014-07-01

    Full Text Available O presente artigo analisa e confirma a existência de impactos ocasionados pelos conflitos em um ambiente organizacional, através de pesquisa de campo realizada a partir de um estudo de caso e a comparação com a literatura adotada. A empresa escolhida foi abordada a partir de questionários, que foram respondidos pelos colaboradores e também pelo gestor. Percebeu-se que esses impactos são complexos de mensurar, principalmente os financeiros, que normalmente ficam ocultos por muito tempo. Entretanto, não se pode negar sua existência, pois estes tendem à piorar quando não são considerados.  Foi observado com a pesquisa que a conduta adotada para intermediar os conflitos está diretamente ligada ao sucesso de impedir impactos negativos aos indivíduos e à organização como um todo. Essas condutas, quando bem aplicadas podem contribuir reverter o impacto de um conflito, fazendo com que ele seja um fator motivador de mudanças e um estímulo para que os indivíduos demonstrem seus potenciais.

  13. GENES, POBLACIONES, AMBIENTES Y NACIONES

    Directory of Open Access Journals (Sweden)

    Noem\\u00ED Acreche

    2010-01-01

    Full Text Available Se estudió la estructura genética de 32 poblaciones de Argentina, Bolivia y Paraguay en función de las frecuencias génicas de ocho sistemas de grupos eritocitarios. Los sistemas incluidos son: ABO, MN, Ss, DI, P, Cc, Dd y Ee. En el caso de Bolivia, Paraguay y el Chaco Argentino, los datos fueron obtenidos de la bibliografía. La relación entre las poblaciones y diferentes criterios de agrupación (Ambiente, País, Grupo Lingüístico y Altitud fueron evaluadas por medio de Análisis Discriminante. Se consideró el porcentaje de casos correctamente clasificados como medida de la relación entre el conjunto de variables analizadas y el criterio de agrupación. Se encontró que tanto las categorías geoestructurales como lingüísticas establecidas tienen mayor relación con la estructura genética de las poblaciones incluidas en el análisis que las fronteras nacionales, por lo que se concluye que el aislamiento reproductivo entre poblaciones, necesario para la diferenciación, se produce por diferencias culturales o del territorio de ocupación antes que por las fronteras políticas establecidas.

  14. Calidad ambiental interior: bienestar, confort y salud

    Directory of Open Access Journals (Sweden)

    Francisco Vargas Marcos

    2005-01-01

    Full Text Available Distintas formas de interpretar las condiciones ambientales han llevado al desarrollo de conceptos tales como edificio enfermo, calidad del aire o calidad ambiental interior, todos ellos encaminados a entender la complejidad de los contaminantes en los ambientes cerrados y las implicaciones sobre la salud de la población. La propuesta de "Calidad ambiental interior" es un avance conceptual y operativo que supera ampliamente a los anteriores, puesto que orienta las acciones hacia ambientes saludables sin limitar al aire la idea de contaminación. El objetivo del trabajo es identificar las competencias y el marco legislativo que permiten actuar en la prevención de riesgos asociados a la exposición de contaminantes en ambientes interiores. Óptimas condiciones en los ambientes interiores deben redundar en salud, bienestar y confort, tanto en lo que respecta a la vida laboral como a los ámbitos donde se desarrollan las actividades cotidianas extralaborales, escolares, de descanso y de ocio. La sociedad actual exige lugares seguros, limpios y bien climatizados, para lo que es necesario integrar percepciones y exigencias de los habitantes y alcanzar un óptimo equilibrio entre estándares sociales, uso de la energía y desarrollo sostenible, buscando confort sin contaminar y sin aumentar el consumo de fuentes energéticas que degraden el medio ambiente. El desarrollo legislativo se orienta a la seguridad y la salud en los lugares de trabajo y la regulación de las sustancias químicas. La Sanidad Ambiental lleva a cabo tareas de prevención y control, participa en la ejecución de convenios internacionales de reducción de contaminantes y desechos y promueve acciones para el desarrollo de la Estrategia Europea de Salud y Medio Ambiente.

  15. Context Dependent Analysis of BioAmbients

    DEFF Research Database (Denmark)

    Pilegaard, Henrik; Nielson, Flemming; Nielson, Hanne Riis

    2006-01-01

    BioAmbients is a derivative of mobile ambients that has shown promise of describing interesting features of the behaviour of biological systems. The technical contribution of this paper is to extend the Flow Logic approach to static analysis with a couple of new techniques in order to give precise...... information about the behaviour of systems written in BioAmbients. Applying the development to a simple model of a cell releasing nutrients from food compunds we illustrate how the proposed analysis does indeed improve on previous efforts....

  16. Status of ambient air quality at Barauni

    International Nuclear Information System (INIS)

    Kannan, G.K.

    1993-01-01

    Due to industrialization, Barauni has become a well developed industrial estate to be considered as industrial hub of Bihar. Contemporary to the industrial growth, the environmental quality also gradually deteriorated. Hence a need was felt to know the status of ambient air quality for proper planning of the future growth of industries. The ambient air quality was monitored at 16 stations in and around Barauni industrial estate during 3 major seasons for the period of one year. The results are discussed as to the status of the ambient air quality and suggestion have also been made for improvement. (author). 5 refs., 2 figs., 7 tabs

  17. El dispositivo Problemática ambiental

    OpenAIRE

    Federico Di Pasquo; Tomas Busan; Gabriela Klier

    2018-01-01

    Se presenta y problematiza un dispositivo dirigido al tema de la problemática ambiental, del cual destaca su alcance global, su carácter de urgente y su capacidad para afectar a la viabilidad de la especie humana. A la vez, indicamos que esta caracterización de la crisis ambiental se encuentra asociada a una red institucional de corte internacional dirigida a la gestión de este tema. También, se señalan algunos intentos por utilizar a la problemática ambiental como coartada para el establecim...

  18. Ambient air pollution and thrombosis.

    Science.gov (United States)

    Robertson, Sarah; Miller, Mark R

    2018-01-03

    Air pollution is a growing public health concern of global significance. Acute and chronic exposure is known to impair cardiovascular function, exacerbate disease and increase cardiovascular mortality. Several plausible biological mechanisms have been proposed for these associations, however, at present, the pathways are incomplete. A seminal review by the American Heart Association (2010) concluded that the thrombotic effects of particulate air pollution likely contributed to their effects on cardiovascular mortality and morbidity. The aim of the current review is to appraise the newly accumulated scientific evidence (2009-2016) on contribution of haemostasis and thrombosis towards cardiovascular disease induced by exposure to both particulate and gaseous pollutants.Seventy four publications were reviewed in-depth. The weight of evidence suggests that acute exposure to fine particulate matter (PM 2.5 ) induces a shift in the haemostatic balance towards a pro-thrombotic/pro-coagulative state. Insufficient data was available to ascertain if a similar relationship exists for gaseous pollutants, and very few studies have addressed long-term exposure to ambient air pollution. Platelet activation, oxidative stress, interplay between interleukin-6 and tissue factor, all appear to be potentially important mechanisms in pollution-mediated thrombosis, together with an emerging role for circulating microvesicles and epigenetic changes.Overall, the recent literature supports, and arguably strengthens, the contention that air pollution contributes to cardiovascular morbidity by promoting haemostasis. The volume and diversity of the evidence highlights the complexity of the pathophysiologic mechanisms by which air pollution promotes thrombosis; multiple pathways are plausible and it is most likely they act in concert. Future research should address the role gaseous pollutants play in the cardiovascular effects of air pollution mixture and direct comparison of potentially

  19. Economía y medio ambiente

    Directory of Open Access Journals (Sweden)

    Ciro Alfonso Serna Mendoza

    2010-12-01

    Full Text Available En ciertos escenarios académicos, políticos, sociales y ambientales se declara que el modelo económico dominante o los conceptos que lo integran son responsables de que el bienestar económico implique malestar ecológico.Al partir de este supuesto, una forma de comprender las causas y de contribuir a la solución de la problemática ambiental es develar en qué consisten, en qué términos plantean la relación ombre-medio ambiente, las propuestas que incluyen la dimensión ambiental en el campo de la economía. Y si, al igual que las teorías ambientales, acuden a la ética como factor adecuado para disminuir las externalidades negativas generadas en el ambiente por la actividad económica.

  20. Games and Entertainment in Ambient Intelligence Environments

    NARCIS (Netherlands)

    Nijholt, Antinus; Reidsma, Dennis; Poppe, Ronald Walter; Aghajan, H.; López-Cózar Delgado, R.; Augusto, J.C.

    2009-01-01

    In future ambient intelligence (AmI) environments we assume intelligence embedded in the environment and its objects (floors, furniture, mobile robots). These environments support their human inhabitants in their activities and interactions by perceiving them through sensors (proximity sensors,

  1. 2011 NATA - Risks and Annual Ambient Concentrations

    Data.gov (United States)

    U.S. Environmental Protection Agency — This dataset includes the modeled annual ambient concentrations and risks at the census tract level for the 2011 National Air Toxics Assessment. All concentrations...

  2. A Smart Kitchen for Ambient Assisted Living

    OpenAIRE

    Blasco, Rubén; Marco, Álvaro; Casas, Roberto; Cirujano, Diego; Picking, Richard

    2014-01-01

    The kitchen environment is one of the scenarios in the home where users can benefit from Ambient Assisted Living (AAL) applications. Moreover, it is the place where old people suffer from most domestic injuries. This paper presents a novel design, implementation and assessment of a Smart Kitchen which provides Ambient Assisted Living services; a smart environment that increases elderly and disabled people’s autonomy in their kitchen-related activities through context and user awareness, appr...

  3. Unidades de Pediatría Ambiental

    OpenAIRE

    PARÍS M, ENRIQUE; MOLINA M, HELIA; RÍOS B, JUAN CARLOS

    2007-01-01

    Se considera que una Unidad de Pediatría Ambiental es una estructura con roles claramente definidos, situada preferentemente en un centro de salud, especializada en afecciones pediátricas, relacionadas al ambiente. Estos centros pueden proporcionar asesoramiento, información y tratamiento, promover la investigación, entrenar a profesionales, educar al público e informar a las autoridades responsables. Su personal, especialmente entrenado en problemas ambientales, incluye: pediatras, toxicólog...

  4. Ambient ionization mass spectrometry: A tutorial

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Min-Zong; Cheng, Sy-Chi; Cho, Yi-Tzu [Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan (China); Shiea, Jentaie, E-mail: jetea@fac.nsysu.edu.tw [Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan (China); Cancer Center, Kaohsiung Medical University, Kaohsiung, Taiwan (China)

    2011-09-19

    Highlights: {yields} Ambient ionization technique allows the direct analysis of sample surfaces with little or no sample pretreatment. {yields} We sort ambient ionization techniques into three main analytical strategies, direct ionization, direct desorption/ionization, and two-step ionization. {yields} The underlying principles of operation, ionization processes, detecting mass ranges, sensitivity, and representative applications of these techniques are described and compared. - Abstract: Ambient ionization is a set of mass spectrometric ionization techniques performed under ambient conditions that allows the direct analysis of sample surfaces with little or no sample pretreatment. Using combinations of different types of sample introduction systems and ionization methods, several novel techniques have been developed over the last few years with many applications (e.g., food safety screening; detection of pharmaceuticals and drug abuse; monitoring of environmental pollutants; detection of explosives for antiterrorism and forensics; characterization of biological compounds for proteomics and metabolomics; molecular imaging analysis; and monitoring chemical and biochemical reactions). Electrospray ionization and atmospheric pressure chemical ionization are the two main ionization principles most commonly used in ambient ionization mass spectrometry. This tutorial paper provides a review of the publications related to ambient ionization techniques. We describe and compare the underlying principles of operation, ionization processes, detecting mass ranges, sensitivity, and representative applications of these techniques.

  5. Ambient ionization mass spectrometry: A tutorial

    International Nuclear Information System (INIS)

    Huang, Min-Zong; Cheng, Sy-Chi; Cho, Yi-Tzu; Shiea, Jentaie

    2011-01-01

    Highlights: → Ambient ionization technique allows the direct analysis of sample surfaces with little or no sample pretreatment. → We sort ambient ionization techniques into three main analytical strategies, direct ionization, direct desorption/ionization, and two-step ionization. → The underlying principles of operation, ionization processes, detecting mass ranges, sensitivity, and representative applications of these techniques are described and compared. - Abstract: Ambient ionization is a set of mass spectrometric ionization techniques performed under ambient conditions that allows the direct analysis of sample surfaces with little or no sample pretreatment. Using combinations of different types of sample introduction systems and ionization methods, several novel techniques have been developed over the last few years with many applications (e.g., food safety screening; detection of pharmaceuticals and drug abuse; monitoring of environmental pollutants; detection of explosives for antiterrorism and forensics; characterization of biological compounds for proteomics and metabolomics; molecular imaging analysis; and monitoring chemical and biochemical reactions). Electrospray ionization and atmospheric pressure chemical ionization are the two main ionization principles most commonly used in ambient ionization mass spectrometry. This tutorial paper provides a review of the publications related to ambient ionization techniques. We describe and compare the underlying principles of operation, ionization processes, detecting mass ranges, sensitivity, and representative applications of these techniques.

  6. Biotecnologia Ambiental. Aplicacions biotecnològiques a la millora del medi ambient

    OpenAIRE

    Blanch i Gisbert, Anicet

    2010-01-01

    La biotecnología ambiental comprende el conjunto de actividades tecnológicas que facilitan la comprensión y la gestión de los sistemas biológicos en el medio ambiente, con el fin de proveer productos y servicios. Actualmente, la gestión del medio ambiente y de sus recursos naturales no se comprende si no se realiza de manera sostenible. Los avances científicos y tecnológicos le están permitiendo a la biotecnología ambiental, el desarrollo de nuevas herramientas y aplicaciones con los que resp...

  7. Towards New Ambient Light Systems: a Close Look at Existing Encodings of Ambient Light Systems

    Directory of Open Access Journals (Sweden)

    Andrii Matviienko

    2015-10-01

    Full Text Available Ambient systems provide information in the periphery of a user’s attention. Their aim is to present information as unobtrusively as possible to avoid interrupting primary tasks (e.g. writing or reading. In recent years, light has been used to create ambient systems to display information. Examples of ambient light systems range from simple notification systems such as displaying messages or calendar event reminders, to more complex systems such as focusing on conveying information regarding health activity tracking. However, for ambient light systems, there is a broad design space that lacks guidelines on when to make use of light displays and how to design them. In this paper we provide a systematic overview of existing ambient light systems over four identified information classes derived from 72 existing ambient light systems. The most prominent encoding parameters among the surveyed ambient light systems are color, brightness, and their combination. By analyzing existing ambient light systems, we provide a first step towards developing guidelines for designing future ambient light systems.

  8. Production and action of an Aspergillus phoenicis enzymatic pool using different carbon sources Produção e ação de um pool enzimático de Aspergillus phoenicis com fontes de carbono diferentes

    Directory of Open Access Journals (Sweden)

    Vivian Machado Benassi

    2012-09-01

    Full Text Available Aspergillus phoenicis is an interesting heat tolerant fungus that can synthesize enzymes with several applications in the food industry due to its great hydrolytic potential. In this work, the fungus produced high enzymatic levels when cultivated on inexpensive culture media consisting of flakes from different origins such as cassava flour, wheat fibre, crushed soybean, agro-industrial wastes, starch, glucose or maltose. Several enzymatic systems were produced from these carbon sources, but amylase was the most evident, followed by pectinase and xylanase. Traces of CMCases, avicelase, lipase, β-xylosidase, β-glucosidase and α-glucosidase activities were also detected. Amylases were produced on rye flakes, starch, oat flakes, corn flakes, cassava flour and wheat fibre. Significant amylolytic levels were produced in the culture medium with glucose or when this sugar was exhausted, suggesting an enzyme in the constitutive form. Cassava flour, rye, oats, barley and corn flakes were also used as substrates in the hydrolytic reactions, aiming to verify the liberation potential of reducing sugars. Corn flakes induced greater liberation of reducing sugars as compared to the others. Thin layer chromatography of the reaction end products showed that the hydrolysis of cassava flour liberated maltooligosaccharides, but cassava flour and corn, rye, oats and barley flakes were hydrolyzed to glucose. These results suggested the presence of glucoamylase and α-amylase as part of the enzymatic pool of A. phoencis.Aspergillus phoenicis é um fungo termotolerante interessante, uma vez que pode sintetizar enzimas com diversas aplicações em indústrias alimentícias em função de seu grande potencial de hidrólise. Neste trabalho, verificou-se que esse fungo produziu níveis enzimáticos elevados, quando o mesmo foi cultivado em meio de cultura de baixo custo, constituído de flocos de diferentes origens, como farinha de mandioca, fibra de trigo, soja

  9. Lípidos estructurados obtenidos por interesterificación química y enzimática a partir de aceite de pescado y grasa de palmiste

    Directory of Open Access Journals (Sweden)

    Gioielli, Luiz Antonio

    2003-06-01

    Full Text Available Through the structured lipids is possible to obtain fatty acids which are used as nourishings or therapeutics on specific diseases or on abnormal metabolic conditions. They are also synthesized to improve or alter the physic and/or chemical characteristics of the triacylglycerols. The objective of this study was to obtain structured lipids by chemical and enzymatic interesterification from palm kernel fat and fish oil. The samples were analyzed for the solid fat content at the temperatures from 10 to 30 ºC. A mathematical model of multiple regression of the quadratic type was applied. The solid fat content depended on the palm kernel fat and on the binary interactions between palm kernel fat and fish oil. The negative coefficients to the solid fat content showed an antagonic effect, which is characteristic of eutectic interactions between fats. The solid fat content after chemical and enzymatic interesterification was very similar.A través de los lípidos estructurados es posible obtener ácidos grasos para fines nutritivos y terapéuticos, usados en enfermedades específicas o en condiciones metabólicas anormales. Los lípidos estructurados, también pueden ser sintetizados para mejorar o alterar las caracteristicas físicas y/o químicas de los triacilgliceroles. El objetivo del trabajo fue obtener lípidos estructurados por interesterificación química y enzimática a partir de grasa de palmiste y aceite de pescado. Fueron estudiadas seis muestras, representadas por dos muestras individuales y cuatro mezclas binárias. Las muestras fueron analizadas en cuanto el contenido de grasa sólida en la faja de temperaturas de 10 a 30 ºC. Se aplicó un modelo de regresión múltiple del tipo cuadrático. Fueron obtenidos lípidos estructurados que presentaron un comportamiento físico plástico, aumentando sus características de aplicación. Los resultados obtenidos en la interesterificación química como en la enzimática demostraron que

  10. Actividades enzimáticas antioxidativas em Cistus Ladanifer L. provenientes de áreas não contaminadas em elementos vestigiais Antioxidant enzymes activity of Cistus Ladanifer L. from areas non contaminated in trace elements

    Directory of Open Access Journals (Sweden)

    Erika Santos

    2011-07-01

    Full Text Available Avaliou-se a actividade das enzimas antioxidativas (catalase, peroxidase e superóxido dismutase e as concentrações em As, Cu, Pb e Zn nas folhas de duas populações de Cistus ladanifer L. colhidas, na Primavera e no Verão, em solos não contaminados em elementos vestigiais (Caldeirão e Pomarão e com condições climáticas diferentes. Nos solos das duas áreas, as concentrações totais e disponíveis (extracção com DTPA em elementos vestigiais foram baixas. A distribuição dos elementos pelas folhas novas e maduras foi semelhante entre áreas e estações do ano. As actividades enzimáticas variaram consoante a população. As folhas colhidas nas duas estações do ano, excepto as folhas novas de Verão do Pomarão, apresentaram actividade enzimática na fracção solúvel e iónica. Comparando as estações do ano e áreas de amostragem, constatou-se que as actividades enzimáticas representam um mecanismo de tolerância a vários factores de stresse (radiação UV, temperatura do ar elevada no verão, défice hídrico no verão, baixa-média fertilidade do solo e conteúdo de elementos vestigiais no solo, o que confere uma elevada plasticidade à espécie.The aim of this study was to evaluate the antioxidant enzymes activity (catalase, peroxidase and superoxide dismutase and the concentrations of As, Cu, Pb and Zn in leaves from two populations of Cistus ladanifer growing on soils non-contaminated with trace elements (Caldeirco and Pomarco, but with different climatic conditions and in two different seasons (spring and summer. In both areas, total and available concentrations (DTPA extraction of trace elements in soils were low. The distribution of elements for young and mature leaves was similar between areas and seasons. The enzyme activities varied with the plant population. Leaves collected in the two seasons, except young leaves from Pomarco collected in summer, showed enzymatic activity in the soluble and ionic fractions

  11. Controle do escurecimento enzimático e da firmeza de polpa em pêssegos minimamente processados Control of enzymatic browning and flesh firmness in fresh-cut peaches

    Directory of Open Access Journals (Sweden)

    Amauri Costa da Costa

    2011-06-01

    Full Text Available O escurecimento enzimático e o amolecimento são as principais alterações que podem afetar pêssegos minimamente processados (MP. Objetivou-se avaliar a aplicação de L-cisteína (Cis, ácido L-ascórbico (AA e cloreto de cálcio (CC no controle do escurecimento enzimático e da perda de firmeza de polpa de pêssegos MP, cv. 'Esmeralda'. Os frutos foram processados e, após o fatiamento, imersos nos tratamentos: (I água (controle; (II AA+CC; (III Cis+CC; e, (IV Cis+AA+CC. Em seguida, as fatias foram acondicionadas em embalagens rígidas envoltas com filme PVC de 30µm de espessura e armazenadas durante seis dias a 4±1°C e 85±3% UR. As amostras foram avaliadas quanto aos aspectos físico-químicos, enzimáticos e sensoriais a cada dois dias. O tratamento Cis+AA+CC resultou em maiores valores de L* e h°, o que indica ter sido o mais eficaz na prevenção do escurecimento. Os tratamentos com L-cisteína resultaram em menor atividade da polifenoloxidase (PPO e o tratamento Cis+AA+CC foi o mais efetivo na contenção da atividade da peroxidase (POD, indicando que o melhor desempenho desse tratamento está associado à menor atividade de PPO e POD. A avaliação sensorial indicou que o tratamento Cis+AA+CC foi o mais eficiente, proporcionando a manutenção de características sensoriais aceitáveis aos pêssegos MP, cv. 'Esmeralda'.The enzymatic browning and firmness loss are the major changes that could affect fresh-cut peaches. The objective was to evaluate the application of L-cysteine (Cis, L-ascorbic acid (AA and calcium chloride (CC in the control of browning enzymatic and loss of firmness of minimally processed dried peaches, cv. 'Esmeralda'. The fruits were processed industrially and, after slicing, immersed in treatments: (I water (control; (II AA+CC; (III Cis+CC and (IV Cis+AA+CC. After, the slices were packed in packages sealed with PVC film 30µm thick and stored for six days at 4±1°C and 85±3% UR. The samples were evaluated

  12. Efecto de la aplicación de tratamientos combinados de aditivos sobre la inhibición del pardeamiento enzimático en manzanas cv. Granny Smith mínimamente procesadas

    Directory of Open Access Journals (Sweden)

    DENOYA, G. I.

    2012-10-01

    Full Text Available El pardeamiento enzimático, catalizado principalmente por la Enzima Polifenol Oxidasa (PPO, es uno de los principales problemas que afectan la calidad y limitan la vida útil de frutas y hortalizas mínimamente procesadas.Los compuestos tradicionalmente utilizados para inhibir la PPO, son los sulfitos. Sin embargo, se ha desalentado su utilización en la industria alimentaria debido a que se han registrado casos de reacciones alérgicas, especialmente en individuos asmáticos. Como consecuencia, en la actualidad, se evalúa la utilización de otros compuestos como potenciales inhibidores de la enzima, para garantizar productos frescos y naturales. En el presente trabajo, se analizó la evolución de la actividad de la enzima PPO y las características cromáticas de la pulpa de rodajas de manzanas cv. Granny Smith, tratadas por inmersión en una solución de aditivos. Los tratamientos utilizados fueron: I. 2% ácido ascórbico + 1% ácido cítrico + 0,5% EDTA, II. 1% ácido ascórbico + 0,5 % ácido cítrico + 0,25% EDTA y III. agua, empleada como control. Durante el almacenamiento, se evaluaron las coordenadas de color del espacio CIE L*a*b* de las rodajas y se demostró que los tratamientos I y II fueron efectivos en evitar el pardeamiento de la fruta. La evaluación espectrofotométrica de la actividad enzimática de la PPO, en extractos de manzanas sometidas a los distintos tratamientos, mostró que el más severo (I fue el que produjo el mayor grado de inhibición de la enzima, en todos los tiempos analizados. Se propone evaluar a futuro, la efectividad de estos inhibidores “in vitro”, a efectos de compararlos con los resultados obtenidos en rodajas de manzana.

  13. Valores de referencia basados en el contexto genético de la actividad enzimática de la colinesterasa en una población colombiana: un paso hacia el diagnóstico personalizado

    Directory of Open Access Journals (Sweden)

    Luz Helena Sánchez

    2015-08-01

    Full Text Available Introducción. La determinación de la actividad enzimática de la colinesterasa se utiliza comúnmente en la vigilancia biológica de la exposición a pesticidas organofosforados y carbamatos. Sin embargo, los factores genéticos afectan la actividad de la colinesterasa, por lo que la integración de la información sobre genotipos y fenotipos en las pruebas de laboratorio clínico, incrementaría la precisión de los valores de referencia. Objetivo. Establecer los valores de referencia basados en el contexto genético para la actividad enzimática de la acetilcolinesterasa (AChE y la butirilcolinesterasa (BChE, en una población colombiana. Materiales y métodos. Se incluyeron 397 adultos sanos. La actividad de la acetilcolinesterasa y la de la butirilcolinesterasa, se determinaron en muestras de sangre por potenciometría y espectrofotometría, respectivamente. Los genotipos de los ACHE rs17880573 y BCHE rs1803274 se obtuvieron mediante el ensayo TaqMan y los valores de referencia se estimaron con el programa MedCalc®. Resultados. La frecuencia alélica fue de 10,58 % para rs17880573 A y de 8,82 % para rs1803274 A. Las personas con los genotipos rs1803274 AA y AG, mostraron una reducción en el promedio de la actividad de la butirilcolinesterasa de 20,69 % y de 10,92 %, respectivamente, comparados con aquellas con el genotipo GG. No se encontraron diferencias significativas en la actividad de la acetilcolinesterasa con respecto a los alelos y genotipos del rs17880573. Los valores de referencia determinados para esta población fueron de 0,62-0,98 ΔpH/h para acetilcolinesterasa y de 4796,3-10321,1 U/L para butirilcolinesterasa, en las personas portadoras del alelo rs1803274 A, y de 5768,2–11180,4 U/L, en las portadoras del genotipo rs1803274 GG. Conclusión. Se recomienda el uso de estos valores de referencia basados en el contexto genético para las colinesterasas, en la práctica clínica diaria en esta población.

  14. Análisis estereoespecífico de los triacil-sn-gliceroles de las grasas naturales por métodos enzimáticos y cromatografía líquida de alta eficacia

    Directory of Open Access Journals (Sweden)

    Ruiz Gutiérrez, V.

    1995-12-01

    Full Text Available This paper is a review in which difficulties for the determination of the fatty acid stereospecific positions in the triacyl-sn-glycerol molecules of natural fats are discussed. For determining the stereospecific composition of the triacylglycerols, theoretical models for positional distribution of fatty acids in natural fats can be a useful estimation. Also, the principal methods for the stereospecific analysis by means of enzymatic analysis and high performance liquid chromatography are included. In both methods, the isolated sn-glyceridic fractions are later analysed by gas chromatography in order to know their fatty acid composition. In conclusion, combinations of several different analytical techniques such as enzymatic methods, high performance liquid chromatography, thin layer chromatography and gas chromatography can furnish valuable information to determine the stereospecific composition of the triacyl-sn-glycerols.

    Este artículo es una revisión bibliográfica en la que se discute la complejidad que presenta la determinación de la composición estereoespecífica de los triacil-sn-gliceroles de las grasas naturales. Los modelos teóricos de distribución de los ácidos grasos en las grasas naturales pueden ser una estimación útil para la determinación de la composición estereoespecífica de sus triacil-sn-gliceroles. Además, se recogen los principales métodos de análisis enzimático y cromatografía liquida de alta eficacia para el análisis estereoespecífico. En ambos métodos, se aislan distintas fracciones sn-glicerídicas que son posteriormente analizadas por cromatografía de gases para conocer su composición en ácidos grasos. Como conclusión, puede señalarse que la información más valiosa para determinar la composición estereoespecífica de los triacil-sn-gliceroles resulta de la combinación de diferentes técnicas de análisis como son los análisis enzim

  15. Active packaging use to inhibit enzymatic browning of apples/ Uso de embalagem ativa na inibição do escurecimento enzimático de maçãs

    Directory of Open Access Journals (Sweden)

    Giulliano Amaral Viana

    2008-08-01

    Full Text Available The enzymatic browning is the most limiting factor of fruits and vegetables shelf-life. The objective of this study was to evaluate the effect of an active packaging incorporated with anti-oxidant agents to inhibit apple’s enzymatic browning. Cellulosic films were incorporated with cysteine and sulphite and used to cover apples divided in halves. Browning inhibition was measured by polyphenoloxidase activity and colour analysis (CIE Lab colour system. Low concentration of sulphite (1% showed efficient browning inhibition and higher concentration of cysteine (15% was necessary to reach the same results. Treatments containing cysteine and sulphite resulted in brighter apples and less browning compared with control. The quantity of sulphite released to apples was lower than the limit allowed by legislation, decreasing, in this way, the levels of additives ingested by the consumer. In this study, the effectiveness of active packaging in providing product conservation was confirmed by the inhibition of browning in apples.O escurecimento enzimático é um dos fatores mais limitantes da vida de prateleira de frutas e vegetais. O objetivo desse estudo foi avaliar o efeito do uso de embalagem ativa incorporada com agentes antioxidantes na inibição do escurecimento enzimático de maçãs. Os filmes foram produzidos a base de polímero celulósico e incorporados com sulfito e cisteína para recobrimento de maçãs divididas ao meio. Foi avaliada a inibição do escurecimento através da atividade da polifenoloxidase e pela análise de cor (sistema CIE Lab. Baixas concentrações de sulfito (1% mostraram-se eficientes na inibição do escurecimento das maçãs e altas concentrações de cisteína (15% foram necessárias para a obtenção do mesmo resultado. Os tratamentos tanto com sulfito quanto com cisteína, comparados com os tratamentos controle, proporcionaram maior brilho às maçãs e menor escurecimento. O teor de sulfito liberado para a ma

  16. La protección internacional del medio ambiente

    OpenAIRE

    Bou Franch, Valentín

    2017-01-01

    PowerPoint del Tema 14 de la asignatura "Derecho Internacional Público". Curso 2017-18. Tema 14. La protección internacional del medio ambiente: 1.- El Derecho Internacional del Medio Ambiente: caracteres específicos. 2.- Principios fundamentales del Derecho Internacional del Medio Ambiente. 3.- Medio ambiente y Desarrollo: el Desarrollo Sostenible.

  17. Características de qualidade do suco polposo de cajá (Spondias lutea L. obtido por extração mecânico-enzimática Quality characteristics of pulpy caja fruit juice (Spondias lutea L. obtained by enzymatic-mechanical extration

    Directory of Open Access Journals (Sweden)

    Antônio de Pádua Valença DA SILVA

    1997-12-01

    Full Text Available Cajás (Spondias lutea L., oriundas do município de Maranguape (CE - Brasil, em estádio de plena maturação foram processadas em escala-piloto para obtenção de suco integral através do processo de extração mecânico-enzimática. O produto final foi analisado com relação as suas características físico-químicas bem quanto ao perfil sensorial após estocagem de 120 dias à temperatura ambiente (28°C. A polpa foi tratada com 120 ppm de enzimas pectinolíticas (Pectinex Ultra SP-L por 30 min, à temperatura de 25°C, o pH natural (2,98, seguido o processo mecânico de extração. Foi empregado como processo de preservação do suco integral o método "hot-fill" + aditivos. Segundo o ponto de vista estatístico as características físico-químicas permaneceram inalteradas ao longo de 120 dias, apresentando os seguintes valores médios: pH (2,78, sólidos solúveis (11,19 °Brix, acidez titulável total (1,46 g% de ácido cítrico, açúcares redutores (6,91 g% em glicose, taninos (77,36 mg% de ácido tânico, cor (0,125 em leitura de absorbância a 440 nm, pectina (0,12 g% em pectato de cálcio e viscosidade (10,8 cps. A análise sensorial classificou o suco polposo como de "boa" e "muito boa", sendo que as maiores médias couberam aos parâmetros aparência, cor e sabor.Caja fruit (Spondias lutea L. from Maranguape (Ceará-Brazil obtained at the full maturity stage were processed at a pilot-scale level with the purpose to obtain pulpy juice through enzymatic-mechanical process. The final product was analysed regarding physical-chemical characteristics as well as in relation to sensorial profile after 120 days of storage at room temperature (28°C. The pulp was treated by 120 ppm of pectinolytics enzymes (Pectinex Ultra SP-L during 30 minutes at 25°C, keeping natural pulp pH (2,98. The "hot-fill " + aditives were used as a preservation process for the pulpy juice. At a statistical point of view physical-chemical characteristics

  18. The Aesthetics of the Ambient Video Experience

    Directory of Open Access Journals (Sweden)

    Jim Bizzocchi

    2008-01-01

    Full Text Available Ambient Video is an emergent cultural phenomenon, with roots that go deeply into the history of experimental film and video art. Ambient Video, like Brian Eno's ambient music, is video that "must be as easy to ignore as notice" [9]. This minimalist description conceals the formidable aesthetic challenge that faces this new form. Ambient video art works will hang on the walls of our living rooms, corporate offices, and public spaces. They will play in the background of our lives, living video paintings framed by the new generation of elegant, high-resolution flat-panel display units. However, they cannot command attention like a film or television show. They will patiently play in the background of our lives, yet they must always be ready to justify our attention in any given moment. In this capacity, ambient video works need to be equally proficient at rewarding a fleeting glance, a more direct look, or a longer contemplative gaze. This paper connects a series of threads that collectively illuminate the aesthetics of this emergent form: its history as a popular culture phenomenon, its more substantive artistic roots in avant-garde cinema and video art, its relationship to new technologies, the analysis of the viewer's conditions of reception, and the work of current artists who practice within this form.

  19. Evaluación ambiental del PRMC

    Directory of Open Access Journals (Sweden)

    Waldo López Moya

    2001-12-01

    Full Text Available El presente artículo es una revisión constructiva de las actuales disposiciones sobre la evaluación ambiental de los planes reguladores, intentada con el propósito de contribuir a mejorar los procedimientos en práctica. En este sentido, aboga porque al plan regulador no se le exijan contribuciones ambientales que excedan a sus verdaderas competencias, para lo cual introduce el concepto de "efectos territoriales"; justifica que su evaluación ambiental deba ser realizda mediante una "declaración", y ahonda en la forma de elaborar y presentar esta declaración, acogiendo lo que a este respecto estipula el Art. 15° del Reglamento de la ley sobre Bases Generales del Medio Ambiente.

  20. Ambient Field Analysis at Groningen Gas Field

    Science.gov (United States)

    Spica, Z.; Nakata, N.; Beroza, G. C.

    2016-12-01

    We analyze continuous ambient-field data at Groningen gas field (Netherlands) through cross-correlation processing. The Groningen array is composed of 75 shallow boreholes with 6 km spacing, which contain a 3C surface accelerometer and four 5-Hz 3C borehole geophones spaced at 50 m depth intervals. We successfully retrieve coherent waves from ambient seismic field on the 9 components between stations. Results show high SNR signal in the frequency range of 0.125-1 Hz, and the ZZ, ZR, RZ, RR and TT components show much stronger wave energy than other components as expected. This poster discuss the different type of waves retrieved, the utility of the combination of borehole and surface observations, future development as well as the importance to compute the 9 components of the Green's tensor to better understand the wave field propriety with ambient noise.

  1. Ocean Ambient Noise Measurement and Theory

    CERN Document Server

    Carey, William M

    2011-01-01

    This book develops the theory of ocean ambient noise mechanisms and measurements, and also describes general noise characteristics and computational methods.  It concisely summarizes the vast ambient noise literature using theory combined with key representative results.  The air-sea boundary interaction zone is described in terms of non-dimensional variables requisite for future experiments.  Noise field coherency, rare directional measurements, and unique basin scale computations and methods are presented.  The use of satellite measurements in these basin scale models is demonstrated.  Finally, this book provides a series of appendices giving in-depth mathematical treatments.  With its complete and careful discussions of both theory and experimental results, this book will be of the greatest interest to graduate students and active researchers working in fields related to ambient noise in the ocean.

  2. Ambient air pollution and low birth weight

    DEFF Research Database (Denmark)

    Westergaard, Nadja; Gehring, Ulrike; Slama, Rémy

    2017-01-01

    (TLBW, restriction (IUGR), and suggest that some subgroups of pregnant women who are smoking, of low or high body-mass index (BMI), low socioeconomic status (SES) or asthma are more vulnerable towards...... on the association between ambient air pollution and TLBW. The adjusted odds ratio (OR) for TLBW associated with exposure to ambient air pollution were in one study higher among women who smoked during pregnancy, as compared to the OR of non-smoking women, while in the other study the association was in the opposite...... direction. The association of ambient air pollution and TLBW were higher among women characterized by extreme BMI (two studies) and low SES compared to non-obese women or women of higher SES (four studies), respectively. Only one study reported the estimated effects among asthmatic and non-asthmatic women...

  3. Ambient Air Pollution and Morbidity in Chinese.

    Science.gov (United States)

    Hu, Li-Wen; Lawrence, Wayne R; Liu, Yimin; Yang, Bo-Yi; Zeng, Xiao-Wen; Chen, Wen; Dong, Guang-Hui

    2017-01-01

    The rapid economic growth in China is coupled with a severe ambient air pollution, which poses a huge threat to human health and the sustainable development of social economy. The rapid urbanization and industrialization over the last three decades have placed China as one of countries with the greatest disease burden in world. Notably, the prevalence rate of chronic noncommunicable diseases (CND), including respiratory diseases, CVD, and stroke, in 2010 reaches 16.9%. The continuous growth of the incidence of CND urgent needs for effective regulatory action for health protection. This study aims to evaluate the impact of rapid urbanization on status of ambient air pollution and associated adverse health effects on the incidence and the burden of CND and risk assessment. Our findings would be greatly significant in the prediction of the risk of ambient air pollution on CND and for evidence-based policy making and risk management in China.

  4. Enzymatic technology to improve oil extraction from Caryocar brasiliense camb. (Pequi Pulp. Tecnologia enzimática para melhorar a extração do óleo da polpa de Caryocar brasiliense (pequi

    Directory of Open Access Journals (Sweden)

    Renata Gomes de Brito Mariano

    2009-09-01

    Full Text Available The present study aims to compare yield and quality of pequi pulp oil when applying two distinct processes: in the first, pulp drying in a tray dryer at 60ºC was combined with enzymatic treatment and pressing to oil extraction; in the second, a simple process was carried out by combining sun-drying pulp and pressing. In this study, raw pequi fruits were collected in Mato Grosso State, Brazil. The fruits were autoclaved at 121ºC and stored under refrigeration. An enzymatic extract with pectinase and CMCase activities was used for hydrolysis of pequi pulp, prior to oil extraction. The oil extractions were carried out by hydraulic pressing, with or without enzymatic incubation. The oil content in the pequi pulp (45% w/w and the physicochemical characteristic of the oil was determined according to standard analytical methods. Free fatty acids, peroxide values, iodine and saponification indices were respectively 1.46 mgKOH/g, 2.98 meq/kg, 49.13 and 189.40. The acidity and peroxide values were lower than the obtained values in commercial oil samples, respectively 2.48 mgKOH/g and 5.22 meq/kg. Aqueous extraction has presented lower efficiency and higher oxidation of unsaturated fatty acids. On the other hand, pequi pulp pressing at room temperature has produced better quality oil. However its efficiency is still smaller than the combined enzymatic treatment and pressing process. This combined process promotes cellular wall hydrolysis and pulp viscosity reduction, contributing to at least 20% of oil yield increase by pressing.O presente estudo tem como objetivo comparar o rendimento e a qualidade do óleo da polpa de pequi obtido por dois processos distintos: no primeiro, a secagem da polpa, conduzida em secador de bandeja a 60ºC, foi combinada com tratamento enzimático e prensagem para extração de óleo, no segundo, um processo mai simples foi realizado combinando-se a secagem da polpa ao sol seguida da prensagem a frio. Neste estudo, frutos de

  5. Hybrid Logical Analyses of the Ambient Calculus

    DEFF Research Database (Denmark)

    Bolander, Thomas; Hansen, René Rydhof

    2007-01-01

    In this paper, hybrid logic is used to formulate a rational reconstruction of a previously published control flow analysis for the mobile ambients calculus and we further show how a more precise flow-sensitive analysis, that takes the ordering of action sequences into account, can be formulated...... in a natural way. We show that hybrid logic is very well suited to express the semantic structure of the ambient calculus and how features of hybrid logic can be exploited to reduce the "administrative overhead" of the analysis specification and thus simplify it. Finally, we use HyLoTab, a fully automated...

  6. Comercio Internacional y Medio Ambiente en Colombia

    OpenAIRE

    Pérez Rincón, Mario Alejandro

    2006-01-01

    Consultable des del TDX A portada: Programa de Doctorado en Ciencias Ambientales, Opción Economía Ecológica y Gestión Ambiental A portada: Institut de Ciència i Tecnologia Ambientals (ICTA) Títol obtingut de la portada digitalitzada Desde la perspectiva de la Economía Ecológica, el Comercio Internacional no es un juego de suma positiva donde todos los participantes resultan ganadores por el intercambio comercial, sino que es un juego de suma cero, donde hay beneficiados y perjudic...

  7. Territorios vulnerables e injusticia ambiental en Argentina

    OpenAIRE

    Miguel Lacabana; Clara Bressano

    2016-01-01

    Justicia ambiental es un concepto fructífero en tanto modificó la perspectiva clásica de análisis sobre el problema de la degradación ambiental al establecer como principal interrogante no tanto las "causas" que producen tal degradación sino que interroga el proceso político, económico y social que consolida la desigual distribución social de las cargas nocivas de la contaminación. El conurbano sur de Buenos Aires y dentro de este el Municipio de Quilmes condensan distintas situaciones se vul...

  8. Human-Centric Interfaces for Ambient Intelligence

    CERN Document Server

    Aghajan, Hamid; Delgado, Ramon Lopez-Cozar

    2009-01-01

    To create truly effective human-centric ambient intelligence systems both engineering and computing methods are needed. This is the first book to bridge data processing and intelligent reasoning methods for the creation of human-centered ambient intelligence systems. Interdisciplinary in nature, the book covers topics such as multi-modal interfaces, human-computer interaction, smart environments and pervasive computing, addressing principles, paradigms, methods and applications. This book will be an ideal reference for university researchers, R&D engineers, computer engineers, and graduate s

  9. No queremos medio ambiente, lo queremos completo

    OpenAIRE

    Hederich G., Federico

    2018-01-01

    Sí bien es evidente la relación Hombre Medio Ambiente, no todos tenemos clara la manera como interactuamos interrumpiendo el desarrollo armónico de esta relación.El primer punto que vamos a tratar pretende esclarecer el significado de dos palabras básicas cuando se trata el tema del medio ambiente, en primera instancia, el término «basura» que se define como inmundicia, estiércol, y luego analizaremos «desperdicio» que significa derroche, residuo.Sí analizamos profundamente estas dos palabras...

  10. A RESPONSABILIDADE CIVIL E O MEIO AMBIENTE

    Directory of Open Access Journals (Sweden)

    DELCYLENE VILLALBA SOARES

    2012-06-01

    Full Text Available RESUMOEste artigo tem por finalidade realizar uma análise sobre a evolução da responsabilidade civil e suas teorias, subjetiva e objetiva aplicadas ao meio ambiente. Dentro desta área, deve-se ressaltar que a responsabilidade civil ao meio ambiente, é um assunto de importante relevância para o efetivo controle da depredação ambiental, permitindo que sejam alcançadas as metas propostas como condição de sobrevivência da espécie humana, ou seja, a conservação do meio ambiente e o desenvolvimento econômico social, visando desta maneira, permitir a qualidade e a continuação da vida. Existe o entendimento, que somente com educação ambiental e normas reguladoras fortes, como a penalidade de ter que indenizar quando praticar algum dano ao meio ambiente conseguirá atingir o objetivo maior de preservação. Necessitam-se além de uma mudança na legislação que responsabilize poluidores do meio ambiente, atribuindo-lhes sanções que inibam a prática de tal conduta ilícita e realizando assim, a aplicabilidade da justiça.Palavras-chave:Responsabilidade Civil; Responsabilidade; Meio Ambiente ABSTRACTThis article have objective to realize a analysis about civil responsibility evolution and your theories, subjective and objective apply environment. Inside this area have to stand out that civil responsibility environment, it is a theme very important relevance for environment depredation control effective, permitting have been obtain proposals target like been human survival condition, therefore, environment conservation and social economic development objective permit quality and continuation of life. There is understanding, that only with environment education and strong regulation principle, like penalty has to indemnify when practice some injury of environment, will obtain more preservation. Demand a change on legislation that responsible environment polluter attribute him inhibit sanctions for practice this illicit conduct and

  11. CUIDADO AMBIENTAL E RESPONSABILIDADE: POSSÍVEL DIÁLOGO ENTRE PSICOLOGIA AMBIENTAL E LOGOTERAPIA

    Directory of Open Access Journals (Sweden)

    Diogo Arnaldo Corrêa

    2016-05-01

    Full Text Available O cuidado ambiental vem sendo marcado pela tônica da conservação e da promoção da sustentabilidade. A premente necessidade de um dado compromisso e educação ambiental e de condutas pró-ambientais vem sendo defendida em vários contextos: na mídia, nas políticas públicas, por meio de campanhas sazonais. Todavia, pouco se descreve acerca do sentido presente nos modos de cuidado configurados a partir da inter-relação pessoa-ambiente. Nesta perspectiva, este estudo objetivou promover um possível diálogo a partir das contribuições da Psicologia Ambiental e da Logoterapia visando aproximar a noção de cuidado ambiental, delineada pela Psicologia Ambiental, à ideia de responsabilidade, como defendida pela Logoterapia, por meio de uma revisão bibliográfica. As articulações propositadas corroboraram, portanto, para acenar que a relação pessoa-ambiente pode ser arraigada em qualidade se a responsabilidade é reconhecida em sua propriedade de potencial humano, o que pode favorecer na emergência do sentido das atitudes e condutas pró-ambientais e explicitar o caráter de tarefa única e irrepetível inerente à vida.

  12. Nonsymbolic Gestural Interaction for Ambient Intelligence

    DEFF Research Database (Denmark)

    Rehm, Matthias

    2010-01-01

    the addressee with subtle clues about personality or cultural background. Gestures are an extremly rich source of communication-specific and contextual information for interactions in ambient intelligence environments. This chapter reviews the semantic layers of gestural interaction, focusing on the layer...

  13. EXPERIENCIA INTEGRADORA PARA EDUCACIÓN AMBIENTAL

    Directory of Open Access Journals (Sweden)

    Jorge F. Coronel

    2015-01-01

    Full Text Available En este trabajo se relacionó la práctica experimental de laboratorio co n el análisis de problemas ambientales. El objetivo de este trabajo es analizar una propuesta que vinculó el trabajo experimental con la situación del ambiente local para promover el cuidado de la naturaleza y su uso con sostenibilidad . Esta estrategia brindó un enfoque integrador aportando a los alumnos de profesorado una alternativa de aprendizaje con exper iencias sencillas para el estudio cualitativo de l ambiente local y motivar a l análisis de problemas asociad o s. Las experiencias permitieron caracterizar las muestras de suelo, agua y aire del lugar de residencia de los alumnos. La información experimental obtenida potenció la discusión de la situación ambiental actual. E l enfoque de enseñanza basado en problemas socio - científicos posibilit ó la visión interdisciplinar del tema , redimensionando valores y acciones para el cuidado ambiental y el desarrollo sustentable.

  14. Dynamical cognitive services for ambient intelligence environments

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.; Lee, C.-S.; Wang, M.-H.

    2009-01-01

    Ambient Intelligence systems are more than a simple integration among computer technologies, indeed, their design can strongly depend upon psychology and social sciences aspects describing, analyzing and forecasting the human being status during the system's decision making. Consequently, if from a

  15. Visualizing ambient user experiences: any how

    NARCIS (Netherlands)

    Kanis, M.; Robben, S.; Veenstra, M.; Kröse, B.; Olivier, P.; Wright, P.

    2012-01-01

    As interactive systems become increasingly complex and entwined with the environment, technology is becoming more and more invisible. This means that much of the technology that people come across every day goes unnoticed and that the (potential) workings of ambient systems are not always clearly

  16. Revealing source signatures in ambient BTEX concentrations

    International Nuclear Information System (INIS)

    Zalel, Amir; Yuval; Broday, David M.

    2008-01-01

    Management of ambient concentrations of Volatile Organic Compounds (VOCs) is essential for maintaining low ozone levels in urban areas where its formation is under a VOC-limited regime. The significant decrease in traffic-induced VOC emissions in many developed countries resulted in relatively comparable shares of traffic and non-traffic VOC emissions in urban airsheds. A key step for urban air quality management is allocating ambient VOC concentrations to their pertinent sources. This study presents an approach that can aid in identifying sources that contribute to observed BTEX concentrations in areas characterized by low BTEX concentrations, where traditional source apportionment techniques are not useful. Analysis of seasonal and diurnal variations of ambient BTEX concentrations from two monitoring stations located in distinct areas reveal the possibility to identify source categories. Specifically, the varying oxidation rates of airborne BTEX compounds are used to allocate contributions of traffic emissions and evaporative sources to observed BTEX concentrations. - BTEX sources are identified from temporal variations of ambient concentration

  17. Ambient environment analysis by means of perception

    NARCIS (Netherlands)

    Bitterman, M.S.; Ciftcioglu, O.; Bhatt, M.; Schultz, C.

    2013-01-01

    Analysis of an ambient environment by means of perception is described. The surveillance of an object by human, who watches a scene via a monitor that shows camera sensed information, is investigated. Although the camera sensing process is a deterministic process, human perception of a scene via

  18. Ambient Surveillance by Probabilistic-Possibilistic Perception

    NARCIS (Netherlands)

    Bittermann, M.S.; Ciftcioglu, O.

    2013-01-01

    A method for quantifying ambient surveillance is presented, which is based on probabilistic-possibilistic perception. The human surveillance of a scene through observing camera sensed images on a monitor is modeled in three steps. First immersion of the observer is simulated by modeling perception

  19. Visualizing ambient user experiences : any how

    NARCIS (Netherlands)

    Kanis, Marije; Robben, Saskia; Kröse, Ben; Veenstra, Mettina

    2012-01-01

    As interactive systems become increasingly complex and entwined with the environment, technology is becoming more and more invisible. This means that much of the technology that people come across every day goes unnoticed and that the (potential) workings of ambient systems are not always clearly

  20. EXPOSURE TO CONCENTRATED AMBIENT PARTICLES (CAPS): REVIEW

    Science.gov (United States)

    Epidemiologic studies support a participation of fine particulate matter (PM) with a diameter of 0.1 to 2.5 microm in the effects of air pollution particles on human health. The ambient fine particle concentrator is a recently developed technology that can enrich the mass of ambi...

  1. Territorios vulnerables e injusticia ambiental en Argentina

    Directory of Open Access Journals (Sweden)

    Miguel Lacabana

    2016-07-01

    Full Text Available Justicia ambiental es un concepto fructífero en tanto modificó la perspectiva clásica de análisis sobre el problema de la degradación ambiental al establecer como principal interrogante no tanto las "causas" que producen tal degradación sino que interroga el proceso político, económico y social que consolida la desigual distribución social de las cargas nocivas de la contaminación. El conurbano sur de Buenos Aires y dentro de este el Municipio de Quilmes condensan distintas situaciones se vulnerabilidad social ambiental donde los conflictos son acallados, ocultos o simplemente quedan latentes frente a la emergencia de mantener o crear nuevas fuentes de empleo. La cuestión ambiental no se constituyó durante años en un problema con relevancia pública si bien existieron tímidas políticas públicas que tendieron a regular los efectos de polución industrial y sus efectos sobre la calidad de vida de las personas.

  2. Setting priorities for ambient air quality objectives

    International Nuclear Information System (INIS)

    2004-10-01

    Alberta has ambient air quality objectives in place for several pollutants, toxic substances and other air quality parameters. A process is in place to determine if additional air quality objectives are required or if existing objectives should be changed. In order to identify the highest priority substances that may require an ambient air quality objective to protect ecosystems and public health, a rigorous, transparent and cost effective priority setting methodology is required. This study reviewed, analyzed and assessed successful priority setting techniques used by other jurisdictions. It proposed an approach for setting ambient air quality objective priorities that integrates the concerns of stakeholders with Alberta Environment requirements. A literature and expert review were used to examine existing priority-setting techniques used by other jurisdictions. An analysis process was developed to identify the strengths and weaknesses of various techniques and their ability to take into account the complete pathway between chemical emissions and damage to human health or the environment. The key strengths and weaknesses of each technique were identified. Based on the analysis, the most promising technique was the tool for the reduction and assessment of chemical and other environmental impacts (TRACI). Several considerations for using TRACI to help set priorities for ambient air quality objectives were also presented. 26 refs, 8 tabs., 4 appendices

  3. Training Engineers for the Ambient Intelligence Challenge

    Science.gov (United States)

    Corno, Fulvio; De Russis, Luigi

    2017-01-01

    The increasing complexity of the new breed of distributed intelligent systems, such as the Internet of Things, which require a diversity of languages and protocols, can only be tamed with design and programming best practices. Interest is also growing for including the human factor, as advocated by the "ambient intelligence" (AmI)…

  4. RADIONUCLIDE TRANSPORT MODELS UNDER AMBIENT CONDITIONS

    Energy Technology Data Exchange (ETDEWEB)

    S. Magnuson

    2004-11-01

    The purpose of this model report is to document the unsaturated zone (UZ) radionuclide transport model, which evaluates, by means of three-dimensional numerical models, the transport of radioactive solutes and colloids in the UZ, under ambient conditions, from the repository horizon to the water table at Yucca Mountain, Nevada.

  5. Preparation of heterogeneous networks for ambient intelligence

    NARCIS (Netherlands)

    Stok, van der P.D.V.; Basten, A.A.; Geilen, M.C.W.; Groot, de H.W.H.

    2003-01-01

    Ambient Intelligence assumes the existence of ubiquitous networked computing. Networking is supported in the home by a so-called home network. Currently, the home network emerges from the purchase of a second PC in the home. Extending the home network confronts the prospective buyer with a multitude

  6. Hybrid Logical Analyses of the Ambient Calculus

    DEFF Research Database (Denmark)

    Bolander, Thomas; Hansen, René Rydhof

    2007-01-01

    In this paper, hybrid logic is used to formulate a rational reconstruction of a previously published control flow analysis for the mobile ambients calculus and we further show how a more precise flow-sensitive analysis, that takes the ordering of action sequences into account, can be formulated...

  7. A Conceptual Framework for Ambient Learning Displays

    NARCIS (Netherlands)

    Börner, Dirk; Kalz, Marco; Specht, Marcus

    2011-01-01

    Börner, D., Kalz, M., & Specht, M. (2010, 29 November-3 December). A Conceptual Framework for Ambient Learning Displays. Poster presented at the Work-in-Progress Poster and Invited Young Researcher Symposium of the 18th International Conference on Computers in Education, Putrajaya, Malaysia:

  8. A Conceptual Framework for Ambient Learning Displays

    NARCIS (Netherlands)

    Börner, Dirk; Kalz, Marco; Specht, Marcus

    2010-01-01

    Börner, D., Kalz, M., & Specht, M. (2010). A Conceptual Framework for Ambient Learning Displays. In B. Chang, T. Hirashima, & H. Ogata (Eds.), Joint Proceedings of the Work-in-Progress Poster and Invited Young Researcher Symposium for the 18th International Conference on Computers in Education (pp.

  9. A Kleene analysis of Mobile Ambients

    DEFF Research Database (Denmark)

    Nielson, Flemming; Nielson, Hanne Riis; Sagiv, M.

    2000-01-01

    We show how a program analysis technique originally developed for C-like pointer structures can be adapted to analyse the hierarchical structure of processes in the ambient calculus. The technique is based on modeling the semantics of the language in a two-valued logic; by reinterpreting...

  10. Dynamic services for open ambient intelligence systems

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.; Ma, Z.

    2006-01-01

    Ambient intelligence (AmI) [1] provides a wide-ranging vision on how the Information Society will evolve, since the goal is to conceive platforms for seamless delivery of services and applications making them effectively invisible to the user. This is possible by gathering best practices from

  11. Redox Toxicology of Ambient Air Pollution

    Science.gov (United States)

    Ambient air pollution is a leading global cause of morbidity and mortality. Millions of Americans live in areas in which levels of tropospheric ozone exceed air quality standards, while exposure to particulate matter (PM2.5) alone results in 3.2 million excess deaths annually wor...

  12. Radionuclide Transport Models Under Ambient Conditions

    International Nuclear Information System (INIS)

    Moridis, G.; Hu, Q.

    2001-01-01

    The purpose of Revision 00 of this Analysis/Model Report (AMR) is to evaluate (by means of 2-D semianalytical and 3-D numerical models) the transport of radioactive solutes and colloids in the unsaturated zone (UZ) under ambient conditions from the potential repository horizon to the water table at Yucca Mountain (YM), Nevada

  13. Automated Processing Workflow for Ambient Seismic Recordings

    Science.gov (United States)

    Girard, A. J.; Shragge, J.

    2017-12-01

    Structural imaging using body-wave energy present in ambient seismic data remains a challenging task, largely because these wave modes are commonly much weaker than surface wave energy. In a number of situations body-wave energy has been extracted successfully; however, (nearly) all successful body-wave extraction and imaging approaches have focused on cross-correlation processing. While this is useful for interferometric purposes, it can also lead to the inclusion of unwanted noise events that dominate the resulting stack, leaving body-wave energy overpowered by the coherent noise. Conversely, wave-equation imaging can be applied directly on non-correlated ambient data that has been preprocessed to mitigate unwanted energy (i.e., surface waves, burst-like and electromechanical noise) to enhance body-wave arrivals. Following this approach, though, requires a significant preprocessing effort on often Terabytes of ambient seismic data, which is expensive and requires automation to be a feasible approach. In this work we outline an automated processing workflow designed to optimize body wave energy from an ambient seismic data set acquired on a large-N array at a mine site near Lalor Lake, Manitoba, Canada. We show that processing ambient seismic data in the recording domain, rather than the cross-correlation domain, allows us to mitigate energy that is inappropriate for body-wave imaging. We first develop a method for window selection that automatically identifies and removes data contaminated by coherent high-energy bursts. We then apply time- and frequency-domain debursting techniques to mitigate the effects of remaining strong amplitude and/or monochromatic energy without severely degrading the overall waveforms. After each processing step we implement a QC check to investigate improvements in the convergence rates - and the emergence of reflection events - in the cross-correlation plus stack waveforms over hour-long windows. Overall, the QC analyses suggest that

  14. Sistemas de gestión ambiental normalizados

    OpenAIRE

    Fronti de García, Luisa; Pahlen, Ricardo José María; D’Onofrio, Paula Alejandra

    2004-01-01

    La contabilidad ambiental presupone la existencia de un sistema de gestión ambiental. Dentro del sistema integral de la organización, el sistema de gestión ambiental permite la obtención de información oportuna y segura. Podríamos definir el sistema de gestión ambiental de un ente como el conjunto de medidas destinadas a prevenir el impacto negativo de las actividades del ente sobre el medio ambiente. El sistema de gestión ambiental (SGA) es aquella parte del sistema de gestión total que incl...

  15. Significance and influence of the ambient temperature as a rate ...

    Indian Academy of Sciences (India)

    Unknown

    , undoubtedly is dependent even on the level of the ambient temperature. Therefore, the ambient temperature seems to be an important factor of the corrosion rate and the durability of the reinforced concrete structures in aggressive ...

  16. Utilização de complexo enzimático em dietas à base de sorgo-soja para frangos de corte Effects of feeding sorghum-soya of enzymatic supplementation on broiler chickens based diet

    Directory of Open Access Journals (Sweden)

    Maria do Socorro Vieira dos Santos

    2006-06-01

    Full Text Available Este experimento foi conduzido com os objetivos de avaliar o desempenho e o peso dos órgãos do trato gastrointestinal e analisar economicamente a produção de frangos de corte submetidos a dietas à base de sorgo e soja, suplementadas ou não com complexo multienzimático (xilanase, amilase, protease, nas fases inicial (experimento I e final (experimento II de crescimento. O delineamento estatístico foi em blocos ao acaso, em esquema fatorial 3 x 2 (três níveis de sorgo = 0, 50 e 100% e dois níveis de enzima = 0 e 0,1%, em que a unidade experimental foi constituída de um boxe com 17 aves e oito repetições por tratamento. No experimento I (1 a 21 dias, não houve efeito significativo do nível de substituição do milho pelo sorgo sobre o consumo de ração, o ganho de peso e a conversão alimentar. Entretanto, o acréscimo no nível de sorgo ocasionou redução no peso relativo da moela e a suplementação enzimática aumentou o peso relativo do jejuno e íleo. No experimento II (22 a 42 dias, não houve efeito significativo do nível de substituição do milho pelo sorgo sobre o consumo de ração, o ganho de peso, a conversão alimentar e os pesos relativos do duodeno, jejuno, íleo e ceco. A suplementação enzimática reduziu significativamente o rendimento de carcaça e aumentou o peso do proventrículo, da moela e do pâncreas. A análise econômica revelou que o sorgo pode substituir 50% do milho em rações para frangos de corte nas fases inicial e final do crescimento.The trial was conducted to evaluate the performance and morphologic changes of gastrointestinal tract and economic analysis for broilers fed sorghum-soybean meal-based diets, supplemented or not with multienzymatic complex (xilanase, amylase, protease, in the starting (experiment I and finishing (experiment II phases. The experiment was analyzed as a randomized block design with a 3 x 2 factorial arrangement [three sorghum levels (0, 50, and 100% x enzyme level

  17. Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus Biodegradação do azul brilhante de remazol R pelo complexo enzimático ligninolítico produzido por Pleurotus ostreatus

    Directory of Open Access Journals (Sweden)

    Kátia Maria Gomes Machado

    2006-12-01

    ,6. Descoloração do RBBR foi acompanhada a 592 nm e atividades de peroxidases e lacase pela oxidação do ABTS. Peroxidase da raiz forte (HRP foi usada como referência. O tempo de crescimento de P. ostreatus influenciou a degradação do RBBR e a produção das atividades enzimáticas de peroxidases e de lacase. Concentração de 1 mM de H2O2 e pH 4,0 mostraram-se ótimos para descoloração do RBBR. Descoloração total do RBBR foi obtida com adição de apenas uma alíquota de 50 µL de H2O2 (1 mM. Maior estabilidade dos extratos foi obtida por refrigeração que por congelamento. Foi evidenciado o potencial de aplicação de extratos enzimáticos de Pleurotus ostreatus e do resíduo da produção do cogumelo para a degradação de compostos xenobióticos.

  18. Efecto in vitro del D-002 sobre la actividad enzimática de la 5-lipoxigenasa (5-LOX Effect of D-002 on 5-lipooxygenase (5-LOX enzyme activity in vitro

    Directory of Open Access Journals (Sweden)

    Yohani Pérez Guerra

    2012-06-01

    Full Text Available Introducción: el D-002, mezcla de seis alcoholes alifáticos primarios de alto peso molecular purificada de la cera de abejas, ha mostrado efectos antiinflamatorios, sin gastrotoxicidad secundaria, enmodelos experimentales. El tratamiento oral con D-002 reduce las concentraciones de leucotrieno B4 (LTB4 en exudados pleurales de ratas con pleuresía por carragenina, sugiriendo que podría inhibir la actividad enzimática de la 5-lipooxigenasa (5-LOX, si bien los mecanismos de la acción antiinflamatoria del D-002 no habían sido aún explorados. Objetivo: evaluar el efecto in vitro del D-002 sobre la actividad de la 5-LOX, utilizando la fracción citosólica de homogenatos de hígado de ratas. Métodos: las condiciones de ensayo utilizadas fueron las siguientes: fracción citosólica (50 µg de proteína disuelta en solución reguladora borato 0,2 mol/L (pH 9 y ácido linoleico (7,8-250 mmol/L como sustrato, ensayándose muestras paralelas incubadas con Tween-20/H2O (2 % (vehículo, muestras controles, D-002 (0,9-1 000 µg/mL o Lyprinol (500 µg/mL (sustancia de referencia. La actividad enzimática se evaluó mediante el cambio de absorbancia a 234 nm producido por la formación de dienos conjugados y medido en espectrofotómetro UV-visible. Resultados: la adición in vitro del D-002 produjo una inhibición significativa, dependiente de la dosis (r= 0,980; pIntroduction: D-002, a mixture of six high molecular weight primary aliphatic alcohols purified from beeswax, has been shown to produce anti-inflammatory effects with no secondary gastrotoxicity in experimental models. Oral treatment with D-002 was effective for lowering the concentrations of B4 leukotriene (LTB4 in pleural exudates of rats with carragenin-induced pleurisy, suggesting that it could inhibit 5-lipooxigenase (5-LOX enzyme activity. The mechanisms of the anti-inflammatory action of D-002, however, had not been explored yet. Objective: to evaluate the effects of D-002 on 5-LOX

  19. The relationship between body and ambient temperature and corneal temperature

    DEFF Research Database (Denmark)

    Kessel, Line; Johnson, Leif; Arvidsson, Henrik Sven

    2010-01-01

    Exposure to elevated ambient temperatures has been mentioned as a risk factor for common eye diseases, primarily presbyopia and cataract. The aim of the present study was to examine the relationship among ambient, cornea, and body core temperature.......Exposure to elevated ambient temperatures has been mentioned as a risk factor for common eye diseases, primarily presbyopia and cataract. The aim of the present study was to examine the relationship among ambient, cornea, and body core temperature....

  20. Otimização da extração enzimática da proteína do feijão - doi: 10.4025/actascitechnol.v32i3.6277

    Directory of Open Access Journals (Sweden)

    Carlos de Oliveira Lopes Junior

    2010-11-01

    Full Text Available Considerando-se a importância do feijão na dieta do brasileiro e, ainda, que este alimento não é permitido na alimentação de fenilcetonúricos, é fundamental promover a extração de suas proteínas, visando à posterior remoção de fenilalanina. No intuito de se otimizar a extração proteica do feijão, dois métodos enzimáticos foram, inicialmente, empregados. Para aquele que produziu o maior rendimento de extração proteica (REP, foi realizado um estudo do efeito de diversos parâmetros, tais como velocidade de centrifugação, tempo de reação, pH inicial e tipo de enzima (uma de Bacillus licheniformis, duas de Bacillus subtilis, uma de Aspergillus sojae e uma de Papaya carica. Os resultados indicaram que o melhor método foi o que empregou uma concentração de matéria-prima de 1:10 (p v-1, pH inicial de 10,5, temperatura de 50°C, e ausência de agitação em ultraturrax. Com relação aos parâmetros testados, o maior REP (93,1% foi obtido ao se utilizar a enzima de Bacillus licheniformis, na velocidade de centrifugação de 10.640 x g, tempo de reação de 3h e pH inicial de 10,5.

  1. Enzymatic preparation and characterization of soybean lecithin-based emulsifiers; Preparación enzimática y caracterización de emulsionantes a base de lecitina de soja

    Energy Technology Data Exchange (ETDEWEB)

    Reddy Jala, R.C.; Chen, B.; Li, H.; Zhang, Y.; Cheong, L.Z.; Yang, T.; Xu, X.

    2016-07-01

    Simple enzymatic methods were developed for the synthesis of lysolecithin, glycerolyzed lecithin and hydrolyzed lecithin. The products were characterized in terms of their acetone insoluble matter, hexane insoluble matter, moisture, phospholipid distribution and fatty acid composition. The HLB value ranges of different products with different acid values were detected. The efficiency of optimally hydrolyzed lecithin was examined at high calcium ion, low pH, and aqueous solutions and compared with commercially available standard lecithin-based emulsifiers. Overall, lysolecithin powder was proven to be the best emulsifier even at strong and medium acidic conditions. [Spanish] Se han desarrollado métodos enzimáticos simples para la síntesis de lisolecitina, lecitina esterificada a glicerol y lecitina hidrolizada. Los productos se caracterizaron en términos de su composición en materia insoluble en acetona, materia insoluble en hexano, humedad, distribución de fosfolípidos y ácidos grasos. Además, se detectaron rangos de los valores de HLB de diferentes productos con valores de ácido diferentes. La eficiencia de la lecitina hidrolizada de forma óptima fue estudiada en función de una alta concentración de ion calcio, pH bajo, y soluciones acuosas y se compara con emulsionantes basados en lecitina estándar disponibles en el mercado. En general, el polvo de lisolecitina mostró ser el mejor emulsionante incluso en condiciones ácidas fuertes y medias.

  2. Efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA sobre a qualidade do suco de banana Effect of enzymatic clarifier complexes Clarex and CEC1-CTAA on the quality of banana juice

    Directory of Open Access Journals (Sweden)

    Marisa Helena Cardoso

    1999-05-01

    Full Text Available Neste trabalho foi observado o efeito dos complexos enzimáticos clarificantes Clarex e CEC1-CTAA, adicionados na proporção de 0,03% v/p sobre purê de banana (Musa cavendishii, em condições amenas de hidrólise (40ºC, 15 minutos visando determinar a qualidade, aqui representada pelos indicadores: rendimento; viscosidade; Brix; pH; composição centesimal; contagens de bolores e leveduras e de mesófilos, e propriedades sensoriais de cor, aroma, sabor e corpo dos sucos de banana clarificados. O suco clarificado com Clarex apresentou-se significativamente (p The effect of the clarifier enzymatic complexes Clarex and CEC1-CTAA, used in the proportion 0.03% v/w in industrialized banana (Musa cavendishii pulp, at the conditions of gentle hydrolysis 40 degree Celsius, 15 minutes, was observed to determine the quality here represented by indicators such as yield, viscosity, Brix, pH, centesimal composition, counts of moulds and yeasts and of mesophilics, and sensorial properties of color, aroma, flavor and body by both clarified banana juices. The juice clarified by Clarex was significantly (p < 0.01 more yellow, less grey, less opaque and less viscous than that obtained with CEC1-CTAA. There was no significant difference between the means of aroma of fresh banana and flavor in these juices. Furthermore, the values obtained for flavor for both juices were judged good (6.72 and 6.05 for the juices clarified with Clarex and CEC1- CTAA, respectively, because they were up the middle of the scale (from 0 to 10.

  3. Saúde Ambiental: uma Análise dos Resultados das Conferências Nacionais de Meio Ambiente, Saúde e Saúde Ambiental

    Directory of Open Access Journals (Sweden)

    Maria Beatriz Maury de Carvalho

    2010-08-01

    Full Text Available A representação nas políticas públicas do conceito de saúde ambiental, como expressão do equilíbrio da rede de conexões entre a sociedade humana e a natureza é discutida neste trabalho, no intuito de demonstrar o status quo da saúde ambiental nas políticas públicas no país. Primeiro foi traçado o histórico da “ambientalização na saúde”, do conceito de saúde ambiental e da institucionalização das políticas ambientais e de saúde ambiental no âmbito dos Ministérios do Meio Ambiente e da Saúde. As interações entre as políticas de Saúde e de Meio Ambiente para a efetivação de uma Política Nacional de Saúde Ambiental e as intersecções dos resultados das Conferências Nacionais de Saúde, Meio Ambiente e Saúde Ambiental foram analisadas; concluindo- se com os avanços conseguidos pelos dois setores, os desafios para a articulação intersetorial e a participação social para a construção de políticas públicas de saúde ambiental.

  4. MODIFICANDO O AMBIENTE ESCOLAR ATRAVÉS DO PROJETO MEIO AMBIENTE: CULTIVANDO E CONHECENDO AS PLANTAS

    Directory of Open Access Journals (Sweden)

    Letícia Caroline Lopes

    2011-07-01

    Full Text Available O presente trabalho intitulado “MODIFICANDO O AMBIENTE ESCOLAR ATRAVÉS DO PROJETO MEIO AMBIENTE: CULTIVANDO E CONHECENDO AS PLANTAS” tem como desígnio apresentar o desenvolvimento do projeto “meio ambiente: cultivando e conhecendo as plantas”, no qual teve como proposta sensibilizar os alunos sobre a importância e preservação do meio ambiente através da prática e modificação do ambiente escolar, onde procurou-se utilizar materiais reciclados, tais como, garrafas pet, pneus velhos, carreteis de fios de eletricidade, e também mudas de arvore, plantas e hortaliças. Desta forma buscou se utilizar da criatividade dos envolvidos no processo para a transformação do ambiente escolar seco e vazio, para uma ambiente alegre, e acolhedor. O projeto foi elaborado pelas professoras da Extensão Betel, e aplicado em parceria com acadêmicos do sexto semestre do curso de Licenciatura em Pedagogia da Universidade do Estado do Mato Grosso (UNEMAT, campus de Sinop. Tendo como proposta a interdisciplinaridade, o projeto foi divido em três eixos orientados pelas professoras Edneuza Alves Trugillo, Sinóvia Rauber e Mariza Kreinner, abrangendo assim alunos e professoras de duas turmas de 2º Ano do Ensino Fundamental, e acadêmicos e professores da Universidade, os eixos foram arborização, jardinagem e horta, onde teve a duração de aproximadamente dois meses. A finalização das práticas obteve resultados satisfatórios e objetivos alcançados, tanto pela sensibilização dos alunos sobre a preservação e importância do meio-ambiente, quanto pela prática de intervenção pelos acadêmicos envolvidos no processo, que em coletividade conseguiram modificar o ambiente escolar através do objetivo do projeto, meio-ambiente, proporcionando assim um enriquecimento na formação acadêmica enquanto práticas pedagógicas.

  5. Níveis decrescentes de proteína em dietas suplementadas com complexo enzimático, para coelhos em crescimento. 2. Parâmetros digestivos e composição química do conteúdo cecal

    Directory of Open Access Journals (Sweden)

    Santiago G.S.

    2000-01-01

    Full Text Available Estudou-se o efeito da redução do nível protéico da dieta, com e sem suplementação enzimática, sobre as características digestivas e composição do conteúdo cecal de coelhos em crescimento. Usou-se um delineamento inteiramente ao acaso, com 54 coelhos da raça Nova Zelândia Branco, de ambos os sexos, desmamados com 35 dias de idade, e divididos em cinco grupos (tratamentos, quatro de 11 e um de 10 coelhos. Os tratamentos constituíram-se de dietas calculadas como isoenergéticas e isoaminoacídicas, com 17, 16, 15 e 14% de proteína bruta (PB, suplementadas com 0,05% de um complexo enzimático comercial (Vegproâ com atividades de protease e celulase e uma dieta controle com 17% PB sem adição do complexo enzimático. Os pesos do trato digestivo cheio, ceco cheio, ceco vazio e conteúdo cecal foram significativamente afetados pelo nível de PB da dieta. Das frações químicas do conteúdo cecal (matéria seca, proteína bruta, fibra em detergente ácido, matéria orgânica e nitrogênio amoniacal, matéria orgânica, nitrogênio amoniacal e proporções molares dos ácidos acético e propiônico foram significativamente afetadas pelo nível de PB da dieta. Os resultados permitem concluir que a redução do nível de PB da dieta para 14 % não influenciou negativamente as características estudadas.

  6. Densification of silica glass at ambient pressure

    International Nuclear Information System (INIS)

    Zheng Lianqing; An Qi; Fu Rongshan; Ni Sidao; Luo, S.-N.

    2006-01-01

    We show that densification of silica glass at ambient pressure as observed in irradiation experiments can be attributed to defect generation and subsequent structure relaxation. In our molecular dynamics simulations, defects are created by randomly removing atoms, by displacing atoms from their nominal positions in an otherwise intact glass, and by assigning certain atom excess kinetic energy (simulated ion implantation). The former forms vacancies; displacing atoms and ion implantation produce both vacancies and 'interstitials'. Appreciable densification is induced by these defects after equilibration of the defective glasses. The structural and vibrational properties of the densified glasses are characterized, displaying resembling features regardless of the means of densification. These results indicate that relaxation of high free-energy defects into metastable amorphous structures enriched in atomic coordination serves as a common mechanism for densification of silica glass at ambient pressure

  7. Next generation intelligent environments ambient adaptive systems

    CERN Document Server

    Nothdurft, Florian; Heinroth, Tobias; Minker, Wolfgang

    2016-01-01

    This book covers key topics in the field of intelligent ambient adaptive systems. It focuses on the results worked out within the framework of the ATRACO (Adaptive and TRusted Ambient eCOlogies) project. The theoretical background, the developed prototypes, and the evaluated results form a fertile ground useful for the broad intelligent environments scientific community as well as for industrial interest groups. The new edition provides: Chapter authors comment on their work on ATRACO with final remarks as viewed in retrospective Each chapter has been updated with follow-up work emerging from ATRACO An extensive introduction to state-of-the-art statistical dialog management for intelligent environments Approaches are introduced on how Trust is reflected during the dialog with the system.

  8. Activity of fungal phytases stored in two ways in response to the period of storage at room temperature Atividade de fitases fúngicas armazenadas de duas maneiras em resposta ao período de armazenamento à temperatura ambiente

    Directory of Open Access Journals (Sweden)

    Luciana de Paula Naves

    2012-01-01

    Full Text Available Two distinct experiments were conducted simultaneously with phytases of Aspergillus oryzae and A. niger for determining enzyme activity in response to storage period (up to 180 days at room temperature - RT. In the first experiment, enzymes were stored as they were acquired (pure form and the activity was measured periodically during 180 days of storage at RT. In the second experiment, the phytases were incorporated to a supplement containing vitamins, minerals, and amino acids; and then this supplement was stored at RT up to 180 days, so that every 30 days of storage was collected one aliquot from each replicate for determining enzymatic activity. In conclusion, the phytase activity is affected by storage duration. To ensure 80% of the initial activity, the phytases of A. oryzae and A. niger can be stored in the pure forms for up to 53 and 135 days at RT, respectively. However, if the phytases of A. oryzae and A. niger are incorporated to a supplement containing vitamins, minerals, and amino acids then the storage period at RT should not exceed 67 and 77 days, respectively.Dois experimentos distintos foram realizados simultaneamente com fitases de Aspergillus oryzae e A. niger para a determinação da atividade enzimática em resposta ao período de armazenamento (por até 180 dias em temperatura ambiente - TA. No primeiro experimento, as enzimas foram armazenadas como adquiridas (forma pura e a atividade foi determinada periodicamente durante 180 dias de armazenamento em TA. No segundo experimento, as fitases foram incorporadas a um suplemento contendo vitaminas, minerais e aminoácidos. Então este suplemento foi armazenado em TA por até 180 dias, de modo que, a cada 30 dias de armazenamento, uma alíquota de cada repetição foi coletada para a determinação da atividade enzimática. Conclui-se que a atividade da fitase é afetada pela duração do armazenamento. Para assegurar 80% da atividade inicial, as fitases de A. oryzae e A. niger

  9. Electric Power From Ambient Energy Sources

    Energy Technology Data Exchange (ETDEWEB)

    DeSteese, John G.; Hammerstrom, Donald J.; Schienbein, Lawrence A.

    2000-10-03

    This report summarizes research on opportunities to produce electric power from ambient sources as an alternative to using portable battery packs or hydrocarbon-fueled systems in remote areas. The work was an activity in the Advanced Concepts Project conducted by Pacific Northwest National Laboratory (PNNL) for the Office of Research and Development in the U.S. Department of Energy Office of Nonproliferation and National Security.

  10. Some measurements of ambient air pollution

    International Nuclear Information System (INIS)

    Memon, H.R.; Memon, A.A.; Behan, M.Y.

    1999-01-01

    Ambient air pollution arising from different sources in Karachi and its surroundings has been studied. The urban centres like Karachi are mostly confronted with eye-irritation, reduce visibility, heart-diseases, nervous disorder, smog and other unpleasant experiences. In this paper quantitative estimations of some air-pollutants such as sulphur dioxide, carbon monoxide, oxides of nitrogen, chlorine and particular matters are presented with their hazardous effects. The remedial measures for the control of major air emissions are also discussed. (author)

  11. ANÁLISE DA QUALIDADE AMBIENTAL URBANA

    Directory of Open Access Journals (Sweden)

    Cíntia Minaki

    2012-01-01

    mostrando que, a pesar de ser una pequeña ciudad, sus características de la ocupación son suficientes para generar una disminución en la calidad ambiental. En este trabajo, también fue posible proponer ideas para mejorar la calidad de estos espacios, desde el punto de vista de la Planificación del Paisaje.

  12. Mass Spectrometry Imaging under Ambient Conditions

    Science.gov (United States)

    Wu, Chunping; Dill, Allison L.; Eberlin, Livia S.; Cooks, R. Graham; Ifa, Demian R.

    2012-01-01

    Mass spectrometry imaging (MSI) has emerged as an important tool in the last decade and it is beginning to show potential to provide new information in many fields owing to its unique ability to acquire molecularly specific images and to provide multiplexed information, without the need for labeling or staining. In MSI, the chemical identity of molecules present on a surface is investigated as a function of spatial distribution. In addition to now standard methods involving MSI in vacuum, recently developed ambient ionization techniques allow MSI to be performed under atmospheric pressure on untreated samples outside the mass spectrometer. Here we review recent developments and applications of MSI emphasizing the ambient ionization techniques of desorption electrospray ionization (DESI), laser ablation electrospray ionization (LAESI), probe electrospray ionization (PESI), desorption atmospheric pressure photoionization (DAPPI), femtosecond laser desorption ionization (fs-LDI), laser electrospray mass spectrometry (LEMS), infrared laser ablation metastable-induced chemical ionization (IR-LAMICI), liquid microjunction surface sampling probe mass spectrometry (LMJ-SSP MS), nanospray desorption electrospray ionization (nano-DESI), and plasma sources such as the low temperature plasma (LTP) probe and laser ablation coupled to flowing atmospheric-pressure afterglow (LA-FAPA). Included are discussions of some of the features of ambient MSI including the ability to implement chemical reactions with the goal of providing high abundance ions characteristic of specific compounds of interest and the use of tandem mass spectrometry to either map the distribution of targeted molecules with high specificity or to provide additional MS information in the structural identification of compounds. We also describe the role of bioinformatics in acquiring and interpreting the chemical and spatial information obtained through MSI, especially in biological applications for tissue

  13. Improved Ambient Pressure Pyroelectric Ion Source

    Science.gov (United States)

    Beegle, Luther W.; Kim, Hugh I.; Kanik, Isik; Ryu, Ernest K.; Beckett, Brett

    2011-01-01

    The detection of volatile vapors of unknown species in a complex field environment is required in many different applications. Mass spectroscopic techniques require subsystems including an ionization unit and sample transport mechanism. All of these subsystems must have low mass, small volume, low power, and be rugged. A volatile molecular detector, an ambient pressure pyroelectric ion source (APPIS) that met these requirements, was recently reported by Caltech researchers to be used in in situ environments.

  14. Münih’te Tüketime Sunulan Koyun ve Keçi Peynirlerinde İnek Sütü Karışımının Enzim-İmmünolojik Sandwich-EIA Testi ile Araştırılması

    Directory of Open Access Journals (Sweden)

    Özer Ergün

    2015-02-01

    Full Text Available Münih piyasasında market ve dükkanlarda satışa sunulan yerli veya ithal koyun keçi peynirlerinden 48 adet toplanmış ve Enzim-immünolojik Sandwich-EIA testi ile inek sütü karışımı yönünden incelenmiştir. Numunelerin 27 tanesinde (%56’da inek sütü karışımı tespit edilmiş olup uygulanan testin hassasiyeti %1’dir.

  15. Variación estacional y potencial enzimático de microhongos asociados con la descomposición de hojarasca de Nothofagus pumilio Seasonal variation and enzymatic potential of microfungi associated with the decomposition of Nothofagus pumilio leaf litter

    OpenAIRE

    EDUARDO VALENZUELA; SERGIO LEIVA; ROBERTO GODOY

    2001-01-01

    Se estudió la variación estacional y el potencial enzimático de microhongos aislados desde el follaje y hojarasca de Nothofagus pumilio sometida a degradación natural durante 1 año. La investigación se realizó en un bosque de N. pumilio ubicado en una microcuenca del Valle de Antillanca, Parque Nacional Puyehue (40° 47' S, 72° 12' O, 1.120 m de altitud). Hojas senescentes colectadas desde árboles de N. pumilio se depositaron en bolsas de malla de nylon ("litter bags") y se sometieron a degrad...

  16. 6th International Symposium on Ambient Intelligence

    CERN Document Server

    Novais, Paulo; Pereira, António; González, Gabriel; Fernández-Caballero, Antonio

    2015-01-01

    This volume contains the proceedings of the 6th International Symposium on Ambient Intelligence (ISAmI 2015), held in Salamanca, Spain on June 3th-5th at the University of Salamanca. After a careful review, 27 papers from 10 different countries were selected to be presented in ISAmI 2015 at the conference and published in the proceedings.  ISAmI has been running annually and aiming to bring together researchers from various disciplines that constitute the scientific field of Ambient Intelligence to present and discuss the latest results, new ideas, projects and lessons learned, namely in terms of software and applications, and aims to bring together researchers from various disciplines that are interested in all aspects of this area. Ambient Intelligence is a recent paradigm emerging from Artificial Intelligence, where computers are used as proactive tools assisting people with their day-to-day activities, making everyone’s life more comfortable. Another main concern of AmI originates from the human comput...

  17. Environment. Chapter 5; Medio ambiente. Capitulo 5

    Energy Technology Data Exchange (ETDEWEB)

    Martin del Castillo, Carlos [Universidad Nacional Autonoma de Mexico, Mexico, D.F. (Mexico)

    2009-07-01

    In this chapter it is mentioned the concern for the care of the environment in Mexico by prominent foreign and Mexican scientists who impelled the creation of a Forest Law. The ecological policies for the conservation of natural resources that cause a sustainable development in Mexico are commented; it is described what the environmental infrastructure consists of; the case of trash handling is analyzed and the Chapter concludes with the relationship of the environment, the climatic change, the infrastructure and the planning. [Spanish] En este capitulo se menciona la preocupacion por el cuidado del medio ambiente en Mexico, por prominentes cientificos extranjeros y mexicanos que impulsaron la creacion de una Ley Forestal. Se comentan las politicas ecologicas para la conservacion de recursos naturales que propicien un desarrollo sustentable en Mexico; se describe en que consiste la infraestructura ambiental; se analiza el caso del manejo de la basura y; se concluye con la relacion del medio ambiente, el cambio climatico, la infraestructura y la planeacion.

  18. LA DIMENSION AMBIENTAL Y LA EDUCACION

    Directory of Open Access Journals (Sweden)

    César Humberto Arias Pabón

    1995-01-01

    Full Text Available El evidente deterioro ambiental al cual se enfrenta nuestra civilización, constituye una seria amenaza para la supervivencia de la especie humana por cuanto afecta sustancialmente la disponibilidad de recursos para mante ner el ritmo de producción de bienes y el avance tecnológico, característicos de la sociedad actual. Tal situación es causada no solamente por un equivocado modelo de desarrollo que considera incompatibles los conceptos de progreso material y conservación, sino por la ignorancia generalizada sobre la dinámica de los diversos procesos ecológicos que de forma natural buscan mantener el equilibrio ambiental del entorno. Ante este fenómeno la Fundación UNIVERSIDAD CENTRAL plantea una estrategia educativa como parte de la solución al problema con el ofrecimiento del programa de Ingeniería en Recursos Hídricos, a través del cual busca integrar la Dimensión Ambiental con la formación universitaria de las juventudes colombianas.

  19. Ambient pressure photoemission spectroscopy of metal surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Baikie, Iain D., E-mail: iain@kptechnology.ltd.uk; Grain, Angela C.; Sutherland, James; Law, Jamie

    2014-12-30

    Highlights: • Ambient pressure photoemission spectroscopy of metals. • Rastered photon energy scan overcomes inelastic scattering. • Relationship between photoemission threshold and contact potential difference. - Abstract: We describe a novel photoemission technique utilizing a traditional Kelvin probe as a detector of electrons/atmospheric ions ejected from metallic surfaces (Au, Ag, Cu, Fe, Ni, Ti, Zn, Al) illuminated by a deep ultra-violet (DUV) source under ambient pressure. To surmount the limitation of electron scattering in air the incident photon energy is rastered rather than applying a variable retarding electric field as is used with UPS. This arrangement can be applied in several operational modes: using the DUV source to determine the photoemission threshold (Φ) with 30–50 meV resolution and also the Kelvin probe, under dark conditions, to measure contact potential difference (CPD) between the Kelvin probe tip and the metallic sample with an accuracy of 1–3 meV. We have studied the relationship between the photoelectric threshold and CPD of metal surfaces cleaned in ambient conditions. Inclusion of a second spectroscopic visible source was used to confirm a semiconducting oxide, possibly Cu{sub 2}O, via surface photovoltage measurements with the KP. This dual detection system can be easily extended to controlled gas conditions, relative humidity control and sample heating/cooling.

  20. Ambiente e justiça: sobre a utilidade do conceito de racismo ambiental no contexto brasileiro

    OpenAIRE

    Silva, Lays Helena Paes e

    2013-01-01

    A ideia de crise ambiental suscita discussões a respeito de distintas concepções de natureza e de ambiente e das disputas simbólicas e materiais neste meio. Uma concepção que toma sociedade e ambiente como pares inseparáveis levou à emergência, nos Estados Unidos, de movimentos que introduziram conceitos como justiça ambiental e racismo ambiental. No Brasil, surgiram movimentos e estudos focados nas mesmas bandeiras de luta e nos mesmos conceitos. Considerando as especificidades do racismo no...

  1. La Educación Ambiental una herramienta clave de la Gestión Ambiental

    Directory of Open Access Journals (Sweden)

    María josé Esteves Ivanissevich

    2013-06-01

    Full Text Available Entendemos que el objeto de la educación ambiental no es el ambiente como tal, sino nuestra relación con él; la multiplicidad y diversidad de objetivos y la necesidad de anclar la intervención en cada contexto específico, hace de ésta una tarea inmensa y compleja. La provincia del Chubut registra desde el año 1999 un historial importante. Éste trabajo, toma lo realizado en la temática de eliminación de bolsas de polietileno en los espacios públicos, de forma interinstitucional y en ámbitos educativo formal y no formal. Con la hipótesis de que la educación ambiental constituye un instrumento de política ambiental preventivo atendiendo los problemas que afecten o pudieran afectar al ambiente; nos planteamos como objetivos: construir una ciudadanía ambientalmente responsable; Impulsar la formación y capacitación de docentes y actores sociales con una mirada ambiental; Contribuir a la conservación ambiental disponiendo de las nuevas tecnologías. La metodología se sustenta en una concepción que hace eje en el aumento de la participación de la sociedad. Desde el ámbito formal se trabajó durante el 2007-2011 con capacitaciones presenciales y a distancia, para docentes de nivel inicial y primario (434 capacitados, en forma conjunta con el Ministerio de Educación. El equipo de trabajo consta de cuatro profesionales del Ministerio de Ambiente y la colaboración de especialistas según el lugar y la temática. En el ámbito no formal se realizaron charlas y talleres con la comunidad (5826 capacitados; comunicación extrainstitucional por medios masivos de comunicación; campañas ambientales, trabajando en forma conjunta con organismos municipales, provinciales, no gubernamentales y privados. Se cubrieron 28 localidades en todo el territorio provincial. Se abarcó un gran número de docentes, quedando el trabajo con directores y supervisores, para pensar en la inclusión de las temáticas como los residuos sólidos urbanos, en el

  2. La economía ambiental y la política ambiental.

    OpenAIRE

    Caraballo, Leonardo Javier

    2007-01-01

    Presentación. Ramos Jiménez, Alfredo Los círculos bolivarianos. El mito de la unidad del pueblo. Arenas, Nelly y Gómez Calcaño, Luis Lo massmediático y las sociedades contemporáneas. Un acercamiento a la relación medios-democracia. Briceño Romero, Ysabel La economía ambiental y la política ambiental. Caraballo, Leonardo Javier Veinte años de democracia en la Argentina. ¿Qué democracia? Kers, Mercedes y Leiras, Santiago C. La ciencia de la política. Bolívar ...

  3. Auditoria Operacional Ambiental: Instrumento para Efetivação do Direito Fundamental ao Meio Ambiente

    Directory of Open Access Journals (Sweden)

    Azor El Achkar

    2011-12-01

    Full Text Available Este artigo tem o objetivo de ressaltar a importância e competência dos tribunais de contas brasileiros em realizar auditorias publicas com enfoque na questão ambiental. Trata dos primórdios da lida com o tema, ressalta o destaque conferido pela Constituição Federal de 1988, evidencia a responsabilidade do Poder Publico com o assunto e observa que as cortes de contas tem missão constitucional de avaliar as acoes empreendidas para preservação e conservação do meio ambiente ecologicamente equilibrado.

  4. Ambient air pollution and semen quality.

    Science.gov (United States)

    Nobles, Carrie J; Schisterman, Enrique F; Ha, Sandie; Kim, Keewan; Mumford, Sunni L; Buck Louis, Germaine M; Chen, Zhen; Liu, Danping; Sherman, Seth; Mendola, Pauline

    2018-05-01

    Ambient air pollution is associated with systemic increases in oxidative stress, to which sperm are particularly sensitive. Although decrements in semen quality represent a key mechanism for impaired fecundability, prior research has not established a clear association between air pollution and semen quality. To address this, we evaluated the association between ambient air pollution and semen quality among men with moderate air pollution exposure. Of 501 couples in the LIFE study, 467 male partners provided one or more semen samples. Average residential exposure to criteria air pollutants and fine particle constituents in the 72 days before ejaculation was estimated using modified Community Multiscale Air Quality models. Generalized estimating equation models estimated the association between air pollutants and semen quality parameters (volume, count, percent hypo-osmotic swollen, motility, sperm head, morphology and sperm chromatin parameters). Models adjusted for age, body mass index, smoking and season. Most associations between air pollutants and semen parameters were small. However, associations were observed for an interquartile increase in fine particulates ≤2.5 µm and decreased sperm head size, including -0.22 (95% CI -0.34, -0.11) µm 2 for area, -0.06 (95% CI -0.09, -0.03) µm for length and -0.09 (95% CI -0.19, -0.06) µm for perimeter. Fine particulates were also associated with 1.03 (95% CI 0.40, 1.66) greater percent sperm head with acrosome. Air pollution exposure was not associated with semen quality, except for sperm head parameters. Moderate levels of ambient air pollution may not be a major contributor to semen quality. Published by Elsevier Inc.

  5. Towards full waveform ambient noise inversion

    Science.gov (United States)

    Sager, Korbinian; Ermert, Laura; Boehm, Christian; Fichtner, Andreas

    2018-01-01

    In this work we investigate fundamentals of a method—referred to as full waveform ambient noise inversion—that improves the resolution of tomographic images by extracting waveform information from interstation correlation functions that cannot be used without knowing the distribution of noise sources. The fundamental idea is to drop the principle of Green function retrieval and to establish correlation functions as self-consistent observables in seismology. This involves the following steps: (1) We introduce an operator-based formulation of the forward problem of computing correlation functions. It is valid for arbitrary distributions of noise sources in both space and frequency, and for any type of medium, including 3-D elastic, heterogeneous and attenuating media. In addition, the formulation allows us to keep the derivations independent of time and frequency domain and it facilitates the application of adjoint techniques, which we use to derive efficient expressions to compute first and also second derivatives. The latter are essential for a resolution analysis that accounts for intra- and interparameter trade-offs. (2) In a forward modelling study we investigate the effect of noise sources and structure on different observables. Traveltimes are hardly affected by heterogeneous noise source distributions. On the other hand, the amplitude asymmetry of correlations is at least to first order insensitive to unmodelled Earth structure. Energy and waveform differences are sensitive to both structure and the distribution of noise sources. (3) We design and implement an appropriate inversion scheme, where the extraction of waveform information is successively increased. We demonstrate that full waveform ambient noise inversion has the potential to go beyond ambient noise tomography based on Green function retrieval and to refine noise source location, which is essential for a better understanding of noise generation. Inherent trade-offs between source and structure

  6. Pedagogía ambiental y didáctica ambiental: tendencias en la educación superior

    Directory of Open Access Journals (Sweden)

    Julio César Tovar-Gálvez

    2017-01-01

    Full Text Available El artículo aborda el problema del currículo de educación ambiental en la educación superior, para lo que se centra en los conceptos pedagogía ambiental y didáctica ambiental. La metodología consiste en realizar un estado del arte para identificar las tendencias existentes sobre pedagogía ambiental y didáctica ambiental, para desde allí diseñar una encuesta que es dirigida a profesores y estudiantes de programas con formación en lo ambiental, de algunas universidades en Bogotá, con el objetivo de identificar sus opiniones como expertos en lo ambiental. Los resultados muestran un especial énfasis en las tendencias sobre la complejidad, pero así mismo plantean la posibilidad de múltiples modelos según los contextos y necesidades.

  7. Arrefecimento evaporativo de ambientes e elementos construtivos

    OpenAIRE

    Vidigal, Filipa Vitória Santana

    2014-01-01

    Dissertação para obtenção do Grau de Mestre em Engenharia Civil – Perfil de Construção A presente dissertação foi preparada no âmbito do Convénio existente entre o Laboratório Nacional de Engenharia Civil (LNEC) e a Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa (FCT-UNL), tendo sido realizada no LNEC. A presente dissertação discute a capacidade dos materiais de construção porosos para promoverem o arrefecimento evaporativo de ambientes. A evaporação pode ser ...

  8. MESIANISMO EN EL PENSAMIENTO AMBIENTAL LATINOAMERICANO

    Directory of Open Access Journals (Sweden)

    Sánchez Buitrago Dairo

    2008-07-01

    Full Text Available Se procura mostrar las formas de autoritarismo simbólico con las cuales las ideologías mesiánicas presentes en el pensamiento ambiental latinoamericano mantienen su cohesión ideológica, evidenciando las configuraciones simbólicas y las acciones que muestran la manera como estas simbologías se observan. Para ello, se argumentará la distinción y la coevolución entre el sentido antropocéntrico y el sentido diferenciado no­antropocéntrico.

  9. Time-series analysis for ambient concentrations

    Energy Technology Data Exchange (ETDEWEB)

    Gonzalez-Manteiga, W.; Prada-Sanchez, J.M.; Cao, R.; Garcia-Jurado, I.; Febrero-Bande, M.; Lucas-Dominguez, T. (Santiago de Compostela University, Santiago de Compostela (Spain). Dept. of Statistics and Operations Research)

    1993-02-01

    In this paper a dynamic system is presented which has been implemented to predict, every 5 min, the ambient concentrations of SO[sub 2] in the neighbourhood of a power station run by ENDESA, the National Electricity Company of Spain, in As Pontes. This prediction task is very important in order to prevent a high ground-level of concentration of SO[sub 2]. For forecasting a mixed model is used which has a parametric component and a nonparametric one. Confidence intervals are also constructed for future observations using bootstrap and classical techniques. 4 refs., 5 figs., 3 tabs.

  10. Sêneca e o ambiente hebraico de Roma

    Directory of Open Access Journals (Sweden)

    José Carlos Silva de Almeida

    2013-05-01

    Full Text Available Enquanto ocupante de um cargo importante no Império Romano do I século, Sêneca certamente conheceu vários dos ensinamentos e práticas da comunidade hebraica de Roma, conforme observamos em alguns trechos de sua obra e de autores posteriores. Se por um lado seu pensamento parece se aproximar da doutrina e prática hebraicas, embora por razões diversas, por outro lado se afasta. Todavia ele soube, como nenhum outro escritor latino, manifestar o devido respeito e reserva ao ambiente hebraico da Urbs.

  11. Las cuentas nacionales y el medio ambiente

    Directory of Open Access Journals (Sweden)

    J. A. Torres Pérez

    2001-01-01

    Full Text Available El Sistema de Cuentas Nacionales (SCN vigente, medido a través del Producto Interno Bruto (PIB soslaya todos los activos ambientales, así como su deterioro y depreciación de los recursos naturales por lo que refleja tasas que sobreestiman el crecimiento económico En el presente documento se discute sobre el SCN, sus limitaciones para cuantificar el deterioro del capital natural e impacto ambiental. Se presentan las experiencias desarrolladas en otros países y se promueve la posibilidad de desarrollar un sistema de cuentas nacionales en México que incluya los recursos naturales.

  12. Reflexo do consumismo infantil no ambiente escolar

    OpenAIRE

    Camargo, Sônia de Fátima; Vieira Júnior, Hélio

    2011-01-01

    Este artigo propôs verificar se há reflexos do consumismo infantil no ambiente escolar. Se á fatores que influenciam nas interações afetivas e subjetivas dos alunos das séries iniciais do Ensino Fundamental na Escola Municipal Professora Ana Cristina de sena município de Sinop – MT. O objetivo do trabalho foi investigar as relações interpessoais em sala de aula e de que forma essas relações acontecem diante do consumismo. Como fundamentação teórica, recorremos aos autores, Zygmunt Bauman, Ann...

  13. Cognitive assisted living ambient system: a survey

    Directory of Open Access Journals (Sweden)

    Ruijiao Li

    2015-11-01

    Full Text Available The demographic change towards an aging population is creating a significant impact and introducing drastic challenges to our society. We therefore need to find ways to assist older people to stay independently and prevent social isolation of these population. Information and Communication Technologies (ICT provide various solutions to help older adults to improve their quality of life, stay healthier, and live independently for a time. Ambient Assisted Living (AAL is a field to investigate innovative technologies to provide assistance as well as healthcare and rehabilitation to impaired seniors. The paper provides a review of research background and technologies of AAL.

  14. Ambientes de aprendizaje en el siglo xxi

    OpenAIRE

    Correa, PhD Francisco Jose

    2010-01-01

    El papel de la educación en la sociedad ha cambiado debido al surgimiento de paradigmas y nuevas mediaciones que exigen las tecnologías de la información y la comunicación. Los ambientes de aprendizaje, entendidos como los espacios en los cuales se desarrollan los procesos de enseñanza y de aprendizaje, también están evolucionando de forma rápida y efectiva. Las nuevas generaciones son afectadas cada vez desde más temprana edad, por la inclusión de las diferentes tecnologías en su vida cotidi...

  15. 7th Ambient Assisted Living Congress

    CERN Document Server

    Klausing, Helmut

    2015-01-01

    In this book, leading authors in the field discuss developments of Ambient Assisted Living. The contributions have been chosen and invited at the 7th AAL congress, Berlin. It presents new technological developments which support the autonomy and independence of individuals with special needs. As the technological innovation raises also social issues, the book addresses micro and macro economical aspects of assistive systems and puts an additional emphasis on the ethical and legal discussion. The presentation is supported by real world examples and applications.

  16. Memory Tests with Ambient Odours "Make Scents"

    OpenAIRE

    Nord, Marie

    2015-01-01

    An ambient odour of anise was used in a context-dependent memory study with three different memory tasks targeting both declarative and non-declarative memory functions. Declarative memory was assessed by means of two episodic memory tests; recall of a prose text and a complex figure. Priming was used to assess the non-declarative memory with word fragment completion. Memory was tested immediately and after 48 hours. The results showed a significant main effect of context (odour or not) for a...

  17. Emprego de reação imuno-enzimática na determinação do caráter genético de amostras de poliovírus Use of an immunoenzymatic reaction in the determination of the genetic character of poliovirus samples

    Directory of Open Access Journals (Sweden)

    Hermann G. Schatzmayr

    1985-03-01

    Full Text Available Uma reação imuno-enzimática em microplacas foi utilizada para a diferenciação do caráter genético de amostras de poliovirus, em relação às amostras padrões atenuadas de Sabin tipos 1,2 e 3 e às amostrwas virulentas Mahoney, MEF- 1 e Saukett, utilizando soros policlonais mono-específicos, preparados por absorção com as amostras heterólogas de cada tipo. Em 74 das 79 amostras de vírus examinadas (93,6% foi possivel identificar o seu caráter como amostras selvagens ou vacinais. A utilização da prova imuno-enzimática aplicada aos vírus da poliomielite é discutida.Characterization of poliovirus strains by an enzyme immunoassay (EIA. An enzyme immunoassay performed with polyclonal antisera to poliovirus types 1, 2 and 3 absorbed with heterologous strains of the same types was used for the characterization of poliovirus field isolates. The assay allowed the identification of 74 (93.6% out of 79 isolates as wild or vaccine-like strains. Applications of EIA for the study of polioviruses are discussed.

  18. Influência do tamanho das partículas na solubilização enzimática da proteína do farelo de soja - DOI: 10.4025/actascitechnol.v31i2.696

    Directory of Open Access Journals (Sweden)

    Maura Stenzel

    2009-06-01

    Full Text Available O objetivo deste trabalho foi verificar a influência do tamanho das partículas na quantidade de proteína solubilizada a partir do farelo de soja. O farelo foi peneirado e cada fração foi submetida à hidrólise enzimática com as proteases Alcalase e Flavourzyme. As hidrólises foram realizadas a 60ºC, agitação de 100 rpm, concentração de Alcalase 0,5% (proteína/proteína por 3h e concentração de Flavourzyme 1% (proteína/proteína por 5h. A quantidade de proteína solubilizada foi determinada pelo método de Lowry. A análise granulométrica do farelo mostrou que quase metade da amostra corresponde a partículas maiores que 1,68 mm. O modelo que resultou em melhor ajuste dos dados foi o GGS, e o diâmetro médio de Sauter da amostra foi 1,150 mm. Com relação à hidrólise enzimática, para as duas enzimas foi verificada uma tendência de aumento na quantidade solubilizada para as frações mais finas e maior efetividade da Alcalase na solubilização proteica.

  19. Ambient versus traditional environment in pediatric emergency department.

    Science.gov (United States)

    Robinson, Patricia S; Green, Jeanette

    2015-01-01

    We sought to examine the effect of exposure to an ambient environment in a pediatric emergency department. We hypothesized that passive distraction from ambient lighting in an emergency department would lead to reduction in patient pain and anxiety and increased caregiver satisfaction with services. Passive distraction has been associated with lower anxiety and pain in patients and affects perception of wait time. A pediatric ED was designed that optimized passive distraction techniques using colorful ambient lighting. Participants were nonrandomly assigned to either an ambient ED environment or a traditional ED environment. Entry and exit questionnaires assessed caregiver expectations and experiences. Pain ratings were obtained with age-appropriate scales, and wait times were recorded. A total of 70 participants were assessed across conditions, that is, 40 in the ambient ED group and 30 in the traditional ED group. Caregivers in the traditional ED group expected a longer wait, had higher anxiety pretreatment, and felt more scared than those in the ambient ED group. Caregivers in the ambient ED group felt more included in the care of their child and rated quality of care higher than caregivers in the traditional ED group. Pain ratings and administrations of pain medication were lower in the ambient ED group. Mean scores for the ambient ED group were in the expected direction on several items measuring satisfaction with ED experiences. Results were suggestive of less stress in caregivers, less pain in patients, and higher satisfaction levels in the ambient ED group. © The Author(s) 2015.

  20. Ambient Noise in an Urbanized Tidal Channel

    Science.gov (United States)

    Bassett, Christopher

    In coastal environments, when topographic and bathymetric constrictions are combined with large tidal amplitudes, strong currents (> 2 m/s) can occur. Because such environments are relatively rare and difficult to study, until recently, they have received little attention from the scientific community. However, in recent years, interest in developing tidal hydrokinetic power projects in these environments has motivated studies to improve this understanding. In order to support an analysis of the acoustic effects of tidal power generation, a multi-year study was conducted at a proposed project site in Puget Sound (WA) are analyzed at a site where peak currents exceeded 3.5 m/s. From these analyses, three noise sources are shown to dominate the observed variability in ambient noise between 0.02-30 kHz: anthropogenic noise from vessel traffic, sediment-generated noise during periods of strong currents, and flow-noise resulting from turbulence advected over the hydrophones. To assess the contribution of vessel traffic noise, one calendar year of Automatic Identification System (AIS) ship-traffic data was paired with hydrophone recordings. The study region included inland waters of the Salish Sea within a 20 km radius of the hydrophone deployment site in northern Admiralty Inlet. The variability in spectra and hourly, daily, and monthly ambient noise statistics for unweighted broadband and M-weighted sound pressure levels is driven largely by vessel traffic. Within the one-year study period, at least one AIS transmitting vessel is present in the study area 90% of the time and over 1,363 unique vessels are recorded. A noise budget for vessels equipped with AIS transponders identifies cargo ships, tugs, and passenger vessels as the largest contributors to noise levels. A simple model to predict received levels at the site based on an incoherent summation of noise from different vessel types yields a cumulative probability density function of broadband sound pressure

  1. Caracterização físico-química e enzimática de uva 'Patricia' cultivada na região de Primavera do Leste - MT Physical chemistry and enzymatic characterization of grape 'Patricia' cultivated in Primavera do Leste - MT

    Directory of Open Access Journals (Sweden)

    Merce Teodora Aguil Santana

    2008-02-01

    Full Text Available Amostras de uva (Vitis labrusca L. cv Patrícia, produzidos na região de Primavera do Leste no Estado de Mato Grosso, foram analisadas pelos parâmetros químicos segundo a legislação: pH, sólidos solúveis (ºBrix, acidez titulável, vitamina C e atividade enzimática das enzimas polifenoloxidase (PPO; peroxidase (POD; pectinametilesterase (PME; e poligalacturonase (PG. O valor de sólidos solúveis (SS das amostras analisadas foi de 17,69 ºBrix. O valor de pH encontrado foi de 3,69. A acidez titulável (AT apresentou valor de 0,8 g de ácido tartárico/100 mL de suco. O valor de vitamina C foi de 17,54 mg/mL. A atividade enzimática da polifenoloxidase (PPO foi de 22,15 U/g/min. As atividades enzimáticas para as enzimas peroxidase (POD e poligalacturonase apresentaram valores de: 11,59 U/g/min e 3778,61 U/g/min, respectivamente. Não foram detectados valores para a atividade enzimática da pectinametilesterase (PME, nas amostras analisadas. Os parâmetros químicos analisados se encontram dentro dos padrões estabelecidos pela legislação. Pelos resultados apresentados a cultivar Patrícia apresenta um valor elevado de vitamina C e, quando em estado de maturação, apresenta valores elevados da atividade enzimática da poligalacturonase (PG.Grape samples (Vitis labrusca L. 'Patrícia', produced in the area of Primavera do Leste in the State of Mato Grosso, were analyzed by the chemical parameters according to the legislation: pH, soluble solids (ºBrix, titrable acidity, vitamin C and enzymatic activity of the polyfenoloxidase (PPO; peroxydase (POD; pectinmetylesterase (PME; and polygalacturonase (PG. The value of soluble solids (SS of the analyzed samples it was of 17.69 ºBrix. The value of found pH was of 3.69. The titrable acidity (AT showed value of 0.8 g of acid tartaric/100 mL juice. The vitamin C value was 17.54 mg/mL. The enzymatic activity of the polyfenoloxidase (PPO was 22.15 U/g/min. The enzymatic activities for the enzymes

  2. Efecto de los extractos acuoso e hidroetanólico de hojas de Bixa orellana (achiote sobre los indicadores no enzimáticos de la hepatotoxicidad por paracetamol, en ratas

    Directory of Open Access Journals (Sweden)

    Oscar Huamán

    2013-10-01

    Full Text Available Objetivos: Determinar el efecto de los extractos acuoso e hidroetanólico de las hojas de Bixa orellana sobre los indicadores no enzimáticos de hepatotoxicidad, en ratas sometidas a paracetamol. Diseño: Experimental. Institución: Centro de Investigación de Bioquímica y Nutrición, Universidad Nacional Mayor de San Marcos, Lima, Perú. Material biológico: Ratas machos de 3 meses de edad. Intervenciones: Se distribuyó aleatoriamente 35 ratas machos de 3 meses de edad en 5 grupos, que recibieron vía peroral por 10 días: NaCl 0,9% los controles positivo y negativo, silimarina 300 mg/kg, extracto acuoso 500 mg/kg y extracto hidroetanólico 500 mg/kg. Previo ayuno de 24 horas, al quinto día se administró paracetamol (400 mg/kg peroral, excepto al control negativo. Bajo anestesia con éter, se realizó punción cardiaca para extraer sangre. Principales medidas de resultados: Ratio hepático (peso hígado/peso animal x 100, bilirrubina total (BT, directa (BD e indirecta (BI, hepatomegalia, especies reactivas al ácido tiobarbitúrico (TBARS en hígado y suero. Resultados: El tratamiento con extracto acuoso solo disminuyó los indicadores BT y BI (p<0,01, TBARS en suero (p<0,05 y hubo disminución de la masa hepática de -13,2% (p<0,01. En el grupo que recibió el extracto hidroetanólico se redujo la BT, BI (p<0,01, BD (p<0,05, TBARS en hígado (p<0,01 y la masa hepática -9,37%. Conclusiones: Los extractos acuoso e hidroetanólico de hojas de Bixa orellana (achiote presentarían efecto hepatoprotector frente al paracetamol a dosis tóxica, en ratas.

  3. Determinación de antioxidantes enzimáticos en variedades e híbridos de Morus alba Determination of enzymatic antioxidants in Morus alba varieties and hybrids

    Directory of Open Access Journals (Sweden)

    2010-09-01

    Full Text Available En el presente trabajo se evaluó la actividad específica de las enzimas antioxidantes catalasa y guaiacol peroxidasa en extractos frescos de variedades e híbridos de morera (Morus alba, obtenidos a partir de las raíces, los tallos y las hojas. Las muestras de los diferentes órganos en estudio fueron colectadas aleatoriamente, maceradas en nitrógeno líquido, resuspendidas en tampón fosfato de sodio y centrifugadas. El sobrenadante se utilizó para las determinaciones enzimáticas. Se empleó el ANOVA de clasificación simple y la prueba de rangos múltiples de Duncan para la comparación de medias (PIn this work the specific activity of the antioxidant enzymes catalase and guaiacol peroxidase was evaluated in fresh extracts of mulberry (Morus alba varieties and hybrids, obtained from the roots, stems and leaves. The samples of the different organs under study were randomly collected, macerated in liquid nitrogen, resuspended in sodium phosphate buffer and centrifuged. The supernatant was used for the enzymatic determinations. The simple classification ANOVA and Duncan´s multiple range test were used for mean comparison (P<0,05. The highest values of specific catalase and peroxidase activity were found, in general, in the leaves, followed by stems and roots. The varieties and hybrids with higher specific activity for both enzymes were: Tigreada, Criolla, IZ 15-9 and IZ 64. The results showed the protective antioxidant role of the extracts, mainly from leaves, from the important functions played by these enzymatic systems in the elimination of reactive oxygen species. The potential of this species for animal nutrition and health is corroborated, which gives it more value as a multipurpose forage plant. It is recommended to continue making other determinations of compounds with antioxidant activities and studying the catalase and peroxidase enzymatic systems in multipurpose species, such as M. alba, used in animal feeding and traditional

  4. Comparación de los métodos de inmunoensayo enzimático automatizado (VIDAS y PCR para la detección de Salmonella spp. en expendios de la ciudad de Santa Marta (Colombia

    Directory of Open Access Journals (Sweden)

    Libardo Acosta

    2013-01-01

    Full Text Available Objetivo: comparar los métodos de inmunoensayo enzimático automatizado VIDAS, (Vi- tek Immunodiagnostic Assay System, y el análisis molecular por Reacción en Cadena de la Polimerasa (PCR para detectar Salmonella spp en expendios de la ciudad de Santa Marta (Colombia. Materiales y métodos: Previamente se reportó la estandarización de la técnica de PCR para la detección de Salmonella spp. en un lapso de 12 horas. En colaboración con el Labo- ratorio de Salud Pública del departamento del Magdalena se procedió a tomar un total de 65 muestras de alimentos para análisis de vigilancia rutinaria, discriminados así: carne de res: 14 (21.5 %, embutidos: 18 (27.7 %, pollo: 7(10.8 %, pescado: 3(4.6 %, harinas: 13 (20 %, lácteos: 5 (7.7 % salsas: 4(6.2 % y ensalada: 1(1.5 %, en la ciudad de Santa Marta entre septiembre y noviembre de 2010. Las 65 muestras fueron sometidas a análisis microbiológico para la determinación de Salmonella spp. en el Laboratorio de Salud Pú- blica del departamento del Magdalena siguiendo el protocolo del VIDAS. Una alícuota de 1 mL del preenriquecimiento no selectivo fue enviada refrigerada al laboratorio de biología molecular de la Universidad Cooperativa de Colombia para ser analizada por PCR. Resultados: Los resultados muestran que los alimentos analizados en el Laboratorio de Salud Pública del departamento del Magdalena con VIDAS presentaron Salmonella spp. únicamente en cárnicos: 5/65 (7.7 %, mientras que esas mismas muestras analizadas por PCR mostraban la presencia de Salmonella spp. en 36/65 (55.4 %. Conclusiones: Los resultados indican que la PCR puede ser aplicada para realizar vigilan- cia epidemiológica y obtener resultados cualitativos confiables en menor tiempo.

  5. Producción de lípidos estructurados por transesterificación enzimática del aceite de soja y aceite de palmiste en reactor de lecho empacado

    Directory of Open Access Journals (Sweden)

    Perea Villamil, Aide

    2008-12-01

    Full Text Available Enzymatic synthesis of structured lipids by transesterification of soybean oil with palm kernel oil was evaluated in a packed-bed reactor with a capacity for 500g of enzyme loading. Lipozyme RM-IM was used as catalyst. Substrate blends were passed through the enzyme bed at different flow rates. Transesterification reached a level of 19.6 %, with a maximum calculated productivity of 2344 kg of transesterified oil/kg of immobilized enzyme, a flow rate of 9,36 kg oil/kg enzyme/h. The triacylglycerols formed in major proportion were C40:2, C42:2, C42:3, C44:2, C44:3, C50:3 and C50:4. Stereoespecific analysis of the fat before and after transesterification shows a slight migration of acyl groups. The products obtained by this technology can be applied in the formulation of lipid emulsions for enteral and parenteral nutrition and the food industry.Se evaluó la síntesis enzimática de lípidos estructura-dos por transesterificación de aceite de soja con aceite de palmiste en un reactor de lecho empacado (PBR con capacidad para 500 gramos de enzima, utilizando como catalizador Lipozyme RM-IM. La mezcla de sustratos se hizo pasar a través del lecho de enzima a 70 °C y diferentes flujos de aceite. A un flujo de 9.36 kg aceite/kg enzima/h se alcanzó un grado transesterificación de 19.6 % con una productividad máxima calculada de 2344 kg aceite/kg enzima. Los triacilgliceroles que se formaron en mayor proporción fueron el C40:2, C42:2, C42:3, C44:2, C44:3, C50:3 y C50:4. El análisis estereoespecífico de la mezcla grasa antes y después de la transesterificación indicó baja migración de grupos acilo. Los productos obtenidos pueden tener aplicación en la formulación de emulsiones lípidicas para nutrición enteral y parenteral y en la industria de alimentos.

  6. Efeito da suplementação enzimática no desempenho e qualidade dos ovos de poedeiras comerciais = Effects of the enzyme complex supplementation on the performance and egg quality of laying hens

    Directory of Open Access Journals (Sweden)

    Alice Eiko Murakami

    2007-04-01

    Full Text Available O objetivo deste trabalho foi determinar o efeito da utilização de um complexo multienzimático sobre o desempenho e qualidade dos ovos de poedeiras comerciais. Foram utilizadas 336 poedeiras comerciais, HyLine W36, com 29 semanas de idade distribuídas em um delineamento inteiramente casualizado com 7 tratamentos e 6 repetições de 8 aves cada. Os tratamentos consistiram de uma dieta basal suplementada com o complexo enzimático nos níveis de 400 ou 500 ppm com valorização do nível de proteína, energia metabolizável e aminoácidos do farelo de soja em 5/5/5, 7/7/7 e 7/7/9%, respectivamente. As variáveisestudadas foram: consumo de ração (g ave-1 dia-1, conversão alimentar (kg dz-1 de ovos, peso médio dos ovos (g, produção de ovos (%, gravidade específica (g mL-1, Unidade Haugh, coloração da gema, percentuais de sólidos totais, de gema, clara e casca (%,espessura da casca (mm e composição bromatológica das excretas. A adição do complexo multienzimático apresentou resultados produtivos e de qualidade dos ovos semelhantes à dieta controle.The present study aimed to evaluate the effects of a commercial enzyme complex on the performance and egg quality of commerciallaying hens. A total of 336 29-week old Hyline W36 layers were allocated to a completely randomized experimental design with seven treatments and six replications of eight birds each. The treatments consisted of the basal diet supplemented with inclusion of (500 or 400ppm, overestimating the percentages of protein, ME and amino acids of the soybean meal by 5:5:5, 7:7:7 and 7:7:9, respectively. The studied variables were: feed intake (g bird-1 day-1, feed:egg (kg feed-1 egg dozen-1, egg weight (g, egg laying percentage (hen day-1 specific gravity (g ml-1, Haugh Unit, color of the egg yolk, total solids percentage (%, egg yolk percentage, albumen and eggshell (%, eggshell thickness (mm and chemical compositionof hen manure. The inclusion of enzyme complex in the

  7. Pedagogía ambiental y didáctica ambiental como fundamentos del currículo para la formación ambiental

    OpenAIRE

    Julio César Tovar-Gálvez

    2013-01-01

    Se trata el problema de la fundamentación del currículo para la formación ambiental en la educación superior, frente a lo cual es necesario construir los conceptos de pedagogía ambiental y de didáctica ambiental. El marco teórico permite definir las categorías "lo pedagógico" y "lo didáctico", a partir de lo cual se analiza la literatura y con ello definir pedagogía ambiental y didáctica ambiental. El estado del arte permite establecer que existen dos sustentos teóricos: a) uno que se cimenta...

  8. Disseminating Ambient Assisted Living in Rural Areas

    Science.gov (United States)

    Leitner, Gerhard; Felfernig, Alexander; Fercher, Anton J.; Hitz, Martin

    2014-01-01

    The smart home, ambient intelligence and ambient assisted living have been intensively researched for decades. Although rural areas are an important potential market, because they represent about 80% of the territory of the EU countries and around 125 million inhabitants, there is currently a lack of applicable AAL solutions. This paper discusses the theoretical foundations of AAL in rural areas. This discussion is underlined by the achievements of the empirical field study, Casa Vecchia, which has been carried out over a four-year period in a rural area in Austria. The major goal of Casa Vecchia was to evaluate the feasibility of a specific form of AAL for rural areas: bringing AAL technology to the homes of the elderly, rather than moving seniors to special-equipped care facilities. The Casa Vecchia project thoroughly investigated the possibilities, challenges and drawbacks of AAL related to this specific approach. The findings are promising and somewhat surprising and indicate that further technical, interactional and socio-psychological research is required to make AAL in rural areas reasonable in the future. PMID:25068862

  9. A smart kitchen for ambient assisted living.

    Science.gov (United States)

    Blasco, Rubén; Marco, Álvaro; Casas, Roberto; Cirujano, Diego; Picking, Richard

    2014-01-17

    The kitchen environment is one of the scenarios in the home where users can benefit from Ambient Assisted Living (AAL) applications. Moreover, it is the place where old people suffer from most domestic injuries. This paper presents a novel design, implementation and assessment of a Smart Kitchen which provides Ambient Assisted Living services; a smart environment that increases elderly and disabled people's autonomy in their kitchen-related activities through context and user awareness, appropriate user interaction and artificial intelligence. It is based on a modular architecture which integrates a wide variety of home technology (household appliances, sensors, user interfaces, etc.) and associated communication standards and media (power line, radio frequency, infrared and cabled). Its software architecture is based on the Open Services Gateway initiative (OSGi), which allows building a complex system composed of small modules, each one providing the specific functionalities required, and can be easily scaled to meet our needs. The system has been evaluated by a large number of real users (63) and carers (31) in two living labs in Spain and UK. Results show a large potential of system functionalities combined with good usability and physical, sensory and cognitive accessibility.

  10. A Smart Kitchen for Ambient Assisted Living

    Directory of Open Access Journals (Sweden)

    Rubén Blasco

    2014-01-01

    Full Text Available The kitchen environment is one of the scenarios in the home where users can benefit from Ambient Assisted Living (AAL applications. Moreover, it is the place where old people suffer from most domestic injuries. This paper presents a novel design, implementation and assessment of a Smart Kitchen which provides Ambient Assisted Living services; a smart environment that increases elderly and disabled people’s autonomy in their kitchen-related activities through context and user awareness, appropriate user interaction and artificial intelligence. It is based on a modular architecture which integrates a wide variety of home technology (household appliances, sensors, user interfaces, etc. and associated communication standards and media (power line, radio frequency, infrared and cabled. Its software architecture is based on the Open Services Gateway initiative (OSGi, which allows building a complex system composed of small modules, each one providing the specific functionalities required, and can be easily scaled to meet our needs. The system has been evaluated by a large number of real users (63 and carers (31 in two living labs in Spain and UK. Results show a large potential of system functionalities combined with good usability and physical, sensory and cognitive accessibility.

  11. A Versatile Integrated Ambient Ionization Source Platform

    Science.gov (United States)

    Ai, Wanpeng; Nie, Honggang; Song, Shiyao; Liu, Xiaoyun; Bai, Yu; Liu, Huwei

    2018-04-01

    The pursuit of high-throughput sample analysis from complex matrix demands development of multiple ionization techniques with complementary specialties. A versatile integrated ambient ionization source (iAmIS) platform is proposed in this work, based on the idea of integrating multiple functions, enhancing the efficiency of current ionization techniques, extending the applications, and decreasing the cost of the instrument. The design of the iAmIS platform combines flowing atmospheric pressure afterglow (FAPA) source/direct analysis in real time (DART), dielectric barrier discharge ionization (DBDI)/low-temperature plasma (LTP), desorption electrospray ionization (DESI), and laser desorption (LD) technique. All individual and combined ionization modes can be easily attained by modulating parameters. In particular, the FAPA/DART&DESI mode can realize the detection of polar and nonpolar compounds at the same time with two different ionization mechanisms: proton transfer and charge transfer. The introduction of LD contributes to the mass spectrometry imaging and the surface-assisted laser desorption (SALDI) under ambient condition. Compared with other individual or multi-mode ion source, the iAmIS platform provides the flexibility of choosing different ionization modes, broadens the scope of the analyte detection, and facilitates the analysis of complex samples. [Figure not available: see fulltext.

  12. Disseminating Ambient Assisted Living in Rural Areas

    Directory of Open Access Journals (Sweden)

    Gerhard Leitner

    2014-07-01

    Full Text Available The smart home, ambient intelligence and ambient assisted living have been intensively researched for decades. Although rural areas are an important potential market, because they represent about 80% of the territory of the EU countries and around 125 million inhabitants, there is currently a lack of applicable AAL solutions. This paper discusses the theoretical foundations of AAL in rural areas. This discussion is underlined by the achievements of the empirical field study, Casa Vecchia, which has been carried out over a four-year period in a rural area in Austria. The major goal of Casa Vecchia was to evaluate the feasibility of a specific form of AAL for rural areas: bringing AAL technology to the homes of the elderly, rather than moving seniors to special-equipped care facilities. The Casa Vecchia project thoroughly investigated the possibilities, challenges and drawbacks of AAL related to this specific approach. The findings are promising and somewhat surprising and indicate that further technical, interactional and socio-psychological research is required to make AAL in rural areas reasonable in the future.

  13. A Smart Kitchen for Ambient Assisted Living

    Science.gov (United States)

    Blasco, Rubén; Marco, Álvaro; Casas, Roberto; Cirujano, Diego; Picking, Richard

    2014-01-01

    The kitchen environment is one of the scenarios in the home where users can benefit from Ambient Assisted Living (AAL) applications. Moreover, it is the place where old people suffer from most domestic injuries. This paper presents a novel design, implementation and assessment of a Smart Kitchen which provides Ambient Assisted Living services; a smart environment that increases elderly and disabled people's autonomy in their kitchen-related activities through context and user awareness, appropriate user interaction and artificial intelligence. It is based on a modular architecture which integrates a wide variety of home technology (household appliances, sensors, user interfaces, etc.) and associated communication standards and media (power line, radio frequency, infrared and cabled). Its software architecture is based on the Open Services Gateway initiative (OSGi), which allows building a complex system composed of small modules, each one providing the specific functionalities required, and can be easily scaled to meet our needs. The system has been evaluated by a large number of real users (63) and carers (31) in two living labs in Spain and UK. Results show a large potential of system functionalities combined with good usability and physical, sensory and cognitive accessibility. PMID:24445412

  14. The influence of ambient light on the driver

    Science.gov (United States)

    Klinger, Karsten D.; Lemmer, Uli

    2008-04-01

    Increasingly, cars are fitted with interior ambient lighting which is switched on while driving. This special kind of interior light emphasizes the interior design of the car, it makes a car look special and gives the buyers a new option to personalize their automobiles. But how does ambient interior light influence the driver? We conducted a series of over 50 tests to study the influence of interior ambient light on contrast perception under different illumination levels, colors and positions of the illuminated areas. Our tests show that in many cases the ambient lighting can improve the visual contrast for seeing objects in the headlamp beam. But the test persons mentioned that the tested brightness looked too bright and that they felt glared. The measured values instead proved that no disability glare exists. Therefore, provided that the drivers can adjust the intensity of the ambient light to avoid glare, the ambient light has no negative effect on the drivers' contrast perception.

  15. Ambient workplace heterosexism: Implications for sexual minority and heterosexual employees.

    Science.gov (United States)

    Miner, Kathi N; Costa, Paula L

    2018-06-01

    This study examined the relationship between ambient workplace heterosexism, emotional reactions (i.e., fear and anger), and outcomes for sexual minority and heterosexual employees. Five hundred thirty-six restaurant employees (68% female, 77% White) completed an online survey assessing the variables of interest. Results showed that greater experiences of ambient workplace heterosexism were associated with heightened fear and anger and, in turn, with heightened psychological distress (for fear) and greater physical health complaints, turnover intentions, and lowered job satisfaction (for anger). Fear also mediated the relationship between ambient workplace heterosexism and psychological distress. In addition, sexual orientation moderated the relationship between ambient workplace heterosexism and fear such that sexual minority employees reported more fear than heterosexuals with greater ambient heterosexism. These effects occurred after controlling for personal experiences of interpersonal discrimination. Our findings suggest that ambient workplace heterosexism can be harmful to all employees, not only sexual minorities or targeted individuals. Copyright © 2018 John Wiley & Sons, Ltd.

  16. Trends in ambient intelligent systems the role of computational intelligence

    CERN Document Server

    Khan, Mohammad; Abraham, Ajith

    2016-01-01

    This book demonstrates the success of Ambient Intelligence in providing possible solutions for the daily needs of humans. The book addresses implications of ambient intelligence in areas of domestic living, elderly care, robotics, communication, philosophy and others. The objective of this edited volume is to show that Ambient Intelligence is a boon to humanity with conceptual, philosophical, methodical and applicative understanding. The book also aims to schematically demonstrate developments in the direction of augmented sensors, embedded systems and behavioral intelligence towards Ambient Intelligent Networks or Smart Living Technology. It contains chapters in the field of Ambient Intelligent Networks, which received highly positive feedback during the review process. The book contains research work, with in-depth state of the art from augmented sensors, embedded technology and artificial intelligence along with cutting-edge research and development of technologies and applications of Ambient Intelligent N...

  17. socio-ec(h)o: Ambient Intelligence and Gameplay

    OpenAIRE

    Wakkary, Ron

    2005-01-01

    The socio-ec(h)o project aims to research a generalized ambient intelligent software platform and design models for responsive environments based on the concept of ambient intelligent "ecologies" and group gameplay. The benefits of the research include a software-architecture, ambient intelligence inference engine, and interaction design models for gameplay and responsive environments. The paper will discuss the results of our prototypes for games in responsive environments. These prototypes ...

  18. Improving Energy Saving Techniques by Ambient Intelligence Scheduling

    DEFF Research Database (Denmark)

    Cristani, Matteo; Karafili, Erisa; Tomazzoli, Claudio

    2015-01-01

    Energy saving is one of the most challenging aspects of modern ambient intelligence technologies, for both domestic and business usages. In this paper we show how to combine Ambient Intelligence and Artificial Intelligence techniques to solve the problem of scheduling a set of devices under a given...... for Ambient Intelligence to a specific framework and exhibit a sample usage for a real life system, Elettra, that is in use in an industrial context....

  19. The Potential for Ambient Plasma Wave Propulsion

    Science.gov (United States)

    Gilland, James H.; Williams, George J.

    2016-01-01

    A truly robust space exploration program will need to make use of in-situ resources as much as possible to make the endeavor affordable. Most space propulsion concepts are saddled with one fundamental burden; the propellant needed to produce momentum. The most advanced propulsion systems currently in use utilize electric and/or magnetic fields to accelerate ionized propellant. However, significant planetary exploration missions in the coming decades, such as the now canceled Jupiter Icy Moons Orbiter, are restricted by propellant mass and propulsion system lifetimes, using even the most optimistic projections of performance. These electric propulsion vehicles are inherently limited in flexibility at their final destination, due to propulsion system wear, propellant requirements, and the relatively low acceleration of the vehicle. A few concepts are able to utilize the environment around them to produce thrust: Solar or magnetic sails and, with certain restrictions, electrodynamic tethers. These concepts focus primarily on using the solar wind or ambient magnetic fields to generate thrust. Technically immature, quasi-propellantless alternatives lack either the sensitivity or the power to provide significant maneuvering. An additional resource to be considered is the ambient plasma and magnetic fields in solar and planetary magnetospheres. These environments, such as those around the Sun or Jupiter, have been shown to host a variety of plasma waves. Plasma wave propulsion takes advantage of an observed astrophysical and terrestrial phenomenon: Alfven waves. These are waves that propagate in the plasma and magnetic fields around and between planets and stars. The generation of Alfven waves in ambient magnetic and plasma fields to generate thrust is proposed as a truly propellantless propulsion system which may enable an entirely new matrix of exploration missions. Alfven waves are well known, transverse electromagnetic waves that propagate in magnetized plasmas at

  20. Glucosilación no enzimática y complicaciones crónicas de la diabetes mellitus Non-enzymatic glycosylation and chronic complications of diabetes mellitus

    Directory of Open Access Journals (Sweden)

    Jeddú Cruz Hernández

    2010-08-01

    Full Text Available INTRODUCCIÓN: la hiperglucemia es considerada hoy como un factor patogénico fundamental del desarrollo de las complicaciones neurovasculares diabéticas y, específicamente, desempeña un papel preponderante en el fenómeno de la glucosilación no enzimática y la formación de productos finales de la glucosilación avanzada. OBJETIVOS: describir los mecanismos de la formación de los productos finales de la glucosilación avanzada y su relación con las complicaciones de la diabetes mellitus. DESARROLLO: los productos finales de la glucosilación avanzada se producen por la reacción no enzimática de la glucosa y otros derivados glucados (glioxal, metilglioxal y 3-desoxiglucosona con grupos amino de proteínas de larga vida. La glucosilación altera la estructura, las propiedades físico-químicas y la función de las proteínas intracelulares y extracelulares. En la membrana basal de los pequeños vasos se produce un engrosamiento y una distorsión de su estructura, que ocasiona pérdida de la elasticidad de la pared vascular y una permeabilidad anormal de esta a las proteínas (disfunción endotelial, así como aumento de la génesis de especies reactivas del oxígeno. La unión de productos finales de la glucosilación avanzada a sus receptores de membrana favorece la producción citoquinas y factores de crecimiento por los macrófagos y células mesangiales. Todo lo anterior favorece al desarrollo de aterosclerosis. CONCLUSIONES: los productos finales de la glucosilación avanzada desempeñan un importante papel en el desarrollo de las complicaciones microvasculares y macrovasculares en el diabético. El control metabólico estricto de la glucemia y, en la actualidad, la terapéutica farmacológica con agentes que inhiben la formación de productos finales de la glucosilación avanzada o tienen acción antioxidante, constituyen alternativas terapéuticas para la prevención y solución del problema de las complicaciones crónicas de la

  1. Ambient pressure sensitivity of microbubbles investigated through a parameter study

    DEFF Research Database (Denmark)

    Andersen, Klaus Scheldrup; Jensen, Jørgen Arendt

    2009-01-01

    Measurements on microbubbles clearly indicate a relation between the ambient pressure and the acoustic behavior of the bubble. The purpose of this study was to optimize the sensitivity of ambient pressure measurements, using the subharmonic component, through microbubble response simulations....... The behavior of two microbubbles corresponding to two different contrast agents was investigated as a function of driving pulse and ambient overpressure, pov. Simulations of Levovist using a rectangular driving pulse show an almost linear reduction in the subharmonic component as pov is increased. For a 20...... found, although the reduction is not completely linear as a function of the ambient pressure....

  2. Simulation of microbubble response to ambient pressure changes

    DEFF Research Database (Denmark)

    Andersen, Klaus Scheldrup; Jensen, Jørgen Arendt

    2008-01-01

    The theory on microbubbles clearly indicates a relation between the ambient pressure and the acoustic behavior of the bubble. The purpose of this study was to optimize the sensitivity of ambient pressure measurements, using the subharmonic component, through microbubble response simulations....... The behaviour of two different contrast agents was investigated as a function of driving pulse and ambient overpressure, pov. Simulations of Levovist using a rectangular driving pulse show an almost linear reduction in the subharmonic component as pov is increased. For a 20 cycles driving pulse, a reduction...... is not completely linear as a function of the ambient pressure....

  3. Crisis ambiental, desastre natural y la "sociedad desierto"

    OpenAIRE

    Reynol Díaz Coutiño

    2010-01-01

    La crisis ambiental y los "desastres naturales" se muestran como un conjunto que está definido por la sensibilidad de los factores de correlación. La crisis ambiental se entiende como los cambios "inesperados" delos procesos de aquellos factores que forman el ambiente local. La transición silenciosa de tales cambios altera, cuando va más allá de ciertos límites, el frágil balance del medio ambiente, hasta que emerge el desastre. En este sentido, el desastre nos es natural, pero sí los fenómen...

  4. Effect of the culture conditions on the production of an extracellular protease by thermophilic Bacillus sp and some properties of the enzymatic activity Efeito das condições de cultivo sobre a produção de proteases extracelulares pelo termofílico Bacillus sp e algumas propriedades da atividade enzimática

    Directory of Open Access Journals (Sweden)

    Camila Rocha da Silva

    2007-06-01

    protease, enquanto lactose e sacarose não foram muito efetivas. O aumento da concentração de maltose no meio de cultura até 1% melhorou o crescimento do organismo e a atividade da enzima. Em relação à concentração de proteínas do soro e da água de maceração de milho no meio de cultura, 0,1% e 0,2% respectivamente, foram as mais efetivas para a secreção da enzima pelo organismo. Estudos sobre a caracterização da protease revelaram que a temperatura ótima desta enzima foi 70ºC. Em relação a termoestabilidade da enzima, a protease manteve 80% de sua atividade original após 2 h de tratamento a 60ºC. A 70ºC, 70% de sua atividade original foi mantida após 15 min. O pH ótimo para atividade da enzima foi 8,5. Após a incubação da solução enzimática bruta a pH 6,0-10.0 por 2 h a temperatura ambiente, foi observado um decréscimo de em torno de 15% da sua atividade original a pH 8,5. A pH 10,0 o decréscimo na atividade foi de 24%. Na presença de 1.0 M e 5.0 M NaCl, 76% e 37% da atividade da protease foi mantida após 2 h de incubação a 45ºC respectivamente.

  5. COMPLEJIDAD AMBIENTAL: PROPUESTAS ÉTICAS EMERGENTES DEL PENSAMIENTO AMBIENTAL LATINOAMERICANO

    Directory of Open Access Journals (Sweden)

    Noguera de Echeverri Ana Patricia

    2008-07-01

    Full Text Available En este artículo, se recogen las propuestas ético ambientales más importantes de la Escuela de Pensamiento Ambiental Latinoamericano, que ha ido construyéndose desde hace 30 años. Se destacan autores de primera línea en el campo de la construcción de Pensamiento Ambiental filosófico, como el Filósofo y Pensador Colombiano, de trascendencia latinoamericana, Augusto Ángel Maya, quien plantea la posibilidad de construir una Filosofía Ambiental Ético estética, dado que la Filosofía Moderna ha sido Metafísica, aún en sus corrientes empiristas, positivistas y neopositivistas. Se destaca la obra de Enrique Leff, mexicano, que propone los conceptos de Complejidad y Saber Ambiental, como puntos de partida de una ética en la educación, la ciencia y la tecnología, dentro de unas relaciones sistémicas y holísticas. Igualmente la propuesta ético estética compleja de Julio Carrizoza, colombiano, se hace presente en este ensayo, como una propuesta política fundamental ante problemas colombianos como son la violencia y la exclusión. Las propuestas de Filósofos colombianos de gran importancia en el campo de la Filosofía Política y Moral, que han desembocado en la propuesta de una Ética Ambiental, Pública y Comunicativa, como es el caso de Guillermo Hoyos Vásquez y de otros pensadores tanto de Colombia como de otros países latinoamericanos, se presentan en este espacio, con el fin de recoger de manera crítica nuestras ideas acerca de la posibilidad de una ética ambiental, es decir una ética descentrada, sin sujeto y sin objeto, una ética sistémica, donde los valores se construyen en las relaciones y donde la intricada trama de la vida, que ha venido construyéndose desde hace más de 2.500 millones de años, y de la cual somos meros hilos, pasa de ser objeto a ser valor primero en su más genuino sentido.

  6. Photocatalysts: ambient temperature destruction of VOCs

    Energy Technology Data Exchange (ETDEWEB)

    Miller, R [IT Corp., Oak Ridge, TN (United States)

    1994-12-31

    Photocatalysis was a failure as a solar energy driven organic synthesis technique, but as this study indicates, it has undergone a renaissance as a promising treatment method for volatile organic compounds (VOCs) in air streams. Photocatalytic oxidation (PCO) relies upon the ability of certain semiconductors to be stimulated by UV radiation. UV light excites valence band electrons in the semiconductor catalyst to jump to a conductance band leaving holes in the valence band. The electrons and holes can react with compounds such as organic contaminants present in an air stream. Hallmarks of the technology include rapid destruction kinetics for many VOCs at ambient temperature and efficient use energy in the form of UV-A photons. Studies clearly indicate that PCO is competitive on capital cost and offers significant operating cost savings on selected applications. 6 refs., 3 tabs., 2 figs.

  7. Photocatalysts: ambient temperature destruction of VOCs

    International Nuclear Information System (INIS)

    Miller, R.

    1994-01-01

    Photocatalysis was a failure as a solar energy driven organic synthesis technique, but as this study indicates, it has undergone a renaissance as a promising treatment method for volatile organic compounds (VOCs) in air streams. Photocatalytic oxidation (PCO) relies upon the ability of certain semiconductors to be stimulated by UV radiation. UV light excites valence band electrons in the semiconductor catalyst to jump to a conductance band leaving holes in the valence band. The electrons and holes can react with compounds such as organic contaminants present in an air stream. Hallmarks of the technology include rapid destruction kinetics for many VOCs at ambient temperature and efficient use energy in the form of UV-A photons. Studies clearly indicate that PCO is competitive on capital cost and offers significant operating cost savings on selected applications. 6 refs., 3 tabs., 2 figs

  8. Meio Ambiente e Estado Constitucional Cooperativo

    OpenAIRE

    Campello, Lívia Gaigher

    2014-01-01

    Este estudo partiu da percepção de que há, na atualidade, uma perspectiva integrativa, fundamentada no princípio do desenvolvimento sustentável, que requer como seu pressuposto a realização de valores éticos e solidários. Assim, esta noção tem refletido nos parâmetros da busca pelo crescimento econômico, que deve ocorrer em consonância com a preservação dos direitos das presentes e futuras gerações. Nesse sentido, buscou-se desvendar os fundamentos e finalidades do Direito Ambiental no âmbito...

  9. EL GIRO AMBIENTAL DE LAS CIENCIAS SOCIALES

    Directory of Open Access Journals (Sweden)

    Sara Victoria Alvarado

    2014-01-01

    Full Text Available Se pretenden problematizar aquí las ciencias sociales desde el efecto que la metáfora del sur ha generado en nuestra comprensión de lo social y desde el reconocimiento de las reflexiones que buscan articularse con la condición am - biental de la cultura. En un primer momento se retoma el dibujo América invertida de Joaquín Torres para insistir en el despliegue metafórico del sur, y en una segunda parte se repasa la noción de contrato natural para resignificar las prácticas discursivas de las ciencias sociales. Se concluye con la urgencia del giro ambiental como un nuevo escenario para este campo de investigación.

  10. Ambient Assisted Living : Italian forum 2013

    CERN Document Server

    Siciliano, Pietro; Germani, Michele; Monteriù, Andrea

    2014-01-01

    This book presents the refereed proceedings of the Fourth Italian Forum on Ambient Assisted Living (AAL), held in Ancona, Italy, in October 2013. A wide range of issues are covered, and new technological developments are described which will support the autonomy and independence of individuals with special needs through an innovative and integrated approach, designed to respond to the socio-economic challenges of an aging population. Topics addressed include: health and well-being, prevention and rehabilitation, and support for care providers; active aging and its social implications; services for the frail elderly with health problems and their families; nutrition; ICT platforms/technologies for the benefit of the elderly; home automation and control technologies (autonomy, safety, and energy saving); smart cities and smart communities; telemedicine, telerehabilitation, and telecare; mobility, participation, and social inclusion; games and fun for the elderly; building design; social housing; interface desig...

  11. Can Ambient Scent Enhance the Nightlife Experience?

    Science.gov (United States)

    Schifferstein, Hendrik N J; Talke, Katrin S S; Oudshoorn, Dirk-Jan

    2011-06-01

    Ever since smoking was prohibited in restaurants, bars, and clubs, undesirable smells that were previously masked by cigarette smoke became noticeable. This opens up opportunities to improve the dance club environment by introducing pleasant ambient scents that mask the unwanted odors and to allow competing clubs to differentiate themselves. A field study was conducted at three dance clubs using a 3 × 3 Latin square design with pre- and post-measurements of no-scent control conditions. The three scents tested were orange, seawater, and peppermint. These scents were shown to enhance dancing activity and to improve the evaluation of the evening, the evaluation of the music, and the mood of the visitors over no added scent. However, no significant differences were found between the three scents.

  12. Ambient air quality trends in Alberta

    International Nuclear Information System (INIS)

    2007-01-01

    This document provided an overview of ambient air pollutant trends in Alberta. The report discussed the following pollutants having effect on human and environmental health: carbon monoxide (CO), hydrogen sulphide (H2 S ), nitrogen dioxide (NO 2 ), sulphur dioxide (SO 2 ), ozone (O 3 ), fine particulate matter (PM 2 .5), benzene, and benzopyrene. Each of these pollutants was described. The report provided data on annual average concentration trends and annual 99th percentile concentration as an indicator of peak concentrations. A map illustrating air quality monitoring stations in 2006 was also provided. The findings revealed that mean annual CO levels were the lowest they have been since 1990; hydrogen sulphide concentrations have fluctuated in time since 1990; most Edmonton and Calgary area stations showed significant decreasing trends in annual average NO 2 levels since 1990; and higher SO 2 concentrations have been found in the industrial areas of Alberta, such as the Redwater and Scotford oil sands locations. tabs., figs

  13. Ambient and Unobtrusive Cardiorespiratory Monitoring Techniques.

    Science.gov (United States)

    Bruser, Christoph; Antink, Christoph Hoog; Wartzek, Tobias; Walter, Marian; Leonhardt, Steffen

    2015-01-01

    Monitoring vital signs through unobtrusive means is a goal which has attracted a lot of attention in the past decade. This review provides a systematic and comprehensive review over the current state of the field of ambient and unobtrusive cardiorespiratory monitoring. To this end, nine different sensing modalities which have been in the focus of current research activities are covered: capacitive electrocardiography, seismo- and ballistocardiography, reflective photoplethysmography (PPG) and PPG imaging, thermography, methods relying on laser or radar for distance-based measurements, video motion analysis, as well as methods using high-frequency electromagnetic fields. Current trends in these subfields are reviewed. Moreover, we systematically analyze similarities and differences between these methods with respect to the physiological and physical effects they sense as well as the resulting implications. Finally, future research trends for the field as a whole are identified.

  14. Wireless Sensor Networks for Ambient Assisted Living

    Directory of Open Access Journals (Sweden)

    Raúl Aquino-Santos

    2013-11-01

    Full Text Available This paper introduces wireless sensor networks for Ambient Assisted Living as a proof of concept. Our workgroup has developed an arrhythmia detection algorithm that we evaluate in a closed space using a wireless sensor network to relay the information collected to where the information can be registered, monitored and analyzed to support medical decisions by healthcare providers. The prototype we developed is then evaluated using the TelosB platform. The proposed architecture considers very specific restrictions regarding the use of wireless sensor networks in clinical situations. The seamless integration of the system architecture enables both mobile node and network configuration, thus providing the versatile and robust characteristics necessary for real-time applications in medical situations. Likewise, this system architecture efficiently permits the different components of our proposed platform to interact efficiently within the parameters of this study.

  15. Spatial audio reproduction with primary ambient extraction

    CERN Document Server

    He, JianJun

    2017-01-01

    This book first introduces the background of spatial audio reproduction, with different types of audio content and for different types of playback systems. A literature study on the classical and emerging Primary Ambient Extraction (PAE) techniques is presented. The emerging techniques aim to improve the extraction performance and also enhance the robustness of PAE approaches in dealing with more complex signals encountered in practice. The in-depth theoretical study helps readers to understand the rationales behind these approaches. Extensive objective and subjective experiments validate the feasibility of applying PAE in spatial audio reproduction systems. These experimental results, together with some representative audio examples and MATLAB codes of the key algorithms, illustrate clearly the differences among various approaches and also help readers gain insights on selecting different approaches for different applications.

  16. True Visions The Emergence of Ambient Intelligence

    CERN Document Server

    Aarts, Emile

    2006-01-01

    Ambient intelligence (AI) refers to a developing technology that will increasingly make our everyday environment sensitive and responsive to our presence. The AI vision requires technology invisibly embedded in our everyday surroundings, present whenever we need it that will lead to the seamless integration of lighting, sounds, vision, domestic appliances, and personal healthcare products to enhance our living experience. Written for the non-specialist seeking an authoritative but accessible overview of this interdisciplinary field, True Visions explains how the devices making up the AI world will operate collectively using information and intelligence hidden in the wireless network connecting them. Expert contributions address key AI components such as smart materials and textiles, system architecture, mobile computing, broadband communication, and underlying issues of human-environment interactions. It seeks to unify the perspectives of scientists from diverse backgrounds ranging from the physics of materia...

  17. Bacterial decontamination using ambient pressure nonthermal discharges

    Energy Technology Data Exchange (ETDEWEB)

    Birmingham, J.G.; Hammerstrom, D.J.

    2000-02-01

    Atmospheric pressure nonthermal plasmas can efficiently deactivate bacteria in gases, liquids, and on surfaces, as well as can decompose hazardous chemicals. This paper focuses on the changes to bacterial spores and toxic biochemical compounds, such as mycotoxins, after their treatment in ambient pressure discharges. The ability of nonthermal plasmas to decompose toxic chemicals and deactivate hazardous biological materials has been applied to sterilizing medical instruments, ozonating water, and purifying air. In addition, the fast lysis of bacterial spores and other cells has led us to include plasma devices within pathogen detection instruments, where nucleic acids must be accessed. Decontaminating chemical and biological warfare materials from large, high value targets such as building surfaces, after a terrorist attack, are especially challenging. A large area plasma decontamination technology is described.

  18. UNCED : La Cumbre Ambiental de Brasil /92

    OpenAIRE

    1992-01-01

    El mundo está a las puertas de celebrar una de las más importantes cumbres políticas, en la cual podrían tomarse decisiones de suma trascendencia que repercutirán en forma directa en la dinámica futura de la vida en el planeta. UNCED-92 (Conferencia de las Naciones Unidas sobre Medio Ambiente y Desarrollo que se realizará en Río de Janeiro), es la convocatoria a las más altas esferas de decisión política de los países miembros para concertar acuerdos que definirán, en buena parte, el perfil d...

  19. Assessment of SRS ambient air monitoring network

    Energy Technology Data Exchange (ETDEWEB)

    Abbott, K. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL); Jannik, T. [Savannah River Site (SRS), Aiken, SC (United States). Savannah River National Lab. (SRNL)

    2016-08-03

    Three methodologies have been used to assess the effectiveness of the existing ambient air monitoring system in place at the Savannah River Site in Aiken, SC. Effectiveness was measured using two metrics that have been utilized in previous quantification of air-monitoring network performance; frequency of detection (a measurement of how frequently a minimum number of samplers within the network detect an event), and network intensity (a measurement of how consistent each sampler within the network is at detecting events). In addition to determining the effectiveness of the current system, the objective of performing this assessment was to determine what, if any, changes could make the system more effective. Methodologies included 1) the Waite method of determining sampler distribution, 2) the CAP88- PC annual dose model, and 3) a puff/plume transport model used to predict air concentrations at sampler locations. Data collected from air samplers at SRS in 2015 compared with predicted data resulting from the methodologies determined that the frequency of detection for the current system is 79.2% with sampler efficiencies ranging from 5% to 45%, and a mean network intensity of 21.5%. One of the air monitoring stations had an efficiency of less than 10%, and detected releases during just one sampling period of the entire year, adding little to the overall network intensity. By moving or removing this sampler, the mean network intensity increased to about 23%. Further work in increasing the network intensity and simulating accident scenarios to further test the ambient air system at SRS is planned

  20. Ambientes virtuales de aprendizaje utilizando realidad aumentada

    Directory of Open Access Journals (Sweden)

    Diana Nancy Martínez García

    2018-03-01

    Full Text Available Realidad aumentada o Augmented reality es una nueva tecnología que al combinarla con espacios físicos permite ampliar el espectro de la información relacionada con dichos espacios; esto se hace a través de la superposición de imágenes, información o material audiovisual específico aplicado a cualquier área de conocimiento. Los ambientes virtuales de aprendizaje basados en realidad aumentada prometen innovación debido a que transforman los escenarios de aprendizaje real mediante el uso de nuevas tecnologías aplicadas al ámbito educativo, complementando de esta manera la percepción e interacción con el mundo real; logrando el acceso a nuevos contenidos prescindiendo de recursos físicos. En cuanto al uso de la realidad aumentada aplicada al área de enfermería, existe un sinnúmero de herramientas para complementar una clase magistral y enriquecer el proceso de aprendizaje. Sin embargo, la mayoría de los desarrollos realizados utilizando realidad aumentada proviene de contextos diferentes al nuestro, por lo que resulta importante fomentar el uso de este tipo de herramientas para que suscite el interés de los desarrolladores locales de tecnologías aplicadas al ámbito educativo y más específicamente el de salud. El objetivo de la presente revisión fue fundamentar teóricamente acerca de los ambientes virtuales de aprendizaje utilizando aplicaciones basadas en realidad aumentada.

  1. Near-ambient solid polymer fuel cell

    Science.gov (United States)

    Holleck, G. L.

    1993-01-01

    Fuel cells are extremely attractive for extraterrestrial and terrestrial applications because of their high energy conversion efficiency without noise or environmental pollution. Among the various fuel cell systems the advanced polymer electrolyte membrane fuel cells based on sulfonated fluoropolymers (e.g., Nafion) are particularly attractive because they are fairly rugged, solid state, quite conductive, of good chemical and thermal stability and show good oxygen reduction kinetics due to the low specific adsorption of the electrolyte on the platinum catalyst. The objective of this program is to develop a solid polymer fuel cell which can efficiently operate at near ambient temperatures without ancillary components for humidification and/or pressurization of the fuel or oxidant gases. During the Phase 1 effort we fabricated novel integral electrode-membrane structures where the dispersed platinum catalyst is precipitated within the Nafion ionomer. This resulted in electrode-membrane units without interfacial barriers permitting unhindered water diffusion from cathode to anode. The integral electrode-membrane structures were tested as fuel cells operating on H2 and O2 or air at 1 to 2 atm and 10 to 50 C without gas humidification. We demonstrated that cells with completely dry membranes could be self started at room temperature and subsequently operated on dry gas for extended time. Typical room temperature low pressure operation with unoptimized electrodes yielded 100 mA/cm(exp 2) at 0.5V and maximum currents over 300 mA/cm(exp 2) with low platinum loadings. Our results clearly demonstrate that operation of proton exchange membrane fuel cells at ambient conditions is feasible. Optimization of the electrode-membrane structure is necessary to assess the full performance potential but we expect significant gains in weight and volume power density for the system. The reduced complexity will make fuel cells also attractive for smaller and portable power supplies and as

  2. Ambient noise tomography of Lo'ihi

    Science.gov (United States)

    McClement, K.; Thurber, C. H.; Teel, A.; Caplan-Auerbach, J.

    2012-12-01

    Lo'ihi seamount, the youngest volcano in the Hawaiian-Emperor chain, lies approximately 30 km south of Hawai'i Island with its summit still approximately 1 km below sea level. Lo'ihi offers a unique opportunity to study the early formation of a hotspot volcano and can provide insight into the deep internal structure of the other volcanoes that make up the Hawaiian Islands. This study uses Ambient Noise Tomography (ANT) to create a 3D tomographic image of Lo'ihi's S-wave velocity structure from ocean bottom seismometer (OBS) data. ANT has been used in many subaerial studies but has seen very few applications to OBS data. This study uses continuous data recorded in 2010 to 2011 from 12 short-period OBS instruments deployed on and around Lo'ihi. With the farthest distance between stations being just over 30 km, the stations provide a fairly dense coverage mainly for the northern half of the volcano. Following the approach of Masterlark et al. [2010], we computed vertical-vertical and vertical-radial cross-correlations using 97 days of continuous data from the 12 stations to produce the ambient noise Green's functions. From these, dispersion curves were produced over a frequency range from .04 Hz to 0.65 Hz . After a quality control analysis, checkerboard tests were used to determine a suitable cell size for the 2D group velocity inversions. The final step is the inversion of the group velocity dispersion curves to create a 3D Vs model. The 3D Vs image produced through this method does not provide clear evidence of a shallow magma chamber; however, when compared to a previous P-wave velocity (Vp) model [Caplan-Auerbach, 2001], a high Vp/Vs ratio is evident especially at depths from 1 km to 5 km, indicating the presence of highly fractured rock.

  3. DESIGUALDADE AMBIENTAL, ECONOMIA E POLÍTICA

    Directory of Open Access Journals (Sweden)

    Henri Acselrad

    2013-12-01

    Full Text Available Otexto discute as razões pelas quais a adoção efetiva de mudançasnas práticas e padrões técnico-espaciais do capitalismo destinadasa ajustar o ritmo da acumulação intensiva e as fronteiras daacumulação extensiva aos requisitos da reprodução utilitária deecossistemas não ocorreu até aqui – para além do que a própriadinâmica mercantil o tenha admitido. Sustenta que, por vigorar defato uma divisão socio-espacial da degradação ambiental, os danosambientais da acumulação são, de forma sistemática, destinadosaos grupos sociais e étnicos dominados – seja pela expropriaçãodas bases territoriais de formas sócio-produtivas não hegemônicas,seja pela deterioração das bases reprodutivas de grupos sociais quenão se integram ao circuito do capital a não ser como consumidoresforçados dos produtos invendáveis da atividade capitalista – achamada poluição. A tecnologia social acionada para a imposiçãodesigual de riscos é, no plano intra-nacional ou internacional, nocapitalismo liberalizado, a chantagem locacional dos investimentos –ou seja, a ameaça de deslocalização dos empreendimentos com acolocação dos trabalhadores em situação de competição, não sóno que diz respeito ao nível de seus salários, mas também aosdireitos e condições normativas destinadas a assegurar proteçãosocial e ambiental.

  4. Derecho ambiental municipal: las intendencias frente a la problemática del medio ambiente

    Directory of Open Access Journals (Sweden)

    Gastón Casaux

    2014-11-01

    Full Text Available Trabajo presentado en las III Jornadas de Derecho Municipal celebradas del 9 al 11 de setiembre de 1998.en el Palacio Munieipal, con el auspicio de la IMM y la Éditorial Nueva Juridica.Contenido: Panorama de las competencias municipales a través de la ley 2.820 de 1903. Ley Orgánica Municipal 9515 de 1935 y capítulos de la Constitución de 1966 (arts.7,72 y 332. La Reforma Constitucional de 8 de diciembre de 1996 aprobada por la Asamblea General el 14 de enero de 1997, constitucionaliza el Derecho Ambiental y el Congreso Nacional de Intendentes. Naturaleza Jurídica, del Plenario Municipal Nacional. Antecedentes, vía de hecho, distintas materias, unidad y organicidad. El advenimiento del Derecho Ambiental en la legislación contemporánea y su incorporación en el ámbito municipal. El siglo XXI, el Derecho Ambiental, el rol de los Municipios y su trascendencia para el ciudadano

  5. Produção de complexos enzimáticos por A. niger a partir de soja por fermentação em estado sólido - doi: 10.4025/actascitechnol.v34i2.9447

    Directory of Open Access Journals (Sweden)

    Leandro Daniel De Paris

    2012-03-01

    Full Text Available A soja possui ampla disponibilidade de componentes bioquímicos como proteínas, polissacarídeos, lipídios, carboidratos, minerais, vitaminas, fibras, lecitina, dentre outros, tornando interessante sua utilização como substrato para a fermentação em estado sólido (FES. Este processo, apesar de não ser tão utilizado industrialmente quanto à fermentação submersa (Fsm, apresenta-se viável, pois tem apresentado resultados superiores de produtividade, principalmente no cultivo de fungos filamentosos e na produção de enzimas. Assim, o objetivo deste trabalho foi o de comparar a produção de diferentes tipos de complexos enzimáticos por FES utilizando diferentes tipos de sojas (orgânica, transgênica e convencional com o fungo Aspergillus niger. Realizou-se a caracterização dos substratos para a produção de amilase, protease e lipase, avaliação das curvas de crescimento do microrganismo e das condições ótimas de produção da protease utilizando planejamento de experimentos. As maiores atividades enzimáticas no estudo dos parâmetros da fermentação foram observadas para a protease, utilizando soja convencional com umidade inicial de 50%, 144h de fermentação, concentração inicial do inóculo de 4.106 esporos g-1 e tamanho de partícula de 0,6 mm. O estudo de otimização do processo indicou que os melhores resultados de atividade enzimática para a protease foram obtidos em pH 3,0 e tamanho de partícula dos grãos de soja de 0,6 mm após a fragmentação para as fermentações.

  6. Freqüência e atividade enzimática de Candida albicans isoladas da mucosa bucal de crianças de uma creche da prefeitura de Fortaleza Frequency and enzymatic activity of Candida albicans isolated from the buccal mucosa of children of a day-care center of the city hall of Fortaleza, Ceará, Brazil

    Directory of Open Access Journals (Sweden)

    Everardo Albuquerque Menezes

    2005-02-01

    Full Text Available As candidíases bucais (também chamadas sapinhos que ocorrem em crianças são causadas por uma deficiência imunológica, bem como por outros fatores tais como má higiene bucal e esterilização inadequada dos utensílios utilizados pelas mesmas, que potencializam a ocorrência dessa infecção fúngica. Considerando esse fato, foram avaliadas a freqüência e a atividade enzimática de Candida sp. isoladas em crianças de uma creche pública (Aprisco na cidade de Fortaleza, Ceará. Foram coletadas amostras da mucosa bucal de 364 alunos de 1 a 5 anos de idade. Elas foram semeadas em ágar Sabouraud dextrose com cloranfenicol, incubadas por 72 horas a 37ºC e identificadas por testes micológicos. Verificou-se que 67 (18% apresentaram leveduras do gênero Candida. A Candida albicans foi a mais freqüente, com 30 isolados (45%, seguida pelas C. tropicalis (31%, C. guilliermondii (17%, C. glabrata (4,5% e C. stellatoidea (1,5%. Com relação às atividades enzimáticas das cepas de Candida albicans, 20% produziram a enzima proteinase e 33%, a fosfolipase. As Candida albicans isoladas da mucosa bucal de crianças dessa creche da prefeitura apresentaram uma fraca atividade enzimática. Assim, conclui-se que essas cepas parecem ter uma baixa virulência.Immunedefficiency is one of the main causes of buccal candidiasis, also called thrush, in children. Other factors like inadequate mouth hygiene and inappropriate sterilization utensils potentialize this fungal infection. Considering these facts, Candida sp. frequency and enzymatic activity were evaluated in 364 stocks from mouth mucous of one to five year-old children from a public day care center in Fortaleza, Ceará (Brazil. The samples were cultured in dextrose Sabouraud with chloranfenicol agar and incubated for 72 hours at 37°C. They were identified by mycological tests. It was verified that 67 samples (18% presented Candida sp. and the most frequent genus was Candida albicans (30

  7. Degradabilidade in situ de silagens de milho confeccionadas com inoculantes bacteriano e/ou enzimático - DOI: 10.4025/actascianimsci.v28i1.658 In situ degradability of corn silages prepared with bacterial and/or enzymatic inoculants - DOI: 10.4025/actascianimsci.v28i1.658

    Directory of Open Access Journals (Sweden)

    Elzânia Sales Pereira

    2006-01-01

    Full Text Available O objetivo deste trabalho foi avaliar os efeitos de inoculantes bacterianos e/ou enzimáticos sobre a degradabilidade ruminal da silagem de milho. Foi utilizada a técnica in situ, em quatro bovinos adultos, distribuídos em quadrado latino 4x4. Os tratamentos avaliados foram: SC (silagem controle, SIB (silagem com inoculante bacteriano, SIBE (silagem com inoculante bacteriano e enzimático e SIE (silagem com inoculante enzimático. Não houve diferença entre tratamentos nas frações solúvel (a, potencialmente degradável (b, taxa de degradação da fração b (c, degradabilidade potencial (DP e degradabilidade efetiva (DE da MS e MO. A DE da PB foi maior para o tratamento SIE (63,13% e menor para o tratamento SIBE (53,69%. A fração b da FDN apresentou maior valor para SIBE (74,13% e menor para SIB (64,07%. O resíduo indigerido (I da FDN não diferiu entre os tratamentos. As frações b e I e a taxa c da FDA não diferiram entre os tratamentos. Palavras-chave: degradação, fibra, matéria orgânica, matéria seca, proteína bruta.The objective of this work was to evaluate the effects of the bacterial and/or enzymatic inoculants on corn silage degradation. The in situ technique was used in four adult steers in a 4x4 latin square design. The evaluated treatments were: CS (control silage, SBI (silage with bacterial inoculant, SBEI (silage with bacterial and enzymatic inoculant and SEI (silage with enzymatic inoculant. There was no difference among treatments in soluble fraction (a, potential degradable fraction (b, fraction b rate of degradation (c, potential degradability (PD and effective degradability (ED of DM and OM. The ED of CP was higher in SEI treatment (63.13% and lower in SBEI treatment (53.69%. The b fraction of NDF was higher for SBEI (74.13% and lower for SBI (64.07%. The NDF indigestible residue (I did not show any difference among treatments. The ADF b and I fraction and the c rate values did not show any difference among

  8. Exploring distributed user interfaces in ambient intelligent environments

    NARCIS (Netherlands)

    Dadlani Mahtani, P.M.; Peregrin Emparanza, J.; Markopoulos, P.; Gallud, J.A.; Tesoriero, R.; Penichet, V.M.R.

    2011-01-01

    In this paper we explore the use of Distributed User Interfaces (DUIs) in the field of Ambient Intelligence (AmI). We first introduce the emerging area of AmI, followed by describing three case studies where user interfaces or ambient displays are distributed and blending in the user’s environments.

  9. Designing interactive ambient multimedia applications : requirements and implementation challenges

    NARCIS (Netherlands)

    Obrenovic, Z.; Nack, F.-M.; Hardman, H.L.

    2006-01-01

    Ambient intelligence opens new possibilities for interactive multimedia, leading towards applications where the selection, generation and playback of multimedia content can be directed and influenced by multiple users in an ambient sensor network. In this paper, we derive the basic requirements for

  10. Ambient Response Analysis of the Great Belt Bridge

    DEFF Research Database (Denmark)

    Brincker, Rune; Frandsen, Jeanette B.; Andersen, Palle

    2000-01-01

    In this paper an ambient response analysis of the Great Belt Bridge is presented. The Great Belt Bridge is one of the largest suspension bridges in the world, and the analysis was carried out in order to investigate the possibilities of estimating reliable damping values from the ambient response...

  11. 75 FR 81126 - Revisions to Lead Ambient Air Monitoring Requirements

    Science.gov (United States)

    2010-12-27

    ... tons per year of lead is necessary to provide sufficient information about airborne lead levels near... Revisions to Lead Ambient Air Monitoring Requirements AGENCY: Environmental Protection Agency (EPA). ACTION...) that revised the primary and secondary National Ambient Air Quality Standards (NAAQS) for lead and...

  12. Dioxins and environment Dioxinas y medio ambiente

    Directory of Open Access Journals (Sweden)

    Jimena García Lobo

    2011-12-01

    Full Text Available With the generic term of dioxin is named the groups of the polychlorinated dibenzo-p-dioxins (PCDD and the polychlorinated dibenzofuran (PCDF, typical representans of the persistent organic compounds (POPs. They are obtained as not desired secondary products from several industrial process in which chlorine is used in some of its phases. The dioxins have centrated in the last decade an important part if the medical investigations in environmental health do to its notable toxicity, since they are one of the more powerful toxic chemical substances created by mankind, characterized for affecting the nervous and inmunitary system, being implicated into appearance of difference types of cancer and causing the appearance of several endocrine disorders, because of what they have been nowadays classified as endocrine disruptors. On degradation, bioaccumulation and on a large scale atmospheric transport capacity between the several environmental phases, make them to be considerate nowadays as dangerous compounds for human beings.Con el término genérico dioxinas se designa al grupo de las dibenzo-p-dioxinas policloradas (PCDD y de los dibenzofuranos policlorados (PCDF, representantes típicos de los compuestos orgánicos persistentes (COPs. Se obtienen como productos secundarios no deseados de diversos procesos industriales en los que se emplea cloro en alguna de sus etapas. Las dioxinas han centrado en la última década una parte importante de la investigación médica en salud ambiental debido a su notable toxicidad, ya que son las sustancias químicas peligrosas más potentes creadas por el hombre, afectando al sistema nervioso e inmunitario, estando implicadas en la aparición de distintos tipos de cáncer y provocando la aparición de alteraciones hormonales, clasificándose actualmente como disruptores endocrinos. Por otra parte, su persistencia en el medio ambiente, resistencia a la degradación, bioacumulación y capacidad de transporte atmosf

  13. Risk related behaviour under different ambient scent conditions

    Directory of Open Access Journals (Sweden)

    Alina Gagarina

    2016-09-01

    Full Text Available The article analyses the effect of two ambient scents (peppermint and vanilla and their intensiveness on risk related behaviour that is expressed through selected decision making heuristics. Purpose of the article: The purpose of this article is to identify the relationship of ambient scent type and intensiveness with risk related behaviour that is expressed through selected decision making heuristics. Methodology/methods: 2×2 factorial experiment with control group was run. Ambient scent type (vanilla vs. peppermint and intensiveness (8 (1mg vs. 16 sprays (2mg of scent concentrate in the same room were manipulated as between subject variables. Risk aversion, effect of anchoring heuristic on bidding, and affect (risk and benefit heuristics were tracked as dependent variables. Scientific aim: To identify whether ambient scent type and intensiveness have effect on risk related behaviour. Findings: Evidence suggests that there are effects of ambient scent on risk related behaviour, thus fulfilling the missing gap to relate ambient environment to decision making heuristics when risks are involved. However, not all heuristics were affected by experimental conditions. Subjects were bidding significantly higher amounts under low anchor conditions, when peppermint scent was around (if compared to vanilla group. Affect risk was perceived as lower in peppermint ambient scent conditions, if compared to the control group. Intensity of ambient scent also had influence on affect risk: subjects perceived less risk under high scent intensity conditions. Conclusions: By manipulating ambient scent, marketers may reduce or increase consumers risk perception and behaviour and as a consequence influence their purchase decisions. Marketers could use peppermint scent in high intensiveness in the situations where they want consumers to undertake higher risks (expensive purchases, gambling, insurance, since stakes were higher under peppermint ambient scent condition

  14. Pre and Postharvest Enzymatic Activity in Gulupa (Passiflora edulis Sims Fruits from the Colombian Lower Montane Rain Forest / Actividad Enzimática Precosecha y Poscosecha en Frutos de Gulupa (Passiflora edulis Sims, en Condiciones del Bosque Húmedo Mo

    Directory of Open Access Journals (Sweden)

    Germán Franco

    2014-03-01

    Full Text Available “High-Andean fruits” are deemed important because oftheir potential for domestic consumption and exportation. Among them, gulupa (Passiflora edulis Sims is an exotic fruit of good acceptance in European markets. However, the technological support associated with the crop is incipient and its short shelf life leads to rapid deterioration of the product. This fact makes it necessary to investigate the physical, physiological and biochemical processes that characterize fruit ripening, in order to take actions to ensure that it arrives in its best possible condition to the consumer. In this context, the current study aimed at identifying enzymatic activity in gulupa fruits during pre and postharvest. Plant materialfrom the Colombian Gene Bank (administered by Corpoica was used. Fruits of known age were periodically harvested to determine the activity of the enzymes α-amylase, polygalacturonase (PG, pectinmethylesterase (PME and polyphenol oxidase (PPO through destructive samplings. It was found that α-amylase and PG are linked to the increase of soluble solids, which favors the sweet taste of the fruit. In turn, the low activity of PPO enables agroindustrial processing without severe fruit browning. / Los “frutales alto-andinos”, se consideran importantes por su potencial de consumo nacional y exportación. Entre ellos está la gulupa (Passiflora edulis Sims, reconocida como un frutal exótico de buena aceptación en mercados europeos. Sin embargo, el respaldo tecnológico asociado al cultivo, es incipiente y su corta vida poscosecha conduce al rápido deterioro del producto. Esto hace necesario plantear estudios de los procesos físicos, fisiológicos y bioquímicos que caracterizan la maduración, con el fin de procurar que el fruto llegue en las mejores condiciones de calidad a los consumidores. El estudio tuvo como objetivo conocer la actividad enzimática en los frutos de gulupa en precosecha y en poscosecha, con el

  15. Efeito do tratamento térmico e enzimático nas propriedades de filmes de gelatina Effect of thermal and enzymatic treatment on the properties of gelatin films

    Directory of Open Access Journals (Sweden)

    Rosemary Aparecida de Carvalho

    2006-09-01

    Full Text Available Dentre os fatores que afetam a atividade da enzima transglutaminase, a temperatura de reação ou incubação é um fator determinante no grau de reticulação. Por outro lado, para a gelatina, tipicamente a rede estrutural polimérica é estabilizada por forças secundárias, sendo que a formação da matriz polimérica envolve um delicado balanço entre interações polímero-polímero e polímero-solvente, e este balanço é fortemente dependente do histórico térmico da solução. Desta forma, o objetivo deste trabalho foi avaliar o efeito da temperatura na reação de modificação enzimática em relação às propriedades funcionais dos filmes modificados à base de gelatina (propriedades mecânicas, de barreira ao vapor de água, solubilidade em água e parâmetros de cor dos filmes. Viscosidade aparente das soluções filmogênicas foram também avaliadas. Foram produzidos filmes denominados nativo (FN, modificado enzimaticamente (FME e termicamente tratado (FC. De acordo com os resultados obtidos, observou-se que a temperatura de reação não afetou as propriedades mecânicas e a solubilidade dos diferentes filmes estudados. Por outro lado, filmes modificados enzimaticamente (FME na temperatura de 50 °C apresentaram permeabilidade ao vapor de água significantemente inferior aos produzidos nas demais temperaturas e tratamentos (FN e FC. O tratamento térmico também provocou redução da permeabilidade ao vapor de água.The activity of transglutaminase (one crosslinking enzyme can be affected by the temperature of reaction. Gelatin matrix gel formation depends on the balance between polymer-polymer interactions and a polymer-solvent solution. This balance also depends on the amount and type of thermal treatment to the gelatin solution. The aim of this work is to evaluate the effect of the temperature on the crosslinking reaction using transglutaminase. Mechanical properties, water vapor permeability, water solubility and color

  16. Influência das variáveis de processo na alcoólise enzimática de óleo de mamona Evaluation of the influence of process variables on enzimatic alcoholysis of castor oil

    Directory of Open Access Journals (Sweden)

    D. Oliveira

    2004-06-01

    Full Text Available O potencial de aplicação de lipases em processos biotecnológicos para a modificação de óleos e gorduras tem sido objeto de grande interesse nos meios científico, econômico e industrial nos últimos anos. Além da atividade de hidrólise de ésteres, as lipases podem catalisar uma grande variedade de reações de esterificação, transesterificação e poliesterificação. A transesterificação inclui acidólise, interesterificação e alcoólise. Neste trabalho reações de alcoólise de óleo de mamona para produção de ésteres de ácidos graxos foram estudadas devido a sua importância na obtenção de, por exemplo, agentes de antifricção, emulsificantes, intermediários para produzir uma numerosa quantidade de oleoquímicos e combustível alternativo ao diesel e/ou aditivo ao diesel de petróleo (biodiesel. Neste contexto, foi estudada a etanólise enzimática de óleo de mamona com lipase comercial (Lipozyme IM usando n-hexano como solvente. Os experimentos foram realizados variando a temperatura, as concentrações de água e enzima no meio reacional e a razão molar óleo-etanol, de acordo com um planejamento de experimentos pré-estabelecidos. Um modelo empírico foi utilizado para avaliar a influência das variáveis de processo no rendimento e, desta forma, as condições de operação que maximizam a produção de ésteres foram estabelecidas para a enzima utilizada.The use of lipases as biocatalysts in ester synthesis has been the object of growing interest. In addition to glycerol ester hydrolysis, lipases can catalyze a wide variety of esterification, transesterification and polyesterification reactions. The transesterification includes acidolysis, interesterification and alcoholysis. We consider in this report the application of lipases in the alcoholysis of brazilian vegetable oil in the production of fatty acid esters due to its importance as, for example, antifriction agents, food preservatives, emulsifiers and

  17. Ambient noise levels in the Taiwan region

    Science.gov (United States)

    Liang, W.; Liu, C.; Chen, R.; Huang, B.; Wu, F. T.; Wang, C.

    2008-12-01

    To characterize the island-wide background seismic noise in Taiwan, we estimate the power spectral density (PSD) at broadband stations of both the BATS (Broadband Array in Taiwan for Seismology) and the TAIGER experiment (Apr. 2006~Apr. 2008) for periods ranging from ~0.2 to 100 seconds. A new approach to calculate the probability density functions of noise power (PDFs, MaNamara and Buland, 2004) is used in this study. The results indicate that the cultural noise at higher frequencies is significant at populated area, which shows diurnal and weekly variation as what we expected. The noise power for microseisms centered at a period of ~5 seconds around the western costal plain show ~20dB higher than what observed at eastern Taiwan. This observation supports the inference that the coastal regions having narrow shelf with irregular coastlines are know to be especially efficient at radiating the predominat microseisms. Results from the linear array across central Taiwan demonstrate that the average noise power is quietest at the eastern Central Range. We have mapped the PDF mode for stations at various periods to see the spatial distribution of ambient noise levels, which could be used as the basic information for future station siting. Temporal variation of noise PSD is also present to provide a quantitative description of the seismic data quality collected by both BATS and TAIGER experiment. Some operational problems like base tilt, sensitivity change can be identified easily as well.

  18. 5th International Symposium on Ambient Intelligence

    CERN Document Server

    Novais, Paulo; Nihan, Céline; Rodríguez, Juan

    2014-01-01

    Ambient Intelligence (AmI) is a recent paradigm emerging from Artificial Intelligence (AI), where computers are used as proactive tools assisting people with their day-to-day activities, making everyone’s life more comfortable. Another main concern of AmI originates from the human computer interaction domain and focuses on offering ways to interact with systems in a more natural way by means user friendly interfaces. This field is evolving quickly as can be witnessed by the emerging natural language and gesture based types of interaction. The inclusion of computational power and communication technologies in everyday objects is growing and their embedding into our environments should be as invisible as possible. In order for AmI to be successful, human interaction with computing power and embedded systems in the surroundings should be smooth and happen without people actually noticing it. The only awareness people should have arises from AmI: more safety, comfort and wellbeing, emerging in a natural and inh...

  19. 8th International Symposium on Ambient Intelligence

    CERN Document Server

    Julián, Vicente; Villarrubia, Gabriel; Marreiros, Goreti; Novais, Paulo

    2017-01-01

    Ambient Intelligence (AmI) is a recent paradigm emerging from Artificial Intelligence, in which computers are used as proactive tools to assist people with their day-to-day activities, making their lives more comfortable. Another main goal of AmI originates from the human/computer interaction domain and focuses on offering ways to interact with systems in a more natural way by means of user-friendly interfaces. This field is evolving quickly, as can be witnessed by the emerging natural-language-based and gesture-based types of interaction. The inclusion of computational power and communication technologies in everyday objects is growing, and their embeddedness in our environments should be as invisible as possible. In order for AmI to be successful, human interaction with computing power and embedded systems in the surroundings should be smooth and take place without people actually noticing it. The only things people should notice in connection with AmI are more safety, comfort and wellbeing, emerging in a n...

  20. Banco del Sur y medio ambiente

    Directory of Open Access Journals (Sweden)

    Fabiano Crespilho

    2010-01-01

    Full Text Available La presente colaboración pone en la mesa del debate los objetivos de un banco de desarrollo, en la tradición económica-desarrollista, donde la satisfacción del desarrollo de los recursos naturales intentan mejorar no las condiciones de la población sino lograr las ventajas comparativas y el valor agregado que da la inserción al mercado mundial, frente a lo que debería ser un banco de desarrollo que invierta esa ecuación. La explotación de los recursos de una parte importante de la Amazonia está ligada a fomentar toda una serie de obras de infraestructura que harían un gran daño no sólo ecológico a la región sino a generaciones futuras. El autor discute la difícil problemática entre un banco de desarrollo que busca el equilibrio con el medio ambiente y un banco de desarrollo ligado a los intereses tanto del Estado como de las oligarquías exportadoras.

  1. ESPACIOS RENATURALIZADOS COMO AMBIENTES DE APRENDIZAJE

    Directory of Open Access Journals (Sweden)

    Yenny Rosas

    2015-12-01

    Los espacios renaturalizados, se consolidan como ambientes de aprendizaje para el ejercicio de la ciudadanía y la convivencia, a partir del desarrollo de metodologías participativas y haciendo uso de tecnologías apropiadas. Para ello se diseñaron e implementaron una serie de sesiones, con el fin de contribuir a la transformación del entorno y de permitir la conectividad ecológica presente en el ecosistema estratégico ubicado allí mismo, además de consolidar experiencias en el desarrollo de capacidades ciudadanas esenciales. Para la institución educativa se construyó, un jardín agroecológico y se sembraron árboles con fines productivos y ornamentales, permitiendo de esa manera la generación de identidad con respecto a los espacios renaturalizados, la estructura ecológica, además de reconocer las relaciones socio-ecológicas que alrededor de estos espacios se desarrollan. Entorno a la intervención, se construyeron conocimientos propios del currículo, además se identificaron las problemáticas ambientales propias de la zona, permitiendo de esa manera la armonización de la relación naturaleza-cultura y la adaptación ecosistémica al cambio climático, que requiere la ciudad de Bogotá.

  2. EVALUACION DEL IMPACTO AMBIENTAL EN OBRAS VIALES

    Directory of Open Access Journals (Sweden)

    Wilfredo Martínez D.

    2014-11-01

    Full Text Available El presente artículo es escrito, con el propósito de proporcionar elementos técnicos que contribuyan para orientar a los equipos de trabajo multidisciplinario, en valorar objetivamente los impactos ambientales provocados por la construcción de obras civiles y que suscitan el interés del dominio público y profesionales en la materia. Para ello hemos contado con la experiencia acumulada en instituciones gubernamentales y de índole privado y con nuestra experiencia profesional, en la construcción de obras civiles. El contenido del mismo, toma en cuenta varios criterios de especialistas en la materia y recursos disponibles; con ética y rigurosidad técnica. El objetivo de la evaluación de impactos ambientales, es predecir sobre la marcha y a futuro, estados alternativos de recursos y ambiente; convencidos que el cuido de la naturaleza puede convocar la voluntad de todas las partes y lograr trabajar armoniosamente, por la construcción de un futuro con desarrollo sostenible para nuestra nación y generaciones posteriores; satisfaciendo las necesidades del presente, sin poner en riesgo los recursos del futuro.

  3. 7th International Symposium on Ambient Intelligence

    CERN Document Server

    Paz, Juan; Novais, Paulo; Fernández-Caballero, Antonio; Yoe, Hyun; Ramírez, Andres; Villarrubia, Gabriel

    2016-01-01

    Ambient Intelligence (AmI) is a recent paradigm emerging from Artificial Intelligence, where computers are used as proactive tools assisting people with their day-to-day activities, making everyone’s life more comfortable. Another main concern of AmI originates from the human computer interaction domain and focuses on offering ways to interact with systems in a more natural way by means user friendly interfaces. This field is evolving quickly as can be witnessed by the emerging natural language and gesture based types of interaction. The inclusion of computational power and communication technologies in everyday objects is growing and their embedding into our environments should be as invisible as possible. In order for AmI to be successful, human interaction with computing power and embedded systems in the surroundings should be smooth and happen without people actually noticing it. The only awareness people should have arises from AmI: more safety, comfort and wellbeing, emerging in a natural and inherent...

  4. Ambient krypton-85 air sampling at Hanford

    International Nuclear Information System (INIS)

    Trevathan, M.S.; Price, K.R.

    1985-01-01

    In the fall of 1982, the Environmental Evaluations Section of Pacific Northwest Laboratory (PNL) initiated a network of continuous 85 Kr air samplers located on and around the Hanford Site. This effort was in response to the resumption of operations at a nuclear fuel reprocessing plant located onsite where 85 Kr was to be released during fuel dissolution. Preoperational data were collected using noble gas samplers designed by the Environmental Protection Agency-Las Vegas (EPA-LV). The samplers functioned erratically resulting in excessive maintenance costs and prompted a search for a new sampling system. State-of-the-art 85 Dr sampling methods were reviewed and found to be too costly, too complex and inappropriate for field application, so a simple bag collection system was designed and field tested. The system is composed of a reinforced, heavy plastic bag, connected to a variable flow pump and housed in a weatherproof enclosure. At the end of the four week sampling period the air in the bag is transferred by a compressor into a pressure tank for easy transport to the laboratory for analysis. After several months of operation, the air sampling system has proven its reliability and sensitivity to ambient levels of 85 Kr

  5. Ambient krypton-85 air sampling at Hanford

    International Nuclear Information System (INIS)

    Trevathan, M.S.; Price, K.R.

    1984-10-01

    In the fall of 1982, the Environmental Evaluations Section of Pacific Northwest Laboratory (PNL) initiated a network of continuous krypton-85 air samplers located on and around the Hanford Site. This effort was in response to the resumption of operations at a nuclear fuel reprocessing plant located onsite where krypton-85 was to be released during fuel dissolution. Preoperational data were collected using noble gas samplers designed by the Environmental Protection Agency-Las Vegas (EPA-LV). The samplers functioned erratically resulting in excessive maintenance costs and prompted a search for a new sampling system. State of the art krypton-85 sampling methods were reviewed and found to be too costly, too complex and inappropriate for field application, so a simple bag collection system was designed and field tested. The system is composed of a reinforced, heavy plastic bag, connected to a variable flow pump and housed in a weatherproof enclosure. At the end of the four week sampling period the air in the bag is transferred by a compressor into a pressure tank for easy transport to the laboratory for analysis. After several months of operation, the air sampling system has proven its reliability and sensitivity to ambient levels of krypton-85. 3 references, 3 figures, 1 table

  6. 4th International Symposium on Ambient Intelligence

    CERN Document Server

    Hallenborg, Kasper; Rodríguez, Juan; Tapia, Dante; Novais, Paulo

    2013-01-01

    Ambient Intelligence (AmI) is a recent paradigm emerging from Artificial Intelligence (AI), where computers are used as proactive tools assisting people with their day-to-day activities, making everyone’s life more comfortable. Another main concern of AmI originates from the human computer interaction domain and focuses on offering ways to interact with systems in a more natural way by means user friendly interfaces. This field is evolving quickly as can be witnessed by the emerging natural language and gesture based types of interaction. The inclusion of computational power and communication technologies in everyday objects is growing and their embedding into our environments should be as invisible as possible. In order for AmI to be successful, human interaction with computing power and embedded systems in the surroundings should be smooth and happen without people actually noticing it. The only awareness people should have arises from AmI: more safety, comfort and wellbeing, emerging in a natural and inh...

  7. 3rd International symposium on ambient intelligence

    CERN Document Server

    Tapia, Dante I; Novais, Paulo; ISAmI 2012; Ambient Intelligence : Software and Applications

    2012-01-01

    Ambient Intelligence (AmI) is a recent paradigm emerging from Artificial Intelligence (AI), where computers are used as proactive tools assisting people with their day-to-day activities, making everyone’s life more comfortable. Another main concern of AmI originates from the human computer interaction domain and focuses on offering ways to interact with systems in a more natural way by means user friendly interfaces. This field is evolving quickly as can be witnessed by the emerging natural language and gesture based types of interaction. The inclusion of computational power and communication technologies in everyday objects is growing and their embedding into our environments should be as invisible as possible. In order for AmI to be successful, human interaction with computing power and embedded systems in the surroundings should be smooth and happen without people actually noticing it. The only awareness people should have arises from AmI: more safety, comfort and wellbeing, emerging in a natural and inh...

  8. POLÍTICA PÚBLICA AMBIENTAL Y AMBIENTE EN EL VALLE DEL CAUCA, 1991-2010

    Directory of Open Access Journals (Sweden)

    Álvaro Quiceno Martínez

    2010-01-01

    Full Text Available La política pública ambiental en el Valle del Cauca, a partir de 1991, va en contravía de la conservación ambiental al enfatizar en el crecimiento económico, la generación de plusvalía y la ampliación de la tasa de ganancia. Para la realización de esta investigación se adoptó como herramienta metodológica la revisión bibliográfica. Se halló que la política pública ambiental en el Valle se ha orientado a subsidiar monetaria, ecológica, ambiental y socialmente al sector cañicultor. Se concluye que dicha política ha contribuido al desequilibrio ecológico y al deterioro ambiental.

  9. Farelo de trigo e complexo enzimático na alimentação de poedeiras semipesadas na fase de produção Wheat bran and enzymatic complex in the feeding of laying hens in the production phase

    Directory of Open Access Journals (Sweden)

    Daniel de Magalhães Araujo

    2008-05-01

    Full Text Available O estudo foi conduzido com o objetivo de avaliar os efeitos da inclusão de farelo de trigo (FT na ração com ou sem a suplementação de um complexo enzimático (CE sobre o desempenho e a produção de ovos de frangas semipesadas na fase de produção. Foram utilizadas 288 frangas Lohmann Brown, distribuídas em delineamento inteiramente casualizado, em esquema fatorial 4 × 2, composto de quatro níveis de farelo de trigo (0, 3, 6 e 9% na ração e da suplementação de complexo enzimático (0 ou 100 g/100 kg de ração, com oito tratamentos e seis repetições de seis aves. O complexo enzimático continha as enzimas beta-galactosidase, galactomananase, xilanase e alfa-glucanase. O consumo de ração, o peso vivo final, a produção de ovos, o peso e a massa de ovos e a conversão por massa e por dúzia de ovos não foram afetados pela inclusão do farelo de trigo nas rações. A gravidade específica da casca dos ovos piorou com o aumento dos níveis de farelo de trigo nas dietas. Nenhuma característica foi afetada pela adição do complexo enzimático na ração, com exceção do peso do ovo, que melhorou de 62,74 para 64,28 g. Recomenda-se o uso de até 9% de farelo de trigo em rações para poedeiras em produção. A adição de alfa-galactosidase, galactomananase, xilanase e alfa-glucanase aumenta o peso dos ovos.The study was carried out with the objective to evaluate the effects of the inclusion of the wheat bran (WB with or without supplementation of an enzymatic complex (EC on the performance of semi-heavy hens in the egg-production phase. A total of 288 Lohmann Brown pullets were used, distributed to a completely randomized design in 4 × 2 factorial arrangement, composed by four WB levels (0, 3, 6 and 9% in the ration and enzymatic complex supplementation (0 or 100g/100 kg diet, with eight treatments and six replicates of six birds. The enzymatic complex contained the enzymes beta-galactosidase, galactomananase, xilanase and alpha

  10. Influência do tamanho das partículas na solubilização enzimática da proteína do farelo de soja = The influence of particle size in the enzymatic extraction of soybean meal protein

    OpenAIRE

    Maura Stenzel; Ana Kelly Girata; Nehemias Curvelo Pereira; Gisella Maria Zanin; Oswaldo Curty Motta Lima

    2009-01-01

    O objetivo deste trabalho foi verificar a influência do tamanho das partículas na quantidade de proteína solubilizada a partir do farelo de soja. O farelo foi peneirado e cada fração foi submetida à hidrólise enzimática com as proteases Alcalase e Flavourzyme. Ashidrólises foram realizadas a 60ºC, agitação de 100 rpm, concentração de Alcalase 0,5% (proteína/proteína) por 3h e concentração de Flavourzyme 1% (proteína/proteína) por 5h. A quantidade de proteína solubilizada foi determinada pelo ...

  11. Estudio de laboratorio del efecto de las concentraciones de calcio y caseína, el pH y la temperatura sobre la incorporación de proteínas lácteas al coágulo obtenido por acción enzimática

    Directory of Open Access Journals (Sweden)

    Pires Miryam S.

    1999-01-01

    Full Text Available The effect of casein concentration, Ca2+ concentration, temperature and pH on the amount and size of protein aggregates (fines in the whey produced by enzimic coagulation of nonfat milk was studied in laboratory conditions. Casein concentrations about 0.3 g/L showed a minimal amount of caseins in the whey, with presence of small aggregates of casein micellles. Ca2+ concentrations higher than 5 mM were neccesary to reduce the whey protein to a minimum constituted by protein particles smaller than casein micelles. The coagulation temperature, in the 35 - 45oC range, produced almost no variations in the whey proteins. The obtention of a minimum amount of whey proteins was possible only in a narrow pH range around 6.4. These results pointed to casein concentration and pH as important variables to be controlled in connection with the process yield.

  12. Períodos de digestão enzimática para o resgate de folículos pré-antrais em ovários de fetos bovinos Periods of enzimatic digestion for rescueing preantral follicles from fetal bovine ovaries

    Directory of Open Access Journals (Sweden)

    Silvia Ferreira Carambula

    1998-12-01

    Full Text Available O presente experimento foi delineado com o objetivo de determinar a influência de diferentes períodos de digestão enzimática nos ovários de fetos bovinos, sobre o número total de folículos pré-antrais resgatados e o número de folículos resgatados em cada estágio de desenvolvimento. A presença da membrana basal, envolvendo o complexo formado pelas células da granulosa e o oócito, foi observada através de um estudo histológico, o que permitiu avaliar a integridade morfológica dos folículos pré-antrais isolados. Para isso, ovários de fetos bovinos, entre 150 e 270 dias de gestação, foram obtidos em frigorífico. No laboratório, os ovários foram seccionados em vários fragmentos com uma tesoura cirúrgica e dissociados com pipetas de Pasteur, com diâmetros aproximados de 1000 e 500mim. Após este processo, os fragmentos foram submetidos à digestão enzimática com colagenase em uma concentração de 200UI/ml de TCM199 modificado, por períodos de 20, 30 ou 40 minutos. Os resultados obtidos com esta técnica permitiram determinar que o número de folículos pré-antrais isolados, assim como, os estágios de desen- volvimento em que esses folículos encontravam-se no momento do isolamento, são similares nos três períodos de incubação enzimática. A estrutura morfológica desses folículos, formada pelo oócito, células da granulosa e membrana basal, manteve-se intacta após o isolamento nos três períodos de digestão enzimática.The objective of this experiment was to determine the efficiency and the effect of different enzymatic digestion periods on the number and developmental stages of preantral follicles isolated from bovine fetal ovaries. The presence of basal membrane, surrounding granulosa cells-oocyte complex, was observed, after digestion, through histologycal study. For this, ovaries from bovine fetues, were collected in slaughterhouses, between 150 and 270 days of pregnancy. In the laboratory, the ovaries

  13. Efeito da administração de propileno glicol e cobalto associado à vitamina B12 sobre o perfil metabólico e a atividade enzimática de ovelhas da raça Santa Inês no periparto

    Directory of Open Access Journals (Sweden)

    Rogério Adriano dos Santos

    2012-12-01

    Full Text Available O presente estudo teve por objetivo avaliar a influência da administração de propileno glicol e cobalto associado à vitamina B12 sobre o perfil metabólico e a atividade enzimática de ovelhas da raça Santa Inês no período do periparto. Foram utilizadas 18 ovelhas prenhes, pesando em torno de 40kg. Aproximadamente 30 dias antes da data prevista para o parto foram separadas de maneira aleatória em três grupos e administrados os suplementos conforme a seguir: (G1/n=6 grupo que recebeu propileno glicol (30mL por via oral diariamente; (G2/n=6 grupo que recebeu cobalto (1mg de cloreto de cobalto a 1%, via oral diariamente associado a vitamina B12 (2mg via intramuscular, semanalmente e (G3/n=6 grupo controle. As amostras de sangue das ovelhas para avaliação do perfil metabólico e enzimático (glicose, β-hidroxibutirato-BHB, NEFA, proteína total, albumina, uréia, creatinina, AST, GGT, FA e CK foram colhidas 30 dias antes da data prevista para o parto, uma semana antes (ante-parto, no parto, às 24h, 72h, 5 dias, 15 dias e 30 dias após o parto. Não foi observado cetonúria nos momentos que antecederam ao parto. A administração dos suplementos não influenciou sobre o perfil metabólico, protéico e energético, assim como não houve comprometimento hepático das ovelhas no período do periparto.

  14. Utilização de dietas úmidas e de rações e água de bebida com edulcorante para leitões desmamados aos 21 dias de idade e efeitos sobre o desenvolvimento histológico e enzimático intestinal

    Directory of Open Access Journals (Sweden)

    Silva Caio Abércio da

    2001-01-01

    Full Text Available Um experimento foi realizado com leitões desmamados visando avaliar os efeitos de oito tratamentos, correspondentes à combinação dos fatores, apresentações de ração (seca e úmida, tipos de ração (sem e com edulcorante e tipos de água de consumo (sem e com edulcorante sobre o desenvolvimento morfológico e enzimático intestinal. Foram avaliadas a altura das vilosidades (V, a profundidade das criptas (C e a relação altura das vilosidades e profundidade das criptas (V:C do duodeno e do jejuno, o conteúdo de proteína na mucosa do jejuno, e as atividades enzimáticas (UA da maltase, sacarase e dipeptidase. Utilizaram-se 32 leitões machos castrados, Large White x Landrace, desmamados aos 21 dias, submetidos aos tratamentos durante oito dias. O delineamento experimental foi em blocos casualizados, com oito tratamentos, fatorial 2 x 2 x 2, com quatro repetições. Foram observadas diferenças para a altura das vilosidades do duodeno para os grupos tratados com dieta úmida e ração com edulcorante e para a relação V:C do duodeno para os animais que receberam ração com edulcorante. Houve diferença para a UA da sacarase e da maltase para os grupos que receberam água sem edulcorante. As características histológicas foram favorecidas pelas dietas úmidas, o que pode sugerir que esta apresentação foi menos agressiva ao trato digestivo. Para os demais fatores os resultados contrariaram as expectativas de que o tratamento água com edulcorante melhoraria a qualidade do epitélio intestinal. A fase crítica pela qual os leitões foram submetidos, possivelmente, comprometeu os resultados.

  15. Radon-222 related influence on ambient gamma dose.

    Science.gov (United States)

    Melintescu, A; Chambers, S D; Crawford, J; Williams, A G; Zorila, B; Galeriu, D

    2018-04-03

    Ambient gamma dose, radon, and rainfall have been monitored in southern Bucharest, Romania, from 2010 to 2016. The seasonal cycle of background ambient gamma dose peaked between July and October (100-105 nSv h -1 ), with minimum values in February (75-80 nSv h -1 ), the time of maximum snow cover. Based on 10 m a.g.l. radon concentrations, the ambient gamma dose increased by around 1 nSv h -1 for every 5 Bq m -3 increase in radon. Radon variability attributable to diurnal changes in atmospheric mixing contributed less than 15 nSv h -1 to the overall variability in ambient gamma dose, a factor of 4 more than synoptic timescale changes in air mass fetch. By contrast, precipitation-related enhancements of the ambient gamma dose were 15-80 nSv h -1 . To facilitate routine analysis, and account in part for occasional equipment failure, an automated method for identifying precipitation spikes in the ambient gamma dose was developed. Lastly, a simple model for predicting rainfall-related enhancement of the ambient gamma dose is tested against rainfall observations from events of contrasting duration and intensity. Results are also compared with those from previously published models of simple and complex formulation. Generally, the model performed very well. When simulations underestimated observations the absolute difference was typically less than the natural variability in ambient gamma dose arising from atmospheric mixing influences. Consequently, combined use of the automated event detection method and the simple model of this study could enable the ambient gamma dose "attention limit" (which indicates a potential radiological emergency) to be reduced from 200 to 400% above background to 25-50%. Copyright © 2018 Elsevier Ltd. All rights reserved.

  16. Avaliação fenotípica da enzima Klebsiella pneumoniae carbapenemase (KPC em Enterobacteriaceae de ambiente hospitalar Phenotypic research on Klebsiella pneumoniae carbapenemase (KPC enzyme in Enterobacteriaceae from hospitals

    Directory of Open Access Journals (Sweden)

    Rosabel Dienstmann

    2010-02-01

    Full Text Available INTRODUÇÃO E OBJETIVO: A resistência bacteriana é problema frequente e importante no ambiente nosocomial. Nesse contexto, várias bactérias apresentam habilidade de desenvolver mecanismos de resistência enzimáticos, destacando-se as Enterobacteriaceae. Nesta família de microrganismos, a produção de Klebsiella pneumoniae carbapenemase (KPC é um mecanismo emergente, o que justifica sua vigilância constante. MATERIAL E MÉTODO: Este trabalho pesquisou o fenótipo de KPC em 30 isolados clínicos de enterobactérias resistentes a cefalosporinas de terceira geração e sensibilidade diminuída a carbapenem oriundas de dois hospitais (em Porto Alegre e na Grande Porto Alegre, RS. Realizou-se discodifusão com imipenem, meropenem e ertapenem, e 14 cepas com halo INTRODUCTION AND OBJECTIVE: Bacterial resistance is a frequent and important problem in the nosocomial environment. In this context, several bacteria have the ability to develop mechanisms of enzymatic resistance, mainly Enterobacteriaceae. In this family of microorganisms, the production of Klebsiella pneumoniae carbapenemase (KPC is an emerging mechanism, which should be under constant observation. MATERIAL AND METHOD: This study investigated the phenotype of KPC in 30 clinical isolates of Enterobacteriaceae resistant to third generation cephalosporin and carbapenem from two hospitals (Porto Alegre city and Porto Alegre, RS. It was performed disk diffusion method with imipenem, meropenem and ertapenem. Additionally, 14 strains with halo < 22 mm for the last antimicrobial agent underwent modified Hodge test. RESULTS: No sample showed carbapenemase (Hodge negative. DISCUSSION: Despite the fact there was no carbapenemase, resistance to carbapenems is possibly attributed to the presence of beta-lactamases AmpC and/or ESBL associated with changes in the permeability of porin channels. CONCLUSION: Given the emerging nature of KPC, it is important to trace it in Enterobacteriaceae

  17. Mapas de ruido: mecanismo de control del ruido ambiental

    OpenAIRE

    Chávez García, Kelvin

    2012-01-01

    Los primeros mecanismos de control del ruido ambiental fueron los mapas acústicos. La Directiva Europea 2002149/CE del Parlamento Europeo y del Consejo de Evatuacián y Gestión del Ruido Ambiental, exige, a los estados miembros,  se midan los niveles de ruido, en su entorno local, con  el fin de contar con indicadores de ruido comunes. elaborar mapas de ruido y planes de acción destinados a reducir los efectos nocivos de ruido ambiental.  Las directrices para la elaboración de l...

  18. Dependence of boron cluster dissolution on the annealing ambient

    International Nuclear Information System (INIS)

    Radic, Ljubo; Lilak, Aaron D.; Law, Mark E.

    2002-01-01

    Boron is introduced into silicon via implantation to form p-type layers. This process creates damage in the crystal that upon annealing causes enhanced diffusion and clustering of the boron layer. Reactivation of the boron is not a well-understood process. In this letter we experimentally investigate the effect of the annealing ambient on boron reactivation kinetics. An oxidizing ambient which injects silicon interstitials is compared to an inert ambient. Contrary to published theory, an excess of interstitials does not accelerate the reactivation process

  19. Radionuclide Transport Models Under Ambient Conditions

    International Nuclear Information System (INIS)

    Moridis, G.; Hu, Q.

    2000-01-01

    The purpose of this Analysis/Model Report (AMR) is to evaluate (by means of 2-D semianalytical and 3-D numerical models) the transport of radioactive solutes and colloids in the unsaturated zone (UZ) under ambient conditions from the potential repository horizon to the water table at Yucca Mountain (YM), Nevada. This is in accordance with the ''AMR Development Plan U0060, Radionuclide Transport Models Under Ambient Conditions'' (CRWMS M and O 1999a). This AMR supports the UZ Flow and Transport Process Model Report (PMR). This AMR documents the UZ Radionuclide Transport Model (RTM). This model considers: the transport of radionuclides through fractured tuffs; the effects of changes in the intensity and configuration of fracturing from hydrogeologic unit to unit; colloid transport; physical and retardation processes and the effects of perched water. In this AMR they document the capabilities of the UZ RTM, which can describe flow (saturated and/or unsaturated) and transport, and accounts for (a) advection, (b) molecular diffusion, (c) hydrodynamic dispersion (with full 3-D tensorial representation), (d) kinetic or equilibrium physical and/or chemical sorption (linear, Langmuir, Freundlich or combined), (e) first-order linear chemical reaction, (f) radioactive decay and tracking of daughters, (g) colloid filtration (equilibrium, kinetic or combined), and (h) colloid-assisted solute transport. Simulations of transport of radioactive solutes and colloids (incorporating the processes described above) from the repository horizon to the water table are performed to support model development and support studies for Performance Assessment (PA). The input files for these simulations include transport parameters obtained from other AMRs (i.e., CRWMS M and O 1999d, e, f, g, h; 2000a, b, c, d). When not available, the parameter values used are obtained from the literature. The results of the simulations are used to evaluate the transport of radioactive solutes and colloids, and

  20. El atal como ambiente de aprendizaje inclusivo

    Directory of Open Access Journals (Sweden)

    Inmaculada González Falcón

    2017-04-01

    Full Text Available En las últimas décadas la creciente llegada de población inmigrantes en España ha generado la determinación de nuevas políticas educativas de escolarización para el alumnado de origen extranjero. En la comunidad andaluza, se destaca la creación del “Aula Temporal de Aceleración Lingüística” para la promoción de la enseñanza de la lengua vehicular y la integración socioeducativa del alumnado de origen extranjero. El trabajo que aquí se presenta forma parte de proyecto nacional I+D+I (Ref. CSO2013-43266-R sobre las trayectorias de estos dispositivos. Este artículo se formula bajo un doble objetivo: por un lado, analizar las variables didácticas que se desarrollan en las ATAL de dos centros públicos de educación secundaria de la provincia onubense; y por otro, valorar las semejanzas y disonancias entre dichas aulas a través de un estudio etnográfico, explicitando de igual modo los condicionantes en cuanto a tres ejes de análisis: física-funcional, temporal y relacional. Los resultados se dirigen hacia el reconocimiento del aula como espacio de apoyo emocional y de atención personal desde la configuración de ambientes de aprendizaje. Este hecho evidencia la reflexión hacia modelos educativos que conformen un marco de actuación intercultural como generadores de la inclusión para el alumnado extranjero en los centros educativos.

  1. Advances in ambient temperature secondary lithium cells

    Science.gov (United States)

    Subbarao, S.; Shen, D. H.; Deligiannis, F.; Huang, C-K.; Halpert, G.

    1989-01-01

    The Jet Propulsion Laboratory is involved in a Research and Development program sponsored by NASA/OAST on the development of ambient temperature secondary lithium cells for future space applications. Some of the projected applications are planetary spacecraft, planetary rovers, and astronaut equipment. The main objective is to develop secondary lithium cells with greater than 100 Wh/kg specific energy while delivering 1000 cycles at 50 percent Depth of Discharge (DOD). To realize these ambitious goals, the work was initially focused on several important basic issues related to the cell chemistry, selection of cathode materials and electrolytes, and component development. The performance potential of Li-TiS2, Li-MoS3, Li-V6O13 and Li-NbSe3 electrochemical systems was examined. Among these four, the Li-TiS2 system was found to be the most promising system in terms of realizable specific energy and cycle life. Some of the major advancements made so far in the development of Li-TiS2 cells are in the areas of cathode processing technology, mixed solvent electrolytes, and cell assembly. Methods were developed for the fabrication of large size high performance TiS2 cathodes. Among the various electrolytes examined, 1.5M LiAsF6/EC + 2-MeTHF mixed solvent electrolyte was found to be more stable towards lithium. Experimental cells activated with this electrolyte exhibited more than 300 cycles at 100 percent Depth of Discharge. Work is in progress in other areas such as selection of lithium alloys as candidate anode materials, optimization of cell design, and development of 5 Ah cells. The advances made at the Jet Propulsion Laboratory on the development of secondary lithium cells are summarized.

  2. Do ambient urban odors evoke basic emotions?

    Directory of Open Access Journals (Sweden)

    Sandra Theresia Weber-Glass

    2014-04-01

    Full Text Available Fragrances, such as plant odors, have been shown to evoke autonomic response patterns associated with Ekman’s (Ekman et al., 1983 basic emotions happiness, surprise, anger, fear, sadness and disgust. Inducing positive emotions by odors in highly frequented public spaces could serve to improve the quality of life in urban environments. Thus, the present study evaluated the potency of ambient odors connoted with an urban environment to evoke basic emotions on an autonomic and cognitive response level. Synthetic mixtures representing the odors of disinfectant, candles / bees wax, summer air, burnt smell, vomit and musty smell as well as odorless water as a control were presented five times in random order to 30 healthy, non-smoking human subjects with intact sense of smell. Skin temperature, skin conductance, breathing rate, forearm muscle activity, blink rate and heart rate were recorded simultaneously. Subjects rated the odors in terms of pleasantness, intensity and familiarity and gave verbal labels to each odor as well as cognitive associations with the basic emotions. The results showed that the amplitude of the skin conductance response varied as a function of odor presentation. Burnt smell and vomit elicited significantly higher electrodermal responses than summer air. Also, a negative correlation was revealed between the amplitude of the skin conductance response and hedonic odor valence indicating that the magnitude of the electrodermal response increased with odor unpleasantness. The analysis of the cognitive associations between odors and basic emotions showed that candles / bees wax and summer air were specifically associated with happiness whereas burnt smell and vomit were uniquely associated with disgust. Our findings suggest that city odors may evoke specific cognitive associations of basic emotions and that autonomic activity elicited by such odors is related to odor hedonics.

  3. Ambient Intelligence Systems for Personalized Sport Training

    Directory of Open Access Journals (Sweden)

    Daniel González-Jiménez

    2010-03-01

    Full Text Available Several research programs are tackling the use of Wireless Sensor Networks (WSN at specific fields, such as e-Health, e-Inclusion or e-Sport. This is the case of the project “Ambient Intelligence Systems Support for Athletes with Specific Profiles”, which intends to assist athletes in their training. In this paper, the main developments and outcomes from this project are described. The architecture of the system comprises a WSN deployed in the training area which provides communication with athletes’ mobile equipments, performs location tasks, and harvests environmental data (wind speed, temperature, etc.. Athletes are equipped with a monitoring unit which obtains data from their training (pulse, speed, etc.. Besides, a decision engine combines these real-time data together with static information about the training field, and from the athlete, to direct athletes’ training to fulfill some specific goal. A prototype is presented in this work for a cross country running scenario, where the objective is to maintain the heart rate (HR of the runner in a target range. For each track, the environmental conditions (temperature of the next track, the current athlete condition (HR, and the intrinsic difficulty of the track (slopes influence the performance of the athlete. The decision engine, implemented by means of (m; s-splines interpolation, estimates the future HR and selects the best track in each fork of the circuit. This method achieves a success ratio in the order of 80%. Indeed, results demonstrate that if environmental information is not take into account to derive training orders, the success ratio is reduced notably.

  4. Reflexo do consumismo infantil no ambiente escolar

    Directory of Open Access Journals (Sweden)

    Sônia de Fátima Camargo

    2011-12-01

    Full Text Available Este artigo propôs verificar se há reflexos do consumismo infantil no ambiente escolar. Se á fatores que influenciam nas interações afetivas e subjetivas dos alunos das séries iniciais do Ensino Fundamental na Escola Municipal Professora Ana Cristina de sena município de Sinop – MT. O objetivo do trabalho foi investigar as relações interpessoais em sala de aula e de que forma essas relações acontecem diante do consumismo. Como fundamentação teórica, recorremos aos autores, Zygmunt Bauman, Anne Barrére, Antonio Chizotti e Maria Minayo. A metodologia usada consistiu na pesquisa qualitativa, no estudo de caso e entrevistas com perguntas abertas e fechadas aplicadas a professores e pais e observação em sala dos alunos. Através dos relatos dos sujeitos realizamos um estudo analítico confrontando os dados da pesquisa com os autores principais que fundamentam a mesma. O presente trabalho pretende contribuir com a escola na sensibilização de futuros consumidores, e como trabalhar com conceitos de valores do ‘ser’ e não do ‘ter’. Os resultados da investigação revelam que a realidade na qual foi pesquisado, o reflexo do consumismo e contundentes e que o consumismo não é o único fator a influenciar nas relações e formação subjetiva dos alunos.Palavras-chave: educação; ensino fundamental; anos iniciais; consumismo; sociointeracionismo.

  5. Educación ambiental en la crisis y crisis de la educación ambiental

    OpenAIRE

    Delgado Huertos, Enrique

    2012-01-01

    Producción Científica Es preciso repensar la Educación Ambiental una vez asumido que los ciudadanos despues de tres décadas de mensajes y actividades educativas, si bien han cambiado su percepción de los problemas ambientales, apenas ha variado su actitud y su compromiso personal en favor del medio ambiente. La educación ambiental ha de perder el carácter anecdótico y pasar de actividades aisladas, repetitivas y desconectadas del resto de los aprendizajes a una intervención basada en la si...

  6. Educação ambiental na escola: a perspectiva estudantil sobre o meio ambiente e a propaganda ambiental na internet

    Directory of Open Access Journals (Sweden)

    Fernanda Reis de Pinho Tavares

    2005-01-01

    Full Text Available Este trabalho visa mostrar as idéias dos estudantes sobre um tema - a propaganda - refletindo como ela se insere na questão ambiental. Também abordamos como os alunos percebem questões como as relações, empresa, meio ambiente e sociedade. Foi realizado em uma oficina de Educação Ambiental, em uma escola particular de Lagoa Santa - MG, durante os meses de junho e julho de 2004. Analisaram-se sites de diversas empresas e ONG's (Organizações Não Governamentais. Em sua maioria os estudantes conseguiram posicionar-se criticamente em relação às entidades investigadas.

  7. Sampling and analyte enrichment strategies for ambient mass spectrometry.

    Science.gov (United States)

    Li, Xianjiang; Ma, Wen; Li, Hongmei; Ai, Wanpeng; Bai, Yu; Liu, Huwei

    2018-01-01

    Ambient mass spectrometry provides great convenience for fast screening, and has showed promising potential in analytical chemistry. However, its relatively low sensitivity seriously restricts its practical utility in trace compound analysis. In this review, we summarize the sampling and analyte enrichment strategies coupled with nine modes of representative ambient mass spectrometry (desorption electrospray ionization, paper vhspray ionization, wooden-tip spray ionization, probe electrospray ionization, coated blade spray ionization, direct analysis in real time, desorption corona beam ionization, dielectric barrier discharge ionization, and atmospheric-pressure solids analysis probe) that have dramatically increased the detection sensitivity. We believe that these advances will promote routine use of ambient mass spectrometry. Graphical abstract Scheme of sampling stretagies for ambient mass spectrometry.

  8. MEDIO AMBIENTE, TUTELAS DE URGENCIA Y PROCESO COLECTIVO

    Directory of Open Access Journals (Sweden)

    Jeferson Marin

    2011-12-01

    Full Text Available A problemática ambiental vem assumindo grande importância na sociedade atual. A utilização demasiada dos recursos serve como base para grandes discussões. Ao mesmo tempo em que se reconhece ao cidadão o direito de usufruir do ambiente que o cerca, impõe deveres, no sentido de garantir-se a utilização racional dos recursos ambientais. Apesar da existência de uma vasta legislação ambiental, o tema exige maiores mecanismos de controle, visando a proteção e garantia dos recursos naturais. Assim, faz-se necessária uma nova interpretação dos princípios processuais que vigoram no ordenamento, no sentido de melhorar a tutela do bem ambiental.

  9. Superconductor shields test chamber from ambient magnetic fields

    Science.gov (United States)

    Hildebrandt, A. F.

    1965-01-01

    Shielding a test chamber for magnetic components enables it to maintain a constant, low magnetic field. The chamber is shielded from ambient magnetic fields by a lead foil cylinder maintained in a superconducting state by liquid helium.

  10. Human aspects in ambient intelligence contemporary challenges and solutions

    CERN Document Server

    Bosse, Tibor; Neerincx, Mark

    2013-01-01

    This book presents recent developments in the field of human aspects in Ambient Intelligence. It addresses multidisciplinary aspects of AmI with human-directed disciplines such as psychology, social science, neuroscience and biomedical sciences.

  11. Technology review: prototyping platforms for monitoring ambient conditions.

    Science.gov (United States)

    Afolaranmi, Samuel Olaiya; Ramis Ferrer, Borja; Martinez Lastra, Jose Luis

    2018-05-08

    The monitoring of ambient conditions in indoor spaces is very essential owing to the amount of time spent indoors. Specifically, the monitoring of air quality is significant because contaminated air affects the health, comfort and productivity of occupants. This research work presents a technology review of prototyping platforms for monitoring ambient conditions in indoor spaces. It involves the research on sensors (for CO 2 , air quality and ambient conditions), IoT platforms, and novel and commercial prototyping platforms. The ultimate objective of this review is to enable the easy identification, selection and utilisation of the technologies best suited for monitoring ambient conditions in indoor spaces. Following the review, it is recommended to use metal oxide sensors, optical sensors and electrochemical sensors for IAQ monitoring (including NDIR sensors for CO 2 monitoring), Raspberry Pi for data processing, ZigBee and Wi-Fi for data communication, and ThingSpeak IoT platform for data storage, analysis and visualisation.

  12. Volumetric ambient occlusion for real-time rendering and games.

    Science.gov (United States)

    Szirmay-Kalos, L; Umenhoffer, T; Toth, B; Szecsi, L; Sbert, M

    2010-01-01

    This new algorithm, based on GPUs, can compute ambient occlusion to inexpensively approximate global-illumination effects in real-time systems and games. The first step in deriving this algorithm is to examine how ambient occlusion relates to the physically founded rendering equation. The correspondence stems from a fuzzy membership function that defines what constitutes nearby occlusions. The next step is to develop a method to calculate ambient occlusion in real time without precomputation. The algorithm is based on a novel interpretation of ambient occlusion that measures the relative volume of the visible part of the surface's tangent sphere. The new formula's integrand has low variation and thus can be estimated accurately with a few samples.

  13. RadNet (Environmental Radiation Ambient Monitoring System)

    Data.gov (United States)

    U.S. Environmental Protection Agency — RadNet, formerly Environmental Radiation Ambient Monitoring System (ERAMS), is a national network of monitoring stations that regularly collect air, precipitation,...

  14. A proposal of ubiquitous fuzzy computing for ambient Intelligence

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.

    2008-01-01

    Ambient Intelligence is considered as the composition of three emergent technologies: Ubiquitous Computing, Ubiquitous Communication and Intelligent User Interfaces. The aim of integration of aforesaid technologies is to make wider the interaction between human beings and information technology

  15. Ubiquitous fuzzy computing in open ambient intelligence environments

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.

    2006-01-01

    Ambient intelligence (AmI) is considered as the composition of three emergent technologies: ubiquitous computing, ubiquitous communication and intelligent user interfaces. The aim of integration of aforesaid technologies is to make wider the interaction between human beings and information

  16. Ambient intelligence & personalization : people's perspectives on information privacy

    NARCIS (Netherlands)

    Garde - Perik, van de E.M.

    2009-01-01

    Current developments towards Ambient Intelligence and related technological visions of the future are founded on continuous collection of information about individuals and their activities. This collection of information, its potentially persistent storage, dissemination and use raise privacy

  17. Arte-educação ambiental, um constructo transdisciplinar

    OpenAIRE

    Rache, Rita Patta

    2017-01-01

    A partir da práxis educativa de arte-­educadoras que desenvolvem ações articuladas entre arte-­ educação e educação ambiental, particularmente aquelas que contribuíram para a elaboração da metodologia de educação ambiental da ONG Núcleo de Educação e Monitoramento Ambiental (NEMA), de Rio Grande/RS, e da Escola da Natureza, de Brasília/DF, constatamos a emergência da expressão Arte-­Educação Ambiental (AEA). Nosso objetivo foi investigar o fenômeno, seu conteúdo, referenciais teóricos, as met...

  18. Responsabilidad ambiental: factor creador de valor agregado en las organizaciones

    Directory of Open Access Journals (Sweden)

    Alexa Senior

    2007-01-01

    Full Text Available Este artículo tiene como objetivo analizar la responsabilidad ambiental como oportunidad para crear valor en las organizaciones, en el entendido de que la misma permite cimentar las bases del desarrollo sustentable. Se considera igualmente la responsabilidad ambiental como elemento capaz de disminuir costos por riesgos y aumentar el "market share" a través de la innovación. Para lograr este objetivo se empleó la contrastación de teorías las cuales explican cómo la responsabilidad ambiental puede generar valor agregado en las organizaciones a partir de consideraciones tales como, ética ambiental, legitimidad y desarrollo sustentable. Se concluye que las empresas a través de sus esfuerzos socioambientales pueden obtener beneficios a la vez que mejoran su capital reputacional.

  19. Effects of elevated ambient temperature on embryo implantation in rats

    African Journals Online (AJOL)

    Yomi

    2012-03-22

    Mar 22, 2012 ... ambient temperature leads to a delayed implantation and reduced number of implantation sites in. Sprague ... rates decrease after exposure to stress. One of the ..... implantation initiation time, support the previous findings.

  20. Amplification and Attenuation across USArray using Ambient Noise Wavefront Tracking

    KAUST Repository

    Bowden, Daniel C.; Tsai, Victor C.; Lin, Fan-Chi

    2017-01-01

    As seismic travel-time tomography continues to be refined using data from the vast USArray dataset, it is advantageous to also exploit the amplitude information carried by seismic waves. We use ambient noise cross correlation to make observations

  1. THE GENOTOXICITY OF AMBIENT OUTDOOR AIR, A REVIEW: SALMONELLA MUTAGENICITY

    Science.gov (United States)

    The genotoxicity of ambient outdoor air, a review: Salmonella mutagenicityAbstractMutagens in urban air pollution come from anthropogenic sources (especially combustion sources) and are products of airborne chemical reactions. Bacterial mutation tests have been used ...

  2. A systemic domain model for ambient pervasive persuasive games

    OpenAIRE

    Eglin, Roger; Eyles, Mark; Dansey, Neil

    2008-01-01

    By the development of the system domain model it is hoped that a greater conceptual and theoretical clarity may be brought to understanding the complex and multifaceted nature of pervasive and ambient computer games. This paper presents a conceptual model, the system domain model, to illustrate domain areas that exist in a console, pervasive or ambient game. It is implicit that the regions that the systemic domain model describes are contextually dependent. By developing this model it is poss...

  3. Risk related behaviour under different ambient scent conditions

    OpenAIRE

    Alina Gagarina; Indre Pikturniene

    2016-01-01

    The article analyses the effect of two ambient scents (peppermint and vanilla) and their intensiveness on risk related behaviour that is expressed through selected decision making heuristics. Purpose of the article: The purpose of this article is to identify the relationship of ambient scent type and intensiveness with risk related behaviour that is expressed through selected decision making heuristics. Methodology/methods: 2×2 factorial experiment with control group was run. Ambi...

  4. Biorregionalismo, ética e justiça ambiental

    Directory of Open Access Journals (Sweden)

    Silvio Luiz Negrão

    2006-01-01

    Full Text Available Nesse artigo, retomo a argumentação de Gary Snyder, um libertário biorregionalista, e do socialista John Clark, verificando os limites e possibilidades de suas propostas na formulação de uma ética ambiental, à luz dos critérios estabelecidos por Kenneth Goodpaster (vida e por Tom Regan (valor inerente, para fundamentar uma ética genuinamente ambiental.

  5. Enhancing Safety of Artificially Ventilated Patients Using Ambient Process Analysis.

    Science.gov (United States)

    Lins, Christian; Gerka, Alexander; Lüpkes, Christian; Röhrig, Rainer; Hein, Andreas

    2018-01-01

    In this paper, we present an approach for enhancing the safety of artificially ventilated patients using ambient process analysis. We propose to use an analysis system consisting of low-cost ambient sensors such as power sensor, RGB-D sensor, passage detector, and matrix infrared temperature sensor to reduce risks for artificially ventilated patients in both home and clinical environments. We describe the system concept and our implementation and show how the system can contribute to patient safety.

  6. Impacto ambiental de una mina de mercurio abandonada

    OpenAIRE

    Álvarez Cuartas, María

    2016-01-01

    El objetivo principal de este Trabajo Fin de Máster es la evaluación del impacto ambiental de una explotación minera de mercurio en estado de abandono y de posible contaminación del medio ambiente. Una vez que se comprueba el elevado impacto debido al estado de abandono y a la alta contaminación, se ha elaborado una sencilla propuesta de restauración, barajado y comprando tres posibles alternativas.

  7. Model checking biological systems described using ambient calculus

    DEFF Research Database (Denmark)

    Mardare, Radu Iulian; Priami, Corrado; Qualia, Paola

    2005-01-01

    Model checking biological systems described using ambient calculus. In Proc. of the second International Workshop on Computational Methods in Systems Biology (CMSB04), Lecture Notes in Bioinformatics 3082:85-103, Springer, 2005.......Model checking biological systems described using ambient calculus. In Proc. of the second International Workshop on Computational Methods in Systems Biology (CMSB04), Lecture Notes in Bioinformatics 3082:85-103, Springer, 2005....

  8. The risk of melanoma associated with ambient summer ultraviolet radiation.

    Science.gov (United States)

    Pinault, Lauren; Bushnik, Tracey; Fioletov, Vitali; Peters, Cheryl E; King, Will D; Tjepkema, Michael

    2017-05-17

    Depletion of the ozone layer has meant that ambient ultraviolet radiation (UVR) has increased in recent decades. At the same time, the incidence of skin cancers, including melanoma, has risen. The relatively few large-scale studies that linked ambient UVR to melanoma found a trend toward rising incidence closer to the equator, where UVR estimates are highest. Similar research has not been conducted in Canada, where ambient UVR is generally lower than in countries further south. Modelled UVR data for the months of June through August during the 1980-to-1990 period were spatially linked in Geographic Information Systems to 2.4 million white members of the 1991 Canadian Census Health and Environment Cohort and tracked for melanoma diagnosis over an 18-year period (1992 to 2009). Standard Cox proportional hazards models were used to estimate melanoma risk associated with increases of ambient summer UVR, assigned by residence at baseline. Models were adjusted for age, sex and socioeconomic (SES) characteristics. Separate analyses by body site of melanoma were conducted. Effect modification of the association between ambient UVR and melanoma by sex, age, outdoor occupation and selected SES characteristics was evaluated. Differences of one standard deviation (446 J/m², or 7% of the mean) in average ambient summer UVR were associated with an increased hazard ratio (HR) for melanoma of 1.22 (95% CI: 1.19 to 1.25) when adjusting for sex, age and SES characteristics. The HR for melanoma in relative UVR (per 1 standard deviation) was larger for men (HR = 1.26; 95% CI: 1.21 to 1.30) than for women (HR = 1.17; 95% CI: 1.13 to 1.22). Ambient summer UVR is associated with a greater risk of melanoma among the white population, even in a country where most people live within a narrow latitudinal belt. A stronger association between melanoma and ambient UVR was evident among men and among people of lower SES.

  9. Secondary organic aerosols: Formation potential and ambient data

    DEFF Research Database (Denmark)

    Barthelmie, R.J.; Pryor, S.C.

    1997-01-01

    Organic aerosols comprise a significant fraction of the total atmospheric particle loading and are associated with radiative forcing and health impacts. Ambient organic aerosol concentrations contain both a primary and secondary component. Herein, fractional aerosol coefficients (FAC) are used...... in conjunction with measurements of volatile organic compounds (VOC) to predict the formation potential of secondary organic aerosols (SOA) in the Lower Fraser Valley (LEV) of British Columbia. The predicted concentrations of SOA show reasonable accord with ambient aerosol measurements and indicate considerable...

  10. Big Data, Smart Homes and Ambient Assisted Living

    OpenAIRE

    Vimarlund, V.; Wass, S.

    2014-01-01

    Objectives: To discuss how current research in the area of smart homes and ambient assisted living will be influenced by the use of big data. Methods: A scoping review of literature published in scientific journals and conference proceedings was performed, focusing on smart homes, ambient assisted living and big data over the years 2011-2014. Results: The health and social care market has lagged behind other markets when it comes to the introduction of innovative IT solutions and the market f...

  11. Medio ambiente en Europa: retos para un desarrollo sostenible

    OpenAIRE

    Santa María Beneyto, María Jesús

    2000-01-01

    Ofrece una completa visión sobre el grave deterioro ambiental que sufre nuestro planeta, reconociendo la necesidad política medioambiental eficaz que permita conseguir una mejor protección de los ecosistemas y una base para el desarrollo sostenible, mostrando una especial atención a la situación del medio ambiente en la Unión Europea.

  12. Health Effects of Ambient Air Pollution in Developing Countries

    OpenAIRE

    Mannucci, Pier Mannuccio; Franchini, Massimo

    2017-01-01

    The deleterious effects of ambient air pollution on human health have been consistently documented by many epidemiologic studies worldwide, and it has been calculated that globally at least seven million deaths are annually attributable to the effects of air pollution. The major air pollutants emitted into the atmosphere by a number of natural processes and human activities include nitrogen oxides, volatile organic compounds, and particulate matter. In addition to the poor ambient air quality...

  13. On the Reference State for Exergy when Ambient Temperature Fluctuates

    OpenAIRE

    Michel Pons

    2009-01-01

    Exergy (availability) is the amount of mechanical work that could be produced by reversible processes. This notion is revisited in the case when ambient temperature fluctuates. Simple examples are first considered, and then a theoretical approach is developed. It results that the most reliable way for combining entropy and total energy into an exergy function is a linear combination where entropy is multiplied by a constant temperature. It results that ambient air has non-zero exergy, but tha...

  14. Consideraciones sobre ética, derecho y ambiente

    Directory of Open Access Journals (Sweden)

    Pierre Foy Valencia

    2009-12-01

    Full Text Available El presente ensayo aborda la importancia que progresivamente está adquiriendo la ética ambiental en relación con las variadas formas del ejercicio contemporáneo del Derecho, así como de sus diversificadas áreas o especialidades y, por cierto, en particular respecto del Derecho Ambiental. Para ello, en primer lugar, identificaremos conceptualmente el escenario y el proceso de la ética ambiental como presupuesto condicionante que impacta en los múltiples mecanismos de actuación social —léase la política, la economía, la educación, los sistemas jurídicos, entre otros—.2 Luego, estableceremos algunas premisas sobre la responsabilidad profesional en particular del abogado y cómo estas deberían ser integradas en una perspectiva ético-ambiental o, si se quiere, basadas en una ética de la sostenibilidad. Finalmente, interpretaremos cómo tales consideraciones debieran articularse con el ejercicio de las disciplinas jurídicas, en especial con el Derecho Ambiental, enfatizando la cuestión de la corrupción con la preocupación ambiental.

  15. Medio ambiente y educación ambiental: representaciones sociales de los profesores en formación

    Directory of Open Access Journals (Sweden)

    Raúl Calixto Flores

    2010-06-01

    Full Text Available Una de las líneas actuales de investigación educativa que aporta elementos valiosos para comprender los procesos de formación de los sujetos es la que corresponde a las representaciones sociales. En este artículo se describen los aspectos centrales de la investigación Representaciones del medio ambiente de los estudiantes de la licenciatura en educación primaria. Con las representaciones los estudiantes discriminan espacio-temporalmente los elementos del medio ambiente, estableciendo correspondencias con las prácticas sociales. Entre los resultados de la investigación referida se encuentra la existencia de una relación entre las representaciones del medio ambiente y las corrientes de la educación ambiental.

  16. Medio ambiente y educación ambiental: representaciones sociales de los profesores en formación

    Directory of Open Access Journals (Sweden)

    Raúl Calixto Flores

    2010-01-01

    Full Text Available Una de las líneas actuales de investigación educativa que aporta elementos valiosos para comprender los procesos de formación de los sujetos es la que corresponde a las representaciones sociales. En este artículo se describen los aspectos centrales de la investigación Representaciones del medio ambiente de los estudiantes de la licenciatura en educación primaria. Con las representaciones los estudiantes discriminan espaciotemporalmente los elementos del medio ambiente, estableciendo correspondencias con las prácticas sociales. Entre los resultados de la investigación referida se encuentra la existencia de una relación entre las representaciones del medio ambiente y las corrientes de la educación ambiental.

  17. Los problemas de la Educación Ambiental: ¿es posible una Educación Ambiental integradora?

    OpenAIRE

    García, J. Eduardo

    2002-01-01

    En este trabajo se presentan diferentes problemas y carencias de la Educación Ambiental. Para el tratamiento de estos problemas se plantea una perspectiva integradora que supere los reduccionismos actualmente existentes

  18. Saúde e meio ambiente nas cidades: os desafios da saúde ambiental

    Directory of Open Access Journals (Sweden)

    Nelson Gouveia

    1999-02-01

    Full Text Available Dentro de alguns poucos anos, nosso planeta contará com mais habitantes em áreas urbanas do que em áreas rurais. A urbanização desenfreada, sem mecanismos regulatórios e de controle, típica dos países periféricos, trouxe consigo enormes repercussões na saúde da população. Problemas como a insuficiência dos serviços básicos de saneamento, coleta e destinação adequada do lixo e condições precárias de moradia, tradicionalmente relacionados com a pobreza e o subdesenvolvimento, somam-se agora à poluição química e física do ar, da água e da terra, problemas ambientais antes considerados "modernos". Novamente, é sobre as populações mais carentes que recai a maior parte dos efeitos negativos da urbanização, gerando uma situação de extrema desigualdade e iniqüidade ambiental e em saúde. Para reverter esse quadro é preciso que haja uma reincorporação das questões do meio ambiente nas políticas de saúde, e a integração dos objetivos da saúde ambiental numa ampla estratégia de desenvolvimento sustentável. Uma abordagem mais integrada, com mecanismos intersetoriais que possibilitem um diálogo amplo entre as partes, trará enormes benefícios na conquista de melhores condições de vida nas cidades. A saúde ambiental hoje tem o desafio de promover uma melhor qualidade de vida e saúde nas cidades e a oportunidade de enfrentar o absurdo quadro de exclusão social, sob a perspectiva da eqüidade.In a matter of few years there will be more urban than rural dwellers worldwide. The rapid urbanisation lacking adequate control and regulatory mechanisms typical of developing countries, brought along huge effects to the health of the population Environmental problems traditionally related to poverty and underdevelopment such as insufficient provision of sanitation services, waste collection and disposal, and precarious housing conditions, are now added to environmental problems considered of "modern life" such as the

  19. Brote del virus ISA: crisis ambiental y capacidad de la institucionalidad ambiental para manejar el conflicto

    Directory of Open Access Journals (Sweden)

    Beatriz Bustos

    2012-09-01

    Full Text Available La industria salmonera chilena vivió recientemente una crisis de magnitud a partir de la aparición del virus ISA. El artículo examina esta situación, concentrándose en el papel que desempeña la ciencia en las soluciones políticas propuestas, como caso para discutir la capacidad de los mecanismos de gobernanza ambiental neoliberal para solucionar crisis ambientales y económicas. La pregunta que guía el trabajo es: ¿cuál fue el rol de la ciencia en la política ambiental para la industria? El argumento promovido es que la crisis representa un momento de reestructuración de la relación sociedad-naturaleza, en el cual se redefinieron mecanismos de propiedad, control y acceso a recursos naturales, pero en el que, pese al discurso neoliberal de confianza en los expertos y la ciencia como elementos de toma de decisiones, la información científica tuvo escasa relevancia.The Chilean salmon industry recently experienced a crisis due to an outbreak of the ISA virus. The article examines the crisis focusing on the role of science in the policy solutions proposed, to discuss the real capacity of neoliberal environmental governance mechanisms to solve environmental and economic crisis. The question guiding this research is, which was the role of science in environmental policy createdfor the salmon industry? The argument is that the crisis represents a reestructuring of the relationship between society and nature through the redefinition of property rights, control and access to natural resources, but in which, in spite of a neoliberal discourse of trust in experts and science as elements of decision making, scientific information had little relevance.

  20. Ambient Temperature and Cerebrovascular Hemodynamics in the Elderly.

    Science.gov (United States)

    Pan, Wen-Chi; Eliot, Melissa N; Koutrakis, Petros; Coull, Brent A; Sorond, Farzaneh A; Wellenius, Gregory A

    2015-01-01

    Some prior studies have linked ambient temperature with risk of cerebrovascular events. If causal, the pathophysiologic mechanisms underlying this putative association remain unknown. Temperature-related changes in cerebral vascular function may play a role, but this hypothesis has not been previously evaluated. We evaluated the association between ambient temperature and cerebral vascular function among 432 participants ≥65 years old from the MOBILIZE Boston Study with data on cerebrovascular blood flow, cerebrovascular resistance, and cerebrovascular reactivity in the middle cerebral artery. We used linear regression models to assess the association of mean ambient temperature in the previous 1 to 28 days with cerebrovascular hemodynamics adjusting for potential confounding factors. A 10°C increase in the 21-day moving average of ambient temperature was associated with a 10.1% (95% confidence interval [CI], 2.2%, 17.3%) lower blood flow velocity, a 9.0% (95% CI, 0.7%, 18.0%) higher cerebrovascular resistance, and a 15.3% (95%CI, 2.7%, 26.4%) lower cerebral vasoreactivity. Further adjustment for ozone and fine particulate matter (PM2.5) did not materially alter the results. However, we found statistically significant interactions between ambient temperature and PM2.5 such that the association between temperature and blood flow velocity was attenuated at higher levels of PM2.5. In this elderly population, we found that ambient temperature was negatively associated with cerebral blood flow velocity and cerebrovascular vasoreactivity and positively associated with cerebrovascular resistance. Changes in vascular function may partly underlie the observed associations between ambient temperature and risk of cerebrovascular events.

  1. Plume Splitting in a Two-layer Stratified Ambient Fluid

    Science.gov (United States)

    Ma, Yongxing; Flynn, Morris; Sutherland, Bruce

    2017-11-01

    A line-source plume descending into a two-layer stratified ambient fluid in a finite sized tank is studied experimentally. Although the total volume of ambient fluid is fixed, lower- and upper-layer fluids are respectively removed and added at a constant rate mimicking marine outfall through diffusers and natural and hybrid ventilated buildings. The influence of the plume on the ambient depends on the value of λ, defined as the ratio of the plume buoyancy to the buoyancy loss of the plume as it crosses the ambient interface. Similar to classical filling-box experiments, the plume can always reach the bottom of the tank if λ > 1 . By contrast, if λ < 1 , an intermediate layer eventually forms as a result of plume splitting. Eventually all of the plume fluid spreads within the intermediate layer. The starting time, tv, and the ending time, tt, of the transition process measured from experiments correlate with the value of λ. A three-layer ambient fluid is observed after transition, and the mean value of the measured densities of the intermediate layer fluid is well predicted using plume theory. Acknowledgments: Funding for this study was provided by NSERC.

  2. APONTAMENTOS PARA CONSTRUÇÃO DA RACIONALIDADE AMBIENTAL

    Directory of Open Access Journals (Sweden)

    Munir Jorge Felício

    2015-12-01

    Full Text Available Os apontamentos para construção da racionalidade ambiental propostos nesse texto foram reunidos das contribuições presentes nas análises da fenomenologia, da epistemologia e da ontologia do ser social em Marx. Essa iniciativa almeja ampliar e aprofundar a compreensão da problemática ambiental cujas mudanças globais provocam desafios principalmente para comunidade científica. Trata-se de problematizar o desenvolvimento da racionalidade econômica, a partir das degradações dos recursos naturais engendradas pelo atual avanço do modelo de desenvolvimento socioeconômico. Modelo que se baseia na majoração da produtividade, se apóia na expansão tecnológica e se sustenta da exploração como se os recursos naturais fossem ainda considerados abundantes e ilimitados. A transição dessa racionalidade econômica para a racionalidade ambiental é um empreendimento buscado pelas possibilidades de conhecimento advindo das análises da fenomenologia. Entre essas possibilidades, a interdisciplinaridade indicada como necessária pela epistemologia ambiental, pode se tornar uma interessante estratégia de pesquisa, desde que, tenha como ponto de partida, as demandas externas onde se encontram os elementos da atual questão ambiental e de seus desdobramentos.

  3. Subjektivitas Dalam Iklan Ambient Media Miracle Aesthetic Clinic

    Directory of Open Access Journals (Sweden)

    Wegig Murwonugroho

    2015-09-01

    Full Text Available Subjektivitas di dalam iklan adalah kuasa yang dimiliki iklan untuk mengkonstruksi trend, kebenaran, hingga ideologi yang terjadi melalui alam bawah sadar. Iklan berfungsi menyampaikan pesan, yang direpresentasi-visualkan melalui metafora-metafora yang menjembatani antara gambaran imajinasi dan realitas. Ambient media merupakan media beriklan yang memanfaatkan lingkungan di mana iklan ditempatkan. Miracle Aesthetic Clinic (Miracle merepresentasi-visualkan iklan ambient media-nya dengan cara menghilangkan tampilan wajah kedua boneka perempuan. Wajah model iklan merupakan komoditi pencitraan yang pada umumnya memiliki nilai jual, namun dalam kedua iklan ini justeru ditenggelamkan ke dalam alas meja dan dinding tembok. Penelitian ini menggunakan metode penelitian Analisa Wacana Kritis untuk menganalisa subjektivitas di dalam pemaknaan ambient media. Subjektivitas iklan Miracle menyudutkan perempuan tidak dapat hadir, tersisih, menjadi sosok yang mengerikan di dalam tataran sosialnya ketika wajah berjerawat.  Iklan ambient media Miracle ini berhasil mengkonstruksi wacana trend baru kecantikan wajah yang tidak terbatas pada tolok ukur karakteristik kecantikan pada umumnya. Kata kunci: ambient media, subjektivitas, kuasa, kecantikan ideal perempuan.

  4. Relationship between prostate-specific antigen levels and ambient temperature

    Science.gov (United States)

    Ohwaki, Kazuhiro; Endo, Fumiyasu; Hattori, Kazunori; Muraishi, Osamu

    2014-07-01

    We examined the association between prostate-specific antigen (PSA) and daily mean ambient temperature on the day of the test in healthy men who had three annual checkups. We investigated 9,694 men who visited a hospital for routine health checkups in 2007, 2008, and 2009. Although the means and medians of ambient temperature for the three years were similar, the mode in 2008 (15.8 °C) was very different from those in 2007 and 2009 (22.4 °C and 23.2 °C). After controlling for age, body mass index, and hematocrit, a multiple regression analysis revealed a U-shaped relationship between ambient temperature and PSA in 2007 and 2009 ( P 2.5 ng/mL) by ambient temperature, with the lowest likelihood of having a high PSA at 17.8 °C in 2007 ( P = 0.038) and 15.5 °C in 2009 ( P = 0.033). When tested at 30 °C, there was a 57 % excess risk of having a high PSA in 2007 and a 61 % higher risk in 2009 compared with those at each nadir temperature. We found a U-shaped relationship between PSA and ambient temperature with the lowest level of PSA at 15-20 °C.

  5. PERCEPCIÓN AMBIENTAL EN ESTUDIANTES DE SECUNDARIA

    Directory of Open Access Journals (Sweden)

    Benito Zamorano González

    2009-01-01

    Full Text Available Se investigó un grupo de 254 estudiantes de una secundaria pública de Matamoros, Tamaulipas, México, adaptando el cuestionario utilizado por el Grup de Recerca Educació i Ciutadani (GREIC de la Universitat de les Illes Balears. El estudio se basa en un conjunto de 39 preguntas distribuidas en cuatro secciones: Afirmaciones relacionadas con el medio ambiente, para indagar su postura; Preocupación por el medio ambiente, para identificar el lugar que genera mayor interés; Problemas ambientales, sirvió para identificar el conocimiento de la problemática que afecta a su región y la cuarta sección Acciones para hacer un mundo mejor, para identificar la disposición de los estudiantes para mejorar el ambiente. La validación del cuestionario adaptado refiere un coeficiente del Alfa de Cronbach de 0.862 y los datos en estudio representan de forma generalizada la percepción ambiental que tienen los estudiantes y manifiestan la importancia de la educación ambiental.

  6. La cultura ambiental. Una mirada desde la comunidad Venceremos

    Directory of Open Access Journals (Sweden)

    Msc. Bertha Nudis Ferrer-Hechavarría

    2015-10-01

    Full Text Available Este trabajo constituye una aproximación al estudio de la cultura ambiental como elemento necesario en el camino al desarrollo comunitario. Parte de un breve análisis conceptual de la cultura para luego llegar a la cultura ambiental de acuerdo con las consideraciones de la autora. Momento singular resulta el que destaca la necesidad de que las comunidades enfrenten los problemas ambientales en el camino al desarrollo y sean depositarias de una cultura ambiental que lo posibilite. En este empeño tiene gran importancia la implementación de proyectos comunitarios, pues las comunidades constituyen grupos sociales claves en la solución de los problemas ambientales a nivel global y local. Teniendo en cuenta lo anterior, ha sido diseñado y ya se implementa un proyecto de educación ambiental para el desarrollo local sostenible en una comunidad que lleva como nombre Venceremos; tiene, entre sus distintas líneas de desarrollo, a la cultura ambiental.

  7. Freqüência e atividade enzimática de Candida spp. na cavidade oral de pacientes diabéticos do serviço de endocrinologia de um hospital de Fortaleza-CE Frequency and enzymatic activity of Candida spp. oral cavity of diabetic patients of the service of endocrinology of a hospital of Fortaleza-CE

    Directory of Open Access Journals (Sweden)

    Everardo Albuquerque Menezes

    2007-08-01

    Full Text Available O diabetes mellitus (DM, uma doença endócrino-metabólica de alta e crescente prevalência, é citada como responsável pela ocorrência das candidíases orais. A candidíase constitui um espectro de infecções causadas por fungos do gênero Candida, sendo o seu agente mais comum a Candida albicans, embora outras espécies tenham sido identificadas (Candida tropicalis, Candida guillermondii, Candida glabrata, Candida krusei. O objetivo deste trabalho foi avaliar a freqüência e a atividade enzimática de Candida spp. na cavidade oral de pacientes diabéticos atendidos no Serviço de Endocrinologia do Hospital Universitário Walter Cantídio da Universidade Federal do Ceará (HUWC/UFC. Foram coletadas amostras de 48 pacientes diabéticos, de ambos os sexos, com situações variáveis de controle glicêmico. Os materiais clínicos foram colhidos com ajuda de swabs e semeados em placas de Petri contendo ágar-Sabouraud dextrose com cloranfenicol e incubado a 37°C. Os crescimentos foram identificados pelas provas clássicas usadas em micologia. Depois, essas cepas de Candida foram submetidas a provas de detecção de enzimas fosfolipase e proteinase. Destas, 15 amostras (31,25% apresentaram cultura positiva para o gênero Candida. A espécie mais freqüente foi a C. albicans, com 80%, seguida de C. tropicalis (13,3% e C. guillermondii (6,7%. Quanto à pesquisa da atividade enzimática de Candida spp., foi observado que 86,6% delas apresentaram atividade de proteinase e 80%, de fosfolipase. Conclui-se com tais resultados que a C. albicans é a mais freqüente e que as espécies de Candida isoladas possuem fortes atividades enzimáticas.Diabetes mellitus, a endocrine-metabolic disease, of high and increasing prevalence, is cited as responsible by the occurrence of oral candidiasis. Candidiasis constitutes a specter of infections caused by fungi of genera Candida; the most common agent is Candida albicans, but other species have also been

  8. Produção de complexos enzimáticos por A. niger a partir de soja por fermentação em estado sólido=Enzyme complexes production by A. niger from soybean under solid state fermentation

    Directory of Open Access Journals (Sweden)

    Salah Din Mahmud Hasan

    2012-04-01

    Full Text Available A soja possui ampla disponibilidade de componentes bioquímicos como proteínas, polissacarídeos, lipídios, carboidratos, minerais, vitaminas, fibras, lecitina, dentre outros, tornando interessante sua utilização como substrato para a fermentação em estado sólido (FES. Este processo, apesar de não ser tão utilizado industrialmente quanto à fermentação submersa (Fsm, apresenta-se viável, pois tem apresentado resultados superiores de produtividade, principalmente no cultivo de fungos filamentosos e na produção de enzimas. Assim, o objetivo deste trabalho foi o de comparar a produção de diferentes tipos de complexos enzimáticos por FES utilizando diferentes tipos de sojas (orgânica, transgênica e convencional com o fungo Aspergillus niger. Realizou-se a caracterização dos substratos para a produção de amilase, protease e lipase, avaliação das curvas de crescimento do microrganismo e das condições ótimas de produção da protease utilizando planejamento de experimentos. As maiores atividades enzimáticas no estudo dos parâmetros da fermentação foram observadas para a protease, utilizando soja convencional com umidade inicial de 50%, 144h de fermentação, concentração inicial do inóculo de 4.106 esporos g-1 e tamanho de partícula de 0,6 mm. O estudo de otimização do processo indicou que os melhores resultados de atividade enzimática para a protease foram obtidos em pH 3,0 e tamanho de partícula dos grãos de soja de 0,6 mm após a fragmentação para as fermentações.Soybean has a wide availability of biochemical compounds as proteins, polysaccharides, lipids, carbohydrates, minerals, vitamins, fibers, lecithin, among others, making interesting its employment as substrate for solid state fermentation. Although the process of SSF is not extensively used industrially like the submerged fermentation (SmF, it can be a viable alternative, since presents higher results of productivity, especially at the cultivation

  9. Pepsin-Cellulase Digestibility of Pasture Silages: Effects of Pasture Type, Maturity Stage, and Variations in the Enzymatic Method Digestibilidad mediante Pepsina-Celulasa de Ensilajes de Pradera: Efectos del Tipo de Pradera, Estado de Madurez y Variaciones en el Método Enzimático

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    Claudia Barchiesi-Ferrari

    2011-06-01

    Full Text Available Enzymatic in vitro digestibility has been studied as a method to predict energy values of forages for ruminants, although results have been affected by type of forage and methodological details of the technique. This work was performed to evaluate the effects of cellulase concentration (0.75, 1.0 and 6.25 g L-1, incubation time (24 or 48 h and type of final washing of the residue (water or acetone on the in vitro digestibility of the dry matter (DMD, organic matter (OMD and content of digestible organic matter in the DM (D value of silages made at three maturity stages from three types of pastures: a permanent pasture (Dactylis glomerata, Lolium perenne, Bromus catharticus Vahl var. catharticus, Trifolium repens and Holcus lanatus; b Italian ryegrass ley (Lolium multiflorum Lam. cv. Tama; c oats (Avena sativa L. and d mixed pasture (L. perenne-T. repens. Regression equations among cellulase results and in vitro values obtained with rumen fluid were also developed. Higher enzyme concentration, longer incubation time and final washing with acetone, resulted in a significant (P La digestibilidad enzimática in vitro ha sido estudiada para predecir el valor energético de forrajes para rumiantes, aunque el tipo de forraje y los detalles metodológicos han afectado los resultados obtenidos. Este trabajo pretende evaluar los efectos de la concentración de celulasa (0,75; 1,0 y 6,25 g L-1, tiempo de incubación (24 o 48 h y tipo de lavado final (agua o acetona del residuo, sobre la digestibilidad in vitro de la materia seca (DMD, materia orgánica (OMD y el contenido de materia orgánica digestible en la materia seca, o valor D (D value de ensilajes hechos con tres estados de madurez, de diferentes praderas: a pradera permanente (Dactylis glomerata, Lolium perenne, Bromus catharticus Vahl var. catharticus, Trifolium repens y Holcus lanatus; b ballica Italiana (Lolium multiflorum Lam. cv. Tama; c avena (Avena sativa L.; y d pradera mixta (L. perenne

  10. Variación estacional y potencial enzimático de microhongos asociados con la descomposición de hojarasca de Nothofagus pumilio Seasonal variation and enzymatic potential of microfungi associated with the decomposition of Nothofagus pumilio leaf litter

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    EDUARDO VALENZUELA

    2001-12-01

    Full Text Available Se estudió la variación estacional y el potencial enzimático de microhongos aislados desde el follaje y hojarasca de Nothofagus pumilio sometida a degradación natural durante 1 año. La investigación se realizó en un bosque de N. pumilio ubicado en una microcuenca del Valle de Antillanca, Parque Nacional Puyehue (40° 47' S, 72° 12' O, 1.120 m de altitud. Hojas senescentes colectadas desde árboles de N. pumilio se depositaron en bolsas de malla de nylon ("litter bags" y se sometieron a degradación natural en el piso del bosque durante 1 año (marzo 1997-marzo 1998, realizando muestreos trimestrales. Para el aislamiento de microhongos desde las hojas se utilizó el método de las diluciones, con agar extracto de malta al 2 % como medio de cultivo. Para determinar los potenciales degradativos de los aislamientos, se analizaron in vitro las actividades de amilasa, celulasa, pectinasa, proteasa, lacasa, oxidasa extracelular, peroxidasa, citocromo oxidasa, fosfatasa, esterasa y tirosinasa. Las principales especies aisladas en las hojas senescentes fueron Alternaria alternata, Cladosporium cladosporioides, Epicoccum nigrum, Phialophora grupo hoffmannii y Rhodotorula aurantiaca. Las especies dominantes en la hojarasca fueron Hormonema prunorum, Mortierella ramanniana var. angulispora, Penicillium para-herquei y Trichoderma polysporum. El mayor potencial enzimático lo exhibieron Moniliales y micelios estériles, mientras el menor Sphaeropsidales y levaduras. Las especies más activas fueron Alternaria alternata, Cladosporium cladosporioides, Hormonema prunorum y Phialophora malorum. Las actividades de celulasa y amilasa fueron las más importantes. De las enzimas ligninolíticas, oxidasa extracelular y peroxidasa presentaron los más elevados potenciales enzimáticosThe seasonal variation and enzymatic potential of microfungi isolated from senescent leaves and leaf litter of Nothofagus pumilio was studied. The study was performed in a N

  11. Designing for Persuasion: Toward Ambient Eco-Visualization for Awareness

    Science.gov (United States)

    Kim, Tanyoung; Hong, Hwajung; Magerko, Brian

    When people are aware of their lifestyle's ecological consequences, they are more likely to adjust their behavior to reduce their impact. Persuasive design that provides feedback to users without interfering with their primary tasks can increases the awareness of neighboring problems. As a case study of design for persuasion, we designed two ambient displays as desktop widgets. Both represent a users' computer usage time, but in different visual styles. In this paper, we present the results of a comparative study of two ambient displays. We discuss the gradual progress of persuasion supported by the ambient displays and the differences in users' perception affected by the different visualization styles. Finally, Our empirical findings lead to a series of design implications for persuasive media.

  12. The persistent signature of tropical cyclones in ambient seismic noise

    KAUST Repository

    Gualtieri, Lucia; Camargo, Suzana J.; Pascale, Salvatore; Pons, Flavio M.E.; Ekströ m, Gö ran

    2017-01-01

    The spectrum of ambient seismic noise shows strong signals associated with tropical cyclones, yet a detailed understanding of these signals and the relationship between them and the storms is currently lacking. Through the analysis of more than a decade of seismic data recorded at several stations located in and adjacent to the northwest Pacific Ocean, here we show that there is a persistent and frequency-dependent signature of tropical cyclones in ambient seismic noise that depends on characteristics of the storm and on the detailed location of the station relative to the storm. An adaptive statistical model shows that the spectral amplitude of ambient seismic noise, and notably of the short-period secondary microseisms, has a strong relationship with tropical cyclone intensity and can be employed to extract information on the tropical cyclones.

  13. Ambient Radon-222 Monitoring in Amargosa Valley, Nevada

    International Nuclear Information System (INIS)

    L.H. Karr; J.J. Tappen; D. Shafer; K.J. Gray

    2008-01-01

    As part of a program to characterize and baseline selected environmental parameters in the region around the proposed repository at Yucca Mountain, Nevada, ambient radon-222 monitoring was conducted in the rural community of Amargosa Valley, the community closest to the proposed repository site. Passive integrating radon monitors and a continuous radon monitoring instrument were deployed adjacent to the Community Environmental Monitoring Program (CEMP) (http://www.cemp.dri.edu/index.html) station located in the Amargosa Valley Community Center near the library. The CEMP station provided real-time ambient gamma exposure and meteorological data used to correct the integrated radon measurements as well as verify meteorological data collected by the continuous radon monitoring instrument. Additionally, different types of environmental enclosures that housed the monitors and instrument were used to determine if particular designs influenced the ambient radon measurements

  14. Superheated emulsions in neutron spectrometry by varying ambient pressure

    International Nuclear Information System (INIS)

    Das, Mala; Sawamura, Teruko

    2005-01-01

    The principle of present work lies on the dependence of the threshold neutron energy on the dimensionless quantity ''degree of metastability (ss)'' of superheated liquids. The response of the superheated emulsions consists of the drops of superheated liquid (C 2 Cl 2 F 4 , b.p. 3.77 deg. C) has been measured at different 'ss' by varying ambient pressure at different temperatures, in the presence of neutrons generated in Pb by a (γ,n) reaction from 45 MeV electron LINAC of Hokkaido University. To unfold the neutron energy spectrum, a relationship has been developed between the 'ss' of superheated liquids and the threshold neutron energy. The spectrum at the detector position has been calculated by the MCNP code and a comparison has been made with the experimental spectrum. The utilisation of 'ss' is more flexible as this relation can be applied to both positive and negative ambient pressures as well as at different ambient temperatures

  15. El compromiso ambiental universitario: un desafio curricular para trascender

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    Yamile Pedraza-Jiménez

    2017-12-01

    Full Text Available El presente artículo desarrolla una revisión documental sobre la relación y discusión del concepto interdisciplinariedad y su aplicación en el campo ambiental. Pretende informar y complementar el conocimiento existente sobre el supuesto teórico de la interdiciplinariedad que demanda la inclusión de la dimensión ambiental en el contexto universitario indicando sus controversias, límites y posibles vías para abordarla como propuesta curricular. Finalmente, se realiza una discusión sobre el trascender de la interdisciplinariedad al diálogo de saberes como una propuesta política desde el contexto de América Latina, para contribuir a proyectar los fines misionales y el compromiso ambiental universitario.

  16. Health Effects of Ambient Air Pollution in Developing Countries.

    Science.gov (United States)

    Mannucci, Pier Mannuccio; Franchini, Massimo

    2017-09-12

    The deleterious effects of ambient air pollution on human health have been consistently documented by many epidemiologic studies worldwide, and it has been calculated that globally at least seven million deaths are annually attributable to the effects of air pollution. The major air pollutants emitted into the atmosphere by a number of natural processes and human activities include nitrogen oxides, volatile organic compounds, and particulate matter. In addition to the poor ambient air quality, there is increasing evidence that indoor air pollution also poses a serious threat to human health, especially in low-income countries that still use biomass fuels as an energy resource. This review summarizes the current knowledge on ambient air pollution in financially deprived populations.

  17. Ambient plasma treatment of silicon wafers for surface passivation recovery

    Science.gov (United States)

    Ge, Jia; Prinz, Markus; Markert, Thomas; Aberle, Armin G.; Mueller, Thomas

    2017-08-01

    In this work, the effect of an ambient plasma treatment powered by compressed dry air on the passivation quality of silicon wafers coated with intrinsic amorphous silicon sub-oxide is investigated. While long-time storage deteriorates the effective lifetime of all samples, a short ambient plasma treatment improves their passivation qualities. By studying the influence of the plasma treatment parameters on the passivation layers, an optimized process condition was identified which even boosted the passivation quality beyond its original value obtained immediately after deposition. On the other hand, the absence of stringent requirement on gas precursors, vacuum condition and longtime processing makes the ambient plasma treatment an excellent candidate to replace conventional thermal annealing in industrial heterojunction solar cell production.

  18. The persistent signature of tropical cyclones in ambient seismic noise

    KAUST Repository

    Gualtieri, Lucia

    2017-12-28

    The spectrum of ambient seismic noise shows strong signals associated with tropical cyclones, yet a detailed understanding of these signals and the relationship between them and the storms is currently lacking. Through the analysis of more than a decade of seismic data recorded at several stations located in and adjacent to the northwest Pacific Ocean, here we show that there is a persistent and frequency-dependent signature of tropical cyclones in ambient seismic noise that depends on characteristics of the storm and on the detailed location of the station relative to the storm. An adaptive statistical model shows that the spectral amplitude of ambient seismic noise, and notably of the short-period secondary microseisms, has a strong relationship with tropical cyclone intensity and can be employed to extract information on the tropical cyclones.

  19. Viabilidade, confirmação taxonômica e detecção enzimática de espécies de Acremonium preservadas sob óleo mineral na Coleção de Culturas University Recife Mycology Viability, taxonomic confirmation and enzymatic detection of Acremonium species preserved under mineral oil in the URM Culture Collection

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    Suellen Carvalho de Moura Braz

    2009-02-01

    Full Text Available Enzimas hidrolíticas secretadas por fungos têm um papel importante na patogenicidade das infecções. Objetivando avaliar a atividade enzimática foram testados 31 isolados de Acremonium mantidos na Coleção de Culturas University Recife Mycology. Fragmentos das culturas foram transferidos para caldo glicosado para reativação e posterior crescimento em meio ágar batata dextrose, para verificar viabilidade, pureza e confirmação taxonômica pela observação das características macroscópicas e microscópicas. Para detecção enzimática foram utilizados substratos de caseína do leite e gelatina para protease, amido para amilase e lecitina de soja para fosfolipase. Das 31 culturas, 26 (83,9% mantiveram-se viáveis e 24 (92,3% foram confirmadas taxonomicamente. Das 24 culturas, 12 (50% apresentaram atividade proteásica, duas (16,7% em caseína do leite, uma (8,3% em gelatina e nove (75% em ambos os substratos; 16 (66,7% degradaram amido. Nenhuma cultura apresentou atividade fosfolipásica. Conclui-se que espécies de Acremonium são capazes de produzir enzimas envolvidas na patogenicidade das infecções fúngicas.Hydrolytic enzymes secreted by fungi play an important role in the pathogenesis of infection. With the aim of evaluating the enzymatic activity, 31 isolates of Acremonium stored in the University of Recife Mycology (URM Culture Collection were tested. Culture fragments were transferred to glycoside broth for reactivation and further growth in potato dextrose agar medium in order to investigate viability and purity and to confirm the taxonomy through observing the macroscopic and microscopic characteristics. To detect enzymes, milk casein and gelatin were used as substrates for proteinase, starch for amylase and soy lecithin for phospholipase. Among the 31 cultures, 26 (83.9% remained viable and 24 (92.3% were confirmed taxonomically. Out of these 24 cultures, 12 (50% presented proteinase activity, of which two (16.7% were on

  20. Influência do tamanho das partículas na solubilização enzimática da proteína do farelo de soja = The influence of particle size in the enzymatic extraction of soybean meal protein

    Directory of Open Access Journals (Sweden)

    Maura Stenzel

    2009-04-01

    Full Text Available O objetivo deste trabalho foi verificar a influência do tamanho das partículas na quantidade de proteína solubilizada a partir do farelo de soja. O farelo foi peneirado e cada fração foi submetida à hidrólise enzimática com as proteases Alcalase e Flavourzyme. Ashidrólises foram realizadas a 60ºC, agitação de 100 rpm, concentração de Alcalase 0,5% (proteína/proteína por 3h e concentração de Flavourzyme 1% (proteína/proteína por 5h. A quantidade de proteína solubilizada foi determinada pelo método de Lowry. A análisegranulométrica do farelo mostrou que quase metade da amostra corresponde a partículas maiores que 1,68 mm. O modelo que resultou em melhor ajuste dos dados foi o GGS, e o diâmetro médio de Sauter da amostra foi 1,150 mm. Com relação à hidrólise enzimática,para as duas enzimas foi verificada uma tendência de aumento na quantidade solubilizada para as frações mais finas e maior efetividade da Alcalase na solubilização proteica. The objective of this work was to verify the influence of particlesize in the results of enzymatic extraction of soybean meal protein. The meal was classified in homogeneous fractions, and each was subjected to enzymatic hydrolysis with Alcalase and Flavourzyme proteases. The hydrolyses were conducted at 60ºC, with agitation of 100 rpm, 0.5% concentration of Alcalase (protein/protein for three hours, and 1% concentration of Flavourzyme (protein/protein for five hours. The results were analyzed based on the amount of solubilized protein, determined through the Lowry method. The granulometric analysis of the meal showed that almost half of the sample is composed of particles larger that 1.68 mm. The model that resulted in the best adjustment of experimental data was GGS, and the Sauter mean diameter of the sample was 1.150 mm. Regarding enzymatic hydrolysis, a trend of increase in the amount of solubilized proteinwas verified for both enzymes for the finer fractions and greater

  1. Determinación enzimática y metales pesados en cerebro e hígado del modelo ecotoxicológico Gambusia punctata (Poeciliidae Enzymatic determination and heavy metals in the brain and liver in ecotoxicological model Gambusia punctata (Poeciliidae

    Directory of Open Access Journals (Sweden)

    George Argota Pérez

    2013-02-01

    Full Text Available Se realizó un estudio durante el 2011, con vistas a evaluar los niveles de actividad enzimática y metales pesados bioacumulados en cerebro e hígado de la especie Gambusia punctata, que habita en los ecosistemas San Juan y Filé de Santiago de Cuba, para lo cual se seleccionaron 3 estaciones, correspondientes a la parte alta, media y baja de ambos sistemas. Se escogieron ejemplares que midieron biométricamente de 2,1-3,0 cm de longitud total. Se determinó la enzima acetilcolinesterasa en el cerebro y la glutation-S-transferasa y catalasa, respectivamente, en el hígado. En ambos órganos se analizaron concentraciones de cobre, cinc, plomo y cadmio, tratados por vía húmeda y cuantificados por espectroscopia de plasma inductivamente acoplado con vista axial. En la especie del San Juan, los niveles enzimáticos variaron entre las estaciones, siendo mayores y estadísticamente diferentes para las hembras (pA study during 2011 was carried out in order to evaluate the levels of enzymatic activity and heavy metals bioaccumulated in brain and liver of the species Gambusia punctata, inhabiting San Juan and Filé ecosystems in Santiago de Cuba. For this purpose 3 stations were selected, corresponding to the upper, middle and lower parts of both systems. Specimens biometrically measuring 2.1-3.0 cm in total length were chosen. Acetylcholinesterase enzyme in the brain and glutathione-S-transferase and catalase in the liver were determined, respectively. Copper, zinc, lead and cadmium concentrations were analyzed in both organs, wet processed and quantified by axial view inductively coupled plasma spectroscopy. In San Juan species the enzymatic levels varied between the stations, being higher and statistically different for females (p<0.05, but in that from Filé they were similar between the parts and genders. Regarding metals, concentrations were not detected in brain, neither lead nor cadmium in the liver. Copper and zinc concentrations were higher

  2. Efeito da hidrólise enzimática sobre propriedades funcionais de caseína bovina coagulada pela ação da quimosina Effect of enzimatic hydrolysis on functional properties of bovine casein coagulated by the action of chymosin

    Directory of Open Access Journals (Sweden)

    Janesca Alban Roman

    2005-09-01

    Full Text Available Este trabalho teve como objetivo verificar a influência do grau de hidrólise sobre as propriedades funcionais hidrofílicas (capacidade de absorção espontânea de água-CAA, capacidade de retenção de água-CRA e solubilidade da proteína-SP e de superfície (capacidade de formação e estabilização de espumas e de emulsões da caseína bovina obtida por coagulação enzimática. Os graus de hidrólise (GH estudados foram: 5,7; 12,8; 20,1 e 35,8%. Observou-se que quanto maior o GH maior a SP e menor a CRA dos hidrolisados. A hidrólise enzimática melhorou a capacidade de formação de espuma, pois o coágulo de caseína (COC não formou espuma nas condições estudadas. A melhor capacidade de formação de espuma foi conseguida com o GH 12,8% e a maior estabilidade com GH 5,7%. Os hidrolisados, com GH 12,8; 20,1 e 35,8% não formaram emulsão. Não houve diferenças significativas entre o GH 5,7% e o COC quanto à formação de emulsão, mas a estabilidade da emulsão do hidrolisado de menor grau de hidrólise foi significativamente superior ao coágulo de caseína.The objective of the present work was to study the influence of degree of hydrolysis (DH on the hydrophilic and surface functional properties of a bovine casein obtained by enzymatic coagulation (COC. The degrees of hydrolysis produced were 5.7, 12.8, 20.1 and 35.8%. It was observed that the higher the DH, the higher the protein solubility and the lower the water retention capacity (WRC of the hydrolysates. The best foaming capacity was obtained with 12.8% DH and best foam stability with 5.7% DH. The hydrolysate with 5.7% DH showed the same emulsifying capacity as the coagulated casein, however emulsion stability was superior for the 5.7% hydrolysate than for COC. The hydrolysates with higher DH (12.8, 20.1 and 35.8% did not form emulsion.

  3. Atividades hidrolíticas de amostras de conteúdo do intestino delgado de bovinos adultos, infectados ou não por Eurytrema sp, determinadas por difusão radial enzimática Hydrolytic activities of small intestine content as determined by radial enzyme diffusion in Eurytrema sp infected or non infected cattle

    Directory of Open Access Journals (Sweden)

    Pacífico Antônio Diniz Belém

    2000-04-01

    Full Text Available Empregando-se difusão radial enzimática em ágar gel, atividades hidrolíticas de amostras de conteúdo do intestino delgado de 38 bovinos adultos de matadouro foram mensuradas em animais agrupados como possuidores de pâncreas hígidos (controle e parasitados por Eurytrema sp, estes, segundo dois graus de infecção reconhecidos. Para tal, foram empregados quatro substratos, dos quais caseinato de cálcio e óleo de milho tiveram de ser desconsiderados porque, no caso deles, não houve formação de halos ou estes apresentaram limites imprecisos. Apesar desses problemas não terem ocorrido quando o substrato era leite em pó desnatado ou amido, as atividades hidrolíticas encontradas foram equivalentes, independentemente da condição de parasitismo e grau de infecção verificados (P>0,05. Concluiu-se, então, que a técnica de difusão radial enzimática, pelo menos com os substratos empregados, foi incapaz de acusar disfunções pancreáticas exócrinas em bovinos infectados por Eurytrema sp.Hidrolitic activities of small intestine content as determined by radial enzyme diffusion in agar gel containing calcium caseinate, corn oil, starch or skimmed milk were measured in 38 mature cattle from slaughterhouse. Each one were identified as normal or infected and then classified into two different grades according to pancreatic ducts lesions and parasite burden. The substrates calcium caseinate and corn oil did not shown cleared zones in several radial enzyme diffusion tests or it was difficult to get a correct measure of the diameters. Such problems, however, were not observed with starch and skimmed milk. In these cases, hidrolitic activities were similar in both control and Eurytrema sp infected groups, despite of the pancreatic duct lesions and parasite load (P>0.05. So, it was concluded that the radial enzyme diffusion method couldn’t shown any exocrine pancreatic disfunction in Eurytrema sp infected cattle.

  4. INFLUÊNCIA DO TREINAMENTO AMBIENTAL NA CONSCIÊNCIA AMBIENTAL DE PROFISSIONAIS DA ÁREA DE RECURSOS HUMANOS

    Directory of Open Access Journals (Sweden)

    Thais Accioly Baccaro

    2015-08-01

    Full Text Available Actualmente, muchas organizaciones trabajan para minimizar y contribuir para resolver los problemasambientales. En esa dirección, el área de recursos humanos emerge como facilitadora de la implantación deprácticas y políticas ambientales en las empresas, principalmente por la utilización de los programas deentrenamiento. Esta investigación tiene como objetivo analizar la influencia del entrenamiento ambiental enla conciencia ambiental de los profesionales de recursos humanos. Con un abordaje cuantitativodescriptivo, se aplicó un cuestionario, ya validado, a 73 profesionales de recursos humanos de la región deLondrina-PR. El análisis de datos involucró la estadística descriptiva y el teste sin parámetros χ 2 dePearson. Los resultados señalaron la relación entre el entrenamiento ambiental y la conciencia ambientalde los profesionales de recursos humanos investigados: de hecho, los que recibieron algún tipo deentrenamiento ambiental presentaron un nivel de conciencia ambiental mayor que los que no recibieronningún entrenamiento en esa área.

  5. Visual attention for a desktop virtual environment with ambient scent

    Directory of Open Access Journals (Sweden)

    Alexander eToet

    2013-11-01

    Full Text Available In the current study participants explored a desktop virtual environment (VE representing a suburban neighborhood with signs of public disorder (neglect, vandalism and crime, while being exposed to either room air (control group, or subliminal levels of tar (unpleasant; typically associated with burned or waste material or freshly cut grass (pleasant; typically associated with natural or fresh material ambient odor. They reported all signs of disorder they noticed during their walk together with their associated emotional response. Based on recent evidence that odors reflexively direct visual attention to (either semantically or affectively congruent visual objects, we hypothesized that participants would notice more signs of disorder in the presence of ambient tar odor (since this odor may bias attention to unpleasant and negative features, and less signs of disorder in the presence of ambient grass odor (since this odor may bias visual attention towards the vegetation in the environment and away from the signs of disorder. Contrary to our expectations the results show that the presence of an ambient odor did not affect the participants’ visual attention for signs of disorder or their emotional response. We conclude that a closer affective, semantic or spatiotemporal link between the contents of a desktop VE and ambient scents may be required to effectively establish diagnostic associations that guide a user’s attention. In the absence of these direct links, ambient scent may be more diagnostic for the physical environment of the observer as a whole than for the particular items in that environment (or, in this case, items represented in the VE.

  6. Visual attention for a desktop virtual environment with ambient scent.

    Science.gov (United States)

    Toet, Alexander; van Schaik, Martin G

    2013-01-01

    In the current study participants explored a desktop virtual environment (VE) representing a suburban neighborhood with signs of public disorder (neglect, vandalism, and crime), while being exposed to either room air (control group), or subliminal levels of tar (unpleasant; typically associated with burned or waste material) or freshly cut grass (pleasant; typically associated with natural or fresh material) ambient odor. They reported all signs of disorder they noticed during their walk together with their associated emotional response. Based on recent evidence that odors reflexively direct visual attention to (either semantically or affectively) congruent visual objects, we hypothesized that participants would notice more signs of disorder in the presence of ambient tar odor (since this odor may bias attention to unpleasant and negative features), and less signs of disorder in the presence of ambient grass odor (since this odor may bias visual attention toward the vegetation in the environment and away from the signs of disorder). Contrary to our expectations the results provide no indication that the presence of an ambient odor affected the participants' visual attention for signs of disorder or their emotional response. However, the paradigm used in present study does not allow us to draw any conclusions in this respect. We conclude that a closer affective, semantic, or spatiotemporal link between the contents of a desktop VE and ambient scents may be required to effectively establish diagnostic associations that guide a user's attention. In the absence of these direct links, ambient scent may be more diagnostic for the physical environment of the observer as a whole than for the particular items in that environment (or, in this case, items represented in the VE).

  7. Investigation of Transmission Warming Technologies at Various Ambient Conditions

    Energy Technology Data Exchange (ETDEWEB)

    Jehlik, Forrest; Iliev, Simeon; Wood, Eric; Gonder, Jeff

    2017-03-28

    This work details two approaches for evaluating transmission warming technology: experimental dynamometer testing and development of a simplified transmission efficiency model to quantify effects under varied real world ambient and driving conditions. Two vehicles were used for this investigation: a 2013 Ford Taurus and a 2011 Ford Fusion. The Taurus included a production transmission warming system and was tested over hot and cold ambient temperatures with the transmission warming system enabled and disabled. A robot driver was used to minimize driver variability and increase repeatability. Additionally the Fusion was tested cold and with the transmission pre-heated prior to completing the test cycles. These data were used to develop a simplified thermally responsive transmission model to estimate effects of transmission warming in real world conditions. For the Taurus, the fuel consumption variability within one standard deviation was shown to be under 0.5% for eight repeat Urban Dynamometer Driving Cycles (UDDS). These results were valid with the transmission warming system active or passive. Using the transmission warming system under 22 degrees C ambient temperature, fuel consumption reduction was shown to be 1.4%. For the Fusion, pre-warming the transmission reduced fuel consumption 2.5% for an urban drive cycle at -7 degrees C ambient temperature, with 1.5% of the 2.5% gain associated with the transmission, while consumption for the US06 test was shown to be reduced by 7% with 5.5% of the 7% gain associated with the transmission. It was found that engine warming due to conduction between the pre-heated transmission and the engine resulted in the remainder of the benefit. For +22 degrees C ambient tests, the pre-heated transmission was shown to reduce fuel consumption approximately 1% on an urban cycle, while no benefit was seen for the US06 cycle. The simplified modeling results showed gains in efficiency ranging from 0-1.5% depending on the ambient

  8. Ambient temperature affects postnatal litter size reduction in golden hamsters.

    Science.gov (United States)

    Ohrnberger, Sarah A; Monclús, Raquel; Rödel, Heiko G; Valencak, Teresa G

    2016-01-01

    To better understand how different ambient temperatures during lactation affect survival of young, we studied patterns of losses of pups in golden hamsters ( Mesocricetus auratus ) at different ambient temperatures in the laboratory, mimicking temperature conditions in natural habitats. Golden hamsters produce large litters of more than 10 young but are also known to wean fewer pups at the end of lactation than they give birth to. We wanted to know whether temperature affects litter size reductions and whether the underlying causes of pup loss were related to maternal food (gross energy) intake and reproductive performance, such as litter growth. For that, we exposed lactating females to three different ambient temperatures and investigated associations with losses of offspring between birth and weaning. Overall, around one third of pups per litter disappeared, obviously consumed by the mother. Such litter size reductions were greatest at 30 °C, in particular during the intermediate postnatal period around peak lactation. Furthermore, litter size reductions were generally higher in larger litters. Maternal gross energy intake was highest at 5 °C suggesting that mothers were not limited by milk production and might have been able to raise a higher number of pups until weaning. This was further supported by the fact that the daily increases in litter mass as well as in the individual pup body masses, a proxy of mother's lactational performance, were lower at higher ambient temperatures. We suggest that ambient temperatures around the thermoneutral zone and beyond are preventing golden hamster females from producing milk at sufficient rates. Around two thirds of the pups per litter disappeared at high temperature conditions, and their early growth rates were significantly lower than at lower ambient temperatures. It is possible that these losses are due to an intrinsic physiological limitation (imposed by heat dissipation) compromising maternal energy intake and

  9. Ambient Seismic Imaging of Hydraulically Active Fractures at km Depths

    Science.gov (United States)

    Malin, P. E.; Sicking, C.

    2017-12-01

    Streaming Depth Images of ambient seismic signals using numerous, densely-distributed, receivers have revealed their connection to hydraulically active fractures at 0.5 to 5 km depths. Key for this type of imaging is very high-fold stacking over both multiple receives and periods of a few hours. Also important is suppression of waveforms from fixed, repeating sources such as pumps, generators, and traffic. A typical surface-based ambient SDI survey would use a 3D seismic receiver grid. It would have 1,000 to 4,000 uniformly distributed receivers at a density of 50/km2over the target. If acquired by borehole receivers buried 100 m deep, the density can be dropped by an order of magnitude. We show examples of the acquisition and signal processing scenarios used to produce the ambient images. (Sicking et al., SEG Interpretation, Nov 2017.) While the fracture-fluid source connection of SDI has been verified by drilling and various types of hydraulic tests, the precise nature of the signal's origin is not clear. At the current level of observation, the signals do not have identifiable phases, but can be focused using P wave velocities. Suggested sources are resonances of pressures fluctuations in the fractures, or small, continuous, slips on fractures surfaces. In either case, it appears that the driving mechanism is tectonic strain in an inherently unstable crust. Solid earth tides may enhance these strains. We illustrate the value of the ambient SDI method in its industrial application by showing case histories from energy industry and carbon-capture-sequestration projects. These include ambient images taken before, during, and after hydraulic treatments in un-conventional reservoirs. The results show not only locations of active fractures, but also their time responses to stimulation and production. Time-lapse ambient imaging can forecast and track events such as well interferences and production changes that can result from nearby treatments.

  10. Passive radiative cooling below ambient air temperature under direct sunlight.

    Science.gov (United States)

    Raman, Aaswath P; Anoma, Marc Abou; Zhu, Linxiao; Rephaeli, Eden; Fan, Shanhui

    2014-11-27

    Cooling is a significant end-use of energy globally and a major driver of peak electricity demand. Air conditioning, for example, accounts for nearly fifteen per cent of the primary energy used by buildings in the United States. A passive cooling strategy that cools without any electricity input could therefore have a significant impact on global energy consumption. To achieve cooling one needs to be able to reach and maintain a temperature below that of the ambient air. At night, passive cooling below ambient air temperature has been demonstrated using a technique known as radiative cooling, in which a device exposed to the sky is used to radiate heat to outer space through a transparency window in the atmosphere between 8 and 13 micrometres. Peak cooling demand, however, occurs during the daytime. Daytime radiative cooling to a temperature below ambient of a surface under direct sunlight has not been achieved because sky access during the day results in heating of the radiative cooler by the Sun. Here, we experimentally demonstrate radiative cooling to nearly 5 degrees Celsius below the ambient air temperature under direct sunlight. Using a thermal photonic approach, we introduce an integrated photonic solar reflector and thermal emitter consisting of seven layers of HfO2 and SiO2 that reflects 97 per cent of incident sunlight while emitting strongly and selectively in the atmospheric transparency window. When exposed to direct sunlight exceeding 850 watts per square metre on a rooftop, the photonic radiative cooler cools to 4.9 degrees Celsius below ambient air temperature, and has a cooling power of 40.1 watts per square metre at ambient air temperature. These results demonstrate that a tailored, photonic approach can fundamentally enable new technological possibilities for energy efficiency. Further, the cold darkness of the Universe can be used as a renewable thermodynamic resource, even during the hottest hours of the day.

  11. EPISTEMOLOGIA AMBIENTAL: A crise ambiental como uma crise da razão

    Directory of Open Access Journals (Sweden)

    Matheus Silva De Gregori

    2013-04-01

    Full Text Available O presente estudo tem como objetivo analisar a crise do meio ambiente sob uma abordagem epistemológica, ou seja, a partir do conhecimento produzido em relação à natureza. Verifica-se que o pensamento ocidental, manifestado pelos paradigmas da Ciência Moderna, está ligado à organização dos sistemas econômicos que reconheceram historicamente a natureza apenas como recurso e potencial de produção de capital, vindo a encontrar, por esta via, o limite entrópico da biosfera e, por conseguinte, do crescimento. Abordou-se o processo de construção do conhecimento, questionando-se a possibilidade de concepção de outras perspectivas, fundadas na inseparabilidade entre sujeito-objeto, ação-pensamento e homem-natureza. É percebida a necessidade do ser humano de se re-significar no mundo, conhecendo a complexidade da natureza e pensando além da crise ecológica.

  12. Los sinuosos caminos del racismo: el racismo ambiental en Argentina

    OpenAIRE

    Rodríguez Mir, Javier

    2012-01-01

    El artículo hace referencia a la evolución de las doctrinas racistas y su impacto en las ciencias sociales. Se analiza la capacidad de las doctrinas y políticas racistas de adaptarse a los nuevos contextos. En este sentido, se entiende que el racismo ambiental es una continuación y transformación del racismo que no puede ser desvinculado del contexto histórico, social y político. El autor analiza los orígenes del racismo ambiental en Estados Unidos. Finalmente el artículo reflexiona sobre un ...

  13. Applications of ambient mass spectrometry in high-throughput screening.

    Science.gov (United States)

    Li, Li-Ping; Feng, Bao-Sheng; Yang, Jian-Wang; Chang, Cui-Lan; Bai, Yu; Liu, Hu-Wei

    2013-06-07

    The development of rapid screening and identification techniques is of great importance for drug discovery, doping control, forensic identification, food safety and quality control. Ambient mass spectrometry (AMS) allows rapid and direct analysis of various samples in open air with little sample preparation. Recently, its applications in high-throughput screening have been in rapid progress. During the past decade, various ambient ionization techniques have been developed and applied in high-throughput screening. This review discusses typical applications of AMS, including DESI (desorption electrospray ionization), DART (direct analysis in real time), EESI (extractive electrospray ionization), etc., in high-throughput screening (HTS).

  14. Characterization of CdZnTe ambient temperature detectors

    International Nuclear Information System (INIS)

    Lavietes, A.

    1994-09-01

    A great deal of interest has been generated in the use of cadmium telluride (CdTe) and cadmium zinc telluride (CdZnTe) detectors for ambient temperature detection of radionuclides. The addition of zinc to CdTe provides several benefits that enhance the materials operational characteristics at ambient temperature. Recent movement in the industry is to produce larger volume detectors using CdZnTe without much known about the effects of larger geometry on performance. The purpose of this study is to get an idea of the relationship of detector performance to both area and thickness variations

  15. Comparison of System Identification Methods using Ambient Bridge Test Data

    DEFF Research Database (Denmark)

    Andersen, P.; Brincker, Rune; Peeters, B.

    1999-01-01

    In this paper the performance of four different system identification methods is compared using operational data obtained from an ambient vibration test of the Swiss Z24 highway bridge. The four methods are the frequency domain based peak-picking methods, the polyreference LSCE method, the stocha......In this paper the performance of four different system identification methods is compared using operational data obtained from an ambient vibration test of the Swiss Z24 highway bridge. The four methods are the frequency domain based peak-picking methods, the polyreference LSCE method...

  16. Medio ambiente y desarrollo: su implicancia nacional e internacional

    OpenAIRE

    Martínez Lestard, Pablo

    1992-01-01

    Cuando el Presidente de la Comisión Mundial sobre el Medio Ambiente y el Desarrollo, el Sr. Gro Harlem Brundtland, Primer Ministro de Noruega, presentó el reporte "Nuestro Futuro Común" a la Asamblea General de la ONU en 1987, fue el momento en que se dio a conocer, ante el foro mundial, la idea del "desarrollo sostenible". Según Lloyd Timberlake, editor principal en el Instituto Internacional para el Medio Ambiente y el Desarrollo (Londres), el reporte "extremista" únicamente en cu...

  17. La contaminación ambiental en el derecho alimentario

    OpenAIRE

    Mondéjar Pedreño, Remedios

    2016-01-01

    El contenido de esta Tesis Doctoral es un análisis de la interrelación entre el Derecho Alimentario europeo y el Derecho ambiental, y en particular del Derecho del medio ambiente, en la legislación alimentaria europea. Para ello nuestro estudio se centra en el Reglamento (CE) 178/2002, del Parlamento Europeo y del Consejo, de 28 de enero de 2002, que es el pilar fundamental del Derecho Alimentario de la Unión Europea. En su extenso título, esta disposición informa sobre el triple contenido de...

  18. Desastre ecológico y ambiental en Huancavelica

    OpenAIRE

    Pinto Herrera, Honorio

    2014-01-01

    El trabajo trata de confirmar, una vez más, que la actividad minera en el Perú afecta los sistemas ecológicos y el medio ambiente. Esto es posible debido al poder de las empresas y a la debilidad y complicidad del Estado. En este sentido, uno de los objetivos de esta investigación es contribuir al conocimiento de un problema nacional, que es la contaminación ambiental a partir de un caso concreto: el derrame de relaves en la mina Caudalosa Chica. Los temas centrales desarrollados abarcan l...

  19. EDUCAÇÃO AMBIENTAL E A SUSTENTABILIDADE DO CERRADO

    OpenAIRE

    Freire, Arnaldo Cardoso; FACULDADE ARAGUAIA

    2013-01-01

    O presente artigo traz uma reflexão sobre a Educação Ambiental e sua contribuição para asustentabilidade do bioma cerrado, discutindo seu conceito, sua evolução, seus objetivos, etemas de estudo. Buscando compreender como se da o surgimento de uma consciênciaambiental e se ela pode ser estabelecida a partir de uma disciplina específica de meioambiente ou por qualquer forma de ensino que possa ser inserida. E de que forma a EducaçãoAmbiental contribui par a redução das agressões constantes sof...

  20. Impactos del turismo en el medio ambiente de huanchaco

    OpenAIRE

    Zavaleta Alfaro, María Sixtina

    2007-01-01

    Se analiza los impactos negativos del turismo sobre el medio ambiente de la Bahía de Huanchaco, previamente se evalúa el flujo de visitantes, la oferta turística en cuanto a recursos naturales, culturales y planta o infraestructura (hoteles, restaurantes, otros). Se incide en al generación de residuos sólidos domésticos, calidad del agua, transporte, deterioro de ecosistema de humedales (calidad ambiental) y se analiza la opinión o percepción del turista a través de una encuesta. Los resulta...

  1. El estado actual de los ambientes del agua

    OpenAIRE

    Mateu Bellés, Joan F.; Camarasa Belmonte, Ana María

    2008-01-01

    Los ambientes del agua –recursos singulares por sus funciones en la naturaleza y por sus prestaciones y connotaciones para los grupos humanos- son un valioso patrimonio. En concreto, los lagos, los ríos y las masas marinas y subterráneas constituyen bienes territoriales que aportan diversidad ecológica, estructuran paisajes y, a menudo, son utilizados por los humanos para el desarrollo de sus actividades (Martín Montalvo, 1996). A lo largo del tiempo, los ambientes del agua no son estables e...

  2. The DFKI Competence Center for Ambient Assisted Living

    Science.gov (United States)

    Frey, Jochen; Stahl, Christoph; Röfer, Thomas; Krieg-Brückner, Bernd; Alexandersson, Jan

    The DFKI Competence Center for Ambient Assisted Living (CCAAL) is a cross-project and cross-department virtual organization within the German Research Center for Artificial Intelligence coordinating and conducting research and development in the area of Ambient Assisted Living (AAL). Our demonstrators range from multimodal speech dialog systems to fully instrumented environments allowing the development of intelligent assistant systems, for instance an autonomous wheelchair, or the recognition and processing of everyday activities in a smart home. These innovative technologies are then tested, evaluated and demonstrated in DFKI's living labs.

  3. Formation of actinide hexafluorides at ambient temperatures with krypton difluoride

    International Nuclear Information System (INIS)

    Asprey, L.B.; Eller, P.G.; Kinkead, S.A.

    1986-01-01

    A second low-temperature agent, krypton difluoride, for generating volatile plutonium hexafluoride is reported (dioxygen difluoride is the only other reported agent). Plutonium hexafluoride is formed at ambient or lower temperature by the treatment of various solid substrates with krypton difluoride. Volatilization of uranium and neptunium from solid substrates using gaseous krypton difluoride is also reported for the first time. The formation of actinide hexafluorides has been confirmed for the reaction of krypton difluoride in anhydrous HF with UO 2 and with uranium and neptunium fluorides at ambient temperatures. Treatment of americium dioxide with krypton difluoride did not yield americium hexafluoride under the conditions studied. 15 references, 2 figures

  4. Entre el desarrollo y el ambiente: incertidumbres de los agrocombustibles

    OpenAIRE

    León Sicard, Tomás Enrique

    2008-01-01

    El artículo plantea una discusión sobre la manera en que la dimensión ambiental puede ser concebida en relación con el actual concepto de desarrollo, a partir de una visión que entiende lo ambiental como relaciones complejas entre los ecosistemas y las culturas humanas y a partir de este punto, examina el modelo agrario dominante en Colombia versus los modelos de agricultura alternativa y la aparición de los biocombustibles, como una consecuencia ineludible del modelo domi...

  5. Ambient Response Analysis Modal Analysis for Large Structures

    DEFF Research Database (Denmark)

    Brincker, Rune; Andersen, Palle

    1999-01-01

    In this paper an outline is given of the basic ideas in ambient respons analysis, i.e. when modal analyses is performed on systems based on output only. Some of the most known techniques are briefly introduced, and the basic problems discussed. The introduced techniques are the frequency domain...... based peak-picking methods, the polyreference LSCE method, the stochastic subspace method for estimation of state space systems and the prediction error method for estimation of Auto-Regressive Moving Average Vector models. The techniques are illustrated on an example of ambient response measurments...

  6. Revista educomunicativa que promueva la conservación ambiental

    OpenAIRE

    Bermúdez Galarza, Pamela Lucía

    2008-01-01

    En estos últimos años, hemos podido observar que la contaminación de los ríos, aire y suelo es cada vez mayor, pues el ser humano ha ido destruyendo la naturaleza; así tenemos: el botar desperdicios en las calles, quebradas, etc., con lo que ha demostrado su falta de cuidado y educación ambiental; provocando de esta manera efectos nocivos para el medio ambiente como los incendios forestales, la tala indiscriminada de árboles, entre otros. Y es que con la acción de cada individuo, se está prod...

  7. Atlas de Riesgo Ambiental Aplicado en Marinas del Espacio SUDOE

    OpenAIRE

    Avendaño Castro, Pablo A.

    2016-01-01

    RESUMEN: Se analizan 418 marinas del sudoeste europeo utilizando una metodología de evaluación de riesgo ambiental diseñada en el IH Cantabria por Gómez et al. (2016). La zona de estudio es la zona costera del área elegible SUDOE y la región de la Macaronesia, comprendiendo las naciones de Francia (sudoeste), España, Portugal y Reino Unido (Gibraltar). Se obtiene el valor del riesgo ambiental a partir de la estimación de las consecuencias (factores de navegación, dragado y entorno), vulnerabi...

  8. Efeitos de diferentes esforços de luta de judô na atividade enzimática, atividade elétrica muscular e parâmetros biomecânicos de atletas de elite Los efectos de diferentes esfuerzos de lucha del judo en la actividad enzimática, actividad eléctrica muscular y biomecánica de los parámetros de atletas de élite Effects of different strengths in the judo fights, muscular electrical activity and biomechanical parameters in elite athletes

    Directory of Open Access Journals (Sweden)

    Silvia Regina Ribeiro

    2006-02-01

    Full Text Available O treinamento esportivo provoca adaptações neuromusculares e alterações metabólicas visando a performance durante a competição. Nas competições de judô, o número de lutas a que os atletas são submetidos e suas respectivas durações e intervalos são aleatórios, fatores que podem influenciar a performance objetivada no treinamento. O presente estudo investigou a hipótese de que diferentes durações de lutas, 90s, 180s e 300s, poderiam influenciar a atividade enzimática, elétrica muscular e a produção do pico de torque. Antes e após cada luta, foram coletadas amostras sanguíneas dos atletas; em seguida, os mesmos realizaram cinco contrações dinâmicas (90º/s com a utilização de um dinamômetro isocinético (Biodex System 3. Simultaneamente registrou-se o sinal eletromiográfico dos músculos agonista, antagonista e sinergista do movimento avaliado. Não se verificou alteração no torque. As enzimas AST e ALT apresentaram aumento na atividade, nas lutas de 90s (p = 0,0033/p = 0,00059, 180s (p = 0,0044/p = 0,0033 e 300s (p = 0,0044/p = 0,0033. Aumento (p = 0,0180 da atividade da CK após a luta de 300s foi verificado. A LDH diminuiu após a luta de 90s (p = 0,0392. Na análise intermuscular observou-se após a luta de 90s aumento do sinal eletromiográfico do agonista (p = 0,005; na luta de 180s, aumento do antagonista (p = 0,0129 e na luta de 300s, diminuição (p = 0,0137 da atividade do músculo agonista. Observou-se que os esforços da luta de 300s podem ter induzido lesões no tecido muscular caracterizadas pela elevação da CK plasmática, embora a lesão não tenha sido suficiente para detectar fadiga através da dinamometria isocinética. Conclui-se que o protocolo proposto foi suficiente para alteração enzimática e eletromiográfica, sugerindo adaptações metabólicas e neurais a partir do estresse das lutas de judô.El entrenamiento deportivo provoca adaptaciones neuromusculares y alteraciones metab

  9. VIOLÊNCIA E MEIO AMBIENTE: PODE A EDUCAÇÃO AMBIENTAL CONTRIBUIR PARA A PAZ E A SUSTENTABILIDADE?

    Directory of Open Access Journals (Sweden)

    Gustavo Ferreira da Costa Lima

    2010-03-01

    Full Text Available Historicamente a relação social e humana com o meio ambiente sempre implicou em algum tipo de destruição e degradação. Com a constituição da sociedade industrial capitalista e seu desdobramento em sociedade de consumo de massa, esse avanço sobre os recursos naturais tem se dado de forma tão radical que ameaça a continuidade da vida e da biosfera como a conhecemos nos últimos milênios. O texto reflete sobre a relação entre a violência social e o meio ambiente a partir de um feixe de teorias e conceitos provenientes do cruzamento da questão ambiental, da teoria crítica, da teoria da complexidade, da teoria da sociedade de risco e de contribuições de filosofias tradicionais como o Budismo. Os conceitos considerados são os de sustentabilidade, de interdependência, de cidadania ambiental e de justiça socioambiental. Nesse sentido, procura investigar as raízes sociais e psicossociais da violência que condicionam as relações e instituições sócio-culturais, entre as quais figuram a própria ciência, como saber social hegemônico, e a educação; observar suas implicações sobre a vida social e ambiental; e verificar a possível contribuição da educação, em geral, e da educação ambiental como foco curricular, em particular, para transformar ou reorientar essas forças e práticas associadas à violência para fins mais pacíficos e construtivos.

  10. Ambiente y salud: Reflexiones sobre la interacción entre el Derecho Ambiental y el Derecho de la Salud

    OpenAIRE

    Gastón Casaux

    2014-01-01

    Desde el punto de vista sanitario se entiende por Ambiente “el conjunto de condiciones físicas, químicas biológicas, psicológicas, sociales, culturales y económicas en las que vive una población y sus interacciones”.Asu vez, la Salud Pública ha identificado al ambiente como un determinante de la salud, concibiéndolo como el de mayor significación del proceso salud/enfermedad.Por ende, puede llegar a constituirse en un entorno peligroso o saludable.La salud admite entonces cuatro influencias d...

  11. Electric scooters : Batteries in the battle against ambient air pollution?

    NARCIS (Netherlands)

    van Boven, Job FM; An, Pham Le; Kirenga, Bruce J; Chavannes, Niels H.

    2017-01-01

    Ambient air pollution is a major global health threat, responsible for an estimated loss of 103 million disability-adjusted life-years in 2015,1,2 and a main contributor to numerous health problems, such as cardiovascular and respiratory diseases.3,4 Within the traffic domain of air pollution, cars,

  12. A Survey on Ambient Intelligence in Health Care

    Science.gov (United States)

    Acampora, Giovanni; Cook, Diane J.; Rashidi, Parisa; Vasilakos, Athanasios V.

    2013-01-01

    Ambient Intelligence (AmI) is a new paradigm in information technology aimed at empowering people’s capabilities by the means of digital environments that are sensitive, adaptive, and responsive to human needs, habits, gestures, and emotions. This futuristic vision of daily environment will enable innovative human-machine interactions characterized by pervasive, unobtrusive and anticipatory communications. Such innovative interaction paradigms make ambient intelligence technology a suitable candidate for developing various real life solutions, including in the health care domain. This survey will discuss the emergence of ambient intelligence (AmI) techniques in the health care domain, in order to provide the research community with the necessary background. We will examine the infrastructure and technology required for achieving the vision of ambient intelligence, such as smart environments and wearable medical devices. We will summarize of the state of the art artificial intelligence methodologies used for developing AmI system in the health care domain, including various learning techniques (for learning from user interaction), reasoning techniques (for reasoning about users’ goals and intensions) and planning techniques (for planning activities and interactions). We will also discuss how AmI technology might support people affected by various physical or mental disabilities or chronic disease. Finally, we will point to some of the successful case studies in the area and we will look at the current and future challenges to draw upon the possible future research paths. PMID:24431472

  13. Fundamental study of a one-step ambient temperature ferrite ...

    African Journals Online (AJOL)

    ... involves the controlled oxidation of ferrous-containing AMD water at ambient ... solutions under controlled pH and oxidation conditions (pH 10.5, air flow rate ... The kinetics of the reaction under the investigated conditions were found to be ...

  14. Los plaguicidas y la contaminacion del medio ambiente Venezolano

    Science.gov (United States)

    Stickel, L.F.; Stickel, W.H.

    1972-01-01

    RESUMEN DE RECOMENDACIONES Recomendaciones para el Programa de Investigacion: 1. Establecer un sistema de muestreo biologico para detectar los niveles tendencias de los productos quimicos toxicos en un peque?o numero de si tios representativos. 2. Mantener continua vigilancia de la contaminacion ambiental, mediante la seleccion acertadamente dirigida de las zonas afectadas y de las fuentes de contaminacion. 3. Realizar estudios acerca de las poblaciones de animales silvestres, y del exito de los procesos reproductivos de las especies o grupos clayes de animales que se consideran mas gravemente afectados. 4. Preparar recomendaciones para una accion gubernamental de proteccion al hombre, a la fauna silvestre y al medio ambiente. Recomendaciones para la Accion Administrativa: 1. Establecer limites a la tolerancia de los residuos de plaguicidas en los alimentos. Constituye una medida clave para disminuir la contaminacion ambiental. 2. Establecer normas de calidad del agua para las corrientes, represas, la gos y otros cuerpos. Es la segunda medida clave para reducir la contaminacion del ambiente 3. Exigir un tratamiento adecuado de los efluentes industriales, especialmente antes de que se construyan las nuevas plantas. 4. Exigir a los agricultores que en el uso de plaguicidas sigan los consejos tecnicos autorizados y negar a los vendedores el derecho a recomendar productos por su cuenta. 5. Tomar medidas para recoger y eliminar los recipientes y sobrantes de los plaguicidas.

  15. Adaptive Ambient Illumination Based on Color Harmony Model

    Science.gov (United States)

    Kikuchi, Ayano; Hirai, Keita; Nakaguchi, Toshiya; Tsumura, Norimichi; Miyake, Yoichi

    We investigated the relationship between ambient illumination and psychological effect by applying a modified color harmony model. We verified the proposed model by analyzing correlation between psychological value and modified color harmony score. Experimental results showed the possibility to obtain the best color for illumination using this model.

  16. Effects of ambient lighting displays on peripheral activity awareness

    NARCIS (Netherlands)

    Davis, K.; Owusu, E.B.; Marcenaro, L.; Feijs, L.; Regazzoni, C.; Hu, J.

    2017-01-01

    The emergence of ubiquitous sensing and the Internet of Things (IoT) have inspired the development of “smart” everyday objects, which offer tremendous opportunities for maintaining the quality of life in ambient assisted living (AAL) environments. Inspired by the future possibilities of connected

  17. Common Ambient Assisted Living Home Platform for Seamless Care

    DEFF Research Database (Denmark)

    Wagner, Stefan Rahr; Stenner, Rene; Memon, Mukhtiar

    The CareStore project is investigating the feasibility of creating an open and flexible infrastructure for facilitating seamless deployment of assisted living devices and applications on heterogeneous platforms. The Common Ambient Assisted Living Home Platform (CAALHP) is intended to be the main ...

  18. An integrated smart system for ambient-assisted living

    CSIR Research Space (South Africa)

    Foko, Thato E

    2013-08-01

    Full Text Available Ambient-assisted living (AAL) is an initiative to extend the time the elderly can live in their home environment by increasing their autonomy and assisting them carry out their daily activities. AAL systems exploit information and communication...

  19. Distributing emotional services in ambient intelligence through cognitive agents

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.; Vitiello, A.

    2011-01-01

    Ambient Intelligence (AmI) is a pervasive computing paradigm whose main aim is to design smart environments composed of invisible, connected, intelligent and interactive systems, which are naturally sensitive and responsive to the presence of people, providing advanced services for improving the

  20. Evidence of Non-extensivity in Earth's Ambient Noise

    Science.gov (United States)

    Koutalonis, Ioannis; Vallianatos, Filippos

    2017-12-01

    The study of ambient seismic noise is one of the important scientific and practical research challenges, due to its use in a number of geophysical applications. In this work, we describe Earth's ambient noise fluctuations in terms of non-extensive statistical physics. We found that Earth's ambient noise increments follow the q-Gaussian distribution. This indicates that Earth's ambient noise's fluctuations are not random and present long-term memory effects that could be described in terms of Tsallis entropy. Our results suggest that q values depend on the time length used and that the non-extensive parameter, q, converges to value q → 1 for short-time windows and a saturation value of q ≈ 1.33 for longer ones. The results are discussed from the point of view of superstatistics introduced by Beck [Contin Mech Thermodyn 16(3):293-304, 2004] and connects the q values with the system's degrees of freedom. Our work indicates that the converged (maximum) value is q = 1.33 and is related to 5 degrees of freedom.

  1. Modal Identification from Ambient Responses using Frequency Domain Decomposition

    DEFF Research Database (Denmark)

    Brincker, Rune; Zhang, L.; Andersen, P.

    2000-01-01

    In this paper a new frequency domain technique is introduced for the modal identification from ambient responses, ie. in the case where the modal parameters must be estimated without knowing the input exciting the system. By its user friendliness the technique is closely related to the classical ...

  2. Ambient air quality observations in the Athabasca oil sands region

    International Nuclear Information System (INIS)

    1996-01-01

    Both Syncrude and Suncor have plans to develop new oil sands leases and to increase crude oil and bitumen recovery in the Athabasca oil sands region. In recognition of the effects that this will have on the environment, Suncor has proposed modifications to reduce SO 2 emissions to the atmosphere, while Syncrude plans to develop additional ambient air quality, sulphur deposition and biomonitoring programs. This report discussed the ambient air quality monitoring that was undertaken in the Fort McMurray-Fort McKay airshed. Twelve continuous ambient air quality stations and 76 passive monitoring stations are maintained in the region. Environment Canada maintains eight precipitation monitoring stations in northern Alberta and Saskatchewan. Source characterization, ambient air quality and meteorology observations, air quality monitoring, and air quality data from continuous sulphur dioxide, hydrogen sulphide, nitrogen oxides, ozone, carbon monoxide, hydrocarbon, acid rain and particulates analyzers were reviewed. The documentation of all computer files used for the analysis of the air quality data is discussed in the Appendix. 47 refs., 39 tabs., 53 figs

  3. Periodismo ambiental en Canal Sur Televisión

    Directory of Open Access Journals (Sweden)

    Lic. Miguel Montaño Montaño

    1999-01-01

    Full Text Available El autor estudia el origen histórico del periodismo ambiental, la evolución en España y Andalucía y su aparición en Canal Sur TV Realiza un análisis sobre la información medioambiental, especialidad del periodismo que en la década de los noventa despierta nuevamente gran interés entre el público. La televisión autonómica ha dedicado, desde su creación numerosos programas sobre el medio ambiente. Actualmente sus Servicios Informativos tienen la sección Medio Ambiente. De la importancia que se concede a este tipo de información es un buen ejemplo el programa «Tierra y Mar», que incorpora progresivamente noticias medioambientales, referidas a la educación ambiental, el mantenimiento y recuperación de la biodiversidad y la presentación de modelos de conducta respetuosos.

  4. ORGANOCHLORINE PESTICIDES IN THE AMBIENT AIR OF MEXICO

    Science.gov (United States)

    Recent and past use of organochlorine pesticides (OCPs) in Mexico has resulted in concentrations in ambient air that are 1-2 orders of magnitude above levels in the Great Lakes region. Atmospheric transport from Mexico and Central America may be contributing significant amounts ...

  5. A Survey on Ambient Intelligence in Health Care.

    Science.gov (United States)

    Acampora, Giovanni; Cook, Diane J; Rashidi, Parisa; Vasilakos, Athanasios V

    2013-12-01

    Ambient Intelligence (AmI) is a new paradigm in information technology aimed at empowering people's capabilities by the means of digital environments that are sensitive, adaptive, and responsive to human needs, habits, gestures, and emotions. This futuristic vision of daily environment will enable innovative human-machine interactions characterized by pervasive, unobtrusive and anticipatory communications. Such innovative interaction paradigms make ambient intelligence technology a suitable candidate for developing various real life solutions, including in the health care domain. This survey will discuss the emergence of ambient intelligence (AmI) techniques in the health care domain, in order to provide the research community with the necessary background. We will examine the infrastructure and technology required for achieving the vision of ambient intelligence, such as smart environments and wearable medical devices. We will summarize of the state of the art artificial intelligence methodologies used for developing AmI system in the health care domain, including various learning techniques (for learning from user interaction), reasoning techniques (for reasoning about users' goals and intensions) and planning techniques (for planning activities and interactions). We will also discuss how AmI technology might support people affected by various physical or mental disabilities or chronic disease. Finally, we will point to some of the successful case studies in the area and we will look at the current and future challenges to draw upon the possible future research paths.

  6. Using FML and fuzzy technology in adaptive ambient intelligent environments

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.

    2005-01-01

    Ambient Intelligence (AmI, shortly) gathers best re-sults from three key technologies, Ubiquitous Computing, Ubiq-uitous Communication, and Intelligent User Friendly Inter-faces. The functional and spatial distribution of tasks is a natu-ral thrust to employ multi-agent paradigm to design and

  7. El gasto público ambiental en Costa Rica

    Directory of Open Access Journals (Sweden)

    Gerardo Barrantes

    2011-09-01

    Full Text Available   En la actualidad, la sociedad se ha visto amenazada por los altos niveles de degradación ambiental experimentados en los últimos tiempos, y por el creciente agotamiento de los recursos naturales que han limitado la seguridad alimentaria en algunas regiones del país -y en algunos países del mundo- (FMI, 1996. La contaminación ambiental ha sobrepasado niveles críticos y se está revirtiendo en contra del mismo ser humano en términos de salud, disponibilidad de recursos y del disfrute que podía experimentar de la naturaleza (Brown, 1991.   El replanteamiento del estilo de desarrollo requiere la implementación de acciones alternativas que conduzcan a un mejoramiento de la calidad ambiental, a una recuperación de la base de recursos naturales y, en general, a niveles de calidad de la sociedad manifiesto en mejoras de la salud, educación, seguridad, etc. Para tal efecto, se pueden desarrollar un conjunto de indicadores que permitan evaluar el comportamiento y la evolución de los recursos naturales y la calidad ambiental; por ejemplo, indicadores para monitorear la calidad del aire y del agua.

  8. Amazonía y el medio ambiente

    OpenAIRE

    Mosquera Mesa, Ricardo

    2010-01-01

    Editorial: Amazonía: responsabilidad de todos -- Ecología y economía -- El patrimonio cultural amazónico -- La universidad y el liderazgo investigativo -- cooperación internacional -- Realizaciones y perspectivas -- Por una política de investigación ambiental

  9. effect of ambient levels of ozone on photosynthetic components

    African Journals Online (AJOL)

    ACSS

    To clarify the long-term effects of ambient levels of tropospheric ozone (O3) on ... (Rubisco), thus contributing to the reduction in net photosynthetic rate at the .... USA). During the measurements, atmospheric. CO2 concentrations, air ...... productivity and implications for climate change. Annual Review of Plant Biology 63:.

  10. Applying Formal Verification Techniques to Ambient Assisted Living Systems

    Science.gov (United States)

    Benghazi, Kawtar; Visitación Hurtado, María; Rodríguez, María Luisa; Noguera, Manuel

    This paper presents a verification approach based on timed traces semantics and MEDISTAM-RT [1] to check the fulfillment of non-functional requirements, such as timeliness and safety, and assure the correct functioning of the Ambient Assisted Living (AAL) systems. We validate this approach by its application to an Emergency Assistance System for monitoring people suffering from cardiac alteration with syncope.

  11. Amelie: A recombinant computing framework for ambient awareness

    NARCIS (Netherlands)

    Metaxas, G.; Markopoulos, P.; Aarts, E.H.L.; Tschlegi, M.

    2009-01-01

    This paper presents Amelie, a service oriented framework that supports the implementation of awareness systems. Amelie adopts the tenets of Recombinant computing to address an important non-functional requirement for Ambient Intelligence software, namely the heterogeneous combination of services and

  12. MODERN APPROACES IN THE CONTEXT OF AMBIENT INTELLIGENCE

    Directory of Open Access Journals (Sweden)

    Boldeanu Dana Maria

    2009-05-01

    Full Text Available Ambient Intelligence (AmI, as a new vision and concept of the tomorrow, gathers a few features regarding both the integration of technology in the environment and the capacity technology has to recognize the user and its context, the system capacity to i

  13. Smart Tales: An Awareness Game for Ambient Assisted Living

    NARCIS (Netherlands)

    Sernani, Paolo; Dalpiaz, Fabiano; Dragoni, Aldo Franco; Brinkkemper, Sjaak

    2015-01-01

    Despite the progress in ambient assisted living (AAL), the general audience is still mostly unaware of this term as well as of its purpose, enabling technologies, and potential. As a consequence, there are often misconceptions about AAL and smart homes, and the acceptance of AAL technologies is

  14. Modal Identification from Ambient Responses Using Frequency Domain Decomposition

    DEFF Research Database (Denmark)

    Brincker, Rune; Zhang, Lingmi; Andersen, Palle

    2000-01-01

    In this paper a new frequency domain technique is introduced for the modal identification from ambient responses, i.e. in the case where the modal parameters must be estimated without knowing the input exciting the system. By its user friendliness the technique is closely related to the classical...

  15. Three Mile Island ambient-air-temperature sensor measurements

    International Nuclear Information System (INIS)

    Fryer, M.O.

    1983-01-01

    Data from the ambient-air-temperature sensors in Three Mile Island-Unit 2 (TMI-2) reactor containment building are analyzed. The data were for the period of the hydrogen burn that was part of the TMI-2 accident. From the temperature data, limits are placed on the duration of the hydrogen burn

  16. Multimedia techniques for device and ambient intelligence: A continuing endeavor

    NARCIS (Netherlands)

    van den Broek, Egon

    2011-01-01

    The edited volume "Multimedia techniques for device and ambient intelligence" consists of two parts: i) an introduction to a variety of basic low level image processing techniques, leaving aside other modalities, and ii) work on high level, knowledge based processing, including interesting chapters

  17. Songs as Ambient Language Input in Phonology Acquisition

    Science.gov (United States)

    Au, Terry Kit-fong

    2013-01-01

    Children cannot learn to speak a language simply from occasional noninteractive exposure to native speakers' input (e.g., by hearing television dialogues), but can they learn something about its phonology? To answer this question, the present study varied ambient hearing experience for 126 5- to 7-year-old native Cantonese-Chinese speakers…

  18. An Ambient Awareness Tool for Supporting Supervised Collaborative Problem Solving

    Science.gov (United States)

    Alavi, H. S.; Dillenbourg, P.

    2012-01-01

    We describe an ambient awareness tool, named "Lantern", designed for supporting the learning process in recitation sections, (i.e., when students work in small teams on the exercise sets with the help of tutors). Each team is provided with an interactive lamp that displays their work status: the exercise they are working on, if they have…

  19. Damping Estimation Using Free Decays and Ambient Vibration Tests

    DEFF Research Database (Denmark)

    Magalhães, Filipe; Brincker, Rune; Cunha, Álvaro

    2007-01-01

    The accurate identification of modal damping ratios of Civil Engineering structures is a subject of major importance, as the amplitude of structural vibrations in resonance is inversely proportional to these coefficients. Their experimental identification can be performed either from ambient vibr...

  20. Human Aspects in Ambient Intelligence : Contemporary Challenges and Solutions

    NARCIS (Netherlands)

    Bosse, T.; Cook, D.J.; Neerincx, M.A.; Sadri, F.

    2013-01-01

    This book presents recent developments is the field of human aspects in Ambient Intelligence. This field, and the associated workshop series, addresses multidisciplinary aspects of AmI with human-directed disciplines such as psychology, social science, neuroscience and biomedical sciences. The aim

  1. Security and dependability for Ambient Intelligence: Informative but busy

    NARCIS (Netherlands)

    Law, Y.W.; Havinga, Paul J.M.

    2011-01-01

    The edited volume “Security and Dependability for Ambient Intelligence‿ is a comprehensive compilation of the research outcomes of the 3 year-long 7.8 million Euro European Framework Programme 6 project SERENITY (FP6-IST-2006-27587). At a time when Stuxnet and large scale data breaches at

  2. On Modal Parameter Estimates from Ambient Vibration Tests

    DEFF Research Database (Denmark)

    Agneni, A.; Brincker, Rune; Coppotelli, B.

    2004-01-01

    Modal parameter estimates from ambient vibration testing are turning into the preferred technique when one is interested in systems under actual loadings and operational conditions. Moreover, with this approach, expensive devices to excite the structure are not needed, since it can be adequately...

  3. An Ambient Agent To Support Depression Relapse Prevention

    NARCIS (Netherlands)

    Aziz, A.A.; Klein, M.C.A.; Treur, J.; Boldi, P.; Vizzari, G.

    2009-01-01

    One of the challenges for the patients with a history of unipolar depression is to stay healthy throughout their lifetime. In principle, with more prior onset cases, it escalates the risk of the patients to fall into a relapse. In this paper, an ambient agent based model to support patients from

  4. Effect of ambient levels of ozone on photosynthetic components and ...

    African Journals Online (AJOL)

    Effect of ambient levels of ozone on photosynthetic components and radical scavenging system in leaves of African cowpea varieties. ... The O3-induced significant reduction in catalase activity was observed in Blackeye at vegetative and reproductive growth stages; and in Asontem at reproductive growth stage. On the other ...

  5. Realidad de los sistemas de gestión ambiental

    Directory of Open Access Journals (Sweden)

    Sandra Constanza Escobar Cárdenas

    2009-07-01

    Full Text Available Este artículo presenta un análisis sobre la posibilidad de integrar los diferentes sistemas de gestión y la importancia que tiene los sistemas de gestión ambiental para el mejoramiento de las organizaciones. Luego de analizar comparativamente los modelos existentes, la autora presenta algunas recomendaciones de implementación.

  6. Activity recognition based on inertial sensors for ambient assisted living

    NARCIS (Netherlands)

    Davis, K.; Owusu, E.; Bastani, V.; Marcenaro, L.; Hu, J.; Regazzoni, C.; Feijs, L.M.G.

    2016-01-01

    Ambient Assisted Living (AAL) aims to create innovative technical solutions and services to support independent living among older adults, improve their quality of life and reduce the costs associated with health and social care. AAL systems provide health monitoring through sensor based

  7. Ambient effects on the electrical conductivity of carbon nanotubes

    DEFF Research Database (Denmark)

    Roch, Aljoscha; Greifzu, Moritz; Roch Talens, Esther

    2015-01-01

    We show that the electrical conductivity of single walled carbon nanotubes (SWCNT) networks is affected by oxygen and air humidity under ambient conditions by more than a magnitude. Later, we intentionally modified the electrical conductivity by functionalization with iodine and investigated...

  8. Ambient Sound-Based Collaborative Localization of Indeterministic Devices

    NARCIS (Netherlands)

    Kamminga, Jacob Wilhelm; Le Viet Duc, L Duc; Havinga, Paul J.M.

    2016-01-01

    Localization is essential in wireless sensor networks. To our knowledge, no prior work has utilized low-cost devices for collaborative localization based on only ambient sound, without the support of local infrastructure. The reason may be the fact that most low-cost devices are indeterministic and

  9. Storage beyond Three Hours at Ambient Temperature Alters the ...

    African Journals Online (AJOL)

    The effect of storage on stability of human breast milk was investigated in 30 lactating mothers. Samples stored for 3, 6 and 24 hours at ambient temperature of 302K (29°) were analysed for protein, lactose, pH, and microbial content. There were significant (p < 0.01) decreases in protein, lactose and pH upon storage for 6 ...

  10. Ambient Displays and Game Design Patterns for Social Learning

    NARCIS (Netherlands)

    Kelle, Sebastian; Börner, Dirk; Kalz, Marco; Specht, Marcus; Glahn, Christian

    2011-01-01

    Kelle, S., Börner, D., Kalz, M., Specht, M., & Glahn, C. (2010). Ambient Displays and Game Design Patterns for Social Learning. In B. Chang, T. Hirashima, & H. Ogata (Eds.), Joint Proceedings of the Work-in-Progress Poster and Invited Young Researcher Symposium for the 18th International Conference

  11. Characterization of selenium in ambient aerosols and primary emission sources.

    Science.gov (United States)

    De Santiago, Arlette; Longo, Amelia F; Ingall, Ellery D; Diaz, Julia M; King, Laura E; Lai, Barry; Weber, Rodney J; Russell, Armistead G; Oakes, Michelle

    2014-08-19

    Atmospheric selenium (Se) in aerosols was investigated using X-ray absorption near-edge structure (XANES) spectroscopy and X-ray fluorescence (XRF) microscopy. These techniques were used to determine the oxidation state and elemental associations of Se in common primary emission sources and ambient aerosols collected from the greater Atlanta area. In the majority of ambient aerosol and primary emission source samples, the spectroscopic patterns as well as the absence of elemental correlations suggest Se is in an elemental, organic, or oxide form. XRF microscopy revealed numerous Se-rich particles, or hotspots, accounting on average for ∼16% of the total Se in ambient aerosols. Hotspots contained primarily Se(0)/Se(-II). However, larger, bulk spectroscopic characterizations revealed Se(IV) as the dominant oxidation state in ambient aerosol, followed by Se(0)/Se(-II) and Se(VI). Se(IV) was the only observed oxidation state in gasoline, diesel, and coal fly ash, while biomass burning contained a combination of Se(0)/Se(-II) and Se(IV). Although the majority of Se in aerosols was in the most toxic form, the Se concentration is well below the California Environmental Protection Agency chronic exposure limit (∼20000 ng/m(3)).

  12. ambient volatile organic compounds pollution and ozone formation

    African Journals Online (AJOL)

    OLUMAYEDE

    2013-08-01

    Aug 1, 2013 ... Volatile organic compound (VOC) species react at different rate and exhibit differences in reactivity with respect to ozone formation in polluted urban atmosphere. To assess this, the variations pattern, reactivity relative to OH radical and ozone creation potential of ambient VOCs were investigated in field.

  13. Potential Ambient Energy-Harvesting Sources and Techniques

    Science.gov (United States)

    Yildiz, Faruk

    2009-01-01

    Ambient energy harvesting is also known as energy scavenging or power harvesting, and it is the process where energy is obtained from the environment. A variety of techniques are available for energy scavenging, including solar and wind powers, ocean waves, piezoelectricity, thermoelectricity, and physical motions. For example, some systems…

  14. Incorporating human aspects in ambient intelligence and smart environments

    NARCIS (Netherlands)

    Bosse, T.; Hoogendoorn, M.; Klein, M.C.A.; Lambalgen, R. van; Maanen, P.P. van; Treur, J.

    2011-01-01

    In this chapter, we propose to outline the scientific area that addresses Ambient Intelligence applications in which not only sensor data, but also knowledge from the human-directed sciences such as biomedical science, neuroscience, and psychological and social sciences is incorporated. This

  15. Autonomous composition of fuzzy granules in ambient intelligence scenarios

    NARCIS (Netherlands)

    Acampora, G.; Loia, V.; Vasilakos, A.V.; Pedrycz, W.; Bargiela, A.

    2009-01-01

    Pervasive and human-centric computing is beginning to be fact: with cell phones, laptops and handhelds, human beings can work pretty much anywhere. Ambient Intelligence (AmI) is a novel human-centric computer discipline based on three emergent technologies: Ubiquitous Computing, Ubiquitous

  16. Mapas de ruido: mecanismo de control del ruido ambiental

    Directory of Open Access Journals (Sweden)

    Kelvin Chávez García

    2012-12-01

    Full Text Available Los primeros mecanismos de control del ruido ambiental fueron los mapas acústicos. La Directiva Europea 2002149/CE del Parlamento Europeo y del Consejo de Evatuacián y Gestión del Ruido Ambiental, exige, a los estados miembros,  se midan los niveles de ruido, en su entorno local, con  el fin de contar con indicadores de ruido comunes. elaborar mapas de ruido y planes de acción destinados a reducir los efectos nocivos de ruido ambiental.  Las directrices para la elaboración de los  mapas  acústicos son en base a la normativa  Española sobre ruido. Los requerimientos  en base a la normativa, garantizan los resultados, lo cual permitirá elaborar los planes de acción. El estudio busca comunicar la necesidad de utilizar herramientas como  los  mapas  acústicos y ejercer un control de los  impactos por ruido ambiental.

  17. Indoor Localization for Optimized Ambient Assisted Living Services

    DEFF Research Database (Denmark)

    Mitev, Miroslav; Mihovska, Albena Dimitrova; Poulkov, Vladimir

    Indoor localization is very critical for the provision of Ambient Assisted Living (AAL) services, such as e-Health, smart home, etc. The success of deploying a real-time localization system depends on selecting the right performance characteristics. Bluetooth Low Energy (BLE) is a technology, which...

  18. Ambient RF energy scavenging: GSM and WLAN power density measurements

    NARCIS (Netherlands)

    Visser, H.J.; Reniers, A.C.F.; Theeuwes, J.A.C.

    2009-01-01

    To assess the feasibility of ambient RF energy scavenging, a survey of expected power density levels distant from GSM-900 and GSM-1800 base stations has been conducted and power density measurements have been performed in a WLAN environment. It appears that for distances ranging from 25 m to 100 m

  19. A seeded ambient temperature ferrite process for treatment of AMD ...

    African Journals Online (AJOL)

    A seeded ambient temperature ferrite process for treatment of AMD waters: magnetite formation in the presence and absence of calcium ions under steady state operation. ... promising for AMD treatment. Keywords: Ferrite process, Magnetite seed, Calcium interference, Acid mine drainage (WaterSA: 2003 29(2): 117-124) ...

  20. The Acceptance of Domestic Ambient Intelligence Appliances by Prospective Users

    NARCIS (Netherlands)

    Ben Allouch, Soumaya; van Dijk, Johannes A.G.M.; Peters, O.; Tokuda, Hideyuki; Beigl, Michael; Friday, Adrian; Bernheim Brush, A.J.; Tobe, Yoshito

    2009-01-01

    Ambient intelligence (AmI) is a growing interdisciplinary area where the focus is shifted towards users instead of merely emphasizing the technological opportunities of AmI. Different methods are employed to understand the adoption of AmI appliances by users. However, these are often small-scale