WorldWideScience

Sample records for alternatives analysis

  1. Business System Planning Project, Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    EVOSEVICH, S.

    2000-10-30

    The CHG Chief Information Officer (CIO) requested a study of alternatives to the current business system computing environment. This Business Systems Planning (BSP) Project Alternatives Analysis document presents an analysis of the current Project Controls, Work Management, and Business Management systems environment and alternative solutions that support the business functions. The project team has collected requirements and priorities from stakeholders in each business area and documented them in the BSP System Requirements Specification (SRS), RPP-6297. The alternatives analysis process identifies and measures possible solutions in each of the business process areas against the requirements as documented in the SRS. The team gathered input from both internal and external sources to identify and grade the possible solutions. This document captures the results of that activity and recommends a suite of software products. This study was to select the best product based on how well the product met the requirements, not to determine the platform or hardware environment that would be used. Additional analysis documentation can be found in BSP project files.

  2. Business System Planning Project, Alternatives Analysis

    International Nuclear Information System (INIS)

    The CHG Chief Information Officer (CIO) requested a study of alternatives to the current business system computing environment. This Business Systems Planning (BSP) Project Alternatives Analysis document presents an analysis of the current Project Controls, Work Management, and Business Management systems environment and alternative solutions that support the business functions. The project team has collected requirements and priorities from stakeholders in each business area and documented them in the BSP System Requirements Specification (SRS), RPP-6297. The alternatives analysis process identifies and measures possible solutions in each of the business process areas against the requirements as documented in the SRS. The team gathered input from both internal and external sources to identify and grade the possible solutions. This document captures the results of that activity and recommends a suite of software products. This study was to select the best product based on how well the product met the requirements, not to determine the platform or hardware environment that would be used. Additional analysis documentation can be found in BSP project files

  3. Economic analysis of alternative LLW disposal methods

    International Nuclear Information System (INIS)

    The Environmental Protection Agency (EPA) has evaluated the costs and benefits of alternative disposal technologies as part of its program to develop generally applicable environmental standards for the land disposal of low-level radioactive waste (LLW). Costs, population health effects and Critical Population Group (CPG) exposures resulting from alternative waste treatment and disposal methods were evaluated both in absolute terms and also relative to a base case (current practice). Incremental costs of the standard included costs for packaging, processing, transportation, and burial of waste. Benefits are defined in terms of reductions in the general population health risk (expected fatal cancers and genetic effects) evaluated over 10,000 years. A cost-effectiveness ratio, defined as the incremental cost per avoided health effect, was calculated for each alternative standard. The cost-effectiveness analysis took into account a number of waste streams, hydrogeologic and climatic region settings, and waste treatment and disposal methods. This paper describes the alternatives considered and preliminary results of the cost-effectiveness analysis. 15 references, 7 figures, 3 tables

  4. PAMS photo image retrieval prototype alternatives analysis

    Energy Technology Data Exchange (ETDEWEB)

    Conner, M.L.

    1996-04-30

    Photography and Audiovisual Services uses a system called the Photography and Audiovisual Management System (PAMS) to perform order entry and billing services. The PAMS system utilizes Revelation Technologies database management software, AREV. Work is currently in progress to link the PAMS AREV system to a Microsoft SQL Server database engine to provide photograph indexing and query capabilities. The link between AREV and SQLServer will use a technique called ``bonding.`` This photograph imaging subsystem will interface to the PAMS system and handle the image capture and retrieval portions of the project. The intent of this alternatives analysis is to examine the software and hardware alternatives available to meet the requirements for this project, and identify a cost-effective solution.

  5. Alternatives Analysis for the Resumption of Transient Testing Program

    Energy Technology Data Exchange (ETDEWEB)

    Lee Nelson

    2013-11-01

    An alternatives analysis was performed for resumption of transient testing. The analysis considered eleven alternatives – including both US international facilities. A screening process was used to identify two viable alternatives from the original eleven. In addition, the alternatives analysis includes a no action alternative as required by the National Environmental Policy Act (NEPA). The alternatives considered in this analysis included: 1. Restart the Transient Reactor Test Facility (TREAT) 2. Modify the Annular Core Research Reactor (ACRR) which includes construction of a new hot cell and installation of a new hodoscope. 3. No Action

  6. Hydrogen as alternative clean fuel: Economic analysis

    International Nuclear Information System (INIS)

    In analogy to biofuel production from biomasses, the electrolytic conversion of other renewable energies into hydrogen as an alternative clean fuel is considered. This solution allows the intermittent renewable energy sources, as photovoltaics and wind energy, to enhance their development and enlarge the role into conventional fuel market. A rough economic analysis of hydrogen production line shows the costs, added by electrolysis and storage stages, can be recovered by properly accounting for social and environmental costs due to whole cycle of conventional fuels, from production to use. So, in a perspective of attaining the economic competitiveness of renewable energy, the hydrogen, arising from intermittent renewable energy sources, will be able to compete in the energy market with conventional fuels, making sure that their substitution will occur in a significant amount and the corresponding environment

  7. Sodium Bearing Waste Processing Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, James Anthony; Palmer, Brent J; Perry, Keith Joseph

    2003-12-01

    A multidisciplinary team gathered to develop a BBWI recommendation to DOE-ID on the processing alternatives for the sodium bearing waste in the INTEC Tank Farm. Numerous alternatives were analyzed using a rigorous, systematic approach. The data gathered were evaluated through internal and external peer reviews for consistency and validity. Three alternatives were identified to be top performers: Risk-based Calcination, MACT to WIPP Calcination and Cesium Ion Exchange. A dual-path through early Conceptual design is recommended for MACT to WIPP Calcination and Cesium Ion Exchange since Risk-based Calcination does not require design. If calcination alternatives are not considered based on giving Type of Processing criteria significantly greater weight, the CsIX/TRUEX alternative follows CsIX in ranking. However, since CsIX/TRUEX shares common uncertainties with CsIX, reasonable backups, which follow in ranking, are the TRUEX and UNEX alternatives. Key uncertainties must be evaluated by the decision-makers to choose one final alternative. Those key uncertainties and a path forward for the technology roadmapping of these alternatives is provided.

  8. Sodium Bearing Waste Processing Alternatives Analysis

    International Nuclear Information System (INIS)

    A multidisciplinary team gathered to develop a BBWI recommendation to DOE-ID on the processing alternatives for the sodium bearing waste in the INTEC Tank Farm. Numerous alternatives were analyzed using a rigorous, systematic approach. The data gathered were evaluated through internal and external peer reviews for consistency and validity. Three alternatives were identified to be top performers: Risk-based Calcination, MACT to WIPP Calcination and Cesium Ion Exchange. A dual-path through early Conceptual design is recommended for MACT to WIPP Calcination and Cesium Ion Exchange since Risk-based Calcination does not require design. If calcination alternatives are not considered based on giving Type of Processing criteria significantly greater weight, the CsIX/TRUEX alternative follows CsIX in ranking. However, since CsIX/TRUEX shares common uncertainties with CsIX, reasonable backups, which follow in ranking, are the TRUEX and UNEX alternatives. Key uncertainties must be evaluated by the decision-makers to choose one final alternative. Those key uncertainties and a path forward for the technology roadmapping of these alternatives is provided

  9. Experiment and Analysis of Car Alternator for Wind Turbine Application

    Directory of Open Access Journals (Sweden)

    Pudji Irasari

    2012-03-01

    Full Text Available This paper discusses experiment and analysis to find out the feasibility of a car alternator to be used as a generator for wind turbine. The experiment was conducted twice. The first experiment was to characterize the alternator to determine the mechanical transmission ratio. In this experiment the alternator was driven by a lathe machine and its output power was supplied to charge a battery. In the second experiment the alternator was integrated with the turbine blades and they were tested as a unit system. In both experiments, the electric generation of alternator was executed with fixed excitation current method. The correlation between the alternator characteristic and the tip speed ratio gives the mechanical transmission ratio of 1 : 3. The experiment results show that the efficiency of alternator is around 50% and cut-in wind speed (after correction is 6.35 m/s indicating that alternator is not feasible for wind turbine system application. 

  10. Supplementary vote; analysis, applications, and alternatives

    NARCIS (Netherlands)

    Kolk, van der Henk

    2008-01-01

    The Supplementary Vote (SV) was invented as a compromise between the two-round runoff system and the alternative vote. This paper shows that in many ways it is not. Under SV voters rank order a limited number of candidates. If one candidate is supported by over 50% of the voters he/she is declared t

  11. Spent Nuclear Fuel Alternative Technology Decision Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Shedrow, C.B.

    1999-11-29

    The Westinghouse Savannah River Company (WSRC) made a FY98 commitment to the Department of Energy (DOE) to recommend a technology for the disposal of aluminum-based spent nuclear fuel (SNF) at the Savannah River Site (SRS). The two technologies being considered, direct co-disposal and melt and dilute, had been previously selected from a group of eleven potential SNF management technologies by the Research Reactor Spent Nuclear Fuel Task Team chartered by the DOE''s Office of Spent Fuel Management. To meet this commitment, WSRC organized the SNF Alternative Technology Program to further develop the direct co-disposal and melt and dilute technologies and ultimately provide a WSRC recommendation to DOE on a preferred SNF alternative management technology.

  12. Alternative crypto currency analysis and mining

    OpenAIRE

    Ballek, Miroslav

    2014-01-01

    Over the past few years, bitcoin has become a globally successful and much followed project. It is a kind of decentralized digital currency, which functions based on mathematical-cryptographical principles and is created using a computationally intensive process called mining. Thousands of other alternative crypto currencies exist, which have so far stayed unnoticed by the media, even though they outperform bitcoin in many aspects. The central topic of this paper is the extensive ecosystem of...

  13. Competitive Cost Analysis of Alternative Powertrain Technologies.

    OpenAIRE

    Redelbach, Martin; Propfe, Bernd; Friedrich, Horst E.

    2012-01-01

    This paper examines the cost competitiveness of different electrified propulsion technologies from hybrid cars to full battery electric vehicles in the time horizon 2010 to 2020. The assessment shows that the current TCO gaps for alternative drivetrains will increasingly converge over time mainly driven by decreasing production cost. However, the cost-efficiency of different powertrain architectures depends highly on the mileage a user expects to drive per year. In the mid-run, hybrid elec...

  14. Alternative Phrases Theoretical Analysis and Practical Application

    OpenAIRE

    Bierner, Gann

    2001-01-01

    "All right, but apart from the sanitation, the medicine, education, wine, public order, irrigation, roads, the fresh-water system and public health, what have the Romans ever done for us?" (Monty Python, The Life of Brian) Alternative phrases identify selected elements from a set and subject them to particular scrutiny with respect to the sentence's predicate. For instance, in the above example, sanitation, medicine, etc. are all identified as elements in the set of thin...

  15. Summary: analysis of alternative FBR development strategies

    International Nuclear Information System (INIS)

    This report summarizes the comparative evaluation of alternative strategies for the development of the commercial fast breeder reactor (FBR) in the United States. For planning purposes, a range of possible FBR development paths called strategies were selected for evaluation. These strategies, designed to be technically and economically feasible, were expressed in terms of the timing and nature of facilities/research and development programs required to reach full power operation of the first commercial FBR. Four of the seven strategies resulted in a large (1457 MWe) FBR as an end point, the other three in a 1000-MWe plant. Probability distributions were calculated for total strategy costs and time to completion. For the seven strategies analyzed, the costs (discounted 1980 dollars) ranged from $1.8 billion to $4.9 billion; the completion times ranged from 24 to 55 years

  16. Propensity Score Analysis: An Alternative Statistical Approach for HRD Researchers

    Science.gov (United States)

    Keiffer, Greggory L.; Lane, Forrest C.

    2016-01-01

    Purpose: This paper aims to introduce matching in propensity score analysis (PSA) as an alternative statistical approach for researchers looking to make causal inferences using intact groups. Design/methodology/approach: An illustrative example demonstrated the varying results of analysis of variance, analysis of covariance and PSA on a heuristic…

  17. Advanced Post-Irradiation Examination Capabilities Alternatives Analysis Report

    Energy Technology Data Exchange (ETDEWEB)

    Jeff Bryan; Bill Landman; Porter Hill

    2012-12-01

    An alternatives analysis was performed for the Advanced Post-Irradiation Capabilities (APIEC) project in accordance with the U.S. Department of Energy (DOE) Order DOE O 413.3B, “Program and Project Management for the Acquisition of Capital Assets”. The Alternatives Analysis considered six major alternatives: ? No Action ? Modify Existing DOE Facilities – capabilities distributed among multiple locations ? Modify Existing DOE Facilities – capabilities consolidated at a few locations ? Construct New Facility ? Commercial Partnership ? International Partnerships Based on the alternatives analysis documented herein, it is recommended to DOE that the advanced post-irradiation examination capabilities be provided by a new facility constructed at the Materials and Fuels Complex at the Idaho National Laboratory.

  18. Nonparametric Production Analysis under Alternative Price Conditions

    OpenAIRE

    Laurens Cherchye; Timo Kuosmanen; Thierry Post

    2001-01-01

    The literature on non-parametric production analysis has formulated tests for profit maximizing behavior that do not require a parametric specification of technology. Negative test results have conventionally been interpreted as inefficiency, or have been attributed to data perturbations. In this paper, we exploit the possibility that negative test results reveal violations of the underlying neoclassical assumption that prices are exogenously fixed and perfectly certain. We propose non-parame...

  19. ALTERNATIVES ANALYSIS FOR SELECTING ET #3 SITE

    Energy Technology Data Exchange (ETDEWEB)

    Collard, L.; Hamm, L.

    2012-02-13

    Engineered trenches (ETs) are considered to be a cost-effective method for disposing Low Level Waste (LLW). Based on waste forecasts from waste generators, the last engineered trench in operation (ET No.2) is anticipated to close in FY14, requiring development of a new ET. Solid Waste requested that SRNL develop an assessment report that reviews four disposal options for this new ET (ET No.3) and determine which option would provide the 'best' Performance Assessment (PA) disposal limits for LLW (Appendix A). Those four options (see option footprint locations in Figure 1-1) are: (1) Disposal at grade on TRU Pads 7-13 where soil would be mounded over waste packages; (2) Excavation at a slightly modified SLIT No.13 location - near the Used Equipment Storage Area; (3) Excavation at a modified SLIT No.12 location - near the 643-26E Naval Reactor Component Disposal Area; and (4) Excavation east of TRU Pad No.26 that replaces northeast portions of four slit trench (ST) disposal units in the eastern set of STs. The assessment consisted of both quantitative and qualitative analyses. The quantitative analysis captured key aspects that were readily quantifiable and had predictable impacts on limits and doses. A simplified modeling strategy stemming from current Special Analysis (SA) practices was employed. Both inventory capacity for a specific nuclide (a quasi-inventory limit) and overall performance for specified inventory mixtures (doses resulting from historical inventories) were considered. The qualitative analysis evaluated other key aspects based on engineering judgment in the form of pros and cons.

  20. Analysis of the Alternate Extrusion and Multiaxial Compression Process

    OpenAIRE

    Kwapisz M.; Knapiński M.; Dyja H.; Kawałek A.

    2015-01-01

    The paper present the results of numerical simulations of the alternate indirect extrusion and multiaxial compression process, performed using commercial software designed for the thermomechanical analysis of plastic working processes, Forge 2009. The novel method of alternate indirect extrusion and multiaxial compression, proposed by the authors, is characterized by the occurrence of strain states in the material being plastically worked, which are similar to those occurring in the equal cha...

  1. Analysis of the Alternate Extrusion and Multiaxial Compression Process

    Directory of Open Access Journals (Sweden)

    Kwapisz M.

    2015-04-01

    Full Text Available The paper present the results of numerical simulations of the alternate indirect extrusion and multiaxial compression process, performed using commercial software designed for the thermomechanical analysis of plastic working processes, Forge 2009. The novel method of alternate indirect extrusion and multiaxial compression, proposed by the authors, is characterized by the occurrence of strain states in the material being plastically worked, which are similar to those occurring in the equal channel angular pressing and cyclic extrusion compression processes.

  2. Alternate approaches to vibration and shock analysis using NASTRAN

    Science.gov (United States)

    Denver, R. E.; Menichello, J. M.

    1977-01-01

    A method that derives an approximate equivalent static load to a base excitation shock analysis is described. The transient analysis in the current level of NASTRAN, level 16, does not directly provide for either input acceleration forcing functions or enforced boundary displacement. In the suggested alternate analysis format, equivalent force input functions are applied to the constrained locations by using the artifice of placing a large mass, with respect to the total system mass, at the desired acceleration input points. This shortcut static analysis approach is presented to approximate the expensive and time consuming dynamics analysis approach to the base excitation shock analysis.

  3. Cost-Benefit Analysis for Alternatives to Aliphatic Isocyanate Polyurethanes

    Science.gov (United States)

    Lewis, Pattie

    2007-01-01

    NASA and Air Force Space Command (AFSPC) have similar missions and therefore similar facilities and structures in similar environments. The standard practice for protecting metallic substrates in atmospheric environments is the application of an applied coating system. The most common topcoats used in coating systems are polyurethanes that contain isocyanates. Isocyanates are classified as potential human carcinogens and are known to cause cancer in animals. The primary objective of this effort was to demonstrate and validate alternatives to aliphatic isocyanate polyurethanes resulting in one or more isocyanate-free coatings qualified for use at AFSPC and NASA installations participating in this project. This Cost-Benefit Analysis (CBA) quantifies the estimated capital and process costs of coating alternatives and cost savings relative to the current coatings. The estimates in this CBA are to be used for assessing the relative merits of the selected alternatives. The actual economic effects at any specific facility will depend on the alternative material or technology implemented, the number of actual applications converted, future workloads, and other factors . The participants initially considered eighteen (18) alternative coatings as described in the Potential Alternatives Report entitled Potential Alternatives Report for Validation of Alternatives to Aliphatic Isocyanate Polyurethanes, prepared by ITB. Of those, 8 alternatives were selected for testing in accordance with the Joint Test Protocol entitled Joint Test Protocol for Validation of Alternatives to Aliphatic Isocyanate Polyurethanes, and the Field Test Plan entitled Field Evaluations Test Plan for Validation of Alternatives 10 Aliphatic Isocyanate Polyurethanes, both of which were prepared by ITB. A joint Test Report entitled Joint Test Report for Validation of Alternatives to Aliphatic Isocyanate Polyurethanes, prepared by ITB, documents the results of the laboratory and field testing, as well as any

  4. A Review of Nonparametric Alternatives to Analysis of Covariance.

    Science.gov (United States)

    Olejnik, Stephen F.; Algina, James

    1985-01-01

    Five distribution-free alternatives to parametric analysis of covariance are presented and demonstrated: Quade's distribution-free test, Puri and Sen's solution, McSweeney and Porter's rank transformation, Burnett and Barr's rank difference scores, and Shirley's general linear model solution. The results of simulation studies regarding Type I…

  5. Decisions, Science, and Values: Crafting Regulatory Alternatives Analysis.

    Science.gov (United States)

    Malloy, Timothy; Blake, Ann; Linkov, Igor; Sinsheimer, Peter

    2015-12-01

    Emerging "prevention-based" approaches to chemical regulation seek to minimize the use of toxic chemicals by mandating or directly incentivizing the adoption of viable safer alternative chemicals or processes. California and Maine are beginning to implement such programs, requiring manufacturers of consumer products containing certain chemicals of concern to identify and evaluate potential safer alternatives. In the European Union, the REACH program imposes similar obligations on manufacturers of certain substances of very high concern. Effective prevention-based regulation requires regulatory alternatives analysis (RAA), a methodology for comparing and evaluating the regulated chemical or process and its alternatives across a range of relevant criteria. RAA has both public and private dimensions. To a significant degree, alternatives analysis is an aspect of product design; that is, the process by which private industry designs the goods it sells. Accordingly, an RAA method should reflect the attributes of well-crafted product design tools used by businesses. But RAA adds health and environmental objectives to the mix of concerns taken into account by the product designer. Moreover, as part of a prevention-based regulatory regime, it implicates important public values such as legitimacy, equity, public engagement, and accountability. Thus, an RAA should reflect both private standards and public values, and be evaluated against them. This article adopts that perspective, identifying an integrated set of design principles for RAA, and illustrating the application of those principles. PMID:26299695

  6. Comparative analysis of traditional and alternative energy sources

    International Nuclear Information System (INIS)

    The presented thesis with designation of Comparing analysis of traditional and alternative energy resources includes, on basis of theoretical information source, research in firm, internal data, trends in company development and market, description of the problem and its application. Theoretical information source is dedicated to the traditional and alternative energy resources, reserves of it, trends in using and development, the balance of it in the world, EU and in Slovakia as well. Analysis of the thesis is reflecting profile of the company and the thermal pump market evaluation using General Electric method. While the company is implementing, except other products, the thermal pumps on geothermal energy base and surround energy base (air), the mission of the comparing analysis is to compare traditional energy resources with thermal pump from the ecological, utility and economic side of it. The results of the comparing analysis are resumed in to the SWOT analysis. The part of the thesis includes t he questionnaire offer for effectiveness improvement and customer satisfaction analysis, and expected possibilities of alternative energy resources assistance (benefits) from the government and EU funds. (authors)

  7. 100-KE REACTOR CORE REMOVAL PROJECT ALTERNATIVE ANALYSIS WORKSHOP REPORT

    Energy Technology Data Exchange (ETDEWEB)

    HARRINGTON RA

    2010-01-15

    On December 15-16, 2009, a 100-KE Reactor Core Removal Project Alternative Analysis Workshop was conducted at the Washington State University Consolidated Information Center, Room 214. Colburn Kennedy, Project Director, CH2M HILL Plateau Remediation Company (CHPRC) requested the workshop and Richard Harrington provided facilitation. The purpose of the session was to select the preferred Bio Shield Alternative, for integration with the Thermal Shield and Core Removal and develop the path forward to proceed with project delivery. Prior to this workshop, the S.A. Robotics (SAR) Obstruction Removal Alternatives Analysis (565-DLV-062) report was issued, for use prior to and throughout the session, to all the team members. The multidisciplinary team consisted ofrepresentatives from 100-KE Project Management, Engineering, Radcon, Nuclear Safety, Fire Protection, Crane/Rigging, SAR Project Engineering, the Department of Energy Richland Field Office, Environmental Protection Agency, Washington State Department of Ecology, Defense Nuclear Facility Safety Board, and Deactivation and Decommission subject matter experts from corporate CH2M HILL and Lucas. Appendix D contains the workshop agenda, guidelines and expectations, opening remarks, and attendance roster going into followed throughout the workshop. The team was successful in selecting the preferred alternative and developing an eight-point path forward action plan to proceed with conceptual design. Conventional Demolition was selected as the preferred alternative over two other alternatives: Diamond Wire with Options, and Harmonic Delamination with Conventional Demolition. The teams preferred alternative aligned with the SAR Obstruction Removal Alternative Analysis report conclusion. However, the team identified several Path Forward actions, in Appendix A, which upon completion will solidify and potentially enhance the Conventional Demolition alternative with multiple options and approaches to achieve project delivery

  8. Complementary and alternative exercise for fibromyalgia: a meta-analysis

    OpenAIRE

    Mist SD; Firestone KA; Jones KD

    2013-01-01

    Scott David Mist, Kari Firestone, Kim Dupree Jones Fibromyalgia Research and Treatment Group, School of Nursing, Oregon Health and Science University, Portland, OR, USA Abstract: Complementary and alternative medicine includes a number of exercise modalities, such as tai chi, qigong, yoga, and a variety of lesser-known movement therapies. A meta-analysis of the current literature was conducted estimating the effect size of the different modalities, study quality and bias, and adverse events....

  9. Systematic analysis of alternative first exons in plant genomes

    Directory of Open Access Journals (Sweden)

    Zeng Changqing

    2007-10-01

    Full Text Available Abstract Background Alternative splicing (AS contributes significantly to protein diversity, by selectively using different combinations of exons of the same gene under certain circumstances. One particular type of AS is the use of alternative first exons (AFEs, which can have consequences far beyond the fine-tuning of protein functions. For example, AFEs may change the N-termini of proteins and thereby direct them to different cellular compartments. When alternative first exons are distant, they are usually associated with alternative promoters, thereby conferring an extra level of gene expression regulation. However, only few studies have examined the patterns of AFEs, and these analyses were mainly focused on mammalian genomes. Recent studies have shown that AFEs exist in the rice genome, and are regulated in a tissue-specific manner. Our current understanding of AFEs in plants is still limited, including important issues such as their regulation, contribution to protein diversity, and evolutionary conservation. Results We systematically identified 1,378 and 645 AFE-containing clusters in rice and Arabidopsis, respectively. From our data sets, we identified two types of AFEs according to their genomic organisation. In genes with type I AFEs, the first exons are mutually exclusive, while most of the downstream exons are shared among alternative transcripts. Conversely, in genes with type II AFEs, the first exon of one gene structure is an internal exon of an alternative gene structure. The functionality analysis indicated about half and ~19% of the AFEs in Arabidopsis and rice could alter N-terminal protein sequences, and ~5% of the functional alteration in type II AFEs involved protein domain addition/deletion in both genomes. Expression analysis indicated that 20~66% of rice AFE clusters were tissue- and/or development- specifically transcribed, which is consistent with previous observations; however, a much smaller percentage of Arabidopsis

  10. Genome-wide analysis of alternative splicing in Chlamydomonas reinhardtii

    Directory of Open Access Journals (Sweden)

    Thomas Julie

    2010-02-01

    Full Text Available Abstract Background Genome-wide computational analysis of alternative splicing (AS in several flowering plants has revealed that pre-mRNAs from about 30% of genes undergo AS. Chlamydomonas, a simple unicellular green alga, is part of the lineage that includes land plants. However, it diverged from land plants about one billion years ago. Hence, it serves as a good model system to study alternative splicing in early photosynthetic eukaryotes, to obtain insights into the evolution of this process in plants, and to compare splicing in simple unicellular photosynthetic and non-photosynthetic eukaryotes. We performed a global analysis of alternative splicing in Chlamydomonas reinhardtii using its recently completed genome sequence and all available ESTs and cDNAs. Results Our analysis of AS using BLAT and a modified version of the Sircah tool revealed AS of 498 transcriptional units with 611 events, representing about 3% of the total number of genes. As in land plants, intron retention is the most prevalent form of AS. Retained introns and skipped exons tend to be shorter than their counterparts in constitutively spliced genes. The splice site signals in all types of AS events are weaker than those in constitutively spliced genes. Furthermore, in alternatively spliced genes, the prevalent splice form has a stronger splice site signal than the non-prevalent form. Analysis of constitutively spliced introns revealed an over-abundance of motifs with simple repetitive elements in comparison to introns involved in intron retention. In almost all cases, AS results in a truncated ORF, leading to a coding sequence that is around 50% shorter than the prevalent splice form. Using RT-PCR we verified AS of two genes and show that they produce more isoforms than indicated by EST data. All cDNA/EST alignments and splice graphs are provided in a website at http://combi.cs.colostate.edu/as/chlamy. Conclusions The extent of AS in Chlamydomonas that we observed is much

  11. Elk Valley Rancheria Energy Efficiency and Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Ed Wait, Elk Valley Rancheria; Frank Ziano & Associates, Inc.

    2011-11-30

    Elk Valley Rancheria; Tribe; renewable energy; energy options analysis. The Elk Valley Rancheria, California ('Tribe') is a federally recognized Indian tribe located in Del Norte County, California, in the northwestern corner of California. The Tribe, its members and Tribal enterprises are challenged by increasing energy costs and undeveloped local energy resources. The Tribe currently lacks an energy program. The Tribal government lacked sufficient information to make informed decisions about potential renewable energy resources, energy alternatives and other energy management issues. To meet this challenge efficiently, the Tribe contracted with Frank Zaino and Associates, Inc. to help become more energy self-sufficient, by reducing their energy costs and promoting energy alternatives that stimulate economic development. Frank Zaino & Associates, Inc. provided a high level economic screening analysis based on anticipated electric and natural gas rates. This was in an effort to determine which alternative energy system will performed at a higher level so the Tribe could reduce their energy model by 30% from alternative fuel sources. The feasibility study will identify suitable energy alternatives and conservation methods that will benefit the Tribe and tribal community through important reductions in cost. The lessons learned from these conservation efforts will yield knowledge that will serve a wider goal of executing energy efficiency measures and practices in Tribal residences and business facilities. Pacific Power is the provider of electrical power to the four properties under review at $ 0.08 per Kilowatt-hour (KWH). This is a very low energy cost compared to alternative energy sources. The Tribe used baseline audits to assess current and historic energy usage at four Rancheria owned facilities. Past electric and gas billing statements were retained for review for the four buildings that will be audited. A comparative assessment of the various

  12. Low-Level Waste Disposal Alternatives Analysis Report

    Energy Technology Data Exchange (ETDEWEB)

    Timothy Carlson; Kay Adler-Flitton; Roy Grant; Joan Connolly; Peggy Hinman; Charles Marcinkiewicz

    2006-09-01

    This report identifies and compares on-site and off-site disposal options for the disposal of contract-handled and remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Potential disposal options are screened for viability by waste type resulting in a short list of options for further consideration. The most crediable option are selected after systematic consideration of cost, schedule constraints, and risk. In order to holistically address the approach for low-level waste disposal, options are compiled into comprehensive disposal schemes, that is, alternative scenarios. Each alternative scenario addresses the disposal path for all low-level waste types over the period of interest. The alternative scenarios are compared and ranked using cost, risk and complexity to arrive at the recommended approach. Schedule alignment with disposal needs is addressed to ensure that all waste types are managed appropriately. The recommended alternative scenario for the disposal of low-level waste based on this analysis is to build a disposal facility at the Idaho National Laboratory Site.

  13. Low-Level Waste Disposal Alternatives Analysis Report

    International Nuclear Information System (INIS)

    This report identifies and compares on-site and off-site disposal options for the disposal of contract-handled and remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Potential disposal options are screened for viability by waste type resulting in a short list of options for further consideration. The most credible option are selected after systematic consideration of cost, schedule constraints, and risk. In order to holistically address the approach for low-level waste disposal, options are compiled into comprehensive disposal schemes, that is, alternative scenarios. Each alternative scenario addresses the disposal path for all low-level waste types over the period of interest. The alternative scenarios are compared and ranked using cost, risk and complexity to arrive at the recommended approach. Schedule alignment with disposal needs is addressed to ensure that all waste types are managed appropriately. The recommended alternative scenario for the disposal of low-level waste based on this analysis is to build a disposal facility at the Idaho National Laboratory Site

  14. Latent common manifold learning with alternating diffusion: analysis and applications

    CERN Document Server

    Talmon, Ronen

    2016-01-01

    The analysis of data sets arising from multiple sensors has drawn significant research attention over the years. Traditional methods, including kernel-based methods, are typically incapable of capturing nonlinear geometric structures. We introduce a latent common manifold model underlying multiple sensor observations for the purpose of multimodal data fusion. A method based on alternating diffusion is presented and analyzed; we provide theoretical analysis of the method under the latent common manifold model. To exemplify the power of the proposed framework, experimental results in several applications are reported.

  15. Tradeoff Analysis for Combat Service Support Wireless Communications Alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Burnette, John R.; Thibodeau, Christopher C.; Greitzer, Frank L.

    2002-02-28

    As the Army moves toward more mobile and agile forces and continued sustainment of numerous high-cost legacy logistics management systems, the requirement for wireless connectivity and a wireless network to supporting organizations has become ever more critical. There are currently several Army communications initiatives underway to resolve this wireless connectivity issue. However, to fully appreciate and understand the value of these initiatives, a Tradeoff Analysis is needed. The present study seeks to identify and assess solutions. The analysis identified issues that impede Interim Brigade Combat Team (IBCT) communication system integration and outlined core requirements for sharing of logistics data between the field and Army battle command systems. Then, the analysis examined wireless communication alternatives as possible solutions for IBCT logistics communications problems. The current baseline system was compared with possible alternatives involving tactical radio systems, wireless/near term digital radio, cellular satellite, and third-generation (3G) wireless technologies. Cellular satellite and 3G wireless technologies offer clear advantages and should be considered for later IBCTs.

  16. Analysis of alternatives for immobilized low activity waste disposal

    Energy Technology Data Exchange (ETDEWEB)

    Burbank, D.A.

    1997-10-28

    This report presents a study of alternative disposal system architectures and implementation strategies to provide onsite near-surface disposal capacity to receive the immobilized low-activity waste produced by the private vendors. The analysis shows that a flexible unit strategy that provides a suite of design solutions tailored to the characteristics of the immobilized low-activity waste will provide a disposal system that best meets the program goals of reducing the environmental, health, and safety impacts; meeting the schedule milestones; and minimizing the life-cycle cost of the program.

  17. Stability analysis of abandoned drift for alternative utilization

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Bo-Hyun; Yoo, In-Kol; Kim, Moon-Seob; Kim, Ryang-Hee [Korea Resources Corp., Seoul(Korea); Yang, Hyung-Sik [Chonnam National University, Kwangju(Korea); Lee, Hi-Keun [Seoul National University, Seoul(Korea); Kim, Jae-Dong [Kangwon National University, Chuncheon(Korea)

    2000-04-30

    This study was performed to find the way that is alternative utilization of the drift that had been abandoned by coal industry promotion. To determine whether abandoned drift would be maintained without supports or not, the safety inspection that consists of drift survey, laboratory tests, rock mass classification and numerical analysis was performed. According to the result, there were discontinuity sets those had unfavorable orientation to the drift and rock mass around the drift had generally poor condition. So, it would be much difficult that abandoned drift is maintained without supports. (author). 15 refs., 8 tabs., 7 figs.

  18. Conflict analysis and management alternative for the Manayunk Canal

    International Nuclear Information System (INIS)

    The solution of a water resource allocation problem by an alternative social arrangement is presented. Classical Austrian economic theory and the new resources economics provide both theoretical and practical evidence to support the development of well-defined, private property rights to the water resource in question. A conflict analysis demonstrates that management of the Manayunk Canal by a firm would reconcile existing confrontations through compromise use of the water resource. Benefit and cost calculations show that a compromise among industrial and recreational interests, currently competing in the political arena, would increase social benefits

  19. Analysis of alternatives for immobilized low-activity waste disposal

    International Nuclear Information System (INIS)

    This report presents a study of alternative disposal system architectures and implementation strategies to provide onsite near-surface disposal capacity to receive the immobilized low-activity waste produced by the private vendors. The analysis shows that a flexible unit strategy that provides a suite of design solutions tailored to the characteristics of the immobilized low-activity waste will provide a disposal system that best meets the program goals of reducing the environmental, health, and safety impacts; meeting the schedule milestones; and minimizing the life-cycle cost of the program

  20. Analysis of Alternatives for Risk Assessment Methodologies and Tools

    Energy Technology Data Exchange (ETDEWEB)

    Nachtigal, Noel M. [Sandia National Lab. (SNL-CA), Livermore, CA (United States). System Analytics; Fruetel, Julia A. [Sandia National Lab. (SNL-CA), Livermore, CA (United States). Systems Research and Analysis; Gleason, Nathaniel J. [Sandia National Lab. (SNL-CA), Livermore, CA (United States). Systems Research and Analysis; Helms, Jovana [Sandia National Lab. (SNL-CA), Livermore, CA (United States). Systems Research and Analysis; Imbro, Dennis Raymond [Sandia National Lab. (SNL-CA), Livermore, CA (United States). Systems Research and Analysis; Sumner, Matthew C. [Sandia National Lab. (SNL-CA), Livermore, CA (United States). Systems Research and Analysis

    2013-10-01

    The purpose of this document is to provide a basic overview and understanding of risk assessment methodologies and tools from the literature and to assess the suitability of these methodologies and tools for cyber risk assessment. Sandia National Laboratories (SNL) performed this review in support of risk modeling activities performed for the Stakeholder Engagement and Cyber Infrastructure Resilience (SECIR) division of the Department of Homeland Security (DHS) Office of Cybersecurity and Communications (CS&C). The set of methodologies and tools covered in this document is not intended to be exhaustive; instead, it focuses on those that are commonly used in the risk assessment community. The classification of methodologies and tools was performed by a group of analysts with experience in risk analysis and cybersecurity, and the resulting analysis of alternatives has been tailored to address the needs of a cyber risk assessment.

  1. Life-cycle analysis of alternative aviation fuels in GREET

    Energy Technology Data Exchange (ETDEWEB)

    Elgowainy, A.; Han, J.; Wang, M.; Carter, N.; Stratton, R.; Hileman, J.; Malwitz, A.; Balasubramanian, S. (Energy Systems)

    2012-07-23

    The Greenhouse gases, Regulated Emissions, and Energy use in Transportation (GREET) model, developed at Argonne National Laboratory, has been expanded to include well-to-wake (WTWa) analysis of aviation fuels and aircraft. This report documents the key WTWa stages and assumptions for fuels that represent alternatives to petroleum jet fuel. The aviation module in GREET consists of three spreadsheets that present detailed characterizations of well-to-pump and pump-to-wake parameters and WTWa results. By using the expanded GREET version (GREET1{_}2011), we estimate WTWa results for energy use (total, fossil, and petroleum energy) and greenhouse gas (GHG) emissions (carbon dioxide, methane, and nitrous oxide) for (1) each unit of energy (lower heating value) consumed by the aircraft or (2) each unit of distance traveled/ payload carried by the aircraft. The fuel pathways considered in this analysis include petroleum-based jet fuel from conventional and unconventional sources (i.e., oil sands); Fisher-Tropsch (FT) jet fuel from natural gas, coal, and biomass; bio-jet fuel from fast pyrolysis of cellulosic biomass; and bio-jet fuel from vegetable and algal oils, which falls under the American Society for Testing and Materials category of hydroprocessed esters and fatty acids. For aircraft operation, we considered six passenger aircraft classes and four freight aircraft classes in this analysis. Our analysis revealed that, depending on the feedstock source, the fuel conversion technology, and the allocation or displacement credit methodology applied to co-products, alternative bio-jet fuel pathways have the potential to reduce life-cycle GHG emissions by 55-85 percent compared with conventional (petroleum-based) jet fuel. Although producing FT jet fuel from fossil feedstock sources - such as natural gas and coal - could greatly reduce dependence on crude oil, production from such sources (especially coal) produces greater WTWa GHG emissions compared with petroleum jet

  2. Life-Cycle Analysis of Alternative Aviation Fuels in GREET

    Energy Technology Data Exchange (ETDEWEB)

    Elgowainy, A. [Argonne National Lab. (ANL), Argonne, IL (United States); Han, J. [Argonne National Lab. (ANL), Argonne, IL (United States); Wang, M. [Argonne National Lab. (ANL), Argonne, IL (United States); Carter, N. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Stratton, R. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Hileman, J. [Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States); Malwitz, A. [Volpe National Transportation Systems Center, Cambridge, MA (United States); Balasubramanian, S. [Volpe National Transportation Systems Center, Cambridge, MA (United States)

    2012-06-01

    The Greenhouse gases, Regulated Emissions, and Energy use in Transportation (GREET) model, developed at Argonne National Laboratory, has been expanded to include well-to-wake (WTWa) analysis of aviation fuels and aircraft. This report documents the key WTWa stages and assumptions for fuels that represent alternatives to petroleum jet fuel. The aviation module in GREET consists of three spreadsheets that present detailed characterizations of well-to-pump and pump-to-wake parameters and WTWa results. By using the expanded GREET version (GREET1_2011), we estimate WTWa results for energy use (total, fossil, and petroleum energy) and greenhouse gas (GHG) emissions (carbon dioxide, methane, and nitrous oxide) for (1) each unit of energy (lower heating value) consumed by the aircraft or(2) each unit of distance traveled/ payload carried by the aircraft. The fuel pathways considered in this analysis include petroleum-based jet fuel from conventional and unconventional sources (i.e., oil sands); Fisher-Tropsch (FT) jet fuel from natural gas, coal, and biomass; bio-jet fuel from fast pyrolysis of cellulosic biomass; and bio-jet fuel from vegetable and algal oils, which falls under the American Society for Testing and Materials category of hydroprocessed esters and fatty acids. For aircraft operation, we considered six passenger aircraft classes and four freight aircraft classes in this analysis. Our analysis revealed that, depending on the feedstock source, the fuel conversion technology, and the allocation or displacement credit methodology applied to co-products, alternative bio-jet fuel pathways have the potential to reduce life-cycle GHG emissions by 55–85 percent compared with conventional (petroleum-based) jet fuel. Although producing FT jet fuel from fossil feedstock sources — such as natural gas and coal — could greatly reduce dependence on crude oil, production from such sources (especially coal) produces greater WTWa GHG emissions compared with petroleum jet

  3. Nutrient analysis of the Beef Alternative Merchandising cuts.

    Science.gov (United States)

    Desimone, T L; Acheson, R A; Woerner, D R; Engle, T E; Douglass, L W; Belk, K E

    2013-03-01

    The objective of this study was to generate raw and cooked nutrient composition data to identify Quality Grade differences in proximate values for eight Beef Alternative Merchandising (BAM) cuts. The data generated will be used to update the nutrient data in the USDA National Nutrient Database for Standard Reference (SR). Beef Rib, Oven-Prepared, Beef Loin, Strip Loin, and Beef Loin, Top Sirloin Butt subprimals were collected from a total of 24 carcasses from four packing plants. The carcasses were a combination of USDA Yield Grades 2 (n=12) and 3 (n=12), USDA Quality Grades upper two-thirds Choice (n=8), low Choice (n=8), and Select (n=8), and two genders, steer (n=16) and heifer (n=8). After aging, subprimals were fabricated into the BAM cuts, dissected, and nutrient analysis was performed. Sample homogenates from each animal were homogenized and composited for analysis of the following: proximate analysis, long chain and trans-fatty acids, conjugated linoleic acid, total cholesterol, vitamin B-12, and selenium. This study identified seven BAM cuts from all three Quality Grades that qualify for USDA Lean; seven Select cuts that qualify for USDA Extra Lean; and three Select cuts that qualify for the American Heart Association's Heart Healthy Check. PMID:23261533

  4. Analysis of alternative strategies for energy conservation in new buildings

    Energy Technology Data Exchange (ETDEWEB)

    Fang, J.M.; Tawil, J.J.

    1980-12-01

    Building Energy Performance Standards (BEPS) were mandated by the Energy Conservation Standards for New Buildings Act of 1976 (Title III of Energy Conservation and Production Act) to promote energy efficiency and the use of renewable resources in new buildings. The report analyzes alternative Federal strategies and their component policy instruments and recommends a strategy for achieving the goals of the Act. The concern is limited to space conditioning (heating, cooling, and lighting) and water heating. The policy instruments considered include greater reliance on market forces; research and development; information, education and demonstration programs; tax incentives and sanctions; mortgage and finance programs; and regulations and standards. The analysis starts with an explanation of the barriers to energy conservation in the residential and commercial sectors. Individual policy instruments are then described and evaluated with respect to energy conservation, economic efficiency, equity, political impacts, and implementation and other transitional impacts. Five possible strategies are identified: (1) increased reliance on the market place; (2) energy consumption tax and supply subsidies; (3) BEPS with no sanctions and no incentives; (4) BEPS with sanctions and incentives (price control); and (5) BEPS with sanctions and incentives (no price controls). A comparative analysis is performed. Elements are proposed for inclusion in a comprehensive strategy for conservation in new buildings. (MCW)

  5. Progressive collapse analysis using updated models for alternate path analysis after a blast

    Science.gov (United States)

    Eskew, Edward; Jang, Shinae; Bertolaccini, Kelly

    2016-04-01

    Progressive collapse is of rising importance within the structural engineering community due to several recent cases. The alternate path method is a design technique to determine the ability of a structure to sustain the loss of a critical element, or elements, and still resist progressive collapse. However, the alternate path method only considers the removal of the critical elements. In the event of a blast, significant damage may occur to nearby members not included in the alternate path design scenarios. To achieve an accurate assessment of the current condition of the structure after a blast or other extreme event, it may be necessary to reduce the strength or remove additional elements beyond the critical members designated in the alternate path design method. In this paper, a rapid model updating technique utilizing vibration measurements is used to update the structural model to represent the real-time condition of the structure after a blast occurs. Based upon the updated model, damaged elements will either have their strength reduced, or will be removed from the simulation. The alternate path analysis will then be performed, but only utilizing the updated structural model instead of numerous scenarios. After the analysis, the simulated response from the analysis will be compared to failure conditions to determine the buildings post-event condition. This method has the ability to incorporate damage to noncritical members into the analysis. This paper will utilize numerical simulations based upon a unified facilities criteria (UFC) example structure subjected to an equivalent blast to validate the methodology.

  6. An Analysis of State Policies Connected with Alternative School Programs

    Science.gov (United States)

    Bielefeld, William C.; Stubblefield, Anna; Templeton, Zach

    2009-01-01

    Many school districts offer alternative education programs for their students who do not succeed in the regular classroom. At the high school level, many of these programs are separated from the regular schools. It seemed that many districts were establishing alternative education programs, but there were differences in the types of programs that…

  7. Ipsative measurement and the analysis of organizational values: an alternative approach for data analysis

    NARCIS (Netherlands)

    F.M. van Eijnatten; L.A. van der Ark; S.S. Holloway

    2015-01-01

    In this paper, the analysis and test of ipsative data will be discussed, and some alternative methods will be suggested. Following a review of the literature about ipsative measurement, the Competing Values Framework will be presented as a major application in the field of organizational culture and

  8. Remote-Handled Low Level Waste Disposal Project Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    David Duncan

    2010-10-01

    This report identifies, evaluates, and compares alternatives for meeting the U.S. Department of Energy’s mission need for management of remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Each alternative identified in the Mission Need Statement for the Remote-Handled Low-Level Waste Treatment Project is described and evaluated for capability to fulfill the mission need. Alternatives that could meet the mission need are further evaluated and compared using criteria of cost, risk, complexity, stakeholder values, and regulatory compliance. The alternative for disposal of remote-handled low-level waste that has the highest confidence of meeting the mission need and represents best value to the government is to build a new disposal facility at the Idaho National Laboratory Site.

  9. Long-term-consequence analysis of no action alternative 2

    International Nuclear Information System (INIS)

    This report is a supplement to the Waste Isolation Pilot Plant (WIPP) Disposal-Phase Supplemental Environmental Impact Statement. Data and information is described which pertains to estimated impacts from postulated long-term release of radionuclides and hazardous constituents from alpha-bearing wastes stored at major generator/storage sites after loss of institutional control (no action alternative 2). Under this alternative, wastes would remain at the generator sites and not be emplaced at WIPP

  10. Long-term-consequence analysis of no action alternative 2

    Energy Technology Data Exchange (ETDEWEB)

    Buck, J.W.; Bagaasen, L.M.; Staven, L.H.; Serne, R.J. [and others

    1996-07-01

    This report is a supplement to the Waste Isolation Pilot Plant (WIPP) Disposal-Phase Supplemental Environmental Impact Statement. Data and information is described which pertains to estimated impacts from postulated long-term release of radionuclides and hazardous constituents from alpha-bearing wastes stored at major generator/storage sites after loss of institutional control (no action alternative 2). Under this alternative, wastes would remain at the generator sites and not be emplaced at WIPP.

  11. Alternatives generation and analysis for phase I intermediate waste feed staging system design requirements

    Energy Technology Data Exchange (ETDEWEB)

    Britton, M.D.

    1996-10-02

    This document provides; a decision analysis summary; problem statement; constraints, requirements, and assumptions; decision criteria; intermediate waste feed staging system options and alternatives generation and screening; intermediate waste feed staging system design concepts; intermediate waste feed staging system alternative evaluation and analysis; and open issues and actions.

  12. Cost-Benefit Analysis For Alternative Low-Emission Surface Preparation/ Depainting Technologies for Structural Steel

    Science.gov (United States)

    Lewis, Pattie

    2007-01-01

    Stennis Space Center (SSC), Kennedy Space Center (KSC) and Air Force Space Command (AFSPC) identified particulate emissions and waste generated from the depainting process of steel structures as hazardous materials to be eliminated or reduced. A Potential Alternatives Report, Potential Alternatives Report for Validation of Alternative Low Emission Surface Preparation/Depainting Technologies for Structural Steel, provided a technical analyses of identified alternatives to the current coating removal processes, criteria used to select alternatives for further analysis, and a list of those alternatives recommended for testing. The initial coating removal alternatives list was compiled using literature searches and stakeholder recommendations. The involved project participants initially considered approximately 13 alternatives. In late 2003, core project members selected the following depainting processes to be further evaluated: (1) Plastic Blast Media-Quickstrip(R)-A. (2) Hard Abrasive-Steel-Magic(R). (3) Sponge Blasting-Sponge-Jet(R). (4) Liquid Nitrogen-NItroJet(R). (5) Mechanical Removal with Vacuum Attachment-DESCO and OCM Clean-Air (6) Laser Coating Removal Alternatives were tested in accordance with the Joint Test Protocol for Validation of Alternative Low-Emission Surface Preparation/Depainting Technologies for Structural Steel, and the Field Evaluation Test Plan for Validation of Alternative Low-Emission Surface Preparation/Depainting Technologies for Structural Steel. Results of the testing are documented in the Joint Test Report. This Cost-Benefit Analysis (CBA) focuses on the three alternatives (Quickstrip(R)-A, SteelMagic (R), and Sponge-Jet(R)) that were considered viable alternatives for large area operations based on the results of the field demonstration and lab testing. This CBA was created to help participants determine if implementation of the candidate alternatives is economically justified. Each of the alternatives examined reduced Environmental

  13. Alternatives to Multilevel Modeling for the Analysis of Clustered Data

    Science.gov (United States)

    Huang, Francis L.

    2016-01-01

    Multilevel modeling has grown in use over the years as a way to deal with the nonindependent nature of observations found in clustered data. However, other alternatives to multilevel modeling are available that can account for observations nested within clusters, including the use of Taylor series linearization for variance estimation, the design…

  14. Alternative Nonvolatile Residue Analysis with Contaminant Identification Project

    Science.gov (United States)

    Loftin, Kathleen (Compiler); Summerfield, Burton (Compiler); Thompson, Karen (Compiler); Mullenix, Pamela (Compiler); Zeitlin, Nancy (Compiler)

    2015-01-01

    Cleanliness verification is required in numerous industries including spaceflight ground support, electronics, medical and aerospace. Currently at KSC requirement for cleanliness verification use solvents that environmentally unfriendly. This goal of this project is to produce an alternative cleanliness verification technique that is both environmentally friendly and more cost effective.

  15. 40 CFR 68.28 - Alternative release scenario analysis.

    Science.gov (United States)

    2010-07-01

    .... 68.28 Section 68.28 Protection of Environment ENVIRONMENTAL PROTECTION AGENCY (CONTINUED) AIR... alternative release scenarios: (1) The five-year accident history provided in § 68.42; and (2) Failure... applicable as part of current practices. Proprietary models that account for the modeling conditions may...

  16. Energy demand analysis in the workshop on alternative energy strategies

    Energy Technology Data Exchange (ETDEWEB)

    Carhart, S C

    1978-04-01

    The Workshop on Alternative Energy Strategies, conducted from 1974 through 1977, was an international study group formed to develop consistent national energy alternatives within a common analytical framework and global assumptions. A major component of this activity was the demand program, which involved preparation of highly disaggregated demand estimates based upon estimates of energy-consuming activities and energy requirements per unit of activity reported on a consistent basis for North America, Europe, and Japan. Comparison of the results of these studies reveals that North America requires more energy per unit of activity in many consumption categories, that major improvements in efficiency will move North America close to current European and Japanese efficiencies, and that further improvements in European and Japanese efficiencies may be anticipated as well. When contrasted with expected availabilities of fuels, major shortfalls of oil relative to projected demands emerge in the eighties and nineties. Some approaches to investment in efficiency improvements which will offset these difficulties are discussed.

  17. Economic Environmental Valuation: An Analysis of Limitations and Alternatives.

    OpenAIRE

    Paul Knights, Jeroen Admiraal, Ada Wossink, Prasenjit Banerjee, John O’Neill and Michael Scott

    2013-01-01

    The structure of this report is as follows. In Section 2 we introduce economic methods for environmental and biodiversity valuation, and in particular the total economic value model. Sections 3 through 7 will advance five distinct but related classes of criticisms of economic environmental valuation, and in each we will suggest the way in which alternative valuation methods contain the resources to help address those criticisms.

  18. Peptide-Centric Proteome Analysis: An Alternative Strategy for the Analysis of Tandem Mass Spectrometry Data

    Energy Technology Data Exchange (ETDEWEB)

    Ting, Ying S.; Egertson, Jarrett D.; Payne, Samuel H.; Kim, Sangtae; MacLean, Brendan; Kall, Lukas; Aebersold, Ruedi; Smith, Richard D.; Noble, William; MacCoss, Michael

    2015-09-01

    In mass spectrometry-based bottom-up proteomics, data-independent acquisition (DIA) is an emerging technique due to its comprehensive and unbiased sampling of precursor ions. However, current DIA methods use wide precursor isolation windows, resulting in co- fragmentation and complex mixture spectra. Thus, conventional database searching tools that identify peptides by interpreting individual MS/MS spectra are inherently limited in analyzing DIA data. Here we discuss an alternative approach, peptide-centric analysis, which tests directly for the presence and absence of query peptides. We discuss how peptide-centric analysis resolves some limitations of traditional spectrum-centric analysis, and we outline the benefits of peptide-centric analysis in general.

  19. Verbal rating of alternative research reactors using fuzzy decision analysis

    International Nuclear Information System (INIS)

    An approach is introduced here for making decisions about alternative research reactor types based on their compatibility with the environment of Saudi Arabia and is applied to the choice between pool, light water tank, heavy water tank, and TRIGA reactors. The method is based on the fuzzy decision theory, and it allows for consideration of the availability of required local resources as well as ease of acquisition of imported resources, community acceptance, and future expandability. The use of fuzzy decision theory can overcome the numerical precision of a decision maker's judgment by allowing verbal rating and weighting for each attribute and subattribute

  20. Alternative pseudodifferential analysis with an application to modular forms

    CERN Document Server

    Unterberger, André

    2008-01-01

    This volume introduces an entirely new pseudodifferential analysis on the line, the opposition of which to the usual (Weyl-type) analysis can be said to reflect that, in representation theory, between the representations from the discrete and from the (full, non-unitary) series, or that between modular forms of the holomorphic and substitute for the usual Moyal-type brackets. This pseudodifferential analysis relies on the one-dimensional case of the recently introduced anaplectic representation and analysis, a competitor of the metaplectic representation and usual analysis. Besides researchers and graduate students interested in pseudodifferential analysis and in modular forms, the book may also appeal to analysts and physicists, for its concepts making possible the transformation of creation-annihilation operators into automorphisms, simultaneously changing the usual scalar product into an indefinite but still non-degenerate one.

  1. Summary of Plutonium-238 Production Alternatives Analysis Final Report

    Energy Technology Data Exchange (ETDEWEB)

    James Werner; Wade E. Bickford; David B. Lord; Chadwick D. Barklay

    2013-03-01

    The Team implemented a two-phase evaluation process. During the first phase, a wide variety of past and new candidate facilities and processing methods were assessed against the criteria established by DOE for this assessment. Any system or system element selected for consideration as an alternative within the project to reestablish domestic production of Pu-238 must meet the following minimum criteria: Any required source material must be readily available in the United States, without requiring the development of reprocessing technologies or investments in systems to separate material from identified sources. It must be cost, schedule, and risk competitive with existing baseline technology. Any identified facilities required to support the concept must be available to the program for the entire project life cycle (notionally 35 years, unless the concept is so novel as to require a shorter duration). It must present a solution that can generate at least 1.5 Kg of Pu-238 oxide per year, for at least 35 years. It must present a low-risk, near-term solution to the National Aeronautics and Space Administration’s urgent mission need. DOE has implemented this requirement by eliminating from project consideration any alternative with key technologies at less than Technology Readiness Level 5. The Team evaluated the options meeting these criteria using a more detailed assessment of the reasonable facility variations and compared them to the preferred option, which consists of target irradiation at the Advanced Test Reactor (ATR) and the High Flux Isotope Reactor (HFIR), target fabrication and chemical separations processing at the ORNL Radiochemical Engineering Development Center, and neptunium 237 storage at the Materials and Fuels Complex at INL. This preferred option is consistent with the Records of Decision from the earlier National Environmental Policy Act (NEPA) documentation

  2. Use of multi-criteria decision analysis in regulatory alternatives analysis: a case study of lead free solder.

    Science.gov (United States)

    Malloy, Timothy F; Sinsheimer, Peter J; Blake, Ann; Linkov, Igor

    2013-10-01

    Regulators are implementing new programs that require manufacturers of products containing certain chemicals of concern to identify, evaluate, and adopt viable, safer alternatives. Such programs raise the difficult question for policymakers and regulated businesses of which alternatives are "viable" and "safer." To address that question, these programs use "alternatives analysis," an emerging methodology that integrates issues of human health and environmental effects with technical feasibility and economic impact. Despite the central role that alternatives analysis plays in these programs, the methodology itself is neither well-developed nor tailored to application in regulatory settings. This study uses the case of Pb-based bar solder and its non-Pb-based alternatives to examine the application of 2 multi-criteria decision analysis (MCDA) methods to alternatives analysis: multi-attribute utility analysis and outranking. The article develops and evaluates an alternatives analysis methodology and supporting decision-analysis software for use in a regulatory context, using weighting of the relevant decision criteria generated from a stakeholder elicitation process. The analysis produced complete rankings of the alternatives, including identification of the relative contribution to the ranking of each of the highest level decision criteria such as human health impacts, technical feasibility, and economic feasibility. It also examined the effect of variation in data conventions, weighting, and decision frameworks on the outcome. The results indicate that MCDA can play a critical role in emerging prevention-based regulatory programs. Multi-criteria decision analysis methods offer a means for transparent, objective, and rigorous analysis of products and processes, providing regulators and stakeholders with a common baseline understanding of the relative performance of alternatives and the trade-offs they present.

  3. Analysis of generalized Schwarz alternating procedure for domain decomposition

    Energy Technology Data Exchange (ETDEWEB)

    Engquist, B.; Zhao, Hongkai [Univ. of California, Los Angeles, CA (United States)

    1996-12-31

    The Schwartz alternating method(SAM) is the theoretical basis for domain decomposition which itself is a powerful tool both for parallel computation and for computing in complicated domains. The convergence rate of the classical SAM is very sensitive to the overlapping size between each subdomain, which is not desirable for most applications. We propose a generalized SAM procedure which is an extension of the modified SAM proposed by P.-L. Lions. Instead of using only Dirichlet data at the artificial boundary between subdomains, we take a convex combination of u and {partial_derivative}u/{partial_derivative}n, i.e. {partial_derivative}u/{partial_derivative}n + {Lambda}u, where {Lambda} is some {open_quotes}positive{close_quotes} operator. Convergence of the modified SAM without overlapping in a quite general setting has been proven by P.-L.Lions using delicate energy estimates. The important questions remain for the generalized SAM. (1) What is the most essential mechanism for convergence without overlapping? (2) Given the partial differential equation, what is the best choice for the positive operator {Lambda}? (3) In the overlapping case, is the generalized SAM superior to the classical SAM? (4) What is the convergence rate and what does it depend on? (5) Numerically can we obtain an easy to implement operator {Lambda} such that the convergence is independent of the mesh size. To analyze the convergence of the generalized SAM we focus, for simplicity, on the Poisson equation for two typical geometry in two subdomain case.

  4. Waste management facility accident analysis (WASTE ACC) system: software for analysis of waste management alternatives

    International Nuclear Information System (INIS)

    This paper describes the Waste Management Facility Accident Analysis (WASTEunderscoreACC) software, which was developed at Argonne National Laboratory (ANL) to support the US Department of Energy's (DOE's) Waste Management (WM) Programmatic Environmental Impact Statement (PEIS). WASTEunderscoreACC is a decision support and database system that is compatible with Microsoft reg-sign Windows trademark. It assesses potential atmospheric releases from accidents at waste management facilities. The software provides the user with an easy-to-use tool to determine the risk-dominant accident sequences for the many possible combinations of process technologies, waste and facility types, and alternative cases described in the WM PEIS. In addition, its structure will allow additional alternative cases and assumptions to be tested as part of the future DOE programmatic decision-making process. The WASTEunderscoreACC system demonstrates one approach to performing a generic, systemwide evaluation of accident risks at waste management facilities. The advantages of WASTEunderscoreACC are threefold. First, the software gets waste volume and radiological profile data that were used to perform other WM PEIS-related analyses directly from the WASTEunderscoreMGMT system. Second, the system allows for a consistent analysis across all sites and waste streams, which enables decision makers to understand more fully the trade-offs among various policy options and scenarios. Third, the system is easy to operate; even complex scenario runs are completed within minutes

  5. Alternative Analysis of Classifications of Universal Managerial Functions

    Directory of Open Access Journals (Sweden)

    Chornii Heorhii M.

    2014-03-01

    Full Text Available The article is devoted to theoretical justification of classification of general managerial functions. Critical analysis of scientific works of eminent specialists in management gave a possibility to find out that classification of functions of the subject of management should be based on the objective cyclic change of the state of information by three stages: appearance of information, its concentration and transformation into a programme of specific actions. The article offers a classification model of the integrated managerial activity in social and mixed systems, which includes differentiation of managerial functions into three orders. Providing information, decision making and realisation of the decision are functions of the second order (basic in this model, while accounting, analysis, goal setting, planning, organisation, management and control – are, consequently, functions of the third order (derivatives. The article considers one specific example from management practice with the aim of verification of the author’s opinion regarding the processed classification of managerial functions.

  6. Alternative Approaches for Rating INDCs: a Comparative Analysis

    OpenAIRE

    Davide, Marinella; Vesco, Paola

    2016-01-01

    The “Intended nationally determined contributions” (INDCs) communicated by both developing and developed countries represent a crucial element of the Paris agreement. This paper aims at analysing the INDCs submitted by Parties, through the different tools and approaches proposed by the research community. In particular, our analysis looks at the different ways to assess the effectiveness of the proposed emission reduction pledges, both in terms of aggregate and national efforts. However, we a...

  7. Dynamic Analysis of Alternative Suspension Systems for Sport Motorcycles

    OpenAIRE

    Ramirez, Ciro Moreno

    2015-01-01

    In this thesis, VehicleSim multi-body software is used to extend and modify an existing motorcycle model by including different non-conventional suspension systems. Girder and Hossack double wishbones front suspension systems are designed, implemented and tested. Using a synthesis of mechanism methodology, they are designed with different kinematic configurations that allow different behaviours of the motorcycle front end. By means of CAD tools and finite element analysis, realistic three dim...

  8. Configurational analysis as an alternative way of modeling sales response

    OpenAIRE

    Aarnio, Susanna

    2013-01-01

    Objectives of the Study The objectives of the study are both managerial and methodological. On the one hand, the aim is to apply a novel research approach, fuzzy set qualitative comparative analysis or fsQCA (see f. ex. Ragin, 2000; Rihoux & Ragin, 2009), to sales response modeling and thus, create a response model for the case company to identify complex, configurational causalities affecting the company's sales volumes within the chosen product category. On the other hand, to goal is to ...

  9. Low-level waste management alternatives and analysis in DOE`s programmatic environmental impact statement

    Energy Technology Data Exchange (ETDEWEB)

    Gerstein, J.S. [Dept. of Energy, Washington, DC (United States). Office of Environmental Restoration and Waste Management

    1993-03-01

    The Department of Energy is preparing a Programmatic Environmental Impact Statement (PEIS) for the Environmental Restoration and Waste Management Program. The PEIS has been divided into an Environmental Restoration section and a Waste Management section. Each section has a unique set of alternatives. This paper will focus on the waste management alternatives and analysis. The set of alternatives for waste management has been divided into waste categories. These categories are: high-level waste, transuranic waste, low-level waste, low-level mixed waste, greater-than-class C and low-level waste from commercial sources, hazardous waste, and spent nuclear fuel. This paper will discuss the alternatives and analytical approach that will be used to evaluate these alternatives for the low-level waste section. Although the same alternatives will be considered for all waste types, the analysis will be performed separately for each waste type. In the sections that follow, information will be provided on waste management configurations, the analysis of waste management alternatives, waste types and locations, facility and transportation activities, the facility and transportation impacts assessment, and the compilation of impacts.

  10. Analysis of radwaste management alternatives during dismantling of Ignalina NPP systems with low level contamination

    Energy Technology Data Exchange (ETDEWEB)

    Poskas, Gintautas [Lithuanian Energy Institute, Kaunas (Lithuania). Nuclear Engineering Lab.; Kaunas Univ. of Technology (Lithuania); Poskas, Povilas; Simonis, Audrius [Lithuanian Energy Institute, Kaunas (Lithuania). Nuclear Engineering Lab.

    2013-12-15

    Ignalina NPP was operating two RBMK-1500 reactors which are under decommissioning now. In this paper, analysis on radwaste management alternatives during the dismantling of systems with low level contamination and different types of components in buildings 117/1 and V1 are presented. After situation analysis and collection of the primary information related to components' physical and radiological characteristics, location and other data, two alternatives for radwaste management during the dismantling were formulated and evaluated: the first one (A1) when the decontamination of the dismantled components is performed (if it is reasonable), and the second one (A2) when no decontamination of the dismantled components is performed and after the dismantling, the components are routed to appropriate waste storage or disposal sites. To select the preferable alternative, MCDA method - AHP (Analytic Hierarchy Process) is applied. Hierarchical lists of decision criteria, necessary for assessment of alternatives performance, are formulated. Quantitative decision criteria values for these alternatives are calculated using software DECRAD, which was developed by Lithuanian Energy Institute Nuclear Engineering Laboratory. Qualitative decision criteria are evaluated using expert judgment. Analysis results show that alternative A1 has a preference against alternative A2. (orig.)

  11. Functional and evolutionary analysis of alternatively spliced genes is consistent with an early eukaryotic origin of alternative splicing

    DEFF Research Database (Denmark)

    Irimia, Manuel; Rukov, Jakob Lewin; Penny, David;

    2007-01-01

    , and may therefore predate multicellularity, is still unknown. To better understand the origin and evolution of alternative splicing and its usage in diverse organisms, we studied alternative splicing in 12 eukaryotic species, comparing rates of alternative splicing across genes of different functional...... classes, cellular locations, intron/exon structures and evolutionary origins. RESULTS: For each species, we find that genes from most functional categories are alternatively spliced. Ancient genes (shared between animals, fungi and plants) show high levels of alternative splicing. Genes with products...... expressed in the nucleus or plasma membrane are generally more alternatively spliced while those expressed in extracellular location show less alternative splicing. We find a clear correspondence between incidence of alternative splicing and intron number per gene both within and between genomes. In general...

  12. Coarse-grid-CFD. An advantageous alternative to subchannel analysis

    International Nuclear Information System (INIS)

    In the 1960th to 80th when current GEN II reactor technology was developed, the only possible approach was to use one-dimensional subchannel analysis to compute the flow inside a fuel bundle so that the subchannel scale could be resolved. For simulations of the whole reactor core either system codes or homogenization were employed. In system codes resolution of individual assemblies was the state of the art. Homogenization used porous media equations simulations and averaged the thermohydraulics on reactor core scale. Current potent computing power allows using Computational Fluid Dynamics (CFD) to simulate individual fuel assemblies. Yet the large number of fuel assemblies within the core forbids exploiting CFD for core wide simulation. We propose to combine ideas of subchannel analysis and CFD to develop a new methodology which takes advantage of the fast development of commercial CFD software and the efficiency of subchannel analysis. In this methodology was first applied to simulate a wire-wrap fuel bundle of the High Performance Light Water Reactor (HPLWR). Computations using an inviscid Euler solver on an extremely coarse grid were tuned to predict the true thermohydraulics by adding volumetric forces. These forces represent the non-resolved sub-grid physics. The volumetric forces cannot be measured directly. However, they can be accessed from detailed CFD simulations resolving all relevant physics. Parameterization of these subgrid forces can be realized analogous to models in subchannel codes. In the present work we extend the methodology to the open source solver OpenFOAM and a specific hexagonal fuel assembly which is studied in the framework of liquid metal cooled GEN IV reactor concepts. (orig.)

  13. Preliminary analysis of alternative fuel cycles for proliferation evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Steindler, M. J.; Ripfel, H. C.F.; Rainey, R. H.

    1977-01-01

    The ERDA Division of Nuclear Research and Applications proposed 67 nuclear fuel cycles for assessment as to their nonproliferation potential. The object of the assessment was to determine which fuel cycles pose inherently low risk for nuclear weapon proliferation while retaining the major benefits of nuclear energy. This report is a preliminary analysis of these fuel cycles to develop the fuel-recycle data that will complement reactor data, environmental data, and political considerations, which must be included in the overall evaluation. This report presents the preliminary evaluations from ANL, HEDL, ORNL, and SRL and is the basis for a continuing in-depth study. (DLC)

  14. Economic analysis of alternative options in CANDU fuel cycle

    International Nuclear Information System (INIS)

    In this study, fuel cycle options for CANDU reactor were studied. Three main options in a CANDU fuel cycle involve use of : (1) natural uranium (0.711 weight percent U-235) fuel, (2) slightly enriched uranium (1.2 weight percent U-235) fuel, and (3) recovered uranium (0.83 weight percent U-235) fuel from light water reactor spent fuel. ORIGEN-2 computer code was used to identify composition of the spent fuel for each option , including the standard LWR fuel (3.3 weight percent U-235). Uranium and plutonium credit calculations were performed by using ORIGEN-2 output. WIMSD-5 computer code was used to determine maximum discharge burnup values for each case. Cost estimations were carried out using specially-developed computer programs. Comparison of levelized costs for the fuel cycle options and sensitivity analysis for the cost components are also presented

  15. Multi-Criteria Analysis of Alternative Energy Supply Solutions to Public Nearly Zero Energy Buildings

    Directory of Open Access Journals (Sweden)

    Giedrius Šiupšinskas

    2013-12-01

    Full Text Available The article analyzes energy supply alternatives for modernised public nearly zero energy buildings. The paper examines alternative energy production systems such as heat pumps (air-water and ground-water, solar collectors, adsorption cooling, biomass boiler, solar photovoltaic, wind turbines and combinations of these systems. The simulation of the analysed building energy demand for different energy production alternatives has been performed using TRNSYS modelling software. In order to determine an optimal energy supply variant, the estimated results of energy, environmental, and economic evaluation have been converted into non-dimensional variables (3E using multi-criteria analysis.Article in Lithuanian

  16. INEEL Subsurface Disposal Area CERCLA-based Decision Analysis for Technology Screening and Remedial Alternative Evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Parnell, G. S.; Kloeber, Jr. J.; Westphal, D; Fung, V.; Richardson, John Grant

    2000-03-01

    A CERCLA-based decision analysis methodology for alternative evaluation and technology screening has been developed for application at the Idaho National Engineering and Environmental Laboratory WAG 7 OU13/14 Subsurface Disposal Area (SDA). Quantitative value functions derived from CERCLA balancing criteria in cooperation with State and Federal regulators are presented. A weighted criteria hierarchy is also summarized that relates individual value function numerical values to an overall score for a specific technology alternative.

  17. Comprehensive analysis of alternative splicing in rice and comparative analyses with Arabidopsis

    Directory of Open Access Journals (Sweden)

    Mount Stephen M

    2006-12-01

    Full Text Available Abstract Background Recently, genomic sequencing efforts were finished for Oryza sativa (cultivated rice and Arabidopsis thaliana (Arabidopsis. Additionally, these two plant species have extensive cDNA and expressed sequence tag (EST libraries. We employed the Program to Assemble Spliced Alignments (PASA to identify and analyze alternatively spliced isoforms in both species. Results A comprehensive analysis of alternative splicing was performed in rice that started with >1.1 million publicly available spliced ESTs and over 30,000 full length cDNAs in conjunction with the newly enhanced PASA software. A parallel analysis was performed with Arabidopsis to compare and ascertain potential differences between monocots and dicots. Alternative splicing is a widespread phenomenon (observed in greater than 30% of the loci with transcript support and we have described nine alternative splicing variations. While alternative splicing has the potential to create many RNA isoforms from a single locus, the majority of loci generate only two or three isoforms and transcript support indicates that these isoforms are generally not rare events. For the alternate donor (AD and acceptor (AA classes, the distance between the splice sites for the majority of events was found to be less than 50 basepairs (bp. In both species, the most frequent distance between AA is 3 bp, consistent with reports in mammalian systems. Conversely, the most frequent distance between AD is 4 bp in both plant species, as previously observed in mouse. Most alternative splicing variations are localized to the protein coding sequence and are predicted to significantly alter the coding sequence. Conclusion Alternative splicing is widespread in both rice and Arabidopsis and these species share many common features. Interestingly, alternative splicing may play a role beyond creating novel combinations of transcripts that expand the proteome. Many isoforms will presumably have negative

  18. Genome-wide analysis of SRSF10-regulated alternative splicing by deep sequencing of chicken transcriptome

    Directory of Open Access Journals (Sweden)

    Xuexia Zhou

    2014-12-01

    Full Text Available Splicing factor SRSF10 is known to function as a sequence-specific splicing activator that is capable of regulating alternative splicing both in vitro and in vivo. We recently used an RNA-seq approach coupled with bioinformatics analysis to identify the extensive splicing network regulated by SRSF10 in chicken cells. We found that SRSF10 promoted both exon inclusion and exclusion. Functionally, many of the SRSF10-verified alternative exons are linked to pathways of response to external stimulus. Here we describe in detail the experimental design, bioinformatics analysis and GO/pathway enrichment analysis of SRSF10-regulated genes to correspond with our data in the Gene Expression Omnibus with accession number GSE53354. Our data thus provide a resource for studying regulation of alternative splicing in vivo that underlines biological functions of splicing regulatory proteins in cells.

  19. Alternative Splicing and Expression Profile Analysis of Expressed Sequence Tags in Domestic Pig

    Institute of Scientific and Technical Information of China (English)

    Liang Zhang; Lin Tao; Lin Ye; Ling He; Yuan-Zhong Zhu; Yue-Dong Zhu; Yan Zhou

    2007-01-01

    Domestic pig (Sus scrofa domestica) is one of the most important mammals to humans. Alternative splicing is a cellular mechanism in eukaryotes that greatly increases the diversity of gene products. Expression sequence tags (ESTs) have been widely used for gene discovery, expression profile analysis, and alternative splicing detection. In this study, a total of 712,905 ESTs extracted from 101 different nonnormalized EST libraries of the domestic pig were analyzed. These EST libraries cover the nervous system, digestive system, immune system, and meat production related tissues from embryo, newborn, and adult pigs, making contributions to the analysis of alternative splicing variants as well as expression profiles in various stages of tissues. A modified approach was designed to cluster and assemble large EST datasets, aiming to detect alternative splicing together with EST abundance of each splicing variant. Much efforts were made to classify alternative splicing into different types and apply different filters to each type to get more reliable results. Finally, a total of 1,223 genes with average 2.8 splicing variants were detected among 16,540 unique genes. The overview of expression profiles would change when we take alternative splicing into account.

  20. Method for exploiting bias in factor analysis using constrained alternating least squares algorithms

    Science.gov (United States)

    Keenan, Michael R.

    2008-12-30

    Bias plays an important role in factor analysis and is often implicitly made use of, for example, to constrain solutions to factors that conform to physical reality. However, when components are collinear, a large range of solutions may exist that satisfy the basic constraints and fit the data equally well. In such cases, the introduction of mathematical bias through the application of constraints may select solutions that are less than optimal. The biased alternating least squares algorithm of the present invention can offset mathematical bias introduced by constraints in the standard alternating least squares analysis to achieve factor solutions that are most consistent with physical reality. In addition, these methods can be used to explicitly exploit bias to provide alternative views and provide additional insights into spectral data sets.

  1. Demonstration of risk-based decision analysis in remedial alternative selection and design

    Energy Technology Data Exchange (ETDEWEB)

    Evans, E.K.; Duffield, G.M. (Geraghty and Miller Modeling Group, Reston, VA (United States)); Massmann, J.W. (Washington Univ., Seattle, WA (United States)); Freeze, R.A. (Freeze (R.A.) Engineering, Inc., White Rock, BC (Canada)); Stephenson, D.E. (Westinghouse Savannah River Co., Aiken, SC (United States))

    1993-01-01

    This study demonstrates the use of risk-based decision analysis (Massmann and Freeze 1987a, 1987b) in the selection and design of an engineering alternative for groundwater remediation at a waste site at the Savannah River Site, a US Department of Energy facility in South Carolina. The investigation focuses on the remediation and closure of the H-Area Seepage Basins, an inactive disposal site that formerly received effluent water from a nearby production facility. A previous study by Duffield et al. (1992), which used risk-based decision analysis to screen a number of ground-water remediation alternatives under consideration for this site, indicated that the most attractive remedial option is ground-water extraction by wells coupled with surface water discharge of treated effluent. The aim of the present study is to demonstrate the iterative use of risk-based decision analysis throughout the design of a particular remedial alternative. In this study, we consider the interaction between two episodes of aquifer testing over a 6-year period and the refinement of a remedial extraction well system design. Using a three-dimensional ground-water flow model, this study employs (1) geostatistics and Monte Carlo techniques to simulate hydraulic conductivity as a stochastic process and (2) Bayesian updating and conditional simulation to investigate multiple phases of aquifer testing. In our evaluation of a remedial alternative, we compute probabilistic costs associated with the failure of an alternative to completely capture a simulated contaminant plume. The results of this study demonstrate the utility of risk-based decision analysis as a tool for improving the design of a remedial alternative through the course of phased data collection at a remedial site.

  2. Demonstration of risk-based decision analysis in remedial alternative selection and design

    Energy Technology Data Exchange (ETDEWEB)

    Evans, E.K.; Duffield, G.M. [Geraghty and Miller Modeling Group, Reston, VA (United States); Massmann, J.W. [Washington Univ., Seattle, WA (United States); Freeze, R.A. [Freeze (R.A.) Engineering, Inc., White Rock, BC (Canada); Stephenson, D.E. [Westinghouse Savannah River Co., Aiken, SC (United States)

    1993-05-01

    This study demonstrates the use of risk-based decision analysis (Massmann and Freeze 1987a, 1987b) in the selection and design of an engineering alternative for groundwater remediation at a waste site at the Savannah River Site, a US Department of Energy facility in South Carolina. The investigation focuses on the remediation and closure of the H-Area Seepage Basins, an inactive disposal site that formerly received effluent water from a nearby production facility. A previous study by Duffield et al. (1992), which used risk-based decision analysis to screen a number of ground-water remediation alternatives under consideration for this site, indicated that the most attractive remedial option is ground-water extraction by wells coupled with surface water discharge of treated effluent. The aim of the present study is to demonstrate the iterative use of risk-based decision analysis throughout the design of a particular remedial alternative. In this study, we consider the interaction between two episodes of aquifer testing over a 6-year period and the refinement of a remedial extraction well system design. Using a three-dimensional ground-water flow model, this study employs (1) geostatistics and Monte Carlo techniques to simulate hydraulic conductivity as a stochastic process and (2) Bayesian updating and conditional simulation to investigate multiple phases of aquifer testing. In our evaluation of a remedial alternative, we compute probabilistic costs associated with the failure of an alternative to completely capture a simulated contaminant plume. The results of this study demonstrate the utility of risk-based decision analysis as a tool for improving the design of a remedial alternative through the course of phased data collection at a remedial site.

  3. Stiffness analysis and comparison of a Biglide parallel grinder with alternative spatial modular parallelograms

    DEFF Research Database (Denmark)

    Wu, Guanglei; Zou, Ping

    2016-01-01

    This paper deals with the stiffness modeling, analysis and comparison of a Biglide parallel grinder with two alternative modular parallelograms. It turns out that the Cartesian stiffness matrix of the manipulator has the property that it can be decoupled into two homogeneous matrices, correspondi...

  4. Energy alternatives for irrigation pumping: an economic analysis for northern India.

    OpenAIRE

    Bhatia R

    1984-01-01

    ILO pub-WEP pub. Working paper presenting an economic analysis of alternative energy sources for irrigation pumping in Northern India - considers economic and technical aspects of photovoltaic pumping systems, solar energy systems, electric power, dual-fuel and diesel engines, Biogas and wind power; discusses economic and social development aspects. Abbreviations, bibliography, glossary and tables.

  5. An Alternative Dimension Reduction Approach to Supervised Principal Components Analysis in High Dimensional Survival Data

    OpenAIRE

    Elvan AKTURK HAYAT; Mevlut TURE; Sansli SENOL

    2016-01-01

    Objective: This study aims at comparing the performances of supervised principal component analysis (SPCA) which is used for dimension reduction and an alternatively proposed approach of nonlinear principal component analysis using artificial neural networks performed by gene selection with survival tree (survival tree based NLPCA- NN). Material and Methods: Gene expression data set from Rosenwald et al.(2002) pertaining to 240 patients with diffuse large B-cell lymphoma (DLBCL) is used. Whil...

  6. SustainPro - A tool for systematic process analysis, generation and evaluation of sustainable design alternatives

    DEFF Research Database (Denmark)

    Carvalho, Ana; Matos, Henrique A.; Gani, Rafiqul

    2013-01-01

    the user through the necessary steps according to work-flow of the implemented methodology. At the end the design alternatives, are evaluated using environmental impact assessment tools and safety indices. The extended features of the methodology incorporate Life Cycle Assessment analysis and economic...... analysis. The application and the main features of SustainPro are illustrated through a case study of ß-Galactosidase production....

  7. An analysis of strategies used by chemistry instructors to address student alternate conceptions in chemical equilibrium

    Science.gov (United States)

    Piquette, Jeff Stephen

    This study explored general-chemistry instructors' awareness of and ability to identify common student alternate conceptions in chemical equilibrium. Instructor strategies directed at remediation of student alternate conceptions were also investigated and compared to successful, literature-based conceptual change methods. Fifty-two general chemistry instructor volunteers from 50 U.S. colleges and universities completed an interactive web-based survey that gathered their responses to open-ended questions, a rating scale, classroom scenarios, and a demographic form. The three scenarios asked respondents to evaluate hypothetical student exam answers, justify their evaluations, and report how they would assist students to better understand ideas about which they held alternate conceptions. Survey respondents who provided responses or remediation strategies that needed further clarification were sampled (n = 6); each amplified their views in an individual, researcher-led semi-structured phone interview. All survey responses and interview transcriptions were independently analyzed by three raters who followed Patton's (1990) guidelines for qualitative data analysis. Data analysis established that all 52 instructors of chemistry were able to report and identify common student alternate conceptions in chemical equilibrium. Those instructor-reported alternate conceptions were congruent with previously identified alternate conceptions (misconceptions) found in published literature, thus providing validation support for the earlier compilations. This study revealed that chemistry instructors employ a variety of strategies in efforts to address and remediate alternate conceptions. However, those strategies rarely include all four conditions outlined by Posner, Strike, Hewson, and Gertzog (1982) needed to stimulate conceptual change in students. Instructors are thus encouraged to become familiar with successful conceptual change strategies, using such methods as appropriate in

  8. Alternatives generation and analysis for the Phase I intermediate waste feed staging system design requirements

    Energy Technology Data Exchange (ETDEWEB)

    Claghorn, R.D., Fluor Daniel Hanford

    1997-02-06

    This alternatives generation and analysis (AGA) addresses the question: What is the design basis for the facilities required to stage low-level waste (LLW) feed to the Phase I private contractors? Alternative designs for the intermediate waste feed staging system were developed, analyzed, and compared. Based on these analyses, this document recommends installing mixer pumps in the central pump pit of double-shell tanks 241-AP-102 and 241-AP-104. Also recommended is installing decant/transfer pumps at these tanks. These recommendations have clear advantages in that they provide a low shedule impact/risk and the highest operability of all the alternatives investigated. This revision incorporates comments from the decision board.

  9. Design of sustainable chemical processes: Systematic retrofit analysis, generation and evaluation alternatives

    DEFF Research Database (Denmark)

    Carvalho, Ana; Gani, Rafiqul; Matos, Henrique

    2008-01-01

    -aided tool has been developed to facilitate the calculations needed for the application of the methodology. The application of the indicator-based methodology and the developed software are highlighted through a process flowsheet for the production of vinyl chlorine monomer (VCM).......The objective of this paper is to present a generic and systematic methodology for identifying the feasible retrofit design alternatives of any chemical process. The methodology determines a set of mass and energy indicators from steady-state process data, establishes the operational and design...... targets, and through a sensitivity-based analysis, identifies the design alternatives that can match a set of design targets. The significance of this indicator-based method is that it is able to identify alternatives, where one or more performance criteria (factors) move in the same direction thereby...

  10. Genome-Wide Analysis of Alternative Splicing during Development and Drought Stress in Maize.

    Science.gov (United States)

    Thatcher, Shawn R; Danilevskaya, Olga N; Meng, Xin; Beatty, Mary; Zastrow-Hayes, Gina; Harris, Charlotte; Van Allen, Brandon; Habben, Jeffrey; Li, Bailin

    2016-01-01

    Alternative splicing plays a crucial role in plant development as well as stress responses. Although alternative splicing has been studied during development and in response to stress, the interplay between these two factors remains an open question. To assess the effects of drought stress on developmentally regulated splicing in maize (Zea mays), 94 RNA-seq libraries from ear, tassel, and leaf of the B73 public inbred line were constructed at four developmental stages under both well-watered and drought conditions. This analysis was supplemented with a publicly available series of 53 libraries from developing seed, embryo, and endosperm. More than 48,000 novel isoforms, often with stage- or condition-specific expression, were uncovered, suggesting that developmentally regulated alternative splicing occurs in thousands of genes. Drought induced large developmental splicing changes in leaf and ear but relatively few in tassel. Most developmental stage-specific splicing changes affected by drought were tissue dependent, whereas stage-independent changes frequently overlapped between leaf and ear. A linear relationship was found between gene expression changes in splicing factors and alternative spicing of other genes during development. Collectively, these results demonstrate that alternative splicing is strongly associated with tissue type, developmental stage, and stress condition.

  11. Genome-Wide Analysis of Alternative Splicing during Development and Drought Stress in Maize1[OPEN

    Science.gov (United States)

    Thatcher, Shawn R.; Meng, Xin; Beatty, Mary; Zastrow-Hayes, Gina; Harris, Charlotte; Habben, Jeffrey; Li, Bailin

    2016-01-01

    Alternative splicing plays a crucial role in plant development as well as stress responses. Although alternative splicing has been studied during development and in response to stress, the interplay between these two factors remains an open question. To assess the effects of drought stress on developmentally regulated splicing in maize (Zea mays), 94 RNA-seq libraries from ear, tassel, and leaf of the B73 public inbred line were constructed at four developmental stages under both well-watered and drought conditions. This analysis was supplemented with a publicly available series of 53 libraries from developing seed, embryo, and endosperm. More than 48,000 novel isoforms, often with stage- or condition-specific expression, were uncovered, suggesting that developmentally regulated alternative splicing occurs in thousands of genes. Drought induced large developmental splicing changes in leaf and ear but relatively few in tassel. Most developmental stage-specific splicing changes affected by drought were tissue dependent, whereas stage-independent changes frequently overlapped between leaf and ear. A linear relationship was found between gene expression changes in splicing factors and alternative spicing of other genes during development. Collectively, these results demonstrate that alternative splicing is strongly associated with tissue type, developmental stage, and stress condition. PMID:26582726

  12. Evaluation of alternative tissues and equipment for the analysis of neutral and acid detergente fiber.

    Directory of Open Access Journals (Sweden)

    Juliana Santos Farias

    2015-10-01

    Full Text Available The objective of this study was to compare alternative devices and tissues for the determination of neutral detergent fiber (NDF and acid detergent fiber (ADF with the conventional method. Two tissues, nylon (50 μm and TNT (100 g/m², and two devices (fiber digester and autoclave were used for the determination of NDF and ADF content compared to the conventional method. A completely randomized experimental design, which consisted of five treatments and 10 repetitions, was used. The NDF content obtained with TNT in the two devices differed from that obtained with the conventional method (P<0.01. However, the use of nylon in the autoclave and fiber digester provided similar values (P>0.01, indicating this combination as a possible alternative to the conventional method. There was no difference (P>0.01 between the alternative methods for the analysis of NDF. The content of ADF differed between the devices and tissues evaluated (P<0.01. However, the use of nylon tissue in the fiber digester provided values similar to the conventional method (P>0.01 and may be indicated as a practical alternative for the analysis of ADF. The NDF content obtained with nylon tissue, irrespective of the equipment used, and the ADF content obtained with nylon tissue in the fiber digester were similar to the conventional method.

  13. Advanced analysis technique for the evaluation of linear alternators and linear motors

    Science.gov (United States)

    Holliday, Jeffrey C.

    1995-01-01

    A method for the mathematical analysis of linear alternator and linear motor devices and designs is described, and an example of its use is included. The technique seeks to surpass other methods of analysis by including more rigorous treatment of phenomena normally omitted or coarsely approximated such as eddy braking, non-linear material properties, and power losses generated within structures surrounding the device. The technique is broadly applicable to linear alternators and linear motors involving iron yoke structures and moving permanent magnets. The technique involves the application of Amperian current equivalents to the modeling of the moving permanent magnet components within a finite element formulation. The resulting steady state and transient mode field solutions can simultaneously account for the moving and static field sources within and around the device.

  14. Life Cycle Multi-Criteria Analysis Of Alternative Energy Supply Systems For A Residential Building

    Directory of Open Access Journals (Sweden)

    Artur Rogoža

    2013-12-01

    Full Text Available The article analyses energy supply alternatives for a partially renovated residential building. In addition to the existing district heating (base case alternative systems, gas boilers, heat pumps (air-water and ground-water, solar collectors, solar cells, and combinations of these systems have been examined. Actual heat consumption of the building and electricity demand determined by the statistical method are used for simulating the systems. The process of simulation is performed using EnergyPro software. In order to select an optimal energy supply option, the life cycle analysis of all systems has been carried out throughout a life span of the building, and the estimated results of energy, environmental and economic evaluation have been converted into non-dimensional variables (3E using multi–criteria analysis.Article in Lithuanian

  15. Cluster analysis in kinetic modelling of the brain: A noninvasive alternative to arterial sampling

    DEFF Research Database (Denmark)

    Liptrot, Matthew George; Adams, K.H.; Martiny, L.;

    2004-01-01

    In emission tomography, quantification of brain tracer uptake, metabolism or binding requires knowledge of the cerebral input function. Traditionally, this is achieved with arterial blood sampling. We propose a noninvasive alternative via the use of a blood vessel time-activity curve (TAC......) extracted directly from dynamic positron emission tomography (PET) scans by cluster analysis. Five healthy subjects were injected with the 5HT2A- receptor ligand [18F]-altanserin and blood samples were subsequently taken from the radial artery and cubital vein. Eight regions-of-interest (ROI) TACs were...... by the 'within-variance' measure and by 3D visual inspection of the homogeneity of the determined clusters. The cluster-determined input curve was then used in Logan plot analysis and compared with the arterial and venous blood samples, and additionally with one of the currently used alternatives to arterial...

  16. Functional communication training with and without alternative reinforcement and punishment: an analysis of 58 applications.

    Science.gov (United States)

    Rooker, Griffin W; Jessel, Joshua; Kurtz, Patricia F; Hagopian, Louis P

    2013-12-01

    Functional communication training (FCT) is an empirically supported treatment for problem behavior displayed by individuals with intellectual disabilities. Hagopian, Fisher, Sullivan, Acquisto, and LeBlanc (1998) analyzed 25 applications of FCT and showed that extinction was a necessary component of FCT, but sometimes punishment was needed to maintain low levels of problem behavior. The current consecutive case series summarized data from 58 applications of FCT in more recent cases. This analysis extended and updated Hagopian et al. by examining FCT when used in combination with alternative reinforcement (noncontingent and differential reinforcement) and multiple schedules during schedule thinning. Although it is difficult to make direct comparisons with the 1998 study, the results of the current case series analysis suggest that FCT can be enhanced when used in combination with alternative reinforcement and when multiple schedules are used during schedule thinning.

  17. Functional communication training with and without alternative reinforcement and punishment: an analysis of 58 applications.

    Science.gov (United States)

    Rooker, Griffin W; Jessel, Joshua; Kurtz, Patricia F; Hagopian, Louis P

    2013-12-01

    Functional communication training (FCT) is an empirically supported treatment for problem behavior displayed by individuals with intellectual disabilities. Hagopian, Fisher, Sullivan, Acquisto, and LeBlanc (1998) analyzed 25 applications of FCT and showed that extinction was a necessary component of FCT, but sometimes punishment was needed to maintain low levels of problem behavior. The current consecutive case series summarized data from 58 applications of FCT in more recent cases. This analysis extended and updated Hagopian et al. by examining FCT when used in combination with alternative reinforcement (noncontingent and differential reinforcement) and multiple schedules during schedule thinning. Although it is difficult to make direct comparisons with the 1998 study, the results of the current case series analysis suggest that FCT can be enhanced when used in combination with alternative reinforcement and when multiple schedules are used during schedule thinning. PMID:24114463

  18. A brief functional analysis of aggressive and alternative behavior in an outclinic setting.

    OpenAIRE

    Northup, J; Wacker, D; Sasso, G; Steege, M; Cigrand, K; Cook, J; DeRaad, A

    1991-01-01

    We conducted a brief functional analysis to identify maintaining variable for aggressive behavior and an alternative replacement response during a 90-min outpatient evaluation of 3 individuals with severe handicaps. During the initial analogue assessment, which focused on identifying maintaining contingencies for aggressive behavior, each participant displayed a substantially greater frequency of aggressive behavior during one condition than during any other. The contingency that produced the...

  19. A Statistical Analysis of the Nominative/Accusative Alternation in Japanese : A Preliminary Study

    OpenAIRE

    牧, 秀樹; 森島, 玉峰; マキ, ヒデキ; モリシマ, タマミ; Maki, Hideki; MORISHIMA, Tamami

    2004-01-01

    This paper investigates the Nominative/Accusative alternation exhibited by Japanese stative predicates, which in principle take a Nominative Object. Through a statistical analysis, we found two important facts : (I) that when an Accusative Object is moved to the sentence initial position, some types of stative predicates allow an Accusative Object, and (2) that when the sentence containing a stative predicate and an Accusative Object is embedded into a complex NP, all types of stative predica...

  20. Artificial Neural Network Analysis of Sierpinski Gasket Fractal Antenna: A Low Cost Alternative to Experimentation

    Directory of Open Access Journals (Sweden)

    Balwinder S. Dhaliwal

    2013-01-01

    Full Text Available Artificial neural networks due to their general-purpose nature are used to solve problems in diverse fields. Artificial neural networks (ANNs are very useful for fractal antenna analysis as the development of mathematical models of such antennas is very difficult due to complex shapes and geometries. As such empirical approach doing experiments is costly and time consuming, in this paper, application of artificial neural networks analysis is presented taking the Sierpinski gasket fractal antenna as an example. The performance of three different types of networks is evaluated and the best network for this type of applications has been proposed. The comparison of ANN results with experimental results validates that this technique is an alternative to experimental analysis. This low cost method of antenna analysis will be very useful to understand various aspects of fractal antennas.

  1. A Methodology for the Analysis and Selection of Alternative for the Disposition of Surplus Plutonium

    International Nuclear Information System (INIS)

    The Department of Energy (DOE) - Office of Fissile Materials Disposition (OFMD) has announced a Record of Decision (ROD) selecting alternatives for disposition of surplus plutonium. A major objective of this decision was to further U.S. efforts to prevent the proliferation of nuclear weapons. Other concerns that were addressed include economic, technical, institutional, schedule, environmental, and health and safety issues. The technical, environmental, and nonproliferation analyses supporting the ROD are documented in three DOE reports (DOE-TSR 96, DOE-PEIS 96, and DOE-NN 97, respectively). At the request of OFMD, a team of analysts from the Amarillo National Resource Center for Plutonium (ANRCP) provided an independent evaluation of the alternatives for plutonium that were considered during the evaluation effort. This report outlines the methodology used by the ANRCP team. This methodology, referred to as multiattribute utility theory (MAU), provides a structure for assembling results of detailed technical, economic, schedule, environment, and nonproliferation analyses for OFMD, DOE policy makers, other stakeholders, and the general public in a systematic way. The MAU methodology has been supported for use in similar situations by the National Research Council, an agency of the National Academy of Sciences.1 It is important to emphasize that the MAU process does not lead to a computerized model that actually determines the decision for a complex problem. MAU is a management tool that is one component, albeit a key component, of a decision process. We subscribe to the philosophy that the result of using models should be insights, not numbers. The MAU approach consists of four steps: (1) identification of alternatives, objectives, and performance measures, (2) estimation of the performance of the alternatives with respect to the objectives, (3) development of value functions and weights for the objectives, and (4) evaluation of the alternatives and sensitivity

  2. FUS-mediated regulation of alternative RNA processing in neurons: insights from global transcriptome analysis.

    Science.gov (United States)

    Masuda, Akio; Takeda, Jun-Ichi; Ohno, Kinji

    2016-05-01

    Fused in sarcoma (FUS) is an RNA-binding protein that is causally associated with oncogenesis and neurodegeneration. Recently, the role of FUS in neurodegeneration has been extensively studied, because mutations in FUS are associated with amyotrophic lateral sclerosis (ALS), and the FUS protein has been identified as a major component of intracellular inclusions in neurodegenerative disorders including ALS and frontotemporal lobar degeneration. FUS is a key molecule in transcriptional regulation and RNA processing including processes such as pre-messenger RNA (mRNA) splicing and polyadenylation. Interaction of FUS with various components of the transcription machinery, spliceosome, and the 3'-end processing machinery has been identified. Furthermore, recent advances in high-throughput transcriptomic profiling approaches have enabled us to determine the mechanisms of FUS-dependent RNA processing networks at a cellular level. These analyses have revealed that depletion of FUS in neuronal cells affects alternative splicing and alternative polyadenylation of thousands of mRNAs. Gene ontology analysis has suggested that FUS-modulated genes are implicated in neuronal functions and development. CLIP-seq of FUS has shown that FUS is frequently clustered around these alternative sites of nascent RNA. ChIP-seq of RNA polymerase II (RNAP II) has demonstrated that an interaction between FUS and nascent RNA downregulates local transcriptional activity of RNAP II, which is critically involved in RNA processing. Both alternative splicing and alternative polyadenylation are fundamental processes by which cells expand their transcriptomic diversity, and are particularly essential in the nervous system. Dependence of transcriptomic diversity on FUS makes the nervous system vulnerable to neurodegeneration, when FUS is functionally compromised. WIREs RNA 2016, 7:330-340. doi: 10.1002/wrna.1338 For further resources related to this article, please visit the WIREs website. PMID:26822113

  3. Learning Bayesian networks from big meteorological spatial datasets. An alternative to complex network analysis

    Science.gov (United States)

    Gutiérrez, Jose Manuel; San Martín, Daniel; Herrera, Sixto; Santiago Cofiño, Antonio

    2016-04-01

    The growing availability of spatial datasets (observations, reanalysis, and regional and global climate models) demands efficient multivariate spatial modeling techniques for many problems of interest (e.g. teleconnection analysis, multi-site downscaling, etc.). Complex networks have been recently applied in this context using graphs built from pairwise correlations between the different stations (or grid boxes) forming the dataset. However, this analysis does not take into account the full dependence structure underlying the data, gien by all possible marginal and conditional dependencies among the stations, and does not allow a probabilistic analysis of the dataset. In this talk we introduce Bayesian networks as an alternative multivariate analysis and modeling data-driven technique which allows building a joint probability distribution of the stations including all relevant dependencies in the dataset. Bayesian networks is a sound machine learning technique using a graph to 1) encode the main dependencies among the variables and 2) to obtain a factorization of the joint probability distribution of the stations given by a reduced number of parameters. For a particular problem, the resulting graph provides a qualitative analysis of the spatial relationships in the dataset (alternative to complex network analysis), and the resulting model allows for a probabilistic analysis of the dataset. Bayesian networks have been widely applied in many fields, but their use in climate problems is hampered by the large number of variables (stations) involved in this field, since the complexity of the existing algorithms to learn from data the graphical structure grows nonlinearly with the number of variables. In this contribution we present a modified local learning algorithm for Bayesian networks adapted to this problem, which allows inferring the graphical structure for thousands of stations (from observations) and/or gridboxes (from model simulations) thus providing new

  4. Systemic Functional Linguistics and Discourse Analysis as Alternatives When Dealing With Texts

    Directory of Open Access Journals (Sweden)

    Paula Andrea García Montes

    2014-10-01

    Full Text Available This article presents a case study research with aims to find out which activities, methodological and textual aspects used in a reading strategies course were causing a group of students difficulties when analyzing critically written information. We conducted the study at Universidad de Córdoba (Colombia with seventh semester students from the undergraduate English program. The data collection techniques used included observations, document analysis, interviews, and a Preliminary English Test for students. The results were analyzed taking into account issues in discourse analysis and systemic functional linguistics. Results show reasons why students find it difficult to successfully complete the analysis of written texts. These results can serve as useful alternatives for planning reading lessons.

  5. Evaluation of alternatives for microalgae oil extraction based on exergy analysis

    International Nuclear Information System (INIS)

    Highlights: ► Exergy analysis was used as decision-making tool for evaluation of microalgae oil extraction. ► A robust composition of Chlorella sp. biomass was modeled and used for simulation. ► Three solvent-based microalgae oil extraction methods at large scale were compared. ► Hexane based extraction presented the highest exergetic efficiency. -- Abstract: Several technologies for microalgae oil extraction are being evaluated in order to find the most adequate for large scale microalgae processing. In this work, exergy analysis was used as an instrument for screening three design alternatives for microalgae oil extraction in a large-scale process and as a decision-making tool for evaluation and selection of novel technologies from the energy point of view. Routes were simulated using dedicated industrial process simulation software, taking as feedstock a representative and robust modeled composition of Chlorella sp. microalgae biomass. Mass, energy and exergy balances were performed for each alternative, and physical and chemical exergies of streams and all specific microalgae constituents modeled were calculated with the help of the thermodynamic properties of biomass components and operating conditions of streams. Exergetic efficiencies, total process irreversibilities, energy consumption and exergy destruction were calculated for all solvent-based microalgae oil extraction pathways evaluated. It was shown that exergy analysis led to identify the hexane-based oil extraction (HBE) as the most adequate alternative of the routes assessed for scaling up from the energy point of view, presenting a maximum exergy efficiency of 51% and exergetic losses of 982,000 MJ considering a production of 104,000 t of microalgae oil per year.

  6. Stability Analysis of Ranking Alternatives Based on Subjective and Objective Weight

    Institute of Scientific and Technical Information of China (English)

    JIANG Yan; ZUO Bao-he; YUE Chao-yuan

    2002-01-01

    Weights of criteria are used to assess the relative importance of the different criteria in multicriteria analysis, which can influence ranking result more or less depending on the multicriteria decisionmaking method used. In this paper, the influences of alternatives' ranking result associated with the change of weight are discussed by making use of the concept of weight stability intervals based on subjective and objective integrated weighting approach. Meamwhile, A model of weight proportion stability intervals is proposed. a numeral example is used to illuminate how many increment of objective weight can change the ranking results determined by subjective weight.

  7. The Analysis of Fisher Hypothesis in Terms of Turkish Economy Through Alternative Interest Rates

    Directory of Open Access Journals (Sweden)

    Fuat Lebe

    2016-06-01

    Full Text Available The purpose of this study is to analyze the relationship between the interest rate and inflation rate in the context of Fisher hypothesis (1930 by using the data regarding Turkish Economy Differing from the available literature, the validity of Fisher hypothesis for Turkey was assessed according to the alternative interest rates (rediscount interest rate, deposit interest rate and the monetary policy interest rate. The long-term relationship among variables was analyzed via ARDL bound test. As a result of this analysis, it was determined that a stronger Fisher impact is valid for Turkish economy when the monetary interest rate is based as interest rate.

  8. Genome-wide analysis of alternative transcripts in human breast cancer

    Science.gov (United States)

    Wen, Ji; Toomer, Kevin H.

    2016-01-01

    Transcript variants play a critical role in diversifying gene expression. Alternative splicing is a major mechanism for generating transcript variants. A number of genes have been implicated in breast cancer pathogenesis with their aberrant expression of alternative transcripts. In this study, we performed genome-wide analyses of transcript variant expression in breast cancer. With RNA-Seq data from 105 patients, we characterized the transcriptome of breast tumors, by pairwise comparison of gene expression in the breast tumor versus matched healthy tissue from each patient. We identified 2839 genes, ~10 % of protein-coding genes in the human genome, that had differential expression of transcript variants between tumors and healthy tissues. The validity of the computational analysis was confirmed by quantitative RT-PCR assessment of transcript variant expression from four top candidate genes. The alternative transcript profiling led to classification of breast cancer into two subgroups and yielded a novel molecular signature that could be prognostic of patients’ tumor burden and survival. We uncovered nine splicing factors (FOX2, MBNL1, QKI, PTBP1, ELAVL1, HNRNPC, KHDRBS1, SFRS2, and TIAR) that were involved in aberrant splicing in breast cancer. Network analyses for the coordinative patterns of transcript variant expression identified twelve “hub” genes that differentiated the cancerous and normal transcriptomes. Dysregulated expression of alternative transcripts may reveal novel biomarkers for tumor development. It may also suggest new therapeutic targets, such as the “hub” genes identified through the network analyses of transcript variant expression, or splicing factors implicated in the formation of the tumor transcriptome. PMID:25913416

  9. Radiological impacts of Jackpile-Paguate uranium mines: an analysis of alternatives of decommissioning

    International Nuclear Information System (INIS)

    Potential pathways of radiation exposure and radiation-induced genetic and somatic effects from materials at the mine complex under five alternatives of decommissioning were analyzed using UDAD and PRIM computer codes. The principal pathways of exposure included in the analysis were inhalation of airborne radionuclides, ingestion of food and water containing radionuclides, and extended exposure to gamma and beta radiation from either airborne or ground-deposited radionuclides. The alternatives of decommissioning include (A) No Action (site will be fenced, otherwise left as it is), (B) No Future Use (site will be fenced and all disturbed area will be covered with 30 cm of soil, no grazing on the site); (C1) Grazing Land Use as developed by Anaconda Company (protore, waste piles, and open pits covered with 120 cm of soil, the remainder of the disturbed areas covered with 30 cm of soil, pits backfilled 90 cm above the equilibrium groundwater recovery level, no human habitation or farming allowed on the mine site, but grazing would be allowed); (C2) Grazing Land Use as developed by US Department of the Interior (similar to Alternative C1, but the pits covered with 300 cm of soil above the groundwater recovery level); and (D) Maximum Future Use (similar to Alternative C2, except construction of commercial and industrial facilities, storage, recreation, and further mining would be allowed). Radiation doses from atmospheric transport and ingestion of radionuclides were calculated, and somatic and genetic effects in individuals living within 80 km from the mine complex were predicted. Hydrological flow patterns in the mine area were analyzed to determine the potential for future contamination of surface water and groundwater and to determine the groundwater recovery level after reclamation, thus permitting incorporation of corrective actions into the reclamation procedures

  10. Spectral analysis of bilateral or alternate-site kindling-induced afterdischarges in the rabbit hippocampi.

    Science.gov (United States)

    Tsuchiya, Komei; Kogure, Shinichi

    2012-09-01

    Kindling is one of the popular animal models of temporal lobe epilepsy. In the present study following the previous results obtained using unilateral hippocampal kindling (UK), we performed spectral analysis of bilateral or alternate-site kindling-induced afterdischarges (ADs) in the rabbit hippocampi. Eight and ten adult rabbits were used for bilateral kindling (BK) and alternate-site kindling (AK), respectively. Kindling stimuli consisted of a train of biphasic pulses (1ms duration each) of 50Hz for 1s, with suprathreshold intensity for AD. The stimulations were applied simultaneously to the bilateral hippocampi in the BK and were delivered to the right and left hippocampus once every 24h in the AK. Motor responses were classified into five stages according to the conventional criteria. All animals in BK as well as AK developed stage 5 convulsions. This contrasts to the result of UK (kindled: 50%; incomplete: 50%). We normalized power spectral density (PSD) and monitored the changes in the proportion of lower frequency band component (LFB: 0-9Hz) and the higher frequency band (HFB: 12-30Hz). BK animals showed a significantly large decrement (0.5 times, pkindling progression. Very strong positive correlations were found in both kindling animals. Chronological spectral analysis of seizure discharges, resulting in a pattern of LFB decrement accompanied by HFB increment, is a convenient tool to investigate epileptic disorders and diagnose epileptic states.

  11. Comparative life-cycle cost analysis for low-level mixed waste remediation alternatives

    International Nuclear Information System (INIS)

    The purpose of this study is two-fold: (1) to develop a generic, life-cycle cost model for evaluating low-level, mixed waste remediation alternatives, and (2) to apply the model specifically, to estimate remediation costs for a site similar to the Fernald Environmental Management Project near Cincinnati, OH. Life-cycle costs for vitrification, cementation, and dry removal process technologies are estimated. Since vitrification is in a conceptual phase, computer simulation is used to help characterize the support infrastructure of a large scale vitrification plant. Cost estimating relationships obtained from the simulation data, previous cost estimates, available process data, engineering judgment, and expert opinion all provide input to an Excel based spreadsheet for generating cash flow streams. Crystal Ball, an Excel add-on, was used for discounting cash flows for net present value analysis. The resulting LCC data was then analyzed using multi-attribute decision analysis techniques with cost and remediation time as criteria. The analytical framework presented allows alternatives to be evaluated in the context of budgetary, social, and political considerations. In general, the longer the remediation takes, the lower the net present value of the process. This is true because of the time value of money and large percentage of the costs attributed to storage or disposal

  12. Frequency-based image analysis of random patterns: an alternative way to classical stereocorrelation

    CERN Document Server

    Molimard, Jérôme; Zahouani, Hassan

    2013-01-01

    The paper presents an alternative way to classical stereocorrelation. First, 2D image processing of random patterns is described. Sub-pixel displacements are determined using phase analysis. Then distortion evaluation is presented. The distortion is identified without any assumption on the lens model because of the use of a grid technique approach. Last, shape measurement and shape variation is caught by fringe projection. Analysis is based on two pin-hole assumptions for the video-projector and the camera. Then, fringe projection is coupled to in-plane displacement to give rise to 3D measurement set-up. Metrological characterization shows a resolution comparable to classical (stereo) correlation technique (1/100th pixel). Spatial resolution seems to be an advantage of the method, because of the use of temporal phase stepping (shape measurement, 1 pixel) and windowed Fourier transform (in plane displacements measurement, 9 pixels). Two examples are given. First one is the study of skin properties; second one ...

  13. Towards for Analyzing Alternatives of Interaction Design Based on Verbal Decision Analysis of User Experience

    Directory of Open Access Journals (Sweden)

    Marília Soares Mendes

    2010-04-01

    Full Text Available In domains (as digital TV, smart home, and tangible interfaces that represent a new paradigm of interactivity, the decision of the most appropriate interaction design solution is a challenge. HCI researchers have promoted in their works the validation of design alternative solutions with users before producing the final solution. User experience with technology is a subject that has also gained ground in these works in order to analyze the appropriate solution(s. Following this concept, a study was accomplished under the objective of finding a better interaction solution for an application of mobile TV. Three executable applications of mobile TV prototypes were built. A Verbal Decision Analysis model was applied on the investigations for the favorite characteristics in each prototype based on the user’s experience and their intentions of use. This model led a performance of a qualitative analysis which objectified the design of a new prototype.

  14. Alternative model for assessment administration and analysis: An example from the E-CLASS

    CERN Document Server

    Wilcox, Bethany R; Hobbs, Robert D; Aiken, John M; Welch, Nathan M; Lewandowski, H J

    2016-01-01

    The primary model for dissemination of conceptual and attitudinal assessments that has been used within the physics education research (PER) community is to create a high quality, validated assessment, make it available for a wide range of instructors to use, and provide minimal (if any) support to assist with administration or analysis of the results. Here, we present and discuss an alternative model for assessment dissemination, which is characterized by centralized data collection and analysis. This model also provides a greater degree of support for both researchers and instructors. Specifically, we describe our experiences developing a centralized, automated system for an attitudinal assessment we previously created to examine students' epistemologies and expectations about experimental physics. This system provides a proof-of-concept that we use to discuss the advantages associated with centralized administration and data collection for research-based assessments in PER. We also discuss the challenges t...

  15. Strategies for cost-effective carbon reductions: A sensitivity analysis of alternative scenarios

    Energy Technology Data Exchange (ETDEWEB)

    Gumerman, Etan; Koomey, Jonathan G.; Brown, Marilyn

    2001-07-11

    Analyses of alternative futures often present results for a limited set of scenarios, with little if any sensitivity analysis to identify the factors affecting the scenario results. This approach creates an artificial impression of certainty associated with the scenarios considered, and inhibits understanding of the underlying forces. This paper summarizes the economic and carbon savings sensitivity analysis completed for the Scenarios for a Clean Energy Future study (IWG, 2000). Its 19 sensitivity cases provide insight into the costs and carbon-reduction impacts of a carbon permit trading system, demand-side efficiency programs, and supply-side policies. Impacts under different natural gas and oil price trajectories are also examined. The results provide compelling evidence that policy opportunities exist to reduce carbon emissions and save society money.

  16. Confirmatory Factor Analysis of the Structure of Statistics Anxiety Measure: An Examination of Four Alternative Models

    Directory of Open Access Journals (Sweden)

    Hossein Bevrani, PhD

    2011-09-01

    Full Text Available Objective: The aim of this study is to explore the confirmatory factor analysis results of the Persian adaptation of Statistics Anxiety Measure (SAM, proposed by Earp.Method: The validity and reliability assessments of the scale were performed on 298 college students chosen randomly from Tabriz University in Iran. Confirmatory factor analysis (CFA was carried out to determine the factor structures of the Persian adaptation of SAM.Results: As expected, the second order model provided a better fit to the data than the three alternative models. Conclusions: Hence, SAM provides an equally valid measure for use among college students. The study both expands and adds support to the existing body of math anxiety literature.

  17. Alternative model for administration and analysis of research-based assessments

    Science.gov (United States)

    Wilcox, Bethany R.; Zwickl, Benjamin M.; Hobbs, Robert D.; Aiken, John M.; Welch, Nathan M.; Lewandowski, H. J.

    2016-06-01

    Research-based assessments represent a valuable tool for both instructors and researchers interested in improving undergraduate physics education. However, the historical model for disseminating and propagating conceptual and attitudinal assessments developed by the physics education research (PER) community has not resulted in widespread adoption of these assessments within the broader community of physics instructors. Within this historical model, assessment developers create high quality, validated assessments, make them available for a wide range of instructors to use, and provide minimal (if any) support to assist with administration or analysis of the results. Here, we present and discuss an alternative model for assessment dissemination, which is characterized by centralized data collection and analysis. This model provides a greater degree of support for both researchers and instructors in order to more explicitly support adoption of research-based assessments. Specifically, we describe our experiences developing a centralized, automated system for an attitudinal assessment we previously created to examine students' epistemologies and expectations about experimental physics. This system provides a proof of concept that we use to discuss the advantages associated with centralized administration and data collection for research-based assessments in PER. We also discuss the challenges that we encountered while developing, maintaining, and automating this system. Ultimately, we argue that centralized administration and data collection for standardized assessments is a viable and potentially advantageous alternative to the default model characterized by decentralized administration and analysis. Moreover, with the help of online administration and automation, this model can support the long-term sustainability of centralized assessment systems.

  18. Genome-wide analysis of alternative splicing during human heart development

    Science.gov (United States)

    Wang, He; Chen, Yanmei; Li, Xinzhong; Chen, Guojun; Zhong, Lintao; Chen, Gangbing; Liao, Yulin; Liao, Wangjun; Bin, Jianping

    2016-01-01

    Alternative splicing (AS) drives determinative changes during mouse heart development. Recent high-throughput technological advancements have facilitated genome-wide AS, while its analysis in human foetal heart transition to the adult stage has not been reported. Here, we present a high-resolution global analysis of AS transitions between human foetal and adult hearts. RNA-sequencing data showed extensive AS transitions occurred between human foetal and adult hearts, and AS events occurred more frequently in protein-coding genes than in long non-coding RNA (lncRNA). A significant difference of AS patterns was found between foetal and adult hearts. The predicted difference in AS events was further confirmed using quantitative reverse transcription-polymerase chain reaction analysis of human heart samples. Functional foetal-specific AS event analysis showed enrichment associated with cell proliferation-related pathways including cell cycle, whereas adult-specific AS events were associated with protein synthesis. Furthermore, 42.6% of foetal-specific AS events showed significant changes in gene expression levels between foetal and adult hearts. Genes exhibiting both foetal-specific AS and differential expression were highly enriched in cell cycle-associated functions. In conclusion, we provided a genome-wide profiling of AS transitions between foetal and adult hearts and proposed that AS transitions and deferential gene expression may play determinative roles in human heart development. PMID:27752099

  19. Bayesian Factor Analysis as a Variable-Selection Problem: Alternative Priors and Consequences.

    Science.gov (United States)

    Lu, Zhao-Hua; Chow, Sy-Miin; Loken, Eric

    2016-01-01

    Factor analysis is a popular statistical technique for multivariate data analysis. Developments in the structural equation modeling framework have enabled the use of hybrid confirmatory/exploratory approaches in which factor-loading structures can be explored relatively flexibly within a confirmatory factor analysis (CFA) framework. Recently, Muthén & Asparouhov proposed a Bayesian structural equation modeling (BSEM) approach to explore the presence of cross loadings in CFA models. We show that the issue of determining factor-loading patterns may be formulated as a Bayesian variable selection problem in which Muthén and Asparouhov's approach can be regarded as a BSEM approach with ridge regression prior (BSEM-RP). We propose another Bayesian approach, denoted herein as the Bayesian structural equation modeling with spike-and-slab prior (BSEM-SSP), which serves as a one-stage alternative to the BSEM-RP. We review the theoretical advantages and disadvantages of both approaches and compare their empirical performance relative to two modification indices-based approaches and exploratory factor analysis with target rotation. A teacher stress scale data set is used to demonstrate our approach. PMID:27314566

  20. Analysis of Alternative Refrigerants to R22 for Air-Conditioning Applications at Various Evaporating Temperatures

    Directory of Open Access Journals (Sweden)

    S. Venkataiah

    2014-03-01

    Full Text Available This paper presents the simulation results of a 1.5 ton capacity room air conditioning system with some selected refrigerants that have been assessed for their suitability as alternative refrigerants to R22 for air conditioning applications. The refrigerants with zero Ozone depletion potential only are selected in this study. The performance of selected refrigerants viz, R22, R134a, R404A, R407C, R410A, R507A, R290 and R600a is considered in the present analysis. The thermodynamic analysis of these refrigerants has been carried out on these selected refrigerants using COOLPACK software. The analysis mainly focuses on obtaining results of parameters with fixed condenser temperature but with variable evaporator temperatures. The parameters like heat rejection rate, mass flow rate of refrigerant, displacement volume, power input, discharge temperature, cop, saturation pressure and pressures ratio are analyzed. The thermodynamic analysis of eight selected refrigerants is carried out using the simulation software COOL PACK version 1.49 and a comparative study of the different refrigerants is made.

  1. Bayesian Factor Analysis as a Variable-Selection Problem: Alternative Priors and Consequences.

    Science.gov (United States)

    Lu, Zhao-Hua; Chow, Sy-Miin; Loken, Eric

    2016-01-01

    Factor analysis is a popular statistical technique for multivariate data analysis. Developments in the structural equation modeling framework have enabled the use of hybrid confirmatory/exploratory approaches in which factor-loading structures can be explored relatively flexibly within a confirmatory factor analysis (CFA) framework. Recently, Muthén & Asparouhov proposed a Bayesian structural equation modeling (BSEM) approach to explore the presence of cross loadings in CFA models. We show that the issue of determining factor-loading patterns may be formulated as a Bayesian variable selection problem in which Muthén and Asparouhov's approach can be regarded as a BSEM approach with ridge regression prior (BSEM-RP). We propose another Bayesian approach, denoted herein as the Bayesian structural equation modeling with spike-and-slab prior (BSEM-SSP), which serves as a one-stage alternative to the BSEM-RP. We review the theoretical advantages and disadvantages of both approaches and compare their empirical performance relative to two modification indices-based approaches and exploratory factor analysis with target rotation. A teacher stress scale data set is used to demonstrate our approach.

  2. Using soil function evaluation in multi-criteria decision analysis for sustainability appraisal of remediation alternatives.

    Science.gov (United States)

    Volchko, Yevheniya; Norrman, Jenny; Rosén, Lars; Bergknut, Magnus; Josefsson, Sarah; Söderqvist, Tore; Norberg, Tommy; Wiberg, Karin; Tysklind, Mats

    2014-07-01

    Soil contamination is one of the major threats constraining proper functioning of the soil and thus provision of ecosystem services. Remedial actions typically only address the chemical soil quality by reducing total contaminant concentrations to acceptable levels guided by land use. However, emerging regulatory requirements on soil protection demand a holistic view on soil assessment in remediation projects thus accounting for a variety of soil functions. Such a view would require not only that the contamination concentrations are assessed and attended to, but also that other aspects are taking into account, thus addressing also physical and biological as well as other chemical soil quality indicators (SQIs). This study outlines how soil function assessment can be a part of a holistic sustainability appraisal of remediation alternatives using multi-criteria decision analysis (MCDA). The paper presents a method for practitioners for evaluating the effects of remediation alternatives on selected ecological soil functions using a suggested minimum data set (MDS) containing physical, biological and chemical SQIs. The measured SQIs are transformed into sub-scores by the use of scoring curves, which allows interpretation and the integration of soil quality data into the MCDA framework. The method is demonstrated at a study site (Marieberg, Sweden) and the results give an example of how soil analyses using the suggested MDS can be used for soil function assessment and subsequent input to the MCDA framework.

  3. Computational Analysis of an Evolutionarily Conserved VertebrateMuscle Alternative Splicing Program

    Energy Technology Data Exchange (ETDEWEB)

    Das, Debopriya; Clark, Tyson A.; Schweitzer, Anthony; Marr,Henry; Yamamoto, Miki L.; Parra, Marilyn K.; Arribere, Josh; Minovitsky,Simon; Dubchak, Inna; Blume, John E.; Conboy, John G.

    2006-06-15

    A novel exon microarray format that probes gene expression with single exon resolution was employed to elucidate critical features of a vertebrate muscle alternative splicing program. A dataset of 56 microarray-defined, muscle-enriched exons and their flanking introns were examined computationally in order to investigate coordination of the muscle splicing program. Candidate intron regulatory motifs were required to meet several stringent criteria: significant over-representation near muscle-enriched exons, correlation with muscle expression, and phylogenetic conservation among genomes of several vertebrate orders. Three classes of regulatory motifs were identified in the proximal downstream intron, within 200nt of the target exons: UGCAUG, a specific binding site for Fox-1 related splicing factors; ACUAAC, a novel branchpoint-like element; and UG-/UGC-rich elements characteristic of binding sites for CELF splicing factors. UGCAUG was remarkably enriched, being present in nearly one-half of all cases. These studies suggest that Fox and CELF splicing factors play a major role in enforcing the muscle-specific alternative splicing program, facilitating expression of a set of unique isoforms of cytoskeletal proteins that are critical to muscle cell differentiation. Supplementary materials: There are four supplementary tables and one supplementary figure. The tables provide additional detailed information concerning the muscle-enriched datasets, and about over-represented oligonucleotide sequences in the flanking introns. The supplementary figure shows RT-PCR data confirming the muscle-enriched expression of exons predicted from the microarray analysis.

  4. An alternative transformation in ranking using l_1 -norm in data envelopment analysis

    Science.gov (United States)

    Ziari, S.

    2016-05-01

    Jahanshahloo et al. (Appl Math Comput 153:215-224, 2004) propose a method for ranking extremely efficient decision making units (DMUs) in data envelopment analysis (DEA) using super-efficiency technique and l1-norm and they show that the presented method is able to eliminate the existing difficulties in some methods. This paper suggests an alternative transformation to convert the nonlinear model proposed by Jahanshahloo et al. (Appl Math Comput 153:215-224, 2004) into a linear programming form. The present paper shows that model with this transformation is equivalent to the above-mentioned nonlinear model. The motivation of this work is to linearize the proposed nonlinear model by Jahanshahloo et al. (Appl Math Comput 153:215-224, 2004) which has the higher order of complexity.

  5. Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database

    Institute of Scientific and Technical Information of China (English)

    Bao-Long NIU; Zhi-Qi MENG; Yue-Zhi TAO; Shun-Lin LU; Hong-Biao WENG; Li-Hua HE; Wei-Feng SHEN

    2005-01-01

    We have identified Bombyx mori transformer-2 gene (Bmtra-2) cDNA by blasting the EST database of B. mori. It was expressed in the whole life of the male and female silkworm and was observed as a band of 1.3 kb by Northern blot analysis. By comparing corresponding ESTs to the Bmtra-2 DNA sequence,it was revealed that there were eight exons and seven introns, and all splice sites of exons/introns conformed to the GT/AG rule. Bmtra-2 pre-mRNA can produce multiple mRNAs encoding six distinct isoforms of BmTRA-2 protein using an alternative splicing pathway during processing. Six types of Bmtra-2 cDNA clones were identified by reverse transcription-polymerase chain reaction. All isoforms of BmTRA-2 protein contain two arginine/serine-rich domains and one RNA recognition motif, showing striking organizational similarity to Drosophila TRA-2 proteins.

  6. Genome-wide analysis of shoot growth-associated alternative splicing in moso bamboo.

    Science.gov (United States)

    Li, Long; Hu, Tao; Li, Xueping; Mu, Shaohua; Cheng, Zhanchao; Ge, Wei; Gao, Jian

    2016-08-01

    Alternative splicing (AS) significantly enhances transcriptome complexity and is differentially regulated in a wide variety of physiological processes in plants, including shoot growth. Presently, the functional implications and conservation of AS occurrences are not well understood in the moso bamboo genome. To analyze the global changes in AS during moso bamboo shoot growth, fast-growing shoots collected at seven different heights and culms after leaf expansion were sequenced using the Illumina HiSeq™ 2000 sequencing platform. It was found that approximately 60.74 % of all genes were alternatively spliced, with intron retention (IR) being the most frequent AS event (27.43 %). Statistical analysis demonstrated that variations of AS frequency and AS types were significantly correlated with changes in gene features and gene transcriptional level. According to the phylogenetic analysis of isoform expression data and AS frequency, the bamboo shoot growth could be divided into four different growth periods, including winter bamboo shoot (S1), early growth period (S2-S5), late growth period (S6 and S7), and mature period (CK). In addition, our data also showed that the winter bamboo shoot had the highest number of AS events. Twenty-six putative Serine/arginine-rich (SR) proteins were identified, producing a total of 109 transcripts. AS events were frequently and specifically regulated by SR splicing factors throughout shoot growth, resulting in changes to the original open reading frame (ORF) and subsequently changes to conserved domains. The AS product-isoforms showed regular expression change during the whole shoot growth period, thus influencing shoot growth. All together, these data indicate that AS events are adjusted to different growth stages, providing briefness and efficient means of gene regulation. This study will provide a very useful clue for future functional analyses. PMID:27170010

  7. An Alternative Dimension Reduction Approach to Supervised Principal Components Analysis in High Dimensional Survival Data

    Directory of Open Access Journals (Sweden)

    Elvan AKTURK HAYAT

    2016-04-01

    Full Text Available Objective: This study aims at comparing the performances of supervised principal component analysis (SPCA which is used for dimension reduction and an alternatively proposed approach of nonlinear principal component analysis using artificial neural networks performed by gene selection with survival tree (survival tree based NLPCA- NN. Material and Methods: Gene expression data set from Rosenwald et al.(2002 pertaining to 240 patients with diffuse large B-cell lymphoma (DLBCL is used. While Cox scores are used for determining important genes from high dimensional gene expression data in SPCA, in survival tree based NLPCA-NN approach, importance values of the survival tree are used. Important genes according to the Cox scores are reduced to three principal components by singular value decomposition. Important genes determined by the survival tree are taken as input variables in neural networks and reduced to three principal components. The performances of SPCA and survival tree based NLPCA-NN are compared by using Cox regression models (CRM. C index is calculated to compare obtained Cox regression models. Results: According to Cox scores, 121 genes are determined; according to importance values of survival tree 114 genes are determined. The percentages of variances explained by SPCA and survival tree based NLPCA-NN were 18.2% and 35.1% respectively. Harrell?s C indexes are calculated as 0.726 for CRM-1, 0.687 for CRM-2. Conclusion: As a result, while SPCA takes only the linear relationships into consideration, survival tree based NLPCA-NN also takes non-linear relationships into account and has more variance explanation and NLPCA-NN can be evaluated as an alternative method to SPCA.

  8. Genome-wide analysis of shoot growth-associated alternative splicing in moso bamboo.

    Science.gov (United States)

    Li, Long; Hu, Tao; Li, Xueping; Mu, Shaohua; Cheng, Zhanchao; Ge, Wei; Gao, Jian

    2016-08-01

    Alternative splicing (AS) significantly enhances transcriptome complexity and is differentially regulated in a wide variety of physiological processes in plants, including shoot growth. Presently, the functional implications and conservation of AS occurrences are not well understood in the moso bamboo genome. To analyze the global changes in AS during moso bamboo shoot growth, fast-growing shoots collected at seven different heights and culms after leaf expansion were sequenced using the Illumina HiSeq™ 2000 sequencing platform. It was found that approximately 60.74 % of all genes were alternatively spliced, with intron retention (IR) being the most frequent AS event (27.43 %). Statistical analysis demonstrated that variations of AS frequency and AS types were significantly correlated with changes in gene features and gene transcriptional level. According to the phylogenetic analysis of isoform expression data and AS frequency, the bamboo shoot growth could be divided into four different growth periods, including winter bamboo shoot (S1), early growth period (S2-S5), late growth period (S6 and S7), and mature period (CK). In addition, our data also showed that the winter bamboo shoot had the highest number of AS events. Twenty-six putative Serine/arginine-rich (SR) proteins were identified, producing a total of 109 transcripts. AS events were frequently and specifically regulated by SR splicing factors throughout shoot growth, resulting in changes to the original open reading frame (ORF) and subsequently changes to conserved domains. The AS product-isoforms showed regular expression change during the whole shoot growth period, thus influencing shoot growth. All together, these data indicate that AS events are adjusted to different growth stages, providing briefness and efficient means of gene regulation. This study will provide a very useful clue for future functional analyses.

  9. Regional economic analysis of current and proposed management alternatives for Rappahannock River Valley National Wildlife Refuge

    Science.gov (United States)

    Koontz, Lynne; Sexton, Natalie; Donovan, Ryan

    2009-01-01

    The National Wildlife Refuge System Improvement Act of 1997 requires all units of the National Wildlife Refuge System to be managed under a Comprehensive Conservation Plan. The Comprehensive Conservation Plan must describe the desired future conditions of a refuge and provide long-range guidance and management direction to achieve refuge purposes. The Rappahannock River Valley National Wildlife Refuge (refuge) is in the process of developing a range of management goals, objectives, and strategies for the Comprehensive Conservation Plan. The Comprehensive Conservation Plan for the refuge must contain an analysis of expected effects associated with current and proposed refuge management strategies. The purpose of this study was to assess the regional economic implications associated with draft Comprehensive Conservation Plan management strategies. Special interest groups and local residents often criticize a change in refuge management, especially if there is a perceived negative impact to the local economy. Having objective data on economic impacts may show that these fears are overstated. Quite often, the extent of economic benefits a refuge provides to a local community is not fully recognized, yet at the same time the effects of negative changes is overstated. Spending associated with refuge recreational activities, such as wildlife viewing and hunting, can generate considerable tourist activity for surrounding communities. Additionally, refuge personnel typically spend considerable amounts of money purchasing supplies in local stores, repairing equipment and purchasing fuel at the local service stations, and reside and spend their salaries in the local community. For refuge Comprehensive Conservation Plan planning, a regional economic assessment provides a means of estimating how current management (no action alternative) and proposed management activities (alternatives) could affect the local economy. This type of analysis provides two critical pieces of

  10. Analysis of Alternatives for Dismantling of the Equipment in Building 117/1 at Ignalina NPP - 13278

    Energy Technology Data Exchange (ETDEWEB)

    Poskas, Povilas; Simonis, Audrius [Lithuanian Energy Institute, Kaunas (Lithuania); Poskas, Gintautas [Lithuanian Energy Institute, Kaunas (Lithuania); Kaunas University of Technology, Kaunas (Lithuania)

    2013-07-01

    Ignalina NPP was operating two RBMK-1500 reactors which are under decommissioning now. In this paper dismantling alternatives of the equipment in Building 117/1 are analyzed. After situation analysis and collection of the primary information related to components' physical and radiological characteristics, location and other data, two different alternatives for dismantling of the equipment are formulated - the first (A1), when major components (vessels and pipes of Emergency Core Cooling System - ECCS) are segmented/halved in situ using flame cutting (oxy-acetylene) and the second one (A2), when these components are segmented/halved at the workshop using CAMC (Contact Arc Metal Cutting) technique. To select the preferable alternative MCDA method - AHP (Analytic Hierarchy Process) is applied. Hierarchical list of decision criteria, necessary for assessment of alternatives performance, are formulated. Quantitative decision criteria values for these alternatives are calculated using software DECRAD, which was developed by Lithuanian Energy Institute Nuclear engineering laboratory. While qualitative decision criteria are evaluated using expert judgment. Analysis results show that alternative A1 is better than alternative A2. (authors)

  11. Transient analysis of unbalanced short circuits of the ERDA-NASA 100 kW wind turbine alternator

    Science.gov (United States)

    Hwang, H. H.; Gilbert, L. J.

    1976-01-01

    Unbalanced short-circuit faults on the alternator of the ERDA-NASA Mod-O100-kW experimental wind turbine are studied. For each case, complete solutions for armature, field, and damper-circuit currents; short-circuit torque; and open-phase voltage are derived directly by a mathematical analysis. Formulated results are tabulated. For the Mod-O wind turbine alternator, numerical calculations are given, and results are presented by graphs. Comparisons for significant points among the more important cases are summarized. For these cases the transients are found to be potentially severe. The effect of the alternator neutral-to-ground impedance is evaluated.

  12. An analysis of news media coverage of complementary and alternative medicine.

    Directory of Open Access Journals (Sweden)

    Billie Bonevski

    Full Text Available BACKGROUND: To examine the accuracy and adequacy of lay media news stories about complementary and alternative medicines and therapies. METHODOLOGY/PRINCIPAL FINDINGS: A descriptive analysis of news stories about complementary and alternative medicine (CAM in the Australian media using a national medical news monitoring website, mediadoctor.org.au. Each story was rated against 10 criteria by two individuals. Consensus scores of 222 news articles reporting therapeutic claims about complementary medicines posted on mediadoctor.org.au between 1 January 2004 and 1 September 2007 were calculated. The overall rating score for 222 CAM articles was 50% (95% CI 47% to 53%. There was a statistically significant (F = 3.68, p = 0.006 difference in cumulative mean scores according to type of therapy: biologically based practices (54%, 95% CI 50% to 58%; manipulative body based practices (46%, 95% CI 39% to 54%, whole medical systems (45%, 95% CI 32% to 58%, mind body medicine (41%, 95% CI 31% to 50% and energy medicine (33%, 95% CI 11% to 55%. There was a statistically significant difference in cumulative mean scores (F = 3.72, p = 0.0001 according to the clinical outcome of interest with stories about cancer treatments (62%, 95% CI 54% to 70% scoring highest and stories about treatments for children's behavioural and mental health concerns scoring lowest (31%, 95% CI 19% to 43%. Significant differences were also found in scores between media outlets. CONCLUSIONS/SIGNIFICANCE: There is substantial variability in news reporting practices about CAM. Overall, although they may be improving, the scores remain generally low. It appears that much of the information the public receives about CAM is inaccurate or incomplete.

  13. Three-dimensional analysis of the Pratt and Whitney alternate design SSME fuel turbine

    Science.gov (United States)

    Kirtley, K. R.; Beach, T. A.; Adamczyk, J. J.

    1991-01-01

    The three dimensional viscous time-mean flow in the Pratt and Whitney alternate design space shuttle main engine fuel turbine is simulated using the average passage Navier-Stokes equations. The migration of secondary flows generated by upstream blade rows and their effect on the performance of downstream blade rows is studied. The present simulation confirms that the flow in this two stage turbine is highly three dimensional and dominated by the tip leakage flow. The tip leakage vortex generated by the first blade persists through the second blade and adversely affects its performance. The greatest mixing of the inlet total temperature distortion occurs in the second vane and is due to the large leakage vortex generated by the upstream rotor. It is assumed that the predominant spanwise mixing mechanism in this low aspect ratio turbine is the radial transport due to the deterministically unsteady vortical flow generated by upstream blade rows. A by-product of the analysis is accurate pressure and heat loads for all blade rows under the influence of neighboring blade rows. These aero loads are useful for advanced structural analysis of the vanes and blades.

  14. Analysis of the dynamic response improvement of a turbocharged diesel engine driven alternating current generating set

    International Nuclear Information System (INIS)

    Reliability of electric supply systems is among the most required necessities of modern society. Turbocharged diesel engine driven alternating current generating sets are often used to prevent electric black outs and/or as prime electric energy suppliers. It is well known that turbocharged diesel engines suffer from an inadequate response to a sudden load increase, this being a consequence of the nature of the energy exchange between the engine and the turbocharger. The dynamic response of turbocharged diesel engines could be improved by electric assisting systems, either by direct energy supply with an integrated starter-generator-booster (ISG) mounted on the engine flywheel, or by an indirect energy supply with an electrically assisted turbocharger. An experimentally verified zero dimensional computer simulation method was used for the analysis of both types of electrical assistance. The paper offers an analysis of the interaction between a turbocharged diesel engine and different electric assisting systems, as well as the requirements for the supporting electric motors that could improve the dynamic response of a diesel engine while driving an AC generating set. When performance class compliance is a concern, it is evident that an integrated starter-generator-booster outperforms an electrically assisted turbocharger for the investigated generating set. However, the electric energy consumption and frequency recovery times are smaller when an electrically assisted turbocharger is applied

  15. High Altitude Long Endurance UAV Analysis of Alternatives and Technology Requirements Development

    Science.gov (United States)

    Nickol, Craig L.; Guynn, Mark D.; Kohout, Lisa L.; Ozoroski, Thomas A.

    2007-01-01

    An Analysis of Alternatives and a Technology Requirements Study were conducted for two mission areas utilizing various types of High Altitude Long Endurance (HALE) Unmanned Aerial Vehicles (UAV). A hurricane science mission and a communications relay mission provided air vehicle requirements which were used to derive sixteen potential HALE UAV configurations, including heavier-than-air (HTA) and lighter-than-air (LTA) concepts with both consumable fuel and solar regenerative propulsion systems. A HTA diesel-fueled wing-body-tail configuration emerged as the preferred concept given near-term technology constraints. The cost effectiveness analysis showed that simply maximizing vehicle endurance can be a sub-optimum system solution. In addition, the HTA solar regenerative configuration was utilized to perform both a mission requirements study and a technology development study. Given near-term technology constraints, the solar regenerative powered vehicle was limited to operations during the long days and short nights at higher latitudes during the summer months. Technology improvements are required in energy storage system specific energy and solar cell efficiency, along with airframe drag and mass reductions to enable the solar regenerative vehicle to meet the full mission requirements.

  16. On the analysis of clonogenic survival data: Statistical alternatives to the linear-quadratic model

    International Nuclear Information System (INIS)

    the extraction of scores of radioresistance, which displayed significant correlations with the estimated parameters of the regression models. Undoubtedly, LQ regression is a robust method for the analysis of clonogenic survival data. Nevertheless, alternative approaches including non-linear regression and multivariate techniques such as cluster analysis and principal component analysis represent versatile tools for the extraction of parameters and/or scores of the cellular response towards ionizing irradiation with a more intuitive biological interpretation. The latter are highly informative for correlation analyses with other types of data, including functional genomics data that are increasingly beinggenerated

  17. Multi-Criteria Analysis of Alternative Energy Supply Solutions to Public Nearly Zero Energy Buildings

    OpenAIRE

    Giedrius Šiupšinskas; Solveiga Adomėnaitė

    2013-01-01

    The article analyzes energy supply alternatives for modernised public nearly zero energy buildings. The paper examines alternative energy production systems such as heat pumps (air-water and ground-water), solar collectors, adsorption cooling, biomass boiler, solar photovoltaic, wind turbines and combinations of these systems. The simulation of the analysed building energy demand for different energy production alternatives has been performed using TRNSYS modelling software. In order to deter...

  18. Alternative splicing and trans-splicing events revealed by analysis of the Bombyx mori transcriptome

    OpenAIRE

    Shao, Wei; Zhao, Qiong-Yi; Wang, Xiu-Ye; Xu, Xin-Yan; Tang, Qing; Li, Muwang; Li, Xuan; Xu, Yong-Zhen

    2012-01-01

    Alternative splicing and trans-splicing events have not been systematically studied in the silkworm Bombyx mori. Here, the silkworm transcriptome was analyzed by RNA-seq. The authors identified 320 novel genes, modified 1140 gene models, and found thousands of alternative splicing and 58 trans-splicing events. Studies of three SR proteins show that both their alternative splicing patterns and mRNA products are conserved from insect to human, and one isoform of Srsf6 with a retained intron is ...

  19. Expression microarray analysis reveals alternative splicing of LAMA3 and DST genes in head and neck squamous cell carcinoma.

    Directory of Open Access Journals (Sweden)

    Ryan Li

    Full Text Available Prior studies have demonstrated tumor-specific alternative splicing events in various solid tumor types. The role of alternative splicing in the development and progression of head and neck squamous cell carcinoma (HNSCC is unclear. Our study queried exon-level expression to implicate splice variants in HNSCC tumors.We performed a comparative genome-wide analysis of 44 HNSCC tumors and 25 uvulopalatopharyngoplasty (UPPP tissue samples at an exon expression level. In our comparison we ranked genes based upon a novel score-the Maximum-Minimum Exon Score (MMES--designed to predict the likelihood of an alternative splicing event occurring. We validated predicted alternative splicing events using quantitative RT-PCR on an independent cohort.After MMES scoring of 17,422 genes, the top 900 genes with the highest scores underwent additional manual inspection of expression patterns in a graphical analysis. The genes LAMA3, DST, VEGFC, SDHA, RASIP1, and TP63 were selected for further validation studies because of a high frequency of alternative splicing suggested in our graphical analysis, and literature review showing their biological relevance and known splicing patterns. We confirmed TP63 as having dominant expression of the short DeltaNp63 isoform in HNSCC tumor samples, consistent with prior reports. Two of the six genes (LAMA3 and DST validated by quantitative RT-PCR for tumor-specific alternative splicing events (Student's t test, P<0.001.Alternative splicing events of oncologically relevant proteins occur in HNSCC. The number of genes expressing tumor-specific splice variants needs further elucidation, as does the functional significance of selective isoform expression.

  20. ALTERNATIVES OF MACCS2 IN LANL DISPERSION ANALYSIS FOR ONSITE AND OFFSITE DOSES

    Energy Technology Data Exchange (ETDEWEB)

    Wang, John HC [Los Alamos National Laboratory

    2012-05-01

    In modeling atmospheric dispersion to determine accidental release of radiological material, one of the common statistical analysis tools used at Los Alamos National Laboratory (LANL) is MELCOR Accident Consequence Code System, Version 2 (MACCS2). MACCS2, however, has some limitations and shortfalls for both onsite and offsite applications. Alternative computer codes, which could provide more realistic calculations, are being investigated for use at LANL. In the Yucca Mountain Project (YMP), the suitability of MACCS2 for the calculation of onsite worker doses was a concern; therefore, ARCON96 was chosen to replace MACCS2. YMP's use of ARCON96 provided results which clearly demonstrated the program's merit for onsite worker safety analyses in a wide range of complex configurations and scenarios. For offsite public exposures, the conservatism of MACCS2 on the treatment of turbulence phenomena at LANL is examined in this paper. The results show a factor of at least two conservatism in calculated public doses. The new EPA air quality model, AERMOD, which implements advanced meteorological turbulence calculations, is a good candidate for LANL applications to provide more confidence in the accuracy of offsite public dose projections.

  1. Neutron activation analysis of alternative phosphate rocks used in animal nutrition

    International Nuclear Information System (INIS)

    Since 1980's, Bovine Sponghiform Encephalophaty has insidiously created a fierce battleground between farmers, scientists, environmentalists and consumers. The use of meat and bone meals is currently prohibited in ruminant feeds throughout the world. Some inorganic sources offer the combination of high phosphorus content and acceptable animal digestibility make them options as supplemental phosphorus, for instance phosphate rocks, general term applied to minerals valued chiefly for their phosphorus content. However, phosphate rocks are long been known containing hazardous elements, make them sometimes unsuitable for animal nutrition. Neutron Activation Analysis has been supportive to the mineral evaluation of alternative phosphate rocks. This evaluation is subject of on-going doctoral thesis which has been carried-out by the main author. The NAA method has been very efficient due to its highly sensitive and multi-elemental nature. In this paper results of Vanadium content from three different phosphate rocks are presented. Their values have been pointed out that Brazilian phosphate rocks present hazardous elements at the same levels of phosphate rocks from some countries of Africa, North America and Middle East, data from our study (Brazilian data) and FAO - Food and Agriculture Organization (others countries). (author)

  2. Alternative future analysis for assessing the potential impact of climate change on urban landscape dynamics.

    Science.gov (United States)

    He, Chunyang; Zhao, Yuanyuan; Huang, Qingxu; Zhang, Qiaofeng; Zhang, Da

    2015-11-01

    Assessing the impact of climate change on urban landscape dynamics (ULD) is the foundation for adapting to climate change and maintaining urban landscape sustainability. This paper demonstrates an alternative future analysis by coupling a system dynamics (SD) and a cellular automata (CA) model. The potential impact of different climate change scenarios on ULD from 2009 to 2030 was simulated and evaluated in the Beijing-Tianjin-Tangshan megalopolis cluster area (BTT-MCA). The results suggested that the integrated model, which combines the advantages of the SD and CA model, has the strengths of spatial quantification and flexibility. Meanwhile, the results showed that the influence of climate change would become more severe over time. In 2030, the potential urban area affected by climate change will be 343.60-1260.66 km(2) (5.55 -20.37 % of the total urban area, projected by the no-climate-change-effect scenario). Therefore, the effects of climate change should not be neglected when designing and managing urban landscape.

  3. Well-to-wheels life-cycle analysis of alternative fuels and vehicle technologies in China

    International Nuclear Information System (INIS)

    A well-to-wheels life cycle analysis on total energy consumptions and greenhouse-gas (GHG) emissions for alternative fuels and accompanying vehicle technologies has been carried out for the base year 2010 and projected to 2020 based on data gathered and estimates developed for China. The fuels considered include gasoline, diesel, natural gas, liquid fuels from coal conversion, methanol, bio-ethanol and biodiesel, electricity and hydrogen. Use of liquid fuels including methanol and Fischer–Tropsch derived from coal will significantly increase GHG emissions relative to use of conventional gasoline. Use of starch-based bio-ethanol will incur a substantial carbon disbenefit because of the present highly inefficient agricultural practice and plant processing in China. Electrification of vehicles via hybrid electric, plug-in hybrid electric (PHEV) and battery electric vehicle technologies offers a progressively improved prospect for the reduction of energy consumption and GHG emission. However, the long-term carbon emission reduction is assured only when the needed electricity is generated by zero- or low-carbon sources, which means that carbon capture and storage is a necessity for fossil-based feedstocks. A PHEV that runs on zero- or low-carbon electricity and cellulosic ethanol may be one of the most attractive fuel-vehicle options in a carbon-constrained world. - Highlights: ► Data and estimates unique to China are used in this analysis. ► Use of starch-based bio-ethanol will incur a substantial carbon disbenefit in China. ► Use of methanol derived from coal will incur even more carbon disbenefit. ► Plug-in-hybrid with cellulosic ethanol and clean electricity may be a viable option.

  4. Pedagogical Implications in the Thermal Analysis of Uniform Annular Fins: Alternative Analytic Solutions by Series.

    Science.gov (United States)

    Campo, Antonio; Rodriguez, Franklin

    1998-01-01

    Presents two alternative computational procedures for solving the modified Bessel equation of zero order: the Frobenius method, and the power series method coupled with a curve fit. Students in heat transfer courses can benefit from these alternative procedures; a course on ordinary differential equations is the only mathematical background that…

  5. FULL-GENOME ANALYSIS OF ALTERNATIVE SPLICING IN MOUSE LIVER AFTER HEPATOTOXICANT EXPOSURE

    Science.gov (United States)

    Alternative splicing plays a role in determining gene function and protein diversity. We have employed whole genome exon profiling using Affymetrix Mouse Exon 1.0 ST arrays to understand the significance of alternative splicing on a genome-wide scale in response to multiple toxic...

  6. ANALYSIS OF THE PROSPECTS OF SOLAR ENERGY AND OTHER ALTERNATIVE ENERGY SOURCES IN UKRAINE

    OpenAIRE

    Mogylko, O.

    2010-01-01

    The need to develop an alternative energy sources in Ukraine to increase energy efficiency and energy security it is explained in the article. The international experience of development of solar energy are analyzed. The prospects and other alternative energy sources in Ukraine are defined. The conclusions and recommendations to address the problems are identified.

  7. 78 FR 53187 - Early Scoping Notification for the Alternatives Analysis of the GA 400 Transit Initiative in...

    Science.gov (United States)

    2013-08-28

    ... capital investments. While recent legislation has led to changes in the New Starts process, MARTA will... and screen alternatives in preparation for the NEPA process. Public meetings are described immediately... three-step evaluation process includes a Fatal Flaw Analysis, Screen 1 and Screen 2 and is...

  8. Teacher Implementation of Trial-Based Functional Analysis and Differential Reinforcement of Alternative Behavior for Students with Challenging Behavior

    Science.gov (United States)

    Flynn, Susan D.; Lo, Ya-yu

    2016-01-01

    The purpose of this study was to examine the effects of a training package on three middle school special education teachers' accurate implementation of trial-based functional analysis (TBFA) and differential reinforcement of alternative behavior (DRA) with their students with autism spectrum disorders or emotional and behavioral disorders in the…

  9. Implementation of exon arrays: alternative splicing during T-cell proliferation as determined by whole genome analysis

    Directory of Open Access Journals (Sweden)

    Whistler Toni

    2010-09-01

    Full Text Available Abstract Background The contribution of alternative splicing and isoform expression to cellular response is emerging as an area of considerable interest, and the newly developed exon arrays allow for systematic study of these processes. We use this pilot study to report on the feasibility of exon array implementation looking to replace the 3' in vitro transcription expression arrays in our laboratory. One of the most widely studied models of cellular response is T-cell activation from exogenous stimulation. Microarray studies have contributed to our understanding of key pathways activated during T-cell stimulation. We use this system to examine whole genome transcription and alternate exon usage events that are regulated during lymphocyte proliferation in an attempt to evaluate the exon arrays. Results Peripheral blood mononuclear cells form healthy donors were activated using phytohemagglutinin, IL2 and ionomycin and harvested at 5 points over a 7 day period. Flow cytometry measured cell cycle events and the Affymetrix exon array platform was used to identify the gene expression and alternate exon usage changes. Gene expression changes were noted in a total of 2105 transcripts, and alternate exon usage identified in 472 transcript clusters. There was an overlap of 263 transcripts which showed both differential expression and alternate exon usage over time. Gene ontology enrichment analysis showed a broader range of biological changes in biological processes for the differentially expressed genes, which include cell cycle, cell division, cell proliferation, chromosome segregation, cell death, component organization and biogenesis and metabolic process ontologies. The alternate exon usage ontological enrichments are in metabolism and component organization and biogenesis. We focus on alternate exon usage changes in the transcripts of the spliceosome complex. The real-time PCR validation rates were 86% for transcript expression and 71% for

  10. Multi-level multi-criteria analysis of alternative fuels for waste collection vehicles in the United States.

    Science.gov (United States)

    Maimoun, Mousa; Madani, Kaveh; Reinhart, Debra

    2016-04-15

    Historically, the U.S. waste collection fleet was dominated by diesel-fueled waste collection vehicles (WCVs); the growing need for sustainable waste collection has urged decision makers to incorporate economically efficient alternative fuels, while mitigating environmental impacts. The pros and cons of alternative fuels complicate the decisions making process, calling for a comprehensive study that assesses the multiple factors involved. Multi-criteria decision analysis (MCDA) methods allow decision makers to select the best alternatives with respect to selection criteria. In this study, two MCDA methods, Technique for Order Preference by Similarity to Ideal Solution (TOPSIS) and Simple Additive Weighting (SAW), were used to rank fuel alternatives for the U.S. waste collection industry with respect to a multi-level environmental and financial decision matrix. The environmental criteria consisted of life-cycle emissions, tail-pipe emissions, water footprint (WFP), and power density, while the financial criteria comprised of vehicle cost, fuel price, fuel price stability, and fueling station availability. The overall analysis showed that conventional diesel is still the best option, followed by hydraulic-hybrid WCVs, landfill gas (LFG) sourced natural gas, fossil natural gas, and biodiesel. The elimination of the WFP and power density criteria from the environmental criteria ranked biodiesel 100 (BD100) as an environmentally better alternative compared to other fossil fuels (diesel and natural gas). This result showed that considering the WFP and power density as environmental criteria can make a difference in the decision process. The elimination of the fueling station and fuel price stability criteria from the decision matrix ranked fossil natural gas second after LFG-sourced natural gas. This scenario was found to represent the status quo of the waste collection industry. A sensitivity analysis for the status quo scenario showed the overall ranking of diesel and

  11. Molecular analysis of human argininosuccinate lyase: Mutant characterization and alternative splicing of the coding region

    International Nuclear Information System (INIS)

    Argininosuccinic acid lyase (ASAL) deficiency is a clinically heterogeneous autosomal recessive urea cycle disorder. The authors previously established by complementation analysis that 29 ASAL-deficient patients have heterogeneous mutations in a single gene. To prove that the ASAL structural gene is the affected locus, they sequenced polymerase chain reaction-amplified ASAL cDNA of a representative mutant from the single complementation group. Fibroblast strain 944 from a late-onset patient who was the product of a consanguineous mating, had only a single base-pair change in the coding region, a C-283→ T transition at a CpG dinucleotide in exon 3. This substitution converts Arg-95 to Cys (R95C), occurs in a stretch of 13 residues that is identical in yeast and human ASAL, and was present in both of the patient's alleles but not in 14 other mutant or 10 normal alleles. They observed that amplified cDNA from mutant 944 and normal cells (liver, keratinocytes, lymphoblasts, and fibroblasts) contained, in addition to the expected 5' 513-base-pair band, a prominent 318-base-pair ASAL band formed by the splicing of exon 2 from the transcript. The short transcript maintains the ASAL reading frame but removes Lys-51, a residue that may be essential for catalysis, since it binds the argininosuccinate substrate. They conclude (i) that the identification of the R95C mutation in strain 944 demonstrates that virtually all ASAL deficiency results from defects in the ASAL structural gene and (ii) that minor alternative splicing of the coding region occurs at the ASAL locus

  12. Remote-Handled Low-Level Waste Disposal Project Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    David Duncan

    2011-04-01

    This report identifies, evaluates, and compares alternatives for meeting the U.S. Department of Energy’s mission need for management of remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Each alternative identified in the Mission Need Statement for the Remote-Handled Low-Level Waste Treatment Project is described and evaluated for capability to fulfill the mission need. Alternatives that could meet the mission need are further evaluated and compared using criteria of cost, risk, complexity, stakeholder values, and regulatory compliance. The alternative for disposal of remote-handled low-level waste that has the highest confidence of meeting the mission need and represents best value to the government is to build a new disposal facility at the Idaho National Laboratory Site.

  13. Remote-Handled Low-Level Waste Disposal Project Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    David Duncan

    2011-03-01

    This report identifies, evaluates, and compares alternatives for meeting the U.S. Department of Energy’s mission need for management of remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Each alternative identified in the Mission Need Statement for the Remote-Handled Low-Level Waste Treatment Project is described and evaluated for capability to fulfill the mission need. Alternatives that could meet the mission need are further evaluated and compared using criteria of cost, risk, complexity, stakeholder values, and regulatory compliance. The alternative for disposal of remote-handled low-level waste that has the highest confidence of meeting the mission need and represents best value to the government is to build a new disposal facility at the Idaho National Laboratory Site.

  14. Remote-Handled Low-Level Waste Disposal Project Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    David Duncan

    2009-10-01

    This report identifies, evaluates, and compares alternatives for meeting the U.S. Department of Energy’s mission need for management of remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Each alternative identified in the Mission Need Statement for the Remote-Handled Low-Level Waste Treatment Project is described and evaluated for capability to fulfill the mission need. Alternatives that could meet the mission need are further evaluated and compared using criteria of cost, risk, complexity, stakeholder values, and regulatory compliance. The alternative for disposal of remote-handled low-level waste that has the highest confidence of meeting the mission need and represents best value to the government is to build a new disposal facility at the Idaho National Laboratory Site.

  15. Portfolio theory and the alternative decision rule of cost-effectiveness analysis: theoretical and practical considerations.

    Science.gov (United States)

    Sendi, Pedram; Al, Maiwenn J; Gafni, Amiram; Birch, Stephen

    2004-05-01

    Bridges and Terris (Soc. Sci. Med. (2004)) critique our paper on the alternative decision rule of economic evaluation in the presence of uncertainty and constrained resources within the context of a portfolio of health care programs (Sendi et al. Soc. Sci. Med. 57 (2003) 2207). They argue that by not adopting a formal portfolio theory approach we overlook the optimal solution. We show that these arguments stem from a fundamental misunderstanding of the alternative decision rule of economic evaluation. In particular, the portfolio theory approach advocated by Bridges and Terris is based on the same theoretical assumptions that the alternative decision rule set out to relax. Moreover, Bridges and Terris acknowledge that the proposed portfolio theory approach may not identify the optimal solution to resource allocation problems. Hence, it provides neither theoretical nor practical improvements to the proposed alternative decision rule.

  16. Remote-Handled Low-Level Waste Disposal Project Alternatives Analysis

    Energy Technology Data Exchange (ETDEWEB)

    David Duncan

    2010-06-01

    This report identifies, evaluates, and compares alternatives for meeting the U.S. Department of Energy’s mission need for management of remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Each alternative identified in the Mission Need Statement for the Remote-Handled Low-Level Waste Treatment Project is described and evaluated for capability to fulfill the mission need. Alternatives that could meet the mission need are further evaluated and compared using criteria of cost, risk, complexity, stakeholder values, and regulatory compliance. The alternative for disposal of remote-handled low-level waste that has the highest confidence of meeting the mission need and represents best value to the government is to build a new disposal facility at the Idaho National Laboratory Site.

  17. Design of batch operations: Systematic methodology for generation and analysis of sustainable alternatives

    DEFF Research Database (Denmark)

    Carvalho, Ana; Matos, Henrique A.; Gani, Rafiqul

    2010-01-01

    The objective of this paper is to present a new methodology that is able to generate, screen and identify sustainable alternatives to continuous chemical processes as well as processes operating in the batch mode. The methodology generates the sustainable (design) alternatives by locating...... processes are described, highlighting the main differences between them. Through two case studies, the application of the methodology, to obtain sustainable design alternatives for batch plants, is highlighted....... the operational, environmental, economical and safety related problems inherent in the process (batch or continuous). Alternatives that are more sustainable, compared to a reference, are generated and evaluated by addressing one or more of the identified problems. A decomposition technique as well as a set...

  18. Transcriptome Bioinformatical Analysis of Vertebrate Stages of Schistosoma japonicum Reveals Alternative Splicing Events

    OpenAIRE

    Xinye Wang; Xindong Xu; Xingyu Lu; Yuanbin Zhang; Weiqing Pan

    2015-01-01

    Alternative splicing is a molecular process that contributes greatly to the diversification of proteome and to gene functions. Understanding the mechanisms of stage-specific alternative splicing can provide a better understanding of the development of eukaryotes and the functions of different genes. Schistosoma japonicum is an infectious blood-dwelling trematode with a complex lifecycle that causes the tropical disease schistosomiasis. In this study, we analyzed the transcriptome of Schistoso...

  19. Electrical signature analysis applications for non-intrusive automotive alternator diagnostics

    Energy Technology Data Exchange (ETDEWEB)

    Ayers, C.W.

    1996-03-01

    Automotive alternators are designed to supply power for automobile engine ignition systems as well as charge the storage battery. This product is used in a large market where consumers are concerned with acoustic noise and vibration that comes from the unit. as well as overall quality and dependability. Alternators and generators in general are used in industries other than automotive, such as transportation and airline industries and in military applications. Their manufacturers are interested in pursuing state-of-the-art methods to achieve higher quality and reduced costs. Preliminary investigations of non-intrusive diagnostic techniques utilizing the inherent voltage signals of alternators have been performed with promising results. These techniques are based on time and frequency domain analyses of specially conditioned signals taken from several alternators under various test conditions. This paper discusses investigations that show correlations of the alternator output voltage to airborne noise production. In addition these signals provide insight into internal magnetic characteristics that relate to design and/or assembly problems.

  20. The analysis of covariance and alternatives statistical methods for experiments, quasi-experiments, and single-case studies

    CERN Document Server

    Huitema, Bradley

    2011-01-01

    A complete guide to cutting-edge techniques and best practices for applying covariance analysis methods The Second Edition of Analysis of Covariance and Alternatives sheds new light on its topic, offering in-depth discussions of underlying assumptions, comprehensive interpretations of results, and comparisons of distinct approaches. The book has been extensively revised and updated to feature an in-depth review of prerequisites and the latest developments in the field. The author begins with a discussion of essential topics relating to experimental design and analysis

  1. How robust is a robust policy? A comparative analysis of alternative robustness metrics for supporting robust decision analysis.

    Science.gov (United States)

    Kwakkel, Jan; Haasnoot, Marjolijn

    2015-04-01

    In response to climate and socio-economic change, in various policy domains there is increasingly a call for robust plans or policies. That is, plans or policies that performs well in a very large range of plausible futures. In the literature, a wide range of alternative robustness metrics can be found. The relative merit of these alternative conceptualizations of robustness has, however, received less attention. Evidently, different robustness metrics can result in different plans or policies being adopted. This paper investigates the consequences of several robustness metrics on decision making, illustrated here by the design of a flood risk management plan. A fictitious case, inspired by a river reach in the Netherlands is used. The performance of this system in terms of casualties, damages, and costs for flood and damage mitigation actions is explored using a time horizon of 100 years, and accounting for uncertainties pertaining to climate change and land use change. A set of candidate policy options is specified up front. This set of options includes dike raising, dike strengthening, creating more space for the river, and flood proof building and evacuation options. The overarching aim is to design an effective flood risk mitigation strategy that is designed from the outset to be adapted over time in response to how the future actually unfolds. To this end, the plan will be based on the dynamic adaptive policy pathway approach (Haasnoot, Kwakkel et al. 2013) being used in the Dutch Delta Program. The policy problem is formulated as a multi-objective robust optimization problem (Kwakkel, Haasnoot et al. 2014). We solve the multi-objective robust optimization problem using several alternative robustness metrics, including both satisficing robustness metrics and regret based robustness metrics. Satisficing robustness metrics focus on the performance of candidate plans across a large ensemble of plausible futures. Regret based robustness metrics compare the

  2. Alternative splicing in colon, bladder, and prostate cancer identified by exon-array analysis

    DEFF Research Database (Denmark)

    Thorsen, Kasper; Sørensen, Karina D.; Brems-Eskildsen, Anne Sofie;

    2008-01-01

    Alternative splicing enhances proteome diversity and modulates cancer-associated proteins. To identify tissue- and tumor-specific alternative splicing, we used the GeneChip Human Exon 1.0 ST Array to measure whole-genome exon expression in 102 normal and cancer tissue samples of different stages......, and 18 candidate tumor-specific splicing alterations in colon, bladder, and prostate, respectively, were selected for RT-PCR validation on an independent set of 81 normal and tumor tissue samples. In total, seven genes with tumor-specific splice variants were identified (ACTN1, CALD1, COL6A3, LRRFIP2...... from colon, urinary bladder, and prostate. We identified 2069 candidate alternative splicing events between normal tissue samples from colon, bladder, and prostate and selected 15 splicing events for RT-PCR validation, 10 of which were successfully validated by RT-PCR and sequencing. Furthermore 23, 19...

  3. Alternatives Analysis Amchitka Island Mud Pit Cap Repair, Amchitka, Alaska January 2016

    Energy Technology Data Exchange (ETDEWEB)

    Darr, Paul S. [US Department of Energy, Washington, DC (United States). Office of Legacy Management

    2016-01-01

    The U.S. Department of Energy (DOE) Office of Legacy Management (LM) manages the Nevada Offsites program, which includes a series of reclaimed drilling mud impoundments on Amchitka Island, Alaska (Figure 1). Navarro Research and Engineering, Inc. is the Legacy Management Support contractor (the Contractor) for LM. The Contractor has procured Tetra Tech, Inc. to provide engineering support to the Amchitka mud pit reclamation project. The mud pit caps were damaged during a 7.9-magnitude earthquake that occurred in 2014. The goals of the current project are to investigate conditions at the mud pit impoundments, identify feasible alternatives for repair of the cover systems and the contents, and estimate relative costs of repair alternatives. This report presents descriptions of the sites and past investigations, existing conditions, summaries of various repair/mitigation alternatives, and direct, unburdened, order-of-magnitude (-15% to +50%) associated costs.

  4. Analysis of different alternatives for a reactor aimed to produce Co60. Neutronic part

    International Nuclear Information System (INIS)

    Different alternatives are presented for a reactor aimed to produce radioisotopes with a low enrichment fuel element geometrically equal to those of low enrichment. Different alternatives are presented which essentially differ in the core's configuration, fuel management and 'meat' fuel material: U3O8, U3Si or U3Si2 in aluminium matrix. Fluxes at different irradiation positions and power full peak factors are described for each case. The main aspects to be taken into account for cobalt irradiation and the method to be followed to measure the reactor as a function of a required production are presented. (Author)

  5. 75 FR 30100 - FY 2010 Discretionary Livability Funding Opportunity: Alternatives Analysis Program

    Science.gov (United States)

    2010-05-28

    ... planning organizations, and local governmental authorities to conduct alternatives analyses as defined by... Work Program of the metropolitan planning organization for the area. Applicants must commit to begin... of Planning and Environment, by phone at (202) 493-0512 or by e-mail at Kenneth.Cervenka@dot.gov ....

  6. Studying Effects of Transcranial Alternating Current Stimulation on Hearing and Auditory Scene Analysis

    NARCIS (Netherlands)

    Riecke, Lars

    2016-01-01

    Recent studies have shown that perceptual detection of near-threshold auditory events may depend on the relative timing of the event and ongoing brain oscillations. Furthermore, transcranial alternating current stimulation (tACS), a non-invasive and silent brain stimulation technique, can entrain co

  7. An Empirical Analysis of an Alternative Configuration of the Academic Motivation Scale

    Science.gov (United States)

    Smith, Kenneth J.; Davy, Jeanette A.; Rosenberg, Donald L.

    2012-01-01

    The purpose of this study was to examine an alternative configuration of the Academic Motivation Scale (AMS), a psychometric instrument used as a measure of academic motivation in various academic environments. The analyses utilised data from a convenience sample of 2354 business students, broken into two random subsamples of 1177 cases.…

  8. Alternative security

    International Nuclear Information System (INIS)

    This book contains the following chapters: The Military and Alternative Security: New Missions for Stable Conventional Security; Technology and Alternative Security: A Cherished Myth Expires; Law and Alternative Security: Toward a Just World Peace; Politics and Alternative Security: Toward a More Democratic, Therefore More Peaceful, World; Economics and Alternative Security: Toward a Peacekeeping International Economy; Psychology and Alternative Security: Needs, Perceptions, and Misperceptions; Religion and Alternative Security: A Prophetic Vision; and Toward Post-Nuclear Global Security: An Overview

  9. Benefits and risks of emerging technologies: integrating life cycle assessment and decision analysis to assess lumber treatment alternatives.

    Science.gov (United States)

    Tsang, Michael P; Bates, Matthew E; Madison, Marcus; Linkov, Igor

    2014-10-01

    Assessing the best options among emerging technologies (e.g., new chemicals, nanotechnologies) is complicated because of trade-offs across benefits and risks that are difficult to quantify given limited and fragmented availability of information. This study demonstrates the integration of multicriteria decision analysis (MCDA) and life cycle assessment (LCA) to address technology alternative selection decisions. As a case study, prioritization of six lumber treatment alternatives [micronized copper quaternary (MCQ); alkaline copper quaternary (ACQ); water-borne copper naphthenate (CN); oil-borne copper naphthenate (CNo); water-borne copper quinolate (CQ); and water-borne zinc naphthenate (ZN)] for military use are considered. Multiattribute value theory (MAVT) is used to derive risk and benefit scores. Risk scores are calculated using a cradle-to-gate LCA. Benefit scores are calculated by scoring of cost, durability, and corrosiveness criteria. Three weighting schemes are used, representing Environmental, Military and Balanced stakeholder perspectives. Aggregated scores from all three perspectives show CQ to be the least favorable alterative. MCQ is identified as the most favorable alternative from the Environmental stakeholder perspective. From the Military stakeholder perspective, ZN is determined to be the most favorable alternative, followed closely by MCQ. This type of scoring and ranking of multiple heterogeneous criteria in a systematic and transparent way facilitates better justification of technology selection and regulation. PMID:25209330

  10. Low-Level Waste Disposal Alternatives Analysis for Low-Level Waste Generated at the Idaho National Laboratory

    International Nuclear Information System (INIS)

    This paper presents the results of a study that identifies and compares on-site and off-site disposal options for the disposal of contact-handled and remote-handled low-level waste generated by the Idaho National Laboratory and its tenants. Potential disposal options are screened for viability by waste type resulting in a short list of options for further consideration. The most credible options are selected after a systematic consideration of cost, schedule constraints, and risk. In order to holistically address the approach for low-level waste disposal, options are compiled into comprehensive disposal schemes, that is, alternative scenarios. Each alternative scenario addresses the disposal path for all low-level waste types over the period of interest. The alternative scenarios are compared and ranked using cost, risk and complexity to arrive at the recommended approach. Schedule alignment with disposal needs is addressed to ensure that all waste types are managed appropriately. The recommended alternative scenario for the disposal of low-level waste based on this analysis is to build a disposal facility at the Idaho National Laboratory Site. (authors)

  11. 2-Methoxyethylamine as Co2 absorber alternative for analysis of 14C content in soil and groundwater samples

    International Nuclear Information System (INIS)

    At the hydrology laboratory PATIR-BATAN, 14C analysis using Carbosorb as an absorber of CO2 has been developed and applied to several research activities especially researches related to soil and groundwater. However, at the present time, Packard Co. as a producer of carbosorb is no longer producing carbosorb hence it is hardly found in the market. Therefore, the alternatives of CO2 absorber should be found. 2-Methoxyethylamine is considered to be a good alternative and it is commercially available. The study is intended to elaborate the potential of 2-methoxyethylamine as an alternative absorber to analyze 14C content. Several times of analysis showed that the solution of 2 methoxyethylamine/scintillator at 21 ml is able to absorb 2.61-3.08 g CO2 or equivalent to 0.713 -0.810 g of 14C at saturated temperature of 53oC. At this time, the density of the solutions increased from 0.866 g/ml to around 0.974 g/ml. By three times test, the results of background and standard counting were relatively stable with values 20.36 ± 0.10 cpm and 32.74 ± 0.06 cpm, respectively. Whereas, the results of several samples analysis showed that all the 14C count of soil and groundwater during this study were in between the count of background and standard. This result shows that 2 methoxyethylamine can serve as an alternative of CO2 absorber to determine the age of soil and groundwater samples through their 14C contents. (author)

  12. A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells.

    Science.gov (United States)

    Shang, Jin; Wang, Honggang; Fan, Xin; Shangguan, Lei; Liu, Huan

    2016-08-01

    Low back pain is a prevalent disease, which leads to suffering and disabilities in a vast number of individuals. Degenerative disc diseases are usually the underlying causes of low back pain. However, the pathogenesis of degenerative disc diseases is highly complex and difficult to determine. Current therapies for degenerative disc diseases are various. In particular, cell-based therapies have proven to be effective and promising. Our research group has previously isolated and identified the cartilage endplate‑derived stem cells. In addition, alternative splicing is a sophisticated regulatory mechanism, which greatly increases cellular complexity and phenotypic diversity of eukaryotic organisms. The present study continued to investigate alternative splicing events in osteogenic differentiation of cartilage endplate‑derived stem cells. An Affymetrix Human Transcriptome Array 2.0 was used to detect splicing changes between the control and differentiated samples. Additionally, molecular function and pathway analysis were also performed. Following rigorous bioinformatics analysis of the data, 3,802 alternatively spliced genes were identified, and 10 of these were selected for validation by reverse transcription‑polymerase chain reaction. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway analysis also revealed numerous enriched GO terms and signaling pathways. To the best of our knowledge, the present study is the first to investigate alternative splicing mechanisms in osteogenic differentiation of stem cells on a genome‑wide scale. The illumination of molecular mechanisms of stem cell osteogenic differentiation may assist the development novel bioengineered methods to treat degenerative disc diseases.

  13. A genome wide analysis of alternative splicing events during the osteogenic differentiation of human cartilage endplate-derived stem cells.

    Science.gov (United States)

    Shang, Jin; Wang, Honggang; Fan, Xin; Shangguan, Lei; Liu, Huan

    2016-08-01

    Low back pain is a prevalent disease, which leads to suffering and disabilities in a vast number of individuals. Degenerative disc diseases are usually the underlying causes of low back pain. However, the pathogenesis of degenerative disc diseases is highly complex and difficult to determine. Current therapies for degenerative disc diseases are various. In particular, cell-based therapies have proven to be effective and promising. Our research group has previously isolated and identified the cartilage endplate‑derived stem cells. In addition, alternative splicing is a sophisticated regulatory mechanism, which greatly increases cellular complexity and phenotypic diversity of eukaryotic organisms. The present study continued to investigate alternative splicing events in osteogenic differentiation of cartilage endplate‑derived stem cells. An Affymetrix Human Transcriptome Array 2.0 was used to detect splicing changes between the control and differentiated samples. Additionally, molecular function and pathway analysis were also performed. Following rigorous bioinformatics analysis of the data, 3,802 alternatively spliced genes were identified, and 10 of these were selected for validation by reverse transcription‑polymerase chain reaction. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes pathway analysis also revealed numerous enriched GO terms and signaling pathways. To the best of our knowledge, the present study is the first to investigate alternative splicing mechanisms in osteogenic differentiation of stem cells on a genome‑wide scale. The illumination of molecular mechanisms of stem cell osteogenic differentiation may assist the development novel bioengineered methods to treat degenerative disc diseases. PMID:27278552

  14. Thermodynamic Analysis of Alternative Marine Fuels for Marine Gas Turbine Power Plants

    Institute of Scientific and Technical Information of China (English)

    Mohamed M El Gohary; Nader R Ammar

    2016-01-01

    The marine shipping industry faces challenges to reduce engine exhaust emissions and greenhouse gases (GHGs) from ships, and in particular, carbon dioxide. International regulatory bodies such as the International Maritime Organization and National Environmental Agencies of many countries have issued rules and regulations to drastically reduce GHG and emissions emanating from marine sources. This study investigates the possibility of using natural gas and hydrogen as alternative fuels to diesel oil for marine gas turbines and uses a mathematical model to assess the effect of these alternative fuels on gas turbine thermodynamic performance. Results show that since natural gas is categorized as a hydrocarbon fuel, the thermodynamic performance of the gas turbine cycle using natural gas was close to that of the diesel case. However, the gas turbine thermal efficiency was found to be slightly lower for natural gas and hydrogen fuels compared to diesel fuel.

  15. Thermodynamic analysis of alternative marine fuels for marine gas turbine power plants

    Science.gov (United States)

    El Gohary, Mohamed M.; Ammar, Nader R.

    2016-03-01

    The marine shipping industry faces challenges to reduce engine exhaust emissions and greenhouse gases (GHGs) from ships, and in particular, carbon dioxide. International regulatory bodies such as the International Maritime Organization and National Environmental Agencies of many countries have issued rules and regulations to drastically reduce GHG and emissions emanating from marine sources. This study investigates the possibility of using natural gas and hydrogen as alternative fuels to diesel oil for marine gas turbines and uses a mathematical model to assess the effect of these alternative fuels on gas turbine thermodynamic performance. Results show that since natural gas is categorized as a hydrocarbon fuel, the thermodynamic performance of the gas turbine cycle using natural gas was close to that of the diesel case. However, the gas turbine thermal efficiency was found to be slightly lower for natural gas and hydrogen fuels compared to diesel fuel.

  16. Three-D Flow Analysis of the Alternate SSME HPOT TAD

    Science.gov (United States)

    Kubinski, Cheryl A.

    1993-01-01

    This paper describes the results of numerical flow analyses performed in support of design development of the Space Shuttle Main Engine Alternate High Pressure Oxidizer Turbine Turn-around duct (TAD). The flow domain has been modeled using a 3D, Navier-Stokes, general purpose flow solver. The goal of this effort is to achieve an alternate TAD exit flow distribution which closely matches that of the baseline configuration. 3D Navier Stokes CFD analyses were employed to evaluate numerous candidate geometry modifications to the TAD flowpath in order to achieve this goal. The design iterations are summarized, as well as a description of the computational model, numerical results and the conclusions based on these calculations.

  17. Alternatives generation and analysis for double-shell tank primary ventilation systems emissions control and monitoring

    Energy Technology Data Exchange (ETDEWEB)

    SEDERBURG, J.P.

    1999-09-30

    This AGA addresses the question: ''What equipment upgrades, operational changes, and/or other actions are required relative to the DST tanks farms' ventilation systems to support retrieval, staging (including feed sampling), and delivery of tank waste to the Phase I private contractor?'' Issues and options for the various components within the ventilation subsystem affect each other. Recommended design requirements are presented and the preferred alternatives are detailed.

  18. Socio-economic impact analysis: Centralia mine fire abatement alternatives. Draft report

    Energy Technology Data Exchange (ETDEWEB)

    1980-11-07

    The overall purpose of information contained in the following text is to document the likely social and economic impacts upon the Borough of Centralia through implementation of various mine fire abatement alternatives. Much of the data presented herein and utilized in preparing conclusions and recommendations have been derived from those individuals whose lives are now, or may eventually be, impacted by the underground mine fire.

  19. Analysis of the relevance of crowdfunding as an alternative source of finance for social enterprises

    OpenAIRE

    Priede Bergamini, Tiziana; López-Cózar Navarro, Cristina; Hilliard, Ivan

    2015-01-01

    Social entrepreneurship is the creation of a business project based on the identification and resolution of a social or environmental issue. Although recent years have seen an increase in published articles addressing various aspects of social entrepreneurship, the number of works dealing specifically with funding is quite limited, and even more so in the case of those which focus on alternative financing options such as crowdfunding. Therefore, the main objective of this paper is to analyze ...

  20. Immobilized high-level waste interim storage alternatives generation and analysis and decision report

    Energy Technology Data Exchange (ETDEWEB)

    CALMUS, R.B.

    1999-05-18

    This report presents a study of alternative system architectures to provide onsite interim storage for the immobilized high-level waste produced by the Tank Waste Remediation System (TWRS) privatization vendor. It examines the contract and program changes that have occurred and evaluates their impacts on the baseline immobilized high-level waste (IHLW) interim storage strategy. In addition, this report documents the recommended initial interim storage architecture and implementation path forward.

  1. Analysis of Alternative Institutional Arrangements for Reform of U.S. Air Traffic Control

    OpenAIRE

    Lewis, Ira

    2004-01-01

    A considerable amount of New Public Management-oriented research investigates alternative institutional arrangements for provision of services to the public. Some of this work argues in support of service delivery through an increase in outsourcing or by privatization of existing government functions. Air traffic control is provided to aircraft operators using airports and airspace all over the world. This article studies institutional arrangements of provision of air traffic c...

  2. Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens

    OpenAIRE

    Wu, Hshin-Ping; Su, Yi-shin; Chen, Hsiu-Chen; Chen, Yu-Rong; Wu, Chia-Chen; Lin, Wen-Dar; Tu, Shih-Long

    2014-01-01

    Background Light is one of the most important factors regulating plant growth and development. Light-sensing photoreceptors tightly regulate gene expression to control photomorphogenic responses. Although many levels of gene expression are modulated by photoreceptors, regulation at the mRNA splicing step remains unclear. Results We performed high-throughput mRNA sequencing to analyze light-responsive changes in alternative splicing in the moss Physcomitrella patens, and found that a large num...

  3. Phrasal alternation in the Pondok Tinggi dialect of Kerinci; An intergenerational analysis

    Directory of Open Access Journals (Sweden)

    E. Ernanda

    2015-07-01

    Full Text Available This paper examines the implications of language contact in a Malay sub-variety known as Pondok Tinggi, spoken in Sumatra. My focus is on the grammatical phenomenon of phrasal alternation. Phrasal alternation is characterized by the presence of two distinct forms for nearly all lexical items, whose final syllables differ in shape. These are termed absolute and oblique (Steinhauer and Usman 1978: 485. The intergenerational transmission of this uncommon feature offers a way to measure the degree of contact-induced language change in Pondok Tinggi. An experiment was conducted to elicit the usage of the absolute and the oblique forms in order to find out how the distribution of phrasal alternation has changed within the last two generations. I reveal a grammatical simplification caused by contact between Pondok Tinggi and Bahasa Indonesia, a related Malayic variety serving as Indonesia’s prestigious official language. This adds a dimension of loss of local linguistic diversity to more familiar tropes of the national success of Bahasa Indonesia.

  4. Alternative energies; Energies alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Bonal, J.; Rossetti, P

    2007-07-01

    The earth took millions years to made the petroleum, the gas the coal and the uranium. Only a few centuries will be needed to exhaust these fossil fuels and some years to reach expensive prices. Will the wold continue on this way of energy compulsive consumption? The renewable energies and some citizen attitudes are sufficient to break this spiral. This book proposes to discuss these alternative energies. It shows that this attitude must be supported by the government. It takes stock on the more recent information concerning the renewable energies. it develops three main points: the electricity storage, the housing and the transports. (A.L.B.)

  5. Genome-wide analysis of alternative splicing of pre-mRNA under salt stress in Arabidopsis

    KAUST Repository

    Ding, Feng

    2014-06-04

    Background: Alternative splicing (AS) of precursor mRNA (pre-mRNA) is an important gene regulation process that potentially regulates many physiological processes in plants, including the response to abiotic stresses such as salt stress.Results: To analyze global changes in AS under salt stress, we obtained high-coverage (~200 times) RNA sequencing data from Arabidopsis thaliana seedlings that were treated with different concentrations of NaCl. We detected that ~49% of all intron-containing genes were alternatively spliced under salt stress, 10% of which experienced significant differential alternative splicing (DAS). Furthermore, AS increased significantly under salt stress compared with under unstressed conditions. We demonstrated that most DAS genes were not differentially regulated by salt stress, suggesting that AS may represent an independent layer of gene regulation in response to stress. Our analysis of functional categories suggested that DAS genes were associated with specific functional pathways, such as the pathways for the responses to stresses and RNA splicing. We revealed that serine/arginine-rich (SR) splicing factors were frequently and specifically regulated in AS under salt stresses, suggesting a complex loop in AS regulation for stress adaptation. We also showed that alternative splicing site selection (SS) occurred most frequently at 4 nucleotides upstream or downstream of the dominant sites and that exon skipping tended to link with alternative SS.Conclusions: Our study provided a comprehensive view of AS under salt stress and revealed novel insights into the potential roles of AS in plant response to salt stress. 2014 Ding et al.; licensee BioMed Central Ltd.

  6. Technology and alternative cancer therapies: an analysis of heterodoxy and constructivism.

    Science.gov (United States)

    Hess, D J

    1996-12-01

    Theories of the construction of technology are reviewed from the wider interdisciplinary conversation known as science and technology studies (STS) and from the growing field of the anthropology of science and technology. These theories are used to contribute to research situated at the intersection of the anthropology of alternative medicine and of medical technologies. Cases drawn from the research tradition on microbial theories of cancer are considered to show how unorthodox medical theories become embedded in technologies through choices in microscope design and treatment technologies. In turn, the technologies contribute to the heterodox standing of the researchers, their research, and their therapies.

  7. Phylogenetic Analysis of Gene Structure and Alternative Splicing in α-Actinins

    OpenAIRE

    Lek, Monkol; MacArthur, Daniel G.; Yang, Nan; North, Kathryn N.

    2009-01-01

    The α-actinins are an important family of actin-binding proteins with the ability to cross-link actin filaments when in dimer form. Members of the α-actinin family share a domain topology composed of highly conserved actin-binding and EF-hand domains separated by a rod domain composed of spectrin-like repeats. Functional diversity within this family has arisen through exon duplication and the formation of alternate splice isoforms as well as gene duplications during the evolution of vertebrat...

  8. Alternative additives; Alternative additiver

    Energy Technology Data Exchange (ETDEWEB)

    2007-08-15

    In this project a number of industrial and agricultural waste products have been characterised and evaluated in terms of alkali-getter performance. The intended use is for biomass-fired power stations aiming at reducing corrosion or slagging related problems. The following products have been obtained, characterised and evaluated: 1) Brewery draff 2) Danish de-gassed manure 3) Paper sludge 4) Moulding sand 5) Spent bleaching earth 6) Anorthosite 7) Sand 8) Clay-sludge. Most of the above alternative additive candidates are deemed unsuitable due to insufficient chemical effect and/or expensive requirements for pre-treatment (such as drying and transportation). 3 products were selected for full-scale testing: de-gassed manure, spent bleaching earth and clay slugde. The full scale tests were undertaken at the biomass-fired power stations in Koege, Slagelse and Ensted. Spent bleaching earth (SBE) and clay sludge were the only tested additive candidates that had a proven ability to react with KCl, to thereby reduce Cl-concentrations in deposits, and reduce the deposit flux to superheater tubes. Their performance was shown to nearly as good as commercial additives. De-gassed manure, however, did not evaluate positively due to inhibiting effects of Ca in the manure. Furthermore, de-gassed manure has a high concentration of heavy metals, which imposes a financial burden with regard to proper disposal of the ash by-products. Clay-sludge is a wet clay slurring, and drying and transportation of this product entails substantial costs. Spent bleaching does not require much pre-treatment and is therefore the most promising alternative additive. On the other hand, bleaching earth contains residual plant oil which means that a range of legislation relating to waste combustion comes into play. Not least a waste combustion fee of 330 DKK/tonne. For all alternative (and commercial) additives disposal costs of the increase ash by-products represents a significant cost. This is

  9. Computational analysis of translational readthrough proteins in Drosophila and yeast reveals parallels to alternative splicing.

    Science.gov (United States)

    Pancsa, Rita; Macossay-Castillo, Mauricio; Kosol, Simone; Tompa, Peter

    2016-01-01

    In translational readthrough (TR) the ribosome continues extending the nascent protein beyond the first in-frame termination codon. Due to the lack of dedicated analyses of eukaryotic TR cases, the associated functional-evolutionary advantages are still unclear. Here, based on a variety of computational methods, we describe the structural and functional properties of previously proposed D. melanogaster and S. cerevisiae TR proteins and extensions. We found that in D. melanogaster TR affects long proteins in mainly regulatory roles. Their TR-extensions are structurally disordered and rich in binding motifs, which, together with their cell-type- and developmental stage-dependent inclusion, suggest that similarly to alternatively spliced exons they rewire cellular interaction networks in a temporally and spatially controlled manner. In contrast, yeast TR proteins are rather short and fulfil mainly housekeeping functions, like translation. Yeast extensions usually lack disorder and linear motifs, which precludes elucidating their functional relevance with sufficient confidence. Therefore we propose that by being much more restricted and by lacking clear functional hallmarks in yeast as opposed to fruit fly, TR shows remarkable parallels with alternative splicing. Additionally, the lack of conservation of TR extensions among orthologous TR proteins suggests that TR-mediated functions may be generally specific to lower taxonomic levels. PMID:27561673

  10. Analysis of words to development of augmentative and alternative communication boards for disabled student

    Directory of Open Access Journals (Sweden)

    Andréa Carla Paura

    2011-12-01

    Full Text Available Purpose: The aim of this study was to analyze the contribution of the words used in language assessment instruments and/or the vocabulary used in Brazil for the development of alternative communication boards. Methods: word lists from the selected assessment instruments were analyzed through a protocol designed for this purpose. The frequency of occurrence of each word was verified considering four word lists from the instruments and the frequency of occurrence of these words according to the classification proposed by Comunication Pictu Symbols system - PCS. Results: Results showed that the frequency words occurred only once was of 67.88% and the frequency of occurrence of concrete and abstract nouns in the instruments was 60.04%. The instrument that presented words with more than one occurrence was the Vocabulary Test-PPVT Peabody Picture. Conclusions: The use of tool that are already used and standardized may contribute to the process of evaluation, selection and deployment of augmentative and alternative communication resources for children and youth with disabilities.

  11. Input-output analysis of alternative policies implemented on the energy activities: An application for Catalonia

    International Nuclear Information System (INIS)

    The aim of this paper is to analyze the economic impact of alternative policies implemented on the energy activities of the Catalan production system. Specifically, we analyze the effects of a tax on intermediate energy uses, a reduction in intermediate energy demand, and a tax on intermediate uses combined with a reduction in intermediate energy demand. The methodology involves two versions of the input-output price model: a competitive price formulation and a mark-up price formulation. The input-output price framework will make it possible to evaluate how the alternative measures modify production prices, consumption prices, private real income, and intermediate energy uses. The empirical application is for the Catalan economy and uses economic data for the year 2001. The combination of a tax on energy uses and an improvement in the energy efficiency of the production system is a measure that accomplishes both economic and environmental goals, since it has no effects on prices, it has a positive effect on private real income and, finally, energy consumption is considerably reduced

  12. Aptamer-based analysis: a promising alternative for food safety control.

    Science.gov (United States)

    Amaya-González, Sonia; de-los-Santos-Alvarez, Noemí; Miranda-Ordieres, Arturo J; Lobo-Castañón, Maria Jesús

    2013-01-01

    Ensuring food safety is nowadays a top priority of authorities and professional players in the food supply chain. One of the key challenges to determine the safety of food and guarantee a high level of consumer protection is the availability of fast, sensitive and reliable analytical methods to identify specific hazards associated to food before they become a health problem. The limitations of existing methods have encouraged the development of new technologies, among them biosensors. Success in biosensor design depends largely on the development of novel receptors with enhanced affinity to the target, while being stable and economical. Aptamers fulfill these characteristics, and thus have surfaced as promising alternatives to natural receptors. This Review describes analytical strategies developed so far using aptamers for the control of pathogens, allergens, adulterants, toxins and other forbidden contaminants to ensure food safety. The main progresses to date are presented, highlighting potential prospects for the future. PMID:24287543

  13. Measurement and analysis of fission rate in alternate depleted uranium/polyethylene shells

    International Nuclear Information System (INIS)

    In order to check the conceptual design of the subcritical blanket in fusion-fission hybrid reactor, an integral experiment was carried out in alternate depleted uranium/polyethylene shells with 14 MeV neutron using activation technique. The 238U (n, f) and 235U (n, f) reaction rates at 90° direction to the incident D beam were determined by measuring the 293.3 keV γ ray emitted from 143Ce which is generated by 238U (n, f) and 235U (n, f) reactions. The experiment was simulated using MCNP5 code with ENDF/B-Ⅵ library, and the calculated 238U (n. f) and 235U (n, f) reaction rates are generally 5% higher than experimental results. (authors)

  14. Efficiency and exhaust gas analysis of variable compression ratio spark ignition engine fuelled with alternative fuels

    Directory of Open Access Journals (Sweden)

    N. Seshaiah

    2010-09-01

    Full Text Available Considering energy crises and pollution problems today, investigations have been concentrated on decreasing fuel consumption by using alternative fuels and on lowering the concentration of toxic components in combustion products. In the present work, the variable compression ratio spark ignition engine designed to run on gasoline has been tested with pure gasoline, LPG (Isobutene, and gasoline blended with ethanol 10%, 15%, 25% and 35% by volume. Also, the gasoline mixed with kerosene at 15%, 25% and 35% by volume without any engine modifications has been tested and presented the result. Brake thermal and volumetric efficiency variation with brake load is compared and presented. CO and CO2 emissions have been also compared for all tested fuels.

  15. Thermal degradation analysis of pongamia pinnata oil as alternative liquid dielectric for distribution transformer

    Indian Academy of Sciences (India)

    T MARIPRASATH; V KIRUBAKARAN

    2016-09-01

    In this paper the feasibility of non-edible pongamia pinnata oil (PPO) as an alternative liquid dielectric which can be used in distribution transformers is examined. Hence, electrical, physical and chemical properties have been measured for thermally aged (with and without catalytic added) pongamia pinnata oil (PPO) and mineral oil (MO), sampled at 110°C for 180 days (according to IEC and IS standard). The experimentresults show that, electrical properties of pongamia pinnata oil with catalytic (PPOWC) are better than those of mineral oil with catalytic (MOWC). Whereas, the viscosity and density of PPOWC sample is much higher at all sampling intervals, so that care must be taken for cooling tube design of transformer. Furthermore, for all aging period the total acid content in MOWC oil samples is very low compared to PPOWC. However, these are higher molecular weight acid which is beneficial than lower molecular weight acid generated by MOWC oil samples.

  16. 'Backscratching' alternative to PRSI pattern recognition. [position, rotation, and scale-independent image analysis

    Science.gov (United States)

    Monroe, Stanley E., Jr.; Juday, Richard D.

    1990-01-01

    The Backscratching optical correlation algorithm has been proposed for four degree of freedom tracking. In an alternating Cartesian and log-polar implementation, the tracked parameters are scale, rotation, and two-dimenisonal translation. The algorithm has a finite capture radius in the four-dimensional tracking space. The capture radius is dependent on the tracked object, the correlator architecture, and the method of filter computation. Some methods of extending the capture radius are discussed. One is a modification of matched filters, another is a careful consideration of log-polar transform center, and another is an operational method. Some simulations of the filter construction method, in which a larger capture radius is gained at the expense of precision in determining the four parameters are presented.

  17. Aptamer-Based Analysis: A Promising Alternative for Food Safety Control

    Directory of Open Access Journals (Sweden)

    Sonia Amaya-González

    2013-11-01

    Full Text Available Ensuring food safety is nowadays a top priority of authorities and professional players in the food supply chain. One of the key challenges to determine the safety of food and guarantee a high level of consumer protection is the availability of fast, sensitive and reliable analytical methods to identify specific hazards associated to food before they become a health problem. The limitations of existing methods have encouraged the development of new technologies, among them biosensors. Success in biosensor design depends largely on the development of novel receptors with enhanced affinity to the target, while being stable and economical. Aptamers fulfill these characteristics, and thus have surfaced as promising alternatives to natural receptors. This Review describes analytical strategies developed so far using aptamers for the control of pathogens, allergens, adulterants, toxins and other forbidden contaminants to ensure food safety. The main progresses to date are presented, highlighting potential prospects for the future.

  18. Aptamer-based analysis: a promising alternative for food safety control.

    Science.gov (United States)

    Amaya-González, Sonia; de-los-Santos-Alvarez, Noemí; Miranda-Ordieres, Arturo J; Lobo-Castañón, Maria Jesús

    2013-01-01

    Ensuring food safety is nowadays a top priority of authorities and professional players in the food supply chain. One of the key challenges to determine the safety of food and guarantee a high level of consumer protection is the availability of fast, sensitive and reliable analytical methods to identify specific hazards associated to food before they become a health problem. The limitations of existing methods have encouraged the development of new technologies, among them biosensors. Success in biosensor design depends largely on the development of novel receptors with enhanced affinity to the target, while being stable and economical. Aptamers fulfill these characteristics, and thus have surfaced as promising alternatives to natural receptors. This Review describes analytical strategies developed so far using aptamers for the control of pathogens, allergens, adulterants, toxins and other forbidden contaminants to ensure food safety. The main progresses to date are presented, highlighting potential prospects for the future.

  19. An empirical analysis on the adoption of alternative fuel vehicles: The case of natural gas vehicles

    International Nuclear Information System (INIS)

    The adoption of alternative fuel vehicles (AFVs) has been regarded as one of the most important strategies to address the issues of energy dependence, air quality, and, more recently, climate change. Despite decades of effort, we still face daunting challenges to promote wider acceptance of AFVs by the general public. More empirical analyses are needed to understand the technology adoption process associated with different market structures, the effectiveness of regulations and incentives, and the density of infrastructure adequate to reach sustainable commercial application. This paper compares the adoption of natural gas vehicles (NGVs) in eight countries: Argentina, Brazil, China, India, Italy, New Zealand, Pakistan, and the US. It examines the major policies aimed at promoting the use of NGVs, instruments for implementing those policies and targeting likely stakeholders, and a range of factors that influence the adoption of NGVs. The findings in this paper should be applicable to other AFVs

  20. PlantAPA: A Portal for Visualization and Analysis of Alternative Polyadenylation in Plants

    Science.gov (United States)

    Wu, Xiaohui; Zhang, Yumin; Li, Qingshun Q.

    2016-01-01

    Alternative polyadenylation (APA) is an important layer of gene regulation that produces mRNAs that have different 3′ ends and/or encode diverse protein isoforms. Up to 70% of annotated genes in plants undergo APA. Increasing numbers of poly(A) sites collected in various plant species demand new methods and tools to access and mine these data. We have created an open-access web service called PlantAPA (http://bmi.xmu.edu.cn/plantapa) to visualize and analyze genome-wide poly(A) sites in plants. PlantAPA provides various interactive and dynamic graphics and seamlessly integrates a genome browser that can profile heterogeneous cleavage sites and quantify expression patterns of poly(A) sites across different conditions. Particularly, through PlantAPA, users can analyze poly(A) sites in extended 3′ UTR regions, intergenic regions, and ambiguous regions owing to alternative transcription or RNA processing. In addition, it also provides tools for analyzing poly(A) site selections, 3′ UTR lengthening or shortening, non-canonical APA site switching, and differential gene expression between conditions, making it more powerful for the study of APA-mediated gene expression regulation. More importantly, PlantAPA offers a bioinformatics pipeline that allows users to upload their own short reads or ESTs for poly(A) site extraction, enabling users to further explore poly(A) site selection using stored PlantAPA poly(A) sites together with their own poly(A) site datasets. To date, PlantAPA hosts the largest database of APA sites in plants, including Oryza sativa, Arabidopsis thaliana, Medicago truncatula, and Chlamydomonas reinhardtii. As a user-friendly web service, PlantAPA will be a valuable addition to the community of biologists studying APA mechanisms and gene expression regulation in plants. PMID:27446120

  1. Determining the Number of Principal Components to Retain via Parallel Analysis: Alternatives to Monte Carlo Analyses.

    Science.gov (United States)

    Lautenschlager, Gary J.

    The parallel analysis method for determining the number of components to retain in a principal components analysis has received a recent resurgence of support and interest. However, researchers and practitioners desiring to use this criterion have been hampered by the required Monte Carlo analyses needed to develop the criteria. Two recent…

  2. Analysis of removal alternatives for the Heavy Water Components Test Reactor at the Savannah River Site. Revision 1

    Energy Technology Data Exchange (ETDEWEB)

    Owen, M.B.

    1997-04-01

    This engineering study evaluates different alternatives for decontamination and decommissioning of the Heavy Water Components Test Reactor (HWCTR). Cooled and moderated with pressurized heavy water, this uranium-fueled nuclear reactor was designed to test fuel assemblies for heavy water power reactors. It was operated for this purpose from march of 1962 until December of 1964. Four alternatives studied in detail include: (1) dismantlement, in which all radioactive and hazardous contaminants would be removed, the containment dome dismantled and the property restored to a condition similar to its original preconstruction state; (2) partial dismantlement and interim safe storage, where radioactive equipment except for the reactor vessel and steam generators would be removed, along with hazardous materials, and the building sealed with remote monitoring equipment in place to permit limited inspections at five-year intervals; (3) conversion for beneficial reuse, in which most radioactive equipment and hazardous materials would be removed and the containment building converted to another use such as a storage facility for radioactive materials, and (4) entombment, which involves removing hazardous materials, filling the below-ground structure with concrete, removing the containment dome and pouring a concrete cap on the tomb. Also considered was safe storage, but this approach, which has, in effect, been followed for the past 30 years, did not warrant detailed evaluation. The four other alternatives were evaluate, taking into account factors such as potential effects on the environment, risks, effectiveness, ease of implementation and cost. The preferred alternative was determined to be dismantlement. This approach is recommended because it ranks highest in the comparative analysis, would serve as the best prototype for the site reactor decommissioning program and would be most compatible with site property reuse plans for the future.

  3. Analysis of removal alternatives for the Heavy Water Components Test Reactor at the Savannah River Site. Revision 1

    International Nuclear Information System (INIS)

    This engineering study evaluates different alternatives for decontamination and decommissioning of the Heavy Water Components Test Reactor (HWCTR). Cooled and moderated with pressurized heavy water, this uranium-fueled nuclear reactor was designed to test fuel assemblies for heavy water power reactors. It was operated for this purpose from march of 1962 until December of 1964. Four alternatives studied in detail include: (1) dismantlement, in which all radioactive and hazardous contaminants would be removed, the containment dome dismantled and the property restored to a condition similar to its original preconstruction state; (2) partial dismantlement and interim safe storage, where radioactive equipment except for the reactor vessel and steam generators would be removed, along with hazardous materials, and the building sealed with remote monitoring equipment in place to permit limited inspections at five-year intervals; (3) conversion for beneficial reuse, in which most radioactive equipment and hazardous materials would be removed and the containment building converted to another use such as a storage facility for radioactive materials, and (4) entombment, which involves removing hazardous materials, filling the below-ground structure with concrete, removing the containment dome and pouring a concrete cap on the tomb. Also considered was safe storage, but this approach, which has, in effect, been followed for the past 30 years, did not warrant detailed evaluation. The four other alternatives were evaluate, taking into account factors such as potential effects on the environment, risks, effectiveness, ease of implementation and cost. The preferred alternative was determined to be dismantlement. This approach is recommended because it ranks highest in the comparative analysis, would serve as the best prototype for the site reactor decommissioning program and would be most compatible with site property reuse plans for the future

  4. Elusive Critical Elements of Transformative Risk Assessment Practice and Interpretation: Is Alternatives Analysis the Next Step?

    Science.gov (United States)

    Francis, Royce A

    2015-11-01

    This article argues that "game-changing" approaches to risk analysis must focus on "democratizing" risk analysis in the same way that information technologies have democratized access to, and production of, knowledge. This argument is motivated by the author's reading of Goble and Bier's analysis, "Risk Assessment Can Be a Game-Changing Information Technology-But Too Often It Isn't" (Risk Analysis, 2013; 33: 1942-1951), in which living risk assessments are shown to be "game changing" in probabilistic risk analysis. In this author's opinion, Goble and Bier's article focuses on living risk assessment's potential for transforming risk analysis from the perspective of risk professionals-yet, the game-changing nature of information technologies has typically achieved a much broader reach. Specifically, information technologies change who has access to, and who can produce, information. From this perspective, the author argues that risk assessment is not a game-changing technology in the same way as the printing press or the Internet because transformative information technologies reduce the cost of production of, and access to, privileged knowledge bases. The author argues that risk analysis does not reduce these costs. The author applies Goble and Bier's metaphor to the chemical risk analysis context, and in doing so proposes key features that transformative risk analysis technology should possess. The author also discusses the challenges and opportunities facing risk analysis in this context. These key features include: clarity in information structure and problem representation, economical information dissemination, increased transparency to nonspecialists, democratized manufacture and transmission of knowledge, and democratic ownership, control, and interpretation of knowledge. The chemical safety decision-making context illustrates the impact of changing the way information is produced and accessed in the risk context. Ultimately, the author concludes that although

  5. High resolution analysis of the human transcriptome: detection of extensive alternative splicing independent of transcriptional activity

    Directory of Open Access Journals (Sweden)

    Rouet Fabien

    2009-10-01

    Full Text Available Abstract Background Commercially available microarrays have been used in many settings to generate expression profiles for a variety of applications, including target selection for disease detection, classification, profiling for pharmacogenomic response to therapeutics, and potential disease staging. However, many commercially available microarray platforms fail to capture transcript diversity produced by alternative splicing, a major mechanism for driving proteomic diversity through transcript heterogeneity. Results The human Genome-Wide SpliceArray™ (GWSA, a novel microarray platform, utilizes an existing probe design concept to monitor such transcript diversity on a genome scale. The human GWSA allows the detection of alternatively spliced events within the human genome through the use of exon body and exon junction probes to provide a direct measure of each transcript, through simple calculations derived from expression data. This report focuses on the performance and validation of the array when measured against standards recently published by the Microarray Quality Control (MAQC Project. The array was shown to be highly quantitative, and displayed greater than 85% correlation with the HG-U133 Plus 2.0 array at the gene level while providing more extensive coverage of each gene. Almost 60% of splice events among genes demonstrating differential expression of greater than 3 fold also contained extensive splicing alterations. Importantly, almost 10% of splice events within the gene set displaying constant overall expression values had evidence of transcript diversity. Two examples illustrate the types of events identified: LIM domain 7 showed no differential expression at the gene level, but demonstrated deregulation of an exon skip event, while erythrocyte membrane protein band 4.1 -like 3 was differentially expressed and also displayed deregulation of a skipped exon isoform. Conclusion Significant changes were detected independent of

  6. An alternative method for the analysis of neuron passive electrical data which uses integrals of voltage transients.

    Science.gov (United States)

    Engelhardt, J K; Morales, F R; Chase, M H

    1998-06-01

    The traditional method for analyzing passive electrical data from neurons when specific morphological data are unavailable consists of decomposing the voltage response of the cell into a series of exponential functions (the peeling method) and substituting the time constants of these exponential functions into equations derived from cable theory (Rall W, Core conductor theory and cable properties of neurons. In: Handbook of Physiology. The Nervous System. Cellular Biology of Neurons. Bethesda, MD. Am Physiol Soc. Section 1, Part 1, 1977;1(3):39-97). In the present report, an alternative method is examined for analyzing these kinds of data, the integrals of transients method (Eisenberg RS, Mathias RT. Structural analysis of electrical properties of cells and tissues. CRC Critical Reviews in Bioengineering 1980;4:203-232). The integrals required are easily obtained from input resistance data and any theoretical model that is appropriate for the neurons under study can be used, provided that the impedance function can be determined. In order to demonstrate this alternative method, a simple 3-compartment model with both dendritic taper and somatic shunt is used to model data obtained from fast-type alpha-motoneurons in the spinal cord of the cat. These results are compared with results obtained using the traditional peeling method. This comparison indicates that passive electrical data from fast-type motoneurons are best analyzed using a theoretical model that includes both dendritic taper and somatic shunt. Furthermore, our results show that the integrals of transients method can facilitate this analysis. PMID:9696318

  7. Analysis of the use of biomass as an energy alternative for the Portuguese textile dyeing industry

    International Nuclear Information System (INIS)

    The energy efficiency and the development of environmentally correct policies are current topics, especially when applied to the industrial sector with the objective of increasing the competitiveness of the enterprises. Portuguese textile dyeing sector, being a major consumer sector of primary energy, needs to adopt measures to improve its competitiveness. Biomass appears to be a viable and preferred alternative energy source for the sector, while simultaneously develops an entire forest industry devoted to the supply of forest solid fuels. This work carries out a comprehensive PEST (political, economic, social and technological) analysis, which analyses Political, Economic, Social and Technological aspects of the replacement of the fossil fuels traditionally used in this sector by biomass, providing a framework of environmental factors that influence the strategic management of the companies. The main advantages are the reduction of external dependence on imported fuel due to the use of an endogenous renewable resource, the creation and preservation of jobs, the increased competitiveness of the sector by reducing energy costs, the use of national technology and the reduction of greenhouse gases emissions. - Highlights: • The Portuguese textile dyeing sector, being a major consumer sector of primary energy, is addressed. • Biomass is a viable and preferred alternative energy source for the sector. • A PEST (political, economic, social and technological) analysis is carried out. • The implications of the replacement of fossil fuels with biomass are studied

  8. Alternative splicing of human elastin mRNA indicated by sequence analysis of cloned genomic and complementary DNA

    International Nuclear Information System (INIS)

    Poly(A)+ RNA, isolated from a single 7-mo fetal human aorta, was used to synthesize cDNA by the RNase H method, and the cDNA was inserted into λgt10. Recombinant phage containing elastin sequences were identified by hybridization with cloned, exon-containing fragments of the human elastin gene. Three clones containing inserts of 3.3, 2.7, and 2.3 kilobases were selected for further analysis. Three overlapping clones containing 17.8 kilobases of the human elastin gene were also isolated from genomic libraries. Complete sequence analysis of the six clones demonstrated that: (i) the cDNA encompassed the entire translated portion of the mRNA encoding 786 amino acids, including several unusual hydrophilic amino acid sequences not previously identified in porcine tropoelastin, (ii) exons encoding either hydrophobic or crosslinking domains in the protein alternated in the gene, and (iii) a great abundance of Alu repetitive sequences occurred throughout the introns. The data also indicated substantial alternative splicing of the mRNA. These results suggest the potential for significant variation in the precise molecular structure of the elastic fiber in the human population

  9. Electric Demand Analysis of the Tunisian Network: Trends and Short Term Photovoltaic Implementation with Alternatives Prospects and Technologies

    Directory of Open Access Journals (Sweden)

    J. Bouattour

    2014-08-01

    Full Text Available The main objective of this study is to analyze the system of electric demand in Tunisia and to propose immediate alternative solution of photovoltaic implementation that can be suitable based on available natural solar energy resources in the country to respond to the short Forecast peak demand growth and energy consumptions taking into account financial strain. On the first step, analysis of the situation based on the recent historical data is proposed. In a second step, a solution is considered, based on photovoltaic implementation and using the results of historical data analysis. The resulting benefits are highlighted: financial, technical, environmental and social. Future studies may be made to use a mix of technology and policy as well: among it combining photovoltaic and battery storage, in parallel with energy efficiency programs.

  10. Scaling analysis of heart beat fluctuations data and its relationship with cyclic alternating pattern data during sleep

    Science.gov (United States)

    de León-Lomelí, R.; Murguía, J. S.; Chouvarda, I.; Méndez, M. O.; González-Galván, E.; Alba, A.

    2016-01-01

    During sleep there exists a nonlinear dynamic phenomenon, which is called cyclic alternating pattern. This phenomenon is generated in the brain and is composed of a series of events of short duration known as A-phases. It has been shown that A-phases can be found in other physiological systems such as the cardiovascular. However, there is no evidence that shows the temporal influence of the A-phases with the cardiovascular system. For this purpose, we consider the scaling method known as detrended fluctuation analysis (DFA). The analysis was carried out in well sleepers and insomnia people, and the numerical results show an increment in the scaling parameter for the insomnia subjects compared with the normal ones. In addition, the results of the heart dynamics suggests a persistent behavior toward the 1/f-noise.

  11. Hemp Thermal Insulation Concrete with Alternative Binders, Analysis of their Thermal and Mechanical Properties

    Science.gov (United States)

    Sinka, M.; Sahmenko, G.; Korjakins, A.; Radina, L.; Bajare, D.

    2015-11-01

    One of the main challenges that construction industry faces today is how to address the demands for more sustainable, environmentally friendly and carbon neutral construction materials and building upkeep processes. One of the answers to these demands is lime-hemp concrete (LHC) building materials - carbon negative materials that have sufficient thermal insulation capabilities to be used as thermal insulation materials for new as well as for existing buildings. But one problem needs to be overcome before these materials can be used on a large scale - current manufacturing technology allows these materials to be used only as self-bearing thermal insulation material with large labour intensity in the manufacturing process. In order to lower the labour intensity and allow the material to be used in wider applications, a LHC block and board production is necessary, which in turn calls for the binders different from the classically used ones, as they show insufficient mechanical strength for this new use. The particular study focuses on alternative binders produced using gypsum-cement compositions ensuring they are usable in outdoor applications together with hemp shives. Physical, mechanical, thermal and water absorption properties of hemp concrete with various binders are addressed in the current study.

  12. Economic Analysis of Alternative Strategies for Detection of ALK Rearrangements in Non Small Cell Lung Cancer

    Directory of Open Access Journals (Sweden)

    Shivang Doshi

    2016-01-01

    Full Text Available Identification of alterations in ALK gene and development of ALK-directed therapies have increased the need for accurate and efficient detection methodologies. To date, research has focused on the concordance between the two most commonly used technologies, fluorescent in situ hybridization (FISH and immunohistochemistry (IHC. However, inter-test concordance reflects only one, albeit important, aspect of the diagnostic process; laboratories, hospitals, and payors must understand the cost and workflow of ALK rearrangement detection strategies. Through literature review combined with interviews of pathologists and laboratory directors in the U.S. and Europe, a cost-impact model was developed that compared four alternative testing strategies—IHC only, FISH only, IHC pre-screen followed by FISH confirmation, and parallel testing by both IHC and FISH. Interviews were focused on costs of reagents, consumables, equipment, and personnel. The resulting model showed that testing by IHC alone cost less ($90.07 in the U.S., $68.69 in Europe than either independent or parallel testing by both FISH and IHC ($441.85 in the U.S. and $279.46 in Europe. The strategies differed in cost of execution, turnaround time, reimbursement, and number of positive results detected, suggesting that laboratories must weigh the costs and the clinical benefit of available ALK testing strategies.

  13. Analysis of current and alternative phenol based RNA extraction methodologies for cyanobacteria

    Directory of Open Access Journals (Sweden)

    Lindblad Peter

    2009-08-01

    Full Text Available Abstract Background The validity and reproducibility of gene expression studies depend on the quality of extracted RNA and the degree of genomic DNA contamination. Cyanobacteria are gram-negative prokaryotes that synthesize chlorophyll a and carry out photosynthetic water oxidation. These organisms possess an extended array of secondary metabolites that impair cell lysis, presenting particular challenges when it comes to nucleic acid isolation. Therefore, we used the NHM5 strain of Nostoc punctiforme ATCC 29133 to compare and improve existing phenol based chemistry and procedures for RNA extraction. Results With this work we identify and explore strategies for improved and lower cost high quality RNA isolation from cyanobacteria. All the methods studied are suitable for RNA isolation and its use for downstream applications. We analyse different Trizol based protocols, introduce procedural changes and describe an alternative RNA extraction solution. Conclusion It was possible to improve purity of isolated RNA by modifying protocol procedures. Further improvements, both in RNA purity and experimental cost, were achieved by using a new extraction solution, PGTX.

  14. In vitro determination of the indigestible fraction in foods: an alternative to dietary fiber analysis.

    Science.gov (United States)

    Saura-Calixto, F; García-Alonso, A; Goñi, I; Bravo, L

    2000-08-01

    Dietary fiber (DF) intakes in Western countries only accounts for about one-third of the substrates required for colonic bacterial cell turnover. There is a general trend among nutritionists to extend the DF concept to include all food constituents reaching the colon. In this line, a method to quantify the major nondigestible components in plant foods, namely, the indigestible fraction (IF), is presented. Analytical conditions for IF determination are close to physiological. Samples, analyzed as eaten, were successively incubated with pepsin and alpha-amylase; after centrifugation and dialysis, insoluble and soluble IFs were obtained. IF values include DF, resistant starch, resistant protein, and other associated compounds. IF contents determined in common foods (cereals, legumes, vegetables, and fruits) were higher than DF contents. Calculated IF intakes were close to the estimated amount of substrates reaching the colon. IF data could be more useful than DF data from a nutritional point of view; therefore, IF is proposed as an alternative to DF for food labeling and food composition tables. PMID:10956113

  15. A COMPARATIVE ANALYSIS BETWEEN UNIT-LINKED LIFE INSURANCE AND OTHER ALTERNATIVE INVESTMENTS

    Directory of Open Access Journals (Sweden)

    CRISTINA CIUMAS

    2015-07-01

    Full Text Available The unit-linked life insurance has two important components: protection and investment. The protection component refers to the insured sum in case of the occurrence of insured risks and the investment component refers to the policyholder’s account that represents the present value of the units from the chosen investment funds.These financial products invest most of the premium paid by the insured person in the funds managed by the insurance company or an external administrator and the lower part of the premium is intended to cover the insured risk (death, disability, etc. An important component of the activity carried out by the insurance companies is the investment of the premiums paid by policyholders in various types of assets, in order to obtain higher yields than those guaranteed by the insurance contracts, while providing the necessary liquidity for the payment of insurance claims in case of occurrence of the assumed risks. This research contributes to the existing literature regarding the study of investment alternatives, with an exclusive focus on the investment in unit-linked life insurance. A special place in this study is the presentation of investments in unit-linked insurance versus other types of financial investments: deposits, treasury bills, shares (BET, currency (EURO and gold.

  16. Multi-dimensional scavenging analysis of a free-piston linear alternator based on numerical simulation

    Energy Technology Data Exchange (ETDEWEB)

    Mao, Jinlong; Zuo, Zhengxing; Li, Wen; Feng, Huihua [School of Mechanical Engineering, Beijing Institute of Technology, Beijing 100081 (China)

    2011-04-15

    A free-piston linear alternator (FPLA) is being developed by the Beijing Institute of Technology to improve the thermal efficiency relative to conventional crank-driven engines. A two-stroke scavenging process recharges the engine and is crucial to realizing the continuous operation of a free-piston engine. In order to study the FPLA scavenging process, the scavenging system was configured using computational fluid dynamics. As the piston dynamics of the FPLA are different to conventional crank-driven two-stroke engines, a time-based numerical simulation program was built using Matlab to define the piston's motion profiles. A wide range of design and operating options were investigated including effective stroke length, valve overlapping distance, operating frequency and charging pressure to find out their effects on the scavenging performance. The results indicate that a combination of high effective stroke length to bore ratio and long valve overlapping distance with a low supercharging pressure has the potential to achieve high scavenging and trapping efficiencies with low short-circuiting losses. (author)

  17. Economic Analysis of Alternative Strategies for Detection of ALK Rearrangements in Non Small Cell Lung Cancer.

    Science.gov (United States)

    Doshi, Shivang; Ray, David; Stein, Karen; Zhang, Jie; Koduru, Prasad; Fogt, Franz; Wellman, Axel; Wat, Ricky; Mathews, Charles

    2016-01-01

    Identification of alterations in ALK gene and development of ALK-directed therapies have increased the need for accurate and efficient detection methodologies. To date, research has focused on the concordance between the two most commonly used technologies, fluorescent in situ hybridization (FISH) and immunohistochemistry (IHC). However, inter-test concordance reflects only one, albeit important, aspect of the diagnostic process; laboratories, hospitals, and payors must understand the cost and workflow of ALK rearrangement detection strategies. Through literature review combined with interviews of pathologists and laboratory directors in the U.S. and Europe, a cost-impact model was developed that compared four alternative testing strategies-IHC only, FISH only, IHC pre-screen followed by FISH confirmation, and parallel testing by both IHC and FISH. Interviews were focused on costs of reagents, consumables, equipment, and personnel. The resulting model showed that testing by IHC alone cost less ($90.07 in the U.S., $68.69 in Europe) than either independent or parallel testing by both FISH and IHC ($441.85 in the U.S. and $279.46 in Europe). The strategies differed in cost of execution, turnaround time, reimbursement, and number of positive results detected, suggesting that laboratories must weigh the costs and the clinical benefit of available ALK testing strategies. PMID:26838801

  18. Comparative cost analysis -- computed tomography vs. alternative diagnostic procedures, 1977-1980

    International Nuclear Information System (INIS)

    In comparing the total national cost of utilizing computed tomography (CT) for medically indicated diagnoses with that of conventional x-ray, ultrasonography, nuclear medicine, and exploratory surgery, this investigation concludes that there was little, if any, added net cost from CT use in 1977 or will there be in 1980. Computed tomography, generally recognized as a reliable and useful diagnostic modality, has the potential to reduce net costs provided that an optimal number of units can be made available to physicians and patients to achieve projected reductions in alternative procedures. This study examines the actual cost impact of CT on both cranial and body diagnostic procedures. For abdominal and mediastinal disorders, CT scanning is just beginning to emerge as a diagnostic modality. As such, clinical experience is somewhat limited and the authors assume that no significant reduction in conventional procedures took place in 1977. It is estimated that the approximately 375,000 CT body procedures performed in 1977 represent only a 5 percent cost increase over use of other diagnostic modalities. It is projected that 2,400,000 CT body procedures will be performed in 1980 and, depending on assumptions used, total body diagnostic costs will increase only slightly or be reduced. Thirty-one tables appear throughout the text presenting cost data broken down by types of diagnostic procedures used and projections by years. Appendixes present technical cost components for diagnostic procedures, the comparative efficacy of CT as revealed in abstracts of published literature, selected medical diagnoses, and references

  19. Economical analysis of an alternative strategy for CO2 mitigation based on nuclear power

    International Nuclear Information System (INIS)

    Many countries are pursuing greenhouse gas (GHG) mitigation policies resulting in the increase of use of renewable sources in the electricity sector to mitigate CO2 emissions. Nuclear energy is a non-emitting CO2 source that could be used as part of that policy. However, its main drawback is the high investment required for its deployment. On the other hand, wind power is the clean source preferred option to mitigate CO2 emissions. However, due to its intermittence backup power is needed, in most of the cases it must be provided with combined cycle thermal plants using natural gas. This study performs an economical comparison of a hypothetical implementation of a nuclear strategy to meet the same CO2 emissions reduction goal that has been obtained by the actual Spaniard strategy (2005–2010) based on wind power. The investment required in both strategies is assessed under different investment scenarios and electricity production conditions for nuclear power. Also, the cost of electricity generation is compared for both strategies. - Highlights: ► Wind power electricity cost including its backup in Spain is assessed. ► Nuclear power is proposed as an alternative to produce the same CO2 reduction. ► Nuclear power requires less installed capacity deployment. ► Investment to produce the same CO2 reduction is smaller using nuclear power. ► Electricity generating cost is less expensive using the nuclear option

  20. Integrative Genome-wide Analysis Reveals Cooperative Regulation of Alternative Splicing by hnRNP Proteins

    Directory of Open Access Journals (Sweden)

    Stephanie C. Huelga

    2012-02-01

    Full Text Available Understanding how RNA binding proteins control the splicing code is fundamental to human biology and disease. Here, we present a comprehensive study to elucidate how heterogeneous nuclear ribonucleoparticle (hnRNP proteins, among the most abundant RNA binding proteins, coordinate to regulate alternative pre-mRNA splicing (AS in human cells. Using splicing-sensitive microarrays, crosslinking and immunoprecipitation coupled with high-throughput sequencing (CLIP-seq, and cDNA sequencing, we find that more than half of all AS events are regulated by multiple hnRNP proteins and that some combinations of hnRNP proteins exhibit significant synergy, whereas others act antagonistically. Our analyses reveal position-dependent RNA splicing maps, in vivo consensus binding sites, a surprising level of cross- and autoregulation among hnRNP proteins, and the coordinated regulation by hnRNP proteins of dozens of other RNA binding proteins and genes associated with cancer. Our findings define an unprecedented degree of complexity and compensatory relationships among hnRNP proteins and their splicing targets that likely confer robustness to cells.

  1. Alternative method of highway traffic safety analysis for developing countries using delphi technique and Bayesian network.

    Science.gov (United States)

    Mbakwe, Anthony C; Saka, Anthony A; Choi, Keechoo; Lee, Young-Jae

    2016-08-01

    Highway traffic accidents all over the world result in more than 1.3 million fatalities annually. An alarming number of these fatalities occurs in developing countries. There are many risk factors that are associated with frequent accidents, heavy loss of lives, and property damage in developing countries. Unfortunately, poor record keeping practices are very difficult obstacle to overcome in striving to obtain a near accurate casualty and safety data. In light of the fact that there are numerous accident causes, any attempts to curb the escalating death and injury rates in developing countries must include the identification of the primary accident causes. This paper, therefore, seeks to show that the Delphi Technique is a suitable alternative method that can be exploited in generating highway traffic accident data through which the major accident causes can be identified. In order to authenticate the technique used, Korea, a country that underwent similar problems when it was in its early stages of development in addition to the availability of excellent highway safety records in its database, is chosen and utilized for this purpose. Validation of the methodology confirms the technique is suitable for application in developing countries. Furthermore, the Delphi Technique, in combination with the Bayesian Network Model, is utilized in modeling highway traffic accidents and forecasting accident rates in the countries of research. PMID:27183516

  2. Tumor-specific usage of alternative transcription start sites in colorectal cancer identified by genome-wide exon array analysis

    Directory of Open Access Journals (Sweden)

    Laurila Kirsti

    2011-10-01

    Full Text Available Abstract Background Approximately half of all human genes use alternative transcription start sites (TSSs to control mRNA levels and broaden the transcriptional output in healthy tissues. Aberrant expression patterns promoting carcinogenesis, however, may arise from alternative promoter usage. Results By profiling 108 colorectal samples using exon arrays, we identified nine genes (TCF12, OSBPL1A, TRAK1, ANK3, CHEK1, UGP2, LMO7, ACSL5, and SCIN showing tumor-specific alternative TSS usage in both adenoma and cancer samples relative to normal mucosa. Analysis of independent exon array data sets corroborated these findings. Additionally, we confirmed the observed patterns for selected mRNAs using quantitative real-time reverse-transcription PCR. Interestingly, for some of the genes, the tumor-specific TSS usage was not restricted to colorectal cancer. A comprehensive survey of the nine genes in lung, bladder, liver, prostate, gastric, and brain cancer revealed significantly altered mRNA isoform ratios for CHEK1, OSBPL1A, and TCF12 in a subset of these cancer types. To identify the mechanism responsible for the shift in alternative TSS usage, we antagonized the Wnt-signaling pathway in DLD1 and Ls174T colorectal cancer cell lines, which remarkably led to a shift in the preferred TSS for both OSBPL1A and TRAK1. This indicated a regulatory role of the Wnt pathway in selecting TSS, possibly also involving TP53 and SOX9, as their transcription binding sites were enriched in the promoters of the tumor preferred isoforms together with their mRNA levels being increased in tumor samples. Finally, to evaluate the prognostic impact of the altered TSS usage, immunohistochemistry was used to show deregulation of the total protein levels of both TCF12 and OSBPL1A, corresponding to the mRNA levels observed. Furthermore, the level of nuclear TCF12 had a significant correlation to progression free survival in a cohort of 248 stage II colorectal cancer samples

  3. Warranty cost analysis using alternating quasi-renewal processes with a warranty option

    Science.gov (United States)

    Yedida, Sarada; Unnissa Munavar, Mubashir; Ranjani, R.

    2012-03-01

    This article analyses a repairable deteriorating system with quasi-renewal operating and repair times during warranty. To establish the importance of non-negligible repair times modelled with quasi-renewal processes in warranty cost analysis, a fixed warranty model is developed and the results are compared with an existing model using expected warranty cost. Sensitivity analysis and graphical illustrations are provided to highlight the effect of various cost parameters on the expected warranty cost by means of three different distributions. In order to examine the cost implications to the customer and manufacturer, an extended warranty model in which a manufacturer offers a warranty option to the customer with two different policies, has been proposed. Based on the long-run average cost per unit time, profit analysis has been carried out for the manufacturer as it is an essential aspect of warranty management. This article emphasises the incorporation of non-negligible 'improved' repair times during warranty.

  4. ALTERNATIVE METHODS TO MULTIPLE CORRESPONDENCE ANALYSIS IN RECONSTRUCTING THE RELEVANT INFORMATION IN A BURT'S TABLE

    Directory of Open Access Journals (Sweden)

    Sergio Camiz

    2016-04-01

    Full Text Available ABSTRACT In this work, the reconstruction of the Burt's table, Greenacre (1988's Joint Correspondence Analysis (JCA, and Gower & Hand (1996's Extended Matching Coefficient (EMC are compared to Multiple Correspondence Analysis (MCA in order to check the quality of the methods. In particular, for the whole table, the ability is considered separately the diagonal, and the off-diagonal tables, that is the ability to describe either each character's distribution or the interaction between pairs of characters, or both. The theoretical aspects are discussed first, and finally the results obtained in an application are shown and discussed.

  5. Comparative analysis indicates that alternative splicing in plants has a limited role in functional expansion of the proteome

    Directory of Open Access Journals (Sweden)

    Stiekema Willem J

    2009-04-01

    Full Text Available Abstract Background Alternative splicing (AS is a widespread phenomenon in higher eukaryotes but the extent to which it leads to functional protein isoforms and to proteome expansion at large is still a matter of debate. In contrast to animal species, for which AS has been studied extensively at the protein and functional level, protein-centered studies of AS in plant species are scarce. Here we investigate the functional impact of AS in dicot and monocot plant species using a comparative approach. Results Detailed comparison of AS events in alternative spliced orthologs from the dicot Arabidopsis thaliana and the monocot Oryza sativa (rice revealed that the vast majority of AS events in both species do not result from functional conservation. Transcript isoforms that are putative targets for the nonsense-mediated decay (NMD pathway are as likely to contain conserved AS events as isoforms that are translated into proteins. Similar results were obtained when the same comparison was performed between the two more closely related monocot species rice and Zea mays (maize. Genome-wide computational analysis of functional protein domains encoded in alternatively and constitutively spliced genes revealed that only the RNA recognition motif (RRM is overrepresented in alternatively spliced genes in all species analyzed. In contrast, three domain types were overrepresented in constitutively spliced genes. AS events were found to be less frequent within than outside predicted protein domains and no domain type was found to be enriched with AS introns. Analysis of AS events that result in the removal of complete protein domains revealed that only a small number of domain types is spliced-out in all species analyzed. Finally, in a substantial fraction of cases where a domain is completely removed, this domain appeared to be a unit of a tandem repeat. Conclusion The results from the ortholog comparisons suggest that the ability of a gene to produce more than

  6. Complementary and alternative medicine use among older Australian women - a qualitative analysis

    Directory of Open Access Journals (Sweden)

    McLaughlin Deirdre

    2012-04-01

    Full Text Available Abstract Background The use of complementary and alternative medicines (CAM among older adults is an emerging health issue, however little is known about older people's experiences of using CAM and the cultural, geographical and other determinants of CAM use in this population. This study used qualitative methods to explore older women's views of CAM and reasons for their use of CAM. Participants for the project were drawn from the Australian Longitudinal Study on Women's Health (ALSWH 1921-1926 birth cohort. Women who responded positively to a question about CAM use in Survey 5 (2008 of the ALSWH were invited to participate in the study. A total of 13 rural and 12 urban women aged between 83 and 88 years agreed to be interviewed. Results The women expressed a range of views on CAM which fell into three broad themes: "push" factors such as dissatisfaction with conventional health services, "pull" factors which emphasised the positive aspects of choice and self-care in health matters, and barriers to CAM use. Overall, the "push' factors did not play a major role in the decision to use CAM, rather this was driven by "pull" factors related to health care self-responsibility and being able to source positive information about types of CAM. A number of barriers were identified such as access difficulties associated with increased age, limited mobility and restricted transport options, as well as financial constraints. Conclusions CAM use among older women was unlikely to be influenced by aspects of conventional health care ("push factors", but rather was reflective of the personal beliefs of the women and members of their close social networks ("pull factors". While it was also apparent that there were differences between the rural and urban women in their use of CAM, the reasons for this were mainly due to the difficulties inherent in accessing certain types of CAM in rural areas.

  7. Transcriptomic analysis of human polarized macrophages: more than one role of alternative activation?

    Directory of Open Access Journals (Sweden)

    Eleonora Derlindati

    Full Text Available Macrophages are a heterogeneous cell population which in response to the cytokine milieu polarize in either classically activated macrophages (M1 or alternatively activated macrophages (M2. This plasticity makes macrophages essential in regulating inflammation, immune response and tissue remodeling and a novel therapeutic target in inflammatory diseases such as atherosclerosis. The aim of the study was to describe the transcriptomic profiles of differently polarized human macrophages to generate new hypotheses on the biological function of the different macrophage subtypes.Polarization of circulating monocytes/macrophages of blood donors was induced in vitro by IFN-γ and LPS (M1, by IL-4 (M2a, and by IL-10 (M2c. Unstimulated cells (RM served as time controls. Gene expression profile of M1, M2a, M2c and RM was assessed at 6, 12 and 24h after polarization with Whole Human Genome Agilent Microarray technique. When compared to RM, M1 significantly upregulated pathways involved in immunity and inflammation, whereas M2a did the opposite. Conversely, decreased and increased expression of mitochondrial metabolism, consistent with insulin resistant and insulin sensitive patterns, was seen in M1 and M2a, respectively. The time sequence in the expression of some pathways appeared to have some specific bearing on M1 function. Finally, canonical and non-canonical Wnt genes and gene groups, promoting inflammation and tissue remodeling, were upregulated in M2a compared to RM.Our data in in vitro polarized human macrophages: 1. confirm and extend known inflammatory and anti-inflammatory gene expression patterns; 2. demonstrate changes in mitochondrial metabolism associated to insulin resistance and insulin sensitivity in M1 and M2a, respectively; 3. highlight the potential relevance of gene expression timing in M1 function; 4. unveil enhanced expression of Wnt pathways in M2a suggesting a potential dual (pro-inflammatory and anti-inflammatory role of M2a in

  8. Non-homologous isofunctional enzymes: A systematic analysis of alternative solutions in enzyme evolution

    Directory of Open Access Journals (Sweden)

    Wolf Yuri I

    2010-04-01

    Full Text Available Abstract Background Evolutionarily unrelated proteins that catalyze the same biochemical reactions are often referred to as analogous - as opposed to homologous - enzymes. The existence of numerous alternative, non-homologous enzyme isoforms presents an interesting evolutionary problem; it also complicates genome-based reconstruction of the metabolic pathways in a variety of organisms. In 1998, a systematic search for analogous enzymes resulted in the identification of 105 Enzyme Commission (EC numbers that included two or more proteins without detectable sequence similarity to each other, including 34 EC nodes where proteins were known (or predicted to have distinct structural folds, indicating independent evolutionary origins. In the past 12 years, many putative non-homologous isofunctional enzymes were identified in newly sequenced genomes. In addition, efforts in structural genomics resulted in a vastly improved structural coverage of proteomes, providing for definitive assessment of (nonhomologous relationships between proteins. Results We report the results of a comprehensive search for non-homologous isofunctional enzymes (NISE that yielded 185 EC nodes with two or more experimentally characterized - or predicted - structurally unrelated proteins. Of these NISE sets, only 74 were from the original 1998 list. Structural assignments of the NISE show over-representation of proteins with the TIM barrel fold and the nucleotide-binding Rossmann fold. From the functional perspective, the set of NISE is enriched in hydrolases, particularly carbohydrate hydrolases, and in enzymes involved in defense against oxidative stress. Conclusions These results indicate that at least some of the non-homologous isofunctional enzymes were recruited relatively recently from enzyme families that are active against related substrates and are sufficiently flexible to accommodate changes in substrate specificity. Reviewers This article was reviewed by Andrei

  9. A Suggested Approach for a Cost Analysis of Alternative IMS Configurations.

    Science.gov (United States)

    Besel, Ronald

    An approach to a cost analysis of pilot, prototype, model and operational versions of the Southwest Regional Laboratory's (SWRL) Instructional Management System (IMS) is described. Five classes of systems (conceptual, pilot, prototype, model and operational) are defined, and a system development process relating classes of systems in presented.…

  10. Data-driven urban drainage analysis: An alternative to hydrodynamic models?

    NARCIS (Netherlands)

    ten Veldhuis, J.A.E.; Tait, S.J.

    2011-01-01

    In the past, there has been an emphasis on the use of hydrodynamic models as a tool for urban drainage analysis. Limited availability of monitoring data and the perceived more limited resource requirements of models led to a preference for this approach. The last decade has seen a gradual developmen

  11. NIRS as an alternative to conventional soil analysis for Greenland soils (focus on SOC)

    DEFF Research Database (Denmark)

    Knadel, Maria; Ogric, Mateja; Adhikari, Kabindra;

    Soil organic carbon (SOC) is an important soil property. It is the main constituents of soil organic matter and a good indicator of soil quality. The estimation and mapping of SOC content could be used to select potential agricultural areas in the Arctic areas. However, conventional analysis of SOC...

  12. Development of systems of analysis in industrial processes with XRF. A technology transfer alternative

    International Nuclear Information System (INIS)

    The FRX Laboratory's experience in developing a unit of analysis by excitation with a radioisotope source is described, with a discussion of its advantages, limitations, types of existing units in the market, use, values and state-of-the-art. The evolution of mining and metallurgical processes has led to the development of new technologies that provide quick and precise control of control and analysis operations, avoiding loss of raw material, chemical reagents, waste of materials and time, to obtain a better quality and purer product. The system developed by the FRX Laboratory is relatively low cost compared to other equipment that is available in the market, and it focuses on single element analysis in hydrometallurgical processes. This system uses a NaI (T1) detector with a beryllium window, related electronics, monitor and printer, which controls the operation automatically using an adequate program for taking samples, measuring, analysis, printing results, changing samples, etc. The sampling is continuous, so it does not have to be taken or prepared chemically. This system can extrapolate to other more complex ones, using new kinds of detectors with higher resolution, more modern electronics, and new multichannel cards. The development of this kind of equipment in Chile means that dependence on foreign technology can be avoided by replacing expensive imported equipment, creating our own technology and transferring it to the domestic market, and even generating income by exporting these units and opening new development prospects (au)

  13. Appraisal of Airport Alternatives in Greenland by the use of Risk Analysis and Monte Carlo Simulation

    DEFF Research Database (Denmark)

    Salling, Kim Bang; Leleur, Steen

    2007-01-01

    This paper presents an appraisal study of three different airport proposals in Greenland by the use of an adapted version of the Danish CBA-DK model. The assessment model is based on both a deterministic calculation by the use of conventional cost-benefit analysis and a stochastic calculation...

  14. Michel's Transport Medium as an Alternative to Liquid Nitrogen for PCR Analysis of Skin Biopsy Specimens

    Directory of Open Access Journals (Sweden)

    Logeina Boraiy

    2014-10-01

    Full Text Available Formalin fixation and paraffin embedding are standard procedures for histopathological diagnosis and allow long-term archiving of tissue specimens. The cross-linking properties of formalin cause fragmentation of nucleic acids and reduce the sensitivity of PCR analysis. Michel's medium is a well-established transport medium used by dermatologists for biopsy transport to maintain tissue-fixed immunoreactants prior to direct immunofluorescence and immunoelectron microscopy. Here we report that Michel's medium also allows short-term preservation of DNA for PCR analysis and permits amplification of amplicons larger than 1 kb. Therefore, Michel's medium appears to be a reserve medium for performing PCR when no other samples are available. © 2014 S. Karger AG, Basel

  15. STEREOLOGY AND 3D MICROSCOPY: USEFUL ALTERNATIVES OR COMPETITORS IN THE QUANTITATIVE ANALYSIS OF MICROSTRUCTURES?

    Directory of Open Access Journals (Sweden)

    Hans Eckart Exner

    2011-05-01

    Full Text Available With the rapid development of modern techniques for producing 3D images, the assessment of 3D geometry from 2D sections of projections by stereological methods seems to become more and more redundant. The paper aims to show the limits of the two approaches and to outline their relative advantages in practical applications. It is concluded that, for a large variety of applications, classical stereological methods are the most effective way to characterize 3D geometry of irregular microstructures. The basic equations for useful global (field parameters are summarized and their assessment by manual techniques is indicated. For other types of applications asking for complex parameters like shape, arrangement or size distribution, preference should be given to direct 3D measurements. Parameters obtained by 2D analysis of sections or projections are useful for comparison purposes, for empirical correlation analysis or for fingerprinting-type description. Field and feature parameters and the problems of data reductions are discussed.

  16. An Empirical Analysis of Television Commercial Ratings in Alternative Competitive Environments Using Multinomial Logit Model

    Directory of Open Access Journals (Sweden)

    Dilek ALTAŞ

    2013-05-01

    Full Text Available Watching the commercials depends on the choice of the viewer. Most of the television viewing takes place during “Prime-Time” unfortunately; many viewers opt to zap to other channels when commercials start. The television viewers’ demographic characteristics may indicate the likelihood of the zapping frequency. Analysis made by using Multinomial Logit Model indicates how effective the demographic variables are in the watching rate of the first minute of the television commercials.

  17. Data-driven urban drainage analysis: An alternative to hydrodynamic models?

    OpenAIRE

    Ten Veldhuis, J.A.E.; Tait, S.J.

    2011-01-01

    In the past, there has been an emphasis on the use of hydrodynamic models as a tool for urban drainage analysis. Limited availability of monitoring data and the perceived more limited resource requirements of models led to a preference for this approach. The last decade has seen a gradual development of water quantity and quality monitoring systems through the development of reliable and increasingly cost-efficient water level sensors, continuous water quality sensors and data communication a...

  18. Michel's Transport Medium as an Alternative to Liquid Nitrogen for PCR Analysis of Skin Biopsy Specimens

    OpenAIRE

    Boraiy, Logeina; Fontao, Lionel

    2014-01-01

    Formalin fixation and paraffin embedding are standard procedures for histopathological diagnosis and allow long-term archiving of tissue specimens. The cross-linking properties of formalin cause fragmentation of nucleic acids and reduce the sensitivity of PCR analysis. Michel's medium is a well-established transport medium used by dermatologists for biopsy transport to maintain tissue-fixed immunoreactants prior to direct immunofluorescence and immunoelectron microscopy. Here we report that M...

  19. Laser assisted ratio analysis - An alternative to GC/IRMS for CO2

    International Nuclear Information System (INIS)

    A new technique for laser based analysis of carbon isotope ratios, with the acronym LARA, based on large isotope shifts in molecular spectra, the use of fixed frequency isotopic lasers, and sensitive detection via the laser optogalvanic effect is reviewed and compared with GC/IRMS for carbon dioxide in specific applications. The possibility for development of new classes of isotope ratio measurement systems with LARA is explored. (author)

  20. Techno-economic analysis of fuel cell auxiliary power units as alternative to idling

    Science.gov (United States)

    Jain, Semant; Chen, Hsieh-Yeh; Schwank, Johannes

    This paper presents a techno-economic analysis of fuel-cell-based auxiliary power units (APUs), with emphasis on applications in the trucking industry and the military. The APU system is intended to reduce the need for discretionary idling of diesel engines or gas turbines. The analysis considers the options for on-board fuel processing of diesel and compares the two leading fuel cell contenders for automotive APU applications: proton exchange membrane fuel cell and solid oxide fuel cell. As options for on-board diesel reforming, partial oxidation and auto-thermal reforming are considered. Finally, using estimated and projected efficiency data, fuel consumption patterns, capital investment, and operating costs of fuel-cell APUs, an economic evaluation of diesel-based APUs is presented, with emphasis on break-even periods as a function of fuel cost, investment cost, idling time, and idling efficiency. The analysis shows that within the range of parameters studied, there are many conditions where deployment of an SOFC-based APU is economically viable. Our analysis indicates that at an APU system cost of 100 kW -1, the economic break-even period is within 1 year for almost the entire range of conditions. At 500 kW -1 investment cost, a 2-year break-even period is possible except for the lowest end of the fuel consumption range considered. However, if the APU investment cost is 3000 kW -1, break-even would only be possible at the highest fuel consumption scenarios. For Abram tanks, even at typical land delivered fuel costs, a 2-year break-even period is possible for APU investment costs as high as 1100 kW -1.

  1. Advancing Risk Analysis for Nanoscale Materials: Report from an International Workshop on the Role of Alternative Testing Strategies for Advancement.

    Science.gov (United States)

    Shatkin, J A; Ong, Kimberly J; Beaudrie, Christian; Clippinger, Amy J; Hendren, Christine Ogilvie; Haber, Lynne T; Hill, Myriam; Holden, Patricia; Kennedy, Alan J; Kim, Baram; MacDonell, Margaret; Powers, Christina M; Sharma, Monita; Sheremeta, Lorraine; Stone, Vicki; Sultan, Yasir; Turley, Audrey; White, Ronald H

    2016-08-01

    The Society for Risk Analysis (SRA) has a history of bringing thought leadership to topics of emerging risk. In September 2014, the SRA Emerging Nanoscale Materials Specialty Group convened an international workshop to examine the use of alternative testing strategies (ATS) for manufactured nanomaterials (NM) from a risk analysis perspective. Experts in NM environmental health and safety, human health, ecotoxicology, regulatory compliance, risk analysis, and ATS evaluated and discussed the state of the science for in vitro and other alternatives to traditional toxicology testing for NM. Based on this review, experts recommended immediate and near-term actions that would advance ATS use in NM risk assessment. Three focal areas-human health, ecological health, and exposure considerations-shaped deliberations about information needs, priorities, and the next steps required to increase confidence in and use of ATS in NM risk assessment. The deliberations revealed that ATS are now being used for screening, and that, in the near term, ATS could be developed for use in read-across or categorization decision making within certain regulatory frameworks. Participants recognized that leadership is required from within the scientific community to address basic challenges, including standardizing materials, protocols, techniques and reporting, and designing experiments relevant to real-world conditions, as well as coordination and sharing of large-scale collaborations and data. Experts agreed that it will be critical to include experimental parameters that can support the development of adverse outcome pathways. Numerous other insightful ideas for investment in ATS emerged throughout the discussions and are further highlighted in this article.

  2. Advancing Risk Analysis for Nanoscale Materials: Report from an International Workshop on the Role of Alternative Testing Strategies for Advancement.

    Science.gov (United States)

    Shatkin, J A; Ong, Kimberly J; Beaudrie, Christian; Clippinger, Amy J; Hendren, Christine Ogilvie; Haber, Lynne T; Hill, Myriam; Holden, Patricia; Kennedy, Alan J; Kim, Baram; MacDonell, Margaret; Powers, Christina M; Sharma, Monita; Sheremeta, Lorraine; Stone, Vicki; Sultan, Yasir; Turley, Audrey; White, Ronald H

    2016-08-01

    The Society for Risk Analysis (SRA) has a history of bringing thought leadership to topics of emerging risk. In September 2014, the SRA Emerging Nanoscale Materials Specialty Group convened an international workshop to examine the use of alternative testing strategies (ATS) for manufactured nanomaterials (NM) from a risk analysis perspective. Experts in NM environmental health and safety, human health, ecotoxicology, regulatory compliance, risk analysis, and ATS evaluated and discussed the state of the science for in vitro and other alternatives to traditional toxicology testing for NM. Based on this review, experts recommended immediate and near-term actions that would advance ATS use in NM risk assessment. Three focal areas-human health, ecological health, and exposure considerations-shaped deliberations about information needs, priorities, and the next steps required to increase confidence in and use of ATS in NM risk assessment. The deliberations revealed that ATS are now being used for screening, and that, in the near term, ATS could be developed for use in read-across or categorization decision making within certain regulatory frameworks. Participants recognized that leadership is required from within the scientific community to address basic challenges, including standardizing materials, protocols, techniques and reporting, and designing experiments relevant to real-world conditions, as well as coordination and sharing of large-scale collaborations and data. Experts agreed that it will be critical to include experimental parameters that can support the development of adverse outcome pathways. Numerous other insightful ideas for investment in ATS emerged throughout the discussions and are further highlighted in this article. PMID:27510619

  3. Techno-Economic Analysis of Biogas Utilization as an Alternative Fuel

    Directory of Open Access Journals (Sweden)

    Merry Indahsari Devi

    2014-07-01

    Full Text Available This paper will discuss the feasibility and economic analysis of biogas energy as a supply for the diesel engine generator. The techno-economic analysis was performed by using three parameters which are Net Present Value (NPV, Internal Rate of Return (IRR, and Payback Period (PP as the feasibility indicators of the biogas power plant project. Calculation of substitution was obtained from the comparison between data of diesel engine using diesel fuel and dual-fuel with biogas. Economic calculations include the substitution percentage of diesel fuel by biogas for dual-fuel. Meanwhile, the calculation of savings was based on the ratio of energy content between diesel fuel and biogas. The eventual outcome is determined using economic comparison between the use of diesel fuel and dual-fuel mode. Feasibility shows that the pilot plant of 1 to 6 kWh using diesel fuel and dual-fuel are not feasible while techno-economic parameter analysis shows that NPV<0, IRR

  4. Expression analysis of an evolutionarily conserved alternative splicing factor, Sfrs10, in age-related macular degeneration.

    Directory of Open Access Journals (Sweden)

    Devi Krishna Priya Karunakaran

    Full Text Available Age-related macular degeneration (AMD is the most common cause of blindness in the elderly population. Hypoxic stress created in the micro-environment of the photoreceptors is thought to be the underlying cause that results in the pathophysiology of AMD. However, association of AMD with alternative splicing mediated gene regulation is not well explored. Alternative Splicing is one of the primary mechanisms in humans by which fewer protein coding genes are able to generate a vast proteome. Here, we investigated the expression of a known stress response gene and an alternative splicing factor called Serine-Arginine rich splicing factor 10 (Sfrs10. Sfrs10 is a member of the serine-arginine (SR rich protein family and is 100% identical at the amino acid level in most mammals. Immunoblot analysis on retinal extracts from mouse, rat, and chicken showed a single immunoreactive band. Further, immunohistochemistry on adult mouse, rat and chicken retinae showed pan-retinal expression. However, SFRS10 was not detected in normal human retina but was observed as distinct nuclear speckles in AMD retinae. This is in agreement with previous reports that show Sfrs10 to be a stress response gene, which is upregulated under hypoxia. The difference in the expression of Sfrs10 between humans and lower mammals and the upregulation of SFRS10 in AMD is further reflected in the divergence of the promoter sequence between these species. Finally, SFRS10+ speckles were independent of the SC35+ SR protein speckles or the HSF1+ stress granules. In all, our data suggests that SFRS10 is upregulated and forms distinct stress-induced speckles and might be involved in AS of stress response genes in AMD.

  5. Alternating current impedance spectroscopic analysis of biofunctionalized vertically-aligned silica nanospring surface for biosensor applications

    Science.gov (United States)

    Timalsina, Yukta P.

    In this dissertation, a process of vertically-aligned (silica) nanosprings (VANS) based biosensor development is presented. Alternating current (AC) impedance spectroscopy has been used to analyze sensor response as a function of saline phosphate (SP) buffer and biological solutions. The sensor is a parallel plate capacitor consisting of two glass substrates coated with indium tin oxide (ITO), where the VANS [or randomly-aligned nanosprings (RANS)] grown on one substrate serve as the dielectric spacer layer. The response of a VANS device as a function of ionic concentration in SP buffer was examined and an equivalent circuit model was developed. The results demonstrated that VANS sensors exhibited greater sensitivity to the changes in SP concentration relative to the ITO sensors, which serve as controls. The biofunctionalized VANS surface via physisorption and the cross-linker method demonstrates the repeatability, specificity, and selectivity of the binding. The physisorption of biotinylated immunoglobulin G (B-IgG) onto the VANS surface simplifies the whole sensing procedure for the detection of glucose oxidase, since the avidin-conjugated glucose oxidase (Av-GOx) can directly be immobilized on the B-IgG. The cross linker method involves the covalent attachment of antibodies onto the functionalized VANS surface via imine bond. The experiments revealed that the VANS sensor response is solely the result of the interaction of target molecule i.e. mouse IgG with the probe layer, i.e. goat antimouse IgG (GalphaM IgG). It was determined that VANS-based sensors exhibit a greater magnitude of change between successive bio-layers relative to the controls above 100 Hz, which indicates that the addition of biomolecules inhibits the diffusion of ions and changes the effective dielectric response of the VANS via biomolecular polarization. The study of ionic transport in nanosprings suggested that conductance follows a scaling law. It was demonstrated that a VANS-based device

  6. Rapid architecture alternative modeling (RAAM): A framework for capability-based analysis of system of systems architectures

    Science.gov (United States)

    Iacobucci, Joseph V.

    The research objective for this manuscript is to develop a Rapid Architecture Alternative Modeling (RAAM) methodology to enable traceable Pre-Milestone A decision making during the conceptual phase of design of a system of systems. Rather than following current trends that place an emphasis on adding more analysis which tends to increase the complexity of the decision making problem, RAAM improves on current methods by reducing both runtime and model creation complexity. RAAM draws upon principles from computer science, system architecting, and domain specific languages to enable the automatic generation and evaluation of architecture alternatives. For example, both mission dependent and mission independent metrics are considered. Mission dependent metrics are determined by the performance of systems accomplishing a task, such as Probability of Success. In contrast, mission independent metrics, such as acquisition cost, are solely determined and influenced by the other systems in the portfolio. RAAM also leverages advances in parallel computing to significantly reduce runtime by defining executable models that are readily amendable to parallelization. This allows the use of cloud computing infrastructures such as Amazon's Elastic Compute Cloud and the PASTEC cluster operated by the Georgia Institute of Technology Research Institute (GTRI). Also, the amount of data that can be generated when fully exploring the design space can quickly exceed the typical capacity of computational resources at the analyst's disposal. To counter this, specific algorithms and techniques are employed. Streaming algorithms and recursive architecture alternative evaluation algorithms are used that reduce computer memory requirements. Lastly, a domain specific language is created to provide a reduction in the computational time of executing the system of systems models. A domain specific language is a small, usually declarative language that offers expressive power focused on a particular

  7. A Cost Benefit Analysis of Two Alternative Traffic Lights Systems for the Colourful City of Zamzam

    OpenAIRE

    Machimbirike, Alexious

    2012-01-01

    ABSTRACT: Zamzam Infrastructure Bank has been approached by the country’s third largest city to finance the upgrading and expansion of its traffic lights system. Since the project is social in nature, the Bank intends to justify the borrowing on the basis of Colourful City’s ability to service the loan from its consolidated cash flows. A pre-requisite however, is the need to confirm the project’s socio-economic viability through economic cost benefit analysis. The Bank assessed and is satis...

  8. Analysis of microstrip lines with alternative implementation of conductors and superconductors

    Science.gov (United States)

    Kong, K.-S.; Lee, H.-Y.; Itoh, T.; Chorey, C. M.; Bhasin, K. B.

    1990-01-01

    An analysis of microstrip line structures in which either the strip or the ground plane or both are made of a high Tc superconductor is presented. The effect of implementation of a superconductor to the strip and the ground plane is explained with the calculation of a conductor loss of the structure by the Phenomenological Loss Equivalence Method (PEM). The theoretical values are compared with the experimental results from a ring resonator which is made of a gold ground plane and a high Tc superconductor, YBa2Cu3O(7-x), strip.

  9. Analysis of cost and accuracy of alternative strategies for Enterobacteriaceae identification.

    Science.gov (United States)

    Robertson, E A; Macks, G C; MacLowry, J D

    1976-04-01

    Analysis of the cost of time and material required for the diagnosis of Enterobacteriacea isolates indicated that a conventional 17-tube (20-test) setup costs $7.98 per isolated identified. Using the API 20E, a similar identification cost $3.02. A conventional 7-tube (10-test) setup cost $3.60, whereas the comparable cost by 30% while increasing the number of isolate identified correctly by 3%. Other strategres using the API 20E or a deoxyribounclease test were also evaluated for cost and accuracy. PMID:770498

  10. Evaluation of alternative pollutant emission control strategies to urban water systems using substance flow analysis

    DEFF Research Database (Denmark)

    Lundy, L.; Revitt, D. M.; Eriksson, Eva;

    2011-01-01

    results from the ScorePP project (an EU Framework Programme 6-funded consortium) which developed and assessed the performance of a series of emission control strategies (ECS) at an urban scale. Data from the literature, in combination with expert judgement (where data was not available), have been used...... to develop semi-hypothetical case cities (SHCCs), which represent virtual platforms for the evaluation of ECSs using substance flow analysis. The results indicate that the full implementation of existing legislation is capable of reducing total emissions of Cd and Hg by between 11% and 20%. Consideration...

  11. Assessment of alternate procedures for the seismic analysis of multiply supported piping systems

    International Nuclear Information System (INIS)

    When response spectrum methods are used in the seismic analysis of piping systems the response due to inertial action, the dynamic response, and the response due to the time varying differential motions of the support points (the pseudo-static response) must be determined. In this study the adequacy and the degree of conservatism associated with the uniform response spectrum method, the center of mass response spectrum method and fourteen variants of the independent response spectrum method to compute the dynamic response and five different methods to compute the pseudo-static response were evaluated. For this purpose a sample of six piping systems, two of which were subjected to thirty-three earthquakes, were studied. For each system and seismic excitation a multiple independent support excitation time history analysis was developed and used to provide a best estimate of true response and to form the basis for comparison. A combination procedure to calculate the total responses is considered as well. Results are presented and compared to the corresponding responses evaluated using the current uniform response spectrum method and the center of mass response spectra approach. Based on the results, recommendations concerning the use of the methods were developed

  12. Socioeconomic effects of power marketing alternatives for the Central Valley and Washoe Projects: 2005 regional econmic impact analysis using IMPLAN

    International Nuclear Information System (INIS)

    The Western Area Power Administration (Western) was founded by the Department of Energy Organization Act of 1977 to market and transmit federal hydroelectric power in 15 western states outside the Pacific Northwest, which is served by the Bonneville Power Administration. Western is divided into four independent Customer Service Regions including the Sierra Nevada Region (Sierra Nevada), the focus of this report. The Central Valley Project (CVP) and the Washoe Project provide the primary power resources marketed by Sierra Nevada. Sierra Nevada also purchases and markets power generated by the Bonneville Power Administration, Pacific Gas and Electric (PG ampersand E), and various power pools. Sierra Nevada currently markets approximately 1,480 megawatts of power to 77 customers in northern and central California. These customers include investor-owned utilities, public utilities, government agencies, military bases, and irrigation districts. Methods and conclusions from an economic analysis are summarized concerning distributional effects of alternative actions that Sierra Nevada could take with it's new marketing plan

  13. Finite Element Analysis of Synergy Effect on Concrete Beams Incorporated with Coated Reinforcement and Alternate Aggregates

    Directory of Open Access Journals (Sweden)

    Sakthivel Pandiaraj

    2016-01-01

    Full Text Available The purpose of this study is to compare the ultimate load carrying capacity of conventional reinforced concrete beams with that of investigation specimen incorporated with coated reinforcement and partially with recycled aggregate and quarry dust. A novel technique of coated reinforcement delays the onset of corrosion with enhanced durability of structures. Results show that not even a film of corrosion (white rust can be seen in the investigation specimen. There is a progressive increase in stiffness from the state of the first crack to ultimate stage and a negligible difference in ultimate load carrying capacity of the investigation specimen, when compared with the controlled specimen. Incorporation of galvanization, recycled aggregate, and quarry dust seemed to be compatible with the existing conservative concreting procedures. Experimental results are compared with the numerical solutions aided by finite element analysis (FEA by using ABAQUS.

  14. Employment relations, social class and health: a review and analysis of conceptual and measurement alternatives.

    Science.gov (United States)

    Muntaner, Carles; Borrell, Carme; Vanroelen, Christophe; Chung, Haejoo; Benach, Joan; Kim, Il Ho; Ng, Edwin

    2010-12-01

    Employment relations, as a theoretical framework for social class, represent a complementary approach to social stratification. Employment relations introduce social relations of ownership and control over productive assets to the analysis of inequalities in economic (e.g., income), power (occupational hierarchy), and cultural (e.g., education) resources. The objectives of this paper are to briefly clarify the theoretical background on socio-economic indicators used in social epidemiology and to conduct a review of empirical studies that adopt relational social class indicators in the socio-epidemiological literature. Measures of employment relations in social determinants of health research can be classified within two major conceptual frameworks: 1) "Neo-Weberian", like the National Statistics Socio-Economic Classification (NS-SEC) which is widely used in the United Kingdom; and 2) "Neo-Marxian", like Erik O. Wright's social class indicators, which are being used by social epidemiologists in the Americas and Europe. Our review of empirical findings (49 articles found) reveals that the relation between employment relations and health does not necessarily imply a graded relationship. For example, small employers can exhibit worse health than highly skilled workers, and supervisors can display worse health than frontline workers. The policy implications of employment relations research are therefore different, and complement those of income or education health gradient studies. While the latter studies tend to emphasize income redistribution policy options, employment relations implicate other factors such as workplace democracy and social protection. Our analysis confirms that the current transformation of employment relations calls for new social class concepts and measures to explain social inequalities in health and to generate policies to reduce them.

  15. Weighted Kolmogorov Smirnov testing: an alternative for Gene Set Enrichment Analysis.

    Science.gov (United States)

    Charmpi, Konstantina; Ycart, Bernard

    2015-06-01

    Gene Set Enrichment Analysis (GSEA) is a basic tool for genomic data treatment. Its test statistic is based on a cumulated weight function, and its distribution under the null hypothesis is evaluated by Monte-Carlo simulation. Here, it is proposed to subtract to the cumulated weight function its asymptotic expectation, then scale it. Under the null hypothesis, the convergence in distribution of the new test statistic is proved, using the theory of empirical processes. The limiting distribution needs to be computed only once, and can then be used for many different gene sets. This results in large savings in computing time. The test defined in this way has been called Weighted Kolmogorov Smirnov (WKS) test. Using expression data from the GEO repository, tested against the MSig Database C2, a comparison between the classical GSEA test and the new procedure has been conducted. Our conclusion is that, beyond its mathematical and algorithmic advantages, the WKS test could be more informative in many cases, than the classical GSEA test.

  16. An alternative approach for addressing the failure probability-safety factor method with sensitivity analysis

    Energy Technology Data Exchange (ETDEWEB)

    Castillo, Enrique; Conejo, Antonio J.; Minguez, Roberto; Castillo, Carmen

    2003-11-01

    The paper introduces a method for solving the failure probability-safety factor problem for designing engineering works proposed by Castillo et al. that optimizes an objective function subject to the standard geometric and code constraints, and two more sets of constraints that simultaneously guarantee given safety factors and failure probability bounds associated with a given set of failure modes. The method uses the dual variables and is especially convenient to perform a sensitivity analysis, because sensitivities of the objective function and the reliability indices can be obtained with respect to all data values. To this end, the optimization problems are transformed into other equivalent ones, in which the data parameters are converted into artificial variables, and locked to their actual values. In this way, some variables of the associated dual problems become the desired sensitivities. In addition, using the proposed methodology, calibration of codes based on partial safety factors can be done. The method is illustrated by its application to the design of a simple rubble mound breakwater and a bridge crane.

  17. Alternative industrial carbon emissions benchmark based on input-output analysis

    Science.gov (United States)

    Han, Mengyao; Ji, Xi

    2016-05-01

    Some problems exist in the current carbon emissions benchmark setting systems. The primary consideration for industrial carbon emissions standards highly relate to direct carbon emissions (power-related emissions) and only a portion of indirect emissions are considered in the current carbon emissions accounting processes. This practice is insufficient and may cause double counting to some extent due to mixed emission sources. To better integrate and quantify direct and indirect carbon emissions, an embodied industrial carbon emissions benchmark setting method is proposed to guide the establishment of carbon emissions benchmarks based on input-output analysis. This method attempts to link direct carbon emissions with inter-industrial economic exchanges and systematically quantifies carbon emissions embodied in total product delivery chains. The purpose of this study is to design a practical new set of embodied intensity-based benchmarks for both direct and indirect carbon emissions. Beijing, at the first level of carbon emissions trading pilot schemes in China, plays a significant role in the establishment of these schemes and is chosen as an example in this study. The newly proposed method tends to relate emissions directly to each responsibility in a practical way through the measurement of complex production and supply chains and reduce carbon emissions from their original sources. This method is expected to be developed under uncertain internal and external contexts and is further expected to be generalized to guide the establishment of industrial benchmarks for carbon emissions trading schemes in China and other countries.

  18. Refinement of Stereo Image Analysis Using Photometric Shape Recovery as AN Alternative to Bundle Adjustment

    Science.gov (United States)

    Grumpe, A.; Schröer, C.; Kauffmann, S.; Fricke, T.; Wöhler, C.; Mall, U.

    2016-06-01

    Topographic mapping, e.g. the generation of Digital Elevation Models (DEM), is of general interest to the remote sensing community and scientific research. Commonly, photogrammetric methods, e.g. stereo image analysis methods (SIAM) or bundle adjustment methods (BAM), are applied to derive 3D information based on multiple images of an area. These methods require the detection of control points, i.e. common points within multiple images, which relies on a similarity measure and usually yields a sparse map of 3D points. The full spatial DEM is then obtained by interpolation techniques or imposed restrictions, e.g. smoothness constraints. Since BAM utilizes all images of the area, it is assumed to provide a more accurate DEM than SIAM which utilizes only pairs of images. Intensity-based shape recovery, e.g. shape from shading (SfS), utilizes the reflectance behavior of the object surface and thus provides a dense map of relative height changes, which provide the possibility to refine the photogrammetric DEMs. Based on Rosetta NavCam images of 67P/Churyumov-Gerasimenko we compare intensity-based DEM refinement methods which use DEMs obtained based on SIAM and BAM as a reference. We show that both the SIAM based DEM refinement and the BAM based DEM refinement are of similar quality. It is thus possible to derive DEMs of high lateral resolution by applying the intensity-based refinement to the less complex SIAM.

  19. Individual organisms as units of analysis: Bayesian-clustering alternatives in population genetics.

    Science.gov (United States)

    Mank, Judith E; Avise, John C

    2004-12-01

    Population genetic analyses traditionally focus on the frequencies of alleles or genotypes in 'populations' that are delimited a priori. However, there are potential drawbacks of amalgamating genetic data into such composite attributes of assemblages of specimens: genetic information on individual specimens is lost or submerged as an inherent part of the analysis. A potential also exists for circular reasoning when a population's initial identification and subsequent genetic characterization are coupled. In principle, these problems are circumvented by some newer methods of population identification and individual assignment based on statistical clustering of specimen genotypes. Here we evaluate a recent method in this genre--Bayesian clustering--using four genotypic data sets involving different types of molecular markers in non-model organisms from nature. As expected, measures of population genetic structure (F(ST) and phiST) tended to be significantly greater in Bayesian a posteriori data treatments than in analyses where populations were delimited a priori. In the four biological contexts examined, which involved both geographic population structures and hybrid zones, Bayesian clustering was able to recover differentiated populations, and Bayesian assignments were able to identify likely population sources of specific individuals.

  20. System analysis and assessment of technological alternatives for Nordic H{sub 2} energy foresight

    Energy Technology Data Exchange (ETDEWEB)

    Koljonen, T.; Pursiheimo, E. [VTT, Espoo (Finland); Gether, K. [NTNU, Trondheim (Norway); Joergensen, K. [Risoe National Lab. (Denmark)

    2004-12-01

    The hydrogen scenarios developed during the Nordic Hydrogen Foresight project was analysed using a energy system model, which was developed during the project. The aim of the systems analysis was to analyse the technical and economical potential of hydrogen society in the Nordic countries in quantitative terms as well as the competitiveness of the selected hydrogen based systems. Visions and scenarios of the future energy systems in the Nordic area were defined in the workshops of the project. As a result of these workshops three scenarios were selected to outline the future of Nordic energy. The scenarios included different energy policies; scenarios for fossil fuel prices; and hydrogen energy demands, which varied from 6% to 18% of the total energy demand in 2030 for transport sector, and from 3% to 9% in heat and power production. In the roadmap workshops, the most important hydrogen based systems were selected, which were also included in the model. These include steam reforming of natural gas, electrolysis with renewable electricity, and biomass gasification for hydrogen production. For stationary applications, fuel cells and gas engines were selected for power and heat production. In our scenario calculations, biomass gasification and steam reforming seem to be the most competitive technologies for hydrogen production. The competitiveness of biomass gasification is greatly affected by the biomass fuel price, which is a local energy source. Electrolysis seems to be most competitive in decentralized systems, if the electricity price is low enough. For stationary applications, CHP fuel cells seem to be the most competitive in the long term, if the technological development and the decrease in investment costs follow the assumed scenario. The approximated Nordic market sizes in 2030 for the base scenarios varied from 1000 ME to 3000 MEuro for hydrogen production, from 1000 to 4000 MEuro for stationary applications and 4000 MEuro to 12.000 MEuro for hydrogen

  1. Multiplex gas chromatography: an alternative concept for gas chromatographic analysis of planetary atmospheres

    Science.gov (United States)

    Valentin, J. R.

    1989-01-01

    -Cassini entry probe, which is being jointly planned by NASA and the European Space Agency (ESA), might be launched as early as 1994. As in the Pioneer mission, limited time--perhaps only 3-4 h--will be available for the completion of all analyses while the probe descends through the atmosphere. A conventional GC or GC-MS system would be able to analyze no more than two aerosol and two gas samples during the probe's descent. Conventional GC also is limited by the sensitivity of the detector and by the sample volume. For the Titan mission, the sensitivity problems will be worse because the atmospheric pressure at the time of instrument deployment is expected to be chromatography has been investigated as a possible candidate for chemical analysis within a spacecraft or other restricted environment, and chemical modulators have been developed and used when needed with this technique to reduce the size and weight of the instrumentation. Also, several new multiplex techniques have been developed for use in specific applications.

  2. Allelic variation, alternative splicing and expression analysis of Psy1 gene in Hordeum chilense Roem. et Schult.

    Directory of Open Access Journals (Sweden)

    Cristina Rodríguez-Suárez

    Full Text Available BACKGROUND: The wild barley Hordeum chilense Roem. et Schult. is a valuable source of genes for increasing carotenoid content in wheat. Tritordeums, the amphiploids derived from durum or common wheat and H. chilense, systematically show higher values of yellow pigment colour and carotenoid content than durum wheat. Phytoene synthase 1 gene (Psy1 is considered a key step limiting the carotenoid biosynthesis, and the correlation of Psy1 transcripts accumulation and endosperm carotenoid content has been demonstrated in the main grass species. METHODOLOGY/PRINCIPAL FINDINGS: We analyze the variability of Psy1 alleles in three lines of H. chilense (H1, H7 and H16 representing the three ecotypes described in this species. Moreover, we analyze Psy1 expression in leaves and in two seed developing stages of H1 and H7, showing mRNA accumulation patterns similar to those of wheat. Finally, we identify thirty-six different transcripts forms originated by alternative splicing of the 5' UTR and/or exons 1 to 5 of Psy1 gene. Transcripts function is tested in a heterologous complementation assay, revealing that from the sixteen different predicted proteins only four types (those of 432, 370, 364 and 271 amino acids, are functional in the bacterial system. CONCLUSIONS/SIGNIFICANCE: The large number of transcripts originated by alternative splicing of Psy1, and the coexistence of functional and non functional forms, suggest a fine regulation of PSY activity in H. chilense. This work is the first analysis of H. chilense Psy1 gene and the results reported here are the bases for its potential use in carotenoid enhancement in durum wheat.

  3. Battlefield Utility of Antipersonnel Landmines and Proposed Alternatives (Analysis in Support of the NATO SAS-023 APM Study)

    Energy Technology Data Exchange (ETDEWEB)

    Crandley, J F; Greenwalt, R J; Magnoli, D E; Randazzo, A S

    2002-02-05

    This study consists of work done in support of the U.S. delegation to the NATO SAS-023 Antipersonnel Landmine Study Group, supplemented by additional work done for the U.S. Office of the Secretary of Defense Antipersonnel Landmine Alternative Concept Exploration Program (Track III). It explores the battlefield utility of current antipersonnel landmines (APL) in both pure and mixed APL/antitank minefields and evaluates the value of military suggested non-materiel alternatives. The historical record is full of examples where the presence (or absence) of antipersonnel landmines made a critical difference in battle. The current generation of military thinkers and writers lack any significant combat experience employing either mixed or antipersonnel minefields, which leaves a critical gap in available expert advice for policy and decision-makers. Because of this lack of experienced-based professional military knowledge, Lawrence Livermore National Laboratory analyzed the employment of antipersonnel landmines in tactical mixed minefields and in protective antipersonnel minefields. The scientific method was employed where hypotheses were generated from the tactics and doctrine of the antipersonnel landmine era and tested in a simulation laboratory. A high-resolution, U.S. Joint Forces Command combat simulation model (the Joint Conflict and Tactical Simulation--JCATS) was used as the laboratory instrument. A realistic European scenario was obtained from a multi-national USAREUR exercise and was approved by the SAS-023 panel members. Additional scenarios were provided by U.S. CINC conferences and were based on Southwest Asia and Northeast Asia. Weapons data was obtained from the U.S. family of Joint Munitions Effectiveness Manuals. The U.S. Army Materiel Systems Analysis Agency conducted a limited verification and validation assessment of JCATS for purposes of this study.

  4. Comparative Analysis of Alternative Spectral Bands of CO2 and O2 for the Sensing of CO2 Mixing Ratios

    Science.gov (United States)

    Pliutau, Denis; Prasad, Narasimha S.

    2013-01-01

    We performed comparative studies to establish favorable spectral regions and measurement wavelength combinations in alternative bands of CO2 and O2, for the sensing of CO2 mixing ratios (XCO2) in missions such as ASCENDS. The analysis employed several simulation approaches including separate layers calculations based on pre-analyzed atmospheric data from the modern-era retrospective analysis for research and applications (MERRA), and the line-byline radiative transfer model (LBLRTM) to obtain achievable accuracy estimates as a function of altitude and for the total path over an annual span of variations in atmospheric parameters. Separate layer error estimates also allowed investigation of the uncertainties in the weighting functions at varying altitudes and atmospheric conditions. The parameters influencing the measurement accuracy were analyzed independently and included temperature sensitivity, water vapor interferences, selection of favorable weighting functions, excitations wavelength stabilities and other factors. The results were used to identify favorable spectral regions and combinations of on / off line wavelengths leading to reductions in interferences and the improved total accuracy.

  5. Molecular Analysis of an Alternative N-Glycosylation Machinery by Functional Transfer from Actinobacillus pleuropneumoniae to Escherichia coli*

    Science.gov (United States)

    Naegeli, Andreas; Neupert, Christine; Fan, Yao-Yun; Lin, Chia-Wei; Poljak, Kristina; Papini, Anna Maria; Schwarz, Flavio; Aebi, Markus

    2014-01-01

    N-Linked protein glycosylation is a frequent post-translational modification that can be found in all three domains of life. In a canonical, highly conserved pathway, an oligosaccharide is transferred by a membrane-bound oligosaccharyltransferase from a lipid donor to asparagines in the sequon NX(S/T) of secreted polypeptides. The δ-proteobacterium Actinobacillus pleuropneumoniae encodes an unusual pathway for N-linked protein glycosylation. This pathway takes place in the cytoplasm and is mediated by a soluble N-glycosyltransferase (NGT) that uses nucleotide-activated monosaccharides to glycosylate asparagine residues. To characterize the process of cytoplasmic N-glycosylation in more detail, we studied the glycosylation in A. pleuropneumoniae and functionally transferred the glycosylation system to Escherichia coli. N-Linked glucose specific human sera were used for the analysis of the glycosylation process. We identified autotransporter adhesins as the preferred protein substrate of NGT in vivo, and in depth analysis of the modified sites in E. coli revealed a surprisingly relaxed peptide substrate specificity. Although NX(S/T) is the preferred acceptor sequon, we detected glycosylation of alternative sequons, including modification of glutamine and serine residues. We also demonstrate the use of NGT to glycosylate heterologous proteins. Therefore, our study could provide the basis for a novel route for the engineering of N-glycoproteins in bacteria. PMID:24275653

  6. Evaluation Outcomes Associated with Alternative Dosing Strategies for Piperacillin/Tazobactam: A Systematic Review and Meta-Analysis.

    Science.gov (United States)

    Yang, Hui; Cui, Xiangli; Ma, Zhuo; Liu, Lihong

    2016-01-01

    A better dosing strategy can improve clinical outcomes for patients. We systematically reviewed the literatures to determine whether any clinical benefits exist for piperacillin/tazobactam by extended or continuous infusion. Methods - A search of PubMed, Web of Science, ProQuest, ScienceDirect, Cochrane, Embase and related ICAAC and ACCP conferences were conducted up to September 5, 2015. Randomized controlled and observational studies that compared extended or continuous infusion with conventional intermittent infusion of piperacillin/tazobactam were identified from the databases above and analyzed. Two reviewers independently evaluated the methodology and extracted data from primary studies. A meta-analysis was performed using Revman 5.2 software. The quality of each study was assessed. Sensitivity analysis and publication bias were evaluated. Results - Three randomized controlled trials and twelve observational studies were included in this study. All included studies had high quality and no publication bias was found. Compared to the conventional intermittent infusion approach, the extended or continuous infusion group had a significant cost effectiveness (OR -0.89.02, CI (-114.69,-63.35), P<0.00001). No statistical difference was observed for clinical cure rate (OR 1.64, 95% CI (0.88, 3.30), P=0.12) between the two dosing regimens. The sensitivity analysis showed the results were stable. Conclusions - Our systematic review and meta-analysis found that the outcomes associated with alternative dosing strategies of piperacillin/tazobactam have changed compared with conclusions before for several literatures with large samples published. Further data on the outcomes should be generated for a better understanding of the extended or continuous infusion strategy. On the whole, our meta-analysis suggested that the extended or continuous infusion should be recommended for clinical use only considering its economic advantage, but there was no significantly higher

  7. Advancing Risk Analysis for Nanoscale Materials: Report from an International Workshop on the Role of Alternative Testing Strategies for Advancement: Advancing Risk Analysis for Nanoscale Materials

    Energy Technology Data Exchange (ETDEWEB)

    Shatkin, J. A. [Vireo Advisors, Boston MA USA; Ong, Kimberly J. [Vireo Advisors, Boston MA USA; Beaudrie, Christian [Compass RM, Vancouver CA USA; Clippinger, Amy J. [PETA International Science Consortium Ltd, London UK; Hendren, Christine Ogilvie [Center for the Environmental Implications of NanoTechnology, Duke University, Durham NC USA; Haber, Lynne T. [TERA, Cincinnati OH USA; Hill, Myriam [Health Canada, Ottawa Canada; Holden, Patricia [UC Santa Barbara, Bren School of Environmental Science & Management, ERI, and UC CEIN, University of California, Santa Barbara CA USA; Kennedy, Alan J. [U.S. Army Engineer Research and Development Center, Environmental Laboratory, Vicksburg MS USA; Kim, Baram [Independent, Somerville MA USA; MacDonell, Margaret [Argonne National Laboratory, Environmental Science Division, Argonne IL USA; Powers, Christina M. [U.S. Environmental Protection Agency, Office of Air and Radiation, Office of Transportation and Air Quality, Ann Arbor MI USA; Sharma, Monita [PETA International Science Consortium Ltd, London UK; Sheremeta, Lorraine [Alberta Ingenuity Labs, Edmonton Alberta Canada; Stone, Vicki [John Muir Building Gait 1 Heriot-Watt University, Edinburgh Scotland UK; Sultan, Yasir [Environment Canada, Gatineau QC Canada; Turley, Audrey [ICF International, Durham NC USA; White, Ronald H. [RH White Consultants, Silver Spring MD USA

    2016-08-01

    The Society for Risk Analysis (SRA) has a history of bringing thought leadership to topics of emerging risk. In September 2014, the SRA Emerging Nanoscale Materials Specialty Group convened an international workshop to examine the use of alternative testing strategies (ATS) for manufactured nanomaterials (NM) from a risk analysis perspective. Experts in NM environmental health and safety, human health, ecotoxicology, regulatory compliance, risk analysis, and ATS evaluated and discussed the state of the science for in vitro and other alternatives to traditional toxicology testing for NM. Based on this review, experts recommended immediate and near-term actions that would advance ATS use in NM risk assessment. Three focal areas-human health, ecological health, and exposure considerations-shaped deliberations about information needs, priorities, and the next steps required to increase confidence in and use of ATS in NM risk assessment. The deliberations revealed that ATS are now being used for screening, and that, in the near term, ATS could be developed for use in read-across or categorization decision making within certain regulatory frameworks. Participants recognized that leadership is required from within the scientific community to address basic challenges, including standardizing materials, protocols, techniques and reporting, and designing experiments relevant to real-world conditions, as well as coordination and sharing of large-scale collaborations and data. Experts agreed that it will be critical to include experimental parameters that can support the development of adverse outcome pathways. Numerous other insightful ideas for investment in ATS emerged throughout the discussions and are further highlighted in this article.

  8. Survival analysis in total joint replacement: an alternative method of accounting for the presence of competing risk.

    Science.gov (United States)

    Fennema, P; Lubsen, J

    2010-05-01

    Survival analysis is an important tool for assessing the outcome of total joint replacement. The Kaplan-Meier method is used to estimate the incidence of revision of a prosthesis over time, but does not account appropriately for competing events which preclude revision. In the presence of competing death, this method will lead to statistical bias and the curve will lose its interpretability. A valid comparison of survival results between studies using the method is impossible without accounting for different rates of competing events. An alternative and easily applicable approach, the cumulative incidence of competing risk, is proposed. Using three simulated data sets and realistic data from a cohort of 406 consecutive cementless total hip prostheses, followed up for a minimum of ten years, both approaches were compared and the magnitude of potential bias was highlighted. The Kaplan-Meier method overestimated the incidence of revision by almost 4% (60% relative difference) in the simulations and more than 1% (31.3% relative difference) in the realistic data set. The cumulative incidence of competing risk approach allows for appropriate accounting of competing risk and, as such, offers an improved ability to compare survival results across studies.

  9. Socioeconomic effects of power marketing alternatives for the Central Valley and Washoe Projects: 2005 regional econmic impact analysis using IMPLAN

    Energy Technology Data Exchange (ETDEWEB)

    Anderson, D.M.; Godoy-Kain, P.; Gu, A.Y.; Ulibarri, C.A.

    1996-11-01

    The Western Area Power Administration (Western) was founded by the Department of Energy Organization Act of 1977 to market and transmit federal hydroelectric power in 15 western states outside the Pacific Northwest, which is served by the Bonneville Power Administration. Western is divided into four independent Customer Service Regions including the Sierra Nevada Region (Sierra Nevada), the focus of this report. The Central Valley Project (CVP) and the Washoe Project provide the primary power resources marketed by Sierra Nevada. Sierra Nevada also purchases and markets power generated by the Bonneville Power Administration, Pacific Gas and Electric (PG&E), and various power pools. Sierra Nevada currently markets approximately 1,480 megawatts of power to 77 customers in northern and central California. These customers include investor-owned utilities, public utilities, government agencies, military bases, and irrigation districts. Methods and conclusions from an economic analysis are summarized concerning distributional effects of alternative actions that Sierra Nevada could take with it`s new marketing plan.

  10. Analysis of the Alternating-current Circuits with Matlab%利用Matlab分析交流电路

    Institute of Scientific and Technical Information of China (English)

    陆朝华; 蓝海江

    2011-01-01

    探讨利用Matlab软件辅助分析交流电路的方法。以Matlab软件为辅助工具,具体分析了动态电路、正弦稳态电路及非正弦交流电路。结果表明,利用Matlab软件辅助分析交流电路问题,逻辑清晰、程序简短、结果精确。因此,Matlab软件是分析和研究电路的有效工具。%By using Matlab software,the aided analysis method of alternating-current(AC) circuits was explored.Taking Matlab software as aids,the dynamic circuit,sinusoidal steady state circuit and non-sinusoidal AC circuit were discussed.The results show that,it has such features: a clear logic,a short program and an accurate result,which analyzing the problems of AC circuits on using Matlab software.Therefore,the Matlab software is an effective tool for analyzing and researching AC circuits.

  11. Cost-benefit analysis of retrofit of high-intensity discharge factory lighting with energy-saving alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Preston, D.J. [Alabama Industrial Assessment Center, The University of Alabama, 1530 W. Tremont St, Allentown, PA 18102 (United States); Woodbury, K.A. [Alabama Industrial Assessment Center, The University of Alabama, 290 Hardaway Hall, Box 870276, Tuscaloosa, AL 35487-0276 (United States)

    2013-05-15

    Due to increased concern about overall energy costs and the appearance of efficient and inexpensive lighting system alternatives, factories and plants with high-intensity discharge (HID) lighting are forced to consider retrofit with more modern, energy-efficient lighting. The decision is complicated from an economic perspective, and there is a lack of information readily available on the topic. This study provides an analysis of the replacement by retrofit of common probe-start metal halide and high-pressure sodium industrial lighting systems. Retrofit options considered include the more recent pulse-start metal halide lamps and a range of T5 high output and T8 fluorescent lamp configurations. Recent data on lighting system pricing, labor and energy costs, and time required for tasks are reported. The results generated include savings, payback period, and net present value for many retrofit options, as well as the change in energy consumption, carbon footprint, and lumen output for each retrofit. Effects of varying rate of return and daily duration of operation are considered. Based on change in lumen output, payback period, net present value, and comparison of lighting quality, one or two options are recommended from the overall retrofit options considered. A fluorescent retrofit is recommended for each of the HID initial scenarios considered. The payback period is no more than 3 years in any recommended case. The focus of this study is on the potential energy and cost savings, and some proposed solutions may, or may not, be acceptable due to lack of illuminance uniformity.

  12. Alternative Treatments

    Science.gov (United States)

    ... Find your chapter: search by state Home > Alzheimer's Disease > Treatments > Alternative Treatments Overview What Is Dementia? What Is Alzheimer's? Younger/Early Onset Facts and Figures Know the 10 Signs Stages Inside the Brain: ...

  13. A Multi-Attribute Utility Decision Analysis for Treatment Alternatives for the DOE/SR Aluminum-Based Spent Nuclear Fuel

    Energy Technology Data Exchange (ETDEWEB)

    Davis, F.; Kuzio, K.; Sorenson, K.; Weiner, R.; Wheeler, T.

    1998-11-01

    A multi-attribute utility analysis is applied to the decision to select a treatment method for the management of aluminum-based spent nuclear i%el (A1-SNF) owned by the United States Department of Energy (DOE). DOE will receive, treat, and temporarily store Al- SNF, most of which is composed of highly enriched uranium, at its Savannah River Site in South Carolina. DOE intends ultimately to send the treated Al-SNJ? to a geologic repository for permanent disposal. DOE initially considered ten treatment alternatives for the management of A1-SNF, and has narrowed the choice to two of these the direct disposal and melt and dilute alternatives. The decision analysis presented in this document focuses on a decision between these two remaining alternatives.

  14. Dissecting an alternative splicing analysis workflow for GeneChip® Exon 1.0 ST Affymetrix arrays

    Directory of Open Access Journals (Sweden)

    Calogero Raffaele A

    2008-11-01

    Full Text Available Abstract Background A new microarray platform (GeneChip® Exon 1.0 ST has recently been developed by Affymetrix http://www.affymetrix.com. This microarray platform changes the conventional view of transcript analysis since it allows the evaluation of the expression level of a transcript by querying each exon component. The Exon 1.0 ST platform does however raise some issues regarding the approaches to be used in identifying genome-wide alternative splicing events (ASEs. In this study an exon-level data analysis workflow is dissected in order to detect limit and strength of each step, thus modifying the overall workflow and thereby optimizing the detection of ASEs. Results This study was carried out using a semi-synthetic exon-skipping benchmark experiment embedding a total of 268 exon skipping events. Our results point out that summarization methods (RMA, PLIER do not affect the efficacy of statistical tools in detecting ASEs. However, data pre-filtering is mandatory if the detected number of false ASEs are to be reduced. MiDAS and Rank Product methods efficiently detect true ASEs but they suffer from the lack of multiple test error correction. The intersection of MiDAS and Rank Product results efficiently moderates the detection of false ASEs. Conclusion To optimize the detection of ASEs we propose the following workflow: i data pre-filtering, ii statistical selection of ASEs using both MiDAS and Rank Product, iii intersection of results derived from the two statistical analyses in order to moderate family-wise errors (FWER.

  15. Nuclear proliferation and civilian nuclear power. Report of the Nonproliferation Alternative Systems Assessment Program. Volume V. Economics and systems analysis

    International Nuclear Information System (INIS)

    This NASAP assessment considers the economics of alternative nuclear reactor and fuel-cycle systems in the light of possible patterns of uranium supply and energy demand, as well as the economic implications of improvng the proliferation resistance of the various systems. The assessment focuses on the costs of alternative nuclear technologies and the possible timing of their implementation, based on their economic attractiveness

  16. Nuclear proliferation and civilian nuclear power. Report of the Nonproliferation Alternative Systems Assessment Program. Volume V. Economics and systems analysis

    Energy Technology Data Exchange (ETDEWEB)

    1980-06-01

    This NASAP assessment considers the economics of alternative nuclear reactor and fuel-cycle systems in the light of possible patterns of uranium supply and energy demand, as well as the economic implications of improvng the proliferation resistance of the various systems. The assessment focuses on the costs of alternative nuclear technologies and the possible timing of their implementation, based on their economic attractiveness.

  17. Productive Resources in Students' Ideas about Energy: An Alternative Analysis of Watts' Original Interview Transcripts

    Science.gov (United States)

    Harrer, Benedikt W.; Flood, Virginia J.; Wittmann, Michael C.

    2013-01-01

    For over 30 years, researchers have investigated students' ideas about energy with the intent of reforming instructional practice. In this pursuit, Watts contributed an influential study with his 1983 paper "Some alternative views of energy" ["Phys. Educ." 18, 213 (1983)]. Watts' "alternative frameworks"…

  18. ALTERNATIVE FUELS FOR DIESEL ENGINES

    Directory of Open Access Journals (Sweden)

    Jacek Caban

    2013-12-01

    Full Text Available This paper presents the development and genesis of the use of alternative fuels in internal combustion ignition engines. Based on the analysis of the literature, this article shows various alternative fuels used in Poland and all over the world. Furthermore, this article describes the research directions for alternative fuels use in road transport powered by diesel engines.

  19. ALTERNATIVE FUELS FOR DIESEL ENGINES

    OpenAIRE

    Jacek Caban; Agata Gniecka; Lukáš Holeša

    2013-01-01

    This paper presents the development and genesis of the use of alternative fuels in internal combustion ignition engines. Based on the analysis of the literature, this article shows various alternative fuels used in Poland and all over the world. Furthermore, this article describes the research directions for alternative fuels use in road transport powered by diesel engines.

  20. Low cost quantitative digital imaging as an alternative to qualitative in vivo bioassays for analysis of active aflatoxin B1.

    Science.gov (United States)

    Rasooly, Reuven; Do, Paula M; Hernlem, Bradley J

    2016-06-15

    Aflatoxin B1 (AFB1) producing fungi contaminate food and feed and are a major health concern. To minimize the sources and incidence of AFB1 illness there is a need to develop affordable, sensitive mobile devices for detection of active AFB1. In the present study we used a low cost fluorescence detector and describe two quantitative assays for detection of detoxified and active AFB1 demonstrating that AFB1 concentration can be measured as intensity of fluorescence. When the assay plate containing increasing concentrations of AFB1 is illuminated with a 366 nm ultraviolet lamp, AFB1 molecules absorb photons and emit blue light with peak wavelength of 432 nm. The fluorescence intensity increased in dose dependent manner. However, this method cannot distinguish between active AFB1 which poses a threat to health, and the detoxified AFB1 which exhibits no toxicity. To measure the toxin activity, we used a cell based assay that makes quantification more robust and is capable of detecting multiple samples simultaneously. It is an alternative to the qualitative duckling bioassay which is the "gold-standard" assay currently being used for quantitative analysis of active AFB1. AFB1 was incubated with transduced Vero cells expressing the green fluorescence protein (GFP) gene. After excitation with blue light at 475 nm, cells emitted green light with emission peak at 509 nm. The result shows that AFB1 inhibits protein expression in a concentration dependent manner resulting in proportionately less GFP fluorescence in cells exposed to AFB1. The result also indicates strong positive linear relationship with R(2)=0.90 between the low cost CCD camera and a fluorometer, which costs 100 times more than a CCD camera. This new analytical method for measuring active AFB1 is low in cost and combined with in vitro assay, is quantitative. It also does not require the use of animals and may be useful especially for laboratories in regions with limited resources.

  1. Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii.

    Science.gov (United States)

    Joerger, R D; Jacobson, M R; Premakumar, R; Wolfinger, E D; Bishop, P E

    1989-02-01

    The nucleotide sequence of a region of the Azotobacter vinelandii genome exhibiting sequence similarity to nifH has been determined. The order of open reading frames within this 6.1-kilobase-pair region was found to be anfH (alternative nitrogen fixation, nifH-like gene), anfD (nifD-like gene), anfG (potentially encoding a protein similar to the product of vnfG from Azotobacter chroococcum), anfK (nifK-like gene), followed by two additional open reading frames. The 5'-flanking region of anfH contains a nif promoter similar to that found in the A. vinelandii nifHDK gene cluster. The presumed products of anfH, anfD, and anfK are similar in predicted Mr and pI to the previously described subunits of nitrogenase 3. Deletion plus insertion mutations introduced into the anfHDGK region of wild-type strain A. vinelandii CA resulted in mutant strains that were unable to grow in Mo-deficient, N-free medium but grew in the presence of 1 microM Na2MoO4 or V2O5. Introduction of the same mutations into the nifHDK deletion strain CA11 resulted in strains that grew under diazotrophic conditions only in the presence of vanadium. The lack of nitrogenase 3 subunits in these mutant strains was demonstrated through two-dimensional gel analysis of protein extracts from cells derepressed for nitrogenase under Mo and V deficiency. These results indicate that anfH, anfD, and anfK encode structural proteins for nitrogenase 3. PMID:2644222

  2. Comparative morphometric analysis of Sorghum halepense plants growing on substrates of rock dump with addition of alternative fertilizers

    Directory of Open Access Journals (Sweden)

    Zoriana M. Beshley

    2014-04-01

    Full Text Available Morphometric parameters of Sorghum halepense (L. Pers. plants were identified and analyzed. The positive effect of alternative fertilizers on the plants growing on substrates of coal rock dump was established.

  3. Comparative morphometric analysis of Sorghum halepense plants growing on substrates of rock dump with addition of alternative fertilizers

    OpenAIRE

    Zoriana M. Beshley; Stephen V. Beshley; Baranov, Vladimir I.; Olga I. Terek

    2014-01-01

    Morphometric parameters of Sorghum halepense (L.) Pers. plants were identified and analyzed. The positive effect of alternative fertilizers on the plants growing on substrates of coal rock dump was established.

  4. 75 FR 59323 - Early Scoping for the Alternatives Analysis of the North Corridor Transit Project in Metropolitan...

    Science.gov (United States)

    2010-09-27

    ... defined transportation corridor, and for determining which particular investment strategy should be... maintenance costing, and environmental and land use analyses. Finally, costs, benefits, and impacts of each alternative are developed and evaluated, funding strategies are analyzed, and a locally preferred...

  5. Nuclear proliferation and civilian nuclear power: report of the Nonproliferation Alternative Systems Assessment Program. Volume V. Economics and systems analysis

    International Nuclear Information System (INIS)

    This assessment considers the economics of alternative nuclear reactor and fuel-cycle systems in the light of possible patterns of uranium supply and energy demand, as well as the economic implications of improving the proliferation resistance of the various systems. The assessment focuses on the costs of alternative nuclear technologies and the possible timing of their implementation, based on their economic attractiveness. The objectives of this assessment are to identify when economic incentives to deploy advanced nuclear power systems might exist, to estimate the costs of using technologies that would reduce the risk of proliferation, to assess the impact of major economic uncertainties on the transition to new technologies, and to compare the investments required for alternative systems. This information can then be used to assess the potential economic benefits of alternative research, development, and demonstration programs and the timing of those programs

  6. Cosmic alternatives?

    Science.gov (United States)

    Gregory, Ruth

    2009-04-01

    "Cosmologists are often in error but never in doubt." This pithy characterization by the Soviet physicist Lev Landau sums up the raison d'être of Facts and Speculations in Cosmology. Authors Jayant Narlikar and Geoffrey Burbidge are proponents of a "steady state" theory of cosmology, and they argue that the cosmological community has become fixated on a "Big Bang" dogma, suppressing alternative viewpoints. This book very much does what it says on the tin: it sets out what is known in cosmology, and puts forward the authors' point of view on an alternative to the Big Bang.

  7. Individual Characteristics and Stated Preferences for Alternative Energy Sources and Propulsion Technologies in Vehicles: A Discrete Choice Analysis

    OpenAIRE

    Andreas R. Ziegler

    2010-01-01

    This paper empirically examines the determinants of the demand for alternative energy sources and propulsion technologies in vehicles. The data stem from a stated preference discrete choice experiment with 598 potential car buyers. In order to simulate a realistic automobile purchase situation, seven alternatives were incorporated in each of the six choice sets, i.e. hybrid, gas, biofuel, hydrogen, and electric as well as the common fuels gasoline and diesel. The vehicle types were additional...

  8. Growing Alternatives

    DEFF Research Database (Denmark)

    Bagger-Petersen, Mai Corlin

    2014-01-01

    From 2014, Anhui Province will pilot a reform of the residential land market in China, thus integrating rural Anhui in the national housing market. In contrast, artist and activist Ou Ning has proposed the Bishan time money currency, intending to establish an alternative economic circuit in Bishan...

  9. Global analysis of CPSF2-mediated alternative splicing: Integration of global iCLIP and transcriptome profiling data

    Directory of Open Access Journals (Sweden)

    Ashish Misra

    2015-12-01

    Full Text Available Alternative splicing is a key mechanism for generating proteome diversity, however the mechanisms regulating alternative splicing are poorly understood. Using a genome-wide RNA interference screening strategy, we identified cleavage and polyadenylation specificity factor (CPSF and symplekin (SYMPK as cofactors of the well-known splicing regulator RBFOX2. To determine the role of CPSF in alternative splicing on a genome-wide level, we performed paired-end RNA sequencing (RNA-seq to compare splicing events in control cells and RBFOX2 or CPSF2 knockdown cells. We also performed individual-nucleotide resolution UV cross-linking and immunoprecipitation (iCLIP to identify direct binding targets of RBFOX2 and CPSF2. Here, we describe the experimental design, and the quality control and data analyses that were performed on the dataset. The raw sequencing data have been deposited in NCBI's Gene Expression Omnibus and are accessible through GEO Series accession number GSE60392.

  10. Profiling the Australian Consumer of Complementary and Alternative Medicine: A Secondary Analysis of National Health Survey Data.

    Science.gov (United States)

    Leach, Matthew J

    2016-07-01

    Background • Consumers' interest in complementary and alternative medicine (CAM) has escalated in the past few decades. Some observers argue that the changing needs and expectations of consumers are driving the surge. Although some studies support that notion, much of the research has been limited methodologically. Profiling can provide important insights into the distinct needs of CAM consumers. Objective • The study intended to profile consumers of CAM in Australia. Design • The study was a secondary analysis of 5 Australian National Health Surveys conducted between 1989 and 2008. Outcome Measures • The study measured the differences between CAM users and nonusers in terms of: (1) predisposing factors (ie, the prevailing conditions that predispose an individual to use a health service, such as age); (2) enabling factors (ie, circumstances that facilitate or hinder health service use, such as income); (3) need factors (ie, an actual or perceived need for health services, such as poor health); and (4) personal health practices (ie, behaviors that influence health status, such as alcohol consumption). Results • The 5 surveys provided data for 181 549 Australian adults and children. Predisposing factors associated with CAM use were (1) being aged >40 y, (2) being female, (3) being married, and (4) holding a postsecondary school qualification. Significant enablers of CAM use were (1) high income, (2) private health insurance, and (3) employment. As for personal health practices, CAM users had significantly higher odds of (1) being physically active, (2) being a nonsmoker, and (3) meeting national recommendations for intake of fruits and vegetables. The prevalence of chronic disease and the use of pharmaceutical agents and health services were comparatively high among CAM users. Conclusions • CAM consumers reported relatively healthier lifestyles compared with nonusers, although some data indicated that CAM users might have greater health care needs. The

  11. An economic analysis of alternative fertility control and associated management techniques for three BLM wild horse herds

    Science.gov (United States)

    Bartholow, John M.

    2004-01-01

    Contemporary cost projections were computed for several alternative strategies that could be used by BLM to manage three wild horse populations. The alternatives included existing gather and selective removal methods, combined with potential contraceptive applications of varying duration and other potentially useful management techniques. Costs were projected for a 20-year economic life using the Jenkins wild horse population model and cost estimates from BLM that reflect state-by-state per horse removal, adoption, long-term holding, and contraceptive application expenses. Important findings include: Application of currently available 2-year contraceptives appears capable of reducing variable operating costs for wild horse populations by about 21% on average.

  12. Orion Project: Alternate Attitude Study

    Science.gov (United States)

    Alvarez-Hernandez, A.; Miller, Stephen W.

    2009-01-01

    This slide presentation reviews the study done on alternate attitudes for the Orion project's crew exploration vehicle. The analysis focused on the thermal performance of the vehicle with the alternate attitudes. The pressure vessel heater power, other vehicle heaters and radiator sink temperatures were included in the analysis.

  13. Risk-based decision analysis of atmospheric emission alternatives to reduce ground water degradation on the European scale

    NARCIS (Netherlands)

    Wladis, D.; Rosen, L.; Kros, H.

    1999-01-01

    Environmental degradation due to emissions of sulfur dioxide, nitrate oxides, and ammonia from diffuse sources amounts to substantial costs to society and so do the alternatives to protect and restore the environment. Damage to ground water includes acidification, aluminum leaching, elevated concent

  14. An Alternate Method for Fourier Transform Infrared (FTIR) Spectroscopic Determination of Soil Nitrate Using Derivative Analysis and Sample Treatments

    NARCIS (Netherlands)

    Choe, E.; Meer, van der F.; Rossiter, D.; Salm, van der C.; Kim, K.W.

    2010-01-01

    This study aimed at examining effective sample treatments and spectral processing for an alternate method of soil nitrate determination using the attenuated total reflectance (ATR) of Fourier transform infrared (FTIR) spectroscopy. Prior to FTIR measurements, soil samples were prepared as paste to e

  15. 75 FR 32295 - Expedited Approval of Alternative Test Procedures for the Analysis of Contaminants Under the Safe...

    Science.gov (United States)

    2010-06-08

    ..., 2009, Federal Register notice (74 FR 57908) (USEPA 2009b). The corrected tables are titled: Alternative... methods approval process in an April 10, 2007, Federal Register notice (72 FR 17902) (USEPA 2007) and..., Federal Register notice (73 FR 31616) (USEPA 2008) and added appendix A to 40 CFR part 141, subpart...

  16. Immobilized High Level Waste (HLW) Interim Storage Alternative Generation and analysis and Decision Report 2nd Generation Implementing Architecture

    Energy Technology Data Exchange (ETDEWEB)

    CALMUS, R.B.

    2000-09-14

    Two alternative approaches were previously identified to provide second-generation interim storage of Immobilized High-Level Waste (IHLW). One approach was retrofit modification of the Fuel and Materials Examination Facility (FMEF) to accommodate IHLW. The results of the evaluation of the FMEF as the second-generation IHLW interim storage facility and subsequent decision process are provided in this document.

  17. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants

    OpenAIRE

    Teixeira da Silva, Jaime A.

    2015-01-01

    Abstract. Teixeira da Silva JA. 2015. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants. Nusantara Bioscience 7: 26-32. Syngonium podophyllum L. (arrowhead vine) is a popular leafy indoor pot plant whose tissue culture has been established, primarily through in vitro shoot culture, but several interesting aspects have not yet been explored. In this study, cv. ‘White Butterfly’ wa...

  18. Analysis of energy cogeneration with alternative motors in a brewery; Analise de cogeracao de energia com motores alternativos em uma cervejaria

    Energy Technology Data Exchange (ETDEWEB)

    Freitas, Evelyn Ramiro; Almeida Silvio Carlos Anibal de [Universidade Federal do Rio de Janeiro (DEM/UFRJ), RJ (Brazil). Dept. de Engenharia Mecanica], E-mail: eng_evelyn@poli.ufrj.br

    2010-07-01

    In this study it will be presented a technical-economical simulation on a plant that needs thermal and electrical energy, simultaneously. This simulation analysis will be based on the economical and technical validity of the installation of a thermo electrical cogeneration power plant using alternative motors. The objective intends the electrical autonomy of use of thermal energy and the better plant efficiency, and lower CO{sub 2} emissions. This analysis will utilize the Cogeneration Read Reckoner, version 3.1 of the Sinclair Knight Merz.

  19. Alternative crops

    International Nuclear Information System (INIS)

    Surplus cereal production in the EEC and decreasing product prices, mainly for cereals, has prompted considerable interest for new earnings in arable farming. The objective was to examine whether suggested new crops (fibre, oil, medicinal and alternative grains crops) could be considered as real alternatives. Whether a specific crop can compete economically with cereals and whether there is a market demand for the crop is analyzed. The described possibilities will result in ca. 50,000 hectares of new crops. It is expected that they would not immediately provide increased earnings, but in the long run expected price developments are more positive than for cereals. The area for new crops will not solve the current surplus cereal problem as the area used for new crops is only 3% of that used for cereals. Preconditions for many new crops is further research activities and development work as well as the establishment of processing units and organizational initiatives. Presumably, it is stated, there will then be a basis for a profitable production of new crops for some farmers. (AB) (47 refs.)

  20. Elimination of Whole Effluent Toxicity NPDES Permit Limits through the Use of an Alternative Testing Species and Reasonable Potential Analysis

    International Nuclear Information System (INIS)

    The cladoceran, Ceriodaphnia dubia (C. dubia), is required by the State of South Carolina to be used in whole effluent toxicity (WET) compliance tests in order to meet limits contained within National Pollutant Discharge Elimination System (NPDES) permits. Westinghouse Savannah River Company (WSRC) experienced WET test failures for no clear reason over a long period of time. Toxicity identification examinations on effluents did not indicate the presence of toxicants; therefore, the WET test itself was brought under suspicion. Research was undertaken with an alternate cladoceran, Daphnia ambigua (D. ambigua). It was determined that this species survives better in soft water, so approval was obtained from regulating authorities to use this ''alternate'' species in WET tests. The result was better test results and elimination of non-compliances. The successful use of D. ambigua allowed WSRC to gain approval from the South Carolina Department of Health and Environmental Control (SCDHEC) to remove WET limits from the NPDES permit

  1. Evaluation of Electronic Commerce (E-Commerce) with SWOT Analysis as an Alternative Network Marketing at Agricultural Marketing

    OpenAIRE

    Nuray Kızılaslan; Tayfur Ünal

    2015-01-01

    Compulsory competition occurring in the world trade has led the enterprises to different marketing system. Marketing problems seems to be a problem in Turkey rather than agricultural production problems. In this aspect, marketing alternatives are sought. E-commerce is a system with more opportunities in agricultural marketing. Increasing the applicability of this system in Turkey will eliminate many problems associated with marketing in agriculture. With an active use of E-commerce in agricul...

  2. Alternative Energies

    Energy Technology Data Exchange (ETDEWEB)

    Planting, A.; De saint Jacob, Y.; Verwijs, H.; Belin, H.; Preesman, L.

    2009-03-15

    In two articles, one interview and one column attention is paid to alternative energies. The article 'A new light on saving energy' discusses the option to save energy by modernising lighting systems in urban areas. The column 'View from Paris' focuses on investment decisions in France with regard to renewable energy and energy savings. The article 'Europe turns a blind eye to big battery' discusses developments in batteries to store energy. The interview concerns fuel cell expert and formerly President of UTC Power Jan van Dokkum. The last article gives a brief overview of the European Energy Research Alliance (EERA) and the challenges this alliance will have to face with regard to climate change and energy security.

  3. Energy alternatives

    International Nuclear Information System (INIS)

    English. A special committe of the Canadian House of Commons was established on 23 May 1980 to investigate the use of alternative energy sources such as 'gasohol', liquified coal, solar energy, methanol, wind and tidal power, biomass, and propane. In its final report, the committee envisions an energy system for Canada based on hydrogen and electricity, using solar and geothermal energy for low-grade heat. The committe was not able to say which method of generating electricty would dominate in the next century, although it recommends that fossil fuels should not be used. The fission process is not specifically discussed, but the outlook for fusion was investigated, and continued governmental support of fusion research is recommended. The report proposes some improvements in governmental energy organizations and programs

  4. 77 FR 38523 - Expedited Approval of Alternative Test Procedures for the Analysis of Contaminants Under the Safe...

    Science.gov (United States)

    2012-06-28

    ... Docket is (202) 566-2426. Abbreviations and Acronyms Used in This Action ANOVA: Analysis of Variance APHA... using random effects analysis of variance (ANOVA). The recovery limits were estimated at the...

  5. Teicoplanin as an effective alternative to vancomycin for treatment of MRSA infection in Chinese population: a meta-analysis of randomized controlled trials.

    Directory of Open Access Journals (Sweden)

    Yang Peng

    Full Text Available OBJECTIVE: To evaluate whether teicoplanin could be an alternative to vancomycin for treatment of MRSA infection in Chinese population using a meta-analysis in randomized controlled trials. METHODS: THE FOLLOWING DATABASES WERE SEARCHED: Chinese Biomedical Literature database (CBM, Chinese Journal Full-text database (CNKI, Wanfang database, Medline database, Ovid database and Cochrane Library. Articles published from 2002 to 2013 that studied teicoplanin in comparison to vancomycin in the treatment of MRSA infected patients were collected. Overall effects, publishing bias analysis and sensitivity analysis on clinical cure rate, microbiologic eradication rate and adverse events rate were performed by using Review Manager 5.2 and Stata 11.0 softwares. RESULTS: Twelve articles met entry criteria. There was no statistically significant difference between the two groups regarding the clinical cure rate (risk ratio [RR], teicoplanin vs vancomycin, 0.94; 95% CI, 0.74~1.19; P=0.60, microbiological cure rate (risk ratio [RR], teicoplanin vs vancomycin, 0.99; 95% CI, 0.91~1.07; P=0.74 and adverse event rate (risk ratio [RR], teicoplanin vs vancomycin, 0.86; 95% CI, 0.40~1.84; P=0.70. CONCLUSIONS: The meta-analysis results indicate that the two therapies are similar in both efficacy and safety, thus teicoplanin can act as an effective alternative to vancomycin for treating patients infected by MRSA.

  6. An energy-economic scenario analysis of alternative fuels for personal transport using the Global Multi-regional MARKAL model (GMM)

    Energy Technology Data Exchange (ETDEWEB)

    Guel, Timur; Kypreos, Socrates; Turton, Hal [Energy Economics Group, Paul Scherrer Institut, 5232 Villigen PSI (Switzerland); Barreto, Leonardo [Energy Economics Group, Paul Scherrer Institut, 5232 Villigen PSI (Switzerland); Energy Economics and Policy, Austrian Energy Agency, 1150 Vienna (Austria)

    2009-10-15

    This paper deals with the long-term prospects of alternative fuels in global personal transport. It aims at assessing key drivers and key bottlenecks for their deployment, focusing particularly on the role of biofuels and hydrogen in meeting climate policy objectives. The analysis is pursued using the Global Multi-regional MARKAL model (GMM), a perfect foresight ''bottom-up'' model of the global energy system with a detailed representation of alternative fuel chains, linked to the Model for the Assessment of Greenhouse Gas Induced Climate Change (MAGICC). The analysis shows that biofuels are limited by the regional availability of low-cost biomass, but can be important for meeting mild climate policy targets. If policy-makers intend to pursue more stringent climate policy, then hydrogen becomes a competitive option. However, the analysis finds that the use of hydrogen in personal transport is restricted to very stringent climate policy, as only such policy provides enough incentive to build up the required delivery infrastructure. An analysis of costs additionally shows that ''keeping the hydrogen option open'' does not take considerable investments compared to the investment needs in the power sector within the next decades, but allows the use of hydrogen for the pursuit of stringent climate policy in the second half of the century. (author)

  7. Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii.

    OpenAIRE

    Joerger, R D; Jacobson, M R; Premakumar, R; Wolfinger, E D; Bishop, P E

    1989-01-01

    The nucleotide sequence of a region of the Azotobacter vinelandii genome exhibiting sequence similarity to nifH has been determined. The order of open reading frames within this 6.1-kilobase-pair region was found to be anfH (alternative nitrogen fixation, nifH-like gene), anfD (nifD-like gene), anfG (potentially encoding a protein similar to the product of vnfG from Azotobacter chroococcum), anfK (nifK-like gene), followed by two additional open reading frames. The 5'-flanking region of anfH ...

  8. Alternative Green Solvents Project

    Science.gov (United States)

    Maloney, Phillip R.

    2012-01-01

    Necessary for safe and proper functioning of equipment. Mainly halogenated solvents. Tetrachloride, Trichloroethylene (TCE), CFC-113. No longer used due to regulatory/safety concerns. Precision Cleaning at KSC: Small % of total parts. Used for liquid oxygen (LOX) systems. Dual solvent process. Vertrel MCA (decafluoropentane (DFP) and trons-dichloroethylene) HFE-7100. DFP has long term environmental concerns. Project Goals: a) Identify potential replacements. b) 22 wet chemical processes. c) 3 alternative processes. d) Develop test procedures. e) Contamination and cleaning. f) Analysis. g) Use results to recommend alternative processes. Conclusions: a) No alternative matched Vertrel in this study. b) No clear second place solvent. c) Hydrocarbons- easy; Fluorinated greases- difficult. d) Fluorinated component may be needed in replacement solvent. e) Process may need to make up for shortcoming of the solvent. f) Plasma and SCC02 warrant further testing.

  9. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants

    Directory of Open Access Journals (Sweden)

    JAIME A. TEIXEIRA DA SILVA

    2015-05-01

    Full Text Available Abstract. Teixeira da Silva JA. 2015. Response of Syngonium podophyllum L. ‘White Butterfly’ shoot cultures to alternative media additives and gelling agents, and flow cytometric analysis of regenerants. Nusantara Bioscience 7: 26-32. Syngonium podophyllum L. (arrowhead vine is a popular leafy indoor pot plant whose tissue culture has been established, primarily through in vitro shoot culture, but several interesting aspects have not yet been explored. In this study, cv. ‘White Butterfly’ was used to investigate the response of shoot formation to alternative gelling agents and media additives. Gellan gum (Gelrite® at 2 g/L resulted in greater leaf production, plantlet fresh weight and higher chlorophyll content (SPAD value than all other gelling agents tested, including agar, Bacto agar, phytagel, oatmeal agar, potato dextrose agar, barley starch and corn starch, when on a basal Hyponex® (NPK = 6.5: 6: 19; 3 g/L medium. Several alternative liquid medium additives tested (low and full fat milk, Coca-Cola®, coffee, Japanese green, Oolong and Darjeeling teas negatively impacted plant growth, stunted roots and decreased chlorophyll content (SPAD value of leaves. Plant growth on medium with refined sucrose or table sugar responded similarly. Poor growth was observed when crude extract from a high rebaudioside-containing stevia (Stevia rebaudiana Bertoni line - an artificial sweetener - was used. Leaf tissue from the control did not show any endopolyploidy but low levels of endopolyploidy (8C were detected in some treatments.

  10. Accident analysis for high-level waste management alternatives in the US Department of Energy Environmental Restoration and Waste Management Programmatic Environmental Impact Statement

    International Nuclear Information System (INIS)

    A comparative generic accident analysis was performed for the programmatic alternatives for high-level waste (HLW) management in the US Department of Energy Environmental Restoration and Waste Management Programmatic Environmental Impact Statement (EM PEIS). The key facilities and operations of the five major HLW management phases were considered: current storage, retrieval, pretreatment, treatment, and interim canister storage. A spectrum of accidents covering the risk-dominant accidents was analyzed. Preliminary results are presented for HLW management at the Hanford site. A comparison of these results with those previously advanced shows fair agreement

  11. Alternative route for the MDW project for automotive fuels in the Dutch highway sector. A legal analysis

    International Nuclear Information System (INIS)

    In February 1998, the Dutch Government initiated an MDW project ('Marktwerking, Deregulering en Wetgevingskwaliteit', i.e. market functioning, deregulation and quality of legislation) to review ways in which competition within the motorway segment of the Dutch retail fuel market could be improved. The project reviewed the entry barriers and considered ways to stimulate competition, including preferential treatment of new entrants. The Dutch Major oil companies protested against the original plans of the Government and announced legal proceedings. The Government agreed to engage in negotiations with the oil industry in order to reach a compromise solution and avoid a legal battle. This resulted in an alternative route, which is analyzed in this article from a legal perspective

  12. Analysis of operations and cyber security policies for a system of cooperating Flexible Alternating Current Transmission System (FACTS) devices.

    Energy Technology Data Exchange (ETDEWEB)

    Phillips, Laurence R.; Tejani, Bankim; Margulies, Jonathan; Hills, Jason L.; Richardson, Bryan T.; Baca, Micheal J.; Weiland, Laura

    2005-12-01

    Flexible Alternating Current Transmission Systems (FACTS) devices are installed on electric power transmission lines to stabilize and regulate power flow. Power lines protected by FACTS devices can increase power flow and better respond to contingencies. The University of Missouri Rolla (UMR) is currently working on a multi-year project to examine the potential use of multiple FACTS devices distributed over a large power system region in a cooperative arrangement in which the FACTS devices work together to optimize and stabilize the regional power system. The report describes operational and security challenges that need to be addressed to employ FACTS devices in this way and recommends references, processes, technologies, and policies to address these challenges.

  13. Accident analysis for transuranic waste management alternatives in the U.S. Department of Energy waste management program

    International Nuclear Information System (INIS)

    Preliminary accident analyses and radiological source term evaluations have been conducted for transuranic waste (TRUW) as part of the US Department of Energy (DOE) effort to manage storage, treatment, and disposal of radioactive wastes at its various sites. The approach to assessing radiological releases from facility accidents was developed in support of the Office of Environmental Management Programmatic Environmental Impact Statement (EM PEIS). The methodology developed in this work is in accordance with the latest DOE guidelines, which consider the spectrum of possible accident scenarios in the implementation of various actions evaluated in an EIS. The radiological releases from potential risk-dominant accidents in storage and treatment facilities considered in the EM PEIS TRUW alternatives are described in this paper. The results show that significant releases can be predicted for only the most severe and extremely improbable accidents sequences

  14. Alaska Regional Energy Resources Planning Project. Phase 2: coal, hydroelectric and energy alternatives. Volume I. Beluga Coal District Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Rutledge, G.; Lane, D.; Edblom, G.

    1980-01-01

    This volume deals with the problems and procedures inherent in the development of the Beluga Coal District. Socio-economic implications of the development and management alternatives are discussed. A review of permits and approvals necessary for the initial development of Beluga Coal Field is presented. Major land tenure issues in the Beluga Coal District as well as existing transportation routes and proposed routes and sites are discussed. The various coal technologies which might be employed at Beluga are described. Transportation options and associated costs of transporting coal from the mine site area to a connecting point with a major, longer distance transportation made and of transporting coal both within and outside (exportation) the state are discussed. Some environmental issues involved in the development of the Beluga Coal Field are presented. (DMC)

  15. Analysis of climatic conditions and preliminary assessment of alternative cooling strategies for houses in California transition climate zones

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Y.J.; Zhang, H.

    1995-07-01

    This is a preliminary scoping study done as part of the {open_quotes}Alternatives to Compressive Cooling in California Transition Climates{close_quotes} project, which has the goal of demonstrating that houses in the transitional areas between the coast and the Central Valley of California do not require air-conditioning if they are properly designed and operated. The first part of this report analyzes the climate conditions within the transitional areas, with emphasis on design rather than seasonal conditions. Transitional climates are found to be milder but more variable than those further inland. The design temperatures under the most stringent design criteria, e.g. 0.1 % annual, are similar to those in the Valley, but significantly lower under more relaxed design criteria, e.g., 2% annual frequency. Transition climates also have large day-night temperature swings, indicating significant potential for night cooling, and wet-bulb depressions in excess of 25 F, indicating good potential for evaporative cooling. The second part of the report is a preliminary assessment using DOE-2 computer simulations of the effectiveness of alternative cooling and control strategies in improving indoor comfort conditions in two conventional Title-24 houses modeled in various transition climate locations. The cooling measures studied include increased insulation, light colors, low-emissivity glazing, window overhangs, and exposed floor slab. The control strategies studied include natural and mechanical ventilation, and direct and two-stage evaporative cooling. The results indicate the cooling strategies all have limited effectiveness, and need to be combined to produce significant improvements in indoor comfort. Natural and forced ventilation provide similar improvements in indoor conditions, but during peak cooling periods, these will still be above the comfort zone. Two-stage evaporative coolers can maintain indoor comfort at all hours, but not so direct evaporative coolers.

  16. Development and validation of an alternative to conventional pretreatment methods for residue analysis of butachlor in water, soil, and rice.

    Science.gov (United States)

    Xue, Jiaying; Jiang, Wenqing; Liu, Fengmao; Zhao, Huiyu; Wang, Suli; Peng, Wei

    2014-01-01

    A rapid and effective alternative analytical method for residues of butachlor in water, soil, and rice was established. The operating variables affecting performance of this method, including different extraction conditions and cleanup adsorbents, were evaluated. The determination of butachlor residues in soil, straw, rice hull, and husked rice was performed using GC/MS after extraction with n-hexane and cleanup with graphite carbon black. The average recoveries ranged from 81.5 to 102.7%, with RSDs of 0.6-7.7% for all of the matrixes investigated. The limits of quantitation were 0.05 mg/kg in water and rice plant, and 0.01 mg/kg in soil, straw, rice hull, and husked rice. A comparison among this proposed method, the conventional liquid-liquid extraction, the Quick, Easy, Cheap, Effective, Rugged, and Safe method, and Soxhlet extraction indicated that this method was more suitable for analyzing butachlor in rice samples. The further validation of the proposed method was carried out by Soxhlet extraction for the determination of butachlor residues in the husked rice samples, and the residue results showed there was no obvious difference obtained from these two methods. Samples from a rice field were found to contain butachlor residues below the maximum residue limits set by China (0.5 mg/kg) and Japan (0.1 mg/kg). The proposed method has a strong potential for application in routine screening and processing of large numbers of samples. This study developed a more effective alternative to the conventional analytical methods for analyzing butachlor residues in various matrixes.

  17. Analysis of an alternative method for the study of bromeliad-associated fauna in plants with different foliar organization

    Directory of Open Access Journals (Sweden)

    Gerson A. Müller

    2010-12-01

    Full Text Available The efficiency of an alternative method of collection (by suction of water for the study of Culicidae and Chironomidae (Diptera, Scirtidae (Coleoptera and Coenagrionidae (Odonata in bromeliads with different foliar architecture in a restinga at Florianópolis, SC, Brazil, was studied. The alternative method was less efficient to collect Culicidae and Chironomidae (Wilcoxon test p 0.05 from Aechmea lindenii. This method was less efficient to collect insects of all groups from Vriesea friburgensis (Wilcoxon test p A eficiência do método alternativo de coleta (por sucção da água para o estudo de Culicidae e Chironomidae (Diptera, Scirtidae (Coleoptera e Coenagrionidae (Odonata em bromélias com diferentes estruturas foliares de restinga em Florianópolis, SC, Brasil, foi estudada. O método alternativo foi menos eficiente para coletar Culicidae e Chironomidae (teste de Wilcoxon p 0, 05 a partir de Aechmea lindenii. Esse foi menos eficiente para coletar insetos de todos os grupos a partir de Vriesea friburgensis (teste de Wilcoxon p < 0,05. O método alternativo se mostrou eficiente em estimar a diversidade desses insetos nas duas espécies de bromélias. A alta mobilidade das formas imaturas dos coleópteros e libélulas e a disponibilidade de apenas um tanque em Aechea lindenii, em contraste com as várias axilas e Vriesea friburgensis, facilitando a sucção destas formas imaturas provavelmente influenciaram os resultados. Os resultados indicam que o método de sucção não deve substituir o desmanche no estudo de Culicidae e Chironomidae; ele pode ser útil para a obtenção de formas imaturas de Scirtidae e Coenagrionidae em bromélias de um só tanque.

  18. On the Distance Dependence of the Price Elasticity of Telecommunications Demand; Meta-analysis, and Alternative Theoretical Backgrounds

    NARCIS (Netherlands)

    Ouwersloot, Hans; Rietveld, Piet

    1997-01-01

    The positive correlation between the absolute price elasticity of telecommunications demand and the distance of the calling relation is well known. In this paper we first present a meta-analysis of existing studies to buttress the distance dependence empirically. The analysis confirms the existence

  19. 基于专利分析的替代性技术识别研究%Identification of Alternative Technology Based on Patent Analysis

    Institute of Scientific and Technical Information of China (English)

    娄岩; 张赏; 黄鲁成; 苗红

    2014-01-01

    The identification of alternative technologies is the basis for technology strategy choice of enterprise. Currently, the research on alternative technologies is mostly confined to qualitative studies and lacks an exact quantitative method, much less uses the method of pa-tent analysis. After summarizing the characteristics of alternative technologies, this paper presents the identification framework from two aspects:core feature and similarity. It uses an integrated approach combining of text mining, co-occurrence of manual code and use&ad-vantage matrix to identify the alternative technologies progressively. The empirical research on baseband chip technology proves the feasi-bility of the suggested approach.%替代性技术识别是企业进行技术战略选择的基础。目前,对替代性技术识别的研究多集中于定性分析,很少采用定量方法,利用专利文献与技术的关联关系进行替代性技术识别的研究少之更少。笔者在总结了替代性技术特征的基础上,构建了替代性技术识别方法框架,从核心性和相似性两个视角,综合利用文本挖掘、德温特手工代码共现、应用功效矩阵等方法逐层递进式识别替代性技术。以基带芯片技术为研究对象,进行实际论证,验证了该方法的可行性。

  20. Alternative Splice in Alternative Lice.

    Science.gov (United States)

    Tovar-Corona, Jaime M; Castillo-Morales, Atahualpa; Chen, Lu; Olds, Brett P; Clark, John M; Reynolds, Stuart E; Pittendrigh, Barry R; Feil, Edward J; Urrutia, Araxi O

    2015-10-01

    Genomic and transcriptomics analyses have revealed human head and body lice to be almost genetically identical; although con-specific, they nevertheless occupy distinct ecological niches and have differing feeding patterns. Most importantly, while head lice are not known to be vector competent, body lice can transmit three serious bacterial diseases; epidemictyphus, trench fever, and relapsing fever. In order to gain insights into the molecular bases for these differences, we analyzed alternative splicing (AS) using next-generation sequencing data for one strain of head lice and one strain of body lice. We identified a total of 3,598 AS events which were head or body lice specific. Exon skipping AS events were overrepresented among both head and body lice, whereas intron retention events were underrepresented in both. However, both the enrichment of exon skipping and the underrepresentation of intron retention are significantly stronger in body lice compared with head lice. Genes containing body louse-specific AS events were found to be significantly enriched for functions associated with development of the nervous system, salivary gland, trachea, and ovarian follicle cells, as well as regulation of transcription. In contrast, no functional categories were overrepresented among genes with head louse-specific AS events. Together, our results constitute the first evidence for transcript pool differences in head and body lice, providing insights into molecular adaptations that enabled human lice to adapt to clothing, and representing a powerful illustration of the pivotal role AS can play in functional adaptation. PMID:26169943

  1. On the Distance Dependence of the Price Elasticity of Telecommunications Demand; Meta-analysis, and Alternative Theoretical Backgrounds

    OpenAIRE

    Ouwersloot, Hans; Rietveld, Piet

    1997-01-01

    The positive correlation between the absolute price elasticity of telecommunications demand and the distance of the calling relation is well known. In this paper we first present a meta-analysis of existing studies to buttress the distance dependence empirically. The analysis confirms the existence of distance dependence, and gives insight into the size of the effect.Next we look for various explanations of the distance dependence. We analyse the roles of the functional form of demand functio...

  2. Complement factor D homolog involved in the alternative complement pathway of rock bream (Oplegnathus fasciatus): Molecular and functional characterization and immune responsive mRNA expression analysis.

    Science.gov (United States)

    Godahewa, G I; Perera, N C N; Bathige, S D N K; Nam, Bo-Hye; Noh, Jae Koo; Lee, Jehee

    2016-08-01

    The complement system serves conventional role in the innate defense against common invading pathogens. Complement factor D (CfD) is vital to alternative complement pathway activation in cleaving complement factor B. This catalytic reaction forms the alternative C3 convertase that is crucial for complement-mediated pathogenesis. In this study, rock bream (Oplegnathus fasciatus) CfD (OfCfD) was characterized and OfCfD mRNA expression was investigated. OfCfD encodes 277 amino acids (aa) for a 30-kDa polypeptide. A domain analysis of the deduced OfCfD aa sequence showed a single serine protease trypsin superfamily domain, a serine active region, three active sites, and three substrate-binding sites. Pairwise sequence comparisons indicated that OfCfD has the highest identity (84.5%) with Oreochromis niloticus CfD. The phylogenetic tree revealed a common ancestral origin of CfD members, with fish CfD distinct from other vertebrate orthologs. The structural arrangement of the OfCfD gene (2451 bp) contained five exons interrupted by four introns. A spatial transcriptional analysis indicated that OfCfD transcripts constitutively expressed in all of the examined rock bream tissues, and that they were highest in the spleen and liver. In addition, OfCfD transcripts were immunologically upregulated by lipopolysaccharide (LPS) (12 h p.i.), Streptococcus iniae (12 h p.i.), rock bream iridovirus (RBIV) (6-12 h p.i.), and poly I:C (6 h p.i.) in spleen tissue. OfCfD is a trypsin protease and its recombinant protein showed strong protease activity similar to that of trypsin, indicating its catalytic function in the alternative pathway. Together, our findings suggest that OfCfD might be involved in immune responses in rock bream. PMID:27311435

  3. Looking for an Alternative.

    Science.gov (United States)

    Kennedy, Jack

    1999-01-01

    Argues that high school newspapers might do well to create stronger ties with alternative weeklies. Discusses issues of niche marketing, alternative content, and alternative presentation. Notes that high school papers could learn a lot from alternative newspapers. (SR)

  4. Economic analysis of the use of coronary calcium scoring as an alternative to stress ECG in the non-invasive diagnosis of coronary artery disease

    Energy Technology Data Exchange (ETDEWEB)

    Raman, Vivek [Brighton and Sussex University Hospitals, Brighton (United Kingdom); Royal Sussex County Hospital, Brighton (United Kingdom); McWilliams, Eric T.M. [Conquest Hospital, Hastings (United Kingdom); Holmberg, Stephen R.M.; Miles, Ken [Brighton and Sussex University Hospitals, Brighton (United Kingdom)

    2012-03-15

    To conduct an economic analysis (EA) of coronary calcium scoring (CCS) using a 0 score, as alternative to stress electrocardiography (sECG) in diagnosing coronary artery disease (CAD). A decision tree was constructed to compare four strategies for investigation of suspected CAD previously assessed in the formulation of clinical guidelines for the United Kingdom (UK) to two new strategies incorporating CCS. Sensitivity (96%; 95% CI 95.4-96.4%) and specificity (40%; 95% CI 38.7-41.4%) values for CCS were derived from a meta-analysis of 10,760 patients. Other input variables were obtained from a previous EA and average prices for hospital procedures in the UK. A threshold of pound 30,000/Quality-adjusted Life Year (QALY) was considered cost-effective. Using net monetary benefit calculations, CCS-based strategies were found to be cost-effective compared to sECG equivalents at all assessed prevalence of CAD. Using CCS prior to myocardial perfusion scintigraphy (MPS) and catheter angiography (CA) was found to be cost-effective at pre-test probabilities (PTP) below 30%. Adoption of CCS as an alternative to sECG in investigating suspected stable angina in low PTP population (<30%) would be cost-effective. In patients with PTP of CAD >30%, proceeding to MPS or CA would be more cost-effective than performing either CCS or sECG. (orig.)

  5. Analysis of time-variant quadratic phase couplings in the tracé alternant EEG by recursive estimation of 3rd-order time-frequency distributions.

    Science.gov (United States)

    Helbig, Marko; Schwab, Karin; Leistritz, Lutz; Eiselt, Michael; Witte, Herbert

    2006-10-15

    The quantification of transient quadratic phase couplings (QPC) by means of time-variant bispectral analysis is a useful approach to explain several interrelations between signal components. A generalized recursive estimation approach for 3rd-order time-frequency distributions (3rd-order TFD) is introduced. Based on 3rd-order TFD, time-variant estimations of biamplitude (BA), bicoherence (BC) and phase bicoherence (PBC) can be derived. Different smoothing windows and local moment functions for an optimization of the estimation properties are investigated and compared. The methods are applied to signal simulations and EEG signals, and it can be shown that the new time-variant bispectral analysis results in a reliable quantification of QPC in the tracé alternant EEG of healthy neonates. PMID:16737739

  6. Simultaneous quantitative analysis of olmesartan, amlodipine and hydrochlorothiazide in their combined dosage form utilizing classical and alternating least squares based chemometric methods

    Directory of Open Access Journals (Sweden)

    Darwish Hany W.

    2016-03-01

    Full Text Available Simultaneous spectrophotometric analysis of a multi-component dosage form of olmesartan, amlodipine and hydrochlorothiazide used for the treatment of hypertension has been carried out using various chemometric methods. Multivariate calibration methods include classical least squares (CLS executed by net analyte processing (NAP-CLS, orthogonal signal correction (OSC-CLS and direct orthogonal signal correction (DOSC-CLS in addition to multivariate curve resolution-alternating least squares (MCR-ALS. Results demonstrated the efficiency of the proposed methods as quantitative tools of analysis as well as their qualitative capability. The three analytes were determined precisely using the aforementioned methods in an external data set and in a dosage form after optimization of experimental conditions. Finally, the efficiency of the models was validated via comparison with the partial least squares (PLS method in terms of accuracy and precision.

  7. Evaluation of Electronic Commerce (E-Commerce with SWOT Analysis as an Alternative Network Marketing at Agricultural Marketing

    Directory of Open Access Journals (Sweden)

    Nuray Kızılaslan

    2015-07-01

    Full Text Available Compulsory competition occurring in the world trade has led the enterprises to different marketing system. Marketing problems seems to be a problem in Turkey rather than agricultural production problems. In this aspect, marketing alternatives are sought. E-commerce is a system with more opportunities in agricultural marketing. Increasing the applicability of this system in Turkey will eliminate many problems associated with marketing in agriculture. With an active use of E-commerce in agricultural marketing, it provides convenience at marketing products of agro-related industries agencies and producers and all country will have a potential market position. In this study, possibilities offered by e-commerce to the agricultural sector, opportunities, threats, deficiencies and contributions to agricultural sector have been addressed. Without the limitations of place and time via the internet and computers, establishing national and international supply and demand balance of e-commerce seems to play important roles in maintaining an active and alive marketing. Furthermore, it is an important tool in reducing agricultural marketing problems.

  8. Optimization and analysis of alternative trapezoidal labyrinth weir; Otimizacao e analise de alternativas de vertedor do tipo Labirinto trapezoidal

    Energy Technology Data Exchange (ETDEWEB)

    Roselli, Rafael Gustavo [Universidade de Sao Paulo (USP), SP (Brazil)], email: rafaelroselli@yahoo.com.br

    2011-04-15

    This paper proposes solutions based on an analysis developed in spillway structures, involving analysis of the criteria for the design of labyrinth weirs. For this purpose, it is necessary to study and compare results to be developed in each project with respect to hydraulic factors, hydrological, geological, topographical and involving risks of losses caused by failures in hydraulic structures. The proposition of optimizing the arrangement of the spillway structure type labyrinth is designed to improve the safety of the construction and of the operation, focusing on the development of the ideal spillway for the case study. (author)

  9. Life cycle analysis and choice of natural gas-based automotive alternative fuels in Chongqing Municipality,China

    Institute of Scientific and Technical Information of China (English)

    WU Rui; LI Guangyi; ZHANG Zongyi; REN Yulong; HAN Weijian

    2007-01-01

    Road transport produces significant amounts of emissions by using crude oil as the primary energy source.A reduction of emissions can be achieved by implementing alternative fuel chains.The objective of this study is to carry out an economic,environmental and energy (EEE) life cycle study on natural gas-based automotive fuels with conventional gasoline in an abundant region of China.A set of indices of four fuels/vehicle systems on the basis of life cycle are assessed in terms of impact of EEE,in which natural gas produces compressed natural gas (CNG),methanol,dimethylether (DME) and Fischer Tropsch diesel (FTD).The study included fuel production,vehicle production,vehicle operation,infrastructure and vehicle end of life as a system for each fuel/vehicle system.A generic gasoline fueled car is used as a baseline.Data have been reviewed and modified based on the best knowledge available to Chongqing local sources.Results indicated that when we could not change electric and hydrogen fuel cell vehicles into commercial vehicles on a large scale,direct use of CNG in a dedicated or bi-fuel vehicle is an economical choice for the region which is most energy efficient and more environmental friendly.The study can be used to support decisions on how natural gas resources can best be utilized as a fuel/energy resource for automobiles,and what issues need to be resolved in Chongqing.The models and approaches for this study can be applied to other regions of China as long as all the assumptions are well defined and modified to find a substitute automotive energy source and establish an energy policy in a specific region.

  10. The cost-effectiveness of neonatal screening for Cystic Fibrosis: an analysis of alternative scenarios using a decision model

    Directory of Open Access Journals (Sweden)

    Tu Karen

    2005-08-01

    Full Text Available Abstract Background The use of neonatal screening for cystic fibrosis is widely debated in the United Kingdom and elsewhere, but the evidence available to inform policy is limited. This paper explores the cost-effectiveness of adding screening for cystic fibrosis to an existing routine neonatal screening programme for congenital hypothyroidism and phenylketonuria, under alternative scenarios and assumptions. Methods The study is based on a decision model comparing screening to no screening in terms of a number of outcome measures, including diagnosis of cystic fibrosis, life-time treatment costs, life years and QALYs gained. The setting is a hypothetical UK health region without an existing neonatal screening programme for cystic fibrosis. Results Under initial assumptions, neonatal screening (using an immunoreactive trypsin/DNA two stage screening protocol costs £5,387 per infant diagnosed, or £1.83 per infant screened (1998 costs. Neonatal screening for cystic fibrosis produces an incremental cost-effectiveness of £6,864 per QALY gained, in our base case scenario (an assumed benefit of a 6 month delay in the emergence of symptoms. A difference of 11 months or more in the emergence of symptoms (and mean survival means neonatal screening is both less costly and produces better outcomes than no screening. Conclusion Neonatal screening is expensive as a method of diagnosis. Neonatal screening may be a cost-effective intervention if the hypothesised delays in the onset of symptoms are confirmed. Implementing both antenatal and neonatal screening would undermine potential economic benefits, since a reduction in the birth incidence of cystic fibrosis would reduce the cost-effectiveness of neonatal screening.

  11. An alternative method for noise analysis using pixel variance as part of quality control procedures on digital mammography systems.

    NARCIS (Netherlands)

    Bouwman, R.; Young, K.; Lazzari, B.; Ravaglia, V.; Broeders, M.J.M.; Engen, R. van

    2009-01-01

    According to the European Guidelines for quality assured breast cancer screening and diagnosis, noise analysis is one of the measurements that needs to be performed as part of quality control procedures on digital mammography systems. However, the method recommended in the European Guidelines does n

  12. A Legitimate Case of Component Analysis of Ipsative Measures, and Partialling the Mean as an Alternative to Ipsatization

    NARCIS (Netherlands)

    Ten Berge, J.M.F.

    1999-01-01

    Ipsatizing variables prior to component analysis, by subtracting the mean score of each individual from all scores of that individual, has met with serious criticisms, both strategic and technical. On the other hand, the procedure is still popular in the study of personality, as a means of removing

  13. An Analysis of Fiscal Policy Alternatives for Serving Special Populations in Vocational Education. Information Series No. 278.

    Science.gov (United States)

    Phelps, L. Allen

    This policy analysis focuses on identifying optimal federal fiscal policies for achieving desired programmatic and student outcomes on behalf of special populations in vocational education (i.e., disadvantaged, limited-English-proficient, and handicapped persons, and students interested in nontraditional careers). The present status of unserved…

  14. A comparison of analytic hierarchy process and conjoint analysis methods in assessing treatment alternatives for stroke rehabilitation

    NARCIS (Netherlands)

    IJzerman, M.J.; Til, van J.A.; Bridges, J.F.P.

    2012-01-01

    Background: With growing emphasis on patient involvement in health technology assessment, there is a need for scientific methods that formally elicit patient preferences. Analytic hierarchy process (AHP) and conjoint analysis (CA) are two established scientific methods – albeit with very different o

  15. Synchrotron radiation induced x-ray micro analysis: A realistic alternative for electron- and ion beam microscopy?

    International Nuclear Information System (INIS)

    Synchrotron Radiation induced X-ray micro Fluorescence analysis (μ-SRXRF) is compared with more conventional microanalytical techniques such as Secondary Ion Microscopy (SIMS) and Electron Probe X-ray Microanalysis (EPXMA) for two typical microanalytical applications. SRXRF and EPXMA are employed for the analysis of individual particles, showing the complementary character of both techniques. By means of element mapping of trace constituents in a heterogeneous feldspar, the strong and weak points of SRXRF in comparison to EPXMA and SIMS are illustrated. The most striking difference between SRXRF and the other two microanalytical methods is the ability of SRXRF to probe deep into the investigated Material, whereas SIMS and EPXMA only investigate the upper surface of the material. The possibilities of SRXRF at third generation synchrotron rings is also briefly discussed

  16. Clinical outcomes with alternative dosing strategies for piperacillin/tazobactam: a systematic review and meta-analysis.

    Directory of Open Access Journals (Sweden)

    Hui Yang

    Full Text Available A better dosing strategy can improve clinical outcomes for patients. We sought to compare the extended or continuous infusion with conventional intermittent infusion of piperacillin/tazobactam, investigating which approach is better and worthy of recommendation for clinical use.Articles were gathered from PubMed, Web of Science, ProQuest, Science Direct, Cochrane, two Chinese literature databases (CNKI, Wan Fang Data and related ICAAC and ACCP conferences. Randomized controlled and observational studies that compared extended or continuous infusion with conventional intermittent infusion of piperacillin/tazobactam were identified from the databases above and analyzed. Two reviewers independently extracted and investigated the data. A meta-analysis was performed using Revman 5.2 software. The quality of each study was assessed. Sensitivity analysis and publication bias were evaluated.Five randomized controlled trials and nine observational studies were included in this study. All included studies had high quality and no publication bias was found. Compared to the conventional intermittent infusion approach, the extended or continuous infusion group had a significantly higher clinical cure rate (OR 1.88, 95% CI 1.29-2.73, P = 0.0009 and a lower mortality rate (OR 0.67, 95% CI 0.50-0.89, P = 0.005. No statistical difference was observed for bacteriologic cure (OR 1.40, 95% CI 0.82-2.37, P = 0.22 between the two dosing regimens. The sensitivity analysis showed the results were stable.Our systematic review and meta-analysis suggested that the extended or continuous infusion strategy of piperacillin/tazobactam should be recommended for clinical use considering its higher clinical cure rate and lower mortality rate in comparison with conventional intermittent strategy. Data from this study could be extrapolated for other β-lactam antimicrobials. Therefore, this dosing strategy could be considered in clinical practice.

  17. Thermodynamic Analysis of Actual Vapour Compression System with R12 and Its Eco-Friendly Alternatives Refrigerants

    OpenAIRE

    Vijay Singh Bisht; A.K.Pratihar

    2014-01-01

    This paper presents a theoretical performance study of a vapour compression refrigeration system with refrigerants R-12, R134a and R1234yf. A computational model based on energy first law analysis is presented for the investigation of the effects of evaporating temperatures, degree of subcooling, dead state temperatures and effectiveness of the liquid vapour heat exchanger on the relative capacity change index, coefficient of performance of the vapour compression refrigeration cycle. RCI (rel...

  18. Stochastic dominance analysis of on-farm-trial data: The riskiness of alternative phosphate sources in Burkina Faso

    OpenAIRE

    Hien, Victor; Kabore, Daniel; Youl, Sansan; Lowenberg-DeBoer, J.

    1997-01-01

    Stochastic dominance was used to determine the risk characteristics of phosphate fertilization of millet, sorghum and maize with commercial NPK fertilizer, rock phosphate and partially acidulated rock phosphate in Burkina Faso. On-farm-trial data from 1989, 1990 and 1991 in three rainfall zones was used. The analysis shows that among the four treatments tested, commercial NPK fertilizer has the most desirable risk characteristics. It is acceptable to risk averse decision makers for all three ...

  19. lociNGS: A Lightweight Alternative for Assessing Suitability of Next-Generation Loci for Evolutionary Analysis

    OpenAIRE

    Hird, Sarah M.

    2012-01-01

    Genomic enrichment methods and next-generation sequencing produce uneven coverage for the portions of the genome (the loci) they target; this information is essential for ascertaining the suitability of each locus for further analysis. lociNGS is a user-friendly accessory program that takes multi-FASTA formatted loci, next-generation sequence alignments and demographic data as input and collates, displays and outputs information about the data. Summary information includes the parameters cove...

  20. Continuous Wavelet Transform, a powerful alternative to Derivative Spectrophotometry in analysis of binary and ternary mixtures: A comparative study.

    Science.gov (United States)

    Elzanfaly, Eman S; Hassan, Said A; Salem, Maissa Y; El-Zeany, Badr A

    2015-12-01

    A comparative study was established between two signal processing techniques showing the theoretical algorithm for each method and making a comparison between them to indicate the advantages and limitations. The methods under study are Numerical Differentiation (ND) and Continuous Wavelet Transform (CWT). These methods were studied as spectrophotometric resolution tools for simultaneous analysis of binary and ternary mixtures. To present the comparison, the two methods were applied for the resolution of Bisoprolol (BIS) and Hydrochlorothiazide (HCT) in their binary mixture and for the analysis of Amlodipine (AML), Aliskiren (ALI) and Hydrochlorothiazide (HCT) as an example for ternary mixtures. By comparing the results in laboratory prepared mixtures, it was proven that CWT technique is more efficient and advantageous in analysis of mixtures with severe overlapped spectra than ND. The CWT was applied for quantitative determination of the drugs in their pharmaceutical formulations and validated according to the ICH guidelines where accuracy, precision, repeatability and robustness were found to be within the acceptable limit. PMID:26186613

  1. Exon Array Analysis using re-defined probe sets results in reliable identification of alternatively spliced genes in non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    Gröne Jörn

    2010-11-01

    Full Text Available Abstract Background Treatment of non-small cell lung cancer with novel targeted therapies is a major unmet clinical need. Alternative splicing is a mechanism which generates diverse protein products and is of functional relevance in cancer. Results In this study, a genome-wide analysis of the alteration of splicing patterns between lung cancer and normal lung tissue was performed. We generated an exon array data set derived from matched pairs of lung cancer and normal lung tissue including both the adenocarcinoma and the squamous cell carcinoma subtypes. An enhanced workflow was developed to reliably detect differential splicing in an exon array data set. In total, 330 genes were found to be differentially spliced in non-small cell lung cancer compared to normal lung tissue. Microarray findings were validated with independent laboratory methods for CLSTN1, FN1, KIAA1217, MYO18A, NCOR2, NUMB, SLK, SYNE2, TPM1, (in total, 10 events and ADD3, which was analysed in depth. We achieved a high validation rate of 69%. Evidence was found that the activity of FOX2, the splicing factor shown to cause cancer-specific splicing patterns in breast and ovarian cancer, is not altered at the transcript level in several cancer types including lung cancer. Conclusions This study demonstrates how alternatively spliced genes can reliably be identified in a cancer data set. Our findings underline that key processes of cancer progression in NSCLC are affected by alternative splicing, which can be exploited in the search for novel targeted therapies.

  2. Molecular characterization, alternative splicing and expression analysis of ACSF2 and its correlation with egg-laying performance in geese.

    Science.gov (United States)

    Yu, S; Wei, W; Xia, M; Jiang, Z; He, D; Li, Z; Han, H; Chu, W; Liu, H; Chen, J

    2016-08-01

    ACSF2 (encoded by acyl-CoA synthetase family member 2) belongs to the acyl-CoA synthetase (ACS) family, activating fatty acids by forming a thioester bond with CoA. In our previous study, a SNP residing in the intron of ACSF2 was identified to be linked to goose egg-laying performance. But the structure of goose ACSF2 as well as its role in reproduction remains unknown. In this study, we cloned and characterized ACSF2 in Yangzhou geese. A total of four alternative splice variants, designated as ACSF2-1, ACSF2-2, ACSF2-3 and ACSF2-4 respectively, were identified in the ovary. The coding regions of the four variants are 1770, 1692, 1599 and 1917 bp in length, respectively encoding 589, 563, 532 and 638 amino acids with conserved AMP-binding sites. All ACSF2 variants were widely expressed in 11 tested tissues in geese, except that the ACSF2-2 transcript was not detected in hypothalamus, pituitary gland and granulosa cells. Subcellular localization revealed that ACSF2 is a mitochondrial matrix protein. ACSF2 mRNA level was compared between high egg production (HEP; n = 8) and low egg production (LEP; n = 10) groups and showed a lower (P < 0.05) mRNA level in the HEP group. Further experiments indicated that overexpressing ACSF2 resulted in a significant increase of caspase-3 mRNA levels and that ACSF2 knockdown triggered a decrease in the caspase-3 mRNA level in granulosa cells. Similarly, the lower caspase-3 mRNA levels were identified in ovaries of the HEP group with lower ACSF2 mRNA levels. The research showed that the ACSF2 mRNA levels had a positive correlation with caspase-3 mRNA levels in vivo (R = 0.86, P < 0.01). Our results suggest that lower ACSF2 expression promotes the laying performance of goose possibly by inhibiting granulosa cell apoptosis and facilitating follicular development. PMID:27062512

  3. [Alternatives to animal testing].

    Science.gov (United States)

    Fabre, Isabelle

    2009-11-01

    The use of alternative methods to animal testing are an integral part of the 3Rs concept (refine, reduce, replace) defined by Russel & Burch in 1959. These approaches include in silico methods (databases and computer models), in vitro physicochemical analysis, biological methods using bacteria or isolated cells, reconstructed enzyme systems, and reconstructed tissues. Emerging "omic" methods used in integrated approaches further help to reduce animal use, while stem cells offer promising approaches to toxicologic and pathophysiologic studies, along with organotypic cultures and bio-artificial organs. Only a few alternative methods can so far be used in stand-alone tests as substitutes for animal testing. The best way to use these methods is to integrate them in tiered testing strategies (ITS), in which animals are only used as a last resort. PMID:20669543

  4. Cost-effectiveness analysis of paclitaxel + carboplatin vs. alternative combinations in the treatment of non-small cell lung cancer

    Directory of Open Access Journals (Sweden)

    Mario Eandi

    2006-06-01

    Full Text Available Non-small cell lung cancer (NSCLC is the most common type of lung cancer and its medical and economical burden represents a serious matter in Europe and Usa, due to its high mortality rates and drug costs. Lung cancer is responsible for about 30% of cancer death in men and women; in Europe only about 8 per cent of people with lung cancer survive for 5 years. At present combination chemotherapy based on cisplatin or carboplatin associated with paclitaxel, vinorelbine or gemcitabine is the state of the art for the treatment in patients with stage IIIb or IV NSCLC. Aim of this study was to compare the cost-effectiveness of paclitaxel/carboplatin (PCb, gemcitabine/cisplatin (GC and vinorelbine/cisplatin (VC in the perspective of the Italian National Health Service. Therefore we perfomed a semi-Markov decision model mainly based on clinical results from the Italian Lung Cancer Project. The model included differential direct medical costs registered for two years from starting chemotherapy, using tariffs valid for 2005. Benefits was measured by years of life saved (YOLs. The model also allowed to estimate only costs accrued over the period of time, performing a cost-minimisation analysis. According to cost-effectiveness analysis, VC is dominated because it’s more costly and less effective than GC. On the contrary, combination chemotherapy with GC is more inexpensive but less effective than paclitaxel/carboplatin (PCb: in this case we compared the incremental cost-effectiveness ratio (ICER with a maximum acceptable willingness-to-pay (WTP value. In the base scenario the ICER of PCb over GC treatment is 52,326 euro/ YOLs, which is definitely lower than the maximum acceptable WTP value. Sensitivity analyses confirmed the robustness of the results from cost-effectiveness analysis in the base scenario.

  5. Transport Catastrophe Analysis as an Alternative to a Monofractal Description: Theory and Application to Financial Crisis Time Series

    Directory of Open Access Journals (Sweden)

    Sergey A. Kamenshchikov

    2014-01-01

    Full Text Available The goal of this investigation was to overcome limitations of a persistency analysis, introduced by Benoit Mandelbrot for monofractal Brownian processes: nondifferentiability, Brownian nature of process, and a linear memory measure. We have extended a sense of a Hurst factor by consideration of a phase diffusion power law. It was shown that precatastrophic stabilization as an indicator of bifurcation leads to a new minimum of momentary phase diffusion, while bifurcation causes an increase of the momentary transport. An efficiency of a diffusive analysis has been experimentally compared to the Reynolds stability model application. An extended Reynolds parameter has been introduced as an indicator of phase transition. A combination of diffusive and Reynolds analyses has been applied for a description of a time series of Dow Jones Industrial weekly prices for the world financial crisis of 2007–2009. Diffusive and Reynolds parameters showed extreme values in October 2008 when a mortgage crisis was fixed. A combined R/D description allowed distinguishing of market evolution short-memory and long-memory shifts. It was stated that a systematic large scale failure of a financial system has begun in October 2008 and started fading in February 2009.

  6. Some observations from radiometric ‘8 bit’ data of sediment thin sections based on alternative petrographic image analysis method

    Indian Academy of Sciences (India)

    Sudip Dey; Suvendu Ghosh; Chandrani Debbarma; Prasamita Sarkar; Mhu Aris Marfai; Sabyasachi Maiti

    2009-04-01

    This paper deals with the experiment of sediment microstructure analysis especially microfabric mapping by digital imaging.For that purpose the greyscale images (Red band from RGB combination)of the thin sections have been prepared from the selected 12 samples.The basis of this mapping is the reflectance capacity of different sediments which is influenced by the physical parameters like grain size and colour.The reflectances of different sediments are represented in digital format by different DN values from 0 –255 within the radiometric ranges of ‘8 bit ’data. Density slicing has been chosen as the method of microstructure mapping in this research.This study shows that lower DN values normally present dark coloured coarser sand and clay while higher DN values present light coloured finer sediment samples.In the selected samples for this study the maximum DN value has been found from micaceous materials.Another remarkable thing observed from the microfabric mapping is that the presence of coarser sediments forms complex microfabric pattern than the finer sediments in the study area.Though this method have some demerits,still its simple technique can be very useful for accurate microstructure analysis.

  7. 64-multislice detector computed tomography coronary angiography as potential alternative to conventional coronary angiography: a systematic review and meta-analysis

    DEFF Research Database (Denmark)

    Abdulla, Jawdat; Abildstrøm, Steen Zabell; Gøtzsche, Ole;

    2007-01-01

    AIMS: To evaluate the diagnostic accuracy of 64-slice multi-detector computed tomography coronary angiography (64-SCTA) compared with the standard reference conventional coronary angiography (CCA). METHODS AND RESULTS: Based on a systematic search, 27 studies including 1740 patients were eligible...... coronary artery stenosis in per-segment (19 studies) and per-patients (13 studies) populations were 19 and 57.5% respectively. Accuracy tests with 95% confidence intervals comparing 64-SCTA vs. CCA showed that sensitivity, specificity, positive predictive and negative predictive values for native coronary......% by overall per-segment analysis. CONCLUSION: The high diagnostic accuracy of 64-SCTA validates this non-invasive technique as a potential alternative to CCA in carefully selected populations suspected for coronary stenosis....

  8. An Alternate Approach to Optimal L 2 -Error Analysis of Semidiscrete Galerkin Methods for Linear Parabolic Problems with Nonsmooth Initial Data

    KAUST Repository

    Goswami, Deepjyoti

    2011-09-01

    In this article, we propose and analyze an alternate proof of a priori error estimates for semidiscrete Galerkin approximations to a general second order linear parabolic initial and boundary value problem with rough initial data. Our analysis is based on energy arguments without using parabolic duality. Further, it follows the spirit of the proof technique used for deriving optimal error estimates for finite element approximations to parabolic problems with smooth initial data and hence, it unifies both theories, that is, one for smooth initial data and other for nonsmooth data. Moreover, the proposed technique is also extended to a semidiscrete mixed method for linear parabolic problems. In both cases, optimal L2-error estimates are derived, when the initial data is in L2. A superconvergence phenomenon is also observed, which is then used to prove L∞-estimates for linear parabolic problems defined on two-dimensional spatial domain again with rough initial data. Copyright © Taylor & Francis Group, LLC.

  9. Field measurement of acid gases and soluble anions in atmospheric particulate matter using a parallel plate wet denuder and an alternating filter-based automated analysis system.

    Science.gov (United States)

    Boring, C Bradley; Al-Horr, Rida; Genfa, Zhang; Dasgupta, Pumendu K; Martin, Michael W; Smith, William F

    2002-03-15

    We present a new fully automated instrument for the measurement of acid gases and soluble anionic constituents of atmospheric particulate matter. The instrument operates in two independent parallel channels. In one channel, a wet denuder collects soluble acid gases; these are analyzed by anion chromatography (IC). In a second channel, a cyclone removes large particles and the aerosol stream is then processed by another wet denuder to remove potentially interfering gases. The particles are then collected by one of two glass fiber filters which are alternately sampled, washed, and dried. The washings are preconcentrated and analyzed by IC. Detection limits of low to subnanogram per cubic meter concentrations of most gaseous and particulate constituents can be readily attained. The instrument has been extensively field-tested; some field data are presented. Results of attempts to decipher the total anionic constitution of urban ambient aerosol by IC-MS analysis are also presented.

  10. Numerical analysis of the source-sink alternation of composite global warming potential of the paddy ecosystem in the Yangtze Delta

    Institute of Scientific and Technical Information of China (English)

    刘建栋; 周秀骥; 于强

    2003-01-01

    By coupling the biogeochemical model with plant ecological model, a model was established to reveal the principle of the composite global warming potential transformation in the paddy ecosystem. Validation of the model with the observed data indicated that the model can simulate both the crop growth processes and emissions of CH4 and N2O accurately. Some numerical analyses were made to identify the impacts of different fertilizer application on assimilation of CO2 and emissions of CH4 and N2O, and the transformation principle of the composite global warming potential. Based on the results of the numerical analysis, the source-sink alternation of composite global warming potential in the paddy ecosystem was discovered, and some new conceptions of fertilizer index such as maximum-sink fertilizer, zero-emission fertilizer are put forward in this paper. The fertilizer scheme for Yangtze Delta was proposed to provide the important scientific basis for a sustainable agriculture in this region.

  11. FEM numerical analysis of excimer laser induced modification in alternating multi-layers of amorphous and nano-crystalline silicon films

    Energy Technology Data Exchange (ETDEWEB)

    Conde, J.C., E-mail: jconde@uvigo.es [Dpto. Fisica Aplicada, Universidade de Vigo, Rua Maxwell s/n, Campus Universitario Lagoas Marcosende, Vigo (Spain); Martin, E. [Dpto. Mecanica, Maquinas, Motores Termicos y Fluidos, Universidade de Vigo, Rua Maxwell s/n, Campus Universitario Lagoas Marcosende, Vigo (Spain); Stefanov, S. [Dpto. Fisica Aplicada, Universidade de Vigo, Rua Maxwell s/n, Campus Universitario Lagoas Marcosende, Vigo (Spain); Alpuim, P. [Departamento de Fisica, Universidade do Minho, 4800-058 Guimaraes (Portugal); Chiussi, S. [Dpto. Fisica Aplicada, Universidade de Vigo, Rua Maxwell s/n, Campus Universitario Lagoas Marcosende, Vigo (Spain)

    2012-09-15

    Highlights: Black-Right-Pointing-Pointer nc-Si:H is a material with growing importance for a large-area of nano-electronic, photovoltaic or biomedical devices. Black-Right-Pointing-Pointer UV-ELA technique causes a rapid heating that provokes the H{sub 2} desorption from the Si surface and bulk material. Black-Right-Pointing-Pointer Next, diffusion of P doped nc-Si films and eventually, for high energy densities would be possible to reach the melting point. Black-Right-Pointing-Pointer These multilayer structures consisting of thin alternating a-Si:H(10 nm) and n-doped nc-Si:H(60 nm) films deposited on SiO{sub 2}. Black-Right-Pointing-Pointer To optimize parameters involved in this processing, FEM numerical analysis of multilayer structures have been performed. Black-Right-Pointing-Pointer The numerical results are compared with exhaustive characterization of the experimental results. - Abstract: UV excimer laser annealing (UV-ELA) is an alternative annealing process that, during the last few years, has gained enormous importance for the CMOS nano-electronic technologies, with the ability to provide films and alloys with electrical and optical properties to fit the desired device performance. The UV-ELA of amorphous (a-) and/or doped nano-crystalline (nc-) silicon films is based on the rapid (nanoseconds) formation of temperature profiles caused by laser radiation that is absorbed in the material and lead to crystallisation, diffusion in solid or even in liquid phase. To achieve the desired temperature profiles and to optimize the parameters involved in the processing of hydrogenated nanocrystalline silicon (nc-Si:H) films with the UV-ELA, a numerical analysis by finite element method (FEM) of a multilayer structure has been performed. The multilayer structures, consisting of thin alternating a-Si:H(10 nm) and n-doped nc-Si:H(60 nm) layers, deposited on a glass substrate, has also been experimentally analyzed. Temperature profiles caused by 193 nm radiation with 25

  12. Cost analysis and economic comparison for alternative fuel cycles in the heavy water cooled canadian reactor (CANDU)

    International Nuclear Information System (INIS)

    Three main options in a CANDU fuel cycle involve use of: (1) natural uranium (0.711 weight percent U-235) fuel, (2) slightly enriched uranium (1.2 weight percent U-235) fuel, and (3) recovered uranium (0.83 weight percent U-235) fuel from light water reactor spent fuel. ORIGEN-2 computer code was used to identify composition of the spent fuel for each option, including the standard LWR fuel (3.3 weight percent U-235). Uranium and plutonium credit calculations were performed using ORIGEN-2 output. WIMSD-5 computer code was used to determine maximum discharge burnup values for each case. For the 3 cycles selected (natural uranium, slightly enriched uranium, recovered uranium), levelized fuel cycle cost calculations are performed over the reactor lifetime of 40 years, using unit process costs obtained from literature. Components of the fuel cycle costs are U purchase, conversion, enrichment, fabrication, SF storage, SF disposal, and reprocessing where applicable. Cost parameters whose effects on the fuel cycle cost are to be investigated are escalation ratio, discount rate and SF storage time. Cost estimations were carried out using specially developed computer programs. Share of each cost component on the total cost was determined and sensitivity analysis was performed in order to show how a change in a main cost component affects the fuel cycle cost. The main objective of this study has been to find out the most economical option for CANDU fuel cycle by changing unit prices and cost parameters

  13. Analysis of alternatives for using cable bolts as primary support at two low-seam coal mines

    Institute of Scientific and Technical Information of China (English)

    Esterhuizen Gabriel S; Tulu Ihsan B

    2016-01-01

    Cable bolts are sometimes used in low-seam coal mines to provide support in difficult ground conditions. This paper describes cable bolting solutions at two low-seam coal mines in similar ground conditions. Both mines used support systems incorporating cable bolts as part of the primary support system. Two original cable bolt based support systems as well as two modified systems are evaluated to estimate their ability to prevent large roof falls. One of the support systems incorporated passive cable bolts, while the other used pre-tensioned cable bolts. The results and experience at the mines showed that the mod-ified systems provided improved stability over the original support systems. The presence of the cable bolts is the most important contribution to stability against large roof falls, rather than the details of the support pattern. It was also found that a heavy steel channel can improve the safety of the system because of the‘sling’ action it provides. Additionally, the analysis showed that fully-grouted rebar bolts load much earlier than the cable bolts, and pre-tensioning of the cable bolts can result in a more uniform distribution of loading in the roof.

  14. Thermodynamic Analysis of Actual Vapour Compression System with R12 and Its Eco-Friendly Alternatives Refrigerants

    Directory of Open Access Journals (Sweden)

    Vijay Singh Bisht

    2014-04-01

    Full Text Available This paper presents a theoretical performance study of a vapour compression refrigeration system with refrigerants R-12, R134a and R1234yf. A computational model based on energy first law analysis is presented for the investigation of the effects of evaporating temperatures, degree of subcooling, dead state temperatures and effectiveness of the liquid vapour heat exchanger on the relative capacity change index, coefficient of performance of the vapour compression refrigeration cycle. RCI (relative capacity change index of the system is highest for R1234yf and with increase in degree of subcooling; R1234yf has the highest percentage increase in COP. The total compressor work requirement for system is highest with R134a. R1234yf is the only refrigerants of all the refrigerants used in present work that satisfy MAC directive (2006/40/EG because of GWP value less than 150. From these results, it is indicated that R1234yf is the refrigerant for future.

  15. lociNGS: a lightweight alternative for assessing suitability of next-generation loci for evolutionary analysis.

    Directory of Open Access Journals (Sweden)

    Sarah M Hird

    Full Text Available Genomic enrichment methods and next-generation sequencing produce uneven coverage for the portions of the genome (the loci they target; this information is essential for ascertaining the suitability of each locus for further analysis. lociNGS is a user-friendly accessory program that takes multi-FASTA formatted loci, next-generation sequence alignments and demographic data as input and collates, displays and outputs information about the data. Summary information includes the parameters coverage per locus, coverage per individual and number of polymorphic sites, among others. The program can output the raw sequences used to call loci from next-generation sequencing data. lociNGS also reformats subsets of loci in three commonly used formats for multi-locus phylogeographic and population genetics analyses - NEXUS, IMa2 and Migrate. lociNGS is available at https://github.com/SHird/lociNGS and is dependent on installation of MongoDB (freely available at http://www.mongodb.org/downloads. lociNGS is written in Python and is supported on MacOSX and Unix; it is distributed under a GNU General Public License.

  16. Alternative CHCA-based matrices for the analysis of low molecular weight compounds by UV-MALDI-tandem mass spectrometry.

    Science.gov (United States)

    Porta, Tiffany; Grivet, Chantal; Knochenmuss, Richard; Varesio, Emmanuel; Hopfgartner, Gérard

    2011-02-01

    Analysis of low molecular weight compounds (LMWC) in complex matrices by vacuum matrix-assisted laser desorption/ionization (MALDI) often suffers from matrix interferences, which can severely degrade limits of quantitation. It is, therefore, useful to have available a range of suitable matrices, which exhibit complementary regions of interference. Two newly synthesized α-cyanocinnamic acid derivatives are reported here; (E)-2-cyano-3-(naphthalen-2-yl)acrylic acid (NpCCA) and (2E)-3-(anthracen-9-yl)-2-cyanoprop-2enoic acid (AnCCA). Along with the commonly used α-cyano-4-hydroxycinnamic acid (CHCA), and the recently developed 4-chloro-α-cyanocinnamic acid (Cl-CCA) matrices, these constitute a chemically similar series of matrices covering a range of molecular weights, and with correspondingly differing ranges of spectral interference. Their performance was compared by measuring the signal-to-noise ratios (S/N) of 47 analytes, mostly pharmaceuticals, with the different matrices using the selected reaction monitoring (SRM) mode on a triple quadrupole instrument equipped with a vacuum MALDI source. AnCCA, NpCCA and Cl-CCA were found to offer better signal-to-noise ratios in SRM mode than CHCA, but Cl-CCA yielded the best results for 60% of the compounds tested. To better understand the relative performance of this matrix series, the proton affinities (PAs) were measured using the kinetic method. Their relative values were: AnCCA > CHCA > NpCCA > Cl-CCA. This ordering is consistent with the performance data. The synthesis of the new matrices is straightforward and they provide (1) tunability of matrix background interfering ions and (2) enhanced analyte response for certain classes of compounds. PMID:21259393

  17. State alternative route designations

    Energy Technology Data Exchange (ETDEWEB)

    1989-07-01

    Pursuant to the Hazardous Materials Transportation Act (HMTA), the Department of Transportation (DOT) has promulgated a comprehensive set of regulations regarding the highway transportation of high-level radioactive materials. These regulations, under HM-164 and HM-164A, establish interstate highways as the preferred routes for the transportation of radioactive materials within and through the states. The regulations also provide a methodology by which a state may select alternative routes. First,the state must establish a ``state routing agency,`` defined as an entity authorized to use the state legal process to impose routing requirements on carriers of radioactive material (49 CFR 171.8). Once identified, the state routing agency must select routes in accordance with Large Quantity Shipments of Radioactive Materials or an equivalent routing analysis. Adjoining states and localities should be consulted on the impact of proposed alternative routes as a prerequisite of final route selection. Lastly, the states must provide written notice of DOT of any alternative route designation before the routes are deemed effective.

  18. State alternative route designations

    Energy Technology Data Exchange (ETDEWEB)

    1989-07-01

    Pursuant to the Hazardous Materials Transportation Act (HMTA), the Department of Transportation (DOT) has promulgated a comprehensive set of regulations regarding the highway transportation of high-level radioactive materials. These regulations, under HM-164 and HM-164A, establish interstate highways as the preferred routes for the transportation of radioactive materials within and through the states. The regulations also provide a methodology by which a state may select alternative routes. First,the state must establish a state routing agency,'' defined as an entity authorized to use the state legal process to impose routing requirements on carriers of radioactive material (49 CFR 171.8). Once identified, the state routing agency must select routes in accordance with Large Quantity Shipments of Radioactive Materials or an equivalent routing analysis. Adjoining states and localities should be consulted on the impact of proposed alternative routes as a prerequisite of final route selection. Lastly, the states must provide written notice of DOT of any alternative route designation before the routes are deemed effective.

  19. Alternative fuels for vehicles; Alternative drivmidler

    Energy Technology Data Exchange (ETDEWEB)

    2012-02-15

    Up until 2020 and onwards the analysis indicates that especially electricity, biogas and natural gas as propellants is economically attractive compared to conventional gasoline and diesel while other fuels have the same or higher costs for petrol and diesel. Especially biogas and electricity will also offer significant reductions in CO{sub 2} emissions, but also hydrogen, methanol, DME and to a lesser extent the second generation bioethanol and most of the other alternative fuels reduce CO{sub 2} emissions. Use of the traditional food-based first generation biofuels involves, at best, only modest climate benefits if land use changes are counted, and at worst, significant negative climate effects. Natural gas as a propellant involves a moderate climate gain, but may play a role for building infrastructure and market for gaseous fuels in large fleets, thereby contributing to the phasing in of biogas for transport. The electric-based automotive fuels are the most effective due to a high efficiency of the engine and an increasing proportion of wind energy in the electricity supply. The methanol track also has a relatively high efficiency. Among the others, the track based on diesel engines (biodiesel) is more effective than the track based on gasoline/Otto engines (gas and ethanol) as a result of the diesel engine's better efficiency. For the heavy vehicles all the selected alternative fuels to varying degrees reduce emissions of CO{sub 2}, particularly DME based on wood. The only exception to this is - as for passenger cars - the propellant synthetic diesel based on coal. (LN).

  20. Identification and evolutionary analysis of novel exons and alternative splicing events using cross-species EST-to-genome comparisons in human, mouse and rat

    Directory of Open Access Journals (Sweden)

    Ho Jar-Yi

    2006-03-01

    Full Text Available Abstract Background Alternative splicing (AS is important for evolution and major biological functions in complex organisms. However, the extent of AS in mammals other than human and mouse is largely unknown, making it difficult to study AS evolution in mammals and its biomedical implications. Results Here we describe a cross-species EST-to-genome comparison algorithm (ENACE that can identify novel exons for EST-scanty species and distinguish conserved and lineage-specific exons. The identified exons represent not only novel exons but also evolutionarily meaningful AS events that are not previously annotated. A genome-wide AS analysis in human, mouse and rat using ENACE reveals a total of 758 novel cassette-on exons and 167 novel retained introns that have no EST evidence from the same species. RT-PCR-sequencing experiments validated ~50 ~80% of the tested exons, indicating high presence of exons predicted by ENACE. ENACE is particularly powerful when applied to closely related species. In addition, our analysis shows that the ENACE-identified AS exons tend not to pass the nonsynonymous-to-synonymous substitution ratio test and not to contain protein domain, implying that such exons may be under positive selection or relaxed negative selection. These AS exons may contribute to considerable inter-species functional divergence. Our analysis further indicates that a large number of exons may have been gained or lost during mammalian evolution. Moreover, a functional analysis shows that inter-species divergence of AS events may be substantial in protein carriers and receptor proteins in mammals. These exons may be of interest to studies of AS evolution. The ENACE programs and sequences of the ENACE-identified AS events are available for download. Conclusion ENACE can identify potential novel cassette exons and retained introns between closely related species using a comparative approach. It can also provide information regarding lineage- or species

  1. Life-Cycle Cost and Risk Analysis of Alternative Configurations for Shipping Low-Level Radioactive Waste to the Nevada Test Site

    Energy Technology Data Exchange (ETDEWEB)

    PM Daling; SB Ross; BM Biwer

    1999-12-17

    The Nevada Test Site (NTS) is a major receiver of low-level radioactive waste (LLW) for disposal. Currently, all LLW received at NTS is shipped by truck. The trucks use highway routes to NTS that pass through the Las Vegas Valley and over Hoover Dam, which is a concern of local stakeholder groups in the State of Nevada. Rail service offers the opportunity to reduce transportation risks and costs, according to the Waste Management Programmatic Environmental Impact Statement (WM-PEIS). However, NTS and some DOE LLW generator sites are not served with direct rail service so intermodal transport is under consideration. Intermodal transport involves transport via two modes, in this case truck and rail, from the generator sites to NTS. LLW shipping containers would be transferred between trucks and railcars at intermodal transfer points near the LLW generator sites, NTS, or both. An Environmental Assessment (EA)for Intermodal Transportation of Low-Level Radioactive Waste to the Nevada Test Site (referred to as the NTSIntermodal -M) has been prepared to determine whether there are environmental impacts to alterations to the current truck routing or use of intermodal facilities within the State of Nevada. However, an analysis of the potential impacts outside the State of Nevada are not addressed in the NTS Intermodal EA. This study examines the rest of the transportation network between LLW generator sites and the NTS and evaluates the costs, risks, and feasibility of integrating intermodal shipments into the LLW transportation system. This study evaluates alternative transportation system configurations for NTS approved and potential generators based on complex-wide LLW load information. Technical judgments relative to the availability of DOE LLW generators to ship from their sites by rail were developed. Public and worker risk and life-cycle cost components are quantified. The study identifies and evaluates alternative scenarios that increase the use of rail (intermodal

  2. Life-Cycle Cost and Risk Analysis of Alternative Configurations for Shipping Low-Level Radioactive Waste to the Nevada Test Site

    International Nuclear Information System (INIS)

    The Nevada Test Site (NTS) is a major receiver of low-level radioactive waste (LLW) for disposal. Currently, all LLW received at NTS is shipped by truck. The trucks use highway routes to NTS that pass through the Las Vegas Valley and over Hoover Dam, which is a concern of local stakeholder groups in the State of Nevada. Rail service offers the opportunity to reduce transportation risks and costs, according to the Waste Management Programmatic Environmental Impact Statement (WM-PEIS). However, NTS and some DOE LLW generator sites are not served with direct rail service so intermodal transport is under consideration. Intermodal transport involves transport via two modes, in this case truck and rail, from the generator sites to NTS. LLW shipping containers would be transferred between trucks and railcars at intermodal transfer points near the LLW generator sites, NTS, or both. An Environmental Assessment (EA)for Intermodal Transportation of Low-Level Radioactive Waste to the Nevada Test Site (referred to as the NTSIntermodal -M) has been prepared to determine whether there are environmental impacts to alterations to the current truck routing or use of intermodal facilities within the State of Nevada. However, an analysis of the potential impacts outside the State of Nevada are not addressed in the NTS Intermodal EA. This study examines the rest of the transportation network between LLW generator sites and the NTS and evaluates the costs, risks, and feasibility of integrating intermodal shipments into the LLW transportation system. This study evaluates alternative transportation system configurations for NTS approved and potential generators based on complex-wide LLW load information. Technical judgments relative to the availability of DOE LLW generators to ship from their sites by rail were developed. Public and worker risk and life-cycle cost components are quantified. The study identifies and evaluates alternative scenarios that increase the use of rail (intermodal

  3. (1)H NMR metabolomic study of auxotrophic starvation in yeast using Multivariate Curve Resolution-Alternating Least Squares for Pathway Analysis.

    Science.gov (United States)

    Puig-Castellví, Francesc; Alfonso, Ignacio; Piña, Benjamin; Tauler, Romà

    2016-01-01

    Disruption of specific metabolic pathways constitutes the mode of action of many known toxicants and it is responsible for the adverse phenotypes associated to human genetic defects. Conversely, many industrial applications rely on metabolic alterations of diverse microorganisms, whereas many therapeutic drugs aim to selectively disrupt pathogens' metabolism. In this work we analyzed metabolic changes induced by auxotrophic starvation conditions in yeast in a non-targeted approach, using one-dimensional proton Nuclear Magnetic Resonance spectroscopy ((1)H NMR) and chemometric analyses. Analysis of the raw spectral datasets showed specific changes linked to the different stages during unrestricted yeast growth, as well as specific changes linked to each of the four tested starvation conditions (L-methionine, L-histidine, L-leucine and uracil). Analysis of changes in concentrations of more than 40 metabolites by Multivariate Curve Resolution - Alternating Least Squares (MCR-ALS) showed the normal progression of key metabolites during lag, exponential and stationary unrestricted growth phases, while reflecting the metabolic blockage induced by the starvation conditions. In this case, different metabolic intermediates accumulated over time, allowing identification of the different metabolic pathways specifically affected by each gene disruption. This synergy between NMR metabolomics and molecular biology may have clear implications for both genetic diagnostics and drug development. PMID:27485935

  4. Flow cytometry and adenosine tri-phosphate analysis: alternative possibilities to evaluate major bacteriological changes in drinking water treatment and distribution systems.

    Science.gov (United States)

    Vital, Marius; Dignum, Marco; Magic-Knezev, Aleksandra; Ross, Petra; Rietveld, Luuk; Hammes, Frederik

    2012-10-01

    An ever-growing need exists for rapid, quantitative and meaningful methods to quantify and characterize the effect of different treatment steps on the microbiological processes and events that occur during drinking water treatment and distribution. Here we compared cultivation-independent flow cytometry (FCM) and adenosine tri-phosphate (ATP) analysis with conventional cultivation-based microbiological methods, on water samples from two full-scale treatment and distribution systems. The two systems consist of nearly identical treatment trains, but their raw water quality and pre-treatment differed significantly. All of the drinking water treatment processes affected the microbiological content of the water considerably, but once treated, the finished water remained remarkably stable throughout the distribution system. Both the FCM and ATP data were able to describe the microbiology of the systems accurately, providing meaningful process data when combined with other parameters such as dissolved organic carbon analysis. Importantly, the results highlighted a complimentary value of the two independent methods: while similar trends were mostly observed, variations in ATP-per-cell values between water samples were adequately explained by differences in the FCM fingerprints of the samples. This work demonstrates the value of alternative microbial methods for process/system control, optimization and routine monitoring of the general microbial quality of water during treatment and distribution. PMID:22763289

  5. 1H NMR metabolomic study of auxotrophic starvation in yeast using Multivariate Curve Resolution-Alternating Least Squares for Pathway Analysis

    Science.gov (United States)

    Puig-Castellví, Francesc; Alfonso, Ignacio; Piña, Benjamin; Tauler, Romà

    2016-01-01

    Disruption of specific metabolic pathways constitutes the mode of action of many known toxicants and it is responsible for the adverse phenotypes associated to human genetic defects. Conversely, many industrial applications rely on metabolic alterations of diverse microorganisms, whereas many therapeutic drugs aim to selectively disrupt pathogens’ metabolism. In this work we analyzed metabolic changes induced by auxotrophic starvation conditions in yeast in a non-targeted approach, using one-dimensional proton Nuclear Magnetic Resonance spectroscopy (1H NMR) and chemometric analyses. Analysis of the raw spectral datasets showed specific changes linked to the different stages during unrestricted yeast growth, as well as specific changes linked to each of the four tested starvation conditions (L-methionine, L-histidine, L-leucine and uracil). Analysis of changes in concentrations of more than 40 metabolites by Multivariate Curve Resolution – Alternating Least Squares (MCR-ALS) showed the normal progression of key metabolites during lag, exponential and stationary unrestricted growth phases, while reflecting the metabolic blockage induced by the starvation conditions. In this case, different metabolic intermediates accumulated over time, allowing identification of the different metabolic pathways specifically affected by each gene disruption. This synergy between NMR metabolomics and molecular biology may have clear implications for both genetic diagnostics and drug development. PMID:27485935

  6. Space shuttle/food system. Volume 2, Appendix C: Food cooling techniques analysis. Appendix D: Package and stowage: Alternate concepts analysis

    Science.gov (United States)

    1974-01-01

    The relative penalties associated with various techniques for providing an onboard cold environment for storage of perishable food items, and for the development of packaging and vehicle stowage parameters were investigated in terms of the overall food system design analysis of space shuttle. The degrees of capability for maintaining both a 40 F to 45 F refrigerated temperature and a 0 F and 20 F frozen environment were assessed for the following cooling techniques: (1) phase change (heat sink) concept; (2) thermoelectric concept; (3) vapor cycle concept; and (4) expendable ammonia concept. The parameters considered in the analysis were weight, volume, and spacecraft power restrictions. Data were also produced for packaging and vehicle stowage parameters which are compatible with vehicle weight and volume specifications. Certain assumptions were made for food packaging sizes based on previously generated space shuttle menus. The results of the study are shown, along with the range of meal choices considered.

  7. Matrix-free analysis of selected benzodiazepines in human serum samples using alternating trilinear decomposition modeling of fast liquid chromatography diode array detection data.

    Science.gov (United States)

    Vosough, Maryam; Iravani, Negar J

    2016-02-01

    This paper describes the development and validation of a simple and efficient bioanalytical procedure for simultaneous determination of alprazolam, clonazepam, diazepam in human serum samples using high performance liquid chromatography with photodiode-array detection, regarding a fast elution methodology in 4 min. Briefly, this method consists of a simple liquid extraction step of serum samples followed by HPLC analysis on a C18 column. After confirming the absence of matrix effect, an external standard methodology has been applied for quantification purposes. Due to the presence of serum endogenous components as uncalibrated components in the sample, the second-order calibration based on alternating trilinear decomposition has been applied on a set of absorbance matrices collected as a function of retention time and wavelengths. Acceptable resolution and quantification results were achieved in the presence of matrix interferences and the second-order advantage was fully exploited. The average recoveries for alprazolam, clonazepam and diazepam were 89.1%, 96.3% and 94.7% and relative standard deviation values for intra- and inter-day precision were equal or lower than 8.1% and 9.4%, respectively. The developed method enabled us to determine the analytes in the various serum samples in the presence of overlapped profiles, while keeping experimental time and extraction step at the minimum. PMID:26653472

  8. Outcomes analysis of an alternative formulation of PEGylated liposomal doxorubicin in recurrent epithelial ovarian carcinoma during the drug shortage era [Corrigendum

    Directory of Open Access Journals (Sweden)

    Berger JL

    2015-03-01

    Full Text Available Berger JL, Smith A, Zorn KK, et al. Onco Targets Ther. 2014;7:1409–1413.It has been brought to our attention that there are two pharmaceutical companies that market drugs with the name ‘lipodox’ and we erroneously refer to the product used in our current study-Outcomes analysis of an alternative formulation of PEGylated liposomal doxorubicin in recurrent epithelial ovarian carcinoma during the drug shortage era. by the trade name Lipo-dox®, a drug produced by TTY Biopharm Co. Ltd, when the Sun Pharma product is Lipodox. Lipo-Dox® utilizes a different liposomal particle than Doxil®, DSCP versus HSCP. Due to the similarity of the drug names, we were under the mistaken impression that Sun Pharma’s Lipodox utilized the DSCP liposome. We postulated that this might be related to the poor clinical outcomes seen in our population. It is now clear that Lipodox utilizes the same liposome as Doxil® and this is not a relevant point of discussion. The authors would like to let the readers know that Lipodox uses HSCP as the liposome and this should be corrected in the manuscript. The patients in our current study were all treated with Lipodox, and this drug uses HSCP as the liposome.

  9. Identification and functional analysis of porcine basic helix-loop-helix transcriptional factor 3 (TCF3) and its alternative splicing isoforms.

    Science.gov (United States)

    Yang, Fan; Wang, Ning; Liu, Yajun; Wang, Huayan

    2016-04-01

    The transcription factor 3 (TCF3) is a basic helix-loop-helix transcription factor and is essential for lymphocyte development and epithelial-mesenchymal transition. The splicing isoform, genomic organization and physiological roles of TCF3 have not been elucidated well in pig. Based on RNA-seq database, four alternative splicing isoforms were identified. Splicing isoforms TCF3(E12), TCF3(E47), and TCF3A expressed globally in porcine tissues, but TCF3B mainly expressed in spleen and endoderm derived tissues, such as pancreas and lung. The functional analysis showed that TCF3(E12), TCF3(E47), and TCF3B were translocated exclusively into nuclei, yet TCF3A was distributed in cytoplasm. The investigation of clinical specimens showed that TCF3 expression was significantly reduced in spleen tissues that were infected by classical swine fever virus (CSFV). This study is for the first time to report two novel splicing isoforms TCF3A and TCF3B, which may play an important role in lymphocyte maturation and have the correlation with CSFV evasion. PMID:27033898

  10. Ethyl-bridged hybrid column as an efficient alternative for HPLC analysis of plasma amino acids by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate.

    Science.gov (United States)

    Castellanos, Mar; Van Eendenburg, Cecile Van; Gubern, Carme; Sanchez, Juan M

    2016-09-01

    Conventional C18 silica columns have proven to be useful for the analysis of amino acids (AA) from protein hydrolysates but undesirable peak overlapping is usually found when analyzing body fluids given that a large number of AAs are present in the samples. As an alternative to silica packings, an ethyl-bridged packing for reversed-phase liquid chromatography of derivatized AAs with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) has been evaluated. The new packing material improves the separation efficiency allowing better separations when analyzing biological fluids. Moreover, this packing has advantages for routine AA analysis, such as a decrease in the total running time and an increase in the life-time of the columns. The pH of the mobile phase has a significant effect on the elution behavior of the AQC hydrolysis product (AMQ) and on the AA derivatives. It is not possible to elute AMQ before detecting the first AA derivative, which requires an accurate adjustment of the pH in the range of 5.30-5.35 to obtain good separation and resolution for the most polar compounds. Under the conditions proposed, it is possible to separate all AAs except the Gly-Gln pair, which is not a problem when hydrolyzed samples are analyzed. The AMQ-Ser pair requires either the use of a different mobile phase pH for its baseline separation or the use of fluorescence detection. Two different procedures for protein removal from plasma samples have been evaluated, solvent precipitation and ultrafiltration (UF) and it has been found that UF gives better results as no significant losses of AAs were observed. The validation of the proposed method with UV detection gives method detection limits in the range of 8-12μM, with repeatability values<8% (n=6) and inter-day precision in plasma samples ranging from 4 to 13% (n=4). PMID:27428457

  11. Ethyl-bridged hybrid column as an efficient alternative for HPLC analysis of plasma amino acids by pre-column derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate.

    Science.gov (United States)

    Castellanos, Mar; Van Eendenburg, Cecile Van; Gubern, Carme; Sanchez, Juan M

    2016-09-01

    Conventional C18 silica columns have proven to be useful for the analysis of amino acids (AA) from protein hydrolysates but undesirable peak overlapping is usually found when analyzing body fluids given that a large number of AAs are present in the samples. As an alternative to silica packings, an ethyl-bridged packing for reversed-phase liquid chromatography of derivatized AAs with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) has been evaluated. The new packing material improves the separation efficiency allowing better separations when analyzing biological fluids. Moreover, this packing has advantages for routine AA analysis, such as a decrease in the total running time and an increase in the life-time of the columns. The pH of the mobile phase has a significant effect on the elution behavior of the AQC hydrolysis product (AMQ) and on the AA derivatives. It is not possible to elute AMQ before detecting the first AA derivative, which requires an accurate adjustment of the pH in the range of 5.30-5.35 to obtain good separation and resolution for the most polar compounds. Under the conditions proposed, it is possible to separate all AAs except the Gly-Gln pair, which is not a problem when hydrolyzed samples are analyzed. The AMQ-Ser pair requires either the use of a different mobile phase pH for its baseline separation or the use of fluorescence detection. Two different procedures for protein removal from plasma samples have been evaluated, solvent precipitation and ultrafiltration (UF) and it has been found that UF gives better results as no significant losses of AAs were observed. The validation of the proposed method with UV detection gives method detection limits in the range of 8-12μM, with repeatability values<8% (n=6) and inter-day precision in plasma samples ranging from 4 to 13% (n=4).

  12. Multi-batch catfish production and economic analysis using alternative low-cost diets with corn gluten feed and traditional diets with meat-and-bone meal

    Science.gov (United States)

    We conducted concurrent feeding trials for one growing season with channel catfish in ponds in Mississippi and Arkansas to evaluate the production and economic effects of alternative (low-cost) feeds containing 28 or 32% protein and alternative (corn gluten feed) or traditional (porcine meat, bone a...

  13. Deep sequence analysis of non-small cell lung cancer: Integrated analysis of gene expression, alternative splicing, and single nucleotide variations in lung adenocarcinomas with and without oncogenic KRAS mutations

    Directory of Open Access Journals (Sweden)

    Krishna R Kalari

    2012-02-01

    Full Text Available KRAS mutations are highly prevalent in non-small cell lung cancer (NSCLC, and tumors harboring these mutations tend to be aggressive and resistant to chemotherapy. We used next-generation sequencing technology to identify pathways that are specifically altered in lung tumors harboring a KRAS mutation. Paired-end RNA-sequencing of 15 primary lung adenocarcinoma tumors (8 harboring mutant KRAS and 7 with wild-type KRAS were performed. Sequences were mapped to the human genome, and genomic features, including differentially expressed genes, alternate splicing isoforms and single nucleotide variants, were determined for tumors with and without KRAS mutation using a variety of computational methods. Network analysis was carried out on genes showing differential expression (374 genes, alternate splicing (259 genes and SNV-related changes (65 genes in NSCLC tumors harboring a KRAS mutation. Genes exhibiting two or more connections from the lung adenocarcinoma network were used to carry out integrated pathway analysis. The most significant signaling pathways identified through this analysis were the NFkB, ERK1/2 and AKT pathways. A 27 gene mutant KRAS-specific sub network was extracted based on gene-gene connections within the integrated network, and interrogated for druggable targets. Our results confirm previous evidence that mutant KRAS tumors exhibit activated NFkB, ERK1/2 and AKT pathways and may be preferentially sensitive to target therapeutics toward these pathways. In addition, our analysis indicates novel, previously unappreciated links between mutant KRAS and the TNFR and PPARγ signaling pathways, suggesting that targeted PPARγ antagonists and TNFR inhibitors may be useful therapeutic strategies for treatment of mutant KRAS lung tumors. Our study is the first to integrate genomic features from RNA-Seq data from NSCLC and to define a first draft genomic landscape model that is unique to tumors with oncogenic KRAS mutations.

  14. Assessment of costs and benefits of flexible and alternative fuel use in the U.S. transportation sector. Technical report fourteen: Market potential and impacts of alternative fuel use in light-duty vehicles -- A 2000/2010 analysis

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-01-01

    In this report, estimates are provided of the potential, by 2010, to displace conventional light-duty vehicle motor fuels with alternative fuels--compressed natural gas (CNG), liquefied petroleum gas (LPG), methanol from natural gas, ethanol from grain and from cellulosic feedstocks, and electricity--and with replacement fuels such as oxygenates added to gasoline. The 2010 estimates include the motor fuel displacement resulting both from government programs (including the Clean Air Act and EPACT) and from potential market forces. This report also provides an estimate of motor fuel displacement by replacement and alterative fuels in the year 2000. However, in contrast to the 2010 estimates, the year 2000 estimate is restricted to an accounting of the effects of existing programs and regulations. 27 figs., 108 tabs.

  15. Complementary and Alternative Medicine

    Science.gov (United States)

    ... Help a Friend Who Cuts? Complementary and Alternative Medicine KidsHealth > For Teens > Complementary and Alternative Medicine Print ... replacement. continue How Is CAM Different From Conventional Medicine? Conventional medicine is based on scientific knowledge of ...

  16. Alternative medicine - pain relief

    Science.gov (United States)

    Alternative medicine refers to treatments that are used instead of conventional (standard) ones. If you use an alternative ... considered complementary therapy. There are many forms of ... Acupuncture involves stimulating certain acupoints on the body ...

  17. Multi-walled carbon nanotubes as alternative reversed-dispersive solid phase extraction materials in pesticide multi-residue analysis with QuEChERS method.

    Science.gov (United States)

    Zhao, Pengyue; Wang, Lei; Zhou, Li; Zhang, Fengzu; Kang, Shu; Pan, Canping

    2012-02-17

    A multi-residue method based on modified QuEChERS sample preparation with multi-walled carbon nanotubes (MWCNTs) as reversed-dispersive solid phase extraction (r-DSPE) material and gas chromatography-mass spectrometry determination by selected ion monitoring (GC/MS-SIM) mode was validated on 30 representative pesticides residues in vegetables and fruits. The acetonitrile-based QuEChERS (quick, easy, cheap, effective, rugged and safe) sample preparation technique was used to obtain the extracts, and the further cleanup was carried out by applying r-DSPE. It was found that the amount of MWCNTs influenced the cleanup performance and the recoveries. The optimal amount of 10mg MWCNTs was suitable for cleaning up all selected matrices, as a suitable alternative r-DSPE material to primary secondary amine (PSA). This method was validated on cabbage, spinach, grape and orange spiked at concentration levels of 0.02 and 0.2 mg/kg. The recoveries of 30 pesticides were in the range of 71-110%, with relative standard deviations (RSDs, n=5) lower than 15%. Matrix effects were observed by comparing the slope of matrix-matched standard calibration with that of solvent. Good linearity was achieved at the concentration levels of 0.02-0.5 mg/L. The limits of quantification (LOQs) and the limits of detection (LODs) for 30 pesticides ranged from 0.003 to 0.05 mg/kg and 0.001 to 0.02 mg/kg at the signal-to-noise ratio (S/N) of 10 and 3, respectively. The method was successfully applied to analysis real samples in Beijing. In conclusion, the modified QuEChERS method with MWCNTs cleanup step showed reliable method validation performances and good cleanup effects in this study. PMID:22227363

  18. Cost-effectiveness analysis of the direct and indirect impact of intranasal live attenuated influenza vaccination strategies in children: alternative country profiles

    Science.gov (United States)

    Gibson, Edward; Begum, Najida; Martinón-Torres, Federico; Safadi, Marco Aurélio; Sackeyfio, Alfred; Hackett, Judith; Rajaram, Sankarasubramanian

    2016-01-01

    Background Influenza poses a significant burden on healthcare systems and society, with under-recognition in the paediatric population. Existing vaccination policies (largely) target the elderly and other risk groups where complications may arise. Objective The goal of this study was to evaluate the cost-effectiveness of annual paediatric vaccination (in 2–17-year-olds) with live attenuated influenza vaccination (LAIV), as well as the protective effect on the wider population in England and Wales (base). The study aimed to demonstrate broad applications of the model in countries where epidemiological and transmission data is limited and that have sophisticated vaccination policies (Brazil, Spain, and Taiwan). Methods The direct and indirect impact of LAIV in the paediatric cohort was simulated using an age-stratified dynamic transmission model over a 5-year time horizon of daily cycles and applying discounting of 3.5% in the base case. Pre-existing immunity structure was based on a 1-year model run. Sensitivity analyses were conducted. Results In the base case for England and Wales, the annual paediatric strategy with LAIV was associated with improvements in influenza-related events and quality-adjusted life years (QALYs) lost, yielding an incremental cost per QALY of £6,208. The model was robust to change in the key input parameters. The probabilistic analysis demonstrated LAIV to be cost effective in more than 99% of iterations, assuming a willingness-to-pay threshold of £30,000. Incremental costs per QALY for Brazil were £2,817, and for the cases of Spain and Taiwan the proposed strategy was dominant over the current practice. Conclusion In addition to existing policies, annual paediatric vaccination using LAIV provides a cost-effective strategy that offers direct and indirect protection in the wider community. Paediatric vaccination strategies using LAIV demonstrated clinical and economic benefits over alternative (current vaccination) strategies in

  19. Sequence Analysis and Potentials of the Native RbcS Promoter in the Development of an Alternative Eukaryotic Expression System Using Green Microalga Ankistrodesmus convolutus

    Directory of Open Access Journals (Sweden)

    Suhaimi Napis

    2012-02-01

    Full Text Available The availability of highly active homologous promoters is critical in the development of a transformation system and improvement of the transformation efficiency. To facilitate transformation of green microalga Ankistrodesmus convolutus which is considered as a potential candidate for many biotechnological applications, a highly-expressed native promoter sequence of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (AcRbcS has been used to drive the expression of β-glucuronidase (gusA gene in this microalga. Besides the determination of the transcription start site by 5¢-RACE, sequence analysis revealed that AcRbcS promoter contained consensus TATA-box and several putative cis-acting elements, including some representative light-regulatory elements (e.g., G-box, Sp1 motif and SORLIP2, which confer light responsiveness in plants, and several potential conserved motifs (e.g., CAGAC-motif, YCCYTGG-motifs and CACCACA-motif, which may be involved in light responsiveness of RbcS gene in green microalgae. Using AcRbcS promoter::gusA translational fusion, it was demonstrated that this promoter could function as a light-regulated promoter in transgenic A. convolutus, which suggested that the isolated AcRbcS promoter was a full and active promoter sequence that contained all cis-elements required for developmental and light-mediated control of gene expression, and this promoter can be used to drive the expression of heterologous genes in A. convolutus. This achievement therefore advances the development of A. convolutus as an alternative expression system for the production of recombinant proteins. This is the first report on development of gene manipulation system for unicellular green alga A. convolutus.

  20. A meta-analysis comparing the safety and efficacy of azithromycin over the alternate drugs used for treatment of uncomplicated enteric fever

    Directory of Open Access Journals (Sweden)

    N A Trivedi

    2012-01-01

    Full Text Available Background: Drug-resistant typhoid fever is a major clinical problem globally. Emergence of multidrug-resistant (MDR S. Typhi has complicated therapy by limiting treatment options. Objectives: A meta-analysis was planned to determine the strength of evidence supporting use of azithromycin over the alternate drugs available for treatment of uncomplicated typhoid fever. Materials and Methods: Studies were identified using electronic database such as MEDLINE and other data at the National Library of Medicine assessed using PUBMED search engine as well as Cochrane Clinical Trial Register. Randomized control trials (RCTs comparing azithromycin with chloramphenicol, fluoroquinolones and cephalosporins in culture-proven enteric fever were included. Data was extracted and methodological quality was assessed. Risk ratio (RR with 95% confidence intervals was estimated for the dichotomous outcomes and mean difference (MD with 95% confidence was estimated for continuous data. Primary outcomes studied were clinical failure (CF, microbiological failure, and relapse. Results: A total of seven RCTs involving 773 patients met with our inclusion criteria. In comparison to older fluoroquinolones, azithromycin is marginally better in reducing the chance of CF with RR 0.46 (95% CI 0.25-0.82, while in comparison to ceftriaxone, it significantly reduced the chance of relapse with RR 0.1 (95% CI 0.01- 0.76. There were no serious adverse events reported in any of the trials. Conclusion: Azithromycin can be recommended as a second-line drug in MDR typhoid fever, however, large trials involving pediatric age group patients are recommended to arrive at a definite conclusion.

  1. Therapeutic efficacy of alternative primaquine regimens to standard treatment in preventing relapses by Plasmodium vivax: A systematic review and meta-analysis

    Directory of Open Access Journals (Sweden)

    Lina Marcela Zuluaga-Idarraga

    2015-12-01

    Full Text Available Objective:To compare efficacy and safety of primaquine regimens currently used to prevent relapses by P. vivax.Methods:A systematic review was carried out to identify clinical trials evaluating efficacy and safety to prevent malaria recurrences by P. vivax of primaquine regimen 0.5 mg / kg / day for 7 or 14 days compared to standard regimen of 0.25 mg/kg/day for 14 days. Efficacy of primaquine according to cumulative incidence of recurrences after 28 days was determined. The overall relative risk with fixed-effects meta-analysis was estimated.Results:For the regimen 0.5 mg/kg/day/7 days were identified 7 studies, which showed an incidence of recurrence between 0% and 20% with follow-up 60-210 days; only 4 studies comparing with the standard regimen 0.25 mg/kg/day/14 days and no difference in recurrences between both regimens (RR= 0.977, 95% CI= 0.670 to 1.423 were found. 3 clinical trials using regimen 0.5 mg/kg/day/14 days with an incidence of recurrences between 1.8% and 18.0% during 330-365 days were identified; only one study comparing with the standard regimen (RR= 0.846, 95% CI= 0.484 to 1.477. High risk of bias and differences in handling of included studies were found.Conclusion:Available evidence is insufficient to determine whether currently PQ regimens used as alternative rather than standard treatment have better efficacy and safety in preventing relapse of P. vivax. Clinical trials are required to guide changes in treatment regimen of malaria vivax.

  2. Structural and Functional Analysis of a C3b-specific Antibody That Selectively Inhibits the Alternative Pathway of Complement*S⃞

    OpenAIRE

    Katschke, Kenneth J.; Stawicki, Scott; Yin, JianPing; Steffek, Micah; Xi, Hongkang; Sturgeon, Lizette; Hass, Philip E.; Loyet, Kelly M.; DeForge, Laura; Wu, Yan; van Lookeren Campagne, Menno; Wiesmann, Christian

    2009-01-01

    Amplification of the complement cascade through the alternative pathway can lead to excessive inflammation. Targeting C3b, a component central to the alternative pathway of complement, provides a powerful approach to inhibit complement-mediated immune responses and tissue injury. In the present study, phage display technology was employed to generate an antibody that selectively recognizes C3b but not the non-activated molecule C3. The crystal structure of C3b in compl...

  3. Lawrence Livermore National Laboratory Decontamination and Waste Treatment Facility: Documentation of impact analysis for design alternatives presented in the Draft Environmental Impact Statement

    International Nuclear Information System (INIS)

    Lawrence Livermore National Laboratory (LLNL) is proposing to construct and operate a new Decontamination and Waste Treatment Facility (DWTF). The proposed DWTF would replace the existing Hazardous Waste Management (HWM) facilities at LLNL. The US Department of Energy (DOE) is preparing a Draft Environmental Impact Statement (DEIS) to assess the environmental consequences of the proposed DWTF and its alternatives. This report presents the assumptions, methodologies, and analyses used to estimate the waste flows, air emissions, ambient air quality impacts, and public health risks that are presented in the DEIS. Two DWTF design alternatives (Level I and Level II) have been designated as reasonable design alternatives considering available technologies, environmental regulations, and current and future LLNL waste generation. Both design alternatives would include new, separate radioactive and nonradioactive liquid waste treatment systems, a solidification unit, a new decontamination facility, storage and treatment facilities for reactive materials, a radioactive waste storage area, receiving and classification areas, and a uranium burn pan. The Level I design alternative would include a controlled-air incinerator system, while the Level II design alternative would include a rotary kiln incinerator system. 43 refs., 4 figs., 24 tabs

  4. Mutational analysis of the Trypanosoma vivax alternative oxidase: The E(X)6Y motif is conserved in both mitochondrial alternative oxidase and plastid terminal oxidase and is indispensable for enzyme activity

    International Nuclear Information System (INIS)

    Based on amino acid sequence similarity and the ability to catalyze the four-electron reduction of oxygen to water using a quinol substrate, mitochondrial alternative oxidase (AOX) and plastid terminal oxidase (PTOX) appear to be two closely related members of the membrane-bound diiron carboxylate group of proteins. In the current studies, we took advantage of the high activity of Trypanosoma vivax AOX (TvAOX) to examine the importance of the conserved Glu and the Tyr residues around the predicted third helix region of AOXs and PTOXs. We first compared the amino acid sequences of TvAOX with AOXs and PTOXs from various taxa and then performed alanine-scanning mutagenesis of TvAOX between amino acids Y199 and Y247. We found that the ubiquinol oxidase activity of TvAOX is completely lost in the E214A mutant, whereas mutants E215A and E216A retained more than 30% of the wild-type activity. Among the Tyr mutants, a complete loss of activity was also observed for the Y221A mutant, whereas the activities were equivalent to wild-type for the Y199A, Y212A, and Y247A mutants. Finally, residues Glu214 and Tyr221 were found to be strictly conserved among AOXs and PTOXs. Based on these findings, it appears that AOXs and PTOXs are a novel subclass of diiron carboxylate proteins that require the conserved motif E(X)6Y for enzyme activity

  5. Beyond the transect: An alternative microchemical imaging method for fine scale analysis of trace elements in fish otoliths during early life

    International Nuclear Information System (INIS)

    has no significant advantage over line scans. - Highlights: • Ba and Sr levels in otoliths from P. coelestis were imaged using LA-ICP-MS. • Longerich's calibration method was applied to quantitative otolith imaging. • An alternative to single line transects for microchemical analysis is presented

  6. Beyond the transect: An alternative microchemical imaging method for fine scale analysis of trace elements in fish otoliths during early life

    Energy Technology Data Exchange (ETDEWEB)

    McGowan, Nicole [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); CCFS ARC Centre of Excellence, Department of Earth and Planetary Sciences, Macquarie University, Sydney (Australia); Fowler, Ashley M.; Parkinson, Kerryn [Fish Ecology Laboratory, School of the Environment, University of Technology, Sydney, New South Wales (Australia); Bishop, David P. [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); Ganio, Katherine [Florey Department of Neuroscience and Mental Health, University of Melbourne, Victoria (Australia); Doble, Philip A. [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); Booth, David J. [Fish Ecology Laboratory, School of the Environment, University of Technology, Sydney, New South Wales (Australia); Hare, Dominic J., E-mail: dominic.hare@uts.edu.au [Elemental Bio-imaging Facility, University of Technology, Sydney, New South Wales (Australia); Florey Department of Neuroscience and Mental Health, University of Melbourne, Victoria (Australia)

    2014-10-01

    scanning has no significant advantage over line scans. - Highlights: • Ba and Sr levels in otoliths from P. coelestis were imaged using LA-ICP-MS. • Longerich's calibration method was applied to quantitative otolith imaging. • An alternative to single line transects for microchemical analysis is presented.

  7. Vehicle Design Evaluation Program (VDEP). A computer program for weight sizing, economic, performance and mission analysis of fuel-conservative aircraft, multibodied aircraft and large cargo aircraft using both JP and alternative fuels

    Science.gov (United States)

    Oman, B. H.

    1977-01-01

    The NASA Langley Research Center vehicle design evaluation program (VDEP-2) was expanded by (1) incorporating into the program a capability to conduct preliminary design studies on subsonic commercial transport type aircraft using both JP and such alternate fuels as hydrogen and methane;(2) incorporating an aircraft detailed mission and performance analysis capability; and (3) developing and incorporating an external loads analysis capability. The resulting computer program (VDEP-3) provides a preliminary design tool that enables the user to perform integrated sizing, structural analysis, and cost studies on subsonic commercial transport aircraft. Both versions of the VDEP-3 Program which are designated preliminary Analysis VDEP-3 and detailed Analysis VDEP utilize the same vehicle sizing subprogram which includes a detailed mission analysis capability, as well as a geometry and weight analysis for multibodied configurations.

  8. Potential of renewable and alternative energy sources

    OpenAIRE

    Konovalov, Vyacheslav Vasilievich; Pozharnitskaya, Olga Vyacheslavovna; Rostovshchikova, А.; Matveenko, Irina Alekseevna

    2015-01-01

    The article deals with application potential of clean alternative renewable energy sources. By means of system analysis the forecast for consumption of electrical energy in Tomsk Oblast as well as main energy sources of existing energy system have been studied up to 2018. Engineering potential of renewable and alternative energy sources is evaluated. Besides, ranking in the order of their efficiency descending is performed. It is concluded that Tomsk Oblast has high potential of alternative a...

  9. Methods for Characterization of Alternative RNA Splicing.

    Science.gov (United States)

    Harvey, Samuel E; Cheng, Chonghui

    2016-01-01

    Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing "minigene" in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest.

  10. Nuclear energy: a sensible alternative

    International Nuclear Information System (INIS)

    This book presents in-depth articles on the main issues affecting the use and usefulness of nuclear energy for peaceful domestic purposes. Topics considered include energy futures (a world study), energy demand-energy supplies, an energy-deficient society, energy shortages, the economics of light water reactors (LWRs), fast breeder reactor economics, international cooperation in the nuclear field, nuclear recycling (costs, savings, safeguards), alternative fuels, alternative fuel cycles, alternative reactors, the nuclear weapons proliferation issue, paths to a world with more reliable nuclear safeguards, the homemade bomb issue, LWR risk assessment, accident analysis and risk assessment, the waste disposal risk, radon problems, health effects of low-level radiation, routine releases of radioactivity, plutonium toxicity, and the Price-Anderson Act

  11. Support vector machine regression (LS-SVM)--an alternative to artificial neural networks (ANNs) for the analysis of quantum chemistry data?

    Science.gov (United States)

    Balabin, Roman M; Lomakina, Ekaterina I

    2011-06-28

    A multilayer feed-forward artificial neural network (MLP-ANN) with a single, hidden layer that contains a finite number of neurons can be regarded as a universal non-linear approximator. Today, the ANN method and linear regression (MLR) model are widely used for quantum chemistry (QC) data analysis (e.g., thermochemistry) to improve their accuracy (e.g., Gaussian G2-G4, B3LYP/B3-LYP, X1, or W1 theoretical methods). In this study, an alternative approach based on support vector machines (SVMs) is used, the least squares support vector machine (LS-SVM) regression. It has been applied to ab initio (first principle) and density functional theory (DFT) quantum chemistry data. So, QC + SVM methodology is an alternative to QC + ANN one. The task of the study was to estimate the Møller-Plesset (MPn) or DFT (B3LYP, BLYP, BMK) energies calculated with large basis sets (e.g., 6-311G(3df,3pd)) using smaller ones (6-311G, 6-311G*, 6-311G**) plus molecular descriptors. A molecular set (BRM-208) containing a total of 208 organic molecules was constructed and used for the LS-SVM training, cross-validation, and testing. MP2, MP3, MP4(DQ), MP4(SDQ), and MP4/MP4(SDTQ) ab initio methods were tested. Hartree-Fock (HF/SCF) results were also reported for comparison. Furthermore, constitutional (CD: total number of atoms and mole fractions of different atoms) and quantum-chemical (QD: HOMO-LUMO gap, dipole moment, average polarizability, and quadrupole moment) molecular descriptors were used for the building of the LS-SVM calibration model. Prediction accuracies (MADs) of 1.62 ± 0.51 and 0.85 ± 0.24 kcal mol(-1) (1 kcal mol(-1) = 4.184 kJ mol(-1)) were reached for SVM-based approximations of ab initio and DFT energies, respectively. The LS-SVM model was more accurate than the MLR model. A comparison with the artificial neural network approach shows that the accuracy of the LS-SVM method is similar to the accuracy of ANN. The extrapolation and interpolation results show that LS-SVM is

  12. Support vector machine regression (LS-SVM)--an alternative to artificial neural networks (ANNs) for the analysis of quantum chemistry data?

    Science.gov (United States)

    Balabin, Roman M; Lomakina, Ekaterina I

    2011-06-28

    A multilayer feed-forward artificial neural network (MLP-ANN) with a single, hidden layer that contains a finite number of neurons can be regarded as a universal non-linear approximator. Today, the ANN method and linear regression (MLR) model are widely used for quantum chemistry (QC) data analysis (e.g., thermochemistry) to improve their accuracy (e.g., Gaussian G2-G4, B3LYP/B3-LYP, X1, or W1 theoretical methods). In this study, an alternative approach based on support vector machines (SVMs) is used, the least squares support vector machine (LS-SVM) regression. It has been applied to ab initio (first principle) and density functional theory (DFT) quantum chemistry data. So, QC + SVM methodology is an alternative to QC + ANN one. The task of the study was to estimate the Møller-Plesset (MPn) or DFT (B3LYP, BLYP, BMK) energies calculated with large basis sets (e.g., 6-311G(3df,3pd)) using smaller ones (6-311G, 6-311G*, 6-311G**) plus molecular descriptors. A molecular set (BRM-208) containing a total of 208 organic molecules was constructed and used for the LS-SVM training, cross-validation, and testing. MP2, MP3, MP4(DQ), MP4(SDQ), and MP4/MP4(SDTQ) ab initio methods were tested. Hartree-Fock (HF/SCF) results were also reported for comparison. Furthermore, constitutional (CD: total number of atoms and mole fractions of different atoms) and quantum-chemical (QD: HOMO-LUMO gap, dipole moment, average polarizability, and quadrupole moment) molecular descriptors were used for the building of the LS-SVM calibration model. Prediction accuracies (MADs) of 1.62 ± 0.51 and 0.85 ± 0.24 kcal mol(-1) (1 kcal mol(-1) = 4.184 kJ mol(-1)) were reached for SVM-based approximations of ab initio and DFT energies, respectively. The LS-SVM model was more accurate than the MLR model. A comparison with the artificial neural network approach shows that the accuracy of the LS-SVM method is similar to the accuracy of ANN. The extrapolation and interpolation results show that LS-SVM is

  13. Review of bioinformatics data analysis in alternative splicing%可变剪接的生物信息数据分析综述

    Institute of Scientific and Technical Information of China (English)

    章天骄

    2012-01-01

    前体mRNA的可变剪接是扩大真核生物蛋白质组多样性的重要基因调控机制.可变剪接的错误调节可以引起多种人类疾病.由于高通量技术的发展,生物信息学成为可变剪接研究的主要手段.本文总结了可变剪接在生物信息学领域的研究方法,同时也分析并预测了可变剪接的发展方向.%Alternative pre - mRNA splicing is an important gene regulation mechanism for expanding proteomic diversity in higher eukaryotes. The misregulation of alternative splicing underlies many human diseases. With the development of high - throughput technology, bioinformatics becomes to the main method in study of alternative splicing. This article summarizes the bioinformatics methods in alternative splicing research, as well as analyzes and predicts the direction of alternative splicing.

  14. Alternative energies. Updates on progress

    International Nuclear Information System (INIS)

    Presents fundamental and applied research of alternative energies. Address key pillars in the alternative energy field, such as: biomass energy, hydrogen energy, solar energy, wind energy, hydroelectric power, geothermal energy and their environmental implications, with the most updated progress. Includes the life cycle assessment and thermoeconomic analysis as tools for evaluating and optimising environmental and cost subjects. This book presents nine chapters based on fundamental and applied research of alternative energies. At the present time, the challenge is that technology has to come up with solutions that can provide environmentally friendly energy supply options that are able to cover the current world energy demand. Experts around the world are working on these issues for providing new solutions that will break the existing technological barriers. This book aims to address key pillars in the alternative energy field, such as: biomass energy, hydrogen energy, solar energy, wind energy, hydroelectric power, geothermal energy and their environmental implications, with the most updated progress for each pillar. It also includes the life cycle assessment (LCA) and thermoeconomic analysis (TA) as tools for evaluating and optimising environmental and cost subjects. Chapters are organized into fundamental research, applied research and future trends; and written for engineers, academic researches and scientists.

  15. Alternative energies. Updates on progress

    Energy Technology Data Exchange (ETDEWEB)

    Ferreira, German (ed.) [CIRCE - Centre of Research for Energy Resources and Consumption, Zaragoza (Spain)

    2013-07-01

    Presents fundamental and applied research of alternative energies. Address key pillars in the alternative energy field, such as: biomass energy, hydrogen energy, solar energy, wind energy, hydroelectric power, geothermal energy and their environmental implications, with the most updated progress. Includes the life cycle assessment and thermoeconomic analysis as tools for evaluating and optimising environmental and cost subjects. This book presents nine chapters based on fundamental and applied research of alternative energies. At the present time, the challenge is that technology has to come up with solutions that can provide environmentally friendly energy supply options that are able to cover the current world energy demand. Experts around the world are working on these issues for providing new solutions that will break the existing technological barriers. This book aims to address key pillars in the alternative energy field, such as: biomass energy, hydrogen energy, solar energy, wind energy, hydroelectric power, geothermal energy and their environmental implications, with the most updated progress for each pillar. It also includes the life cycle assessment (LCA) and thermoeconomic analysis (TA) as tools for evaluating and optimising environmental and cost subjects. Chapters are organized into fundamental research, applied research and future trends; and written for engineers, academic researches and scientists.

  16. On an Alternative Cosmology

    CERN Document Server

    Vankov, A

    1998-01-01

    The suggested alternative cosmology is based on the idea of barion symmetric universe, in which our home universe is a representative of multitude of typical matter and antimatter universes. This alternative concept gives a physically reasonable explanation of all major problems of the Standard Cosmological Model. Classification Code MSC: Cosmology 524.8 Key words: standard cosmological model, alternative cosmology, barionic symmetry, typical universe, quasars, cosmic rays.

  17. Polyphase alternating codes

    CERN Document Server

    Markkanen, Markku

    2007-01-01

    This work introduces a method for constructing polyphase alternating codes in which the length of a code transmission cycle can be $p^m$ or $p-1$, where $p$ is a prime number and $m$ is a positive integer. The relevant properties leading to the construction alternating codes and the algorithm for generating alternating codes is described. Examples of all practical and some not that practical polyphase code lengths are given.

  18. Alternative Solar Indices

    Energy Technology Data Exchange (ETDEWEB)

    Lantz, L.J.

    1980-07-01

    Possible alternative Solar Indices which could either be a perturbation from the currently defined Solar Index or possible indices based on current technologies for other media markets are discussed. An overview is given of the current project, including the logic that was utilized in defining its current structure and then alternative indices and definitions are presented and finally, recommendations are made for adopting alternative indices.

  19. Complex Alternative Splicing

    OpenAIRE

    Park, Jung Woo; Graveley, Brenton R.

    2007-01-01

    Alternative splicing is a powerful means of controlling gene expression and increasing protein diversity. Most genes express a limited number of mRNA isoforms, but there are several examples of genes that use alternative splicing to generate hundreds, thousands, and even tens of thousands of isoforms. Collectively such genes are considered to undergo complex alternative splicing. The best example is the Drosophila Down syndrome cell adhesion molecule (Dscam) gene, which can generate 38,016 is...

  20. Analysis of nitrogen injection as alternative fluid to steam in heavy oil reservoir; Analise da injecao de nitrogenio como fluido alternativo ao vapor em reservatorio de oleo pesado

    Energy Technology Data Exchange (ETDEWEB)

    Rodrigues, Marcos Allyson Felipe; Galvao, Edney Rafael Viana Pinheiro; Barillas, Jennys Lourdes; Mata, Wilson da; Dutra Junior, Tarcilio Viana [Universidade Federal do Rio Grande do Norte (UFRN), RN (Brazil)

    2012-07-01

    Many of hydrocarbon reserves existing in the world are formed by heavy oils (deg API between 10 and 20). Moreover, several heavy oil fields are mature and, thus, offer great challenges for oil industry. Among the thermal methods used to recover these resources, steam flooding has been the main economically viable alternative. Latent heat carried by steam heats the reservoir, reducing oil viscosity and facilitating the production. This method has many variations and has been studied both theoretically and experimentally (in pilot projects and in full field applications). In order to increase oil recovery and reduce steam injection costs, the injection of alternative fluid has been used on three main ways: alternately, co-injected with steam and after steam injection interruption. The main objective of these injection systems is to reduce the amount of heat supplied to the reservoir, using cheaper fluids and maintaining the same oil production levels. In this paper, the use of N{sub 2} as an alternative fluid to the steam was investigated. The analyzed parameters were oil recoveries and net cumulative oil productions. The reservoir simulation model corresponds to an oil reservoir of 100 m x 100 m x 28 m size, on a Cartesian coordinates system (x, y and z directions). It is a semi synthetic model with some reservoir data similar to those found in Potiguar Basin, Brazil. All studied cases were done using the simulator STARS from CMG (Computer Modelling Group, version 2009.10). It was found that N{sub 2} injection after steam injection interruption achieved the highest net cumulative oil compared to others injection system. Moreover, it was observed that N2 as alternative fluid to steam did not present increase on oil recovery. (author)

  1. FMDP reactor alternative summary report: Volume 4, Evolutionary LWR alternative

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-09-01

    Significant quantities of weapons-usable fissile materials [primarily plutonium and highly enriched uranium (HEU)] have become surplus to national defense needs both in the United States and Russia. These stocks of fissile materials pose significant dangers to national and international security. The dangers exist not only in the potential proliferation of nuclear weapons but also in the potential for environmental, safety, and health (ES&H) consequences if surplus fissile materials are not properly managed. The purpose of this report is to provide schedule, cost, and technical information that will be used to support the Record of Process (ROD). Following the screening process, DOE/MD via its national laboratories initiated a more detailed analysis activity to further evaluate each of the ten plutonium disposition alternatives that survived the screening process. Three ``Alternative Teams,`` chartered by DOE and comprised of technical experts from across the DOE national laboratory complex, conducted these analyses. One team was chartered for each of the major disposition classes (borehole, immobilization, and reactors). During the last year and a half, the Fissile Materials Disposition Program (FMDP) Reactor Alternative Team (RxAT) has conducted extensive analyses of the cost, schedule, technical maturity, S&S, and other characteristics of reactor-based plutonium disposition. The results of the RxAT`s analyses of the existing LWR, CANDU, and partially complete LWR alternatives are documented in Volumes 1-3 of this report. This document (Volume 4) summarizes the results of these analyses for the ELWR-based plutonium disposition option.

  2. FMDP reactor alternative summary report: Volume 4, Evolutionary LWR alternative

    International Nuclear Information System (INIS)

    Significant quantities of weapons-usable fissile materials [primarily plutonium and highly enriched uranium (HEU)] have become surplus to national defense needs both in the United States and Russia. These stocks of fissile materials pose significant dangers to national and international security. The dangers exist not only in the potential proliferation of nuclear weapons but also in the potential for environmental, safety, and health (ES ampersand H) consequences if surplus fissile materials are not properly managed. The purpose of this report is to provide schedule, cost, and technical information that will be used to support the Record of Process (ROD). Following the screening process, DOE/MD via its national laboratories initiated a more detailed analysis activity to further evaluate each of the ten plutonium disposition alternatives that survived the screening process. Three ''Alternative Teams,'' chartered by DOE and comprised of technical experts from across the DOE national laboratory complex, conducted these analyses. One team was chartered for each of the major disposition classes (borehole, immobilization, and reactors). During the last year and a half, the Fissile Materials Disposition Program (FMDP) Reactor Alternative Team (RxAT) has conducted extensive analyses of the cost, schedule, technical maturity, S ampersand S, and other characteristics of reactor-based plutonium disposition. The results of the RxAT's analyses of the existing LWR, CANDU, and partially complete LWR alternatives are documented in Volumes 1-3 of this report. This document (Volume 4) summarizes the results of these analyses for the ELWR-based plutonium disposition option

  3. Alternative Devices for Taking Insulin

    Science.gov (United States)

    ... KB). Alternate Language URL Alternative Devices for Taking Insulin Page Content On this page: What alternative devices ... the skin. [ Top ] What alternative devices for taking insulin are available? Insulin pens provide a convenient, easy- ...

  4. Alternative health insurance schemes

    DEFF Research Database (Denmark)

    Keiding, Hans; Hansen, Bodil O.

    2002-01-01

    In this paper, we present a simple model of health insurance with asymmetric information, where we compare two alternative ways of organizing the insurance market. Either as a competitive insurance market, where some risks remain uninsured, or as a compulsory scheme, where however, the level...... competitive insurance; this situation turns out to be at least as good as either of the alternatives...

  5. Economic analysis of alternative bison and elk management practices on the National Elk Refuge and Grand Teton National Park: A comparison of visitor and household responses

    Science.gov (United States)

    Loomis, John B.; Caughlan, Lynne

    2004-01-01

    The U.S. Fish and Wildlife Service (FWS) and the National Park Service (NPS) are preparing a management plan for bison and elk inhabiting the National Elk Refuge (NER) and Grand Teton National Park (GTNP). These animals are part of the bison and elk herds in Jackson Hole, one of the largest concentrations of freeranging bison and elk in the world. A range of alternatives for managing the bison and elk herds in the project area will be developed in an Environmental Impact Statement. The EIS will likely include such issues as habitat management, disease management, winter-feeding and hunting programs related to the NER and GTNP. The purposes of this study are to determine how the current management and EIS alternatives for bison and elk inhabiting the NER and GTNP would change: Visitor use

  6. Comparative analysis of serine/arginine-rich proteins across 27 eukaryotes: insights into sub-family classification and extent of alternative splicing.

    Directory of Open Access Journals (Sweden)

    Dale N Richardson

    Full Text Available Alternative splicing (AS of pre-mRNA is a fundamental molecular process that generates diversity in the transcriptome and proteome of eukaryotic organisms. SR proteins, a family of splicing regulators with one or two RNA recognition motifs (RRMs at the N-terminus and an arg/ser-rich domain at the C-terminus, function in both constitutive and alternative splicing. We identified SR proteins in 27 eukaryotic species, which include plants, animals, fungi and "basal" eukaryotes that lie outside of these lineages. Using RNA recognition motifs (RRMs as a phylogenetic marker, we classified 272 SR genes into robust sub-families. The SR gene family can be split into five major groupings, which can be further separated into 11 distinct sub-families. Most flowering plants have double or nearly double the number of SR genes found in vertebrates. The majority of plant SR genes are under purifying selection. Moreover, in all paralogous SR genes in Arabidopsis, rice, soybean and maize, one of the two paralogs is preferentially expressed throughout plant development. We also assessed the extent of AS in SR genes based on a splice graph approach (http://combi.cs.colostate.edu/as/gmap_SRgenes. AS of SR genes is a widespread phenomenon throughout multiple lineages, with alternative 3' or 5' splicing events being the most prominent type of event. However, plant-enriched sub-families have 57%-88% of their SR genes experiencing some type of AS compared to the 40%-54% seen in other sub-families. The SR gene family is pervasive throughout multiple eukaryotic lineages, conserved in sequence and domain organization, but differs in gene number across lineages with an abundance of SR genes in flowering plants. The higher number of alternatively spliced SR genes in plants emphasizes the importance of AS in generating splice variants in these organisms.

  7. Analysis of the Isoprenoid Biosynthesis Pathways in Listeria monocytogenes Reveals a Role for the Alternative 2-C-Methyl-d-Erythritol 4-Phosphate Pathway in Murine Infection▿

    OpenAIRE

    Begley, Máire; Bron, Peter A; Heuston, Sinead; Casey, Pat G.; Englert, Nadine; Wiesner, Jochen; Jomaa, Hassan; Gahan, Cormac G. M.; Hill, Colin

    2008-01-01

    Most bacteria synthesize isoprenoids through one of two essential pathways which provide the basic building block, isopentyl diphosphate (IPP): either the classical mevalonate pathway or the alternative non-mevalonate 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway. However, postgenomic analyses of the Listeria monocytogenes genome revealed that this pathogen possesses the genetic capacity to produce the complete set of enzymes involved in both pathways. The nonpathogenic species Listeria i...

  8. An analysis of Alternative Dispute Resolution (ADR) as it applies to contract dispute settlement and its use by the Defense Industry

    OpenAIRE

    Begin, Marc A.

    1999-01-01

    The objective of this research is to assess the extent to which defense contractors employ the use of Alternative Dispute Resolution (ADR) in their contract dispute settlement processes. A questionnaire was utilized to gather information from thirty defense contractors. The thesis provides a legislative background for ADR, and briefly discusses various techniques of the ADR process. Additionally, data collected from industry are presented and discussed. The study identified mediation and nego...

  9. Alternative loop rings

    CERN Document Server

    Goodaire, EG; Polcino Milies, C

    1996-01-01

    For the past ten years, alternative loop rings have intrigued mathematicians from a wide cross-section of modern algebra. As a consequence, the theory of alternative loop rings has grown tremendously. One of the main developments is the complete characterization of loops which have an alternative but not associative, loop ring. Furthermore, there is a very close relationship between the algebraic structures of loop rings and of group rings over 2-groups. Another major topic of research is the study of the unit loop of the integral loop ring. Here the interaction between loop rings and group ri

  10. HL-LHC alternatives

    CERN Document Server

    Tomás, R; White, S

    2014-01-01

    The HL-LHC parameters assume unexplored regimes for hadron colliders in various aspects of accelerator beam dynamics and technology. This paper reviews three alternatives that could potentially improve the LHC performance: (i) the alternative filling scheme 8b+4e, (ii) the use of a 200 MHz RF system in the LHC and (iii) the use of proton cooling methods to reduce the beam emittance (at top energy and at injection). The alternatives are assessed in terms of feasibility, pros and cons, risks versus benefits and the impact on beam availability.

  11. Seal design alternatives study

    International Nuclear Information System (INIS)

    This report presents the results from a study of various sealing alternatives for the WIPP sealing system. Overall, the sealing system has the purpose of reducing to the extent possible the potential for fluids (either gas or liquid) from entering or leaving the repository. The sealing system is divided into three subsystems: drift and panel seals within the repository horizon, shaft seals in each of the four shafts, and borehole seals. Alternatives to the baseline configuration for the WIPP seal system design included evaluating different geometries and schedules for seal component installations and the use of different materials for seal components. Order-of-magnitude costs for the various alternatives were prepared as part of the study. Firm recommendations are not presented, but the advantages and disadvantages of the alternatives are discussed. Technical information deficiencies are identified and studies are outlined which can provide required information

  12. Evaluation of Expenditure Alternates

    Science.gov (United States)

    Poehlein, Gary W.; And Others

    1973-01-01

    Illustrates a system of calculating dollar expenditures over periods of time in terms of present value. The system enables planners, school boards, and administrators to compare expenditure alternatives as a decisionmaking factor. (Author)

  13. Alternatives to Nursing Homes

    Science.gov (United States)

    ... this website may not be available. Alternatives to nursing homes Before you make any decisions about long ... live and what help you may need. A nursing home may not be your only choice. Discharge ...

  14. Alternative disinfectant water treatments

    Science.gov (United States)

    Alternative disinfestant water treatments are disinfestants not as commonly used by the horticultural industry. Chlorine products that produce hypochlorous acid are the main disinfestants used for treating irrigation water. Chlorine dioxide will be the primary disinfestant discussed as an alternativ...

  15. Vaginal dryness alternative treatments

    Science.gov (United States)

    ... a day. Soybeans contain plant-based substances called isoflavones. These substances have an effect on the body ... soy for vasomotor symptoms: the Herbal Alternatives for Menopause (HALT) Study. Menopause . 2008;15(1):51-58. ...

  16. An alternative currency

    OpenAIRE

    Frendo, Mario; Duca, Edward

    2013-01-01

    Malta- a tiny island, a minute social reality, a precursory canovaccio of European unification- has a unique asset it ought to be prouder of: culture. http://www.um.edu.mt/think/an-alternative-currency/

  17. Alternative fuel information sources

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-01

    This short document contains a list of more than 200 US sources of information (Name, address, phone number, and sometimes contact) related to the use of alternative fuels in automobiles and trucks. Electric-powered cars are also included.

  18. Seal design alternatives study

    Energy Technology Data Exchange (ETDEWEB)

    Van Sambeek, L.L. [RE/SPEC Inc., Rapid City, SD (US); Luo, D.D.; Lin, M.S.; Ostrowski, W.; Oyenuga, D. [Parsons Brinckerhoff Quade & Douglas, Inc., San Francisco, CA (US)

    1993-06-01

    This report presents the results from a study of various sealing alternatives for the WIPP sealing system. Overall, the sealing system has the purpose of reducing to the extent possible the potential for fluids (either gas or liquid) from entering or leaving the repository. The sealing system is divided into three subsystems: drift and panel seals within the repository horizon, shaft seals in each of the four shafts, and borehole seals. Alternatives to the baseline configuration for the WIPP seal system design included evaluating different geometries and schedules for seal component installations and the use of different materials for seal components. Order-of-magnitude costs for the various alternatives were prepared as part of the study. Firm recommendations are not presented, but the advantages and disadvantages of the alternatives are discussed. Technical information deficiencies are identified and studies are outlined which can provide required information.

  19. Alternative fuel cycles

    International Nuclear Information System (INIS)

    Uranium resource utilization and economic considerations provide incentives to study alternative fuel cycles as future options to the PHWR natural uranium cycle. Preliminary studies to define the most favourable alternatives and their possible introduction dates are discussed. The important and uncertain components which influence option selection are reviewed, including nuclear capacity growth, uranium availability and demand, economic potential, and required technological developments. Finally, a summary of Ontario Hydro's program to further assess cycle selection and define development needs is given. (auth)

  20. Genetic analysis of complement C1s deficiency associated with systemic lupus erythematosus highlights alternative splicing of normal C1s gene

    DEFF Research Database (Denmark)

    Armano, MT; Ferriani, VP; Florido, MP;

    2008-01-01

    ' fibroblasts when analyzed by confocal microscopy. We show that all four siblings are homozygous for a mutation at position 938 in exon 6 of the C1s cDNA that creates a premature stop codon. Our investigations led us to reveal the presence of previously uncharacterized splice variants of C1s mRNA transcripts...... in normal human cells. These variants are derived from the skipping of exon 3 and from the use of an alternative 3' splice site within intron 1 which increases the size of exon 2 by 87 nucleotides....

  1. An alternative easy method for antibody purification and analysis of protein-protein interaction using GST fusion proteins immobilized onto glutathione-agarose.

    Science.gov (United States)

    Zalazar, L; Alonso, C A I; De Castro, R E; Cesari, A

    2014-01-01

    Immobilization of small proteins designed to perform protein-protein assays can be a difficult task. Often, the modification of reactive residues necessary for the interaction between the immobilized protein and the matrix compromises the interaction between the protein and its target. In these cases, glutathione-S-transferase (GST) is a valuable tag providing a long arm that makes the bait protein accessible to the mobile flow phase of the chromatography. In the present report, we used a GST fusion version of the 8-kDa protein serine protease inhibitor Kazal-type 3 (SPINK3) as the bait to purify anti-SPINK3 antibodies from a rabbit crude serum. The protocol for immobilization of GST-SPINK3 to glutathione-agarose beads was modified from previously reported protocols by using an alternative bifunctional cross-linker (dithiobis(succinimidyl propionate)) in a very simple procedure and by using simple buffers under physiological conditions. We concluded that the immobilized protein remained bound to the column after elution with low pH, allowing the reuse of the column for alternative uses, such as screening for other protein-protein interactions using SPINK3 as the bait.

  2. WTAQ version 2-A computer program for analysis of aquifer tests in confined and water-table aquifers with alternative representations of drainage from the unsaturated zone

    Science.gov (United States)

    Barlow, Paul M.; Moench, Allen F.

    2011-01-01

    The computer program WTAQ simulates axial-symmetric flow to a well pumping from a confined or unconfined (water-table) aquifer. WTAQ calculates dimensionless or dimensional drawdowns that can be used with measured drawdown data from aquifer tests to estimate aquifer hydraulic properties. Version 2 of the program, which is described in this report, provides an alternative analytical representation of drainage to water-table aquifers from the unsaturated zone than that which was available in the initial versions of the code. The revised drainage model explicitly accounts for hydraulic characteristics of the unsaturated zone, specifically, the moisture retention and relative hydraulic conductivity of the soil. The revised program also retains the original conceptualizations of drainage from the unsaturated zone that were available with version 1 of the program to provide alternative approaches to simulate the drainage process. Version 2 of the program includes all other simulation capabilities of the first versions, including partial penetration of the pumped well and of observation wells and piezometers, well-bore storage and skin effects at the pumped well, and delayed drawdown response of observation wells and piezometers.

  3. The usefulness of fungicide mixtures and alternation for delaying the selection for resistance in populations of Mycosphaerella graminicola on winter wheat: a modeling analysis.

    Science.gov (United States)

    Hobbelen, P H F; Paveley, N D; Oliver, R P; van den Bosch, F

    2013-07-01

    A fungicide resistance model (reported and tested previously) was amended to describe the development of resistance in Mycosphaerella graminicola populations in winter wheat (Triticum aestivum) crops in two sets of fields, connected by spore dispersal. The model was used to evaluate the usefulness of concurrent, alternating, or mixture use of two high-resistance-risk fungicides as resistance management strategies. We determined the effect on the usefulness of each strategy of (i) fitness costs of resistance, (ii) partial resistance to fungicides, (iii) differences in the dose-response curves and decay rates between fungicides, and (iv) different frequencies of the double-resistant strain at the start of a treatment strategy. Parameter values for the quinine outside inhibitor pyraclostrobin were used to represent two fungicides with differing modes of action. The effectiveness of each strategy was quantified as the maximum number of growing seasons that disease was effectively controlled in both sets of fields. For all scenarios, the maximum effective lives achieved by the use of the strategies were in the order mixtures ≥ alternation ≥ concurrent use. Mixtures were of particular benefit where the pathogen strain resistant to both modes of action incurred a fitness penalty or was present at a low initial frequency.

  4. FMDP Reactor Alternative Summary Report: Volume 2 - CANDU heavy water reactor alternative

    International Nuclear Information System (INIS)

    The Department of Energy Office of Fissile Materials Disposition (DOE/MD) initiated a detailed analysis activity to evaluate each of ten plutonium disposition alternatives that survived an initial screening process. This document, Volume 2 of a four volume report, summarizes the results of these analyses for the CANDU reactor based plutonium disposition alternative

  5. FMDP Reactor Alternative Summary Report: Volume 3 - partially complete LWR alternative

    International Nuclear Information System (INIS)

    The Department of Energy Office of Fissile Materials Disposition (DOE/MD) initiated a detailed analysis activity to evaluate each of ten plutonium disposition alternatives that survived an initial screening process. This document, Volume 3 of a four volume report summarizes the results of these analyses for the partially complete LWR (PCLWR) reactor based plutonium disposition alternative

  6. FMDP Reactor Alternative Summary Report: Volume 2 - CANDU heavy water reactor alternative

    Energy Technology Data Exchange (ETDEWEB)

    Greene, S.R.; Spellman, D.J.; Bevard, B.B. [and others

    1996-09-01

    The Department of Energy Office of Fissile Materials Disposition (DOE/MD) initiated a detailed analysis activity to evaluate each of ten plutonium disposition alternatives that survived an initial screening process. This document, Volume 2 of a four volume report, summarizes the results of these analyses for the CANDU reactor based plutonium disposition alternative.

  7. FMDP reactor alternative summary report. Volume 1 - existing LWR alternative

    International Nuclear Information System (INIS)

    Significant quantities of weapons-usable fissile materials [primarily plutonium and highly enriched uranium (HEU)] are becoming surplus to national defense needs in both the United States and Russia. These stocks of fissile materials pose significant dangers to national and international security. The dangers exist not only in the potential proliferation of nuclear weapons but also in the potential for environmental, safety, and health (ES ampersand H) consequences if surplus fissile materials are not properly managed. This document summarizes the results of analysis concerned with existing light water reactor plutonium disposition alternatives

  8. FMDP reactor alternative summary report. Volume 1 - existing LWR alternative

    Energy Technology Data Exchange (ETDEWEB)

    Greene, S.R.; Bevard, B.B. [and others

    1996-10-07

    Significant quantities of weapons-usable fissile materials [primarily plutonium and highly enriched uranium (HEU)] are becoming surplus to national defense needs in both the United States and Russia. These stocks of fissile materials pose significant dangers to national and international security. The dangers exist not only in the potential proliferation of nuclear weapons but also in the potential for environmental, safety, and health (ES&H) consequences if surplus fissile materials are not properly managed. This document summarizes the results of analysis concerned with existing light water reactor plutonium disposition alternatives.

  9. Analysis of the Madaras Rotor Power Plant: an alternate method for extracting large amounts of power from the wind. Volume 2. Technical report

    Energy Technology Data Exchange (ETDEWEB)

    Whitford, D.H.; Minardi, J.E.; West, B.S.; Dominic, R.J.

    1978-06-01

    The purpose of the program was to analyze and up-date the design of the Madaras Rotor Power Plant concept that had been developed in the 1930's to determine the technical and economic feasibility of this system to be competitive with conventional horizontal axis wind turbines. A four-task program consisting of a series of wind tunnel tests, an electro-mechanical analysis, a performance analysis, and a cost analysis was conducted.

  10. Innovative and Alternative Technology Assessment Manual

    Energy Technology Data Exchange (ETDEWEB)

    None

    1980-02-01

    This four chapter, six appendix manual presents the procedures and methodology as well as the baseline costs and energy information necessary for the analysis and evaluation of innovative and alternative technology applications submitted for federal grant assistance under the innovative and alternative technology provisions of the Clean Water Act of 1977. The manual clarifies and interprets the intent of Congress and the Environmental Protection Agency in carrying out the mandates of the innovative and alternative provisions of the Clean Water Act of 1977. [DJE 2005

  11. Methods for Characterization of Alternative RNA Splicing

    Science.gov (United States)

    Harvey, Samuel E.; Cheng, Chonghui

    2016-01-01

    Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing “minigene” in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest. PMID:26721495

  12. Potential of renewable and alternative energy sources

    Science.gov (United States)

    Konovalov, V.; Pogharnitskaya, O.; Rostovshchikova, A.; Matveenko, I.

    2015-11-01

    The article deals with application potential of clean alternative renewable energy sources. By means of system analysis the forecast for consumption of electrical energy in Tomsk Oblast as well as main energy sources of existing energy system have been studied up to 2018. Engineering potential of renewable and alternative energy sources is evaluated. Besides, ranking in the order of their efficiency descending is performed. It is concluded that Tomsk Oblast has high potential of alternative and renewable energy sources, among which the most promising development perspective is implementation of gasification stations to save fuel consumed by diesel power stations as well as building wind-power plants.

  13. A socio-anthropological analysis of deficits of transition process in Serbia 2005-2006 and possibilities for elaborating alternative projects

    Directory of Open Access Journals (Sweden)

    Golubović Zagorka

    2006-01-01

    Full Text Available The paper is an outline of the author’s research project to be undertaken in 2006. It is focused on the process of transition from authoritarian to democratic order in Serbia after 2000. Starting from the findings of previous studies of transition, in Serbia and in other post socialist countries, the research will adopt a socio-anthropological approach and deal with the following topics: the model of transition being applied in Serbia preconditions for democratic transformation; a balance sheet of positive achievements accomplished and, on the other hand, "lost chances"; socio economic, political and cultural obstacles to articulating a coherent strategy of democratic development; psychological and anthropological aspects of current processes; alternative paths of democratic development feasible within given conditions. A central question the research will aim at resolving is why the current situation in Serbia is often described as suffering from a "democratic deficit".

  14. CHARACTERISTIC ALTERNATING-DIRECTION FINITE ELEMENT METHODS FOR NONRECTANGULAR REGIONS FOR COUPLED SYSTEM OF DYNAMICS OF FLUIDS IN POROUS MEDIA AND ITS ANALYSIS

    Institute of Scientific and Technical Information of China (English)

    YUAN Yirang

    2005-01-01

    For coupled system of multilayer dynamics of fluids in porous media,the characteristic alternating-direction finite element methods for nonrectangular regions applicable to parallel arithmetic are put forward and two-dimensional and three-dimensional schemes are used to form a complete set. Some techniques,such as calculus of variations,isoparametric transformation,patch approximation,operator-splitting,characteristic method,negative norm estimate,energy method,the theory of prior estimates and techniques are used.For the nonrectangular regions case,optimal order estimates in L2norm are derived for the error in the approximation solution.Thus the well-known theoretical problem has been thoroughly and completely solved.These methods have been successfully used in multilayer oil resources migration-accumulation numerical simulation.

  15. Catalysis for alternative energy generation

    CERN Document Server

    2012-01-01

    Summarizes recent problems in using catalysts in alternative energy generation and proposes novel solutions  Reconsiders the role of catalysis in alternative energy generation  Contributors include catalysis and alternative energy experts from across the globe

  16. Alternative Educational Futures for a Knowledge Society

    Science.gov (United States)

    Young, Michael

    2010-01-01

    This article offers a critical analysis of recent trends in educational policy with particular reference to their assumptions about the knowledge society. It examines the implications of the analysis for the issue of elitism and the promotion of greater educational equality. The article concludes by offering an alternative approach to educational…

  17. Domestic energy alternatives

    International Nuclear Information System (INIS)

    These alternatives include biomass, clean coal, geothermal, hydropower, natural gas, nuclear, solar and photovoltaic, and wind. With the current, volatile situation in the Middle East, this nation's political leaders appear to be left scratching their heads in their attempts to come up with new, sound, energy policies to reduce our dependence on foreign oil. Therefore, the FORTNIGHTLY's editorial staff thought that this might be an opportune time to briefly examine some home-grown and environmentally responsible fuel alternatives to black gold. While some of these electricity-producing technologies are still on the horizon, others are available now

  18. Alternative Practice Dental Hygiene in California: Past, Present, and Future

    OpenAIRE

    MERTZ, ELIZABETH; Glassman, Paul

    2011-01-01

    This study examines the development of the registered dental hygienist in alternative practice in California through an analysis of archival documents, stakeholder interviews, and two surveys of the registered dental hygienist in alternative practice. Designing, testing and implementing a new practice model for dental hygienists took 23 years. Today, registered dental hygienists in alternative practice have developed viable alternative methods for delivering preventive oral health care servic...

  19. The future role of alternative powertrains and fuels in the German transport sector. A model based scenario analysis with respect to technical, economic and environmental aspects with a focus on road transport

    International Nuclear Information System (INIS)

    The transport sector is facing the challenges of satisfying the ever increasing transport demand on the one hand and achieving greenhouse gas (GHG) emission reduction targets without compromising economic development on the other hand. There are various alternative fuels and powertrains which might play a role in the future of the German transport sector. Amongst these options, biofuels are considered to help lower GHG emissions. However, they are severely criticized to create an additional strain for the energy system and particularly for the transport sector with land area requirement for energy crop production, which may imply a competition with food production. This study aims to assess the future role of alternative fuels and powertrains in the German transport sector in terms of their costs, efficiencies, GHG emissions and land area requirement for energy crops. To fulfill this aim, a techno-economic analysis of all relevant fuels and powertrain options was performed and a model based approach was employed. The utilized model belongs to the TIMES (The Integrated MARKAL EFOM System) family and is a bottom-up linear cost optimization energy system model. A scenario analysis was employed in order to assess the effect of different technological, economic, environmental and political conditions on the overall system. The results of the scenario analysis indicated that the transport system will still be dominated by conventional powertrains in 2030. Alternative powertrains are projected to play only a secondary role until 2030. It is not expected that fuel cell or battery electric passenger cars will be introduced into the market until 2030 in Germany. Nevertheless, hybrid electric powertrains have to be used in the German passenger car sector under ambitious GHG emission reduction targets and high oil prices. The introduction of alternative powertrains (such as hybrid electric and fuel cell powertrain) is much more likely in the bus sector (especially for public

  20. Alternative and Integrative Medicine

    Science.gov (United States)

    ... are the healthcare rituals practiced by a given culture (eg, Asian, Indian, African). Homeopathic Medicine: This alternative medicine system is based on the principle that “like cures like.” In other words, the same substance ... American Brain Tumor Association 8550 W. Bryn Mawr Ave. ...

  1. Alternative Energy Busing

    Science.gov (United States)

    LaFee, Scott

    2012-01-01

    In recent years, school districts have converted portions of their bus fleets to cleaner-burning, sometimes cheaper, alternative fossil fuels, such as compressed natural gas or propane. Others have adopted biodiesel, which combines regular diesel with fuel derived from organic sources, usually vegetable oils or animal fats. The number of biodiesel…

  2. Compensated pulsed alternator

    International Nuclear Information System (INIS)

    This invention relates to an electromechanical energy converter with inertial energy storage. The device, a single phase, two or multi-pole alternator with stationary field coils, and a rotating armature is provided. The rotor itself may be of laminated steel for slower pulses or for faster pulses should be nonmagnetic and electrically nonconductive in order to allow rapid penetration of the field as the armature coil rotates. The armature coil comprises a plurality of power generating conductors mounted on the rotor. The alternator may also include a stationary or counterrotating compensating coil to increase the output voltage thereof and to reduce the internal impedance of the alternator at the moment of peak output. As the machine voltage rises sinusoidally, an external trigger switch is adapted to be closed at the appropriate time to create the desired output current from said alternator to an external load circuit, and as the output current passes through zero a self-commutating effect is provided to allow the switch to disconnect the generator from the external circuit

  3. Alternatives to Violence.

    Science.gov (United States)

    Children Today, 1994

    1994-01-01

    Notes that our capacity to diffuse conflict rests in our ability to recognize and verbalize feelings, develop empathy, and think of alternatives to violence. Explores the influence of role models and culture on violence and how the media can use violent images effectively in helping us confront a culture of violence. (HTH)

  4. Alternatives to Traditional Notation.

    Science.gov (United States)

    Gaare, Mark

    1997-01-01

    Provides a introduction and overview to alternative music notation systems. Describes guitar tablature, accordion tablature, klavarskribo (a keyboard notational system developed by Cornelius Pot, a Dutch engineer), and the digital piano roll. Briefly discusses the history of notation reform and current efforts. Includes examples from scores. (MJP)

  5. Energy conversion alternatives study

    Science.gov (United States)

    Shure, L. T.

    1979-01-01

    Comparison of coal based energy systems is given. Study identifies and compares various advanced energy conversion systems using coal or coal derived fuels for baselaoad electric power generation. Energy Conversion Alternatives Study (ECAS) reports provede government, industry, and general public with technically consistent basis for comparison of system's options of interest for fossilfired electric-utility application.

  6. Alternatives in solar energy

    Science.gov (United States)

    Schueler, D. G.

    1978-01-01

    Although solar energy has the potential of providing a significant source of clean and renewable energy for a variety of applications, it is expected to penetrate the nation's energy economy very slowly. The alternative solar energy technologies which employ direct collection and conversion of solar radiation as briefly described.

  7. A multicenter analysis of the use of complementary and alternative medicine in Turkish patients with rheumatoid arthritis: holistic nursing practice review copy.

    Science.gov (United States)

    Tokem, Yasemin; Parlar Kilic, Serap; Ozer, Serap; Nakas, Dilek; Argon, Gulumser

    2014-01-01

    The purpose of this study was to examine the complementary and alternative medicine (CAM) use in patients with rheumatoid arthritis living in different regions and cities of Turkey as well as the factors affecting the use of CAM modalities. Planned as a descriptive and cross-sectional study, this research was conducted as a study covering the rheumatology units of 10 university and 4 state hospitals in Turkey. A total of 594 patients meeting the research inclusion criteria comprised the sample. It was detected that 46.9% (n = 279) of the patients with rheumatoid arthritis whose average disease duration was 10.32 ± 9.05 years used CAM modalities. The most common 3 modalities used by these patients were herbs taken orally, nutritional supplements, and mind-body therapies, with rates of 54.5%, 41.2%, and 40.5%, respectively. It was determined that such variables as age, sex, marital status, education status, and economic situation did not affect the use of CAM (P > .05). PMID:24503747

  8. Analysis of the strengthenings of mixed glass fibers E and fibers AR in plaster, as an alternative to monofiber (homogeneous strengthenings

    Directory of Open Access Journals (Sweden)

    del Río Merino, M.

    2002-12-01

    Full Text Available The company Vetrotex in collaboration with the Department of Architectural Constructions and their control (E.U.A.T of the Polytechnic University of Madrid, have deeply researched on plaster strengthened with glass fiber E. The conclusions of the influence of the dispersibility of glass fiber in the compound mechanical behavior and in its workability have been published in an earlier article. Here now are the results and conclusions of a study on mixed strengthenings of glass fibers E and AR combined at a 50%, as an alternative to the homogenous strengthening.

    La empresa Vetrotex, a través de sus técnicos y en colaboración con el Departamento de Construcciones Arquitectónicas y su control (E.U.A.T de la UPM, decide acometer un estudio en profundidad de la escayola reforzada con fibra de vidrio E. En un primer artículo se presentaron las conclusiones sobre la influencia del grado de dispersabilidad de las fibras de vidrio en el comportamiento mecánico del compuesto y en su trabajabilidad. En este segundo artículo se presentan los resultados y conclusiones del estudio de los refuerzos mixtos de fibras de vidrio E en combinación al 50% con fibras de vidrio AR, como alteruativa a los refuerzos actuales homogéneos.

  9. A tiered approach to the use of alternatives to animal testing for the safety assessment of cosmetics: genotoxicity. A COLIPA analysis.

    Science.gov (United States)

    Pfuhler, Stefan; Kirst, Annette; Aardema, Marilyn; Banduhn, Norbert; Goebel, Carsten; Araki, Daisuke; Costabel-Farkas, Margit; Dufour, Eric; Fautz, Rolf; Harvey, James; Hewitt, Nicola J; Hibatallah, Jalila; Carmichael, Paul; Macfarlane, Martin; Reisinger, Kerstin; Rowland, Joanna; Schellauf, Florian; Schepky, Andreas; Scheel, Julia

    2010-01-01

    For the assessment of genotoxic effects of cosmetic ingredients, a number of well-established and regulatory accepted in vitro assays are in place. A caveat to the use of these assays is their relatively low specificity and high rate of false or misleading positive results. Due to the 7th amendment to the EU Cosmetics Directive ban on in vivo genotoxicity testing for cosmetics that was enacted March 2009, it is no longer possible to conduct follow-up in vivo genotoxicity tests for cosmetic ingredients positive in in vitro genotoxicity tests to further assess the relevance of the in vitro findings. COLIPA, the European Cosmetics Association, has initiated a research programme to improve existing and develop new in vitro methods. A COLIPA workshop was held in Brussels in April 2008 to analyse the best possible use of available methods and approaches to enable a sound assessment of the genotoxic hazard of cosmetic ingredients. Common approaches of cosmetic companies are described, with recommendations for evaluating in vitro genotoxins using non-animal approaches. A weight of evidence approach was employed to set up a decision-tree for the integration of alternative methods into tiered testing strategies. PMID:20382194

  10. Inverse PCR and Quantitative PCR as Alternative Methods to Southern Blotting Analysis to Assess Transgene Copy Number and Characterize the Integration Site in Transgenic Woody Plants.

    Science.gov (United States)

    Stefano, Biricolti; Patrizia, Bogani; Matteo, Cerboneschi; Massimo, Gori

    2016-06-01

    One of the major unanswered questions with respect to the commercial use of genetic transformation in woody plants is the stability of the transgene expression over several decades within the same individual. Gene expression is strongly affected by the copy number which has been integrated into the plant genome and by the local DNA features close to the integration sites. Because woody plants cannot be subjected to selfing or backcrossing to modify the transgenic allelic structure without affecting the valuable traits of the cultivar, molecular characterization of the transformation event is therefore crucial. After assessing the transgene copy number of a set of apple transgenic clones with Southern blotting, we describe two alternative methods: the first is based on inverse PCR (i-PCR) and the second on the quantitative PCR (q-PCR). The methods produced comparable results with the exception of the data regarding a high copy number clone, but while the q-PCR-based system is rapid and easily adaptable to high throughput systems, the i-PCR-based method can provide information regarding the transformation event and the characteristics of the sequences flanking the transgenic construct.

  11. Computational modeling as part of alternative testing strategies in the respiratory and cardiovascular systems: inhaled nanoparticle dose modeling based on representative aerosol measurements and corresponding toxicological analysis.

    Science.gov (United States)

    Pilou, Marika; Mavrofrydi, Olga; Housiadas, Christos; Eleftheriadis, Kostas; Papazafiri, Panagiota

    2015-05-01

    The objectives of modeling in this work were (a) the integration of two existing numerical models in order to connect external exposure to nanoparticles (NPs) with internal dose through inhalation, and (b) to use computational fluid-particle dynamics (CFPD) to analyze the behavior of NPs in the respiratory and the cardiovascular system. Regarding the first objective, a lung transport and deposition model was combined with a lung clearance/retention model to estimate NPs dose in the different regions of the human respiratory tract and some adjacent tissues. On the other hand, CFPD was used to estimate particle transport and deposition of particles in a physiologically based bifurcation created by the third and fourth lung generations (respiratory system), as well as to predict the fate of super-paramagnetic particles suspended in a liquid under the influence of an external magnetic field (cardiovascular system). All the above studies showed that, with proper refinement, the developed computational models and methodologies may serve as an alternative testing strategy, replacing transport/deposition experiments that are expensive both in time and resources and contribute to risk assessment.

  12. Alternative fuels: a Brazilian outlook

    International Nuclear Information System (INIS)

    This paper focuses on studies and information related to the use of alternative fuels in Brazil. The first part of this paper deals with the economics of different biomass technologies. The analysis consists of a careful costing of all operations involved. The study deals with wood, sugar cane and cassava, since these crops are exploited for commercial purposes in Brazil. Corn, although a useful raw material for producing ethanol in the United States, is not used for this purpose in Brazil. The second part deals with the industrial technologies used to convert biomass into energy. We consider several forms of energy derived from biomass and evaluate the economics of the processes. When opportune, we compare costs with those of the North American market. Market analysis and displacement of conventional energy are the subject of the third part of the paper. While the cost of each product is evaluated in most cases; in others the current market price is used. Finally, we raise the issues of institutional problems and planning and offer some conclusions on the future of biomass as an alternative energy source. The technological discussion in this paper is based on the Brazilian experience in producing ethanol and other fuels from biomass. It is possible to extrapolate the Brazilian experience to other developing countries. The observations made in this chapter are based on the conditions prevalent in the Brazilian south-central agricultural region, specifically the state of Sao Paulo. (author). 91 refs., 16 figs., 11 tabs

  13. Development of alternative data analysis techniques for improving the accuracy and specificity of natural resource inventories made with digital remote sensing data

    Science.gov (United States)

    Lillesand, T. M.; Meisner, D. E. (Principal Investigator)

    1980-01-01

    An investigation was conducted into ways to improve the involvement of state and local user personnel in the digital image analysis process by isolating those elements of the analysis process which require extensive involvement by field personnel and providing means for performing those activities apart from a computer facility. In this way, the analysis procedure can be converted from a centralized activity focused on a computer facility to a distributed activity in which users can interact with the data at the field office level or in the field itself. A general image processing software was developed on the University of Minnesota computer system (Control Data Cyber models 172 and 74). The use of color hardcopy image data as a primary medium in supervised training procedures was investigated and digital display equipment and a coordinate digitizer were procured.

  14. Cost-benefit analysis for waste compaction alternatives at Lawrence Livermore National Laboratory: Addendum A to the Waste Minimization and Pollution Prevention Awareness Plan of May 31, 1991

    International Nuclear Information System (INIS)

    This report presents a cost-benefit analysis of the potential procurement and operation of various solid waste compactors or of the use of commercial compaction services, for compaction of solid transuranic (TRU), low-level radioactive, hazardous, and mixed wastes at Lawrence Livermore National Laboratory (LLNL) Hazardous Waste Management (HWM) facilities. The cost-benefit analysis was conducted to determine if increased compaction capacity at HWM might afford the potential for significant waste volume reduction and annual savings in material, shipping, labor, and disposal costs

  15. Allogeneic hematopoietic stem cell transplantation from an alternative stem cell source in Fanconi anemia patients: analysis of 47 patients from a single institution

    Directory of Open Access Journals (Sweden)

    C.R. de Medeiros

    2006-10-01

    Full Text Available We transplanted 47 patients with Fanconi anemia using an alternative source of hematopoietic cells. The patients were assigned to the following groups: group 1, unrelated bone marrow (N = 15; group 2, unrelated cord blood (N = 17, and group 3, related non-sibling bone marrow (N = 15. Twenty-four patients (51% had complete engraftment, which was not influenced by gender (P = 0.87, age (P = 0.45, dose of cyclophosphamide (P = 0.80, nucleated cell dose infused (P = 0.60, or use of anti-T serotherapy (P = 0.20. Favorable factors for superior engraftment were full HLA compatibility (independent of the source of cells; P = 0.007 and use of a fludarabine-based conditioning regimen (P = 0.046. Unfavorable factors were > or = 25 transfusions pre-transplant (P = 0.011 and degree of HLA disparity (P = 0.007. Intensity of mucositis (P = 0.50 and use of androgen prior to transplant had no influence on survival (P = 0.80. Acute graft-versus-host disease (GVHD grade II-IV and chronic GVHD were diagnosed in 47 and 23% of available patients, respectively, and infections prevailed as the main cause of death, associated or not with GVHD. Eighteen patients are alive, the Kaplan-Meyer overall survival is 38% at ~8 years, and the best results were obtained with related non-sibling bone marrow patients. Three recommendations emerged from the present study: fludarabine as part of conditioning, transplant in patients with <25 transfusions and avoidance of HLA disparity. In addition, an extended family search (even when consanguinity is not present seeking for a related non-sibling donor is highly recommended.

  16. Quantitative analysis of the effect of zidovudine, efavirenz, and ritonavir on insulin aggregation by multivariate curve resolution alternating least squares of infrared spectra

    International Nuclear Information System (INIS)

    Highlights: ► The structure of insulin can be changed via interaction with antiretroviral drugs. ► The chemical interaction promotes the formation of aggregates. ► This drug effect was evaluated by MCR-ALS coupled to IR spectroscopy. ► Formation of aggregates was favourable if drugs were able to form hydrogen bonds. ► Higher drug concentrations favoured formation of amorphous aggregates. - Abstract: Quantification of the effect of antiretroviral drugs on the insulin aggregation process is an important area of research due to the serious metabolic diseases observed in AIDS patients after prolonged treatment with these drugs. In this work, multivariate curve resolution alternating least squares (MCR-ALS) was applied to infrared monitoring of the insulin aggregation process in the presence of three antiretroviral drugs to quantify their effect. To evidence concentration dependence in this process, mixtures at two different insulin:drug molar ratios were used. The interaction between insulin and each drug was analysed by 1H NMR spectroscopy. In all cases, the aggregation process was monitored during 45 min by infrared spectroscopy. The aggregates were further characterised by scanning electron microscopy (SEM). MCR-ALS provided the spectral and concentration profiles of the different insulin–drug conformations that are involved in the process. Their feasible band boundaries were calculated using the MCR-BANDS methodology. The kinetic profiles describe the aggregation pathway and the spectral profiles characterise the conformations involved. The retrieved results show that each of the three drugs modifies insulin conformation in a different way, promoting the formation of aggregates. Ritonavir shows the strongest promotion of aggregation, followed by efavirenz and zidovudine. In the studied concentration range, concentration dependence was only observed for zidovudine, with shorter aggregation time obtained as the amount of zidovudine increased. This factor

  17. Quantitative analysis of the effect of zidovudine, efavirenz, and ritonavir on insulin aggregation by multivariate curve resolution alternating least squares of infrared spectra

    Energy Technology Data Exchange (ETDEWEB)

    Marti-Aluja, Idoia; Ruisanchez, Itziar [Analytical and Organic Chemistry Department, Universitat Rovira i Virgili, Marcelli Domingo s/n, Campus Sescelades, 43007 Tarragona (Spain); Larrechi, M. Soledad, E-mail: mariasoledad.larrechi@urv.cat [Analytical and Organic Chemistry Department, Universitat Rovira i Virgili, Marcelli Domingo s/n, Campus Sescelades, 43007 Tarragona (Spain)

    2013-01-14

    Highlights: Black-Right-Pointing-Pointer The structure of insulin can be changed via interaction with antiretroviral drugs. Black-Right-Pointing-Pointer The chemical interaction promotes the formation of aggregates. Black-Right-Pointing-Pointer This drug effect was evaluated by MCR-ALS coupled to IR spectroscopy. Black-Right-Pointing-Pointer Formation of aggregates was favourable if drugs were able to form hydrogen bonds. Black-Right-Pointing-Pointer Higher drug concentrations favoured formation of amorphous aggregates. - Abstract: Quantification of the effect of antiretroviral drugs on the insulin aggregation process is an important area of research due to the serious metabolic diseases observed in AIDS patients after prolonged treatment with these drugs. In this work, multivariate curve resolution alternating least squares (MCR-ALS) was applied to infrared monitoring of the insulin aggregation process in the presence of three antiretroviral drugs to quantify their effect. To evidence concentration dependence in this process, mixtures at two different insulin:drug molar ratios were used. The interaction between insulin and each drug was analysed by {sup 1}H NMR spectroscopy. In all cases, the aggregation process was monitored during 45 min by infrared spectroscopy. The aggregates were further characterised by scanning electron microscopy (SEM). MCR-ALS provided the spectral and concentration profiles of the different insulin-drug conformations that are involved in the process. Their feasible band boundaries were calculated using the MCR-BANDS methodology. The kinetic profiles describe the aggregation pathway and the spectral profiles characterise the conformations involved. The retrieved results show that each of the three drugs modifies insulin conformation in a different way, promoting the formation of aggregates. Ritonavir shows the strongest promotion of aggregation, followed by efavirenz and zidovudine. In the studied concentration range, concentration

  18. Adaptive Alternating Minimization Algorithms

    CERN Document Server

    Niesen, Urs; Wornell, Gregory

    2007-01-01

    The classical alternating minimization (or projection) algorithm has been successful in the context of solving optimization problems over two variables or equivalently of finding a point in the intersection of two sets. The iterative nature and simplicity of the algorithm has led to its application to many areas such as signal processing, information theory, control, and finance. A general set of sufficient conditions for the convergence and correctness of the algorithm is quite well-known when the underlying problem parameters are fixed. In many practical situations, however, the underlying problem parameters are changing over time, and the use of an adaptive algorithm is more appropriate. In this paper, we study such an adaptive version of the alternating minimization algorithm. As a main result of this paper, we provide a general set of sufficient conditions for the convergence and correctness of the adaptive algorithm. Perhaps surprisingly, these conditions seem to be the minimal ones one would expect in ...

  19. Comparison of Alternative Processes for Support Decisions

    Directory of Open Access Journals (Sweden)

    Manuel Martínez-Álvarez

    2014-08-01

    Full Text Available There are many tasks that revolve around combinatorial analysis problems, same tasks found in Decision Support Systems (DSS as most of these are responsible for assessing a number of possibilities to deliver the best options. Within the analysis of possible solutions is performed by the DSS there are alternative procedures inside the engine for making decisions that involve them. As part of these alternative procedures today has highlighted the use of metaheuristics, thus in this paper we propose a comparison of some of them trying to broaden the spectrum we have for the applications nowadays.

  20. Alternatives to Higgs scalars

    International Nuclear Information System (INIS)

    We discuss alternatives to the Higgs Scalar doublet Φ of the minimal standard model. In particular we review technicolor (TC) and extended technicolor (ETC), and show that the threat of flavour changing neutral currents (FCNC's) is avoided by walking TC and/or strongly coupled ETC. The phenomenology of minimal TC and the techni-family is discussed. Finally we consider the possibility that the Higgs doublet Φ is a composite bound state of quarks from the third (or fourth) family

  1. Alternatives to ECMO.

    OpenAIRE

    Donn, S M

    1994-01-01

    The past decade has witnessed technological advancements which are unparalleled in neonatology. ECMO has been demonstrated to be a powerful rescue treatment, but has perhaps been overutilised and is not universally available. Alternative treatments have been shown to be both safe and efficacious in the management of infants with respiratory failure. Direct head to head clinical trials will probably be necessary to establish appropriate criteria and indications for use, given the wide diversit...

  2. Alternative approaches in IVF

    OpenAIRE

    Fauser, Bart; Bouchard, P.; Coelingh Bennink, H.J.; Collins, John; Devroey, P.; Evers, Johannes; Van Steirteghem, A

    2002-01-01

    textabstractVarious new developments in clinical and basic science which may impact on IVF in the near or distant future will be discussed in this review. These key areas include the regulation of early follicle development and the extended in-vitro culture of oocytes and embryos. Moreover, alternative compounds and ovarian stimulation protocols will be discussed, along with highlights in the development of the cryopreservation of excess oocytes or embryos. Finally, the health economics of IV...

  3. Metamaterials critique and alternatives

    CERN Document Server

    Munk, Ben A

    2009-01-01

    A Convincing and Controversial Alternative Explanation of Metamaterials with a Negative Index of Refraction In a book that will generate both support and controversy, one of the world's foremost authorities on periodic structures addresses several of the current fashions in antenna design-most specifically, the popular subject of double negative metamaterials. Professor Munk provides a comprehensive theoretical electromagnetic investigation of the issues and concludes that many of the phenomena claimed by researchers may be impossible. While denying the existence of negative refractio

  4. Outlook for alternative transportation fuels

    Energy Technology Data Exchange (ETDEWEB)

    Gushee, D.E. [Univ. of Illinois, Chicago, IL (United States)

    1996-12-31

    This presentation provides a brief review of regulatory issues and Federal programs regarding alternative fuel use in automobiles. A number of U.S. DOE initiatives and studies aimed at increasing alternative fuels are outlined, and tax incentives in effect at the state and Federal levels are discussed. Data on alternative fuel consumption and alternative fuel vehicle use are also presented. Despite mandates, tax incentives, and programs, it is concluded alternative fuels will have minimal market penetration. 7 refs., 5 tabs.

  5. Alternative Energy Sources

    CERN Document Server

    Michaelides, Efstathios E (Stathis)

    2012-01-01

    Alternative Energy Sources is designed to give the reader, a clear view of the role each form of alternative energy may play in supplying the energy needs of the human society in the near and intermediate future (20-50 years).   The two first chapters on energy demand and supply and environmental effects, set the tone as to why the widespread use of alternative energy is essential for the future of human society. The third chapter exposes the reader to the laws of energy conversion processes, as well as the limitations of converting one energy form to another. The sections on exergy give a succinct, quantitative background on the capability/potential of each energy source to produce power on a global scale. The fourth, fifth and sixth chapters are expositions of fission and fusion nuclear energy. The following five chapters (seventh to eleventh) include detailed descriptions of the most common renewable energy sources – wind, solar, geothermal, biomass, hydroelectric – and some of the less common sources...

  6. Alternative Fuels: Research Progress

    Directory of Open Access Journals (Sweden)

    Maher A.R. Sadiq Al-Baghdadi

    2013-01-01

    Full Text Available Chapter 1: Pollutant Emissions and Combustion Characteristics of Biofuels and Biofuel/Diesel Blends in Laminar and Turbulent Gas Jet Flames. R. N. Parthasarathy, S. R. Gollahalli Chapter 2: Sustainable Routes for The Production of Oxygenated High-Energy Density Biofuels from Lignocellulosic Biomass. Juan A. Melero, Jose Iglesias, Gabriel Morales, Marta Paniagua Chapter 3: Optical Investigations of Alternative-Fuel Combustion in an HSDI Diesel Engine. T. Huelser, M. Jakob, G. Gruenefeld, P. Adomeit, S. Pischinger Chapter 4: An Insight into Biodiesel Physico-Chemical Properties and Exhaust Emissions Based on Statistical Elaboration of Experimental Data. Evangelos G. Giakoumis Chapter 5: Biodiesel: A Promising Alternative Energy Resource. A.E. Atabani Chapter 6: Alternative Fuels for Internal Combustion Engines: An Overview of the Current Research. Ahmed A. Taha, Tarek M. Abdel-Salam, Madhu Vellakal Chapter 7: Investigating the Hydrogen-Natural Gas Blends as a Fuel in Internal Combustion Engine. ?lker YILMAZ Chapter 8: Conversion of Bus Diesel Engine into LPG Gaseous Engine; Method and Experiments Validation. M. A. Jemni , G. Kantchev , Z. Driss , R. Saaidia , M. S. Abid Chapter 9: Predicting the Combustion Performance of Different Vegetable Oils-Derived Biodiesel Fuels. Qing Shu, ChangLin Yu Chapter 10: Production of Gasoline, Naphtha, Kerosene, Diesel, and Fuel Oil Range Fuels from Polypropylene and Polystyrene Waste Plastics Mixture by Two-Stage Catalytic Degradation using ZnO. Moinuddin Sarker, Mohammad Mamunor Rashid

  7. An alternative method to amplify RNA without loss of signal conservation for expression analysis with a proteinase DNA microarray in the ArrayTube® format

    Directory of Open Access Journals (Sweden)

    Wiederanders B

    2006-06-01

    RNA starts from as little as 100 ng total RNA, it presents an alternative method for detecting even low expressed genes by microarray experiments in a highly reproducible and sensitive manner. Preservation of signal integrity is demonstrated out by QRT-PCR measurements. The little amounts of total RNA necessary for the analyses make this method applicable for investigations with limited material as in clinical samples from, for example, organ or tumour biopsies. Those are arguments in favour of the high potential of our assay compared to established procedures for amplification within the field of diagnostic expression profiling. Nevertheless, the screening character of microarray data must be mentioned, and independent methods should verify the results.

  8. Personalized versus non-personalized computerized decision support system to increase therapeutic quality control of oral anticoagulant therapy: an alternating time series analysis

    Directory of Open Access Journals (Sweden)

    Chatellier Gilles

    2004-09-01

    Full Text Available Abstract Background The quality control of oral anticoagulant therapy (OAT during the initiation and maintenance treatment is generally poor. Physicians' ordering of OAT (especially fluindione and warfarin can be improved by dose adjustment algorithms, taking into account the results of International Normalized Ratio (INR. Reminders at the point of care, computerized or not, have been demonstrated to be effective in changing physicians prescription behavior. However, few studies have addressed the benefit of personalized reminders versus non personalized reminders, whereas the personalized reminders require more development to access patient record data and integrate with the computerized physician order entry system. The Hospital Information System of George Pompidou European Hospital integrates an electronic medical record, lab test and drugs order entry system. This system allows to evaluate such reminders and to consider their implementation for routine use as well as the continuous evaluation of their impact on medical practice quality indicators. The objective of this study is to evaluate the impact of two types of reminders on overtreatment by oral anticoagulant: a simple reminder of text formatted dose adjustment table and a personalized recommendation for oral anticoagulant dose and next date of INR control, adapted to patient data. Both types of reminders appear to the physician at the moment of drug ordering. Methods The study is an alternating time series experiment with three 6 months periods, each one including every 2 months according to a Latin square scheme: a control period without any reminder, a period with the simple non personalized reminder, a period with personalized reminder. All patients hospitalized in departments using the computerized physician order entry system and ordered fluindione or warfarin, will be included in the study between November 2004 and May 2006. Main outcome will be the proportion of overcoagulation

  9. Análise econômica de esquemas alternativos de controle leiteiro Economic analysis of alternative schemes of milk recording

    Directory of Open Access Journals (Sweden)

    V.L. Cardoso

    2005-02-01

    Full Text Available Compararam-se os custos de seis esquemas de controle leiteiro (CL, estabelecidos com base em diferentes freqüências de controles (com possibilidade de controle trimestral supervisionado e aproveitamento do CL da fazenda, no número de ordenhas e na realização de análises qualitativas (composição do leite e/ou contagem de células somáticas. Computaram-se os percentuais que os custos totais do CL representariam na receita bruta mensal do leite (CL/RB e na receita líquida mensal (CL/RL. O esquema tradicional (freqüência mensal apresentou o mais elevado custo mensal e os maiores CL/RB e CL/RL. Esquemas com intervalo maior entre controles e amostragem em ordenhas alternadas resultaram na redução dos custos com diárias e quilometragem. O custo mensal do CL variou de 0,68% a 1,8% sobre a receita bruta do leite e de 6,6% a 17,0% sobre a receita líquida.Costs of six different milk recording schemes based on the interval between controls (with the possibility of a three-month interval supervision control and the use of the farmers’ milk recording data, the number of milking recorded and milk quality analyses (milk composition and/or somatic cell counting were compared. The ratio milk recording costs over gross income (CL/RB and over net income (CL/RL were also estimated. The traditional scheme (monthly recording presented the highest monthly costs and ratios CL/RB and CL/RL. Schemes with longer intervals between controls and alternate sampling presented the lowest costs due to the reduction of travel costs. Monthly milk recording costs over milk gross income and over net income ranged from 0.68% to 1.8% and from 6.6% to 17.0%, respectively.

  10. Alternative Energy Development and China's Energy Future

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Nina; Fridley, David

    2011-06-15

    In addition to promoting energy efficiency, China has actively pursued alternative energy development as a strategy to reduce its energy demand and carbon emissions. One area of particular focus has been to raise the share of alternative energy in China’s rapidly growing electricity generation with a 2020 target of 15% share of total primary energy. Over the last ten years, China has established several major renewable energy regulations along with programs and subsidies to encourage the growth of non-fossil alternative energy including solar, wind, nuclear, hydro, geothermal and biomass power as well as biofuels and coal alternatives. This study thus seeks to examine China’s alternative energy in terms of what has and will continue to drive alternative energy development in China as well as analyze in depth the growth potential and challenges facing each specific technology. This study found that despite recent policies enabling extraordinary capacity and investment growth, alternative energy technologies face constraints and barriers to growth. For relatively new technologies that have not achieved commercialization such as concentrated solar thermal, geothermal and biomass power, China faces technological limitations to expanding the scale of installed capacity. While some alternative technologies such as hydropower and coal alternatives have been slowed by uneven and often changing market and policy support, others such as wind and solar PV have encountered physical and institutional barriers to grid integration. Lastly, all alternative energy technologies face constraints in human resources and raw material resources including land and water, with some facing supply limitations in critical elements such as uranium for nuclear, neodymium for wind and rare earth metals for advanced solar PV. In light of China’s potential for and barriers to growth, the resource and energy requirement for alternative energy technologies were modeled and scenario analysis

  11. Modeling corrosion inhibition efficacy of small organic molecules as non-toxic chromate alternatives using comparative molecular surface analysis (CoMSA).

    Science.gov (United States)

    Fernandez, Michael; Breedon, Michael; Cole, Ivan S; Barnard, Amanda S

    2016-10-01

    Traditionally many structural alloys are protected by primer coatings loaded with corrosion inhibiting additives. Strontium Chromate (or other chromates) have been shown to be extremely effectively inhibitors, and find extensive use in protective primer formulations. Unfortunately, hexavalent chromium which imbues these coatings with their corrosion inhibiting properties is also highly toxic, and their use is being increasingly restricted by legislation. In this work we explore a novel tridimensional Quantitative-Structure Property Relationship (3D-QSPR) approach, comparative molecular surface analysis (CoMSA), which was developed to recognize "high-performing" corrosion inhibitor candidates from the distributions of electronegativity, polarizability and van der Waals volume on the molecular surfaces of 28 small organic molecules. Multivariate statistical analysis identified five prototypes molecules, which are capable of explaining 71% of the variance within the inhibitor data set; whilst a further five molecules were also identified as archetypes, describing 75% of data variance. All active corrosion inhibitors, at a 80% threshold, were successfully recognized by the CoMSA model with adequate specificity and precision higher than 70% and 60%, respectively. The model was also capable of identifying structural patterns, that revealed reasonable starting points for where structural changes may augment corrosion inhibition efficacy. The presented methodology can be applied to other functional molecules and extended to cover structure-activity studies in a diverse range of areas such as drug design and novel material discovery. PMID:27362530

  12. USACE FUSRAP Maywood Team Identifies Challenges and Initiates Alternate Solutions Relating to the Radiochemical Analysis of Borosilicate Fiber Filters for Isotopes of Uranium and Thorium

    International Nuclear Information System (INIS)

    This presentation discusses the primary purposes of particulate radionuclide air monitoring at the US Army Corps of Engineers (USACE) Formerly Utilized Sites Remediation Program (FUSRAP) Maywood Superfund Site (FMSS), the challenges encountered by the team when standard radiochemistry analytical methods are attempted on borosilicate fiber filter samples, the surrogate evaluations used when sample specific isotopic analysis is unsuccessful, and current strategies for overcoming radiochemistry method deficiencies. Typical borosilicate fiber filter sample preparation procedures including tracer spike and digestion methods and their impact on uranium and thorium data quality are of particular interest. Analytes discussed include isotopic uranium (U-234, U-235, and U-238) and isotopic thorium (Th-228, Th-230, and Th-232). Efforts to obtain reproducible and defensible results also included discussions with commercial laboratory radiochemistry managers as well as industry experts. This presentation may benefit sites that use similar sample collection and analysis techniques, utilize data that may have unidentified method-related issues with diminished data quality, or have a similar isotopic signature. (authors)

  13. Modeling corrosion inhibition efficacy of small organic molecules as non-toxic chromate alternatives using comparative molecular surface analysis (CoMSA).

    Science.gov (United States)

    Fernandez, Michael; Breedon, Michael; Cole, Ivan S; Barnard, Amanda S

    2016-10-01

    Traditionally many structural alloys are protected by primer coatings loaded with corrosion inhibiting additives. Strontium Chromate (or other chromates) have been shown to be extremely effectively inhibitors, and find extensive use in protective primer formulations. Unfortunately, hexavalent chromium which imbues these coatings with their corrosion inhibiting properties is also highly toxic, and their use is being increasingly restricted by legislation. In this work we explore a novel tridimensional Quantitative-Structure Property Relationship (3D-QSPR) approach, comparative molecular surface analysis (CoMSA), which was developed to recognize "high-performing" corrosion inhibitor candidates from the distributions of electronegativity, polarizability and van der Waals volume on the molecular surfaces of 28 small organic molecules. Multivariate statistical analysis identified five prototypes molecules, which are capable of explaining 71% of the variance within the inhibitor data set; whilst a further five molecules were also identified as archetypes, describing 75% of data variance. All active corrosion inhibitors, at a 80% threshold, were successfully recognized by the CoMSA model with adequate specificity and precision higher than 70% and 60%, respectively. The model was also capable of identifying structural patterns, that revealed reasonable starting points for where structural changes may augment corrosion inhibition efficacy. The presented methodology can be applied to other functional molecules and extended to cover structure-activity studies in a diverse range of areas such as drug design and novel material discovery.

  14. Modified Waste Emplacement Mode Design Alternative Report

    International Nuclear Information System (INIS)

    emplacement mode. Calculations to determine seepage rates from degraded waste packages, and therefore exposure rates to the environment over time, were not generated. Since total system performance is highly dependent upon waste package performance, additional calculations are required to determine the complete results of this evaluation. The TSPA calculations that were generated for the alternative emplacement modes were then compared with the results of the TSPA-VA. The short cross-drift and the borehole emplacement modes are similar to the TSPA-VA Base Case, and therefore, the waste package failure rates and the total system performance are also expected to be similar. Calculations for TSPA for the in-trench emplacement modes were not generated. However, preliminary analysis by the Performance Assessment organization provides some initial evidence that the total system performance is expected to be similar to the TSPA-VA Base Case. Hence, the alternative emplacement modes considered in this report are not expected to rate higher in postclosure performance when compared to the VA reference design. The alternative emplacement modes rate below or equivalent to the VA reference design in preclosure performance, assurance of safety, engineering acceptance, construction, operations and maintenance, schedule, cost and environmental considerations. Based upon these ratings, none of the alternative emplacement mode concepts appear superior to the VA reference design. Among the alternative emplacement modes, short cross-drift, which is the most similar to the reference emplacement mode appeared to rank highest, followed by in-trench emplacement, and then borehole emplacement. Although postclosure performance calculations have not been completed, none of the alternative emplacement modes are expected to perform superior to the VA reference design. Alternative emplacement modes, however, provide greater benefit to the VA design in allowing human access in the emplacement drift areas

  15. Modified waste emplacement mode design alternative report

    International Nuclear Information System (INIS)

    emplacement mode. Calculations to determine seepage rates from degraded waste packages, and therefore exposure rates to the environment over time, were not generated. Since total system performance is highly dependent upon waste package performance, additional calculations are required to determine the complete results of this evaluation. The TSPA calculations that were generated for the alternative emplacement modes were then compared with the results of the TSPA-VA. The short cross-drift and the borehole emplacement modes are similar to the TSPA-VA Base Case, and therefore, the waste package failure rates and the total system performance are also expected to be similar. Calculations for TSPA for the in-trench emplacement modes were not generated. However, preliminary analysis by the Performance Assessment organization provides some initial evidence that the total system performance is expected to be similar to the TSPA-VA Base Case. Hence, the alternative emplacement modes considered in this report are not expected to rate higher in postclosure performance when compared to the VA reference design. The alternative emplacement modes rate below or equivalent to the VA reference design in preclosure performance, assurance of safety, engineering acceptance, construction, operations and maintenance, schedule, cost and environmental considerations. Based upon these ratings, none of the alternative emplacement mode concepts appear superior to the VA reference design. Among the alternative emplacement modes, short cross-drift, which is the most similar to the reference emplacement mode appeared to rank highest, followed by in-trench emplacement, and then borehole emplacement. Although postclosure performance calculations have not been completed, none of the alternative emplacement modes are expected to perform superior to the VA reference design. Alternative emplacement modes, however, provide greater benefit to the VA design in allowing human access in the emplacement drift areas

  16. Alternate fuels; Combustibles alternos

    Energy Technology Data Exchange (ETDEWEB)

    Romero Paredes R, Hernando; Ambriz G, Juan Jose [Universidad Autonoma Metropolitana. Iztapalapa (Mexico)

    2003-07-01

    In the definition and description of alternate fuels we must center ourselves in those technological alternatives that allow to obtain compounds that differ from the traditional ones, in their forms to be obtained. In this article it is tried to give an overview of alternate fuels to the conventional derivatives of petroleum and that allow to have a clear idea on the tendencies of modern investigation and the technological developments that can be implemented in the short term. It is not pretended to include all the tendencies and developments of the present world, but those that can hit in a relatively short term, in accordance with agreed with the average life of conventional fuels. Nevertheless, most of the conversion principles are applicable to the spectrum of carbonaceous or cellulosic materials which are in nature, are cultivated or wastes of organic origin. Thus one will approach them in a successive way, the physical, chemical and biological conversions that can take place in a production process of an alternate fuel or the same direct use of the fuel such as burning the sweepings derived from the forests. [Spanish] En la definicion y descripcion de combustibles alternos nos debemos centrar en aquellas alternativas tecnologicas que permitan obtener compuestos que difieren de los tradicionales, al menos en sus formas de ser obtenidos. En este articulo se pretende dar un panorama de los combustibles alternos a los convencionales derivados del petroleo y que permita tener una idea clara sobre las tendencias de la investigacion moderna y los desarrollos tecnologicos que puedan ser implementados en el corto plazo. No se pretende abarcar todas las tendencias y desarrollos del mundo actual, sino aquellas que pueden impactar en un plazo relativamente corto, acordes con la vida media de los combustibles convencionales. Sin embargo, la mayor parte de los principios de conversion son aplicables al espectro de materiales carbonaceos o celulosicos los cuales se

  17. Alternate fusion concepts

    International Nuclear Information System (INIS)

    This review summarizes the status of alternate fusion concepts and plans for their future. The concepts selected for review are those employing electromagnetic confinement for which there have been reasonable predictions of net energy gain from pure fusion and which have shown significant recent development or are the subjects of ongoing international activity. They include advanced tokamaks, stellarators, the spherical torus, reversed-field pinch and dense z-field pinch devices, field reversed configuration, and spheromaks. In addition, an overall view of the status of each concept with respect to achieving ignition and to reactor designs is presented

  18. Alternate transportation system

    Science.gov (United States)

    Zertuche, Tony; Mckinnie, James

    1988-01-01

    Three missions have been identified by NASA for a Space Shuttle-supplementing Alternate Transportation System (ATS) encompassing combinations of booster vehicles, crew modules, and service modules: (1) to achieve manned access to orbit for Space Station crew rotation every 90 days, (2) the lofting of a logistics module resupplying the Space Station every 180 days, and (3) the simultaneous launch of both crews and logistics to the Space Station. A reentry glider is considered, in conjunction with the Space Shuttle's unmanned cargo version and the Apollo manned capsule, as an important ATS element. The Titan IV/NUS is used as a booster.

  19. Polyphase alternating codes

    OpenAIRE

    M. Markkanen; Vierinen, J.; Markkanen, J.

    2007-01-01

    We present a new class of alternating codes. Instead of the customary binary phase codes, the new codes utilize either p or p–1 phases, where p is a prime number. The first class of codes has code length pm, where m is a positive integer, the second class has code length p–1. We give an actual construction algorithm, and explain the principles behind it. We ...

  20. Polyphase alternating codes

    Directory of Open Access Journals (Sweden)

    M. Markkanen

    2008-08-01

    Full Text Available We present a new class of alternating codes. Instead of the customary binary phase codes, the new codes utilize either p or p–1 phases, where p is a prime number. The first class of codes has code length pm, where m is a positive integer, the second class has code length p–1. We give an actual construction algorithm, and explain the principles behind it. We handle a few specific examples in detail. The new codes offer an enlarged collection of code lengths for radar experiments.

  1. Systems level insights into alternate methane cycling modes in a freshwater lake via community transcriptomics, metabolomics and nano-SIMS analysis

    Energy Technology Data Exchange (ETDEWEB)

    Lidstrom, Mary E. [Univ. of Washington, Seattle, WA (United States); Chistoserdova, Ludmila [Univ. of Washington, Seattle, WA (United States); Kalyuzhnaya, Marina G. [Univ. of Washington, Seattle, WA (United States); Orphan, Victoria J. [California Inst. of Technology (CalTech), Pasadena, CA (United States); Beck, David A. [Univ. of Washington, Seattle, WA (United States)

    2014-08-07

    The research conducted as part of this project contributes significantly to the understanding of the microbes and their activities involved in methane metabolism in freshwater lake sediments and in the environment in a more global sense. Significant new insights have been gained into the identity of the species that are most active in methane oxidation. New concepts have been developed based on the new data on how these organisms metabolize methane, impacting not only environmental microbiology but also biotechnology, including biotechnology of next generation biofuels. Novel approaches have been developed for studying functional microbial communities, via holistic approaches, such as metagenomics, metatrancriptomics and metabolite analysis. As a result, a novel outlook has been obtained at how such communities operate in nature. Understanding methane-oxidizing communities in lakes and other environments is of significant benefit to the public, in terms of methane emission mitigation and in terms of potential biotechnological applications.

  2. Efficacy of once-daily indacaterol 75 μg relative to alternative bronchodilators in COPD: A study level and a patient level network meta-analysis

    Directory of Open Access Journals (Sweden)

    Cope Shannon

    2012-06-01

    Full Text Available Abstract Background The objective of this study was to evaluate the comparative efficacy of indacaterol 75 μg once daily (OD, tiotropium 18 μg OD, salmeterol 50 μg twice daily (BID, formoterol 12 μg BID, and placebo for the treatment of chronic obstructive pulmonary disease (COPD based on individual patient data (IPD from randomized controlled trials (RCTs from the indacaterol trial program and aggregate data (AD identified from a systematic review of RCTs. Methods 22 RCTs were included in the AD analysis that evaluated: indacaterol 75 μg (n = 2 studies, indacaterol 150 μg n = 5 (i.e. salmeterol 50 μg (n = 5, indacaterol 300 μg (n = 2, tiotropium 18 μg (n = 10, salmeterol 50 μg (n = 7, and formoterol 12 μg (n = 4. All of the studies except for one head-to-head comparison (tiotropium vs. salmeterol were placebo controlled. Outcomes of interest were trough forced expiratory volume in 1 second (FEV1 and St. George’s Respiratory Questionnaire (SGRQ total score at week 12. The AD from all trials was analysed simultaneously using a Bayesian network meta-analysis (NMA and relative treatment effects between all regimens were obtained. In a separate analysis, the IPD available from the 6 indacaterol RCTs was analysed in a NMA. Treatment-by-covariate interactions were included in both analyses to improve similarity of the trials. Results All interventions compared were more efficacious than placebo regarding FEV1 at 12 weeks. Indacaterol 75 μg is expected to result in a comparable FEV1 at 12 weeks to tiotropium and salmeterol based on both IPD and AD analyses. In comparison to formoterol, the IPD and AD results indicate indacaterol 75 μg is more efficacious (IPD = 0.07 L difference; 95%Credible Interval (CrI 0.02 to 0.11; AD = 0.05 L difference; 95%CrI 0.01; 0.09. In terms of SGRQ total score at 12 weeks, indacaterol 75 μg and formoterol were more efficacious than

  3. Nuclear energy: a sensible alternative

    International Nuclear Information System (INIS)

    This book presents information on energy futures; energy demand, energy supplies; exclusive paths and difficult choices--hard, soft, and moderate energy paths; an energy-deficient society; energy shortages; economics of light-water reactors; fast breeder reactor economics; international cooperation in the nuclear field; nuclear recycling; alternative fuels, fuel cycles, and reactors; the nuclear weapons proliferation issue; paths to a world with more reliable nuclear safeguards; the homemade bomb issue; LWR risk assessment; accident analysis and risk assessment; the waste disposal risk; radon problems; risks in our society; health effects of low-level radiation; routine releases of radioactivity from the nuclear industry; low-level radioactivity and infant mortality; the myth of plutonium toxicity; myths about high-level radioactive waste; the aging reactor myth; the police state myth; insurance and nuclear power--the Price-Anderson Act; and solar and nuclear power as partners

  4. A Note to Bollen's Alternative Fit Measure.

    Science.gov (United States)

    Balderjahn, Ingo

    1988-01-01

    The nonnormed fit index's dependence on sample size in covariance structure analysis is discussed. Contrary to K. A. Bollen (1986) (whose alternative index depends on sample size), it is shown that the mean of the nonnormed fit index is independent of sample size for true and almost true models. (Author/TJH)

  5. Cornerstone Third Party Alternative Cost Study

    Energy Technology Data Exchange (ETDEWEB)

    Vrieling, P. Douglas [Sandia National Lab. (SNL-CA), Livermore, CA (United States)

    2013-10-15

    The attached cost analyses sheets have been developed for use in planning during the Third Party Alternative study currently underway for the Sandia CREATE project. This cost analysis builds upon the previously submitted base estimate dated June 14, 2013 and includes comparison information collected during a Market Validation exercise conducted in August/September 2013.

  6. Research reactors and alternative devices for research

    International Nuclear Information System (INIS)

    This report includes papers on research reactors and alternatives to the research reactors - radioisotopic neutron sources, cyclotrons, D-T neutron generators and small accelerators, used for radioisotope production, neutron activation analysis, material science, applied and basic research using neutron beams. A separate abstract was prepared for each of the 7 papers

  7. Alternate Gauge Electroweak Model

    CERN Document Server

    Dalton, Bill

    2010-01-01

    We describe an alternate gauge electroweak model that permits neutrinos with mass, and at the same time explains why right-handed neutrinos do not appear in weak interactions. This is a local gauge theory involving a space [V ] of three scalar functions. The standard Lagrangian density for the Yang-Mills field part and Higgs doublet remain invariant. A ma jor change is made in the transformation and corresponding Lagrangian density parts involving the right-handed leptons. A picture involving two types of right-handed leptons emerges. A dichotomy of matter on the [V ] space corresponds to coupled and uncoupled right-handed Leptons. Here, we describe a covariant dipole-mode solution in which the neutral bosons A{\\mu} and Z{\\mu} produce precessions on [V ]. The W {\\pm} {\\mu} bosons provide nutations on [V ], and consequently, provide transitions between the coupled and uncoupled regions. To elucidate the [V ] space matter dichotomy, and to generate the boson masses, we also provide an alternate potential Lagran...

  8. Optimized immunohistochemistry in combination with image analysis: a reliable alternative to quantitative ELISA determination of uPA and PAI-1 for routine risk group discrimination in breast cancer.

    Science.gov (United States)

    Lang, D S; Heilenkötter, U; Schumm, W; Behrens, O; Simon, R; Vollmer, E; Goldmann, T

    2013-10-01

    The determination of the invasion markers urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) has further improved the possibilities for individualized therapy of breast cancer. To date, quantitative measurement by ELISA, that needs large amounts of fresh, frozen material, is the only standardized procedure for diagnostic purposes. Therefore, the aim of this study was the establishment of a reliable alternative method based on immunohistochemistry (IHC) and image analysis requiring only small amounts of fixed tumor tissue. Protein expression of uPA and PAI-1 was analyzed in HOPE-fixed tumor samples using tissue microarrays (TMAs) and semiquantitative image analysis. The results of both methods were significantly correlated and risk assessment showed an overall concordance of 78% (83/107; high- and low-risk) and of 94% (74/79) regarding only high-risk patients. The data demonstrate that optimized IHC in combination with image analysis can provide adequate clinical significance compared to ELISA-derived determination of uPA and PAI-1.

  9. Quantifying asymmetry: ratios and alternatives.

    Science.gov (United States)

    Franks, Erin M; Cabo, Luis L

    2014-08-01

    Traditionally, the study of metric skeletal asymmetry has relied largely on univariate analyses, utilizing ratio transformations when the goal is comparing asymmetries in skeletal elements or populations of dissimilar dimensions. Under this approach, raw asymmetries are divided by a size marker, such as a bilateral average, in an attempt to produce size-free asymmetry indices. Henceforth, this will be referred to as "controlling for size" (see Smith: Curr Anthropol 46 (2005) 249-273). Ratios obtained in this manner often require further transformations to interpret the meaning and sources of asymmetry. This model frequently ignores the fundamental assumption of ratios: the relationship between the variables entered in the ratio must be isometric. Violations of this assumption can obscure existing asymmetries and render spurious results. In this study, we examined the performance of the classic indices in detecting and portraying the asymmetry patterns in four human appendicular bones and explored potential methodological alternatives. Examination of the ratio model revealed that it does not fulfill its intended goals in the bones examined, as the numerator and denominator are independent in all cases. The ratios also introduced strong biases in the comparisons between different elements and variables, generating spurious asymmetry patterns. Multivariate analyses strongly suggest that any transformation to control for overall size or variable range must be conducted before, rather than after, calculating the asymmetries. A combination of exploratory multivariate techniques, such as Principal Components Analysis, and confirmatory linear methods, such as regression and analysis of covariance, appear as a promising and powerful alternative to the use of ratios. PMID:24842694

  10. Alternative Medicine and Your Child

    Science.gov (United States)

    ... to Know About Zika & Pregnancy Complementary and Alternative Medicine KidsHealth > For Parents > Complementary and Alternative Medicine Print ... works. previous continue How CAM Differs From Traditional Medicine CAM is frequently distinguished by its holistic methods, ...

  11. Pea Island Alternatives Development Report

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this Alternatives Development Report is to outline,in broad scope, the options, tools and alternatives available to the US Fish and Wildlife Service...

  12. Quantitative laser-induced breakdown spectroscopy data using peak area step-wise regression analysis: an alternative method for interpretation of Mars science laboratory results

    Energy Technology Data Exchange (ETDEWEB)

    Clegg, Samuel M [Los Alamos National Laboratory; Barefield, James E [Los Alamos National Laboratory; Wiens, Roger C [Los Alamos National Laboratory; Dyar, Melinda D [MT HOLYOKE COLLEGE; Schafer, Martha W [LSU; Tucker, Jonathan M [MT HOLYOKE COLLEGE

    2008-01-01

    The ChemCam instrument on the Mars Science Laboratory (MSL) will include a laser-induced breakdown spectrometer (LIBS) to quantify major and minor elemental compositions. The traditional analytical chemistry approach to calibration curves for these data regresses a single diagnostic peak area against concentration for each element. This approach contrasts with a new multivariate method in which elemental concentrations are predicted by step-wise multiple regression analysis based on areas of a specific set of diagnostic peaks for each element. The method is tested on LIBS data from igneous and metamorphosed rocks. Between 4 and 13 partial regression coefficients are needed to describe each elemental abundance accurately (i.e., with a regression line of R{sup 2} > 0.9995 for the relationship between predicted and measured elemental concentration) for all major and minor elements studied. Validation plots suggest that the method is limited at present by the small data set, and will work best for prediction of concentration when a wide variety of compositions and rock types has been analyzed.

  13. Comparative Evaluation of Phase 1 Results from the Energy Conversion Alternatives Study (ECAS). [coal utilization for electric power plants feasibility analysis

    Science.gov (United States)

    1976-01-01

    Ten advanced energy conversion systems for central-station, based-load electric power generation using coal and coal-derived fuels which were studied by NASA are presented. Various contractors were selected by competitive bidding to study these systems. A comparative evaluation is provided of the contractor results on both a system-by-system and an overall basis. Ground rules specified by NASA, such as coal specifications, fuel costs, labor costs, method of cost comparison, escalation and interest during construction, fixed charges, emission standards, and environmental conditions, are presented. Each system discussion includes the potential advantages of the system, the scope of each contractor's analysis, typical schematics of systems, comparison of cost of electricity and efficiency for each contractor, identification and reconciliation of differences, identification of future improvements, and discussion of outside comments. Considerations common to all systems, such as materials and furnaces, are also discussed. Results of selected in-house analyses are presented, in addition to contractor data. The results for all systems are then compared.

  14. SYNTHESIZABLE AND PROTOTYPIC VISUAL-TACTILE SYSTEM-IN FPGA: AN ALTERNATIVE TO ANALYSIS AND IMPROVEMENT OF THE VOICE QUALITY FOR THE HEARING IMPAIRED PEOPLE

    Directory of Open Access Journals (Sweden)

    Rodrigo Leone Alves

    2016-04-01

    Full Text Available Oral communication comprises one of the most important forms of social interaction. The process of learning the spoken language depends on the hearing, therefore, the total or partial loss of hearing sensitivity hinders such aspect. Digital signal processing techniques with non-invasive character are used for diagnosis, support and improvement of the voice quality of the deaf. Thus, the present study aims to propose and develop a system of analysis and correction of vocal disorders by means of visual and tactile feedback with module implemented in programmable device type FPGA (Field Programmable Gate Array. The results point to the potential of a proposed intervention as a helper for sensory substitution, being based on the monitoring and control of speech, in order to allow for the assessment and remediation by means of an electronic resource, allowing deaf individuals to obtain a support for learning the spoken language. The possibilities for improvements in communication skills observed in this study are dependent on the capability of the device together with the speech therapist, integrating therapies with the support of the family, the time and the motivation of the user, factors that cooperate for the success of this approach.

  15. Alternative splicing and muscular dystrophy

    OpenAIRE

    Pistoni, Mariaelena; Ghigna, Claudia; Gabellini, Davide

    2010-01-01

    Alternative splicing of pre-mRNAs is a major contributor to proteomic diversity and to the control of gene expression in higher eukaryotic cells. For this reasons, alternative splicing is tightly regulated in different tissues and developmental stages and its disruption can lead to a wide range of human disorders. The aim of this review is to focus on the relevance of alternative splicing for muscle function and muscle disease. We begin by giving a brief overview of alternative splicing, musc...

  16. Intuitions in Epistemology: Towards a Naturalistic Alternative

    DEFF Research Database (Denmark)

    Ahlström, Kristoffer

    2009-01-01

    The present paper revisits the main methodological problems with conceptual analysis and considers two attempts to rectify them in terms of prototypes and reflective equilibria, respectively. Finding both wanting for the purposes of epistemological analysis, a naturalistic alternative is then...... sketched that explores the positive implications of aforementioned problems for the demarcation of the respective roles of intuitions and empirical investigation within three epistemological domains, viz., the evaluation of epistemological hypotheses, the amelioration of epistemic practices, and the...

  17. Alternative Threat Methodology

    Directory of Open Access Journals (Sweden)

    Charles B. King III

    2011-01-01

    Full Text Available Of the many challenges facing risk analysis practitioners, perhaps the most difficult to overcome is in the field of terrorist threat analysis. When estimating the threat associated with naturally occurring events, historical data provides a great deal of insight into the frequency of those events. Threat associated with accidents applies many operations research tools to gauge future failure-rates (Failure Mode and Effects Analysis being perhaps the most widely known. However, estimating the probability of an individual's or group's attacking a specific (or even a generic target is an element of risk analysis in which art and intuition are applied far more regularly than is science.

  18. Significance analysis of the leachate level in a solid waste landfill in a coastal zone using total water balance and slope stability alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Koo, Ja-Kong; Do, Nam-Young [Korea Advanced Institute of Science & Technology, Taejon (Korea, Republic of)

    1996-12-31

    The K site near Seoul began landfilling in 1992. The landfilled wastes include municipal solid waste (66.4%), construction residues (20.4%), water and wastewater sludges (trace levels), and hazardous waste (trace levels). The water content of the municipal solid waste is very high (47.3%); as a result, the leachate level (average E.L.) of the landfill, the design value of which is 7.0 m, was measured at 10.3 m in January 1995 and is increasing. The increase of leachate level in the landfill site causes a problem with slope stability. The leachate level at each disposal stage divided by the intermediate cover layer was calculated with the HELP (Hydrologic Evaluation of Landfill Performance) model and calibrated with the data measured from February 1993 to June 1995. Also, the hydraulic conductivities of the waste layer and the intermediate cover layer in each stage were calibrated continuously with HELP model analysis. To verify these results, the total water balance in the landfill site was calculated using the infiltration rate calculated from HELP modeling. The leachate level was E.L. 10.0 m, which was close to the measured leachate level. To estimate the change of the leachate level in the future, the total water balances with different leachate discharge rates of 3,000, 3,500, and 5,000 m{sup 3}/day were analyzed. When the leachate discharge rate was 5,000 ton/day and the initial water content was decreased below 25%, the average leachate level was 10.8 m. This result satisfies the safety factor requirements (=1.3) for landfill slope stability. 4 refs., 8 figs., 1 tab.

  19. Functional analysis of splicing mutations in the IDS gene and the use of antisense oligonucleotides to exploit an alternative therapy for MPS II.

    Science.gov (United States)

    Matos, Liliana; Gonçalves, Vânia; Pinto, Eugénia; Laranjeira, Francisco; Prata, Maria João; Jordan, Peter; Desviat, Lourdes R; Pérez, Belén; Alves, Sandra

    2015-12-01

    Mucopolysaccharidosis II is a lysosomal storage disorder caused by mutations in the IDS gene, including exonic alterations associated with aberrant splicing. In the present work, cell-based splicing assays were performed to study the effects of two splicing mutations in exon 3 of IDS, i.e., c.241C>T and c.257C>T, whose presence activates a cryptic splice site in exon 3 and one in exon 8, i.e., c.1122C>T that despite being a synonymous mutation is responsible for the creation of a new splice site in exon 8 leading to a transcript shorter than usual. Mutant minigene analysis and overexpression assays revealed that SRSF2 and hnRNP E1 might be involved in the use and repression of the constitutive 3' splice site of exon 3 respectively. For the c.1122C>T the use of antisense therapy to correct the splicing defect was explored, but transfection of patient fibroblasts with antisense morpholino oligonucleotides (n=3) and a locked nucleic acid failed to abolish the abnormal transcript; indeed, it resulted in the appearance of yet another aberrant splicing product. Interestingly, the oligonucleotides transfection in control fibroblasts led to the appearance of the aberrant transcript observed in patients' cells after treatment, which shows that the oligonucleotides are masking an important cis-acting element for 5' splice site regulation of exon 8. These results highlight the importance of functional studies for understanding the pathogenic consequences of mis-splicing and highlight the difficulty in developing antisense therapies involving gene regions under complex splicing regulation. PMID:26407519

  20. Alternative tsunami models

    Energy Technology Data Exchange (ETDEWEB)

    Tan, A; Lyatskaya, I [Department of Physics, Alabama A and M University, Normal, AL 35762 (United States)], E-mail: arjun.tan@aamu.edu

    2009-01-15

    The interesting papers by Margaritondo (2005 Eur. J. Phys. 26 401) and by Helene and Yamashita (2006 Eur. J. Phys. 27 855) analysed the great Indian Ocean tsunami of 2004 using a simple one-dimensional canal wave model, which was appropriate for undergraduate students in physics and related fields of discipline. In this paper, two additional, easily understandable models, suitable for the same level of readership, are proposed: one, a two-dimensional model in flat space, and two, the same on a spherical surface. The models are used to study the tsunami produced by the central Kuril earthquake of November 2006. It is shown that the two alternative models, especially the latter one, give better representations of the wave amplitude, especially at far-flung locations. The latter model further demonstrates the enhancing effect on the amplitude due to the curvature of the Earth for far-reaching tsunami propagation.