WorldWideScience

Sample records for alternative reading frames

  1. ["Matreshka" Genes with Alternative Reading Frames].

    Science.gov (United States)

    Sheshukova, E V; Shindyapina, A V; Komarova, T V; Dorokhov, Yu L

    2016-02-01

    Although a relatively small part of the human genome contains protein encoding genes, the latest data on the discovery of alternative open reading frames (ORFs) in conventional mRNAs has highlighted the expanded coding potential of these genes. Until recently, it was believed that each mRNA transcript encodes a single protein. Recent proteogenomics data indicate the existence of exceptions to this rule, which greatly changes the usual meaning of the term "gene". The topology of a gene with overlapping ORFs resembles a Russian "matreshka" toy. There are two levels of "matreshka" genetic systems. First, the chromosomal level, when the "nested" gene is located within introns and exons of the main chromosomal gene, both in the sense and antisense orientation relative to the external gene. The second level is a mature mRNA molecule containing overlapping ORFs or an ORF with an alternative-start codon. In this review we will focus on the properties of "matreshka" genes of the second type and methods for their detection and verification. Particular attention is paid to the biological properties of the polypeptides encoded by these genes. PMID:27215029

  2. Norovirus regulation of the innate immune response and apoptosis occurs via the product of the alternative open reading frame 4.

    Directory of Open Access Journals (Sweden)

    Nora McFadden

    2011-12-01

    Full Text Available Small RNA viruses have evolved many mechanisms to increase the capacity of their short genomes. Here we describe the identification and characterization of a novel open reading frame (ORF4 encoded by the murine norovirus (MNV subgenomic RNA, in an alternative reading frame overlapping the VP1 coding region. ORF4 is translated during virus infection and the resultant protein localizes predominantly to the mitochondria. Using reverse genetics we demonstrated that expression of ORF4 is not required for virus replication in tissue culture but its loss results in a fitness cost since viruses lacking the ability to express ORF4 restore expression upon repeated passage in tissue culture. Functional analysis indicated that the protein produced from ORF4 antagonizes the innate immune response to infection by delaying the upregulation of a number of cellular genes activated by the innate pathway, including IFN-Beta. Apoptosis in the RAW264.7 macrophage cell line was also increased during virus infection in the absence of ORF4 expression. In vivo analysis of the WT and mutant virus lacking the ability to express ORF4 demonstrated an important role for ORF4 expression in infection and virulence. STAT1-/- mice infected with a virus lacking the ability to express ORF4 showed a delay in the onset of clinical signs when compared to mice infected with WT virus. Quantitative PCR and histopathological analysis of samples from these infected mice demonstrated that infection with a virus not expressing ORF4 results in a delayed infection in this system. In light of these findings we propose the name virulence factor 1, VF1 for this protein. The identification of VF1 represents the first characterization of an alternative open reading frame protein for the calicivirus family. The immune regulatory function of the MNV VF1 protein provide important perspectives for future research into norovirus biology and pathogenesis.

  3. Residues in the alternative reading frame tumor suppressor that influence its stability and p53-independent activities

    International Nuclear Information System (INIS)

    The Alternative Reading Frame (ARF) protein suppresses tumorigenesis through p53-dependent and p53-independent pathways. Most of ARF's anti-proliferative activity is conferred by sequences in its first exon. Previous work showed specific amino acid changes occurred in that region during primate evolution, so we programmed those changes into human p14ARF to assay their functional impact. Two human p14ARF residues (Ala14 and Thr31) were found to destabilize the protein while two others (Val24 and Ala41) promoted more efficient p53 stabilization and activation. Despite those effects, all modified p14ARF forms displayed robust p53-dependent anti-proliferative activity demonstrating there are no significant biological differences in p53-mediated growth suppression associated with simian versus human p14ARF residues. In contrast, p53-independent p14ARF function was considerably altered by several residue changes. Val24 was required for p53-independent growth suppression whereas multiple residues (Val24, Thr31, Ala41 and His60) enabled p14ARF to block or reverse the inherent chromosomal instability of p53-null MEFs. Together, these data pinpoint specific residues outside of established p14ARF functional domains that influence its expression and signaling activities. Most intriguingly, this work reveals a novel and direct role for p14ARF in the p53-independent maintenance of genomic stability.

  4. Hepatitis C Virus Frameshift/Alternate Reading Frame Protein Suppresses Interferon Responses Mediated by Pattern Recognition Receptor Retinoic-Acid-Inducible Gene-I

    Science.gov (United States)

    Park, Seung Bum; Seronello, Scott; Mayer, Wasima; Ojcius, David M.

    2016-01-01

    Hepatitis C virus (HCV) actively evades host interferon (IFN) responses but the mechanisms of how it does so are not completely understood. In this study, we present evidence for an HCV factor that contributes to the suppression of retinoic-acid-inducible gene-I (RIG-I)-mediated IFN induction. Expression of frameshift/alternate reading frame protein (F/ARFP) from HCV -2/+1 frame in Huh7 hepatoma cells suppressed type I IFN responses stimulated by HCV RNA pathogen-associated molecular pattern (PAMP) and poly(IC). The suppression occurred independently of other HCV factors; and activation of interferon stimulated genes, TNFα, IFN-λ1, and IFN-λ2/3 was likewise suppressed by HCV F/ARFP. Point mutations in the full-length HCV sequence (JFH1 genotype 2a strain) were made to introduce premature termination codons in the -2/+1 reading frame coding for F/ARFP while preserving the original reading frame, which enhanced IFNα and IFNβ induction by HCV. The potentiation of IFN response by the F/ARFP mutations was diminished in Huh7.5 cells, which already have a defective RIG-I, and by decreasing RIG-I expression in Huh7 cells. Furthermore, adding F/ARFP back via trans-complementation suppressed IFN induction in the F/ARFP mutant. The F/ARFP mutants, on the other hand, were not resistant to exogenous IFNα. Finally, HCV-infected human liver samples showed significant F/ARFP antibody reactivity, compared to HCV-uninfected control livers. Therefore, HCV F/ARFP likely cooperates with other viral factors to suppress type I and III IFN induction occurring through the RIG-I signaling pathway. This study identifies a novel mechanism of pattern recognition receptor modulation by HCV and suggests a biological function of the HCV alternate reading frame in the modulation of host innate immunity. PMID:27404108

  5. Fine mapping of the latency-related gene of herpes simplex virus type 1: alternative splicing produces distinct latency-related RNAs containing open reading frames

    International Nuclear Information System (INIS)

    The latency-related (LR) gene of herpes simplex virus type 1 (HSV-1) is transcriptionally active during HSV-1 latency, producing at least two LR-RNAs. The LR gene partially overlaps the immediate-early gene ICP0 and is transcribed in the opposite direction from ICP0, producing LR-RNAs that are complementary (antisense) to ICP0 mRNA. The LR gene is thought to be involved in HSV-1 latency. The authors report here the time mapping and partial sequence analysis of this HSV-1 LR gene. 32P-labeled genomic DNA restriction fragments and synthetic oligonucleotides were used as probes for in situ hybridizations and Northern (RNA) blot hybridizations of RNA from trigeminal ganglia of rabbits latently infected with HSV-1. The two most abundant LR-RNAs appeared to share their 5' and 3' ends and to be produced by alternative splicing. These LR-RNAs were approximately 2 and 1.3 to 1.5 kilobases in length and were designated LR-RNA 1 and LF-RNA 2, respectively. LR-RNA 1 appeared to have at least one intron removed, while LR-RNA 2 appeared to have at least two introns removed. The LR-RNAs contained two potential long open reading frames, suggesting the possibility that one or more of the LR-RNAs may be a functional mRNA

  6. Predicting statistical properties of open reading frames in bacterial genomes.

    Directory of Open Access Journals (Sweden)

    Katharina Mir

    Full Text Available An analytical model based on the statistical properties of Open Reading Frames (ORFs of eubacterial genomes such as codon composition and sequence length of all reading frames was developed. This new model predicts the average length, maximum length as well as the length distribution of the ORFs of 70 species with GC contents varying between 21% and 74%. Furthermore, the number of annotated genes is predicted with high accordance. However, the ORF length distribution in the five alternative reading frames shows interesting deviations from the predicted distribution. In particular, long ORFs appear more often than expected statistically. The unexpected depletion of stop codons in these alternative open reading frames cannot completely be explained by a biased codon usage in the +1 frame. While it is unknown if the stop codon depletion has a biological function, it could be due to a protein coding capacity of alternative ORFs exerting a selection pressure which prevents the fixation of stop codon mutations. The comparison of the analytical model with bacterial genomes, therefore, leads to a hypothesis suggesting novel gene candidates which can now be investigated in subsequent wet lab experiments.

  7. Selecting Open Reading Frames From DNA

    OpenAIRE

    Zacchi, Paola; Sblattero, Daniele; Florian, Fiorella; Marzari, Roberto; Bradbury, Andrew R. M.

    2003-01-01

    We describe a method to select DNA encoding functional open reading frames (ORFs) from noncoding DNA within the context of a specific vector. Phage display has been used as an example, but any system requiring DNA encoding protein fragments, for example, the yeast two-hybrid system, could be used. By cloning DNA fragments upstream of a fusion gene, consisting of the β-lactamase gene flanked by lox recombination sites, which is, in turn, upstream of gene 3 from fd phage, only those clones cont...

  8. West Nile alternative open reading frame (N-NS4B/WARF4 is produced in infected West Nile Virus (WNV cells and induces humoral response in WNV infected individuals

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    Faggioni Giovanni

    2012-11-01

    Full Text Available Abstract Background West Nile Virus (WNV is a flavivirus that requires an efficient humoral and cellular host response for the control of neuroinvasive infection. We previously reported the existence of six alternative open reading frame proteins in WNV genome, one of which entitled WARF4 is exclusively restricted to the lineage I of the virus. WARF4 is able to elicit antibodies in WNV infected horses; however, there was no direct experimental proof of the existence of this novel protein. The purpose of this study was to demonstrate the in vitro production of WARF4 protein following WNV infection of cultured VERO cells and its immunity in WNV infected individuals. Results We produced a monoclonal antibody against WARF4 protein (MAb 3A12 which detected the novel protein in WNV lineage I-infected, cultured VERO cells while it did not react with WNV lineage II infected cells. MAb 3A12 specificity to WARF4 protein was confirmed by its reactivity to only one peptide among four analyzed that cover the full WARF4 amino acids sequence. In addition, WARF4 protein was expressed in the late phase of WNV lineage I infection. Western blotting and bioinformatics analyses strongly suggest that the protein could be translated by programmed −1 ribosomal frameshifting process. Since WARF4 is embedded in the NS4B gene, we rename this novel protein N-NS4B/WARF4. Furthermore, serological analysis shows that N-NS4B/WARF4 is able to elicit antibodies in WNV infected individuals. Conclusions N-NS4B/WARF4 is the second Alternative Reading Frame (ARF protein that has been demonstrated to be produced following WNV infection and might represent a novel tool for a better characterization of immune response in WNV infected individuals. Further serological as well as functional studies are required to characterize the function of the N-NS4B/WARF4 protein. Since the virus might actually make an extensive use of ARFs, it appears important to investigate the novel six ARF

  9. New Characterizations of Riesz-Type Frames and Stability of Alternate Duals of Continuous Frames

    OpenAIRE

    Zhong-Qi Xiang

    2013-01-01

    We give new characterizations of Riesz-type frames, on equivalent conditions for a continuous frame to be a Riesz-type frame and on equivalency relations between Riesz-type frames and continuous frames. We characterize also the Riesz-type frames by using a bounded linear operator L. Finally, we study the stability of alternate duals of continuous frames and we prove that if two continuous frames are close to each other, then we can find alternate duals of them which are close to each other.

  10. Alternative approximation concepts for space frame synthesis

    Science.gov (United States)

    Lust, R. V.; Schmit, L. A.

    1985-01-01

    A structural synthesis methodology for the minimum mass design of 3-dimensionall frame-truss structures under multiple static loading conditions and subject to limits on displacements, rotations, stresses, local buckling, and element cross-sectional dimensions is presented. A variety of approximation concept options are employed to yield near optimum designs after no more than 10 structural analyses. Available options include: (A) formulation of the nonlinear mathematcal programming problem in either reciprocal section property (RSP) or cross-sectional dimension (CSD) space; (B) two alternative approximate problem structures in each design space; and (C) three distinct assumptions about element end-force variations. Fixed element, design element linking, and temporary constraint deletion features are also included. The solution of each approximate problem, in either its primal or dual form, is obtained using CONMIN, a feasible directions program. The frame-truss synthesis methodology is implemented in the COMPASS computer program and is used to solve a variety of problems. These problems were chosen so that, in addition to exercising the various approximation concepts options, the results could be compared with previously published work.

  11. Open Reading Frame Phylogenetic Analysis on the Cloud

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    Che-Lun Hung

    2013-01-01

    Full Text Available Phylogenetic analysis has become essential in researching the evolutionary relationships between viruses. These relationships are depicted on phylogenetic trees, in which viruses are grouped based on sequence similarity. Viral evolutionary relationships are identified from open reading frames rather than from complete sequences. Recently, cloud computing has become popular for developing internet-based bioinformatics tools. Biocloud is an efficient, scalable, and robust bioinformatics computing service. In this paper, we propose a cloud-based open reading frame phylogenetic analysis service. The proposed service integrates the Hadoop framework, virtualization technology, and phylogenetic analysis methods to provide a high-availability, large-scale bioservice. In a case study, we analyze the phylogenetic relationships among Norovirus. Evolutionary relationships are elucidated by aligning different open reading frame sequences. The proposed platform correctly identifies the evolutionary relationships between members of Norovirus.

  12. Open reading frame phylogenetic analysis on the cloud.

    Science.gov (United States)

    Hung, Che-Lun; Lin, Chun-Yuan

    2013-01-01

    Phylogenetic analysis has become essential in researching the evolutionary relationships between viruses. These relationships are depicted on phylogenetic trees, in which viruses are grouped based on sequence similarity. Viral evolutionary relationships are identified from open reading frames rather than from complete sequences. Recently, cloud computing has become popular for developing internet-based bioinformatics tools. Biocloud is an efficient, scalable, and robust bioinformatics computing service. In this paper, we propose a cloud-based open reading frame phylogenetic analysis service. The proposed service integrates the Hadoop framework, virtualization technology, and phylogenetic analysis methods to provide a high-availability, large-scale bioservice. In a case study, we analyze the phylogenetic relationships among Norovirus. Evolutionary relationships are elucidated by aligning different open reading frame sequences. The proposed platform correctly identifies the evolutionary relationships between members of Norovirus. PMID:23671843

  13. Reading between Texts: Thai Postgraduate Students' Intertextual Framing and Metacognition Use in Reading

    Science.gov (United States)

    Bell, Joyce

    2008-01-01

    Many factors influence students' reading practices. In the case of international students, home country educational practices and socio-cultural factors may pose a greater influence than for local students. This study reported here, part of a larger study, investigated the intertextual framing practices used in reading by a cohort of Thai…

  14. Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection

    OpenAIRE

    Au, Hilda H.; Cornilescu, Gabriel; Mouzakis, Kathryn D.; Ren, Qian; Burke, Jordan E.; Lee, Seonghoon; Butcher, Samuel E.; Jan, Eric

    2015-01-01

    Viruses use alternate mechanisms to increase the coding capacity of their viral genomes. The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts an RNA structure that can direct translation in 0 and +1 reading frames to produce the viral structural proteins and an overlapping ORFx product. Here we provide structural and biochemical evidence that the PKI domain of the IRES mimics a complete tRNA-like structure to facilitate reading frame selection and allows the viral IR...

  15. A Probabilistic Genome-Wide Gene Reading Frame Sequence Model

    DEFF Research Database (Denmark)

    Have, Christian Theil; Mørk, Søren

    We introduce a new type of probabilistic sequence model, that model the sequential composition of reading frames of genes in a genome. Our approach extends gene finders with a model of the sequential composition of genes at the genome-level -- effectively producing a sequential genome annotation as...... output. The model can be used to obtain the most probable genome annotation based on a combination of i: a gene finder score of each gene candidate and ii: the sequence of the reading frames of gene candidates through a genome. The model --- as well as a higher order variant --- is developed and tested...... using the probabilistic logic programming language and machine learning system PRISM - a fast and efficient model prototyping environment, using bacterial gene finding performance as a benchmark of signal strength. The model is used to prune a set of gene predictions from an underlying gene finder and...

  16. Upstream open reading frames: Molecular switches in (patho)physiology

    OpenAIRE

    Wethmar, Klaus; Smink, Jeske J.; Leutz, Achim

    2010-01-01

    Conserved upstream open reading frames (uORFs) are found within many eukaryotic transcripts and are known to regulate protein translation. Evidence from genetic and bioinformatic studies implicates disturbed uORF-mediated translational control in the etiology of human diseases. A genetic mouse model has recently provided proof-of-principle support for the physiological relevance of uORF-mediated translational control in mammals. The targeted disruption of the uORF initiation codon within the ...

  17. The canonical and alternate duals of a wavelet frame

    DEFF Research Database (Denmark)

    Lemvig, Jakob; Bownik, Marcin

    2007-01-01

    We show that there exists a frame wavelet ψ with fast decay in the time domain and compact support in the frequency domain generating a wavelet system whose canonical dual frame cannot be generated by an arbitrary number of generators. On the other hand, there exists infinitely many alternate duals...

  18. The canonical and alternate duals of a wavelet frame

    DEFF Research Database (Denmark)

    Bownik, Marcin; Lemvig, Jakob

    We show that there exists a frame wavelet $\\psi$ with fast decay in the time domain and compact support in the frequency domain generating a wavelet system whose canonical dual frame cannot be generated by an arbitrary number of generators. On the other hand, there exists infinitely many alternate...

  19. Oscillating evolution of a mammalian locus with overlapping reading frames: an XLalphas/ALEX relay.

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    Anton Nekrutenko

    2005-08-01

    Full Text Available XLalphas and ALEX are structurally unrelated mammalian proteins translated from alternative overlapping reading frames of a single transcript. Not only are they encoded by the same locus, but a specific XLalphas/ALEX interaction is essential for G-protein signaling in neuroendocrine cells. A disruption of this interaction leads to abnormal human phenotypes, including mental retardation and growth deficiency. The region of overlap between the two reading frames evolves at a remarkable speed: the divergence between human and mouse ALEX polypeptides makes them virtually unalignable. To trace the evolution of this puzzling locus, we sequenced it in apes, Old World monkeys, and a New World monkey. We show that the overlap between the two reading frames and the physical interaction between the two proteins force the locus to evolve in an unprecedented way. Namely, to maintain two overlapping protein-coding regions the locus is forced to have high GC content, which significantly elevates its intrinsic evolutionary rate. However, the two encoded proteins cannot afford to change too quickly relative to each other as this may impair their interaction and lead to severe physiological consequences. As a result XLalphas and ALEX evolve in an oscillating fashion constantly balancing the rates of amino acid replacements. This is the first example of a rapidly evolving locus encoding interacting proteins via overlapping reading frames, with a possible link to the origin of species-specific neurological differences.

  20. Oscillating Evolution of a Mammalian Locus with Overlapping Reading Frames: An XLalphas/ALEX Relay.

    Directory of Open Access Journals (Sweden)

    2005-08-01

    Full Text Available XLalphas and ALEX are structurally unrelated mammalian proteins translated from alternative overlapping reading frames of a single transcript. Not only are they encoded by the same locus, but a specific XLalphas/ALEX interaction is essential for G-protein signaling in neuroendocrine cells. A disruption of this interaction leads to abnormal human phenotypes, including mental retardation and growth deficiency. The region of overlap between the two reading frames evolves at a remarkable speed: the divergence between human and mouse ALEX polypeptides makes them virtually unalignable. To trace the evolution of this puzzling locus, we sequenced it in apes, Old World monkeys, and a New World monkey. We show that the overlap between the two reading frames and the physical interaction between the two proteins force the locus to evolve in an unprecedented way. Namely, to maintain two overlapping protein-coding regions the locus is forced to have high GC content, which significantly elevates its intrinsic evolutionary rate. However, the two encoded proteins cannot afford to change too quickly relative to each other as this may impair their interaction and lead to severe physiological consequences. As a result XLalphas and ALEX evolve in an oscillating fashion constantly balancing the rates of amino acid replacements. This is the first example of a rapidly evolving locus encoding interacting proteins via overlapping reading frames, with a possible link to the origin of species-specific neurological differences.

  1. Conserved upstream open reading frames in higher plants

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    Schultz Carolyn J

    2008-07-01

    Full Text Available Abstract Background Upstream open reading frames (uORFs can down-regulate the translation of the main open reading frame (mORF through two broad mechanisms: ribosomal stalling and reducing reinitiation efficiency. In distantly related plants, such as rice and Arabidopsis, it has been found that conserved uORFs are rare in these transcriptomes with approximately 100 loci. It is unclear how prevalent conserved uORFs are in closely related plants. Results We used a homology-based approach to identify conserved uORFs in five cereals (monocots that could potentially regulate translation. Our approach used a modified reciprocal best hit method to identify putative orthologous sequences that were then analysed by a comparative R-nomics program called uORFSCAN to find conserved uORFs. Conclusion This research identified new genes that may be controlled at the level of translation by conserved uORFs. We report that conserved uORFs are rare (

  2. Upstream open reading frames: Molecular switches in (patho)physiology

    Science.gov (United States)

    Wethmar, Klaus; Smink, Jeske J; Leutz, Achim

    2010-01-01

    Conserved upstream open reading frames (uORFs) are found within many eukaryotic transcripts and are known to regulate protein translation. Evidence from genetic and bioinformatic studies implicates disturbed uORF-mediated translational control in the etiology of human diseases. A genetic mouse model has recently provided proof-of-principle support for the physiological relevance of uORF-mediated translational control in mammals. The targeted disruption of the uORF initiation codon within the transcription factor CCAAT/enhancer binding protein β (C/EBPβ) gene resulted in deregulated C/EBPβ protein isoform expression, associated with defective liver regeneration and impaired osteoclast differentiation. The high prevalence of uORFs in the human transcriptome suggests that intensified search for mutations within 5′ RNA leader regions may reveal a multitude of alterations affecting uORFs, causing pathogenic deregulation of protein expression. PMID:20726009

  3. Detecting actively translated open reading frames in ribosome profiling data.

    Science.gov (United States)

    Calviello, Lorenzo; Mukherjee, Neelanjan; Wyler, Emanuel; Zauber, Henrik; Hirsekorn, Antje; Selbach, Matthias; Landthaler, Markus; Obermayer, Benedikt; Ohler, Uwe

    2016-02-01

    RNA-sequencing protocols can quantify gene expression regulation from transcription to protein synthesis. Ribosome profiling (Ribo-seq) maps the positions of translating ribosomes over the entire transcriptome. We have developed RiboTaper (available at https://ohlerlab.mdc-berlin.de/software/), a rigorous statistical approach that identifies translated regions on the basis of the characteristic three-nucleotide periodicity of Ribo-seq data. We used RiboTaper with deep Ribo-seq data from HEK293 cells to derive an extensive map of translation that covered open reading frame (ORF) annotations for more than 11,000 protein-coding genes. We also found distinct ribosomal signatures for several hundred upstream ORFs and ORFs in annotated noncoding genes (ncORFs). Mass spectrometry data confirmed that RiboTaper achieved excellent coverage of the cellular proteome. Although dozens of novel peptide products were validated in this manner, few of the currently annotated long noncoding RNAs appeared to encode stable polypeptides. RiboTaper is a powerful method for comprehensive de novo identification of actively used ORFs from Ribo-seq data. PMID:26657557

  4. Genome-Wide Search for Translated Upstream Open Reading Frames in Arabidopsis Thaliana.

    Science.gov (United States)

    Hu, Qiwen; Merchante, Catharina; Stepanova, Anna N; Alonso, Jose M; Heber, Steffen

    2016-03-01

    Upstream open reading frames (uORFs) are open reading frames that occur within the 5' UTR of an mRNA. uORFs have been found in many organisms. They play an important role in gene regulation, cell development, and in various metabolic processes. It is believed that translated uORFs reduce the translational efficiency of the main coding region. However, only few uORFs are experimentally characterized. In this paper, we use ribosome footprinting together with a semi-supervised approach based on stacking classification models to identify translated uORFs in Arabidopsis thaliana. Our approach identified 5360 potentially translated uORFs in 2051 genes. GO terms enriched in genes with translated uORFs include catalytic activity, binding, transferase activity, phosphotransferase activity, kinase activity, and transcription regulator activity. The reported uORFs occur with a higher frequency in multi-isoform genes, and some uORFs are affected by alternative transcript start sites or alternative splicing events. Association rule mining revealed sequence features associated with the translation status of the uORFs. We hypothesize that uORF translation is a complex process that might be regulated by multiple factors. The identified uORFs are available online at:https://www.dropbox.com/sh/zdutupedxafhly8/AABFsdNR5zDfiozB7B4igFcja?dl=0. This paper is the extended version of our research presented at ISBRA 2015. PMID:26886998

  5. Systematic Cloning of Treponema pallidum Open Reading Frames for Protein Expression and Antigen Discovery

    OpenAIRE

    McKevitt, Matthew; Patel, Krupa; Smajs, David; Marsh, Michael; McLoughlin, Melanie; Norris, Steven J; Weinstock, George M.; Palzkill, Timothy

    2003-01-01

    A topoisomerase-based method was used to clone PCR products encoding 991 of the 1041 open reading frames identified in the genome sequence of the bacterium that causes syphilis, Treponema pallidum subsp. pallidum. Cloning the open reading frames into the univector plasmid system permitted the rapid conversion of the original clone set to other functional vectors containing a variety of promoters or tag sequences. A computational prediction of signal sequences identifie...

  6. Anticodon loop mutations perturb reading frame maintenance by the E site tRNA

    OpenAIRE

    Sanders, Christina L.; Lohr, Kristin J.; Gambill, Holly L.; Curran, Ryan B.; Curran, James F.

    2008-01-01

    The ribosomal E site helps hold the reading frame. Certain tRNA mutations affect translation, and anticodon loop mutations can be especially detrimental. We studied the effects of mutations saturating the anticodon loop of the amber suppressor tRNA, Su7, on the ability to help hold the reading frame when in the E site. We also tested three mutations in the anticodon stem, as well as a mutation in the D stem (the “Hirsh” mutation). We used the Escherichia coli RF2 programmed frameshift site to...

  7. DNA vaccination of pigs with open reading frame 1-7 of PRRS virus

    DEFF Research Database (Denmark)

    Barfoed, Annette Malene; Blixenkrone-Møller, Merete; Jensen, Merethe Holm;

    2004-01-01

    We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with...

  8. DNA vaccination of pigs with open reading frame 1-7 of PRRS virus

    DEFF Research Database (Denmark)

    Barfoed, Annette Malene; Blixenkrone-Møller, Merete; Jensen, Merethe Holm; Bøtner, Anette; Kamstrup, Søren

    We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination with...

  9. Short-read reading-frame predictors are not created equal: sequence error causes loss of signal

    Directory of Open Access Journals (Sweden)

    Trimble William L

    2012-07-01

    Full Text Available Abstract Background Gene prediction algorithms (or gene callers are an essential tool for analyzing shotgun nucleic acid sequence data. Gene prediction is a ubiquitous step in sequence analysis pipelines; it reduces the volume of data by identifying the most likely reading frame for a fragment, permitting the out-of-frame translations to be ignored. In this study we evaluate five widely used ab initio gene-calling algorithms—FragGeneScan, MetaGeneAnnotator, MetaGeneMark, Orphelia, and Prodigal—for accuracy on short (75–1000 bp fragments containing sequence error from previously published artificial data and “real” metagenomic datasets. Results While gene prediction tools have similar accuracies predicting genes on error-free fragments, in the presence of sequencing errors considerable differences between tools become evident. For error-containing short reads, FragGeneScan finds more prokaryotic coding regions than does MetaGeneAnnotator, MetaGeneMark, Orphelia, or Prodigal. This improved detection of genes in error-containing fragments, however, comes at the cost of much lower (50% specificity and overprediction of genes in noncoding regions. Conclusions Ab initio gene callers offer a significant reduction in the computational burden of annotating individual nucleic acid reads and are used in many metagenomic annotation systems. For predicting reading frames on raw reads, we find the hidden Markov model approach in FragGeneScan is more sensitive than other gene prediction tools, while Prodigal, MGA, and MGM are better suited for higher-quality sequences such as assembled contigs.

  10. uPEPperoni: An online tool for upstream open reading frame location and analysis of transcript conservation

    OpenAIRE

    Skarshewski, Adam; Stanton-Cook, Mitchell; Huber, Thomas; Al Mansoori, Sumaya; Smith, Ross; Beatson, Scott A.; Rothnagel, Joseph A.

    2014-01-01

    Background Several small open reading frames located within the 5′ untranslated regions of mRNAs have recently been shown to be translated. In humans, about 50% of mRNAs contain at least one upstream open reading frame representing a large resource of coding potential. We propose that some upstream open reading frames encode peptides that are functional and contribute to proteome complexity in humans and other organisms. We use the term uPEPs to describe peptides encoded by upstream open read...

  11. A novel read-out IC allowing microbolometers to operate with high frame rate

    Science.gov (United States)

    Zhou, Yun; Lv, Jian; Wang, LuXia; Que, LongCheng; Jiang, YaDong

    2012-10-01

    This paper presents a new Read_out IC (ROIC) that uses two shared capacitances for integral and sampling. At similar power consumption and chip area, this ROIC architecture achieves a higher frame rate compared with the conventional architecture. A 384×288 uncooled microbolometer focal plane array (FPA) based on the proposed circuit was implemented on silicon using a 0.5 μm CMOS technology. Measurements show the proposed architecture enables the frame rate increase of 6.8% using the same master clock.

  12. Thousands of novel translated open reading frames in humans inferred by ribosome footprint profiling.

    Science.gov (United States)

    Raj, Anil; Wang, Sidney H; Shim, Heejung; Harpak, Arbel; Li, Yang I; Engelmann, Brett; Stephens, Matthew; Gilad, Yoav; Pritchard, Jonathan K

    2016-01-01

    Accurate annotation of protein coding regions is essential for understanding how genetic information is translated into function. We describe riboHMM, a new method that uses ribosome footprint data to accurately infer translated sequences. Applying riboHMM to human lymphoblastoid cell lines, we identified 7273 novel coding sequences, including 2442 translated upstream open reading frames. We observed an enrichment of footprints at inferred initiation sites after drug-induced arrest of translation initiation, validating many of the novel coding sequences. The novel proteins exhibit significant selective constraint in the inferred reading frames, suggesting that many are functional. Moreover, ~40% of bicistronic transcripts showed negative correlation in the translation levels of their two coding sequences, suggesting a potential regulatory role for these novel regions. Despite known limitations of mass spectrometry to detect protein expressed at low level, we estimated a 14% validation rate. Our work significantly expands the set of known coding regions in humans. PMID:27232982

  13. Comprehensive translational control of tyrosine kinase expression by upstream open reading frames

    OpenAIRE

    Wethmar, K; J. Schulz; Muro, E.M.; Talyan, S.; Andrade-Navarro, M A; Leutz, A

    2016-01-01

    Post-transcriptional control has emerged as a major regulatory event in gene expression and often occurs at the level of translation initiation. Although overexpression or constitutive activation of tyrosine kinases (TKs) through gene amplification, translocation or mutation are well-characterized oncogenic events, current knowledge about translational mechanisms of TK activation is scarce. Here, we report the presence of translational cis-regulatory upstream open reading frames (uORFs) in th...

  14. Varicella-Zoster Virus Open Reading Frame 48 Encodes an Active Nuclease

    OpenAIRE

    Mueller, Niklaus H.; Gilden, Don; Cohrs, Randall J.

    2013-01-01

    Based on a DNA sequence and relative genomic position similar to those other herpesviruses, varicella-zoster virus (VZV) open reading frame 48 (ORF48) is predicted to encode an alkaline nuclease. Here we report the cloning, expression, purification, and characterization of recombinant VZV ORF48 protein and a VZV ORF48 point mutation (T172P). Protein encoded by wild-type ORF48, but not mutant protein, displayed both endo- and exonuclease activity, identifying ORF48 as a potential therapeutic t...

  15. Visualising ribosome profiling and using it for reading frame detection and exploration of eukaryotic translation initiation

    OpenAIRE

    Mannion Michel, Audrey

    2013-01-01

    Ribosome profiling (ribo-seq) is a recently developed technique that provides genomewide information on protein synthesis (GWIPS) in vivo. The high resolution of ribo-seq is one of the exciting properties of this technique. In Chapter 2, I present a computational method that utilises the sub-codon precision and triplet periodicity of ribosome profiling data to detect transitions in the translated reading frame. Application of this method to ribosome profiling data generated for human HeLa cel...

  16. OrfM: a fast open reading frame predictor for metagenomic data

    Science.gov (United States)

    Woodcroft, Ben J.; Boyd, Joel A.

    2016-01-01

    Summary: Finding and translating stretches of DNA lacking stop codons is a task common in the analysis of sequence data. However, the computational tools for finding open reading frames are sufficiently slow that they are becoming a bottleneck as the volume of sequence data grows. This computational bottleneck is especially problematic in metagenomics when searching unassembled reads, or screening assembled contigs for genes of interest. Here, we present OrfM, a tool to rapidly identify open reading frames (ORFs) in sequence data by applying the Aho–Corasick algorithm to find regions uninterrupted by stop codons. Benchmarking revealed that OrfM finds identical ORFs to similar tools (‘GetOrf’ and ‘Translate’) but is four-five times faster. While OrfM is sequencing platform-agnostic, it is best suited to large, high quality datasets such as those produced by Illumina sequencers. Availability and Implementation: Source code and binaries are freely available for download at http://github.com/wwood/OrfM or through GNU Guix under the LGPL 3+ license. OrfM is implemented in C and supported on GNU/Linux and OSX. Contacts: b.woodcroft@uq.edu.au Supplementary information: Supplementary data are available at Bioinformatics online. PMID:27153669

  17. tRNA properties help shape codon pair preferences in open reading frames

    OpenAIRE

    Buchan, J. Ross; Aucott, Lorna S; Stansfield, Ian

    2006-01-01

    Translation elongation is an accurate and rapid process, dependent upon efficient juxtaposition of tRNAs in the ribosomal A- and P-sites. Here, we sought evidence of A- and P-site tRNA interaction by examining bias in codon pair choice within open reading frames from a range of genomes. Three distinct and marked effects were revealed once codon and dipeptide biases had been subtracted. First, in the majority of genomes, codon pair preference is primarily determined by a tetranucleotide combin...

  18. A read-out system for the Medipix2 chip capable of 500 frames per second

    International Nuclear Information System (INIS)

    High-speed X-ray-imaging acquisition technique is a growing field that can be used to understand microscopic mechanism of different phenomena in biology and material science. IFAE and CNM developed a very high-speed readout system, named DEMAS, for the Medipix2. The system is able to read a single Medipix2 chip through the parallel bus at a rate of 1 kHz.With a duty cycle of 50%, the real sampling speed is 500 frames per second (fps). This implies that 1 ms is allocated to the exposure time and another millisecond is devoted to the read-out of the chip. In such configuration, the raw data throughput is about 500 Mbit/s. For the first time we present examples of acquisition at 500 fps of moving samples with X-rays working in direct capture and photon counting mode

  19. The upstream open reading frame of cyclin-dependent kinase inhibitor 1A mRNA negatively regulates translation of the downstream main open reading frame

    International Nuclear Information System (INIS)

    Highlights: ► CDKN1A mRNA is a bona fide NMD substrate. ► The uORF of CDKN1A mRNA is efficiently translated. ► Translation of downstream main ORF is negatively regulated by translation of uORF in CDKN1A mRNA. -- Abstract: The first round of translation occurs on mRNAs bound by nuclear cap-binding complex (CBC), which is composed of nuclear cap-binding protein 80 and 20 (CBP80/20). During this round of translation, aberrant mRNAs are recognized and downregulated in abundance by nonsense-mediated mRNA decay (NMD), which is one of the mRNA quality control mechanisms. Here, our microarray analysis reveals that the level of cyclin-dependent kinase inhibitor 1A (CDKN1A; also known as Waf1/p21) mRNAs increases in cells depleted of cellular NMD factors. Intriguingly, CDKN1A mRNA contains an upstream open reading frame (uORF), which is a NMD-inducing feature. Using chimeric reporter constructs, we find that the uORF of CDKN1A mRNA negatively modulates translation of the main downstream ORF. These findings provide biological insights into the possible role of NMD in diverse biological pathways mediated by CDKN1A.

  20. An upstream open reading frame is essential for feedback regulation of ascorbate biosynthesis in Arabidopsis.

    Science.gov (United States)

    Laing, William A; Martínez-Sánchez, Marcela; Wright, Michele A; Bulley, Sean M; Brewster, Di; Dare, Andrew P; Rassam, Maysoon; Wang, Daisy; Storey, Roy; Macknight, Richard C; Hellens, Roger P

    2015-03-01

    Ascorbate (vitamin C) is an essential antioxidant and enzyme cofactor in both plants and animals. Ascorbate concentration is tightly regulated in plants, partly to respond to stress. Here, we demonstrate that ascorbate concentrations are determined via the posttranscriptional repression of GDP-l-galactose phosphorylase (GGP), a major control enzyme in the ascorbate biosynthesis pathway. This regulation requires a cis-acting upstream open reading frame (uORF) that represses the translation of the downstream GGP open reading frame under high ascorbate concentration. Disruption of this uORF stops the ascorbate feedback regulation of translation and results in increased ascorbate concentrations in leaves. The uORF is predicted to initiate at a noncanonical codon (ACG rather than AUG) and encode a 60- to 65-residue peptide. Analysis of ribosome protection data from Arabidopsis thaliana showed colocation of high levels of ribosomes with both the uORF and the main coding sequence of GGP. Together, our data indicate that the noncanonical uORF is translated and encodes a peptide that functions in the ascorbate inhibition of translation. This posttranslational regulation of ascorbate is likely an ancient mechanism of control as the uORF is conserved in GGP genes from mosses to angiosperms. PMID:25724639

  1. The role of upstream sequences in selecting the reading frame on tmRNA

    Directory of Open Access Journals (Sweden)

    Dewey Jonathan D

    2008-06-01

    Full Text Available Abstract Background tmRNA acts first as a tRNA and then as an mRNA to rescue stalled ribosomes in eubacteria. Two unanswered questions about tmRNA function remain: how does tmRNA, lacking an anticodon, bypass the decoding machinery and enter the ribosome? Secondly, how does the ribosome choose the proper codon to resume translation on tmRNA? According to the -1 triplet hypothesis, the answer to both questions lies in the unique properties of the three nucleotides upstream of the first tmRNA codon. These nucleotides assume an A-form conformation that mimics the codon-anticodon interaction, leading to recognition by the decoding center and choice of the reading frame. The -1 triplet hypothesis is important because it is the most credible model in which direct binding and recognition by the ribosome sets the reading frame on tmRNA. Results Conformational analysis predicts that 18 triplets cannot form the correct structure to function as the -1 triplet of tmRNA. We tested the tmRNA activity of all possible -1 triplet mutants using a genetic assay in Escherichia coli. While many mutants displayed reduced activity, our findings do not match the predictions of this model. Additional mutagenesis identified sequences further upstream that are required for tmRNA function. An immunoblot assay for translation of the tmRNA tag revealed that certain mutations in U85, A86, and the -1 triplet sequence result in improper selection of the first codon and translation in the wrong frame (-1 or +1 in vivo. Conclusion Our findings disprove the -1 triplet hypothesis. The -1 triplet is not required for accommodation of tmRNA into the ribosome, although it plays a minor role in frame selection. Our results strongly disfavor direct ribosomal recognition of the upstream sequence, instead supporting a model in which the binding of a separate ligand to A86 is primarily responsible for frame selection.

  2. Tying Down Loose Ends in the Chlamydomonas Genome: Functional Significance of Abundant Upstream Open Reading Frames

    Directory of Open Access Journals (Sweden)

    Frederick R. Cross

    2016-02-01

    Full Text Available The Chlamydomonas genome has been sequenced, assembled, and annotated to produce a rich resource for genetics and molecular biology in this well-studied model organism. The annotated genome is very rich in open reading frames upstream of the annotated coding sequence (‘uORFs’: almost three quarters of the assigned transcripts have at least one uORF, and frequently more than one. This is problematic with respect to the standard ‘scanning’ model for eukaryotic translation initiation. These uORFs can be grouped into three classes: class 1, initiating in-frame with the coding sequence (CDS (thus providing a potential in-frame N-terminal extension; class 2, initiating in the 5′ untranslated sequences (5UT and terminating out-of-frame in the CDS; and class 3, initiating and terminating within the 5UT. Multiple bioinformatics criteria (including analysis of Kozak consensus sequence agreement and BLASTP comparisons to the closely related Volvox genome, and statistical comparison to cds and to random sequence controls indicate that of ∼4000 class 1 uORFs, approximately half are likely in vivo translation initiation sites. The proposed resulting N-terminal extensions in many cases will sharply alter the predicted biochemical properties of the encoded proteins. These results suggest significant modifications in ∼2000 of the ∼20,000 transcript models with respect to translation initiation and encoded peptides. In contrast, class 2 uORFs may be subject to purifying selection, and the existent ones (surviving selection are likely inefficiently translated. Class 3 uORFs are found in more than half of transcripts, frequently multiple times per transcript; however, they are remarkably similar to random sequence expectations with respect to size, number, and composition, and therefore may in most cases be selectively neutral.

  3. SEQUENCE CONSERVATION OF HUMAN CYTOMEGALOVIRUS UL140 OPEN READING FRAME IN CLINICAL STRAINS

    Institute of Scientific and Technical Information of China (English)

    Ying Qi; Yan-ping Ma; Rong He; Zheng-rong Sun; Yaohua Ji; Yu-jing Huang; Qiang Ruan

    2008-01-01

    Objective To investigate the variability of human cytomegalovirus (HCMV) UL140 open reading frame (ORF)in clinical strains, and to explore the relationship between the variability of UL140 ORF and different symptoms of HC-MV infection.Methods HCMV ULI40 ORF was amplified by polymerase chain reaction and sequenced selectedly in 30 clinical strains.Results UL140 ORF of all clinical strains was amplified successfully. Compared with that of Toledo strain, the nncleotide and amino acid sequence identifies among all strains were 96.5% -100.0% and 95.2% -100.0%, respective-ly. All of the nucleotide changes were substitutions. The post-translational modification sites were conserved. The result of phylogenetic tree showed that the strains did not cluster according to different clinical symptoms.Conclusion HCMV UL140 ORF in clinical strains is highly conserved, which may play an important role in HC-MV infection.

  4. Minimum probe length for unique identification of all open reading frames in a microbial genome

    Energy Technology Data Exchange (ETDEWEB)

    Sokhansanj, B A; Ng, J; Fitch, J P

    2000-03-05

    In this paper, we determine the minimum hybridization probe length to uniquely identify at least 95% of the open reading frame (ORF) in an organism. We analyze the whole genome sequences of 17 species, 11 bacteria, 4 archaea, and 2 eukaryotes. We also present a mathematical model for minimum probe length based on assuming that all ORFs are random, of constant length, and contain an equal distribution of bases. The model accurately predicts the minimum probe length for all species, but it incorrectly predicts that all ORFs may be uniquely identified. However, a probe length of just 9 bases is adequate to identify over 95% of the ORFs for all 15 prokaryotic species we studied. Using a minimum probe length, while accepting that some ORFs may not be identified and that data will be lost due to hybridization error, may result in significant savings in microarray and oligonucleotide probe design.

  5. Long Open Reading Frame Transcripts Escape Nonsense-Mediated mRNA Decay in Yeast

    Directory of Open Access Journals (Sweden)

    Laurence Decourty

    2014-02-01

    Full Text Available Nonsense-mediated mRNA decay (NMD destabilizes eukaryotic transcripts with long 3′ UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and open reading frame (ORF regions and with the same long, destabilizing 3′ UTR. We developed a barcode-based DNA microarray strategy to compare the levels of each reporter mRNA in strains with or without active NMD. The size of the coding region had a significant negative effect on NMD efficiency. This effect was not specific to the tested 3′ UTR because two other different NMD reporters became less sensitive to NMD when ORF length was increased. Inefficient NMD was not due to a lack of association of Upf1 to long ORF transcripts. In conclusion, in addition to a long 3′ UTR, short translation length is an important feature of NMD substrates in yeast.

  6. Long open reading frame transcripts escape nonsense-mediated mRNA decay in yeast.

    Science.gov (United States)

    Decourty, Laurence; Doyen, Antonia; Malabat, Christophe; Frachon, Emmanuel; Rispal, Delphine; Séraphin, Bertrand; Feuerbach, Frank; Jacquier, Alain; Saveanu, Cosmin

    2014-02-27

    Nonsense-mediated mRNA decay (NMD) destabilizes eukaryotic transcripts with long 3' UTRs. To investigate whether other transcript features affect NMD, we generated yeast strains expressing chromosomal-derived mRNAs with 979 different promoter and open reading frame (ORF) regions and with the same long, destabilizing 3' UTR. We developed a barcode-based DNA microarray strategy to compare the levels of each reporter mRNA in strains with or without active NMD. The size of the coding region had a significant negative effect on NMD efficiency. This effect was not specific to the tested 3' UTR because two other different NMD reporters became less sensitive to NMD when ORF length was increased. Inefficient NMD was not due to a lack of association of Upf1 to long ORF transcripts. In conclusion, in addition to a long 3' UTR, short translation length is an important feature of NMD substrates in yeast. PMID:24529707

  7. An Alternative to Mapping a Word onto a Concept in Language Acquisition: Pragmatic Frames

    Science.gov (United States)

    Rohlfing, Katharina J.; Wrede, Britta; Vollmer, Anna-Lisa; Oudeyer, Pierre-Yves

    2016-01-01

    The classic mapping metaphor posits that children learn a word by mapping it onto a concept of an object or event. However, we believe that a mapping metaphor cannot account for word learning, because even though children focus attention on objects, they do not necessarily remember the connection between the word and the referent unless it is framed pragmatically, that is, within a task. Our theoretical paper proposes an alternative mechanism for word learning. Our main premise is that word learning occurs as children accomplish a goal in cooperation with a partner. We follow Bruner’s (1983) idea and further specify pragmatic frames as the learning units that drive language acquisition and cognitive development. These units consist of a sequence of actions and verbal behaviors that are co-constructed with a partner to achieve a joint goal. We elaborate on this alternative, offer some initial parametrizations of the concept, and embed it in current language learning approaches. PMID:27148105

  8. An Alternative to Mapping a Word onto a Concept in Language Acquisition: Pragmatic Frames.

    Science.gov (United States)

    Rohlfing, Katharina J; Wrede, Britta; Vollmer, Anna-Lisa; Oudeyer, Pierre-Yves

    2016-01-01

    The classic mapping metaphor posits that children learn a word by mapping it onto a concept of an object or event. However, we believe that a mapping metaphor cannot account for word learning, because even though children focus attention on objects, they do not necessarily remember the connection between the word and the referent unless it is framed pragmatically, that is, within a task. Our theoretical paper proposes an alternative mechanism for word learning. Our main premise is that word learning occurs as children accomplish a goal in cooperation with a partner. We follow Bruner's (1983) idea and further specify pragmatic frames as the learning units that drive language acquisition and cognitive development. These units consist of a sequence of actions and verbal behaviors that are co-constructed with a partner to achieve a joint goal. We elaborate on this alternative, offer some initial parametrizations of the concept, and embed it in current language learning approaches. PMID:27148105

  9. HIGH VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL150 OPEN READING FRAME IN LOW-PASSAGED CLINICAL ISOLATES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective To investigate the polymorphism of human cytomegalovirus (HCMV) UL150 open reading frame(ORF) in low-passaged clinical isolates, and to study the relationship between the polymorphism and different pathogenesis of congenital HCMV infection.Methods PCR was performed to amplify the entire HCMV UL150 ORF region of 29 clinical isolates, which hadbeen proven containing detectable HCMV-DNA using fluorescence quantitative PCR. PCR amplifcation products weresequenced directly, and the data were analyzed.Results Totally 25 among 29 isolates were amplified, and 18 isolates were sequenced successfully. HCMVUL150 ORF sequences derived from congenitally infected infants were high variability. The UL150 ORF in all 18 clinical isolates shifted backward by 8 nucleotides leading to frame-shift, and contained a single nucleotide deletion at nucleotide position 226 compared with that of Toledo strain. The nucleotide diversity was 0. 1% to 6. 8% and the amino acid diversity was 0. 2% to 19. 2% related to Toledo strain. However, the nucleotide diversity was 0. 1% to 6.4% and amino acid diversity was 0. 2% to 8.3% by compared with Merlin strain. Compared with Toledo, 4 new cysteine residues and 13 additional posttranslational modification sites were observed in UL150 putative proteins of clinical isolates. Moreover, the UL150 putative protein contained an additional transmembrane helix at position of 4-17 amino acid related to Toledo.Conclusion HCMV UL150 ORF and deduced amino acid sequences of clinical strains are hypervariability. No obvious linkage between the polymorphism and different pathogenesis of congenital HCMV infection is found.

  10. Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein.

    Science.gov (United States)

    Nawagitgul, P; Morozov, I; Bolin, S R; Harms, P A; Sorden, S D; Paul, P S

    2000-09-01

    Porcine circovirus 2 (PCV2), a single-stranded DNA virus associated with post-weaning multisystemic wasting syndrome of swine, has two potential open reading frames, ORF1 and ORF2, greater than 600 nucleotides in length. ORF1 is predicted to encode a replication-associated protein (Rep) essential for replication of viral DNA, while ORF2 contains a conserved basic amino acid sequence at the N terminus resembling that of the major structural protein of chicken anaemia virus. Thus far, the structural protein(s) of PCV2 have not been identified. In this study, a viral structural protein of 30 kDa was identified in purified PCV2 particles. ORF2 of PCV2 was cloned into a baculovirus expression vector and the gene product was expressed in insect cells. The expressed ORF2 gene product had a molecular mass of 30 kDa, similar to that detected in purified virus particles. The recombinant ORF2 protein self-assembled to form capsid-like particles when viewed by electron microscopy. Antibodies against the ORF2 protein were detected in samples of sera obtained from pigs as early as 3 weeks after experimental infection with PCV2. These results show that the major structural protein of PCV2 is encoded by ORF2 and has a molecular mass of 30 kDa. PMID:10950986

  11. Translational control of protein kinase Ceta by two upstream open reading frames.

    Science.gov (United States)

    Raveh-Amit, Hadas; Maissel, Adva; Poller, Jonathan; Marom, Liraz; Elroy-Stein, Orna; Shapira, Michal; Livneh, Etta

    2009-11-01

    Protein kinase C (PKC) represents a family of serine/threonine kinases that play a central role in the regulation of cell growth, differentiation, and transformation. Posttranslational control of the PKC isoforms and their activation have been extensively studied; however, not much is known about their translational regulation. Here we report that the expression of one of the PKC isoforms, PKCeta, is regulated at the translational level both under normal growth conditions and during stress imposed by amino acid starvation, the latter causing a marked increase in its protein levels. The 5' untranslated region (5' UTR) of PKCeta is unusually long and GC rich, characteristic of many oncogenes and growth regulatory genes. We have identified two conserved upstream open reading frames (uORFs) in its 5' UTR and show their effect in suppressing the expression of PKCeta in MCF-7 growing cells. While the two uORFs function as repressive elements that maintain low basal levels of PKCeta in growing cells, they are required for its enhanced expression upon amino acid starvation. We show that the translational regulation during stress involves leaky scanning and is dependent on eIF-2alpha phosphorylation by GCN2. Our work further suggests that translational regulation could provide an additional level for controlling the expression of PKC family members, being more common than currently recognized. PMID:19797084

  12. SEQUENCE VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL144 OPEN READING FRAME IN LOW-PASSAGE CLINICAL ISOLATES

    Institute of Scientific and Technical Information of China (English)

    Rong He; Yao-hua Ji; Qiang Ruan; Chang Xia; Lan-qing Liu; Sheng-min Lü; Ying Lu; Ying Qi; Yan-ping Ma; Qing Liu

    2004-01-01

    Objective To explore the relationship between human cytomegalovirus (HCMV) UL144 sequence variability and clinical disease.Methods HCMV UL144 open reading frame (ORF) was amplified by PCR assay in 72 lowpassage isolates [65 congenitally infective children and 7 healthy children who were HCMV-DNA positive by quantitative PCR (qPCR)]. All positive PCR products were analyzed by heteroduplex mobility assay and single-stranded conformation polymorphism (HMA-SSCP) and 32 of them were sequenced.Resuits Fifty-five patient isolates and five healthy children isolates were HCMV-UL144 positive by PCR. Sequencing and HMA-SSCP analysis showed that significant strain-specific variability was present in the UL144 ORF. Phylogenetic analysis indicated that the nucleotide sequences could be separated into 3 major genotypes. Comparing between UL144 sequences and the corresponding symptoms showed that genotype 2 did not exist in megacolon isolates. And genotype 1 and 3 were the major types among microcephaly and jaundice isolates respectively.Conclusions HCMV-UL144 existed in most of low passage isolates and sequences were hypervariable. The UL144ORF and its predicted product with the high level of sequence variability in different kinds of isolates suggest that UL144ORF might play a role in HCMV infectivity and subsequent diseases.

  13. Genetic characterization of complete open reading frame of glycoprotein C gene of bovine herpesvirus 1

    Directory of Open Access Journals (Sweden)

    Saurabh Majumder

    2013-10-01

    Full Text Available Aim: To characterize one of the major glycoprotein genes viz., glycoprotein C (gC; UL44, unique long region 44 of bovineherpesvirus 1(BoHV1 of Indian origin at genetic and phylogenetic level.Materials and Methods: A bovine herpesvirus 1 isolate viz., (BoHV1/IBR 216 II/ 1976/ India maintained at Division ofVirology, IVRI, Mukteswar was used for the current study. The DNA was extracted using commercial kit and the completeORF of gC gene was amplified, cloned, and sequenced by conventional Sanger sequencing method. The sequence wasgenetically and phylogenetically analysed using various bioinformatic tools. The sequence was submitted in the Genbankwith accession number Kc756965.Results: The complete ORF of gC gene was amplified and sequenced. It showed 100% sequence homology with referencecooper strain of BoHV1 and divergence varied from 0% to 2.7% with other isolates of BoHV1. The isolate under study haddivergence of 9.2%, 13%, 26.6%, and 9.2% with BoHV5 (Bovine herpesvirus 5, CvHV1 (Cervid herpesvirus 1, CpHV1(Caprine herpesvirus 1, and BuHV1 (Bubaline herpesvirus 1, respectively.Conclusion: This is the first genetic characterization of complete open reading frame (ORF of glycoprotein C gene (UL44 ofIndian isolate of BoHV1. The gC gene of BoHV1 is highly conserved among all BoHV1 isolates and it can be used as a targetfor designing diagnostic primers for the specific detection of BoHV1.

  14. Comprehensive translational control of tyrosine kinase expression by upstream open reading frames.

    Science.gov (United States)

    Wethmar, K; Schulz, J; Muro, E M; Talyan, S; Andrade-Navarro, M A; Leutz, A

    2016-03-31

    Post-transcriptional control has emerged as a major regulatory event in gene expression and often occurs at the level of translation initiation. Although overexpression or constitutive activation of tyrosine kinases (TKs) through gene amplification, translocation or mutation are well-characterized oncogenic events, current knowledge about translational mechanisms of TK activation is scarce. Here, we report the presence of translational cis-regulatory upstream open reading frames (uORFs) in the majority of transcript leader sequences of human TK mRNAs. Genetic ablation of uORF initiation codons in TK transcripts resulted in enhanced translation of the associated downstream main protein-coding sequences (CDSs) in all cases studied. Similarly, experimental removal of uORF start codons in additional non-TK proto-oncogenes, and naturally occurring loss-of-uORF alleles of the c-met proto-oncogene (MET) and the kinase insert domain receptor (KDR), was associated with increased CDS translation. Based on genome-wide sequence analyses we identified polymorphisms in 15.9% of all human genes affecting uORF initiation codons, associated Kozak consensus sequences or uORF-related termination codons. Together, these data suggest a comprehensive role of uORF-mediated translational control and delineate how aberrant induction of proto-oncogenes through loss-of-function mutations at uORF initiation codons may be involved in the etiology of cancer. We provide a detailed map of uORFs across the human genome to stimulate future research on the pathogenic role of uORFs. PMID:26096937

  15. Genomic Interspecies Microarray Hybridization: Rapid Discovery of Three Thousand Genes in the Maize Endophyte, Klebsiella pneumoniae 342, by Microarray Hybridization with Escherichia coli K-12 Open Reading Frames

    OpenAIRE

    Dong, Yuemei; Glasner, Jeremy D.; Blattner, Frederick R.; Triplett, Eric W.

    2001-01-01

    In an effort to efficiently discover genes in the diazotrophic endophyte of maize, Klebsiella pneumoniae 342, DNA from strain 342 was hybridized to a microarray containing 96% (n = 4,098) of the annotated open reading frames from Escherichia coli K-12. Using a criterion of 55% identity or greater, 3,000 (70%) of the E. coli K-12 open reading frames were also found to be present in strain 342. Approximately 24% (n = 1,030) of the E. coli K-12 open reading frames are absent in strain 342. For 1...

  16. Immunological and biochemical characterisation of 7ap, a short protein translated from an alternative frame of ORF7 of PRRSV.

    Science.gov (United States)

    Olasz, Ferenc; Dénes, Béla; Bálint, Ádám; Magyar, Tibor; Belák, Sándor; Zádori, Zoltán

    2016-06-01

    Sequence analysis revealed a short alternative open reading frame (ORF) named ORF7a within the nucleocapsid gene of genetically divergent porcine reproductive and respiratory syndrome virus (PRRSV) genomes. Alignment of the corresponding protein sequences (named 7ap) revealed substantial heterogeneity among 7aps of different genotypes, though all of them are predicted to be positively charged. Green fluorescent protein and FLAG fusion constructs of ORF7a of the HU-14432/2011 PRRSV demonstrated that 7ap is expressed. 7ap of HU- 14432/2011 (Hu7ap) was synthesised chemically, and ELISA experiments revealed that Hu7ap binds strongly to mammalian IgGs. Protein-protein gel retardation assays and complement fixation inhibition suggest that 7aps bind to the CH2 domain of the IgG(Fc) fragment. Cellular localisation and immunological characteristics of PRRSV 7ap may indicate multiple functions including nuclear and cytoplasmic over-tuning of normal cellular processes and immunosuppression. PMID:27342098

  17. Research on a Frame-Based Model of Reading Comprehension. Final Report.

    Science.gov (United States)

    Goldstein, Ira

    This report summarizes computational investigations of language comprehension based on Marvin Minsky's theory of frames, a recent advance in artifical intelligence theories about the representation of knowledge. The investigations discussed explored frame theory as a basis for text comprehension by implementing models of the theory and developing…

  18. Creating Joint Attentional Frames and Pointing to Evidence in the Reading and Writing Process

    Science.gov (United States)

    Unger, John A.; Liu, Rong; Scullion, Vicki A.

    2015-01-01

    This theory-into-practice paper integrates Tomasello's concept of Joint Attentional Frames and well-known ideas related to the work of Russian psychologist, Lev Vygotsky, with more recent ideas from social semiotics. Classroom procedures for incorporating student-created Joint Attentional Frames into literacy lessons are explained by links to…

  19. Facilitating Reading Acquisition in Multilingual Environments in India (FRAME-India). Final Report

    Science.gov (United States)

    Nakamura, Pooja; de Hoop, Thomas

    2014-01-01

    Most of the world is multilingual--multilingual at the national level (policies), at the community and family level (practices), and at the individual level (cognitive)--and each of these has implications for teaching and learning. Yet, at present, most reading decisions are not based on empirical research of how children learn to read in…

  20. On characterization and stability of alternate dual of g-frames

    OpenAIRE

    Arefijamaal, Ali Akbar; GHASEMI, SOHEILA

    2013-01-01

    One of the essential applications of frames is that they lead to expansions of vectors in the underlying Hilbert space in terms of the frame elements. In this decomposition, dual frames have a key role. G-frames, introduced by Sun, cover many other recent generalizations of frames. In this paper, we give some characterizations of dual g-frames. Moreover, we prove that if two g-frames are close to each other, then we can find duals of them which are close to each other.

  1. A Well-Ordered Society as a Democratic Community: Alternative Readings of Rawls’ Political Theory

    OpenAIRE

    Matan, Ana

    2005-01-01

    The standard reading of Rawls’ political theory puts great emphasis on Rawls’ liberalism and the primacy of basic liberties. The standard reading usually ignores Rawls’ remark that he is trying to develop most appropriate moral basis for a democratic society. An alternative reading, offered in the article, tries to bring out the democratic elements in Rawls’ thought and present Rawls’ concern for social justice as an attempt to offer a plausible account of a democratic communit...

  2. Kaposi's sarcoma-associated herpesvirus vCyclin open reading frame contains an internal ribosome entry site

    OpenAIRE

    Bieleski, L; Talbot, Simon

    2001-01-01

    We have previously examined the transcription and splicing of open reading frames (ORFS) 71 (K13), 72, and 73 of Kaposi's sarcoma-associated herpesvirus (KSHV) in the primary effusion lymphoma cell line BCP-1 (latently infected with KSHV) (45). The three genes encoded by these ORFs (for vFLIP, vCyclin, and latency-associated nuclear antigen [LANA]) are transcribed from a common transcription start site in BCP-1 cells during both latency and the lytic cycles. The resulting transcript is splice...

  3. Online Prelectures: An Alternative to Textbook Reading Assignments

    Science.gov (United States)

    Sadaghiani, Homeyra R.

    2012-05-01

    To engage students in a more meaningful discussion of course material and prompt their higher thinking skills, most instructors expect students to read the course textbook for initial exposure to the course content before class. However, as many instructors are aware, most students do not read their textbook throughout the quarter.1,2 At California State Polytechnic University, Pomona (Cal Poly Pomona) we have adopted web-based multimedia learning modules (MLMs) as prelecture assignments to help students to prepare for the class activities. The MLMs place lecture contents into the hands and control of the learners; similar to "flipped"3 or "inverted"4 classroom approaches, this method allows students to receive key course content outside of class and apply and analyze the content actively during class. In addition to initial exposure to basic principle, the MLMs provide additional worked examples that cannot be thoroughly covered in class.

  4. Identification and characterization of Kaposi's sarcoma-associated herpesvirus open reading frame 11 promotor activation

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Lei [Los Alamos National Laboratory

    2008-01-01

    Open reading frame 11 (ORF11) of Kaposi's sarcoma-associated herpesvirus belongs to a herpesviral homologous protein family shared by some members of the gamma- herpesvirus subfamily. Little is known about this ORF11 homologous protein family. We have characterized an unknown open reading frame, ORF11, located adjacent and in the opposite orientation to a well-characterized viral IL-6 gene. Northern blot analysis reveals that ORF11 is expressed during the KSHV lytic cycle with delayed-early transcription kinetics. We have determined the 5{prime} and 3{prime} untranslated region of the unspliced ORF11 transcript and identified both the transcription start site and the transcription termination site. Core promoter region, representing ORF11 promoter activity, was mapped to a 159nt fragment 5{prime} most proximal to the transcription start site. A functional TATA box was identified in the core promoter region. Interestingly, we found that ORF11 transcriptional activation is not responsive to Rta, the KSHV lytic switch protein. We also discovered that part of the ORF11 promoter region, the 209nt fragment upstream of the transcription start site, was repressed by phorbol esters. Our data help to understand transcription regulation of ORF11 and to elucidate roles of ORF11 in KSHV pathogenesis and life cycle.

  5. Repeat associated non-ATG translation initiation: one DNA, two transcripts, seven reading frames, potentially nine toxic entities!

    Directory of Open Access Journals (Sweden)

    Christopher E Pearson

    2011-03-01

    Full Text Available Diseases associated with unstable repetitive elements in the DNA, RNA, and amino acids have consistently revealed scientific surprises. Most diseases are caused by expansions of trinucleotide repeats, which ultimately lead to diseases like Huntington's disease, myotonic dystrophy, fragile X syndrome, and a series of spinocerebellar ataxias. These repeat mutations are dynamic, changing through generations and within an individual, and the repeats can be bi-directionally transcribed. Unsuspected modes of pathogenesis involve aberrant loss of protein expression; aberrant over-expression of non-mutant proteins; toxic-gain-of-protein function through expanded polyglutamine tracts that are encoded by expanded CAG tracts; and RNA-toxic-gain-of-function caused by transcripts harboring expanded CUG, CAG, or CGG tracts. A recent advance reveals that RNA transcripts with expanded CAG repeats can be translated in the complete absence of a starting ATG, and this Repeat Associated Non-ATG translation (RAN-translation occurs across expanded CAG repeats in all reading frames (CAG, AGC, and GCA to produce homopolymeric proteins of long polyglutamine, polyserine, and polyalanine tracts. Expanded CTG tracts expressing CUG transcripts also show RAN-translation occurring in all three frames (CUG, UGC, and GCU, to produce polyleucine, polycysteine, and polyalanine. These RAN-translation products can be toxic. Thus, one unstable (CAG•(CTG DNA can produce two expanded repeat transcripts and homopolymeric proteins with reading frames (the AUG-directed polyGln and six RAN-translation proteins, yielding a total of potentially nine toxic entities. The occurrence of RAN-translation in patient tissues expands our horizons of modes of disease pathogenesis. Moreover, since RAN-translation counters the canonical requirements of translation initiation, many new questions are now posed that must be addressed. This review covers RAN-translation and some of the pertinent

  6. Global proteogenomic analysis of human MHC class I-associated peptides derived from non-canonical reading frames.

    Science.gov (United States)

    Laumont, Céline M; Daouda, Tariq; Laverdure, Jean-Philippe; Bonneil, Éric; Caron-Lizotte, Olivier; Hardy, Marie-Pierre; Granados, Diana P; Durette, Chantal; Lemieux, Sébastien; Thibault, Pierre; Perreault, Claude

    2016-01-01

    In view of recent reports documenting pervasive translation outside of canonical protein-coding sequences, we wished to determine the proportion of major histocompatibility complex (MHC) class I-associated peptides (MAPs) derived from non-canonical reading frames. Here we perform proteogenomic analyses of MAPs eluted from human B cells using high-throughput mass spectrometry to probe the six-frame translation of the B-cell transcriptome. We report that ∼ 10% of MAPs originate from allegedly noncoding genomic sequences or exonic out-of-frame translation. The biogenesis and properties of these 'cryptic MAPs' differ from those of conventional MAPs. Cryptic MAPs come from very short proteins with atypical C termini, and are coded by transcripts bearing long 3'UTRs enriched in destabilizing elements. Relative to conventional MAPs, cryptic MAPs display different MHC class I-binding preferences and harbour more genomic polymorphisms, some of which are immunogenic. Cryptic MAPs increase the complexity of the MAP repertoire and enhance the scope of CD8 T-cell immunosurveillance. PMID:26728094

  7. Novel gene and gene model detection using a whole genome open reading frame analysis in proteomics

    OpenAIRE

    Fermin, Damian; Allen, Baxter B; Blackwell, Thomas W; Menon, Rajasree; Adamski, Marcin; Xu, Yin; Ulintz, Peter; Omenn, Gilbert S.; States, David J.

    2006-01-01

    Background Defining the location of genes and the precise nature of gene products remains a fundamental challenge in genome annotation. Interrogating tandem mass spectrometry data using genomic sequence provides an unbiased method to identify novel translation products. A six-frame translation of the entire human genome was used as the query database to search for novel blood proteins in the data from the Human Proteome Organization Plasma Proteome Project. Because this target database is ord...

  8. Frameless Stereotactic Radiosurgery, a Feasible Alternative to the Frame-Based Technique for the Treatment of Refractory Trigeminal Neuralgia.

    Science.gov (United States)

    Graff, Andrew E; Thomas, Andrew S; Reed, Aaron D; Skinner, William K

    2016-01-01

    Classic trigeminal neuralgia (TN) causes severe facial pain. Several treatment options exist for classic TN refractory to medical therapy, including stereotactic radiosurgery (SRS). Most studies in the medical literature used a frame-based SRS technique. Improvements in linear accelerator-based treatment systems and image guidance have led to the use of frameless SRS as a safe and feasible alternative to the frame-based technique for the treatment of refractory TN. We present a case of refractory TN successfully treated with frameless SRS. PMID:27186453

  9. Combination Antisense Treatment for Destructive Exon Skipping of Myostatin and Open Reading Frame Rescue of Dystrophin in Neonatal mdx Mice.

    Science.gov (United States)

    Lu-Nguyen, Ngoc B; Jarmin, Susan A; Saleh, Amer F; Popplewell, Linda; Gait, Michael J; Dickson, George

    2015-08-01

    The fatal X-linked Duchenne muscular dystrophy (DMD), characterized by progressive muscle wasting and muscle weakness, is caused by mutations within the DMD gene. The use of antisense oligonucleotides (AOs) modulating pre-mRNA splicing to restore the disrupted dystrophin reading frame, subsequently generating a shortened but functional protein has emerged as a potential strategy in DMD treatment. AO therapy has recently been applied to induce out-of-frame exon skipping of myostatin pre-mRNA, knocking-down expression of myostatin protein, and such an approach is suggested to enhance muscle hypertrophy/hyperplasia and to reduce muscle necrosis. Within this study, we investigated dual exon skipping of dystrophin and myostatin pre-mRNAs using phosphorodiamidate morpholino oligomers conjugated with an arginine-rich peptide (B-PMOs). Intraperitoneal administration of B-PMOs was performed in neonatal mdx males on the day of birth, and at weeks 3 and 6. At week 9, we observed in treated mice (as compared to age-matched, saline-injected controls) normalization of muscle mass, a recovery in dystrophin expression, and a decrease in muscle necrosis, particularly in the diaphragm. Our data provide a proof of concept for antisense therapy combining dystrophin restoration and myostatin inhibition for the treatment of DMD. PMID:25959011

  10. Efficient Restoration of the Dystrophin Gene Reading Frame and Protein Structure in DMD Myoblasts Using the CinDel Method.

    Science.gov (United States)

    Iyombe-Engembe, Jean-Paul; Ouellet, Dominique L; Barbeau, Xavier; Rousseau, Joël; Chapdelaine, Pierre; Lagüe, Patrick; Tremblay, Jacques P

    2016-01-01

    The CRISPR/Cas9 system is a great revolution in biology. This technology allows the modification of genes in vitro and in vivo in a wide variety of living organisms. In most Duchenne muscular dystrophy (DMD) patients, expression of dystrophin (DYS) protein is disrupted because exon deletions result in a frame shift. We present here the CRISPR-induced deletion (CinDel), a new promising genome-editing technology to correct the DMD gene. This strategy is based on the use of two gRNAs targeting specifically exons that precede and follow the patient deletion in the DMD gene. This pair of gRNAs induced a precise large additional deletion leading to fusion of the targeted exons. Using an adequate pair of gRNAs, the deletion of parts of these exons and the intron separating them restored the DMD reading frame in 62% of the hybrid exons in vitro in DMD myoblasts and in vivo in electroporated hDMD/mdx mice. Moreover, adequate pairs of gRNAs also restored the normal spectrin-like repeat of the dystrophin rod domain; such restoration is not obtained by exon skipping or deletion of complete exons. The expression of an internally deleted DYS protein was detected following the formation of myotubes by the unselected, treated DMD myoblasts. Given that CinDel induces permanent reparation of the DMD gene, this treatment would not have to be repeated as it is the case for exon skipping induced by oligonucleotides. PMID:26812655

  11. A noiseless kilohertz frame rate imaging detector based on microchannel plates read out with the Medipix2 CMOS pixel chip

    CERN Document Server

    Mikulec, Bettina; Ferrère, Didier; La Marra, Daniel; McPhate, J B; Tremsin, A S; Siegmund, O H W; Vallerga, J V; Clement, J; Ponchut, C; Rigal, J M; CERN. Geneva

    2006-01-01

    A new hybrid optical imaging detector is described that is being developed for the next generation adaptive optics (AO) wavefront sensors (WFS) for ground-based telescopes. The detector consists of a photocathode and proximity focused microchannel plates (MCPs) read out by the Medipix2 CMOS pixel ASIC. Each pixel of the Medipix2 device measures 55x55 um2 and comprises pre-amplifier, a window discriminator and a 14-bit counter. The 256x256 Medipix2 array can be read out noiselessly in 287 us. The readout can be electronically shuttered down to a temporal window of a few us. The Medipix2 is buttable on 3 sides to produce 512x(n*256) pixel devices. Measurements with ultraviolet light yield a spatial resolution of the detector at the Nyquist limit. Sub-pixel resolution can be achieved using centroiding algorithms. For the AO application, very high continuous frame rates of the order of 1 kHz are required for a matrix of 512x512 pixels. The design concepts of a parallel readout board are presented that will allow ...

  12. A noiseless kilohertz frame rate imaging detector based on microchannel plates read out with the Medipix2 CMOS pixel chip

    International Nuclear Information System (INIS)

    A new hybrid optical imaging detector is described that is being developed for the next generation adaptive optics (AO) wavefront sensors (WFS) for ground-based telescopes. The detector consists of a photocathode and proximity focused microchannel plates (MCPs) read out by the Medipix2 CMOS pixel ASIC. Each pixel of the Medipix2 device measures 55x55μm2 and comprises pre-amplifier, a window discriminator and a 14-bit counter. The 256x256 Medipix2 array can be read out noiselessly in 287μs. The readout can be electronically shuttered down to a temporal window of a few μs. The Medipix2 is buttable on three sides to produce 512x(n*256) pixel devices. Measurements with ultraviolet light yield a spatial resolution of the detector at the Nyquist limit. Sub-pixel resolution can be achieved using centroiding algorithms. For the AO application, very high continuous frame rates of the order of 1kHz are required for a matrix of 512x512 pixels. The design concepts of a parallel readout board are presented that will allow this fast data throughput. The development status of the optical WFS tube is also explained

  13. Human Cytomegalovirus UL138 Open Reading Frame Is Highly Conserved in Clinical Strains

    Institute of Scientific and Technical Information of China (English)

    Ying Qi; Rong He; Yan-ping Ma; Zheng-rong Sun; Yao-hua Ji; Qiang Ruan

    2009-01-01

    To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading flame (ORF) in clinical strains.Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains.Results UL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities of UL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleotide mutations were substitutions. The spatial structure and post-translational modification sites of UL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV UL138 sequence variations were not definitely related with different clinical symptoms.Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.

  14. ORFer – retrieval of protein sequences and open reading frames from GenBank and storage into relational databases or text files

    Directory of Open Access Journals (Sweden)

    Sievert Volker

    2002-12-01

    Full Text Available Abstract Background Functional genomics involves the parallel experimentation with large sets of proteins. This requires management of large sets of open reading frames as a prerequisite of the cloning and recombinant expression of these proteins. Results A Java program was developed for retrieval of protein and nucleic acid sequences and annotations from NCBI GenBank, using the XML sequence format. Annotations retrieved by ORFer include sequence name, organism and also the completeness of the sequence. The program has a graphical user interface, although it can be used in a non-interactive mode. For protein sequences, the program also extracts the open reading frame sequence, if available, and checks its correct translation. ORFer accepts user input in the form of single or lists of GenBank GI identifiers or accession numbers. It can be used to extract complete sets of open reading frames and protein sequences from any kind of GenBank sequence entry, including complete genomes or chromosomes. Sequences are either stored with their features in a relational database or can be exported as text files in Fasta or tabulator delimited format. The ORFer program is freely available at http://www.proteinstrukturfabrik.de/orfer. Conclusion The ORFer program allows for fast retrieval of DNA sequences, protein sequences and their open reading frames and sequence annotations from GenBank. Furthermore, storage of sequences and features in a relational database is supported. Such a database can supplement a laboratory information system (LIMS with appropriate sequence information.

  15. Genomic interspecies microarray hybridization: rapid discovery of three thousand genes in the maize endophyte, Klebsiella pneumoniae 342, by microarray hybridization with Escherichia coli K-12 open reading frames.

    Science.gov (United States)

    Dong, Y; Glasner, J D; Blattner, F R; Triplett, E W

    2001-04-01

    In an effort to efficiently discover genes in the diazotrophic endophyte of maize, Klebsiella pneumoniae 342, DNA from strain 342 was hybridized to a microarray containing 96% (n = 4,098) of the annotated open reading frames from Escherichia coli K-12. Using a criterion of 55% identity or greater, 3,000 (70%) of the E. coli K-12 open reading frames were also found to be present in strain 342. Approximately 24% (n = 1,030) of the E. coli K-12 open reading frames are absent in strain 342. For 1.6% (n = 68) of the open reading frames, the signal was too low to make a determination regarding the presence or absence of the gene. Genes with high identity between the two organisms are those involved in energy metabolism, amino acid metabolism, fatty acid metabolism, cofactor synthesis, cell division, DNA replication, transcription, translation, transport, and regulatory proteins. Functions that were less highly conserved included carbon compound metabolism, membrane proteins, structural proteins, putative transport proteins, cell processes such as adaptation and protection, and central intermediary metabolism. Open reading frames of E. coli K-12 with little or no identity in strain 342 included putative regulatory proteins, putative chaperones, surface structure proteins, mobility proteins, putative enzymes, hypothetical proteins, and proteins of unknown function, as well as genes presumed to have been acquired by lateral transfer from sources such as phage, plasmids, or transposons. The results were in agreement with the physiological properties of the two strains. Whole genome comparisons by genomic interspecies microarray hybridization are shown to rapidly identify thousands of genes in a previously uncharacterized bacterial genome provided that the genome of a close relative has been fully sequenced. This approach will become increasingly more useful as more full genome sequences become available. PMID:11282649

  16. Who needs RDD? Combining directory listings with cell phone exchanges for an alternative telephone sampling frame

    OpenAIRE

    Guterbock, Thomas; Diop, Abdoulaye; Ellis, James; Trung Le, Kien

    2011-01-01

    Abstract The traditional Random Digit Dialing method (list-assisted RDD using a frame of landline phone numbers) is clearly under threat. The difficulty and costs of completing telephone surveys have increased due to rising rates of refusal and non-contact. The completeness of coverage of list-assisted RDD samples has diminished due to the proliferation of cell-phone only households. The ability of list-assisted RDD to capture young, mobile, unmarried, and minority households is thus dimin...

  17. A novel sweet potato potyvirus open reading frame (ORF) is expressed via polymerase slippage and suppresses RNA silencing

    Science.gov (United States)

    Untiveros, Milton; Olspert, Allan; Artola, Katrin

    2016-01-01

    Summary The single‐stranded, positive‐sense RNA genome of viruses in the genus Potyvirus encodes a large polyprotein that is cleaved to yield 10 mature proteins. The first three cleavage products are P1, HCpro and P3. An additional short open reading frame (ORF), called pipo, overlaps the P3 region of the polyprotein ORF. Four related potyviruses infecting sweet potato (Ipomoea batatas) are predicted to contain a third ORF, called pispo, which overlaps the 3′ third of the P1 region. Recently, pipo has been shown to be expressed via polymerase slippage at a conserved GA6 sequence. Here, we show that pispo is also expressed via polymerase slippage at a GA6 sequence, with higher slippage efficiency (∼5%) than at the pipo site (∼1%). Transient expression of recombinant P1 or the ‘transframe’ product, P1N‐PISPO, in Nicotiana benthamiana suppressed local RNA silencing (RNAi), but only P1N‐PISPO inhibited short‐distance movement of the silencing signal. These results reveal that polymerase slippage in potyviruses is not limited to pipo expression, but can be co‐opted for the evolution and expression of further novel gene products. PMID:26757490

  18. Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames

    Indian Academy of Sciences (India)

    Parmit K Singh; Srividhya V Iyer; T Naga Sowjanya; B Kranthi Raj; Durgadas P Kasbekar

    2010-12-01

    In Neurospora crassa, crosses between normal sequence strains and strains bearing some translocations can yield progeny bearing a duplication (Dp) of the translocated chromosome segment. Here, 30 breakpoint junction sequences of 12 Dp-generating translocations were determined. The breakpoints disrupted 13 genes (including predicted genes), and created 10 novel open reading frames. Insertion of sequences from LG III into LG I as translocation T(UK818) disrupts the eat-3 gene, which is the ortholog of the Podospora anserine gene ami1. Since ami1-homozygous Podospora crosses were reported to increase the frequency of repeat-induced point mutation (RIP), we performed crosses homozygous for a deficiency in eat-3 to test for a corresponding increase in RIP frequency. However, our results suggested that, unlike in Podospora, the eat-3 gene might be essential for ascus development in Neurospora. Duplication–heterozygous crosses are generally barren in Neurospora; however, by using molecular probes developed in this study, we could identify Dp segregants from two different translocation–heterozygous crosses, and using these we found that the barren phenotype of at least some duplication–heterozygous crosses was incompletely penetrant.

  19. Framing the frame

    Directory of Open Access Journals (Sweden)

    Todd McElroy

    2007-08-01

    Full Text Available We examined how the goal of a decision task influences the perceived positive, negative valence of the alternatives and thereby the likelihood and direction of framing effects. In Study 1 we manipulated the goal to increase, decrease or maintain the commodity in question and found that when the goal of the task was to increase the commodity, a framing effect consistent with those typically observed in the literature was found. When the goal was to decrease, a framing effect opposite to the typical findings was observed whereas when the goal was to maintain, no framing effect was found. When we examined the decisions of the entire population, we did not observe a framing effect. In Study 2, we provided participants with a similar decision task except in this situation the goal was ambiguous, allowing us to observe participants' self-imposed goals and how they influenced choice preferences. The findings from Study 2 demonstrated individual variability in imposed goal and provided a conceptual replication of Study 1. %need keywords

  20. AN ALTERNATIVE APPROACH FOR TEACHER EDUCATION COURSES FRAMED BY A COLLABORATE PARTNERSHIP

    DEFF Research Database (Denmark)

    Pontoppidan, Birgitte

    2010-01-01

    The study presents an alternative didactical approach to teacher education linking practice and theory through a collaborative partnership setting. Using a “small scale” teaching design in which students alternate between schools and college it was possible to show some evidence that, by following...... traditional teacher education on its head and begins with a focus on practice so students alternate between school–based and college–based teaching in a cyclical fashion, and are encouraged to link theory with practice. This kind of college teaching demands a new teacher educational paradigm for which...... collaboration between schools and colleges is paramount. This study indicates that a collaborative partnership setting involving college and S&T- profile schools can provide a “room for study”, and that this kind of teaching design enhances the opportunities for students’ to develop teacher knowledge (aspects...

  1. An alternative approach for teacher education framed by a collaborative partnership setting

    DEFF Research Database (Denmark)

    Pontoppidan, Birgitte Schou

    2010-01-01

    The study presents an alternative didactical approach to teacher education linking practice and theory through a collaborative partnership setting. Using a ―small scale teaching design in which students alternate between schools and college it was possible to show someevidence that, by following...... this approach, first year student teachers in a science & technology class developed teacher knowledge (as aspects of PCK). The study identifies an example using Co-Re and PaPeR as a Resource Folio to show where evidence of developing teacher knowledge is seen. This didactical approach turns the...... traditional teacher education on its head and begins with a focus on practice so students alternate between school–based and college–based teaching in a cyclical fashion, and are encouraged to link theory with practice. This kind of college teaching demands a new teacher educational paradigm for which...

  2. Conserved-peptide upstream open reading frames (CPuORFs are associated with regulatory genes in angiosperms

    Directory of Open Access Journals (Sweden)

    Richard A Jorgensen

    2012-08-01

    Full Text Available Upstream open reading frames (uORFs are common in eukaryotic transcripts, but those that encode conserved peptides (CPuORFs occur in less than 1% of transcripts. The peptides encoded by three plant CPuORF families are known to control translation of the downstream ORF in response to a small signal molecule (sucrose, polyamines and phosphocholine. In flowering plants, transcription factors are statistically over-represented among genes that possess CPuORFs, and in general it appeared that many CPuORF genes also had other regulatory functions, though the significance of this suggestion was uncertain (Hayden and Jorgensen, 2007. Five years later the literature provides much more information on the functions of many CPuORF genes. Here we reassess the functions of 27 known CPuORF gene families and find that 22 of these families play a variety of different regulatory roles, from transcriptional control to protein turnover, and from small signal molecules to signal transduction kinases. Clearly then, there is indeed a strong association of CPuORFs with regulatory genes. In addition, 16 of these families play key roles in a variety of different biological processes. Most strikingly, the core sucrose response network includes three different CPuORFs, creating the potential for sophisticated balancing of the network in response to three different molecular inputs. We propose that the function of most CPuORFs is to modulate translation of a downstream major ORF (mORF in response to a signal molecule recognized by the conserved peptide and that because the mORFs of CPuORF genes generally encode regulatory proteins, many of them centrally important in the biology of plants, CPuORFs play key roles in balancing such regulatory networks.

  3. Identification of the polypeptides encoded in the unassigned reading frames 2, 4, 4L, and 5 of human mitochondrial DNA

    International Nuclear Information System (INIS)

    In previous work, antibodies prepared against chemically synthesized peptides predicted from the DNA sequence were used to identify the polypeptides encoded in three of the eight unassigned reading frames (URFs) of human mitochondrial DNA (mtDNA). In the present study, this approach has been extended to other human mtDNA URFs. In particular, antibodies directed against the NH2-terminal octapeptide of the putative URF2 product specifically precipitated component 11 of the HeLa cell mitochondrial translation products, the reaction being inhibited by the specific peptide. Similarly, antibodies directed against the COOH-terminal nonapeptide of the putative URF4 product reacted specifically with components 4 and 5, and antibodies against a COOH-terminal heptapeptide of the presumptive URF4L product reacted specifically with component 26. Antibodies against the NH2-terminal heptapeptide of the putative product of URF5 reacted with component 1, but only to a marginal extent; however, the results of a trypsin fingerprinting analysis of component 1 point strongly to this component as being the authentic product of URF5. The polypeptide assignments to the mtDNA URFs analyzed here are supported by the relative electrophoretic mobilities of proteins 11, 4-5, 26, and 1, which are those expected for the molecular weights predicted from the DNA sequence for the products of URF2, URF4, URF4L, and URF5, respectively. With the present assignment, seven of the eight human mtDNA URFs have been shown to be expressed in HeLa cells

  4. Identification of Immunodominant Antigens by Probing a Whole Chlamydia trachomatis Open Reading Frame Proteome Microarray Using Sera from Immunized Mice ▿ †

    OpenAIRE

    Cruz-Fisher, Maria I.; Cheng, Chunmei; Sun, Guifeng; Pal, Sukumar; Teng, Andy; Molina, Douglas M.; Kayala, Matthew A.; Vigil, Adam; Baldi, Pierre; Felgner, Philip L.; Liang, Xiaowu; de la Maza, Luis M.

    2010-01-01

    Chlamydia trachomatis infections can lead to severe chronic complications, including trachoma, ectopic pregnancy, and infertility. The only effective approach to disease control is vaccination. The goal of this work was to identify new potential vaccine candidates through a proteomics approach. We constructed a protein chip array (Antigen Discovery, Inc.) by expressing the open reading frames (ORFs) from C. trachomatis mouse pneumonitis (MoPn) genomic and plasmid DNA and tested it with serum ...

  5. The urease structural gene ureA in Rhizobium meliloti is preceded by an open reading frame necessary for urease activity.

    Science.gov (United States)

    Miksch, G

    1994-12-01

    An open reading frame (ORF1) located upstream of the urease structural gene ureA in Rhizobium meliloti strain AK631 was cloned and characterized by DNA sequencing. Comparison of the amino acid sequence revealed partial homology with the urease accessory gene ureD of Klebsiella aerogenes and Proteus mirabilis. Mutational analysis of ORF1 showed that the gene is necessary for urease activity. Its function is still unknown. PMID:7813887

  6. Kaposi's Sarcoma-Associated Herpesvirus Open Reading Frame 50/Rta Protein Activates the Entire Viral Lytic Cycle in the HH-B2 Primary Effusion Lymphoma Cell Line†

    OpenAIRE

    Gradoville, Lyndle; Gerlach, Jennifer; Grogan, Elizabeth; Shedd, Duane; Nikiforow, Sarah; Metroka, Craig; Miller, George

    2000-01-01

    Rta, the gene product of Kaposi's sarcoma-associated herpesvirus (KSHV) encoded mainly in open reading frame 50 (ORF50), is capable of activating expression of viral lytic cycle genes. What was not demonstrated in previous studies was whether KSHV Rta was competent to initiate the entire viral lytic life cycle including lytic viral DNA replication, late-gene expression with appropriate kinetics, and virus release. In HH-B2, a newly established primary effusion lymphoma (PEL) cell line, KSHV O...

  7. Alternative Framings, Countervailing Visions: Locating the "P" in Professional Identity Formation.

    Science.gov (United States)

    Hafferty, Frederic W; Michalec, Barret; Martimianakis, Maria Athina Tina; Tilburt, Jon C

    2016-02-01

    Professional identity formation in medical education is referenced increasingly as an object for educational reform. The authors introduce core concepts from two largely untapped literatures on identity and formation, contrasting framings on occupational preparation from within the organizational socialization literature with issues of socialization and professional acculturation from a military sciences perspective.The organizational sciences literature emphasizes socializing a workforce to "fit in," raising questions about how organization values might clash with core professional values concerning patient primary and social justice. The military literature, in turn, advances the notions of professional identity as a collective property, and that a particular social other (the public) must participate in shaping the group's identity as a profession.The authors extrapolate from these reviews that the training of physicians-as-professionals, and thus issues of socialization and identity formation, require intentionality and specificity around these contrasting issues. In turn, they argue that medical educators must attend to socializing trainees to a professional group identity while at the same time producing health care professionals who retain the capacity to resist the bureaucratic application of standardized solutions to contemporary problems. Educators must thus strive to identify the skills, knowledge, and attitudes necessary that will allow physicians-qua-professionals to function as a quasi-subversive work force and to disrupt the very system that helped to shape their identity, so that they may fulfill their mission to their patients. PMID:26535861

  8. A short insert in the leader sequence of RNA 3L, a long variant of Alfalfa mosaic virus RNA3, introduces two unidentified reading frames.

    Science.gov (United States)

    Jayasena, Kithsiri W; Randles, John W

    2004-12-01

    N20-RNA 3L, a large form of RNA 3 associated with Alfalfa mosaic virus (AMV) strain N20 comprises 2281 nt and has approximately 97% overall sequence similarity to the longest previously described RNA 3 of AMV strain YSMV (YSMV-RNA 3; 2188 nt). Compared with YSMV-RNA 3, N20-RNA 3L contains an additional 97 nt in the 5' leader upstream of the open reading frames for movement protein (MP) and coat protein (CP). Two overlapping unidentified reading frames (URF1 and URF2) result from this modification, each of which code for putative translation products of 21 amino acids. The URF1 putative peptide has a hydrophilic N-terminus and a hydrophobic C-terminus, indicating a possible association with both host cell membrane and cytosol whereas the putative URF2 product is predominantly hydrophobic. A further structural modification found in N20-RNA 3L is a new tandem repeat of 243 nts which overlaps with the MP open reading frame. PMID:15550770

  9. Differences in lower limb transverse plane joint moments during gait when expressed in two alternative reference frames.

    Science.gov (United States)

    Schache, Anthony G; Baker, Richard; Vaughan, Christopher L

    2007-01-01

    When comparing previous studies that have measured the three-dimensional moments acting about the lower limb joints (either external moments or opposing internal joint moments) during able-bodied adult gait, significant variation is apparent in the profiles of the reported transverse plane moments. This variation cannot be explained on the basis of adopted convention (i.e. external versus internal joint moment) or inherent variability in gait strategies. The aim of the current study was to determine whether in fact the frame in which moments are expressed has a dominant effect upon transverse plane moments and thus provides a valid explanation for the observed inconsistency in the literature. Kinematic and ground reaction force data were acquired from nine able-bodied adult subjects walking at a self-selected speed. Three-dimensional hip, knee and ankle joint moments during gait were calculated using a standard inverse dynamics approach. In addition to calculating internal joint moments, the components of the external moment occurring in the transverse plane at each of the lower limb joints were calculated to determine their independent effects. All moments were expressed in both the laboratory frame (LF) as well as the anatomical frame (AF) of the distal segment. With the exception of the ankle rotation moment in the foot AF, lower limb transverse plane joint moments during gait were found to display characteristic profiles that were consistent across subjects. Furthermore, lower limb transverse plane joint moments during gait differed when expressed in the distal segment AF compared to the LF. At the hip, the two alternative reference frames produced near reciprocal joint moment profiles. The components of the external moment revealed that the external ground reaction force moment was primarily responsible for this result. Lower limb transverse plane joint moments during gait were therefore found to be highly sensitive to a change in reference frame. These

  10. Adenovirus encoding XAF-1 and TNF‑α in the same open reading frame efficiently inhibits hepatocellular cancer cells.

    Science.gov (United States)

    Li, Kai; Li, Xinhong; Wu, Zhongjun; Zheng, Liansheng; Cui, Yuqin; Wang, Jun; Huang, Yin; Yan, Zhihong

    2016-06-01

    X‑linked inhibitor of apoptosis (XIAP)‑associated factor 1 (XAF‑1), a tumor suppressor, is downregulated in most human malignant tumors. However, the tumor suppressive role of XAF‑1 in hepatocellular carcinoma (HCC) and its therapeutic value require further elucidation. The present study examined the expression of XAF‑1 at the mRNA and protein level in the HCC and paired peritumor tissue specimens, as well as in HCC cell lines and a normal liver cell line. A recombinant adenovirus which co‑expressed XAF‑1 and TNF‑α was then constructed, and its effects on the proliferation and colony formation ability of the MHCC97H HCC cell line were assessed using apoptosis induction, flow cytometry, trypan blue staining assay and a clonogenic assay. The results demonstrated that the expression of XAF‑1 was significantly reduced in HCC tissues compared with that in their matched peritumor specimens, and a significant correlation with the tumor size, stage and tumor ‑ nodes ‑ metastasis stage was identified. The reduced levels of XAF‑1 were further confirmed the HCC cell lines MHCC97L, HepG2 and MHCC97H compared with those in the L02 normal liver cell line. The recombinant adenovirus Ad‑XAF‑1&TNF‑α, which co‑expressed XAF‑1 and TNF‑α, was shown to efficiently express the two proteins at the mRNA and protein level. Furthermore, infection with Ad‑XAF‑1&TNF‑α synergistically induced apoptosis, reduced the proliferation and colony formation ability of MHCC97L cells to a significantly greater extent than overexpression of XAF‑1 or TNF‑α individually. To the best of our knowledge, the present study was the first to construct an adenovirus which co‑expressed XAF‑1 and TNF‑α in the same open reading frame and expressed them proportionally. As Ad‑XAF‑1&TNF‑α inhibited HCC cells with enhanced efficiency, it may be applicable for the treatment of HCC. PMID:27121136

  11. Evolution of rbcL group IA introns and intron open reading frames within the colonial Volvocales (Chlorophyceae).

    Science.gov (United States)

    Nozaki, Hisayoshi; Takahara, Manabu; Nakazawa, Atsushi; Kita, Yoko; Yamada, Takashi; Takano, Hiroyoshi; Kawano, Shigeyuki; Kato, Masahiro

    2002-06-01

    Mobile group I introns sometimes contain an open reading frame (ORF) possibly encoding a site-specific DNA endonuclease. However, previous phylogenetic studies have not clearly deduced the evolutionary roles of the group I intron ORFs. In this paper, we examined the phylogeny of group IA2 introns inserted in the position identical to that of the chloroplast-encoded rbcL coding region (rbcL-462 introns) and their ORFs from 13 strains of five genera (Volvox, Pleodorina, Volvulina, Astrephomene, and Gonium) of the colonial Volvocales (Chlorophyceae) and a related unicellular green alga, Vitreochlamys. The rbcL-462 introns contained an intact or degenerate ORF of various sizes except for the Gonium multicoccum rbcL-462 intron. Partial amino acid sequences of some rbcL-462 intron ORFs exhibited possible homology to the endo/excinuclease amino acid terminal domain. The distribution of the rbcL-462 introns is sporadic in the phylogenetic trees of the colonial Volvocales based on the five chloroplast exon sequences (6021 bp). Phylogenetic analyses of the conserved intron sequences resolved that the G. multicoccum rbcL-462 intron had a phylogenetic position separate from those of other colonial volvocalean rbcL-462 introns, indicating the recent horizontal transmission of the intron in the G. multicoccum lineage. However, the combined data set from conserved intron sequences and ORFs from most of the rbcL-462 introns resolved robust phylogenetic relationships of the introns that were consistent with those of the host organisms. Therefore, most of the extant rbcL-462 introns may have been vertically inherited from the common ancestor of their host organisms, whereas such introns may have been lost in other lineages during evolution of the colonial Volvocales. In addition, apparently higher synonymous substitutions than nonsynonymous substitutions in the rbcL-462 intron ORFs indicated that the ORFs might evolve under functional constraint, which could result in homing of the

  12. Effects of a virtual platform in reading comprehension and vocabulary: An alternative to improve reading abilities in Elementary school

    OpenAIRE

    Cecilia Thorne; Kim Morla; Paola Ucelli; Teresa Nakano; Beatriz Mauchi; Lorena Landeo; Angie Vásquez; Román Huerta

    2013-01-01

    Given, on the one hand, the poor results obtained by Peruvian children in the national and international reading assessments. And on the other hand, the increased investment intechnology for schools in the country, this study aimed to develop and test an online tool to improve reading comprehension. In order to do this, the reading comprehension strategies and vocabulary activities from the research-based digital environment ICON were adapted to design the platform LEO. A total of 88 fifth gr...

  13. Production of Candida antaractica Lipase B Gene Open Reading Frame using Automated PCR Gene Assembly Protocol on Robotic Workcell & Expression in Ethanologenic Yeast for use as Resin-Bound Biocatalyst in Biodiesel Production

    Science.gov (United States)

    A synthetic Candida antarctica lipase B (CALB) gene open reading frame (ORF) for expression in yeast was produced using an automated PCR assembly and DNA purification protocol on an integrated robotic workcell. The lycotoxin-1 (Lyt-1) C3 variant gene ORF was added in-frame with the CALB ORF to pote...

  14. Effects of a virtual platform in reading comprehension and vocabulary: An alternative to improve reading abilities in Elementary school

    Directory of Open Access Journals (Sweden)

    Cecilia Thorne

    2013-06-01

    Full Text Available Given, on the one hand, the poor results obtained by Peruvian children in the national and international reading assessments. And on the other hand, the increased investment intechnology for schools in the country, this study aimed to develop and test an online tool to improve reading comprehension. In order to do this, the reading comprehension strategies and vocabulary activities from the research-based digital environment ICON were adapted to design the platform LEO. A total of 88 fifth graders from urban middle-to-low-income private schools from Lima participated in this quasi-experimental study, which involved acontrol group and a treatment group that participated in a 12-week teacher-mediated digital intervention. All participants were administered reading and vocabulary assessments pre and post intervention. Results revealed that students who participated in the intervention achieved higher comprehension scores for narrative texts and higher vocabulary scores than those of the control group.

  15. Reading comprehension as an alternative tool for teaching science and nuclear technology

    International Nuclear Information System (INIS)

    In recent decades, the vast amount of information originated in the production of knowledge and its applications, has highlighted the importance of being independent readers, critics, and able to interpret written material circulating referred to scientific and technological issues, that invade the people's daily life. Moreover, in the last stage of education system of all future citizens of the country, the results of many diagnoses have highlighted the difficulties of young students to understand the texts related to science and technology. However, simultaneously with these weaknesses, students permanently express the need to relate science and technology to everyday life, and are interested in the discussion of the news related to atomic energy spread by the mass media. This duality lack of interest in reading vs interest in knowledge in certain subjects, is what has been taken into account when proposing this pedagogical approach that simultaneously involves several aspects. From the need to find a trigger for the treatment of a particular issue, to familiarization of students with the vocabulary and methodology of science ill the debate on the characteristics of specific technological applications of nuclear technology. Considering particularly the last of these factors, since 2011 has been developed in Jose Maria Paz School of Cordoba, Reading Comprehension Experience, using texts with scientific and technological contents published by Institute for Energy and Development (IEDS) of the National Atomic Energy Commission (CNEA) in Knowledge Leaves Series, as a methodological tool, to bring students to the physics of the atom and matter. The reading strategy used is based on the hypothesis of the type of questions being asked about the contents, can help students to develop reading strategies for comprehension and thus contribute positively to his learning. With this proposal it has been observed an increased on student interest in learning natural science

  16. Redes alternativas de comunicación, framing y la construcción del poder político (Alternative communication networks, framing and construction of political power

    Directory of Open Access Journals (Sweden)

    Mayra Martínez Avidad

    2011-12-01

    Full Text Available El objetivo del presente artículo es analizar el papel de las redes alternativas de comunicación en la construcción del poder político a través del proceso de framing o enmarcado. En los últimos años, la capacidad del público para producir (nuestros propios mensajes que potencialmente desafíen los encuadres fijados por la elite política se ha expandido enormemente con la llegada de Internet y las comunicaciones móviles. En este sentido, las redes virtuales son importantes porque pueden aumentar la capacidad de las masas para comunicarse entre sí transformando los marcos de referencia individuales en marcos interpretativos de carácter colectivo.The aim of this paper is to analyze the role of alternative communication networks in the construction of political power through the process of framing. In recent years, the ability of the public to produce (our own messages that potentially challenge those frames established by political elites has been importantly expanded by the advent of Internet and mobile communications. In this sense, virtual networks are important because they can influence the capacity of masses to communicate with each other transforming individual frames of reference into collective interpretative frames.

  17. Slip of grip of a molecular motor on a crowded track: Modeling shift of reading frame of ribosome on RNA template

    CERN Document Server

    Mishra, Bhavya; Chowdhury, Debashish

    2016-01-01

    We develop a stochastic model for the programmed frameshift of ribosomes synthesizing a protein while moving along a mRNA template. Normally the reading frame of a ribosome decodes successive triplets of nucleotides on the mRNA in a step-by-step manner. We focus on the programmed shift of the ribosomal reading frame, forward or backward, by only one nucleotide which results in a fusion protein; it occurs when a ribosome temporarily loses its grip to its mRNA track. Special "slippery" sequences of nucleotides and also downstream secondary structures of the mRNA strand are believed to play key roles in programmed frameshift. Here we explore the role of an hitherto neglected parameter in regulating -1 programmed frameshift. Specifically, we demonstrate that the frameshift frequency can be strongly regulated also by the density of the ribosomes, all of which are engaged in simultaneous translation of the same mRNA, at and around the slippery sequence. Monte Carlo simulations support the analytical predictions obt...

  18. The CPT1C 5'UTR contains a repressing upstream open reading frame that is regulated by cellular energy availability and AMPK.

    Directory of Open Access Journals (Sweden)

    Ines Lohse

    Full Text Available BACKGROUND: Translational control is utilized as a means of regulating gene expression in many species. In most cases, posttranscriptional regulatory mechanisms play an important role in stress response pathways and can lead to dysfunctional physiology if blocked by mutations. Carnitine Palmitoyltransferase 1 C (CPT1C, the brain-specific member of the CPT 1 family, has previously been shown to be involved in regulating metabolism in situations of energy surplus. PRINCIPAL FINDINGS: Sequence analysis of the CPT1C mRNA revealed that it contains an upstream open reading frame (uORF in the 5' UTR of its mRNA. Using CPT1C 5' UTR/luciferase constructs, we investigated the role of the uORF in translational regulation. The results presented here show that translation from the CPT1C main open reading frame (mORF is repressed by the presence of the uORF, that this repression is relieved in response to specific stress stimuli, namely glucose deprivation and palmitate-BSA treatment, and that AMPK inhibition can relieve this uORF-dependent repression. SIGNIFICANCE: The fact that the mORF regulation is relieved in response to a specific set of stress stimuli rather than general stress response, hints at an involvement of CPT1C in cellular energy-sensing pathways and provides further evidence for a role of CPT1C in hypothalamic regulation of energy homeostasis.

  19. Using Simulations to Investigate the Longitudinal Stability of Alternative Schemes for Classifying and Identifying Children with Reading Disabilities

    Science.gov (United States)

    Schatschneider, Christopher; Wagner, Richard K.; Hart, Sara A.; Tighe, Elizabeth L.

    2016-01-01

    The present study employed data simulation techniques to investigate the 1-year stability of alternative classification schemes for identifying children with reading disabilities. Classification schemes investigated include low performance, unexpected low performance, dual-discrepancy, and a rudimentary form of constellation model of reading…

  20. Alternative Text Types to Improve Reading Fluency for Competent to Struggling Readers

    OpenAIRE

    Timothy V. Rasinski; William H. Rupley; David D. Paige; William Dee Nichols

    2016-01-01

    This article offers instructional suggestions and strategies based on research and theoretical literature for developing reading fluency through the use of rhyming poetry and other texts beyond the narrative and informational texts that have been traditionally used for reading instruction. Readers’ lack of fluency in reading can be a monumental impediment to proficiency in good comprehension and overall reading competency. For all readers it is well established that as they pro...

  1. Alternative Text Types to Improve Reading Fluency for Competent to Struggling Readers

    Directory of Open Access Journals (Sweden)

    Timothy V. Rasinski

    2016-01-01

    Full Text Available This article offers instructional suggestions and strategies based on research and theoretical literature for developing reading fluency through the use of rhyming poetry and other texts beyond the narrative and informational texts that have been traditionally used for reading instruction. Readers’ lack of fluency in reading can be a monumental impediment to proficiency in good comprehension and overall reading competency. For all readers it is well established that as they progress in reading competence their reading ability grows (Stanovich, 1993/1994. This continued reading success begets continued reading growth; however, many struggling readers have difficulty in moving to a level of automaticity and fluency in their reading that enables them to engage in a successful practice. Lack of practice inhibits their reading comprehension. Readers’ abilities to effectively comprehend texts are significantly affected by their proficiency in accurate and automatic word recognition and prosody (May, 1998; Stanovich, 1993/1994; LaBerge & Samuels, 1974; Schreiber, 1991. Repeated reading practice has been shown to be a powerful way to improve these important fluency competencies. Certain texts are particularly well suited for repeated reading that improves both aspects of fluency

  2. Alternative Text Types to Improve Reading Fluency for Competent to Struggling Readers

    Science.gov (United States)

    Rasinski, Timothy V.; Rupley, William H.; Pagie, David D.; Nichols, William Dee

    2016-01-01

    This article offers instructional suggestions and strategies based on research and theoretical literature for developing reading fluency through the use of rhyming poetry and other texts beyond the narrative and informational texts that have been traditionally used for reading instruction. Readers' lack of fluency in reading can be a monumental…

  3. Determination of the sequence of the complete open reading frame and the 5 ' NTR of the Paderborn isolate of classical swine fever virus

    DEFF Research Database (Denmark)

    Oleksiewicz, Martin B.; Rasmussen, Thomas Bruun; Normann, Preben;

    2003-01-01

    virtually identical, confirming that the Paderborn isolate triggered the Dutch outbreak, and furthermore showing that this single isolate was stable throughout the whole Dutch outbreak (the above reviewed in [C. Terpstra, A. J. de Smit, Veterinary Microbiol. 77 (2000) 3-15]). We determined the nucleotide...... sequence of the 5' NTR (by 5' RACE) and the complete open reading frame of the Paderborn isolate (GenBank AY072924). Our sequence was identical to previously published partial 5'NTR and E2 sequences for the index Paderborn 1997 and Dutch 1997 (Venhorst) isolates, confirming the identity of the virus we....... Paderborn is the only recent European CSFV field isolate for which a complete sequence is available, and given Paderborns genetic and antigenic uniqueness, the Paderborn sequence may have practical use for diagnostic and vaccine antigen development....

  4. Isolation and expression of human cytokine synthesis inhibitory factor cDNA clones: Homology to Epstein-Barr virus open reading frame BCRFI

    International Nuclear Information System (INIS)

    The authors demonstrated the existence of human cytokine synthesis inhibitory factor (DSIF) [interleukin 10 (IL-10)]. cDNA clones encoding human IL-10 (hIL-10) were isolated from a tetanus toxin-specific human T-cell clone. Like mouse IL-10, hIL-10 exhibits strong DNA and amino acid sequence homology to an open reading frame in the Epstein-Barr virus, BDRFL. hIL-10 and the BCRFI product inhibit cytokine synthesis by activated human peripheral blood mononuclear cells and by a mouse Th1 clone. Both hIL-10 and mouse IL-10 sustain the viability of a mouse mast cell line in culture, but BCRFI lacks comparable activity in this way, suggesting that BCRFI may have conserved only a subset of hIL-10 activities

  5. Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta

    Directory of Open Access Journals (Sweden)

    Nyalwidhe Julius

    2008-05-01

    Full Text Available Abstract Background Cryptophytes are highly compartmentalized organisms, expressing a secondary minimized eukaryotic genome in the nucleomorph and its surrounding remnant cytoplasm, in addition to the cell nucleus, the mitochondrion and the plastid. Because the members of the nucleomorph-encoded proteome may contribute to essential cellular pathways, elucidating nucleomorph-encoded functions is of utmost interest. Unfortunately, cryptophytes are inaccessible for genetic transformations thus far. Therefore the functions of nucleomorph-encoded proteins must be elucidated indirectly by application of methods in genetically accessible organisms. Results Orf222, one of the uncharacterized nucleomorph-specific open reading frames of the cryptophyte Guillardia theta, shows homology to slr1649 of Synechocystis sp. PCC 6803. Recently a further homolog from Synechococcus sp. PCC 7002 was characterized to encode a phycocyanin-β155-bilin lyase. Here we show by insertion mutagenesis that the Synechocystis sp. PCC 6803 slr1649-encoded protein also acts as a bilin lyase, and additionally contributes to linker attachment and/or stability of phycobilisomes. Finally, our results indicate that the phycocyanin-β155-bilin lyase of Synechocystis sp. PCC 6803 can be complemented in vivo by the nucleomorph-encoded open reading frame orf222. Conclusion Our data show that the loss of phycocyanin-lyase function causes pleiotropic effects in Synechocystis sp. PCC 6803 and indicate that after separating from a common ancestor protein, the phycoerythrin lyase from Guillardia theta has retained its capacity to couple a bilin group to other phycobiliproteins. This is a further, unexpected example of the universality of phycobiliprotein lyases.

  6. STEM strain analysis at sub-nanometre scale using millisecond frames from a direct electron read-out CCD camera

    International Nuclear Information System (INIS)

    We report on strain analysis by nano-beam electron diffraction with a spatial resolution of 0.5nm and a strain precision in the 4–7·10−4 range. Series of up to 160000 CBED patterns have been acquired in STEM mode with a semi-convergence angle of the incident probe of 2.6mrad, which enhances the spatial resolution by a factor of 5 compared to nearly parallel illumination. Firstly, we summarise 3 different algorithms to detect CBED disc positions accurately: selective edge detection and circle fitting, radial gradient maximisation and cross-correlation with masks. They yield equivalent strain profiles in growth direction for a stack of 5 InxGa1−xNyAs1−y/GaAs layers with tensile and compressive strain. Secondly, we use a direct electron read-out pnCCD detector with ultrafast readout hardware and a quantum efficiency close to 1 both to show that the same strain profiles are obtained at 200 times higher readout rates of 1kHz and to enhance strain precision to 3.5·10−4 by recording the weak 008 disc

  7. The Reading Response E-Journal: An Alternative Way to Engage Low-Achieving EFL Students

    Science.gov (United States)

    Lee, Hsiao-Chien

    2013-01-01

    The reading response journal has been valued as an effective tool for involving students in authentic reading and writing activities. As the internet has become an essential medium in today's English classroom, it is advisable to integrate both the web and the journal when approaching our EFL students. In order to motivate and engage my…

  8. Pair Frames

    OpenAIRE

    Fereydooni, Abolhassan; Safapour, Ahmad

    2011-01-01

    In this paper a new concept related to the frame theory is introduced; the notion of pair frame. By investigating some properties of such frames, it is shown that pair frames are a generalization of ordinary frames. Some classes of of them are introduced such as (p, q)-pair frames and near identity pair frames.

  9. Adenovirus E4 open reading frame 4-induced dephosphorylation inhibits E1A activation of the E2 promoter and E2F-1-mediated transactivation independently of the retinoblastoma tumor suppressor protein

    DEFF Research Database (Denmark)

    Mannervik, M; Fan, S; Ström, A C;

    1999-01-01

    viral E4 open reading frame 4 (E4-ORF4) protein. This effect does not to require the retinoblastoma protein that previously has been shown to regulate E2F activity. The inhibitory activity of E4-ORF4 appears to be specific because E4-ORF4 had little effect on, for example, E4-ORF6/7 transactivation of...

  10. Popcorn Story Frames.

    Science.gov (United States)

    DiLella, Carol Ann

    This paper presents "popcorn story frames"--holistic outlines that facilitate comprehension when reading and writing stories, useful for outlining stories read and for creating outlines for original student stories--that are particularly useful for elementary and intermediate school students. "Popcorn" pops in a horizontal manner rather than in a…

  11. 78 FR 27953 - Reorganization of Foreign-Trade Zone 147 Under Alternative Site Framework Reading, Pennsylvania

    Science.gov (United States)

    2013-05-13

    ... inviting public comment was given in the Federal Register (77 FR 66796-66797, 11/7/2012) and the... Reading, Pennsylvania Pursuant to its authority under the Foreign-Trade Zones Act of June 18, 1934,...

  12. Generation of Ips cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame

    Institute of Scientific and Technical Information of China (English)

    Lijian Shao; Wei Feng; Yan Sun; Hao Bai; Jun Liu; Caroline Currie; Jaejung Kim; Rafael Gama; Zack Wang; Zhijian Qian; Lucy Liaw; Wen-Shu Wu

    2009-01-01

    Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultane-ous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration sites in their ge-nomes. This can increase the probability of gene mutagenesis and genomic instability, and present significant barriers to both research and clinical application studies of iPS cells. In this paper, we present a simple lentivirus reprogram-ming system in which defined factors are fused in-frame into a single open reading frame (ORF) via self-cleaving 2A sequences. A GFP marker is placed downstream of the transgene to enable tracking of transgene expression. We demonstrate that this polycistronic expression system efficiently generates iPS cells. The generated iPS cells have nor-mal karyotypes and are similar to mouse embryonic stem cells in morphology and gene expression. Moreover, they can differentiate into cell types of the three embryonic germ layers in both in vitro and in vivo assays. Remarkably, most of these iPS cells only harbor a single copy of viral vector. This system provides a valuable tool for generation of iPS cells, and our data suggest that the balance of expression of transduced reprogramming TFs in each cell is essen-tial for the reprogramming process. More importantly, when delivered by non-integrating gene-delivery systems, this re-engineered single ORF will facilitate efficient generation of human iPS cells free of genetic modifications.

  13. Open reading frames 1a and 1b of the porcine reproductive and respiratory syndrome virus (PRRSV) collaboratively initiate viral minus-strand RNA synthesis.

    Science.gov (United States)

    Tang, Yan-Dong; Fang, Qiong-Qiong; Liu, Ji-Ting; Wang, Tong-Yun; Wang, Yu; Tao, Ye; Liu, Yong-Gang; Cai, Xue-Hui

    2016-09-01

    The porcine reproductive and respiratory syndrome virus (PRRSV) causes a persistent threat to the swine industry, especially when highly pathogenic PRRSV (HP-PRRSV) emerges. Previous studies have indicated that PRRSV RNA synthesis was correlated with HP-PRRSV virulence. PRRSV RNA synthesis includes genomic RNA and sub-genomic mRNA, and these processes require minus-strand RNA as a template. However, the mechanisms involved in PRRSV minus-strand RNA synthesis are not fully understood. A mini-genome system can be used to assess viral replication mechanisms and to evaluate the effects of potential antiviral drugs on viral replicase activities. In this study, we developed a mini-genome system that uses firefly luciferase as a reporter. Based on this system, we found that PRRSV RNA-dependent RNA polymerase nsp9 alone failed to activate virus minus-strand RNA synthesis. We also demonstrated that combinations of open reading frames 1a (ORF1a) and ORF1b are necessary for viral minus-strand RNA synthesis. PMID:27378424

  14. Expression, nucleotide sequence and mutational analysis of two open reading frames in the nif gene region of Anabaena sp. strain PCC7120.

    Science.gov (United States)

    Borthakur, D; Basche, M; Buikema, W J; Borthakur, P B; Haselkorn, R

    1990-04-01

    A 1.8 kb transcript corresponding to a region of the Anabaena 7120 chromosome 4 kb downstream of the nifHDK operon appears 12-18 h after heterocyst induction. The DNA corresponding to this transcript was sequenced and found to contain two open reading frames, designated ORF 1 and ORF 2. Two polypeptides, of 30 kDa and 13 kDa, encoded by these ORFs were expressed in Escherichia coli. An apparent start site for the transcript, detected by S1 nuclease protection, was located 42 bp upstream of the ATG start codon of ORF 1. ORF 2 shows strong sequence similarity to ORF 6 in the nif gene region of Azotobacter vinelandii. ORF 1 was interrupted using a 1.4 kb neomycin resistance cassette and the resulting mutant grew very slowly on medium lacking combined nitrogen. The mutant had 45% of wild-type acetylene reduction activity, which could be complemented by a 2.8 kb EcoRI fragment of wild-type Anabaena DNA containing only ORF 1 and ORF 2. Thus, one or both of these ORFs is required for efficient nitrogen fixation in Anabaena. PMID:2115111

  15. Genetic polymorphism of UL144 open reading frame of human cytomegalovirus DNA detected in colon samples from infants with Hirschsprung's disease

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    AIM: To explore the genetic diversities of UL144 open reading frame (ORF) of cytomegalovirus DNA detected in colon tissue from infants with Hirschsprung's disease (HD)by sequencing UL144 DNA in 23 aganglionic colon tissue and 4 urine samples from 25 HD infants.METHODS: Nest PCR was performed for amplification of the UL144 gene. The UL144 gene was analyzed with softwares, such as DNAclub, BioEdit, PROSITE database,and DNAstar.RESULTS: The strains from HD patients were distributed among three genotypes of UL144: group 1A (64%),group 2 (24%), and group 3 (12%). The UL144 genotypes between strains from HD and control group were compared by chi square test (χ2 = 1.870, P = 0.393).Strains from the colon were sporadically distributed in UL144 genotypes.CONCLUSION: There are genetic diversities of UL144ORF in colon tissue of infants with HD. However,cytomegalovirus UL144 genotypes are not associated with clinical manifestations of HD.

  16. Peer Tutoring for Reading Fluency as a Feasible and Effective Alternative in Response to Intervention Systems

    Science.gov (United States)

    Dufrene, Brad A.; Reisener, Carmen D.; Olmi, D. Joe; Zoder-Martell, Kimberly; McNutt, Marlena R.; Horn, Dana R.

    2010-01-01

    Peer tutoring is an evidence-based procedure for improving academic performance for a variety of skill areas. The current study evaluated the feasibility and impact of a peer tutoring package for reading fluency with 4 middle school students receiving Tier II remedial supports. This study used a multiple baseline design across participants to…

  17. Subfusion Frames

    OpenAIRE

    Amiri, Z.; Dehghan, M. A.; E Rahimi

    2012-01-01

    Fusion frames are generalizations of frames in Hilbert spaces which were introduced by Casazza et al. (2008). In the present paper, we study the relations between fusion frames and subfusion frame operators. Specially, we introduce new construction of subfusion frames and derive new results.

  18. Varicella-Zoster virus proteins encoded by open reading frames 14 and 67 are both dispensable for the establishment of latency in a rat model

    International Nuclear Information System (INIS)

    A rat model of Varicella-Zoster virus (VZV) provides a system in which to investigate the molecular determinants of viral latency in dorsal root ganglia (DRG). In this study, we determined whether the VZV glycoproteins gC and gI, corresponding to VZV open reading frames (ORFs) 14 and 67, respectively, were required for the establishment of latency in this model. A VZV gI deletion mutant (ΔgI) derived from a recombinant Oka (rOka) cosmid and a gC null mutant obtained from a clinical isolate were inoculated into the footpads of 6-week-old rats, and the presence of viral DNA and eight different VZV RNA transcripts corresponding to the three classes of genes was investigated by in situ RT-PCR amplification and in situ hybridization (ISH) in the DRG at 1 week, 1 month, and 18-24 months after infection. VZV DNA and restricted RNA expression was established with both deletion mutants as well as the parental rOka virus. Both VZV DNA and RNA were detected in neurons and non-neuronal cells. The pattern of viral RNA expression detected with both gC and gI mutants was restricted with transcripts for VZV genes 62 and 63 most frequently expressed 18-24 months after infection. Transcripts for VZV genes 18, 28, and 29 were also detected at these time points but at a slightly lower frequency. Transcripts for the late gene 40 were never detected. We conclude that VZV ORFs 14 and 67 are dispensable for the establishment of a latent infection in this model

  19. A Short Open Reading Frame Encompassing the MicroRNA173 Target Site Plays a Role in trans-Acting Small Interfering RNA Biogenesis.

    Science.gov (United States)

    Yoshikawa, Manabu; Iki, Taichiro; Numa, Hisataka; Miyashita, Kyoko; Meshi, Tetsuo; Ishikawa, Masayuki

    2016-05-01

    trans-Acting small interfering RNAs (tasiRNAs) participate in the regulation of organ morphogenesis and determination of developmental timing in plants by down-regulating target genes through mRNA cleavage. The production of tasiRNAs is triggered by microRNA173 (miR173) and other specific microRNA-mediated cleavage of 5'-capped and 3'-polyadenylated primary TAS transcripts (pri-TASs). Although pri-TASs are not thought to encode functional proteins, they contain multiple short open reading frames (ORFs). For example, the primary TAS2 transcript (pri-TAS2) contains 11 short ORFs, and the third ORF from the 5' terminus (ORF3) encompasses the miR173 target site. Here, we show that nonsense mutations in ORF3 of pri-TAS2 upstream of the miR173 recognition site suppress tasiRNA accumulation and that ORF3 is translated in vitro. Glycerol gradient centrifugation analysis of Arabidopsis (Arabidopsis thaliana) plant extracts revealed that pri-TAS2 and its miR173-cleaved 5' and 3' fragments are fractionated together in the polysome fractions. These and previous results suggest that the 3' fragment of pri-TAS2, which is a source of tasiRNAs, forms a huge complex containing SGS3, miR173-programmed AGO1 RNA-induced silencing complex, the 5' fragment, and ribosomes. This complex overaccumulated, moderately accumulated, and did not accumulate in rdr6, sde5, and sgs3 mutants, respectively. The sgs3 sde5 and rdr6 sde5 double mutants showed phenotypes similar to those of sgs3 and sde5 single mutants, respectively, with regard to the TAS2-related RNA accumulation, suggesting that the complex is formed in an SGS3-dependent manner, somehow modified and stabilized by SDE5, and becomes competent for RDR6 action. Ribosomes in this complex likely play an important role in this process. PMID:26966170

  20. Popcorn Story Frames from a Multicultural Perspective.

    Science.gov (United States)

    DiLella, Carol Ann

    Popcorn story frames from a multicultural perspective are holistic outlines that in the reading/writing process facilitate comprehension for all cultures learning to read and write stories. Popcorn story frames are structured and modeled in a horizontal fashion just like popcorn pops in a horizontal fashion. The frames are designed for learners…

  1. Timber frame walls

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place; Brandt, Erik

    2010-01-01

    A ventilated cavity is usually considered good practice for removing moisture behind the cladding of timber framed walls. Timber frame walls with no cavity are a logical alternative as they are slimmer and less expensive to produce and besides the risk of a two-sided fire behind the cladding is...

  2. Corporal Punishment in American Education: Readings in History, Practice, and Alternatives.

    Science.gov (United States)

    Hyman, Irwin A., Ed; Wise, James H., Ed.

    Virtually all of the essays in this collection reach the conclusion that corporal punishment in the schools is counterproductive. The book is organized into sections presenting historical, psychological, and legal perspectives on this subject, as well as case studies, opinion surveys, and the results of research. Rational alternatives to the use…

  3. Mechanical Color Reading of Wood-Staining Fungal Pigment Textile Dyes: An Alternative Method for Determining Colorfastness

    Directory of Open Access Journals (Sweden)

    Eric M. Hinsch

    2016-07-01

    Full Text Available Colorfastness to washing and crocking (color loss due to rubbing are essential qualities for any dye/fabric combination that will be used for garments or upholstery. In this study, colorfastness to washing and crocking of fabrics dyed with wood-staining fungal pigments was compared to colorfastness of commercial dyes using an alternative mechanical testing method. Overall, wood-staining fungal pigments out performed commercial dyes for colorfastness to washing and wet and dry crocking. Xylindein was the most colorfast dye. Draconin red yielded inconsistent results, and the yellow pigment required a mordant to achieve any colorfastness. This study showed that the mechanical color reading method, along with statistical analysis, provided an objective, repeatable gauge of colorfastness, although visual inspection is also needed for practical purposes.

  4. Integer Frames

    OpenAIRE

    Casazza, Peter G.; Lynch, Richard G.; Tremain, Janet C.; Woodland, Lindsey M.

    2013-01-01

    Finite frame theory has become a powerful tool for many applications of mathematics. In this paper we introduce a new area of research in frame theory: Integer frames. These are frames having all integer coordinates with respect to a fixed orthonormal basis for a Hilbert space. Integer frames have potential to mitigate quantization errors and transmission losses as well as speeding up computation times. This paper gives the first systematic study of this important class of finite Hilbert spac...

  5. Framing politics

    OpenAIRE

    Vreese, de, C.H.; Lecheler, S.K.

    2010-01-01

    This dissertation supplies a number of research findings that add to a theory of news framing effects, and also to the understanding of the role media effects play in political communication. We show that researchers must think more about what actually constitutes a framing effect, and that a dissociation of framing from other media effects concepts is not necessarily the ideal way to go in creating a more advanced framing theory. We also provide the first contours of a theory of news framing...

  6. Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell counting

    Directory of Open Access Journals (Sweden)

    Gunetti Monica

    2012-05-01

    Full Text Available Abstract Background The quality and safety of advanced therapy products must be maintained throughout their production and quality control cycle to ensure their final use in patients. We validated the cell count method according to the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use and European Pharmacopoeia, considering the tests’ accuracy, precision, repeatability, linearity and range. Methods As the cell count is a potency test, we checked accuracy, precision, and linearity, according to ICH Q2. Briefly our experimental approach was first to evaluate the accuracy of Fast Read 102® compared to the Bürker chamber. Once the accuracy of the alternative method was demonstrated, we checked the precision and linearity test only using Fast Read 102®. The data were statistically analyzed by average, standard deviation and coefficient of variation percentages inter and intra operator. Results All the tests performed met the established acceptance criteria of a coefficient of variation of less than ten percent. For the cell count, the precision reached by each operator had a coefficient of variation of less than ten percent (total cells and under five percent (viable cells. The best range of dilution, to obtain a slope line value very similar to 1, was between 1:8 and 1:128. Conclusions Our data demonstrated that the Fast Read 102® count method is accurate, precise and ensures the linearity of the results obtained in a range of cell dilution. Under our standard method procedures, this assay may thus be considered a good quality control method for the cell count as a batch release quality control test. Moreover, the Fast Read 102® chamber is a plastic, disposable device that allows a number of samples to be counted in the same chamber. Last but not least, it overcomes the problem of chamber washing after use and so allows a cell count in a clean environment such as that in a

  7. Sparse Matrices in Frame Theory

    DEFF Research Database (Denmark)

    Lemvig, Jakob; Krahmer, Felix; Kutyniok, Gitta

    2014-01-01

    yield alternative reconstruction procedures. The novel paradigm of sparsity has recently entered the area of frame theory in various ways. Of those different sparsity perspectives, we will focus on the situations where frames and (not necessarily canonical) dual frames can be written as sparse matrices...

  8. An Examination of Test-Retest, Alternate Form Reliability, and Generalizability Theory Study of the easyCBM Reading Assessments: Grade 1. Technical Report #1216

    Science.gov (United States)

    Anderson, Daniel; Park, Jasmine, Bitnara; Lai, Cheng-Fei; Alonzo, Julie; Tindal, Gerald

    2012-01-01

    This technical report is one in a series of five describing the reliability (test/retest/and alternate form) and G-Theory/D-Study research on the easy CBM reading measures, grades 1-5. Data were gathered in the spring 2011 from a convenience sample of students nested within classrooms at a medium-sized school district in the Pacific Northwest. Due…

  9. Proliferation Response to Interleukin-2 and Jak/Stat Activation of T Cells Immortalized by Human T-Cell Lymphotropic Virus Type 1 Is Independent of Open Reading Frame I Expression

    OpenAIRE

    Collins, Nathaniel D.; D’Souza, Celine; Albrecht, Björn; Robek, Michael D.; Ratner, Lee; Ding, Wei; Green, Patrick L.; Lairmore, Michael D.

    1999-01-01

    Human T-cell lymphotropic virus type 1 (HTLV-1), a complex retrovirus, encodes a hydrophobic 12-kD protein from pX open reading frame (ORF) I that localizes to cellular endomembranes and contains four minimal SH3 binding motifs (PXXP). We have demonstrated the importance of ORF I expression in the establishment of infection and hypothesize that p12I has a role in T-cell activation. In this study, we tested interleukin-2 (IL-2) receptor expression, IL-2-mediated proliferation, and Jak/Stat act...

  10. Open reading frame 5 (ORF5), encoding a ferredoxinlike protein, and nifQ are cotranscribed with nifE, nifN, nifX, and ORF4 in Rhodobacter capsulatus.

    OpenAIRE

    Moreno-Vivian, C; Hennecke, S; Pühler, A; Klipp, W

    1989-01-01

    DNA sequence analysis of a 1,600-base-pair fragment located downstream of nifENX in nif region A of Rhodobacter capsulatus revealed two additional open reading frames (ORFs): ORF5, encoding a ferredoxinlike protein, and nifQ. The ferredoxinlike gene product contained two cysteine motifs, typical of ferredoxins coordinating two 4Fe-4S clusters, but the distance between these two motifs was unusual for low-molecular-weight ferredoxins. The R. capsulatus nifQ gene product shared a high degree of...

  11. Complete Nucleotide Sequence and Genome Organization of Hibiscus Chlorotic Ringspot Virus, a New Member of the Genus Carmovirus: Evidence for the Presence and Expression of Two Novel Open Reading Frames

    OpenAIRE

    Huang, Mei; Koh, Dora Chin-Yen; Weng, Li-Juan; Chang, Min-Li; Yap, Yun-Kiam; Zhang, Lee; Wong, Sek-Man

    2000-01-01

    The complete nucleotide sequence of hibiscus chlorotic ringspot virus (HCRSV) was determined. The genomic RNA (gRNA) is 3,911 nucleotides long and has the potential to encode seven viral proteins in the order of 28 (p28), 23 (p23), 81 (p81), 8 (p8), 9 (p9), 38 (p38), and 25 (p25) kDa. Excluding two unique open reading frames (ORFs) encoding p23 and p25, the ORFs encode proteins with high amino acid similarity to those of carmoviruses. In addition to gRNA, two 3′-coterminated subgenomic RNA (s...

  12. Diagnosis and Treatment of Reading Disabilities Based on the Component Model of Reading: An Alternative to the Discrepancy Model of LD

    Science.gov (United States)

    Aaron, P. G.; Joshi, R. Malatesha; Gooden, Regina; Bentum, Kwesi E.

    2008-01-01

    Currently, learning disabilities (LD) are diagnosed on the basis of the discrepancy between students' IQ and reading achievement scores. Students diagnosed with LD often receive remedial instruction in resource rooms. The available evidence suggests that the educational policy based on this discrepancy model has not yielded satisfactory results.…

  13. Approximately dual frame pairs in Hilbert spaces and applications to Gabor frames

    OpenAIRE

    Christensen, Ole; Laugesen, Richard S.

    2008-01-01

    We discuss the concepts of pseudo-dual frames and approximately dual frames, and illuminate their relationship to classical frames. Approximately dual frames are easier to construct than the classical dual frames, and might be tailored to yield almost perfect reconstruction. For approximately dual frames constructed via perturbation theory, we provide a bound on the deviation from perfect reconstruction. An alternative bound is derived for the rich class of Gabor frames, by using the Walnut r...

  14. An Examination of Test-Retest, Alternate Form Reliability, and Generalizability Theory Study of the easyCBM Passage Reading Fluency Assessments: Grade 4. Technical Report #1219

    Science.gov (United States)

    Park, Bitnara Jasmine; Anderson, Daniel; Alonzo, Julie; Lai, Cheng-Fei; Tindal, Gerald

    2012-01-01

    This technical report is one in a series of five describing the reliability (test/retest and alternate form) and G-Theory/D-Study research on the easyCBM reading measures, grades 1-5. Data were gathered in the spring of 2011 from a convenience sample of students nested within classrooms at a medium-sized school district in the Pacific Northwest.…

  15. An Examination of Test-Retest, Alternate Form Reliability, and Generalizability Theory Study of the easyCBM Reading Assessments: Grade 5. Technical Report #1220

    Science.gov (United States)

    Lai, Cheng-Fei; Park, Bitnara Jasmine; Anderson, Daniel; Alonzo, Julie; Tindal, Gerald

    2012-01-01

    This technical report is one in a series of five describing the reliability (test/retest and alternate form) and G-Theory/D-Study research on the easyCBM reading measures, grades 1-5. Data were gathered in the spring of 2011 from a convenience sample of students nested within classrooms at a medium-sized school district in the Pacific Northwest.…

  16. An Examination of Test-Retest, Alternate Form Reliability, and Generalizability Theory Study of the easyCBM Reading Assessments: Grade 2. Technical Report #1217

    Science.gov (United States)

    Anderson, Daniel; Lai, Cheg-Fei; Park, Bitnara Jasmine; Alonzo, Julie; Tindal, Gerald

    2012-01-01

    This technical report is one in a series of five describing the reliability (test/retest an alternate form) and G-Theory/D-Study on the easyCBM reading measures, grades 1-5. Data were gathered in the spring of 2011 from the convenience sample of students nested within classrooms at a medium-sized school district in the Pacific Northwest. Due to…

  17. Fusion Frames and -Frames in Banach Spaces

    Indian Academy of Sciences (India)

    Amir Khosravi; Behrooz Khosravi

    2011-05-01

    Fusion frames and -frames in Hilbert spaces are generalizations of frames, and frames were extended to Banach spaces. In this article we introduce fusion frames, -frames, Banach -frames in Banach spaces and we show that they share many useful properties with their corresponding notions in Hilbert spaces. We also show that -frames, fusion frames and Banach -frames are stable under small perturbations and invertible operators.

  18. Competitive Framing

    OpenAIRE

    Ran Spiegler

    2014-01-01

    I present a simple framework for modeling two-firm market competition when consumer choice is "frame-dependent", and firms use costless "marketing messages" to influence the consumer's frame. This framework embeds several recent models in the "behavioral industrial organization" literature. I identify a property that consumer choice may satisfy, which extends the concept of Weighted Regularity due to Piccione and Spiegler (2012), and provide a characterization of Nash equilibria under this pr...

  19. Physiological Expression and Accumulation of the Products of Two Upstream Open Reading Frames mrtl and MycHex1 Along With p64 and p67 Myc From the Human c-myc Locus.

    Science.gov (United States)

    Ji, Mi Hong; Kim, Seung-Ki; Kim, Chae-Yong; Phi, Ji Hoon; Jun, Hyun Jin; Blume, Scott W; Choi, Hyoung Soo

    2016-06-01

    In addition to the canonical c-Myc p64 and p67 proteins, the human c-myc locus encodes two distinct proteins, mrtl (myc-related translation/localization regulatory factor) and MycHex1 (Myc Human Exon 1), from the upstream open reading frames within the 5'-untranslated region of the c-myc P0 mRNA. The aim of this study is to examine simultaneously, for the first time, mrtl, MycHex1, c-Myc p64, and p67 in human tumor cell lines and pediatric brain tumor tissues. Western blot analysis demonstrated endogenous mrtl, MycHex1, c-Myc p64, and p67 simultaneously. The relative abundance of mrtl and MycHex1 were consistent among a variety of human tumor cell lines, and the relative intensities of mrtl and MycHex1 correlated positively. Confocal imaging revealed mrtl predominantly localized to the nuclear envelope, along with prominent reticular pattern in the cytoplasm. MycHex1 was observed as a series of bright foci located within the nucleus, a subset of which colocalized with fibrillarin. mrtl and MycHex1 co-immunoprecipitated with RACK1, c-Myc, fibrillarin, coilin, and with each other. These findings suggest that mrtl and MycHex1 have multiple interaction partners in both the nucleus and cytoplasm. Sequence analyses confirmed a known polymorphism of mrtl at base 1965 (G>T) and new mutations at bases 1900 (C>G) and 1798 (C>G). Evidence is presented for expression and stable accumulation of all four proteins encoded by three distinct non-overlapping open reading frames within the human c-myc locus. Additional work is warranted to further elucidate the functional or regulatory roles of these molecules in regulation of c-Myc and in oncogenesis. J. Cell. Biochem. 117: 1407-1418, 2016. © 2015 Wiley Periodicals, Inc. PMID:26552949

  20. Discussion on college students' English Reading Skills —intensive reading and extensive reading

    Institute of Scientific and Technical Information of China (English)

    敖登

    2016-01-01

    Students are exposed to a growing amount of vocabularies and knowledge as they step into higher grades, while seem to have little improvement in their reading ability and no alternative means to realize their reading goals. Improving the English reading skills is very important for students as reading enjoys a huge ratio in an English exam paper. Talking about reading, we can not ignore these facts: reading materials, reading speed and reading skills. The followings analyzed are some common skills applied to reading-intensive reading and extensive reading as well as the obstacles that may be met while reading.

  1. Framing Architecture

    OpenAIRE

    Shirey, David E.

    2002-01-01

    The following thesis is comprised of a pair of projects focused on the making of architecture through the concept of framing. The work was conducted at the Academia dà ­Architettura in Mendrisio, Switzerland, under the direction of Peter Zumthor, Miguel Kreisler and Myriam Sterling, emphasizing the relationship between ordered structures and contextual propriety. The making of architecture is inherently most pure when approached as the subtraction of unnecessary elements and a distillation ...

  2. Analysis of Enzymatic Digestion Pattern of Two Open Reading Frames of Varciella–Zoster Genome from Kuwaiti Patients Using the RFLP Technique

    Directory of Open Access Journals (Sweden)

    Jafar A Qasem

    2012-12-01

    Full Text Available Background and Objectives: Varicella–Zoster virus (VZV is a human herpes virus that usually attacks young children and commonly causes chicken pox (Varicella. Following primary infection, a lifelong latent infection is established. The virus often reactivates during adulthood or senesces to cause shingles (Zoster. Little is known regarding the genotypes of Varicella in Kuwait. The aim of this study was to genotype Varicella samples collected from patients in Kuwait.Materials and Methods: Samples from 60 cases of chicken pox were typed. The DNA extraction was performed using the commercially available DNA extraction kit. Two sets of oligonucleotide primers were used to amplify the intervening sequences with polymerase chain reaction to identify VZV DNA in clinical samples. The BglI and PstI endonucleases were used to digest. The PCR amplicons for PCR-RFLP typing.Results: Relatively consistent restriction enzyme digestion profiles for different VZV strains were observed. Limited genetic differences between VZV samples were found. Three VZV strains were identified (A, B and C with type B representing 86.6%, type A 11.7% and type C being 1.7%. We found that distinct restriction fragment length polymorphism isolates from the same origin or nationality were very similar.Conclusion: Varicella strains with cutting sites for both enzyme PstI and BglI (typeB were more prevalent. Molecular amplification of viral DNA by PCR and restriction digestion could be used for VZV typing as an alternative method to serological assays.

  3. Physics of Non-Inertial Reference Frames

    OpenAIRE

    Kamalov, Timur F.

    2007-01-01

    Physics of non-inertial reference frames is a generalizing of Newton's laws to any reference frames. The first, Law of Kinematic in non-inertial reference frames reads: the kinematic state of a body free of forces conserves and determinates a constant n-th order derivative with respect to time being equal in absolute value to an invariant of the observer's reference frame. The second, Law of Dynamic extended Newton's second law to non-inertial reference frames and also contains additional var...

  4. An intron in the thymidylate synthase gene of Bacillus bacteriophage beta 22: evidence for independent evolution of a gene, its group I intron, and the intron open reading frame.

    Science.gov (United States)

    Bechhofer, D H; Hue, K K; Shub, D A

    1994-11-22

    The thymidylate synthase gene (thy) (EC 2.1.1.45) of Bacillus subtilis bacteriophage beta 22 has a self-splicing, group I intron inserted into a highly conserved region of the coding sequence. The intron is very similar to one that is inserted 21 bp further downstream in the homologous thymidylate synthase gene (td) of Escherichia coli bacteriophage T4. In contrast, the amino acid sequences of the bacteriophage thymidylate synthases are highly divergent. The beta 22 intron has a fragmentary open reading frame (ORF) that encodes a putative helix-turn-helix DNA-binding motif, similar to one at the carboxyl terminus of the homing endonuclease (I-TevI) encoded by the T4 td intron. The td ORF and the thy ORF fragments are inserted into different regions of their respective intron structures. These results suggest that the thymidylate synthase genes, their introns, and their respective intron-ORFs all have separate evolutionary histories and that the acquisition of the intron could not have occurred by a simple homing event. PMID:7972121

  5. Open reading frame 5 (ORF5), encoding a ferredoxinlike protein, and nifQ are cotranscribed with nifE, nifN, nifX, and ORF4 in Rhodobacter capsulatus.

    Science.gov (United States)

    Moreno-Vivian, C; Hennecke, S; Pühler, A; Klipp, W

    1989-05-01

    DNA sequence analysis of a 1,600-base-pair fragment located downstream of nifENX in nif region A of Rhodobacter capsulatus revealed two additional open reading frames (ORFs): ORF5, encoding a ferredoxinlike protein, and nifQ. The ferredoxinlike gene product contained two cysteine motifs, typical of ferredoxins coordinating two 4Fe-4S clusters, but the distance between these two motifs was unusual for low-molecular-weight ferredoxins. The R. capsulatus nifQ gene product shared a high degree of homology with Klebsiella pneumoniae and Azotobacter vinelandii NifQ, including a typical cysteine motif located in the C-terminal part. nifQ insertion mutants and also an ORF5-nifQ double deletion mutant showed normal diazotrophic growth only in the presence of high concentrations of molybdate. This demonstrated that the gene encoding the ferredoxinlike protein is not essential for nitrogen fixation. No NifA-activated consensus promoter could be found in the intergenic region between nifENX-ORF4 and ORF5-nifQ. Analyses of a nifQ-lacZYA fusion revealed that transcription of nifQ was initiated at a promoter in front of nifE. In contrast to other nitrogen-fixing organisms, R. capsulatus nifE, nifN, nifX, ORF4, ORF5, and nifQ were organized in one transcriptional unit. PMID:2708314

  6. Immunization with DNA vaccines containing porcine reproductive and respiratory syndrome virus open reading frames 5, 6, and 7 may be related to the exacerbation of clinical disease after an experimental challenge.

    Science.gov (United States)

    Díaz, Ivan; Ganges, Llilianne; Galindo-Cardiel, Iván; Tarradas, Joan; Alvarez, Belén; Lorca-Oró, Cristina; Pujols, Joan; Gimeno, Mariona; Darwich, Laila; Domingo, Mariano; Domínguez, Javier; Mateu, Enric

    2013-02-01

    Pigs were immunized with DNA plasmids containing different open reading frames (ORFs) of a porcine reproductive and respiratory syndrome virus (PRRSV) genotype I strain. One group was injected with three inoculations of ORF7, a second group was immunized with three inoculations of plasmids containing ORF5 and ORF6, and a third group was kept as controls. Later, +21 days after the last inoculation, animals were challenged with the homologous strain. After the challenge, PRRSV-specific interferon (IFN)-γ-secreting cells and anti-PRRSV IgG antibodies developed faster in DNA vaccinated pigs (p<0.05). However, DNA-immunized pigs showed an exacerbation of the disease compared to the unvaccinated challenged pigs. The data suggest that previous immunization with DNA vaccines against glycoprotein 5 and/or matrix protein of PRRSV, as well as nucleoprotein but to a lesser degree, could result in an exacerbation of the clinical course in terms of fever upon challenge. PMID:23409932

  7. Viral culture and p24 antigenemia of human immunodeficiency virus (HIV)-infected individuals correlated with antibody profiles determined with recombinant polypeptides of all HIV-1 open-reading frames.

    Science.gov (United States)

    Baur, A; Vornhagen, R; Korn, K; Sonneborn, H H; Eberlein, B; Harrer, T; Brockhaus, W; Jahn, G

    1992-03-01

    The association between viral activity and antibody profiles was investigated in 202 individuals infected by the human immunodeficiency virus (HIV) grouped according to their Walter Reed clinical stage. Each study group was subdivided into subjects positive or negative for markers of active viral replication: presence of serum p24 antigen and viral culture. In Western blots using recombinant antigens, sera of HIV-positive individuals with positive viral markers had a significantly lower antibody reactivity to several viral proteins than did individuals without viral markers. Noticeably, proteins of the gag (p24, p17) and env (gp120, COOH-terminal part of gp41) open-reading frames revealed a decreased reactivity. The antibody response to the regulatory proteins revealed no or poor association with viral activity in the host. The results suggest that seroreactivity is mainly influenced by factors reflecting the viral activity of an HIV-infected individual, while the clinical stage of the patient is less important. Especially, reductions in antibodies against gp120 and p17 were useful markers associated with increased viral activity. PMID:1371534

  8. Magneto-piezoresistivity in iron particle-filled silicone: An alternative outlook for reading magnetic field intensity and direction

    Directory of Open Access Journals (Sweden)

    V. Iannotti

    2016-01-01

    Full Text Available Elastomagnetic effect (strain induced by magnetic field application and piezoresistivity (change of electron conductivity due to an induced strain are coupled in composite materials constituted by magnetic and conductive microparticles into an elastic matrix. On the basis of these effects, the principle of a new method to read magnetization direction changes, in a random sequence, is proposed and experimentally demonstrated. We have produced new composite magnetopiezoresistive samples, constituted of thin chip shaped Fe microparticles inside a silicone matrix, which under an applied magnetic field along their longitudinal axis, undergo an induced strain depending on the local magnetization direction. The resulting resistivity change can be easily detected and used to deduce the local magnetization direction. The magnetization and strain processes are reversible so that after the removal of external magnetizing field the sample is ready for new measurements. A demonstrator prototype has been conceived, produced and tested. The experimental results provide interesting data encouraging to continue the research towards nano-scale devices in order to pursue the intriguing perspective to achieve a magnetic field gradient sensitivity able to reveal magnetization of semipermanent nanomagnets, polarized ‘up’ and ‘down’.

  9. A 4.6 kb DNA region of Rhizobium meliloti involved in determining urease and hydrogenase activities carries the structural genes for urease (ureA, ureB, ureC) interrupted by other open reading frames.

    Science.gov (United States)

    Miksch, G; Arnold, W; Lentzsch, P; Priefer, U B; Pühler, A

    1994-03-01

    A 4.6 kb DNA region of the Rhizobium meliloti strain AK631 was found to contain seven open reading frames (ORFs), all oriented in the same direction. The putative gene products of four of these ORFs were highly homologous to UreA, UreB and UreC of Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris and Canavalia ensiformis. The overall organisation of the DNA region analysed was ORF1, ureA (ORF2), ORF3, ureB (ORF4), ORF5, ORF6 and ureC (ORF7), indicating that the organisation of the urease structural genes in R. meliloti differs from that of other urease genes so far characterized. ORF1 was incomplete; only the 3' end of the coding region was present. The six complete ORFs coded for polypeptides of 11.1 (UreA), 8.9 (ORF3), 10.8 (UreB), 15.0 (ORF5), 13.8 (ORF6) and 60.7 kDa (UreC). No sequence homology to known polypeptides could be detected for the gene products of ORF1, ORF3, ORF5 and ORF6. Using a lacZ fusion and insertional mutagenesis it was shown that the seven ORFs identified were all located in the same transcription unit. For mutational analysis a resistance gene cassette was introduced into each of the complete ORFs resulting in apolar mutations. Mutations in ureA, ureB and ureC, but not in ORF3, ORF5 and ORF6, abolished urease activity in R. meliloti. The determination of hydrogen uptake in these R. meliloti mutants revealed that only ORF6 and ureB are necessary for hydrogen uptake. PMID:8121412

  10. On frame multiresolution analysis

    DEFF Research Database (Denmark)

    Christensen, Ole

    We use the freedom in frame multiresolution analysis to construct tight wavelet frames (even in the case where the refinable function does not generate a tight frame). In cases where a frame multiresolution does not lead to a construction of a wavelet frame we show how one can nevertheless...... construct a wavelet frame with two generators....

  11. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.

    Directory of Open Access Journals (Sweden)

    Jian Xu

    Full Text Available Hepatitis E virus (HEV is one of the primary causative agents of acute hepatitis, and represents a major cause of severe public health problems in developing countries. The pathogenesis of HEV is not well characterized, however, primarily due to the lack of well-defined cell and animal models. Here, we investigated the effects of genotype 1 HEV open reading frame 3 (ORF3 on TNF-α-induced nucleus factor-κappa B (NF-κB signaling. Human lung epithelial cells (A549 were transiently transfected with ORF3 containing plasmids. These cells were then stimulated with TNF-α and the nucleus translocation of the p65 NF-κB subunit was assessed using western blot and laser confocal microscopy. DNA-binding activity of p65 was also examined using electrophoretic mobility shift assay (EMSA, and the suppression of NF-κB target genes were detected using real-time RT-PCR and ELISA. These results enabled us to identify the decreased phosphorylation levels of IKBα. We focused on the gene of negative regulation of NF-κB, represented by TNF-α-induced protein 3 (TNFAIP3, also known as A20. Reducing the levels of A20 with siRNAs significantly enhances luciferase activation of NF-κB. Furthermore, HEV ORF3 regulated A20 primarily via activating transcription factor 6 (ATF6, involved in unfolded protein response (UPR, resulting in the degradation or inactivation of the receptor interacting protein 1 (RIP1, a major upstream activator of IKB kinase compounds (IKKs. Consequently, the phosphorylation of IKBα and the nucleus translocation of p65 are blocked, which contributes to diminished NF-κB DNA-binding activation and NF-κB-dependent gene expression. The findings suggest that genotype 1 HEV, through ORF3, may transiently activate NF-κB through UPR in early stage, and subsequently inhibit TNF-α-induced NF-κB signaling in late phase so as to create a favorable virus replication environment.

  12. Teaching Reading

    Science.gov (United States)

    Day, Richard R.

    2013-01-01

    "Teaching Reading" uncovers the interactive processes that happen when people learn to read and translates them into a comprehensive easy-to-follow guide on how to teach reading. Richard Day's revelations on the nature of reading, reading strategies, reading fluency, reading comprehension, and reading objectives make fascinating…

  13. On Almost Orthogonal Frames

    OpenAIRE

    Virender; Zothansanga, A.; S. K. Kaushik

    2012-01-01

    Almost orthogonal frames have been introduced and studied. It has been proved that a bounded almost orthogonal frame satisfies Feichtinger conjecture. Also, we prove that a bounded almost orthogonal frame contains a Riesz basis.

  14. On excesses of frames

    OpenAIRE

    Bakić, Damir; Berić, Tomislav

    2014-01-01

    We show that any two frames in a separable Hilbert space that are dual to each other have the same excess. Some new relations for the analysis resp. synthesis operators of dual frames are also derived. We then prove that pseudo-dual frames and, in particular, approximately dual frames have the same excess. We also discuss various results on frames in which excesses of frames play an important role.

  15. About Reading

    Institute of Scientific and Technical Information of China (English)

    独行墨客

    2004-01-01

    As for reading and for learning, reading rate (that is, words per minute, WPM) is important, especially for students who have to pass some reading test. How to compute your reading rate? You may know it after reading the following. Reading Rate (WPM) = Total number of words + reading time.

  16. Adjoint of Pair Frames

    OpenAIRE

    Fereydooni, Abolhassan; Safapour, Ahmad; Rahimi , Asghar

    2012-01-01

    The concept of (p,q)-pair frames is generalized to (l,l^*)-pair frames. Adjoint (conjugate) of a pair frames for dual space of a Banach space is introduced and some conditions for the existence of adjoint (conjugate) of pair frames are presented.

  17. Production of Antibody to Open Reading Frame 3 of Porcine Circovirus Type Ⅱ by DNA Vaccination%DNA免疫法制备猪圆环病毒Ⅱ型ORF3抗体

    Institute of Scientific and Technical Information of China (English)

    谢俊岭; 耿美鸽; 吴迪; 卻小宁; 周双海

    2012-01-01

    The aim of this study was to produce the antibody against the protein of open reading frame 3 (ORF3) of porcine circovirus type II (PCV2), and lay foundation for research on ORF3 function. The ORF3 gene of PCV2 was amplified by PCR method, and the PCR product was digested by restriction enzyme EcoR I and Xho I. Meanwhile, the eukaryotic expression vector pCDNA3.1 (+) was digested by EcoR I and Xho I too. Then, the two expected products digested were ligated, and the transformation in the competent cells of E.coli DHSa was conducted. The results of colony PCR and sequence analysis showed that the recombinant expression vector pCDNA3.1-PCV2 ORF3 was constructed successfully. Thereafter, several BALB/c mice were inoculated with the recombinant plasmid constructed, and the specificity and titer of the antibody to PCV2 ORF3 were detected by immunofluorescence assay (IFA). The IFA results demonstrated that the sera collected from the mice inoculated with the recombinant plasmid PCV2 ORF3 were the antibodies specific to PCV2 ORF3. Also, the IFA results showed that the antibody titer of PCV2 0RF3 in the sera of mice were all more than l:25 after 4 times inoculation, which indicated that the antibodies against PCV2 ORF3 with relatively high titer was successfully produced. The research results revealed that the antibody to PCV2 ORF3 could be produced quickly and simply by vaccination of plasmid DNA, and this provided a new effective way to the production of the antibodies to PCV2 ORF3.%为了制备猪圆环病毒Ⅱ型(PCV2)开放阅读框3(ORF3)蛋白的抗体,并为ORF3功能研究奠定基础,用PCR方法扩增PCV2 ORF3基因,对其PCR产物用EcoRI与XhoI进行酶切,并对真核表达载体pCDNA3.1(+)进行同样酶切,连接2种目的酶切产物后转化E.coliDH5a感受态,茵落PCR和序列测定结果显示成功构建出重组质粒pCDNA3.1-PCV2ORF3.将该重组质粒接种BALB/c小鼠,用间接免疫荧光(IFA)检测小鼠血清PCV2 ORF3抗体的特异性

  18. Relationship between open reading frame 26 subtypes in human herpesvirns-8 and Kaposi's sarcoma%人类疱疹病毒8型ORF26基因亚型与Kaposi肉瘤的相关性研究

    Institute of Scientific and Technical Information of China (English)

    吴秀娟; 普雄明; 吴卫东

    2008-01-01

    Objective To learn the subtypes of open reading frame 26 (ORF26) of human herpesvirus 8 (HHV-8) in patients with Kaposi's sarcoma, and to evaluate the relationship of ORF26 subtypes to clinical types and invasiveness of Kaposi's sarcoma. Methods Thirty-two paraffin-embeded tissue speci-mens of Kaposi's sarcoma were collected in the Department of Dermatology, People's Hospital of Xinjiang Uygur Autonomous Region, from 1996 to 2007. DNA was extracted from these specimens, nested-PCR was used to amplify HHV-8 DNA. PCR products were subjected to bi-directional sequencing after extraction from agarose gels. Phylogenetic analysis was carded out by using the software DNAStar, program Clustal W and PHYLIP package for the determination of ORF26 subtype. Fisher's exact test was performed to evaluate the relationship of ORF26 subtypes to clinical types and invasiveness of Kaposi's sarcoma. Results Of the 32 specimens, 30 were positive for HHV-8 DNA with a positivity rate of 93.75%, and 6 specimens of AIDS related Kaposi's sarcoma were all positive. ORF26 A subtype was detected in the HHV-8 DNA of 17 posi-tive specimens, and C subtype in that of other 13 specimens. Neither the incidence of mucosal lesions nor the distribution of clinical subtypes was of significant difference between patients with ORF26 A subtype and those with ORF26 C subtype (both P > 0.05). Conclusions A and C may be the predominate subtypes of ORF 26 in HHV-8 of patients with Kaposi's sarcoma, and the ORF26 subtype is unrelated to the presence of mucosal lesions in or the clinical types of patients with Kaposi's sarcoma.%目的 明确Kaposi肉瘤患者感染的人类疱疹病毒8型(HHV-8)ORF26基因亚型分类,初步探讨其与Kaposi肉瘤不同临床分型及侵袭性的相关性.方法 对32例Kaposi肉瘤石蜡包埋组织进行HHV-8 DNA抽提、扩增、双向测序,使用DNAStar软件、Clustal W软件和PHYLIP软件包对测序结果进行系统发生学分析,从而确定HHV-8 ORF26基因亚型,

  19. Prime tight frames

    DEFF Research Database (Denmark)

    Lemvig, Jakob; Miller, Christopher; Okoudjou, Kasso A.

    2014-01-01

    to suggest effective analysis and synthesis computation strategies for such frames. Finally, we describe all prime frames constructed from the spectral tetris method, and, as a byproduct, we obtain a characterization of when the spectral tetris construction works for redundancies below two.......We introduce a class of finite tight frames called prime tight frames and prove some of their elementary properties. In particular, we show that any finite tight frame can be written as a union of prime tight frames. We then characterize all prime harmonic tight frames and use thischaracterization...

  20. Optimally Sparse Frames

    CERN Document Server

    Casazza, Peter G; Krahmer, Felix; Kutyniok, Gitta

    2010-01-01

    Frames have established themselves as a means to derive redundant, yet stable decompositions of a signal for analysis or transmission, while also promoting sparse expansions. However, when the signal dimension is large, the computation of the frame measurements of a signal typically requires a large number of additions and multiplications, and this makes a frame decomposition intractable in applications with limited computing budget. To address this problem, in this paper, we introduce sparsity of a frame as a new paradigm. In our terminology, a sparse frame is a frame whose elements have a sparse representation in an orthonormal basis, thereby enabling low-complexity frame decompositions. To introduce a precise meaning of optimality, we take the sum of the numbers of vectors needed of this orthonormal basis when expanding each frame vector as sparsity measure. We then analyze the recently introduced algorithm Spectral Tetris for construction of unit norm tight frames and prove that the tight frames generated...

  1. Bochner PG-Frames

    OpenAIRE

    Rahmani, Morteza; Faroughi, Mohammad H.

    2012-01-01

    In this paper we introduce the concept of Bochner pg-frames for Banach spaces. We characterize the Bochner pg-frames and specify the optimal bounds of a Bochner pg- frames. Then we define a Bochner qg-Riesz basis and verify the relations between Bochner pg-frames and Bochner qg-Riesz bases. Finally, we discuss the perturbation of Bochner pg-frames.

  2. Changing quantum reference frames

    OpenAIRE

    Palmer, Matthew C; Girelli, Florian; Bartlett, Stephen D.

    2013-01-01

    We consider the process of changing reference frames in the case where the reference frames are quantum systems. We find that, as part of this process, decoherence is necessarily induced on any quantum system described relative to these frames. We explore this process with examples involving reference frames for phase and orientation. Quantifying the effect of changing quantum reference frames serves as a first step in developing a relativity principle for theories in which all objects includ...

  3. Nucleotide sequence and mutational analysis of the structural genes (anfHDGK) for the second alternative nitrogenase from Azotobacter vinelandii.

    OpenAIRE

    Joerger, R D; Jacobson, M R; Premakumar, R; Wolfinger, E D; Bishop, P E

    1989-01-01

    The nucleotide sequence of a region of the Azotobacter vinelandii genome exhibiting sequence similarity to nifH has been determined. The order of open reading frames within this 6.1-kilobase-pair region was found to be anfH (alternative nitrogen fixation, nifH-like gene), anfD (nifD-like gene), anfG (potentially encoding a protein similar to the product of vnfG from Azotobacter chroococcum), anfK (nifK-like gene), followed by two additional open reading frames. The 5'-flanking region of anfH ...

  4. Videotaped Oral Reading Fluency Lab: An Alternative Approach to One-on-One Interventions for Intermediate Elementary Students with Learning Disabilities

    Science.gov (United States)

    Christner, Beth Anne Reside

    2009-01-01

    The ability to read aloud fluently is a reflection of one's ability to automatically decode words and comprehend text at the same time (Samuels, 2006), a task which may be difficult for many intermediate elementary students with learning disabilities (LD) (Ferrara, 2005). Previous research shows that audio-assisted repeated readings and…

  5. An Examination of Test-Retest, Alternate Form Reliability, and Generalizability Theory Study of the easyCBM Word and Passage Reading Fluency Assessments: Grade 3. Technical Report #1218

    Science.gov (United States)

    Park, Bitnara Jasmine; Anderson, Daniel; Alonzo, Julie; Lai, Cheng-Fei; Tindal, Gerald

    2012-01-01

    This technical report is one in a series of five describing the reliability (test/retest and alternate form) and G-Theory/D-Study research on the easyCBM reading measures, grades 1-5. Data were gathered in the spring of 2011 from a convenience sample of students nested within classrooms at a medium-sized school district in the Pacific Northwest.…

  6. Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.

    Science.gov (United States)

    Yang, Xinping; Coulombe-Huntington, Jasmin; Kang, Shuli; Sheynkman, Gloria M; Hao, Tong; Richardson, Aaron; Sun, Song; Yang, Fan; Shen, Yun A; Murray, Ryan R; Spirohn, Kerstin; Begg, Bridget E; Duran-Frigola, Miquel; MacWilliams, Andrew; Pevzner, Samuel J; Zhong, Quan; Trigg, Shelly A; Tam, Stanley; Ghamsari, Lila; Sahni, Nidhi; Yi, Song; Rodriguez, Maria D; Balcha, Dawit; Tan, Guihong; Costanzo, Michael; Andrews, Brenda; Boone, Charles; Zhou, Xianghong J; Salehi-Ashtiani, Kourosh; Charloteaux, Benoit; Chen, Alyce A; Calderwood, Michael A; Aloy, Patrick; Roth, Frederick P; Hill, David E; Iakoucheva, Lilia M; Xia, Yu; Vidal, Marc

    2016-02-11

    While alternative splicing is known to diversify the functional characteristics of some genes, the extent to which protein isoforms globally contribute to functional complexity on a proteomic scale remains unknown. To address this systematically, we cloned full-length open reading frames of alternatively spliced transcripts for a large number of human genes and used protein-protein interaction profiling to functionally compare hundreds of protein isoform pairs. The majority of isoform pairs share less than 50% of their interactions. In the global context of interactome network maps, alternative isoforms tend to behave like distinct proteins rather than minor variants of each other. Interaction partners specific to alternative isoforms tend to be expressed in a highly tissue-specific manner and belong to distinct functional modules. Our strategy, applicable to other functional characteristics, reveals a widespread expansion of protein interaction capabilities through alternative splicing and suggests that many alternative "isoforms" are functionally divergent (i.e., "functional alloforms"). PMID:26871637

  7. Physics of Non-Inertial Reference Frames

    International Nuclear Information System (INIS)

    Physics of non-inertial reference frames is a generalizing of Newton's laws to any reference frames. It is the system of general axioms for classical and quantum mechanics. The first, Kinematics Principle reads: the kinematic state of a body free of forces conserves and equal in absolute value to an invariant of the observer's reference frame. The second, Dynamics Principle extended Newton's second law to non-inertial reference frames and also contains additional variables there are higher derivatives of coordinates. Dynamics Principle reads: a force induces a change in the kinematic state of the body and is proportional to the rate of its change. It is mean that if the kinematic invariant of the reference frame is n-th derivative with respect the time, then the dynamics of a body being affected by the force F is described by the 2n-th differential equation. The third, Statics Principle reads: the sum of all forces acting a body at rest is equal to zero.

  8. Performance Comparison of Binary Search Tree and Framed ALOHA Algorithms for RFID Anti-Collision

    Science.gov (United States)

    Chen, Wen-Tzu

    Binary search tree and framed ALOHA algorithms are commonly adopted to solve the anti-collision problem in RFID systems. In this letter, the read efficiency of these two anti-collision algorithms is compared through computer simulations. Simulation results indicate the framed ALOHA algorithm requires less total read time than the binary search tree algorithm. The initial frame length strongly affects the uplink throughput for the framed ALOHA algorithm.

  9. Human cytomegalovirus UL144 open reading frame: sequence variability in Guangzhou congenital infected children%广州地区HCMV临床病毒株UL144 ORF的序列变异研究

    Institute of Scientific and Technical Information of China (English)

    王波; 李月琴; 叶宁; 胡兢晶; 何震宇; 田传军; 张纯青; 叶铁真; 周天鸿

    2008-01-01

    目的 研究广州地区先天性感染的人巨细胞病毒(HCMV)临床低传代分离病毒株UL144基因序列的多态性,探讨UL144基因在HCMV致病中的作用.方法 对3株经多重PCR鉴定HCMV DNA为阳性的临床低传代分离株进行HCMV UL144基因全序列PCR扩增,PCR产物克隆到pMD18-T载体上再测序,将其序列与GenBank中公布的其它10株临床分离株UL144基因一起进行分析.结果 本实验克隆并测序了HCMV临床低传代D3、D2和D52病毒株的UL144基因,提交GenBank,已被GenBank收录,序列号分别为DQ180368、DQ180382和DQ180355.HCMV临床低传代D3、D2和D52病毒株的UL144基因均全长531 bp.通过blast分析,从GenBank中找到了10株HCMV病毒株的UL144与D3、D2和D52的UL144基因具有较高的同源性,经过序列的比对,发现UL144基因DNA序列比较保守,只在4处有变异,且变异均为碱基替换,无插入或缺失,编码蛋白由176个氨基酸残基组成,氨基酸序列也比较保守,各分离株中变异率为1.1%;HCMV UL144编码蛋白翻译后修饰位点在所有分离株中均高度保守;所有分离株UL144蛋白的等电点均为8.97.结论 广州地区临床低传代分离株HCMV UL144基因DNA及其编码产物的氨基酸序列是比较保守的,但仍存在一定的多态性.提示UL144基因在先天性感染中可能具有重要作用.%Objective To investigate the polymorphism of human cytomegalovirus (HCMV) UL144 gene of the low passage clinical isolates in Guangzhou and explore the role of UL144 gene in HCMV pathogenicity. Methods The clinical isolates of HCMV were obtained from the urine sample collected from those infants with intra-uterus HCMV infection in Guangzhou. The virus genome DNA was extracted. According to the genome sequence of Toledo, primers for UL144 gene were designed and used to amplify the complete open reading frames (ORF) of the UL144 gene in our 3 different clinical isolates. These ORFs of the UL144 gene were cloned into pMD18-T vector

  10. Filter Bank Fusion frames

    OpenAIRE

    Chebira, Amina; Fickus, Matthew; Mixon, Dustin G.

    2011-01-01

    In this paper we characterize and construct novel oversampled filter banks implementing fusion frames. A fusion frame is a sequence of orthogonal projection operators whose sum can be inverted in a numerically stable way. When properly designed, fusion frames can provide redundant encodings of signals which are optimally robust against certain types of noise and erasures. However, up to this point, few implementable constructions of such frames were known; we show how to construct them using ...

  11. Grassmannian Fusion Frames

    OpenAIRE

    King, Emily J.

    2010-01-01

    Transmitted data may be corrupted by both noise and data loss. Grassmannian frames are in some sense optimal representations of data transmitted over a noisy channel that may lose some of the transmitted coefficients. Fusion frame (or frame of subspaces) theory is a new area that has potential to be applied to problems in such fields as distributed sensing and parallel processing. Grassmannian fusion frames combine elements from both theories. A simple, novel construction of Grassmannian fusi...

  12. Approximately dual frames in Hilbert spaces and applications to Gabor frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Laugesen, Richard S.

    2011-01-01

    Approximately dual frames are studied in the Hilbert space setting. Approximate duals are easier to construct than classical dual frames, and can be tailored to yield almost perfect reconstruction. Bounds on the deviation from perfect reconstruction are obtained for approximately dual frames...... constructed via perturbation theory. An alternative bound is derived for the rich class of Gabor frames, by using the Walnut representation of the frame operator to estimate the deviation from equality in the duality conditions. To illustrate these results, we construct explicit approximate duals of Gabor...... frames generated by the Gaussian; these approximate duals yield almost perfect reconstruction. Surprisingly, the method applies also to certain Gabor frames that are far from being tight....

  13. Pragmatic Issues Related to Reading Comprehension Questions: A Case Study From a Latino Bilingual Classroom

    OpenAIRE

    Field, Margaret

    1996-01-01

    This paper addresses some of the challenges which bilingual children transitioning to literacy in English may face when asked to answer reading comprehension questions which involve the interpretation and synthesis ofinformation about story characters' thoughts or feelings. Understanding of a character's perspective may depend on inference, rather than lexical content presented in a text Alternatively, prompt-questions may be framed such that lexical story content is required in the answer Su...

  14. Reading faster

    OpenAIRE

    Paul Nation

    2009-01-01

    This article describes the visual nature of the reading process as it relates to reading speed. It points out that there is a physical limit on normal reading speed and beyond this limit the reading process will be different from normal reading where almost every word is attended to. The article describes a range of activities for developing reading fluency, and suggests how the development of fluency can become part of a reading programme.

  15. Dynamics of students' epistemological framing in group problem solving

    CERN Document Server

    Nguyen, Hai D; Sayre, Eleanor C

    2016-01-01

    Many studies have investigated students' epistemological framing when solving physics problems. Framing supports students' problem solving as they decide what knowledge to employ and the necessary steps to solve the problem. Students may frame the same problem differently and take alternate paths to a correct solution. When students work in group settings, they share and discuss their framing to decide how to proceed in problem solving as a whole group. In this study, we investigate how groups of students negotiate their framing and frame shifts in group problem solving.

  16. On the interaction between media frames and individual frames of the Israeli-Palestinian conflict

    Directory of Open Access Journals (Sweden)

    Wilhelm Kempf

    2012-10-01

    Full Text Available The present paper reports on a media effects research experiment in which six groups of participants were asked to read and evaluate differently framed news articles about two scenarios: a Palestinian attack on Israel and an Israeli military operation against Palestinians. The experimental results show that media peace frames of violent events in the Israeli-Palestinian conflict are generally regarded by the German public as more comprehensible, less biased, more balanced and less partisan than media war frames of the same events. The specific ways in which recipients respond to the frames, however, depend on their prior knowledge of the conflict, on their positioning to the conflict and on their sensitivity to the ambivalence of war and peace for both Israel and the Palestinians. This supports the hypothesis that neither news selection nor framing have uniform effects on public opinion.

  17. Changing climate, changing frames

    International Nuclear Information System (INIS)

    Highlights: ► We show development of flood policy frames in context of climate change attention. ► Rising attention on climate change influences traditional flood policy framing. ► The new framing employs global-scale scientific climate change knowledge. ► With declining attention, framing disregards climate change, using local knowledge. ► We conclude that frames function as sensemaking devices selectively using knowledge. -- Abstract: Water management and particularly flood defence have a long history of collective action in low-lying countries like the Netherlands. The uncertain but potentially severe impacts of the recent climate change issue (e.g. sea level rise, extreme river discharges, salinisation) amplify the wicked and controversial character of flood safety policy issues. Policy proposals in this area generally involve drastic infrastructural works and long-term investments. They face the difficult challenge of framing problems and solutions in a publicly acceptable manner in ever changing circumstances. In this paper, we analyse and compare (1) how three key policy proposals publicly frame the flood safety issue, (2) the knowledge referred to in the framing and (3) how these frames are rhetorically connected or disconnected as statements in a long-term conversation. We find that (1) framings of policy proposals differ in the way they depict the importance of climate change, the relevant timeframe and the appropriate governance mode; (2) knowledge is selectively mobilised to underpin the different frames and (3) the frames about these proposals position themselves against the background of the previous proposals through rhetorical connections and disconnections. Finally, we discuss how this analysis hints at the importance of processes of powering and puzzling that lead to particular framings towards the public at different historical junctures

  18. Filter Bank Fusion Frames

    CERN Document Server

    Chebira, Amina; Mixon, Dustin G

    2010-01-01

    In this paper we characterize and construct novel oversampled filter banks implementing fusion frames. A fusion frame is a sequence of orthogonal projection operators whose sum can be inverted in a numerically stable way. When properly designed, fusion frames can provide redundant encodings of signals which are optimally robust against certain types of noise and erasures. However, up to this point, few implementable constructions of such frames were known; we show how to construct them using oversampled filter banks. In this work, we first provide polyphase domain characterizations of filter bank fusion frames. We then use these characterizations to construct filter bank fusion frame versions of discrete wavelet and Gabor transforms, emphasizing those specific finite impulse response filters whose frequency responses are well-behaved.

  19. Discretization of Continuous Frame

    Indian Academy of Sciences (India)

    A Fattahi; H Javanshiri

    2012-05-01

    In this paper we consider the notion of continuous frame of subspaces and define a new concept of continuous frame, entitled continuous atomic resolution of identity, for arbitrary Hilbert space $\\mathcal{H}$ which has a countable reconstruction formula. Among the other results, we characterize the relationship between this new concept and other known continuous frames. Finally, we state and prove the assertions of the stability of perturbation in this concept.

  20. Reference frames and refbits

    CERN Document Server

    Van Enk, S J

    2004-01-01

    We define a new quantity called refbit, which allows one to quantify the resource of sharing a reference frame in quantum communication protocols. By considering various protocols we find relations between refbits and other resources such as cbits, ebits, cobits, and qubits. We also consider the same resources in encoded, reference-frame independent, form. This allows one to rephrase and unify previous work on phase references, reference frames, and superselection rules.

  1. Soul of the Documentary. Framing, Expression, Ethics

    OpenAIRE

    Hongisto, Ilona

    2015-01-01

    ‘Soul of the Documentary’ offers a groundbreaking new approach to documentary cinema. Ilona Hongisto stirs current thinking by suggesting that the work of documentary films is not reducible to representing what already exists. By close-reading a diverse body of films - from ‘The Last Bolshevik’ to ‘Grey Gardens’ - Hongisto shows how documentary cinema intervenes in the real by framing it and creatively contributes to its perpetual unfolding. The emphasis on framing brings new urgency to the d...

  2. Framings and dilations

    OpenAIRE

    Larson, David R.; Szafraniec, Franciszek Hugon

    2013-01-01

    The notion of framings, recently emerging in P. G. Casazza, D. Han, and D. R. Larson, Frames for Banach spaces, in {\\em The functional and harmonic analysis of wavelets and frames} (San Antonio, TX, 1999), {\\em Contemp. Math}. {\\bf 247} (1999), 149-182 as generalization of the reconstraction formula generated by pairs of dual frames, is in this note extended substantially. This calls on refining the basic dilation results which still being in the flavor of {\\em th\\'eor\\`eme principal} of B. S...

  3. Associations between reading achievement and independent reading in early elementary school: A genetically-informative cross-lagged study

    OpenAIRE

    Harlaar, Nicole; Deater-Deckard, Kirby; Thompson, Lee A.; DeThorne, Laura S.; Petrill, Stephen A.

    2011-01-01

    This study used a cross-lagged twin design to examine reading achievement and independent reading from 10 to 11 years (n = 436 twin pairs). Reading achievement at age 10 significantly predicted independent reading at age 11. The alternative path, from independent reading at age 10 to reading achievement at age 11, was not significant. Individual differences in reading achievement and independent reading at both ages were primarily due to genetic influences. Furthermore, individual differences...

  4. Irregular wavelet frames

    Institute of Scientific and Technical Information of China (English)

    孙文昌; 周性伟

    2000-01-01

    For the non-band-limited function ψ, a sufficient condition is presented under whichis a frame for L2(R). The stability of these frames is studied. For the wavelets frequently used in signal processing, some concrete results are given.

  5. Fusion Frames: Existence and Construction

    CERN Document Server

    Calderbank, Robert; Heinecke, Andreas; Kutyniok, Gitta; Pezeshki, Ali

    2009-01-01

    Fusion frame theory is an emerging mathematical theory that provides a natural framework for performing hierarchical data processing. A fusion frame is a frame-like collection of subspaces in a Hilbert space, thereby generalizing the concept of a frame for signal representation. In this paper, we study the existence and construction of fusion frames. We first present a complete characterization of a special class of fusion frames, called Parseval fusion frames. The value of Parseval fusion frames is that the inverse fusion frame operator is equal to the identity and therefore signal reconstruction can be performed with minimal complexity. We then introduce two general methods -- the spatial complement and the Naimark complement -- for constructing a new fusion frame from a given fusion frame. We then establish existence conditions for fusion frames with desired properties. In particular, we address the following question: Given $M, N, m \\in \\NN$ and $\\{\\lambda_j\\}_{j=1}^M$, does there exist a fusion frame in ...

  6. Framing (implicitly) matters

    DEFF Research Database (Denmark)

    Anderson, Joel; Antalikova, Radka

    2014-01-01

    Denmark is currently experiencing the highest immigration rate in its modern history. Population surveys indicate that negative public attitudes toward immigrants actually stem from attitudes toward their (perceived) Islamic affiliation. We used a framing paradigm to investigate the explicit and ...

  7. Framing French Culture

    OpenAIRE

    Edwards, Natalie; McCann, Ben; Poiana, Peter

    2015-01-01

    Throughout this book, the concept of framing is used to look at art, photography, scientific drawings and cinema as visually constituted, spatially bounded productions. The way these genres relate to that which exists beyond the frame, by means of plastic, chemically transposed, pencil-sketched or moving images allows us to decipher the particular language of the visual and at the same time circumscribe the dialectic between presence and absence that is proper to all visual media. Yet, these ...

  8. Space-Frame Antenna

    Science.gov (United States)

    Curtis, Steven A.

    2010-01-01

    The space-frame antenna is a conceptual antenna structure that would be lightweight, deployable from compact stowage, and capable of deforming itself to a size, shape, and orientation required for a specific use. The space-frame antenna would be a trusslike structure consisting mostly of a tetrahedral mesh of nodes connected by variable-length struts. The deformation of the antenna to a desired size, shape, and orientation would be effected through coordinated lengthening and shorting of the struts.

  9. On transforms between Gabor frames and wavelet frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Goh, Say Song

    2013-01-01

    We describe a procedure that enables us to construct dual pairs of wavelet frames from certain dual pairs of Gabor frames. Applying the construction to Gabor frames generated by appropriate exponential Bsplines gives wavelet frames generated by functions whose Fourier transforms are compactly...... supported splines with geometrically distributed knot sequences. There is also a reverse transform, which yields pairs of dual Gabor frames when applied to certain wavelet frames....

  10. Nonstationary Gabor Frames - Approximately Dual Frames and Reconstruction Errors

    OpenAIRE

    Dörfler, Monika; Matusiak, Ewa

    2013-01-01

    Nonstationary Gabor frames, recently introduced in adaptive signal analysis, represent a natural generalization of classical Gabor frames by allowing for adaptivity of windows and lattice in either time or frequency. Due to the lack of a complete lattice structure, perfect reconstruction is in general not feasible from coefficients obtained from nonstationary Gabor frames. In this paper it is shown that for nonstationary Gabor frames that are related to some known frames for which dual frames...

  11. Calculating body frame size (image)

    Science.gov (United States)

    Body frame size is determined by a person's wrist circumference in relation to his height. For example, a man ... would fall into the small-boned category. Determining frame size: To determine the body frame size, measure ...

  12. Numerically erasure-robust frames

    OpenAIRE

    Fickus, Matthew; Mixon, Dustin G.

    2012-01-01

    Given a channel with additive noise and adversarial erasures, the task is to design a frame that allows for stable signal reconstruction from transmitted frame coefficients. To meet these specifications, we introduce numerically erasure-robust frames. We first consider a variety of constructions, including random frames, equiangular tight frames and group frames. Later, we show that arbitrarily large erasure rates necessarily induce numerical instability in signal reconstruction. We conclude ...

  13. The structure of an alternate form of complement C3 that displays costimulatory growth factor activity for B lymphocytes

    OpenAIRE

    1994-01-01

    In this study, the structure of a novel 1.9-kb transcript coding for complement component 3 (C3) is described. This alternate C3 is identical to the 3' end of the C3 message beginning at position 3300 of the C3 cDNA. Its transcription appears to be driven by an alternate promoter located within intron 8 of the C3 gene. This alternate C3 message contains an open reading frame that may encode a 536-amino acid- long protein identical to the 3' part of the C3 alpha chain. The resulting protein co...

  14. Found-Footage Horror and the Frame's Undoing

    OpenAIRE

    Sayad, Cecilia

    2016-01-01

    This essay finds in the found-footage horror cycle an alternative way of understanding the relationship between horror films and reality, which is usually discussed in terms of allegory. I propose the investigation of framing, understood both figuratively (framing the film as documentary) and stylistically (the framing in handheld cameras and in static long takes), as a device that playfully de-stabilizes the separation between the film and the surrounding world. The essay’s main case study i...

  15. Design concepts for low-cost composite turbofan engine frame

    Science.gov (United States)

    Mitchell, S. C.; Stoffer, L. J.

    1980-01-01

    Design concepts for low cost, lightweight composite engine frames were applied to the design requirements for the frame of a commercial, high bypass engine. Four alternative composite frame design concepts identified which consisted of generic type components and subcomponents that could be adapted to use in different locations in the engine and the different engine sizes. A variety of materials and manufacturing methods were projected with a goal for the lowest number of parts at the lowest possible cost. After a preliminary evaluation of all four frame concepts, two designs were selected for an extended design and evaluation which narrowed the final selection down to one frame that was significantly lower in cost and slighty lighter than the other frame. An implementation plan for this lowest cost frame is projected for future development and includes prospects for reducing its weight with proposed unproven, innovative fabrication techniques.

  16. What's in a Frame?

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    Maintaining a good image and reputation in the eyes of stakeholders is vital to the organisation. Thus, in its corporate communication and discourse the organisation will seek to present or frame itself as favourably as possible while observing regulations stipulating accuracy and precision in the...... considerable strain on the Bank and its image, leading it to reconsider its previous strategy of denial of responsibility. References Entman, Robert M. 1993. Framing: Toward clarification of a fractured paradigm. Journal of Communication, 41: 6-25. Gunnarsson, Britt-Louise. 2009. Professional Discourse. London...... organisation, and hence in shaping the image projected to the public. Framing is here understood as the selection of ‘some aspects of perceived reality … [making] them more salient in the communication text, in such a way as to promote a particular problem definition, causal interpretation, moral evaluation...

  17. Framing Financial Culture

    DEFF Research Database (Denmark)

    Just, Sine Nørholm; Mouton, Nico

    2014-01-01

    conceptual framework that combines modern framing theory and classical stasis theory is applied to the rhetorical struggles over the meaning of ‘Liborgate’. Findings: While rhetorical struggles over ‘Liborgate’ overtly center on the issue of who is to blame, an analysis of the argumentative relations between...... competing frames leads to the conclusion that this political ‘blame game’ is related to struggles over how to define the scandal, how to conceptualize its causes, and policy recommendations. Banks may have lost the battle of ‘Liborgate’, but the war over the meaning of financial culture is far from over....... Originality/value: The paper is theoretically and methodologically original in its combination of the theories of framing and stasis, and it provides analytical insights into how sense is made of financial culture in the wake of the financial crisis....

  18. New Characterizations of Fusion Frames (Frames of Subspaces)

    Indian Academy of Sciences (India)

    Mohammad Sadegh Asgari

    2009-06-01

    In this article, we give new characterizations of fusion frames, on the properties of their synthesis operators, on the behavior of fusion frames under bounded operators with closed range, and on erasures of subspaces of fusion frames. Furthermore we show that every fusion frame is the image of an orthonormal fusion basis under a bounded surjective operator.

  19. Video frame processor

    International Nuclear Information System (INIS)

    This report provides technical description regarding the Video Frame Processor (VFP) developed at Bhabha Atomic Research Centre. The instrument provides capture of video images available in CCIR format. Two memory planes each with a capacity of 512 x 512 x 8 bit data enable storage of two video image frames. The stored image can be processed on-line and on-line image subtraction can also be carried out for image comparisons. The VFP is a PC Add-on board and is I/O mapped within the host IBM PC/AT compatible computer. (author). 9 refs., 4 figs., 19 photographs

  20. Institutional Justification in Frames

    DEFF Research Database (Denmark)

    Baden, Christian; Schultz, Friederike

    Crises – such as the BP oil spill in the Gulf of Mexico (2010) – are moments in which conventional interpretations break down. This paper analyzes the dynamics of the re-construction and stabilization of meaning in the public debate and assesses how BP’s strategic framing affected the emerging...... constructed frames. In a semantic network analysis based on over 3700 news articles from the US and UK and BP’s press releases, we document continuities and disruptions in re-emerging meaning. The paper shows that frames’ diversified bases of legitimacy are instrumental to securing widespread acceptance and...

  1. Framing the Manager

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    2013-01-01

    predominant conceptual models or frames for leadership, the ‘goal-oriented motivator’ model and the ‘empathic communicator’ model, which frame the position and the candidate, from the discursive and metaphorical construction of the ad to the actual hiring of the new employee. While there may be little new in...... this realisation, the fact that one of the two models, the ‘goal-oriented motivator’ model, seems to be monopolising the genre raises a number of issues that need to be addressed: How is this model realised conceptually and linguistically? Why does this model continue to be the Danish business world...

  2. Alternative Sigma Factor HrpL of Pectobacterium carotovorum 35 is Important for the Development of Soft-rot Symptoms

    OpenAIRE

    Hyo-Song Nam; Ju Yeon Park; Young Cheol Kim; Beom Ryong Kang; Sung-Hee Lee; Jae Soon Cha

    2011-01-01

    A bacterial artificial chromosome library of Pectobacterium carotovorum 35 was constructed to characterize the genome and to sequence its hrp region. The hrp cluster of P. carotovorum 35 consisted of 26 open reading frames in five operons. A promoter-based green fluorescent protein technology was used to identify the genes regulated by the alternative sigma factor, HrpL, in P. carotovorum 35. The majority of the selected clones contained the hrpJ operon promoter sequence, which harbors a hrp ...

  3. A Case for Slow Reading

    Directory of Open Access Journals (Sweden)

    Matthew J Ostercamp

    2014-05-01

    Full Text Available This essay makes a case for the value of slow or deep reading.  Inspired by the Slow Food movement it seeks to apply their principles to reading.  It begins by exploring the meaning of information and how like food, information has come to be regarded as a commodity.  Drawing upon the philosophy of Albert Borgmann, it counters the prevalent commodity view of information by offering an alternative paradigm that connects careful reading to human flourishing.  It argues that by connecting information to pleasure and community, slow reading advocates can have comparable success to that enjoyed by the slow food movement.

  4. Frames for supersymmetry

    CERN Document Server

    Doran, C F; Gates, S J, Jr; Hübsch, T; Iga, K M; Landweber, G D

    2008-01-01

    We explain how the redefinitions of supermultiplet component fields, comprising what we call "frame shifts", can be used in conjuction with the graphical technology of multiplet Adkinras to render manifest the reducibility of off-shell representations of supersymmetry. This technology speaks to possibility of organizing multiplet constraints in a way which complements and extends the possibilities afforded by superspace methods.

  5. Global Vertical Reference Frame

    Czech Academy of Sciences Publication Activity Database

    Burša, Milan; Kenyon, S.; Kouba, J.; Šíma, Zdislav; Vatrt, V.; Vojtíšková, M.

    -, č. 5 (2009), s. 53-63. ISSN 1801-8483 R&D Projects: GA ČR GA205/08/0328 Institutional research plan: CEZ:AV0Z10030501 Keywords : sea surface topography * satellite altimetry * vertical frames Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics

  6. Framing Evolution Discussion Intellectually

    Science.gov (United States)

    Oliveira, Alandeom W.; Cook, Kristin; Buck, Gayle A.

    2011-01-01

    This study examines how a first-year biology teacher facilitates a series of whole-class discussions about evolution during the implementation of a problem-based unit. A communicative theoretical perspective is adopted wherein evolution discussions are viewed as social events that the teacher can frame intellectually (i.e., present or organize as…

  7. The Cage; Framing Dad

    DEFF Research Database (Denmark)

    Kau, Edvin

    2012-01-01

    Unfolding his story very gradually and arousing the viewer’s curiosity, Sitaru invites the audience to investigate the parents’ and the boy’s mutual positions in their small flat, as well as the various layers of their conversations, through such means as framing, editing style, and the use of...

  8. The BRCA1 alternative splicing variant delta14-15 with an in-frame deletion of part of the regulatory serine-containing domain (SCD) impairs the DNA repair capacity in MCF-7 cells

    Czech Academy of Sciences Publication Activity Database

    Ševčík, J.; Falk, Martin; Kleiblová, P.; Lhota, F.; Štefančíková, L.; Janatová, M.; Weiterová, Lenka; Lukášová, Emilie; Kozubek, Stanislav; Pohlreich, P.; Kleibl, Z.

    2012-01-01

    Roč. 24, č. 5 (2012), s. 1023-1030. ISSN 0898-6568 R&D Projects: GA AV ČR(CZ) IAA500040802 Institutional research plan: CEZ:AV0Z50040702 Keywords : BRCA1 * alternative splicing * DNA repair Subject RIV: BO - Biophysics Impact factor: 4.304, year: 2012

  9. Spectral Tetris Fusion Frame Constructions

    CERN Document Server

    Casazza, Peter G; Heinecke, Andreas; Wang, Yang; Zhou, Zhengfang

    2011-01-01

    Spectral tetris is a flexible and elementary method to construct unit norm frames with a given frame operator having all of its eigenvalues greater than or equal to two. One important application of this method is the construction of fusion frames. We provide a sufficient condition for using spectral tetris to construct a fusion frame with prescribed eigenvalues for its fusion frame operator and with prescribed dimensions for its subspaces. This condition is shown to be also necessary in the tight case. We then generalize spectral tetris to construct unit norm tight frames of redundancy less than two and use it to derive non-equidimensional tight fusion frames.

  10. Framing difficulties in quantum mechanics

    CERN Document Server

    Modir, Bahar; Sayre, Eleanor C

    2016-01-01

    Students' difficulties in quantum mechanics may be the result of unproductive framing and not fundamental inability to solve the problems or misconceptions about physics content. We observed groups of students solving quantum mechanics problems in an upper-division physics course. Using the lens of the epistemological framing, we investigated four frames in our observational data: algorithmic math, conceptual math, algorithmic physics, and conceptual physics. We then used our framework to seek an underlying structure to the long lists of published difficulties that span many topics in quantum mechanics. We mapped descriptions of published difficulties into errors in epistemological framing and resource use. We analyzed descriptions of students' problem solving to find their frames, and compared students' framing to framing (and frame shifting) required by problem statements. We found three categories of error: mismatches between students' framing and problem statement framing; inappropriate or absent transiti...

  11. Wavelet frames and their duals

    DEFF Research Database (Denmark)

    Lemvig, Jakob

    2008-01-01

    This thesis is concerned with computational and theoretical aspects of wavelet frame analysis in higher dimensions and, in particular, with the study of so-called dual frames of wavelet frames. A frame is a system of "simple" functions or building blocks which deliver ways of analyzing signals. The...... signals are then represented by linear combinations of the building blocks with coefficients found by an associated frame, called a dual frame. A wavelet frame is a frame where the building blocks are stretched (dilated) and translated versions of a single function; such a frame is said to have wavelet...... structure. The dilation of the wavelet building blocks in higher dimension is done via a square matrix which is usually taken to be integer valued. In this thesis we step away from the "usual" integer, expansive dilation and consider more general, expansive dilations. In most applications of wavelet frames...

  12. Equiangular Frames and Signature Sets

    OpenAIRE

    Singh, Preeti

    2009-01-01

    We will present a relation between real equiangular frames and certain special sets in groups which we call signature sets and show that many equiangular frames arise in this manner. Then we will define quasi-signature sets and will examine equiangular frames associated to these subsets of groups. We will extend these results to complex equiangular frames where the inner product between any pair of vectors is a common multiple of a cube root of unity and exhibit equiangular frames that arise ...

  13. Framing finance: A methodological account

    OpenAIRE

    Sheila C. Dow

    2013-01-01

    The way in which financial markets are framed depends on who is doing the framing, although there are reflexive interdependencies between these framings. Mainstream economics frames financial markets as archetypical competitive markets, focusing on prices as the key information on which to base analysis. This follows from traditional positivist methodology where computability is the key to theory appraisal. Central banks draw on this analysis for their own framing, but modify it significantly...

  14. Tight p-fusion frames

    OpenAIRE

    Bachoc, Christine; Ehler, Martin

    2012-01-01

    Fusion frames enable signal decompositions into weighted linear subspace components. For positive integers p, we introduce p-fusion frames, a sharpening of the notion of fusion frames. Tight p-fusion frames are closely related to the classical notions of designs and cubature formulas in Grassmann spaces and are analyzed with methods from harmonic analysis in the Grassmannians. We define the p-fusion frame potential, derive bounds for its value, and discuss the connections to tight p-fusion fr...

  15. Some relationship between G-frames and frames

    Directory of Open Access Journals (Sweden)

    Mehdi Rashidi-Kouchi

    2015-06-01

    Full Text Available In this paper we proved that every g-Riesz basis for Hilbert space $H$ with respect to $K$ by adding a condition is a Riesz basis for Hilbert $B(K$-module $B(H,K$. This is an extension of [A. Askarizadeh,M. A. Dehghan, {em G-frames as special frames}, Turk. J. Math., 35, (2011 1-11]. Also, we derived similar results for g-orthonormal and orthogonal bases. Some relationships between dual frame, dual g-frame and exact frame and exact g-frame are presented too.

  16. Constitutionalizing secularism, alternative secularisms or liberal-democratic constitutionalism?A critical reading of some Turkish, ECtHR and Indian Supreme Court cases on ‘secularism’

    Directory of Open Access Journals (Sweden)

    Veit Bader

    2010-11-01

    Full Text Available In recent debates on the constitutional status of 'secularism' we can discern three positions. The first tries to overcome the absence of 'secularism' in most liberal-democratic constitutions by developing a more robust theory of constitutional secularism. The second develops theories of 'alternative secularisms'. The third, defended in this article, argues that we should drop secularism as a 'cacophonous' concept from our constitutional and legal language and replace it by liberal-democratic constitutionalism. I develop an analytical taxonomy of twelve different meanings of 'secularism' based on a comparative study of Turkish and Indian Supreme Court cases on secularism, and demonstrate that they are incompatible with each other and with the hard core of liberal-democratic constitutions. Next, I criticize the respective rulings in the Turkish and Indian context. Particularly in 'militant democracies', the appeal to a principle of 'secularism' turns out to be inimical to the liberal and to the democratic 'constitutional essentials'. I end with some normative recommendations on the role of constitutional review and judicial activism.

  17. Reading Letters

    DEFF Research Database (Denmark)

    Beier, Sofie

    2012-01-01

    In our everyday life we constantly encounter a diversity of reading matters, including display types on traffic signage, printed text in novels, newspaper headlines, or our own writing on a computer screen. All these conditions place different demands on the typefaces applied. The book discusses...... these aspects by drawing on typography history, designers’ ideas, and available scientific data concerning the reading process....

  18. Reading Aloud

    Science.gov (United States)

    Delo, Lynda

    2008-01-01

    Many resources are available to elementary teachers who wish to support science learning with literature. Unfortunately, somewhere between middle school and high school, the emphasis on using literature to teach science content--particularly the exercise of reading aloud--has all but disappeared. However, the practice of reading aloud is helpful…

  19. Framing Light Rail Projects

    DEFF Research Database (Denmark)

    Olesen, Mette

    2014-01-01

    In Europe, there has been a strong political will to implement light rail. This article contributes to the knowledge concerning policies around light rail by analysing how local actors frame light rail projects and which rationalities and arguments are present in this decision-making process....... The article draws on the socio-technical approach to mobilities studies in order to reassemble the decision-making process in three European cases: Bergen, Angers, and Bern. This article provides insights into the political, discursive and material production of light rail mobilities in a European context....... It identifies the planning rationales behind the systems and the policies that have been supportive of this light rail vision. Finally, the article identifies the practical challenges and potentials that have been connected to the different local frames of light rail mobility which can be used in future...

  20. Rigid Quasilocal Frames

    CERN Document Server

    McGrath, Paul L

    2014-01-01

    In this thesis, I examine in detail the properties of rigid quasilocal frames (RQF), which have been proposed as a geometrically natural way to define spatially extended reference frames in general relativity. I also explore their usefulness, in particular, as a tool for constructing completely general conservation laws that do not rely on the presence of spacetime symmetries and include both matter and gravitational contributions without the need for any ad hoc structures such as pseudotensors. In doing so, I show how the RQF approach affords a deeper understanding of the nature of gravitational fluxes via the equivalence principle. Finally, I apply the RQF formalism to explore Ehrenfest's rotating disk paradox, a generalization of Archimedes' law to curved spacetime, tidal interactions for Earth's and Jupiter's moons, and more.

  1. How Reading Volume Affects both Reading Fluency and Reading Achievement

    Directory of Open Access Journals (Sweden)

    Richard L. ALLINGTON

    2014-10-01

    Full Text Available Long overlooked, reading volume is actually central to the development of reading proficiencies, especially in the development of fluent reading proficiency. Generally no one in schools monitors the actual volume of reading that children engage in. We know that the commonly used commercial core reading programs provide only material that requires about 15 minutes of reading activity daily. The remaining 75 minute of reading lessons is filled with many other activities such as completing workbook pages or responding to low-level literal questions about what has been read. Studies designed to enhance the volume of reading that children do during their reading lessons demonstrate one way to enhance reading development. Repeated readings have been widely used in fostering reading fluency but wide reading options seem to work faster and more broadly in developing reading proficiencies, including oral reading fluency.

  2. Framing a Bank

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    2012-01-01

    Danish bank, Danske Bank, during the 2008 financial crisis and hence in shaping its image projected to the public. Through the study of a number of semantic frames adopted by the Danish print press and those adopted by the Bank, this article will argue for the constructions of the press putting...... considerable strain on the Bank and its image, leading it to reconsider its previous strategy of denial of responsibility...

  3. Density of Gabor Frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Heil, C.; Deng, B.

    1997-01-01

    A Gabor system is a set of time-frequency shifts$S(g,\\Lambda) = \\{e^{2\\pi i b x} g(x-a)\\}_{(a,b) \\in \\Lambda}$of a function $g \\in L^2({\\bold R}^d)$.We prove that if a finite union of Gabor systems$\\bigcup_{k=1}^r S(g_k,\\Lambda_k)$, with arbitrary sequences $\\Lambda_k$,forms a frame for $L^2({\\bo...

  4. Optical loop framing

    International Nuclear Information System (INIS)

    The ATA provides an electron beam pulse of 70-ns duration at a 1-Hz rate. Our present optical diagnostics technique involve the imaging of the visible light generated by the beam incident onto the plant of a thin sheet of material. It has already been demonstrated that the light generated has a sufficiently fast temporal reponse in performing beam diagnostics. Notwithstanding possible beam emittance degradation due to scattering in the thin sheet, the observation of beam spatial profiles with relatively high efficiencies has provided data complementary to that obtained from beam wall current monitors and from various x-ray probes and other electrical probes. The optical image sensor consists of a gated, intensified television system. The gate pulse of the image intensifier can be appropriately delayed to give frames that are time-positioned from the head to the tail of the beam with a minimum gate time of 5-ns. The spatial correlation of the time frames from pulse to pulse is very good for a stable electron beam; however, when instabilities do occur, it is difficult to properly assess the spatial composition of the head and the tail of the beam on a pulse-to-pulse basis. Multiple gating within a pulse duration becomes desirable but cannot be performed because the recycle time (20-ms) of the TV system is much longer than the beam pulse. For this reason we have developed an optical-loop framing technique that will allow the recording of two frames within one pulse duration with our present gated/intensified TV system

  5. Promoting preschool reading

    OpenAIRE

    Istenič, Vesna

    2013-01-01

    The thesis titled Promoting preschool reading consists of a theoretiral and an empirical part. In the theoretical part I wrote about reading, the importance of reading, types of reading, about reading motivation, promoting reading motivation, internal and external motivation, influence of reading motivation on the child's reading activity, reading and familial literacy, the role of adults in promotion reading literacy, reading to a child and promoting reading in pre-school years, where I ...

  6. Robotically-adjustable microstereotactic frames for image-guided neurosurgery

    Science.gov (United States)

    Kratchman, Louis B.; Fitzpatrick, J. Michael

    2013-03-01

    Stereotactic frames are a standard tool for neurosurgical targeting, but are uncomfortable for patients and obstruct the surgical field. Microstereotactic frames are more comfortable for patients, provide better access to the surgical site, and have grown in popularity as an alternative to traditional stereotactic devices. However, clinically available microstereotactic frames require either lengthy manufacturing delays or expensive image guidance systems. We introduce a robotically-adjusted, disposable microstereotactic frame for deep brain stimulation surgery that eliminates the drawbacks of existing microstereotactic frames. Our frame can be automatically adjusted in the operating room using a preoperative plan in less than five minutes. A validation study on phantoms shows that our approach provides a target positioning error of 0.14 mm, which exceeds the required accuracy for deep brain stimulation surgery.

  7. Characterization of Chlamydia trachomatis Plasmid-Encoded Open Reading Frames

    OpenAIRE

    Gong, Siqi; Yang, Zhangsheng; Lei, Lei; Shen, Li; Zhong, Guangming

    2013-01-01

    The recent success in transformation of Chlamydia trachomatis represents a major advancement in Chlamydia research. Plasmid-free C. trachomatis serovar L2 organisms can be transformed with chlamydial plasmid-based shuttle vectors pGFP::SW2 and pBRCT. Deletion of plasmid genes coding for Pgp1 to Pgp8 in pBRCT led to the identification of Pgp1, -2, -6, and -8 as plasmid maintenance factors; Pgp4 as a transcriptional regulator of chlamydial virulence-associated gene expression; and Pgp3, -5, and...

  8. VIOLENT FRAMES IN ACTION

    Energy Technology Data Exchange (ETDEWEB)

    Sanfilippo, Antonio P.; McGrath, Liam R.; Whitney, Paul D.

    2011-11-17

    We present a computational approach to radical rhetoric that leverages the co-expression of rhetoric and action features in discourse to identify violent intent. The approach combines text mining and machine learning techniques with insights from Frame Analysis and theories that explain the emergence of violence in terms of moral disengagement, the violation of sacred values and social isolation in order to build computational models that identify messages from terrorist sources and estimate their proximity to an attack. We discuss a specific application of this approach to a body of documents from and about radical and terrorist groups in the Middle East and present the results achieved.

  9. Continuous Shearlet Tight Frames

    KAUST Repository

    Grohs, Philipp

    2010-10-22

    Based on the shearlet transform we present a general construction of continuous tight frames for L2(ℝ2) from any sufficiently smooth function with anisotropic moments. This includes for example compactly supported systems, piecewise polynomial systems, or both. From our earlier results in Grohs (Technical report, KAUST, 2009) it follows that these systems enjoy the same desirable approximation properties for directional data as the previous bandlimited and very specific constructions due to Kutyniok and Labate (Trans. Am. Math. Soc. 361:2719-2754, 2009). We also show that the representation formulas we derive are in a sense optimal for the shearlet transform. © 2010 Springer Science+Business Media, LLC.

  10. Synchronization and Inertial Frames

    CERN Document Server

    Viazminsky, C P

    1999-01-01

    In classical mechanics, a procedure for simultaneous synchronization in all inertial frames is consistent with the Galilean transformation. However, if one attempts to achieve such a synchronization utilizing light signals, he will be facing in the first place the break down of simultaneity, and secondly, a self-contradictory transformation that has the Lorentz transformation, or its confinement to the velocity of light, as the only possible ways that resolve the contradiction. The current work constitutes a smooth transition from traditional to relativistic vision of mechanics, and therefore is quite appealing from pedagogical point of view.

  11. Reading Aloud.

    Science.gov (United States)

    Giorgis, Cyndi; Johnson, Nancy J.

    1999-01-01

    Offers brief descriptions of 34 children's books that are excellent for reading aloud: some of them for inviting interaction, for laughing out loud, for prompting discussion, for living vicariously, for lingering over language, and for making curricular connections. (SR)

  12. Fiber Bundles and Parseval Frames

    OpenAIRE

    Agrawal, Devanshu; Knisley, Jeff

    2015-01-01

    Continuous frames over a Hilbert space have a rich and sophisticated structure that can be represented in the form of a fiber bundle. The fiber bundle structure reveals the central importance of Parseval frames and the extent to which Parseval frames generalize the notion of an orthonormal basis.

  13. Reference frame for Product Configuration

    DEFF Research Database (Denmark)

    Ladeby, Klaes Rohde; Oddsson, Gudmundur Valur

    2011-01-01

    This paper presents a reference frame for configuration. The reference frame is established by review of existing literature, and consequently it is a theoretical frame of reference. The review of literature shows a deterioration of the understanding of configuration. Most recent literature reports...

  14. New avenues for framing research

    NARCIS (Netherlands)

    C.H. de Vreese

    2012-01-01

    In this article, the author reviews the studies in this special issue of the American Behavioral Scientist. It is a strong collection of articles reporting findings from an integrated project that looks at frame building, frames, and effects of frames. The project is part of an exciting large-scale

  15. A review of the status of alternative approaches to animal testing and the development of integrated testing strategies for assessing the toxicity of chemicals under REACH--a summary of a DEFRA-funded project conducted by Liverpool John Moores University and FRAME.

    Science.gov (United States)

    Grindon, Christina; Combes, Robert; Cronin, Mark T D; Roberts, David W; Garrod, John

    2006-03-01

    Liverpool John Moores University and FRAME were recently awarded a DEFRA tender to conduct a review of the status of alternative approaches to animal testing, and to recommend further research with regard to the forthcoming European Union REACH (Registration, Evaluation and Authorisation of Chemicals) system for the safety testing and risk assessment of chemicals. The outcome of the project is summarised, including the prospects for in vitro and in silico testing, areas where reduction and refinement could be applied, and how decision-tree integrated testing strategies could be used to reduce the number of animals needed to fulfil the testing requirements of the REACH system. This paper is a prelude to a series of individual papers on detailed suggestions for applying non-animal methods to each of the major toxicity endpoints in REACH. PMID:16555968

  16. Frame by frame stop motion non-traditional approaches to stop motion animation

    CERN Document Server

    Gasek, Tom

    2011-01-01

    In a world that is dominated by computer images, alternative stop motion techniques like pixilation, time-lapse photography and down-shooting techniques combined with new technologies offer a new, tangible and exciting approach to animation. With over 25 years professional experience, industry veteran, Tom Gasek presents a comprehensive guide to stop motion animation without the focus on puppetry or model animation. With tips, tricks and hands-on exercises, Frame by Frame will help both experienced and novice filmmakers get the most effective results from this underutilized branch of animation

  17. Effects of the deletion of early region 4 (E4) open reading frame 1 (orf1), orf1-2, orf1-3 and orf1-4 on virus-host cell interaction, transgene expression, and immunogenicity of replicating adenovirus HIV vaccine vectors.

    Science.gov (United States)

    Thomas, Michael A; Song, Rui; Demberg, Thorsten; Vargas-Inchaustegui, Diego A; Venzon, David; Robert-Guroff, Marjorie

    2013-01-01

    The global health burden engendered by human immunodeficiency virus (HIV)-induced acquired immunodeficiency syndrome (AIDS) is a sobering reminder of the pressing need for a preventative vaccine. In non-human primate models replicating adenovirus (Ad)-HIV/SIV recombinant vaccine vectors have been shown to stimulate potent immune responses culminating in protection against challenge exposures. Nonetheless, an increase in the transgene carrying capacity of these Ad vectors, currently limited to approximately 3000 base pairs, would greatly enhance their utility. Using a replicating, E3-deleted Ad type 5 host range mutant (Ad5 hr) encoding full-length single-chain HIVBaLgp120 linked to the D1 and D2 domains of rhesus macaque CD4 (rhFLSC) we systematically deleted the genes encoding early region 4 open reading frame 1 (E4orf1) through E4orf4. All the Ad-rhFLSC vectors produced similar levels of viral progeny. Cell cycle analysis of infected human and monkey cells revealed no differences in virus-host interaction. The parental and E4-deleted viruses expressed comparable levels of the transgene with kinetics similar to Ad late proteins. Similar levels of cellular immune responses and transgene-specific antibodies were elicited in vaccinated mice. However, differences in recognition of Ad proteins and induced antibody subtypes were observed, suggesting that the E4 gene products might modulate antibody responses by as yet unknown mechanisms. In short, we have improved the transgene carrying capacity by one thousand base pairs while preserving the replicability, levels of transgene expression, and immunogenicity critical to these vaccine vectors. This additional space allows for flexibility in vaccine design that could not be obtained with the current vector and as such should facilitate the goal of improving vaccine efficacy. To the best of our knowledge, this is the first report describing the effects of these E4 deletions on transgene expression and immunogenicity in a

  18. Effects of news frames on perceived risk, emotions, and learning.

    Directory of Open Access Journals (Sweden)

    Christine Otieno

    Full Text Available The media play a key role in forming opinions by influencing people´s understanding and perception of a topic. People gather information about topics of interest from the internet and print media, which employ various news frames to attract attention. One example of a common news frame is the human-interest frame, which emotionalizes and dramatizes information and often accentuates individual affectedness. Our study investigated effects of human-interest frames compared to a neutral-text condition with respect to perceived risk, emotions, and knowledge acquisition, and tested whether these effects can be "generalized" to common variants of the human-interest frame. Ninety-one participants read either one variant of the human-interest frame or a neutrally formulated version of a newspaper article describing the effects of invasive species in general and the Asian ladybug (an invasive species in particular. The framing was achieved by varying the opening and concluding paragraphs (about invasive species, as well as the headline. The core text (about the Asian ladybug was the same across all conditions. All outcome variables on framing effects referred to this common core text. We found that all versions of the human-interest frame increased perceived risk and the strength of negative emotions compared to the neutral text. Furthermore, participants in the human-interest frame condition displayed better (quantitative learning outcomes but also biased knowledge, highlighting a potential dilemma: Human-interest frames may increase learning, but they also lead to a rather unbalanced view of the given topic on a "deeper level".

  19. Mapping in inertial frames

    Energy Technology Data Exchange (ETDEWEB)

    Arunasalam, V.

    1989-05-01

    World space mapping in inertial frames is used to examine the Lorentz covariance of symmetry operations. It is found that the Galilean invariant concepts of simultaneity (S), parity (P), and time reversal symmetry (T) are not Lorentz covariant concepts for inertial observers. That is, just as the concept of simultaneity has no significance independent of the Lorentz inertial frame, likewise so are the concepts of parity and time reversal. However, the world parity (W) (i.e., the space-time reversal symmetry (P-T)) is a truly Lorentz covariant concept. Indeed, it is shown that only those mapping matrices M that commute with the Lorentz transformation matrix L (i.e., (M,L) = 0) are the ones that correspond to manifestly Lorentz covariant operations. This result is in accordance with the spirit of the world space Mach's principle. Since the Lorentz transformation is an orthogonal transformation while the Galilean transformation is not an orthogonal transformation, the formal relativistic space-time mapping theory used here does not have a corresponding non-relativistic counterpart. 12 refs.

  20. Mapping in inertial frames

    International Nuclear Information System (INIS)

    World space mapping in inertial frames is used to examine the Lorentz covariance of symmetry operations. It is found that the Galilean invariant concepts of simultaneity (S), parity (P), and time reversal symmetry (T) are not Lorentz covariant concepts for inertial observers. That is, just as the concept of simultaneity has no significance independent of the Lorentz inertial frame, likewise so are the concepts of parity and time reversal. However, the world parity (W) [i.e., the space-time reversal symmetry (P-T)] is a truly Lorentz covariant concept. Indeed, it is shown that only those mapping matrices M that commute with the Lorentz transformation matrix L (i.e., [M,L] = 0) are the ones that correspond to manifestly Lorentz covariant operations. This result is in accordance with the spirit of the world space Mach's principle. Since the Lorentz transformation is an orthogonal transformation while the Galilean transformation is not an orthogonal transformation, the formal relativistic space-time mapping theory used here does not have a corresponding non-relativistic counterpart. 12 refs

  1. Semiclassical framed BPS states

    CERN Document Server

    Moore, Gregory W; Bleeken, Dieter Van den

    2015-01-01

    We provide a semiclassical description of framed BPS states in four-dimensional N = 2 super Yang-Mills theories probed by 't Hooft defects, in terms of a supersymmetric quantum mechanics on the moduli space of singular monopoles. Framed BPS states, like their ordinary counterparts in the theory without defects, are associated with the L^2 kernel of certain Dirac operators on moduli space, or equivalently with the L^2 cohomology of related Dolbeault operators. The Dirac/Dolbeault operators depend on two Cartan-valued Higgs vevs. We conjecture a map between these vevs and the Seiberg-Witten special coordinates, consistent with a one-loop analysis and checked in examples. The map incorporates all perturbative and nonperturbative corrections that are relevant for the semiclassical construction of BPS states, over a suitably defined weak coupling regime of the Coulomb branch. We use this map to translate wall crossing formulae and the no exotics theorem to statements about the Dirac/Dolbeault operators. The no exo...

  2. How Reading Volume Affects both Reading Fluency and Reading Achievement

    OpenAIRE

    Richard L. ALLINGTON

    2014-01-01

    Long overlooked, reading volume is actually central to the development of reading proficiencies, especially in the development of fluent reading proficiency. Generally no one in schools monitors the actual volume of reading that children engage in. We know that the commonly used commercial core reading programs provide only material that requires about 15 minutes of reading activity daily. The remaining 75 minute of reading lessons is filled with many other activities such as comp...

  3. Does E-Reading Enhance Reading Fluency?

    Science.gov (United States)

    Akbar, Rahima S.; Taqi, Hanan A.; Dashti, Abdulmohsin A.; Sadeq, Taiba M.

    2015-01-01

    Extensive reading is reading as much as possible, for one's own pleasure, at a difficulty level at which one can read smoothly and quickly. In the domain of reading, this paper investigates the effect of extensive reading from e-books, through utilizing a number of downloadable reading application programs on the students' e-devices, as opposed to…

  4. Frame architecture for video servers

    Science.gov (United States)

    Venkatramani, Chitra; Kienzle, Martin G.

    1999-11-01

    Video is inherently frame-oriented and most applications such as commercial video processing require to manipulate video in terms of frames. However, typical video servers treat videos as byte streams and perform random access based on approximate byte offsets to be supplied by the client. They do not provide frame or timecode oriented API which is essential for many applications. This paper describes a frame-oriented architecture for video servers. It also describes the implementation in the context of IBM's VideoCharger server. The later part of the paper describes an application that uses the frame architecture and provides fast and slow-motion scanning capabilities to the server.

  5. Equiangular Frames and Signature Sets

    CERN Document Server

    Singh, Preeti

    2009-01-01

    We will present a relation between real equiangular frames and certain special sets in groups which we call signature sets and show that many equiangular frames arise in this manner. Then we will define quasi-signature sets and will examine equiangular frames associated to these subsets of groups. We will extend these results to complex equiangular frames where the inner product between any pair of vectors is a common multiple of a cube root of unity and exhibit equiangular frames that arise from groups in this manner.

  6. Reading critically and reading creatively

    OpenAIRE

    Henry Jenkins

    2012-01-01

    This text discusses the importance of fiction derived from literary works by their fans,proposing the difference between critical reading and creative reading. He explores themain methods used and the motivation of this subculture, as well as its manifestationon the Internet. He not only cites some commercial works based on classics, but alsodiscusses the possible pedagogical impact of appropriating of this phenomenon inthe classroom.

  7. Reading critically and reading creatively

    Directory of Open Access Journals (Sweden)

    Henry Jenkins

    2012-12-01

    Full Text Available This text discusses the importance of fiction derived from literary works by their fans,proposing the difference between critical reading and creative reading. He explores themain methods used and the motivation of this subculture, as well as its manifestationon the Internet. He not only cites some commercial works based on classics, but alsodiscusses the possible pedagogical impact of appropriating of this phenomenon inthe classroom.

  8. How Reading Volume Affects Both Reading Fluency and Reading Achievement

    Science.gov (United States)

    Allington, Richard L.

    2014-01-01

    Long overlooked, reading volume is actually central to the development of reading proficiencies, especially in the development of fluent reading proficiency. Generally no one in schools monitors the actual volume of reading that children engage in. We know that the commonly used commercial core reading programs provide only material that requires…

  9. Scarcity frames value.

    Science.gov (United States)

    Shah, Anuj K; Shafir, Eldar; Mullainathan, Sendhil

    2015-04-01

    Economic models of decision making assume that people have a stable way of thinking about value. In contrast, psychology has shown that people's preferences are often malleable and influenced by normatively irrelevant contextual features. Whereas economics derives its predictions from the assumption that people navigate a world of scarce resources, recent psychological work has shown that people often do not attend to scarcity. In this article, we show that when scarcity does influence cognition, it renders people less susceptible to classic context effects. Under conditions of scarcity, people focus on pressing needs and recognize the trade-offs that must be made against those needs. Those trade-offs frame perception more consistently than irrelevant contextual cues, which exert less influence. The results suggest that scarcity can align certain behaviors more closely with traditional economic predictions. PMID:25676256

  10. Voz sobre frame relay

    OpenAIRE

    D´Elia, Gabriel Anibal

    2000-01-01

    Esta tesis trata el tema de VOFR, desde la digitalización de la voz hasta su transmisión a través de dicha red, así también como la comparación con otros medios de transporte como VOIP. Dada las características del protocolo frame relay y su disponibilidad se eligió como el medio más apropiado para la transmisión de voz y datos en forma integrada sobre una misma red. El trabajo comienza con una breve explicación de la voz, su digitalización y forma actual de transmisión a través de una red di...

  11. FishFrame

    DEFF Research Database (Denmark)

    Degel, Henrik; Jansen, Teunis

    2006-01-01

    Many research and advisory tasks needs quality assured data on a disaggregated level to make the re-stratification needed to answer a specific question. It is a slow process to gather international datasets for these analyses, because the raw data are spread between labs. Even when gathering data...... for fixed reoccurring tasks like assessment working groups, data are often late and the quality can be unsatisfactory. The current situation of this “semi-manual distributed datawarehouse” can be improved technically. Methods for quality control, raising and calculation can be discussed and unified....... Development and test of software modules can be done once and reused by all. The biggest challenge in this is not technical – it is in organisation, coordination and trust. This challenge has been addressed by FishFrame - a web-based datawarehouse application. The “bottom-up” approach with maximum involvement...

  12. Language use depending on news frame and immigrant origin.

    Science.gov (United States)

    Fernández, Itziar; Igartua, Juan-José; Moral, Félix; Palacios, Elena; Acosta, Tania; Muñoz, Dolores

    2013-01-01

    The purpose of this study was to analyze the effect of the media on individuals' specific language use in relation to a news story on immigration: the influence of the news frame and group cue. Abstraction, complexity of language use, and negative affective language were evaluated. The 523 participants were randomly distributed to each of the four experimental conditions: news frame (crime versus economic contribution) by group cue (geographical origin of the immigrants involved: Moroccans versus Latin Americans). Through content analysis of the ideas and reflections that arose after the participants read the different news stories, using the Linguistic Category Model (LCM; Semin & Fiedler, 1991) to measure abstract language and the Linguistic Inquiry and Word Count (LIWC; Pennebaker, Booth, & Francis, 2007) to analyze complex language and negative affective language, it emerged that abstract language and negative affective language were more frequent in the participants assigned to the news frame on crime. Complex language was more commonly used when the news frame referred to the economic contribution of immigrants. Regression analyses showed the mediating role of attitude to immigration in the effects of news frame on negative affective language. The bootstrap method was used to assess the magnitude of the indirect effect. A significant mediator effect was also found through structural equation modeling. Analyses of covariance showed one interaction between news frame and group cue: Among those who read the news story in a frame linking immigration to crime and Moroccan origin, abstract language was more characteristic. The results are discussed from the theoretical perspective of framing. PMID:23113573

  13. Seismic Performance Comparison of a High-Content SDA Frame and Standard RC Frame

    Directory of Open Access Journals (Sweden)

    John W. van de Lindt

    2011-01-01

    Full Text Available This study presents the method and results of an experiment to study the seismic behavior of a concrete portal frame with fifty percent of its cement content replaced with a spray dryer ash (SDA. Based on multiple-shake-table tests, the high content SDA frame was found to perform as well as the standard concrete frame for two earthquakes exceeding design-level intensity earthquakes. Hence, from a purely seismic/structural standpoint, it may be possible to replace approximately fifty percent of cement in a concrete mix with SDA for the construction of structural members in high seismic zones. This would help significantly redirect spray dryer ash away from landfills, thus, providing a sustainable greener alternative to concrete that uses only Portland cement, or only a small percentage of SDA or fly ash.

  14. The Duals of Fusion Frames for Experimental Data Transmission Coding of High Energy Physics

    OpenAIRE

    Jinsong Leng; Qixun Guo; Tingzhu Huang

    2013-01-01

    The experimental data transmission is an important part of high energy physics experiment. In this paper, we connect fusion frames with the experimental data transmission implement of high energy physics. And we research the utilization of fusion frames for data transmission coding which can enhance the transmission efficiency, robust against erasures, and so forth. For this application, we first characterize a class of alternate fusion frames which are duals of a given fusion frame in a Hilb...

  15. Riesz frames and approximation of the frame coefficients

    DEFF Research Database (Denmark)

    Casazza, P.; Christensen, Ole

    1998-01-01

    A frame is a fmaily {f i } i=1 ∞ of elements in a Hilbert space with the property that every element in can be written as a (infinite) linear combination of the frame elements. Frame theory describes how one can choose the corresponding coefficients, which are called frame coefficients. From...... avoided the inversion problem. In the same spirit we approximate the solution to a moment problem. It turns out, that the class of “well-behaving frames” are identical for the two problems we consider....

  16. Riesz Frames and Approximation of the Frame Coefficients

    DEFF Research Database (Denmark)

    Christensen, Ole

    1996-01-01

    A frame is a familyof elements in a Hilbert space with the propertythat every element in the Hilbert space can be written as a (infinite)linear combination of the frame elements. Frame theory describes howone can choose the corresponding coefficients, which are calledframe coefficients. From...... the mathematical point of view this isgratifying, but for applications it is a problem that the calculationrequires inversion of an operator on the Hilbert space.The projection method is introduced in order to avoid this problem.The basic idea is to consider finite subfamiliesof the frame and the orthogonal...

  17. APPLYING PQ4R STRATEGY FOR TEACHING READING

    OpenAIRE

    Moh Rodli

    2015-01-01

    Despite receiving more attention than other language skills in English classroom, the result of students’ reading comprehension achievement is still far from expectation. To help students comprehend English reading texts and have better achievement in reading skill, PQ4R strategy can be an alternative to offer. The PQ4R (also well known as SQ4R) strategy of comprehending reading material is an extension of SQ3R (Survey, Question, Read, Recite, and Review) This method involves previewing the r...

  18. Oral Reading Fluency in Second Language Reading

    Science.gov (United States)

    Jeon, Eun Hee

    2012-01-01

    This study investigated the role of oral reading fluency in second language reading. Two hundred and fifty-five high school students in South Korea were assessed on three oral reading fluency (ORF) variables and six other reading predictors. The relationship between ORF and other reading predictors was examined through an exploratory factor…

  19. Does Extensive Reading Promote Reading Speed?

    Science.gov (United States)

    He, Mu

    2014-01-01

    Research has shown a wide range of learning benefits accruing from extensive reading. Not only is there improvement in reading, but also in a wide range of language uses and areas of language knowledge. However, few research studies have examined reading speed. The existing literature on reading speed focused on students' reading speed…

  20. Reading Clubs, Workshops, Reading in the Park

    OpenAIRE

    Banaurs, Daniela

    2013-01-01

    Reading clubs are networks made up of book lovers who read the same book at the same time and then share their ideas and feelings inspired by that reading. This short paper discusses the benefits of reading clubs and reading workshops.

  1. Reading Rembrandt

    NARCIS (Netherlands)

    Bal, Mieke

    2006-01-01

    Reading Rembrandt: Beyond the Word-Image Opposition explores the potential for an interdisciplinary methodology between visual art and literature. In a series of close analyses of works by "Rembrandt" - works as we see them today, through all the ways of seeing and commenting that precede - and text

  2. Reading Hygiene

    Institute of Scientific and Technical Information of China (English)

    耿一铭

    2006-01-01

    Here are some good points for good eye health that everyone can follow: 1.Rest your eyes before they get tired. Just close your eyes from time to time or look offat some distant object. 2.Do not read in either too dark a light or

  3. Relative Framing of Transverse knots

    OpenAIRE

    Chernov, Vladimir

    2001-01-01

    It is well-known that a knot in a contact manifold $(M,C)$ transverse to a trivialized contact structure possesses the natural framing given by the first of the trivialization vectors along the knot. If the Euler class $e_C\\in H^2(M)$ of $C$ is nonzero, then $C$ is nontrvivializable and the natural framing of transverse knots does not exist. We construct a new framing-type invariant of transverse knots called relative framing. It is defined for all tight $C$, all closed irreducible atoroidal ...

  4. WHAT IS READING?

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Reading is enjoying,entertaining and ent ightening Reading is listening,speaking and writingReading is talking and discussing,with yourself with the author and with the others Reading is exploring,investigating and guessing. Reading is traveling backward and forward,historically and geographically. Reading is thinking in your own language,and/or in the other language. Reading is encoding and decoding. Reading is civilizing,rationalizing and intellectualizing. Reading is assimilating,associating,accumula...

  5. Phonological Constraints on the Assembly of Skeletal Structure in Reading

    Science.gov (United States)

    Marom, Michal; Berent, Iris

    2010-01-01

    Linguistic research suggests that certain skeletal frames (e.g., CVC) are preferred to others (e.g., VCC). We examine whether such preferences constrain reading in the Stroop task. We demonstrate that CCVC nonwords facilitate naming the color "black" (/blaek/, a CCVC frame) relative to CVC controls. Conversely, CCVC items inhibit "red" (a CVC…

  6. Seismic behavior and design of wall-EDD-frame systems

    Directory of Open Access Journals (Sweden)

    Oren eLavan

    2015-06-01

    Full Text Available Walls and frames have different deflection lines and, depending on the seismic mass they support, may often poses different natural periods. In many cases, wall-frame structures present an advantageous behavior. In these structures the walls and the frames are rigidly connected. Nevertheless, if the walls and the frames were not rigidly connected, an opportunity for an efficient passive control strategy would arise: Connecting the two systems by energy dissipation devices (EDDs to result in wall-EDD-frame systems. This, depending on the parameters of the system, is expected to lead to an efficient energy dissipation mechanism.This paper studies the seismic behavior of wall-EDD-frame systems in the context of retrofitting existing frame structures. The controlling non-dimensional parameters of such systems are first identified. This is followed by a rigorous and extensive parametric study that reveals the pros and cons of the new system versus wall-frame systems. The effect of the controlling parameters on the behavior of the new system are analyzed and discussed. Finally, tools are given for initial design of such retrofitting schemes. These enable both choosing the most appropriate retrofitting alternative and selecting initial values for its parameters.

  7. FRAMES and Other IEM Technologies

    Science.gov (United States)

    A presentation package is developed that describes the FRAMES software technology system. The philosophy of FRAMES is discussed; its components and editors are reviewed; its relationship to integrated environmental modeling technologies; such as D4EM and SuperMUSE, are described;...

  8. On framed quantum principal bundles

    CERN Document Server

    Durdevic, M

    1995-01-01

    A noncommutative-geometric formalism of framed principal bundles is sketched, in a special case of quantum bundles (over quantum spaces) possessing classical structure groups. Quantum counterparts of torsion operators and Levi-Civita type connections are analyzed. A construction of a natural differential calculus on framed bundles is described. Illustrative examples are presented.

  9. Pairs of dual periodic frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Goh, Say Song

    2012-01-01

    needed. The purpose of the present paper is to provide constructions of dual pairs of frames in the setting of the Hilbert space of periodic functions L2(0,2π). The frames constructed are given explicitly as trigonometric polynomials, which allows for an efficient calculation of the coefficients in the...

  10. The Practicality of Poetry: Using Sonnets to Improve Reading Ability

    Science.gov (United States)

    Vallicelli, Arthur E.

    2012-01-01

    This dissertation examines the way in which sonnets improved reading comprehension through a case study. The study was conceptually framed around the ideas of the New Critics, who employed two particular fallacies to ensure the most accurate reading of literature. The purpose of the study was to see the potential benefits that could come from…

  11. Frames and outer frames for Hilbert C^*-modules

    OpenAIRE

    Arambašić, Ljiljana; Bakić, Damir

    2015-01-01

    The goal of the present paper is to extend the theory of frames for countably generated Hilbert $C^*$-modules over arbitrary $C^*$-algebras. In investigating the non-unital case we introduce the concept of outer frame as a sequence in the multiplier module $M(X)$ that has the standard frame property when applied to elements of the ambient module $X$. Given a Hilbert $\\A$-module $X$, we prove that there is a bijective correspondence of the set of all adjointable surjections from the generalize...

  12. EMPOWERING THE READING READABILITY

    OpenAIRE

    Handoko Handoko

    2014-01-01

    A general assumption about reading is that students improve their reading ability by reading a lot. This research was conducted to explain the use of extensive reading and aimed to figure out its implementation in improving students’ reading readability by using the class action research technique. The data of this research relates to the students ‘reading progress shown in their reading reports: spoken and written summary, reading comprehension and vocabulary mastery and their participation....

  13. Framed School--Frame Factors, Frames and the Dynamics of Social Interaction in School

    Science.gov (United States)

    Persson, Anders

    2015-01-01

    This paper aims to show how the Goffman frame perspective can be used in an analysis of school and education and how it can be combined, in such analysis, with the frame factor perspective. The latter emphasizes factors that are determined outside the teaching process, while the former stresses how actors organize their experiences and define…

  14. Framing Obesity: How News Frames Shape Attributions and Behavioral Responses.

    Science.gov (United States)

    Sun, Ye; Krakow, Melinda; John, Kevin K; Liu, Miao; Weaver, Jeremy

    2016-01-01

    Based on a public health model of obesity, this study set out to examine whether a news article reporting the obesity issue in a societal versus individual frame would increase perceptions of societal responsibilities for the obesity problem and motivate responsibility-taking behaviors. Responsibility-taking behaviors were examined at 3 levels: personal, interpersonal, and societal. Data from a Web-based experiment revealed significant framing effects on behaviors via causal and treatment responsibility attributions. The societal frame increased societal causal and treatment attribution, which led to greater likelihoods of interpersonal and social responsibility-taking behaviors as well as personal behaviors. Our findings suggest that news framing can be an effective venue for raising awareness of obesity as a societal issue and mobilizing collective efforts. PMID:26375052

  15. The Art of Frame Theory

    OpenAIRE

    Casazza, Peter G.

    1999-01-01

    Thye theory of frames for a Hilbert space plays a fundamental role in signal processing, image processing, data compression, sampling theory and much more, as well as being a fruitful area of research in abstract mathematics. In this ``tutorial'' on abstract frame theory, we will try to point out the major directions of research in abstract frame theory and give some sample techniques from each of the areas. We will also bring out some of the important open questions, discuss some of the limi...

  16. Reading Together: A Successful Reading Fluency Intervention

    Science.gov (United States)

    Young, Chase; Mohr, Kathleen A. J.; Rasinski, Timothy

    2015-01-01

    The article describes a reading fluency intervention called Reading Together that combines the method of repeated readings (Samuels, 1979) and the Neurological Impress Method (Heckelman, 1969). Sixteen volunteers from various backgrounds were recruited and trained to deliver the Reading Together intervention to struggling readers in third through…

  17. Pleasure Reading and Reading Rate Gains

    Science.gov (United States)

    Beglar, David; Hunt, Alan

    2014-01-01

    This study investigated the effects of (a) the amount of pleasure reading completed, (b) the type of texts read (i.e., simplified or unsimplified books), and (c) the level of simplified texts read by 14 Japanese university students who made the largest reading rate gains over one academic year. The findings indicated that the participants who made…

  18. Co-reading: investigating collaborative group reading.

    OpenAIRE

    Pearson, J.; Owen, T.; Thimbleby, H. W.; Buchanan, G.

    2012-01-01

    Collaborative reading, or co-reading as we call it, is ubiquitous—it occurs, for instance, in classrooms, book-clubs, and in less coordinated ways through mass media. While individual digital reading has been the subject of much investigation, research into co-reading is scarce. We report a two-phase field study of group reading to identify an initial set of user requirements. A co-reading interface is then designed that facilitates the coordination of group reading by providing temporary ‘Po...

  19. Framing the patent troll debate.

    Science.gov (United States)

    Risch, Michael

    2014-02-01

    The patent troll debate has reached a fevered pitch in the USA. This editorial seeks to frame the debate by pointing out the lack of clarity in defining patent trolls and their allegedly harmful actions. It then frames the debate by asking currently unanswered questions: Where do troll patents come from? What are the effects of troll assertions? Will policy changes improve the system? PMID:24354803

  20. Willpower depletion and framing effects

    OpenAIRE

    de Haan, Thomas; van Veldhuizen, Roel

    2013-01-01

    We investigate whether depleting people's cognitive resources (or willpower) affects the degree to which they are susceptible to framing effects. Recent research in social psychology and economics has suggested that willpower is a resource that can be temporarily depleted and that a depleted level of willpower is associated with self-control problems in a variety of contexts. In this study, we extend the willpower depletion paradigm to framing effects and argue that willpower depletion should...

  1. Approximation of the Inverse -Frame Operator

    Indian Academy of Sciences (India)

    M R Abdollahpour; A Najati

    2011-05-01

    In this paper, we introduce the concept of (strong) projection method for -frames which works for all conditional -Riesz frames. We also derive a method for approximation of the inverse -frame operator which is efficient for all -frames. We show how the inverse of -frame operator can be approximated as close as we like using finite-dimensional linear algebra.

  2. Differential expression of X chromosome open reading frame 6 in hypospadiac male rats induced by Di-n-butyl phthalate%X染色体开放读码框架6在邻苯二甲酸二丁酯致雄性大鼠尿道下裂发生中的表达

    Institute of Scientific and Technical Information of China (English)

    魏俊峰; 周耀军; 王勇; 张力峰; 张炜

    2012-01-01

    Objective To verify the differential expression of X chromosome open reading frame 6 (CXorf6) in the testes of hypospadiac rats induced by maternal exposure to Di-n-butyl phthalate (DBP) and normal control rats in order to further explore the mechanism of hypospadias.Methods Twenty pregnant SD rats were randomly divided into two groups which were given DBP and soybean oil gavage at the dose of 800 mg/(kg·day) and 2 ml/day separately during the 14-18th day of pregnancy.Hypospadias incidence was calculated at GD19.The expression of CXorf6 was analyzed by Western blotting and immunohistochemistry.Results The incidence of hypospadias was 40.7%.There were statistically significant difference in the expression of CXorf6 between experiment group (0.323 ± 0.014) and control group (0.706 ±0.016,P <0.05).CXorf6 was mainly located in sertoli and leydig cells,and staining intensity in control group was stronger than in experimental group.Conclusion DBP could affect the occurrence of hypospadias by regnlating the expression of CXorf6 in fetal male rats.%目的 观察X染色体开放读码框架6(CXorf6)在邻苯二甲酸二丁酯(DBP)致大鼠尿道下裂发生中的作用.方法 于妊娠14~18 d(GD14~18)实验和对照组各10只孕鼠分别给予DBP 800 mg/(kg·d)和豆油2 ml/d灌胃,GD19剖宫产统计尿道下裂发生率,免疫印迹法和免疫组织化学法分析两组子鼠睾丸中CXorf6的表达.结果 尿道下裂仅实验组发生,发生率为40.7%;CXorf6在尿道下裂组的相对表达量为0.323±0.014(n=10),对照组为0.706±0.016(n=10),差异有统计学意义(P<0.05);CXorf6主要定位于睾丸间质和支持细胞,尿道下裂组CXorf6着色细胞数量减少,染色强度弱于对照组.结论 DBP通过干预CXorf6的表达以影响睾丸的发育及雄激素的产生而导致尿道下裂的发生.

  3. Reading with Maggie: The Effect of the Presence/Absence of a Classroom Pet Dog in a Reading Intervention Package

    Science.gov (United States)

    Bassette, Laura A.

    2011-01-01

    The purpose of this study was to examine if the presence/absence of a classroom pet dog impacted reading skills in four 5th grade middle school students with emotional/ behavioral disabilities. An alternating treatment design was used to assess the fluency and comprehension measures in students during reading a reading intervention package…

  4. Designing reading tasks to foster critical thinking Designing reading tasks to foster critical thinking

    OpenAIRE

    Leda Maria Braga Tomitch

    2008-01-01

    What else is there besides multiple choice exercises, questions or true-false statements? What kind of readers do we EFL/ESL teachers, want to develop? Fortunately, there are alternative possibilities for designing reading activities which, besides being more interesting and fun for the students than the traditional tasks, can help develop more active and critical readers. A search in EFL/ESL reading textbooks has revealed that the most frequently encountered types of reading activi...

  5. Alternative security

    International Nuclear Information System (INIS)

    This book contains the following chapters: The Military and Alternative Security: New Missions for Stable Conventional Security; Technology and Alternative Security: A Cherished Myth Expires; Law and Alternative Security: Toward a Just World Peace; Politics and Alternative Security: Toward a More Democratic, Therefore More Peaceful, World; Economics and Alternative Security: Toward a Peacekeeping International Economy; Psychology and Alternative Security: Needs, Perceptions, and Misperceptions; Religion and Alternative Security: A Prophetic Vision; and Toward Post-Nuclear Global Security: An Overview

  6. Approach/Avoidance Motivation, Message Framing, and Health Behavior: Understanding the Congruency Effect.

    Science.gov (United States)

    Sherman, David K; Mann, Traci; Updegraff, John A

    2006-06-01

    Health messages framed to be congruent with individuals' approach/avoidance motivations have been found to be more effective in promoting health behaviors than health messages incongruent with approach/avoidance motivations. This study examines the processes underlying this congruency effect. Participants (undergraduate students, N = 67) completed a measure of approach/avoidance orientation (the BIS/BAS scales) and read either a gain- or loss-framed message promoting dental flossing. Results demonstrated a congruency effect: Participants who read a congruently framed message had greater flossing efficacy, intended to floss more, and used more dental flosses than did the participants who read an incongruent message. Moreover, intention to perform the behavior predicted the congruency effect and self-efficacy mediated participants' intentions to perform the health behavior. Discussion centers on the role of personality factors and situational factors in models of behavior change. PMID:19079797

  7. Beyond Cognition: Reading Motivation and Reading Comprehension

    Science.gov (United States)

    Wigfield, Allan; Gladstone, Jessica; Turci, Lara

    2016-01-01

    The authors review research on children’s reading motivation and its relation to their reading comprehension. They begin by discussing work on the development of school motivation in general and reading motivation in particular, reviewing work showing that many children’s reading motivation declines over the school years. Girls tend to have more positive motivation for reading than do boys, and there are ethnic differences in children’s reading motivation. Over the last 15 years researchers have identified in both laboratory and classroom-based research instructional practices that positively impact students’ reading motivation and ultimately their reading comprehension. There is a strong need for researchers to build on this work and develop and study in different age groups of children effective classroom-based reading motivation instructional programs for a variety of narrative and informational materials.

  8. Preserved stenography reading in a patient with pure alexia. A model for dissociated reading processes.

    Science.gov (United States)

    Regard, M; Landis, T; Hess, K

    1985-04-01

    A proficient stenographer who had had cerebral metastases suffered from pure alexia for normal print but could still read stenography with ease. It is suggested that especially the visuospatial properties of stenography made possible "alternative" reading, most likely via the right hemisphere. PMID:3985817

  9. *-Frames for operators on Hilbert modules

    OpenAIRE

    Janfada, Mohammad; Dastourian, Bahram

    2014-01-01

    K-frames were introduced by L. Gavruta to study atomic systems on Hilbert spaces. Recently some generalizations of this concept are introduced and some of its difference with ordinary frames are studied. In this paper *-K-frames are introduced and some properties of this generalization of K-frames are studied. After proving some characterizations of *-K-frames, direct sum and tensor product of *-K-frames are considered and finally some perturbation results are established.

  10. Frame theory for binary vector spaces

    OpenAIRE

    Bodmann, Bernhard G.; Le, My; Reza, Letty; Tobin, Matthew; Tomforde, Mark

    2009-01-01

    We develop the theory of frames and Parseval frames for finite-dimensional vector spaces over the binary numbers. This includes characterizations which are similar to frames and Parseval frames for real or complex Hilbert spaces, and the discussion of conceptual differences caused by the lack of a proper inner product on binary vector spaces. We also define switching equivalence for binary frames, and list all equivalence classes of binary Parseval frames in lowest dimensions, excluding cases...

  11. Some properties of C-fusion frames

    OpenAIRE

    Faroughi , Mohammad Hasan

    2010-01-01

    In [10], we generalized the concept of fusion frames, namely, c-fusion frames, which is a continuous version of the fusion frames. In this article we give some important properties about the generalization, namely erasures of subspaces, the bound of c-erasure reconstruction error for Parseval c-fusion frames, perturbation of c-fusion frames and the frame operator for fusion pair.

  12. Rearing a reading habit

    OpenAIRE

    M.S. Sridhar

    2009-01-01

    Discusses the importance and ways of inculcating reading habit in children at the right age, describes the five reading phases in children along with interest and the material to satiate the need, explains how four deterministic factors affect the reading habit of children, enlists motivations that are behind the reading process with tips to improve reading habit of children.

  13. What Is Reading?

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Reading is enjoying, entertaining and enlightening.Reading is listening, speaking and writing.Reading is talking and discussing,with yourself, with the author and with the others.Reading is exploring, investigating and guessing.Reading is travelling backward and forward, historically and geographically.

  14. STRESS ANALYSIS OF BICYCLE FRAME

    Directory of Open Access Journals (Sweden)

    Mr.M.V.Pazare

    2014-06-01

    Full Text Available This paper deals with the stress analysis of bicycle frame by using Finite Element Method. The analysis is carried out in Ansys, The F.E.A. results are compared with theoretical results. In theoretical analysis the frame is treated as truss like structure and the stresses in various members of frame like top tube, down tube, seat tube, chain stay and seat stay are determined, considering various condition like, static start up, steady state paddling, vertical impact, horizontal impact, rear wheel braking. Also Finite Element Analysis is done considering the above conditions. From the analysis it is found that there is a good agreement between analytical and F.E.A. results. Result of all the cases reveals that maximum stress is found in top tube of the bicycle frame as compared to other frame members and is equal to 24.84 MPa which is less than yield strength in tension (i.e.Syt = 290 MPa for the material (aluminum T 6061 selected.

  15. Approach/Avoidance Motivation, Message Framing, and Health Behavior: Understanding the Congruency Effect*

    OpenAIRE

    Sherman, David K.; Mann, Traci; Updegraff, John A.

    2006-01-01

    Health messages framed to be congruent with individuals' approach/avoidance motivations have been found to be more effective in promoting health behaviors than health messages incongruent with approach/avoidance motivations. This study examines the processes underlying this congruency effect. Participants (undergraduate students, N = 67) completed a measure of approach/avoidance orientation (the BIS/BAS scales) and read either a gain- or loss-framed message promoting dental flossing. Results ...

  16. The Kepler Full Frame Images

    Science.gov (United States)

    Dotson, Jessie L.; Batalha, Natalie; Bryson, Stephen T.; Caldwell, Douglas A.; Clarke, Bruce D.

    2010-01-01

    NASA's exoplanet discovery mission Kepler provides uninterrupted 1-min and 30-min optical photometry of a 100 square degree field over a 3.5 yr nominal mission. Downlink bandwidth is filled at these short cadences by selecting only detector pixels specific to 105 preselected stellar targets. The majority of the Kepler field, comprising 4 x 10(exp 6) m_v frame image. The Full Frame Images (FFIs) are calibrated by the Science Operations Center at NASA Ames Research Center. The Kepler Team employ these images for astrometric and photometric reference but make the images available to the astrophysics community through the Multimission Archive at STScI (MAST). The full-frame images provide a resource for potential Kepler Guest Observers to select targets and plan observing proposals, while also providing a freely-available long-cadence legacy of photometric variation across a swathe of the Galactic disk.

  17. Toddler Reading Time

    Science.gov (United States)

    ... KidsHealth in the Classroom What Other Parents Are Reading Upsetting News Reports? What to Say Vaccines: Which ... 5 Things to Know About Zika & Pregnancy Toddler Reading Time KidsHealth > For Parents > Toddler Reading Time Print ...

  18. Developmental reading disorder

    Science.gov (United States)

    ... page: //medlineplus.gov/ency/article/001406.htm Developmental reading disorder To use the sharing features on this page, please enable JavaScript. Developmental reading disorder is a reading disability that occurs when ...

  19. Promoting Reading Motivation by Reading Together

    Science.gov (United States)

    Monteiro, Vera

    2013-01-01

    In the present project we tested the hypothesis that tutorial situations with peers would benefit children's reading motivation. Participants were from elementary school--80 fourth-graders and 80 second-graders. We used a questionnaire to assess reading motivation. In the tutorial sessions we developed a Paired Reading Program. The children who…

  20. Cosmetology Reading Strategies. 1980 Vocational Reading Series.

    Science.gov (United States)

    Thornton, L. Jay; And Others

    Cosmetology Reading Strategies is one of five instructional guides in the Reading Strategies in Vocational Education Series. Developed to assist teachers working with students considered disadvantaged because of reading deficiency, the guide contains several strategies, suitable for adaptation, specifically related to cosmetology instruction. Each…

  1. Differential equations and moving frames

    OpenAIRE

    Abib, Odinette Renée

    2006-01-01

    The purpose of the paper is to study the relationship between differential equations, Pfaffian systems and geometric structures, via the method of moving frames of E.Cartan. We show a local structure theorem. The Lie algebra aspects differential equations is studied too.

  2. Frame Rate and Human Vision

    Science.gov (United States)

    Watson, Andrew B.

    2012-01-01

    To enhance the quality of the theatre experience, the film industry is interested in achieving higher frame rates for capture and display. In this talk I will describe the basic spatio-temporal sensitivities of human vision, and how they respond to the time sequence of static images that is fundamental to cinematic presentation.

  3. Space-Frame Lunar Lander

    Science.gov (United States)

    Curtis, Steven A.

    2010-01-01

    The space-frame lunar lander was originally intended to (1) land on rough lunar terrain, (2) deform itself to conform to the terrain so as to be able to remain there in a stable position and orientation, and (3) if required, further deform itself to perform various functions. In principle, the space-frame lunar lander could be used in the same way on Earth, as might be required, for example, to place meteorological sensors or a radio-communication relay station on an otherwise inaccessible mountain peak. the space-frame lunar lander would include a truss-like structure consisting mostly of a tetrahedral mesh of nodes connected by variable-length struts, the lengths of which would be altered in coordination to impart the desired overall size and shape to the structure. Thrusters (that is, small rocket engines), propellant tanks, a control system, and instrumentation would be mounted in and on the structure (see figure). Once it had landed and deformed itself to the terrain through coordinated variations in the lengths of the struts, the structure could be further deformed into another space-frame structure

  4. On the duality of fusion frames

    Science.gov (United States)

    Gavruta, P.

    2007-09-01

    The fusion frames were considered recently by P.G. Casazza, G. Kutyniok and S. Li in connection with distributed processing and are related to the construction of global frames from local frames. In this paper we give new results on the duality of fusion frames in Hilbert spaces.

  5. Some equalities and inequalities for fusion frames

    OpenAIRE

    Guo, Qianping; Leng, Jinsong; Li, Houbiao

    2016-01-01

    Fusion frames have some properties similar to those of frames in Hilbert spaces, but not all of their properties are similar. Some authors have established some equalities and inequalities for conventional frames. In this paper, we give some equalities and inequalities for fusion frames. Our results generalize and improve the remarkable results which have been obtained by Balan, Casazza and Gǎvruta etc.

  6. 49 CFR 393.201 - Frames.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Frames. 393.201 Section 393.201 Transportation... SAFE OPERATION Frames, Cab and Body Components, Wheels, Steering, and Suspension Systems § 393.201 Frames. (a) The frame or chassis of each commercial motor vehicle shall not be cracked, loose, sagging...

  7. Learning with sublexical information from emerging reading vocabularies in exceptionally early and normal reading development.

    Science.gov (United States)

    Thompson, G Brian; Fletcher-Flinn, Claire M; Wilson, Kathryn J; McKay, Michael F; Margrain, Valerie G

    2015-03-01

    Predictions from theories of the processes of word reading acquisition have rarely been tested against evidence from exceptionally early readers. The theories of Ehri, Share, and Byrne, and an alternative, Knowledge Sources theory, were so tested. The former three theories postulate that full development of context-free letter sounds and awareness of phonemes are required for normal acquisition, while the claim of the alternative is that with or without such, children can use sublexical information from their emerging reading vocabularies to acquire word reading. Results from two independent samples of children aged 3-5, and 5 years, with mean word reading levels of 7 and 9 years respectively, showed underdevelopment of their context-free letter sounds and phoneme awareness, relative to their word reading levels and normal comparison samples. Despite such underdevelopment, these exceptional readers engaged in a form of phonological recoding that enabled pseudoword reading, at the level of older-age normal controls matched on word reading level. Moreover, in the 5-year-old sample further experiments showed that, relative to normal controls, they had a bias toward use of sublexical information from their reading vocabularies for phonological recoding of heterophonic pseudowords with irregular consistent spelling, and were superior in accessing word meanings independently of phonology, although only if the readers were without exposure to explicit phonics. The three theories were less satisfactory than the alternative theory in accounting for the learning of the exceptionally early readers. PMID:25498743

  8. Stability of (p, Y)-Operator Frames

    Institute of Scientific and Technical Information of China (English)

    Zhi Hua GUO; Huai Xin CAO; Jun Cheng YIN

    2011-01-01

    In this paper we study the stability of (p, Y)-operator frames. We firstly discuss the relations between p-Bessel sequences (or p-frames) and (p, Y)-operator Bessel sequences (or (p, Y)-operator frames). Through defining a new union, we prove that adding some elements to a given (p, Y)-operator frame, the resulted sequence will be still a (p, Y)-operator frame. We obtain a necessary and sufficient condition for a sequence of compound operators to be a (p, Y)operator frame. Lastly, we show that (p, Y)-operator frames for X are stable under some small perturbations.

  9. Astrophysics of Reference Frame Tie Objects

    Science.gov (United States)

    Johnston, Kenneth J.; Boboltz, David; Fey, Alan Lee; Gaume, Ralph A.; Zacharias, Norbert

    2004-01-01

    The Astrophysics of Reference Frame Tie Objects Key Science program will investigate the underlying physics of SIM grid objects. Extragalactic objects in the SIM grid will be used to tie the SIM reference frame to the quasi-inertial reference frame defined by extragalactic objects and to remove any residual frame rotation with respect to the extragalactic frame. The current realization of the extragalactic frame is the International Celestial Reference Frame (ICRF). The ICRF is defined by the radio positions of 212 extragalactic objects and is the IAU sanctioned fundamental astronomical reference frame. This key project will advance our knowledge of the physics of the objects which will make up the SIM grid, such as quasars and chromospherically active stars, and relates directly to the stability of the SIM reference frame. The following questions concerning the physics of reference frame tie objects will be investigated.

  10. Redundancy of Fusion frames in Hilbert Spaces

    OpenAIRE

    Rahimi , Asghar; Zandi, Golaleh; Daraby, Bayaz

    2015-01-01

    Upon improving and extending the concept of redundancy of frames, we introduce the notion of redundancy of fusion frames, which is concerned with the properties of lower and upper redundancies. These properties are achieved by considering the minimum and maximum values of the redundancy function which is defined from the unit sphere of the Hilbert space into the positive real numbers. In addition, we study the relationship between redundancy of frames (fusion frames) and dual frames (dual fus...

  11. Nonstationary Gabor Frames - Existence and Construction

    OpenAIRE

    Dörfler, Monika; Matusiak, Ewa

    2011-01-01

    Nonstationary Gabor frames were recently introduced in adaptive signal analysis. They represent a natural generalization of classical Gabor frames by allowing for adaptivity of windows and lattice in either time or frequency. In this paper we show a general existence result for this family of frames. We then give a perturbation result for nonstationary Gabor frames and construct nonstationary Gabor frames with non-compactly supported windows from a related painless nonorthogonal expansion. Fi...

  12. Approximate duals and nearly Parseval frames

    OpenAIRE

    AZANDARYANI, MORTEZA MIRZAEE

    2015-01-01

    In this paper we introduce approximate duality of g-frames in Hilbert $C^\\ast$-modules and we show that approximate duals of g-frames in Hilbert $C^\\ast$-modules share many useful properties with those in Hilbert spaces. Moreover, we obtain some new results for approximate duality of frames and g-frames in Hilbert spaces; in particular, we consider approximate duals of $\\varepsilon$-nearly Parseval and $\\varepsilon$-close frames.

  13. Using Speed Reading and Extensive Reading Activities to Improve Students’ Reading Fluency

    OpenAIRE

    Sri Wardani

    2015-01-01

    Abstract: This study examines the implementation of Speed Reading and Extensive Reading activities to improve students’ reading fluency of students. Using a Classroom Action Research, Speed Reading and Extensive Reading activities was applied in 2 cycles with 2 x 45 minutes per week. Speed Reading and Extensive Reading activities were taught using three phase techniques: Pre-Reading, Whilst-Reading, and Post-Reading. Speed Reading was implemented through some techniques including scanning, sk...

  14. Reading Assessment: Looking Ahead

    Science.gov (United States)

    Afflerbach, Peter

    2016-01-01

    In this article, I focus on three areas of reading assessment that I believe to be crucial for students' reading development: developing comprehensive formative assessments, assessing the wide array of factors that contribute to students' reading development, and fostering student independence by helping students learn to use reading assessment on…

  15. What Should Students Read?

    Science.gov (United States)

    Wolk, Steven

    2010-01-01

    The reading students are required to do for school bears little semblance to the reading they do outside of school. Students today are reading the same texts in school that students read a generation ago, but the varieties of text used outside of school are much different. One result is that many students are unmotivated to become readers.…

  16. On Efficient Reading

    Institute of Scientific and Technical Information of China (English)

    陈伟平

    2003-01-01

    Time is limited for each reader,but many readers waste a lot oftime on unimportant things, and they read everything at the same speed and in the same way. As a result, they often fail to understand the word and the sentence; they don't know how one sentence relates to another, and how the whole text fixes together. They are not reading efficiently. It is high time that we held a discussion on efficient reading. The author states that efficient reading involves adequate comprehension with appropriate reading rate. Pointing out the factors that influence reading rate and comprehension, this article put forward some suggestions on efficient reading.

  17. G-frames and G-Riesz Bases

    OpenAIRE

    Sun, Wenchang

    2005-01-01

    G-frames are generalized frames which include ordinary frames, bounded invertible linear operators, as well as many recent generalizations of frames, e.g., bounded quasi-projectors and frames of subspaces. G-frames are natural generalizations of frames and provide more choices on analyzing functions from frame expansion coefficients. We give characterizations of g-frames and prove that g-frames share many useful properties with frames. We also give generalized version of Riesz bases and ortho...

  18. Preservation of memory-based automaticity in reading for older adults.

    Science.gov (United States)

    Rawson, Katherine A; Touron, Dayna R

    2015-12-01

    Concerning age-related effects on cognitive skill acquisition, the modal finding is that older adults do not benefit from practice to the same extent as younger adults in tasks that afford a shift from slower algorithmic processing to faster memory-based processing. In contrast, Rawson and Touron (2009) demonstrated a relatively rapid shift to memory-based processing in the context of a reading task. The current research extended beyond this initial study to provide more definitive evidence for relative preservation of memory-based automaticity in reading tasks for older adults. Younger and older adults read short stories containing unfamiliar noun phrases (e.g., skunk mud) followed by disambiguating information indicating the combination's meaning (either the normatively dominant meaning or an alternative subordinate meaning). Stories were repeated across practice blocks, and then the noun phrases were presented in novel sentence frames in a transfer task. Both age groups shifted from computation to retrieval after relatively few practice trials (as evidenced by convergence of reading times for dominant and subordinate items). Most important, both age groups showed strong evidence for memory-based processing of the noun phrases in the transfer task. In contrast, older adults showed minimal shifting to retrieval in an alphabet arithmetic task, indicating that the preservation of memory-based automaticity in reading was task-specific. Discussion focuses on important implications for theories of memory-based automaticity in general and for specific theoretical accounts of age effects on memory-based automaticity, as well as fruitful directions for future research. PMID:26302027

  19. Variance based OFDM frame synchronization

    Directory of Open Access Journals (Sweden)

    Z. Fedra

    2012-04-01

    Full Text Available The paper deals with a new frame synchronization scheme for OFDM systems and calculates the complexity of this scheme. The scheme is based on the computing of the detection window variance. The variance is computed in two delayed times, so a modified Early-Late loop is used for the frame position detection. The proposed algorithm deals with different variants of OFDM parameters including guard interval, cyclic prefix, and has good properties regarding the choice of the algorithm's parameters since the parameters may be chosen within a wide range without having a high influence on system performance. The verification of the proposed algorithm functionality has been performed on a development environment using universal software radio peripheral (USRP hardware.

  20. Alternative Genres of IS Research

    DEFF Research Database (Denmark)

    Avital, Michel; Mathiassen, Lars; Crowston, Kevin;

    2012-01-01

    perspectives, research methods, semantic framing, literary styles, and media of expression. Overall, the application of alternative genres is considered to be a generative act that provides an opportunity to take a fresh look and to gain deeper understanding of the subject matter. Nonetheless, paradoxically......The potential value of alternative genres in IS research is the core question that drives this panel discussion. The term “Alternative Genres” refers to unconventional forms of thinking, doing, and communicating scholarship and practice. It relates to innovation with respect to epistemological...... the prevailing genres have much inertia, in spite of the inherent boundary spanning nature of alternative genres. Building on their own recent empirical research, the panelists will debate the potential value of alternative genres as unconventional modality of IS scholarship and how alternative genres...

  1. Seismic performance of timber frames

    Czech Academy of Sciences Publication Activity Database

    Pospíšil, Stanislav; Urushadze, Shota; Koleš, Jan

    Prague: Czech Technical University in Prague, Faculty of Transportation Sciences, 2012 - (Jiroušek, O.; Kytýř, D.), s. 75-78 ISBN 978-80-01-05062-0. [Bilateral Czech/German Symposium, Experimental methods and numerical simulation in engineering sciences/13./. Telč (CZ), 05.06.2012-08.06.2012] Institutional support: RVO:68378297 Keywords : timber frames * dynamic test * shake table experiments Subject RIV: JM - Building Engineering

  2. Optimisation of framed child restraints

    OpenAIRE

    Dorn, Mark Richard

    1994-01-01

    This thesis documents a study into the effects of various parameters on the performance of Framed Child Seats (FCS) for automobiles. The work investigated the effect of three different sets of parameters: FCS design parameters, vehicle design parameters and occupant biomechanical parameters. The work was conducted at Middlesex University using a combination of experimental crash testing and computerised crash simulations. The experimental crash tests were conducted using the Road Safety E...

  3. Reading, Literacy, and Education

    OpenAIRE

    Lehtonen, Mikko

    2013-01-01

    In his article "Reading, Literacy, and Education" Mikko Lehtonen outlines a contextual approach to literacy. He asks how the changing relations of culture and economy, transformation of nation states and national cultures and changing notions concerning affect and cognition, transform notions of literacy and reading. Relying on the results of a recent Finnish research project on new reading communities and new ways of reading, Lehtonen highlights substantial continuities in the reading habits...

  4. The Kepler Full Frame Images

    Science.gov (United States)

    Dotson, Jessie L.; Batalha, Natalie; Bryson, Stephen T.; Caldwell, Douglas A.; Clarke, Bruce D.

    2010-01-01

    NASA's exoplanet discovery mission Kepler provides uninterrupted 1-min and 30-min optical photometry of a 100 square degree field over a 3.5 yr nominal mission. Downlink bandwidth is filled at these short cadences by selecting only detector pixels specific to 105 preselected stellar targets. The majority of the Kepler field, comprising 4 x 10(exp 6) m_v < 20 sources, is sampled at much lower 1-month cadence in the form of a full-frame image. The Full Frame Images (FFIs) are calibrated by the Science Operations Center at NASA Ames Research Center. The Kepler Team employ these images for astrometric and photometric reference but make the images available to the astrophysics community through the Multimission Archive at STScI (MAST). The full-frame images provide a resource for potential Kepler Guest Observers to select targets and plan observing proposals, while also providing a freely-available long-cadence legacy of photometric variation across a swathe of the Galactic disk.

  5. Relevance of context and time-frame in bursty dynamics

    CERN Document Server

    Jo, Hang-Hyun; Perotti, Juan I; Kaski, Kimmo

    2013-01-01

    Inhomogeneous temporal processes in natural and social phenomena have been described by bursts that are rapidly occurring events within short periods alternating with long periods of low activity. Such a temporal process can be decomposed into sub-processes, according to the contexts, i.e. circumstances in which the events occur. Then contextual bursts for each sub-process are related to context-blind bursts for the original process. This requires to study contextual bursts in real time-frame as well as in ordinal time-frame, where the real timings of events are replaced by their orders in the event sequence. By analyzing a model of uncorrelated inter-event times we find that contextual bursts in real time-frame can be dominated by either context-blind bursts or contextual bursts in ordinal time-frame, or be characterized by both. These results on the relevance of context and time-frame give insight into the origin of bursts.

  6. The Influence of Negative Advertising Frames on Political Cynicism and Politician Accountability.

    Science.gov (United States)

    Schenck-Hamlin, William J.; Procter, David E.; Rumsey, Deborah J.

    2000-01-01

    Examines the influence of negative political advertising frames on the thoughts and feelings undergraduate students generate in response to campaign advertising. Finds that participants were more likely to generate cynical comments and hold politicians accountable for the country's ills when reading candidate theme advertisements than ad hoc issue…

  7. Sparsity and spectral properties of dual frames

    DEFF Research Database (Denmark)

    Krahmer, Felix; Kutyniok, Gitta; Lemvig, Jakob

    2013-01-01

    We study sparsity and spectral properties of dual frames of a given finite frame. We show that any finite frame has a dual with no more than $n^2$ non-vanishing entries, where $n$ denotes the ambient dimension, and that for most frames no sparser dual is possible. Moreover, we derive an expression...... for the exact sparsity level of the sparsest dual for any given finite frame using a generalized notion of spark. We then study the spectral properties of dual frames in terms of singular values of the synthesis operator. We provide a complete characterization for which spectral patterns of dual...

  8. Perturbation of Wavelet and Gabor Frames

    Institute of Scientific and Technical Information of China (English)

    Ivana Carrizo; Sergio Favier

    2003-01-01

    In this work two aspects of theory of frames are presented: a side necessary condition on irregular wavelet frames is obtained, another perturbation of wavelet and Gabor frames is considered. Specifically,we present the results obtained on frame stability when one disturbs the mother of wavelet frame, or the parameter of dilatation, and in Gabor frames when the generating function or the parameter of translation are perturbed. In all cases we work without demanding compactness of the support, neither on the generating function, nor on its Fourier transform.

  9. Spacetime transformations from a uniformly accelerated frame

    International Nuclear Information System (INIS)

    We use the generalized Fermi–Walker transport to construct a one-parameter family of inertial frames which are instantaneously comoving to a uniformly accelerated observer. We explain the connection between our approach and that of Mashhoon. We show that our solutions of uniformly accelerated motion have constant acceleration in the comoving frame. Assuming the weak hypothesis of locality, we obtain local spacetime transformations from a uniformly accelerated frame K′ to an inertial frame K. The spacetime transformations between two uniformly accelerated frames with the same acceleration are Lorentz. We compute the metric at an arbitrary point of a uniformly accelerated frame. (paper)

  10. Monolithic LTCC seal frame and lid

    Energy Technology Data Exchange (ETDEWEB)

    Krueger, Daniel S.; Peterson, Kenneth A.; Stockdale, Dave; Duncan, James Brent; Riggs, Bristen

    2016-06-21

    A method for forming a monolithic seal frame and lid for use with a substrate and electronic circuitry comprises the steps of forming a mandrel from a ceramic and glass based material, forming a seal frame and lid block from a ceramic and glass based material, creating a seal frame and lid by forming a compartment and a plurality of sidewalls in the seal frame and lid block, placing the seal frame and lid on the mandrel such that the mandrel fits within the compartment, and cofiring the seal frame and lid block.

  11. Some properties of operator-valued frames

    OpenAIRE

    Gavruta, L.; Gavruta, P.

    2015-01-01

    Operator-valued frames (or g-frames) are generalizations of frames and fusion frames and have been used in packets encoding, quantum computing, theory of coherent states and more. In this paper, we give a new formula for operator-valued frames for finite dimensional Hilbert spaces. As an application, we derive in a simple manner a recent result of A. Najati concerning the approximation of g-frames by Parseval ones. We obtain also some results concerning the best approximation of operator-valu...

  12. Renewable Energy Alternatives in Maryland.

    Science.gov (United States)

    Welsh, Greg E.; McClellan, Deborah A. S.

    This handbook discusses the renewable energy resources suitable for use in Maryland. It follows a question and answer format with sections about the following alternative renewable energy sources; solar, wind, wood, water, bio-gas/methane, and geothermal. Each section includes a list of recommended readings, appropriate agencies or organizations,…

  13. Mars Science Laboratory Frame Manager for Centralized Frame Tree Database and Target Pointing

    Science.gov (United States)

    Kim, Won S.; Leger, Chris; Peters, Stephen; Carsten, Joseph; Diaz-Calderon, Antonio

    2013-01-01

    The FM (Frame Manager) flight software module is responsible for maintaining the frame tree database containing coordinate transforms between frames. The frame tree is a proper tree structure of directed links, consisting of surface and rover subtrees. Actual frame transforms are updated by their owner. FM updates site and saved frames for the surface tree. As the rover drives to a new area, a new site frame with an incremented site index can be created. Several clients including ARM and RSM (Remote Sensing Mast) update their related rover frames that they own. Through the onboard centralized FM frame tree database, client modules can query transforms between any two frames. Important applications include target image pointing for RSM-mounted cameras and frame-referenced arm moves. The use of frame tree eliminates cumbersome, error-prone calculations of coordinate entries for commands and thus simplifies flight operations significantly.

  14. To read or not to read

    OpenAIRE

    Mol, Suzanne Elizabeth

    2010-01-01

    There is a widely held belief that reading (story)books makes us smarter and helps promote success in life. Does scientific evidence support this notion? The three meta-analyses in this thesis comprise 146 studies between 1988 and 2010 (N=10,308 participants) that addressed the role of book reading in language and reading development from infancy to early adulthood. Chapter 2 shows that reading routines that are part of children’s and students’ leisure-time activities offer substantial advant...

  15. Frames of exponentials:lower frame bounds for finite subfamilies, and approximation of the inverse frame operator

    DEFF Research Database (Denmark)

    Christensen, Ole; Lindner, Alexander M

    We give lower frame bounds for finite subfamilies of a frame of exponentials {e(i lambdak(.))}k is an element ofZ in L-2(-pi,pi). We also present a method for approximation of the inverse frame operator corresponding to {e(i lambdak(.))}k is an element ofZ, where knowledge of the frame bounds for...... finite subfamilies is crucial. (C) 2001 Elsevier Science Inc. All rights reserved....

  16. What Oral Text Reading Fluency Can Reveal about Reading Comprehension

    Science.gov (United States)

    Veenendaal, Nathalie J.; Groen, Margriet A.; Verhoeven, Ludo

    2015-01-01

    Text reading fluency--the ability to read quickly, accurately and with a natural intonation--has been proposed as a predictor of reading comprehension. In the current study, we examined the role of oral text reading fluency, defined as text reading rate and text reading prosody, as a contributor to reading comprehension outcomes in addition to…

  17. Reading program-Remedial, integrated, and innovative.

    Science.gov (United States)

    Butler, S R

    1991-01-01

    An innovative integrated remedial reading program has been developed based on recent research findings. My longitudinal studies have revealed that poor reading compounds itself over the years. The majority of children with reading disabilities currently remain in regular classrooms with varying techniques being used depending upon individual school directives and current educational theory.Despite current remedial techniques, the poorer reader tends to remain so throughout the school years. Innovative techniques must be developed in the hope of altering this pattern.This paper presents one alternative strategy which can be used to upgrade reading skills and break the cycle of reading failure. The Reading Assistance Tutorial Pack (R.A.T. Pack) is a carefully sequenced series of activities that enables the learner to experience the motivating and reinforcing properties of success through all stages of phonetic and reading skills development.It is a systematic, multidisciplinary remedial reading program based on sound behavior, psycholinguistic and cognitive theories of learning-incorporating listening, speaking, seeing, writing, thinking, and comprehension skills. The R.A.T. Pack demands a high percentage of on-task behavior and trains phonological processing strategies. Functional language use is promoted through enjoyable activities involving sentence construction, cloze passages, puzzles, games, and other creative manipulations of the surface features of languages. The program has proven successful in schools, homes, and clinics. PMID:24233760

  18. FUSION FRAMES AND G-FRAMES IN TENSOR PRODUCT AND DIRECT SUM OF HILBERT SPACES

    OpenAIRE

    M. Mirzaee Azandaryani; Amir Khosravi

    2012-01-01

    In this paper we study fusion frames and g-frames for the tensor products and direct sums of Hilbert spaces. We show that the tensor product of a finite number of g-frames (resp. fusion frames, g-Riesz bases) is a g-frame (resp. fusion frame, g-Riesz basis) for the tensor product space and vice versa. Moreover we obtain some important results in tensor products and direct sums of g-frames, fusion frames, resolutions of the identity and duals.

  19. A fundamental identity for Parseval frames

    OpenAIRE

    Balan, R.; Casazza, P. G.; Edidin, D; Kutyniok, G.

    2005-01-01

    In this paper we establish a suprising fundamental identity for Parseval frames in a Hilbert space. Several variations of this result are given, including an extension to general frames. Finally, we discuss the derived results.

  20. Cultural Factors in Reading

    Institute of Scientific and Technical Information of China (English)

    孔敏

    2005-01-01

    Reading is a basic ability in learning English and reading comprehension exercise is a common way to assess this ability.Since reading is a communicative activity between author and reader in written form,there are some different rules and regulations of this communication in different countries.Therefore,cultural factors,existing in reading,decide,help,and influence the percentage of the right answers.This article attempts to analyze the effects of cultural differences in reading and the barriers in comprehension,and aims to improve students awareness of cultural differences in reading.

  1. Rest frame of bubble nucleation

    Energy Technology Data Exchange (ETDEWEB)

    Garriga, Jaume [Departament de Física Fondamental i Institut de Ciencies del Cosmos, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona (Spain); Kanno, Sugumi [Institute of Cosmology, Department of Physics and Astronomy, Tufts University, Medford, Massachusetts 02155 (United States); Tanaka, Takahiro, E-mail: jaume.garriga@ub.edu, E-mail: sugumi@cosmos.phy.tufts.edu, E-mail: tanaka@yukawa.kyoto-u.ac.jp [Yukawa Institute for Theoretical Physics, Kyoto University, Kyoto 606-8502 (Japan)

    2013-06-01

    Vacuum bubbles nucleate at rest with a certain critical size and subsequently expand. But what selects the rest frame of nucleation? This question has been recently addressed in [1] in the context of Schwinger pair production in 1+1 dimensions, by using a model detector in order to probe the nucleated pairs. The analysis in [1] showed that, for a constant external electric field, the adiabatic ''in'' vacuum of charged particles is Lorentz invariant, (and in this) case pairs tend to nucleate preferentially at rest with respect to the detector. Here, we sharpen this picture by showing that the typical relative velocity between the frame of nucleation and that of the detector is at most of order Δv ∼ S{sub E}{sup −1/3} << 1. Here, S{sub E} >> 1 is the action of the instanton describing pair creation. The bound Δv coincides with the minimum uncertainty in the velocity of a non-relativistic charged particle embedded in a constant electric field. A velocity of order Δv is reached after a time interval of order Δt ∼ S{sub E}{sup −1/3}r{sub 0} << r{sub 0} past the turning point in the semiclassical trajectory, where r{sub 0} is the size of the instanton. If the interaction takes place in the vicinity of the turning point, the semiclassical description of collision does not apply. Nonetheless, we find that even in this case there is still a strong asymmetry in the momentum transferred from the nucleated particles to the detector, in the direction of expansion after the turning point. We conclude that the correlation between the rest frame of nucleation and that of the detector is exceedingly sharp.

  2. Quantum games in noninertial frame

    CERN Document Server

    Khan, Salman

    2011-01-01

    We study the influence of Unruh effect on quantum non-zero sum games. In particular, we investigate the quantum prisoners' dilemma under different conditions and show that the acceleration of the noninertial frames disturbs the symmetry of the game. It is shown that under some particular situations, the classical strategy C (cooperation) becomes the dominant strategy. Our investigation shows that any quantum strategy does no better for any player against the classical strategies. The miracle move of Eisert et al [5] is no more a superior move. We show that the dilemma like situation under every condition of the game is resolved.

  3. Some equalities and inequalities for fusion frames.

    Science.gov (United States)

    Guo, Qianping; Leng, Jinsong; Li, Houbiao

    2016-01-01

    Fusion frames have some properties similar to those of frames in Hilbert spaces, but not all of their properties are similar. Some authors have established some equalities and inequalities for conventional frames. In this paper, we give some equalities and inequalities for fusion frames. Our results generalize and improve the remarkable results which have been obtained by Balan, Casazza and Gǎvruta etc. PMID:26904390

  4. The fundamentals of Spectral Tetris frame constructions

    OpenAIRE

    Casazza, Peter G.; Woodland, Lindsey M.

    2014-01-01

    The landmark paper "Constructing tight fusion frames" by Casazza, Fickus, Mixon, Wang and Zhou introduced a fundamental method for constructing unit norm tight frames, which they called Spectral Tetris. This was a significant advancement for finite frame theory - especially constructions of finite frames. This paper then generated a vast amount of literature as Spectral Tetris was steadily developed, refined, and generalized until today we have a complete picture of what are the broad applica...

  5. Tight and random nonorthogonal fusion frames

    OpenAIRE

    Cahill, Jameson; Casazza, Peter G.; Ehler, Martin; Li, Shidong

    2013-01-01

    First we show that tight nonorthogonal fusion frames a relatively easy to com by. In order to do this we need to establish a classification of how to to wire a self adjoint operator as a product of (nonorthogonal) projection operators. We also discuss the link between nonorthogonal fusion frames and positive operator valued measures, we define and study a nonorthogonal fusion frame potential, and we introduce the idea of random nonorthogonal fusion frames.

  6. Framing Biotechnology in Iranian TV Series

    OpenAIRE

    H. Khaniki; M. H. Panahi; M. A. Ghaneirad; Z. Zardar

    2014-01-01

    Media as public opinion formers have crucial role in supporting the growth and development of science and technology . They form media frames in various fields of science and Technology. This paper seeks to identify frames which Iranian television series depict biotechnology. The Biotechnology frames Identified through qualitative framing analysis. To achieve this goal, all TV series of Five main national channels for a five-year period (2008-2013) were considered and two TV series – “Balhaye...

  7. Systematic DFT Frames: Principle and Eigenvalues Structure

    OpenAIRE

    Vaezi, Mojtaba; Labeau, Fabrice

    2012-01-01

    Motivated by a host of recent applications requiring some amount of redundancy, frames are becoming a standard tool in the signal processing toolbox. In this paper, we study a specific class of frames, known as discrete Fourier transform (DFT) codes, and introduce the notion of systematic frames for this class. This is encouraged by application of systematic DFT codes in distributed source coding using DFT codes, a new application for frames. Studying their extreme eigenvalues, we show that, ...

  8. Deletion of Dystrophin In-Frame Exon 5 Leads to a Severe Phenotype: Guidance for Exon Skipping Strategies.

    Science.gov (United States)

    Toh, Zhi Yon Charles; Thandar Aung-Htut, May; Pinniger, Gavin; Adams, Abbie M; Krishnaswarmy, Sudarsan; Wong, Brenda L; Fletcher, Sue; Wilton, Steve D

    2016-01-01

    Duchenne and Becker muscular dystrophy severity depends upon the nature and location of the DMD gene lesion and generally correlates with the dystrophin open reading frame. However, there are striking exceptions where an in-frame genomic deletion leads to severe pathology or protein-truncating mutations (nonsense or frame-shifting indels) manifest as mild disease. Exceptions to the dystrophin reading frame rule are usually resolved after molecular diagnosis on muscle RNA. We report a moderate/severe Becker muscular dystrophy patient with an in-frame genomic deletion of DMD exon 5. This mutation has been reported by others as resulting in Duchenne or Intermediate muscular dystrophy, and the loss of this in-frame exon in one patient led to multiple splicing events, including omission of exon 6, that disrupts the open reading frame and is consistent with a severe phenotype. The patient described has a deletion of dystrophin exon 5 that does not compromise recognition of exon 6, and although the deletion does not disrupt the reading frame, his clinical presentation is more severe than would be expected for classical Becker muscular dystrophy. We suggest that the dystrophin isoform lacking the actin-binding sequence encoded by exon 5 is compromised, reflected by the phenotype resulting from induction of this dystrophin isoform in mouse muscle in vivo. Hence, exon skipping to address DMD-causing mutations within DMD exon 5 may not yield an isoform that confers marked clinical benefit. Additional studies will be required to determine whether multi-exon skipping strategies could yield more functional dystrophin isoforms, since some BMD patients with larger in-frame deletions in this region have been reported with mild phenotypes. PMID:26745801

  9. Deletion of Dystrophin In-Frame Exon 5 Leads to a Severe Phenotype: Guidance for Exon Skipping Strategies.

    Directory of Open Access Journals (Sweden)

    Zhi Yon Charles Toh

    Full Text Available Duchenne and Becker muscular dystrophy severity depends upon the nature and location of the DMD gene lesion and generally correlates with the dystrophin open reading frame. However, there are striking exceptions where an in-frame genomic deletion leads to severe pathology or protein-truncating mutations (nonsense or frame-shifting indels manifest as mild disease. Exceptions to the dystrophin reading frame rule are usually resolved after molecular diagnosis on muscle RNA. We report a moderate/severe Becker muscular dystrophy patient with an in-frame genomic deletion of DMD exon 5. This mutation has been reported by others as resulting in Duchenne or Intermediate muscular dystrophy, and the loss of this in-frame exon in one patient led to multiple splicing events, including omission of exon 6, that disrupts the open reading frame and is consistent with a severe phenotype. The patient described has a deletion of dystrophin exon 5 that does not compromise recognition of exon 6, and although the deletion does not disrupt the reading frame, his clinical presentation is more severe than would be expected for classical Becker muscular dystrophy. We suggest that the dystrophin isoform lacking the actin-binding sequence encoded by exon 5 is compromised, reflected by the phenotype resulting from induction of this dystrophin isoform in mouse muscle in vivo. Hence, exon skipping to address DMD-causing mutations within DMD exon 5 may not yield an isoform that confers marked clinical benefit. Additional studies will be required to determine whether multi-exon skipping strategies could yield more functional dystrophin isoforms, since some BMD patients with larger in-frame deletions in this region have been reported with mild phenotypes.

  10. Beyond the reading list - inspiring wider reading

    OpenAIRE

    Bedford, David

    2015-01-01

    Presentation of activities used to broaden the reading horizons of a group of students who had been identified as not reading broadly enough. A range of active learning activities were used, but the presentation focuses on an activity using Moodle’s Wiki tool.

  11. Lights, Camera, Read! Arizona Reading Program Manual.

    Science.gov (United States)

    Arizona State Dept. of Library, Archives and Public Records, Phoenix.

    This document is the manual for the Arizona Reading Program (ARP) 2003 entitled "Lights, Camera, Read!" This theme spotlights books that were made into movies, and allows readers to appreciate favorite novels and stories that have progressed to the movie screen. The manual consists of eight sections. The Introduction includes welcome letters from…

  12. To read or not to read

    NARCIS (Netherlands)

    Mol, Suzanne Elizabeth

    2010-01-01

    There is a widely held belief that reading (story)books makes us smarter and helps promote success in life. Does scientific evidence support this notion? The three meta-analyses in this thesis comprise 146 studies between 1988 and 2010 (N=10,308 participants) that addressed the role of book reading

  13. Oversampling of wavelet frames for real dilations

    DEFF Research Database (Denmark)

    Bownik, Marcin; Lemvig, Jakob

    2012-01-01

    We generalize the Second Oversampling Theorem for wavelet frames and dual wavelet frames from the setting of integer dilations to real dilations. We also study the relationship between dilation matrix oversampling of semi-orthogonal Parseval wavelet frames and the additional shift invariance gain...

  14. Fusion Frames and the Restricted Isometry Property

    OpenAIRE

    Bodmann, Bernhard,; Cahill, Jameson; Casazza, Peter G.

    2011-01-01

    We will show that tight frames satisfying the restricted isometry property give rise to nearly tight fusion frames which are nearly orthogonal and hence are nearly equi-isoclinic. We will also show how to replace parts of the RIP frame with orthonormal sets while maintaining the RIP property.

  15. Classifying tight Weyl-Heisenberg frames

    DEFF Research Database (Denmark)

    Cazsazza, P.; Janssen, A. J. E. M.; Christensen, Ole

    1999-01-01

    A Weyl-Heisenberg frame for L^2(R) is a frame consisting of translates and modulates of a fixed function. In this paper we give necessary and sufficient conditions for this family to form a tight WH-frame. This allows us to write down explicitly all functions g for which all translates and...

  16. Influence of framing on medical decision making

    OpenAIRE

    Feng, Jun; Gong, Jingjing; Huang, Yonghua; Wei, Yazhou; Zhang, Weiwei; Zhang, Yan

    2013-01-01

    Numerous studies have demonstrated the robustness of the framing effect in a variety of contexts, especially in medical decision making. Unfortunately, research is still inconsistent as to how so many variables impact framing effects in medical decision making. Additionally, much attention should be paid to the framing effect not only in hypothetical scenarios but also in clinical experience.

  17. PARAMETRIC RESONANSE IN STATICALLY INDETERMINATE FRAMES

    Directory of Open Access Journals (Sweden)

    Yu.V. Vorona

    2014-12-01

    Full Text Available The technique for development and reduction of discrete dynamics models of frames is presented. Construction of the models is carried out using finite element method, generalized coordinates and tools of modern computer software. Parametric resonance in a statically indeterminate frame caused by external excitations is investigated. Main instability domains of the frame are determined.

  18. Parametric Study Of Window Frame Geometry

    DEFF Research Database (Denmark)

    Zajas, Jan Jakub; Heiselberg, Per

    This paper describes a parametric study on window frame geometry with the goal of designing frames with very good thermal properties. Three different parametric frame models are introduced, deseribed by a number of variables. In the first part of the study, a process of sensitivity analysis is...

  19. Non-Syntactic Antecedents and Frame Semantics.

    Science.gov (United States)

    Gensler, Orin

    A polemic is made for frame semantics and the linguistic phenomenon of anaphoric reference without noun phrase (NP) antecedent is examined within this frame. Non-syntactic anaphora is that which does not point out into the real world but rather points back into the discourse in a frame which has been built up between the speaker and hearer in a…

  20. 10 CFR 710.35 - Time frames.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Time frames. 710.35 Section 710.35 Energy DEPARTMENT OF... Matter or Special Nuclear Material Miscellaneous § 710.35 Time frames. Statements of time established for processing aspects of a case under this subpart are the agency's desired time frames in implementing...

  1. 21 CFR 886.5842 - Spectacle frame.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Spectacle frame. 886.5842 Section 886.5842 Food... DEVICES OPHTHALMIC DEVICES Therapeutic Devices § 886.5842 Spectacle frame. (a) Identification. A spectacle frame is a device made of metal or plastic intended to hold prescription spectacle lenses worn by...

  2. A Framed Temporal Logic Programming Language

    Institute of Scientific and Technical Information of China (English)

    Zhen-HuaDuan; MaciejKoutny

    2004-01-01

    We discuss the projection temporal logic (PTL), based on a primitive projection operator, prj. A framing technique is also presented, using which a synchronization operator, await, is defined within the underlying logic. A framed temporal logic programming language (FTLL) is presented. To illustrate how to use both the language and framing technique, some examples are given.

  3. Inertial reference frames and gravitational forces

    International Nuclear Information System (INIS)

    The connection between different definitions of inertial, i.e. fundamental, reference frames and the corresponding characterisation of gravitational fields by gravitational forces are considered from the point of view of their possible interpretation in university introductory courses. The introduction of a special class of reference frames, denoted 'mixed reference frames' is proposed and discussed. (author)

  4. Classifying tight Weyl-Heisenberg frames

    DEFF Research Database (Denmark)

    Cazsazza, P.; Janssen, A. J. E. M.; Christensen, Ole

    A Weyl-Heisenberg frame for L^2(R) is a frame consisting of translates and modulates of a fixed function. In this paper we give necessary and sufficient conditions for this family to form a tight WH-frame. This allows us to write down explicitly all functions g for which all translates and modula...

  5. On English Reading Skills

    Institute of Scientific and Technical Information of China (English)

    钱芬

    2008-01-01

    Reading is one of the four important skills in English learning.It is also a skill that the students need to possess to support independent and self-directed learning.With the development of society,science and technology develop at hish speed and the competition in the society become sharp.Reading is a way for students to be more knowledgeable and successful.So,it becomes more and more important to speed up their reading in order to acquire as much information as possible.Thus,fostering a good English reading habit is essential,and being able to adopt different reading skills for different reading materials and purposes will also help to read more effectively.The paper mainly concenls some basic English reading skills.

  6. What Are Reading Disorders?

    Science.gov (United States)

    ... Information Clinical Trials Resources and Publications What are reading disorders? Skip sharing on social media links Share ... for more information about these problems. Types of Reading Disorders Dyslexia is a brain-based type of ...

  7. Teaching Reading Skills

    Institute of Scientific and Technical Information of China (English)

    刘恒

    2014-01-01

    Reading skills are very important part in language teaching and learning. This paper is written after attending lectures given by an Australian teacher named Rod Ellis focusing on how to teach reading skills using authentic materials.

  8. Complementary and Alternative Therapies in ALS

    OpenAIRE

    Bedlack, Richard S.; Joyce, Nanette; Carter, Gregory T.; Pagononi, Sabrina; Karam, Chafic

    2015-01-01

    Given the severity of their illness and lack of effective disease modifying agents, it is not surprising that most patients with ALS consider trying complementary and alternative therapies. Some of the most commonly considered alternative therapies include special diets, nutritional supplements, cannabis, acupuncture, chelation and energy healing. This chapter reviews these in detail. We also describe 3 models by which physicians may frame discussions about alternative therapies: paternalism,...

  9. Reading sentences in Serbian: Effects of alphabet and reading mode in self-paced reading task

    OpenAIRE

    Vejnović Dušan; Jovanović Tamara

    2012-01-01

    The study examined the influence of alphabet (Cyrillic vs. Latin) and reading mode (silent reading vs. reading aloud) on sentence reading speed in Serbian. Entire-sentence and single-word reading times were obtained from the moving window paradigm in the self-paced sentence reading task. Sentences printed in Latin took less time for reading than sentences printed in Cyrillic and silent reading was more rapid than reading aloud. Single-word processing results followed the pattern observe...

  10. Tactics for Reading Comprehension

    Institute of Scientific and Technical Information of China (English)

    孔祥航; 张艳荣

    2003-01-01

    In recent years, reading comprehension is taking up a larger and larger part in almost every international test or domestic examination. Knowing the basic knowledge and grasping the test - taking tactics are key factors of good reading comprehension. In this thesis, I will dwell on nine commonly used tactics for reading comprehension. This will help you to deal with the problems with reading comprehension efficiently.

  11. Frames and generalized shift-invariant systems

    DEFF Research Database (Denmark)

    Christensen, Ole

    With motivation from the theory of Hilbert-Schmidt operators we review recent topics concerning frames in L 2 (R) and their duals. Frames are generalizations of orthonormal bases in Hilbert spaces. As for an orthonormal basis, a frame allows each element in the underlying Hilbert space to be...... written as an unconditionally convergent infinite linear combination of the frame elements; however, in contrast to the situation for a basis, the coefficients might not be unique. We present the basic facts from frame theory and the motivation for the fact that most recent research concentrates on tight...

  12. Incorporating RTI in a Hybrid Model of Reading Disability

    Science.gov (United States)

    Spencer, Mercedes; Wagner, Richard K.; Schatschneider, Christopher; Quinn, Jamie M.; Lopez, Danielle; Petscher, Yaacov

    2014-01-01

    The present study seeks to evaluate a hybrid model of identification that incorporates response to instruction and intervention (RTI) as one of the key symptoms of reading disability. The 1-year stability of alternative operational definitions of reading disability was examined in a large-scale sample of students who were followed longitudinally…

  13. ELLs' Perceptions of Reading

    Science.gov (United States)

    Howard, Rachael M.

    2012-01-01

    This research investigated reading support and book preferences of fourth grade English language learners (ELLs) who were struggling readers. This qualitative research focused on three case studies. Interviews were conducted to explore ELLs' perceptions on reading motivation, reading programs, and types of support they received. Descriptions of…

  14. Voiced Reading and Rhythm

    Institute of Scientific and Technical Information of China (English)

    詹艳萍

    2007-01-01

    Since voiced reading is an important way in learning English,rhythm is the most critical factor that enables to read beautifully.This article illustrates the relationship between rhythm and voiced reading,the importance of rhythm,and the methods to develop the sense of rhythm.

  15. Guided Reading and Motivation

    Science.gov (United States)

    Hauptman, Allyson L.

    2012-01-01

    The purpose of this study was to determine the relationship between Guided Reading and student motivation to read across fourth, fifth, and sixth grades. The study defined literacy motivation as: (a) task value; (b) self-perceived competence; (c) students' perceptions of the Guided Reading format. Factor analysis and repeated measures ANOVAs were…

  16. Reading Difficulties in Albanian

    Science.gov (United States)

    Avdyli, Rrezarta; Cuetos, Fernando

    2012-01-01

    Albanian is an Indo-European language with a shallow orthography, in which there is an absolute correspondence between graphemes and phonemes. We aimed to know reading strategies used by Albanian disabled children during word and pseudoword reading. A pool of 114 Kosovar reading disabled children matched with 150 normal readers aged 6 to 11 years…

  17. Free Reading Is UTOPIA

    Science.gov (United States)

    LeCrone, Nancy

    2010-01-01

    In high school students get tied up in extracurricular activities and have little time for pleasure reading. It is true that with rigorous academic schedules they have little time for pleasure reading. Thus began a conversation with a sophomore English teacher at the author's high school. As they were discussing the plight of free reading he was…

  18. The Reading Clinic.

    Science.gov (United States)

    Ridout, Susan Ramp; Bailey, Kevin Sue

    Organized to allow undergraduate and graduate students to work together in helping children learn to read, this practicum manual provides guidelines and materials needed for the undergraduate section of the Methods of Teaching Reading II Reading Practicum at Indiana University Southeast. In addition to the syllabus, which includes a course…

  19. Supervising the Reading Clinician.

    Science.gov (United States)

    Ridout, Susan Ramp; Bailey, Kevin Sue

    Designed for graduate students supervising undergraduate work in a reading clinic, this practicum manual provides guidelines and materials needed for the graduate section of the Reading Practicum (Methods of Teaching Reading II) at Indiana University Southeast. In addition to the syllabus, which includes course description and objectives, course…

  20. Literature without Reading.

    Science.gov (United States)

    Knighten, Katherine W.

    During one term at a laboratory school, reading was systematically deleted from activites in one secondary level class and replaced with film, lecture, oral reading, slides, and other audiovisual aids. The Nelson-Denny Reading Test served as pretest-posttest for the twelve-week experiment, with 19 of 25 students completing both tests. The results…

  1. Reading to Learn

    Science.gov (United States)

    Herman, Phillip; Wardrip, Peter

    2012-01-01

    Science teachers expect high school students to know how to read, understand, and learn from texts at the core of the curriculum. But though students learn to read in grade school, many do not know how to "read to learn" science. And science teachers are often too busy teaching science to actively help students increase their science reading…

  2. Dutch for Reading Knowledge

    NARCIS (Netherlands)

    C. van Baalen; F.R.E. Blom; I. Hollander

    2012-01-01

    This first Dutch for Reading Knowledge book on the market promotes a high level of reading and translation competency by drawing from Dutch grammar, vocabulary and reading strategies, and providing many translation "shortcuts" and tips when tackling complex texts in Dutch. Aimed at students, researc

  3. EFFECTS OF MESSAGE FRAMING AND EXEMPLARS ON PROMOTING ORGAN DONATION.

    Science.gov (United States)

    Chien, Yu-Hung; Chang, Wen-Te

    2015-12-01

    People in many countries are unwilling to donate organs. Drawing on previous research regarding the use of message framing and the theory of exemplification promoting intentions to donate organs, this study examined messaging strategies. This study used a 2 × 2 between-subjects factorial design to examine the joint effects of gain/loss frames and statistical/exemplar appeals on the intentions of 189 Taiwanese college students (108 women, 81 men; age range = 19-24 yr., M = 21.6, SD = 2.9) regarding organ donation. Each participant was randomly assigned to read one of four versions of an organ donation promotional message and then to complete a questionnaire. The analysis of variance showed a significant interaction between the two factors. Loss-exemplar messages elicited significantly more positive intentions toward donation than did loss-statistical messages. There was no significant difference between the statistical and exemplar appeals observed under the gain-framed condition. The practical implications of developing effective organ donation promotional materials and the limitations of this study are discussed. PMID:26595292

  4. Interpretation versus Reference Framing: Assimilation and Contrast Effects in the Organizational Domain.

    Science.gov (United States)

    Stapel; Koomen

    1998-11-01

    We examined conditions under which contextually activated information affects strategic decision-making and found that the subjective framing of organizational issues may be affected by variables other than semantic manipulation. Context information may be used as an interpretation frame (and lead to assimilation) or as a reference frame (and lead to contrast). Whether context information instigates assimilative interpretation or contrastive comparison processes may depend on the level of categorical context-target similarity. This is demonstrated in three experiments in which participants read an unambiguous business threat or opportunity scenario prior to judging an ambiguous, strategic issue. Findings are discussed in the light of previous judgment and decision-making studies of framing and context effects. Copyright 1998 Academic Press. PMID:9831519

  5. Nonlinear pushover analysis of infilled concrete frames

    Institute of Scientific and Technical Information of China (English)

    Chao Hsun Huang; Yungting Alex Tuan; Ruo Yun Hsu

    2006-01-01

    Six reinforced concrete frames with or without masonry infills were constructed and tested under horizontal cyclic loads. All six frames had identical details in which the transverse reinforcement in columns was provided by rectangular hoops that did not meet current ACI specifications for ductile frames. For comparison purposes, the columns in three of these frames were jacketed by carbon-fiber-reinforced-polymer (CFRP) sheets to avoid possible shear failure. A nonlinear pushover analysis, in which the force-deformation relationships of individual elements were developed based on ACI 318, FEMA 356,and Chen's model, was carried out for these frames and compared to test results. Both the failure mechanisms and impact of infills on the behaviors of these frames were examined in the study. Conclusions from the present analysis provide structural engineers with valuable information for evaluation and design of infilled concrete frame building structures.

  6. Understanding What Students Know: Evaluating Their Online Research and Reading Comprehension Skills

    Science.gov (United States)

    Castek, Jill; Coiro, Julie

    2015-01-01

    This piece is framed by questions we are often asked when we talk about online reading assessments and instruction with teachers. We begin with some of the lessons we have learned in our own experiences with designing measures of online reading comprehension. Then we share our thoughts about key design considerations as well as some of the biggest…

  7. How to Communicate with a Machine: On Reading a Public Library's OPAC

    Science.gov (United States)

    Saarti, Jarmo; Raivio, Jouko

    2011-01-01

    This article presents a reading of the user interface in one public library system. Its aim is to find out the frames and competences required and used in the communication between the computer and the patron. The authors see the computer as a text that is to be read by the user who wants to search for information from the library. The transition…

  8. Reading "Salt and Pepper": Social Practices, Unfinished Narratives, and Critical Interpretations

    Science.gov (United States)

    Anderson, Diane Downer

    2008-01-01

    In "Reading "Salt and Pepper"" Anderson examines a story written by three third grade girls and their insights about that story as they re-read it during its production and retrospectively, eight years later. Using a frame for understanding children's writing as social practice, the children's interviews, showing their multiple and sometimes…

  9. Decompositions of g-Frames and Duals and Pseudoduals of g-Frames in Hilbert Spaces

    OpenAIRE

    Guo, Xunxiang

    2015-01-01

    Firstly, we study the representation of g-frames in terms of linear combinations of simpler ones such as g-orthonormal bases, g-Riesz bases, and normalized tight g-frames. Then, we study the dual and pseudodual of g-frames, which are critical components in reconstructions. In particular, we characterize the dual g-frames in a constructive way; that is, the formulae for dual g-frames are given. We also give some g-frame like representations for pseudodual g-frame pairs. The operator parameteri...

  10. Reading through Linguistic Borderlands: Latino Students' Transactions with Narrative Texts

    Science.gov (United States)

    Martinez-Roldan, Carmen; Sayer, Peter

    2006-01-01

    This study examines the biliteracy development of a group of bilingual Latino third graders in an elementary school in the south-west USA. It focuses on the role of language in children's reading comprehension of narrative texts in Spanish and English in a school context. The authors frame their analysis within the "Continua of Biliteracy" model…

  11. What oral text reading fluency can reveal about reading comprehension

    NARCIS (Netherlands)

    Veenendaal, N.J.; Groen, M.A.; Verhoeven, L.T.W.

    2015-01-01

    Text reading fluency – the ability to read quickly, accurately and with a natural intonation – has been proposed as a predictor of reading comprehension. In the current study, we examined the role of oral text reading fluency, defined as text reading rate and text reading prosody, as a contributor t

  12. Do reading and spelling share a lexicon?

    Science.gov (United States)

    Jones, Angela C; Rawson, Katherine A

    2016-05-01

    In the reading and spelling literature, an ongoing debate concerns whether reading and spelling share a single orthographic lexicon or rely upon independent lexica. Available evidence tends to support a single lexicon account over an independent lexica account, but evidence is mixed and open to alternative explanation. In the current work, we propose another, largely ignored account--separate-but-shared lexica--according to which reading and spelling have separate orthographic lexica, but information can be shared between them. We report three experiments designed to competitively evaluate these three theoretical accounts. In each experiment, participants learned new words via reading training and/or spelling training. The key manipulation concerned the amount of reading versus spelling practice a given item received. Following training, we assessed both response time and accuracy on final outcome measures of reading and spelling. According to the independent lexica account, final performance in one modality will not be influenced by the level of practice in the other modality. According to the single lexicon account, final performance will depend on the overall amount of practice regardless of modality. According to the separate-but-shared account, final performance will be influenced by the level of practice in both modalities but will benefit more from same-modality practice. Results support the separate-but-shared account, indicating that reading and spelling rely upon separate lexica, but information can be shared between them. PMID:26999066

  13. Frame Based Symmetric Key Cryptography

    Directory of Open Access Journals (Sweden)

    Uttam Kr. Mondal

    2011-01-01

    Full Text Available There are huge numbers of algorithms available in symmetry key block cipher. All these algorithms have been used either complicated keys to produce cipher text from plain text or a complicated algorithms for it. The level of security of all algorithms is dependent on either number of iterations or length of keys. In this paper, a symmetry key block cipher algorithm has been proposed to encrypt plain text into cipher text or vice versa using a frame set. A comparative study have been made with RSA, DES, IDEA, BAM and other algorithms with Chi-square value, frequency distribution, bit ratio to check the security level of proposed algorithm. Finally, a comparison has been made for time complexity for encryption of plain text and decryption from cipher text with the well-known existing algorithms.

  14. Fingerprinting with Equiangular Tight Frames

    CERN Document Server

    Mixon, Dustin G; Kiyavash, Negar; Fickus, Matthew

    2011-01-01

    Digital fingerprinting is a framework for marking media files, such as images, music, or movies, with user-specific signatures to deter illegal distribution. Multiple users can collude to produce a forgery that can potentially overcome a fingerprinting system. This paper proposes an equiangular tight frame fingerprint design which is robust to such collusion attacks. We motivate this design by considering digital fingerprinting in terms of compressed sensing. The attack is modeled as linear averaging of multiple marked copies before adding a Gaussian noise vector. The content owner can then determine guilt by exploiting correlation between each user's fingerprint and the forged copy. The worst-case error probability of this detection scheme is analyzed and bounded. Simulation results demonstrate the average-case performance is similar to the performance of orthogonal and simplex fingerprint designs, while accommodating several times as many users.

  15. Hunting down frame shifts: Ecological analysis of diverse functional gene sequences

    Directory of Open Access Journals (Sweden)

    Michal eStrejcek

    2015-11-01

    Full Text Available Functional gene ecological analyses using amplicon sequencing can be challenging as translated sequences are often burdened with shifted reading frames. The aim of this work was to evaluate several bioinformatics tools designed to correct errors which arise during sequencing in an effort to reduce the number of frame-shifts (FS. Genes encoding for alpha subunits of biphenyl (bphA and benzoate (benA dioxygenases were used as model sequences. FrameBot, a FS correction tool, was able to reduce the number of detected FS to zero. However, up to 43.1% of sequences were discarded by FrameBot as non-specific targets. Therefore, we proposed a de novo mode of FrameBot for FS correction, which works on a similar basis as common chimera identifying platforms and is not dependent on reference sequences. By nature of FrameBot de novo design, it is crucial to provide it with data as error free as possible. We tested the ability of several publicly available correction tools to decrease the number of errors in the data sets. The combination of Maximum Expected Error (MEE filtering and single linkage pre-clustering (SLP proved the most efficient read procession. Applying FrameBot de novo on the processed data enabled analysis of BphA sequences with minimal losses of potentially functional sequences not homologous to those previously known. This experiment also demonstrated the extensive diversity of dioxygenases in soil. A script which performs FrameBot de novo is presented in the supplementary material to the study and the tool was implemented into FunGene Pipeline available at http://fungene.cme.msu.edu/FunGenePipeline/ and https://github.com/rdpstaff/Framebot.

  16. Large Frame-Transfer Detectors for the MAIA Imager

    CERN Document Server

    Padilla, Jesús Pérez; Raskin, Gert; Prins, Saskia; Merges, Florian; Pessemier, Wim; Bloemen, Steven

    2013-01-01

    MAIA, the Mercator Advanced Imager for Asteroseismology, is a new fast-cadence 3-channel photometric instrument. It is installed on the 1.2-m Mercator telescope at the Roque de Los Muchachos Observatory in La Palma, Spain. MAIA comprises 3 cameras that simultaneously observe the same 9.4 x 14.1 arcmin field in 3 different colour bands (u, g and r). Each camera is based on a very large frame-transfer detector (CCD42-C0) of 2kx6k pixels, specially designed for rapid time-series photometry. These CCDs were originally developed by e2v for ESA's cancelled Eddington space mission and are now on permanent loan to the Institute of Astronomy of the KU Leuven, Belgium. The acquisition system of MAIA uses a single ARC GEN-III controller, custom programmed to allow differing exposure times for each of the three CCDs. Predefined sequences synchronize each read-out with the shortest integration time. Detectors that are not read-out at the end of an exposure continue integrating and can be read-out in one of the subsequent ...

  17. On the Accuracy of Iranian EFL Students' Reading Self-assessment and their Level of Reading Proficiency

    OpenAIRE

    Moein Shokr

    2015-01-01

    Reviewing the literature on self-assessment as an alternative method of assessment we find advocates claiming for the accuracy of the students’ self-assessments in general with little focus on their level of proficiency. With an eye on the students’ level of reading proficiency, the present study aimed at investigating the relationship between students’ reading self-assessment (as a formative and alternative method of assessment) on the one hand, and teacher assessment (as a formative type of...

  18. Alternative RNA Structure-Coupled Gene Regulations in Tumorigenesis

    Directory of Open Access Journals (Sweden)

    Feng-Chi Chen

    2014-12-01

    Full Text Available Alternative RNA structures (ARSs, or alternative transcript isoforms, are critical for regulating cellular phenotypes in humans. In addition to generating functionally diverse protein isoforms from a single gene, ARS can alter the sequence contents of 5'/3' untranslated regions (UTRs and intronic regions, thus also affecting the regulatory effects of these regions. ARS may introduce premature stop codon(s into a transcript, and render the transcript susceptible to nonsense-mediated decay, which in turn can influence the overall gene expression level. Meanwhile, ARS can regulate the presence/absence of upstream open reading frames and microRNA targeting sites in 5'UTRs and 3'UTRs, respectively, thus affecting translational efficiencies and protein expression levels. Furthermore, since ARS may alter exon-intron structures, it can influence the biogenesis of intronic microRNAs and indirectly affect the expression of the target genes of these microRNAs. The connections between ARS and multiple regulatory mechanisms underline the importance of ARS in determining cell fate. Accumulating evidence indicates that ARS-coupled regulations play important roles in tumorigenesis. Here I will review our current knowledge in this field, and discuss potential future directions.

  19. Relaxing Tight Frame Condition in Parallel Proximal Methods for Signal Restoration

    CERN Document Server

    Pustelnik, Nelly; Chaux, Caroline

    2011-01-01

    A fruitful approach for solving signal deconvolution problems consists of resorting to a frame-based convex variational formulation. In this context, parallel proximal algorithms and related alternating direction methods of multipliers have become popular optimization techniques to approximate iteratively the desired solution. Until now, in most of these methods, either Lipschitz differentiability properties or tight frame representations were assumed. In this paper, it is shown that it is possible to relax these assumptions by considering a class of non necessarily tight frame representations, thus offering the possibility of addressing a broader class of signal restoration problems. In particular, it is possible to use non necessarily maximally decimated filter banks with perfect reconstruction, which are common tools in digital signal processing. The proposed approach allows us to solve both frame analysis and frame synthesis problems for various noise distributions. In our simulations, it is applied to th...

  20. READING ALOUD STRATEGIES IN READING ENGLISH TEXTS

    OpenAIRE

    Iyen Nurlaelawati; Shofa Dzulqodah

    2014-01-01

    Abstract: Reading aloud by a young language learner shows unique patterns as the evidence of his/her language data processing. This study, thus, explored the strategies applied by an Indonesian young language learner to read English written texts aloud to identify errors that actually bring certain benefits in her language learning process such as making intelligent guesses when she encountered unfamiliar words. It adopted qualitative case study design involving a seven-year old girl as the s...

  1. Interaction and framing in the performance “Insideout” by Sasha Waltz

    DEFF Research Database (Denmark)

    Thygesen, Mads

    2010-01-01

    This paper examines how Waltz’ performance "Insideout" (2003) on the one hand iterates some relatively stable contextual frames of “sameness” (its mise en scène), but on the other, more radically plays with different and alternating discursive framings or contexts, e.g. “this is theatre” or “this....... This is only partly the case in Waltz’s production, as we shall see in my analysis....

  2. The Impact of Green Framing on Consumers’ Valuations of Energy-Saving Measures

    OpenAIRE

    Harmsen - van Hout, Marjolein; Ghosh, Gaurav; Madlener, Reinhard

    2013-01-01

    It is a matter of interest to policy-makers and marketers alike to quantify the impact of environmentally friendly messaging upon consumer decision-making behavior. We quantify the value of such messaging or 'green framing' through a choice experiment where subjects choose technical, home-related energy saving measures (ESMs) after valuing the attributes of a set of alternatives. Green framing is tested through a treatment where the one half of the subject pool is exposed to a scenario descri...

  3. Evaluation of Reading Support Tools by Reading Comprehension Tests and Reading Speed Tests

    OpenAIRE

    小谷, 克則; 吉見, 毅彦; 九津見, 毅; 佐田, いち子; 井佐原, 均

    2005-01-01

    This paper introduces our reading process monitoring systems and also presents the experimental results that show the adequacy of our reading data. Our system divides a text into reading areas and records reading time for each area. We conducted two experiments using this tool to verify the adequacy of our reading process data. In the first experiment, we examined whether the reading process can distinguish easy text reading and difficult text reading, and confirmed the adequacy of our readin...

  4. Framing Biotechnology in Iranian TV Series

    Directory of Open Access Journals (Sweden)

    H. Khaniki

    2014-07-01

    Full Text Available Media as public opinion formers have crucial role in supporting the growth and development of science and technology . They form media frames in various fields of science and Technology. This paper seeks to identify frames which Iranian television series depict biotechnology. The Biotechnology frames Identified through qualitative framing analysis. To achieve this goal, all TV series of Five main national channels for a five-year period (2008-2013 were considered and two TV series – “Balhayeh Khis, 1392 (Wet Wings,2013 and ” Factoreh hasht, 1387” (Factor 8, 2008 were selected. The central theme in these series was Biotechnology. To identify frames a combination of content analysis and semiotics were used. Findings show that six main frame were used for representation of Biotechnology in Iranian TV series which includes: conspiracy, ideology - spiritual values, scientific progress, ethics, economy and independence.

  5. Exploring the Relationship between Adolescent's Reading Skills, Reading Motivation and Reading Habits

    Science.gov (United States)

    McGeown, Sarah P.; Duncan, Lynne G.; Griffiths, Yvonne M.; Stothard, Sue E.

    2015-01-01

    The present study examines the extent to which adolescents' reading affect (reading motivation) and behaviour (reading habits) predict different components of reading (word reading, comprehension, summarisation and text reading speed) and also adds to the limited research examining group differences (gender, age, ability) in adolescents' reading…

  6. Alternative Treatments

    Science.gov (United States)

    ... Find your chapter: search by state Home > Alzheimer's Disease > Treatments > Alternative Treatments Overview What Is Dementia? What Is Alzheimer's? Younger/Early Onset Facts and Figures Know the 10 Signs Stages Inside the Brain: ...

  7. Frame and Metaphor in Political Games

    OpenAIRE

    Bogost, Ian

    2005-01-01

    This paper offers an approach to analyzing political rhetoric in videogames, and on designing videogames intended to carry ideological bias, based cognitive linguist George Lakoff’s notion of metaphor and frame in political discourse. I argue for two important ways games function in relation to ideological frames, reinforcement and exposition, through examples of art games, political games, and commercial games. Finally, I argue that an explicit design of ideological frames in games is crucia...

  8. Perturbations of Weyl-Heisenberg frames

    OpenAIRE

    Casazza, Peter G.; Christensen, Ole; Lammers, Mark C.

    2000-01-01

    We develop a usable perturbation theory for Weyl-Heisenberg frames. In particular, we prove that if $(E_{mb}T_{na}g)_{m,n\\inmathbb Z}$ is a WH-frame and $h$ is a function which is close to $g$ in the Wiener Amalgam space norm, then $(E_{mb}T_{na}h)_{m,n\\in \\mathbb Z}$ is also a WH-frame.

  9. Journalism Curiosity and Story Telling Frame

    DEFF Research Database (Denmark)

    Grunwald, Ebbe; Rupar, Verica

    2009-01-01

    epistemological and organisational dimension of frames relates to the process of  meaning-making. We suggest refining the concept of frame in journalism studies by making a distinction between a frame (an epistemological category) and an angle (a textual organisation category). Our investigation shows that this...... distinction better serves the analysis and understanding of the mechanisms behind journalism in comparative contexts. Udgivelsesdato: December...

  10. Complex Hadamard matrices and Equiangular Tight Frames

    CERN Document Server

    Szöll\\Hosi, Ferenc

    2011-01-01

    In this paper we give a new construction of parametric families of complex Hadamard matrices of square orders, and connect them to equiangular tight frames. The results presented here generalize some of the recent ideas of Bodmann et al. and extend the list of known equiangular tight frames. In particular, a (36,21) frame coming from a nontrivial cube root signature matrix is obtained for the first time.

  11. Comparative Analysis of Frames with Varying Inertia

    OpenAIRE

    Prerana Nampalli; Prakarsh Sangave

    2015-01-01

    This paper presents an elastic seismic response of reinforced concrete frames with 3 variations of heights, i.e. (G+2), (G+4), (G+6) storey models are compared for bare frame and frame with brick infill structures which have been analyzed for gravity as well as seismic forces and their response is studied as the geometric parameters varying from view point of predicting behavior of similar structures subjected to similar loads or load combinations. In this study, two different cas...

  12. Backreaction: Gauge and Frame Dependences

    CERN Document Server

    Brown, Iain A; Coley, Alan

    2012-01-01

    The cosmological backreaction from perturbations is clearly gauge-dependent, and obviously depends on the choice of averaged Hubble rate. We consider two common choices of Hubble rate and advocate the use of comoving volume-preserving gauges. We highlight two examples valid to an appropriate order in perturbation theory, uniform curvature gauge, which is as close to volume-preserving as possible, and a spatially-traceless uniform cold dark matter gauge which preserves the volume to linear order. We demonstrate the strong gauge- and frame-dependences in averaging. In traceless uniform CDM gauge the backreaction exhibits a strong ultra-violet divergence and can be tuned to an arbitrary magnitude with an appropriate choice of smoothing scale. In uniform curvature gauge we find that for a choice of Hubble rate locked to the spatial surface the backreaction vanishes identically, while for a Hubble rate defined from a fluid's expansion scalar the effective energy density at the current epoch in an Einstein-de Sitte...

  13. Rotating frames in special relativity

    International Nuclear Information System (INIS)

    The transformation theory for rotating frames presented in a previous paper is generalized by replacing the usual condition r = R for ωR < c (invariance of radius) by r = Rg(βsub(R)) so that r is now defined for all values of R, 0 <= R <= infinity. This generalization does not affect the kinematic transformation bracetheta, T → bracethetasup(r), bracesup(r) and the result group structure required by the theoretical constraints previously established, provided the old parameter 'r' (=R) is now identified throughout with either r or R; for physical reasons it must be identified with R. The function g, which cannot be fixed by theoretical constraints, determines the degree of geometrical anisotropy in the rotating plane z = const. More specifically, since g enters the expression for the ratio C/D (circumference/diameter) its choice corresponds to the choice of a congruence definition for lengths in radial and tangential directions. While on this (purely geometrical) level g remains undetermined, it can be uniquely determined experimentally on the kinematic level, e.g. by observing in Σsup(ω) the motion of a free particle. Thus the supremacy of kinematics over geometry is explicated by a further instance. At the same time, special relativity theory (SRT) is shown to belong to the class of theories with theoretically unsolvable problems. (author)

  14. Auto-tuning unit norm frames

    CERN Document Server

    Casazza, Peter G; Mixon, Dustin G

    2010-01-01

    Finite unit norm tight frames provide Parseval-like decompositions of vectors in terms of redundant components of equal weight. They are known to be exceptionally robust against additive noise and erasures, and as such, have great potential as encoding schemes. Unfortunately, up to this point, these frames have proven notoriously difficult to construct. Indeed, though the set of all unit norm tight frames, modulo rotations, is known to contain manifolds of nontrivial dimension, we have but a small finite number of known constructions of such frames. In this paper, we present a new iterative algorithm---gradient descent of the frame potential---for increasing the degree of tightness of any finite unit norm frame. The algorithm itself is trivial to implement, and it preserves certain group structures present in the initial frame. In the special case where the number of frame elements is relatively prime to the dimension of the underlying space, we show that this algorithm converges to a unit norm tight frame at...

  15. Pyramidal space frame and associated methods

    Science.gov (United States)

    Clark, Ryan Michael; White, David; Farr, Jr, Adrian Lawrence

    2016-07-19

    A space frame having a high torsional strength comprising a first square bipyramid and two planar structures extending outward from an apex of the first square bipyramid to form a "V" shape is disclosed. Some embodiments comprise a plurality of edge-sharing square bipyramids configured linearly, where the two planar structures contact apexes of all the square bipyramids. A plurality of bridging struts, apex struts, corner struts and optional internal bracing struts increase the strength and rigidity of the space frame. In an embodiment, the space frame supports a solar reflector, such as a parabolic solar reflector. Methods of fabricating and using the space frames are also disclosed.

  16. Quantum Mechanics in a Rotating Frame

    OpenAIRE

    Anandan, Jeeva; Suzuki, Jun

    2003-01-01

    The rotating frame is considered in quantum mechanics on the basis of the position dependent boost relating this frame to the non rotating inertial frame. We derive the Sagnac phase shift and the spin coupling with the rotation in the non relativistic limit by a simple treatment. By taking the low energy limit of the Dirac equation with a spin connection, we obtain the Hamiltonian for the rotating frame, which gives rise to all the phase shifts discussed before. Furthermore, we obtain a new p...

  17. Pilotless Frame Synchronization Using LDPC Code Constraints

    Science.gov (United States)

    Jones, Christopher; Vissasenor, John

    2009-01-01

    A method of pilotless frame synchronization has been devised for low- density parity-check (LDPC) codes. In pilotless frame synchronization , there are no pilot symbols; instead, the offset is estimated by ex ploiting selected aspects of the structure of the code. The advantag e of pilotless frame synchronization is that the bandwidth of the sig nal is reduced by an amount associated with elimination of the pilot symbols. The disadvantage is an increase in the amount of receiver data processing needed for frame synchronization.

  18. Composite curved frames for helicopter fuselage structure

    Science.gov (United States)

    Rich, M. J.; Lowry, D. W.

    1984-01-01

    This paper presents the results of analysis and testing of composite curved frames. A major frame was selected from the UH-60 Black Hawk helicopter and designed as a composite structure. The curved beam effects were expected to increase flange axial stresses and induce transverse bending. A NASTRAN finite element analysis was conducted and the results were used in the design of composite curved frame specimens. Three specimens were fabricated and five static tests were conducted. The NASTRAN analysis and test results are compared for axial, transverse, and Web strains. Results show the curved beam effects are closely predicted by a NASTRAN analysis and the effects increase with loading on the composite frames.

  19. Attention Therapy Improves Reading Comprehension in Adjudicated Teens in a Residential Facility

    Science.gov (United States)

    Shelley-Tremblay, John; Langhinrichsen-Rohling, Jennifer; Eyer, Joshua

    2012-01-01

    This study quantified the influence of visual Attention Therapy (AT) on reading skills and Coherent Motion Threshold (CMT) in adjudicated teens with moderate reading disabilities (RD) residing in a residential alternative sentencing program. Forty-two students with below-average reading scores were identified using standardized reading…

  20. Effects of Noninformational Color on the Reading Test Performance of Students with and without Attentional Deficits.

    Science.gov (United States)

    Zentall, Sydney S.; Grskovic, Janice A.; Javorsky, James; Hall, Arlene M.

    2000-01-01

    A study involving 25 students (grades 3-5) with and without attentional deficits assessed generality to a standardized reading test when noninformational color was added to one of two alternate forms. Students with attentional deficits read as accurately as their classmates with color added and read worse in the black-white condition. (Contains…

  1. Improve your reading

    CERN Document Server

    Fry, Ron

    2012-01-01

    Help your students discover the practical solution to their reading frustrations, with Improve Your Reading. Written by bestselling author and education advocate Ron Fry, this book avoids gimmicks and tricks in favor of proven strategies that will help your students better retain and comprehend what they've read in any textbook, in any course, at any academic level. Endlessly adaptable to each student's individual learning needs, the text focuses on fundamental skills students can carry beyond the classroom.

  2. Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani.

    Science.gov (United States)

    McNeil, Bonnie A; Simon, Dawn M; Zimmerly, Steven

    2014-02-01

    Group II introns are ribozymes and retroelements found in bacteria, and are thought to have been the ancestors of nuclear pre-mRNA introns. Whereas nuclear introns undergo prolific alternative splicing in some species, group II introns are not known to carry out equivalent reactions. Here we report a group II intron in the human pathogen Clostridium tetani, which undergoes four alternative splicing reactions in vivo. Together with unspliced transcript, five mRNAs are produced, each encoding a distinct surface layer protein isoform. Correct fusion of exon reading frames requires a shifted 5' splice site located 8 nt upstream of the canonical boundary motif. The shifted junction is accomplished by an altered IBS1-EBS1 pairing between the intron and 5' exon. Growth of C. tetani under a variety of conditions did not result in large changes in alternative splicing levels, raising the possibility that alternative splicing is constitutive. This work demonstrates a novel type of gene organization and regulation in bacteria, and provides an additional parallel between group II and nuclear pre-mRNA introns. PMID:24214997

  3. On Reading Test

    Institute of Scientific and Technical Information of China (English)

    孙健

    2005-01-01

    There has been a long discussion over the construct validity of reading tests. In china's reading tests, multiple choice is the main test method in view of the4 long controversy over the validity of multiple choice, construct validation is called for to empirically test the hypothesized relationships between test scores and abilities. The national CET committee conducted a comprehensive validation study. As part of the project, the specialists studied the reading comprehension test's validity by qualitative means, namely "introspective verbal reports". The analysis revealed that an overwhelming majority of the questions items were handled through "expected reading operations".

  4. The Reading Brain

    OpenAIRE

    Kassuba, Tanja; Kastner, Sabine

    2015-01-01

    Do you enjoy reading books? Reading is one of the unique activities that only humans do, and we have not been doing it for that long! Humans have talked to each other using a language system with grammatical rules for at least 100,000 years, but we have been reading and writing only for a few thousand years! What happens in our brain when we read? Our brain has developed a region that is specialized in knowing what written words look like. It closely works together with other parts of the bra...

  5. Alternative splicing of exon 17 and a missense mutation in exon 20 of the insulin receptor gene in two brothers with a novel syndrome of insulin resistance (congenital fiber-type disproportion myopathy)

    DEFF Research Database (Denmark)

    Vorwerk, P; Christoffersen, C T; Müller, J;

    1999-01-01

    compound heterozygotes for mutations in the IR gene. The maternal allele was alternatively spliced in exon 17 due to a point mutation in the -1 donor splice site of the exon. The abnormal skipping of exon 17 shifts the amino acid reading frame and leads to a truncated IR, missing the entire tyrosine kinase...... domain. In the correct spliced variant, the point mutation is silent and results in a normally translated IR. The paternal allele carries a missense mutation in the tyrosine kinase domain. All three cDNA variants were present in the lymphocytes of the patients. Purified IR from 293 cells overexpressing...

  6. The Conversational Frame in Public Address.

    Science.gov (United States)

    Branham, Robert James; Pearce, W. Barnett

    1996-01-01

    Explores the diverse forms and motives of the conversational frame in public address. Argues that, by framing their remarks and transactions with their listeners as conversational, orators may attempt to reconstruct or seem to reconstruct speaker-audience relationships and to position themselves and their audiences within networks of reciprocal…

  7. Sustainable food and agriculture: stakeholder's frames

    NARCIS (Netherlands)

    B. van Gorp; M.J. van der Goot

    2012-01-01

    Despite its importance, the notion of sustainability is open for discursive struggle. This article's primary objective is to acquire insight into the manner in which the principal stakeholders strategically use frames in their public communication about sustainable food and agriculture. A framing an

  8. Teaching the Dynamics of Framing Competitions

    Science.gov (United States)

    Rinke, Eike Mark

    2012-01-01

    Framing theory is one of the most thriving and complex fields of communication theory, and as such it has grown to be an integral part of many political communication, public opinion, and communication theory courses. Part of the complexity stems from scholars' efforts to develop accounts of framing processes that are closer to the "real world" of…

  9. Dynamics of the Frame in Visual Composition.

    Science.gov (United States)

    Herbener, Gerald F.; And Others

    1979-01-01

    Forty-four college students rated six framed, black-and-white single object pictures to determine if the framing of an object or the field surrounding it gives it more meaning. Based on factor analysis of the results, recommendations are made for future research. (JEG)

  10. Characterization of Oblique Dual Frame Pairs

    DEFF Research Database (Denmark)

    Christensen, Ole; Eldar, Yonina

    characterization which in some cases can be computationally more efficient. We first treat the case of a general frame on an arbitrary Hilbert space, and then specialize the results to shift-invariant frames with multiple generators. In particular, we present explicit versions of our general conditions for the...

  11. Gestural Manifestation of Knowledge in Conceptual Frames

    Science.gov (United States)

    Chui, Kawai

    2012-01-01

    Frames are cognitive structures of knowledge grounded in people's social interaction in recurrent sociocultural activities or individual incidences. Discussion concerning the knowledge in frames largely focuses on its linguistic manifestation with regard to roles and role relations in scenes. Little attention has been paid to the scriptal…

  12. An introduction to frames and Riesz bases

    CERN Document Server

    Christensen, Ole

    2016-01-01

    This revised and expanded monograph presents the general theory for frames and Riesz bases in Hilbert spaces as well as its concrete realizations within Gabor analysis, wavelet analysis, and generalized shift-invariant systems.  Compared with the first edition, more emphasis is put on explicit constructions with attractive properties.  Based on the exiting development of frame theory over the last decade, this second edition now includes new sections on the rapidly growing fields of LCA groups, generalized shift-invariant systems, duality theory for as well Gabor frames as wavelet frames, and open problems in the field.   Key features include: *Elementary introduction to frame theory in finite-dimensional spaces * Basic results presented in an accessible way for both pure and applied mathematicians * Extensive exercises make the work suitable as a textbook for use in graduate courses * Full proofs includ ed in introductory chapters; only basic knowledge of functional analysis required * Explicit constructi...

  13. Cosmic alternatives?

    Science.gov (United States)

    Gregory, Ruth

    2009-04-01

    "Cosmologists are often in error but never in doubt." This pithy characterization by the Soviet physicist Lev Landau sums up the raison d'être of Facts and Speculations in Cosmology. Authors Jayant Narlikar and Geoffrey Burbidge are proponents of a "steady state" theory of cosmology, and they argue that the cosmological community has become fixated on a "Big Bang" dogma, suppressing alternative viewpoints. This book very much does what it says on the tin: it sets out what is known in cosmology, and puts forward the authors' point of view on an alternative to the Big Bang.

  14. Adjudicating Frame Shifts and Frame Disputes in the New Millennial University: The Role of the Dean

    Science.gov (United States)

    Lessor, Roberta G.

    2008-01-01

    This paper argues that sociological theory provides a sound basis for analyzing the social organization and reorganization of the college or university and for guiding the activities of the college dean. Frame analysis theory, developed in the study of social movements, and the attendant concepts of frame shifts and frame disputes, are drawn on to…

  15. A School Policy for Reading.

    Science.gov (United States)

    Medwell, Jane

    1992-01-01

    Offers a school policy for reading, arrived at as a product of a whole school inservice program. Discusses word recognition, sound-symbol correspondence, prediction, classroom environment, reading with an adult, parents and reading, assessment of reading, and reading material selection. (RS)

  16. Normal and Compound Poisson Approximations for Pattern Occurrences in NGS Reads

    OpenAIRE

    Zhai, Zhiyuan; Reinert, Gesine; Song, Kai; Waterman, Michael S.; Luan, Yihui; Sun, Fengzhu

    2012-01-01

    Next generation sequencing (NGS) technologies are now widely used in many biological studies. In NGS, sequence reads are randomly sampled from the genome sequence of interest. Most computational approaches for NGS data first map the reads to the genome and then analyze the data based on the mapped reads. Since many organisms have unknown genome sequences and many reads cannot be uniquely mapped to the genomes even if the genome sequences are known, alternative analytical methods are needed fo...

  17. TruSPAdes: barcode assembly of TruSeq synthetic long reads.

    Science.gov (United States)

    Bankevich, Anton; Pevzner, Pavel A

    2016-03-01

    The recently introduced TruSeq synthetic long read (TSLR) technology generates long and accurate virtual reads from an assembly of barcoded pools of short reads. The TSLR method provides an attractive alternative to existing sequencing platforms that generate long but inaccurate reads. We describe the truSPAdes algorithm (http://bioinf.spbau.ru/spades) for TSLR assembly and show that it results in a dramatic improvement in the quality of metagenomics assemblies. PMID:26828418

  18. HMM-FRAME: accurate protein domain classification for metagenomic sequences containing frameshift errors

    Directory of Open Access Journals (Sweden)

    Sun Yanni

    2011-05-01

    Full Text Available Abstract Background Protein domain classification is an important step in metagenomic annotation. The state-of-the-art method for protein domain classification is profile HMM-based alignment. However, the relatively high rates of insertions and deletions in homopolymer regions of pyrosequencing reads create frameshifts, causing conventional profile HMM alignment tools to generate alignments with marginal scores. This makes error-containing gene fragments unclassifiable with conventional tools. Thus, there is a need for an accurate domain classification tool that can detect and correct sequencing errors. Results We introduce HMM-FRAME, a protein domain classification tool based on an augmented Viterbi algorithm that can incorporate error models from different sequencing platforms. HMM-FRAME corrects sequencing errors and classifies putative gene fragments into domain families. It achieved high error detection sensitivity and specificity in a data set with annotated errors. We applied HMM-FRAME in Targeted Metagenomics and a published metagenomic data set. The results showed that our tool can correct frameshifts in error-containing sequences, generate much longer alignments with significantly smaller E-values, and classify more sequences into their native families. Conclusions HMM-FRAME provides a complementary protein domain classification tool to conventional profile HMM-based methods for data sets containing frameshifts. Its current implementation is best used for small-scale metagenomic data sets. The source code of HMM-FRAME can be downloaded at http://www.cse.msu.edu/~zhangy72/hmmframe/ and at https://sourceforge.net/projects/hmm-frame/.

  19. From home safety to international terrorism: how Italian talk shows framed migration after the Paris attacks

    OpenAIRE

    Giovanni Brancato; Alessandro Ricci; Melissa Stolfi

    2016-01-01

    This article investigates the migrants' representation in the Italian talk shows and how new reading frames are developed after the Paris terrorist attacks in November 2015. The theoretical framework is composed by studies on migrants' representation in media. After recognition of all the primetime Italian talk shows that have been broadcasted in the period between 14 November 2015 and 14 January 2016, we have chosen three: Ballarò, Quinta Colonna e Piazza Pulita. The selected corpus has been...

  20. Reading Authentic Texts

    DEFF Research Database (Denmark)

    Balling, Laura Winther

    2013-01-01

    Most research on cognates has focused on words presented in isolation that are easily defined as cognate between L1 and L2. In contrast, this study investigates what counts as cognate in authentic texts and how such cognates are read. Participants with L1 Danish read news articles in their highly...

  1. Reading and Perestroika.

    Science.gov (United States)

    Plotnikov, Sergei N.

    1992-01-01

    Presents a short historical and sociological analysis of reading in the Soviet Union from the beginning of the twentieth century to perestroika. Discusses some sociocultural problems associated with reading, including the prevailing social, economic and political crises in all spheres of life, particularly the cultural. (RS)

  2. EXPERIMENTS IN KINDERGARTEN READING.

    Science.gov (United States)

    BAKER, EVA L.; AND OTHERS

    FOUR PAPERS GIVEN AT THE READING SYMPOSIUM OF THE 1968 AMERICAN EDUCATIONAL RESEARCH ASSOCIATION PRESENT DISCUSSIONS OF THE SOUTHWEST REGIONAL LABORATORY (SWRL) READING PROGRAM. THE FIRST PAPER DESCRIBES OBJECTIVES, CLASSROOM MATERIALS, AND PROCEDURES. OBJECTIVES FOR SIGHT-RECOGNITION, WORD-ATTACK SKILLS, AND COMPREHENSION SKILLS ARE EXPRESSED IN…

  3. Using Collaborative Strategic Reading.

    Science.gov (United States)

    Klingner, Janette K.; Vaughn, Sharon

    1998-01-01

    Describes collaborative strategic reading (CSR), a technique for teaching students, such as those with learning disabilities, reading comprehension and vocabulary skills in a cooperative setting. Covers teaching the four strategies of CSR (preview, click and clunk, get the gist, and wrap up), as well as teaching students cooperative learning group…

  4. Read before you cite!

    OpenAIRE

    Simkin, M. V.; Roychowdhury, V. P.

    2002-01-01

    We report a method of estimating what percentage of people who cited a paper had actually read it. The method is based on a stochastic modeling of the citation process that explains empirical studies of misprint distributions in citations (which we show follows a Zipf law). Our estimate is only about 20% of citers read the original.

  5. Superheroes and Summer Reading.

    Science.gov (United States)

    Meyer, Ron; Buckner, Joyce

    1980-01-01

    To combat summer learning loss among remedial readers, teachers and consultants in the Omaha, Nebraska, Title I program designed a series of comic-book reading units and mailed them to students' homes. Parents were pleased with the project and it appeared that less reading skill had been lost by September. (SJL)

  6. Maryland Reading First.

    Science.gov (United States)

    Maryland State Dept. of Education, Baltimore.

    In years past, Maryland has provided little guidance toward adoption and implementation of Scientifically Based Reading Research (SBRR) programs. Only recently, with the implementation of the Comprehensive School Reform Demonstration program and the Reading Excellence Act (REA) program, have schools or local education agencies been required to…

  7. Reading Where It Counts

    Science.gov (United States)

    Miller, Harry

    2014-01-01

    In this article, teachers are reminded that their content subject areas require acquainting children with special words or symbols related to that subject area (e.g. mathematics or social studies). Because children can read well does not mean they would be understanding of any special reading skill required in a content subject area; that the…

  8. Measuring Reading Performance Informally.

    Science.gov (United States)

    Powell, William R.

    To improve the accuracy of the informal reading inventory (IRI), a differential set of criteria is necessary for both word recognition and comprehension scores for different levels and reading conditions. In initial evaluation, word recognition scores should reflect only errors of insertions, omissions, mispronunciations, substitiutions, unkown…

  9. Framing the Problem of Radioactive Waste: Public and Institutional Perspectives

    Energy Technology Data Exchange (ETDEWEB)

    Hunt, Jane [Lancaster Univ. (United Kingdom). Centre for the Study of Environmental Change

    2001-07-01

    frame the issue of radioactive waste in the current context of UK society. The author presents the method as a useful, practical and cost effective way of addressing questions of framing, and goes on to argue that not only is this essential as a first stage of developing policies, but that framing needs to be re-assessed over time to enable changes to be mapped and incorporated into policy. The results of the study show clearly the use the general public make of their particular cultural resources in arguing towards a position on what the issues are and how they should be managed, including multiple references to other issues which are seen as similar or having similar components. The study also showed a considerable degree of congruence between different groups, providing a valuable starting point for the re-evaluation of UK radioactive waste management policy. The findings are related to other contemporary studies in the UK, which generally support the overall findings. A problem is identified, however, in that responsible institutions, with their existing practices of understanding the nature of the problem, can find it difficult to integrate the both the concept of framing and the alternatives understandings of the problem which are articulated through ration.

  10. Framing the Problem of Radioactive Waste: Public and Institutional Perspectives

    International Nuclear Information System (INIS)

    radioactive waste in the current context of UK society. The author presents the method as a useful, practical and cost effective way of addressing questions of framing, and goes on to argue that not only is this essential as a first stage of developing policies, but that framing needs to be re-assessed over time to enable changes to be mapped and incorporated into policy. The results of the study show clearly the use the general public make of their particular cultural resources in arguing towards a position on what the issues are and how they should be managed, including multiple references to other issues which are seen as similar or having similar components. The study also showed a considerable degree of congruence between different groups, providing a valuable starting point for the re-evaluation of UK radioactive waste management policy. The findings are related to other contemporary studies in the UK, which generally support the overall findings. A problem is identified, however, in that responsible institutions, with their existing practices of understanding the nature of the problem, can find it difficult to integrate the both the concept of framing and the alternatives understandings of the problem which are articulated through ration

  11. Growing Alternatives

    DEFF Research Database (Denmark)

    Bagger-Petersen, Mai Corlin

    2014-01-01

    From 2014, Anhui Province will pilot a reform of the residential land market in China, thus integrating rural Anhui in the national housing market. In contrast, artist and activist Ou Ning has proposed the Bishan time money currency, intending to establish an alternative economic circuit in Bishan...

  12. Framework for risk analysis in Multimedia Environmental Systems (FRAMES)

    Energy Technology Data Exchange (ETDEWEB)

    Whelan, G.; Buck, J.W.; Castleton, K.J.; Hoopes, B.L.; Pelton, M.A.; McDonald, J.P.; Gelston, G.M.; Taira, R.Y. [Pacific Northwest National Lab., Richland, WA (United States)

    1998-05-01

    The objectives of this workshop are to (1) provide the NRC staff and the public with an overview of currently available Federally-Sponsored dose models appropriate for decommissioning assessments and (2) discuss NRC staff-developed questions related to model selection criteria with the final rule on ``Radiological Criteria for License Termination`` (62 FR 39058). For over 40 years, medium specific models have been and will continue to be developed in an effort to understand and predict environmental phenomena, including fluid-flow patterns, contaminant migration and fate, human or wildlife exposures, impacts from specific toxicants to specific species and their organs, cost-benefit analyses, impacts from remediation alternatives, etc. For nearly 40 years, medium-specific models have been combined for either sequential or concurrent assessments. The evolution of multiple-media assessment tools has followed a logic progression. To allow a suite of users the flexibility and versatility to construct, combine, and couple attributes that meet their specific needs without unnecessarily burdening the user with extraneous capabilities, the development of a computer-based methodology to implement a Risk Analysis in Multimedia Environmental Systems (FRAMES) was begun in 1994. FRAMES represents a platform which links elements together and yet does not represent the models that are linked to or within it; therefore, changes to elements that are linked to or within FRAMES do not change the framework.

  13. Technological Difficulties: A Theoretical Frame for Understanding the Non-Relativistic Permanence of Traditional Print Literacy in Elementary Education

    Science.gov (United States)

    Hassett, Dawnene D.

    2006-01-01

    Currently, definitions of "science", "reading", and "literacy" in the US lend a seemingly nonrelativistic permanence to these terms, and render them resistant to critique. This paper offers a theoretical frame for critiquing this permanence, analysing why early-literacy instruction is tightly tied to traditional forms of print literacy, focusing…

  14. Framing the Genetics Curriculum for Social Justice: An Experimental Exploration of How the Biology Curriculum Influences Beliefs about Racial Difference

    Science.gov (United States)

    Donovan, Brian M.

    2016-01-01

    This field experiment manipulated the racial framing of a reading on human genetic disease to explore whether racial terminology in the biology curriculum affects how adolescents explain and respond to the racial achievement gap in American education. Carried out in a public high school in the San Francisco Bay Area, students recruited for the…

  15. Grasp Reading Skills to Improve Reading Ability?

    Institute of Scientific and Technical Information of China (English)

    CaoJingt

    2004-01-01

    Reading is a kind of communication, and for most people in most situations it is more important than speaking. For college students today, it is more important for them to obtain the newest information on their own fields through English,rather than showing their English certificates. However, the most common problem students have nowadays, including

  16. The relation between strategic reading strategies and reading comprehension.

    OpenAIRE

    Schooten, E. van; Bimmel, P.

    2004-01-01

    In the present article, we investigate the degree to which 15-year-old students in regular secondary education in the Netherlands (N = 144) master four strategic reading activities and the relationship of the mastery to their first-language reading comprehension, preference for self-direction and in-depth information processing, and attitude towards reading and strategic reading activities. Students’ mastery of strategic reading activities appears to be strongly related to reading comprehensi...

  17. The Importance of Metacognitive Reading Strategy Awareness in Reading Comprehension

    OpenAIRE

    Mohammad Reza Ahmadi; Hairul Nizam Ismail; Muhammad Kamarul Kabilan Abdullah

    2013-01-01

    Metacognitive reading strategy awareness plays a significant role in reading comprehension and educational process. In spite of its importance, metacognitive strategy has long been the ignored skill in English language teaching, research, learning, and assessment. This lack of good metacognitive reading strategy skill is exacerbated by the central role of reading comprehension in education success. One solution to the problem of poor reading comprehension is the learning of metacognitive read...

  18. High frame rate measurements of semiconductor pixel detector readout IC

    International Nuclear Information System (INIS)

    We report on high count rate and high frame rate measurements of a prototype IC named FPDR90, designed for readouts of hybrid pixel semiconductor detectors used for X-ray imaging applications. The FPDR90 is constructed in 90 nm CMOS technology and has dimensions of 4 mm×4 mm. Its main part is a matrix of 40×32 pixels with 100 μm×100 μm pixel size. The chip works in the single photon counting mode with two discriminators and two 16-bit ripple counters per pixel. The count rate per pixel depends on the effective CSA feedback resistance and can be set up to 6 Mcps. The FPDR90 can operate in the continuous readout mode, with zero dead time. Due to the architecture of digital blocks in pixel, one can select the number of bits read out from each counter from 1 to 16. Because in the FPDR90 prototype only one data output is available, the frame rate is 9 kfps and 72 kfps for 16 bits and 1 bit readout, respectively (with nominal clock frequency of 200 MHz).

  19. A journey through alternative ways of living

    OpenAIRE

    Baroncelli Torretta, Nicholas

    2014-01-01

    This master thesis examines how an evolution towards sustainable ways of urban living can be achieved through small steps. In this way, a literature review on the fields of design, sustainability and alternative ways of living provides a frame for approaching design for sustainability as well as an historical overview on alternative social movements and its motivations. Consequently, a field research proves Helsinki area as a powerful agent on practices informing sustainable futures. This are...

  20. A spatial reference frame model of Beijing based on spatial cognitive experiment

    Science.gov (United States)

    Zhang, Jie; Zhang, Jing; Liu, Yu

    2006-10-01

    Orientation relation in the spatial relation is very important in GIS. People can obtain orientation information by making use of map reading and the cognition of the surrounding environment, and then create the spatial reference frame. City is a kind of special spatial environment, a person with life experiences has some spatial knowledge about the city where he or she lives in. Based on the spatial knowledge of the city environment, people can position, navigate and understand the meaning embodied in the environment correctly. Beijing as a real geographic space, its layout is very special and can form a kind of new spatial reference frame. Based on the characteristics of the layout of Beijing city, this paper will introduce a new spatial reference frame of Beijing and use two psychological experiments to validate its cognitive plausibility.

  1. Role of Reading Engagement in Mediating Effects of Reading Comprehension Instruction on Reading Outcomes

    Science.gov (United States)

    Wigfield, Allan; Guthrie, John T.; Perencevich, Kathleen C.; Taboada, Ana; Klauda, Susan Lutz; McRae, Angela; Barbosa, Pedro

    2008-01-01

    The engagement model of reading development suggests that instruction improves students' reading comprehension to the extent that it increases students' engagement processes in reading. We compared how Concept-Oriented Reading Instruction (CORI) (support for cognitive and motivational processes in reading), strategy instruction (support for…

  2. Positive Operator Valued Measures: A General Setting for Frames

    OpenAIRE

    Moran, Bill; Howard, Stephen; Cochran, Doug

    2011-01-01

    This paper presents an overview of close parallels that exist between the theory of positive operator-valued measures (POVMs) associated with a separable Hilbert space and the theory of frames on that space, including its most important generalizations. The concept of a framed POVM is introduced, and classical frames, fusion frames, generalized frames, and other variants of frames are all shown to to arise as framed POVMs. This observation allows drawing on a rich existing theory of POVMs to ...

  3. Discourse Awareness and Teaching Reading

    Institute of Scientific and Technical Information of China (English)

    FEI Jun

    2014-01-01

    Reading is an interactive process and discourse knowldege facilitates interpretation of the text. This paper discusses the thoery of reading from a discouse apporach, explores the interactive approach of teaching reading and discusses pedagogical impi-cations with contexts concerned.

  4. Simple Design Tool for Development of Well Insulated Window Frames and Optimization of the Frame Geometry

    DEFF Research Database (Denmark)

    Zajas, Jan Jakub; Heiselberg, Per

    2012-01-01

    This paper describes a design tool created with the purpose of designing highly insulated window frames. The design tool is based on a parametric model of the frame geometry, where various parameters describing the frame can be easily changed by the user. Based on this input, geometry of the frame...... is generated by the program and is used by the finite element simulator to calculate the thermal performance of the frame (the U value). After the initial design is evaluated, the user can quickly modify chosen parameters and generate a new design. This process can then be repeated in multiple...... iterations in order to approach an optimal solution. The program was also used to conduct an optimization process of the frame geometry. A large number of various window frame designs were created and evaluated, based on their insulation properties. The paper presents the investigation process and some of...

  5. Quantum Stackelberg Duopoly in a Noninertial Frame

    Institute of Scientific and Technical Information of China (English)

    Salman Khan; M. Khalid Khan

    2011-01-01

    We study the influence of the Unruh effect on quantum Stackelberg duopoly.It is shown that the acceleration of a noninertial frame strongly affects the payoffs of the firms. The validation of the subgame perfect Nash equilibrium is limited to a particular range of acceleration of the noninertial frame.The benefit of the initial state entanglement in the quantum form of the duopoly in the inertial frame is adversely affected by the acceleration.The duopoly can become a follower advantage only in a small region of the acceleration.

  6. Groups, cacti and framed little discs

    CERN Document Server

    Hepworth, Richard

    2010-01-01

    Let G be a topological group. Then the based loopspace of G is an algebra over the cacti operad, while the double loopspace of the classifying space of G is an algebra over the framed little discs operad. This paper shows that these two algebras are equivalent, in the sense that they are weakly equivalent E-algebras, where E is an operad weakly equivalent to both framed little discs and cacti. We recover the equivalence between cacti and framed little discs, and Menichi's isomorphism between the BV-algebras obtained by taking the homology of the loopspace of G and of the double loopspace of BG.

  7. The Art of Re-Framing

    OpenAIRE

    Ana Navarro Wagner

    2015-01-01

    This article exposes a way of re-framing the experiences of four European music therapists with local disadvantaged children in Gulu (North of Uganda, Africa) from a Community Music Therapy point of view. CoMT offers certain qualities that help to rethink conventional bounderies in their contexts and to widen the cultural sensitivity of the music therapists. The power of re-framing according to the context is the power of looking with other frames, the power of allowing other patterns to arise.

  8. The Art of Re-Framing

    Directory of Open Access Journals (Sweden)

    Ana Navarro Wagner

    2015-01-01

    Full Text Available This article exposes a way of re-framing the experiences of four European music therapists with local disadvantaged children in Gulu (North of Uganda, Africa from a Community Music Therapy point of view. CoMT offers certain qualities that help to rethink conventional bounderies in their contexts and to widen the cultural sensitivity of the music therapists. The power of re-framing according to the context is the power of looking with other frames, the power of allowing other patterns to arise.

  9. Framing of regimes and transition strategies

    DEFF Research Database (Denmark)

    Jensen, Jens Stissing

    2012-01-01

    This article suggests that transition strategies are always formulated in the context of specific representations of the regime and the challenges it faces. It is argued that the framing of a regime affects the envisioning of transition strategies. An analysis of the current development agenda for...... the housing construction sector in Denmark reveals the relevance and impacts of different regime framings. It is proposed that the ability to cope with framing issues as situated and political processes is at the core of the governance of transitions....

  10. Frame-Dragging from Charged Rotating Body

    CERN Document Server

    Dubey, Anuj Kumar

    2016-01-01

    In the present paper, we have considered the three parameters: mass, charge and rotation to discuss their combined effect on frame dragging for a charged rotating body. If we consider the ray of light which is emitted radially outward from a rotating body then the frame dragging shows a periodic nature with respect to coordinate $\\phi$ (azimuthal angle). It has been found that the value of frame dragging obtains a maximum at, $ \\phi =\\frac{\\pi}{2}$ and a minimum at $ \\phi =\\frac{3 \\pi}{2}$.

  11. Elastoplastic analysis of knee bracing frame

    Institute of Scientific and Technical Information of China (English)

    HUANG Zhen; LI Qing-song; CHEN Long-zhu

    2005-01-01

    The knee bracing steel frame (KBF) is a new kind of energy dissipating frame, which combines excellent ductility and lateral stiffness. As the structural fuse of the frame, the knee element will yield first during a severe earthquake so that no damage occurs to the major structural members and the rehabilitation is easy and economical. To help fully understand the relations between its seismic performance and the structural parameters, systematic elastoplastic analysis of the KBF structure with finite element method was conducted in this work. Finally, general design recommendations were made according to the results of the analysis.

  12. Alternative Design of Postgraduate English Writing Course

    Institute of Scientific and Technical Information of China (English)

    王芬

    2008-01-01

    This paper presents an alternative design of postgraduate English writing course to meet the requirement of the current postgraduate English teaching reform.The author believes that efficiently-organized writing activities based on practical needs could refresh teaching frame- work and consequently assist postgraduates in English writing.

  13. Metacognition and Reading Comprehension

    Institute of Scientific and Technical Information of China (English)

    Cecilia Chang

    2000-01-01

    @@ Introduction Reading is a mysterious process that has attracted attention from psychologists, reading researchers and educators alike for decades. Currently, reading is viewed as a meaningconstructing process where the reader interacts with the text by simultaneously using information from a variety of sources ranging from one's background knowledge of the content and about the world to the knowledge about the language in which the text is written (Mulling, 1994). Moreover, reading comprehension is achieved only after skillful orchestration of all the resources the reader possess when engaged in the act of reading. Among the various kinds of strategies a reader needs during the reading process are the monitoring strategies. In order to fully utilize the strategies, the reader needs to possess good metacognition. Metacognition refers to one's deliberate conscious control of one's cognition actions (Brown, 1980). Since it is crucial for success that we know what we know and what we don't know and, consequently what to do about what we know and don't know, it is not surprising to find the notion of metacognition being stressed in almost every situation of learning. Given the importance of metacognition in successful learning, the purpose of this paper is to explore the role metacognition plays in reading comprehension, and consequently, identify future research directions.

  14. Metal-framed partitions with reduced thicknesses. Part 1: Narrower studs and cavities

    Science.gov (United States)

    Plumb, G. D.

    The sound insulations were measured, in the Research Department Transmission Suite, of ten different metal-framed partitions. These partitions had narrower cavities and metal studs than those of the conventional thickness metal-framed Camden. Mineral wool had been installed in the cavities of some of the partitions and some partitions had double plasterboard skins rather than plasterboard-fiberboard skins. The sound insulations of these narrow partitions were generally comparable with, or marginally higher than, those of similar partitions, having stud and cavity widths equal to those in the conventional thickness metal-framed Camden. However, the loadbearing capabilities must be investigated before these narrow partitions can be recommended as alternatives to the conventional thickness metal-framed Camden. The use of these narrow partitions should result in average increases, of approximately 8%, in the available floor areas of typical studios.

  15. Alternative 23

    OpenAIRE

    Jackson, Mark

    2014-01-01

    Alternative 23 is a curated exhibition of works by Steve Aylett, David Blandy & Daniel Locke, Let Me Feel Your Finger First, Laura Oldfield Ford, Plastique Fantastique and Henrik Schrat, including the first screening of Let Me Feel Your Finger First’s Postcolonial Capers. In 1985 DC Comics in the US had taken the commercial decision to unify the complex and contradictory character story arcs from its various strips such as Superman, Batman and Green Lantern. The resultant crossover series...

  16. Interruptions disrupt reading comprehension.

    Science.gov (United States)

    Foroughi, Cyrus K; Werner, Nicole E; Barragán, Daniela; Boehm-Davis, Deborah A

    2015-06-01

    Previous research suggests that being interrupted while reading a text does not disrupt the later recognition or recall of information from that text. This research is used as support for Ericsson and Kintsch's (1995) long-term working memory (LT-WM) theory, which posits that disruptions while reading (e.g., interruptions) do not impair subsequent text comprehension. However, to fully comprehend a text, individuals may need to do more than recognize or recall information that has been presented in the text at a later time. Reading comprehension often requires individuals to connect and synthesize information across a text (e.g., successfully identifying complex topics such as themes and tones) and not just make a familiarity-based decision (i.e., recognition). The goal for this study was to determine whether interruptions while reading disrupt reading comprehension when the questions assessing comprehension require participants to connect and synthesize information across the passage. In Experiment 1, interruptions disrupted reading comprehension. In Experiment 2, interruptions disrupted reading comprehension but not recognition of information from the text. In Experiment 3, the addition of a 15-s time-out prior to the interruption successfully removed these negative effects. These data suggest that the time it takes to process the information needed to successfully comprehend text when reading is greater than that required for recognition. Any interference (e.g., an interruption) that occurs during the comprehension process may disrupt reading comprehension. This evidence supports the need for transient activation of information in working memory for successful text comprehension and does not support LT-WM theory. PMID:25867225

  17. Newspaper Reading and English Teaching

    Institute of Scientific and Technical Information of China (English)

    霍丽蓉

    2014-01-01

    Reading is not only the most effective way to get the knowledge of the language but also the only way to improve the language skills of English learners. However, the vast majority of students' reading ability are far apart from the curriculum stan⁃dards in reality. Reading English newspaper helps to mobilize the enthusiasm of students' reading, and improve their reading abili⁃ty. It forms a bridge between learning and real life. It extends the English reading from the teaching materials and guides students to a lot of extracurricular reading, creating a new reading space for students.

  18. The Componential Model of Reading: Predicting First Grade Reading Performance of Culturally Diverse Students From Ecological, Psychological, and Cognitive Factors Assessed at Kindergarten Entry

    OpenAIRE

    Hernandez, Miriam; Folsom, Jessica S.; Otaiba, Stephanie Al; Greulich, Luana; Thomas-Tate, Shurita; Connor, Carol M

    2012-01-01

    This study, framed by the component model of reading (CMR), examined the relative importance of kindergarten-entry predictors of first grade reading performance. Specifically, elements within the ecological domain included dialect, maternal education, amount of preschool, and home literacy; elements within the psychological domain included teacher-reported academic competence, social skills, and behavior; and elements within the cognitive domain included initial vocabulary, phonological, and ...

  19. Lenses on Reading An Introduction to Theories and Models

    CERN Document Server

    Tracey, Diane H

    2012-01-01

    This widely adopted text explores key theories and models that frame reading instruction and research. Readers learn why theory matters in designing and implementing high-quality instruction and research; how to critically evaluate the assumptions and beliefs that guide their own work; and what can be gained by looking at reading through multiple theoretical lenses. For each theoretical model, classroom applications are brought to life with engaging vignettes and teacher reflections. Research applications are discussed and illustrated with descriptions of exemplary studies. New to This Edition

  20. Myths, Misconceptions, and Misunderstandings: A Different Spin on Coriolis--Applying Frame of Reference

    Science.gov (United States)

    DiSpezio, Michael A.

    2011-01-01

    This article addresses misconceptions surrounding the Coriolis force and describes how it should be presented as a function within inertial and noninertial frames of reference. Not only does this demonstrate the nature of science as it strives to best interpret the natural world (and presents alternative explanations), but it offers a rich…

  1. Lifting scheme of symmetric tight wavelets frames

    Institute of Scientific and Technical Information of China (English)

    ZHUANG BoJin; YUAN WeiTao; PENG LiZhong

    2008-01-01

    This paper proposes a method to realize the lifting scheme of tight frame wavelet filters. As for 4-channel tight frame wavelet filter, the tight frame transforms' ma-trix is 2×4, but the lifting scheme transforms' matrix must be 4×4. And in the case of 3-channel tight frame wavelet filter, the transforms' matrix is 2×3, but the lifting scheme transforms' matrix must be 3×3. In order to solve this problem, we intro-duce two concepts: transferred polyphase matrix for 4-channel filters and trans-ferred unitary matrix for 3-channel filters. The transferred polyphase matrix is sym-metric/antisymmetric. Thus, we use this advantage to realize the lifting scheme.

  2. News Media Framing of Negative Campaigning

    DEFF Research Database (Denmark)

    Pedersen, Rasmus Tue

    2014-01-01

    News media coverage of election campaigns is often characterized by use of the strategic game frame and a focus on politicians’ use of negative campaigning. However, the exact relationship between these two characteristics of news coverage is largely unexplored. This article theorizes that consumer...... demand and norms of journalistic independence might induce the news media outlets to cover negative campaigning with a strategic game frame. A comprehensive content analysis based on several newspaper types, several election campaigns, and several different measurements of media framing confirms...... that news coverage of negative campaigning does apply the strategic game frame to a significantly larger degree than articles covering positive campaigning. This finding has significant implications for campaigning politicians and for scholars studying campaign and media effects....

  3. Spanning and independence properties of frame partitions

    CERN Document Server

    Bodmann, Bernhard G; Paulsen, Vern I; Speegle, Darrin

    2010-01-01

    We answer a number of open problems in frame theory concerning the decomposition of frames into linearly independent and/or spanning sets. We prove that in finite dimensional Hilbert spaces, Parseval frames with norms bounded away from 1 can be decomposed into a number of sets whose complements are spanning, where the number of these sets only depends on the norm bound. We also prove, assuming the Kadison-Singer conjecture is true, that this holds for infinite dimensional Hilbert spaces. Further, we prove a stronger result for Parseval frames whose norms are uniformly small, which shows that in addition to the spanning property, the sets can be chosen to be independent, and the complement of each set to contain a number of disjoint, spanning sets.

  4. Compactly supported frames for decomposition spaces

    DEFF Research Database (Denmark)

    Nielsen, Morten; Rasmussen, Kenneth Niemann

    2012-01-01

    are sufficiently close have an almost diagonal “change of frame coefficient” matrix. Finally, we approximate to an arbitrary degree an already known frame for Triebel-Lizorkin type spaces and the associated modulation spaces with a single function with sufficient decay in both direct and frequency space.......In this article we study a construction of compactly supported frame expansions for decomposition spaces of Triebel-Lizorkin type and for the associated modulation spaces. This is done by showing that finite linear combinations of shifts and dilates of a single function with sufficient decay...... in both direct and frequency space can constitute a frame for Triebel-Lizorkin type spaces and the associated modulation spaces. First, we extend the machinery of almost diagonal matrices to Triebel-Lizorkin type spaces and the associated modulation spaces. Next, we prove that two function systems which...

  5. Degradation of a quantum reference frame

    CERN Document Server

    Bartlett, S D; Spekkens, R W; Turner, P S; Bartlett, Stephen D.; Rudolph, Terry; Spekkens, Robert W.; Turner, Peter S.

    2006-01-01

    We investigate the degradation of reference frames, treated as dynamical quantum systems, and quantify their longevity as a resource for performing tasks in quantum information processing. We adopt an operational measure of a reference frame's longevity, namely, the number of measurements that can be made against it with a certain error tolerance. We investigate two distinct types of reference frame: a reference direction, realized by a spin-j system, and a phase reference, realized by an oscillator mode with bounded energy. For both cases, we show that our measure of longevity increases quadratically with the size of the reference system and is therefore non-additive. Our results quantify the extent to which microscopic or mesoscopic reference frames may be used for repeated, high-precision measurements, without needing to be reset -- a question that is important for some implementations of quantum computing. We illustrate our results using the proposed single-spin measurement scheme of magnetic resonance fo...

  6. Spatial reference frame agreement in quantum networks

    International Nuclear Information System (INIS)

    In order to communicate information in a quantum network effectively, all network nodes should share a common reference frame. Here, we propose to study how well m nodes in a quantum network can establish a common spatial reference frame from scratch, even though t of them may be arbitrarily faulty. We present a protocol that allows all correctly functioning nodes to agree on a common reference frame as long as they are fully connected and not more than tframe agreement to be lifted to a protocol for a quantum network. (paper)

  7. Vibration of x-braced portal frames

    Science.gov (United States)

    Chang, C. H.; Wang, P. Y.; Lin, Y. W.

    1987-09-01

    Both free and forced vibrations of elastic X-braced portal frames are investigated. Solutions of the Euler-Bernoulli equation for the transverse vibration coupled with the axial vibration are used. The first five natural frequencies, with the angle of inclination, α, of the bracing bars ranging from 15° to 75°, with different slenderness ratios, R, of the columns, and different stiffness of the floor beam and crossing bars, are presented along with two sets of the natural modes of the frames with α = 45°. For the forced vibration, the dynamic responses of the frames with a concentrated horizontal time dependent force acting at a top joint are studied. The responses of the frames with α = 45° are analyzed in detail.

  8. High Performance Commercial Fenestration Framing Systems

    Energy Technology Data Exchange (ETDEWEB)

    Mike Manteghi; Sneh Kumar; Joshua Early; Bhaskar Adusumalli

    2010-01-31

    A major objective of the U.S. Department of Energy is to have a zero energy commercial building by the year 2025. Windows have a major influence on the energy performance of the building envelope as they control over 55% of building energy load, and represent one important area where technologies can be developed to save energy. Aluminum framing systems are used in over 80% of commercial fenestration products (i.e. windows, curtain walls, store fronts, etc.). Aluminum framing systems are often required in commercial buildings because of their inherent good structural properties and long service life, which is required from commercial and architectural frames. At the same time, they are lightweight and durable, requiring very little maintenance, and offer design flexibility. An additional benefit of aluminum framing systems is their relatively low cost and easy manufacturability. Aluminum, being an easily recyclable material, also offers sustainable features. However, from energy efficiency point of view, aluminum frames have lower thermal performance due to the very high thermal conductivity of aluminum. Fenestration systems constructed of aluminum alloys therefore have lower performance in terms of being effective barrier to energy transfer (heat loss or gain). Despite the lower energy performance, aluminum is the choice material for commercial framing systems and dominates the commercial/architectural fenestration market because of the reasons mentioned above. In addition, there is no other cost effective and energy efficient replacement material available to take place of aluminum in the commercial/architectural market. Hence it is imperative to improve the performance of aluminum framing system to improve the energy performance of commercial fenestration system and in turn reduce the energy consumption of commercial building and achieve zero energy building by 2025. The objective of this project was to develop high performance, energy efficient commercial

  9. Digital Reading: The Transformation of Reading Practices

    OpenAIRE

    Cardoso, Gustavo; Ganito, Carla; Ferreira, Cátia

    2012-01-01

    The paper aims to present the preliminary results of a two-year project having as scope the future of the book and libraries stemming from the current reading practices in Portugal. In the scope of the project, the presentation of the findings will be focused on the mobile consumption practices in Portugal. This research is based on a mixed methodology: a quantitative survey – Network Society in Portugal – articulated with a qualitative analysis of the discourses of the representatives of wha...

  10. Stability of wavelet frames with matrix dilations

    DEFF Research Database (Denmark)

    Christensen, Ole; Sun, Wenchang

    2006-01-01

    Under certain assumptions we show that a wavelet frame {Tau(A(j), b(j,k))psi} (j,k is an element of Z) := {vertical bar detA(j)vertical bar(-1/2) psi(A(j)(-1)(x - b(j,k)))} (j,k is an element of Z) in L-2(R-d) remains a frame when the dilation matrices A(j) and the translation parameters b(j,k) are...

  11. Quantum Stackelberg duopoly in noninertial frame

    CERN Document Server

    Khan, Salman

    2011-01-01

    We study the influence of Unruh effect on quantum Stackelberg duopoly. We show that the acceleration of noninertial frame strongly effects the payoffs of the firms. The validation of subgame perfect Nash equilibrium is limited to a particular range of acceleration of the noninertial frame. The benefit of initial state entanglement in the quantum form of the duopoly is adversely affected by the acceleration. The duopoly can become as a follower advantage only in a small region of the acceleration.

  12. Physics within a quantum reference frame

    International Nuclear Information System (INIS)

    We investigate the physics of quantum reference frames. Specifically, we study several simple scenarios involving a small number of quantum particles, whereby we promote one of these particles to the role of a quantum observer and ask what is the description of the rest of the system, as seen by this observer? We highlight the interesting aspects of such questions by presenting a number of apparent paradoxes. By unravelling these paradoxes, we obtain a better understanding of the physics of quantum reference frames.

  13. Equivalence of the Einstein and Jordan frames

    OpenAIRE

    Postma, Marieke; Volponi, Marco

    2014-01-01

    No experiment can measure an absolute scale: every dimensionfull quantity has to be compared to some fixed unit scale in order to be measured, and thus only dimensionless quantities are really physical. The Einstein and Jordan frame are related by a conformal transformation of the metric, which amounts to rescaling all length scales. Since the absolute scale cannot be measured, both frames describe the same physics, and are equivalent. In this article we make this explicit by rewriting the ac...

  14. Quantum communication, reference frames and gauge theory

    OpenAIRE

    van Enk, S. J.

    2006-01-01

    We consider quantum communication in the case that the communicating parties not only do not share a reference frame but use imperfect quantum communication channels, in that each channel applies some fixed but unknown unitary rotation to each qubit. We discuss similarities and differences between reference frames within that quantum communication model and gauge fields in gauge theory. We generalize the concept of refbits and analyze various quantum communication protocols within the communi...

  15. Quantum communication, reference frames, and gauge theory

    International Nuclear Information System (INIS)

    We consider quantum communication in the case that the communicating parties not only do not share a reference frame but use imperfect quantum communication channels, in that each channel applies some fixed but unknown unitary rotation to each qubit. We discuss similarities and differences between reference frames within that quantum communication model and gauge fields in gauge theory. We generalize the concept of refbits and analyze various quantum communication protocols within the communication model

  16. Non-orthogonal fusion frames and the sparsity of fusion frame operators

    CERN Document Server

    Cahill, Jameson; Li, Shidong

    2010-01-01

    Fusion frames have become a major tool in the implementation of distributed systems. The effectiveness of fusion frame applications in distributed systems is reflected in the efficiency of the end fusion process. This in turn is reflected in the efficiency of the inversion of the fusion frame operator $S_{\\cW}$, which in turn is heavily dependent on the sparsity of $S_{\\cW}$. We will show that sparsity of the fusion frame operator naturally exists by introducing a notion of {\\it non-orthogonal fusion frames}. We show that for a fusion frame $\\{W_i,v_i\\}_{i\\in I}$, if $\\text{dim}(W_i)=k_i$, then the matrix of the non-orthogonal fusion frame operator $\\cSw$ has in its corresponding location at most a $k_i\\times k_i$ block matrix. We provide necessary and sufficient conditions for which the new fusion frame operator $\\cSw$ is diagonal and/or a multiple of an identity. A set of other critical questions are also addressed. A scheme of {\\it multiple fusion frames} whose corresponding fusion frame operator becomes a...

  17. Impact of the Reading Buddies Program on Reading Level and Attitude Towards Reading

    OpenAIRE

    Hayley Dolman; Serena Boyte-Hawryluk

    2013-01-01

    Objective – This research examines the Reading Buddies program at the Grande Prairie Public Library, which took place in July and August of 2011 and 2012. The Reading Buddies program pairs lower elementary students with teen volunteers for reading practice over the summer. The aim of the study was to discover how much impact the program would have on participating children’s reading levels and attitudes towards reading.Methods – During the first and last sessions of the Reading Buddies progra...

  18. Speakers' choice of frame in binary choice

    Directory of Open Access Journals (Sweden)

    Marc van Buiten

    2009-02-01

    Full Text Available A distinction is proposed between extit{recommending for} preferred choice options and extit{recommending against} non-preferred choice options. In binary choice, both recommendation modes are logically, though not psychologically, equivalent. We report empirical evidence showing that speakers recommending for preferred options predominantly select positive frames, which are less common when speakers recommend against non-preferred options. In addition, option attractiveness is shown to affect speakers' choice of frame, and adoption of recommendation mode. The results are interpreted in terms of three compatibility effects, (i extit{recommendation mode---valence framing compatibility}: speakers' preference for positive framing is enhanced under extit{recommending for} and diminished under extit{recommending against} instructions, (ii extit{option attractiveness---valence framing compatibility}: speakers' preference for positive framing is more pronounced for attractive than for unattractive options, and (iii extit{recommendation mode---option attractiveness compatibility}: speakers are more likely to adopt a extit{recommending for} approach for attractive than for unattractive binary choice pairs.

  19. Comparative Analysis of Frames with Varying Inertia

    Directory of Open Access Journals (Sweden)

    Prerana Nampalli

    2015-01-01

    Full Text Available This paper presents an elastic seismic response of reinforced concrete frames with 3 variations of heights, i.e. (G+2, (G+4, (G+6 storey models are compared for bare frame and frame with brick infill structures which have been analyzed for gravity as well as seismic forces and their response is studied as the geometric parameters varying from view point of predicting behavior of similar structures subjected to similar loads or load combinations. In this study, two different cases are selected i.e. frames with prismatic members and frames with non-prismatic members. The structural response of various members when geometry changes physically, as in case of linear and parabolic haunches provided beyond the face of columns at beam column joints or step variations as in case of stepped haunches was also studied. Frames have been analyzed statically as well as dynamically using ETABS-9.7.4 software referring IS: 456-2000, IS: 1893 (Part-12002 and the results so obtained are grouped into various categories

  20. Celestial Reference Frames at Multiple Radio Wavelengths

    Science.gov (United States)

    Jacobs, Christopher S.

    2012-01-01

    In 1997 the IAU adopted the International Celestial Reference Frame (ICRF) built from S/X VLBI data. In response to IAU resolutions encouraging the extension of the ICRF to additional frequency bands, VLBI frames have been made at 24, 32, and 43 gigahertz. Meanwhile, the 8.4 gigahertz work has been greatly improved with the 2009 release of the ICRF-2. This paper discusses the motivations for extending the ICRF to these higher radio bands. Results to date will be summarized including evidence that the high frequency frames are rapidly approaching the accuracy of the 8.4 gigahertz ICRF-2. We discuss current limiting errors and prospects for the future accuracy of radio reference frames. We note that comparison of multiple radio frames is characterizing the frequency dependent systematic noise floor from extended source morphology and core shift. Finally, given Gaia's potential for high accuracy optical astrometry, we have simulated the precision of a radio-optical frame tie to be approximately10-15 microarcseconds ((1-sigma) (1-standard deviation), per component).