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Sample records for alternative reading frames

  1. Value of T cell receptor gamma alternate reading frame protein and keratin 5 in endometrial carcinoma

    Institute of Scientific and Technical Information of China (English)

    ZHAO Li-jun; LI Xiao-ping; QI Wen-juan; WANG Jian-liu; WEI Li-hui

    2013-01-01

    Background Tumors with different gene expression develop and progress in different ways.To deepen our understanding of the progression in endometrial cancer,and provide a useful tool for accurate diagnosis and prognosis assessment,we identified the new molecular prognostic markers in endometrial carcinoma and analyzed the relationship of them with clinical and pathological features of endometrial carcinoma.Methods Ninety-four cases of endometrial endometrioid adenocarcinoma with complete data from the Peking University People's Hospital from 2000 to 2008 and 40 cases of normal endometrium were enrolled.Among these,30 endometrial endometrioid adenocarcinoma samples of different International Federation of Gynecology and Obstetrics (FIGO) stage were selected for further Agilent genome-wide microarray analysis.Significance analysis of microarrays (SAM) was used to identify genes that are significantly associated with tumor progress.Immunohistochemistry was utilized to identify the genes of interest in endometrial carcinoma and normal endometrium.The relationship between the genes and the age,clinical stage,histological grade,myometrium invaded depth,lymph node metastasis status,and the expression of ER,PR,P53,and PTEN were analyzed by x2 test.Results Analysis between FIGO 1988 stage Ⅰ and stage Ⅲ identified a 362-gene "progress signature"; 171 downregulated and 191 up-regulated genes.Among the alterative genes,TARP (T cell receptor gamma alternate reading frame protein) and KRT5 (keratin 5) decreased 3.57 fold and 5.8 fold in FIGO stage Ⅲ patients.The expression of TARP in endometrial carcinoma increased compared to normal endometrium,while that of KRT5 decreased (P<0.05).The expression of TARP and KRT5 decreased when stage,histological grading,myometrium invaded depth increased (P<0.05).In the cases with lymph node metastasis,the expression of TARP decreased,while the expression of KRT5 did not differ (both P<0.05) both.The expression of P53 had a

  2. Hepatitis C Virus Frameshift/Alternate Reading Frame Protein Suppresses Interferon Responses Mediated by Pattern Recognition Receptor Retinoic-Acid-Inducible Gene-I.

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    Seung Bum Park

    Full Text Available Hepatitis C virus (HCV actively evades host interferon (IFN responses but the mechanisms of how it does so are not completely understood. In this study, we present evidence for an HCV factor that contributes to the suppression of retinoic-acid-inducible gene-I (RIG-I-mediated IFN induction. Expression of frameshift/alternate reading frame protein (F/ARFP from HCV -2/+1 frame in Huh7 hepatoma cells suppressed type I IFN responses stimulated by HCV RNA pathogen-associated molecular pattern (PAMP and poly(IC. The suppression occurred independently of other HCV factors; and activation of interferon stimulated genes, TNFα, IFN-λ1, and IFN-λ2/3 was likewise suppressed by HCV F/ARFP. Point mutations in the full-length HCV sequence (JFH1 genotype 2a strain were made to introduce premature termination codons in the -2/+1 reading frame coding for F/ARFP while preserving the original reading frame, which enhanced IFNα and IFNβ induction by HCV. The potentiation of IFN response by the F/ARFP mutations was diminished in Huh7.5 cells, which already have a defective RIG-I, and by decreasing RIG-I expression in Huh7 cells. Furthermore, adding F/ARFP back via trans-complementation suppressed IFN induction in the F/ARFP mutant. The F/ARFP mutants, on the other hand, were not resistant to exogenous IFNα. Finally, HCV-infected human liver samples showed significant F/ARFP antibody reactivity, compared to HCV-uninfected control livers. Therefore, HCV F/ARFP likely cooperates with other viral factors to suppress type I and III IFN induction occurring through the RIG-I signaling pathway. This study identifies a novel mechanism of pattern recognition receptor modulation by HCV and suggests a biological function of the HCV alternate reading frame in the modulation of host innate immunity.

  3. Alternate Reading Frame Protein (F Protein) of Hepatitis C Virus: Paradoxical Effects of Activation and Apoptosis on Human Dendritic Cells Lead to Stimulation of T Cells

    Science.gov (United States)

    Samrat, Subodh Kumar; Li, Wen; Singh, Shakti; Kumar, Rakesh; Agrawal, Babita

    2014-01-01

    Hepatitis C virus (HCV) leads to chronic infection in the majority of infected individuals due to lack, failure, or inefficiency of generated adaptive immune responses. In a minority of patients, acute infection is followed by viral clearance. The immune correlates of viral clearance are not clear yet but have been extensively investigated, suggesting that multispecific and multifunctional cellular immunity is involved. The generation of cellular immunity is highly dependent upon how antigen presenting cells (APCs) process and present various viral antigens. Various structural and non-structural HCV proteins derived from the open reading frame (ORF) have been implicated in modulation of dendritic cells (DCs) and APCs. Besides the major ORF proteins, the HCV core region also encodes an alternate reading frame protein (ARFP or F), whose function in viral pathogenesis is not clear. In the current studies, we sought to determine the role of HCV-derived ARFP in modulating dendritic cells and stimulation of T cell responses. Recombinant adenovirus vectors containing F or core protein derived from HCV (genotype 1a) were prepared and used to endogenously express these proteins in dendritic cells. We made an intriguing observation that endogenous expression of F protein in human DCs leads to contrasting effects on activation and apoptosis of DCs, allowing activated DCs to efficiently internalize apoptotic DCs. These in turn result in efficient ability of DCs to process and present antigen and to prime and stimulate F protein derived peptide-specific T cells from HCV-naive individuals. Taken together, our findings suggest important aspects of F protein in modulating DC function and stimulating T cell responses in humans. PMID:24475147

  4. Alternate reading frame protein (F protein of hepatitis C virus: paradoxical effects of activation and apoptosis on human dendritic cells lead to stimulation of T cells.

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    Subodh Kumar Samrat

    Full Text Available Hepatitis C virus (HCV leads to chronic infection in the majority of infected individuals due to lack, failure, or inefficiency of generated adaptive immune responses. In a minority of patients, acute infection is followed by viral clearance. The immune correlates of viral clearance are not clear yet but have been extensively investigated, suggesting that multispecific and multifunctional cellular immunity is involved. The generation of cellular immunity is highly dependent upon how antigen presenting cells (APCs process and present various viral antigens. Various structural and non-structural HCV proteins derived from the open reading frame (ORF have been implicated in modulation of dendritic cells (DCs and APCs. Besides the major ORF proteins, the HCV core region also encodes an alternate reading frame protein (ARFP or F, whose function in viral pathogenesis is not clear. In the current studies, we sought to determine the role of HCV-derived ARFP in modulating dendritic cells and stimulation of T cell responses. Recombinant adenovirus vectors containing F or core protein derived from HCV (genotype 1a were prepared and used to endogenously express these proteins in dendritic cells. We made an intriguing observation that endogenous expression of F protein in human DCs leads to contrasting effects on activation and apoptosis of DCs, allowing activated DCs to efficiently internalize apoptotic DCs. These in turn result in efficient ability of DCs to process and present antigen and to prime and stimulate F protein derived peptide-specific T cells from HCV-naive individuals. Taken together, our findings suggest important aspects of F protein in modulating DC function and stimulating T cell responses in humans.

  5. Predicting statistical properties of open reading frames in bacterial genomes.

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    Katharina Mir

    Full Text Available An analytical model based on the statistical properties of Open Reading Frames (ORFs of eubacterial genomes such as codon composition and sequence length of all reading frames was developed. This new model predicts the average length, maximum length as well as the length distribution of the ORFs of 70 species with GC contents varying between 21% and 74%. Furthermore, the number of annotated genes is predicted with high accordance. However, the ORF length distribution in the five alternative reading frames shows interesting deviations from the predicted distribution. In particular, long ORFs appear more often than expected statistically. The unexpected depletion of stop codons in these alternative open reading frames cannot completely be explained by a biased codon usage in the +1 frame. While it is unknown if the stop codon depletion has a biological function, it could be due to a protein coding capacity of alternative ORFs exerting a selection pressure which prevents the fixation of stop codon mutations. The comparison of the analytical model with bacterial genomes, therefore, leads to a hypothesis suggesting novel gene candidates which can now be investigated in subsequent wet lab experiments.

  6. Bioprospecting open reading frames for peptide effectors.

    Science.gov (United States)

    Xiong, Ling; Scott, Charles

    2014-01-01

    Recent successes in the development of small-molecule antagonists of protein-protein interactions designed based on co-crystal structures of peptides bound to their biological targets confirm that short peptides derived from interacting proteins can be high-value ligands for pharmacologic validation of targets and for identification of druggable sites. Evolved sequence space is likely to be enriched for interacting peptides, but identifying minimal peptide effectors within genomic sequence can be labor intensive. Here we describe the use of incremental truncation to diversify genetic material on the scale of open reading frames into comprehensive libraries of constituent peptides. The approach is capable of generating peptides derived from both continuous and discontinuous sequence elements, and is compatible with the expression of free linear or backbone cyclic peptides, with peptides tethered to amino- or carboxyl-terminal fusion partners or with the expression of peptides displayed within protein scaffolds (peptide aptamers). Incremental truncation affords a valuable source of molecular diversity to interrogate the druggable genome or evaluate the therapeutic potential of candidate genes.

  7. West Nile alternative open reading frame (N-NS4B/WARF4 is produced in infected West Nile Virus (WNV cells and induces humoral response in WNV infected individuals

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    Faggioni Giovanni

    2012-11-01

    Full Text Available Abstract Background West Nile Virus (WNV is a flavivirus that requires an efficient humoral and cellular host response for the control of neuroinvasive infection. We previously reported the existence of six alternative open reading frame proteins in WNV genome, one of which entitled WARF4 is exclusively restricted to the lineage I of the virus. WARF4 is able to elicit antibodies in WNV infected horses; however, there was no direct experimental proof of the existence of this novel protein. The purpose of this study was to demonstrate the in vitro production of WARF4 protein following WNV infection of cultured VERO cells and its immunity in WNV infected individuals. Results We produced a monoclonal antibody against WARF4 protein (MAb 3A12 which detected the novel protein in WNV lineage I-infected, cultured VERO cells while it did not react with WNV lineage II infected cells. MAb 3A12 specificity to WARF4 protein was confirmed by its reactivity to only one peptide among four analyzed that cover the full WARF4 amino acids sequence. In addition, WARF4 protein was expressed in the late phase of WNV lineage I infection. Western blotting and bioinformatics analyses strongly suggest that the protein could be translated by programmed −1 ribosomal frameshifting process. Since WARF4 is embedded in the NS4B gene, we rename this novel protein N-NS4B/WARF4. Furthermore, serological analysis shows that N-NS4B/WARF4 is able to elicit antibodies in WNV infected individuals. Conclusions N-NS4B/WARF4 is the second Alternative Reading Frame (ARF protein that has been demonstrated to be produced following WNV infection and might represent a novel tool for a better characterization of immune response in WNV infected individuals. Further serological as well as functional studies are required to characterize the function of the N-NS4B/WARF4 protein. Since the virus might actually make an extensive use of ARFs, it appears important to investigate the novel six ARF

  8. [Alternatives for Reading Teacher Educator Education].

    Science.gov (United States)

    Palmatier, Robert A., Ed.

    1976-01-01

    The "Epistle" is the publication forum of Professors of Reading Teacher Educators, a special-interest group of the International Reading Association. The topic considered in this issue is alternatives or positive approaches for dealing with current concerns in graduate education in reading. In "Speculations on the Management of Retrenchment in…

  9. Open Reading Frame Phylogenetic Analysis on the Cloud

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    Che-Lun Hung

    2013-01-01

    Full Text Available Phylogenetic analysis has become essential in researching the evolutionary relationships between viruses. These relationships are depicted on phylogenetic trees, in which viruses are grouped based on sequence similarity. Viral evolutionary relationships are identified from open reading frames rather than from complete sequences. Recently, cloud computing has become popular for developing internet-based bioinformatics tools. Biocloud is an efficient, scalable, and robust bioinformatics computing service. In this paper, we propose a cloud-based open reading frame phylogenetic analysis service. The proposed service integrates the Hadoop framework, virtualization technology, and phylogenetic analysis methods to provide a high-availability, large-scale bioservice. In a case study, we analyze the phylogenetic relationships among Norovirus. Evolutionary relationships are elucidated by aligning different open reading frame sequences. The proposed platform correctly identifies the evolutionary relationships between members of Norovirus.

  10. A Probabilistic Genome-Wide Gene Reading Frame Sequence Model

    DEFF Research Database (Denmark)

    Have, Christian Theil; Mørk, Søren

    We introduce a new type of probabilistic sequence model, that model the sequential composition of reading frames of genes in a genome. Our approach extends gene finders with a model of the sequential composition of genes at the genome-level -- effectively producing a sequential genome annotation...... and are evaluated by the effect on prediction performance. Since bacterial gene finding to a large extent is a solved problem it forms an ideal proving ground for evaluating the explicit modeling of larger scale gene sequence composition of genomes. We conclude that the sequential composition of gene reading frames...... as output. The model can be used to obtain the most probable genome annotation based on a combination of i: a gene finder score of each gene candidate and ii: the sequence of the reading frames of gene candidates through a genome. The model --- as well as a higher order variant --- is developed and tested...

  11. Novel exon of mammalian ADAR2 extends open reading frame.

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    Stefan Maas

    Full Text Available BACKGROUND: The post-transcriptional processing of pre-mRNAs by RNA editing contributes significantly to the complexity of the mammalian transcriptome. RNA editing by site-selective A-to-I modification also regulates protein function through recoding of genomically specified sequences. The adenosine deaminase ADAR2 is the main enzyme responsible for recoding editing and loss of ADAR2 function in mice leads to a phenotype of epilepsy and premature death. Although A-to-I RNA editing is known to be subject to developmental and cell-type specific regulation, there is little knowledge regarding the mechanisms that regulate RNA editing in vivo. Therefore, the characterization of ADAR expression and identification of alternative ADAR variants is an important prerequisite for understanding the mechanisms for regulation of RNA editing and the causes for deregulation in disease. METHODOLOGY/PRINCIPAL FINDINGS: Here we present evidence for a new ADAR2 splice variant that extends the open reading frame of ADAR2 by 49 amino acids through the utilization of an exon located 18 kilobases upstream of the previously annotated first coding exon and driven by a candidate alternative promoter. Interestingly, the 49 amino acid extension harbors a sequence motif that is closely related to the R-domain of ADAR3 where it has been shown to function as a basic, single-stranded RNA binding domain. Quantitative expression analysis shows that expression of the novel ADAR2 splice variant is tissue specific being highest in the cerebellum. CONCLUSIONS/SIGNIFICANCE: The strong sequence conservation of the ADAR2 R-domain between human, mouse and rat ADAR2 genes suggests a conserved function for this isoform of the RNA editing enzyme.

  12. Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection

    OpenAIRE

    2015-01-01

    Viruses use alternate mechanisms to increase the coding capacity of their viral genomes. The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts an RNA structure that can direct translation in 0 and +1 reading frames to produce the viral structural proteins and an overlapping ORFx product. Here we provide structural and biochemical evidence that the PKI domain of the IRES mimics a complete tRNA-like structure to facilitate reading frame selection and allows the viral IR...

  13. Framing effects in reading: an fMRI study

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    Laura Danelli

    2014-04-01

    Anatomofunctional results (Figure 1 showed that the left occipital, the anterior and posterior temporal regions, and the left intraparietal sulcus were specifically activated when reading targets in a lexical frame. The left posterior inferior temporal and inferior parietal regions were activated in sublexical condition. Finally, reading along the two routes commonly activated the Visual Word Form Area, the premotor cortex, the left frontal areas and the left SMA, suggesting an involvement of these regions in early-input (early orthographic processing and late-output processes (phonological output buffer and articulatory programming. Conclusions: These results represent a new fine-grained description of the neurofunctional correlates of the dual route model partially supporting the recent anatomical investigations in patients with specific forms of acquired dyslexia (Ripamonti, Aggujaro, Molteni, Zonca, Ghirardi, & Luzzatti, 2014.

  14. Genome-Wide Search for Translated Upstream Open Reading Frames in Arabidopsis Thaliana.

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    Hu, Qiwen; Merchante, Catharina; Stepanova, Anna N; Alonso, Jose M; Heber, Steffen

    2016-03-01

    Upstream open reading frames (uORFs) are open reading frames that occur within the 5' UTR of an mRNA. uORFs have been found in many organisms. They play an important role in gene regulation, cell development, and in various metabolic processes. It is believed that translated uORFs reduce the translational efficiency of the main coding region. However, only few uORFs are experimentally characterized. In this paper, we use ribosome footprinting together with a semi-supervised approach based on stacking classification models to identify translated uORFs in Arabidopsis thaliana. Our approach identified 5360 potentially translated uORFs in 2051 genes. GO terms enriched in genes with translated uORFs include catalytic activity, binding, transferase activity, phosphotransferase activity, kinase activity, and transcription regulator activity. The reported uORFs occur with a higher frequency in multi-isoform genes, and some uORFs are affected by alternative transcript start sites or alternative splicing events. Association rule mining revealed sequence features associated with the translation status of the uORFs. We hypothesize that uORF translation is a complex process that might be regulated by multiple factors. The identified uORFs are available online at:https://www.dropbox.com/sh/zdutupedxafhly8/AABFsdNR5zDfiozB7B4igFcja?dl=0. This paper is the extended version of our research presented at ISBRA 2015.

  15. DNA vaccination of pigs with open reading frame 1-7 of PRRS virus

    DEFF Research Database (Denmark)

    Barfoed, Annette Malene; Blixenkrone-Møller, Merete; Jensen, Merethe Holm

    2004-01-01

    We cloned all open reading frames of a Danish isolate of porcine reproductive and respiratory syndrome (PRRS) virus in DNA vaccination vectors. Pigs were vaccinated using a gene gun with each single construct (ORF1, ORF2, ORF3, ORF4, ORF5, ORF6, or ORF7) or combinations thereof. Vaccination...

  16. Flatworms have lost the right open reading frame kinase 3 gene during evolution

    OpenAIRE

    Bert Breugelmans; Ansell, Brendan R. E.; Young, Neil D; Parisa Amani; Stroehlein, Andreas J.; Sternberg, Paul W.; Jex, Aaron R.; Boag, Peter R.; Andreas Hofmann; Gasser, Robin B

    2015-01-01

    All multicellular organisms studied to date have three ri ght o pen reading frame kinase genes (designated riok-1, riok-2 and riok-3). Current evidence indicates that riok-1 and riok-2 have essential roles in ribosome biosynthesis, and that the riok-3 gene assists this process. In the present study, we conducted a detailed bioinformatic analysis of the riok gene family in 25 parasitic flatworms (platyhelminths) for which extensive genomic and transcriptomic data sets are available. We found t...

  17. Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation

    OpenAIRE

    Joshua P Ferreira; Noderer, William L; Diaz de Arce, Alexander J; Wang, Clifford L.

    2014-01-01

    We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-tra...

  18. Eliciting preferences for alternative cancer drug treatments. The influence of framing, medium, and rater variables.

    Science.gov (United States)

    O'Connor, A M; Boyd, N F; Tritchler, D L; Kriukov, Y; Sutherland, H; Till, J E

    1985-01-01

    In oncology there is increasing interest in the development of techniques to help patients choose between alternative therapies in ways that are consistent with their preferences. The purpose of this study was to examine some methodological problems associated with the elicitation of preferences. Preferences for alternative drug therapies were sought from 208 visitors to an open house at the Ontario Cancer Institute and from 216 university nursing students. Preferences were not significantly dependent on the sex, age or professional status of the respondents, nor on the medium used for elicitation of preferences (computer terminal versus pencil-and-paper questionnaire). In contrast, the importance of the way decision problems are framed was confirmed. Comparison of a positive frame (outcomes expressed as the probability of surviving) with a negative frame (outcomes expressed as the probability of dying) and a mixed frame (probabilities of surviving and dying were both given) pointed to the presence or absence of the word "survive" in the outcome description as the main source of framing bias. The framing effect was in the opposite direction to that hypothesized.

  19. Protection against heartwater by DNA immunisation with four Ehrlichia ruminantium open reading frames.

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    Pretorius, A; Collins, N E; Steyn, H C; van Strijp, F; van Kleef, M; Allsopp, B A

    2007-03-08

    We have reported previously that a recombinant DNA vaccine consisting of four Ehrlichia ruminantium (Welgevonden) open reading frames (ORFs) known as the 1H12 cocktail provided protection against a virulent E. ruminantium (Welgevonden) needle challenge in sheep. In this study, we have investigated the vaccine effectiveness of two other cocktails of E. ruminantium (Welgevonden) ORFs, as well as single ORFs from the 1H12 cocktail, to protect sheep against a virulent needle challenge with the homologous strain. Each individual 1H12 ORF provided protection, but all the animals vaccinated with the other cocktails succumbed to the challenge.

  20. Engineering ribosomal leaky scanning and upstream open reading frames for precise control of protein translation

    Science.gov (United States)

    Ferreira, Joshua P; Noderer, William L; Diaz de Arce, Alexander J; Wang, Clifford L

    2014-01-01

    We have employed upstream open reading frames (uORFs) to systematically tune the translation levels of recombinant proteins. We present the design principles that guided the development of this technology and provide information that may help others in implementing synthetic uORFs for their own applications. We also report on recent applications to our own research projects, including the coupling of uORF and translation initiation site (TIS) engineering with small molecule-inducible post-translational control. Finally, we discuss opportunities to investigate and potentially engineer gene-specific translational responses to cellular stress. PMID:24637490

  1. A read-out system for the Medipix2 chip capable of 500 frames per second

    Energy Technology Data Exchange (ETDEWEB)

    Maiorino, M. [IFAE Institut de Fisica d' Altes Energies, UAB Campus, 08193 Barcelona (Spain)]. E-mail: maiorino@ifae.es; Martinez, R. [Centro Nacional de Microelectronica, IMB-CNM-CSIC, UAB Campus, 08193 Barcelona (Spain); Pellegrini, G. [Centro Nacional de Microelectronica, IMB-CNM-CSIC, UAB Campus, 08193 Barcelona (Spain); Blanchot, G. [IFAE Institut de Fisica d' Altes Energies, UAB Campus, 08193 Barcelona (Spain); Chmeissani, M. [IFAE Institut de Fisica d' Altes Energies, UAB Campus, 08193 Barcelona (Spain); Garcia, J. [IFAE Institut de Fisica d' Altes Energies, UAB Campus, 08193 Barcelona (Spain); Lozano, M. [Centro Nacional de Microelectronica, IMB-CNM-CSIC, UAB Campus, 08193 Barcelona (Spain); Puigdengoles, C. [IFAE Institut de Fisica d' Altes Energies, UAB Campus, 08193 Barcelona (Spain); Ullan, M. [Centro Nacional de Microelectronica, IMB-CNM-CSIC, UAB Campus, 08193 Barcelona (Spain)

    2006-07-01

    High-speed X-ray-imaging acquisition technique is a growing field that can be used to understand microscopic mechanism of different phenomena in biology and material science. IFAE and CNM developed a very high-speed readout system, named DEMAS, for the Medipix2. The system is able to read a single Medipix2 chip through the parallel bus at a rate of 1 kHz.With a duty cycle of 50%, the real sampling speed is 500 frames per second (fps). This implies that 1 ms is allocated to the exposure time and another millisecond is devoted to the read-out of the chip. In such configuration, the raw data throughput is about 500 Mbit/s. For the first time we present examples of acquisition at 500 fps of moving samples with X-rays working in direct capture and photon counting mode.

  2. The upstream open reading frame of cyclin-dependent kinase inhibitor 1A mRNA negatively regulates translation of the downstream main open reading frame

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kyoung Mi; Cho, Hana [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of); Kim, Yoon Ki, E-mail: yk-kim@korea.ac.kr [School of Life Sciences and Biotechnology, Korea University, Seoul 136-701 (Korea, Republic of)

    2012-08-03

    Highlights: Black-Right-Pointing-Pointer CDKN1A mRNA is a bona fide NMD substrate. Black-Right-Pointing-Pointer The uORF of CDKN1A mRNA is efficiently translated. Black-Right-Pointing-Pointer Translation of downstream main ORF is negatively regulated by translation of uORF in CDKN1A mRNA. -- Abstract: The first round of translation occurs on mRNAs bound by nuclear cap-binding complex (CBC), which is composed of nuclear cap-binding protein 80 and 20 (CBP80/20). During this round of translation, aberrant mRNAs are recognized and downregulated in abundance by nonsense-mediated mRNA decay (NMD), which is one of the mRNA quality control mechanisms. Here, our microarray analysis reveals that the level of cyclin-dependent kinase inhibitor 1A (CDKN1A; also known as Waf1/p21) mRNAs increases in cells depleted of cellular NMD factors. Intriguingly, CDKN1A mRNA contains an upstream open reading frame (uORF), which is a NMD-inducing feature. Using chimeric reporter constructs, we find that the uORF of CDKN1A mRNA negatively modulates translation of the main downstream ORF. These findings provide biological insights into the possible role of NMD in diverse biological pathways mediated by CDKN1A.

  3. Supplementary Reading Instruction in Alternative High Schools: A Statewide Survey of Educator Reported Practices and Barriers

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    Wilkerson, Kimber L.; Yan, Min-Chi; Perzigian, Aaron B.; Cakiroglu, Orhan

    2016-01-01

    Recent data suggest that a majority of secondary students read below the level considered proficient on state standardized tests of reading. Alternative high schools, in particular, serve a high proportion of struggling readers. This survey study investigated reading instruction provided to struggling readers in alternative schools in one state by…

  4. Insights into the translational regulation of biologically active open reading frames of Pelargonium line pattern virus.

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    Castaño, Aurora; Ruiz, Leticia; Hernández, Carmen

    2009-04-10

    Pelargonium line pattern virus (PLPV), a proposed member of a prospective genus (Pelarspovirus) within family Tombusviridae, has a positive-sense, single-stranded genomic RNA. According to previous predictions, it contains six open reading frames (ORFs) potentially encoding proteins of 27 (p27), 13 (p13), 87 (p87), 7 (p7), 6 (p6), and 37 kDa (p37). Using a variety of techniques we demonstrate that all predicted ORFs are functional, with the exception of (p13) and (p6). We also characterize a previously unidentified ORF which encodes a 9.7 kDa protein (p9.7) that is essential for viral movement. Furthermore, we present evidence that the single subgenomic RNA (sgRNA) produced by the virus directs synthesis of p7, p9.7 and p37. Remarkably, the translation of these totally unrelated proteins is coordinated via leaky-scanning. This mechanism seems to be favoured by the poor translation context of the start codon of ORF(p7), the non-AUG weak initiation codon of ORF(p9.7) and the lack of additional AUG codons in any reading frame preceding ORF(p37). The results also suggest that precise regulation of protein production from the sgRNA is critical for virus viability. Altogether, the data supports the notion that PLPV belongs to a new genus of plant viruses.

  5. An upstream open reading frame is essential for feedback regulation of ascorbate biosynthesis in Arabidopsis.

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    Laing, William A; Martínez-Sánchez, Marcela; Wright, Michele A; Bulley, Sean M; Brewster, Di; Dare, Andrew P; Rassam, Maysoon; Wang, Daisy; Storey, Roy; Macknight, Richard C; Hellens, Roger P

    2015-03-01

    Ascorbate (vitamin C) is an essential antioxidant and enzyme cofactor in both plants and animals. Ascorbate concentration is tightly regulated in plants, partly to respond to stress. Here, we demonstrate that ascorbate concentrations are determined via the posttranscriptional repression of GDP-l-galactose phosphorylase (GGP), a major control enzyme in the ascorbate biosynthesis pathway. This regulation requires a cis-acting upstream open reading frame (uORF) that represses the translation of the downstream GGP open reading frame under high ascorbate concentration. Disruption of this uORF stops the ascorbate feedback regulation of translation and results in increased ascorbate concentrations in leaves. The uORF is predicted to initiate at a noncanonical codon (ACG rather than AUG) and encode a 60- to 65-residue peptide. Analysis of ribosome protection data from Arabidopsis thaliana showed colocation of high levels of ribosomes with both the uORF and the main coding sequence of GGP. Together, our data indicate that the noncanonical uORF is translated and encodes a peptide that functions in the ascorbate inhibition of translation. This posttranslational regulation of ascorbate is likely an ancient mechanism of control as the uORF is conserved in GGP genes from mosses to angiosperms.

  6. Escherichia coli gpt gene provides dominant selection for vaccinia virus open reading frame expression vectors.

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    Falkner, F G; Moss, B

    1988-06-01

    Mycophenolic acid, an inhibitor of purine metabolism, was shown to block the replication of vaccinia virus in normal cell lines. This observation led to the development of a dominant one-step plaque selection system, based on expression of the Escherichia coli gpt gene, for the isolation of recombinant vaccinia viruses. Synthesis of xanthine-guanine phosphoribosyltransferase enabled only the recombinant viruses to form large plaques in a selective medium containing mycophenolic acid, xanthine, and hypoxanthine. To utilize the selection system efficiently, we constructed a series of plasmids that contain the E. coli gpt gene and allow insertion of foreign genes into multiple unique restriction endonuclease sites in all three reading frames between the translation initiation codon of a strong late promoter and synthetic translation termination sequences. The selection-expression cassette is flanked by vaccinia virus DNA that directs homologous recombination into the virus genome. The new vectors allow high-level expression of complete or partial open reading frames and rapid construction of recombinant viruses by facilitating the cloning steps and by simplifying their isolation. The system was tested by cloning the E. coli beta-galactosidase gene; in 24 h, this enzyme accounted for approximately 3.5% of the total infected-cell protein.

  7. Round Robin Reading: Considering Alternative Instructional Practices That Make More Sense.

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    Kelly, Patricia R.

    1995-01-01

    Discusses how preservice teachers in a reading methods course investigated the prevalence of round-robin reading in 72 elementary classrooms. Finds that the majority of the teachers used round-robin reading. Notes that the preservice teachers became acutely aware of what really goes on during round robin reading. Discusses five alternatives to…

  8. MCP detector read out with a bare quad Timepix at kilohertz frame rates

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    Vallerga, J.; Raffanti, R.; Tremsin, A.; McPhate, J.; Siegmund, O.

    2011-01-01

    The existing Berkeley neutron sensitive MCP/Timepix hybrid detector has been very successful at demonstrating energy resolved spatial imaging with a single Timepix ASIC read out at a ~ 30 Hz frame rate where each neutron's position and time (energy) is determined (X,Y,E). By increasing the detector format using a quad arrangement of Timepix readouts and increasing the frame rate to 1 kHz, we can increase our total event throughput by a factor of 120, thereby taking full advantage of the high fluxes of modern pulsed neutron sources (106 n cm-2 s-1). The key to this conversion is a new design for the ASIC readout, called the Berkeley Quad Timepix detector, consisting of 3 major subsystems. The first is a quad (2 × 2) bare Timepix ASIC board mounted directly behind the neutron sensitive MCPs in a hermetic vacuum enclosure with a sapphire window. The data from the Timepix ASICs flow to the second subsystem called the Interface board whose field programmable gate array (FPGA) rearranges and converts the digital bit stream to LVDS logic levels before sending downstream to the third subsystem, the Roach board. The Roach board is also FPGA based, and takes the data from all the ASICs and analyses the frames to extract information on the input events to pass on to the host PC. This paper describes in detail the hardware and firmware designs to accomplish this task.

  9. Tying Down Loose Ends in the Chlamydomonas Genome: Functional Significance of Abundant Upstream Open Reading Frames

    Directory of Open Access Journals (Sweden)

    Frederick R. Cross

    2016-02-01

    Full Text Available The Chlamydomonas genome has been sequenced, assembled, and annotated to produce a rich resource for genetics and molecular biology in this well-studied model organism. The annotated genome is very rich in open reading frames upstream of the annotated coding sequence (‘uORFs’: almost three quarters of the assigned transcripts have at least one uORF, and frequently more than one. This is problematic with respect to the standard ‘scanning’ model for eukaryotic translation initiation. These uORFs can be grouped into three classes: class 1, initiating in-frame with the coding sequence (CDS (thus providing a potential in-frame N-terminal extension; class 2, initiating in the 5′ untranslated sequences (5UT and terminating out-of-frame in the CDS; and class 3, initiating and terminating within the 5UT. Multiple bioinformatics criteria (including analysis of Kozak consensus sequence agreement and BLASTP comparisons to the closely related Volvox genome, and statistical comparison to cds and to random sequence controls indicate that of ∼4000 class 1 uORFs, approximately half are likely in vivo translation initiation sites. The proposed resulting N-terminal extensions in many cases will sharply alter the predicted biochemical properties of the encoded proteins. These results suggest significant modifications in ∼2000 of the ∼20,000 transcript models with respect to translation initiation and encoded peptides. In contrast, class 2 uORFs may be subject to purifying selection, and the existent ones (surviving selection are likely inefficiently translated. Class 3 uORFs are found in more than half of transcripts, frequently multiple times per transcript; however, they are remarkably similar to random sequence expectations with respect to size, number, and composition, and therefore may in most cases be selectively neutral.

  10. Global shape mimicry of tRNA within a viral internal ribosome entry site mediates translational reading frame selection.

    Science.gov (United States)

    Au, Hilda H; Cornilescu, Gabriel; Mouzakis, Kathryn D; Ren, Qian; Burke, Jordan E; Lee, Seonghoon; Butcher, Samuel E; Jan, Eric

    2015-11-24

    The dicistrovirus intergenic region internal ribosome entry site (IRES) adopts a triple-pseudoknotted RNA structure and occupies the core ribosomal E, P, and A sites to directly recruit the ribosome and initiate translation at a non-AUG codon. A subset of dicistrovirus IRESs directs translation in the 0 and +1 frames to produce the viral structural proteins and a +1 overlapping open reading frame called ORFx, respectively. Here we show that specific mutations of two unpaired adenosines located at the core of the three-helical junction of the honey bee dicistrovirus Israeli acute paralysis virus (IAPV) IRES PKI domain can uncouple 0 and +1 frame translation, suggesting that the structure adopts distinct conformations that contribute to 0 or +1 frame translation. Using a reconstituted translation system, we show that ribosomes assembled on mutant IRESs that direct exclusive 0 or +1 frame translation lack reading frame fidelity. Finally, a nuclear magnetic resonance/small-angle X-ray scattering hybrid approach reveals that the PKI domain of the IAPV IRES adopts an RNA structure that resembles a complete tRNA. The tRNA shape-mimicry enables the viral IRES to gain access to the ribosome tRNA-binding sites and form intermolecular contacts with the ribosome that are necessary for initiating IRES translation in a specific reading frame.

  11. Surveying immigrants without sampling frames - evaluating the success of alternative field methods.

    Science.gov (United States)

    Reichel, David; Morales, Laura

    2017-01-01

    This paper evaluates the sampling methods of an international survey, the Immigrant Citizens Survey, which aimed at surveying immigrants from outside the European Union (EU) in 15 cities in seven EU countries. In five countries, no sample frame was available for the target population. Consequently, alternative ways to obtain a representative sample had to be found. In three countries 'location sampling' was employed, while in two countries traditional methods were used with adaptations to reach the target population. The paper assesses the main methodological challenges of carrying out a survey among a group of immigrants for whom no sampling frame exists. The samples of the survey in these five countries are compared to results of official statistics in order to assess the accuracy of the samples obtained through the different sampling methods. It can be shown that alternative sampling methods can provide meaningful results in terms of core demographic characteristics although some estimates differ to some extent from the census results.

  12. An Alternative to Mapping a Word onto a Concept in Language Acquisition: Pragmatic Frames.

    Science.gov (United States)

    Rohlfing, Katharina J; Wrede, Britta; Vollmer, Anna-Lisa; Oudeyer, Pierre-Yves

    2016-01-01

    The classic mapping metaphor posits that children learn a word by mapping it onto a concept of an object or event. However, we believe that a mapping metaphor cannot account for word learning, because even though children focus attention on objects, they do not necessarily remember the connection between the word and the referent unless it is framed pragmatically, that is, within a task. Our theoretical paper proposes an alternative mechanism for word learning. Our main premise is that word learning occurs as children accomplish a goal in cooperation with a partner. We follow Bruner's (1983) idea and further specify pragmatic frames as the learning units that drive language acquisition and cognitive development. These units consist of a sequence of actions and verbal behaviors that are co-constructed with a partner to achieve a joint goal. We elaborate on this alternative, offer some initial parametrizations of the concept, and embed it in current language learning approaches.

  13. An Alternative to Mapping a Word onto a Concept in Language Acquisition: Pragmatic Frames

    Science.gov (United States)

    Rohlfing, Katharina J.; Wrede, Britta; Vollmer, Anna-Lisa; Oudeyer, Pierre-Yves

    2016-01-01

    The classic mapping metaphor posits that children learn a word by mapping it onto a concept of an object or event. However, we believe that a mapping metaphor cannot account for word learning, because even though children focus attention on objects, they do not necessarily remember the connection between the word and the referent unless it is framed pragmatically, that is, within a task. Our theoretical paper proposes an alternative mechanism for word learning. Our main premise is that word learning occurs as children accomplish a goal in cooperation with a partner. We follow Bruner’s (1983) idea and further specify pragmatic frames as the learning units that drive language acquisition and cognitive development. These units consist of a sequence of actions and verbal behaviors that are co-constructed with a partner to achieve a joint goal. We elaborate on this alternative, offer some initial parametrizations of the concept, and embed it in current language learning approaches. PMID:27148105

  14. Minimum probe length for unique identification of all open reading frames in a microbial genome

    Energy Technology Data Exchange (ETDEWEB)

    Sokhansanj, B A; Ng, J; Fitch, J P

    2000-03-05

    In this paper, we determine the minimum hybridization probe length to uniquely identify at least 95% of the open reading frame (ORF) in an organism. We analyze the whole genome sequences of 17 species, 11 bacteria, 4 archaea, and 2 eukaryotes. We also present a mathematical model for minimum probe length based on assuming that all ORFs are random, of constant length, and contain an equal distribution of bases. The model accurately predicts the minimum probe length for all species, but it incorrectly predicts that all ORFs may be uniquely identified. However, a probe length of just 9 bases is adequate to identify over 95% of the ORFs for all 15 prokaryotic species we studied. Using a minimum probe length, while accepting that some ORFs may not be identified and that data will be lost due to hybridization error, may result in significant savings in microarray and oligonucleotide probe design.

  15. How strategic dynamics complicate the framing of alternatives in strategic environmental assessment

    DEFF Research Database (Denmark)

    Lyhne, Ivar

    2012-01-01

    of the Danish Natural Gas Security of Supply Plan. Special emphasis is given to the framing of alternatives in the SEA process, since alternatives are directly related to the contextual developments. Based on a participative approach, strategic dynamics are mapped and the reactions and concerns in the SEA team......Unpredictable and complex developments challenge the application of strategic environmental assessment (SEA), e.g. in terms of timing, prediction, and relevance of assessments. Especially multi-actor and unstructured strategic level decision-making processes often seem to be characterised...... are outlined. The contribution of the article is enhanced empirical understanding of strategic dynamics and how it challenges SEA application....

  16. Framing effects reveal discrete lexical-semantic and sublexical procedures in reading: an fMRI study.

    Science.gov (United States)

    Danelli, Laura; Marelli, Marco; Berlingeri, Manuela; Tettamanti, Marco; Sberna, Maurizio; Paulesu, Eraldo; Luzzatti, Claudio

    2015-01-01

    According to the dual-route model, a printed string of letters can be processed by either a grapheme-to-phoneme conversion (GPC) route or a lexical-semantic route. Although meta-analyses of the imaging literature support the existence of distinct but interacting reading procedures, individual neuroimaging studies that explored neural correlates of reading yielded inconclusive results. We used a list-manipulation paradigm to provide a fresh empirical look at this issue and to isolate specific areas that underlie the two reading procedures. In a lexical condition, we embedded disyllabic Italian words (target stimuli) in lists of either loanwords or trisyllabic Italian words with unpredictable stress position. In a GPC condition, similar target stimuli were included within lists of pseudowords. The procedure was designed to induce participants to emphasize either the lexical-semantic or the GPC reading procedure, while controlling for possible linguistic confounds and keeping the reading task requirements stable across the two conditions. Thirty-three adults participated in the behavioral study, and 20 further adult participants were included in the fMRI study. At the behavioral level, we found sizeable effects of the framing manipulations that included slower voice onset times for stimuli in the pseudoword frames. At the functional anatomical level, the occipital and temporal regions, and the intraparietal sulcus were specifically activated when subjects were reading target words in a lexical frame. The inferior parietal and anterior fusiform cortex were specifically activated in the GPC condition. These patterns of activation represented a valid classifying model of fMRI images associated with target reading in both frames in the multi-voxel pattern analyses. Further activations were shared by the two procedures in the occipital and inferior parietal areas, in the premotor cortex, in the frontal regions and the left supplementary motor area. These regions are most

  17. Framing effects reveal discrete lexical-semantic and sublexical procedures in reading: an fMRI study

    Directory of Open Access Journals (Sweden)

    Laura eDanelli

    2015-09-01

    Full Text Available According to the dual-route model, a printed string of letters can be processed by either a grapheme-to-phoneme conversion (GPC route or a lexical-semantic route. Although meta-analyses of the imaging literature support the existence of distinct but interacting reading procedures, individual neuroimaging studies that explored neural correlates of reading yielded inconclusive results. We used a list-manipulation paradigm to provide a fresh empirical look at this issue and to isolate specific areas that underlie the two reading procedures. In a lexical condition, we embedded disyllabic Italian words (target stimuli in lists of either loanwords or trisyllabic Italian words with an unpredictable stress position. In a GPC condition, similar target stimuli were mixed into lists of pseudowords. The procedure was designed to induce participants to emphasize a lexical-semantic or a GPC reading procedure, while controlling for possible linguistic confounds and keeping the reading task requirements stable across the two conditions. Thirty-three adults participated in the behavioral study, and 20 other adult participants were included in the fMRI study. At the behavioral level, we found sizeable effects of the framing manipulations that included slower voice onset times for stimuli in the pseudoword frames. At the functional anatomical level, the occipital and temporal regions, and the intraparietal sulcus were specifically activated when subjects were reading target words in a lexical frame. The inferior parietal and anterior fusiform cortex was specifically activated in the GPC condition. These patterns of activation represented a valid classifying model of fMRI images associated with target reading in both frames in the multi-voxel pattern analyses. Further activations were shared by the two procedures in the occipital and inferior parietal areas, in the premotor cortex, in the frontal regions and the left supplementary motor area. These regions are most

  18. HIGH VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL150 OPEN READING FRAME IN LOW-PASSAGED CLINICAL ISOLATES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Objective To investigate the polymorphism of human cytomegalovirus (HCMV) UL150 open reading frame(ORF) in low-passaged clinical isolates, and to study the relationship between the polymorphism and different pathogenesis of congenital HCMV infection.Methods PCR was performed to amplify the entire HCMV UL150 ORF region of 29 clinical isolates, which hadbeen proven containing detectable HCMV-DNA using fluorescence quantitative PCR. PCR amplifcation products weresequenced directly, and the data were analyzed.Results Totally 25 among 29 isolates were amplified, and 18 isolates were sequenced successfully. HCMVUL150 ORF sequences derived from congenitally infected infants were high variability. The UL150 ORF in all 18 clinical isolates shifted backward by 8 nucleotides leading to frame-shift, and contained a single nucleotide deletion at nucleotide position 226 compared with that of Toledo strain. The nucleotide diversity was 0. 1% to 6. 8% and the amino acid diversity was 0. 2% to 19. 2% related to Toledo strain. However, the nucleotide diversity was 0. 1% to 6.4% and amino acid diversity was 0. 2% to 8.3% by compared with Merlin strain. Compared with Toledo, 4 new cysteine residues and 13 additional posttranslational modification sites were observed in UL150 putative proteins of clinical isolates. Moreover, the UL150 putative protein contained an additional transmembrane helix at position of 4-17 amino acid related to Toledo.Conclusion HCMV UL150 ORF and deduced amino acid sequences of clinical strains are hypervariability. No obvious linkage between the polymorphism and different pathogenesis of congenital HCMV infection is found.

  19. SEQUENCE VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL144 OPEN READING FRAME IN LOW-PASSAGE CLINICAL ISOLATES

    Institute of Scientific and Technical Information of China (English)

    Rong He; Yao-hua Ji; Qiang Ruan; Chang Xia; Lan-qing Liu; Sheng-min Lü; Ying Lu; Ying Qi; Yan-ping Ma; Qing Liu

    2004-01-01

    Objective To explore the relationship between human cytomegalovirus (HCMV) UL144 sequence variability and clinical disease.Methods HCMV UL144 open reading frame (ORF) was amplified by PCR assay in 72 lowpassage isolates [65 congenitally infective children and 7 healthy children who were HCMV-DNA positive by quantitative PCR (qPCR)]. All positive PCR products were analyzed by heteroduplex mobility assay and single-stranded conformation polymorphism (HMA-SSCP) and 32 of them were sequenced.Resuits Fifty-five patient isolates and five healthy children isolates were HCMV-UL144 positive by PCR. Sequencing and HMA-SSCP analysis showed that significant strain-specific variability was present in the UL144 ORF. Phylogenetic analysis indicated that the nucleotide sequences could be separated into 3 major genotypes. Comparing between UL144 sequences and the corresponding symptoms showed that genotype 2 did not exist in megacolon isolates. And genotype 1 and 3 were the major types among microcephaly and jaundice isolates respectively.Conclusions HCMV-UL144 existed in most of low passage isolates and sequences were hypervariable. The UL144ORF and its predicted product with the high level of sequence variability in different kinds of isolates suggest that UL144ORF might play a role in HCMV infectivity and subsequent diseases.

  20. Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames.

    Science.gov (United States)

    Liang, Xue-Hai; Shen, Wen; Sun, Hong; Migawa, Michael T; Vickers, Timothy A; Crooke, Stanley T

    2016-08-01

    Increasing the levels of therapeutic proteins in vivo remains challenging. Antisense oligonucleotides (ASOs) are often used to downregulate gene expression or to modify RNA splicing, but antisense technology has not previously been used to directly increase the production of selected proteins. Here we used a class of modified ASOs that bind to mRNA sequences in upstream open reading frames (uORFs) to specifically increase the amounts of protein translated from a downstream primary ORF (pORF). Using ASO treatment, we increased the amount of proteins expressed from four genes by 30-150% in a dose-dependent manner in both human and mouse cells. Notably, systemic treatment of mice with ASO resulted in an ∼80% protein increase of LRPPRC. The ASO-mediated increase in protein expression was sequence-specific, occurred at the level of translation and was dependent on helicase activity. We also found that the type of RNA modification and the position of modified nucleotides in ASOs affected translation of a pORF. ASOs are a useful class of therapeutic agents with broad utility.

  1. Flatworms have lost the right open reading frame kinase 3 gene during evolution.

    Science.gov (United States)

    Breugelmans, Bert; Ansell, Brendan R E; Young, Neil D; Amani, Parisa; Stroehlein, Andreas J; Sternberg, Paul W; Jex, Aaron R; Boag, Peter R; Hofmann, Andreas; Gasser, Robin B

    2015-05-15

    All multicellular organisms studied to date have three right open reading frame kinase genes (designated riok-1, riok-2 and riok-3). Current evidence indicates that riok-1 and riok-2 have essential roles in ribosome biosynthesis, and that the riok-3 gene assists this process. In the present study, we conducted a detailed bioinformatic analysis of the riok gene family in 25 parasitic flatworms (platyhelminths) for which extensive genomic and transcriptomic data sets are available. We found that none of the flatworms studied have a riok-3 gene, which is unprecedented for multicellular organisms. We propose that, unlike in other eukaryotes, the loss of RIOK-3 from flatworms does not result in an evolutionary disadvantage due to the unique biology and physiology of this phylum. We show that the loss of RIOK-3 coincides with a loss of particular proteins associated with essential cellular pathways linked to cell growth and apoptosis. These findings indicate multiple, key regulatory functions of RIOK-3 in other metazoan species. Taking advantage of a known partial crystal structure of human RIOK-1, molecular modelling revealed variability in nucleotide binding sites between flatworm and human RIOK proteins.

  2. Gene expression regulation by upstream open reading frames and human disease.

    Directory of Open Access Journals (Sweden)

    Cristina Barbosa

    Full Text Available Upstream open reading frames (uORFs are major gene expression regulatory elements. In many eukaryotic mRNAs, one or more uORFs precede the initiation codon of the main coding region. Indeed, several studies have revealed that almost half of human transcripts present uORFs. Very interesting examples have shown that these uORFs can impact gene expression of the downstream main ORF by triggering mRNA decay or by regulating translation. Also, evidence from recent genetic and bioinformatic studies implicates disturbed uORF-mediated translational control in the etiology of many human diseases, including malignancies, metabolic or neurologic disorders, and inherited syndromes. In this review, we will briefly present the mechanisms through which uORFs regulate gene expression and how they can impact on the organism's response to different cell stress conditions. Then, we will emphasize the importance of these structures by illustrating, with specific examples, how disturbed uORF-mediated translational control can be involved in the etiology of human diseases, giving special importance to genotype-phenotype correlations. Identifying and studying more cases of uORF-altering mutations will help us to understand and establish genotype-phenotype associations, leading to advancements in diagnosis, prognosis, and treatment of many human disorders.

  3. The composition of upstream open reading frames (uORF) in four genes from Trypanosoma cruzi typical strains.

    Science.gov (United States)

    Jaeger, Lauren Hubert; Brandão, Adeilton

    2011-10-01

    Upstream open reading frames (uORF) are small open reading frames located in the 5' untranslated region (5' utr) of a mature mRNA. We analysed in four strains representing the Trypanosoma cruzi groups Tc I, Tc II, Tc IV and Tc VI the uORF present in 5' utr sequences of four genes: P-type H+-ATPase 1, DEAD/H RNA helicase, casein kinase 1.1 and ferredoxin-NADP+ reductase. A segment in the 5' utr at each of these genes encompassing one or more uORF was PCR amplified and sequenced. An analysis of these sequences reveals that the uORF in T. cruzi show minor variations; however, these nucleotide substitutions mirror the divergence of T. cruzi strains into major groups.

  4. Deletion of Fifteen Open Reading Frames from Modified Vaccinia Virus Ankara Fails to Improve Immunogenicity.

    Directory of Open Access Journals (Sweden)

    Naif Khalaf Alharbi

    Full Text Available Modified vaccinia virus Ankara (MVA is a highly attenuated strain of vaccinia virus, which has been used as a recombinant vaccine vector in many vaccine development programmes. The loss of many immunosuppressive and host-range genes resulted in a safe and immunogenic vaccine vector. However it still retains some immunomodulatory genes that may reduce MVA immunogenicity. Earlier reports demonstrated that the deletion of the A41L, B15R, C6L, or C12L open reading frames (ORFs enhanced cellular immune responses in recombinant MVA (rMVA by up to 2-fold. However, previously, we showed that deletion of the C12L, A44L, A46R, B7R, or B15R ORFs from rMVA, using MVA-BAC recombineering technology, did not enhance rMVA immunogenicity at either peak or memory cellular immune responses. Here, we extend our previous study to examine the effect of deleting clusters of genes on rMVA cellular immunogenicity. Two clusters of fifteen genes were deleted in one rMVA mutant that encodes either the 85A antigen of Mycobacterium tuberculosis or an immunodominant H2-Kd-restricted murine malaria epitope (pb9. The deletion mutants were tested in prime only or prime and boost vaccination regimens. The responses showed no improved peak or memory CD8+ T cell frequencies. Our results suggest that the reported small increases in MVA deletion mutants could not be replicated with different antigens, or epitopes. Therefore, the gene deletion strategy may not be taken as a generic approach for improving the immunogenicity of MVA-based vaccines, and should be carefully assessed for every individual recombinant antigen.

  5. Genetic characterization of complete open reading frame of glycoprotein C gene of bovine herpesvirus 1

    Directory of Open Access Journals (Sweden)

    Saurabh Majumder

    2013-10-01

    Full Text Available Aim: To characterize one of the major glycoprotein genes viz., glycoprotein C (gC; UL44, unique long region 44 of bovineherpesvirus 1(BoHV1 of Indian origin at genetic and phylogenetic level.Materials and Methods: A bovine herpesvirus 1 isolate viz., (BoHV1/IBR 216 II/ 1976/ India maintained at Division ofVirology, IVRI, Mukteswar was used for the current study. The DNA was extracted using commercial kit and the completeORF of gC gene was amplified, cloned, and sequenced by conventional Sanger sequencing method. The sequence wasgenetically and phylogenetically analysed using various bioinformatic tools. The sequence was submitted in the Genbankwith accession number Kc756965.Results: The complete ORF of gC gene was amplified and sequenced. It showed 100% sequence homology with referencecooper strain of BoHV1 and divergence varied from 0% to 2.7% with other isolates of BoHV1. The isolate under study haddivergence of 9.2%, 13%, 26.6%, and 9.2% with BoHV5 (Bovine herpesvirus 5, CvHV1 (Cervid herpesvirus 1, CpHV1(Caprine herpesvirus 1, and BuHV1 (Bubaline herpesvirus 1, respectively.Conclusion: This is the first genetic characterization of complete open reading frame (ORF of glycoprotein C gene (UL44 ofIndian isolate of BoHV1. The gC gene of BoHV1 is highly conserved among all BoHV1 isolates and it can be used as a targetfor designing diagnostic primers for the specific detection of BoHV1.

  6. Reading Fluency through Alternative Text: Rereading with an Interact Sing-to-Read Program Embedded within a Middle School Music Classroom

    Science.gov (United States)

    Biggs, Marie C.; Watkins, Nancy A.

    2008-01-01

    Singing exaggerates the language of reading. The students find their voices in the rhythm and bounce of language by using music as an alternative technological approach to reading. A concurrent mixed methods study was conducted to investigate the use of an interactive sing-to-read program Tune Into Reading (Electronic Learning Products, 2006)…

  7. Communicative Approach: An Alternative Method Used in Improving Students' Academic Reading Achievement

    Science.gov (United States)

    Irmawati, Noer Doddy

    2012-01-01

    Academic reading is a difficult subject to be mastered. It is needed because most of books or references are written in English. The emphasis is on academic reading which becomes a compulsory subject that must be taught and understood in Faculty of Letters UAD Yogyakarta. Communicative approach is used and applied as an alternative method in the…

  8. Random Access Frames (RAF): Alternative to Rack and Standoff for Deep Space Habitat Outfitting

    Science.gov (United States)

    Howe, A. Scott; Polit-Casillas, Raul

    2014-01-01

    A modular Random Access Frame (RAF) system is proposed as an alternative to the International Standard Payload Rack (ISPR) for internal module layout and outfitting in a Deep Space Habitat (DSH). The ISPR approach was designed to allow for efficient interchangeability of payload and experiments for the International Space Station (ISS) when frequent resupply missions were available (particularly the now-retired Space Shuttle). Though the standard interface approach to the ISPR system allowed integration of subsystems and hardware from a variety of sources and manufacturers, the heavy rack and standoff approach may not be appropriate when resupply or swap-out capabilities are not available, such as on deep space, long-duration missions. The lightweight RAF concept can allow a more dense packing of stowage and equipment, and may be easily broken down for repurposing or reuse. Several example layouts and workstations are presented.

  9. Predicting mutually exclusive spliced exons based on exon length, splice site and reading frame conservation, and exon sequence homology

    Directory of Open Access Journals (Sweden)

    Hammesfahr Björn

    2011-06-01

    Full Text Available Abstract Background Alternative splicing of pre-mature RNA is an important process eukaryotes utilize to increase their repertoire of different protein products. Several types of different alternative splice forms exist including exon skipping, differential splicing of exons at their 3'- or 5'-end, intron retention, and mutually exclusive splicing. The latter term is used for clusters of internal exons that are spliced in a mutually exclusive manner. Results We have implemented an extension to the WebScipio software to search for mutually exclusive exons. Here, the search is based on the precondition that mutually exclusive exons encode regions of the same structural part of the protein product. This precondition provides restrictions to the search for candidate exons concerning their length, splice site conservation and reading frame preservation, and overall homology. Mutually exclusive exons that are not homologous and not of about the same length will not be found. Using the new algorithm, mutually exclusive exons in several example genes, a dynein heavy chain, a muscle myosin heavy chain, and Dscam were correctly identified. In addition, the algorithm was applied to the whole Drosophila melanogaster X chromosome and the results were compared to the Flybase annotation and an ab initio prediction. Clusters of mutually exclusive exons might be subsequent to each other and might encode dozens of exons. Conclusions This is the first implementation of an automatic search for mutually exclusive exons in eukaryotes. Exons are predicted and reconstructed in the same run providing the complete gene structure for the protein query of interest. WebScipio offers high quality gene structure figures with the clusters of mutually exclusive exons colour-coded, and several analysis tools for further manual inspection. The genome scale analysis of all genes of the Drosophila melanogaster X chromosome showed that WebScipio is able to find all but two of the 28

  10. Research on a Frame-Based Model of Reading Comprehension. Final Report.

    Science.gov (United States)

    Goldstein, Ira

    This report summarizes computational investigations of language comprehension based on Marvin Minsky's theory of frames, a recent advance in artifical intelligence theories about the representation of knowledge. The investigations discussed explored frame theory as a basis for text comprehension by implementing models of the theory and developing…

  11. Online Prelectures: An Alternative to Textbook Reading Assignments

    Science.gov (United States)

    Sadaghiani, Homeyra R.

    2012-05-01

    To engage students in a more meaningful discussion of course material and prompt their higher thinking skills, most instructors expect students to read the course textbook for initial exposure to the course content before class. However, as many instructors are aware, most students do not read their textbook throughout the quarter.1,2 At California State Polytechnic University, Pomona (Cal Poly Pomona) we have adopted web-based multimedia learning modules (MLMs) as prelecture assignments to help students to prepare for the class activities. The MLMs place lecture contents into the hands and control of the learners; similar to "flipped"3 or "inverted"4 classroom approaches, this method allows students to receive key course content outside of class and apply and analyze the content actively during class. In addition to initial exposure to basic principle, the MLMs provide additional worked examples that cannot be thoroughly covered in class.

  12. 框架与框架化:再读Goffman之《框架分析》%Frame and Framing: Re-reading Frame Analysis

    Institute of Scientific and Technical Information of China (English)

    赵靓

    2015-01-01

    美国社会学家Erving Goffman关于框架(frame)、立场(footing)和结盟(alignment)的理论从象征互动的角度阐释人在社会中如何扮演自己的角色,社会角色是如何被分配、定义,以及人们在交际过程中怎样定位自我和他者等问题.目前国内有关框架理论的研究存在两个问题:第一,研究著作几乎都来自于传播学的课题;第二,对《框架分析》文本本身的研究稀少.该文对该著作的内容进行简单的述评,对其中一些术语进行梳理,并从社会语言学的角度探讨框架理论和话语分析的结合和运用.

  13. Facilitating Reading Acquisition in Multilingual Environments in India (FRAME-India). Final Report

    Science.gov (United States)

    Nakamura, Pooja; de Hoop, Thomas

    2014-01-01

    Most of the world is multilingual--multilingual at the national level (policies), at the community and family level (practices), and at the individual level (cognitive)--and each of these has implications for teaching and learning. Yet, at present, most reading decisions are not based on empirical research of how children learn to read in…

  14. An upstream open reading frame controls translation of var2csa, a gene implicated in placental malaria

    DEFF Research Database (Denmark)

    Amulic, Borko; Salanti, Ali; Lavstsen, Thomas

    2009-01-01

    to only be expressed in the presence of a placenta, suggesting that its expression is actively repressed in men, children or non-pregnant women; however, the mechanism of repression is not understood. Using cultured parasite lines and reporter gene constructs, we show that the gene encoding VAR2CSA...... contains a small upstream open reading frame that acts to repress translation of the resulting mRNA, revealing a novel form of gene regulation in malaria parasites. The mechanism underlying this translational repression is reversible, allowing high levels of protein translation upon selection, thus...... potentially enabling parasites to upregulate expression of this variant antigen in the presence of the appropriate host tissue....

  15. Identification and characterization of Kaposi's sarcoma-associated herpesvirus open reading frame 11 promotor activation

    Energy Technology Data Exchange (ETDEWEB)

    Chen, Lei [Los Alamos National Laboratory

    2008-01-01

    Open reading frame 11 (ORF11) of Kaposi's sarcoma-associated herpesvirus belongs to a herpesviral homologous protein family shared by some members of the gamma- herpesvirus subfamily. Little is known about this ORF11 homologous protein family. We have characterized an unknown open reading frame, ORF11, located adjacent and in the opposite orientation to a well-characterized viral IL-6 gene. Northern blot analysis reveals that ORF11 is expressed during the KSHV lytic cycle with delayed-early transcription kinetics. We have determined the 5{prime} and 3{prime} untranslated region of the unspliced ORF11 transcript and identified both the transcription start site and the transcription termination site. Core promoter region, representing ORF11 promoter activity, was mapped to a 159nt fragment 5{prime} most proximal to the transcription start site. A functional TATA box was identified in the core promoter region. Interestingly, we found that ORF11 transcriptional activation is not responsive to Rta, the KSHV lytic switch protein. We also discovered that part of the ORF11 promoter region, the 209nt fragment upstream of the transcription start site, was repressed by phorbol esters. Our data help to understand transcription regulation of ORF11 and to elucidate roles of ORF11 in KSHV pathogenesis and life cycle.

  16. Effects of Alternative Framing on the Publics Perceived Importance of Environmental Conservation

    Directory of Open Access Journals (Sweden)

    Amanda E Sorensen

    2015-05-01

    Full Text Available Effective communication of science to the general public is important for numerous reasons, including support for policy, funding, informed public decision making, among others. Prior research has found that scientists participating in public policy and public communication must frame their communication efforts in order to connect with audiences. A frame is the mechanism that individuals use to understand and interpret the world around them. Framing can encourage specific interpretations and reference points for a particular issue or event; especially when meaning is negotiated between the media and public audiences. In this study, we looked at the effect of framing within an environmental conservation context. To do this we had survey respondents rank common issues, among them being environmental conservation, from most important to least important for the government to address. We framed environmental conservation using three synonymous terms (environmental security, ecosystem services, and environmental quality to assess whether there was an effect on rankings dependent on how we framed environmental conservation. We also investigated the effect of individuals’ personality characteristics (identity frame on those environmental conservation rankings. We found that individuals who self-identified as environmentalist were positively associated with ranking highly (most important environmental conservation when it was framed as either environmental quality or ecosystem services, but not when it was framed as environmental security. Conversely, those individuals who did not rank themselves highly as self-identified environmentalists were positively associated with environmental conservation when it was framed as environmental security. This research suggests that framing audience specific messages can engender audience support in hot-button issues such as environmental conservation and climate change.

  17. A simple explanation for a case of incompatibility with the reading frame theory in Duchenne muscular dystrophy: failure to detect an aberrant restriction fragment in Southern blot analysis.

    Science.gov (United States)

    Patria, S Y; Takeshima, Y; Suminaga, R; Nakamura, H; Iwasaki, R; Minagawa, T; Matsuo, M

    1999-09-01

    According to the translational reading frame theory, Duchenne muscular dystrophy (DMD) patients harbor out-of-frame deletion mutations in the dystrophin gene. We identified a Japanese DMD case who appeared to have an in-frame deletion of exons 46-54 that was disclosed by Southern blot analysis using a dystrophin cDNA as a probe. Analysis of dystrophin mRNA in skeletal muscle revealed the presence of an out-of-frame deletion of exons 46-53. In agreement with this result, the region encompassing exon 54 could be amplified from genomic DNA by polymerase chain reaction (PCR). Furthermore, re-analysis by Southern blot using an exon specific probe disclosed that a HindIII fragment containing exon 54 was present at aberrant size, leading to the incorrect conclusion that exon 54 had been deleted. Thus, this particular DMD case does not constitute an exception to the reading frame theory.

  18. Repeat associated non-ATG translation initiation: one DNA, two transcripts, seven reading frames, potentially nine toxic entities!

    Directory of Open Access Journals (Sweden)

    Christopher E Pearson

    2011-03-01

    Full Text Available Diseases associated with unstable repetitive elements in the DNA, RNA, and amino acids have consistently revealed scientific surprises. Most diseases are caused by expansions of trinucleotide repeats, which ultimately lead to diseases like Huntington's disease, myotonic dystrophy, fragile X syndrome, and a series of spinocerebellar ataxias. These repeat mutations are dynamic, changing through generations and within an individual, and the repeats can be bi-directionally transcribed. Unsuspected modes of pathogenesis involve aberrant loss of protein expression; aberrant over-expression of non-mutant proteins; toxic-gain-of-protein function through expanded polyglutamine tracts that are encoded by expanded CAG tracts; and RNA-toxic-gain-of-function caused by transcripts harboring expanded CUG, CAG, or CGG tracts. A recent advance reveals that RNA transcripts with expanded CAG repeats can be translated in the complete absence of a starting ATG, and this Repeat Associated Non-ATG translation (RAN-translation occurs across expanded CAG repeats in all reading frames (CAG, AGC, and GCA to produce homopolymeric proteins of long polyglutamine, polyserine, and polyalanine tracts. Expanded CTG tracts expressing CUG transcripts also show RAN-translation occurring in all three frames (CUG, UGC, and GCU, to produce polyleucine, polycysteine, and polyalanine. These RAN-translation products can be toxic. Thus, one unstable (CAG•(CTG DNA can produce two expanded repeat transcripts and homopolymeric proteins with reading frames (the AUG-directed polyGln and six RAN-translation proteins, yielding a total of potentially nine toxic entities. The occurrence of RAN-translation in patient tissues expands our horizons of modes of disease pathogenesis. Moreover, since RAN-translation counters the canonical requirements of translation initiation, many new questions are now posed that must be addressed. This review covers RAN-translation and some of the pertinent

  19. Alternative Perspectives of Reading/Writing Connections. Occasional Paper No. 130.

    Science.gov (United States)

    McCarthey, Sarah J.; Raphael, Taffy E.

    This paper describes three alternative perspectives--information processing, social constructivism, and Piagetian/naturalist--of reading/writing connections and suggests instructional implications influenced by the three perspectives. The paper explores each theory in terms of basic assumptions, related research, and strengths and limitations and…

  20. The phenotype of many independently isolated +1 frameshift suppressor mutants supports a pivotal role of the P-site in reading frame maintenance.

    Science.gov (United States)

    Jäger, Gunilla; Nilsson, Kristina; Björk, Glenn R

    2013-01-01

    The main features of translation are similar in all organisms on this planet and one important feature of it is the way the ribosome maintain the reading frame. We have earlier characterized several bacterial mutants defective in tRNA maturation and found that some of them correct a +1 frameshift mutation; i.e. such mutants possess an error in reading frame maintenance. Based on the analysis of the frameshifting phenotype of such mutants we proposed a pivotal role of the ribosomal grip of the peptidyl-tRNA to maintain the correct reading frame. To test the model in an unbiased way we first isolated many (467) independent mutants able to correct a +1 frameshift mutation and thereafter tested whether or not their frameshifting phenotypes were consistent with the model. These 467+1 frameshift suppressor mutants had alterations in 16 different loci of which 15 induced a defective tRNA by hypo- or hypermodifications or altering its primary sequence. All these alterations of tRNAs induce a frameshift error in the P-site to correct a +1 frameshift mutation consistent with the proposed model. Modifications next to and 3' of the anticodon (position 37), like 1-methylguanosine, are important for proper reading frame maintenance due to their interactions with components of the ribosomal P-site. Interestingly, two mutants had a defect in a locus (rpsI), which encodes ribosomal protein S9. The C-terminal of this protein contacts position 32-34 of the peptidyl-tRNA and is thus part of the P-site environment. The two rpsI mutants had a C-terminal truncated ribosomal protein S9 that destroys its interaction with the peptidyl-tRNA resulting in +1 shift in the reading frame. The isolation and characterization of the S9 mutants gave strong support of our model that the ribosomal grip of the peptidyl-tRNA is pivotal for the reading frame maintenance.

  1. Sub-electron read noise and millisecond full-frame readout with the near infrared eAPD array SAPHIRA

    Science.gov (United States)

    Finger, Gert; Baker, Ian; Alvarez, Domingo; Dupuy, Christophe; Ives, Derek; Meyer, Manfred; Mehrgan, Leander; Stegmeier, Jörg; Weller, Harald J.

    2016-07-01

    In 2007 ESO started a program at SELEX (now LEONARDO) to develop noiseless near infrared HgCdTe electron avalanche photodiode arrays (eAPD)[1][2][3]. This eAPD technology is only way to overcome the limiting CMOS noise barrier of near infrared sensors used for wavefront sensing and fringe tracking. After several development cycles of solid state engineering techniques which can be easily applied to the chosen growth technology of metal organic vapour phase epitaxy (MOVPE), the eAPD arrays have matured and resulted in the SAPHIRA arrays. They have a format of 320x256 pixels with a pitch of 24 μm. They now offer an unmatched combination of sub-electron read noise at millisecond frame readout rates. The first generation of SAPHIRA arrays were only sensitive in H and K-band. With the removal of a wide bandgap buffer layer the arrays are now sensitive from λ=0.8 μm to 2.5 μm with high quantum efficiency over the entire wavelength range. The high temperature anneal applied during the growth process produces material with superb cosmetic quality at an APD gain of over 600. The design of the SAPHIRA ROIC has also been revised and the new ME1000 ROIC has an optimized analogue chain and more flexible readout modes. The clock for the vertical shift register is now under external control. The advantage of this is that correlated-double-sampling and uncorrelated readout in the rolling shutter mode now have a duty cycle of 100% at the maximum frame rate. Furthermore, to reduce the readout noise rows can be read several times before and after row reset. Since the APD gain is sufficiently high that one photon produces many more electrons than the square root of kTC which is the charge uncertainty after reset, signals of one photon per exposure can be easily detected without the need for double correlated sampling. First results obtained with the fringe tracker in GRAVITY and the four SAPHIRA wavefront sensors installed in the CIAO adaptive optics systems of the four 8 meter

  2. Rational genomics I: antisense open reading frames and codon bias in short-chain oxido reductase enzymes and the evolution of the genetic code.

    Science.gov (United States)

    Duax, William L; Huether, Robert; Pletnev, Vladimir Z; Langs, David; Addlagatta, Anthony; Connare, Sonjay; Habegger, Lukas; Gill, Jay

    2005-12-01

    The short-chain oxidoreductase (SCOR) family of enzymes includes over 6000 members, extending from bacteria and archaea to humans. Nucleic acid sequence analysis reveals that significant numbers of these genes are remarkably free of stopcodons in reading frames other than the coding frame, including those on the antisense strand. The genes from this subset also use almost entirely the GC-rich half of the 64 codons. Analysis of a million hypothetical genes having random nucleotide composition shows that the percentage of SCOR genes having multiple open reading frames exceeds random by a factor of as much as 1 x 10(6). Nevertheless, screening the content of the SWISS-PROT TrEMBL database reveals that 15% of all genes contain multiple open reading frames. The SCOR genes having multiple open reading frames and a GC-rich coding bias exhibit a similar GC bias in the nucleotide triple composition of their DNA. This bias is not correlated with the GC content of the species in which the SCOR genes are found. One possible explanation for the conservation of multiple open reading frames and extreme bias in nucleic acid composition in the family of Rossman folds is that the primordial member of this family was encoded early using only very stable GC-rich DNA and that evolution proceeded with extremely limited introduction of any codons having two or more adenine or thymine nucleotides. These and other data suggest that the SCOR family of enzymes may even have diverged from a common ancestor before most of the AT-rich half of the genetic code was fully defined.

  3. Role of the Hepatitis C Virus Core+1 Open Reading Frame and Core cis-Acting RNA Elements in Viral RNA Translation and Replication▿ †

    Science.gov (United States)

    Vassilaki, Niki; Friebe, Peter; Meuleman, Philipe; Kallis, Stephanie; Kaul, Artur; Paranhos-Baccalà, Glaucia; Leroux-Roels, Geert; Mavromara, Penelope; Bartenschlager, Ralf

    2008-01-01

    Four conserved RNA stem-loop structures designated SL47, SL87, SL248, and SL443 have been predicted in the hepatitis C virus (HCV) core encoding region. Moreover, alternative translation products have been detected from a reading frame overlapping the core gene (core+1/ARFP/F). To study the importance of the core+1 frame and core-RNA structures for HCV replication in cell culture and in vivo, a panel of core gene silent mutations predicted to abolish core+1 translation and affecting core-RNA stem-loops were introduced into infectious-HCV genomes of the isolate JFH1. A mutation disrupting translation of all known forms of core+1 and affecting SL248 did not alter virus production in Huh7 cells and in mice xenografted with human liver tissue. However, a combination of mutations affecting core+1 at multiple codons and at the same time, SL47, SL87, and SL248, delayed RNA replication kinetics and substantially reduced virus titers. The in vivo infectivity of this mutant was impaired, and in virus genomes recovered from inoculated mice, SL87 was restored by reversion and pseudoreversion. Mutations disrupting the integrity of this stem-loop, as well as that of SL47, were detrimental for virus viability, whereas mutations disrupting SL248 and SL443 had no effect. This phenotype was not due to impaired RNA stability but to reduced RNA translation. Thus, SL47 and SL87 are important RNA elements contributing to HCV genome translation and robust replication in cell culture and in vivo. PMID:18799568

  4. Human Cytomegalovirus UL138 Open Reading Frame Is Highly Conserved in Clinical Strains

    Institute of Scientific and Technical Information of China (English)

    Ying Qi; Rong He; Yan-ping Ma; Zheng-rong Sun; Yao-hua Ji; Qiang Ruan

    2009-01-01

    To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading flame (ORF) in clinical strains.Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains.Results UL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities of UL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleotide mutations were substitutions. The spatial structure and post-translational modification sites of UL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV UL138 sequence variations were not definitely related with different clinical symptoms.Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.

  5. Who needs RDD? Combining directory listings with cell phone exchanges for an alternative telephone sampling frame

    OpenAIRE

    Guterbock, Thomas; Diop, Abdoulaye; Ellis, James; Trung Le, Kien

    2011-01-01

    Abstract The traditional Random Digit Dialing method (list-assisted RDD using a frame of landline phone numbers) is clearly under threat. The difficulty and costs of completing telephone surveys have increased due to rising rates of refusal and non-contact. The completeness of coverage of list-assisted RDD samples has diminished due to the proliferation of cell-phone only households. The ability of list-assisted RDD to capture young, mobile, unmarried, and minority households is thus dimin...

  6. Human herpesvirus 8 open reading frame 26 and open reading frame 65 sequences from multiple myeloma patients: a shared pattern not found in Kaposi's sarcoma or primary effusion lymphoma.

    Science.gov (United States)

    Ma, H J; Sjak-Shie, N N; Vescio, R A; Kaminsky, M; Mikail, A; Pold, M; Parker, K; Beksac, M; Belson, D; Moss, T J; Wu, C H; Zhou, J; Zhang, L; Chen, G; Said, J W; Berenson, J R

    2000-11-01

    Human herpesvirus 8 (HHV-8), also known as Kaposi's sarcoma-associated herpesvirus, has been implicated in the pathogenesis of Kaposi's sarcoma (KS), primary effusion lymphoma (PEL), multicentric Castleman's disease, and recently multiple myeloma (MM). DNA sequence analyses of HHV-8 suggest that multiple HHV-8 strains exist. We extracted DNA from 24 patients with MM and 3 patients with monoclonal gammopathy of undetermined significance and compared HHV-8 open reading frames (ORFs) 26 and 65 sequences with those derived from patients with KS, PEL, and two HHV-8-positive PEL cell lines KS-1 and BC-1. ORF26 sequence data suggest that MM patients are consistently carriers of HHV-8 strain subtype C3. All MM patients also consistently revealed either a single bp deletion or substitution at position 112197 in ORF65. This unique alteration is not present in patients with KS or PEL or in PEL cell lines. It occurs in the portion of ORF65 that is known to be responsible for a serological response to HHV-8.

  7. An alternative approach for teacher education framed by a collaborative partnership setting

    DEFF Research Database (Denmark)

    Pontoppidan, Birgitte Schou

    2010-01-01

    The study presents an alternative didactical approach to teacher education linking practice and theory through a collaborative partnership setting. Using a ―small scale teaching design in which students alternate between schools and college it was possible to show someevidence that, by following...... teacher education on its head and begins with a focus on practice so students alternate between school–based and college–based teaching in a cyclical fashion, and are encouraged to link theory with practice. This kind of college teaching demands a new teacher educational paradigm for which collaboration...... between schools and colleges is paramount. This study indicates that a collaborative partnership setting involving college and S&T- profile schools can provide a ―room for study, and that this kind of teaching design enhances the opportunities for students‘ to develop teacher knowledge (aspects...

  8. AN ALTERNATIVE APPROACH FOR TEACHER EDUCATION COURSES FRAMED BY A COLLABORATE PARTNERSHIP

    DEFF Research Database (Denmark)

    Pontoppidan, Birgitte

    2010-01-01

    The study presents an alternative didactical approach to teacher education linking practice and theory through a collaborative partnership setting. Using a “small scale” teaching design in which students alternate between schools and college it was possible to show some evidence that, by following...... teacher education on its head and begins with a focus on practice so students alternate between school–based and college–based teaching in a cyclical fashion, and are encouraged to link theory with practice. This kind of college teaching demands a new teacher educational paradigm for which collaboration...... between schools and colleges is paramount. This study indicates that a collaborative partnership setting involving college and S&T- profile schools can provide a “room for study”, and that this kind of teaching design enhances the opportunities for students’ to develop teacher knowledge (aspects...

  9. Duck hepatitis B virus expresses a regulatory HBx-like protein from a hidden open reading frame.

    Science.gov (United States)

    Chang, S F; Netter, H J; Hildt, E; Schuster, R; Schaefer, S; Hsu, Y C; Rang, A; Will, H

    2001-01-01

    Duck hepatitis B viruses (DHBV), unlike mammalian hepadnaviruses, are thought to lack X genes, which encode transcription-regulatory proteins believed to contribute to the development of hepatocellular carcinoma. A lack of association of chronic DHBV infection with hepatocellular carcinoma development supports this belief. Here, we demonstrate that DHBV genomes have a hidden open reading frame from which a transcription-regulatory protein, designated DHBx, is expressed both in vitro and in vivo. We show that DHBx enhances neither viral protein expression, intracellular DNA synthesis, nor virion production when assayed in the full-length genome context in LMH cells. However, similar to mammalian hepadnavirus X proteins, DHBx activates cellular and viral promoters via the Raf-mitogen-activated protein kinase signaling pathway and localizes primarily in the cytoplasm. The functional similarities as well as the weak sequence homologies of DHBx and the X proteins of mammalian hepadnaviruses strongly suggest a common ancestry of ortho- and avihepadnavirus X genes. In addition, our data disclose similar intracellular localization and transcription regulatory functions of the corresponding proteins, raise new questions as to their presumed role in hepatocarcinogenesis, and imply unique opportunities for deciphering of their still-enigmatic in vivo functions.

  10. Lncident: A Tool for Rapid Identification of Long Noncoding RNAs Utilizing Sequence Intrinsic Composition and Open Reading Frame Information

    Directory of Open Access Journals (Sweden)

    Siyu Han

    2016-01-01

    Full Text Available More and more studies have demonstrated that long noncoding RNAs (lncRNAs play critical roles in diversity of biological process and are also associated with various types of disease. How to rapidly identify lncRNAs and messenger RNA is the fundamental step to uncover the function of lncRNAs identification. Here, we present a novel method for rapid identification of lncRNAs utilizing sequence intrinsic composition features and open reading frame information based on support vector machine model, named as Lncident (LncRNAs identification. The 10-fold cross-validation and ROC curve are used to evaluate the performance of Lncident. The main advantage of Lncident is high speed without the loss of accuracy. Compared with the exiting popular tools, Lncident outperforms Coding-Potential Calculator, Coding-Potential Assessment Tool, Coding-Noncoding Index, and PLEK. Lncident is also much faster than Coding-Potential Calculator and Coding-Noncoding Index. Lncident presents an outstanding performance on microorganism, which offers a great application prospect to the analysis of microorganism. In addition, Lncident can be trained by users’ own collected data. Furthermore, R package and web server are simultaneously developed in order to maximize the convenience for the users. The R package “Lncident” can be easily installed on multiple operating system platforms, as long as R is supported.

  11. Non-coding RNA identification based on topology secondary structure and reading frame in organelle genome level.

    Science.gov (United States)

    Wu, Cheng-Yan; Li, Qian-Zhong; Feng, Zhen-Xing

    2016-01-01

    Non-coding RNA (ncRNA) genes make transcripts as same as the encoding genes, and ncRNAs directly function as RNAs rather than serve as blueprints for proteins. As the function of ncRNA is closely related to organelle genomes, it is desirable to explore ncRNA function by confirming its provenance. In this paper, the topology secondary structure, motif and the triplets under three reading frames are considered as parameters of ncRNAs. A method of SVM combining the increment of diversity (ID) algorithm is applied to construct the classifier. When the method is applied to the ncRNA dataset less than 80% sequence identity, the overall accuracies reach 95.57%, 96.40% in the five-fold cross-validation and the jackknife test, respectively. Further, for the independent testing dataset, the average prediction success rate of our method achieved 93.24%. The higher predictive success rates indicate that our method is very helpful for distinguishing ncRNAs from various organelle genomes.

  12. Cloning of open reading frames and promoters from the Saccharomyces cerevisiae genome: construction of genomic libraries of random small fragments.

    Science.gov (United States)

    Santangelo, G M; Tornow, J; Moldave, K

    1986-01-01

    We have developed a novel efficient method, carrier-facilitated insertion, to insert small (150-600 bp) DNA fragments into plasmid vectors. This method employs a carrier segment of vector DNA to circumvent the difficulties in ligating two fragments together to generate a recombinant circle efficiently. We have used carrier-facilitated insertion to construct three genomic libraries of random (DNase I-generated) fragments from the Saccharomyces cerevisiae genome. One of these was an expression library, and the other two were promoter-cloning libraries. 87-90% of the Escherichia coli colonies in each library contained recombinant plasmids, and less than 3% of the recombinants contained more than one insert. Detection of open reading frames among the inserts in the expression library was accomplished by testing for beta-galactosidase activity. This methodology, unencumbered by the intrinsic disproportionality of cDNA libraries, can be used to identify and clone DNA that codes for a specific antigenic determinant. When used in combination with a method to detect and isolate random constitutive, repressible and inducible yeast promoters, these libraries should permit a comprehensive analysis of the yeast genome and its expression.

  13. Translocations used to generate chromosome segment duplications in Neurospora can disrupt genes and create novel open reading frames

    Indian Academy of Sciences (India)

    Parmit K Singh; Srividhya V Iyer; T Naga Sowjanya; B Kranthi Raj; Durgadas P Kasbekar

    2010-12-01

    In Neurospora crassa, crosses between normal sequence strains and strains bearing some translocations can yield progeny bearing a duplication (Dp) of the translocated chromosome segment. Here, 30 breakpoint junction sequences of 12 Dp-generating translocations were determined. The breakpoints disrupted 13 genes (including predicted genes), and created 10 novel open reading frames. Insertion of sequences from LG III into LG I as translocation T(UK818) disrupts the eat-3 gene, which is the ortholog of the Podospora anserine gene ami1. Since ami1-homozygous Podospora crosses were reported to increase the frequency of repeat-induced point mutation (RIP), we performed crosses homozygous for a deficiency in eat-3 to test for a corresponding increase in RIP frequency. However, our results suggested that, unlike in Podospora, the eat-3 gene might be essential for ascus development in Neurospora. Duplication–heterozygous crosses are generally barren in Neurospora; however, by using molecular probes developed in this study, we could identify Dp segregants from two different translocation–heterozygous crosses, and using these we found that the barren phenotype of at least some duplication–heterozygous crosses was incompletely penetrant.

  14. Varicella-zoster virus (VZV) mediates a delayed host shutoff independent of open reading frame (ORF) 17 expression.

    Science.gov (United States)

    Waterboer, Tim; Rahaus, Markus; Wolff, Manfred H

    2002-01-01

    Varicella-zoster virus (VZV) open reading frame 17 (ORF 17) is the gene corresponding to Herpes simplex-virus (HSV) UL41. The UL41 gene encodes the virion host shutoff factor (vhs), a RNase that has been the object of detailed studies. In contrast to HSV, knowledge about VZV mediated shutoff effects and the role of ORF 17 is poor. We investigated the ORF 17 expression in infected cells and analyzed shutoff effects. ORF 17 expression could not be proven in infected human fibroblast cell lines and melanoma (MeWo) cells. Only after induction by Phorbol 12-myristate 13-acetate an ORF 17 expression became detectable in MeWo cells. Nevertheless, using stable expressed GAPDH mRNA as a marker for mRNA degradation, a VZV mediated shutoff, independent of ORF 17 expression, became measurable. Transfection experiments demonstrated that transient ORF 17 expression did not decrease the cellular GAPDH mRNA level. We examined whether the VZV shutoff factor is a tegument protein causing an early shutoff or whether it needs to be expressed (delayed shutoff). The GAPDH mRNA level in Actinomycin D pretreated and infected MeWo cells did not decrease even faster than the theoretical decay rate based on a half-life of 24 h. These findings lead to the conclusion that the VZV shutoff factor is not a mature protein localized in the virion and that VZV causes a delayed virion host shutoff effect.

  15. Cytoplasmic male sterility-associated chimeric open reading frames identified by mitochondrial genome sequencing of four Cajanus genotypes.

    Science.gov (United States)

    Tuteja, Reetu; Saxena, Rachit K; Davila, Jaime; Shah, Trushar; Chen, Wenbin; Xiao, Yong-Li; Fan, Guangyi; Saxena, K B; Alverson, Andrew J; Spillane, Charles; Town, Christopher; Varshney, Rajeev K

    2013-10-01

    The hybrid pigeonpea (Cajanus cajan) breeding technology based on cytoplasmic male sterility (CMS) is currently unique among legumes and displays major potential for yield increase. CMS is defined as a condition in which a plant is unable to produce functional pollen grains. The novel chimeric open reading frames (ORFs) produced as a results of mitochondrial genome rearrangements are considered to be the main cause of CMS. To identify these CMS-related ORFs in pigeonpea, we sequenced the mitochondrial genomes of three C. cajan lines (the male-sterile line ICPA 2039, the maintainer line ICPB 2039, and the hybrid line ICPH 2433) and of the wild relative (Cajanus cajanifolius ICPW 29). A single, circular-mapping molecule of length 545.7 kb was assembled and annotated for the ICPA 2039 line. Sequence annotation predicted 51 genes, including 34 protein-coding and 17 RNA genes. Comparison of the mitochondrial genomes from different Cajanus genotypes identified 31 ORFs, which differ between lines within which CMS is present or absent. Among these chimeric ORFs, 13 were identified by comparison of the related male-sterile and maintainer lines. These ORFs display features that are known to trigger CMS in other plant species and to represent the most promising candidates for CMS-related mitochondrial rearrangements in pigeonpea.

  16. An upstream open reading frame controls translation of var2csa, a gene implicated in placental malaria.

    Directory of Open Access Journals (Sweden)

    Borko Amulic

    2009-01-01

    Full Text Available Malaria, caused by the parasite Plasmodium falciparum, is responsible for substantial morbidity, mortality and economic losses in tropical regions of the world. Pregnant women are exceptionally vulnerable to severe consequences of the infection, due to the specific adhesion of parasite-infected erythrocytes in the placenta. This adhesion is mediated by a unique variant of PfEMP1, a parasite encoded, hyper-variable antigen placed on the surface of infected cells. This variant, called VAR2CSA, binds to chondroitin sulfate A on syncytiotrophoblasts in the intervillous space of placentas. VAR2CSA appears to only be expressed in the presence of a placenta, suggesting that its expression is actively repressed in men, children or non-pregnant women; however, the mechanism of repression is not understood. Using cultured parasite lines and reporter gene constructs, we show that the gene encoding VAR2CSA contains a small upstream open reading frame that acts to repress translation of the resulting mRNA, revealing a novel form of gene regulation in malaria parasites. The mechanism underlying this translational repression is reversible, allowing high levels of protein translation upon selection, thus potentially enabling parasites to upregulate expression of this variant antigen in the presence of the appropriate host tissue.

  17. Mapping of the nuclear localization signals in open reading frame 2 protein from porcine circovirus type 1

    Institute of Scientific and Technical Information of China (English)

    Jiangbing Shuai; Wei Wei; Lingli Jiang; Xiaoliang Li; Ning Chen; Weihuan Fang

    2008-01-01

    Porcine circovirus type 1 (PCV1) contains two major open reading frames encoding the replication-associated proteins and the major structural capsid (Cap) protein.PCV1 Cap has an N-terminus carrying several potential monopartite or bipartite nuclear localization signals (NLS).The contribution of these partially overlapping motifs to nuclear importing was identified by expression of mutated PCV1 Cap versions fused to enhanced green fluorescent protein (EGFP).The Cterminus truncated PCV1 Cap-EGFP was localized in nuclei of PK-15 cells similar to the wild-type PCV1 Cap-EGFP,whereas truncation of the N-terminus rendered the fusion protein distributed into cytoplasm,indicating that the nuclear import of PCV1 Cap was efficiently mediated by its N-terminal region.Substitutions of basic residues in stretches 9RRRR12 or the right part of 25RRPYLAHPAFRNRYRWRRK43 resulted in a diffused distribution of the fusion protein in both nuclei and cytoplasm,indicating that the two NLSs were responsible for restricted nuclear targeting of PCV1 Cap.

  18. Framing the frame

    Directory of Open Access Journals (Sweden)

    Todd McElroy

    2007-08-01

    Full Text Available We examined how the goal of a decision task influences the perceived positive, negative valence of the alternatives and thereby the likelihood and direction of framing effects. In Study 1 we manipulated the goal to increase, decrease or maintain the commodity in question and found that when the goal of the task was to increase the commodity, a framing effect consistent with those typically observed in the literature was found. When the goal was to decrease, a framing effect opposite to the typical findings was observed whereas when the goal was to maintain, no framing effect was found. When we examined the decisions of the entire population, we did not observe a framing effect. In Study 2, we provided participants with a similar decision task except in this situation the goal was ambiguous, allowing us to observe participants' self-imposed goals and how they influenced choice preferences. The findings from Study 2 demonstrated individual variability in imposed goal and provided a conceptual replication of Study 1. %need keywords

  19. The phenotype of many independently isolated +1 frameshift suppressor mutants supports a pivotal role of the P-site in reading frame maintenance.

    Directory of Open Access Journals (Sweden)

    Gunilla Jäger

    Full Text Available The main features of translation are similar in all organisms on this planet and one important feature of it is the way the ribosome maintain the reading frame. We have earlier characterized several bacterial mutants defective in tRNA maturation and found that some of them correct a +1 frameshift mutation; i.e. such mutants possess an error in reading frame maintenance. Based on the analysis of the frameshifting phenotype of such mutants we proposed a pivotal role of the ribosomal grip of the peptidyl-tRNA to maintain the correct reading frame. To test the model in an unbiased way we first isolated many (467 independent mutants able to correct a +1 frameshift mutation and thereafter tested whether or not their frameshifting phenotypes were consistent with the model. These 467+1 frameshift suppressor mutants had alterations in 16 different loci of which 15 induced a defective tRNA by hypo- or hypermodifications or altering its primary sequence. All these alterations of tRNAs induce a frameshift error in the P-site to correct a +1 frameshift mutation consistent with the proposed model. Modifications next to and 3' of the anticodon (position 37, like 1-methylguanosine, are important for proper reading frame maintenance due to their interactions with components of the ribosomal P-site. Interestingly, two mutants had a defect in a locus (rpsI, which encodes ribosomal protein S9. The C-terminal of this protein contacts position 32-34 of the peptidyl-tRNA and is thus part of the P-site environment. The two rpsI mutants had a C-terminal truncated ribosomal protein S9 that destroys its interaction with the peptidyl-tRNA resulting in +1 shift in the reading frame. The isolation and characterization of the S9 mutants gave strong support of our model that the ribosomal grip of the peptidyl-tRNA is pivotal for the reading frame maintenance.

  20. Differences in lower limb transverse plane joint moments during gait when expressed in two alternative reference frames.

    Science.gov (United States)

    Schache, Anthony G; Baker, Richard; Vaughan, Christopher L

    2007-01-01

    When comparing previous studies that have measured the three-dimensional moments acting about the lower limb joints (either external moments or opposing internal joint moments) during able-bodied adult gait, significant variation is apparent in the profiles of the reported transverse plane moments. This variation cannot be explained on the basis of adopted convention (i.e. external versus internal joint moment) or inherent variability in gait strategies. The aim of the current study was to determine whether in fact the frame in which moments are expressed has a dominant effect upon transverse plane moments and thus provides a valid explanation for the observed inconsistency in the literature. Kinematic and ground reaction force data were acquired from nine able-bodied adult subjects walking at a self-selected speed. Three-dimensional hip, knee and ankle joint moments during gait were calculated using a standard inverse dynamics approach. In addition to calculating internal joint moments, the components of the external moment occurring in the transverse plane at each of the lower limb joints were calculated to determine their independent effects. All moments were expressed in both the laboratory frame (LF) as well as the anatomical frame (AF) of the distal segment. With the exception of the ankle rotation moment in the foot AF, lower limb transverse plane joint moments during gait were found to display characteristic profiles that were consistent across subjects. Furthermore, lower limb transverse plane joint moments during gait differed when expressed in the distal segment AF compared to the LF. At the hip, the two alternative reference frames produced near reciprocal joint moment profiles. The components of the external moment revealed that the external ground reaction force moment was primarily responsible for this result. Lower limb transverse plane joint moments during gait were therefore found to be highly sensitive to a change in reference frame. These

  1. Conserved-peptide upstream open reading frames (CPuORFs are associated with regulatory genes in angiosperms

    Directory of Open Access Journals (Sweden)

    Richard A Jorgensen

    2012-08-01

    Full Text Available Upstream open reading frames (uORFs are common in eukaryotic transcripts, but those that encode conserved peptides (CPuORFs occur in less than 1% of transcripts. The peptides encoded by three plant CPuORF families are known to control translation of the downstream ORF in response to a small signal molecule (sucrose, polyamines and phosphocholine. In flowering plants, transcription factors are statistically over-represented among genes that possess CPuORFs, and in general it appeared that many CPuORF genes also had other regulatory functions, though the significance of this suggestion was uncertain (Hayden and Jorgensen, 2007. Five years later the literature provides much more information on the functions of many CPuORF genes. Here we reassess the functions of 27 known CPuORF gene families and find that 22 of these families play a variety of different regulatory roles, from transcriptional control to protein turnover, and from small signal molecules to signal transduction kinases. Clearly then, there is indeed a strong association of CPuORFs with regulatory genes. In addition, 16 of these families play key roles in a variety of different biological processes. Most strikingly, the core sucrose response network includes three different CPuORFs, creating the potential for sophisticated balancing of the network in response to three different molecular inputs. We propose that the function of most CPuORFs is to modulate translation of a downstream major ORF (mORF in response to a signal molecule recognized by the conserved peptide and that because the mORFs of CPuORF genes generally encode regulatory proteins, many of them centrally important in the biology of plants, CPuORFs play key roles in balancing such regulatory networks.

  2. Disruption of six open reading frames on chromosome X of Saccharomyces cerevisiae reveals a cluster of four essential genes.

    Science.gov (United States)

    Esser, K; Scholle, B; Michaelis, G

    1999-07-01

    In this study we report the construction and basic phenotypic analysis of six Saccharomyces cerevisiae deletion mutants. The open reading frames (ORFs) YJL008C (gene symbol CCT8), YJL010C, YJL011C, YJL012C, YJL017W, and YJL020C from chromosome X have been disrupted by integration of deletion cassettes, comprising the bacterial KanMX4 marker gene and terminal long (LFH) or short (SFH) flanking sequences that are homologous to the 5' and 3' untranslated regions of the respective ORFs. For correct disruption of ORF YJL008C, it was necessary to construct a deletion cassette flanked by 300-350 bp long target guide sequences by LFH-PCR. Transformations using ORF YJL008C gene disruption cassettes synthesized by standard SFH-PCR exclusively resulted in false-positive or multiple integration events, probably because seven additional genes homologous to CCT8 exist in the yeast genome. The other five ORFs have been disrupted using cassettes generated by SFH-PCR, comprising terminal homologous regions of approximately 50 bp to each target site. Correct genomic integration of the reporter modules was verified by analytical PCR and Southern hybridization. Deletion of YJL008C, YJL010C, YJL011C, and YJL012C was found to be lethal, as shown by sporulation and tetrad analysis. This result is in contrast to the finding that only 16-20% of the genes in S. cerevisiae are estimated to be essential. The four essential genes described in this work are clustered, while the two other non-essential ORFs are separated by further ORFs. Although the two viable deletion mutants were tested against 60 different inhibitors, heavy metal ions and salts, no phenotype could be detected that co-segregated with the deletion during meiosis.

  3. The effects of direct instruction on the single-word reading skills of children who require augmentative and alternative communication.

    Science.gov (United States)

    Fallon, Karen A; Light, Janice; McNaughton, David; Drager, Kathryn; Hammer, Carol

    2004-12-01

    Current literature suggests a lack of empirically validated strategies for teaching reading skills to children who use augmentative and alternative communication (AAC). The current study implemented a single-subject, multiple-probe-across-subjects design to investigate the effects of direct instruction in single-word reading on the performance of students who use AAC. The instructional program targeted the reading skills of 5 participants who had severe speech impairments and ranged in age from 9 to 14 years old. All 5 participants reached criterion for matching targeted written words to corresponding pictures. Three of the 5 participants demonstrated generalization of reading skills to novel-word reading, and 4 of the 5 generalized reading skills to book contexts. Implications and directions for future research are discussed.

  4. Effects of a virtual platform in reading comprehension and vocabulary: An alternative to improve reading abilities in Elementary school

    Directory of Open Access Journals (Sweden)

    Cecilia Thorne

    2013-06-01

    Full Text Available Given, on the one hand, the poor results obtained by Peruvian children in the national and international reading assessments. And on the other hand, the increased investment intechnology for schools in the country, this study aimed to develop and test an online tool to improve reading comprehension. In order to do this, the reading comprehension strategies and vocabulary activities from the research-based digital environment ICON were adapted to design the platform LEO. A total of 88 fifth graders from urban middle-to-low-income private schools from Lima participated in this quasi-experimental study, which involved acontrol group and a treatment group that participated in a 12-week teacher-mediated digital intervention. All participants were administered reading and vocabulary assessments pre and post intervention. Results revealed that students who participated in the intervention achieved higher comprehension scores for narrative texts and higher vocabulary scores than those of the control group.

  5. Reading.

    Science.gov (United States)

    Gee, James Paul

    1992-01-01

    Explores what is meant by reading, noting that to read is to respond appropriately to a specific consensus centered on certain values and that the consensus is achieved among persons whose paths through life have come together with members of dominant discourses in society. (SLD)

  6. Determination of the sequence of the complete open reading frame and the 5 ' NTR of the Paderborn isolate of classical swine fever virus

    DEFF Research Database (Denmark)

    Oleksiewicz, Martin B.; Rasmussen, Thomas Bruun; Normann, Preben

    2003-01-01

    sequence of the 5' NTR (by 5' RACE) and the complete open reading frame of the Paderborn isolate (GenBank AY072924). Our sequence was identical to previously published partial 5'NTR and E2 sequences for the index Paderborn 1997 and Dutch 1997 (Venhorst) isolates, confirming the identity of the virus we....... Paderborn is the only recent European CSFV field isolate for which a complete sequence is available, and given Paderborns genetic and antigenic uniqueness, the Paderborn sequence may have practical use for diagnostic and vaccine antigen development....

  7. The complete sequence of a 9000 bp fragment of the right arm of Saccharomyces cerevisiae chromosome VII contains four previously unknown open reading frames.

    Science.gov (United States)

    Guerreiro, P; Silva, A M; Barreiros, T; Arroyo, J; García-Gonzalez, M; García-Saez, M I; Rodrigues-Pousada, C; Nombela, C

    1995-09-15

    We report the sequence of a 9000 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII. Analysis of the sequence revealed four complete previously unknown open reading frames, which were named G7587, G7589, G7591 and G7594 following standard rules for provisional nomenclature. Outstanding features of some of these proteins were the homology of the putative protein coded by G7589 with proteins involved in transcription regulation and the transmembrane domains predicted in the putative protein coded by G7591.

  8. Redes alternativas de comunicación, framing y la construcción del poder político (Alternative communication networks, framing and construction of political power

    Directory of Open Access Journals (Sweden)

    Mayra Martínez Avidad

    2011-12-01

    Full Text Available El objetivo del presente artículo es analizar el papel de las redes alternativas de comunicación en la construcción del poder político a través del proceso de framing o enmarcado. En los últimos años, la capacidad del público para producir (nuestros propios mensajes que potencialmente desafíen los encuadres fijados por la elite política se ha expandido enormemente con la llegada de Internet y las comunicaciones móviles. En este sentido, las redes virtuales son importantes porque pueden aumentar la capacidad de las masas para comunicarse entre sí transformando los marcos de referencia individuales en marcos interpretativos de carácter colectivo.The aim of this paper is to analyze the role of alternative communication networks in the construction of political power through the process of framing. In recent years, the ability of the public to produce (our own messages that potentially challenge those frames established by political elites has been importantly expanded by the advent of Internet and mobile communications. In this sense, virtual networks are important because they can influence the capacity of masses to communicate with each other transforming individual frames of reference into collective interpretative frames.

  9. Using Simulations to Investigate the Longitudinal Stability of Alternative Schemes for Classifying and Identifying Children with Reading Disabilities

    Science.gov (United States)

    Schatschneider, Christopher; Wagner, Richard K.; Hart, Sara A.; Tighe, Elizabeth L.

    2016-01-01

    The present study employed data simulation techniques to investigate the 1-year stability of alternative classification schemes for identifying children with reading disabilities. Classification schemes investigated include low performance, unexpected low performance, dual-discrepancy, and a rudimentary form of constellation model of reading…

  10. Alternative Text Types to Improve Reading Fluency for Competent to Struggling Readers

    Directory of Open Access Journals (Sweden)

    Timothy V. Rasinski

    2016-01-01

    Full Text Available This article offers instructional suggestions and strategies based on research and theoretical literature for developing reading fluency through the use of rhyming poetry and other texts beyond the narrative and informational texts that have been traditionally used for reading instruction. Readers’ lack of fluency in reading can be a monumental impediment to proficiency in good comprehension and overall reading competency. For all readers it is well established that as they progress in reading competence their reading ability grows (Stanovich, 1993/1994. This continued reading success begets continued reading growth; however, many struggling readers have difficulty in moving to a level of automaticity and fluency in their reading that enables them to engage in a successful practice. Lack of practice inhibits their reading comprehension. Readers’ abilities to effectively comprehend texts are significantly affected by their proficiency in accurate and automatic word recognition and prosody (May, 1998; Stanovich, 1993/1994; LaBerge & Samuels, 1974; Schreiber, 1991. Repeated reading practice has been shown to be a powerful way to improve these important fluency competencies. Certain texts are particularly well suited for repeated reading that improves both aspects of fluency

  11. Alternative Text Types to Improve Reading Fluency for Competent to Struggling Readers

    Science.gov (United States)

    Rasinski, Timothy V.; Rupley, William H.; Pagie, David D.; Nichols, William Dee

    2016-01-01

    This article offers instructional suggestions and strategies based on research and theoretical literature for developing reading fluency through the use of rhyming poetry and other texts beyond the narrative and informational texts that have been traditionally used for reading instruction. Readers' lack of fluency in reading can be a monumental…

  12. Slip of grip of a molecular motor on a crowded track: Modeling shift of reading frame of ribosome on RNA template

    Science.gov (United States)

    Mishra, Bhavya; Schütz, Gunter M.; Chowdhury, Debashish

    2016-06-01

    We develop a stochastic model for the programmed frameshift of ribosomes synthesizing a protein while moving along a mRNA template. Normally the reading frame of a ribosome decodes successive triplets of nucleotides on the mRNA in a step-by-step manner. We focus on the programmed shift of the ribosomal reading frame, forward or backward, by only one nucleotide which results in a fusion protein; it occurs when a ribosome temporarily loses its grip to its mRNA track. Special “slippery” sequences of nucleotides and also downstream secondary structures of the mRNA strand are believed to play key roles in programmed frameshift. Here we explore the role of an hitherto neglected parameter in regulating -1 programmed frameshift. Specifically, we demonstrate that the frameshift frequency can be strongly regulated also by the density of the ribosomes, all of which are engaged in simultaneous translation of the same mRNA, at and around the slippery sequence. Monte Carlo simulations support the analytical predictions obtained from a mean-field analysis of the stochastic dynamics.

  13. An intronic open reading frame was released from one of group II introns in the mitochondrial genome of the haptophyte Chrysochromulina sp. NIES-1333.

    Science.gov (United States)

    Nishimura, Yuki; Kamikawa, Ryoma; Hashimoto, Tetsuo; Inagaki, Yuji

    2014-01-01

    Mitochondrial (mt) genome sequences, which often bear introns, have been sampled from phylogenetically diverse eukaryotes. Thus, we can anticipate novel insights into intron evolution from previously unstudied mt genomes. We here investigated the origins and evolution of three introns in the mt genome of the haptophyte Chrysochromulina sp. NIES-1333, which was sequenced completely in this study. All the three introns were characterized as group II, on the basis of predicted secondary structure, and the conserved sequence motifs at the 5' and 3' termini. Our comparative studies on diverse mt genomes prompt us to propose that the Chrysochromulina mt genome laterally acquired the introns from mt genomes in distantly related eukaryotes. Many group II introns harbor intronic open reading frames for the proteins (intron-encoded proteins or IEPs), which likely facilitate the splicing of their host introns. However, we propose that a "free-standing," IEP-like protein, which is not encoded within any introns in the Chrysochromulina mt genome, is involved in the splicing of the first cox1 intron that lacks any open reading frames.

  14. The CPT1C 5'UTR contains a repressing upstream open reading frame that is regulated by cellular energy availability and AMPK.

    Directory of Open Access Journals (Sweden)

    Ines Lohse

    Full Text Available BACKGROUND: Translational control is utilized as a means of regulating gene expression in many species. In most cases, posttranscriptional regulatory mechanisms play an important role in stress response pathways and can lead to dysfunctional physiology if blocked by mutations. Carnitine Palmitoyltransferase 1 C (CPT1C, the brain-specific member of the CPT 1 family, has previously been shown to be involved in regulating metabolism in situations of energy surplus. PRINCIPAL FINDINGS: Sequence analysis of the CPT1C mRNA revealed that it contains an upstream open reading frame (uORF in the 5' UTR of its mRNA. Using CPT1C 5' UTR/luciferase constructs, we investigated the role of the uORF in translational regulation. The results presented here show that translation from the CPT1C main open reading frame (mORF is repressed by the presence of the uORF, that this repression is relieved in response to specific stress stimuli, namely glucose deprivation and palmitate-BSA treatment, and that AMPK inhibition can relieve this uORF-dependent repression. SIGNIFICANCE: The fact that the mORF regulation is relieved in response to a specific set of stress stimuli rather than general stress response, hints at an involvement of CPT1C in cellular energy-sensing pathways and provides further evidence for a role of CPT1C in hypothalamic regulation of energy homeostasis.

  15. Slip of grip of a molecular motor on a crowded track: Modeling shift of reading frame of ribosome on RNA template

    CERN Document Server

    Mishra, Bhavya; Chowdhury, Debashish

    2016-01-01

    We develop a stochastic model for the programmed frameshift of ribosomes synthesizing a protein while moving along a mRNA template. Normally the reading frame of a ribosome decodes successive triplets of nucleotides on the mRNA in a step-by-step manner. We focus on the programmed shift of the ribosomal reading frame, forward or backward, by only one nucleotide which results in a fusion protein; it occurs when a ribosome temporarily loses its grip to its mRNA track. Special "slippery" sequences of nucleotides and also downstream secondary structures of the mRNA strand are believed to play key roles in programmed frameshift. Here we explore the role of an hitherto neglected parameter in regulating -1 programmed frameshift. Specifically, we demonstrate that the frameshift frequency can be strongly regulated also by the density of the ribosomes, all of which are engaged in simultaneous translation of the same mRNA, at and around the slippery sequence. Monte Carlo simulations support the analytical predictions obt...

  16. The Reading Response E-Journal: An Alternative Way to Engage Low-Achieving EFL Students

    Science.gov (United States)

    Lee, Hsiao-Chien

    2013-01-01

    The reading response journal has been valued as an effective tool for involving students in authentic reading and writing activities. As the internet has become an essential medium in today's English classroom, it is advisable to integrate both the web and the journal when approaching our EFL students. In order to motivate and engage my…

  17. Expression of four genes of bacteriophage MB78 from contiguous open reading frames: the genomic organization as deduced by sequence analysis.

    Science.gov (United States)

    Sharma, R; Datta, P; Chakravorty, M

    2000-01-01

    Four proteins of bacteriophage MB78 having apparent molecular weights as 35, 14, 21 and 16 kDa are expressed from 3.9 kb SalI-HindIII fragment located almost in the middle of the phage genome. Analysis of the sequence supported by some experimental evidences suggest that these four proteins are expressed from polycistronic message without any intercistronic gap. Stop and start codons of consecutive ORFs overlap and rare initiation codons are used. Computer analysis of the sequence suggests the presence of two more open reading frames within the ORFs of 35 and 16 kDa proteins but in the opposite orientation, i.e. in the complementary strand.

  18. Complementation of a phycocyanin-bilin lyase from Synechocystis sp. PCC 6803 with a nucleomorph-encoded open reading frame from the cryptophyte Guillardia theta

    Directory of Open Access Journals (Sweden)

    Nyalwidhe Julius

    2008-05-01

    Full Text Available Abstract Background Cryptophytes are highly compartmentalized organisms, expressing a secondary minimized eukaryotic genome in the nucleomorph and its surrounding remnant cytoplasm, in addition to the cell nucleus, the mitochondrion and the plastid. Because the members of the nucleomorph-encoded proteome may contribute to essential cellular pathways, elucidating nucleomorph-encoded functions is of utmost interest. Unfortunately, cryptophytes are inaccessible for genetic transformations thus far. Therefore the functions of nucleomorph-encoded proteins must be elucidated indirectly by application of methods in genetically accessible organisms. Results Orf222, one of the uncharacterized nucleomorph-specific open reading frames of the cryptophyte Guillardia theta, shows homology to slr1649 of Synechocystis sp. PCC 6803. Recently a further homolog from Synechococcus sp. PCC 7002 was characterized to encode a phycocyanin-β155-bilin lyase. Here we show by insertion mutagenesis that the Synechocystis sp. PCC 6803 slr1649-encoded protein also acts as a bilin lyase, and additionally contributes to linker attachment and/or stability of phycobilisomes. Finally, our results indicate that the phycocyanin-β155-bilin lyase of Synechocystis sp. PCC 6803 can be complemented in vivo by the nucleomorph-encoded open reading frame orf222. Conclusion Our data show that the loss of phycocyanin-lyase function causes pleiotropic effects in Synechocystis sp. PCC 6803 and indicate that after separating from a common ancestor protein, the phycoerythrin lyase from Guillardia theta has retained its capacity to couple a bilin group to other phycobiliproteins. This is a further, unexpected example of the universality of phycobiliprotein lyases.

  19. Open reading frame sequencing and structure-based alignment of polypeptides encoded by RT1-Bb, RT1-Ba, RT1-Db, and RT1-Da alleles.

    Science.gov (United States)

    Ettinger, Ruth A; Moustakas, Antonis K; Lobaton, Suzanne D

    2004-11-01

    MHC class II genes are major genetic components in rats developing autoimmunity. The majority of rat MHC class II sequencing has focused on exon 2, which forms the first external domain. Sequence of the complete open reading frame for rat MHC class II haplotypes and structure-based alignment is lacking. Herein, the complete open reading frame for RT1-Bbeta, RT1-Balpha, RT1-Dbeta, and RT1-Dalpha was sequenced from ten different rat strains, covering eight serological haplotypes, namely a, b, c, d, k, l, n, and u. Each serological haplotype was unique at the nucleotide level of the sequenced RT1-B/D region. Within individual genes, the number of alleles identified was seven, seven, six, and three and the degree of amino-acid polymorphism between allotypes for each gene was 22%, 16%, 19%, and 0.4% for RT1-Bbeta, RT1-Balpha, RT1-Dbeta, and RT1-Dalpha, respectively. The extent and distribution of amino-acid polymorphism was comparable with mouse and human MHC class II. Structure-based alignment identified the beta65-66 deletion, the beta84a insertion, the alpha9a insertion, and the alpha1a-1c insertion in RT1-B previously described for H2-A. Rat allele-specific deletions were found at RT1-Balpha76 and RT1-Dbeta90-92. The mature RT1-Dbeta polypeptide was one amino acid longer than HLA-DRB1 due to the position of the predicted signal peptide cleavage site. These data are important to a comprehensive understanding of MHC class II structure-function and for mechanistic studies of rat models of autoimmunity.

  20. Sequence analysis of a 9873 bp fragment of the left arm of yeast chromosome XV that contains the ARG8 and CDC33 genes, a putative riboflavin synthase beta chain gene, and four new open reading frames.

    Science.gov (United States)

    Casas, C; Aldea, M; Casamayor, A; Lafuente, M J; Gamo, F J; Gancedo, C; Ariño, J; Herrero, E

    1995-09-15

    The DNA sequence of a 9873 bp fragment located near the left telomere of chromosome XV has been determined. Sequence analysis reveals seven open reading frames. One is the ARG8 gene coding for N-acetylornithine aminotransferase. Another corresponds to CDC33, which codes for the initiation factor 4E or cap binding protein. The open reading frame AOE169 can be considered as the putative gene for the Saccharomyces cerevisiae riboflavin synthase beta chain, since its translation product shows strong homology with four prokaryotic riboflavin synthase beta chains.

  1. The segment-to-frame association in word reading: early effects of the interaction between segmental and suprasegmental information.

    Science.gov (United States)

    Sulpizio, Simone; Job, Remo

    2015-01-01

    In four reading aloud experiments we investigated the operations occurring at the level of the phonological buffer by manipulating stress and phoneme information. In all experiments we adopted a masked priming paradigm with three-syllable Italian word targets. Experiments 1 and 2 tested the effect of pure segmental (e.g., fe%%%% - FEcola) and pure suprasegmental (CInema - FEcola) overlap, respectively. Experiments 3 and 4 tested the joint manipulation of segmental and suprasegmental information, by using prime-target pairs that shared the first syllable and did or did not share their stress pattern (e.g., FEgato - FEcola vs. feNIce - FEcola). The results showed that both segmental and suprasegmental primes affect reading at an abstract phonological level. Moreover, the joint manipulation of stress and phonemes showed an asymmetric pattern for different stress patterns, suggesting that the phonemic and the stress systems address the articulation planning through a process that starts as soon as the relevant information about the to-be-planned unit is active.

  2. The segment-to-frame association in word reading: Early effects of the interaction between segmental and suprasegmental information

    Directory of Open Access Journals (Sweden)

    Simone eSulpizio

    2015-10-01

    Full Text Available In four reading aloud experiments we investigated the operations occurring at the level of the phonological buffer by manipulating stress and phoneme information. In all experiments we adopted a masked priming paradigm with three-syllable Italian word targets. Experiments 1 and 2 tested the effect of pure segmental (e.g., fe%%%% – FEcola and pure suprasegmental (CInema – FEcola overlap, respectively. Experiments 3 and 4 tested the joint manipulation of segmental and suprasegmental information, by using prime-target pairs that shared the first syllable and did or did not share their stress pattern (e.g. FEgato – FEcola vs. feNIce – FEcola. The results showed that both segmental and suprasegmental primes affect reading at an abstract phonological level. Moreover, the joint manipulation of stress and phonemes showed an asymmetric pattern for different stress patterns, suggesting that the phonemic and the stress systems address the articulation planning through a process that starts as soon as the relevant information about the to-be-planned unit is active.

  3. Alternate Reading Strategies and Variable Asymmetry of the Planum Temporale in Adult Resilient Readers

    Science.gov (United States)

    Welcome, Suzanne E.; Leonard, Christiana M.; Chiarello, Christine

    2010-01-01

    Resilient readers are characterized by impaired phonological processing despite skilled text comprehension. We investigated orthographic and semantic processing in resilient readers to examine mechanisms of compensation for poor phonological decoding. Performance on phonological (phoneme deletion, pseudoword reading), orthographic (orthographic…

  4. Generation of Ips cells using defined factors linked via the self-cleaving 2A sequences in a single open reading frame

    Institute of Scientific and Technical Information of China (English)

    Lijian Shao; Wei Feng; Yan Sun; Hao Bai; Jun Liu; Caroline Currie; Jaejung Kim; Rafael Gama; Zack Wang; Zhijian Qian; Lucy Liaw; Wen-Shu Wu

    2009-01-01

    Generation of induced pluripotent stem (iPS) cells from somatic cells has been achieved successfully by simultane-ous viral transduction of defined reprogramming transcription factors (TFs). However, the process requires multiple viral vectors for gene delivery. As a result, generated iPS cells harbor numerous viral integration sites in their ge-nomes. This can increase the probability of gene mutagenesis and genomic instability, and present significant barriers to both research and clinical application studies of iPS cells. In this paper, we present a simple lentivirus reprogram-ming system in which defined factors are fused in-frame into a single open reading frame (ORF) via self-cleaving 2A sequences. A GFP marker is placed downstream of the transgene to enable tracking of transgene expression. We demonstrate that this polycistronic expression system efficiently generates iPS cells. The generated iPS cells have nor-mal karyotypes and are similar to mouse embryonic stem cells in morphology and gene expression. Moreover, they can differentiate into cell types of the three embryonic germ layers in both in vitro and in vivo assays. Remarkably, most of these iPS cells only harbor a single copy of viral vector. This system provides a valuable tool for generation of iPS cells, and our data suggest that the balance of expression of transduced reprogramming TFs in each cell is essen-tial for the reprogramming process. More importantly, when delivered by non-integrating gene-delivery systems, this re-engineered single ORF will facilitate efficient generation of human iPS cells free of genetic modifications.

  5. tsORFdb: theoretical small open reading frames (ORFs) database and massProphet: peptide mass fingerprinting (PMF) tool for unknown small functional ORFs.

    Science.gov (United States)

    Heo, Hyoung-Sam; Lee, Sanghyuk; Kim, Ji Min; Choi, Yeon Ja; Chung, Hae Young; Oh, S June

    2010-06-18

    Peptide mass fingerprinting (PMF) has become one of the most widely used methods for rapid identification of proteins in proteomics research. Many peaks, however, remain unassigned after PMF analysis, partly because of post-translational modification and the limited scope of protein sequences. Almost all PMF tools employ only known or predicted protein sequences and do not include open reading frames (ORFs) in the genome, which eliminates the chance of finding novel functional peptides. Unlike most tools that search protein sequences from known coding sequences, the tool we developed uses a database for theoretical small ORFs (tsORFs) and a PMF application using a tsORFs database (tsORFdb). The tsORFdb is a database for ORFeome that encompasses all potential tsORFs derived from whole genome sequences as well as the predicted ones. The massProphet system tries to extend the search scope to include the ORFeome using the tsORFdb. The tsORFdb and massProphet should be useful for proteomics research to give information about unknown small ORFs as well as predicted and registered proteins.

  6. Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene.

    Science.gov (United States)

    Köhler, R H; Horn, R; Lössl, A; Zetsche, K

    1991-07-01

    The organization and expression of the mitochondrial (mt) genome of fertile, male-sterile and restored lines of Helianthus annuus and of H. petiolaris were compared to identify alterations which might lead to cytoplasmic male sterility (CMS). The mtDNAs of fertile and male-sterile lines differ by an 11 kb inversion and a 5 kb insertion. The rearrangements seem to be the result of recombination events within an inverted repeat of 261 bp. Detectable alterations in the transcript pattern of the rearranged mtDNA regions are restricted to the atpA locus. The male-sterile line CMSBaso shows three additional transcripts of the atpA locus of about 2500, 1200 and 250 nucleotides which are not detectable in Baso. However, the coding sequences of the atpA gene are entirely identical in the fertile line Baso and the male-sterile line CMSBaso. But a new open reading frame (orfH522) of 522 nucleotides is co-transcribed with the atpA gene as an additional larger transcript of about 2500 nucleotides in CMSBaso. orfH522 is also included in a second additional transcript of about 1200 nucleotides. The predicted translation product of orfH522 might play a role in CMS in sunflower.

  7. The nucleotide sequence of a 39 kb segment of yeast chromosome IV: 12 new open reading frames, nine known genes and one genes for Gly-tRNA.

    Science.gov (United States)

    Bahr, A; Möller-Rieker, S; Hankeln, T; Kraemer, C; Protin, U; Schmidt, E R

    1997-02-01

    The complete nucleotide sequence of a 39,090 bp segment from the left arm of yeast chromosome IV was determined. Twenty-one open reading frames (ORFs) longer than 100 amino acids and a Gly-tRNA gene were discovered. Nine of the 21 ORFs (D0892, D1022, D1037, D1045, D1057, D1204, D1209, D1214, D1219) correspond to the previously sequenced Saccharomyces cerevisiae genes for the NAD-dependent glutamate dehydrogenase (GDH), the secretory component (SHR3), the GABA transport protein (UGA4), the high mobility group-like protein (NHP2), the hydroxymethylbilane synthase (HEM3), the methylated DNA protein-cysteine S-methyltransferase (MGT1), a putative sugar transport protein, the Shm1 protein (SHM1) and the anti-silencing protein (ASF2). The inferred amino acid sequences of 11 ORFs show significant similarity with known proteins from various organisms, whereas the remaining ORF does not share any similarity with known proteins.

  8. Basic functional analysis of six unknown open reading frames from Saccharomyces cerevisiae: four from chromosome VII and two from chromosome XV.

    Science.gov (United States)

    Pearson, Bruce M; Schweizer, Michael

    2002-01-30

    Six open reading frames (ORFs) of unknown function from Saccharomyces cerevisiae from the left arms of chromosomes VII and XV were disrupted by the short-flanking homology method in the diploid strains FY1679 and CENPK2. In each case, the entire ORF, with the exception of the first nucleotide of the start codon, was eliminated and replaced by the kanMX4 cassette. Correct integration of the disrupting marker was checked by colony PCR of the geneticin (G418)-resistant transformants. Sporulation followed by tetrad dissection of the diploids revealed that none of the ORFs encoded a product essential for the viability of either yeast strain. The neutral effect of these disruptions extended to mating and sporulation, since it was possible to create homozygous diploid disruptants that were capable of sporulation. Basic phenotypic analysis was carried out on all strains by growing them on three different media at three different temperatures and revealed no significant differences between disruptants and the parental strains. A cognate clone and a kanMX4 disruption cassette were created for five of the six ORFs by gap repair with specific long-flanking homology cassettes. For experimental reasons, the cognate clone and disruption cassette corresponding to the sixth ORF (YGL161w) had to be created by PCR.

  9. A cis-acting element present within the Gag open reading frame negatively impacts on the activity of the HIV-1 IRES.

    Directory of Open Access Journals (Sweden)

    Fernando Valiente-Echeverría

    Full Text Available Translation initiation from the human immunodeficiency virus type-1 (HIV-1 mRNA can occur through a cap or an IRES dependent mechanism. Cap-dependent translation initiation of the HIV-1 mRNA can be inhibited by the instability element (INS-1, a cis-acting regulatory element present within the gag open reading frame (ORF. In this study we evaluated the impact of the INS-1 on HIV-1 IRES-mediated translation initiation. Using heterologous bicistronic mRNAs, we show that the INS-1 negatively impact on HIV-1 IRES-driven translation in in vitro and in cell-based experiments. Additionally, our results show that the inhibitory effect of the INS-1 is not general to all IRESes since it does not hinder translation driven by the HCV IRES. The inhibition by the INS-1 was partially rescued in cells by the overexpression of the viral Rev protein or hnRNPA1.

  10. Immunogenicity of a Multi-Epitope DNA Vaccine Encoding Epitopes from Cu–Zn Superoxide Dismutase and Open Reading Frames of Brucella abortus in Mice

    Science.gov (United States)

    Escalona, Emilia; Sáez, Darwin; Oñate, Angel

    2017-01-01

    Brucellosis is a bacterial zoonotic disease affecting several mammalian species that is transmitted to humans by direct or indirect contact with infected animals or their products. In cattle, brucellosis is almost invariably caused by Brucella abortus. Live, attenuated Brucella vaccines are commonly used to prevent illness in cattle, but can cause abortions in pregnant animals. It is, therefore, desirable to design an effective and safer vaccine against Brucella. We have used specific Brucella antigens that induce immunity and protection against B. abortus. A novel recombinant multi-epitope DNA vaccine specific for brucellosis was developed. To design the vaccine construct, we employed bioinformatics tools to predict epitopes present in Cu–Zn superoxide dismutase and in the open reading frames of the genomic island-3 (BAB1_0260, BAB1_0270, BAB1_0273, and BAB1_0278) of Brucella. We successfully designed a multi-epitope DNA plasmid vaccine chimera that encodes and expresses 21 epitopes. This DNA vaccine induced a specific humoral and cellular immune response in BALB/c mice. It induced a typical T-helper 1 response, eliciting production of immunoglobulin G2a and IFN-γ particularly associated with the Th1 cell subset of CD4+ T cells. The production of IL-4, an indicator of Th2 activation, was not detected in splenocytes. Therefore, it is reasonable to suggest that the vaccine induced a predominantly Th1 response. The vaccine induced a statistically significant level of protection in BALB/c mice when challenged with B. abortus 2308. This is the first use of an in silico strategy to a design a multi-epitope DNA vaccine against B. abortus. PMID:28232837

  11. The varicella-zoster virus-mediated delayed host shutoff: open reading frame 17 has no major function, whereas immediate-early 63 protein represses heterologous gene expression.

    Science.gov (United States)

    Desloges, Nathalie; Rahaus, Markus; Wolff, Manfred H

    2005-12-01

    We reported that varicella-zoster virus (VZV) causes a delayed host shutoff during its replicative cycle. VZV open reading frame 17 (ORF17) is the homologue of the herpes simplex virus (HSV) UL41 gene encoding the virion host shutoff (vhs) protein which is responsible for the shutoff effect observed in HSV-infected cells. In the present study, we demonstrated that ORF17 is expressed as a late protein during the VZV replicative cycle in different infected permissive cell lines which showed a delayed shutoff of cellular RNA. A cell line with stable expression of VZV ORF17 was infected with VZV. In these cells, VZV replication and delayed host shutoff remained unchanged when compared to normal infected cells. ORF17 was not capable of repressing the expression of the beta-gal reporter gene under the control of the human cytomegalovirus immediate-early gene promoter or to inhibit the expression of a CAT reporter gene under the control of the human GAPDH promoter, indicating that ORF17 has no major function in the VZV-mediated delayed host shutoff. To determine whether other viral factors are involved in the host shutoff, a series of cotransfection assays was performed. We found that the immediate-early 63 protein (IE63) was able to downregulate the expression of reporter genes under the control of the two heterologous promoters, indicating that this viral factor can be involved in the VZV-mediated delayed host shutoff. Other factors can be also implicated to modulate the repressing action of IE63 to achieve a precise balance between the viral and cellular gene expression.

  12. Sequence-specific binding of a hormonally regulated mRNA binding protein to cytidine-rich sequences in the lutropin receptor open reading frame.

    Science.gov (United States)

    Kash, J C; Menon, K M

    1999-12-21

    In previous studies, a lutropin receptor mRNA binding protein implicated in the hormonal regulation of lutropin receptor mRNA stability was identified. This protein, termed LRBP-1, was shown by RNA gel electrophoretic mobility shift assay to specifically interact with lutropin receptor RNA sequences. The present studies have examined the specificity of lutropin receptor mRNA recognition by LRBP-1 and mapped the contact site by RNA footprinting and by site-directed mutagenesis. LRBP-1 was partially purified by cation-exchange chromatography, and the mRNA binding properties of the partially purified LRBP-1 were examined by RNA gel electrophoretic mobility shift assay and hydroxyl-radical RNA footprinting. These data showed that the LRBP-1 binding site is located between nucleotides 203 and 220 of the receptor open reading frame, and consists of the bipartite polypyrimidine sequence 5'-UCUC-X(7)-UCUCCCU-3'. Competition RNA gel electrophoretic mobility shift assays demonstrated that homoribopolymers of poly(rC) were effective RNA binding competitors, while poly(rA), poly(rG), and poly(rU) showed no effect. Mutagenesis of the cytidine residues contained within the LRBP-1 binding site demonstrated that all the cytidines in the bipartite sequence contribute to LRBP-1 binding specificity. Additionally, RNA gel electrophoretic mobility supershift analysis showed that LRBP-1 was not recognized by antibodies against two well-characterized poly(rC) RNA binding proteins, alphaCP-1 and alphaCP-2, implicated in the regulation of RNA stability of alpha-globin and tyrosine hydroxylase mRNAs. In summary, we show that partially purified LRBP-1 binds to a polypyrimidine sequence within nucleotides 203 and 220 of lutropin receptor mRNA with a high degree of specificity which is indicative of its role in posttranscriptional control of lutropin receptor expression.

  13. Mechanical Color Reading of Wood-Staining Fungal Pigment Textile Dyes: An Alternative Method for Determining Colorfastness

    Directory of Open Access Journals (Sweden)

    Eric M. Hinsch

    2016-07-01

    Full Text Available Colorfastness to washing and crocking (color loss due to rubbing are essential qualities for any dye/fabric combination that will be used for garments or upholstery. In this study, colorfastness to washing and crocking of fabrics dyed with wood-staining fungal pigments was compared to colorfastness of commercial dyes using an alternative mechanical testing method. Overall, wood-staining fungal pigments out performed commercial dyes for colorfastness to washing and wet and dry crocking. Xylindein was the most colorfast dye. Draconin red yielded inconsistent results, and the yellow pigment required a mordant to achieve any colorfastness. This study showed that the mechanical color reading method, along with statistical analysis, provided an objective, repeatable gauge of colorfastness, although visual inspection is also needed for practical purposes.

  14. Evaluation and validity of a polymerase chain reaction-based open reading frame typing method to dissect the molecular epidemiology for Acinetobacter baumannii in an epidemiologic study of a hospital outbreak.

    Science.gov (United States)

    Fujikura, Yuji; Yuki, Atsushi; Hamamoto, Takaaki; Ichimura, Sadahiro; Kawana, Akihiko; Ohkusu, Kiyofumi; Matsumoto, Tetsuya

    2016-11-01

    Acinetobacter baumannii is regarded as one of the most important pathogens in hospital outbreaks. To obtain an efficient and simple epidemiologic method of surveillance during outbreaks, we assessed the applicability of the polymerase chain reaction-based open reading frames typing (POT) method and compared it with pulsed-field gel electrophoresis. The POT method was found to have sufficient discriminatory power to identify the strains and would be widely applicable to epidemiologic surveillance during hospital outbreaks.

  15. Validation of analytical methods in GMP: the disposable Fast Read 102® device, an alternative practical approach for cell counting

    Directory of Open Access Journals (Sweden)

    Gunetti Monica

    2012-05-01

    Full Text Available Abstract Background The quality and safety of advanced therapy products must be maintained throughout their production and quality control cycle to ensure their final use in patients. We validated the cell count method according to the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use and European Pharmacopoeia, considering the tests’ accuracy, precision, repeatability, linearity and range. Methods As the cell count is a potency test, we checked accuracy, precision, and linearity, according to ICH Q2. Briefly our experimental approach was first to evaluate the accuracy of Fast Read 102® compared to the Bürker chamber. Once the accuracy of the alternative method was demonstrated, we checked the precision and linearity test only using Fast Read 102®. The data were statistically analyzed by average, standard deviation and coefficient of variation percentages inter and intra operator. Results All the tests performed met the established acceptance criteria of a coefficient of variation of less than ten percent. For the cell count, the precision reached by each operator had a coefficient of variation of less than ten percent (total cells and under five percent (viable cells. The best range of dilution, to obtain a slope line value very similar to 1, was between 1:8 and 1:128. Conclusions Our data demonstrated that the Fast Read 102® count method is accurate, precise and ensures the linearity of the results obtained in a range of cell dilution. Under our standard method procedures, this assay may thus be considered a good quality control method for the cell count as a batch release quality control test. Moreover, the Fast Read 102® chamber is a plastic, disposable device that allows a number of samples to be counted in the same chamber. Last but not least, it overcomes the problem of chamber washing after use and so allows a cell count in a clean environment such as that in a

  16. Compliance Framing - Framing Compliance

    OpenAIRE

    Lutz-Ulrich Haack; Martin C. Reimann

    2012-01-01

    Corporations have to install various organizational measures to comply with legal as well as internal guidelines systematically. Compliance management systems have the challenging task to make use of an internal compliance-marketing approach in order to ensure not only an adequate but also effective compliance-culture. Compliance-literature and findings of persuasive goal-framing-theory give opposite implications for establishing a rather values- versus rule-based compliance-culture respectiv...

  17. Finite Operator-Valued Frames

    OpenAIRE

    Meng, Bin

    2010-01-01

    Operator-valued frames are natural generalization of frames that have been used in quantum computing, packets encoding, etc. In this paper, we focus on developing the theory about operator-valued frames for finite Hilbert spaces. Some results concerning dilation, alternate dual, and existence of operator-valued frames are given. Then we characterize the optimal operator-valued frames under the case which one packet of data is lost in transmission. At last we construct the operator-valued fram...

  18. Finite Operator-Valued Frames

    CERN Document Server

    Meng, Bin

    2010-01-01

    Operator-valued frames are natural generalization of frames that have been used in quantum computing, packets encoding, etc. In this paper, we focus on developing the theory about operator-valued frames for finite Hilbert spaces. Some results concerning dilation, alternate dual, and existence of operator-valued frames are given. Then we characterize the optimal operator-valued frames under the case which one packet of data is lost in transmission. At last we construct the operator-valued frames $\\{V_j\\}_{j=1}^m$ with given frame operator $S$ and satisfying $V_jV_j^*=\\alpha_jI$, where $\\alpha_j's$ are positive numbers.

  19. Sparse Matrices in Frame Theory

    DEFF Research Database (Denmark)

    Lemvig, Jakob; Krahmer, Felix; Kutyniok, Gitta

    2014-01-01

    Frame theory is closely intertwined with signal processing through a canon of methodologies for the analysis of signals using (redundant) linear measurements. The canonical dual frame associated with a frame provides a means for reconstruction by a least squares approach, but other dual frames...... yield alternative reconstruction procedures. The novel paradigm of sparsity has recently entered the area of frame theory in various ways. Of those different sparsity perspectives, we will focus on the situations where frames and (not necessarily canonical) dual frames can be written as sparse matrices...

  20. Framing politics

    OpenAIRE

    Lecheler, S.K.

    2010-01-01

    This dissertation supplies a number of research findings that add to a theory of news framing effects, and also to the understanding of the role media effects play in political communication. We show that researchers must think more about what actually constitutes a framing effect, and that a dissociation of framing from other media effects concepts is not necessarily the ideal way to go in creating a more advanced framing theory. We also provide the first contours of a theory of news framing...

  1. Frames and semi-frames

    Energy Technology Data Exchange (ETDEWEB)

    Antoine, Jean-Pierre [Institut de Recherche en Mathematique et Physique, Universite Catholique de Louvain, B-1348 Louvain-la Neuve (Belgium); Balazs, Peter, E-mail: jean-pierre.antoine@uclouvain.be, E-mail: peter.balazs@oeaw.ac.at [Acoustics Research Institute, Austrian Academy of Sciences, Wohllebengasse 12-14, A-1040 Vienna (Austria)

    2011-05-20

    Loosely speaking, a semi-frame is a generalized frame for which one of the frame bounds is absent. More precisely, given a total sequence in a Hilbert space, we speak of an upper (resp. lower) semi-frame if only the upper (resp. lower) frame bound is valid. Equivalently, for an upper semi-frame, the frame operator is bounded, but has an unbounded inverse, whereas a lower semi-frame has an unbounded frame operator, with a bounded inverse. We study mostly upper semi-frames, both in the continuous and discrete case, and give some remarks for the dual situation. In particular, we show that reconstruction is still possible in certain cases.

  2. A FRAME response to the Draft Report on Alternative (Non-animal) Methods for Cosmetics Testing: Current Status and Future Prospects--2010.

    Science.gov (United States)

    Balls, Michael; Clothier, Richard

    2010-10-01

    This response on behalf of FRAME to the European Commission's consultation on the five chapters of the Draft Report on Alternative (Non-animal) Methods for Cosmetics Testing: Current Status and Future Prospects--2010, is via a Comment in ATLA, rather than via the template supplied by the Commission. This is principally so that a number of general points about cosmetic ingredient testing can be made. It is concluded that the five draft chapters do not provide a credible basis for the Commission's forthcoming report to the European Parliament and the European Council on the five cosmetic ingredient safety issues for which the 7th Amendment to the Cosmetic Directive's ban on animal testing was postponed until 2013. This is mainly because there is insufficient focus in the draft chapters on the specific nature of cosmetic ingredients, their uses, their local effects and metabolism at their sites of application, and, in particular, on whether their possible absorption into the body would be likely to lead to their accumulation in target sites at levels approaching Thresholds of Toxicological Concern. Meanwhile, there continues to be uncertainty about how the provisions of the Cosmetics Directive should be applied, given the requirements of the REACH system and directives concerned with the safety of other chemicals and products.

  3. Alternative strategies for the reading EFL classroom. Estrategias Alternativas para el aula de lectura de Inglés como Lengua Extranjera.

    OpenAIRE

    Suescún Giraldo, Claudia Marcela

    2015-01-01

    AbstractThis article presents the results of a research project which objective was to analyze the impact of a program with alternative reading strategies for EFL development. This research pretends to cast new lights in the constant effort from both EFLteachers and teacher trainers to promote reading among their students, through the implementation and analysis of some specific strategies, applied on a group of students of the B.A in EFL Teaching from Fundación Universitaria Luis Amigó, focu...

  4. Sequence and functional analysis of a 7.2 kb DNA fragment containing four open reading frames located between RPB5 and CDC28 on the right arm of chromosome II.

    Science.gov (United States)

    Rose, M; Kiesau, P; Proft, M; Entian, K D

    1995-07-01

    In a coordinated approach, several laboratories sequenced Saccharomyces cerevisiae chromosome II during the European BRIDGE project. Here we report on the sequence and functional analysis of a 7217 bp fragment located on the right arm of chromosome II between RPB5 and CDC28. The fragment contains four open reading frames probably encoding proteins of 79.2 kDa (corresponding gene YBR156c), 12.1 kDa (YBR157c), 62.7 kDa (YBR158w) and 38.7 kDa (YBR159w). All four open reading frames encode new proteins, as concluded from data base searches. The respective genes were destroyed by gene replacement in one allele of diploid cells. After sporulation and tetrad analysis, the resulting mutant haploid strains were investigated. No phenotype with respect to spore germination, viability, carbohydrate utilization, and growth was found for YBR157c, encoding the smallest open reading frame investigated. Gene replacement within the YBR156c gene encoding a highly basic and possibly nuclear located protein was lethal. Ybr158 revealed similarities to the Grrl (Cat80) protein with respect to the leucine-rich region. Cells harboring a mutation in the YBR158w gene showed strongly reduced growth as compared to the wild-type cells. The protein predicted from YBR159w shared 33% identical amino acid residues with the human estradiol 17-beta-hydroxysterol dehydrogenase 3. Haploid ybr159c mutants were only able to grow at reduced temperatures, but even under these conditions the mutants grew slower than wild-type strains.

  5. Open reading frame 5 (ORF5), encoding a ferredoxinlike protein, and nifQ are cotranscribed with nifE, nifN, nifX, and ORF4 in Rhodobacter capsulatus.

    OpenAIRE

    Moreno-Vivian, C; Hennecke, S; Pühler, A.; Klipp, W

    1989-01-01

    DNA sequence analysis of a 1,600-base-pair fragment located downstream of nifENX in nif region A of Rhodobacter capsulatus revealed two additional open reading frames (ORFs): ORF5, encoding a ferredoxinlike protein, and nifQ. The ferredoxinlike gene product contained two cysteine motifs, typical of ferredoxins coordinating two 4Fe-4S clusters, but the distance between these two motifs was unusual for low-molecular-weight ferredoxins. The R. capsulatus nifQ gene product shared a high degree of...

  6. The sequence of an 11.1 kb fragment on the left arm of Saccharomyces cerevisiae chromosome VII reveals six open reading frames including NSP49, KEM1 and four putative new genes.

    Science.gov (United States)

    Bertani, I; Coglievina, M; Zaccaria, P; Klima, R; Bruschi, C V

    1995-09-30

    We report the sequence of an 11.1 kb fragment located on the left arm of chromosome VII of Saccharomyces cerevisiae. By sequence analysis we have detected six open reading frames (ORFs) longer that 300 bp, which cover 87% of the entire sequence. ORF G1645 is 100% identical to the KEM1 gene, also identified as DST2, XRN1, SEP1 and RAR5, while G1648 is 100% identical to the NSP49 or NUP49 gene. ORF G1642 shares some identity with a hypothetical protein of Caenorhabditis elegans, while the other four ORFs show no significant homology to known proteins.

  7. The sequence of a 8 kb segment on the right arm of yeast chromosome VII identifies four new open reading frames and the genes for yTAFII145.

    Science.gov (United States)

    Ruzzi, M; Marconi, A; Saliola, M; Fabiani, L; Montebove, F; Frontali, L

    1997-03-30

    We report the sequence of a 8,061 bp fragment of Saccharomyces cerevisiae chromosome VII. Five open reading frames (ORFs) of at least 100 amino acids were identified. Three show similarities to the amino-acid sequence of known gene products. ORF G9374 corresponds to the gene coding for the yTAFII145 protein: a TBP-associated factor whose amino-acid sequence was previously reported (Reese et al., 1994). The remaining ORF does not display similarities to known sequences.

  8. TRANSLATION START SITE MULTIPLICITY OF THE CCAAT ENHANCER-BINDING PROTEIN-ALPHA MESSENGER-RNA IS DICTATED BY A SMALL 5' OPEN READING FRAME

    NARCIS (Netherlands)

    CALKHOVEN, CF; BOUWMAN, PRJ; SNIPPE, L; AB, G

    1994-01-01

    The CCAAT/enhancer binding proteins (C/EBP) alpha and beta of the bZIP family of transcription factors each occur as multiple forms due to translation initiation at different in-frame AUG codons from the same messenger RNA. The C/EBP alpha mRNAs of chicken, rat and Xenopus all contain a small 5' ope

  9. Framing politics

    NARCIS (Netherlands)

    Lecheler, S.K.

    2010-01-01

    This dissertation supplies a number of research findings that add to a theory of news framing effects, and also to the understanding of the role media effects play in political communication. We show that researchers must think more about what actually constitutes a framing effect, and that a dissoc

  10. Framing theory

    NARCIS (Netherlands)

    de Vreese, C.H.; Lecheler, S.; Mazzoleni, G.; Barnhurst, K.G.; Ikeda, K.; Maia, R.C.M.; Wessler, H.

    2016-01-01

    Political issues can be viewed from different perspectives and they can be defined differently in the news media by emphasizing some aspects and leaving others aside. This is at the core of news framing theory. Framing originates within sociology and psychology and has become one of the most used th

  11. Fusion Frames and -Frames in Banach Spaces

    Indian Academy of Sciences (India)

    Amir Khosravi; Behrooz Khosravi

    2011-05-01

    Fusion frames and -frames in Hilbert spaces are generalizations of frames, and frames were extended to Banach spaces. In this article we introduce fusion frames, -frames, Banach -frames in Banach spaces and we show that they share many useful properties with their corresponding notions in Hilbert spaces. We also show that -frames, fusion frames and Banach -frames are stable under small perturbations and invertible operators.

  12. Sequence analysis of a 14.2 kb fragment of Saccharomyces cerevisiae chromosome XIV that includes the ypt53, tRNALeu and gsr m2 genes and four new open reading frames.

    Science.gov (United States)

    Garcia-Cantalejo, J M; Boskovic, J; Jimenez, A

    1996-05-01

    As part of the EU yeast genome program, a fragment of 14,262 bp from the left arm of Saccharomyces cerevisiae chromosome XIV has been sequenced. This fragment corresponds to cosmid 14-14b and is located roughly 130 kb from the centromere. It contains four new open reading frames which encode potential proteins of more than 99 amino acids, as well as the ypt53, tRNALeu and gsr moffenes. The putative protein N2212 is similar to the ribosomal protein S7 from humans. N2215 contains several predicted transmembrane elements. N2231 contains regions which are rich in acidic, as well as basic, residues which could from alpha-helical structures. Similar regions are found in a variety of proteins including glutamic acid rich protein, trichohyalin, caldesmon, Tb-29 and several cytoskeleton-interacting proteins.

  13. An 18.3 kb DNA fragment from yeast chromosome VII carries four unknown open reading frames, the gene for an Asn synthase, remnants of Ty and three tRNA genes.

    Science.gov (United States)

    Van Dyck, L; Tettelin, H; Purnelle, B; Goffeau, A

    1997-02-01

    An 18.3 kb DNA segment from yeast Saccharomyces cerevisiae VII encompasses the previously characterized MEP1, NUP57 and PPT1 genes as well as seven new open reading frames (ORFs) of at least 100 residues. G6358 is an ubiquitous glutamine-dependent asparagine synthase. G6362 is membrane protein highly homologous to a protein of unknown function in the yeast Schizosaccharomyces pombe. Three ORFs (G6324, G6335 and G6365) have no significant homology with previously reported proteins of characteristic motifs. G6321 and G6359, enclosed in longer ORFs, are not likely to be coding. The segment also contains tRNA genes for Asn, Arg and Ile as well as sigma element and two solo deltas. ORFs and genetic elements are named according to a preliminary working nomenclature.

  14. Physiological Expression and Accumulation of the Products of Two Upstream Open Reading Frames mrtl and MycHex1 Along With p64 and p67 Myc From the Human c-myc Locus.

    Science.gov (United States)

    Ji, Mi Hong; Kim, Seung-Ki; Kim, Chae-Yong; Phi, Ji Hoon; Jun, Hyun Jin; Blume, Scott W; Choi, Hyoung Soo

    2016-06-01

    In addition to the canonical c-Myc p64 and p67 proteins, the human c-myc locus encodes two distinct proteins, mrtl (myc-related translation/localization regulatory factor) and MycHex1 (Myc Human Exon 1), from the upstream open reading frames within the 5'-untranslated region of the c-myc P0 mRNA. The aim of this study is to examine simultaneously, for the first time, mrtl, MycHex1, c-Myc p64, and p67 in human tumor cell lines and pediatric brain tumor tissues. Western blot analysis demonstrated endogenous mrtl, MycHex1, c-Myc p64, and p67 simultaneously. The relative abundance of mrtl and MycHex1 were consistent among a variety of human tumor cell lines, and the relative intensities of mrtl and MycHex1 correlated positively. Confocal imaging revealed mrtl predominantly localized to the nuclear envelope, along with prominent reticular pattern in the cytoplasm. MycHex1 was observed as a series of bright foci located within the nucleus, a subset of which colocalized with fibrillarin. mrtl and MycHex1 co-immunoprecipitated with RACK1, c-Myc, fibrillarin, coilin, and with each other. These findings suggest that mrtl and MycHex1 have multiple interaction partners in both the nucleus and cytoplasm. Sequence analyses confirmed a known polymorphism of mrtl at base 1965 (G>T) and new mutations at bases 1900 (C>G) and 1798 (C>G). Evidence is presented for expression and stable accumulation of all four proteins encoded by three distinct non-overlapping open reading frames within the human c-myc locus. Additional work is warranted to further elucidate the functional or regulatory roles of these molecules in regulation of c-Myc and in oncogenesis.

  15. Production of Candida antarctica lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in an ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production.

    Science.gov (United States)

    Hughes, Stephen R; Moser, Bryan R; Harmsen, Amanda J; Bischoff, Kenneth M; Jones, Marjorie A; Pinkelman, Rebecca; Bang, Sookie S; Tasaki, Ken; Doll, Kenneth M; Qureshi, Nasib; Saha, Badal C; Liu, Siqing; Jackson, John S; Robinson, Samantha; Cotta, Michael C; Rich, Joseph O; Caimi, Paolo

    2011-02-01

    A synthetic Candida antarctica lipase B (CALB) gene open reading frame (ORF) for expression in yeast was constructed, and the lycotoxin-1 (Lyt-1) C3 variant gene ORF, potentially to improve the availability of the active enzyme at the surface of the yeast cell, was added in frame with the CALB ORF using an automated PCR assembly and DNA purification protocol on an integrated robotic workcell. Saccharomyces cerevisiae strains expressing CALB protein or CALB Lyt-1 fusion protein were first grown on 2% (w/v) glucose, producing 9.3 g/L ethanol during fermentation. The carbon source was switched to galactose for GAL1-driven expression, and the CALB and CALB Lyt-1 enzymes expressed were tested for fatty acid ethyl ester (biodiesel) production. The synthetic enzymes catalyzed the formation of fatty acid ethyl esters from ethanol and either corn or soybean oil. It was further demonstrated that a one-step-charging resin, specifically selected for binding to lipase, was capable of covalent attachment of the CALB Lyt-1 enzyme, and that the resin-bound enzyme catalyzed the production of biodiesel. High-level expression of lipase in an ethanologenic yeast strain has the potential to increase the profitability of an integrated biorefinery by combining bioethanol production with coproduction of a low-cost biocatalyst that converts corn oil to biodiesel.

  16. Discussion on college students' English Reading Skills —intensive reading and extensive reading

    Institute of Scientific and Technical Information of China (English)

    敖登

    2016-01-01

    Students are exposed to a growing amount of vocabularies and knowledge as they step into higher grades, while seem to have little improvement in their reading ability and no alternative means to realize their reading goals. Improving the English reading skills is very important for students as reading enjoys a huge ratio in an English exam paper. Talking about reading, we can not ignore these facts: reading materials, reading speed and reading skills. The followings analyzed are some common skills applied to reading-intensive reading and extensive reading as well as the obstacles that may be met while reading.

  17. Timber frame walls

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place; Brandt, Erik

    2010-01-01

    A ventilated cavity is usually considered good practice for removing moisture behind the cladding of timber framed walls. Timber frame walls with no cavity are a logical alternative as they are slimmer and less expensive to produce and besides the risk of a two-sided fire behind the cladding....... It was found that the specific damages made to the vapour barrier as part of the test did not have any provable effect on the moisture content. In general elements with an intact vapour barrier did not show a critical moisture content at the wind barrier after four years of exposure....

  18. Framing the Game: Examining Frame Choice in Bargaining.

    Science.gov (United States)

    Blount; Larrick

    2000-01-01

    This article introduces the study of frame choice in negotiation. Here, the selection of a procedural frame is treated as a dependent variable-a choice that bargainers make in addition to determining their offers. The empirical focus of the article is on whether, when given a choice between two alternative versions of the ultimatum bargaining game, negotiators choose the description that maximizes their expected payoffs. For example, in one frame-choice task, negotiators assigned to the Player 1 role were asked to select between framing the game as "Player 1 proposes a division and Player 2 accepts or rejects it" or "Player 1 makes a claim from a common pool and Player 2 makes a counterclaim." Past research has shown that the second frame leads to higher expected payoffs for Player 1 than does the first. Across four studies and three established framing effects, it is found that participants consistently fail to select the procedural frames that optimize monetary outcomes. Subsequent analyses suggest that this tendency is due to two factors: (a) nonmonetary motivations, such as fairness and respect, that influence frame-choice preferences and (b) cognitive limitations that inhibit the ability to accurately predict the effect of alternative procedural frames on opponents' responses Copyright 2000 Academic Press.

  19. Expansion of Frames to Tight Frames

    Institute of Scientific and Technical Information of China (English)

    Deng Feng LI; Wen Chang SUN

    2009-01-01

    We show that every Bessel sequence (and therefore every frame) in a separable Hilbert space can be expanded to a tight frame by adding some elements. The proof is based on a recent generalization of the frame concept, the g-frame, which illustrates that g-frames could be useful in the study of frame theory. As an application, we prove that any Gabor frame can be expanded to a tight frame by adding one window function.

  20. Framing scales and scaling frames

    NARCIS (Netherlands)

    Lieshout, van M.; Dewulf, A.; Aarts, M.N.C.; Termeer, C.J.A.M.

    2009-01-01

    Policy problems are not just out there. Actors highlight different aspects of a situation as problematic and situate the problem on different scales. In this study we will analyse the way actors apply scales in their talk (or texts) to frame the complex decision-making process of the establishment o

  1. Analysis of Enzymatic Digestion Pattern of Two Open Reading Frames of Varciella–Zoster Genome from Kuwaiti Patients Using the RFLP Technique

    Directory of Open Access Journals (Sweden)

    Jafar A Qasem

    2012-12-01

    Full Text Available Background and Objectives: Varicella–Zoster virus (VZV is a human herpes virus that usually attacks young children and commonly causes chicken pox (Varicella. Following primary infection, a lifelong latent infection is established. The virus often reactivates during adulthood or senesces to cause shingles (Zoster. Little is known regarding the genotypes of Varicella in Kuwait. The aim of this study was to genotype Varicella samples collected from patients in Kuwait.Materials and Methods: Samples from 60 cases of chicken pox were typed. The DNA extraction was performed using the commercially available DNA extraction kit. Two sets of oligonucleotide primers were used to amplify the intervening sequences with polymerase chain reaction to identify VZV DNA in clinical samples. The BglI and PstI endonucleases were used to digest. The PCR amplicons for PCR-RFLP typing.Results: Relatively consistent restriction enzyme digestion profiles for different VZV strains were observed. Limited genetic differences between VZV samples were found. Three VZV strains were identified (A, B and C with type B representing 86.6%, type A 11.7% and type C being 1.7%. We found that distinct restriction fragment length polymorphism isolates from the same origin or nationality were very similar.Conclusion: Varicella strains with cutting sites for both enzyme PstI and BglI (typeB were more prevalent. Molecular amplification of viral DNA by PCR and restriction digestion could be used for VZV typing as an alternative method to serological assays.

  2. A Social Justice Alternative for Framing Post-Compulsory Education: A Human Development Perspective of VET in Times of Economic Dominance

    Science.gov (United States)

    Lopez-Fogues, Aurora

    2016-01-01

    The article provides an alternative theoretical framework for evaluating contemporary issues facing education, specifically vocational education and training (VET) in Europe. In order to accomplish this, it draws on the theoretical insights of the capability approach in the work of Amartya Sen; the concept of vulnerability as intrinsic to every…

  3. Mechanical Color Reading of Wood-Staining Fungal Pigment Textile Dyes: An Alternative Method for Determining Colorfastness

    OpenAIRE

    Eric M. Hinsch; Robinson, Sara C

    2016-01-01

    Colorfastness to washing and crocking (color loss due to rubbing) are essential qualities for any dye/fabric combination that will be used for garments or upholstery. In this study, colorfastness to washing and crocking of fabrics dyed with wood-staining fungal pigments was compared to colorfastness of commercial dyes using an alternative mechanical testing method. Overall, wood-staining fungal pigments out performed commercial dyes for colorfastness to washing and wet and dry crocking. Xylin...

  4. Reannotation of the CELO genome characterizes a set of previously unassigned open reading frames and points to novel modes of host interaction in avian adenoviruses

    Directory of Open Access Journals (Sweden)

    Washietl Stefan

    2003-11-01

    Full Text Available Abstract Background The genome of the avian adenovirus Chicken Embryo Lethal Orphan (CELO has two terminal regions without detectable homology in mammalian adenoviruses that are left without annotation in the initial analysis. Since adenoviruses have been a rich source of new insights into molecular cell biology and practical applications of CELO as gene a delivery vector are being considered, this genome appeared worth revisiting. We conducted a systematic reannotation and in-depth sequence analysis of the CELO genome. Results We describe a strongly diverged paralogous cluster including ORF-2, ORF-12, ORF-13, and ORF-14 with an ATPase/helicase domain most likely acquired from adeno-associated parvoviruses. None of these ORFs appear to have retained ATPase/helicase function and alternative functions (e.g. modulation of gene expression during the early life-cycle must be considered in an adenoviral context. Further, we identified a cluster of three putative type-1-transmembrane glycoproteins with IG-like domains (ORF-9, ORF-10, ORF-11 which are good candidates to substitute for the missing immunomodulatory functions of mammalian adenoviruses. ORF-16 (located directly adjacent displays distant homology to vertebrate mono-ADP-ribosyltransferases. Members of this family are known to be involved in immuno-regulation and similiar functions during CELO life cycle can be considered for this ORF. Finally, we describe a putative triglyceride lipase (merged ORF-18/19 with additional domains, which can be expected to have specific roles during the infection of birds, since they are unique to avian adenoviruses and Marek's disease-like viruses, a group of pathogenic avian herpesviruses. Conclusions We could characterize most of the previously unassigned ORFs pointing to functions in host-virus interaction. The results provide new directives for rationally designed experiments.

  5. Adenovirus E4 open reading frame 4-induced dephosphorylation inhibits E1A activation of the E2 promoter and E2F-1-mediated transactivation independently of the retinoblastoma tumor suppressor protein

    DEFF Research Database (Denmark)

    Mannervik, M; Fan, S; Ström, A C

    1999-01-01

    of the viral E4 open reading frame 4 (E4-ORF4) protein. This effect does not to require the retinoblastoma protein that previously has been shown to regulate E2F activity. The inhibitory activity of E4-ORF4 appears to be specific because E4-ORF4 had little effect on, for example, E4-ORF6/7 transactivation...... of the E2 promoter. We further show that the repressive effect of E4-ORF4 on E2 transcription works mainly through the E2F DNA-binding sites in the E2 promoter. In agreement with this, we find that E4-ORF4 inhibits E2F-1/DP-1-mediated transactivation. We also show that E4-ORF4 inhibits E2 mRNA expression...... during virus growth. E4-ORF4 has previously been shown to bind to and activate the cellular protein phosphatase 2A. The inhibitory effect of E4-ORF4 was relieved by okadaic acid, which inhibits protein phosphatase 2A activity, suggesting that E4-ORF4 represses E2 transcription by inducing transcription...

  6. Sequence analysis of a 13.4 kbp fragment from the left arm of chromosome XV reveals a malate dehydrogenase gene, a putative Ser/Thr protein kinase, the ribosomal L25 gene and four new open reading frames.

    Science.gov (United States)

    Casamayor, A; Khalid, H; Balcells, L; Aldea, M; Casas, C; Herrero, E; Ariño, J

    1996-09-01

    A 13421 bp fragment located near the left telomere of chromosome XV (cosmid pEOA461) has been sequenced. Seven non-overlapping open reading frames (ORFs) encoding polypeptides longer than 100 residues have been found (AOB859, AOC184, AOE375, AOX142i, AOE423, AOA476 and AOE433). An additional ORF (AOE131) is found within AOA476. Three of them (AOC184, AOA476 and AOE433) show no remarkable identity with proteins deposited in the data banks. ORF AOB859 is quite similar to a hypothetical yeast protein of similar size located in chromosome VI, particularly within the C-terminal half. AOE375 encodes a new member of the glycogen synthase kinase-3 subfamily of Ser/Thr protein kinases. AOX142i is the gene encoding the previously described ribosomal protein L25. AOE423 codes for a protein virtually identical to the MDH2 malate dehydrogenase isozyme. However, our DNA sequence shows a single one-base insertion upstream of the reported initiating codon. This would produce a larger ORF by extending 46 residues the N-terminus of the protein. The existence of this insertion has been confirmed in three different yeast strains, including FY1679.

  7. Immunogenicity and Protective Response Induced by Recombinant Plasmids Based on the BAB1_0267 and BAB1_0270 Open Reading Frames of Brucella abortus 2308 in BALB/c Mice

    Science.gov (United States)

    Gómez, Leonardo A.; Alvarez, Francisco I.; Fernández, Pablo A.; Flores, Manuel R.; Molina, Raúl E.; Coloma, Roberto F.; Oñate, Angel A.

    2016-01-01

    Immunogenicity induced by recombinant plasmids based on the BAB1_0267 and BAB1_0270 open reading frames (ORFs) of Brucella abortus 2308 was evaluated. Bioinformatics analyses indicate that the BAB1_0267 and BAB1_0270 ORFs encode a protein with a SH3 domain and a Zn-dependent metalloproteinase, respectively. Both ORFs have important effects on intracellular survival and replication of B. abortus 2308, mediated via professional and non-professional phagocytic cells. Our results show that immunization with the recombinant plasmid based on the BAB1_0267 ORF significantly increases the production of IgG1, levels of IFN-γ and the lymphoproliferative response of splenocytes. However, BAB1_0267 did not provide significant levels of protection. The plasmid based on the BAB1_0270 significantly increased IgG2a production, levels of IFN-γ and TNF-α, and the lymphoproliferative response of splenocytes. These results demonstrate that immunization with the BAB1_0270 derived recombinant plasmid induce a Th1-type immune response, correlated with a heightened resistance to B. abortus 2308 infection in mice. It is concluded that the Th1-type immune response against bacterial Zn-dependent metalloproteinase induces a protective response in mice, and that pV270 recombinant plasmid is an effective candidate microbicide against brucellosis. PMID:27747197

  8. 行星轮系的机架变换与传动比变化规律的研究%Research on the variation law of transmission ratio and frame alternation of planetary gear train

    Institute of Scientific and Technical Information of China (English)

    程爱明; 赵自强; 张春林

    2009-01-01

    The frame alternation method is an important meth-od of mechanism innovation; it has significant application prospects in the innovative design of gear train. Taking the simplest gear train as the innovative base, this paper probed into the transmission ratio relationship between the new gear train after the alternation of frame and the original gear train, thus an important conclusion was educed that the sum of the transmission ratio that takes the gear train link-ing bar as the output component and the transmission ratio of the mechanism when the linking bar was fixed equals to one. And then a certification was carried out on this conclusion. By means of car-tying out the calculations of transmission ratios on the typical plane-tary gear train, the fewer teeth differenced transmission, the transla-tional gear transmission and the frame alternation of complex hybrid gear train, the correctness of this conclusion was verified. Finally the important role of this conclusion in the calculation of transmis-sion ratio of the complex planetary gear train was clarified.%转换机架法是机构创新的重要方法,在轮系的创新设计中有重要应用前景.文中以最简单的轮系为创新基础,探讨机架变换后的新轮系与原轮系的传动比关系,得出重要结论:以轮系系杆为输出构件时的传动比与系杆固定时机构的传动比之和为1.然后对该结论进行证明,再通过典型的行星轮系、少齿差传动、平动齿轮传动和复杂的混合轮系的机架变换进行传动比的计算,验证了该结论的正确性.最后阐明了该结论在复杂行星轮系传动比计算中的重要作用.

  9. Timber frame walls

    DEFF Research Database (Denmark)

    Hansen, Ernst Jan de Place; Brandt, Erik

    2010-01-01

    A ventilated cavity is usually considered good practice for removing moisture behind the cladding of timber framed walls. Timber frame walls with no cavity are a logical alternative as they are slimmer and less expensive to produce and besides the risk of a two-sided fire behind the cladding...... were removed in some of the elements to simulate damaged vapour barriers. The condition of the wind barriers of elements with intact vapour barriers was inspected from the inside after four years of exposure. This paper presents results with emphasis on the moisture conditions behind the wind barrier....... It was found that the specific damages made to the vapour barrier as part of the test did not have any provable effect on the moisture content. In general elements with an intact vapour barrier did not show a critical moisture content at the wind barrier after four years of exposure....

  10. Effects of Problem Frame and Gender on Principals' Decision Making

    Science.gov (United States)

    Miller, Paul M.; Fagley, Nancy S.; Casella, Nancy E.

    2009-01-01

    Research indicates people's decisions can sometimes be influenced by seemingly trivial differences in the "framing" (i.e., wording) of alternative options. The tendency to prefer risk averse options when framed positively and risky options when framed negatively is known as the framing effect. The current study examined the susceptibility of…

  11. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.

    Science.gov (United States)

    Xu, Jian; Wu, Fan; Tian, Deying; Wang, Jingjing; Zheng, Zizheng; Xia, Ningshao

    2014-01-01

    Hepatitis E virus (HEV) is one of the primary causative agents of acute hepatitis, and represents a major cause of severe public health problems in developing countries. The pathogenesis of HEV is not well characterized, however, primarily due to the lack of well-defined cell and animal models. Here, we investigated the effects of genotype 1 HEV open reading frame 3 (ORF3) on TNF-α-induced nucleus factor-κappa B (NF-κB) signaling. Human lung epithelial cells (A549) were transiently transfected with ORF3 containing plasmids. These cells were then stimulated with TNF-α and the nucleus translocation of the p65 NF-κB subunit was assessed using western blot and laser confocal microscopy. DNA-binding activity of p65 was also examined using electrophoretic mobility shift assay (EMSA), and the suppression of NF-κB target genes were detected using real-time RT-PCR and ELISA. These results enabled us to identify the decreased phosphorylation levels of IKBα. We focused on the gene of negative regulation of NF-κB, represented by TNF-α-induced protein 3 (TNFAIP3, also known as A20). Reducing the levels of A20 with siRNAs significantly enhances luciferase activation of NF-κB. Furthermore, HEV ORF3 regulated A20 primarily via activating transcription factor 6 (ATF6), involved in unfolded protein response (UPR), resulting in the degradation or inactivation of the receptor interacting protein 1 (RIP1), a major upstream activator of IKB kinase compounds (IKKs). Consequently, the phosphorylation of IKBα and the nucleus translocation of p65 are blocked, which contributes to diminished NF-κB DNA-binding activation and NF-κB-dependent gene expression. The findings suggest that genotype 1 HEV, through ORF3, may transiently activate NF-κB through UPR in early stage, and subsequently inhibit TNF-α-induced NF-κB signaling in late phase so as to create a favorable virus replication environment.

  12. Open reading frame 3 of genotype 1 hepatitis E virus inhibits nuclear factor-κappa B signaling induced by tumor necrosis factor-α in human A549 lung epithelial cells.

    Directory of Open Access Journals (Sweden)

    Jian Xu

    Full Text Available Hepatitis E virus (HEV is one of the primary causative agents of acute hepatitis, and represents a major cause of severe public health problems in developing countries. The pathogenesis of HEV is not well characterized, however, primarily due to the lack of well-defined cell and animal models. Here, we investigated the effects of genotype 1 HEV open reading frame 3 (ORF3 on TNF-α-induced nucleus factor-κappa B (NF-κB signaling. Human lung epithelial cells (A549 were transiently transfected with ORF3 containing plasmids. These cells were then stimulated with TNF-α and the nucleus translocation of the p65 NF-κB subunit was assessed using western blot and laser confocal microscopy. DNA-binding activity of p65 was also examined using electrophoretic mobility shift assay (EMSA, and the suppression of NF-κB target genes were detected using real-time RT-PCR and ELISA. These results enabled us to identify the decreased phosphorylation levels of IKBα. We focused on the gene of negative regulation of NF-κB, represented by TNF-α-induced protein 3 (TNFAIP3, also known as A20. Reducing the levels of A20 with siRNAs significantly enhances luciferase activation of NF-κB. Furthermore, HEV ORF3 regulated A20 primarily via activating transcription factor 6 (ATF6, involved in unfolded protein response (UPR, resulting in the degradation or inactivation of the receptor interacting protein 1 (RIP1, a major upstream activator of IKB kinase compounds (IKKs. Consequently, the phosphorylation of IKBα and the nucleus translocation of p65 are blocked, which contributes to diminished NF-κB DNA-binding activation and NF-κB-dependent gene expression. The findings suggest that genotype 1 HEV, through ORF3, may transiently activate NF-κB through UPR in early stage, and subsequently inhibit TNF-α-induced NF-κB signaling in late phase so as to create a favorable virus replication environment.

  13. About Reading

    Institute of Scientific and Technical Information of China (English)

    独行墨客

    2004-01-01

    As for reading and for learning, reading rate (that is, words per minute, WPM) is important, especially for students who have to pass some reading test. How to compute your reading rate? You may know it after reading the following. Reading Rate (WPM) = Total number of words + reading time.

  14. Relationship between open reading frame 26 subtypes in human herpesvirns-8 and Kaposi's sarcoma%人类疱疹病毒8型ORF26基因亚型与Kaposi肉瘤的相关性研究

    Institute of Scientific and Technical Information of China (English)

    吴秀娟; 普雄明; 吴卫东

    2008-01-01

    Objective To learn the subtypes of open reading frame 26 (ORF26) of human herpesvirus 8 (HHV-8) in patients with Kaposi's sarcoma, and to evaluate the relationship of ORF26 subtypes to clinical types and invasiveness of Kaposi's sarcoma. Methods Thirty-two paraffin-embeded tissue speci-mens of Kaposi's sarcoma were collected in the Department of Dermatology, People's Hospital of Xinjiang Uygur Autonomous Region, from 1996 to 2007. DNA was extracted from these specimens, nested-PCR was used to amplify HHV-8 DNA. PCR products were subjected to bi-directional sequencing after extraction from agarose gels. Phylogenetic analysis was carded out by using the software DNAStar, program Clustal W and PHYLIP package for the determination of ORF26 subtype. Fisher's exact test was performed to evaluate the relationship of ORF26 subtypes to clinical types and invasiveness of Kaposi's sarcoma. Results Of the 32 specimens, 30 were positive for HHV-8 DNA with a positivity rate of 93.75%, and 6 specimens of AIDS related Kaposi's sarcoma were all positive. ORF26 A subtype was detected in the HHV-8 DNA of 17 posi-tive specimens, and C subtype in that of other 13 specimens. Neither the incidence of mucosal lesions nor the distribution of clinical subtypes was of significant difference between patients with ORF26 A subtype and those with ORF26 C subtype (both P > 0.05). Conclusions A and C may be the predominate subtypes of ORF 26 in HHV-8 of patients with Kaposi's sarcoma, and the ORF26 subtype is unrelated to the presence of mucosal lesions in or the clinical types of patients with Kaposi's sarcoma.%目的 明确Kaposi肉瘤患者感染的人类疱疹病毒8型(HHV-8)ORF26基因亚型分类,初步探讨其与Kaposi肉瘤不同临床分型及侵袭性的相关性.方法 对32例Kaposi肉瘤石蜡包埋组织进行HHV-8 DNA抽提、扩增、双向测序,使用DNAStar软件、Clustal W软件和PHYLIP软件包对测序结果进行系统发生学分析,从而确定HHV-8 ORF26基因亚型,

  15. Prime tight frames

    DEFF Research Database (Denmark)

    Lemvig, Jakob; Miller, Christopher; Okoudjou, Kasso A.

    2014-01-01

    to suggest effective analysis and synthesis computation strategies for such frames. Finally, we describe all prime frames constructed from the spectral tetris method, and, as a byproduct, we obtain a characterization of when the spectral tetris construction works for redundancies below two.......We introduce a class of finite tight frames called prime tight frames and prove some of their elementary properties. In particular, we show that any finite tight frame can be written as a union of prime tight frames. We then characterize all prime harmonic tight frames and use thischaracterization...

  16. Sequencing of a 9.9 kb segment on the right arm of yeast chromosome VII reveals four open reading frames, including PFK1, the gene coding for succinyl-CoA synthetase (beta-chain) and two ORFs sharing homology with ORFs of the yeast chromosome VIII.

    Science.gov (United States)

    Guerreiro, P; Azevedo, D; Barreiros, T; Rodrigues-Pousada, C

    1997-03-15

    A 9.9 kb DNA fragment from the right arm of chromosome VII of Saccharomyces cerevisiae has been sequenced and analysed. The sequence contains four open reading frames (ORFs) longer than 100 amino acids. One gene, PFK1, has already been cloned and sequenced and the other one is the probable yeast gene coding for the beta-subunit of the succinyl-CoA synthetase. The two remaining ORFs share homology with the deduced amino acid sequence (and their physical arrangement is similar to that) of the YHR161c and YHR162w ORFs from chromosome VIII.

  17. The DNA sequence of a 7941 bp fragment of the left arm of chromosome VII of Saccharomyces cerevisiae contains four open reading frames including the multicopy suppressor gene of the pop2 mutation and a putative serine/threonine protein kinase gene.

    Science.gov (United States)

    Coglievina, M; Bertani, I; Klima, R; Zaccaria, P; Bruschi, C V

    1995-06-30

    We report the sequence of a 7941 bp DNA fragment from the left arm of chromosome VII of Saccharomyces cerevisiae which contains four open reading frames (ORFs) of greater than 100 amino acid residues. ORF biC834 shows 100% bp identity with the recently identified multicopy suppressor gene of the pop2 mutation (MPT5); its deduced protein product carries an eight-repeat domain region, homologous to that found in the hypothetical regulatory YGL023 protein of S. cerevisiae and the Pumilio protein of Drosophila. ORF biE560 protein exhibits patterns typical of serine/threonine protein kinases, with which it shares high degrees of homology.

  18. Optimally Sparse Frames

    CERN Document Server

    Casazza, Peter G; Krahmer, Felix; Kutyniok, Gitta

    2010-01-01

    Frames have established themselves as a means to derive redundant, yet stable decompositions of a signal for analysis or transmission, while also promoting sparse expansions. However, when the signal dimension is large, the computation of the frame measurements of a signal typically requires a large number of additions and multiplications, and this makes a frame decomposition intractable in applications with limited computing budget. To address this problem, in this paper, we introduce sparsity of a frame as a new paradigm. In our terminology, a sparse frame is a frame whose elements have a sparse representation in an orthonormal basis, thereby enabling low-complexity frame decompositions. To introduce a precise meaning of optimality, we take the sum of the numbers of vectors needed of this orthonormal basis when expanding each frame vector as sparsity measure. We then analyze the recently introduced algorithm Spectral Tetris for construction of unit norm tight frames and prove that the tight frames generated...

  19. Message framing, it does a body good: effects of message framing and motivational orientation on young women's calcium consumption.

    Science.gov (United States)

    Gerend, Mary A; Shepherd, Melissa A

    2013-10-01

    We investigated the effects of gain-framed versus loss-framed messages and motivational orientation on calcium consumption. After completing a motivational orientation scale (behavioral inhibition system/behavioral activation system), undergraduate women (N = 141) were randomly assigned to read a gain-framed or loss-framed pamphlet promoting calcium consumption. Calcium consumption was assessed 1 month later. For calcium supplement behavior, a gain-framed advantage was observed for behavioral activation system-oriented individuals, whereas a loss-framed advantage was observed for behavioral inhibition system-oriented individuals. For dietary calcium intake, a gain-framed advantage was observed among behavioral activation system-oriented individuals; however, no framing effect emerged for behavioral inhibition system-oriented individuals. The success of framed messages depends on the message recipient's motivational orientation.

  20. Reading faster

    OpenAIRE

    Paul Nation

    2009-01-01

    This article describes the visual nature of the reading process as it relates to reading speed. It points out that there is a physical limit on normal reading speed and beyond this limit the reading process will be different from normal reading where almost every word is attended to. The article describes a range of activities for developing reading fluency, and suggests how the development of fluency can become part of a reading programme.

  1. Reading faster

    Directory of Open Access Journals (Sweden)

    Paul Nation

    2009-12-01

    Full Text Available This article describes the visual nature of the reading process as it relates to reading speed. It points out that there is a physical limit on normal reading speed and beyond this limit the reading process will be different from normal reading where almost every word is attended to. The article describes a range of activities for developing reading fluency, and suggests how the development of fluency can become part of a reading programme.

  2. Human cytomegalovirus UL144 open reading frame: sequence variability in Guangzhou congenital infected children%广州地区HCMV临床病毒株UL144 ORF的序列变异研究

    Institute of Scientific and Technical Information of China (English)

    王波; 李月琴; 叶宁; 胡兢晶; 何震宇; 田传军; 张纯青; 叶铁真; 周天鸿

    2008-01-01

    目的 研究广州地区先天性感染的人巨细胞病毒(HCMV)临床低传代分离病毒株UL144基因序列的多态性,探讨UL144基因在HCMV致病中的作用.方法 对3株经多重PCR鉴定HCMV DNA为阳性的临床低传代分离株进行HCMV UL144基因全序列PCR扩增,PCR产物克隆到pMD18-T载体上再测序,将其序列与GenBank中公布的其它10株临床分离株UL144基因一起进行分析.结果 本实验克隆并测序了HCMV临床低传代D3、D2和D52病毒株的UL144基因,提交GenBank,已被GenBank收录,序列号分别为DQ180368、DQ180382和DQ180355.HCMV临床低传代D3、D2和D52病毒株的UL144基因均全长531 bp.通过blast分析,从GenBank中找到了10株HCMV病毒株的UL144与D3、D2和D52的UL144基因具有较高的同源性,经过序列的比对,发现UL144基因DNA序列比较保守,只在4处有变异,且变异均为碱基替换,无插入或缺失,编码蛋白由176个氨基酸残基组成,氨基酸序列也比较保守,各分离株中变异率为1.1%;HCMV UL144编码蛋白翻译后修饰位点在所有分离株中均高度保守;所有分离株UL144蛋白的等电点均为8.97.结论 广州地区临床低传代分离株HCMV UL144基因DNA及其编码产物的氨基酸序列是比较保守的,但仍存在一定的多态性.提示UL144基因在先天性感染中可能具有重要作用.%Objective To investigate the polymorphism of human cytomegalovirus (HCMV) UL144 gene of the low passage clinical isolates in Guangzhou and explore the role of UL144 gene in HCMV pathogenicity. Methods The clinical isolates of HCMV were obtained from the urine sample collected from those infants with intra-uterus HCMV infection in Guangzhou. The virus genome DNA was extracted. According to the genome sequence of Toledo, primers for UL144 gene were designed and used to amplify the complete open reading frames (ORF) of the UL144 gene in our 3 different clinical isolates. These ORFs of the UL144 gene were cloned into pMD18-T vector

  3. An Alternative to Language Learner Dependence on L2 Caption-Reading Input for Comprehension of Sitcoms in a Multimedia Learning Environment

    Science.gov (United States)

    Li, C.-H.

    2014-01-01

    Most second/foreign language (L2) learners have difficulty understanding listening input because of its implicit and ephemeral nature, and they typically have better reading comprehension than listening comprehension skills. This study examines the effects of using an interactive advance-organizer activity on the DVD video comprehension of L2…

  4. Algebra of timed frames

    NARCIS (Netherlands)

    Bergstra, J.A.; Fokkink, W.J.; Middelburg, C.A.

    2008-01-01

    Timed frames are introduced as objects that can form a basis of a model theory for discrete time process algebra. An algebraic setting for timed frames is proposed and results concerning its connection with discrete time process algebra are given. The presented theory of timed frames captures the ba

  5. MAKING STUDENTS’ FRAMES EXPLICIT

    DEFF Research Database (Denmark)

    Nielsen, Louise Møller; Hansen, Poul Henrik Kyvsgaard

    2016-01-01

    Framing is a vital part of the design and innovation process. Frames are cognitive shortcuts (i.e. metaphors) that enable designers to connect insights about i.e. market opportunities and users needs with a set of solution principles and to test if this connection makes sense. Until now, framing ...

  6. Dynamics of students’ epistemological framing in group problem solving

    Science.gov (United States)

    Nguyen, Hai D.; Chari, Deepa N.; Sayre, Eleanor C.

    2016-11-01

    Many studies have investigated students’ epistemological framing when solving physics problems. Framing supports students’ problem solving as they decide what knowledge to employ and the necessary steps to solve the problem. Students may frame the same problem differently and take alternative paths to a correct solution. When students work in group settings, they share and discuss their framing to decide how to proceed in problem solving as a whole group. In this study, we investigate how groups of students negotiate their framing and frame shifts in group problem solving.

  7. Dynamics of students' epistemological framing in group problem solving

    CERN Document Server

    Nguyen, Hai D; Sayre, Eleanor C

    2016-01-01

    Many studies have investigated students' epistemological framing when solving physics problems. Framing supports students' problem solving as they decide what knowledge to employ and the necessary steps to solve the problem. Students may frame the same problem differently and take alternate paths to a correct solution. When students work in group settings, they share and discuss their framing to decide how to proceed in problem solving as a whole group. In this study, we investigate how groups of students negotiate their framing and frame shifts in group problem solving.

  8. On the interaction between media frames and individual frames of the Israeli-Palestinian conflict

    Directory of Open Access Journals (Sweden)

    Wilhelm Kempf

    2012-10-01

    Full Text Available The present paper reports on a media effects research experiment in which six groups of participants were asked to read and evaluate differently framed news articles about two scenarios: a Palestinian attack on Israel and an Israeli military operation against Palestinians. The experimental results show that media peace frames of violent events in the Israeli-Palestinian conflict are generally regarded by the German public as more comprehensible, less biased, more balanced and less partisan than media war frames of the same events. The specific ways in which recipients respond to the frames, however, depend on their prior knowledge of the conflict, on their positioning to the conflict and on their sensitivity to the ambivalence of war and peace for both Israel and the Palestinians. This supports the hypothesis that neither news selection nor framing have uniform effects on public opinion.

  9. Multivariate wavelet frames

    CERN Document Server

    Skopina, Maria; Protasov, Vladimir

    2016-01-01

    This book presents a systematic study of multivariate wavelet frames with matrix dilation, in particular, orthogonal and bi-orthogonal bases, which are a special case of frames. Further, it provides algorithmic methods for the construction of dual and tight wavelet frames with a desirable approximation order, namely compactly supported wavelet frames, which are commonly required by engineers. It particularly focuses on methods of constructing them. Wavelet bases and frames are actively used in numerous applications such as audio and graphic signal processing, compression and transmission of information. They are especially useful in image recovery from incomplete observed data due to the redundancy of frame systems. The construction of multivariate wavelet frames, especially bases, with desirable properties remains a challenging problem as although a general scheme of construction is well known, its practical implementation in the multidimensional setting is difficult. Another important feature of wavelet is ...

  10. IRREGULAR WAVELET FRAMES AND GABOR FRAMES

    Institute of Scientific and Technical Information of China (English)

    Ole Christensen; Sergio Favier; Felipe Zó

    2001-01-01

    Given g ∈ L2 (R), we consider irregular wavelet systems of theform {λ g(λj x-kb) }j z. kz, where λj >0 and b>0. Sufficient conditions for the wavelet system to constitute a frame for L2(R) are given. For a class of functions g ∈ L 2 (R) we prove that certain growth conditions on {λj} will lead to frames, and that some other types of sequences exclude the frame property. We also give a sufficient condition for a Gabor system {exib(j,x)g(x-λh)}jzn,kz to be a frame.CLC Number:O17 Document ID:AAuthor Resume:Ole Christensen;E-mail: Ole. Christensen@mat. dtu. dk Sergio Favier and Felipe Zó ;e-mails : sfavier@unsl. edu. ar fzo@unsl. edu. ar References:[1]Casaza,P.G. and Christensen,O.,Weyl-Heisenberg Frames for Subspaces of L2(R),Proc.Amer. Math. Soc. ,129(2001),145-154.[2]Casazza,P.G. and Christensen,O. ,Classifying Certain Irregular Gabor Frames,preprint[2]001.[3]Christensen,O. and Lindner,A. ,Lower Bounds for Finite Wavelet and Gabor Systems,Appr. Theory and Appl.,17(2001),17-31.[4]Chui,C.K. and Shi,X. ,Inequalities of Litdewood-Paley Type for Frames and Wavelets,SIAM J. Math. Anal. ,24:1(1993),263-277.[5]Daubechies,I. ,Ten Lectures on Wavelets,SIAM,Philadelphia,1992.[6]Favier,S. and Zalik,R. ,On the Stability of Frames and Riesz Bases,Appl. Comp. Ham.Anal. ,2(1995),160-173.[7]Feichtinger,H.G. and Strohmer,T. ,(Eds.),Gabor Analysis and Algorithms : Theory and Applications,Birkhauser,1998.[8]Heil,C.E. and Walnut,D.F. ,Continuous and Discrete Wavelet Transforms,SIAM Review,31:4(1989),628-666.[9]Sun,W. and Zhou,X. ,Irregular Wavelet Frames,Science in China (Series A),43:2(2000),122-127.Manuscript Received:2001年7月10日Manuscript Revised:2001年7月23日Published:2001年9月1日

  11. Soul of the Documentary. Framing, Expression, Ethics

    OpenAIRE

    Hongisto, Ilona

    2015-01-01

    ‘Soul of the Documentary’ offers a groundbreaking new approach to documentary cinema. Ilona Hongisto stirs current thinking by suggesting that the work of documentary films is not reducible to representing what already exists. By close-reading a diverse body of films - from ‘The Last Bolshevik’ to ‘Grey Gardens’ - Hongisto shows how documentary cinema intervenes in the real by framing it and creatively contributes to its perpetual unfolding. The emphasis on framing brings new urgency to the d...

  12. Frames and fusion frames in quantum optics

    Energy Technology Data Exchange (ETDEWEB)

    Jamiolkowski, Andrzej, E-mail: jam@fizyka.umk.p [Institute of Physics, Nicholas Copernicus University, 87-100 Torun (Poland)

    2010-03-01

    The main purpose of this paper is to show that the notions of frames and fusion frames introduced in non-harmonic Fourier analysis are also very natural in discussion of some basic problems in theory of open quantum systems and, in particular, in quantum optics. Frames are collections of vectors in a Hilbert space which assure a natural representation of each vector in the space, but may have infinitely many different representations for any given vector. For a given quantum system represented in a Hilbert space H the question of minimal number {eta} of observables Q{sub 1},..., Q{sub {eta}} whose expectation values at some instants t{sub 1},...,t{sub p} determine the statistical state of the system is discussed. We assume that the time evolution of the system in question is governed by a semigroup of linear transformations with generator L.

  13. Frame independent cosmological perturbations

    Energy Technology Data Exchange (ETDEWEB)

    Prokopec, Tomislav; Weenink, Jan, E-mail: t.prokopec@uu.nl, E-mail: j.g.weenink@uu.nl [Institute for Theoretical Physics and Spinoza Institute, Utrecht University, Leuvenlaan 4, 3585 CE Utrecht (Netherlands)

    2013-09-01

    We compute the third order gauge invariant action for scalar-graviton interactions in the Jordan frame. We demonstrate that the gauge invariant action for scalar and tensor perturbations on one physical hypersurface only differs from that on another physical hypersurface via terms proportional to the equation of motion and boundary terms, such that the evolution of non-Gaussianity may be called unique. Moreover, we demonstrate that the gauge invariant curvature perturbation and graviton on uniform field hypersurfaces in the Jordan frame are equal to their counterparts in the Einstein frame. These frame independent perturbations are therefore particularly useful in relating results in different frames at the perturbative level. On the other hand, the field perturbation and graviton on uniform curvature hypersurfaces in the Jordan and Einstein frame are non-linearly related, as are their corresponding actions and n-point functions.

  14. Reading Comics

    Science.gov (United States)

    Tilley, Carol L.

    2008-01-01

    Many adults, even librarians who willingly add comics to their collections, often dismiss the importance of comics. Compared to reading "real" books, reading comics appears to be a simple task and compared to reading no books, reading comics might be preferable. After all, comics do have words, but the plentiful pictures seem to carry most of the…

  15. Discretization of Continuous Frame

    Indian Academy of Sciences (India)

    A Fattahi; H Javanshiri

    2012-05-01

    In this paper we consider the notion of continuous frame of subspaces and define a new concept of continuous frame, entitled continuous atomic resolution of identity, for arbitrary Hilbert space $\\mathcal{H}$ which has a countable reconstruction formula. Among the other results, we characterize the relationship between this new concept and other known continuous frames. Finally, we state and prove the assertions of the stability of perturbation in this concept.

  16. Irregular wavelet frames

    Institute of Scientific and Technical Information of China (English)

    孙文昌; 周性伟

    2000-01-01

    For the non-band-limited function ψ, a sufficient condition is presented under whichis a frame for L2(R). The stability of these frames is studied. For the wavelets frequently used in signal processing, some concrete results are given.

  17. Irregular wavelet frames

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    For the non-band-limited function ψ, a sufficient condition is presented under which{√sjψ(sj·-kb)} is a frame for L2(R). The stability of these frames is studied. For the wavelets frequently used in signal processing, some concrete results are given.

  18. The Frame Game

    Science.gov (United States)

    Edwards, Michael Todd; Cox, Dana C.

    2011-01-01

    In this article, the authors explore framing, a non-multiplicative technique commonly employed by students as they construct similar shapes. When students frame, they add (or subtract) a "border" of fixed width about a geometric object. Although the approach does not yield similar shapes in general, the mathematical underpinnings of…

  19. Modern Steel Framed Schools.

    Science.gov (United States)

    American Inst. of Steel Construction, Inc., New York, NY.

    In view of the cost of structural framing for school buildings, ten steel-framed schools are examined to review the economical advantages of steel for school construction. These schools do not resemble each other in size, shape, arrangement or unit cost; some are original in concept and architecture, and others are conservative. Cost and…

  20. Back-illuminated large area frame transfer CCDs for space-based hyper-spectral imaging applications

    Science.gov (United States)

    Philbrick, Robert H.; Gilmore, Angelo S.; Schrein, Ronald J.

    2016-07-01

    Standard offerings of large area, back-illuminated full frame CCD sensors are available from multiple suppliers and they continue to be commonly deployed in ground- and space-based applications. By comparison the availability of large area frame transfers CCDs is sparse, with the accompanying 2x increase in die area no doubt being a contributing factor. Modern back-illuminated CCDs yield very high quantum efficiency in the 290 to 400 nm band, a wavelength region of great interest in space-based instruments studying atmospheric phenomenon. In fast framing (e.g. 10 - 20 Hz), space-based applications such as hyper-spectral imaging, the use of a mechanical shutter to block incident photons during readout can prove costly and lower instrument reliability. The emergence of large area, all-digital visible CMOS sensors, with integrate while read functionality, are an alternative solution to CCDs; but, even after factoring in reduced complexity and cost of support electronics, the present cost to implement such novel sensors is prohibitive to cost constrained missions. Hence, there continues to be a niche set of applications where large area, back-illuminated frame transfer CCDs with high UV quantum efficiency, high frame rate, high full well, and low noise provide an advantageous solution. To address this need a family of large area frame transfer CCDs has been developed that includes 2048 (columns) x 256 (rows) (FT4), 2048 x 512 (FT5), and 2048 x 1024 (FT6) full frame transfer CCDs; and a 2048 x 1024 (FT7) split-frame transfer CCD. Each wafer contains 4 FT4, 2 FT5, 2 FT6, and 2 FT7 die. The designs have undergone radiation and accelerated life qualification and the electro-optical performance of these CCDs over the wavelength range of 290 to 900 nm is discussed.

  1. Framing effects over time: comparing affective and cognitive news frames

    NARCIS (Netherlands)

    Lecheler, S.; Matthes, J.

    2012-01-01

    A growing number of scholars examine the duration of framing effects. However, duration is likely to differ from frame to frame, depending on how strong a frame is. This strength is likely to be enhanced by adding emotional components to a frame. By means of an experimental survey design (n = 111),

  2. Multicultural Reading

    Science.gov (United States)

    Veltze, Linda

    2004-01-01

    Multicultural reading advocates believe in the power of literature to transform and to change people's lives. They take seriously the arguments that racism and prejudice can be lessened through multicultural reading, and also that children from undervalued societal groups who read books that depict people like themselves in a positive light will…

  3. FrameLink软件

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    Blackmagic Design在NAB2005新推出的FrameLink软件,将在DeckLink系列视频卡中免费提供。它支持DPX文件格式,兼容电影工作中的数字中间片(DI)环节。FrameLink允许在保持Quick-Time和AVI文件的速度和灵活性的同时兼容DPX文件。

  4. Two Combinations of Unitary Operators and Frame Representations

    Institute of Scientific and Technical Information of China (English)

    李祚; 朱红鲜; 张慧; 杜鸿科

    2005-01-01

    In this paper, we prove that the norm closure of all linear combinations of two unitary operators is equal to the norm closure of all invertible operators in B(H). We apply the results to frame representations and give some simple and alternative proofs of the propositions in “P. G. Casazza, Every frame is a sum of three (but not two) orthonormal bases-and other frame representations, J. Fourier Anal. Appl., 4(6)(1998), 727-732.”

  5. Language use depending on news frame and immigrant origin.

    Science.gov (United States)

    Fernández, Itziar; Igartua, Juan-José; Moral, Félix; Palacios, Elena; Acosta, Tania; Muñoz, Dolores

    2013-01-01

    The purpose of this study was to analyze the effect of the media on individuals' specific language use in relation to a news story on immigration: the influence of the news frame and group cue. Abstraction, complexity of language use, and negative affective language were evaluated. The 523 participants were randomly distributed to each of the four experimental conditions: news frame (crime versus economic contribution) by group cue (geographical origin of the immigrants involved: Moroccans versus Latin Americans). Through content analysis of the ideas and reflections that arose after the participants read the different news stories, using the Linguistic Category Model (LCM; Semin & Fiedler, 1991) to measure abstract language and the Linguistic Inquiry and Word Count (LIWC; Pennebaker, Booth, & Francis, 2007) to analyze complex language and negative affective language, it emerged that abstract language and negative affective language were more frequent in the participants assigned to the news frame on crime. Complex language was more commonly used when the news frame referred to the economic contribution of immigrants. Regression analyses showed the mediating role of attitude to immigration in the effects of news frame on negative affective language. The bootstrap method was used to assess the magnitude of the indirect effect. A significant mediator effect was also found through structural equation modeling. Analyses of covariance showed one interaction between news frame and group cue: Among those who read the news story in a frame linking immigration to crime and Moroccan origin, abstract language was more characteristic. The results are discussed from the theoretical perspective of framing.

  6. Type of frame dependence: variation in media frames affecting attitudes via audience frames

    NARCIS (Netherlands)

    van Drunen, A.S.; Boomgaarden, H.G.; Vliegenthart, R.

    2012-01-01

    In this paper two suggested processes of framing theory (Scheufele, 1999) are tested: frame setting (media affecting audience frames) and the individual effects process (audience frames affecting attitudes or behavior). Media frames may affect opinions and behavior, however these effects are not dir

  7. All Frames Are Not Created Equal: A Typology and Critical Analysis of Framing Effects.

    Science.gov (United States)

    Levin; Schneider; Gaeth

    1998-11-01

    Accentuate the positive or accentuate the negative? The literature has been mixed as to how the alternative framing of information in positive or negative terms affects judgments and decisions. We argue that this is because different studies have employed different operational definitions of framing and thus have tapped different underlying processes. We develop a typology to distinguish between three different kinds of valence framing effects. First we discuss the standard risky choice framing effect introduced by Tversky and Kahneman (1981) to illustrate how valence affects willingness to take a risk. Then we discuss attribute framing, which affects the evaluation of object or event characteristics, and goal framing, which affects the persuasiveness of a communication. We describe the distinctions, provide a number of examples of each type, and discuss likely theoretical mechanisms underlying each type of framing effect. Our typology helps explain and resolve apparent confusions in the literature, ties together studies with common underlying mechanisms, and serves as a guide to future research and theory development. We conclude that a broader perspective, focused on the cognitive and motivational consequences of valence-based encoding, opens the door to a deeper understanding of the causes and consequences of framing effects. Copyright 1998 Academic Press.

  8. A Case for Slow Reading

    Directory of Open Access Journals (Sweden)

    Matthew J Ostercamp

    2014-05-01

    Full Text Available This essay makes a case for the value of slow or deep reading.  Inspired by the Slow Food movement it seeks to apply their principles to reading.  It begins by exploring the meaning of information and how like food, information has come to be regarded as a commodity.  Drawing upon the philosophy of Albert Borgmann, it counters the prevalent commodity view of information by offering an alternative paradigm that connects careful reading to human flourishing.  It argues that by connecting information to pleasure and community, slow reading advocates can have comparable success to that enjoyed by the slow food movement.

  9. Line defects and (framed) BPS quivers

    CERN Document Server

    Cirafici, Michele

    2013-01-01

    The BPS spectrum of certain N=2 supersymmetric field theories can be determined algebraically by studying the representation theory of BPS quivers. We introduce methods based on BPS quivers to study line defects. The presence of a line defect opens up a new BPS sector: framed BPS states can be bound to the defect. The defect can be geometrically described in terms of laminations on a curve. To a lamination we associate certain elements of the Leavitt path algebra of the BPS quiver and use them to compute the framed BPS spectrum. We also provide an alternative characterization of line defects by introducing framed BPS quivers. Using the theory of (quantum) cluster algebras, we derive an algorithm to compute the framed BPS spectra of new defects from known ones. Line defects are generated from a framed BPS quiver by applying certain sequences of mutation operations. Framed BPS quivers also behave nicely under a set of "cut and join" rules, which can be used to study how N=2 systems with defects couple to produc...

  10. Constitutionalizing secularism, alternative secularisms or liberal-democratic constitutionalism?A critical reading of some Turkish, ECtHR and Indian Supreme Court cases on ‘secularism’

    Directory of Open Access Journals (Sweden)

    Veit Bader

    2010-11-01

    Full Text Available In recent debates on the constitutional status of 'secularism' we can discern three positions. The first tries to overcome the absence of 'secularism' in most liberal-democratic constitutions by developing a more robust theory of constitutional secularism. The second develops theories of 'alternative secularisms'. The third, defended in this article, argues that we should drop secularism as a 'cacophonous' concept from our constitutional and legal language and replace it by liberal-democratic constitutionalism. I develop an analytical taxonomy of twelve different meanings of 'secularism' based on a comparative study of Turkish and Indian Supreme Court cases on secularism, and demonstrate that they are incompatible with each other and with the hard core of liberal-democratic constitutions. Next, I criticize the respective rulings in the Turkish and Indian context. Particularly in 'militant democracies', the appeal to a principle of 'secularism' turns out to be inimical to the liberal and to the democratic 'constitutional essentials'. I end with some normative recommendations on the role of constitutional review and judicial activism.

  11. New Characterizations of Fusion Frames (Frames of Subspaces)

    Indian Academy of Sciences (India)

    Mohammad Sadegh Asgari

    2009-06-01

    In this article, we give new characterizations of fusion frames, on the properties of their synthesis operators, on the behavior of fusion frames under bounded operators with closed range, and on erasures of subspaces of fusion frames. Furthermore we show that every fusion frame is the image of an orthonormal fusion basis under a bounded surjective operator.

  12. Framing financial culture

    DEFF Research Database (Denmark)

    Just, Sine Nørholm; Mouton, Nicolaas T.O.

    2014-01-01

    /methodology/approach – A conceptual framework that combines modern framing theory, and classical stasis theory is applied to the rhetorical struggles over the meaning of “Liborgate.” Findings – While rhetorical struggles over “Liborgate” overtly center on the issue of who is to blame, an analysis of the argumentative relations...... between competing frames leads to the conclusion that this political “blame game” is related to struggles over how to define the scandal, how to conceptualize its causes, and policy recommendations. Banks may have lost the battle of “Liborgate,” but the war over the meaning of financial culture is far...... from over. Originality/value – The paper is theoretically and methodologically original in its combination of the theories of framing and stasis, and it provides analytical insights into how sense is made of financial culture in the wake of the financial crisis....

  13. Conformal frames in cosmology

    CERN Document Server

    Domènech, Guillem

    2016-01-01

    From higher dimensional theories, e.g. string theory, one expects the presence of non-minimally coupled scalar fields. We review the notion of conformal frames in cosmology and emphasize their physical equivalence, which holds at least at a classical level. Furthermore, if there is a field, or fields, which dominates the universe, as it is often the case in cosmology, we can use such notion of frames to treat our system, matter and gravity, as two different sectors. On one hand, the gravity sector which describes the dynamics of the geometry and on the other hand the matter sector which has such geometry as a playground. We use this interpretation to build a model where the fact that a curvaton couples to a particular frame metric could leave an imprint in the CMB.

  14. Framing (implicitly) matters

    DEFF Research Database (Denmark)

    Anderson, Joel; Antalikova, Radka

    2014-01-01

    Denmark is currently experiencing the highest immigration rate in its modern history. Population surveys indicate that negative public attitudes toward immigrants actually stem from attitudes toward their (perceived) Islamic affiliation. We used a framing paradigm to investigate the explicit...... and implicit attitudes of Christian and Atheist Danes toward targets framed as Muslims or as immigrants. The results showed that explicit and implicit attitudes were more negative when the target was framed as a Muslim, rather than as an immigrant. Interestingly, implicit attitudes were qualified...... by the participants’ religion. Specifically, analyses revealed that Christians demonstrated more negative implicit attitudes toward immigrants than Muslims. Conversely, Atheists demonstrated more negative implicit attitudes toward Muslims than Atheists. These results suggest a complex relationship between religion...

  15. FishFrame

    DEFF Research Database (Denmark)

    Degel, Henrik; Jansen, Teunis

    2006-01-01

    . Development and test of software modules can be done once and reused by all. The biggest challenge in this is not technical – it is in organisation, coordination and trust. This challenge has been addressed by FishFrame - a web-based datawarehouse application. The “bottom-up” approach with maximum involvement...... value to users and in the end improves the way we work with our data. FishFrame version 4.2 is presented and the lessons learned from the process are discussed....

  16. Framings for graph hypersurfaces

    CERN Document Server

    Brown, Francis

    2013-01-01

    We present a method for computing the framing on the cohomology of graph hypersurfaces defined by the Feynman differential form. This answers a question of Bloch, Esnault and Kreimer in the affirmative for an infinite class of graphs for which the framings are Tate motives. Applying this method to the modular graphs of Brown and Schnetz, we find that the Feynman differential form is not of Tate type in general. This finally disproves a folklore conjecture stating that the periods of Feynman integrals of primitive graphs in phi^4 theory factorise through a category of mixed Tate motives.

  17. Frames and extension problems I

    DEFF Research Database (Denmark)

    Christensen, Ole

    2014-01-01

    In this article we present a short survey of frame theory in Hilbert spaces. We discuss Gabor frames and wavelet frames and set the stage for a discussion of various extension principles; this will be presented in the article Frames and extension problems II (joint with H.O. Kim and R.Y. Kim)....

  18. A putative helicase, the SUA5, PMR1, tRNALys1 genes and four open reading frames have been detected in the DNA sequence of an 8.8 kb fragment of the left arm of chromosome VII of Saccharomyces cerevisiae.

    Science.gov (United States)

    Klima, R; Coglievina, M; Zaccaria, P; Bertani, I; Bruschi, C V

    1996-09-01

    We report the sequence of an 8.8 kb segment of DNA from the left arm of chromosome VII of Saccharomyces cerevisiae. The sequence reveals seven open reading frames (ORFs) G1651, G1654, G1660, G1663, G1666, G1667 and G1669 greater than 100 amino acids in length and the tRNALys1 gene. ORF G1651 shows 100% identity with the ROK1 protein which is a putative RNA helicase of the 'DEAD box' protein family. ORF G1654 exhibits a motif highly conserved in ATP/GTP binding proteins generally referred to as 'P-loop'. From FastA analysis, G1660 and G1666 were found to be previously sequenced genes, respectively SUA5 and PMR1. The three other ORFs identified are partially (G1663) or completely (G1667 and G1669) overlapping with the PMR1 sequence on the complementary strand. This feature, together with their low codon adaptation indexes and the absence of significant homology with known proteins suggest that they do not correspond to real genes.

  19. Framing difficulties in quantum mechanics

    CERN Document Server

    Modir, Bahar; Sayre, Eleanor C

    2016-01-01

    Students' difficulties in quantum mechanics may be the result of unproductive framing and not fundamental inability to solve the problems or misconceptions about physics content. We observed groups of students solving quantum mechanics problems in an upper-division physics course. Using the lens of the epistemological framing, we investigated four frames in our observational data: algorithmic math, conceptual math, algorithmic physics, and conceptual physics. We then used our framework to seek an underlying structure to the long lists of published difficulties that span many topics in quantum mechanics. We mapped descriptions of published difficulties into errors in epistemological framing and resource use. We analyzed descriptions of students' problem solving to find their frames, and compared students' framing to framing (and frame shifting) required by problem statements. We found three categories of error: mismatches between students' framing and problem statement framing; inappropriate or absent transiti...

  20. Framing Evolution Discussion Intellectually

    Science.gov (United States)

    Oliveira, Alandeom W.; Cook, Kristin; Buck, Gayle A.

    2011-01-01

    This study examines how a first-year biology teacher facilitates a series of whole-class discussions about evolution during the implementation of a problem-based unit. A communicative theoretical perspective is adopted wherein evolution discussions are viewed as social events that the teacher can frame intellectually (i.e., present or organize as…

  1. Institutional Justification in Frames

    DEFF Research Database (Denmark)

    Baden, Christian; Schultz, Friederike

    Crises – such as the BP oil spill in the Gulf of Mexico (2010) – are moments in which conventional interpretations break down. This paper analyzes the dynamics of the re-construction and stabilization of meaning in the public debate and assesses how BP’s strategic framing affected the emerging...

  2. What's in a Frame?

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    Maintaining a good image and reputation in the eyes of stakeholders is vital to the organisation. Thus, in its corporate communication and discourse the organisation will seek to present or frame itself as favourably as possible while observing regulations stipulating accuracy and precision in th....../NY: Continuum. Hallahan, Kirk. 1999. Seven models of framing: Implications for public relations. Journal of Public Relations Research, 11(3): 205-242....... in the information given. However, in this process the organisation is not operating in a social vacuum but is continuously interacting with various players, such as the media, for its textual and discursive creation (Gunnarsson 2009). In this presentation, I will focus on the role of this interaction in framing...... considerable strain on the Bank and its image, leading it to reconsider its previous strategy of denial of responsibility. References Entman, Robert M. 1993. Framing: Toward clarification of a fractured paradigm. Journal of Communication, 41: 6-25. Gunnarsson, Britt-Louise. 2009. Professional Discourse. London...

  3. Framing ‘fracking’

    NARCIS (Netherlands)

    Williams, Laurence; Macnaghten, Philip; Davies, Richard; Curtis, Sarah

    2017-01-01

    The prospect of fracking in the United Kingdom has been accompanied by significant public unease. We outline how the policy debate is being framed by UK institutional actors, finding evidence of a dominant discourse in which the policy approach is defined through a deficit model of public understand

  4. The Cage; Framing Dad

    DEFF Research Database (Denmark)

    Kau, Edvin

    2012-01-01

    Unfolding his story very gradually and arousing the viewer’s curiosity, Sitaru invites the audience to investigate the parents’ and the boy’s mutual positions in their small flat, as well as the various layers of their conversations, through such means as framing, editing style, and the use...

  5. How Reading Volume Affects both Reading Fluency and Reading Achievement

    Directory of Open Access Journals (Sweden)

    Richard L. ALLINGTON

    2014-10-01

    Full Text Available Long overlooked, reading volume is actually central to the development of reading proficiencies, especially in the development of fluent reading proficiency. Generally no one in schools monitors the actual volume of reading that children engage in. We know that the commonly used commercial core reading programs provide only material that requires about 15 minutes of reading activity daily. The remaining 75 minute of reading lessons is filled with many other activities such as completing workbook pages or responding to low-level literal questions about what has been read. Studies designed to enhance the volume of reading that children do during their reading lessons demonstrate one way to enhance reading development. Repeated readings have been widely used in fostering reading fluency but wide reading options seem to work faster and more broadly in developing reading proficiencies, including oral reading fluency.

  6. Promoting preschool reading

    OpenAIRE

    2013-01-01

    The thesis titled Promoting preschool reading consists of a theoretiral and an empirical part. In the theoretical part I wrote about reading, the importance of reading, types of reading, about reading motivation, promoting reading motivation, internal and external motivation, influence of reading motivation on the child's reading activity, reading and familial literacy, the role of adults in promotion reading literacy, reading to a child and promoting reading in pre-school years, where I ...

  7. Reading Evaluation

    Science.gov (United States)

    Fagan, W. T.

    1978-01-01

    The Canadian Institute for Research in Behavioral and Social Sciences of Calgary was awarded a contract by the Provincial Government of Alberta to assess student skills and knowledge in reading and written composition. Here evaluation is defined and the use of standardized and criterion referenced tests for evaluating reading performance are…

  8. Teaching Reading.

    Science.gov (United States)

    Ricketts, Mary

    1980-01-01

    Described are five approaches to teaching reading: Language Experience, Modified Alphabet, Linguistic, Programmed, and Basal. It is suggested that a good teacher, well trained, certified in his or her profession, an active participant in professional organizations, can teach reading successfully using almost any approach. (KC)

  9. Reading Letters

    DEFF Research Database (Denmark)

    Beier, Sofie

    2012-01-01

    In our everyday life we constantly encounter a diversity of reading matters, including display types on traffic signage, printed text in novels, newspaper headlines, or our own writing on a computer screen. All these conditions place different demands on the typefaces applied. The book discusses ...... these aspects by drawing on typography history, designers’ ideas, and available scientific data concerning the reading process....

  10. ON THE STABILITY OF FUSION FRAMES (FRAMES OF SUBSPACES)

    Institute of Scientific and Technical Information of China (English)

    Mohammad Sadegh Asgari

    2011-01-01

    A frame is an orthonormal basis-like collection of vectors in a Hilbert space,but need not be a basis or orthonormal.A fusion frame (frame of subspaces) is a frame-like collection of subspaces in a Hilbert space,thereby constructing a frame for the whole space by joining sequences of frames for subspaces.Moreover the notion of fusion frames provide a framework for applications and providing efficient and robust information processing algorithms.In this paper we study the conditions under which removing an element from a fusion frame,again we obtain another fusion frame.We give another proof of[5,Corollary 3.3(iii)]with extra information about the bounds.

  11. Some relationship between G-frames and frames

    Directory of Open Access Journals (Sweden)

    Mehdi Rashidi-Kouchi

    2015-06-01

    Full Text Available In this paper we proved that every g-Riesz basis for Hilbert space $H$ with respect to $K$ by adding a condition is a Riesz basis for Hilbert $B(K$-module $B(H,K$. This is an extension of [A. Askarizadeh,M. A. Dehghan, {em G-frames as special frames}, Turk. J. Math., 35, (2011 1-11]. Also, we derived similar results for g-orthonormal and orthogonal bases. Some relationships between dual frame, dual g-frame and exact frame and exact g-frame are presented too.

  12. Book Reading in Leisure Time: Long-Term Changes in Young Peoples' Book Reading Habits

    Science.gov (United States)

    Johnsson-Smaragdi, Ulla; Jonsson, Annelis

    2006-01-01

    Visual and ICT media are often perceived as a threat to book reading in leisure time. They are accused of taking time and interest away from children and adolescents' book reading by offering them more approachable alternatives. Children and adolescents' book reading habits and the way these habits have changed over time are in focus. Is there any…

  13. A Comparison of Two Reading Fluency Methods: Repeated Readings to a Fluency Criterion and Interval Sprinting

    Science.gov (United States)

    Kostewicz, Douglas E.; Kubina, Richard M., Jr.

    2010-01-01

    Teachers have used the method of repeated readings to build oral reading fluency in students with and without special needs. A new fluency building intervention called interval sprinting uses shorter timing intervals (i.e., sprints) across a passage. This study used an alternating treatment design to compare repeated readings and interval…

  14. Improving Oral Reading Fluency through Response Opportunities: A Comparison of Phrase Drill Error Correction with Repeated Readings

    Science.gov (United States)

    Begeny, John C.; Daly, Edward J., III; Valleley, Rachel J.

    2006-01-01

    The purpose of this study was to compare two oral reading fluency treatments (repeated readings and phrase drill error correction) which differ in the way they prompt student responding. Repeated readings (RR) and phrase drill (PD) error correction were alternated with a baseline and a reward condition within an alternating treatments design with…

  15. Framing Light Rail Projects

    DEFF Research Database (Denmark)

    Olesen, Mette

    2014-01-01

    In Europe, there has been a strong political will to implement light rail. This article contributes to the knowledge concerning policies around light rail by analysing how local actors frame light rail projects and which rationalities and arguments are present in this decision-making process....... The article draws on the socio-technical approach to mobilities studies in order to reassemble the decision-making process in three European cases: Bergen, Angers, and Bern. This article provides insights into the political, discursive and material production of light rail mobilities in a European context....... It identifies the planning rationales behind the systems and the policies that have been supportive of this light rail vision. Finally, the article identifies the practical challenges and potentials that have been connected to the different local frames of light rail mobility which can be used in future...

  16. Rigid Quasilocal Frames

    CERN Document Server

    McGrath, Paul L

    2014-01-01

    In this thesis, I examine in detail the properties of rigid quasilocal frames (RQF), which have been proposed as a geometrically natural way to define spatially extended reference frames in general relativity. I also explore their usefulness, in particular, as a tool for constructing completely general conservation laws that do not rely on the presence of spacetime symmetries and include both matter and gravitational contributions without the need for any ad hoc structures such as pseudotensors. In doing so, I show how the RQF approach affords a deeper understanding of the nature of gravitational fluxes via the equivalence principle. Finally, I apply the RQF formalism to explore Ehrenfest's rotating disk paradox, a generalization of Archimedes' law to curved spacetime, tidal interactions for Earth's and Jupiter's moons, and more.

  17. Framing the Manager

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    2013-01-01

    Genres are ways for organisations of discursively interacting with the surrounding world, with the aim of achieving specific disciplinary goals (Bhatia 2004). As such, the management job ad has the objective of finding the right candidate for the management job advertised (Norlyk 2006...... predominant conceptual models or frames for leadership, the ‘goal-oriented motivator’ model and the ‘empathic communicator’ model, which frame the position and the candidate, from the discursive and metaphorical construction of the ad to the actual hiring of the new employee. While there may be little new...... in this realisation, the fact that one of the two models, the ‘goal-oriented motivator’ model, seems to be monopolising the genre raises a number of issues that need to be addressed: How is this model realised conceptually and linguistically? Why does this model continue to be the Danish business world’s preferred...

  18. Electronic Photo Frame

    OpenAIRE

    Ni, Ze

    2010-01-01

    The goal of the thesis work is to develop a photo frame device. Hence, it supplies a set of feasible solutions from hardware to software. Relatively speaking, the project chiefly focuses on software-oriented development. It is concerned with building the bootloader, the Linux Kernel, related drivers, file systems, and applications. After that, they are integrated together to form a brand-new embedded Linux distribution. The distribution is specifically designed to meet the requirement of the ...

  19. Density of Gabor Frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Heil, C.; Deng, B.

    1997-01-01

    A Gabor system is a set of time-frequency shifts$S(g,\\Lambda) = \\{e^{2\\pi i b x} g(x-a)\\}_{(a,b) \\in \\Lambda}$of a function $g \\in L^2({\\bold R}^d)$.We prove that if a finite union of Gabor systems$\\bigcup_{k=1}^r S(g_k,\\Lambda_k)$, with arbitrary sequences $\\Lambda_k$,forms a frame for $L^2({\\bo...

  20. Translational regulation of human neuronal nitric-oxide synthase by an alternatively spliced 5'-untranslated region leader exon.

    Science.gov (United States)

    Newton, Derek C; Bevan, Sian C; Choi, Stephen; Robb, G Brett; Millar, Adam; Wang, Yang; Marsden, Philip A

    2003-01-03

    Expression of the neuronal nitric-oxide synthase (nNOS) mRNA is subject to complex cell-specific transcriptional regulation, which is mediated by alternative promoters. Unexpectedly, we identified a 89-nucleotide alternatively spliced exon located in the 5'-untranslated region between exon 1 variants and a common exon 2 that contains the translational initiation codon. Alternative splicing events that do not affect the open reading frame are distinctly uncommon in mammals; therefore, we assessed its functional relevance. Transient transfection of reporter RNAs performed in a variety of cell types revealed that this alternatively spliced exon acts as a potent translational repressor. Stably transfected cell lines confirmed that the alternatively spliced exon inhibited translation of the native nNOS open reading frame. Reverse transcription-PCR and RNase protection assays indicated that nNOS mRNAs containing this exon are common and expressed in both a promoter-specific and tissue-restricted fashion. Mutational analysis identified the functional cis-element within this novel exon, and a secondary structure prediction revealed that it forms a putative stem-loop. RNA electrophoretic mobility shift assay techniques revealed that a specific cytoplasmic RNA-binding complex interacts with this motif. Hence, a unique splicing event within a 5'-untranslated region is demonstrated to introduce a translational control element. This represents a newer model for the translational control of a mammalian mRNA.

  1. Framing the Text: Using Storyboards to Engage Students with Reading

    Science.gov (United States)

    Bruce, David L.

    2011-01-01

    Storyboards deliver a narrative through discrete visual representations. The purpose of the storyboards was always to "scaffold" the final product and students were free to add, delete, or adapt those images that were most helpful to their project. The storyboards served as a brainstorming activity, much like a prewriting exercise for a written…

  2. Effects of news frames on perceived risk, emotions, and learning.

    Directory of Open Access Journals (Sweden)

    Christine Otieno

    Full Text Available The media play a key role in forming opinions by influencing people´s understanding and perception of a topic. People gather information about topics of interest from the internet and print media, which employ various news frames to attract attention. One example of a common news frame is the human-interest frame, which emotionalizes and dramatizes information and often accentuates individual affectedness. Our study investigated effects of human-interest frames compared to a neutral-text condition with respect to perceived risk, emotions, and knowledge acquisition, and tested whether these effects can be "generalized" to common variants of the human-interest frame. Ninety-one participants read either one variant of the human-interest frame or a neutrally formulated version of a newspaper article describing the effects of invasive species in general and the Asian ladybug (an invasive species in particular. The framing was achieved by varying the opening and concluding paragraphs (about invasive species, as well as the headline. The core text (about the Asian ladybug was the same across all conditions. All outcome variables on framing effects referred to this common core text. We found that all versions of the human-interest frame increased perceived risk and the strength of negative emotions compared to the neutral text. Furthermore, participants in the human-interest frame condition displayed better (quantitative learning outcomes but also biased knowledge, highlighting a potential dilemma: Human-interest frames may increase learning, but they also lead to a rather unbalanced view of the given topic on a "deeper level".

  3. Effects of News Frames on Perceived Risk, Emotions, and Learning

    Science.gov (United States)

    Otieno, Christine; Spada, Hans; Renkl, Alexander

    2013-01-01

    The media play a key role in forming opinions by influencing people´s understanding and perception of a topic. People gather information about topics of interest from the internet and print media, which employ various news frames to attract attention. One example of a common news frame is the human-interest frame, which emotionalizes and dramatizes information and often accentuates individual affectedness. Our study investigated effects of human-interest frames compared to a neutral-text condition with respect to perceived risk, emotions, and knowledge acquisition, and tested whether these effects can be "generalized" to common variants of the human-interest frame. Ninety-one participants read either one variant of the human-interest frame or a neutrally formulated version of a newspaper article describing the effects of invasive species in general and the Asian ladybug (an invasive species) in particular. The framing was achieved by varying the opening and concluding paragraphs (about invasive species), as well as the headline. The core text (about the Asian ladybug) was the same across all conditions. All outcome variables on framing effects referred to this common core text. We found that all versions of the human-interest frame increased perceived risk and the strength of negative emotions compared to the neutral text. Furthermore, participants in the human-interest frame condition displayed better (quantitative) learning outcomes but also biased knowledge, highlighting a potential dilemma: Human-interest frames may increase learning, but they also lead to a rather unbalanced view of the given topic on a “deeper level”. PMID:24223999

  4. MedlinePlus FAQ: Framing

    Science.gov (United States)

    ... URL of this page: https://medlineplus.gov/faq/framing.html I'd like to link to MedlinePlus, ... as HealthDay. Our license agreements do not permit framing of their content from our site. For more ...

  5. Frame by frame stop motion non-traditional approaches to stop motion animation

    CERN Document Server

    Gasek, Tom

    2011-01-01

    In a world that is dominated by computer images, alternative stop motion techniques like pixilation, time-lapse photography and down-shooting techniques combined with new technologies offer a new, tangible and exciting approach to animation. With over 25 years professional experience, industry veteran, Tom Gasek presents a comprehensive guide to stop motion animation without the focus on puppetry or model animation. With tips, tricks and hands-on exercises, Frame by Frame will help both experienced and novice filmmakers get the most effective results from this underutilized branch of animation

  6. New avenues for framing research

    NARCIS (Netherlands)

    de Vreese, C.H.

    2012-01-01

    In this article, the author reviews the studies in this special issue of the American Behavioral Scientist. It is a strong collection of articles reporting findings from an integrated project that looks at frame building, frames, and effects of frames. The project is part of an exciting large-scale

  7. The Levels of Visual Framing

    Science.gov (United States)

    Rodriguez, Lulu; Dimitrova, Daniela V.

    2011-01-01

    While framing research has centered mostly on the evaluations of media texts, visual news discourse has remained relatively unexamined. This study surveys the visual framing techniques and methods employed in previous studies and proposes a four-tiered model of identifying and analyzing visual frames: (1) visuals as denotative systems, (2) visuals…

  8. Synchronization and Inertial Frames

    CERN Document Server

    Viazminsky, C P

    1999-01-01

    In classical mechanics, a procedure for simultaneous synchronization in all inertial frames is consistent with the Galilean transformation. However, if one attempts to achieve such a synchronization utilizing light signals, he will be facing in the first place the break down of simultaneity, and secondly, a self-contradictory transformation that has the Lorentz transformation, or its confinement to the velocity of light, as the only possible ways that resolve the contradiction. The current work constitutes a smooth transition from traditional to relativistic vision of mechanics, and therefore is quite appealing from pedagogical point of view.

  9. Continuous Shearlet Tight Frames

    KAUST Repository

    Grohs, Philipp

    2010-10-22

    Based on the shearlet transform we present a general construction of continuous tight frames for L2(ℝ2) from any sufficiently smooth function with anisotropic moments. This includes for example compactly supported systems, piecewise polynomial systems, or both. From our earlier results in Grohs (Technical report, KAUST, 2009) it follows that these systems enjoy the same desirable approximation properties for directional data as the previous bandlimited and very specific constructions due to Kutyniok and Labate (Trans. Am. Math. Soc. 361:2719-2754, 2009). We also show that the representation formulas we derive are in a sense optimal for the shearlet transform. © 2010 Springer Science+Business Media, LLC.

  10. Framing a Bank

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    2012-01-01

    Maintaining a good image and reputation in the eyes of stakeholders is vital to the organisation. Thus, in its corporate communication and discourse the organisation will seek to present itself as favourably as possible while observing regulations stipulating accuracy and precision...... in the information given. However, in this process the organisation is not operating in a social vacuum but is continuously interacting with various players, such as the media, for its discursive construction. This article will focus on the role of this interaction in metaphorically and conceptually framing a large...

  11. VIOLENT FRAMES IN ACTION

    Energy Technology Data Exchange (ETDEWEB)

    Sanfilippo, Antonio P.; McGrath, Liam R.; Whitney, Paul D.

    2011-11-17

    We present a computational approach to radical rhetoric that leverages the co-expression of rhetoric and action features in discourse to identify violent intent. The approach combines text mining and machine learning techniques with insights from Frame Analysis and theories that explain the emergence of violence in terms of moral disengagement, the violation of sacred values and social isolation in order to build computational models that identify messages from terrorist sources and estimate their proximity to an attack. We discuss a specific application of this approach to a body of documents from and about radical and terrorist groups in the Middle East and present the results achieved.

  12. How Reading Volume Affects Both Reading Fluency and Reading Achievement

    Science.gov (United States)

    Allington, Richard L.

    2014-01-01

    Long overlooked, reading volume is actually central to the development of reading proficiencies, especially in the development of fluent reading proficiency. Generally no one in schools monitors the actual volume of reading that children engage in. We know that the commonly used commercial core reading programs provide only material that requires…

  13. Effects of publishing industry and reading culture

    OpenAIRE

    Maja Breznik

    2003-01-01

    The author presents some results form the research on publishing and librarianship, which was carried out in frame of the Peace Institute in Ljubljana from 2001 till 2003. The research focused on the reading cultures cultivated by Slovene publishers through their publishing programs and on the reading preferences in Slovene public libraries. The results of the research derive from samples of the Slovene annual publishing production in year 2000 and circulation numbers of these publications in...

  14. Student-generated reading questions: diagnosing student thinking with diverse formative assessments.

    Science.gov (United States)

    Offerdahl, Erika G; Montplaisir, Lisa

    2014-01-01

    Formative assessment has long been identified as a critical element to teaching for conceptual development in science. It is therefore important for university instructors to have an arsenal of formative assessment tools at their disposal which enable them to effectively uncover and diagnose all students' thinking, not just the most vocal or assertive. We illustrate the utility of one type of formative assessment prompt (reading question assignment) in producing high-quality evidence of student thinking (student-generated reading questions). Specifically, we characterized student assessment data using three distinct analytic frames to exemplify their effectiveness in diagnosing student learning in relationship to three sample learning outcomes. Our data will be useful for university faculty, particularly those engaged in teaching upper-level biochemistry courses and their prerequisites, as they provide an alternative mechanism for uncovering and diagnosing student understanding.

  15. Identifying issue frames in text.

    Directory of Open Access Journals (Sweden)

    Eyal Sagi

    Full Text Available Framing, the effect of context on cognitive processes, is a prominent topic of research in psychology and public opinion research. Research on framing has traditionally relied on controlled experiments and manually annotated document collections. In this paper we present a method that allows for quantifying the relative strengths of competing linguistic frames based on corpus analysis. This method requires little human intervention and can therefore be efficiently applied to large bodies of text. We demonstrate its effectiveness by tracking changes in the framing of terror over time and comparing the framing of abortion by Democrats and Republicans in the U.S.

  16. Cognitive framing in action.

    Science.gov (United States)

    Huhn, John M; Potts, Cory Adam; Rosenbaum, David A

    2016-06-01

    Cognitive framing effects have been widely reported in higher-level decision-making and have been ascribed to rules of thumb for quick thinking. No such demonstrations have been reported for physical action, as far as we know, but they would be expected if cognition for physical action is fundamentally similar to cognition for higher-level decision-making. To test for such effects, we asked participants to reach for a horizontally-oriented pipe to move it from one height to another while turning the pipe 180° to bring one end (the "business end") to a target on the left or right. From a physical perspective, participants could have always rotated the pipe in the same angular direction no matter which end was the business end; a given participant could have always turned the pipe clockwise or counter-clockwise. Instead, our participants turned the business end counter-clockwise for left targets and clockwise for right targets. Thus, the way the identical physical task was framed altered the way it was performed. This finding is consistent with the hypothesis that cognition for physical action is fundamentally similar to cognition for higher-level decision-making. A tantalizing possibility is that higher-level decision heuristics have roots in the control of physical action, a hypothesis that accords with embodied views of cognition.

  17. Semiclassical framed BPS states

    CERN Document Server

    Moore, Gregory W; Bleeken, Dieter Van den

    2015-01-01

    We provide a semiclassical description of framed BPS states in four-dimensional N = 2 super Yang-Mills theories probed by 't Hooft defects, in terms of a supersymmetric quantum mechanics on the moduli space of singular monopoles. Framed BPS states, like their ordinary counterparts in the theory without defects, are associated with the L^2 kernel of certain Dirac operators on moduli space, or equivalently with the L^2 cohomology of related Dolbeault operators. The Dirac/Dolbeault operators depend on two Cartan-valued Higgs vevs. We conjecture a map between these vevs and the Seiberg-Witten special coordinates, consistent with a one-loop analysis and checked in examples. The map incorporates all perturbative and nonperturbative corrections that are relevant for the semiclassical construction of BPS states, over a suitably defined weak coupling regime of the Coulomb branch. We use this map to translate wall crossing formulae and the no exotics theorem to statements about the Dirac/Dolbeault operators. The no exo...

  18. Frame junction vibration transmission with a modified frame deformation model.

    Science.gov (United States)

    Moore, J A

    1990-12-01

    A previous paper dealt with vibration transmission through junctions of connected frame members where the allowed frame deformations included bending, torsion, and longitudinal motions [J.A. Moore, J. Acoust. Soc. Am. 88, 2766-2776 (1990)]. In helicopter and aircraft structures the skin panels can constitute a high impedance connection along the length of the frames that effectively prohibits in-plane motion at the elevation of the skin panels. This has the effect of coupling in-plane bending and torsional motions within the frame. This paper discusses the transmission behavior through frame junctions that accounts for the in-plane constraint in idealized form by assuming that the attached skin panels completely prohibit inplane motion in the frames. Also, transverse shear deformation is accounted for in describing the relatively deep web frame constructions common in aircraft structures. Longitudinal motion in the frames is not included in the model. Transmission coefficient predictions again show the importance of out-of-plane bending deformation to the transmission of vibratory energy in an aircraft structure. Comparisons are shown with measured vibration transmission data along the framing in the overhead of a helicopter airframe, with good agreement. The frame junction description has been implemented within a general purpose statistical energy analysis (SEA) computer code in modeling the entire airframe structure including skin panels.

  19. Finite-dimensional Hilbert space and frame quantization

    Energy Technology Data Exchange (ETDEWEB)

    Cotfas, Nicolae [Faculty of Physics, University of Bucharest, PO Box 76-54, Post Office 76, Bucharest (Romania); Gazeau, Jean Pierre [Laboratoire APC, Universite Paris Diderot, 10, rue A Domon et L Duquet, 75205 Paris Cedex 13 (France); Vourdas, Apostol, E-mail: ncotfas@yahoo.com, E-mail: gazeau@apc.univ-paris7.fr, E-mail: A.Vourdas@bradford.ac.uk [Department of Computing, University of Bradford, Bradford BD7 1DP (United Kingdom)

    2011-04-29

    The quantum observables used in the case of quantum systems with finite-dimensional Hilbert space are defined either algebraically in terms of an orthonormal basis and discrete Fourier transformation or by using a continuous system of coherent states. We present an alternative approach to these important quantum systems based on the finite frame quantization. Finite systems of coherent states, usually called finite tight frames, can be defined in a natural way in the case of finite quantum systems. Novel examples of such tight frames are presented. The quantum observables used in our approach are obtained by starting from certain classical observables described by functions defined on the discrete phase space corresponding to the system. They are obtained by using a finite frame and a Klauder-Berezin-Toeplitz-type quantization. Semi-classical aspects of tight frames are studied through lower symbols of basic classical observables.

  20. Agenda-setting and Framing: details of the same theory?

    Directory of Open Access Journals (Sweden)

    Graça Penha Nascimento Rossetto

    2012-07-01

    Full Text Available In Agenda-setting and Framing literature there is a variety of research dedicated to examine the effects of communicative messages on the audience and its characteristics. In this context, there are theoretical approaches that alternate, coexist and complement each other. This article aims to make a historical review of them, analyzing and differentiating the attribute agenda setting and Framing, often seen as synonymous.

  1. Reading Columns

    OpenAIRE

    Coutts, Marion

    2008-01-01

    Reading Columns are twin permanent public sculptures commissioned as part of a £245m scheme for the redevelopment of the Chatham Place area in Reading. Dimensions: 3.5m high x 1.3m diameter each Field of knowledge: The work consists of twin bespoke columns of stainless steel and glass over digital colour transparencies. The piece revisits and reworks the idea of the Morris Column, a 19th C feature characteristic of major European metropolitan centres. A wraparound image on each of ...

  2. What's in a frame?

    DEFF Research Database (Denmark)

    Holmgreen, Lise-Lotte

    In the wake of the 2008 financial crisis, many large international banks received heavy criticism for their business conduct, which involved investments in dubious loans and business projects. For Denmark’s largest bank, Danske Bank, the situation was no different; it went from being considered...... from excellent to appalling within a number of months without it being able to benefit from its history of impeccable business conduct as the crisis hit (cf. Coombs 1995). In answering this question, I will argue that organisations are not operating in a social vacuum, but need to consider......; Lakoff 1993, 2003). Thus, in my presentation I will be concerned with the ways metaphor interact with other lexical items in the frames used by the Bank and the press to promote particular problem definitions and moral obligations, in turn assessing why these metaphors may or may not resonate...

  3. Does Extensive Reading Promote Reading Speed?

    Science.gov (United States)

    He, Mu

    2014-01-01

    Research has shown a wide range of learning benefits accruing from extensive reading. Not only is there improvement in reading, but also in a wide range of language uses and areas of language knowledge. However, few research studies have examined reading speed. The existing literature on reading speed focused on students' reading speed…

  4. Does Extensive Reading Promote Reading Speed?

    Science.gov (United States)

    He, Mu

    2014-01-01

    Research has shown a wide range of learning benefits accruing from extensive reading. Not only is there improvement in reading, but also in a wide range of language uses and areas of language knowledge. However, few research studies have examined reading speed. The existing literature on reading speed focused on students' reading speed without…

  5. Oral Reading Fluency in Second Language Reading

    Science.gov (United States)

    Jeon, Eun Hee

    2012-01-01

    This study investigated the role of oral reading fluency in second language reading. Two hundred and fifty-five high school students in South Korea were assessed on three oral reading fluency (ORF) variables and six other reading predictors. The relationship between ORF and other reading predictors was examined through an exploratory factor…

  6. WHAT IS READING?

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    Reading is enjoying,entertaining and ent ightening Reading is listening,speaking and writingReading is talking and discussing,with yourself with the author and with the others Reading is exploring,investigating and guessing. Reading is traveling backward and forward,historically and geographically. Reading is thinking in your own language,and/or in the other language. Reading is encoding and decoding. Reading is civilizing,rationalizing and intellectualizing. Reading is assimilating,associating,accumula...

  7. Reading Hygiene

    Institute of Scientific and Technical Information of China (English)

    耿一铭

    2006-01-01

    Here are some good points for good eye health that everyone can follow: 1.Rest your eyes before they get tired. Just close your eyes from time to time or look offat some distant object. 2.Do not read in either too dark a light or

  8. Round Robin Reading as a Teaching Method.

    Science.gov (United States)

    Hill, Charles H.

    1983-01-01

    Concludes that Round Robin Reading is a widely used method of teaching science and social studies in the intermediate grades. Discusses the effects of this method of instruction and provides alternatives for it. (FL)

  9. Seismic behavior and design of wall-EDD-frame systems

    Directory of Open Access Journals (Sweden)

    Oren eLavan

    2015-06-01

    Full Text Available Walls and frames have different deflection lines and, depending on the seismic mass they support, may often poses different natural periods. In many cases, wall-frame structures present an advantageous behavior. In these structures the walls and the frames are rigidly connected. Nevertheless, if the walls and the frames were not rigidly connected, an opportunity for an efficient passive control strategy would arise: Connecting the two systems by energy dissipation devices (EDDs to result in wall-EDD-frame systems. This, depending on the parameters of the system, is expected to lead to an efficient energy dissipation mechanism.This paper studies the seismic behavior of wall-EDD-frame systems in the context of retrofitting existing frame structures. The controlling non-dimensional parameters of such systems are first identified. This is followed by a rigorous and extensive parametric study that reveals the pros and cons of the new system versus wall-frame systems. The effect of the controlling parameters on the behavior of the new system are analyzed and discussed. Finally, tools are given for initial design of such retrofitting schemes. These enable both choosing the most appropriate retrofitting alternative and selecting initial values for its parameters.

  10. Beyond Cognition: Reading Motivation and Reading Comprehension

    OpenAIRE

    Wigfield, Allan; Gladstone, Jessica; Turci, Lara

    2016-01-01

    The authors review research on children’s reading motivation and its relation to their reading comprehension. They begin by discussing work on the development of school motivation in general and reading motivation in particular, reviewing work showing that many children’s reading motivation declines over the school years. Girls tend to have more positive motivation for reading than do boys, and there are ethnic differences in children’s reading motivation. Over the last 15 years researchers h...

  11. Alternative energies; Energies alternatives

    Energy Technology Data Exchange (ETDEWEB)

    Bonal, J.; Rossetti, P

    2007-07-01

    The earth took millions years to made the petroleum, the gas the coal and the uranium. Only a few centuries will be needed to exhaust these fossil fuels and some years to reach expensive prices. Will the wold continue on this way of energy compulsive consumption? The renewable energies and some citizen attitudes are sufficient to break this spiral. This book proposes to discuss these alternative energies. It shows that this attitude must be supported by the government. It takes stock on the more recent information concerning the renewable energies. it develops three main points: the electricity storage, the housing and the transports. (A.L.B.)

  12. Automatic Reading

    Institute of Scientific and Technical Information of China (English)

    胡迪

    2007-01-01

    <正>Reading is the key to school success and,like any skill,it takes practice.A child learns to walk by practising until he no longer has to think about how to put one foot in front of the other.The great athlete practises until he can play quickly,accurately and without thinking.Ed- ucators call it automaticity.

  13. 在毕赤酵母中利用口蹄疫病毒(FMDV)2A实现dTomato和串联MagaininⅡ基因的共表达%Coexpression of dTomato and Muti-copy Magainin Ⅱ Linked by the Foot-and mouth disease virus (FMDV) 2A Region in a Single Open Reading Frame in Pichia pastoris

    Institute of Scientific and Technical Information of China (English)

    卢艳敏; 崔海信; 王承民; 何宏轩

    2012-01-01

    抗菌肽(antibacterial peptides)是生物体内经诱导产生的一种具有生物活性的多肽,是天然免疫的重要组成部分.为了提高抗菌肽的表达量以及很方便地检测抗菌肽的表达情况,本研究利用口蹄疫病毒(Foot-and-mouth disease virus,FMDV) 2A将荧光蛋白dTomato基因和3个串联的MagaininⅡ基因融合到一个开放阅读框中(open reading frame,ORE),融合的基因被置于pPIC9K载体醇氧化酶基因(AOX1)启动子的下游,构建分泌型重组酵母表达载体pPIC9K-dTomato -2A-3M,将线性化的重组酵母表达载体通过电击法转入毕赤酵母(Pichia pastoris )GS115中,经G418筛选和PCR鉴定得到阳性转化子,然后将其转至摇瓶,在30℃、0.5%甲醇的条件下进行诱导表达,连续诱导3d.SDS-PAGE电泳图谱显示,在31 kD(dTomato)和9.5kD(3M)处有蛋白条带出现,在荧光显微镜下观察酵母表达上清发出红色荧光,对大肠杆菌(Escherichia coli)和金黄色葡萄球菌(Staphylococcus aureus)抑菌试验结果表明,重组抗菌肽串联的MagaininⅡ具有明显的抑菌效果.结果表明,由2A连接的融合基因(荧光蛋白dTomato和串联的MagaininⅡ)在毕赤酵母中成功的进行了表达,FMDV 2A在其C端剪切多聚蛋白,得到dTomato和串联的MagaininⅡ两个独立而有活性的蛋白,为小分子抗菌肽的表达提供一个高效的检测方法.%Antimicrobial peptideis is a kind of bioactive polypeptides induced by pathogens in organisms, which is an important component of innate immunity. In order to improve the expression of antimicrobial peptide and easily detect the expression of antimicrobial peptide. The red fluorescent protein dTomato and muti-copy Magainin II genes were fused into a single open reading frame (ORF) with a copy of the Foot-and mouth disease virus (FMDV) 2A region placed between the two genes. The fused genes were placed under the control of the alcohol oxidase (A 0X1) gene promoter in pPIC9K vector to construct the

  14. [The framing effect: medical implications].

    Science.gov (United States)

    Mazzocco, Ketti; Cherubini, Paolo; Rumiati, Rino

    2005-01-01

    Over the last 20 years, many studies explored how the way information is presented modifies choices. This sort of effect, referred to as "framing effects", typically consists of the inversion of choices when presenting structurally identical decision problems in different ways. It is a common assumption that physicians are unaffected (or less affected) by the surface description of a decision problem, because they are formally trained in medical decision making. However, several studies showed that framing effects occur even in the medical field. The complexity and variability of these effects are remarkable, making it necessary to distinguish among different framing effects, depending on whether the effect is obtained by modifying adjectives (attribute framing), goals of a behavior (goal framing), or the probability of an outcome (risky choice framing). A further reason for the high variability of the framing effects seems to be the domain of the decision problem, with different effects occurring in prevention decisions, disease-detection decisions, and treatment decisions. The present work reviews the studies on framing effects, in order to summarize them and clarify their possible role in medical decision making.

  15. Pairs of dual periodic frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Goh, Say Song

    2012-01-01

    is needed. The purpose of the present paper is to provide constructions of dual pairs of frames in the setting of the Hilbert space of periodic functions L2(0,2π). The frames constructed are given explicitly as trigonometric polynomials, which allows for an efficient calculation of the coefficients...

  16. Framing the patent troll debate.

    Science.gov (United States)

    Risch, Michael

    2014-02-01

    The patent troll debate has reached a fevered pitch in the USA. This editorial seeks to frame the debate by pointing out the lack of clarity in defining patent trolls and their allegedly harmful actions. It then frames the debate by asking currently unanswered questions: Where do troll patents come from? What are the effects of troll assertions? Will policy changes improve the system?

  17. Alternative additives; Alternative additiver

    Energy Technology Data Exchange (ETDEWEB)

    2007-08-15

    In this project a number of industrial and agricultural waste products have been characterised and evaluated in terms of alkali-getter performance. The intended use is for biomass-fired power stations aiming at reducing corrosion or slagging related problems. The following products have been obtained, characterised and evaluated: 1) Brewery draff 2) Danish de-gassed manure 3) Paper sludge 4) Moulding sand 5) Spent bleaching earth 6) Anorthosite 7) Sand 8) Clay-sludge. Most of the above alternative additive candidates are deemed unsuitable due to insufficient chemical effect and/or expensive requirements for pre-treatment (such as drying and transportation). 3 products were selected for full-scale testing: de-gassed manure, spent bleaching earth and clay slugde. The full scale tests were undertaken at the biomass-fired power stations in Koege, Slagelse and Ensted. Spent bleaching earth (SBE) and clay sludge were the only tested additive candidates that had a proven ability to react with KCl, to thereby reduce Cl-concentrations in deposits, and reduce the deposit flux to superheater tubes. Their performance was shown to nearly as good as commercial additives. De-gassed manure, however, did not evaluate positively due to inhibiting effects of Ca in the manure. Furthermore, de-gassed manure has a high concentration of heavy metals, which imposes a financial burden with regard to proper disposal of the ash by-products. Clay-sludge is a wet clay slurring, and drying and transportation of this product entails substantial costs. Spent bleaching does not require much pre-treatment and is therefore the most promising alternative additive. On the other hand, bleaching earth contains residual plant oil which means that a range of legislation relating to waste combustion comes into play. Not least a waste combustion fee of 330 DKK/tonne. For all alternative (and commercial) additives disposal costs of the increase ash by-products represents a significant cost. This is

  18. Reading Together: A Successful Reading Fluency Intervention

    Science.gov (United States)

    Young, Chase; Mohr, Kathleen A. J.; Rasinski, Timothy

    2015-01-01

    The article describes a reading fluency intervention called Reading Together that combines the method of repeated readings (Samuels, 1979) and the Neurological Impress Method (Heckelman, 1969). Sixteen volunteers from various backgrounds were recruited and trained to deliver the Reading Together intervention to struggling readers in third through…

  19. Reading with Maggie: The Effect of the Presence/Absence of a Classroom Pet Dog in a Reading Intervention Package

    Science.gov (United States)

    Bassette, Laura A.

    2011-01-01

    The purpose of this study was to examine if the presence/absence of a classroom pet dog impacted reading skills in four 5th grade middle school students with emotional/ behavioral disabilities. An alternating treatment design was used to assess the fluency and comprehension measures in students during reading a reading intervention package…

  20. Framed School--Frame Factors, Frames and the Dynamics of Social Interaction in School

    Science.gov (United States)

    Persson, Anders

    2015-01-01

    This paper aims to show how the Goffman frame perspective can be used in an analysis of school and education and how it can be combined, in such analysis, with the frame factor perspective. The latter emphasizes factors that are determined outside the teaching process, while the former stresses how actors organize their experiences and define…

  1. Seismic Performance Evaluation of Concentrically Braced Frames

    Science.gov (United States)

    Hsiao, Po-Chien

    Concentrically braced frames (CBFs) are broadly used as lateral-load resisting systems in buildings throughout the US. In high seismic regions, special concentrically braced frames (SCBFs) where ductility under seismic loading is necessary. Their large elastic stiffness and strength efficiently sustains the seismic demands during smaller, more frequent earthquakes. During large, infrequent earthquakes, SCBFs exhibit highly nonlinear behavior due to brace buckling and yielding and the inelastic behavior induced by secondary deformation of the framing system. These response modes reduce the system demands relative to an elastic system without supplemental damping. In design the re reduced demands are estimated using a response modification coefficient, commonly termed the R factor. The R factor values are important to the seismic performance of a building. Procedures put forth in FEMAP695 developed to R factors through a formalized procedure with the objective of consistent level of collapse potential for all building types. The primary objective of the research was to evaluate the seismic performance of SCBFs. To achieve this goal, an improved model including a proposed gusset plate connection model for SCBFs that permits accurate simulation of inelastic deformations of the brace, gusset plate connections, beams and columns and brace fracture was developed and validated using a large number of experiments. Response history analyses were conducted using the validated model. A series of different story-height SCBF buildings were designed and evaluated. The FEMAP695 method and an alternate procedure were applied to SCBFs and NCBFs. NCBFs are designed without ductile detailing. The evaluation using P695 method shows contrary results to the alternate evaluation procedure and the current knowledge in which short-story SCBF structures are more venerable than taller counterparts and NCBFs are more vulnerable than SCBFs.

  2. Mediernes framing af Folketingsvalget 2015

    OpenAIRE

    Jyrkinewsky Mathiesen, Marie; Fruergaard, Oliver; Toft-Nielsen, Nina Kristine; Løvstrup Thejls, Michelle; Abild Patrzarlek, Louise

    2015-01-01

    This project will be centered around the media’s framing of the political election in Denmark 2015. More specifically, we will examine how the public service channel DR1 has presented and framed the two political parties Alternativet and Det Kon-servative Folkeparti. Our examination will have its basis in framing theories as pre-sented by Robert Entman and Dietram A. Scheufele and also the theory of Social Constructivism. Through a deductive analysis, we have tried to answer whether DR1,...

  3. Approximation of the Inverse -Frame Operator

    Indian Academy of Sciences (India)

    M R Abdollahpour; A Najati

    2011-05-01

    In this paper, we introduce the concept of (strong) projection method for -frames which works for all conditional -Riesz frames. We also derive a method for approximation of the inverse -frame operator which is efficient for all -frames. We show how the inverse of -frame operator can be approximated as close as we like using finite-dimensional linear algebra.

  4. Framing as a Theory of Media Effects.

    Science.gov (United States)

    Scheufele, Dietram A.

    1999-01-01

    Systematizes the fragmented approaches to framing in political communication and integrates them into a comprehensive model. Classifies previous approaches to framing research along two dimensions: media frames versus audience frames; and the way frames are operationalized (independent variable or dependent variable). Identifies four key processes…

  5. Morphogenesis of Kinetic Reciprocal Frames

    DEFF Research Database (Denmark)

    Parigi, Dario; Sassone, Mario

    2011-01-01

    of interactive architecture (IA) started in the sixties of last century from the intersection between architecture and cybernetics. Recent studies were focused on static and kinematic determinacy of Reciprocal Frames, but their potential in the field of kinetic structures is still unexplored. Reciprocal Frames...... joins in intermediate points. In the present paper we will describe a new subset of kinetic structures based on reciprocal frames topology and pin-slot constraint, a connection where a pin is constrained to move along a slot, and to rotate around its axis. The first step in obtaining a kinetic...... reciprocal frame is to substitute in the intermediate connection the rotational hinges with pin-slot constraints. The second step it to determine the shape of the slot so as that the desired motion are compatible with the given constraints. The morphogenesis procedure is explained, in particular...

  6. Putting Safety in the Frame

    Directory of Open Access Journals (Sweden)

    Valerie Jean O’Keeffe

    2015-06-01

    Full Text Available Current patient safety policy focuses nursing on patient care goals, often overriding nurses’ safety. Without understanding how nurses construct work health and safety (WHS, patient and nurse safety cannot be reconciled. Using ethnography, we examine social contexts of safety, studying 72 nurses across five Australian hospitals making decisions during patient encounters. In enacting safe practice, nurses used “frames” built from their contextual experiences to guide their behavior. Frames are produced by nurses, and they structure how nurses make sense of their work. Using thematic analysis, we identify four frames that inform nurses’ decisions about WHS: (a communicating builds knowledge, (b experiencing situations guides decisions, (c adapting procedures streamlines work, and (d team working promotes safe working. Nurses’ frames question current policy and practice by challenging how nurses’ safety is positioned relative to patient safety. Recognizing these frames can assist the design and implementation of effective WHS management.

  7. Beyond Cognition: Reading Motivation and Reading Comprehension.

    Science.gov (United States)

    Wigfield, Allan; Gladstone, Jessica; Turci, Lara

    2016-09-01

    The authors review research on children's reading motivation and its relation to their reading comprehension. They begin by discussing work on the development of school motivation in general and reading motivation in particular, reviewing work showing that many children's reading motivation declines over the school years. Girls tend to have more positive motivation for reading than do boys, and there are ethnic differences in children's reading motivation. Over the last 15 years researchers have identified in both laboratory and classroom-based research instructional practices that positively impact students' reading motivation and ultimately their reading comprehension. There is a strong need for researchers to build on this work and develop and study in different age groups of children effective classroom-based reading motivation instructional programs for a variety of narrative and informational materials.

  8. Meta framing and polyphonic structures

    DEFF Research Database (Denmark)

    Pedersen, Karsten

    2015-01-01

    in various ways in BT’s 2012 coverage of a doping case involving Riis. In this article I investigate the way in which BT meta frames itself and its own actions in order to show and underline the seriousness with which BT treats sports journalism. The study is part of a recurring Danish project harvesting...... and mapping the entire news output in one specified week, and draws on insights from framing theory as well as polyphony theory....

  9. Frames and extension problems II

    DEFF Research Database (Denmark)

    Christensen, Ole; Kim, Hong Oh; Kim, Rae Young

    2014-01-01

    This article is a follow-up on the article Frames and Extension Problems I. Here we will go into more recent progress on the topic and also present some open problems.......This article is a follow-up on the article Frames and Extension Problems I. Here we will go into more recent progress on the topic and also present some open problems....

  10. Design of a very high frame rate camera based on an asynchronous CCD driving method

    Institute of Scientific and Technical Information of China (English)

    LI Bin-Kang; YANG Shao-Hua; GUO Ming-An; WANG gui-Lu; XIA Jing-Wao; LUO Tong-Ding

    2011-01-01

    A very high frame rate camera is designed based on an innovative CCD driving method. The CCD driving method is mainly implemented on frame transfer CCDs. Asynchronous drive timing sequences are applied in the image and storage section of the CCDs. Several rows of the charge in the image section are binned onto the same row in the storage section, and there are the same number of images to be stored in the storage section before they are read out. Based on the new driving method, the frame transfer CCDs can work at a very high frame rate in acquiring burst images though the reading speed remains at a lower level. A very high frame rate camera is designed in this paper. The innovative CCD driving method is mainly of concern. An e2v's CCD60 is adopted in the camera system, whose full size resolution is 128×128, and the up most frame rate is 1000 Hz in the conventional CCD driving method. By using the presented method, the CCD60 based imager is capable of operating at up to 40000 frames per second (fps) at a recognizable resolution of 128×32. Comparing cameras using traditional binning and region of interest technologies, the frame rate is normally less than 5000 fps while the resolution is only 32 × 32 lett.

  11. Differential Effects of Message Framing on Obesity Policy Support Between Democrats and Republicans.

    Science.gov (United States)

    Lee, Tae Kyoung; Kim, Hye Kyung

    2016-11-08

    This study tests whether gain- and loss-framed messages about establishing obesity-reducing policies have different persuasive effects on Republicans and Democrats. In a randomized between-subject experiment, participants (N = 384) read a message emphasizing either benefits to a society by establishing policies aimed to reduce obesity (i.e., gain-framed message) or costs to a society that fails to establish those policies (i.e., loss-framed message). Results indicated that Democrats perceived the gain-framed message as more persuasive than the loss-framed message and the perceived argument strength fully mediated the framing effect on Democrats' policy support; however, there was no framing effect on perceived argument strength among Republicans. On the other hand, the gain-framed message led Republicans to attribute the cause of obesity less to the individual level compared to the loss-framed message and the no-message condition. We observed no framing difference among Democrats on causal attributions. Theoretical and practical implications of these findings are discussed.

  12. Memory T-Cell-Mediated Immune Responses Specific to an Alternative Core Protein in Hepatitis C Virus Infection

    Science.gov (United States)

    Bain, Christine; Parroche, Peggy; Lavergne, Jean Pierre; Duverger, Blandine; Vieux, Claude; Dubois, Valérie; Komurian-Pradel, Florence; Trépo, Christian; Gebuhrer, Lucette; Paranhos-Baccala, Glaucia; Penin, François; Inchauspé, Geneviève

    2004-01-01

    In vitro studies have described the synthesis of an alternative reading frame form of the hepatitis C virus (HCV) core protein that was named F protein or ARFP (alternative reading frame protein) and includes a domain coded by the +1 open reading frame of the RNA core coding region. The expression of this protein in HCV-infected patients remains controversial. We have analyzed peripheral blood from 47 chronically or previously HCV-infected patients for the presence of T lymphocytes and antibodies specific to the ARFP. Anti-ARFP antibodies were detected in 41.6% of the patients infected with various HCV genotypes. Using a specific ARFP 99-amino-acid polypeptide as well as four ARFP predicted class I-restricted 9-mer peptides, we show that 20% of the patients display specific lymphocytes capable of producing gamma interferon, interleukin-10, or both cytokines. Patients harboring three different viral genotypes (1a, 1b, and 3) carried T lymphocytes reactive to genotype 1b-derived peptides. In longitudinal analysis of patients receiving therapy, both core and ARFP-specific T-cell- and B-cell-mediated responses were documented. The magnitude and kinetics of the HCV antigen-specific responses differed and were not linked with viremia or therapy outcome. These observations provide strong and new arguments in favor of the synthesis, during natural HCV infection, of an ARFP derived from the core sequence. Moreover, the present data provide the first demonstration of the presence of T-cell-mediated immune responses directed to this novel HCV antigen. PMID:15367612

  13. Bonobos and chimpanzees exhibit human-like framing effects.

    Science.gov (United States)

    Krupenye, Christopher; Rosati, Alexandra G; Hare, Brian

    2015-02-01

    Humans exhibit framing effects when making choices, appraising decisions involving losses differently from those involving gains. To directly test for the evolutionary origin of this bias, we examined decision-making in humans' closest living relatives: bonobos (Pan paniscus) and chimpanzees (Pan troglodytes). We presented the largest sample of non-humans to date (n = 40) with a simple task requiring minimal experience. Apes made choices between a 'framed' option that provided preferred food, and an alternative option that provided a constant amount of intermediately preferred food. In the gain condition, apes experienced a positive 'gain' event in which the framed option was initially presented as one piece of food but sometimes was augmented to two. In the loss condition, apes experienced a negative 'loss' event in which they initially saw two pieces but sometimes received only one. Both conditions provided equal pay-offs, but apes chose the framed option more often in the positive 'gain' frame. Moreover, male apes were more susceptible to framing than were females. These results suggest that some human economic biases are shared through common descent with other apes and highlight the importance of comparative work in understanding the origins of individual differences in human choice.

  14. Progressive Achievement Tests in Reading: Comprehension & Vocabulary. Teacher's Handbook. Second Edition.

    Science.gov (United States)

    Australian Council for Educational Research, Hawthorn.

    The teacher handbook for Progressive Achievement Tests (PATs) in Reading presents an overall description of these survey tests in reading comprehension and vocabulary knowledge for school years 3 to 9. There are two alternative forms of each test: (1) the Reading Comprehension tests are designed to measure two major aspects of reading skills…

  15. STRESS ANALYSIS OF BICYCLE FRAME

    Directory of Open Access Journals (Sweden)

    Mr.M.V.Pazare

    2014-06-01

    Full Text Available This paper deals with the stress analysis of bicycle frame by using Finite Element Method. The analysis is carried out in Ansys, The F.E.A. results are compared with theoretical results. In theoretical analysis the frame is treated as truss like structure and the stresses in various members of frame like top tube, down tube, seat tube, chain stay and seat stay are determined, considering various condition like, static start up, steady state paddling, vertical impact, horizontal impact, rear wheel braking. Also Finite Element Analysis is done considering the above conditions. From the analysis it is found that there is a good agreement between analytical and F.E.A. results. Result of all the cases reveals that maximum stress is found in top tube of the bicycle frame as compared to other frame members and is equal to 24.84 MPa which is less than yield strength in tension (i.e.Syt = 290 MPa for the material (aluminum T 6061 selected.

  16. Promoting Reading Motivation by Reading Together

    Science.gov (United States)

    Monteiro, Vera

    2013-01-01

    In the present project we tested the hypothesis that tutorial situations with peers would benefit children's reading motivation. Participants were from elementary school--80 fourth-graders and 80 second-graders. We used a questionnaire to assess reading motivation. In the tutorial sessions we developed a Paired Reading Program. The children who…

  17. Cosmetology Reading Strategies. 1980 Vocational Reading Series.

    Science.gov (United States)

    Thornton, L. Jay; And Others

    Cosmetology Reading Strategies is one of five instructional guides in the Reading Strategies in Vocational Education Series. Developed to assist teachers working with students considered disadvantaged because of reading deficiency, the guide contains several strategies, suitable for adaptation, specifically related to cosmetology instruction. Each…

  18. Reading(s) in the Writing Classroom.

    Science.gov (United States)

    Foster, David

    1997-01-01

    Interrogates the reading/writing connection by evaluating how three essays by published writers affected the attitude and writing practices of university students in a course on the personal essay. Describes the course. Suggests what findings imply for current rationales about the reading/writing connection and for the use of anthology readings in…

  19. EMPOWERING THE READING READABILITY

    Directory of Open Access Journals (Sweden)

    Handoko Handoko

    2014-06-01

    Full Text Available A general assumption about reading is that students improve their reading ability by reading a lot. This research was conducted to explain the use of extensive reading and aimed to figure out its implementation in improving students’ reading readability by using the class action research technique. The data of this research relates to the students ‘reading progress shown in their reading reports: spoken and written summary, reading comprehension and vocabulary mastery and their participation. The strategy was evolved in the continuity of reading. Students were encouraged to read extensively in and outside class. The findings indicated that the implementation could improve students’ reading readability.This attainment demonstrated that students’ reading readabilityis frosted through the continuity of reading. Other facts showed that students enjoyed reading. Students’ curiosity was also a significant factor. Their high curiosity explained why students continued reading though they realized that materials they read were difficult enough. Students’ self-confidence was also built as they were required to write a retelling story and to share their previous reading. Instead of their retelling and summarizing, students felt to be appreciated as readers. This appreciation indirectly helped students to improve the reading fondness.

  20. Cooperation, framing and political attitudes

    DEFF Research Database (Denmark)

    Fosgaard, Toke Reinholt; Hansen, Lars Gårn; Wengström, Erik Roland

    This paper shows that political attitudes are linked to cooperative behavior in an incentivized experiment with a large sample randomly drawn from the Danish population. However, this relationship depends on the way the experiment is framed. In the standard game in which subjects give to a public...... that this difference in the framing effect across political point of views is to some extent explained by differences in beliefs and basic cooperation preferences.......This paper shows that political attitudes are linked to cooperative behavior in an incentivized experiment with a large sample randomly drawn from the Danish population. However, this relationship depends on the way the experiment is framed. In the standard game in which subjects give to a public...

  1. Generating Virtual Images from Oblique Frames

    Directory of Open Access Journals (Sweden)

    Carlos R. T. Caldeira

    2013-04-01

    Full Text Available Image acquisition systems based on multi-head arrangement of digital cameras are attractive alternatives enabling a larger imaging area when compared to a single frame camera. The calibration of this kind of system can be performed in several steps or by using simultaneous bundle adjustment with relative orientation stability constraints. The paper will address the details of the steps of the proposed approach for system calibration, image rectification, registration and fusion. Experiments with terrestrial and aerial images acquired with two Fuji FinePix S3Pro cameras were performed. The experiments focused on the assessment of the results of self-calibrating bundle adjustment with and without relative orientation constraints and the effects to the registration and fusion when generating virtual images. The experiments have shown that the images can be accurately rectified and registered with the proposed approach, achieving residuals smaller than one pixel.

  2. Another frame, another game? : Explaining framing effects in economic games

    NARCIS (Netherlands)

    Gerlach, Philipp; Jaeger, B.; Hopfensitz, A.; Lori, E.

    2016-01-01

    Small changes in the framing of games (i.e., the way in which the game situation is described to participants) can have large effects on players' choices. For example, referring to a prisoner's dilemma game as the "Community Game" as opposed to the "Wall Street Game" can double the cooperation rate

  3. Effects of message framing on self-report and accelerometer-assessed physical activity across age and gender groups.

    Science.gov (United States)

    Li, Kin-Kit; Cheng, Sheung-Tak; Fung, Helene H

    2014-02-01

    This study compared message-framing effects on physical activity (PA) across age and gender groups. Participants included 111 younger and 100 older adults (68% were women), randomly assigned to read gain-framed or loss-framed PA messages in promotion pamphlets, and who wore accelerometers for the following 14 days. Using regression analyses controlling for demographic and health factors, we found significant age-by-gender-by-framing interactions predicting self-report (B = -4.39, p = .01) and accelerometer-assessed PA (B = -2.44, p = .02) during the follow-up period. Gain-framed messages were more effective than loss-framed messages in promoting PA behaviors only among older men. We speculated that the age-related positivity effect, as well as the age and gender differences in issue involvement, explained the group differences in framing. In addition, more time availability and higher self-efficacy among older men might have contributed to the results.

  4. Differential reading recovery in patients with severe to moderate closed head injury.

    Science.gov (United States)

    Kaplan, C P

    1990-12-01

    A differential recovery was seen when alternate forms of a nationally standardized test of Reading Vocabulary, Literal Reading Comprehension and Inferential Reading Comprehension was administered serially to 10 consecutive closed head injury admissions to a university rehabilitation hospital. Inferential Reading Comprehension was the most impaired and had the fastest recovery rate. Subtle cognitive deficits in Inferential Reading Comprehension were detected when Reading Vocabulary was at or better than a twelfth grade level. Maximal recovery of all three reading functions occurred within 4 months after trauma, with most occurring in the first 3 months. The reading recovery pattern parallels the recovery of intelligence scores in the literature.

  5. 49 CFR 393.201 - Frames.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Frames. 393.201 Section 393.201 Transportation... SAFE OPERATION Frames, Cab and Body Components, Wheels, Steering, and Suspension Systems § 393.201 Frames. (a) The frame or chassis of each commercial motor vehicle shall not be cracked, loose, sagging...

  6. Sparsity and spectral properties of dual frames

    DEFF Research Database (Denmark)

    Krahmer, Felix; Kutyniok, Gitta; Lemvig, Jakob

    2013-01-01

    We study sparsity and spectral properties of dual frames of a given finite frame. We show that any finite frame has a dual with no more than $n^2$ non-vanishing entries, where $n$ denotes the ambient dimension, and that for most frames no sparser dual is possible. Moreover, we derive an expressio...

  7. Framing and misperception in public good experiments

    DEFF Research Database (Denmark)

    Fosgaard, Toke Reinholt; Hansen, Lars Gårn; Wengström, Erik Roland

    2017-01-01

    in misperceptions are linked to the framing effect on subjects' cooperation behavior. When we do not control for the different levels of misperceptions between frames, we observe a significant framing effect on subjects’ cooperation preferences. However, this framing effect becomes insignificant once we remove......Earlier studies have found that framing has substantial impact on the degree of cooperation observed in public good experiments. We show that the way the public good game is framed affects misperceptions about the incentives of the game. Moreover, we show that such framing-induced differences...

  8. Stability of (p, Y)-Operator Frames

    Institute of Scientific and Technical Information of China (English)

    Zhi Hua GUO; Huai Xin CAO; Jun Cheng YIN

    2011-01-01

    In this paper we study the stability of (p, Y)-operator frames. We firstly discuss the relations between p-Bessel sequences (or p-frames) and (p, Y)-operator Bessel sequences (or (p, Y)-operator frames). Through defining a new union, we prove that adding some elements to a given (p, Y)-operator frame, the resulted sequence will be still a (p, Y)-operator frame. We obtain a necessary and sufficient condition for a sequence of compound operators to be a (p, Y)operator frame. Lastly, we show that (p, Y)-operator frames for X are stable under some small perturbations.

  9. Lip reading using neural networks

    Science.gov (United States)

    Kalbande, Dhananjay; Mishra, Akassh A.; Patil, Sanjivani; Nirgudkar, Sneha; Patel, Prashant

    2011-10-01

    Computerized lip reading, or speech reading, is concerned with the difficult task of converting a video signal of a speaking person to written text. It has several applications like teaching deaf and dumb to speak and communicate effectively with the other people, its crime fighting potential and invariance to acoustic environment. We convert the video of the subject speaking vowels into images and then images are further selected manually for processing. However, several factors like fast speech, bad pronunciation, and poor illumination, movement of face, moustaches and beards make lip reading difficult. Contour tracking methods and Template matching are used for the extraction of lips from the face. K Nearest Neighbor algorithm is then used to classify the 'speaking' images and the 'silent' images. The sequence of images is then transformed into segments of utterances. Feature vector is calculated on each frame for all the segments and is stored in the database with properly labeled class. Character recognition is performed using modified KNN algorithm which assigns more weight to nearer neighbors. This paper reports the recognition of vowels using KNN algorithms

  10. Reading Assessment: Looking Ahead

    Science.gov (United States)

    Afflerbach, Peter

    2016-01-01

    In this article, I focus on three areas of reading assessment that I believe to be crucial for students' reading development: developing comprehensive formative assessments, assessing the wide array of factors that contribute to students' reading development, and fostering student independence by helping students learn to use reading assessment on…

  11. Epistemic Frames for Epistemic Games

    Science.gov (United States)

    Shaffer, David W.

    2006-01-01

    This paper, develops the concept of "epistemic frames" as a mechanism through which students can use experiences in video games, computer games, and other interactive learning environments to help them deal more effectively with situations outside of the original context of learning. Building on ideas of "islands of expertise" [Crowley, K., &…

  12. Wavelet frames and their duals

    DEFF Research Database (Denmark)

    Lemvig, Jakob

    2008-01-01

    structure. The dilation of the wavelet building blocks in higher dimension is done via a square matrix which is usually taken to be integer valued. In this thesis we step away from the "usual" integer, expansive dilation and consider more general, expansive dilations. In most applications of wavelet frames...

  13. Gold plating on spectacle frames.

    Science.gov (United States)

    Kenny, I; Mitchell, J W; Walsh, G

    1997-05-01

    An investigation was carried out into the thickness and standard of application of the plating and lacquer coatings applied to three metal spectacle frames. All conform to BS 6625 (1991) for plating thickness, but there was considerable variation in regularity and porosity.

  14. Frame Rate and Human Vision

    Science.gov (United States)

    Watson, Andrew B.

    2012-01-01

    To enhance the quality of the theatre experience, the film industry is interested in achieving higher frame rates for capture and display. In this talk I will describe the basic spatio-temporal sensitivities of human vision, and how they respond to the time sequence of static images that is fundamental to cinematic presentation.

  15. Media framing and social movements

    NARCIS (Netherlands)

    Vliegenthart, R.; Snow, D.A.; Della Porta, D.; Klandermans, B.; McAdam, D.

    2013-01-01

    In their study of media content, mass communication scholars commonly rely on Entman's (1993: 52) definition of framing: "[selecting] some aspects of a perceived reality and make them more salient in a communicating text, in such a way as to promote a particular problem definition, causal interpreta

  16. Framing Land-use Dynamics

    NARCIS (Netherlands)

    Verburg, P.H.; Veldkamp, A.; Dijst, M.J.; Schot, P.

    2003-01-01

    Framing land use dynamics was organised as part of the Networks in the Delta research programme of Utrecht which is a LUCC endorsed activity since August 2001. The programme aims at developing a generic theoretical and methodeological framework for understanding and modelling the complex interaction

  17. Fight or flight: Affective news framing effects

    OpenAIRE

    Feinholdt, A.

    2016-01-01

    This dissertation investigates how emotions shape and are shaped by news framing and how this interplay leads to a number of affective news framing effects. My work reveals that emotions do not only function as mechanisms but also as channels strengthening or weakening framing effects. In addition, I show that issue characteristics and the selected news frame can determine whether and how emotions are elicited. Further, I provide evidence that affective news framing effects can arise as the r...

  18. On Efficient Reading

    Institute of Scientific and Technical Information of China (English)

    陈伟平

    2003-01-01

    Time is limited for each reader,but many readers waste a lot oftime on unimportant things, and they read everything at the same speed and in the same way. As a result, they often fail to understand the word and the sentence; they don't know how one sentence relates to another, and how the whole text fixes together. They are not reading efficiently. It is high time that we held a discussion on efficient reading. The author states that efficient reading involves adequate comprehension with appropriate reading rate. Pointing out the factors that influence reading rate and comprehension, this article put forward some suggestions on efficient reading.

  19. The missing link: audience frames in the process model of framing effects

    NARCIS (Netherlands)

    van Drunen, A.S.; Vliegenthart, R.; Boomgaarden, H.G.; de Vreese, C.H.

    2011-01-01

    The process model of framing effects proposes that media frames affect public opinion via audience frames. However, there is a lack of research examining these audience frames. In this investigation we define and subsequently develop an empirical measurement of audience frames, which is applied to t

  20. Relevance of context and time-frame in bursty dynamics

    CERN Document Server

    Jo, Hang-Hyun; Perotti, Juan I; Kaski, Kimmo

    2013-01-01

    Inhomogeneous temporal processes in natural and social phenomena have been described by bursts that are rapidly occurring events within short periods alternating with long periods of low activity. Such a temporal process can be decomposed into sub-processes, according to the contexts, i.e. circumstances in which the events occur. Then contextual bursts for each sub-process are related to context-blind bursts for the original process. This requires to study contextual bursts in real time-frame as well as in ordinal time-frame, where the real timings of events are replaced by their orders in the event sequence. By analyzing a model of uncorrelated inter-event times we find that contextual bursts in real time-frame can be dominated by either context-blind bursts or contextual bursts in ordinal time-frame, or be characterized by both. These results on the relevance of context and time-frame give insight into the origin of bursts.

  1. What Oral Text Reading Fluency Can Reveal about Reading Comprehension

    Science.gov (United States)

    Veenendaal, Nathalie J.; Groen, Margriet A.; Verhoeven, Ludo

    2015-01-01

    Text reading fluency--the ability to read quickly, accurately and with a natural intonation--has been proposed as a predictor of reading comprehension. In the current study, we examined the role of oral text reading fluency, defined as text reading rate and text reading prosody, as a contributor to reading comprehension outcomes in addition to…

  2. Ultra-fast framing camera tube

    Science.gov (United States)

    Kalibjian, Ralph

    1981-01-01

    An electronic framing camera tube features focal plane image dissection and synchronized restoration of the dissected electron line images to form two-dimensional framed images. Ultra-fast framing is performed by first streaking a two-dimensional electron image across a narrow slit, thereby dissecting the two-dimensional electron image into sequential electron line images. The dissected electron line images are then restored into a framed image by a restorer deflector operated synchronously with the dissector deflector. The number of framed images on the tube's viewing screen is equal to the number of dissecting slits in the tube. The distinguishing features of this ultra-fast framing camera tube are the focal plane dissecting slits, and the synchronously-operated restorer deflector which restores the dissected electron line images into a two-dimensional framed image. The framing camera tube can produce image frames having high spatial resolution of optical events in the sub-100 picosecond range.

  3. Sparsity and spectral properties of dual frames

    CERN Document Server

    Krahmer, Felix; Lemvig, Jakob

    2012-01-01

    We study sparsity and spectral properties of dual frames of a given finite frame. We show that any finite frame has a dual with no more than $n^2$ non-vanishing entries, where $n$ denotes the ambient dimension, and that for most frames no sparser dual is possible. Moreover, we derive an expression for the exact sparsity level of the sparsest dual for any given finite frame using a generalized notion of spark. We then study the spectral properties of dual frames in terms of singular values of the synthesis operator. We provide a complete characterization for which spectral patterns of dual frames are possible for a fixed frame. For many cases, we provide simple explicit constructions for dual frames with a given spectrum, in particular, if the constraint on the dual is that it be tight.

  4. Teaching Children to Read : The Fragile Link Between Science & Federal Education Policy

    OpenAIRE

    Gregory Camilli; Sadako Vargas; Michele Yurecko

    2003-01-01

    Teaching Children to Read (TCR) has stirred much controversy among reading experts regarding the efficacy of phonics instruction. This report, which was conducted by the National Reading Panel (NRP), has also played an important role in subsequent federal policy regarding reading instruction. Using meta-analysis, the NRP found that systematic phonics instruction was more effective than alternatives in teaching children to read. In the present study, the findings and procedures leading to TCR ...

  5. Variance based OFDM frame synchronization

    Directory of Open Access Journals (Sweden)

    Z. Fedra

    2012-04-01

    Full Text Available The paper deals with a new frame synchronization scheme for OFDM systems and calculates the complexity of this scheme. The scheme is based on the computing of the detection window variance. The variance is computed in two delayed times, so a modified Early-Late loop is used for the frame position detection. The proposed algorithm deals with different variants of OFDM parameters including guard interval, cyclic prefix, and has good properties regarding the choice of the algorithm's parameters since the parameters may be chosen within a wide range without having a high influence on system performance. The verification of the proposed algorithm functionality has been performed on a development environment using universal software radio peripheral (USRP hardware.

  6. Developing the Framed Standard Model

    CERN Document Server

    Baker, Michael J; Chan, H M; Tsou, S T

    2011-01-01

    The framed standard model (FSM) suggested earlier, which incorporates the Higgs field and 3 fermion generations as part of the framed gauge theory structure, is here developed further to show that it gives both quarks and leptons hierarchical masses and mixing matrices akin to what is experimentally observed. Among its many distinguishing features which lead to the above results are (i) the vacuum is degenerate under a global $su(3)$ symmetry which plays the role of fermion generations, (ii) the fermion mass matrix is "universal", rank-one and rotates (changes its orientation in generation space) with changing scale $\\mu$, (iii) the metric in generation space is scale-dependent too, and in general non-flat, (iv) the theta-angle term in the QCD action of topological origin gets transformed into the CP-violating phase of the CKM matrix for quarks, thus offering at the same time a solution to the strong CP problem.

  7. Alternative metrics

    Science.gov (United States)

    2012-11-01

    As the old 'publish or perish' adage is brought into question, additional research-impact indices, known as altmetrics, are offering new evaluation alternatives. But such metrics may need to adjust to the evolution of science publishing.

  8. Classifying tight Weyl-Heisenberg frames

    DEFF Research Database (Denmark)

    Cazsazza, P.; Janssen, A. J. E. M.; Christensen, Ole

    1999-01-01

    A Weyl-Heisenberg frame for L^2(R) is a frame consisting of translates and modulates of a fixed function. In this paper we give necessary and sufficient conditions for this family to form a tight WH-frame. This allows us to write down explicitly all functions g for which all translates and modula......A Weyl-Heisenberg frame for L^2(R) is a frame consisting of translates and modulates of a fixed function. In this paper we give necessary and sufficient conditions for this family to form a tight WH-frame. This allows us to write down explicitly all functions g for which all translates...

  9. Perturbation of Wavelet and Gabor Frames

    Institute of Scientific and Technical Information of China (English)

    Ivana Carrizo; Sergio Favier

    2003-01-01

    In this work two aspects of theory of frames are presented: a side necessary condition on irregular wavelet frames is obtained, another perturbation of wavelet and Gabor frames is considered. Specifically,we present the results obtained on frame stability when one disturbs the mother of wavelet frame, or the parameter of dilatation, and in Gabor frames when the generating function or the parameter of translation are perturbed. In all cases we work without demanding compactness of the support, neither on the generating function, nor on its Fourier transform.

  10. Orthogonal Multiwavelet Frames in L2Rd

    Directory of Open Access Journals (Sweden)

    Liu Zhanwei

    2012-01-01

    Full Text Available We characterize the orthogonal frames and orthogonal multiwavelet frames in L2Rd with matrix dilations of the form (Df(x=detAf(Ax, where A is an arbitrary expanding d×d matrix with integer coefficients. Firstly, through two arbitrarily multiwavelet frames, we give a simple construction of a pair of orthogonal multiwavelet frames. Then, by using the unitary extension principle, we present an algorithm for the construction of arbitrarily many orthogonal multiwavelet tight frames. Finally, we give a general construction algorithm for orthogonal multiwavelet tight frames from a scaling function.

  11. Cultural Factors in Reading

    Institute of Scientific and Technical Information of China (English)

    孔敏

    2005-01-01

    Reading is a basic ability in learning English and reading comprehension exercise is a common way to assess this ability.Since reading is a communicative activity between author and reader in written form,there are some different rules and regulations of this communication in different countries.Therefore,cultural factors,existing in reading,decide,help,and influence the percentage of the right answers.This article attempts to analyze the effects of cultural differences in reading and the barriers in comprehension,and aims to improve students awareness of cultural differences in reading.

  12. Deletion of Dystrophin In-Frame Exon 5 Leads to a Severe Phenotype: Guidance for Exon Skipping Strategies.

    Directory of Open Access Journals (Sweden)

    Zhi Yon Charles Toh

    Full Text Available Duchenne and Becker muscular dystrophy severity depends upon the nature and location of the DMD gene lesion and generally correlates with the dystrophin open reading frame. However, there are striking exceptions where an in-frame genomic deletion leads to severe pathology or protein-truncating mutations (nonsense or frame-shifting indels manifest as mild disease. Exceptions to the dystrophin reading frame rule are usually resolved after molecular diagnosis on muscle RNA. We report a moderate/severe Becker muscular dystrophy patient with an in-frame genomic deletion of DMD exon 5. This mutation has been reported by others as resulting in Duchenne or Intermediate muscular dystrophy, and the loss of this in-frame exon in one patient led to multiple splicing events, including omission of exon 6, that disrupts the open reading frame and is consistent with a severe phenotype. The patient described has a deletion of dystrophin exon 5 that does not compromise recognition of exon 6, and although the deletion does not disrupt the reading frame, his clinical presentation is more severe than would be expected for classical Becker muscular dystrophy. We suggest that the dystrophin isoform lacking the actin-binding sequence encoded by exon 5 is compromised, reflected by the phenotype resulting from induction of this dystrophin isoform in mouse muscle in vivo. Hence, exon skipping to address DMD-causing mutations within DMD exon 5 may not yield an isoform that confers marked clinical benefit. Additional studies will be required to determine whether multi-exon skipping strategies could yield more functional dystrophin isoforms, since some BMD patients with larger in-frame deletions in this region have been reported with mild phenotypes.

  13. I spy with my little eye: cognitive processing of framed physical activity messages.

    Science.gov (United States)

    Bassett-Gunter, Rebecca L; Latimer-Cheung, Amy E; Martin Ginis, Kathleen A; Castelhano, Monica

    2014-01-01

    The primary purpose was to examine the relative cognitive processing of gain-framed versus loss-framed physical activity messages following exposure to health risk information. Guided by the Extended Parallel Process Model, the secondary purpose was to examine the relation between dwell time, message recall, and message-relevant thoughts, as well as perceived risk, personal relevance, and fear arousal. Baseline measures of perceived risk for inactivity-related disease and health problems were administered to 77 undergraduate students. Participants read population-specific health risk information while wearing a head-mounted eye tracker, which measured dwell time on message content. Perceived risk was then reassessed. Next, participants read PA messages while the eye tracker measured dwell time on message content. Immediately following message exposure, recall, thought-listing, fear arousal, and personal relevance were measured. Dwell time on gain-framed messages was significantly greater than loss-framed messages. However, message recall and thought-listing did not differ by message frame. Dwell time was not significantly related to recall or thought-listing. Consistent with the Extended Parallel Process Model, fear arousal was significantly related to recall, thought-listing, and personal relevance. In conclusion, gain-framed messages may evoke greater dwell time than loss-famed messages. However, dwell time alone may be insufficient for evoking further cognitive processing.

  14. Frames of exponentials:lower frame bounds for finite subfamilies, and approximation of the inverse frame operator

    DEFF Research Database (Denmark)

    Christensen, Ole; Lindner, Alexander M

    2001-01-01

    We give lower frame bounds for finite subfamilies of a frame of exponentials {e(i lambdak(.))}k is an element ofZ in L-2(-pi,pi). We also present a method for approximation of the inverse frame operator corresponding to {e(i lambdak(.))}k is an element ofZ, where knowledge of the frame bounds for...... for finite subfamilies is crucial. (C) 2001 Elsevier Science Inc. All rights reserved....

  15. Incorporating RTI in a Hybrid Model of Reading Disability

    OpenAIRE

    2014-01-01

    The present study seeks to evaluate a hybrid model of identification that incorporates response-to-intervention (RTI) as a one of the key symptoms of reading disability. The one-year stability of alternative operational definitions of reading disability was examined in a large scale sample of students who were followed longitudinally from first to second grade. The results confirmed previous findings of limited stability for single-criterion based operational definitions of reading disability...

  16. Effects of a Student-Reads-Aloud Accommodation on the Performance of Students with and without Learning Disabilities on a Test of Reading Comprehension

    Science.gov (United States)

    Elbaum, Batya; Arguelles, Maria Elena; Campbell, Yvonne; Saleh, Maya Bardawil

    2004-01-01

    In this study, we investigated the impact of a student-reads-aloud accommodation on the performance of middle school and high school students with and without learning disabilities (LD) on a test of reading comprehension. Data for the analyses came from 311 students (n = 230 with LD) who took alternate forms of a reading test in a standard and an…

  17. To read or not to read

    NARCIS (Netherlands)

    Mol, Suzanne Elizabeth

    2010-01-01

    There is a widely held belief that reading (story)books makes us smarter and helps promote success in life. Does scientific evidence support this notion? The three meta-analyses in this thesis comprise 146 studies between 1988 and 2010 (N=10,308 participants) that addressed the role of book reading

  18. Lights, Camera, Read! Arizona Reading Program Manual.

    Science.gov (United States)

    Arizona State Dept. of Library, Archives and Public Records, Phoenix.

    This document is the manual for the Arizona Reading Program (ARP) 2003 entitled "Lights, Camera, Read!" This theme spotlights books that were made into movies, and allows readers to appreciate favorite novels and stories that have progressed to the movie screen. The manual consists of eight sections. The Introduction includes welcome letters from…

  19. Incorporating RTI in a Hybrid Model of Reading Disability

    Science.gov (United States)

    Spencer, Mercedes; Wagner, Richard K.; Schatschneider, Christopher; Quinn, Jamie M.; Lopez, Danielle; Petscher, Yaacov

    2014-01-01

    The present study seeks to evaluate a hybrid model of identification that incorporates response to instruction and intervention (RTI) as one of the key symptoms of reading disability. The 1-year stability of alternative operational definitions of reading disability was examined in a large-scale sample of students who were followed longitudinally…

  20. Stress Matters: Effects of Anticipated Lexical Stress on Silent Reading

    Science.gov (United States)

    Breen, Mara; Clifton, Charles, Jr.

    2011-01-01

    This paper presents findings from two eye-tracking studies designed to investigate the role of metrical prosody in silent reading. In Experiment 1, participants read stress-alternating noun-verb or noun-adjective homographs (e.g. "PREsent", "preSENT") embedded in limericks, such that the lexical stress of the homograph, as determined by context,…

  1. TWO CONDITIONS FOR NONUNIFORM GABOR FRAMES

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A necessary condition is given for general nonuniform Gabor frames, which generalizes Benedetto and Walnut's theorem. A sufficient and necessary condition for a class of nonuniform Gabor frames is proved.

  2. An Analysis of the Discourse and Actions of Reading Conferences with English Learners: A Situated Perspective

    Science.gov (United States)

    Brown, Sally

    2013-01-01

    In this article the author shares examples of reading conferences with 10 English learners over the course of a semester. The reading conferences are conducted by four monolingual, European-American teachers at a primary school in the Southeastern United States. Sociocultural and identity theories are used as a theoretical frame for the research…

  3. Reading "Salt and Pepper": Social Practices, Unfinished Narratives, and Critical Interpretations

    Science.gov (United States)

    Anderson, Diane Downer

    2008-01-01

    In "Reading "Salt and Pepper"" Anderson examines a story written by three third grade girls and their insights about that story as they re-read it during its production and retrospectively, eight years later. Using a frame for understanding children's writing as social practice, the children's interviews, showing their multiple and sometimes…

  4. On English Reading Skills

    Institute of Scientific and Technical Information of China (English)

    钱芬

    2008-01-01

    Reading is one of the four important skills in English learning.It is also a skill that the students need to possess to support independent and self-directed learning.With the development of society,science and technology develop at hish speed and the competition in the society become sharp.Reading is a way for students to be more knowledgeable and successful.So,it becomes more and more important to speed up their reading in order to acquire as much information as possible.Thus,fostering a good English reading habit is essential,and being able to adopt different reading skills for different reading materials and purposes will also help to read more effectively.The paper mainly concenls some basic English reading skills.

  5. Can Reading Help?

    Science.gov (United States)

    Crowe, Chris

    2003-01-01

    Ponders the effect of September 11th on teenagers. Proposes that reading books can help teenagers sort out complicated issues. Recommends young adult novels that offer hope for overcoming tragedy. Lists 50 short story collections worth reading. (PM)

  6. Teaching Reading Skills

    Institute of Scientific and Technical Information of China (English)

    刘恒

    2014-01-01

    Reading skills are very important part in language teaching and learning. This paper is written after attending lectures given by an Australian teacher named Rod Ellis focusing on how to teach reading skills using authentic materials.

  7. Analisis Framing dalam Riset Public Relations

    OpenAIRE

    NARAYANA MAHENDRA PRASTYA

    2016-01-01

    This paper aims to give description about how to use frame analysis in Public Relations (PR) research. The author use two framing models: Entman and Pan & Kosicki. The object is organization official statement about particular issue. Frame analysis method rarely used in Public Relations research. This methods commonly use in journalism study, to analyse the news in media. Meanwhile, the key word of framing is the social construction of reality. Organization can make social cons...

  8. A Class of Bidimensional FMRA Wavelet Frames

    Institute of Scientific and Technical Information of China (English)

    Yun Zhang LI

    2006-01-01

    This paper addresses the construction of wavelet frame from a frame multiresolution analysis (FMRA) associated with a dilation matrix of determinant ±2. The dilation matrices of determinant ±2 can be classified as six classes according to integral similarity. In this paper, for four classes of them, the construction of wavelet frame from an FMRA is obtained, and, as examples, Shannon type wavelet frames are constructed, which have an independent value for their optimality in some sense.

  9. Pupil Decision Making in the Reading Curriculum.

    Science.gov (United States)

    Ediger, Marlow

    Being able to make decisions is important for all students. Students need to have opportunities to choose from among alternative situations. Reading, as one curriculum area, provides a plethora of opportunities to choose and to select. The philosopher John Locke believed the following facets of an individual's development were in the ensuing order…

  10. Reading through Linguistic Borderlands: Latino Students' Transactions with Narrative Texts

    Science.gov (United States)

    Martinez-Roldan, Carmen; Sayer, Peter

    2006-01-01

    This study examines the biliteracy development of a group of bilingual Latino third graders in an elementary school in the south-west USA. It focuses on the role of language in children's reading comprehension of narrative texts in Spanish and English in a school context. The authors frame their analysis within the "Continua of Biliteracy" model…

  11. Tactics for Reading Comprehension

    Institute of Scientific and Technical Information of China (English)

    孔祥航; 张艳荣

    2003-01-01

    In recent years, reading comprehension is taking up a larger and larger part in almost every international test or domestic examination. Knowing the basic knowledge and grasping the test - taking tactics are key factors of good reading comprehension. In this thesis, I will dwell on nine commonly used tactics for reading comprehension. This will help you to deal with the problems with reading comprehension efficiently.

  12. Computable Frames in Computable Banach Spaces

    Directory of Open Access Journals (Sweden)

    S.K. Kaushik

    2016-06-01

    Full Text Available We develop some parts of the frame theory in Banach spaces from the point of view of Computable Analysis. We define computable M-basis and use it to construct a computable Banach space of scalar valued sequences. Computable Xd frames and computable Banach frames are also defined and computable versions of sufficient conditions for their existence are obtained.

  13. Fight or flight: Affective news framing effects

    NARCIS (Netherlands)

    Feinholdt, A.

    2016-01-01

    This dissertation investigates how emotions shape and are shaped by news framing and how this interplay leads to a number of affective news framing effects. My work reveals that emotions do not only function as mechanisms but also as channels strengthening or weakening framing effects. In addition,

  14. Influence of framing on medical decision making

    OpenAIRE

    Feng, Jun; Gong, Jingjing; Huang, Yonghua; Wei, Yazhou; Zhang, Weiwei; Zhang, Yan

    2013-01-01

    Numerous studies have demonstrated the robustness of the framing effect in a variety of contexts, especially in medical decision making. Unfortunately, research is still inconsistent as to how so many variables impact framing effects in medical decision making. Additionally, much attention should be paid to the framing effect not only in hypothetical scenarios but also in clinical experience.

  15. Getting real: the duration of framing effects

    NARCIS (Netherlands)

    Lecheler, S.; de Vreese, C.H.

    2011-01-01

    A growing number of studies test the effects of news framing on citizens’ understanding of politics. By employing experimental designs, these studies report significant effects for a multitude of issues and frames. However, what happens to the framing effect after initial exposure? Based on a ‘‘clas

  16. Mechanical Energy Change in Inertial Reference Frames

    Science.gov (United States)

    Ghanbari, Saeed

    2016-01-01

    The mechanical energy change of a system in an inertial frame of reference equals work done by the total nonconservative force in the same frame. This relation is covariant under the Galilean transformations from inertial frame S to S', where S' moves with constant velocity relative to S. In the presence of nonconservative forces, such as normal…

  17. Common Frame of Reference and social justice

    NARCIS (Netherlands)

    Hesselink, M.W.; Satyanarayana, R.

    2009-01-01

    The article "Common Frame of Reference and Social Justice" by Martijn W. Hesselink evaluates the Draft Common Frame of Reference (DCFR) of social justice. It discusses the important areas, namely a common frame of Reference in a broad sense, social justice and contract law, private law and democracy

  18. A Framed Temporal Logic Programming Language

    Institute of Scientific and Technical Information of China (English)

    Zhen-Hua Duan; Maciej Koutny

    2004-01-01

    We discuss the projection temporal logic (PTL), based on a primitive projection operator, prj. A framing technique is also presented, using which a synchronization operator, await, is defined within the underlying logic. A framed temporal logic programming language (FTLL) is presented. To illustrate how to use both the language and framing technique, some examples are given.

  19. 10 CFR 710.35 - Time frames.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 4 2010-01-01 2010-01-01 false Time frames. 710.35 Section 710.35 Energy DEPARTMENT OF... Matter or Special Nuclear Material Miscellaneous § 710.35 Time frames. Statements of time established for processing aspects of a case under this subpart are the agency's desired time frames in implementing...

  20. Irregular wavelet frames on L2(Rn)

    Institute of Scientific and Technical Information of China (English)

    YANG Deyun; ZHOU Xingwei

    2005-01-01

    In this paper, we present the conditions on dilation parameter {sj }j that ensure a discrete irregular wavelet system {Snj/2ψ(sj·-bk)}j∈(Z),k∈(Z)n to be a frame on L2(Rn),and for the wavelet frame we consider the perturbations of translation parameter b and frame function ψ respectively.

  1. 21 CFR 886.5842 - Spectacle frame.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false Spectacle frame. 886.5842 Section 886.5842 Food... DEVICES OPHTHALMIC DEVICES Therapeutic Devices § 886.5842 Spectacle frame. (a) Identification. A spectacle frame is a device made of metal or plastic intended to hold prescription spectacle lenses worn by...

  2. Frames of Reference in the Classroom

    Science.gov (United States)

    Grossman, Joshua

    2012-01-01

    The classic film "Frames of Reference" effectively illustrates concepts involved with inertial and non-inertial reference frames. In it, Donald G. Ivey and Patterson Hume use the cameras perspective to allow the viewer to see motion in reference frames translating with a constant velocity, translating while accelerating, and rotating--all with…

  3. Oversampling of wavelet frames for real dilations

    DEFF Research Database (Denmark)

    Bownik, Marcin; Lemvig, Jakob

    2012-01-01

    We generalize the Second Oversampling Theorem for wavelet frames and dual wavelet frames from the setting of integer dilations to real dilations. We also study the relationship between dilation matrix oversampling of semi-orthogonal Parseval wavelet frames and the additional shift invariance gain...

  4. Frames and generalized shift-invariant systems

    DEFF Research Database (Denmark)

    Christensen, Ole

    2004-01-01

    With motivation from the theory of Hilbert-Schmidt operators we review recent topics concerning frames in L 2 (R) and their duals. Frames are generalizations of orthonormal bases in Hilbert spaces. As for an orthonormal basis, a frame allows each element in the underlying Hilbert space to be writ...

  5. Family Reading Night

    Science.gov (United States)

    Hutchins, Darcy; Greenfeld, Marsha; Epstein, Joyce

    2007-01-01

    This book offers clear and practical guidelines to help engage families in student success. It shows families how to conduct a successful Family Reading Night at their school. Family Night themes include Scary Stories, Books We Love, Reading Olympics, Dr. Seuss, and other themes. Family reading nights invite parents to come to school with their…

  6. Reading difficulties in Albanian.

    Science.gov (United States)

    Avdyli, Rrezarta; Cuetos, Fernando

    2012-10-01

    Albanian is an Indo-European language with a shallow orthography, in which there is an absolute correspondence between graphemes and phonemes. We aimed to know reading strategies used by Albanian disabled children during word and pseudoword reading. A pool of 114 Kosovar reading disabled children matched with 150 normal readers aged 6 to 11 years old were tested. They had to read 120 stimuli varied in lexicality, frequency, and length. The results in terms of reading accuracy as well as in reading times show that both groups were affected by lexicality and length effects. In both groups, length and lexicality effects were significantly modulated by school year being greater in early grades and later diminish in length and just the opposite in lexicality. However, the reading difficulties group was less accurate and slower than the control group across all school grades. Analyses of the error patterns showed that phonological errors, when the letter replacement leading to new nonwords, are the most common error type in both groups, although as grade rises, visual errors and lexicalizations increased more in the control group than the reading difficulties group. These findings suggest that Albanian normal children use both routes (lexical and sublexical) from the beginning of reading despite of the complete regularity of Albanian, while children with reading difficulties start using sublexical reading and the lexical reading takes more time to acquire, but finally both routes are functional.

  7. Reading: United States.

    Science.gov (United States)

    Weber, Rose-Marie

    1983-01-01

    An exploration of the increasingly important role of linguistics in literacy research and instruction reviews literature on reading comprehension, written language, orthography, metalinguistics, classroom language use, reading disabilities, native tongues, nonstandard dialects, bilingual education, adult literacy, and second-language reading. (86…

  8. Dutch for Reading Knowledge

    NARCIS (Netherlands)

    van Baalen, C.; Blom, F.R.E.; Hollander, I.

    2012-01-01

    This first Dutch for Reading Knowledge book on the market promotes a high level of reading and translation competency by drawing from Dutch grammar, vocabulary and reading strategies, and providing many translation "shortcuts" and tips when tackling complex texts in Dutch. Aimed at students, researc

  9. Monster Moose Reading.

    Science.gov (United States)

    Finney, Frank

    Monster Moose (MM) Reading is a program specifically aimed at improving children's language, beginning reading, and self-concept development through the creation and utilization of student-authored reading materials which feature a series of wordless picture books about a magical moose. The MM Program is based on the following general principles…

  10. Prose reading in neglect.

    Science.gov (United States)

    Beschin, Nicoletta; Cisari, Carlo; Cubelli, Roberto; Della Sala, Sergio

    2014-02-01

    Prose reading has been shown to be a very sensitive measure of Unilateral Spatial Neglect. However, little is known about the relationship between prose reading and other measures of neglect and its severity, or between prose reading and single word reading. Thirty participants with a first stroke in the right hemisphere and clear symptoms of spatial neglect in everyday life were assessed with tests of prose reading (text in one column book-like, and in two columns magazine-like), single words reading, and a battery of 13 tests investigating neglect. Seventy percent of these participants omitted words at the beginning of the text (left end), showing Prose Reading Neglect (PRN). The participants showing PRN differed from those not showing PRN only for the overall severity of neglect, and had a lesion centred on the insula, putamen and superior temporal gyrus. Double dissociations emerged between PRN and single word reading neglect, suggesting different cognitive requirements between the two tests: parallel processing in single word reading vs. serial analysis in text reading. Notably, the pattern of neglected text varied dramatically across participants presenting with PRN, including dissociations between reading performance of one and two columns text. Prose reading proved a complex and unique task which should be directly investigated to predict the effects of unilateral neglect. The outcome of this study should also inform clinical assessment and advises given to patients and care-givers.

  11. Reading/Writing Connection.

    Science.gov (United States)

    Fernandez, Melanie

    In the past, students and teachers alike viewed reading and writing instruction as two separate entities. Reading and writing instruction was often characterized by linear and behaviorist theories and methods, with students rarely coming away from their schooling experience with confidence in and respect for their own writing. To both read and…

  12. Reading and Empathy

    Science.gov (United States)

    McCreary, John J.; Marchant, Gregory J.

    2017-01-01

    The relationship between reading and empathy was explored. Controlling for GPA and gender, reading variables were hypothesized as related to empathy; the relationship was expected to differ for males and females. For the complete sample, affective components were related to GPA but not reading. Perspective taking was related to reading…

  13. Voiced Reading and Rhythm

    Institute of Scientific and Technical Information of China (English)

    詹艳萍

    2007-01-01

    Since voiced reading is an important way in learning English,rhythm is the most critical factor that enables to read beautifully.This article illustrates the relationship between rhythm and voiced reading,the importance of rhythm,and the methods to develop the sense of rhythm.

  14. Free Reading Is UTOPIA

    Science.gov (United States)

    LeCrone, Nancy

    2010-01-01

    In high school students get tied up in extracurricular activities and have little time for pleasure reading. It is true that with rigorous academic schedules they have little time for pleasure reading. Thus began a conversation with a sophomore English teacher at the author's high school. As they were discussing the plight of free reading he was…

  15. Reference frame for Product Configuration

    DEFF Research Database (Denmark)

    Ladeby, Klaes Rohde; Oddsson, Gudmundur Valur

    2011-01-01

    on configuration systems in the shape of anecdotal reporting on the development of information systems that perhaps support the configuration task – perhaps not. Consequently, the definition of configuration has become ambiguous as different research groups defines configuration differently. This paper propose...... a reference frame for configuration that permits 1) a more precise understanding of a configuration system, 2) a understanding of how the configuration system relate to other systems, and 3) a definition of the basic concepts in configuration. The total configuration system, together with the definition...

  16. Quantum games in noninertial frame

    CERN Document Server

    Khan, Salman

    2011-01-01

    We study the influence of Unruh effect on quantum non-zero sum games. In particular, we investigate the quantum prisoners' dilemma under different conditions and show that the acceleration of the noninertial frames disturbs the symmetry of the game. It is shown that under some particular situations, the classical strategy C (cooperation) becomes the dominant strategy. Our investigation shows that any quantum strategy does no better for any player against the classical strategies. The miracle move of Eisert et al [5] is no more a superior move. We show that the dilemma like situation under every condition of the game is resolved.

  17. Gauge fields in accelerated frames

    CERN Document Server

    Lenz, F

    2008-01-01

    Quantized fields in accelerated frames (Rindler spaces) with emphasis on gauge fields are investigated. Important properties of the dynamics in Rindler spaces are shown to follow from the scale invariance of the corresponding Hamiltonians. Origin and consequences of this extraordinary property of Hamiltonians in Rindler spaces are elucidated. Characteristics of the Unruh radiation, the appearance of a photon condensate and the interaction energy of vector and scalar static charges are discussed and implications for Yang-Mills theories and QCD in Rindler spaces are indicated.

  18. Do reading and spelling share a lexicon?

    Science.gov (United States)

    Jones, Angela C; Rawson, Katherine A

    2016-05-01

    In the reading and spelling literature, an ongoing debate concerns whether reading and spelling share a single orthographic lexicon or rely upon independent lexica. Available evidence tends to support a single lexicon account over an independent lexica account, but evidence is mixed and open to alternative explanation. In the current work, we propose another, largely ignored account--separate-but-shared lexica--according to which reading and spelling have separate orthographic lexica, but information can be shared between them. We report three experiments designed to competitively evaluate these three theoretical accounts. In each experiment, participants learned new words via reading training and/or spelling training. The key manipulation concerned the amount of reading versus spelling practice a given item received. Following training, we assessed both response time and accuracy on final outcome measures of reading and spelling. According to the independent lexica account, final performance in one modality will not be influenced by the level of practice in the other modality. According to the single lexicon account, final performance will depend on the overall amount of practice regardless of modality. According to the separate-but-shared account, final performance will be influenced by the level of practice in both modalities but will benefit more from same-modality practice. Results support the separate-but-shared account, indicating that reading and spelling rely upon separate lexica, but information can be shared between them.

  19. Youth sports & public health: framing risks of mild traumatic brain injury in american football and ice hockey.

    Science.gov (United States)

    Bachynski, Kathleen E; Goldberg, Daniel S

    2014-01-01

    The framing of the risks of experiencing mild traumatic brain injury in American football and ice hockey has an enormous impact in defining the scope of the problem and the remedies that are prioritized. According to the prevailing risk frame, an acceptable level of safety can be maintained in these contact sports through the application of technology, rule changes, and laws. An alternative frame acknowledging that these sports carry significant risks would produce very different ethical, political, and social debates.

  20. Rodent-specific alternative exons are more frequent in rapidly evolving genes and in paralogs

    Directory of Open Access Journals (Sweden)

    Mironov Andrey A

    2009-06-01

    Full Text Available Abstract Background Alternative splicing is an important mechanism for generating functional and evolutionary diversity of proteins in eukaryotes. Here, we studied the frequency and functionality of recently gained, rodent-specific alternative exons. Results We projected the data about alternative splicing of mouse genes to the rat, human, and dog genomes, and identified exons conserved in the rat genome, but missing in more distant genomes. We estimated the frequency of rodent-specific exons while controlling for possible residual conservation of spurious exons. The frequency of rodent-specific exons is higher among predominantly skipped exons and exons disrupting the reading frame. Separation of all genes by the rate of sequence evolution and by gene families has demonstrated that rodent-specific cassette exons are more frequent in rapidly evolving genes and in rodent-specific paralogs. Conclusion Thus we demonstrated that recently gained exons tend to occur in fast-evolving genes, and their inclusion rate tends to be lower than that of older exons. This agrees with the theory that gain of alternative exons is one of the major mechanisms of gene evolution.

  1. Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani.

    Science.gov (United States)

    McNeil, Bonnie A; Simon, Dawn M; Zimmerly, Steven

    2014-02-01

    Group II introns are ribozymes and retroelements found in bacteria, and are thought to have been the ancestors of nuclear pre-mRNA introns. Whereas nuclear introns undergo prolific alternative splicing in some species, group II introns are not known to carry out equivalent reactions. Here we report a group II intron in the human pathogen Clostridium tetani, which undergoes four alternative splicing reactions in vivo. Together with unspliced transcript, five mRNAs are produced, each encoding a distinct surface layer protein isoform. Correct fusion of exon reading frames requires a shifted 5' splice site located 8 nt upstream of the canonical boundary motif. The shifted junction is accomplished by an altered IBS1-EBS1 pairing between the intron and 5' exon. Growth of C. tetani under a variety of conditions did not result in large changes in alternative splicing levels, raising the possibility that alternative splicing is constitutive. This work demonstrates a novel type of gene organization and regulation in bacteria, and provides an additional parallel between group II and nuclear pre-mRNA introns.

  2. What oral text reading fluency can reveal about reading comprehension

    NARCIS (Netherlands)

    Veenendaal, N.J.; Groen, M.A.; Verhoeven, L.T.W.

    2015-01-01

    Text reading fluency – the ability to read quickly, accurately and with a natural intonation – has been proposed as a predictor of reading comprehension. In the current study, we examined the role of oral text reading fluency, defined as text reading rate and text reading prosody, as a contributor t

  3. Framing Effects: Dynamics and Task Domains

    Science.gov (United States)

    Wang

    1996-11-01

    The author examines the mechanisms and dynamics of framing effects in risky choices across three distinct task domains (i.e., life-death, public property, and personal money). The choice outcomes of the problems presented in each of the three task domains had a binary structure of a sure thing vs a gamble of equal expected value; the outcomes differed in their framing conditions and the expected values, raging from 6000, 600, 60, to 6, numerically. It was hypothesized that subjects would become more risk seeking, if the sure outcome was below their aspiration level (the minimum requirement). As predicted, more subjects preferred the gamble when facing the life-death choice problems than facing the counterpart problems presented in the other two task domains. Subjects' risk preference varied categorically along the group size dimension in the life-death domain but changed more linearly over the expected value dimension in the monetary domain. Framing effects were observed in 7 of 13 pairs of problems, showing a positive frame-risk aversion and negative frame-risk seeking relationship. In addition, two types of framing effects were theoretically defined and empirically identified. A bidirectional framing effect involves a reversal in risk preference, and occurs when a decision maker's risk preference is ambiguous or weak. Four bidirectional effects were observed; in each case a majority of subjects preferred the sure outcome under a positive frame but the gamble under a negative frame. In contrast, a unidirectional framing effect refers to a preference shift due to the framing of choice outcomes: A majority of subjects preferred one choice outcome (either the sure thing or the gamble) under both framing conditions, with positive frame augmented the preference for the sure thing and negative frame augmented the preference for the gamble. These findings revealed some dynamic regularities of framing effects and posed implications for developing predictive and testable

  4. Cheetah: A high frame rate, high resolution SWIR image camera

    Science.gov (United States)

    Neys, Joel; Bentell, Jonas; O'Grady, Matt; Vermeiren, Jan; Colin, Thierry; Hooylaerts, Peter; Grietens, Bob

    2008-10-01

    A high resolution, high frame rate InGaAs based image sensor and associated camera has been developed. The sensor and the camera are capable of recording and delivering more than 1700 full 640x512pixel frames per second. The FPA utilizes a low lag CTIA current integrator in each pixel, enabling integration times shorter than one microsecond. On-chip logics allows for four different sub windows to be read out simultaneously at even higher rates. The spectral sensitivity of the FPA is situated in the SWIR range [0.9-1.7 μm] and can be further extended into the Visible and NIR range. The Cheetah camera has max 16 GB of on-board memory to store the acquired images and transfer the data over a Gigabit Ethernet connection to the PC. The camera is also equipped with a full CameralinkTM interface to directly stream the data to a frame grabber or dedicated image processing unit. The Cheetah camera is completely under software control.

  5. EFFECTS OF MESSAGE FRAMING AND EXEMPLARS ON PROMOTING ORGAN DONATION.

    Science.gov (United States)

    Chien, Yu-Hung; Chang, Wen-Te

    2015-12-01

    People in many countries are unwilling to donate organs. Drawing on previous research regarding the use of message framing and the theory of exemplification promoting intentions to donate organs, this study examined messaging strategies. This study used a 2 × 2 between-subjects factorial design to examine the joint effects of gain/loss frames and statistical/exemplar appeals on the intentions of 189 Taiwanese college students (108 women, 81 men; age range = 19-24 yr., M = 21.6, SD = 2.9) regarding organ donation. Each participant was randomly assigned to read one of four versions of an organ donation promotional message and then to complete a questionnaire. The analysis of variance showed a significant interaction between the two factors. Loss-exemplar messages elicited significantly more positive intentions toward donation than did loss-statistical messages. There was no significant difference between the statistical and exemplar appeals observed under the gain-framed condition. The practical implications of developing effective organ donation promotional materials and the limitations of this study are discussed.

  6. Magnetostrictive Alternator

    Science.gov (United States)

    Dyson, Rodger; Bruder, Geoffrey

    2013-01-01

    This innovation replaces the linear alternator presently used in Stirling engines with a continuous-gradient, impedance-matched, oscillating magnetostrictive transducer that eliminates all moving parts via compression, maintains high efficiency, costs less to manufacture, reduces mass, and eliminates the need for a bearing system. The key components of this new technology are the use of stacked magnetostrictive materials, such as Terfenol-D, under a biased magnetic and stress-induced compression, continuous-gradient impedance-matching material, coils, force-focusing metallic structure, and supports. The acoustic energy from the engine travels through an impedancematching layer that is physically connected to the magnetostrictive mass. Compression bolts keep the structure under compressive strain, allowing for the micron-scale compression of the magnetostrictive material and eliminating the need for bearings. The relatively large millimeter displacement of the pressure side of the impedance-matching material is reduced to micron motion, and undergoes stress amplification at the magnetostrictive interface. The alternating compression and expansion of the magnetostrictive material creates an alternating magnetic field that then induces an electric current in a coil that is wound around the stack. This produces electrical power from the acoustic pressure wave and, if the resonant frequency is tuned to match the engine, can replace the linear alternator that is commonly used.

  7. Growing Alternatives

    DEFF Research Database (Denmark)

    Bagger-Petersen, Mai Corlin

    2014-01-01

    From 2014, Anhui Province will pilot a reform of the residential land market in China, thus integrating rural Anhui in the national housing market. In contrast, artist and activist Ou Ning has proposed the Bishan time money currency, intending to establish an alternative economic circuit in Bishan...

  8. Alternative Treatments

    Science.gov (United States)

    ... triglyceride (fat) produced by processing coconut oil or palm kernel oil. The body breaks down caprylic acid into substances called “ketone bodies.” The theory behind Axona is that the ketone bodies derived from caprylic acid may provide an alternative energy source for brain cells that have lost ...

  9. Hunting down frame shifts: Ecological analysis of diverse functional gene sequences

    Directory of Open Access Journals (Sweden)

    Michal eStrejcek

    2015-11-01

    Full Text Available Functional gene ecological analyses using amplicon sequencing can be challenging as translated sequences are often burdened with shifted reading frames. The aim of this work was to evaluate several bioinformatics tools designed to correct errors which arise during sequencing in an effort to reduce the number of frame-shifts (FS. Genes encoding for alpha subunits of biphenyl (bphA and benzoate (benA dioxygenases were used as model sequences. FrameBot, a FS correction tool, was able to reduce the number of detected FS to zero. However, up to 43.1% of sequences were discarded by FrameBot as non-specific targets. Therefore, we proposed a de novo mode of FrameBot for FS correction, which works on a similar basis as common chimera identifying platforms and is not dependent on reference sequences. By nature of FrameBot de novo design, it is crucial to provide it with data as error free as possible. We tested the ability of several publicly available correction tools to decrease the number of errors in the data sets. The combination of Maximum Expected Error (MEE filtering and single linkage pre-clustering (SLP proved the most efficient read procession. Applying FrameBot de novo on the processed data enabled analysis of BphA sequences with minimal losses of potentially functional sequences not homologous to those previously known. This experiment also demonstrated the extensive diversity of dioxygenases in soil. A script which performs FrameBot de novo is presented in the supplementary material to the study and the tool was implemented into FunGene Pipeline available at http://fungene.cme.msu.edu/FunGenePipeline/ and https://github.com/rdpstaff/Framebot.

  10. A Formal Description of Problem Frames

    Directory of Open Access Journals (Sweden)

    Souleymane KOUSSOUBE

    2014-03-01

    Full Text Available Michael Jackson defines a Problem Frame as a mean to describe and classify software development problems. The initial description of problem Frames is essentially graphical. A weakness of this proposal is the lack of formal specification allowing efficient reasoning tools. This paper deals with Problem Frames’ formal specification with Description Logics. We first propose a formal terminology of Problem Frames leading to the specification of a Problem Frames’ TBOX and a specific problem’s ABOX. The Description Logics inference tools can then be used to decompose multi frame problems or to fix a particular problem into a Problem Frame.

  11. Manufacturing of neutron reflector frame for JMTR

    OpenAIRE

    2010-01-01

    Beryllium has been used as the neutron reflector in the Japan Materials Testing Reactor (JMTR). A beryllium frame is arranged in the JMTR core and the frame consists of 3 sections (North, East and West). Each section has 7 stories of the beryllium blocks. Each block is connected by the aluminum joints. The capsule or the beryllium plug is located in the inside of the beryllium frame. The first criticality achieved in 1968 and the frame has been replaced 6 times and now the 7th frame is being ...

  12. Amblyopic reading is crowded.

    Science.gov (United States)

    Levi, Dennis M; Song, Shuang; Pelli, Denis G

    2007-10-26

    We measure acuity, crowding, and reading in amblyopic observers to answer four questions. (1) Is reading with the amblyopic eye impaired because of larger required letter size (i.e., worse acuity) or larger required spacing (i.e., worse crowding)? The size or spacing required to read at top speed is called "critical". For each eye of seven amblyopic observers and the preferred eyes of two normal observers, we measure reading rate as a function of the center-to-center spacing of the letters in central and peripheral vision. From these results, we estimate the critical spacing for reading. We also measured traditional acuity for an isolated letter and the critical spacing for identifying a letter among other letters, which is the classic measure of crowding. For both normals and amblyopes, in both central and peripheral vision, we find that the critical spacing for reading equals the critical spacing for crowding. The identical critical spacings, and very different critical sizes, show that crowding, not acuity, limits reading. (2) Does amblyopia affect peripheral reading? No. We find that amblyopes read normally with their amblyopic eye except that abnormal crowding in the fovea prevents them from reading fine print. (3) Is the normal periphery a good model for the amblyopic fovea? No. Reading centrally, the amblyopic eye has an abnormally large critical spacing but reads all larger spacings at normal rates. This is unlike the normal periphery, in which both critical spacing and maximum reading rate are severely impaired relative to the normal fovea. (4) Can the uncrowded-span theory of reading rate explain amblyopic reading? Yes. The case of amblyopia shows that crowding limits reading solely by determining the uncrowded span: the number of characters that are not crowded. Characters are uncrowded if and only if their spacing is more than critical. The text spacing may be uniform, but the observer's critical spacing increases with distance from fixation, so the

  13. The Relations between Discrete Frames and Continuous Frames with Respect to (N, µ)

    Institute of Scientific and Technical Information of China (English)

    LIU Chun-tai

    2013-01-01

    The discrete frame and the continuous frame in a Hilbert space are discussed. By the tool excess of a sequence, a sufficient and necessary condition is presented under which a discrete frame is equivalent to a continuous frame with respect to the whole natural numbers with a positive Borel measure. And some examples are given to illuminate the condition.

  14. Frame ambiguity in policy controversies: critical frame analysis of migrant integration policies in Antwerp and Rotterdam

    NARCIS (Netherlands)

    R. Dekker (Rianne)

    2016-01-01

    textabstractPolicy frames are understood as the outcome of a policy process in which multiple frames are contesting, but where one frame prevails and characterizes policies. Policy frames are therefore perceived and studied as coherent interpretations of a policy issue containing a problem definitio

  15. Message framing in social networking sites.

    Science.gov (United States)

    Kao, Danny Tengti; Chuang, Shih-Chieh; Wang, Sui-Min; Zhang, Lei

    2013-10-01

    Online social networking sites represent significant new opportunities for Internet advertisers. However, results based on the real world cannot be generalized to all virtual worlds. In this research, the moderating effects of need for cognition (NFC) and knowledge were applied to examine the impact of message framing on attitudes toward social networking sites. A total of 216 undergraduates participated in the study. Results reveal that for social networking sites, while high-NFC individuals form more favorable attitudes toward negatively framed messages than positively framed messages, low-NFC individuals form more favorable attitudes toward positively framed messages than negatively framed messages. In addition, low-knowledge individuals demonstrate more favorable attitudes toward negatively framed messages than positively framed messages; however, the framing effect does not differentially affect the attitudes of high-knowledge individuals. Furthermore, the framing effect does not differentially affect the attitudes of high-NFC individuals with high knowledge. In contrast, low-NFC individuals with low knowledge hold more favorable attitudes toward positively framed messages than negatively framed messages.

  16. Analisis Framing dalam Riset Public Relations

    Directory of Open Access Journals (Sweden)

    NARAYANA MAHENDRA PRASTYA

    2016-12-01

    Full Text Available This paper aims to give description about how to use frame analysis in Public Relations (PR research. The author use two framing models: Entman and Pan & Kosicki. The object is organization official statement about particular issue. Frame analysis method rarely used in Public Relations research. This methods commonly use in journalism study, to analyse the news in media. Meanwhile, the key word of framing is the social construction of reality. Organization can make social construction of realty in their official statement. In acacemic term, frame analysis in PR research is useful to know how organization positioned themselves in particular situation. Other benefit is use to evaluat whether the organization frame is conformable with the public opinion or agenda setting media or not. In practical term, frame analysis give benefit for PR practitioner to create the message that can be undserstood by public, also give positive image for organization.

  17. "Think" versus "feel" framing effects in persuasion.

    Science.gov (United States)

    Mayer, Nicole D; Tormala, Zakary L

    2010-04-01

    Three studies explored think ("I think . . . ") versus feel ("I feel . . . ") message framing effects on persuasion.The authors propose a matching hypothesis, suggesting that think framing will be more persuasive when the target attitude or message recipient is cognitively oriented, whereas feel framing will be more persuasive when the target attitude or message recipient is affectively oriented. Study 1 presented cognitively and affectively oriented individuals with a think- or feel-framed message. Study 2 primed cognitive or affective orientation and then presented a think- or feel-framed message. Study 3 presented male and female participants with an advertisement containing think- or feel-framed arguments. Results indicated that think (feel) framing was more persuasive when the target attitude or recipient was cognitively (affectively) oriented. Moreover, Study 2 demonstrated that this matching effect was mediated by processing fluency. Theoretical and practical implications are discussed.

  18. Nonlinear pushover analysis of infilled concrete frames

    Institute of Scientific and Technical Information of China (English)

    Chao Hsun Huang; Yungting Alex Tuan; Ruo Yun Hsu

    2006-01-01

    Six reinforced concrete frames with or without masonry infills were constructed and tested under horizontal cyclic loads. All six frames had identical details in which the transverse reinforcement in columns was provided by rectangular hoops that did not meet current ACI specifications for ductile frames. For comparison purposes, the columns in three of these frames were jacketed by carbon-fiber-reinforced-polymer (CFRP) sheets to avoid possible shear failure. A nonlinear pushover analysis, in which the force-deformation relationships of individual elements were developed based on ACI 318, FEMA 356,and Chen's model, was carried out for these frames and compared to test results. Both the failure mechanisms and impact of infills on the behaviors of these frames were examined in the study. Conclusions from the present analysis provide structural engineers with valuable information for evaluation and design of infilled concrete frame building structures.

  19. Framing: la perspectiva de las noticias

    Directory of Open Access Journals (Sweden)

    Aruguete, Natalia

    2011-01-01

    Full Text Available [es] Este trabajo consiste en una revisión teórica de la Teoría del Framing o Teoría del Encuadre. A lo largo del texto se desarrollan los orígenes de esta corriente, las definiciones propuestas para el término frame o framing por diversos autores, los tipos de frames existentes, las dimensiones utilizadas para su estudio empírico y los abordajes metodológicos para su identificación en la información mediática. [en] This work is a theoretical review of the Framing Theory. Throughout the text develops the origins of this perspective, the definitions proposed for the concept frame by different authors, the existing types of frames, the dimensions used for empirical research and methodological approaches for identification of the information media

  20. Good things don't come easy (to mind): explaining framing effects in judgments of truth.

    Science.gov (United States)

    Hilbig, Benjamin E

    2012-01-01

    Recently, the general phenomenon of a positive-negative-asymmetry was extended to judgments of truth. That is, negatively framed statements were shown to receive substantially higher truth ratings than formally equivalent statements framed positively. However, the cognitive mechanisms underlying this effect are unknown, so far. In the current work, two potential accounts are introduced and tested against each other in three experiments: On the one hand, negative framing may induce increased elaboration and thereby persuasion. Alternatively, negative framing could yield faster retrieval or generation of evidence and thus influence subjective veracity via experiential fluency. Two experiments drawing on response latencies and one manipulating the delay between information acquisition and judgment provide support for the fluency-based account. Overall, results replicate and extend the negatively-biased framing effect in truth judgments and show that processing fluency may account for it.

  1. Relaxing Tight Frame Condition in Parallel Proximal Methods for Signal Restoration

    CERN Document Server

    Pustelnik, Nelly; Chaux, Caroline

    2011-01-01

    A fruitful approach for solving signal deconvolution problems consists of resorting to a frame-based convex variational formulation. In this context, parallel proximal algorithms and related alternating direction methods of multipliers have become popular optimization techniques to approximate iteratively the desired solution. Until now, in most of these methods, either Lipschitz differentiability properties or tight frame representations were assumed. In this paper, it is shown that it is possible to relax these assumptions by considering a class of non necessarily tight frame representations, thus offering the possibility of addressing a broader class of signal restoration problems. In particular, it is possible to use non necessarily maximally decimated filter banks with perfect reconstruction, which are common tools in digital signal processing. The proposed approach allows us to solve both frame analysis and frame synthesis problems for various noise distributions. In our simulations, it is applied to th...

  2. Interaction and framing in the performance “Insideout” by Sasha Waltz

    DEFF Research Database (Denmark)

    Thygesen, Mads

    2010-01-01

    This paper examines how Waltz’ performance "Insideout" (2003) on the one hand iterates some relatively stable contextual frames of “sameness” (its mise en scène), but on the other, more radically plays with different and alternating discursive framings or contexts, e.g. “this is theatre” or “this...... is a social or political situation”, in order to create a space of interaction between actors and spectators. This challenges a more traditional notion of the theatrical frame (Goffmann): given that the dividing line between performer and audience is blurred, the audience becomes involved in the production...

  3. Fingerprinting with Equiangular Tight Frames

    CERN Document Server

    Mixon, Dustin G; Kiyavash, Negar; Fickus, Matthew

    2011-01-01

    Digital fingerprinting is a framework for marking media files, such as images, music, or movies, with user-specific signatures to deter illegal distribution. Multiple users can collude to produce a forgery that can potentially overcome a fingerprinting system. This paper proposes an equiangular tight frame fingerprint design which is robust to such collusion attacks. We motivate this design by considering digital fingerprinting in terms of compressed sensing. The attack is modeled as linear averaging of multiple marked copies before adding a Gaussian noise vector. The content owner can then determine guilt by exploiting correlation between each user's fingerprint and the forged copy. The worst-case error probability of this detection scheme is analyzed and bounded. Simulation results demonstrate the average-case performance is similar to the performance of orthogonal and simplex fingerprint designs, while accommodating several times as many users.

  4. The framing of scientific domains

    DEFF Research Database (Denmark)

    Dam Christensen, Hans

    2014-01-01

    Purpose: By using the UNISIST models this article argues for the necessity of domain analysis in order to qualify scientific information seeking. The models better understanding of communication processes in a scientific domain and embraces the point that domains are always both unstable over time...... as according to the agents that are charting them. As such, power in a Foucauldian sense is unavoidable in outlining a domain. Originality/value 1. The UNISIST models are applied to the domain of art history; and 2. the article discusses the instability of a scientific domain as well as, at the same time......, the significance of framing a domain; an implication which is often neglected in scientific information seeking....

  5. Exploring the Relationship between Adolescent's Reading Skills, Reading Motivation and Reading Habits

    Science.gov (United States)

    McGeown, Sarah P.; Duncan, Lynne G.; Griffiths, Yvonne M.; Stothard, Sue E.

    2015-01-01

    The present study examines the extent to which adolescents' reading affect (reading motivation) and behaviour (reading habits) predict different components of reading (word reading, comprehension, summarisation and text reading speed) and also adds to the limited research examining group differences (gender, age, ability) in adolescents' reading…

  6. Alternative Design of Postgraduate English Writing Course

    Institute of Scientific and Technical Information of China (English)

    王芬

    2008-01-01

    This paper presents an alternative design of postgraduate English writing course to meet the requirement of the current postgraduate English teaching reform.The author believes that efficiently-organized writing activities based on practical needs could refresh teaching frame- work and consequently assist postgraduates in English writing.

  7. Translational Maintenance of Frame: Mutants of Saccharomyces Cerevisiae with Altered -1 Ribosomal Frameshifting Efficiences

    OpenAIRE

    Dinman, J D; Wickner, R B

    1994-01-01

    A special site on the (+) strand of the L-A dsRNA virus induces about 2% of ribosomes translating the gag open reading frame to execute a -1 frameshift and thus produce the viral gag-pol fusion protein. Using constructs in which a -1 ribosomal frameshift at this site was necessary for expression of lacZ we isolated chromosomal mutants in which the efficiency of frameshifting was increased. These mutants comprise eight genes, named mof (maintenance of frame). The mof1-1, mof2-1, mof4-1, mof5-1...

  8. Framing effects: behavioral dynamics and neural basis.

    Science.gov (United States)

    Zheng, Hongming; Wang, X T; Zhu, Liqi

    2010-09-01

    This study examined the neural basis of framing effects using life-death decision problems framed either positively in terms of lives saved or negatively in terms of lives lost in large group and small group contexts. Using functional MRI we found differential brain activations to the verbal and social cues embedded in the choice problems. In large group contexts, framing effects were significant where participants were more risk seeking under the negative (loss) framing than under the positive (gain) framing. This behavioral difference in risk preference was mainly regulated by the activation in the right inferior frontal gyrus, including the homologue of the Broca's area. In contrast, framing effects diminished in small group contexts while the insula and parietal lobe in the right hemisphere were distinctively activated, suggesting an important role of emotion in switching choice preference from an indecisive mode to a more consistent risk-taking inclination, governed by a kith-and-kin decision rationality.

  9. Do framing effects reveal irrational choice?

    Science.gov (United States)

    Mandel, David R

    2014-06-01

    Framing effects have long been viewed as compelling evidence of irrationality in human decision making, yet that view rests on the questionable assumption that numeric quantifiers used to convey the expected values of choice options are uniformly interpreted as exact values. Two experiments show that when the exactness of such quantifiers is made explicit by the experimenter, framing effects vanish. However, when the same quantifiers are given a lower bound (at least) meaning, the typical framing effect is found. A 3rd experiment confirmed that most people spontaneously interpret the quantifiers in standard framing tests as lower bounded and that their interpretations strongly moderate the framing effect. Notably, in each experiment, a significant majority of participants made rational choices, either choosing the option that maximized expected value (i.e., lives saved) or choosing consistently across frames when the options were of equal expected value.

  10. The electromagnetic field in accelerated frames

    CERN Document Server

    Maluf, J W

    2011-01-01

    We develop a geometrical framework that allows to obtain the electromagnetic field quantities in accelerated frames. The frame of arbitrary accelerated observers in space-time is defined by a suitable set of tetrad fields, whose timelike components are adapted to the worldlines of a field of observers. We consider the Faraday tensor and Maxwell's equations as abstract tensor quantities in space-time, and make use of tetrad fields to project the electromagnetic field quantities in the accelerated frames. As an application, plane and spherical electromagnetic waves are projected in linearly accelerated frames in Minkowski space-time. We show that the amplitude, frequency and the wave vector of the plane wave in the accelerated frame vary with time, while the light speed remains constant. We also obtain the variation of the Poynting vector with time in the accelerated frame.

  11. Reading pictures for story comprehension requires mental imagery skills

    NARCIS (Netherlands)

    Boerma, I.E.; Mol, S.E.; Jolles, J.

    2016-01-01

    We examined the role of mental imagery skills on story comprehension in 150 fifth graders (10- to 12-year-olds), when reading a narrative book chapter with alternating words and pictures (i.e., text blocks were alternated by one- or two-page picture spreads). A parallel group design was used, in whi

  12. Improve your reading

    CERN Document Server

    Fry, Ron

    2012-01-01

    Help your students discover the practical solution to their reading frustrations, with Improve Your Reading. Written by bestselling author and education advocate Ron Fry, this book avoids gimmicks and tricks in favor of proven strategies that will help your students better retain and comprehend what they've read in any textbook, in any course, at any academic level. Endlessly adaptable to each student's individual learning needs, the text focuses on fundamental skills students can carry beyond the classroom.

  13. The Heroic Framing of US Foreign Policy

    OpenAIRE

    2010-01-01

    This dissertation concerns the US presidential use of heroic framing in connection with foreign policy. I identify "heroic framing" as speech which describes policies in terms evoking the heroic narrative, either explicitly or implicitly through references to heroic characters. The technique of heroic framing both encourages audiences to view a situation in terms of stark moral absolutes and normalizes casualties and violence as an aspect of these heroic conflicts. This rhetorical technique t...

  14. Reading disorders and dyslexia

    Science.gov (United States)

    Hulme, Charles; Snowling, Margaret J.

    2016-01-01

    Purpose of review We review current knowledge about the nature of reading development and disorders, distinguishing between the processes involved in learning to decode print, and the processes involved in reading comprehension. Recent findings Children with decoding difficulties/dyslexia experience deficits in phoneme awareness, letter-sound knowledge and rapid automatized naming in the preschool years and beyond. These phonological/language difficulties appear to be proximal causes of the problems in learning to decode print in dyslexia. We review data from a prospective study of children at high risk of dyslexia to show that being at family risk of dyslexia is a primary risk factor for poor reading and children with persistent language difficulties at school entry are more likely to develop reading problems. Early oral language difficulties are strong predictors of later difficulties in reading comprehension. Summary There are two distinct forms of reading disorder in children: dyslexia (a difficulty in learning to translate print into speech) and reading comprehension impairment. Both forms of reading problem appear to be predominantly caused by deficits in underlying oral language skills. Implications for screening and for the delivery of robust interventions for language and reading are discussed. PMID:27496059

  15. On Reading Test

    Institute of Scientific and Technical Information of China (English)

    孙健

    2005-01-01

    There has been a long discussion over the construct validity of reading tests. In china's reading tests, multiple choice is the main test method in view of the4 long controversy over the validity of multiple choice, construct validation is called for to empirically test the hypothesized relationships between test scores and abilities. The national CET committee conducted a comprehensive validation study. As part of the project, the specialists studied the reading comprehension test's validity by qualitative means, namely "introspective verbal reports". The analysis revealed that an overwhelming majority of the questions items were handled through "expected reading operations".

  16. Journalism Curiosity and Story Telling Frame

    DEFF Research Database (Denmark)

    Grunwald, Ebbe; Rupar, Verica

    2009-01-01

    This comparative study of journalism practices in Australia and Denmark explores the interplay between two concepts relevant for journalism's meaning-making activity: a curiosity seen as an action meant to close an information gap, and a story telling frame seen as a form of structuring information...... the epistemological and organisational dimension of frames relates to the process of  meaning-making. We suggest refining the concept of frame in journalism studies by making a distinction between a frame (an epistemological category) and an angle (a textual organisation category). Our investigation shows...... that this distinction better serves the analysis and understanding of the mechanisms behind journalism in comparative contexts. Udgivelsesdato: December...

  17. Pyramidal space frame and associated methods

    Science.gov (United States)

    Clark, Ryan Michael; White, David; Farr, Jr, Adrian Lawrence

    2016-07-19

    A space frame having a high torsional strength comprising a first square bipyramid and two planar structures extending outward from an apex of the first square bipyramid to form a "V" shape is disclosed. Some embodiments comprise a plurality of edge-sharing square bipyramids configured linearly, where the two planar structures contact apexes of all the square bipyramids. A plurality of bridging struts, apex struts, corner struts and optional internal bracing struts increase the strength and rigidity of the space frame. In an embodiment, the space frame supports a solar reflector, such as a parabolic solar reflector. Methods of fabricating and using the space frames are also disclosed.

  18. Assisted Reading with Digital Audiobooks for Students with Reading Disabilities

    Science.gov (United States)

    Esteves, Kelli J.; Whitten, Elizabeth

    2011-01-01

    The goal of this study was to compare the efficacy of assisted reading with digital audiobooks with the traditional practice of sustained silent reading (SSR) in terms of reading fluency and reading attitude with upper elementary students with reading disabilities. Treatment group participants selected authentic children's literature and engaged…

  19. How to Develop Reading Strategies in Extensive Reading

    Institute of Scientific and Technical Information of China (English)

    陈腊梅

    2016-01-01

    Extensive reading demands readers to read in quantity for general, overall meaning, and for information. Learners are supposed to know how to choose appropriate reading tactics in accordance with different reading purposes. This paper mainly proposes some effective strategies to develop learners' reading skills and competency.

  20. Reading Strategy: Tackling Reading through Topic and Main Ideas

    Science.gov (United States)

    Naidu, Bharathi; Briewin, Marshal; Embi, Mohamed Amin

    2013-01-01

    Reading comprehension is one of the four skills essential in learning English. In a reading class, students tend to read all the information provided in reading materials, but how much do they actually retain? This study explores whether learners use identification of the topic and main ideas as a reading strategy to assist in reading…

  1. Temporal Consequences, Message Framing, and Consideration of Future Consequences: Persuasion Effects on Adult Fruit Intake Intention and Resolve.

    Science.gov (United States)

    de Bruijn, Gert-Jan; Budding, Jeen

    2016-08-01

    Message framing is a persuasive strategy that has seen mixed evidence for promoting fruit intake intentions, potentially because framed messages for fruit intake have not (a) explicitly compared short-term consequences versus long-term consequences, (b) considered individual-level differences in time perspective, and (c) used alternative measures of fruit intake intentions. In the present online study, the effects of persuasive messages created from temporal context (short term vs. long term) and message frame (gain framed vs. loss framed) were investigated on fruit intake intentions and resolve among a sample of Dutch adults who were categorized as either present oriented or future oriented. For intention and resolve, results showed a significant Type of Frame × Type of Temporal Context interaction, such that gain-framed messages were more persuasive when combined with long-term consequences and loss-framed messages were more persuasive when combined with short-term consequences. The effect sizes for these differences were similar for resolve and intention, but only differences for intentions were significant. No other effects were found. These results demonstrate that message framing theory may usefully consider the inclusion of temporal context of outcomes and alternative motivation measures to maximize their persuasive effects.

  2. Alternative splicing of testis-specific lactate dehydrogenase C gene in mammals and pigeon.

    Science.gov (United States)

    Huang, Lin; Lin, Yaqiu; Jin, Suyu; Liu, Wei; Xu, Yaou; Zheng, Yucai

    2012-04-01

    The objective of the present study was to confirm the widespread existence of alternative splicing of lactate dehydrogenase c (ldhc) gene in mammals. RT-PCR was employed to amplify cDNAs of ldhc from testes of mammals including pig, dog, rabbit, cat, rat, and mouse, as well as pigeon. Two to six kinds of splice variants of ldhc were observed in the seven species as a result of deletion of one or more exons or insertion of partial sequence of an intron in the mature mRNA. The deleted exons occur mostly in exons 5, 4, 6, and 3. The insertion of a partial sequence of introns, which resulted in an abnormal stop codon in the inserted intron sequence, was observed only in dog and rat. The deletion of exons also resulted in a reading frame shift and formation of a stop codon in some variants. No alternative splicing was observed for ldha and ldhb genes in testis of yak. Native polyacrylamide gel electrophoresis and Western blot analysis revealed no obvious LDH-C4 activity derived from expressed ldhc variants. Our results demonstrated the widespread and unique existence of alternative splicing of ldhc genes in mammals.

  3. Recovering Innocence: Growing Up Reading.

    Science.gov (United States)

    Spencer, Patricia Sylvester

    1991-01-01

    Offers a plan for changing the perception among teenagers that reading for pleasure is an activity of their childhood past. Suggests an elective Reading Workshop that allows students to share favorite books and authors, to read aloud and listen to others read aloud, and to discuss their reading processes and decisions. (PRA)

  4. Alternative Energies

    Energy Technology Data Exchange (ETDEWEB)

    Planting, A.; De saint Jacob, Y.; Verwijs, H.; Belin, H.; Preesman, L.

    2009-03-15

    In two articles, one interview and one column attention is paid to alternative energies. The article 'A new light on saving energy' discusses the option to save energy by modernising lighting systems in urban areas. The column 'View from Paris' focuses on investment decisions in France with regard to renewable energy and energy savings. The article 'Europe turns a blind eye to big battery' discusses developments in batteries to store energy. The interview concerns fuel cell expert and formerly President of UTC Power Jan van Dokkum. The last article gives a brief overview of the European Energy Research Alliance (EERA) and the challenges this alliance will have to face with regard to climate change and energy security.

  5. HMM-FRAME: accurate protein domain classification for metagenomic sequences containing frameshift errors

    Directory of Open Access Journals (Sweden)

    Sun Yanni

    2011-05-01

    Full Text Available Abstract Background Protein domain classification is an important step in metagenomic annotation. The state-of-the-art method for protein domain classification is profile HMM-based alignment. However, the relatively high rates of insertions and deletions in homopolymer regions of pyrosequencing reads create frameshifts, causing conventional profile HMM alignment tools to generate alignments with marginal scores. This makes error-containing gene fragments unclassifiable with conventional tools. Thus, there is a need for an accurate domain classification tool that can detect and correct sequencing errors. Results We introduce HMM-FRAME, a protein domain classification tool based on an augmented Viterbi algorithm that can incorporate error models from different sequencing platforms. HMM-FRAME corrects sequencing errors and classifies putative gene fragments into domain families. It achieved high error detection sensitivity and specificity in a data set with annotated errors. We applied HMM-FRAME in Targeted Metagenomics and a published metagenomic data set. The results showed that our tool can correct frameshifts in error-containing sequences, generate much longer alignments with significantly smaller E-values, and classify more sequences into their native families. Conclusions HMM-FRAME provides a complementary protein domain classification tool to conventional profile HMM-based methods for data sets containing frameshifts. Its current implementation is best used for small-scale metagenomic data sets. The source code of HMM-FRAME can be downloaded at http://www.cse.msu.edu/~zhangy72/hmmframe/ and at https://sourceforge.net/projects/hmm-frame/.

  6. The effect of message framing on breast self-examination attitudes, intentions, and behavior.

    Science.gov (United States)

    Meyerowitz, B E; Chaiken, S

    1987-03-01

    In this study we tested the framing hypothesis that a pamphlet stressing the negative consequences of not performing breast self-examination (BSE) would be more persuasive than a pamphlet emphasizing BSE's positive consequences. College-aged female subjects were exposed to a loss-frame pamphlet, a gain-frame pamphlet, or a no-arguments pamphlet, or they received no pamphlet describing the importance of and the techniques for performing BSE. Attitudes toward BSE and intentions to perform BSE were assessed immediately after this intervention and again 4 months later. The follow-up also assessed subjects' postexperimental BSE behavior. Consistent with predictions, subjects who read a pamphlet with arguments framed in loss language manifested more positive BSE attitudes, intentions, and behaviors than did subjects in the other three conditions. The greater impact of the loss pamphlet could not be attributed to greater fear arousal, better memory for pamphlet content, greater perceived susceptibility to breast cancer, or stronger beliefs in BSE's efficacy on the part of the loss subjects. Only measures of perceived self-efficacy in performing BSE were differentially affected by the framing manipulation, with loss subjects reporting the greatest levels of self-confidence. The results are discussed in terms of prospect theory's framing postulate and a simpler negativity-bias conceptualization, and underlying mechanisms such as differential salience and vividness are considered. Clinical implications of the findings are also explored.

  7. Reading Authentic Texts

    DEFF Research Database (Denmark)

    Balling, Laura Winther

    2013-01-01

    Most research on cognates has focused on words presented in isolation that are easily defined as cognate between L1 and L2. In contrast, this study investigates what counts as cognate in authentic texts and how such cognates are read. Participants with L1 Danish read news articles in their highly...

  8. Teaching Reading in Homeschool.

    Science.gov (United States)

    Yambo, Idalia

    This paper discusses the home-schooling trend and identifies reading instructional methods used by home-schooling parents. Interviews were conducted with 5 home-schooling families of children ranging in age from 1 to 14 years. Parents reported that they began reading instruction with their child at about age 5 and agreed that instruction in…

  9. Online Reading Test Evaluation

    Institute of Scientific and Technical Information of China (English)

    雷鸣

    2011-01-01

    Language test has been used as a scientific assessment tool in providing valuable information for teaching and learning. In fact,lots of online reading tests are not designed with validity. This paper analyzes those online reading tests from the aspects o

  10. Assistive Technologies for Reading

    Science.gov (United States)

    Ruffin, Tiece M.

    2012-01-01

    Twenty-first century teachers working with diverse readers are often faced with the question of how to integrate technology in reading instruction that meets the needs of the techno-generation. Are today's teachers equipped with the knowledge of how to effectively use Assistive Technologies (AT) for reading? This position paper discusses AT for…

  11. Reading and Perestroika.

    Science.gov (United States)

    Plotnikov, Sergei N.

    1992-01-01

    Presents a short historical and sociological analysis of reading in the Soviet Union from the beginning of the twentieth century to perestroika. Discusses some sociocultural problems associated with reading, including the prevailing social, economic and political crises in all spheres of life, particularly the cultural. (RS)

  12. Reading Where It Counts

    Science.gov (United States)

    Miller, Harry

    2014-01-01

    In this article, teachers are reminded that their content subject areas require acquainting children with special words or symbols related to that subject area (e.g. mathematics or social studies). Because children can read well does not mean they would be understanding of any special reading skill required in a content subject area; that the…

  13. Lippincott Basic Reading Program.

    Science.gov (United States)

    Monterey Peninsula Unified School District, Monterey, CA.

    This program, included in "Effective Reading Programs...," serves 459 students in grades 1-3 at 15 elementary schools. The program employs a diagnostic-prescriptive approach to instruction in a nongraded setting through the use of the Lippincott Basic Reading program. When a child enters the program, he is introduced to a decoding…

  14. Uninterrupted Sustained Silent Reading.

    Science.gov (United States)

    Meyers, Rick

    A study investigated the effect Sustained Silent Reading (SSR) has had on literacy at Estancia High School in California which recently implemented an SSR program. It also examined the role SSR has on language development, comprehension, vocabulary, student attitudes, and its corollary consequence on the development of reading habits. A survey of…

  15. Cerebral Laterality and Reading.

    Science.gov (United States)

    Mackworth, Jane F.

    Recent research has confirmed that hemispheric patterns of dominance are related to reading skills. Reading is more complex than speech because it includes a visuo-spatial element. In the great majority of people, the left hemisphere deals with speech and sequencing skills. Visual matching of printed words requires the spatial skills of the right…

  16. Anything but Reading

    Science.gov (United States)

    Krashen, Stephen

    2009-01-01

    Both the popular media and professional literature are filled with suggestions on how to improve reading, but the one approach that always works is rarely mentioned: provide readers with a supply of interesting and comprehensible books. Instead, people are given advice that is dead wrong as a means of improving reading (e.g., roller skating and…

  17. Toddler Reading Time

    Science.gov (United States)

    ... Your 1- to 2-Year-Old Toddler Reading Time KidsHealth > For Parents > Toddler Reading Time A A A What's in this article? Reasons ... Kids make big leaps in vocabulary during this time, and learn about letters, shapes, colors, weather, animals, ...

  18. Books for Summer Reading.

    Science.gov (United States)

    Phi Delta Kappan, 1996

    1996-01-01

    Suggests several novels for educators' summer reading enjoyment, including classics by Robert Pirsig, Robertson Davies, John Steinbeck, Albert Camus, and Charles Dickens. Educators might also read Alex Kotlowitz's "There Are No Children Here" (Doubleday, 1991) and Sharon Quint's "Schooling Homeless Children" (Teachers College Press, 1994) to gain…

  19. LINGUISTICS AND JAPANESE READING.

    Science.gov (United States)

    CROWLEY, DALE P.

    THE PRINCIPLES OF STRUCTURAL LINGUISTICS, THE DEVELOPMENT OF JAPANESE ORTHOGRAPHY, AND THE PSYCHOLOGY OF LEARNING ARE USED AS A BASIS FOR DEVELOPMENT OF A LINGUISTICALLY ORIENTED COURSE IN JAPANESE READING. THE FIRST PART OF THE TEXT IS DEVOTED TO THE RELATION BETWEEN READING AND LINGUISTICS. THE SECOND PART GIVES BACKGROUND MATERIAL ON JAPANESE…

  20. Reading Patterns Changing

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    Modern life is changing the way people read April 23 was the 16th World Book and Copyright Day,also known as the World Book Day.Reading-related problems have once again attracted people’s attention.Today,living a life with an increasingly rapid pace,most people are

  1. Little Herder Reading Series.

    Science.gov (United States)

    Bureau of Indian Affairs (Dept. of Interior), Washington, DC.

    The Little Herder Reading Series is comprised of 4 volumes based on the life of a Navajo Indian girl. The books are written in English blank verse and describe many facets of Indian life. The volumes contain illustrations by Hoke Denetsosie which give a pictorial representation of the printed verse. The reading level is for the middle and upper…

  2. Framing the Genetics Curriculum for Social Justice: An Experimental Exploration of How the Biology Curriculum Influences Beliefs about Racial Difference

    Science.gov (United States)

    Donovan, Brian M.

    2016-01-01

    This field experiment manipulated the racial framing of a reading on human genetic disease to explore whether racial terminology in the biology curriculum affects how adolescents explain and respond to the racial achievement gap in American education. Carried out in a public high school in the San Francisco Bay Area, students recruited for the…

  3. EXTENSIVE READING IN CHINA

    Institute of Scientific and Technical Information of China (English)

    1996-01-01

    IntroductionMost teachers and students in China are quite familiar with the term‘extensive reading’,but how itshould be taught still remains a problem.This paper covers the aims of extensive reading and the methodsand materials used in the course.Then some practical suggestions will be given to make the course moreinteresting and efficient.According to Dzao(1990).extensive reading is‘the course where other reading skills-speed,predictionand making inference-can be developed,’and‘where there is practice in geting the gist,in summarisingmain ideas,in understanding the author’s purpose and theme...’.So the aims of this course are todevelop general reading skills,the ability,to read quickly and to grasp the main ideas of the text.Toachieve these,students must enlarge their vocabulary,so this is also regarded as one of the aims.

  4. Supporting Product Line Evolution with Framed Aspects

    DEFF Research Database (Denmark)

    Loughran, Neil; Rashid, Awais; Zhang, Weishan

    2004-01-01

    This paper discusses how evolution in software product lines can be supported using framed aspects: a combination of aspect-oriented programming and frame technology. Product line architectures and assets are subject to maintenance and evolution throughout their lifetime due to the emergence of n...

  5. High frame rate synthetic aperture duplex imaging

    DEFF Research Database (Denmark)

    Stuart, Matthias Bo; Tomov, Borislav Gueorguiev; Pihl, Michael Johannes

    2013-01-01

    aperture flow imaging as demonstrated in this paper. Synthetic aperture, directional beamforming, and cross-correlation are used to produce B-mode and vector velocity images at high frame rates. The frame rate equals the effective pulse repetition frequency of each imaging mode. Emissions for making the B...

  6. Teaching the Dynamics of Framing Competitions

    Science.gov (United States)

    Rinke, Eike Mark

    2012-01-01

    Framing theory is one of the most thriving and complex fields of communication theory, and as such it has grown to be an integral part of many political communication, public opinion, and communication theory courses. Part of the complexity stems from scholars' efforts to develop accounts of framing processes that are closer to the "real world" of…

  7. A Framing Primer for Community College Leaders

    Science.gov (United States)

    Nausieda, Ryan

    2014-01-01

    The purpose of this article is to be a tool for community college leaders, as well as campus members, to positively and effectively utilize framing on their campuses. The fictional case of Maggie Pascal at Midwestern Community College illustrates the process of framing the change of a new partnership with Wind Energy Corporation to internal…

  8. Framing Behaviours in Novice Interaction Designers

    Science.gov (United States)

    Lotz, Nicole; Sharp, Helen; Woodroffe, Mark; Blyth, Richard; Rajah, Dino; Ranganai, Turugare

    2015-01-01

    Framing design problems and solutions has been recognised in design studies as a central designerly activity. Some recent findings with expert designers relate framing practices to problem-solution co-evolution and analogy use, two further widely recognised design strategies. We wanted to understand if interaction design novices also use…

  9. Effect of framing on adolescents' decision making.

    Science.gov (United States)

    Chien, Y C; Lin, C; Worthley, J

    1996-12-01

    92 young adolescents were tested using Tversky and Kahneman's (1981) decision problems for framing effects. A notable number of young adolescents tested were not influenced by the context of the decision problems, thus they selected the same response option for positively and negatively framed problems. Parallel information was not available in Tversky and Kahneman's study for adults because they used a between-subjects design. However, for present adolescents who selected different response options for different framing problems, the response pattern exhibited by them resembled the general pattern exhibited by the adults tested in Tversky and Kahneman's study-negative frames led them to accept risk to avoid certain loss; positive frames prevented them from risking what they were certain to gain. Boys and girls were similar in their susceptibility to framing effects as were honors students in mathematics as compared to nonhonors students. Although the positive vs negative framing only influenced some of the young adolescents tested in this study, because the influence was consistent, researchers and educators interested in adolescents' decisions involving risky choices might use framing principles to design and assess cognitive interventions for high-risk behaviors among young adolescents.

  10. Sustainable food and agriculture: stakeholder's frames

    NARCIS (Netherlands)

    B. van Gorp; M.J. van der Goot

    2012-01-01

    Despite its importance, the notion of sustainability is open for discursive struggle. This article's primary objective is to acquire insight into the manner in which the principal stakeholders strategically use frames in their public communication about sustainable food and agriculture. A framing an

  11. News Framing in a Time of Terror

    DEFF Research Database (Denmark)

    Jørndrup, Hanne

    2016-01-01

    the attacks at the Charlie Hebdo office in Paris in January 2015. This article analyses how the Danish television channel DR1 framed the attacks in the newscast from the first shot at Krudttønden and for the following week. Furthermore, the analysis will discuss how the framing of the shooting as a “terror...

  12. Grasp Reading Skills to Improve Reading Ability?

    Institute of Scientific and Technical Information of China (English)

    CaoJingt

    2004-01-01

    Reading is a kind of communication, and for most people in most situations it is more important than speaking. For college students today, it is more important for them to obtain the newest information on their own fields through English,rather than showing their English certificates. However, the most common problem students have nowadays, including

  13. Integrating Reading and Writing through Extensive Reading

    Science.gov (United States)

    Park, Jeongyeon

    2016-01-01

    This study explores whether an extensive reading (ER) approach can enhance L2 learners' writing performance in an English for Academic Purposes context. Two classes were compared in terms of writing improvement after one semester: a 'traditional' writing class primarily focused on writing practice and grammar instruction, and an ER class in which…

  14. Science Fiction: Serious Reading, Critical Reading

    Science.gov (United States)

    Zigo, Diane; Moore, Michael T.

    2004-01-01

    Science fiction deserves a greater respect, serious and critical reading and a better place in high school literature classes. Some of the science fiction books by Isaac Asimov, Alfred Bester, Ray Bradbury and Octavia L. Butler and various activities for incorporating science fiction into the English language arts instruction classroom are…

  15. Simple Design Tool for Development of Well Insulated Window Frames and Optimization of the Frame Geometry

    DEFF Research Database (Denmark)

    Zajas, Jan Jakub; Heiselberg, Per

    2012-01-01

    This paper describes a design tool created with the purpose of designing highly insulated window frames. The design tool is based on a parametric model of the frame geometry, where various parameters describing the frame can be easily changed by the user. Based on this input, geometry of the fram...

  16. Approximately dual frames in Hilbert spaces and applications to Gabor frames

    DEFF Research Database (Denmark)

    Christensen, Ole; Laugesen, Richard S.

    2011-01-01

    Approximately dual frames are studied in the Hilbert space setting. Approximate duals are easier to construct than classical dual frames, and can be tailored to yield almost perfect reconstruction. Bounds on the deviation from perfect reconstruction are obtained for approximately dual frames cons...

  17. Numeracy and framing bias in epilepsy.

    Science.gov (United States)

    Choi, Hyunmi; Wong, John B; Mendiratta, Anil; Heiman, Gary A; Hamberger, Marla J

    2011-01-01

    Patients with epilepsy are frequently confronted with complex treatment decisions. Communicating treatment risks is often difficult because patients may have difficulty with basic statistical concepts (i.e., low numeracy) or might misconceive the statistical information based on the way information is presented, a phenomenon known as "framing bias." We assessed numeracy and framing bias in 95 adults with chronic epilepsy and explored cognitive correlates of framing bias. Compared with normal controls, patients with epilepsy had significantly poorer performance on the Numeracy scale (P=0.02), despite a higher level of education than normal controls (Pframing bias. Abstract problem solving performance correlated with the degree of framing bias (r=0.631, Pframing bias. Poor numeracy and susceptibility framing bias place patients with epilepsy at risk for uninformed decisions.

  18. Geometric Properties of Grassmannian Frames for and

    Directory of Open Access Journals (Sweden)

    Benedetto John J

    2006-01-01

    Full Text Available Grassmannian frames are frames satisfying a min-max correlation criterion. We translate a geometrically intuitive approach for two- and three-dimensional Euclidean space ( and into a new analytic method which is used to classify many Grassmannian frames in this setting. The method and associated algorithm decrease the maximum frame correlation, and hence give rise to the construction of specific examples of Grassmannian frames. Many of the results are known by other techniques, and even more generally, so that this paper can be viewed as tutorial. However, our analytic method is presented with the goal of developing it to address unresovled problems in -dimensional Hilbert spaces which serve as a setting for spherical codes, erasure channel modeling, and other aspects of communications theory.

  19. An introduction to frames and Riesz bases

    CERN Document Server

    Christensen, Ole

    2016-01-01

    This revised and expanded monograph presents the general theory for frames and Riesz bases in Hilbert spaces as well as its concrete realizations within Gabor analysis, wavelet analysis, and generalized shift-invariant systems.  Compared with the first edition, more emphasis is put on explicit constructions with attractive properties.  Based on the exiting development of frame theory over the last decade, this second edition now includes new sections on the rapidly growing fields of LCA groups, generalized shift-invariant systems, duality theory for as well Gabor frames as wavelet frames, and open problems in the field.   Key features include: *Elementary introduction to frame theory in finite-dimensional spaces * Basic results presented in an accessible way for both pure and applied mathematicians * Extensive exercises make the work suitable as a textbook for use in graduate courses * Full proofs includ ed in introductory chapters; only basic knowledge of functional analysis required * Explicit constructi...

  20. High frame rate CCD camera with fast optical shutter

    Energy Technology Data Exchange (ETDEWEB)

    Yates, G.J.; McDonald, T.E. Jr. [Los Alamos National Lab., NM (United States); Turko, B.T. [Lawrence Berkeley National Lab., CA (United States)

    1998-09-01

    A high frame rate CCD camera coupled with a fast optical shutter has been designed for high repetition rate imaging applications. The design uses state-of-the-art microchannel plate image intensifier (MCPII) technology fostered/developed by Los Alamos National Laboratory to support nuclear, military, and medical research requiring high-speed imagery. Key design features include asynchronous resetting of the camera to acquire random transient images, patented real-time analog signal processing with 10-bit digitization at 40--75 MHz pixel rates, synchronized shutter exposures as short as 200pS, sustained continuous readout of 512 x 512 pixels per frame at 1--5Hz rates via parallel multiport (16-port CCD) data transfer. Salient characterization/performance test data for the prototype camera are presented, temporally and spatially resolved images obtained from range-gated LADAR field testing are included, an alternative system configuration using several cameras sequenced to deliver discrete numbers of consecutive frames at effective burst rates up to 5GHz (accomplished by time-phasing of consecutive MCPII shutter gates without overlap) is discussed. Potential applications including dynamic radiography and optical correlation will be presented.

  1. Framework for risk analysis in Multimedia Environmental Systems (FRAMES)

    Energy Technology Data Exchange (ETDEWEB)

    Whelan, G.; Buck, J.W.; Castleton, K.J.; Hoopes, B.L.; Pelton, M.A.; McDonald, J.P.; Gelston, G.M.; Taira, R.Y. [Pacific Northwest National Lab., Richland, WA (United States)

    1998-05-01

    The objectives of this workshop are to (1) provide the NRC staff and the public with an overview of currently available Federally-Sponsored dose models appropriate for decommissioning assessments and (2) discuss NRC staff-developed questions related to model selection criteria with the final rule on ``Radiological Criteria for License Termination`` (62 FR 39058). For over 40 years, medium specific models have been and will continue to be developed in an effort to understand and predict environmental phenomena, including fluid-flow patterns, contaminant migration and fate, human or wildlife exposures, impacts from specific toxicants to specific species and their organs, cost-benefit analyses, impacts from remediation alternatives, etc. For nearly 40 years, medium-specific models have been combined for either sequential or concurrent assessments. The evolution of multiple-media assessment tools has followed a logic progression. To allow a suite of users the flexibility and versatility to construct, combine, and couple attributes that meet their specific needs without unnecessarily burdening the user with extraneous capabilities, the development of a computer-based methodology to implement a Risk Analysis in Multimedia Environmental Systems (FRAMES) was begun in 1994. FRAMES represents a platform which links elements together and yet does not represent the models that are linked to or within it; therefore, changes to elements that are linked to or within FRAMES do not change the framework.

  2. Frame-differencing methods for measuring bodily synchrony in conversation.

    Science.gov (United States)

    Paxton, Alexandra; Dale, Rick

    2013-06-01

    The study of interpersonal synchrony examines how interacting individuals grow to have similar behavior, cognition, and emotion in time. Many of the established methods of analyzing interpersonal synchrony are costly and time-consuming; the study of bodily synchrony has been especially laborious, traditionally requiring researchers to hand-code movement frame by frame. Because of this, researchers have been searching for more efficient alternatives for decades. Recently, some researchers (e.g., Nagaoka & Komori (IEICE Transactions on Information and Systems, 91(6), 1634-1640, 2008); Ramseyer & Tschacher, 2008) have applied computer science and computer vision techniques to create frame-differencing methods (FDMs) to simplify analyses. In this article, we provide a detailed presentation of one such FDM, created by modifying and adding to existing FDMs. The FDM that we present requires little programming experience or specialized equipment: Only a few lines of MATLAB code are required to execute an automated analysis of interpersonal synchrony. We provide sample code and demonstrate its use with an analysis of brief, friendly conversations; using linear mixed-effects models, the measure of interpersonal synchrony was found to be significantly predicted by time lag (p < .001) and by the interaction between time lag and measures of interpersonal liking (p < .001). This pattern of results fits with existing literature on synchrony. We discuss the current limitations and future directions for FDMs, including their use as part of a larger methodology for capturing and analyzing multimodal interaction.

  3. Parafoveal-on-Foveal Effects of Emotional Word Semantics in Reading Chinese Sentences: Evidence from Eye Movements

    Science.gov (United States)

    Yan, Ming; Sommer, Werner

    2015-01-01

    Despite the well-known influence of emotional meaning on cognition, relatively less is known about its effects on reading behavior. We investigated whether fixation behavior during the reading of Chinese sentences is influenced by emotional word meaning in the parafovea. Two-character target words embedded into the same sentence frames provided…

  4. Korean Learners' Metacognition in Reading Using Think-Aloud Procedures with a Focus on Regulation of Cognition

    Science.gov (United States)

    Kim, Hyang-Il; Cha, Kyung-Ae

    2015-01-01

    The primary goal of this study was to explore the changes that four Korean university students made in their regulation of cognition during reading processes. The students were trained using explicit reading strategy instruction based on the CALLA model. To this end, first, metacognition was framed and categorized by the definition from Baker and…

  5. Role of Reading Engagement in Mediating Effects of Reading Comprehension Instruction on Reading Outcomes

    Science.gov (United States)

    Wigfield, Allan; Guthrie, John T.; Perencevich, Kathleen C.; Taboada, Ana; Klauda, Susan Lutz; McRae, Angela; Barbosa, Pedro

    2008-01-01

    The engagement model of reading development suggests that instruction improves students' reading comprehension to the extent that it increases students' engagement processes in reading. We compared how Concept-Oriented Reading Instruction (CORI) (support for cognitive and motivational processes in reading), strategy instruction (support for…

  6. Forecasting Reading Anxiety for Promoting English-Language Reading Performance Based on Reading Annotation Behavior

    Science.gov (United States)

    Chen, Chih-Ming; Wang, Jung-Ying; Chen, Yong-Ting; Wu, Jhih-Hao

    2016-01-01

    To reduce effectively the reading anxiety of learners while reading English articles, a C4.5 decision tree, a widely used data mining technique, was used to develop a personalized reading anxiety prediction model (PRAPM) based on individual learners' reading annotation behavior in a collaborative digital reading annotation system (CDRAS). In…

  7. The Relationships between Korean University Students' Reading Attitude, Reading Strategy Use, and Reading Proficiency

    Science.gov (United States)

    Kim, Hyangil

    2016-01-01

    This present study investigated the relationships among L2 readers' reading attitude, reading strategy use, and reading proficiency in order to identify patterns caused by individuals' differences. For this study, 153 Korean university students replied to a reading attitude and reading strategy questionnaire. An ANOVA and frequency analysis were…

  8. Informative-frame filtering in endoscopy videos

    Science.gov (United States)

    An, Yong Hwan; Hwang, Sae; Oh, JungHwan; Lee, JeongKyu; Tavanapong, Wallapak; de Groen, Piet C.; Wong, Johnny

    2005-04-01

    Advances in video technology are being incorporated into today"s healthcare practice. For example, colonoscopy is an important screening tool for colorectal cancer. Colonoscopy allows for the inspection of the entire colon and provides the ability to perform a number of therapeutic operations during a single procedure. During a colonoscopic procedure, a tiny video camera at the tip of the endoscope generates a video signal of the internal mucosa of the colon. The video data are displayed on a monitor for real-time analysis by the endoscopist. Other endoscopic procedures include upper gastrointestinal endoscopy, enteroscopy, bronchoscopy, cystoscopy, and laparoscopy. However, a significant number of out-of-focus frames are included in this type of videos since current endoscopes are equipped with a single, wide-angle lens that cannot be focused. The out-of-focus frames do not hold any useful information. To reduce the burdens of the further processes such as computer-aided image processing or human expert"s examinations, these frames need to be removed. We call an out-of-focus frame as non-informative frame and an in-focus frame as informative frame. We propose a new technique to classify the video frames into two classes, informative and non-informative frames using a combination of Discrete Fourier Transform (DFT), Texture Analysis, and K-Means Clustering. The proposed technique can evaluate the frames without any reference image, and does not need any predefined threshold value. Our experimental studies indicate that it achieves over 96% of four different performance metrics (i.e. precision, sensitivity, specificity, and accuracy).

  9. Student-Centered Reading Activities.

    Science.gov (United States)

    Moffett, James; Wagner, Betty Jane

    1991-01-01

    Offers student-centered reading activities designed to bring students to reading maturity and involvement in literature. Discusses partner reading, dramatizing and performing texts, transforming texts, journal writing, discussion, and writing. (PRA)

  10. Discourse Awareness and Teaching Reading

    Institute of Scientific and Technical Information of China (English)

    FEI Jun

    2014-01-01

    Reading is an interactive process and discourse knowldege facilitates interpretation of the text. This paper discusses the thoery of reading from a discouse apporach, explores the interactive approach of teaching reading and discusses pedagogical impi-cations with contexts concerned.

  11. High frame rate measurements of semiconductor pixel detector readout IC

    Science.gov (United States)

    Szczygiel, R.; Grybos, P.; Maj, P.

    2012-07-01

    We report on high count rate and high frame rate measurements of a prototype IC named FPDR90, designed for readouts of hybrid pixel semiconductor detectors used for X-ray imaging applications. The FPDR90 is constructed in 90 nm CMOS technology and has dimensions of 4 mm×4 mm. Its main part is a matrix of 40×32 pixels with 100 μm×100 μm pixel size. The chip works in the single photon counting mode with two discriminators and two 16-bit ripple counters per pixel. The count rate per pixel depends on the effective CSA feedback resistance and can be set up to 6 Mcps. The FPDR90 can operate in the continuous readout mode, with zero dead time. Due to the architecture of digital blocks in pixel, one can select the number of bits read out from each counter from 1 to 16. Because in the FPDR90 prototype only one data output is available, the frame rate is 9 kfps and 72 kfps for 16 bits and 1 bit readout, respectively (with nominal clock frequency of 200 MHz).

  12. High frame rate measurements of semiconductor pixel detector readout IC

    Energy Technology Data Exchange (ETDEWEB)

    Szczygiel, R., E-mail: robert.szczygiel@agh.edu.pl [AGH University of Science and Technology, Department of Measurement and Instrumentation, Al. Mickiewicza 30, 30-059 Cracow (Poland); Grybos, P.; Maj, P. [AGH University of Science and Technology, Department of Measurement and Instrumentation, Al. Mickiewicza 30, 30-059 Cracow (Poland)

    2012-07-11

    We report on high count rate and high frame rate measurements of a prototype IC named FPDR90, designed for readouts of hybrid pixel semiconductor detectors used for X-ray imaging applications. The FPDR90 is constructed in 90 nm CMOS technology and has dimensions of 4 mm Multiplication-Sign 4 mm. Its main part is a matrix of 40 Multiplication-Sign 32 pixels with 100 {mu}m Multiplication-Sign 100 {mu}m pixel size. The chip works in the single photon counting mode with two discriminators and two 16-bit ripple counters per pixel. The count rate per pixel depends on the effective CSA feedback resistance and can be set up to 6 Mcps. The FPDR90 can operate in the continuous readout mode, with zero dead time. Due to the architecture of digital blocks in pixel, one can select the number of bits read out from each counter from 1 to 16. Because in the FPDR90 prototype only one data output is available, the frame rate is 9 kfps and 72 kfps for 16 bits and 1 bit readout, respectively (with nominal clock frequency of 200 MHz).

  13. DNA, dichotomic classes and frame synchronization: a quasi-crystal framework.

    Science.gov (United States)

    Giannerini, Simone; Gonzalez, Diego Luis; Rosa, Rodolfo

    2012-06-28

    In this article, we show how a new mathematical model of the genetic code can be exploited for investigating the almost periodic properties of DNA and mRNA protein-coding sequences. We present the main mathematical features of the model and highlight its connections with both number theory and group theory. The group theoretic framework presents interesting analogies with the theory of crystals. Moreover, we exploit the information provided by dichotomic classes, binary variables naturally derived from the mathematical model, in order to build statistical classifiers for retrieving and predicting the normal reading frame used by the ribosome in protein synthesis. The results show that coding sequences possess a local informational structure that can be related to frame synchronization processes. The information for retrieving the normal reading frame, which implies the existence of short-range correlations and almost periodic structures related to the organization of codons, offers an interesting analogy with the properties of quasi-crystals. From a theoretical point of view, our results might contribute to clarifying the relation between biological information and shape in nucleic acids and proteins. Also, from the point of view of applications, we present new promising tools for designing efficient algorithms for frame synchronization, which plays a crucial role in faithful synthesis of proteins.

  14. New oxazilines with sulfolane frame

    Directory of Open Access Journals (Sweden)

    Iryna S. Zarovna

    2014-03-01

    Full Text Available Oxazolines are widely used as synthons for medicines and their production, as protection for structural fragments of reaction centers, as well as ligands. We for the first time examined the ability of cis- and trans-3-hydroxy-4-aminosulfolanov to form oxazolines. Oxazolines are formed by cyclization of the corresponding N-acyl derivatives of trans-3-hydroxy-4-aminosulfolan under reflux in thionyl chloride; amides of cis-isomer aminoalcohol under the same conditions of the reaction do not formed identically product. These results confirm that the oxazoline cycle formed by realization of intramolecular SN2- mechanism using thionyl chloride as dehydrating agent; alternative mechanism (the attack of the hydroxyl group on carbon of amide group can not be realized using the microwave radiation reaction mass.

  15. Lenses on Reading An Introduction to Theories and Models

    CERN Document Server

    Tracey, Diane H

    2012-01-01

    This widely adopted text explores key theories and models that frame reading instruction and research. Readers learn why theory matters in designing and implementing high-quality instruction and research; how to critically evaluate the assumptions and beliefs that guide their own work; and what can be gained by looking at reading through multiple theoretical lenses. For each theoretical model, classroom applications are brought to life with engaging vignettes and teacher reflections. Research applications are discussed and illustrated with descriptions of exemplary studies. New to This Edition

  16. Benefit-risk Evaluation for Diagnostics: A Framework (BED-FRAME).

    Science.gov (United States)

    Evans, Scott R; Pennello, Gene; Pantoja-Galicia, Norberto; Jiang, Hongyu; Hujer, Andrea M; Hujer, Kristine M; Manca, Claudia; Hill, Carol; Jacobs, Michael R; Chen, Liang; Patel, Robin; Kreiswirth, Barry N; Bonomo, Robert A

    2016-09-15

    The medical community needs systematic and pragmatic approaches for evaluating the benefit-risk trade-offs of diagnostics that assist in medical decision making. Benefit-Risk Evaluation of Diagnostics: A Framework (BED-FRAME) is a strategy for pragmatic evaluation of diagnostics designed to supplement traditional approaches. BED-FRAME evaluates diagnostic yield and addresses 2 key issues: (1) that diagnostic yield depends on prevalence, and (2) that different diagnostic errors carry different clinical consequences. As such, evaluating and comparing diagnostics depends on prevalence and the relative importance of potential errors. BED-FRAME provides a tool for communicating the expected clinical impact of diagnostic application and the expected trade-offs of diagnostic alternatives. BED-FRAME is a useful fundamental supplement to the standard analysis of diagnostic studies that will aid in clinical decision making.

  17. Framing of Issues Across Actors: Exploring Competing Discourses in Digital Arenas

    DEFF Research Database (Denmark)

    Ravazzani, Silvia; Maier, Carmen Daniela

    2017-01-01

    . It also responds to “the need for critical awareness of discourse in contemporary society” (Fairclough, 2010, p. 554) by revealing how the power positions of “challengers and powerholders” (Steinberg, 1998, p. 846) are discursively reproduced and reinforced through distinctive discursive and interactive......Purpose To show how the strategic selection of discursive and interactive strategies generates specific framings of an issue to advocate opposite positions, embodying a struggle of power between parties with their own agendas. Design/methodology/approach Drawing on literature within framing...... on Mauna Kea Mountain in Hawaii. Findings On each website, frame articulation attempts to connect the event to specific concerns, values and beliefs in order to construct alternative versions of reality which can possibly fit with those of supporters. Simultaneously, this is reinforced by frame...

  18. Metacognition and Reading Comprehension

    Institute of Scientific and Technical Information of China (English)

    Cecilia Chang

    2000-01-01

    @@ Introduction Reading is a mysterious process that has attracted attention from psychologists, reading researchers and educators alike for decades. Currently, reading is viewed as a meaningconstructing process where the reader interacts with the text by simultaneously using information from a variety of sources ranging from one's background knowledge of the content and about the world to the knowledge about the language in which the text is written (Mulling, 1994). Moreover, reading comprehension is achieved only after skillful orchestration of all the resources the reader possess when engaged in the act of reading. Among the various kinds of strategies a reader needs during the reading process are the monitoring strategies. In order to fully utilize the strategies, the reader needs to possess good metacognition. Metacognition refers to one's deliberate conscious control of one's cognition actions (Brown, 1980). Since it is crucial for success that we know what we know and what we don't know and, consequently what to do about what we know and don't know, it is not surprising to find the notion of metacognition being stressed in almost every situation of learning. Given the importance of metacognition in successful learning, the purpose of this paper is to explore the role metacognition plays in reading comprehension, and consequently, identify future research directions.

  19. Reading between eye saccades.

    Directory of Open Access Journals (Sweden)

    Caroline Blais

    Full Text Available BACKGROUND: Skilled adult readers, in contrast to beginners, show no or little increase in reading latencies as a function of the number of letters in words up to seven letters. The information extraction strategy underlying such efficiency in word identification is still largely unknown, and methods that allow tracking of the letter information extraction through time between eye saccades are needed to fully address this question. METHODOLOGY/PRINCIPAL FINDINGS: The present study examined the use of letter information during reading, by means of the Bubbles technique. Ten participants each read 5,000 five-letter French words sampled in space-time within a 200 ms window. On the temporal dimension, our results show that two moments are especially important during the information extraction process. On the spatial dimension, we found a bias for the upper half of words. We also show for the first time that letter positions four, one, and three are particularly important for the identification of five-letter words. CONCLUSIONS/SIGNIFICANCE: Our findings are consistent with either a partially parallel reading strategy or an optimal serial reading strategy. We show using computer simulations that this serial reading strategy predicts an absence of a word-length effect for words from four- to seven letters in length. We believe that the Bubbles technique will play an important role in further examining the nature of reading between eye saccades.

  20. The constructionist approach to framing: Bringing culture back in

    OpenAIRE

    Van Gorp, Baldwin

    2007-01-01

    This article aims, within the constructionist paradigm, at integrating culture into the framing process. Four characteristics are important for this approach: the distinction between the event, the media content, and the frame; the explicit attention to the reconstruction of frame packages; the relationship between frame packages and cultural phenomena; and the interaction between frame sponsors, key events, media content, schemata, and the stock of frames. An elaborated framing model is pres...

  1. Newspaper Reading and English Teaching

    Institute of Scientific and Technical Information of China (English)

    霍丽蓉

    2014-01-01

    Reading is not only the most effective way to get the knowledge of the language but also the only way to improve the language skills of English learners. However, the vast majority of students' reading ability are far apart from the curriculum stan⁃dards in reality. Reading English newspaper helps to mobilize the enthusiasm of students' reading, and improve their reading abili⁃ty. It forms a bridge between learning and real life. It extends the English reading from the teaching materials and guides students to a lot of extracurricular reading, creating a new reading space for students.

  2. Interruptions disrupt reading comprehension.

    Science.gov (United States)

    Foroughi, Cyrus K; Werner, Nicole E; Barragán, Daniela; Boehm-Davis, Deborah A

    2015-06-01

    Previous research suggests that being interrupted while reading a text does not disrupt the later recognition or recall of information from that text. This research is used as support for Ericsson and Kintsch's (1995) long-term working memory (LT-WM) theory, which posits that disruptions while reading (e.g., interruptions) do not impair subsequent text comprehension. However, to fully comprehend a text, individuals may need to do more than recognize or recall information that has been presented in the text at a later time. Reading comprehension often requires individuals to connect and synthesize information across a text (e.g., successfully identifying complex topics such as themes and tones) and not just make a familiarity-based decision (i.e., recognition). The goal for this study was to determine whether interruptions while reading disrupt reading comprehension when the questions assessing comprehension require participants to connect and synthesize information across the passage. In Experiment 1, interruptions disrupted reading comprehension. In Experiment 2, interruptions disrupted reading comprehension but not recognition of information from the text. In Experiment 3, the addition of a 15-s time-out prior to the interruption successfully removed these negative effects. These data suggest that the time it takes to process the information needed to successfully comprehend text when reading is greater than that required for recognition. Any interference (e.g., an interruption) that occurs during the comprehension process may disrupt reading comprehension. This evidence supports the need for transient activation of information in working memory for successful text comprehension and does not support LT-WM theory.

  3. I read, you read, we read: the history of reading in Slovenia

    Directory of Open Access Journals (Sweden)

    Anja Dular

    2013-03-01

    Full Text Available ABSTRACTPurpose: The aim of the article is to research reading habits in Slovenia in the period between 16th and 19th century and to find similarities with Austria and other European countries of that time.Methodology/approach: For the purpose of the analysis different resources were used – study books, catechisms, prayer books and manuals. We were focused on introductions in which readers are advised how to read, explaining to whom the work is intended and emphasizing the importance of meditation on the texts.Results: Historically the laud reading was prefered, as to continue the folk tradition. However, the 16th century texts were transmitted by women while the folk tradition was narrated by males. In the 18th century the higher level of literacy and greater book production and availability caused that the books were not a privilege of a few. At that time more texts were intended for silent, individual reading. Interestingly, the authors emphasized the importance of meditation on the texts, too. It was also advised when to read – it wasrecommedend to read in leisure time on Sundays, and on holidays. The role of books was also to breakaway with the reality and to forget everyday problems. Due to the overproduction of books in the 17th centrury it was concerned that books are misleading the crowds. The church considered the reading of books as inappropriate, and criticized fiction, novels and adventure stories mostly read by women.Research limitation: The study is based on Slovenian texts only, although the foreign literature, especially in German, was generally available, too.Originality/practical implications: The study is fullfiling the gap in the history of reading in Slovenia.

  4. Recursive Frame Analysis: Reflections on the Development of a Qualitative Research Method

    Science.gov (United States)

    Keeney, Hillary; Keeney, Bradford

    2012-01-01

    The origin of recursive frame analysis (RFA) is revisited and discussed as a postmodern alternative to modernist therapeutic models and research methods that foster hegemony of a preferred therapeutic metaphor, narrative, or strategy. It encourages improvisational performance while enabling a means of scoring the change and movement of the…

  5. Myths, Misconceptions, and Misunderstandings: A Different Spin on Coriolis--Applying Frame of Reference

    Science.gov (United States)

    DiSpezio, Michael A.

    2011-01-01

    This article addresses misconceptions surrounding the Coriolis force and describes how it should be presented as a function within inertial and noninertial frames of reference. Not only does this demonstrate the nature of science as it strives to best interpret the natural world (and presents alternative explanations), but it offers a rich…

  6. q-Besselian Frames in Banach Spaces

    Institute of Scientific and Technical Information of China (English)

    Yu Can ZHU

    2007-01-01

    In this paper, we introduce the concepts of q-Besselian frame and (p, σ)-near Riesz basis in a Banach space, where σ is a finite subset of positive integers and 1/p + 1/q=1 with p 1, q 1, and determine the relations among q-frame, p-Riesz basis, q-Besselian frame and (p, σ)-near Riesz basis in a Banach space. We also give some sufficient and necessary conditions on a q-Besselian frame for a Banach space. In particular, we prove reconstruction formulas for Banach1 spaces X and X* that if {Xn}∞n=1(∪)X is a q-Besselian frame for X, then there exists a p-Besselian frame {y*n}∞n=1(∪) X* for X* such that x = ∑∞n-1 y*n(x)xn for all x ∈ X, and x = ∑∞n-1 x*xny*n, for all x* ∈ X*. Lastly, we consider the stability of a q-Besselian frame for the Banach space X under perturbation. Some results of J. R. Holub, P. G. Casazza, O. Christensen and others in Hilbert spaces are extended to Banach spaces.

  7. Bound and Radiation Fields in Rindler Frame

    CERN Document Server

    Hirayama, T

    2001-01-01

    Energy-momentum tensor of Li\\'enard-Wiechert field is split into bound and emitted parts in Rindler frame, by generalizing the reasoning of Teitelboim used in inertial frame. Our discussion proceeds with the the notion of ``energy'' defined with respect to the Killing vector field attached to the frame. We obtain the ``radiation formula in Rindler frame''(Rindler version of Larmor formula) and it is found that the radiation power is proportional to the square of ``acceleration $\\alpha^\\mu$ of the charge relative to the Rindler frame''. This result makes us split the Li\\'enard-Wiechert field into the part $\\tII$ which is linear in $\\alpha^\\mu$ and the part $\\tI$ which is independent of $\\alpha^\\mu$, and by using them, we split the energy-momentum tensor into two parts. We find that these are properly interpreted as the emitted and bound parts of the tensor in Rindler frame. In our identification of radiation, a charge does not radiate not only in the case where the charge is fixed to the Rindler frame, but als...

  8. Quantum Stackelberg Duopoly in a Noninertial Frame

    Institute of Scientific and Technical Information of China (English)

    Salman Khan; M. Khalid Khan

    2011-01-01

    We study the influence of the Unruh effect on quantum Stackelberg duopoly.It is shown that the acceleration of a noninertial frame strongly affects the payoffs of the firms. The validation of the subgame perfect Nash equilibrium is limited to a particular range of acceleration of the noninertial frame.The benefit of the initial state entanglement in the quantum form of the duopoly in the inertial frame is adversely affected by the acceleration.The duopoly can become a follower advantage only in a small region of the acceleration.

  9. Groups, cacti and framed little discs

    CERN Document Server

    Hepworth, Richard

    2010-01-01

    Let G be a topological group. Then the based loopspace of G is an algebra over the cacti operad, while the double loopspace of the classifying space of G is an algebra over the framed little discs operad. This paper shows that these two algebras are equivalent, in the sense that they are weakly equivalent E-algebras, where E is an operad weakly equivalent to both framed little discs and cacti. We recover the equivalence between cacti and framed little discs, and Menichi's isomorphism between the BV-algebras obtained by taking the homology of the loopspace of G and of the double loopspace of BG.

  10. Framing of regimes and transition strategies

    DEFF Research Database (Denmark)

    Jensen, Jens Stissing

    2012-01-01

    This article suggests that transition strategies are always formulated in the context of specific representations of the regime and the challenges it faces. It is argued that the framing of a regime affects the envisioning of transition strategies. An analysis of the current development agenda...... for the housing construction sector in Denmark reveals the relevance and impacts of different regime framings. It is proposed that the ability to cope with framing issues as situated and political processes is at the core of the governance of transitions....

  11. Elastoplastic analysis of knee bracing frame

    Institute of Scientific and Technical Information of China (English)

    HUANG Zhen; LI Qing-song; CHEN Long-zhu

    2005-01-01

    The knee bracing steel frame (KBF) is a new kind of energy dissipating frame, which combines excellent ductility and lateral stiffness. As the structural fuse of the frame, the knee element will yield first during a severe earthquake so that no damage occurs to the major structural members and the rehabilitation is easy and economical. To help fully understand the relations between its seismic performance and the structural parameters, systematic elastoplastic analysis of the KBF structure with finite element method was conducted in this work. Finally, general design recommendations were made according to the results of the analysis.

  12. Parametric Study Of Window Frame Geometry

    DEFF Research Database (Denmark)

    Zajas, Jan Jakub; Heiselberg, Per

    2013-01-01

    This paper describes a parametric study on window frame geometry with the goal of designing frames with very good thermal properties. Three different parametric frame models are introduced, deseribed by a number of variables. In the first part of the study, a process of sensitivity analysis is co...... energy gain factor and minimizing the material use. Since the objectives contradiet each other, it was found that it is not possible to identifY a single solution that satisfies all these goals. lnstead, a compromise between the objectives has to be found....

  13. Reading Acts: An Inquiry into Reading and Teaching

    Science.gov (United States)

    Sams, Brandon L.

    2012-01-01

    This text performs reading for teaching in an audit culture. Two teachers, myself and Steven, read the memoir "Hole in My Life" by Jack Gantos and, while reading, recorded our experiences as readers and planned to teach the book to Steven's English class. This study is an inquiry into the phenomenon of "reading to teach,"…

  14. Reading Motivation and Reading Engagement: Clarifying Commingled Conceptions

    Science.gov (United States)

    Unrau, Norman J.; Quirk, Matthew

    2014-01-01

    The constructs of motivation for reading and reading engagement have frequently become blurred and ambiguous in both research and discussions of practice. To address this commingling of constructs, the authors provide a concise review of the literature on motivation for reading and reading engagement and illustrate the blurring of those concepts…

  15. Child-centered reading intervention: See, talk, dictate, read, write!

    Directory of Open Access Journals (Sweden)

    Muhammet BAŞTUĞ

    2016-06-01

    Full Text Available Poor reading achievement of children in elementary schools has been one of the major concerns in education. The aim of this study is to examine the effectiveness of a child-centered reading intervention in eliminating the reading problems of a student with poor reading achievement. The research was conducted with a student having difficulty in reading. A reading intervention was designed that targeted multiple areas of reading and aimed to improve reading skills through the use of multiple strategies. This intervention is child-centered and includes visual aids, talking, dictating, reading and writing stages. The study was performed in 35 sessions consisting of stages of a single sentence (5 sessions, two sentences (5 sessions, three sentences (20 sessions and the text stage (5 sessions. The intervention sessions were audio-taped. These recordings and the written responses to the reading comprehension questions provided the data for analysis. The findings on the reading intervention revealed positive outcomes. The student exhibited certain improvements at the levels of reading, reading rate and reading comprehension. These results were discussed in the literature and the findings suggest that child-centered reading strategies such as talking, dictating and writing should be the main focus of instruction for students with low reading literacy achievement to enable these students to meet the demands of the curriculum.

  16. Developing New Reading Assessments to Promote Beginning Reading in Singapore

    Science.gov (United States)

    Koh, Kim H.; Paris, Scott G.

    2011-01-01

    Effective reading instruction and intervention are rooted in effective assessments of children's developing skills in reading. The article aims to describe the development of new reading assessments to help promote beginning reading in Singapore primary schools. We begin with an introduction to the educational landscape and policies before…

  17. Teen Summer Reading Program, 1999. Read around the World.

    Science.gov (United States)

    Virginia State Library, Richmond.

    This guide for the 1999 Virginia teen summer reading program for public libraries, "Read around the World," includes the following chapters: (1) "Reading and Teens," including serving the underserved, tips for teens, and a recipe for choosing a book to read for fun; (2) "Programming and Teens," including "The Why…

  18. How to read efficiently%How to read Efficiently

    Institute of Scientific and Technical Information of China (English)

    肖平

    2007-01-01

    At present, the university graduates have mountains of books to read. For this phenomenon, this paper first indicates why the students must read extensively and then tentatively suggests three measures establish new concept in reading, practise reading pragmatically, review and exchange.

  19. The cold reading technique.

    Science.gov (United States)

    Dutton, D L

    1988-04-15

    For many people, belief in the paranormal derives from personal experience of face-to-face interviews with astrologers, palm readers, aura and Tarot readers, and spirit mediums. These encounters typically involve cold reading, a process in which a reader makes calculated guesses about a client's background and problems and, depending on the reaction, elaborates a reading which seems to the client so uniquely appropriate that it carries with it the illusion of having been produced by paranormal means. The cold reading process is shown to depend initially on the Barnum effect, the tendency for people to embrace generalized personality descriptions as idiosyncratically their own. Psychological research into the Barnum effect is critically reviewed, and uses of the effect by a professional magician are described. This is followed by detailed analysis of the cold reading performances of a spirit medium. Future research should investigate the degree to which cold readers may have convinced themselves that they actually possess psychic or paranormal abilities.

  20. Sequence Read Archive (SRA)

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Sequence Read Archive (SRA) stores raw sequencing data from the next generation of sequencing platforms including Roche 454 GS System®, Illumina Genome...

  1. Art Criticism and Reading.

    Science.gov (United States)

    Feldman, Edmund Burke; Woods, Don

    1981-01-01

    The authors review a body of theory and accumulating evidence which suggests that critical study of the arts facilitates the development of cognitive skills, including those essential to reading. (Author/SJL)

  2. Reading the Tourist Guidebook

    DEFF Research Database (Denmark)

    Therkelsen, Anette; Sørensen, Anders

    2005-01-01

    This article investigates tourists’ ways of reading their guidebooks on the basis of qualitative interviews with tourists visiting Copenhagen, Denmark. Tourist guidebooks have only been dealt with sporadically by tourism scholars. The relatively few studies that focus on guidebooks either present...... a historical perspective on the guidebook or centre on content analyses of place representation, whereas virtually no research exists on the way in which tourists read and use their guidebooks. This study reveals that tourists read the same guidebooks in a number of different ways regarding types...... of information sought, amount of information read and level of involvement displayed, indicating a three-pronged typology of guidebook readers. The guidebook reader typology thus constructed may be regarded as a first step in understanding the effect of guidebooks on tourists’ behaviour and their experience...

  3. Assessment of Reading Comprehension

    Directory of Open Access Journals (Sweden)

    Madani HABIB

    2016-06-01

    Full Text Available This study attempts to shed light on the concept of assessment as an essential pedagogical practice for the improvement of the teaching-learning process. Particularly, it stresses the strategies and the techniques that should be used in assessing reading comprehension with reference to EFL classrooms. It describes the kinds of tasks that actually reveal students’ reading comprehension abilities and needs. Moreover, this paper aims to illustrate the types and the advantages of assessment for both teachers and learners. More importantly, this study tries to bring equitable evidence of how reading comprehension can be adequately assessed. The findings showed that assessment of reading comprehension is central to English language teaching as it provides teachers with essential information about students’ weaknesses, needs, obstacles, and deficits. Thus, teachers can implement the appropriate techniques and use the assessment results to amend their classroom instruction and enhance the learning abilities.

  4. Reading-Boxing Class

    Science.gov (United States)

    Kravitz, Richard; Shapiro, Marvin

    1969-01-01

    The physical education department of the Pennsylvania Advancement School of Philadelphia has established a reading and communication skill project that uses the appeal of sports to help students improve their basic skills. (Author)

  5. Metacognition in Reading Comprehension

    OpenAIRE

    Ceylan, Eda; Harputlu, Leyla

    2015-01-01

    Metacognition is defined basically as thinking about thinking. It is a significant factor that affects many activities related to language use. Reading comprehension, which is an indispensable part of daily life and language classrooms, is affected by metacognition, too. Hence, this paper aims to present an overview of the recent theoretical and empirical studies about metacognition and reading comprehension. Firstly, it provides the definitions and the importance of metacognition. Secondly, ...

  6. Looking for an Alternative.

    Science.gov (United States)

    Kennedy, Jack

    1999-01-01

    Argues that high school newspapers might do well to create stronger ties with alternative weeklies. Discusses issues of niche marketing, alternative content, and alternative presentation. Notes that high school papers could learn a lot from alternative newspapers. (SR)

  7. extendFromReads

    Energy Technology Data Exchange (ETDEWEB)

    2013-10-03

    This package assists in genome assembly. extendFromReads takes as input a set of Illumina (eg, MiSeq) DNA sequencing reads, a query seed sequence and a direction to extend the seed. The algorithm collects all seed--]matching reads (flipping reverse--]orientation hits), trims off the seed and additional sequence in the other direction, sorts the remaining sequences alphabetically, and prints them aligned without gaps from the point of seed trimming. This produces a visual display distinguishing the flanks of multi-]copy seeds. A companion script hitMates.pl collects the mates of seed--]hi]ng reads, whose alignment reveals longer extensions from the seed. The collect/trim/sort strategy was made iterative and scaled up in the script denovo.pl, for de novo contig assembly. An index is pre--]built using indexReads.pl that for each unique 21--]mer found in all the reads, records its gfateh of extension (whether extendable, blocked by low coverage, or blocked by branching after a duplicated sequence) and other characteristics. Importantly, denovo.pl records all branchings that follow a branching contig endpoint, providing contig-]extension information

  8. Mappability and Read Length

    Directory of Open Access Journals (Sweden)

    Wentian eLi

    2014-11-01

    Full Text Available Power-law distributions are the main functional form forthe distribution of repeat size and repeat copy number in the human genome. When the genome is broken into fragments for sequencing, the limited size offragments and reads may prevent an unique alignment of repeatsequences to the reference sequence. Repeats in the human genome canbe as long as $10^4$ bases, or $10^5-10^6$ bases when allowing for mismatches between repeat units. Sequence reads from these regions are therefore unmappable when the read length is in the range of $10^3$ bases.With the read length of exactly 1000 bases, slightly more than 1% of theassembled genome, and slightly less than 1% of the 1kbreads, are unmappable, excluding the unassembled portion of the humangenome (8% in GRCh37. The slow decay (long tail ofthe power-law function implies a diminishing return in convertingunmappable regions/reads to become mappable with the increase of theread length, with the understanding that increasing read length willalways move towards the direction of 100% mappability.

  9. News Media Framing of Negative Campaigning

    DEFF Research Database (Denmark)

    Pedersen, Rasmus Tue

    2014-01-01

    that news coverage of negative campaigning does apply the strategic game frame to a significantly larger degree than articles covering positive campaigning. This finding has significant implications for campaigning politicians and for scholars studying campaign and media effects.......News media coverage of election campaigns is often characterized by use of the strategic game frame and a focus on politicians’ use of negative campaigning. However, the exact relationship between these two characteristics of news coverage is largely unexplored. This article theorizes that consumer...... demand and norms of journalistic independence might induce the news media outlets to cover negative campaigning with a strategic game frame. A comprehensive content analysis based on several newspaper types, several election campaigns, and several different measurements of media framing confirms...

  10. Superluminal Phenomena and the Quantum Preferred Frame

    CERN Document Server

    Rembielinski, J

    2000-01-01

    Motivated by a number of recent experiments, we discuss in this paper a speculative but physically admissible form and solutions of effective Maxwell-like equations describing propagation of electromagnetic field in a medium which ``feels'' a quantum preferred frame.

  11. Framing effect following bilateral amygdala lesion.

    Science.gov (United States)

    Talmi, Deborah; Hurlemann, René; Patin, Alexandra; Dolan, Raymond J

    2010-05-01

    A paradigmatic example of an emotional bias in decision making is the framing effect, where the manner in which a choice is posed--as a potential loss or a potential gain--systematically biases an ensuing decision. Two fMRI studies have shown that the activation in the amygdala is modulated by the framing effect. Here, contrary to an expectation based on these studies, we show that two patients with Urbach-Wiethe (UW) disease, a rare condition associated with congenital, complete bilateral amygdala degeneration, exhibit an intact framing effect. However, choice preference in these patients did show a qualitatively distinct pattern compared to controls evident in an increased propensity to gamble, indicating that loss of amygdala function does exert an overall influence on risk-taking. These findings suggest either that amygdala does contribute to decision making but does not play a causal role in framing, or that UW is not a pure lesion model of amygdala function.

  12. Gabor frames by sampling and periodization

    DEFF Research Database (Denmark)

    Søndergaard, Peter Lempel

    2007-01-01

    By sampling the window of Gabor frame for L-2 (R) belonging to Feichtingers algebra S-0 (R), one obtains a Gabor frame for l(2) (Z). In this article we present a survey of results by R. Orr and A.J.E.M. Janssen and extend their ideas to cover interrelations among Gabor frames for the four spaces L......-2 (R), l(2) (Z), L-2 ([O,L]) and C-L. Some new results about the general dual windows with respect to sampling and periodization are presented as well. This theory is used to show a new result of the Kaiblinger type to construct an approximation to the canonical dual window of a Gabor frame for L-2...

  13. Lifting scheme of symmetric tight wavelets frames

    Institute of Scientific and Technical Information of China (English)

    ZHUANG BoJin; YUAN WeiTao; PENG LiZhong

    2008-01-01

    This paper proposes a method to realize the lifting scheme of tight frame wavelet filters. As for 4-channel tight frame wavelet filter, the tight frame transforms' ma-trix is 2×4, but the lifting scheme transforms' matrix must be 4×4. And in the case of 3-channel tight frame wavelet filter, the transforms' matrix is 2×3, but the lifting scheme transforms' matrix must be 3×3. In order to solve this problem, we intro-duce two concepts: transferred polyphase matrix for 4-channel filters and trans-ferred unitary matrix for 3-channel filters. The transferred polyphase matrix is sym-metric/antisymmetric. Thus, we use this advantage to realize the lifting scheme.

  14. Quantization Noise Shaping on Arbitrary Frame Expansions

    Directory of Open Access Journals (Sweden)

    Boufounos Petros T

    2006-01-01

    Full Text Available Quantization noise shaping is commonly used in oversampled A/D and D/A converters with uniform sampling. This paper considers quantization noise shaping for arbitrary finite frame expansions based on generalizing the view of first-order classical oversampled noise shaping as a compensation of the quantization error through projections. Two levels of generalization are developed, one a special case of the other, and two different cost models are proposed to evaluate the quantizer structures. Within our framework, the synthesis frame vectors are assumed given, and the computational complexity is in the initial determination of frame vector ordering, carried out off-line as part of the quantizer design. We consider the extension of the results to infinite shift-invariant frames and consider in particular filtering and oversampled filter banks.

  15. Compactly supported frames for decomposition spaces

    DEFF Research Database (Denmark)

    Nielsen, Morten; Rasmussen, Kenneth Niemann

    2012-01-01

    In this article we study a construction of compactly supported frame expansions for decomposition spaces of Triebel-Lizorkin type and for the associated modulation spaces. This is done by showing that finite linear combinations of shifts and dilates of a single function with sufficient decay...... in both direct and frequency space can constitute a frame for Triebel-Lizorkin type spaces and the associated modulation spaces. First, we extend the machinery of almost diagonal matrices to Triebel-Lizorkin type spaces and the associated modulation spaces. Next, we prove that two function systems which...... are sufficiently close have an almost diagonal “change of frame coefficient” matrix. Finally, we approximate to an arbitrary degree an already known frame for Triebel-Lizorkin type spaces and the associated modulation spaces with a single function with sufficient decay in both direct and frequency space....

  16. Effects of Peer Mediated Instruction on the Oral Reading Fluency Skills of High School Aged Struggling Readers

    Science.gov (United States)

    Josephs, Nikki L.; Jolivette, Kristine

    2016-01-01

    This study examined the effects of peer-mediated oral reading fluency instruction and narrative texts on the reading fluency and comprehension skills of adolescent struggling readers in an alternative high school setting over a period of 10 weeks. The results of this study indicate that peer-mediated repeated reading appears to be the most…

  17. High Performance Commercial Fenestration Framing Systems

    Energy Technology Data Exchange (ETDEWEB)

    Mike Manteghi; Sneh Kumar; Joshua Early; Bhaskar Adusumalli

    2010-01-31

    A major objective of the U.S. Department of Energy is to have a zero energy commercial building by the year 2025. Windows have a major influence on the energy performance of the building envelope as they control over 55% of building energy load, and represent one important area where technologies can be developed to save energy. Aluminum framing systems are used in over 80% of commercial fenestration products (i.e. windows, curtain walls, store fronts, etc.). Aluminum framing systems are often required in commercial buildings because of their inherent good structural properties and long service life, which is required from commercial and architectural frames. At the same time, they are lightweight and durable, requiring very little maintenance, and offer design flexibility. An additional benefit of aluminum framing systems is their relatively low cost and easy manufacturability. Aluminum, being an easily recyclable material, also offers sustainable features. However, from energy efficiency point of view, aluminum frames have lower thermal performance due to the very high thermal conductivity of aluminum. Fenestration systems constructed of aluminum alloys therefore have lower performance in terms of being effective barrier to energy transfer (heat loss or gain). Despite the lower energy performance, aluminum is the choice material for commercial framing systems and dominates the commercial/architectural fenestration market because of the reasons mentioned above. In addition, there is no other cost effective and energy efficient replacement material available to take place of aluminum in the commercial/architectural market. Hence it is imperative to improve the performance of aluminum framing system to improve the energy performance of commercial fenestration system and in turn reduce the energy consumption of commercial building and achieve zero energy building by 2025. The objective of this project was to develop high performance, energy efficient commercial

  18. General frame structures on quantum principal bundles

    CERN Document Server

    Durdevic, M

    1996-01-01

    A noncommutative-geometric generalization of the classical formalism of frame bundles is developed, incorporating into the theory of quantum principal bundles the concept of the Levi-Civita connection. The construction of a natural differential calculus on quantum principal frame bundles is presented, including the construction of the associated differential calculus on the structure group. General torsion operators are defined and analyzed. Illustrative examples are presented.

  19. Frame Narratives and The Gothic Subject

    OpenAIRE

    Southward, D.

    2015-01-01

    So endemic to the early Gothic, yet so often critically neglected, the trope of the found manuscript, and by extension the frame narrative, is nonetheless revealing of a particular anxiety within the genre as a whole, and one which contemporary Gothic texts increasingly problematize as a force of narrative antagonism. This paper explores the relationship between the framer and framed in both Mary Shelley's classic Gothic text Frankenstein and Richard Flanagan's Tazmanian Gothic text Gould's B...

  20. Moving frames for heart fiber geometry

    DEFF Research Database (Denmark)

    Piuze, Emmanuel; Sporring, Jon; Siddiqi, Kaleem

    2013-01-01

    frames to study the rotations of a frame field attached to the local fiber direction. This approach provides a new set of parameters that are complimentary to those of Savadjiev et al. and offers a framework for developing new models of the cardiac fiber architecture. This framework is used to compute......, the homeoid, constrains fibers to lie locally within ellipsoidal shells and yields improved fits in the rat, the dog and the human to those obtained using generalized helicoids....