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Sample records for alpha-toxin encoding plc

  1. Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens

    DEFF Research Database (Denmark)

    Abildgaard, L; Engberg, RM; Pedersen, Karl;

    2009-01-01

    The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in a-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio...... different a-toxin sequence types among the 60 strains. Moreover, a type II intron of 834 non-coding nucleotides was identified in the pic gene of three of the investigated strains. The in vitro alpha-toxin production investigated in 45 of the strains, including the three harbouring the intron, revealed no...... correlation between PFGE type, alpha-toxin sequence type, health status of the host chickens and level of a-toxin production. It is therefore concluded that neither pic gene type nor alpha-toxin production level seems to correlate to origin (healthy or diseased chicken) of the C perfringens strains. (C) 2008...

  2. Phospholipase C Produced by Clostridium botulinum Types C and D:Comparison of Gene, Enzymatic, and Biological Activities with Those of Clostridium perfringens Alpha-toxin

    Directory of Open Access Journals (Sweden)

    Sakurai,Jun

    2013-02-01

    Full Text Available Clostridium botulinum type C and D strains recently have been found to produce PLC on egg yolk agar plates. To characterize the gene, enzymatic and biological activities of C. botulinum PLCs (Cb-PLCs, the cb-plc genes from 8 strains were sequenced, and 1 representative gene was cloned and expressed as a recombinant protein. The enzymatic and hemolytic activities of the recombinant Cb-PLC were measured and compared with those of the Clostridium perfringens alpha-toxin. Each of the eight cb-plc genes encoded a 399 amino acid residue protein preceded by a 27 residue signal peptide. The protein consists of 2 domains, the N- and C-domains, and the overall amino acid sequence identity between Cb-PLC and alpha-toxin was greater than 50%, suggesting that Cb-PLC is homologous to the alpha-toxin. The key residues in the N-domain were conserved, whereas those in the C-domain which are important in membrane interaction were different than in the alpha-toxin. As expected, Cb-PLC could hydrolyze egg yolk phospholipid, p-nitrophenylphosphorylcholine, and sphingomyelin, and also exhibited hemolytic activity;however, its activities were about 4- to over 200-fold lower than those of alpha-toxin. Although Cb-PLC showed weak enzymatic and biological activities, it is speculated that Cb-PLC might play a role in the pathogenicity of botulism or for bacterial survival.

  3. Cloning and expression of AtPLC6, a gene encoding a phosphatidylinositol-specific phospholipase C in Arabidopsis thaliana

    Institute of Scientific and Technical Information of China (English)

    XU Xiaojing; CAO Zhixiang; LIU Guoqin; Madan K. Bhattacharrya; REN Dongtao

    2004-01-01

    A full-length eDNA clone corresponding to a putative phosphatidylinositol-specific phospholipase C (PIPLC) was isolated from Arabidopsis thaliana by screening a cDNA library and using RT-PCR strategy. The cDNA, designated AtPLC6, encodes a putative polypeptide of 578 amino acid residues with a calculated molecular mass of 66251.84 D and a pI of 7.24. The sequence analysis indicates that the polypeptide contains X, Y, EF-hand and C2 domains. The overall structure of putative AtPLC6 protein, like other plant PI-PLCs, is most similar to that of mammalian PLC& The recombinant AtPLC6 protein expressed in E. coli was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (IP3) and 1,2-diacylglycerol (DAG). The protein hydrolyzes PIP2 in a Ca2+-dependent manner and the optimum concentration of Ca2+ is 10 μmol/L.These results suggested that AtPLC6 gene encodes a genuine PI-PLC. Northern blot analysis showed that the AtPLC6 gene is expressed at low level in all examined tissues, such as roots,stems, leaves, flowers, siliques and seedlings under normal growth conditions. The gene is strongly induced under low temperature and weakly induced under various stresses,such as ABA, high-salt stress and heat. These results suggested that AtPLC6 might be involved in the signal-transduction pathways of cold responses of the plants.

  4. Veal calves produce less antibodies against C. perfringens alpha toxin compared to beef calves

    OpenAIRE

    Valgaeren, Bonnie R.; Bart Pardon; Evy Goossens; Stefanie Verherstraeten; Sophie Roelandt; Leen Timbermont; Nicky Van Der Vekens; Sabrina Stuyvaert; Linde Gille; Laura Van Driessche; Freddy Haesebrouck; Richard Ducatelle; Filip Van Immerseel; Piet Deprez

    2015-01-01

    Enterotoxaemia is a disease with a high associated mortality rate, affecting beef and veal calves worldwide, caused by C. perfringens alpha toxin and perfringolysin. A longitudinal study was conducted to determine the dynamics of antibodies against these toxins in 528 calves on 4 beef and 15 veal farms. The second study aimed to determine the effect of solid feed intake on the production of antibodies against alpha toxin and perfringolysin. The control group only received milk replacer, wher...

  5. Molecular cloning of Clostridium septicum vaccine strain alpha toxin gene in E. coli

    OpenAIRE

    Bozorgkhoo; Pilehchian Langroudi; Khaki; Jabbari; Moradi Bidhendi; Moosawi Shoshtari

    2014-01-01

    Clostridium septicum a Gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. C. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. The aim of the present study was to molecular cloning and sequencing of C. septicum vaccine strain alpha toxin gene. Genomic DNA was extracted using standard phenol and chloroform extraction method, and the target gene was amplified through PCR by specific primers. Quality and ...

  6. Veal Calves Produce Less Antibodies against C. Perfringens Alpha Toxin Compared to Beef Calves.

    Science.gov (United States)

    Valgaeren, Bonnie R; Pardon, Bart; Goossens, Evy; Verherstraeten, Stefanie; Roelandt, Sophie; Timbermont, Leen; Van Der Vekens, Nicky; Stuyvaert, Sabrina; Gille, Linde; Van Driessche, Laura; Haesebrouck, Freddy; Ducatelle, Richard; Van Immerseel, Filip; Deprez, Piet

    2015-07-01

    Enterotoxaemia is a disease with a high associated mortality rate, affecting beef and veal calves worldwide, caused by C. perfringens alpha toxin and perfringolysin. A longitudinal study was conducted to determine the dynamics of antibodies against these toxins in 528 calves on 4 beef and 15 veal farms. The second study aimed to determine the effect of solid feed intake on the production of antibodies against alpha toxin and perfringolysin. The control group only received milk replacer, whereas in the test group solid feed was provided. Maternal antibodies for alpha toxin were present in 45% of the veal calves and 66% of the beef calves. In beef calves a fluent transition from maternal to active immunity was observed for alpha toxin, whereas almost no veal calves developed active immunity. Perfringolysin antibodies significantly declined both in veal and beef calves. In the second study all calves were seropositive for alpha toxin throughout the experiment and solid feed intake did not alter the dynamics of alpha and perfringolysin antibodies. In conclusion, the present study showed that veal calves on a traditional milk replacer diet had significantly lower alpha toxin antibodies compared to beef calves in the risk period for enterotoxaemia, whereas no differences were noticed for perfringolysin. PMID:26184311

  7. Veal Calves Produce Less Antibodies against C. Perfringens Alpha Toxin Compared to Beef Calves

    Directory of Open Access Journals (Sweden)

    Bonnie R. Valgaeren

    2015-07-01

    Full Text Available Enterotoxaemia is a disease with a high associated mortality rate, affecting beef and veal calves worldwide, caused by C. perfringens alpha toxin and perfringolysin. A longitudinal study was conducted to determine the dynamics of antibodies against these toxins in 528 calves on 4 beef and 15 veal farms. The second study aimed to determine the effect of solid feed intake on the production of antibodies against alpha toxin and perfringolysin. The control group only received milk replacer, whereas in the test group solid feed was provided. Maternal antibodies for alpha toxin were present in 45% of the veal calves and 66% of the beef calves. In beef calves a fluent transition from maternal to active immunity was observed for alpha toxin, whereas almost no veal calves developed active immunity. Perfringolysin antibodies significantly declined both in veal and beef calves. In the second study all calves were seropositive for alpha toxin throughout the experiment and solid feed intake did not alter the dynamics of alpha and perfringolysin antibodies. In conclusion, the present study showed that veal calves on a traditional milk replacer diet had significantly lower alpha toxin antibodies compared to beef calves in the risk period for enterotoxaemia, whereas no differences were noticed for perfringolysin.

  8. The Staphylococcus aureus Alpha-Toxin Perturbs the Barrier Function in Caco-2 Epithelial Cell Monolayers by Altering Junctional Integrity

    OpenAIRE

    Kwak, Young-Keun; Vikström, Elena; Magnusson, Karl-Eric; Vécsey-Semjén, Beatrix; Colque-Navarro, Patricia; Möllby, Roland

    2012-01-01

    Increased microvascular permeability is a hallmark of sepsis and septic shock. Intestinal mucosal dysfunction may allow translocation of bacteria and their products, thereby promoting sepsis and inflammation. Although Staphylococcus aureus alpha-toxin significantly contributes to sepsis and perturbs the endothelial barrier function, little is known about possible effects of S. aureus alpha-toxin on human epithelial barrier functions. We hypothesize that S. aureus alpha-toxin in the blood can ...

  9. Passive immunization with antiserum to a nontoxic alpha-toxin mutant from Staphylococcus aureus is protective in a murine model.

    OpenAIRE

    Menzies, B E; Kernodle, D S

    1996-01-01

    A nonhemolytic, nonlethal variant of Staphylococcus aureus alpha-toxin constructed via oligonucleotide-directed mutagenesis and containing a single amino acid substitution (H-35 to L) was used to immunize a rabbit. The resulting antiserum was cross-reactive with wild-type alpha-toxin and neutralized its hemolytic activity in vitro. Passive immunization of mice with rabbit antiserum conferred protection against lethal challenge with wild-type alpha-toxin and against acute lethal challenge with...

  10. Crystallization and preliminary X-ray diffraction analysis of the complex between a human anti-alpha toxin antibody fragment and alpha toxin

    International Nuclear Information System (INIS)

    Crystals of the complex between the Fab fragment of a human anti-alpha toxin antibody and alpha toxin have been obtained. Diffraction data sets were collected to 2.56 Å resolution. Staphylococcus aureus alpha toxin (AT) has been crystallized in complex with the Fab fragment of a human antibody (MEDI4893). This constitutes the first reported crystals of AT bound to an antibody. The monoclinic crystals belonged to space group P21, with unit-cell parameters a = 85.52, b = 148.50, c = 93.82 Å, β = 99.82°. The diffraction of the crystals extended to 2.56 Å resolution. The asymmetric unit contained two MEDI4893 Fab–AT complexes. This corresponds to a crystal volume per protein weight (VM) of 2.3 Å3 Da−1 and a solvent content of 47%. The three-dimensional structure of this complex will contribute to an understanding of the molecular basis of the interaction of MEDI4893 with AT. It will also shed light on the mechanism of action of this antibody, the current evaluation of which in the field of S. aureus-mediated diseases makes it a particularly interesting case study. Finally, this study will provide the three-dimensional structure of AT in a monomeric state for the first time

  11. Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification

    OpenAIRE

    B. Radhika; N. Vinod Kumar; Sreenivasulu, D.

    2016-01-01

    Aim: The loop mediated isothermal amplification (LAMP) was standardized for rapid detection of Clostridium perfringens. Materials and Methods: A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of C. perfringens cpa gene by LAMP. The specificity of the LAMP amplified products was tested by digesting with restriction enzyme XmnI for alpha toxin gene. Results: Out of 120 samples screened 112 (93.3%) samples were positive by both LAMP and polym...

  12. Excitatory effect of Clostridium perfringens alpha toxin on the rat isolated aorta.

    OpenAIRE

    Fujii, Y.; Nomura, S; Oshita, Y.; Sakurai, J

    1986-01-01

    Clostridium perfringens alpha toxin caused contraction of the isolated aorta of the rat in a dose-dependent manner. The contractile action caused by the toxin was inhibited or abolished by calcium antagonists such as nifedipine, verapamil and cinnarizine, or a Ca-free medium, but was not affected by phentolamine, chlorpheniramine, atropine, tetrodotoxin or a low Na medium. The toxin stimulated Ca uptake into the aorta in a dose-dependent manner. 8-N,N'-diethylaminooctyl-3,4,5-trimethoxybenzoa...

  13. Induction of muscle-relaxing factor by staphylococcal alpha-toxin.

    OpenAIRE

    Harshman, S; Sugg, N

    1994-01-01

    Brain tissue and serum from mice intracerebrally injected with 1 microgram of staphylococcal alpha-toxin contained elevated amounts of a naturally occurring brain tissue component(s) called muscle-relaxing factor (MRF). MRF induced reversible, generalized, flaccid paralysis of mice after intracerebral but not intraperitoneal or intravenous administration. MRF (i) was soluble in Hanks balanced salt solution and in acidified (pH 2) Hanks balanced salt solution, in which it partitions into ethyl...

  14. Effect of staphylococcal alpha-toxin on intracellular Ca2+ in polymorphonuclear leukocytes.

    OpenAIRE

    Suttorp, N; Habben, E

    1988-01-01

    Staphylococcal alpha-toxin, a channel-forming protein, stimulates leukotriene B4 formation in rabbit polymorphonuclear leukocytes (PMN) (N. Suttorp, W. Seeger, J. Zucker-Reimann, L. Roka, and S. Bhakdi, Infect. Immun. 55:104-110, 1987). The concept was advanced that transmembrane toxin pores act as Ca2+ gates allowing passive Ca2+ influx into the cell, thus initiating stimulus response coupling. A critical step in this hypothesis is the demonstration of an increase in the cytosolic free Ca2+ ...

  15. Immunization with recombinant alpha toxin partially protects broiler chicks against experimental challenge with Clostridium perfringens.

    Science.gov (United States)

    Cooper, K K; Trinh, H T; Songer, J Glenn

    2009-01-01

    Necrotic enteritis (NE) in poultry has re-emerged as a concern for poultry producers, due in part to banning, by many countries, of the use of antimicrobial growth promoters in feeds. This re-emergence has led to a search for alternative methods for control of the disease, particularly vaccination. The objective of this work was to determine if vaccination of broiler chicks with recombinant alpha toxin protected against experimental challenge. Broiler chicks were vaccinated subcutaneously at 5 and 15 days of age, followed 10 days later by challenge with Clostridium perfringens. Birds were challenged twice daily on 4 consecutive days by mixing C. perfringens cultures with feed (three parts culture: four parts feed). Non-vaccinated birds challenged with C. perfringens developed NE at the rate of 87.8%, while only 54.9% of vaccinated birds developed lesions. In addition, non-vaccinated birds had lesion scores averaging 2.37, while average scores in vaccinated birds were 1.35. Vaccination produced an antibody response, with post-vaccination anti-alpha toxin IgG (IgY) titers in vaccinated birds more than 5-fold greater than in non-vaccinated birds. After challenge, vaccinated birds had average IgG (IgY) titers>15-fold higher than those in non-vaccinated birds. These results suggest that alpha toxin may serve as an effective immunogen, and, as such, may play a role in pathogenesis. PMID:18635321

  16. In silico, in vitro and in vivo analysis of binding affinity between N and C-domains of Clostridium perfringens alpha toxin.

    Directory of Open Access Journals (Sweden)

    Siva Ramakrishna Uppalapati

    Full Text Available Clostridium perfringens alpha toxin/phospholipase C (CP-PLC is one of the most potent bacterial toxins known to cause soft tissue infections like gas gangrene in humans and animals. It is the first bacterial toxin demonstrated to be an enzyme with phospholipase, sphingomyelinase and lecithinase activities. The toxin is comprised of an enzymatic N-domain and a binding C-domain interconnected by a flexible linker. The N-domain alone is non-toxic to mammalian cells, but incubation with C-domain restores the toxicity, the mechanism of which is still not elucidated. The objectives of the current study were to investigate the formation of a stable N and C-domain complex, to determine possible interactions between the two domains in silico and to characterize the in vitro and in vivo correlates of the interaction. To establish the existence of a stable N and C-domain hybrid, in vitro pull down assay and dot-Far Western blotting assays were employed, where it was clearly revealed that the two domains bound to each other to form an intermediate. Using bioinformatics tools like MetaPPISP, PatchDock and FireDock, we predicted that the two domains may interact with each other through electrostatic interactions between at least six pairs of amino acids. This N and C-domains interacted with each other in 1:1 ratio and the hybrid lysed mouse erythrocytes in a slower kinetics when compared with wild type native Cp-PLC. BALB/c mice when challenged with N and C-domain hybrid demonstrated severe myonecrosis at the site of injection while no death was observed. Our results provide further insight into better understanding the mechanism for the toxicity of Cp-PLC N and C-domain mixture.

  17. Osmoprotectant-dependent expression of plcH, encoding the hemolytic phospholipase C, is subject to novel catabolite repression control in Pseudomonas aeruginosa PAO1.

    OpenAIRE

    Sage, A E; Vasil, M L

    1997-01-01

    Expression of the hemolytic phospholipase C (PlcH) of Pseudomonas aeruginosa is induced under phosphate starvation conditions or in the presence of the osmoprotectants choline and glycine betaine. Because choline and glycine betaine may serve as carbon and energy sources in addition to conferring osmoprotection to P. aeruginosa, it seemed possible that induction of plcH is subject to catabolite repression control (CRC) by tricarboxylic cycle intermediates such as succinate. Total phospholipas...

  18. Solucions PLC obertes

    OpenAIRE

    López Llauradó, Oriol

    2006-01-01

    Treball que, després de revisar el concepte de PLC i d'exposar la necessitat de solucions obertes, defineix el concepte de PLC obert, basat en programari lliure i presenta alguns projectes interessants de PLC obert.

  19. Detection of Clostridium perfringens alpha toxin gene in lambs by loop mediated isothermal amplification

    Directory of Open Access Journals (Sweden)

    B. Radhika

    2016-01-01

    Full Text Available Aim: The loop mediated isothermal amplification (LAMP was standardized for rapid detection of Clostridium perfringens. Materials and Methods: A total of 120 fecal samples were collected from enterotoxemia suspected lambs were used for screening of C. perfringens cpa gene by LAMP. The specificity of the LAMP amplified products was tested by digesting with restriction enzyme XmnI for alpha toxin gene. Results: Out of 120 samples screened 112 (93.3% samples were positive by both LAMP and polymerase chain reaction (PCR for detection of cpa gene which indicated the equal sensitivity of both the tests. The enzyme produced single cut in 162 base pair amplified product of alpha toxin gene at 81 base pair resulting in a single band in gel electrophoresis. Conclusion: Both LAMP and PCR for detection of cpa gene indicated the equal sensitivity of both the tests. Standardization of LAMP reaction for amplification of epsilon and beta toxin genes will help to identify the C. perfringens toxin types from the clinical samples. The test could be a suitable alternative to the PCR in detection of toxin types without the help of sophisticated machinery like thermal cycler. Considering its simplicity in operation and high sensitivity, there is the potential use of this technique in clinical diagnosis and surveillance of infectious diseases.

  20. Identification and validation of a linear protective neutralizing epitope in the β-pore domain of alpha toxin.

    Directory of Open Access Journals (Sweden)

    Jon Oscherwitz

    Full Text Available The plethora of virulence factors associated with Staphylococcus aureus make this bacterium an attractive candidate for a molecularly-designed epitope-focused vaccine. This approach, which necessitates the identification of neutralizing epitopes for incorporation into a vaccine construct, is being evaluated for pathogens where conventional approaches have failed to elicit protective humoral responses, like HIV-1 and malaria, but may also hold promise for pathogens like S. aureus, where the elicitation of humoral immunity against multiple virulence factors may be required for development of an effective vaccine. Among the virulence factors employed by S. aureus, animal model and epidemiological data suggest that alpha toxin, a multimeric β-pore forming toxin like protective antigen from Bacillus anthracis, is particularly critical, yet no candidate neutralizing epitopes have been delineated in alpha toxin to date. We have previously shown that a linear determinant in the 2β2-2β3 loop of the pore forming domain of B. anthracis protective antigen is a linear neutralizing epitope. Antibody against this site is highly potent for neutralizing anthrax lethal toxin in vitro and for protection of rabbits in vivo from virulent B. anthracis. We hypothesized that sequences in the β-pore of S. aureus alpha toxin that share structural and functional homology to β-pore sequences in protective antigen would contain a similarly critical neutralizing epitope. Using an in vivo mapping strategy employing peptide immunogens, an optimized in vitro toxin neutralization assay, and an in vivo dermonecrosis model, we have now confirmed the presence of this epitope in alpha toxin, termed the pore neutralizing determinant. Antibody specific for this determinant neutralizes alpha toxin in vitro, and is highly effective for mitigating dermonecrosis and bacterial growth in a mouse model of S. aureus USA300 skin infection. The delineation of this linear neutralizing

  1. The effect of feeding a commercial essential oil product on Clostridium perfringens numbers in the intestine of broiler chickens measured by real-time PCR targeting the α-toxin-encoding gene (plc)

    DEFF Research Database (Denmark)

    Abildgaard, Lone; Højberg, Ole; Schramm, Andreas;

    2010-01-01

    Proliferation of Clostridium perfringens type A in the broiler intestinal tract is related to poor growth and litter quality, and can under certain conditions lead to the development of necrotic enteritis (NE), a severe gastrointestinal disease in broilers. The aim of the present study was to inv...... quantification of C. perfringens type A in broilers, a real-time PCR assay, targeting the α-toxin-encoding plc gene, was developed for use in ileal and caecal samples and was shown to be a fast and reliable alternative to conventional plate counting....

  2. Transacsys PLC - Final Results

    CERN Multimedia

    2002-01-01

    Final results from Transacsys PLC. A subsidary of this company was set up to develop the CERN EDH system into a commercial product but incurred too much financial loss so the project was cancelled (1/2 page).

  3. Clostridium perfringens strains from bovine enterotoxemia cases are not superior in in vitro production of alpha toxin, perfringolysin O and proteolytic enzymes

    OpenAIRE

    Goossens, Evy; Verherstraeten, Stefanie; Timbermont, Leen; Valgaeren, Bonnie; Pardon, Bart; Haesebrouck, Freddy; Ducatelle, Richard; Deprez, Piet; Van Immerseel, Filip

    2014-01-01

    Background: Bovine enterotoxemia is a major cause of mortality in veal calves. Predominantly veal calves of beef cattle breeds are affected and losses due to enterotoxemia may account for up to 20% of total mortality. Clostridium perfringens type A is considered to be the causative agent. Recently, alpha toxin and perfringolysin O have been proposed to play an essential role in the development of disease. However, other potential virulence factors also may play a role in the pathogenesis of b...

  4. The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth.

    OpenAIRE

    Yoko-o, T; Matsui, Y; Yagisawa, H; Nojima, H; Uno, I; Toh-E, A

    1993-01-01

    Using the polymerase chain reaction technique, we have isolated a gene that encodes a putative phosphoinositide-specific phospholipase C (PLC) in the yeast Saccharomyces cerevisiae. The nucleotide sequence indicates that the gene encodes a polypeptide of 869 amino acid residues with a calculated molecular mass of 101 kDa. This polypeptide has both the X and Y regions conserved among mammalian PLC-beta, -gamma, and -delta, and the structure is most similar to that of mammalian PLC-delta. This ...

  5. The Parametric model for PLC reference chanells and its verification in real PLC environment

    OpenAIRE

    Róka, R.

    2008-01-01

    For the expansion of PLC systems, it is necesssary to have a detailed knowledge of the PLC transmission channel properties. This contribution shortly discusses characteristics of the PLC environment and a classification of PLC transmission channels. A main part is focused on the parametric model for PLC reference channels and its verification in the real PLC environment utilizing experimental measurements.

  6. The Parametric Model for PLC Reference Chanells and its Verification in Real PLC Environment

    OpenAIRE

    Rastislav Roka

    2008-01-01

    For the expansion of PLC systems, it is necesssary to have a detailed knowledge of the PLC transmission channel properties. This contribution shortly discusses characteristics of the PLC environment and a classification of PLC transmission channels. A main part is focused on the parametric model for PLC reference channels and its verification in the real PLC environment utilizing experimental measurements.

  7. Reliability analysis of PLC safety equipment

    International Nuclear Information System (INIS)

    FMEA analysis for Nuclear Safety Grade PLC, failure rate prediction for nuclear safety grade PLC, sensitivity analysis for components failure rate of nuclear safety grade PLC, unavailability analysis support for nuclear safety system

  8. Slovak Electric, plc

    International Nuclear Information System (INIS)

    In this book the structure and activities of the Slovenske Elektrarne, a.s. (Slovak Electric, plc.) are presented. The parameters of the Bohunice NPPs as well as Mochovce NPP are described in detail. Other power plant (coal fired power plants and hydroelectric power plants as well as power transmission and distribution systems in the Slovak Republic are described too. Treatment of radioactive wastes are performed in the the Bohunice Radwaste Treatment Centre (BSC RAO) and radioactive waste are storages in Mochovce National Radioactive Waste Repository for the Slovak Republic

  9. Equity valuation Asos PLC

    OpenAIRE

    Marques, Mariana Borges

    2015-01-01

    This thesis aims to study the value of Asos PLC. The valuation was based in four methodologies, the Discounted Cash Flow (DCF), the Economic Value Added (EVA), the Trading Multiples and the Transaction Multiples. Using the DCF method, a price of 3.054p was obtained and the EVA yielded a comparable lower value of 2.528p. Regarding the Relative Valuation, this was the method which delivered the lowest values of 1.573p for the Trading Multiples and 1.253p for the Transaction Multiples. It ...

  10. The Futility of PLC Lite

    Science.gov (United States)

    DuFour, Rick; Reeves, Douglas

    2016-01-01

    Although many schools around the world have claimed to embrace the Professional Learning Community process, it would be more accurate to describe the current state of affairs in many schools as PLC Lite. Educators rename their traditional faculty or department meetings as PLC meetings, engage in book studies that result in no action, or devote…

  11. Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity.

    Science.gov (United States)

    Abd-El-Haliem, Ahmed M; Vossen, Jack H; van Zeijl, Arjan; Dezhsetan, Sara; Testerink, Christa; Seidl, Michael F; Beck, Martina; Strutt, James; Robatzek, Silke; Joosten, Matthieu H A J

    2016-09-01

    Plants possess effective mechanisms to quickly respond to biotic and abiotic stresses. The rapid activation of phosphatidylinositol-specific phospholipase C (PLC) enzymes occurs early after the stimulation of plant immune-receptors. Genomes of different plant species encode multiple PLC homologs belonging to one class, PLCζ. Here we determined whether all tomato homologs encode active enzymes and whether they can generate signals that are distinct from one another. We searched the recently completed tomato (Solanum lycopersicum) genome sequence and identified a total of seven PLCs. Recombinant proteins were produced for all tomato PLCs, except for SlPLC7. The purified proteins showed typical PLC activity, as different PLC substrates were hydrolysed to produce diacylglycerol. We studied SlPLC2, SlPLC4 and SlPLC5 enzymes in more detail and observed distinct requirements for Ca(2+) ions and pH, for both their optimum activity and substrate preference. This indicates that each enzyme could be differentially and specifically regulated in vivo, leading to the generation of PLC homolog-specific signals in response to different stimuli. PLC overexpression and specific inhibition of PLC activity revealed that PLC is required for both specific effector- and more general "pattern"-triggered immunity. For the latter, we found that both the flagellin-triggered response and the internalization of the corresponding receptor, Flagellin Sensing 2 (FLS2) of Arabidopsis thaliana, are suppressed by inhibition of PLC activity. Altogether, our data support an important role for PLC enzymes in plant defence signalling downstream of immune receptors. This article is part of a Special Issue entitled: Plant Lipid Biology edited by Kent D. Chapman and Ivo Feussner. PMID:26825689

  12. Design of object control using PLC

    OpenAIRE

    Ryčl, Jakub

    2013-01-01

    The work deals with the issue of control system using PLC. In the introduction are processed mainly general information about PLC and ways of his programming. Next is prepared methodology for system design with PLC, which is then tested in the practical part. It describes in detail the complete formation of the hall ventilation system with control by PLC.

  13. Development of PLC Technology in China

    Institute of Scientific and Technical Information of China (English)

    Liu Jianming; Cuiwei

    2005-01-01

    In China there built a large scale of power line communication (Abbr. PLC)testing network. This paper presents the research and development on PLC technology in China and also the application and popularization of PLC in Chinese market. At the end, the next focal points of PLC technology research are deeply discussed.

  14. Slovak Electric, Plc., 1997

    International Nuclear Information System (INIS)

    Slovenske elektrarne, a.s. (Slovak Electric, Plc.) was established on November 1, 1994 as one entity among new entities created as successors to the former Slovensky energeticky podnik. The subject activity is the generation of electric power, operation of transmission 220 kV and 400 kV systems, transit, import, export, and sales of electric power. Besides these activities the company deals with generation, distribution, and sales of heat. The company operates one nuclear power station, three thermal power plants, and thirty hydro power plants. One nuclear Power plant is under construction (state up tu June 1997). On this CD ROM next chapters are presented: (1) The structure of the company; (2) The production Units; (3) The economic power of the company; (4) The operation culture of the company; (5) The strategic plans of the company

  15. Slovak Electric, Plc., 1998

    International Nuclear Information System (INIS)

    Slovenske elektrarne, a.s. (Slovak Electric, Plc., abbrevation 'SE, a.s.') is the Slovak electricity generating utility, incorporated on November 1, 1994 as one of new companies formed from substantially all of the assets and a legal successor of Slovensky energeticky podnik, s.p., founded on January 1, 1969 in the form of SEP group. From its predecessor, Slovak Electric, Plc., took over generation of power, operation of 220 kV and 400 kV power system, transit, import, export, and sale of electricity. It is also involved in generation, distribution, and sale of heat. At present, SE's share of electriciry sales in the Slovak Republic is 88.47%. Electricity is delivered to three regional distribution companies and directly to several major industrial enterprises. SE, a.s. operates one nuclear power station, three thermal power plants, and 30 hydro power plants. The second nuclear power plant is under construction (state up tu June 1997) and SE is participating in the construction of two hydro power plants and one combined cycle power plant. The efforts of SE, s.a. focus on the generation of power and heat with minimal environmental impacts. Ecology is given priority in the SE, a.s. development programmes. SE's mission is to permanently satisfy customers' needs, for an acceptable price and with minimal environmental impact. On this CD ROM next chapters are presented: (1) The structure of the Company; (2) Production units; (3) The economic power of the Company; (4) The operation culture; (5) The strategic plans of the Company

  16. Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity

    NARCIS (Netherlands)

    A.M. Abd-el-Haliem; J.H. Vossen; A. van Zeijl; S. Dezhsetan; C. Testerink; M.F. Seidl; M. Beck; J. Strutt; S. Robatzek; M.H.A.J. Joosten

    2016-01-01

    Plants possess effective mechanisms to quickly respond to biotic and abiotic stresses. The rapid activation of phosphatidylinositol-specific phospholipase C (PLC) enzymes occurs early after the stimulation of plant immune-receptors. Genomes of different plant species encode multiple PLC homologs bel

  17. Evaluation in broilers of the probiotic properties of Pichia pastoris and a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene.

    Science.gov (United States)

    Gil de los Santos, João Rodrigo; Storch, Otávio Brod; Fernandes, Cristina Gevehr; Gil-Turnes, Carlos

    2012-05-01

    The probiotic properties of Pichia pastoris and of a recombinant P. pastoris containing the Clostridium perfringens alpha toxin gene were evaluated in broilers. One-day-old chicks randomly divided in four groups were fed with commercial feed devoid of antibacterials. The control group (1) received plain food, while the other groups were supplemented with either P. pastoris (2), the recombinant P. pastoris (3) or Bacillus cereus var. Toyoi (4). At day 49, live weights, feed efficiency and seroconversions were higher (P0.05) among the supplemented groups. Adverse reactions were not observed in histopathologic evaluation. We concluded that P. pastoris and the recombinant P. pastoris could be used as probiotics in broilers. PMID:22176763

  18. PLC VE PLC ILE ROTORU SARGILI ASENKRON MOTORA YOL VERME

    OpenAIRE

    İSMAİL COŞKUN; İBRAHİM YÜCEDAĞ

    1998-01-01

    Günilinüzde tcknoloj i hızla ilerlemekte ve bw1a paralel olarak kullanılan sistcnıler de değişmektedir. Bu değişim tasarıını kolay. ckonoınik. luzlı tepkili sistemi elde etıneye yönelik alınaktadır. Değişim zinciri içerisinde Otoınas\\on ve Otoınatik Kumanda sektörü önemli bir halka� ı teşkil etıncktcdir. Bu sektörde, Programlanabilir Mantık Kontrol ( PLC ) hızla kullanılmaya başlanmıştır. Bu çalışınada PLC' ·ı cr genel olarak değerlendirilmiş olup. PLC ilc rotoru sargılı asenkron ınotora yol ...

  19. Equity valuation of Tesco Plc.

    OpenAIRE

    Schmitz, Clemens

    2015-01-01

    This dissertation aimed to value the British retailer Tesco Plc. The objective was to determine a target price for the company and as a consequence determine a buy or sell recommendation when comparing it to the current market price. After the state of the art of literature regarding equity valuation has been assessed, the retail industry as well as Tesco have been analysed in more detail. Based on this, the retail business of Tesco has been valued using the Adjusted Present Va...

  20. A multi-omics approach identifies key hubs associated with cell type-specific responses of airway epithelial cells to staphylococcal alpha-toxin.

    Directory of Open Access Journals (Sweden)

    Erik Richter

    Full Text Available Responsiveness of cells to alpha-toxin (Hla from Staphylococcus aureus appears to occur in a cell-type dependent manner. Here, we compare two human bronchial epithelial cell lines, i.e. Hla-susceptible 16HBE14o- and Hla-resistant S9 cells, by a quantitative multi-omics strategy for a better understanding of Hla-induced cellular programs. Phosphoproteomics revealed a substantial impact on phosphorylation-dependent signaling in both cell models and highlights alterations in signaling pathways associated with cell-cell and cell-matrix contacts as well as the actin cytoskeleton as key features of early rHla-induced effects. Along comparable changes in down-stream activity of major protein kinases significant differences between both models were found upon rHla-treatment including activation of the epidermal growth factor receptor EGFR and mitogen-activated protein kinases MAPK1/3 signaling in S9 and repression in 16HBE14o- cells. System-wide transcript and protein expression profiling indicate induction of an immediate early response in either model. In addition, EGFR and MAPK1/3-mediated changes in gene expression suggest cellular recovery and survival in S9 cells but cell death in 16HBE14o- cells. Strikingly, inhibition of the EGFR sensitized S9 cells to Hla indicating that the cellular capacity of activation of the EGFR is a major protective determinant against Hla-mediated cytotoxic effects.

  1. PLC Based Hydraulic Auto Ladle System

    OpenAIRE

    Amogh Tayade; Anuja Chitre

    2014-01-01

    In this paper we have implemented a PLC based Hydraulic Auto Ladle System for Casting Department of Victory Precisions Pvt. Ltd. Chakan, Pune. This project work presents the study and design of PLC based Hydraulic Auto Ladle System. Aluminium pouring is the key process in Casting and Forging industry. Different products are manufactured by the company for automobile sector using aluminium. Programmable Logic Controller (PLC) is used for the automation of pouring process. Au...

  2. Speed Control of Induction Motor Using PLC and SCADA System

    Directory of Open Access Journals (Sweden)

    Ayman Seksak Elsaid,

    2016-01-01

    Full Text Available Automation or automatic control is the use of various control systems for operating equipment such as machinery, processes in factories, boilers and heat-treating ovens, switching in telephone networks, steering and stabilization of ships, aircraft and other applications with minimal or reduced human intervention. Some processes have been completely automated. The motor speed is controlled via the driver as an open loop control. To make a more precise closed loop control of motor speed we will use a tachometer to measure the speed and feed it back to the PLC, which compares to the desired value and take a control action, then the signal is transferred to the motor – via driver – to increase / decrease the speed. We will measure the speed of the motor using an incremental rotary encoder by adjusting parameters (PLC, driver and also we need to reduce the overall cost of the system. Our control system will be held using the available Siemens PLC. In addition, we will monitor motor parameters via SCADA system.

  3. 大豆磷脂酶C基因GmPLC12的表达分析与原核表达%Expression Analysis and Prokaryotic Expression of GmPLC12 in Glycine max

    Institute of Scientific and Technical Information of China (English)

    王法微; 包桥桥; 邓宇; 董金晔; 崔志彤; 王慧莹; 修华谦; 李海燕

    2015-01-01

    Phospholipase C (PLC) is a class of enzymes that cleave phospholipids into diacyl glycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). It plays an important role in plant abiotic stresses. In this study, we ifrstly cloned the GmPLC12 gene from soybean (Glycine max). The full-length of GmPLC12 is 1 671 bp and encoding 556 amino acids. Phylogenetic analysis indicated that GmPLC12 shared highest homology with PvPLC1 and VuPLC. Real-time quantitative analysis showed that the expression levels of GmPLC12 were induced by salt, alkali and salt-alkaline treatments, especially salt-alkaline stress. Subsequently, GmPLC12 was ligated into pE-T28a vector and transferred into Escherichia coli strain BL21. The recombinant protein was induced by IPTG and showed an about 70 kDa bands, which was consistent with prediction.%磷脂酶C (phospholipase C, PLC)是一类能够水解磷脂生成肌醇三磷酸与二酰甘油的酶,在植物非生物胁迫中起着重要的调控作用。本研究首次从大豆中克隆了大豆磷脂酶C基因GmPLC12,其全长cDNA序列1671 bp,编码一个由556个氨基酸组成的蛋白。系统发育树分析发现, GmPLC12与菜豆PvPLC1、豇豆VuPLC同源性最近。利用实时定量PCR分析发现, GmPLC12在盐、碱以及盐碱胁迫下表达量升高极为显著,尤其是盐碱胁迫。将GmPLC12基因连接到原核表达载体中并利用IPTG诱导表达,产生一条约为70 kDa的条带,与预期结果一致。

  4. Automated Formal Verification for PLC Control Systems

    CERN Multimedia

    Fernández Adiego, Borja

    2014-01-01

    Programmable Logic Controllers (PLCs) are widely used devices used in industrial control systems. Ensuring that the PLC software is compliant with its specification is a challenging task. Formal verification has become a recommended practice to ensure the correctness of the safety-critical software. However, these techniques are still not widely applied in industry due to the complexity of building formal models, which represent the system and the formalization of requirement specifications. We propose a general methodology to perform automated model checking of complex properties expressed in temporal logics (e.g. CTL, LTL) on PLC programs. This methodology is based on an Intermediate Model (IM), meant to transform PLC programs written in any of the languages described in the IEC 61131-3 standard (ST, IL, etc.) to different modeling languages of verification tools. This approach has been applied to CERN PLC programs validating the methodology.

  5. Theory of Robotics Arm Control with PLC

    OpenAIRE

    Safdar, Bilal

    2015-01-01

    This thesis was commissioned for Saimaa University of Applied Sciences with the aim of understanding the control system for a robotic arm. The Robotic Arm in this case was from Saimaa University of Applied Sciences. The main objective of this thesis was to understand a control system for the Robotic Arm using a programmable logic controller (PLC) along with gripper. The control system consisted of the electronic components (the PLC, a motor controller, a voltage regulator, a control pende...

  6. Plc Based Scrap Management System

    Directory of Open Access Journals (Sweden)

    Nidhi Mishra

    2014-03-01

    Full Text Available In this paper we have implemented an automated scrap management system for Maintenance Department of Bharat Forge Ltd. Hadapsar, Pune. Bharat Forge is a well known industry for manufacturing of Crank Shaft and various kinds of axels. To manage the scrap & wastage during mass production is a tedious and time consuming job. Scrap Management System is used for storing the scrap & garbage. This Scrap Management System had several problem like huge manpower requirement, time due to which there may be chances of misoperation due to unawareness of handling the system. When any fault is generated due to locking or mechanical operating problem of limit switches, the whole operation can be halt. These flaws lead to increasing concern regarding safety operations and constant maintenance. The conventional system which is in use was developed with a relay logic to control all its operations. But due to bulkiness of the relay logic and number of wiring there are lot of problems during maintenance of the system. This leads to develop some efficient system which find a solution to the painstaking maintenance problems of relay logic, ease of handling, reduction of manpower, & nullify the storing problem of scrap. This can be satisfactorily achieved by replacing the old relay logic by a newly designed PLC system by us.

  7. PLC technologies: present and future

    Science.gov (United States)

    Okamoto, Katsunari

    2001-07-01

    Current performances and future prospects of functional planar lightwave circuits (PLCs) will be described. Channel numbers of AWGs have been dramatically increased up to 400ch in single wafer. In the multi-chip configuration, 1010 ch has been achieved with 10 GHz channel spacing. Although throughput of the transport can be increased to tera-bit level by the WDM technology, signal processing in the electrical layer becomes a serious problem due to its sped limit. Optical functional devices, therefore, are important for solving these issues. Various kinds of optical signal processing devices have been developed; they are, dispersion slope equalizers, PMD equalizers, temporal pulse shapers and optical label recognition circuits. Optical address signal recognition will be a key technology for future label- switched networks, where each router has to provide an ultrahigh throughput exceeding the electronic speed limits. We proposed a novel optical circuit for recognizing an optical pulse pattern, which is based on an optical digital- to-analog converter fabricated on a PLC. The circuit converts the optical pulse pattern to analog optical amplitude, and enables us to recognize the address.

  8. Modem -repeater for narrow band technology of PLC

    OpenAIRE

    Bednář, Jan

    2014-01-01

    Bachelor’s thesis is focused on PLC communications, particulary on PLC modem. For starters it is necessery to learn about basics of PLC, learn how is PLC transmited, advantages and disadvantages. In the next part i will focus on modem itself, choosing a suitable communication unit by given parameters, creating external circuits to adjust the signal for our purposes. At last i will design printed circuit, realize the modem and verify its funcionality.

  9. The Setup Programmable Logical Controller (PLC) and an asynchronous engine

    OpenAIRE

    Černý, David

    2013-01-01

    The aim of this Bachelor Thesis is to design a Setup using the Programmable Logic Controller (PLC). The five essential assignments were prepared using both digital and analog PLC?s inputs and outputs. The purpose of the assignments is to teach and demonstrate the functionality of the PLC Setup.

  10. PlcR1 and PlcR2 are putative calcium-binding proteins required for secretion of the hemolytic phospholipase C of Pseudomonas aeruginosa.

    OpenAIRE

    Cota-Gomez, A; Vasil, A I; Kadurugamuwa, J; Beveridge, T J; Schweizer, H. P.; Vasil, M L

    1997-01-01

    The plcHR operon of Pseudomonas aeruginosa includes the structural gene for the hemolytic phospholipase C,plcH (previously known as plcS), and two overlapping, in-phase, genes designated plcR1 and plcR2. Hemolytic and phospholipase C (PLC) activities produced by Escherichia coli and P. aeruginosa T7 expression systems were measured in strains carrying both plcH and plcR genes and in strains carrying each gene separately. When plcH was expressed by itself in the E. coli T7 system, the area of ...

  11. Amersham International plc. Report and Accounts 1995

    International Nuclear Information System (INIS)

    The Annual Report and Accounts for Amersham International plc for the year 1995 are presented. This leading health science company develops products, services and technologies based on its expertise in labelling and detection at the molecular level. Recent work in the fields of life science research, nuclear medicine and industrial quality and safety assurance are described. (UK)

  12. A gene encoding a phosphatidylinositol-specific phospholipase C is induced by dehydration and salt stress in Arabidopsis thaliana.

    OpenAIRE

    Hirayama, T.; Ohto, C; Mizoguchi, T; Shinozaki, K

    1995-01-01

    A cDNA corresponding to a putative phosphatidylinositol-specific phospholipase C (PI-PLC) in the higher plant Arabidopsis thaliana was cloned by use of the polymerase chain reaction. The cDNA, designated cAtPLC1, encodes a putative polypeptide of 561 aa with a calculated molecular mass of 64 kDa. The putative product includes so-called X and Y domains found in all PI-PLCs identified to date. In mammalian cells, there are three types of PI-PLC, PLC-beta, -gamma, and -delta. The overall structu...

  13. PLC based Smart Street Lighting Control

    Directory of Open Access Journals (Sweden)

    D.V.Pushpa Latha

    2013-12-01

    Full Text Available Conventional street lighting systems in most of the areas are Online at regular intervals of time irrespective of the seasonal variations. The street lights are simply switched on at afternoon and turned off in the morning. The consequence is that a large amount of Power is wasted meaninglessly. As energy consumption is an issue of increasing interest, possible energy savings in public street lighting systems are recently discussed from different viewpoints. The purpose of this work is to describe the Smart Street Lighting system, an approach to accomplish the demand for flexible public lighting systems using a Programmable Logic controller (PLC. The main difference from other computers is that PLCs are armoured for severe conditions such as dust, moisture, heat, cold etc., and have the facility for extensive input/output (I/O arrangements. In the proposed paper, street lights are controlled using millennium 3 PLC taking the seasonal variations into consideration.

  14. Power Management using PLC and SCADA

    Directory of Open Access Journals (Sweden)

    Ambarish A. Salodkar

    2012-02-01

    Full Text Available Operation, control and protection system can be implemented to cover various industries starting from small scale industries to wide area operation and controlling. Power Control operation with Supervisory Control Data Acquisition (SCADA System is mandatory to run smooth heater control in industries. High scale SCADA system with enhanced features not only used for wide area system operation, but also covers Distribution Management System (DMS, Energy Management System (EMS, various Network Applications and Metering Management systems. This implementation includes power management for such industries using PLC and SCADA system. Control algorithm measures critical parameters and adjust variable outputs to optimize power by turning ON/OFF the heater with improved performance. Further electricity cost further reduces by around 5�20PLC and SCADA is used to model and realize the complete system and perform the experiment for result analysis.

  15. Intelligent traffic control system using PLC

    Science.gov (United States)

    Barz, C.; Todea, C.; Latinovic, T.; Preradovic, D. M.; Deaconu, S.; Berdie, A.

    2016-08-01

    The paper presents the traffic control system controlled through a PLC which takes the signals from different sensors on roads. The global system developed ensures the coordination of four intersections, setting a path that respects coordination type green light, the integration of additional sensors, the implementation of probes radar to inform traffic participants about recommended speed for accessing the green state located in the intersection that will follow to cross.

  16. NIH 3T3 cells stably transfected with the gene encoding phosphatidylcholine-hydrolyzing phospholipase C from Bacillus cereus acquire a transformed phenotype.

    OpenAIRE

    Johansen, T.; Bjørkøy, G; Overvatn, A; Diaz-Meco, M T; Traavik, T; Moscat, J

    1994-01-01

    In order to determine whether chronic elevation of intracellular diacylglycerol levels generated by hydrolysis of phosphatidylcholine (PC) by PC-hydrolyzing phospholipase C (PC-PLC) is oncogenic, we generated stable transfectants of NIH 3T3 cells expressing the gene encoding PC-PLC from Bacillus cereus. We found that constitutive expression of this gene (plc) led to transformation of NIH 3T3 cells as evidenced by anchorage-independent growth in soft agar, formation of transformed foci in tiss...

  17. The use of compact programmable controllers (PLC) for measuring applications

    OpenAIRE

    Burda, Tomáš

    2012-01-01

    The aim of this thesis is to determine the extent to which one can use compact PLC technology for measurement applications. The first chapter "Programmable Logic Controllers" describes the basic functions and structure of PLC. The next chapter provides a brief list of compact PLC series produced by different manufacturers which are usable for measurement applications. With the selected automat I performed some measurement and present an evaluation of the results. The last chapt...

  18. Development of tools for automatic generation of PLC code

    CERN Document Server

    Koutli, Maria; Rochez, Jacques

    This Master thesis was performed at CERN and more specifically in the EN-ICE-PLC section. The Thesis describes the integration of two PLC platforms, that are based on CODESYS development tool, to the CERN defined industrial framework, UNICOS. CODESYS is a development tool for PLC programming, based on IEC 61131-3 standard, and is adopted by many PLC manufacturers. The two PLC development environments are, the SoMachine from Schneider and the TwinCAT from Beckhoff. The two CODESYS compatible PLCs, should be controlled by the SCADA system of Siemens, WinCC OA. The framework includes a library of Function Blocks (objects) for the PLC programs and a software for automatic generation of the PLC code based on this library, called UAB. The integration aimed to give a solution that is shared by both PLC platforms and was based on the PLCOpen XML scheme. The developed tools were demonstrated by creating a control application for both PLC environments and testing of the behavior of the code of the library.

  19. 76 FR 44079 - M (2003) PLC (f/k/a Marconi PLC), Mayfair Mining & Minerals, Inc., MM2 Group, Inc., Nayna...

    Science.gov (United States)

    2011-07-22

    ... From the Federal Register Online via the Government Publishing Office SECURITIES AND EXCHANGE COMMISSION M (2003) PLC (f/k/a Marconi PLC), Mayfair Mining & Minerals, Inc.,MM2 Group, Inc., Nayna Networks... current and accurate information concerning the securities of M (2003) PLC (f/k/a Marconi PLC) because...

  20. Cascade model of power lines for PLC

    OpenAIRE

    Dziura, Michal

    2012-01-01

    This bachelor´s thesis deals with studies of power-line in terms of possibilities for using grid as a transmission medium for data transmission. Theoretical part is focused on PLC technology and the parameters of high-voltage lines. In very practical part the modeling of power-line by cascade parameters of two-port network is examined. Influence of va-rious changes in network topology are included in this thesis and also their influence on transfer function. The last part outlines the method ...

  1. Equity valuation : Rolls-Royce PLC

    OpenAIRE

    Fonseca, Mafalda Filipa Sousa

    2014-01-01

    The purpose of this thesis is to evaluate Rolls-Royce PLC. In order to do so, two valuation methodologies will be presented. The first one regards the Discount Cash Flow – WACC methodology, where each sector of the company is valued. By doing so, it is possible to reach a price per share of 1369 pence. Therefore, when using this methodology, the company is presented as a buying opportunity (the market yields a price of 1112 pence.) The second valuation presented and which is introduced ...

  2. Insertion- and Deletion-Associated Genetic Diversity of Mycobacterium tuberculosis Phospholipase C-Encoding Genes among 106 Clinical Isolates from Turkey

    OpenAIRE

    Talarico, Sarah; Durmaz, Riza; Yang, Zhenhua

    2005-01-01

    Bacterial phospholipase C has been reported to play a role in the pathogenesis of many bacteria. In order to gain a better understanding of the potential role of Mycobacterium tuberculosis phospholipase C in the pathogenesis of human tuberculosis, we investigated the genetic diversity of the four M. tuberculosis phospholipase C-encoding genes (plcA, plcB, plcC, and plcD) resulting from the IS6110 insertion and associated deletion, among 106 clinical isolates obtained from Turkey, by using PCR...

  3. PLC Based Automatic Multistoried Car Parking System

    Directory of Open Access Journals (Sweden)

    Swanand S .Vaze

    2014-12-01

    Full Text Available This project work presents the study and design of PLC based Automatic Multistoried Car Parking System. Multistoried car parking is an arrangement which is used to park a large number of vehicles in least possible place. For making this arrangement in a real plan very high technological instruments are required. In this project a prototype of such a model is made. This prototype model is made for accommodating twelve cars at a time. Availability of the space for parking is detected by optical proximity sensor which is placed on the pallet. A motor controlled elevator is used to lift the cars. Elevator status is indicated by LED which is placed on ground floor. Controlling of the platforms and checking the vacancies is done by PLC. For unparking of car, keyboard is interfaced with the model for selection of required platform. Automation is done to reduce requirement of space and also to reduce human errors, which in-turn results in highest security and greatest flexibility. Due to these advantages, this system can be used in hotels, railway stations, airports where crowding of car is more.

  4. The PlcR virulence regulon of Bacillus cereus.

    Directory of Open Access Journals (Sweden)

    Michel Gohar

    Full Text Available PlcR is a Bacillus cereus transcriptional regulator, which activates gene expression by binding to a nucleotidic sequence called the 'PlcR box'. To build a list of all genes included in the PlcR regulon, a consensus sequence was identified by directed mutagenesis. The reference strain ATCC14579 sequenced genome was searched for occurrences of this consensus sequence to produce a virtual regulon. PlcR control of these genes was confirmed by comparing gene expression in the reference strain and its isogenic Delta-plcR strain using DNA microarrays, lacZ fusions and proteomics methods. The resulting list included 45 genes controlled by 28 PlcR boxes. Forty of the PlcR controlled proteins were exported, of which 22 were secreted in the extracellular medium and 18 were bound or attached to cell wall structures (membrane or peptidoglycan layer. The functions of these proteins were related to food supply (phospholipases, proteases, toxins, cell protection (bacteriocins, toxins, transporters, cell wall biogenesis and environment-sensing (two-component sensors, chemotaxis proteins, GGDEF family regulators. Four genes coded for cytoplasmic regulators. The PlcR regulon appears to integrate a large range of environmental signals, including food deprivation and self cell-density, and regulate the transcription of genes designed to overcome obstacles that hinder B. cereus growth within the host: food supply, host barriers, host immune defenses, and competition with other bacterial species. PlcR appears to be a key component in the efficient adaptation of B. cereus to its host environment.

  5. Dynamics of plc gene transcription and α-toxin production during growth of Clostridium perfringens strains with contrasting α-toxin production

    DEFF Research Database (Denmark)

    Abildgaard, Lone; Schramm, Andreas; Rudi, Knut;

    2009-01-01

    The aim of the present study was to investigate transcription dynamics of the α-toxin-encoding plc gene relative to two housekeeping genes (gyrA and rplL) in batch cultures of three Clostridium perfringens strains with low, intermediate, and high levels of α-toxin production, respectively. The plc...... transcript level was always low in the low α-toxin producing strain. For the two other strains, plc transcription showed an inducible pattern and reached a maximum level in the late exponential growth phase. The transcription levels were however inversely correlated to α-toxin production for the two strains....... We propose that this discrepancy is due to differences in plc translation rates between the strains and that strain-specific translational rates therefore must be determined before α-toxin production can be extrapolated from transcript levels in C. perfringens....

  6. Automatic high power RF processing system using PLC

    International Nuclear Information System (INIS)

    We have developed the automatic control system using Programmable Logic Controller (PLC) for the high power RF processing, which is used for the C-band (5712-MHz) accelerating structure and the klystron in SPring-8 Compact SASE Source (SCSS) project. The PLC has been used in industry to have many advantages, such as reliable, compact, low-cost. In addition the PLC is recently able to communicate with the upper-layer controller through a network. We use this system for the klystron RF power test. In this paper, we will describe the configuration of the system and the detail of the high power RF processing. (author)

  7. Practical Curriculum Design Based on Food Motoring System of PLC

    Directory of Open Access Journals (Sweden)

    Jie Yang

    2015-03-01

    Full Text Available The study designed a kind of stepping food motoring control system based on PLC and touch screen, complete the secondary design and renovation based on PLC technology. According to the trend that the PLC new technology is widely and deeply applied into modern food industrial production, combining students electrician, maintenance electrician basic skill training needs to make a reformation of the food motoring system of student training curriculum. This system has simple structure, stable performance, economic value, outstanding using effect, remarkable specific design example and expected effect.

  8. Isolation of cDNA encoding the catalytic site of phosphatidylinositol-specific phospholipase C from Coffea arabica L.: Recombinant expression and peptide purification

    OpenAIRE

    Sánchez-Cach, Lucila A; Ortiz-García, Matilde M; Minero-García, Yereni; Muñoz-Sánchez, J. Armando; Hernández-Sotomayor, SM Teresa; Suárez-Solís, Víctor M; De los Santos-Briones, César

    2008-01-01

    A cDNA encoding the catalytic site of a phosphatidylinositol-specific phospholipase C (PI-PLC) was isolated from Coffea arabica suspension cells. The cDNA (designated CaPLC) encodes a polypeptide of 308 amino acids, containing the catalytic X and Y domains, and has 99% identity to the soybean gene. Recombinant CaPLC protein was expressed in Escherichia coli, purified, and used to produce a polyclonal antibody. The peptide has a molecular mass of 27 kDa on sodium dodecyl sulfate-polyacrylamide...

  9. Study on Measuring System of Casing Machine Based on PLC

    Directory of Open Access Journals (Sweden)

    Huiqiang Wang

    2014-05-01

    Full Text Available According to the technology requirements of measurement for animal casing, in this paper, we use PLC and touch screen as the control core, the electromechanical integration design ideas to research the methods and principles for casing measure, and analyze the mechanical structures and mechanical characteristics of casing machine. As the control core, the programmable logic controller (PLC ensures that the whole control system has high precision, high stability, high reliability during the operation time. Through PLC and touch screen well match with PLC which make the casing machine more convenient to operate the whole system. The configuration software form has a brief and intuitive interface on touch screen which makes it easy to use. The mechanical structure and control system of this casing machine are more stable, more reliable and with high anti-interference ability, and satisfies various requirements for animal casings, easy and convenient to operate.

  10. Programmable logic controller (PLC) for safety systems of nuclear plants

    International Nuclear Information System (INIS)

    Full text: A programmable logic controller (PLC) has been developed by RCnD, BARC for use in the safety critical systems in nuclear power plants. This PLC uses qualified hardware developed in RCnD for use in NPP. The programming software conforms to IEC-61131 part 3. The application programming is done on function block diagram (FBD) editor and the FBD is automatically converted into code in high level language (C / C++). This feature makes the application easily decipherable and therefore easily subjected to reviews and other validation techniques. The key to make quality software for use in nuclear systems is to enforce various standards in the design and development of the software, something, which is not possible to do with a commercially available PLC. This PLC with its software completely transparent lends itself to rigorous verification and validation easily

  11. Applying Model Checking to Industrial-Sized PLC Programs

    CERN Document Server

    AUTHOR|(CDS)2079190; Darvas, Daniel; Blanco Vinuela, Enrique; Tournier, Jean-Charles; Bliudze, Simon; Blech, Jan Olaf; Gonzalez Suarez, Victor M

    2015-01-01

    Programmable logic controllers (PLCs) are embedded computers widely used in industrial control systems. Ensuring that a PLC software complies with its specification is a challenging task. Formal verification has become a recommended practice to ensure the correctness of safety-critical software but is still underused in industry due to the complexity of building and managing formal models of real applications. In this paper, we propose a general methodology to perform automated model checking of complex properties expressed in temporal logics (\\eg CTL, LTL) on PLC programs. This methodology is based on an intermediate model (IM), meant to transform PLC programs written in various standard languages (ST, SFC, etc.) to different modeling languages of verification tools. We present the syntax and semantics of the IM and the transformation rules of the ST and SFC languages to the nuXmv model checker passing through the intermediate model. Finally, two real cases studies of \\CERN PLC programs, written mainly in th...

  12. Study on Measuring System of Casing Machine Based on PLC

    OpenAIRE

    Huiqiang Wang; Yanqiu Xing; Weilian Sun

    2014-01-01

    According to the technology requirements of measurement for animal casing, in this paper, we use PLC and touch screen as the control core, the electromechanical integration design ideas to research the methods and principles for casing measure, and analyze the mechanical structures and mechanical characteristics of casing machine. As the control core, the programmable logic controller (PLC) ensures that the whole control system has high precision, high stability, high reliability during the o...

  13. Technology PLC - Power Line Communication, Used in Monitoring Systems Online

    OpenAIRE

    Vilson Gruber; Roderval Marcelino; Juarez Bento Da Silva

    2013-01-01

    This paper is developing a system for monitoring, supervision and acquisition parameters using Power Line Communication technology. The system consists of a web microserver using the power grid for the transmission and reception of data through technology PLC (Power Line Communication), as a solution for remote monitoring of temperature parameters of a mini turbine bench. In this article were performed comparative tests between the PLC and LAN technology in two distinct environments: resident...

  14. Modulace v PLC systémech

    OpenAIRE

    Procházka, Vít

    2008-01-01

    V bakalářské práci je popsán princip a způsob komunikace po elektrické napájecí síti PLC, možnosti využití, jak v průmyslových aplikacích, ale hlavně v širokopásmových systémech, které mohou představovat slibnou konkurenci k tradičním širokopásmovým přístupům, např. přípojkám xDSL, aniž by bylo nutné instalovat nové vodiče, WIFI. Jsou popsány problémy, které se mohou vyskytnout v komunikačním kanálu z hlediska elektromagnetické kompatibility a různých druhů rušení. Pro přenos datového signálu...

  15. Safety culture development in nuclear electric plc

    International Nuclear Information System (INIS)

    Nuclear Electric plc (NE) has always given the highest priority to safety. However, past emphasis has been directed towards ensuring safety thorough engineering design and hazard control procedures. Whilst the company did achieve high safety standards, particularly with respect to accidents, it was recognized that further improvements could be obtained. Analysis of the safety performance across a wide range of industries showed that the key to improving safety performance lay in developing a strong safety culture within the company. Over the last five years, NE has made great strides to improve its safety culture. This has resulted in a considerable improvement in its measured safety performance indicators, such as the number of incidents at international nuclear event scale (INES) rating 1, the number of lost time accidents and the collective radiation dose. However, despite this success, the company is committed to further improvement and a means by which this process becomes self-sustaining. In this way the company will achieve its prime goal, to ''ensure the safety of people, plant and the environment''. The paper provides an overview of the development of safety culture in NE since its formation in November 1989. It describes the research and international developments that have influenced the company's understanding of safety culture, the key initiatives that the company has undertaken to enhance its safety culture and the future initiatives being considered to ensure continual improvement. (author). 5 refs, 2 figs, 2 tabs

  16. Investigation of PLC band nucleation in AA5754

    International Nuclear Information System (INIS)

    Highlights: ► Simultaneous propagation of bands in transverse and longitudinal directions. ► PLC band nucleation at the back front of Lüders bands. ► Characteristic time of critical strain decreases with strain rate. ► Simultaneous existence of two type-B bands at specimen shoulder. - Abstract: The purpose of the present work is the experimental investigation of the nucleation of PLC deformation bands in the aluminium alloy AA5754. The PLC bands are investigated using both mechanical methods and infrared (IR) thermography. The latter employs a high-speed IR camera which captures local changes of radiated power resulting from mechanical dissipation and heating due to the nucleation of PLC bands. The resulting IR images are used to determine spatio-temporal power field variations via image subtraction. Furthermore, band trajectories obtained from the IR images are used to study possible correlations between the spatio-temporal evolution of stress and radiated power in the specimens and PLC band development.

  17. Efficient Implementation of a Symbol Timing Estimator for Broadband PLC

    Directory of Open Access Journals (Sweden)

    Francisco Nombela

    2015-08-01

    Full Text Available Broadband Power Line Communications (PLC have taken advantage of the research advances in multi-carrier modulations to mitigate frequency selective fading, and their adoption opens up a myriad of applications in the field of sensory and automation systems, multimedia connectivity or smart spaces. Nonetheless, the use of these multi-carrier modulations, such as Wavelet-OFDM, requires a highly accurate symbol timing estimation for reliably recovering of transmitted data. Furthermore, the PLC channel presents some particularities that prevent the direct use of previous synchronization algorithms proposed in wireless communication systems. Therefore more research effort should be involved in the design and implementation of novel and robust synchronization algorithms for PLC, thus enabling real-time synchronization. This paper proposes a symbol timing estimator for broadband PLC based on cross-correlation with multilevel complementary sequences or Zadoff-Chu sequences and its efficient implementation in a FPGA; the obtained results show a 90% of success rate in symbol timing estimation for a certain PLC channel model and a reduced resource consumption for its implementation in a Xilinx Kyntex FPGA.

  18. Hitsausprosessin 3D-simulointi PLC-rajapinnalla

    OpenAIRE

    Ali-Jaakkola, Ilmari

    2016-01-01

    Tämän insinöörityön tilaajana oli Visual Components Oy, joka tuottaa tehdassimulointiohjelmia. Työn tarkoituksena oli simuloida hitsausprosessi, jota ohjataan PLC:llä, ja tehdä tutoriaali PLC:n liittämisestä simulaatioon. Työssä tarkastellaan tehdassimulaatioita sekä niihin usein yhdistettyä etäohjelmointia, virtuaalista käyttöönottoa ja PLC:tä. Lisäksi tutustutaan 3DAutomate tehdassimulaatio-ohjelman käyttämiseen, josta käydään läpi kappaleen luominen ja muokkaaminen sekä simulaation rak...

  19. Opravdanost primjene PLC-a za "manje" aplikacije

    OpenAIRE

    Srpak, Dunja

    2008-01-01

    Primjena programabilnog logičkog upravljača (u daljnjem tekstu PLC, od engl. Programmable Logic Controller) u automatizaciji danas je već znatno raširena, moglo bi se reći "nije ništa novo". Ipak, kad se kaže da je upravljanje nekim strojem, uređajem ili postrojenjem izvedeno PLC-om, većina automatski očekuje kompliciranu i svakako skupu automatiku. Istina je da ozbiljnije, složenije i zahtjevnije izvedbe upravljačkih sklopova danas podrazumijevaju primjenu PLC-a, čak i više njih umreženih u ...

  20. Prototype of Automated PLC Model Checking Using Continuous Integration Tools

    CERN Document Server

    Lettrich, Michael

    2015-01-01

    To deal with the complexity of operating and supervising large scale industrial installations at CERN, often Programmable Logic Controllers (PLCs) are used. A failure in these control systems can cause a disaster in terms of economic loses, environmental damages or human losses. Therefore the requirements to software quality are very high. To provide PLC developers with a way to verify proper functionality against requirements, a Java tool named PLCverif has been developed which encapsulates and thus simplifies the use of third party model checkers. One of our goals in this project is to integrate PLCverif in development process of PLC programs. When the developer changes the program, all the requirements should be verified again, as a change on the code can produce collateral effects and violate one or more requirements. For that reason, PLCverif has been extended to work with Jenkins CI in order to trigger automatically the verication cases when the developer changes the PLC program. This prototype has been...

  1. Development of radioactive source scanner based on PLC

    International Nuclear Information System (INIS)

    The radioactive radial uniformity of 68Ge line radioactive sources is a critical quality parameter. The radioactive source scanner with linear scanning function is developed by making use of high-speed pulse counters, high-speed pulse output ports, and the powerful instruction system of Siemens S7-200 series programmable logic controller (PLC). A computer used as a host computer of the instrument communicate with. the PLC by point to point interface (PPI) protocol, The instrument with functions of data collection, transmission, displaying, saving, motion control and instrument parameter settings, can be used to measure the radioactive radial uniformity and total activity of line radioactive source. The advantages of Using the PLC to develop nuclear instrumentation are development speed, strong anti-interference ability, and low-cost. This paper mainly describes the control system implementation and feature of the instrument. (authors)

  2. Performance Evaluation of CE-OFDM in PLC Channel

    Directory of Open Access Journals (Sweden)

    El Ghzaoui Mohammed

    2011-02-01

    Full Text Available One major drawback associated with an OFDM system is that the transmitter’s output signal may have a high peak-to-average ratio (PAPR. High levels of PAR may be a limiting factor for power line communication (PLC where regulatory bodies have fixed the maximum amount of transmit power. To overcome this problem, many approaches have been presented in the literature. One potential solution for reducing the peak-to-average power ratio (PAPR in an OFDM system is to utilize a constant envelope OFDM (CE-OFDM system. This paper describes a CE-OFDM based modem for Power Line Communications (PLC over the low voltage distribution network. The impact of the electrical appliances on the signal transmission is investigated. The good performances of the BER have been checked by the simulation platform of real PLC channel using Matlab. Finally, CE-OFDM-CPM is compared with conventional OFDM under HomePlug AV.

  3. MIMO communications for inhome PLC networks: measurements and results up to 100 MHz

    OpenAIRE

    HASHMAT, Rehan; Pagani, Pascal; CHONAVEL, Thierry

    2010-01-01

    Power Line Communications (PLC) is used for information exchange over the lines installed for delivering the electrical power. Inhome PLC is a technology which delivers telecom services to every corner of a household through already existing electrical wiring. In recent years, PLC has emerged as a potential candidate for domestic high bit rate services. The current inhome PLC technology, based on Single-Input Single-Output (SISO) configuration, under achieves the capacity offered by the physi...

  4. Global regulator Anr represses PlcH phospholipase activity in Pseudomonas aeruginosa when oxygen is limiting

    OpenAIRE

    Jackson, Angelyca A.; Daniels, Emily F.; Hammond, John H.; Willger, Sven D.; Hogan, Deborah A.

    2014-01-01

    Haemolytic phospholipase C (PlcH) is a potent virulence and colonization factor that is expressed at high levels by Pseudomonas aeruginosa within the mammalian host. The phosphorylcholine liberated from phosphatidylcholine and sphingomyelin by PlcH is further catabolized into molecules that both support growth and further induce plcH expression. We have shown previously that the catabolism of PlcH-released choline leads to increased activity of Anr, a global transcriptional regulator that pro...

  5. Accounting Ratios and Bankruptcy Prediction: Woolworths Geoup PLC as A Case Study

    OpenAIRE

    Okuguni, Anthony I

    2012-01-01

    ABSTRACT This research attempts to ascertain the predictive ability of accounting ratios using Woolworths Group plc. This was achieved by carrying out Financial Ratio Analysis, Altman Z-score, Robertson R-ratio and Trend Analysis of Woolworths Group plc. In addition, the causes of bankruptcy at Woolworths Group plc would be ascertained. Finally the research would use mathematical models to ascertain the predictability of bankruptcy at Woolworths Group plc. The main findings of this re...

  6. Transforming PLC Programs into Formal Models for Verification Purposes

    CERN Document Server

    Darvas, D; Blanco, E

    2013-01-01

    Most of CERN’s industrial installations rely on PLC-based (Programmable Logic Controller) control systems developed using the UNICOS framework. This framework contains common, reusable program modules and their correctness is a high priority. Testing is already applied to find errors, but this method has limitations. In this work an approach is proposed to transform automatically PLC programs into formal models, with the goal of applying formal verification to ensure their correctness. We target model checking which is a precise, mathematical-based method to check formalized requirements automatically against the system.

  7. Model-based automated testing of critical PLC programs.

    CERN Document Server

    Fernández Adiego, B; Tournier, J-C; González Suárez, V M; Bliudze, S

    2014-01-01

    Testing of critical PLC (Programmable Logic Controller) programs remains a challenging task for control system engineers as it can rarely be automated. This paper proposes a model based approach which uses the BIP (Behavior, Interactions and Priorities) framework to perform automated testing of PLC programs developed with the UNICOS (UNified Industrial COntrol System) framework. This paper defines the translation procedure and rules from UNICOS to BIP which can be fully automated in order to hide the complexity of the underlying model from the control engineers. The approach is illustrated and validated through the study of a water treatment process.

  8. 76 FR 29814 - Statutory Debarment and Reinstatement of BAE Systems plc

    Science.gov (United States)

    2011-05-23

    ... Statutory Debarment and Reinstatement of BAE Systems plc Bureau of Political-Military Affairs; Statutory Debarment and Reinstatement of BAE Systems plc and Policy of Denial Concerning Certain Non-U.S. Subsidiaries... BAE Systems plc (``BAES'') as a result of its conviction of conspiracy (18 U.S.C. 371) to...

  9. Integration of the HVCM PLC into the PEFP Control System

    International Nuclear Information System (INIS)

    The High Voltage Converter Modulators (HVCM) for the 100-MeV accelerator was installed to drive the 20-MeV linac. There are two klystrons in the 20-MeV linac and one modulator was used to drive two klystrons simultaneously. The HVCM for the 20-MeV linac area in Korea Atomic Energy Research Institute (KAERI) is shown in Fig. 1. We were faced with the necessity of integrating Programmable Logic Controllers (PLCs) for the HVCM into the Proton Engineering Frontier Project (PEFP) control system. At the PEFP, Experimental Physics and Industrial Control System (EPICS) has become the most widely used solution for building control systems for 100MeV proton accelerator. The EPICS as a standard development tool is a distributed architecture that provides several solutions such as independent programming tools for operating system, operator interface tools, and archiving tools. Although EPICS is used directly in our control system, HVCMs were delivered with the Allen-Bradley ControlLogix as a PLC. The industrial PLC has been verified for safety systems. We need to connect an interface from our EPICS control system to AB-PLC using Ethernet/IP (ControlNet over Ethernet) protocol over Ethernet. In this paper, we will present the communication protocol and EPICS IOC installation for the EPICS based PLC control system

  10. PowerGen plc report and accounts 1994

    International Nuclear Information System (INIS)

    The annual report and accounts of PowerGen plc for the year 1994 are presented. Financial highlights are quoted, followed by the Chairman's statement, reviews by the Chief Executive and Financial Directors, reports by the Auditors and Directors, balance sheets and details of the consolidated profit and loss account and principal accounting policies. A four year summary and shareholder information are included. (UK)

  11. Multiprog Virtual Laboratory Applied to PLC Programming Learning

    Science.gov (United States)

    Shyr, Wen-Jye

    2010-01-01

    This study develops a Multiprog virtual laboratory for a mechatronics education designed to teach how to programme a programmable logic controller (PLC). The study was carried out with 34 students in the Department of Industry Education and Technology at National Changhua University of Education in Taiwan. In total, 17 students were assigned to…

  12. PI-PLC: Phosphoinositide-Phospholipase C in Plant Signaling

    NARCIS (Netherlands)

    T. Munnik

    2014-01-01

    Historically, phosphoinositide-specific phospholipase C (PI-PLC) catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to generate the second messengers, inositol 1,4,5-trisphosphate (InsP3) and diacylglycerol (DAG), which release Ca2+ from intracellular stores and activate member

  13. Activation of the latent PlcR regulon in Bacillus anthracis

    OpenAIRE

    Sastalla, Inka; Maltese, Lauren M.; Pomerantseva, Olga M.; Pomerantsev, Andrei P; Keane-Myers, Andrea; Stephen H Leppla

    2010-01-01

    Many genes in Bacillus cereus and Bacillus thuringiensis are under the control of the transcriptional regulator PlcR and its regulatory peptide, PapR. In Bacillus anthracis, the causative agent of anthrax, PlcR is inactivated by truncation, and consequently genes having PlcR binding sites are expressed at very low levels when compared with B. cereus. We found that activation of the PlcR regulon in B. anthracis by expression of a PlcR–PapR fusion protein does not alter sporulation in strains c...

  14. Activated PLC-γ1 is Catalytically Induced at LAT but Activated PLC-γ1 is Localized at both LAT- and TCR-Containing Complexes

    OpenAIRE

    Cruz-Orcutt, Noemi; Vacaflores, Aldo; Connolly, Sean F.; Bunnell, Stephen C.; Houtman, Jon C.D.

    2014-01-01

    Phospholipase C-γ1 (PLC-γ1) is a key regulator of T cell receptor (TCR)-induced signaling. Activation of the TCR enhances PLC-γ1 enzymatic function, resulting in calcium influx and the activation of PKC family members and RasGRP. The current model is that phosphorylation of LAT tyrosine 132 facilitates the recruitment of PLC-γ1, leading to its activation and function at the LAT complex. In this study, we examined the phosphorylation kinetics of LAT and PLC-γ1 and the cellular localization of ...

  15. 78 FR 23316 - The Royal Bank of Scotland plc, et al.; Notice of Application and Temporary Order

    Science.gov (United States)

    2013-04-18

    ... COMMISSION The Royal Bank of Scotland plc, et al.; Notice of Application and Temporary Order April 12, 2013.... APPLICANTS: The Royal Bank of Scotland plc (``RBS plc''), Citizens Investment Advisors (``Citizens IA''), a... Exchange Commission, 100 F Street NE., Washington, DC 20549-1090. Applicants: RBS plc, RBS, Gogarburn,...

  16. Úzkopásmová PLC komunikace se standardy G3-PLC, PRIME a IEEE-1901.2

    OpenAIRE

    Skrášek, Tomáš

    2015-01-01

    Diplomová práce pojednává o standardech úzkopásmové PLC komunikace. V teoretické části jsou popsány všechny dostupné OFDM standardy, mezi něž patří G3-PLC, PRIME, IEEE-1901.2 a G.hnem. Praktická část se zabývá standardy PRIME a G3-PLC. Dále je také porovnán systém OFDM se systémem komunikace na jedné nosné frekvenci v prostředí s reálným rušením. V poslední části je popsán návrh dvou firmware pro PLC modemy Texas Instruments TMDSPLCKIT-V3, které umožňují UART komunikaci a dálkový sběr dat. K ...

  17. A single substitution in 5'-untranslated region of plcB is involved in enhanced broad-range phospholipase C activity in Listeria monocytogenes strain H4

    Institute of Scientific and Technical Information of China (English)

    Fan Bai; Jianshun Chen; Qiaomiao Chen; Xiaokai Luo; Weihuan Fang; Lingli Jiang

    2011-01-01

    To examine whether the in vitro phospholipase activity in Listeria monocytogenes strain H4 was due to two nucleotide mutations (C to T at position -26 and A to G at position + 1) in plcB or resulted from regulatory activation, two mutants H4-plcB-ml (single mutation at position -26) and H4-plcB-m2 (substitution at both positions) were constructed by site-directed mutagenesis. It was found that the two mutants had significantly lower transcription of plcB than their parent strain H4 and did not show phospho-Hpase activity on the egg yolk agar, implying that the apparent phospholipase activity of strain H4 could be related to single substitution at position -26 ofplcB, most probably by its 5t-untranslated region (5'-UTR) regulation mechanism. Tn917-based transposon mutagenesis generated eight L. monocytogenes mutants lacking phospholipase activity among 560 mutant candidates. Seven mutants had transposon insertion into prfA (encoding positive regulatory factor A) open reading frame, whereas only one mutant (WF-L127) was inserted into the P1 promoter region ofprfA (prfAP1). Transcription of major virulence genes was significantly lower in both types of mutants than in their parent strain H4. Disruption of prfAP1 in WFL127 abolished its phospholipase C activity but did not change its hemolytic phenotype, indicating that plcB was more dependent on prfA regulation than hly. Taken together, this study presents some evidence for the regulation ofplcB expression by its 5'-UTR mechanism.

  18. The phenotype of a phospholipase C (plc-1) mutant in a filamentous fungus, Neurospora crassa.

    Science.gov (United States)

    Lew, Roger R; Giblon, Rachel E; Lorenti, Miranda S H

    2015-09-01

    In the filamentous fungus Neurospora crassa, phospholipase C may play a role in hyphal extension at the growing tips as part of a growth-sensing mechanism that activates calcium release from internal stores to mediate continued expansion of the hyphal tip. One candidate for a tip-localized phospholipase C is PLC-1. We characterized morphology and growth characteristics of a knockout mutant (KO plc-1) and a RIP mutated strain (RIP plc-1) (missense mutations and a nonsense mutation render the gene product non-functional). Growth and hyphal cytology of wildtype and KO plc-1 were similar, but the RIP plc-1 mutant grew slower and exhibited abnormal membrane structures at the hyphal tip, imaged using the fluorescence dye FM4-64. To test for causes of the slower growth of the RIP plc-1 mutant, we examined its physiological poise compared to wildtype and the KO plc-1 mutant. The electrical properties of all three strains and the electrogenic contribution of the plasma membrane H(+)-ATPase (identified by cyanide inhibition) were the same. Responses to high osmolarity were also similar. However, the RIP plc-1 mutant had a significantly lower turgor, a possible cause of its slower growth. While growth of all three strains was inhibited by the phospholipase C inhibitor 3-nitrocoumarin, the RIP plc-1 mutant did not exhibit hyphal bursting after addition of the inhibitor, observed in both wildtype and the KO plc-1 mutant. Although the plc-1 gene is not obligatory for tip growth, the phenotype of the RIP plc-1 mutant - abnormal tip cytology, lower turgor and resistance to inhibitor-induced hyphal bursting - suggest it does play a role in tip growth. The expression of a dysfunctional plc-1 gene may cause a shift to alternative mechanism(s) of growth sensing in hyphal extension. PMID:26212074

  19. Activation of the latent PlcR regulon in Bacillus anthracis.

    Science.gov (United States)

    Sastalla, Inka; Maltese, Lauren M; Pomerantseva, Olga M; Pomerantsev, Andrei P; Keane-Myers, Andrea; Leppla, Stephen H

    2010-10-01

    Many genes in Bacillus cereus and Bacillus thuringiensis are under the control of the transcriptional regulator PlcR and its regulatory peptide, PapR. In Bacillus anthracis, the causative agent of anthrax, PlcR is inactivated by truncation, and consequently genes having PlcR binding sites are expressed at very low levels when compared with B. cereus. We found that activation of the PlcR regulon in B. anthracis by expression of a PlcR-PapR fusion protein does not alter sporulation in strains containing the virulence plasmid pXO1 and thereby the global regulator AtxA. Using comparative 2D gel electrophoresis, we showed that activation of the PlcR regulon in B. anthracis leads to upregulation of many proteins found in the secretome of B. cereus, including phospholipases and proteases, such as the putative protease BA1995. Transcriptional analysis demonstrated expression of BA1995 to be dependent on PlcR-PapR, even though the putative PlcR recognition site of the BA1995 gene does not exactly match the PlcR consensus sequence, explaining why this protein had escaped recognition as belonging to the PlcR regulon. Additionally, while transcription of major PlcR-dependent haemolysins, sphingomyelinase and anthrolysin O is enhanced in response to PlcR activation in B. anthracis, only anthrolysin O contributes significantly to lysis of human erythrocytes. In contrast, the toxicity of bacterial culture supernatants from a PlcR-positive strain towards murine macrophages occurred independently of anthrolysin O expression in vitro and in vivo. PMID:20688829

  20. British Nuclear Fuels plc: report and accounts 1987-88

    International Nuclear Information System (INIS)

    The Energy Committee has considered the report and accounts of BNFL (British Nuclear Fuels PLC) for the year 1987-88. The report looks at BNFL as a government owned PLC - its activities and financial performance. Various questions are raised about the underlying financial position justifying the optimism portrayed in the report and accounts. The impact of cost-plus contracts on UK customers is examined. The economics of THORP (Thermal Oxide Reprocessing Plant) are also examined especially as the escalation in the cost of constructing THORP means that a substantial loss will be made in the reprocessing of waste for which contracts were signed in the late 1960s or early 1970s. The main conclusions of the report are summarized. One of these is that the UK must be cautious about becoming a repository of foreign nuclear waste. Other specific recommendations are made - some about the decommissioning of BNFL plant. (UK)

  1. Design of a Virtual PLC Using Lab View

    Directory of Open Access Journals (Sweden)

    Muntaser Momani

    2010-05-01

    Full Text Available Using LabVIEW environment a trial has been made to create a set of programmable virtual instruments, which resemble the traditional PLC programmable functions and networks. The target of this work is to improve the programmability of PC-based control systems. Bringing the PLC to the industrial type computer it becomes possible to make use of the advantages of computer based DCSs. It will be possible to have unlimited number of programmable objects, and to run more than one program at the same time. By utilizing LabVIEW front panel it is an easy job to realize MMI as required, and by creating sub VIs the program becomes more compact and easier to debug.

  2. Bringing Automated Formal Verification to PLC Program Development

    CERN Document Server

    Fernández Adiego, Borja; Blanco Viñuela, Enrique

    Automation is the field of engineering that deals with the development of control systems for operating systems such as industrial processes, railways, machinery or aircraft without human intervention. In most of the cases, a failure in these control systems can cause a disaster in terms of economic losses, environmental damages or human losses. For that reason, providing safe, reliable and robust control systems is a first priority goal for control engineers. Ideally, control engineers should be able to guarantee that both software and hardware fulfill the design requirements. This is an enormous challenge in which industry and academia have been working and making progresses in the last decades. This thesis focuses on one particular type of control systems that operates industrial processes, the PLC (Programmable Logic Controller) - based control systems. Moreover it targets one of the main challenges for these systems, guaranteeing that PLC programs are compliant with their specifications. Traditionally ...

  3. A Software Agent for Automatic Creation of a PLC Program

    Directory of Open Access Journals (Sweden)

    Walid M. Aly

    2008-01-01

    Full Text Available Using structured design techniques to design a Programmable Logical Control (PLC program would decrease the time needed for debugging and produces a concise bug free code. This study is concerned with the design of a software agent for automatic creation of code for a PLC program that can be downloaded on a Siemens Step 7 series. The code is generated according to the syntax rules for the AWL Language, AWL is the abbreviation for the germen word Anweisungsliste which means Instruction List The proposed system uses object oriented approach to transfer the design specification into an object that adequately describes the system using the state based design technique. The industrial system specifications are supplied by the user through a simple Graphical User Interface (GUI environment These specification define the attributes vales of an object oriented class describing the control system, all the functions needed to generate the code are encapsulated in the class.

  4. Performance Evaluation of CE-OFDM in PLC Channel

    Directory of Open Access Journals (Sweden)

    El ghzaoui Mohammed, Belkadid Jamal, Benbassou Ali

    2011-02-01

    Full Text Available One major drawback associated with an OFDM system is that the transmitter’soutput signal may have a high peak-to-average ratio (PAPR. High levels of PARmay be a limiting factor for power line communication (PLC where regulatorybodies have fixed the maximum amount of transmit power. To overcome thisproblem, many approaches have been presented in the literature. One potentialsolution for reducing the peak-to-average power ratio (PAPR in an OFDMsystem is to utilize a constant envelope OFDM (CE-OFDM system. This paperdescribes a CE-OFDM based modem for Power Line Communications (PLCover the low voltage distribution network. The impact of the electrical applianceson the signal transmission is investigated. The good performances of the BERhave been checked by the simulation platform of real PLC channel using Matlab.Finally, CE-OFDM-CPM is compared with conventional OFDM under HomePlugAV..

  5. Fifth Baltic Sea pollution load compilation (PLC-5)

    Energy Technology Data Exchange (ETDEWEB)

    Knuuttila, S.; Svendsen, L.M.; Staaf, H.; Kotilainen, P.; Boutrup, S.; Pyhala, M.; Durkin, M.

    2011-07-01

    This report includes the main results from the Fifth Pollution Load Compilation abbreviated PLC-5. It includes quantified annual waterborne total loads (from rivers, unmonitored and coastal areas as well as direct point and diffuse sources discharging directly to the Baltic Sea) from 1994 to 2008 to provide a basis for evaluating any decreasing (or increasing) trends in the total waterborne inputs to the Baltic Sea. Chapter 1 contains the objectives of PLC and the framework on classification of inputs and sources. Chapter 2 includes a short description of the Baltic Sea catchment area, while the methods for quantification and analysis together with quality assurance topics are briefly introduced in Chapter 3. More detailed information on methodologies is presented in the PLC-5 guidelines (HELCOM 2006). Chapter 4 reports the total inputs to the Baltic Sea of nutrients and selected heavy metals. Furthermore, the results of the quatification of discharges and losses of nitrogen and phosphorus from point and diffuse sources into inland surface waters within the Baltic Sea catchment area (source-oriented approach or gross loads) as well as the total load to the maritime area (load-oriented approarch or net loads) in 2006 are shown. Typically, results are presented by country and by main Baltic Sea sub-region. In Chapter 5, flow normalization is introduced and the results of trend analyses on 1994-2008 time series data on total waterborne loads of nitrogen and phosphorus are given together with a first evaluation of progress in obtaining the provisional reduction targets by country and by main Baltic Sea sub-region. Chapter 6 includes discussion of some of the main conclusions and advice for future PLCs. The annexes contain the flow-normalized annual load data and figures and tables with results from the PLC-5.

  6. Online Monitoring of PLC Based Pressure Control System

    OpenAIRE

    Helen Maria Sabu; Aravind V B; Apurva Sullerey; Binson V A

    2015-01-01

    This paper deals with the online monitoring of pressure control system using OPC servers. Here, the pressure from a pressure control system is transmitted to a Siemens PLC which is being monitored and controlled remotely by using OPC servers thereby offering a provision of debugging and changing process parameters easily. OLE for Process Control (OPC) is the industry standard that incorporates real time software technology for industrial automation and process control. Online monitoring of th...

  7. Articulating Factors Defining RMS Delay Spread in LV PLC Networks

    OpenAIRE

    Sabih Güzelgöz; Hasan Basri Çelebi; Hüseyin Arslan

    2010-01-01

    Communication over the power line network (PLN) referred as power line communication (PLC) has a long history of narrowband applications. With the recent developments in the field of digital communications, current interest is to exploit this medium for wideband communications for several applications such as Internet access, home networking, and in-vehicle data communication. In line with this recently emerging interest which envisions the conversion of a power transmission netwo...

  8. Improving work efficiency in Traffic Planning : Finnair Plc

    OpenAIRE

    Tonteri, Antti

    2015-01-01

    The purpose of the present thesis was to find out the most important improvement areas in the work of Traffic Planning team, which is part of Resource Management department at Finnair Plc. Finnair is a Finnish commercial airline focusing on the traffic between Europe and Asia. The team members have reported dissatisfaction about the working environment especially during the last two years. The objective of this this study was to identify the main problem areas and find solution(s) to improve ...

  9. PLC based control system and maintenance activities at NCAR, Bilaspur

    International Nuclear Information System (INIS)

    A 3.0 MV high current low energy Pelletron Accelerator facility (Model 9SDH-4, NEC, USA) with TORUIS (ion source for H+ and He2+ beam current H+ ion ∼ 50μA @ 6 MeV, He2+ at ∼ 10μA) and SNICS-II ion source for heavy ions has been commissioned as 'National Centre for Accelerator Based Research' in the Department of Pure and Applied Physics, Guru Ghasidas Vishwavidyalaya. In this paper, we detail out the control system developed and implemented at NCAR. The basic idea of controlling the machine is by providing the output signal through PLC to ACPC of accelerator using user interface points provided by the manufacturer. The PLC based system generates output signal once it receives the feedback signals from search and secure switches, door lock switches and scram switches interlocked with PLC. The output is controlled by ladder logic and is activated only when all the radiation monitors are in healthy state and outside radiations monitor having low radiation level. The details of control system and maintenance activities will be discussed in the paper

  10. 78 FR 54149 - Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines

    Science.gov (United States)

    2013-09-03

    ..., Amendment 39-17061 (77 FR 31176, May 25, 2012), and adding the following new AD: 2013-15-10 Rolls-Royce plc...-17061 (77 FR 31176, May 25, 2012). (c) Applicability This AD applies to Rolls-Royce plc (RR) RB211-Trent... approved for IBR on June 29, 2012, (77 FR 31176, May 25, 2012). (i) Rolls-Royce plc RB211 Trent 700...

  11. A study of PLC system vulnerability checklists in nuclear power plants

    International Nuclear Information System (INIS)

    Because the design of the PLCs (Programmable Logic Controller) in the I and C (Instrument and Control) systems for NPP (Nuclear Power Plant) were carried out independently, the problems of cyber security were not addressed in the PLC system designs. Recently, the analysis and the countermeasure development for the PLC systems became mandatory due to the developments in cyber attack techniques and the increasingly revealed vulnerability to such attacks. A comparative analysis on the cyber security checklist of PLC in industry control system and in NPP systems was carried out, and in this paper, the cyber security regulatory trend and the PLC usage status are described

  12. Process operation and control by PLC system for Kroll's reduction of ZrCl4

    International Nuclear Information System (INIS)

    For automatic monitoring and control of pressure and temperature of Kroll's reduction of zirconium chloride, the PLC system has been designed, manufactured, installed and commissioned for one reduction unit at ZSP. The unit has been successfully in use on line with PLC for about one hundred batches. The safety features have been ascertained for regular use of PLC with provision of manual overide, in case of any malfunctioning. The paper is the review of performance and functioning of the system to include the additional features of the PLC for the remaining four such units. (author)

  13. AUTOMATION OF PLC PROGRAMMING WHEN IMPLEMENTING ALGORITHMS OF GUARANTEEING CONTROL

    Directory of Open Access Journals (Sweden)

    M. V. Levinskyi

    2015-05-01

    Full Text Available During developing programs for programmable logic controllers (PLCs the concept of model-oriented design is increasingly used. In particular, usage of Simulink PLC Coder is giving the opportunity to get SCL program codefrom Simulink model which contains certain dynamic elements. Then, for example, this SCL code can be transformed to functional blocks of the Simatic S7-300 (VIPA 300 PLC. This significantly reduces the timerequired to develop code in the language of SCL and reduces requirements for specialists’ qualification when developing control systems. In this article we provide an example of PLC programming automation whenimplementing algorithms of guaranteeing control (AGC. For certain types of technological processes it is typical to contain monotonically increasing function of the effectiveness with fixed one-way restriction in regulations. Forexample, in the grinders, presses, extruders the load current of the drive is stabilized using the change of feed. Energy efficiency of these plants will increase with increasing of the set point (SP to the controller of the drive loadcurrent stabilization loop. However, an increase in SP increases the probability of triggering appropriate protection, for example, as a result of random changes in the properties of raw materials. Therefore, to avoid this accident, thepower of driving motors is often unreasonably overrated. And in this case they are used with currents equal to the half of rated.Systems of guaranteeing control (SGC are used to solve the contradiction between the need to improvethe efficiency and increasing probability of an accident.

  14. Astable multivibrator circuits made with low capacity PLC

    Science.gov (United States)

    Popa, I.; Popa, G. N.; Diniş, C. M.; Deaconu, S. I.

    2016-02-01

    The paper presents three versions of astable multivibrator structures that can be made with PS-3 (Klöckner-Moeller) PLCs. They have, in their composition, two time-on delay timers (TR type) that can be found in PS-3 PLC. For each astable multivibrator circuits present the principle accompanied by graphic representations of the main signals of the astable multivibrator circuits and the mode of operation of these circuits using time diagrams. These astable multivibrator strucures can be used, special, for teaching PLCs.

  15. Automated Verification Environment for TwinCAT PLC Programs

    OpenAIRE

    Beckmann, Andreas

    2013-01-01

    The European XFEL will have three undulator systems SASE1, SASE2, and SASE3 to produce extremely brilliant, ultra-short pulses of x-rays with wavelengths down to 0.1 nm. The undulator gap is adjustable in order to vary photon beam energy. The corresponding motion control is implemented with industrial PCs running Beckhoff TwinCAT Programmable Logic Controllers (PLCs). So far, the functionality of the PLC programs has been verified on system level with the final hardware. This is a time-consum...

  16. Reseach of Control System of Packaging Machine on PLC

    Directory of Open Access Journals (Sweden)

    Xiao SUN

    2013-06-01

    Full Text Available An automatic control system of sampling package for sample coal is designed according to the realities of sample coal powdery materials which are based on controller (PLC. The system can realize the functions of random sampling, auto-weighting, auto-packaging, and auto-sealing off. It also can adjust waste less, control convenient, zero point self-tuning and dynamics weighing measurement. It can be used in sampling weighing package of sample coal. Various performance indicators can be stably and reliably used in field.

  17. Drip irrigation using a PLC based adaptive irrigation system

    OpenAIRE

    Shahidian, Shakib; Serralheiro, Ricardo; Teixeira, José Luís; Santos, Francisco; Oliveira, Mario do Rosário; Costa, José; Toureiro, Célia; Haie, Naim; Machado, Rui

    2009-01-01

    Irrigation is presently the main user of world´s fresh water. Most of it goes to irrigating small plots where it is not feasible to implement full-scale Evapotranspiration based irrigation controllers. The dramatic development of Programmable Logic Controllers, PLCs, and their rather affordable price has made it possible to use them as stand-alone irrigation controllers. In this paper a PLC is used to adapt the daily irrigation amount to actual ETc, using a Hargreaves-Samani type equation. On...

  18. AUTOMATION OF GLASS TEMPERING FURNACE BY USING PLC

    Directory of Open Access Journals (Sweden)

    Abdullah BÜYÜKYILDIZ

    2007-02-01

    Full Text Available In this study, a furnace which is used for observation of environments under high temperature, and also used for manufacturing of glasses which are resisted to high temperature has been designed and implemented. Automation of this system has been done by using PLC. Operating parameters of furnace such as materials entering, the furnace, the local temperature control of furnace, cooling control and materials outing have been sensed with Hall Effect Sensor. Furthermore, the observation of parameters of furnace on screen has been provided with SCADA software. Obtained products have been shown the system works successfully.

  19. PLC Control System of Microwave Drying Apparatus on Honeysuckle

    Directory of Open Access Journals (Sweden)

    Ge Yu

    2015-08-01

    Full Text Available Control system of microwave drying apparatus on Honeysuckle is designed in order to control microwave drying apparatus automatically according to microwave drying process on Honeysuckle and the designed 20 kW circular microwave drying apparatus. PLC which type is CP1E-X20DR-A of Omron Corporation is adopted as controller and program flow chart is designed, the control of temperature, humidity and drying speed is realized according to Honeysuckle drying process requirements. Practice has proved that: the control system designed can control Honeysuckle drying process completely.

  20. File list: DNS.Plc.50.DNase-Seq.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available DNS.Plc.50.DNase-Seq.AllCell hg19 DNase-seq DNase-Seq Placenta SRX089258,SRX121285,...SRX089254,SRX201830,SRX069084,SRX193582,SRX201819,SRX101015,SRX069127,SRX214046 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/DNS.Plc.50.DNase-Seq.AllCell.bed ...

  1. 76 FR 25353 - Hikma Pharmaceuticals PLC; Analysis of Agreement Containing Consent Orders To Aid Public Comment

    Science.gov (United States)

    2011-05-04

    ... Hikma Pharmaceuticals PLC; Analysis of Agreement Containing Consent Orders To Aid Public Comment AGENCY... Pharmaceuticals PLC (``Hikma'') that is designed to remedy the anticompetitive effects of Hikma's acquisition of... Consent Agreement, Hikma would be required to divest to X-Gen Pharmaceuticals, Inc. (``X-Gen'') all...

  2. File list: InP.Plc.20.Input_control.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Plc.20.Input_control.AllCell mm9 Input control Input control Placenta SRX112971...02,SRX404310,SRX1072160,SRX871507,SRX192097,SRX192098,SRX192099,SRX204148 http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Plc.20.Input_control.AllCell.bed ...

  3. File list: InP.Plc.50.Input_control.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  4. File list: InP.Plc.10.Input_control.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  5. File list: InP.Plc.20.Input_control.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Plc.20.Input_control.AllCell hg19 Input control Input control Placenta SRX08036...6,SRX190049 http://dbarchive.biosciencedbc.jp/kyushu-u/hg19/assembled/InP.Plc.20.Input_control.AllCell.bed ...

  6. File list: InP.Plc.05.Input_control.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  7. 77 FR 26216 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-05-03

    ...-23, Amendment 39-17004 (77 FR 20508, April 5, 2012), and adding the following new AD: Rolls-Royce plc..., Amendment 39-17004 (77 FR 20508, April 5, 2012). (c) Applicability This AD applies to Rolls-Royce plc (RR...-06-23, Amendment 39-17004 (77 FR 20508, April 5, 2012), for all RR RB211-Trent 875-17,...

  8. 78 FR 20509 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2013-04-05

    ... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc... (NPRM). SUMMARY: We propose to adopt a new airworthiness directive (AD) for all Rolls-Royce plc...

  9. 78 FR 60658 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2013-10-02

    ..., Amendment 39-16969 (77 FR 13483, March 7, 2012) and adding the following new AD: 2013-19-21 Rolls Royce plc...-16969 (77 FR 13483, March 7, 2012). (c) Applicability This AD applies to the following Rolls-Royce plc... AD 2012-04-13, Amendment 39-16969 (77 FR 13483, March 7, 2012), (``AD 2012-04-13''). AD...

  10. 78 FR 68360 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2013-11-14

    ...: Airworthiness Directive 2013-19-17, Amendment 39-17599 (78 FR 61171, October 3, 2013), currently requires...-01-AD; Amendment 39-17599; AD 2013-19-17] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc.... That AD applies to all Rolls-Royce plc (RR) RB211-535E4-B-37 series turbofan engines. The AD number...

  11. File list: Pol.Plc.20.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  12. File list: Pol.Plc.50.RNA_polymerase_II.AllCell [Chip-atlas[Archive

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  13. File list: Pol.Plc.10.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

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  14. File list: Unc.Plc.10.Unclassified.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  15. File list: Unc.Plc.20.Unclassified.AllCell [Chip-atlas[Archive

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  16. File list: Pol.Plc.05.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

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  17. File list: ALL.Plc.05.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

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  18. File list: Oth.Plc.10.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

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  1. File list: His.Plc.20.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

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    Lifescience Database Archive (English)

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  3. File list: Pol.Plc.10.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

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  5. File list: ALL.Plc.20.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

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  6. File list: DNS.Plc.20.AllAg.Trophoblast_stem_cells [Chip-atlas[Archive

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  7. File list: Pol.Plc.05.RNA_Polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  8. File list: Pol.Plc.50.RNA_polymerase_III.AllCell [Chip-atlas[Archive

    Lifescience Database Archive (English)

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  9. Narrowband Power Line Comunication Based on G3 PLC and PRIME Standarts

    OpenAIRE

    Csiba, P.

    2015-01-01

    This project is focused on narrowband PLC communication. In this project is described the experimental measurements with TMDSPLCKIT-V4 kit from the Texas Instruments company. The measurements are performed in home environment. This kit allows to compare standards PRIME and G3-PLC.

  10. Fc gamma receptor activation induces the tyrosine phosphorylation of both phospholipase C (PLC)-gamma 1 and PLC-gamma 2 in natural killer cells

    OpenAIRE

    1992-01-01

    Crosslinking of the low affinity immunoglobulin G (IgG) Fc receptor (Fc gamma R type III) on natural killer (NK) cells initiates antibody- dependent cellular cytotoxicity. During this process, Fc gamma R stimulation results in the rapid activation of phospholipase C (PLC), which hydrolyzes membrane phosphoinositides, generating inositol-1,4,5- trisphosphate and sn-1,2-diacylglycerol as second messengers. We have recently reported that PLC activation after Fc gamma R stimulation can be inhibit...

  11. The Causes of Labour Turnover in Bottling Companies in Nigeria: A Comparative Study of Coca-Cola Plc and Seven Up Plc. Lagos, Nigeria

    OpenAIRE

    B.A. Chukwu; O.S. Ofotokun; J.T. Fadeji; V.U. Sule

    2013-01-01

    This study compared the causes of labour turnover in Coca-Cola Plc and Seven Up Plc. in Lagos, Nigeria. They are the major producers of soft drink in Nigeria. Labour turnover costs soft drink industries in Nigeria considerable amount of money yearly in recruiting and training replacements. It is an economic drain to the industry. A sizeable income is also incurred through new employees that are more prone to accidents, causes more breakages and make more mistakes than experience workers. Othe...

  12. The Integration of DCS I/O to an Existing PLC

    Science.gov (United States)

    Sadhukhan, Debashis; Mihevic, John

    2013-01-01

    At the NASA Glenn Research Center (GRC), Existing Programmable Logic Controller (PLC) I/O was replaced with Distributed Control System (DCS) I/O, while keeping the existing PLC sequence Logic. The reason for integration of the PLC logic and DCS I/O, along with the evaluation of the resulting system is the subject of this paper. The pros and cons of the old system and new upgrade are described, including operator workstation screen update times. Detail of the physical layout and the communication between the PLC, the DCS I/O and the operator workstations are illustrated. The complex characteristics of a central process control system and the plan to remove the PLC processors in future upgrades is also discussed.

  13. Run-time system based on LinSERCANS and Soft-PLC

    Institute of Scientific and Technical Information of China (English)

    Cunfeng KANG; Chong WANG; Chunmin MA; Xudong HUANG; Renyuan FEI

    2009-01-01

    Soft-PLC with open architecture is the direction of development in industrial automation. This paper discusses the method of communication between the interface functions of LinSERCANS under RTLinux and the external library of Soft-PLC under Windows. Based on the API HOOK theory, the communication between the interface functions of LinSERCANS and the external libraries of Soft-PLC is set up and it solves the calling functions of dynamic link libraries in different operation systems. It is able to combine LinSERCANS with the Soft-PLC, and a run-time system is developed based on the interface technology of the serial real-time communication system (SERCOS) and technology of soft-PLC. This run-time system has been used in all electronic injection molding machines and works very well.

  14. Commercial information system of Slovak Electricity Transmission System, Plc

    International Nuclear Information System (INIS)

    Commercial Information System (CIS), which main role of is to support the commercial activities of the company, is built up by business department of SEPS, Plc in close co-operation with the supplier company Sfera, Plc. The system entries are data from electricity meters obtained by automatized data acquisition and from bugging sheets, contractual hour diagrams, parameters of transmission system like measuring schemes and formulas for calculation of demand and supply, contractual data resulting from agreements, business conditions, URSO decisions and valid legislative. A part of the system is also support of process of data evaluation like calculation of transmitted energy quantity, calculation of actual output diagrams, comparison of actual and contractual diagrams, calculation of system divergence, calculation of demand divergence, etc. The final price for the drawings of services, which are joined with transmission of the electric power will be determined by the accounting process. In this causality there are automatically made out invoices for access, losses, system charges, system services, short-term and long-term transits, divergences and for cross-border transmission auctions. System also generates various specialized synopses and statistics for the evaluation requirements of important aspects and for parameter observations. Successive liberalisation of the electricity power market, changes in legislative and URSO decisions require CIS to be sufficiently configurable and to adapt flexible on the alternating environs. (author)

  15. The Causes of Labour Turnover in Bottling Companies in Nigeria: A Comparative Study of Coca-Cola Plc and Seven Up Plc. Lagos, Nigeria

    Directory of Open Access Journals (Sweden)

    B.A. Chukwu

    2013-09-01

    Full Text Available This study compared the causes of labour turnover in Coca-Cola Plc and Seven Up Plc. in Lagos, Nigeria. They are the major producers of soft drink in Nigeria. Labour turnover costs soft drink industries in Nigeria considerable amount of money yearly in recruiting and training replacements. It is an economic drain to the industry. A sizeable income is also incurred through new employees that are more prone to accidents, causes more breakages and make more mistakes than experience workers. Other loses are also incurred through reduced production, work disruption and increase scrap and overtime as a result of departed workers. A cross-sectional survey was utilized to collect data for answering research questionnaires and testing hypothesis in this study. The data collected from questionnaire instrument were also analysed using percentages (% and Z-test for comparing two proportions. Comparative analysis of the data showed that Seven Up Plc. rated unwillingness to perform as major cause of discharge while Coca-Cola Plc. rated attitudinal causes. Both companies rated unsatisfactory pay as the major cause of resignation. Seven Up Plc. was not significantly better than Coca-Cola Plc. on a hypothesis testing about the difference between proportions of samples. The Null hypothesis assumed that there were no difference in parameters and that the difference observed between sample percent was due to chance.

  16. 78 FR 10172 - Lisa Anne Cornell and G. Ware Cornell, Jr. v. Princess Cruise Lines, Ltd. (Corp), Carnival PLC...

    Science.gov (United States)

    2013-02-13

    ... Lisa Anne Cornell and G. Ware Cornell, Jr. v. Princess Cruise Lines, Ltd. (Corp), Carnival PLC, and..., Jr., hereinafter ``Complainants,'' against Princess Cruise Lines, Ltd (Corp), Carnival plc, and... common carrier for hire of passengers from ports in the United States;'' Respondent Carnival plc ``is...

  17. Participation of PLA2 and PLC in DhL-induced activation of Rhinella arenarum oocytes.

    Science.gov (United States)

    Zapata-Martínez, J; Medina, M F; Gramajo-Bühler, M C; Sánchez-Toranzo, G

    2016-08-01

    Rhinella arenarum oocytes can be artificially activated, a process known as parthenogenesis, by a sesquiterpenic lactone of the guaianolide group, dehydroleucodine (DhL). Transient increases in the concentration of cytosolic Ca2+ are essential to trigger egg activation events. In this sense, the 1-4-5 inositol triphosphate receptors (IP3R) seem to be involved in the Ca2+ transient release induced by DhL in this species. We analyzed the involvement of phosphoinositide metabolism, especially the participation of phospholipase A2 (PLA2) and phospholipase C (PLC) in DhL-induced activation. Different doses of quinacrine, aristolochic acid (ATA) (PLA2 inhibitors) or neomycin, an antibiotic that binds to PIP2, thus preventing its hydrolysis, were used in mature Rhinella arenarum oocytes. In order to assay the participation of PI-PLC and PC- PLC we used U73122, a competitive inhibitor of PI-PLC dependent events and D609, an inhibitor of PC-PLC. We found that PLA2 inhibits quinacrine more effectively than ATA. This difference could be explained by the fact that quinacrine is not a specific inhibitor for PLA2 while ATA is specific for this enzyme. With respect to the participation of PLC, a higher decrease in oocyte activation was detected when cells were exposed to neomycin. Inhibition of PC-PLC with D609 and IP-PLC with U73122 indicated that the last PLC has a significant participation in the effect of DhL-induced activation. Results would indicate that DhL induces activation of in vitro matured oocytes of Rhinella arenarum by activation of IP-PLC, which in turn may induce IP3 formation which produces Ca2+ release. PMID:26350822

  18. A Spontaneous Translational Fusion of Bacillus cereus PlcR and PapR Activates Transcription of PlcR-Dependent Genes in Bacillus anthracis via Binding with a Specific Palindromic Sequence

    OpenAIRE

    Pomerantsev, Andrei P; Pomerantseva, Olga M.; Stephen H Leppla

    2004-01-01

    Transformation of Bacillus anthracis with plasmid pUTE29-plcR-papR carrying the native Bacillus cereus plcR-papR gene cluster did not activate expression of B. anthracis hemolysin genes, even though these are expected to be responsive to activation by the global regulator PlcR. To further characterize the action of PlcR, we examined approximately 3,000 B. anthracis transformants containing pUTE29-plcR-papR and found a single hemolytic colony. The hemolytic strain contained a plasmid having a ...

  19. Formal verification of complex properties on PLC programs

    CERN Document Server

    Darvas, D; Voros, A; Bartha, T; Blanco Vinuela, E; Gonzalez Suarez, V M

    2014-01-01

    Formal verification has become a recommended practice in the safety-critical application areas. However, due to the complexity of practical control and safety systems, the state space explosion often prevents the use of formal analysis. In this paper we extend our former verification methodology with effective property preserving reduction techniques. For this purpose we developed general rule-based reductions and a customized version of the Cone of Influence (COI) reduction. Using these methods, the verification of complex requirements formalised with temporal logics (e.g. CTL, LTL) can be orders of magnitude faster. We use the NuSMV model checker on a real-life PLC program from CERN to demonstrate the performance of our reduction techniques.

  20. Coded Adaptive Linear Precoded Discrete Multitone Over PLC Channel

    CERN Document Server

    Muhammad, Fahad Syed; Hélard, Jean-François; Crussière, Matthieu

    2008-01-01

    Discrete multitone modulation (DMT) systems exploit the capabilities of orthogonal subcarriers to cope efficiently with narrowband interference, high frequency attenuations and multipath fadings with the help of simple equalization filters. Adaptive linear precoded discrete multitone (LP-DMT) system is based on classical DMT, combined with a linear precoding component. In this paper, we investigate the bit and energy allocation algorithm of an adaptive LP-DMT system taking into account the channel coding scheme. A coded adaptive LPDMT system is presented in the power line communication (PLC) context with a loading algorithm which accommodates the channel coding gains in bit and energy calculations. The performance of a concatenated channel coding scheme, consisting of an inner Wei's 4-dimensional 16-states trellis code and an outer Reed-Solomon code, in combination with the proposed algorithm is analyzed. Theoretical coding gains are derived and simulation results are presented for a fixed target bit error ra...

  1. British Nuclear Fuels PLC: report and accounts 1989-90

    International Nuclear Information System (INIS)

    Members of the Energy Select Committee put questions to representatives of British Nuclear Fuels PLC (BNFL) about the annual report and accounts 1989-90. Questions concerned the late publication of the accounts, BNFLs role in the downfall of the nuclear privatisation, government assistance to the nuclear industry, the price BNFL charges for fuel reprocessing and the process of laser isotope separation of uranium. The committee also asked about the rate of return in BNFL's assets as a percentage, Sellafield's potential as a site for a deep repository for radioactive wastes and the 6000 boreholes that MREX will drill at Sellafield. The commercial case for reprocessing is made. Feasibility studies for possible new reactions at Sellafield and Chapel Cross have been carried out. On the whole the Energy Committee were satisfied with the replies from BNFL. (UK)

  2. PLC-Based Pressure Control in Multi-Pump Applications

    Directory of Open Access Journals (Sweden)

    Vodovozov Valery

    2015-12-01

    Full Text Available The paper is devoted to the centrifugal pumps represented the most popular type of pumping equipment used in different areas. The pressure control approach for variable speed driven (VSD parallel connected centrifugal pumps is reported. The goal of the study is optimization of some quality indices, such as efficiency, consumed power, productivity, energy carrier temperature, heat irradiation, etc. One of them – efficiency – has been studied in the paper more carefully. The mathematical model of pumping process is discussed and a vector-matrix description of the multi-pump application is given. The program-based pressure control system is developed which productivity is changed by regulating the number of working pumps. The paper introduces new pressure control algorithms based on the working point estimation intended for programmable logical controllers (PLC. Experiments prove correctness of the offered methodology.

  3. Amersham plc UK Replacement Dose Record Keeping System (DRS2)

    International Nuclear Information System (INIS)

    The Dose Record Keeping System used for Amersham plc Classified persons in the UK has recently been replaced. This paper describes the system developed, to satisfy the UK legislation (IRR99 [1], RADS3 [2]) and allow the Radiation Protection Advisers quick and easy access to relevant information. The system has a fully featured security structure, allowing different individuals to have different levels of access and is run across four sites in the UK. The number of users at any one time is unlimited and the system is designed in such a way as to be fully expandable to any size of operation. An audit trail is employed to allow the tracking of all actions, from who logged on to who issued a dosimeter. All different components of dose are accounted for and the system is flexible enough to incorporate new dose levels / limits should they be introduced in the future. Historical data from the previous system has been migrated allowing seventeen years of dose information to be called onto the screen instantly. The entire dose history, down to individual dosimeter measurements, can be called up at any time. Operational information can also be stored along side Dose Record entries to assist the Radiation Protection Advisers in their duties (e.g. activity in urine, electronic dosimeter readings). The project was completed in close collaboration with a third party contractor and Amersham plc holds the Intellectual Property Rights (IPR). The architecture employed uses an Oracle database with a Windows XP application front end. The final developed system is fully configurable and has proved an invaluable tool for both the Radiation Protection Adviser and the Amersham Dosimetry Service. (Author) 2 refs

  4. Implementation of a Fractional Model-Based Fault Detection Algorithm into a PLC Controller

    Science.gov (United States)

    Kopka, Ryszard

    2014-12-01

    This paper presents results related to the implementation of model-based fault detection and diagnosis procedures into a typical PLC controller. To construct the mathematical model and to implement the PID regulator, a non-integer order differential/integral calculation was used. Such an approach allows for more exact control of the process and more precise modelling. This is very crucial in model-based diagnostic methods. The theoretical results were verified on a real object in the form of a supercapacitor connected to a PLC controller by a dedicated electronic circuit controlled directly from the PLC outputs.

  5. PLC PROGRAMLAMA EĞİTİMİNDE PC_SIMU YAZILIMININ KULLANIMI

    OpenAIRE

    ENGİN UFUK ERGÜL; CENK GEZEGİN

    2013-01-01

    PLC (Programlanabilir Lojik Denetleyici)’ lerin son yıllarda artan kullanımına paralel olarak bu konuda yetişmiş bilgi ve beceriye sahip teknik personele olan talep de artmaktadır. PLC’ lerin ek donanımlarının ve PLC içeren deney setlerinin fiyatları oldukça yüksektir. Bu yüzden eğitim kurumları PLC eğitimi konusunda öğrencilere tüm imkânları sağlamakta yetersiz kalmaktadır. Ayrıca gerçek sistemlere benzeyen PLC kontrollü proseslerin boyutları bir laboratuara sığdırılamayacak kadar büyük ve m...

  6. Modelling and Formal Verification of Timing Aspects in Large PLC Programs

    CERN Document Server

    Fernandez Adiego, B; Blanco Vinuela, E; Tournier, J-C; Gonzalez Suarez, V M; Blech, J O

    2014-01-01

    One of the main obstacle that prevents model checking from being widely used in industrial control systems is the complexity of building formal models out of PLC programs, especially when timing aspects need to be integrated. This paper brings an answer to this obstacle by proposing a methodology to model and verify timing aspects of PLC programs. Two approaches are proposed to allow the users to balance the trade-off between the complexity of the model, i.e. its number of states, and the set of specifications possible to be verified. A tool supporting the methodology which allows to produce models for different model checkers directly from PLC programs has been developed. Verification of timing aspects for real-life PLC programs are presented in this paper using NuSMV.

  7. 77 FR 20987 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-04-09

    ..., Amendment 39-16970 (77 FR 13485, March 7, 2012), currently requires inspecting the front combustion liner...; AD 2012-04-14] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc Turbofan Engines...

  8. Aero Repair and Overhaul Services at Rolls Royce Group Plc: Application of Activity Based Costing

    OpenAIRE

    Vaithiyanathan, Shivany

    2005-01-01

    In this report an analysis has been carried out to study the application of Activity Based Costing (ABC) to the proposed repair and overhaul process at Rolls Royce Group Plc after the implementation of the aftermarket business policies.

  9. Performance analysis of the PLC (Power Line Communication) in medium voltage electrical networks; Analise de desempenho de sistemas PLC em redes eletricas de media tensao

    Energy Technology Data Exchange (ETDEWEB)

    Mota, A.A.; Paleta, R. [Pontificia Universidade Catolica de Campinas (PUC-Campinas), SP (Brazil)], E-mail: amota@puc-campinas.edu.br; Mota, L.T.M.; Ricardo, R.A. [Indelmatec Engenharia, Campinas, SP (Brazil)], E-mail: mota@indelmatec.com.br

    2009-07-01

    Nowadays, the information access in communication networks is widely explored due to the increase of Internet users. In this context, the PLC (Power Line Communication) technology is an alternative for data transmission. This technology is based on the usage of transmission/distribution power lines for data transmission. However there are some problems related to the usage of this technology: adequate data transmission rates and generation of acceptable levels of electromagnetic interference (EMI). This work had the objective of studying the performance of PLC systems in medium voltage electrical networks, through the assess of data transmission rates and the generated EMI. Tests were carried out in a test field that corresponded to a medium voltage electrical network and the obtained results show that, under some circumstances, the PLC system does not reach the existent technical recommendations. (author)

  10. Gnb isoforms control a signaling pathway comprising Rac1, Plcβ2, and Plcβ3 leading to LFA-1 activation and neutrophil arrest in vivo.

    Science.gov (United States)

    Block, Helena; Stadtmann, Anika; Riad, Daniel; Rossaint, Jan; Sohlbach, Charlotte; Germena, Giulia; Wu, Dianqing; Simon, Scott I; Ley, Klaus; Zarbock, Alexander

    2016-01-21

    Chemokines are required for leukocyte recruitment and appropriate host defense and act through G protein-coupled receptors (GPCRs), which induce downstream signaling leading to integrin activation. Although the α and β subunits of the GPCRs are the first intracellular molecules that transduce signals after ligand binding and are therefore indispensable for downstream signaling, relatively little is known about their contribution to lymphocyte function-associated antigen 1 (LFA-1) activation and leukocyte recruitment. We used knockout mice and short hairpin RNA to knock down guanine nucleotide binding protein (GNB) isoforms (GNB1, GNB2, GNB4, and GNB5) in HL60 cells and primary murine hematopoietic cells. Neutrophil function was assessed by using intravital microscopy, flow chamber assays, and chemotaxis and biochemistry studies. We unexpectedly discovered that all expressed GNB isoforms are required for LFA-1 activation. Their downregulation led to a significant impairment of LFA-1 activation, which was demonstrated in vitro and in vivo. Furthermore, we showed that GPCR activation leads to Ras-related C3 botulinum toxin substrate 1 (Rac1)-dependent activation of both phospholipase C β2 (Plcβ2) and Plcβ3. They act nonredundantly to produce inositol triphosphate-mediated intracellular Ca(2+) flux and LFA-1 activation that support chemokine-induced arrest in vivo. In a complex inflammatory disease model, Plcβ2-, Plcβ3-, or Rac1-deficient mice were protected from lipopolysaccharide-induced lung injury. Taken together, we demonstrated that all Gnb isoforms are required for chemokine-induced downstream signaling, and Rac1, Plcβ2, and Plcβ3 are critically involved in integrin activation and leukocyte arrest. PMID:26468229

  11. Enhancements of G3-PLC Technology for Smart-Home/Building Applications

    OpenAIRE

    Luca Di Bert; Salvatore D'Alessandro; Tonello, Andrea M.

    2013-01-01

    To enable the smart grid concept, it is fundamental to consider the in-home/building context where, beside the conventional home networking services, home automation and smart energy management services have to be offered. In this paper, we consider the in-home/building scenario, for which we propose a convergent network architecture to enhance the performance of the narrowband power line communication (PLC) G3-PLC technology through its integration with an Ethernet-based network. To this end...

  12. 78 FR 11976 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2013-02-21

    ..., Amendment 39-16724 (76 FR 40217, July 8, 2011) and adding the following new AD: 2013-04-01 Rolls-Royce plc... (76 FR 40217, July 8, 2011). (c) Applicability This AD applies to Rolls-Royce plc (RR) RB211-524D4-19... publication as of August 12, 2011, (76 FR 40217, July 8, 2011). We must receive any comments on this AD...

  13. 76 FR 65136 - Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines

    Science.gov (United States)

    2011-10-20

    ...-07, Amendment 39-166679 (76 FR 24793, May 3, 2011), and adding the following new AD: Rolls-Royce plc..., Amendment 39-16669 (76 FR 24793, May 3, 2011). (c) Applicability This AD applies to Rolls-Royce plc (RR...-09-07, Amendment 39-16669 (76 FR 24793, May 3, 2011), for all RR model RB211-524G2-T-19,...

  14. Corporate Social Responsibility and Organizational Profitability: An Empirical Investigation of United Bank for Africa (UBA) Plc.

    OpenAIRE

    Oyetayo O. Folajin; Oluwaseun T. Ibitoye; A.T. Dunsin

    2014-01-01

    This study investigated the impact of Corporate Social Responsibility on bank profitability with particular reference to United Bank for Africa (UBA) Plc. The study used annual reports of United Bank for Africa (UBA) Plc. Data used include corporate social responsibility expenditure and profit after tax for the period of 2006-2012. Data relating to cost/investment/expenditure as the case may be for the bank on corporate social responsibility and profitability was used to construct ordinary le...

  15. Biofilm Formation by Bacillus cereus Is Influenced by PlcR, a Pleiotropic Regulator

    OpenAIRE

    Hsueh, Yi-Huang; Somers, Eileen B.; Lereclus, Didier; Wong, Amy C. Lee

    2006-01-01

    The ΔplcR mutant of Bacillus cereus strain ATCC 14579 developed significantly more biofilm than the wild type and produced increased amounts of biosurfactant. Biosurfactant production is required for biofilm formation and may be directly or indirectly repressed by PlcR, a pleiotropic regulator. Coating polystyrene plates with surfactin, a biosurfactant from Bacillus subtilis, rescued the deficiency in biofilm formation by the wild type.

  16. Enhanced Operation of Electricity Distribution Grids Through Smart Metering PLC Network Monitoring, Analysis and Grid Conditioning

    OpenAIRE

    Iker Urrutia; Pablo Angueira; Xabier Osorio; Aitor Arzuaga; Alberto Sendin; Iñigo Berganza

    2013-01-01

    Low Voltage (LV) electricity distribution grid operations can be improved through a combination of new smart metering systems’ capabilities based on real time Power Line Communications (PLC) and LV grid topology mapping. This paper presents two novel contributions. The first one is a new methodology developed for smart metering PLC network monitoring and analysis. It can be used to obtain relevant information from the grid, thus adding value to existing smart metering deployments and facili...

  17. Developing a Novel USB-PLC Controller for a Mechatronics Cloud Laboratory

    OpenAIRE

    Wen-Jye Shyr; Chia-Ming Lin

    2013-01-01

    This study proposes the development and implementation of a novel Universal Serial Bus (USB)‐Programmable Logic Controller (PLC), called a USB‐PLC controller, for a mechatronics cloud laboratory. The aim of a mechatronics cloud laboratory is to provide state of the art research quality equipment to students, allowing them to conduct hands‐on experiments via the Internet. One objective of the cloud laboratory is to not only provide equipment for conducting set experiments, but also to provide ...

  18. Cbl Suppresses B Cell Receptor–Mediated Phospholipase C (Plc)-γ2 Activation by Regulating B Cell Linker Protein–Plc-γ2 Binding

    OpenAIRE

    Yasuda, Tomoharu; Maeda, Akito; Kurosaki, Mari; Tezuka, Tohru; Hironaka, Katsunori; Yamamoto, Tadashi; Kurosaki, Tomohiro

    2000-01-01

    Accumulating evidence indicates that the Cbl protein plays a negative role in immune receptor signaling; however, the mode of Cbl action in B cell receptor (BCR) signaling still remains unclear. DT40 B cells deficient in Cbl showed enhanced BCR-mediated phospholipase C (PLC)-γ2 activation, thereby leading to increased apoptosis. A possible explanation for the involvement of Cbl in PLC-γ2 activation was provided by findings that Cbl interacts via its Src homology 2 (SH2) domain with B cell lin...

  19. A formal safety analysis for PLC software-based safety critical system using Z

    International Nuclear Information System (INIS)

    This paper describes a formal safety analysis technique which is demonstrated by performing empirical formal safety analysis with the case study of beamline hutch door Interlock system that is developed by using PLC (Programmable Logic Controller) systems at the Pohang Accelerator Laboratory. In order to perform formal safety analysis, we have built the Z formal specifications representation from user requirement written in ambiguous natural language and target PLC ladder logic, respectively. We have also studied the effective method to express typical PLC timer component by using specific Z formal notation which is supported by temporal history. We present a formal proof technique specifying and verifying that the hazardous states are not introduced into ladder logic in the PLC-based safety critical system. And also, we have found that some errors or mismatches in user requirement and final implemented PLC ladder logic while analyzing the process of the consistency and completeness of Z translated formal specifications. In the case of relatively small systems like Beamline hutch door interlock system, a formal safety analysis including explicit proof is highly recommended so that the safety of PLC-based critical system may be enhanced and guaranteed. It also provides a helpful benefits enough to comprehend user requirement expressed by ambiguous natural language

  20. Developing a PLC-friendly state machine model: lessons learned

    Science.gov (United States)

    Pessemier, Wim; Deconinck, Geert; Raskin, Gert; Saey, Philippe; Van Winckel, Hans

    2014-07-01

    Modern Programmable Logic Controllers (PLCs) have become an attractive platform for controlling real-time aspects of astronomical telescopes and instruments due to their increased versatility, performance and standardization. Likewise, vendor-neutral middleware technologies such as OPC Unified Architecture (OPC UA) have recently demonstrated that they can greatly facilitate the integration of these industrial platforms into the overall control system. Many practical questions arise, however, when building multi-tiered control systems that consist of PLCs for low level control, and conventional software and platforms for higher level control. How should the PLC software be structured, so that it can rely on well-known programming paradigms on the one hand, and be mapped to a well-organized OPC UA interface on the other hand? Which programming languages of the IEC 61131-3 standard closely match the problem domains of the abstraction levels within this structure? How can the recent additions to the standard (such as the support for namespaces and object-oriented extensions) facilitate a model based development approach? To what degree can our applications already take advantage of the more advanced parts of the OPC UA standard, such as the high expressiveness of the semantic modeling language that it defines, or the support for events, aggregation of data, automatic discovery, ... ? What are the timing and concurrency problems to be expected for the higher level tiers of the control system due to the cyclic execution of control and communication tasks by the PLCs? We try to answer these questions by demonstrating a semantic state machine model that can readily be implemented using IEC 61131 and OPC UA. One that does not aim to capture all possible states of a system, but rather one that attempts to organize the course-grained structure and behaviour of a system. In this paper we focus on the intricacies of this seemingly simple task, and on the lessons that we

  1. Bacillus cereus Fnr binds a [4Fe-4S] cluster and forms a ternary complex with ResD and PlcR

    Directory of Open Access Journals (Sweden)

    Esbelin Julia

    2012-06-01

    Full Text Available Abstract Background Bacillus cereus is a facultative anaerobe that causes diarrheal disease in humans. Diarrheal syndrome may result from the secretion of various virulence factors including hemolysin BL and nonhemolytic enterotoxin Nhe. Expression of genes encoding Hbl and Nhe is regulated by the two redox systems, ResDE and Fnr, and the virulence regulator PlcR. B. cereus Fnr is a member of the Crp/Fnr family of iron-sulfur (Fe-S proteins. Only its apo-form has so far been studied. A major goal in deciphering the Fnr-dependent regulation of enterotoxin genes is thus to obtain and characterize holoFnr. Results Fnr has been subjected to in vitro Fe-S cluster reconstitution under anoxic conditions. UV-visible and EPR spectroscopic analyses together with the chemical estimation of the iron content indicated that Fnr binds one [4Fe-4S]2+ cluster per monomer. Atmospheric O2 causes disassembly of the Fe-S cluster, which exhibited a half-life of 15 min in air. Holo- and apoFnr have similar affinities for the nhe and hbl promoter regions, while holoFnr has a higher affinity for fnr promoter region than apoFnr. Both the apo- and holo-form of Fnr interact with ResD and PlcR to form a ternary complex. Conclusions Overall, this work shows that incorporation of the [4Fe-4S]2+ cluster is not required for DNA binding of Fnr to promoter regions of hbl and nhe enterotoxin genes or for the formation of a ternary complex with ResD and PlcR. This points to some new unusual properties of Fnr that may have physiological relevance in the redox regulation of enterotoxin gene regulation.

  2. ENCODE data at the ENCODE portal.

    Science.gov (United States)

    Sloan, Cricket A; Chan, Esther T; Davidson, Jean M; Malladi, Venkat S; Strattan, J Seth; Hitz, Benjamin C; Gabdank, Idan; Narayanan, Aditi K; Ho, Marcus; Lee, Brian T; Rowe, Laurence D; Dreszer, Timothy R; Roe, Greg; Podduturi, Nikhil R; Tanaka, Forrest; Hong, Eurie L; Cherry, J Michael

    2016-01-01

    The Encyclopedia of DNA Elements (ENCODE) Project is in its third phase of creating a comprehensive catalog of functional elements in the human genome. This phase of the project includes an expansion of assays that measure diverse RNA populations, identify proteins that interact with RNA and DNA, probe regions of DNA hypersensitivity, and measure levels of DNA methylation in a wide range of cell and tissue types to identify putative regulatory elements. To date, results for almost 5000 experiments have been released for use by the scientific community. These data are available for searching, visualization and download at the new ENCODE Portal (www.encodeproject.org). The revamped ENCODE Portal provides new ways to browse and search the ENCODE data based on the metadata that describe the assays as well as summaries of the assays that focus on data provenance. In addition, it is a flexible platform that allows integration of genomic data from multiple projects. The portal experience was designed to improve access to ENCODE data by relying on metadata that allow reusability and reproducibility of the experiments. PMID:26527727

  3. Software control of the Advanced Technology Solar Telescope enclosure PLC hardware using COTS software

    Science.gov (United States)

    Borrowman, Alastair J.; de Bilbao, Lander; Ariño, Javier; Murga, Gaizka; Goodrich, Bret; Hubbard, John R.; Greer, Alan; Mayer, Chris; Taylor, Philip

    2012-09-01

    As PLCs evolve from simple logic controllers into more capable Programmable Automation Controllers (PACs), observatories are increasingly using such devices to control complex mechanisms1, 2. This paper describes use of COTS software to control such hardware using the Advanced Technology Solar Telescope (ATST) Common Services Framework (CSF). We present the Enclosure Control System (ECS) under development in Spain and the UK. The paper details selection of the commercial PLC communication library PLCIO. Implemented in C and delivered with source code, the library separates the programmer from communication details through a simple API. Capable of communicating with many types of PLCs (including Allen-Bradley and Siemens) the API remains the same irrespective of PLC in use. The ECS is implemented in Java using the observatory's framework that provides common services for software components. We present a design following a connection-based approach where all components access the PLC through a single connection class. The link between Java and PLCIO C library is provided by a thin Java Native Interface (JNI) layer. Also presented is a software simulator of the PLC based upon the PLCIO Virtual PLC. This creates a simulator operating below the library's API and thus requires no change to ECS software. It also provides enhanced software testing capabilities prior to hardware becoming available. Results are presented in the form of communication timing test data, showing that the use of CSF, JNI and PLCIO provide a control system capable of controlling enclosure tracking mechanisms, that would be equally valid for telescope mount control.

  4. Realizace úzkopásmových PLC modemů

    OpenAIRE

    Kubíček, Lukáš

    2012-01-01

    Diplomová práce se věnuje úzkopásmové technologii přenosu dat el. sítí NN (PLC) a~možnostem jejího využití v senzorových sítích. Zabývá se jak strukturou a základními vlastnostmi PLC standardu, tak možnostmi jeho uplatnění v praxi. Hlavní částí této práce je realizace PLC sítě pomocí IO ST7570 firmy STMicroelectronics. Jako příklad praktického využití PLC technologie je tato síť připojena k počítači, ve kterém jsou data ze senzoru (wattmetru) připojenému k PLC modemu ukládána pomocí aplikace ...

  5. PLC ? datové přenosy v elektrorozvodné síti

    OpenAIRE

    Páter, David

    2007-01-01

    Cílem této diplomové práce je seznámit čtenáře s konceptem datových přenosů v elektrorozvodné síti ? PLC (Powerline Communication), provést analýzu současného stavu v PLC oblasti ve světě a v České republice, vyzkoušet možnosti PLC v praxi, identifikovat přednosti a omezení PLC a srovnat PLC s konkurenčními komunikačními technologiemi v České republice. Za účelem dosažení stanovených cílů je diplomová práce členěna na dvě hlavní části ? teoretickou a praktickou. Teoretická část je založena př...

  6. PLC/DTAM Software Programs for Pumping Instrumentation and Control Skid X

    International Nuclear Information System (INIS)

    This document describes the software programs for the Allen-Bradley SLC 500 programmable logic controller (PLC) and the Allen-Bradley DTAM PLUS operator interface module used on Pumping Instrumentation and Control (PIC) skid ''X''. The software programs for the SLC 500 and DTAM Plus are based on the core programs provided by Allen-Bradley. The PLC and DTAM software programs on skid ''D'' for SX-104 are the baseline programs. These baselines will be tailored for each individual BY-farm skid. An Acceptance Test Procedure (ATP) and an Operational Test Procedure (OTP) verify that the software programs meet the specific requirements for BY-105 pumping. This document represents the final PLC and DTAM programs for PIC skid ''X'' at BY-105. These programs were printed out after the performance of the OTP. The OTP acts as the final qualification test for the software programs. Functional requirements and details of the PLC ladder logic are described in this document. The final programs entered into the PLC and DTAM Plus are included as Appendices to this document

  7. PLC and DTAM Software Programs for Pumping Instrumentation and Control Skid N

    International Nuclear Information System (INIS)

    This document describe the software programs for the Programmable Logic Controller and the Datable Access Module for Pumping Instrumentation and Control skid ''N''. The Appendices contains copies of the printouts of these software programs. This document describes the software programs for the Allen-Bradley SLC 500 programmable logic controller (PLC) and the Allen-Bradley DTAM PLUS operator interface module used on Pumping Instrumentation and Control (PIC) skid ''N''. The software programs for the SLC 500 and DTAM Plus are based on the core programs provided by Allen-Bradley. The PLC and DTAM software programs on skid ''D'' for SX-104 are the baseline programs. These baselines have been tailored for U-farm skids. The skid ''N'' program for U-109 is similar to the skid ''M'' program for U-102. An Acceptance Test Procedure (ATP) and an Operational Test Procedure (OTP) verify that the software programs meet the specific requirements for U-109 pumping. This document represents the final PLC and DTAM programs for PIC skid ''N'' at U-109. These programs were printed out after the performance of the OTP. The OTP acts as the final qualification test for the software programs. Functional requirements and details of the PLC ladder logic are described in this document. The final programs entered into the PLC and DTAM Plus are included as Appendices to this document

  8. NB-PLC channel modelling with cyclostationary noise addition & OFDM implementation for smart grid

    Science.gov (United States)

    Thomas, Togis; Gupta, K. K.

    2016-03-01

    Power line communication (PLC) technology can be a viable solution for the future ubiquitous networks because it provides a cheaper alternative to other wired technology currently being used for communication. In smart grid Power Line Communication (PLC) is used to support communication with low rate on low voltage (LV) distribution network. In this paper, we propose the channel modelling of narrowband (NB) PLC in the frequency range 5 KHz to 500 KHz by using ABCD parameter with cyclostationary noise addition. Behaviour of the channel was studied by the addition of 11KV/230V transformer, by varying load location and load. Bit error rate (BER) Vs signal to noise ratio SNR) was plotted for the proposed model by employing OFDM. Our simulation results based on the proposed channel model show an acceptable performance in terms of bit error rate versus signal to noise ratio, which enables communication required for smart grid applications.

  9. The upgrade of an educational observatory control system with a PLC-based architecture

    Science.gov (United States)

    Baldini, V.; Cirami, R.; Coretti, I.; Di Marcantonio, P.; Galeotta, S.; Iafrate, G.; Mannetta, M.; Santin, P.

    2014-07-01

    A Celestron C14 telescope equipped with a robotic Paramount ME equatorial mount is being used for public outreach at the Basovizza site of the INAF-Astronomical Observatory of Trieste. Although the telescope could be fully remotely controlled, the control of the instrumentations and the movement of the main motor of the dome requires the physical presence of an operator. To overcome this limitation the existing control system has been upgraded using a Beckhoff PLC to allow the remote control of the whole instrumentation, including the management of the newly installed weather sensor and the access to the telescope area. Exploiting the decentralization features typical of a PLC based solution, the PLC modules are placed in two different racks, according to the function to be controlled. A web interface is used for the communication between the user and the instrumentation. The architecture of this control system will be presented in detail in this paper.

  10. Bringing Automated Model Checking to PLC Program Development - A CERN Case Study

    CERN Document Server

    Fernandez Adiego, B; Tournier, J-C; Blanco Vinuela, E; Gonzalez Suarez, V M

    2014-01-01

    Verification of critical software is a high priority but a challenging task for industrial control systems. Model checking appears to be an appropriate approach for this purpose. However, this technique is not widely used in industry yet, due to some obstacles. The main obstacles encountered when trying to apply formal verification techniques at industrial installations are the difficulty of creating models out of PLC programs and defining formally the specification requirements. In addition, models produced out of real-life programs have a huge state space, thus preventing the verification due to performance issues. Our work at CERN (European Organization for Nuclear Research) focuses on developing efficient automatic verification methods for industrial critical installations based on PLC (Programmable Logic Controller) control systems. In this paper, we present a tool generating automatically formal models out of PLC code. The tool implements a general methodology which can support several input languages, ...

  11. Technical considerations for the development of an engineering safety features control system with PLC

    International Nuclear Information System (INIS)

    Technical considerations are summarized for the development of an ESFCS(Engineered Safety Features Control System) with PLC (Programmable Logic Controller). The ESFCS is required for the mitigation of plant accident conditions and therefore developed in conformance with the design requirements applied to the safety critical system. The design of ESFCS primarily considered its safety, and the system has an architecture that will be able to minimize spurious actuation. The PLC based functional distribution and redundant design features are adopted, and the fieldbus is applied in the communication of information and control signals between PLC processors. It is expected that the ESFCS will have several advanced design features compared with the conventional systems supplied by foreign vendors

  12. Mitigation of Noise in OFDM Based Plc System Using Filter Kernel Design

    Directory of Open Access Journals (Sweden)

    Nisha G Krishnan

    2015-09-01

    Full Text Available Power line communication is a technology that transforms power line in to pathway for conveyance of broadband data. It is cost less than other communication approach and for better bandwidth efficiency OFDM based PLC system is used. In real PLC environment some electrical appliances will produce noise. To mitigate this noise filter kernel design is used, so periodic impulsive noise and Gaussian noises are removed from PLC communication system by using this filter kernel design. MATLAB is used for the simulation and the result shows that filter kernel is simple and effective noise mitigation technique. Further in future, interference due to obstacles also wants to be mitigated for the better data transmission without noise.

  13. 77 FR 10355 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2012-02-22

    ... (68 FR 49344, August 18, 2003) and adding the following new AD: 2012-04-01 Rolls-Royce plc: Amendment... (68 FR 49344, August 18, 2003). (c) Applicability This AD applies to Rolls-Royce plc (RR) RB211-Trent...) 2003-16- 18, amendment 39-13271 (68 FR 49344, August 18, 2003). That AD applies to the...

  14. 77 FR 21588 - Hart and Cooley, Inc., A Subsidiary of Tomkins, PLC Including On-Site Leased Workers from...

    Science.gov (United States)

    2012-04-10

    ... of determination was published in the Federal Register on April 23, 2010 (75 FR 21354). At the... Employment and Training Administration Hart and Cooley, Inc., A Subsidiary of Tomkins, PLC Including On- Site... subsidiary of Tomkins, PLC, including on-site leased workers from Reliable, Masiello Employment...

  15. File list: Oth.Plc.10.AllAg.Induced_trophoblast_stem_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available Oth.Plc.10.AllAg.Induced_trophoblast_stem_cells mm9 TFs and others Placenta Induced trophoblast stem... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/Oth.Plc.10.AllAg.Induced_trophoblast_stem_cells.bed ...

  16. File list: InP.Plc.10.AllAg.Induced_trophoblast_stem_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Plc.10.AllAg.Induced_trophoblast_stem_cells mm9 Input control Placenta Induced trophoblast stem... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Plc.10.AllAg.Induced_trophoblast_stem_cells.bed ...

  17. File list: InP.Plc.20.AllAg.Induced_trophoblast_stem_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available InP.Plc.20.AllAg.Induced_trophoblast_stem_cells mm9 Input control Placenta Induced trophoblast stem... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/InP.Plc.20.AllAg.Induced_trophoblast_stem_cells.bed ...

  18. File list: NoD.Plc.10.AllAg.Induced_trophoblast_stem_cells [Chip-atlas[Archive

    Lifescience Database Archive (English)

    Full Text Available NoD.Plc.10.AllAg.Induced_trophoblast_stem_cells mm9 No description Placenta Induced trophoblast stem... cells http://dbarchive.biosciencedbc.jp/kyushu-u/mm9/assembled/NoD.Plc.10.AllAg.Induced_trophoblast_stem_cells.bed ...

  19. A Practical Applications of Virtual PLC using LabVIEW Software

    Directory of Open Access Journals (Sweden)

    Mohammad K. Abuzalata

    2013-05-01

    Full Text Available In this study thirteen different functions (VIs are designed and tested .These include, single input single output, single input two outputs, latch outputs, timer, counter, logic function, less, greater and equal functions, XOR function, compound function and shift register. At the end of the study, for illustration purposes, the 7-day tea maker, electro-pneumatic drive system and their simulation were developed and tested. Results of experiment show complete coincidence between the PLC-based control and Virtual PLC-based program results.

  20. An Integrated Software Development Framework for PLC and FPGA based Digital I and Cs

    International Nuclear Information System (INIS)

    NuDE 2.0 (Nuclear Development Environment) is a model-based software development environment for safety- critical digital systems in nuclear power plants. It makes possible to develop PLC-based systems as well as FPGA-based systems simultaneously from the same requirement or design specifications. The case study showed that the NuDE 2.0 can be adopted as an effective method of bridging the gap between the existing PLC and upcoming FPGA-based developments as well as a means of gaining diversity

  1. Development Research for Cramo Plc Annual Report to Meet the Requirements of IR Communications

    OpenAIRE

    Hirvonen, Mirjam

    2010-01-01

    The main objective of the research is to attain feedback on the 2009 Annual Report from selected stakeholders that could help to develop the annual report of the commissioning company, Cramo Plc, for the year 2011. Other objectives are to find out what status the annual report has among the sample and how it is used. The author worked at Cramo Plc as assistant trainee during the research process. The annual report is a key communication tool of a public limited company; it is designed...

  2. Development of an OPC and PLC Based Remote-Access Laboratory: A Synchronous Motor Control Experiment

    OpenAIRE

    VADI, Seyfettin

    2015-01-01

    In this study, OPC and PLC based remote-access laboratory has been developed for synchronous motor control experiment. The monitoring and control of the parameters of synchronous motor has been realized using GPRS and Profi-Lab OPC Server through the visual programming language. In addition, S7-1200 CPU 1214 DC/DC/DC series PLC was used for control of the system. The motor parameters monitored in real-time by the user to visually is shown the effect of any change in the parameters of the moto...

  3. AB Nordea Bank Finland Plc Lietuvos skyriaus darbdavio vidinio įvaizdžio stiprinimas

    OpenAIRE

    Stundytė, Inga

    2009-01-01

    Ši tema pasirinkta siekiant išanalizuoti Nordea banko esamą darbdavio įvaizdį, bei išaiškinti darbuotojų pasitenkinimo juo lygį. Nagrinėti darbdavio įvaizdį svarbu dėl to, kad egzistuoja ryšis tarp pasitenkinimo darbdaviu bei įsipareigojimo organizacijai. Darbo tikslas: Parengti rekomendacijas AB Nordea Bank Finland Plc Lietuvos skyriaus vidinio darbdavio įvaizdžio stiprinimui. Darbo uždaviniai: Nustatyti veiksnius, neigiamai įtakojančius AB Nordea Bank Finland Plc Lietuvos skyriaus ...

  4. The Application of PLC (Powerline Communications) in Brazil in the regulatory viewpoint; A aplicacao do PLC no Brasil do ponto de vista regulatorio

    Energy Technology Data Exchange (ETDEWEB)

    Vieira, Daniel; Silva Filho, Armando [Agencia Nacional de Energia Eletrica (ANEEL), Brasilia, DF (Brazil). Superintendencia de Regulacao dos Servicos de Distribuicao

    2010-07-01

    The Powerline Communications technology - PLC allows transmission of data, voice, image and access to high speed internet by the grid, so that a power plug can also be an access point to the network communication. Considering that the electric grid reaches the vast majority of households, there is great potential in using this technology as a tool to achieve the reality of digital inclusion in the country. However, its implementation must be accompanied by effective regulation, both through policies with respect to the conditions of use of radio frequencies, as in regulating the use of distribution networks of electricity as a means of transport for communication signal. In this context, the present article deals with the fundamental rules governing the application of PLC technology from the standpoint of the distributors and consumers of electricity, with attention to quality assurance of electrical energy and facility security.

  5. 76 FR 2605 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2011-01-14

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines AGENCY: Federal Aviation...

  6. 77 FR 56760 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-09-14

    ... April 11, 2000 (65 FR 19477-78). Authority for This Rulemaking Title 49 of the United States Code... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule;...

  7. 77 FR 39157 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-07-02

    ..., Amendment 39-16384 (75 FR 49368, August 13, 2010). (c) Applicability This AD applies to Rolls-Royce plc (RR..., 2010, we issued AD 2010-16-07, Amendment 39-16384 (75 FR 49368, August 13, 2010), for RR model RB211... Since we issued AD 2010-16-07 (75 FR 49368, August 13, 2010), RR determined that engines that are...

  8. 77 FR 32007 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-05-31

    ... FR 19477-78). Authority for This Rulemaking Title 49 of the United States Code specifies the FAA's... ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3... plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule;...

  9. 78 FR 6749 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2013-01-31

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic... Directives; Rolls-Royce plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT....

  10. Trust and Collaboration in PLC Teams: Teacher Relationships, Principal Support, and Collaborative Benefits

    Science.gov (United States)

    Hallam, Pamela R.; Smith, Henry R.; Hite, Julie M.; Hite, Steven J.; Wilcox, Bradley R.

    2015-01-01

    Professional learning communities (PLCs) are being recognized as effective in improving teacher collaboration and student achievement. Trust is critical in effectively implementing the PLC model, and the school principal is best positioned to influence school trust levels. Using five facets of trust, this research sought to clarify the impact of…

  11. 77 FR 2932 - Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines

    Science.gov (United States)

    2012-01-20

    ..., (73 FR 52201, September 9, 2008). (c) Applicability This AD applies to Rolls-Royce plc (RR) RB211... Federal Register on October 18, 2011 (76 FR 64283). That NPRM proposed to require for certain RB211-Trent... 12866, (2) Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...

  12. 76 FR 52288 - Airworthiness Directives; Rolls-Royce plc (RR) Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2011-08-22

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and (3) Will... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  13. Applying Hamming Code to Memory System of Safety Grade PLC (POSAFE-Q) Processor Module

    International Nuclear Information System (INIS)

    If some errors such as inverted bits occur in the memory, instructions and data will be corrupted. As a result, the PLC may execute the wrong instructions or refer to the wrong data. Hamming Code can be considered as the solution for mitigating this mis operation. In this paper, we apply hamming Code, then, we inspect whether hamming code is suitable for to the memory system of the processor module. In this paper, we applied hamming code to existing safety grade PLC (POSAFE-Q). Inspection data are collected and they will be referred for improving the PLC in terms of the soundness. In our future work, we will try to improve time delay caused by hamming calculation. It will include CPLD optimization and memory architecture or parts alteration. In addition to these hamming code-based works, we will explore any methodologies such as mirroring for the soundness of safety grade PLC. Hamming code-based works can correct bit errors, but they have limitation in multi bits errors

  14. A Research on Seamless Platform Change of Reactor Protection System From PLC to FPGA

    International Nuclear Information System (INIS)

    The PLC (Programmable Logic Controller) has been widely used to implement real-time controllers in nuclear RPSs (Reactor Protection Systems). Increasing complexity and maintenance cost, however, are now demanding more powerful and cost-effective implementation such as FPGA (Field-Programmable Gate Array). Abandoning all experience and knowledge accumulated over the decades and starting an all-new development approach is too risky for such safety-critical systems. This paper proposes an RPS software development process with a platform change from PLC to FPGA, while retaining all outputs from the established development. This paper transforms FBD designs of the PLC-based software development into a behaviorally-equivalent Verilog program, which is a starting point of a typical FPGA-based hardware development. We expect that the proposed software development process can bridge the gap between two software developing approaches with different platforms, such as PLC and FPGA. This paper also demonstrates its effectiveness using an example of a prototype version of a real-world RPS in Korea

  15. 77 FR 75831 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-12-26

    ... December 14, 2007 (72 FR 67568, November 29, 2007). (i) Rolls-Royce plc Service Bulletin No. RB.211-72-F117... incorporation by reference of certain other publications as of December 14, 2007 (72 FR 67568, November 29, 2007... 2007-24-09 (72 FR 67568, November 29, 2007) which corresponds with EASA AD 2007-0260R1. Our AD has...

  16. 75 FR 15321 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2010-03-29

    ... Register on January 5, 2010 (75 FR 264). That NPRM proposed to correct an unsafe condition for the... Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant...; AD 2010-06-14] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc RB211-Trent 800...

  17. 77 FR 13483 - Airworthiness Directives; Rolls-Royce plc (RR) Turbofan Engines

    Science.gov (United States)

    2012-03-07

    ...-07 (76 FR 24793, May 3, 2011), or, have Rolls-Royce plc revise Alert Service Bulletin (ASB) No. RB..., Amendment 39-16669 (76 FR 24793, May 3, 2011). That AD applies to the specified products. The NPRM published in the Federal Register on October 20, 2011 (76 FR 65136). That NPRM proposed to continue to...

  18. 77 FR 6668 - Airworthiness Directives; Rolls-Royce plc Turbofan Engines

    Science.gov (United States)

    2012-02-09

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have... plc Turbofan Engines AGENCY: Federal Aviation Administration (FAA), DOT. ACTION: Final rule;...

  19. Úzkopásmová PLC komunikace pro Smart Metering

    OpenAIRE

    Kubů, Jiří

    2016-01-01

    Tato bakalářská práce se zabývá úzkopásmovou PLC komunikací. V práci je popsán základní princip, rozdělení PLC komunikace, historie technologie, využívané komunikační standardy a rušení ovlivňující datovou komunikaci této technologie. V rámci této práce byly provedeny i experimentální měření v reálném prostředí s modemy od firmy Texas Instruments C2000 PLC TMDSPLCKITV4 a C2000 PLC TMDSPLCKIT-V3. Výsledky tohoto měření jsou reálné přenosové rychlosti. Dále byla vytvořena aplikace pro dálkový s...

  20. Automated Translation of Safety Critical Application Software Specifications into PLC Ladder Logic

    Science.gov (United States)

    Leucht, Kurt W.; Semmel, Glenn S.

    2008-01-01

    The numerous benefits of automatic application code generation are widely accepted within the software engineering community. A few of these benefits include raising the abstraction level of application programming, shorter product development time, lower maintenance costs, and increased code quality and consistency. Surprisingly, code generation concepts have not yet found wide acceptance and use in the field of programmable logic controller (PLC) software development. Software engineers at the NASA Kennedy Space Center (KSC) recognized the need for PLC code generation while developing their new ground checkout and launch processing system. They developed a process and a prototype software tool that automatically translates a high-level representation or specification of safety critical application software into ladder logic that executes on a PLC. This process and tool are expected to increase the reliability of the PLC code over that which is written manually, and may even lower life-cycle costs and shorten the development schedule of the new control system at KSC. This paper examines the problem domain and discusses the process and software tool that were prototyped by the KSC software engineers.

  1. Homeplug AV and IEEE 1901 a handbook for PLC designers and users

    CERN Document Server

    Latchman, Haniph A; Yonge, Larry; Gavette, Sherman

    2013-01-01

      The only authorized book explaining the HomePlug networking standards HomePlug is a growing technology for creating high-speed Power Line Communication (PLC) networks by transmitting data over in-home or in-office power lines. Users only need to plug adapters into wall outlets to create an instant network of computers, printers, routers, home entertainment

  2. Fuel cycle and waste management: A perspective from British nuclear fuels plc

    International Nuclear Information System (INIS)

    The phrase fuel cycle and waste management implies two separate and distinct activities. British Nuclear Fuels plc (BNFL) has adopted a holistic approach to the fuel cycle that integrates the traditional fuel cycle activities of conversion to uranium hexafluoride, fuel fabrication, power generation, and reprocessing with waste arisings, its subsequent treatment, and disposal

  3. Interdisciplinary Automation and Control in a Programmable Logic Controller (PLC Laboratory

    Directory of Open Access Journals (Sweden)

    Jack Toporovsky, Christine D. Hempowicz & Tarek M. Sobh

    2010-04-01

    Full Text Available The University of Bridgeport’s School of Engineering created a new PLC IndustrialControl Lab to accommodate lab-based, hands-on training using different types ofProgrammable Logic Controllers (PLCs. The Lab and associated courses weredesigned to meet ABET’s EC2000 standards and prepare students for the workforce.

  4. Blind encoding into qudits

    International Nuclear Information System (INIS)

    We consider the problem of encoding classical information into unknown qudit states belonging to any basis, of a maximal set of mutually unbiased bases, by one party and then decoding by another party who has perfect knowledge of the basis. Working with qudits of prime dimensions, we point out a no-go theorem that forbids 'shift' operations on arbitrary unknown states. We then provide the necessary conditions for reliable encoding/decoding

  5. Application of PLC to dynamic control system for liquid He cryogenic pumping facility on JT-60U NBI system

    International Nuclear Information System (INIS)

    The control system of the cryogenic facility in the JT-60 NBI system has been replaced by employing the PLC (Programmable Logic Controller) and SCADA (Supervisory Control And Data Acquisition) system. The original control system was constructed about 20 years ago by specifying the DCS (Distributed Control System) computer to deal with ∼400 feedback loops. Recently, troubles on this control system have increased due to its age-induced deterioration. To maintain the high reliability of the cryogenic facility, a new control system has been planned with the PLC and SCADA systems. Their attractive features include high market availability and cost-effectiveness, however, the use of PLC for such a large facility with ∼400 feedback loops has not been established because of insufficient processing capability of the early PLC. Meanwhile, the recent progress in the PLC enables to use the FBD (function block diagram) programming language for 500 function blocks. By optimizing the function blocks and connecting them in the FBD language, the feedback loops have been successfully replaced from DCS to PLC without a software developer. Moreover, an oscillation of the liquid He level, which often occurs during the cooldown mode of the cryopumps, can be automatically stabilized by easily adding a new process program in the PLC. At present, the new control system has worked well

  6. A Cost Effective Solution for Development Environment for Data Acquisition, Monitoring and Simulation of PLC Controlled Applications

    Directory of Open Access Journals (Sweden)

    O. Bjelica

    2014-06-01

    Full Text Available It is very important to test and monitor the operation of Programmable Logic Controller (PLC in real time (online. Nowadays, conventional, but expensive monitoring systems for PLCs, such as Supervisory Control and Data Acquisition (SCADA systems, software and hardware simulators (or debuggers, are widely used. This paper proposes a user friendly and cost-effective development environment for monitoring, data acquisition and online simulation of applications with PLC. The purpose of this solution is to simulate the process which is controlled by the PLC. The performances of the proposed development environment are presented on the examples of washing machine and dishwasher simulators.

  7. Polarization encoded color camera.

    Science.gov (United States)

    Schonbrun, Ethan; Möller, Guðfríður; Di Caprio, Giuseppe

    2014-03-15

    Digital cameras would be colorblind if they did not have pixelated color filters integrated into their image sensors. Integration of conventional fixed filters, however, comes at the expense of an inability to modify the camera's spectral properties. Instead, we demonstrate a micropolarizer-based camera that can reconfigure its spectral response. Color is encoded into a linear polarization state by a chiral dispersive element and then read out in a single exposure. The polarization encoded color camera is capable of capturing three-color images at wavelengths spanning the visible to the near infrared. PMID:24690806

  8. 76 FR 59013 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2011-09-23

    ... . SUPPLEMENTARY INFORMATION: Airworthiness Directive 2011-08-07, Amendment 39-16657 (76 FR 24798, May 3, 2011...-30-AD; Amendment 39-16657; AD 2011-08-07] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  9. Using PLC for Custom-design of a PID/PWM Program to Control a Heater Temperature

    Directory of Open Access Journals (Sweden)

    Mohammad A.K. Alia

    2007-01-01

    Full Text Available This is a practical approach to control a continuous-time process variable (temperature using a discrete controller (PLC and a custom-designed software control algorithm and hardware interface, in a closed-loop system. Unlike the conventionally known technique of transforming a digital control action into a proportional analogue one by a D/A converter followed by power amplifier, the control action was formed internally by the PLC as a PID/PWM signal, which was used to trigger the power stage. This approach helped reducing the error due D/A conversion, maximized utilization of the PLC and reduced total cost. The control system is realized by utilizing the heater of the training board (RGT1, manufactured by DL-lorenzo Company and it simulates an analogue heating control system. The PLC is Siemens Simatic S7-214 model. An experimental result has shown that the control algorithm functions perfectly.

  10. Negative Beta Encoder

    CERN Document Server

    Kohda, Tohru; Aihara, Kazuyuki

    2008-01-01

    A new class of analog-digital (A/D), digital-analog (D/A) converters as an alternative to conventional ones, called $\\beta$-encoder, has been shown to have exponential accuracy in the bit rates while possessing self-correction property for fluctuations of amplifier factor $\\beta$ and quantizer threshold $\

  11. Plasmids encoding therapeutic agents

    Science.gov (United States)

    Keener, William K.

    2007-08-07

    Plasmids encoding anti-HIV and anti-anthrax therapeutic agents are disclosed. Plasmid pWKK-500 encodes a fusion protein containing DP178 as a targeting moiety, the ricin A chain, an HIV protease cleavable linker, and a truncated ricin B chain. N-terminal extensions of the fusion protein include the maltose binding protein and a Factor Xa protease site. C-terminal extensions include a hydrophobic linker, an L domain motif peptide, a KDEL ER retention signal, another Factor Xa protease site, an out-of-frame buforin II coding sequence, the lacZ.alpha. peptide, and a polyhistidine tag. More than twenty derivatives of plasmid pWKK-500 are described. Plasmids pWKK-700 and pWKK-800 are similar to pWKK-500 wherein the DP178-encoding sequence is substituted by RANTES- and SDF-1-encoding sequences, respectively. Plasmid pWKK-900 is similar to pWKK-500 wherein the HIV protease cleavable linker is substituted by a lethal factor (LF) peptide-cleavable linker.

  12. 76 FR 65997 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engines

    Science.gov (United States)

    2011-10-25

    ...-08, Amendment 39-16707 (76 FR 30529, May 26, 2011), and adding the following new AD: Rolls-Royce plc..., Amendment 39-16707 (76 FR 30529, May 26, 2011). (c) Applicability This AD applies to Rolls-Royce plc RB211... (76 FR 30529, May 26, 2011), for all RR RB211-535E4-37, -535E4-B-37, -535E4-B- 75, and...

  13. Design of a New Virtual Interaction Based PLC Training Using Virtual Sensors and Actuators: System and Its Application

    OpenAIRE

    Yoon Sang Kim; Hak-Man Kim

    2013-01-01

    This paper proposes a new virtual interaction based programmable logic controller (PLC) training system using virtual sensors and actuators. The proposed system is composed of four components including virtual sensors (for interacting between user and input devices), virtual actuators (for manipulating and controlling device or equipment), virtual PLC (for programming and generating command), and virtual networks (for interfacing the interactions and transferring the data between all the comp...

  14. Development of Remote Monitoring and a Control System Based on PLC and WebAccess for Learning Mechatronics

    OpenAIRE

    Wen-Jye Shyr; Te-Jen Su; Chia-Ming Lin

    2013-01-01

    This study develops a novel method for learning mechatronics using remote monitoring and control, based on a programmable logic controller (PLC) and WebAccess. A mechatronics module, a Web‐CAM and a PLC were integrated with WebAccess software to organize a remote laboratory. The proposed system enables users to access the Internet for remote monitoring and control of the mechatronics module via a web browser, thereby enhancing work flexibility by enabling personnel to control mechatronics equ...

  15. Saltwell Leak Detector Station Programmable Logic Controller (PLC) Software Configuration Management Plan (SCMP)

    Energy Technology Data Exchange (ETDEWEB)

    WHITE, K.A.

    2000-11-28

    This document provides the procedures and guidelines necessary for computer software configuration management activities during the operation and maintenance phases of the Saltwell Leak Detector Stations as required by HNF-PRO-309, Rev. 1, Computer Software Quality Assurance, Section 2.4, Software Configuration Management. The software configuration management plan (SCMP) integrates technical and administrative controls to establish and maintain technical consistency among requirements, physical configuration, and documentation for the Saltwell Leak Detector Station Programmable Logic Controller (PLC) software during the Hanford application, operations and maintenance. This SCMP establishes the Saltwell Leak Detector Station PLC Software Baseline, status changes to that baseline, and ensures that software meets design and operational requirements and is tested in accordance with their design basis.

  16. Saltwell PIC Skid Programmable Logic Controller (PLC) Software Configuration Management Plan

    Energy Technology Data Exchange (ETDEWEB)

    KOCH, M.R.

    1999-11-16

    This document provides the procedures and guidelines necessary for computer software configuration management activities during the operation and maintenance phases of the Saltwell PIC Skids as required by LMH-PRO-309, Rev. 0, Computer Software Quality Assurance, Section 2.6, Software Configuration Management. The software configuration management plan (SCMP) integrates technical and administrative controls to establish and maintain technical consistency among requirements, physical configuration, and documentation for the Saltwell PIC Skid Programmable Logic Controller (PLC) software during the Hanford application, operations and maintenance. This SCMP establishes the Saltwell PIC Skid PLC Software Baseline, status changes to that baseline, and ensures that software meets design and operational requirements and is tested in accordance with their design basis.

  17. Simulační modely EDUmod pro PLC v NI LabVIEW

    OpenAIRE

    Jančík, Stanislav

    2008-01-01

    Diplomová práce se zabývá propojením programovatelného automatu (PLC) s osobním počítačem (PC) za účelem řízení softwarových simulací v NI LabVIEW. Diplomová práce je rozdělena na dvě hlavní části. Navrhnout a zhotovit komunikační jednotku, která bude mít na starost komunikaci mezi PC a PLC a naprogramovat simulační modely v softwaru NI LabVIEW.

  18. Analysis and Design of PLC-based Control System for Automatic Beverage Filling Machine

    Directory of Open Access Journals (Sweden)

    Yundan Lu

    2015-01-01

    Full Text Available Automatic filling system is the main equipment in the food machinery industry. With the development of beverage industry and increasing demand of the filling system. The relay control method in traditional Filling machine has low automation and integration level and cannot satisfy the rapid development of automatic production. PLC control method has advantages of simple programming, strong anti-interference and high working reliability, has gradually replace the relay control method. In this study, hardware and software for the automatic filling system based on PLC control is designed, especially the injection section servo control system which adopts the servo motor driver metering pump is carefully analyzed and the filling precision is highly improved.

  19. Development of a PLC modem for data transmission over a PWM power supply

    Science.gov (United States)

    Batard, Christophe; Ginot, Nicolas; Mannah, Marc Anthony; Millet, Christophe; Poitiers, Frédéric

    2014-04-01

    In variable-speed electrical drive and online conditioning monitoring, a feedback loop is required in order to transmit the sensor information from the motor to the controller close to the inverter. Additional cabling is used for signalling. This extra cabling has a significant cost and data transmission may not be reliable. Thus, the use of power line communication (PLC) technology to transmit data in motor drive application is quite interesting. The use of a PLC modem dedicated to the home network in a three-phase inverter-fed motor power cable does not work. Therefore, specific coupling interfaces are developed to transmit data through a pulse-width modulated power supply. Laboratory tests have shown that the couplers are operating properly. They ensure reliable data transmission in a motor drive application.

  20. Enhanced Operation of Electricity Distribution Grids Through Smart Metering PLC Network Monitoring, Analysis and Grid Conditioning

    Directory of Open Access Journals (Sweden)

    Iker Urrutia

    2013-01-01

    Full Text Available Low Voltage (LV electricity distribution grid operations can be improved through a combination of new smart metering systems’ capabilities based on real time Power Line Communications (PLC and LV grid topology mapping. This paper presents two novel contributions. The first one is a new methodology developed for smart metering PLC network monitoring and analysis. It can be used to obtain relevant information from the grid, thus adding value to existing smart metering deployments and facilitating utility operational activities. A second contribution describes grid conditioning used to obtain LV feeder and phase identification of all connected smart electric meters. Real time availability of such information may help utilities with grid planning, fault location and a more accurate point of supply management.

  1. PLC based automatic control of pasteurize mix in ice cream production

    Science.gov (United States)

    Yao, Xudong; Liang, Kai

    2013-03-01

    This paper describes the automatic control device of pasteurized mix in the ice cream production process.We design a scheme of control system using FBD program language and develop the programmer in the STEP 7-Micro/WIN software, check for any bugs before downloading into PLC .These developed devices will able to provide flexibility and accuracy to control the step of pasteurized mix. The operator just Input the duration and temperature of pasteurized mix through control panel. All the steps will finish automatically without any intervention in a preprogrammed sequence stored in programmable logic controller (PLC). With the help of this equipment we not only can control the quality of ice cream for various conditions, but also can simplify the production process. This control system is inexpensive and can be widely used in ice cream production industry.

  2. Developing a Novel USB-PLC Controller for a Mechatronics Cloud Laboratory

    Directory of Open Access Journals (Sweden)

    Wen-Jye Shyr

    2013-04-01

    Full Text Available This study proposes the development and implementation of a novel Universal Serial Bus (USB‐Programmable Logic Controller (PLC, called a USB‐PLC controller, for a mechatronics cloud laboratory. The aim of a mechatronics cloud laboratory is to provide state of the art research quality equipment to students, allowing them to conduct hands‐on experiments via the Internet. One objective of the cloud laboratory is to not only provide equipment for conducting set experiments, but also to provide a means for students to access research equipment in order to conduct individual research experiments. The proposed controller for these cloud laboratory experiments has been chosen in order to expose the students to as many different engineering and technology disciplines as possible.

  3. Saltwell PIC Skid Programmable Logic Controller (PLC) Software Configuration Management Plan

    International Nuclear Information System (INIS)

    This document provides the procedures and guidelines necessary for computer software configuration management activities during the operation and maintenance phases of the Saltwell PIC Skids as required by LMH-PRO-309/Rev. 0, Computer Software Quality Assurance, Section 2.6, Software Configuration Management. The software configuration management plan (SCMP) integrates technical and administrative controls to establish and maintain technical consistency among requirements, physical configuration, and documentation for the Saltwell PIC Skid Programmable Logic Controller (PLC) software during the Hanford application, operations and maintenance. This SCMP establishes the Saltwell PIC Skid PLC Software Baseline, status changes to that baseline, and ensures that software meets design and operational requirements and is tested in accordance with their design basis

  4. Analysis and Design of PLC-based Control System for Automatic Beverage Filling Machine

    OpenAIRE

    Yundan Lu; Liangcai Zeng; Feilong Zheng; Gangsheng Kai

    2015-01-01

    Automatic filling system is the main equipment in the food machinery industry. With the development of beverage industry and increasing demand of the filling system. The relay control method in traditional Filling machine has low automation and integration level and cannot satisfy the rapid development of automatic production. PLC control method has advantages of simple programming, strong anti-interference and high working reliability, has gradually replace the relay control method. In this ...

  5. PEMBUATAN SISTEM KONTROL MESIN CAULKING ROD GUIDE OTOMATIS MENGGUNAKAN PLC OMRON CPM1A

    Directory of Open Access Journals (Sweden)

    Syahril Ardi

    2015-02-01

    Full Text Available Kenaikan tingkat produksi shock absorber perusahaan manufaktur membuat proses produksi harus berjalan dengan baik . Pipa proses pemotongan pada mendempul panduan mesin batang memiliki beberapa masalah yang harus diatasi . Untuk mengatasi masalah tersebut kemudian melakukan modifikasi mesin batang mendempul yang secara otomatis dapat mengurangi waktu siklus pada proses mendempul batang dari 4,5 detik per potong sehingga tingkat produksi memotong garis pipa dapat ditingkatkan . Sistem kontrol otomatis mendempul mesin batang panduan ini dirancang untuk menggunakan Programmable Logic Controller (PLC

  6. Finanční analýza Polymetal International Plc

    OpenAIRE

    Cristina ENACHE

    2014-01-01

    The objective of this Master's thesis is to evaluate the financial situation of Polymetal International Plc for the period between 2008 - 2012 using standard financial analysis techniques and procedures, outline the foreign accounting reporting standards and indicate the relation between company's financial results and market gold price change. The thesis consists of two main parts: theoretical and practical. In the theoretical part are defined the main financial analysis techniques and are d...

  7. A PLC DATABASE – A SELECTION ENGINE FOR EFFECTIVE DEPLOYMENT OF PLC’S

    Directory of Open Access Journals (Sweden)

    LUCIEN NGALAMOU,

    2010-10-01

    Full Text Available This paper presents the design considerations of a programmable logic controller (PLC Database, which can be used for effective selection of PLCs and embedded microcomputers for process control and automation. It is a component of a software tool called Industrial Process Control Studio (IPCS dedicated to industrial process control and automation. IPCS is organized in four major modules according to the criteria of modeling, supervision, humanmachine interfacing, and virtual representation.

  8. Perceived environmental uncertainty : understanding the implications for strategy development processes across Barclays Plc

    OpenAIRE

    Ryder, Jansen

    2005-01-01

    The managerial issue being addressed in this research is perceived environmental uncertainty (experienced by corporate strategists) and its implications for strategy development processes at the strategic business unit level (or business-level strategy) across Barclays Bank PLC. The objectives of the research are achieved through: an extensive review of the strategy and uncertainty literature (Project 1); a series of semi-structured interviews with fifteen members of the Group ...

  9. Drosophila fatty acid taste signals through the PLC pathway in sugar-sensing neurons.

    Directory of Open Access Journals (Sweden)

    Pavel Masek

    Full Text Available Taste is the primary sensory system for detecting food quality and palatability. Drosophila detects five distinct taste modalities that include sweet, bitter, salt, water, and the taste of carbonation. Of these, sweet-sensing neurons appear to have utility for the detection of nutritionally rich food while bitter-sensing neurons signal toxicity and confer repulsion. Growing evidence in mammals suggests that taste for fatty acids (FAs signals the presence of dietary lipids and promotes feeding. While flies appear to be attracted to fatty acids, the neural basis for fatty acid detection and attraction are unclear. Here, we demonstrate that a range of FAs are detected by the fly gustatory system and elicit a robust feeding response. Flies lacking olfactory organs respond robustly to FAs, confirming that FA attraction is mediated through the gustatory system. Furthermore, flies detect FAs independent of pH, suggesting the molecular basis for FA taste is not due to acidity. We show that low and medium concentrations of FAs serve as an appetitive signal and they are detected exclusively through the same subset of neurons that sense appetitive sweet substances, including most sugars. In mammals, taste perception of sweet and bitter substances is dependent on phospholipase C (PLC signaling in specialized taste buds. We find that flies mutant for norpA, a Drosophila ortholog of PLC, fail to respond to FAs. Intriguingly, norpA mutants respond normally to other tastants, including sucrose and yeast. The defect of norpA mutants can be rescued by selectively restoring norpA expression in sweet-sensing neurons, corroborating that FAs signal through sweet-sensing neurons, and suggesting PLC signaling in the gustatory system is specifically involved in FA taste. Taken together, these findings reveal that PLC function in Drosophila sweet-sensing neurons is a conserved molecular signaling pathway that confers attraction to fatty acids.

  10. The Detailed Forms of the LMC Cepheid PL and PLC Relations

    OpenAIRE

    Koen, C.; Kanbur, S.; Ngeow, C.

    2007-01-01

    Possible deviations from linearity of the LMC Cepheid PL and PLC relations are investigated. Two datasets are studied, respectively from the OGLE and MACHO projects. A nonparametric test, based on linear regression residuals, suggests that neither PL relation is linear. If colour dependence is allowed for then the MACHO PL relation is found to deviate more significantly from the linear, while the OGLE PL relation is consistent with linearity. These finding are confirmed by fitting "Generalise...

  11. Radioisotopes in the service of mankind: the work of Amersham International plc

    International Nuclear Information System (INIS)

    The paper concerns Amersham International plc - a high technology company providing specialised radioactive products for use in health care, life sciences research, and industry. A brief description is given of the development of the company, and the rise in the use of radioisotopes in our present day world. Details of Amersham's medical, industrial and research products are given, along with the marketing facilities of the products. (U.K.)

  12. Design and Development of an Automated Multi Axis Solar Tracker Using PLC

    OpenAIRE

    Santhosh Krishna Venkata; J S Rajshekar

    2013-01-01

    Design of a two dimensional automated solar tracking system is discussed in this paper. The objectives of the proposed work are to design an automated tracking technique using Light Dependent Resistance (LDR), and solar panel power output to position the solar panel to absorb maximum energy. For positioning the solar panel two stepper motors are used, each for positioning in a plane. These stepper motors were driven by a Programmable Logic Controller (PLC).  The controller is designed using w...

  13. An efficient bit-loading algorithm with peak BER constraint for the band-extended PLC

    OpenAIRE

    Maiga, Ali; Baudais, Jean-Yves; Hélard, Jean-François

    2009-01-01

    ISBN: 978-1-4244-2936-3 International audience Powerline communications (PLC) have become a viable local area network (LAN) solution for in-home networks. In order to achieve high bit rate over powerline, the current technology bandwidth is increased up to 100 MHz within the European project OMEGA. In this paper, an efficient bit-loading algorithm with peak BER constraint is proposed. This algorithm tries to maximize the overall data rate based on linear precoded discrete multitone (LP-...

  14. Research on Automatic Irrigation Algorithm of Strawberry Greenhouse Based on PLC

    OpenAIRE

    Jianlun, Wang; Shuting, Wang; Jianshu, Chen; Hao, Liu; Dongbo, Xu

    2013-01-01

    International audience In order to realize efficient water-saving irrigation of strawberry greenhouse in precision agriculture, the paper proposed an automatic irrigation algorithm based on fuzzy comprehensive evaluation irrigation based on PLC and the configuration software, and build a remote control system. By measuring the greenhouse soil parameters and strawberry soil moisture data to determine the upper and lower limits of irrigation and set buried position of moisture sensors. Use f...

  15. Analysing the importance of internal auditors for John Holt Nigeria Plc.

    OpenAIRE

    Tijani, Gbenga

    2014-01-01

    This bachelor’s thesis deals with analyzing the importance of internal auditors for the company. It aims to solve the issue about the way the new management of John Holt Nigeria Plc perceives the internal audit department. The management of the company believe that the organization can function effectively without the internal audit department. Therefore, as a result of the ongoing downsizing process in the company, the management is considering scrapping the audit department. The main object...

  16. What do innovators do to succeed? A case study of Sage plc

    OpenAIRE

    Oliveira, Manuel Au-Yong; Barandas, Hortênsia; Barros, António

    2007-01-01

    What do innovators do in their efforts to succeed? Successful innovation is embedded and leveraged by the management strategy. Sage plc, the third largest management software company in the world, is analysed. Various factors are seen to foster an innovation culture at Sage: an open organizational culture, a responsive market orientation coupled with a proactive market orientation, an international expansion strategy according to a multi-domestic orientation, and a horizontal i...

  17. Generation of Electricity by Renewable Energy Sources & Transmission of Energy Production Units using PLC & SCADA

    OpenAIRE

    Rahul N Deshmukh ,P.H. Zope ,S.R. Suralkar

    2012-01-01

    ABSTRACTAutomationmeans Delegation of human controlto machine. A PLC (Programmable LogicController) is a device that was invented toreplace the necessary sequential relay circuits formachine control. A SCADA (Supervisory Control& Data Acquisition System) is used to control theprocess where person cannot go or stay for longerperiod. The aim of tis paper is to provideinformation about hoelectricity can be generatedfrom Renewable sources & how its transmissiondone usin automation system. Renewab...

  18. Information system for analysis and performance management of the power plants of CEZ, Plc

    International Nuclear Information System (INIS)

    From 2001 CEZ, Plc provides all power plants units equal and superior to 100 MW with PTIS Information System. PTIS analyses operation of technology equipment and provides calculations in almost real time. To the date of the conference Infoenergo 2004 the power plants Tusimice, Pocerady, Tisova, Prunerov, Melnik and Detmarovice have been equipped by this system called PTIS. The supplier of the PTIS System is OSC Plc, Brno which altogether with sub-supplier ORGREZ Plc, Brno implemented PTIS to the above-mentioned CEZ organizational units. PTIS works with the data obtained from the system of control and power plant management. A part of PTIS's implementation is a huge quality survey of data including control of selected measuring strings. Data are obviously processed to the PTIS System in the strings every 10 seconds. Typical range of applying measuring points for 200 MW unit are 800 by analogy values and 200 binary values. Data from out of unit power plant operations have got similar range. (authors)

  19. Application of MicroLogixTM 1200 PLC in Battery OCV Test

    Institute of Scientific and Technical Information of China (English)

    Zhang Zhiyuan; Yang Yonggang; Zhao Xiaolong; Wu Fengming

    2004-01-01

    The application of MicroLogixTM 1200 PLC made by American AB Company in battery automatic production line located in Inner Mongolia Rare Earth Ovonic High-Power MH/Ni Battery Co., Ltd was introduced.OCV namely open circuit voltage could be somewhat different because of the build materials of negative electrode, positive electrode and electrolyte.So OCV test can check the voltage value of electrolyte filled cells and figure out if the value is within the process specification.MicroLogixTM 1200 relay output type PLC, DeviceNet communication card, variable speed driver, Panelview 1000 color touch screen and some other hardwares were chosen.Then programme by using RSLOGIX500 software, a flexible and skillful use of shift and XOR command in PLC system can make the equipments realize the high automation.Through DeviceNet network based on CAN and with the advantages of quick response and high reliability transmit the information to supervise and control master panel, the system with these components can successfully realize proceeding the battery OCV test and control.The equipments are running stably, locating and test accuracy satisfy the process request.

  20. Encoded combinatorial chemistry.

    OpenAIRE

    Brenner, S; Lerner, R A

    1992-01-01

    The diversity of chemical synthesis and the power of genetics are linked to provide a powerful, versatile method for drug screening. A process of alternating parallel combinatorial synthesis is used to encode individual members of a large library of chemicals with unique nucleotide sequences. After the chemical entity is bound to a target, the genetic tag can be amplified by replication and utilized for enrichment of the bound molecules by serial hybridization to a subset of the library. The ...

  1. PLC Input and Output Points of the Expansion Method%PLC输入输出点数的扩展方法

    Institute of Scientific and Technical Information of China (English)

    刘冬梅

    2011-01-01

    In designing a PLC control system,PLC may encounter the problem of inadequate input and output points,without increasing the PLC configuration in the case,in addition to the use of external resources through the expansion of hardware point PLC,the PLC can also use the internal resources by programming the software for PLC extension points.%在设计一个PLC控制系统时,可能会遇到PLC输入输出点数不足的问题,在不增加PLC配置的情况下,除利用外部资源通过硬件实现PLC点数的扩展,还可利用PLC的内部资源通过编程即软件来实现PLC点数的扩展。

  2. Prospective radiological dose assessment. Amersham plc (Amersham site) variation application December 1998

    International Nuclear Information System (INIS)

    Amersham plc (previously Nycomed-Amersham plc) submitted an application to the Environment Agency in December 1998 for a variation to their radioactive waste discharge authorisations granted under the Radioactive Substances Act 1993. The application requested a reduction in the discharge limits for certain radionuclides and no change for the remaining radionuclides. Amersham plc undertook a further review of their discharge requirements and submitted a new assessment for revised limits in January 2001. This report provides an assessment of the radiological implications of discharges at these revised limits requested by Amersham plc and the limits proposed by the Agency. It has been prepared by the National Compliance Assessment Service at the request of Thames Region to support their determination of the application. Four candidate critical groups were identified who could be exposed to discharges from the Amersham site: 1) Sewage workers at the Maple Lodge sewage works who might be exposed to external radiation from discharges contained within sewage and inadvertently inhale or ingest sewage. 2) Anglers on the Grand Union Canal who eat a small proportion of their annual catch of freshwater fish, who drink water abstracted solely from the River Colne and eat vegetables irrigated by water from the canal. 3) Persons living closest to site who eat locally produced food. 4) Dog walkers living near to site who eat locally produced food. For continuous discharges at the Agency's proposed annual limits, the highest dose of 160 μSv/y is predicted to be received by infants who live closest to the site and eat locally produced food. Therefore, this has been identified as the critical group. Children and adults living at the same location and eating locally produced food receive doses of 140 μSv/y and 130 μSv/y respectively. The critical group dose is less than the source constraint of 300 μSv/y. The dose is dominated by direct radiation from the site (110 μSv/y) and the

  3. Effect of phosphatidylcholine on the level expression of plc genes of Aspergillus fumigatus by real time PCR method and investigation of these genes using bioinformatics analysis.

    Directory of Open Access Journals (Sweden)

    Ali Dehghan-Noodeh

    2014-04-01

    Full Text Available Phosphlipases are a group of enzymes that breakdown phosphatidylcholine (phospholipids molecules producing second products. These produced products have a divers role in the cell like signal transduction and digestion in humans. In this research the effect of phosphatidylcholine on the expression of plc genes of A. fumigatus was studied. The plc genes of this fungus were also interrogated using bioinformatics studies.Real-time PCR was performed to study the expression of plc genes and these genes were interrogated using bioinformatics studies.There was more significant expression for all three plc genes when A. fumigatus was grown on the presence of phosphatidylcholine in the medium. The sequence of plc genes of A. fumigatus was also interrogated using bioinformatics analysis and their relationship with the other microorganisms was investigated.Real-time PCR revealed that afplc1, afplc2 and afplc3 were up-regulated in the presence of phosphatidylcholine. In this study we suggest either the plc's of A. fumigatus were present in an ancestral genome and have become lost in some lineages, or that they have been acquired from other organisms by horizontal gene transfer. We also found that plc's of this fungus appeared to be more closely related to the plant plc's than the bacterial plc's.

  4. Parallel encoders for pixel detectors

    International Nuclear Information System (INIS)

    A new method of fast encoding and determining the multiplicity and coordinates of fired pixels is described. A specific example construction of parallel encodes and MCC for n=49 and t=2 is given. 16 refs.; 6 figs.; 2 tabs

  5. High-speed narrowband PLC - High-performance Access Powerline Communication structures in smart grid; High-Speed Narrowband PLC. Leistungsfaehige Access-Powerline-Kommunikationsstrukturen im Smart Grid

    Energy Technology Data Exchange (ETDEWEB)

    Offner, Georg [devolo AG, Aachen (Germany)

    2012-07-01

    The smart grid provides a stable operation of a decentralized power system which is fed more and more by small providers by means of solar technology and wind power technology. Furthermore, commercial and private clients profit from the smart grid, as they may capture information about their current consumption in real time or can obtain these data from the Internet. The author of the contribution under consideration presents new approaches to access powerline communications that enable efficient communication between IPv6 based household meter and network station. The contribution under consideration describes the technologies used as well as practical experiences and initial results from field tests. Here insights from the integration of G3-PLC are in the foreground.

  6. Data communication between Panasonic PLC and PC using SerialPort control in C#.NET environment

    Science.gov (United States)

    Gao, Ting; Gan, Xiaochuan; Ma, Liqun

    2015-02-01

    With the gradual promotion of Microsoft.NET platform, C# as an object-oriented programming language based on the platform has been widely used. Therefore, more attention is concentrated on how to achieve the communication between Panasonic PLC and PC efficiently and fast in C#.NET environment. In this paper, a method of using SerialPort control which could be used for achieving communication between PLC and PC is introduced. Meanwhile, the reason of abnormal thread when displayed the receiving data in form is analyzed and the programming method to solve the problem of thread safety is designed. Achieving the communication of Panasonic PLC and PC in C#.NET environment can give full play to the advantages of the .NET framework. It is practical, easy communication, high reliability and can combine with other measurement and calibration procedures effectively and conveniently. Configuration software is expensive and can only communicate with PLC separately, but these shortcomings can be solved in C#.NET environment. A well-designed user interface realized real-time monitoring of PLC parameters and achieved management and control integration. The experiment show that this method of data transfer is accurate and the program' running is stable.

  7. Návrh sítě PLC pro dálkový sběr dat

    OpenAIRE

    Šebela, Miroslav

    2008-01-01

    V práci je dle zadání proveden návrh sítě PLC (power line carrier communication) pro dálkový sběr dat. Pro bližší seznámení se s touto technologií je rozvedena problematika komunikace po silovém vedení a možnosti využití PLC technologie. Jsou naznačeny možnosti širokopásmového, zejména však úzkopásmového přenosu dat pomocí PLC. Je popsána obecná struktura PLC sítě pro sběr dat z měřících přístrojů a nakonec je zde popsán návrh PLC sítě na vybrané lokalitě spolu s výběrem dodavatele komponent....

  8. 自动售货机系统的PLC控制设计%PLC Control System Design of Vending Machine

    Institute of Scientific and Technical Information of China (English)

    徐圣龙

    2014-01-01

    Taking Vending machine based on PLC control as an example , this paper introduces the structure of the vending machine , operation process , working principle and function . Automatically trading process is divided into several program modules by PLC programming control , and practice has proved that vending machine with PLC control can improve the stability of the system , ensure the long-term stable operation .%本文以 PLC控制自动售货机,介绍了自动售货机的结构、运行流程、工作原理及功能,将自动交易过程划分为若干个程序模块通过 PLC 编程控制,实践证明,在自动售货机中加入 PLC 的控制可以提高系统的稳定性,保证其长期稳定运行。

  9. PLC的编程语言与编程注意事项%PLC Programming Language and Programming Announcements

    Institute of Scientific and Technical Information of China (English)

    濮海坤

    2013-01-01

    This paper briefly described the basic characteristics of programmable controller (PLC) and development situation, introduces the programming language and its function characteristics of PLC sequence function diagram, ladder diagram, functional block diagram and instruction list, expounds the programming rules and matters needing attention of PLC ladder diagram and instruction list in programming language, etc. On top of the programming language, programming method and the matters needing attention of PLC program has great significance for quick starting to apply PLC programming.%本文简述了可编程控制器(PLC)的基本特点和发展情况,介绍了PLC的顺序功能图、梯形图、功能模块图、指令表和结构文本等编程语言及其功能特点,阐述了PLC在编程过程中梯形图与指令表语言编程的规则和注意事项等。熟练掌握PLC程序的编程语言、编程方法及编程注意事项,对于PLC编程的快速入门及工程应用具有十分重要的意义。

  10. Generating Safety-Critical PLC Code From a High-Level Application Software Specification

    Science.gov (United States)

    2008-01-01

    The benefits of automatic-application code generation are widely accepted within the software engineering community. These benefits include raised abstraction level of application programming, shorter product development time, lower maintenance costs, and increased code quality and consistency. Surprisingly, code generation concepts have not yet found wide acceptance and use in the field of programmable logic controller (PLC) software development. Software engineers at Kennedy Space Center recognized the need for PLC code generation while developing the new ground checkout and launch processing system, called the Launch Control System (LCS). Engineers developed a process and a prototype software tool that automatically translates a high-level representation or specification of application software into ladder logic that executes on a PLC. All the computer hardware in the LCS is planned to be commercial off the shelf (COTS), including industrial controllers or PLCs that are connected to the sensors and end items out in the field. Most of the software in LCS is also planned to be COTS, with only small adapter software modules that must be developed in order to interface between the various COTS software products. A domain-specific language (DSL) is a programming language designed to perform tasks and to solve problems in a particular domain, such as ground processing of launch vehicles. The LCS engineers created a DSL for developing test sequences of ground checkout and launch operations of future launch vehicle and spacecraft elements, and they are developing a tabular specification format that uses the DSL keywords and functions familiar to the ground and flight system users. The tabular specification format, or tabular spec, allows most ground and flight system users to document how the application software is intended to function and requires little or no software programming knowledge or experience. A small sample from a prototype tabular spec application is

  11. Construction of a multi-vender fieldbus system for PC-based control using soft PLC

    Energy Technology Data Exchange (ETDEWEB)

    Park, J. H.; Park, Y. J.; Choi, J. M.; Park, J. S. [Hankyong National Univ., Ansung (Korea)

    2000-12-01

    The relationship between the IEC 61131-3 programming language and the fieldbus application that became necessary as an outcome of the recent automation technology was described.An educational text for the field users on the integration of the Input/Output (I/O) modules and on the IEC 61131-3 programming language was prepared. An attempt was made to include description of the various kind of the fieldbuses and promote understanding of the CAN and the AS-I bus using a practical approach. The profibus is most widely used in the world, and thus is an important and a standardized tool in the area of the process control. A method of integrating the profibus with the most commonly used I/O modules in the field was suggested. The most essential characteristics of the IEC 61131-3 programming language was also described. A method for the construction of the multi-vender I/O modules by the Soft PLC 4C of the softings was suggested. Various technical steps are necessary for the integration of the I/O modules by the soft PLC. An additional software is needed for the more intelligent devises possessing complex technical functions, such as the control valve, the AC driver among others. The 4C control by the softing which is an important industrial solution for the soft PLC was specifically designed for the profibus. So, an OPC server is needed to exchange between the other fieldbuses while the optimum performance is only achieved by using the profibus card by softing. This implies that the business aspect has played a major role for developing the profibus by softing and the domestic users need to pay careful attention to this point additional software tools, such as the PDM or the comuwinn II, are necessary in the process control area to operate a complex and instruments using the DP/PA segment coupler. 13 refs., 46 figs., 12 tabs. (Author)

  12. FINDING THE OPTIMAL LENGTH OF DATA FRAME IN PLC COMPUTER NETWORK

    Institute of Scientific and Technical Information of China (English)

    Huang Ronghui; Zhou Mingtian; She Kun

    2003-01-01

    The length of data frame is an important parameter in Medium Access Control(MAC) layer protocol. A method of finding the optimal length of data frame when impulsive disturbance is involved in PowerLine Communications (PLC) is proposed. Having analyzed the characteristics of impulsive disturbance in powerline and the collision mechanism between the disturbance and data frame, this letter gives the function of the network channel utilization with the length of data frame in MAC layer and the impulsive disturbance. Our stochastic process simulation shows that it is feasible to get the optimal length of data frame.

  13. FINDING THE OPTIMAL LENGTH OF DATA FRAME IN PLC COMPUTER NETWORK

    Institute of Scientific and Technical Information of China (English)

    HuangRonghui; Zhoumingtian

    2003-01-01

    The length of data frame is an important parameter in Medium Access Control (MAC) layer protocol.A method of finding the optimal length of data frame when impulsive disturdance is inuolved in PowerLine Communications (PLC) is proposed.Having analyzed the characteristics of impulsive disturbance in powerline and the collision mechanism between the disturbance and data frame,this letter gives the function of the network channel utilization with the length of data frame in MAC layer and the impulsive disurbance.Our stochastic process simulation shows that it is feasible to get the optimal length of data frame.

  14. Realization of Intelligent Measurement and Control System for Limb Rehabilitation Based on PLC and Touch Screen

    Science.gov (United States)

    Liu, Xiangquan

    According to the treatment needs of patients with limb movement disorder, on the basis of the limb rehabilitative training prototype, function of measure and control system are analyzed, design of system hardware and software is completed. The touch screen which is adopt as host computer and man-machine interaction window is responsible for sending commands and training information display; The PLC which is adopt as slave computer is responsible for receiving control command from touch screen, collecting the sensor data, regulating torque and speed of motor by analog output according to the different training mode, realizing ultimately active and passive training for limb rehabilitation therapy.

  15. STS-65 PLC Hieb at mockup side hatch prepares to egress via an inflated slide

    Science.gov (United States)

    1994-01-01

    STS-65 Mission Specialist and Payload Commander (PLC) Richard J. Hieb, wearing launch and entry suit (LES) and launch and entry helmet (LEH), sits at the top of the inflated slide at the crew compartment trainer (CCT) side hatch and listens to a crew training staffer's instructions. Hieb practiced post landing emergency escape procedures along with his six STS-65 crewmates. The CCT is located in the Johnson Space Center's (JSC's) Mockup and Integration Laboratory (MAIL) Bldg 9NE. Hieb will join five NASA astronauts and a Japanese payload specialist for the International Microgravity Laboratory 2 (IML-2) mission aboard the Space Shuttle Columbia, Orbiter Vehicle (OV) 102, later this year.

  16. Implementation of RS-485 Communication between PLC and PC of Distributed Control System Based on VB

    Science.gov (United States)

    Lian Zhang, Chuan; Da Huang, Zhi; Qing Zhou, Gui; Chong, Kil To

    2015-05-01

    This paper focuses on achieving RS-485 communication between programmable logical controller (PLC) and PC based on visual basic 6.0 (VB6.0) on an experimental automatic production line. Mitsubishi FX2N PLCs and a PC are chosen as slave stations and main station, respectively. Monitoring software is developed using VB6.0 for data input/output, flow control and online parameters setting. As a result, all functions are fulfilled with robust performance. It is concluded from results that one PC can monitor several PLCs using RS-485 communication.

  17. Development of PLC based chiller control system for 3 MeV DC electron beam accelerator

    International Nuclear Information System (INIS)

    A 3 MeV DC electron beam accelerator is under development at Electron beam center, Kharghar, Navi Mumbai. The accelerator has various sections, where heat is generated during operation due to factors contributed by efficiency. The areas that produce heat such as beam dump, HV oscillator, scan horn region etc need to be cooled. A 5TR chiller unit has been installed in a room in the vicinity of the accelerator. Standard chiller unit available in the market do not provide a remote control interface, instead they provide on panel local controllers for the operation of the system. A PLC based interlock has been developed to control all the chiller components such as Cooling tower fan, cooling tower pump, ON/OFF operation of compressor etc. All the components used in chiller unit are interfaced to PLC besides temperature sensor. All machine safety interlock have been introduced by using suitable hardware such as antifreeze coil used to trip compressor in case of control failure may make the ice of water being cooled. The operating point of the chiller has been set to provide water 22-24 degree C. The PLC has been programmed using ladder logic programming method. The system is fully automatic. The system can be operated by setting the set temperature say 22 degree C and temperature hysteresis say 2 degree C. Pressing start button operates different units of the system automatically. Its starts the compressor when the water temperature is 22+2 degree C and stops the compressor when temp reaches at 22 degree C. Any unexpected failure such as 3Phase sequence change/phase failure, over load relay trip, excess temp are indicated by a hooter sound along with fault display on the HMI. A touch screen panel has been provided for human machine interface. This development of control system for chiller sub-system of accelerator has helped us in bringing the chiller unit control from the control room using Ethernet link using Modbus TCP-IP. The implementation of PLC controlled chiller

  18. Boiler level control system based on ControlLogix5550 PLC

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    <正>This paper is a research design based on EFPT process control device.In the design,actual industry field has been simulated and corresponding modelling has been carried on for the boiler level system.Then the appropriate PID parameter has been sorted out and ControlLogix5550 PLC has been used to control the entire boiler level system. At last,a corresponding control interface has been established and the boiler level has been under a safe and accurate control.

  19. A Numerical Tele-service System and its Realization on a PC-PLC Network

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Modern telecommunication methods have many impacts on manufacturing, and tele-service has become one of the strategic approaches to realize the ideas of a flexible manufacturing system. Monitoring and maintenance of production lines from the remote monitor center is an important application of such techniques. This paper presents a typical model of tele-service systems and discusses the underlying challenges. RMMST (remote monitoring and maintenance system testbed), based on PC-PLC and their networks which are used widely in production lines, is presented in detail as a concrete example. With the support of digital technique, RMMST can be used in the monitoring and maintenance of remote production lines conveniently.

  20. Software design analysis technique for the development of PLC-based safety-critical systems

    Energy Technology Data Exchange (ETDEWEB)

    Koo, Seo Ryong; Seong, Poong Hyun [Korea Advanced Institute of Science and Technology, Taejeon (Korea, Republic of)

    2005-11-15

    To develop and implement a safety-critical system, the requirements of the system must be analyzed thoroughly during the phases of a software development's life cycle because a single error in the requirements can generate serious software faults. In this study, a nuclear FBD-style design specification and analysis (NuFDS) approach was proposed for PLC based safety-critical systems. The NuFDS approach is suggested in a straightforward manner for the effective and formal specification and analysis of software designs. Accordingly, the proposed NuFDS approach comprises one technique for specifying the software design and another for analyzing the software design.

  1. A Modification of Gamma Surveymeter Dosemeter 3007A for Monitoring Use Ethernet by PLC T100MD Series

    International Nuclear Information System (INIS)

    It has been modified a gamma surveymeter Dosemeter 3007A. The Surveymeter represents analogous surveymeter, so that an interface for data acquisition is required. Acquisition system from surveymeter is added to the voltage amplifier module from 0 - 200 mV to 0 - 5 V. This voltage value will represent of doses 0 - 5 mR/hour. Hereinafter the analogous signal 0 - 5 V as signal of input to peripheral of PLC T100MD series. Data in the form of processed analogous signal presented at local display of PLC. For long distance monitoring, data have been sent to a computer from PLC by ethernet. After this modification, the surveymeter can be used to monitor from long distance. By using Internet Service Provider, monitoring can be done at any time and any where as long as network internet is available. (author)

  2. Fault tolerant PLC system using CPU and I/O redundancy with switch over logic system for nuclear instrumentation

    International Nuclear Information System (INIS)

    Nuclear instrumentation in power plants and fuel reprocessing plants demand very high reliable fault tolerant programmable logic controllers (PLC) since it is directly related to hazardous operation involving safety of plant, operator and in turn public at large. Components of control systems can fail depending on the circumstances and level of preparedness in plants, leading to a minor or major disaster. Utilizing existing technology and configuring system architecture, a fault tolerant PLC can provide superior solution to meet some of the challenges like high reliability, integrity and availability. This paper presents the background, concepts and implementation of fault tolerant PLC architecture using CPU and I/O redundancy with switch over logic system for nuclear instrumentation. (author)

  3. 77 FR 37740 - Stagecoach Group plc and Coach USA, Inc., et al.-Acquisition of Control of Assets-American Coach...

    Science.gov (United States)

    2012-06-22

    ... From the Federal Register Online via the Government Publishing Office DEPARMENT OF TRANSPORATION Surface Transportation Board Stagecoach Group plc and Coach USA, Inc., et al.--Acquisition of Control of..., DOT. ACTION: Notice of Finance Application. SUMMARY: On May 25, 2012, Stagecoach Group plc...

  4. Arabidopsis AtPLC2 Is a Primary Phosphoinositide-Specific Phospholipase C in Phosphoinositide Metabolism and the Endoplasmic Reticulum Stress Response.

    Directory of Open Access Journals (Sweden)

    Kazue Kanehara

    2015-09-01

    Full Text Available Phosphoinositides represent important lipid signals in the plant development and stress response. However, multiple isoforms of the phosphoinositide biosynthetic genes hamper our understanding of the pivotal enzymes in each step of the pathway as well as their roles in plant growth and development. Here, we report that phosphoinositide-specific phospholipase C2 (AtPLC2 is the primary phospholipase in phosphoinositide metabolism and is involved in seedling growth and the endoplasmic reticulum (ER stress responses in Arabidopsis thaliana. Lipidomic profiling of multiple plc mutants showed that the plc2-1 mutant increased levels of its substrates phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate, suggesting that the major phosphoinositide metabolic pathway is impaired. AtPLC2 displayed a distinct tissue expression pattern and localized at the plasma membrane in different cell types, where phosphoinositide signaling occurs. The seedlings of plc2-1 mutant showed growth defect that was complemented by heterologous expression of AtPLC2, suggesting that phosphoinositide-specific phospholipase C activity borne by AtPLC2 is required for seedling growth. Moreover, the plc2-1 mutant showed hypersensitive response to ER stress as evidenced by changes in relevant phenotypes and gene expression profiles. Our results revealed the primary enzyme in phosphoinositide metabolism, its involvement in seedling growth and an emerging link between phosphoinositide and the ER stress response.

  5. The command of the Flour Transport Pumps in a Cereal Mill with PLC Siemens S7-1200

    Directory of Open Access Journals (Sweden)

    Eugen Răduca

    2014-09-01

    Full Text Available This paper presents pump control with PLC Siemens Simatic S7 - 1200, for transporting flour designed to be implemented in the Pfalzmühle Mannheim. The order was designed to replace the old system made in various traditional contacts and relays. Programming PLC Simatic S7 - 1200, which is a powerful and reliable, a new generation, was conducted in FBD. In order to verify the accuracy and to optimize the operation of the control system was built a functional model.

  6. Design and construction of a VHGT-attached WDM-type triplex transceiver module using polymer PLC hybrid integration technology

    Science.gov (United States)

    Jerábek, Vitezslav; Hüttel, Ivan; Prajzler, Václav; Busek, K.; Seliger, P.

    2008-11-01

    We report about design and construction of the bidirectional transceiver TRx module for subscriber part of the passive optical network PON for a fiber to the home FTTH topology. The TRx module consists of a epoxy novolak resin polymer planar lightwave circuit (PLC) hybrid integration technology with volume holographic grating triplex filter VHGT, surface-illuminated photodetectors and spot-size converted Fabry-Pérot laser diode in SMD package. The hybrid PLC has composed from a two parts-polymer optical waveguide including VHGT filter section and a optoelectronic microwave section. The both parts are placed on the composite substrate.

  7. EPICS-based surveillance of cooling water for J-PARC MR-RF using PLC/Linux

    International Nuclear Information System (INIS)

    In J-PARC MR, Magnet and RF used common cooling water. However, the copper ion (Magnet origin) in cooling water had a bad influence on RF apparatus. In order to make the cooling-water lines of both groups independent, a new cooling-water line was prepared for RF. Then, supervising system for the line was developed using PLC/Linux, which is used in MR control system (EPICS). Using PLC/Linux has the merit of building systems quickly and reducing the cost. (author)

  8. Security Analysis of PHP Encoder

    Directory of Open Access Journals (Sweden)

    Chunlong Yao

    2013-10-01

    Full Text Available As an open source server-side scripts language, PHP is used more and more widely by Web developers now. Protecting PHP code from being plagiarized is also a hot research issue especially with the rapid development of dynamic web industry and people’s copyright protection consciousness. Usually the developers use PHP encoders to encrypt the PHP codes before selling them out. There are several different kinds of PHP encoders with different performances. In this paper, we analyze and compare the security level of some well-known encoders. From a fully new aspect, we try to analyze the output of the encoders with the random statistical tests, which is never done before. Also, we demonstrate the soundness of our method. We figure out the test suite which is most suitable for PHP encoders and explain the reasons. Finally, we carry out the experiments and draw a conclusion about the security of the PHP encoders based on our results

  9. Design and construction of automatic operating system of double chamber plasma nitriding device PLC based

    International Nuclear Information System (INIS)

    The automatic operating system of double chamber plasma nitriding device has been done. The system is used for operating double chamber plasma nitriding automatically as according to the standard operating procedure by pressing push button on the human machine interface (HMI). The system consists of hardware and software. The hardware was constructed using main components T100MD1616+ PLC module and supported by temperature signal conditioner module, Wheatstone bridge module, isolated amplifier module and EMS 30A H Bridge motor driver module. A software program that is planted on T100MD1616+ PLC using ladder diagrams and Tbasic program. Test system functions performed by inserting a set values of temperature and pressure by pressing the button on the human machine interface (HMI). The test results show that the temperature control with a set of values 100 °C obtained stable coverage of 98 °C to 102 °C, (Δ ± 2 °C) with a 2% tolerance and the output voltage of the DAC is 2.436 volts to 2.913 volts. The pressure control with a set of values 2.169 x 10-1 mbar obtained stable coverage of 1.995 x 10-1 mbar to 2.205 x 10-1 mbar, (Δ ± 0.105 x 10-1 mbar) with a 5% tol. (author)

  10. Design of a Pneumatic Robotic Arm for Solar Cell Tester System By using PLC controller

    Directory of Open Access Journals (Sweden)

    Yousif I. Al Mashhadany

    2013-01-01

    Full Text Available Solar cell testers sort photovoltaic cells according to their electrical performance, tested under simulated sunlight. A variety of testers exist, but they all face a common challenge of handling cells that are very small and thin, which makes it difficult to transport the cells from the conveyer to the storage box. This paper presents a new design for a handling robot with vacuum end-effectors, which uses a PLC controller to govern the movement of the cells and the testing process. The design applies to solar cell testers for monocrystalline, polycrystalline, cadmium telluride (CdTe, and copper indium diselenide (CIS cells. Each cell is tested for efficiency and categorized accordingly into four groups (A to D. A Virtual Reality (VR model was built to simulate the system, keeping in mind real world constraints. Two photoelectric sensors were used to make detections for both the testing process and the robot movement. The PLC controller guides the trajectory of the robot according to the results of the efficiency testing. It was seen that the system worked very well, with the testing process and the robot movement interacting smoothly. The robot trajectory was seen to be highly accurate, and the pick and place operations were done with great precision.

  11. Motion control solution for new PLC-based standard development platform for VLT instrument control systems

    Science.gov (United States)

    Popovic, D.; Brast, R.; Di Lieto, N.; Kiekebusch, M.; Knudstrup, J.; Lucuix, C.

    2014-07-01

    More than a decade ago, due to obsolescence issues, ESO initiated the design and implementation of a custom-made CANbus based motion controller (CAN-RMC) to provide, together with a tailor-made software library (motor library), the motion control capabilities for the VME platform needed for the second generation VLT/VLTI instruments. The CAN-RMC controller has been successfully used in a number of VLT instruments but it has high production costs compared to the commercial off-the-shelf (COTS) industrial solutions available on the market today. In the scope of the selection of a new PLC-based platform for the VLT instrument control systems, ESO has evaluated motion control solutions from the company Beckhoff. This paper presents the investigation, implementation and testing of the PLC/TwinCAT/EtherCAT motion controllers for DC and stepper motors and their adaptation and integration into the VLT instrumentation framework. It reports functional and performance test results for the most typical use cases of astronomical instruments like initialization sequences, tracking, switch position detections, backslash compensation, brake handling, etc. In addition, it gives an overview of the main features of TwinCAT NC/PTP, PLCopen MC, EtherCAT motion control terminals and the engineering tools like TwinCAT Scope that are integrated into the development environment and simplify software development, testing and commissioning of motorized instrument functions.

  12. Controlling the Filling and Capping Operation of a Bottling Plant using PLC and SCADA

    Directory of Open Access Journals (Sweden)

    Kunal Chakraborty

    2015-03-01

    Full Text Available This paper presents basic stages of operation of a bottling plant, i.e. the filling and capping process. The main aim of our paper is to control the filling and capping section of a bottling plant simultaneously. At first a set of empty bottle is run by using a conveyer towards filling section, after the operation, the filled bottles are sent towards the capping section. After successful capping operation, the sealed bottles terminate towards exit and a new set of empty bottle arrive, in this way the process continues. This paper includes the method using which, a bunch of bottles can be filled and capped at one instant of time. This method has made the operation more flexible and time saving. The filling and capping operations are controlled using Programmable Logic Controllers (PLC, as the PLC’s are very much user-efficient, cost-effective and easy to control. By using PLC automation the whole process is kept under control. SCADA (Supervisory Control and Data Acquisition is used to monitor the process by means of a display system.

  13. Automated Water Supply System and Water Theft Identification Using PLC and SCADA

    Directory of Open Access Journals (Sweden)

    Prof. Anubha Panchal,

    2014-04-01

    Full Text Available In today’s world rapid growing urban residential areas, to avoid scarcity of water problems and requirements of consumers, therefore it is supposed to supply adequate water distribution networks are managed automatically. Along with this another problem in the water supply system is that public is using suction pumps to suck the water directly from the home street pipeline. The best way to improve the automation and monitoring architectures which contain a supervision and control system for the real time installation, programmable logic controllers with basic functions communication systems, standard interfaces or dedicated ones with proximity sensors, electrical drive elements, measuring devices, etc. In this project it is proposed to develop the PLC & SCADA based water monitoring and theft prevention. Control System is further coupled to SCADA unit .This paper focuses particularly to a control system for controlling and monitoring within a Water Distribution System. Process automation system based upon utilization of an industrial PLC and PC systems including all the network components represents the best way to improve the water distribution technological process.

  14. 基于RS485的小型PLC ModBus总线通信与实现%Bus Communication and Achieve of the Small PLC ModBus Based on RS485

    Institute of Scientific and Technical Information of China (English)

    杨帆

    2012-01-01

    ModBus作为一种开放式通信协议,愈来愈多地被小型PLC厂家选用,并逐渐成为产品的标准配置之一。首先介绍了汇川H2U系列PLC的ModBus总线通信技术,然后给出了实现H2UPLC与MD320变频器ModBus总线通信的硬件连接、变频器参数设置以及PLC通信程序。实际应用表明该方案工程实现容易、性价比高。%ModBus is increasing to choose by small PLC manufacturers as an open communication protocol, and becoming to one of the standard configuration of the product. Firstly the paper introduces bus communication technology of inovance H2U series PLC ModBus, and then achieves hardware connection,inverters parameter settings and PLC communication program of the H2U PLC and MD320 inverter ModBus bus communication. Practical application shows that the program works easily and cost -effectivly.

  15. The Application of PLC in the CGJ-100 Milling Cutting Machine Control System%PLC 在 CGJ-100铣切机控制系统中的应用研究

    Institute of Scientific and Technical Information of China (English)

    张永聪; 张晨曦; 王晓奎

    2014-01-01

    A ccording to the w orking process ofthe m illing cutting m achine for ROGERS materials,the Delta PLC as the low erm achine,touch screen as hum an-com puterinterface,a milling cutting machine control system , which controls three kinem atic axes through PLC , such as X、Y and Z axes, autom ation is realized. Practical operation show s that the control system im proves the production efficiency.%根据铣切机对罗杰斯板的加工过程,采用台达 PLC 作为下位机,触摸屏作为人机界面,构成铣切机控制系统,通过 PLC 控制 X 、Y 及 Z 3个运动轴,实现控制自动化。在实际运行中表明,采用该控制系统提高了生产效率。

  16. Peri-encoding predictors of memory encoding and consolidation.

    Science.gov (United States)

    Cohen, Noga; Pell, Liat; Edelson, Micah G; Ben-Yakov, Aya; Pine, Alex; Dudai, Yadin

    2015-03-01

    We review reports of brain activations that occur immediately prior to the onset or following the offset of to-be-remembered information and can predict subsequent mnemonic success. Memory-predictive pre-encoding processes, occurring from fractions of a second to minutes prior to event onset, are mainly associated with activations in the medial temporal lobe (MTL), amygdala and midbrain, and with enhanced theta oscillations. These activations may be considered as the neural correlates of one or more cognitive operations, including contextual processing, attention, and the engagement of distinct computational modes associated with prior encoding or retrieval. Post-encoding activations that correlate with subsequent memory performance are mainly observed in the MTL, sensory cortices and frontal regions. These activations may reflect binding of elements of the encoded information and initiation of memory consolidation. In all, the findings reviewed here illustrate the importance of brain states in the immediate peri-encoding time windows in determining encoding success. Understanding these brain states and their specific effects on memory may lead to optimization of the encoding of desired memories and mitigation of undesired ones. PMID:25446944

  17. 77 FR 1009 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series Turbofan Engines

    Science.gov (United States)

    2012-01-09

    ..., approved for IBR February 9, 2005 (70 FR 680, January 5, 2005). (3) Rolls-Royce plc Service Bulletin No. RB... listed in this AD as of February 9, 2005 (70 FR 680, January 5, 2005). ADDRESSES: For service information... CFR part 39 to supersede airworthiness directive (AD) 2004-26- 05, amendment 39-13917 (70 FR...

  18. 75 FR 49368 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 900 Series Turbofan Engines

    Science.gov (United States)

    2010-08-13

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have...-13-AD; Amendment 39-16384; AD 2010-16-07] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  19. Professional Learning Communities: An Analysis of Teacher Participation in a PLC and the Relationship with Student Academic Achievement

    Science.gov (United States)

    Aylsworth, Anthony James

    2012-01-01

    This study sought to compare teacher participation in a Professional Learning Community with the performance of their students. Student achievement data from multiple subject-alike groups were compared in a pre-and post-PLC format, using an independent, two-sample t-test. Overall, 10 PLCs from one high school in a suburban, Iowa setting were…

  20. 75 FR 34172 - Rexam Closure Systems, Inc., a Subsidiary of Rexam PLC, Including On-Site Leased Workers From...

    Science.gov (United States)

    2010-06-16

    ... published in the Federal Register on April 23, 2010 (75 FR 21357). The notice was amended on May 11, 2010 to... notice was published in the Federal Register on May 21, 2010 (75 FR 28655). At the request of a company... Employment and Training Administration Rexam Closure Systems, Inc., a Subsidiary of Rexam PLC, Including...

  1. MHC class I signaling in T cells leads to tyrosine kinase activity and PLC-gamma 1 phosphorylation

    DEFF Research Database (Denmark)

    Skov, S; Odum, Niels; Claesson, M H

    1995-01-01

    phosphorylation and the subsequent calcium response. The early tyrosine kinase activity was found to be dependent on expression of the TCR/CD3 complex and the CD45 molecule on the surface of the T cells. Furthermore, MHC-I cross-linking was shown to tyrosine phosphorylate PLC-gamma 1 (phospholipase C-gamma 1...

  2. 76 FR 72650 - Airworthiness Directives; Rolls-Royce plc (RR) RB211 Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2011-11-25

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  3. 78 FR 39062 - Group Lotus plc; Modification of a Temporary Exemption From an Advanced Air Bag Requirement of...

    Science.gov (United States)

    2013-06-28

    ... small manufacturers such as Lotus as of September 1, 2012. \\1\\ 78 FR 15114. After publication of Lotus's... exemption, in control of the company's dealers. See 60 FR 12281 (Mar. 6, 1995). The 50 vehicles will count... National Highway Traffic Safety Administration Group Lotus plc; Modification of a Temporary Exemption...

  4. 75 FR 45560 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 800 Series Turbofan Engines

    Science.gov (United States)

    2010-08-03

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  5. 75 FR 61361 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engines

    Science.gov (United States)

    2010-10-05

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  6. 75 FR 69611 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 768, 772, and 772B Turbofan Engines

    Science.gov (United States)

    2010-11-15

    ...-10508 (63 FR 24911, May 6, 1998): Rolls-Royce plc: Docket No. FAA-2010-0960; Directorate Identifier 98... the DOT's complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR...-27 (63 FR 24911, May 6, 1998). On April 30, 2001, the FAA Transport Airplane Directorate...

  7. 76 FR 12277 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 768, 772, and 772B Turbofan Engines

    Science.gov (United States)

    2011-03-07

    ..., Amendment 39-10508 (63 FR 24911, May 6, 1998): 98-09-27R1 Rolls-Royce plc: Amendment 39-16620. Docket No...: ] Discussion On April 23, 1998, the FAA Engine & Propeller Directorate issued engine AD 98-09-27 (63 FR 24911... (66 FR 23838, May 10, 2001). Those ADs both require the same initial and repetitive visual...

  8. 75 FR 15326 - Airworthiness Directives; Rolls-Royce plc RB211-Trent 500, 700, and 800 Series Turbofan Engines

    Science.gov (United States)

    2010-03-29

    ...-16092 (74 FR 62222, November 27, 2009), with a proposed AD. The proposed AD applies to Rolls-Royce plc... regulations as of January 4, 2010 (74 FR 6222, November 27, 2009). ADDRESSES: The Docket Operations office is... Register on January 6, 2010 (75 FR 801). That action proposed to correct an unsafe condition for...

  9. 76 FR 14797 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 900 Series Turbofan Engines

    Science.gov (United States)

    2011-03-18

    ... Register published on April 11, 2000 (65 FR 19477-78). Authority for This Rulemaking Title 49 of the United... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or...-05-AD; Amendment 39-16636; AD 2011-06-11] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  10. 76 FR 30529 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-535 Series Turbofan Engines

    Science.gov (United States)

    2011-05-26

    ... Register on October 5, 2010 (75 FR 61361). That NPRM proposed to correct an unsafe condition for the...'' under the DOT Regulatory Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have...-39-AD; Amendment 39-16707; AD 2011-11-08] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  11. 76 FR 40217 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series Turbofan Engines

    Science.gov (United States)

    2011-07-08

    ... Register published on April 11, 2000 (65 FR 19477-78). Authority for This Rulemaking Title 49 of the United... Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic impact, positive or...; AD 2011-13-01] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series...

  12. Design and application of the high-voltage DC power-supply control system based on PLC

    International Nuclear Information System (INIS)

    The design and application of A kind of high-voltage DC power-supply control system based on PLC is referred, in addition, KingView is used to monitor the system in real time and manage the man-machine conversation ideally

  13. PLC-based control system for 10 MeV linear accelerator (LCS) at EBC Kharghar, BARC

    International Nuclear Information System (INIS)

    Currently the 10 MeV Linac is being used for different research and industrial applications. The control system in operation was developed using CAMAC based Data Acquisition System (DAS) and Hard-wired Interlock System. It is proposed to replace the CAMAC system with a state-of-the-art indigenously developed Programmable Logic Controller (PLC) that is verified to the level of a Class IB computer-based system used in nuclear power plants. A PLC node comprises of two VME bus based CPU boards (PowerPC MPC7447, 600MHz) working in redundant mode. The inputs and outputs are common to both CPUs. The I/O boards are hot swappable and intelligent. An intelligent Ethernet board is used for communication with a PC running the SCADA software and industry standard communication protocols drivers. The PLC hardware and software has undergone rigorous verification and validation. A user-friendly 'Application Development Environment' is provided to the process engineer for building the application using pre-defined function blocks. The LCS developed using PLC is to be used for operating the Linac irradiation facility, remotely as well as locally in a fail-safe mode, with sequential start-up and sequential shut-down. Apart from system status monitoring, data archiving, alarm generation and setpoint adjustments, it monitors the important parameters and trips the Gun Modulator High Voltage (GM HV), the Klystron Modulator High Voltage (KM HV) and the Electron Gun Power Supply (EG PS) on fault conditions. (author)

  14. 75 FR 28655 - Rexam Closure Systems, Inc. a Subsidiary of Rexam PLC Including On-Site Leased Workers From...

    Science.gov (United States)

    2010-05-21

    ... Closure Systems, Inc., a subsidiary of Rexam PLC, Hamlet, North Carolina. The notice was published in the Federal Register on April 23, 2010 (75 FR 21357). At the request of the State Agency, the Department...) Wages Are Paid Through Owens Illinois Manufacturing Hamlet, NC; Amended Certification...

  15. Effects of 5-fluorouracil on biological characteristics and drug resistance mechanisms of liver cancer cell line PLC/RAF/5

    Directory of Open Access Journals (Sweden)

    CHENG Kangwen

    2015-09-01

    Full Text Available ObjectiveTo study the changes in biological characteristics of a liver cancer cell line PLC/RAF/5 after repeated exposure to a chemotherapy drug, 5-fluorouraci (5-FU, and to investigate the relationship between drug-resistant liver cancer cells and liver cancer stem cells. MethodsA low concentration of 5-FU (1 μg/ml was used to treat the human liver cancer cell line PLC/RAF/5 repeatedly to establish the PLC/RAF/5/5-FU cell line. Morphological differences between the two types of cells were observed. The inhibitory effects of different concentrations of 5-FU (0, 0.25, 0.5, 1, 1.5, and 2 μg/ml on the proliferation of the two types of cells were determined using the CCK-8 assay. Apoptosis of the two types of cells after exposure to different concentrations of 5-FU (0.5, 1, and 2 μg/ml for 48 h was analyzed using flow cytometry. The proportions of side population cells in both types of cells were measured using flow cytometry. The colony-forming ability was compared between the two types of cells by the plate colony-forming assay. The expression of Bax, Bcl-2, ABCG2, and FoxM1 proteins in both types of cells was examined by Western blot. Between-group comparison was performed by t test. ResultsThe PLC/RAF/5/5-FU cell line was successfully established using the chemotherapy drug 5-FU. Compared with the PLC/RAF/5 cells, the PLC/RAF/5/5-FU cells had a larger volume, fewer protrusions, a changed shape of a long shuttle, and enhanced refractivity. Moreover, compared with the parent cells, the PLC/RAF/5/5-FU cells had a significantly lower sensitivity to the inhibitory effect of 5-FU on proliferation, a significantly lower proportion of cells at the G0/G1 phase of the cell cycle, significantly higher proportions of cells at the S and G2/M phases, significantly higher resistance to apoptosis, a significantly higher proportion of side population cells, and significantly enhanced proliferation (P<0.05. According to the results of Western blot assay, the

  16. Supervision of the operations including work with radioactive sources in Kozloduy NPP, plc

    International Nuclear Information System (INIS)

    The purpose of this article is to describe the operations with radioactive sources, their movement, transport and the methods of control during the work. Control during their usage is described in company instructions and include all stages of sources life: needs and requires, buying, transport and receive, storage, work, their leave off exploitation and transfer to long term source-storage. Radioactive sources in Kozloduy NPP, Plc are used for metrology assurance of devices for radiation protection measurements and quality control of measurements. That is why we pay attention mainly on this kind of sources. In article is described also requirements to qualification of people who do this work and the radiation protection control during the operations. In article take place operations with sources with short half life, liquid and gaseous, and sources for single use. (author)

  17. Design of Control System for Flexible Packaging Bags Palletizing Production Line Based on PLC

    Science.gov (United States)

    Zheng, Huiping; Chen, Lin; Zhao, Xiaoming; Liu, Zhanyang

    Flexible packaging bags palletizing production line is to put the bags in the required area according to particular order and size, in order to finish handling, storage, loading and unloading, transportation and other logistics work of goods. Flexible packaging bags palletizing line is composed of turning bags mechanism, shaping mechanism, indexing mechanism, marshalling mechanism, pushing bags mechanism, pressing bags mechanism, laminating mechanism, elevator, tray warehouse, tray conveyor and loaded tray conveyor. Whether the whole production line can smoothly run depends on each of the above equipment and precision control among them. In this paper the technological process and the control logic of flexible packaging bags palletizing production line is introduced. Palletizing process of the production line realized automation by means of a control system based on programmable logic controller (PLC). It has the advantages of simple structure, reliable and easy maintenance etc.

  18. A compact 1×64 optical power splitter using silica-based PLC on quartz substrate

    Science.gov (United States)

    Wang, Liangliang; An, Junming; Wu, Yuanda; Wang, Yue; Zhang, Jiashun; Li, Jianguang; Wang, Hongjie; Zhang, Xiaoguang; Pan, Pan; Dai, Hongqing; Qi, Ying; Zhong, Fei; Zha, Qiang; Hu, Xiongwei; Zhao, Degang

    2014-09-01

    In this paper, a compact and low wavelength-dependence loss (WDL) 1×64 optical power splitter is fabricated using silica-based PLC technology on quartz substrate. The cascaded Y-branch structures are optimized in detail, and a compact 1×64 optical power splitter layout is obtained. The measured results show that the insertion loss (IL), the uniformity and WDL of the best results of all splitters are less than 19.2 dB, 1.0 dB and 0.5 dB, respectively, in the wavelength range from 1.26 μm to 1.65 μm, and the total product ratio of Standard grade in a whole 6 in. wafer is more than 80%. The results imply that our technology completely satisfies the need of mass manufacture.

  19. STS-55 MS1/PLC Ross monitors Payload Specialist Walter's Anthrorack activity

    Science.gov (United States)

    1993-01-01

    STS-55 German Payload Specialist 1 Ulrich Walter breathes into Rack 9 Anthrorack (AR) (Human Physiology Laboratory) device for Pulmonary Perfusion and Ventilation During Rest and Exercise experiment while working inside the Spacelab Deutsche 2 (SL-D2) science module aboard the Earth-orbiting Columbia, Orbiter Vehicle (OV) 102. Seated on the bicycle ergometer, Walter utilizes the respiratory monitoring system, part of a broad battery of experiments designed to investigate human physiology under microgravity conditions. In the background, Mission Specialist 1 (MS1) and Payload Commander (PLC) Jerry L. Ross monitors Walter's activity. Walter represents the German Aerospace Research Establishment (DLR) on the 10-day SL-D2 mission. Visible on the aft end cone are a fire extinguisher and the Crew Telesupport Experiment (CTE) Macintosh portable computer mounted on an adjustable work platform.

  20. Advanced design technique of human-machine interfaces for PLC control of complex systems

    Directory of Open Access Journals (Sweden)

    Árpád-István Sütő

    2008-05-01

    Full Text Available Touchscreen operator panels proved to be a convenient succesor for clasical operator panels for implementing human-machine interfaces (HMIs in programmable logic controllers (PLC systems. The paper introduces a new technique for HMIs design in such systems, based on the idea of touchscreens replication. This redundancy allow actions which are not possible within the menus and sub-menus of a single touchscreen. Its strenght is revealed especially in complex systems, where operators can easily be overwhelmed by the huge amount of process information. The technique was applied on a mill tube rolling installation. The results also proved an increase of system security and zero downtime for HMI maintenance activities.

  1. Aspects of environmental monitoring by British Nuclear Fuels plc following the Chernobyl reactor accident

    International Nuclear Information System (INIS)

    The radioactive cloud from the Chernobyl reactor accident arrived in West Cumbria on 2 May 1986. The environmental monitoring facilities of the British Nuclear Fuels plc, Sellafield reprocessing plant were used to monitor radioactivity in air, deposition on grass and on soil and concentrations in milk. The distribution of deposition between sampled grass and soil was affected by heavy rainfall during the passage of the radioactive cloud. Measurements of radioactivity in milk at a lowland farm on the coastal plain resulted in a critical group effective dose of 0.64 mSv up to the end of July, but additional doses are expected to result from the use of silage during the winter. Comparisons are made between these doses from milk consumption and those predicted from the data available shortly after the deposition of the radioactivity on the pasture. (author)

  2. Automatic convey or System with In–Process Sorting Mechanism using PLC and HMI System

    Directory of Open Access Journals (Sweden)

    Y V Aruna

    2015-11-01

    Full Text Available Programmable logic controllers are widely used in many manufacturing process like machinery packaging material handling automatic assembly. These are special type of microprocessor based controller used for any application that needs any kind of electrical controller including lighting controller and HVAC control system. Automatic conveyor system is a computerized control method of controlling and managing the sorting mechanism at the same time maintaining the efficiency of the industry & quality of the products.HMI for automatic conveyor system is considered the primary way of controlling each operation. Text displays are available as well as graphical touch screens. It is used in touch panels and local monitoring of machines. This paper deals with the efficient use of PLC in automatic conveyor system and also building the accuracy in it.

  3. Fault tree synthesis for software design analysis of PLC based safety-critical systems

    Energy Technology Data Exchange (ETDEWEB)

    Koo, S. R.; Cho, C. H. [Corporate R and D Inst., Doosan Heavy Industries and Construction Co., Ltd., 39-3, Seongbok-Dong, Yongin-Si, Gyeonggi-Do 449-795 (Korea, Republic of); Seong, P. H. [Dept. of Nuclear and Quantum Engineering, Korea Advanced Inst. of Science and Technology, 373-3 Guseong-dong, Yuseong-gu, Daejeon, 305-701 (Korea, Republic of)

    2006-07-01

    As a software verification and validation should be performed for the development of PLC based safety-critical systems, a software safety analysis is also considered in line with entire software life cycle. In this paper, we propose a technique of software safety analysis in the design phase. Among various software hazard analysis techniques, fault tree analysis is most widely used for the safety analysis of nuclear power plant systems. Fault tree analysis also has the most intuitive notation and makes both qualitative and quantitative analyses possible. To analyze the design phase more effectively, we propose a technique of fault tree synthesis, along with a universal fault tree template for the architecture modules of nuclear software. Consequently, we can analyze the safety of software on the basis of fault tree synthesis. (authors)

  4. Fault tree synthesis for software design analysis of PLC based safety-critical systems

    International Nuclear Information System (INIS)

    As a software verification and validation should be performed for the development of PLC based safety-critical systems, a software safety analysis is also considered in line with entire software life cycle. In this paper, we propose a technique of software safety analysis in the design phase. Among various software hazard analysis techniques, fault tree analysis is most widely used for the safety analysis of nuclear power plant systems. Fault tree analysis also has the most intuitive notation and makes both qualitative and quantitative analyses possible. To analyze the design phase more effectively, we propose a technique of fault tree synthesis, along with a universal fault tree template for the architecture modules of nuclear software. Consequently, we can analyze the safety of software on the basis of fault tree synthesis. (authors)

  5. Structural basis for the activation mechanism of the PlcR virulence regulator by the quorum-sensing signal peptide PapR

    OpenAIRE

    Grenha, Rosa; Slamti, Leyla; Nicaise, Magali; Refes, Yacine; Lereclus, Didier; Nessler, Sylvie

    2012-01-01

    The quorum-sensing regulator PlcR is the master regulator of most known virulence factors in Bacillus cereus. It is a helix-turn-helix (HTH)-type transcription factor activated upon binding of its cognate signaling peptide PapR on a tetratricopeptide repeat-type regulatory domain. The structural and functional properties of PlcR have defined a new family of sensor regulators, called the RNPP family (for Rap, NprR, PrgX, and PlcR), in Gram-positive bacteria. To fully understand the activation ...

  6. Self-Organising Stochastic Encoders

    CERN Document Server

    Luttrell, Stephen

    2010-01-01

    The processing of mega-dimensional data, such as images, scales linearly with image size only if fixed size processing windows are used. It would be very useful to be able to automate the process of sizing and interconnecting the processing windows. A stochastic encoder that is an extension of the standard Linde-Buzo-Gray vector quantiser, called a stochastic vector quantiser (SVQ), includes this required behaviour amongst its emergent properties, because it automatically splits the input space into statistically independent subspaces, which it then separately encodes. Various optimal SVQs have been obtained, both analytically and numerically. Analytic solutions which demonstrate how the input space is split into independent subspaces may be obtained when an SVQ is used to encode data that lives on a 2-torus (e.g. the superposition of a pair of uncorrelated sinusoids). Many numerical solutions have also been obtained, using both SVQs and chains of linked SVQs: (1) images of multiple independent targets (encod...

  7. Cell encoding recombinant human erythropoietin

    Energy Technology Data Exchange (ETDEWEB)

    Beck, A.K.; Withy, R.M.; Zabrecky, J.R.; Masiello, N.C.

    1990-09-04

    This patent describes a C127 cell transformed with a recombinant DNA vector. It comprises: a DNA sequence encoding human erythropoietin, the transformed cell being capable of producing N-linked and O-linked glycosylated human erythropoietin.

  8. Polymorphisms of mitochondrially encoded proteins.

    OpenAIRE

    Spinner, N B; King, M. C.

    1986-01-01

    Polymorphisms of mitochondrially encoded proteins can be detected in human lymphocytes by sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Using an SDS-polyacrylamide 8 M urea system, 17 mitochondrially encoded proteins are distinguishable. Three of these (ME-6, ME-8, and ME-17) were polymorphic among 92 individuals screened, and these polymorphisms are reported here for the first time. With SDS-polyacrylamide electrophoresis without urea, 18 mitochondrial proteins are de...

  9. Security Analysis of PHP Encoder

    OpenAIRE

    Chunlong Yao; Fengjiao Yin; Xu Li; Fenglong Fan

    2013-01-01

    As an open source server-side scripts language, PHP is used more and more widely by Web developers now. Protecting PHP code from being plagiarized is also a hot research issue especially with the rapid development of dynamic web industry and people’s copyright protection consciousness. Usually the developers use PHP encoders to encrypt the PHP codes before selling them out. There are several different kinds of PHP encoders with different performances. In this paper, we analyze and compa...

  10. Control the Stability and Steady State When the Elevator Reached the Requested Level (PLC (LOGO) Control for4-level elevator system)

    OpenAIRE

    ijera

    2014-01-01

    This paper presenta project on design and implementation of a PLC-based controller for a 4-level elevator Feedback system. The PLC used is a Siemens with 24 inputs and 16 outputs. The design incorporates an intelligent controller that solving the stability and balance, based on algorithm and PID software solution. Some farthersuggestions on how to extend the program for control the system are offered.

  11. Control the Stability and Steady State When the Elevator Reached the Requested Level (PLC (LOGO Control for4-level elevator system

    Directory of Open Access Journals (Sweden)

    ijera

    2014-08-01

    Full Text Available This paper presenta project on design and implementation of a PLC-based controller for a 4-level elevator Feedback system. The PLC used is a Siemens with 24 inputs and 16 outputs. The design incorporates an intelligent controller that solving the stability and balance, based on algorithm and PID software solution. Some farthersuggestions on how to extend the program for control the system are offered.

  12. Data acquisition and control system with a programmable logic controller (PLC) for a pulsed chemical oxygen-iodine laser

    Science.gov (United States)

    Yu, Haijun; Li, Guofu; Duo, Liping; Jin, Yuqi; Wang, Jian; Sang, Fengting; Kang, Yuanfu; Li, Liucheng; Wang, Yuanhu; Tang, Shukai; Yu, Hongliang

    2015-02-01

    A user-friendly data acquisition and control system (DACS) for a pulsed chemical oxygen -iodine laser (PCOIL) has been developed. It is implemented by an industrial control computer,a PLC, and a distributed input/output (I/O) module, as well as the valve and transmitter. The system is capable of handling 200 analogue/digital channels for performing various operations such as on-line acquisition, display, safety measures and control of various valves. These operations are controlled either by control switches configured on a PC while not running or by a pre-determined sequence or timings during the run. The system is capable of real-time acquisition and on-line estimation of important diagnostic parameters for optimization of a PCOIL. The DACS system has been programmed using software programmable logic controller (PLC). Using this DACS, more than 200 runs were given performed successfully.

  13. Improvement of PLC-based data acquisition system for beam loss monitors of the J-PARC main ring

    International Nuclear Information System (INIS)

    A data acquisition system of the beam loss monitor of J-PARC Main Ring consists of the combination of Programmable Logic Controller (PLC). The system needs the high speed response to an external trigger. So we use high speed data acquisition modules to get output data from the beam loss monitor. Triggered by an external trigger pulse, the system begins to acquire the data with some pre-defined intervals by the signal which the system itself generates. We use the new type of a CPU module of PLC, in which an embedded EPICS system on the Linux OS. With the new type of a CPU module, the data acquisition system becomes simpler. This paper reports the state of development and the result in operation. (author)

  14. First Experience with PLC, OPC and BridgeVIEW in the context of the HMPID liquid distribution prototype

    CERN Document Server

    Maatta, E; CERN. Geneva; Swoboda, Detlef; Lecoeur, G

    1999-01-01

    The TESt (Test and Evaluation Station) [3] project consists in the construction of a stand alone unit for a specific sub-system of an ALICE detector in order to gain first experience with commercial products for detector control. Although the control system includes only a small number of devices and is designed for a particular application, it covers nevertheless all layers of a complete system and can be extended or used in different applications. The control system prototype has been implemented for the Perfluorohexane (C6F14) liquid distribution of the ALICE-HMPID (High-Momentum Particle IDentification)[5]. This report presents the experience acquired while developing the control system with off the shelf items: National Instruments BridgeVIEW [6] supervision software, SIEMENS PLC S7-300 [7] and the programming tool STEP 7. The OPC standard (OLE [12] for Process Control) [8] was used for the communication between BridgeVIEW and the PLC.

  15. Implementation of Machinery Failure Mode and Effect Analysis in Amhara Pipe Factory P.L.C., Bahir Dar, Ethiopia

    OpenAIRE

    Yonas Mitiku Degu; R. Srinivasa Moorthy

    2016-01-01

    - Failure Mode and Effect Analysis (FMEA) is a pro-active quality tool for evaluating potential failure modes and their causes. It helps in prioritizing the failure modes and recommends corrective measures for the avoidance of catastrophic failures and improvement of quality. In this work, an attempt has been made to implement Machinery FMEA in UPVC pipe production unit of Amhara Pipe Factory, P.L.C., Bahir Dar, Ethiopia. The failure modes and their causes were identified for each machine, th...

  16. STS-57 MS and PLC G. David Low examines CGBA pack in OV-105's SPACEHAB module

    Science.gov (United States)

    1993-01-01

    STS-57 Mission Specialist (MS) and Payload Commander (PLC) G. David Low examines Commercial Generic Bioprocessing Apparatus (CGBA) group activation pack (GAP) incubator while in front of the SPACEHAB-01 (Commercial Middeck Augmentation Module (CMAM)) aft lockers. Low has temporarily removed the GAP from its locker location. Crewmembers are conducting a variety of experiments inside SPACEHAB-01 module which is positioned in the payload bay (PLB) of the Earth-orbiting Endeavour, Orbiter Vehicle (OV) 105.

  17. The Effectiveness of Human Resource Management Strategies in Organization : A Case Study of United Bank for Africa PLC

    OpenAIRE

    Nnamani, Nnamdi Hezekiah

    2013-01-01

    Having effective human resources management strategies and practically applying it in an organization has a great impact on how competitive they will be in the business environment. The study titled “Effectiveness of Human Resources Management Strategies in the Organization; A case study on United Bank For Africa PLC”, was conducted to determine the success of the human resources management strategies of the UBA PLC and to also find out the relationship between these strategies and the overal...

  18. Regulated release of BDNF by cortical oligodendrocytes is mediated through metabotropic glutamate receptors and the PLC pathway

    Directory of Open Access Journals (Sweden)

    Issa P Bagayogo

    2009-04-01

    Full Text Available A number of studies suggest that OLGs (oligodendrocytes), the myelinating cells of the central nervous system, are also a source of trophic molecules, such as neurotrophins that may influence survival of proximate neurons. What is less clear is how the release of these molecules may be regulated. The present study investigated the effects of BDNF (brain-derived neurotrophic factor) derived from cortical OLGs on proximate neurons, as well as regulatory mechanisms mediating BDNF release. Initial work determined that BDNF derived from cortical OLGs increased the numbers of VGLUT1 (vesicular glutamate transporter 1)-positive glutamatergic cortical neurons. Furthermore, glutamate acting through metabotropic, and not AMPA/kainate or NMDA (N-methyl-d-aspartate), receptors increased BDNF release. The PLC (phospholipase C) pathway is a key mediator of metabotropic actions to release BDNF in astrocytes and neurons. Treatment of OLGs with the PLC activator m-3M3FBS [N-(3-trifluoromethylphenyl)-2,4,6-trimethylbenzenesulfonamide] induced robust release of BDNF. Moreover, release elicited by the metabotropic receptor agonist ACPD [trans-(1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid] was inhibited by the PLC antagonist U73122, the IP3 (inositol triphosphate 3) receptor inhibitor 2-APB (2-aminoethoxydiphenylborane) and the intracellular calcium chelator BAPTA/AM [1,2-bis-(o-aminophenoxy)ethane-N,N,N′,N′-tetra-acetic acid tetrakis(acetoxymethyl ester)]. Taken together, these results suggest that OLG lineage cells release BDNF, a molecule trophic for proximate neurons. BDNF release is regulated by glutamate acting through mGluRs (metabotropic glutamate receptors) and the PLC pathway. Thus glutamate and BDNF may be molecules that support neuron–OLG interactions in the cortex.

  19. PLC Based Adaptive PID Control of Non Linear Liquid Tank System using Online Estimation of Linear Parameters by Difference Equations

    OpenAIRE

    Kesavan.E; Rakesh kumar.S

    2013-01-01

    This paper suggests an idea to design an adaptive PID controller for Non-linear liquid tank System and is implemented in PLC. Online estimation of linear parameters (Time constant and Gain) brings an exact model of the process to take perfect control action. Based on these estimated values, the controller parameters will be well tuned by internal model control. Internal model control is an unremarkably used technique and provides well tuned controller in order to have a good controlling proce...

  20. Finanční analýza společnosti New World Resources Plc

    OpenAIRE

    Valík, Lukáš

    2013-01-01

    This diploma thesis titled "Financial analysis of the company New World Resources Plc" is structured into three main parts. The first chapter lays down the theoretical framework of the financial analysis including the definitions of the fundamental terms, introduces the main groups of users and presents mostly applied tools. The second part aims to present relevant information about the NWR group, i.e. among others the major shareholders and controlled subsidiaries. Moreover, the substantial ...

  1. Gravity referenced elevation encoder development

    Science.gov (United States)

    Goddard, R. E.

    1993-05-01

    Recent progress in the development of a gravity-sensor-based instrument for determining the elevation angle of DSN antennas is described. The benefits of such a system include the capability to locate the Gravity Referenced Elevation Encoder (GREE) directly on the primary reflector (thus bypassing structural flexure and deformation error sources), anticipated lower maintenance costs compared to the present gimbal encoders, direct replaceability, or supplementation of the present gimbal encoders and the utilization of off-the-shelf components to construct the GREE. This article includes a description of the nominal GREE design. Test results on a laboratory breadboard model are given. Rigid-body dynamics of the GREE are derived and the simulated performance in response to measured antenna vibrations is given.

  2. Fly Photoreceptors Encode Phase Congruency

    Science.gov (United States)

    Friederich, Uwe; Billings, Stephen A.; Hardie, Roger C.; Juusola, Mikko; Coca, Daniel

    2016-01-01

    More than five decades ago it was postulated that sensory neurons detect and selectively enhance behaviourally relevant features of natural signals. Although we now know that sensory neurons are tuned to efficiently encode natural stimuli, until now it was not clear what statistical features of the stimuli they encode and how. Here we reverse-engineer the neural code of Drosophila photoreceptors and show for the first time that photoreceptors exploit nonlinear dynamics to selectively enhance and encode phase-related features of temporal stimuli, such as local phase congruency, which are invariant to changes in illumination and contrast. We demonstrate that to mitigate for the inherent sensitivity to noise of the local phase congruency measure, the nonlinear coding mechanisms of the fly photoreceptors are tuned to suppress random phase signals, which explains why photoreceptor responses to naturalistic stimuli are significantly different from their responses to white noise stimuli. PMID:27336733

  3. Inhibition of Emodin on LPS-induced Nitric Oxide Generation by Suppressing PLC-γ Phosphorylation in Rat Peritoneal Macrophages

    Institute of Scientific and Technical Information of China (English)

    WANG Xin-yu; CAI Shou-guang; WU Yi-fen; LI Jun-ying; YANG Wen-xiu; HU Fen

    2010-01-01

    Objective To investigate the inhibitory mechanism of emodin on lipopolysaccharide(LPS)-induced nitric oxide(NO)generation in rat peritoneal macrophages.Methods NO production and iNOS expression were measured through nitrite assay and Western blotting assay,respectively.NF-kB activity and nuclei P65 expression were estimated by dual-luciferase and Western blotting assay,respectively.Intracellular free Ca2+([Ca2+]i)was detected using the ratiometric fluorescent calcium indicator dye,Fura-2,and a microspectrofluorometer.PLC-γphosporylation was analyzed by Western blotting assay.Results First,emodin was found playing active roles in suppressing LPS-induced NF-kB activation in rat peritoneal macrophages.Second,emodin down-regulated transient[Ca2*]i and could increase in NF-kB upstream signal.Finally,emodin suppressed phosphorylation of PLC-γ by LPS stimulation in the upstream of[Ca2+]i.Conclusion Suppression of PLC-γ phosphorylation is involved in emodin inhibiting NO generation by LPS stimulation in rat peritoneal macrophages.

  4. Development of Remote Monitoring and a Control System Based on PLC and WebAccess for Learning Mechatronics

    Directory of Open Access Journals (Sweden)

    Wen-Jye Shyr

    2013-02-01

    Full Text Available This study develops a novel method for learning mechatronics using remote monitoring and control, based on a programmable logic controller (PLC and WebAccess. A mechatronics module, a Web‐CAM and a PLC were integrated with WebAccess software to organize a remote laboratory. The proposed system enables users to access the Internet for remote monitoring and control of the mechatronics module via a web browser, thereby enhancing work flexibility by enabling personnel to control mechatronics equipment from a remote location. Mechatronics control and long‐distance monitoring were realized by establishing communication between the PLC and WebAccess. Analytical results indicate that the proposed system is feasible. The suitability of this system is demonstrated in the department of industrial education and technology at National Changhua University of Education, Taiwan. Preliminary evaluation of the system was encouraging and has shown that it has achieved success in helping students understand concepts and master remote monitoring and control techniques.

  5. Virally encoded 7TM receptors

    DEFF Research Database (Denmark)

    Rosenkilde, M M; Waldhoer, M; Lüttichau, H R; Schwartz, T W

    2001-01-01

    A number of herpes- and poxviruses encode 7TM G-protein coupled receptors most of which clearly are derived from their host chemokine system as well as induce high expression of certain 7TM receptors in the infected cells. The receptors appear to be exploited by the virus for either immune evasion...... expression of this single gene in certain lymphocyte cell lineages leads to the development of lesions which are remarkably similar to Kaposi's sarcoma, a human herpesvirus 8 associated disease. Thus, this and other virally encoded 7TM receptors appear to be attractive future drug targets....

  6. Rockwell Automation PLC-5 Lands Stennis Space Center with a Reliable, Flexible Control System

    Science.gov (United States)

    Epperson, Dave

    2003-01-01

    Ever since the first rocket was launched, people have been infatuated with the vast and unchartered frontier of space. Whether it's visiting a space center or watching a shuttle launch, people are waiting to see what will be discovered next. And even though orbiting the Earth or taking soil samples form the Moon now seems effortless, decades worth of behind-the-scenes work have helped the U.S. space program get to this point. Even today, NASA must take every precaution to ensure equipment is up to the endeavor of setting foot on the moon. As part of the initial push to put the first man on the moon, NASA established the John C. Stennis Space Center, Hancock County, Mississippi in 1961 for space engine propulsion system development. Today, Stennis has three major test complexes where engine and component testing is carried out and integrated into full motion systems for space shuttles and vehicles as well as secondary testing facilities. With different products being tested throughout the facilities, Stennis was in need of an automation system that could link the operations. By integrating a control system based on a Rockwell Automation's flexible and reliable PLC-5 controller, Stennis was able to implement projects more efficiently and focus its efforts on getting the next generation of products ready for space.

  7. Pengaruh Problem-Based Learning terhadap Hasil Belajar ditinjau dari Motivasi Belajar PLC di SMK

    Directory of Open Access Journals (Sweden)

    Bekti Wulandari

    2013-06-01

    Full Text Available Penelitian ini bertujuan untuk mengetahui: (1 perbedaan hasil belajar siswa pada mata pelajaran pemrograman sistem kendali PLC antara siswa yang diajar dengan metode PBL dengan siswa yang diajar dengan metode demonstrasi, (2 pengaruh interaksi antara metode PBL dan metode demonstrasi dengan motivasi belajar terhadap hasil belajar siswa, (3 perbedaan hasil belajar siswa antara siswa yang diajar dengan metode PBL dengan yang diajar dengan metode demonstrasi ditinjau dari motivasi belajar. Penelitian ini merupakan penelitian eksperimen semu dengan desain faktorial yang dilakukan dengan memberikan perlakuan metode pembelajaran. Analisis data dalam penelitian ini  menggunakan uji-t dan ANAVA dengan program SPSS 16. Hasil penelitian menunjukkan bahwa: (1 terdapat perbedaan hasil belajar antara siswa yang diajar dengan metode PBL dengan yang diajar dengan metode demonstrasi, (2 tidak terdapat pengaruh interaksi antara metode PBL dan demonstrasi dengan motivasi belajar terhadap hasil belajar, (3 terdapat perbedaan hasil belajar antara siswa yang diajar dengan metode PBL dengan yang diajar dengan metode demonstrasi ditinjau dari motivasi tinggi dan rendah. Kata kunci: metode pembelajaran, Problem-Based Learning, motivasi belajar, hasil belajar

  8. Department of Energy: monitoring and control of British Nuclear Fuels plc

    International Nuclear Information System (INIS)

    British Nuclear Fuels plc (BNFL) was set up in 1971 to take over the nuclear fuel production and reprocessing activities of the United Kingdom Atomic Energy Authority with the Department of Energy (as majority shareholder) being responsible for the monitoring and control of BNFL's activities. BNFL's activities include the production of nuclear fuel, uranium enrichment, and the transportation and reprocessing of spent fuel. Its major capital investment includes the construction of the Thermal Oxide Reprocessing Plant (THORP) due for completion in 1992. This study examined the effectiveness of the Department's arrangements for monitoring and control and for safeguarding the Government's investment in the company, the arrangements for examining BNFL's capital investment programme and the extent to which the Department's main aims have been achieved. The examination was restricted to the financial performance. The National Audit Office found evidence to suggest that BNFL's financial performance has not kept pace with the general performance level of British Industry. Future success and performance will depend on the success of the THORP plant. (U.K.)

  9. Synthesis of FBD-based PLC design from NuSCR formal specification

    International Nuclear Information System (INIS)

    NuSCR is a formal specification language to document requirements for real-time embedded software with nuclear engineering applications in mind. Domain experts actively participated in selecting how to best represent various aspects. It uses tabular notations to specify required computations and automata to document state- or time-dependent behavior. As programmable logic controllers (PLCs) are widely used to implement real-time embedded software, synthesis of PLC code from a formal specification is desirable if transformation rules can be rigorously defined. In addition to improved productivity, results of safety analysis performed on requirements remain valid. In this paper, we demonstrate how NuSCR specification can be translated into semantically equivalent function block diagram (FBD) code. The process, except the initial phase where user provides information on missing or implicit details, is automated. Since executable code can be automatically generated using CASE tools from FBD, much of software development is automated. Proposed technique is currently being used in developing reactor protection system (RPS) for nuclear power plants in Korea, and experience to date has been positive. We demonstrate the proposed approach using the fixed set-point rising trip which is one of the most complex trip logics included in the RPS

  10. Design and Implementation of Electrostatic Spraying Automatic Controlling System Based on PLC

    Directory of Open Access Journals (Sweden)

    Weidong Jia

    2013-05-01

    Full Text Available The objective of this study was to improve the spraying efficiency and meet the demand of modern agricultural. A new generation of electrostatic sprayer which we designed realized the goals. The automatic controlling system is successfully designed. PLC (Programmable Logic Controller was taken as the control core of the system and LCD touch screen was employed for human-computer interaction interface. The system integrates kinds of techniques including programming, pressure monitoring and sensor technology, etc. The main structure of this equipment, working principle and control system hardware selection will be also introduced in the study. Human-computer interaction software was programmed by the software of Pro Tool/Pro CS. System controlling software was programmed in form of ladder diagram, which realized kinds of functions including ESD protection, accurate quantification, automatic controlling and humanized operation. Test results show that the effective spraying range is between 5 to 6 m, the Volume Median Diameter (VMD is 47.48 µm and the Ultra-Low Volume spray (ULV is realized. The spray deposition rate and effective availability of pesticide is higher than old sprayer. And also this new sprayer runs steadily.

  11. TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch.

    Science.gov (United States)

    Jian, Tunyu; Yang, Niuniu; Yang, Yan; Zhu, Chan; Yuan, Xiaolin; Yu, Guang; Wang, Changming; Wang, Zhongli; Shi, Hao; Tang, Min; He, Qian; Lan, Lei; Wu, Guanyi; Tang, Zongxiang

    2016-01-01

    Histamine H4 receptor has been confirmed to play a role in evoking peripheral pruritus. However, the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion (DRG) neurons is still unknown. By using cell culture and calcium imaging, we studied the underlying mechanism of activation of H4 receptor on the DRG neuron. Immepip dihydrobromide (immepip)-a histamine H4 receptor special agonist under cutaneous injection-obviously induced itch behavior of mice. Immepip-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor U73122. Application of immepip (8.3-50 μM) could also induce a dose-dependent increase in intracellular Ca(2+) ([Ca(2+)]i) of DRG neurons. We found that 77.8% of the immepip-sensitized DRG neurons respond to the TRPV1 selective agonist capsaicin. U73122 could inhibit immepip-induced Ca(2+) responses. In addition, immepip-induced [Ca(2+)]i increase could be blocked by ruthenium red, capsazepine, and AMG9810; however it could not be blocked by TRPA1 antagonist HC-030031. These results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons' responses in the downstream signaling pathway of histamine H4 receptor and suggest that TRPV1 may be involved in the mechanism of histamine-induced itch response by H4 receptor activation. PMID:26819760

  12. Advanced Technology of Automated Storage and Retrieval System Using PLC Integration

    Directory of Open Access Journals (Sweden)

    Ashna Joy

    2014-03-01

    Full Text Available This article proposes an highly developed fully automatic ASRS (automatic storage and retrieval system. This main material management support system is generally used for storing and retrieving the unprocessed materials in the manufacturing unit. The necessities of ASRS are increasingly of a more dynamic nature for which new models will need to be developed to overcome the time consumption. ASRS consists of a range of controlled systems for automatically retrieving and storing loads from preferred storage spaces. It is usually used in applications where there is a very high quantity of loads being moved into and out of storage. This system is mainly used for storing and retrieving the unprocessed materials in a manufacturing unit. The main aim of this paper is to build up an automatic system from the present system. The original idea of the article is to help public those who work as operators by designing a system where it is capable of managing the drawer without interference of an operator. The performance of the present system is enhanced by using PLC integration where it coordinates the operation and control of ASRS.

  13. Remediation of old environmental liabilities in the Nuclear Research Institute Rez plc

    International Nuclear Information System (INIS)

    The Nuclear Research Institute Rez plc (NRI) after 55 years of activities in the nuclear field produced some environmental liabilities that shall be remedied. There are three areas of remediation: (1) decommissioning of old obsolete facilities (e.g. decay tanks, RAW treatment technology, special sewage system), (2) processing of RAW from operation and dismantling of nuclear facilities, and (3) elimination of spent fuel from research nuclear reactors operated by the NRI. The goal is to remedy the environmental liabilities and eliminate the potential negative impact on the environment. Remediation of the environmental liabilities started in 2003 and will be finished in 2014. The character of the environmental liabilities is very specific and requires special remediation procedures. Special technologies are being developed with assistance of external subcontractors. The NRI has gained many experiences in the field of RAW management and decommissioning of nuclear facilities and will use its facilities, experienced staff and all relevant data needed for the successful realization of the remediation. The most significant items of environmental liabilities are described in the paper together with information about the history, the current state, the progress, and the future activities in the field of remediation of environmental liabilities in the NRI. (author)

  14. 汽车点灯仪中PLC的应用%Application of Automotive Lighting Instrument PLC

    Institute of Scientific and Technical Information of China (English)

    陈舜青; 过军

    2015-01-01

    通过对车灯测试仪功能的分析,文章阐述了应用可编程控制器技术、触摸屏技术实现车灯测试仪设计的方法。利用可编程控制器高速输入端与位移位指令,实现测量数据的采集与处理,利用触摸屏、可编程控制器及编程设计,实现新型号的自学习、测量控制、测量参数结果显示、型号输入与比对、信息存储、信息上传等功能。%Through the analysis of the function of the headlight tester. This paper introduces the application of programmable con⁃troller technology, touch screen technology design and implementation method of headlight tester. By using the programmable con⁃troller of high speed input and a shift instruction, realize the acquisition and processing of measurement data, using the touch screen, PLC programming and design, realization of self learning, measurement and control, measurement results show, model in⁃put and comparison, information storage, information upload and other functions of the new type.

  15. Inhibitory effect of genistein on PLC/PRF5 hepatocellular carcinoma cell line

    Directory of Open Access Journals (Sweden)

    Mehdi Nikbakht Dastjerdi

    2015-01-01

    Full Text Available Background: Natural compounds including flavonoids like genistein (GE are able to inhibit cell proliferation and induce apoptosis. GE is the main representative of these groups. GE inhibits carcinogenic tumors such as colon, stomach, lung, and pancreas tumors. The aim of the present study was to analyze the apoptotic effect of GE in the hepatocellular carcinoma (HCC PLC/PRF5 cell line. Methods: Cells were treated with various doses of GE (1, 5, 10, 25, 50, 75, and 100 μM/L at different times (24, 48, and 72 h and the MTT assay was commonly used. Furthermore, cells were treated with single dose of GE (25 μM at different times and flow cytometry was performed. Results: GE inhibited the growth of liver cancer cells significantly with a time- and dose-dependent manner. The percentage of living cells in GE treatment groups with a concentration of 25 μM at different times were 53, 48 and 47%, respectively (P < 0.001. Result of flow cytometry demonstrated that GE at a 25 μM concentration induces apoptosis significantly in a time-dependent manner. The percentage of apoptotic cells at different times were 44, 56, and 60%, respectively (P < 0.001. Conclusions: GE can significantly inhibit the growth of HCC cells and plays a significant role in apoptosis of this cell line.

  16. TRPV1 and PLC Participate in Histamine H4 Receptor-Induced Itch

    Directory of Open Access Journals (Sweden)

    Tunyu Jian

    2016-01-01

    Full Text Available Histamine H4 receptor has been confirmed to play a role in evoking peripheral pruritus. However, the ionic and intracellular signaling mechanism of activation of H4 receptor on the dorsal root ganglion (DRG neurons is still unknown. By using cell culture and calcium imaging, we studied the underlying mechanism of activation of H4 receptor on the DRG neuron. Immepip dihydrobromide (immepip—a histamine H4 receptor special agonist under cutaneous injection—obviously induced itch behavior of mice. Immepip-induced scratching behavior could be blocked by TRPV1 antagonist AMG9810 and PLC pathway inhibitor U73122. Application of immepip (8.3–50 μM could also induce a dose-dependent increase in intracellular Ca2+ (Ca2+i of DRG neurons. We found that 77.8% of the immepip-sensitized DRG neurons respond to the TRPV1 selective agonist capsaicin. U73122 could inhibit immepip-induced Ca2+ responses. In addition, immepip-induced Ca2+i increase could be blocked by ruthenium red, capsazepine, and AMG9810; however it could not be blocked by TRPA1 antagonist HC-030031. These results indicate that TRPV1 but not TRPA1 is the important ion channel to induce the DRG neurons’ responses in the downstream signaling pathway of histamine H4 receptor and suggest that TRPV1 may be involved in the mechanism of histamine-induced itch response by H4 receptor activation.

  17. A Study on the Optimal Receiver Impedance for SNR Maximization in Broadband PLC

    Directory of Open Access Journals (Sweden)

    Massimo Antoniali

    2013-01-01

    Full Text Available We consider the design of the front-end receiver for broadband power line communications. We focus on the design of the input impedance that maximizes the signal-to-noise ratio (SNR at the receiver. We show that the amplitude, rather than the power, of the received signal is important for communication purposes. Furthermore, we show that the receiver impedance impacts the amplitude of the noise term. We focus on the background noise, and we propose a novel description of the noise experienced at the receiver port of a PLC network. We model the noise as the sum of four uncorrelated contributions, that is, the active, resistive, receiver, and coupled noise components. We study the optimal impedance design problem for real in-home grids that we assessed with experimental measurements. We describe the results of the measurement campaign, and we report the statistics of the optimal impedance. Hence, we study the best attainable performance when the optimal receiver impedance is deployed. We focus on the SNR and the maximum achievable rate, and we show that power matching is suboptimal with respect to the proposed impedance design approach.

  18. Encoding Standards for Linguistic Corpora.

    Science.gov (United States)

    Ide, Nancy

    The demand for extensive reusability of large language text collections for natural languages processing research requires development of standardized encoding formats. Such formats must be capable of representing different kinds of information across the spectrum of text types and languages, capable of representing different levels of…

  19. Histamine acting on H1 receptor promotes inhibition of proliferation via PLC, RAC, and JNK-dependent pathways

    International Nuclear Information System (INIS)

    It is well established that histamine modulates cell proliferation through the activation of the histamine H1 receptor (H1R), a G protein-coupled receptor (GPCR) that is known to couple to phospholipase C (PLC) activation via Gq. In the present study, we aimed to determine whether H1R activation modulates Rho GTPases, well-known effectors of Gq/G11-coupled receptors, and whether such modulation influences cell proliferation. Experiments were carried out in CHO cells stably expressing H1R (CHO-H1R). By using pull-down assays, we found that both histamine and a selective H1R agonist activated Rac and RhoA in a time- and dose-dependent manner without significant changes in the activation of Cdc42. Histamine response was abolished by the H1R antagonist mepyramine, RGS2 and the PLC inhibitor U73122, suggesting that Rac and RhoA activation is mediated by H1R via Gq coupling to PLC stimulation. Histamine caused a marked activation of serum response factor activity via the H1R, as determined with a serum-responsive element (SRE) luciferase reporter, and this response was inhibited by RhoA inactivation with C3 toxin. Histamine also caused a significant activation of JNK which was inhibited by expression of the Rac-GAP β2-chimaerin. On the other hand, H1R-induced ERK1/2 activation was inhibited by U73122 but not affected by C3 or β2-chimaerin, suggesting that ERK1/2 activation was dependent on PLC and independent of RhoA or Rac. [3H]-Thymidine incorporation assays showed that both histamine and the H1R agonist inhibited cell proliferation in a dose-dependent manner and that the effect was independent of RhoA but partially dependent on JNK and Rac. Our results reveal that functional coupling of the H1R to Gq-PLC leads to the activation of RhoA and Rac small GTPases and suggest distinct roles for Rho GTPases in the control of cell proliferation by histamine.

  20. Histamine acting on H1 receptor promotes inhibition of proliferation via PLC, RAC, and JNK-dependent pathways

    Energy Technology Data Exchange (ETDEWEB)

    Notcovich, Cintia [Laboratorio de Patologia y Farmacologia Molecular, Instituto de Biologia y Medicina Experimental (Argentina); Laboratorio de Farmacologia de Receptores, Catedra de Quimica Medicinal, Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Diez, Federico [Laboratorio de Farmacologia de Receptores, Catedra de Quimica Medicinal, Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Tubio, Maria Rosario [Laboratorio de Patologia y Farmacologia Molecular, Instituto de Biologia y Medicina Experimental (Argentina); Laboratorio de Farmacologia de Receptores, Catedra de Quimica Medicinal, Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Baldi, Alberto [Laboratorio de Patologia y Farmacologia Molecular, Instituto de Biologia y Medicina Experimental (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Kazanietz, Marcelo G. [Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104 (United States); Davio, Carlos [Laboratorio de Farmacologia de Receptores, Catedra de Quimica Medicinal, Departamento de Farmacologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Shayo, Carina, E-mail: cshayo@dna.uba.ar [Laboratorio de Patologia y Farmacologia Molecular, Instituto de Biologia y Medicina Experimental (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina)

    2010-02-01

    It is well established that histamine modulates cell proliferation through the activation of the histamine H1 receptor (H1R), a G protein-coupled receptor (GPCR) that is known to couple to phospholipase C (PLC) activation via Gq. In the present study, we aimed to determine whether H1R activation modulates Rho GTPases, well-known effectors of Gq/G{sub 11}-coupled receptors, and whether such modulation influences cell proliferation. Experiments were carried out in CHO cells stably expressing H1R (CHO-H1R). By using pull-down assays, we found that both histamine and a selective H1R agonist activated Rac and RhoA in a time- and dose-dependent manner without significant changes in the activation of Cdc42. Histamine response was abolished by the H1R antagonist mepyramine, RGS2 and the PLC inhibitor U73122, suggesting that Rac and RhoA activation is mediated by H1R via Gq coupling to PLC stimulation. Histamine caused a marked activation of serum response factor activity via the H1R, as determined with a serum-responsive element (SRE) luciferase reporter, and this response was inhibited by RhoA inactivation with C3 toxin. Histamine also caused a significant activation of JNK which was inhibited by expression of the Rac-GAP {beta}2-chimaerin. On the other hand, H1R-induced ERK1/2 activation was inhibited by U73122 but not affected by C3 or {beta}2-chimaerin, suggesting that ERK1/2 activation was dependent on PLC and independent of RhoA or Rac. [{sup 3}H]-Thymidine incorporation assays showed that both histamine and the H1R agonist inhibited cell proliferation in a dose-dependent manner and that the effect was independent of RhoA but partially dependent on JNK and Rac. Our results reveal that functional coupling of the H1R to Gq-PLC leads to the activation of RhoA and Rac small GTPases and suggest distinct roles for Rho GTPases in the control of cell proliferation by histamine.

  1. Encoding, symbolic dynamics, cryptography and C++ implementations

    International Nuclear Information System (INIS)

    We investigate three techniques which can be used to encode information using certain types of chaotic maps and provide implementations in C++. One of the three, the variable bit length encoding technique, is a new technique for encoding information using transient chaos. We describe all three techniques, including variable bit length encoding, in detail. Finally we investigate the use of the variable bit length encoding technique as a cryptographic scheme

  2. Expression pattern of FGFR2, Grb2 and Plcγ1 acts as a novel prognostic marker of recurrence recurrence-free survival in lung adenocarcinoma

    Science.gov (United States)

    Timsah, Zahra; Berrout, Jonathan; Suraokar, Milind; Behrens, Carmen; Song, Juhee; Lee, J Jack; Ivan, Cristina; Gagea, Mihai; Shires, Michael; Hu, Xin; Vallien, Courtney; Kingsley, Charles V; Wistuba, IgnacioI; Ladbury, John E

    2015-01-01

    Lung adenocarcinoma is characterized by complex biology involving alterations at the genomic and protein expression levels. FGFR2 mutation and/or amplification are key drivers of disease progression and drug resistance in lung adenocarcinoma patients. These genetic alterations drive oncogenic downstream signalling due to the deregulated activity of the receptor. We have previously reported that wild type FGFR2 provides a binding site for which two proteins, Grb2 and Plcγ1, compete in a concentration-dependent manner. Metastasis and invasion ensue when Plcγ1 prevails on the receptor giving rise to oncogenic outcome in the absence of gene mutation/deletion. The effect of this signalling mechanism on FGFR2-driven lung adenocarcinoma has not previously been considered. In this study we show that fluctuation in the combinatorial expression levels of FGFR2, Grb2 and Plcγ1 modulates cell invasive properties, tumor formation and is linked to recurrence-free survival in 150 lung adenocarcinoma patients. High levels of expression of FGFR2 and Plcγ1 in a low background of Grb2 significantly correlates with poor prognosis. On the other hand, low levels of expression of FGFR2 and Plcγ1 in a high background of Grb2 correlates with favourable prognosis. This study defines the expression pattern of FGFR2, Plcγ1 and Grb2 as a novel prognostic marker in human lung adenocarcinoma. Thus, consideration of the Grb2 and Plcγ1-mediated mechanism of FGFR2 regulation will enhance the therapeutic targeting of aberrant FGFR2 activity to provide the much-needed improvement to the treatment regimen of this high mortality disease. PMID:26693065

  3. Synaptic encoding of temporal contiguity

    OpenAIRE

    Srdjan Ostojic; Stefano Fusi

    2013-01-01

    Often we need to perform tasks in an environment that changes stochastically. In these situations it is important to learn the statistics of sequences of events in order to predict the future and the outcome of our actions. The statistical description of many of these sequences can be reduced to the set of probabilities that a particular event follows another event (temporal contiguity). Under these conditions, it is important to encode and store in our memory these transition probabilities. ...

  4. SNOMED CT® Encoded Cancer Protocols

    OpenAIRE

    van Berkum, Monique M.

    2003-01-01

    SNOMED Clinical Terms® (SNOMED CT®) is being used to encode the Cancer Protocols published by the College of American Pathologists (CAP). As of January 1, 2004, one of the standards set for approved cancer programs by the American College of Surgeons Commission on Cancer will be that at least 90% of surgical pathology reports contain all essential data elements identified in the CAP Cancer Protocols.

  5. Design of test system for PLC input-output interfaces%PLC输入输出接口测试系统设计

    Institute of Scientific and Technical Information of China (English)

    车畅; 胡丹; 白晗东

    2011-01-01

    This paper describes a hardware circuit implemental method for testing peripheral equipment and PLC input-output interfaces with the host computer and the STC12C5410AD MCU.Furthermore, it introduced the program design of MCU and that of the host computer. And it proposed analog/digital input needed for the local PLC adjustment through simulation. At the same time, the designed testing system can receive the digital and analog signals from the PLC system.The testing system can simulate industry environment, be used to adjust the PLC system, and be convenient for PLC teaching and training.%详细介绍了一种基于STC12C5410AD单片机和上位机软件的PLC自检测和外围设备测试系统的硬件电路实现办法.以及单片机软件和上位机软件的实现方法,模拟设计出了PLC现场调试过程中所需要的模拟/数字输入,并且该测试系统的端口可以接受来自PLC的模拟/数字输出.该系统可以模拟工业现场环境,对PLC系统进行调试.另外也可对PLC的教学和培训提供较大便利.

  6. Modbus Protocol Monitoring Instrument for Industrial PLC%Modbus协议的工业PLC监控仪表设计

    Institute of Scientific and Technical Information of China (English)

    李振东

    2012-01-01

    本监控仪表基于Modbus协议设计,采用STC12C5A60S2高速单片机,与西门子工业PLC通信实现PLC控制与过程数据的实时在线读写显示与报警,并为工业DCS系统提供远程4~20mA工业标准电流信号输出。工作时仪表为Modbus主站,PLC为Modbus从站。采用Modbus协议,还可使本仪表用于其他支持Modbus协议的各种工业控制设备的监控。本文介绍了仪表的主要电路设计,剖析了Modbus协议细节,并给出了仪表Modbus软件的实现思路及程序代码。%The monitoring instrument is designed by STC12C5A60S2 high-speed MCU based on Modbus protocol. It can communicate with the Siemens industrial PLC, realize PLC control and process data real-time online read-write display and alarm, and provide longdistance 4-20 mA industrial standard current signal output for industrial DCS system. The instrument works as a Modbus master, while the PLC as a slave. Using of the Modbus protocol also makes the instrument monitor other various industrial control equipments support- ing the Modbus protocol. This paper describes the main instrument circuit design, reveals the detail of the Modbus protocol, and presents the instrument Modbus software scheme and program code.

  7. The influence of the chemical composition and temperature of plastic deformation on the PLC effect in tin bronzes

    Directory of Open Access Journals (Sweden)

    W. Ozgowicz

    2006-04-01

    Full Text Available Purpose: The aim of the present paper is the determination of the effect of the chemical composition and temperature of deformation of standardized tin bronzes and bronze modified with zirconium on the Portevin – Le Chatelier (PLC phenomenon, mainly basing on the shape of stress-strain curves within the temperature range of 100÷300oC and observations of their structure. The reasons of the occurrence of such en effect are so far no fully known and explained and the opinions concerning is physical basis vary.Design/methodology/approach: Of essential design in this research is determination of the dependence of PLC effect on the chemical composition, temperature and strain rate and the preliminary heat treatment and grain size. The main method used in this investigation is tensile test at elevated temperature.Findings: The main conclusions are following: the PLC effect on tin bronzes with a miero-addition of zirconium in an amount of 0.01÷0.05% depends the temperature of deformation in the tensile test in the range of 100÷300°C and the chemical composition of the alloys; the type of serration revealed on the δ–ε curve depend mainly on the temperature of deformation and can be differ during the respective stages of the analyzed curves.Practical implications: In this paper implications for practice are not taken into consideration.Originality/value: In this paper an additive type of serration observed on the curves δ–ε, denoted by the symbol D, is new.

  8. PLC控制槽针运动设计%Design the movement of groove with the control of PLC

    Institute of Scientific and Technical Information of China (English)

    钱秋波; 王传洋

    2011-01-01

    Though groove-needle and needle-core are respectively driven by different original dynamic parts, their mutual movements can help the warp knitting machine realize different controlling goals.Servo drive will replace the traditional cam-drive.Since trajectory of groove needlepoint is already known,through the institutional links the mathematical expression between two original dynamic parts and the coordinates of groove needlepoint can be deduced.Construct the hardware control system for controlling,and the PLC procedures are prepared to control the corners of servo motors ,so the input shaft is directly driven by PLC servo.When producing different kinds of products ,only PLC procedures need to be changed.In this way,the goal of control automation can be reached.%经编机是依靠由槽针与针芯之间的相互运动关系,从而达到不同的控制运动,而槽针与针芯的运动是由不同的原动件所驱动的.采用伺服驱动来代替传统的凸轮驱动,由已知槽针针尖的运动轨迹,通过机构之间的联接关系,推导出两原动件与槽针针尖坐标之间的数学表达式,构建了控制的硬件系统,并且通过编写PLC的程序来控制伺服电机的转角,由PLC伺服直接驱动输入轴运动.不同类型的产品只需要改变PLC的程序,即可达到灵活控制的目的,实现控制的自动化.

  9. Hall effect encoding of brushless dc motors

    Science.gov (United States)

    Berard, C. A.; Furia, T. J.; Goldberg, E. A.; Greene, R. C.

    1970-01-01

    Encoding mechanism integral to the motor and using the permanent magnets embedded in the rotor eliminates the need for external devices to encode information relating the position and velocity of the rotating member.

  10. Early LPS-induced ERK activation in retinal pigment epithelium cells is dependent on PIP 2 -PLC.

    Science.gov (United States)

    Mateos, Melina V; Kamerbeek, Constanza B; Giusto, Norma M; Salvador, Gabriela A

    2016-06-01

    This article presents additional data regarding the study "The phospholipase D pathway mediates the inflammatory response of the retinal pigment epithelium" [1]. The new data presented here show that short exposure of RPE cells to lipopolysaccharide (LPS) induces an early and transient activation of the extracellular signal-regulated kinase (ERK1/2). This early ERK1/2 activation is dependent on phosphatidylinositol bisphosphate-phospholipase C (PIP2-PLC). On the contrary, neither the phospholipase D 1 (PLD1) nor the PLD2 inhibition is able to modulate the early ERK1/2 activation induced by LPS in RPE cells. PMID:27006973

  11. Correlation of a subset of the pLC plasmids to the physical map of Escherichia coli K-12.

    OpenAIRE

    Nishimura, A.; Akiyama, K.; Kohara, Y.; Horiuchi, K

    1992-01-01

    We determined map positions of the Escherichia coli K-12 portions of a subset of the hybrid E. coli-ColE1 plasmids constructed by Clarke and Carbon. The probe DNA of pLC plasmids was labeled with digoxigenine-dUTP, hybridized to the 476 phage clones of the E. coli ordered clone bank miniset, which was adsorbed on a strip of nylon membrane filters, and detected by enzyme-linked immunoassay and a subsequent enzyme-catalyzed color reaction. The total number of Clarke-Carbon plasmids we analyzed ...

  12. Design and Implementation of Control and Monitoring Systems Based on HMI-PLC for Potable Water Well

    Directory of Open Access Journals (Sweden)

    Quezada-Quezada José Carlos

    2014-01-01

    Full Text Available This project reports on the design and implementation in a workbench of a control and monitoring system of the discharge of water of a well. Graphic User's Interfaces (GUI are designed for interaction with the operator. The Human Machine Interface (HMI was implement in proprietor software and it contemplates the rules for control and monitoring of the conditions of the system for the operator, the HMI is also interconnected a Programmable Logic Controller (PLC where the rules of protection of the process are implemented in Ladder Diagram (LD.

  13. Development of Real Time Operating System for Safety Grade PLC (POSAFE-Q) for Nuclear Power Plants

    International Nuclear Information System (INIS)

    POSAFE-Q is a newly developed programmable logic controller (PLC) in order to apply to digital safety system of nuclear power plants (NPP) according to Nuclear Power Plant safety requirements. POSAFE-Q hardware and software development process, including design, review, verification and validation, and configuration control and quality assurance, satisfies the requirements imposed by 10CFR50, Appendix B. This article introduces a real time operating system pCOS, which is the core of POSAFE-Q. Section 2 describes the structure of pCOS. Section 3 describes a few important features of pCOS, which are necessary to the application for the digital safety system of NPP

  14. Variational Recurrent Auto-Encoders

    OpenAIRE

    Fabius, Otto; van Amersfoort, Joost R.

    2014-01-01

    In this paper we propose a model that combines the strengths of RNNs and SGVB: the Variational Recurrent Auto-Encoder (VRAE). Such a model can be used for efficient, large scale unsupervised learning on time series data, mapping the time series data to a latent vector representation. The model is generative, such that data can be generated from samples of the latent space. An important contribution of this work is that the model can make use of unlabeled data in order to facilitate supervised...

  15. Comfortable district heating by ingenious PLC-control. Solar heat for district heating and hot water; Comfortabele stadswarmte dankzij ingenieuze PLC-regeling. Zonnewarmte voor stadverwarming en warm tapwater

    Energy Technology Data Exchange (ETDEWEB)

    Bezemer, R. [Bezemer Communicatie, Brummen (Netherlands)

    2010-07-15

    Over 45,000 dwellings in Almere, the Netherlands, are connected to a district heating grid. The energy supplier uses residual heat that is released by a CHP plant in Almere Stad. As of recently, 'Zoneiland Almere' (1.5 ha in size, 7,000 m{sup 2} of solar collectors) is supplying about 9,750 GJ/a. This covers about 10% of the energy demand of a district with about 2,700 dwellings. PLC is the abbreviation of Product-Logic-Circuit. [Dutch] Meer dan 45.000 woningen in Almere zijn aangesloten op een stadswarmte netwerk. De energieleverancier gebruikt restwarmte die vrijkomt bij een warmtekrachtcentrale in Almere Stad. Sinds kort levert 'Zoneiland Almere' (1,5 ha groot, met daarop 7.000 m{sup 2} zonnecollectoren) circa 9.750 GJ/a. Dit komt overeen met 10 procent van de energiebehoefte van een wijk met 2.700 woningen. PLC staat voor Product-Logic-Circuit.

  16. Metodología para la implementación de controlador difuso tipo Takagi-Sugeno en PLC s7-300

    Directory of Open Access Journals (Sweden)

    Cristian Guarnizo Lemus

    2011-12-01

    Full Text Available This paper presents a methodology for implementing fuzzy logic controllers based on a S7-300 PLC using the programming language SCL(Structured Control Language in STEP 7. We present the design of the fuzzy function, variable declarations, and the evaluation design of membership functions and rules of the fuzzy system. Since this scheme can be implemented more complex fuzzy models, such as adaptive or Autotuner. We present an application example for a discrete time simulated in the PLC using a PI fuzzy controller.

  17. Contractive De-noising Auto-encoder

    OpenAIRE

    Chen, Fu-qiang; Wu, Yan; Zhao, Guo-dong; Zhang, Jun-Ming; Zhu, Ming; Bai, Jing

    2013-01-01

    Auto-encoder is a special kind of neural network based on reconstruction. De-noising auto-encoder (DAE) is an improved auto-encoder which is robust to the input by corrupting the original data first and then reconstructing the original input by minimizing the reconstruction error function. And contractive auto-encoder (CAE) is another kind of improved auto-encoder to learn robust feature by introducing the Frobenius norm of the Jacobean matrix of the learned feature with respect to the origin...

  18. Development of PLC based control system for frequency tuning loop of 31.6 MHz RF cavity

    International Nuclear Information System (INIS)

    Two storage rings, Indus-1 and Indus-2 are operational at RRCAT. Both the storage rings share a common injector system consisting of a Microtron and a Booster Synchrotron. The electrons are generated and accelerated to 20 MeV in Microtron and injected through a transfer line into the Booster Synchrotron where its energy is increased to 450 MeV/550 MeV. In Booster Synchrotron, a MHz RF system is installed. This RF System includes 1 kW RF amplifier, re-entrant type RF cavity and its tuning system. The RF system works in ramp mode. In RF cavities, resonant frequency changes due to beam loading and temperature effect. Our aim is to keep the RF Cavity at fixed resonance. Frequency Tuning Loop (FTL) will keep the cavities tuned by compensating for both beam loading and temperature effects. This is realized by means of a mechanism, driven by a stepper motor, which changes the volume of RF cavity by moving the plunger in/out and therefore its frequency. A Programmable Logic Controller (PLC) based Control system was developed to replace existing control system for FTL of RF Cavity installed in Booster Synchrotron. The main components of the control system are the phase detector, PLC with analog module, optical isolation card and touch screen interface. Control logic was developed in Step-7 MicroWin. The complete control logic, advantages over the earlier control system and scope for adaptability has been thoroughly discussed in this paper

  19. Crystallization, optimization and preliminary X-ray characterization of a metal-dependent PI-PLC from Streptomyces antibioticus

    International Nuclear Information System (INIS)

    Crystallization and diffraction analysis of a Ca2+-dependent PI-PLC from Streptomyces is reported. Optimization of crystals was completed using a drop-pinning technique and heavy-atom soaks to achieve high-quality diffraction to 1.23 Å. A recombinant metal-dependent phosphatidylinositol-specific phospholipase C (PI-PLC) from Streptomyces antibioticus has been crystallized by the hanging-drop method with and without heavy metals. The native crystals belonged to the orthorhombic space group P222, with unit-cell parameters a = 41.26, b = 51.86, c = 154.78 Å. The X-ray diffraction results showed significant differences in the crystal quality of samples soaked with heavy atoms. Additionally, drop pinning, which increases the surface area of the drops, was also used to improve crystal growth and quality. The combination of heavy-metal soaks and drop pinning was found to be critical for producing high-quality crystals that diffracted to 1.23 Å resolution

  20. NuSEE: an integrated environment of software specification and V and V for PLC based safety-critical systems

    International Nuclear Information System (INIS)

    As the use of digital systems becomes more prevalent, adequate techniques for software specification and analysis have become increasingly important in Nuclear Power Plant (NPP) safety-critical systems. Additionally, the importance of software Verification and Validation (V and V) based on adequate specification has received greater emphasis in view of improving software quality. For thorough V and V of safety-critical systems, V and V should be performed throughout the software lifecycle. However, systematic V and V is difficult as it involves many manual-oriented tasks. Tool support is needed in order to more conveniently perform software V and V. In response, we developed four kinds of Computer Aided Software Engineering (CASE) tools to support system specification for a formal-based analysis according to the software lifecycle. In this work, we achieved optimized integration of each tool. The toolset, NuSEE, is an integrated environment for software specification and V and V for PLC based safety-critical systems. In accordance with the software lifecycle, NuSEE consists of NuSISRT for the concept phase, NuSRS for the requirements phase, NuSDS for the design phase and NuSCM for configuration management. It is believed that after further development our integrated environment will be a unique and promising software specification and analysis toolset that will support the entire software lifecycle for the development of PLC based NPP safety-critical systems

  1. Molecular mechanisms for protein-encoded inheritance

    Energy Technology Data Exchange (ETDEWEB)

    Wiltzius, Jed J.W.; Landau, Meytal; Nelson, Rebecca; Sawaya, Michael R.; Apostol, Marcin I.; Goldschmidt, Lukasz; Soriaga, Angela B.; Cascio, Duilio; Rajashankar, Kanagalaghatta; Eisenberg, David; (Cornell); (HHMI)

    2009-12-01

    In prion inheritance and transmission, strains are phenotypic variants encoded by protein 'conformations'. However, it is unclear how a protein conformation can be stable enough to endure transmission between cells or organisms. Here we describe new polymorphic crystal structures of segments of prion and other amyloid proteins, which offer two structural mechanisms for the encoding of prion strains. In packing polymorphism, prion strains are encoded by alternative packing arrangements (polymorphs) of {beta}-sheets formed by the same segment of a protein; in segmental polymorphism, prion strains are encoded by distinct {beta}-sheets built from different segments of a protein. Both forms of polymorphism can produce enduring conformations capable of encoding strains. These molecular mechanisms for transfer of protein-encoded information into prion strains share features with the familiar mechanism for transfer of nucleic acid-encoded information into microbial strains, including sequence specificity and recognition by noncovalent bonds.

  2. Identification of single nucleotide polymorphisms associated with hyperproduction of alpha-toxin in Staphylococcus aureus.

    Directory of Open Access Journals (Sweden)

    Xudong Liang

    Full Text Available The virulence factor α-toxin (hla is needed by Staphylococcus aureus in order to cause infections in both animals and humans. Although the complicated regulation of hla expression has been well studied in human S. aureus isolates, the mechanisms of of hla regulation in bovine S. aureus isolates remain undefined. In this study, we found that many bovine S. aureus isolates, including the RF122 strain, generate dramatic amounts of α-toxin in vitro compared with human clinical S. aureus isolates, including MRSA WCUH29 and MRSA USA300. To elucidate potential regulatory mechanisms, we analyzed the hla promoter regions and identified predominant single nucleotide polymorphisms (SNPs at positions -376, -483, and -484 from the start codon in α-toxin hyper-producing isolates. Using site-directed mutagenesis and hla promoter-gfp-luxABCDE dual reporter approaches, we demonstrated that the SNPs contribute to the differential control of hla expression among bovine and human S. aureus isolates. Using a DNA affinity assay, gel-shift assays and a null mutant, we identified and revealed that an hla positive regulator, SarZ, contributes to the involvement of the SNPs in mediating hla expression. In addition, we found that the bovine S. aureus isolate RF122 exhibits higher transcription levels of hla positive regulators, including agrA, saeR, arlR and sarZ, but a lower expression level of hla repressor rot compared to the human S. aureus isolate WCUH29. Our results indicate α-toxin hyperproduction in bovine S. aureus is a multifactorial process, influenced at both the genomic and transcriptional levels. Moreover, the identification of predominant SNPs in the hla promoter region may provide a novel method for genotyping the S. aureus isolates.

  3. 太阳能低压果蔬干燥机的 PLC 控制系统设计%The PLC Control System Design of the Solar Energy Low-voltage Fruit Vegetable Drier

    Institute of Scientific and Technical Information of China (English)

    付丽娟; 张恒; 苏有刚

    2013-01-01

      目前我国已成为世界干果蔬生产和出口的主要国家之一,但是果蔬脱水烘干装置存在加工工艺落后、产品质量较低及能耗大等问题,严重影响经济效益。为此,提出了基于 PLC 控制的太阳能低压果蔬干燥机的设计方案。该方法主要利用太阳能来提供设备能源,在低于50℃的环境下让果蔬近似自然脱水,达到节能环保和降低成本的目的。利用 PLC控制器、干燥箱、增压泵、真空泵、电磁阀和传感器等器件搭建实验样机,初步实验结果表明,太阳能低压果蔬干燥机生产的脱水果蔬具有品质好、色泽好和产品等级高等优点。同时,设备的制造市场经济规模非常可观,产品具有很好的市场前景,极具推广价值。%At present, China has become one of the major countries in the world , which produce and export fruit and vegetable directly , but the present fruit and vegetable drier has several problems that backward processing , lower product quality , energy consumption , which serious impact on economic efficiency .In view of this status , it proposed the re-search of the PLC based solar energy low-voltage fruit vegetable drier .This method mainly makes use of the solar energy to provide equipment energy , and let fruits and vegetables natural dehydrated in below 50℃ an environment to achieve energy saving and reduce costs .It has been built the experimental prototype , which contained the PLC controller ,a drier, a booster pump , a vacuum pump , solenoid valves and sensors etc .Through do the experiment , the preliminary results show that the dehydrated fruits and vegetables production of solar energy fruit vegetable dryer have good quality , good col-or , high grade product advantages .And it has very substantial economies scale for the equipment manufacturing market , so the product has good market prospects and great promotional value .

  4. 75 FR 51654 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-22B and RB211-524 Series Turbofan Engines

    Science.gov (United States)

    2010-08-23

    ... Register on May 19, 2010 (75 FR 27964). That NPRM proposed to correct an unsafe condition for the specified... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...-26-AD; Amendment 39-16402; AD 2010-17-10] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  5. 75 FR 27964 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-22B and RB211-524 Series Turbofan Engines

    Science.gov (United States)

    2010-05-19

    ... Register published on April 11, 2000 (65 FR 19477-78). Discussion The European Aviation Safety Agency (EASA... Policies and Procedures (44 FR 11034, February 26, 1979); and 3. Will not have a significant economic... Federal Aviation Administration 14 CFR Part 39 RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  6. 76 FR 24798 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-Trent 875-17, RB211-Trent 877-17, RB211...

    Science.gov (United States)

    2011-05-03

    ..., 2011 (76 FR 2605). That NPRM proposed to correct an unsafe condition for the specified products. The... Order 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR...-30-AD; Amendment 39-16657; AD 2011-08-07] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  7. 76 FR 24793 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series and RB211 Trent 500, 700, and 800...

    Science.gov (United States)

    2011-05-03

    ... (75 FR 33738). That NPRM proposed to correct an unsafe condition for the specified products. The MCAI... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034...-29-AD; Amendment 39-16669; AD 2011-09-07] RIN 2120-AA64 Airworthiness Directives; Rolls-Royce plc...

  8. 75 FR 33738 - Airworthiness Directives; Rolls-Royce plc (RR) RB211-524 Series and RB211 Trent 500, 700, and 800...

    Science.gov (United States)

    2010-06-15

    ... complete Privacy Act Statement in the Federal Register published on April 11, 2000 (65 FR 19477-78... 12866; 2. Is not a ``significant rule'' under the DOT Regulatory Policies and Procedures (44 FR 11034... plc (RR) RB211-524 Series and RB211 Trent 500, 700, and 800 Series Turbofan Engines AGENCY:...

  9. Influence of Professional Learning Community (PLC) on Learning a Constructivist Teaching Approach (POE): A Case of Secondary Science Teachers in Bangladesh

    Science.gov (United States)

    Rahman, S. M. Hafizur

    2012-01-01

    No major change has occurred up until now with regard to the teaching-learning methods of science used in Bangladesh. Teachers, in most cases, tend to teach the same things in the same ways they were taught when they were students. This study will, therefore, investigate how science teachers' learning in a professional learning community (PLC)…

  10. Leishmania amazonensis: heme stimulates (Na(+)+K(+))ATPase activity via phosphatidylinositol-specific phospholipase C/protein kinase C-like (PI-PLC/PKC) signaling pathways.

    Science.gov (United States)

    Almeida-Amaral, Elmo Eduardo; Cardoso, Viviane Carrozino; Francioli, Fernanda Gomes; Meyer-Fernandes, José Roberto

    2010-04-01

    In the present paper we studied the involvement of the phosphatidylinositol-specific PLC (PI-PLC)/protein kinase C (PKC) pathway in (Na(+)+K(+))ATPase stimulation by heme in Leishmania amazonensis promastigotes. Heme stimulated the PKC-like activity with a concentration of 50nM. Interestingly, the maximal stimulation of the PKC-like activity promoted by phorbol ester was of the same magnitude promoted by heme. However, the stimulatory effect of heme is completely abolished by ET-18-OCH(3) and U73122, specific inhibitors of PI-PLC. (Na(+)+K(+))ATPase activity is increased in the presence of increased concentrations of heme, being maximally affected at 50nM. This effect was completely reversed by 10nM calphostin C, an inhibitor of PKC. Thus, the effect of 50nM heme on (Na(+)+K(+))ATPase activity is completely abolished by ET-18-OCH(3) and U73122. Taken together, these results demonstrate that the heme receptor mediates the stimulatory effect of heme on the (Na(+)+K(+))ATPase activity through a PI-PLC/PKC signaling pathway. PMID:20045694

  11. KOGNAC: Efficient Encoding of Large Knowledge Graphs

    OpenAIRE

    Urbani, Jacopo; Dutta, Sourav; Gurajada, Sairam; Weikum, Gerhard

    2016-01-01

    Many Web applications require efficient querying of large Knowledge Graphs (KGs). We propose KOGNAC, a dictionary-encoding algorithm designed to improve SPARQL querying with a judicious combination of statistical and semantic techniques. In KOGNAC, frequent terms are detected with a frequency approximation algorithm and encoded to maximise compression. Infrequent terms are semantically grouped into ontological classes and encoded to increase data locality. We evaluated KOGNAC in combination w...

  12. Auto-encoders: reconstruction versus compression

    OpenAIRE

    Ollivier, Yann

    2014-01-01

    We discuss the similarities and differences between training an auto-encoder to minimize the reconstruction error, and training the same auto-encoder to compress the data via a generative model. Minimizing a codelength for the data using an auto-encoder is equivalent to minimizing the reconstruction error plus some correcting terms which have an interpretation as either a denoising or contractive property of the decoding function. These terms are related but not identical to those used in den...

  13. Computational Intelligence and Its Encoding Mechanism

    Institute of Scientific and Technical Information of China (English)

    LIUMan-dan

    2004-01-01

    The origin and characteristics of computational intelligence, and several typical computational intelligence algorithms such as genetic algorithm and DNA computing are described, and the influence of evolution strategies and convergence properties on the encoding mechanism is discussed. A novel genetic algorithm based on degressive carry number encoding is then proposed. This algorithm uses degressive carry number encoding in the evolutionary process instead of commonly used fixed carry number. Finally a novel encoding mechanism and a new algorithm are proposed, which combine modem computational intelligence with the traditional Chinese methodology.

  14. Computational Intelligence and Its Encoding Mechanism

    Institute of Scientific and Technical Information of China (English)

    LIU Man-dan

    2004-01-01

    The origin and characteristics of computational intelligence, and several typical computational intelligence algorithms such as genetic algorithm and DNA computing are described, and the influence of evolution strategies and convergence properties on the encoding mechanism is discussed. A novel genetic algorithm based on degressive carry number encoding is then proposed. This algorithm uses degressive carry number encoding in the evolutionary process instead of commonly used fixed carry number. Finally a novel encoding mechanism and a new algorithm are proposed, which combine modern computational intelligence with the traditional Chinese methodology.

  15. Rate-Distortion Auto-Encoders

    OpenAIRE

    Giraldo, Luis G. Sanchez; Principe, Jose C.

    2013-01-01

    A rekindled the interest in auto-encoder algorithms has been spurred by recent work on deep learning. Current efforts have been directed towards effective training of auto-encoder architectures with a large number of coding units. Here, we propose a learning algorithm for auto-encoders based on a rate-distortion objective that minimizes the mutual information between the inputs and the outputs of the auto-encoder subject to a fidelity constraint. The goal is to learn a representation that is ...

  16. Perceptually adapted MPEG video encoding

    Science.gov (United States)

    Bordes, Philippe; Guillotel, Philippe

    2000-06-01

    In picture quality assessment, the amount of distortion perceived by a human observer differs from one region to another according to its particular local content. This subjective perception can be explained/predicted by considering some simple psychovisual properties (masking) of the Human Visual System (HVS). We have implemented a HVS model based on a pyramid decomposition for extracting the spatial frequencies, associated with a multi-resolution motion representation. Then the visibility of the decoded errors is computed by exploiting the Kelly's contrast sensitivity spatio-velocity model. The resulting data is called a 'Quality-map.' Special attention has been paid to temporal/moving effects since, in the case of video sequences, motion strongly influences the subjective quality assessment. The quality of the motion information is thus preponderant. In the second part, two possible uses of these psychovisual properties for improving MPEG video encoding performances are depicted: (1) The pre-processing of the pictures to remove non-visible information using a motion adapted filtering. This process is efficient in term of bits saved and degradation is not significant especially on consumer electronic TV sets. (2) A perceptual quantizer based on a local adaptation scheme in order to obtain Quality-maps as uniform as possible (homogeneous perceived distortion), at constant bit-rate. Further improvements have been considered, especially when the viewer is tracking a moving object in the scene.

  17. STS-57 MS and PLC Low, in EMU and atop the RMS, is maneuvered in OV-105's PLB

    Science.gov (United States)

    1993-01-01

    This extravehicular activity (EVA) scene captured by one of the STS-57 crewmembers in Endeavour's, Orbiter Vehicle (OV) 105's, crew cabin shows Mission Specialist (MS) and Payload Commander (PLC) G. David Low conducting Detailed Test Objective 1210 procedures. Pictured near the recently 'captured' European Retrievable Carrier (EURECA) at frame center, Low, suited in an extravehicular mobility unit (EMU), is anchored to the remote manipulator system (RMS) via a portable foot restraint (PFR) (manipulator foot restraint (MFR)). The PFR is attached to the RMS end effector via a PFR attachment device (PAD). DTO 1210 results will assist in refining several procedures being developed to service the Hubble Space Telescope (HST) on mission STS-61 in December 1993. Partially visible in the foreground is the Superfluid Helium Onorbit Transfer (SHOOT) payload.

  18. PLC与变频器之间的Profibus-DP通讯%Profibus-DP Communication between the PLC and Inverter

    Institute of Scientific and Technical Information of China (English)

    张杰

    2014-01-01

    简述Profibus-DP现场总线的特点,研究PLC与变频器之间的DP网络结构,分析ABB变频器在西门子STEP7软件中的硬件组态配置和ABB变频器的通讯控制参数设定,为今后Profibus-DP现场总线的应用提供参考。%Brief DP Profibus-DP network structure characteristics fieldbus, PLC and inverter between research, analyzes ABB ABB inverter drive configuration and the hardware configuration of the communication control parameters set in the Siemens STEP7 software for provide a reference for future applications Profibus-DP fieldbus.

  19. A compact and low-loss 1×8 optical power splitter using silica-based PLC on quartz substrate

    Science.gov (United States)

    Wang, Liangliang; An, Junming; Wu, Yuanda; Zhang, Jiashun; Wang, Yue; Li, Jianguang; Wang, Hongjie; Zhang, Xiaoguang; Pan, Pan; Zhang, Liyao; Dai, Hongqing; Liu, Ruidan; Zhong, Fei; Zha, Qiang; Hu, Xiongwei; Zhao, Degang

    2014-02-01

    In this paper, a compact, low-loss and good-uniformity 1×8 optical power splitter with new Y-branch structure is demonstrated using silica-based PLC technology on quartz substrate. Broadening waveguide and transition waveguide of the new Y-branch are optimized by using 3D BPM. The measurement results show that the insertion loss (IL) and the uniformity (UNIF) of the splitters are less than 9.5 dB and 0.35 dB, respectively, in the wavelength range from 1.26 μm to 1.65 μm, and the total product ratio of premium grade in a whole 6 in. wafer is more than 95%. The results mean that our technology completely satisfies the need of mass manufacture.

  20. Development of Real Time Operating System for Safety Grade PLC (POSAFE-Q) for Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Son, Han Seong [ENESYS, Taejon (Korea, Republic of); Hwang, Sung Jae [POSCON, Seoul (Korea, Republic of); Lee, Young Joon; Kim, Chang Hwoi; Lee, Dong Young [Korea Atomic Energy Research Institute, Taejon (Korea, Republic of)

    2006-07-01

    POSAFE-Q is a newly developed programmable logic controller (PLC) in order to apply to digital safety system of nuclear power plants (NPP) according to Nuclear Power Plant safety requirements. POSAFE-Q hardware and software development process, including design, review, verification and validation, and configuration control and quality assurance, satisfies the requirements imposed by 10CFR50, Appendix B. This article introduces a real time operating system pCOS, which is the core of POSAFE-Q. Section 2 describes the structure of pCOS. Section 3 describes a few important features of pCOS, which are necessary to the application for the digital safety system of NPP.0.

  1. DNA encoding a DNA repair protein

    Science.gov (United States)

    Petrini, John H.; Morgan, William Francis; Maser, Richard Scott; Carney, James Patrick

    2006-08-15

    An isolated and purified DNA molecule encoding a DNA repair protein, p95, is provided, as is isolated and purified p95. Also provided are methods of detecting p95 and DNA encoding p95. The invention further provides p95 knock-out mice.

  2. A METHOD OF SHAPE ENCODING AND RETRIEVAL

    Institute of Scientific and Technical Information of China (English)

    Huang Xianglin; Song Lei; Shen Lansun

    2002-01-01

    A method of shape encoding and retrieval is proposed in this letter, which uses centripetal code to encode shape and extracts shape's convex for retrieval. For the rotation invariance and translation invariance of the centripetal code and the normalization of convex,the proposed retrieval method is similarity transform resistant, Experimental results confirm this capability.

  3. The Arabic Diatessaron Project: Digitalizing, Encoding, Lemmatization

    Directory of Open Access Journals (Sweden)

    Giuliano Lancioni

    2016-04-01

    Full Text Available The Arabic Diatessaron Project (henceforth ADP is an international research project in Digital Humanities that aims to collect, digitalise and encode all known manuscripts of the Arabic Diatessaron (henceforth AD, a text that has been relatively neglected in scholarly research. ADP’s final goal is to provide a number of tools that can enable scholars to effectively query, compare and investigate all known variants of the text that will be encoded as far as possible in compliance with the Text Encoding Initiative (TEI guidelines. The paper addresses a number of issues involved in the process of digitalising manuscripts included in the two existing editions (Ciasca 1888 and Marmardji 1935, adding variants in unedited manuscripts, encoding and lemmatising the text. Issues involved in the design of the ADP include presentation of variants, choice of the standard text, applicability of TEI guidelines, automatic translation between different encodings, cross-edition concordances and principles of lemmatisation.

  4. Distribution of Insertion- and Deletion-Associated Genetic Polymorphisms among Four Mycobacterium tuberculosis Phospholipase C Genes and Associations with Extrathoracic Tuberculosis: a Population-Based Study

    OpenAIRE

    Kong, Y.; Cave, M D; D. Yang; Zhang, L.; Marrs, C. F.; Foxman, B; Bates, J. H.; Wilson, F.; Mukasa, L. N.; Yang, Z H

    2005-01-01

    The Mycobacterium tuberculosis genome contains four phospholipase C (PLC)-encoding genes, designated plcA, plcB, plcC, and plcD, respectively. Each of the four genes contributes to the overall PLC activity of M. tuberculosis. PLC is hypothesized to contribute to M. tuberculosis virulence. Infection of M. tuberculosis strains carrying a truncated plcD gene is associated with the occurrence of extrathoracic tuberculosis. However, whether the other three plc genes are also associated with extrat...

  5. 气吸式自动穴盘育苗精量播种机设计-基于 PLC 控制%Design of Pneumatic Automatic Tray Precision Seeding Machine-Based on PLC

    Institute of Scientific and Technical Information of China (English)

    胡志新; 翁凌霄; 汪小志

    2016-01-01

    为了适应微小体积种子的穴盘育苗精量播种工作要求,提高精量播种机的普遍适应能力,基于PLC控制系统和传感器信号采集,采用压电弹簧和气吸盘相结合的方法,设计了一种新型自动穴盘精量播种机。利用压电弹簧对排种器振动系统和驱动系统进行了改进,基于逆压电效应,将微小位移放大后驱动排种器盘进行振动,使种子达到了理想的排种运动状态,大大提高了吸种效率和播种性能。试验结果表明:PLC 控制系统通过自适应调整可以使误差降低到接近于0,且响应迅速,响应精度较高,播种机的空穴率、多粒率和破碎率均不高于5%,而吸附率和播种合格率都在95%以上,满足穴盘育苗精量播种的农艺要求,达到了预期设计目标。%In order to adapt the work requirements of the small seed size hole tray precision seeding , improve the preci-sion seeding machine generally adapt ability , it uses piezoelectric spring and the gas sucking disc combined method based on PLC control system and sensor signal acquisition .The paper designs a new automatic tray precision seeding machine . The vibration system and the drive system of the vibration system are improved by using the piezoelectric spring , and the vibration of the drive plate is driven by the inverse piezoelectric effect , which makes the seeds reach the ideal seeding state .Planting performance of the prototype was tested .The experimental results show that through the adaptive adjust-ment the PLC control system can reduce the error to close to zero , and rapid response and high precision response , drill hole rate , grain rate and broken rate were not more than 5%, and adsorption of seeding rate and qualified rate are above 95%,which meets the tray precision seeding agronomic requirements , and achieve the expected design .

  6. Source Coding With Encoder Side Information

    OpenAIRE

    Martinian, Emin; Wornell, Gregory W.; Zamir, Ram

    2004-01-01

    We introduce the idea of distortion side information, which does not directly depend on the source but instead affects the distortion measure. We show that such distortion side information is not only useful at the encoder, but that under certain conditions, knowing it at only the encoder is as good as knowing it at both encoder and decoder, and knowing it at only the decoder is useless. Thus distortion side information is a natural complement to the signal side information studied by Wyner a...

  7. Encoding entanglement-assisted quantum stabilizer codes

    International Nuclear Information System (INIS)

    We address the problem of encoding entanglement-assisted (EA) quantum error-correcting codes (QECCs) and of the corresponding complexity. We present an iterative algorithm from which a quantum circuit composed of CNOT, H, and S gates can be derived directly with complexity O(n2) to encode the qubits being sent. Moreover, we derive the number of each gate consumed in our algorithm according to which we can design EA QECCs with low encoding complexity. Another advantage brought by our algorithm is the easiness and efficiency of programming on classical computers. (general)

  8. 基于 PLC 的船用 SCR 废气减排与综合利用%SCR emission reduction and comprehensive utilization of the ship based on PLC

    Institute of Scientific and Technical Information of China (English)

    文超

    2015-01-01

    In recent years, the country pay more and more attention to the protection of the environment, to strengthen the control of all kinds of vehicle emission, to reduce emissions of the vehicle through a number of measures. The ship is a kind of large transportation tool, which make great contributions to the transportation industry and economic development at the same time, but also cause great pollution to the natural environment. Many of the nitrogen oxides is contained in the waste gas produced by the marine diesel engine, the fact proves that these oxides has great harm to the atmosphere. The diesel engine selective catalytic reduction system is a kind of technology especially for the nitrogen oxides control technology, which can reduce more than 50% reduction of ntrogen oxides in the waste gas produced by the marine diesel engine effectively. In this paper, the SCR emission reduction and comprehensive utilization system of ship are analyzed and studied based on the PLC technology.%近年来,国家越来越重视对环境的保护,加强对各种交通工具的排放控制,通过一些措施对其进行废气减排。船舶作为一种大型的交通运输工具,在为交通运输业与经济发展做出巨大贡献的同时,也对自然环境造成了较大污染。船用柴油机会产生很多的含有氮氧化物的废气,事实证明这些氮氧化物对大气具有很大危害。而选择性催化还原系统( SCR 系统)是一种专门针对氮氧化物的控制减排技术,可以有效的减少船舶柴油机废气中50%以上的氮氧化物。本文基于 PLC 技术,对船用的 SCR 废气减排与综合利用系统进行了系统的分析与研究。

  9. Software design specification and analysis(NuFDS) approach for the safety critical software based on porgrammable logic controller(PLC)

    International Nuclear Information System (INIS)

    This paper introduces the software design specification and analysis technique for the safety-critical system based on Programmable Logic Controller (PLC). During software development phases, the design phase should perform an important role to connect between requirements phase and implementation phase as a process of translating problem requirements into software structures. In this work, the Nuclear FBD-style Design Specification and analysis (NuFDS) approach was proposed. The NuFDS approach for nuclear Instrumentation and Control (I and C) software are suggested in a straight forward manner. It consists of four major specifications as follows; Database, Software Architecture, System Behavior, and PLC Hardware Configuration. Additionally, correctness, completeness, consistency, and traceability check techniques are also suggested for the formal design analysis in NuFDS approach. In addition, for the tool supporting, we are developing NuSDS tool based on the NuFDS approach which is a tool, especially for the software design specification in nuclear fields

  10. PLC程序控制研配液压机的结构设计%Structure Design of Bedding-in Hydraulic Press Controlled by PLC Program

    Institute of Scientific and Technical Information of China (English)

    刘泽民; 付丽; 敖茜; 李慧

    2014-01-01

    The design scheme of bedding-in hydraulic press controlled by PLC was introduced. The bedding-in process,struc-ture,hydraulic system and PLC control process of the bedding-in hydraulic press were analyzed. The program significantly improves the control precision and production efficiency.%介绍了应用PLC技术的研配液压机的设计方案,对该机的研配过程、结构、液压系统和PLC控制过程进行了分析和说明。该方案的提出显著提高了控制精度和生产效率。

  11. 一种AB PLC与Profibus DP网络通信的方法%A Solution for Communication between AB PLC and Profibus DP Network

    Institute of Scientific and Technical Information of China (English)

    李学治

    2015-01-01

    Industrial automation communication network is variousness. This paper presents a solution for communication be-tween AB PLC and Profibus DP network, introduces an example that an AB PLC system communicates with Siemens S120 drives through Profibus DP network by using MVI56-PDPMV1 module.%工业自动化通信网络具有多样性的特点, wenz对AB PLC与Profibus DP网络通信的方法进行探究,用设计实例探讨AB PLC系统通过MVI56-PDPMV1通信模块与西门子S120驱动系统构建Profibus-DP通信网络的设计与编程设置。

  12. 基于PLC的智能温室监控系统研究%Intelligent Greenhouse Monitoring System based on PLC

    Institute of Scientific and Technical Information of China (English)

    吴海

    2014-01-01

    From the perspective of PLC, describes the main greenhouse constitute intelligent monitoring system, and PLC control software is designed to travel research. Research in this area can promote intelligent greenhouse control system to a higher level of development;play a role in promoting the development of our country's greenhouse cultivation.%从PLC角度出发,阐述了智能温室监控系统的主要构成,并对PLC控制软件的设计行进了研究。这方面的研究能够促使智能温室监控系统向更高的水平发展,对我国的温室种植发展起到一定的推动作用。

  13. Deformed versus undeformed cat states encoding qubit

    International Nuclear Information System (INIS)

    We study the possibility of exploiting superpositions of coherent states to encode qubits. A comparison between the use of deformed and undeformed bosonic algebra is made in connection with the amplitude damping errors

  14. Encoding and decoding of femtosecond pulses.

    Science.gov (United States)

    Weiner, A M; Heritage, J P; Salehi, J A

    1988-04-01

    We demonstrate the spreading of femtosecond optical pulses into picosecond-duration pseudonoise bursts. Spreading is accomplished by encoding pseudorandom binary phase codes onto the optical frequency spectrum. Subsequent decoding of the spectral phases restores the original pulse. We propose that frequency-domain encoding and decoding of coherent ultrashort pulses could form the basis for a rapidly reconfigurable, code-division multiple-access optical telecommunications network. PMID:19745879

  15. Unconscious relational encoding depends on hippocampus

    OpenAIRE

    Duss, Simone; Reber, Thomas P.; Hänggi, Jürgen; Schwab, Simon; Wiest, Roland; Müri, René Martin; Brugger, Peter; Gutbrod, Klemens; Henke, Katharina

    2014-01-01

    Textbooks divide between human memory systems based on consciousness. Hippocampus is thought to support only conscious encoding, while neocortex supports both conscious and unconscious encoding. We tested whether processing modes, not consciousness, divide between memory systems in three neuroimaging experiments with 11 amnesic patients (mean age = 45.55 years, standard deviation = 8.74, range = 23-60) and 11 matched healthy control subjects. Examined processing modes were single item versus ...

  16. Encoding attentional states during visuomotor adaptation

    OpenAIRE

    Im, Hee Yeon; Bédard, Patrick; Song, Joo-Hyun

    2015-01-01

    We recently showed that visuomotor adaptation acquired under attentional distraction is better recalled under a similar level of distraction compared to no distraction. This paradoxical effect suggests that attentional state (e.g., divided or undivided) is encoded as an internal context during visuomotor learning and should be reinstated for successful recall (Song & Bédard, 2015). To investigate if there is a critical temporal window for encoding attentional state in visuomotor memory, we ma...

  17. A New SAT Encoding of Earley Parsing

    OpenAIRE

    Neil, Tobias

    2015-01-01

    While the Boolean satisfiability problem (SAT) lies in NP, prodigious work in SAT solvers has allowed for its use in modeling a multitude of practical problems. Stating a problem in SAT can be cumbersome though and so the demand for SAT encodings arises, providing a means to formulate problems or parts of problems in a more intuitive environment. Several algorithms have been proposed in the past to encode context-free grammars as SAT formulae, allowing for the comprehensive construction of ma...

  18. Multi-dimensionally encoded magnetic resonance imaging

    OpenAIRE

    Lin, Fa-Hsuan

    2012-01-01

    Magnetic resonance imaging typically achieves spatial encoding by measuring the projection of a q-dimensional object over q-dimensional spatial bases created by linear spatial encoding magnetic fields (SEMs). Recently, imaging strategies using nonlinear SEMs have demonstrated potential advantages for reconstructing images with higher spatiotemporal resolution and reducing peripheral nerve stimulation. In practice, nonlinear SEMs and linear SEMs can be used jointly to further improve the image...

  19. The application of PLC to the production of cornstarch%PLC在玉米淀粉生产中的应用

    Institute of Scientific and Technical Information of China (English)

    张彦铎; 朱长山

    2001-01-01

    本文以淀粉生产中原料玉米的预处理过程为对象,应用OMRON可编程控制器(PLC)进行了控制的实现;在分析软件系统设计方法的基础上,对控制循环性生产过程的PLC系统设计方法进行了探讨.

  20. An Evaluation of the Effect of Merger and Acquisition on the Performance of Nigeria Banking Sector : Case: United Bank of Africa, PLC

    OpenAIRE

    Salawu, Kazeem

    2013-01-01

    This study was designed to study the effect of Merger and Acquisition in the Banking Industry in Nigeria with special reference to United Bank of Africa, Plc. The research work tried to reveal whether the introduction of merger and acquisi-tion can improve the banking industry profitability and provide an engine for eco-nomic growth and sustainable development. The thesis will review the background of the Nigerian Banking sector since incep-tion as well as, examine the efficacy of...

  1. 基于MODBUS协议的设备和PLC实现通信的研究%Research of communication between devices based on MODBUS protocol and PLC

    Institute of Scientific and Technical Information of China (English)

    陈铭

    2009-01-01

    简单介绍了Modbus通信协议的特点,说明可编程序控制器(PLC)在和具备Modbus协议的设备实现通信时的程序流程,详细解释程序的实现细节.对需要实现与Modbus协议的设备进行通信的设计开发人员有一定的借鉴作用.

  2. Design of Hydraulic Control System for Injection Molding Machine Based on PLC%基于PLC的注塑机液压系统的控制设计

    Institute of Scientific and Technical Information of China (English)

    李青虹; 吴龙

    2011-01-01

    The working principle about hydraulic system of injection molding machine and the working requirement are introduced,then the electrical control system including hardware and program based on the PLC is designed.The programming way of Start-Retention-Stop Electric Circuit to modular programming is employed.After drawing sequential function chart,modularized programming is achieved by using PLC programming components,therefore the program is concise,simple debugging,easy to modify,and has strong versatility,which is suitable for any type of PLC.The finding shows that:the modified electrical control system can greatly improve the production precision,working performance,maintenance and stability of injection molding machine,improve the quality and production efficiency of the products.%通过分析注塑机液压系统的工作过程,设计了基于PLC的电气控制系统,设计包括硬件和软件设计.采用先进的顺序控制设计法进行程序设计,在画出顺序功能图SFC的基础上,利用通用的PLC编程元件及起保停电路的编程方式进行模块化编程,程序具有简洁明了、调试简单、修改容易、通用性强的优点,适用于任意型号的PLC.实践表明:采用PLC改造的电气控制系统大大提高了注塑机的工作性能和稳定性,提升了注塑产品的质量和生产效率,且系统维护方便.

  3. Using PLC characteristics to improve the safe operation of the equipment%修改PLC提高设备的安全运行二例

    Institute of Scientific and Technical Information of China (English)

    张春宁; 李荣亮

    2013-01-01

    Through the improvement of PLC and control circuit of the two devices, misoperation opportunity is reduced, so the safety coefficient can be strengthened, the equipment and personnel safety can be ensured.%通过对两台设备PLC和控制线路的改进,减少误操作,使其安全运行,保障了设备与人身的安全.

  4. Toward More Efficient and More Secure Last Mile Smart Metering and Smart Lighting Communication Systems with the Use of PLC/RF Hybrid Technology

    OpenAIRE

    Piotr Kiedrowski

    2015-01-01

    This paper describes the last mile communication system solutions realized in PLC/RF hybrid technology, which is dedicated to Smart Grid communication subsystems, mainly for Smart Metering and Smart Lighting applications. The use of hybrid technology makes the system more efficient and more secure (still being of low cost, in terms of both implementation and operation). This paper proposes a novel parameter, PDR, to describe the performance of the communications in the last mile network. The ...

  5. 基于 PLC 的垂直升降式立体车库控制系统设计%Design of Control System for Vertical Garage of Lifting Type Based on PLC

    Institute of Scientific and Technical Information of China (English)

    王虎军; 马殷元

    2016-01-01

    The vertical garage of lifting type is effective programs to solve the problem of hard parking in the center of a city.This paper designed a control system of the vertical garage of lifting type and made the Siemens 300 series PLC as controller to describe the hardware components of the control system and the input and output port assign-ments, analyze how to program the main program and automatic control subroutine, and propose hardware and soft-ware methods to improve the safety and reliability of garage control system.The control system meets the functional requirements of the parking garage with high reliability.%垂直升降式立体车库是解决城市中心停车难的有效方案。设计了以西门子300系列PLC为主控制器的垂直升降式立体车库控制系统。阐述了该控制系统的硬件组成及输入输出口分配方案,编写了主程序和自动控制子程序,提出了提高车库控制系统安全与可靠性的相关设计。该控制系统能满足立体车库的功能需求并具有较高的可靠性。

  6. Development of a Measurement and Control System for a 40l/h Helium Liquefier based on Siemens PLC S7-300

    Science.gov (United States)

    Li, J.; Liu, L. Q.; Xu, X. D.; Liu, T.; Li, Q.; Hu, Z. J.; Wang, B. M.; Xiong, L. Y.; Dong, B.; Yan, T.

    A 40l/h Helium Liquefier has been commissioned by the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. A measurement and control system based on Siemens PLC S7-300 for this Helium Liquefier is developed. Proper sensors are selected, for example, three types of transmitters are adopted respectively according to detailed temperature measurement requirements. Siemens S7-300 PLC CPU315-2PN/DP operates as a master station and three sets of ET200 M DP remote expand I/O operate asslave stations. Profibus-DP field communication is used between the master station and the slave stations. The upper computer HMI(Human Machine Interface) is compiled using Siemens configuration software WinCC V7.0. The upper computer communicates with PLC by means of industrial Ethernet. A specific control logic for this Helium Liquefier is developed. The control of the suction and discharge pressures of the compressor and the control of the turbo-expanders loop are being discussed in this paper. Following the commissioning phase, the outlet temperature of the second stage turbine has reached 8.6K and the temperature before the throttle valve has reached 13.1K.

  7. Management Programme for Research Reactor Spent Nuclear Fuel Storage and Interim Storage Facilities at Nuclear Research Institute Rez Plc, Czech Republic

    International Nuclear Information System (INIS)

    LVR-15 is a research reactor that operates in NRI Rez plc, Czech Republic since 1957. From the first criticality of the reactor until 2007, more than 600 fuel assemblies have been used in the reactor core, resulting in a large quantity of spent fuel. This paper describes the management of Research Reactor Spent Nuclear Fuel (RRSNF) storage at NRI Rez plc. Characteristics of fuel types used (EK-10, IRT-2M 80% enriched and IRT-2M 36% enriched), and characteristics of interim storage facilities and infrastructure available are described. The paper emphasizes the experience gained during the preparatory works for the SNF shipment (facility and equipment modification, cask licenses) and the preparation of the SNF for transport, in particular its checking, repackaging in a hot cell, loading into the VPVR/M casks, drying, manipulation, completion of the transport documentation, etc. including its transport to the High Level Waste Storage facility before its transportation to the Russian Federation. Future Russian Federation options for the spent fuel management at NRI Rez plc, including possibility of additional shipments to the Russian Federation, or interim storage in SKODA VPVR/M cask systems at NRI Rez is also discussed. (author)

  8. Measurement and control system for cryogenic helium gas bearing turbo-expander experimental platform based on Siemens PLC S7-300

    International Nuclear Information System (INIS)

    An experimental platform for cryogenic Helium gas bearing turbo-expanders is established at the Technical Institute of Physics and Chemistry, Chinese Academy of Sciences. This turbo-expander experimental platform is designed for performance testing and experimental research on Helium turbo-expanders with different sizes from the liquid hydrogen temperature to the room temperature region. A measurement and control system based on Siemens PLC S7-300 for this turbo-expander experimental platform is developed. Proper sensors are selected to measure such parameters as temperature, pressure, rotation speed and air flow rate. All the collected data to be processed are transformed and transmitted to S7-300 CPU. Siemens S7-300 series PLC CPU315-2PN/DP is as master station and two sets of ET200M DP remote expand I/O is as slave station. Profibus-DP field communication is established between master station and slave stations. The upper computer Human Machine Interface (HMI) is compiled using Siemens configuration software WinCC V6.2. The upper computer communicates with PLC by means of industrial Ethernet. Centralized monitoring and distributed control is achieved. Experimental results show that this measurement and control system has fulfilled the test requirement for the turbo-expander experimental platform

  9. Design of a PWM Speed - governing System for DC Motor Based on PLC%基于PLC的直流电机PWM调速系统设计

    Institute of Scientific and Technical Information of China (English)

    何新霞; 邢瑞军

    2011-01-01

    设计了基于SIMATIC S7-200 PLC直流电机PWM调速系统.主电路采用双极式可逆PWM变换器,选用集成驱动器M57962L,通过设计其驱动外围电路,实现了对IGBT的导通和关断控制.采用硬件延时电路,防止上、下桥臂直通事故的发生.利用PLC的高速计数功能实现了对电机转速的测量,设计开发了基于PLC的PI控制程序及PWM信号产生程序.从而实现了直流电机的PWM调速,并实现了转速的无静差调节.%The paper presents the design of PWM speed - governing system for DC motor based on S7 - 200 - PLC. The double - polar reversible PWM converter is used and integrated drive electronics M57962 is selected in main circuit. The conduction and switch control of IGBT is realized by designing the peripheral driving circuitry. The delayed circuit is adopted to prevent the shoot -through phenomenon between upper and lower bridge arm in a PWM. Programs for PLC PI control and for PWM signal generation are developed to implement PWM speed- governing for DC motor and floating control on speed.

  10. Z3040型摇臂钻床的PLC控制%The PLC Control of Z3040 Type Radial Drilling Machine

    Institute of Scientific and Technical Information of China (English)

    嵇海旭; 梁秀娟

    2015-01-01

    Using PLC to control Z3040 can make the complex wiring is simplified greatly, reduce the failure rate. The paper introduces the use of PLC on the traditional Z3040 type radial drilling machine control, completes the design of wiring diagram of input and output devices,and designs the complete ladder chart. The paper points out the processing on the external input device normally closed contact, power failure delay ,and the security processing reversing control ,etc. It can provide reference to the PLC cotrol of other machine tools.%采用PLC对Z3040进行控制,可以使复杂接线大为简化,降低故障率。介绍了用PLC对传统Z3040型摇臂钻床的控制,完成了输入输出设备的接线图和控制梯形图的设计工作,指出了外部输入设备常闭触点在梯形图中的处理,断电延时的处理,正反转控制的安全处理等,对其他机床设备的PLC控制提供借鉴作用。

  11. Design of Electrical Training Equipment Control System Based on PLC%PLC电工实训装置控制系统设计

    Institute of Scientific and Technical Information of China (English)

    周飞; 张英琦

    2013-01-01

    以矿井提升绞车为模型,设计了一个基于PLC的电工实训装置,从功能上讲,主要由提升绞车模型、PLC电气控制系统、变频器变频调速系统、触摸屏监控系统组成。给出了系统方案,设计了PLC控制系统、信号检测电路和变频器控制电路。该实训装置突出“工矿”特色,既包括各种电气控制技术的基础实践训练,又包括多种电工技术的综合性实践训练。%Taking the mine hoist as model, electrical training equipment based on PLC is designed in the paper. From the func-tional point of view, the equipment is mainly composed of mine hoist model, PLC control system, inverter frequency control sys-tem and touch screen monitoring systems. The system scheme is proposed. The PLC control system, signal detection circuit and the inverter control system is designed. The training device outstanding industrial and mineral characteristic, including both vari-ous electric control technology foundation practice training, and variety of electrical technologies comprehensive practical train-ing.

  12. Involvement of MAPKs and PLC Pathways in Modulation of Pacemaking Activity by So-Cheong-Ryong-Tang in Interstitial Cells of Cajal from Murine Small Intestine

    Directory of Open Access Journals (Sweden)

    Min Woo Hwang

    2013-01-01

    Full Text Available Purpose. Interstitial cells of Cajal (ICCs are the pacemaker cells that generate slow waves in the gastrointestinal (GI tract. We have aimed to investigate the effects of Socheongryong-Tang (SCRT in ICCs from mouse’s small intestine. Methods. The whole-cell patch-clamp configuration was used to record membrane potentials from cultured ICCs. Intracellular Ca2+ ([Ca2+]i increase was studied in cultured ICCs using fura-2 AM. Results. ICCs generated pacemaker potentials in mouse’s small intestine. SCRT produced membrane depolarization in current clamp mode. Y25130 (5-HT3 receptor antagonist and RS39604 (5-HT4 receptor antagonist blocked SCRT-induced membrane depolarizations, whereas SB269970 (5-HT7 receptor antagonist did not. When GDP-β-S (1 mM was in the pipette solution, SCRT did not induce the membrane depolarizations. [Ca2+]i analysis showed that SCRT increased [Ca2+]i. In the presence of PD98059 (p42/44 MAPK inhibitor, SCRT did not produce membrane depolarizations. In addition, SB203580 (p38 MAPK inhibitor and JNK inhibitors blocked the depolarizations by SCRT in pacemaker potentials. Furthermore, the membrane depolarizations by SCRT were not inhibited by U-73122, an active phospholipase C (PLC inhibitor, but by U-73343, an inactive PLC inhibitor. Conclusion. These results suggest that SCRT might affect GI motility by the modulation of pacemaker activity through MAPKs and PLC pathways in the ICCs.

  13. An encyclopedia of mouse DNA elements (Mouse ENCODE)

    OpenAIRE

    Stamatoyannopoulos, John A; Guig?? Serra, Roderic; Djebali, Sarah; Lagarde, Julien; Adams, Leslie B.

    2012-01-01

    To complement the human Encyclopedia of DNA Elements (ENCODE) project and to enable a broad range of mouse genomics efforts, the Mouse ENCODE Consortium is applying the same experimental pipelines developed for human ENCODE to annotate the mouse genome.

  14. Multichannel Compressive Sensing MRI Using Noiselet Encoding

    Science.gov (United States)

    Pawar, Kamlesh; Egan, Gary; Zhang, Jingxin

    2015-01-01

    The incoherence between measurement and sparsifying transform matrices and the restricted isometry property (RIP) of measurement matrix are two of the key factors in determining the performance of compressive sensing (CS). In CS-MRI, the randomly under-sampled Fourier matrix is used as the measurement matrix and the wavelet transform is usually used as sparsifying transform matrix. However, the incoherence between the randomly under-sampled Fourier matrix and the wavelet matrix is not optimal, which can deteriorate the performance of CS-MRI. Using the mathematical result that noiselets are maximally incoherent with wavelets, this paper introduces the noiselet unitary bases as the measurement matrix to improve the incoherence and RIP in CS-MRI. Based on an empirical RIP analysis that compares the multichannel noiselet and multichannel Fourier measurement matrices in CS-MRI, we propose a multichannel compressive sensing (MCS) framework to take the advantage of multichannel data acquisition used in MRI scanners. Simulations are presented in the MCS framework to compare the performance of noiselet encoding reconstructions and Fourier encoding reconstructions at different acceleration factors. The comparisons indicate that multichannel noiselet measurement matrix has better RIP than that of its Fourier counterpart, and that noiselet encoded MCS-MRI outperforms Fourier encoded MCS-MRI in preserving image resolution and can achieve higher acceleration factors. To demonstrate the feasibility of the proposed noiselet encoding scheme, a pulse sequences with tailored spatially selective RF excitation pulses was designed and implemented on a 3T scanner to acquire the data in the noiselet domain from a phantom and a human brain. The results indicate that noislet encoding preserves image resolution better than Fouirer encoding. PMID:25965548

  15. Multichannel compressive sensing MRI using noiselet encoding.

    Directory of Open Access Journals (Sweden)

    Kamlesh Pawar

    Full Text Available The incoherence between measurement and sparsifying transform matrices and the restricted isometry property (RIP of measurement matrix are two of the key factors in determining the performance of compressive sensing (CS. In CS-MRI, the randomly under-sampled Fourier matrix is used as the measurement matrix and the wavelet transform is usually used as sparsifying transform matrix. However, the incoherence between the randomly under-sampled Fourier matrix and the wavelet matrix is not optimal, which can deteriorate the performance of CS-MRI. Using the mathematical result that noiselets are maximally incoherent with wavelets, this paper introduces the noiselet unitary bases as the measurement matrix to improve the incoherence and RIP in CS-MRI. Based on an empirical RIP analysis that compares the multichannel noiselet and multichannel Fourier measurement matrices in CS-MRI, we propose a multichannel compressive sensing (MCS framework to take the advantage of multichannel data acquisition used in MRI scanners. Simulations are presented in the MCS framework to compare the performance of noiselet encoding reconstructions and Fourier encoding reconstructions at different acceleration factors. The comparisons indicate that multichannel noiselet measurement matrix has better RIP than that of its Fourier counterpart, and that noiselet encoded MCS-MRI outperforms Fourier encoded MCS-MRI in preserving image resolution and can achieve higher acceleration factors. To demonstrate the feasibility of the proposed noiselet encoding scheme, a pulse sequences with tailored spatially selective RF excitation pulses was designed and implemented on a 3T scanner to acquire the data in the noiselet domain from a phantom and a human brain. The results indicate that noislet encoding preserves image resolution better than Fouirer encoding.

  16. ESPRESSO instrument control electronics: a PLC based distributed layout for a second generation instrument at ESO VLT

    Science.gov (United States)

    Baldini, V.; Cirami, R.; Coretti, I.; Cristiani, S.; Di Marcantonio, P.; Mannetta, M.; Santin, P.; Mégevand, D.; Zerbi, F.

    2014-07-01

    ESPRESSO is an ultra-stable fiber-fed spectrograph designed to combine incoherently the light coming from up to 4 Unit Telescopes of the ESO VLT. From the Nasmyth focus of each telescope the light, through an optical path, is fed by the Coudé Train subsystems to the Front End Unit placed in the Combined Coudé Laboratory. The Front End is composed by one arm for each telescope and its task is to convey the incoming light, after a calibration process, into the spectrograph fibers. To perform these operations a large number of functions are foreseen, like motorized stages, lamps, digital and analog sensors that, coupled with dedicated Technical CCDs (two per arms), allow to stabilize the incoming beam up to the level needed to exploit the ESPRESSO scientific requirements. The Instrument Control Electronics goal is to properly control all the functions in the Combined Coudé Laboratory and the spectrograph itself. It is fully based on a distributed PLC architecture, abandoning in this way the VME-based technology previously adopted for the ESO VLT instruments. In this paper we will describe the ESPRESSO Instrument Control Electronics architecture, focusing on the distributed layout and its interfaces with the other ESPRESSO subsystems.

  17. Design of Embedded PLC System and Its Implementation%嵌入式PLC系统的设计与实现

    Institute of Scientific and Technical Information of China (English)

    严奎

    2012-01-01

    An embedded PLC system was designed and implemented based on Linux operation system and ARM embedded platform, and hardware composition and software design methods including development system and running system of the system were introduced in details. The system was tested on platform of electrochemistry working station of an university. The test result showed the system is simple in operation and flexible in control, but still needs improving electromagnetic compatibility and robustness.%基于Linux操作系统和ARM嵌入式平台设计并实现了一种嵌入式PLC系统,详细介绍了该系统的硬件组成及开发系统、运行系统的软件设计方法.该系统已在某高校电化学工作站平台上进行了实用性测试,结果表明,该系统操作步骤简单易掌握,控制较为灵活,但仍需进一步改进其电磁兼容性和健壮性.

  18. PLC Control and Design of the Universal Sawing Machine%万向锯床的PLC控制及设计

    Institute of Scientific and Technical Information of China (English)

    刘桂兰

    2015-01-01

    the universal Sawing machine solved the single direction' s shortcomings of general cutting machine. this paper introduces designing requirements of universal Sawing machine, motor driving circuit and the principle of hydraulic transmission, using FX2N-48MR series Mitsubishi PLC overall controls the universal machine. According to the control requirements, determines the specific input/output devices, and I/O distribution, designs hardware connection and software ladder diagram, finally carries on the scene debugging.%万向锯床解决了通用锯床锯切单一方向的缺点,介绍了万向锯床的设计工艺要求,液压传动原理,电机驱动电路和三菱FX2N-48MR系列PLC对万向锯床的总体控制方案.根据控制要求确定输入/输出设备,并进行I/O口分配,硬件接线和软件梯形图的设计,最后进行现场调试.

  19. Application of Programmable Logic Controller (PLC) in Control System for Inner and Outer Welding Processes of Irradiation Chamber

    International Nuclear Information System (INIS)

    Inner and outer welding processes of irradiation chamber require reliable human resources so that the welding results will meet the standardized requirements for irradiation with nuclear reactor. The control system of the inner and outer welding processes of the irradiation chamber was made due to limited human resources who are able to weld the irradiation chamber perfectly. The irradiation chamber’s welding is expected to be done automatically by using the system control. The system is created by using Programmable Logic Controller (PLC) which function is to automatically organize the welding processes through the codes attached there in. The system comprises of a control unit which has been tested in a simulation, and then attached in a welding machine unit, and subsequently tested for welding the irradiation chamber. The result of the welding is also tested for its leak using bubble test. As all procedures are well followed in the welding processes, the control system is found to work properly. The welding results which are not optimum are mainly due to imperfect irradiation tubes, such as the lack of symmetry in the tube’s geometry, unclean surface, as well as the condition of the unprecise mechanical part of the tubes. (author)

  20. Noise level and MPEG-2 encoder statistics

    Science.gov (United States)

    Lee, Jungwoo

    1997-01-01

    Most software in the movie and broadcasting industries are still in analog film or tape format, which typically contains random noise that originated from film, CCD camera, and tape recording. The performance of the MPEG-2 encoder may be significantly degraded by the noise. It is also affected by the scene type that includes spatial and temporal activity. The statistical property of noise originating from camera and tape player is analyzed and the models for the two types of noise are developed. The relationship between the noise, the scene type, and encoder statistics of a number of MPEG-2 parameters such as motion vector magnitude, prediction error, and quant scale are discussed. This analysis is intended to be a tool for designing robust MPEG encoding algorithms such as preprocessing and rate control.

  1. A Metric Encoding for Bounded Model Checking

    Science.gov (United States)

    Pradella, Matteo; Morzenti, Angelo; San Pietro, Pierluigi

    In Bounded Model Checking, both the system model and the checked property are translated into a Boolean formula to be analyzed by a SAT-solver. We introduce a new encoding technique which is particularly optimized for managing quantitative future and past metric temporal operators, typically found in properties of hard real time systems. The encoding is simple and intuitive in principle, but it is made more complex by the presence, typical of the Bounded Model Checking technique, of backward and forward loops used to represent an ultimately periodic infinite domain by a finite structure. We report and comment on the new encoding technique and on an extensive set of experiments carried out to assess its feasibility and effectiveness.

  2. Template based low data rate speech encoder

    Science.gov (United States)

    Fransen, Lawrence

    1993-09-01

    The 2400-b/s linear predictive coder (LPC) is currently being widely deployed to support tactical voice communication over narrowband channels. However, there is a need for lower-data-rate voice encoders for special applications: improved performance in high bit-error conditions, low-probability-of-intercept (LPI) voice communication, and narrowband integrated voice/data systems. An 800-b/s voice encoding algorithm is presented which is an extension of the 2400-b/s LPC. To construct template tables, speech samples of 420 speakers uttering 8 sentences each were excerpted from the Texas Instrument - Massachusetts Institute of Technology (TIMIT) Acoustic-Phonetic Speech Data Base. Speech intelligibility of the 800-b/s voice encoding algorithm measured by the diagnostic rhyme test (DRT) is 91.5 for three male speakers. This score compares favorably with the 2400-b/s LPC of a few years ago.

  3. Incremental phonological encoding during unscripted sentence production

    Directory of Open Access Journals (Sweden)

    Florian T Jaeger

    2012-11-01

    Full Text Available We investigate phonological encoding during unscripted sentence production, focusing on the effect of phonological overlap on phonological encoding. Previous work on this question has almost exclusively employed isolated word production or highly scripted multiword production. These studies have led to conflicting results: some studies found that phonological overlap between two words facilitates phonological encoding, while others found inhibitory effects. One worry with many of these paradigms is that they involve processes that are not typical to everyday language use, which calls into question to what extent their findings speak to the architectures and mechanisms underlying language production. We present a paradigm to investigate the consequences of phonological overlap between words in a sentence while leaving speakers much of the lexical and structural choices typical in everyday language use. Adult native speakers of English described events in short video clips. We annotated the presence of disfluencies and the speech rate at various points throughout the sentence, as well as the constituent order. We find that phonological overlap has an inhibitory effect on phonological encoding. Specifically, if adjacent content words share their phonological onset (e.g., hand the hammer, they are preceded by production difficulty, as reflected in fluency and speech rate. We also find that this production difficulty affects speakers’ constituent order preferences during grammatical encoding. We discuss our results and previous works to isolate the properties of other paradigms that resulted in facilitatory or inhibitory results. The data from our paradigm also speak to questions about the scope of phonological planning in unscripted speech and as to whether phonological and grammatical encoding interact.

  4. Optical encoder based on a nondiffractive beam

    International Nuclear Information System (INIS)

    Optical encoders are used in industrial and laboratory motion equipment to measure rotations and linear displacements. We introduce a design of an optical encoder based on a nondiffractive beam. We expect that the invariant profile and radial symmetry of the nondiffractive beam provide the design with remarkable tolerance to mechanical perturbations. We experimentally demonstrate that the proposed design generates a suitable output sinusoidal signal with low harmonic distortion. Moreover, we present a numerical model of the system based on the angular spectrum approximation whose predictions are in excellent agreement with the experimental results

  5. Flow imaging with phase velocity encoding

    International Nuclear Information System (INIS)

    The authors studied blood flow quantitation by MR phase velocity encoding (PVE) under steady-flow conditions on in vitro models. Different flow conditions that might cause signal loss were studied: high velocities, velocity gradients, and turbulence. Results showed the failure of the PVE technique in the presence of highly turbulent flows. On the other hand, PVE could measure accurately flows for which radial and circumferential turbulence intensity did not exceed 8 cm/sec when a 1-mm scan resolution is used. The authors concluded that turbulence disturbed the spatial encoding of proton position and therefore induced signal loss

  6. Encoding and Decoding Procedures for Arrangements

    Directory of Open Access Journals (Sweden)

    Alexander A. Babaev

    2012-05-01

    Full Text Available This article discusses an algorithm based on the encoding procedure for representing a set of arrangement elements as a single number. Also the author provides the procedure for the inverse transformation of the code into arrangement elements. In addition the Article includes recommendations on the use of the above procedures in combinatorial algorithms of optimization.

  7. Encoded Archival Description as a Halfway Technology

    Science.gov (United States)

    Dow, Elizabeth H.

    2009-01-01

    In the mid 1990s, Encoded Archival Description (EAD) appeared as a revolutionary technology for publishing archival finding aids on the Web. The author explores whether or not, given the advent of Web 2.0, the archival community should abandon EAD and look for something to replace it. (Contains 18 notes.)

  8. Encoding attentional states during visuomotor adaptation.

    Science.gov (United States)

    Im, Hee Yeon; Bédard, Patrick; Song, Joo-Hyun

    2015-01-01

    We recently showed that visuomotor adaptation acquired under attentional distraction is better recalled under a similar level of distraction compared to no distraction. This paradoxical effect suggests that attentional state (e.g., divided or undivided) is encoded as an internal context during visuomotor learning and should be reinstated for successful recall (Song & Bédard, 2015). To investigate if there is a critical temporal window for encoding attentional state in visuomotor memory, we manipulated whether participants performed the secondary attention-demanding task concurrently in the early or late phase of visuomotor learning. Recall performance was enhanced when the attentional states between recall and the early phase of visuomotor learning were consistent. However, it reverted to untrained levels when tested under the attentional state of the late-phase learning. This suggests that attentional state is primarily encoded during the early phase of learning before motor errors decrease and reach an asymptote. Furthermore, we demonstrate that when divided and undivided attentional states were mixed during visuomotor adaptation, only divided attention was encoded as an internal cue for memory retrieval. Therefore, a single attentional state appears to be primarily integrated with visuomotor memory while motor error reduction is in progress during learning. PMID:26114683

  9. Encoding and Retrieval During Bimanual Rhythmic Coordination

    Science.gov (United States)

    Shockley, Kevin; Turvey, Michael T.

    2005-01-01

    In 2 experiments, bimanual 1:1 rhythmic coordination was performed concurrently with encoding or retrieval of word lists. Effects of divided attention (DA) on coordination were indexed by changes in mean relative phase and recurrence measures of shared activity between the 2 limbs. Effects of DA on memory were indexed by deficits in recall…

  10. An Encoder/Decoder Scheme of OCDMA Based on Waveguide

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    A new encoder/decoder scheme of OCDMA based on waveguide isproposed in this paper. The principle as well as the structure of waveguide encoder/decoder is given. It can be seen that all-optical OCDMA encoder/decoder can be realized by the proposed scheme of the waveguide encoder/decoder. It can also make the OCDMA encoder/decoder integrated easily and the access controlled easily. The system based on this scheme can work under the entirely asynchronous condition.

  11. 基于PLC的钢管环缝焊接性能研究%Performance of circumferential welding for steel tube based on PLC

    Institute of Scientific and Technical Information of China (English)

    陈怀忠; 许雪贵; 黄芳

    2014-01-01

    PLC technique was applied for controlling the CO2 gas shielded welding process of circumferential welding for 20MnCr steel tube. The corrosion resistance,thermal fatigue resistance,tensile and impact properties were tested and analyzed. The results show that compared with the common control method,the PLC control technique can obtain better corrosion resistance, thermal fatigue resistance,tensile and impact properties of welding joint. After neutral salt-spray corrosion for 240 h,the mass loss rate of circumferential welding joint by PLC technique decreases by 65%,tensile strength at 20℃increases by 15%,and impact energy at 20℃increases by 26%.%在20MnCr钢管环缝CO2气体保护焊控制过程中引入PLC技术,进行焊接接头耐腐蚀性能、抗热疲劳性能、拉伸和冲击性能的测试与对比分析。结果表明:与传统控制技术相比,采用PLC技术获得的焊接接头具有更优的耐腐蚀性能、抗热疲劳性能、拉伸和冲击性能;经过240 h中性盐雾腐蚀后质量损失率降低了65%;20℃抗拉强度增加15%、冲击吸收功增加26%。

  12. DESIGN OF PLC-BASED CONTROL SYSTEM FOR ROTATIONAL MOULDING MACHINE%基于PLC的滚塑机控制系统设计

    Institute of Scientific and Technical Information of China (English)

    孟宪辉; 李莉

    2007-01-01

    分析了滚塑机的控制过程及其控制要求,进行了包括控制方式、可靠性与安全性在内的系统设计,开发了基于可编程控制器(PLC)的控制系统.该系统安装在三种不同滚塑工艺的滚塑机上,已经投产且运行情况良好.

  13. PLC在自动售货机控制系统中的应用%The application of PLC in vending machine control system

    Institute of Scientific and Technical Information of China (English)

    董淑冷; 茅红伟

    2007-01-01

    首先介绍了自动售货机的结构组成,简述了利用可编程序控制器(PLC)控制的自动售货机控制系统的控制原理,最后给出了该控制系统的软硬件设计,并编写了系统程序总框图和一个具体的梯形图,经实践验证该控制系统是可行的.

  14. PLC在污水泵站控制系统中的应用%The application of PLC in vending machine control system

    Institute of Scientific and Technical Information of China (English)

    董淑冷; 茅红伟; 唐晓俊

    2008-01-01

    本文介绍了利用可编程序控制器(PLC)对污水泵站控制系统进行自动化改造,给出了该控制系统的控制要求、软硬件设计,简述了监控系统并给出了监控界面,经实践表明:该系统设计符合要求,系统运行安全、可靠.

  15. New Concept of PLC Modems: Multi-Carrier System for Frequency Selective Slow-Fading Channels Based on Layered SCCC Turbocodes

    OpenAIRE

    J. Zavrtalek; Kekrt, D.; Hrad, J.

    2015-01-01

    The article introduces a novel concept of a PLC modem as a complement to the existing G3 and PRIME standards for communications using medium- or high-voltage overhead or cable lines. The proposed concept is based on the fact that the levels of impulse noise and frequency selectivity are lower on high-voltage lines than on low-voltage ones. Also, the demands for “cost-effective” circuitry design are not so crucial as in the case of modems for low-voltage level. In contract to these positive co...

  16. 蓝猪耳胚珠磷脂酶C基因TfPLC1启动子的克隆、序列分析及植物表达载体的构建%Clone, Sequence Analysis and Plant Expression Vector Construction of Ovule Phospholipase C Gene TfPLC1 Promoter from Torenia fournieri

    Institute of Scientific and Technical Information of China (English)

    罗滟苏; 宋梅芳; 韩玉珍

    2007-01-01

    应用衔接头PCR技术,以蓝猪耳(Torenia fournieri)全基因组DNA分别经Dra Ⅰ、EcoR Ⅴ、Pvu Ⅱ和Sma Ⅰ消化后与衔接头连接产物为模板,用衔接头引物和TfPLC1基因的特异引物经过多轮的巢式PCR,先后克隆到2个798和813 bp的TfPLC1基因上游序列;经测序、blast比较分析和拼接得到一个蓝猪耳TfPLC1基因的启动子序列,共1 432 bp.序列分析表明它含有类似于TATAbox和CAATbox的元件,在其远端上游区域还有多个AT富含区,而且还含有多个胚乳特异表达启动子的元件.将该启动子全序列和5'端缺失的700 bp序列与PBI121分别构建了植物表达载体PPP1326和PPP700,用于转化蓝猪耳,以验证该启动子的功能.

  17. Temporal information encoding in dynamic memristive devices

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Wen; Chen, Lin; Du, Chao; Lu, Wei D., E-mail: wluee@eecs.umich.edu [Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, Michigan 48109 (United States)

    2015-11-09

    We show temporal and frequency information can be effectively encoded in memristive devices with inherent short-term dynamics. Ag/Ag{sub 2}S/Pd based memristive devices with low programming voltage (∼100 mV) were fabricated and tested. At weak programming conditions, the devices exhibit inherent decay due to spontaneous diffusion of the Ag atoms. When the devices were subjected to pulse train inputs emulating different spiking patterns, the switching probability distribution function diverges from the standard Poisson distribution and evolves according to the input pattern. The experimentally observed switching probability distributions and the associated cumulative probability functions can be well-explained using a model accounting for the short-term decay effects. Such devices offer an intriguing opportunity to directly encode neural signals for neural information storage and analysis.

  18. Asymmetric synthesis using chiral-encoded metal.

    Science.gov (United States)

    Yutthalekha, Thittaya; Wattanakit, Chularat; Lapeyre, Veronique; Nokbin, Somkiat; Warakulwit, Chompunuch; Limtrakul, Jumras; Kuhn, Alexander

    2016-01-01

    The synthesis of chiral compounds is of crucial importance in many areas of society and science, including medicine, biology, chemistry, biotechnology and agriculture. Thus, there is a fundamental interest in developing new approaches for the selective production of enantiomers. Here we report the use of mesoporous metal structures with encoded geometric chiral information for inducing asymmetry in the electrochemical synthesis of mandelic acid as a model molecule. The chiral-encoded mesoporous metal, obtained by the electrochemical reduction of platinum salts in the presence of a liquid crystal phase and the chiral template molecule, perfectly retains the chiral information after removal of the template. Starting from a prochiral compound we demonstrate enantiomeric excess of the (R)-enantiomer when using (R)-imprinted electrodes and vice versa for the (S)-imprinted ones. Moreover, changing the amount of chiral cavities in the material allows tuning the enantioselectivity. PMID:27562028

  19. Multichannel Compressive Sensing MRI Using Noiselet Encoding

    CERN Document Server

    Pawar, Kamlesh; Zhang, Jingxin

    2014-01-01

    The incoherence between measurement and sparsifying transform matrices and the restricted isometry property (RIP) of measurement matrix are two of the key factors in determining the performance of compressive sensing (CS). In CS-MRI, the randomly under-sampled Fourier matrix is used as the measurement matrix and the wavelet transform is usually used as sparsifying transform matrix. However, the incoherence between the randomly under-sampled Fourier matrix and the wavelet matrix is not optimal, which can deteriorate the performance of CS-MRI. Using the mathematical result that noiselets are maximally incoherent with wavelets, this paper introduces the noiselet unitary bases as the measurement matrix to improve the incoherence and RIP in CS-MRI, and presents a method to design the pulse sequence for the noiselet encoding. This novel encoding scheme is combined with the multichannel compressive sensing (MCS) framework to take the advantage of multichannel data acquisition used in MRI scanners. An empirical RIP a...

  20. Robust macroscopic entanglement without complex encodings

    CERN Document Server

    Chaves, Rafael; Acín, Antonio

    2011-01-01

    One of the main challenges for the experimental manipulation and storage of macroscopic entanglement is its fragility under noise. We present a simple recipe for the systematic enhancement of the resistance of multipartite entanglement against any local noise with a privileged direction in the Bloch sphere. For the case of exact local dephasing along any given basis, and for all noise strengths, our prescription grants full robustness: even states whose entanglement decays exponentially with the number of parts are mapped to states whose entanglement is constant. In contrast to previous techniques resorting to complex logical-qubit encodings, such enhancement is attained simply by performing local unitary rotations before the noise acts. The scheme is therefore highly experimentally-friendly, as it brings no overhead of extra physical qubits to encode logical ones. In addition, we show that, apart from entanglement, the resilience of the states as resources for useful practical tasks such as metrology and non...

  1. Phase modulation pseudocolor encoding ghost imaging

    Institute of Scientific and Technical Information of China (English)

    段德洋; 张路; 杜少将; 夏云杰

    2015-01-01

    We present a ghost imaging scheme that can obtain a good pseudocolor image of black-and-white objects. The essential idea is to use the multi-wavelength thermal light source and the phase modulation pseudocolor encoding technique, which overcomes the disadvantages of other methods involved the spatial filtering. Therefore, the pseudocolor ghost image achieved by this imaging scheme is better than that obtained by other methods in brightness, color, and signal-to-noise ratio.

  2. Context Is Routinely Encoded During Emotion Perception

    OpenAIRE

    Barrett, Lisa Feldman; Kensinger, Elizabeth A.

    2010-01-01

    In the present study, we investigated whether context is routinely encoded during emotion perception. For the first time, we show that people remember the context more often when asked to label an emotion in a facial expression than when asked to judge the expression's simple affective significance (which can be done on the basis of the structural features of the face alone). Our findings are consistent with an emerging literature showing that facial muscle actions (i.e., structural features ...

  3. Encoding a logical qubit into physical qubits

    OpenAIRE

    Zeng, B; Zhou, D. L.; Xu, Z.; Sun, C. P.; You, L.

    2004-01-01

    We propose two protocols to encode a logical qubit into physical qubits relying on common types of qubit-qubit interactions in as simple forms as possible. We comment on its experimental implementation in several quantum computing architectures, e.g. with trapped atomic ion qubits, atomic qubits inside a high Q optical cavity, solid state Josephson junction qubits, and Bose-Einstein condensed atoms.

  4. Implementation of communication between S7-300 PLC and single chip microproccesor based on MPI protocol%基于MPI协议的S7—300 PLC与单片机通信的实现

    Institute of Scientific and Technical Information of China (English)

    梁丁; 欧阳波

    2011-01-01

    The MPI agreement configuration between PLC may article introduces realization of the data communication application Simens S7-300 PLC and the single chip microproccesor. be primarily/main agreement-or main/from the agreement. This example and the main establishment based on MPI agreement%MPI协议在PLC之间可组态为主/主协议或主/从协议。介绍了基于MPI协议的西门子S7—300PLC与单片机实现数据通信的应用实例及其主要设置。

  5. The mortality and cancer morbidity experience of employees at the Chapelcross plant of British Nuclear Fuels plc, 1955-95

    International Nuclear Information System (INIS)

    The results presented here are from the follow-up of the cohort of workers ever employed at the Chapelcross site of British Nuclear Fuels plc (BNFL) between 1955 and 1995. The study cohort consists of 2628 workers, 2249 of whom were male, who were first employed at the plant before 1 January 1996, and who have 63 967 person-years of follow-up. The mean follow-up period is 24.3 years. The 2209 members of the cohort (84%) classified as radiation workers accumulated 185.1 person-sieverts of external radiation; their median cumulative dose was 39.1 mSv, and 95% of their cumulative doses were less than 339.3 mSv. The Chapelcross workers show the usual 'healthy worker' effect. To the end of 1995, there were 528 deaths among the total cohort (20%), including 449 (20%) amongst the radiation workers. When the dose was unlagged, a statistically significant association was noted between cancer registrations of the buccal cavity and pharynx and dose, based on five cases. When the dose was lagged by 10 years, a statistically significant excess relative risk was noted between all cancer morbidity and dose, 1.80 Sv-1 (0.03 to 4.45), based on 162 cases. This result is driven by the non-significant, but high excess relative risk estimates from the 12 prostatic cancer registrations. A statistically significant association is noted between the eight deaths amongst radiation workers who had prostatic cancer as the underlying cause of mortality and cumulative external radiation dose when the dose was lagged by 0, 2 and 10 years. The association is unlikely to be causal. The finding has little biological plausibility as the strength of the association weakened as the dose lagging increased; it was strongest when the dose was unlagged and disappeared when the dose was lagged by 20 years. None of the workers who was registered for or died from prostatic cancer had ever been monitored for exposure to tritium or to 51Cr, 59Fe, 60Co or 65Zn. There is no evidence to date amongst the

  6. Shipment of Spent Nuclear Fuel from the Nuclear Research Institute Rez plc, Czech Republic to the Russian Federation for Reprocessing

    International Nuclear Information System (INIS)

    After 50 years of operation of the research reactor operated by the Nuclear Research Institute Rez plc (NRI), a large amount of spent nuclear fuel (SNF) of Russian origin has been accumulated. In 2005, NRI joined the Russian Research Reactor Fuel Return (RRRFR) program under the US-Russian Global Threat Reduction Initiative (GTRI) and started the process of SNF shipment from the LVR-15 research reactor back to the Russian Federation (RF). SNF shipment from NRI to the Russian Federation represents a very complex and complicated scope of work, technically, legally and contractually. The SNF shipment has been realized under several specific conditions: 1. High capacity SKODA VPVR/M casks were used for transportation for the first time, which enabled the shipment of both high and low enriched SNF (about 550 fuel assemblies) in one shipment, resulting in substantially reduced risk. 2. For the first time, high enriched uranium SNF from a research reactor has been sent to the RF from a European Union country under the appropriate intergovernmental agreements, legal regulations and conditions. 3. Combined road (ADR) and railway (RID) transport of the dangerous material was used, with several re-loadings of goods. The following topics are described in the paper: - The types of SNF from the LVR-15 reactor and the quantity of particular types. - Experience gained during preparatory work for the SNF shipment. - Experience gained during preparation and fulfillment of the frame contract between the US DOE and NRI, the contract between IAEA, SKODA JS and NRI to supply a transport and packaging system, and negotiations and contracts between NRI and respective subcontracting organizations in the Czech Republic, Russian Federation and transit countries Slovakia and Ukraine, to ensure the goal and schedule of the project were met. - Experience gained during the legislative and legal process to obtain all licenses, permissions and agreements in the Czech Republic, transit countries

  7. Listeria monocytogenes Mutants Carrying Newcastle Disease Virus F Gene Fused to its actA and plcB: In vitro Expression and Immunogenicity in Chickens

    Institute of Scientific and Technical Information of China (English)

    Lingli JIANG; Chunlin KE; Jingjing XU; Jianshun CHEN; Xueyan CHEN; Ning CHEN; Jiangbing SHUAI; Weihuan FANG

    2007-01-01

    Recombinant Listeria monocytogenes mutants carrying Newcastle disease virus (NDV) fusion protein gene F were constructed by homologous recombination. NDV F or its truncated fragment Fa was used as the model heterologous gene to be integrated into actA or plcB downstream of their signal sequences.Correct orientation of the inserted genes was verified by polymerase chain reaction amplification of F or Fa.The inserted F and Fa were expressed in the two recombinants Lm-ΔactA-F and Lm-ΔplcB-Fa as shown by sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot. Both recombinants exhibited reduced virulence to embryonated eggs and mice by about 1.5-2.5 logs as compared with the parent wild strain 10403S. They were also less invasive than strain 10403S (P<0.05). Chickens receiving the recombinant strains orally or intraperitoneally were partially protected from virulent NDV challenge possibly due to enhancement of non-specific immunity because the antibody titers against the homologous virus strain or the recombinant truncated fusion protein were marginal. Further research is needed in other animal models to see if the low antibody response results from insufficient expression of the heterologous genes as a result of failure of L. monocytogenes or its recombinants to persist or replicate in chickens.

  8. Changes in Teachers' Beliefs about Reformed Science Teaching and Learning, and Their Inquiry-Based Instructional Practices Following a Year-Long RET-PLC Professional Development Program

    Science.gov (United States)

    Miranda, R.

    2014-12-01

    This study investigates the extent to which teachers' beliefs and classroom practices concerning inquiry-based instruction change following participation in a large mid-Atlantic university's year-long Research Experiences for Teachers (RET) - Professional Learning Community (PLC) professional development program. Mixed methods were used to explore this study's research questions. Supported with NASA funding, twelve secondary science teachers participated in the study. Study findings suggest that RET programs that incorporate a PLC component can help to shift teachers' beliefs and classroom practices concerning inquiry-based instruction, and help them to increase the level of inquiry in their science lessons. An implication of this research is that teacher professional development models need to be developed to help teachers effectively plan more time for students to conduct inquiry-based activities, to communicate findings based on evidence, and to develop questions to investigate themselves. Moreover, the findings of this study can help to inform science teacher education and professional development programs in creating more fruitful experiences for these professionals, and help them to align their beliefs and practice more toward the constructivist visions of current reform efforts.

  9. Application of PLC and Bus in Six League Outfit Kettle Sterilization%PLC及总线在六联装杀菌釜中的应用

    Institute of Scientific and Technical Information of China (English)

    赵海燕

    2012-01-01

    Based on six league outfit kettle sterilization of the characteristics of a hot water system, in order to reduce costs and increase production efficiency, enhance the stability of system as purpose, based on the Siemens PLC-S7300 programmable controller, taking advantage of the program design of the function of form, exchange products in advance to a program modification to six, save time, minimize the failure. Using the application of the bus technology to finally achieve reducing costs and concentrate control?remote control.%针对于六联装杀菌釜共用一套热水系统的特点,以降低成本和提高生产效率,增强系统稳定性为目的,基于西门子PLC-S7300可编程控制器,利用程序设计功能块化的形式,实现更换产品程序修改,以一改六,节约时间,减少故障率,并应用总线技术,最终实现降低成本和集中控制、远程监控.

  10. Systémy PLC ovládané dotekovým panelem pro řízení VN zařízení

    OpenAIRE

    Vlk, Jáchym

    2014-01-01

    Diplomová práce popisuje komunikaci PLC a dotekového displeje za účelem řízení vysokonapěťových zařízení. Je zde uveden základní přehled a stavba. Tato práce popisuje část vývoje společnosti IVEP. Jelikož se jedná o počátek dlouholetého vývoje, je předčasné, v rámci této práce, dělat cílené závěry. První kapitola je věnována PLC a dalším komponentům. Druhá kapitola je zaměřena na problematiku týkající se vysokonapěťového zařízení a dále následuje kapitola probírající programovou část. Zakonče...

  11. A Multi-rate RS Decoder for PLC System%一种用于PLC系统的多码率RS码译码器

    Institute of Scientific and Technical Information of China (English)

    石宇; 黑勇; 乔树山

    2014-01-01

    电力线通信(Power Line Communication ,PLC)凭借以电力线为通信介质,成为最具优势的通信方式。针对PLC系统中RS码多码率的问题,基于RiBM算法和uiBM 算法,设计一种适合 PLC系统的多码率RS码译码器。该译码器复杂度低,资源使用量少,易于VLSI实现。该译码器已在一款PLC芯片上得到应用。%Advantage of the power line communication with power line communication medium ,has become the most advantageous means of communication .Based on RiBM algorithms and uRiBM algorithms ,a RS decoder for PLC system with multi-rate feature is presented in this paper .With less resource and less complication ,the decoder is easy to be realized in VLSI .The decoder has been applied on a PLC chip .

  12. Transacsys PLC - acquisition

    CERN Multimedia

    2001-01-01

    Transacsys Workflow Ltd has agreed to pay 1 million Sfr to aquire the rights to the EDH suite of computer programmes developed at CERN for the administration of internal transactions. The company intends to market and sell-on EDH (1/2 page).

  13. Scoring and Classifying with Gated Auto-encoders

    OpenAIRE

    Im, Daniel Jiwoong; Taylor, Graham W.

    2014-01-01

    Auto-encoders are perhaps the best-known non-probabilistic methods for representation learning. They are conceptually simple and easy to train. Recent theoretical work has shed light on their ability to capture manifold structure, and drawn connections to density modelling. This has motivated researchers to seek ways of auto-encoder scoring, which has furthered their use in classification. Gated auto-encoders (GAEs) are an interesting and flexible extension of auto-encoders which can learn tr...

  14. Scene adaptive video encoding for MPEG and H.263+ video

    OpenAIRE

    Fernando, WAC; Canagarajah, CN

    2001-01-01

    This paper presents a new scene adaptive video encoding scheme for MPEG and H263+ video encoders. The proposed scheme determines the picture types adaptively based on statistical features of each video frame. Results show that the proposed scheme demonstrates a significant improvement in performance compared to existing schemes This paper presents a new scene adaptive video encoding scheme for MPEG and H263+ video encoders. The proposed scheme determines the picture types adaptively based ...

  15. Multiaccess Channel with Partially Cooperating Encoders and Security Constraints

    OpenAIRE

    Awan, Zohaib Hassan; Zaidi, Abdellatif; Vandendorpe, Luc

    2012-01-01

    We study a special case of Willems's two-user multi-access channel with partially cooperating encoders from a security perspective. This model differs from Willems's setup in that only one encoder, Encoder 1, is allowed to conference; Encoder 2 does not transmit any message, and there is an additional passive eavesdropper from whom the communication should be kept secret. For the discrete memoryless (DM) case, we establish inner and outer bounds on the capacity-equivocation region. The inner ...

  16. Encoding the core electrons with graph concepts.

    Science.gov (United States)

    Pogliani, Lionello

    2004-01-01

    The core electron problem of atoms in chemical graph studies has always been considered as a minor problem. Usually, chemical graphs had to encode just a small set of second row atoms, i.e., C, N, O, and F, thus, graph and, in some cases, pseudograph concepts were enough to "graph" encode the molecules at hand. Molecular connectivity theory, together with its side-branch the electrotopological state, introduced two "ad hoc" algorithms for the core electrons of higher-row atoms based, mainly, on quantum concepts alike. Recently, complete graphs, and, especially, odd complete graphs have been introduced to encode the core electrons of higher-row atoms. By the aid of these types of graphs a double-valued algorithm has been proposed for the valence delta, deltav, of any type of atoms of the periodic table with a principal quantum number n > or =2. The new algorithm is centered on an invariant suggested by the hand-shaking theorem, and the values it gives rise to parallel in some way the values derived by the aid of the two old "quantum" algorithms. A thorough comparative analysis of the newly proposed algorithms has been undertaken for atoms of the group 1A-7A of the periodic table. This comparative study includes the electronegativity, the size of the atoms, the first ionization energy, and the electron affinity. The given algorithm has also been tested with sequential complete graphs, while the even complete graphs give rise to conceptual difficulties. QSAR/QSPR studies do not show a clear-cut preference for any of the two values the algorithm gives rise to, even if recent results seem to prefer one of the two values. PMID:14741009

  17. Toward Chemical Implementation of Encoded Combinatorial Libraries

    DEFF Research Database (Denmark)

    Nielsen, John; Janda, Kim D.

    1994-01-01

    The recent application of "combinatorial libraries" to supplement existing drug screening processes might simplify and accelerate the search for new lead compounds or drugs. Recently, a scheme for encoded combinatorial chemistry was put forward to surmount a number of the limitations possessed by...... existing methodologies. Here we detail the synthesis of several matrices and the necessary chemistry to implement the conceptual scheme. In addition, we disclose how this novel technology permits a controlled ′dendritic" display of the chemical libraries. © 1994 Academic Press. All rights reserved....

  18. Error correction for encoded quantum annealing

    Science.gov (United States)

    Pastawski, Fernando; Preskill, John

    2016-05-01

    Recently, W. Lechner, P. Hauke, and P. Zoller [Sci. Adv. 1, e1500838 (2015), 10.1126/sciadv.1500838] have proposed a quantum annealing architecture, in which a classical spin glass with all-to-all pairwise connectivity is simulated by a spin glass with geometrically local interactions. We interpret this architecture as a classical error-correcting code, which is highly robust against weakly correlated bit-flip noise, and we analyze the code's performance using a belief-propagation decoding algorithm. Our observations may also apply to more general encoding schemes and noise models.

  19. Space Qualified High Speed Reed Solomon Encoder

    Science.gov (United States)

    Gambles, Jody W.; Winkert, Tom

    1993-01-01

    This paper reports a Class S CCSDS recommendation Reed Solomon encoder circuit baselined for several NASA programs. The chip is fabricated using United Technologies Microelectronics Center's UTE-R radiation-hardened gate array family, contains 64,000 p-n transistor pairs, and operates at a sustained output data rate of 200 MBits/s. The chip features a pin selectable message interleave depth of from 1 to 8 and supports output block lengths of 33 to 255 bytes. The UTE-R process is reported to produce parts that are radiation hardened to 16 Rads (Si) total dose and 1.0(exp -10) errors/bit-day.

  20. 2D Barcode for DNA Encoding

    Directory of Open Access Journals (Sweden)

    Elena Purcaru

    2011-09-01

    Full Text Available The paper presents a solution for endcoding/decoding DNA information in 2D barcodes. First part focuses on the existing techniques and symbologies in 2D barcodes field. The 2D barcode PDF417 is presented as starting point. The adaptations and optimizations on PDF417 and on DataMatrix lead to the solution – DNA2DBC – DeoxyriboNucleic Acid Two Dimensional Barcode. The second part shows the DNA2DBC encoding/decoding process step by step. In conclusions are enumerated the most important features of 2D barcode implementation for DNA.

  1. 2D Barcode for DNA Encoding

    CERN Document Server

    Purcaru, Elena

    2012-01-01

    The paper presents a solution for endcoding/decoding DNA information in 2D barcodes. First part focuses on the existing techniques and symbologies in 2D barcodes field. The 2D barcode PDF417 is presented as starting point. The adaptations and optimizations on PDF417 and on DataMatrix lead to the solution - DNA2DBC - DeoxyriboNucleic Acid Two Dimensional Barcode. The second part shows the DNA2DBC encoding/decoding process step by step. In conclusions are enumerated the most important features of 2D barcode implementation for DNA.

  2. 2D Barcode for DNA Encoding

    OpenAIRE

    Elena Purcaru; Cristian Toma

    2012-01-01

    The paper presents a solution for endcoding/decoding DNA information in 2D barcodes. First part focuses on the existing techniques and symbologies in 2D barcodes field. The 2D barcode PDF417 is presented as starting point. The adaptations and optimizations on PDF417 and on DataMatrix lead to the solution – DNA2DBC – DeoxyriboNucleic Acid Two Dimensional Barcode. The second part shows the DNA2DBC encoding/decoding process step by step. In conclusions are enumerated the most important features ...

  3. Wavelet encoding and variable resolution progressive transmission

    Science.gov (United States)

    Blanford, Ronald P.

    1993-01-01

    Progressive transmission is a method of transmitting and displaying imagery in stages of successively improving quality. The subsampled lowpass image representations generated by a wavelet transformation suit this purpose well, but for best results the order of presentation is critical. Candidate data for transmission are best selected using dynamic prioritization criteria generated from image contents and viewer guidance. We show that wavelets are not only suitable but superior when used to encode data for progressive transmission at non-uniform resolutions. This application does not preclude additional compression using quantization of highpass coefficients, which to the contrary results in superior image approximations at low data rates.

  4. Spatially encoded multiple-quantum excitation

    Science.gov (United States)

    Ridge, Clark D.; Borvayeh, Leila; Walls, Jamie D.

    2013-05-01

    In this work, we present a simple method to spatially encode the transition frequencies of nuclear spin transitions and to read out these frequencies within a single scan. The experiment works by combining pulsed field gradients with an excitation sequence that selectively excites spin transitions within certain sample regions. After the initial excitation, imaging the resulting widehat{z}-magnetization is used to determine the locations where the excitations occurred, from which the corresponding transition frequencies are determined. Simple experimental demonstrations of this technique on one- and two-spin systems are presented.

  5. Aging, encoding quality and false memories

    OpenAIRE

    Dehon, Hedwige; Brédart, Serge

    2005-01-01

    The current experiment explored the effect of enhancing the quality of encoding on the creation of false memories in older participants. Three groups of participants (1 group of younger adults and two groups of older adults) were presented with a modified version (Brédart, 2000) of the DRM paradigm (Roediger & McDermott, 1995) which permitted to elicit false recall. We used a short interstimuli interval (ISI of 1.5 sec) in the younger and the older control group. A slower rate of presentation...

  6. DVB-S2 FEC Encoder: Implementation of DVB-S2 FEC encoder in FPGA

    OpenAIRE

    Myhr, Reidar

    2007-01-01

    This Master Thesis describe how DVB-S2 Forward Error Correction (FEC) encoding can be implemented in hardware like a FPGA. It include the design, simulation, verification and synthesis of a complete encoder for the DVB-S2 FEC system. First the Thesis gives a introduction to the history behind DVB-S2 and the FEC system. It describe shortly the theory behind the error correcting codes used in the FEC, BCH and LDPC codes. To get an effective implementation in hardware it was necessary to studies...

  7. Reading Neural Encodings using Phase Space Methods

    CERN Document Server

    Abarbanel, Henry D I; Abarbanel, Henry D I; Tumer, Evren C.

    2003-01-01

    Environmental signals sensed by nervous systems are often represented in spike trains carried from sensory neurons to higher neural functions where decisions and functional actions occur. Information about the environmental stimulus is contained (encoded) in the train of spikes. We show how to "read" the encoding using state space methods of nonlinear dynamics. We create a mapping from spike signals which are output from the neural processing system back to an estimate of the analog input signal. This mapping is realized locally in a reconstructed state space embodying both the dynamics of the source of the sensory signal and the dynamics of the neural circuit doing the processing. We explore this idea using a Hodgkin-Huxley conductance based neuron model and input from a low dimensional dynamical system, the Lorenz system. We show that one may accurately learn the dynamical input/output connection and estimate with high precision the details of the input signals from spike timing output alone. This form of "...

  8. Linear encoder based low frequency inertial sensor

    Directory of Open Access Journals (Sweden)

    Collette Christophe

    2015-01-01

    Full Text Available For many applications, there is an increasing demand for low cost, high-resolution inertial sensors, which are capable of operating in harsh environments. Recently, a prototype of small optical inertial sensor has been built, using a Michelson interferometer. A resolution of 3 pm/√Hz has been obtained above 4 Hz using only low cost components. Compared to most state-of-the-art devices, this prototype did not contain any coil, which offers several important advantages, including a low thermal noise in the suspension and a full compatibility with magnetic environments (like particle collider. On the other hand, the Michelson is known to be tricky to tune, especially when one attempts to miniaturize the sensor. In this paper, we will propose a novel concept of inertial sensor, based on a linear encoder. Compared to the Michelson, the encoder is much more easy to mount, and the calibration more stable. The price to pay is a reduced resolution. In order to overcome this limitation, we amplify mechanically the relative motion between the support and the inertial mass. First results obtained with the new sensor will be discussed, and compared with the Michelson inertial sensor.

  9. V123 Beam Synchronous Encoder Module

    International Nuclear Information System (INIS)

    The V123 Synchronous Encoder Module transmits events to distributed trigger modules and embedded decoders around the RHIC rings where they are used to provide beam instrumentation triggers [1,2,3]. The RHIC beam synchronous event link hardware is mainly comprised of three VMEbus board designs, the central input modules (V201), and encoder modules (V123), and the distributed trigger modules (V124). Two beam synchronous links, one for each ring, are distributed via fiberoptic and fanned out via twisted wire pair cables. The V123 synchronizes with the RF system clock derived from the beam bucket frequency and a revolution fiducial pulse. The RF system clock is used to create the beam synchronous event link carrier and events are synchronized with the rotation fiducial. A low jitter RF clock is later recovered from this carrier by phase lock loops in the trigger modules. Prioritized hardware and software triggers fill up to 15 beam event code transmission slots per revolution while tracking the ramping RF acceleration frequency and storage frequency. The revolution fiducial event is always the first event transmitted which is used to synchronize the firing of the abort kicker and to locate the first bucket for decoders distributed about the ring

  10. Spectrally encoded confocal scanning laser ophthalmoscope

    Science.gov (United States)

    Tao, Yuankai K.; Izatt, Joseph A.

    2010-02-01

    Fundus imaging has become an essential clinical diagnostic tool in ophthalmology. Current generation scanning laser ophthalmoscopes (SLO) offer advantages over conventional fundus photography and indirect ophthalmoscopy in terms of light efficiency and contrast. As a result of the ability of SLO to provide rapid, continuous imaging of retinal structures and its versatility in accommodating a variety of illumination wavelengths, allowing for imaging of both endogenous and exogenous fluorescent contrast agents, SLO has become a powerful tool for the characterization of retinal pathologies. However, common implementations of SLO, such as the confocal scanning laser ophthalmoscope (CSLO) and line-scanning laser ophthalmoscope (LSLO), require imaging or multidimensional scanning elements which are typically implemented in bulk optics placed close to the subject eye. Here, we apply a spectral encoding technique in one dimension combined with single-axis lateral scanning to create a spectrally encoded confocal scanning laser ophthalmoscope (SECSLO) which is fully confocal. This novel implementation of the SLO allows for high contrast, high resolution in vivo human retinal imaging with image transmission through a single-mode optical fiber. Furthermore, the scanning optics are similar and the detection engine is identical to that of current-generation spectral domain optical coherence tomography (SDOCT) systems, potentially allowing for a simplistic implementation of a joint SECSLO-SDOCT imaging system.

  11. Multiple-encoding retrieval for optical security

    Science.gov (United States)

    Barrera, John Fredy; Henao, Rodrigo; Tebaldi, Myrian; Torroba, Roberto; Bolognini, Néstor

    2007-08-01

    We propose a method for image encryption by multiple-step random phase encoding with an undercover multiplexing operation. The true image is stored in a multiple record we call encodegram; and then we can reconstruct it by the use of the appropriate random phase masks and a retrieval protocol. To increase the security of the true hidden image and confuse unauthorized receivers, we add to the encodegram an encoded fake image with different content. This fake image has only a small effect on the retrieval of the true hidden image, owing to the specific property of this protocol. In the decryption step, we can reveal the true image by applying the inverse protocol to two cyphertexts, one the encodegram containing the true image along with the fake image; and the other helping to get the random phase key to achieve the true image. Computer simulations verify the validity of this method for image encryption. Digital implementation of the method makes it particularly suitable for the remote transmission of information.

  12. An integrated environment of software development and V and V for PLC based safety-critical systems

    International Nuclear Information System (INIS)

    To develop and implement a safety-critical system, the requirements of the system must be analyzed thoroughly during the phases of a software development's life cycle because a single error in the requirements can generate serious software faults. We therefore propose an Integrated Environment (IE) approach for requirements which is an integrated approach that enables easy inspection by combining requirement traceability and effective use of a formal method. For the V and V tasks of requirements phase, our approach uses software inspection, requirement traceability, and formal specification with structural decomposition. Software inspection and the analysis of requirements traceability are the most effective methods of software V and V. Although formal methods are also considered an effective V and V activity, they are difficult to use properly in nuclear fields, as well as in other fields, because of their mathematical nature. We also propose another Integrated Environment (IE) for the design and implementation of safety-critical systems. In this study, a nuclear FED-style design specification and analysis (NuFDS) approach was proposed for PLC based safety-critical systems. The NuFDS approach is suggested in a straightforward manner for the effective and formal specification and analysis of software designs. Accordingly, the proposed NuFDS approach comprises one technique for specifying the software design and another for analyzing the software design. In addition, with the NuFDS approach, we can analyze the safety of software on the basis of fault tree synthesis. To analyze the design phase more effectively, we propose a technique of fault tree synthesis, along with a universal fault tree template for the architecture modules of nuclear software. Various tools have been needed to make software V and V more convenient. We therefore developed four kinds of computer-aided software engineering tools that could be used in accordance with the software's life cycle to

  13. On the detection of differentially encoded polyphase signals.

    Science.gov (United States)

    Lindsey, W. C.; Simon, M. K.

    1972-01-01

    Consideration of the transmission and detection of differentially encoded multiple phase-shift-keyed (MPSK) signals, paying particular attention to the ambiguity resolution problem resulting from suppression of the transmitted carrier. A study is made of the coherent detection of differentially encoded MPSK signals, and the performance of a differentially encoded MPSK system is compared with that of a system which transmits absolutely encoded polyphase signals and performs perfect ambiguity resolution. Both the perfect and noisy reference signal cases are treated. Also, the performance of coherent detection of differentially encoded MPSK signals is compared with that of differentially coherent reception of the same signal set.

  14. Device Development for Electrotechnical Practice Teaching Based on PLC%基于PLC的电工实习教学装置的研发

    Institute of Scientific and Technical Information of China (English)

    赵春锋; 陈国民; 范小兰; 余粟

    2013-01-01

    In order to improve the teaching condition of the practical training base in Shanghai University of Engineering Science and to explore the role of electrical engineering practice in the cultivation of the innovative ability of application-oriented undergraduates, a high performance electrical practice teaching device in line with modern electrical technology theory and the electric control principle based PLC was researched and developed. The device has PLC, frequency converter, relay, sensor, computer and configuration software and other common low-voltage electrical appliances. This paper introduces the design ideas, design scheme and the practice content. By means of the practice training using this device, the students can understand the composition of the electricity control system, have electrical operation skills, the low voltage electrical system installation and debugging abilities and the ability to solve practical problems. The device links classroom teaching closely with engineering practice, facilitating the cultivattion of students' engineering practice and innovation ability.%为了改善我校教学实习基地办学条件,积极探索电工实习在培养具有创新能力的本科应用型人才中的工程教育作用,开发研究出符合现代电工技术理论和电气控制原理的基于PLC的高性能电工实习教学装置.该装置具有PLC,变频器,继电接触器,传感器,计算机及组态软件等常用低压电气控制系统配置.作者介绍其设计思想、总体设计方案及所能开设的实习内容.通过该装置实习训练,让学生掌握常用的电气控制系统组成,初步具有电工操作技能、低压电气系统的安装调试能力和解决实际问题的能力.该装置将教学紧密与工程实践结合,为培养学生工程实践能力、科技创新能力提供了良好的条件.

  15. Non-Fourier-encoded parallel MRI using multiple receiver coils.

    Science.gov (United States)

    Mitsouras, Dimitris; Hoge, W Scott; Rybicki, Frank J; Kyriakos, Walid E; Edelman, Alan; Zientara, Gary P

    2004-08-01

    This paper describes a general theoretical framework that combines non-Fourier (NF) spatially-encoded MRI with multichannel acquisition parallel MRI. The two spatial-encoding mechanisms are physically and analytically separable, which allows NF encoding to be expressed as complementary to the inherent encoding imposed by RF receiver coil sensitivities. Consequently, the number of NF spatial-encoding steps necessary to fully encode an FOV is reduced. Furthermore, by casting the FOV reduction of parallel imaging techniques as a dimensionality reduction of the k-space that is NF-encoded, one can obtain a speed-up of each digital NF spatial excitation in addition to accelerated imaging. Images acquired at speed-up factors of 2x to 8x with a four-element RF receiver coil array demonstrate the utility of this framework and the efficiency afforded by it. PMID:15282814

  16. Encoding and decoding time in neural development.

    Science.gov (United States)

    Toma, Kenichi; Wang, Tien-Cheng; Hanashima, Carina

    2016-01-01

    The development of a multicellular organism involves time-dependent changes in molecular and cellular states; therefore 'time' is an indispensable mathematical parameter of ontogenesis. Regardless of their inextricable relationship, there is a limited number of events for which the output of developmental phenomena primarily uses temporal cues that are generated through multilevel interactions between molecules, cells, and tissues. In this review, we focus on neural stem cells, which serve as a faithful decoder of temporal cues to transmit biological information and generate specific output in the developing nervous system. We further explore the identity of the temporal information that is encoded in neural development, and how this information is decoded into various cellular fate decisions. PMID:26748623

  17. Photoelectrical encoder employing an optical grating

    Energy Technology Data Exchange (ETDEWEB)

    Kabaya, Y.

    1985-02-12

    A photoelectrical encoder is disclosed wherein a physical quantity is detected from brightness obtained by moving a first and a second scale. Each scale is provided thereon with an optical grating relative to each other. The grating in one of the scales is constructed such that a first signal lead-out material layer made of a light shielding conductive material, a PN semiconductor layer for converting light into electricity, and a second signal lead-out material layer made of a light transmitting conductive material are laminated on a light transmitting base member to form a narrow belt-shaped light receiving portion and a plurality of narrow belt-shaped light receiving portions arranged at regular pitches. Against the light from the light transmitting base member, the light receiving portions function as light shielding slits, and intervals between the light receiving portions are formed into light transmitting slits.

  18. Radiofrequency encoded angular-resolved light scattering

    DEFF Research Database (Denmark)

    Buckley, Brandon W.; Akbari, Najva; Diebold, Eric D.;

    2015-01-01

    The sensitive, specific, and label-free classification of microscopic cells and organisms is one of the outstanding problems in biology. Today, instruments such as the flow cytometer use a combination of light scatter measurements at two distinct angles to infer the size and internal complexity...... of cells at rates of more than 10,000 per second. However, by examining the entire angular light scattering spectrum it is possible to classify cells with higher resolution and specificity. Current approaches to performing these angular spectrum measurements all have significant throughput limitations...... Encoded Angular-resolved Light Scattering (REALS), this technique multiplexes angular light scattering in the radiofrequency domain, such that a single photodetector captures the entire scattering spectrum from a particle over approximately 100 discrete incident angles on a single shot basis. As a proof...

  19. Implementation of PFC (Predictive Functional Control) in a PLC (Programmable Logic Controller) for a HVAC (Heating, Ventilation and Air Conditioning) system

    Science.gov (United States)

    Kreutz, M.; Richalet, J.; Mocha, K.; Haber, R.

    2014-12-01

    HVAC systems of industrial buildings consume a lot of energy. Therefore it is important to know the performance of these systems and strategies to optimize the hardware and the control. Tackling the temperature control of the HVAC system promises quick savings by tuning the control within specified tolerance limits, which mostly can be done by low investment. This paper mainly deals with the implementation strategy of a new controller in a PLC using the predictive functional control for temperature control. The different stages of the implementation from the simulation over the SCL code till to the real-time operation are presented. A bumpless switch between the PI(D) and the PFC control was realized, as well.

  20. Optimization Design of Traffic Light Control Program Based PLC%基于PLC的交通灯控制程序优化设计

    Institute of Scientific and Technical Information of China (English)

    刘爱云

    2012-01-01

    Based on Siemens S7-200 PLC instruction system for crossroads traffic lights control program design, my paper gives three different design methods which use of bit logic instruction, sequential control instruction and compare instruction. By comparing, the method which using compare instruction is more simple and easy to understand.%文章利用西门子S7 -200PLC指令系统进行十字路口交通灯控制的程序设计,给出利用位逻辑指令、顺控指令、比较指令三种不同的设计思路,通过对比,利用比较指令实现的程序,更加简单和容易理解.