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  1. Alpha-Fetoprotein Detection of Hepatocellular Carcinoma Leads to a Standardized Analysis of Dynamic AFP to Improve Screening Based Detection.

    Science.gov (United States)

    Bird, Thomas G; Dimitropoulou, Polyxeni; Turner, Rebecca M; Jenks, Sara J; Cusack, Pearce; Hey, Shiying; Blunsum, Andrew; Kelly, Sarah; Sturgeon, Catharine; Hayes, Peter C; Bird, Sheila M

    2016-01-01

    Detection of hepatocellular carcinoma (HCC) through screening can improve outcomes. However, HCC surveillance remains costly, cumbersome and suboptimal. We tested whether and how serum Alpha-Fetoprotein (AFP) should be used in HCC surveillance. Record linkage, dedicated pathways for management and AFP data-storage identified i) consecutive highly characterised cases of HCC diagnosed in 2009-14 and ii) a cohort of ongoing HCC-free patients undergoing regular HCC surveillance from 2009. These two well-defined Scottish patient cohorts enabled us to test the utility of AFP surveillance. Of 304 cases of HCC diagnosed over 6 years, 42% (129) were identified by a dedicated HCC surveillance programme. Of these 129, 47% (61) had a detectable lesion first identified by screening ultrasound (US) but 38% (49) were prompted by elevated AFP. Despite pre-HCC diagnosis AFP >20kU/L being associated with poor outcome, 'AFP-detected' tumours were offered potentially curative management as frequently as 'US-detected' HCCs; and had comparable survival. Linearity of serial log10-transformed AFPs in HCC cases and in the screening 'HCC-free' cohort (n = 1509) provided indicators of high-risk AFP behaviour in HCC cases. An algorithm was devised in static mode, then tested dynamically. A case/control series in hepatitis C related disease demonstrated highly significant detection (pAFP in HCC surveillance. We show proof-of-principle that an automated and further refine-able algorithmic interpretation of AFP can identify patients at higher risk of HCC. This approach could provide a cost-effective, user-friendly and much needed addition to US surveillance. PMID:27308823

  2. Alpha fetoprotein

    Science.gov (United States)

    Fetal alpha globulin; AFP ... Greater than normal levels of AFP may be due to: Cancer in testes , ovaries, biliary (liver secretion) tract, stomach, or pancreas Cirrhosis of the liver Liver cancer ...

  3. The diagnostic value of combined detection of alpha-fetoprotein, AFP, lentil lectin-reactive alpha-fetoprotein(AFP-L3) and alpha L fucosidase (AFU) level for the patients with primary liver cancer in early stage%联合检测血清AFP、AFP异质体和AFU对早期肝癌诊断价值的研究

    Institute of Scientific and Technical Information of China (English)

    王杰; 赵业民; 陈增银; 史光军

    2013-01-01

    Objective To explore the diagnostic value of combined detection of serum alpha-fetoprotein ( AFP), lentil lectin-reactive alpha-fetoprotein( AFP-L3 ) and alpha L fucosidase ( AFU ) level for the patients with primary liver cancer in early stage. Methods The serum level of AFP, AFP-L3 and AFU were detected in 56 patients with primary liver cancer (PLC), 158 patients with benign liver disease, 40 patients with other malignant tumor without PLC and 40 healthy persons.Results The positive rate of AFP, AFP-L3 and AFU in the group of PLC were signiifcantly higher than that of in the group of benign liver diseases and in the group of other malignant tumor without PLC ( P<0.01 ). Combined detection have a complementary function for the diagnosis of patients PLC in early stage and the sensitivity rate was 94.60%. The sensitivity in the group of combined detection is higher than that of in the group of single detection ( P<0.05 ). Conclusion The sensitivity rate of single detection for AFP, AFP-L3 and AFU for the diagnosis of PLC in the early stage is similar and combined detection could signiifcantly improve the sensitivity rate for the diagnosis of PLC in the early stage and decrease the misdiagnosis rate. The combined detection of AFP, AFP-L3 and AFU can be used as a newly routine detection method for the high population of the patients with PLC in the early stage especially.%目的:探讨血清甲胎蛋白(alpha-fetoproteinAFP)、扁豆凝集素亲和型甲胎蛋白异质体(lentil lectin-reactive alpha-fetoprotein, AFP-L3)、α-L-岩藻糖苷酶(alpha L fucosidase, AFU)水平联合检测对原发性肝癌(primary liver cancer, PLC)的诊断价值。方法对56例PLC、158例良性肝病患者、40例非PLC恶性肿瘤患者以及40例健康查体者进行AFP、AFP-L3和AFU进行联合检测。结果 PLC组的AFP、AFP-L3、AFU的阳性率明显高于良性肝病和非PLC恶性肿瘤组( P<0.01),PLC患者血清AFP、AFP-L3、AFU三者之间无相关性,联

  4. Hepatoid carcinoma of the pancreas producing protein induced by vitamin K absence or antagonist II (PIVKA-II) and alpha-fetoprotein (AFP).

    Science.gov (United States)

    Matsueda, Kazuhiro; Yamamoto, Hiroshi; Yoshida, Yasuo; Notohara, Kenji

    2006-10-01

    We describe a rare case of hepatoid carcinoma of the pancreas with production of protein induced by vitamin K absence or antagonist II (PIVKA-II) and alpha-fetoprotein (AFP). The patient was a 49-year-old woman admitted because of high serum levels of PIVKA-II (1.63 AU/ml) and AFP (623 ng/ml) and abnormal ultrasonographic findings of the pancreas, found incidentally at medical checkup. Both ultrasonography and computed tomography showed swelling of the pancreas with small areas of low density, but no hepatic lesions. The serum levels of carcinoembryonic antigen and carbohydrate antigen 19-9 were not increased. A PIVKA-II and AFP-producing pancreatic cancer was strongly suspected, and total pancreatectomy was performed. Pathological examination showed that the tumor cells were arranged in trabecular and solid patterns with bile production, and were immunohistochemically positive for PIVKA-II and AFP, resembling hepatocellular carcinoma cells. The tumor was diagnosed as hepatoid carcinoma of the pancreas, and the patient has survived 48 months after initial diagnosis. It is important that hepatoid carcinoma be considered as a possible malignant tumor of the pancreas, and simultaneous measurement of the serum levels of AFP and PIVKA-II will enable earlier diagnosis. This is the first report describing hepatoid carcinoma of the pancreas producing PIVKA-II.

  5. Elevated Serum Levels of Alpha-Fetoprotein in Patients with Infantile Hemangioma Are Not Derived from within the Tumor

    OpenAIRE

    Itinteang, Tinte; Chibnall, Alice M.; Marsh, Reginald; Jonathan C. Dunne; de Jong, Sophie; Davis, Paul F.; Leadbitter, Philip; Tan, Swee T.

    2016-01-01

    Aims The embryonic-like stem cell origin of infantile hemangioma (IH) and the observed elevated serum levels of alpha-fetoprotein (AFP) in patients with hepatic IH led us to investigate if this tumor was the source of AFP. Materials and methods We measured serial serum levels of AFP in patients with problematic proliferating IH treated with surgical excision or propranolol treatment. We also investigated the expression of AFP in extrahepatic IH samples using immunohistochemical sta...

  6. AFP、APF-L3、GP73、AFP-mRNA联合检测在肝细胞癌诊断中的应用价值%The application value of combined detection for serum alpha-fetoprotein, alpha-fetoprotein-L3, Golgi protein 73 and AFP-mRNA in the diagnosis of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    李永勤; 陈浩; 耿坤静; 杜博勋; 裘银久

    2012-01-01

    Objective To investigate the application value of combined detection for alpha fetoprotein (AFP), alpha-fetoprotein-L3 ( AFP-L3 ) , Golgi protein 73 ( GP73 ) and AFP-mRNA in the diagnosis of hepatocellular carcinoma ( HCC). Methods Enzyme-linked immunosorbent assay ( ELISA) was used to detect the serum levels of GP73 in 73 cases of HCC,41 cases of liver cirrhosis,36 cases of chronic hepatitis B and 25 healthy subjects. AFP was detected by chemiluminescent immunoassay, and AFP-L3 was determined by adsorption centrifuging. AFP-mRNA was tested by fluorescent quantitative RT-PCR. Results There were significant differences in the levels of AFP, AFP-L3, GP73, AFP-mRNA between HCC group and cirrhosis group, normal control group ( P <0.01). The areas evaluated according to ROC curves were 0.691 ( AFP) ,0.859 ( APF-L3), 0.816 (GP73),0.762 (AFP-mRNA) respectively in HCC group,and the sensitivities were 54. 79% ,76. 71% , 80.82% ,53. 42%, respectively, and specificities were 79. 41% ,95. 10% ,82. 35% ,99. 02% , respectively. The areas under ROC curves for the four tumor-related markers were 0.958 by combined detection. The sensitivity of combined detection was significantly increased, which reached 95. 8% , and its specificity was 95. 0% in the diagnosis of HCC. Conclusion The combination detection for AFP, AFP-L3 ,GP73 and AFP-mRNA can obviously enhance the sensitivity and specificity in the diagnosis of HCC,which is superior to individual detection of AFP, AFP-L3.GP73 or AFP-mRNA.%目的 探讨甲胎蛋白(AFP)、甲胎蛋白异质体( AFP-L3)、高尔基体蛋白(GP73)及AFP-mR-NA联合检测在HCC诊断中的价值.方法 收集175例血清、其中肝细胞癌患者73例(HCC组)、肝硬化患者41例(肝硬化组)、慢乙肝患者36例(慢性肝炎组)、正常人25例(正常人组),采用酶联免疫法检测GP73,用化学发光法检测AFP,用亲和吸附离心法检测AFP-L3,采用荧光定量逆转录聚合酶链反应(RTPCR)法检测患者AFP

  7. Application of Latex particles in detecting alpha- fetoprotein (AFP)%乳胶粒子法检测甲胎蛋白的应用

    Institute of Scientific and Technical Information of China (English)

    陈浩全; 曾嫚妮

    2012-01-01

    Objective:To evaluate the application value of Latex method in detection of alpha - fetoprotein ( AFP). Methods:Repetitive determination tests within and between batches were done on serum specimens, then the determination results were compared and analyzed by Latex method and electrochemiluminescence assay. Results; The results had no significant difference between Latex method and electrochemiluminescence assay, the recoveries were 95.5% for Latex method and 96.7% for electrochemiluminescence assay. Conclusion; Though electrochemiluminescence assay had high sensitivity and precision, it was not suitable for the wide use due to its high cost in instrument and reagent. Latex method had good correlation with electrochemiluminescence assay, which can be applied widely for its rapidness, stable results and low cost.%目的:评价Latex法检测甲胎蛋白(AFP)的临床应用价值.方法:对血清标本做批内批间重复性测定试验及Latex法与电化学发光法进行对比测定.结果:Latex法与电化学发光法检测AFP结果无显著差异,Latex法与电化学发光法平均回收率分别为95.5%和96.7%.结论:电化学发光法灵敏度、精密度高,但其仪器、试剂价格高,不适于全面推广;Latex法与电化学发光法检测AFP结果无显著差异,相关性好,快速、结果稳定,试剂便宜在全自动生化仪上检测,可广泛应用.

  8. Clinical evaluation of serum alpha-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) levels in patients with hepatocellular carcinoma following interventional radiology.

    Science.gov (United States)

    Minakuchi, K; Murata, K; Kaminoh, T; Takada, K; Takashima, S; Nakamura, K; Onoyama, Y

    1993-01-01

    Fourteen patients with unrespectable HCC were treated with various interventional radiology (IVR) procedures. The initial therapeutic response was determined using computed tomography (CT) findings, and determinations of serum alpha-fetoprotein (AFP) and protein induced by Vitamin K absence or antagonist-II (PIVKA-II) levels. When CT studies of the initial response to IVR were compared with changes in the serum AFP and PIVKA-II levels, the AFP level was found to correlate more closely than the PIVKA-II levels. The PIVKA-II level correlated more closely than the AFP level in cases with poor response to IVR. Both of these tumor markers should be measured in combination with the diagnostic imagings for follow-up studies of IVR.

  9. Clinical significance of human alpha-fetoprotein

    Energy Technology Data Exchange (ETDEWEB)

    Yachnin, S.

    1978-01-01

    Deviations from the normal of alpha-fetoprotein (AFP) concentrations in fetal serum, amniotic fluid, maternal serum and adult human serum can be explained by understanding the normal physiology and the pathophysiology of AFP synthesis and metabolism. AFP is the prototype of oncofetal markers. Emphasis is given to the usefulness of elevated serum AFP levels in the diagnosis and management of primary hepatomas and tumors of germ cell origin. The ability to detect neural tube defects early in gestation by monitoring maternal serum and amniotic fluid AFP concentrations is discussed.

  10. [Alpha Fetoprotein-producing Lung Adenocarcinoma].

    Science.gov (United States)

    Komori, Kazuyuki; Tabata, Toshiharu; Sato, Kimiaki; Katsumata, Hiroshi; Minowa, Muneo; Kondo, Takashi

    2015-11-01

    We report a case of alpha fetoprotein (AFP) -producing lung adenocarcinoma. A 53-year-old man was referred to our hospital due to right pneumothorax. Computed tomography showed right moderate pneumothorax, a solid tumor in the upper lobe (S3) and mediastinal lymph node swelling. The serum AFP level was as high as 223.0 ng/ml. Frozen examination revealed a low-differentiated adenocarcinoma. Based on the pathological and immunohistochemical findings, the tumor was diagnosed as AFP-producing lung adenocarcinoma.

  11. Hereditary persistence of alpha-fetoprotein (HPAFP) : review of the literature

    NARCIS (Netherlands)

    Houwert, A. C.; Giltay, J. C.; Lentjes, E. G. W. M.; Lock, M. T. W. T.

    2010-01-01

    Alpha-fetoprotein (AFP) serum levels are raised in several clinical conditions, ranging from non-pathological conditions to malignancies. Hereditary persistence of alpha-fetoprotein (HPAFP) is a rare benign disorder with elevated AFP levels. HPAFP is described as a benign autosomal dominantly inheri

  12. Diagnostic Value of Des-gamma Carboxyprothrombin(DCP), Lectin-bound AFP(AFP-L3) and Alpha-fetoprotein (AFP) Expression in Primary Hepatocellular Carcinoma%DCP、AFP-L3和AFP在原发性肝细胞癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    付水; 齐娟飞; 朱海燕; 祁志荣; 陈红艳

    2013-01-01

    Objective To observe the alone and joint diagnostic value of des-gamma carboxyprothrombin (DCP) , lectin-bound AFP(AFP-13) and alpha-fetoprotein(AFP) in primary hepatocellular carcinoma, and provide a novel method for diagnosis for PHC and screening for high risk population. Methods The levels of serum DCP,AFP-L3 and AFP were measured in 53 patients with hepa-tocellular carcinoma, 51 cases of cirrhosis, 60 cases of chronic hepatitis, 52 normal healthy subjects established as control group, and analyse the result statistically. Results The levels of serum DCP,AFP-L3 and AFP were significantly higher in the hepatocellular carcinoma than those in other groups. The diagnostic sensitivities of DCP,AFP-I3 and AFP to hepatocellular carcinoma were 77.36% , 69.81% and 60.38% respectively, the specificity were 89.57% , 88.34% and 77.91% , individually. The joint detection could improve sensitivity up to 94.34%. Conclusion DCP was a high sensitivity and specificity marker for diagnosis of PHC. The combined assay of serum tumor markers possess more value in the diagnosis of PHC, especially in AFP negative patients.%目的:探讨血清中脱γ一羧基凝血酶原(DCP)、甲胎蛋白异质体(AFP-L3)和甲胎蛋白(AFP)对原发性肝细胞癌(PHC)单独和联合诊断的意义,为PHC诊治提供一种新方法,以及对高危人群作筛选.方法:采集 53 例PHC、51 例肝硬化、60 例慢性肝炎和 52 例健康对照者的血清分别检测 DCP、AFP-L3 和 AFP,并对其统计分析.结果:PHC患者的 DCP、AFP-L3 和 AFP 均显著高于肝硬化、慢性肝炎和健康对照者,DCP、AFP-L3 和 AFP在PHC组中的灵敏度分别为 77.36 %、69.8 1% 和60.38 %,特异性分别为89.57 %、88.34 % 和 77.91 %.三者联检时,其诊断的敏感度可提高至 94.34 %.结论:DCP 对PHC诊断具有较好的敏感度和特异性,联检DCP、AFP-L3和AFP可有效提高PHC尤其是AFP阴性PHC的诊断效率,对PHC的早期诊治具有一定指导意义.

  13. AFP-L3在肝细胞癌诊断中的临床价值%Diagnostic role of alpha-fetoprotein variants in hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    韩鹏; 褚瑞海; 张彩彩; 郭欣

    2012-01-01

    目的 探讨血清中AFP-L3含量对肝细胞癌诊断的临床意义.方法 利用微量离心柱法分离得到AFP-L3,采用化学发光法分别检测总AFP与AFP-L3含量,进而计算AFP-L3与总AFP的比值.结果 肝细胞癌患者血清中AFP及AFP-L3的水平均显著高于其他各组,而不同AFP浓度的肝细胞癌患者组AFP-L3的阳性率无明显差异.结论 AFP-L3作为肿瘤标志物对肝细胞癌的早期诊断及良、恶性肝病的鉴别有一定的临床价值.

  14. ORIGIN OF RAISED MATERNAL SERUM ALPHA-FETOPROTEIN LEVELS IN 2ND-TRIMESTER OLIGOHYDRAMNIOS

    NARCIS (Netherlands)

    LOS, FJ; BEEKHUIS, [No Value; MARRINK, J; HAGENAARS, AM; REUSS, A; SACHS, ES; JAHODA, MGJ; WLADIMIROFF, JW

    1992-01-01

    Concanavalin A (Con A) subtyping of alpha-fetoprotein (AFP) revealed higher concentrations of AFP non-reactive with Con A in sera of 12 pregnant women with second-trimester oligohydramnios and raised total serum AFP levels than in sera of 42 pregnant women with raised total serum AFP levels and a no

  15. A resected case of metachronous liver metastasis from lung cancer producing alpha-fetoprotein (AFP) and protein induced by vitamin K absence or antagonist II (PIVKA-II).

    Science.gov (United States)

    Oshiro, Yukio; Takada, Yasutsugu; Enomoto, Tsuyoshi; Fukao, Katashi; Ishikawa, Shigemi; Iijima, Tatsuo

    2004-01-01

    A resected case of huge liver metastasis of hepatoid adenocarcinoma of the lung is described. A 77-year-old man who presented a solitary huge liver tumor was admitted to our hospital. He had undergone right lower lobectomy of the lung for lung cancer one year before. The view of imaging studies was not a typical one of hepatocellular carcinoma. Serum levels of AFP and PIVKA-II were 334,500ng/mL and 3,890mAU/mL, respectively, and the proportion of AFP L3 was 97.9%. It was thought that they were strongly diagnostic for hepatocellular carcinoma. Extended right lobectomy of the liver was performed. Microscopically, it was poorly differentiated adenocarcinoma and diagnosed as liver metastasis from the formerly resected lung cancer. The tumor was composed of cells with both sheet-like growth and tubule formation. The neoplastic cells, in the sheet-like growth resembled hepatocellular carcinoma cells. By immunohistochemical staining with anti-AFP and anti-PIVKA-II antibodies, cancer cells of both the primary and metastatic lesions were positive. The patient eventually died of multiple liver and bone metastasis 6 months after the operation.

  16. Dexamethasone inhibits alpha-fetoprotein gene transcription in neonatal rat liver and isolated nuclei.

    OpenAIRE

    Huang, D. P.; Cote, G J; Massari, R J; Chiu, J F

    1985-01-01

    The effect of dexamethasone on rat alpha-fetoprotein (AFP) expression has been further examined. Quantitation of serum AFP levels from newborns treated with dexamethasone showed a dose-response relationship between the quantity of dexamethasone administered and the reduction in AFP serum level. RNA blots, utilizing cloned AFP cDNA as probe, showed a marked reduction in AFP mRNA in dexamethasone treated livers. The extent of AFP mRNA depletion was correlated with dexamethasone dosage. The effe...

  17. Evaluation of a second trimester triple marker screening test for fetal status using alpha-fetoprotein (aFP), human chorionic gonadotropin (hCG) and unconjugated estriol (uE{sub 3})

    Energy Technology Data Exchange (ETDEWEB)

    Mi, Seong Young; Kim, Jong Ho; Choi, Seung Hun [Chung Ang Gil Hospital, Inchon (Korea, Republic of)

    1997-07-01

    Our purpose was to assess the utility of maternal serum triple-marker screening test using alpha-fetoprotein (aFP), human Chorionic Gonadotropin (hCG) and unconjugated Estriol (uE{sub 3}) for fetal chromosomal abnormalities. 1,767 venous blood samples (4ml) between 15 and 20 week's gestation for maternal serum screening from January to October 1996, were tested with Kodak Amerix-M triple marker radioimmunoassay kits. Risk analysis was achieved with interpretive software such as Alpha (LMS, Kodak Clinical Diagnostics). Marker levels are transformed into multiples of median (MOM), which represent an interpretation of (weight regressed) patient marker levels relative to regressed median levels for stated gestation. By multivariate anaysis, the three MOM values are combined to generate a liklihood ratio. Calculation of a patient, risk is the product of liklihood ratio and age-related risk. Risk assessment is weight for maternal age. The median values of aFP, hCG and uE{sub 3} were well correlated with gestational age, respectively (r=0.94, p=0.003; r=-0.97, p=0.029; r=0.99, p<0.001, respectively). The median value of hCG were correlated withmateral age (r=0.13, p=0.04) but those of aFP and eU{sub 3} weren't (r=-0.17, p=0.22; r=0.36, p=0.09, respectively). The values of aFP, CG and uE{sub 3} between pregnancy younger than 35 years-old (n=87) and older than that (n=1640) were 51.67{+-}27.44, vs 54.65{+-}126.36, 46.45{+-}30.08 vs 51.33{+-}38.50 and 8.01{+-}11.01 vs 6.68{+-}7.23, respectively but all of them failed to show significant differences. A second-trimester risk for trisomy 21 > or = 1:270 was considered screen positive. Patients were screen positive for trisomy 21 if aFP < or 0.7 multiples of median (MOM), hCG> or 2.1 MOM and E{sub 3} < or 0.7 MOM. Patients were screen positive for neural tube defect if aFP >2.5 MOM. The initial screen-positive rate for both Down' syndrome and neural tube defect were 1.46% (26/1767); 0.73% (13/1767) with each other

  18. ALPHA-FETOPROTEIN IN FETAL SERUM, AMNIOTIC-FLUID, AND MATERNAL SERUM

    NARCIS (Netherlands)

    VANLITH, JMM; BEEKHUIS, [No Value; VANLOON, AJ; MANTINGH, A; DEWOLF, BTHM; BREED, ASPM

    1991-01-01

    In order to gain more insight into the association between alpha-fetoprotein (AFP) and fetal chromosomal disorders, especially Down's syndrome, we measured AFP in fetal serum, amniotic fluid, and maternal serum at cordocentesis. We compared the concentration and gradient of AFP in these three compar

  19. The Value of Combined Serum Alpha-fetoprotein Heteroplasmon-L3 Concentration and AFP-L3/Alpha-fetoprotein Ratio Detection in the Early Diagnosis of Primary Liver Cancer%联合检测血清甲胎蛋白异质体浓度与甲胎蛋白异质体百分含量在原发性肝癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    黄彩云; 韩素桂; 贾红莲

    2013-01-01

    Objective To explore the value of combined serum alpha-fetoprotein inform (AFP-L3) and AFP-L3/AFP as biomarkers in the early diagnosis of hepatocellular carcinoma (HCC).Methods A total of 137 patients from the People's Hospital of Tangshan of Hebei Union University were included in our study.They were treated in hospital from March to November 201 1.All the patients were at the age of 28 to 77 years old,and 98 of them were men,and 39 were women.According to the pathological diagnosis,patients were divided into two groups.HCC group had 92 patients,and benign liver disease group had 45 patients including 37 with liver cirrhosis and 8 with chronic hepatitis.ELISA method was used to test the serum AFP-L3 level.At the same time,microcentrifugal column method was adopted to separate AFP-L3 from the patients' serum,and electrochemical luminescence method was used to detect the separated AFP-L3 and total AFP levels.Then,we calculated the ratio of AFP-L3/AFP.We also calculated the statistical indexes of AFP-L3 level and AFP-L3/AFP ratio in the diagnosis of HCC,such as sensitivity,specificity and Youden index.The above indexes of combined AFP-L3 level and AFP-L3/AFP ratio were also detected to evaluated its diagnostic value.Results HCC group AFP-L3 level (109.04 ± 62.51) ng/mL was significantly higher than that of the benign liver disease group [(25.96 ± 49.43) ng/mL; t=8.28,P < 0.001].HCC group serum AFP-L3/AFP ratio (17.35% ± 14.48%) was significantly higher than that of the benign liver disease group (5.62% ± 6.38%,t=6.545,P < 0.000 1).With serum AFP-L3 level > 38.0 ng/mL as a critical value of primary liver cancer diagnosis,the sensitivity was 83.69%,and the specificity was 88.88%.For AFP-L3/AFP ratio > 10% as a critical value in primary liver cancer diagnosis,the sensitivity was 83.69%,and the specificity was 95.55%.When serum AFP-L3 level > 38.0 ng/mL and AFP-L3/AFP ratio > 7.5% were combined in primary liver cancer diagnosis

  20. Anxiety in women with low maternal serum alpha-fetoprotein screening results.

    Science.gov (United States)

    Abuelo, D N; Hopmann, M R; Barsel-Bowers, G; Goldstein, A

    1991-06-01

    The purpose of this study was to measure anxiety in pregnant women who had low maternal serum alpha-fetoprotein (MSAFP) screening test levels, received genetic counselling and chose to undergo amniocentesis for fetal chromosome analysis. Their anxiety levels were compared with the levels in women undergoing amniocentesis because of advanced maternal age. The results indicate a higher level of anxiety in women with low alpha-fetoprotein (AFP) levels.

  1. Combined serum hepatoma-speciifc alpha-fetoprotein and circulating alpha-fetoprotein-mRNA in diagnosis of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Wei Wu; Deng-Fu Yao; Yong-Mei Yuan; Ji-Wei Fan; Xiu-Feng Lu; Xiao-Hua Li; Li-Wei Qiu; Lei Zong; Xin-Hua Wu

    2006-01-01

    BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors worldwide, its prognosis is poor, and early detection is of utmost importance. Although alpha-fetoprotein (AFP) is a useful marker for detecting and monitoring HCC development, the false-negative or false-positive rates with AFP alone may be as high as 30%-40%for patients with small HCCs. To enhance the speciifcity and accuracy of AFP measurements for HCC, we analyzed AFP expression states in livers, detected the hepatoma-speciifc AFP (HS-AFP) fraction and AFP-mRNA from peripheral blood mononuclear cells, and explored their clinical implications for HCC diagnosis. METHODS:AFP expression and hepatocyte distributions in liver specimens were investigated by an immunohistoche-mical assay. Total RNAs were extracted from circulating blood, synthesized to cDNA through random primers and reverse transcriptase, and fragments of the AFP gene were ampliifed by a nested-PCR assay. The HS-AFP fraction was separated by lectin-afifnity chromatography and its level was detected by radioimmunoassay. RESULTS: The incidence of AFP was 73.3% in HCC tissues and its expression in HCCs with moderate or low differentiation was signiifcantly stronger than that of HCCs with high differentiation (P CONCLUSIONS: Different AFP expression is present in different parts of HCC tissues. HS-AFP and AFP-mRNA fragments improve sensitivity and speciifcity, and both are useful markers to diagnose HCC or monitor metastasis and relapse.

  2. FIRST-TRIMESTER MATERNAL SERUM ALPHA-FETOPROTEIN AS A MARKER FOR FETAL CHROMOSOMAL DISORDERS

    NARCIS (Netherlands)

    VANLITH, JMM

    1994-01-01

    We evaluated first-trimester maternal serum alpha-fetoprotein (MS-AFP) as a marker for fetal chromosomal disorders. The multicentre study was performed under the auspices of the Dutch Working Party on Prenatal Diagnosis. MS-AFP was measured in 2404 normal pregnancies and 72 chromosomally abnormal pr

  3. Alpha fetoprotein antagonizes apoptosis induced by paclitaxel in hepatoma cells in vitro.

    Science.gov (United States)

    Zhu, Mingyue; Li, Wei; Lu, Yan; Dong, Xu; Chen, Yi; Lin, Bo; Xie, Xieju; Guo, Junli; Li, Mengsen

    2016-01-01

    Hepatocellular carcinoma (HCC) cell resistance to the effects of paclitaxel has not been adequately addressed. In this study, we found that paclitaxel significantly inhibited the viability of HLE, Bel 7402 and L-02 cells in a dose- and time-dependent manner. HLE cells and L-02 cells resisted the cytotoxicity of paclitaxel when transfected with pcDNA3.1-afp vectors. However, Bel 7402 cell sensitivity to paclitaxel was increased when transfected with alpha fetoprotein (AFP)-siRNA. Bel 7402 cell resistance to paclitaxel was associated with the expression of the "stemness" markers CD44 and CD133. Paclitaxel significantly inhibited growth and promoted apoptosis in HLE cells and L-02 cells by inducing fragmentation of caspase-3 and inhibiting the expression of Ras and Survivin, but pcDNA3.1-afp vectors prevented these effects. However, paclitaxel could not significantly promote the cleavage of caspase-3 or suppress the expression of Ras and Survivin in Bel 7402 cells. Silenced expression of AFP may be synergistic with paclitaxel to restrain proliferation and induce apoptosis, enhance cleavage of caspase-3, and suppress the expression of Ras and Survivin. Taken together, AFP may be an important molecule acting against paclitaxel-inhibited proliferation and induced apoptosis in HCC cells via repressing the activity of caspase-3 and stimulating the expression of Ras and Survivin. Targeted inhibition of AFP expression after treatment with paclitaxel is an available strategy for the therapy of patients with HCC. PMID:27255186

  4. CONCANAVALIN-A VARIANTS OF ALPHA-FETOPROTEIN IN FIRST TRIMESTER FETUSES WITH TRISOMY-21 AND WITH NORMAL KARYOTYPES

    NARCIS (Netherlands)

    LOS, FJ; JANSE, HC; BRANDENBURG, H; DEVRIJ, RW; DEBRUIJN, HWA

    1995-01-01

    Total alpha-fetoprotein (AFP) concentrations and proportions of AFP non-reactive with the lectin concanavalin A (Con A) were studied in extracellular fluid of 22 first-trimester fetuses. Total AFP concentrations were significantly lower in fetuses with Down's syndrome than in those with Mendelian-in

  5. Electrophoresis and electro-affinity transfer with specific antibodies to alpha-fetoprotein for detection of circulating immune complexes of alpha-fetoprotein.

    Directory of Open Access Journals (Sweden)

    Taketa,Kazuhisa

    1984-08-01

    Full Text Available A combination of agarose gel electrophoresis and a newly developed technique of electro-affinity transfer was applied to the detection of circulating immune complexes of human alpha-fetoprotein (AFP and anti-AFP. After electrophoretic transfer to nitrocellulose membrane, to which affinity-purified polyclonal horse antibodies to human AFP were bound, the membranes were treated with or without rabbit immunoglobulins to human AFP, followed by overlaying with horseradish peroxidase-labeled goat anti-rabbit IgG for color development. Artificial complexes formed in vitro from human AFP and rabbit anti-AFP were clearly separated from free AFP by the agarose electrophoresis. The complexes were stained 20-40% as dark as the equivalent amount of free AFP by treatment with rabbit anti-AFP, and 10-20% as dark without the antibody treatment over a wide range of antigen-antibody ratios.

  6. Autism Spectrum Disorders and Maternal Serum alpha-Fetoprotein Levels During Pregnancy

    DEFF Research Database (Denmark)

    Abdallah, Morsi; Grove, Jakob; Hougaard, David M;

    2011-01-01

    Objective: Numerous studies have been trying to disentangle the complex pathophysiology of autism spectrum disorders (ASD). In our study, we explored the potential role of maternal serum (MS) alpha-fetoprotein (AFP) in the prediction and the pathophysiology of ASD. Methods: A total of 112 patients...

  7. Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility

    OpenAIRE

    Gabant, Philippe; Forrester, Lesley; Nichols, Jennifer; Van Reeth, Thierry; De Mees, Christelle; Pajack, Bernard; Watt, Alistair; Smitz, Johan; Alexandre, Henri; Szpirer, Claude; Szpirer, Josiane

    2002-01-01

    The alpha-fetoprotein gene (Afp) is a member of a multigenic family that comprises the related genes encoding albumin, alpha-albumin, and vitamin D binding protein. The biological role of this major embryonic serum protein is unknown although numerous speculations have been made. We have used gene targeting to show that AFP is not required for embryonic development. AFP null embryos develop normally, and individually transplanted homozygous embryos can develop in an AFP-deficient microenviron...

  8. Maternal serum alpha-fetoprotein and fetal triploidy.

    Science.gov (United States)

    Pircon, R A; Towers, C V; Porto, M; Gocke, S E; Garite, T J

    1989-10-01

    Fetal triploidy is commonly found in early pregnancy. The majority of these pregnancies spontaneously abort in the first trimester. Occasionally, the pregnancy progresses to the second and third trimesters. We reviewed the maternal serum alpha-fetoprotein (MSAFP), amniotic fluid alpha-fetoprotein (AFP), amniotic fluid acetylcholinesterase (ACHE), fetal pathology, and placental pathology in sex second-trimester pregnancies complicated by fetal triploidy. Four of these patients had MSAFP values greater than 7.5 multiples of the median (MoM). Five of six pregnancies had MSAFP values greater than 2.25 MoM. All five of these patients had a partial mole. Four patients had amniotic fluid AFP values greater than 2.0 MoM. Two fetuses had associated neural tube defects. These were the only patients with positive amniotic fluid ACHE. None of the other patients had fetuses with anomalies that are known to be associated with an elevated MSAFP. The elevated MSAFP appeared to be related to the presence of a partial mole. Two of the five cases with an MSAFP greater than 2.25 MoM did not have sonographic evidence of a significant anomaly. Therefore, karyotyping can be of benefit in evaluating patients with elevated MSAFP. PMID:2480590

  9. AFP — EDRN Public Portal

    Science.gov (United States)

    AFP is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin.

  10. Alpha-fetoprotein-producing rectum adenocarcinoma:A case report

    Institute of Scientific and Technical Information of China (English)

    Kai Qin; Qiang Fu; Renliang Wu; Xianglin Yuan

    2013-01-01

    A case with an Alpha-fetoprotein-producing (AFP-producing) carcinoma originating from the rectum was de-scribed. A 41-year-old man, who underwent a pal iative surgery for rectal carcinoma, was diagnosed with occupying liver lesions and a remarkable AFP elevation (3484.61 ng/mL), and the AFP declined obviously 10 days after the pal iative surgery. So we carried out a biopsy of the liver lesions. The histopathology was reported as low dif erentiation adenocarcinoma. The immunohistochemistry of the tumor cells via liver biopsy showed:Vil in, CDX-2 was positive, Glypican-3 was partial positive, CK7, CK20, AFP, Hepatocyte were al negative. The initial histopathology was reported as an AFP-producing rectum adeno-carcinoma with liver metastasis, which was a rare disease. So far, only 17 reports, none has been reported in China. Then, we summarize the characteristic of the disease:diagnosed with hepatic metastasis, raised serum AFP and a poor outcome, in addition to primary symptoms. This kind of disease is highly malignant.

  11. Alpha-fetoprotein-producing primary lung carcinoma: A case report

    Directory of Open Access Journals (Sweden)

    Tokusashi Yoshihiko

    2011-05-01

    Full Text Available Abstract Alpha-fetoprotein (AFP-producing lung adenocarcinoma is a rare type of lung cancer, with its characteristics not yet fully clarified. We recently encountered a case of this type of lung cancer. The patient was a 69-year-old man who consulted an internist with the chief complaint of epigastric pain. Chest X-ray and CT revealed a lobulated mass measuring 70 mm in diameter in the right lower lung field and a metastasis in the right hilar lymph nodes. Of the tumor markers, the serum AFP was elevated (4620 ng/ml, and the serum carcinoembryonic antigen and carbohydrate antigen 19-9 were also slightly elevated. Transbronchial lung biopsy revealed the diagnosis of lung cancer. Under thoracoscopic assistance, right lower lobectomy + mediastinal lymph node dissection was carried out. Immunostaining showed the tumor cells to be AFP-positive. The tumor was thus diagnosed as an AFP-producing lung adenocarcinoma. The patient followed an uneventful clinical course after the surgery, with serum AFP decreasing to the normal range by about 2 weeks after the surgery. As of this writing, no sign of tumor recurrence has been noted. This case is presented here with a review of the literature.

  12. Female Mice Deficient in Alpha-Fetoprotein Show Female-Typical Neural Responses to Conspecific-Derived Pheromones

    OpenAIRE

    Olivier Brock; Matthieu Keller; Quentin Douhard; Julie Bakker

    2012-01-01

    The neural mechanisms controlling sexual behavior are sexually differentiated by the perinatal actions of sex steroid hormones. We recently observed using female mice deficient in alpha-fetoprotein (AFP-KO) and which lack the protective actions of AFP against maternal estradiol, that exposure to prenatal estradiol completely defeminized the potential to show lordosis behavior in adulthood. Furthermore, AFP-KO females failed to show any male-directed mate preferences following treatment with e...

  13. Telomerase Inhibition Decreases Alpha-Fetoprotein Expression and Secretion by Hepatocellular Carcinoma Cell Lines: In Vitro and In Vivo Study

    OpenAIRE

    Roula Tahtouh; Anne-Sophie Azzi; Nada Alaaeddine; Soulaima Chamat; Hasnaa Bouharoun-Tayoun; Layal Wardi; Issam Raad; Riad Sarkis; Najibe Abou Antoun; George Hilal

    2015-01-01

    Alpha-fetoprotein (AFP) is a diagnostic marker for hepatocellular carcinoma (HCC). A direct relationship between poor prognosis and the concentration of serum AFP has been observed. Telomerase, an enzyme that stabilizes the telomere length, is expressed by 90% of HCC. The aim of this study was to investigate the effect of telomerase inhibition on AFP secretion and the involvement of the PI3K/Akt/mTOR signaling pathway. Proliferation and viability tests were performed using tetrazolium salt. A...

  14. Prussian blue modified amperometric FIA biosensor: one-step immunoassay for alpha-fetoprotein.

    Science.gov (United States)

    Guan, Jian-Guo; Miao, Yu-Qing; Chen, Jian-Rong

    2004-03-15

    The aim of this study was to develop a rapid method to measure alpha-fetoprotein (AFP) in human serum by use of one-step sandwich enzyme-linked immunosorbent assay (ELISA)-based immunobiosensor with disposable screen-printed carbon electrode technology. Prussian blue (PB) was deposited using cyclic voltammetry (CV) on the surface of electrode to catalyze H202 from the reaction of glucose oxidase. It took only about 30 min to complete the whole experimental procedure through flow injection analysis (FIA). A detection range obtained is in the range from 5 to 500 ng/ml AFP. This detection seems to be quick, reliable and practical. PMID:15128097

  15. Alpha fetoprotein antagonizes apoptosis induced by paclitaxel in hepatoma cells in vitro

    OpenAIRE

    Mingyue Zhu; Wei Li; Yan Lu; Xu Dong; Yi Chen; Bo Lin; Xieju Xie; Junli Guo; Mengsen Li

    2016-01-01

    Hepatocellular carcinoma (HCC) cell resistance to the effects of paclitaxel has not been adequately addressed. In this study, we found that paclitaxel significantly inhibited the viability of HLE, Bel 7402 and L-02 cells in a dose- and time-dependent manner. HLE cells and L-02 cells resisted the cytotoxicity of paclitaxel when transfected with pcDNA3.1-afp vectors. However, Bel 7402 cell sensitivity to paclitaxel was increased when transfected with alpha fetoprotein (AFP)-siRNA. Bel 7402 cell...

  16. The immunosuppression role of alpha-fetoprotein in human hepatocellular carcinoma.

    Science.gov (United States)

    Meng, Wenbo; Bai, Bing; Bai, Zhongtian; Li, Yan; Yue, Ping; Li, Xun; Qiao, Liang

    2016-06-01

    Human hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death. Alpha-fetoprotein (AFP) is perhaps the best-defined tumor marker for HCC, and as such, it is widely used in clinical settings as an adjuvant diagnostic and prognostic tool. Up to 70% of HCC cases exhibit elevated serum level of AFP, but its pathophysiological functions in HCC are poorly defined. It is now known that AFP is not just a fetal form of carrier protein and a tumor marker, it is also critically involved in the regulation of several important cellular functions, such as cell growth, differentiation, apoptosis, angiogenesis, and immune regulation. In this mini-review, we summarize the recent development of AFP in hepatocellular carcinoma. PMID:27448785

  17. Alpha-fetoprotein expression is a potential prognostic marker in hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Dénes G(o)r(o)g; János Reg(o)ly-Mérei; Sándor Paku; László Kopper; Péter Nagy

    2005-01-01

    AIM: To characterize the alpha-fetoprotein (AFP) positive and negative hepatocellular carcinoma (HCC) samples.METHODS: Thirty-seven paraffin-embedded human HCC samples were analyzed by immunohistochemistry for the following antigens: AFP, β-catenin, p53, CD44, MSH-2,MLH-1, and HNF-4. The tumors were divided into two groups based on the AFP expression. The immunophenotypic data and important clinical parameters were studied between the two groups.RESULTS: Twenty-one of the thirty-seven examined HCCs were AFP positive. Seven with nuclear p53 staining were AFP positive, while seven tumors with nuclear β-catenin staining were AFP negative. CD44 staining and high histological tumor grade were more frequent among the AFP-positive HCCs. The other immunophenotypical and dinical parameters did not show statistically significant difference in their distribution between the AFP positive and negative samples.CONCLUSION: AFP expression in HCC correlates with unfavorable prognostic factors, while nuclear β-catenin positivity is more common among the AFP-negative liver tumors. This observation supports the microarray data onin vivo human tumors.

  18. Clinical Value of Hepatoma-Specific Alpha-Fetoprotein in the Diagnosis of Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    Runzhou Ni; Mingbing Xiao; Fei Jin; Cuihua Lu; Jiefei Huang; Xianyong Meng

    2006-01-01

    OBJECTIVE To study the clinical value of hepatoma-specific alpha-fetoprotein (HS-AFP) in the diagnosis and differential diagnosis of hepatocellular carcinoma (HCC).METHODS A method of vertical slab polyacrylamide gel electrophoresis with discontinuous buffer system was developed to separate AFP subtypes. After separation, the AFP subtypes were transferred to nitrocellulose and reacted first with rabbit anti-human AFP and then with goat anti-rabbit IgG-HRP. Finally, AFP subtypes were visualized by reacting with 3,3'-diaminobenzidine tetrahydrochloride. A HS-AFP band was determined in 82 cases with HCC and 95 cases with benign liver diseases.The correlations between the positive rates of HS-AFP and serum AFP concentration, tumor size as well as portal vein metastasis were analyzed.RESULTS Serum AFP in the cases with various liver diseases was separated into one to several bands. The fastest band on electrophoresis (FAFP) was found in all patients, while the band at the cathodal site (HSAFP) was detected predominantly in HCC but rarely in benign liver diseases. The positive rate of HS-AFP in HCC was 74.4%, which was significantly higher than that in benign liver diseases (9.1%, 7.3% and 10.0% in liver cirrhosis, chronic hepatitis and acute hepatitis respectively). HSAFP was detected in 3 out of 9 HCC cases with AFP<50 μg/L , but in none of 22 cases of benign liver diseases with the same AFP concentration. HS-AFP correlated with serum AFP concentration and tumor size to some extent, but not with portal vein metastasis.CONCLUSION HS-AFP increases the sensitivity of diagnosing HCC in patients with negative AFP, and is useful in distinguishing high AFP due to HCC from that caused by benign liver diseases.

  19. Alpha-fetoprotein-L3 and Golgi protein 73 may serve as candidate biomarkers for diagnosing alpha-fetoprotein-negative hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    Zhang ZG

    2015-12-01

    Full Text Available Zhiguo Zhang,1 Yanying Zhang,2 Yeying Wang,1 Lingling Xu,3 Wanju Xu3 1Department of Clinical Laboratory, Zhangqiu Maternity and Child Care Hospital, Zhangqiu, 2Department of Clinical Laboratory, Zaozhuang City Wangkai Infection Hospital, Zaozhuang, 3Department of Clinical Laboratory, Qianfoshan Hospital, Jinan, People’s Republic of China Abstract: Currently, there is no reliable biomarker for use in diagnosing alpha-fetoprotein (AFP-negative hepatocellular carcinoma (HCC. Such a biomarker would aid in making an early diagnosis of AFP-negative HCC, ensuring the timely initiation of treatment. This study examined AFP-L3 and Golgi protein 73 (GP73 as candidate biomarkers for AFP-negative HCC. The affinity adsorption method and enzyme-linked immunoassays were separately used to determine serum levels of AFP-L3 and GP73 in 50 patients with AFP-negative HCC, 30 non-HCC patients, and 50 healthy subjects. Fifty percent of patients with AFP-negative HCC tested positive for AFP-L3, while 3.33% of non-HCC patients and 2.00% of healthy subjects were AFP-L3 positive. Patients with AFP-negative HCC had significantly higher serum levels of AFP-L3 compared to non-HCC patients and healthy individuals; however, there was no significant difference in the AFP-L3 levels of non-HCC patients and healthy subjects. Sixty-six percent of patients with AFP-negative HCC tested positive for GP73, while 10% of non-HCC patients and 0% of healthy subjects were GP73-positive. Patients with AFP-negative HCC had significantly higher serum levels of GP73 compared to non-HCC patients and healthy subjects, but there was no significant difference between the GP73 levels of non-HCC patients and healthy individuals. Moreover, 20 patients with AFP-negative HCC were both AFP-L3- and GP73-positive, while no non-HCC patients or healthy subjects tested positive for both markers. Either AFP-L3 or GP73 may be used as a biomarker for diagnosing AFP-negative HCC, while their combined use

  20. Developmental alterations in the alpha-fetoprotein sugar chain in maternal serum analyzed by lectin affinity electrophoresis.

    Directory of Open Access Journals (Sweden)

    Kawahara N

    1999-06-01

    Full Text Available Our purpose was to investigate developmental alterations of human alpha-fetoprotein (AFP oligosaccharides in maternal serum by lectin affinity electrophoresis and to compare the AFP glycoforms in maternal serum with those in umbilical cord serum and amniotic fluid. AFP glycoforms were separated by affinity electrophoresis with concanavalin A (Con A, lentil lectin (LCA, erythroagglutinating phytohemagglutinin (E-PHA and Allomyrina dichotoma lectin (allo A and detected by sensitive antibody affinity blotting. In maternal serum, increased proportions of Con A-nonreactive AFP (AFP-C1, LCA strongly-reactive AFP (AFP-L3 and E-PHA-reactive AFP (AFP-P4 and AFP-P5 decreased gradually during the early gestational weeks. Allo A-nonreactive AFP (AFP-A1 and asialo-AFP were found only in amniotic fluids during early gestational weeks. The percentages of these glycoforms at full term were almost the same among those body fluids. Since the glycoforms of maternal serum AFP were close to those of umbilical cord serum AFP, lectin-affinity electrophoretic analysis of maternal serum AFP may be useful for evaluating the developmental state of fetus by examining the nature of AFP sugar chain.

  1. Characterization of Alpha-fetoprotein Levels in Three Dolphin Species: Development of Sensitive Immunoassays for Analysis of the Pregnancy-associated Variations

    OpenAIRE

    Morita, Yuka; Hiramatsu, Naoshi; Fujita, Toshiaki; AMANO, Haruna; KATSUMATA, Etsuko; Arai, Kazutoshi; IWASAKI, Toshihide; Todo, Takashi; HARA, Akihiko

    2013-01-01

    Abstract A single radial immunodiffusion (SRID) assay and a chemiluminescent immunoassay (CLIA) were initially developed for alpha-fetoprotein (AFP) of the striped dolphin. Utilizing these developed assays, we investigated pregnancy-associated changes in the levels of AFP in the sera of fetuses and pregnant females of three dolphin species; samples were either collected from captive individuals or obtained as fishery by-products. The concentrations of AFP in the fetal serum ranged from 419.0 ...

  2. Application value of joint detection of alpha-fetoprotein(AFP) variant and AFP in early diagnosis of primary hepato-cellular carcinoma%甲胎蛋白异质体和甲胎蛋白联合检测的临床价值

    Institute of Scientific and Technical Information of China (English)

    王国芳; 徐美珍; 张欢妍; 唐国建

    2015-01-01

    目的:开展甲胎蛋白异质体和甲胎蛋白联合检测,以早期发现和诊断肝细胞癌( HCC),鉴别良恶性肝脏肿瘤。方法检测肝细胞癌( HCC)30例,慢性肝病78例患者血清中AFP-L3和AFP的含量,血清 AFP-L3分离检测采用亲和微量离心纯化柱法,血清中获取的AFP-L3,AFP采用微粒子化学发光免疫法检测,计算AFP-L3占AFP的比率即AFP-L3%。结果30例HCC患者AFP的值为8.8~1524.1 ng/ml,其中AFP-L3%的阳性率为80%,而78例慢性肝病患者中AFP的值为3.2~580.1 ng/ml,AFP-L3%的阳性率为14.1%。 HCC组AFP异质体的阳性率与慢性肝病组有显著差异(P﹤0.01)。结论联合检测甲胎蛋白异质体和甲胎蛋白对HCC,慢性肝炎和肝硬化的患者鉴别诊断,有着重要的临床价值。%Objective To early detect and diagnose hepatocellular carcinoma ( HCC) and to identify benign and malignant tumors of liver with joint detection of AFP variant and AFP .Methods AFP-L3 and AFP content in serum of 30 cases of HCC and 78 cases of chronic liver disease were detected .Affinity micro centrifugal purification column was used to separate and de-termine serum AFP-13.Level of AFP-L3 and AFP extracted from the serum was detected by a chemiluminescence immunoassay method using microparticle .The ratio between AFP-L3 and AFP was calculated as AFP-L3%.Results In the 30 patients of HCC,the level of AFP fluctuated between 8.8 ng/ml and 1524.1 ng/ml,among which the positive rate of AFP-L3 was 80%. Whereas in the 78 patients of chronic liver disease ,the level of AFP fluctuated between 3.2 ng/ml and 580.1 ng/ml,among which the positive rate of AFP-L3 was 14.1%.Conclusion The positive rate of AFP variant in HCC group and chronic liver disease group shows significant difference (P﹤0.01).Joint detection of AFP variant and AFP is very important in differential diagnosis of patients with HCC ,chronic hepatitis and cirrhosis .Therefore

  3. Elevated serum alpha fetoprotein levels promote pathological progression of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Peng Li; Shan-Shan Wang; Hui Liu; Ning Li; Michael A McNutt; Gang Li; Hui-Guo Ding

    2011-01-01

    AIM: To investigate the biological role of alpha fetoprotein (AFP) and its clinical significance in carcinogenesis of hepatocellular carcinoma (HCC).METHODS: Clinical analysis of HCC patients and immunohistochemical examination were conducted to eva-luate the relationship between serum AFP level and patient mortality. Confocal microscopy, Western blotting, dimethylthiahzolyl-2,5-diphenyl-tetrazolium bromide, Cell Counting Kit-8 assays and flow cytometry were performed to explore the possible mechanism.RESULTS: Among the 160 HCC patients enrolled in this study, 130 patients survived 2 years (81.25%), with a survival rate of 86.8% in AFP 250 μg/L group, demonstrating a higher mortality rate in HCC patients with higher AFP levels. Surgical treatment was beneficial only in patients with low AFP levels. The mortality rate of HCC patients with high AFP levels who were treated surgically was apparently higher than those treated with conservative management. The results of immunohistochemistry showed that AFP and AFP receptor were merely expressed in tissues of HCC patients with positive serum AFP. Consistently, in vitro analysis showed that AFP and AFPS were expressed in HepG2 but not in HLE cells. AFP showed a capability to promote cell growth, and this was more apparent in HepG2 cells, in which the proliferation was increased by 3.5 folds. Cell cycle analysis showed that the percentage of HepG2 cells in S phase after exposure to AFP was modestly increased.CONCLUSION: HCC patients with higher AFP levels show a higher mortality rate, which appears to be attributable to the growth promoting properties of AFP.

  4. Diagnostic value of alpha fetoprotein (AFP),α-L-fucosidase(AFU) and carbohydrate antigen 199 (CA199)combined detection in early stage orimary hepatocellular carcinoma (PHC)%AFP、AFU、CA199联合检测对早期原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    邓爱红; 彭燕华; 周少雄; 沈永坚; 肖海萍; 何彩云; 林经良

    2015-01-01

    目的:探讨肿瘤标志物甲胎蛋白(AFP)、α-L-岩藻糖苷酶(AFU)、糖类抗原199(CA199)对早期原发性肝癌(primary carcinoma of the liver,PHC)联合检测的诊断价值。方法:选取2013年1月到2014年5月佛山市第二人民医院肝科门诊初诊患者,通过病史、症状和查体拟诊为疑似PHC患者,再采用诊断金标准将疑似患者(研究对象)确诊分为PHC(48例)和非PHC(76例),所有研究对象均检测血清AFP、AFU和CA199,检测AFP和CA199采用化学发光法,检测AFU采用全自动生化仪器法,采用循证检验医学的方法统计各诊断价值性能指标。结果:PHC组患者AFP、AFU和CA199水平较非PHC组非常显著升高(P<0.01)。单一指标AFP、AFU和CA199检测PHC的诊断灵敏度分别为74.3%、51.4%、51.4%。单一指标AFP、AFU和CA199检测PHC的诊断特异性分别为82.4%、94.1%、94.1%。3指标并联灵敏度最高,为86.4%,其漏检率为13.6%。3指标串联和AFU/CA199串联特异性最高,约为96.5%,其误诊率为3.5%。结论:3指标并联是最佳PHC筛查联合指标,AFU和CA199串联是最佳PHC确诊联合指标。%Objective:To investigate the diagnostic value of the alpha fetoprotein (AFP), α-L- fucosidase(AFU), carbohydrate antigen 199 (CA199) and their combinations in Early Stage Primary Hepatocellular Carcinoma (PHC). Methods:Selected the Hepatological outpatients at their first visit to the second people’s hospital of Foshan from January,2013 to May,2014.Suspicious PHC patients were diagnosed by medical history, symptoms and physical check,and suspicious PHC patients were classified into PHC patients(n=48) and non-PHC patients(n=76) following the diagnosed gold criteria.The concentration of AFP and CA199 was determined by Chemiluminescence method.The concentration of AFU were determined by full automatic biochemical instrument.The assessed parameters of diagnostic value were measured using the evidence-based laboratory

  5. Serum alpha-fetoprotein response can predict prognosis in hepatocellular carcinoma patients undergoing radiofrequency ablation therapy

    International Nuclear Information System (INIS)

    Aims: To evaluate the clinical inference of serum alpha-fetoprotein (AFP) response in hepatocellular carcinoma (HCC) patients undergoing percutaneous radiofrequency ablation (RFA). Materials and methods: Three hundred and thirteen previously untreated HCC patients were enrolled in the study. The optimal AFP response was defined as >20% decrease from baseline after 1 month of RFA for those with a baseline AFP level of ≥100 ng/ml. The impact of AFP response on prognosis was analysed and prognostic factors were assessed. Results: After a median follow-up of 26.7 ± 19.1 months, 49 patients died and 264 patients were alive. The cumulative 5 year survival rates were 75.3 and 57.4% in patients with an initial AFP of 1.1 (p = 0.009), non-optimal AFP response (p = 0.023), and creatinine >1.5 mg/dl (p = 0.021) were independent risk factors predictive of poor overall survival. Besides, the cumulative 5 year recurrence rates were 83.4 and 100% in optimal and non-optimal AFP responders, respectively (p 5/mm3 (p = 0.048), tumour size >2 cm (p = 0.027), and non-optimal AFP response (p < 0.001) were independent risk factors associated with tumour recurrence after RFA. Conclusions: Serum AFP response may be a useful marker for predicting prognosis in HCC patients undergoing RFA.

  6. User acceptability of an alpha-fetoprotein screening programme

    DEFF Research Database (Denmark)

    Jørgensen, Finn Stener

    1995-01-01

    The objective of the study was to determine user acceptability among women who were classified as false positives or test negatives in an alpha-fetoprotein screening programme. The study was performed as a questionnaire study over a one-year period from October 1, 1988 to September 30, 1989 at...... Hvidovre University Hospital, Copenhagen, and the county hospitals of Sønderjylland, Denmark. The participating subjects were 4104 pregnant women who had had an alpha-fetoprotein test and had completed 30 weeks of gestation, when the questionnaire was delivered. Main outcome measures were degree and...... duration of anxiety, influence on daily life and whether the woman wanted the alpha-fetoprotein test again in a new pregnancy. Three thousand, three hundred and thirty-one questionnaires were analyzed. The participation rate was 81.2%. For 219 women (6.6%), the first alpha-fetoprotein test was abnormal...

  7. The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    AIM The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of 3H-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722×10-9M (Bmax=12810 sites per cell) and 8.931× 10-SM (Bmax=l19700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene.

  8. Fabrication of protein microarrays for alpha fetoprotein detection by using a rapid photo-immobilization process

    Directory of Open Access Journals (Sweden)

    Sirasa Yodmongkol

    2016-03-01

    Full Text Available In this study, protein microarrays based on sandwich immunoassays are generated to quantify the amount of alpha fetoprotein (AFP in blood serum. For chip generation a mixture of capture antibody and a photoactive copolymer consisting of N,N-dimethylacrylamide (DMAA, methacryloyloxy benzophenone (MaBP, and Na-4-styrenesulfonate (SSNa was spotted onto unmodified polymethyl methacrylate (PMMA substrates. Subsequently to printing of the microarray, the polymer and protein were photochemically cross-linked and the forming, biofunctionalized hydrogels simultaneously bound to the chip surface by short UV- irradiation. The obtained biochip was incubated with AFP antigen, followed by biotinylated AFP antibody and streptavidin-Cy5 and the fluorescence signal read-out. The developed microarray biochip covers the range of AFP in serum samples such as maternal serum in the range of 5 and 100 ng/ml. The chip production process is based on a fast and simple immobilization process, which can be applied to conventional plastic surfaces. Therefore, this protein microarray production process is a promising method to fabricate biochips for AFP screening processes.

  9. Serial Changes in Alpha-Fetoprotein Levels During Therapy for Chronic Hepatitis C

    Directory of Open Access Journals (Sweden)

    Altug Senol

    2014-12-01

    Full Text Available Aim: Alpha-fetoprotein (AFP has been widely used as a diagnostic marker for hepatocellular carcinoma. Some patients with hepatitis C show high AFP values, but no evidence of hepatocellular carcinoma. The aim of this study is to assess the influence of antiviral treatment on the serum AFP in patients with chronic hepatitis C without hepatocellular carcinoma. Material and Method: Thirty seven chronic hepatitis C patients (20 females and 17 males were included in the study. All patients were given a combined treatment of pegylated or conventional interferon (IFN and ribavirin. Serum AFP was measured at baseline and on months 3-6-12 of the therapy. Results: Compared to the pretreatment levels of ALT (88,59 ± 57,22 IU, those at 3, 6 and 12 months were statistically lower (p0,05, to 4,34 ± 4,64 (p>0,05 and to 2,63 ± 2,17 (p10 ng/ml. In these patients, mean serum AFP levels were decreased from pretreatment level of 15,09 ± 5,92 ng/ml to 11,39±3,30, to 6,97±2,53 (p

  10. Alpha-fetoprotein-targeted reporter gene expression imaging in hepatocellular carcinoma.

    Science.gov (United States)

    Kim, Kwang Il; Chung, Hye Kyung; Park, Ju Hui; Lee, Yong Jin; Kang, Joo Hyun

    2016-07-21

    Hepatocellular carcinoma (HCC) is one of the most common cancers in Eastern Asia, and its incidence is increasing globally. Numerous experimental models have been developed to better our understanding of the pathogenic mechanism of HCC and to evaluate novel therapeutic approaches. Molecular imaging is a convenient and up-to-date biomedical tool that enables the visualization, characterization and quantification of biologic processes in a living subject. Molecular imaging based on reporter gene expression, in particular, can elucidate tumor-specific events or processes by acquiring images of a reporter gene's expression driven by tumor-specific enhancers/promoters. In this review, we discuss the advantages and disadvantages of various experimental HCC mouse models and we present in vivo images of tumor-specific reporter gene expression driven by an alpha-fetoprotein (AFP) enhancer/promoter system in a mouse model of HCC. The current mouse models of HCC development are established by xenograft, carcinogen induction and genetic engineering, representing the spectrum of tumor-inducing factors and tumor locations. The imaging analysis approach of reporter genes driven by AFP enhancer/promoter is presented for these different HCC mouse models. Such molecular imaging can provide longitudinal information about carcinogenesis and tumor progression. We expect that clinical application of AFP-targeted reporter gene expression imaging systems will be useful for the detection of AFP-expressing HCC tumors and screening of increased/decreased AFP levels due to disease or drug treatment. PMID:27468205

  11. Contribution of alpha-fetoprotein in liver transplantation for hepatocellular carcinoma

    Science.gov (United States)

    Charrière, Bérénice; Maulat, Charlotte; Suc, Bertrand; Muscari, Fabrice

    2016-01-01

    Alpha-fetoprotein (AFP) is the main tumor biomarker available for the management of hepatocellular carcinoma (HCC). Although it is neither a good screening test nor an accurate diagnostic tool for HCC, it seems to be a possible prognostic marker. However, its contribution in liver transplantation for HCC has not been fully determined, although its use to predict recurrence after liver transplantation has been underlined by international societies. In an era of organ shortages, it could also have a key role in the selection of patients eligible for liver transplantation. Yet unanswered questions remain. First, the cut-off value of serum AFP above which liver transplantation should not be performed is still a subject of debate. We show that a concentration of 1000 ng/mL could be an exclusion criterion, whereas values of liver transplantation. These recent tools have associated AFP values with morphological criteria, thus refining pre-existing criteria, such as Milan, University of California, San Francisco, or “up-to-seven”. We provide a review of the different criteria submitted within the past years. Finally, AFP can be used to monitor recurrence after transplantation, although there is little evidence to support this claim. Future challenges will be to draft new international guidelines to implement the use of AFP as a selection tool, and to determine a clear cut-off value above which liver transplantation should not be performed.

  12. Alpha-fetoprotein-producing early gastric cancer of the remnant stomach: report of a case.

    Science.gov (United States)

    Shibata, Yutaka; Sato, Kouhei; Kodama, Masashi; Nanjyo, Hiroshi

    2007-01-01

    A 67-year-old man initially underwent a distal gastrectomy for early gastric cancer (T1, N0, M0; Stage IA) in March 1995. During the follow-up period, an elevation of the serum alpha-fetoprotein (AFP) level (98.8 ng/ml) and a liver tumor (S4) were detected. A left hepatectomy was performed in December 1996. Immunohistochemically, AFP-positive cells were present in both the primary gastric tumor and metastasized liver tumor. The serum AFP level normalized immediately, but it elevated again to 22.4 ng/ml. An endoscopic examination revealed a protruding lesion in the remnant stomach. A total resection of the remnant stomach was performed in February 2005. The tumor was evaluated T1, N0, M0; Stage IA, with positive staining for AFP. The patient has survived without any sign of recurrence for more than 11 years after the first diagnosis of cancer. To the best of our knowledge, this is the first case of a long-term survival of AFP-producing gastric cancer with successfully resected metachronous liver metastasis and gastric remnant carcinoma. PMID:17952534

  13. 甲胎蛋白异质体3及其比值对原发性肝癌和肝硬化的鉴别诊断价值研究%Value of Serum Alpha-fetoprotein Invariant AFP-L3 Assay in the Differential Diagnosis of Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    何建伟; 曹薇薇; 李静; 何建国; 程江

    2012-01-01

    Objective To investigate the role of serum alphafetoprotein variant ( AFP - L3 ) assay in the differential diagnosis of hepatocellular carcinoma ( HCC ) and cirrhostic diseases. Methods AFP - L3 was separated from sera of AFP -positive patients ( 45 HCC patients and 60 cirrhotic patients ) by micro - spin columns coupled with lens culinaris agglutinin ( LCA ). AFP was detected using electrochemiluminescence analysis to determine the amount of AFP - L3 and AFP - L3% ( the ratio of AFP - L3 to total AFP ). Results The AFP - L3% in HCC patients was significantly higher than that in patients with cirrhosis diseases ( F - 57. 433 , P = 0. 000 ). The area under curve of Receiver Operating Characteristic of AFP - 13 % was the highest ( 0. 916 ). The sensitivity and specificity of AFP - L3 for HCC patients was 92. 5 % and 84. 6 % respectively, with AFP -13% ≥15% being the optimal diagnostic threshold. Conclusion AFP - L3 ( % ) is more sensitive than the amounts of AFP and AFP - L3 in the diagnosis of HCC, and shows certain value in the differential diagnosis of HCC and cirrhosis diseases.%目的 探讨甲胎蛋白异质体3(AFP-L3)及其比值对原发性肝癌(HCC)和肝硬化的鉴别诊断价值及诊断界值.方法 应用AFP-L3快速分离微量离心柱分离40例HCC患者和65例肝硬化患者的AFP-L3,电化学发光免疫分析夹心法检测血清AFP水平,计算AFP-L3比值,即AFP-L3绝对值占血清AFP总量的百分比.结果 HCC患者AFP-L3比值大于肝硬化患者,差异有统计学意义(F=57.433,P=0.000);AFP -L3比值诊断HCC的受试者工作特征曲线(ROC曲线)下面积最大,为0.916;AFP-L3比值≥15%时诊断HCC的敏感度和特异度之和最大,为177.1%.结论 AFP-L3比值对HCC的诊断效能优于AFP和AFP-L3,对肝硬化和HCC的鉴别诊断具有重要临床意义.

  14. Clinical value of combined measurement of serum alpha-fetoprotein, alpha-L-fucosidase and ferritin levels in the diagnosis of primary liver cancer

    International Nuclear Information System (INIS)

    Objective: To investigate the clinical value of combined measurement of serum alpha-fetoprotein (AFP), alpha-L-fucosidase (AFU) and ferritin (SF) levels in the diagnosis of primary liver cancer. Methods: Serum AFP, AFU (with RIA) and SF (with biochemical method) were determined in 52 patients with primary liver cancer and 40 controls. Results: The positive rates of AFP, AFU and SF in patient with liver cancer were 82.7%, 86.6% and 76.9%, respectively. Positive rates with combined measurement of AFP plus AFU, AFP plus SF, and AFP plus AFU, SF were 94.2%, 90.4% and 98.1% respectively. Conclusion: Combined measurement of AFP, AFU and SF can significantly increase the positive rate in the diagnosis of primary liver cancer. (authors)

  15. Quantum-dot-based homogeneous time-resolved fluoroimmunoassay of alpha-fetoprotein

    Energy Technology Data Exchange (ETDEWEB)

    Chen Meijun; Wu Yingsong; Lin Guanfeng; Hou Jingyuan; Li Ming [Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, 510515 (China); Liu Tiancai, E-mail: liutc@smu.edu.cn [Institute of Antibody Engineering, School of Biotechnology, Southern Medical University, Guangzhou, 510515 (China)

    2012-09-05

    Highlights: Black-Right-Pointing-Pointer QDs-based homogeneous time-resolved fluoroimmunoassay was developed to detect AFP. Black-Right-Pointing-Pointer The conjugates were prepared with QDs-doped microspheres and anti-AFP McAb. Black-Right-Pointing-Pointer The conjugates were prepared with LTCs and another anti-AFP McAb. Black-Right-Pointing-Pointer Excess amounts of conjugates were used for detecting AFP without rinsing. Black-Right-Pointing-Pointer The wedding of QPs and LTCs was suitable for HTRFIA to detect AFP. - Abstract: Quantum dots (QDs) with novel photoproperties are not widely used in clinic diagnosis, and homogeneous time-resolved fluorescence assays possess many advantages over current methods for alpha-fetoprotein (AFP) detection. A novel QD-based homogeneous time-resolved fluorescence assay was developed and used for detection of AFP, a primary marker for many cancers and diseases. QD-doped carboxyl-modified polystyrene microparticles (QPs) were prepared by doping oil-soluble QDs possessing a 605 nm emission peak. The antibody conjugates (QPs-E014) were prepared from QPs and an anti-AFP monoclonal antibody, and luminescent terbium chelates (LTCs) were prepared and conjugated to a second anti-AFP monoclonal antibody (LTCs-E010). In a double-antibodies sandwich structure, QPs-E014 and LTCs-E010 were used for detection of AFP, serving as energy acceptor and donor, respectively, with an AFP bridge. The results demonstrated that the luminescence lifetime of these QPs was sufficiently long for use in a time-resolved fluoroassay, with the efficiency of time-resolved Foerster resonance transfer (TR-FRET) at 67.3% and the spatial distance of the donor to acceptor calculated to be 66.1 Angstrom-Sign . Signals from TR-FRET were found to be proportional to AFP concentrations. The resulting standard curve was log Y = 3.65786 + 0.43863{center_dot}log X (R = 0.996) with Y the QPs fluorescence intensity and X the AFP concentration; the calculated sensitivity was 0

  16. Prolonged Survival in a Case of Chemotherapy-Sensitive Gastric Cancer That Produced Alpha-Fetoprotein and Protein Induced by Vitamin K Antagonist-II

    OpenAIRE

    Ogasawara, Naotaka; Takahashi, Emiko; Matsumoto, Tomoko; Amaike, Manami; Nohara, Mako; Nagao, Kazuhiro; Ebi, Masahide; Funaki, Yasushi; Sasaki, Makoto; Kasugai, Kunio

    2015-01-01

    The number of reported cases of alpha-fetoprotein (AFP)-producing gastric cancer has gradually increased, with a reported prevalence of 1.3–1.5% of all gastric cancer cases. However, reports of gastric cancer accompanied by elevated serum levels of both AFP and protein induced by vitamin K antagonist-II (PIVKA-II) are rare. The prognosis of AFP- and PIVKA-II-producing gastric cancer has been reported to be very poor because the tumor cells were considered to have a high malignant potential an...

  17. Effect of serum alpha-fetoprotein and uric acid levels on pregnancy outcome in late pregnancy women with hypertensive disorder

    Institute of Scientific and Technical Information of China (English)

    Xian-Feng Tan; Yu-Xiu Zhang

    2016-01-01

    Objective:To investigate the effects of serum alpha-fetoprotein and uric acid levels on pregnancy outcome in late pregnancy women with hypertensive disorder.Methods: One hundred and twenty-six cases of hypertensive disorder complicating pregnancy were rolled into the observation group, and 80 healthy pregnant women were rolled into the as control group. The relationship between serum alpha-fetoprotein and uric acid levels and adverse pregnancy outcomes were compared between the two groups.Results: Compared with the control group, the serum levels of AFP and serum uric acid were significantly higher in the observation group, and the differences were statistically significant (P<0.01). In the observation group, with the increase of blood pressure, the serum AFP and uric acid levels were increased, which in the moderate sub-group and severe sub-group were significantly higher than those in mild sub-group, and the severe sub-group was significantly higher than that in the moderate sub-group (allP<0.05). The rate of preterm birth, premature rupture rate, placental abruption rate, fetal distress rate and cesarean section rate in the pregnant women with abnormal serum AFP and uric acid level were significantly higher than that of the normal serum AFP and uric acid group (P<0.05).Conclusions:The serum AFP and uric acid levels in the late pregnancy women with hypertensive disorder are significantly higher than those of the normal pregnant women, and are elevated remarkably as the blood pressure rising. When the serum AFP and uric acid levels are abnormal, the adverse outcomes of pregnancy and cesarean section rate increase significantly. Monitoring of serum AFP and uric acid levels is of importance.

  18. Contribution of alpha-fetoprotein in liver transplantation for hepatocellular carcinoma.

    Science.gov (United States)

    Charrière, Bérénice; Maulat, Charlotte; Suc, Bertrand; Muscari, Fabrice

    2016-07-28

    Alpha-fetoprotein (AFP) is the main tumor biomarker available for the management of hepatocellular carcinoma (HCC). Although it is neither a good screening test nor an accurate diagnostic tool for HCC, it seems to be a possible prognostic marker. However, its contribution in liver transplantation for HCC has not been fully determined, although its use to predict recurrence after liver transplantation has been underlined by international societies. In an era of organ shortages, it could also have a key role in the selection of patients eligible for liver transplantation. Yet unanswered questions remain. First, the cut-off value of serum AFP above which liver transplantation should not be performed is still a subject of debate. We show that a concentration of 1000 ng/mL could be an exclusion criterion, whereas values of AFP could also prove useful. However, evidence is lacking regarding the values that should be used. Today, the real input of AFP seems to be its integration into new criteria to select patients eligible for a liver transplantation. These recent tools have associated AFP values with morphological criteria, thus refining pre-existing criteria, such as Milan, University of California, San Francisco, or "up-to-seven". We provide a review of the different criteria submitted within the past years. Finally, AFP can be used to monitor recurrence after transplantation, although there is little evidence to support this claim. Future challenges will be to draft new international guidelines to implement the use of AFP as a selection tool, and to determine a clear cut-off value above which liver transplantation should not be performed. PMID:27478538

  19. Relationship between Expression of the Human Alpha-Fetoprotein Gene and DNA Methylation Status of the Promoter Region

    Institute of Scientific and Technical Information of China (English)

    Lijun Chen; Wei Wang; Qiuyue Jin; Ruimin Wang; Wenliang Hu

    2006-01-01

    OBJECTIVE DNA methylation has been regarded as an important epigenetic signature reflecting the transcription state of DNA in cells. This study was to conducted to assess the relationship between human alpha-fetoprotein (AFP) gene expression and the DNA methylation status of the promoter region in three different cells, namely two human hepatocellular carcinoma (HCC) cell lines and normal human fibroblasts.METHODS Transcription of the AFP gene was verified by RT-PCR. After bisulphate treatment of DNA, the methods of MSP and BSP were used to analyze the methylation density and status within single DNA strands of two closely spaced CpG dinucleotides of the promoter region in the different cells.RESULTS RT-PCR analysis indicated that the expression of the AFP gene in HepG2 cells was significantly higher than in SMMC-7721 cells,and that the AFP gene was not expressed in normal human fibroblasts.By MSP and BSP we observed that the promoter region was demethylated in the AFP-high-expressing cell lines, and that the sites of -2,494 bp and -2,431 bp in the AFP genomic sequence can be used as detection sites for early tumorous diagnosis.CONCLUSION These results indicate that the DNA methylation state of the promoter region has a negative correlation with AFP gene expression.

  20. Diagnostic Performance of Alpha-Fetoprotein, Protein Induced by Vitamin K Absence, Osteopontin, Dickkopf-1 and Its Combinations for Hepatocellular Carcinoma

    OpenAIRE

    Eun Sun Jang; Sook-Hyang Jeong; Jin-Wook Kim; Yun Suk Choi; Philippe Leissner; Christian Brechot

    2016-01-01

    Background & Aims Alpha-fetoprotein (AFP) is the most widely used serum biomarker for hepatocellular carcinoma (HCC), despite its limitations. As complementary biomarkers, protein induced by vitamin K absence (PIVKA-II), osteopontin (OPN), and Dickkopf-1 (DKK-1) have been proposed. This study aimed to perform a head-to-head comparison of the diagnostic performance of AFP, PIVKA-II, OPN and DKK-1 as single or in combination to seek the best biomarker or panel, and to investigate the clinical f...

  1. Maternal serum alpha fetoprotein in monozygotic and dizygotic twin pregnancies.

    Science.gov (United States)

    Thom, H; Buckland, C M; Campbell, A G; Thompson, B; Farr, V

    1984-01-01

    Maternal serum alpha-fetoprotein (MSAFP) values in the second trimester have been related to pregnancy outcome for 100 normal twin pairs, 42 monozygous (MZ) and 58 dizygous (DZ), liveborn after 28 weeks gestation. The median MSAFP value was 1.9 multiples of the median value (MOM) for uncomplicated singleton pregnancies. Both very low and very high MSAFP values were associated with twins of low birthweight. MSAFP values were higher in MZ than DZ twin pregnancies particularly those with dizygotes of like-sex. This effect was even more marked when only dichorionic like-sex twin pairs were compared. PMID:6209699

  2. Alpha-Fetoprotein and Novel Tumor Biomarkers as Predictors of Hepatocellular Carcinoma Recurrence after Surgery: A Brilliant Star Raises Again

    Directory of Open Access Journals (Sweden)

    Quirino Lai

    2012-01-01

    Full Text Available Alpha-fetoprotein (AFP, des-γ-carboxy prothrombin (DCP, and lens culinaris agglutinin-reactive fraction of AFP (AFP-L3 have been developed with the intent to detect hepatocellular carcinoma (HCC and for the surveillance of at-risk patients. However, at present, none of these tests can be recommended to survey cirrhotic patients at risk for HCC development because of their suboptimal ability for routine clinical practice in HCC diagnosis. Starting from these considerations, these markers have been therefore routinely and successfully used as predictors of survival and HCC recurrence in patients treated with curative intent. All these markers have been largely used as predictors in patients treated with hepatic resection or locoregional therapies, mainly in Eastern countries. In recent studies, AFP has been proposed as predictor of recurrence after liver transplantation and as selector of patients in the waiting list. Use of AFP modification during the waiting list for LT is still under investigation, potentially representing a very interesting tool for patient selection. The development of a new predictive model combining radiological and biological features based on biological markers is strongly required. New genetic markers are continuously discovered, but they are not already fully available in the clinical practice.

  3. Expression of alpha-fetoprotein messenger RNA in BEL-7404 human hepatoma cells and effect of L-4-oxalysine on the expression

    Institute of Scientific and Technical Information of China (English)

    1998-01-01

    AIM To investigate alpha-fetoprotein (AFP) mRNA expression in BEL-7404 human hepatoma cells and the effect of L-4-oxalysine (OXL) on the expression.METHODS BEl-7404 human hepatoma cells were maintained in RPMI 1640 media. Human AFP cDNA probe was labelled with digoxigenin-11-dUTP by the random primer labelling method. The expression of AFP mRNA in Bel-7404 cells was determined by an in situ hybridization technique with digoxigenin-labelled human AFP cDNA probe. The positive intensities of AFP mRNA in cells were analyzed by microspectrophotometer and expressed as absorbance at 470nm. For the experiment with OXL, cells were incubated with various concentrations of the agent for 72h.RESULTS Essentially all the hepatoma cells contained AFP mRNA in the cytoplasm, although in various amounts. The specificity of the hybridization reaction was confirmed by control experiments in which the use of RNase-treated BEL-7404 cells, non-AFP-producing cells (HL-60 human leukemia cells) or a nonspecific cDNA probe resulted in negative hybridization. When the cells were treated with OXL (25, 50mg/L), the content of AFP mRNA in the cytoplasm was decreased with the inhibition percentages of 34.3% and 70.1%, respectively (P<0.05).CONCLUSION AFP mRNA was expressed in BEL-7404 human hepatoma cells and OXL suppressed AFP mRNA expression in the cells.

  4. Telomerase inhibition decreases alpha-fetoprotein expression and secretion by hepatocellular carcinoma cell lines: in vitro and in vivo study.

    Directory of Open Access Journals (Sweden)

    Roula Tahtouh

    Full Text Available Alpha-fetoprotein (AFP is a diagnostic marker for hepatocellular carcinoma (HCC. A direct relationship between poor prognosis and the concentration of serum AFP has been observed. Telomerase, an enzyme that stabilizes the telomere length, is expressed by 90% of HCC. The aim of this study was to investigate the effect of telomerase inhibition on AFP secretion and the involvement of the PI3K/Akt/mTOR signaling pathway. Proliferation and viability tests were performed using tetrazolium salt. Apoptosis was determined through the Annexin V assay using flow cytometry. The concentrations of AFP were measured using ELISA kits. The AFP mRNA expression was evaluated using RT-PCR, and cell migration was evaluated using a Boyden chamber assay. The in vivo effect of costunolide on AFP production was tested in NSG mice. Telomerase inhibition by costunolide and BIBR 1532 at 5 and 10 μM decreased AFP mRNA expression and protein secretion by HepG2/C3A cells. The same pattern was obtained with cells treated with hTERT siRNA. This treatment exhibited no apoptotic effect. The AFP mRNA expression and protein secretion by PLC/PRF/5 was decreased after treatment with BIBR1532 at 10 μM. In contrast, no effect was obtained for PLC/PRF/5 cells treated with costunolide at 5 or 10 μM. Inhibition of the PI3K/Akt/mTOR signaling pathway decreased the AFP concentration. In contrast, the MAPK/ERK pathway appeared to not be involved in HepG2/C3A cells, whereas ERK inhibition decreased the AFP concentration in PLC/PRF/5 cells. Modulation of the AFP concentration was also obtained after the inhibition or activation of PKC. Costunolide (30 mg/kg significantly decreased the AFP serum concentration of NSG mice bearing HepG2/C3A cells. Both the inhibition of telomerase and the inhibition of the PI3K/Akt/mTOR signaling pathway decreased the AFP production of HepG2/C3A and PLC/PRF/5 cells, suggesting a relationship between telomerase and AFP expression through the PI3K

  5. Telomerase inhibition decreases alpha-fetoprotein expression and secretion by hepatocellular carcinoma cell lines: in vitro and in vivo study.

    Science.gov (United States)

    Tahtouh, Roula; Azzi, Anne-Sophie; Alaaeddine, Nada; Chamat, Soulaima; Bouharoun-Tayoun, Hasnaa; Wardi, Layal; Raad, Issam; Sarkis, Riad; Antoun, Najibe Abou; Hilal, George

    2015-01-01

    Alpha-fetoprotein (AFP) is a diagnostic marker for hepatocellular carcinoma (HCC). A direct relationship between poor prognosis and the concentration of serum AFP has been observed. Telomerase, an enzyme that stabilizes the telomere length, is expressed by 90% of HCC. The aim of this study was to investigate the effect of telomerase inhibition on AFP secretion and the involvement of the PI3K/Akt/mTOR signaling pathway. Proliferation and viability tests were performed using tetrazolium salt. Apoptosis was determined through the Annexin V assay using flow cytometry. The concentrations of AFP were measured using ELISA kits. The AFP mRNA expression was evaluated using RT-PCR, and cell migration was evaluated using a Boyden chamber assay. The in vivo effect of costunolide on AFP production was tested in NSG mice. Telomerase inhibition by costunolide and BIBR 1532 at 5 and 10 μM decreased AFP mRNA expression and protein secretion by HepG2/C3A cells. The same pattern was obtained with cells treated with hTERT siRNA. This treatment exhibited no apoptotic effect. The AFP mRNA expression and protein secretion by PLC/PRF/5 was decreased after treatment with BIBR1532 at 10 μM. In contrast, no effect was obtained for PLC/PRF/5 cells treated with costunolide at 5 or 10 μM. Inhibition of the PI3K/Akt/mTOR signaling pathway decreased the AFP concentration. In contrast, the MAPK/ERK pathway appeared to not be involved in HepG2/C3A cells, whereas ERK inhibition decreased the AFP concentration in PLC/PRF/5 cells. Modulation of the AFP concentration was also obtained after the inhibition or activation of PKC. Costunolide (30 mg/kg) significantly decreased the AFP serum concentration of NSG mice bearing HepG2/C3A cells. Both the inhibition of telomerase and the inhibition of the PI3K/Akt/mTOR signaling pathway decreased the AFP production of HepG2/C3A and PLC/PRF/5 cells, suggesting a relationship between telomerase and AFP expression through the PI3K/Akt/mTOR pathway. PMID:25822740

  6. Hepatitis B Virus X Protein Driven Alpha Fetoprotein Expression to Promote Malignant Behaviors of Normal Liver Cells and Hepatoma Cells

    Science.gov (United States)

    Zhu, Mingyue; Lu, Yan; Li, Wei; Guo, Junli; Dong, Xu; Lin, Bo; Chen, Yi; Xie, Xieju; Li, Mengsen

    2016-01-01

    Background: The infection of Hepatitis B virus (HBV) is closely associated with the development of hepatocellular carcinoma(HCC), HBV-X protein(HBx) is able to induce expression of alpha-fetoprotein(AFP) in normal liver cells, and AFP harbors a function to promote malignant transformation of normal liver cells, but the role AFP playing in malignant behaviors of HCC cells is still unclear. Methods: Fifty-six liver tissue samples were collected from the clinical patients through hepatectomy(include normal liver tissues, HBV-related hepatitis liver tissues and HBV-related HCC tissues), and diagnosis of these tissues by pathology section, expression of AFP, Ras and CXCR4 were evidenced by immunohisochemical staining and Western blotting; The proliferation of human normal liver cells line L-02 cells and human hepatoma cells line, HLE cells(non AFP-producing) were performed by MTT method; Repaired capacity of L-02 and HLE cells were compared by wound healing assay; Migration and invasion of these cells were analyzed by Transwell chamber assay; HBx expressed vectors(pcDNA3.1-HBx) were constructed and transfected into L-02 and HLE cells, effects of pcDNA3.1-HBx on the malignant behaviors were also detected by MTT, Transwell chamber assay and the expression of AFP, Ras and CXCR4 were evidenced by Western blotting. Results: we found that expression of AFP, Ras and CXCR4 in HBV-related HCC and lymph nodes metastasis tissues were significantly elevated compared with HBV-related HCC, non metastasis tissues and HBV-related hepatitis tissues; Expression of AFP, Ras and CXCR4 in HBV-related hepatitis tissues were significantly enhanced compared with normal liver tissues; The growth ratio, migratory and invasive ability, expression of AFP, Ras and CXCR4 of the cells were outstanding promoted while L-02 and HLE cells were transfected with pcDNA3.1-HBx vectors. The proliferation ratio, migration and invasion ability, and expression of Ras and CXCR4 were significantly inhibited while

  7. A highly sensitive biosensor for tumor maker alpha fetoprotein based on poly(ethylene glycol) doped conducting polymer PEDOT.

    Science.gov (United States)

    Cui, Min; Song, Zhiling; Wu, Yumin; Guo, Bing; Fan, Xiaojian; Luo, Xiliang

    2016-05-15

    Biocompatible polymers, such as poly(ethylene glycol) (PEG), are of great significance in the development of bio-interfaces and biosensors, as they possess excellent biocompatibility and are easy for modification. A novel highly biocompatible polymer composite was synthesized herein through electrochemical polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a PEG derivative, 4-arm PEG terminated with thiol groups. The electrodeposited conducting polymer composite of PEG doped PEDOT (PEDOT/PEG) exhibited flake-like nanostructure, large surface area and outstanding stability. In order to further immobilize antibodies, gold nanoparticles (AuNPs) were introduced to the PEDOT/PEG composite surface through their unique interaction with the thiol groups. AuNPs modified PEDOT/PEG provided a desirable support for the immobilization of various biomolecules such as antibodies for alpha fetoprotein (AFP), a vital tumor biomarker for liver cancer. The fabricated AFP biosensor demonstrated favorable selectivity, high sensitivity, and ultralow detection limit. Furthermore, owing to the presence of PEG polymers that are highly hydrophilic, such AuNPs/PEDOT/PEG based AFP biosensor also exhibited good anti-fouling ability, and it was capable of assaying target AFP in 10% (V/V) human serum samples, indicating highly feasible potential for clinical diagnosis. PMID:26774088

  8. Label-free alpha fetoprotein immunosensor established by the facile synthesis of a palladium-graphene nanocomposite.

    Science.gov (United States)

    Qi, TingTing; Liao, JinFeng; Li, YiSong; Peng, JinRong; Li, WenTing; Chu, BingYang; Li, He; Wei, YuQuan; Qian, ZhiYong

    2014-11-15

    In this study, we established a sensitive label-free immunosensor by palladium-reduced graphene oxide (Pd-rGO), which was prepared by one-pot synthesis under the reduction of extra-injected CO gas. The Pd-rGO nanocomposite structure has been confirmed by TEM, SEM, X-ray diffraction, and UV-vis spectroscopy. The Pd-rGO nanocomposite exhibited excellent stability in aqueous dispersion. The Pd-rGO-based label-free electrochemical immunosensor was used for detection of the hepatocellular carcinoma (HCC) biomarker alpha fetoprotein (AFP). The immunosensor determination was based on the fact that due to the formation of antigen-antibody immunocomplex, the decreased response amperometric currents of H2O2 were directly proportional to the concentrations of AFP. The limit of detection of this immunosensor for AFP detection is 5 pg/mL, and is linear from 0.01 to 12 ng/mL. The proposed immunosensor has been used to determine AFP in clinical serum samples with satisfactory results. This suggests the sensor may have great potential utility in the clinic. PMID:24906081

  9. A highly sensitive biosensor for tumor maker alpha fetoprotein based on poly(ethylene glycol) doped conducting polymer PEDOT.

    Science.gov (United States)

    Cui, Min; Song, Zhiling; Wu, Yumin; Guo, Bing; Fan, Xiaojian; Luo, Xiliang

    2016-05-15

    Biocompatible polymers, such as poly(ethylene glycol) (PEG), are of great significance in the development of bio-interfaces and biosensors, as they possess excellent biocompatibility and are easy for modification. A novel highly biocompatible polymer composite was synthesized herein through electrochemical polymerization of the conducting polymer poly(3,4-ethylenedioxythiophene) (PEDOT) and a PEG derivative, 4-arm PEG terminated with thiol groups. The electrodeposited conducting polymer composite of PEG doped PEDOT (PEDOT/PEG) exhibited flake-like nanostructure, large surface area and outstanding stability. In order to further immobilize antibodies, gold nanoparticles (AuNPs) were introduced to the PEDOT/PEG composite surface through their unique interaction with the thiol groups. AuNPs modified PEDOT/PEG provided a desirable support for the immobilization of various biomolecules such as antibodies for alpha fetoprotein (AFP), a vital tumor biomarker for liver cancer. The fabricated AFP biosensor demonstrated favorable selectivity, high sensitivity, and ultralow detection limit. Furthermore, owing to the presence of PEG polymers that are highly hydrophilic, such AuNPs/PEDOT/PEG based AFP biosensor also exhibited good anti-fouling ability, and it was capable of assaying target AFP in 10% (V/V) human serum samples, indicating highly feasible potential for clinical diagnosis.

  10. Significance of Combineddetecting Serum α-L-fucosidase (AFU), alpha-fetoprotein (AFP) andγ-glutamyl peptidase (GGT) for Earlydiagnosis ofhepatocellular Carcinoma (PHC)%AFP、AFU、GGT联合检测在原发性肝癌诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    安哲; 李伟; 屈梦

    2011-01-01

    目的 探讨血清α-L-岩藻糖苷酶(AFU)、甲胎蛋白(AFP)和γ-谷氨酰转肽酶(GGT)联合检测对提高原发性肝癌(PHC)阳性检出率的意义.方法 对50例原发性肝癌患者、30例肝硬化(LC)患者及30例健康人以AFP的检测为主体,配合GGT、AFU进行检测,优化组合,分析联检的结果.结果 AFP、AFU、GGT三项指标对PHC的阳性检出率分别为74%,82%,70%,AFP+AFU+GGT检测总阳性率达到98.85%,PHC组AFU、AFP与LC组和正常对照组均差异有显著性(P<0.05),LC组AFU、AFP、GGT与正常对照组比较差异有显著性(P<0.05).结论 应用AFU、AFP、GGT联合检测与AFP单项检测相比,PHC的诊断率明显提高.

  11. AFP-L3 — EDRN Public Portal

    Science.gov (United States)

    AFP-L3, also known as lectin-bound AFP, is an isoform of AFP, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. The L3 isoform is specific to malignant tumors and its detected presence may serve to identify patients who could benefit from monitoring for the development of HCC in high risk populations (i.e. chronic hepatitis, liver cirrhosis).

  12. Nanosilver-penetrated polyion graphene complex membrane for mediator-free amperometric immunoassay of alpha-fetoprotein using nanosilver-coated silica nanoparticles

    International Nuclear Information System (INIS)

    Research highlights: → We fabricate a polyion graphene complex membrane-based immunosensing platform for sensitive electrochemical immunoassay of alpha-fetoprotein. → Nanosilver-coated silica nanocomposites as bionanolabels. → Graphene nanosheets, single-stranded DNA and silver nanoparticles as matrices. → Direct electron transfer without electron mediator. → Analysis of real samples and method comparison. - Abstract: A facile and sensitive mediator-free electrochemical immunosensor for detection of alpha-fetoprotein (AFP) was designed by using nanosilver-coated silica nanoparticles (Ag-SiO2) as bionanolabels. To construct such an electrochemical immunosensor, silver ions/single-stranded DNA/graphene nanosheets were initially immobilized on a gold electrode in turn, then silver ions were in situ reduced to silver nanoparticles with the aid of NaBH4, and anti-AFP antibodies conjugated to silver nanoparticles were used. In the presence of AFP analyte, the sandwiched immunocomplex was formed on the electrode surface by using horseradish peroxidase-anti-AFP conjugate-labeled Ag-SiO2 (HRP-anti-AFP-Ag-SiO2) as secondary antibodies. Compared with pure silver nanoparticles, Ag-SiO2 nanocomposites could provide a large room for the immobilization of HRP-anti-AFP, and improve the electrochemical responses of the immunosensor. Meanwhile, the presence of highly conductive graphene nanosheets and silver nanoparticles provided a good pathway for electron transfer. Under optimal conditions, the immunosensor exhibited good electrochemical responses toward AFP ranging from 0.3 to 200 ng/mL with a detection limit (LOD) of 0.05 ng/mL (at 3σ) in pH 6.0 PBS-H2O2 system. Intra- and inter-assay displayed good precisions with coefficient of variation below 9.5%. In addition, the method was evaluated with 23 clinical serum samples, receiving good correlation with results from commercially available electrochemiluminescent analyzer.

  13. Nanosilver-penetrated polyion graphene complex membrane for mediator-free amperometric immunoassay of alpha-fetoprotein using nanosilver-coated silica nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Tang Juan [Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108 (China); Tang Dianping, E-mail: dianping.tang@fzu.edu.c [Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108 (China); Su Biling; Li Qunfang; Qiu Bin [Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108 (China); Chen Guonan, E-mail: gnchen@fzu.edu.c [Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108 (China)

    2011-04-15

    Research highlights: {yields} We fabricate a polyion graphene complex membrane-based immunosensing platform for sensitive electrochemical immunoassay of alpha-fetoprotein. {yields} Nanosilver-coated silica nanocomposites as bionanolabels. {yields} Graphene nanosheets, single-stranded DNA and silver nanoparticles as matrices. {yields} Direct electron transfer without electron mediator. {yields} Analysis of real samples and method comparison. - Abstract: A facile and sensitive mediator-free electrochemical immunosensor for detection of alpha-fetoprotein (AFP) was designed by using nanosilver-coated silica nanoparticles (Ag-SiO{sub 2}) as bionanolabels. To construct such an electrochemical immunosensor, silver ions/single-stranded DNA/graphene nanosheets were initially immobilized on a gold electrode in turn, then silver ions were in situ reduced to silver nanoparticles with the aid of NaBH{sub 4}, and anti-AFP antibodies conjugated to silver nanoparticles were used. In the presence of AFP analyte, the sandwiched immunocomplex was formed on the electrode surface by using horseradish peroxidase-anti-AFP conjugate-labeled Ag-SiO{sub 2} (HRP-anti-AFP-Ag-SiO{sub 2}) as secondary antibodies. Compared with pure silver nanoparticles, Ag-SiO{sub 2} nanocomposites could provide a large room for the immobilization of HRP-anti-AFP, and improve the electrochemical responses of the immunosensor. Meanwhile, the presence of highly conductive graphene nanosheets and silver nanoparticles provided a good pathway for electron transfer. Under optimal conditions, the immunosensor exhibited good electrochemical responses toward AFP ranging from 0.3 to 200 ng/mL with a detection limit (LOD) of 0.05 ng/mL (at 3{sigma}) in pH 6.0 PBS-H{sub 2}O{sub 2} system. Intra- and inter-assay displayed good precisions with coefficient of variation below 9.5%. In addition, the method was evaluated with 23 clinical serum samples, receiving good correlation with results from commercially available

  14. In vitro radionuclide therapy and in vivo scintigraphic imaging of alpha fetoprotein producing hepatocellular carcinoma by targeted sodium iodide symporter gene expression

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Kwang Il; Lee, Yong Jin; Lee, Tae Sup; Song, Inho; Cheon, Gi Jeong; Lim, Sang Moo; Kang, Joo Hyun [Korea Institute of Radiological and Medical and Medical Sciences, Seoul (Korea, Republic of); Chung, June Key [Seoul National Univ. College of Medicine, Seoul (Korea, Republic of)

    2012-03-15

    This study aimed to develop a gene expression targeting method for specific imaging and therapy of alpha fetoprotein (AFP) producing hepatocellular carcinoma (HCC) cells, using an adenovirus vector containing the human sodium/iodide symporter (hNIS) gene driven by an AFP enhancer/promoter. The recombinant adenovirus vector, AdAFPhNIS (containing the hNIS gene driven by human AFP enhancer/promoter) was prepared. After in vitro infection by the adenovirus, hNIS gene expression in AFP producing cells and in AFP nonproducing cells was investigated using {sup 125}I uptake assay and semi quantitative reverse transcription polymerase chain reaction (RT-PCR). The killing effect of {sup 131}I vitro clonogenic assay. In addition, tumor bearing mice were intravenously injected with the adenovirus, and scintigraphic images were obtained. The expression of hNIS was efficiently demonstrated by {sup 125}I uptake assay in AFP producing cells, but not in AFP nonproducing cells. AFP producing HCC targeted gene expression was confirmed at the mRNA level. Furthermore, in vitro clonogenic assay showed that hNIS gene expression induced by AdAFPhNIS infection in AFP producing cells caused more sensitivity to {sup 131}I than that in AFP nonproducing cells. Injected intravenously in HuH-7 tumor xenografts mice by adenovirus, the functional hNIS gene expression was confirmed in tumor by in vivo scintigraphic imaging. An AFP producing HCC was targeted with an adenovirus vector containing the hNIS gene using the AFP enhancer/promoter in vitro and in vivo. These findings demonstrate that AFP producing HCC specific molecular imaging and radionuclide gene therapy are feasible using this recombinant adenovirus vector system.

  15. Female mice deficient in alpha-fetoprotein show female-typical neural responses to conspecific-derived pheromones.

    Directory of Open Access Journals (Sweden)

    Olivier Brock

    Full Text Available The neural mechanisms controlling sexual behavior are sexually differentiated by the perinatal actions of sex steroid hormones. We recently observed using female mice deficient in alpha-fetoprotein (AFP-KO and which lack the protective actions of AFP against maternal estradiol, that exposure to prenatal estradiol completely defeminized the potential to show lordosis behavior in adulthood. Furthermore, AFP-KO females failed to show any male-directed mate preferences following treatment with estradiol and progesterone, indicating a reduced sexual motivation to seek out the male. In the present study, we asked whether neural responses to male- and female-derived odors are also affected in AFP-KO female mice. Therefore, we compared patterns of Fos, the protein product of the immediate early gene, c-fos, commonly used as a marker of neuronal activation, between wild-type (WT and AFP-KO female mice following exposure to male or estrous female urine. We also tested WT males to confirm the previously observed sex differences in neural responses to male urinary odors. Interestingly, AFP-KO females showed normal, female-like Fos responses, i.e. exposure to urinary odors from male but not estrous female mice induced equivalent levels of Fos protein in the accessory olfactory pathways (e.g. the medial part of the preoptic nucleus, the bed nucleus of the stria terminalis, the amygdala, and the lateral part of the ventromedial hypothalamic nucleus as well as in the main olfactory pathways (e.g. the piriform cortex and the anterior cortical amygdaloid nucleus, as WT females. By contrast, WT males did not show any significant induction of Fos protein in these brain areas upon exposure to either male or estrous female urinary odors. These results thus suggest that prenatal estradiol is not involved in the sexual differentiation of neural Fos responses to male-derived odors.

  16. RIA of alpha-fetoprotein in serum and amniotic fluid

    International Nuclear Information System (INIS)

    An own modification of the double antibody radioimmunoassay for AFP using 125I-labelled AFP as a tracer, rabbit anti-AFP obtained from SEVAC, Prague and precipitating antibodies prepared by the authors is described. The AFP levels measured in the serum and the amniotic fluid using the method were in agreement with those obtained by the means of the AFPK RIA kit by SORIN in the Institute for the Care of Mother and Child in Prague. The AFP concentrations found in the cord serum and the amniotic fluid were confirmed also by the rocket electroimmunoassay according to Laurell. The described AFP RIA seems suitable for the clinical application in prenatal screening for congenital malformations, in difficult pregnancies, in hepatology and the diagnosis and the evaluation of therapy of some human malignancies. (author)

  17. Des-gamma carboxy prothrombin (PIVKA-II) and alpha-fetoprotein producing gastric cancer with multiple liver metastases.

    Science.gov (United States)

    Takahashi, Yoshihisa; Inoue, Tohru

    2003-04-01

    We describe the case of an 87-year-old woman who presented to Tokyo Kousei Nenkin Hospital because of appetite loss and general fatigue. Multiple liver masses and Borrmann type 2 gastric tumor were detected. A clinical diagnosis of hepatocellular carcinoma and gastric cancer was made based on the patient's high levels of serum alpha-fetoprotein (AFP; 490 200 ng/mL) and protein induced by vitamin K absence or antagonist-II (PIVKA-II, 2284 mAU/mL). The patient's general condition worsened gradually and she died 42 days after admission. Autopsy revealed that the predominant histological structure of the gastric tumor was trabecular or sheet-like, although a tubular structure was also found. Venous invasion was prominent. Immunohistochemically, the tumor tissue was positive for AFP and a few tumor cells were positive for PIVKA-II. The histological appearance and immunohistochemical features of the hepatic tumors resembled that of the gastric tumor. This case was pathologically diagnosed as AFP- and PIVKA-II-producing gastric carcinoma with multiple liver metastases. When tumors are found in the stomach and liver and serum PIVKA-II level is abnormally high, the possibility of PIVKA-II-producing gastric cancer with liver metastasis should be considered, especially when hepatitis virus markers are negative and liver cirrhosis is not present.

  18. Propensity Score Matching Analysis of Changes in Alpha-Fetoprotein Levels after Combined Radiotherapy and Transarterial Chemoembolization for Hepatocellular Carcinoma with Portal Vein Tumor Thrombus.

    Directory of Open Access Journals (Sweden)

    Yuri Jeong

    Full Text Available To investigate the value of changes in alpha-fetoprotein (AFP levels for the prediction of radiologic response and survival outcomes in hepatocellular carcinoma (HCC patients with portal vein tumor thrombus (PVTT who received combined treatment of 3-dimensional conformal radiotherapy (3D-CRT and transarterial chemoembolization (TACE.A database of 154 HCC patients with PVTT and elevated AFP levels (>20 ng/mL treated with 3D-CRT and TACE as an initial treatment between August 2002 and August 2008 was retrospectively reviewed. AFP levels were determined 1 month after radiotherapy, and AFP response was defined as an AFP level reduction of >20% from the initial level. Radiologic response, overall survival (OS, and progression-free survival (PFS rates were compared between AFP responders and non-responders. Propensity-score based matching analysis was performed to minimize the effect of potential confounding bias.The median follow-up period was 11.1 months (range, 3.1-82.7 months. In the propensity-score matching cohort (92 pairs, a best radiologic response of CR or PR occurred in more AFP responders than AFP non-responders (41.3% vs. 10.9%, p < 0.001. OS and PFS were also longer in AFP responders than in non-responders (median OS 13.2 months vs. 5.6 months, p < 0.001; median PFS 8.7 months vs. 3.5 months, p < 0.001.AFP response is a significant predictive factor for radiologic response. Furthermore, AFP response is significant for OS and PFS outcomes. AFP evaluation after combined radiotherapy and TACE appears to be a useful predictor of clinical outcomes in HCC patients with PVTT.

  19. Abnormal maternal serum alpha fetoprotein and pregnancy outcome.

    Science.gov (United States)

    Zarzour, S J; Gabert, H A; Diket, A L; St Amant, M; Miller, J M

    1998-01-01

    The objective was to assess the occurrence of miscarriages, low birth weight, and karyotype abnormalities found with low and elevated maternal serum alpha-fetoprotein (MSAFP) among women who had genetic amniocentesis performed. A retrospective study of 2,159 women who had MSAFP analysis prior to amniocentesis was conducted. Pregnancy outcomes were obtained from record review and physicians follow-up. Limits of MSAFP used in analysis were MOM) (lower levels) and >2.0 MOM (upper levels). Autosomal trisomy was found in 1.6% with low, 0.9% normal, and 0.6% with elevated MSAFP values. Sex chromosome abnormalities were present only in patients with normal MSAFP, [45X (n = 6), 47XXY (n = 2), 69XXX]. Of five open neural tube defects, four had elevated MSAFP and one had a normal value. Omphalocele was identified in four patients, two with normal and two with elevated MSAFP. Gastroschisis was found in one low and one elevated MSAFP. Amniotic fluid alpha-fetoprotein (AFAFP) values did not correlate with MSAFP values. Patients with low MSAFP levels had a greater prevalence of abnormal karyotype (19 of 249, prevalence = 0.076) than patients with an elevated MSAFP level (2 or 166, prevalence = 0.012 OR (odds ratio) = 0.20 (P value = 0.024) when unadjusted for maternal age, and OR = 0.09 (P value = 0.001) when adjusted for maternal age. Spontaneous abortion occurred more often in patients with elevated (4 of 166, or 4%) than normal or low (20 of 1948, or 1%) values of MSAFP (odds ratio 4.32, P = 0.020 when adjusted for maternal age). Birth weight below 2,500 g was present less frequently with low or normal MSAFP (136 of 1,760, or 7.7%) than in elevated MSAFP (21 of 144 or 14.6%) (odds ratio 2.04, P = 0.005, unadjusted; and odds ratio = 2.32, P = 0.003, adjusted for maternal age). Female fetuses were present more often with low MSAFP (136 of 249, or 55%) than elevated levels 43% (71 of 164, or 43%; P = 0.024). We conclude that patients undergoing genetic amniocentesis with MSAFP

  20. Amperometric immunosensor for {alpha}-fetoprotein antigen in human serum based on co-immobilizing dinuclear copper complex and gold nanoparticle doped chitosan film

    Energy Technology Data Exchange (ETDEWEB)

    Gan Ning; Meng Linghua; Wang Feng [State Key Laboratory Base of Novel Functional Materials and Preparation science, Faculty of Material Science and Chemical Engineering of Ninbo University, Ningbo, 315211 (China)], E-mail: ganning@nbu.edu.cn

    2009-09-01

    A sensitive amperometric immunosensor for {alpha}-fetoprotein (AFP), a tumor marker for the diagnosis of hepatocellular carcinoma (HCC), was constructed, The immunosensor is prepared by co-immobilizing [Cu{sub 2}(phen){sub 2}Cl{sub 2}] ({mu}-Cl){sub 2} (CuL), nano-Au/Chitosan(Chit) composite, horseradish peroxidase (HRP) and AFP antibody(anti-AFP) on a glassy carbon electrode (GCE). Firstly, CuL was irreversibly absorb on GCE electrode through {pi}-{pi} stacking interaction; then nano-Au/Chit composite was immobilized onto the electrode because of its excellent membrane-forming ability, finally HRP and anti-AFP was adsorbed onto the surface of the gold nanoparticles to construct GCE | CuL/nanoAu-chit/HRP/anti-AFP immunosensor. The preparation procedure of the electrode was characterized by electrochemical and spectroscopy method. The results showed that this immunosensor exhibited an excellent electrocatalytic response to the reduction of hydrogen peroxide (H{sub 2}O{sub 2}) without the aid of an electron mediator, offers a high-sensitivity (1710 nA {center_dot} ng{sup -1} {center_dot} ml{sup -1}) for the detection of AFP and has good correlation for detection of AFP in the range of 0.2 to 120.0 ng/ml with a detection limit of 0.05 ng/ml. The biosensor showed high selectivity as well as good stability and reproductivity.

  1. Diagnostic value of alpha-fetoprotein in liver cancer

    International Nuclear Information System (INIS)

    Objective: To determine diagnostic value of alpha-fetoproteins (alpha-FP) in liver cancer. Design: Prospective study. Place and duration of study: Department of clinical oncology services Hospital Lahore, during the period from February 1998 to February 2001. Subjects and Methods: Among 200 persons studied, 100 presented with liver mass, jaundice and other symptoms directing toward liver pathology, later confirmed histopathologically, as suffering from hepatocellular carcinoma (HCC) while the other 100 healthy subject came to the department for blood donation and were HBs Ag pasitive on blood screening. All these subjects under went blood test for alpha-FP. This tumor marker was analyzed by using enzyme immunoassay-based kit. Results: The alpha-FP positivity was statistically evaluated. In HCC this test was statistically significant with p value of <0.001. In this study sensitivity of alpha-FP was 72% specificity 89%, positive predictive value 86.7% and negative predictive value of 76.1%. Conclusion: This study showed that alpha-FP was a useful diagnostic tool in the diagnosis of HCC. (author)

  2. Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles

    Directory of Open Access Journals (Sweden)

    Huang KW

    2012-06-01

    Full Text Available Kai-Wen Huang,1 Jen-Jie Chieh,2 Herng-Er Horng,2 Chin-Yih Hong,3 Hong-Chang Yang41Department of Surgery and Hepatitis Research Center, National Taiwan University Hospital, Taipei, 2Institute of Electro-optical Science and Technology, National Taiwan Normal University, Taipei, 3Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, 4Department of Physics, National Taiwan University, Taipei, TaiwanAbstract: For preoperative and intraoperative detection of tumor distribution, numerous multimodal contrast agents, such as magnetic nanoparticles (MNPs with several examination indicators, are currently in development. However, complex materials, configuration, and cost are required for multimodal contrast agents, accompanied by a high possibility of toxicity and low popularity in clinics. Nevertheless, the magnetic labeling of MNPs using bioprobes should be feasible not only in preoperative magnetic resonance imaging (MRI, but also in intraoperative examination based on other magnetic properties. In this study, anti-alpha-fetoprotein (AFP-mediated Fe3O4 MNPs, injected into mice with liver tumors, were used to examine the characteristics of magnetic labeling. Using MRI and scanning superconducting-quantum-interference-device biosusceptometry (SSB, based on alternating current (AC susceptibility, the magnetic labeling occurred significantly on the first day post-injection of anti-AFP magnetic fluid (MF, and then decreased over time. However, for both MF without antibodies and an anti-carcinoembryonic antigen MF, no magnetic labeling occured on the first day of their respective post-injection. The favorable agreement indicates that magnetic labeling possesses two magnetic characteristics: distortion of the imaging field and AC susceptibility. In addition, the results of the biopsy tests, anti-AFP staining, and Prussian blue staining show the same dynamics as those of magnetic methodologies and prove that bound MNPs on

  3. Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips.

    Science.gov (United States)

    Liang, Rong-Liang; Xu, Xu-Ping; Liu, Tian-Cai; Zhou, Jian-Wei; Wang, Xian-Guo; Ren, Zhi-Qi; Hao, Fen; Wu, Ying-Song

    2015-09-01

    Alpha-fetoprotein (AFP), a primary marker for many diseases including various cancers, is important in clinical tumor diagnosis and antenatal screening. Most immunoassays provide high sensitivity and accuracy for determining AFP, but they are expensive, often complex, time-consuming procedures. A simple and rapid point-of-care system that integrates Eu (III) chelate microparticles with lateral flow immunoassay (LFIA) has been developed to determine AFP in serum with an assay time of 15 min. The approach is based on a sandwich immunoassay performed on lateral flow test strips. A fluorescence strip reader was used to measure the fluorescence peak heights of the test line (HT) and the control line (HC); the HT/HC ratio was used for quantitation. The Eu (III) chelate microparticles-based LFIA assay exhibited a wide linear range (1.0-1000 IU mL(-1)) for AFP with a low limit of detection (0.1 IU mL(-1)) based on 5ul of serum. Satisfactory specificity and accuracy were demonstrated and the intra- and inter-assay coefficients of variation (CV) for AFP were both chelate microparticles-based LFIA system provided a rapid, sensitive and reliable method for determining AFP in serum, indicating that it would be suitable for development in point-of-care testing.

  4. Clinical Utility of Alpha Fetoprotein and HCCR-1, Alone or in Combination, in Patients with Chronic Hepatitis, Liver Cirrhosis and Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Peng Jirun

    2011-01-01

    Full Text Available Serum alpha fetoprotein (AFP is the most widely used tumor marker in detecting patients with hepatocellular carcinoma (HCC. However, it has been indicated that HCCR-1 (human cervical cancer oncogene 1 might be supplementary to AFP in the detection. We conducted a prospective study in 120 normal and 524 liver disease patients to evaluate the significance of simultaneous measurement of 2 tumor markers (AFP and HCCR-1 in the diagnosis of HCC through the cohort study in Korea and China. We also performed immunohistochemical studies using 25 normal subjects (N, 32 liver cirrhosis (LC and 116 HCC tissues. The sensitivities of AFP (20 ng/mL and HCCR-1 (10 ng/mL in HCC were 55.8% (164/294 and 44.2% (130/294, respectively. When AFP was combined with HCCR-1, sensitivities increased to 4.2% (N, 12.7% (chronic hepatitis; CH, 50.0% (LC, and 77.2% (HCC, respectively. Although there was no significant difference in the diagnostic rate for HCC between AFP and HCCR-1, many cases for AFP-negative HCC were positive for HCCR-1 and vice versa. Moreover, the combined use of AFP and HCCR-1 improved the diagnostic rate to 70.8% in small HCC (< 2 cm and 81.6% in large HCC (≥ 2 cm, respectively. AFP and HCCR-1 are independent markers. Our result suggests that the HCCR-1 could be an useful biomarker for HCC while the diagnostic rate could be significantly improved in the combined use of HCCR-1 and AFP.

  5. First-trimester maternal serum alpha-fetoprotein as a marker for fetal chromosomal disorders. Dutch Working Party on Prenatal Diagnosis.

    Science.gov (United States)

    Van Lith, J M

    1994-10-01

    We evaluated first-trimester maternal serum alpha-fetoprotein (MS-AFP) as a marker for fetal chromosomal disorders. The multicentre study was performed under the auspices of the Dutch Working Party on Prenatal Diagnosis. MS-AFP was measured in 2404 normal pregnancies and 72 chromosomally abnormal pregnancies. The median multiple of the normal median (MOM) in 32 Down's syndrome pregnancies was 0.83 with a 95 per cent confidence interval ranging from 0.60 to 1.04. The difference between the distributions of first-trimester MS-AFP in normal and Down's syndrome pregnancies was statistically significant (t-test: t = 2.34, P MOM = 1.26; seven cases with sex chromosome abnormalities, MOM = 1.07; 22 cases with a chromosomal mosaic pattern in chorionic villi, MOM = 1.08). We conclude that first-trimester MS-AFP can discriminate between normal and Down's syndrome pregnancies, but is not an effective marker. First-trimester MS-AFP has no value as a marker for other fetal chromosomal disorders. PMID:7534926

  6. Effect of increasing maximal aerobic exercise on serum gonadal hormones and alpha-fetoprotein in the luteal phase of professional female soccer players.

    Science.gov (United States)

    Otağ, Aynur; Hazar, Muhsin; Otağ, İlhan; Beyleroğlu, Malik

    2016-03-01

    [Purpose] The performance of female athletes during their menstrual period has attracted the attention of researchers for many years. It is known that the menstrual period changes with exercise. Alpha-fetoprotein (AFP) is an oncofetal protein. In this study, the effect of maximal aerobic exercise in the luteal phase on some hormones and AFP in female athletes was researched. [Subjects and Methods] Twelve volunteers and healthy female footballers with normal menstrual cycles volunteered for this study as subjects. All the participants performed a shuttle run test. Blood samples were taken before, after, and one hour after exercise. Serum AFP, estrogen, progesterone, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) values were measured using an auto analyzer and original kits. Heart rate measurements were performed before and after the exercise. [Results] AFP activity had significantly decreased after 1 h of recovery from the exercise in the female soccer players, and estrogen and LH activity had significantly increased immediately after the exercise. Progesterone activity had significantly decreased immediately after the exercise. FSH values had significantly increased immediately after the exercise. [Conclusion] The results of the present study show there were significant decreases in the values of AFP, which is a cancer parameter, 1 hour after the exercise. This result may be valuable in future physiotherapy studies on the relationship between exercise and cancer. PMID:27134362

  7. Prolonged Survival in a Case of Chemotherapy-Sensitive Gastric Cancer That Produced Alpha-Fetoprotein and Protein Induced by Vitamin K Antagonist-II

    Directory of Open Access Journals (Sweden)

    Naotaka Ogasawara

    2015-04-01

    Full Text Available The number of reported cases of alpha-fetoprotein (AFP-producing gastric cancer has gradually increased, with a reported prevalence of 1.3-1.5% of all gastric cancer cases. However, reports of gastric cancer accompanied by elevated serum levels of both AFP and protein induced by vitamin K antagonist-II (PIVKA-II are rare. The prognosis of AFP- and PIVKA-II-producing gastric cancer has been reported to be very poor because the tumor cells were considered to have a high malignant potential and the cancer progressed rapidly. We described a case of gastric cancer producing AFP and PIVKA-II in which chemotherapy was effective and resulted in prolonged survival, and these two tumor markers were useful for monitoring the treatment response. Routine health screening using upper abdominal ultrasonography revealed hepatic tumors in an apparently healthy 65-year-old man. Whole-body computed tomography (CT revealed multiple hepatic tumors, and an esophagogastroduodenoscopy (EGD revealed a Bormann type 3 tumor in the lower stomach. A biopsy specimen confirmed that the tumor was immunohistochemically positive for AFP, PIVKA-II, and human epidermal growth factor receptor 2. After chemotherapy, the gastric tumor appeared as a small elevated lesion on EGD, and CT revealed a remarkable reduction in the size of the metastatic liver tumors. The patient is still alive, 35 months after the initial chemotherapy.

  8. Prolonged Survival in a Case of Chemotherapy-Sensitive Gastric Cancer That Produced Alpha-Fetoprotein and Protein Induced by Vitamin K Antagonist-II.

    Science.gov (United States)

    Ogasawara, Naotaka; Takahashi, Emiko; Matsumoto, Tomoko; Amaike, Manami; Nohara, Mako; Nagao, Kazuhiro; Ebi, Masahide; Funaki, Yasushi; Sasaki, Makoto; Kasugai, Kunio

    2015-01-01

    The number of reported cases of alpha-fetoprotein (AFP)-producing gastric cancer has gradually increased, with a reported prevalence of 1.3-1.5% of all gastric cancer cases. However, reports of gastric cancer accompanied by elevated serum levels of both AFP and protein induced by vitamin K antagonist-II (PIVKA-II) are rare. The prognosis of AFP- and PIVKA-II-producing gastric cancer has been reported to be very poor because the tumor cells were considered to have a high malignant potential and the cancer progressed rapidly. We described a case of gastric cancer producing AFP and PIVKA-II in which chemotherapy was effective and resulted in prolonged survival, and these two tumor markers were useful for monitoring the treatment response. Routine health screening using upper abdominal ultrasonography revealed hepatic tumors in an apparently healthy 65-year-old man. Whole-body computed tomography (CT) revealed multiple hepatic tumors, and an esophagogastroduodenoscopy (EGD) revealed a Bormann type 3 tumor in the lower stomach. A biopsy specimen confirmed that the tumor was immunohistochemically positive for AFP, PIVKA-II, and human epidermal growth factor receptor 2. After chemotherapy, the gastric tumor appeared as a small elevated lesion on EGD, and CT revealed a remarkable reduction in the size of the metastatic liver tumors. The patient is still alive, 35 months after the initial chemotherapy. PMID:26034473

  9. Characteristics of magnetic labeling on liver tumors with anti-alpha-fetoprotein-mediated Fe3O4 magnetic nanoparticles.

    Science.gov (United States)

    Huang, Kai-Wen; Chieh, Jen-Jie; Horng, Herng-Er; Hong, Chin-Yih; Yang, Hong-Chang

    2012-01-01

    For preoperative and intraoperative detection of tumor distribution, numerous multimodal contrast agents, such as magnetic nanoparticles (MNPs) with several examination indicators, are currently in development. However, complex materials, configuration, and cost are required for multimodal contrast agents, accompanied by a high possibility of toxicity and low popularity in clinics. Nevertheless, the magnetic labeling of MNPs using bioprobes should be feasible not only in preoperative magnetic resonance imaging (MRI), but also in intraoperative examination based on other magnetic properties. In this study, anti-alpha-fetoprotein (AFP)-mediated Fe(3)O(4) MNPs, injected into mice with liver tumors, were used to examine the characteristics of magnetic labeling. Using MRI and scanning superconducting-quantum-interference-device biosusceptometry (SSB), based on alternating current (AC) susceptibility, the magnetic labeling occurred significantly on the first day post-injection of anti-AFP magnetic fluid (MF), and then decreased over time. However, for both MF without antibodies and an anti-carcinoembryonic antigen MF, no magnetic labeling occured on the first day of their respective post-injection. The favorable agreement indicates that magnetic labeling possesses two magnetic characteristics: distortion of the imaging field and AC susceptibility. In addition, the results of the biopsy tests, anti-AFP staining, and Prussian blue staining show the same dynamics as those of magnetic methodologies and prove that bound MNPs on tumor tissue are rotatable by an AC magnetic field to express AC susceptibility. Therefore, with the simple configuration of antibody-mediated MNPs, magnetic labeling is also feasible for intraoperative examinations using SSB with high mobility and sensitivity. PMID:22787394

  10. Diagnostic Performance of Alpha-Fetoprotein, Protein Induced by Vitamin K Absence, Osteopontin, Dickkopf-1 and Its Combinations for Hepatocellular Carcinoma.

    Directory of Open Access Journals (Sweden)

    Eun Sun Jang

    Full Text Available Alpha-fetoprotein (AFP is the most widely used serum biomarker for hepatocellular carcinoma (HCC, despite its limitations. As complementary biomarkers, protein induced by vitamin K absence (PIVKA-II, osteopontin (OPN, and Dickkopf-1 (DKK-1 have been proposed. This study aimed to perform a head-to-head comparison of the diagnostic performance of AFP, PIVKA-II, OPN and DKK-1 as single or in combination to seek the best biomarker or panel, and to investigate the clinical factors affecting their performance.Using 401 stored plasma samples obtained from 208 HCC patients and 193 liver cirrhosis control patients, plasma AFP, PIVKA-II, OPN and DKK-1 levels were measured by ELISA, and receiver operating characteristic curve analyses were performed for each biomarker and for every combination of two to four markers.Of the four biomarkers, AFP showed the highest area under the curve (0.786. The sensitivity and specificity for each single biomarker was 62% and 90.2% (AFP>20 ng/mL, 51.0% and 91.2% (PIVKA-II>10 ng/mL, 46.2% and 80.3% (OPN>100 ng/mL, and 50.0% and 80.8% (DKK-1>500 pg/mL, respectively. Among the combinations of two biomarkers, AFP>20 ng/mL or DKK-1>500 pg/mL showed the best diagnostic performance (sensitivity 78.4%, specificity 72.5%. Triple or quadruple combination did not improve the diagnostic performance further. The patient's age, etiology and tumor invasiveness of HCC affected the performance of each marker.AFP was the most useful single biomarker for HCC diagnosis, and the combined measurement of AFP and DKK-1 could maximize the diagnostic yield. Clinical decision should be based on the consideration of various factors affecting the diagnostic performance of each biomarker. Efforts to seek novel HCC biomarkers should be continued.

  11. A fluorescent molecularly-imprinted polymer gate with temperature and pH as inputs for detection of alpha-fetoprotein.

    Science.gov (United States)

    Karfa, Paramita; Roy, Ekta; Patra, Santanu; Kumar, Deepak; Madhuri, Rashmi; Sharma, Prashant K

    2016-04-15

    In this work, we have reported a new approach on the use of stimuli-responsive molecularly imprinted polymer (MIP) for trace level sensing of alpha-fetoprotein (AFP), which is a well know cancer biomarker. The stimuli-responsive MIP is composed of three components, a thermo-responsive monomer, a pH responsive component (tyrosine derivative) and a highly fluorescent vinyl silane modified carbon dot. The synthesized AFP-imprinted polymer possesses excellent selectivity towards their template molecule and dual-stimuli responsive behavior. Along with this, the imprinted polymer was also explored as 'OR' logic gate with two stimuli (pH and temperature) as inputs. However, the non-imprinted polymers did not have such 'OR' gate property, which confirms the role of template binding. The imprinted polymer was also used for estimation of AFP in the concentration range of 3.96-80.0 ng mL(-1), with limit of detection (LOD) 0.42 ng mL(-1). The role of proposed sensor was successfully exploited for analysis of AFP in real human blood plasma, serum and urine sample.

  12. In vivo screening of hepatocellular carcinoma using AC susceptibility of anti-alpha fetoprotein-activated magnetic nanoparticles.

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    Jen-Jie Chieh

    Full Text Available With antibody-mediated magnetic nanoparticles (MNPs applied in cancer examinations, patients must pay at least twice for MNP reagents in immunomagnetic reduction (IMR of in vitro screening and magnetic resonance imaging (MRI of in vivo tests. This is because the high maintenance costs and complex analysis of MRI have limited the possibility of in vivo screening. Therefore, this study proposes novel methods for in vivo screening of tumors by examining the AC susceptibility of bound MNPs using scanning superconducting-quantum-interference-device (SQUID biosusceptometry (SSB, thereby demonstrating high portability and improved economy. The favorable agreement between in vivo tests using SSB and MRI demonstrated the feasibility of in vivo screening using SSB for hepatocellular carcinoma (HCC targeted by anti-alpha fetoprotein (AFP-mediated MNPs. The magnetic labeling was also proved by in vitro tests using SSB and biopsy assays. Therefore, patients receiving bioprobe-mediated MNPs only once can undergo in vivo screening using SSB in the future.

  13. Higher Ratio of Serum Alpha-Fetoprotein Could Predict Outcomes in Patients with Hepatitis B Virus-Associated Hepatocellular Carcinoma and Normal Alanine Aminotransferase.

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    Young-Il Kim

    Full Text Available The role of serum alpha-fetoprotein (AFP levels in the surveillance and diagnosis of hepatocellular carcinoma (HCC is controversial. The aim of this study was to investigate the value of serially measured serum AFP levels in HCC progression or recurrence after initial treatment.A total of 722 consecutive patients newly diagnosed with HCC and treated at the National Cancer Center, Korea, between January 2004 and December 2009 were enrolled. The AFP ratios between 4-8 weeks post-treatment and those at the time of HCC progression or recurrence were obtained. Multivariate logistic regression analysis was performed to correlate the post-treatment AFP ratios with the presence of HCC progression or recurrence.The etiology of HCC was related to chronic hepatitis B virus (HBV infection in 562 patients (77.8%, chronic hepatitis C virus (HCV infection in 74 (10.2%, and non-viral cause in 86 (11.9%. There was a significant decrease in serum AFP levels from the baseline to 4 to 8 weeks after treatment (median AFP, 319.6 ng/mL vs. 49.6 ng/mL; p 1.0 was an independently associated with HCC progression or recurrence. Among the different causes of HCC analyzed, this association was significant only for HCC related to chronic hepatitis B (p< 0.001 and non-viral causes (p<0.05, and limited only to patients who had normal alanine aminotransferase (ALT levels.Serial measurements of serum AFP ratios could be helpful in detecting progression or recurrence in treated patients with HBV-HCC and normal ALT.

  14. Expression of AFP and Rev-Erb A/Rev-Erb B and N-CoR in fetal rat liver, liver injury and liver regeneration

    OpenAIRE

    Meier, Volker; Tron, Kyrylo; Batusic, Danko; Elmaouhoub, Abderrahim; Ramadori, Giuliano

    2006-01-01

    Background Alpha-fetoprotein (AFP) expression can resume in the adult liver under pathophysiological conditions. Orphan nuclear receptors were supposed to regulate AFP gene expression, in vitro. We were interested to study the expression of AFP and orphan nuclear receptors, in vivo. Results The expression of AFP gene and orphan nuclear receptors in the liver was examined in different rat models: (a) fetal liver (b) liver regeneration [partial hepatectomy (PH) with and without 2-acetyl-aminofl...

  15. Magnetic immunoassay coupled with inductively coupled plasma mass spectrometry for simultaneous quantification of alpha-fetoprotein and carcinoembryonic antigen in human serum

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Xing; Chen, Beibei; He, Man; Zhang, Yiwen; Xiao, Guangyang; Hu, Bin, E-mail: binhu@whu.edu.cn

    2015-04-01

    The absolute quantification of glycoproteins in complex biological samples is a challenge and of great significance. Herein, 4-mercaptophenylboronic acid functionalized magnetic beads were prepared to selectively capture glycoproteins, while antibody conjugated gold and silver nanoparticles were synthesized as element tags to label two different glycoproteins. Based on that, a new approach of magnetic immunoassay-inductively coupled plasma mass spectrometry (ICP-MS) was established for simultaneous quantitative analysis of glycoproteins. Taking biomarkers of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as two model glycoproteins, experimental parameters involved in the immunoassay procedure were carefully optimized and analytical performance of the proposed method was evaluated. The limits of detection (LODs) for AFP and CEA were 0.086 μg L{sup −1} and 0.054 μg L{sup −1} with the relative standard deviations (RSDs, n = 7, c = 5 μg L{sup −1}) of 6.5% and 6.2% for AFP and CEA, respectively. Linear range for both AFP and CEA was 0.2–50 μg L{sup −1}. To validate the applicability of the proposed method, human serum samples were analyzed, and the obtained results were in good agreement with that obtained by the clinical chemiluminescence immunoassay. The developed method exhibited good selectivity and sensitivity for the simultaneous determination of AFP and CEA, and extended the applicability of metal nanoparticle tags based on ICP-MS methodology in multiple glycoprotein quantifications. - Highlights: • 4-Mercaptophenylboronic acid functionalized magnetic beads were prepared and characterized. • ICP-MS based magnetic immunoassay approach was developed for quantification of glycoproteins. • AFP and CEA were quantified simultaneously with Au and Ag NPs as element tags. • The developed method exhibited good selectivity and sensitivity for target glycoproteins.

  16. Magnetic immunoassay coupled with inductively coupled plasma mass spectrometry for simultaneous quantification of alpha-fetoprotein and carcinoembryonic antigen in human serum

    International Nuclear Information System (INIS)

    The absolute quantification of glycoproteins in complex biological samples is a challenge and of great significance. Herein, 4-mercaptophenylboronic acid functionalized magnetic beads were prepared to selectively capture glycoproteins, while antibody conjugated gold and silver nanoparticles were synthesized as element tags to label two different glycoproteins. Based on that, a new approach of magnetic immunoassay-inductively coupled plasma mass spectrometry (ICP-MS) was established for simultaneous quantitative analysis of glycoproteins. Taking biomarkers of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) as two model glycoproteins, experimental parameters involved in the immunoassay procedure were carefully optimized and analytical performance of the proposed method was evaluated. The limits of detection (LODs) for AFP and CEA were 0.086 μg L−1 and 0.054 μg L−1 with the relative standard deviations (RSDs, n = 7, c = 5 μg L−1) of 6.5% and 6.2% for AFP and CEA, respectively. Linear range for both AFP and CEA was 0.2–50 μg L−1. To validate the applicability of the proposed method, human serum samples were analyzed, and the obtained results were in good agreement with that obtained by the clinical chemiluminescence immunoassay. The developed method exhibited good selectivity and sensitivity for the simultaneous determination of AFP and CEA, and extended the applicability of metal nanoparticle tags based on ICP-MS methodology in multiple glycoprotein quantifications. - Highlights: • 4-Mercaptophenylboronic acid functionalized magnetic beads were prepared and characterized. • ICP-MS based magnetic immunoassay approach was developed for quantification of glycoproteins. • AFP and CEA were quantified simultaneously with Au and Ag NPs as element tags. • The developed method exhibited good selectivity and sensitivity for target glycoproteins

  17. Diagnostic sensitivity of serum carcinoembryonic antigen, carbohydrate antigen 19-9, alpha-fetoprotein, and beta-human chorionic gonadotropin in esophageal carcinoma (receiver operating characteristic curve analysis

    Directory of Open Access Journals (Sweden)

    Bhawna Bagaria

    2015-01-01

    Full Text Available Background: Esophageal carcinomas are very lethal disease relatively unresponsive to therapy. The continued development of new and more effective chemotherapeutic agents and regimens offers hope that in the future, this carcinoma may be amenable to either more effective palliative treatment or possibly increased cure. We, therefore, aimed to evaluate the marker with best diagnostic sensitivity in esophageal carcinoma. Materials and Methods: Serum carcinoembryonic antigen (CEA, carbohydrate antigen 19-9 (CA19-9, alpha-fetoprotein (AFP, and beta-human chorionic gonadotropin (β-HCG levels were assessed in healthy subjects (n = 50 and patients (n = 50 initially diagnosed of esophageal carcinoma by endoscopic examination and biopsy before receiving any therapy. The data were analyzed using SPSS software version 10.0 (SPSS Inc. USA and MedCalc to estimate mean ± standard deviation, the significance of the observed differences (P value, for calculating sensitivity and for plotting receiver operating characteristic curves. Results: Sensitivity of CEA, CA19-9, AFP, and β-HCG detected in esophagus cancer was 38%, 18%, 10%, and 26% respectively. Conclusion: From the above studied markers, CEA has the highest sensitivity followed by β-HCG, CA19-9 and AFP. Although the sensitivity of tumor markers in esophagus cancer is low, they may be useful additional parameter in the prediction of neoplasms involved at the early stage of tumor growth.

  18. Facile fabrication of a silicon nanowire sensor by two size reduction steps for detection of alpha-fetoprotein biomarker of liver cancer

    Science.gov (United States)

    Binh Pham, Van; ThanhTung Pham, Xuan; Nhat Khoa Phan, Thanh; Thanh Tuyen Le, Thi; Chien Dang, Mau

    2015-12-01

    We present a facile technique that only uses conventional micro-techniques and two size-reduction steps to fabricate wafer-scale silicon nanowire (SiNW) with widths of 200 nm. Initially, conventional lithography was used to pattern SiNW with 2 μm width. Then the nanowire width was decreased to 200 nm by two size-reduction steps with isotropic wet etching. The fabricated SiNW was further investigated when used with nanowire field-effect sensors. The electrical characteristics of the fabricated SiNW devices were characterized and pH sensitivity was investigated. Then a simple and effective surface modification process was carried out to modify SiNW for subsequent binding of a desired receptor. The complete SiNW-based biosensor was then used to detect alpha-fetoprotein (AFP), one of the medically approved biomarkers for liver cancer diagnosis. Electrical measurements showed that the developed SiNW biosensor could detect AFP with concentrations of about 100 ng mL-1. This concentration is lower than the necessary AFP concentration for liver cancer diagnosis.

  19. Sandwich immunoassay for alpha-fetoprotein in human sera using gold nanoparticle and magnetic bead labels along with resonance Rayleigh scattering readout

    International Nuclear Information System (INIS)

    We describe a sensitive sandwich immunoassay for alpha-fetoprotein (AFP). It is making use of gold nanoparticles (GNPs) and magnetic beads (MBs) as labels, and of resonance Rayleigh scattering for detection. Two antibodies were labeled with GNPs and MBs, respectively, and MB-antigen-GNP complexes were formed in the presence of antigens. The MB labels also serve as solid phase carriers that can be used to magnetically separate the immuno complex. The GNP labels are used as optical probes, and Rayleigh scattering was used to determine the concentration of free GNPs-antibody after separation of the MB-antigen-GNP complexes. The concentration of AFP is related to the intensity of light scattered by free GNPs in the 13.6 pM to 436 pM concentration range, and the limit of detection is 13.6 pM. The method was applied to the determination of AFP in sera of cancer patients, and the results agree well with those obtained by conventional ELISA. (author)

  20. Highly sensitive electrochemical immunosensor for the detection of alpha fetoprotein based on PdNi nanoparticles and N-doped graphene nanoribbons.

    Science.gov (United States)

    Li, Na; Ma, Hongmin; Cao, Wei; Wu, Dan; Yan, Tao; Du, Bin; Wei, Qin

    2015-12-15

    An ultrasensitive sandwich-type electrochemical immunosensor was designed for the quantitative detection of alpha fetoprotein (AFP). The β-cyclodextrins functionalized graphene sheets (CD-GS) were used as the sensing matrix for immobilizing adamantine-1-carboxylic acid functionalized primary anti-AFP (ADA-Ab1) and enhanced the electron transfer. PdNi alloy nanoparticles decorated N-doped graphene nanoribbons (PdNi/N-GNRs) were used as labels of secondary anti-AFP (Ab2), and PdNi alloy nanoparticles (PdNi NPs) exhibited high catalytic performance towards the reduction of H2O2. Meanwhile, with good dispersion, large specific surface area and good catalytic performance, N-doped graphene nanoribbons (N-GNRs) significantly amplified the electrochemical signal. Under the optimal conditions, the electrochemical immunosensor exhibited a wide linear range of 0.0001-16 ng/mL with a low detection limit of 0.03 pg/mL. Additionally, the proposed immunosensor showed high specificity, good reproducibility and long-term stability, which have promising application in bioassay analysis.

  1. Fifty years of discovery of alpha-fetoprotein as the first tumor marker

    Directory of Open Access Journals (Sweden)

    Nikulina Dina

    2015-01-01

    Full Text Available Alpha-fetoprotein represents the most prominent oncobiomarker, widely used in the diagnosis of hepatocellular carcinoma for monitoring of tumor progression, presence of metastasis, assessment of cancer prognosis and successful antitumor therapeutic measures. Yuri Semenovich Tatarinov is a Russian scientist who first published antigen specific for human hepatocellular carcinoma in 1963. To commemorate the 50th anniversary of the discovery of alpha-fetoprotein, 9th International Scientific- Practical Conference entitled “Achievements of fundamental science and translational medicine capabilities in solving actual problems of practical public health”, was held from May 6-8th, 2013 in Astrakhan, Russia. The conference was held in memory of historical scientific work of Yuri Semenovich Tatarinov. [Projekat Ministarstva nauke Republike Srbije, br. 175056

  2. Utility-based criteria for selecting patients with hepatocellular carcinoma for liver transplantation: A multicenter cohort study using the alpha-fetoprotein model as a survival predictor.

    Science.gov (United States)

    Vitale, Alessandro; Farinati, Fabio; Burra, Patrizia; Trevisani, Franco; Giannini, Edoardo G; Ciccarese, Francesca; Piscaglia, Fabio; Rapaccini, Gian Lodovico; Di Marco, Mariella; Caturelli, Eugenio; Zoli, Marco; Borzio, Franco; Cabibbo, Giuseppe; Felder, Martina; Sacco, Rodolfo; Morisco, Filomena; Missale, Gabriele; Foschi, Francesco Giuseppe; Gasbarrini, Antonio; Svegliati Baroni, Gianluca; Virdone, Roberto; Chiaramonte, Maria; Spolverato, Gaya; Cillo, Umberto

    2015-10-01

    The lifetime utility of liver transplantation (LT) in patients with hepatocellular carcinoma (HCC) is still controversial. The aim of this study was to ascertain when LT is cost-effective for HCC patients, with a view to proposing new transplant selection criteria. The study involved a real cohort of potentially transplantable Italian HCC patients (n = 2419 selected from the Italian Liver Cancer group database) who received nontransplant therapies. A non-LT survival analysis was conducted, the direct costs of therapies were calculated, and a Markov model was used to compute the cost utility of LT over non-LT therapies in Italian and US cost scenarios. Post-LT survival was calculated using the alpha-fetoprotein (AFP) model on the basis of AFP values and radiological size and number of nodules. The primary endpoint was the net health benefit (NHB), defined as LT survival benefit in quality-adjusted life years minus incremental costs (US $)/willingness to pay. The calculated median cost of non-LT therapies per patient was US $53,042 in Italy and US $62,827 in the United States. On Monte Carlo simulation, the NHB of LT was always positive for AFP model values ≤ 3 and always negative for values > 7 in both countries. A multivariate model showed that nontumor variables (patient's age, Child-Turcotte-Pugh [CTP] class, and alternative therapies) had the potential to shift the AFP model threshold of LT cost-ineffectiveness from 3 to 7. LT proved always cost-effective for HCC patients with AFP model values ≤ 3, whereas the cost-ineffectiveness threshold ranged between 3 and 7 using nontumor variables.

  3. A novel electrochemical immunosensor using β-cyclodextrins functionalized silver supported adamantine-modified glucose oxidase as labels for ultrasensitive detection of alpha-fetoprotein.

    Science.gov (United States)

    Gao, Jian; Ma, Hongmin; Lv, Xiaohui; Yan, Tao; Li, Na; Cao, Wei; Wei, Qin

    2015-09-17

    In this work, a novel sandwich-type electrochemical immunosensor based on host-guest interaction was fabricated for the detection of alpha-fetoprotein (AFP). Due to the large specific surface area of multiwalled carbon nanotubes and the unique supramolecular recognition ability of β-cyclodextrins, ferrocenecarboxylic acid (Fc) was incorporated into this sensor platform by host-guest interaction to generate an electrochemical signal. And β-cyclodextrins functionalized silver supported adamantine-modified glucose oxidase (GOD-CD-Ag), was used as a label to improve the analytical performance of the immunosensor by the dual amplification strategy. The obtained GOD-CD-Ag conjugates could convert glucose into gluconic acid with the formation of hydrogen peroxide (H2O2). And then silver nanoparticles could in situ catalyze the reduction of the generated H2O2, dramatically improving the oxidation reaction of Fc. The developed immunosensor shows a wide linear calibration range from 0.001 to 5.0 ng/mL with a low detection limit (0.2 pg/mL) for the detection of AFP. The method, with ideal reproducibility and selectivity, has a wide application prospect in clinical research.

  4. Mechanism of Cancer Growth Suppression of Alpha-Fetoprotein Derived Growth Inhibitory Peptides (GIP): Comparison of GIP-34 versus GIP-8 (AFPep). Updates and Prospects

    Energy Technology Data Exchange (ETDEWEB)

    Mizejewski, Gerald J. [Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201 (United States)

    2011-06-20

    The Alpha-fetoprotein (AFP) derived Growth Inhibitory Peptide (GIP) is a 34-amino acid segment of the full-length human AFP molecule that inhibits tumor growth and metastasis. The GIP-34 and its carboxy-terminal 8-mer segment, termed GIP-8, were found to be effective as anti-cancer therapeutic peptides against nine different human cancer types. Following the uptake of GIP-34 and GIP-8 into the cell cytoplasm, each follows slightly different signal transduction cascades en route to inhibitory pathways of tumor cell growth and proliferation. The parallel mechanisms of action of GIP-34 versus GIP-8 are demonstrated to involve interference of signaling transduction cascades that ultimately result in: (1) cell cycle S-phase/G2-phase arrest; (2) prevention of cyclin inhibitor degradation; (3) protection of p53 from inactivation by phosphorylation; and (4) blockage of K{sup +} ion channels opened by estradiol and epidermal growth factor (EGF). The overall mechanisms of action of both peptides are discussed in light of their differing modes of cell attachment and uptake fortified by RNA microarray analysis and electrophysiologic measurements of cell membrane conductance and resistance. As a chemotherapeutic adjunct, the GIPs could potentially aid in alleviating the negative side effects of: (1) tamoxifen resistance, uterine hyperplasia/cancer, and blood clotting; (2) Herceptin antibody resistance and cardiac (arrest) arrhythmias; and (3) doxorubicin's bystander cell toxicity.

  5. Mechanism of Cancer Growth Suppression of Alpha-Fetoprotein Derived Growth Inhibitory Peptides (GIP): Comparison of GIP-34 versus GIP-8 (AFPep). Updates and Prospects

    International Nuclear Information System (INIS)

    The Alpha-fetoprotein (AFP) derived Growth Inhibitory Peptide (GIP) is a 34-amino acid segment of the full-length human AFP molecule that inhibits tumor growth and metastasis. The GIP-34 and its carboxy-terminal 8-mer segment, termed GIP-8, were found to be effective as anti-cancer therapeutic peptides against nine different human cancer types. Following the uptake of GIP-34 and GIP-8 into the cell cytoplasm, each follows slightly different signal transduction cascades en route to inhibitory pathways of tumor cell growth and proliferation. The parallel mechanisms of action of GIP-34 versus GIP-8 are demonstrated to involve interference of signaling transduction cascades that ultimately result in: (1) cell cycle S-phase/G2-phase arrest; (2) prevention of cyclin inhibitor degradation; (3) protection of p53 from inactivation by phosphorylation; and (4) blockage of K+ ion channels opened by estradiol and epidermal growth factor (EGF). The overall mechanisms of action of both peptides are discussed in light of their differing modes of cell attachment and uptake fortified by RNA microarray analysis and electrophysiologic measurements of cell membrane conductance and resistance. As a chemotherapeutic adjunct, the GIPs could potentially aid in alleviating the negative side effects of: (1) tamoxifen resistance, uterine hyperplasia/cancer, and blood clotting; (2) Herceptin antibody resistance and cardiac (arrest) arrhythmias; and (3) doxorubicin's bystander cell toxicity

  6. Alpha-fetoprotein, identified as a novel marker for the antioxidant effect of placental extract, exhibits synergistic antioxidant activity in the presence of estradiol.

    Directory of Open Access Journals (Sweden)

    Hye Yeon Choi

    Full Text Available Placenta, as a reservoir of nutrients, has been widely used in medical and cosmetic materials. Here, we focused on the antioxidant properties of placental extract and attempted to isolate and identify the main antioxidant factors. Porcine placental extracts were prepared through homogenization or acid hydrolysis, and their antioxidant activity was investigated in the human keratinocyte HaCaT cell line. Treatment with homogenized placental extract (H-PE increased the cell viability of H2O2-treated HaCaT cells more than two-fold. H-PE treatment suppressed H2O2-induced apoptotic and necrotic cell death and decreased intracellular ROS levels in H2O2-treated HaCaT cells. The antioxidant factors in H-PE were found to be thermo-unstable and were thus expected to include proteins. The candidate antioxidant proteins were fractionated with cation-exchange, anion-exchange, and size-exclusion chromatography, and the antioxidant properties of the chromatographic fractions were investigated. We obtained specific antioxidant fractions that suppressed ROS generation and ROS-induced DNA strand breaks. From silver staining and MALDI-TOF analyses, alpha-fetoprotein (AFP precursor was identified as a main marker for the antioxidant effect of H-PE. Purified AFP or ectopically expressed AFP exhibited synergistic antioxidant activity in the presence of estradiol. Taken together, our data suggest that AFP, a serum glycoprotein produced at high levels during fetal development, is a novel marker protein for the antioxidant effect of the placenta that exhibits synergistic antioxidant activity in the presence of estradiol.

  7. Optimization of AFP-radioimmunoassay using Antibody Capture Technique

    International Nuclear Information System (INIS)

    Alpha-fetoprotein (AFP) is a substance produced by the unborn baby. When the neural tube is not properly formed large amounts of AFP pass into the amniotic fluid and reach the mother's blood. By measuring AFP in the mother's blood and amniotic fluid, it is possible to tell whether or not there is a chance that the unborn baby has a neural tube defect. AFP also used as a tumor marker for hepatocellular carcinoma. There are many different techniques for measuring AFP in blood, but the most accurate one is the immunoassay technique. The immunoassays can be classified on the basis of methodology into three classes; (1) the antibody capture assays, (2) the antigen capture assay, (3)the two-antibody sandwich assays. In this present study, the antibody capture assay in which the antigen is attached to a solid support, and labeled antibody is allowed to bind, will be optimized

  8. The progressive elevation of alpha fetoprotein for the diagnosis of hepatocellular carcinoma in patients with liver cirrhosis

    Directory of Open Access Journals (Sweden)

    Flores-Estrada Diana

    2007-02-01

    Full Text Available Abstract Background Hepatocellular carcinoma is the most common cause of primary liver neoplasms and is one of the main causes of death in patients with liver cirrhosis. High Alpha fetoprotein serum levels have been found in 60–70% of patients with Hepatocellular carcinoma; nevertheless, there are other causes that increase this protein. Alpha fetoprotein levels ≥200 and 400 ng/mL in patients with an identifiable liver mass by imaging techniques are diagnostic of hepatocellular carcinoma with high specificity. Methods We analysed the sensitivity and specificity of the progressive increase of the levels of alpha fetoprotein for the detection of hepatocellular carcinoma in patients with liver cirrhosis. Seventy-four patients with cirrhosis without hepatocellular carcinoma and 193 with hepatic lesions diagnosed by biopsy and shown by image scans were included. Sensitivity and specificity of transversal determination of alpha fetoprotein ≥ 200 and 400 ng/mL and monthly progressive elevation of alpha fetoprotein were analysed. Areas under the ROC curves were compared. Positive and negative predictive values adjusted to a 5 and 10% prevalence were calculated. Results For an elevation of alpha fetoprotein ≥ 200 and 400 ng/mL the specificity is of 100% in both cases, with a sensitivity of 36.3 and 20.2%, respectively. For an alpha fetoprotein elevation rate ≥7 ng/mL/month, sensitivity was of 71.4% and specificity of 100%. The area under the ROC curve of the progressive elevation was significantly greater than that of the transversal determination of alpha fetoprotein. The positive and negative predictive values modified to a 10% prevalence are of: 98.8% and 96.92%, respectively; while for a prevalence of 5% they were of 97.4% and 98.52%, respectively. Conclusion The progressive elevation of alpha fetoprotein ≥7 ng/mL/month in patients with liver cirrhosis is useful for the diagnosis of hepatocellular carcinoma in patients that do not reach

  9. Dosage of alpha-fetoprotein for radioimmunoassay in maternal blood. Its obstetrical application

    Energy Technology Data Exchange (ETDEWEB)

    Pandolfo, J.; Jaumandreu, C.A. (Clinica Ginecotocologica ' ' A' ' (Uruguay)); Aguirre de Garcia, B.; Robles, A.M.; Touya, E. (Centro de Medicina Nuclear y Centro de Nefrologia (Uruguay))

    1981-08-01

    Leigthon, Gordon and col. showed that the determination of serum levels of alpha-fetoprotein was useful as a method for the detection of fetal malformations of the neural tube. Due to its important clinical significance, the study of this simple test in women in different stages of pregnancy has been started. A total of 34 patients from the obstetrical external service were studied, with pregnancy stages between 10 and 40 weeks. Having established the incidence and implications of false positive and principally of false negative results, it has been concluded that this technique should be imposed as routine in the evolutive control of pregnancy.

  10. Clinical application of alpha-fetoprotein variant to screening benign and mlaignant liver dis-eases%甲胎蛋白异质体在鉴别良恶性肝病中的临床应用

    Institute of Scientific and Technical Information of China (English)

    陶绍能; 周建国; 程光华; 戴云海; 张新路; 阮昊

    2014-01-01

    目的:探讨小扁豆凝集素结合型甲胎蛋白异质体( AFP-L3)在良恶性肝病鉴别诊断中的临床价值。方法:应用装有耦联了小扁豆凝集素( LCA)的微量离心柱分离131例肝病患者的AFP-L3,用化学发光免疫检测血清AFP和AFP-L3含量,计算AFP-L3占AFP总量的百分比( AFP-L3%)。结果:与肝硬化( LC)和慢性肝炎( CH)组相比,肝癌( HCC)组中患者血清AFP、AFP-L3与AFP-L3%均升高,经Kruskal-Wallis 秩和检验有统计学意义(χ2=31.457,41.983,413.77;P=0.000,0.000,0.000)。AFP、AFP-L3和AFP-L3%相应ROC曲线下面积(AUC)分别为0.628、0.718和0.795,其差异均有统计学意义(P<0.05)。以AFP-L3%≥6.90%判断为阳性,AFP-L3%诊断肝细胞癌敏感性和特异性分别为66.7%和82.4%。结论:AFP-L3对肝细胞癌诊断准确度明显高于AFP,微量离心柱法检测AFP-L3在良恶性肝脏病变鉴别诊断中具有重要临床价值。%Objective:To verify the clinical value of applying the isoform of alpha -fetoprotein variant(AFP-L3) to screening benign and malignant liver diseases.Methods:AFP-L3 was isolated 131 patients with liver disease using the micro-spin column coupled with lens culinaris agglutinin ( LCA) .Chemi-luminescent immunoassay was performed to determine the content of serum AFP and AFP-L3,and AFP-L3% was calculated.Results:The serum levels of AFP and AFP-L3 as well as AFP-L3% were significantly higher in patients with hepatocellular carcinoma (HCC) compared to patients with liver cirrhosis (LC) and chronic hepatitis (CH),and Kruskal-Wallis one-way analysis of the variance showed statistical difference (χ2 =31.457,41.983,41.377;P=0.000,respectively).The area under the ROC curve (AUC) of AFP,AFP-L3 and AFP-L3 % was 0.628,0.718 and 0.795,respectively,and the differ-ences were significant (P<0.05).Sensitivity and specificity by AFP-L3 %(≥6.90% cut-off value) for diagnosis of HCC were66 .7

  11. The Comparative Study on Ultrastructure and Immunohistochemistry in AFP Negative and Positive Hepatocellular Carcinoma

    Institute of Scientific and Technical Information of China (English)

    郑美蓉; 阮幼冰; 杨木兰; 官阳; 武忠弼

    2004-01-01

    To comparatively investigate ultrastructural characteristics and expressions of AFP(alpha-fetoprotein) and Tn (Thomsen-Friedenreich-related antigen) protein in AFP negative (AFP-)and AFP positive (AFP+) primary hepatocellular carcinoma. Fourty-three cases of AFP- and AFP+ hepatocellular carcinoma (HCC) tissues and five cases of normal liver tissues were divided into three groups: control group (normal liver tissue, n=5); AFP+ HCC group (the serum AFP level was higher than 10 ng/ml, n = 22); AFP- HCC group (the serum AFP level was lower than 10 ng/ml, n=21). The ultrastructural morphology was studied by transmission electron microscopy, the expressions of AFP and Tn protein were detected by immunohistochemistry and cell image analysis. 1. The immunohistochemical study showed that (1) the expression intensity and positive rate of Tn protein in AFP- HCC group were markedly higher than that in AFP+ HCC group (P<0.01); (2) The expression intensity of AFP in AFP- HCC group was lower than that in AFP+ HCC group (P<0.01 ). 2. The transmission electron microscopy demonstrated that some AFP- HCC cells linked closely with each other, others dispersed loosely just as cultured cells, the remarkable morphologic features in AFP- HCC cells were simple organelles, But they were abundant in the free polyribosomes. In AFP+ HCC group, all the HCC cells linked closely together and were rich organelles in their cytoplasm, especially the rough endoplasmic reticula. In addition, mitochondria and Golgi complex were obviously observed. (1) The AFP and Tn protein had discrepancy distribution in AFP- and AFP+ HCC tissues, Tn protein may be one of the early diagnostic indicators in AFP- HCC; (2) The synthetic locations of the AFP and Tn protein were different in hepatocarcinoma cells by ultrastructural observation.

  12. Immunoregulatory effects of AFP domains on monocyte-derived dendritic cell function

    Directory of Open Access Journals (Sweden)

    Wibowo Gunawan A

    2011-01-01

    Full Text Available Abstract Background Alpha-fetoprotein (AFP is a tumor-associated glycoprotein that functions in regulation of both ontogenic and oncogenic growth. Recent study showed that AFP can induce apoptosis or impair monocyte-derived dendritic cell (MDDC function. However, it is still unclear which AFP domain (D-AFP plays major role in this function. Results As expected monocytes cultured in the presence of Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF and Interleukin-4 (IL-4 developed into MDDC. Up-regulation of HLA-DR and CD11c as well as loss of CD14 molecules could be observed. Full length AFP (FL-AFP, domain 2 AFP (D2-AFP and D3-AFP, but not D1-AFP, significantly inhibited the expression of HLA-DRhigh/CD11chigh and CD80+/CD86high molecules. In contrast, CD83 expression was substantially down-regulated in all samples. Expression of CD40 was significantly suppressed by FL-AFP but not by any D-AFPs. Finally, both FL-AFP and D-AFP impaired the MDDC ability to secrete IL-12 (p70. Conclusions D2- and D3- but not D1-AFP extensively suppresses the MDDC function. All the recombinant AFP proteins impaired the ability of MDDC to secrete IL-12.

  13. [The clinical significance of PIVKA-II determination in patients with hepatocellular carcinoma: a comparative study with alpha-fetoprotein].

    Science.gov (United States)

    Sakon, M; Monden, M; Goto, M; Kanai, T; Umeshita, K; Endo, W; Mori, T

    1990-05-01

    The changes in the plasma level of PIVKA-II (Protein Induced by Vitamin K Absence or Antagonist-II) following the treatment or progress of the disease was studied in 60 patients with hepatocellular carcinoma. The positivity rate determined by the changes in PIVKA-II was 58.4 percent (35/60 cases) and was about the same as those reported so far, all of which were obtained by a single determination of PIVKA-II. Plasma PIVKA-II was elevated in 61.9 percent (13/21 cases) of alpha-fetoprotein negative patients and it was almost identical with the overall positivity rate. In parallel with serum alpha-fetoprotein, the plasma level of PIVKA-II was decreased after the surgery or transcatheter arterial embolization and was increased when the recurrence or progress of the disease was observed. Furthermore, the nonspecific elevation of PIVKA-II due to the associated liver cirrhosis or chronic hepatitis was infrequent compared with that of alpha-fetoprotein. In 18 cases positive with both PIVKA-II and alpha-fetoprotein, a close correlation (R = 0.91) was observed between the changes of these markers during the progress or treatment of the disease. Thus, it was suggested that determination of PIVKA-II in blood might be useful not only in the diagnosis but in monitoring the progress or the effectiveness of treatments in hepatocellular carcinoma.

  14. Heat shock protein 70 chaperoned alpha-fetoprotein in human hepatocellular carcinoma cell line BEL-7402

    Institute of Scientific and Technical Information of China (English)

    Xiao-Ping Wang; Qiao-Xia Wang; Hai-Yan Li; Rui-Fen Chen

    2005-01-01

    AIM: To investigate the interaction between heat shock protein 70 (HSP70) and α-fetoprotein (AFP) in human hepatocellular carcinoma (HCC) cell line BEL7402.METHODS: The expression and localization of HSP70 and AFP in human HCC cell line BEL-7402 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. The interaction between HSP70 and AFP in HCC cells was analyzed by immunoprecipitation and Western blot.RESULTS: Immunocytochemical staining detection showed that HCC cell BEL-7402 expressed a high level of HSP70 and AFP synchronously. Both were stained in cell plasma.AFP existed in the immunoprecipitate of anti-HSP70 mAb,while there was HSP70 in the immunoprecipitate of antiAFP mAb.CONCLUSION: HSP70 chaperones AFP in human HCCcell BEL-7402. The interaction between HSP70 and AFP in human HCC cell can be a new route to study the pathogenesis and immunotherapy of HCC.

  15. Cytological alteration of cultured rat liver cells by 3'-methyl-4-dimethylaminoazobenzene with special reference to chromosome changes, changes of growth patterns at a colony level and alpha-fetoprotein production.

    Directory of Open Access Journals (Sweden)

    Tokiwa,Takayoshi

    1980-06-01

    Full Text Available A near diploid clone derived from a rat liver cell line was continuously treated with various concentrations of 3'-methyl-4-dimethylaminoazobenzene (3'-Me-DAB in culture. By treatment with 2.8 micrograms/ml, cells with 41 chromosomes formed a mode and which then shifted to 39. The chromosome numbers of cells treated with 5.4 micrograms/ml were widely distributed at early stages, but later the mode shifted to hypotetraploid region. Untreated control cells were confirmed as near diploid. Increased plating efficiency by 3'-Me-DAB as well as the appearance of large sized colonies was obtained. The production of alpha-fetoprotein (AFP by the cells was slightly enhanced by treatment with 3'-Me-DAB. The cells treated with and without 3'-Me-DAB did not produce any tumor in rats 6 months after their intraperitoneal injection.

  16. Solitary pancreas metastasis from AFP-producing gastric cancer: report of a case.

    Science.gov (United States)

    Natsume, Toshiyuki; Watanabe, Yoshiji; Maruyama, Takashi; Tanaka, Hajime; Shimizu, Takanori; Saito, Tsuyoshi; Kobayashi, Yutaka; Tohnosu, Noriyuki; Uehara, Toshitaka; Shimizu, Shin-ichiro; Ochiai, Takenori

    2005-01-01

    We herein present a case of resected pancreatic metachronous metastasis arising from alpha-fetoprotein-producing gastric cancer. A 75-year-old man underwent distal gastrectomy for alpha-fetoprotein-producing gastric cancer in November 1999. The staging group of TNM classification was Stage IIIA. The serum AFP level normalized after surgical resection. During the follow-up period, it increased to 42ng/mL in January 2002, and up to 550ng/mL in July 2002: Abdominal computed tomography disclosed a 4-cm mass in the tail of the pancreas and under the diagnosis of pancreas metastasis, distal pancreatectomy with splenectomy was performed. Following this, serum alpha-fetoprotein declined to 61ng/mL, and the postoperative course was uneventful. But it elevated again to 200ng/mL in August, and to 3500ng/mL in September 2002. Computed tomography revealed multiple liver metastases. He was treated with TS-1, but hepatic lesions continued to grow and he died in March 2003. To our knowledge, this case is the first report of resection of solitary pancreas metastasis of alpha-fetoprotein-producing gastric cancer. PMID:16001678

  17. Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells

    Institute of Scientific and Technical Information of China (English)

    Yun-Shan Wang; Xiao-Li Ma; Tong-Gang Qi; Xiang-Dong Liu; Yue-Sheng Meng; Guang-Ju Guan

    2005-01-01

    AIM: To study the function of α-fetoprotein (AFP) in SMMC-7721 hepatoma cells.METHODS: A hairpin siRNA expressing plasmid pSilencer3.0-H1-afp was constructed and transfected into SMMC-7721 cells with Lipofectamine 2000. The expression of AFP was monitored by real-time RT-PCR and immunoassays, its effect on SMMC-7721 cell proliferation and cell death was detected by MTT and fluorescenceactivated cell sorter (FACS).RESULTS: The AFP-siRNA expressing plasmid downregulated the expression of AFP obviously (about 34%), and inhibited SMMC-7721 cell proliferation, but did not induce apoptosis.CONCLUSION: Downregulation of AFP siRNA inhibits proliferation of SMMC-7721 cells, but cannot cause apoptosis.

  18. Antitumor immunity induced by DNA vaccine encoding alpha-fetoprotein/heat shock protein 70

    Institute of Scientific and Technical Information of China (English)

    Xiao-Ping Wang; Guo-Zhen Liu; Ai-Li Song; Hai-Yan Li; Yu Liu

    2004-01-01

    AIM: To construct a DNA vaccine encoding human alphafetoprotein (hAFP)/heat shock protein 70 (HSP70), and to study its ability to induce specific CTL response and its protective effect against AFP-expressing tumor.METHODS: A DNA vaccine was constructed by combining hAFP gene with HSP70 gene. SP2/0 cells were stably transfected with pBBS212-hAFP and pBBS212-hAFP/HSP70eukaryotic expression vectors. Mice were primed and boosted with DNA vaccine hAFP/HSP70 by intramuscular injection, whereas plasmid with hAFP or HSP70 was used as controls. ELISPOT and ELISA were used to detect IFN-γ-producing splenocytes and the level of serum anti-AFP antibody from immunized mice respectively. In vivo tumor challenge was measured to assess the immune effect of the DNA vaccine.RESULTS: By DNA vaccine immunization, the results of ELISPOT and ELISA showed that the number of IFN-γ-producing splenocytes and the level of serum anti-AFP antibody were significantly higher in rhAFP/HSP70 group than in hAFP and empty plasmid groups (95.50±10.90IFN-γ spots/106 cells vs 23.60±11.80 IFN-γ spots/106 cells,7.17±4.24 IFN-γ spots/106 cells, P<0.01; 126.50±8.22 μg/mL vs 51.72±3.40 μg/mL, 5.83±3.79 μg/mL, P<0.01). The tumor volume in rhAFP/HSP70 group was significantly smaller than that in pBBS212-hAFP and empty plasmid groups (37.41±7.34 mm3 vs381.13±15.48 mm3, 817.51±16.25 mm3,P<0.01).CONCLUSION: Sequential immunization with a recombinant DNA vaccine encoding AFP and heat shock protein70 could generate effective AFP-specific T cell responses and induce definite antitumor effects on AFP-producing tumors, which may be suitable for some clinical testing as a vaccine for HCC.

  19. Ovarian Sertoli-Leydig cell tumor with heterologous elements of gastrointestinal type associated with elevated serum alpha-fetoprotein level: an unusual case and literature review

    OpenAIRE

    Horta, Mariana; Cunha, Teresa Margarida; Marques, Rita Canas; Félix, Ana

    2014-01-01

    Here we describe the case of a 19-year-old woman with a poorly differentiated ovarian Sertoli-Leydig cell tumor and an elevated serum alpha-fetoprotein level. The patient presented with diffuse abdominal pain and bloating. Physical examination, ultrasound, and magnetic resonance imaging revealed a right ovarian tumor that was histopathologically diagnosed as a poorly differentiated Sertoli-Leydig cell tumor with heterologous elements. Her alpha-fetoprotein serum level was undetectable after t...

  20. Electrochemical immunosensor for alpha-fetoprotein determination based on ZnSe quantum dots/Azure I/gold nanoparticles/poly (3,4-ethylenedioxythiophene) modified Pt electrode

    International Nuclear Information System (INIS)

    A novel amperometric enzyme immunosensor with amplified sensitivity for the determination of alpha-fetoprotein (AFP) was constructed with layer-by-layer assembly of ZnSe quantum dots (ZnSe QDs)/Azure I/gold nanoparticles (nanoAu)/poly (3,4-ethylenedioxythiophene) (PEDOT) on Pt electrode. Firstly, citrate coated nanoAu was immobilized on the PEDOT polymer film, which was electrochemically synthesized in ionic liquid electrolyte of 1-butyl-3-methylimidazolium tetrafluoroborate ([bmim][BF4]). Then, Azure I was immobilized on nanoAu/PEDOT composite matrix as a redox probe and was used to immobilize ZnSe QDs. Subsequently, AFP antibody (anti-AFP) was adsorbed onto the surface of ZnSe layer. Finally, horseradish peroxidase (HRP) was employed to block sites against nonspecific binding and amplify the current signal of the antigen–antibody reaction. The modification processes were characterized by cyclic voltammetry, scanning electron microscopy. The factors influenced the performances of the proposed immunosensors were studied in detail. Because of the synergism between Azure I and nanoAu/PEDOT to facilitate electron-transfer process, and the small diameter of ZnSe QDs favorable for stabilization of biological activity to a large extent, the immunosensor displayed a high sensitivity, fast analytical time, a relatively low detection limit of 1.1 fg/mL at 3 times of signal-to-noise ratio (S/N = 3), and a especially broad linear response to AFP in a ranges from 5 × 10−5 to 250 ng/mL. Moreover, the selectivity, repeatability, and stability of the proposed immunosensor were acceptable

  1. Diagnosis value of alpha-fetoprotein heterogeneity in primary hepatic carcinoma%甲胎蛋白异质体在原发性肝癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    陈俊; 王利健; 骆剑明; 吴荣辉; 楼跃民; 朱笑频

    2012-01-01

    目的:探讨甲胎蛋白异质体在肝脏良恶性疾病诊断中的意义.方法:采用微量离心柱法对240例肝病患者(原发性肝癌70例,肝癌术后10例,肝硬化63例,慢性肝炎97例),采用化学发光法检测AFP及AFP-L3水平,计算AFP-L3和AFP比例.结果:原发性肝癌组患者血清中的AFP,AFP-L3%阳性率显著高于肝癌术后组,肝硬化组和慢性肝炎组.AFP-L3%的灵敏度为86.96%,特异性为88.30%,Youden指数为0.75.AFP的灵敏度为75.68%,特异性为85.54%,Youden指数为0.61.从Youden指数可以知道AFP-L3%是比AFP更好的诊断肝癌的指标.原发性肝癌组患者血清中的AFP、AFP-L3%含量显著高于肝癌术后组、肝硬化组和慢性肝炎组(F值分别为74.45、54.85,P值均<0.01).诊断为原发性肝癌病人中,当AFP> 400 ng/ml时,AFP-L3%的阳性率为95.23%.AFP-L3%与AFP水平无相关性.结论:AFP-L3%对于肝癌的诊断有较高的灵敏性和特异性;是比AFP更好的诊断指标;可以作为诊断肝癌的独立指标.%Objective: To explore the significance of alpha - fetoprotein heterogeneity (AFP - L3) in differential diagnosis of malignant liver disease. Methods: Serum samples were collected from 240 patients, 70 cases with hep-atocellular carcinoma, 10 cases with postoperative liver cancer, 63 cases with cirrhosis, 97 cases with chronic hepatitis. AFP - L3 was isolated by microspincolumn with lens culinaris agglutinin. The levels of AFP and AFP - L3 were detected by chemiluminescence method. Results: The ratios of AFP and AFP - 13% in hepatocellular carcinoma group were significantly higher those in hepatectomy group, liver cirrhosis and chronic hepatitis group (χ2 values were 87. 30, 142.22, P <0. 01 ). For AFP - L3% , sensitivity was 86. 96% , specificity was 88. 30% , and the Youden index was 0. 75, while for AFP, sensitivity was 75. 68% , specificity was 85. 54% , Youden index was 0. 61. From the Youden index, it can be seen the AFP - L3

  2. Relapse surveillance in AFP-positive hepatoblastoma: re-evaluating the role of imaging

    Energy Technology Data Exchange (ETDEWEB)

    Rojas, Yesenia; Vasudevan, Sanjeev A.; Nuchtern, Jed G. [Baylor College of Medicine, Pediatric Surgery Division, Michael E. DeBakey Department of Surgery, Texas Children' s Hospital, Houston, TX (United States); Guillerman, R.P. [Baylor College of Medicine, Department of Pediatric Radiology, Texas Children' s Hospital, Houston, TX (United States); Zhang, Wei [Texas Children' s Hospital, Surgical Outcomes Center, Houston, TX (United States); Thompson, Patrick A. [Baylor College of Medicine, Hematology-Oncology Division, Department of Pediatrics, Texas Children' s Cancer Center, Texas Children' s Hospital, Houston, TX (United States); University of North Carolina, Hematology-Oncology Division, Department of Pediatrics, North Carolina Children' s Hospital, Chapel Hill, NC (United States)

    2014-10-15

    Children with hepatoblastoma routinely undergo repetitive surveillance imaging, with CT scans for several years after therapy, increasing the risk of radiation-induced cancer. The purpose of this study was to determine the utility of surveillance CT scans compared to serum alpha-fetoprotein (AFP) levels for the detection of hepatoblastoma relapse. This was a retrospective study of all children diagnosed with AFP-positive hepatoblastoma from 2001 to 2011 at a single institution. Twenty-six children with hepatoblastoma were identified, with a mean age at diagnosis of 2 years 4 months (range 3 months to 11 years). Mean AFP level at diagnosis was 132,732 ng/ml (range 172.8-572,613 ng/ml). Five of the 26 children had hepatoblastoma relapse. A total of 105 imaging exams were performed following completion of therapy; 88 (84%) CT, 8 (8%) MRI, 5 (5%) US and 4 (4%) FDG PET/CT exams. A total of 288 alpha-fetoprotein levels were drawn, with a mean of 11 per child. The AFP level was elevated in all recurrences and no relapses were detected by imaging before AFP elevation. Two false-positive AFP levels and 15 false-positive imaging exams were detected. AFP elevation was found to be significantly more specific than PET/CT and CT imaging at detecting relapse. We recommend using serial serum AFP levels as the preferred method of surveillance in children with AFP-positive hepatoblastoma, reserving imaging for the early postoperative period, for children at high risk of relapse, and for determination of the anatomical site of clinically suspected recurrence. Given the small size of this preliminary study, validation in a larger patient population is warranted. (orig.)

  3. Effects of alpha fetoprotein on escape of Bel 7402 cells from attack of lymphocytes

    International Nuclear Information System (INIS)

    Involvement of AFP against apoptosis of tumor cell has been implicated in its evasion of immune surveillance. However, the molecular events of immune escape mechanisms are still unknown. The major observations reported here relate to a possible mechanism by which heptoloma Bel 7402 cells escape immune surveillance in vitro. Western blotting and a well-characterized cofocal scanning image were performed to analyze the expression of Fas/FasL and caspase-3 in co-cultured Bel 7402 and Jurkat cells. After co-culture with Jurkat cells, up-regulated Fas and reduced FasL expression could be observed. Treatment with AFP could remarkably inhibit the elevated Fas and, whereas, induce the FasL expression in co-cultured Bel 7402 cells. Cells co-culture could induce the expression of caspase-3 in both cells line. The elevated caspase-3 in Bel 7402 cells was abolished following the treatment of AFP. The expression of caspase-3 was elevated in co-cultured Jurkat cells treated with AFP. No detectable change on the expression of survivin was examined in both cells line. Monoclonal antibody against AFP treatment alone did not obviously influence the growth of cells, as well as the expression of Fas/FasL and caspase-3. However, the effect of AFP could be blocked by antibody. our results provide evidence that AFP could promote the escape of liver cancer cells from immune surveillance through blocking the caspase signal pathway of tumor cells and triggering the Fas/FasL interaction between tumor cells and lymphocytes

  4. [Alpha fetoprotein and neonatal jaundice. Contribution to the study of a physiopathologic mechanism].

    Science.gov (United States)

    Tourne, C E; Brettes, J P; Guez, G; Ritter, J; Gandar, R

    1977-01-01

    The increase in the maternal plasma A.F.P. level is due to an hypoxia of the foetus. The prospective study of 851 single pregnancies shows that there is a significant rise in the A.F.P. levels during the last days of the pregnancy if the babies are going to exhibit a so-called physiological jaundice at birth: the decrease of the A.F.P. levels in these cases is four times slower than in normal cases. The prospective study of another group of 404 pregnancies gave the same results for the A.F.P. level of the blood of the umbilical cord. Statistical analysis showed that the pathological conditions capable of increasing the A.F.P. levels are related to neo-natal jaundice. The neo-natal jaundice may be due to a factor of foetal hypoxia capable of inducing an over stimulation of the foetal erythropoiesis. The results of this mechanism would be a quantitative disequilibrium between an increased hemolysis and a reduced bilirubine fixation capacity during the neo-natal period.

  5. The in Vitro Assessment of Biochemical Factors in Hepatocyte like Cells Derived from Umbilical Cord Blood Stem Cells

    Directory of Open Access Journals (Sweden)

    A KHoramroodi

    2009-10-01

    Full Text Available Introduction & Objective: Umbilical cord blood (UCB is a source of Hematopoietic Stem Cells (HSC and progenitor cells that can reconstitute the hematopoietic system in patients with malignant and nonmalignant disorders. Mesenchymal stem cell-derived from umbilical cord blood (UCB have been differentiated to some kind of cells, such as osteobblast, adipoblast and chondroblast in Vitro. This study examined the differentiation of Umbilical Cord Blood (UCB derived stem cells to functional hepatocytes. Materials & Methods: The present study was an experimental study which was carried out in the Payam-e-Noor University of Tehran in cooperation with Hamedan University of Medical Sciences in 2008. Umbilical cord blood (UCB was obtained from Fatemieh hospital (Hamadan, Iran. Stem cells were isolated from the cord blood by combining density gradient centrifugation with plastic adherence. When the isolated cells reached 80% confluence, they differentiated to hepatocyte like cells. The medium which was used was consists of DMEM and 10% Fetal Bovine Serum (FBS supplemented with 20 ng/mL Hepatocyte Growth Factor (HGF, 10 ng/mL basic Fibroblast Growth Factor (bFGF and 20 ng/mL Oncostatin M (OSM.The medium was changed every 3 days and stored for Albumin (ALB, Alpha Fetoprotein (AFP, Alkaline Phosphatase (ALP, and urea assay. Finally PAS stain was done to study Glycogen storage in the differentiated cell. Results: Measurement of biochemical factors in different days showed that concentration of albumin (ALB, alpha fetoprotein (AFP, alkaline phosphatase (ALP, and Urea gradually increased. Also, PAS staining showed the storage of glycogen in these cells. Conclusion: Stem cell-derived from human umbilical cord blood (HUCB is a new source of cell types for cell transplantation therapy of hepatic diseases and under certain conditions these cells can differentiate into liver cells.

  6. Pregnant women's attitudes for accepting or declining a serum-alpha-fetoprotein test.

    Science.gov (United States)

    Sandén, M L; Bjurulf, P

    1988-01-01

    This study analyzes attitudes which influence women in their decisions to accept or decline a serum-Alpha-FetoProtein (se-AFP) test. The survey covers all women who have visited antenatal clinics offering the se-AFP test in a given area during a specified period. All in all, the pregnant women answered four questionnaires: three before and one after delivery. 19% of the women declined the se-AFP test. During pregnancy, the women who did not take the se-AFP test said that the reason was anxiety about the results of the test. Many of the women who took the test said that they wanted assurance that the child was healthy. This entails obvious ethical problems when the test is positive. The test-routine does not have a major influence in the decision-making process. A change in routines to shorten the waiting time, and a different cut-off line might influence some of the declining women to accept the test.

  7. 孕中期异常甲胎蛋白与不良妊娠结局的相关性%The association between second - trimester maternal serum abnormal alpha - fetoprotein and adverse pregnancy outcome

    Institute of Scientific and Technical Information of China (English)

    吴满武; 俞信忠; 杨志浩; 张涛; 罗婷婷

    2012-01-01

    目的 探讨孕中期甲胎蛋白(AFP)与不良妊娠结局的相关性,为妊娠管理提供建议.方法 对孕中期14 008例孕妇进行唐氏筛查.研究组:①AFP MoM>2.0孕妇338例.②AFP MoM<0.5孕妇224例.依据AFP MoM值不同分组.对照组:AFP MoM值都在正常范围内的孕妇318例.回顾分析妊娠结局资料.结果 ①AFP MoM>2.0的孕妇发生出生缺陷(ρ<0.01)的机会明显比正常孕妇高.②AFP MoM> 3.5的孕妇发生出生缺陷(P<0.001)和早产(P<0.05)的机会明显比正常孕妇高.③AFP MoM> 5.0的孕妇发生出生缺陷(P<0.001)、早产(P<0.01)和胎儿窘迫(P<0.05)的机会明显比正常孕妇高.④AFP MoM<0.5和<0.25的孕妇均未发现与不良妊娠结局相关.结论 异常水平的标志物除了能够提供开放性神经管缺损风险外,还能提供不良妊娠结局的风险信息,而这些信息对产前遗传咨询和妊娠管理是非常重要.%Objective; Aim of this study is to determine the association between risk of adverse pregnancy outcome and abnormal maternal serum alpha - fetoprotein in second trimester screening. Abnormal levels of maternal serum markers provide information which is important for counseling and pregnancy management. Methods: A total of 14 008 pregnant women underwent maternal serum Down' s syndrome screening. Study groups include:①338 cases were found to have a serum AFP of > 2.0 multiples of the median ( MoM). ②cases serum were found to have a serum AFP 2.0 MoM have higher occurrence of birth defects (P 3. 5 MoM have higher occurrence of birth defects ( P 5.0 MoM have higher occurrence of birth defects (P < 0. 001) and preterm labor ( P < 0. 01) and fetal stress ( P < 0. 05). ④Pregnant women with a serum AFP of < 0. 5 M < 0. 25 MoM were found to have no association with adverse outcomes. Conclusion: Abnormal levels of maternal serum markers can provide risk information of adverse outcomes in addition to the risks for open neural tube defects

  8. Gastric Composite Tumor of Alpha Fetoprotein-Producing Carcinoma/Hepatoid Adenocarcinoma and Endocrine Carcinoma with Reference to Cellular Phenotypes

    Directory of Open Access Journals (Sweden)

    Akira Suzuki

    2012-01-01

    Full Text Available Alpha-fetoprotein-producing carcinoma (AFPC/hepatoid adenocarcinoma (HAC and neuroendocrine carcinoma (NEC are uncommon in the stomach. Composite tumors consisting of these carcinomas and their histologic phenotypes are not well known. Between 2002 and 2007, to estimate the prevalence of composite tumors consisting of tubular adenocarcinoma, AFPC/HAC and NEC, we reviewed specimens obtained from 294 consecutive patients treated surgically for gastric cancer. We examined histological phenotype of tumors of AFPC or NEC containing the composite tumor by evaluating immunohistochemical expressions of MUC2, MUC5AC, MUC6, CDX2, and SOX2. Immunohistochemically, AFPC/HAC dominantly showed the intestinal or mixed phenotype, and NEC frequently showed the gastric phenotype. In the composite tumor, the tubular and hepatoid components showed the gastric phenotype, and the neuroendocrine component showed the mixed type. The unique composite tumor predominantly showed the gastric phenotype, and the hepatoid and neuroendocrine components were considered to be differentiated from the tubular component.

  9. Alpha-1-antitrypsin deficiency resulting in a hitherto unseen presentation of hepatocellular carcinoma: Polycythemia but with normal alpha fetoprotein

    Institute of Scientific and Technical Information of China (English)

    David Ryan Owen; Ramachandran Sivakumar; Eui-Sik Suh; Murugiah Seevaratnam

    2006-01-01

    Polycythemia is a known paraneopastic manifestation of hepatoma, but only in the presence of alpha-fetopro (AFP). We present a case of polycythemia in the absence of AFP, and suggest concurrent alpha-1-antitrypsin deficiency as the cause for breaking this rule. We also suggest a reason for the apparent constant conjunction between polycythemia and AFP in hepatoma.

  10. CRB2 mutations produce a phenotype resembling congenital nephrosis, Finnish type, with cerebral ventriculomegaly and raised alpha-fetoprotein.

    Science.gov (United States)

    Slavotinek, Anne; Kaylor, Julie; Pierce, Heather; Cahr, Michelle; DeWard, Stephanie J; Schneidman-Duhovny, Dina; Alsadah, Adnan; Salem, Fadi; Schmajuk, Gabriela; Mehta, Lakshmi

    2015-01-01

    We report five fetuses and a child from three families who shared a phenotype comprising cerebral ventriculomegaly and echogenic kidneys with histopathological findings of congenital nephrosis. The presenting features were greatly elevated maternal serum alpha-fetoprotein (MSAFP) or amniotic fluid alpha-fetoprotein (AFAFP) levels or abnormalities visualized on ultrasound scan during the second trimester of pregnancy. Exome sequencing revealed deleterious sequence variants in Crumbs, Drosophila, Homolog of, 2 (CRB2) consistent with autosomal-recessive inheritance. Two fetuses with cerebral ventriculomegaly and renal microcysts were compound heterozygotes for p.Asn800Lys and p.Trp759Ter, one fetus with renal microcysts was a compound heterozygote for p.Glu643Ala and p.Asn800Lys, and one child with cerebral ventriculomegaly, periventricular heterotopias, echogenic kidneys, and renal failure was homozygous for p.Arg633Trp in CRB2. Examination of the kidneys in one fetus showed tubular cysts at the corticomedullary junction and diffuse effacement of the epithelial foot processes and microvillous transformation of the renal podocytes, findings that were similar to those reported in congenital nephrotic syndrome, Finnish type, that is caused by mutations in nephrin (NPHS1). Loss of function for crb2b and nphs1 in Danio rerio were previously shown to result in loss of the slit diaphragms of the podocytes, leading to the hypothesis that nephrosis develops from an inability to develop a functional glomerular barrier. We conclude that the phenotype associated with CRB2 mutations is pleiotropic and that the condition is an important consideration in the evaluation of high MSAFP/AFAFP where a renal cause is suspected.

  11. Risk factors for hepatocellular carcinoma may impair the performance of biomarkers: a comparison of AFP, DCP, and AFP-L3.

    Science.gov (United States)

    Volk, Michael L; Hernandez, Jose C; Su, Grace L; Lok, Anna S; Marrero, Jorge A

    2007-01-01

    Current surveillance strategies for hepatocellular carcinoma (HCC) are applied uniformly in patients with cirrhosis, regardless of their cancer risk. The aim of this study was to compare the performance characteristics of the biomarkers alpha-fetoprotein (AFP), des-gamma carboxyprothrombin (DCP), and lectin-bound AFP (AFP-L3) in the diagnosis of HCC, and to determine the effect of risk factors for HCC on test performance. Eighty-four patients with HCC and 169 patients with cirrhosis were enrolled and their serum analyzed for total AFP, AFP-L3 and DCP. Receiver-operating characteristic (ROC) curves were constructed to determine the performance characteristics. DCP was significantly better than total AFP or AFP-L3 in differentiating HCC from cirrhosis, with a sensitivity of 86% and specificity of 93%. When subjects were divided into two groups by their risk for HCC, all 3 markers had a lower sensitivity and area under the ROC curve in the high-risk group compared to the low-risk group. In conclusion, DCP has the best performance characteristics of all 3 serum markers for the diagnosis of HCC. Serum biomarkers may be less sensitive and specific in the highest risk patients. PMID:17522429

  12. Risk factors for hepatocellular carcinoma may impair the performance of biomarkers: a comparison of AFP, DCP, and AFP-L3.

    Science.gov (United States)

    Volk, Michael L; Hernandez, Jose C; Su, Grace L; Lok, Anna S; Marrero, Jorge A

    2007-01-01

    Current surveillance strategies for hepatocellular carcinoma (HCC) are applied uniformly in patients with cirrhosis, regardless of their cancer risk. The aim of this study was to compare the performance characteristics of the biomarkers alpha-fetoprotein (AFP), des-gamma carboxyprothrombin (DCP), and lectin-bound AFP (AFP-L3) in the diagnosis of HCC, and to determine the effect of risk factors for HCC on test performance. Eighty-four patients with HCC and 169 patients with cirrhosis were enrolled and their serum analyzed for total AFP, AFP-L3 and DCP. Receiver-operating characteristic (ROC) curves were constructed to determine the performance characteristics. DCP was significantly better than total AFP or AFP-L3 in differentiating HCC from cirrhosis, with a sensitivity of 86% and specificity of 93%. When subjects were divided into two groups by their risk for HCC, all 3 markers had a lower sensitivity and area under the ROC curve in the high-risk group compared to the low-risk group. In conclusion, DCP has the best performance characteristics of all 3 serum markers for the diagnosis of HCC. Serum biomarkers may be less sensitive and specific in the highest risk patients.

  13. Prognostic value of the content of lentil lectin-rcactive alpha-fetoprotein-L3 in early-stage hepatocellular carcinoma%甲胎蛋白异质体3含量对早期肝癌的预后价值

    Institute of Scientific and Technical Information of China (English)

    冯凯; 马宽生; 高峻; 刘念洲; 王曙光

    2008-01-01

    目的 探讨甲胎蛋白异质体3(lentil lectin-reactive alpha-fetoprotein-L3,AFP-L3)的含量对早期肝癌的预后价值.方法 97例早期肝癌患者根据术前AFP和AFP-13的含量分为:(1)AFP阳性、AFP-L3阴性组(29例):AFP>20 μg/L & AFP-13<15%;(2)AFP-L3、AFP均低含量组(16例):15%≤AFP-L3≤50% & 20 μg/L≤AFP≤200 μg/L;(3)AFP-L3、AFP均高含量组(13例):AFP-L3>50% & AFP>200 μg/L;(4)AFP-L3高含量、AFP低含量组(24例):AFP-13>50%& 20 μg/L≤AFP≤200 μg/L;(5)AFP-L3低含量、AFP高含量组(15例):15%≤AFP-L3≤50% & AFP>200 μg/L.对各组患者的肿瘤分化程度、术后1、2、3年生存率和无瘤生存率进行分析.结果 AFP-13阴性患者的肿瘤分化程度、术后3年生存率和无瘤牛存率明显优于AFP-L3阳性患者(χ2=21.051,10.043,4.450,6.977,25.566,P<0.05).AFP-L3高含量组患者的肿瘤分化程度、术后1、2、3年生存率和无瘤生存率明显低于低含量组(χ2=7.938,3.488,9.085,P<0.05).结论 AFP-L3含量的增高提示肿瘤恶性程度高,预后不良,尤其是AFP水平低时.手术前后检测AFP-L3含量对于患者预后的评价具有指导意义.%Objective To explore the prognostic value of the content of lentil lectin-reactive alphafetoprotein-L3(AFP-L3)in early-stage hepatocellular carcinoma(HCC).Methods According to the content of alpha.fetoprotein(AFP)and AFP-L3 before the treatment,97 patients with early-stage HCC were divided into group A(AFP>20 μg/L & AFP-L3<15%,n=29),group B(15%≤AFP-13≤50% & 20 μg/L≤AFP≤200 μg/L,n=16),group C(AFP-13>50%& AFP>200 μg/L,n=13),group D(AFP-L3>50% & 20 μg/L≤AFP≤200 μg/L,n=24)and group E(15%≤AFP-L3≤50%& AFP>200 μg/L,n=15).The degree of tumor differentiation,the 1-,2-,3-year survival rates and tumor-free survival rates of the patients were analyzed.Results The degree of tumor differentiation.3-year survival rate and tumor-free survival rate of patients in group A were significantly higher than

  14. 血清甲胎蛋白异质体3测定在原发性肝癌早期筛查中作用%Value of serum alpha-fetoprotein detection in early screening of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    周云龙; 廖志军; 邓国孙; 丁罡

    2014-01-01

    Objective To explore the value of serum alpha-fetoprotein (AFP-L3) in early screening of primary liver cancer. Methods Lentil lectin coupled with ( LCA) micro-centrifuge column was separated to obtain AFP-L3 from 116 cases of chronic liver disease and primary liver cancer .AFP before separation and AFP-L3 after separation were detected with chemoluminescence , and their levels were then calculated .Results Statistical differences could be seen in the average value of AFP-L3 between the patients with pri-mary liver cancer and patients with chronic liver disease (P<0.01).Conclusion The level detected with micro-centrifuge column was of importance in early screening of primary liver cancer .%目的:探讨血清甲胎蛋白异质体3(AFP-L3)在原发性肝癌早期筛查中的作用。方法以装有耦联小扁豆凝集素(LCA)的微量离心柱分离慢性肝病、原发性肝癌共116例患者AFP-L3,用化学发光方法检测分离前后AFP-L3,计算AFP-L3在AFP中的含量比例。结果原发性肝癌组AFP-L3含量比例均值[(36.89±2.13)%],与慢性肝病组[(7.45±2.32)%]相比差异有统计学意义( P<0.01)。结论微量离心柱法测定AFP-L3的含量在原发性肝癌的早期筛查中具有重要意义。

  15. 三种肿瘤标志物联合检测在原发性肝细胞癌中的应用价值%Value of combined detection of α-L-fucosidase,sialie acid and alpha-fetoprotein for diagnosis of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    王娟; 刘寓; 李莲

    2011-01-01

    Aim To detect the serum concentrations of Alpha-fetoprotein(AFP),α-L-fucosidase(AFU)and sialic acid (SA)for diagnosis of hepatocellular carcinoma.Methods The levels of AFP,AFU and SA in 47 confirmed primary hepatocellular carninoma cases,45 cirrhosis cases and 42 healthy controls were determined with chemiluminescent enzyme immunoassay.Results The diagnostic rate could enhanced by 97.87% through combined detection of the levels of AFP, AFU and SA.Conclusion The positive rate of diagnosis of primary hepatocellular carcinoma can obviously enhanced by joint detection of AFP, AFU and SA, and it is suitable for clinical application.%目的 探讨联合检测患者血清中的甲胎蛋白(AFP)、α-L-岩藻糖苷酶(AFU)以及唾液酸(SA)时原发性肝细胞癌(PHC)诊断的应用价值.方法 采用化学发光酶免疫法、速率法和分光光度法,分别检测原发性肝细胞癌患者、肝硬化和健康对照者的AFP、AFU和SA等三种肿瘤标志物的血清含量,并进行统计学分析.结果 AFP、AFU、SA 三者联合检测、可以将诊断率提高到97.87%.结论 三种肿瘤标志物AFP、α-L-AFU、SA多指标联合检测可显著提高原发性肝细胞癌(PHC)诊断阳性率,适宜于临床实验室推广应用.

  16. An Ultrasensitive Electrochemical Immunosensor for Alpha-Fetoprotein Using an Envision Complex-Antibody Copolymer as a Sensitive Label

    Directory of Open Access Journals (Sweden)

    Lei Zheng

    2012-12-01

    Full Text Available A novel strategy is presented for sensitive detection of alfa-fetoprotein (AFP, using a horseradish peroxidase (HRP-functionalized Envision antibody complex (EVC as the label. The Envision-AFP signal antibody copolymer (EVC-AFP Ab2 was composed of a dextran amine skeleton anchoring more than 100 molecules of HRP and 15 molecules of secondary antibody, and acted as a signal tag in the immunosensor. The sensor was constructed using the following steps: First, gold electrode (GE was modified with nano-gold (AuNPs by electro-deposition in HAuCl4 solution. The high affinity of the AuNPs surface facilitates direct formation of a self-assembled thiolated protein G layer. Next, the coated GE was incubated in a solution of AFP capture antibody (AFP Ab1; these antibodies attach to the thiolated protein G layer through their non-antigenic regions, leaving the antigen binding sites for binding of target analyte. Following a sandwich immunoreaction, an EVC-AFP Ab2-AFP-AFP Ab1 immunocomplex was formed on the electrode surface, allowing large amounts of HRP on the complex to produce an amplified electrocatalytic current of hydroquinone (HQ in the presence of hydrogen peroxide (H2O2. Highly amplified detection was achieved, with a detection limit of 2 pg/mL and a linear range of 0.005–0.2 ng/mL for AFP in 10 μL undiluted serum; this is near or below the normal levels of most cancer biomarker proteins in human serum. Measurements of AFP in the serum of cancer patients correlated strongly with standard enzyme-linked immunosorbent assays. These easily fabricated EVC-modified immunosensors show excellent promise for future fabrication of bioelectronic arrays. By varying the target biomolecules, this technique may be easily extended for use with other immunoassays, and thus represents a versatile design route.

  17. Clinical evaluation of radioassays for calcitonin, CEA-S, AFP and ferritin as tumor markers

    International Nuclear Information System (INIS)

    Clinical usefulness of the radioassays for serum calcitonin, isometric species of carcinoembryonic antigen (CEA-S), alpha-fetoprotein (AFP) and ferritin as the specific tumor markers was evaluated. The calcitonin assay was found to be extremely useful in detecting the cases of familial medullary carcinoma of the thyroid and in monitoring the patients after surgery. The CEA-S assay was proved more specific in titers and positivity for malignancies, especially GI tract carcinomas than CEA assay. The diagnosis of hepatoma could be performed precisely with a combination of 67Ga scintigraphy and assays of CEA and AFP. The serum ferritin levels in malignancies overlapped widely with those of non-malignancies. However, the estimation of ferritin-iron ratio was thought to be a useful means for screening patients with suspicious lesions. (author)

  18. Peri-Transplant Change in AFP Level: a Useful Predictor of Hepatocellular Carcinoma Recurrence Following Liver Transplantation.

    Science.gov (United States)

    Yoo, Tae; Lee, Kwang-Woong; Yi, Nam-Joon; Choi, Young Rok; Kim, Hyeyoung; Suh, Suk-Won; Jeong, Jae Hong; Lee, Jeong-Moo; Suh, Kyung-Suk

    2016-07-01

    Pretransplant alpha-fetoprotein (AFP) is a useful tumor marker predicting recurrence of hepatocellular carcinoma (HCC). Little is known, however, about the relationship between changes in AFP concentration and prognosis. This study investigated the clinical significance of change in peri-transplant AFP level as a predictor of HCC recurrence. Data from 125 HCC patients with elevated pretransplant AFP level who underwent liver transplantation (LT) between February 2000 and December 2010 were retrospectively reviewed. Patients with AFP normalization within 1 month after LT were classified into the rapid normalization group (n = 97), with all other patients classified into the non-rapid normalization group (n = 28). Tumor recurrence was observed in 17 of the 97 patients (17.5%) with rapid normalization; of these, 11 patients had high AFP levels and six had normal levels at recurrence. In contrast, tumor recurrence was observed in 24 of the 28 patients (85.7%) without rapid normalization, with all 24 having high AFP levels at recurrence. Multivariate analysis showed that non-rapid normalization (harzard ratio [HR], 4.41, P AFP level at 1 month is a useful clinical marker for HCC recurrence. Therefore, an adjuvant strategy and/or intensive screening are needed for patients who do not show rapid normalization. PMID:27366001

  19. 血清甲胎蛋白、a-L-岩藻糖苷酶、al-抗胰蛋白酶在原发性肝癌诊断中的应用%The application of serum alpha fetoprotein,a-L-fucosidase and alphao-antitrypsin detection in diagnosis of primary hepatic carcinoma

    Institute of Scientific and Technical Information of China (English)

    权翠侠; 王庆国; 滕晓梅

    2008-01-01

    目的 探讨甲胎蛋白(alpha fetoprotein,AFP)、a-L-岩藻糖苷酶(a-L-fucosidase,AFU)、al-抗胰蛋白酶(alphao-antitrypsin,AAT)在原发性肝癌(PHC)患者诊断中的应用价值.方法 选择126例原发性肝癌患者、397例良性肝病患者以及206名健康对照者,应用化学发光法测定AFP,新型CNPF(2-氯-4-硝基苯-a-L-岩藻吡喃糖苷)连续监测法测定AFU,速率散射比浊法测定AAT.结果 原发性肝癌组AFP、AFU、APT与良性肝痛组及健康对照组差异有显著性(P<0.01),单独检测对PHC诊断的阳性率分别为73.8%、85.7%、84.1%,三者联合检测可将PHC诊断阳性率提高到96%.结论 血清AFU、AAT、与AFP互为补充,联合检测可提高对PHC的诊断率.

  20. Hepatocytic differentiation of mesenchymal stem cells in cocultures with fetal liver cells

    Institute of Scientific and Technical Information of China (English)

    Claudia Lange; Helge Bruns; Dietrich Kluth; Axel R Zander; Henning C Fiegel

    2006-01-01

    AIM: To investigate the hepatocytic differentiation of mesenchymal stem cells (MSCs) in co-cultures with fetal liver cells (FLC) and the possibility to expand differentiated hepatocytic cells.METHODS: MSCs were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSCs were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with stem cell factor (SCF),hepatocyte growth factor (HGF), epidermal growth factor (EGF), and fibroblast growth factor 4 (FGF-4) alone, or in presence of freshly isolated FLC. Cells in co-cultures were harvested, and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. Reverse transcription-polymerase chain reaction (RT-PCR) for the liver specific markers cytokeratin-18 (CK-18), albumin,and alpha-fetoprotein (AFP) was performed in different cell populations.RESULTS: Under the specified culture conditions, rat MSCs co-cultured with FLC expressed albumin, CK-18,and AFP-RNA over two weeks. At wk 3, MSCs lost hepatooytic gene expression, probably due to overgrowth of the cocultured FLC. FLC also showed a stable liver specific gene expression in the co-cultures and a very high growth potential.CONCLUSION: The rat MSCs from bone marrow can differentiate hepatocytic cells in the presence of FLC in vitro and the presence of MSCs in co-cultures also prorides a beneficial environment for expansion and differentiation of FLC.

  1. [Level of prolactin, gonadotrophin, testosterone, and alpha fetoprotein in serum of blood donors with antibodies to hepatitis c virus (anti-HCV)].

    Science.gov (United States)

    Rzeszowska, G; Jagiełło-Wójtowicz, E; Krawczuk, G; Baran, E; Bielec, D; Fijałka-Rymar, M; Surmaczyńska, B

    1993-01-01

    The study was aimed at the determination of serum levels of the investigated hormones (prolactin, gonadotrophins, testosterone and alpha-fetoprotein) in blood donors with antibodies to hepatitis C virus. The observed group consisted of 50 men of age between 22 and 47 years. The blood serum concentrations of hormones were determined by RIA method using the ready made reagent kits. No characteristic changes were observed in the serum hormones levels except for prolactin. Mean value of serum prolactin concentration was higher in blood donors than those in the control group. PMID:7688903

  2. The Use of Postoperative Serum HS-AFP and GGT Ⅱ for Judgment of the Prognosis for Hepatocellular Carcinoma patients

    Institute of Scientific and Technical Information of China (English)

    Runzhou Ni; Lei Yang; Mingbing Xiao; Feng Li; Cuihua Lu

    2006-01-01

    OBJECTIVE To investigate the clinical value of hepatoma-specific alpha-fetoprotein (HS-AFP) and gamma-glutamyltransferase Ⅱ (GGT Ⅱ) for judgment of postoperative prognosis of patients with hepatocellular carcinoma (HCC).METHODS HS-AFP was separated and determined using native polyacrylamide electrophoresis with a discontinuous buffer system and Western blots. GGT Ⅱ was separated with native polyacrylamide electrophoresis with a discontinuous buffer system and detected by enzyme staining. Forty cases with HCC underwent serial determination of HS-AFP and GGT Ⅱ before and after radical excision. The correlations were analyzed between the two indices and survival time.RESULTS In the 40 cases with HCC, before radical excision the positive rates of HS-AFP and GGT Ⅱ were 57.5% and 67.5% respectively, with the positive rate of combined HS-AFP and GGT Ⅱ reaching 80.0%. After operation, the recurrence and metastasis rate in the groups with positive HS-AFP and GGT Ⅱ were 90.9% and 58.8% respectively, while in the groups with negative HS-AFP and GGT Ⅱ the rates were 20.7% and 26.1% respectively. Recurrence and metastasis occurred in all cases with both postoperative positive HS-AFP and GGT Ⅱ but only in 9.5% of the cases in whom both postoperative HS-AFP and GGT Ⅱ were negative. Univariate analysis revealed that postoperative HS-AFP and GGT Ⅱ were related to the prognosis in HCC.CONCLUSION Postoperative serum HS-AFP and GGT Ⅱ are very useful in predicting the prognosis of HCC patients.

  3. A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs.

    Science.gov (United States)

    Xu, Ru; Jiang, Yandong; Xia, Lei; Zhang, Tianxiang; Xu, Lin; Zhang, Shuang; Liu, Dali; Song, Hongwei

    2015-12-15

    In this work, ZnO inverse opals structure (IOs) based photoelectrochemical (PEC) electrode was fabricated for alpha-fetoprotein (AFP) detection. Then, the uniform CdS quantum dots (QDs) were hydrothermally synthesized, which allowed the binding of AFP and glucose oxidase (GOD) on CdS QDs, forming the AFP-CdS-GOD composite. The competitive immunosensor of AFP and the AFP-CdS-GOD composite with anti-AFP antibodies (Ab) immobilized on FTO (fluorine-doped tin oxide) /ZnO IOs electrode was successfully applied to the detection of AFP. GOD could catalyze glucose to produce hydrogen peroxide (H2O2) acting as an electron donor to scavenge photogenerated holes in the valence band of CdS QDs, reducing the recombination of electrons and holes of CdS QDs. Also the effective energy level matching between the conduction bands of CdS QDs and ZnO widened the range of light absorption, allowing for electron injection from excited CdS QDs to ZnO upon visible light irradiation, which enhanced the photocurrent. The results show that the immunosensor of AFP possesses a large linear detection range of 0.1-500 ng/ml with a detection limit of 0.01 ng/ml. It also exhibits excellent anti-interference property and acceptable stability. This work provides a promising method for achieving excellent photoelectrochemical biosensor detection of other proteins. PMID:26164013

  4. A sensitive photoelectrochemical biosensor for AFP detection based on ZnO inverse opal electrodes with signal amplification of CdS-QDs.

    Science.gov (United States)

    Xu, Ru; Jiang, Yandong; Xia, Lei; Zhang, Tianxiang; Xu, Lin; Zhang, Shuang; Liu, Dali; Song, Hongwei

    2015-12-15

    In this work, ZnO inverse opals structure (IOs) based photoelectrochemical (PEC) electrode was fabricated for alpha-fetoprotein (AFP) detection. Then, the uniform CdS quantum dots (QDs) were hydrothermally synthesized, which allowed the binding of AFP and glucose oxidase (GOD) on CdS QDs, forming the AFP-CdS-GOD composite. The competitive immunosensor of AFP and the AFP-CdS-GOD composite with anti-AFP antibodies (Ab) immobilized on FTO (fluorine-doped tin oxide) /ZnO IOs electrode was successfully applied to the detection of AFP. GOD could catalyze glucose to produce hydrogen peroxide (H2O2) acting as an electron donor to scavenge photogenerated holes in the valence band of CdS QDs, reducing the recombination of electrons and holes of CdS QDs. Also the effective energy level matching between the conduction bands of CdS QDs and ZnO widened the range of light absorption, allowing for electron injection from excited CdS QDs to ZnO upon visible light irradiation, which enhanced the photocurrent. The results show that the immunosensor of AFP possesses a large linear detection range of 0.1-500 ng/ml with a detection limit of 0.01 ng/ml. It also exhibits excellent anti-interference property and acceptable stability. This work provides a promising method for achieving excellent photoelectrochemical biosensor detection of other proteins.

  5. Solitary AFP- and PIVKA-II-producing hepatoid gastric cancer with giant lymph node metastasis.

    Science.gov (United States)

    Iso, Yukihiro; Sawada, Tokihiko; Shimoda, Mitsugi; Rokkaku, Kyu; Ohkura, Yasuo; Kubota, Keiichi

    2005-01-01

    A 61-year-old man was admitted to our hospital because of abdominal pain and an abdominal mass. The patient had anemia and elevated serum alpha-fetoprotein (AFP) (9630ng/mL) and PIVKA-II (91mAU/mL) levels. Roentgenographic examination revealed an extra-gastric tumor in the upper abdomen, and gastroscopy revealed Bormann type 2 gastric cancer in the lower portion of the stomach. The preoperative diagnosis was synchronous gastric cancer and hepatocellular carcinoma (HCC), and surgery was performed. The extra-gastric tumor appeared to be an extra-hepatically growing HCC because the tumor was fed by vessels ramifying from the umbilical portion of the liver. Distal gastrectomy with resection of the extra-gastric tumor was performed, and histological examination of the resected specimen revealed that the gastric cancer was an AFP-producing hepatoid gastric adenocarcinoma and that the extra-gastric tumor was a lymph node metastasis. AFP-producing hepatoid gastric adenocarcinoma tends to metastasize to the regional lymph nodes and form a giant tumor. A giant tumor in the upper abdomen associated with gastric cancer may therefore be a clinical manifestation of AFP-producing hepatoid gastric adenocarcinoma.

  6. 采用ROC曲线评价a-L-岩藻糖苷酶,5-核苷酸酶,甲胎蛋白在肝癌诊断、预后预测中的价值%Study on using ROC curve evaluation ofα-L-fucosidase,5'-nucleo tidase and alpha fetoprotein diagnostic and predictive value in hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    刘慧敏; 刘平

    2016-01-01

    目的:探讨 a-L-岩藻糖苷酶(AFU)、5-核苷酸酶(5’-NT)、甲胎蛋白(AFP)检测对原发性肝癌(PHC)的诊断价值。方法肝癌患者(肝癌组)40例,手术后6个月,根据肝癌是否复发,分为复发组和非复发组。另入选健康体检者(对照组)60例,测定3组 AFU、5’-NT、AFP 水平,应用受试者工作曲线(ROC)评估三者对肝癌的诊断和预测价值。结果 AFU、5’-NT 和 AFP 在肝癌组和对照组间比较差异均有统计学意义。AFU、5’-NT 和 AFP 3种指标单独检测时,ROC 曲线下面积分别为0.904,0.870,0.866。手术前以及手术后复发组 AFU 浓度均高于非复发组。手术前以及手术后复发组5’-NT 浓度与非复发组相比差异无统计学意义。手术后复发组 AFP 血清浓度高于非复发组,手术前复发组 AFP 血清浓度与非复发组相比差异无统计学意义。结论通过 ROC 曲线评价,检测 AFU 和5’-NT两种指标可用于对 PHC 的诊断,AFU 可以用于肝癌预后的预测。%Objective To explore the diagnosis value of α-L-fucosidase (AFU),5'-nucleo tidase (5 ’-NT)and alpha fetoprotein (AFP)detection in patients with primary hepatic carcinoma(PHC).Methods The study involved 40 patients with hepatocellular carcinoma as experimental group and 60 cases of physical examination as control group, using ROC curve to make a comparative analysis.The patients with hepatocellular carcinoma who experienced surgical treatment were followed up for average six months.The patients were divided into relapse group and non-recurrent group,to AFU,5-NT,AFP levels were detected to assess the predictive value.Results The level of AFU,5’-NT and AFP in PHC group were higher than those in control group (P <0.05).The area under the ROC curve for the three indexes were 0.904,0.870 and 0.866,respectively.The concentrations of AFU in preoperative and postoperative detection were significantly higher than those of non-recurrent group.However,5 - NT

  7. Diagnosis value of combined detection of AFP,AFP-L3,DKK1 in primary hepatic carcinoma%AFP、AFP-L3、DKK1联合检测对原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    薄维波; 秦继宝

    2014-01-01

    Objective To analyze the clinical significance of the single detection or the combination detection of 3 kinds of tumor marker alpha-fetoprotein(AFP),alpha-fetoprotein-L3(AFP-L3)and DKK1 for the diagnosis of primary hepatic carcinoma(PHC). Methods AFP,AFP-L3 and DKK1 in the PHC group,benign hepatic diseases group and control group were detected by adopting the ECLIA,affinity adsorption chromatography and ELISA methods respectively.The ratio of AFP-L3 in AFP was calculated.Re-sults In the single detection,the sensitivity of AFP,AFP-L3 and DKK1 was 60.9%,76.8% and 69.6% respectively and the speci-ficity was 88.7%,91.5% and 95.8% respectively.In the combination detection,the sensitivity and specificity were 91.3% and 87.3% respectively,the differences between the single detection and the combination detection had statistical significance (P <0. 05).Conclusion The combined examination of serum AFP,AFP-L3 and DKK1 can elevate the sensitivity and specificity,which is much better and more accurate for diagnosing PHC than most of traditional combination detection at present.%目的:分析3种肿瘤标志物甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)、分泌型蛋白Dickkopf-1(DKK1)单独或联合检测原发性肝癌(PHC)的临床意义。方法采用电化学发光法检测 PHC 组、良性肝病组和健康对照组血清 AFP 水平;采用亲和吸附层析法检测 AFP-L3水平,计算 AFP-L3在 AFP 中比例;采用 ELISA 试剂盒检测血清标本中 DKK1水平。结果单独检测 PHC 患者的3种肿瘤标志物,AFP、AFP-L3、DKK1灵敏度分别为60.9%、76.8%、69.6%,特异度分别为88.7%、91.5%、95.8%。3项指标联合检测的灵敏度是91.3%,特异度是87.3%,与单项指标检测相比差异有统计学意义(P <0.05)。结论AFPAFP-L3、DKK1的联合检测能提高 PHC 血清学检测的灵敏度和特异度,在 PHC 诊断方面比目前大多数传统的组合方法更准确。

  8. Combined Analysis of AFP and HCCR-1 as an Useful Serological Marker for Small Hepatocellular Carcinoma: A Prospective Cohort Study

    Directory of Open Access Journals (Sweden)

    Guoxin Zhang

    2012-01-01

    Full Text Available Hepatocellular carcinoma (HCC is one of the most frequent malignant tumors in the world. The only serological marker widely used for the diagnosis of HCC is alpha-fetoprotein (AFP. Despite that AFP is widely used for the diagnosis of HCC, it has a limit as a serological marker due to its low sensitivity and specificity. The human cervical cancer proto-oncogene 1 (HCCR-1 was previously reported as a new biomarker for HCC. To further evaluate the HCCR-1 as a biomarker for HCC, we conducted the prospective cohort study. We evaluated the significance of simultaneous measurement of 2 tumor markers in the diagnosis of HCC in China, Japan and Korea. Two markers for HCC, AFP and HCCR-1, were measured in the sera obtained from 1,338 patients at the time of initial diagnosis of HCC. Of the 1338 HCC patients, 616 (46% and 686 (51.3% were sero-positive for AFP and HCCR-1, respectively. The positive rate for HCC was increased up to 74.1% in combined use of AFP and HCCR-1. Many cases (54% for AFP-negative HCC were positive for HCCR-1 and vice versa. More importantly, the diagnostic rate for small HCC (< 2 cm was significantly improved in the combined analysis of AFP and HCCR-1 to 56.9% although it was only 40.1% and 23.4% in the single analysis of HCCR-1 and AFP, respectively. Our result suggests that the HCCR-1 could be an useful biomarker for HCC while the diagnostic rate could be significantly improved in the combined use of HCCR-1 and AFP.

  9. Value of early alpha fetoprotein detection in predicting prognosis of patients with drug-induced liver injury%早期甲胎蛋白测定对药物性肝损害患者转归的预测价值

    Institute of Scientific and Technical Information of China (English)

    陈琰; 汤紫荣; 曹艳菊; 屈昌民; 梁淑文; 房慧; 胡瑾华

    2015-01-01

    Objective To investigate the clinical significance of early alpha fetoprotein (AFP) detection in predicting prognosis of patients with drug-induced liver injury (DILI).Methods Clinical data of 160 DILI patients who were admitted to 302th Hospital of Chinese People's Liberation Army from July 2011 to July 2014 were retrospectively analyzed.According to the levels of AFP within 1 week after admittion,the patients were divided into high AFP group (AFP≥20 μg/L,80 cases) and normal group (AFP <20 μg/L,80 cases).The AFP and liver function were measured.Results There were no statistical differences in sex,average age,alanine aminotransferase(ALT) and alkaline phosphatase (ALP) between high AFP group and normal group (P > 0.05).However,high AFP group showed higher level of AFP,total bilirubin and total hospitalization days compared with those in normal group[82(45,153) ng/L vs 9(3,20) ng/L,(173 ±25) μmol/L vs (70 ± 15) μmol/L,(22.3 ± 1.8) d vs (11.6 ± 1.2) d] (P < 0.01).In high AFP group the incidence of severe case was 22.5 % (18/80),statistically higher than that in normal group [1.3 % (1/80)] (P < 0.01).Conclusions High level of AFP in early stage indicates severe inflammation and poor prognosis,which may be used as an important marker for assessing the liver injury and predicting the prognosis in patients with DILI.%目的 探讨早期甲胎蛋白水平测定对药物性肝损害(DILI)转归的预测价值.方法 纳入2011年7月至2014年7月解放军第三○二医院诊断为DILI的住院患者160例,根据入院后1周内初次甲胎蛋白检测值将其分为甲胎蛋白增高组(甲胎蛋白≥20 μg/L)和甲胎蛋白正常组(甲胎蛋白<20μg/L),每组80例.对2组患者进行生存预后分析.结果 甲胎蛋白增高组和甲胎蛋白正常组年龄、性别、丙氨酸转氨酶、碱性磷酸酶比较差异无统计学意义(P>0.05);甲胎蛋白、总胆红素及住院时间比较,差异有统计学意义[82(45,153) ng/L比9

  10. The diagnostic value of des-gamma-carboxyprothrombin and alpha-fetoprotein in primary hepatocellular carcinoma%去γ-羧基凝血酶原、甲胎蛋白在原发性肝细胞癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    高明军; 陈洪; 陆枫林; 朱家沂

    2012-01-01

    目的:探讨肿瘤标志物去γ-羧基凝血酶原(des-γ-carboxy prothrombin,DCP)、甲胎蛋白(alpha-fetoprotein,AFP)联合检测对原发性肝细胞癌(hepatocellular carcinoma,HCC)的诊断价值.方法:172例研究对象分为正常对照组25例、慢性肝炎组20例、肝硬化组51例及HCC组76例,酶联免疫法(ELISA)测定血清DCP浓度,电化学发光免疫法(ECLIA)测定血清AFP浓度,应用Logistic回归、ROC曲线对DCP、AFP、两者联合检测结果进行分析评价.结果:(1)正常对照组、慢性肝炎组、肝硬化组及HCC组DCP平均浓度分别为(17.72±9.59)、(26.12±12.64)、(37.45±18.26)和(806.71±639.79) mAU/mL,DCP浓度在4组间呈递增趋势(P<0.05),且HCC组DCP浓度显著高于其他3组(P<0.01).正常对照组、慢性肝炎组、肝硬化组及HCC组AFP平均浓度分别为(7.93±5.42)、(14.59±11.91)、(16.29±14.10)和(547.47±544.98) ng/mL,HCC组AFP浓度明显高于其他3组(P<0.01).(2)DCP、AFP单独诊断HCC的ROC曲线下面积(AUROC)分别为0.891、0.813,DCP综合诊断效能优于AFP (P< 0.05);通过Logistic回归模型形成的联合预测因子(combining predictors,Comb)为分析指标,DCP、AFP联合检测时AUROC为0.932,优于DCP或AFP单独检测(P<0.05).(3)通过ROC曲线确定DCP、AFP及Comb诊断HCC的最佳界定值分别为73.35 mAU/mL、35.38 ng/mL、0.28,此时具有较高的灵敏度和特异度.结论:DCP对我国HCC患者具有较好的诊断价值,运用Logistic回归、ROC曲线及诊断界定值综合分析DCP、AFP对HCC进行诊断,大大提高了诊断效能.%Objective This study aimed to investigate the diagnostic values of the combined detection of the serum levels of des-gamma-carboxyprothrombin (DCP) and alpha-fetoprotein (AFP) in patients' with hepatocellular carcinoma. Methods Serum levels of DCP and AFP were measured in 76 patients with HCC, 51 patients with liver cirrhosis, 20 chronic hepatitis patients and 25 healthy subjects using enzyme-linked immunosorbent

  11. 不同 AFP 浓度下 AFP-L3与 GPC-3在原发性肝癌中的表达分析%Analysis of the expression of AFP-L3 and GPC-3 in primary hepatic carcinoma with different concentration of AFP

    Institute of Scientific and Technical Information of China (English)

    秦文燕; 陈博; 李自生; 佘有清

    2015-01-01

    目的:分析在不同甲胎蛋白(AFP)浓度下,甲胎蛋白异质体3(AFP-L3)、磷脂酰蛋白聚糖-3(GPC-3)在原发性肝癌(PHC)诊断中的表达,为 PHC 的诊断提供参考。方法以240例门诊、住院患者及体检者为研究对象,检测其血清 AFP、AFP-L3及 GPC-3水平;根据血清 AFP 水平分为阴性组(0≤AFP<20 ng/mL)、低浓度组(20≤AFP<400 ng/mL)和高浓度组(AFP≥400 ng/mL),比较3组 AFP-L3和 GPC-3水平,以及 AFP-L3、GPC-3单独与联合检测对 PHC 的诊断效能。结果低浓度组、高浓度组血清 AFP-L3与 GPC-3水平均高于阴性组,且高浓度组血清 AFP-L3与 GPC-3水平高于低浓度组,差异均有统计学意义(P <0.05)。AFP-L3与 GPC-3联合检测诊断 PHC 的灵敏度、特异度和准确度分别为84.4%、95.9%、93.8%,均高于 AFP-L3、GPC-3单独检测。结论联合检测 AFP-L3和 GPC-3可提高对 PHC 的诊断灵敏度、特异度和准确度,对 PHC 诊断具有重要的临床意义。%Objective To analyse the expression of alpha-fetoprotein variant-L3(AFP-L3)and glypican-3(GPC-3)in primary he-patic carcinoma(PHC)with different concentration of alpha-fetoprotein (AFP),so as to provide references for the diagnosis of PHC.Methods 240 cases of outpatients,inpatients and individuals on physical examination were selected as subjects and serum levels of AFP-L3 and GPC-3 were detected.All of the subjects were divided into negative group(0≤AFP<20 ng/mL),low concen-tration group(20≤AFP<400 ng/mL)and high concentration group(AFP≥400 ng/mL)according to the serum levels of AFP.Ser-um levels of AFP-L3 and GPC-3 were compared among the three groups.And the sensitivity,specificity and accuracy of single de-tection of AFP-L3 or GPC-3 and those of combined detection of AFP-L3 and GPC-3 were compared as well.Results The serum levels of AFP-L3 and GPC-3 in the low concentration group and high concentration group were both higher than those in the nega-tive group,and those

  12. Differentiation of human umbilical cord blood stem cells into hepatocytes in vivo and in vitro

    Institute of Scientific and Technical Information of China (English)

    Xiao-Peng Tang; Min Zhang; Xu Yang; Li-Min Chen; Yang Zeng

    2006-01-01

    AIM: To study the condition and potentiality of human umbilical cord blood stem cells (HUCBSC) to differentiate into hepatocytes in vivo or in vitro.METHODS: In a cell culture study of human umbilical cord blood stem cell (HUCBSC) differentiation, human umbilical cord blood mononuclear cells (HUCBMNC) were separated by density gradient centrifugation.Fibroblast growth factor (FGF) and hepatocyte growth factor (HGF) and the supernatant of fetal liver were added in the inducing groups. Only FGF was added in the control group. The expansion and differentiation of HUCBMNC in each group were observed. Human alpha fetoprotein (AFP) and albumin (ALB) were detected by immunohistochemistry. In the animal experiments, the survival SD rats with acute hepatic injury after carbon tetrachloride (CCL4) injection 48 h were randomly divided into three groups. The rats in group A were treated with human umbilical cord blood serum. The rats in group B were treated with HUCBMNC transplantation. The rats in group C were treated with HUCBMNC transplantation followed by intraperitoneal cyclophosphamide for 7 d.The rats were killed at different time points after the treatment and the liver tissue was histopathologically studied and human AFP and ALB detected by immunohistochemistry. The human X inactive-specific transcript gene fragment in the liver tissue was amplified by PCR to find human DNA.RESULTS: The results of cell culture showed that adherent cells were stained negative for AFP or ALB in control group. However, the adherent cells in the inducing groups stained positive for AFP or ALB. The result of animal experiment showed that no human AFP or ALB positive cells present in the liver tissue of group A (control group). However, many human AFP or ALB positive cells were scattered around sinus hepaticus and the central veins of hepatic lobules and in the portal area in group B and group C after one month. The fragment of human X chromagene could be detected in the liver tissue of

  13. Diagnosis value of AFP and AFP-L3 combined detection in hepatocellular carcinoma%血清 AFP、AFP-L3联合检测在原发性肝癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    徐恩君; 陈秋莉; 李涛; 郝丽; 肖春红; 徐元宏; 周敏; 徐胜前

    2016-01-01

    Objective To explore the diagnostic value of alpha fetoprotein (AFP), alpha fetoprotein L3(AFP-L3) and ratio of AFP-L3 to AFP in hepatocellular carcinoma(HCC).Methods In 354 patients, 110 patients with hep-atocellular carcinoma(HCC) and 244 patients with benign liver disease (BLD)(including 94 patients with hepati-tis, 126 patients with liver cirrhosis and 24 patients with benign liver tumors).AFP and AFP-L3 which was separa-ted by affinity adsorption centrifugal tube were measured by electrochemiluminescence immunoassays , following cal-culation ratio of AFP-L3 to AFP.Results In 110 patients with HCC, the patients of AFP 200 ng /ml were 32 cases, 30 cases and 48 cases respectively.In 244 patients with BLD, the patients of AFP <20 ng/ml were 196 cases.Levels of AFP, AFP-L3 and ratio of AFP-L3 to AFP in patients with HCC were significantly higher than that in patients with BLD (P <0.01).According to ROC curve, areas under the curve(AUC) of AFP, AFP-L3 and ratio of AFP-L3 to AFP were 0.809, 0.819 and 0.800 respectively, and AUC of combination three markers was 0.829(P <0.01).When the cut-off values of ratio of AFP-L3 to AFP were 5%, 10% or 15%, the sensitivities were 59.1%, 50.9% or 40.9%, and the specificities were 86.1%, 94.7%or 98.4%.Conclusion Diagnostic value of combination AFP and AFP -L3 is higher than AFP in diagnosis of hepa-tocellular carcinoma.The best cut-off value of ratio of AFP-L3 to AFP is 5%.%目的探讨甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)以及 AFP-L3比率在原发性肝癌(HCC)中的诊断价值。方法选取患者共354例,原发性肝癌(HCC)110例,良性肝病(BLD)244例(其中肝炎94例,肝硬化126例,肝良性肿瘤24例),采用亲和吸附离心管分离 AFP-L3,用电化学发光法测定 AFP 和 AFP-L3含量,计算 AFP-L3比率。结果110例 HCC 患者中,有32例 AFP <20 ng/ml,30例 AFP 20~200 ng/ml,48例 AFP >200 ng/ml;244例 BLD 患者中,196例 AFP

  14. Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.

    Science.gov (United States)

    Chou, Song; Lodish, Harvey F

    2010-04-27

    Previously we showed that the ~2% of fetal liver cells reactive with an anti-CD3epsilon monoclonal antibody support ex vivo expansion of both fetal liver and bone marrow hematopoietic stem cells (HSCs); these cells express two proteins important for HSC ex vivo expansion, IGF2, and angiopoietin-like 3. Here we show that these cells do not express any CD3 protein and are not T cells; rather, we purified these HSC-supportive stromal cells based on the surface phenotype of SCF(+)DLK(+). Competitive repopulating experiments show that SCF(+)DLK(+) cells support the maintenance of HSCs in ex vivo culture. These are the principal fetal liver cells that express not only angiopoietin-like 3 and IGF2, but also SCF and thrombopoietin, two other growth factors important for HSC expansion. They are also the principal fetal liver cells that express CXCL12, a factor required for HSC homing, and also alpha-fetoprotein (AFP), indicating that they are fetal hepatic stem or progenitor cells. Immunocytochemistry shows that >93% of the SCF(+) cells express DLK and Angptl3, and a portion of SCF(+) cells also expresses CXCL12. Thus SCF(+)DLK(+) cells are a highly homogenous population that express a complete set of factors for HSC expansion and are likely the primary stromal cells that support HSC expansion in the fetal liver.

  15. 甲胎蛋白对耐药基因MDR1表达及肝癌细胞化疗敏感性的影响%Influence of alpha-fetoprotein on the expression of drug-resistance gene MDR1 and chemotherapeutic sensitivity in hepatocellular carcinoma cells

    Institute of Scientific and Technical Information of China (English)

    吴超; 杨健; 张金玲; 金涛; 何前进; 李常海

    2015-01-01

    Objective To explore the influence of alpha-fetoprotein (AFP) on the expression of drug-resistance gene MDR1 and chemotherapeutic sensitivity in hepatocellular carcinoma (HCC) cells.Methods A HCC cell line SMMC-7721/AFP, which was stably transfected with AFP gene, was established.mRNA and protein expressions of AFP and MDR1 were detected by real-time PCR and Western Blot,respectively.The sensitivity of SMMC-7721/AFP and SMMC-7721/EGFP cells with or without MDR1 silencing by siRNA to doxorubicin was tested by MTT assay.Immunohistochemistry was used to detect the expression of MDR1 genes-coded protein Pgp in 60 cases of HCC tissues, and the relationship between Pgp expression and serum AFP levels was analyzed.Results AFP mRNA and protein could be detected in SMMC-7721/AFP cells, but not in control cells, indicating that the AFP stably transfected cell line was successfully established.MDR1 mRNA and protein levels were higher in SMMC-7721/AFP cells than those in SMMC-7721/EGFP cells.MDR1 mRNA level in SMMC-7721/AFP cells was (52.7 ± 1.5) times as high as that in SMMC-7721/EGFP cells (P < 0.05).The resistance to doxorubicin was increased by (12.8 ± 1.1) times after AFP transfection (P < 0.05).The chemosensitivity to doxorubicin was increased after the expression of MDR1 was knocked down by siRNA.The expression of Pgp in HCC tissues was positively correlated with the serum AFP levels.Conclusion AFP could induce drug-resistance to doxorubicin in HCC cells by increasing the expression of MDR1.%目的 探讨甲胎蛋白(AFP)对耐药基因MDR1表达和肝癌细胞化疗敏感性的影响.方法 建立稳定表达AFP的肝癌细胞系SMMC-7721/AFP,分别通过Real-time PCR和蛋白印迹检测转染前后AFP和MDR1的表达.MTT法测定SMMC-7721/AFP和SMMC-7721/EGFP细胞对阿霉素的化疗敏感性.siRNA沉默SMMC-7721/AFP细胞中MDR1的表达,观察细胞对阿霉素化疗敏感性的变化.采用免疫组织化学染色法检测60例肝癌组织中MDR1编码蛋

  16. Primary intracranial germ cell tumor with abnormal high value of alpha-fetoprotein after the radiation therapy. Report of case

    Energy Technology Data Exchange (ETDEWEB)

    Hokin, Kiyohiro; Abe, Hiroshi; Aida, Toshimitsu; Mabuchi, Shouji; Tsuru, Mitsuo; Nakamura, Nishio (Hokkaido Univ., Sapporo (Japan). School of Medicine)

    1983-02-01

    A 7-year-old boy was admitted to Hokkaido University Hospital complaining of headache and vomiting. On admission he was slightly confused and presented Parinaud's sign. CT scan revealed abnormal high density mass with contrast enhancement effect at the pineal region and obstructive hydrocephalus. Laboratory studies showed the normal value of human chorionic gonadotropin and no trace of alphafetoprotein. Germinoma was most suspected based on the findings of CT scan and laboratory studies. The radiation therapy was carried out for a month and CT scan taken after the radiation therapy revealed marked reduction of the size of the tumor at the pineal region, and he was discharged. But he was re-admitted 3 months after the discharge complaining of headache and vomitting again. CT scan showed the recurrence of the tumor and laboratory studies showed abnormal high value of A.F.P. After the ventriculoperitoneal shunt, the sub-occipital craniectomy was performed by the Stein's approach, and the tumor was removed. Pathologically the tumor was a typical yolk sac tumor. This case is a very interesting case because it suggests an alternation of the element of the germ cell tumor by the radiation therapy. At first admission, germinoma was the main element of the tumor judging from the effectiveness of the radiation therapy and laboratory studies. But the main element of the tumor seemed to have changed to yolk sac tumor after the radiation therapy. The relation between the tumor markers and the types of the germ cell tumor and histopathological characters of the intracranial germ cell tumor were discussed.

  17. The AFP Project

    CERN Document Server

    Staszewski, R

    2011-01-01

    AFP is a project to extend the diffractive physics programme of the ATLAS experiment by installing new detectors that will be able to tag forward protons scattered at very small angles. This will allow us to study Single Diffraction, Double Pomeron Exchange, Central Exclusive Production and photon-photon processes. This note presents the physics case for the AFP project and briefly describes the proposed detector system.

  18. Diagnosis of abnormalities of fetal nervous system by using MRI imaging and detection of serum alpha-fetoprotein%MRI联合血清甲胎蛋白检测诊断胎儿神经系统异常的研究

    Institute of Scientific and Technical Information of China (English)

    张韶琼; 金松; 凌奕

    2012-01-01

    Objective To investigate the value of MRI imaging and detection of serum alpha-fetoprotein in diagnosis of abnormalities of fetal nervous system. Methods Thirty pregnant women with normal nervous system and 30 pregnant women with abnormal nervous system by ultrasound and MRI in the Affiliated Hospital of Hainan Medical College from Juanurary 2011 o May 2012 were collected for AFP test. The pregnant women with abnormal nerous system by ultrasound were reexamined by using MRI. e results were compared. Results The levels of AFP in normal and abnormal fetus of nervous sstem were statistically differnet (P>0.05) The accurate diagbnosis rate, false positive rate and false negative rates of ultrasound and MRI in diagnosis of prenatal fetus were statistically different (x2=3.41-6.23, P< 0.05). There was no statistical difference in the rates of accurate diagnosis by MRI and MRI incombination with detection of alpha-fetoprotein.Conclusion MRI imaging technology is superior to ultrasound in diagnosis of fetus with abnormal nervous system as it can provide accuarte brain of the clinical application of fetal ultrasound is more advantages, and it can provide more accurate information of brain parenchyma and reduce misdiagnosis rate.and abortion rate%目的 显示MRI在诊断胎儿神经系统异常中的优势,及探讨MRI联合AFP在胎儿神经系统异常的价值.方法 对2011年1月~2012年5月在我院门诊及住院行产前胎儿B超及宫内MRI检查的孕妇,其中B超显示神经系统正常的有30例,B超提示神经系统异常的30例,两者均行血清AFP检查,B超提示异常的孕妇行MRI检查,对妊娠结局进行随访.比较分析B超、磁共振及磁共振联合血清甲胎蛋白检测对胎儿神经系统畸形诊断准确性.结果 (1)血清甲胎蛋白在正常胎儿与胎儿神经系统畸形的结果比较差异有统计学意义(P>0.05);(2)产前胎儿超声与核磁共振诊断准确率、假阳性率、假阴性比

  19. Construction of lentiviral vectors carrying small interference RNA of alpha-fetoprotein gene and its efficiency of target gene silencing%携带甲胎蛋白基因小干扰RNA慢病毒载体的构建及其对靶基因的沉默效率

    Institute of Scientific and Technical Information of China (English)

    程木华; 李建芳; 张峰; 曾凤伟; 谢良骏

    2012-01-01

    Objective To construct lentiviral vectors carrying small interference RNA (siRNA) of alpha-fetoprotein(AFP) followed by transfection into hepatocellular carcinoma cells and to assess the efficiency of AFP gene silencing.Methods The AFP-specific positive siRNA and non-specific negative siRNA were designed and constructed prior to insertion to the recombinant lentiviral vectors carrying green fluorescent protein (GFP) gene.This was followed by transfection into human hepatocellular carcinoma cells HepG2 and subsequent screening of cell strains with GFP expression,and were assigned into lentivirustransfected positive siRNA group and lentivirus-transfected negative siRNA group respectively.Furthermore,liposome-transfected positive siRNA group,liposome-transfected negative siRNA group,siRNA group which treated with AFP siRNA but without transfection reagent,and blank control group were established simultaneously.The efficiency of transfection was assessed under fluorescent microscope,and the relative expression of AFP mRNA and protein in HepG2 cells was determined via fluorescent quantitative polymerase chain reaction and immunoblotting assay.In addition,the inhibition rate of AFP gene expression was compared among all groups.Results Lentiviral vectors were associated with effective transfection of siRNA into HepG2 cells.The siRNA transfected with lentivirus yielded considerably higher inhibition rate of AFP mRNA than that with liposomes,as evidenced by fluorescent quantitative polymerase chain reaction (92.1%vs 74.3%,P<0.05).Immunoblotting assay showed that higher inhibition rate of AFP protein was in favor of siRNA transfected with lentivirus,but not with liposomes (88.2% vs 63.7%,P<0.05).Conclusion AFP siRNA transfected with lentivirus is associated with more effective inhibition of AFP expression in HepG2 cells.%目的 构建携带甲胎蛋白(AFP)基因小干扰RNA(siRNA)的慢病毒载体并转染肝癌细胞,评价其

  20. Diagnostic value of combined detection of soluble human leukocyte antigen-G, alpha fetoprotein and alpha fetoprotein-L3 in primary hepatocellular carcinoma%甲胎蛋白异质体联合检测对原发性肝癌的诊断价值血清可溶性人类白细胞抗原G和甲胎蛋白

    Institute of Scientific and Technical Information of China (English)

    杨收平; 李海英; 张丽

    2014-01-01

    目的:探讨可溶性人类白细胞抗原 G(HLA-G)在原发性肝癌(PHC)患者血清中的表达水平及其与甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)联合检测对 PHC 诊断价值。方法选择45例 PHC 患者、34例肝硬化患者和43例健康体检者。采用酶联免疫吸附法(ELISA)测定 HLA-G 水平,应用预装有耦联小扁豆凝集素(LCA)的琼脂糖微量离心柱分离 AFP-L3,采用化学发光法测定 AFPAFP-L3,并计算 AFP-L3/AFP 值(AFP-L3%),以 AFP>20μg/L,AFP-L3%>10%为阳性判断标准。并对3组 HLA-G、AFP、AFP-L3水平进行统计学分析。结果 PHC组、肝硬化组 HLA-G、AFP、AFP-L3水平与健康组比较差异有统计学意义(P 20 μg/L and AFP-L3% > 10% .Statistical analysis was used for HLA-G,AFP,AFP-L3 in three groups.Results There were significant significance in HLA-G,AFP and AFP-L3 levels among PHC group,liver cirrhosis group and health group (P <0.01 ),and between PHC group and liver cirrhosis group (P < 0.01 ).As for PHC diagnosis,the sensitivity of AFP was 48.9%,and the specificity 58.4%;the sensitivity of AFP-L3 66.7%,and the specificity 64.9%;the sensitivity of HLA-G was 80.0%,and the specificity 75.3%.Compared with AFP and AFP-L3,HLA-G was more sensitive and specific in the diagnosis of PHC (P <0.05).The combined detection of three markers in the diagnosis of HCC showed the sensitivity 91.1%,and specificity 84.4%.Compared the sensitivity of combined detection with single usage of HLA-G,AFP or AFP-L3,the difference was statistically significant (P <0.05).Conclusion The combined detection of HLA-G,AFP and AFP-L3 has important clinical significance for the early diagnosis and treatment of HCC.

  1. AFP-IgM复合物在肝癌中的表达与临床关系%Expression of AFP-IgM immune complexes in hepatocellular carcinoma and its clinical significance

    Institute of Scientific and Technical Information of China (English)

    蒋敬庭; 李敏; 徐斌; 郑晓; 刘检; 石亮荣; Ning Xu; Peter Nilsson-Ehle; 吴昌平; 吴骏; 秦锡虎; 孙达成; 季枚; 邓海峰; 陆明洋; 周国平

    2009-01-01

    Objective To determine the contents of the new tumor marker alpha fetoprotein immunoglobulin M immunocomplex (AFP-IgM) in hepatocellular carcinoma (HCC) and evaluate the correlation between the level of AFP-IgM and clinical feature of HCC.Methods The contents of AFP-IgM and AFP in serum of 103 healthy subjects,74 patients with HCC,43,patients with hepatic cirrhosis and 58 patients with adiposis hepatica were detected by enzyme linked immunosorbent assay and electrochemiluminescence.Results As the critical value of diagnostic significance,the best cutoff value of AFP-IgM and AFP was 300 Au/ml and 10 ug/L,respectively.Under the cutoff value,the mean serum concentrations of AFP-IgM and AFP were significantly higher in the HCC group than in the adiposis hepatica and health examination groups (P0.05),but the level of AFP-IgM was closely correlated to the tumor size and stages.Conclusion AFP-IgM is of great significance for early diagnosis of liver cancer.Meanwhile,AFP-IgM has an effect in clinical judgment of the tumor size,differentiation and so on in liver cancer.%目的 分析甲胎蛋白-IgM免疫复合物(AFP-IgM)在肝细胞癌(HCC)中的含量,探讨AFP-IgM对诊断肝癌的临床意义.方法 采用酶联免疫吸附法与电化学发光法,对103名正常人、74例肝癌、43例肝硬化和58例脂肪肝病人血清AFP-IgM与甲胎蛋白(AFP)含量检测,并分析其与临床病理特点之间的关系.结果 应用ROC曲线确定AFP-IgM和AFP的最佳切割值分别为300 Au/ml和10μg/L作为诊断学意义的临界值.在此切割值下,肝癌组AFP-IgM和AFP血清水平均高于脂肪肝组和健康体检组(P3 cm时,AFP-IgM为(604.9±749.9)Au/ml,两者比较P0.05),而与肿块大小和分期密切相关.结论 AFP-IgM对早期(Ⅰ与Ⅱ期)肝癌和小肝癌(≤3 cm)诊断具有重要作用,且对判断肝癌的肿瘤大小与分期等具有一定的临床意义.

  2. Backgrounds in AFP Detector Estimation

    CERN Document Server

    Huang, Yicong

    2016-01-01

    The ATLAS Forward Proton (AFP) detectors aim to measure protons that are scattered in the ATLAS interaction point under very small angles ($90-160 \\mu rad$). The diffractive protons detected by the AFP may be accompanied by beam halo. This report presents an estimation of the beam halo backgrounds in the AFP using low pile-up data, and position distributions of the backgrounds in the AFP.

  3. 联合检测甲胎蛋白、α-岩藻糖苷酶、β2-微球蛋白及透明质酸对诊断原发性肝癌的意义%Significance of combined detection of alpha fetoprotein, alpha fucosidase, beta 2-microglobulin and hyaluronic acid in the diagnosis of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    叶宏

    2015-01-01

    目的 探讨联合检测甲胎蛋白(AFP)、α-岩藻糖苷酶(AFU)、β2-微球蛋白(β2-m)及透明质酸(HA)对诊断原发性肝癌的意义.方法 选取2012年3月~ 2014年3月在我院治疗的原发性肝癌患者60例、其他消化系癌症患者50例、肝硬化患者50例及正常健康人60例,检测所有患者血清AFP、AFU、β2-m及HA水平.结果 原发性肝癌组患者血清AFU、AFP、β2-m、HA水平及阳性检出率明显高于其他消化系癌症组、肝硬化组及正常对照组,差异有统计学意义(P<0.05).原发性肝癌组患者血清AFU、AFP、β2-m、HA四项联合检测的敏感性、诊断符合率均优于单项检测,差异有统计学意义(P<0.05).结论 联合检测AFU、AFP、β2-m、HA四种肿瘤标志物能提高早期原发性肝癌的敏感性和诊断率,为临床早期诊治提供可靠参考.%Objective To investigate the significance of combined detection of alpha fetoprotein (AFP), alpha fucosidase (AFU), beta 2-microglobulin (β2-m) and hyaluronic acid (HA) in the diagnosis of primary liver cancer. Methods 60 patients with primary liver cancer, 50 cases of other digestive system cancer, 50 patients with hepatic cirrhosis and 60 normal people from 2012 March to 2014 March in our hospital were selected,and serum AFP, AFU, β2-m and HA levels of all patients were detected.Results The levels and positive rates of serum AFP, AFU, β2-m and HA of patients with primary liver cancer were significantly higher than those of patients with other digestive system cancer, liver cirrhosis and normal people, the difference was statiscally significant (P<0.05). The sensitivity and diagnostic accordance rate of combined detection of serum AFU, AFU, β2-m and HA in patients with primary liver cancer were better than those of the single detection, the difference was statiscally significant (P<0.05). Conclusion Combined detection of AFP, AFU, β2-m and HA four tumor markers can improve the sensitivity and diagnosis rate of

  4. Sandwich-type electrochemical immunosensor for the detection of AFP based on Pd octahedral and APTES-M-CeO₂-GS as signal labels.

    Science.gov (United States)

    Wei, Yicheng; Li, Yan; Li, Na; Zhang, Yong; Yan, Tao; Ma, Hongmin; Wei, Qin

    2016-05-15

    In the present work, an ultrasensitive sandwich-type electrochemical immunosensor based on a novel signal amplification strategy was designed for quantitative detection of alpha fetoprotein (AFP). Au nanoparticles with biocompatibility were electrodeposited on the surface of glassy carbon electrode (GCE) which can effectively capture and immobilize primary anti-AFP (Ab1) to significantly amplify the electrochemical signal. Graphene Oxide and CeO2 mesoporous nanocomposite functionalized by the 3-aminopropyltriethoxysilane supported Pd octahedral nanoparticles (Pd/APTES-M-CeO2-GS) were utilized as labels of detection anti-AFP (Ab2). Pd octahedral nanoparticles presented good catalytic activity towards the reduction of H2O2. Due to the large specific surface area and good adsorption properties of APTES-CeO2-GS nanocomposite, large amount of Pd octahedral nanoparticles could be immobilized, which could amplify the electrochemical signal and improve the sensitivity of the immunosensor. Under optimal conditions, the immunosensor exhibited wide linear range from 0.1 pg/mL to 50 ng/mL with a low detection limit of 0.033 pg/mL (S/N=3) for AFP detection. In addition, high sensitivity, excellent selectivity, good reproducibility and stability were obtained for the immunosensor, which has a promising application for quantitative detection of other tumor markers in clinical diagnosis. PMID:26745795

  5. Birth Defects Diagnosis

    Science.gov (United States)

    ... quad screen tests the levels of 4 proteins AFP (alpha-fetoprotein), hCG, estriol, and inhibin-A. Generally, ... of the proteins for which an amniocentesis tests. AFP AFP stands for alpha-fetoprotein, a protein the ...

  6. Interrelationships among biological activity, disulfide bonds, secondary structure, and metal ion binding for a chemically synthesized 34-amino-acid peptide derived from alpha-fetoprotein.

    Science.gov (United States)

    MacColl, R; Eisele, L E; Stack, R F; Hauer, C; Vakharia, D D; Benno, A; Kelly, W C; Mizejewski, G J

    2001-10-01

    A 34-amino-acid peptide has been chemically synthesized based on a sequence from human alpha-fetoprotein. The purified peptide is active in anti-growth assays when freshly prepared in pH 7.4 buffer at 0.20 g/l, but this peptide slowly becomes inactive. This functional change is proven by mass spectrometry to be triggered by the formation of an intrapeptide disulfide bond between the two cysteine residues on the peptide. Interpeptide cross-linking does not occur. The active and inactive forms of the peptide have almost identical secondary structures as shown by circular dichroism (CD). Zinc ions bind to the active peptide and completely prevents formation of the inactive form. Cobalt(II) ions also bind to the peptide, and the UV-Vis absorption spectrum of the cobalt-peptide complex shows that: (1) a near-UV sulfur-to-metal-ion charge-transfer band had a molar extinction coefficient consistent with two thiolate bonds to Co(II); (2) the lowest-energy visible d-d transition maximum at 659 nm, also, demonstrated that the two cysteine residues are ligands for the metal ion; (3) the d-d molar extinction coefficient showed that the metal ion-ligand complex was in a distorted tetrahedral symmetry. The peptide has two cysteines, and it is speculated that the other two metal ion ligands might be the two histidines. The Zn(II)- and Co(II)-peptide complexes had similar peptide conformations as indicated by their ultraviolet CD spectra, which differed very slightly from that of the free peptide. Surprisingly, the cobalt ions acted in the reverse of the zinc ions in that, instead of stabilizing anti-growth form of the peptide, they catalyzed its loss. Metal ion control of peptide function is a saliently interesting concept. Calcium ions, in the conditions studied, apparently do not bind to the peptide. Trifluoroethanol and temperature (60 degrees C) affected the secondary structure of the peptide, and the peptide was found capable of assuming various conformations in solution

  7. Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow

    Directory of Open Access Journals (Sweden)

    Vinken Mathieu

    2007-04-01

    Full Text Available Abstract Background The capability of human mesenchymal stem cells (hMSC derived of adult bone marrow to undergo in vitro hepatic differentiation was investigated. Results Exposure of hMSC to a cocktail of hepatogenic factors [(fibroblast growth factor-4 (FGF-4, hepatocyte growth factor (HGF, insulin-transferrin-sodium-selenite (ITS and dexamethasone] failed to induce hepatic differentiation. Sequential exposure to these factors (FGF-4, followed by HGF, followed by HGF+ITS+dexamethasone, however, resembling the order of secretion during liver embryogenesis, induced both glycogen-storage and cytokeratin (CK18 expression. Additional exposure of the cells to trichostatin A (TSA considerably improved endodermal differentiation, as evidenced by acquisition of an epithelial morphology, chronological expression of hepatic proteins, including hepatocyte-nuclear factor (HNF-3β, alpha-fetoprotein (AFP, CK18, albumin (ALB, HNF1α, multidrug resistance-associated protein (MRP2 and CCAAT-enhancer binding protein (C/EBPα, and functional maturation, i.e. upregulated ALB secretion, urea production and inducible cytochrome P450 (CYP-dependent activity. Conclusion hMSC are able to undergo mesenchymal-to-epithelial transition. TSA is hereby essential to promote differentiation of hMSC towards functional hepatocyte-like cells.

  8. High-performance fluorescence-encoded magnetic microbeads as microfluidic protein chip supports for AFP detection.

    Science.gov (United States)

    Gong, Xiaoqun; Yan, Huan; Yang, Jiumin; Wu, Yudong; Zhang, Jian; Yao, Yingyi; Liu, Ping; Wang, Huiquan; Hu, Zhidong; Chang, Jin

    2016-10-01

    Fluorescence-encoded magnetic microbeads (FEMMs), with the fluorescence encoding ability of quantum dots (QDs) and magnetic enrichment and separation functions of Fe3O4 nanoparticles, have been widely used for multiple biomolecular detection as microfluidic protein chip supports. However, the preparation of FEMMs with long-term fluorescent encoding and immunodetection stability is still a challenge. In this work, we designed a novel high-temperature chemical swelling strategy. The QDs and Fe3O4 nanoparticles were effectively packaged into microbeads via the thermal motion of the polymer chains and the hydrophobic interaction between the nanoparticles and microbeads. The FEMMs obtained a highly uniform fluorescent property and long-term encoding and immunodetection stability and could be quickly magnetically separated and enriched. Then, the QD-encoded magnetic microbeads were applied to alpha fetoprotein (AFP) detection via sandwich immunoreaction. The properties of the encoded microspheres were characterized using a self-designed detecting apparatus, and the target molecular concentration in the sample was also quantified. The results suggested that the high-performance FEMMs have great potential in the field of biomolecular detection. PMID:27639146

  9. High-performance fluorescence-encoded magnetic microbeads as microfluidic protein chip supports for AFP detection.

    Science.gov (United States)

    Gong, Xiaoqun; Yan, Huan; Yang, Jiumin; Wu, Yudong; Zhang, Jian; Yao, Yingyi; Liu, Ping; Wang, Huiquan; Hu, Zhidong; Chang, Jin

    2016-10-01

    Fluorescence-encoded magnetic microbeads (FEMMs), with the fluorescence encoding ability of quantum dots (QDs) and magnetic enrichment and separation functions of Fe3O4 nanoparticles, have been widely used for multiple biomolecular detection as microfluidic protein chip supports. However, the preparation of FEMMs with long-term fluorescent encoding and immunodetection stability is still a challenge. In this work, we designed a novel high-temperature chemical swelling strategy. The QDs and Fe3O4 nanoparticles were effectively packaged into microbeads via the thermal motion of the polymer chains and the hydrophobic interaction between the nanoparticles and microbeads. The FEMMs obtained a highly uniform fluorescent property and long-term encoding and immunodetection stability and could be quickly magnetically separated and enriched. Then, the QD-encoded magnetic microbeads were applied to alpha fetoprotein (AFP) detection via sandwich immunoreaction. The properties of the encoded microspheres were characterized using a self-designed detecting apparatus, and the target molecular concentration in the sample was also quantified. The results suggested that the high-performance FEMMs have great potential in the field of biomolecular detection.

  10. 甲胎蛋白与肝癌免疫的研究进展%Advanced progress in alpha-fetoprotein and hepatocarcinoma immunity

    Institute of Scientific and Technical Information of China (English)

    赵倩; 王小平; 胥冰; 蔺焕萍; 张克佩

    2016-01-01

    Hepatocarcinoma is one of the most prevalent gastroenterological cancers in the world with less effective therapy .Excepting for its diagnosis in liver cancer , AFP has become the target for liver cancer immunotherapy .Although AFP′s immunogenicity is weak and can promote the immune escapes by inhibiting the function of dendritic cells , natural killer cells and T lymphocytes , AFP has drawn more attention in liver cancer immunotherapy .By in vitro modification , the immunogenicity and immune effect of AFP could be enhanced.The important role of AFP in development , progression and immunity of liver cancer can lay a better foundation in hepato-carcinoma immunotherapy .%肝癌是常见的消化道恶性肿瘤,目前没有较好的治疗措施。甲胎蛋白在肝癌细胞中高表达,除了用于肝癌诊断外,其作为肿瘤相关抗原已成为肝癌免疫治疗的靶点。虽然甲胎蛋白免疫原性较弱,并通过抑制树突状细胞,自然杀伤细胞以及T淋巴细胞功能促进肝癌细胞免疫逃逸,但其在肝癌免疫治疗中的作用也日益受到重视,通过体外修饰增强其免疫原性促进免疫应答,从而提高杀伤肝癌细胞免疫效应。甲胎蛋白在肝癌发生、发展及免疫中的作用为研究其在肝癌中的免疫治疗奠定了良好的基础。

  11. 双标记时间分辨荧光免疫分析检测AFP和AFP-IgM在HCC中的应用%Dual-label time-resolved immunofluorometric assay of serum AFP and AFP-IgM in patients with hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    盛世乐; 王青; 黄钢; 于彬; 覃文新

    2009-01-01

    Objective Serum alpha-fetoprotein (AFP) is a widely used marker of hepatocellular carcinoma (HCC), although its diagnostic efficacy remains to be improved. Elevated levels of immunocomplex form of AFP-IgM in HCC patients have been reported. In this study a novel dual-labeled time-resolved immunofluorometric assay (dual-TRFIA) was developed to measure serum AFP and AFP-IgM levels simultaneously in order to establish a more effective serum maker detection method for HCC. Methods The dual-TRFIA for AFP-IgM or AFP was established by using Sm3+-labeled anti-human IgM monoclonal antibody (McAb) and Eu3+-labeled anti-AFP McAb as tracer antibodies. Serum AFP-IgM/AFP concentrations were measured in 118 patients with HCC, 60 patients with cirrhosis, 63 patients with chronic hepatitis and 286 healthy controls with the dual-TRFIA. The diagnostic efficacy was evaluated using receiver operating characteristic (ROC) curve. SAS 6.12 was used to analyze the data. Results The lowest detective concentrations for AFP and AFP-IgM were 0.41 ug/L and 10.00 U/L, respectively. AFP concentrations determined with duaI-TRFIA correlated well with those tested with electrochemiluminescenec immunoassay (ECLIA) (r= 0.9987, P<0.05). The sensitivity for diagnosing HCC with serum AFP and AFP-IgM was 46.61% (55/ 118) and 63.56% (75/118), respectively. The area under the ROC curve for AFP and AFP-IgM was 77.10% and 77.40%, respectively. However, the diagnostic sensitivity increased to 72.88% (86/118) (x2=9.45, P=0.02) and accuracy increased to 84.10% (x2=6.24, P=0.04) when the two markers were combined. Conclusions A highly sensitive and reliable dual-TRFIA for determination of serum AFP-IgM and AFP by a single test has been developed. It is suggested that combined measurement of serum AFP-IgM and AFP levels may improve the detection sensitivity of HCC.%目的 建立可同时测定血清甲胎蛋白(AFP)和AFP免疫复合物(AFP-IgM)的快速、灵敏检测方法,探讨其在原发性肝细胞癌(HCC

  12. Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice

    Directory of Open Access Journals (Sweden)

    Rose Chellan

    2011-04-01

    Full Text Available Abstract Background Short half-life and low levels of growth factors in the niche of injured microenvironment necessitates the exogenous and sustainable delivery of growth factors along with stem cells to augment the regeneration of injured tissues. Methods Here, recombinant human hepatocyte growth factor (HGF was incorporated into chitosan nanoparticles (CNP by ionic gelation method and studied for its morphological and physiological characteristics. Cirrhotic mice received either hematopoietic stem cells (HSC or mesenchymal stemcells (MSC with or without HGF incorporated chitosan nanoparticles (HGF-CNP and saline as control. Biochemical, histological, immunostaining and gene expression assays were carried out using serum and liver tissue samples. One way analysis of variance was used for statics application Results Serum levels of selected liver protein and enzymes were significantly increased in the combination of MSC and HGF-CNP (MSC+HGF-CNP treated group. Immunopositive staining for albumin (Alb and cytokeratin 18 (CK18, and reverse transcription-polymerase chain reaction (RT-PCR for Alb, alpha fetoprotein (AFP, CK18, cytokeratin 19 (CK19 ascertained that MSC-HGF-CNP treatment could be an effective combination to repopulate liver parenchymal cells in the liver cirrhosis. Zymogram and western blotting for matrix metalloproteinases 2 and 9 (MMP2 and MMP9 revealed that MMP2 actively involved in the fibrolysis of cirrhotic tissue. Immunostaining for alpha smooth muscle actin (αSMA and type I collagen showed decreased expression in the MSC+HGF-CNP treatment. These results indicated that HGF-CNP enhanced the differentiation of stem cells into hepatocytes and supported the reversal of fibrolysis of extracellular matrix (ECM. Conclusion Bone marrow stem cells were isolated, characterized and transplanted in mice model. Biodegradable biopolymeric nanoparticles were prepared with the pleotrophic protein molecule and it worked well for the

  13. Clinical evaluation of three tumor markers in hepatocellular carcinoma diagnosis: alpha-fetoprotein-L3, des-gamma-carboxy prothrombin and alpha-fetoprotein%甲胎蛋白异质体3、异常凝血酶原、甲胎蛋白检测肝细胞癌的对照研究

    Institute of Scientific and Technical Information of China (English)

    邝妙欢; 欧阳文婷; 林建华; 吴兴平

    2010-01-01

    目的 分析3种肿瘤标志物甲胎蛋白异质体(AFP-L3)、异常凝血酶原(DCP)、甲胎蛋白(AFP)单独或联合检测肝细胞癌(HCC)的临床意义.方法 分别用电化学发光法检测AFP,ELISA法检测AFP-L3、DCP,利用受试者工作特征曲线(ROC曲线)分析3种标志物单独和联合检测肝细胞癌(随机组和AFP阴性组)的敏感性、特异性、曲线下面积、阳性预测值和阴性预测值.比较肝细胞癌组与健康对照组和疾病对照组的AFP-L3、DCP、AFP水平的差异.分析治疗前AFP阴性肝细胞癌病例联合检测AFP-L3、DCP的意义.结果 ①单独检测肝细胞癌病例的3种肿瘤标志物.AFP-L3的敏感性、特异性分别是48.7%、97.7%;DCP的敏感性、特异性分别是42.5%、98.4%;AFP的敏感性、特异性分别是68.7%、94.6%.联合检测AFP-L3、DCP和AFP,敏感性、特异性分别是87.5%、92.2%.②肝细胞癌随机组的AFP-L3、DCP、AFP水平与健康对照组和疾病组比较差异有高度统计学意义(P<0.01).⑨AFP阴性肝细胞癌组的AFP和AFP-L3水平与肝良性疾病组比较差异无统计学意义.但DCP水平差异有统计学意义(P<0.05).结论 AFP-L3、DCP与AFP单独检测时AFP的敏感性最高,而DCP的特异性最高,是区分肝硬化与AFP阴性的肝细胞癌血清学的良好指标.AFP-L3、DCP与AFP的联合检测能提高肝细胞癌血清学检测的敏感性和特异性,在肝细胞癌诊断方面比目前大多数传统的组合方法更优胜、更准确.

  14. Expression of AFP in gastric carcinoma and its relationship with VEGF%AFP在胃癌中的表达意义及其与VEGF的关系

    Institute of Scientific and Technical Information of China (English)

    弥海宁; 赵晓宁; 杨言苹

    2011-01-01

    Objective : To investigate the clinicopathological features of alpha - fetoprotein producing gastric carcinoma and reltion with vascular endothelial growth factor.Methods : Two hundred and sixty - two cases of gastric carcinoma were divided into AFP - positive group and AFP - negative group.The expressions of AFP and VECF of all patients sample were examined by immunohistochemical method .Sex , age , tumor site , diameter , gross type , differentiation , liver and lymph node metastasis , TNM stage and prognose were compared between the two groups.Results : The rates of liver metastasis,number of metastasis lymph nodes , stage Ⅳ carcinoma in AFP - positive group were significantly higher than those in AFP - negative group( P <0.01) .The positive rate of VEGF in AFP - positive tumors was 90.5% , significantly higher than that in AFP - negative tumors 67 .2% ( P < 0.05 ) .1 ,3 ,5year - survival rates in AFP - positive group( 58.7% ,13.1% ,5.9% ) were significantly lower than those in AFP - negative group( 92.1% ,58.3% ,32.5% ) ( P <0.05) .Condusion : Alpha - fetoprotein producing gastric carcinoma has significantly higher malignant behavior and poorer prognosis, which may be related to liver and lymph node metastasis.Examining the AFP and VEGF of gastric carcinoma can be regarded as the objectivity prognostic indiciar.%目的:探讨AFPGC生物学特性及其与VEGF的关系.方法: 将262例胃癌患者分成AFP阳性和阴性两组.采用免疫组化方法检测胃癌组织中AFP及VEGF的表达,比较两组患者的性别、年龄及胃癌生长部位、大小、大体类型、分化程度、肝脏转移、淋巴结转移、临床分期以及生存率.结果:AFP阳性组肝脏转移、多个淋巴结转移发生率及临床IV期胃癌比例均明显高于AFP阴性组(P<0.01);AFP阳性组的胃癌组织中VEGF阳性表达率为90.5%,明显高于AFP阴性者的67.2% (P<0.05),AFP阳性组的1、3、5年生存率分别为58.7%、13.1%、5.9%,

  15. 甲胎蛋白与异常凝血酶原在鉴别诊断肝细胞癌中的价值%Alpha-fetoprotein and des-gamma-carboxyprothrombin in the differential diagnosis of hepatocellular carcinoma from other liver tumors

    Institute of Scientific and Technical Information of China (English)

    纪文斌; 肖年军; 罗英; 刘哲; 张宁; 孔哲; 卢实春

    2016-01-01

    目的 评估术前血清甲胎蛋白(AFP)与异常凝血酶原(DCP)在肝脏肿瘤患者中鉴别肝细胞癌的价值.方法 选取2015年1月1日至2015年5月1日解放军总医院肝胆外科收治的影像学检查证实为肝脏肿瘤的患者141例.检测患者术前血清甲胎蛋白及异常凝血酶原浓度.以病理结果或临床诊断标准(射频消融及介入栓塞治疗患者)为金标准,绘制受试者工作特征曲线并分析AFP及DCP在肝脏肿瘤患者中鉴别肝细胞癌的价值.结果 141例肝脏肿瘤患者中肝细胞癌98例,非肝细胞癌43例(包括肝内胆管细胞癌16例、肝血管瘤12例、转移性肝癌10例、局灶性结节增生3例、炎性假瘤2例).肝细胞癌组患者术前甲胎蛋白显著高于非肝细胞癌组患者[80.0(3.9~1 375.0) μg/L比2.1(1.6 ~3.2) μg/L,Z=6.98,P<0.01];肝细胞癌组患者术前异常凝血酶原显著高于非肝细胞癌组患者[141.5(24.0~978.0) AU/L比19.0(14.0 ~ 25.5) AU/L,Z=5.18,P<0.01].AFP及DCP在肝脏肿瘤患者诊断肝细胞癌的受试者工作曲线下面积(AUROC)分别为0.87(95% CI:0.81 ~0.93)及0.78(95% CI:0.69 ~0.86)(截断值分别为3.6 μg/L及35 AU/L),二者差异无统计学意义(Z=1.72,P=0.085).以20 μg/L为AFP的临界值,其诊断肝细胞癌的灵敏度为56.1%,特异度为95.4%;以40 AU/L为DCP的临界值,其灵敏度为69.4%,特异度为83.7%.在肝细胞癌患者,AFP与DCP两者检测结果相关(x2=9.12,P<0.01),相关系数r=0.292.二者联合检测(平行试验)诊断肝细胞癌的灵敏度为79.6%,特异度为81.4%.结论 DCP是肝细胞癌的有效肿瘤标志物.在肝脏肿瘤患者,DCP与AFP鉴别肝细胞癌患者能力类似;联合DCP与AFP检测有助于提高诊断灵敏度,减少漏诊.尽管肝细胞癌的诊断主要依据影像学检查,但在肿瘤影像学表现不典型时,肿瘤标志物有助于肝细胞癌的诊断.%Objective To compare the clinical utility of alpha-fetoprotein (AFP) and

  16. AFP, PIVKAII, GP3, SCCA-1 and follisatin as surveillance biomarkers for hepatocellular cancer in non-alcoholic and alcoholic fatty liver disease

    International Nuclear Information System (INIS)

    The incidence and mortality of hepatocellular cancer (HCC) complicating alcoholic and non-alcoholic fatty liver diseases (ALD and NAFLD) is rising in western societies. Despite knowing the at risk populations for HCC development, the lack of sensitive and specific means of surveillance hampers disease detection at curable stages. The most widely used serum HCC marker is alpha-fetoprotein (AFP), while PIVKA-II, glypican-3 (GP3) and Squamous Cell Carcinoma Antigen -1 (SCCA-1) have been proposed as new biomarkers. Assessment of these HCC biomarkers has largely been performed in patients with viral hepatitis. We conducted a cross sectional study assessing the value of these serum proteins, as well a novel candidate biomarker -follistatin – in patients with HCC arising on a background of ALD or NAFLD. Pre-treatment serum samples from 50 patients with HCC arising on a background of ALD (n = 31) or NAFLD (n = 19) were assessed by specific ELISA assay for PIVKAII, Glypican-3, SCCA-1 and Follistatin. Results were compared and contrasted with a control patient group with biopsy proven steatohepatitis-related cirrhosis (n = 41). The diagnostic accuracy of each of the candidate biomarkers was evaluated using receiver operating characteristic (ROC) curve analysis, reporting the area under the curve (AUC) and its 95% confidence interval (CI). Performance was compared to that of the established biomarker, AFP. Serum levels of all proteins were assessed by specific ELISA assays. GP3, SCCA-1 and follistatin had no HCC surveillance benefit in these patients. AFP and PIVKAII were superior to the other markers, particularly in combination. We conclude that while novel means of surveillance are urgently required, the combination of AFP and PIVKAII for HCC is an improvement on AFP alone in ALD/NAFLD patients. Furthermore, our data in this homogenous subset of patients- particularly that confirming no role for SCCA-1 – suggests that the choice of optimal biomarkers for HCC

  17. 32例产甲胎蛋白胃癌回顾性分析%Retrospective analysis of 32 alpha-fetoprotein-producing gastric cancer

    Institute of Scientific and Technical Information of China (English)

    田蓝天; 姚珂; 吴傲; 李龙; 张志程; 裴铁民; 刘连新

    2011-01-01

    Objectives To investigate the clinicopathologic characteristics, treatments and prognostic factors of AFPGC. Methods: 468 gastric cancer medical files preserved at the medicalrecord library were retrospectively reviewed. There were 67 patients with elevated serum level of AFP among these patients who were made a definite diagnosis of gastric cancer and subsequently received surgery and systemic combined therapy at the First Affiliated Hospital of Haerbin Medical University (FAHHMU) between January 2001 and January 2007. Having excluded some diseases as hepatocirrhosisjiver tumor, germ cell tumor which can increase the level of serum AFP, 32 (6.8%) patients were made the definite diagnosis of AFPGC. The diagnostic, therapeutic and follow up files were reviewed. Finally, these follow-up results and correlative laboratory data were taken into statistical treatment. Results: There were 32 AFPGC which accounting for 6.8% of the gastric cancer of the corresponding time period. Compared with the negative group, there was a significantly higher incidence of lymph node and liver metastasis in AFPGC group (P = 0.025 and 0.011, respectively). Clinically, AFPGC was not sensitive to the commonly used chemotherapy regimens and it couldn't suppress the progression of AFPGC. 20 of the AFPGC patients died of tumor liver metastatis and the mean postoperative servial time of the cohort was 35.7 months. Conclusion; AFPGC has more aggressive behavior than that of AFP-negative gastric cancer. Patients with AFPGC are proned to proceed liver metastatis and the prognosis is very poor. Radical correction is the preferred way to deal with AFPGC and the postoperative adjuvant chemotherapy is necessary.%目的:探讨产甲眙蛋白胃癌(α-fetoprotein-producing gastric carcinoma,AFPGC)的临床病理学特征、治疗及预后影响因素.方法:回顾分析2001年1月-2007年1月在哈尔滨医科大学附属第一医院确诊并完成治疗的468例胃癌患者住院病案

  18. Role of AFP mRNA expression in peripheral blood as a predictor for postsurgical recurrence of hepatocellular carcinoma: A systematic review and meta-analysis

    Institute of Scientific and Technical Information of China (English)

    Xiang Ding; Xiu-Shou Ling; Lian-Yue Yang; Geng-Wen Huang; Jian-Qing Yang; He-Li Liu; Wei Wang; Ji-Xiang Peng; Jie-Quan Yang; Yi-Ming Tao

    2005-01-01

    AIM: To identify the role of alpha-fetoprotein (AFP) mRNA expression in peripheral blood one week after surgery as a predictor for recurrence of hepatocellular carcinoma (HCC).METHODS: Published studies fulfilling the selection criteria were identified by searching several databases online. After a methodology assessment using a quality scale designed by European Lung Cancer Working Party, data in each research were aggregated by means of metaanalysis.RESULTS: Altogether 368 cases were included in the 9 selected studies, which fulfilled the selection criteria. The quality scores ranged from 35% to 84% with a median score of 55%. The 'design' subscore had the lowest median value (38%). By aggregating the data, a high χ2value (77.576) was presented. The fail-safe number was 136 and 64 for P = 0.05 and 0.01, respectively. CONCLUSION: AFP mRNA expression in peripheral blood 1 wk after surgery correlated with the recurrence of HCC and was a good predictor for tumor recurrence.

  19. The clinical significance of alpha-fetoprotein, human chorionic gonadotropin and unconjugated estriol in perinatal screening%甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇在产前筛查中的临床意义

    Institute of Scientific and Technical Information of China (English)

    庞春玉; 吴学礼

    2016-01-01

    目的:探讨甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇在产前筛查中的临床意义。方法:选取我院2014年12月至2015年6月孕周为15~20周的妊娠孕妇血清标本2070例,予以产前甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇的检测,并且结合孕妇的年龄、孕周、体质量等因素,运用配套的仪器产前筛查风险软件统计唐氏综合症、18–三体综合征及神经管缺陷的风险比例,予以高危孕妇做详细确诊,并依据随访与诊断结果比较高危组与低危组甲胎蛋白、人绒毛膜促性腺激素、非结合雌三醇水平,及出生缺陷阳性确诊率,产前筛查孕妇的年龄分布与出生缺陷的筛查阳性率。结果:高危组甲胎蛋白水平显著高于低危组[(28.74±1.35) vs.(6.93±1.20)μg/L],人绒毛膜促性腺激素水平显著高于低危组[(2.43±0.37) vs.(1.76±0.25) ng/L],非结合雌三醇水平明显低于低危组[(5.35±1.23) vs.(7.26±1.14)μg/L],两组比较差异明显(P35岁孕妇(26.24%);高危组孕妇出生缺陷总确诊阳性率(7.14%)明显高于低危孕妇(0.21%),两组比较差异显著。结论:甲胎蛋白、人绒毛膜促性腺激素及非结合雌三醇产前筛查能够有效筛查出生缺陷的概率,达到优生,值得推广运用。%Objective: To investigate the clinical signiifcance of alpha-fetoprotein (AFP), human chorionic gonadotropin (HCG) and unconjugated estriol (uE3) in perinatal screening.Methods: Selected 2 070 serum samples in pregnant women with gestational age of 15~20 weeks from December 2014 to June 2015 in our hospital, detected AFP, HCG and uE3 in prenatal testing, and combining with the age, gestational age, body weight and other factors, counted the riskratio of Down syndrome, trisomy 18 and the proportion of risk of neural tube defects by supporting instrument risk prenatal screening sotfware to diagnose in detail for high-risk women, and compared

  20. Alpha-fetoprotein triggers hepatoma cells escaping from immune surveillance through altering the expression of Fas/FasL and tumor necrosis factor related apoptosis-inducing ligand and its receptor of lymphocytes and liver cancer cells

    Institute of Scientific and Technical Information of China (English)

    Meng-Sen Li; Qiu-Ling Ma; Qian Chen; Xin-Hua Liu; Ping-Feng Li; Guo-Guang Du; Gang Li

    2005-01-01

    AIM: To investigate the mechanism of α-fetoprotein (AFP)in escaping from the host immune surveillance of hepatocellular carcinoma.METHODS: AFP purified from human umbilical blood was administrated into the cultured human lymphoma Jurkat T cell line or hepatoma cell line, Bel7402 in vitro. The expression of tumor necrosis factor related apoptosisinducing ligand (TRAIL) and its receptor (TRAILR) mRNA were analyzed by Northern blot and Western blot wasused to detect the expression of Fas and Fas ligand (FasL)protein.RESULTS: AFP (20 mg/L) could promote the expression of FasL and TRAIL, and inhibit the expression of Fas and TRAILR of Bel7402 cells. For Jurkat cell line, AFP could suppress the expression of FasL and TRAIL, and stimulate the expression of Fas and TRAILR. AFP also could synergize with Bel7402 cells to inhibit the expression of FasL protein and TRAIL mRNA in Jurkat cells. The monoclonal antibody against AFP (anti-AFP) could abolish these functions of AFP.CONCLUSION: AFP is able to promote the expression of FasL and TRAIL in hepatoma cells and enhance the expression of Fas and TRAILR in lymphocytes. These could elicit the escape of hepatocellular carcinoma cells from the host's lymphocytes immune surveillance.

  1. 小扁豆凝集素结合型甲胎异质体在肝癌诊断中的意义%Significance of Lens culinaris agglutinin-reactive alpha-fetoprotein in the diagnosis of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    卓传尚; 柳丽娟; 吴秋芳

    2009-01-01

    目的 探讨小扁豆凝集素结合型甲胎蛋白异质体(AFP-L3)在良恶性肝病鉴别诊断的临床价值.方法 应用装有耦联了小扁豆凝集素(LCA)的微量离心柱分离185例肝病患者的AFP-L3,用时间分辨荧光免疫检测血清AFP和AFP-L3含量,计算AFP-L3%.结果 肝细胞癌患者的AFP-L3%明显高于其他肝病患者(χ2=29.329,P<0.001);AFP、AFP-L3%检测肝细胞癌在ROC曲线下的面积AUC分别为0.407和0.841;以AFP-L3%≥12.6%作为诊断标准,AFP-L3诊断肝细胞癌敏感性和特异性分别为83.3%和86.3%.结论 AFP-L3对肝细胞癌诊断准确度明显高于AFP,微量离心柱法检测AFP-L3在良恶性肝脏病变鉴别诊断中具有重要临床价值.

  2. AFU、AFP-L3、HCY联合检测对原发性肝癌早期诊断临床价值的研究%The clinical value of the combined detection of AFU,AFP-L3 and HCY in the diagnosis of primary hepatic cancer

    Institute of Scientific and Technical Information of China (English)

    董怀平; 王力; 赵岐刚; 张延强; 李庆敏

    2007-01-01

    目的 探讨3种血清肿瘤标志物a-L岩藻糖苷酶(α-L-fucosidase,AFU)、AFP异质体(6alpha-fetoprotein variant,AFP-L3)及同型半胱氨酸(Hyperhomocysteinemia,Hcy)联合检测原发性肝癌(primary hepatic cancer,PHC)的早期诊断价值及临床界值.方法 对我院2004年11月-2005年12月收治的640例肝病患者,其中经手术和病理诊断确诊为PHC者84例,非PHC者556例.对640例肝病患者的血清联合检测AFU、AFP-L3及HCY.采用速率法测定AFU水平,电泳亲和印迹法测定AFP-L3,采用IMx同型半胱氨酸专用试剂包测定HCY.结果 PHC组血清AFU、AFP-L3及HCY水平分别为(40±18.5)U/L、(30±16.4)%、(18.9±9.7)μmol/L;非PHC患者血清AFU、AFP-L3及HCY水平分别为(16.4±9.8)U/L、(15.2±4.2)%、(9.4±4.9)μmol/L,二者相比有统计学意义(P<0.01);PHC组AFU、AFP-L3及HCY在ROC曲线下的面积(AUC)分别0.464、0.854、0.881,而三者联合检测的AUC为0.936.经ROC曲线分析,AFU40 U/L、AFP-L3%、HCY18.9μmol/L联合应用的临界值,敏感度和特异度均可达85%,阳性似然比为5.12,阴性似然比为0.17.结论 联合检测AFU、AFP-L3及HCY3项指标对HPC的早期诊断有一定的指导意义.

  3. Effects of curcumin in pediatric epithelial liver tumors: inhibition of tumor growth and alpha-fetoprotein in vitro and in vivo involving the NFkappaB- and the beta-catenin pathways

    Science.gov (United States)

    Bortel, Nicola; Armeanu-Ebinger, Sorin; Schmid, Evi; Kirchner, Bettina; Frank, Jan; Kocher, Alexa; Schiborr, Christina; Warmann, Steven; Fuchs, Jörg; Ellerkamp, Verena

    2015-01-01

    In children with hepatocellular carcinoma (pHCC) the 5-year overall survival rate is poor. Effects of cytostatic therapies such as cisplatin and doxorubicin are limited due to chemoresistance and tumor relapse. In adult HCC, several antitumor properties are described for the use of curcumin. Curcumin is one of the best-investigated phytochemicals in complementary oncology without relevant side effects. Its use is limited by low bioavailability. Little is known about the influence of curcumin on pediatric epithelial hepatic malignancies. We investigated the effects of curcumin in combination with cisplatin on two pediatric epithelial liver tumor cell lines. As mechanisms of action inhibition of NFkappaB, beta-catenin, and decrease of cyclin D were identified. Using a mouse xenograft model we could show a significant decrease of alpha-fetoprotein after combination therapy of oral micellar curcumin and cisplatin. Significant concentrations of curcuminoids were found in blood samples, organ lysates, and tumor tissue after oral micellar curcumin administration. Micellar curcumin in combination with cisplatin can be a promising strategy for treatment of pediatric HCC. PMID:26515460

  4. 微量离心柱法检测肝癌患者血清甲胎蛋白异质体临床意义分析%Application of trace column centrifugation method for the detection of serum AFP variants

    Institute of Scientific and Technical Information of China (English)

    黄海深; 周永均; 何兰香; 李宇鸣; 唐光定; 陈国强

    2014-01-01

    目的:探讨微量离心柱法检测血清甲胎蛋白(AFP)异质体(AFP-L3)在肝癌诊断及良、恶性肝病鉴别诊断中的临床应用价值。方法选择2011年6月至2012年10月于阳山县人民医院就诊的原发性肝癌(PLC)患者80例(PLC组)、肝硬化患者66例(肝硬化组)和慢性肝炎患者54例(慢性肝炎组)作为研究对象,应用国产微量离心柱法试剂分离纯化血清AFP-L3,采用化学发光法检测血清AFP和AFP-L3水平,并计算 AFP-L3在总AFP中所占比例(AFP-L3%)。结果PLC组患者血清AFP、AFP-L3%水平均高于肝硬化组和慢性肝炎组(P<0.05)。以AFP>25 ng/mL ,AFP-L3%≥10%作为阳性判断标准,PLC组患者血清AFP阳性率高于肝硬化组和慢性肝炎组,且PLC组患者血清AFP-L3%阳性率亦明显高于肝硬化组和慢性肝炎组(P<0.05),但具有不同血清AFP水平的PLC患者之间,血清AFP-L3%水平和阳性率比较差异均无统计学意义(P>0.05)。结论微量离心柱法检测血清AFP-L3%在肝癌的早期诊断和良、恶性肝病的鉴别诊断中具有重要价值。%Objective To study the clinical significance of serum alpha fetoprotein (AFP) variants (AFP-L3) detected by trace column centrifugation method for the diagnosis of liver cancer and differential diagnosis of benign and malignant liver diseases .Methods During June 2011 and October 2012 ,80 patients with primary liver cancer (PLC ,PLC group) ,66 patients with liver cirrhosis (cirrhosis group) and 54 patients with chronic hepatitis (hepatitis group) treated in this hospital were enrolled .Domestic-made trace column centrifugation kit was used to separate and purify serum AFP-L3 .AFP and AFP-L3 were detected by using chemoluminescence assay .Then ,the percentage of AFP-L3 in AFP (AFP-L3% ) was calculated .Results Levels of serum AFP and AFP-L3% in PLC group were sig-nificantly higher than cirrhosis group and hepatitis

  5. The value of combined detection of AFP, plasma prothrombin activity and serum ferritin in the prognostic of severe chronic hepatitis B%甲胎蛋白、血浆凝血酶原活动度与血清铁蛋白联合检测对慢性重型乙型肝炎预后的价值

    Institute of Scientific and Technical Information of China (English)

    李四海; 袁明娟

    2011-01-01

    Objective To analyze the joint determination of alpha - fetoprotein, plasma prothrombin activity and serum ferritin in patients with chronic severe hepatitis B for the significance prognosis.Methods Eighty chronic severe hepatitis B patients, who were divided into improved group and death group according to clinical outcomes, were observed and compared in alpha - fetoprotein (AFP), prothrombin activity (PTA) and serum ferritin (SF) with retrospective analysis.Results The AFP of the improved group at admission were higher than that of death group, and prothrombin activity and serum ferritin showed no difference between the two groups.AFP and serum ferritin showed a downward trend in the improved group while the prothrombin activity showed an upward trend; death group has been at a low level of AFP, and serum ferritin and prothrombin activity rose.Conclusion Dynamic joint detection of AFP, prothrombin activity and serum ferritin can be better used in clinical prognosis of severe hepatitis.%目的 分析慢性重型乙型肝炎患者,联合测定甲胎蛋白(AFP)、血浆凝血酶原活动度(PTA)与血清铁蛋白(SF)对于其预后的意义.方法 回顾性分析80例慢性重型乙型肝炎,并根据临床转归分为好转组、死亡组,观察并比较AFP、PTA与SF的变化情况.结果 好转组入院时AFP明显高于死亡组,而PTA和SF 2组无区别,治疗后好转组AFP、SF显著下降,而PTA显著上升;死亡组中AFP一直处于低水平,而PTA和SF显著上升.结论 动态联合检测AFP、PTA与SF临床上能更好地用于慢性乙型重症肝炎预后的判断.

  6. The Diagnosis Value of AFU and AFP Combined Detection for Primary Hepatic Carcinoma%AFU与AFP联合检测在诊断原发性肝癌中的应用

    Institute of Scientific and Technical Information of China (English)

    谢远勤

    2011-01-01

    Objective: To study the significance of serum α-L-fucosidase (AFU)and Alpha fetoprotein (AFP) in the diagnosis of primary hepatic carcinoma( PHC). Method: The activities of serum AFU and AFP were measured in 29 cases of PHC,28 eases of liver cirrhosis, 103 cases of others liver damage. Resuit: The activities in patients with PHC were significantly higher than any other groups. The diagnostic sensitivities of serum AFU and AFP in patients with PHC were 86.2% and 72.3%. AFP positive rate was 84.0% in negative of AFP for patients with PHC and the combined diagnostic sensitivity of AFU and AFU for PHC was 93.1%. Conclusion: The serum AFU and AFP is a useful marker for the diagnosis of PHC.%目的:探讨血清α-L-岩藻糖苷酶(AFU)及甲胎蛋白(AFU)对原发性肝癌(PHC)的诊断价值.方法:分别测定29例PHC患者、28例肝硬化患者、103例其他肝损伤疾病患者的AFU及AFP含量.结果:AFU与AFP在PHC中的诊断率分别为86.2%和72.3%,在AFP阴性的PHC患者中,AFU的阳性率为84%,两者联检的总阳性率为93.1%.结论:AFU与AFP联合检测对提高PHC的诊断率有重要价值.

  7. 离心管法甲胎蛋白异质体检测的临床应用探讨%Study on clinical application of lectin-reactive alpha-fetoprotein

    Institute of Scientific and Technical Information of China (English)

    徐正才; 郑晓燕; 徐春仙; 祝进

    2009-01-01

    目的:探讨离心管法分离甲胎蛋白异质体AFP-L3对鉴别肝癌、肝硬化、慢性肝炎的诊断价值.方法:采用微量离心法分离AFP-L3,然后再采用化学发光的方法检测AFP、AFP-L3,并计算AFP-3%.结果:以AFP-13%>10%作为阳性的判断标准,肝癌组敏感性为92.8%,特异性为95.0%,AFP-L3含量与血清AFP浓度及原发性肝癌病灶大小无关.结论:AFP-L3结合AFP检测对肝癌,尤其是小肝癌(400 ng/ml的限制.

  8. 甲胎蛋白异质体L3对低浓度甲胎蛋白肝癌诊断的临床意义%The diagnostic value of hepatocellular carcinoma by AFP-L3 variant determined in AFP low concentrations

    Institute of Scientific and Technical Information of China (English)

    李鲁平; 谷海峰; 陈霖; 刘爱霞; 刘佳; 徐军; 张健; 郭静霞; 赵静

    2013-01-01

    Objective To explore the clinical value of alpha-fetoprotein variant (AFP-L3) percentage in the early diagnosis of hepatocellular carcinoma (HCC) and the operation evaluation in the patients with low serum AFP level.Methods AFP-L3 variants from 245 patients (including 100 cases of cirrhosis and 145 cases of HCC) with low serum AFP level(5-40 ng/ml) were isolated and the contents of AFP-L3 variant and total serum AFP were measured to get AFP-L3% (the ratio of AFP-L3 to total AFP).The follow-up of 3 months for 100 cirrhosis patients and 12 months for 20 HCC patients who underwent operation were performed.Results Using AFP-L3% ≥ 10% as a positive criterion,there was a positive rate of 23% with AFP-L3% in 100 cases of cirrhosis,and 8 out of 23 cases of AFP-L3 positive were diagnosed to be HCC after 3 months.There was a positive rate of 46.2% with AFP-L3% in 145 cases of HCC with low serum AFP level.AFP-L3% level was significantly higher in HCC than those in cirrhosis (t =7.318,P =0.001 < 0.01).The results of AFP-L3% of 20 HCC patients who underwent operation and therapy showed that the survival rates of AFP-L3% positive cases at 12 months was 0,and the survival rates of AFP-L3% negtive cases at 12 months was 15/15.Conclusion The percentage of AFP-L3 has significant clinical values for the early diagnosis of HCC and the operation evaluation of hepatic resection in the patients with low serum AFP level.%目的 探讨在低浓度甲胎蛋白(AFP)肝病患者中,甲胎蛋白异质体L3(AFP-L3)的百分含量(AFP-L3%)对肝癌的早期诊断和疗效评估的临床意义.方法 收集245例血清低AFP含量(5 ~40 ng/ml)的肝病患者样本(其中肝硬化患者100例、肝癌患者145例),检测AFP-L3%,并对其中100例肝硬化患者和20例肝癌术后患者分别进行3个月和12个月的随访.结果 以AFP-L3%≥10%作为阳性判断标准,100例肝硬化患者中阳性为23例,经3个月随访后其中8例诊断为肝癌;145份肝癌患者血清AFP

  9. The value of the indirect immunoradiometric assay of serum alpha - fetoprotein in detecting liver regeneration and neoplastic transformation in chronic liver disease. Part of a coordinated programme on in vitro assay techniques

    International Nuclear Information System (INIS)

    To investigate the concentration of alphafetoprotein AFP in different liver diseases and above all in liver cancer the immunoradiometric assay was utilized. The results of AFP studies were compared with regeneration index, blastic T lymphocytes transformation as well as other morphological and biochemical data. The results of the investigations indicated that: 38% of chronic benign hepatopathies displayed the values of serum AFP in normal ranges, 54% were in the range of 41 - 200ng/ml, and 8% showed 200 and more ng/ml. The most important conclusion from the work performed was that the elevation of serum AFP level in the evaluation of chronic hepatopathies, especially in cirrhoses, appears as an index of malignancy

  10. Clinical Value of Combined Measurement of Serum Alpha- Fetoprotein, Alpha- L- Fucosidase and Ferritin Levels in the Diagnosis of Primary Liver Cancer%AFP、AFU、SF联检对原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    张爱民; 柴小红; 靳颖; 董雪梅

    2005-01-01

    目的:探讨血清甲胎蛋白(AFP)、α-L-岩藻糖苷酶(AFU)和铁蛋白(SF)联检对提高原发性肝癌阳性检出率的意义.方法:检测52例原发性肝癌患者和40例正常对照组血清AFP、AFU、SF的含量.结果:AFP、AFU、SF三项指标对原发性肝癌患者的阳性率分别为82.7%、86.5%、76.9%.AFP+AFU、AFP+SF、AFP+AFU+SF联检阳性率分别为94.2%、90.4%、98.1%.结论:血清AFP、AFU、SF联检可提高对原发性肝癌的诊断阳性率.

  11. 血清AFP、CEA、AFU、GGT-Ⅱ联合检测诊断原发性肝癌的临床价值%Clinical value of combined measurement of serum alpha-fetoprotein, carcinoembryonic antigen, alpha-L-fucosidase, gamma-glutamyltransferase isoenzymes for diagnosis of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    宁珠; 殷芳; 刘海; 尤丽英; 杨晋辉; 郑盛

    2013-01-01

    Objective To evaluate the clinical value of combined measurement of four serum tumor markers (alpha-fe-toprotein, carcinoembryonic antigen, alpha-L-fucosidase, gamma-glutamyltransferase isoenzymes) for diagnosis of primary liver cancer. Methods 160 patients with primary liver cancer and 120 healthy subjects were measured the serum levels of alpha-fetoprotein, carcinoembryonic antigen, alpha-L-fucosidase, gamma-glutamyltransferase isoenzymes was detected by using not continuous PAGE. The results were analyzed by SPSS 15.0 software. Results The serum levels of alpha-fetoprotein, carcinoembryonic antigen, alpha-L-fucosidase were significantly higher in the primary liver cancer patients than those in the control group (P < 0.01). The positive rate of single marker of alpha-fetoprotein, carcinoembryonic antigen, alpha-L-fucosidase, gamma-glutamyltransferase isoenzymes was 68.00%, 28.00%, 84.00% and 77.00% respectively, and there were statistical significances during carcinoembryonic antigen, alpha-L-fucosidase, gamma-glutamyltransferase isoenzymes (all P < 0.05). The positive rate of the combined measured of two markers: alpha-fe-toprotein/carcinoembryonic antigen, alpha-fetoprotein/alpha-L-fucosidase and alpha-fetoprotein/gamma-glutamyltrans-ferase isoenzymes were 75.00%, 84.00% and 89.00% respectively, and there were statistical significances during the positive rates of the combined measured of alpha-fetoprotein/alpha-L-fucosidase, alpha-fetoprotein/gamma-glutamyl-transferase isoenzymes compared with alpha-fetoprotein (all P < 0.05). But the positive rate of combined measurement of four markers was 96.00% and there was evidently statistical significance compared with the alpha-fetoprotein result (P < 0.01). Conclusion The combined measurement of alpha-fetoprotein, carcinoembryonic antigen, alpha-L-fucosidase, gamma-glutamyltransferase isoenzymes can be used as an accessory tool in diagnosis of primary liver cancer.%目的 探讨血清甲胎蛋白、癌胚抗原、

  12. Rat bone marrow mesenchymal stem cells differentiate into hepatocytes in vitro

    Institute of Scientific and Technical Information of China (English)

    Xin-Qin Kang; Wei-Jin Zang; Tu-Sheng Song; Xiao-Li Xu; Xiao-Jiang Yu; Dong-Ling Li; Ke-Wei Meng; Sheng-Li Wu; Zhi-Ying Zhao

    2005-01-01

    AIM: To investigate the mechanism and regulation of differentiation from bone marrow mesenchymal stem cells (MSCs) into hepatocytes and to find a new source of celltypes for therapies of hepatic diseases. METHODS: MSCs were isolated by combining gradient density centrifugation with plastic adherence. The cells were cultured in osteogenic or adipogenic differentiation medium and determined by histochemical staining. MSCs were plated in plastic culture flasks that were not coated with components of extracellular matrix (ECM). When MSCs reached 70% confluence, they were cultured in low glucose Dulbecco's modified Eagle's medium supplemented with 10 mL/L fetal bovine serum, 20 ng/mL hepatocyte growth factor (HGF) and 10 ng/mL fibroblast growth factor-4 (FGF-4). The medium was changed every 3 d and stored for albumin, alpha-fetoprotein (AFP) and urea assay. Glycogen store of hepatocytes was determined by periodic acid-Schiff staining.RESULTS: By combining gradient density centrifugation with plastic adherence, we isolated a homogeneous population of cells from rat bone marrow and differentiated them into osteocytes and adipocytes. When MSCs were cultured withFGF-4 and HGF, approximately 56.6% of cells became smallround and epithelioid on d 24 by morphology. Compared with the control, levels of AFP increased significantly from d 12 to 15.5±1.4 μg/L (t = 2.31, P<0.05) in MSCs cultured with FGF-4and HGF, and were higher (46.2±1.5 μg/L)ond 21 (t = 41.926, P<0.01), then decreased to 24.8±2.2 μg/L on d 24 (t = 10.345, P<0.01). Albumin increased significantly on d 21 (t= 3.325, P<0.01) to 1.4±0.2 μg/mL,and to 2.1±0.7 μg/mL on d 24 (t= 3.646, P<0.01). Urea(2.3±0.4 mmol/L) was first detected on d 21 (t = 6.739, P<0.01), and continued to increase to 2.6±0.9 mmol/Lon d 24 (t= 4.753, P<0.01). Glycogen storage was first seen on d 21.CONCLUSION: The method combining gradient density centrifugation with plastic adherence can isolate MSCs. Rat MSCs may be

  13. 血清CA125、甲胎蛋白及胎盘生长因子在胎盘早剥预测中的初步研究%A preliminary study of serum CA125, alpha - fetoprotein, and placental growth factor for prediction of placental abruption

    Institute of Scientific and Technical Information of China (English)

    刘世凯; 宋莉莉; 陈铎

    2012-01-01

    目的:检测胎盘早剥、重度子痫前期及正常妊娠孕妇的血清CA125、甲胎蛋白和胎盘生长因子水平,探讨其与胎盘早剥的关系,为胎盘早剥的预测及早期诊断提供理论依据.方法:采用微粒酶免疫分析法( MEIA)和双抗体夹心ELISA法检测孕妇血清CA125、甲胎蛋白和胎盘生长因子水平.结果:胎盘早剥组CA125和甲胎蛋白明显高于其他两组,胎盘生长因子明显低于其他两组(P<0.05).结论:检测外周血CA125、甲胎蛋白和胎盘生长因子水平对于预测胎盘早剥具有一定的临床价值.%Objective: To detect the levels of serum CA125, alpha-fetoprotein, and placental growth factor in the pregnant women with placental abruption, the pregnant women with severe preeclampsia, and the normal pregnant women, explore their relationships with placental abruption, provide a theoretical basis for prediction and early diagnosis of placental abruption. Methods: Micropartical enzyme immu-noassay and double - antibody sandwich ELISA were used to detect the levels of serum CA125, alpha - fetoprotein, and placental growth factor. Resnlts: The levels of serum CA123 and alpha - fetoprotein in placental abruption group were significantly higher than those in the other two groups, the level of placental growth factor in placental abruption group was significantly lower than those in the other two groups (P <0.05) . Conclusion: Detecting the levels of CA125, alpha - fetoprotein, and placental growth factor in peripheral blood has a certain clinical value for predicting placental abruption.

  14. AFP, PIVKAII, GP3, SCCA-1 and follisatin as surveillance biomarkers for hepatocellular cancer in non-alcoholic and alcoholic fatty liver disease

    Directory of Open Access Journals (Sweden)

    Trerotoli Paolo

    2008-07-01

    Full Text Available Abstract Background The incidence and mortality of hepatocellular cancer (HCC complicating alcoholic and non-alcoholic fatty liver diseases (ALD and NAFLD is rising in western societies. Despite knowing the at risk populations for HCC development, the lack of sensitive and specific means of surveillance hampers disease detection at curable stages. The most widely used serum HCC marker is alpha-fetoprotein (AFP, while PIVKA-II, glypican-3 (GP3 and Squamous Cell Carcinoma Antigen -1 (SCCA-1 have been proposed as new biomarkers. Assessment of these HCC biomarkers has largely been performed in patients with viral hepatitis. We conducted a cross sectional study assessing the value of these serum proteins, as well a novel candidate biomarker -follistatin – in patients with HCC arising on a background of ALD or NAFLD. Methods Pre-treatment serum samples from 50 patients with HCC arising on a background of ALD (n = 31 or NAFLD (n = 19 were assessed by specific ELISA assay for PIVKAII, Glypican-3, SCCA-1 and Follistatin. Results were compared and contrasted with a control patient group with biopsy proven steatohepatitis-related cirrhosis (n = 41. The diagnostic accuracy of each of the candidate biomarkers was evaluated using receiver operating characteristic (ROC curve analysis, reporting the area under the curve (AUC and its 95% confidence interval (CI. Performance was compared to that of the established biomarker, AFP. Results Serum levels of all proteins were assessed by specific ELISA assays. GP3, SCCA-1 and follistatin had no HCC surveillance benefit in these patients. AFP and PIVKAII were superior to the other markers, particularly in combination. Conclusion We conclude that while novel means of surveillance are urgently required, the combination of AFP and PIVKAII for HCC is an improvement on AFP alone in ALD/NAFLD patients. Furthermore, our data in this homogenous subset of patients- particularly that confirming no role for SCCA-1 – suggests

  15. Immunotherapy of hepatocellular carcinoma with dendritic cells vaccine targeting to alpha-fetoprotein%以AFP为靶点的肝癌树突状细胞免疫治疗的实验研究

    Institute of Scientific and Technical Information of China (English)

    张利旺; 张红梅; 贾军; 任军

    2005-01-01

    目的探讨AFP作为肝癌基因治疗和免疫治疗靶点的可行性以及AFP基因转染的树突状细胞(AFP-DC)疫苗对表达AFP肝细胞癌的免疫治疗作用.方法构建AFP-cDNA 真核表达载体,体外转染DC,制备AFP-DC瘤苗,诱导针对表达AFP的肝癌细胞株HepG2的特异性免疫反应,MTT法检测效应细胞对靶细胞的杀伤率.结果 AFP-DC瘤苗能够分泌AFP抗原,培养上清中AFP含量为0.8805 IU/ml,与空载体组和空白对照组相比,差异显著(P<0.05).免疫荧光检测可见AFP-DC胞浆及胞膜有AFP抗原分子表达;活化的CTL能够对表达AFP肝癌细胞起特异性杀伤作用,杀伤效率可达84.05%,与空载体组和空白对照组比较,差异显著(P<0.05).结论 AFP作为靶点治疗肝癌具有可行性,AFP可作为肝癌靶向治疗的新的突破点;AFP-DC疫苗可以作为表达AFP肝癌的一种免疫治疗手段,为DC瘤苗的临床应用奠定了基础.

  16. The tracking system of the AFP detector.

    CERN Document Server

    Dyndal, M; The ATLAS collaboration

    2014-01-01

    AFP is a project to extend the diffractive physics programme of the ATLAS experiment by installing new detectors that will be able to tag forward protons scattered at a very small angles. In this talk I will present the status of AFP silicon tracker development: sensor qualification, actual design of mechanics and Geant4 simulation results with integrated ATLAS forward region description.

  17. 慢性乙型肝炎免疫清除期甲胎蛋白与HBV DNA清除的相关性研究%Correlation between serum alpha-fetoprotein level and HBV DNA clearance in immune clearance phase of patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    庞晓鹰; 徐洪涛; 杨秀珍; 咸建春; 沈美龙

    2014-01-01

    目的:探讨乙型肝炎免疫清除期,甲胎蛋白(AFP)与HBV DNA清除之间的相关性。方法收集处于免疫清除期发病2周内AFP升高5倍以上患者共58例,分为非抗病毒治疗组(下称观察组)31例和核苷(酸)类似物抗病毒治疗组(下称阳性对照组)27例,另取同期住院AFP阴性首次发病的慢性乙型肝炎核苷(酸)类似物抗病毒患者30例作为阴性对照组。分析影响HBV DNA清除率的相关因素。结果阳性对照组及阴性对照组患者均行抗病毒治疗,HBV DNA定量与各临床数据之间均无相关性,观察组HBV DNA定量与各指标之间的关系结果显示,AFP与HBV DNA清除具有显著相关性(r=0.8420,P=0.018),以下依次为ALT(r=0.7888,P=0.027)和总胆红素(TBil)(r=0.7816,P=0.032)。HBsAg(r=0.0480,P=0.413)和HBeAg(r=0.3356,P=0.191)与HBV DNA清除无显著相关性。结论对乙型肝炎免疫清除期AFP升高5倍以上患者,可先进行密切病情观察,根据病情需要进行抗病毒治疗。%Objectives To investigate the relationship between serum alpha-fetoprotein level and HBV DNA clearance in immune clearance phase of patients with chronic hepatitis B (CHB). Methods Total of 58 cases with CHB, who were in immune clearance phase and the serum alpha-fetoprotein level>5 upper limits of normal in two weeks since disease onset were enrolled. Among which 31 cases received non-antiviral therapy as experimental group, and 27 cases received antiviral therapy as positive control group. While other 30 cases with CHB who were in immune clearance phase and the serum alpha-fetoprotein level were normal in two weeks since disease onset, were enrolled as negative control group. And the correlation factors of HBV DNA clearance were analyzed, respectively. Results There were no signiifcant correlation between HBV DNA clearance and other data in patients who received antiviral therapy in positive and negative

  18. The diagnostic value of alpha-fetoprotein, α-L-fucosidase,gamma-glutamyltransferase, alkaline phosphatase and carbohydrate antigen 19-9 in the diagnosis of primary hepatocellular carcinoma%甲胎蛋白、α-L-岩藻糖苷酶、γ-谷氨酰转移酶、碱性磷酸酶及糖类抗原19-9联合检测在原发性肝癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    潘倩雄

    2014-01-01

    Objective To investigate the diagnostic value of alpha-fetoprotein (AFP),α-L-fucosidase(AFU),gamma-glutamyltransferase(GGT),alkaline phosphatase (ALP) and carbohydrate antigen 19-9(CA19-9) in the diagnosis of primary hepatocellular carcinoma(PHC).Methods Seventy-eight PHC patients as PHC group,76 patients with other liver diseases as other liver diseases group and 60 healthy volunteers as control group.The level of serum AFP,AFU,GGT,ALP,CA19-9 and positive rate in each group were compared.Results The level of AFP,AFU,GGT,ALP,CA19-9 in PHC group were (276.3 ±72.6)μg/L,(63.1 ± 11.7) U/L,(268.5 ±57.2) U/L,(293.4 ±61.7) U/L and (56.9 ± 12.5) μg/L,in other liver diseases group were (81.6 ± 12.1) μg/L,(28.3 ±7.3) U/L,(54.9 ±7.8) U/L,(116.5 ± 23.8) U/L and (27.8 7.1) μ g/L,in control group were (13.8 ± 2.7) μ g/L,(12.6 ± 3.9) U/L,(12.3 ± 3.2) U/L,(47.2 ± 11.3) U/L and (12.9 ± 3.4) μ g/L.The level of above index in PHC group and other liver diseases group were significantly higher than those in control group,while the level of above index in PHC group were significantly higher than those in other liver diseases group (P < 0.01 or < 0.05).The positive rate of AFP,AFU,GGT,ALP,CA19-9 in PHC group were 62.8%(49/78),79.5%(62/78),83.3% (65/78),85.9% (67/78),66.7% (52/78),and significantly higher than those in other liver diseases group[31.6%(24/76),32.9%(25/76),27.6%(21/76),22.4%(17/76),15.8 %(12/76)].The positive rate of AFP in control group was 0,the positive rate of AFU,GGT,ALP,CA19-9 in control group were 1.7% (1/60),3.3% (2/60),1.7% (1/60),8.3% (5/60),which significantly lower than those in other two groups.The positive rate of combined detection in PHC group was 100.0%(78/78),which significantly higher than that in other liver diseases group [40.8%(31/76)] and control group[8.3%(5/60)](P< 0.01).Conclusion The combined detection of AFP,AFU,GGT,ALP,CA19-9 in PHC diagnosis has a higher sensitivity,and thus to make

  19. Automated Ply Inspection (API) for AFP Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The Automated Ply Inspection (API) system autonomously inspects layups created by high speed automated fiber placement (AFP) machines. API comprises a high accuracy...

  20. Retrospective study of the diagnostic value of quantitative alpha-fetoprotein in infants with terato-mas%婴儿畸胎瘤甲胎蛋白量化检测的临床意义探讨

    Institute of Scientific and Technical Information of China (English)

    何小庆; 王珊; 刘苗; 阮味维

    2014-01-01

    -fetopro-tein (AFP)in infants compared with the upper limit of normal reference values in mature,immature and malig-nant teratomas. Methods The clinical data of 1 66 infants with teratomas underwent in this institute from Oc-tober 1 991 to May 201 0 were retrospectively analyzed. The value of serum AFP was defined as the ratios of chemiluminescent immunoassay measurement of AFP compared with upper limit of normal reference values.Analyze different value of serum AFP change rules in differential diagnostic the mature,immature and malig-nant teratomas with relation to the pathological diagnosis. Results ①The maximum value of serum AFP was 1 6.34(absolute value 670 ng/mL)in 1 31 cases of mature teratomas. The serum AFP value was significant difference in immature teratomas. The minimum serum AFP value was 1.25 and the maximum was 39.27 in 7 of 1 3 immature teratomas grade Ⅰ,while the minimum AFP value was 1.23 and the maximum was 1 39.51 in 5 of 1 3 immature teratomas gradeⅡ,2 of 1 3 were more than 83.1 7. Whose AFP value was higher than the up-per limit of the detection ranges was gradeⅢin 1 of 1 3 immature teratomas. The minimum serum AFP value in 22 was 83.1 7(absolute value 499 ng/mL)and higher than the upper limit of the detection ranges in 1 3 of 22 malignant teratomas;②The positive rate of serum AFP in immature teratomas(1 2,92.31%)and malignant teratomas(22,1 00%)was significantly higher than mature teratomas(58,44.28%)(P0.05 ). Compared to the less than 9-month-old infants(33,84.62%),the positive rate of serum AFP was higher than the older than 9 months of age (59,46.46%)(P <0.05 ).The positive rate of serum AFP in gonadal teratomas (20, 76.92%)higher than extragonadal teratoma(72,51.43%)(P<0.05). Conclusion The positive rate of AFP was affected by age,tumor localization,histopathologic and other factors. The value of serum AFP differs in different histopathologic teratomas. Our results indicated that the variation rules of serum AFP value have im

  1. Hepatic Differentiation of Human Induced Pluripotent Stem Cells in a Perfused Three-Dimensional Multicompartment Bioreactor

    Science.gov (United States)

    Freyer, Nora; Knöspel, Fanny; Strahl, Nadja; Amini, Leila; Schrade, Petra; Bachmann, Sebastian; Damm, Georg; Seehofer, Daniel; Jacobs, Frank; Monshouwer, Mario; Zeilinger, Katrin

    2016-01-01

    Abstract The hepatic differentiation of human induced pluripotent stem cells (hiPSC) holds great potential for application in regenerative medicine, pharmacological drug screening, and toxicity testing. However, full maturation of hiPSC into functional hepatocytes has not yet been achieved. In this study, we investigated the potential of a dynamic three-dimensional (3D) hollow fiber membrane bioreactor technology to improve the hepatic differentiation of hiPSC in comparison to static two-dimensional (2D) cultures. A total of 100 × 106 hiPSC were seeded into each 3D bioreactor (n = 3). Differentiation into definitive endoderm (DE) was induced by adding activin A, Wnt3a, and sodium butyrate to the culture medium. For further maturation, hepatocyte growth factor and oncostatin M were added. The same differentiation protocol was applied to hiPSC maintained in 2D cultures. Secretion of alpha-fetoprotein (AFP), a marker for DE, was significantly (p < 0.05) higher in 2D cultures, while secretion of albumin, a typical characteristic for mature hepatocytes, was higher after hepatic differentiation of hiPSC in 3D bioreactors. Functional analysis of multiple cytochrome P450 (CYP) isoenzymes showed activity of CYP1A2, CYP2B6, and CYP3A4 in both groups, although at a lower level compared to primary human hepatocytes (PHH). CYP2B6 activities were significantly (p < 0.05) higher in 3D bioreactors compared with 2D cultures, which is in line with results from gene expression. Immunofluorescence staining showed that the majority of cells was positive for albumin, cytokeratin 18 (CK18), and hepatocyte nuclear factor 4-alpha (HNF4A) at the end of the differentiation process. In addition, cytokeratin 19 (CK19) staining revealed the formation of bile duct-like structures in 3D bioreactors similar to native liver tissue. The results indicate a better maturation of hiPSC in the 3D bioreactor system compared to 2D cultures and emphasize the potential of dynamic 3D culture

  2. The T-box transcription factor Brachyury regulates epithelial–mesenchymal transition in association with cancer stem-like cells in adenoid cystic carcinoma cells

    International Nuclear Information System (INIS)

    The high frequencies of recurrence and distant metastasis of adenoid cystic carcinoma (AdCC) emphasize the need to better understand the biological factors associated with these outcomes. To analyze the mechanisms of AdCC metastasis, we established the green fluorescence protein (GFP)-transfected subline ACCS-GFP from the AdCC parental cell line and the metastatic ACCS-M GFP line from an in vivo metastasis model. Using these cell lines, we investigated the involvement of the epithelial–mesenchymal transition (EMT) and cancer stem cell (CSCs) in AdCC metastasis by real-time RT-PCR for EMT related genes and stem cell markers. Characteristics of CSCs were also analyzed by sphere-forming ability and tumorigenicity. Short hairpin RNA (shRNA) silencing of target gene was also performed. ACCS-M GFP demonstrated characteristics of EMT and additionally displayed sphere-forming ability and high expression of EMT-related genes (Snail, Twist1, Twist2, Slug, zinc finger E-box binding homeobox 1 and 2 [Zeb1 and Zeb2], glycogen synthase kinase 3 beta [Gsk3β and transforming growth factor beta 2 [Tgf-β2]), stem cell markers (Nodal, Lefty, Oct-4, Pax6, Rex1, and Nanog), and differentiation markers (sex determining region Y [Sox2], Brachyury, and alpha fetoprotein [Afp]). These observations suggest that ACCS-M GFP shows the characteristics of CSCs and CSCs may be involved in the EMT of AdCC. Surprisingly, shRNA silencing of the T-box transcription factor Brachyury (also a differentiation marker) resulted in downregulation of the EMT and stem cell markers. In addition, sphere-forming ability, EMT characteristics, and tumorigenicity were simultaneously lost. Brachyury expression in clinical samples of AdCC was extremely high and closely related to EMT. This finding suggests that regulation of EMT by Brachyury in clinical AdCC may parallel that observed in vitro in this study. The use of a single cell line is a limitation of this study. However, parallel data from in vitro and

  3. 南昌地区妊娠14~20周妇女血清甲胎蛋白和游离绒毛膜促性腺激素的MOM值测定及临床应用%Detection and clinical application of MOM values of serum alpha-fetoprotein and free human chorionic gonadotropin in pregnant women during 14~20 gestational weeks in Nanchang region

    Institute of Scientific and Technical Information of China (English)

    赖华; 刘艳秋; 刘淮

    2011-01-01

    目的:运用计算出的AFP及F-βHCG的MOM值与目前采用的MOM值对孕妇进行胎儿神经管缺陷畸形及唐氏综合征筛查,期待能更有效地筛查出南昌地区胎儿神经管缺陷及唐氏综合征高危孕妇.方法:依据2005~2007年到江西省妇幼保健院产前诊断门诊就诊的孕妇各孕周AFP、F-βHCG的中位数,计算出AFP、F-βHCG的MOM值,用计算出的MOM值(研究组)和目前采用的MOM值(对照组)分别对2008年来该院产前诊断门诊就诊的孕妇进行胎儿神经管缺陷畸形及唐氏综合征筛查,对筛查出的高危孕妇进行羊水检测和随访,比较两种MOM值对胎儿神经管缺陷畸形和唐氏综合征筛查的阳性率、假阴性率及假阳性率.结果:神经管缺陷畸形、唐氏综合征阳性率研究组高于对照组,具有统计学意义(P<0.01);假阴性率研究组与对照组比较,结果无统计学意义;假阳性率研究组较对照组降低,结果具有统计学意义,(神经管缺陷畸形P<0.05,唐氏综合征P<0.01).结论:应用本地区MOM值进行神经管缺陷畸形及唐氏综合征筛查,能更有效地筛查出本地区的神经管缺陷畸形及唐氏综合征胎儿.%Objective: To screen fetal neural tube defect and Down's syndrome by calculated MOM values and values currently used of serum alpha - fetoprotein (AFP) and free β human chorionic gonadotropin (F - β HCG) in pregnant women, in order to screen out the high risk pregnant women of fetal neural tube defect and Down's syndrome in Nanchang region.Methods: The MOM values of AFP and F - β HCG were calculated according to the medians of AFP and F - β HCG in pregnant women of different gostational weeks who visited the prenatal diagnosis outpatient of the hospital from 2005 to 2007, then the calculated MOM values ( study group) and the MOM values currently used ( control group) were used to screen fetal neural tube defect and Down's syndrome among the pregnant women who visited the prenatal

  4. Construction and Identification of Expression Vector of siRNA Specific for Alpha-Fetoprotein%甲胎蛋白特异性小干扰RNA表达载体的构建及鉴定

    Institute of Scientific and Technical Information of China (English)

    郏雁飞; 胡安拉; 汪运山; 周芳; 马晓丽; 苏占涛

    2007-01-01

    目的:构建特异性抑制甲胎蛋白(AFP)的小干扰RNA(siRNA)表达载体,为进一步研究AFP基因功能及AFP相关肿瘤的基因治疗奠定基础.方法:设计并合成AFP特异性的短链寡核苷酸,退火形成双链DNA片段,通过与RNAi-ReadypSIREN-DNR-DsRed-Express Donor Vector连接、转化大肠杆菌、扩增、纯化得到所需质粒,用琼脂糖凝胶电泳及基因测序鉴定其分子量及插入片段的序列.结果:琼脂糖凝胶电泳证实纯化后的质粒大小约为6 740 bp,测序证实插入序列与合成的寡核苷酸序列完全符合.结论:构建了AFP特异性的siRNA表达质粒pSIREN-DNR-DsRed-Express Donor Vector-AFP.

  5. Stages of Adult Primary Liver Cancer

    Science.gov (United States)

    ... tumor markers . An increased level of alpha-fetoprotein (AFP) in the blood may be a sign of ... conditions, including cirrhosis and hepatitis, may also increase AFP levels. Sometimes the AFP level is normal even ...

  6. Tests for Liver Cancer

    Science.gov (United States)

    ... has come back after treatment Alpha-fetoprotein blood (AFP) test AFP is normally present at high levels in the ... liver disease, liver cancer, or other cancers. If AFP levels are very high in someone with a ...

  7. Treatment Option Overview (Adult Primary Liver Cancer)

    Science.gov (United States)

    ... tumor markers . An increased level of alpha-fetoprotein (AFP) in the blood may be a sign of ... conditions, including cirrhosis and hepatitis, may also increase AFP levels. Sometimes the AFP level is normal even ...

  8. Treatment Options for Adult Primary Liver Cancer

    Science.gov (United States)

    ... tumor markers . An increased level of alpha-fetoprotein (AFP) in the blood may be a sign of ... conditions, including cirrhosis and hepatitis, may also increase AFP levels. Sometimes the AFP level is normal even ...

  9. General Information about Adult Primary Liver Cancer

    Science.gov (United States)

    ... tumor markers . An increased level of alpha-fetoprotein (AFP) in the blood may be a sign of ... conditions, including cirrhosis and hepatitis, may also increase AFP levels. Sometimes the AFP level is normal even ...

  10. The Correlation between Serum Levels of CA125 and AFP and Immature Teratoma of the Ovary%CA125和AFP与卵巢未成熟畸胎瘤的相关性研究

    Institute of Scientific and Technical Information of China (English)

    张素仙; 刘惠谨; 廖红云; 胡万芹; 杨晓玲

    2012-01-01

    Objective To disscuss the correlation between immature teratoma of the ovary and serum CA125 or AFP. Methods The serum levels of CA125 and AFP were measured in 102 patients with simple ovarian teratoma. The patients were divided into 32 cases of ovarian immature teratoma and 70 cases of ovarian mature teratoma according to surgery and pathology results. The serum levels of CA125 and AFP were compared between two groups. The 30 cases of immature teratoma were followed up, and the roles of CA125 and AFP in the recurrence were compared. Results The average levels and sensitivity of CA125 and AFP in patients with immature teratoma were higher than in patients with mature teratoma, the difference was statistically significant (P < 0.01). The decrease of AFP levels in the recurrence of cases of ovarian immature teratoma was not obvious. Conclusions CA125 and AFP have some reference value in in the differential diagnosis of immature and mature ovarian teratoma. AFP may be indicators of the early recurrence and prognosis of ovarian immature teratoma.%目的 探讨血清糖链蛋白125(carbohydrate antigen 125,CA125)、甲胎蛋白(alpha-fetoprotein,AFP)与卵巢未成熟畸胎瘤(immature teratoma of the ovary,IT)的相关性.方法 测定102例单纯型卵巢畸胎瘤的血清CA125、血清AFP,并按手术后病理结果分为IT 32例,卵巢成熟畸胎瘤(mature cys tic teratoma of the ovary,MCT)70例,分析CA125、AFP检测水平在卵巢成熟及未成熟畸胎瘤诊断中的差异,并对30例未成熟畸胎瘤进行随访,评价CA125,AFP在复发病例中的作用.结果 IT的CA125、AFP平均水平及敏感性均高于MCT,差异有统计学意义(P<0.01).AFP在卵巢未成熟畸胎瘤术后复发病例中下降不明显.结论 CA125、AFP联合检测对卵巢未成熟型与成熟型畸胎瘤的鉴别诊断有一定参考价值;AFP可能是评价卵巢未成熟畸胎瘤早期复发及判断预后的指标.

  11. The construction and expression of AFP-enhanced green fluorescent protein recombinant and the analysis of measurement characteristics%甲胎蛋白-增强型绿色荧光蛋白重组体的构建表达及其测量特性分析

    Institute of Scientific and Technical Information of China (English)

    张健; 葛丹红; 王雪亮

    2014-01-01

    Objective To construct the fusion protein of alpha fetoprotein (AFP )-enhanced green fluorescent protein(eGFP)recombinant,and to analyze the stability and measurement characteristics.Methods The sequence of eGFP was amplified from pcDNA3.0 plasmid and inserted PET28a plasmid to construct expression plasmid PET28a-eGFP.The coding sequence of AFP was synthetized according to the coding sequence of AFP from the National Center for Biotechnology Information (NCBI)database,added and linked to eGFP sequence by a linker sequence to construct expression plasmid PET28a-eGFP-AFP.Recombinant protein eGFP and eGFP-AFP were purified,and the measurement characteristics and stability were analyzed.Results The expression plasmid of recombinant protein eGFP and eGFP-AFP were constructed,and the proteins were purified.Recombinant protein eGFP and eGFP-AFP had the same excitation spectrum and emission spectrum,which were 450 and 509 nm optimally,and its fluorescence could be stable over 1 2 months.eGFP-AFP could be tested for the level of AFP by routine AFP immunoassay.Conclusions Recombinant protein eGFP and eGFP-AFP have the same fluorescence characteristics and could react with routine immunoassay which establishes the foundation for the next research using eGFP-AFP as calibration and quality control materials.%目的:构建增强型绿色荧光蛋白(eGFP)标记的甲胎蛋白(AFP)的融合蛋白,并对其稳定性和测量特性进行分析。方法从pcDNA3.0质粒中扩增eGFP序列,插入质粒PET28a,构建eGFP表达质粒PET28a-eGFP。根据美国国立生物技术信息中心(NCBI)数据库中的AFP编码序列,应用序列合成方法合成AFP编码序列,并加入连接eGFP的铰链序列,与eGFP序列链接,构建表达质粒PET28a-eGFP-AFP。分别表达和纯化重组蛋白eGFP和eGFP-AFP,分析重组蛋白的荧光特性及稳定性。结果成功构建和纯化重组蛋白eGFP和eGFP-AFP,荧光光谱分析显示其激发和发射光

  12. 肝切除を施行した肝細胞癌患者におけるalpha-fetoprotein(AFP)とprotein induced by vitamin K deficiency or antagonist-II(PIVKA-II)の重要性における検討

    OpenAIRE

    山元, 謙太郎

    2010-01-01

    報告番号: 甲26042 ; 学位授与年月日: 2010-03-24 ; 学位の種別: 課程博士 ; 学位の種類: 博士(医学) ; 学位記番号: 博医第3521号 ; 研究科・専攻: 医学系研究科外科学専攻

  13. Fetal ductus venosus flow velocity waveforms and maternal serum AFP before and after first-trimester transabdominal chorionic villus sampling

    NARCIS (Netherlands)

    C.A. Brezinka (Christoph); A.M. Hagenaars (A.); J.W. Wladimiroff (Juriy); F.J. Los

    1995-01-01

    textabstractDoppler flow velocity waveform recording in the fetal ductus venosus and umbilical artery as well as maternal blood sampling for serum alpha-fetoprotein (MSAFP) was performed before and after transabdominal chorion villus sampling (TACVS) in 36 women of advanced maternal age (≥ 36 years)

  14. Construction and expression of recombined human AFP eukaryotic expression vector

    Institute of Scientific and Technical Information of China (English)

    Li-Wang Zhang; Yang-Lin Pan; Stephen M Festein; Jun Ren; Liang Zhang; Hong-Mei Zhang; Bin Jin; Bo-Rong Pan; Xiao-Ming Si; Yan-Jun Zhang; Zhong-Hua Wang

    2003-01-01

    AIM: To construct a recombined human AFP eukaryotic expression vector for the purpose of gene therapy and target therapy of hepatocellular carcinoma (HCC).METHODS: The full length AFP-cDNA of prokaryotic vector was digested, and subcloned to the multi-clony sites of the eukaryotic vector. The constructed vector was confirmed by enzymes digestion and electrophoresis, and the product expressed was detected by electrochemiluminescence and immunofluorescence methods.RESULTS: The full length AFP-cDNA successfully cloned to the eukaryotic vector through electrophoresis, 0.9723 IU/ml AFP antigen was detected in the supernatant of AFPCHO by electrochemiluminescence method. Compared with the control groups, the differences were significant (P<0.05).AFP antigen molecule was observed in the plasma of AFPCHO by immunofluorescence staining.CONCLUSION: The recombined human AFP eukaryotic expression vector can express in CHO cell line. It provides experimental data for gene therapy and target therapy of hepatocellular carcinoma.

  15. 21 CFR 866.6030 - AFP-L3% immunological test system.

    Science.gov (United States)

    2010-04-01

    ... 21 Food and Drugs 8 2010-04-01 2010-04-01 false AFP-L3% immunological test system. 866.6030... Systems § 866.6030 AFP-L3% immunological test system. (a) Identification. An AFP-L3% immunological test... measure, by immunochemical techniques, AFP and AFP-L3 subfraction in human serum. The device is...

  16. 干细胞移植治疗失代偿期肝硬化患者110例临床评估%Clinical analysis of stem cell transplantation on treatment of 110 patients with decompensated cirrhosis

    Institute of Scientific and Technical Information of China (English)

    黄丽雯; 刘黎; 周健

    2011-01-01

    目的 观察干细胞移植治疗失代偿期肝硬化的疗效.方法 110例失代偿期肝硬化患者在常规治疗基础上行干细胞移植,移植后第4周复查肝功能、凝血酶原时间(PT)、胆碱酯酶(CHE)和AFP等实验室指标及观察临床症状和体征的变化.结果 移植后第4周,血浆白蛋白(ALB)和CHE分别为(31.2±4.5) g/L和(80.9±22.7)U/L,较移植前显著升高(t=2.95,P<0.01;t=2.06,P<0.05),PT为(22.9±3.6)s,较治疗前显著下降(t=2.18,P< 0.05),但ALT、AST、总胆红素(TBil)和AFP水平在移植前后无显著性变化(t=1.32、1.58、1.05、1.57,P> 0.05);干细胞移植可明显改善失代偿期肝硬化患者临床症状和体征,提高生存质最,术中未发生严重并发症,近期无不良反应出现.结论 干细胞移植治疗失代偿期肝硬化有较好的近期疗效.%Objective To observe the clinic effect of stem cell transplantation on treatment of patients with decompensated cirrhosis.Methods 110 patients with decompensated cirrhosis were treated by stem cell transplantation based on conventional treatment.The liver function,prothrombin time (PT),cholinesterase and alpha-fetoprotein (AFP)were detected after 4 weeks,and the changes of symptoms and signs were observed.Results Four weeks after transplantation,the levels of plasma albumin (ALB) and cholinesterase were (31.2 ± 4.5) g/L and (80.9± 22.7) U/L respectively,which were increased significantly ( t =2.95,P < 0.01 ; t =2.06,P < 0.05).The levels of PT was (22.9 ± 3.6)s,which decreased significantly ( t =2.18,P < 0.05),but the levels of aspartate aminotransferase (ALT),alanine aminotransferase (AST),total bilirubin (TBil) and AFP had no significant changes ( t =1.32,1.58,1.05,1.57,P > 0.05)before and after the transplantation.The clinical symptoms,signs and life quality of patients with decompensated cirrhosis could be significantly improved by stem cell transplantation.There were no adverse reactions and serious intraoperative

  17. Stages of Childhood Liver Cancer

    Science.gov (United States)

    ... β-hCG) or a protein called alpha-fetoprotein (AFP). Other cancers and certain noncancer conditions, including cirrhosis and hepatitis , can also increase AFP levels. Complete blood count (CBC) : A procedure in ...

  18. Risks of Liver (Hepatocellular) Cancer Screening

    Science.gov (United States)

    ... cancer is present in the body. Alpha-fetoprotein (AFP) is the most widely used tumor marker for ... and other types of cancer, may also increase AFP levels. Specific tumor markers that may lead to ...

  19. How Is Testicular Cancer Diagnosed?

    Science.gov (United States)

    ... proteins called tumor markers , such as alpha-fetoprotein (AFP) and human chorionic gonadotropin (HCG). When these tumor ... that there is a testicular tumor. Rises in AFP or HCG can also help doctors tell which ...

  20. Spina Bifida (For Parents)

    Science.gov (United States)

    ... by taking certain prenatal tests . The alpha-fetoprotein (AFP) test is a blood test done between the ... weeks of pregnancy. This test measures how much AFP, which the fetus produces, has passed in the ...

  1. General Information about Testicular Cancer

    Science.gov (United States)

    ... are used to detect testicular cancer: Alpha-fetoprotein (AFP). Beta-human chorionic gonadotropin (β-hCG). Tumor marker ... places in the body, and blood levels of AFP, β-hCG, and LDH). Type of cancer. Size ...

  2. Liver (Hepatocellular) Cancer Screening

    Science.gov (United States)

    ... cancer is present in the body. Alpha-fetoprotein (AFP) is the most widely used tumor marker for ... and other types of cancer, may also increase AFP levels. Specific tumor markers that may lead to ...

  3. General Information about Childhood Liver Cancer

    Science.gov (United States)

    ... β-hCG) or a protein called alpha-fetoprotein (AFP). Other cancers and certain noncancer conditions, including cirrhosis and hepatitis , can also increase AFP levels. Complete blood count (CBC) : A procedure in ...

  4. In situ hybridization of neuropeptide-encoding transcripts afp-1, afp-3, and afp-4 in neurons of the nematode Ascaris suum.

    Science.gov (United States)

    Nanda, Jennifer Cho; Stretton, Antony O W

    2010-03-15

    The gene transcripts encoding both the AF8 and AF2 neuropeptides of the nematode Ascaris suum have been identified, cloned, and sequenced. The AF8 transcript (afp-3) encodes five identical copies of AF8; each peptide-encoding region is flanked by the appropriate dibasic or monobasic cleavage processing sites. The AF2 transcript (afp-4) encodes three identical copies of AF2 along with the appropriate cleavage sites. In contrast, the afp-1 transcript (Edison et al. [1997] Peptides 18:929-935) encodes six different AF peptides (AF3, 4, 10, 13, 14, 20) which all share a -PGVLRFamide C-terminus but have different N-terminal sequences. By using in situ hybridization, gene transcript expression patterns of afp-1, afp-3, and afp-4 (As-flp-18, As-flp-6, and As-flp-14, respectively, in the naming convention proposed by Blaxter et al. [1997] Parasitol Today 13:416-417) were determined in the adult A. suum anterior nervous system. Each gene transcript can be localized to a different subset of neurons. These subsets of neurons are different from the subsets of Caenorhabditis elegans neurons that were shown to express identical or similar peptides by the use of promoter GFP constructs (Kim and Li [2004] J Comp Neurol 475:540-550). PMID:20058230

  5. 血清异常凝血酶原和甲胎蛋白联合检测对原发性肝癌的临床价值%The clinical value of serum PIVKA-Ⅱ and AFP detection for hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    席强; 孙桂荣; 丛培珊; 刘明军; 宗金宝

    2014-01-01

    Objective To discuss the clinical value of Protein induced by Vitamin K Antagonist-Ⅱ (PIVKA-Ⅱ) and alpha-Fetoproteins (AFP) in diagnosing hepatocellular carcinoma (HCC) and monitoring the treatment effects.Methods Patients were recruited by the Affiliated Hospital of Qingdao University,from August 2013 to March 2014.Serum levels of PIVKA-Ⅱ and AFP were measured by both chemiluminescence assay (CLIA) and electrochemiluminescence assay (ECLA) in patients with HCC (n =148),intrahepatic cholangiocellular carcinoma (n =37),gastric cancer and colorectal cancer (n =44),cirrhosis (n =63),chronic hepatitis B (n =38) and healthy subjects (n =57).To analyze the areas under the receiver operating characteristic curves (ROC-AUC) and to compare the sensitivity and specificity of single PIVKA-Ⅱ or AFP assay,and the combined detection.To analyze the correlation of PIVKA-Ⅱ and both tumor size and TNM staging,so do AFP,respectively.To compare the serum level changes of the two indicators in HCC patients before and after treatment.Results The serum levels of both PIVKA-Ⅱ and AFP in HCC group were higher than that in intrahepatic cholangiocellular carcinoma,gastric cancer and colorectal cancer,cirrhosis,chronic hepatitis B and healthy subjects groups (PIVKA-Ⅱ:U =866.50,424.00,958.00,292.00 and 448.00 ; AFP:U=713.00,440.50,1 182.00,614.00 and 399.00,P <0.001).The ROC-AUCs of the single PIVKA-Ⅱ or AFP assay and the combined detection in HCC group were not statistically different (P > 0.05).The sensitivity of PIVKA-Ⅱ (87.16%) was higher than that of AFP (68.92%,x2 =4.73,P < 0.05) in diagnosing HCC ; the sensitivity of the combined detection of PIVKA-Ⅱ and AFP(93.24%) was higher than that of PIVKA-Ⅱ itself (87.16%,adjusted x2 =64.70,P < 0.01) ;while the specificities among them did not show statistical significance (P > 0.05).Tested by Spearman rank correlation,the serum levels of PIVKA-Ⅱ and AFP were both positively related to tumor size (r =0

  6. Status of the ATLAS Forward Physics (AFP) project

    Science.gov (United States)

    Chytka, Ladislav; Atlas Collaboration

    2013-04-01

    The ATLAS Forward Physics (AFP) project plans to add a set of detectors - silicon 3D pixel tracking detectors and QUARTIC time of flight detectors - in the forward region of the ATLAS experiment at the LHC. The AFP detectors will be placed around 210 m from the interaction point and are meant to detect protons produced at small angles. The detectors are to be housed in the so called Hamburg beam pipe - a movable beam pipe allowing horizontal movement of the detectors. The AFP is currently under approval with possible installation in 2014/15.

  7. Status of the ATLAS Forward Physics (AFP) Project

    CERN Document Server

    Chytka, L; The ATLAS collaboration

    2012-01-01

    The ATLAS Forward Physics (AFP) project plans to add a set of detectors --- silicon 3D pixel tracking detectors and QUARTIC time of flight detectors --- in the forward region of the ATLAS experiment at the LHC. The AFP detectors will be placed around 210 m from the interaction point and are meant to detect protons produced at small angles. The detectors are to be housed in the so called Hamburg beam pipe --- a movable beam pipe allowing horizontal movement of the detectors. The AFP is currently under approval with possible installation in 2014/15.

  8. Study on the role of asialoglycoprotein receptor for human bone marrow mesenchymal stem cells against hepatitis B virus infection%骨髓间充质干细胞对乙型肝炎病毒的易感性及与无涎糖蛋白受体的关系

    Institute of Scientific and Technical Information of China (English)

    谢婵; 谢仕斌; 张绍全; 谢俊强; 林炳亮; 高志良

    2010-01-01

    Objective To investigate the susceptibility of bone marrow mesenchymal stem cell (BMSC) to hepatitis B virus (HBV) infection during induction toward hepatocyte and the role of asialoglycoprotein receptor (ASGPR) in BMSC HBV infection. Methods BMSC obtained from hepatitis B patients were tested for HBV infection and then cultured with HBV infectious serum in vitro and induced to differentiate into hepatocyte through exposure to hepatocyte growth factor (HGF), fibroblast growth factor-4(FGF-4), and epidermal growth factor(EGF). Subsequently these cells were determined for the presence of hepatitis B virus e antigen( HBeAg), hepatitis B virus surface antigen(HBsAg) and ASGPR. All experiments were repeated for 3 times in 5 different samples. The results were analyzed by non-parametric test. Results After 6 days of exposure, BMSC-derived hepatocyte-like cells expressed hepatic special genes and proteins, including alpha fetoprotein(AFP),cytokeratin18 (CK18), albumin (Alb), and manifested hepatocyte functions, including glycogen synthesis, urea secretion and albumin synthesis. Expressions of CK18 and Alb were increased, and AFP was decreased with time of induction. The BMSC were resistant to HBV infection both in vitro and in vivo or after induction toward hepatocyte. ASGPR expression level was low in BMSC, which was increased in the induced BMSC but still lower than that of the control HepG2 cells. Conclusions BMSC are resistant to HBV infection both in vitro and in vivo. The low level expression of ASGPR may be a reason for this.%目的 评价骨髓间充质干细胞(BMSC)向肝细胞诱导过程中对HBV的易感性及无涎糖蛋白受体(ASGPR)对BMSC感染HBV的作用.方法 体外使用肝细胞生长因子、成纤维细胞生长因子-4和表皮生长因子,将BMSC诱导分化为肝细胞.检测乙型肝炎患者BMSC的HBV感染情况,并对原代及诱导培养后的BMSC进行体外HBV感染实验,检测BMSC感染后的HBsAg、HBcAg表达情况,并检测BMSC

  9. Isolation, culture and identification of adult hepatic stem cells in vitro%成体肝脏干细胞的体外分离、培养及鉴定

    Institute of Scientific and Technical Information of China (English)

    吕国悦; 张平; 李红; 李爽; 王广义

    2007-01-01

    trypsinase was added, and subsequently, anti-human C-kit antibody, immunomagnetic beads and Buffer solution were added in order. C-kit+ cells were preliminarily isolated by immunomagnetic bead separation. Haematoxylin-eosin staining and immunofluorescent histochemical double-staining were used for detecting the hepatic stem cells in para-cancerous tissues.MAIN OUTCOME MEASURES: ① Observation of cell morphology. ② Identification of hepatic stem cells from para-cancerous tissues. ③ Identification of C-kit+ cells by immunofluorescent histochemical double-staining.RESULTS :① After primarily cultured for 2 weeks, the adherent cells grew in colony. After one half of culture medium was renewed, mature hepatocytes were gradually broken and disappeared. Small round cells propagated, and most of them were located in the center and arranged in cluster. Most cells were found with one big nucleus in each, less cytoplasm and clear cell boundary. When cells propagated to the 1st and 2nd generations, they still grew in colony, but fast. Each C-kit+ cell isolated by immunomagnetic bead separation presented a spherical cell body with a very big nucleus and less cytoplasm. After in vitro cultured for 1 week, it presented broken pieces and apoptotic symptoms.② After para-cancerous tissue was stained by haematoxylin-eosin, atypically proliferated biliary tracts with small round cells could be seen in the portal area. After para-cancerous tissue was stained by immunofluorescent histochemical double-staining, small round cells in the biliary tracts proliferated in the portal arsa co-expressed red fluorescence AFP and green fluorescence cytokeratin (CK) 19 with yellow superposition arsa. ③ After C-kit+ cells were stained by fluorescence immunocytochemisty, cytoplasm expressed alpha-fetoprotein (AFP) red granules and CK19 green granules. The superposition area of both presented yellow fluorescence of AFP+/CK19+-positive cells.CONCLUSION: Hepatic stem cells exist in human para

  10. Feto portador de síndrome de turner e tetralogia de fallot associadas à elevação de alfafetoproteína materna Fetal turner syndrome and tetralogy of fallot associated with elevated maternal serum alpha-fetoprotein levels

    Directory of Open Access Journals (Sweden)

    Eduardo Vieira Neto

    1998-06-01

    Full Text Available A síndrome de Turner fetal e suas complicações, a hidropisia e o higroma cístico, podem produzir alteração dos marcadores bioquímicos de soro materno inicialmente utilizados no rastreamento de síndrome de Down e de defeitos de tubo neural (DTN. Os autores relatam o caso de uma gestante de 37 anos, que foi rastreada para síndrome de Down e DTN no início do 2º trimestre. Foi constatado aumento da alfafetoproteína de soro materno (MSAFP e o rastreamento foi considerado positivo para DTN. Foi realizado exame ultra-sonográfico tridimensional, que não demonstrou nenhuma anormalidade fetal ou placentária, caracterizando o caso como elevação idiopática de MSAFP. No 3º trimestre, a gravidez evoluiu com acentuada oligoidrâmnia e alteração do fluxo uteroplacentário, obrigando à instituição de terapia com corticosteróides e parto cesáreo na 34ª semana gestacional. O concepto do sexo feminino foi encaminhado à UTI neonatal, onde foram diagnosticadas tetralogia de Fallot e síndrome de Turner. Esse caso incentivou os autores a rever a literatura sobre marcadores bioquímicos de soro materno na síndrome de Turner e nas malformações cardíacas congênitas. Ao final, propõe-se um protocolo para elevação idiopática de MSAFP.Turner syndrome and its complications, hydrops and cystic hygroma, can produce alterations in maternal serum biochemical markers used in screening for Down's syndrome and neural tube defects (NTD. The authors report the case of a 37-year-old pregnant woman, screened for Down's syndrome and NTD in the second trimester of pregnancy. The maternal serum alpha-fetoprotein (MSAFP level was increased and the test was considered screen positive for NTD. A three-dimensional ultrasound investigation was performed, but no fetal or placental anomalies were found, indicating a case of unexplained increased msafp. In the third trimester severe oligohydramnios and disturbances in uteroplacental arterial circulation

  11. The alpha-fetoprotein enhancer region activates the albumin and alpha-fetoprotein promoters during liver development

    OpenAIRE

    Jin, Lin; Long, Lingyun; Green, Michael A.; Spear, Brett T.

    2009-01-01

    The four members of the albumin gene family encode the serum transport proteins albumin, α-fetoprotein, α-albumin, and vitamin D-binding protein. These genes are transcribed primarily in the liver with each having a different pattern of developmental expression. The tight linkage of these genes, particularly that of albumin, α-fetoprotein and α-albumin, and their liver-specific expression, has led to the suggestion that these genes share common regulatory elements. To directly examine whether...

  12. Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling

    LENUS (Irish Health Repository)

    Abdel Aziz, Mohamed T

    2011-05-05

    Abstract Background The present study was conducted to evaluate the tumor suppressive effects of bone marrow derived mesenchymal stem cells (MSCs) in an experimental hepatocellular carcinoma (HCC) model in rats and to investigate the possible role of Wnt signaling in hepato-carcinogenesis. Methods Ninety rats were included in the study and were divided equally into: Control group, rats which received MSCs only, rats which received MSCs vehicle only, HCC group induced by diethylnitroseamine (DENA) and CCl 4 , rats which received MSCs after HCC induction, rats which received MSCs before HCC induction. Histopathological examination and gene expression of Wnt signaling target genes by real time, reverse transcription-polymerase chain reaction (RT-PCR) in rat liver tissue, in addition to serum levels of ALT, AST and alpha fetoprotein were performed in all groups. Results Histopathological examination of liver tissue from animals which received DENA-CCl4 only, revealed the presence of anaplastic carcinoma cells and macro-regenerative nodules type II with foci of large and small cell dysplasia. Administration of MSCs into rats after induction of experimental HCC improved the histopathological picture which showed minimal liver cell damage, reversible changes, areas of cell drop out filled with stem cells. Gene expression in rat liver tissue demonstrated that MSCs downregulated β-catenin, proliferating cell nuclear antigen (PCNA), cyclin D and survivin genes expression in liver tissues after HCC induction. Amelioration of the liver status after administration of MSCs has been inferred by the significant decrease of ALT, AST and Alpha fetoprotein serum levels. Administration of MSCs before HCC induction did not show any tumor suppressive or protective effect. Conclusions Administration of MSCs in chemically induced HCC has tumor suppressive effects as evidenced by down regulation of Wnt signaling target genes concerned with antiapoptosis, mitogenesis, cell proliferation

  13. Status of the AFP project in the ATLAS experiment

    CERN Document Server

    Tasevsky, Marek; The ATLAS collaboration

    2015-01-01

    Status of the AFP project in the ATLAS experiment is summarized. The AFP system is composed of a tracker to detect intact, diffractively scattered protons, and of a time-of-flight detector serving to suppress background from pile-up interactions. The whole system, located around 210~m from the main ATLAS detector, is placed in Roman Pots which move detectors from and to the incident proton beams. A typical distance of the closest approach of the tracker to these beams is 2--3~mm. The main physics motivation lies in measuring diffractive processes in runs with not a very high amount of pile-up.

  14. Status of the AFP project in the ATLAS experiment

    Energy Technology Data Exchange (ETDEWEB)

    Taševský, Marek [Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21 Prague (Czech Republic)

    2015-04-10

    Status of the AFP project in the ATLAS experiment is summarized. The AFP system is composed of a tracker to detect intact, diffractively scattered protons, and of a time-of-flight detector serving to suppress background from pile-up interactions. The whole system, located around 210 m from the main ATLAS detector, is placed in Roman Pots which move detectors from and to the incident proton beams. A typical distance of the closest approach of the tracker to these beams is 2–3 mm. The main physics motivation lies in measuring diffractive processes in runs with not a very high amount of pile-up.

  15. Status of the AFP project in the ATLAS experiment

    Science.gov (United States)

    Taševský, Marek

    2015-04-01

    Status of the AFP project in the ATLAS experiment is summarized. The AFP system is composed of a tracker to detect intact, diffractively scattered protons, and of a time-of-flight detector serving to suppress background from pile-up interactions. The whole system, located around 210 m from the main ATLAS detector, is placed in Roman Pots which move detectors from and to the incident proton beams. A typical distance of the closest approach of the tracker to these beams is 2-3 mm. The main physics motivation lies in measuring diffractive processes in runs with not a very high amount of pile-up.

  16. AFP与AFU和CAI9-9联合检测对原发性肝癌的诊断意义%Value of combined measurement of AFP,AFU and CAI9-9 in diagnosing primary hepatic carcinoma

    Institute of Scientific and Technical Information of China (English)

    许可银; 张弘; 朱郁飞

    2011-01-01

    目的:探讨肿瘤标志物甲胎蛋白(alpha-fetoprotein,AFP)、α-L-岩藻糖苷酶(alpha-L-fucosidase,AFU)、糖类抗原(CAI9-9)联合检测在原发性肝癌(primary hepatocellular carcinoma,PHC)诊断中的应用价值.方法:应用电化学发光免疫分析法和全自动生化分析仪法测定70例PHC患者和50例正常对照组血清AFP、AFU、CA19-9的含量,并分析对PHC诊断的敏感性和特异性.结果:PHC组AFP、APU、CA19-9水平明显高于正常对照组,差异极有统计学意义(P<0.01);AFP、AFU、CA19-9单个肿癌标志物诊断PHC的敏感性分别为65.7%、87.1%、50.8%,特异性分别为92%、62%、78%;3种肿瘤标志物联合检测在PHC诊断中的敏感性和特异性分别为98.6%和98%,联合检测的敏感性与单项检测差异极有统计学意义(P<0.01).结论:AFP、AFU、CA19-9联合检测对PHC有一定的诊断价值,尤其可提高AFP阴性PHC的诊断率.

  17. AFP: Et reklamefossil er genfødt?

    DEFF Research Database (Denmark)

    Andersen, Lars Pynt

    2010-01-01

    Skjult sponsering vinder frem igen, og selv om det har en lang historie, ser det ud til at være mere skjult i de nutidige former end i de klassiske fra radiotiden. http://videnskab.dk/content/dk/blogs/reklamebloggen/afp_et_reklamefossil_er_genfodt   Udgivelsesdato: 7.6....

  18. 胎肝源性细胞促进人骨髓间充质干细胞向肝细胞的定向分化%Fetal liver-derived cells stimulate differentiation of human mesenchymal stem cells into hepatocytes in vitro

    Institute of Scientific and Technical Information of China (English)

    汪泱; 车媛梅; 张一; 谢安; 崔苏萍; 娄远蕾

    2009-01-01

    Objective:To investigate effects of human fetal liver-derived cell (HFLC) on differentiation of human mesenchy-mal stem cells (MSCs) into hepatocytes. Methods:Fetal hepatocytes from medical abortion and adult human MSCs labeled with Hoechst 33342 were co-cultured directly and/or separately in a dual chamber dish. Cell morphology was observed under a phase microscope. Hepatocyte specific markers alpha-fetoprotein (AFP), cytokeratin (CK)18 and CK19 were identified by immunofluoresent staining and glycogen storage was detected by Periodic Acid-Schiff (PAS) staining at different time intervals. Results: In the direct co-culture system, immunofluorescent staining demonstrated that the differentiated cells had the ability of expressing AFP, CK18 and CK19, and increased with time of differentiation. In addition, the differentiated cells had functional properties of hepatocytes, such as glycogen storage, which was evaluated by PAS staining. This phenomenon did not appear when HFLC were separated from human MSCs in a dual chamber dish. Conclusion:The direct co-culture of HFLC with adult human MSCs may create a particularly favorable microenvironment for the transdifferentiation of the latter into hepatocyte-like cell, which may serve as a cell source for cell therapy of hepatic tissues.%目的:探讨人胎肝源性细胞(HFLC)对骨髓间充质干细胞(MSCs)体外诱导分化为肝系细胞的效应和机制.方法:药物流产的胎肝源性细胞和Hoechst33342标记的人MSCs,按培养方式不同,分为HFLC和MSCs直接接触共培养组及transwell共培养组,用倒置显微镜观察细胞形态,在不同时段用免疫细胞荧光法检测肝系细胞标志如AFP、 CK-18和CK-19并进行糖原染色.结果:在直接接触共培养组,免疫细胞荧光法显示分化细胞表达AFP、 CK-18和CK-19,并随分化时间延长表达量增加;同时,分化细胞具有贮存糖原功能,PAS染色显示分化细胞糖原染色阳性.而在transwell共培养组,分化细

  19. Determination of maternal serum α-fetoprotein (AFP) by a Polish AFP radioimmunoassay - a suitable screening method for the detection of neural tube defects

    International Nuclear Information System (INIS)

    230 double determinations of maternal serum α-fetoprotein (AFP) have been made using a Polish AFP-RIA. On this basis the applicability of this method as screening for neural tube defects has been proved. An additional comparison with a French RIA and an enzyme immunoassay (EIA) from the Serum-Werke Dessau confirmed equal accuracy. There were three pathological increased values of AFP. In one case an anencephalus was found and in the other a spina bifida. In the third case the value was normal at the control examination. General AFP screening in pregnant women is recommended. (author)

  20. La demanda de las AFP chilenas: 1993-2002

    OpenAIRE

    Iván Marinovic; Salvador Valdés

    2010-01-01

    This paper estimates the demand curves faced individually by each pension fund management firm that serves compulsory saving for old age in Chile, in 1993-2002. These firms –Administradoras de Fondos de Pensiones, AFP- sell their services by retail methods, contacting each client separately. The service is also a subscription service, so demand refers both to the inflow of new subscribers and the outflow of separations. The paper presents two specifications with different degrees of aggregati...

  1. Complementary Effect between AFP and AFP-IgM in the Process of Hepatocellular Carcinoma Diagnosis%AFP-IgM复合物联合AFP对诊断肝癌的互补作用

    Institute of Scientific and Technical Information of China (English)

    徐斌; 蒋敬庭; 吴昌平

    2010-01-01

    目的 探讨新型肿瘤标志物AFP-IgM免疫复合物和甲胎蛋白(AFP)对肝细胞肝癌(Hepatocellular carcinoma,HCC)诊断的互补作用.方法 用酶联免疫吸附法与电化学发光法同步检测113名正常人、85例HCC患者、23例肝硬化和63例肝炎患者血清AFP-IgM与AFP含量.应用ROC曲线确立AFP-IgM和AFP的最佳切割值分别为300 AU/ml和20 μg/L作为有诊断学意义的临界值.在此切割值下,对AFP诊断为阴性的患者血清进行AFP-IgM检测以及对AFP-IgM诊断为阴性的患者血清进行AFP检测,探讨两种诊断方法对HCC诊断的互补作用.结果 在AFP诊断为阴性的HCC患者中AFP-IgM诊断的敏感度为68.0%,特异度为83.6%,有效率为81.6%;在AFP-IgM诊断阴性的HCC患者中AFP诊断的敏感度为74.2%,特异度为91.7%,有效率为88.8%.结论 AFP与AFP-IgM联合检测将提高对HCC诊断的效率,AFP和AFP-IgM在HCC的诊断中具有较好的互补性.

  2. A study of a non-enzyme amperometric immunosensor for rapid detection of AFP based on carbon nanotubes modified glass carbon electrode%基于碳纳米管修饰的无酶型新型甲胎蛋白安培免疫传感器研究

    Institute of Scientific and Technical Information of China (English)

    贾立永; 郑磊; 干宁; Wen Wang; 王前

    2011-01-01

    目的 构建新型甲胎蛋白安培免疫传感器.方法 首先在玻碳电极(GCE)表面修饰一层羧基化碳纳米管(CNTs).然后利用带负电荷的DNA分子和带正电荷的硫堇之间的静电作用,层层自组装修饰硫堇以增强检测信号,然后利用硫堇的氨基固定纳米金,以便固定抗体,最后利用牛血清白蛋白封闭未结合位点.结果 修饰的碳纳米管能够显著地提高电极的导电性,利用层层组装技术修饰了5层硫堇.在优化的条件下(pH 7.0,温浴时间25 min),制作的甲胎蛋白免疫传感器线性范围在0.5~25 ng/ml内,检测限0.02 ng/ml.结论 成功利用层层自组装技术构建新型基于碳纳米管修饰的无酶型甲胎蛋白安培免疫传感器,该传感器灵敏度高,特异性好,有望成为原发性肝癌早期诊断的新方法.%Objective To construct a novel amperometric immunosensor for the detection of alpha-fetoprotein (AFP).Methods First,glass carbon electrode(GCE) was modified with multi-wall carbon nanotubes (CNTs) ,and then the positively charged thionine, as the mediator, was immobilized by the negatively charged DNA through the layer-by-layer method.nanoAu was used to immobilized anti-AFP,and BSA was used to block any remaining active sites.Results Carbon nanotubes can significantly enhanced the electric conductivity of the electrode and was modified with 5 layers of thionine.With the optimized experimental parameters (pH 7.0,incubation time is 25 min), the proposed immunosensor could detect AFP in a linear range from 0.5 to 25 ng/ml with a detection limit of 0.02 ng/ml at a signal-to-noise ratio of 3 (S/D = 3).Conclusion A non-enzyme amperometric immunosensor for Rapid Determination of AFP based on carbon nanotubes modified glass carbon electrode was successfully constructed through layer-by layer method and shows a good performance for determination of AFP.It provides a potential alternative tool for early diagnosis of primary liver cancer in clinical

  3. A compact SEOP 3He neutron spin filter with AFP NMR

    Science.gov (United States)

    Ino, Takashi; Arimoto, Yasushi; Shimizu, Hirohiko M.; Sakaguchi, Yoshifumi; Sakai, Kenji; Kira, Hiroshi; Shinohara, Takenao; Oku, Takayuki; Suzuki, Jun-ichi; Kakurai, Kazuhisa; Chang, Lieh-Jeng

    2012-02-01

    We developed AFP NMR in an aluminum container for polarized noble gas nuclei. The radio frequency magnetic field inside the aluminum container was designed from computer simulations. The polarization loss by the AFP spin flip of 3He was measured to be as low as 3.8×10-4. With this technique, a compact in-situ polarizing 3He neutron spin filter with AFP NMR is demonstrated.

  4. Physics prospects with the ALFA and AFP detectors

    CERN Document Server

    Hamal, Petr; The ATLAS collaboration

    2013-01-01

    The ATLAS ALFA and AFP detectors - the experimental challenge of measuring forward protons at the LHC. We will describe the experimental challenge presented by the measurement of the forward protons at the LHC. The ATLAS ALFA and AFP projects involve the deployment of detectors capable of detecting protons that emerge intact from the interaction with very small scattering angles. In the case of the ALFA detector, located at ±240m from the ATLAS interaction point, this measurement has to be performed using special high \\beta* beam optics. The detector chosen for the task is a nearly edgeless scintillating fibre tracker capable of making measurements at very low t – below 10-3 GeV2 – in the Nuclear Coulomb interference region. In order to reach these very small t-values we need to deploy the detector very near to the beam line utilizing Roman Pots. The main purpose of the ALFA detector is to measure the total p-p cross-section, a fundamental quantity in hadronic physics of importance to particle and to cos...

  5. The application of lentil lectin-reactive alpha-fetoprotein ratio in the differential diagnosis of primary liver cancer%甲胎蛋白异质体比率在原发性肝癌鉴别诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    赵晓玲; 王晶晶; 赵巧玉; 黄梁镔

    2016-01-01

    Objective To explore the application of lentil lectin‐reactive alpha‐fetoprotein ratio (AFP‐L3% ) applied in the dif‐ferential diagnosis between hepatitis B infection of primary liver cancer and benign liver disease .Methods We included 108 cases of chronic HBV infection ,including 50 cases of primary liver cancer ,42 cases of cirrhosis ,16 cases of chronic hepatitis .Chemilumines‐cence detection was used to detect alpha‐fetoprotein (AFP) and AFP‐L3 content ,AFP‐L3 and the ratio of AFP (AFP‐L3% ) was calculated .Results AFP≥400 ng/mL as primary liver cancer diagnostic threshold ,the sensitivity and specificity were 36% ,84% , when used AFP‐L3% ≥ 10% as primary liver cancer diagnostic threshold ,the sensitivity and specificity were 62% ,83% . Conclusion AFP‐L3% is a better clinical indicator to distinguish between primary liver cancer and benign liver disease .AFP‐L3%can be used as a clinical indicator to differential diagnosis between HBV infection of primary liver cancer and benign liver disease .%目的:探讨甲胎蛋白异质体比率(A FP‐L3%)在乙型肝炎感染原发性肝癌和良性肝病鉴别诊断中的应用。方法慢性乙型肝炎病毒(HBV)感染108例,其中原发性肝癌50例、肝硬化42例、慢性肝炎16例。运用化学发光法检测甲胎蛋白(AFP)及AFP‐L3水平,计算AFP‐L3%。结果以AFP≥400 ng/mL作为原发性肝癌诊断阈值时,灵敏度及特异度分别为36%、84%;以AFP‐L3%≥10%作为原发性肝癌诊断阈值时,灵敏度及特异度分别为62%、83%。结论 AFP‐L3%更能区分原发性肝癌和良性肝病。临床上可结合A FP‐L3%作为鉴别诊断 HBV感染原发性肝癌和良性肝病的指标。

  6. Amniocentesis

    Science.gov (United States)

    ... baby’s amniotic fluid for proteins like alpha fetoprotein (AFP). Measuring the amount of AFP can check if your baby has neural tube ... baby that becomes the brain and spinal cord. AFP levels are often higher if your baby has ...

  7. Spina Bifida

    Science.gov (United States)

    ... or concerns you have about this prenatal testing. AFP - AFP stands for alpha-fetoprotein (sounds like: al-fa– ... a simple blood test that measures how much AFP has passed into the mother’s bloodstream from the ...

  8. 血清α-L-岩藻糖苷酶与甲胎蛋白和糖类抗原19-9在原发性肝癌诊断中的作用%Serum α-L-fucosidase and alpha-fetoprotein and carbohydrate antigen 19-9 in the diagnosis of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    熊利华; 陈春琴; 张春峰; 方叶青

    2010-01-01

    目的 探讨血清α-L-岩藻糖苷酶(AFU)、甲胎蛋白(AFP)和糖类抗原19-9(CA19-9)在原发性肝癌(PHC)诊断中的作用.方法 分别测定PHC患者48例、肝硬化患者28例、乙型肝炎患者74例、其他肿瘤患者38例和正常人136例AFU、AFP和CA19-9含量.结果 AFU、AFP和CA19-9在PHC患者中的敏感性分别为79.2%、68.6%和62.5%,在AFP阴性的PHC患者中,AFU、CA19-9的敏感性分别为62.5%和75.0%,3项联合检测诊断PHC的敏感性为87.5%.结论 AFU、AFP、CA19-9 3项联合检测对提高原发性肝癌的诊断率有十分重要意义.

  9. Module production of the one-arm AFP 3D pixel tracker

    CERN Document Server

    Grinstein, S; Chmeissani, M; Dorholt, O; Foerster, F; Lange, J; Paz, I Lopez; Manna, M; Pellegrini, G; Quirion, D; Rijssenbeek, M; Rohne, O; Stugu, B

    2016-01-01

    The ATLAS Forward Proton (AFP) detector is designed to identify events in which one or two protons emerge intact from the LHC collisions. AFP will consist of a tracking detector, to measure the momentum of the protons, and a time of flight system to reduce the background from multiple proton-proton interactions. Following an extensive qualification period, 3D silicon pixel sensors were selected for the AFP tracker. The sensors were produced at CNM (Barcelona) during 2014. The tracker module assembly and quality control was performed at IFAE during 2015. The assembly of the first AFP arm and the following installation in the LHC tunnel took place in February 2016. This paper reviews the fabrication process of the AFP tracker focusing on the pixel modules.

  10. Psc-AFP, an antifungal protein with trypsin inhibitor activity from Psoralea corylifolia seeds.

    Science.gov (United States)

    Yang, Xingyong; Li, Jun; Wang, Xiaowen; Fang, Weiguo; Bidochka, Michael J; She, Rong; Xiao, Yuehua; Pei, Yan

    2006-07-01

    An antifungal protein designated as Psc-AFP, with an apparent molecular mass of 18kDa, was isolated from a traditional Chinese herb, malaytea scurfpea (Psoralea corylifolia L.). The isolation procedure entailed extraction, cation exchange chromatography on CM FF, gel filtration chromatography on Superdex 75 and reversed-phase high performance liquid chromatography on SOURCE 5RPC column. Automated Edman degradation determined the partial N-terminal sequence of Psc-AFP to be NH2-EWEPVQNGGSSYYMVPRIWA, which displayed homology with plant trypsin inhibitors. The protease inhibitor activity of Psc-AFP was then confirmed by the inhibition on trypsin. Psc-AFP at 10 microM inhibited the mycelial growth of Alternari brassicae, Aspergillus niger, Fusarium oxysporum and Rhizoctonia cerealis, suggesting that Psc-AFP has a role in the defense against pathogens.

  11. Diagnostic value of Joint Detection of GP73 and AFP-L3 in Primary Hepatic Carcinoma with Low Concentration of AFP

    Directory of Open Access Journals (Sweden)

    Lei CAI

    2015-03-01

    Full Text Available Objective: To explore the applicative value of serum Golgi protein (GP73 and alpha-proteinvariant (AFP-L3 in the diagnosis of patients with primary hepatic carcinoma (PHC. Methods:Totally 110 patients were enrolled, including 60 PHC patients with low concentration of AFP ((1.1-108.0 μg/L and 50 patients with non-PHC digestive system diseases (20 cases of patients with chronic hepatitis, 15 patients with liver cirrhosis, 4 patients with bile duct cancer, 4 patients with gastric cancer, 4 patients with rectal cancer and 3 patients with colon cancer. In addition, 42 healthy people were selected as control group. GP73 was detected by enzyme-linked immunosorbent assay (ELISA trace centrifugal column method was adopted for separation of AFP-L3. Luo's chemiluminescence method was used to determine the total content of AFP and AFP-L3 in eluent for calculating the ratio of AFP-L3/ AFP. Results: The levels of serum GP73 and AFP-L3(% in PHC group were significantly higher than the other 2 groups (P<0.01 and the level of serum GP73 and AFP-L3 (% in Non-PHC group were higher than those in healthy group (P<0.01. ROC analysis showed that the area under ROC curve of single GP73 and AFP-L3(% in diagnosis of PHC and non-PHC were 0.887 and 0.860, respectively. Additionally, the ROC analysis also showed that critical value of GP73 and AFP-L3 for the diagnosis of HPC were 83.78 μg/L and 13.87%, respectively. The sensitivity of joint detection of serum GP73 or AFP-L3 was higher than detection of them alone (90.0 vs. 71.7 and 60.0, P<0.05 but the specificity was similar between single detection and joint detection (P>0.05. The positive predictive value and negative predictive value of joint detection of them was higher than single detections of them and there was significant differences in negative predictive value (P<0.05 but no difference in positive predictive value (P>0.05. The overall response rate of joint detection of GP73 and AFP-L3 was higher

  12. Diagnostic value of ornithine carbamoyltransferase/alanine aminotransferase and alpha fetoprotein in hepatocellular carcinoma%鸟氨酸氨甲酰基转移酶/丙氨酸氨基转移酶和甲胎蛋白联合检测在肝细胞癌中的诊断价值

    Institute of Scientific and Technical Information of China (English)

    梁彬; 程大也; 孔虹; 解芳

    2008-01-01

    目的:研究鸟氨酸氨甲酰基转移酶/丙氨酸氨基转移酶(OCT/ALT)比值和甲胎蛋白(AFP)联合检测对肝细胞癌的诊断价值.方法:测定61例肝细胞癌、49例慢性肝炎、45例肝硬化和51名健康体检者血清OCT/ALT比值和AFP水平,应用受试者工作特征曲线(ROC)进行评价.结果:肝细胞癌组血清OCT/ALT和AFP水平高于对照组、肝炎组和肝硬化组,差异有统计学意义(P<0.05).血清OCT/ALT和AFP的ROC曲线下面积分别为0.808和0.758.OCT/ALT的最优截断点约为3.2,灵敏度和特异度为68.3%和90.3%.AFP的最优截断点约为400ng/ml,灵敏度和特异度分别为60.7%和80.0%.结论:通过ROC曲线评价,在肝细胞癌的诊断中,血清0CT/ALT和AFP联合检测优于两项指标的单独检测.

  13. 体外诱导脂肪源性干细胞向类肝细胞的定向分化%Directional differentiation of adipose tissue-derived stem cells into hepatic-like cells in vitro by procedure-induction

    Institute of Scientific and Technical Information of China (English)

    刘剑; 李立; 冉江华; 张升宁; 邵剑春

    2011-01-01

    BACKGROUND: How to establish effective stable hepatic cell differentiation schedule, to purify or rapidly amplify stable hepatic-like cells needs to be solved before adipose tissue-derived stem cells (ADSCs) were used to treat liver diseases. OBJECTIVE: To establish sequencing induction system of ADSCs transforming into hepatic-like cells.METHODS: After isolated and purified the Lewis rat ADSCs, the surface marks of ADSCs were identified by flow cytometry. The rat ADSCs were transformed into the hepatic-like cells in the procedure-culture system by tris-step including hepatocyte growth factor (FGF), fibroblast growth factor-4, acid fibroblast growth factor and oncostatin M cytokine. RESULTS AND CONCLUSION: After rat ADSCs were induced at 7, 14 and 21 days, the expression of albumin (ALB), alpha fetoprotein (AFP) and CK18 mRNA was determined and fortified over time. Hepatic-like cells had ALB synthesis function. Metabolism of ammonia and urea synthesis occurred and lasted after 9-12 days. Results suggest ADSCs were successfully differentiated into hepatic-like cells in vitro after procedure-induction.%背景:用脂肪源性干细胞治疗肝脏疾病之前,如何建立有效稳定的肝细胞分化诱导方案,纯化并快速扩增性能稳定的类肝细胞等问题亟待解决.目的:建立大鼠脂肪源性干细胞转化为类肝细胞的程序化诱导体系.方法:分离纯化Lewis大鼠脂肪源性干细胞,流式细胞仪鉴定其表面标志,分3个阶段加入含有肝细胞生长因子、成纤维细胞生长因子4、酸性成纤维细胞生长因子、制瘤素M细胞因子的诱导培养体系,使脂肪源性干细胞向肝细胞转化.结果与结论:大鼠脂肪源性干细胞诱导7,14,21 d后,细胞阳性表达 ALB、AFP、CK18mRNA,表达量随诱导时间延长而增强,类肝细胞具有白蛋白合成功能.氨代谢和尿素的合成功能在9~12 d出现并持续存在.结果表明脂肪源性干细胞体外分段诱导可成功转化为类肝细胞.

  14. AFP and ALFA detectors as a momentum spectrometer system - feasibility study

    CERN Document Server

    ABDUL KHALEK, Rabah

    2015-01-01

    We consider a possibility to measure a leading proton momentum using simultaneously the AFP and ALFA detectors. The idea is based on the simple fact that the proton trajectory through the magnetic field between the AFP and ALFA$_{1}$ detectors, depends on the proton's momentum loss $\\xi$. The feasibility of the proton momentum measurement is directly related to the availability of the magnetic fields and their configuration, the overlap between ALFA and AFP detectors and their resolutions as well as energy losses and the multiple scattering effect that could wash away little actions from the magnets on the proton.

  15. a-L-岩藻糖苷酶与甲胎蛋白联合检测对原发性肝癌诊断的临床评价%Clinical evaluation of combined AFU and AFP detection in the diagnosis for patients with PHC

    Institute of Scientific and Technical Information of China (English)

    殷建立; 周秦蜀; 段仲壁; 韩峰

    2007-01-01

    目的 探讨a-L-岩藻糖苷酶(a-L-fucosidase,AFU)与甲胎蛋白(alpha fetoprotein,AFP)联合检测在原发性肝癌诊断中的临床意义.方法 对1992年~2000年在普外科和消化内科门诊及住院的358例原发性肝癌,628例良性肝病,52例肝癌介入化疗术后患者,28例转移性肝癌,81例其他肿瘤,251例正常对照者,以AFU>600(nmol/ml.h)为阳性标准,用酶标法检测.以AFP>20(ng/ml)为阳性标准,用放免法检测.绘制受试者工作特性曲线(receiver operating characteristic curve,ROC)并计算曲线下面积评价诊断价值.结果 原发性肝癌(primary hepatic carcinom,PHC)组血清AFU水平显著高于其他各组(P<0.01),AFP水平与慢性肝病组、其他肿瘤组、正常对照组相比差异有统计学意义(P<0.05).PHC组和转移性肝癌组中AFU的检出率与AFP的检出率相比差异有统计学意义(P<0.05).结论 AFU可作为诊断PHC的一种较好的血清肿瘤标志物.AFU与AFP联合检测,可提高诊断PHC的敏感性,并可作为对PHC的疗效观察的一项指标.

  16. Diagnostic Value of Serum AFU,AFP and TK1 for Primary Liver Cancer with Logistic Regression and ROC Curve%ROC曲线及Logistic回归评价血清AFU,AFP和TK1在原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    李步任; 张惠冰; 张诗颜

    2015-01-01

    目的 探讨血清α-L-岩藻糖苷酶(α-L-fucosidase,AFU)、甲胎蛋白(alpha-fetoprotein,AFP)和胸苷激酶1(thymidine kinase1,TK1)在原发性肝癌(primary liver cancer,PLC)诊断中的临床意义.方法 测定114例PLC患者、105例良性肝病患者血清AFU,AFP和TK1水平,并与同期102例健康正常对照的血清浓度水平进行比较.用Logistic回归模型绘制ROC曲线并计算曲线下面积(AUCROC)以评价各指标的诊断价值.结果 三组间数据呈非正态分布(KolmogorovSminov检验,Z=0.100~0.517,均P<0.001).三组数据比较差异有统计学意义(Kruskal-Wallis检验,x2=67.230~104.442,均P<0.001).PLC组和良性肝病组患者比较,PLC组血清AFU,AFP和TK1水平高于良性肝病组,差异有统计学意义(Mann-Whitney U检验,Z=-8.772~2.077,P<0.05).良性肝病组AFU高于正常对照组,差异有统计学意义(Mann-WhitneyU检验,Z=-7.912,P<0.001).PLC组与非肝癌组诊断性能评价ROC曲线下最佳cut-off值:AFU为40.75 U/L,AFP为10.86 μg/L和TK1为1.92 pmol/L;ROC曲线下的面积AFU为0.725,AFP为0.844,TK1为0.772,联合检测曲线下面积为0.900,其约登指数(70.5%)和敏感度(86.0%)最高.结论 血清AFU,AFP和TK1联合检测对PLC的诊断具有重要价值,并且可显著增加PLC诊断的约登指数和敏感度.

  17. 门静脉高压时经脾移植骨髓间充质干细胞的分化与迁移%Bone marrow mesenchymal stem cells transplantation via spleen as a treatment for terminal liver disease in rats with portal hypertension

    Institute of Scientific and Technical Information of China (English)

    孙松; 陈功; 郑珊

    2011-01-01

    Objective To study the efficacy of bone marrow mesenchymal stem cells (BMMSC)transplantation via spleen as a treatment for terminal liver disease in rats with portal hypertension.Methods Bone marrow mesenchymal stem cells of Sprague-Dawley rats were isolated and culture ex vivo in the medium containing hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4).The expressions of alpha-fetoprotein (AFP) and cytokeratin 18 (CK-18) were detected by immunofluorescence staining in the fifth passage cells.Recipient rats were randomly divided into the experimental group (the common bile ducts were ligated and cut off) and the control group (underwent sham operations).The fifth passage cells were labeled with CM-Dil,and were transplanted via spleen injection.The BMMSC distribution was observed under the fluorescence microscope The integrated optical density (IOD) and cell distribution score were quantitated using the Image pro-Plus 6.0.Results After co-incubated with HGF and FGF-4,the Golgi apparatus,endoplasmic reticulum,ribosomes,and mitochondria were significantly increased in the 5th passage BMMSC cells.The immunofluorescence staining of the AFP and CK-18 found that the cells expressed both of the proteins.The CM-Dil staining could also be observed in BMMSC.And the CM-Dil labeling rate was as high as 99.5%.The integrated optical density (IOD) of transplanted cells in the experimental group was significantly higher than that of the control group (1 130 067 ± 209 464 vs 293 505 ± 88 383,P<0.01).In addition,the cell distribution score in the experimental group was lower than that of the control group (2.36 ±0.27 vs 1.99 ± 0.36,P<0.05).Conclusions HGF and FGF-4 can induce bone marrow mesenchymal stem cells differentiate into the hepatocyte-like cells ex vivo.The BMMSC cells can migrate into the liver of the rats with portal hypertension via spleen injection.%目的 观察体外诱导骨髓间充质干细胞(BMSC)能否向肝样细胞分化,探讨经

  18. 1421例孕中期妇女AFP和Free-βHCG联合检测的产前筛查结果分析%The Analysis Results of Prenatal Screening of Combined Detection of the Second Trimester Women AFP and Free-βHCG in 1421 Cases

    Institute of Scientific and Technical Information of China (English)

    凌显桢; 覃人记

    2013-01-01

      目的探讨孕中期(14~21周)妇女血清甲胎蛋白(AFP)和游离β-人绒毛膜促性腺激素(Free-βHCG)双联法检测在早期诊断唐氏综合征胎儿缺陷的临床应用价值。方法采用化学发光免疫分析法分别对1421例孕中期妇女血清进行AFP和Free-βHCG两项指标检测,结合妇女年龄、孕周、体质量等临床资料,通过配套的风险评估软件计算出该妇女的唐氏综合征(DS)风险率。对DS、18-三体综合征高风险的孕妇建议其进一步进行羊水穿刺产前诊断,对神经管缺陷(NTD)高风险的孕妇进行超声波检查确诊。结果在筛查的1421例孕妇中,高风险的孕妇为104例,阳性率为7.32%;其中DS高危78例占5.49%,经确诊有4例为阳性;NTD高危14例占0.99%,经确诊有2例为阳性;18-三体高危12例占0.84%,经确诊阳性率为0。结论利用孕中期妇女AFP和Free-βHCG两项指标的检测,有效地筛查出了本地区DS高风险孕妇,降低了DS缺陷儿的出生率,在减轻家庭及社会负担起到了重要的作用。%Objective Study of second trimester(14-21weeks) women serum alpha-fetoprotein(AFP) and free beta human chorionic gonadotropin(Free-βHCG) duplex assay in early diagnosis of fetal defects in Down,s syndrome clinical application value. Methods In 1421 pregnant women serum two indicators of AFP and Free-βHCG was detected by chemiluminescence immunoassay method, combined with clinical data, women,s age, gestational age, body weight, through the risk assessment software suppurting the calculation of the women of Down,s syndrome(DS) risk rate. On DS, 18-trisomy syndrome high risk pregnant women suggest the further amniocentesis in prenatal diagnosis of neural tube defects, (NTD)high risk pregnant women were ultrasonic diagnosed. Results In 1421 cases of pregnant women screening in high risk pregnant women, 104 cases, the positive rate was 7.32%;of which 78 cases of high-risk DS

  19. Physics prospects with the ALFA and AFP detectors

    CERN Document Server

    Hamal, P; The ATLAS collaboration

    2013-01-01

    We will describe the experimental challenge presented by the measurement of the forward protons at the LHC. The ATLAS ALFA and AFP projects involve the deployment of detectors capable of detecting protons that emerge intact from the interaction with very small scattering angles. In the case of the ALFA detector, located at $\\pm${240}~m from the ATLAS interaction point, this measurement has to be performed using special high $\\beta^*$ beam optics. The detector chosen for the task is a nearly edgeless scintillating fibre tracker capable of making measurements at very low $t$ - below $10^{-3} \\text{GeV}^2$ - in the Nuclear Coulomb interference region. In order to reach these very small $t$ values we need to deploy the detector very near to the beam line utilizing Roman Pots. The main purpose of the ALFA detector is to measure the total p-p cross-section, a fundamental quantity in hadronic physics of importance to particle and to cosmic ray physicists and to provide absolute measurement of luminosity which includ...

  20. Cyst and tumor of liver

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    2008081 Construction of the alpha-fetoprotein enhancer controlled gene therapy vector specific for hepatocellular carcinoma and identification of its activity. MA Xiaojuan(马晓娟), et al. Dept Nucl Med, West China Hosp, Sichuan Univ, Chengdu 610041. World Chin J Digestol 2007;15(33):3474-3478. Objective To construct a gene-modified hepatocellular carcinoma (HCC)-specific luciferase expression vector regulated by alpha fetoprotein (AFP) p

  1. 肝癌特异性AFP亚组分联合AFP-mRNA对肝癌诊断与鉴别价值%Clinical values of combined hepatoma-specific α-fetoprotein (AFP) fraction and AFP-mRNA determination in diagnosis and differentiation of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    吴玮; 姚登福; 袁咏梅; 樊冀伟; 陆秀凤; 李小华; 邱历伟; 吴信华

    2006-01-01

    目的探讨甲胎蛋白(AFP)基因及肝癌特异性AFP亚组份(AFP-L3)在肝癌早期诊断和鉴别诊断中的价值.方法从肝病患者外周血中制备总RNA,以逆转录聚合酶链反应(RT-PCR)扩增AFP基因.用小扁豆凝集素亲和层析法分离血清中AFP-L3,放射免疫法测定AFP及AFP-L3.结果在肝癌和肝硬化病人中AFP-L3阳性率分别为91.7%(110/120)和18%(9/50),AFP-mRNA阳性率分别为56.7%(68/120)和16%(8/50),两者总阳性率分别为95%(114/120)和26%(13/50).正常对照组AFP-L3及AFP-mR-NA均为阴性.肝癌病人AFP-L3%及AFP-mRNA阳性率明显高于肝硬化及正常对照组(P<0.01).AFP-L3及AFP-mRNA与肿瘤大小、数目等无关(P>0.05),而与肿瘤分化程度、是否转移及有无复发等明显相关(P<0.05).结论外周血AFP-L3及AFP-mRNA检测特异性强、敏感性高,有助于肝癌诊断与鉴别诊断、肝外转移或术后复发监测.

  2. Local production and evaluation of primary reagents for immunoradiometric assay of AFP

    International Nuclear Information System (INIS)

    A rapid multisite immunoradiometric assay for measurement of human α-fetoprotein (AFP) that uses a high affinity monoclonal and polyclonal antibodies directed against distinct and separate determinants on the protein was developed and designated as coated-tubes IRMA (CT-IRMA). AFP standards or serum samples were added into the polystyrene tubes coated with goat IgG antibody against human AFP. After 1 hour incubation, the tubes were washed and a radioiodinated mouse monoclonal antibody was added and then incubated overnight at room temperature. The sensitivity of the CT-IRMA was found to he 0.26 ng/ml. The recovery of AFP mixed with human serum was 98.1 -111.1 %. The within-assay, and between-assay coefficients of variation were 1.49 - 3.11 % and 4.89 - 7.44 %, respectively. The assay correlated well with a commercial method (CIS bio international, France) ( r = 0.9741, n = 83, OAEP kit 0.8748 CIS kit - 2.1561). The mean concentration of AFP in normal serum was 5 ng/ml. This developed kit reagents are being assessed clinically in multicenter study at National Cancer Institute, Chulalongkorn Hospital and Pramongkutklao Hospital to find the real clinical application in various groups of patients which include early detection monitoring therapy, follow up of hepatocellular carcinoma, early identification and monitoring of AFP- producing tumors in high-risk populations. (Author)

  3. Salvage therapy in patients with germ cell tumors.

    Science.gov (United States)

    Einhorn, Lawrence H

    2015-01-01

    Testicular cancer is the most curable metastatic solid tumor. Initial chemotherapy is evidence based with risk stratification into three prognostic categories: good, intermediate, and advanced disease. Guidelines for disease management following progression after initial cisplatin combination chemotherapy are less clear. Options include salvage surgery for patients with anatomically confined relapse, standard-dose cisplatin combination chemotherapy, or high-dose chemotherapy with carboplatin plus etoposide with peripheral blood stem cell transplantation. Proper interpretation of a presumed relapse can be complicated. Growing masses on imaging studies might reflect a growing teratoma. Persistent elevations of serum human chorionic gonadotropin (hCG) or alpha fetoprotein (AFP) are only an indication for salvage therapy if there is a definitive rise in the tumor marker. Elevated and rising serum hCG as the only evidence of recurrence can be because of cross reactivity with luteinizing hormone or usage of marijuana rather than progressive cancer. Elevated liver function tests can cause rising serum AFP. PMID:25993183

  4. The value of combine determination of AFP variants and AFP in the diagnosis of primary liver cancer%AFP和AFP异质体对405例原发性肝癌的诊断价值探讨

    Institute of Scientific and Technical Information of China (English)

    王爱华; 王华梁; 方芳; 邓海琳; 马莉苓; 李梅君

    2002-01-01

    目的探讨甲胎蛋白(AFP)和甲胎蛋白异质体(AFP Variants,AFP-V)对原发性肝癌(PHC)的诊断价值.方法用酶联免疫吸附试验(ELISA)和交叉免疫电泳、放射性自显影法分别检测AFP和小扁豆凝集素亲和性甲胎蛋白异质体(LCA-AFP-V)的血清含量.结果 405例PHC患者中,AFP阳性占75.8%,LCA-AFP-V阳性占54.3%;210例AFP低浓度阳性患者中,LCA-AFP-V阳性125例,而97例AFP高浓度阳性患者,有95例伴有异质体的升高;98例AFP阴性患者中,35例LCA-AFP-V阳性;223例非PHC中AFP阳性占42.2%,LCA-AFP-V阳性占25.6%.结论单独应用AFP或AFP-V均有一定的局限性,两者联合使用将有助于提高原发性肝癌的检出率.

  5. 甲胎蛋白(AFP)与原发性肝癌早期诊断的关系%Armor embryo protein (AFP) and primary liver cancer early diagnosis relations

    Institute of Scientific and Technical Information of China (English)

    宋光; 刘晶; 张男

    2008-01-01

    甲胎蛋白(AFP)是有卵黄囊及胚胎产生的糖蛋白,是肝细胞癌重要的肿瘤标志物.肝细胞癌变时AFP基因常被激活而强烈表达,转录成AFP mRNA和翻译成AFP,因此根据AFP mRNA的表达情况可判断AFP基因的活化情况,为原发性肝癌的诊断提供了可靠的依据,为临床治疗争取了时间.

  6. AFP-L3阳性肝癌患者肝动脉化疗栓塞术后碘油沉积与AFP-L3%下降相关性分析

    Institute of Scientific and Technical Information of China (English)

    尤慧明; 赵念博; 陈祖华

    2014-01-01

    目的:探讨甲胎蛋白异质体L3(AFP-L3)阳性肝癌肝动脉化疗栓塞术(TACE)术后碘油沉积与AFP-L3占总AFP 的比例(AFP-L3%)下降的相关性。方法随机选择AFP-L3阳性(即≥10%)并行TACE治疗的肝癌68例,术前1周和术后4周分别行AFP-L3检测。根据术后4周CT碘油沉积比例进行分级,与AFP-L3%术后下降及转阴率行相关性分析。结果 CT碘油沉积比例分级及AFP-L3%转阴情况:Ⅰ级13例(19.1%),AFP-L3%转阴3例(23.1%,3/13);Ⅱ级19例(28.0%),AFP-L3%转阴11例(57.9%,11/19);Ⅲ级21例(30.9%),AFP-L3%转阴15例(71.4%,15/21);Ⅳ级15例(22.1%),AFP-L3%转阴14例(93.3%,14/15)。AFP-L3%总转阴43例(63.2%),各级别AFP-L3%转阴率比较差异有统计学意义。结论 AFP-L3阳性肝癌TACE术后碘油沉积比例越高,AFP-L3%下降率及转阴率越高。

  7. Union examination of AFP, AFU, AFPL3 andγ-GT in early diagnosis of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    Yuan Shao; Zuo-ren Wang; Lei Shi

    2009-01-01

    Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP), crag algae glycosidase (AFU), armor embryo protein heteroplasmon (AFPL3) andγ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results Finally among the 30 patients in the early liver cancer group, 23 were positive with AFP, 20 with AFU, 15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP, AFPL3, AFU and γ-GT at the same time; 5 with AFP, AFPL3 and γ-GT; 5 with AFP, AFU and AFPL3; 7 with AFP, AFU andγ-GT. By contrast, in the control group, among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history, 11 were found positive with AFP, 3 with AFPL3, 12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP, AFPL3, AFU and γ-GT in the blood serum at the same time. Conclusion The union examination of AFP, AFU, AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.

  8. Clinical significance and influencing factors of serum level of AFP,uE3 and Free β-hCG in woman at intermediate stage of pregnancy%中期孕妇血清中AFP、uE3、游离β-hCG检测的临床意义及影响因素分析

    Institute of Scientific and Technical Information of China (English)

    刘爱胜; 陈荣贵; 文艳

    2011-01-01

    Objective To investigate the clinical significance and influencing factors of serum level of alpha fetoproteinC AFP) , free estriol(Ue3) and free (3-human chorionic gonadotropin(free (3-Hcg) in woman at intermediate stage of pregnancy. Methods Completely automatic immunity illumination analysis was performed for the detection of serum AFP,Ue3 and free-(3-HCG in 5 308 cases of woman at 14 - 20 week during pregnancy. The diagnostic criterion of high-risk was 1 : 380 for Down's syndrome(DS) ,1 : 334 for Trisomy 18 and 1 : 1 000 for open spina bifida (OSB). Amniotic fluid puncture or ultrasound inspection were performed for high-risk group. Follow-up visit was carried out for all 5 308 cases of pregnant women during whole process of pregnancy. Results Among all subjects, 5. 2%(275/5 308) were positive for DS screening, among which 5. 8% C16/275) were definitely diagnosed for DS by amniotic fluid puncture, ultrasound inspection or follow-up visit after parturition. In low-risk group, 3 cases were definitely diagnosed for DS with the false negative rate of 0. 06% (3/5 033). Subjects in high-risk group were mainly above 35 years old, and the positive rate of screening increased with the raise of age. There was significant difference of positive rate of screening between pregnant women more than 35 years old[12. 9%(174/1 345)] and those less than 35 years old[2. 5% C101/3 963)] (P0. 05). The difference of incidence rate of DS during high-risk and low-risk group was significant (P0.05).高危、低危组间唐氏综合征发病率比较,两组差异有统计学意义(P<0.01).结论 中期孕妇血清中AFP、uE3、游离β-hCG指标联合检测是筛查唐氏综合征、18-三体综合征、开放性脊柱裂准确且无创伤性的方法,可作为较理想的产前检查模式,但有一定的假阳性率.为了减少假阳性,高危孕妇要及时进行羊水和B超检查.孕妇年龄与检出阳性率呈正相关.因此,建议在30岁前计划生育,减少或杜绝高危出生的缺陷患儿.

  9. [A case of AFP and PIVKA-II producing gastric carcinoma].

    Science.gov (United States)

    Tanaka, Hiroki; Ishii, Suguru; Akutsu, Noriyuki; Ohashi, Hirokazu; Tokuno, Tetsuya; Suzuki, Takashi; Okuda, Hiroyuki; Shinomura, Yasuhisa; Imai, Kohzoh

    2006-04-01

    A 71-year-old man was admitted to our hospital with edema of the ambilateral crura and liver dysfunction. Serum levels of AFP and PIVKA-II were both abnormally high. An abdominal CT revealed many low density masses in both lobes of the liver. Endoscopic examination and gastric radiography showed a Type 1 tumor in the fornix of the stomach. Biopsy specimen showed poorly differentiated adenocarcinoma. Immunohistochemical stains demonstrated the presence of AFP and PIVKA-II in tumor cells. A diagnosis of AFP and PIVKA-II producing gastric carcinoma with liver metastasis was made and the internal use of S-1 was started. The result of treatment was determined to be partial response. However, the condition of his entire body gradually worsened and he died of hepatic insufficiency.

  10. Synergistic Activity of the Plant Defensin HsAFP1 and Caspofungin against Candida albicans Biofilms and Planktonic Cultures.

    Directory of Open Access Journals (Sweden)

    Kim Vriens

    Full Text Available Plant defensins are small, cysteine-rich peptides with antifungal activity against a broad range of yeast and fungi. In this study we investigated the antibiofilm activity of a plant defensin from coral bells (Heuchera sanguinea, i.e. HsAFP1. To this end, HsAFP1 was heterologously produced using Pichia pastoris as a host. The recombinant peptide rHsAFP1 showed a similar antifungal activity against the plant pathogen Fusarium culmorum as native HsAFP1 purified from seeds. NMR analysis revealed that rHsAFP1 consists of an α-helix and a triple-stranded antiparallel β-sheet stabilised by four intramolecular disulfide bonds. We found that rHsAFP1 can inhibit growth of the human pathogen Candida albicans as well as prevent C. albicans biofilm formation with a BIC50 (i.e. the minimum rHsAFP1 concentration required to inhibit biofilm formation by 50% as compared to control treatment of 11.00 ± 1.70 μM. As such, this is the first report of a plant defensin exhibiting inhibitory activity against fungal biofilms. We further analysed the potential of rHsAFP1 to increase the activity of the conventional antimycotics caspofungin and amphotericin B towards C. albicans. Synergistic effects were observed between rHsAFP1 and these compounds against both planktonic C. albicans cells and biofilms. Most notably, concentrations of rHsAFP1 as low as 0.53 μM resulted in a synergistic activity with caspofungin against pre-grown C. albicans biofilms. rHsAFP1 was found non-toxic towards human HepG2 cells up to 40 μM, thereby supporting the lack of a general cytotoxic activity as previously reported for HsAFP1. A structure-function study with 24-mer synthetic peptides spanning the entire HsAFP1 sequence revealed the importance of the γ-core and its adjacent regions for HsAFP1 antibiofilm activity. These findings point towards broad applications of rHsAFP1 and its derivatives in the field of antifungal and antibiofilm drug development.

  11. The ATLAS ALFA and AFP detectors - the experimental challenge of measuring forward protons at the LHC

    CERN Document Server

    Trzebinski, M; The ATLAS collaboration

    2013-01-01

    The Absolute Luminosity For ATLAS (ALFA) and ATLAS Forward Protons (AFP) detectors are introduced. The forward proton trajectories are shown. The similarities and differences between these two detector systems are described. Finally, the physics possible to be done in these forward detectors is discussed. In particular, in case of ALFA the elastic scattering and exclusive di-pion production are described. In case of AFP, the hard diffractive processes like: Single Diffractive Jet, Double Pomeron Exchange Jet, Exclusive Jet and anomalous coupling production are described.

  12. Effects of combined detection of AFP and HCCR-1 in diagnosis of small hepatocellular carcinoma%AFP 和 HCCR-1联合检测在诊断小肝癌中的作用

    Institute of Scientific and Technical Information of China (English)

    王欣; 齐川; 李满元

    2014-01-01

    目的:探讨 AFP 和 HCCR-1联合检测在诊断小肝癌中的作用。方法:检测肝癌患者体内 AFP 和 HCCR-1表达水平。结果:1258例肝癌患者血清中 AFP 和 HCCR-1的检测结果:AFP 检测阳性率是576(46%),HCCR-1检测的阳性率是646(51.3%)。当 AFP 和 HC-CR-1联合检测时阳性率提高到74.1%。 AFP 和 HCCR-1分别检测小肝癌(直径小于2厘米)的阳性率是40.1%和23.4%,二者联合检测时阳性率上升为56.9%。结论:实验结果表明 HCCR-1可以作为检测肝癌的有用标志物,当 AFP 和 HCCR-1结合在一起检测,能提高小肝癌的阳性率。%Objective:To discuss effects of combined detection of AFP and HCCR-1 in diagnosis of small hepatocellular carci-noma. Methods:The levels of AFP and HCCR-1 in the patients with liver cancer were detected. Results: Of the 1258 cases of liver cancer patients, 576 (46% ) and 646 (51. 3% ) cases were positive for AFP and HCCR-1, respectively. In the combined detection of AFP and HCCR-1, the positive rate for liver cancer increased up to 74. 1% . The positive rate for small hepatocellular carcinoma (di-ameter <2 cm) increased to 56. 9% in the combined detection of AFP and HCCR-1, although those were only 40. 1% and 23. 4% of AFP ad HCCR-1, respectively. Conclusions: It suggests that the HCCR-1 is a useful biomarker for liver cancer. The positive rate of small hepatocellular carcinoma could be significantly improved in the combined detection of HCCR-1 and AFP.

  13. The value of Golgi protein 73 detection combined with alpha-fetoprotein,α-L-fucosidase,α-antitrypsin in the diagnosis of primary liver cancer%高尔基体蛋白73联合甲胎蛋白、α-L 岩藻糖苷酶、α-抗胰蛋白酶检测对原发性肝癌的诊断价值

    Institute of Scientific and Technical Information of China (English)

    黄华; 梁红梅; 胥慧; 周庭雄; 王建蔚

    2015-01-01

    Objective To study the diagnostic value of the golgi protein 73 (GP73) combined with alpha‐fetoprotein (AFP) ,α‐L‐fucosidase (AFU) andα‐antitrypsin(α‐AT) detection in primary hepatic carcinoma(PHC) .Methods 100 patients with PHC ,100 patients with hepatitis and cirrhosis ,and 100 healthy people hospitalized in the hospital from October 2011 to May 2013 were en‐rolled in the study .Serum AFP levels were detected by using Beckman DxI800 chemiluminescent analyzer ,serum AFU ,α‐AT levels were detected by using Beckman DxC800 automatic biochemical analyzer ,and GP73 levels were measured by using enzyme‐linked immunosorbent GP73 quantitative detection kits .Results In patients with PHC and patients with hepatitis and cirrhosis ,serum GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than healthy people(P<0 .05) .In patients with PHC ,GP73 ,AFP ,AFU ,α‐AT levels were significantly higher than those in patients with hepatitis and cirrhosis(P<0 .05) .The sensitivities of single serum GP73 ,AFP ,AFU ,α‐AT detection were 75% ,72% ,23% ,67% respectively and specificity were 95% ,95% ,100% ,97% respective‐ly .The sensitivity and specificity of the 4 tumor markers′combined detection were 99% and 100% .The sensitivity of combined de‐tection was significantly different from single detection(P<0 .05) .Conclusion GP73 ,AFP ,AFU ,α‐AT may improve the diagnostic efficiency of primary liver cancer .%目的:研究高尔基体蛋白73(GP73)联合甲胎蛋白(AFP)、α‐L岩藻糖苷酶(AFU)、α‐抗胰蛋白酶(α‐AT)检测对原发性肝癌诊断的价值。方法选取2011年10月至2013年5月在该院住院及就诊的原发性肝癌、肝炎和肝硬化患者及健康体检者各100例作为研究对象,使用Beckman DxI800化学发光分析仪检测血清AFP水平;用Beckman DxC800全自动生化分析仪检测血清AFU、α‐AT水平;用GP73酶联免疫定量检测试剂盒测定 GP73水平。结果原发性

  14. [A surgically resected case of AFP and PIVKA-II producing gastric cancer with hepatic metastasis].

    Science.gov (United States)

    Tomono, Ayako; Wakahara, Tomoyuki; Kanemitsu, Kiyonori; Toyokawa, Akihiro; Teramura, Kazuhiro; Iwasaki, Takeshi

    2013-05-01

    A 78-year-old man was admitted for workup for a liver tumor. Both serum AFP and PIVKA-II levels were high (2260ng/ml and 806mAU/ml, respectively). Contrast-enhanced CT scan and MRI using Gd-EOB-DTPA demonstrated a liver tumor in segment 6 resembling the imaging patterns of hepatocellular carcinoma (HCC), while the upper gastrointestinal endoscopy revealed a type 2 gastric cancer in the gastric antrum. Although the liver metastasis of the gastric cancer was undeniable, we performed partial resection of segment 6 of the liver and distal gastrectomy under a preoperative diagnosis of double cancer. Histopathologically, gastric tumor consisted of two components, such as well differentiated adenocarcinoma and hepatoid adenocarcinoma. The histology of the liver tumor was similar to that of the hepatoid component in the stomach lesion. Immunohistochemical staining revealed both the gastric and the liver tumors to be positive for AFP and PIVKA-II, yielding a definite diagnosis of AFP and PIVKA-II producing gastric cancer with liver metastasis. Because many cases of this disease have liver metastases at presentation with confusing images with HCC, the diagnosis of liver tumors should be carefully differentiated in the gastric cancer patients with liver tumors, high serum AFP and PIVKA-II levels.

  15. Passage of bone-marrow-derived liver stem cells in a proliferating culture system

    Institute of Scientific and Technical Information of China (English)

    Yun-Feng Cai; Ji-Sheng Chen; Shu-Ying Su; Zuo-Jun Zhen; Huan-Wei Chen

    2009-01-01

    AIM: To explore the feasibility of passage of bonemarrow-derived liver stem cells (BDLSCs) in culture systems that contain cholestatic serum. METHODS: Whole bone marrow cells of rats were purified with conditioning selection media that contained 50 mL/L cholestatic serum. The selected BDLSCs were grown in a proliferating culture system and a differentiating culture system. The culture systems contained factors that stimulated the proliferation and differentiation of BDLSCs. Each passage of the proliferated stem cells was subjected to flow cytometry to detect stem cell markers. The morphology and phenotypic markers of BDLSCs were characterized using immunohistochemistry, reverse transcription polymerase chain reaction (RT-PCR) and electron microscopy. The metabolic functions of differentiated cells were also determined by glycogen staining and urea assay. RESULTS: The conditioning selection medium isolated BDLSCs directly from cultured bone marrow cells. The selected BDLSCs could be proliferated for six passages and maintained stable markers in our proliferating system. When the culture system was changed to a differentiating system, hepatocyte-like colony-forming units (H-CFUs) were formed. H-CFUs expressed markers of embryonic hepatocytes (alpha-fetoprotein, albumin and cytokeratin 8/18), biliary cells (cytokeratin 19), hepatocyte functional proteins (transthyretin and cytochrome P450-2b1), and hepatocyte nuclear factors 1α and -3β). They also had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: BDLSCs can be selected directly from bone marrow cells, and pure BDLSCs can be proliferated for six passages. The differentiated cells have hepatocyte-like phenotypes and functions. BDLSCs represent a new method to provide a readily available alternate source of cells for clinical hepatocyte therapy.

  16. Evaluación de la Autorización de Planes de Precio por Permanencia a las AFP

    OpenAIRE

    Jorge Tarziján M

    2005-01-01

    Debido al débil nivel de competencia en precios que ha mostrado el mercado de AFP en Chile en los últimos años, ha surgido un debate acerca de cómo mejorar este aspecto del mercado. Una idea que ha salido a la discusión es la de promover cambios en las regulaciones a la estructura tarifaria de las AFP que permitan la realización de descuentos por permanencia. Este artículo analiza los posibles efectos sobre la competitividad del mercado de AFP del establecimiento de “premios por permanencia c...

  17. 甲胎蛋白异质体(AFP-L3)检测的临床应用

    Institute of Scientific and Technical Information of China (English)

    巩丽颖; 李光; 卢燕平

    2013-01-01

    根据亲和力不同,AFP被分为三类:AFP-L1、AFP-L2和 AFP-L3。 AFP-L3为肝癌细胞所特有,其占总AFP含量的比值(AFP-L3%)不但可以作为检测原发性肝细胞癌(HCC)的恶性检测指标,也可以作为肝癌手术疗效的评价指标,其临床应用较总AFP准确率高。

  18. STS-39 AFP-675 CIRRIS-1A in OV-103's payload bay (PLB)

    Science.gov (United States)

    1991-01-01

    STS-39's Air Force Program 675 (AFP-675) Uniformly Redundant Array (URA) and Cryogenic Infrared Radiance Instrumentation for Shuttle 1A (CIRRIS-1A) mounted on the experiment support system (ESS) pallet are documented in Discovery's, Orbiter Vehicle (OV) 103's, payload bay (PLB). Part of the ESS pallet power distribution system thermal covering is visible at the bottom of the frame. AFP-675 is a Department of Defense (DOD)-sponsored collection of experiments whose objective is to gather data on the Earth's atmosphere (aurora, Earth limb, and airglow), celestial objects, and the environment in and around the PLB. In the background are the aft PLB bulkhead, the vertical tail, and the orbital maneuvering system (OMS) pods.

  19. STS-39 AFP-675 and STP-1 MPESS in OV-103's payload bay (PLB)

    Science.gov (United States)

    1991-01-01

    An overview of Discovery's, Orbiter Vehicle (OV) 103's, aft payload bay (PLB) documents a variety of STS-39's payloads. In the foreground is the Space Test Payload 1 (STP-1) multipurpose experiment support structure (MPESS) with the Spacecraft Kinetic Infrared Test (SKIRT) (left), ascent particle monitor (APM) (center), and advanced liquid feed experiment (ALFE) (two canisters) visible. Behind the STP-1 is the Air Force Program 675 (AFP-675) experiment support structure (ESS) with ESS tape recorders (left), Uniformly Redundant Array (URA) (front),and Cryogenic Infrared Radiance Instrumentation for Shuttle 1A (CIRRIS-1A) (center back) visible. The ESS pallet power distribution system thermal covering (gold-colored) is visible at the bottom. AFP-675 is a Department of Defense (DOD)-sponsored collection of experiments whose objective is to gather data on the Earth's atmosphere (aurora, Earth limb, and airglow), celestial objects, and the environment in and around the PLB. In the backgroun

  20. Finding the distance between the Roman Pot floor and the AFP Silicon detectors

    CERN Document Server

    Suh, Han Gyeol; Trzebinski, Maciej Marek; CERN. Geneva. EP Department

    2016-01-01

    The ATLAS Forward Proton (AFP) detectors are designed for tagging of forward protons and located inside Roman Pots (RP). My task as summer student is a data analysis to measure the distance between Silicon detectors and the RP floor. For this task there are three suggested methods: tracks pointing to the floor, slope range, and shadow of station. I tried first two methods with data from AFP run 30539. The result from tracking method is 0.7mm, however the unexpected result has to be explained to fully trust this result. In the result of the slope range method, there is an impossible result. Therefore, my code for range method needs debugging in future work.

  1. Measurement and AFPS control of molten steel level in strip casting

    Institute of Scientific and Technical Information of China (English)

    Liye Zhu; Tihua Wu; Yuan Fang

    2005-01-01

    The measurement and control of the molten steel level are studied, which affect the quality of strip surface in strip casting.A system of molten steel measurement with a CCD (Charge Coupled Devices) sensor is designed, real-time measured data are given and its precision is analyzed. The level fluctuation model is derived, and an adaptive fuzzy-PID controller with supervisory control (AFPS) is proposed. The stability of the system is proved using Lyapunov theorem, and the simulation results are given when the model, the casting speed and the roll gap change. It is suggested that this kind of coupled nonlinear and time varying system is stable and robust using the designed AFPS controller.

  2. Sensibilidad de la Demanda con Consumidores Desinformados: El Caso de las AFP en Chile

    OpenAIRE

    Solange Berstein; José Luis Ruiz V

    2005-01-01

    Este artículo analiza la sensibilidad de la demanda previsional con respecto a las variables relevantes, como uno de los elementos fundamentales que ha marcado el comportamiento de la industria de AFP en Chile. Es decir, se analizará el impacto que pueden tener las diferencias en comisiones cobradas y rentabilidades obtenidas por los Fondos de Pensiones en las decisiones de cambiarse o permanecer en una administradora. Cabe destacar que un aspecto distintivo del producto previsional es su obl...

  3. Forming Simulation of Thick AFP Laminates and Comparison with Live CT Imaging

    Science.gov (United States)

    Leutz, Daniel; Vermilyea, Mark; Bel, Sylvain; Hinterhölzl, Roland

    2016-02-01

    Automated fiber placement (AFP) process can be used to manufacture laminates by laying up unidirectional slit tapes along a desired path and placing multiple layers on top of each other. Usually, the slit tapes are placed direct onto the tooling to attain the final part geometry. Alternatively, the laminate can be built up on a planar substrate and can be subsequently formed into the final shape. This kind of processing allows manufacturing highly curved parts, which may not be possible with the direct placement. In the present work a forming simulation of thick AFP laminates is developed to predict the tapes' orientations and delamination as well as transverse tape spread-ups and separations during the forming process. The simulation model is built up through the material characterization experiments. Validation is performed comparing the results of the simulation vs. the experimental forming on two generic geometries. An optical inspection is made on the external layers of the laminates. In a second step, live computer tomography (CT) scans are used to inspect the tapes within an AFP laminate during forming of an L- and a Z-flange. Tapes re-orientation, gaps and tapes widening are observed experimentally and compared to the simulation results. The simulation is capable to predict the tows orientation and provides indicators concerning the tows spread-up and separation.

  4. Combined use of chemotherapeutics and oncolytic adenovirus in treatment of AFP-expressing hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Chen-Yu Mao; Han-Ju Hua; Ping Chen; De-Chao Yu; Jiang Cao; Li-Song Teng

    2009-01-01

    BACKGROUND: Hepatocellular carcinoma (HCC) which is always refractory to most chemotherapeutic agents may result in poor survival of patients with advanced HCC. Oncolytic adenovirus is a new form for cancer gene therapy via its ability to replicate and kill tumor cells in a tumor-speciifc manner. In order to eradicate tumors effectively, the combination of chemotherapeutic agents and oncolytic adenovirus has been considered. This study aimed to systematically analyze the possibility of synergistic cytotoxicity of oncolytic adenoviruses in combination with chemotherapeutic agents. METHODS: Several types of human HCC cell lines were used to determine the speciifcity and cytotoxicity of oncolytic adenovirus Ad5-HC and Ad5-AFP (IRES) by measuring cell viabilityin vitro and antitumor efifciency in vivo. The cytotoxicity of Ad5-HC and Ad5-AFP (IRES) combined with chemotherapeutic agents were also assessed by the methyl thiazolyl tetrazolium assay. RESULTS: Both Ad5-HC and Ad5-AFP (IRES) were signiifcantly cytotoxic to HCC cells with great speciifcity in vitro andin vivo. The combination of oncolytic adenovirus with 5-FU, doxorubicin, and paclitaxel was synergistically effective for the killing of HCC cells. CONCLUSIONS: These data suggest that oncolytic adenovirus sensitize tumors to chemotherapy and the combination therapy of chemotherapeutic agents and oncolytic adenovirus has an enhanced antitumor effect on HCC cells.

  5. Forming Simulation of Thick AFP Laminates and Comparison with Live CT Imaging

    Science.gov (United States)

    Leutz, Daniel; Vermilyea, Mark; Bel, Sylvain; Hinterhölzl, Roland

    2016-08-01

    Automated fiber placement (AFP) process can be used to manufacture laminates by laying up unidirectional slit tapes along a desired path and placing multiple layers on top of each other. Usually, the slit tapes are placed direct onto the tooling to attain the final part geometry. Alternatively, the laminate can be built up on a planar substrate and can be subsequently formed into the final shape. This kind of processing allows manufacturing highly curved parts, which may not be possible with the direct placement. In the present work a forming simulation of thick AFP laminates is developed to predict the tapes' orientations and delamination as well as transverse tape spread-ups and separations during the forming process. The simulation model is built up through the material characterization experiments. Validation is performed comparing the results of the simulation vs. the experimental forming on two generic geometries. An optical inspection is made on the external layers of the laminates. In a second step, live computer tomography (CT) scans are used to inspect the tapes within an AFP laminate during forming of an L- and a Z-flange. Tapes re-orientation, gaps and tapes widening are observed experimentally and compared to the simulation results. The simulation is capable to predict the tows orientation and provides indicators concerning the tows spread-up and separation.

  6. [Demonstration of PIVKA-II and AFP production by gastric cancer by indirect immunoenzymatic staining of paraffin sections].

    Science.gov (United States)

    Nakamura, K; Sakizono, K; Oita, T; Kudo, M; Shirane, H; Uchida, H; Kasakura, S

    1991-06-01

    The patient had elevated plasma PIVKA-II and serum AFP levels. However, no tumor was detected with an ultrasonography, angiography, abdominal computed tomography (CT), and magnetic resonance imaging (MR). Gastric endoscopic examination disclosed gastric cancer and then subtotal gastrectomy was done. Shortly after the surgery, both plasma PIVKA-II and serum AFP levels returned to each normal level. The extirpated tumor revealed histologically immature and mature cancer cells. It was pathologically diagnosed as IIc early cancer. The localization of PIVKA-II and AFP in gastric cancer cells was demonstrated by the immunostaining method using monoclonal antibody. Taken together, these results indicate that cancer cells may produce PIVKA-II or AFP in this patient.

  7. Analysis of relationship between serum AFP,AFP-L3 expression levels and CT images in primary hepatic carcinoma patients%原发性肝癌血清 AFP、AFP-L3表达与 CT 影像的关系

    Institute of Scientific and Technical Information of China (English)

    郭娟; 马刚

    2016-01-01

    目的:探讨原发性肝癌患者血清甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)与 CT 征象的相关性。方法选择2012年2月至2015年3月本院收治的64例原发性肝癌患者作为研究对象,均经手术切除且病理证实,另外选取医院同期确诊的肝硬化患者60例,患者均行多层 CT 常规与增强扫描,ELISA 法测定血清 AFP、AFP-L3,分析 CT 扫描联合 AFP、AFP-L3在原发性肝癌诊断中价值,AFP、AFP-L3表达水平与 CT 征象的相关性。结果 AFP、AFP-L3阳性表达在包膜不完整情况下明显高于包膜完整情况(P <0.05);肿瘤临床分期越晚 AFP、AFP-L3阳性表达率越高(P <0.05),肝细胞癌中 AFP、AFP-L3阳性表达率高于胆管细胞癌(P <0.05),有转移患者AFP、AFP-L3阳性表达率高于非转移者(P <0.05)。结论检测血清 AFP、AFP-L3联合 CT 扫描在诊断原发性肝癌方面有重要意义,血清 AFP、AFP-L3表达与原发性肝癌 CT 影像中肿瘤大小及包膜情况、肿瘤临床分期、病理类型、转移有一定相关性。%Objective This paper was to investigate the relationship between serum AFP,AFP-L3 expression levels and CT images in primary hepatic carcinoma patients.Methods A total of 64 patients with primary liver cancer from February 2012 to March 2015 in hospital were selected,and they were confirmed by surgery and pathology.A total of 60 patients with cirrhosis in the same period were selected.The patients were underwent multisided CT con-ventional and enhanced scan,serum AFP,AFP-L3 was detected by ELISA assay,the value of CT scans combined with AFP,AFP-L3 in the diagnosis of primary liver cancer was analyzed.The relationship between serum AFP, AFP-L3 expression levels and CT images were analyzed.Results The positive expression of AFP-L3 and AFP was significantly higher than that in the whole envelope (P <0.05).The positive expression rates of AFP,AFP-L3 in later clinical

  8. WIRELESS SENSOR NETWORK (WSN) BASED AUTOMATIC FIRING PRACTICE SYSTEM (AFPS) FOR TRAINING OF LAW ENFORCEMENT AGENCIES (LEAS)

    OpenAIRE

    Imran Jattala; Junaid Farooqi; Shakeel Durrani; Nassar Ikram; Irfan Hussian; Muazzam A. Goraya; Obaid B. Zakria

    2014-01-01

    The critical importance of an efficient infantryman in special operations force, tactical paramilitary and Law Enforcement Agencies (LEAs) is insurmountable. One of the many vital aspects of an effective solider is excellent marksmanship which requires extensive training at sophisticated firing ranges. Modern firing ranges are supported by Automatic Firing Practice Systems (AFPS) and this paper presents the design and development of such a system based on WSN. AFPS provide an automatic bullet...

  9. AFP-L3和PIVKA-Ⅱ在肝细胞癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    赵红霞; 郑国军; 史琼英; 樊燕; 王永忠

    2013-01-01

    目的:探讨AFP-L3和PIVKA-Ⅱ在原发性肝癌(HCC)诊断中的价值。方法:应用微量离心柱法分离检测小扁豆凝集素结合型AFP_L3百分含量(AFP-L3%),PIVKA-Ⅱ用电化学发光免疫分析试剂盒检测。结果:肿瘤标志物的判断标准为:AFP-L3%≥10%为阳性,AFP>10ng/ml为阳性,PIVKA-Ⅱ>40mAU/ml为阳性。在慢性肝病组中,AFP》20ng/mL占10.2%,HCC组中则占64.8%。AFP、AFP-L3和PIVKA-Ⅱ在HCC组都明显高于慢性肝病组,差异存在统计学意义(P<0.001)。结论:联合测定AFP-L3和PIVKA-Ⅱ可有效提高HCC的诊断价值。

  10. Growth inhibition and stability of PgAFP from Penicillium chrysogenum against fungi common on dry-ripened meat products.

    Science.gov (United States)

    Delgado, Josué; Acosta, Raquel; Rodríguez-Martín, Andrea; Bermúdez, Elena; Núñez, Félix; Asensio, Miguel A

    2015-07-16

    Dry-ripened foods favor the development of a superficial fungal population that may include toxigenic molds. To combat unwanted molds, an antifungal protein from Penicillium chrysogenum (PgAFP) can be useful. The aim of the present work was to study the antimicrobial activity of PgAFP against microorganisms common in dry-ripened foods, and to evaluate its sensitivity to proteolytic enzymes and heat treatments that may be applied to foods, as well as to different pH values. The inhibitory effect of the purified protein on 38 microbial strains grown in culture medium was determined. PgAFP sensitivity to various proteases, heat treatments, and preincubation at different pH values was tested by means of the residual activity on selected reference strains. Inhibitory activity of PgAFP against unwanted molds was tested in a dry-fermented sausage. This protein exhibited potent inhibitory activity against unwanted molds, including the main mycotoxin-producing species of Aspergillus and Penicillium of concern for dry-ripened foods. PgAFP withstood most proteases, intense heat and a wide range of pH values. PgAFP efficiently reduced counts of A. flavus and P. restrictum inoculated on a dry-fermented sausage. This protein can be of interest to control hazardous molds in dry-ripened foods. PMID:25874751

  11. Expression and differential diagnostic value of serum AFP,AFP-L3% and GP73 in patients with liver cirrhosis%AFP、AFP-L3%、GP73在肝硬化、肝癌患者血清中的表达及鉴别诊断价值

    Institute of Scientific and Technical Information of China (English)

    王松; 袁春蓓; 俞海英; 潘剑; 杨永峰

    2014-01-01

    目的:探讨不同血清肿瘤标志物在肝硬化和肝癌的鉴别诊断中的临床价值。方法回顾性分析146例肝硬化患者、50例原发性肝癌患者的临床资料,并选取50例健康体检者作为对照,分别检测血清AFP、甲胎蛋白异质体(AFP-L3)和高尔基体糖蛋白73(GP73)含量,并计算 AFP-L3%。结果146例肝硬化患者的 AFP 阳性率为平均25.3%,低于肝癌组的72%(χ2=34.69,P<0.01);AFP-L3%在不同病因肝硬化组中阳性率均较低,平均8.9%,低于肝癌组的58%(χ2=53.32,P<0.01),也低于肝硬化组中AFP 的阳性率(χ2=13.90,P=0.0002);GP73在不同病因肝硬化组、肝癌组阳性率均较高,在肝硬化组中平均阳性率为74.7%,高于AFPAFP-L3%在肝硬化组中的阳性率(χ2=71.01,P<0.01),与GP73在肝癌组中的阳性率比较差异无统计学意义(χ2=0.84,P=0.36)。AFP、AFP-L3%的敏感性、特异性均较GP73好,联合诊断不能提高鉴别准确性。结论血清肿瘤标志物AFP 在乙型肝炎肝硬化及隐源性肝硬化患者中阳性率较高,AFP-L3%在各种病因肝硬化患者中均较低,AFP、AFP-L3%可有效区分肝癌和肝硬化;GP73在不同病因肝硬化组中均高,不能用于区分肝硬化和肝癌。%Objective To investigate the difference of serum AFP,AFP-L3% and GP73in different types of liver cirrhosis and their differential diagnostic value between liver cirrhosis and liver cancer. Methods One hundred and forty-six cases of liver cirrhosis patients from August 2012 to August 2013 were analyzed,while 50 primary hepatic carcinoma (PHC)and 50 healthy controls were enrolled. Serum AFP,AFP-L3 and GP73 were measured respectively and AFP-L3% was calculated. Results The top three causes of liver cirrhosis were hepatitis B virus, cryptogenic and autoimmunity. The positive rate of AFP in hepatitis virus groups and

  12. AFP、AFP-L3、GP73联合检测对原发性肝癌的诊断价值%Application of Combined Detection of AFP,AFP-L3,GP73 in the Diagnosis of Primary Hepatocellular Cancer

    Institute of Scientific and Technical Information of China (English)

    赵树艳

    2015-01-01

    目的:探讨甲胎蛋白(AFP)、甲胎蛋白异质体-L3(AFP-L3)、高尔基体蛋白73(GP73)在原发性肝癌的诊断价值。方法采用酶联免疫吸附法和电化学发光法检测68例原发性肝癌、49例良性肝病及68例健康体检者血清中 AFP、AFP-L3、GP73的含量,并进行比较。结果原发性肝癌患者组血清 AFP、AFP-L3、GP73含量明显高于良性肝病及健康对照组(P <0.05和 P <0.01);三者联合检测的特异度和敏感度优于单独检测,所得数据有统计学意义。结论AFP、AFP-L3、GP73联合检测可明显提高原发性肝癌诊断的敏感性和特异性。%Objective To investigate the application value of detection AFP,AFP-L3 and GP73 in the diagnosis of hepatocellular carcinoma.Methods Enzyme-linked immunosorbent assay (ELISA)was adopted to detect AFP、AFP-L3 and GP73 level of senum in hepatocellular carcinoma patients(68cases),benign liver disease (49 cases)and healthy people (68cases),and comparison was done.Results the Serum AFP,AFP-L3 and GP73 level in hepatocellular carci-noma were significantly higher than those of patients with benign liver diseases and control group (P < 0.05,P <0.01).The specificity and sensitivity of the combined detection of the three was better than that of the individual test, and the difference was statistically significant.Conclusion Combination test of serum AFP,AFP-L3 and GP73 has good diagrnosis value for hepatocellular carcinoma.

  13. [Tumor markers for hepatocellular carcinoma].

    Science.gov (United States)

    Tateishi, Ryosuke; Enooku, Kenichiro; Shiina, Shuichiro; Koike, Kazuhiko

    2012-05-01

    Three tumor markers for hepatocellular carcinoma (HCC) are available in Japan: alpha-fetoprotein (AFP), protein induced by vitamin K absence or antagonists-II (PIVKA-II), and Lens culinaris agglutinin-reactive fraction of alpha-fetoprotein (AFP-L3). Although AFP has drawbacks in its specificity, it is widely utilized in treatment evaluation and prognosis prediction. PIVKA-II is a unique marker that does not correlate with AFP value and can predict microvascular invasion. AFP-L3 is a highly specific marker and strong predictor of poor prognosis. These three markers are indispensable in every aspect of clinical practice of hepatocellular carcinoma including surveillance, diagnosis, treatment evaluation, and prognosis prediction.

  14. 血清AFP、AFP-L3及GP73三者联合检测在原发性肝癌诊断中的应用探究%Diagnostic Value of The Combination With Detection of AFP, AFP-L3 and GP73 in Primary Liver Cancer

    Institute of Scientific and Technical Information of China (English)

    刘明浩; 姚丽

    2016-01-01

    Objective To investigate and analyze the application value of combined detection of serum alpha (AFP), alpha fetal protein (AFP-L3) and Golgi protein (GP73) in the diagnosis of primary hepatic cancer.Methods We Selected 40 cases of HCC patients (group A), 40 cases of benign liver disease patients(group B)and 40 cases of healthy volunteers (group C) as the research object. The serum levels of AFP, AFP-L3 and GP73 were detected by electrochemical luminescence, micro column and enzyme linked immunosorbent assay (ELISA). Results AFP, AFP-L3, GP73 were not detected in the serum of patients with C group.But group A and B were signiifcantly higher than those in the control group, and the difference was statistically significant (P<0.05). The positive rates of AFP-L3, GP73 in group A were 70%and 77.5%. Compared with the positive rate of AFP serum detection, the difference was statistically signiifcant (P<0.05). But significantly lower than the positive rate of AFP,AFP-L3 and GP73,the difference was statistically significant (P<0.05). The sensitivity and specificity of the combined detection of AFP,AFP-L3 and GP73 were 96.4%,88.4%,they were better than those of the above indexes,the difference was statistically signiifcant (P<0.05). Conclusion The combined detection of AFP,AFP-L3 and GP73 had important significance in the diagnosis of primary hepatic cancer, which could signiifcantly improve the patient positive test results, while ensure a better sensitivity and speciifcity.%目的:探讨和分析血清甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)、高尔基体蛋白(GP73)三者联合检测在原发性肝癌诊断中的应用价值。方法选取40例肝癌患者(A组)、40例肝脏良性疾病患者(B组)和40例健康志愿者(C组)作为此次研究的对象,分别采用电化学发光法、微量离心柱法和酶联免疫吸附法(ELISA)对上述对象血清中AFP、AFP-L3及GP73进行检测。结果 C组血清均未检出AFP、AFP

  15. Expression, purification and activity determination of the beetle tenebrio molitor antifreeze protein afp84c in escherichia coli

    International Nuclear Information System (INIS)

    Summary: A cDNA encoding antifreeze protein (AFP84c) was cloned by RT-PCR from the larva of the yellow mealworm Tenebrio molitor. The coding fragment of 252 bp encodes a protein of 84 amino acid residues and was fused to the expression vectors pMAL-c2X and pMAL-p2X. The expression plasmids pMAL-c2X-afp84c and pMAL-p2X-afp84c were constructed and transformed into Escherischia coli strains TBI, respectively. Strategy of optimization of induction conditions were used for expression of the highly disulfide-bonded beta-helix-contained protein with the activity of antifreeze in pMALTM expression system. The target fusion protein was released from the cytoplasm and periplasm by sonication and cold osmotic shock procedure respectively. Recombinant AFP84c was purified by amylose affinity column. The purified target protein displayed a single band in SDS-PAGE. Expressed AFP84c exhibits to increase low temperature resistance of bacteria. (author)

  16. AFP-L3和AFP mRNA联合检测在肝癌治疗疗效观察和预后中的作用

    Institute of Scientific and Technical Information of China (English)

    邱大鹏; 韩风; 聂常富; 周进学; 蒙博

    2014-01-01

    目的:探讨甲胎蛋白(AFP)异质体(AFP-L3)和AFP mRNA联合检测在肝癌(HCC)治疗疗效观察和预后中的作用。方法联合检测AFP-L3、AFPmRNA在HCC患者中表达情况。结果 HCC患者的AFP mRNA(368.65±5.28)和AFP-L3(195.84±3.31)蛋白表达量均明显高于肝硬化组和肝良性病变组(t=23.32、30.15、30.21、25.46,均P<0.01)。另外,HCC患者的AFP阳性率(69.35%)和AFP-L3阳性率(79.03%)明显高于肝硬化组和肝良性病变组(χ2=5.78、10.98、14.52、26.23,均P<0.05)。62例HCC患者中,术后AFP转阴48例,其中 AFP-L3仍为阳性18例,其1、2、3年生存率分别为72.22%(13/18)、44.44%(8/18)、22.22%(4/18);另外30例患者AFP和AFP-L3均为阴性,其1、2、3年生存率分别为80.00%(24/30)、53.33%(16/30)、50.00%(15/30);AFP和AFP-L3均转阴者的3年生存率明显高于AFP转阴而AFP-L3未阴者(χ2=3.98,P<0.05)。结论:测定患者AFP mRNA 和AFP-L3蛋白水平可以鉴定良、恶性肝病,对于HCC 的早期诊断及预后判断有重要的临床意义。

  17. Identification of Serum Monocyte Chemoattractant Protein-1 and Prolactin as Potential Tumor Markers in Hepatocellular Carcinoma

    NARCIS (Netherlands)

    Wang, Who-Whong; Ang, Soo Fan; Kumar, Rajneesh; Heah, Charmain; Utama, Andi; Tania, Navessa Padma; Li, Huihua; Tan, Sze Huey; Poo, Desmond; Choo, Su Pin; Chow, Wan Cheng; Tan, Chee Kiat; Toh, Han Chong

    2013-01-01

    Early diagnosis of hepatocellullar carcinoma (HCC) remains a challenge. The current practice of serum alpha-fetoprotein (AFP) measurement is inadequate. Here we utilized a proteomic approach to identify novel serum biomarkers for distinguishing HCC patients from non-cancer controls. We profiled the

  18. LHC physics debris collimation studies and their impact on AFP detectors acceptance

    CERN Document Server

    Roncarolo, F; Potter, K; Bussey, P; Bracco, C

    2011-01-01

    The ATLAS Forward Proton (AFP) group is proposing to upgrade the forward region of ATLAS by installing forward proton detectors at 220 m and 420 m from the interaction point on both sides of the LHC ATLAS experiment. For this purpose, at both the 420 m and 220 m locations, it is proposed to install movable beam pipes which will host silicon tracking and fast timing detectors (i.e. four independent detector stations). The experimental acceptance at 220 m is dependent upon the setting of two collimators designed to protect the LHC straight section and dispersion suppressor around ATLAS (and CMS) from the physics debris generated at the two high luminosity experiments. This note presents the result of tracking studies showing that the installation of a new collimator in front of the Q6 magnet (or the displacement at this of location of the second of the already existing collimators) would ensure an improved protection of the LHC machine while allowing the AFP experiment at 220m.

  19. Marginal microleakage of class V composite restorations before and after AFP gel application

    Directory of Open Access Journals (Sweden)

    Davari Abdolrahim

    2015-01-01

    Full Text Available   Background and Aims: The most effective preventing tooth decay method is fluoride compounds applications. Some studies suggested that APF gels caused changes on the superficial physical properties of composite. Therefore, the purpose of this study was to evaluate the marginal microleakage of class V composite restorations before and after AFP gel application.   Materials and Methods: The class V cavities in buccal surfaces of 45 molar teeth were made in such a way that occlusal margin was placed in enamel and cervical margin in cement. In group 1, at first fluoride-therapy and then cavity preparation and restoration by composite resin was done. In group 2, at first the class V cavities were prepared and restored, then fluoride-therapy was carried out. In group 3, cavities were prepared and restored with no fluoride-therapy. The dye penetration rate in occlusal and cervical margins was examined by stereomicroscope. Data were statistically analyzed using Kruskal-Wallis and Mann-Whitney test.   Results: There was no statistically significant difference between groups ( P=0.975.   Conclusion: Fluoride-therapy using AFP gel before and after class V composite restorations, had no significant effect on the microleakage of dentin and enamel margins.

  20. Mesenchymal stem cells from the human umbilical cord ameliorate fulminant hepatic failure and increase survival in mice

    Institute of Scientific and Technical Information of China (English)

    Jin-Feng Yang; Hong-Cui Cao; Qiao-Ling Pan; Jiong Yu; Jun Li; Lan-Juan Li

    2015-01-01

    BACKGROUND:Cell therapy has been promising for various diseases. We investigated whether transplantation of human umbilical cord mesenchymal stem cells (hUCMSCs) has any therapeutic effects on D-galactosamine/lipopolysaccharide (GalN/LPS)-induced fulminant hepatic failure in mice. METHODS:hUCMSCs isolated from human umbilical cord were cultured and transplanted via the tail vein into severe combined immune deifciency mice with GalN/LPS-induced fulminant hepatic failure. After transplantation, the localiza-tion and differentiation of hUCMSCs in the injured livers were investigated by immunohistochemical and genetic analy-ses. The recovery of the injured livers was evaluated histologi-cally. The survival rate of experimental animals was analyzed by the Kaplan-Meier method and log-rank test. RESULTS:hUCMSCs expressed high levels of CD29, CD73, CD13, CD105 and CD90, but did not express CD31, CD79b, CD133, CD34, and CD45. Cultured hUCMSCs displayed adip-ogenic and osteogenic differentiation potential. Hematoxylin and eosin staining revealed that transplantation of hUCMSCs reduced hepatic necrosis and promoted liver regeneration. Transplantation of hUCMSCs prolonged the survival rate of mice with fulminant hepatic failure. Polymerase chain reaction for humanalu sequences showed the presence of human cells in mouse livers. Positive staining for human albumin, human alpha-fetoprotein and human cytokeratin 18 suggested the for-mation of hUCMSCs-derived hepatocyte-like cellsin vivo. CONCLUSIONS:hUCMSC was a potential candidate for stem cell based therapies. After transplantation, hUCMSCs partially repaired hepatic damage induced by GalN/LPS in mice. hUC-MSCs engrafted into the injured liver and differentiated into hepatocyte-like cells.

  1. Non-polio enteroviruses associated with acute flaccid paralysis (AFP) and facial paralysis (FP) cases in Romania, 2001-2008.

    Science.gov (United States)

    Persu, Ana; Băicuş, Anda; Stavri, Simona; Combiescu, Mariana

    2009-01-01

    Acute flaccid paralysis is a complex clinical syndrome, with a wide variety of possible etiologies and with clinical manifestations that can vary according to age or geographical region. Enteroviruses (polioviruses and non-polio enteroviruses) are among the viral agents that can cause AFP. AFP surveillance is important for public health through its use in monitoring poliomyelitis, in the context of the Global Initiative to eradicate this disease. The current paper aims to assess the non-polio enteroviruses (NPEV) association with AFP and FP cases registered in Romania in the period 2001-2008 and to identify prevalent serotypes. Within the framework of Surveillance of AFP Cases Program, were collected samples from 579 children with AFP or FP (3.069 samples). The samples were processed and inoculated onto two types of cell culture (RD and L20B), according to WHO protocol. The identification of isolated viruses has been done by the reaction of seroneutralization with pools of specific antiserum and then with monospecific antiserum for confirmation. NPEV were isolated from 58 cases (123 positive samples). During the analyzed period, 23 NPEV serotypes have circulated (15 Echo serotypes and 8 coxsackie serotypes). The most frequently identified were the Echoviruses 13 and 11 and the coxsackie A viruses. 88% of positive cases have occurred in children between 1 and 5 years. As seasonal distribution, the peak of NPEV circulation was in the months August-September (36.2%). The paper provides information about NPEV circulation in Romania in the past 8 years, about its association with the AFP and FP and it indicates the need for monitoring NPEV circulation even after the eradication of poliomyelitis.

  2. Role of blood AFP mRNA and tumor grade in the preoperative prognostic evaluation of patients with hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Umberto Cillo; Giacomo Zanus; Vito De Pascale; Mario Plebani; Davide Francesco D'Amico; Alessandro Vitale; Filippo Navaglia; Daniela Basso; Umberto Montin; Marco Bassanello; Francesco D'Amico; Francesco Antonio Ciarleglio; Alberto Brolese

    2005-01-01

    AIM: To explore the potential prognostic role of preoperative tumor grade and blood AFP mRNA in a cohort of patients with hepatocellular carcinoma (HCC)eligible for radical therapies according to a well-defined treatment algorithm not including nodule size and number as absolute selection criteria.METHODS: Fifty patients with a diagnosis of HCC were prospectively enrolled in the study. Inclusion criteria were: (1) histological assessment of tumor grade by means of percutaneous biopsies; (2) determination of AFP mRNA status in the blood; (3) patient's eligibility for radical therapies.RESULTS: At preoperative evaluation, 54% of the study group had a well-differentiated HCC, 42% had AFP mRNA in the blood, 40% had a tumor larger than 5 cm and 56% had more than one nodule. Surgery (resection or liver transplantation) was performed in 29 patients,while 21 had percutaneous ablation procedures. After a median follow-up of 28 mo, 12-, 24-, and 36-mo survival rates were 78%, 58%, and 51%, respectively. Surgical therapy, performance status and three tumor-related variables (AFP mRNA, HCC grade and gross vascular invasion) resulted as significant survival predictors at univariate analysis. Nodule size and number did not perform as significant prognosticators. Multivariate study selected only surgical therapy and a biologically early HCC profile (AFP mRNA negative and well-differentiated tumor without gross vascular invasion) as independent survival variables.CONCLUSION: The preoperative determination of tumor grade and blood AFP mRNA status may potentially refine the prognostic evaluation of HCC patients and improve the selection process for radical therapies.

  3. Stem-loop oligonucleotide beacons as switches for amplifying-fluorescent-polymer-based biological warfare sensors

    Science.gov (United States)

    Clinkenbeard, Kenneth D.; Ramachandran, Akhilesh; Malayer, Jerry R.; Moon, Joong Ho; Hancock, Lawrence F.

    2003-09-01

    Sensors that are exceptionally sensitive with real-time outputs and minimal consumption of reagents are needed to continuously monitor air and water against bioterrorist incidents. Amplifying fluorescent polymers (AFP) provide exceptionally sensitive real-time reagentless sensor platforms as applied to detection of nitroaromatic explosives. This platform technology has the potential to be adapted to detect biological warfare (BW) agents by covalently attaching the 5" end of stem-loop molecular beacons to AFP as DNA hybridization signal transduction switches. Molecular beacons with loop sequences specific for sequence signatures of a target BW agent are configured with a quencher on the end of the 3" arm of the stem-loop. The AFP is quenched in the absence of target DNA, but upon hybridization with target the stem is melted, the duplex loop extended, and the AFP dequenched. This signal transduction is reversible upon removal of the target sequence with the molecular beacon reforming the stem-loop conformation. Proof-of-concept research has demonstrated that molecular beacons for signature sequences of Francisella tularensis result in correct identification of the presence of this agent in samples, but no false positives were seen with Escherichia coli.

  4. Analysis of Significance of Unite Examination of AFP and DNA Polymorphism of P3 Promoter of IGF-ⅡGene

    Institute of Scientific and Technical Information of China (English)

    LUO Su; ZHANG Feng-chun; SUN Chang-jiang; LIU Cheng-bai; ZHUANG Jiang-xing; ZHANG Jin

    2004-01-01

    The DNA of P3 promoter region of IGF-Ⅱ gene was obtained by means of PCR technique. The examination of DNA polymorphism by restriction endonuclease BstE Ⅱ and the examination of AFP by bioluminescence immunoassay technique were carried out. The results have a significant difference(P<0.005). But the positive rate of AFP is higher than that of DNA polymorphism. The experimental result shows that the change of the DNA polymorphism of IGF-Ⅱis not the only carcinogenic factor. The suggested unite examination is the best method for the diagnosis of the primary hepatocellular carcinoma.

  5. The expressions of serum tumor biomakers DCP,AFP-L3 and AFP in patients with hepatocellular carcinoma and its clinical significance%肝细胞癌血清肿瘤标志物DCP、AFP-L3和AFP的表达及临床意义

    Institute of Scientific and Technical Information of China (English)

    刘传苗; 徐静

    2012-01-01

    目的:分析肝细胞癌(HCC)的3种肿瘤标志物脱-γ-异常凝血酶原(DCP)、甲胎蛋白异质体(AFP-L3)和甲胎蛋白(AFP)单独或联合检测,诊断HCC的临床意义.方法:分别用酶联免疫吸附试验检测HCC组、肝硬化组和慢性乙型肝炎(慢乙肝)组患者血清DCP、AFP-L3%和AFP水平,并比较3组患者肿瘤标志物水平的差异;利用诊断试验评价分析3种肿瘤标志物单独或联合检测诊断HCC的敏感性、特异性、曲线下面积及Youden指数.结果:HCC组患者血清DCP、AFP-L3%和AFP水平均高于肝硬化和慢乙肝组患者(P<0.01).在40 mAU/ml的临界值下,DCP诊断HCC的敏感性和特异性为87.88%和95.00%;在10%的临界值下,AFP-L3%诊断HCC的敏感性和特异性分别为57.58%和90.00%;在20 ng/ml的临界值下,AFP诊断HCC的敏感性和特异性分别为75.76%和63.33%;2种或3种标志物联合检测诊断HCC的敏感性和特异性均未高于DCP单独检测.结论:无论是DCP、AFP-L3%和AFP单独检测还是联合检测,DCP均具有最好的敏感性和特异性,或许可以作为发现HCC的主要血清标志物.%Objective: To analyze the clinical significance of detection of des-γ-carboxyprohrombin ( DCP) , α-fetoprotein-L3 ( AFP-L3% ) and α-fetoprotein(AFP) alone or in combination for diagnosis of hepatocellular carcinoma( HCC). Methods: The serum levels of those three biomarkers were detected by ELISA in patients with HCC, cirrhosis and chronic hepatis B and compared with each other. The diagnostic evaluation of the three tumor biomarkers alone or combined detection was used to analyze the sensitivity, specificity, the area under the curve and Youden index in the diagnosis of HCC. Results:The value of the three biomarkers in HCC patients were higher than those in cirrhosis and chronic hepatis B patients ( P < 0. 01) . In the cut-off value of 40 mAU/ml, 10% and 20 ng/ml for DCP, AFP-L3% and AFP,the sensitivity and specificity were 87. 88% and 95. 00% ,57. 58% and 90. 00

  6. A new mutation in the AFP gene responsible for a total absence of alpha feto-protein on second trimester maternal serum screening for Down syndrome

    Science.gov (United States)

    Petit, François M; Hébert, Marylise; Picone, Olivier; Brisset, Sophie; Maurin, Marie-Laure; Parisot, Frédéric; Capel, Liliane; Benattar, Clarisse; Sénat, Marie-Victoire; Tachdjian, Gérard; Labrune, Philippe

    2009-01-01

    Alpha feto-protein (AFP) is a major plasma protein produced by the yolk sac and the liver during the fetal period. During the second trimester of pregnancy, APF and βhCG serum concentrations are commonly used for screening Down syndrome. AFP deficiency is rare (estimated to be 1/105 000 newborns) and only one sequence alteration has previously been reported in the AFP gene. We report a new mutation in exon 5 of the AFP gene, leading to a total absence of AFP on 2nd-trimester maternal serum screening for Down syndrome, confirmed on the amniotic fluid. Despite this, fetal development and birth were normal. After PCR-amplification, the whole AFP gene was sequenced. The new mutation was a guanine to adenine transition in position 543 creating a premature stop codon in position 181. In order to search for eventual modifications of the amniotic fluid profile, proteins were separated by electrophoresis and compared with 10 normal amniotic fluids sampled at the same developmental age (18 weeks). In the amniotic fluid of our patient albumin rate was reduced whereas alpha1 and beta protein fractions were increased, suggesting that AFP deficiency may modify the distribution of protein fractions. This observation emphasizes the complex molecular mechanisms of compensation of serum protein deficiency. Studies on other families with AFP deficiency are necessary to confirm this observation. PMID:18854864

  7. The ATLAS Forward Detectors - LUCID, ALFA and AFP: Past, Present and Future

    CERN Document Server

    Caforio, D; The ATLAS collaboration

    2013-01-01

    LUCID is a gas Cerenkov detector deployed near to the beam‐pipe at 17 m either side of the ATLAS Intersection point. The LUCID detector ‐ when calibrated via a Van der Meer scan – is now measuring absolute luminosity on a bunch‐by‐bunch basis. It is now measuring integrated luminosity up to high luminosity with a precision of around 3%. The technical challenges that had to faced to make this measurement as well as upgrade plans for LUCID will be presented. In addition to LUCID, we will discuss that ALFA detector (Absolute Luminosity for ATLAS) and its status as well as the progress on the ATLAS Forward Protons project (AFP) that plans to deploy detectors to tag and measure, with 3‐D silicon detectors, both protons in exclusive central diffractive processes.

  8. Value of GPC3, GP73, AFP-L3 and AFP detection in the diagnosis of primary hepatic carcinoma%血清GPC3、GP73、AFP-L3和AFP检测对原发性肝癌诊断的价值

    Institute of Scientific and Technical Information of China (English)

    龙璐; 陈贞; 王堃; 陶亚; 易斌

    2013-01-01

    目的 探讨血清磷脂酰肌醇蛋白多糖-3(glypican-3,GPC3)、高尔基体蛋白73(Golgi protein-73,GP73)、甲胎蛋白异质体AFP-L3以及AFP单独及联合检测在原发性肝癌(primary hepatic carcinoma,PHC)中的诊断价值.方法 GPC3、GP73、AFP-L3检测采用ELISA法,AFP检测采用化学发光法,分别检测原发性肝癌组(43例)、肝硬化组(43例)、慢性肝炎组(43例)、和健康对照组(43例)血清GPC3、GP73、AFP-L3、AFP水平,应用SPSS统计软件对结果进行统计学分析.结果 PHC组GP73、AFP-L3和AFP水平高于其他各组,差异均有显著性(P =0.000).GPC3、GP73、AFP-L3和AFP的ROC曲线下面积(AUCROC)分别为0.744、0.910、0.903、0.867,灵敏度分别为53.5%、86.0%、81.4%、76.7%,特异度分别为94.6%、93.8%、95.3%、86.0%.系列试验以GP73+AFP-L3为最佳组合,灵敏度和特异度分别为70.0%和99.7%,平行试验以AFP/AFP-L3/GP73为最佳组合,灵敏度特异度分别为99.4%和76.9%.结论 GP73和AFP-L3有望成为新的诊断原发性肝癌的血清标志物.临床应用中,可用GP73+AFP-L3作为诊断PHC的最佳组合,GP73/AFP-L3/AFP作为排除PHC较好的组合.

  9. 甲胎蛋白及甲胎蛋白异质体L3在肝癌患者肝移植手术前后的变化%The content change of AFP and AFP-L3 pre-and post-liver transplantation operation for liver cancer patients

    Institute of Scientific and Technical Information of China (English)

    谭宗宪; 侯俊; 朱安琪; 李伯安

    2010-01-01

    目的 探讨甲胎蛋白(AFP)及甲胎蛋白异质体L3(AFP-L3)在肝癌患者肝移植手术前后的变化情况.方法 对12例确诊为肝癌并进行肝移植手术患者的连贯血清(术前1份,术后连续留取),应用甲胎蛋白异质体微量离心柱分离并洗脱获得AFP-L3,再同时检测原始血清中AFP及AFP-L3含量,计算AFP-L3在AFP中比例(AFP-L3%),对比手术前后AFP含量及AFP-L3%的变化情况.结果 AFP除5例转阴外,其余患者在AFP-L3%转阴后,AFP仍持续阳性,个别患者AFP含量还持续高水平.2例患者术后AFP-L3立即转阴,其他患者在肝移植术后AFP-L3含量逐步下降,并趋于阴性(AFP-L3%<10%).AFP半衰期与AFP-L3%半衰期之间差异无统计学意义.结论 肝癌经根治性切除或移植后,AFP-L3%一般在较短时间内(约1~20 d)转阴,AFP-L3占AFP百分含量占10%以下,提示手术可能较为彻底,但余肝尚存在肝炎或肝硬化.如AFP-L3含量持续不降低提示手术未彻底清除病灶.%Objective To explore the content change of Alpha Fetal Protein(AFP) and Alpha Fetal Protein Variants(AFP-L3) pre-and post-liver transplantation operation for liver cancer patients.Methods AFP-L3 was de-termined by micro centrifugal column from twelve cases of successive sera(For each case,one original shale pre-oper-ation,and mole shares post-operation successively),and preserved from patients who were diagnosed as fiver cancer and completed fiver transplantation operation.simultaneously the content of AFP and AFP-L3 in the original serum were also determined.and the ratio of AFP-L3 in AFP was calculated in order to compare the content change of AFP and the ratio of AFP-L3 pre-and post-liver transplantation operation.Results Compared with twelve cases of pre-and post-operation,the AFP in only five cases turned tO negative;The AFP in other cases keeped positive though AFP-L3%tumde to negative.and the content of AFP in some specific cases still keeped in a high level.Regarding AFP-L3

  10. Increased chitin biosynthesis contributes to the resistance of Penicillium polonicum against the antifungal protein PgAFP.

    Science.gov (United States)

    Delgado, Josué; Owens, Rebecca A; Doyle, Sean; Asensio, Miguel A; Núñez, Félix

    2016-01-01

    Antifungal proteins from molds have been proposed as a valuable tool against unwanted molds, but the resistance of some fungi limits their use. Resistance to antimicrobial peptides has been suggested to be due to lack of interaction with the mold or to a successful response. The antifungal protein PgAFP produced by Penicillium chrysogenum inhibits the growth of various ascomycetes, but not Penicillium polonicum. To study the basis for resistance to this antifungal protein, localization of PgAFP and metabolic, structural, and morphological changes were investigated in P. polonicum. PgAFP bound the outer layer of P. polonicum but not regenerated chitin, suggesting an interaction with specific molecules. Comparative two-dimensional gel electrophoresis (2D-PAGE) and comparative quantitative proteomics revealed changes in the relative abundance of several proteins from ribosome, spliceosome, metabolic, and biosynthesis of secondary metabolite pathways. The proteome changes and an altered permeability reveal an active reaction of P. polonicum to PgAFP. The successful response of the resistant mold seems to be based on the higher abundance of protein Rho GTPase Rho1 that would lead to the increased chitin deposition via cell wall integrity (CWI) signaling pathway. Thus, combined treatment with chitinases could provide a complementary means to combat resistance to antifungal proteins.

  11. WIRELESS SENSOR NETWORK (WSN BASED AUTOMATIC FIRING PRACTICE SYSTEM (AFPS FOR TRAINING OF LAW ENFORCEMENT AGENCIES (LEAS

    Directory of Open Access Journals (Sweden)

    Imran Jattala

    2014-12-01

    Full Text Available The critical importance of an efficient infantryman in special operations force, tactical paramilitary and Law Enforcement Agencies (LEAs is insurmountable. One of the many vital aspects of an effective solider is excellent marksmanship which requires extensive training at sophisticated firing ranges. Modern firing ranges are supported by Automatic Firing Practice Systems (AFPS and this paper presents the design and development of such a system based on WSN. AFPS provide an automatic bullet-impact count during firing training session and is modular scalable in design for multiple of eight concurrent shooters. The system is versatile and flexible allowing for different small-arms and firing training modes and supports night firing exercise. AFPS comprises of two major components, the automatic target-box and a commander console. Automatic target-box has a motor & gear assembly, target sheet, bullet-impact sensor, control board, and WiFi communication module. Commander console is a ruggedized sunlight readable 10.4” Tablet PC, which with a built-in WiFi acts an access point. The automatic target-boxes equipped with embedded WiFi modules form sensor nodes of a WSN. The paper presents the complete System Development Life Cycle (SDLC of the firing practice system and associated WSN. The AFPS and bullet-impact sensor was extensively tested on Firing Ranges for accuracy of bullet-impact count. The results showed a bullet-impact count accuracy of over 97 percent.

  12. Construction of recombinant adenovirus vector containing AFP and generation of adenovirus-mediated AFP gene modified dendritic cells vaccine%含人AFP基因重组腺病毒载体的构建及其转染树突状细胞瘤苗的制备

    Institute of Scientific and Technical Information of China (English)

    杨静悦; 曹大勇; 刘文超; 斯小明

    2009-01-01

    Objective:To construct recombinant adenovirus vectors containing human AFP genes,and infect dendritic cell. Methods: Full length AFP cDNAs were subcloned into pIND vector,followed by being cloned into shuttle2 vector.The AFP gene fragments resulted from the shuttle2-AFP digested with PI-Sce and I-Ceu were linked to the linear adeno-X virus DNA.After packaged with HEK293 cells,the adenovirus expression vector was obtained.The plasmid pAdeno-AFP was identified by endonuclease and PCR.After dendritic cells were infected pAdeno-AFP,the surface molecules of pAdeno-AFP/DC were analysed by flow cytometry.AFP levels in culture supernatant of pAdeno-AFP/DC were measured by ELISA. Results: AFP gene in the inserted DNA of adeno-AFP was confirmed by PCR,and predictive fragments proved by restriction enzyme digestion analysis were exhibited.All the above results indicated that human AFP gene had been connected with pAdeno-X vectors correctly.The recombinant adenovirus vector of human AFP gene packaged in HEK293 cells,it will be used to introduce the target gene into dendritic cell.pAdeno-AFP/DC were able to upregulate CD1a,CD11c,CD80,CD86 and HLA-DR.And pAdeno-AFP/DC could secrete high level of AFP in vitro. Conclusion: The recombinant adenovirus vector of human AFP gene have been constructed successfully.The established AFP -DC vaccine may be a tool of the hepatocellular carcinoma immunotherapy,and it will be the foundation of future clinical use of DC vaccine.%目的:构建含人AFP基因的腺病毒载体,体外转染树突状细胞,制备树突状细胞肝癌瘤苗.方法: 将AFP基因亚克隆到pIND 载体和Shuttle2载体中,构建穿梭载体Shuttle2-AFP.用PI-Sce Ⅰ和I-CeuⅠ双酶切后将所获AFP基因片段再与线性化的腺病毒载体pAdeno-X连接,构成pAdeno-AFP重组腺病毒载体.其后,用重组腺病毒载体转染HEK293细胞,包装腺病毒表达载体.通过酶切、PCR对腺病毒载体进行鉴定.包装好的重组病毒载体pAdeno-AFP体外

  13. Effect Observations of 28 Cases of Treating AFP Increase Caused by Virus Hepatitis with Cucurbitacin%葫芦素治疗病毒性肝炎致甲胎蛋白升高28例疗效观察

    Institute of Scientific and Technical Information of China (English)

    黄雄立

    2002-01-01

    To treat 28 patients with virus hepatits complicated with AFP in crease by using cucurbitacin 0.6 mg/day. On the basis ofcomprehensive treatment and testing AFP regularly. The result is that 26 cases of the treatment group (28 cases) decreased in AFP with 92.85% total effective rate which is higher than the control group (57.14%). The difference is significant (P < 0.01 ). Conclusioncucurbitacin can decrease AFP effectively.

  14. Performance of Protein Induced by Vitamin K Absence or Antagonist-II (PIVKA-II) for Hepatocellular Carcinoma Screening in Chinese Population

    OpenAIRE

    Yu, Rentao; Ding, Shitao; Tan, Wenting; Tan, Shun; Tan, Zhaoxia; Xiang, Shiqing; Zhou, Yi; Mao, Qing; Deng, Guohong

    2015-01-01

    Background: Alpha-fetoprotein (AFP) has long been used as an effective biomarker for hepatocellular carcinoma (HCC) screening; however, not all HCC patients can be detected with an elevated AFP level, especially in early HCC patients. Protein Induced by vitamin K absence or antagonist-II (PIVKA-II) is another serum biomarker linked to HCC; however, sensitivity and specificity remain controversial and data in Chinese groups is even rarer. Objectives: To evaluate the performance of PIVKA-II alo...

  15. Value of detection of serum AFP, AFU and CEA in diagnosis of primary hepatic cancer%血清GGT、AFP和AFP-L3联合检测对原发性肝癌诊断的临床意义

    Institute of Scientific and Technical Information of China (English)

    李玉柱; 张玉敏; 寇永妹; 陈晖; 韩龙才

    2013-01-01

    目的 探讨r-谷氨酰转移酶(GGT)、血清甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)联合检测对原发性肝癌诊断的诊断价值.方法 正常健康对照组84例,原发性肝癌组92例,良性肝病组45例,均同时检测血清GGT、AFP、AFP-L3.结果 原发性肝癌组血清GGT、AFP、AFP-L3与良性肝病组、正常健康对照组比较,差异有统计学意义(P<0.01).原发性肝癌组血清GGT、AFP、AFP-L3单独检测时的敏感性分别75.5%、71.0%和83.7%,联合检测GGT和AFP或AFP-L3可使检测敏感性提高到79.3%和88.7%,三者联合检测敏感性为93.1%.结论 联合检测GGT、AFP、AFP-L3 3项指标可以大大提高原发性肝癌的诊断率.%Objective To evaluate the value of detection of serum GGT,AFP and AFP-L3 in diagnosis of primary hepatic cancer(PHC).Methods The serum levels of GGT,AFP and AFP-L3 in 92 patients with PHC,84 patients with liver cirrhosis,and 45control subjects were determined and analyzed.Results Serum levels of GGT,AFP and AFP-L3 in patients with PHC were all significantly higher than the control subjects and the patients with liver cirrhosis (P<0.01).The sensitivity of diagnosis of PHC was 75.5%,71.0%,and 83.7%,respectively,when GGT,AFP,and AFP-L3 were individually detected.The accuracy could be increased to 79.3% (combined detection of GGT and AFP) and 88.7% (combined detection of GGT and AFP-L3).The accuracy was further increased to 93.1% when all GGT,AFP and AFP-L3 were detected.Conclusion Combined detection of serum GGT,AFP and AFP-L3 is greatly enhance the sensitivity in diagnosis of PHC,especially in AFP negative patients.

  16. Evaluation of tumor markers for the detection of hepatocellular carcinoma in Yangon General Hospital, Myanmar.

    OpenAIRE

    Taketa K; Okada S.; Win N; Hlaing NK; Wind KM

    2002-01-01

    Levels of alpha-fetoprotein (AFP), its glycoforms AFP-L3 and AFP-P4, and proteins induced by vitamin K absence or antagonist-II (PIVKA-II) were determined in sera obtained from patients in Yangon General Hospital (20 with hepatocellular carcinoma (HCC), 29 with chronic liver diseases, including 3 with chronic hepatitis and 26 with cirrhosis of the liver, and 9 with other hepatobiliary diseases). Forty-five percent of the patients with HCC had serum AFP levels above 10,000 ng/ml, indicating th...

  17. Stem Cells

    Science.gov (United States)

    Stem cells are cells with the potential to develop into many different types of cells in the body. They serve as a repair ... body. There are two main types of stem cells: embryonic stem cells and adult stem cells. Stem ...

  18. Trigger strategies for central exclusive $H \\to b\\overline{b}$ studies with the AFP detector

    CERN Document Server

    Brown, G J A; Kupco, A; Pilkington, A; Tasevsky, M

    2009-01-01

    The ATLAS Forward Proton (AFP) upgrade proposes to install proton detectors at 220 m and 420 m either side of the ATLAS interaction point, turning the LHC into a giant magnetic spectrometer. The physics motivation for this upgrade focuses on final states in which the colliding protons remain intact, allowing a full reconstruction of the event, even in the forward region. One such process is the production of the Higgs boson in the central exclusive channel and tagging the outgoing protons allows the possible extraction of the Higgs quantum numbers, mass and couplings regardless of the decay channel. Studying this exclusive production channel for the presently favoured low Higgs mass depends on the possibility of efficiently triggering, up to the highest luminosities, on a pair of relatively soft jets coming from the decay of b quarks or τ leptons. As jet triggers will inevitably be heavily pre-scaled, even at modest luminosities, it is essential to make a coincidence betweeen information from the tagging d...

  19. Los Determinantes de la Elección de AFP en Chile: Nueva Evidencia a Partir de Datos Individuales

    OpenAIRE

    Berstein Jáuregui, Solange; Cabrita Felix, Carolina

    2006-01-01

    La reforma al sistema chileno de pensiones de 1981, junto con traspasar la administración del nuevo esquema de cuentas individuales a las Administradoras de Fondos de Pensiones (AFP), supone la presencia de compendia entre estas como elemento disciplinador de mercado. Para que este diseño funcione correctamente, es fundamental que los consumidores reaccionen ante cambios a las variables relevantes en este mercado, como precio, rentabilidad y calidad de servicio. Estudios anteriores basados en...

  20. Environmental potential of the use of CO2 from alcoholic fermentation processes. The CO2-AFP strategy.

    Science.gov (United States)

    Alonso-Moreno, Carlos; García-Yuste, Santiago

    2016-10-15

    A novel Carbon Dioxide Utilization (CDU) approach from a relatively minor CO2 emission source, i.e., alcoholic fermentation processes (AFP), is presented. The CO2 produced as a by-product from the AFP is estimated by examining the EtOH consumed per year reported by the World Health Organization in 2014. It is proposed that the extremely pure CO2 from the AFP is captured in NaOH solutions to produce one of the Top 10 commodities in the chemical industry, Na2CO3, as a good example of an atomic economy process. The novel CDU strategy could yield over 30.6Mt of Na2CO3 in oversaturated aqueous solution on using ca. 12.7Mt of captured CO2 and this process would consume less energy than the synthetic methodology (Solvay ammonia soda process) and would not produce low-value by-products. The quantity of Na2CO3 obtained by this strategy could represent ca. 50% of the world Na2CO3 production in one year. In terms of the green economy, the viability of the strategy is discussed according to the recommendations of the CO2Chem network, and an estimation of the CO2negative emission achieved suggests a capture of around 280.0Mt of CO2 from now to 2020 or ca. 1.9Gt from now to 2050. Finally, the results obtained for this new CDU proposal are discussed by considering different scenarios; the CO2 production in a typical winemaking corporation, the CO2 released in the most relevant wine-producing countries, and the use of CO2 from AFP as an alternative for the top Na2CO3-producing countries. PMID:27300565

  1. Environmental potential of the use of CO2 from alcoholic fermentation processes. The CO2-AFP strategy.

    Science.gov (United States)

    Alonso-Moreno, Carlos; García-Yuste, Santiago

    2016-10-15

    A novel Carbon Dioxide Utilization (CDU) approach from a relatively minor CO2 emission source, i.e., alcoholic fermentation processes (AFP), is presented. The CO2 produced as a by-product from the AFP is estimated by examining the EtOH consumed per year reported by the World Health Organization in 2014. It is proposed that the extremely pure CO2 from the AFP is captured in NaOH solutions to produce one of the Top 10 commodities in the chemical industry, Na2CO3, as a good example of an atomic economy process. The novel CDU strategy could yield over 30.6Mt of Na2CO3 in oversaturated aqueous solution on using ca. 12.7Mt of captured CO2 and this process would consume less energy than the synthetic methodology (Solvay ammonia soda process) and would not produce low-value by-products. The quantity of Na2CO3 obtained by this strategy could represent ca. 50% of the world Na2CO3 production in one year. In terms of the green economy, the viability of the strategy is discussed according to the recommendations of the CO2Chem network, and an estimation of the CO2negative emission achieved suggests a capture of around 280.0Mt of CO2 from now to 2020 or ca. 1.9Gt from now to 2050. Finally, the results obtained for this new CDU proposal are discussed by considering different scenarios; the CO2 production in a typical winemaking corporation, the CO2 released in the most relevant wine-producing countries, and the use of CO2 from AFP as an alternative for the top Na2CO3-producing countries.

  2. Reflexiones en Torno a Políticas de Inversión Adecuadas para las Administradoras de Fondos de Pensiones (AFP)

    OpenAIRE

    Eduardo Walker

    1991-01-01

    This paper analyzes the investment strategies that should be followed by the pension fund administrators (AFPs) in Chile in order to maximize objective functions characterized by risk aversion. The investment problem is formally set up and solved for spec

  3. STEM, STEM Education, STEMmania

    OpenAIRE

    Sanders, Mark E.

    2008-01-01

    A series of circumstances has once more created an opportunity for technology educators to develop and implement new integrative approaches to STEM education championed by STEM education reform doctrine over the past two decades.

  4. 亲和层析微柱法测定肝癌特异性AFP及其在肝癌诊断中的临床价值%Quantitative analysis of hepatoma-specific α-fetoprotein (HS-AFP) by a new mini-column affinity chromatography and its clinical value in diagnosis of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Wei Wu; Dengfu Yao; Liwei Qiu; Xiaoxiao Gu; Xinhua Wu

    2008-01-01

    Objective: To establish a convenient and economic method to determine hepatoma-specific α-fetoprotein (HS-AFP) for diagnosis of hepatocellular carcinoma (HCC). Methods: HS-AFP from serum of HCC patients was separated by a mini-column Lens culinaris agglutinin (LCA)-affinity chromatography. The levels of serum total AFP and separated HS-AFP were detected by radioimmunoassay (RIA). Results: Circulating AFP was separated into three peaks (AFP-1, AFP-2, and AFP-3) by LCA-affinity chromatography. During the elution course, the AFP-1 and AFP-2 could be eluted with TE buffer. HS-AFP (AFP-3) from sera of HCC patients was eluted dearly on the LCA-sepharose gel mini-column with a solution containing a-methyl-D-mannoside. It was a part of total AFP and only found in sera of HCC patients. A ratio of more than 15% for HS-AFP to total AFP in serum was considered as a specific marker for HCC diagnosis with higher sensitivity (92.7%) and specificity(88.2%). Conclusion: The new assay for circulating HS-AFP analysis is more sensitive, repeatable, and convenient. Its clinical application would be useful to early diagnosis of HCC.

  5. AFP-L3和AFP联合检测在原发性肝癌诊断价值分析

    Institute of Scientific and Technical Information of China (English)

    胡善雷; 任娜

    2014-01-01

    目的:联合检测不同人群异质体3( AFP- L3)和甲胎蛋白(AFP)的水平,并探讨其对于原发性肝癌的诊断意义。方法采用双抗体夹心酶联免疫法分别检测65例原发性肝癌患者(原发性肝癌组)、65例肝硬化患者(肝硬化组)和65例病毒性肝炎患者(病毒性肝炎组)患者血清中AFP- L3和AFP的表达水平,并选择同期65例健康普查者作为正常对照组。检测各组血清中AFP- L3和AFP的表达水平,并比较AFP- L3、AFP单一检测与AFP- L3和AFP联合检测在原发性肝癌诊断中的阳性率。结果原发性肝癌组患者血清中AFP- L3和AFP水平明显高于肝硬化组和病毒性肝炎组(P<0.05)。原发性肝癌组患者AFP- L3检测阳性率(70.8%)明显高于AFP检测阳性率(64.6%)(P<0.05),AFP- L3和AFP联合检测可提高阳性率(86.2%)(P<0.05)。结论联合检测血清AFP- L3和AFP的水平可提高原发性肝癌诊断的敏感性和特异性,具有重要的临床意义。

  6. Association of liver inflammation with alpha-fetoprotein and treatment response in hepatitis C virus genotype 4 patients

    OpenAIRE

    Samar Samir Youssef; Sameh Mohamed Seif

    2014-01-01

    Objectives: Chronic hepatitis C (CHC) or liver inflammation resulting from infection with hepatitis C virus (HCV) is the cause of chronic liver disease and leads to cirrhosis and hepatocellular carcinoma. The burden of chronic HCV-related liver disease in Egypt continues to rise and the interaction of liver inflammation with biomarkers and response to therapy is scarcely discussed. Moreover, serum alanine transaminase (ALT) is considered as a moderately accurate test for indicating liver infl...

  7. Union examination of AFP,AFU,AFPL3 and γ-GT in early diagnosis of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP),crag algae glycosidase (AFU),armor embryo protein heteroplasmon (AFPL3) and γ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. R...

  8. Clonogenic colony-forming ability of hepatic stem cells in the spleens of mice

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    To confirm the existence of hepatic stem cells (HSCs), fetal liver cells isolated from mice on embryonic day 13 (ED13) were long-term cultured in vitro. Growth of the cells was observed intensively and characteristics were identified by immunocytochemistry. The results showed that some of the cells grew as colonies, in which some cells expressed AFP, CD34 and Albumin. Then the cells were transplanted intravenously into irradiated syngeneic mice. At day 12 a number of small hyperplasia nodules were seen in the apparently enlarged spleens of recipient mice. Moreover, some nodules were positive for AFP and CD34 and consisted of various types of cells, suggesting the very existence of hepatic stem cells in the mouse fetal liver.

  9. AFP-L3和AFP联合检测在原发性肝癌诊断价值分析

    Institute of Scientific and Technical Information of China (English)

    胡善雷; 任娜

    2014-01-01

    目的:联合检测不同人群异质体3( AFP- L3)和甲胎蛋白(AFP)的水平,并探讨其对于原发性肝癌的诊断意义。方法采用双抗体夹心酶联免疫法分别检测65例原发性肝癌患者(原发性肝癌组)、65例肝硬化患者(肝硬化组)和65例病毒性肝炎患者(病毒性肝炎组)患者血清中AFP- L3和AFP的表达水平,并选择同期65例健康普查者作为正常对照组。检测各组血清中AFP- L3和AFP的表达水平,并比较AFP- L3、AFP单一检测与AFP- L3和AFP联合检测在原发性肝癌诊断中的阳性率。结果原发性肝癌组患者血清中AFP- L3和AFP水平明显高于肝硬化组和病毒性肝炎组(P<0.05)。原发性肝癌组患者AFP- L3检测阳性率(70.8%)明显高于AFP检测阳性率(64.6%)(P<0.05),AFP- L3和AFP联合检测可提高阳性率(86.2%)(P<0.05)。结论联合检测血清AFP- L3和AFP的水平可提高原发性肝癌诊断的敏感性和特异性,具有重要的临床意义。

  10. 肝病患者AFP、GP73、AFP-L3和AFU的表达及其相关性

    Institute of Scientific and Technical Information of China (English)

    臧桂珍; 李丹; 丁艳

    2015-01-01

    目的 通过测定血清中甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)、高尔基体蛋白73(GP73)和α-L-岩藻糖苷酶(AFU)的水平,比较它们在不同肝脏疾病中的意义,原发性肝癌(PHC)患者中不同水平AFP值与AFP-L3、GP73和AFU三者异常率的相关性.方法 将734例肝病患者分为PHC组(A组)271例;慢性肝炎组(B组)254例;肝硬化组(C组)209例,另选208例健康体检者(D组),测定血清中的GP73、AFP-L3、AFP和AFU水平.结果 三组患者的GP73、AFP-L3和AFP水平及异常率较D组增高(P<0.05);A组和B组的GP73水平及异常率较C组低(P<0.05);B组和C组AFP-L3和AFP水平及异常率低于A组(P<0.05);在A组中,AFP-L3的异常率与AFP呈正相关(r=0.81),AFU的异常率与AFP呈正相关(r=0.83),但GP73的异常率与AFP无明显相关性.结论 GP73更适用于肝硬化鉴别诊断;AFP-L3在诊断PHC中有较高的价值;当AFP值>100 ng/ml时,AFP-L3异常率维持在较高水平.

  11. Diagnostic value of serum Fer, AFP and AFP-L3 combined detection in primary hepatic cancer%血清铁蛋白、甲胎蛋白和甲胎蛋白异质体联合检测对原发性肝癌诊断的临床意义

    Institute of Scientific and Technical Information of China (English)

    李玉柱; 张玉敏

    2014-01-01

    目的 探讨血清铁蛋白(Fer)、甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)联合检测对原发性肝癌的诊断价值.方法 健康对照组84名、原发性肝癌组92例、良性肝病组45例均同时检测血清Fer、AFP、AFP-L3.结果 原发性肝癌组血清Fer检测结果为(819.4±616.8)μg/L,AFP为(556.9±451.5)μg/L,AFP-L3为(109.0±62.5) μg/L,良性肝病组Fer检测结果为(229.3±155.5) μg/L,AFP为(6.0±4.2) μg/L,AFP-L3为(26.0±49.4) μg/L,健康对照组Fer检测结果为(125.6±110.5)μg/L,AFP为(2.8±0.8) μg/L,AFP-L3为(7.0± 1.8)μg/L,三者差异有统计学意义(均P<0.01).原发性肝癌组单独检测血清Fer的敏感度为85.9%,AFP的敏感度为71.0%,AFP-L3的敏感度为83.7%,联合检测Fer和AFP或AFP-L3可使检测敏感度提高到90.2%和95.7%,三者联合检测敏感度为98.9%.结论 联合检测血清Fer、AFP、AFP-L3三项指标可以提高原发性肝癌的诊断准确率.%Objective To evaluate the diagnostic value of the combined detection of serum Fer,AFP and AFP-L3 in patients with primary hepatic cancer (PHC).Methods The serum levels of Fer,AFP and AFP-L3 were determined in 92 patients with PHC,84 patients with benign liver disease,and 45 control subjects.Results The PHC group serum Fer was (819.4±616.8) μg/L,AFP was (556.9±451.5) μg/L,AFP-L3 was (109.0±62.5) μg/L.The benign liver disease group Fer was (229.3±155.5) μg/L,AFP was (6.0±4.2) μg/L,AFP-L3 was (26.0±49.4) μg/L.The healthy control group Fer was (125.6±110.5) μg/L,AFP was (2.8±0.8) μg/L,AFP-L was (7.0±1.8) μg/L,there was significant difference between the three groups (all P < 0.01).The sensitivity of PHC group alone in detection of serum Fer was 85.9 %,the sensitivity of AFP was 71.0 %,the sensitivity of AFP-L3 was 83.7 %,the combined detection of Fer and AFP or AFP-L3 improved the sensitivity of the test to 90.2 % and 95.7 %,the three combined detection sensitivity was 98.9 %.Conclusion Combined

  12. The mesenchymal transcription factor SNAI-1 instructs human liver specification.

    Science.gov (United States)

    Goldman, Orit; Valdes, Victor Julian; Ezhkova, Elena; Gouon-Evans, Valerie

    2016-07-01

    Epithelial-mesenchymal transition (EMT) and the mesenchymal-epithelial transition (MET) are processes required for embryo organogenesis. Liver develops from the epithelial foregut endoderm from which the liver progenitors, hepatoblasts, are specified. The migrating hepatoblasts acquire a mesenchymal phenotype to form the liver bud. In mid-gestation, hepatoblasts mature into epithelial structures: the hepatocyte cords and biliary ducts. While EMT has been associated with liver bud formation, nothing is known about its contribution to hepatic specification. We previously established an efficient protocol from human embryonic stem cells (hESC) to generate hepatic cells (Hep cells) resembling the hepatoblasts expressing alpha-fetoprotein (AFP) and albumin (ALB). Here we show that Hep cells express both epithelial (EpCAM and E-cadherin) and mesenchymal (vimentin and SNAI-1) markers. Similar epithelial and mesenchymal hepatoblasts were identified in human and mouse fetal livers, suggesting a conserved interspecies phenotype. Knock-down experiments demonstrated the importance of SNAI-1 in Hep cell hepatic specification. Moreover, ChIP assays revealed direct binding of SNAI-1 in the promoters of AFP and ALB genes consistent with its transcriptional activator function in hepatic specification. Altogether, our hESC-derived Hep cell cultures reveal the dual mesenchymal and epithelial phenotype of hepatoblast-like cells and support the unexpected transcriptional activator role of SNAI-1 in hepatic specification. PMID:27240252

  13. Clinical Significance of AFP and PIVKA-II Responses for Monitoring Treatment Outcomes and Predicting Prognosis in Patients with Hepatocellular Carcinoma

    Directory of Open Access Journals (Sweden)

    Hana Park

    2013-01-01

    Full Text Available Aim. Recently, the utility of tumor markers in the hepatocellular carcinoma (HCC field has received a good deal of attention. Here, we review and summarize the results of studies on the roles played by the α-fetoprotein (AFP and prothrombin induced by the absence of vitamin K or antagonist-II (PIVKA-II responses in terms of the monitoring of outcomes and prediction of prognosis after various HCC treatments. Methods. Studies lodged in PUBMED and that satisfied our inclusion criteria were reviewed. Results. We reviewed 12 studies measuring both AFP and PIVKA-II responses in HCC patients treated in various ways. The results are presented by treatment modality. Conclusion. Measurement of AFP and PIVKA II marker levels before and after HCC treatment is clinically useful in monitoring of treatment outcomes and prognosis and in predicting recurrence and survival.

  14. 3) 肝細胞癌の血清学的診断(シンポジウム 腫瘍抗原の臨床診断への応用, 第452回新潟医学会)

    OpenAIRE

    鈴木, 康史; 板谷, 啓司; 青柳, 豊; 小黒, 仁; 横田, 剛; 斉藤, 敦; 五十嵐, 健太郎; 上村, 朝輝; 朝倉, 均; Suzuki, Yasufumi; Itaya, Keiji; Aoyagi, Yutaka; Oguro, Makoto; Igarashi, Kentarou; Saitou, Atsushi

    1990-01-01

    The serum concentration and degree of fucosylation (Fucosylation index) of alpha-fetoprotein (AFP) were determined in serum samples from 322 patients with hepatocellular carcinoma (HCC) and 155 with benign liver diseases (BLD). The serum concentration of PIVKA-II, sialyl SSEA (SLX) and CA-50 were also determined 60 with HCC and 60 with BLD. When the serum AFP concentration was below 1000ng/ml, there was no demarcation line between HCC and BLD. However, the fucosylation index of AFP proved use...

  15. 肝细胞癌合并门静脉癌栓与 AFP-L3% 相关性研究%Correlation between Lens Culinaris Agglutlnin -reactive A -Fetoprotein( AFP -L3 % ) with Portal Vein Tumor Thrombosis in Hepatocellnlar Carcinoma

    Institute of Scientific and Technical Information of China (English)

    陈祖华; 陈瑶; 高智峰; 徐爱芳; 李运江

    2012-01-01

    目的 探讨肝细胞肝癌(hepatocellular carcinoma,HCC)伴门静脉癌栓(portal vein tumor thrombosis,PVTT)与AFP-L3%相关性.方法 将治疗前15天内完成多排螺旋CT(MSCT)检查和AFP-L3%检测的HCC患者100例作为研究对象.根据AFP - L3%检测结果,分为阳性组(≥10%)和阴性组(<10%).PVTT诊断和累及范围通过多排螺旋CT增强3期检查.根据PVTT累及范围分为Ⅳ级:Ⅰ级为门静脉一级分支,即门静脉左或右干(LPV、RPV)有瘤栓;Ⅱ级LPV或RPV加门静脉主干(TPV)被侵犯;Ⅲ级LPV、RPV和TPV均受累;Ⅳ级PVTT加脾静脉或肠系膜上静脉被累及,合并肝外转移;并计算各级癌栓AFP-L3%均值.AFP - L3采用装有偶联了小扁豆凝集素(LCA)的微量离心柱分离甲胎蛋白,利用化学发光法检测AFP和AFP -L3,并计算AFP -L3占AFP含量的百分比.采用SPSS17.0软件进行统计学分析.结果 在100例HCC患者中,阳性组71例(71%),其中合并PVTT 41例(57.7%);阴性组29例(29%),合并PVTT8例(27.6%).阳性组较阴性组易侵犯门静脉形成PVTT(P <0.01).根据PVTT累及范围分级,Ⅰ级16例、Ⅱ级13例、Ⅲ级11例及Ⅳ级9例.Ⅰ~Ⅳ级AFP - L3%均值分别为14.5、18.3、20.1、26.3.PVTT各级间的AFP - L3%均值有统计学意义(P<0.05).结论 AFP - L3%阳性HCC患者易侵犯门静脉形成PVTT,并且AFP - L3%越高PVTT累及范围越广.%To investigate correlation between Lens culinaris agglutinin - reactive a - Fetoprotein( AFP - 13% ) and portal vein tumor thrombosis ( PVTT) in hepatocellular carcinoma(HCC) . Methods A total of 100 patients with HCC who were examinated AFP - L3% and multidetector CT( MDCT) in 15 days before treatment were regarded as research object. AFP - L3 of 10% or higher was judged to the positive group,otherwise, to the negative group. AFP -L3% were acquired by lectin - affinity electrophoresis coupled with antibody - affinity blotting, and expressed as AFP - 1.3 percentage of total

  16. UTJECAJ SPOLA FETUSA I PUŠENJA TE RODNOSTI MAJKE NA AFP I SLOBODNI beta-HCG U PLODOVOJ VODI UREDNIH TRUDNOĆA DRUGOG TROMJESEČJA

    OpenAIRE

    Brajenović-Milić, Bojana; Tišljarić, Dubravka; Petrović, Oleg; Prodan, Mirko; Ruistić, Smiljana; Marout, Jasminka; Herman, Radoslav; Kapović, Miljenko

    2007-01-01

    Cilj istraživanja. (1) Ispitati utjecaj pušenja i rodnosti (pariteta) majke i spola fetusa na razinu alfa-fetoproteina (AFP) i slobodne beta-podjedinice humanog korionskog gonadotropina (slobodni beta-hCG) u plodovoj vodi urednih trudnoća. (2) Ispitati korelacije između serumskih vrijednosti AFP i slobodnog beta-hCG i vrijednosti spomenutih biljega u plodovoj vodi. Ispitanice i metode. Ispitivanje je provedeno na 233 uzorka plodove vode koji su dobiveni amniocentezom u drugom tromjesečju trud...

  17. 微量离心柱法分离检测甲胎蛋白异质体(AFP-L3)在肝癌诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    刘小壮; 刘庆丰

    2012-01-01

      目的:应用微量离心柱法检测甲胎蛋白异质体(AFP-L3),探讨AFP-L3在肝癌鉴别诊断中的意义.方法:应用预装有偶联小扁豆凝集素(LCA)的琼脂糖微量离心柱分离AFP-L3,采用时间分辨免疫荧光法检测AFP和AFP-L3,并计算AFP-L3值.以AFP〉20ng/L,AFP-L3〉10%作为阳性判断标准.结果:肝细胞癌患者血清中AFP、AFP-L3水平及阳性率明显高于其他肝病患者(P〈0.01).结论:微量离心柱法测定AFP-L3(%)在AFP阳性时的良、恶性肝病的鉴别及肝癌的早期诊断具有重要意义.

  18. PENGKAJIAN DATA RUMAH SAKIT (HOSPITAL RECORD REVIEW KASUS ACUTE FLACCID PARALYSIS (AFP TAHUN 1999-2000 DI JAWA TIMUR

    Directory of Open Access Journals (Sweden)

    Cholis Bachroen

    2012-11-01

    Full Text Available This survey was the evaluation of the program on Polio Eradication through Acute Flaccid Paralysis (AFP Surveillance especially Hospital Based Surveillance. The evaluation was done by reviewing the Hospitals' Record (Hospital Based Survey. The objective of the survey was to estimate the under reported of routine reporting system, which the data of the survey used as a gold standard. The results showed that due to incomplete of the records in several hospitals, some of AFP cases might be could not be covered. However the under reported of the routine surveillance system was more than 50%. It seems that the strengthening of supervision was still needed to increase coverage of the routine surveillance system.   Keywords: hospitals; medical record; acute flocid paralysis

  19. Immature teratoma with microscopic yolk sac tumor: A case of diagnostic challenge on histology

    OpenAIRE

    Sonali R Saraf; Madhu Chaturvedi; Manisha Khare; Alka Kalgutkar

    2016-01-01

    An adolescent girl with raised alpha-fetoprotein (AFP) levels presented with a left-sided large solid ovarian mass. Microscopically, the tumor was composed of a variety of immature and mature tissues derived from the three germ layers having very sparse (single focus of) immature neuroepithelium. Few areas also showed the presence of a reticular pattern of yolk sac tumor (YST). Hence, the tumor was diagnosed as immature teratoma with microscopic foci of yolk sac elements. After 6 months of ci...

  20. Identification of Serum Monocyte Chemoattractant Protein-1 and Prolactin as Potential Tumor Markers in Hepatocellular Carcinoma

    OpenAIRE

    Who-Whong Wang; Soo Fan Ang; Rajneesh Kumar; Charmain Heah; Andi Utama; Navessa Padma Tania; Huihua Li; Sze Huey Tan; Desmond Poo; Su Pin Choo; Wan Cheng Chow; Chee Kiat Tan; Han Chong Toh

    2013-01-01

    Early diagnosis of hepatocellullar carcinoma (HCC) remains a challenge. The current practice of serum alpha-fetoprotein (AFP) measurement is inadequate. Here we utilized a proteomic approach to identify novel serum biomarkers for distinguishing HCC patients from non-cancer controls. We profiled the serum proteins in a group of 58 resectable HCC patients and 11 non-HCC chronic hepatitis B (HBV) carrier samples from the Singapore General Hospital (SGH) using the RayBio® L-Series 507 Antibody Ar...

  1. AFP、AFP-L3、GP73联合检测在肝癌诊断中的应用%The application of combine detection of AFP、AFP-L3 and GP73 in the diagnosis of hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    王向臣; 曲建香; 鹿理友; 于传亭; 林青

    2011-01-01

    目的 检测不同患者样本中甲胎蛋白(AFP)、甲胎蛋白异质体(AFP-L3)以及高尔基体蛋白73(GP73)的含量,研究AFP、AFP-L3、GP73在肝癌诊断中的应用.方法 收集肝癌患者样本、肝炎肝硬化样本以及正常健康人员样本,用电化学发光法检测AFP、AFP-L3及用ELISA法检测GP73含量.结果 1.本研究显示:AFP-L3%>10%对肝癌诊断敏感度为89.1%;诊断特异度为94.4%[1];2.在101例肝癌标本中,GP73的阳性率为72.28%,AFP的阳性率37.62%,经过卡方检验,两者具有显著性差异(χ2=18.86,p<0.01);3.在所有非肝癌患者中,GP73的诊断特异性为93.44%;AFP的诊断特异性为86.48%.结论 AFP-L3、GP73 用于诊断肝癌效果明显优于AFP,可以显著提高对肝癌诊断的灵敏度和特异性,而且AFP-L3、GP73 相比AFP更不容易受到肝部炎症和其他部位肿瘤的影响.结合本人早期研究结果,将GP73、AFP-L3、AFP联合起来检测,能更好更快的对肝癌做出早期诊断.

  2. Stem Cells

    OpenAIRE

    Madhukar Thakur

    2009-01-01

    Objective: The objective of this presentation is to create awareness of stem cell applications in the ISORBE community and to foster a strategy of how the ISORBE community can disseminate information and promote the use of radiolabeled stem cells in biomedical applications. Methods: The continued excitement in Stem Cells, in many branches of basic and applied biomedical science, stems from the remarkable ability of stem cells to divide and develop into different types of cells in ...

  3. Types of Stem Cells

    Science.gov (United States)

    ... PDF) Download an introduction to stem cells and stem cell research. Stem Cell Glossary Stem cell terms to know. ... stem cells blog from the International Society for Stem Cell Research. Learn About Stem Cells From Lab to You ...

  4. Relations between ZHX2, NF-YA and AFP expression in HCC tissues%肝细胞癌中ZHX2、NF-YA及AFP表达的相关关系

    Institute of Scientific and Technical Information of China (English)

    胡少为; 杨海峰; 王智园; 袁丽娜; 文剑明

    2011-01-01

    目的 检测肝细胞癌(hepatocellular carcinoma,HCC)组织中ZHX2、NF-YA及AFP mRNA和蛋白表达的相关关系.方法 采用RT-PCR及免疫组织化学方法检测HCC组织中ZHX2、NF-YA及AFP mRNA和蛋白的表达情况.结果 ZHX2 mRNA在AFP mRNA阴性的25例HCC组织中的表达率为52.0%,明显高于AFP mRNA阳性组织中的表达率(26.3%,P=0.038),ZHX2 mRNA与AFP mRNA表达呈负相关(OR=0.33);NF-YA mRNA与ZHX2 mRNA表达呈正相关(OR=31.429),而与AFP mRNA表达呈负相关关系(OR=0.308).AFP蛋白阴性的142例HCC组织中,ZHX2蛋白的表达率为23.9%,明显高于在AFP蛋白阳性组织中的表达率(12.8%,P=0.034);HCC组织中ZHX2蛋白与AFP蛋白表达呈负相关(P=0.018,r=-0.153).NF-YA蛋白在HCC组织中与ZHX2蛋白表达呈正相关(P=0.000,r=0.371),与AFP蛋白表达呈负相关(P=0.000,r=-0.497).结论 联合检测AFP和ZHX2蛋白将提高HCC的诊断率;ZHX2可能是通过NF-YA来调控AFP的表达.%Purpose To study the relations between ZHX2 , NF-YA and AFP expression in HCC tissues.Methods We detected mRNA and protein expression of ZHX2.NF-YA and AFP in HCC tissues by RT-PCR and immunohistochemistry.Results ZHX2 mRNA expression rate ( 52.0% ) in HCC tissues without AFP mRNA expression was significantly higher than that ( 26.3% ) with AFP mRNA expression ( P = 0.038 ).There was a negative relationship between ZHX2 and AFP mRNA expression ( OR = 0.33 ).In addition.NFYA mRNA expression was positively correlated with ZHX2 mRNA expression ( OR = 31.429 ) , and negatively correlated with AFP mRNA expression ( OR = 0.308 ).The expression rate of ZHX2 protein in HCC tissues without AFP protein expression was 23.9%.which was significantly higher than that with AFP protein expression ( 12.8% , P = 0.034 ).ZHX2 protein expression was negatively correlated with AFP protein expression ( P = 0.018.r = - 0.153 ).In addition.NF-YA protein expression was positively correlated with ZHX2 protein expression ( P = 0.000.r = 0

  5. Construction of plasmid vector pAFP-HSVtk-IRES2-EGFP and its effect on the cytotoxicity of ganciclovir to hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    Lai Zhiyong; Qin Qin; Yu Baofeng; Xie Jun; Gao Ranpeng; Zhang Tiantian; Li Chunfeng

    2014-01-01

    Background Herpes simplex virus thymidine kinase phosphorylates ganciclovir to ganciclovir monophosphate,which is then converted to ganciclovir triphosphate by endogenous cellular nucleoside kinases.The ganciclovir triphosphate acts as a DNA chain terminator due to the lack of a functional 3'-OH group and terminates the process of DNA replication,hence leading to cell apoptosis.At present,HSVtk gene usually acts as suicide gene to kill tumor cells.The aim of this study was to investigate the selective cytotoxicity of the herpes simplex virus thymidine kinase/ganciclovir (HSVtK/GCV) suicide gene system controlled by the α-fetoprotein (AFP) promoter on hepatocellular carcinoma (HCC) cells in vitro.Methods pAFP-HSVtk-IRES2-EGFP recombinant plasmid vectors driven by the AFP promoter were constructed.HL-7702 liver cells,HUH-7 HCC,and HepG2 HCC were transfected with the recombinant plasmids.HSVtK gene expression was detected using Western blotting analysis.HepG2 cells line stably expressing HSVtk gene was selected by G418 reagent.The cytotoxicity of HSVtK/GCV suicide gene system on hepatoma cells was measured by CCK-8 reagents when different doses of ganciclovir were added.Results Plasmid pAFP-TK-IRES2-EGFP-expressed HSVtk gene was constructed successfully.HSVtk gene expression level was significantly higher in AFP-positive hepatoma cells than in AFP-negative liver cells.After G418 selection,a HepG2 cells line stably expressing HSVtk gene was acquired.With the increase of the dose of ganciclovir the optical density at 450 nm of HepG2 cells stably expressing HSVtk gene gradually decreased (P <0.05).Conclusion The HSVtK gene-specific expression in hepatoma cells as well as the cytotoxicity of the suicide gene system in HepG2 cells provided the basis for the targeted gene therapy of HCC.

  6. Stem system

    Directory of Open Access Journals (Sweden)

    Shajmardanova L.R.

    2008-01-01

    Full Text Available The article shows the stem cells from the point of view of systemic anatomy, where they are united into an entire stem system. This integration shows various advantages for better understanding the stem cells role in the body. Authors, considering functions and action mechanism of stem cells, phylogeny, ontogeny, regulation of functions, explain the base for stem cells system existance. The definition and terminology, age peculiarities, relationship with other systems of organs and differences are offered. The theoretical proof of primary-integrative role of stem system is provided for all other systems in body. Of particular importance is the role of stem system as a link between neural and endocrine systems. The work helps to sistematize data on investigations of stem cells, their entire-system perception, that will ease the goals determining for further research and their clinical use.

  7. Clinical Significance of AFP and PIVKA-II Responses for Monitoring Treatment Outcomes and Predicting Prognosis in Patients with Hepatocellular Carcinoma

    OpenAIRE

    Hana Park; Jun Yong Park

    2013-01-01

    Aim. Recently, the utility of tumor markers in the hepatocellular carcinoma (HCC) field has received a good deal of attention. Here, we review and summarize the results of studies on the roles played by the α -fetoprotein (AFP) and prothrombin induced by the absence of vitamin K or antagonist-II (PIVKA-II) responses in terms of the monitoring of outcomes and prediction of prognosis after various HCC treatments. Methods. Studies lodged in PUBMED and that satisfied our inclusion criteria were r...

  8. Time courses of PIVKA-II and AFP levels after hepatic artery embolization and hepatic artery infusion against hepatocellular carcinoma: relation between the time course and tumor necrosis.

    Science.gov (United States)

    Kishi, K; Sonomura, T; Mitsuzane, K; Nishida, N; Kimura, M; Satoh, M; Yamada, R; Kodama, N; Kinoshita, M; Tanaka, H

    1992-01-01

    We examined 35 untreated patients with unresectable hepatocellular carcinoma who exhibited positivity for both plasma PIVKA-II and serum AFP, and studied the weekly course of these markers from the pre-TAE or -HAI period to the third week of treatment. We correlated changes in these markers with the tumor necrosis rate and the time course on X-ray CT images. One week after TAE, the tumor necrosis rate and the time course of PIVKA-II showed a significant correlation (r = 0.7), while the correlation was between the time course of AFP and the tumor necrosis rate was insignificant (r = 0.2). At two and three weeks after TAE, both the time course of AFP and PIVKA-II showed significant correlations with the tumor necrosis rate. In 16 patients with tumor necrosis rates of not less than 90%, the mean of the actual half-life (AHL) of PIVKA-II was 3.2 days, the shortest was 1.83 days, and 75% of all AHLs clustered from two days to four days, while the mean and shortest AHLs of AFP were six days and 2.98 days, respectively, exhibiting a broader distribution. On the other hand, in three out of the nine cases of hepatocellular carcinoma complicated with portal tumor thrombi, PIVKA-II increased after HAI in spite of a reduction in tumor size. It was suggested that the PIVKA-II level requires careful interpretation in cases of portal vein obstruction after intensive hepatic arterial infusion of anticancer agents.

  9. 肿瘤异常蛋白与AFP mRNA检测在肝癌诊断中的价值

    Institute of Scientific and Technical Information of China (English)

    张锐

    2010-01-01

    目的:探讨肿瘤异常蛋白(Tumour abnormal protein,TAP)、 AFP mRNA对肝癌的诊断价值.方法: TAP测定利用末梢血,借助特殊凝聚助剂,用显微镜观察特异形态凝聚物,按形态结果分为阳性、阴性及临界.AFP mRNA荧光定量PCR法检测.结果:原发性肝癌TAP阳性率为94%(47/50),明显高于肝硬化20%(10/50)及慢性肝炎10%(5/50)阳性率.16例AFP阴性TAP阳性率为81%(13/16).6例肝癌手术切除2周后TAP逐渐转阴,而用肝动脉栓塞化疗术则TAP不转阴,AFP mRNA早期肝癌Ⅰ期6例、Ⅱ期11例阳性,50例肝癌患者中72%(36/50)阳性,其中肝外转移阳性率100%(30/30),无肝外转移阳性率45%(9/20),慢性肝炎10%(5/50),肝硬化22%(11/50).结论:TAP、 AFP mRNA在慢性活动性肝炎和肝硬化的恶变和肝癌的早期诊断中有一定的意义

  10. 肝細胞癌診断におけるPIVKA-IIならびに各種腫瘍マーカー測定の臨床的意義とその糖鎖変異に関する研究

    OpenAIRE

    小黒, 仁; Oguro, Makoto

    1992-01-01

    The plasma concentrations of PIVKA-II in samples from 155 patients with HCC and 298 with other liver diseases were determined. The serum concentrations and fucosylation index of alpha-fetoprotein (AFP), sialyl stage-specific embryonic antigen-1 (SLX), CA-50, and Dupan-2 were also determined in 60 patients with HCC, 70 with nonneoplastic liver diseases and 25 with carcinoma metastatic to the liver. In 16 HCC patients with positive reactions of both PIVKA-II and AFP longitudinal series of their...

  11. Spontaneous Regression of Hepatocellular Carcinoma due to Disruption of the Feeding Artery

    OpenAIRE

    Okano, Akihiro; Ohana, Masaya; Kusumi, Fusako; Nabeshima, Motoshige

    2013-01-01

    We present an unusual case of spontaneous regression of hepatocellular carcinoma (HCC). A 77-year-old man with alcoholic liver cirrhosis presented with a 50-mm tumor in the Couinaud's segment 8 (S8) of the liver, a 15-mm tumor in the S8-7 and 10-mm tumors in the other segments (S4, S6). The tumors were diagnosed as HCC by typical imaging findings and elevated serum alpha-fetoprotein (AFP, 1,825.0 ng/ml) and protein induced by vitamin K absence II (PIVKA II, 3,043 mAU/ml). One month later, AFP...

  12. Alfa-fetoprotein secreting ovarian sex cord-stromal tumor

    Directory of Open Access Journals (Sweden)

    Kusum D Jashnani

    2013-01-01

    Full Text Available Ovarian sex cord-stromal tumors are relatively infrequent neoplasms that account for approximately 8% of all primary ovarian tumors. They are a heterogeneous group of neoplasms composed of cells derived from gonadal sex cords (granulosa and Sertoli cells, specialized gonadal stroma (theca and Leydig cells, and fibroblasts. They may show androgenic or estrogenic manifestations. We report such a tumor associated with markedly raised serum alpha-fetoprotein (AFP levels in a young female presenting with a mass and defeminising symptoms. Serum AFP levels returned to normal on removal of tumor.

  13. Stem Cells

    Directory of Open Access Journals (Sweden)

    Madhukar Thakur

    2015-02-01

    Full Text Available Objective: The objective of this presentation is to create awareness of stem cell applications in the ISORBE community and to foster a strategy of how the ISORBE community can disseminate information and promote the use of radiolabeled stem cells in biomedical applications. Methods: The continued excitement in Stem Cells, in many branches of basic and applied biomedical science, stems from the remarkable ability of stem cells to divide and develop into different types of cells in the body. Often called as Magic Seeds, stem cells are produced in bone marrow and circulate in blood, albeit at a relatively low concentration. These virtues together with the ability of stem cells to grow in tissue culture have paved the way for their applications to generate new and healthy tissues and to replace diseased or injured human organs. Although possibilities of stem cell applications are many, much remains yet to be understood of these remarkable magic seeds. Conclusion: This presentation shall briefly cover the origin of stem cells, the pros and cons of their growth and division, their potential application, and shall outline some examples of the contributions of radiolabeled stem cells, in this rapidly growing branch of biomedical science

  14. AFP和DCP在肝癌患者预后评价中的作用%Function of AFP and DCP in evaluating the prognosis of hepatocarcinoma

    Institute of Scientific and Technical Information of China (English)

    陈继军; 杨宏勋; 李超; 赵兵刚; 韩强; 赵鹏; 尹文

    2013-01-01

    Objective To evaluate the prognosis in patients with hepatocarcinoma by examining the expression of α-fetoprotein (AFP) and des γ prothrombin (DCP).Methods Retrospectively analyzed the expression of AFP and DCP in 105 patients received curative hepatectomy.Divided the patients into three groups as follow:the tumor markers were both negative pre-and post-operation (Group N) ; the tumor markers decreased to normal after operation (Group D) ; the tumor markers kept positive or decreased but still higher positive after operation (Group P).Then combined the two markers and divided the patients into 4 groups:two markers both negative(AFP + DCP +)(Group A) ; AFP + DCP-(Group B) ; AFP-DCP + (Group C) ; AFP-DCP-(Group D).Results High AFP and DCP levels were significantly associated with poor tumor-free and overall survival.The presence of large size and advanced stage were significantly associated with Group P.Overall survival in the AFP-N group was significantly better than that of other groups and overall survival in DCP-N group were significantly better than that of the P group.After the combination,Group A had the best overall and tumor free survival rate while the D group had the worst.The differences between B and C group were not significant.Conclusions AFP and DCP can be both used solely as tumor markers and the expressions of them are associated with the prognosis.The combination of two markers can be used for better prediction of hepatocarcinoma.%目的 通过检测肝癌患者术后甲胎蛋白(AFP)和γ-羧基凝血酶原(DCP)水平的变化情况,评估AFP和DCP在肝癌患者预后评价中的作用.方法 回顾性分析105例接受根治性切除的肝癌患者术前及术后AFP和DCP水平,并根据两指标变化情况将患者分为3组:治疗前后在正常参考值范围内(N组);治疗前水平高于正常参考值上限,但治疗后回降至正常范围内(D组);治疗前水平正常,但治疗后高于正常参考值上限及治疗前水平高

  15. 肝癌和肝病患者血清甲胎蛋白异质体的检测及临床意义%The Significance of Measurement of AFP-L3 Variant in Patients with hepatocellular carcinoma and liver diseas

    Institute of Scientific and Technical Information of China (English)

    戴亚新; 张时良; 裴豪

    2013-01-01

    Objective To evaluate the clinical significance of scrum AFP hctcrogcncity(AFP-L3) assay in patients with hcpatoccllular carcinoma. Methods Lentil lcctin-binding scrum AFP-L3 variants were separated by microccntrifu-gal column,and the content percentage of AFP-L3(AFP-L3%) variants and total scrum AFP was detected by chcmilu-mincsccncc analysi. Results When AFP-L3%>10% was used as a positive criterion,there was a positive rate of 70% in 27 cases of hcpatoccllular carcinoma(HCC) , which in cirrhosis and chronic hepatitis were 10% and 9. 6%rcspcctivc-ly. AFP-L3% levels were the highest in hcpatoccllular carcinoma than those in cirrhosis and chronic hepatitis (P25 μg/L was used as a positive criterion, the AFP positive rates were 78%, 60% and 55%, in HCC,cirrhosis and chronic hepatitis respectively. The sensitivity of AFP and AFP-L3 was 78% ,70% ,thc speciality of AFP ang AFP-L3 was 43% ,90% ,in HCC respectively. Conclusion AFP-L3 is very useful in the differential diagnosis of hcpatoccllular carcinoma.%目的 探讨甲胎蛋白异质体(AFP-L3)检测在肝癌鉴别诊断中的意义.方法 应用微量离心柱法分离检测小扁豆凝集素结合型AFP-L3的含量,同时检测血清中AFP总含量,由此得出AFP-L3的百分含量(AFP-L3%).结果 以AFP-L3%>10%作为阳性判断标准,27例肝癌患者血清AFP-L3阳性率为70%,肝硬化和慢性肝炎AFP-L3%分别为10%和9.6%,肝癌AFP-L3%水平明显高于肝硬化和慢性肝炎(P<0.01);以AFP>25 ng/ml作为阳性判断标准,肝癌、肝硬化和慢性肝炎患者血清中AFP阳性率分别为78%、60%和55%;以AFP、AFP-L3%作为诊断肝癌指标的灵敏度分别是78%、70%,特异度分别是43%、90%.结论 AFP-L3%能有效区分肝癌和慢性肝病,弥补肝癌诊断的不足,具有一定的临床实用价值.

  16. 甲胎蛋白异质体L3预警原发性肝癌的研究%The studies on early warning of Lens culinaris-reactive α-Fetoprotein (AFP-L3) detecting in primary hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    李永利; 马洪滨; 郭静霞; 史佳彬; 徐军; 刘爱霞; 杨丽华; 李伯安; 毛远丽

    2008-01-01

    Objective To explore the significance of Lens culinaris-reactive α-Fetoprotein (AFP-L3) detection in primary hepatocellular carcinoma. Methods AFP-L3 was isolated by using microspincolumn coupled with lens culinaris agglutinin (LCA), AFP and AFP-L3 were detected with chemiluminescent immunoassay, the propertion of AFP L3 levels were calculated, and the relationship between the elevated AFP-L3 (%) levels and benign and malignant liver disease was analyzed. Results There were significant differences in positive rate between the patients of HCC, suspected HCC and other liver disease (81.80%, 73.68%, 11.80%, respectively, P < 0.05). Among the undetermined HCC (suspected HCC, liver disease) patients, 12 out of 21 cases of AFP-13 positive were diagnosed to be HCC within 6 months, and 6 of them were diagnosed to be the single small HCC at the early stage through B-Ultrasonic Diagnosis or CT. Among 62 cases of AFP-L3 negative, 3 cases were diagnosed to be HCC within 6 months and the risk of occurrence of HCC for AFP-L3 positive increased 11.9 times. Conclusion AFP-L3 has no correlation with AFP value, and it can be used as an independent HCC diagnosis factor. The detection of AFP-L3 has a significant implication for the identification of benign or malignant liver disease and the early stage predictive diagnosis of HCC while AFP increases%目的 探讨甲胎蛋白异质体(AFP-L3)的检测在预警原发性肝癌中的作用.方法 对100例AFP升高肝病患者血清,应用甲胎异质体微量离心柱分离并洗脱获得AFP-L3,再同时检测原始血清中的AFP以及AFP-L3含量,计算AFP-L3在AFP中的比例,对AFP-L3异常升高者、正常者进行跟踪,结合6个月后临床诊断结果 ,分析AFP-L3升高在鉴别良性肝脏病变与预警肝癌中的作用.结果 肝癌、疑似肝癌患者与良性肝病患者中的AFP-L3阳性率差异有统计学意义(分别为81.80%、73.68%、11.80%,P<0.05).未确诊肝癌(疑似HCC、肝病)的患者中,AFP-L3

  17. Characterization and enrichment of hepatic progenitor cells in adult rat liver

    Institute of Scientific and Technical Information of China (English)

    Ai-Lan Qin; Xia-Qiu Zhou; Wei Zhang; Hong Yu; Qin Xie

    2004-01-01

    AIM: To detect the markers of oval cells in adult rat liver and to enrich them for further analysis of characterization in vitro.METHODS: Rat model for hepatic oval cell proliferation was established with 2-acetylaminofluorene and two third partial hepatectomy (2-AAF/PH). Paraffin embedded rat liver sections from model (11 d after hepatectomy) and control groups were stained with HE and OV6, cytokeratin19 (CK19),albumin, alpha fetoprotein (AFP), connexin43, and c-kit antibodies by immunohistochemistry. Oval cell proliferation was measured with BrdU incorporation test. C-kit positive oval cells were enriched by using magnetic activated cell sorting (MACS) .The sorted oval cells were cultured in a low density to observe colony formation and to examine their characterization in vitroby immunocytochemistry and RT-PCR. RESULTS: A 2-AAF/PH model was successfully established to activate the oval cell compartment in rat liver. BrdU incorporation test of oval cell was positive. The hepatic oval cells coexpressed oval cell specific marker OV6, hepatocytemarker albumin and cholangiocyte-marker CK19. They also expressed AFP and connexin 43. C-kit, one hematopoietic stem cell receptor, was expressed in hepatic oval cells at high levels. By using c-kit antibody in conjunction with MACS,we developed a rapid oval cell isolation protocol. The sorted cells formed colony when cultured in vitro. Cells in the colony expressed albumin or CK19 or coexpressed both and BrdU incorporation test was positive. RT-PCR on colony showed expression of albumin and CK19 gene.CONCLUSION: Hepatic oval cells in the 2-AAF/PH model had the properties of hepatic stem/progenitor cells. Using MACS, we established a method to isolate oval cells. The sorted hepatic oval cells can form colony in vitro which expresses different combinations of phenotypic markers and genes from both hepatocytes and cholangiocyte lineage.

  18. Learn About Stem Cells

    Science.gov (United States)

    ... PDF) Download an introduction to stem cells and stem cell research. Stem Cell Glossary Stem cell terms to know. ... ISSCR Get Involved Media © 2015 International Society for Stem Cell Research Terms of Use Disclaimer Privacy Policy

  19. Stem Cell Basics

    Science.gov (United States)

    ... Information Stem Cell Basics Stem Cell Basics: Introduction Stem Cell Information General Information Clinical Trials Funding Information Current Research Policy Glossary Site Map Stem Cell Basics Introduction: What are stem cells, and why ...

  20. Changes of Serum Trace Elements, AFP, CEA, SF, T3, T4 and IGF- Ⅱ in Different Periods of Rat Liver Cancer

    Institute of Scientific and Technical Information of China (English)

    Hong-xu Zhang; Dan-dan Liu; Bai-jie Jin; Ya-wei Wang; Qj Liu; Ru-bing Duan; Peng Zhao; Ming-xia Ma

    2011-01-01

    Objective:Based on liver cancer model built in SD rats,the contents of trace elements (Cu,Fe,Zn,Ca and Mg),AFP,CEA,SF,TH and IGF-Ⅱ in serum were measured at different stages to explore the molecular changes during the rat liver cancer development.Methods:The SD rat liver cancer model was built by using diethylnitrosamine (DENA) as the mutagen.During 16 weeks of DENA gavage,blood samples were taken in the 14th,28th,56th,77th,105th and 112th days respectively after the first day of gavage with DENA,then the contents of five trace elements (Cu,Fe,Zn,Ca and Mg),T3,T4,IGF-Ⅱ,AFP,CEA and SF in serum were determined.Results:During the development of the rat liver cancer,in the test group,the Cu content significantly increased in serum,while the contents of Fe,Zn and Ca significantly decreased.The content of Mg showed no significant change.AFP and CEA of the test group showed same expression level with the control group; while the content of SF was lower than that of the control group when cancerization appeared.T3 and T4 increased at the first stage and then went down,and the content of IGF-Ⅱ was always high.Conclusion:Cu,Fe,Zn,Ca,T3,T4,SF and IGF-Ⅱ are closely related to the development of liver cancer.The changes of their contents in the development of cancer could enlighten the researches on cancer pathogenesis and prevention.

  1. Evaluation of tumor markers for the detection of hepatocellular carcinoma in Yangon General Hospital, Myanmar.

    Directory of Open Access Journals (Sweden)

    Taketa K

    2002-12-01

    Full Text Available Levels of alpha-fetoprotein (AFP, its glycoforms AFP-L3 and AFP-P4, and proteins induced by vitamin K absence or antagonist-II (PIVKA-II were determined in sera obtained from patients in Yangon General Hospital (20 with hepatocellular carcinoma (HCC, 29 with chronic liver diseases, including 3 with chronic hepatitis and 26 with cirrhosis of the liver, and 9 with other hepatobiliary diseases. Forty-five percent of the patients with HCC had serum AFP levels above 10,000 ng/ml, indicating that nearly half of the HCC patients were at an advanced stage of the disease. Thus, the AFP sensitivity was as high as 70% with 100% specificity for a cutoff level of 200 ng/ml. The sensitivity of AFP-L3 was 75% and a specificity 90% for a cutoff level of 15%. AFP-P4 showed a higher sensitivity of 80% and a similar specificity of 86% for a cutoff level of 12%. Combined evaluation of AFP-L3 and/or AFP-P4 increased the sensitivity to 90% with the same specificity of 86%, indicating that AFP-L3 and AFP-P4 are useful as adjuncts for diagnosis of HCC in the present population. PIVKA-II had a high sensitivity of 90%, although the specificity was lower than 45%, probably due to the low cutoff level, as some cholestatic patients were included in the control group.

  2. Liver-specific gene expression in mesenchymal stem cells is induced by liver cells

    Institute of Scientific and Technical Information of China (English)

    Claudia Lange; Philipp Bassler; Michael V. Lioznov; Helge Bruns; Dietrich Kluth; Axel R. Zander; Henning C. Fiegel

    2005-01-01

    AIM: The origin of putative liver cells from distinct bone marrow stem cells, e.g. hematopoietic stem cells or multipotent adult progenitor cells was found in recent in vitro studies. Cell culture experiments revealed a key role of growth factors for the induction of liver-specific genes in stem cell cultures. We investigated the potential of rat mesenchymal stem cells (MSC) from bone marrow to differentiate into hepatocytic cells in vitro. Furthermore,we assessed the influence of cocultured liver cells on induction of liver-specific gene expression.METHODS: Mesenchymal stem cells were marked with green fluorescent protein (GFP) by retroviral gene transduction. Clonal marked MSC were either cultured under liver stimulating conditions using fibronectin-coated culture dishes and medium supplemented with SCF, HGF,EGF, and FGF-4 alone, or in presence of freshly isolated rat liver cells. Cells in cocultures were harvested and GFP+ or GFP- cells were separated using fluorescence activated cell sorting. RT-PCR analysis for the stem cell marker Thy1 and the hepatocytic markers CK-18, albumin, CK-19,and AFP was performed in the different cell populations.RESULTS: Under the specified culture conditions, rat MSC cocultured with liver cells expressed albumin-, CK-18,CK-19, and AFP-RNA over 3 weeks, whereas MSC cultured alone did not show liver specific gene expression.CONCLUSION: The results indicate that (1) rat MSC from bone marrow can differentiate towards hepatocytic lineage in vitro, and (2) that the microenvironment plays a decisive role for the induction of hepatic differentiation of rMSC.

  3. Heterogeneity of human alpha-fetoprotein (HAFP) as revealed by agarose gel electrophoresis and isoelectric focusing in urea-acrylamide gels

    Energy Technology Data Exchange (ETDEWEB)

    Lester, E. P.; Miller, J. B.; Yachnin, S.

    1977-01-01

    HAFP was purified from five patients with hepatoma, one with gastric cancer, and one with an embryonal cell tumor, as well as from fetal liver and a monkey tumor cell line grown in tissue culture. The pattern of microheterogeneity of purified HAFP was defined for each HAFP isolate, and was demonstrated to be present in native sera, using crossed immunoelectrophoresis in agarose gels and isoelectric focusing in polyacrylamide gels containing 8 M urea. Three, and in one case four, species were seen in agarose, and were further resolved to reveal 6 major species with isoelectric focusing which could be correlated with the agarose gel variants. We have demonstrated a relationship between the immunosuppressive potency of certain HAFP preparations and the proportion of specific HAFP isomers which they contain as shown by these techniques. We have desialylated each of our preparations and demonstrated that this does not alter immunosuppressive potency but leaves residual complex microheterogeneity. Desialylated HAFP isolates contain six major HAFP isomers by isoelectric focusing, indicating that HAFP heterogeneity is based upon multiple charge differences in the HAFP molecule, apart from sialic acid content. The nature of these charge differences remain to be determined. We postulate that these charge differences modulate the immunosuppressive potency of HAFP.

  4. Circulating Osteopontin and Prediction of Hepatocellular Carcinoma Development in a Large European Population

    DEFF Research Database (Denmark)

    Duarte-Salles, Talita; Misra, Sandeep; Stepien, Magdalena;

    2016-01-01

    was conducted within the EPIC cohort. During a mean follow-up of 4.8 years, 100 HCC cases were identified. Each case was matched to two controls and OPN levels were measured in baseline plasma samples. Viral hepatitis, liver function and alpha-fetoprotein (AFP) tests were also conducted. Conditional...... subjects who developed HCC (AUC=0.86). For cases diagnosed within two years, the combination of OPN and AFP was best able to predict HCC risk (AUC=0.88). The best predictive model for HCC in this low-risk population is OPN in combination with liver function tests. Within two years of diagnosis, the...... combination of OPN and AFP best predicted HCC development, suggesting that measuring OPN and AFP could identify high-risk groups independently of a liver disease diagnosis....

  5. Stem Cells

    DEFF Research Database (Denmark)

    Sommerlund, Julie

    2004-01-01

    In his influential essay on markets, An essay on framing and overflowing (1998), Michel Callon writes that `the growing complexity of industrialized societies [is] due in large part to the movements of the technosciences, which are causing connections and interdependencies to proliferate'. This p...... and tantalizing than stem cells, in research, in medicine, or as products.......'. This paper is about tech-noscience, and about the proliferation of connections and interdependencies created by it.More specifically, the paper is about stem cells. Biotechnology in general has the power to capture the imagination. Within the field of biotechnology nothing seems more provocative...

  6. Why STEM?

    Science.gov (United States)

    Mitts, Charles R.

    2016-01-01

    The International Technology and Engineering Educators Association (ITEEA) defines STEM as a new transdisciplinary subject in schools that integrates the disciplines of science, technology, engineering, and mathematics into a single course of study. There are three major problems with this definition: There is no consensus in support of the ITEEA…

  7. Three independent techniques localize expression of transcript afp-11 and its bioactive peptide products to the paired AVK neurons in Ascaris suum: in situ hybridization, immunocytochemistry, and single cell mass spectrometry.

    Science.gov (United States)

    Jarecki, Jessica L; Viola, India R; Andersen, Kari M; Miller, Andrew H; Ramaker, Megan A; Vestling, Martha M; Stretton, Antony O

    2013-03-20

    We utilized three independent techniques, immunocytochemistry (ICC), single cell mass spectrometry (MS), and in situ hybridization (ISH), to localize neuropeptides and their transcripts in the nervous system of the nematode Ascaris suum . AF11 (SDIGISEPNFLRFa) is an endogenous peptide with potent paralytic effects on A. suum locomotory behavior. A highly specific antibody to AF11 showed robust immunostaining for AF11 in the paired AVK neurons in the ventral ganglion. We traced the processes from the AVK neurons into the ventral nerve cord and identified them as ventral cord interneurons. MS and MS/MS of single dissected AVKs detected AF11, two previously characterized peptides (AF25 and AF26), seven novel sequence-related peptides, including several sharing a PNFLRFamide C-terminus, and peptide NY, a peptide with an unrelated sequence. Also present in a subset of AVKs was AF2, a peptide encoded by the afp-4 transcript. By sequencing the afp-11 transcript, we discovered that it encodes AF11, all the AF11-related peptides detected by MS in AVK, and peptide NY. ISH detected the afp-11 transcript in AVK neurons, consistent with other techniques. ISH did not detect afp-11 in the ALA neuron, although both ICC and MS found AF11 in ca. 30% of ALAs. All 10 AF11-related peptides reduced acetylcholine-induced muscle contraction, but they differed in their rate of reversal of inhibition after removal of the peptide.

  8. Effect of paclitaxel liposome combined with nedaplatin on serum HE4, CA125, CA19-9, AFP, CEA and T lymphocyte subsets in patients with advanced ovarian cancer

    Institute of Scientific and Technical Information of China (English)

    Su-Yu Zhu; Jie Tan; Chen-Lu Zhang; Qun-Ying Wu; Xue-Xin Xie; Hua-Fang Yin

    2016-01-01

    Objective:To study the effect of paclitaxel liposome combined with nedaplatin on serum HE4, CA125, CA19-9, AFP, CEA and T lymphocyte subsets in patients with advanced ovarian cancer.Methods:A total of 80 patients with advanced ovarian cancer in our hospital from December 2012 to December 2015 were enrolled in this study. The subjects were divided into control group (n=40) and experiment group (n=40) randomly. The control group were treated with paclitaxel and cisplatin, the experiment group were treated with paclitaxel liposome combined with nedaplatin. 21 days for a period of treatment and the two groups were treated for 3 periods. The serum HE4, CA125, CA19-9, AFP, CEA levels and peripheral blood CD3+, CD4+, CD8+ and NK cells of the two groups before and after treatment were compared. Results:There were no significantly differences of the serum HE4, CA125, CA19-9, AFP, CEA level and peripheral blood CD3+, CD4+, CD8+ and NK cells of the two groups before treatment (P>0.05). The serum HE4, CA125, CA19-9, AFP and CEA level of the two groups after treatment were significantly lower than before treatment (P<0.05), and that of experiment were significantly lower than control group (P<0.05). The peripheral blood CD3+, CD4+, CD8+and NK cells of the two groups after treatment were significantly lower than before treatment (P<0.05), and that of experiment were significantly higher than control group (P<0.05). Conclusions:Paclitaxel liposome combined with nedaplatin can significantly reduce the serum HE4, CA125, CA19-9, AFP and CEA levels, improve peripheral blood CD3+, CD4+, CD8+ and NK levels of patients with advanced ovarian cancer, and it was worthy clinical application.

  9. Pluripotent stem cell lines

    OpenAIRE

    Yu, Junying; Thomson, James A.

    2008-01-01

    The derivation of human embryonic stem cells 10 years ago ignited an explosion of public interest in stem cells, yet this achievement depended on prior decades of research on mouse embryonic carcinoma cells and embryonic stem cells. In turn, the recent derivation of mouse and human induced pluripotent stem cells depended on the prior studies on mouse and human embryonic stem cells. Both human embryonic stem cells and induced pluripotent stem cells can self-renew indefinitely in vitro while ma...

  10. Safety and short-term efficacy of autologous bone marrow or umbilical cord blood stem cells transplantation through hepatic artery in treatment of patients with decompensated cirrhosis%经肝动脉自体骨髓或脐带血干细胞移植治疗失代偿期肝硬化患者的安全性和近期疗效比较

    Institute of Scientific and Technical Information of China (English)

    王方; 云升皓; 周新人; 寇俊峰; 蔡国芳; 陈瑞丽; 杨兴坤; 张静; 冯淑贤

    2014-01-01

    group (32 cases);patients in bone marrow group received autologous bone marrow stem cells transplantation through hepatic artery and the umbilicus cord blood group received umbilical cord blood stem cells through the same way;the changes of serum alanine aminotransferase,total bilirubin,prothrombin activity,albumin and alpha -fetoprotein after 8 weeks were determined,and the improvement in symptoms and postoperative adverse reactions were observed. Results Three days after cell transplantation,patients in both groups achieved significantly improvement in symptom such as fatigue and anorexia but had no significantly difference between the two groups. At eight weeks post therapy,in patients of bone marrow group and umbilicus group,serum albumin level increased significantly from the baseline of (31.0±4.6) g/L to (34.6±7.1)g/L and(26.6±8.0)g/L to(37.8±8.3)g/L,prothrombin activity from (48.8±13.4)% to (55.5±11.2)% and(47.5±12.5) to(58.9±14.0)%,but the improvement did not differ between the two groups;alanine aminotransferase,total bilirubin and alpha-fetoprotein were (45.6 ±12.3) IU/L,(28.1 ±13.5)μmol/L and (11.3 ±4.1)μg/L respectively in patients of bone marrow group and (47.2 ±11.8) IU/L,(30.7 ±14.8)μmol/L and (9.8 ±35) μg/L respectively in patients of umbilical group at 8 weeks post therapy,but the improvement did not differ between groups. Conclusions It is safe and effective in treatment of patients with decompensated cirrhosis using autologous bone marrow stem cells and umbilical cord blood stem cells transplantation through hepatic artery and no significant difference was observed between the two group.

  11. 罗氏2010免疫分析仪检测甲胎蛋白的性能验证%Analytical performance of roche 2010 immunoassay system in detecting AFP

    Institute of Scientific and Technical Information of China (English)

    王凡; 蒋红君

    2012-01-01

    目的 探讨罗氏2010免疫分析仪检测甲胎蛋白(AFP)的基本分析性能与质量要求的符合性.方法 参照相关的文件,分别对罗氏2010免疫分析仪检测AFP的精密度、准确度、临床可报告范围和参考区间进行验证.结果 测定均值为11.90 IU/ml的样本,验证后批内精密度为0.28 IU/ml,总精密度为0.45 IU/ml,测定均值为237.23 IU/ml的样本,验证后批内精密度为2.85 IU/ml,总精密度为3.50 IU/ml,均与厂家声明一致;高、低浓度定值质控品检测结果与靶值的相对偏差均小于5%,5份室间质评控制品检测结果与靶值的相对偏倚为-0.41%~6.61%;分析测量范围为0.514~992.7 IU/ml,临床可报告范围为0.514~158 832 IU/ml;参考区间与厂家声明一致.结论 罗氏2010电化学发光免疫分析仪测定AFP的精密度、准确度、分析测量范围性能均与厂家声明一致;此验证方案和实验方法简便易行,可用于其他检验项目的性能验证.%OBJECTIVE To validate the basic performance of Roche 2010 Immunoassay System in detecting fetoprotein (AFP). METHODS The precision, accuracy, reportable range and reference interval of the Roche 2012 Immunoassay System in detecting AFP were analyzed. RESULTS The results of within-run precisions were 0.28 IU/ml of 11.90 IU/ml AFP level and 2.85 IU/ml of 237.23 IU/ml AFP level. The total precisions were 0.45 IU/ml of 11.90 IU/ml AFP level and 3.50 IU/ml of 237.23 IU/ml AFP level. There results were consistent with manufacturer's instruction; The relative bias between the measured results and the target values was less than 5%, and the relative bias between the measured results and the EQA control samples at five levels was -0.41%-6.61%; measurement range was 0.514-992.7 IU/ml within manufacturer's liner range. Clinical re-portable range was 0.514-158 832 IU/ml; Reference interval was 0.0-5.8IU/ml, which was consistent with manufacturer's instruction. CONCLUSION The Roche 2010

  12. Evaluation of Individual and Combined Applications of Serum Biomarkers for Diagnosis of Hepatocellular Carcinoma: A Meta-Analysis

    Directory of Open Access Journals (Sweden)

    Bin Hu

    2013-12-01

    Full Text Available The clinical value of Serum alpha-fetoprotein (AFP to detect early hepatocellular carcinoma (HCC has been questioned due to its low sensitivity and specificity found in recent years. Other than AFP, several new serum biomarkers including the circulating AFP isoform AFP-L3, des-gamma-carboxy prothrombin (DCP and Golgi protein-73 (GP73 have been identified as useful HCC markers. In this investigation, we review the current knowledge about these HCC-related biomarkers, and sum up the results of our meta-analysis on studies that have addressed the utility of these biomarkers in early detection and prognostic prediction of HCC. A systematic search in PubMed, Web of Science, and the Cochrane Library was performed for articles published in English from 1999 to 2012, focusing on serum biomarkers for HCC detection. Data on sensitivity and specificity of tests were extracted from 40 articles that met the inclusion criteria, and the summary receiver operating characteristic curve (sROC was obtained. A meta-analysis was carried out in which the area under the curve (AUC for each biomarker or biomarker combinations (AFP, DCP, GP73, AFP-L3, AFP + DCP, AFP + AFP-L3, and AFP + GP73 was used to compare the diagnostic accuracy of different biomarker tests. The AUC of AFP, DCP, GP73, AFP-L3, AFP + DCP, AFP + AFP-L3, and AFP + GP73 are 0.835, 0.797, 0.914, 0.710, 0.874, 0.748, and 0.932 respectively. A combination of AFP + GP73 is superior to AFP in detecting HCC and differentiating HCC patients from non-HCC patients, and may prove to be a useful marker in the diagnosis and screening of HCC. In addition, the AUC of GP73, AFP + DCP and AFP + GP73 are better than that of AFP. The clinical value of GP73, AFP + DCP, or AFP + GP73 as serological markers for HCC diagnosis needs to be addressed further in future studies.

  13. Mixed ovarian germ cell tumor composed of immature teratoma, yolk sac tumor and embryonal carcinoma.

    Science.gov (United States)

    Wang, Ying; Zhou, Feng; Qian, Zhida; Qing, Jiale; Zhao, Mengdam; Huang, Lili

    2014-11-01

    We report the case of a 19-year-old woman experiencing lower abdominal distension and pain. Laboratory tests indicated elevated serum levels of Alpha-Fetoprotein (AFP) and human Chorionic Gonadotropin (hCG). A large mass was detected in the abdomen by physical examination and by transvaginal ultrasonography. Exploratory laparotomy was performed, and a smooth-surfaced, spherical, solid tumor was found on the left ovary, measuring 11.5 x 9.9 x 6.9 cm. Histological evaluation revealed that the tumor consisted of a combination of immature teratoma, Yolk Sac Tumor, and embryonal carcinoma; this is a very rare combination in mixed germ cell tumors. PMID:25518772

  14. Non-invasive monitoring of carcinogenesis in N-nitrosodiethylamine induced liver cancer

    Energy Technology Data Exchange (ETDEWEB)

    Park, Ju Hui; Kang, Joo Hyun; Lee, Yong Jin; Lee, Tae Sup; Kim, Kwang Il; Cheon, Gi Jeong; Choi, Chang Woon; Lim, Sang Moo [Korea Institute of Radiological and Medical Sciences, Seoul (Korea, Republic of); Park, Young Seo [Kyungwon University, Seongnam (Korea, Republic of)

    2010-10-15

    Molecular imaging based on reporter gene expression allows tissue-specific events or processes to be measured using the bioluminescence imaging (BLI) reporter gene expression vector controlled by specific enhancer/promoters. Alpha-fetoprotein (AFP), which is a tumor marker, is a serum glycoprotein that is expressed normally by fetal liver and yolk-sac cells, as well as in trace amounts in the fetal gastrointestinal tract. The serum concentration of AFP decreases rapidly after birth and its expression is repressed in adults. Approximately 80% of HCC patients show an increase in the AFP level. Therefore, AFP has been used for many years as a diagnostic and prognostic serum marker for HCC and transgenic system for AFP was proposed as a valuable tool for elucidation of mechanism of transcriptional regulation during liver development and hepatocarcinogenesis. In this study, firefly luciferase (fLuc) expressing transgenic mice controlled by the AFP enhancer/ promoter (enh/promoter) were produced to screen for the development of AFP-producing liver cancer. These models are expected to be useful for monitoring agents or drugs that modulate the AFP level as well as for measuring the specific signaling events important for liver cancer development

  15. Antioxidant and ACE-inhibitory activities of hemp (Cannabis sativa L.) protein hydrolysates produced by the proteases AFP, HT, Pro-G, actinidin and zingibain.

    Science.gov (United States)

    Teh, Sue-Siang; Bekhit, Alaa El-Din A; Carne, Alan; Birch, John

    2016-07-15

    Hemp protein isolates (HPIs) were hydrolysed by proteases (AFP, HT, ProG, actinidin and zingibain). The enzymatic hydrolysis of HPIs was evaluated through the degree of hydrolysis and SDS-PAGE profiles. The bioactive properties of the resultant hydrolysates (HPHs) were accessed through ORAC, DPPḢ scavenging and ACE-inhibitory activities. The physical properties of the resultant HPHs were evaluated for their particle sizes, zeta potential and surface hydrophobicity. HT had the highest rate of caseinolytic activity at the lowest concentration (0.1 mg mL(-1)) compared to other proteases that required concentration of 100 mg mL(-1) to achieve their maximum rate of caseinolytic activity. This led to the highest degree of hydrolysis of HPIs by HT in the SDS-PAGE profiles. Among all proteases and substrates, HT resulted in the highest bioactivities (ORAC, DPPḢ scavenging and ACE-inhibitory activities) generated from alkali extracted HPI in the shortest time (2 h) compared to the other protease preparations.

  16. Differentiation of bone mesenchymal stem cells into hepatocyte-like cells induced by liver tissue homogenate.

    Science.gov (United States)

    Xing, X K; Feng, H G; Yuan, Z Q

    2016-01-01

    This study investigated the efficacy and feasibility of inducing the differentiation of bone marrow-derived mesenchymal stem cells (BMSCs) into hepatocyte-like cells in vitro using Sprague Dawley rats, as a model of hepatocyte generation for cell transplantation. BMSCs were isolated and grown using the adherent method and exposed to 5 or 10% liver tissue homogenate, before being collected for analysis after 0, 7, 14, and 21 days. Immunofluorescence and western blotting were employed to detect the liver-specific markers a-fetoprotein (AFP) and albumin (ALB). Supernatant urea content was also measured to verify that differentiation had been induced. After 7 days in the presence of 10% liver tissue homogenate, BMSCs demonstrated hepatocyte-like morphological characteristics, and with prolonged culture time, liver-specific markers were gradually produced at levels indicating cell maturation. AFP expression peaked at 14 days then began to decrease, while both urea and ALB levels increased with induction time. Overall, marker expression in the 5% homogenate group was less than or equal to the 10% group at each time point. Thus, in a rat model, liver tissue homogenate obtained from partial hepatectomy can induce the differentiation of BMSCs into hepatocyte-like cells. This method is simple, feasible, and has remarkable real-world application potential. PMID:27525848

  17. 血清PIVKA-Ⅱ、AFP联合检测在肝癌诊断中的应用%Application of combined tests of serum PIVKA-Ⅱ and AFP in the diagnosis of liver cancer

    Institute of Scientific and Technical Information of China (English)

    沈聪龙; 郑丽萍

    2016-01-01

    目的 检测不同患者样本中拮抗剂Ⅱ诱导的蛋白(protein induced by vitamine K absence or antagonist-Ⅱ,PIVKA-Ⅱ)、甲胎蛋白(o-fetoprotein,AFP)的含量,研究PIVKA-Ⅱ、AFP在肝癌诊断中的应用.方法 选取我院2013年9月~2016年1月的住院患者血清样本共计259例,检测PIVKA-Ⅱ、AFP含量.结果 (1)在103例肝癌标本中,PIVKA-Ⅱ的阳性率为70.9%,AFP的阳性率41.7%,经过x2检验,差异有统计学意义(x2=17.76,P<0.01);(2)血清PIVKA-Ⅱ(≥40 mAu/mL)检测肝癌的灵敏度为70.2%;血清AFP(≥10 ng/mL)检测肝癌的灵敏度为69.9%.联合血清PIVKA-Ⅱ和AFP检测肝癌的灵敏度84.5%,较单独PIVKA-Ⅱ(x2=10.67,P<0.05)或单独AFP(x2=12.11,P<0.05)检测均有显著差异.结论 PIVKA-Ⅱ用于诊断肝癌灵敏度和特异性均优于AFP,可以显著提高对肝癌诊断的灵敏度和特异性.PIVKA-Ⅱ、AFP联合检测能更好更快地对肝癌做出早期诊断.

  18. Clinical Significance of Detection Combined AFP, AFU and CA199 to Patients with Primary Liver Cancer%原发性肝癌患者血清AFP、AFU、CA199联合检测的临床意义

    Institute of Scientific and Technical Information of China (English)

    毛莉萍; 徐建中

    2007-01-01

    目的 探讨原发性肝癌患者血清AFP、AFU、CA199含量及意义.方法 分别应用放射免疫法和生化法对46例原发性肝癌患者血清AFP、AFU、CA199含量进行检测,并与42名肝炎肝硬化患者和40名正常人进行比较.结果 原发性肝癌患者血清AFP、AFU、CA199含量显著高于肝炎肝硬化组及正常人组(P<0.01).AFP、AFU、CA199三项指标在原发性肝癌中的阳性率分别为76.09%、71.74%、80.43%,AFP+AFU、AFP+CA199、AFP+AFU+CA199联合检测的阳性率分别为89.13%、84.78%、95.65%.结论 联合检测血清AFP、AFU、CA199含量可以提高对原发性肝癌的诊断阳性率.

  19. Stem Cell Networks

    OpenAIRE

    Werner, Eric

    2016-01-01

    We present a general computational theory of stem cell networks and their developmental dynamics. Stem cell networks are special cases of developmental control networks. Our theory generates a natural classification of all possible stem cell networks based on their network architecture. Each stem cell network has a unique topology and semantics and developmental dynamics that result in distinct phenotypes. We show that the ideal growth dynamics of multicellular systems generated by stem cell ...

  20. Limbal stem cell transplantation

    OpenAIRE

    Fernandes Merle; Sangwan Virender; Rao Srinivas; Basti Surendra; Sridhar Mittanamalli; Bansal Aashish; Dua Harminder

    2004-01-01

    The past two decades have witnessed remarkable progress in limbal stem cell transplantation. In addition to harvesting stem cells from a cadaver or a live related donor, it is now possible to cultivate limbal stem cells in vitro and then transplant them onto the recipient bed. A clear understanding of the basic disease pathology and a correct assessment of the extent of stem cell deficiency are essential. A holistic approach towards management of limbal stem cell deficiency is needed. This ...

  1. Fish Stem Cell Cultures

    OpenAIRE

    Ni Hong, Zhendong Li, Yunhan Hong

    2011-01-01

    Stem cells have the potential for self-renewal and differentiation. First stem cell cultures were derived 30 years ago from early developing mouse embryos. These are pluripotent embryonic stem (ES) cells. Efforts towards ES cell derivation have been attempted in other mammalian and non-mammalian species. Work with stem cell culture in fish started 20 years ago. Laboratory fish species, in particular zebrafish and medaka, have been the focus of research towards stem cell cultures. Medaka is th...

  2. Stem Cell Separation Technologies

    OpenAIRE

    Zhu, Beili; Murthy, Shashi K

    2013-01-01

    Stem cell therapy and translational stem cell research require large-scale supply of stem cells at high purity and viability, thus leading to the development of stem cell separation technologies. This review covers key technologies being applied to stem cell separation, and also highlights exciting new approaches in this field. First, we will cover conventional separation methods that are commercially available and have been widely adapted. These methods include Fluorescence-activated cell so...

  3. Immune Response of Hepatocellular Carcinoma of in Patients with Different Stages of Liver Function%不同临床分期肝癌患者抗AFP Th1和Tc1应答反应研究

    Institute of Scientific and Technical Information of China (English)

    李林均; 余建云; 许涛; 张军; 程雄飞; 焦娇; 曹凤军; 姜孝新

    2015-01-01

    目的:研究CD4(Th1)和CD8(Tc1)T细胞对肝癌患者的AFP的应答反应及其与临床特征的相关性,为其早期诊断与预防提供新策略.方法:研究对象为62例HCC患者,30例肝硬化患者及30例健康志愿者;重点分析CD4T细胞和CD8T细胞对HCC患者的AFP-衍生肽的反应;用胞内细胞因子检测法对IFN-γ进行检测.结果:抗AFP的Tc1反应检测阳性结果在对照组为28.5%,在OkudaⅠ期的肝癌患者中为25.0%,在Ⅱ或Ⅲ期的HCC患者中为31.6%.抗AFP Th1阳性反应仅在HCC患者中检测到.抗AFP Th1阳性反应在44.4%的Child-Pugh A级的HCC患者中检测到,但是在Child-PughB或C级中仅15.4%.Tc1型反应在Child-PughA级肝功的患者中为16.7%,在Child-PughB或C级患者中为46.2%.结论:抗AFP Th1应答更多出现在早期肝硬化的HCC的患者中,而抗AFP Tc1应答更可能出现在晚期肝硬化患者中,这些结论为抗肝癌疫苗药物设计提供了理论基础.

  4. Radiofrequency ablation of AFP-positive patients with liver cancer%甲胎蛋白阳性肝癌病人的射频治疗

    Institute of Scientific and Technical Information of China (English)

    王克飞; 刘华顶; 武四化; 刘顺海; 冯丽

    2009-01-01

    目的:探讨甲胎蛋白(AFP)改变与肿瘤复发的关系,并观察肝癌射频消融(RFA)治疗前后AFP变化.方法:选AFP阳性的肝癌病人进行RFA治疗,术后6个月内不同时间段检测AFP改变及影像学变化,对AFP治疗前后阴转阳的病例实施再次RFA治疗或者介入治疗然后观察AFP变化,分析AFP改变与肿瘤复发的关系.结果:术后检查所有患者肿瘤血供不同程度减少、液化或肿瘤体积缩小:术后8~20天AFP检测阴性35例、降低6例、无变化8例,术后2、3、6个月AFP阴转阳率分别为2.85%、14.28%、20%,统计学处理P<0.05、P<0.01.治疗后AFP再次转阴.结论:AFP阳性肝癌病人RFA治疗后动态随访AFP阴转阳变化,对于了解术后肿瘤复发及评价肝癌RFA治疗效果有较大帮助.

  5. Transcription Factors and Medium Suitable for Initiating the Differentiation of Human-Induced Pluripotent Stem Cells to the Hepatocyte Lineage.

    Science.gov (United States)

    Tomizawa, Minoru; Shinozaki, Fuminobu; Motoyoshi, Yasufumi; Sugiyama, Takao; Yamamoto, Shigenori; Ishige, Naoki

    2016-09-01

    Transcription factors and culture media were investigated to determine the condition to initiate the differentiation of human-induced pluripotent stem (iPS) cells most efficiently. The expression of genes in human adult liver was compared with that in 201B7 cells (iPS cells) using cDNA microarray analysis. Episomal plasmids expressing transcription factors were constructed. 201B7 cells were transfected with the episomal plasmids and cultured in ReproFF (feeder-free media maintaining pluripotency), Leibovitz-15 (L15), William's E (WE), or Dulbecco's modified Eagle medium/Nutrient F-12 Ham (DF12) for 7 days. RNA was isolated and subjected to real-time quantitative PCR to analyze the expression of alpha-feto protein (AFP) and albumin. cDNA microarray analysis revealed 16 transcription factors that were upregulated in human adult liver relative to that in 201B7 cells. Episomal plasmids expressing these 16 genes were transfected into 201B7 cells. CCAAT/enhancer-binding protein alpha (CEBPA), CCAAT/enhancer-binding protein beta (CEBPB), forkhead box A1 (FOXA1), and forkhead box A3 (FOXA3) up-regulated AFP and down-regulated Nanog. These four genes were further analyzed. The expression of AFP and albumin was the highest in 201B7 cells transfected with the combination of CEBPA, CEBPB, FOXA1, and FOXA3 and cultured in WE. The combination of CEBPA, CEBPB, FOXA1, and FOXA3 was suitable for 201B7 cells to initiate differentiation to the hepatocyte lineage and WE was the most suitable medium for culture after transfection. J. Cell. Biochem. 117: 2001-2009, 2016. © 2016 Wiley Periodicals, Inc. PMID:26773721

  6. Residual dormant cancer stem-cell foci are responsible for tumor relapse after antiangiogenic metronomic therapy in hepatocellular carcinoma xenografts.

    Science.gov (United States)

    Martin-Padura, Ines; Marighetti, Paola; Agliano, Alice; Colombo, Federico; Larzabal, Leyre; Redrado, Miriam; Bleau, Anne-Marie; Prior, Celia; Bertolini, Francesco; Calvo, Alfonso

    2012-07-01

    Hepatocellular carcinoma (HCC) is the fifth most common solid tumor and the third leading cause of cancer-related deaths. Currently available chemotherapeutic options are not curative due in part to tumor resistance to conventional therapies. We generated orthotopic HCC mouse models in immunodeficient NOD/SCID/IL2rγ null mice by injection of human alpha-feto protein (hAFP)- and/or luciferase-expressing HCC cell lines and primary cells from patients, where tumor growth and spread can be accurately monitored in a non-invasive way. In this model, low-dose metronomic administration of cyclophosphamide (LDM-CTX) caused complete regression of the tumor mass. A significant increase in survival (P<0.0001), reduced aberrant angiogenesis and hyperproliferation, and decrease in the number of circulating tumor cells were found in LDM-CTX-treated animals, in comparison with untreated mice. Co-administration of LDM-CTX with anti-VEGF therapy further improved the therapeutic efficacy. However, the presence of residual circulating hAFP levels suggested that some tumor cells were still present in livers of treated mice. Immunohistochemistry revealed that those cells had a hAFP+/CD13+/PCNA- phenotype, suggesting that they were dormant cancer stem cells (CSC). Indeed, discontinuation of therapy resulted in tumor regrowth. Moreover, in-vitro LDM-CTX treatment reduced hepatosphere formation in both number and size, and the resulting spheres were enriched in CD13+ cells indicating that these cells were particularly resistant to therapy. Co-treatment of the CD13-targeting drug, bestatin, with LDM-CTX leads to slower tumor growth and a decreased tumor volume. Therefore, combining a CD13 inhibitor, which targets the CSC-like population, with LDM-CTX chemotherapy may be used to eradicate minimal residual disease and improve the treatment of liver cancer. PMID:22546866

  7. Diagnostic value of combined detection of serum AFU and AFP in predicting recurrence in patients with liver cancer%α-L岩藻糖苷酶和甲胎蛋白联合检测对肝癌患者术后复发的预测价值

    Institute of Scientific and Technical Information of China (English)

    梁红萍; 吉建民

    2010-01-01

    目的 探讨联合检测血清中α-L岩藻糖苷酶(AFU)和甲胎蛋白(AFP)在预测肝癌术后复发的价值.方法 按术后复发组、术后未复发组和健康对照组分3组检测血清AFU、AFP水平,AFU检测采用比色速率法,设定AFU>40U/L为阳性;AFP检测采用化学发光免疫法,设定AFP>13.6μg/L为阳性.结果 术后复发组与术后未复发组、健康对照组比较,AFU、AFP水平差异有统计学意义(P0.05).术后复发组肝癌复发前后AFU、AFP差异有统计学意义(P0.05);AFU+AFP、AFU、AFP三者特异度比较,差异无统计学意义(P>0.05).结论 联合检测血清中AFU、AFP水平,可显著提高肝癌术后复发的诊断率,并可作为预测肝癌术后复发的检测指标.%Objective To explore the value of the combined detection of serum AFU and AFP on predicting postoperative recurrence of hepatocellular carcinoma. Methods Three groups were classified as pathological observation group, pathological control group and health control group. AFU was detected by ratio colorimetric method with the AFU>40 U/L defined as positive values. AFP was detected by microparticle enzyme immunoassay with AFP> 13.6 μg/L defined as positive values. Results AFU and AFP level of the pathological observation group were significantly different compared with the pathological control group and health control group(P 0.05). For the pathological observation group, the level of AFU and AFP after recurrence were significantly different than that before (P 0.05). As to the specificity of method, there was no significant differences among AFU+AFP, AFP and AFU (P >0.05). Conclusion The combined detection of serum AFU and AFP levels significantly raised the diagnostic value of liver cancer recurrence. They may applied as a predictor or indicator of postoperative recurrence of hepatocellular carcinoma.

  8. 甲胎蛋白异质体3对肝细胞癌的预测作用——附18例分析

    Institute of Scientific and Technical Information of China (English)

    陈敏; 王永忠; 杭双荣; 申红玉; 濮翔科

    2009-01-01

    目的:探讨甲胎蛋白异质体3(lentil lectin-reactive alpha-fetoprotein-L3,AFP-L3)对肝癌的预测作用.方法:抽取18例经影像学(B超、CT等)诊断未发现肝癌的肝硬化患者的静脉血5 mL,采用国产微量离心柱法分离AFP-L3,用化学发光免疫法检测血标本中的甲胎蛋白和AFP-L3含量,以AFP-L3超过10%作为阳性判断标准.结果:18例肝硬化患者的AFP-L3分别为14%~75%,出院后分别接受3~18个月的随访,其中17例在出院后3~18个月内被诊断为肝癌.结论:AFP-L3阳性的肝硬化患者进展为肝癌的几率高,提示应对AFP-L3阳性的肝硬化患者进行密切随访,以便早期发现肝癌.

  9. Multiplexed electrochemical immunoassay of biomarkers using chitosan nanocomposites.

    Science.gov (United States)

    Chen, Xia; Ma, Zhanfang

    2014-05-15

    In this work, a novel and sensitive multiplexed immunoassay protocol for simultaneous electrochemical determination of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) was designed using functionalized chitosan composites. The immunosensing platform was prepared via immobilizing capture anti-AFP and anti-CEA on chitosan-Au nanoparticles (AuNPs) through EDC/NHS linking. The signal tags were fabricated by immobilizing electroactive redox probes - Prussian blue (PB) and ferrocenecarboxylic acid (Fc) on chitosan (CHIT), following by absorbing AuNPs to immobilize labeled anti-AFP and anti-CEA, respectively. A sandwich-type immunoassay format was employed for the simultaneous detection of AFP and CEA. The assay was based on the electrochemical oxidation/reduction of the redox species in signal tags, which has a relationship with the concentration of analytes. Experimental results revealed that the multiplexed electrochemical immunoassay enabled the simultaneous monitoring of AFP and CEA with a wide range of 0.05-100 ng mL(-1) for both AFP and CEA. The detection limits (LOD) was 0.03 ng mL(-1) for AFP and 0.02 ng mL(-1) for CEA (S/N=3). The assay results of serum samples with the proposed method were in a good agreement with the reference values from standard ELISA method. And the negligible cross-reactivity between the two analytes makes it possesses potential promise in clinical diagnosis. PMID:24413402

  10. 血清ALP、GGT、5’-NT与AFP联合检测在原发性肝癌诊断中的价值%Values of Combined Detection of Serum ALP, GGT, 5'-NT and AFP in the Diagnosis of Primary Hepatic Carcinoma

    Institute of Scientific and Technical Information of China (English)

    陈贞; 龙璐; 陶亚; 王堃; 易斌

    2012-01-01

    目的 探讨血清碱性磷酸酶(ALP)、谷氨酰转肽酶(GGT)、5核苷酸酶(5’-NT)以及AFP单独检测以及联合检测在原发性肝癌(PHC)诊断中的临床应用价值.方法 ALP、GGT、5’-NT检测采用酶速率法;AFP检测用电化学发光免疫分析法,分别检测原发性肝癌组(94例)、肝硬化组(60例)、慢性肝炎组(60例)、其他消化道肿瘤组(50例)和健康对照组(70例)血清ALP、GGT、5’-NT、AFP水平,应用ROC曲线对结果进行统计学分析.结果 AFP、ALP、GGT、5’-NT的ROC曲线下面积(AUCROC)分别为0.832、0.649、0.786、0.774; AFP、5’-NT血清含量PHC组与其他各组间差异有统计学意义(P<0.05).系列试验中,AFP+ GGT+5’-NT组合的各个特征参数指标较好;平行试验中,AFP/GGT/5’-NT的灵敏度超过了97%.结论 原发性肝癌诊断单项指标中以AFP的AUCROC最大,诊断价值最高;系列试验中以AFP+ GGT+5’-NT作为确诊PHC,平行试验中以AFP/GGT/5’-NT作为排除PHC较好的组合项目.%Objective To investigate the values of separate and combined detection of serum ALP, GGT, 5' - NT and AFP in the diagnosis of primary hepatic carcinoma (PHC). Methods Serum ALP, GGT and 5' — NT were detected by enzymatic rate method. Serum AFP was determined by electrochemical luminescence immunoassay. Sera from 94 patients with PHC, 60 patients with cirrhosis, 60 cases with chronic hepatitis, 50 with other gastrointestinal tumors and 70 healthy individuals were subject to AFP, ALP, GGT and 5' - NT detection. The results were analyzed with ROC curves. Results The areas under the ROC curve (AUCR0C)of AFP, ALP, GGT and 5' - NT were 0.832, 0.649, 0.786 and 0.774, respectively. Serum AFP and 5' — NT levels in the PHC group were statistically different from those in other groups (P<0.05). Ina series of tests, the combination of AFP , GGT and 5' - NT had fairly good characteristic parameters. In the parallel trial, the sensitivity of AFP/GGT/5' -NT exceeded

  11. Information on Stem Cell Research

    Science.gov (United States)

    ... Enhancing Diversity Find People About NINDS Information on Stem Cell Research Research @ NINDS Stem Cell Highlights Submit a hESC ... found here: Human Induced Pluripotent Stem Cells NINDS Stem Cell Research on Campus The Intramural Research Program of NINDS ...

  12. Vascular Endothelial Growth Factor Level as A Predictor of Hepatocellular Carcinoma in Liver Cirrhosis Patients

    Directory of Open Access Journals (Sweden)

    Benyamin Lukito

    2014-12-01

    Full Text Available BACKGROUND: Alpha-fetoprotein (AFP has been used for hepatocellular carcinoma (HCC diagnosis and screening, however, AFP has poor specificity. The extensive hypervascularity associated with HCC could be driven in part by the pro-angiogenic factor known as vascular endothelial growth factor (VEGF. Furthermore, invasiveness of certain HCC lesions has recently been linked to high levels of VEGF. Therefore, circulating VEGF levels of patients with liver cirrhosis (LC and HCC were investigated and analysed. METHODS: An analytical cross sectional study was designed. Diagnosis of HCC and LC was performed using clinical criteria and findings obtained from B-mode ultrasonography (USG, computed tomography (CT angiography, or magnetic resonance imaging (MRI. Blood were collected intravenously from all subjects. Obtained serum and plasma were stored in -80°C for following analyses: hepatitis B surface antigen (HBSAg, hepatitis C virus (HCV, alanine aminotransferase (ALT, total bilirubin, albumin, VEGF and AFP. RESULTS: Levels of VEGF and AFP were significantly higher in HCC group compared with LC group with p=3.05x10-6 and p=8.74x10-5, respectively. There was a significant positive correlation (p=0.029, r=0.309 between VEGF level and tumor size in HCC group. The area under curve (AUC for VEGF level in HCC and LC groups was 0.771. In the level of median 435.6 pg/mL VEGF, the sensitivity was 50% and specificity was 86%. In the level of 199.99 pg/mL VEGF the sensitivity was 74% and specificity was 76%. CONCLUSIONS: The present findings suggested that VEGF level could be a useful marker for the presence of HCC in patients with LC. KEYWORDS: hepatocellular carcinoma, HCC, liver cirrhosis, LC, vascular endothelial growth factor, VEGF, alpha-fetoprotein, AFP.

  13. Stem Cell Information: Glossary

    Science.gov (United States)

    ... Neurons Oligodendrocyte Parthenogenesis Passage Pluripotent Polar body Preimplantation Proliferation Regenerative medicine Reproductive cloning Signals Somatic cell Somatic cell nuclear transfer (SCNT) Somatic (adult) stem cell Stem cells Stromal cells Subculturing Surface markers ...

  14. What are Stem Cells?

    OpenAIRE

    Ahmadshah Farhat; Ashraf Mohammadzadeh; M. Rezaie

    2014-01-01

      Stem cells are undifferentiated self regenerating multi potential cells. There are three types of stem cells categories by the ability to form after cells and correlated with the body’s development process. Totipotent: these stem cells can form an entire organism such as fertilized egg. Ploripotent: ploripotent cells are those that can form any cell in the body but cannot form an entire organism such as developing embryo’s totipotent cells become ploripotent  Multipotent: Multi potent stem ...

  15. Optimizing stem cell culture.

    OpenAIRE

    van der Sanden, Boudewijn; Dhobb, Mehdi; Berger, François; Wion, Didier

    2010-01-01

    International audience Stem cells always balance between self-renewal and differentiation. Hence, stem cell culture parameters are critical and need to be continuously refined according to progress in our stem cell biology understanding and the latest technological developments. In the past few years, major efforts have been made to define more precisely the medium composition in which stem cells grow or differentiate. This led to the progressive replacement of ill-defined additives such a...

  16. Hepatic stem cell niches

    OpenAIRE

    Kordes, Claus; Häussinger, Dieter

    2013-01-01

    Stem cell niches are special microenvironments that maintain stem cells and control their behavior to ensure tissue homeostasis and regeneration throughout life. The liver has a high regenerative capacity that involves stem/progenitor cells when the proliferation of hepatocytes is impaired. In recent years progress has been made in the identification of potential hepatic stem cell niches. There is evidence that hepatic progenitor cells can originate from niches in the canals...

  17. Artificial Stem Cell Niches

    OpenAIRE

    Lutolf, Matthias P.; Blau, Helen M.

    2009-01-01

    Stem cells are characterized by their dual ability to reproduce themselves (self-renew) and specialize (differentiate), yielding a plethora of daughter cells that maintain and regenerate tissues. In contrast to their embryonic counterparts, adult stem cells retain their unique functions only if they are in intimate contact with an instructive microenvironment, termed stem cell niche. In these niches, stem cells integrate a complex array of molecular signals that, in concert with induced cell-...

  18. Intraoperative Stem Cell Therapy

    OpenAIRE

    Coelho, Mónica Beato; Cabral, Joaquim M. S.; Karp, Jeffrey M.

    2012-01-01

    Stem cells hold significant promise for regeneration of tissue defects and disease-modifying therapies. Although numerous promising stem cell approaches are advancing in clinical trials, intraoperative stem cell therapies offer more immediate hope by integrating an autologous cell source with a well-established surgical intervention in a single procedure. Herein, the major developments in intraoperative stem cell approaches, from in vivo models to clinical studies, are reviewed, and the poten...

  19. Epidemiological analysis of AFP cases of children in Lingshan County from 2008-2010%2008-2010年灵山县儿童急性弛缓性麻痹病例流行病学分析

    Institute of Scientific and Technical Information of China (English)

    劳成涌; 李齐芳; 邓肖英

    2012-01-01

    [Objective] To further improve acute flaccid paralysis (AFP) cases monitoring, and maintain polio-free status. [ Methods] Descriptive epidemiology was used to analyze the epidemiological characteristics of AFP cases and operating status of surveillance system in Lingshan County from 2008-2010. [Results] A total of 9, 8, 12 AFP cases were respectively reported in Lingshan County from 2008 -2010, with reported incidence rates of 2.58/100 000 and 2.59/100 000, and 3.94/100 000. The indexes of monitoring system reached the requirements. The qualified stool specimen collection rate was 96.42% (27/28), non-polio enteroviruses isolated in 6 cases, accounting for 20.68% , polio virus type isolated in 1 patient, accounting for 3.45% , excluded for spinal cord gray by the National Polio Laboratory. [ Conclusion ] Lingshan surveillance system of AFP cases is running well. Routine immunization, supplementary immunization, qualified AFP case specimen collection and reporting rate? improvement plays an important role in maintaining polio-free status.%目的 进一步做好急性弛缓性麻痹(AFP)病例的监测工作,巩固无脊髓灰质炎成果.方法 采用描述流行病学方法对灵山县2008-2010年AFP病例的流行病学特征和监测系统运转情况进行分析.结果 灵山县2008-2010年分别报告AFP病例9、8和12例,报告发病率分别为2.58/10万、2.59/10万、和3.94/10万,AFP病例监测系统各项指标均达要求,合格粪便标本采集率为96.42%(27/28),分离出非脊髓灰质炎肠道病毒6例,占20.68%;分离出脊髓灰质炎病毒Ⅰ型1例,占3.45%,经国家脊髓灰质炎实验室鉴定后排除.结论 灵山县儿童AFP病例监测系统运转情况良好.做好常规免疫、强化免疫、查漏补种和AFP病例合格标本的采集工作,提高首诊报告率,是有效维持无脊髓灰质炎状态的重要工作.

  20. Upregulation of MiR-122 via Trichostatin A Treatments in Hepatocyte-like Cells Derived from Mesenchymal Stem Cells.

    Science.gov (United States)

    Alizadeh, Effat; Eslaminejad, MohamadReza Baghaban; Akbarzadeh, Abolfazl; Sadeghi, Zohre; Abasi, Mozghan; Herizchi, Roya; Zarghami, Nosratollah

    2016-02-01

    The miR-122 is a tissue-specific miRNA; its expression is abundant in liver. MiR-122 upregulation is crucial for differentiation, functionality, and maintenance of differentiated phenotype in hepatocytes. The improving effects of trichostatin A (TSA) on hepatic differentiation have been reported previously. The aim of this study was to determine whether TSA can affect the expression of miR-122 in hepatocyte-like cells (HLCs) generated from human adipose tissue-derived mesenchymal stem cells (hAT-MSCs). The hepatic differentiation of hAT-MSCs induced by a mixture of growth factors and cytokines either with or without TSA treatments. The functionality of HLCs generated with or without TSA and the expression levels of miR-122 were studied. The expression levels of miR-122 in TSA-treated HLCs was significantly (p < 0.05) higher than those generated by growth factors and cytokines, only. The downregulation of a-fetoprotein (AFP) levels but enhanced albumin synthesis (p < 0.05) and upregulation of liver-enriched transcription factors (LETFs) HNF4α (hepatocyte nuclear factor 4α) and HNF6 (hepatocyte nuclear factor 6) were observed in TSA-treated HLCs (p < 0.05). In conclusion, administration of TSA in hepatogenic differentiation of hAT-MSCs resulted in higher expression levels of miR-122, facilitation of differentiation, and subsequently attenuation of AFP levels.

  1. Umbilical cord-derived mesenchymal stem cells inhibit growth and promote apoptosis of HepG2 cells.

    Science.gov (United States)

    Tang, Ying-Mei; Bao, Wei-Min; Yang, Jin-Hui; Ma, Lin-Kun; Yang, Jing; Xu, Ying; Yang, Li-Hong; Sha, Feng; Xu, Zhi-Yuan; Wu, Hua-Mei; Zhou, Wei; Li, Yan; Li, Yu-Hua

    2016-09-01

    Hepatocellular carcinoma is the fifth most common type of cancer worldwide and remains difficult to treat. The aim of this study was to investigate the effects of mesenchymal stem cells (MSCs) derived from the umbilical cord (UC‑MSCs) on HepG2 hepatocellular carcinoma cells. UC‑MSCs were co‑cultured with HepG2 cells and biomarkers of UC‑MSCs were analyzed by flow cytometry. mRNA and protein expression of genes were determined by reverse transcription‑polymerase chain reaction and flow cytometry, respectively. Passage three and seven UC‑MSCs expressed CD29, CD44, CD90 and CD105, whereas CD34 and CD45 were absent on these cells. Co‑culture with UC‑MSCs inhibited proliferation and promoted apoptosis of HepG2 cells in a time‑dependent manner. The initial seeding density of UC‑MSCs also influenced the proliferation and apoptosis of HepG2 cells, with an increased number of UC‑MSCs causing enhanced proliferation inhibition and cell apoptosis. Co‑culture with UC‑MSCs downregulated mRNA and protein expression of α‑fetoprotein (AFP), Bcl‑2 and Survivin in HepG2 cells. Thus, UC‑MSCs may inhibit growth and promote apoptosis of HepG2 cells through downregulation of AFP, Bcl‑2 and Survivin. US-MSCs may be used as a novel therapy for treating hepatocellular carcinoma in the future. PMID:27485485

  2. ROLE OF ALPHA FETO PROTIEN AS A MARKER IN DIAGNOSIS OF PREMATURE RUPTURE OF MEMBRANES IN RURAL POPULATION

    Directory of Open Access Journals (Sweden)

    Pahula

    2015-08-01

    Full Text Available AIM : In India, especially in rural population multiple factors adversely affect the incidence of premature rupture of membrane. The purpose of this study is to test the amniotic fluid Alpha feto protein as a reliable mar ker for the detection of PPROM and to test its efficacy for the purpose in the rural India. STUDY DESIGN : This study was conducted in the Department of Obstetrics & Gynecology, NIMS Medical College & Hospital, Jaipur, Rajasthan. This was a cross sectional study during period of one year with sample size of 100 patients . METHOD S : Patients who were at ≥24 weeks of gestation with the complains of leaking per vaginum. Sample for Alpha - fetoprotein (AFP estimation was collected by instilling 5 ml of distilled wa ter into vagina, irrespective of pooling of amniotic fluid and sent to the biochemistry lab for the analysis and for the estimation of alpha fetoprotein by using Electroluminescence enzyme immunoassay method. RESULT: The sensitivity, specificity, positive predictive value and negative predictive value are 92%, 98%, 92% and 98% respectively. CONCLUSION: The AFP can be used as reliable marker to avoid unnecessary obstretic interventions for diagnosis of PROM.

  3. 异常凝血酶原和肝细胞癌%Des-gamma-carboxy-prothrombin and Hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    袁联文; 唐伟; 幕内·雅敏

    2002-01-01

    目前肝细胞癌(hepatocellular carcinoma,HCC)的诊断主要有影像学诊断和血清肿瘤标志物的检测.异常凝血酶原(des-gamma-carboxy-prothrombin,DCP)又被称为PIVKA-Ⅱ(protein induced by vita-min K absence or antagonist-Ⅱ),与AFP(alpha-fetoprotein)和AFP-L3(alpha-fetoprotein L3 fraction)一样被认为是一种很有价值的肝细胞癌血清肿瘤标志物.在HCC的检测诊断上,它们之间无明显相关关系,而表现为一定的互补性,结合影像学诊断,动态观测HCC高危(肝炎、肝硬化)人群,这些血清肿瘤标志物有助于HCC的早期发现,同时对HCC的手术疗效的评价、预后的估评有着一定的指导意义.

  4. 急性弛缓性麻痹病例现场流行病学监测与质量评价%Field Epidemiological Surveillance and Quality Assessment for Acute Flaccid Paralysis (AFP) Cases

    Institute of Scientific and Technical Information of China (English)

    梅志强; 张俊书; 范富云; 徐健

    2001-01-01

    AFP case reporting and epidemiological survey are the main contents of field epidemiological surveillance, also are the basis and premise for assessment of sensitivity and specificity of AFP case surveillance system. AFP case reported rates were>1/100,000 in our province since 1995 and was 1.51/100,000 in 1999. Five indicators for field surveillance met or exceeded the state-established lower limit indicator of 80% since 1996 and was>96.0% in 1999. Of 697 AFP cases reported in 1993~1999, 8 kinds of diseases were classified, in which non-polio enterovirus took the first place (43.33%), Guillan-Barre syndrome came the second (27.12%). The last polio case confirmed virologically occurred on April 4, 1992 which was the last wild poliovirus case since supplementary immunization campaigns have been performed in our province.%急性弛缓性麻痹(AFP)病例报告和流行病学调查,是现场流行病学监测的主要内容,是评价AFP病例监测敏感性和特异性的基础和前提。据此,对山西省1993~1999年AFP病例监测的质量进行了评估。山西省AFP病例报告发病率从1995年起已>1/10万,1999年为1.51/10万;现场监测的5项指标,从1996年起都达到了规定的要求,1999年都>96.0%。最后1例病毒学确诊的脊髓灰质炎(脊灰)病例发生于1992年4月。1993~1999年697例AFP病例,临床诊断以非脊灰肠道病毒感染最多,占43.33%;其次为格林-巴利综合征,占27.12%。表明山西省AFP病例监测的质量是好的。

  5. 肿瘤标志物DCP和AFP在原发性肝癌中的诊断价值%Utility of DCP and AFP in diagnosis of primary liver cancer

    Institute of Scientific and Technical Information of China (English)

    郑海伦; 赵睿; 李大鹏; 汪强武; 汪建超; 王启之

    2016-01-01

    目的 探讨血清肿瘤标志物异常凝血酶原(DCP)和甲胎蛋白(AFP)单独或联合检测在原发性肝癌(PLC)诊断中的临床意义.方法 采用酶联免疫吸附测定法(ELISA)分别对70例PLC患者、每组各50例的肝硬化患者、乙型肝炎患者、健康体检者的血清DCP和AFP进行检测,并比较各组水平差异,并采用Spearman相关分析探讨AFP和DCP之间的关系,用ROC曲线评价AFP和DCP诊断PLC的敏感度、特异度,从而明确DCP和AFP在PLC中的诊断价值.结果 PLC组患者血清中的DCP和AFP水平均显著高于肝硬化、肝炎和正常组,差异具有统计学意义(P<0.01).单独检测诊断PLC时,通过ROC曲线分析DCP的曲线下面积大于AFP(P <0.01),分别为0.977(95% CI:0.954 ~1.000)和0.853 (95% CI:0.785~0.921),Spearman相关分析表明AFP和DCP之间无显著相关性.单独检测时DCP诊断PLC的敏感度(90.0%)和特异度(97.3%)显著高于AFP(77.1%,76.7%),AFP和DCP联合检测时可提高诊断PLC的敏感度(95.7%).结论 DCP是诊断PLC良好的血清肿瘤标志物,诊断PLC时的敏感度和特异度高于AFP;联合检测DCP和AFP可提高PLC诊断的敏感度,避免临床漏诊PLC的发生.

  6. Plant stem cell niches.

    Science.gov (United States)

    Aichinger, Ernst; Kornet, Noortje; Friedrich, Thomas; Laux, Thomas

    2012-01-01

    Multicellular organisms possess pluripotent stem cells to form new organs, replenish the daily loss of cells, or regenerate organs after injury. Stem cells are maintained in specific environments, the stem cell niches, that provide signals to block differentiation. In plants, stem cell niches are situated in the shoot, root, and vascular meristems-self-perpetuating units of organ formation. Plants' lifelong activity-which, as in the case of trees, can extend over more than a thousand years-requires that a robust regulatory network keep the balance between pluripotent stem cells and differentiating descendants. In this review, we focus on current models in plant stem cell research elaborated during the past two decades, mainly in the model plant Arabidopsis thaliana. We address the roles of mobile signals on transcriptional modules involved in balancing cell fates. In addition, we discuss shared features of and differences between the distinct stem cell niches of Arabidopsis.

  7. Toward 'SMART' stem cells.

    Science.gov (United States)

    Cheng, T

    2008-01-01

    Stem cell research is at the heart of regenerative medicine, which holds great promise for the treatment of many devastating disorders. However, in addition to hurdles posed by well-publicized ethical issues, this emerging field presents many biological challenges. What is a stem cell? How are embryonic stem cells different from adult stem cells? What are the physiological bases for therapeutically acceptable stem cells? In this editorial review, I will briefly discuss these superficially simple but actually rather complex issues that surround this fascinating cell type. The goal of this special issue on stem cells in Gene Therapy is to review some fundamental and critical aspects of current stem cell research that have translational potential. PMID:18046429

  8. Lung Cancer Stem Cells

    Directory of Open Access Journals (Sweden)

    Sharon R. Pine

    2008-01-01

    Full Text Available Lung cancer remains a major cause of cancer-related lethality because of high incidence and recurrence in spite of significant advances in staging and therapies. Recent data indicates that stem cells situated throughout the airways may initiate cancer formation. These putative stem cells maintain protumorigenic characteristics including high proliferative capacity, multipotent differentiation, drug resistance and long lifespan relative to other cells. Stem cell signaling and differentiation pathways are maintained within distinct cancer types, and destabilization of this machinery may participate in maintenance of cancer stem cells. Characterization of lung cancer stem cells is an area of active research and is critical for developing novel therapies. This review summarizes the current knowledge on stem cell signaling pathways and cell markers used to identify the lung cancer stem cells.

  9. Quantitative Proteomics Analysis of Tissue Interstitial Fluid for Identification of Novel Serum Candidate Diagnostic Marker for Hepatocellular Carcinoma.

    Science.gov (United States)

    Sun, Wei; Xing, Baocai; Guo, Lihai; Liu, Zhilei; Mu, Jinsong; Sun, Longqin; Wei, Handong; Zhao, Xiaohang; Qian, Xiaohong; Jiang, Ying; He, Fuchu

    2016-01-01

    Hepatocellular carcinoma (HCC) is the fifth most common malignant cancer in the world. The sensitivity of alpha-fetoprotein (AFP) is still inadequate for HCC diagnosis. Tissue interstitial fluid (TIF), as the liquid microenvironment of cancer cells, was used for biomarker discovery in this study. Paired tumor and nontumor TIF samples from 6 HBV-HCC patients were analyzed by a proteomic technique named iTRAQ (isobaric tag for relative and absolute quantitation). Totally, 241 up-regulated proteins (ratio ≥ 1.3, p AFP) and specificity of 66%. This result demonstrated the potential of S100A9 as a candidate HCC diagnostic biomarker. And TIF was a kind of promising material to identify candidate tumor biomarkers that could be detected in serum. PMID:27216119

  10. [Complete Resection of Non-seminomatous Germ Cell Tumor with Plastron Approach].

    Science.gov (United States)

    Suzuki, Jun; Oizumi, Hiroyuki; Kato, Hirohisa; Endoh, Makoto; Watarai, Hikaru; Hamada, Akira; Suzuki, Katsuyuki; Nakahashi, Kenta; Sasage, Takayuki; Sadahiro, Mitsuaki

    2016-07-01

    A 17-year-old man was admitted to our hospital for the abnormal chest shadow. Chest computed tomography(CT) demonstrated mediastinal tumor, measuring 13 cm in diameter with high serum level of alpha fetoprotein (AFP) and human chorionic gonadotropin (hCG). The lesions were diagnosed as mixed germ cell tumors including a non-seminomatous malignant component by CT guided needle biopsy. After 5 courses of chemotherapy, the serum AFP and hCG were decreased almost normal level but the tumor size was not changed. Because it seemed to be difficult to get sufficient operating field with standard median sternotomy and patient wanted to treat funnel chest, we selected tumor resection with plastron approach. The tumor was completely resected with a good operation field by this procedure. PMID:27365059

  11. The 12th quality control survey for radioisotope in vitro tests in Japan, 1990

    Energy Technology Data Exchange (ETDEWEB)

    1991-10-01

    The results of the 12th quality control nationwide survey is presented. Of 670 selected facilities, 405 (60.4%) participated in this survey. Myoglobin and trypsin were added as new items to be examined. The other conventional items were as follows: alpha-fetoprotein (AFP), aldosterone, {beta}{sub 2}-microglobulin, carbohydrate angigen 15-3, C-peptide, digoxin, elastase 1, free triiodothyronine, growth hormone, immunoglobulin E, prostatic acid phosphatase, pancreatic secretory trypsin inhibitor, progesterone, prolactin, thyroglobulin, triiodothyronine (T{sub 3}), T{sub 3} uptake, tissue polypeptide antigen, thyroid stimulating hormone, and testosterone. There was a great coefficient of variation (CV) between kits in AFP, aldosterone, progesterone, and prolactin. These results were analogous to those in the previous surveys. For T{sub 3}, there was a great difference between CV by radioimmunoassay and by non-isotopic method. Both myoglobin and trypsin had a great difference between kits. (N.K.).

  12. An Overview of Biomarkers and Molecular Signatures in HCC

    Directory of Open Access Journals (Sweden)

    Seon-Hee Yim

    2010-05-01

    Full Text Available Hepatocellular carcinoma (HCC is the third most common cause of cancer mortality worldwide. Although most HCCs seem to originate from the accumulation of genetic abnormalities induced by various risk factors, underlying mechanisms of hepatocarcinogenesis remain unclear. Long-term survival of HCC patients is also poor, partly due to HCC recurrence. Although serum alpha-fetoprotein (AFP level is a useful marker for the detection and monitoring of HCC, AFP levels may remain normal in the patients even with advanced HCC. To identify useful biomarkers for HCC, many studies have been conducted on molecular events such as genetic and epigenetic alterations, and gene expression. This review summarizes recent studies of potential molecular markers for diagnosis and monitoring metastasis or recurrence of HCC.

  13. An Overview of Biomarkers and Molecular Signatures in HCC

    Energy Technology Data Exchange (ETDEWEB)

    Yim, Seon-Hee [Integrated Research Center for Genome Polymorphism, The Catholic University of Korea School of Medicine, 505 Banpo-dong, Seocho-gu, Seoul 137-701 (Korea, Republic of); Chung, Yeun-Jun, E-mail: yejun@catholic.ac.kr [Integrated Research Center for Genome Polymorphism, The Catholic University of Korea School of Medicine, 505 Banpo-dong, Seocho-gu, Seoul 137-701 (Korea, Republic of); Department of Microbiology, The Catholic University of Korea School of Medicine, 505 Banpo-dong, Seocho-gu, Seoul 137-701 (Korea, Republic of)

    2010-05-07

    Hepatocellular carcinoma (HCC) is the third most common cause of cancer mortality worldwide. Although most HCCs seem to originate from the accumulation of genetic abnormalities induced by various risk factors, underlying mechanisms of hepatocarcinogenesis remain unclear. Long-term survival of HCC patients is also poor, partly due to HCC recurrence. Although serum alpha-fetoprotein (AFP) level is a useful marker for the detection and monitoring of HCC, AFP levels may remain normal in the patients even with advanced HCC. To identify useful biomarkers for HCC, many studies have been conducted on molecular events such as genetic and epigenetic alterations, and gene expression. This review summarizes recent studies of potential molecular markers for diagnosis and monitoring metastasis or recurrence of HCC.

  14. Liver Rapid Reference Set Application: Hemken - Abbott (2015) — EDRN Public Portal

    Science.gov (United States)

    The aim for this testing is to find a small panel of biomarkers (n=2-5) that can be tested on the Abbott ARCHITECT automated immunoassay platform for the early detection of hepatocellular carcinoma (HCC). This panel of biomarkers should perform significantly better than alpha-fetoprotein (AFP) alone based on multivariate statistical analysis. This testing of the EDRN reference set will help expedite the selection of a small panel of ARCHITECT biomarkers for the early detection of HCC. The panel of ARCHITECT biomarkers Abbott plans to test include: AFP, protein induced by vitamin K absence or antagonist-II (PIVKA-II), golgi protein 73 (GP73), hepatocellular growth factor (HGF), dipeptidyl peptidase 4 (DPP4) and DPP4/seprase (surface expressed protease) heterodimer hybrid. PIVKA-II is abnormal des-carboxylated prothrombin (DCP) present in vitamin K deficiency.

  15. Regression of hepatocellular carcinoma during vitamin K administration

    Institute of Scientific and Technical Information of China (English)

    Kazuhiro Nouso; Nobuaki Okano; Masahiro Nakagawa; Motowo Mizuno; Yasuyuki Araki; Yasushi Shiratori; Shuji Uematsu; Kunihiro Shiraga; Ryoichi Okamoto; Ryo Harada; Shoko Takayama; Wakako Kawai; Shigeru Kimura; Toru Ueki

    2005-01-01

    An 85-year-old man with HCV infection and diabetes mellitus was diagnosed as having hepatocellular carcinoma (HCC, 13 cm in diameter) based on high serum alpha-fetoprotein (AFP),AFP-L3,and des-γ-carboxy prothrombin levels as well as typical enhancement pattern on contrast-enhanced CT. The patient did not receive any interventional treatments because of advanced age and the advanced stage of HCC.He chose to take vitamin K,which was reported to suppress the growth of HCC in vitro. Three months after starting vitamin K, all three tumor markers were normalized and HCC was markedly regressed, showing no enhancement in the early arterial phase on CT. Here we present the report describing the regression of HCC during the administration of vitamin K.

  16. 腺病毒介导蜂毒素基因转染对HepG2细胞生长及AFP表达的影响%Effect of recombinant adenovirus harboring melittin gene on growth of HepG2 cells and AFP expression

    Institute of Scientific and Technical Information of China (English)

    李绍祥; 文军慧; 凌昌全

    2004-01-01

    目的:将蜂毒素基因置于甲胎蛋白(AFP)转录调控元件(rAFP)驱动之下,构件重组腺病毒载体,观察蜂毒素基因转染对肝癌细胞生长及AFP表达的影响.方法::将蜂毒素基因置于rAFP之后,用细菌内高效同源重组法将目的基因重组入腺病毒质粒中,将腺病毒质粒用PacⅠ酶切线性化后,用脂质体介导转染293细胞进行腺病毒的包装.携有蜂毒素基因的腺病毒感染肝癌细胞后,RT-PCR实验观察蜂毒素基因是否发生转录;MTT法测定蜂毒素基因转染对肝癌细胞增殖的影响;ELISA双抗体夹心法定量检测AFP.结果:RT-PCR实验表明蜂毒素基因转染肝癌细胞后可以被转录;蜂毒素基因在rAFP的控制下,可以特异性的抑制AFP阳性肝癌细胞的增殖,并降低其AFP的生成量.结论:蜂毒素基因转染对肝癌细胞的生长及AFP的表达具有抑制作用,可降低其恶性度.%Objective:To construct recombinant adenovirus harboring melittin gene under the control of AFP transcription regulatory element rAFP,and to observe its tumor inhibition effects in vitro as well as its effect on AFP expression in hepatocarcinoma cells.Methods:Melittin gene was driven by rAFP.The gene of interest was cloned into adenoviral backbone plasmid through a new simplified bacterial homologous recombinant system,then the recombinant adenoviral plasmids were linearized with PacⅠand then were used to transfect 293 cells (mediated by lipofectin) for generation of the desired recombinant adenoviruses.The resultant viruses harboring melittin gene was used to infect the AFP-positive HepG2 cells. Transcription of melittin gene was verified by RT-PCR,and its proliferation inhibition effect was observed by MTT assay. AFP was measured by ELISA double antibody sandwiched method.Results:Recombinant adenoviruses harboring melittin gene were successfully constructed and transfect hepatocarcinoma cells,and melittin gene was transcripted.Under the control of rAFP

  17. Microchip-based ELISA strategy for the detection of low-level disease biomarker in serum

    Energy Technology Data Exchange (ETDEWEB)

    Liu Yun; Wang Huixiang; Huang Jingyu; Yang Jie; Liu Baohong [Department of Chemistry, Institute of Biomedical Sciences, Fudan University, Shanghai 200433 (China); Yang Pengyuan, E-mail: pyyang@fudan.edu.cn [Department of Chemistry, Institute of Biomedical Sciences, Fudan University, Shanghai 200433 (China)

    2009-09-14

    A simple and sensitive method has been proposed to determine a trace level of {alpha}-fetoprotein (AFP), hepatocellular carcinoma biomarker, using poly(methyl methacrylate) (PMMA) microfluidic chips coupled with electrochemical detection system. The PMMA microchannels have been modified with poly(ethyleneimine) (PEI) containing abundant NH{sub 2} groups to covalently immobilize AFP monoclonal antibody. Afterward, the antigen AFP and horseradish peroxidase (HRP)-conjugated AFP antibody can sequentially bind through antigen-antibody specific interaction. Atomic force microscopy (AFM) and confocal fluorescence microscope (CFFM) were utilized to characterize the surface topography and protein immobilization after modification. Coupled with three-electrode electrochemical detection system, the immunochip can perform the detection limit of AFP down to 1 pg mL{sup -1}, and achieve a detectable linear concentration range of 1-500 pg mL{sup -1} by differential pulse voltammetry (DPV). The on-chip immunoassay platform can not only provide rapid and sensitive detection for target proteins but also be resistant to non-specific adsorption of proteins, which contributes to the detection of low-level protein in real sample. Finally, AFP existing in healthy human serum was detected to demonstrate the utility of the immunochip. The result shows that the proposed approach is feasible and has the potential application in clinical analysis and diagnosis.

  18. Morphological Changes of Hepatocarcinoma Cell Line HepG2 Transfected by Recombinant Adenovirus Carrying Melittin Gene and α-fetoprotein Promotor%含rAFP的蜂毒素基因重组腺病毒转染的HepG2细胞形态学变化

    Institute of Scientific and Technical Information of China (English)

    陈永安; 张亚妮; 顾伟; 李柏; 董惠娟; 凌昌全

    2010-01-01

    目的 通过构建含有重组AFP(recombinant alpha-fetoprotein,rAFP)启动子的携蜂毒素(melittin,Mel)基因的重组腺病毒(adenovirus,Ad),观察感染Ad-rAFP-Mel的HepG2细胞形态学变化.方法 采用倒置显微镜、苏木精-伊红(hematoxylin-eosin,HE)染色、Hoechst 33258染色、透射电镜观察Ad-rAFP-Mel作用过的肝癌细胞死亡情况、细胞器的结构变化等形态学变化.结果 倒置显微镜下观察结果显示,随着病毒感染复数的增加和时间的延长,活细胞数量减少;HE染色显示坏死与凋亡并存;Hoechst 33258 染色显示确有凋亡存在;透射电镜证实Ad-rAFP-Mel作用于肝癌细胞后,肝癌细胞凋亡和坏死兼而有之.结论 Ad-rAFP-Mel能够抑制 HepG2 细胞的生长,细胞死亡呈现坏死与凋亡兼有的特征.

  19. Combined treatment of vitamin K2 and angiotensin-converting enzyme inhibitor ameliorates hepatic dysplastic nodule in a patient with liver cirrhosis

    Institute of Scientific and Technical Information of China (English)

    Hitoshi Yoshiji; Kosuke Kaji; Masahito Uemura; Junichi Yamao; Masao Fujimoto; Akira Mitoro; Masahisa Toyohara; Motoyuki Yoshida; Hiroshi Fukui; Ryuichi Noguchi; Masaharu Yamazaki; Yasuhide Ikenaka; Masayoshi Sawai; Masatoshi Ishikawa; Hideto Kawaratani; Tsuyoshi Mashitani; Mitsuteru Kitade

    2007-01-01

    Although it is well known that the hepatocellular carcinoma (HCC) is an ominous complication in patients with liver cirrhosis, there has been no approved drug to prevent the development of HCC to date. We previously reported that the combined treatment of vitamin K2 (VK) and angiotensin-converting enzyme inhibitor(ACE-I) significantly suppressed the experimental hepatocarcinogenesis. A 66-year-old Japanese woman with hepatitis C virus (HCV)-related liver cirrhosis developed a dysplastic nodule in the liver detected by enhanced computed tomography along with elevation of the tumor markers, namely, alpha-fetoprotein (AFP)and lectin-reactive demarcation (AFP-L3), suggesting the presence of latent HCC. After oral administration of VK and ACE-I, the serum levels of both AFP and AFP-L3 gradually decreased without any marked alteration of the serum aminotransferase activity. After one-year treatment, not only the serum levels of AFP and AFP-L3 returned to the normal ranges, but also the dysplastic nodule disappeared. Since both VK and ACE-I are widely used without serious side effects, this combined regimen may become a new strategy for chemoprevention against HCC.

  20. MicroRNAs involved in neoplastic transformation of liver cancer stem cells

    Directory of Open Access Journals (Sweden)

    Wang Xinchuan

    2010-12-01

    Full Text Available Abstract Background The existence of cancer stem cells in hepatocellular carcinoma (HCC has been verified by characterizing side population (SP cells based on efflux of Hoechst 33342 dye from stem cells. Recent advances in microRNA (miRNA biology have revealed that miRNAs play an important role in embryonic development and tumorigenesis. However, it is still unclear which miRNAs participate in the neoplastic transformation of liver cancer stem cells (LCSCs during hepatocarcinogenesis. Methods To identify the unique set of miRNAs differentially regulated in LCSCs, we applied SP sorting to primary cultures of F344 rat HCC cancer cells treated with diethylnitrosamine (DEN and normal syngenic fetal liver cells, and the stem-like characteristics of SP cells were verified through detecting expression of CD90.1, AFP and CK-7. Global miRNA expression profiles of two groups of SP cells were screened through microarray platform. Results A total of 68 miRNAs, including miR-10b, miR-21, miR-470*, miR-34c-3p, and let-7i*, were identified as overexpressed in SP of HCC cells compared to fetal liver cells. Ten miRNAs were underexpressed, including miR-200a* and miR-148b*. These miRNAs were validated using stem-loop real-time reverse transcriptase polymerase chain reaction (RT-PCR. Conclusions Our results suggest that LCSCs may have a distinct miRNA expression fingerprint during hepatocarcinogenesis. Dissecting these relationships will provide a new understanding of the function of miRNA in the process of neoplastic transformation of LCSCs.

  1. Global Collaborative STEM Education

    Science.gov (United States)

    Meabh Kelly, Susan; Smith, Walter

    2016-04-01

    Global Collaborative STEM Education, as the name suggests, simultaneously supports two sets of knowledge and skills. The first set is STEM -- science, technology, engineering and math. The other set of content knowledge and skills is that of global collaboration. Successful global partnerships require awareness of one's own culture, the biases embedded within that culture, as well as developing awareness of the collaborators' culture. Workforce skills fostered include open-mindedness, perseverance when faced with obstacles, and resourceful use of technological "bridges" to facilitate and sustain communication. In respect for the 2016 GIFT Workshop focus, Global Collaborative STEM Education projects dedicated to astronomy research will be presented. The projects represent different benchmarks within the Global Collaborative STEM Education continuum, culminating in an astronomy research experience that fully reflects how the global STEM workforce collaborates. To facilitate wider engagement in Global Collaborative STEM Education, project summaries, classroom resources and contact information for established international collaborative astronomy research projects will be disseminated.

  2. Many facets of stem cells

    Institute of Scientific and Technical Information of China (English)

    Jiarui Wu

    2011-01-01

    @@ Research area on stem cells is one of frontiers in biology.The collection of five research articles in this issue aims to cover timely developments in stem cell biology, ranging from generating and identifying stem cell line to manipulating stem cells, and from basic mechanism analysis to applied medical potential.These papers reflect the various research tasks in stem cell biology.

  3. Hepatic stem cells in different histopathologic types of primary hepatic carcinoma%原发性肝癌不同病理组织类型中肝干细胞的分析

    Institute of Scientific and Technical Information of China (English)

    陈川; 王红中; 李增鹏; 王东; 王阁; 索金友; 郑继军; 张志敏; 李琼; 许文; 雒喜中; 邓婧

    2008-01-01

    . Studies on hepatic stem cells are in the early stage at present, and the theory of "stem cell origins" of human primary hepatic carcinoma deserves further verification. OBJECTIVE: To investigate the activation, distribution, origin and immunological expression characteristics of hepatic stem cells in different histopathologic types of primary hepatic carcinoma. DESIGN: Observational comparative study. SETTING: Tumor Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University of Chinese PLA. PARTICIPANTS: Experiments were performed at the Laboratory of Tumor Center, Research Institute of Surgery, Daping Hospital, Third Military Medical University of Chinese PLA from September 2003 to July 2004. We took 94 cases of hepatic cellular cancer, 12 cases of intrahepatic cholangiocellular carcinoma and 10 cases of mixed hepatocarcinoma paraffin-embedded tissue blocks as research objects, with 5 cases of liver cirrhosis and 4 cases of normal liver as experimental control. These materials were collected from the archive of the Department of Pathology of Daping Hospital. Primary hepatic carcinoma tissues and corresponding adjacent liver tissues were obtained from patients who had undergone surgery for the removal of their tumors. All the patients were not treated by chemotherapy or radiotherapy before the operation. They had signed the informed consent. Main Antibodies were bought from Santa Cruz Company.METHODS: The histological and immunohistochemical characteristics were examined by haematoxylin and eosin staining and immunohistochemistry (SP method), including mouse antihuman cytokeratin 19 monoclonal antibody, mouse antihuman cytokeratin 7 monoclonal antibody, mouse antihuman cytokeratin 8&&18 monoclonal antibody, mouse antihuman c-kit monoclonal antibody, mouse antihuman Thy-1 monoclonal antibody, mouse antihuman alpha fetoprotein monoclonal antibody. MAIN OUTCOME MEASURES: Expression of immunological markers of hepatic stem cells in different

  4. STEM CELLS AND PROTEOMICS

    Institute of Scientific and Technical Information of China (English)

    ZHOU Yong-ming; GUO Tian-nan; HUANG Shi-ang

    2006-01-01

    The distinctive features of proteomics are large-scale and high throughput. The key techniques of proteomics are two-dimensional gel electrophoresis, mass spectrometry and bioinformatics. Stem cell can differentiate into all kinds of cells, tissues and organs. There are many proteins and cytokines involved in the process of differentiation. Applying proteomics techniques to the research of the complex process of stem cell differentiation is of great importance to study the mechanism and applications of stem cell differentiation.

  5. Epidermal Stem Cells

    OpenAIRE

    Osman Köse

    2015-01-01

    The epidermis is the outermost layer of the human skin and comprises a multilayered epithelium, the interfollicular epidermis, with associated hair follicles, sebaceous glands, and eccrine sweat glands. There are many origins of stem cells in the skin and skin appendages. These stem cells are localized in different part of the pilosebaseous units and also express many different genes. Epidermal stem cells in the pilosebaseous units not only ensure the maintenance of epidermal homeostasis and ...

  6. Chiaroscuro hematopoietic stem cell.

    OpenAIRE

    Quesenberry, P.; Habibian, M. (PhD); Dooner, M; Zhong, S.; Reilly, J; Peters, S.; De Becker, P; Grimaldi, C.; Carlson, J; REDDY, P; Nilsson, S.; Stewart, F. M.

    1998-01-01

    These observations suggest several immediate clinical strategies. In gene therapy, approaches could be targeted to obtain cycling of hematopoietic stem cells and gene-carrying retrovirus vector integration followed by engraftment at an appropriate time interval which favors engraftment. The same type of approach can be utilized for stem cell expansion approaches. Alternatively marrow or peripheral stem cell engraftment can be obtained with minimal to no toxicity in allochimeric strategies in ...

  7. Cancer stem cell metabolism

    OpenAIRE

    Peiris-Pagès, Maria; Martinez-Outschoorn, Ubaldo E.; Pestell, Richard G.; Sotgia, Federica; Lisanti, Michael P

    2016-01-01

    Cancer is now viewed as a stem cell disease. There is still no consensus on the metabolic characteristics of cancer stem cells, with several studies indicating that they are mainly glycolytic and others pointing instead to mitochondrial metabolism as their principal source of energy. Cancer stem cells also seem to adapt their metabolism to microenvironmental changes by conveniently shifting energy production from one pathway to another, or by acquiring intermediate metabolic phenotypes. Deter...

  8. Lung Stem cell biology

    OpenAIRE

    Ardhanareeswaran, Karthikeyan; Mirotsou, Maria

    2013-01-01

    Over the past few years new insights have been added to the study of stem cells in the adult lung. The exploration of the endogenous lung progenitors as well as the study of exogenously delivered stem cell populations holds promise for advancing our understanding of the biology of lung repair mechanisms. Moreover, it opens new possibilities for the use of stem cell therapy for the development of regenerative medicine approaches for the treatment of lung disease. Here, we discuss the main type...

  9. The leukemic stem cell

    OpenAIRE

    Jordan, Craig T.

    2007-01-01

    Malignant stem cells have recently been described as the source of several types of human cancer. These unique cell types are typically rare and possess properties that are distinct from most other tumor cells. The properties of leukemic stem cells indicate that current chemotherapy drugs will not be effective. The use of current cytotoxic agents is not effective in leukemia because the agents target both the leukemic and normal stem cell populations. Consequently, new strategies are required...

  10. Gastric Cancer Stem Cells

    OpenAIRE

    Takaishi, Shigeo; Okumura, Tomoyuki; Timothy C Wang

    2008-01-01

    Cancer stem cells are defined as the unique subpopulation in the tumors that possess the ability to initiate tumor growth and sustain self-renewal as well as metastatic potential. Accumulating evidence in recent years strongly indicate the existence of cancer stem cells in solid tumors of a wide variety of organs. In this review, we will discuss the possible existence of a gastric cancer stem cell. Our recent data suggest that a subpopulation with a defined marker shows spheroid colony format...

  11. Cancer Stem Cells

    OpenAIRE

    Katarzyna Wieczorek; Jolanta Niewiarowska

    2008-01-01

    Cancer stem cell theory gains increasingly greater significance in the world of medicine. Numerous findings of scientific research in vivo and in vitro indicate that it is the population of undifferentiated, self-renewing cells which is responsible for recurrence of cancer and metastasis. Similarly to normal stem cells, cancer stem cells (CSC) function in the environment of the other cells of the organism, called the niche, where they receive signals for differentiation and proliferation proc...

  12. Prostate cancer stem cells

    OpenAIRE

    Tu, Shi-Ming; Lin, Sue-Hwa

    2011-01-01

    Stem cells have long been implicated in prostate glandular formation. The prostate undergoes regression after androgen deprivation and regeneration after testosterone replacement. Regenerative studies suggest that these cells are found in the proximal ducts and basal layer of the prostate. Many characteristics of prostate cancer indicate that it originates from stem cells. For example, the putative AR− status of prostate stem cells renders them inherently insensitive to androgen blockade ther...

  13. Lung Cancer Stem Cells

    OpenAIRE

    Pine, Sharon R.; Blair Marshall; Lyuba Varticovski

    2008-01-01

    Lung cancer remains a major cause of cancer-related lethality because of high incidence and recurrence in spite of significant advances in staging and therapies. Recent data indicates that stem cells situated throughout the airways may initiate cancer formation. These putative stem cells maintain protumorigenic characteristics including high proliferative capacity, multipotent differentiation, drug resistance and long lifespan relative to other cells. Stem cell signaling and differentiation p...

  14. Optimizing stem cell culture.

    Science.gov (United States)

    van der Sanden, Boudewijn; Dhobb, Mehdi; Berger, François; Wion, Didier

    2010-11-01

    Stem cells always balance between self-renewal and differentiation. Hence, stem cell culture parameters are critical and need to be continuously refined according to progress in our stem cell biology understanding and the latest technological developments. In the past few years, major efforts have been made to define more precisely the medium composition in which stem cells grow or differentiate. This led to the progressive replacement of ill-defined additives such as serum or feeder cell layers by recombinant cytokines or growth factors. Another example is the control of the oxygen pressure. For many years cell cultures have been done under atmospheric oxygen pressure which is much higher than the one experienced by stem cells in vivo. A consequence of cell metabolism is that cell culture conditions are constantly changing. Therefore, the development of high sensitive monitoring processes and control algorithms is required for ensuring cell culture medium homeostasis. Stem cells also sense the physical constraints of their microenvironment. Rigidity, stiffness, and geometry of the culture substrate influence stem cell fate. Hence, nanotopography is probably as important as medium formulation in the optimization of stem cell culture conditions. Recent advances include the development of synthetic bioinformative substrates designed at the micro- and nanoscale level. On going research in many different fields including stem cell biology, nanotechnology, and bioengineering suggest that our current way to culture cells in Petri dish or flasks will soon be outdated as flying across the Atlantic Ocean in the Lindbergh's plane. PMID:20803548

  15. Stem Cell Transplants (For Teens)

    Science.gov (United States)

    ... Can I Help a Friend Who Cuts? Stem Cell Transplants KidsHealth > For Teens > Stem Cell Transplants Print ... it Take to Recover? Coping What Are Stem Cells? As you probably remember from biology class, every ...

  16. The stem factor challenge

    International Nuclear Information System (INIS)

    One of the most important challenges that still needs to be met in the effort to understand the operation of motor-operated, rising-stem valves is the ability to determine stem factor throughout the valve's load range. The stem factor represents the conversion of operator torque to stem thrust. Determining the stem factor is important because some motor-operated valves (MOVs) cannot be tested in the plant at design basis conditions. The ability of these valves to perform their design basis function (typically, to operate against specified flow and pressure loads) must be ensured by analytical methods or by extrapolating from the results of tests conducted at lower loads. Because the stem factor tends to vary in response to friction and lubrication phenomena that occur during loading and wedging, analytical methods and extrapolation methods have been difficult to develop and implement. Early investigations into variability in the stem factor tended to look only at the tip of the iceberg; they focused on what was happening at torque switch trip, which usually occurs at full wedging. In most stems, the stem factor is better (lower) in the wedging transient than before wedging, so working with torque switch trip data alone led many early researchers to false conclusions about the relationship between stem factor and load. However, research at the Idaho National Engineering Laboratory (INEL) has taken a closer look at what happens during the running portion of the closing stroke along with the wedging portion. This shift in focus is important, because functional failure of a valve typically consists of a failure to isolate flow, not a failure to achieve full wedging. Thus, the stem factor that must be determined for a valve's design basis closing requirements is the one that corresponds with the running load before wedging

  17. Aneuploidy in stem cells

    NARCIS (Netherlands)

    Garcia-Martinez, Jorge; Bakker, Bjorn; Schukken, Klaske M; Simon, Judith E; Foijer, Floris

    2016-01-01

    Stem cells hold enormous promise for regenerative medicine as well as for engineering of model systems to study diseases and develop new drugs. The discovery of protocols that allow for generating induced pluripotent stem cells (IPSCs) from somatic cells has brought this promise steps closer to real

  18. Stem cells in urology.

    Science.gov (United States)

    Aboushwareb, Tamer; Atala, Anthony

    2008-11-01

    The shortage of donors for organ transplantation has stimulated research on stem cells as a potential resource for cell-based therapy in all human tissues. Stem cells have been used for regenerative medicine applications in many organ systems, including the genitourinary system. The potential applications for stem cell therapy have, however, been restricted by the ethical issues associated with embryonic stem cell research. Instead, scientists have explored other cell sources, including progenitor and stem cells derived from adult tissues and stem cells derived from the amniotic fluid and placenta. In addition, novel techniques for generating stem cells in the laboratory are being developed. These techniques include somatic cell nuclear transfer, in which the nucleus of an adult somatic cell is placed into an oocyte, and reprogramming of adult cells to induce stem-cell-like behavior. Such techniques are now being used in tissue engineering applications, and some of the most successful experiments have been in the field of urology. Techniques to regenerate bladder tissue have reached the clinic, and exciting progress is being made in other areas, such as regeneration of the kidney and urethra. Cell therapy as a treatment for incontinence and infertility might soon become a reality. Physicians should be optimistic that regenerative medicine and tissue engineering will one day provide mainstream treatment options for urologic disorders.

  19. Making STEM Connections

    Science.gov (United States)

    Stump, Sheryl L.; Bryan, Joel A.; McConnell, Tom J.

    2016-01-01

    Integrated approaches to education in science, technology, engineering, and mathematics (STEM), especially those set in the context of real-world situations, can motivate and deepen students' learning of the STEM subjects (National Academy of Engineering and National Research Council 2014). This article describes two integrated investigations used…

  20. Dazlin' pluripotent stem cells

    NARCIS (Netherlands)

    Welling, M.A.

    2014-01-01

    Pluripotent embryonic stem cells (ESCs) can be isolated from the inner cell mass (ICM) of blastocyst embryos and differentiate into all three germ layers in vitro. However, despite their similar origin, mouse embryonic stem cells represent a more naïve ICM-like pluripotent state whereas human embryo

  1. Stem cells in dermatology.

    Science.gov (United States)

    Ogliari, Karolyn Sassi; Marinowic, Daniel; Brum, Dario Eduardo; Loth, Fabrizio

    2014-01-01

    Preclinical and clinical research have shown that stem cell therapy could be a promising therapeutic option for many diseases in which current medical treatments do not achieve satisfying results or cure. This article describes stem cells sources and their therapeutic applications in dermatology today.

  2. Immunology of Stem Cells and Cancer Stem Cells

    Institute of Scientific and Technical Information of China (English)

    Xiao-Feng Yang

    2007-01-01

    The capacity of pluri-potent stem cells to repair the tissues in which stem cells reside holds great promise in development of novel cell replacement therapeutics for treating chronic and degenerative diseases. However,numerous reports show that stem cell therapy, even in an autologous setting, triggers lymphocyte infiltration and inflammation. Therefore, an important question to be answered is how the host immune system responds to engrafted autologous stem cells or allogeneous stem cells. In this brief review, we summarize the progress in several related areas in this field, including some of our data, in four sections: (1) immunogenicity of stem cells; (2)strategies to inhibit immune rejection to allograft stem cells; (3) immune responses to cancer stem cells; and (4)mesenchymal stem cells in immune regulation. Improvement of our understanding on these and other aspects of immune system-stem cell interplay would greatly facilitate the development of stem cell-based therapeutics for regenerative purposes.

  3. Investigating Preservice STEM Teacher Conceptions of STEM Education

    Science.gov (United States)

    Radloff, Jeff; Guzey, Selcen

    2016-10-01

    Surrounding the national emphasis on improving STEM education, effective STEM educators are required. Connected, yet often overlooked, is the need for effective preservice STEM teaching instruction for incoming educators. At a basic level, preservice STEM teacher education should include STEM content, pedagogy, and conceptualization. However, the literature suggests no leading conception of STEM education, and little is known about how preservice STEM teachers are conceptualizing STEM education. In order to explore preservice STEM teacher conceptions of STEM education, preservice teachers at a large, Midwestern research university were given an open-ended survey eliciting both textual and visual responses. Here, we report and discuss the results of employing this instrument in relation with the current STEM conceptualization literature.

  4. Investigating Preservice STEM Teacher Conceptions of STEM Education

    Science.gov (United States)

    Radloff, Jeff; Guzey, Selcen

    2016-07-01

    Surrounding the national emphasis on improving STEM education, effective STEM educators are required. Connected, yet often overlooked, is the need for effective preservice STEM teaching instruction for incoming educators. At a basic level, preservice STEM teacher education should include STEM content, pedagogy, and conceptualization. However, the literature suggests no leading conception of STEM education, and little is known about how preservice STEM teachers are conceptualizing STEM education. In order to explore preservice STEM teacher conceptions of STEM education, preservice teachers at a large, Midwestern research university were given an open-ended survey eliciting both textual and visual responses. Here, we report and discuss the results of employing this instrument in relation with the current STEM conceptualization literature.

  5. Expanding STEM Education | Poster

    Science.gov (United States)

    Editor’s note: This article is written as a reflection on experiential STEM education by a student who completed her Werner H. Kirsten internship in June 2015. Here, she advocates for incorporating hands-on experience into STEM curricula. If the only way for high school students to learn science, technology, engineering, and mathematics (STEM) is through textbooks, then count me out. But how then do you get students to learn STEM outside of the classroom? The focus of this article is to advocate for high school STEM education through experiential learning. Tom Freston, one of the founders and the chief executive officer (CEO) of MTV Productions, said in an interview in Men’s Journal that “innovation is taking two things that already exist and...

  6. Derivation and characterization of hepatic progenitor cells from human embryonic stem cells.

    Directory of Open Access Journals (Sweden)

    Dongxin Zhao

    Full Text Available The derivation of hepatic progenitor cells from human embryonic stem (hES cells is of value both in the study of early human liver organogenesis and in the creation of an unlimited source of donor cells for hepatocyte transplantation therapy. Here, we report for the first time the generation of hepatic progenitor cells derived from hES cells. Hepatic endoderm cells were generated by activating FGF and BMP pathways and were then purified by fluorescence activated cell sorting using a newly identified surface marker, N-cadherin. After co-culture with STO feeder cells, these purified hepatic endoderm cells yielded hepatic progenitor colonies, which possessed the proliferation potential to be cultured for an extended period of more than 100 days. With extensive expansion, they co-expressed the hepatic marker AFP and the biliary lineage marker KRT7 and maintained bipotential differentiation capacity. They were able to differentiate into hepatocyte-like cells, which expressed ALB and AAT, and into cholangiocyte-like cells, which formed duct-like cyst structures, expressed KRT19 and KRT7, and acquired epithelial polarity. In conclusion, this is the first report of the generation of proliferative and bipotential hepatic progenitor cells from hES cells. These hES cell-derived hepatic progenitor cells could be effectively used as an in vitro model for studying the mechanisms of hepatic stem/progenitor cell origin, self-renewal and differentiation.

  7. 函数法分析AFP和β-hCG在睾丸肿瘤中的变化%Changes of AFP and β-hCG in testicular tumors analyzed by a function method

    Institute of Scientific and Technical Information of China (English)

    关晓峰; 邓耀良; 刘启明; 周德雄; 杨玉恺; 陆鲲; 李飞

    2013-01-01

    目的:建立一种函数法分析AFP和β-hCG在睾丸肿瘤中变化的方法. 方法:首先在睾丸根治术后复查AFP和β-hCG并计算其实测值坐标:以癌标实测值的半衰期个数为横坐标,癌标实测值的对数值为纵坐标.其次计算预测值坐标:设术前癌标实测值为a,半衰期个数为x,经过x个半衰期后预测值的对数值为y(以2为底取对数):y=loga/2x(→)x+y=log2a(公式1),根据公式推导预测值坐标.最后比较癌标实测值与预测值坐标,了解是否有睾丸肿瘤残留或转移. 结果:病例1为卵黄囊瘤和合体滋养层细胞的混合性生殖细胞瘤.其AFP和β-hCG在术后第10天的实测值坐标分别为(2.22,6.21)和(10,8.38),预测值坐标分别为(2.22,6.34)和(10,4.41).该结果提示卵黄囊瘤尚未出现转移,合体滋养层细胞已出现转移.病例2为恶性畸胎瘤和卵黄囊瘤的混合性生殖细胞瘤,其AFP和β-hCG在术后第12天的实测值坐标分别为(2.67,-1.03)和(12,-3.32),预测值坐标分别为(2.67,1.41)和(12,-5.80).但复查AFP和β-hCG的时间分别为2.67和12个半衰期,超过半衰期有效区间,此时癌标实测值均小于其正常值,可认为肿瘤无残留或转移.病例3为胚胎癌,其AFP在术后第1天的实测值坐标为(0.22,9.25),预测值坐标为(0.22,9.55),该结果提示胚胎癌尚未出现转移. 结论:该方法预测的3例患者癌标变化均与实际病程吻合,睾丸根治术后如癌标实测值坐标与预测值坐标吻合,提示肿瘤尚未转移;如不吻合,则提示睾丸肿瘤残留或转移可能.%Objective:To establish a new function method for the analysis of a-fetoprotein (AFP) and β-hCG in testicular tumors. Methods: We reexamined the serum levels of AFP and β-hCG after radical orchiectomy, and calculated the measured coordinate , with the abscissa representing the number of the half-lives of tumor markers, and the ordinate representing the measured value of tumor markers. Referring to the

  8. Mesenchymal stem cells.

    Science.gov (United States)

    Ding, Dah-Ching; Shyu, Woei-Cherng; Lin, Shinn-Zong

    2011-01-01

    Stem cells have two features: the ability to differentiate along different lineages and the ability of self-renewal. Two major types of stem cells have been described, namely, embryonic stem cells and adult stem cells. Embryonic stem cells (ESC) are obtained from the inner cell mass of the blastocyst and are associated with tumorigenesis, and the use of human ESCs involves ethical and legal considerations. The use of adult mesenchymal stem cells is less problematic with regard to these issues. Mesenchymal stem cells (MSCs) are stromal cells that have the ability to self-renew and also exhibit multilineage differentiation. MSCs can be isolated from a variety of tissues, such as umbilical cord, endometrial polyps, menses blood, bone marrow, adipose tissue, etc. This is because the ease of harvest and quantity obtained make these sources most practical for experimental and possible clinical applications. Recently, MSCs have been found in new sources, such as menstrual blood and endometrium. There are likely more sources of MSCs waiting to be discovered, and MSCs may be a good candidate for future experimental or clinical applications. One of the major challenges is to elucidate the mechanisms of differentiation, mobilization, and homing of MSCs, which are highly complex. The multipotent properties of MSCs make them an attractive choice for possible development of clinical applications. Future studies should explore the role of MSCs in differentiation, transplantation, and immune response in various diseases. PMID:21396235

  9. Epidermal Stem Cells

    Directory of Open Access Journals (Sweden)

    Osman Köse

    2015-03-01

    Full Text Available The epidermis is the outermost layer of the human skin and comprises a multilayered epithelium, the interfollicular epidermis, with associated hair follicles, sebaceous glands, and eccrine sweat glands. There are many origins of stem cells in the skin and skin appendages. These stem cells are localized in different part of the pilosebaseous units and also express many different genes. Epidermal stem cells in the pilosebaseous units not only ensure the maintenance of epidermal homeostasis and hair regeneration, but also contribute to repair of the epidermis after injury. In recent years, human induced pluripotent skin stem cells are produced from the epidermal cells such as keratinocytes, fibroblasts and melanocytes. These cells can be transdifferentiated to embriyonic stem cells. Human induced pluripotent stem cells have potential applications in cell replacement therapy and regenerative medicine. These cells provide a means to create valuable tools for basic research and may also produce a source of patient-matched cells for regenerative therapies. In this review, we aimed an overview of epidermal stem cells for better understanding their functions in the skin. Skin will be main organ for using the epidermal cells for regenerative medicine in near future.

  10. Evaluation of squamous cell carcinoma antigen-immunoglobulin M complex (SCCA-IGM) and alpha-L-fucosidase (AFU) as novel diagnostic biomarkers for hepatocellular carcinoma.

    Science.gov (United States)

    Mossad, Nehad A; Mahmoud, Enas H; Osman, Enas A; Mahmoud, Sherif H; Shousha, Hend I

    2014-11-01

    Hepatocellular carcinoma (HCC) surveillance lacks a reliable biomarker. Alpha-fetoprotein (AFP) is the most widely used. However, not all HCCs secrete AFP. AFP may be elevated with cirrhosis in the absence of HCC. Serum alpha-L-fucosidase (AFU) and squamous cell carcinoma antigen-immunoglobulin M complex (SCCA-IgM) were found to be useful markers in diagnosing HCC. SCCA-IgM and AFU were assessed by ELISA technique; AFP was measured by enzyme chemiluminescence in serum of 40 patients with HCC, 30 patients with liver cirrhosis, and 20 healthy control participants to compare their accuracy in early diagnosis of HCC. Serum SCCA-IgM and AFU levels were significantly elevated in HCC group compared to cirrhotic group (P value<0.001 and <0.001, respectively). Receiver operating characteristic curve showed the optimal cutoff value for SCCA-IgM was 233 AU/ml with sensitivity 87.5% and specificity 66% and for AFU was 25 U/L with sensitivity 87.5% and specificity 98%. AFP cutoff value was 48 ng/mL with sensitivity of 70% and specificity of 53.3%. The simultaneous determination of AFP and SCCA-IgM activity increased the sensitivity to 92.5% and specificity to 62.1%. There were positive significant correlations between SCCA-IgM and each of AFU (r=0.296, P=0.005) and AFP (r=0.284, P=0.007) and no correlation between AFP and AFU. All markers did not correlate with the tumor size or affected by the Child score. The significant difference between SCCA-IgM and AFU levels among HCC and cirrhotic patients suggests their use as potential diagnostic tools and allows identifying a new group of HCC patients even in the absence of elevated AFP. PMID:25129443

  11. Fish Stem Cell Cultures

    Directory of Open Access Journals (Sweden)

    Ni Hong, Zhendong Li, Yunhan Hong

    2011-01-01

    Full Text Available Stem cells have the potential for self-renewal and differentiation. First stem cell cultures were derived 30 years ago from early developing mouse embryos. These are pluripotent embryonic stem (ES cells. Efforts towards ES cell derivation have been attempted in other mammalian and non-mammalian species. Work with stem cell culture in fish started 20 years ago. Laboratory fish species, in particular zebrafish and medaka, have been the focus of research towards stem cell cultures. Medaka is the second organism that generated ES cells and the first that gave rise to a spermatogonial stem cell line capable of test-tube sperm production. Most recently, the first haploid stem cells capable of producing whole animals have also been generated from medaka. ES-like cells have been reported also in zebrafish and several marine species. Attempts for germline transmission of ES cell cultures and gene targeting have been reported in zebrafish. Recent years have witnessed the progress in markers and procedures for ES cell characterization. These include the identification of fish homologs/paralogs of mammalian pluripotency genes and parameters for optimal chimera formation. In addition, fish germ cell cultures and transplantation have attracted considerable interest for germline transmission and surrogate production. Haploid ES cell nuclear transfer has proven in medaka the feasibility of semi-cloning as a novel assisted reproductive technology. In this special issue on “Fish Stem Cells and Nuclear Transfer”, we will focus our review on medaka to illustrate the current status and perspective of fish stem cells in research and application. We will also mention semi-cloning as a new development to conventional nuclear transfer.

  12. Fish stem cell cultures.

    Science.gov (United States)

    Hong, Ni; Li, Zhendong; Hong, Yunhan

    2011-04-13

    Stem cells have the potential for self-renewal and differentiation. First stem cell cultures were derived 30 years ago from early developing mouse embryos. These are pluripotent embryonic stem (ES) cells. Efforts towards ES cell derivation have been attempted in other mammalian and non-mammalian species. Work with stem cell culture in fish started 20 years ago. Laboratory fish species, in particular zebrafish and medaka, have been the focus of research towards stem cell cultures. Medaka is the second organism that generated ES cells and the first that gave rise to a spermatogonial stem cell line capable of test-tube sperm production. Most recently, the first haploid stem cells capable of producing whole animals have also been generated from medaka. ES-like cells have been reported also in zebrafish and several marine species. Attempts for germline transmission of ES cell cultures and gene targeting have been reported in zebrafish. Recent years have witnessed the progress in markers and procedures for ES cell characterization. These include the identification of fish homologs/paralogs of mammalian pluripotency genes and parameters for optimal chimera formation. In addition, fish germ cell cultures and transplantation have attracted considerable interest for germline transmission and surrogate production. Haploid ES cell nuclear transfer has proven in medaka the feasibility of semi-cloning as a novel assisted reproductive technology. In this special issue on "Fish Stem Cells and Nuclear Transfer", we will focus our review on medaka to illustrate the current status and perspective of fish stem cells in research and application. We will also mention semi-cloning as a new development to conventional nuclear transfer.

  13. Dental pulp stem cells

    DEFF Research Database (Denmark)

    Ashri, N. Y.; Ajlan, S. A.; Aldahmash, Abdullah M.

    2015-01-01

    Inflammatory periodontal disease is a major cause of loss of tooth-supporting structures. Novel approaches for regeneration of periodontal apparatus is an area of intensive research. Periodontal tissue engineering implies the use of appropriate regenerative cells, delivered through a suitable...... scaffold, and guided through signaling molecules. Dental pulp stem cells have been used in an increasing number of studies in dental tissue engineering. Those cells show mesenchymal (stromal) stem cell-like properties including self-renewal and multilineage differentiation potentials, aside from...... an updated review on dental pulp stem cells and their applications in periodontal regeneration, in combination with different scaffolds and growth factors....

  14. Stem Cells and Cancer

    International Nuclear Information System (INIS)

    Stem cell research has thrived over the last years due to their therapeutic and regenerative potential. Scientific breakthroughs in the field are immediately translated from the scientific journals to the mass media, which is not surprising as the characterisation of the molecular mechanisms that regulate the biology of stem cells is crucial for the treatment of degenerative and cardiovascular diseases, as well as cancer. In the Molecular Oncology Unit at Ciemat we work to unravel the role of cancer stem cells in tumour development, and to find new antitumor therapies. (Author)

  15. Germline stem cells: stems of the next generation

    OpenAIRE

    Yuan, Hebao; Yamashita, Yukiko M

    2010-01-01

    Germline stem cells (GSCs) sustain gametogenesis during the life of organisms. Recent progress has substantially extended our understanding of GSC behavior, including the mechanisms of stem cell self-renewal, asymmetric stem cell division, stem cell niches, dedifferentiation, and tissue aging. GSCs typically are highly proliferative, due to organismal requirement to produce large number of differentiated cells. While many somatic stem cells are multipotent, with potentially multiple different...

  16. Common stemness regulators of embryonic and cancer stem cells

    OpenAIRE

    Hadjimichael, Christiana; Chanoumidou, Konstantina; Papadopoulou, Natalia; Arampatzi, Panagiota; Papamatheakis, Joseph; Kretsovali, Androniki

    2015-01-01

    Pluripotency of embryonic stem cells (ESCs) and induced pluripotent stem cells is regulated by a well characterized gene transcription circuitry. The circuitry is assembled by ESC specific transcription factors, signal transducing molecules and epigenetic regulators. Growing understanding of stem-like cells, albeit of more complex phenotypes, present in tumors (cancer stem cells), provides a common conceptual and research framework for basic and applied stem cell biology. In this review, we h...

  17. Squamous Cell Carcinoma Antigen: A Novel Tumor Marker for Hepatocellular Carcinoma

    International Nuclear Information System (INIS)

    Serum Squamous Cell Carcinoma Antigen (SCC-Ag) by ELISA technique and Alpha-fetoprotein (AFP) by IRMA technique were measured in 65 patients with hepatic focal lesion. 49 patients suffered from proved hepatocellular carcinoma and 16 patients were having cirrhosis and 20 normal controls. Median levels of serum AFP and SCC-Ag in HCC patients was significantly higher when compared with both cirrhotic patients and controls. On using receiver operator characteristic curve to improve sensitivity and specificity of AFP and SCC-Ag for detection of HCC, the best chosen cut-off values were 40 IU/mL for AFP and 2.55 ng/L for SCC-Ag, these yielded a sensitivity of 67.2% and 61.2% respectively and specificity 100%. The diagnostic sensitivity of them increased to 87.7% when they was combiendly calculated. It was found that the combined use of AFP and SCC-Ag is useful in screening patients with hepatic focal lesion to increase the chance of early diagnosis of HCC patients.

  18. The Predictive Power of Serum α-Fetoprotein and Des-γ-Carboxy Prothrombin for Survival Varies by Tumor Size in Hepatocellular Carcinoma.

    Science.gov (United States)

    Tsugawa, Daisuke; Fukumoto, Takumi; Kido, Masahiro; Takebe, Atsushi; Tanaka, Motofumi; Kuramitsu, Kaori; Matsumoto, Ippei; Ajiki, Tetsuo; Koyama, Tatsuki; Ku, Yonson

    2016-01-01

    Alpha-fetoprotein (AFP) and des-γ-carboxy prothrombin (DCP) are frequently used as tumor markers in hepatocellular carcinoma (HCC). The authors hypothesized different patient populations with varying tumor sizes would influence the predictive power of tumor markers for survival in HCC patients. The authors investigated the influence of tumor size on predictive powers of AFP and DCP. 181 patients underwent hepatectomy for HCC from 2003 to 2008 at Kobe University Hospital. Tumor markers were measured before and at 1 month post-hepatectomy. The Cox proportional-hazards model revealed that preoperative serum AFP was associated with survival; its effects depended on tumor size. Hazard ratios (HRs) for preoperative AFP were maximum for medium-sized HCC, and for DCP, HRs were maximum in small-sized tumors. Post-hepatectomy, both tumor markers were associated with survival, revealing significant interactions with tumor size. HRs for postoperative AFP were greater than 1 for relatively wide range tumors (3-11 cm). HRs for postoperative DCP increased with tumor size, with a strong prognostic predictive power for tumors >5 cm. The predictive power of serum tumor markers varied by tumor size in HCC patients. By selecting the appropriate tumor marker, its predictive power can be improved. PMID:27363395

  19. Six cases of malignant germ-cell tumors (grade 4). Comparison with germinoma (grade 2, 3) on CT

    Energy Technology Data Exchange (ETDEWEB)

    Kuramoto, M.; Machida, T.; Maehara, T. (Tokyo Univ. (Japan). Faculty of Medicine)

    1981-08-01

    The CT patterns of six malignant germ-cell tumors other than germinomas (one embryonal carcinoma, one choriocarcinoma, one yolk sac tumor, and three immature teratomas) were compared with those of 12 germinomas (ten suprasellar, one pineal, and one basal ganglia). The effects of irradiation and the changes in the concentration of serum ..cap alpha..-fetoprotein (AFP) and human chorionic gonadotropin (HCG) were compared by means of serial studies. 1. Pre-contrast CT Four of the six (67%) malignant germ-cell tumors showed isodense masses with irregular margins that contained calcified dots and small necrotic cavities. All 12 germinomas showed round, high-density masses with smooth margins without necrotic cavities. The suprasellar germinomas showed no calcification on CT. 2. Post-contrast CT Both malignant germ-cell tumors and germinomas showed a strong enhancement after contrast administration. 3. Change in size after irradiation Malignant germ-cell tumors did not show a decrease in size after radiotherapy (20 - 40 Gy), although the necrotic cavities enlarged in three out of four tumors. Germinomas were highly radiosensitive and showed a remarkable change in size on follow-up CT after irradiation. 4. Serum AFP and HCG The concentrations of serum AFP and/or HCG before therapy were abnormally high in five malignant germ-cell tumors. In three out of four patients, the concentration of serum AFP was normalized, but in one patient the concentration of AFP increased even after irradiation.

  20. Electrochemical immunosensor for simultaneous detection of multiplex cancer biomarkers based on graphene nanocomposites.

    Science.gov (United States)

    Chen, Xia; Jia, Xinle; Han, Jingman; Ma, Jie; Ma, Zhanfang

    2013-12-15

    In this work, a sandwich-format electrochemical immunosensor for simultaneous determination of carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) was fabricated using biofunctional carboxyl graphene nanosheets (CGS) as immunosensing probes, which were fabricated by means of immobilization of toluidine blue (TB) and labeled anti-CEA (Ab2,1), Prussian blue (PB) and anti-AFP (Ab2,2) successively on CGS. The capture anti-CEA (Ab1,1) and anti-AFP (Ab1,2) were immobilized onto the chitosan-Au nanoparticles (CHIT-AuNPs) modified electrode through 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxy succinimide (EDC/NHS). Experimental results revealed that this sandwich-type immunoassay enabled simultaneous detection of CEA and AFP with linear range of 0.5-60 ng mL(-1) for both analytes. The detection limit was 0.1 ng mL(-1) for CEA and 0.05 ng mL(-1) for AFP (S/N=3). The assay results of serum samples with the proposed method were in a good agreement with the reference values from the standard ELISA method. And the negligible cross-reactivity between the two analytes allows it to possess potential promise in clinical diagnosis. PMID:23891798

  1. Genome-wide analysis of DNA methylation in hepatoblastoma tissues

    Science.gov (United States)

    Cui, Ximao; Liu, Baihui; Zheng, Shan; Dong, Kuiran; Dong, Rui

    2016-01-01

    DNA methylation has a crucial role in cancer biology. In the present study, a genome-wide analysis of DNA methylation in hepatoblastoma (HB) tissues was performed to verify differential methylation levels between HB and normal tissues. As alpha-fetoprotein (AFP) has a critical role in HB, AFP methylation levels were also detected using pyrosequencing. Normal and HB liver tissue samples (frozen tissue) were obtained from patients with HB. Genome-wide analysis of DNA methylation in these tissues was performed using an Infinium HumanMethylation450 BeadChip, and the results were confirmed with reverse transcription-quantitative polymerase chain reaction. The Infinium HumanMethylation450 BeadChip demonstrated distinctively less methylation in HB tissues than in non-tumor tissues. In addition, methylation enrichment was observed in positions near the transcription start site of AFP, which exhibited lower methylation levels in HB tissues than in non-tumor liver tissues. Lastly, a significant negative correlation was observed between AFP messenger RNA expression and DNA methylation percentage, using linear Pearson's R correlation coefficients. The present results demonstrate differential methylation levels between HB and normal tissues, and imply that aberrant methylation of AFP in HB could reflect HB development. Expansion of these findings could provide useful insight into HB biology.

  2. Induced pluripotent stem cells

    Institute of Scientific and Technical Information of China (English)

    Siddhartha Bhowmik; LI Yong

    2011-01-01

    Induced pluripotent stem (iPS) cells are a recent development which has brought a promise of great therapeutic values. The previous technique of somatic cell nuclear transfer (SCNT) has been ineffective in humans. Recent discoveries show that human fibroblasts can be reprogrammed by a transient over expression of a small number of genes; they can undergo induced pluripotency. iPS were first produced in 2006. By 2008, work was underway to remove the potential oncogenes from their structure. In 2009, protein iPS (piPS) cells were discovered. Surface markers and reporter genes play an important role in stem cell research. Clinical applications include generation of self renewing stem cells, tissue replacement and many more. Stem cell therapy has the ability to dramatically change the treatment of human diseases.

  3. Innovation and STEM Schools

    Science.gov (United States)

    Roberts, Julia Link

    2015-01-01

    How do schools with a focus on science, technology, engineering, and mathematics (STEM) fit in with state goals to increase innovation and to boost the economy? This article briefly discusses how educators can encourage creativity and innovation.

  4. What are Stem Cells?

    Directory of Open Access Journals (Sweden)

    Ahmadshah Farhat

    2014-05-01

    Full Text Available   Stem cells are undifferentiated self regenerating multi potential cells. There are three types of stem cells categories by the ability to form after cells and correlated with the body’s development process. Totipotent: these stem cells can form an entire organism such as fertilized egg. Ploripotent: ploripotent cells are those that can form any cell in the body but cannot form an entire organism such as developing embryo’s totipotent cells become ploripotent  Multipotent: Multi potent stem cells are those that can only form specific cells in the body such as blood cells based. Based on the sources of stem cells we have three types of these cells: Autologous: Sources of the patient own cells are (Autologous either the cells from patient own body or his or her cord blood. For this type of transplant the physician now usually collects the periphery rather than morrow because the procedure is easier on like a bane morrow harvest it take place outside of an operating room, and the patient does not to be under general unsetting . Allogenic: Sources of stem cells from another donore are primarily relatives (familial allogenic or completely unrelated donors. Xenogenic: In these stem cells from different species are transplanted e .g striatal porcine fetal mesan cephalic (FVM xenotransplants for Parkinson’s disease. On sites of isolation such as embryo, umbilical cord and other body tissues stem cells are named embnyonic, cord blood, and adult stem cells. The scope of results and clinical application of stem cells are such as: Neurodegenerative conditions (MS,ALS, Parkinson’s, Stroke, Ocular disorders- Glaucoma, retinitis Pigmentosa (RP, Auto Immune Conditions (Lupus, MS,R. arthritis, Diabetes, etc, Viral Conditions (Hepatitis C and AIDS, Heart Disease, Adrenal Disorders, Injury(Nerve, Brain, etc, Anti aging (hair, skin, weight control, overall well being/preventive, Emotional disorders, Organ / Tissue Cancers, Blood cancers, Blood diseases

  5. Skeletal (stromal) stem cells

    DEFF Research Database (Denmark)

    Abdallah, Basem M; Kermani, Abbas Jafari; Zaher, Walid;

    2015-01-01

    Skeletal (marrow stromal) stem cells (BMSCs) are a group of multipotent cells that reside in the bone marrow stroma and can differentiate into osteoblasts, chondrocytes and adipocytes. Studying signaling pathways that regulate BMSC differentiation into osteoblastic cells is a strategy....../preadipocyte factor 1 (Dlk1/Pref-1), the Wnt co-receptor Lrp5 and intracellular kinases. This article is part of a Special Issue entitled: Stem Cells and Bone....

  6. Stem cell myths

    OpenAIRE

    Magnus, Tim; Liu, Ying; Parker, Graham C.; Rao, Mahendra S.

    2007-01-01

    Stem cells, although difficult to define, hold great promise as tools for understanding development and as therapeutic agents. However, as with any new field, uncritical enthusiasm can outstrip reality. In this review, we have listed nine common myths that we believe affect our approach to evaluating stem cells for therapy. We suggest that careful consideration needs to be given to each of these issues when evaluating a particular cell for its use in therapy. Data need to be collected and rep...

  7. Stem cell mechanobiology

    OpenAIRE

    David A. Lee; Knight, Martin M.; Jonathan J Campbell; Bader, Dan L.

    2010-01-01

    Stem cells are undifferentiated cells that are capable of proliferation, self-maintenance and differentiation towards specific cell phenotypes. These processes are controlled by a variety of cues including physicochemical factors associated with the specific mechanical environment in which the cells reside. The control of stem cell biology through mechanical factors remains poorly understood and is the focus of the developing field of mechanobiology. This review provides an insight into the c...

  8. Aneuploidy in stem cells

    OpenAIRE

    Garcia-Martinez, Jorge; Bakker, Bjorn; Schukken, Klaske M; Simon, Judith E; Foijer, Floris

    2016-01-01

    Stem cells hold enormous promise for regenerative medicine as well as for engineering of model systems to study diseases and develop new drugs. The discovery of protocols that allow for generating induced pluripotent stem cells (IPSCs) from somatic cells has brought this promise steps closer to reality. However, as somatic cells might have accumulated various chromosomal abnormalities, including aneuploidies throughout their lives, the resulting IPSCs might no longer carry the perfect bluepri...

  9. Exploring the STEM shortage

    Science.gov (United States)

    Gates, S. James, Jr.; Bhulai, Alfred; IcePilot; JoeB

    2014-12-01

    In reply to Margaret Harris' article on "The STEM shortage paradox" (Graduate careers, October pp56-59, http://ow.ly/DXvIq) and a related post on the physicsworld.com blog ("The STEM employment paradox, revisited", http://ow.ly/DXvCl), both of which questioned reports that the UK is suffering from a severe shortage of graduates in science, technology, engineering and mathematics.

  10. Stem cell therapy independent of stemness

    OpenAIRE

    Lee, Techung

    2012-01-01

    Mesenchymal stem cell (MSC) therapy is entering a new era shifting the focus from initial feasibility study to optimization of therapeutic efficacy. However, how MSC therapy facilitates tissue regeneration remains incompletely characterized. Consistent with the emerging notion that secretion of multiple growth factors/cytokines (trophic factors) by MSC provides the underlying tissue regenerative mechanism, the recent study by Bai et al demonstrated a critical therapeutic role of MSC-derived h...

  11. 3种血清肿瘤标志物检测在原发性肝癌诊断中价值%Value of three serum tumor markers to the diagnosis of primary hepatocellular carcinoma

    Institute of Scientific and Technical Information of China (English)

    王莉; 贾志凌; 刘畅; 柴丽娜

    2013-01-01

    Objective To investigate the diagnostic value of alpha-fetoprotein (AFP),lectin-reactive alpha-fetoprotein (AFP L3) and Golgi protein 73 (GP73) to the diagnosis of primary hepatocellular carcinoma.Methods Serum AFP was detected by using electrochemiluminescence assay in 102 patients with primary hepatocellular carcinoma,79 patients with benign liver diseases and 31 healthy volunteers.AFP-L3 was detected by using affinitive centrifugal tube in patients with positive AFP (>20 μg/L).GP73 was quantitatively analyzed by using ELISA.Results The sensitivities of AFP,AFP-L3 and GP73 were 51.96%,75.50% and 77.45%,and the specificities were 50.63%,93.67% and 71.95% respectively in the diagnosis of primary hepatocellular carcinoma.The sensitivity and specificity of the combined detection of three markers were 95.10% and 93.67% in the diagnosis of hepatocellular carcinoma.The positive rate of AFP-L3 was 96.23% in patients with AFP>400 ng/mL,obviously higher than that in those with AFP≤400 ng/mL (53.06%) (P<0.05),and there was no significant difference in the positive rate of GP73 between them (75.47% vs 75.51%) (P>0.05).Conclusion The combined detection of AFP,AFP-L3 and GP73 can improve the sensitivity and specificity in the diagnosis of primary hepatocellular carcinoma.GP73 is superior to AFP in the early diagnosis of primary hepatocellular carcinoma.%目的 探讨血清甲胎蛋白(alpha-fetoprotein,AFP)、甲胎蛋白异质体(lectin-reactive alpha-fetoprotein,AFP-L3)及高尔基体糖蛋白73(Golgi protein 73,GP73)检测诊断原发性肝癌的价值.方法 102例原发性肝癌患者(肝癌组),79例良性肝病患者(良性肝病组)和体检健康者31例(对照组),采用电化学发光法检测3组AFP,AFP阳性者(>20μg/L)采用亲和吸附离心管法检测AFP-L3,采用酶联免疫法检测GP73.结果 AFP、AFP-L3和GP73对原发性肝癌诊断的灵敏度分别为51.96%、75.50%、77.45%,特异性分别为50.63%、93

  12. Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

    Directory of Open Access Journals (Sweden)

    Zhong G

    2015-03-01

    Full Text Available Guangxian Zhong,1,2,* Ruilong Lan,3,* Wenxin Zhang,1,4 Feihuan Fu,5 Yiming Sun,1,4 Huaping Peng,1,4 Tianbin Chen,3 Yishan Cai,6 Ailin Liu,1,4 Jianhua Lin,2 Xinhua Lin1,4 1Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University, 2Department of Orthopaedics, 3The Centralab, First Affiliated Hospital of Fujian Medical University, 4Nano Medical Technology Research Institute, Fujian Medical University, Fuzhou, 5Department of Endocrinology, The County Hospital of Anxi, Anxi, 6Fujian International Travel Healthcare Center, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou, People’s Republic of China *These authors contributed equally to this work Abstract: A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5. The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis. Keywords: electrochemical immunosensors, three-dimensional nanostructure gold electrode, square-wave oxidation-reduction cycle, alpha-fetoprotein 

  13. 应用ROC曲线评价AFP、AFU、5'-NT在原发性肝癌诊断中的意义%Assessment on the Value of AFP, AFU and 5' -NT in Diagnosis of Primary Hepatic Carcinoma by ROC Curve

    Institute of Scientific and Technical Information of China (English)

    陈建军; 陈建国; 高晓阳; 吴白平

    2012-01-01

    目的 探讨α-L-岩藻糖苷酶(AFU)、甲胎蛋白(AFP)、5’-核苷酸酶(5’- NT)检测对原发性肝癌(PHC)患者的诊断价值. 方法 测定125例肝癌患者及50例体检者作为对照组,应用ROC曲线进行比较与分析. 结果 AFU、AFP和5’- NT检测指标在两组资料间比较差异均有统计学意义.AFP、AFU和5’- NT三种指标单独检测时,ROC曲线下面积分别为0.707、0.803、0.817.三项标志物单项检测PHC的敏感性分别为0.68、0.81、0.80,联合检测AFP和AFU或5'-NT可使PHC检出率提高至0.888和0.872,三项标志物联合检测PHC的检出率高达0.92. 结论 通过ROC曲线评价,联合检测AFP、AFU和5’- NT三种指标可以提高对原发性肝癌(PHC)患者的诊断价值.%Objective To explore the diagnostic value of α - fetoprotein (AFP), AFU (α - L - fucosidase), 5' - NT (5' — nucleotidase) and their combinations for primary hepatic carcinoma (PHC). Methods Serum AFP, AFU and 5' - NT were detected in 125 patients with PHC (PHC group) and 50 healthy people (control group). The test results were compared and analyzed by ROC curve. Results The levels of AFP, AFU and 5' - NT were significantly different between the two groups (P < 0.05). The areas under ROC curves of AFP, AFU and 5' - NT of PHC patients were 0.707, 0.803 and 0.817 respectively. The sensitivity of AFP, AFU and 5' - NT for PHC diagnosis was 0.68,0.81 and 0. 80 respectively. Combination of AFP and AFU or 5' - NT could increase the sensitivity to 0.888 and 0.872, and combined detection of AFP, AFU and 5' - NT could raise the sensitivity to 0.92. Conclusions Roc curve suggests that the combined detection of AFP, AFU and 5' - NT is superior to any single index in PHC diagnosis. It can greatly increase the detection of PHC.

  14. Expression of biomarker genes of differentiation in D3 mouse embryonic stem cells after exposure to different embryotoxicant and non-embryotoxicant model chemicals

    Directory of Open Access Journals (Sweden)

    Andrea C. Romero

    2015-12-01

    Full Text Available There is a necessity to develop in vitro methods for testing embryotoxicity (Romero et al., 2015 [1]. We studied the progress of D3 mouse embryonic stem cells differentiation exposed to model embryotoxicants and non-embryotoxicants chemicals through the expression of biomarker genes. We studied a set of 16 different genes biomarkers of general cellular processes (Cdk1, Myc, Jun, Mixl, Cer and Wnt3, ectoderm formation (Nrcam, Nes, Shh and Pnpla6, mesoderm formation (Mesp1, Vegfa, Myo1e and Hdac7 and endoderm formation (Flk1 and Afp. We offer dose response in order to derive the concentration causing either 50% or 200% of expression of the biomarker gene. These records revealed to be a valuable end-point to predict in vitro the embryotoxicity of chemicals (Romero et al., 2015 [1].

  15. ROM Plus®: accurate point-of-care detection of ruptured fetal membranes

    Directory of Open Access Journals (Sweden)

    McQuivey RW

    2016-05-01

    Full Text Available Ross W McQuivey,1 Jon E Block2 1Clinical Affairs, Clinical Innovations, Salt Lake City, UT, 2Independent Clinical Consultant, San Francisco, CA, USAAbstract: Accurate and timely diagnosis of rupture of fetal membranes is imperative to inform and guide gestational age-specific interventions to optimize perinatal outcomes and reduce the risk of serious complications, including preterm delivery and infections. The ROM Plus is a rapid, point-of-care, qualitative immunochromatographic diagnostic test that uses a unique monoclonal/polyclonal antibody approach to detect two different proteins found in amniotic fluid at high concentrations: alpha-fetoprotein and insulin-like growth factor binding protein-1. Clinical study results have uniformly demonstrated high diagnostic accuracy and performance characteristics with this point-of-care test that exceeds conventional clinical testing with external laboratory evaluation. The description, indications for use, procedural steps, and laboratory and clinical characterization of this assay are presented in this article.Keywords: ROM Plus®, premature rupture of membranes, point-of-care immunoassay, insulin-like growth factor binding protein-1, IGFBP-1, placental protein 12, PP12, alpha-fetoprotein, AFP

  16. Fucosylation Is a Promising Target for Cancer Diagnosis and Therapy

    Directory of Open Access Journals (Sweden)

    Shinichiro Shinzaki

    2012-01-01

    Full Text Available Oligosaccharides, sequences of carbohydrates conjugated to proteins and lipids, are arguably the most abundant and structurally diverse class of molecules. Fucosylation is one of the most important oligosaccharide modifications involved in cancer and inflammation. Recent advances in glycomics have identified several types of glyco-biomarkers containing fucosylation that are linked to certain types of cancer. Fucosylated alpha-fetoprotein (AFP is widely used in the diagnosis of hepatocellular carcinoma because it is more specific than alpha-fetoprotein. High levels of fucosylated haptoglobin have also been found in sera of patients with various carcinomas. We have recently established a simple lectin-antibody ELISA to measure fucosylated haptoglobin and to investigate its clinical use. Cellular fucosylation is dependent upon fucosyltransferase activity and the level of its donor substrate, guanosine diphosphate (GDP-fucose. GDP-mannose-4,6-dehydratase (GMDS is a key enzyme involved in the synthesis of GDP-fucose. Mutations of GMDS found in colon cancer cells induced a malignant phenotype, leading to rapid growth in athymic mice resistant to natural killer cells. This review describes the role of fucosylated haptoglobin as a cancer biomarker, and discusses the possible biological role of fucosylation in cancer development.

  17. Limbal stem cell transplantation

    Directory of Open Access Journals (Sweden)

    Fernandes Merle

    2004-01-01

    Full Text Available The past two decades have witnessed remarkable progress in limbal stem cell transplantation. In addition to harvesting stem cells from a cadaver or a live related donor, it is now possible to cultivate limbal stem cells in vitro and then transplant them onto the recipient bed. A clear understanding of the basic disease pathology and a correct assessment of the extent of stem cell deficiency are essential. A holistic approach towards management of limbal stem cell deficiency is needed. This also includes management of the underlying systemic disease, ocular adnexal pathology and dry eye. Conjunctival limbal autografts from the healthy contralateral eye are performed for unilateral cases. In bilateral cases, tissue may be harvested from a cadaver or a living related donor; prolonged immunosuppression is needed to avoid allograft rejection in such cases. This review describes the surgical techniques, postoperative treatment regimes (including immunosuppression for allografts, the complications and their management. The short and long-term outcomes of the various modalities reported in the literature are also described.

  18. Epidemiological Analysis and Monitoring System Evaluation of AFP Cases in Shijiazhuang City during 2005-2010%2005-2010年石家庄市急性弛缓性麻痹病例流行病学分析与监测系统评价

    Institute of Scientific and Technical Information of China (English)

    喻文雅; 李怡秋; 史春伟; 刘君卿

    2011-01-01

    [Objective]To analyze the epidemiological characteristics of acute flaccid paralysis( AFP) and its monitoring results so as to guide the maintenance of the poliomyelitis free status in Shijiazhuang City. [ Methods]All the monitoring data of AFP reported in Shijiazhuang from 2005 to 2010 were collected and analyzed by descriptive epidemiology. [ Results]A total of 280 local AFP cases of children under 15 years old were reported in Shijiazhuang from 2005 to 2010 with annual average incidence of 2.60/ 100 000. No wild strain of poliovirus or poliomyelitis case were found. The monitoring systems sensitivity, timeliness and integrity of AFP surveillance system exceed the relevant requirements of Ministry of Health. [ Conclusion]The Surveillance System of AFP in Shijiazhuang have met the requirements of WHO and Ministry of Health of China. The status of poliomyelitis free has been maintained.%目的 分析石家庄市急性弛缓性麻痹(AFP)病例流行病学特征及监测结果,指导维持本市无脊髓灰质炎(脊灰)工作.方法 收集2005-2010年全市报告的AFP病例个案调查表及其相关报表资料,采用描述流行病学方法进行分析.结果 石家庄市2005-2010年共报告15岁以下本地儿童AFP病例280例,年平均报告发病率2.60/10万,无脊灰病毒野毒株检出和脊髓灰质炎病例报告;系统监测的敏感性、及时性和完整性指标均明显超过卫生部要求.结论 石家庄市AFP病例监测系统各项指标均达到世界卫生组织和卫生部的要求,保持了无脊灰状态.

  19. 血清肿瘤标志物AFP、CEA和CA199联合检测在消化系统恶性肿瘤诊断中的临床价值%The clinical value of combined detection of serum tumor markers AFP, CEA and CA199 in the diagnosis of digestive system malignancies

    Institute of Scientific and Technical Information of China (English)

    徐惠明; 周明霞; 张群雄; 沈伟春

    2011-01-01

    目的 探讨血清肿瘤标志物甲胎蛋白(AFP)、癌胚抗原(CEA)、糖类抗原199(CA199)联合检测在消化系统恶性肿瘤诊断中的临床价值.方法 回顾性分析203例因消化系统疾病住院的患者,经内镜、病理及腹部CT确诊,其中消化系统恶性肿瘤125例,消化系统良性疾病78例,均应用化学发光法检测血清AFP、CEA和CA199的水平.结果 恶性肿瘤患者AFP、CEA、CA199均高于良性消化疾病患者,差异有统计学意义(P<0.05或P<0.01).AFP、CEA与CA199联合检测较单项检测对消化系统恶性肿瘤诊断的敏感度更高.结论 血清肿瘤标志物AFP、CEA和CA199检测作为诊断性试验,在消化道恶性肿瘤的诊断中具有重要的价值,而联合检测使敏感度得到明显提高,降低了消化道肿瘤的漏诊率.%Objective To evaluate and discuss the clinical value of combined detection of serum tumor makers AFP, CEA and CA199 in several diagnosed malignancies of digestive system.Methods The single and combined detecting results of AFP, CEA, CA199 in 203 patients with digestive tract diseases were retrospectively analyzed. Seventy - eight patients with benign diseases and 125 patients with malignant tumors were diagnosed by endoscopy, pathology and abdominal CT. Results The patients with malignant tumors had higher levels of AFP, CEA and CA199 that of the patients with benign diseases(P<0.05 or P<0.01). The combined detection of AFP, CEA and CA199 made the sensitivity increased. Conclusion Serum tumor markers AFP, CEA and CA199 are of an important value as a diagnostic test for malignant tumors in digestive system. The combined detection can improve the sensitivity and reduce the rate of missed diagnosis of gastrointestinal cancer.

  20. Advancing Stem Cell Biology toward Stem Cell Therapeutics

    OpenAIRE

    Scadden, David; Srivastava, Alok

    2012-01-01

    Here, the International Society for Stem Cell Research (ISSCR) Clinical Translation Committee introduces a series of articles outlining the current status, opportunities, and challenges surrounding the clinical translation of stem cell therapeutics for specific medical conditions.

  1. Simultaneous quantitative detection of multiple tumor markers with a rapid and sensitive multicolor quantum dots based immunochromatographic test strip.

    Science.gov (United States)

    Wang, Chunying; Hou, Fei; Ma, Yicai

    2015-06-15

    A novel multicolor quantum dots (QDs) based immunochromatographic test strip (ICTS) was developed for simultaneous quantitative detection of multiple tumor markers, by utilizing alpha fetoprotein (AFP) and carcinoembryonic antigen (CEA) as models. The immunosensor could realize simultaneous quantitative detection of tumor markers with only one test line and one control line on the nitrocellulose membrane (NC membrane) due to the introduction of multicolor QDs. In this method, a mixture of mouse anti-AFP McAb and mouse anti-CEA McAb was coated on NC membrane as test line and goat anti-mouse IgG antibody was coated as control line. Anti-AFP McAb-QDs546 conjugates and anti-CEA McAb-QDs620 conjugates were mixed and applied to the conjugate pad. Simultaneous quantitative detection of multiple tumor markers was achieved by detecting the fluorescence intensity of captured QDs labels on test line and control line using a test strip reader. Under the optimum conditions, AFP and CEA could be detected as low as 3 ng/mL and 2 ng/mL in 15 min with a sample volume of 80 μL, and no obvious cross-reactivity was observed. The immunosensor was validated with 130 clinical samples and in which it exhibited high sensitivity (93% for AFP and 87% for CEA) and specificity (94% for AFP and 97% for CEA). The immunosensor also demonstrated high recoveries (87.5-113% for AFP and 90-97.3% for CEA) and low relative standard deviations (RSDs) (2.8-6.2% for AFP and 4.9-9.6% for CEA) when testing spiked human serum. This novel multicolor QDs based ICTS provides an easy and rapid, simultaneous quantitative detecting strategy for point-of-care testing of tumor markers. PMID:25562743

  2. Stem Properties of Autobacteria

    Directory of Open Access Journals (Sweden)

    Boris A. Berdichevskyy

    2014-03-01

    Full Text Available This paper discusses the stem properties of the autobacteria as part of the activation syndrome and persistence of the endogenous microflora in the adaptation process of the macroorganism to stress, as well as the ability of the bacteria to stimulate the cells of the macroorganism to manifest stem properties. The essence of this syndrome involves testing the tissue with the autobacteria of their "host" body to identify the foci of cellular insolvency with the subsequent inclusion of the autostrains in the implementation phase of the catabolic and anabolic inflammations. Considering the genetic tropism of the microbes to the organ-specific tissue of the area affected, the existence of the stem properties of the autobacteria is assumed to be realized in the process of reparative regeneration. The great clinical significance of further study of this phenomenon is not excluded.

  3. The Stem Cell Conundrum

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    @@ At the beginning of this year, Kelly Reynolds,a US-national diagnosed with amyotrophic lateral sclerosis (ALS), became the one of the latest overseas patient to undergo stem cell treatment at the Nanshan Hospital in Shenzhen.Confined to a wheelchair and with limited use of his hands,the 39-year old received four fetal stem cell injections over a three-week period. So far,the results have been positive and Reynolds, acording to his personal blog page, is upbeat about the long-term benefits.

  4. Limbal Stem Cell Therapy

    OpenAIRE

    Kringlegarden, Hilde Grane

    2013-01-01

    It is widely accepted today that stem cells in the adult corneal epithelium is located to the limbus. No specific marker of limbal epithelial cells (LESCs) has been identified, yet many have been suggested, including ΔNp63α, ABCG2, vimentin and notch 1. Negative markers include amongst others the differentiation markers Ck3 and Ck12. The lack of an identified specific marker elucidates the need for establishment of more exact molecular markers of LESCs. Limbal stem cell deficiency (LSCD) may ...

  5. Stem cell therapy for diabetes

    Directory of Open Access Journals (Sweden)

    K O Lee

    2012-01-01

    Full Text Available Stem cell therapy holds immense promise for the treatment of patients with diabetes mellitus. Research on the ability of human embryonic stem cells to differentiate into islet cells has defined the developmental stages and transcription factors involved in this process. However, the clinical applications of human embryonic stem cells are limited by ethical concerns, as well as the potential for teratoma formation. As a consequence, alternative forms of stem cell therapies, such as induced pluripotent stem cells, umbilical cord stem cells and bone marrow-derived mesenchymal stem cells, have become an area of intense study. Recent advances in stem cell therapy may turn this into a realistic treatment for diabetes in the near future.

  6. Bone marrow (stem cell) donation

    Science.gov (United States)

    ... this page: //medlineplus.gov/ency/patientinstructions/000839.htm Bone marrow (stem cell) donation To use the sharing ... stem cells from a donor's blood. Types of Bone Marrow Donation There are two types of bone ...

  7. Engineering stem cell niches in bioreactors

    OpenAIRE

    2013-01-01

    Stem cells, including embryonic stem cells, induced pluripotent stem cells, mesenchymal stem cells and amniotic fluid stem cells have the potential to be expanded and differentiated into various cell types in the body. Efficient differentiation of stem cells with the desired tissue-specific function is critical for stem cell-based cell therapy, tissue engineering, drug discovery and disease modeling. Bioreactors provide a great platform to regulate the stem cell microenvironment, known as “ni...

  8. Stem cell therapy independent of stemness.

    Science.gov (United States)

    Lee, Techung

    2012-12-26

    Mesenchymal stem cell (MSC) therapy is entering a new era shifting the focus from initial feasibility study to optimization of therapeutic efficacy. However, how MSC therapy facilitates tissue regeneration remains incompletely characterized. Consistent with the emerging notion that secretion of multiple growth factors/cytokines (trophic factors) by MSC provides the underlying tissue regenerative mechanism, the recent study by Bai et al demonstrated a critical therapeutic role of MSC-derived hepatocyte growth factor (HGF) in two animal models of multiple sclerosis (MS), which is a progressive autoimmune disorder caused by damage to the myelin sheath and loss of oligodendrocytes. Although current MS therapies are directed toward attenuation of the immune response, robust repair of myelin sheath likely requires a regenerative approach focusing on long-term replacement of the lost oligodendrocytes. This approach appears feasible because adult organs contain various populations of multipotent resident stem/progenitor cells that may be activated by MSC trophic factors as demonstrated by Bai et al This commentary highlights and discusses the major findings of their studies, emphasizing the anti-inflammatory function and trophic cross-talk mechanisms mediated by HGF and other MSC-derived trophic factors in sustaining the treatment benefits. Identification of multiple functionally synergistic trophic factors, such as HGF and vascular endothelial growth factor, can eventually lead to the development of efficacious cell-free therapeutic regimens targeting a broad spectrum of degenerative conditions. PMID:23516128

  9. Stem cell therapy for diabetes

    OpenAIRE

    Lee, K. O.; Gan, S U; Calne, R Y

    2012-01-01

    Stem cell therapy holds immense promise for the treatment of patients with diabetes mellitus. Research on the ability of human embryonic stem cells to differentiate into islet cells has defined the developmental stages and transcription factors involved in this process. However, the clinical applications of human embryonic stem cells are limited by ethical concerns, as well as the potential for teratoma formation. As a consequence, alternative forms of stem cell therapies, such as induced plu...

  10. Introduction to Stem Cell Therapy

    OpenAIRE

    Biehl, Jesse K.; Russell, Brenda

    2009-01-01

    Stem cells have the ability to differentiate into specific cell types. The two defining characteristics of a stem cell are perpetual self-renewal and the ability to differentiate into a specialized adult cell type. There are two major classes of stem cells: pluripotent that can become any cell in the adult body, and multipotent that are restricted to becoming a more limited population of cells. Cell sources, characteristics, differentiation and therapeutic applications are discussed. Stem cel...

  11. The new stem cell biology.

    OpenAIRE

    Quesenberry, Peter J.; Colvin, Gerald A; Lambert, Jean-Francois; Frimberger, Angela E.; Dooner, Mark S.; Mcauliffe, Christina I.; Miller, Caroline; Becker, Pamela; Badiavas, Evangelis; Falanga, Vincent J.; Elfenbein, Gerald; Lum, Lawrence G.

    2002-01-01

    Recent studies have indicated that bone marrow stem cells are capable of generating muscle, cardiac, hepatic, renal, and bone cells. Purified hematopoietic stem cells have generated cardiac and hepatic cells and reversed disease manifestations in these tissues. Hematopoietic stem cells also alter phenotype with cell cycle transit or circadian phase. During a cytokine stimulated cell cycle transit, reversible alterations of differentiation and engraftment occur. Primitive hematopoietic stem ce...

  12. Stem cell research in China

    OpenAIRE

    Liao, Lianming; Li, Lingsong; Zhao, Robert Chunhua

    2007-01-01

    In the past 5 years, China has increased its efforts in the field of stem cell research and practice. Basic research mainly focuses on bone marrow and embryonic stem cells. Clinical applications of stem cells in the treatment of acute heart failure, acute liver failure and lower limb ischaemia have been reported by many hospitals. China enacted its ‘Ethical Guidelines for Human Embryonic Stem Cell Research’ in 2003. At present, China has the most liberal and favourable environments for human ...

  13. Stem cell organization in Arabidopsis

    OpenAIRE

    Wendrich, J.R.

    2016-01-01

    Growth of plant tissues and organs depends on continuous production of new cells, by niches of stem cells. Stem cells typically divide to give rise to one differentiating daughter and one non-differentiating daughter. This constant process of self-renewal ensures that the niches of stem cells or meristems stay active throughout plant-life. Specification of stem cells occurs very early during development of the emrbyo and they are maintained during later stages. The Arabidopsis embryo is a hig...

  14. Laser biomodulation on stem cells

    Science.gov (United States)

    Liu, Timon C.; Duan, Rui; Li, Yan; Li, Xue-Feng; Tan, Li-Ling; Liu, Songhao

    2001-08-01

    Stem cells are views from the perspectives of their function, evolution, development, and cause. Counterintuitively, most stem cells may arise late in development, to act principally in tissue renewal, thus ensuring an organisms long-term survival. Surprisingly, recent reports suggest that tissue-specific adult stem cells have the potential to contribute to replenishment of multiple adult tissues. Stem cells are currently in the news for two reasons: the successful cultivation of human embryonic stem cell lines and reports that adult stem cells can differentiate into developmentally unrelated cell types, such as nerve cells into blood cells. The spotlight on stem cells has revealed gaps in our knowledge that must be filled if we are to take advantage of their full potential for treating devastating degenerative diseases such as Parkinsons's disease and muscular dystrophy. We need to know more about the intrinsic controls that keep stem cells as stem cells or direct them along particular differentiation pathways. Such intrinsic regulators are, in turn, sensitive to the influences of the microenvironment, or niche, where stem cells normally reside. Both intrinsic and extrinsic signals regular stem cell fate and some of these signals have now been identified. Vacek et al and Wang et al have studied the effect of low intensity laser on the haemopoietic stem cells in vitro. There experiments show there is indeed the effect of low intensity laser on the haemopoietic stem cells in vitro, and the present effect is the promotion of haemopoietic stem cells proliferation. In other words, low intensity laser irradiation can act as an extrinsic signal regulating stem cell fate. In this paper, we study how low intensity laser can be used to regulate stem cell fate from the viewpoint of collective phototransduction.

  15. Stem cells and genetic diseases

    Directory of Open Access Journals (Sweden)

    Irshad S.

    2012-09-01

    Full Text Available In this review, we have discussed a role of stem cells in the treatment of genetic diseases including cochlear and retinal regeneration. The most perceptive use of stem cells at the genetic diseases is cellular repair of tissues affected by a genetic mutation when stem cells without such mutation are transplanted to restore normal tissue function.

  16. Stem cell organization in Arabidopsis

    NARCIS (Netherlands)

    Wendrich, J.R.

    2016-01-01

    Growth of plant tissues and organs depends on continuous production of new cells, by niches of stem cells. Stem cells typically divide to give rise to one differentiating daughter and one non-differentiating daughter. This constant process of self-renewal ensures that the niches of stem cells or mer

  17. Stem Cell Transplants (For Parents)

    Science.gov (United States)

    ... Story" 5 Things to Know About Zika & Pregnancy Stem Cell Transplants KidsHealth > For Parents > Stem Cell Transplants Print A A A Text Size What's ... Recovery Coping en español Trasplantes de células madre Stem cells are cells in the body that have the ...

  18. 布加综合征肿瘤相关血清学检查的临床意义%Clinical significances of AFP,CA199 and CA125 levels in Budd Chiari syndrome

    Institute of Scientific and Technical Information of China (English)

    许伟; 徐浩; 祖茂衡; 顾玉明; 邹文卫; 张庆桥; 魏宁

    2013-01-01

    Objective To explore the clinical significances of serum AFP,CA199 and CA125 levels in patients with Budd-Chiari syndrome(BCS). Methods A retrospective analysis of serum AFP,CA125 ,CA1 99 levels was carried out in 188 patients with BCS,in order to analyze the differences between subtypes of the disease. The scrum CA125,CA199 levels of 188 patients were compared with hepatic cirrhosis and healthy people,so as to evaluate the value of CA125,CA199 in the diagnosis of BCS. Results For the levels of serum CA125 and CA199,there was significant difference between patients with hepatic cirrhosis , BCS and healthy control group (P<0. 05) ; for the level of serum CA199, there was significant different between patients with hepatic cirrhosis and BCS( P<0. 01); there was no significant difference between patients with hepatic cirrhosis and BCS for the level of serum CA125. There was no significant difference between subtypes of BCS for the levels of serum CA125 ,CA1 99. CA199 was useful in judging hepatic cirrhosis and BCS, cutoff point was 31. 14 U/mL,and the sensitivity,specificity were 64 % ,85. o% , respectively. The standard of AFP in BCS was (3. 96 ± 2. 45)μg/L( 95%CI 0 - 8. 76μg/L) , between the overall standard (0 - 20 μg/L) of normal population. The standards of 16 BCS patients with hcpatocarcinoma:4 cases were greater than 1000μg/L,6 cases were in 20 - 400μg/L,7 cases were less than 20μg/ L. Conclusion The mean AFP standard of BCS is in the normal range. The amount of CA125,CA199 is greatly related to evaluate the degree of liver injury and judged the progress of BCS.%目的 探讨布加综合征(BCS)肿瘤相关血清学检查如AFP、CA125、CA199变化的临床意义.方法 回顾分析188例BCS肿瘤相关血清AFP、CA125、CA199水平,分析BCS不同亚型之间的差异,并与肝硬化血清、正常体检人群AFP、CA125、CA199水平对照.ROC分析评价CA199、CA125对BCS、肝硬化的诊断价值.结果 BCS组、肝硬化组血清CA125、CA199

  19. Level of serum AFP in patients with Budd - Chiari syndrome and its clinical significance%布-加综合征患者血清甲胎蛋白水平的检测及其临床意义

    Institute of Scientific and Technical Information of China (English)

    邹文卫; 祖茂衡; 黄光明

    2008-01-01

    目的 探讨布-加综合征(BCS)患者血清甲胎蛋白(AFP)的水平及其临床意义.方法 回顾性分析169例BCS患者的平均血清AFP水平.结果 ①160例单纯性BCS患者的AFP水平为(3.96 4-2.45)μg/L(95%参考值范围:0~8.76μg/L),位于正常人群总体AFP水平区间(0~20μg/L)内.②9例BCS合并肝癌患者AFP水平:2例>1 000μg/L,5例为20~400μg/L,2例<20μg/L.结论 ①单纯BCS患者平均血清AFP水平在正常范围;②合并肝癌的BCS患者血清AFP水平明显高于单纯性BCS患者.

  20. 血清AFP、 GP73和AFU联合检测在HCC中的应用价值%Clinical Value of the Combined Detection of AFP,GP73 and AFU in Patients with HCC

    Institute of Scientific and Technical Information of China (English)

    葛艳玲; 姚艳霞

    2015-01-01

    目的 研究血清甲胎蛋白(AFP)、高尔基体蛋白73(GP73)和α-L-岩藻糖苷酶(AFU)联合检测在原发性肝细胞癌(HCC)诊断中的临床价值.方法 将研究对象分为原发性肝细胞癌(HCC)组,肝硬化组及健康对照组,检测血清AFP、GP73和AFU水平,并对各组结果进行统计学分析.结果 结果显示HCC组AFP、GP73及AFU水平均显著高于其他组,单独检测时血清AFP、GP73和AFU的敏感性分别是52%、74%和55%,特异性分别是93%、95%和88%,联合检测敏感性及特异性分别为93%和86%.结论 AFP、GP73及AFU联合检测可显著提高HCC诊断的敏感性,在HCC的早期诊断中具有重要的临床应用价值.