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Sample records for aloe vera calendula

  1. The Impact of Aloe vera and Calendula on Perineal Healing after Episiotomy in Primiparous Women: A Randomized Clinical Trial

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    Farideh Eghdampour

    2013-11-01

    Full Text Available Introduction: Episiotomy is used for enlarging the perineum. Aloe vera and Calendula have been used for treating different diseases from ancient times, limited researches have been done regarding the healing of these plants. Since the effect of their ointment on episiotomy healing has not been studied, this study is being done for determining the impact of Aloe vera and Calendula on episiotomy healing in primiparous women. Methods: This clinical trial involves 111 qualified primiparous women admitted in Lolagar hospital. They were randomly categorized into three groups of control (n=1 and experimental (n=2 groups. The women in experimental group used Aloe vera and Calendula Ointment every 8 hours and the control group used hospital routine on episiotomy for 5 days. The data were collected by demographic questionnaire and redness, edema, ecchymosis, discharge and approximation scale (REEDA which investigated the episiotomy healing before and five days after intervention in two groups. ANOVA, Tukey test, Kruskal-wallis, Chi-square were used for data analysis. Results: The three groups do not have statistically significant different regarding demographic and other intervening variables. Comparing the mean of REEDA in five days after delivery showed statistically significant difference between control and experimental groups.Conclusion: According to the results, using Aloe vera and Calendula ointment considerably increases the speed of episiotomy wound healing so it can be used for quickening the episiotomy healing.

  2. Aloe vera

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    Klein, A.D.; Penneys, N.S.

    1988-04-01

    We review the scientific literature regarding the aloe vera plant and its products. Aloe vera is known to contain several pharmacologically active ingredients, including a carboxypeptidase that inactivates bradykinin in vitro, salicylates, and a substance(s) that inhibits thromboxane formation in vivo. Scientific studies exist that support an antibacterial and antifungal effect for substance(s) in aloe vera. Studies and case reports provide support for the use of aloe vera in the treatment of radiation ulcers and stasis ulcers in man and burn and frostbite injuries in animals. The evidence for a potential beneficial effect associated with the use of aloe vera is sufficient to warrant the design and implementation of well-controlled clinical trials. 27 references.

  3. Aloe vera on Lanzarote

    OpenAIRE

    Walker, Colin

    2016-01-01

    Aloe vera is the basis of a multibillion dollar cosmetics and toiletries industry worldwide. It is grown commercially in many parts of the world and here its cultivation is described on an aloe farm in the north of Lanzarote, the Canary Islands. The products from such monoculture are sold throughout the island in Aloe vera shops run principally by the company aloe Lanzarote.

  4. Aloe vera in dentistry.

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    Sujatha, G; Kumar, G Senthil; Muruganandan, J; Prasad, T Srinivasa

    2014-10-01

    Aloe vera is a medicinal plant which has been used for thousands of years. The health benefits of aloe vera is well known and the dental uses of this plant is multiple. Interest is gathering among researchers regarding the use of this plant. Studies have proved the antiseptic, anti inflammatory, antiviral and antifungal properties of aloe vera and the use of this plant is proved beneficial. This plant is proved to be non allergic and very good in building up the immune system. Aloe vera is gaining popularity in dentistry as it is completely natural and there is no side effects being reported with its use. This paper gives an overview of the uses of this miracle plant and its uses in dentistry.

  5. Aloe vera: A short review

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    Surjushe Amar

    2008-01-01

    Full Text Available Aloe vera is a natural product that is now a day frequently used in the field of cosmetology. Though there are various indications for its use, controlled trials are needed to determine its real efficacy. The aloe vera plant, its properties, mechanism of action and clinical uses are briefly reviewed in this article.

  6. Aloe Vera for Tissue Engineering Applications

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    Shekh Rahman

    2017-02-01

    Full Text Available Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  7. Aloe Vera for Tissue Engineering Applications.

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    Rahman, Shekh; Carter, Princeton; Bhattarai, Narayan

    2017-02-14

    Aloe vera, also referred as Aloe barbadensis Miller, is a succulent plant widely used for biomedical, pharmaceutical and cosmetic applications. Aloe vera has been used for thousands of years. However, recent significant advances have been made in the development of aloe vera for tissue engineering applications. Aloe vera has received considerable attention in tissue engineering due to its biodegradability, biocompatibility, and low toxicity properties. Aloe vera has been reported to have many biologically active components. The bioactive components of aloe vera have effective antibacterial, anti-inflammatory, antioxidant, and immune-modulatory effects that promote both tissue regeneration and growth. The aloe vera plant, its bioactive components, extraction and processing, and tissue engineering prospects are reviewed in this article. The use of aloe vera as tissue engineering scaffolds, gels, and films is discussed, with a special focus on electrospun nanofibers.

  8. Daya Hambat Ekstrak Aloe Vera terhadap pertumbuhan Staphylococcus Aureus

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    Rahmat, drg.Sp,Pros

    2011-01-01

    Dari hasil penelitian , maka dapat disimpulkan bahwa ekstrak Aloe Vera dapat menghambat pertumbuhan bakteri Stafhylococcus aureus, dan kadar hambat minimal ekstrak Aloe Vera adalah pada konsentrasi 25%. Tujuan Penelitan Ini adalah untuk mengetahui efektifitas ekstrak Aloe vera dalam menghambat pertumbuhan bakteri Stafhylococcus aureus dan daya hambat menimal, (DHM) terhadap pertumbuhan bakteri tersebut. Metode yang digunakan adalah pertumbuhan ekstrak Aloe vera, penegnceran ekstrak , pemur...

  9. Aloe vera in dermatology: a brief review.

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    Feily, A; Namazi, M R

    2009-02-01

    Aloe vera Linne or aloe barbadensis Miller is a succulent from the Aloe family (400 different species), a tropical plant which is easily grown in hot and dry climates and widely distributed in Asia, Africa and other tropical areas. The use of aloe vera is being promoted for a large variety of conditions. The aim of this systematic review was to summarize all dermatology-oriented in vitro and in vivo experiments and clinical trials on aloe vera preparations. Extensive literature search were carried out to identify all in vitro and in vivo studies as well as clinical trials on the subject. Data were extracted from these in a predefined standardized manner. Forty studies were located. The results suggest that oral administration of aloe vera in mice is effective on wound healing, can decrease the number and size of papillomas and reduce the incidence of tumors and leishmania parasitemia by >90% in the liver, spleen, and bone marrow. Topical application of aloe vera is not an effective prevention for radiation-induced injuries and has no sunburn or suntan protection. It can be effective for genital herpes, psoriasis, human papilloma virus, seborrheic dermatitis, aphthous stomatitis, xerosis, lichen planus, frostbite, burn, wound healing and inflammation. It can also be used as a biological vehicle and an anti-microbial and antifungal agent and also as a candidate for photodynamic therapy of some kinds of cancer. Even though there are some promising results with the use of aloe vera for diverse dermatologic conditions, clinical effectiveness of oral and topical aloe vera is not sufficiently and meticulously explored as yet.

  10. Cytotoxicity study of plant Aloe vera (Linn

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    Atul N Chandu

    2012-01-01

    Full Text Available Background: The objective of this study has been to evaluate the in-vitro antitumor activity of Aloe vera extract of in cultured B16F10 melanoma cell line by measuring cell viability using "Trypan blue exclusion assay" method. Aim: To find out such kind of anticancer drug which is a cheap, safe, less toxic, and more potent drug compared to chemotherapy drug. Materials and Methods: In-vitro antitumor activity cell culture1, drug treatment (standard and test extract and Trypan blue exclusion assay growth and viability test 1 were used. Treatment of Aloe vera extract against B16F10 melanoma cell line, in all concentration range, showed decrease in percent cell viability, as compared to that of negative when examined by "Trypan blue exclusion assay". Results: In overall variation of test samples, Aloe vera extract showed its best activity in the concentration of 300 μg/ml, which was approximately equal to the activity of standard drug doxorubicin. Evaluation of in-vitro antitumor activity revealed that Aloe vera extract exhibits good cytotoxic activity. The best cytotoxic activity by Aloe vera was shown at 200 μg/ml concentration. Conclusion: The study of cytoprotection against normal cells by micronucleus assay has shown that the herbal extracts have less toxic effects to the normal blood lymphocytes, as compared to that of standard anticancer drug.

  11. Aloe vera: Plant of Immortality

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    Sikarwar Mukesh. S.

    2010-02-01

    Full Text Available The Egyptians called Aloe the “Plant of Immortality” because it can live and even bloom without soil. Aloe has been used medicinally since at least the first century C.E. and continues to be used extensively worldwide.

  12. Aloe Vera and Infected Leg Ulcers

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    Asima Banu

    2012-06-01

    Full Text Available Background Infected leg ulcers are major health problems resulting in morbidity and disability and are usually chronic and refractory to antimicrobial treatment. Aims The present study is aimed at determining the bacteria involved in leg ulcers and their resistance patterns to commonly used antibiotics as well as to determine whether Aloe Vera has antibacterial activity against multi-drug resistant organisms and promotes wound healing. Method A total of 30 cases with leg ulcers infected with multi-drug resistant organisms were treated with topical aloe vera gel and 30 age and sex-matched controls were treated with topical antibiotics. Culture and sensitivity was done from the wounds on alternate days and the ulcer was clinically and microbiologically assessed after 10 days. The results were compiled and statistically analysed. Results Cultures of the study group who were using aloe vera dressings showed no growth by the fifth day in 10 (33.3% cases, seventh day in another 16 (53.3% and ninth day in two of the remaining four cases (6.7% while in two (6.7% cases there was no decrease in the bacterial count. This means that of the 30 cases, 28 showed no growth by the end of 11 days while two cases showed no decrease in bacterial count. Growth of bacteria in study group is decreased from 100% (30 cases to 6.7% (2 cases by day 11 with P

  13. An evaluation of the biological and toxicological properties of Aloe barbadensis (miller), Aloe vera.

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    Boudreau, Mary D; Beland, Frederick A

    2006-04-01

    Aloe barbadensis (Miller), Aloe vera, has a long history of use as a topical and oral therapeutic. The plant is the source of two products, gel and latex, which are obtained from its fleshy leaves. Aloe vera products contain multiple constituents with potential biological and toxicological activities, yet the active components elude definition. Ingestion of Aloe vera is associated with diarrhea, electrolyte imbalance, kidney dysfunction, and conventional drug interactions; episodes of contact dermatitis, erythema, and phototoxicity have been reported from topical applications. This review examines the botany, physical and chemical properties, and biological activities of the Aloe vera plant.

  14. Qualitative improvement of low meat beef burger using Aloe vera.

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    Soltanizadeh, Nafiseh; Ghiasi-Esfahani, Hossein

    2015-01-01

    Low meat beef burgers have found their niche in the food markets in developing countries because of their lower price. However, these burgers still lack an acceptable quality. This study investigates the effects of different concentrations of Aloe vera on the quality of this food product. For this purpose, beef burgers were produced with 0%, 1%, 3%, and 5% Aloe vera and the changes in their cooking parameters, lipid oxidation, texture, and appeal to consumers over 7days of refrigerated storage were evaluated. Results indicate that Aloe vera contributed to some extent to decreased cooking loss and diameter reduction in the burgers. Increased concentrations of Aloe vera led to improvements in the water absorption and texture of the burgers as well as their lipid stability. However, a concentration level of 3% led to the most acceptability of the product to the panelists. Finally, it was found that Aloe vera acts as a hydrocolloid and improves the quality of burgers.

  15. Of hemorrhagic shock, spherical cows and Aloe vera.

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    Gutierrez, Guillermo; Fuller, Stephanie P

    2004-12-01

    The central question explored in this commentary is whether the beneficial effects of an Aloe vera derived drag-reducing polymer during hemorrhagic shock is due to its O2 radical scavenging properties or to changes in blood rheology.

  16. Aloe vera: A review of toxicity and adverse clinical effects.

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    Guo, Xiaoqing; Mei, Nan

    2016-04-01

    The Aloe plant is employed as a dietary supplement in a variety of foods and as an ingredient in cosmetic products. The widespread human exposure and its potential toxic and carcinogenic activities raise safety concerns. Chemical analysis reveals that the Aloe plant contains various polysaccharides and phenolic chemicals, notably anthraquinones. Ingestion of Aloe preparations is associated with diarrhea, hypokalemia, pseudomelanosis coli, kidney failure, as well as phototoxicity and hypersensitive reactions. Recently, Aloe vera whole leaf extract showed clear evidence of carcinogenic activity in rats, and was classified by the International Agency for Research on Cancer as a possible human carcinogen (Group 2B). This review presents updated information on the toxicological effects, including the cytotoxicity, genotoxicity, carcinogenicity, and adverse clinical effects of Aloe vera whole leaf extract, gel, and latex.

  17. Formulation and Characterization of Aceclofenac -Aloe vera Transemulgel.

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    Raju, Y Prasanna; Haritha, K; Satyanarayana, Rao P; Vandana, K R; Bindu, D Thushara; Vinesha, V; Chowdary, V Harini

    2015-01-01

    The present research was aimed to formulate aceclofenac transemulgel using Aloe vera as gel base. The prepared formulations were subjected to physical characterization, in-vitro and in-vivo assessment. Aceclofenac, a hydrophobic potential non steroidal anti inflammatory drug, causes ulceration upon chronic oral administration, could be formulated into transemulgel to enhance therapeutic efficacy and to lower the unwanted side effects. The transemulgel was prepared from aqueous Aloe vera gel and aceclofenac emulsion. The prepared transemulgel was evaluated for its pH, viscosity, drug content, skin irritation, in-vitro diffusion and accelerated stability studies. The prepared aceclofenac-Aloe vera tranemulgel and commercial aceclofenac gel were subjected to pharmacodynamic studies in albino rats of Wistar strain employing carrageenan induced left hind paw edema method to assess the anti-inflammatory effect. The transemulgel showed a pH of 6.78 and viscosity of 18 cps. In-vitro diffusion data revealed better permeation characteristics. Topical application of formulation found no skin irritation. Stability study has proved the integrity of the formulation. The prepared aceclofenac Aloe vera transemulgel showed better in-vitro drug release when compared with the commercial aceclofenac gel formulation. Anti-inflammatory activity in treated rats showed the significant paw volume reduction at pAloe vera as gel base.

  18. The effects of Aloe vera (Aloe barbadensis) coating on the quality of shrimp during cold storage.

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    Soltanizadeh, Nafiseh; Mousavinejad, Mohsen S

    2015-10-01

    Green tiger shrimp (Penaeus semisulcatus) is an important aquaculture species worldwide. Its perishable nature, however, needs preservation methods to ensure its quality and shelf life. In this study, the effects of Aloe vera coating on the quality and shelf life of shrimps during cold storage were investigated. Shrimp samples were dipped in aqueous solutions containing 25%, 50%, 75%, and 100% Aloe vera gel before storage at 4 °C for 7 days. Drip loss, pH, TBA, TVB-N, and texture of both the control and treated shrimp samples were analyzed periodically. There were significant differences between coated shrimps and the control group in all parameters evaluated. Aloe vera at 75% and 100% concentrations was able to prevent lipid oxidation and drip loss properly; however, coatings containing 25% Aloe vera did not have the desired effects on these characteristics. Shrimps coated with higher concentrations of Aloe vera had better textural properties during cold storage. Results also indicated the positive effects of Aloe vera coating on the sensory quality of shrimp.

  19. Aloe vera for treating acute and chronic wounds

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    Anthony D. Dat

    Full Text Available BACKGROUND: Aloe vera is a cactus-like perennial succulent belonging to the Liliaceae Family that is commonly grown in tropical climates. Animal studies have suggested that Aloe vera may help accelerate the wound healing process.OBJECTIVE: To determine the effects of Aloe vera-derived products (for example dressings and topical gels on the healing of acute wounds (for example lacerations, surgical incisions and burns and chronic wounds (for example infected wounds, arterial and venous ulcers.METHODS:Search methods: We searched the Cochrane Wounds Group Specialised Register (9 September 2011, the Cochrane Central Register of Controlled Trials (CENTRAL (The Cochrane Library 2011, Issue 3, Ovid MEDLINE (2005 to August Week 5 2011, Ovid MEDLINE (In-Process & Other Non-Indexed Citations 8 September 2011, Ovid EMBASE (2007 to 2010 Week 35, Ovid AMED (1985 to September 2011 and EBSCO CINAHL (1982 to 9 September 2011. We did not apply date or language restrictions. Selection criteria: We included all randomised controlled trials that evaluated the effectiveness of Aloe vera, aloe-derived products and a combination of Aloe vera and other dressings as a treatment for acute or chronic wounds. There was no restriction in terms of source, date of publication or language. An objective measure of wound healing (either proportion of completely healed wounds or time to complete healing was the primary endpoint. Data collection and analysis: Two review authors independently carried out trial selection, data extraction and risk of bias assessment, checked by a third review author.MAIN RESULTS:Seven trials were eligible for inclusion, comprising a total of 347 participants. Five trials in people with acute wounds evaluated the effects of Aloe vera on burns, haemorrhoidectomy patients and skin biopsies. Aloe vera mucilage did not increase burn healing compared with silver sulfadiazine (risk ratio (RR 1.41, 95% confidence interval (CI 0.70 to 2.85. A reduction in

  20. Absence of contact sensitization to Aloe vera (L.) Burm. f.

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    Reider, N; Issa, A; Hawranek, T; Schuster, C; Aberer, W; Kofler, H; Fritsch, P; Hausen, B M

    2005-12-01

    Aloe vera has been used as a cosmetic and medical remedy since ancient times and has gained increasing popularity in recent years. Despite its widespread use, reports of allergic reactions are rare. We patch tested 702 consecutive patients with an oily extract from the leaves, Aloe pulvis from the entire plant and concentrated Aloe vera gel. A specially designed questionnaire was used for the use of Aloe vera, reasons and location of application, adverse reactions, occupation, hobbies and atopy. None of the subjects showed any reaction to one of the preparations. 2 components of the plant have to be distinguished: the bark of the leaves contains anthrachinones with pro-peristaltic and potential antibiotic and anticancer properties. Constraints have been imposed due to their considerable toxic potential. Today, mostly the Aloe gel from the center of the leaves is processed. It almost exclusively consists of carbohydrates to which also many medical effects have been attributed. Carbohydrates are not likely to induce contact sensitization, which might explain the outcome of our study. However, this does not justify unrestrained promotion of Aloe products, as scientific studies investigating the claims on its constitutional effects are few in number, and the majority of them have been unable to diminish the intuitive scepticism against miracle cures, like Aloe seems to be.

  1. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE;

    2015-01-01

    Background: Aloe vera supports a substantial global trade yet its wild origins, and explanations for its popularity over 500 related Aloe species in one of the world’s largest succulent groups, have remained uncertain. We developed an explicit phylogenetic framework to explore links between...... the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...... succulence among aloes has yielded new explanations for the extraordinary market dominance of Aloe vera. The industry preference for Aloe vera appears to be due to its proximity to important historic trade routes, and early introduction to trade and cultivation. Well-developed succulent leaf mesophyll tissue...

  2. ALOE VERA: A REVIEW OF ITS CLINICAL EFFECTIVENESS

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    Malik Itrat

    2013-08-01

    Full Text Available Aloe vera has been used for over five thousand years. Throughout history, it has been considered a magical plant, almost a panacea, capable of remedying many of mankind’s ailments. It is only in the last 20 years, after a series of proven research, that we can highlight the characteristics of this plant, whose secrets have been hidden behind a blanket of botanical and pharmacological puzzles that only today begin to yield some answers. The Aloe vera plant and its clinical uses are briefly reviewed in this article.

  3. Aloe vera as a functional ingredient in foods.

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    Rodríguez Rodríguez, Elena; Darias Martín, Jacinto; Díaz Romero, Carlos

    2010-04-01

    The main scientific discoveries on Aloe vera published mainly in the last three decades are presented in this work. After describing Aloe from a botanical point of view, the papers related with the chemical composition of different parts of the leaf of Aloe, particularly those in which the gel is described and are presented in a synthetic manner. The chemical analyses reveal that Aloe gel contains mannose polymers with some glucose and other sugars, among which the most important is Acemannan. Besides these, other components such as glycoproteins, enzymes, amino acids, vitamins, and minerals are described. Different factors also affecting the chemical composition of the gel, such as species and variety, climatic and soil conditions, cultivation methods, processing and preservation, are enumerated and discussed. On the other hand, the main therapeutic applications have been revised and the possible damaging effects of Aloe are also commented upon. A special emphasis is placed on the biologically active compounds or groups of compounds responsible for the therapeutic applications and which are their action mechanisms. The paper concludes that more research is needed to confirm the therapeutic and beneficial effects and to definitively clarify the myth surrounding Aloe vera. A general view on the problem of the commercialization and establishment of the quality and safety of Aloe products in the food industry has been offered here. The main points and European regulations that need to be considered regarding the quality control of prepared Aloe products are presented in this paper.

  4. Separation of Water to Concentrate Aloe Vera Juice

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    Ishfaq Abdullah

    2014-11-01

    Full Text Available Aloe Vera is a succulent plant and is found in different arid areas. Due to its healing effect and soothing properties it is being used in herbal medicines for years and its importance has grown due to use in cosmetic products. It contains more than thirty active ingredients, which are utilized in cosmetic and pharmaceutical industry. It also contains 98% water. To remove water from those active ingredient Reverse osmosis is used in order not to thermally damage the active ingredients present in the juice. A reverse osmosis is a separation technique which utilizes difference in pressure to segregate water from plant without damaging the active ingredients present in the plant. This research is dedicated to evaluate the parameter for separation of water from aloe Vera so that aloe Vera can be obtained in a form which can be utilized for different purposes such as cosmetics and medicine. Osmosis technique is preferred over other conventional technique for the separation purpose because it is not operated at high temperature, which will not damage the aloe Vera juice quality. Next step in this research is the preserving, drying and analysis of this juice so that this product can be utilized in versatile way.

  5. Immunomodulatory properties of aloe vera gel in mice

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    Madan Jyotsana

    2008-01-01

    Full Text Available Administration of Aloe vera extract to swiss albino mice (300 mg/kg i.p. daily for five days, significantly (P < 0.01 increases the total white blood cells count. Further, it increases humoral immune response, as demonstrated from the increase in plaque-forming cells in the spleen and circulating antibody titre.

  6. Oral Aloe vera as a treatment for osteoarthritis: a summary.

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    Cowan, David

    2010-06-01

    While pain relief is a basic tenet of health care, pain is under-treated in the UK (Davies and Mcvicar, 2000) and this issue remains unresolved. This paper suggests that oral Aloe vera could be used in the treatment of chronic non-cancer pain (CNCP), particularly that caused by osteoarthritis (OA). Despite being used as arthritis treatment for centuries (Yoo et al, 2008), evidence of effectiveness of Aloe vera is anecdotal or from small studies. The perceived benefits of prescribing Aloe vera for OA may be twofold: it has utility as an anti-inflammatory agent and also as a prophylactic against the gastrointestinal irritant effects of non-steroidal anti-inflammatory drugs (NSAIDs). Long-term, randomized, controlled studies are still needed to address the lack of evidence informing optimum prescribing of pain medication for people with OA (Cowan, 2007). There is no reason that so called 'nutraceutical' agents should not be subjected to the same rigorous randomized, controlled, double-blind trials as other 'mainstream' drugs. Therefore, it is appropriate to ask whether NSAID treatment and side effects can be improved by the addition of oral Aloe vera. Thus, we may then be in a more informed position to resolve the ongoing 'Pandemonium over Painkillers' (Cowan, 2007).

  7. In vitro and in vivo antioxidant activities of polysaccharide purified from aloe vera (Aloe barbadensis) gel.

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    Kang, Min-Cheol; Kim, Seo Young; Kim, Yoon Taek; Kim, Eun-A; Lee, Seung-Hong; Ko, Seok-Chun; Wijesinghe, W A J P; Samarakoon, Kalpa W; Kim, Young-Sun; Cho, Jin Hun; Jang, Hyeang-Su; Jeon, You-Jin

    2014-01-01

    The in vitro and in vivo antioxidant potentials of a polysaccharide isolated from aloe vera gel were investigated. Enzymatic extracts were prepared from aloe vera gel by using ten digestive enzymes including five carbohydrases and five proteases. Among them, the highest yield was obtained with the Viscozyme extract and the same extract showed the best radical scavenging activity. An active polysaccharide was purified from the Viscozyme extract using ethanol-added separation and anion exchange chromatography. Purified aloe vera polysaccharide (APS) strongly scavenged radicals including DPPH, hydroxyl and alkyl radicals. In addition, APS showed a protective effect against AAPH-induced oxidative stress and cell death in Vero cells as well as in the in vivo zebrafish model. In this study, it is proved that both the in vitro and in vivo antioxidant potentials of APS could be further utilized in relevant industrial applications.

  8. Topical Aloe Vera (Aloe barbadensis Miller) Extract Does Not Accelerate the Oral Wound Healing in Rats.

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    Coelho, Fernanda Hack; Salvadori, Gabriela; Rados, Pantelis Varvaki; Magnusson, Alessandra; Danilevicz, Chris Krebs; Meurer, Luise; Martins, Manoela Domingues

    2015-07-01

    The effect of topical application of Aloe Vera (Aloe barbadensis Miller) extract was assessed on the healing of rat oral wounds in an in vivo model using 72 male Wistar rats divided into three groups (n = 24): control, placebo and Aloe Vera (0.5% extract hydroalcoholic). Traumatic ulcers were caused in the dorsum of the tongue using a 3-mm punch tool. The Aloe Vera and placebo group received two daily applications. The animals were sacrificed after 1, 5, 10 and 14 days. Clinical analysis (ulcer area and percentage of repair) and histopathological analysis (degree of re-epithelialization and inflammation) were performed. The comparison of the differences between scores based on group and experimental period, both in quantitative and semi-quantitative analyses, was performed using the Kruskal-Wallis test. The significance level was 5%. On day 1, all groups showed predominantly acute inflammatory infiltrate. On day 5, there was partial epithelialization and chronic inflammatory infiltrate. On the days 10 and 14 total repair of ulcers was observed. There was no significant difference between groups in the repair of mouth ulcers. It is concluded that treatment using Aloe Vera as an herbal formulation did not accelerate oral wound healing in rats.

  9. SINTESIS MEMBRAN NATA ALOE VERA-ETILENDIAMIN DAN KARAKTERISASINYA

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    EB Susatyo

    2014-06-01

    Full Text Available Serat yang terkandung di dalam nata Aloe vera adalah selulosa sehingga dapat digunakan dalam sintesis membran. Telah dilakukan penelitian tentang sintesis membran nata Aloe vera-etilendiamin (nata-en menggunakan sistem vakum cair. Proses preparasi membran berlangsung melalui tiga tahap, yaitu preparasi nata Aloe vera, aktivasi menggunakan asam sulfat, dan modifikasi dengan menggunakan etilendiamin. Tujuan penelitian adalah untuk mempelajari teknik dalam sintesis membran nata-en kemudian melakukan karakterisasi untuk mengetahui karakter strukturnya. Hasil penelitian menunjukkan perilaku yang berbeda dalam hal sifat mekanik dan strukturnya. Membran nata murni memiliki sifat mekanik yang kuat, nata teraktivasi cenderung rapuh, sedangkan nata-en bersifat liat. Spektra infra merah dari ketiga tipe membran (nata murni, nata teraktivasi, dan nata-en secara umum tidak mengalami perubahan yang signifikan, hanya terjadi pergeseran panjang gelombang dari masing-masing membran. Berdasarkan spektra infra merah dapat diketahui bahwa masing-masing membran mempunyai gugus hidroksil, tetapi serapannya semakin melebar untuk setiap membran. Gugus alkil dan karboksil juga masih tampak, namun pada membran nata teraktivasi serapannya berkurang, sedangkan pada membran nata-en muncul puncak baru yang menunjukkan adanya gugus amin. Hal ini membuktikan bahwa telah terjadi ikatan antara nata dengan etilendiamin. Fibers contained in nata Aloe vera is cellulose that can be used in the synthesis of membrane. The research has done on the synthesis of nata Aloe vera-ethylenediamine (nata-en membrane by using liquid vacuum system. Membrane preparation process consisted of three stages, they are nata Aloe vera preparation, activation using sulfuric acid, and membrane modification by using ethylenediamine. The purpose of research is to study the technique of nata-en membrane synthesis then to perform the characterization to determine the character of their structure.The results

  10. The Review on Properties of Aloe Vera in Healing of Cutaneous Wounds.

    Science.gov (United States)

    Hashemi, Seyyed Abbas; Madani, Seyyed Abdollah; Abediankenari, Saied

    2015-01-01

    Treatment of wounds is very important and was subject of different investigations. In this regard, natural substance plays crucial role as complementary medicine. Various studies reported that aloe vera has useful effects on wounds especially cutaneous wounds healing. Therefore in the current review, we examined the effect of aloe vera on cutaneous wound healing and concluded that although aloe vera improves the wound healing as well as other procedures both clinically and experimentally, more studies are still needed to approve the outcomes.

  11. Effectivity of Aloe vera bioactives as feed additive for broilers reared on deep litter

    OpenAIRE

    A.P Sinurat; T Purwadaria; T Pasaribu; W Rakhmani; J Dharma; J Rosida; S Sitompul; Udjianto

    2004-01-01

    There are plenty of Indonesian plants contain usefull bioactive components. One of them is Aloe vera. Previous experiment showed that Aloe vera bioactives reduced aerob bacteria in the intestinal and improved feed efficiency in broilers reared in cages. The results however, gave some variations, may be due to variation in rearing the chickens. Two experiments were carried out to study the effectivity of Aloe vera bioactives as feed additives for broilers reared on deep litter. In the first st...

  12. The effect of Aloe vera bioactive and anthraquinone on the performance of laying hens

    OpenAIRE

    Tiurma Pasaribu; A.P Sinurat; Susana I.W Rakhman

    2005-01-01

    An experiment was conducted to study the use of Aloe vera bioactives as feed additives on the performances of laying hens. The Aloe vera bioactives was prepared as the dry gel (DG) and semi liquid gel (SLG). The Aloe vera was suplemented into the diets with concentration of equal to 0.5 and 1.0 g DG/kg diets. Diets contained commercial anthraquinone, a bioactive compound of Aloe vera with doses equal to 0.5 g DG and 1.0 dg/kg were also prepared. Diets were compared to control diets containing...

  13. Development of Aloe vera based edible coating for tomato

    Science.gov (United States)

    Athmaselvi, K. A.; Sumitha, P.; Revathy, B.

    2013-12-01

    The effect of formulated Aloe vera based edible coating on mass loss, colour, firmness, pH, acidity, total soluble solid, ascorbic acid and lycopene on the coated tomato was investigated. The tomato in control showed a rapid deterioration with an estimated shelf life period of 19 days, based on the mass loss, colour changes, accelerated softening and ripening. On the contrary, the coating on tomatoes delayed the ripening and extended the shelf life up to 39 days. The physiological loss in weight was 7.6 and 15.1%, firmness was 36 and 46.2 N on 20th day for control and coated tomatoes, respectively. From the results, it was concluded that the use of Aloe vera based edible coating leads to increased tomato shelf-life.

  14. Memristors in the electrical network of Aloe vera L.

    Science.gov (United States)

    Volkov, Alexander G; Reedus, Jada; Mitchell, Colee M; Tucket, Clayton; Forde-Tuckett, Victoria; Volkova, Maya I; Markin, Vladislav S; Chua, Leon

    2014-01-01

    A memristor is a resistor with memory, which is a non-linear passive two-terminal electrical element relating magnetic flux linkage and electrical charge. Here we found that memristors exist in vivo. The electrostimulation of the Aloe vera by bipolar sinusoidal or triangle periodic waves induce electrical responses with fingerprints of memristors. Uncouplers carbonylcyanide-3-chlorophenylhydrazone and carbonylcyanide-4-trifluoromethoxy-phenyl hydrazone decrease the amplitude of electrical responses at low and high frequencies of bipolar periodic sinusoidal or triangle electrostimulating waves. Memristive behavior of an electrical network in the Aloe vera is linked to the properties of voltage gated ion channels: the K(+) channel blocker TEACl reduces the electric response to a conventional resistor. Our results demonstrate that a voltage gated K(+) channel in the excitable tissue of plants has properties of a memristor. The discovery of memristors in plants creates a new direction in the modeling and understanding of electrical phenomena in plants.

  15. Encapsulation of Aloe Vera and Its Effect During Yogur Incubation

    Directory of Open Access Journals (Sweden)

    Ricardo Adolfo Parra Huertas

    2014-11-01

    Full Text Available The yogurt is milk derivative highly consumed around the world,as well as aloe vera. Both have reports tocontribute to human health. The purpose of this research is to determine the effect of the addition of capsules with aloe vera during the incubation of yogurt. Aloeverawas encapsulated in alginate at two different concentrations, 1% and 2%,addingthe capsules from the moment of incubation and comparing the effect of the addition of capsules withthe non-addition of them. For these samples were determined: pH, acidity, syneresis, lactic acid bacteria count, sensory evaluation and proximate analysis. The results indicated that for the three treatments pH values and acid behaved similarly to each characteristic of the yogurt during incubation. The lactic acid bacteria count indicated that treatment with capsules containing 2% sodium alginate had higher counts. Sensorially, three treatments had a favorable acceptability; proximate analysis had favorable values . In conclusion,the tests showed the viability of encapsulated aloe vera in the manufacture of yogurt during incubation time without being affected by the concentration of sodium alginate.

  16. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress.

    Science.gov (United States)

    Salinas, Carlos; Handford, Michael; Pauly, Markus; Dupree, Paul; Cardemil, Liliana

    2016-01-01

    Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  17. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera Grown under Water Stress.

    Directory of Open Access Journals (Sweden)

    Carlos Salinas

    Full Text Available Aloe barbadensis Miller (Aloe vera has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC. There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported.

  18. Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress

    Science.gov (United States)

    Salinas, Carlos; Cardemil, Liliana

    2016-01-01

    Aloe barbadensis Miller (Aloe vera) has a Crassulaceae acid metabolism which grants the plant great tolerance to water restrictions. Carbohydrates such as acemannans and fructans are among the molecules responsible for tolerating water deficit in other plant species. Nevertheless, fructans, which are prebiotic compounds, have not been described nor studied in Aloe vera, whose leaf gel is known to possess beneficial pharmaceutical, nutritional and cosmetic properties. As Aloe vera is frequently cultivated in semi-arid conditions, like those found in northern Chile, we investigated the effect of water deficit on fructan composition and structure. For this, plants were subjected to different irrigation regimes of 100%, 75%, 50% and 25% field capacity (FC). There was a significant increase in the total sugars, soluble sugars and oligo and polyfructans in plants subjected to water deficit, compared to the control condition (100% FC) in both leaf tips and bases. The amounts of fructans were also greater in the bases compared to the leaf tips in all water treatments. Fructans also increase in degree of polymerization with increasing water deficit. Glycosidic linkage analyses by GC-MS, led to the conclusion that there are structural differences between the fructans present in the leaves of control plants with respect to plants irrigated with 50% and 25% FC. Therefore, in non-stressed plants, the inulin, neo-inulin and neo-levan type of fructans predominate, while in the most stressful conditions for the plant, Aloe vera also synthesizes fructans with a more branched structure, the neofructans. To our knowledge, the synthesis and the protective role of neo-fructans under extreme water deficit has not been previously reported. PMID:27454873

  19. Bacterial leaf rot of Aloe vera L., caused byErwinia chrysanthemi biovar 3

    NARCIS (Netherlands)

    Laat, de P.C.A.; Verhoeven, J.T.W.; Danse, J.D.

    1994-01-01

    A severe attack of the bacteriumErwinia chrysantemi biovar 3 on the succulentAloe vera on the Carribean island of Aruba is described. Biochemical and pathological characteristics of strains are presented, including results of successful inoculation experiments onAloe vera. This is the first report o

  20. Processing, food applications and safety of aloe vera products: a review.

    Science.gov (United States)

    Ahlawat, Kulveer Singh; Khatkar, Bhupender Singh

    2011-10-01

    Aloe vera is used for vigor, wellness and medicinal purposes since rigvedic times. Health benefits of aloe vera include its application in wound healing, treating burns, minimizing frost bite damage, protection against skin damage from x-rays, lung cancer, intestinal problems, increasing high density lipoprotein (HDL), reducing low density lipoprotein (LDL), reducing blood sugar in diabetics, fighting acquired immune deficiency syndrome (AIDS), allergies and improving immune system. Phytochemistry of aloe vera gel has revealed the presence of more than 200 bioactive chemicals. Aloe vera gel is extracted from its leaves and appropriate processing techniques are needed for stabilization as well as preparation of the end products. The industries involved in processing of aloe vera need Government surveillance to ensure that the aloe vera products have beneficial bio-active chemicals as per claims of the manufacturers. Regulatory bodies also need to look into the safety and toxicological aspects of aloe vera products for food applications. The claims made for medicinal value of aloe products should be supported by authentic and approved clinical trial data. It is presumptive to mention that nutraceutical claims of aloe products made by the manufacturers are numerous. However, approved clinical evidences are available only for lowering LDL, increasing HDL, decreasing blood glucose level, treating genital herpes and psoriases.

  1. Inhibitory activity of Aloe vera gel on some clinically isolated cariogenic and periodontopathic bacteria.

    Science.gov (United States)

    Fani, Mohammadmehdi; Kohanteb, Jamshid

    2012-03-01

    Aloe vera is a medicinal plant with anti-inflammatory, antimicrobial, antidiabetic and immune-boosting properties. In the present study we investigated the inhibitory activities of Aloe vera gel on some cariogenic (Streptococcus mutans), periodontopathic (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis) and an opportunistic periodontopathogen (Bacteroides fragilis) isolated from patients with dental caries and periodontal diseases. Twenty isolates of each of these bacteria were investigated for their sensitivity to Aloe vera gel using the disk diffusion and microdilution methods. S. mutans was the species most sensitive to Aloe vera gel with a MIC of 12.5 µg/ml, while A. actinomycetemcomitans, P. gingivalis, and B. fragilis were less sensitive, with a MIC of 25-50 µg/ml (P Aloe vera gel at optimum concentration could be used as an antiseptic for prevention of dental caries and periodontal diseases.

  2. Phenolic constituents in dried flowers of aloe vera (Aloe barbadensis) and their in vitro antioxidative capacity.

    Science.gov (United States)

    Keyhanian, Shirin; Stahl-Biskup, Elisabeth

    2007-06-01

    The dried flowers from Aloe vera (L.) Burm. f. (Aloe barbadensis Mill.) (Asphodelaceae) were analysed by means of HPLC-DAD and HPLC-MS/MS, verifying chlorogenic, caffeic, 5-P-coumaroylquinic, caffeoylshikimic, 5-feruloylquinic, 5-P-CIS-coumaroylquinic, P-coumaric and ferulic acid as well as luteolin, apigenin, quercetin, kaempferol, isoorientin, isovitexin and their 7-O-glucosides, saponarin and lutonarin. On searching for anthranoids in the flower extract, aloe-emodin as well as the glycosylchromone aloeresin B could be identified. Aloin A and B, the laxative principle of the drug Curaçao-Aloes, are not accumulated in the dried flowers. The polyphenol content of three different batches was 0.73 - 1.01% (+/- 0.05%) and the flavonoid content 0.24 - 0.34% (+/- 0.01%). The hydrophilic antioxidative capacity amounted to 85.7 - 94.9 (+/- 0.5) micromol TEAC/g dried Aloe vera flower and was directly correlated with the polyphenol and flavonoid contents.

  3. Assessment of Anti HSV-1 Activity of Aloe Vera Gel Extract: an In Vitro Study

    Science.gov (United States)

    Rezazadeh, Fahimeh; Moshaverinia, Maryam; Motamedifar, Mohammad; Alyaseri, Montazer

    2016-01-01

    Statement of the Problem Herpes simplex virus (HSV) infection is one of the most common and debilitating oral diseases; yet, there is no standard topical treatment to control it. The extract of Aloe vera leaves has been previously reported to have anti-inflammatory, antibacterial, and also antiviral effects. There is no data on anti-Herpes simplex virus type 1 (HSV-1) activity of Aloe vera gel. Purpose This study aimed to evaluate the anti-HSV-1 activity of Aloe vera gel in Vero cell line. Materials and Method In this study, gel extraction and cytotoxicity of various increasing concentrations of Aloe vera gel (0.2, 0.5, 1, 2, and 5%) was evaluated in Dulbecco’s Modified Eagle Medium (DMEM) containing 2% fetal bovine serum (FBS). Having been washed with phosphate buffered saline, 50 plaque-forming units (PFU) of HSV-1 was added to each well. After 1 hour of incubation at 37°C, cell monolayers in 24 well plates were exposed to different increasing concentrations of Aloe vera gel. The anti-HSV-1 activity of Aloe vera gel in different concentrations was assessed by plaque reduction assays. Data were analyzed by using One-way ANOVA. Results The cytotoxicity assay showed that Aloe vera in prearranged concentrations was cell-compatible. The inhibitory effect of various concentrations of Aloe vera was observed one hour after the Vero cell was infected with HSV-1. However, there was no significant difference between two serial concentrations (p> 0.05). One-way ANOVA also revealed no significant difference between the groups. The findings indicated a dose-dependent antiviral effect of Aloe vera. Conclusion The findings showed significant inhibitory effect of 0.2-5% Aloe vera gel on HSV-1 growth in Vero cell line. Therefore, this gel could be a useful topical treatment for oral HSV-1 infections without any significant toxicity. PMID:26966709

  4. Aloe vera-based formula as emollient on horses' hooves

    Directory of Open Access Journals (Sweden)

    Samuel Pereira Simonato

    2013-06-01

    Full Text Available The present study aimed at developing an Aloe vera-based formula for topical use on horse hoof and evaluating whether the treatment affects hooves growth and balance. Six healthy male horses between the ages of 3 and 17 years (12±5.25 were used, all semi-confined animals for breeding purposes. Before beginning A. vera treatment, animals underwent two trimming procedures with a 45 days-interval. After the second trimming, one of the forelimbs and one of the hindlimbs of 4 horses was weekly treated by topical application of the glycolic extract of A. vera at 20%. The contralateral limb, randomly chosen, received the extract at 50%. The hooves of the other animals were treated with propylene glycol. Treatment was done for 225 days and, during this time, animals underwent periodic trimming. Variables related to growth and balance of the hooves were measured before and after trimming. Data were analyzed using chi-square test and regression analysis at 5% significance. Growth rate of the hooves was not related to treatment. On the other hand, the 50% extract was related to the majority of the hooves in balance (p<0.05. Results suggest that a weekly topical treatment with A. vera glycolic extract does not improve the growth rate of the hooves; however, when applied at a high concentration, it improves their balance.

  5. A review on ethnopharmacological potential of Aloe vera L.

    Directory of Open Access Journals (Sweden)

    Md. Shamim Hossain

    2013-04-01

    Full Text Available In recent years, Aloe vera Linn. (Ghritokumari locally has become a subject of interest because of its beneficial effects on human health. The present ethnopharmacological review was conducted to evaluate the therapeutic properties of A. vera by scientific evidences. It belongs to the family Liliaceae, is a perennial herb with 30-60cm long juicy leaves which is found all over Bangladesh. To date, more than 75 active ingredients including aloesin, aloeemodin, acemannan, aloeride, methylchromones, flavonoids, saponin, amino acids, vitamins, and minerals have been identified from inner gel of leaves. It has antiinflammatory, antioxidant, antimicrobial, anticancer, antidiabetic, immuneboosting, and hypoglycemic properties. Daily supplementation with this is effective against stroke, heart attacks, leukemia, anemia, hypertension, AIDS, radiation burns, digestive disorders etc. This study also covers its taxonomy, distribution, morphology, and monograph. [J Intercult Ethnopharmacol 2013; 2(2.000: 113-120

  6. Electrical signaling in Aloe vera induced by localized thermal stress.

    Science.gov (United States)

    Volkov, Alexander G; Lang, Ryan D; Volkova-Gugeshashvili, Maia I

    2007-11-01

    Action potentials in higher plants are theorized as the information carriers in intercellular and intracellular communication in the presence of environmental stressors. Among the most common stressors is heat shock. Under stressful conditions, the response reactions of plant tissues and organs can be local or transmitted over long distances. In this article, the speeds of propagation of thermally induced action potentials in green plants are discussed, and their speeds were found to be comparable to those occurring in various mammalian species. These rapid action potentials in green plants were recorded in real time using modern data acquisition techniques. According to our measurements, a single application of localized heat stress induces fast action potentials in Aloe vera (67 m/s). Electrical signals propagated along all leaves of the A. vera plants were studied. Possible pathways for electrical signal propagation in vascular plants are also discussed.

  7. Comparative efficacy of aloe vera mouthwash and chlorhexidine on periodontal health: A randomized controlled trial

    Science.gov (United States)

    Jha, Abhishek; Bhashyam, Mamtha

    2016-01-01

    Background With introduction of many herbal medicines, dentistry has recently evidenced shift of approach for treating many inflammatory oral diseases by using such modalities. Aloe vera is one such product exhibiting multiple benefits and has gained considerable importance in clinical research recently. Aim To compare the efficacy of Aloevera and Chlorhexidine mouthwash on Periodontal Health. Material and Methods Thirty days randomized controlled trial was conducted among 390 dental students. The students were randomized into two intervention groups namely Aloe Vera (AV) chlorhexidine group (CHX) and one control (placebo) group. Plaque index and gingival index was recorded for each participant at baseline, 15 days and 30 days. The findings were than statistically analyzed, ANOVA and Post Hoc test were used. Results There was significant reduction (pAloe Vera (AV) and chlorhexidine group. Post hoc test showed significant difference (paloe Vera and placebo and chlorhexidine and placebo group. No significant difference (pAloe vera, chlorhexidine, dental plaque, gingivitis. PMID:27703614

  8. The efficacy of aloe vera used for burn wound healing: a systematic review.

    Science.gov (United States)

    Maenthaisong, Ratree; Chaiyakunapruk, Nathorn; Niruntraporn, Surachet; Kongkaew, Chuenjid

    2007-09-01

    Aloe vera has been traditionally used for burn healing but clinical evidence remains unclear. We conducted a systematic review to determine the efficacy of topical aloe vera for the treatment of burn wounds. We electronically searched relevant studies in MEDLINE, CINAHL, Cochrane Library, HealthSTAR, DARE, South-East Asia Database, Chinese Databases, and several Thai local Databases (1918-June 2004). Only controlled clinical trials for burn healing were included. There were no restrictions on any language of publication. Two reviewers independently extracted data on study characteristics, patient characteristics, intervention, and outcome measure. Four studies with a total of 371 patients were included in this review. Based on a meta-analysis using duration of wound healing as an outcome measure, the summary weighted mean difference in healing time of the aloe vera group was 8.79 days shorter than those in the control group (P=0.006). Due to the differences of products and outcome measures, there is paucity to draw a specific conclusion regarding the effect of aloe vera for burn wound healing. However, cumulative evidence tends to support that aloe vera might be an effective interventions used in burn wound healing for first to second degree burns. Further, well-designed trials with sufficient details of the contents of aloe vera products should be carried out to determine the effectiveness of aloe vera.

  9. Quality change of apple slices coated with Aloe vera gel during storage.

    Science.gov (United States)

    Song, Hye-Yeon; Jo, Wan-Shin; Song, Nak-Bum; Min, Sea C; Song, Kyung Bin

    2013-06-01

    Fresh-cut apples are easily susceptible to browning and microbial spoilage. In this study, an edible coating prepared from Aloe vera gel containing antibrowning solution was applied to preserve the quality of fresh-cut apples during storage. Fresh-cut apples were treated with both an Aloe vera gel and an Aloe vera gel containing 0.5% cysteine and then stored at 4 °C for 16 d. The color, firmness, weight loss, soluble solid content, titratable acidity, microbial analysis, and sensory evaluation were analyzed during storage. Fresh-cut apples coated with the Aloe vera gel showed delayed browning and reduced weight loss and softening compared to the control. The Aloe vera gel coating was also effective in reducing the populations of the total aerobic bacteria and yeast and molds. In particular, Aloe vera gel containing 0.5% cysteine was most effective in delaying browning and the reduction of microbial populations among the treatments. These results suggest that an Aloe vera gel coating can be used for maintaining the quality of fresh-cut apples.

  10. Propagation Techniques and Agronomic Requirements for the Cultivation of Barbados Aloe (Aloe vera (L.) Burm. F.)—A Review

    Science.gov (United States)

    Cristiano, Giuseppe; Murillo-Amador, Bernardo; De Lucia, Barbara

    2016-01-01

    Barbados aloe (Aloe vera (L.) Burm. F.) has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic, and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe, and America. In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems. PMID:27721816

  11. PROPAGATION TECHNIQUES AND AGRONOMIC REQUIREMENTS FOR THE CULTIVATION OF BARBADOS ALOE (ALOE VERA (L. BURM. F. - A REVIEW.

    Directory of Open Access Journals (Sweden)

    Barbara De Lucia

    2016-09-01

    Full Text Available Barbados aloe (Aloe vera (L. Burm. f. has traditionally been used for healing in natural medicine. However, aloe is now attracting great interest in the global market due to its bioactive chemicals which are extracted from the leaves and used in industrial preparations for pharmaceutical, cosmetic and food products. Aloe originated from tropical and sub-tropical Africa, but it is also now cultivated in warm climatic areas of Asia, Europe and America.In this review, the most important factors affecting aloe production are described. We focus on propagation techniques, sustainable agronomic practices and efficient post harvesting and processing systems.

  12. DNA degradation by aqueous extract of Aloe vera in the presence of copper ions.

    Science.gov (United States)

    Naqvi, Shoa; Ullah, M F; Hadi, S M

    2010-06-01

    The plant Aloe vera has long been used in medicine, as dietary supplements and for cosmetic purposes. Aloe vera extracts are a rich source of polyphenols, such as aloin and aloe emodin and have shown a wide range of pharmacological properties, including anti-inflammatory and anti-cancer properties. The bioactive component aloe emodin has been reported to induce apoptosis in various cancer cell lines. Many of the biological activities of Aloe vera have been attributed to its antioxidant properties. However, most plant-derived polyphenols that are also present in Aloe vera may exhibit pro-oxidant properties either alone or in the presence of transition metals, such as copper. Previous reports from this laboratory have implicated the pro-oxidant action as one of the mechanisms for their anti-cancer properties. In the present paper, we show that aqueous extract of Aloe vera is also able to cause DNA degradation in the presence of copper ions. Further, the extract is also able to reduce Cu(II) to Cu(I) and generate reactive oxygen species, such as superoxide anion and hydroxyl radicals in a dose-dependent manner, which correlates with ability of the extract to cause DNA breakage. Thus, the study shows that in addition to antioxidant activity, Aloe vera extract also possess pro-oxidant properties, leading to oxidative DNA breakage.

  13. Acemannan, an extracted polysaccharide from Aloe vera: A literature review.

    Science.gov (United States)

    Sierra-García, Gerardo Daniel; Castro-Ríos, Rocío; González-Horta, Azucena; Lara-Arias, Jorge; Chávez-Montes, Abelardo

    2014-08-01

    In this review, the composition, actions, and clinical applications of acemannan in medicine and its effectiveness as an adjunct in the treatment of diseases are presented. An electronic literature search was performed up to January 2014 for studies and research presenting data to validate the efficacy of acemannan. A total of 50 titles, abstracts and full-text studies were selected and reviewed. Acemannan has various medicinal properties like osteogenic, anti-inflammatory, and antibacterial, which accelerate healing of lesions. Also, acemannan is known to have antiviral and antitumor activities in vivo through activation of immune responses. It was concluded that Aloe vera has immense potential as a therapeutic agent. Even though the plant is a promising herb with various clinical applications in medicine and dentistry, more clinical research needs to be undertaken to validate and explain the action of acemannan in healing, so that it can be established in the field of medicine and a more precise understanding of the biological activities of these is required to develop Aloe vera as a pharmaceutical source.

  14. ANTIOXIDANT EFFECT OF ALOE VERA IN EXPERIMENTALLY INDUCED DIABETES MELLITUS

    Directory of Open Access Journals (Sweden)

    Jyoti Sethi et al

    2012-08-01

    Full Text Available The aim of the present study was to evaluate antidiabetic and antioxidant potential of Aloe vera (AV in alloxan induced diabetes in albino rabbits. Experimental Diabetes was induced in rabbits with alloxan(80mg/Kg body weightand animals showing fasting blood glucose levels more than 250mg/dl were considered as diabetics and divided into four groups of six each (n=6. Group I: Normal control rabbits, Group II: Alloxan induced diabetic rabbits, Group III: Diabetic rabbits received AV gel extract (300 mg/Kg in aqueous solution for 21 days, Group IV: diabetic rabbits given glibenclamide (600ug/kg in aqueous solution. All the drugs were administered orally (using an intra gastric tube in a single dose in the morning for 21 days. Blood samples were collected from the marginal vein of pinna of overnight fasted rabbits (Blood sugar, glycosylated hemoglobin (HbA1c, Malondialdehyde (MDA, reduced glutathione (GSH, total thiols (PSH and Superoxide dismutase (SOD. Oral administration of AV showed potent antihyperglycemic and anti-lipidperoxidative effect in diabetic animals. Simultaneously, the levels of protective antioxidant enzymes (SOD, GSH and PSH were significantly increased with AV supplementation. The results suggest potent antidiabetic and antioxidant potential of AV in experimental diabetes, and thus Aloe vera can be used as an alternative remedy for treatment of diabetes mellitus and its complications.

  15. Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens.

    Science.gov (United States)

    Yim, Dongjean; Kang, Sang S; Kim, Dong W; Kim, Sang H; Lillehoj, Hyun S; Min, Wongi

    2011-01-01

    Aloes have been widely used for a broad range of pharmacological activities, including parasitic problems. Avian coccidiosis is the most costly and wide-spread parasitic disease in the poultry industry, and has been mainly controlled by the use of chemotherapeutic agents. Due to the emergence of drug-resistant strains, alternative control strategies are needed. In this study, the protective effects of Aloe vera-based diets were assessed in broiler chickens following oral infection with Eimeria maxima. Chickens were fed a regular diet supplemented with ground Aloe vera throughout the duration of the experiment beginning 2 days prior to infection with 1 × 10(4) sporulated oocysts of E. maxima. No significant differences were found in body weight gain or loss between the Aloe vera-supplemented and unsupplemented groups with or without E. maxima infections. Fecal oocyst shedding decreased significantly (p Aloe vera as compared to the unsupplemented group. Furthermore, the Aloe vera-supplemented group showed significantly fewer intestinal lesions (p Aloe vera could be used an alternative treatment for controlling avian coccidiosis.

  16. The combined effects of Aloe vera gel and silver nanoparticles on wound healing in rats

    Directory of Open Access Journals (Sweden)

    Y. Yousefpoor

    2016-01-01

    Full Text Available Objective(s: This study was aimed at investigating the synergy effects of Aloe vera gel and silver nanoparticles on the healing rate of the cutting wounds. Materials and Methods: In order to determine the concentration of silver nanoparticles in Aloe vera gel, the MBC methods were applied on the most common bacteria infecting wounds, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa. The cutting wounds with Full-thickness skin were dorsally created on rats; then the rats were divided into 4 groups. The treatments groups included: mixture of Aloe vera gel and silver nanoparticles, Aloe vera gel alone and silver nanoparticles alone in addition to control groups. The treatment was carried out for 2 weeks and the size of the wound closures were measured by an image software analysis. Results:There was no significant difference (p

  17. Aloe vera: a valuable ingredient for the food, pharmaceutical and cosmetic industries--a review.

    Science.gov (United States)

    Eshun, Kojo; He, Qian

    2004-01-01

    Scientific investigations on Aloe vera have gained more attention over the last several decades due to its reputable medicinal properties. Some publications have appeared in reputable Scientific Journals that have made appreciable contributions to the discovery of the functions and utilizations of Aloe--"nature's gift." Chemical analysis reveals that Aloe vera contains various carbohydrate polymers, notably glucomannans, along with a range of other organic and inorganic components. Although many physiological properties of Aloe vera have been described, it still remains uncertain as to which of the component(s) is responsible for these physiological properties. Further research needs to be done to unravel the myth surrounding the biological activities and the functional properties of A. vera. Appropriate processing techniques should be employed during the stabilization of the gel in order to affect and extend its field of utilization.

  18. GROSS TOXICITIES AND HEPATOPROTECTIVE EFFECT OF ALOE VERA (L BURM.F

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    Nuzhat Sultana

    2012-10-01

    Full Text Available Traditional herbal drugs have a great demand in under developed countries due to their efficacy, low cost and lesser adverse effects, and they are considered to be “natural. Aloe vera at the dose of 500 mg body weight per oral was studied for the gross toxicities and hepatoprotective effect and observed the level of liver biochemical parameters in rabbits. Aloe vera showed highly significant (p<0.001 hepatoprotective effect by lowering the serum levels of serum glutamic oxaloacetate transaminase (SGOT, serum glutamic pyruvates transaminase (SGPT and direct bilirubin. The overall experimental results suggests that Aloe vera protects the liver from oxidative stress and inhibits the excessive free radicals accumulation and possessing many hepatoprotective phytoconstituents which are biologically active such as flavonoids, alkaloids, they may be responsible for the significant hepatoprotective activity and the results justify the use of Aloe vera as a hepatoprotective agent.

  19. Evolutionary history and leaf succulence as explanations for medicinal use in aloes and the global popularity of Aloe vera

    DEFF Research Database (Denmark)

    Grace, Olwen Megan; Buerki, Sven; Symonds, Matthew RE

    2015-01-01

    the rich traditions of medicinal use and leaf succulence in aloes. Results: The phylogenetic hypothesis clarifies the origins of Aloe vera to the Arabian Peninsula at the northernmost limits of the range for aloes. The genus Aloe originated in southern Africa ~16 million years ago and underwent two major...

  20. Tyrosinase inhibitory components from Aloe vera and their antiviral activity.

    Science.gov (United States)

    Kim, Jang Hoon; Yoon, Ju-Yeon; Yang, Seo Young; Choi, Seung-Kook; Kwon, Sun Jung; Cho, In Sook; Jeong, Min Hee; Ho Kim, Young; Choi, Gug Seoun

    2017-12-01

    A new compound, 9-dihydroxyl-2'-O-(Z)-cinnamoyl-7-methoxy-aloesin (1), and eight known compounds (2-9) were isolated from Aloe vera. Their structures were elucidated using 1D/2D nuclear magnetic resonance and mass spectra. Compound 9 exhibited reversible competitive inhibitory activity against the enzyme tyrosinase, with an IC50 value of 9.8 ± 0.9 µM. A molecular simulation revealed that compound 9 interacts via hydrogen bonding with residues His244, Thr261, and Val283 of tyrosinase. Additionally, compounds 3 and 7 were shown by half-leaf assays to exhibit inhibitory activity towards Pepper mild mottle virus.

  1. SYNTHESIS AND CHARACTERIZATION OF BIODEGRDABLE PLASTIC FROM CASAVA STARCH AND ALOE VERA EXTRACT WITH GLYCEROL PLASTICIZER

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    Mery Apriyani

    2016-05-01

    Full Text Available Synthesis and characterizations of Biodegradable Plastic made of Cassava Waste Starch, glycerol, acetic acid and Aloe vera extract has done. The aims of this research are to study the influence of addition of aloe vera extract in plastics mechanics properties, water vapor transmission rate and biodegradation. There are five main steps in this research, extraction of aloe vera, cassava starch preparation from cassava waste, preparations, characterization and biodegradability study of biodegradable plastic. The addition variations of aloe vera extract that used in this research are 0.01; 0.03; 0.05; 0.07 and 0.14 grams. Results showed that the addition of aloe Vera tends to increased biodegrable plastic thickness to 0.01 mm and elongation to 32.07%. However, biodegradable plastic tensile strength tends to decreased to 23.95 Mpa. Optimum tensile strength is 3.90 Mpa and elongation is 34.43%. Optimum water vapor transmission rate is 2.40 g/m2hours. Biodegradation study of biodegradable plastic showed that addition of aloe vera extract doesn’t significantly influence in plastic degradations.

  2. Hepatotherapeutic effect of Aloe vera in alcohol-induced hepatic damage.

    Science.gov (United States)

    Saka, W A; Akhigbe, R E; Ishola, O S; Ashamu, E A; Olayemi, O T; Adeleke, G E

    2011-07-15

    There is a lack of reliable hepatotherapeutic drugs in modern medicine in the management of alcohol/drug-induced liver damage. Aloe vera extract has been used in folklore medicine for its medicinal values. This study evaluates the hepatotherapeutic activity of aqueous extract of Aloe vera gel in rats. Sprague-Dawley rats were divided into three groups; the negative control, positive control and the extract-treated groups. The negative control received only distilled water daily. The positive control received alcohol, while the extract-treated group received aqueous extract of Aloe vera and alcohol. Hepatotoxicity was induced in the positive control and extract-treated rats with alcohol. The hepatotherapeutic effect was evaluated by performing an assay of the serum total bilirubin, alkaline phosphatase, aspartate and alanine transaminases and liver histopathology. Alanine transaminase activities were comparable in all groups. Alcohol treatment alone significantly (p Aloe vera extract. Histopathological examination revealed that alcohol induced hepatic damage. Aloe vera treatment maintained hepatic architecture similar to that seen in the control. This study shows that aqueous extract of Aloe vera gel is hepatotherapeutic and thus lends credence to the use of the plant in folklore medicine in the management of alcohol-induced hepatic dysfunction.

  3. [Bacteriostatic and/or bactericidal extract of Aloe vera gel on cultures of Listeria monocytogenes].

    Science.gov (United States)

    Ramírez Mérida, Luis Guillermo; Morón de Salim, Alba; Catinella, Rosangela; Castillo, Luis

    2012-03-01

    Listeria monocytogenes is a bacteria responsible for food borne diseases (FBD). The effect of Aloe vera gel extract as a possible bacteriostatic and/or bactericidal against Listeria monocytogenes, was checked by determined the minimum inhibitory concentration (MIC), the time of minimum inhibition (TMI) and minimum bactericidal concentration (MBC) solutions extract of Aloe vera gel in different concentrations on cultures of Listeria monocytogenes ATCC 7635. We applied the agar diffusion method, using solutions of extract of Aloe vera gel at concentrations of 0 to 100% for the MIC. The TMI was determined by growth curves in trypticase soy broth with an initial inoculum of Listeria monocytogenes ATCC 7635 of 108 CFU/mL in each solution. It was determined that the MIC was 10% extract of Aloe vera gel and TMI was 5 hours at concentrations of 10%, 20% and 30% of Aloe vera, while concentrations of 50, 80, 90 and 100%, the time was 8 hours. It was found that indeed the Aloe vera gel is bacteriostatic power on Listeria monocytogenes (p < 0.001), but yet, no bactericidal effect was obtained in our study.

  4. Phytochemical standardization of Aloe vera extract by HPTLC techniques

    Institute of Scientific and Technical Information of China (English)

    Dinesh K Patel; Kanika Patel; SP Dhanabal

    2012-01-01

    Objective: To examine the phytochemical parameters of Aloe vera (A. vera) L. which can be used as a tool for its standardization. Methods: The phytochemical analysis, solubility test, heavy metal analysis, antimicrobial study and quantitative analysis of gallic acid and berberine by HPTLC method were included in present study. Results: Phytochemical analysis revealed the presence of alkaloid, carbohydrate, tannin, steroid, triterpenoid and glycoside. Total flavonoid and phenol content was found to be 1.9% and 13.11%. Concentartion of lead, arsenic, mercury and cadmium was found to be under the limit. Total bacterial count, yeast and moulds contents were found to be under the limit whereas Escherichia coli (E. coli) and salmonella was found to be absent in the extract. Quantitative analysis through HPTLC revealed the presence of 2.74%and 0.543% w/w of berberine and gallic acid. Conclusions: The results indicate that the plant extract are rich in berberine and gallic acid implying their importance to human health. This investigation could be used as source of standard parameters which can play an important role in its standardization.

  5. Revisión de la aloe vera (Barbadensis Miller) en la dermatología actual Revision Of Aloe Vera (Barbadensis Miller) In Actual Dermatology

    OpenAIRE

    2009-01-01

    El Aloe vera es una planta de uso popular en el tratamiento de algunas enfermedades de la piel y de aplicación frecuente en la cosmetología. Forma parte de diversas cremas o geles, siendo usado como antiinflamatorio y reconstituyente del tejido epitelial. Aunque se conocen varios mecanismos de acción e indicaciones clínicas, se debe realizar una revisión de las mismas para su uso, avalados por ensayos controlados para determinar su eficacia real.The Aloe vera is a popular plant used in the tr...

  6. Preparation of Polyamide Nanocapsules of Aloe vera L. Delivery with In Vivo Studies

    OpenAIRE

    Esmaeili, Akbar; Ebrahimzadeh, Maryam

    2014-01-01

    Aloe vera is the oldest medicinal plant ever known and the most applied medicinal plant worldwide. The purpose of this study was to prepare polyamide nanocapsules containing A. vera L. by an emulsion diffusion technique with in vivo studies. Diethyletriamine (DETA) was used as the encapsulating polymer with acetone ethyl acetate and dimethyl sulfoxide (DMSO) as the organic solvents and Tween and gelatin in water as the stabilizers. Sebacoyl chloride (SC) monomer, A. vera L. extract, and olive...

  7. Effects of drying temperature and ethanol concentration on bipolar switching characteristics of natural Aloe vera-based memory devices.

    Science.gov (United States)

    Lim, Zhe Xi; Cheong, Kuan Yew

    2015-10-28

    Extracted, formulated, and processed natural Aloe vera has been used as an active layer for memory applications. The functional memory device is realized by a bottom-up structure of ITO/Aloe vera/Al in which the Aloe vera is spin-coated after mixing with different concentrations of ethanol (0-80 wt%) and subsequently dried at different temperatures (50-120 °C). From the current density-voltage measurements, the device can exhibit a reproducible bipolar switching characteristic with pure Aloe vera dried at 50 °C. It is proposed that charges are transported across the Aloe vera layer via space-charge-limited conduction (SCLC), and clusters of interstitial space formed by the functional groups of acemannans and de-esterified pectins in the dried Aloe vera contribute to the memory effect. The formation of charge traps in the Aloe vera layer is dependent on the drying temperature. The drying temperature of a memory-switching Aloe vera layer can be extended to 120 °C with the addition of appropriate amounts of ethanol. The concept of using natural Aloe vera as an active material for memory applications has been demonstrated, and the read memory window, ON/OFF ratio, and retention time are approximately 5.0 V, 10(3), and >10(4) s, respectively.

  8. IDENTIFICATION OF PHYTO-COMPONENTS AND ITS BIOLOGICAL ACTIVITIES OF ALOE VERA THROUGH THE GAS CHROMATOGRAPHY-MASS SPECTROMETRY

    Directory of Open Access Journals (Sweden)

    P.T.V Lakshmi

    2011-05-01

    Full Text Available In this study, the bioactive components of Aloe vera leaves have been evaluated using GC/MS. The chemical compositions of the n-hexane extract of Aloe vera were investigated using Perkin-Elmer Gas Chromatography–Mass Spectrometry, while the mass spectra of the compounds found in the extract was matched with the National Institute of Standards and Technology (NIST library. GC/MS analysis of n-hexane extract of Aloe vera revealed the existence of twenty six bioactive compounds. The results of this study offer a platform of using Aloe vera as herbal drug for cancer studies.

  9. Stimulation of osteoblast activity by induction of Aloe vera and xenograft combination

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2011-12-01

    Full Text Available Background: Tooth extraction is generally followed by alveolar ridge resorption that later can cause flat ridge. Aloe vera have biogenic stimulator and hormone activities for wound healing. Purpose: This study was aimed to know osteoblast activities in alveolar bone after induction of Aloe vera and XCB combination. Methods: Fifty four of Cavia cabaya were divided into three main groups. Group I was control group. Group II was filled with xenograft concelous bovine (XCB and group III was filled with the combination of Aloe vera gel and XCB. Then, each group was divided into three sub groups according to timing, they are 14, 30, and 60 days after tooth extraction and application. Histology and morphology examination were performed on the harvested specimens. Results: There were significant differences between the control group and the other groups filled with the combination of Aloe vera and XCB. Conclusion: In conclusion, the application of Aloe vera gel and xenograft combination decrease the number of osteoclast and increase the number of osteoblast in post tooth extraction alveolar bone structure indicating the new growth of alveolar bone.Latar belakang: Pencabutan gigi pada umumnya selalu diikuti resopsi tulang alveolar, sehingga bila terjadi dalam waktu yang lama ridge akan menjadi flat. Aloe vera adalah bahan stimulasi biogenik dan mempunyai aktivitas hormon untuk proses penyembuhan luka. Tujuan: Tujuan dari penelitian ini adalah untuk mengetahui aktivitas osteoblas pada tulang alveol dengan pemberian kombinasi Aloe vera gel dan xenograft concelous bovine (XCB. Metode: Lima puluh empat ekor Cavia cabaya, dibagi menjadi 3 kelompok besar, kelompok pertama adalah kelompok kontrol yaitu hanya dilakukan pencabutan saja tanpa perlakuan, kelompok ke-2 yaitu kelompok yang setelah dicabut diberi XCB saja dan kelompok ke-3 yaitu kelompok yang setelah pencabutan diberi kombinasi Aloe vera gel dengan XCB pada luka bekas pencabutan gigi. Kemudian masing

  10. Acute hepatitis induced by an Aloe vera preparation: A case report

    Institute of Scientific and Technical Information of China (English)

    Christian Rabe; Annemarie Musch; Peter Schirmacher; Wolfgang Kruis; Robert Hoffmann

    2005-01-01

    AIM: Aloe vera, plant extracts of Aloe barbadensis miller,is widely used in phytomedicine. The first case of acute hepatitis due to this compound was described.METHODS: Description of a clinical case.RESULTS: Hepatitis in a 57-year old female could be linked to the ingestion of Aloe barbadensis miller compounds. The patient's hepatitis resolved completely after discontinuing this medication.CONCLUSION: The case emphasizes the importance of considering phytopharmaceutical over-the-counter drugs as causative agents of hepatitis.

  11. Acute toxic hepatitis caused by an aloe vera preparation in a young patient: a case report with a literature review.

    Science.gov (United States)

    Lee, Jeonghun; Lee, Mi Sun; Nam, Kwan Woo

    2014-07-01

    Aloe is one of the leading products used in phytomedicine. Several cases of aloe-induced toxic hepatitis have been reported in recent years. However, its toxicology has not yet been systematically described in the literature. A 21-year-old female patient was admitted to our hospital with acute hepatitis after taking an aloe vera preparation for four weeks. Her history, clinical manifestation, laboratory findings, and histological findings all led to the diagnosis of aloe vera-induced toxic hepatitis. We report herein on a case of acute toxic hepatitis induced by aloe vera.

  12. Calendula

    Science.gov (United States)

    ... On Ltd) that contains mullein, garlic, calendula, and St. John’s wort to the ear for 3 days reduces ear ... calamus, California poppy, catnip, hops, Jamaican dogwood, kava, St. John's wort, skullcap, valerian, yerba mansa, and others.

  13. Composite wound dressings of pectin and gelatin with aloe vera and curcumin as bioactive agents.

    Science.gov (United States)

    Tummalapalli, Mythili; Berthet, Morgane; Verrier, Bernard; Deopura, B L; Alam, M S; Gupta, Bhuvanesh

    2016-01-01

    Aloe vera and curcumin loaded oxidized pectin-gelatin (OP-Gel) matrices were used as antimicrobial finishes on nonwoven cotton fabrics to produce composite wound care devices. The drug release characteristics of the biocomposite dressings indicated that curcumin is released through a biphasic mechanism - erosion of the polymeric matrix, followed by diffusion, while aloe vera is released upon leaching of the polymeric matrix. A 50/50 composition of aloe vera/curcumin was used to fabricate OP-Gel-Aloe Curcumin dressings. However, contrary to our expectations, OP-Gel-Aloe Curcumin dressings exhibited lesser antimicrobial activity compared to OP-Gel-Aloe and OP-Gel-Curcumin dressings. The cytocompatibility of the fabricated dressings was evaluated using NIH3T3 mouse fibroblast cells. OP-Gel-Aloe treated fibroblasts had the highest viability, with the matrices providing a substrate for good cell attachment and proliferation. On the other hand, OP-Gel-Curcumin and OP-Gel-Aloe Curcumin seemed to have induced apoptosis in NIH3T3 cells. In vivo wound healing analysis was carried out using an excisional splint wound model on C57BL/6J mice. OP-Gel-Aloe treated wounds exhibited very rapid healing with 80% of the wound healing in just 8 days. Furthermore, aloe vera exerted a strong anti-inflammatory effect and prominent scar prevention. Histological examination revealed that an ordered collagen formation and neovascularization could be observed along with migration of nuclei. Therefore, OP-Gel-Aloe biocomposite dressings are proposed as viable materials for effective wound management.

  14. Effect of dietary aloe vera on growth and lipid peroxidation indices in rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Golestan, Ghazale; Salati, Amir Parviz; Keyvanshokooh, Saeed; Zakeri, Mohammad; Moradian, Hossein

    2015-01-01

    Aloe vera has been used worldwide in pharmaceutical, food and cosmetic industries due to the plethora of biological activities of its constituents. This study was done to evaluate the effects of dietary aloe vera on growth and lipid peroxidation in rainbow trout (Oncorhynchus mykiss). A total number of 480 O. mykiss (mean weight 9.50 ± 0.85 g) were randomized into four experimental groups including one control and three experimental groups that aloe vera was incorporated in their diet at 0.5, 1.0 and 2.0 g kg(-1). Trial was done for eight weeks. Then biometry and blood sampling were done. Plasma malondialdehyde, ferric reducing ability of plasma and growth index were estimated at the end of study. The results showed that aloe vera extract did not affect growth indices. Malondialdehyde was increased in the experimental group compared to the control group but ferric reducing ability of plasma showed a decrease in experimental groups (p aloe vera have adverse effects on antioxidant defense system in O. mykiss.

  15. The Use of Aloe Vera Extract as a Novel Storage Media for the Avulsed Tooth

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    Samaneh Badakhsh

    2014-07-01

    Full Text Available Background: Tooth avulsion is one of the most severe dental traumas which most often occur in children. When immediate replantation is not possible, storage in a proper media may lead to a prolonged survival rate. Aloe Vera is a cactus like plant with green, tapered leaves that are filled with a transparent viscous gel. This medicinal plant has significant anti-inflammatory, antioxidant, antibacterial and antifungal effects. The purpose of this study was to assess the effectiveness of different concentrations of Aloe Vera extract compared to DMEM (cell culture medium and egg white. Methods: The periodontal ligament (PDL cells were cultured and certain number of cells were treated with Aloe Vera extract (in four different concentrations, egg white and culture media for 1, 3, 6, and 9 hours. Cell viability was determined by using the (3-[4, 5-dimethylthiazolyl-2]-2, 5-diphenyltetrazolium bromide assay. Moreover, One-way ANOVA and post hoc (LSD test were used for analyzing the study groups. Results: The results indicate that culture media and Aloe Vera extract (10, 30, and 50% concentration were statistically similar and significantly preserved more PDL cells compared to other experimental storage media. Conclusion: Aloe Vera 10, 30, and 50% may be recommended as a suitable storage media for avulsed teeth.

  16. Administration of dried Aloe vera gel powder reduced body fat mass in diet-induced obesity (DIO) rats.

    Science.gov (United States)

    Misawa, Eriko; Tanaka, Miyuki; Nabeshima, Kazumi; Nomaguchi, Kouji; Yamada, Muneo; Toida, Tomohiro; Iwatsuki, Keiji

    2012-01-01

    The aim of the present study was to investigate the anti-obesity effects of Aloe vera gel administration in male Sprague-Dawley (SD) rats with diet-induced obesity (DIO). SD rats at 7 wk of age were fed either a standard diet (10 kcal% fat) (StdD) or high-fat (60 kcal% fat) diet (HFD) during the experimental period. Four weeks after of HFD-feeding, DIO rats (11 wk of age) were orally administered with two doses of Aloe vera gel powder (20 and 200 mg/kg/d) for 90 d. Body weights (g) and body fat (%) of HFD fed rats were significantly higher than those of StdD-fed rats. Although a modest decrease of body weight (g) was observed with the administration of dried Aloe vera gel powder, both subcutaneous and visceral fat weight (g) and body fat (%) were reduced significantly in Aloe vera gel-treated rats. Serum lipid parameters elevated by HFD were also improved by the Aloe vera gel treatment. The oxygen consumption (VO(2)), an index of energy expenditure, was decreased in HFD-fed rats compared with that in StdD-fed rats. Administration of Aloe vera gel reversed the change in VO(2) in the HFD-fed rats. These results suggest that intake of Aloe vera gel reduced body fat accumulation, in part, by stimulation of energy expenditure. Aloe vera gel might be beneficial for the prevention and improvement of diet-induced obesity.

  17. Processed Aloe vera Gel Ameliorates Cyclophosphamide-Induced Immunotoxicity

    Directory of Open Access Journals (Sweden)

    Sun-A Im

    2014-10-01

    Full Text Available The effects of processed Aloe vera gel (PAG on cyclophosphamide (CP-induced immunotoxicity were examined in mice. Intraperitoneal injection of CP significantly reduced the total number of lymphocytes and erythrocytes in the blood. Oral administration of PAG quickly restored CP-induced lymphopenia and erythropenia in a dose-dependent manner. The reversal of CP-induced hematotoxicity by PAG was mediated by the functional preservation of Peyer’s patch cells. Peyer’s patch cells isolated from CP-treated mice, which were administered PAG, produced higher levels of T helper 1 cytokines and colony-stimulating factors (CSF in response to concanavalin A stimulation as compared with those isolated from CP-treated control mice. PAG-derived polysaccharides directly activated Peyer’s patch cells isolated from normal mice to produce cytokines including interleukin (IL-6, IL-12, interferon-γ, granulocyte-CSF, and granulocyte-macrophage-CSF. The cytokines produced by polysaccharide-stimulated Peyer’s patch cells had potent proliferation-inducing activity on mouse bone marrow cells. In addition, oral administration of PAG restored IgA secretion in the intestine after CP treatment. These results indicated that PAG could be an effective immunomodulator and that it could prevent CP-induced immunotoxic side effects.

  18. Strong enhancement of antioxidant activity of Aloe vera extracts by gamma irradiation

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Eun Mi; Bai, Hyoung Woo; Lee, Seung Sik; Hong, Sung Hyun [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of); Cho, Jae Young; Chung, Byung Yeoup [Chonbuk National University, Jeonju (Korea, Republic of)

    2011-10-15

    The World Health Organization (WHO) has estimated that approximately 80% of individuals rely on traditional medicines. Among over 400 Aloe species, Aloe vera was the most accepted species for various medical, cosmetic and neutraceutical purposes. Aloe vera (syn.: Aloe barbadensis Miller) was a perennial succulent plant belonging to the Aloeaceae family (subfamily of the Asphodelaceae). It has been reported that Aloe vera extracts were useful in the treatment of wound and burn healing, minor skin infections, sebaceous cyst, diabetes, and elevated blood lipids in humans. Recent studies have shown that treatment with either Aloe vera crude gel or its extracts, such as acemannan, {beta}-sitosterol, and others, resulted in faster healing of wounds by stimulating fibroblast proliferation, collagen deposition, angiogenesis, and production of growth factors. Ionizing radiation technology has been developed to improve our daily life such as cancer therapy and sterilizing tool due to its unique feature that could be penetrated biomaterials leading to alter their own physical properties. More recently, many studies have attempted to apply the radiation technology to enhance their biological activities. At present, however, very little was known about whether naturally-occurring phenolic compounds of ethanolic aloe gel extracts that were altered their biological activities by ionizing radiation to serve as antioxidant in the body to reduce ROS produced by the stresses. The purpose of the current study was to investigate the influence of gamma irradiation on antioxidant activity of Aloe vera extracts, and open insight new possibilities that gamma ray could be a powerful tool for improving its own biological activities

  19. The effects of crude aqueous and alcohol extracts of Aloe vera on growth and abdominal viscera of suckling rats.

    Science.gov (United States)

    Beya, Wabeya; Davidson, Bruce; Erlwanger, Kennedy H

    2012-01-01

    The gastrointestinal tract of neonates is sensitive to dietary manipulations. When nursing mothers use Aloe vera, their babies are at risk of indirect exposure to Aloe vera via breast feeding or directly as health supplements. The effects of orally administered extracts of Aloe vera in unweaned rats were investigated. Six day old Sprague-Dawley rats were gavaged with aqueous or alcohol extracts of Aloe vera (low dose 50mg. kg⁻¹ or high dose 500mg. kg⁻¹) daily for eight days. All data were expressed as mean ± SD and analyzed by one way ANOVA. Pups receiving high doses of either extract had a significantly higher body mass gain than the group receiving lower dose (p Aloe vera extracts resulted in growth promotion, enhanced hepatic storage of metabolic substrates, increased ALP possibly in relation to bone growth and caused hypertrophy of the caecum of neonatal rats. These effects need to be explored further to enhance animal production and health.

  20. Aloe vera is non-toxic to cells: A microculture tetrazolium technique colorimetric assay study

    Directory of Open Access Journals (Sweden)

    Devi Gopakumar

    2014-01-01

    Full Text Available Introduction: Aloe vera (Av, a succulent of Liliaceae family is now a widely used medicinal plant. Its′ application covers a wide spectrum of human diseases, including oral mucosa, gastric mucosa and skin. Aloe vera preparations in the form of gel, mouth washes and cream are applied topically for many oral diseases. The applications include oral lichen planus, candidiasis, oral submucous fibrosis, geographic tongue, etc. Aims and Objectives: To evaluate the cytotoxicity of Av on human fibroblasts. Materials and Methods: Aloe vera preparation (70% was applied on the fibroblast cell lineage and the cell viability was evaluated by microculture tetrazolium technique (MTT colorimetric assay. Results: The cell viability at different concentrations was measured. The cells have maintained their viability at different concentrations used in the study. Conclusion: Our study shows the cell viability at different sample concentrations of Av. This could open up wide clinical applications of Av for reactive, inflammatory and potentially malignant oral and other mucocutaneous diseases.

  1. RBF neural network prediction on weak electrical signals in Aloe vera var. chinensis

    Science.gov (United States)

    Wang, Lanzhou; Zhao, Jiayin; Wang, Miao

    2008-10-01

    A Gaussian radial base function (RBF) neural network forecast on signals in the Aloe vera var. chinensis by the wavelet soft-threshold denoised as the time series and using the delayed input window chosen at 50, is set up to forecast backward. There was the maximum amplitude at 310.45μV, minimum -75.15μV, average value -2.69μV and Aloe vera var. chinensis respectively. The electrical signal in Aloe vera var. chinensis is a sort of weak, unstable and low frequency signals. A result showed that it is feasible to forecast plant electrical signals for the timing by the RBF. The forecast data can be used as the preferences for the intelligent autocontrol system based on the adaptive characteristic of plants to achieve the energy saving on the agricultural production in the plastic lookum or greenhouse.

  2. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study).

    Science.gov (United States)

    Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

    2013-01-01

    Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel effect on Staphylococcus aureus and Streptococcus mutans was evaluated. Disk diffusion test revealed that propolis ethanolic extracts (the alcohol concentration of 15 and 40%) and Aloe vera gel have antibacterial activities but aqueous extract of propolis did not show any effect in this test. The MICs for propolis ethanolic extracts, Aloe vera gel and aqueous extract of propolis (0% alcohol) were 313 µg/ml, 750 µg/ml, 2250 µg/ml, and ≥ 500 µg/ml respectively, much higher than the Chlorhexidine one. In direct contact test, contrary to Aloe vera, all three propolis extracts showed antibacterial effects on E. faecalis. The Aloe vera gel also showed significant antibacterial effect on S.aureus and S.mutans. The hydroalcoholic extracts of propolis and Aloe vera gel had antibacterial effects on E. faecalis, however, propolis is more potent than Aloe vera. The antibacterial effect of Aloe vera on S. aureus and S. mutans is low (MIC ≥ 2250 µg/ml). Appropriate concentrations of alcoholic extracts of propolis and some fractions of Aloe vera gel might be good choices for disinfecting the root canal in endodontic treatments.

  3. Phytomodulatory potentials of Aloe vera against Salmonella OmpR-mediated inflammation.

    Science.gov (United States)

    Rishi, Praveen; Rampuria, Anjali; Tewari, Rupinder; Koul, Ashwani

    2008-08-01

    Mediators released during inflammatory response play an essential role in eliminating microbes or microbial products. However, the uncontrolled release of cytotoxic substances characterized by extensive inflammation may adversely affect normal tissues. Under such conditions it is important to manage the hyperinflammation in order to change the clinical manifestations of the disease. Accordingly, the present study was designed to evaluate the modulation of Salmonella OmpR mediated inflammation by Aloe vera, a plant known to contain antiinflammatory ingredients. It was observed that outer-membrane proteins (OMPs) extracted from the wild type strain of S. typhimurium caused inflammation of greater magnitude compared with the OMPs extracted from its mutant construct as evident from the oedema test as well as the hyperalgesic (flicking) response of the animals under experimental conditions. However, Aloe vera applied topically, administered intraperitoneally or in combination modulated the inflammatory response. The maximum effect was observed with the combined formulation indicating modulation at local as well as systemic levels. The results reveal that this modulation could be due to the potential of Aloe vera to decrease peroxidative damage via a decrease in the levels of monokines (TNF-alpha, IL-1 and IL-6) and an increase in the level of superoxide dismutase (SOD). Moreover, the presence of SOD in Aloe vera itself might be responsible for enhancing its levels in the macrophages. On the other hand, no significant change in the catalase activity was observed by Aloe vera treatment. The use of Aloe vera, therefore, seems to have a promising role in the modulation of Salmonella OmpR mediated inflammation.

  4. Therapeutic approach by Aloe vera in experimental model of multiple sclerosis.

    Science.gov (United States)

    Mirshafiey, A; Aghily, B; Namaki, S; Razavi, A; Ghazavi, A; Ekhtiari, P; Mosayebi, G

    2010-09-01

    Multiple sclerosis (MS) is an autoimmune disease of the central nervous system (CNS) that leads to an inflammatory demyelination, axonal damage, and progressive neurologic disability that affects approximately 2.5 million people worldwide. The aim of the present research was to test the therapeutic effect of Aloe vera in experimental model of MS. All experiments were conducted on C57BL/6 male mice aged 6-8 weeks. To induce the experimental autoimmune encephalomyelitis (EAE), 250 microg of the myelin oligodendrocyte glycoprotein 35-55 peptide emulsified in complete freund's adjuvant was injected subcutaneously on day 0 over two flank areas. In addition, 200 ng of pertussis toxin in 100 microL phosphate buffered saline was injected intraperitoneally on days 0 and 2. The therapeutic protocol was carried out intragastrically using 120 mg/kg/day Aloe vera from 7 days before to 21 days after EAE induction. The mice were killed 21 days after EAE induction. The brains of mice were removed for histological analysis and their isolated splenocytes were cultured. The results indicated that treatment with Aloe vera caused a significant reduction in severity of the disease in experimental model of MS. Histological analysis showed 3 +/- 2 plaques in Aloe vera-treated mice compared with 5 +/- 1 plaques in control group. The density of mononuclear infiltration in the CNS of Aloe vera-treated mice (500 +/- 200) was significantly less in comparison to 700 +/- 185 cells in control group. Moreover, the serum level of nitric oxide in treatment group was significantly less than control animals. The level of interferon-gamma in cell culture supernatant of treated mice splenocytes was lower than control group, whereas decrease in serum level of interleukin-10 in treatment group was not significant in comparison with control mice. These data indicate that Aloe vera therapy can attenuate the disease progression in experimental model of MS.

  5. Evaluation of anti-inflammatory effect of aqueous extract of Aloe vera in Albino rats

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    Devarsi Choudhury

    2016-12-01

    Conclusions: In Adjuvant arthritis method for chronic inflammation, paw edema of the injected and the non-injected were measured on the 5th day and the 21st day respectively. Arthritic index was measured on the 21st day. These results were highly significant for AEAV and Indomethacin in comparison with the control. The results for the arthritic index were highly significant for both Aloe vera and Indomethacin. It is easily illustrated that the herbal plant Aloe vera at the dose of 500mg/kg demonstrated significant anti-inflammatory effect against all the experimental methods on inflammation. [Int J Basic Clin Pharmacol 2016; 5(6.000: 2488-2495

  6. Aloe vera COMO SUSTRATO PARA EL CRECIMIENTO DE Lactobacillus plantarum y L. casei

    OpenAIRE

    B. A González; R. Domínguez-Espinosa; B. R. Alcocer

    2008-01-01

    En este estudió se determinó el efecto del uso de jugo de Aloe vera (sábila) como sustrato principal de fermentación para obtener cultivos de alta concentración de células viables de dos bacterias con actividad probiótica: Lactobacillus plantarum (NCIMB 11718) y Lactobacillus casei (NRRL -1445). Se determinó la velocidad específica de crecimiento (u) de cada microorganismo en medios con diferentes concentraciones de Aloe vera comparándolos con aquellos obtenidos en cultivos crecidos en medio ...

  7. Pharmacological attribute of Aloe vera: Revalidation through experimental and clinical studies.

    Science.gov (United States)

    Gupta, Vinay K; Malhotra, Seema

    2012-04-01

    Aloe vera has long been used as a traditional medicine for inducing wound healing. It is a natural product that now a days is used in cosmetic industry. Benefits associated with Aloe vera have been attributed to the polysaccharides contained in the gel of the leaves though there are various indications for its use. Biological activities include promotion of wound healing, antifungal activity, anti-inflammatory, anticancer and immunomodulatory. Gingival fibroblasts play an important role in oral wound healing. Double blind-controlled trials are needed to determine its real efficacy in oral health.

  8. Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens

    OpenAIRE

    Jagmohan Singh; K.M. Koley; Khushboo Chandrakar; Nileshkumar S. Pagrut

    2013-01-01

    Aim: To evaluate the effects of Aloe vera on dressing percentage and hemato-biochemical parameters of broiler chickens.Materials and Methods: A total of 90 chicks were used in this study. They were randomly allocated into 3 treatment groups.Fresh Aloe vera leaf juice (ALJ) was prepared and administered to the test group T3 at the rate of 20 g/Lin drinking water daily.This study was carried out for 42 days. Dressing percentage and hemato-biochemical parameters were recorded at the end ofexperi...

  9. Effects of highly purified anthraquinoid compounds from Aloe vera on sensitive and multidrug resistant leukemia cells.

    Science.gov (United States)

    Grimaudo, S; Tolomeo, M; Gancitano, R; Dalessandro, N; Aiello, E

    1997-01-01

    Folk medicine has attributed antitumor properties to preparations from Aloe vera. We have studied the effects of five purified compounds from the plant on human K562 leukemia and on its multidrug resistant (MDR) variant, K562/R. The glycosides aloin A and B, aloesin and aloeresin were devoid of antitumor activity up to 200 mu M concentrations. Only the aglycone aloe emodin produced reproducible antitumor effects, which, interestingly, were more pronounced in the MDR, P-glycoprotein overexpressing, cell line. Its IC50 was in fact 29 mu M in K562 and 10.5 mu M in K562/R. Aloe emodine caused mainly cytostasis and accumulation of the cells in the S and G(2)-M phases of the cell cycle during the first 48 h of treatment. Thereafter, massive cell death ensued. Research on the antitumor activity of compounds extracted from Aloe vera probably deserves continuation.

  10. Evaluation of in vitro and in vivo antioxidant potential of polysaccharides from Aloe vera (Aloe barbadensis Miller) gel.

    Science.gov (United States)

    Kaithwas, Gaurav; Singh, Prashant; Bhatia, Daksh

    2014-04-01

    In the present study, the antioxidant activity of the polysaccharides from aloe vera (Aloe barbadensis Miller) gel was evaluated, in vitro by five established methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH(-)) radical scavenging, nitric oxide (NO) scavenging, hydrogen peroxide scavenging, superoxide radical (O(-2)) scavenging and reducing power assay, and in vivo against doxorubicin (DOX)-induced myocardial oxidative stress (OS) in albino wistar rats. The polysaccharides exhibited significant inhibitory activity against DPPH(-), superoxide, NO and hydrogen peroxide scavenging assay with significant reducing activity at all concentrations used. DOX-induced (7.5 mg/kg, intravenously) cardiotoxicity manifested biochemically by a significant decrease in blood and tissue glutathione (GSH) along with elevated levels of serum lactate dehydrogenase and creatine phosphokinase. In addition, cardiotoxicity was further confirmed by the significant increase in lipid peroxidation expressed as thiobarbituric acid reactive substances (TBARS), catalase (CAT) and superoxide dismutase (SOD). Administration of aloe vera polysaccharides for 14 days produced a marked protection against cardiotoxicity induced by DOX evidenced by significant reductions in serum lactate dehydrogenase, serum creatine phosphokinase, cardiac TBARS, CAT and SOD along with increased levels of blood and tissue GSH in a dose-dependent manner. The present investigation is the first to establish the antioxidant potency of the polysaccharides from aloe vera against DOX-induced myocardial OS.

  11. Effect of pomegranate and aloe vera extract on streptococcus mutans: An in vitro study

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    Priya Subramaniam

    2012-01-01

    Full Text Available Introduction: The aim of the present in vitro study was to evaluate and compare the antibacterial effect of pomegranate and aloe vera extracts on Streptococcus mutans. Materials and Methods: Hydroalcoholic extracts of pulp from both Punica granatum (pomegranate and Aloe barbadensis miller (aloe vera were prepared to concentrations of 5, 25, 50 and 100%. Pure sorbitol powder dissolved in distilled water was taken as the negative control. Streptococcus mutans (S mutans was isolated from saliva by inoculation on to Mitus Salivarius Bacitracin (MSB agar, which was then streaked onto agar plates containing Brain Heart Infusion. In each petridish, wells were prepared and using a sterile micropipette, 125μl of the specific concentration of the extract (pomegranate/ aloe vera/ sorbitol was deposited in each well. This was done in triplicate for each concentration of the extracts. The effect of different concentrations of the extracts on S mutans was observed and the data was subjected to statistical analysis. Results: Pomegranate extract showed significantly higher inhibitory effect on S mutans at all concentrations (P≤0.05. On comparison of all three extracts at different concentrations, a significant difference (P≤0.05 was observed only at 50 and 100% concentrations. The inhibitory effect of pomegranate extract was significantly different when compared to aloe vera and sorbitol extracts. (P≤0.01. Discussion: Pomegranate extract has a significant antibacterial effect on S mutans at all concentrations.

  12. Mechanism of Aloe Vera extract protection against UVA: shelter of lysosomal membrane avoids photodamage.

    Science.gov (United States)

    Rodrigues, Daniela; Viotto, Ana Cláudia; Checchia, Robert; Gomide, Andreza; Severino, Divinomar; Itri, Rosangela; Baptista, Maurício S; Martins, Waleska Kerllen

    2016-03-01

    The premature aging (photoaging) of skin characterized by wrinkles, a leathery texture and mottled pigmentation is a well-documented consequence of exposure to sunlight. UVA is an important risk factor for human cancer also associated with induction of inflammation, immunosuppression, photoaging and melanogenesis. Although herbal compounds are commonly used as photoprotectants against the harmful effects of UVA, the mechanisms involved in the photodamage are not precisely known. In this study, we investigated the effects of Aloe Vera (Aloe barbadensis mil) on the protection against UVA-modulated cell killing of HaCaT keratinocytes. Aloe Vera exhibited the remarkable ability of reducing both in vitro and in vivo photodamage, even though it does not have anti-radical properties. Interestingly, the protection conferred by Aloe Vera was associated with the maintenance of membrane integrity in both mimetic membranes and intracellular organelles. The increased lysosomal stability led to a decrease in lipofuscinogenesis and cell death. This study explains why Aloe Vera extracts offer protection against photodamage at a cellular level in both the UV and visible spectra, leading to its beneficial use as a supplement in protective dermatological formulations.

  13. 芦荟药理研究进展%Aloe Vera Pharmacological Research

    Institute of Scientific and Technical Information of China (English)

    刘学华; 赵红影; 严令耕; 李瑞; 潘苗苗

    2011-01-01

    分别从芦荟的生物活性与有效成分研究、外用功能的药理研究、作用机制研究、毒副作用和安全性研究5个方面进行了综述,并对芦荟外用功能研究进行了评述.芦荟具有很大的药用价值,尤其是外用治疗应用广泛,近年来芦荟产品的开发研究倍受青睐,应用范围日趋扩大,成为全世界关注的药食多用植物.%Aloe vera is a kind of Aloe ( L.) perennial evergreen. Aloe vera has big medicinal value.Especially topical therapy is widely used, more the favour of people. In recent years, our product of the pharmacological studies, made gratifying achievements. For more in-depth study of the use function,this paper aloe vera respectively from the biological activity of effective ingredients and pharmacological research, external function, mechanism, toxicity and safety of five aspects were summarized, and the function of aloe vera external research are reviewed.

  14. Revisión de la aloe vera (Barbadensis Miller en la dermatología actual Revision Of Aloe Vera (Barbadensis Miller In Actual Dermatology

    Directory of Open Access Journals (Sweden)

    GM Ferraro

    2009-12-01

    Full Text Available El Aloe vera es una planta de uso popular en el tratamiento de algunas enfermedades de la piel y de aplicación frecuente en la cosmetología. Forma parte de diversas cremas o geles, siendo usado como antiinflamatorio y reconstituyente del tejido epitelial. Aunque se conocen varios mecanismos de acción e indicaciones clínicas, se debe realizar una revisión de las mismas para su uso, avalados por ensayos controlados para determinar su eficacia real.The Aloe vera is a popular plant used in the treatment of certain skin diseases that has a frequent application in cosmetology. It forms a part of diverse creams or gels, being used as anti-inflammatory and restorative of epithelial tissue. Although there are several mechanisms of action and clinical indications, a review of its use should be done supported by controlled trials to determine its effectiveness.

  15. Influence of Aloe vera on water absorption and enzymatic in vitro degradation of alginate hydrogel films.

    Science.gov (United States)

    Pereira, Rúben F; Carvalho, Anabela; Gil, M H; Mendes, Ausenda; Bártolo, Paulo J

    2013-10-15

    This study investigates the influence of Aloe vera on water absorption and the in vitro degradation rate of Aloe vera-Ca-alginate hydrogel films, for wound healing and drug delivery applications. The influence of A. vera content (5%, 15% and 25%, v/v) on water absorption was evaluated by the incubation of the films into a 0.1 M HCl solution (pH 1.0), acetate buffer (pH 5.5) and simulated body fluid solution (pH 7.4) during 24h. Results show that the water absorption is significantly higher for films containing high A. vera contents (15% and 25%), while no significant differences are observed between the alginate neat film and the film with 5% of A. vera. The in vitro enzymatic degradation tests indicate that an increase in the A. vera content significantly enhances the degradation rate of the films. Control films, incubated in a simulated body fluid solution without enzymes, are resistant to the hydrolytic degradation, exhibiting reduced weight loss and maintaining its structural integrity. Results also show that the water absorption and the in vitro degradation rate of the films can be tailored by changing the A. vera content.

  16. Effect of Aloe vera on some indicators of cell damage after a period of aerobic exercise in male athletes

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    Barati Amir-Hosein

    2015-07-01

    Full Text Available Introduction: Aloe vera is a medicinal plant with antioxidant activity. The purpose of this study was to determine the effect of Aloe vera on some indicators of cell damage after a period of aerobic exercise in male athletes of 15-18 years old. Methods: In this clinical trial study, 20 male students with average weight of 64.85 ± 51 and height of 172.05 ± 6.4 were randomly assigned to 2 groups of Aloe vera supplement (n = 10 and placebo (n = 10. Then aerobic training was conducted for 4 weeks. The supplemented group took 3 capsules, each capsule contains 2 g of dried Aloe vera and placebo group took 3 capsules/day containing dextran after every meal. To determine the index of cell injury markers creatine kinase (CK, lactate dehydrogenase (LDH and reactive protein C (CRP were determined and blood samples were collected 24 hours before and after each test. The data were analyzed using ANOVA and independent t test. Significance level was considered as P ≤ .05. Results: The use of Aloe vera during aerobic exercise significantly reduced LDH (P = .006 in the supplement group (15% reduction compared to placebo group and CRP with 11% reduction in the supplement group compared to placebo (P = .008. There was no significant reduction in CK. Conclusion: The findings of this study showed that Aloe vera reduces cell damage and inflammation indicators. This result may reflect the role of Aloe vera as anti-inflammatory and antioxidant agent.

  17. Comparative evaluation of natural antioxidants spirulina and aloe vera for the treatment of oral submucous fibrosis

    Science.gov (United States)

    Patil, Santosh; Al-Zarea, Bader Kureyem; Maheshwari, Sneha; Sahu, Rohit

    2015-01-01

    Aim Oral submucous fibrosis (OSMF) is a high risk premalignant condition predominantly seen in the Indian subcontinent. The aim of the present study was to compare the efficacy of spirulina and aloe vera in the management of OSMF. Material and methods 42 subjects with clinico-pathologically diagnosed OSMF were included in the study and divided equally in 2 groups, Group A (spirulina group) and Group B (aloe vera group). Group A was administered 500 mg spirulina in 2 divided doses for 3 months and Group B was given 5 mg aloe vera gel to be applied topically thrice daily for 3 months. Evaluation for different clinical parameters was done at regular intervals and data was analyzed using the Chi-square test. P-value <0.05 was considered to be statistically significant. Results The patients in Group A showed significant clinical improvement in mouth opening and ulcers/erosions/vesicles (p < 0.05). However, there was no significant improvement in burning sensation (p = 0.06) and pain associated with the lesion (p = 0.04) among the 2 groups. Conclusion Both the drugs showed improvement in the condition; however spirulina can bring about significant clinical improvements in the symptoms like mouth opening and ulcers/erosion/vesicles. Thus, spirulina appears to be more promising when compared to aloe vera for the treatment of OSMF. PMID:25853042

  18. Disinfection of dental unit water line using aloe vera: in vitro study.

    Science.gov (United States)

    Pareek, Sonia; Nagaraj, Anup; Sharma, Prateek; Atri, Mansi; Walia, Satinder; Naidu, Shravani; Yousuf, Asif

    2013-01-01

    Context. Dental unit waterlines may be heavily contaminated with microorganisms and are a potential source of infection for both practicing staff and immunocompromised patients particularly. Contamination of dental unit water lines could be inhibited with the use of disinfectants. The present study investigates the effect of aloe-vera-based disinfectant in reducing the microbial growth in dental unit water lines (DUWLs). Aims. To compare the efficacy of aloe vera, hydrogen peroxide (H2O2), and 5% sodium hypochlorite (NaOCl) in controlling microbial contamination of DUWLs. Materials and Methods. After obtaining baseline water samples, the dental unit waterlines were treated with aloe vera, 10% hydrogen peroxide, and 5% sodium hypochlorite. Each of the three disinfectants was used in increasing concentrations and their inhibiting effect was compared. Water samples were analyzed for microbiological quality by the total viable count (TVC) method. Statistical Analysis Used. SPSS 16. Results. There was significant reduction in mean CFU/ml when treated with disinfectants each for a period of one week. Aloe-vera solution was found to be the most effective in reducing the microbial colonies. Conclusions. Improving the water quality from dental unit water lines is of considerable importance; chemical-based disinfectants can be replaced with herbal disinfectants for treating microbial contamination in dental unit waterlines.

  19. Disinfection of Dental Unit Water Line Using Aloe Vera: In Vitro Study

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    Sonia Pareek

    2013-01-01

    Full Text Available Context. Dental unit waterlines may be heavily contaminated with microorganisms and are a potential source of infection for both practicing staff and immunocompromised patients particularly. Contamination of dental unit water lines could be inhibited with the use of disinfectants. The present study investigates the effect of aloe-vera-based disinfectant in reducing the microbial growth in dental unit water lines (DUWLs. Aims. To compare the efficacy of aloe vera, hydrogen peroxide (H2O2, and 5% sodium hypochlorite (NaOCl in controlling microbial contamination of DUWLs. Materials and Methods. After obtaining baseline water samples, the dental unit waterlines were treated with aloe vera, 10% hydrogen peroxide, and 5% sodium hypochlorite. Each of the three disinfectants was used in increasing concentrations and their inhibiting effect was compared. Water samples were analyzed for microbiological quality by the total viable count (TVC method. Statistical Analysis Used. SPSS 16. Results. There was significant reduction in mean CFU/ml when treated with disinfectants each for a period of one week. Aloe-vera solution was found to be the most effective in reducing the microbial colonies. Conclusions. Improving the water quality from dental unit water lines is of considerable importance; chemical-based disinfectants can be replaced with herbal disinfectants for treating microbial contamination in dental unit waterlines.

  20. Aloe vera for prevention of radiation-induced dermatitis: a self-controlled clinical trial.

    Science.gov (United States)

    Haddad, P; Amouzgar-Hashemi, F; Samsami, S; Chinichian, S; Oghabian, M A

    2013-08-01

    To evaluate an Aloe vera lotion for prevention of radiation-induced dermatitis, all patients with a prescription of radiotherapy to a minimum dose of 40 Gy were eligible provided that their treatment area could be divided into two symmetrical halves. Patients were given a lotion of Aloe vera to use on one half of the irradiated area, with no medication to be used on the other half. The grade of dermatitis in each half was recorded weekly until 4 weeks after the end of radiotherapy. The trial enrolled 60 patients (mean age: 52 years; 67% women). Most patients had breast cancer (38%), followed by pelvic (32%), head-and-neck (22%), and other cancers (8%). Field size was 80-320 cm(2) (mean: 177 cm(2)), and the dose of radiotherapy was 40-70 Gy (mean: 54 Gy). Concurrent chemotherapy was administered in 20 patients. From week 4 to week 6 of radiotherapy and then at weeks 2 and 4 after radiotherapy, the mean grade of dermatitis with and without Aloe vera was 0.81 and 1.10 (p Aloe vera reduces the intensity of radiationinduced dermatitis.

  1. In vitro phytochemical characteristics and antioxidant capacity of Aloe vera, Plukenetia volubilis, Caiophora carduifolia, Cecropia membranacea

    OpenAIRE

    Franco Quino, César; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Laboratorio de Fisiología y Farmacología, Facultad de Odontología, UNMSM, Lima, Perú; Muñoz Espinoza, Danny; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Facultad de Ciencias Químicas, Universidad de Guayaquil, Ecuador; Gómez Herreros, César; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Chau Miranda, Gina; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Cueva Piña, Liliana; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Guardia Ortiz, Eliza; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Saavedra Yucra, Santos; Unidad de Posgrado, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos, Lima, Perú; Arroyo Acevedo, Jorge; Laboratorio de Farmacología Experimental, Facultad de Medicina Humana, UNMSM, Lima, Perú; Instituto de Investigaciones Clínicas, UNMSM, Lima, Perú; Herrera Calderón, Oscar; Facultad de Fisiología y Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad Nacional San Luis Gonzaga, Ica, Perú

    2016-01-01

    Introduction. Antioxidants have shown chemopreventive potential in degenerative, inflammatory, autoimmune, oncology and respiratory distress associated pathologies. Objective. To assess the phytochemical and antioxidant properties in vitro by DPPH and ABTS method. Design. Observational analytical. Location. Laboratory of Experimental Pharmacology, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological/Chemical Material. Aloe vera leaves, Plukenetia volubilis see...

  2. Effects of Aloe vera on dressing percentage and haemato-biochemidal parameters of broiler chickens

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    Jagmohan Singh

    2013-09-01

    Full Text Available Aim: To evaluate the effects of Aloe vera on dressing percentage and hemato-biochemical parameters of broiler chickens.Materials and Methods: A total of 90 chicks were used in this study. They were randomly allocated into 3 treatment groups.Fresh Aloe vera leaf juice (ALJ was prepared and administered to the test group T3 at the rate of 20 g/Lin drinking water daily.This study was carried out for 42 days. Dressing percentage and hemato-biochemical parameters were recorded at the end ofexperiment.Results: Group that was given Aloe vera (T3 showed numerically higher dressing percentage as compared to control group(T1 and drug control group (T2. It also showed significantly (P0.05 differences were observed in other parameters among all the treatment groups.Conclusion: Aloe vera has potential to be a growth promoter in broiler chicks and its growth promoting effects are comparableto that of antibiotic growth promoter (AGP.

  3. Expression of biologically active human interferon alpha 2 in aloe vera

    Science.gov (United States)

    We have developed a system for transgenic expression of proteins in Aloe Vera. Using this approach we have generated plants expressing the human gene interferon alpha 2, IFNa2. IFNa2 is a small secreted cytokine that plays a vital role in regulating the body’s immune response to viral infections a...

  4. Facile fabrication of aloe vera containing PCL nanofibers for barrier membrane application.

    Science.gov (United States)

    Carter, Princeton; Rahman, Shekh M; Bhattarai, Narayan

    2016-01-01

    Guided tissue regeneration (GTR) is a widely used method in dental surgical procedures that utilizes a barrier membrane to exclude migration of epithelium and ensure repopulation of periodontal ligament cells at the sites having insufficient gingiva. Commercial GTR membranes are typically composed of synthetic polymers that have had mild clinical success mostly because of their lack of proper bioactivity and appropriate degradation profile. In this study, a natural polymer, aloe vera was blended with polycaprolactone (PCL) to create nanofibrous GTR membranes by electrospinning. Aloe vera has proven anti-inflammatory properties and enhances the regeneration of periodontium tissues. PCL, a synthetic polymer, is well known to produce miscible polyblends nanofibers with natural polymers. Nanofibrous membranes with varying composition of PCL to aloe vera were fabricated, and several physicochemical and biological properties, such as fiber morphology, wettability, chemical structure, mechanical strength, and cellular compatibility of the membranes were analyzed. PCL/aloe vera membranes with ratios from 100/00 to 70/30 showed good uniformity in fiber morphology and suitable mechanical properties, and retained the integrity of their fibrous structure in aqueous solutions. Experimental results, using cell viability assay and cell attachment observation, showed that the nanofibrous membranes support 3T3 cell viability and could be a potential candidate for GTR therapy.

  5. Evaluation of antimicrobial efficacy of Aloe vera and its effectiveness in decontaminating gutta percha cones

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    Prakash P Athiban

    2012-01-01

    Results: The zones of inhibition on the agar plate were measured as 24mm,21mm and 24mm respectively. The broth remained clear even after 48 hours of incubation. Conclusion: We conclude that Aloe vera is indeed effective as a GP decontaminant and it holds a promising future as a medium for storage of GP cones.

  6. Immunostimulatory and protective effects of Aloe vera against coccidiosis in industrial broiler chickens.

    Science.gov (United States)

    Akhtar, Masood; Hai, Abdul; Awais, Mian Muhammad; Iqbal, Zafar; Muhammad, Faqir; ul Haq, Ahsan; Anwar, Muhammad Irfan

    2012-05-25

    This paper reports the immunostimulatory and protective effects of Aloe vera extracts (aqueous and ethanolic) against coccidiosis in industrial broiler chickens. The study was divided into two experiments. Experiment-I was conducted for the evaluation of immunostimulatory activity of A. vera and experiment-II demonstrated the protective efficacy of A. vera extracts against coccidiosis in chickens. Results of the experiment-I revealed significantly higher (pvera as compared to those administered with aqueous extract and control group. Microplate haemagglutination assay for humoral response on day 7th and 14th post primary and secondary injections of sheep red blood cells (SRBCs) revealed significantly higher (pAloe extract as compared to the ethanolic extract administered chickens (45%). Mean oocysts per gram of droppings in the control group was significantly higher (pAloe extract showed a minimal mean lesion score (2.3) followed by those administered with ethanolic Aloe extract (2.6) and control chickens (3.05) for caeca, and a similar pattern was observed for intestinal lesion scoring. Further, significantly higher weight gains and antibody titers (pvera extracts as compared to those in the control group. It was concluded that A. vera may be a potential and valuable candidate to stimulate the immune responses and can be used successfully as an immunotherapeutic agent against coccidiosis in industrial broiler chickens.

  7. Review of Clinical Pharmacology of Aloe vera L. in the Treatment of Psoriasis.

    Science.gov (United States)

    Miroddi, Marco; Navarra, Michele; Calapai, Fabrizio; Mancari, Ferdinando; Giofrè, Salvatore Vincenzo; Gangemi, Sebastiano; Calapai, Gioacchino

    2015-05-01

    Aloe vera L., is a plant used worldwide as folk remedy for the treatment of various ailments, including skin disorders. Its gel is present in cosmetics, medicinal products and food supplements. Psoriasis, an immune-mediated chronic inflammatory disease, involving mainly the skin, affects about the 2-3% of general population. Conventional pharmacological treatments for psoriasis can have limited effectiveness and can cause adverse reactions. For this reason often psoriatic patients look for alternative treatments based on natural products containing Aloe vera. We conducted a systematic review of clinical trials assessing effectiveness and safety of aloe for the treatment of psoriasis. Clinical studies published in English were considered; a total of four clinical trials met inclusion criteria. Studies were also evaluated by using the Jadad scale and Consort Statement in Reporting Clinical trials of Herbal Medicine Intervention. Quality and methodological accuracy of considered studies varied considerably, and some crucial information to reproduce clinical results was missing. We conclude that administration of aloe as cutaneous treatment is generally well tolerated, as no serious side effects were reported. Results on the effectiveness of Aloe vera are contradictory; our analysis reveals the presence of methodological gaps preventing to reach final conclusions.

  8. The increasing of fibroblast growth factor 2, osteocalcin, and osteoblast due to the induction of the combination of Aloe vera and 2% xenograft concelous bovine

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    Utari Kresnoadi

    2012-12-01

    Full Text Available Background: To make a successfull denture prominent ridge is needed, preservation on tooth extraction socket is needed in order to prevent alveol bone resorption caused by revocation trauma. An innovative modification of the material empirically suspected to be able reduce inflammation caused by the revocation trauma is a combination of Aloe vera and xenograft concelous bovine (XCB and Aloe vera is a biogenic stimulator and accelerating the growth of alveolar ridge bone after tooth extraction. Purpose: The research was aimed to determine of the increasing alveol bone formation by inducing the combination of Aloe vera and 2% xenograft concelous bovine. Methods: To address the problems, the combination of Aloe vera and xenograft concelous bovine was induced into the tooth extraction sockets of Cavia cabayas which devided on 8 groups. Groups control, filled with XCB, Aloe vera and Aloe vera and XCB combination, at 7 days and 30 days after extraction. Afterwards, immunohistochemical examination was conducted to examine the expressions of FGF-2 and osteocalcin, as the product of the growth of osteoblasts. Results: There were significantly increases expression of FGF-2 and osteocalcyn on group which filled with XCB, Aloe vera and combined Aloe vera and XCB. Conclusion: It may be concluded that the induction of the combination of Aloe vera and xenograft concelous bovine into the tooth sockets can enhance the growth expressions of FGF-2 and osteocalcin as the product of osteoblasts, thus, the growth of alveolar bone was increased.Latar belakang: Untuk keberhasilan pembuatan gigitiruan diperlukan ridge yang prominent, maka diperlukan suatu preservasi soket pencabutan gigi untuk mencegah terjadinya resopsi tulang alveolar akibat trauma pencabutan. Suatu inovasi modifikasi bahan yang diduga secara empiris dapat mengurangi keradangan karena trauma pencabutan adalah berupa kombinasi Aloe vera dan xenograft concelous bovine (XCB. Aloe vera yang merupakan

  9. Effect of crosslinking in chitosan/aloe vera-based membranes for biomedical applications.

    Science.gov (United States)

    Silva, S S; Caridade, S G; Mano, J F; Reis, R L

    2013-10-15

    The positive interaction between polysaccharides with active phytochemicals found in medicinal plants may represent a strategy to create active wound dressing materials useful for skin repair. In the present work, blended membranes composed of chitosan (Cht) and aloe vera gel were prepared through the solvent casting, and were crosslinked with genipin to improve their properties. Topography, swelling, wettability, mechanical properties and in vitro cellular response of the membranes were investigated. With the incorporation of aloe vera gel into chitosan solution, the developed chitosan/aloe-based membranes displayed increased roughness and wettability; while the genipin crosslinking promoted the formation of stiffer membranes in comparison to those of the non-modified membranes. Moreover, in vitro cell culture studies evidenced that the L929 cells have high cell viability, confirmed by MTS test and calcein-AM staining. The findings suggested that both blend compositions and crosslinking affected the physico-chemical properties and cellular behavior of the developed membranes.

  10. Diffusion of hydroxyl ions from calcium hydroxide and Aloe vera pastes.

    Science.gov (United States)

    Batista, Victor Eduardo de Souza; Olian, Douglas Dáquila; Mori, Graziela Garrido

    2014-01-01

    This study evaluated the diffusion through the dentinal tubules of hydroxyl ions from different calcium hydroxide (CH) pastes containing Aloe vera. Sixty single-rooted bovine teeth were used. The tooth crowns were removed, the root canals were instrumented and the specimens were assigned to 4 groups (n=15) according to the intracanal medication: Group CH/S - CH powder and saline paste; Group CH/P - CH powder and propylene glycol paste; Group CH/A - calcium hydroxide powder and Aloe vera gel paste; Group CH/A/P - CH powder, Aloe vera powder and propylene glycol paste. After placement of the root canal dressings, the teeth were sealed coronally and apically with a two-step epoxy adhesive. The teeth were placed in identified flasks containing deionized water and stored in an oven with 100% humidity at 37 °C. After 3 h, 24 h, 72 h, 7 days, 15 days and 30 days, the deionized water in the flasks was collected and its pH was measured by a pH meter. The obtained data were subjected to statistical analysis at a significance level of 5%. The results demonstrated that all pastes provided diffusion of hydroxyl ions through the dentinal tubules. The combination of Aloe vera and CH (group CH/A) provided a constant release of calcium ions. Group CH/A/P showed the highest pH at 24 and 72 h. In conclusion, the experimental pastes containing Aloe vera were able to enable the diffusion of hydroxyl ions through the dentinal tubules.

  11. In-vitro assessment and pharmacodynamics of nimesulide incorporated Aloe vera transemulgel.

    Science.gov (United States)

    Vandana, K R; Yalavarthi, Prasanna R; Sundaresan, C R; Sriramaneni, Raghava N; Vadlamudi, Harini C

    2014-06-01

    The aim of the investigation was to prepare nimesulide emulsion for incorporation in Aloe vera gel base to formulate 'nimesulide - Aloe vera transemulgel' (NAE) and to carryout in-vitro assessment and in-vivo anti-inflammatory studies of the product. Although the use of nimesulide is banned for oral administration, due to its potential for inducing hepatotoxicity and thrombocytopenia, the use of nimesulide for topical delivery is prominent in the treatment of many inflammatory conditions including rheumatoid arthritis. The drug loading capacity of transdermal gels is low for hydrophobic drugs such as nimesulide. Nimesulide can be effectively incorporated into emulgels (a combination of emulsion and gel). Aloe vera has a mild anti-inflammatory effect and in the present study Aloe vera gel was formulated and used as a gel base to prepare NAE. The emulgels thus prepared were evaluated for viscosity, pH, in-vitro permeation, stability and skin irritation test. In-vivo anti-inflammatory studies were performed using carrageenan induced hind paw edema method in Wistar rats. The results were compared with that of commercial nimesulide gel (CNG). From the in-vitro studies, effective permeation of nimesulide from NAE (53.04 %) was observed compared to CNG (44.72 %) at 30 min indicating better drug release from NAE. Topical application of the emulgel found no skin irritation. Stability studies proved the integrity of the formulation. The percentage of inhibition of edema was highest for the prepared NAE (67.4 % inhibition after 240 min) compared to CNG (59.6 %). From our results, it was concluded that the Aloe vera gel acts as an effective gel base to prepare nimesulide emulgel with high drug loading capacity (86.4 % drug content) compared to CNG (70.5 % drug content) with significant anti-inflammatory effect.

  12. Clinical Effectiveness of Aloe Vera in the Management of Oral Mucosal Diseases- A Systematic Review

    Science.gov (United States)

    Nair, Gopakumar Ramachandran; Naidu, Giridhar Seetharam; Jain, Supreet; Makkad, Ramanpal Singh; Jha, Abhishek

    2016-01-01

    Introduction Aloe vera is well known for its medicinal properties which lead to its application in treating various diseases. Its use in treating oral lesions has not been much documented in literature. Aim Although, systematic reviews on aloe vera and its extracts have been done earlier, but in relation to oral diseases this is the first systematic review. The aim of the present systematic review was to compile evidence based studies on the effectiveness of Aloe vera in treatment of various oral diseases. Materials and Methods Computerized literature searches were performed to identify all published articles in the subject. The following databases were used: PUBMED [MEDLINE], SCOPUS, COCHRANE DATABASE, EMBASE and SCIENCE DIRECT using specific keywords. The search was limited to articles published in English or with an English Abstract. All articles (or abstracts if available as abstracts) were read in full. Data were extracted in a predefined fashion. Assessment was done using Jadad score. Results Fifteen studies satisfied the inclusion criteria. Population of sample study ranged from 20 patients to 110 patients with clinically diagnosed oral mucosal lesions. Out of 15 studies, five were on patients with oral lichen planus, two on patients with oral submucous fibrosis, other studies were carried on patients with burning mouth syndrome, radiation induced mucositis, candida associated denture stomatitis, xerostomic patients and four were on minor recurrent apthous stomatitis. Most studies showed statistically significant result demonstrating the effectiveness of Aloe vera in treatment of oral diseases. Conclusion Although there are promising results but in future, more controlled clinical trials are required to prove the effectiveness of Aloe vera for management of oral diseases. PMID:27656587

  13. High-performance nanothermite composites based on aloe-vera-directed CuO nanorods.

    Science.gov (United States)

    Patel, Vinay Kumar; Bhattacharya, Shantanu

    2013-12-26

    In this work, we demonstrate the development of high-performance nanothermite composites derived from super-reactive CuO nanorods oxidizers fabricated by simple biogenic routes using Aloe vera plant extracts. Nanorods of various length scales have been realized via simple sonoemulsion and solid-state biosynthesis routes using Aloe vera gel as a green surfactant promoting the directional growth of CuO nanorods in both solid and emulsion phase. The biosynthesized CuO nanorods (oxidizers)/fuel (nanoaluminum) composites ignited vigorously with abundant gas generation, developing high heat of reaction of 1.66 kJ g(-1) and very high pressurization rate of around 1.09 MPa μs(-1) and peak pressure of 65.4 MPa when blasted inside a constant volume pressure cell with a charge density of 0.2 g cm(-3). The pressurization rates so obtained are four times higher with twice the peak pressure in comparison to such nanothermites formulated via other available state of the art wet-chemical techniques, which reflects the catalytic role of Aloe vera surface functional groups (A. vera-sfg) enhancing the reactivity of CuO oxidizers with excess gas release rate during exothermic reaction with nanoaluminum. Through this work, Aloe vera gel has for the first time been identified as a novel biotemplate for green synthesis of nanorod structures of metal oxides, and we have also studied the utility of A. vera-sfg in the creation of super-reactive CuO oxidizers producing excellent heat of reaction and dynamic pressure characteristics as demanded in propellants, explosives, and pyrotechnics.

  14. Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broilers

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    Ali Mahdavi

    2011-07-01

    Full Text Available The present study attempts to analyze the effects of different levels of Aloe vera gel as an alternative to antibiotic, on performance and ileum morphology in broilers. Three hundred one-day old Ross 308 male broilers were used on a completely randomized design in 5 groups with 4 replicates, each consisting of 15 broilers. The groups included the control group (basal diet and three groups with basal diet mixed with different levels of Aloe vera gel (1.5%, 2% and 2.5%. Finally, there was a group with basal diet plus 15 ppm antibiotic virginiamycin. The results obtained regarding performance of the broilers showed that Aloe vera gel groups brought about higher body weight gain and feed intake compared to the control group; however, no significant difference was observed in feed conversion ratio between the groups treated by Aloe vera gel and the control group (P>0.05. Although the antibiotic group showed better performance and heavier dressing percentage than the Aloe vera gel and the control groups, no significant difference was seen between the group treated by 2% Aloe vera gel and the antibiotic group regarding body weight gain, feed conversion ratio and dressing percentage (P>0.05. Among the Aloe vera gel groups, the 2% Aloe vera gel group had the largest villus height and the greatest villus height to crypt depth ratio compared to the antibiotic group (PAloe vera gel treatment may be recommended to achieve the best performance in broilers as an alternative to antibiotic growth promoter.

  15. Caracterización morfoanatómica comparativa entre Aloe vera (L. Burm. F., Aloe arborescens Mill., Aloe saponaria Haw. y Aloe ciliaris Haw. (Aloeaceae

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    Stella Maris Carpano

    2009-03-01

    Full Text Available Aloe vera (L. Burm. F. (= Aloe barbadensis Miller conhecida como "a planta da imortalidade" no antigo Egito é utilizada em fitoterapia como humectante, antibacteriana, antifúngica, antiviral e antioxidante. Com a finalidade de contribuir para identificação da droga vegetal, foram realizados estudos morfoanatômicos da folha. A utilização de plantas com características terapêuticas reconhecidas determina que, por analogia, popularmente sejam utilizadas com a mesma finalidade outras espécies do mesmo gênero. Isto é o que ocorre com três espécies de Aloe spp., que se cultivam ou crescem acidentalmente na Argentina: A. arborescens Mill., A. saponaria Haw. e A. ciliaris Haw. (Aloaceae. Estabeleceram-se os caracteres morfoanatômicos de diagnóstico por microscopia óptica e microscopia eletrônica de varredura (apresentam-se desenhos e fotomicrografias dos mesmos. Obtiveram-se valores numéricos dos elementos histológicos de diagnóstico: magnitudes lineares e proporcionais. Realizaram-se reações histoquímicas de identificação dos princípios ativos para sua localização "in situ".

  16. Aloe vera and Vitis vinifera improve wound healing in an in vivo rat burn wound model.

    Science.gov (United States)

    Lin, Li-Xin; Wang, Peng; Wang, Yu-Ting; Huang, Yong; Jiang, Lei; Wang, Xue-Ming

    2016-02-01

    Aloe vera and Vitis vinifera have been traditionally used as wound healing agents. The present study aimed to investigate the effects of aloe emodin and resveratrol in the burn wound healing procedure. Burn wounds are common in developed and developing countries, however, in developing countries, the incidence of severe complications is higher and financial resources are limited. The results of the present study demonstrated that neither aloe emodin or resveratrol were cytotoxic to THP-1 macrophages at concentrations of 1, 100 and 500 ng/ml. A significant increase in wound-healing activity was observed in mice treated with the aloe emodin and resveratrol, compared with those which received control treatments. The levels of IL-1β in the exudates of the burn wound area of the treated mice increased in a time-dependent manner over 7 days following burn wound injury. At 10 days post-injury, steady and progressive wound healing was observed in the control animals. The present study confirmed that increased wound healing occurs following treatment with aloe emodin,, compared with resveratrol, providing support for the use of Aloe vera plants to improve burn wound healing.

  17. Evaluation of radioprotective effect of aloe vera and zinc/copper compounds against salivary dysfunction in irradiated rats.

    Science.gov (United States)

    Nejaim, Yuri; I V Silva, Amaro; V Vasconcelos, Taruska; J N L Silva, Emmanuel; M de Almeida, Solange

    2014-09-01

    The aim of this study was to evaluate the radioprotective and reparative effects of compounds based on aloe vera, zinc, and copper against salivary gland dysfunction in Wistar rats. A total of 150 Wistar rats were randomly divided into 12 groups, in which the animals received aloe vera and/or zinc and copper. In eight of these groups the animals were also subjected to irradiation before or after administration of the substances. After 27 days, sialometry tests were performed. Data were analyzed using ANOVA and the Tukey test (P aloe vera before or after irradiation showed a significantly higher salivary flow rate than rats that had been simply irradiated. When both substances were administered, a statistically significant difference in the salivary flow rate was observed in comparison with the irradiation alone group seven days after irradiation. The present results suggest that aloe vera exerts positive protective and reparative effects, and can be considered a potential radioprotective substance.

  18. A Comparison between Antibacterial Activity of Propolis and Aloe vera on Enterococcus faecalis (an In Vitro Study)

    OpenAIRE

    Ehsani, Maryam; Amin Marashi, Mahmood; Zabihi, Ebrahim; Issazadeh, Maryam; Khafri, Soraya

    2013-01-01

    Removing the bacteria, including Enterococcus faecalis, from the root canal is one of the important aims in endodontic treatment.We aimed to compare the antibacterial activity of Chlorhexidine with two natural drugs. The antibacterial activities of three different propolis extracts (alcohol concentrations: 0, 15, 40%) and Aloe vera gel on E. faecalis were compared using three methods: disk diffusion, microdilution and direct contact test. In addition to the above bacterium, the Aloe vera gel ...

  19. Economic Sustainability of Organic Aloe Vera Farming in Greece under Risk and Uncertainty

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    Angelos Liontakis

    2016-04-01

    Full Text Available During the last decade, an encouraging environment for the restructuring and modernization of the agricultural sector has formed in Greece. The diversification into higher-value crops can be a promising option for small and average-sized farms, particularly during the current economic crisis. One of the most promising alternative crops that have been recently established in Greece is the organic Aloe vera crop. The main advantage of this crop is that it can utilize poor farmlands and, therefore, can facilitate rural development in marginal areas. This study explores the economic sustainability of the Aloe vera crop, considering the embedded risk and uncertainty. The results indicate that organic aloe farming is a promising alternative to “traditional” crops in Greece, particularly for family farms in rural areas. In contrast, this activity is not advisable to the most entrepreneurial type of farmers, unless their crop size allows economies of scales. Finally, the Stochastic Efficiency with Respect to a Function (SERF analysis associates farmers’ risk attitude with their willingness to be involved in organic Aloe vera farming. SERF analysis highlights the crucial role of farmers’ risk aversion and concludes that, above a certain level of risk aversion, farmers have no incentive to adopt this economic activity.

  20. Standardization of micropropgation techniques for Aloe vera: a pharmaceutically important plant.

    Science.gov (United States)

    Zakia, Shehnaz; Zahid, Najma Yousaf; Yaseen, Mehwish; Abbasi, Nadeem Akhtar; Hafiz, Ashfaq Ahmad; Mahmood, Nasir

    2013-11-01

    Aloe vera (Syn Aloe barbadensis Mill.), a medicinal plant, has a great potential in cosmetic and drug industry due to presence of more than 200 bioactive compounds. Natural propagation of Aloe vera, by means of suckers, is very slow and insufficient to meet the increasing demand of pharmaceutical and cosmetic industries. Shoot tip was used as an explant for in vitro regeneration of Aloe vera. Explants were disinfested with the use of 0.1% mercuric chloride and 0.5% sodium hypochlorite, and washed thoroughly with autoclaved distilled water. Solid MS medium was used with addition of different concentrations of 6-benzyl aminopurine and α-naphthalene acitic acid. After 7 weeks of inoculation, greatest number of shoots (11.18) and highest shoot length (12.15cm) were found in MS medium supplemented with 0.5 mg l-1 6-benzylaminopurine (BAP) along with same concentration of α-naphthalene acitic acid (NAA). Best rooting (84.67%) was found in medium supplemented with 1.5 mg l-1 of indole butyric acid (IBA). The rooted explants were then gradually acclimatized and shifted to green house.

  1. Study of trace metals concentration and antimicrobial properties of tropical Aloe vera plant from southern India

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    V. Subramani

    2014-06-01

    Full Text Available This study was carried out with an objective to investigate the antibacterial and antifungal potentials and trace metals concentrations in Aloe vera (Linn plant leaves. Fresh leaves of Aloe vera were collected from Tiruchirappalli district of Tamil Nadu during the period of February - March 2014. The 100 g of shade dried A. vera leave power was used to collect the methanol extraction of the test plant by the soxhlet apparatus. The extracted solutions were dried by hot air oven at 60 °C for 48-72 h for further analysis. The antimicrobial activity of Aloe vera methanol extract was examined with six various pathogenic microorganisms such as gram positive, gram negative and fungal strains using the disk diffusion test. The two tested concentrations such as 0.60 and 1.20 mg/disc produce zone of inhibition on muller hinton agar (MHA and potato dextrose agar (PDA plates for bacteria and fungi, respectively. In this study, higher (1.20 mg concentration got greater sensitivity than lower (0.60 mg concentration against all strains. All the microbial strains depict higher sensitivity to the higher concentration (1.2 mg / disc for the test sample when compared to the positive control except bacterial strains such as Aeromonas liquefaciens MTCC 2645 (B1. The trace metal analyses of the plants were also carried out. The mean concentration of trace metals such as cadmium (Cd, chromium (Cr, copper (Cu, iron (Fe, nickel (Ni, lead (Pb and zinc (Zn were 0.04, BDL, 0.06, 0.08, BDL, 0.02 and 0.22 mg kg-1, respectively. Therefore, it is signified that Aloe vera plant extract is safe to be used as an antimicrobial agent. Hence, throughout impoundment is needed to verify the trace metal levels in plants.

  2. Physicochemical characterization of silver nanoparticles synthesize using Aloe Vera (Aloe barbadensis)

    Science.gov (United States)

    Kuponiyi, Abiola; Kassama, Lamin; Kukhtareva, Tatiana

    2014-08-01

    Production of silver nanoparticles (AgNPs) using different biological methods is gaining recognition due to their multiple applications. Although, several physical and chemical methods have been used for the synthesis and stabilizing of AgNPs, yet, a green chemistry method is preferable because it is cost effective and environmentally friendly. The synthesis was done using Aloe Vera (AV) extract because it has chemical compounds such as "Antrokinon" that are known for its antibacterial, antivirus and anticancer properties. We hypothesize that AV extract can produce a stable nanoparticles within the 100 nm range and be biologically active. The biological compounds were extracted from AV skin with water and ethanol which was used as the reduction agent for the synthesis of nanoparticles. The biological extract and AgNO3 were blended and heated to synthesize AgNPs. The reaction process was monitored using UV-Visible spectroscopy. Fourier Transfer Infrared spectroscopy (FTIR) was used for the characterization of biological compounds and their substituent groups before and after the reaction process. Dynamic Light scattering (DLS) method was used to characterize particle size of AgNPs and their biomolecular stability. Results showed that biological compounds such as aliphatic amines, alkenes (=C-H), alkanes (C-H), alcohol (O-H) and unsaturated esters(C-O), which has an average particle size of 109 and 215.8 nm and polydispersity index of 0.451 and 0.375 for ethanol and water extract, respectively. According to TEM measurements the size of AgNPs are in the range 5-20 nm The results suggested that ethanol derived AgNPs contained higher yield of organic compounds, thus has better solubility power than water. Ag NPs can be used to control salmonella in poultry industry.

  3. Hypoglycemic and Hypolipidemic Effects of Aloe vera Extract Preparations: A Review.

    Science.gov (United States)

    Pothuraju, Ramesh; Sharma, Raj Kumar; Onteru, Suneel Kumar; Singh, Satvinder; Hussain, Shaik Abdul

    2016-02-01

    Obesity is considered to be an epidemic disease, and it is associated with several metabolic disorders. Pharmacological treatments currently available are not effective for prolonged treatment duration. So, people are looking toward new therapeutic approach such as herbal ingredients. Since ancient periods, different herbs have been used for remedy purposes such as anti-obesity, antidiabetes, and antiinflammatory. Among the several herbal ingredients, Aloe vera (Aloe barbadensis Miller) is widely used to curb the metabolic complications. Till date, reports are not available for the side effects of A. vera. Several researchers are used to different solvents such as aqueous solution, alcohol, ethanol, and chloroform for the A. vera extract preparations and studied their hypoglycemic and hypolipidemic effects in animal and human studies. Furthermore, little information was recorded with the active compounds extracted from the A. vera and their anti-obesity and antidiabetic effects in clinical studies. In this review, we made an attempt to compile all the available literature by using different search engines (PubMed, Scopus, and Google Scholar) on the A. vera extract preparations and the possible mechanism of action involved in carbohydrate and lipid metabolism.

  4. Bioaktivitas Gel Aloe vera pada Gonad Tikus Putih Jantan (Rattus norvegicus

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    I Ketut Suardita

    2013-11-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE The purpose of this study was to determine Bioactivity of Aloe vera gel on gonad of male white rats (Rattus norvegicus. This research used complately random design, with three treatments namely T0, received placebo as a control; T1, received gel of Aloe vera 300 mg/kg body weight orally; and T2, received gel of Aloe vera 400 mg/kg body weight orally. Aloe vera gel was administrated every day for 21 days. All the treatments repeated three times, and each experimental unit used one white rat. Data found was analyzed using one way of Anova, and the Least Significant Differences (LSD Test was applied for further analysis. Results showed that Aloe vera gel administration with a dose of 300 mg/kg body weight had effected significantly on the number of spermatogonia cells, live-daed rasio of spermatozoa, and the percentage of sperm abnormality. The result of the research indicates that Aloe vera gel is potentially cytotoxic to testes cells, and so, it’has a high possibilities as an alternative herbal contraceptive agent for animals /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; text-align:justify; line-height:150%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin;}

  5. Feeding mice with Aloe vera gel diminishes L-1 sarcoma-induced early neovascular response and tumor growth.

    Science.gov (United States)

    Kocik, Janusz; Bałan, Barbara Joanna; Zdanowski, Robert; Jung, Leszek; Skopińska-Różewska, Ewa; Skopiński, Piotr

    2014-01-01

    Aloe vera (Aloe arborescens, aloe barbadensis) is a medicinal plant belonging to the Liliaceae family. Aloe vera gel prepared from the inner part of Aloe leaves is increasingly consumed as a beverage dietary supplement. Some data suggest its tumor growth modulatory properties. The aim of the present study was to evaluate in Balb/c mice the in vivo influence of orally administered Aloe vera drinking gel on the syngeneic L-1 sarcoma tumor growth and its vascularization: early cutaneous neovascular response, tumor-induced angiogenesis (TIA test read after 3 days), and tumor hemoglobin content measured 14 days after L-1 sarcoma cell grafting. Feeding mice for 3 days after tumor cell grafting with 150 μl daily dose of Aloe vera gel significantly diminished the number of newly-formed blood vessels in comparison to the controls. The difference between the groups of control and Aloe-fed mice (150 μl daily dose for 14 days) with respect to the 14 days' tumor volume was on the border of statistical significance. No difference was observed in tumor hemoglobin content.

  6. ALOE-VERA GEL AS POTENTIAL CORROSION INHIBITOR FOR CONCRETE STEEL REINFORCEMENT

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    Héctor Herrera-Hernández

    2015-09-01

    Full Text Available In this work a GEL extracted from ALOE VERA leaves has been electrochemically studied as a possible corrosion inhibitor for concrete steel rebar. The corrosion resistance was evaluated by Electrochemical Impedance Spectroscopy (EIS measurements in hydrochloric acid solution (1M HCl as a function of GEL-inhibitor addition, uninhibited measurements were also discussed. The impedance results revealed that after addition of different GEL-extract proportion, the corrosion process of the steel exposed to acidic solution is remarkably inhibited, and it was also determined that the molecules of the GEL-extract follow a physisorption mechanism on the metal surface according to the Langmuir isotherm model with and adsorption standard free energy of about G°ads 14.17 kJ/mol. In this sense, the GEL extracted from ALOE-VERA leaves behaved as a mixed-type inhibitor.

  7. Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

    Energy Technology Data Exchange (ETDEWEB)

    Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean [Center of Physics and Material Studies, Faculty of Applied Sciences, Universiti Teknologi Mara (UiTM) 40450 Shah Alam, Selangor (Malaysia)

    2015-08-28

    Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140–260nm.

  8. Role of Natural Immunomodulator (Aloe Vera in Cellular and Humoral Immune Responses

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    Ening Wiedosari

    2007-12-01

    Full Text Available Aloe vera belongs to a group of Liliaceae family plant and cultivated worldwide. It possesses acemannan (acetylated mannan, which has a significant pharmacological property. The acemannan has an immunomodulatory activity when administered to animals. The major immunomodulating effect includes the activation of immune effector cells, such as lymphocytes and macrophages, resulting in the production of cytokines, interleukin (IL-1, IL-6, IL-12 and tumor necrosis factor alpha (TNFα. In particular, this extract can modulate the differentiation capacity of CD4+T cells to mature into Th1 subsets and enhance the innate cytokine response. As a consequence, this extract will have a profound effect in controlling disease, caused by intracellular infectious agents (bacteria and viruses. However, further studies are needed to determine the immunomodulating effects of Aloe vera in multi-component extracts equivalent to what are being used commonly in traditional medicine.

  9. Impedance studies of a green blend polymer electrolyte based on PVA and Aloe-vera

    Science.gov (United States)

    Selvalakshmi, S.; Mathavan, T.; Vijaya, N.; Selvasekarapandian, Premalatha, M.; Monisha, S.

    2016-05-01

    The development of polymer electrolyte materials for energy generating and energy storage devices is a challenge today. A new type of blended green electrolyte based on Poly-vinyl alcohol (PVA) and Aloe-vera has been prepared by solution casting technique. The blending of polymers may lead to the increase in stability due to one polymer portraying itself as a mechanical stiffener and the other as a gelled matrix supported by the other. The prepared blend electrolytes were subjected to Ac impedance studies. It has been found out that the polymer film in which 1 gm of PVA was dissolved in 40 ml of Aloe-vera extract exhibits highest conductivity and its value is 3.08 × 10-4 S cm-1.

  10. Preparation and characterization of chitosan/Aloe Vera composite nanofibers generated by electrostatic spinning

    Science.gov (United States)

    Ibrahim, Illani; Sekak, Khairunnadim Ahmad; Hasbullah, Norazurean

    2015-08-01

    Researches on the fabrication of nanostructured based membrane have attracted great attention amongst scientists due to their wide potential applications on medical application. In this work, Chitosan and Aloe Vera sol-gel solution were electrospun using 20 kV DC supply at room temperature. Morphological structure and functional group of nanofibers were characterized using field emission scanning electron microscopy (FESEM) and Fourier-transform infrared spectroscopy (FT-IR) respectively. The optimum parameter obtained at 90% concentration of acetic acid, 0.3 ml/h of solution flow rate and 10 cm distance of nozzle to collector. The fiber diameters were analyzed using the ImageJ software. Average diameters of the Chitosan/Aloe Vera composite nanofibers is 183nm in ranges of 140-260nm.

  11. Aloe vera : Potential candidate in health management via modulation of biological activities

    Directory of Open Access Journals (Sweden)

    Arshad H Rahmani

    2015-01-01

    Full Text Available Treatment based on natural products is rapidly increasing worldwide due to the affordability and fewer side effects of such treatment. Various plants and the products derived from them are commonly used in primary health treatment, and they play a pivotal role in the treatment of diseases via modulation of biochemical and molecular pathways. Aloe vera, a succulent species, produces gel and latex, plays a therapeutic role in health management through antioxidant, antitumor, and anti-inflammatory activities, and also offers a suitable alternative approach for the treatment of various types of diseases. In this review, we summarize the possible mechanism of action and the therapeutic implications of Aloe vera in health maintenance based on its modulation of various biological activities.

  12. Anti-tumor activity of Aloe vera against DMBA/croton oil-induced skin papillomagenesis in Swiss albino mice.

    Science.gov (United States)

    Saini, M; Goyal, Pradeep Kumar; Chaudhary, Geeta

    2010-01-01

    Human populations are increasingly exposed to various carcinogens such as chemicals, radiation, and viruses in the environment. Chemopreventive drugs of plant origin are a promising strategy for cancer control because they are generally nontoxic or less toxic than synthetic che-mopreventive agents, and can be effective at different stages of carcinogenesis. The present investigation was undertaken to explore the antitumor activity of topical treatment with aloe vera (Aloe vera) gel, oral treatment with aloe vera extract, and topical and oral treatment with both gel and extract in stage-2 skin carcinogenesis in Swiss albino mice induced by 7,12-dim ethylbenz(a)anthracene (DMBA) and promoted croton (Croton tiglium) oil. The animals were randomly divided into 4 groups and treated as follows: Group I, DMBA + croton oil only (controls); Group II, DMBA + croton oil + topical aloe vera gel; Group III, DMBA + croton oil + oral aloe vera extract; Group I V, DMBA + croton oil + topical aloe vera gel + oral aloe vera extract. Results showed that body weight was significantly increased from 78.6% in the control group (Group I) to 92.5%, 87.5%, and 90.0% in Groups II, III, and I V, respectively. A 100% incidence of tumor development was noted in Group I, which was decreased to 50%, 60%, and 40% in Groups II, III, and I V, respectively. Also in Groups II, III, and IV, the cumulative number of papillomas was reduced significantly from 36 to 12, 15, and 11; tumor yield from 3.6 to 1.2, 1.5, and 1.1; and tumor burden from 3.6 to 2.4, 2.50, and 2.75, respectively, after treatment with aloe vera. Conversely, the average latent period increased significantly from 4.9 (Group I) to 5.23, 5.0, and 6.01 weeks in Groups II, III, and I V, respectively. We conclude that aloe vera protects mice against DMBA/croton oil-induced skin papillomagenesis, likely due to the chemopreventive activity of high concentrations of antioxidants such as vitamins A, C, and E; glutathione peroxidase; several

  13. In vitro activity of Aloe vera inner gel against microorganisms grown in planktonic and sessile phases.

    Science.gov (United States)

    Cataldi, V; Di Bartolomeo, S; Di Campli, E; Nostro, A; Cellini, L; Di Giulio, M

    2015-12-01

    The failure of traditional antimicrobial treatments is becoming a worldwide problem. The use of Aloe vera is of particular interest for its role as curative agent and its efficacy in complementary therapies for a variety of illnesses. This study evaluated the antimicrobial activity of A. vera inner gel against a panel of microorganisms, Gram-positive and -negative bacteria, and Candida albicans. In addition to A. vera inner gel being used in the treatment of peptic ulcers, in dermatological treatments, and wound healing, it was also tested on the sessile phase of clinical Helicobacter pylori strains (including multi-drug-resistant strains) and on planktonic and sessile phase of Staphylococcus aureus/Pseudomonas aeruginosa clinical isolates from venous leg ulcers.A. vera inner gel expresses its prevalent activity against Gram-negative bacteria and C. albicans in respect to Gram-positive bacteria. The results of the A. vera antibiofilm activity showed a decrease of the produced biomass in a concentration-dependent-way, in each analyzed microorganism. The data obtained show that A. vera inner gel has both an antimicrobial and antibiofilm activity suggesting its potential use for the treatment of microbial infections, in particular for H. pylori gastric infection, especially in case of multi-drug-resistance, as well as for an effective wound dressing.

  14. Formulation and evaluation of topical polyherbal antiacne gels containing Garcinia mangostana and Aloe vera

    OpenAIRE

    Gowda Bhaskar; Shariff Arshia; S.R.B Priyadarshini

    2009-01-01

    The objective of the study was to develop a topical poly herbal gel for the treatment of mild acne vulgaris. Aqueous extracts of Garcinia mangostana and Aloe vera were formulated in an aqueous based carbopol-934(1%w/w) gel system. Preformulation studies on solubility, partition co-efficient, MIC, MBC were determined along with compatibility studies using a validated HPLC method. Six formulations of the gel were prepared by varying the proportions of polymers and evaluated for their physicoche...

  15. Preparation and characterization of aloe vera blended collagen-chitosan composite scaffold for tissue engineering applications.

    Science.gov (United States)

    Jithendra, Panneerselvam; Rajam, Abraham Merlin; Kalaivani, Thambiran; Mandal, Asit Baran; Rose, Chellan

    2013-08-14

    Collagen-Chitosan (COL-CS) scaffolds supplemented with different concentrations (0.1-0.5%) of aloe vera (AV) were prepared and tested in vitro for their possible application in tissue engineering. After studying the microstructure and mechanical properties of all the composite preparations, a 0.2% AV blended COL-CS scaffold was chosen for further studies. Scaffolds were examined by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), and thermogravimetry analysis (TGA) to understand the intermolecular interactions and their influence on the thermal property of the complex composite. Swelling property in phosphate buffered saline (pH 7.4) and in vitro biodegradability by collagenase digestion method were monitored to assess the stability of the scaffold in a physiological medium in a hydrated condition, and to assay its resistance against enzymatic forces. The scanning electron microscope (SEM) image of the scaffold samples showed porous architecture with gradual change in their morphology and reduced tensile properties with increasing aloe vera concentration. The FTIR spectrum revealed the overlap of the AV absorption peak with the absorption peak of COL-CS. The inclusion of AV to COL-CS increased the thermal stability as well as hydrophilicity of the scaffolds. Cell culture studies on the scaffold showed enhanced growth and proliferation of fibroblasts (3T3L1) without exhibiting any toxicity. Also, normal cell morphology and proliferation were observed by fluorescence microscopy and SEM. The rate of cell growth in the presence/absence of aloe vera in the scaffolds was in the order: COL-CS-AV > COL-CS > TCP (tissue culture polystyrene plate). These results suggested that the aloe vera gel-blended COL-CS scaffolds could be a promising candidate for tissue engineering applications.

  16. Comparison of Plaque Inhibiting Efficacies of Aloe Vera and Propolis Tooth Gels: A Randomized PCR Study

    Science.gov (United States)

    Sunkara, Musalaiah Svv; Pantareddy, Indeevar; Sudhakar, Sankaran

    2015-01-01

    Backgound and Aim Allopathic medications used for periodontal disease are known to be associated with various side effects. Hence a search for naturotherapies are on the rise. Among the natural pharmacons available aloevera and propolis are considered to be effective and free from adverse effects. Taking this into account, the present study was done to compare the plaque inhibiting efficacies of Aloe vera and Propolis tooth gels in patients with chronic periodontitis. Materials and Methods Forty patients diagnosed with chronic periodontitis were randomly allocated to groups A and B containing 20 patients each. Patients in group A were advised to use Aloe vera tooth gel while those in group B were advised to use Propolis tooth gel. Clinical and microbiologic parameters using Polymerase chain reaction (PCR) were recorded at baseline and after 3 months. Results Student t-test was performed for all the obtained results. In the Aloe vera group, comparison of baseline PCR and after 3 month results showed reduction only in P. gingivalis (p=0.001), where as statistically significant reduction in all the three red complex microorganisms was seen in propolis group. All the clinical parameters (Plaque Index, Gingival Index, Bleeding on Probing, Probing pocket Depth, and Clinical Attachment Level) in both the groups showed statistically significant reductions after 3 months. Conclusion Propolis showed a statistically significant reduction in plaque, microbiologic and clinical parameters. However, clinical trials of longer durations with larger sample sizes are required to evaluate the efficacy. PMID:26501001

  17. Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel

    Energy Technology Data Exchange (ETDEWEB)

    Grindlay, D.; Reynolds, T.

    1986-06-01

    The mucilaginous gel from the parenchymatous cells in the leaf pulp of Aloe vera has been used since early times for a host of curative purposes. This gel should be distinguished clearly from the bitter yellow exudate originating from the bundle sheath cells, which is used for its purgative effects. Aloe vera gel has come to play a prominent role as a contemporary folk remedy, and numerous optimistic, and in some cases extravagant, claims have been made for its medicinal properties. Modern clinical use of the gel began in the 1930s, with reports of successful treatment of X-ray and radium burns, which led to further experimental studies using laboratory animals in the following decades. The reports of these experiments and the numerous favourable case histories did not give conclusive evidence, since although positive results were usually described, much of the work suffered from poor experimental design and insufficiently large test samples. In addition some conflicting or inconsistent results were obtained. With the recent resurgence of interest in Aloe vera gel, however, new experimental work has indicated the possibility of distinct physiological effects. Chemical analysis has shown the gel to contain various carbohydrate polymers, notably either glucomannans or pectic acid, along with a range of other organic and inorganic components. Although many physiological properties of the gel have been described, there is no certain correlation between these and the identified gel components. 154 references.

  18. Pengaruh Lidah Buaya (Aloe vera L. pada Paru-Paru Tikus yang Diinduksi Asap Rokok

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    Nur Atik

    2012-09-01

    Full Text Available The incident of smoking is one of the world’s health problems, as smoking increases the incidence of chronic obstructive pulmonary disease (COPD. Aloe vera L has anti-inflammatory, immunomodulator, and β-sitosterol properties. A research was conducted to observe its effects in preventing the cell apoptotic events as well as the increasing numbers of macrophages and the decreasing phagocytotic function of macrophages in rat’s lung (Rattus norvegicus that has been induced by cigarettes smoke. The study was conducted in January 2009–January 2010, located in Department of Cell Biology, Clinical Pathology, Anatomy Pathology and Health Research Unit of Faculty of Medicine University of Padjadjaran.This experimental study was conducted on rats which were divided into 3 groups each consisted 8 rats for 42 days in treatments. The data were collected from number of macrophages and its activity and also alveoli epithelial cell Bcl2 expression observation, than statistical analytic was performed using Mann-Whitney test. Result showed the significant difference (p<0.05 in number of macrophages, cigarette smoke group had a higher number (495.88±104.09 than cigarette smoke plus aloe group (295.63±176.79 but it showed the opposite for the phagocytotic function of macrophages. The cigarette smoke plus aloe group had higher percentage (11.67±12.84 than cigarette smoke group (3.77±1.39. Observation of Bcl2 expression in rat’s lungs alveoli cell showed that the group exposed to cigarettes and Aloe vera were more expressed compared with cigarette exposed group (p <0.05. This study proved that Aloe vera gel increases the number and cell activity of macrophages in addition to the Bcl2 expression in rat lungs which have been induced by cigarette smoke.

  19. Aloe vera downregulates LPS-induced inflammatory cytokine production and expression of NLRP3 inflammasome in human macrophages.

    Science.gov (United States)

    Budai, Marietta M; Varga, Aliz; Milesz, Sándor; Tőzsér, József; Benkő, Szilvia

    2013-12-01

    Aloe vera has been used in traditional herbal medicine as an immunomodulatory agent inducing anti-inflammatory effects. However, its role on the IL-1β inflammatory cytokine production has not been studied. IL-1β production is strictly regulated both at transcriptional and posttranslational levels through the activity of Nlrp3 inflammasome. In this study we aimed to determine the effect of Aloe vera on the molecular mechanisms of Nlrp3 inflammasome-mediated IL-1β production in LPS-activated human THP-1 cells and monocyte-derived macrophages. Our results show that Aloe vera significantly reduced IL-8, TNFα, IL-6 and IL-1β cytokine production in a dose dependent manner. The inhibitory effect was substantially more pronounced in the primary cells. We found that Aloe vera inhibited the expression of pro-IL-1β, Nlrp3, caspase-1 as well as that of the P2X7 receptor in the LPS-induced primary macrophages. Furthermore, LPS-induced activation of signaling pathways like NF-κB, p38, JNK and ERK were inhibited by Aloe vera in these cells. Altogether, we show for the first time that Aloe vera-mediated strong reduction of IL-1β appears to be the consequence of the reduced expression of both pro-IL-1β as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways. Furthermore, we show that the expression of the ATP sensor P2X7 receptor is also downregulated by Aloe vera that could also contribute to the attenuated IL-1β cytokine secretion. These results may provide a new therapeutic approach to regulate inflammasome-mediated responses.

  20. The Protective Effect of Aloe Vera on Histological Structure of Endocrine Portion of Pancreas Gland in the Diabetic Rat

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    N Erfani-Majd

    2016-01-01

    Full Text Available Introduction: Since aloe vera plant has many medical benefits, the present study aimed to investigate the protective effects of Aloe vera gel on the pancreatic islets and beta cells. Methods: This experimental study consisted of 50 mature male rats aged 2-3 months and weighed 200-250 g, who were randomly divided into five groups (n=10. Group I (control did not receive any treatments, and group II were diabetized via Streptozotocin (IP in 65 mg/kg, whose blood sample was taken after one week. Rats with blood glucose more than 250 mg/dL were considered as diabetic. Group III diabetic rats received the Aloe vera gel daily with dosage of 400 mg/kg, and group IV diabetic rats received insulin in 10 units/rat. Group V involved healthy rats which received only Aloe vera gel. After the last Aloe vera gel administration, blood glucose and body weight of all groups were measured on 15th and 30th days. Animals were euthanized with ether. Then tissues samples were collected from pancreas gland and fixed in 10% neutral buffered formalin solution. The 5-6 µ sections were made by paraffin embedding method and stained using haematoxylin-eosin (H&E and Aldehyde fuchsin stains. Ultimately, the histomorphometrical parameters were evaluated. Results: The mean number and size of pancreatic islets and beta cells of Langerhans islets decreased significantly in the diabetic group compared to the control group. The number of beta cells and diameter of langerhans islets increased significantly in the rats treated by Aloe vera gel in comparison to diabetic group at the end of 15th and 30th days. Conclusion: Applying Aloe vera gel seems to improve the renewal and restoration of langerhans islets and beta cells of pancreas gland in the diabetic rat.

  1. Gastroprotective and Anti-ulcer activity of Aloe vera juice, Papaya fruit juice and Aloe vera and Papaya fruit combined juice in Ethanol induced Ulcerated Rats

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    S. Gopinathan

    2013-12-01

    Full Text Available Peptic ulcer is the most prevalent gastrointestinal disease. Even though a wide range of drugs are available for the treatment of peptic ulcer, but many of these do not fulfill all the requirements and have side effects. These factors have attracted researchers to investigate the natural products which have more efficacy, less side effects and less expensive for the treatment of peptic ulcer disease. In the present study the anti ulcer activity of (1 Aloe vera juice, (2 papaya fruit juice (3 Aloe vera and papaya fruit combined juice were investigated in the ethanol induced ulcerated rats. The administration of plant juices decreased the offensive factors like ulcer index and acid secretion and also reduced the amount of protein and carbohydrates in the stomach fluid. Further, plant juices increased the defensive factors like activity of oxidative enzymes such as superoxide dismutase and reduced glutathione. Activities of alkaline phosphatase and lipid peroxide were higher in the diseased condition and same were reduced after the treatment with plant juices. Content of haemoglobin and RBC and WBC counts were brought back to normalcy after the treatment with plant juices. The efficacy of plant juices was comparable with the reference drug- Ranitidine. The results of the present study reveal that the plant juices are having efficiency in the gastroprotective activity. It is recommended that the above said plant juices can be further studied for their anti ulcer efficacy in human subjects.

  2. Use of dexpanthenol and aloe vera to influence the irradiation response of the oral mucous membrane (mouse); Beeinflussung der Strahlenreaktion der Mundschleimhaut (Maus) durch Dexpanthenol {+-} Aloe vera

    Energy Technology Data Exchange (ETDEWEB)

    Schlichting, S. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany); Spekl, K.; Doerr, W. [Klinik und Poliklinik fuer Strahlentherapie und Radioonkologie, Dresden Univ. (Germany)]|[Experimentelles Zentrum, Medizinische Fakultaet Carl Gustav Carus, Dresden Univ. (Germany)

    2004-07-01

    In summarising the outcome of the present study it can be said spraying the agent on the oral mucous membrane once a day had an effect on the incidence of mucous membrane ulceration in the case of both placebo and dexpanthenol treatment. However, there was no significant difference between placebo and dexpanthenol treatment, the only finding being a slight prolongation of latency time through aloe vera. These experimental findings give good reason to critically reconsider the clinical use of dexpanthenol as a supportive treatment for the prevention of radiogenic mucositis enoralis following irradiation of tumours in the head and neck region. However thorough oral lavage is an effective means of moderating the irradiation response of the oral mucous membrane. [German] Zusammenfassend ist festzustellen, dass in den vorliegenden Versuchen mit einmal taeglichem Aufspruehen des Praeparates auf die Mundschleimhaut sowohl die Placebo - wie auch die Dexpanthenol-Behandlung die Inzidenz von Schleimhautulzerationen modifiziert hat; zwischen Placebo- und Dexpanthenol-Behandlung ergab sich jedoch kein signifikanter Unterschied. Lediglich eine geringe Verlaengerung der Latenzzeit durch Aloe vera war zu beobachten. Auf der Basis dieser experimentellen Ergebnisse muss der klinische Einsatz von Dexpanthenol im Rahmen der Supportivtherapie zur Prophylaxe der radiogenen Mucositis enoralis bei der Bestrahlung von Kopf-Hals-Tumoren kritisch ueberdacht werden. Regelmaessige, intensive Mundspuelungen sind jedoch ein probates Mittel zur Verminderung der Strahlenreaktion der Mundschleimhaut. (orig.)

  3. Characterization of gelatin/chitosan scaffold blended with aloe vera and snail mucus for biomedical purpose.

    Science.gov (United States)

    López Angulo, Daniel Enrique; do Amaral Sobral, Paulo José

    2016-11-01

    Biologically active scaffolds used in tissue engineering and regenerative medicine have been generating promising results in skin replacement. The present study aims to test the hypothesis that the incorporation of Aloe vera and snail mucus into scaffolds based on gelatin and chitosan could improve their structure, composition and biodegradability, with a potential effect on bioactivity. Homogeneous pore diameter as well as pore walls in the composite scaffold could be seen in the SEM image. The pores in the scaffolds were interconnected and their sizes ranged from 93 to 296μm. The addition of Aloe vera and snail mucus enlarged the mean pore size with increased porosity and caused changes in the pore architecture. The FTIR analysis has shown good affinity and interaction between the matrix and the Aloe, which may decrease water-binding sites, so this fact hindered the water absorption capacity of the material. The mechanical properties could explain the highest swelling capacity of the snail scaffold, because the high percentage of elongation could facilitate the entry of liquid in it, generating a matrix with plenty of fluid retention. The real innovation in the present work could be the use of these substances (Aloe and snail mucus) for tissue engineering.

  4. A comparison of the leaf gel extracts of Aloe ferox and Aloe vera in the topical treatment of atopic dermatitis in Balb/c mice.

    Science.gov (United States)

    Finberg, M J; Muntingh, G L; van Rensburg, C E J

    2015-12-01

    Aloe vera gel is widely used in the treatment of an array of disturbances, especially skin disorders. The wound-healing effects have been attributed to its moisturizing and anti-inflammatory effects as well as its beneficial effect on the maturation of collagen. The aim of the present study is to compare the effects of topically applied extracts of Aloe ferox with that of Aloe vera on the symptoms as well as IgE levels of a mouse model of atopic dermatitis (AD). Mice were sensitized and challenged with 2,4-dinitrochlorobenzene and treated afterwards for 10 consecutive days with the gels of either A. ferox or A. vera applied topically to the affected areas. A placebo gel was used for the control mice. Blood was collected at the beginning and end of the treatment period to measure serum IgE levels. Although the gels of both the Aloe species inhibited the cutaneous inflammatory response as well as serum IgE levels in the rats, the extracts of A. ferox were superior to that of A. vera in reducing IgE levels. The gels of A. ferox and A. vera, applied topically, may be a safe and useful alternative to antihistamines and topical corticosteroids, for the treatment of patients suffering from recurring chronic AD.

  5. Preparation of polyamide nanocapsules of Aloe vera L. delivery with in vivo studies.

    Science.gov (United States)

    Esmaeili, Akbar; Ebrahimzadeh, Maryam

    2015-04-01

    Aloe vera is the oldest medicinal plant ever known and the most applied medicinal plant worldwide. The purpose of this study was to prepare polyamide nanocapsules containing A. vera L. by an emulsion diffusion technique with in vivo studies. Diethyletriamine (DETA) was used as the encapsulating polymer with acetone ethyl acetate and dimethyl sulfoxide (DMSO) as the organic solvents and Tween and gelatin in water as the stabilizers. Sebacoyl chloride (SC) monomer, A. vera L. extract, and olive oil were mixed with the acetone and then water containing DETA monomer was added to the solution using a magnetic stirrer. Finally, the acetone was removed under vacuum, and nanocapsules were obtained using a freeze drier. This study showed that the size of the nanocapsule depends on a variety of factors such as the ratio of polymer to oil, the concentration of polymers, and the plant extract. The first sample is without surfactant and the size of nanocapsules in the sample is 115 nm. By adding surfactant, nanocapsules size was reduced to 96 nm. Nanocapsules containing A. vera were administered to rats and the effects were compared with a normal control group. The results showed that in the A. vera group, the effect is higher. The nanocapsules were identified by scanning electron microscopy (SEM), zeta potential sizer (ZPS), and Fourier-transform infrared spectroscopy (FT-IR).

  6. UJI AKTIVITAS KOMBINASI EKSTRAK AIR LIDAH BUAYA ( Aloe vera L. DAN AKAR MANIS (Glycyrrhiza glabra L. SEBAGAI PENYUBUR RAMBUT

    Directory of Open Access Journals (Sweden)

    Sulistiorini Indriaty

    2016-05-01

    Full Text Available Aloe vera (Aloe vera L. contains amino acids, vitamins, folic acid, and liquorice (Glycyrrhiza glabra L containing glisirizin, and flavonoids, which have properties as fertilizer hair. This study aims to obtain a combination of extracts with activity most optimal fertilizer hair, made 3 combination with a combination of 1 (the water extract of liquorice concentration of 2.5% and 7.5% aloe vera, a combination of 2 (the water extract of liquorice concentrations of 2, 5% and 2.5% aloe vera, and combinations of 3 (the water extract of liquorice concentration of 5% and 7.5% aloe vera. Testing the activity of hair growth is done by measuring the rate of hair growth, hair growth acceleration and weight of rabbit hair for 28 days. Rabbit hair growth results showed the combination 1 has an average length of the largest hair with hair growth rate value of 2.97 cm, accelerated hair growth amounted to 0.15 cm / day on day 7 and weighing 0.13 gram hair.

  7. Transforming growth factor-β (TGF-β) activation in cutaneous wounds after topical application of aloe vera gel.

    Science.gov (United States)

    Takzaree, Nasrin; Hadjiakhondi, Abbas; Hassanzadeh, Gholamreza; Rouini, Mohammad Reza; Manayi, Azadeh; Zolbin, Masoumeh Majidi

    2016-12-01

    Aloe vera is a medicinal plant used to treat various skin diseases. The effects of using aloe vera gel on the healing process were investigated by microscopic methods, cell counting, and TGF-β gene expression in the wound bed. Sixty Wistar rats weighing 200-250 g were placed under anesthesia in sterile conditions. A square 1.5 cm × 1.5 cm wound was made on the back of the neck. The rats were divided into control and 2 experimental groups. Additionally, the control and experimental groups were separated into 3 subgroups corresponding to 4, 7, and 14 days of study. In the first experimental group, aloe vera was used twice on the wound. The second experimental group received aloe vera overtreatment once on the wound. The positive control group received daily application of 1% phenytoein cream following surgical wound creation. The control group did not receive any treatment. This tissue was examined using histological staining (H&E) and Masson's Trichrome. Wound surface and wound healing were evaluated separately. TGF-β gene expression was analyzed by RT-PCR. Results showed that fibroblasts in both experimental groups were significantly increased, thereby acceleration wound healing. Application of aloe vera gel will increase TGF-β gene expression, ultimately accelerating the wound healing process.

  8. Precipitation of hydroxyapatite on electrospun polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds for bone tissue engineering.

    Science.gov (United States)

    Shanmugavel, Suganya; Reddy, Venugopal Jayarama; Ramakrishna, Seeram; Lakshmi, B S; Dev, Vr Giri

    2014-07-01

    Advances in electrospun nanofibres with bioactive materials have enhanced the scope of fabricating biomimetic scaffolds for tissue engineering. The present research focuses on fabrication of polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds by electrospinning followed by hydroxyapatite deposition by calcium-phosphate dipping method for bone tissue engineering. Morphology, composition, hydrophilicity and mechanical properties of polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds along with controls polycaprolactone and polycaprolactone/aloe vera/silk fibroin nanofibrous scaffolds were examined by field emission scanning electron microscopy, Fourier transform infrared spectroscopy, contact angle and tensile tests, respectively. Adipose-derived stem cells cultured on polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds displayed highest cell proliferation, increased osteogenic markers expression (alkaline phosphatase and osteocalcin), osteogenic differentiation and increased mineralization in comparison with polycaprolactone control. The obtained results indicate that polycaprolactone/aloe vera/silk fibroin-hydroxyapatite nanofibrous scaffolds have appropriate physico-chemical and biological properties to be used as biomimetic scaffolds for bone tissue regeneration.

  9. Antitumor properties and modulation of antioxidant enzymes' activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction.

    Science.gov (United States)

    El-Shemy, H A; Aboul-Soud, M A M; Nassr-Allah, A A; Aboul-Enein, K M; Kabash, A; Yagi, A

    2010-01-01

    The aim of this study was to evaluate the potential anticancer properties and modulatory effect of selected Aloe vera (A. vera) active principles on antioxidant enzyme activities. Thus, three anthraquinones (Namely: aloesin, aloe-emodin and barbaloin) were extracted from A. vera leaves by supercritical fluid extraction and subsequently purified by high performance liquid chromatography. Additionally, the N-terminal octapeptide derived from verectin, a biologically active 14 kDa glycoprotein present in A. vera, was also tested. In vivo, active principles exhibited significant prolongation of the life span of tumor-transplanted animals in the following order: barbaloin> octapeptide> aloesin > aloe-emodin. A. vera active principles exhibited significant inhibition on Ehrlich ascite carcinoma cell (EACC) number, when compared to positive control group, in the following order: barbaloin> aloe-emodin > octapeptide > aloesin. Moreover, in trypan blue cell viability assay, active principles showed a significant concentration-dependent cytotoxicity against acute myeloid leukemia (AML) and acute lymphocytes leukemia (ALL) cancerous cells. Furthermore, in MTT cell viability test, aloe-emodin was found to be active against two human colon cancer cell lines (i.e. DLD-1 and HT2), with IC(50) values of 8.94 and 10.78 microM, respectively. Treatments of human AML leukemic cells with active principles (100 microg ml(-1)) resulted in varying intensities of internucleosomal DNA fragmentation, hallmark of cells undergoing apoptosis, in the following order: aloe-emodin> aloesin> barbaloin> octapeptide. Intererstingly, treatment of EACC tumors with active principles resulted in a significant elevation activity of key antioxidant enzymes (SOD, GST, tGPx, and LDH). Our data suggest that the tested A. vera compounds may exert their chemo-preventive effect through modulating antioxidant and detoxification enzyme activity levels, as they are one of the indicators of tumorigenesis. These

  10. Microwave-assisted fibrous decoration of mPE surface utilizing Aloe vera extract for tissue engineering applications.

    Science.gov (United States)

    Balaji, Arunpandian; Jaganathan, Saravana Kumar; Supriyanto, Eko; Muhamad, Ida Idayu; Khudzari, Ahmad Zahran Md

    2015-01-01

    Developing multifaceted, biocompatible, artificial implants for tissue engineering is a growing field of research. In recent times, several works have been reported about the utilization of biomolecules in combination with synthetic materials to achieve this process. Accordingly, in this study, the ability of an extract obtained from Aloe vera, a commonly used medicinal plant in influencing the biocompatibility of artificial material, is scrutinized using metallocene polyethylene (mPE). The process of coating dense fibrous Aloe vera extract on the surface of mPE was carried out using microwaves. Then, several physicochemical and blood compatibility characterization experiments were performed to disclose the effects of corresponding surface modification. The Fourier transform infrared spectrum showed characteristic vibrations of several active constituents available in Aloe vera and exhibited peak shifts at far infrared regions due to aloe-based mineral deposition. Meanwhile, the contact angle analysis demonstrated a drastic increase in wettability of coated samples, which confirmed the presence of active components on glazed mPE surface. Moreover, the bio-mimic structure of Aloe vera fibers and the influence of microwaves in enhancing the coating characteristics were also meticulously displayed through scanning electron microscopy micrographs and Hirox 3D images. The existence of nanoscale roughness was interpreted through high-resolution profiles obtained from atomic force microscopy. And the extent of variations in irregularities was delineated by measuring average roughness. Aloe vera-induced enrichment in the hemocompatible properties of mPE was established by carrying out in vitro tests such as activated partial thromboplastin time, prothrombin time, platelet adhesion, and hemolysis assay. In conclusion, the Aloe vera-glazed mPE substrate was inferred to attain desirable properties required for multifaceted biomedical implants.

  11. Gamma irradiation improves the antioxidant activity of Aloe vera (Aloe barbadensis miller) extracts

    Science.gov (United States)

    Mi Lee, Eun; Bai, Hyoung-Woo; Sik Lee, Seung; Hyun Hong, Sung; Cho, Jae-Young; Yeoup Chung, Byung

    2012-08-01

    Aloe has been widely used in food products, pharmaceuticals, and cosmetics because of its aromatic and therapeutic properties. In the present study, the ethanolic extracts of aloe gel were gamma-irradiated from 10 to 100 kGy. After gamma irradiation, the color of the ethanolic extracts of aloe gel changed to red; this color persisted up to 40 kGy but disappeared above 50 kGy. Liquid chromatography/mass spectrometry analysis demonstrated the production of a new, unknown compound (m/z=132) after gamma irradiation of the ethanolic extracts of aloe gel. The amount of this unknown compound increased with increasing irradiation up to 80 kGy, and it was degraded at 100 kGy. Interestingly, it was found that gamma irradiation significantly increased the antioxidant activity, as measured by the 1,1-diphenyl-2-picrylhydrazyl-radical scavenging capacity. The antioxidant activity of aloe extract was dramatically increased from 53.9% in the non-irradiated sample to 92.8% in the sample irradiated at 40 kGy. This strong antioxidant activity was retained even at 100 kGy. These results indicate that gamma irradiation of aloe extract can enhance its antioxidant activity through the formation of a new compound. Based on these results, increased antioxidant activity of aloe extracts by gamma rays can be applied to various industries, especially cosmetics, foodstuffs, and pharmaceuticals.

  12. Effects of Six Weeks Endurance Training and Aloe Vera Supplementation on COX-2 and VEGF Levels in Mice with Breast Cancer

    Science.gov (United States)

    Shirali, Saeed; Barari, Alireza; Hosseini, Seyed Ahmad; Khodadi, Elaheh

    2017-01-01

    The aim of this study was to determine effects of six weeks endurance training and Aloe Vera supplementation on COX-2 and VEGF levels in mice with breast cancer. For this purpose, 35 rats were randomly divided into 5 groups: control (healthy) and 4 cancer groups: control (cancer only), training, Aloe Vera and Aloe Vera + training. Breast cancer tumors were generated in mice by implantind. The training program comprised six weeks of swimming training accomplished in three sessions per week. Training time started with 10 minutes on the first day and increased to 60 minutes in the second week and the water flow rate was increased from 7 to 15 liters per minute at a constant rate. Aloe Vera extract at a dose of 300 mg/kg BW was administrated to rats by intraperitoneal injection. At the end of the study period, rats were anesthetized and blood samples were taken. Significant differences were concluded at pAloe Vera extract caused significant decrease in the COX-2 level in the cancer group. Also, in the training (swimming exercise) and Aloe Vera + training cancer groups, we observed significant decrease in the VEGF level as compared to controls. Our results suggest that Aloe Vera and training inhibit the COX pathway and cause decrease production of prostaglandin E2. Hence administration of Aloe Vera in combination with endurance training might synergistically improve the host milieu in mice bearing breast cancers.

  13. Efectividad del gel de aloe vera en pacientes con piodermitis subagudas

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    Arely Díaz Cifuentes

    2015-06-01

    Full Text Available Introducción: las piodermitis constituyen enfermedades cutáneas de origen bacteriano, catalogadas como enfermedades emergentes ya que se considera tienen carácter epidémico. Estas infecciones en la piel representan un motivo significativo de asistencia a las consultas dermatológicas. Su tratamiento es diverso, con antibióticos y crema de uso tópico, pero también puede ser utilizada el aloe vera en las lesiones, fundamentalmente por el poder emoliente suavizante que ejerce. Objetivo: determinar la efectividad del gel de aloe vera (Sábila en las piodermitis sub agudas, en pacientes que acudieron a la consulta de dermatología del consultorio “El Golfito” en el municipio de Cabimas, Venezuela, en el periodo comprendido entre 1 de enero hasta el 31 de diciembre de 2013. Métodos: se realizó un estudio experimental, exploratorio, correlacional, para determinar la efectividad del gel de aloe vera en las piodermitis sub agudas, en los pacientes, lugar y periodo de tiempo declarados. El universo de estudio estuvo constituido por 150 pacientes que asistieron a la referida consulta, diagnosticados clínicamente con esta entidad; la muestra quedó conformada por 50 pacientes, de ambos sexos y menores de 70 años. Se indicó tratamiento con antibiótico oral, amoxicillina en dosis de 50mg/kg al día dividida en tres dosis (cada 8 horas en niños hasta 15 años, a partir de esta edad la dosis fue de 500 mg cada 8 horas por 7 días y el uso tópico del gel del aloe en las lesiones tres veces al día hasta lograr la curación. Resultados: el grupo de edad más afectado estuvo en los menores de diecinueve años (42%; en las condiciones socioeconómicas de las familias e higiénico sanitaria de las viviendas predominaron las evaluadas de regular (74% - 70%. Las lesiones elementales más frecuentes al inicio fueron las exulceraciones (86%, mientras que a los 7 y 15 días predominan las máculas (82% - 96%. Se logra la curación en el 82% de los

  14. Case Reports of Bedsores Using Aloe Vera Gel Powder with High Molecular Weight

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    Keizo Matsuo

    2009-01-01

    Full Text Available It has been reported that the Japanese have higher rate of life expectancy and that Japan is rapidly becoming a super aging society. Almost 5 per cent of the aged, who develop diseases such as cerebrovascular related disorders, mental problems, fractures, cancer and infections also end up suffering from bedsores. It has been found that patients often opt for treatment, when the bedsores become very severe and adding more complications in their treatment. It may be presumed that prevention and early detection are vital for the treatment of bed sores. In our present study, we have tested herbal formulations having Aloe vera gel powder for its efficacy and activity on bed sores. Aloe vera gel powder with high molecular weight (AHM was prepared from the gel part, by washing with running water using the patented freeze-drying under micro wave and far infra red irradiations in which barbaloin content was less than 10 ppm in powder form. The treatment was given by applying the macromolecule aloe ointment for bedsores from I degree to Il degree ulcer patients. The results have shown that AHM in the ointment form indicated a high possibility to cure bedsores. Being very difficult to cure, due to the patient′s peculiar conditions such as old age, inability of the patient to turn by himself/herself and also due to complications caused by other symptoms. We were able to confirm the effectiveness of the macromolecule aloe ointment in four cases of bedsores with two cases of positive control, using the Design Score and by checking the side effects. In this study, our report is based on the preclinical trials for bedsores by the external use of the macromolecule aloe ointment.

  15. QUALITATIVE AND QUANTITATIVE PROFILE OF ALOIN ISOLATED FROM ALOE VERA

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    Soni Himesh

    2011-09-01

    Full Text Available A.vera is known to contain well over 100 separate ingredients or constituents between those found in the leaf and those found in the mucilaginous gel inside the leaf. It is also known that some of the ingredients found in the leaf such as Aloin, or the Emodins are recognized as having laxative and antimicrobial properties. Aloin is Folk Traditional Medicine used for Healing of minor cutaneous injuries, such a blisters, abrasions, cuts, burns, bites and scares, protection and care of external skin, such as cleansing, moisturizing, tightening and in mixes as sunscreens and anti-chapping compounds. In the present work, we have investigated the qualitative and quantitative determination of Aloin isolated from the A.vera. Qualitative estimation was carried out by treating the sample with bromine water and thin layer chromatographic (TLC method. The simultaneous determination of the Aloin was carried out by HPLC technique. HPLC separation was performed on a Cyber Lab C-18 column (250 x 4.0 mm, 5μ using methanol (A and 0.34% acetic acid (B using a isocratic elution as follow: 0–30 min, 40%A–80% A, 60%B–20% B. The flow rate was 1.0 mL/min, and a column temperature of 25°C. The injection volume was 25µl, and UV detection was effected at 297.5 nm.

  16. An in vitro and in vivo toxicologic evaluation of a stabilized aloe vera gel supplement drink in mice.

    Science.gov (United States)

    Sehgal, Inder; Winters, Wallace D; Scott, Michael; Kousoulas, Konstantine

    2013-05-01

    Aloe vera gel is increasingly consumed as a beverage dietary supplement. The purpose of this study was to determine potential toxicity of a stabilized aloe vera gel derived from the inner gel fillet and marketed as a drink. The gel juice was assessed through assays of genotoxicity in vivo and acute and subchronic toxicity in B6C3F1 mice. Aloe vera did not increase the SOS DNA repair response in Escherichia coli and at 1× and 0.25× it did not increase mutagenesis of Salmonella TA100 resulting in histidine biosynthesis. At 3 and 14days following acute exposure, male and female mice gavaged with the stabilized aloe gel had daily appearances, total body weight gain, selected organ weights, necropsy and hematology tests similar to control mice gavaged with water. After a 13-week aloe gel feed study, male and female mice evaluated by the same criteria as the acute study plus feed consumption and serum chemistry tests were found to be equivalent to control groups. These data indicate that a commercial stabilized aloe gel consumed as a beverage was not genotoxic or toxic in vivo. These results contrast with those obtained using preparations containing aloe latex phenolic compounds such as anthraquinones.

  17. A comparative study of baby immature and adult shoots of Aloe vera on UVB-induced skin photoaging in vitro.

    Science.gov (United States)

    Hwang, Eunson; Kim, Su Hyeon; Lee, Sarah; Lee, Choong Hwan; Do, Seon-Gil; Kim, Jinwan; Kim, Sun Yeou

    2013-12-01

    Ultraviolet (UV) irradiation induces photo-damage of the skin, which in turn causes depletion of the dermal extracellular matrix and chronic alterations in skin structure. Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase (MMP) expression. The production of type I procollagen is regulated by transforming growth factor-β1 (TGF-β1) expression; the activation of MMP is also correlated with an increase of interleukin-6 (IL-6). Aloe barbadensis M. (Aloe vera) is widely used in cosmetic and pharmaceutical products. In this study, we examined whether baby aloe shoot extract (BAE, immature aloe extract), which is from the one-month-old shoots of Aloe vera, and adult aloe shoot extract (AE), which is from the four-month-old shoots of Aloe vera, have a protective effect on UVB-induced skin photoaging in normal human dermal fibroblasts (NHDFs). The effects of BAE and AE on UVB-induced photoaging were tested by measuring the levels of reactive oxygen species, MMP-1, MMP-3, IL-6, type I procollagen, and TGF-β1 after UVB irradiation. We found that NHDF cells treated with BAE after UVB-irradiation suppressed MMP-1, MMP-3, and IL-6 levels compared to the AE-treated cells. Furthermore, BAE treatment elevated type I procollagen and TGF-β1 levels. Our results suggest that BAE may potentially protect the skin from UVB-induced damage more than AE.

  18. Dietary Aloe vera supplementation on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT).

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; He, Jie; Ma, Xin Yu; Kpundeh, Mathew D; Xu, Pao

    2015-06-01

    This study investigated effects of dietary Aloe vera on growth performance, some haemato-biochemical parameters and disease resistance against Streptococcus iniae in tilapia (GIFT). Five groups were designed including a basal diet (control) and 100% A. vera powder incorporated in fish feed at 0.5% 1%, 2%, and 4%/kg feed, which were administered for 8 weeks. Fish fed 0.5%, 1%, and 2% A. vera supplemented diet significantly improved (p vera diet at 1% and 2%/kg feed. Feed efficiency ratio, feed conversion ratio, and hepatosomatic index were significantly enhanced in 4% A. vera supplemented fish over unsupplemented ones (p vera supplemented fish showed a significant increase (p vera supplemented fish showed a decrease (p vera diet at 2% and 4% A. vera/kg feed than those fed unsupplemented diet. Unchallenged fish fed 0.5%, 1%, and 2% A. vera showed significantly higher values (p vera supplemented diet. There was a significant increase (p vera unsupplemented fish and those supplemented with A. vera diet at 1%/kg feed increased significantly (p vera supplemented diet maintained higher values at all experimental stages among groups. There was a significant correlation (p Aloe had no significant effect (p > 0.05) on the survival of the fish when compared to the control; no mortality was recorded in challenge trial. Overall, our results indicated that dietary aloe supplementation could improve growth, feed utilization, and haemato-biochemical parameters of cultured tilapia.

  19. Aloe vera extract as a promising treatment for the quality maintenance of minimally-processed table grapes

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    Giuseppina Rosaria Antonella Alberio

    2015-06-01

    Full Text Available The effect of an edible film obtained from a commercial Aloe vera extract, on the quality maintenance of minimally processed grapes belonging to three different cultivars (Sugar One, Victoria and Black Magic was evaluated by enzymatic (PPO, PME, β-GAL, physicochemical (pH, acidity, °Brix, and sensorial methods. All the analyzed parameters were measured in extracts obtained from minimally processed grapes packaged in ordinary atmosphere and stored at 4 °C for 15 days. Samples dipped into Aloe vera showed significant differences (p≤0.05 compared to untreated ones. The determination of such parameters and the evaluation of consumer acceptability were helpful to determine the effectiveness of the post-harvest treatment with Aloe vera for a storage period of 15 days.

  20. Enhanced biocompatibility of ZnS:Mn quantum dots encapsulated with Aloe vera extract for therapeutic applications

    Science.gov (United States)

    Anilkumar, M.; Bindu, K. R.; Sneha Saj, A.; Anila, E. I.

    2016-08-01

    Toxicity of nanoparticles remains to be a major issue in their application to the biomedical field. Aloe vera (AV) is one of the most widely exploited medicinal plants that have a multitude of amazing properties in the field of medicine. Methanol extract of Aloe vera can be used as a novel stabilising agent for quantum dots to reduce toxicity. We report the synthesis, structural characterization, antibacterial activity and cytotoxicity studies of ZnS:Mn quantum dots synthesized by the colloidal precipitation method, using methanol extract of Aloe vera (AVME) as the capping agent. The ZnS:Mn quantum dots capped with AVME exhibit superior performances in biocompatibility and antibacterial activity compared with ZnS:Mn quantum dots without encapsulation. Project supported by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India.

  1. Effect of a dentifrice containing aloe vera on plaque and gingivitis control: a double-blind clinical study in humans

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    Sílvia Morgana Araújo de Oliveira

    2008-08-01

    Full Text Available The effect of Aloe vera on the reduction of plaque and gingivitis was evaluated in a randomized, parallel and double-blind clinical trial. Subjects were randomly allocated to the test group (n=15 - dentifrice containing Aloe vera - or the control group (n=15 - fluoridated dentifrice. Plaque index (PI and gingival bleeding index (GBI were assessed at days 0 and 30. Subjects were asked to brush their teeth with the control or test dentifrice, three times a day, during a 30-day period. There was a significant reduction on plaque and gingivitis in both groups, but no statistically significant difference was observed among them (p>0.01. The dentifrice containing Aloe vera did not show any additional effect on plaque and gingivitis control compared to the fluoridated dentifrice.

  2. In vitro assessment of the prebiotic potential of Aloe vera mucilage and its impact on the human microbiota.

    Science.gov (United States)

    Gullón, Beatriz; Gullón, Patricia; Tavaria, Freni; Alonso, José Luis; Pintado, Manuela

    2015-02-01

    Aloe vera mucilage is reported to be rich in acemannan that is a polysaccharide with a backbone of β-(1→4)-D-mannose residues acetylated at the C-2 and C-3 positions and contains some side chains of galactose and arabinose attached to the C-6 carbon. The evaluation of the prebiotic potential of Aloe vera mucilage was carried out by in vitro fermentation using intestinal microbiota from six healthy donors as the inoculum. The prebiotic activity was assessed through the quantification of short chain fatty acids (SCFA) and the evaluation of dynamic bacterial population in mixed faecal cultures by fluorescence in situ hybridization (FISH). Our findings support the possible incorporation of the Aloe vera mucilage in the development of a variety of food products known as prebiotics aimed at improving gastrointestinal health.

  3. Salinity stress effects changed during Aloe vera L. vegetative growth

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    Jamal-Ali Olfati

    2012-05-01

    Full Text Available Salt stress is a limiting factor of plant growth and yield, and becoming a serious problem in the world so in reason to determine salinity effect on aloe growth experiment was conducted in a greenhouse as a bi-factorial in completely randomized experimental design with three replications. Aloe plants irrigated with nutrient solution containing different level of NaCl (0, 2, 4, 6 and 8 ms on December and harvesting took place three time with one month interval on January, February and March. Results revealed that salinity affected number of leaf, plant height, number of sprout, root weight, plant weight, leaf weight, total gel weight, and root dry weight. Variance analysis also showed that sampling time relieves significant effect on number of leaf, plant height, and root length, number of sprout, root weight, plant weight, leaf weight and total gel weight. All measured characteristics were highest for control. Interaction between salinity and sampling time on plant height, plant weight, leaf weight and total gel weight showed that these traits decreased in all salinity levels when sampled 30 days after transplanting and the lowest value was related to highest salinity. This result was similar 60 and 90 days after transplanting. Interaction between salinity and sampling time on root length showed that the highest root length was obtained in 2 and 4 ms salinity level when sampled 30 days after transplanting. However all salinity levels also decreased root length 90 days after transplanting.

  4. The Anti-Nociceptive Effect of Aloe. Vera Aqueous Extract in Fructose-Fed Male Rats

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    Mohammad Reza Shahraki

    2010-05-01

    Full Text Available A B S T R A C T Introduction: Aloe Vera extract is used as an anti-inflammatory and anti-bradikinin agent in laboratory animals. The aim of this survey was to evaluate the ant-nociceptive effect of A. Vera aqueous extract in fructose-fed male rats. Methods: Forty-five Wistar-Albino male rats were equally and randomly divided into five groups including sham operated and four test groups. Sham operated group consumed tap water and the test groups consumed fructoseenriched water. Test groups 2, 3 and 4 additionally received, 0, 100, 150 and 200 mg/kg of A. Vera extract, respectively, whereas the other test group received distilled water daily. Tail flick reaction time, serum glucose and oral glucose tolerance test (OGTT were measured. The results were analyzed by SPSS software using ANOVA and Tukey tests. Results were expressed as mean ± SD. Statistical differences were considered significant at p<0.05. Results: The results showed that tail flick reaction time significantly increased in test group 3 which received 200 mg/kg A. Vera extract comparing with that of sham operated group. However, OGTT and serum glucose value were significantly increased in all fructose-fed male rats comparing with those of sham operated group. Discussion: These results indicated that A. Vera aqueous extract can affect tail flick reaction time in fructose-fed male rats. Further studies are required to show the exact mechanism of anti-nociceptive effect of A. Vera extract.

  5. Anticancer activity of Aloe vera and Calligonum comosum extracts separetely on hepatocellular carcinoma cells

    Institute of Scientific and Technical Information of China (English)

    Maram; Shalabi; Kh.Khilo; Mahmoud; M.Zakaria; Mahmoud; G.Elsebaei; Walied; Abdo; Walaa; Awadin

    2015-01-01

    Objective: To investigate the in vitro anticancer effect of Aloe vera(A. vera) and Calligonum comosum(C. comosum) extracts against hepatocellular carcinoma(HepG 2) cells. Methods: Hep G2 cells were tested against different doses of A. vera and C. comosum. Viability of the cells was assessed by MTT assay. Evaluation of apoptosis and DNA damage in HepG 2 cells were performed using annexin V apoptosis detection kit. The expression of p53 and anti-apoptotic(Bcl-2) were tested by real time-PCR and flow cytometer analyser. Hematoxylin and eosin stained sections from untreated and treated HepG 2 cells were observed using light microscopy.Results: The IC50 values of A. vera and C. comosum extracts were(10.45 ± 0.31) and(9.60 ± 0.01) μg/mL respectively. The extracts separately increased cytotoxicity against HepG 2 cells in a time and dose dependent manners. Also, it apparently induced apoptosis through increase P53 and decrease Bcl-2 genes expressions.Conclusions: The results indicated that the extracts could have anti-hepatocarcinogenic effect, at least in part, through modulation of apoptosis.

  6. Propriedades farmacológicas da Aloe vera (L. Burm. f.

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    V.S. Freitas

    2014-06-01

    Full Text Available A Aloe vera (L. Burm.f. tem sido utilizada há milhares de anos na medicina tradicional para o tratamento de diversos males. O intuito desse trabalho foi o levantamento bibliográfico de artigos que evidenciassem a atividade farmacológica da Aloe vera. A revisão contemplou livros e periódicos nacionais e internacionais indexados nas bases de dados MEDLINE, LILACS e SciElo, nos idiomas português, inglês e espanhol, utilizando as palavras-chave citadas. Após o levantamento bibliográfico, constatou-se que várias atividades biológicas são atribuídas a Aloe vera. Evidências sugerem eficácia no tratamento da psoríase, herpes genital, queimaduras e hiperglicemia. Além disto, também foram demonstradas atividades antineoplásica, antimicrobiana, anti-inflamatória e imunomodulatória por estudos in vitro e in vivo, entretanto, na cicatrização de feridas, os resultados foram conflitantes. No tratamento de dermatite por radiação e em queimaduras solares sua eficácia não foi comprovada e foram relatados casos de hepatite aguda devido ao consumo de preparações orais. Tendo em vista as várias atividades comprovadas e poucos relatos acerca de sua contra indicação, conclui-se que o uso desta espécie corrobora o vasto uso popular.

  7. Aloe vera inhibits proliferation of human breast and cervical cancer cells and acts synergistically with cisplatin.

    Science.gov (United States)

    Hussain, Arif; Sharma, Chhavi; Khan, Saniyah; Shah, Kruti; Haque, Shafiul

    2015-01-01

    Many of the anti-cancer agents currently used have an origin in natural sources including plants. Aloe vera is one such plant being studied extensively for its diverse health benefits, including cancer prevention. In this study, the cytotoxic potential of Aloe vera crude extract (ACE) alone or in combination with cisplatin in human breast (MCF-7) and cervical (HeLa) cancer cells was studied by cell viability assay, nuclear morphological examination and cell cycle analysis. Effects were correlated with modulation of expression of genes involved in cell cycle regulation, apoptosis and drug metabolism by RT-PCR. Exposure of cells to ACE resulted in considerable loss of cell viability in a dose- and time-dependent fashion, which was found to be mediated by through the apoptotic pathway as evidenced by changes in the nuclear morphology and the distribution of cells in the different phases of the cell cycle. Interestingly, ACE did not have any significant cytotoxicity towards normal cells, thus placing it in the category of safe chemopreventive agent. Further, the effects were correlated with the downregulation of cyclin D1, CYP 1A1, CYP 1A2 and increased expression of bax and p21 in MCF-7 and HeLa cells. In addition, low dose combination of ACE and cisplatin showed a combination index less than 1, indicating synergistic growth inhibition compared to the agents applied individually. In conclusion, these results signify that Aloe vera may be an effective anti-neoplastic agent to inhibit cancer cell growth and increase the therapeutic efficacy of conventional drugs like cispolatin. Thus promoting the development of plant-derived therapeutic agents appears warranted for novel cancer treatment strategies.

  8. The effect of aloe vera bioactive level as feed additive on the egg performances of laying hens

    OpenAIRE

    I.A.K Bintang; A.P Sinurat; T Purwadaria

    2005-01-01

    A study on the use of aloe vera bioactives as feed additive in layer ration was conducted. One hundred and twenty pullets strain Isa Brown were allocated into 5 treatments with 6 replicates and 4 birds/replicate. The treatments were: control,control+antibiotic (50 ppm zinc bacitracin), and control+ aloe vera at three levels (0.25; 0.50 and 1.00 g/kg). The treatments were conducted in a completely randomized design. Parameter measured were first initial body weight, age at 1st lay, feed intake...

  9. Effects of different levels of Aloe vera gel as an alternative to antibiotic on performance and ileum morphology in broilers

    OpenAIRE

    Ali Mahdavi; Ahmad Zare Shahneh; Abolfazl Zarei; Babak Darabighane

    2011-01-01

    The present study attempts to analyze the effects of different levels of Aloe vera gel as an alternative to antibiotic, on performance and ileum morphology in broilers. Three hundred one-day old Ross 308 male broilers were used on a completely randomized design in 5 groups with 4 replicates, each consisting of 15 broilers. The groups included the control group (basal diet) and three groups with basal diet mixed with different levels of Aloe vera gel (1.5%, 2% and 2.5%). Finally, there was a g...

  10. Semen characteristics and sperm morphological studies of the West African Dwarf Buck treated with Aloe vera gel extract

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    Oyeyemi Matthew Olugbenga

    2011-01-01

    Full Text Available Background: Aloe vera (Aloe barbadensis miller is an evergreen perennial plant widely used in modern herbal practice and is often available in proprietary herbal preparations.Objective: This study was designed to investigate the semen picture and spermatozoa morphology of West African Dwarf (WAD bucks treated with Aloe vera extract.Materials and Methods: Twelve sexually matured WAD bucks, weighing between 11 and 15 kg, were used for the study. The bucks were first used as control (pre-treatment and later as two groups of six animals each. The first six bucks received 10 mls of the 3% extract while the other six received 10 mls of the 4% of the extract for a 7 day period. Semen was collected from both the 3 and 4% extract treated bucks for the control (pre-treatment, on days eight (first week post-treatment and fifteen (second week post-treatment in each case using the electroejaculation method. The spermiogram of the bucks were investigated using standard procedures. Data obtained were analyzed using two way ANOVA and significance reported at p<0.05.Results: The continuous administration of Aloe vera extract significantly (p<0.05 reduced sperm concentration, motility and percentage livability and resulted in increased sperm abnormalities in the WAD buck.Conclusion: Aloe vera adversely affected the spermiogram of bucks. The plant can reduce fertility in male animals and is therefore not recommended for medicinal purpose in male animals especially those used for breeding.

  11. Histological study of wound repair with topical aloe vera gel in rat

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    Nasrin Takzaree

    2015-12-01

    Full Text Available Background: One of the most important issues in medical sciences is wound healing and repair. Application of natural ingredients and herbs for treating ulcers has been in the history of human life. Nowadays, due to the lack of side effects of medicinal plants and a variety of effective compounds in plants, as well as numerous disadvantages of synthetic drugs there has been tendency to use medicinal plants in clinic. Aloe vera is an herbal drug used for treatment of dermal diseases. In this study we evaluated, effects of aloe vera on the wound healing through the microscopic techniques and cell counting. Methods: In this experimental study, sixty Wistar rats weighing 200-250 g were placed under general anesthesia and sterile conditions. Then a square shape wound with 1.5´1.5 mm dimension was made on the back of the neck. Rats were randomly divided to control and experimental group’s. Each group was divided to three subgroups with 4, 7, and 14 study days. In 1st experimental group aloe vera was used twice on the wound surface and in 2nd experimental group was used once daily and the positive control group were applied phenytoin cream 1% from the zero days of surgery. The control group did not get any treatment on the wound surface. For histological studies, during the fourth, seventh and fourteenth day’s rats were sacrificed and samples were taken from the wound area and adjacent skin. After histological staining with hematoxylin and eosin (H&E and Masson's trichrome stains, the cells were counted, wound surface and wound healing were investigated. Results: The macroscopic and microscopic evaluation showed that wound healing increased because the fibroblast numbers in two experimental groups improved compared with control group. The percentage of wound healing on different days in the experimental and control groups were significant. Data were analyzed by using one-way ANOVA test and P< 0.05 was significant. Conclusion: Present study showed

  12. The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers

    OpenAIRE

    Tiurma Pasaribu; A.P Sinurat; T Purwadaria

    2006-01-01

    A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG) as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD), egg weight, feed consumption, feed conversion ratio (FCR), egg quality...

  13. A Randomised, Cross-Over, Placebo-Controlled Study of Aloe vera in Patients with Irritable Bowel Syndrome: Effects on Patient Quality of Life.

    Science.gov (United States)

    Hutchings, H A; Wareham, K; Baxter, J N; Atherton, P; Kingham, J G C; Duane, P; Thomas, L; Thomas, M; Ch'ng, C L; Williams, J G

    2011-01-01

    Background. Irritable bowel syndrome (IBS) is a chronic, difficult to treat condition. The efficacy of Aloe vera in treating IBS symptoms is not yet proven. The purpose of this study was to determine if Aloe vera is effective in improving quality of life. Methods. A multicentre, randomised, double-blind, cross-over placebo controlled study design. Patients were randomised to Aloe vera, wash-out, placebo or placebo, washout, Aloe vera. Each preparation (60 mL) was taken orally twice a day. Patient quality of life was measured using the Gastrointestinal Symptoms Rating Score, Irritable Bowel Syndrome Quality of Life, EuroQol and the Short-Form-12 at baseline and treatment periods 1 and 2. Results. A total of 110 patients were randomised, but only 47 completed all questionnaires and both study arms. Statistical analysis showed no difference between the placebo and Aloe vera treatment in quality of life. Discussion. This study was unable to show that Aloe vera was superior to placebo in improving quality of life. Drop outs and other confounding factors may have impacted on the power of the study to detect a clinically important difference. Conclusion. This study failed to find Aloe vera superior to placebo in improving quality of life proven Irritable Bowel Syndrome patients.

  14. El uso terapéutico del Aloe Vera en las Úlceras Por Presión (UPP

    Directory of Open Access Journals (Sweden)

    Francisco José Hernández Martínez

    2010-01-01

    Full Text Available El Aloe Vera es un excelente limpiador y antiséptico natural que penetra fácilmente en la piel y tejidos, con gran actividad bactericida, fungicida, antiinflamatorio, antiprurítico, dilata los capilares sanguíneos, descompone y destruye los tejidos muertos y favorece el crecimiento celular. El objetivo de este trabajo fue averiguar si el uso de Aloe Vera en UPP es eficaz como tratamiento. Estudio cualitativo de 59 pacientes con UPP ingresados en un Hospital Geriátrico, realizando un protocolo de curas con una cataplasma de Aloe Vera triturado. La edad media fue de 76.2% años. Distribución de la muestra: 13 pacientes con UPP Grado I, 21 Grado II ,15 Grado III y 10 Grado IV.El 99% de grado I evolucionaron fenomenalmente al tratamiento con Aloe, todas desaparecieron entre 3 y 7 días. El 1% presentó una alergia al Aloe triturado.UPP grado II, aparece mejoría a las 24 horas y todas curaron entre 12-14 días con una ligera variación del color de la piel. Grado III y IV evolucionaron de forma diferente a las anteriores. El 5% grado III terminaron curándose, 95% restante sufrieron modificaciones del tejido afectado; disminuyendo el exudado y favoreciendo la coloración. A partir de 4 ó 5 días la musculatura y tejidos implicados en la UPP, variaban su coloración y el lecho se secaba. En el 98% de Grado IV aparecía enrojecimiento y quemaduras después de aplicar Aloe. El Aloe Vera puede ayudar a curar UPP Grado I y II. Dudosa evidencia científica para uso, en UPP Grado III y IV. Un porcentaje pequeño ha presentado reacciones adversas de tipo alérgico.

  15. Quality and Acceptability of Meat Nuggets with Fresh Aloe vera Gel.

    Science.gov (United States)

    Rajkumar, V; Verma, Arun K; Patra, G; Pradhan, S; Biswas, S; Chauhan, P; Das, Arun K

    2016-05-01

    Aloe vera has been used worldwide for pharmaceutical, food, and cosmetic industries due to its wide biological activities. However, quality improvement of low fat meat products and their acceptability with added Aloe vera gel (AVG) is scanty. The aim of this study was to explore the feasibility of using fresh AVG on physicochemical, textural, sensory and nutritive qualities of goat meat nuggets. The products were prepared with 0%, 2.5%, and 5% fresh AVG replacing goat meat and were analyzed for proximate composition, physicochemical and textural properties, fatty acid profile and sensory parameters. Changes in lipid oxidation and microbial growth of nuggets were also evaluated over 9 days of refrigerated storage. The results showed that AVG significantly (p<0.05) decreased the pH value and protein content of meat emulsion and nuggets. Product yield was affected at 5% level of gel. Addition of AVG in the formulation significantly affected the values of texture profile analysis. The AVG reduced the lipid oxidation and microbial growth in nuggets during storage. Sensory panelists preferred nuggets with 2.5% AVG over nuggets with 5% AVG. Therefore, AVG up to 2.5% level could be used for quality improvement in goat meat nuggets without affecting its sensorial, textural and nutritive values.

  16. The effectiveness of Aloe vera barbadensis bioactives on laying hens on commercial farmers

    Directory of Open Access Journals (Sweden)

    Tiurma Pasaribu

    2006-06-01

    Full Text Available A field trial was conducted to study the effectiveness of dry gel Aloe vera (DG as a feed additive for laying hens in commercial farms. The trial was consisted of two treatments, one was control, commonly used farmer ration containing antibiotic of zinc bacitracin at 0.5 g/kg and the second feed containing DG equal to 1.0 g/kg. Every treatment had two replicates with 504 Loghman laying hens. The hen day production (% HD, egg weight, feed consumption, feed conversion ratio (FCR, egg quality comprising yolk colour, albumin and yolk weights, egg shell eight and thikness, and mortality were observed for 24 weeks. The results showed that feed consumption, % HD, egg weight, FCR, yolk colour, albumin weight, yolk weight, egg shell weight, and egg shell thickness were not significantly different (P>0.05 between the control and DG treatment, except for the Haugh unit (HU. Thus, it can be concluded that Aloe vera bioactives has the same effectiveness as antibiotic as a feed additive at the level of commercial farms.

  17. A randomized, double‑blind clinical study to assess the antiplaque and antigingivitis efficacy of Aloe vera mouth rinse

    Directory of Open Access Journals (Sweden)

    Bathini Chandrahas

    2012-01-01

    Full Text Available Aims: To evaluate the efficacy of Aloe Vera mouth rinse on experimental plaque accumulation and gingivitis. Materials and Methods: In this randomized, controlled, and double-blind study, a total of 148 systemically healthy subjects were screened in the age group of 18-25 years. Finally, 120 subjects were requested to abstain from oral hygiene (tooth brushing for 14 days and used a specially fabricated plaque guard. Following cessation of tooth brushing in the specified area, the subjects were randomly divided into Group A (test group who received 100% Aloe vera, Group B (negative control group who received placebo (distilled water, and Group C (positive control group who received 0.2% chlorhexidine. The rinse regimen began on the 15 th day and continued for 7 days. Plaque accumulation was assessed by Plaque Index (PI and gingivitis was assessed by Modified Gingival Index (MGI and Bleeding Index (BI at baseline (0, 7 th , 14 th , and 22 nd days. Results: There was statistically significant decrease in PI, MGI, and BI scores after the rinse regimen began in both Group A (test group and Group C (chlorhexidine compared with Group B. Mouth wash containing Aloe vera showed significant reduction of plaque and gingivitis but when compared with chlorhexidine the effect was less significant. Conclusion: Aloe vera mouthwash can be an effective antiplaque agent and with appropriate refinements in taste and shelf life can be an affordable herbal substitute for chlorhexidine.

  18. Mosquitocidal and water purification properties of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus leaf extracts.

    Science.gov (United States)

    Ethanolic extracts of Cynodon dactylon, Aloe vera, Hemidesmus indicus and Coleus amboinicus were tested for toxicity to 3rd instar Anopheles stephensi, Culex quinquefasciatus, and Aedes aegypti. Median lethal concentrations (LC50) were, respectively, 0.44%, 0.51%, 0.59% and 0.68%. Cynodon dactylon...

  19. Clinical and radiographic evaluation of zinc oxide with aloe vera as an obturating material in pulpectomy: An in vivo study

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    Abhishek Khairwa

    2014-01-01

    Full Text Available Background: Pulp therapy for pulpally involved primary teeth continues to be a challenge to clinicians. One of the major areas of continued research is in the area of finding obturating materials to suit the specific properties of these teeth. Zinc oxide eugenol is used frequently in pulpectomy for the obturation of the primary teeth. Aims: To evaluate clinically and radiographically a mixture of zinc oxide eugenol and aloe vera as an obturating material. Materials and Methods: A total of 50 children, aged between 4 and 9 years, who were screened for unilateral or bilateral carious deciduous molars were studied. Out of these, 15 children were randomly selected for endodontic treatment. Obturation was done with a mixture of zinc oxide powder and aloe vera gel. Clinical and radiographic evaluation was done after 7 days, 1 month, 3 months, 6 months, and 9 months. The data were statistically analyzed. Results and Conclusion: Endodontic treatment using a mixture of zinc oxide powder and aloe vera gel in primary teeth has shown good clinical and radiographic success. A detailed observational study with longer follow-up will highlight the benefits of aloe vera in primary teeth as an obturating medium.

  20. Effects of the application of Aloe vera (L. and microcurrent on the healing of wounds surgically induced in Wistar rats Efeitos da aplicação de Aloe vera (L. e microcorrente no reparo de lesões cirúrgicas induzidas em ratos Wistar

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    Cristina Cruz Franchini

    2009-04-01

    Full Text Available PURPOSE: To investigate the effects of topical application of an Aloe vera gel combined or not with microcurrent application on the healing of skin wounds surgically induced in Wistar rats. METHODS: The animals were randomly divided into the following groups: control group, animals topically treated with Aloe vera, animals treated with a microcurrent, and animals receiving topical application of Aloe vera combined with microcurrent application. RESULTS: The results indicated differences in wound healing between the various treatments when compared to the control group. Tissue hyperplasia was lower in the control group compared to the other treated groups. Accelerated wound healing was observed in the group treated with Aloe vera compared to control. Animals submitted to microcurrent application only and the group treated with microcurrent plus Aloe vera presented an earlier onset of the proliferative phase compared to the control group and animals treated with Aloe vera gel alone. Morphometric data confirmed the structural findings. CONCLUSION: Simultaneous application of Aloe vera gel and microcurrent is an excellent choice for the treatment of open wounds thus indicating a synergistic action of these two applications.OBJETIVO: Investigar os efeitos da aplicação tópica do gel de Aloe vera, combinada ou não com a aplicação de microcorrente no reparo de lesões cutâneas induzidas cirurgicamente em ratos Wistar. MÉTODOS: Os animais foram distribuídos aleatoriamente em: grupo controle, tratado topicamente com gel in natura de Aloe vera, tratado com microcorrente e tratado com aplicação tópica de Aloe vera associada à microcorrente. RESULTADOS: Os resultados do presente trabalho indicaram que o reparo tecidual ocorreu de forma diferenciada nos vários tratamentos empregados quando comparados ao grupo controle. A hiperplasia tecidual no grupo controle foi menor que a observada nos demais grupos tratados. No grupo tratado com aplica

  1. Comparative evaluation of Green Tea- Aloe Vera mouthwash and chlorhexidine 0.2% on gingival indices (A randomized clinical trial

    Directory of Open Access Journals (Sweden)

    Naser Sargolzaie

    2015-12-01

    Full Text Available Introduction: Nowadays in the field of dentistry, there is a trend to encourage the use of herbal and natural products. The high level of clinical research in terms of considering green tea and aloe vera, with various functionality for individual use is a typical example. The purpose of this randomized, controlled, and double-blind study was to evaluate comparative evaluation of green tea-aloe vera mouthwash and chlorhexidine 0.2% on gingival indices. Methods: A total of 60 patients (26 women and 34 men with periodontal disease were randomly allocated into one of the three double blind groups, 20 in each, to receive the following treatments :(1 0.2% chlorhexidine, (2 green tea-aloe vera and  (3 distilled water. Plaque and gingival indices were evaluated on the day of the beginning of the experiment and 14 days postoperative. Subjects were asked to rinse their mouth with the mouth rinse, twice a day, during a 14-day period. Paired t-test was used to test the mean difference on 0 and 14 days, respectively. One way analysis of variance (ANOVA was used to compare the mean difference between the groups while Tukey test was used for multiple comparisons. Results: Chlorhexidine 0.2%, green tea - aloe vera and placebo reduced the plaque index by 0.17±0.14, 0.10±0/08 and 0.02±0.18, respectively, with a statistical significance of p=0.008. This difference was related to Chlorhexidine 0.2% and green tea - aloe vera with Placebo. There exist a significant difference of p=0.001 in the bleeding index  between the three groups, and this difference was related to Chlorhexidine 0.2% and green tea - aloe vera with placebo. Conclusion: Green tea –aloe vera   mouthwash improves periodontal health status. Therefore, it can be used to improve oral and dental health status.

  2. Clear evidence of carcinogenic activity by a whole-leaf extract of Aloe barbadensis miller (aloe vera) in F344/N rats.

    Science.gov (United States)

    Boudreau, Mary D; Mellick, Paul W; Olson, Greg R; Felton, Robert P; Thorn, Brett T; Beland, Frederick A

    2013-01-01

    Aloe barbadensis Miller (Aloe vera) is an herbal remedy promoted to treat a variety of illnesses; however, only limited data are available on the safety of this dietary supplement. Drinking water exposure of F344/N rats and B6C3F1 mice to an Aloe vera whole-leaf extract (1, 2, and 3%) for 13 weeks resulted in goblet cell hyperplasia of the large intestine in both species. Based upon this observation, 2-year drinking water studies were conducted to assess the carcinogenic potential of an Aloe vera whole-leaf extract when administered to F344/N rats (48 per sex per group) at 0.5, 1, and 1.5%, and B6C3F1 mice (48 per sex per group) at 1, 2, and 3%. Compared with controls, survival was decreased in the 1.5% dose group of female rats. Treatment-related neoplasms and nonneoplastic lesions in both species were confined primarily to the large intestine. Incidences of adenomas and/or carcinomas of the ileo-cecal and cecal-colic junction, cecum, and ascending and transverse colon were significantly higher than controls in male and female rats in the 1 and 1.5% dose groups. There were no neoplasms of the large intestine in mice or in the 0 or 0.5% dose groups of rats. Increased incidences of mucosa hyperplasia of the large intestine were observed in F344/N rats, and increased incidences of goblet cell hyperplasia of the large intestine occurred in B6C3F1 mice. These results indicate that Aloe vera whole-leaf extract is an intestinal irritant in F344/N rats and B6C3F1 mice and a carcinogen of the large intestine in F344/N rats.

  3. Estudo da viabilidade econômica do cultivo da babosa (aloe vera L. Study of economic viability of the Aloe vera L. culture

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    Dionizio Bernardino Bach

    2007-08-01

    Full Text Available Conduziu-se este este trabalho, com o objetivo de estudar a viabilidade econômica da babosa e verificar se o cultivo desta planta é ou não, uma alternativa economicamente viável ao produtor rural. Os dados relativos ao custo de produção, utilizados neste trabalho, foram obtidos junto às áreas de produção e instalações industriais da NATURAMA - Indústria e Comércio de Produtos Agropecuários Ltda, estabelecida no município de Paulo Lopes, SC. Na determinação do custo de produção de um hectare de babosa, em cultivo orgânico, foram consideradas todas as etapas, desde a obtenção das mudas até a conclusão do processamento, quando o produto final encontra-se apto ao consumo, e neste caso, é uma bebida em forma de suco. O processamento dos dados foi realizado utilizando uma planilha eletrônica desenvolvida especificamente para esse fim, a qual estimou os custos utilizando as metodologias do custo total (custos fixos e variáveis e do custo operacional total (custo operacional efetivo e depreciações. Pela análise da rentabilidade, conclui-se que a atividade é economicamente viável.The aim of this work is to study the economic viability of Aloe vera L. and verify if the culture of this plant is or not an alternative economically viable for the farmer. The data of production cost used in this work were gotten in the areas of production and industrial installations of NATURAMA- Industry and Trade of Farming Products LTDA, settled in Paulo Lopes, SC. In the determination of the production cost of one Aloe vera L hectare, all the phases were taken into consideration, from the getting of molts up to the conclusion of the processing, when the final product is ready to the consumption, and in this case, it's a beverage in a way of juice. The processing data was made by using an electronic spread sheet developed specifically for this reason, which estimated the costs using the methodologies of the total cost (steady and variable

  4. Aloe Vera External Use and Clinical Use Research Progress%芦荟临床外用研究进展

    Institute of Scientific and Technical Information of China (English)

    刘学华; 赵红影

    2011-01-01

    To research Aloe vera external use function of the clinical study on the review.Aloe vera has medical and health care, hairdressing, eating, and other functions, known as the "drug" and "natural beautician" and "family doctor", "miracle" laudatory name.In recent years, with the number of natural medicine research, Aloe become increasingly common attention of world medicine, especially the multipurpose development and research articles, application scope, increasing economic value.%对芦荟外用功能的临床应用研究进展进行了综述.芦荟具有医疗、保健、美容、食用、现赏等多种功能,近年来芦荟外用品的开发研究,倍受青睐,应用范围日趋扩大,成为全世界关注的药食多用植物.

  5. Growth Period Effects on the Protective Properties of Aloe vera Against t-BHP-Induced Oxidative Stress in Chang Cells.

    Science.gov (United States)

    Hwang, Jin-Woo; Kim, Eun-Kyung; Kim, Yon-Suk; Lee, Jae Woong; Lee, Jeong-Jun; Pyo, Han-Jong; Moon, Sang-Ho; Jeon, Byong-Tae; Park, Pyo-Jam

    2015-12-28

    Aloe vera has been used in traditional medicine for the therapy of a variety of disorders, such as wounds and burns. However, few studies have examined the antioxidant capacities of A. vera plants during different growth periods. In order to investigate the effects of growth on antioxidant activity, A. vera was prepared from 2-, 4-, 6-, 8-, and 12-month-old aloe. The extracts from 6-month-old A. vera showed the highest contents of flavonoids (9.750 mg catechin equivalent/g extract) and polyphenols (23.375 mg gallic acid equivalent/g extract) and the highest ferric reducing antioxidant power (0.047 mM ferrous sulfate equivalent/mg extract). The extract from 6-month-old A. vera exhibited the highest free radical scavenging potential, and the lowest IC50 values were found for 2,2-diphenyl-1-picrylhydrazyl (0.26 mg/ml) and alkyl radicals (0.50 mg/ml). In addition, the extract from 6-month-old A. vera showed the greatest effects on cell viability in normal liver cells. Based on these findings, the extract from 6-month-old A. vera was examined further in order to determine its protective potential against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress. The extract from 6-monthold A. vera at a concentration of 0.25 mg/ml showed the highest protective activity against t-BHP-induced reactive oxygen species production. These findings suggested that harvesting regimens were critical in the regulation of effects of the bioactive potential of A. vera on antioxidant activity.

  6. Antioxidant responses and salt stress tolerance of Aloe vera irrigated by seawater with different salinity

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The variations of antioxidant enzyme activities including superoxide dismutase (SOD: EC 1.15.1.1), peroxidase (POD: EC 1.11.1.7) and catalase (CAT: EC 1.11.1.6), lipid peroxidation and major electrolytes in Aloe vera irrigated for three years with seawater having different salinity were studied. The results indicate that POD activity increased significantly at 10% seawater level, whereas decreased at higher seawater levels. The SOD activity decreased with increasing seawater concentration except for treatment with 100% seawater (denoted as T100% ) under long-term salt stress. Salinity decreased CATactivity,and increased lipid peroxidation and cell membrane injury. In addition, Ca2+ content was high in Aloe irrigated by seawater of low salinity level, but low in Aloe irrigated by seawater of high salinity level. An opposite trend was observed for the effect of seawater on Na + content of plants. K + and Mg2 +contents remain relatively stable under various seawater levels, which benefit plant growth.

  7. Effects of fresh Aloe vera gel coating on browning alleviation of fresh cut wax apple (Syzygium samarangenese) fruit cv. Taaptimjaan.

    Science.gov (United States)

    Supapvanich, S; Mitrsang, P; Srinorkham, P; Boonyaritthongchai, P; Wongs-Aree, C

    2016-06-01

    The effect of natural coating by using fresh Aloe vera (A. vera) gel alleviating browning of fresh-cut wax apple fruits cv. Taaptimjaan was investigated. The fresh-cut fruits were dipped in fresh A. vera gel at various concentrations of 0, 25, 75 or 100 % (v/v) for 2 min at 4 ± 1 °C for 6 days. Lightness (L*), whiteness index (WI), browning index (BI), total color difference (ΔE*), sensorial quality attributes, total phenolic (TP) content, antioxidant activity and polyphenol oxidase (PPO) and peroxidase (POD) activities were determined. During storage, L* and WI of the fresh-cut fruits surface decreased whilst their BI and ΔE* increased. A. vera coating maintained the L* and WI and delayed the increase in BI and ΔE*, especially at 75 % A. vera dip. The fresh-cut fruits dipped in 75 % A. vera had the lowest browning score, the highest acceptance score and delayed the increase in TP content and PPO activity. However POD activity was induced by A. vera coating. Antioxidant activity had no effect on browning incidence of the fresh-cut fruits. Consequently, A. vera gel coating could maintain quality and retarded browning of fresh-cut wax apple fruits during storage.

  8. Automated Control of the Organic and Inorganic Composition of Aloe vera Extracts Using (1)H NMR Spectroscopy.

    Science.gov (United States)

    Monakhova, Yulia B; Randel, Gabriele; Diehl, Bernd W K

    2016-09-01

    Recent classification of Aloe vera whole-leaf extract by the International Agency for Research and Cancer as a possible carcinogen to humans as well as the continuous adulteration of A. vera's authentic material have generated renewed interest in controlling A. vera. The existing NMR spectroscopic method for the analysis of A. vera, which is based on a routine developed at Spectral Service, was extended. Apart from aloverose, glucose, malic acid, lactic acid, citric acid, whole-leaf material (WLM), acetic acid, fumaric acid, sodium benzoate, and potassium sorbate, the quantification of Mg(2+), Ca(2+), and fructose is possible with the addition of a Cs-EDTA solution to sample. The proposed methodology was automated, which includes phasing, baseline-correction, deconvolution (based on the Lorentzian function), integration, quantification, and reporting. The NMR method was applied to 41 A. vera preparations in the form of liquid A. vera juice and solid A. vera powder. The advantages of the new NMR methodology over the previous method were discussed. Correlation between the new and standard NMR methodologies was significant for aloverose, glucose, malic acid, lactic acid, citric acid, and WLM (P vera.

  9. Aloe vera Gel: Effective Therapeutic Agent against Multidrug-Resistant Pseudomonas aeruginosa Isolates Recovered from Burn Wound Infections

    Directory of Open Access Journals (Sweden)

    Mehdi Goudarzi

    2015-01-01

    Full Text Available Objective. Aloe vera is an herbal medicinal plant with biological activities, such as antimicrobial, anticancer, anti-inflammatory, and antidiabetic ones, and immunomodulatory properties. The purpose of this study was investigation of in vitro antimicrobial activity of A. vera gel against multidrug-resistant (MDR Pseudomonas aeruginosa isolated from patients with burn wound infections. Methods. During a 6-month study, 140 clinical isolates of P. aeruginosa were collected from patients admitted to the burn wards of a hospital in Tehran, Iran. Antimicrobial susceptibility test was carried out against the pathogens using the A. vera gel and antibiotics (imipenem, gentamicin, and ciprofloxacin. Results. The antibiogram revealed that 47 (33.6% of all isolates were MDR P. aeruginosa. The extract isolated from A. vera has antibacterial activity against all of isolates. Also, 42 (89.4% isolates were inhibited by A. vera gel extract at minimum inhibitory concentration (MIC ≤ 200 µg/mL. MIC value of A. vera gel for other isolates (10.6% was 800 µg/mL. All of MDR P. aeruginosa strains were inhibited by A. vera at similar MIC50 and MIC90 200 µg/mL. Conclusion. Based on our results, A. vera gel at various concentrations can be used as an effective antibacterial agent in order to prevent wound infection caused by P. aeruginosa.

  10. Aloe vera Gel: Effective Therapeutic Agent against Multidrug-Resistant Pseudomonas aeruginosa Isolates Recovered from Burn Wound Infections.

    Science.gov (United States)

    Goudarzi, Mehdi; Fazeli, Maryam; Azad, Mehdi; Seyedjavadi, Sima Sadat; Mousavi, Reza

    2015-01-01

    Objective. Aloe vera is an herbal medicinal plant with biological activities, such as antimicrobial, anticancer, anti-inflammatory, and antidiabetic ones, and immunomodulatory properties. The purpose of this study was investigation of in vitro antimicrobial activity of A. vera gel against multidrug-resistant (MDR) Pseudomonas aeruginosa isolated from patients with burn wound infections. Methods. During a 6-month study, 140 clinical isolates of P. aeruginosa were collected from patients admitted to the burn wards of a hospital in Tehran, Iran. Antimicrobial susceptibility test was carried out against the pathogens using the A. vera gel and antibiotics (imipenem, gentamicin, and ciprofloxacin). Results. The antibiogram revealed that 47 (33.6%) of all isolates were MDR P. aeruginosa. The extract isolated from A. vera has antibacterial activity against all of isolates. Also, 42 (89.4%) isolates were inhibited by A. vera gel extract at minimum inhibitory concentration (MIC) ≤ 200 µg/mL. MIC value of A. vera gel for other isolates (10.6%) was 800 µg/mL. All of MDR P. aeruginosa strains were inhibited by A. vera at similar MIC50 and MIC90 200 µg/mL. Conclusion. Based on our results, A. vera gel at various concentrations can be used as an effective antibacterial agent in order to prevent wound infection caused by P. aeruginosa.

  11. Effect of packaging methods and crude aloe vera gel (Aloe vera barbadensis miller) on the storage quality of enrobed goat meat bites.

    Science.gov (United States)

    Jairath, Gauri; Chatli, Manish K; Sahoo, Jhari; Biswas, Ashim K

    2015-06-01

    The storage stability of enrobed goat meat bites (EGMB) incorporated with 3 % crude aloe vera (AV) gel was evaluated under aerobic (T-1; unenrobed control product, T-2; enrobed AV treated product) and modified atmospheric packaging (MAP, 50:50, CO2 and N2) (T-3; unenrobed control product, T-4; enrobed AV treated product) at 4 ± 1 °C for 42 days on the basis of physico-chemical, microbiological and sensory attributes. The pH value was higher, whereas water activity (aW) was lower in enrobed and MAP product. Thiobarbituric acid reacting substances (TBARS) and free fatty acid (FFA) values were significantly (P Standard Plate Count (SPC) was significantly (P < 0.05) lower in MAP products than aerobic packaged products. Results concluded that EGMB treated with AV gel can be successfully stored more than 42 days under MAP conditions without affecting its physico-chemical, textural, microbiological and sensory attributes.

  12. 芦荟库拉索(Aloe vera L)再生体系的建立%Establishment of Plant Regeneration System for Aloe vera L.

    Institute of Scientific and Technical Information of China (English)

    吴英; 郭安平; 孔华; 贺立卡; 郭运玲

    2006-01-01

    对芦荟(Aloe vera L.)组织培养和再生体系进行了研究,初步获得芦荟的初代和继代培养基为MS+6-BA2.5 mg/L+NAA 0.2 mg/L+蔗糖30 mg/L,生根培养基成分为1/2MS+IBA 0.2 mg/L+NAA 0.1 mg/L+蔗糖30 mg/L,愈伤组织培养采用粗壮的芦荟基部的白色部分,诱导愈伤组织的培养基为B5+6-BA 2.0 mg/L+2,4-D 2.0 mg/L+蔗糖30 mg/L,采用Vc 5.0 mg/L抑制愈伤组织诱导过程中褐变的产生.诱导愈伤组织和外植体直接出芽2种方式并存.试管苗移栽后存活率为100%.采用潮霉素为抗性筛选标记,选择浓度为20~30 mg/L.

  13. Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers

    OpenAIRE

    A.P Sinurat; T Purwadaria; M.H Togatorop; T Pasaribu

    2003-01-01

    Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera ge...

  14. [Preparative isolation and purification of cinnamoyl-C-glycoside chromone from aloe vera by high-speed countercurrent chromatography].

    Science.gov (United States)

    Pan, Xia; Cao, Xueli; Dong, Yinmao; Zhao, Hua

    2005-01-01

    Aloe chromone is a group of anti-inflammatory and anti-tyrosinase constituents found in aloe vera leaves. High-speed countercurrent chromatography (HSCCC) is reported for the preparative isolation and purification of a chromone from aloe vera. The crude extract was obtained by a series of pretreatment of aloe vera leaves and extracted from decolorizing active carbon with methanol. Then the extract was distributed between dichloromethane and water, and the organic part was then subjected to HSCCC for the isolation of chromone constituents. The chromone compounds with a high performance liquid chromatographic grade (>95%) was isolated through two step HSCCC separations by employing two solvent systems composed of chloroform-methanol-water and dichloromethane-methanol-water at volume ratios of 4/3/2 and 5/4/2, respectively. The chromone was finally identified as cinnamoyl-C-glycoside chromone by ultraviolet (UV), fast atom bombardment mass spectrometry (FAB-MS), nuclear magnetic resonance (1H NMR and 13C NMR).

  15. Chemical and physical properties of aloe vera (Aloe barbadensis Miller gel stored after high hydrostatic pressure processing

    Directory of Open Access Journals (Sweden)

    Karina Di Scala

    2013-03-01

    Full Text Available The aim of this study was to evaluate the influence of high hydrostatic pressure (150, 250, 350, 450, and 550 MPa, applied for 5 minutes, on antioxidant capacity, total phenolic content, color, firmness, rehydration ratio, and water holding capacity of aloe vera gel stored for 60 days at 4 °C. The analyzed properties of the pressurized gel showed significant changes after the storage period. The highest value of total phenolic content was found at 550 MPa. However, a decrease in the antioxidant capacity was observed for all pressurized gel samples when compared to the control sample (p < 0.05. The smallest changes in product color were observed at pressure levels between 150 and 250 MP. The application of high hydrostatic pressure resulted in lower gel firmness, and the lowest value was found at 150 MPa (p < 0.05. On the other hand, the untreated sample showed a greater decrease in firmness, indicating that high pressure processing preserves this property. The application of high hydrostatic pressure exhibited modifications in the food matrix, which were evaluated in terms of rehydration ratio and water holding capacity.

  16. Nitric oxide production by chicken macrophages activated by Acemannan, a complex carbohydrate extracted from Aloe vera.

    Science.gov (United States)

    Karaca, K; Sharma, J M; Nordgren, R

    1995-03-01

    Cultures of normal chicken spleen cells and HD11 line cells produce nitric oxide (NO) in response to Acemannan, a complex carbohydrate derived from the Aloe vera plant. Neither cell type produced detectable amounts of NO in response to similar concentrations of yeast mannan, another complex carbohydrate. Nitric oxide production was dose dependent and inhibitable by the nitric oxide synthase inhibitor NG-methyl-L-arginine. In addition, the production of NO was inhibited by preincubation of ACM with concanavalin A in a dose-dependent manner. These results suggest that ACM-induced NO synthesis may be mediated through macrophage mannose receptors, and macrophage activation may be accountable for some of the immunomodulatory effects of ACM in chickens.

  17. Encapsulation of Aloe Vera extract into natural Tragacanth Gum as a novel green wound healing product.

    Science.gov (United States)

    Ghayempour, Soraya; Montazer, Majid; Mahmoudi Rad, Mahnaz

    2016-12-01

    Application of natural materials in wound healing is an interest topic due to effective treatment with no side effects. In this paper, Aloe Vera extract was encapsulated into Tragacanth Gum through a sonochemical microemulsion process to prepare a wound healing product. FESEM/EDX and FT-IR proved the successfully formation of the nanocapsules with spherical shape by cross-linking aluminum ions with Tragacanth Gum. The therapeutic characteristics of the prepared wound healing product were investigated using antimicrobial, cytotoxicity and wound healing assays. Relative high antimicrobial activities with the microbial reduction of 84, 91 and 80% against E. coli, S. aureus and C. albicans, a cell viability of 98% against human fibroblast cells and a good wound healing activity with considerable migration rate of fibroblast cells are the important advantages of the new formed wound healing product.

  18. Effect of vehicles on topical application of aloe vera and arnica montana components.

    Science.gov (United States)

    Bergamante, Valentina; Ceschel, Gian Carlo; Marazzita, Sergio; Ronchi, Celestino; Fini, Adamo

    2007-10-01

    In this study two types of gels and microemulsions are investigated for their ability to dissolve, release, and induce the permeation of helenalin, a flavonoid responsible for the anti-inflammatory activity of arnica montana extract, and aloin, an anthrone-C-glucosyls with antibacterial activity present in aloe vera extract. The release of these agents from each vehicle was followed by HPLC, and transcutaneous permeation was examined using a modified Franz cell and a porcine skin membrane. The study showed that a microemulsion can be a good vehicle to increase the permeation of helenalin, while the gel formulation, containing Sepigel 305, proved able to reduce the release and permeation of aloin, with a consequent activity limited to the surface of application, without any permeation. This is in accordance with the necessity to avoid this process, since human skin fibroblasts can metabolize absorbed aloin into a structurally related compound that increases the sensitivity of skin to ultraviolet light.

  19. Aloe vera bathing improved physical and humoral protection in breeding stock after induced spawning in matrinxã (Brycon amazonicus).

    Science.gov (United States)

    Zanuzzo, Fábio S; Zaiden, Sérgio F; Senhorini, José A; Marzocchi-Machado, Cleni M; Urbinati, Elisabeth C

    2015-07-01

    In this study, we show that induced spawning causes stress, an intense loss of epithelia and immunosuppression, decreasing physical and humoral protection in fish, effects that were prevented or improved in fish bathed with Aloe vera. A. vera has several medicinal properties, including wound healing and immunostimulatory effects, which we observed in this study. Fish bathed with A. vera had a higher number of epidermal goblet cells and, in general, an improved wound healing rate compared with the control after induced spawning. These effects might be related to (1) the stimulation of leukocyte activity, represented here by the increased leukocyte respiratory activity triggered by A. vera (leukocytes are recognized as playing an important role in wound repair); (2) the antimicrobial properties of A. vera, which decrease wound infection and accelerate the healing process; and (3) several mechanisms that explain the healing effect of A. vera (increased collagen synthesis, rate of epithelialization, and anti-inflammatory and moisturizing effects). Our results also suggest that caution is necessary during the induced spawning process, especially during stripping, and A. vera bathing is recommended after intensive aquaculture operations.

  20. Evaluation of Isolated Fractions of Aloe vera Gel Materials on Indinavir Pharmacokinetics: In vitro and in vivo Studies.

    Science.gov (United States)

    Wallis, Lonette; Malan, Maides; Gouws, Chrisna; Steyn, Dewald; Ellis, Suria; Abay, Efrem; Wiesner, Lubbe; Otto, Daniel P; Hamman, Josias

    2016-01-01

    Aloe vera is a plant with a long history of traditional medicinal use and is consumed in different products, sometimes in conjunction with prescribed medicines. A. vera gel has shown the ability to modulate drug absorption in vitro. The aim of this study was to fractionate the precipitated polysaccharide component of A. vera gel based on molecular weight and to compare their interactions with indinavir pharmacokinetics. Crude polysaccharides were precipitated from a solution of A. vera gel and was fractionated by means of centrifugal filtration through membranes with different molecular weight cut-off values (i.e. 300 KDa, 100 KDa and 30 KDa). Marker molecules were quantified in the aloe leaf materials by means of nuclear magnetic resonance spectroscopy and the average molecular weight was determined by means of gel filtration chromatography linked to multi-angle-laser-light scattering and refractive index detection. The effect of the aloe leaf materials on the transepithelial electrical resistance (TEER) of Caco-2 cell monolayers as well as indinavir metabolism in LS180 cells was measured. The bioavailability of indinavir in the presence and absence of the aloe leaf materials was determined in Sprague-Dawley rats. All the aloe leaf materials investigated in this study reduced the TEER of Caco-2 cell monolayers, inhibited indinavir metabolism in LS 180 cells to different extents and changed the bioavailability parameters of indinavir in rats compared to that of indinavir alone. These indinavir pharmacokinetic modulation effects were not dependent on the presence of aloverose and also not on the average molecular weight of the isolated fractions.

  1. Prevention of tri-nitrobenzene of sulfonic acid-induced colitis in chicken by using extract of Aloe vera

    Directory of Open Access Journals (Sweden)

    Motamed Elsayed Mahmoud

    Full Text Available Aim: Aloe vera, species of succulent plant in the genus Aloe, has multiple clinical activities and used routinely to accelerate wound healing. The present study was designed to investigate the anti-inflammatory effect of Aloe vera extracts (AVE in vitro and in vivo. Materials and Methods: The effect of crude AVE on inducible nitric oxide production by LPS/IFNg-stimulated cultured macrophages was evaluated. The therapeutic effect of administering crude Aloe vera extracts (100 mg/kg b.w. on the development of tri-nitrobenzene of sulfonic acid (TNBS-induced colitis (40 mg/kg b. w. in chicken was also investigated. Chicken is a valuable model for this purpose because it showed preference to bitter taste of Aloe vera. Diverse clinical pictures of the colitis including weight loss, diarrhea and histopathological changes were evaluated. Results: Nitrite production by LPS/IFNg-stimulated macrophages was maximally reduced by adding of AVE (100 μg/ml. This result suggests a direct inhibitory effect of AVE on the inflammatory cells. Chicks treated orally with AVE showed improvement of the histological signs with no inflammatory cell infiltrates and reduction of myeloperoxidase (MPO activities when compared with colitis control group. AVE pretreatment ameliorated significantly the clinical and histopathological severity of the TNBS-induced colitis; decreased body weight loss and diarrhea and increased survival. Conclusion: It was concluded that oral administration of AVE represents a valuable therapeutic approach for the treatment of colitis in chicken. [Vet. World 2012; 5(8.000: 469-476

  2. Healing effect of aloe vera gel,in non-healed ulcers

    Institute of Scientific and Technical Information of China (English)

    Majid Avijgan; Victoria Beigi Broujeni; Ali Akbar Beigi; Hojatollah Rohi Borojeni; Seyyed Ka-myar Mostafavizadeh; Iraj Karimi; Abbas Ali Javadi

    2009-01-01

    Objective:Aloe Vera gel (AVG)has a prominent role as a contemporary folk remedy.The Non-healed chron-ic ulcers of 16 non-responders to conventional treatment were treated with AVG for which the clinical efficacy was studied.Methods:This study included 16 patients having chronic ulcer (9 males and 7 females)with mean age of 41.8 ±14.6 who following failure treatment to conventional therapy,were dispensed AVG to ap-ply.AVG is consisted from gel part of Aloe Vera plant plus one preservative material,in this study ECG jelly. The patients were assessed as responder whenever intensity of inflammation,the number and /or the size of ul-cers have been reduced.Results:The period of time till healing (complete or reduction in size of ulcer), were from 5 to 160 days with mean of 31.2 ±36.4 days.The mean time of decreasing for secreting,swelling and redness was from 5-11 days.Spearman coefficient of correlation,showed no significant correlation between first size and depth of ulcer and timing till 50% reduction in size of ulcer.Conclusion:All patients had been treated with conventional treatment all to no avail.There were some full thickness ulcer which at the end of treatment we observed an improvement and normal skin for those ulcers.There was a minor complication like itching in one patient but no leading to stop treatment.These findings suggest that AVG is an excellent and sa-fer choice than current conventional therapeutic management of chronic ulcers.

  3. Effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing in rats

    Institute of Scientific and Technical Information of China (English)

    Kallaya Eamlamnam; Suthiluk Patumraj; Naruemon Visedopas; Duangporn Thong-Ngam

    2006-01-01

    AIM: To compare the effects of Aloe vera and sucralfate on gastric microcirculatory changes, cytokine levels and gastric ulcer healing.METHODS: Male Spraque-Dawley rats (n=48) were divided into four groups. Group1 served as control group,group 2 as gastric ulcer group without treatment, groups 3 and 4 as gastric ulcer treatment groups with sucralfate and Aloe vera. The rats from each group were divided into 2 subgroups for study of leukocyte adherence, TNF-α and IL-10 levels and gastric ulcer healing on days 1 and 8 after induction of gastric ulcer by 20% acetic acid. RESULTS: On day 1 after induction of gastric ulcer, the leukocyte adherence in postcapillary venule was significantly (P< 0.05) increased in the ulcer groups when compared to the control group. The level of TNF-αwas elevated and the level of IL-10 was reduced. In the ulcer groups treated with sucralfate and Aloe vera,leukocyte adherence was reduced in postcapillary venule.The level of IL-10 was elevated, but the level of TNF-αhad no significant difference. On day 8, the leukocyte adherence in postcapillary venule and the level of TNF-αwere still increased and the level of IL-10 was reduced in the ulcer group without treatment. The ulcer treated with sucralfate and Aloe vera had lower leukocyte adherence in postcapillary venule and TNF-α level. The level of IL-10 was still elevated compared to the ulcer group without treatment. Furthermore, histopathological examination of stomach on days 1 and 8 after induction of gastric ulcer showed that gastric tissue was damaged with inflammation. In the ulcer groups treated with sucralfate and Aloe vera on days 1 and 8, gastric inflammation was reduced, epithelial cell proliferation was enhanced and gastric glands became elongated. The ulcer sizes were also reduced compared to the ulcer group without treatment.CONCLUSION: Administration of 20% acetic acid can induce gastric inflammation, increase leukocyte adherence in postcapillary venule and TNF-α level

  4. In vivo safety evaluation of UP780, a standardized composition of aloe chromone aloesin formulated with an Aloe vera inner leaf fillet.

    Science.gov (United States)

    Yimam, Mesfin; Brownell, Lidia; Jia, Qi

    2014-08-01

    Safety profiles of the aloe chromone aloesin or Aloe vera inner leaf fillet (Qmatrix) as a well tolerated entity have been reported separately. UP780, a standardized composition of aloe chromone formulated with an Aloe vera inner leaf fillet, has shown a significant beneficial effect in lowering blood glucose and improving insulin resistance in human. Here we evaluate the safety of UP780 after a repeated 14 and 90-day oral administration in CD-1 mice. UP780 was given at doses of 100mg/kg/day, 500mg/kg/day and 1000mg/kg/day to groups of 10 male and 10 female for 90days or administered by oral gavage at a dose of 2g/kg/day to groups of 5 male and 5 female for 14days. Body weight, feed consumption, hematology, clinical chemistry and histopathologic evaluation were performed. UP780 at a dose of 1000mg/kg/day or at 2000mg/kg/day produced no treatment-related toxicity or mortality. Body weight gain or feed consumption was similar between groups. There was no test article-related microscopic change. Spontaneously occurring minor changes in clinical chemistry and hematology were observed. However, these changes were limited to one sex or were not dose correlated. UP780 was well tolerated in this strain. A dose of 2000mg/kg/day was identified as the NOAEL (no-observed-adverse-effect-level).

  5. Metagenomics study of endophytic bacteria in Aloe vera using next-generation technology

    Directory of Open Access Journals (Sweden)

    Mushafau Adewale Akinsanya

    2015-12-01

    Full Text Available Next generation sequencing (NGS enables rapid analysis of the composition and diversity of microbial communities in several habitats. We applied the high throughput techniques of NGS to the metagenomics study of endophytic bacteria in Aloe vera plant, by assessing its PCR amplicon of 16S rDNA sequences (V3–V4 regions with the Illumina metagenomics technique used to generate a total of 5,199,102 reads from the samples. The analyses revealed Proteobacteria, Firmicutes, Actinobacteria and Bacteriodetes as the predominant genera. The roots have the largest composition with 23% not present in other tissues. The stems have more of the genus—Pseudomonas and the unclassified Pseudomonadaceae. The α-diversity analysis indicated the richness and inverse Simpson diversity index of the bacterial endophyte communities for the leaf, root and stem tissues to be 2.221, 6.603 and 1.491 respectively. In a similar study on culturable endophytic bacteria in the same A. vera plants (unpublished work, the dominance of Pseudomonas and Bacillus genera was similar, with equal proportion of four species each in root, stem and leaf tissues. It is evident that NGS technology captured effectively the metagenomics of microbiota in plant tissues and this can improve our understanding of the microbial–plant host interactions.

  6. Diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in Aloe vera.

    Science.gov (United States)

    Akinsanya, Mushafau Adewale; Goh, Joo Kheng; Lim, Siew Ping; Ting, Adeline Su Yien

    2015-12-01

    Twenty-nine culturable bacterial endophytes were isolated from surface-sterilized tissues (root, stem and leaf) of Aloe vera and molecularly characterized to 13 genera: Pseudomonas, Bacillus, Enterobacter, Pantoea, Chryseobacterium, Sphingobacterium, Aeromonas, Providencia, Cedecea, Klebsiella, Cronobacter, Macrococcus and Shigella. The dominant genera include Bacillus (20.7%), Pseudomonas (20.7%) and Enterobacter (13.8%). The crude and ethyl acetate fractions of the metabolites of six isolates, species of Pseudomonas, Bacillus, Chryseobacterium and Shigella, have broad spectral antimicrobial activities against pathogenic Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Salmonella Typhimurium, Proteus vulgaris, Klebsiella pneumoniae, Escherichia coli, Streptococcus pyogenes and Candida albicans, with inhibition zones ranging from 6.0 ± 0.57 to 16.6 ± 0.57 mm. In addition, 80% of the bacterial endophytes produced 1,1-diphenyl-2-picrylhydrazyl (DPPH) with scavenging properties of over 75% when their crude metabolites were compared with ascorbic acid (92%). In conclusion, this study revealed for the first time the endophytic bacteria communities from A. vera (Pseudomonas hibiscicola, Macrococcus caseolyticus, Enterobacter ludwigii, Bacillus anthracis) that produce bioactive compounds with high DPPH scavenging properties (75-88%) and (Bacillus tequilensis, Pseudomonas entomophila, Chryseobacterium indologenes, Bacillus aerophilus) that produce bioactive compounds with antimicrobial activities against bacterial pathogens. Hence, we suggest further investigation and characterization of their bioactive compounds.

  7. Aloe Vera

    Science.gov (United States)

    63053 ... https://nccih.nih.gov/health/aloevera ... us ... 63053 ... https://nccih.nih.gov/health/aloevera ... Burns ... Burns/NON-DISPLAY (Supplements page) ... us ... 63053 ... https://nccih.nih. ...

  8. Polymer fraction of Aloe vera exhibits a protective activity on ethanol-induced gastric lesions.

    Science.gov (United States)

    Park, Chul-Hong; Nam, Dong-Yoon; Son, Hyeong-U; Lee, Si-Rim; Lee, Hyun-Jin; Heo, Jin-Chul; Cha, Tae-Yang; Baek, Jin-Hong; Lee, Sang-Han

    2011-04-01

    For centuries, Aloe has been used as a herbal plant remedy against skin disorders, diabetes, and for its cardiac stimulatory activity. Here, we examined the gastroprotective effects of an Aloe vera polymer fraction (Avpf; molecular weight cut-off ≥50 kDa; 150 mg/kg body weight, p.o.) on an ethanol-induced gastric lesion mouse model. Mice pre-treated with Avpf had significantly fewer gastric lesions than their respective controls. To further examine the potential mechanism underlying this effect, we used reverse transcription-polymerase chain reaction to examine nitric oxide synthase and matrix metalloproteinase (MMP)mRNA expression on tissues from gastric lesions. Our results revealed that the mRNA expressions of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS) were each reduced by ~50% in Avpf-treated mice vs. the controls, whereas, the mRNA expression levels of endothelial nitric oxide synthase remained unchanged. MMP-9, an index for gastric lesions, also alleviated the ethanol-treated gastric ulceration during Avpf treatment. These findings collectively suggest that Avpf significantly protects the gastric mucosa against ethanol-induced gastric damage, at least in part, by decreasing mRNA expression levels of not only iNOS and nNOS, but also MMP-9.

  9. Toxicologic Assessment of a Commercial Decolorized Whole Leaf Aloe Vera Juice, Lily of the Desert Filtered Whole Leaf Juice with Aloesorb

    Directory of Open Access Journals (Sweden)

    Inder Sehgal

    2013-01-01

    Full Text Available Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies—examined grossly and microscopically—that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  10. Toxicologic assessment of a commercial decolorized whole leaf aloe vera juice, lily of the desert filtered whole leaf juice with aloesorb.

    Science.gov (United States)

    Sehgal, Inder; Winters, Wallace D; Scott, Michael; David, Andrew; Gillis, Glenn; Stoufflet, Thaya; Nair, Anand; Kousoulas, Konstantine

    2013-01-01

    Aloe vera, a common ingredient in cosmetics, is increasingly being consumed as a beverage supplement. Although consumer interest in aloe likely stems from its association with several health benefits, a concern has also been raised by a National Toxicology Program Report that a nondecolorized whole leaf aloe vera extract taken internally by rats was associated with intestinal mucosal hyperplasia and ultimately malignancy. We tested a decolorized whole leaf (DCWL) aloe vera, treated with activated charcoal to remove the latex portion of the plant, for genotoxicity in bacteria, acute/subacute toxicity in B6C3F1 mice, and subchronic toxicity in F344 rats. We found this DCWL aloe vera juice to be nongenotoxic in histidine reversion and DNA repair assays. Following acute administration, mice exhibited no adverse signs at 3- or 14-day evaluation periods. When fed to male and female F344 rats over 13 weeks, DCWL aloe led to no toxicity as assessed by behavior, stools, weight gain, feed consumption, organ weights, and hematologic or clinical chemistry profiles. These rats had intestinal mucosal morphologies-examined grossly and microscopically-that were similar to controls. Our studies show that oral administration of this DCWL aloe juice has a different toxicology profile than that of the untreated aloe juice at exposures up to 13 weeks.

  11. Mineral content and biochemical variables of Aloe vera L. under salt stress.

    Science.gov (United States)

    Murillo-Amador, Bernardo; Córdoba-Matson, Miguel Víctor; Villegas-Espinoza, Jorge Arnoldo; Hernández-Montiel, Luis Guillermo; Troyo-Diéguez, Enrique; García-Hernández, José Luis

    2014-01-01

    Despite the proven economic importance of Aloe vera, studies of saline stress and its effects on the biochemistry and mineral content in tissues of this plant are scarce. The objective of this study was to grow Aloe under NaCl stress of 0, 30, 60, 90 and 120 mM and compare: (1) proline, total protein, and enzyme phosphoenolpyruvate carboxylase (PEP-case) in chlorenchyma and parenchyma tissues, and (2) ion content (Na, K, Ca, Mg, Cl, Fe, P. N, Zn, B, Mn, and Cu) in roots, stems, leaves and sprouts. Proline and PEP-case increased as salinity increased in both parenchyma and chlorenchyma, while total protein increased in parenchyma and decreased in chlorenchyma, although at similar salt concentrations total protein was always higher in chlorenchyma. As salinity increased Na and Cl ions increased in roots, stems, leaves, while K decreased only significantly in sprouts. Salinity increases typically caused mineral content in tissue to decrease, or not change significantly. In roots, as salinity increased Mg decreased, while all other minerals failed to show a specific trend. In stems, the mineral concentrations that changed were Fe and P which increased with salinity while Cu decreased. In leaves, Mg, Mn, N, and B decreased with salinity, while Cu increased. In sprouts, the minerals that decreased with increasing salinity were Mg, Mn, and Cu. Zinc did not exhibit a trend in any of the tissues. The increase in protein, proline and PEP-case activity, as well as the absorption and accumulation of cations under moderate NaCl stress caused osmotic adjustment which kept the plant healthy. These results suggest that Aloe may be a viable crop for soil irrigated with hard water or affected by salinity at least at concentrations used in the present study.

  12. Effect of Aloe vera application on the content and molecular arrangement of glycosaminoglycans during calcaneal tendon healing.

    Science.gov (United States)

    Aro, Andrea Aparecida de; Esquisatto, Marcelo Augusto Marretto; Nishan, Umar; Perez, Mylena Oliveira; Rodrigues, Rodney Alexandre Ferreira; Foglio, Mary Ann; Carvalho, João Ernesto de; Gomes, Laurecir; Vidal, Benedicto De Campos; Pimentel, Edson Rosa

    2014-12-01

    Although several treatments for tendon lesions have been proposed, successful tendon repair remains a great challenge for orthopedics, especially considering the high incidence of re-rupture of injured tendons. Our aim was to evaluate the pharmacological potential of Aloe vera on the content and arrangement of glycosaminoglycans (GAGs) during tendon healing, which was based on the effectiveness of A. vera on collagen organization previously observed by our group. In rats, a partial calcaneal tendon transection was performed with subsequent topical A. vera application at the injury site. The tendons were treated with A. vera ointment for 7 days and excised on the 7(th) , 14(th) , or 21(st) day post-surgery. Control rats received ointment without A. vera. A higher content of GAGs and a lower amount of dermatan sulfate were detected in the A. vera-treated group on the 14(th) day compared with the control. Also at 14 days post-surgery, a lower dichroic ratio in toluidine blue stained sections was observed in A. vera-treated tendons compared with the control. No differences were observed in the chondroitin-6-sulfate and TGF-β1 levels between the groups, and higher amount of non-collagenous proteins was detected in the A. vera-treated group on the 21(st) day, compared with the control group. No differences were observed in the number of fibroblasts, inflammatory cells and blood vessels between the groups. The application of A. vera during tendon healing modified the arrangement of GAGs and increased the content of GAGs and non-collagenous proteins.

  13. Study of Sedative-Hypnotic Effects of Aloe vera L. Aqueous Extract through Behavioral Evaluations and EEG Recording in Rats.

    Science.gov (United States)

    Abdollahnejad, Fatemeh; Mosaddegh, Mahmoud; Nasoohi, Sanaz; Mirnajafi-Zadeh, Javad; Kamalinejad, Mohammad; Faizi, Mehrdad

    2016-01-01

    In this study, we investigated the sedative and hypnotic effects of the aqueous extract of Aloe vera on rats. In order to evaluate the overall hypnotic effects of the Aloe vera extract, open field and loss of righting reflex tests were primarily used. The sedative and hypnotic effects of the extract were then confirmed by detection of remarkable raise in the total sleeping time through analysis of electroencephalographic (EEG) recordings of animals. Analysis of the EEG recordings showed that there is concomitant change in Rapid Eye Movement (REM) and None Rapid Eye Movement (NREM) sleep in parallel with the prolonged total sleeping time. Results of the current research show that the extract has sedative-hypnotic effects on both functional and electrical activities of the brain.

  14. Effect of Heat Shock Treatment and Aloe Vera Coating to Chilling Injury Symptom in Tomato (Lycopersicon asculantum Mill.

    Directory of Open Access Journals (Sweden)

    Sutrisno

    2012-04-01

    Full Text Available This research was undertaken to determine the effect of length in heat shock and edible coating as pre-storage treatment to Chilling Injury (CI symptom reflected by ion leakage induced and quality properties in tomato (Lycopersicon asculantum Mill.. Heat Shock Treatment (HST was conducted at three different levels of length, which were, 20; 40 and 60 min. Edible coating was conducted using aloe vera gel. The result showed that HST and Aloe Vera Coating (AVC were more effective to reduce CI symptom at lower chilling storage. Prolong exposure to heated water may delay climacteric peak. The length of heat shock, AVC treatment and low temperature storage significantly affected the tomato quality parameter but not significantly different for each treatment except weight loss. HST for 20 min at ambient temperature was significantly different to other treatment.

  15. 芦荟对果蝇寿命的影响%Effects of Aloe vera L on life of Drosophila

    Institute of Scientific and Technical Information of China (English)

    马红; 王红星

    2008-01-01

    以药用价值较高的库拉索芦荟(Aloe vera L.Burm.f)为材料,研究了芦荟叶的不同部分对果蝇寿命及生活周期的影响.结果表明,芦荟全叶和芦荟凝胶能明显延长果蝇的寿命、缩短生长周期.

  16. Aloe vera Leaf Anti Inflamation’s Activity Speeds Up the Healing Proccess of Oral Mucosa Ulceration

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    Agoeng Tjahajani

    2013-05-01

    Full Text Available Previous work showed that 25% of inner layer Aloe vera leaf extract was effective as anti-inflammatory on the oral mucous of Wistar rats. Objective: The aim of this study was to examine the lowest concentration of whole leaf Aloe vera extract that could accelerate healing process of ulceration on rats. Methods: Sixty inbreeding Spraque Dawley rats was exposed to solution of Hydrogen Peroxide 10% during three days to induced inflammation on the labial mucous inferior of rats. During the next three days, on the same area of control group were topically applied solution of Natrium Chloride 0.9%; while in the treatment group were topically applied 6.25%; 12.5%; 25% of whole leaf of Aloe vera extract for 3x5 minutes with 90 minutes intervals. On the fourth day, five rats in each group were sacrificed; and the same treatment was done on others groups. On the sixth day, five rats of each group were sacrificed; and the same treatment was done on the rest group up to seven days. On the eighth days, the rest groups were sacrificed. Microscopic slides were done. Results: Microscopic slides were analyzed under light microscope and scored. Statistical analysis with Mann-Whitney test showed significant differences between control and treatment group (p<0.05. Conclusion: Application of 6.25% whole leaf Aloe vera extract was the lowest and the most effective concentration in accelerating the healing process of oral mucous ulceration on rats.DOI: 10.14693/jdi.v18i1.56

  17. Comparative study of the efficacy of lycopene and aloe vera in the treatment of oral submucous fibrosis

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    Santosh Patil

    2015-01-01

    Full Text Available Background and Aim: Oral submucous fibrosis (OSMF is a high-risk premalignant condition largely seen in the Indian subcontinent. A number of studies have proven the use of antioxidants in the management of OSMF. Therefore, the aim of the present study was to compare the efficacy of two antioxidants, lycopene and aloe vera in the management of OSMF. Material and Methods: One hundred and twenty clinicopathologically diagnosed OSMF patients, were included in the study. They were divided equally into, Group A (lycopene group and Group B (aloe vera group. Group A was administered 8mg lycopene in two divided doses of 4mg daily and Group B was given5mg aloe vera gel to be applied topically thrice daily for 3 months. Different clinical parameters were evaluated at regular intervals and data was analyzed using the Student′s paired t-test and Chi-square test. P < 0.001 was considered to be statistically significant. Results: Clinical improvements in mouth opening and tongue protrusion were significant in Group A (P < 0.001. Subjective symptoms of burning sensation (P = 0.007, pain associated with the lesion (P = 0.005, and difficulty in swallowing and speech (P = 0.003 improved in both the groups, but were insignificant. There was a mild to moderate decrease in the size of the lesion. Conclusion: The present study concludes that though, there is no definitive treatment for the condition; however, lycopene can bring about significant clinical improvements in the symptoms like mouth opening and tongue protrusion when compared to aloe vera. Both the drugs appear to be promising in the treatment of OSMF.

  18. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity

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    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Subash C B Gopinath; Chen, Yeng; Pandian, Kannaiyan

    2016-01-01

    Abstract Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffrac...

  19. Development of novel wound care systems based on nanosilver nanohydrogels of polymethacrylic acid with Aloe vera and curcumin

    Energy Technology Data Exchange (ETDEWEB)

    Anjum, Sadiya [Bioengineering Laboratory, Department of Textile Technology, Indian Institute of Technology, New Delhi 110016 (India); Gupta, Amlan; Sharma, Deepika [Department of Pathology, Sikkim Manipal Institute of Medical Sciences, Sikkim Manipal University, Gangtok 737102 (India); Gautam, Deepti [Bioengineering Laboratory, Department of Textile Technology, Indian Institute of Technology, New Delhi 110016 (India); Bhan, Surya; Sharma, Anupama [Department of Biochemistry, North Eastern Hill University, Shillong 793022, Meghalaya (India); Kapil, Arti [Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029 (India); Gupta, Bhuvanesh, E-mail: bgupta@textile.iitd.ernet.in [Bioengineering Laboratory, Department of Textile Technology, Indian Institute of Technology, New Delhi 110016 (India)

    2016-07-01

    This study is aimed at the development of a composite material for wound dressing containing nanosilver nanohydrogels (nSnH) along with Aloe vera and curcumin that promote antimicrobial nature, wound healing and infection control. Nanosliver nanohydrogels were synthesized by nanoemulsion polymerization of methacrylic acid (MAA) followed by subsequent crosslinking and silver reduction under irradiation. Both the polymerization and irradiation time had significant influence on the nanoparticle shape, size and its formation. Polyvinyl alcohol/polyethylene oxide/carboxymethyl cellulose matrix was used as gel system to blend with nSnH, A. vera, curcumin and coat it on the hydrolysed PET fabric to develop antimicrobial dressings. The cumulative release of silver from the dressing was found to be ~ 42% of the total loading after 48 h. The antimicrobial activity of the dressings was studied against both Staphylococcus aureus and Escherichia coli. In vivo wound healing studies were carried out over a period of 16 d on full-thickness skin wounds created on Swiss albino mice. Fast healing was observed in Gel/nSnH/Aloe treated wounds with minimum scarring, as compared to other groups. The histological studies showed A. vera based dressings to be the most optimum one. These results suggest that nSnH along with A. vera based dressing material could be promising candidates for wound dressings. - Highlights: • PMAA based nanosilver nanogels offer excellent antimicrobial property. • nSnH may be coated on a fabric surface to develop wound care dressings. • Dressings show good wound recovery on animal models. • Aloe vera shows better healing behaviour as compared to the curcumin based systems.

  20. Determinación del efecto del gel de Aloe vera, sobre la glicemia en caninos sanos

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    María Mercedes Tamayo

    2011-02-01

    Full Text Available El papel desempeñado por la glucosa en los organismos es  fundamental, ya que es  la forma más importante por la cual los carbohidratos son absorbidos desde el tracto intestinal, con diversos factores que  influyen en los niveles de glucosa del  organismo.  La  causa más  frecuente  de hiperglucemia  en  los  perros  es  el  daño pancreático, hiperadrenocorticismo  e hipersomatotropismo. Así, al evaluar el efecto del gel de Aloe vera sobre  la glicemia en caninos,tras su administración oral, se utilizaron 18 ejemplares sanos que  fueron distribuidos al azar en seis grupos, en los que se administró diferentes sustancias y se procedió a realizar el exámen de glicemia cada 15 minutos  (min, obteniéndose que la administración de 50 mL de gel de Aloe vera, tanto a caninos normoglicémicos, como a los que se les indujo hiperglucemia, no produjo cambios significativos en los niveles de glucosa.Los  resultados obtenidos  bajo  las  condiciones planteadas en este estudio experimental no validaron el uso empírico tradicional del gel de Aloe vera.

  1. Polysaccharides of Aloe vera induce MMP-3 and TIMP-2 gene expression during the skin wound repair of rat.

    Science.gov (United States)

    Tabandeh, Mohammad Reza; Oryan, Ahmad; Mohammadalipour, Adel

    2014-04-01

    Polysaccharides are the main macromolecules of Aloe vera gel but no data about their effect on extracellular matrix (ECM) elements are available. Here, mannose rich Aloe vera polysaccharides (AVP) with molecular weight between 50 and 250 kDa were isolated and characterized. Open cutaneous wounds on the back of 45 rats (control and treated) were daily treated with 25mg (n=15) and 50 mg (n=15) AVP for 30 days. The levels of MMP-3 and TIMP-2 gene expression were analyzed using real time PCR. The levels of n-acetyl glucosamine (NAGA), n-acetyl galactosamine (NAGLA) and collagen contents were also measured using standard biochemical methods. Faster wound closure was observed at day 15 post wounding in AVP treated animals in comparison with untreated group. At day 10 post wounding, AVP inhibited MMP-3 gene expression, while afterwards MMP-3 gene expression was upregulated. AVP enhanced TIMP-2 gene expression, collagen, NAGLA and NAGA synthesis in relation to untreated wounds. Our results suggest that AVP has positive effects on the regulation of ECM factor synthesis, which open up new perspectives for the wound repair activity of Aloe vera polysaccharide at molecular level.

  2. Haemato-immunological indices in rainbow trout (Oncorhynchus mykiss) fry fed with Aloe vera extract supplemented feed

    Institute of Scientific and Technical Information of China (English)

    Masoud Haghighi; Mostafa Sharif Rohani; Hamid Pourmoghim; Tayebeh Toliat; Meysam Samadi; Meysam Tavoli; Maryam Islami; Rahmat Yusefi

    2014-01-01

    Objective: To evaluate the effects of Aloe vera extract on the immunity responses and haematological parameters in rainbow trout (Oncorhynchus mykiss) fry to develop alternative drug to chemotherapeutics in aquaculture. Methods:Six hundred rainbow trout (Oncorhynchus mykiss) fry were randomly allocated into two treatment groups including 1) placebo-treated group (control), 2) Aloe vera extract-treated group, each of three replicates. The fishes were hand-fed once a day with diet medicated AE or placebo at the rate of 1%in feed in the first feeding for 10 weeks. At the end of the identical every two weeks 24 h after feeding, some of haematological and immunological parameters were analyzed. Results:The results showed that serum total protein, albumin and globulin, respiratory burst activity, phagocytic activity and serum lysozyme activity vary among the two treatment groups which were found to be higher in Aloe vera extract-treated group (P0.05). Conclusions: It was concluded that supplementation of AE at a rate of 1%in feed registered higher immunological responses in compared to placebo group. Therefore, supplementation of AE in fish diets enhances non-specific immune system in fish. It may use in fish diets particularly at time of outbreaks.

  3. Mechanical, Thermal, and Morphological Properties of Nanocomposites Based on Polyvinyl Alcohol and Cellulose Nanofiber from Aloe vera Rind

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    Adel Ramezani Kakroodi

    2014-01-01

    Full Text Available This work was devoted to reinforcement of polyvinyl alcohol (PVA using cellulose nanofibers from Aloe vera rind. Nanofibers were isolated from Aloe vera rind in the form of an aqueous suspension using chemimechanical technique. Mechanical characterizations showed that incorporation of even small amounts of nanofibers (as low as 2% by weight had significant effects on both the modulus and strength of PVA. Tensile modulus and strength of PVA increased, 32 and 63%, respectively, after adding 2% of cellulose nanofiber from Aloe vera rind. Samples with higher concentrations of nanofibers also showed improved mechanical properties due to a high level of interfacial adhesion and also dispersion of fibers. The results showed that inclusion of nanofibers decreased deformability of PVA significantly. Dynamic mechanical analysis revealed that, at elevated temperatures, improvement of mechanical properties due to the presence of nanofibers was even more noticeable. Addition of nanofibers resulted in increased thermal stability of PVA in thermogravimetric analysis due to the reduction in mobility of matrix molecules. Morphological observations showed no signs of agglomeration of fibers even in composites with high cellulose nanofiber contents. Inclusion of nanofibers was shown to increase the density of composites.

  4. Antimutagenicity and Anticarcinogenic Effects of Gel and Latex Extracts of Aloe Vera Cultivated: A Comparative Study in Two Cities, Iran

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    Kheiri A

    2012-03-01

    Full Text Available Background: Nowadays, cancer is one of the main causes of mortality in the world and many mutagens are the cause of death in millions of patients. Due to the side effects of anticancer drugs, scientists are in search of natural drugs with fewer side effects and more therapeutic efficacy. This study aims to, firstly, investigate the antimutgenic effects of different Aloe vera gel and latex extracts on mutated Salmonella typhimurium bacterium by using Ames test and to, secondly, study the probable effects of the habitat conditions on the antimutagenic effects of the plant.Methods: After preparing different Aloe vera gel and latex extracts, the antimutagenic effects of the extracts were evaluated by Ames test. In this test, a mutated strain of S. typhimurium was grown on culture media containing a minimum of salt and glucose in the presence of a mutagen substance (NaN3. Subsequently, only those bacteria that had turned HIS+ by reverse mutation formed colonies. As different alcoholic and aqueous extracts of Aloe vera reduced reversed mutations, the difference between the means of revertant mutants per plate was calculated by one-way ANOVA using SPSS software (version 18. Results: The ethanol extracts of latex from Karaj had a maximum (91% and aqueous extract from Dezfoul had a minimum (42% percentage of inhibition. Conclusion: Maximum percentage of inhibition was observed in the extracts of the plant cultivated in Karaj reflecting the impact of environmental conditions on the construction of antioxidant compounds in plants.

  5. The Aloe vera phenomenon: a review of the properties and modern uses of the leaf parenchyma gel.

    Science.gov (United States)

    Grindlay, D; Reynolds, T

    1986-06-01

    The mucilaginous gel from the parenchymatous cells in the leaf pulp of Aloe vera has been used since early times for a host of curative purposes. This gel should be distinguished clearly from the bitter yellow exudate originating from the bundle sheath cells, which is used for its purgative effects. Aloe vera gel has come to play a prominent role as a contemporary folk remedy, and numerous optimistic, and in some cases extravagant, claims have been made for its medicinal properties. Modern clinical use of the gel began in the 1930s, with reports of successful treatment of X-ray and radium burns, which led to further experimental studies using laboratory animals in the following decades. The reports of these experiments and the numerous favourable case histories did not give conclusive evidence, since although positive results were usually described, much of the work suffered from poor experimental design and insufficiently large test samples. In addition some conflicting or inconsistent results were obtained. With the recent resurgence of interest in Aloe vera gel, however, new experimental work has indicated the possibility of distinct physiological effects. Chemical analysis has shown the gel to contain various carbohydrate polymers, notably either glucomannans or pectic acid, along with a range of other organic and inorganic components. Although many physiological properties of the gel have been described, there is no certain correlation between these and the identified gel components.

  6. Antimicrobial capacity of Aloe vera and propolis dentifrice against Streptococcus mutans strains in toothbrushes: an in vitro study

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    Patrícia Fernanda Roesler Bertolini

    2012-02-01

    Full Text Available OBJECTIVES: This study evaluated in vitro the efficiency of Aloe vera and propolis dentifrice on reducing the contamination of toothbrush bristles by a standard strain of Streptococcus mutans (ATCC 25175; SM, after toothbrushing. MATERIAL AND METHODS: Fifteen sterile toothbrushes were randomly divided into 5 toothbrushing groups: I (negative control: without dentifrice; II: with fluoridated dentifrice; III: with triclosan and gantrez dentifrice; IV (positive control: without dentifrice and irrigation with 10 mL of 0.12% chlorhexidine gluconate; V: with Aloe vera and propolis dentifrice. In each group, 1 sterile bovine tooth was brushed for 1 min, where the toothbrush bristles were contaminated with 25 µL of SM. After toothbrushing, the bristles were stored in individual test tubes with 3 mL of BHI under anaerobiosis of 37°C for 48 h. Then, they were seeded with sterile swab in triplicate in the Mitis salivarius - Bacitracin culture medium. The samples were kept under anaerobiosis of 37°C for 48 h. Scores were used to count the number of colony forming units (cfu. The results were submitted to the Mann-Whitney statistical test at 5% significance level. RESULTS: There was statistically significant difference (p<0.05 for the reduction of bristle contamination comparing groups II, III, IV and V to group I. CONCLUSIONS: It may be stated that after toothbrushing, the Aloe vera and propolis dentifrice reduced the contamination of toothbrush bristles by SM, without differentiation from the other chemical agents used.

  7. Effect of Aloe Vera mouthwash on dental plaque index in patients with endotracheal tube hospitalized in intensive care unite

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    Nasrin Mirbastegan

    2016-06-01

    Full Text Available Introduction: Oral health is an important aspect of caring for hospitalized patients in intensive care unit (ICU. Typically, 48 hours after admission to Intensive care unit, there is a change in mouth flora and dental plaque will be created subsequently. These events make an ideal environment for microbial growth. The aim of this study was to determine the effect of Aloe Vera mouthwash on dental plaque index in patients with tracheal intubation at ICU. Material and Methods: This randomized double blind trail was performed on 79 patients with Tracheal intubation at ICU. Data were gathered using purposive sampling. Participant divided in to the two groups. The experimental group received Aloe Vera mouth wash and control group received Normal Saline mouth wash. The study period was four days. O’leary Plaque index used for collecting data. Kolmogorov-Smirnov, Chi square, paired t-test and independent ttest were used for analyzing data by SPSS 20. Result: The experimental and control groups had similar characteristics except gender. The experimental dental plaque index (56.58±11.91 and Control dental plaque index (56.04±10.46 were similar. The dental plaque index had a significant decrease in experimental group (57.51±9.08 comparing to the control group (62.46±9.46. Conclusion: Aloe Vera mouth wash along with tooth brushing have a significant effect on reducing dental plaque index.

  8. Development of novel wound care systems based on nanosilver nanohydrogels of polymethacrylic acid with Aloe vera and curcumin.

    Science.gov (United States)

    Anjum, Sadiya; Gupta, Amlan; Sharma, Deepika; Gautam, Deepti; Bhan, Surya; Sharma, Anupama; Kapil, Arti; Gupta, Bhuvanesh

    2016-07-01

    This study is aimed at the development of a composite material for wound dressing containing nanosilver nanohydrogels (nSnH) along with Aloe vera and curcumin that promote antimicrobial nature, wound healing and infection control. Nanosliver nanohydrogels were synthesized by nanoemulsion polymerization of methacrylic acid (MAA) followed by subsequent crosslinking and silver reduction under irradiation. Both the polymerization and irradiation time had significant influence on the nanoparticle shape, size and its formation. Polyvinyl alcohol/polyethylene oxide/carboxymethyl cellulose matrix was used as gel system to blend with nSnH, A. vera, curcumin and coat it on the hydrolysed PET fabric to develop antimicrobial dressings. The cumulative release of silver from the dressing was found to be ~42% of the total loading after 48h. The antimicrobial activity of the dressings was studied against both Staphylococcus aureus and Escherichia coli. In vivo wound healing studies were carried out over a period of 16d on full-thickness skin wounds created on Swiss albino mice. Fast healing was observed in Gel/nSnH/Aloe treated wounds with minimum scarring, as compared to other groups. The histological studies showed A. vera based dressings to be the most optimum one. These results suggest that nSnH along with A. vera based dressing material could be promising candidates for wound dressings.

  9. Antibacterial Effect of Aloe Vera Gel against Oral Pathogens: An In-vitro Study

    Science.gov (United States)

    Jain, Supreet; Rathod, Nirav; Sur, Jaideep; Laheji, Afshan; Gupta, Naveen; Agrawal, Priyanka; Prasad, Swati

    2016-01-01

    Introduction Natural herbal remedies have shown promising anti-microbial property and fewer side effects compared to synthetic anti-microbial therapy. Aloe Vera is a medicinal plant used for management of various infections since ancient times as it has anti-inflammatory, anti-microbial, and immune-boosting properties. Aim The aim of the present study was to determine the anti-microbial and inhibitory activities of various concentration of Aloe Vera Gel (AVG) against oral pathogenic bacteria. Materials and Methods Subgingival calculus and aspiration of periapical abscess and periodontal abscess was done in 20 patients and the sample transferred to thioglycolate broth, which was incubated in Mutans Sanguis agar, blood agar and cultured in anaerobic gas chamber. The colonies formed were identified further by gram staining methods and biochemical fermentation tests (IMViC). Each isolated colony of identified bacteria were cultured separately in Muller-Hilton broth and incubated at 37°C for 24 hours. Anti-microbial activity of the AVG was tested by the disc diffusion method and minimum inhibitory concentration was determined by broth micro-dilution method. Result Various staining and biochemical tests confirmed that the sample contained Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans), Clostridium bacilli (C. bacilli), Streptcoccus mutans (S. mutans) and Staphlococcus aureus (Staph. aureus). AVG showed anti-bacterial property at 100% and 50% concentration (‘t’ value = 7.504, p-value <0.001). At lower concentration there was no effect against the bacteria. At 100% AVG concentration, zone of inhibition measured was 6.9mm in A. actinomycetemcomitans, 6.3mm in C. bacilli, 6.8mm in S. mutans and 6.6mm in Staph. aureus. The standard drugs were also used to compare anti-bacterial property of AVG. Result showed that higher concentration (100%, 50%) of AVG has comparable zone of inhibition with Ofloxacin (5mcg) and Ciprofloxacin (30mcg). Conclusion AVG at

  10. Antifibrotic effect of aloe vera in viral infection-induced hepatic periportal fibrosis

    Institute of Scientific and Technical Information of China (English)

    Sahar K Hegazy; Mohamed El-Bedewy; Akira Yagi

    2012-01-01

    AIM:To investigate the anti-oxidative and anti-fibrotic effects of aloe vera in patients with liver fibrosis.METHODS:Aloe vera high molecular weight fractions (AHM) were processed by patented hyper-dry system in combination of freeze-dry technique with microwave and far infrared-ray radiation.Fifteen healthy volunteers as the control group and 40 patients were included.The patients were randomly subdivided into two equal groups:the conventional group was treated with placebo (starch),and AHM group was treated with 0.15 gm/d AHM,both for 12 consecutive weeks.The patients were investigated before and after treatment.Serum activity of aspartate aminotransferase (AST),alanine aminotransferase (ALT),alkaline phosphatase (ALP),hyaluronic acid (HA),transforming growth factor-β (TGF-β) and matrixmetalloproteinase-2 (MMP-2) were determined.The reduced glutathione (GSH) and malondialdehyde (MDA) levels in liver were assayed and the expression of hepatic α-smooth muscle actin (α-SMA)was identified by immunohistochemistry.RESULTS:At the start of the study,the hematoxylin and eosin staining revealed fibro-proliferated bile ductules,thick fibrous septa and dense inflammatory cellular infiltration in the patients before treatment.The use of AHM for 12 wk significantly ameliorated the fibrosis,inhibited the inflammation,and resulted in minimal infiltration and minimal fibrosis compared to the conventional group.The enzyme activities of the liver (ALT,AST and ALP) were attenuated after treatment in both groups,and the decrease in the AHM group was more significant as compared with the conventional group.Similar to the AST,the MDA levels were significantly higher before treatment,and were attenuated after treatment in both groups.In contrast,the hepatic glutathione content in the patients were decreased significantly in the AHM group compared to the controls.The serum levels of the fibrosis markers (HA,TGF-β and MMP-2) were also reduced significantly after treatment

  11. Aloe vera gel facilitates re-epithelialization of corneal alkali burn in normal and diabetic rats

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    Atiba A

    2015-10-01

    Full Text Available Ayman Atiba,1 Tamer Wasfy,2 Walied Abdo,3 Ahmed Ghoneim,2 Tarek Kamal,4 Mustafa Shukry5 1Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, 2Department of Ophthalmology, Faculty of Medicine, Tanta University, 3Department of Pathology, 4Department of Biochemistry, 5Department of Physiology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt Purpose: To investigate the efficacy of topical applied aloe vera (AV and to facilitate the repair of the standardized alkaline corneal ulcer in normal and diabetic rats.Materials and methods: The corneal alkali-burn injury model was established unilaterally in Wistar rats by filter paper saturated with 0.01 M NaOH contacting the eyes for 45 seconds. Rats were divided into four groups: normal control (NC, normal AV (NAV, diabetic control (DC, and diabetic AV (DAV. NAV and DAV groups were treated with AV gel eye drops four times daily, and NC and DC groups were treated with normal saline for 3 days. Corneal epithelial wound closure and degree of edema were recorded using slit lamp and optical coherence tomography at 0, 24, 48, and 72 hours postwounding. Histological examination was conducted to evaluate the degree of inflammation and the healing effect.Results: Corneal epithelial wound healing was better in the NAV group than in the NC group, and it was significantly higher in the DAV group than in the DC group (P<0.05. In comparison to the DC group, DAV treated with AV demonstrated a marked reduction in edema at 48 and 72 hours. Histologically, corneal re-epithelialization was complete and higher in DAV group than that in DC group; moreover, the inflammatory cells were increased in DC group than DAV group (P<0.05.Conclusion: This study demonstrated the efficacy of AV for enhanced corneal re-epithelialization, as well as reduced inflammatory response after alkali burn in rats; therefore, it could be useful as a

  12. A comparative study of the effects of topical application of Aloe vera, thyroid hormone and silver sulfadiazine on skin wounds in Wistar rats.

    Science.gov (United States)

    Tarameshloo, Mahsa; Norouzian, Mohsen; Zarein-Dolab, Saeed; Dadpay, Masoomeh; Gazor, Roohollah

    2012-03-01

    Many research studies report the healing effects of Aloe Vera, thyroid hormone cream and silver sulfadiazine. However, the effects of these therapeutic agents are not well understood and have not been compared in one study. This study aimed at investigating the effects of topical application of an Aloe vera gel, a thyroid hormone cream and a silver sulfadiazine cream on the healing of skin wounds surgically induced in Wistar rats for determining the treatment of choice. In a randomized controlled trial, twelve male rats, aged 120 days and with a mean weight of 250 to 300 g, were divided randomly into 5 groups based on drug treatments: Aloe vera gel (AV), thyroid hormone cream (TC), silver sulfadiazine 1% (S), vehicle (V) and control. To evaluate the efficacy of each treatment technique, a biomechanical approach was used to assess tensile stress after 14 days of treatment. Tensile stress was significantly improved in the Aloe vera gel group as compared with the other four groups (P≤0.05). While the other treatment options resulted in better healing than the control group, this difference was not significant. We conclude that Aloe vera topical application accelerated the healing process more than thyroid hormone, silver sulfadiazine and vehicle in surgically induced incisions in rats.

  13. Efficacy of Aloe Vera Supplementation on Prediabetes and Early Non-Treated Diabetic Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

    Science.gov (United States)

    Zhang, Yiyi; Liu, Wen; Liu, Dan; Zhao, Tieyun; Tian, Haoming

    2016-06-23

    The aim of this study was to evaluate evidence for the efficacy of aloe vera on managing prediabetes and early non-treated diabetes mellitus. We performed a systematic search of PubMed, Embase, and Cochrane Central Register of Controlled Trials until 28 January 2016. A total of five randomized controlled trials (RCTs) involving 415 participants were included. Compared with the controls, aloe vera supplementation significantly reduced the concentrations of fasting blood glucose (FBG) (p = 0.02; weighed mean difference [WMD]: -30.05 mg/dL; 95% confidence interval [CI]: -54.87 to -5.23 mg/dL), glycosylated hemoglobin A1c (HbA1c) (p Aloe vera was superior to placebo in increasing serum high density lipoprotein-cholesterol (HDL-C) levels (p = 0.04). Only one adverse event was reported. The evidence from RCTs showed that aloe vera might effectively reduce the levels of FBG, HbA1c, triglyceride, TC and LDL-C, and increase the levels of HDL-C on prediabetes and early non-treated diabetic patients. Limited evidence exists about the safety of aloe vera. Given the small number and poor quality of RCTs included in the meta-analysis, these results are inconclusive. A large-scale, well-designed RCT is needed to further address this issue.

  14. Efficacy of Aloe Vera Supplementation on Prediabetes and Early Non-Treated Diabetic Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

    Science.gov (United States)

    Zhang, Yiyi; Liu, Wen; Liu, Dan; Zhao, Tieyun; Tian, Haoming

    2016-01-01

    The aim of this study was to evaluate evidence for the efficacy of aloe vera on managing prediabetes and early non-treated diabetes mellitus. We performed a systematic search of PubMed, Embase, and Cochrane Central Register of Controlled Trials until 28 January 2016. A total of five randomized controlled trials (RCTs) involving 415 participants were included. Compared with the controls, aloe vera supplementation significantly reduced the concentrations of fasting blood glucose (FBG) (p = 0.02; weighed mean difference [WMD]: −30.05 mg/dL; 95% confidence interval [CI]: −54.87 to −5.23 mg/dL), glycosylated hemoglobin A1c (HbA1c) (p Aloe vera was superior to placebo in increasing serum high density lipoprotein-cholesterol (HDL-C) levels (p = 0.04). Only one adverse event was reported. The evidence from RCTs showed that aloe vera might effectively reduce the levels of FBG, HbA1c, triglyceride, TC and LDL-C, and increase the levels of HDL-C on prediabetes and early non-treated diabetic patients. Limited evidence exists about the safety of aloe vera. Given the small number and poor quality of RCTs included in the meta-analysis, these results are inconclusive. A large-scale, well-designed RCT is needed to further address this issue. PMID:27347994

  15. Crude Aloe vera Gel Shows Antioxidant Propensities and Inhibits Pancreatic Lipase and Glucose Movement In Vitro

    Directory of Open Access Journals (Sweden)

    Urmeela Taukoorah

    2016-01-01

    Full Text Available Aloe vera gel (AVG is traditionally used in the management of diabetes, obesity, and infectious diseases. The present study aimed to investigate the inhibitory potential of AVG against α-amylase, α-glucosidase, and pancreatic lipase activity in vitro. Enzyme kinetic studies using Michaelis-Menten (Km and Lineweaver-Burk equations were used to establish the type of inhibition. The antioxidant capacity of AVG was evaluated for its ferric reducing power, 2-diphenyl-2-picrylhydrazyl hydrate scavenging ability, nitric oxide scavenging power, and xanthine oxidase inhibitory activity. The glucose entrapment ability, antimicrobial activity, and total phenolic, flavonoid, tannin, and anthocyanin content were also determined. AVG showed a significantly higher percentage inhibition (85.56±0.91 of pancreatic lipase compared to Orlistat. AVG was found to increase the Michaelis-Menten constant and decreased the maximal velocity (Vmax of lipase, indicating mixed inhibition. AVG considerably inhibits glucose movement across dialysis tubes and was comparable to Arabic gum. AVG was ineffective against the tested microorganisms. Total phenolic and flavonoid contents were 66.06±1.14 (GAE/mg and 60.95±0.97 (RE/mg, respectively. AVG also showed interesting antioxidant properties. The biological activity observed in this study tends to validate some of the traditional claims of AVG as a functional food.

  16. In vivo macrophage activation in chickens with Acemannan, a complex carbohydrate extracted from Aloe vera.

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    Djeraba, A; Quere, P

    2000-05-01

    Acemannan (ACM 1), a beta-(1,4) -acetylated mannan isolated from Aloe vera, can be used as an effective adjuvant in vaccination against some avian viral diseases. Our results demonstrate a quick and lasting in vivo priming effect of ACM 1 on macrophage response after intramuscular inoculation in chickens (500 microg per 2-month-old bird). In response to IFN-gamma in vitro, monocytes from ACM 1-treated chickens exhibited a strong enhancement of NO production from 3 to 9 days p.i., but a weaker effect on MHC II cell surface antigen expression on day 3 p.i. A stimulating effect of ACM 1 treatment was also observed on spontaneous and inducible NO production for splenocytes only on day 3 p.i. By that time, splenocytes exhibited a strong higher capacity to proliferate in response to the T cell-mitogen PHA. At the same time, the in vivo capacity to produce NO, measured by the (NO(-)(2)+NO(-)(3)) serum level after intravenous LPS injection, increased greatly from 3 to 9 days p.i. In conclusion, ACM 1 was able efficiently and durably to increase the activation capacity of macrophages from the systemic immune compartment (in particular from the blood and spleen after an intramuscular injection) in chickens, especially for NO production. These findings provide a better understanding of the adjuvant activity of ACM 1 for viral and tumoral diseases.

  17. Deacetylation affects the physical properties and bioactivity of acemannan, an extracted polysaccharide from Aloe vera.

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    Chokboribal, Jaroenporn; Tachaboonyakiat, Wanpen; Sangvanich, Polkit; Ruangpornvisuti, Vithaya; Jettanacheawchankit, Suwimon; Thunyakitpisal, Pasutha

    2015-11-20

    Acemannan, an acetylated polymannose from Aloe vera, induces tissue repair. We investigated the role of acemannan's acetyl-groups on its physical and biological properties. Deacetylated acemannan (DeAcAM) was prepared and characterized. The physical properties and microscopic structure of DeAcAM were evaluated using water solubility, contact angle, X-ray diffraction, and scanning-electron microscopy. The activity of DeAcAM on cell proliferation and gene expression were assessed. Acemannan and DeAcAM structures were simulated and the acemannan tetramer diad and its completely deacetylated structure were also determined. Increased acemannan deacetylation reduced its water solubility and hydrophilicity. Complete deacetylation altered acemannan's conformation to a partial crystal structure. The bioactivity of acemannan was reduced corresponding to its deacetylation. Acemannan induced cell proliferation, and VEGF and Collagen I expression; however, 100% DeAcAM did not. The simulated structures of the acemannan diad and the completely deacetylated diad were different. We conclude acetyl-groups affect acemannan's structure and physical/biological properties.

  18. Development of Cells for Generation of Potential Difference from Aloe Vera

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    Yogendra B. Gandole

    2016-01-01

    Full Text Available Today’s need of this modern world is to introduce the renewable, sustainable, pollution free and an efficient energy sources. In this paper, some fundamental investigations are demonstrated for generating an electrical energy from living plants like Aloe Vera. The energy is generated by embedding the electrodes and cells into the plant to allow flow of ions using redox reaction. Here multiple tests have been conducted using different type of electrodes, cells and plants as an attempt to determine the characteristics of the generating system. Though the research work in this field is in infancy, we were able to produce the potential difference of about 1.221V using single pair of electrode and cell. Such hypothesis has been tested at different time of the day and different seasons of the year. Also, lots of new types of materials has been tested and used in combination to give better performance for the development of such a green energy. In future, this green energy could be used for low power electrical and electronic appliances for their operation

  19. Electrotonic potentials in Aloe vera L.: Effects of intercellular and external electrodes arrangement.

    Science.gov (United States)

    Volkov, Alexander G; Nyasani, Eunice K; Tuckett, Clayton; Scott, Jessenia M; Jackson, Mariah M Z; Greeman, Esther A; Greenidge, Ariane S; Cohen, Devin O; Volkova, Maia I; Shtessel, Yuri B

    2017-02-01

    Electrostimulation of plants can induce plant movements, activation of ion channels, ion transport, gene expression, enzymatic systems activation, electrical signaling, plant-cell damage, enhanced wound healing, and influence plant growth. Here we found that electrical networks in plant tissues have electrical differentiators. The amplitude of electrical responses decreases along a leaf and increases by decreasing the distance between polarizing Pt-electrodes. Intercellular Ag/AgCl electrodes inserted in a leaf and extracellular Ag/AgCl electrodes attached to the leaf surface were used to detect the electrotonic potential propagation along a leaf of Aloe vera. There is a difference in duration and amplitude of electrical potentials measured by electrodes inserted in a leaf and those attached to a leaf's surface. If the external reference electrode is located in the soil near the root, it changes the amplitude and duration of electrotonic potentials due to existence of additional resistance, capacitance, ion channels and ion pumps in the root. The information gained from this study can be used to elucidate extracellular and intercellular communication in the form of electrical signals within plants.

  20. Aloe vera affects changes induced in pulmonary tissue of mice caused by cigarette smoke inhalation.

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    Koul, Ashwani; Bala, Shashi; Yasmeen; Arora, Neha

    2015-09-01

    This study was undertaken to determine the influence of Aloe vera (AV) on changes induced in pulmonary tissue of cigarette smoke (CS) inhaling mice. CS inhalation for 4 weeks caused pulmonary damage as evident by histoarchitectural alterations and enhanced serum and tissue lactate dehydrogenase (LDH) activities. CS inhalation also led to increased mucin production as revealed by mucicarmine and Alcian Blue-Periodic Acid Schiff (AB-PAS) staining. Studies on bronchoalveolar lavage fluid (balf) of CS exposed animals revealed structural changes in phospholipids and increase in surface tension when compared with control counterparts. These changes were accompanied by enhanced nitric oxide (NO) levels, citrulline levels, peroxidative damage, and differential modulation of antioxidant defense system. AV administration (seven weeks, 500 mg/kg b.w. daily) to CS inhaling mice led to modulation of CS induced pulmonary changes as revealed by lesser degree of histoarchitectural alterations, lesser mucin production, decreased NO levels, citrulline levels, peroxidative damage, and serum LDH activity. AV treatment to CS inhaling mice was associated with varying response to antioxidant defense system, however balf of CS + AV treated animals did not exhibit appreciable changes when compared with that of CS exposed animals. These observations suggest that AV has the potential to modulate CS induced changes in the pulmonary tissue which could have implications in management of CS associated pulmonary diseases, however, further investigations are required to explore its complete mechanism of action.

  1. Use of Aloe vera-based extender for chilling and freezing collared peccary (Pecari tajacu) semen.

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    Souza, A L P; Lima, G L; Peixoto, G C X; Silva, A M; Oliveira, M F; Silva, A R

    2016-05-01

    As an alternative for the conservation of collared peccary semen, this research aims at evaluating the use of Aloe vera (AV) extract as a cryoprotectant for semen chilling and freezing. Five ejaculates were divided in two aliquots that were diluted in Tris plus egg yolk (EY; 20%) or AV extract (20%) and chilled at 5 °C. In both treatments, an adequate semen conservation was achieved and values closer to 40% motile sperm with viability and osmotic response ranging from 20% to 40%, and normal morphology of 80% were found after 36 hours of storage. Moreover, 12 other ejaculates were diluted in Tris plus EY (20%) or AV extract (5, 10, or 20%) and glycerol (3%). Samples were frozen in liquid nitrogen and thawed after 1 week. After thawing, all the treatments containing EY or AV provided similar values for sperm morphology, viability, osmotic response, membrane integrity, sperm motility, amplitude of lateral head, beat cross frequency, and rapid, low, and static subpopulations, but the highest values for straightness and the lowest values for curvilinear velocity were found using 20% AV (P < 0.05). In conclusion, we found that AV extract at a 20% concentration could be used as an alternative substitute to EY in the formulation of Tris extenders for collared peccaries' semen chilling or freezing.

  2. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

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    Yun Sun Lee

    Full Text Available Aloe vera (Asphodeloideae is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  3. Enhancement of anti-inflammatory activity of Aloe vera adventitious root extracts through the alteration of primary and secondary metabolites via salicylic acid elicitation.

    Science.gov (United States)

    Lee, Yun Sun; Ju, Hyun Kyoung; Kim, Yeon Jeong; Lim, Tae-Gyu; Uddin, Md Romij; Kim, Yeon Bok; Baek, Jin Hong; Kwon, Sung Won; Lee, Ki Won; Seo, Hak Soo; Park, Sang Un; Yang, Tae-Jin

    2013-01-01

    Aloe vera (Asphodeloideae) is a medicinal plant in which useful secondary metabolites are plentiful. Among the representative secondary metabolites of Aloe vera are the anthraquinones including aloe emodin and chrysophanol, which are tricyclic aromatic quinones synthesized via a plant-specific type III polyketide biosynthesis pathway. However, it is not yet clear which cellular responses can induce the pathway, leading to production of tricyclic aromatic quinones. In this study, we examined the effect of endogenous elicitors on the type III polyketide biosynthesis pathway and identified the metabolic changes induced in elicitor-treated Aloe vera adventitious roots. Salicylic acid, methyl jasmonate, and ethephon were used to treat Aloe vera adventitious roots cultured on MS liquid media with 0.3 mg/L IBA for 35 days. Aloe emodin and chrysophanol were remarkably increased by the SA treatment, more than 10-11 and 5-13 fold as compared with untreated control, respectively. Ultra-performance liquid chromatography-electrospray ionization mass spectrometry analysis identified a total of 37 SA-induced compounds, including aloe emodin and chrysophanol, and 3 of the compounds were tentatively identified as tricyclic aromatic quinones. Transcript accumulation analysis of polyketide synthase genes and gas chromatography mass spectrometry showed that these secondary metabolic changes resulted from increased expression of octaketide synthase genes and decreases in malonyl-CoA, which is the precursor for the tricyclic aromatic quinone biosynthesis pathway. In addition, anti-inflammatory activity was enhanced in extracts of SA-treated adventitious roots. Our results suggest that SA has an important role in activation of the plant specific-type III polyketide biosynthetic pathway, and therefore that the efficacy of Aloe vera as medicinal agent can be improved through SA treatment.

  4. Reduction of nectarine decay caused by Rhizopus stolonifer, Botrytis cinerea and Penicillium digitatum with Aloe vera gel alone or with the addition of thymol.

    Science.gov (United States)

    Navarro, Diana; Díaz-Mula, Huertas M; Guillén, Fabián; Zapata, Pedro J; Castillo, Salvador; Serrano, María; Valero, Daniel; Martínez-Romero, Domingo

    2011-12-02

    Two nectarine cultivars ('Flavela' and 'Flanoba') were treated with Aloe vera gel alone, or with the addition of thymol, and then inoculated with Rhizopus stolonifer, Botrytis cinerea and Penicillium digitatum. Both treatments were effective in reducing the decay incidence caused by the 3 fungi species, although the addition of thymol did not generally improve the efficacy of Aloe vera gel on reducing the infection damage. The coatings were clearly effective in reducing the postharvest ripening process of both nectarine cultivars manifested by a delay in ethylene production and respiration rate, weight loss and softening. Interestingly, these coatings showed effectiveness on reducing decay development in inoculated fruits and thus Aloe vera could be considered as natural antifungal compound and might serve as alternative of synthetic fungicides.

  5. Combination of Aloe vera and xenograft induction on decreasing of NF-kb of tooth extraction socket preservation in Cavia cobaya

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    Utari Kresnoadi

    2014-03-01

    Full Text Available Background: Tooth extraction can naturally cause inflammation triggering osteoclast proliferation and alveolar bone resorption. Preservation of the tooth extraction sockets is needed for patients in order to reduce alveolar bone resorption risks. Aloe vera is known to have anthraquinones components, namely Aloin, Aloe emedin, and barbaloin, considered as anti-inflammation. Therefore, to overcome the inflammation, the role of NF-kb is very significant to decrease nuclear factor kappa b (NF-kb. As a result, inflammation risks will be decreased. Purpose: The study was aimed to determine the induction effect of combination of Aloe vera and XCB into tooth extraction sockets to reduce inflammation by reducing NF-kb expression, osteoclasts and osteoblasts. Methods: Forty-eight Cavia cobaya were divided into eight groups, each group consisted of six animals. The mandibular incisors of those Cavia cobaya were extracted and induced with either PEG, XCB, Aloe vera, or the combination of Aloe vera + XCB. Those animals were sacrificed on day 7 and day 30 after the extraction. Then immunohistochemical and histopathology examinations were conducted to observe NF-kb expression, osteoblasts and osteoclasts. Results: It was known that in group induced with the combination of Aloe vera and xenograft concelous bovine, the growth of osteoblasts was high, while NF-kb expression and osteoclasts reduced. Conclusion: It can be concluded that the induction of the combination of Aloe vera and XCB into the tooth extraction sockets can reduce NF-kb expression and osteoclast, as a result, alveolar bone resorption risks decrease, and osteoblast increase.Latar belakang: Trauma mekanis akibat pencabutan gigi asli menyebabkan keradangan. Keradangan memicu proliferasi osteoklas sehingga menyebabkan resorpsi tulang alveolararis. Pada pembuatan gigi tiruan, resorpsi tulang alveolar yang terjadi, sangat tidak diinginkan, sebab resorpsi tulang alveolar mengurangi keberhasilan

  6. Perbedaan Efek Pemberian Topikal Gel Lidah Buaya (Aloe vera L. Dengan Solusio Povidone Iodine Terhadap Penyembuhan Luka Sayat Pada Kulit Mencit (Mus musculus

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    Nur Atik

    2009-06-01

    Full Text Available Normal wound healing is a complex and dynamic process. Wound healing process can accelerate, with chemical treatment or natural. The chemical treatment often used in healing process is povidone iodine. For natural treatment, topical application of Aloe vera gel may accelerate the full-thickness wound healing process. The purpose of this research was to evaluate the differences between topical application of povidone iodine and Aloe vera gel for skin wound healing in mice. This study used 18 mice that were divided into three groups. First group was the wounded (control group, the second group was wounded – Aloe vera group, the third group was wounded - povidone iodine group. Full-thickness skin wound were created on the dorsal area of mice in each group. The control group were not given anything, while the second group were given Aloe vera gel twice a day, and the third group were given povidone iodine solution twice a day. At the fifth day, all mice were sacrificed for histologic evaluation and VEGF A expression. Data was obtained by microscopic observation of the wounded skin, based on quantitative parameter: epithelial thickness, total fibroblast, total blood vessels, and VEGF A expression. Then the data was statistically analyzed by using independent samples T test, ANOVA, and Chi square. The result demonstrated that the sum of epithelial thickness, fibroblast, blood vessels, and VEGF A expression in the Aloe vera group is higher than in povidone iodine group. Statistic evaluation showed that there were significant differences between the two groups (p < 0.05, with 95% confidence interval. Based on this result, it can be concluded that the topical administration of Aloe vera gel twice a day is better than povidone iodine with parameter epithelial thickness, total fibroblass, total blood vessels and VEGF A expression.

  7. Comparison of the Antibacterial Effect of Sodium Hypochlorite and Aloe Vera Solutions as Root Canal Irrigants in Human Extracted Teeth Contaminated with Enterococcus Faecalis

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    Sahebi S.

    2013-10-01

    Full Text Available Statement of Problem: The main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. Sodium hypochlorite has excellent antibacterial properties, but also some negative features. Purpose: The aim of the present study is to compare the antimicrobial effect of Aloe Vera so-lution with sodium hypochlorite on E.faecalis in the root canals of human extracted teeth. Materials and Method: Sixty human extracted single rooted teeth were selected for this in vitro study. The teeth recruited in this study had no cracks, internal resorption, external resorption and calcification. Enterococcus faecalis was injected in the root canals of all teeth. The teeth were then divided into three groups randomly. Each group consisted of 20 teeth that were all rinsed with one of the following solutions: sodium hypochlorite 2.5%, Aloe vera and normal saline. Subsequent to rinsing, root canals of all teeth were sampled. The samples were cultured and growth of the bacteria was assessed after 48 hours. The number of colonies of the bacteria was then counted. Results: The difference between the inhibitory effect of Aloe vera and normal saline on E.faecalis was not significant according to independent t-test (p= 0.966. The inhibitory effect of sodium hypochlorite on E.faecalis was much greater than that of Aloe vera and normal saline (p< 0.001. Conclusion: Aloe vera solution is not recommended as a root canal irrigator, but future studies are suggested to investigate the antibacterial effect of Aloe vera with longer duration of exposure and as an intra canal medicament.

  8. Comparison of the Antibacterial Effect of Sodium Hypochlorite and Aloe Vera Solutions as Root Canal Irrigants in Human Extracted Teeth Contaminated with Enterococcus Faecalis

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    Sahebi S.

    2014-03-01

    Full Text Available Statement of Problem: The main purpose of a root canal treatment is to eliminate the bacteria and their products from the pulp space. Sodium hypochlorite has excellent antibacterial properties but also some negative features. Purpose: The aim of the present study is to compare the antimicrobial effect of Aloe Vera solution with sodium hypochlorite on E.faecalis in the root canals of human extracted teeth. Materials and Method: Sixty human extracted single rooted teeth were selected for this in vitro study. Only teeth without conditions such as cracks, internal resorption, external resorption and calcification were included in the study. Enterococcus faecalis was injected in the root canals of all teeth. The teeth were then divided into three groups randomly. Each group consisted of 20 teeth that were all rinsed with one of the following solutions: sodium hypochlorite 2.5%, Aloe vera and normal saline. Subse-quent to rinsing, root canals of all teeth were sampled. The samples were cultured and growth of the bacteria was assessed after 48 hours. The number colonies of the bacte-ria were then counted. Results: The difference between the inhibitory effect of Aloe vera and normal saline on E.faecalis was not significant according to independent T-test (p= 0.966. The in-hibitory effect of sodium hypochlorite on E.faecalis was much greater than that of Aloe vera and normal saline (p< 0.001. Conclusion: Aloe vera solution is not recommended as a root canal irrigator but it is suggested for the future studies to investigate the antibacterial effect of Aloe vera for longer durations and as an intracanal medicament.

  9. Mosquito larvicidal activity of Aloe vera (Family: Liliaceae) leaf extract and Bacillus sphaericus, against Chikungunya vector, Aedes aegypti

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    Subramaniam, Jayapal; Kovendan, Kalimuthu; Mahesh Kumar, Palanisamy; Murugan, Kadarkarai; Walton, William

    2012-01-01

    The bio-efficacy of Aloe vera leaf extract and bacterial insecticide, Bacillus sphaericus larvicidal activity was assessed against the first to fourth instars larvae of Aedes aegypti, under the laboratory conditions. The plant material was shade dried at room temperature and powdered coarsely. A. vera and B. sphaericus show varied degrees of larvicidal activity against various instars larvae of A. aegypti. The LC50 of A. vera against the first to fourth instars larvae were 162.74, 201.43, 253.30 and 300.05 ppm and the LC90 442.98, 518.86, 563.18 and 612.96 ppm, respectively. B. sphaericus against the first to fourth instars larvae the LC50 values were 68.21, 79.13, 93.48, and 107.05 ppm and the LC90 values 149.15, 164.67, 183.84, and 201.09 ppm, respectively. However, the combined treatment of A. vera + B. sphaericus (1:2) material shows highest larvicidal activity of the LC50 values 54.80, 63.11, 74.66 and 95.10 ppm; The LC90 values of 145.29, 160.14, 179.74 and 209.98 ppm, against A. aegypti in all the tested concentrations than the individuals and clearly established that there is a substantial amount of synergist act. The present investigation clearly exhibits that both A. vera and B. sphaericus materials could serve as a potential larvicidal agent. Since, A. aegypti is a container breeder vector mosquito this user and eco-friendly and low-cost vector control strategy could be a viable solution to the existing dengue disease burden. Therefore, this study provides first report on the mosquito larvicidal activity the combined effect of A. vera leaf extract and B. sphaericus against as target species of A. aegypti. PMID:23961212

  10. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

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    Patcharaporn Tippayawat

    2016-10-01

    Full Text Available Background There is worldwide interest in silver nanoparticles (AgNPs synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV with antibacterial activity. Methods AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively

  11. Green synthesis of silver nanoparticles in aloe vera plant extract prepared by a hydrothermal method and their synergistic antibacterial activity

    Science.gov (United States)

    Phromviyo, Nutthakritta; Boueroy, Parichart; Chompoosor, Apiwat

    2016-01-01

    Background There is worldwide interest in silver nanoparticles (AgNPs) synthesized by various chemical reactions for use in applications exploiting their antibacterial activity, even though these processes exhibit a broad range of toxicity in vertebrates and invertebrates alike. To avoid the chemical toxicity, biosynthesis (green synthesis) of metal nanoparticles is proposed as a cost-effective and environmental friendly alternative. Aloe vera leaf extract is a medicinal agent with multiple properties including an antibacterial effect. Moreover the constituents of aloe vera leaves include lignin, hemicellulose, and pectins which can be used in the reduction of silver ions to produce as AgNPs@aloe vera (AgNPs@AV) with antibacterial activity. Methods AgNPs were prepared by an eco-friendly hydrothermal method using an aloe vera plant extract solution as both a reducing and stabilizing agent. AgNPs@AV were characterized using XRD and SEM. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. MIC and MBC were used to correlate the concentration of AgNPs@AV its bactericidal effect. SEM was used to investigate bacterial inactivation. Then the toxicity with human cells was investigated using an MTT assay. Results The synthesized AgNPs were crystalline with sizes of 70.70 ± 22-192.02 ± 53 nm as revealed using XRD and SEM. The sizes of AgNPs can be varied through alteration of times and temperatures used in their synthesis. These AgNPs were investigated for potential use as an antibacterial agent to inhibit pathogenic bacteria. Their antibacterial activity was tested on S. epidermidis and P. aeruginosa. The results showed that AgNPs had a high antibacterial which depended on their synthesis conditions, particularly when processed at 100 oC for 6 h and 200 oC for 12 h. The cytotoxicity of AgNPs was determined using human PBMCs revealing no obvious cytotoxicity. These results indicated that AgNPs@AV can be effectively utilized in

  12. Formulation and evaluation of sustained release matrix tablets of pioglitazone hydrochloride using processed Aloe vera mucilage as release modifier

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    Manoj Choudhary

    2015-01-01

    Full Text Available Background: Natural gums and mucilage which hydrates and swells on contact with aqueous media are used as additives in the formulation of hydrophilic drug delivery system. Aim: The purpose of this study was to develop a new monolithic matrix system for complete delivery of Pioglitazone hydrochloride (HCl, in a zero-order manner over an extended time period using processed Aloe vera gel mucilage (PAG as a release modifier. Materials and Methods: The matrices were prepared by dry blending of selected ratios of polymer and ingredients using direct compression technique. Physicochemical properties of dried powdered mucilage of A. vera were studied. Various formulations of pioglitazone HCl and A. vera mucilage were prepared using different drug: Polymer ratios viz., 1:1, 1:2, 1:3, 1:4, 1:5 for PAG by direct compression technique. Results: The formulated matrix tablets were found to have better uniformity of weight and drug content with low statistical deviation. The swelling behavior and in vitro release rate characteristics were also studied. Conclusion: The study proved that the dried A. vera mucilage can be used as a matrix forming material for controlled release of Pioglitazone HCl matrix tablets.

  13. Anti-arthritic effects of Musa paradisiaca sapientum L. and Aloe vera L. in adjuvant-induced arthritic rats

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    ENDANG DARMAWAN

    2006-07-01

    Full Text Available Anti-arthritic effects of Musa paradisiaca sapientum L. and Aloe vera L. on the index responses of arthritic were investigated using adjuvant-induced arthritic rats. M. paradisiaca sapientum and A. vera pulp were blended into juice then the juices were administered orally on 17-30th day. To evaluate anti-arthritic effect of the juice, an arthritic rat model was developed using Complete Freund Adjuvant on 1-16th day. In addition, the research was divided into groups (n=5 including normal control groups, positive control groups, negative control groups, 3 groups were treated by M. paradisiaca juice at 50, 100, 200 mg/kgBW doses and the other groups were treated by A. vera juices at 1, 2 and 4 mg/kgBW doses. The indexes of arthritic were determined on 17th and 31st days then analyzed using ANOVA test and t test (p<0.05. The result showed that the indexes of arthritic rats were evidently decreased by either M. sapientum or A. vera juice. Moreover, indexes of arthritic to be closely restored to the normal levels through M. paradisiaca treatment at 50 mg/kg BW dose. In conclusion, both of juice is found to be effective in decreasing the inflammatory response and arthritic symptoms in adjuvant-induced arthritic rats.

  14. Antibacterial effect of triantibiotic mixture, chlorhexidine gel, and two natural materials Propolis and Aloe vera against Enterococcus faecalis: An ex vivo study

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    Leila Bazvand

    2014-01-01

    Full Text Available Background: The aim of this ex vivo study was to compare the antimicrobial effect of triantibiotic paste, 0.2% chlorhexidine gel, Propolis and Aloe vera on Enterococcus faecalis in deep dentin. Materials and Methods: Ninety fresh extracted single-rooted teeth were used in a dentin block model. Seventy-five teeth were infected with E. faecalis and divided into four experimental groups (n = 15. Experimental groups were treated with triantibiotic mixture with distilled water, 0.2% chlorhexidine gel, 70% ethanol + Propolis and Aloe vera. Fifteen teeth treated with distilled water as the positive control and 15 samples, free of bacterial contamination, were considered as the negative control. Gates-Glidden drill #4 was used for removal of surface dentin and Gates-Glidden drill #5 was used to collect samples of deep dentin. The samples were prepared and colony-forming units were counted. Data were analyzed by one-way ANOVA and post hoc Tukey tests. Statistical significance was defined at P 0.05. Aloe vera had antibacterial effects on E. faecalis, but in comparison with other medicaments, it was less effective (P < 0.05. Conclusion: This experimental study showed that triantibiotic mixture, 0.2% chlorhexidine gel, Propolis and Aleo vera were relatively effective against E. faecalis. All the intracanal medicements had similar effects on E. faecalis in deep dentin except for Aloe vera.

  15. Measurement of the transmittance of edible films of aloe vera (barbadensis miller) and cassava starch using optical fibers trifurcated

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    Díaz, L.; Reales, J.; Torres, C.

    2017-01-01

    In Colombia, especially in the Atlantic Coast it is produced and marketed the costeño cheese, an indigenous product of the gastronomy of this region, but the prolonged exposure of this product to the environment leads to microbial contamination and non-enzymatic rancidity. For this reason the transmittance of an edible coating based in aloe vera gel and cassava starch to preserve costeño cheese was evaluated using trifurcated optical fibers. The results become a tool for the selection of treatments in making edible films and their subsequent use in coatings for various types of food products.

  16. Dietary aloe vera gel powder and extract inhibit azoxymethane- induced colorectal aberrant crypt foci in mice fed a high- fat diet.

    Science.gov (United States)

    Chihara, Takeshi; Shimpo, Kan; Kaneko, Takaaki; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru; Tanaka, Miyuki; Yamada, Muneo; Abe, Fumiaki

    2015-01-01

    Aloe vera gel exhibits protective effects against insulin resistance as well as lipid-lowering and anti-diabetic effects. The anti-diabetic compounds in this gel were identified as Aloe-sterols. Aloe vera gel extract (AVGE) containing Aloe-sterols has recently been produced using a new procedure. We previously reported that AVGE reduced large-sized intestinal polyps in Apc-deficient Min mice fed a high fat diet (HFD), suggesting that Aloe vera gel may protect against colorectal cancer. In the present study, we examined the effects of Aloe vera gel powder (AVGP) and AVGE on azoxymethane-induced colorectal preneoplastic aberrant crypt foci (ACF) in mice fed a HFD. Male C57BL/6J mice were given a normal diet (ND), HFD, HFD containing 0.5% carboxymethyl cellulose solution, which was used as a solvent for AVGE (HFDC), HFD containing 3% or 1% AVGP, and HFDC containing 0.0125% (H-) or 0.00375% (L-) AVGE. The number of ACF was significantly lower in mice given 3% AVGP and H-AVGE than in those given HFD or HFDC alone. Moreover, 3% AVGP, H-AVGE and L-AVGE significantly decreased the mean Ki-67 labeling index, assessed as a measure of cell proliferation in the colonic mucosa. In addition, hepatic phase II enzyme glutathione S-transferase mRNA levels were higher in the H-AVGE group than in the HFDC group. These results suggest that both AVGP and AVGE may have chemopreventive effects on colorectal carcinogenesis under the HFD condition. Furthermore, the concentration of Aloe-sterols was similar between 3% AVGP and H-AVGE, suggesting that Aloe-sterols were the main active ingredients in this experiment.

  17. Optimality in Microwave-Assisted Drying of Aloe Vera ( Aloe barbadensis Miller) Gel using Response Surface Methodology and Artificial Neural Network Modeling

    Science.gov (United States)

    Das, Chandan; Das, Arijit; Kumar Golder, Animes

    2016-10-01

    The present work illustrates the Microwave-Assisted Drying (MWAD) characteristic of aloe vera gel combined with process optimization and artificial neural network modeling. The influence of microwave power (160-480 W), gel quantity (4-8 g) and drying time (1-9 min) on the moisture ratio was investigated. The drying of aloe gel exhibited typical diffusion-controlled characteristics with a predominant interaction between input power and drying time. Falling rate period was observed for the entire MWAD of aloe gel. Face-centered Central Composite Design (FCCD) developed a regression model to evaluate their effects on moisture ratio. The optimal MWAD conditions were established as microwave power of 227.9 W, sample amount of 4.47 g and 5.78 min drying time corresponding to the moisture ratio of 0.15. A computer-stimulated Artificial Neural Network (ANN) model was generated for mapping between process variables and the desired response. `Levenberg-Marquardt Back Propagation' algorithm with 3-5-1 architect gave the best prediction, and it showed a clear superiority over FCCD.

  18. Optimality in Microwave-Assisted Drying of Aloe Vera (Aloe barbadensis Miller) Gel using Response Surface Methodology and Artificial Neural Network Modeling

    Science.gov (United States)

    Das, Chandan; Das, Arijit; Kumar Golder, Animes

    2016-07-01

    The present work illustrates the Microwave-Assisted Drying (MWAD) characteristic of aloe vera gel combined with process optimization and artificial neural network modeling. The influence of microwave power (160-480 W), gel quantity (4-8 g) and drying time (1-9 min) on the moisture ratio was investigated. The drying of aloe gel exhibited typical diffusion-controlled characteristics with a predominant interaction between input power and drying time. Falling rate period was observed for the entire MWAD of aloe gel. Face-centered Central Composite Design (FCCD) developed a regression model to evaluate their effects on moisture ratio. The optimal MWAD conditions were established as microwave power of 227.9 W, sample amount of 4.47 g and 5.78 min drying time corresponding to the moisture ratio of 0.15. A computer-stimulated Artificial Neural Network (ANN) model was generated for mapping between process variables and the desired response. `Levenberg-Marquardt Back Propagation' algorithm with 3-5-1 architect gave the best prediction, and it showed a clear superiority over FCCD.

  19. Isolation and characterization of structural components of Aloe vera L. leaf pulp.

    Science.gov (United States)

    Ni, Y; Turner, D; Yates, K M; Tizard, I

    2004-12-20

    The clear pulp, also known as inner gel, of Aloe vera L. leaf is widely used in various medical, cosmetic and nutraceutical applications. Many beneficial effects of this plant have been attributed to the polysaccharides present in the pulp. However, discrepancies exist regarding the composition of pulp polysaccharide species and an understanding of pulp structure in relation to its chemical composition has been lacking. Thus, we examined pulp structure, isolated structural components and determined their carbohydrate compositions along with analyzing a partially purified pulp-based product (Acemannan hydrogel) used to make Carrisyn hydrogel wound dressing. Light and electron microscopy showed that the pulp consisted of large clear mesophyll cells with a diameter as large as 1000 microm. These cells were composed of cell walls and cell membranes along with a very limited number of degenerated cellular organelles. No intact cellular organelles were found in mesophyll cells. Following disruption of pulp by homogenization, three components were isolated by sequential centrifugation. They were thin clear sheets, microparticles and a viscous liquid gel, which corresponded to cell wall, degenerated cellular organelles and liquid content of mesophyll cells based on morphological and chemical analysis. These three components accounted for 16.2% (+/-3.8), 0.70% (+/-0) and 83.1% of the pulp on a dry weight basis. The carbohydrate composition of each component was distinct; liquid gel contained mannan, microparticles contained galactose-rich polysaccharide(s) and cell walls contained an unusually high level of galacturonic acid (34%, w/w; Gal A). The same three components were also found in Acemannan Hydrogel with mannan as the predominant component. Thus, different pulp structural components are associated with different polysaccharides and thus may potentially be different functionally. These findings may help lay a basis for further studies and development of better

  20. Uso de cobertura com colágeno e aloe vera no tratamento de ferida isquêmica: estudo de caso El uso de colágeno y aloe vera en el tratamiento de la herida isquémica: estudio de caso Use of collagen and aloe vera in ischemic wound treatment: study case

    OpenAIRE

    2010-01-01

    O presente estudo relata o caso clínico de um paciente diabético e hipertenso, portador de ferida isquêmica, tratado com cobertura não convencional, à base de Aloe vera e colágeno. Faz parte de um projeto de pesquisa experimental, coordenado por professores e enfermeiros que atuam em projetos para o desenvolvimento de novas tecnologias para o tratamento de feridas. O caso em tela foi selecionado dentre os dos demais pacientes acompanhados. A coleta de dados foi efetuada através da anamnese e ...

  1. Utilization of plant bioactives as feed additives for poultry: The effect of Aloe vera gel and its extract on performance of broilers

    Directory of Open Access Journals (Sweden)

    A.P Sinurat

    2003-10-01

    Full Text Available Feed additives are commonly added in poultry feed as a growth promotant or to improve feed efficiency. The most common feed additive used is antibiotic at sub-therapheutic doses, although there is a controversy on its impact on human health. Previous results showed that Aloe vera gel could improve feed efficiency in broilers and an in vitro study showed that the extract have an antibacterial effect. Therefore, a further experiment was designed to study the response of broilers to Aloe vera gel or its extract as feed additives. Aloe vera was prepared in dry gel or chloroform-extract and included in the diet at levels of 0.25; 0.50 and 1.00 g/kg (equal to dry gel. Standard diets with or without antibiotic were also formulated as control and a commercial diet was included for comparison. The diets were fed to broilers from day old to 5 weeks. Each treatment has 9 replicates and 6chicks/replicate. Parameters observed were: feed consumption, weight gain and feed convertion ratios. Carcass yield, abdominal fat levels, relative weight of liver, gizzard, tractus digestivus and length of tractus digestivus were also measured at the end of feeding trial. The results showed that Aloe gel and its extract did not influence body weight gain and feed consumption of broilers significantly (P>0.05, but improved feed convertion slightly (3.50%. The response in this trial was similar as thosecommercial diet and diet added with antibiotic. There was no significant (P>0.05 effect of Aloe vera bioactives on carcass yield, abdominal fat level and relative weight of liver. However, Aloe vera gel and its extract tend to increase gizzard weight, gastro intestinal weight and length. The Aloe vera gel and its extract also reduced the total count of aerobic bacteria in the digesta of tractus digestivus. It is concluded that the Aloe vera gel improve feed efficiency in broilers by increasing the size of tractus digestivus and reducing the total count of aerobic bacteria in

  2. Effect of Aloe vera and clove powder supplementation on carcass characteristics, composition and serum enzymes of Japanese quails

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    Hujaz Tariq

    2015-05-01

    Full Text Available Aim: The aim was to study the effect of Aloe vera and clove powder supplementation on carcass characteristics, composition and serum enzymes of Japanese quails. Materials and Methods: The study was conducted on 120-day-old Japanese quails, which were randomly divided into four treatment groups viz. T1-control, T2-0.5% A. vera, T3-0.5% clove, and T4-(0.25% A. vera + 0.25% clove powder, each having three replications consisting of ten Japanese quails. The birds in control group (T1 were fed no supplement whereas in treatments T2-T4 birds were supplemented with A. vera leaf powder, clove, and mixture of both (powdered form at 0.5, 0.5, and 0.25+0.25% of feed, respectively. After 7 days of brooding, a feeding trial of 35 days was conducted. On 35th day of trial birds were sacrificed and dressing percentage, cut up parts, organ weight, processing losses and carcass meat composition of the breast, thigh, and drumstick were recorded. Blood samples were collected on the same day and serum was separated to evaluate serum enzymes alanine aminotransferase (ALT and aspartate aminotransferase (AST. Results: It showed significantly (p0.05 differences were observed in giblet weight, other cut up parts and composition of the breast, thigh, and drumstick muscles in all the treatment groups. Serum ALT and AST did not vary significantly among different treatment groups. Conclusion: A. vera and clove supplementation improved the dressing percentage and breast weight without adversely affecting the meat composition and serum enzymes. Thus, these can be used as a growth promoter in Japanese quails.

  3. Shape- and Size-Controlled Synthesis of Silver Nanoparticles Using Aloe vera Plant Extract and Their Antimicrobial Activity

    Science.gov (United States)

    Logaranjan, Kaliyaperumal; Raiza, Anasdass Jaculin; Gopinath, Subash C. B.; Chen, Yeng; Pandian, Kannaiyan

    2016-11-01

    Biogenic synthesis of silver nanoparticles (AgNP) was performed at room temperature using Aloe vera plant extract in the presence of ammoniacal silver nitrate as a metal salt precursor. The formation of AgNP was monitored by UV-visible spectroscopy at different time intervals. The shape and size of the synthesized particle were visualized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations. These results were confirmed by X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) analyses and further supported by surface-enhanced Raman spectroscopy/Raman scattering (SERS) study. UV-visible spectrum has shown a sharp peak at 420 nm and further evidenced by FTIR peak profile (at 1587.6, 1386.4, and 1076 cm-1 with corresponding compounds). The main band position with SERS was noticed at 1594 cm-1 (C-C stretching vibration). When samples were heated under microwave radiation, AgNP with octahedron shapes with 5-50 nm were found and this method can be one of the easier ways to synthesis anisotropic AgNP, in which the plant extract plays a vital role to regulate the size and shape of the nanoparticles. Enhanced antibacterial effects (two- to fourfold) were observed in the case of Aloe vera plant protected AgNP than the routinely synthesized antibiotic drugs.

  4. Enrichment of anti-ulcer activity of monoammonium glycerrhizin and Aloe vera gel powder through a novel drug delivery system

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    Arati N Ranade

    2014-01-01

    Full Text Available Usual treatment for Helicobacter pylori-induced peptic ulcer includes a 14-day "triple therapy" of two antibiotics and a proton pump inhibitor. However, the current therapy has side-effects like stomach upset, non-compliance, incomplete absorption of drug and antibiotic resistance. To overcome these limitations, there is a need to suggest an alternative therapy. The best possible alternative is to deliver herbal constituents. The purpose of the present study was to optimize the efficacy of herbal constituents by applying the concept of a novel drug delivery system. The present investigation is designed to deliver and retain two herbal constituents in the stomach for better action against gastric ulcers. The objective was to develop a bilayer floating tablet of monoammonium glycerrhizin and Aloe vera gel powder through rational combination of excipients to give the lowest possible lag time with maximum drug release in 7 h. Formulation OF2 containing hydroxy propyl methyl cellulose E5, crospovidone and effervescent agents in the ratio 1:2 gave 98% drug release with desired floating properties. Pharmacodynamic studies in rats showed that the combination of monoammonium glycerrhizin and Aloe vera gave 99% ulcer inhibition in comparison with 51% ulcer inhibition in the group administered with monoammonium glycerrhizin alone. X-ray studies in rabbits proved the gastroretention of the tablet for more than 6 h. This suggests relevance of NDDS in delivery of herbal constituents in the treatment of gastric ulcer.

  5. The use of 90% Aloe vera freeze drying as the modulator of collagen density in extraction socket of incicivus Cavia cobaya

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    Ester Arijani

    2008-06-01

    Full Text Available Wound healing is basically a complex process in which cellular and matrix act in concern to re-establish the integrity of injury tissues. This process can be simplified to be healing process consisted of haemostatic, inflammation, cell proliferation and tissue remodeling. The aimed of this research was to know the influence of freeze drying 90% Aloe vera application as mandible collagen density modulator in extraction socket of incisive Cavia cobaya. This research was done using Post Test Only Control Groups Design and Cavia cobaya as the sample. Six samples of each control group and 90% Aloe vera group applied to test each collagen density for three days and seven days. Then, the data was analyzed statistically using Mann Whitney with 5% significance rate. The result of the study indicates that administering 90% Aloe vera can accelerate the growth of collagen density in healing process of extraction socket. The conclusion is 90% Aloe vera can modulate the density of collageneous fiber in socket of extraction incicivus tooth wound of Cavia cobaya.

  6. Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na{sup 99m}TcO{sub 4}) in rats

    Energy Technology Data Exchange (ETDEWEB)

    Holanda, Cecilia Maria de Carvalho Xavier [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Dept. of Microbiology and Parasitology. Experimental Radiobiology and Antiparasitic Assays Lab.], e-mail: cechol@ufrnet.br; Costa, Monique Batista da; Silva, Natalia Chilinque Zambao da; Silva Junior, Mauricio Ferreira da; Barbosa, Vanessa Santos de Arruda; Silva, Roseane Pereira da [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil); Medeiros, Aldo da Cunha [Universidade Federal do Rio Grande do Norte (UFRN), Natal, RN (Brazil). Nucleus of Experimental Surgery

    2009-07-01

    Purpose: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na{sup 99m}TcO{sub 4} and laboratorial parameters in Wistar rats. Methods: twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day) during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na{sup 99m}TcO{sub 4} by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (% ATI/g) was determined. Biochemical dosages were performed. Results: there was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (p<0.05). Conclusion: The aqueous extract of Aloe vera facilitated the uptake of Na{sup 99m}TcO{sub 4} in organs of rats and it was responsible to a high increase of levels of AST and ALT. (author)

  7. Efficacy of Aloe Vera Supplementation on Prediabetes and Early Non-Treated Diabetic Patients: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

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    Yiyi Zhang

    2016-06-01

    Full Text Available The aim of this study was to evaluate evidence for the efficacy of aloe vera on managing prediabetes and early non-treated diabetes mellitus. We performed a systematic search of PubMed, Embase, and Cochrane Central Register of Controlled Trials until 28 January 2016. A total of five randomized controlled trials (RCTs involving 415 participants were included. Compared with the controls, aloe vera supplementation significantly reduced the concentrations of fasting blood glucose (FBG (p = 0.02; weighed mean difference [WMD]: −30.05 mg/dL; 95% confidence interval [CI]: −54.87 to −5.23 mg/dL, glycosylated hemoglobin A1c (HbA1c (p < 0.00001; WMD: −0.41%; 95% CI: −0.55% to −0.27%, triglyceride (p = 0.0001, total cholesterol (TC (p < 0.00001, and low density lipoprotein-cholesterol (LDL-C (p < 0.00001. Aloe vera was superior to placebo in increasing serum high density lipoprotein-cholesterol (HDL-C levels (p = 0.04. Only one adverse event was reported. The evidence from RCTs showed that aloe vera might effectively reduce the levels of FBG, HbA1c, triglyceride, TC and LDL-C, and increase the levels of HDL-C on prediabetes and early non-treated diabetic patients. Limited evidence exists about the safety of aloe vera. Given the small number and poor quality of RCTs included in the meta-analysis, these results are inconclusive. A large-scale, well-designed RCT is needed to further address this issue.

  8. PHYTOCHEMICAL SCREENING, TLC FINGERPRINTING AND ANTIMICROBIAL ACTIVITY OF METHANOLIC LEAF EXTRACT OF ALOE VERA AGAINST CLINICAL ISOLATES OF METHICILLIN RESISTANT Staphylococcus aureus

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    HARINATHA REDDY A

    2016-02-01

    Full Text Available Objective: The present study was carried out to investigate phytochemical properties, Thin layer chromatography (TLC fingerprinting and antimicrobial activity of methanolic leaf extract of Aloe vera. Methods: A. vera leaves were collected, dried and powdered. The leaf powder was subjected to methanolic extraction. Preliminary phyotochemical screening done by using standard procedures. TLC studies of the methanol leaf extract of A. vera were carried out by using two different solvent systems. Solvent system I consist of Chloroform: Methanol (12:2 and solvent system II consist of Ethyl acetate: Toluene: Formic acid (2.2:1.1:1.1. The antibacterial activity of the methanolic leaf extract of A. vera was studied using disc diffusion method against Staphylococcus aureus ATCC 2592 and Methicillin Resistant Staphylococcus aureus (MRSA.Result: Preliminary phytochemical analysis of methanolic leaf extract of A. vera revealed that presence of carbohydrates, glycosides, amino acids, phenolic compounds, steroids, terpenoids, tannins, saponins, flavonoids and alkaloids. The Rf values of methanolic leaf extract in solvent system I is 0.66 and solvent system II is 0.42. The methanolic leaf extract of A. vera exhibited antimicrobial activity on S. aureus ATCC 25923 and MRSA. Maximum zone of inhibition was observed at 50 mg/ml of methanolic leaf extract of A. vera.Conclusion: The present results suggest that methanolic leaf extract of A. vera have significant antibacterial activity against Staphylococcus aureus ATCC 2592 and Methicillin Resistant Staphylococcus aureus (MRSA.

  9. Ethanolic extract of Aloe vera ameliorates sciatic nerve ligation induced neuropathic pain

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    Swetha Kanyadhara

    2014-01-01

    Conclusion: The results of the present study validate the use of EEAV to treat neuropathic pain. This effect may be attributed to the decreased migration of neutrophils and due to the anti-oxidant properties of A. vera. Further studies to confirm the mechanism of action will help develop suitable A. vera formulations for neuropathic pain therapy .

  10. Cavity disinfection in minimally invasive dentistry - comparative evaluation of Aloe vera and propolis: A randomized clinical trial

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    A R Prabhakar

    2015-01-01

    Full Text Available Context: The survival of atraumatic restorative treatment (ART restorations would probably increase if near total elimination of cariogenic microorganisms could be done in the process of cavity cleaning before going ahead with the restoration. Thus, use of naturally occurring disinfecting agents for achieving this goal could herald a new beginning in the field of contemporary minimum intervention dentistry. Aims: To evaluate the efficacy of hand instruments in excavating dental caries and comparatively evaluate the roles of Aloe vera and propolis as potential cavity disinfecting agents after minimally invasive hand excavation of dental caries. Settings and Designs: Experimental, in vivo intergroup split mouth, randomized clinical trial. Subjects and Methods: The study included Group I (Control, Group II (A. vera and Group III (propolis. Ten patients with three teeth each have occlusal/occlusoproximal lesions suitable for ART were selected. Dentinal samples were collected three times from each tooth viz., preexcavation, postexcavation and postdisinfection of the cavities. These dentinal samples were subjected to microbiological analyses for total viable count. Statistical Analysis Used: Repeated measures of analysis of variance (ANOVA with Bonferroni post-hoc test and one-way ANOVA with Tukey post-hoc test. Results: In all the three groups, significant amount of bacteria were left behind after hand excavation. Group II and Group III, in which cavities were treated with A. vera and propolis extracts respectively, showed a significant reduction in the bacterial counts when compared to control the group. Conclusions: Hand excavation alone does not completely eliminate bacteria, which may predispose treated teeth to secondary caries. Both propolis and A. vera extracts can be used as potential natural disinfecting agents, thereby embracing the concept of phytotherapy in minimum intervention dentistry.

  11. Cavity disinfection in minimally invasive dentistry - comparative evaluation of Aloe vera and propolis: A randomized clinical trial

    Science.gov (United States)

    Prabhakar, A. R.; Karuna, Y. M.; Yavagal, C.; Deepak, B. M.

    2015-01-01

    Context: The survival of atraumatic restorative treatment (ART) restorations would probably increase if near total elimination of cariogenic microorganisms could be done in the process of cavity cleaning before going ahead with the restoration. Thus, use of naturally occurring disinfecting agents for achieving this goal could herald a new beginning in the field of contemporary minimum intervention dentistry. Aims: To evaluate the efficacy of hand instruments in excavating dental caries and comparatively evaluate the roles of Aloe vera and propolis as potential cavity disinfecting agents after minimally invasive hand excavation of dental caries. Settings and Designs: Experimental, in vivo intergroup split mouth, randomized clinical trial. Subjects and Methods: The study included Group I (Control), Group II (A. vera) and Group III (propolis). Ten patients with three teeth each have occlusal/occlusoproximal lesions suitable for ART were selected. Dentinal samples were collected three times from each tooth viz., preexcavation, postexcavation and postdisinfection of the cavities. These dentinal samples were subjected to microbiological analyses for total viable count. Statistical Analysis Used: Repeated measures of analysis of variance (ANOVA) with Bonferroni post-hoc test and one-way ANOVA with Tukey post-hoc test. Results: In all the three groups, significant amount of bacteria were left behind after hand excavation. Group II and Group III, in which cavities were treated with A. vera and propolis extracts respectively, showed a significant reduction in the bacterial counts when compared to control the group. Conclusions: Hand excavation alone does not completely eliminate bacteria, which may predispose treated teeth to secondary caries. Both propolis and A. vera extracts can be used as potential natural disinfecting agents, thereby embracing the concept of phytotherapy in minimum intervention dentistry. PMID:25821369

  12. Responses of broilers to Aloe vera bioactives as feed additive: The effect of different forms and levels of bioactives on performances of broilers

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    A.P Sinurat

    2002-06-01

    Full Text Available Feed additives are commonly used in poultry feed as growth promotors or to improve feed efficiency. Previous results showed that Aloe vera bioactives could improve feed efficiency in broilers. Therefore, a further study was designed in order to obtain optimum doses and application methods of bioactives for broiler chickens. Aloe vera was prepared in different forms (fresh gel, dry gel, fresh whole leaf or dry whole leaf. The aloe was supplemented into the feed with concentrations of 0.25; 0.5 and 1 g/kg (equal to dry gel. Standard diets with or without antibiotics were also included as control. The diets were fed to broilers from day old to 5 weeks and the performances were observed. Results showed that the aloe-bioactives did not significantly (P>0.05 affect final body weight of broilers as compared with the control. Supplementation of 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel significantly improved feed convertion by 4.7; 4.8 and 8.2%, respectively as compared with the control. This improvement was a result of reduction in feed intake or dry matter intake without reducing the weight gain. However, supplementation of whole aloe leafs could not improve feed convertion in boilers. It is concluded that the bioactives of Aloe vera could be used as feed supplement to improve feed efficiency in broilers with no deleterious effect on weight gain, carcass yield, abdominal fat levels and internal organs. The effective concentrations of aloe gell as a feed supplement based on dry matter convertion were from 0.25 g/kg fresh gel, 0.25 and 1.0 g/kg dry gel.

  13. Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera) on dentin stabilization using shear bond testing

    OpenAIRE

    Dakshita Joy Sinha; Natasha Jaiswal; Agrima Vasudeva; Paridhi Garg; Shashi Prabha Tyagi; Priyanka Chandra

    2016-01-01

    Introduction: The main objective of adhesive dentistry is to create an effective, durable union between the tooth structure and restorative material. However, degradation of adhesive dentine interface remains largely responsible for the relatively short lifetime of tooth colored resin restoration. Aim: The aim of the study is to compare the dentin collagen stabilization property of Chlorhexidine (CHX) and Aloe barbadensis Miller using shear bond strength testing. Materials and Methods: ...

  14. Evaluation and comparison of commercially available Aloe vera L. products using size exclusion chromatography with refractive index and multi-angle laser light scattering detection.

    Science.gov (United States)

    Turner, Carlton E; Williamson, David A; Stroud, Paul A; Talley, Doug J

    2004-12-20

    Raw materials supplied as Aloe vera L. (sometimes referred to as Aloe barbadensis) samples often contain different composition of low and high molecular weight components when analyzed by size exclusion chromatography. One major reason for variable compositions of commercial A. vera L. materials is that they are produced by different manufacturing techniques. Consistent composition of matter based upon a given standard has been difficult to define. In addition, the method of quantifying and characterization of these commercially available materials has not been agreed upon within the industry. The end user, whether a researcher, a manufacturer, a marketing arm of industry or the consumer, should know that they are receiving a consistent product. A blind study of 32 various A. vera L. samples from different manufacturers, and a prepared sample of fresh A. vera L. gel with the commercial, biologic drug Acemannan Immunostimulanttrade mark, were analyzed for content of high molecular weight (polysaccharides) material by size exclusion chromatography with refractive index detection (SEC/RI) and SEC/RI coupled with multi-angle laser light scattering (MALLS) detection. Results from the SEC/RI analysis showed significant variation in the high molecular weight content, and the MALLS analysis also showed significant variation versus SEC/RI. In addition, HPLC analysis of the anthraquinone content showed that all samples contained significantly less than that of the raw, unwashed aloe gel. The variation of results from all analysis is attributed to differing methods in which the samples were processed by the different manufacturers.

  15. Effects of plant sterols derived from Aloe vera gel on human dermal fibroblasts in vitro and on skin condition in Japanese women

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    Tanaka M

    2015-02-01

    Full Text Available Miyuki Tanaka,1 Eriko Misawa,1 Koji Yamauchi,1 Fumiaki Abe,1 Chiaki Ishizaki2 1Functional Food Research Department, Food Science and Technology Institute, Morinaga Milk Industry Co, Ltd, Zama, Kanagawa, 2Ebisu Skin Research Center, Inforward, Inc., Tokyo, Japan Background: Aloe is known for its topical use for treating wounds and burns. Many previous studies reported the healing effects of Aloe vera. However, there are few clinical studies on the effect of orally administered A. vera gel on the skin. Aloe sterols are a type of plant sterols that have the capability to regulate the metabolism of glucose and lipids. In a recent study, we confirmed that ingested Aloe sterols reached the peripheral tissues through the bloodstream. However, their influence on dermal fibroblasts has not been investigated. Methods: First, we investigated the capability of Aloe sterols (cycloartenol and lophenol to stimulate human dermal fibroblasts in vitro. Then, we investigated the effect of intake of Aloe vera gel powder (AVGP containing 40 µg Aloe sterols on the skin conditions in Japanese women with dry skin in a randomized, double-blind, placebo-controlled trial. Results: After cocultivation with Aloe sterols, the production of collagen and hyaluronic acid increased by approximately two-fold and 1.5-fold, and gene expression levels of these enzymes responsible for their synthesis were also observed in human dermal fibroblasts. An increase in arm skin hydration was observed at 8 weeks in the AVGP group, whereas a slight decrease in arm skin hydration was noted in the placebo group. However, there was no statistical difference between AVGP and placebo groups in skin moisture. In subgroup analysis, the change in the mean wrinkle depth was significantly lower in the AVGP group than in the control group. In addition, percent body fat after 8 weeks was significantly lower in the AVGP group. No AVGP intake-dependent harmful phenomenon was observed during the intake

  16. Oral Administration Of Aloe Vera (l.) Burm. F. (xanthorrhoeaceae) And Honey Improves The Host Body Composition And Modulates Proteolysis Through Reduction Of Tumor Progression And Oxidative Stress In Rats

    OpenAIRE

    Tomasin, Rebeka; de Andrade, Rafael Siqueira; Gomes-Marcondes, Maria Cristina Cintra

    2016-01-01

    Oxidative stress has a dual role in cancer; it is linked with tumorigenic events and host wasting, as well as senescence and apoptosis. Researchers have demonstrated the importance of coadjuvant therapies in cancer treatment, and Aloe vera and honey have immunomodulatory, anticancer, and antioxidant properties. The preventive and therapeutic effects of Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) and honey in tumor progression and host wasting were analyzed in Walker 256 carcinoma-bearing rats....

  17. The influence of Aloe vera and xenograft XCB toward of bone morpho protein 2 BMP2 expression and amount of osteoblast of alveolar bone induced into tooth extraction sockets Cavia cobaya

    Directory of Open Access Journals (Sweden)

    Utari Kresnoadi

    2014-12-01

    Full Text Available Tooth extraction can cause inflammation leading to alveolar ridge resorption. In addition, prominent ridge has crucial role for making denture su-ccessfully. Thus, socket preservation is needed to prevent greater alveolar ridge resorption. An innovative material, a combination of Aloe vera and xe-nograft (XCB, is then considered as a biogenic stimulator that can reduce inflammation, as a result, the growth of alveolar bone is expected to be impro-ved. This research is aimed to prove whether the mixture of Aloe vera and xenograft can stimulate BMP2 and increase osteoblasts. Forty-eight Cavia co-baya animals were divided into eight groups each of which consisted of six animals. The mandibular incisors of those Cavia cobaya animals were then extracted and filled with PEG as Group Control, XCB as Group XCB, Aloe vera as Group Aloe vera, and a combination of Aloe vera +XCB as Group Aloe vera +XCB. Next, the first four groups were sacrificed seven days after extraction, and the second four groups were sacrificed 30 days after extrac-tion. And then, immunohistochemical and histopathology examinations were conducted to examine BMP2 expression and osteoblasts. Based on the re-sult known that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblasts. It can be concluded that the mixture of Aloe vera and xenograft can increase BMP2 expression and amount of osteoblast cel . It can be used as an alternative material to increase the growth of alveolar bone after extraction.

  18. Effects of Aloe vera cream on chronic anal fissure pain, wound healing and hemorrhaging upon defection: a prospective double blind clinical trial.

    Science.gov (United States)

    Rahmani, N; Khademloo, M; Vosoughi, K; Assadpour, S

    2014-01-01

    Aloe vera is a medicinal plant that promotes wound healing in burn injuries. A prospective clinical trial was conducted to evaluate the effects of a topical cream containing 0.5% Aloe vera juice powder in the treatment of chronic anal fissures. The aloe cream was applied by the patients to the wound site 3 times per day for 6 weeks following the instructions of a physician. Pain was assessed with a visual analog scale before treatment and at the end of each week of treatment. Wound healing and the amount and severity of bleeding were examined and evaluated before and at the end of each week of treatment. There were statistically significant differences in chronic anal fissure pain, hemorrhaging upon defection and wound healing before and at the end of the first week of treatment also in comparison with control group (p aloe vera juice was an effective treatment for chronic anal fissures. This is a promising result indicating that further comparative studies are justified.

  19. An in vivo and in vitro investigation of the effect of Aloe vera gel ethanolic extract using animal model with diabetic foot ulcer

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    Mohan Daburkar

    2014-01-01

    Full Text Available Aim: To examine the preventive effect of Aloe vera gel ethanolic extract using diabetic foot ulcer (DFUs protocol in Wistar rats. Materials and Methods: Male Wistar rats were divided into untreated control (Group I, untreated DFUs (Group II, DFUs treated with A. vera gel ethanolic extract (Group III, DFUs treated with topical A. vera gel (Group IV, DFUs treated with A. vera gel ethanolic extract and topical A. vera gel (Group V. The rats in the treatment groups were daily administered the A. vera gel and ethanolic extract for 9 days. Fasting blood glucose levels and percentage of wound ulcer contraction were measured on day 3, 6, and 9. Statistical Analysis used: The results are expressed as a mean ± Standard Error Mean (SEM. Data were analyzed using one-way analysis of variance (ANOVA after Newman-Keuls test. P < 0.05 were considered statistically significant in all cases. Results: Oral administration of A. vera gel ethanolic extract at a dose of 300 mg/kg body weight per day to diabetic rats for a period of 9 days resulted in a significant reduction in fasting blood glucose and a significant improvement in plasma insulin. Topical application of A. vera gel at a dose 30 mg/kg body weight per day to streptozotocin (STZ-induced diabetic rats for a period of 9 days resulted in no change in blood glucose and plasma insulin. Oral administration as well as topical application of A. vera gel ethanolic extract and gel significantly reduced the blood glucose, improved the plasma insulin, and significantly increased DNA and glycosaminoglycans (GAGs to improve the wound ulcer healing as well as the breaking strength on day 9. Conclusions: Present findings provide a scientific rationale for the use of A. vera gel ethanolic extract, and showed that the gel attenuated the diabetic foot wound in rats.

  20. Determinación del efecto del gel de Aloe vera, sobre la glicemia en caninos sanos

    OpenAIRE

    María Mercedes Tamayo; Anastasia Cruz-Carrillo

    2011-01-01

    El papel desempeñado por la glucosa en los organismos es  fundamental, ya que es  la forma más importante por la cual los carbohidratos son absorbidos desde el tracto intestinal, con diversos factores que  influyen en los niveles de glucosa del  organismo.  La  causa más  frecuente  de hiperglucemia  en  los  perros  es  el  daño pancreático, hiperadrenocorticismo  e hipersomatotropismo. Así, al evaluar el efecto del gel de Aloe vera sobre  la glicemia en caninos,tras su administración oral, ...

  1. Do Aloe vera and Ageratum conyzoides enhance the anti-microbial activity of traditional medicinal soft soaps (Osedudu)?

    Science.gov (United States)

    Moody, J O; Adebiyi, O A; Adeniyi, B A

    2004-05-01

    The Nigerian traditional soft soaps prepared using varied locally sourced raw materials such as cocoa pod ash (Theobroma cacao) palm kernel shaft ash (Elaies guineensis) have been evaluated for their physico-chemical properties and anti-microbial activities using standard pharmacopoeia protocols and an in-vitro agar diffusion bioassay method. The anti-microbial evaluation was done with and without incorporation of Aloe vera and Ageratum conyzoides extractives into the soap samples. Results showed that the physico-chemical properties of the soaps are dependent on the raw materials utilised. The incorporated medicinal plants used in this study, however, did not show any significant effect on the anti-microbial activities exhibited by the various soaps against the bacterial and fungal test organisms.

  2. Aloe vera plant-extracted solution hydrothermal synthesis and magnetic properties of magnetite (Fe3O4) nanoparticles

    Science.gov (United States)

    Phumying, Santi; Labuayai, Sarawuth; Thomas, Chunpen; Amornkitbamrung, Vittaya; Swatsitang, Ekaphan; Maensiri, Santi

    2013-06-01

    Magnetite (Fe3O4) nanoparticles have been successfully synthesized by a novel hydrothermal method using ferric acetylacetonate (Fe(C5H8O2)3) and aloe vera plant-extracted solution. The influences of different reaction temperatures and times on the structure and magnetic properties of the synthesized Fe3O4 nanoparticles were investigated. The synthesized nanoparticles are crystalline and have particle sizes of ˜6-30 nm, as revealed by transmission electron microscopy (TEM). The results of X-ray diffraction (XRD), High resolution TEM (HRTEM) and selected area electron diffraction (SAED) indicate that the synthesized Fe3O4 nanoparticles have the inverse cubic spinel structure without the presence of any other phase impurities. The hysteresis loops of the Fe3O4 nanoparticles at room temperature show superparamagnetic behavior and the saturation magnetization of the Fe3O4 samples increases with increasing reaction temperature and time.

  3. EFFECT OF DIETARY SUPPLEMENTATION OF Melissa Officinalis and Aloe Vera ON HEMATOLOGICAL TRAITS, LIPID OXIDATION OF CARCASS AND PERFORMANCE IN RAINBOW TROUT (Oncorhynchus Mykiss

    Directory of Open Access Journals (Sweden)

    A. FARAHI

    2012-02-01

    Full Text Available This was conducted investigate the effect of feeding lemon balm (Melissa officinalis and Aloe (Aloe vera on growth performance, hematological parameters and oxidative stability of rainbow trout. 360 uniform rainbow trout (20.87±0.25 g were divided into 3 groups, and fed standard diets supplemented with ground lemon balm (2%, L group or supplemented with Aloe (1%, A group and without supplementation (Control, C group. Growth performance and body composition were not influenced by plant supplementation. Survival rate of fish was promoted in diets supplemented with herbs, significantly (P0.05 were not observed in RBC and Hb in treatments (P>0.05. Results of thiobarbituric acid value (TBA showed that lemon balm and Aloe herbs could be protective against lipid peroxidation in fish meat during chilling storage (4°C, 7 days.

  4. Structural characterization and solution properties of a galacturonate polysaccharide derived from Aloe vera capable of in situ gelation.

    Science.gov (United States)

    McConaughy, Shawn D; Stroud, Paul A; Boudreaux, Brent; Hester, Roger D; McCormick, Charles L

    2008-02-01

    A series of highly purified galacturonate polysaccharides have been extracted from the Aloe vera plant and analyzed in terms of chemical composition and molecular weight. This Aloe vera polysaccharide (AvP) has been found to exist as a high molecular weight species and possess a unique chemical composition, including a high galacturonic acid (GalA) content and low degree of methyl ester substitution. These factors facilitate gel formation upon exposure to low concentrations of calcium ions, leading to potential application in formulations designed for in situ nasal or subcutaneous protein delivery. Thorough examination of classic dilute solution properties, the [eta]-M(w), and R(g)-M(w) relationships, persistence length (L(p)), and inherent chain stiffness (B parameter), indicate an expanded random coil in aqueous salt solutions. The critical concentration for transition from dilute to concentrated solution, C(e), was determined by measuring both the zero shear viscosity (eta(o)) and fluorescence emission of the probe molecule 1,8-anilino-1-naphthalene sulfonic acid (1,8-ANS) as a function of polymer concentration. Examination of zeta potential and C(e) as a function of ionic strength indicates that the shift in C(e) from 0.60 to 0.30 wt % is related to an increased occurrence of intermolecular interactions at high salt concentrations. Additionally, dynamic rheology data are presented highlighting the ability of AvP to form gels at low polymer and calcium ion concentrations, exemplifying the technological potential of this polysaccharide for in situ drug delivery.

  5. Review of the efficacy of green tea, isoflavones and aloe vera supplements based on randomised controlled trials.

    Science.gov (United States)

    Williamson, Gary; Coppens, Patrick; Serra-Majem, Lluís; Dew, Tristan

    2011-12-01

    We assess the evidence for health benefits of three commonly consumed plant food supplements (PFS), green tea, isoflavone and aloe vera, based on published systematic reviews of randomised controlled trials (RCTs). Whilst the potential benefits of green tea have been reported in a wide range of health areas, it is only in the area of the metabolic syndrome that the number of RCTs is approaching sufficient to judge such efficacy. Isoflavone supplements are widely used, and RCTs indicate that they affect bone resorption at lower doses in postmenopausal women undergoing estrogen-related bone loss, but this is only translated to attenuation of bone loss at higher doses of isoflavones. A systematic review on RCTs concluded that the effects of isoflavones on hot flashes in postmenopausal women were highly variable and no conclusions could be drawn. Despite the popularity of aloe vera as a PFS, the evaluation of its efficacy as a coadjuvant therapy for certain metabolic or digestive pathologies remains scarce; it constitutes a typical example of a naturally occurring ingredient whose efficacy in topical applications presupposes its efficacy in systemic applications. Nevertheless, its possible toxic effects on oral consumption call for caution in its utility as a PFS. Since 2007, efficacy evaluation of PFS in Europe has been covered by European Union Nutrition and Health Claims legislation. The European Food Safety Authority has adopted an approach relying on RCTs, while medicinal effects are accepted based on traditional use. In general, there are insufficient RCTs for claims to be made, and conclusive results on PFS should be obtained in the future by conducting studies with more homogeneous populations, by using supplements with optimised and measured bioavailability, and by conducting larger RCTs.

  6. Effect of Aloe vera extract on the improvement of the respiratory activity of leukocytes of matrinxã during the transport stress

    Directory of Open Access Journals (Sweden)

    Fábio Sabbadin Zanuzzo

    2012-10-01

    Full Text Available This study evaluated the effect of extract of Aloe vera in the transport water of matrinxã (Brycon amazonicus fish on stress response and leukocyte respiratory activity. Fish was transported for 4 h in water containing Aloe at levels 0; 0.02; 0.2 and 2 mg/L, and sampled before transport 2, 4, 24 and 96 h after for determination of plasma glucose and respiratory activity of leukocytes. An additional in vitro assay was conducted with another fish species, pacu (Piaractus mesopotamicus, to test the respiratory burst of leukocytes exposed to Aloe extract (0.0, phosphate-buffered saline (PBS only at 0.1, 0.2, 0.5 and 1 mg/L. Plasma glucose increased after 2 and 4 h of transport and returned to control levels within 24 h, but the addition of Aloe in the transport water did not affect the level of blood glucose. However, at 2 h of transport, Aloe enhanced the respiratory activity of leukocytes in a dose-dependent way. The highest value of respiratory burst activity of leukocytes was observed in the fish transported in water containing Aloe at 2 mg/L. The enhancing effect of the plant extract on the production of oxygen radicals was confirmed in vitro in leukocytes of pacu incubated in Aloe at concentrations 0.1 and 0.2 mg/L. The results suggest that Aloe vera is a modulator of the immune system in fish improving the innate immune response tested.

  7. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge.

    Science.gov (United States)

    Gabriel, Ndakalimwe Naftal; Qiang, Jun; Ma, Xin Yu; He, Jie; Xu, Pao; Liu, Kai

    2015-10-01

    The current study investigated the effects of dietary Aloe vera on plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities of GIFT-tilapia juveniles under Streptococcus iniae challenge. Five dietary groups were designed including a control and 100 % Aloe powder incorporated into a tilapia feed at 0.5, 1, 2, and 4 %/kg feed, which were administered for 8 weeks. Fish fed dietary Aloe at 4 %/kg feed significantly reduced in total cholesterol, while triacylglycerol reduced (P Aloe/kg feed compared to unsupplemented ones. High-density lipoprotein was significantly elevated in fish fed 0.5 and 1 % Aloe/kg feed compared to unsupplemented ones, and no significant changes (P > 0.05) were noted in low-density lipoprotein among test groups. Furthermore, high activities of superoxide dismutase, catalase, and glutathione peroxide in liver tissues were observed in Aloe-supplemented fish compared to unsupplemented ones, before and after S. iniae challenge (7.7 × 10(6) CFU cells/mL). Variations were also noted in malondialdehyde activity throughout the trial, but no significant difference (P > 0.05) was observed between groups. Meanwhile, Aloe-supplemented fish reduced serum aspartate and alanine aminotransferase (AST and ALT) activities before and after challenge. Based on the second-order polynomial regression analysis, dietary Aloe inclusion levels less than or equal to 1.88, 1.86, and 2.79 %/kg feed were determined to be suitable in improving plasma lipid profile status, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia in this study, respectively. Thus, A. vera extracts may be recommended as a tilapia feed supplement to enhance fish antioxidant and hepatoprotective capacities, especially during disease outbreaks.

  8. Examination of an aloe vera galacturonate polysaccharide capable of in situ gelation for the controlled release of protein therapeutics

    Science.gov (United States)

    McConaughy, Shawn David

    A therapeutic delivery platform has been investigated with the ultimate goal of designing a sustained protein release matrix utilizing an in-situ gelling, acidic polysaccharide derived from the Aloe vera plant. The Aloe vera polysaccharide (AvP) has been examined in order to determine how chemical composition, structure, molecular weight and solution behavior affect gelation and protein/peptide delivery. Correlations are drawn between structural characteristics and solution behavior in order to determine the impact of polymer conformation and solvation on gel formation under conditions designed to simulate nasal applications. Steady state and dynamic rheology, classic and dynamic light scattering, zeta potential, pulse field gradient nuclear magnetic resonance and fluorescence spectroscopy have been employed to gain insight into the effects of galacturonic acid content, degree of methylation, entanglement and ionic strength on both solution behavior and the hydrogel state which ultimately governs protein/peptide release. This dissertation is divided into two sections. In the first section, a series of Aloe vera polysaccharides (AvP), from the pectin family have been structurally characterized indicating high galacturonic acid (GalA) content, low degree of methylester substitution (DM), low numbers of rhamnose residues and high molecular weight with respect to pectins extracted from traditional sources. The behavior of AvP was examined utilizing dilute solution, low-shear rheological techniques for specific molecular weight samples at selected conditions of ionic strength. From these dilute aqueous solution studies, the Mark-Houwink-Sakurada (MHS) constants (K and alpha), persistence length (Lp) and inherent chain stiffness (B parameter) were determined, indicating an expanded random coil in aqueous salt solutions. The critical concentration for transition from dilute to concentrated solution, C e, was determined by measuring both the zero shear viscosity and

  9. Development of bilayer floating tablet of amoxicillin and Aloe vera gel powder for treatment of gastric ulcers.

    Science.gov (United States)

    Ranade, Arati N; Wankhede, Sonali S; Ranpise, Nisharani S; Mundada, Mayur S

    2012-12-01

    Usual treatment for Helicobacter pylori-induced peptic ulcer includes a 'triple therapy' consisting of two antibiotics (amoxicillin and clarithromycin) and a proton pump inhibitor (omeprazole). The objective of this project work was defined with a view to retain the drug in stomach for better antiulcer activity and substituting one of the synthetic drugs in this therapy with a herbal alternative. Hence, aim of the present work was to design and develop a bilayer floating tablet of amoxicillin and Aloe vera gel powder for the treatment of peptic ulcer. A. vera gel powder is used for its cytoprotective action. Bilayer floating tablets were prepared by applying direct compression technique. The proportion of sodium bicarbonate and citric acid was adjusted to get the least possible lag time with good matrix integrity and total floating time. Polymer concentration was adjusted to get the maximum release in 8 h. The formulation was developed using hydroxypropyl methyl cellulose (HPMC) K4M and HPMC K100M in a ratio of 85:15 along with 1:4 ratio of effervescent agents was found to give floating lag time of less than 1 min with total floating time of more than 8 h and 97.0% drug release in 8 h. In vivo study in rats meets the requirement of antiulcer activity for bilayer tablet in comparison to single amoxicillin as standard.

  10. EVALUATION OF FREEZE DRIED ALOE VERA AND NOPAL CACTUS FOR POSSIBLE HEALTH TREATMENTS BY COMPARISON OF ANTIOXIDANT PROPERTIES AND FREE RADICAL INHIBITION.

    Science.gov (United States)

    Rodriguez, Kenneth R; Jones, Anthony E; Belmont, Barbara

    2014-01-01

    The goal of this project was to characterize the antioxidant powers of lyophilized Aloe Vera (Aloe barbadensis) and Nopal Cactus (Opuntia ficus-indica) by quantifying the phenolics content and radical scavenging abilities of preparations derived from these plants. Extracts of these lyophylized succulents were assayed for phenolic compounds by the Folin Ciocalteau method and compared for free radical scavenging capability by the DPPH method. We found that even though the Aloe lyophilizate extract contained more phenolic content, the Nopal lyophilizate exhibited better free radical scavenging ability. Aloe Vera extract contained 0.278 g/L of phenolic content and exhibited 11.1% free radical inhibition, with a free radical scavenging rate constant of 0.177±0.015 min(-1). Nopal Cactus extract contained 0.174 g/L of phenolic content and exhibited 13.2% free radical inhibition, with a free radical scavenging rate constant of 0.155±0.009 min(-1). These results showed Nopal to have greater antioxidant potency than Aloe.

  11. THE CHEMICAL CONSTITUENTS OF ALOE VERA L.%库拉索芦荟化学成分的研究

    Institute of Scientific and Technical Information of China (English)

    肖志艳; 陈迪华; 斯建勇; 涂光忠; 马立斌

    2000-01-01

    目的:从中药库拉索芦荟(Aloe vera L.)中寻找新的降糖及抗炎活性成分.方法:利用多种柱色谱方法进行分离纯化,根据光谱数据和化学方法进行结构鉴定.结果:从库拉索芦荟乙醇浸膏的乙酸乙酯部分分离鉴定了11个化合物,分别为:芦荟色苷G(aloeresin G,1),异芦荟色苷D(isoaloeresin D,2),芦荟大黄素(aloe-emodin,3),芦荟苷A(babarloin A, 4),8-O-甲基-7-羟基芦荟苷B(8-O-methyl-7-hydroxyaloin B,5),elgonica-dimer A(6),elgonica-dimer B(7),feralolide(8),何伯烷-3-醇(hopan-3-ol,9),β-谷甾醇(β-sitosterol,10)及胡萝卜苷(daucosterol,11).结论:化合物1为新化合物,命名为芦荟色苷G.

  12. Humoral responses of broiler chickens challenged with NDV following supplemental treatment with extracts of Aloe vera, Alma millsoni, Ganoderma lucidum and Archachatina marginata

    Science.gov (United States)

    Eghafona, Nosahkare'Odeh

    2015-01-01

    Aim of the study The significance of nutritional supplements for immunity has been documented. Locally sourced extracts used in alternative medicine were studied to determine their potential effects on antibody production and humoral responses in viral challenged birds. Method Three hundred and eighty birds were distributed into 19 groups of 20 birds each. Following acclimatization for 16 days, they were fed with standard broilers feed and water ad libitum. Group A was supplemented with Aloe vera (AV) extract, group B was given Alma millsoni (AM) extract, group C was given Archachatina marginata (AMS) extract and group D was given Ganoderma lucidum (GL) extract, and group E was the control group. Extract concentrations of 50 mg, 100 mg and 150 mg were given to three subsets of each treatment group for 30 days. Birds were then challenged with intramuscular administration of 0.2 ml of 50% Embryo Lethal Dose of saline suspension of the challenge strain of Newcastle Disease Virus (NDV) on the 30th day, and were examined for clinical signs and symptoms. Serum from venous blood was used for antibody and immunological assay. Results Aloe vera at 50 µg and A. millsoni extracts supplementations yielded a significant antibody titre (p extract of Ganoderma and A. marginata, pretreatment with A. millsoni extract and a lower dosage of Aloe vera enhanced the ability to mount humoral responses against viral infection in broiler chickens. PMID:26648773

  13. A randomized clinical study for comparative evaluation of Aloe Vera and 0.2% chlorhexidine gluconate mouthwash efficacy on de-novo plaque formation

    Science.gov (United States)

    Chhina, Shivjot; Singh, Avnish; Menon, Ipseeta; Singh, Rickypal; Sharma, Anubhav; Aggarwal, Vartika

    2016-01-01

    Objective: To comparatively assess the antiplaque efficacy of Aloe vera mouthwash and 0.2% chlorhexidine gluconate mouthwash on de novo plaque formation. Materials and Methods: This was a randomized, single blind, parallel, controlled clinical study with 90 healthy participants, with mean age of 27.19 ± 12.08 years. After thorough oral prophylaxis, participants were instructed to discontinue mechanical plaque control. Participants were divided randomly into three groups; pure Aloe vera mouthwash was dispensed to the test group; control group received 0.2% chlorhexidine gluconate mouthwash; in Placebo group, flavored distilled water was used as oral rinse twice daily. Effect on 4-day de novo plaque formation was assessed by comparing pre-rinsing Quigley Hein Modified Plaque Scores were analyzed statistically using analysis of variance and Student's t-test. Results: Post-rinsing control group showed the least plaque score which was comparable to the test group. Both the control group and test group showed significant difference with the placebo group. Conclusions: Herbal mouthwash containing Aloe vera mouthwash has comparable antiplaque efficacy as the gold standard 0.2% chlorhexidine gluconate with fewer side effects and can be considered as an alternative. PMID:27382543

  14. 芦荟对静脉炎的疗效观察%Aloe vera to curative effect observation of phlebitis

    Institute of Scientific and Technical Information of China (English)

    袁君兰

    2013-01-01

    目的探讨芦荟对静脉炎的疗效。方法将48例出现化学性、机械性静脉炎的患者分为观察组和对照组。观察组从出现静脉炎开始用鲜芦荟汁外涂沿血管走向敷,4~6次/d,7天为1个疗程,直到静脉炎消失;对照组取适量的50%硫酸镁溶液用纱布湿敷于静脉炎处皮肤,每日2~3次,7天为1个疗程,直到静脉炎消失。两组持续治疗7天后比较疗效。结果芦荟治疗静脉炎疗效明显优于50%硫酸镁湿敷组(p<0.01)。结论采用鲜芦荟汁外涂外敷对于治疗静脉炎效果显著且安全无不良反应。%objective to explore the curative effect of aloe vera to phlebitis. Methods: 48 cases with chemical, mechanical phlebitis patients divided into the observation group and the control group. The observation group from appear phlebitis start with fresh aloe juice besmear outside along the blood vessels to apply, 4~6 times/d, 7 days for a period of treatment, until phlebitis disappear; Control group apply adequate amount of 50% magnesium sulfate solution with wet gauze mask to the phlebitis place skin, 2~3 times daily for 7 days for a period of treatment, until phlebitis disappear. Two groups of continuous treatment of 7 days effect comparison. Results aloe treat phlebitis curative effect was better than 50% magnesium sulfate wet apply group (p < 0.01). Conclusion using fresh aloe juice external coating for the treatment of external phlebitis effect is remarkable and safety and no adverse reaction.

  15. Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera) on dentin stabilization using shear bond testing

    Science.gov (United States)

    Sinha, Dakshita Joy; Jaiswal, Natasha; Vasudeva, Agrima; Garg, Paridhi; Tyagi, Shashi Prabha; Chandra, Priyanka

    2016-01-01

    Introduction: The main objective of adhesive dentistry is to create an effective, durable union between the tooth structure and restorative material. However, degradation of adhesive dentine interface remains largely responsible for the relatively short lifetime of tooth colored resin restoration. Aim: The aim of the study is to compare the dentin collagen stabilization property of Chlorhexidine (CHX) and Aloe barbadensis Miller using shear bond strength testing. Materials and Methods: Occlusal reduction was done in sixty extracted human mandibular molars to expose the mid coronal dentin and divided into three groups n = 20. Following the surface pretreatment (Group 1 = control, Group 2 = CHX, Group 3 = Aloevera), dentine bonding agent and composite resin were applied and cured. The specimens were then subjected to shear bond strength testing. Results: From the results analyzed, it was noted that there was statistically significant difference between the groups Control and CHX and Control and A. barbadensis Miller (P 0.05). Hence, the following result for the shear bond strengths to dentin was obtained: Control < CHX ≈ A. barbadensis Miller. Conclusion: CHX and A. barbadensis Miller, as pretreatment agents of acid demineralized dentin collagen, has no adverse effect on the immediate shear bond strength of a two-step etch and rinse adhesive to dentin. PMID:27656056

  16. O uso do Aloe sp (aloe vera em feridas agudas e crônicas: revisão integrativa

    Directory of Open Access Journals (Sweden)

    Lucélia Terra Chini

    2017-01-01

    Full Text Available Objetivos: buscar evidencias disponibles en la literatura acerca del uso de Aloe sp (sábila en la cicatrización de heridas agudas y crónicas. Materiales y método: se trata de una revisión integradora realizada en las bases de datos LILACS, PubMed y Scopus, en el periodo de febrero a marzo del 2015. La búsqueda resultó en 178 publicaciones. Resultados: siete estudios constituyeron la revisión, los cuales involucraron personas con heridas de cesárea, heridas de episiotomía, quemaduras, área donadora de injerto, heridas posthemorroidectomía y heridas fisuarias anales crónicas. Se evidenció que la sábila promueve la cicatrización de heridas, además de disminuir el dolor en fisuras anales crónicas y quemaduras. Conclusiones: la sábila representa una nueva terapéutica en el tratamiento de heridas; sin embargo, las evidencias disponibles sobre su eficacia y seguridad son ineficientes para legitimar su uso en la cicatrización de heridas agudas y crónicas; por lo tanto, no se pueden generalizar.

  17. Protective effects of Aloe vera-based diets in Eimeria maxima-infected broiler chickens

    Science.gov (United States)

    Aloes have been widely used for a broad range of pharmacological activities, including parasitic problems. Avian coccidiosis is the most costly and wide-spread parasitic disease in the poultry industry, and has been mainly controlled by the use of chemotherapeutic agents. Due to the emergence of dru...

  18. Comparative study on the content of polysaccharide in Aloe barbadensis Miller and Aloe ;vera L. Var. Chinensis (Haw.) Berger%库拉索芦荟与斑纹芦荟的多糖含量比较研究

    Institute of Scientific and Technical Information of China (English)

    唐建红; 刘川玉; 何洁

    2016-01-01

    Objective To determine the content of polysaccharide in Aloe barbadensis Miller and Aloe vera L. Var. Chinensis(Haw.)Berger. Methods Carubinose was used as reference substance, measured and compared polysaccharide content with the method of UV spectrophotometry at 610 nm absorbance of growth years for 1, 2, 3, 4 and 5 years of Aloe barbadensis Miller and Aloe vera L. Var. Chinensis(Haw.)Berger. Results The mannose and the absorbance was linear (r=0.998 5) in the range of 0.05 - 0.60 g/L, and the content of Aloe barbadensis Miller polysaccharide in the measured growth period was respectively 1.201, 1.485, 1.665, 2.355 and 5.040 mg/g. The content of polysaccharide in Aloe vera L. Var. Chinensis(Haw.)Berger was 0.780, 0.900, 1.035, 1.650 and 2.355 mg/g. Conclusions The content of polysaccharide in Aloe barbadensis Miller and Aloe vera L. Var. Chinensis(Haw.)Berger differed in growth years, which increased with aging and polysaccharide content in the same age of Aloe barbadensis Miller was significantly higher than that of the Aloe vera L. Var. Chinensis(Haw.)Berger.%目的:测定不同年限的库拉索芦荟与斑纹芦荟的多糖含量。方法以D-甘露糖为对照品,采用紫外分光光度法,测定生长年限为1、2、3、4及5年的库拉索芦荟和斑纹芦荟的多糖含量,并对其进行比较分析。结果甘露糖在0.05~0.60 g/L范围内与吸光度呈良好的线性关系(r=0.9985),测得生长年限为1、2、3、4及5年的库拉索芦荟多糖含量分别为1.201、1.485、1.665、2.355、5.040 mg/g;斑纹芦荟的多糖含量分别为0.780、0.900、1.035、1.650、2.355 mg/g。结论不同生长年限的库拉索芦荟和斑纹芦荟的多糖含量有差别,其含量随年限增长而增加,相同年限库拉索芦荟的多糖含量明显高于斑纹芦荟。

  19. Effects of water stress and light intensity on chlorophyll fluorescence parameters and pigments of Aloe vera L.

    Science.gov (United States)

    Hazrati, Saeid; Tahmasebi-Sarvestani, Zeinolabedin; Modarres-Sanavy, Seyed Ali Mohammad; Mokhtassi-Bidgoli, Ali; Nicola, Silvana

    2016-09-01

    Aloe vera L. is one of the most important medicinal plants in the world. In order to determine the effects of light intensity and water deficit stress on chlorophyll (Chl) fluorescence and pigments of A. vera, a split-plot in time experiment was laid out in a randomized complete block design with four replications in a research greenhouse. The factorial combination of three light intensities (50, 75 and 100% of sunlight) and four irrigation regimes (irrigation after depleting 20, 40, 60 and 80% of soil water content) were considered as main factors. Sampling time was considered as sub factor. The first, second and third samplings were performed 90, 180 and 270 days after imposing the treatments, respectively. The results demonstrated that the highest light intensity and the severe water stress decreased maximum fluorescence (Fm), variable fluorescence (Fv)/Fm, quantum yield of PSII photochemistry (ФPSII), Chl and photochemical quenching (qP) but increased non-photochemical quenching (NPQ), minimum fluorescence (F0) and Anthocyanin (Anth). Additionally, the highest Fm, Fv/Fm, ФPSII and qP and the lowest NPQ and F0 were observed when 50% of sunlight was blocked and irrigation was done after 40% soil water depletion. Irradiance of full sunlight and water deficit stress let to the photoinhibition of photosynthesis, as indicated by a reduced quantum yield of PSII, ФPSII, and qP, as well as higher NPQ. Thus, chlorophyll florescence measurements provide valuable physiological data. Close to half of total solar radiation and irrigation after depleting 40% of soil water content were selected as the most efficient treatments.

  20. In vitro effect of Aloe vera, Coriandrum sativum and Ricinus communis fractions on Leishmania infantum and on murine monocytic cells.

    Science.gov (United States)

    Rondon, Fernanda C M; Bevilaqua, Claudia M L; Accioly, Marina P; Morais, Selene M; Andrade-Junior, Heitor F; Machado, Lyeghyna K A; Cardoso, Roselaine P A; Almeida, Camila A; Queiroz-Junior, Eudson M; Rodrigues, Ana Caroline M

    2011-06-10

    In South America, visceral leishmaniasis is a zoonosis caused by the protozoan species Leishmania infantum (syn. L. chagasi) and is primarily transmitted through the bite of the female Lutzomyia longipalpis. Its main reservoir in urban areas is the dog. The application of control measures recommended by health agencies have not achieved significant results in reducing the incidence of human cases, and the lack of effective drugs to treat dogs resulted in the prohibition of this course of action in Brazil. Therefore, it is necessary to search new alternatives for the treatment of canine and human visceral leishmaniasis. The objectives of this study were to evaluate the in vitro effect of fractions from Aloe vera (aloe), Coriandrum sativum (coriander), and Ricinus communis (castor) on promastigotes and amastigotes of L. infantum and to analyze the toxicity against the murine monocytic cells RAW 264.7. To determine the viability of these substances on 50% parasites (IC50), we used a tetrazolium dye (MTT) colorimetric assay (bromide 3-4.5-dimethylthiazol-2-yl-2,5-dephenyltetrazolium), and on amastigotes we performed an in situ ELISA. All fractions were effective against L. infantum promastigotes and did not differ from the positive control pentamidine (p>0.05). However, the R. communis ethyl acetate and chloroform fractions, as well as the C. sativum methanol fraction, were the most effective against amastigotes and did not differ from the positive control amphotericin B (p>0.05). The R. communis ethyl acetate fraction was the least toxic, presenting 83.5% viability of RAW 264.7 cells, which was similar to the results obtained with amphotericin B (p>0.05). Based on these results, we intend to undertake in vivo studies with R. communis ethyl acetate fractions due the high effectiveness against amastigotes and promastigotes of L. infantum and the low cytotoxicity towards murine monocytic cells.

  1. Uso de cobertura com colágeno e aloe vera no tratamento de ferida isquêmica: estudo de caso El uso de colágeno y aloe vera en el tratamiento de la herida isquémica: estudio de caso Use of collagen and aloe vera in ischemic wound treatment: study case

    Directory of Open Access Journals (Sweden)

    Simone Helena dos Santos Oliveira

    2010-06-01

    Full Text Available O presente estudo relata o caso clínico de um paciente diabético e hipertenso, portador de ferida isquêmica, tratado com cobertura não convencional, à base de Aloe vera e colágeno. Faz parte de um projeto de pesquisa experimental, coordenado por professores e enfermeiros que atuam em projetos para o desenvolvimento de novas tecnologias para o tratamento de feridas. O caso em tela foi selecionado dentre os dos demais pacientes acompanhados. A coleta de dados foi efetuada através da anamnese e exame físico do paciente, utilizando-se um instrumento com dados relativos às condições do paciente e da lesão, bem como através do registro fotográfico da lesão. Os curativos foram realizados diariamente e, ao final de aproximadamente dez semanas, observou-se a total cicatrização da lesão. Não foram observados desconfortos ou complicações decorrentes do uso do produto, concluindo-se que o mesmo apresentou boa tolerabilidade e eficácia terapêutica para este caso em particular.Este estudio reporta el caso clínico de un paciente hipertenso, diabético, con una herida isquémica tratada con apósitos no convencionales de Aloe vera y colágeno, y forma parte de un proyecto de investigación experimental coordinado por profesores y enfermeros que trabajan en proyectos de desarrollo de nuevas tecnologías para el tratamiento de heridas. El caso de referencia fue elegido entre muchos pacientes en seguimiento. Los datos fueron obtenidos por la anamnesis y el examen físico del paciente, utilizándose un instrumento con datos relativos a las condiciones del paciente y de la lesión, así como un registro fotográfico de la lesión. Las curaciones se realizaron diariamente, al cabo de aproximadamente diez semanas se obtuvo la curación total. No se observó incomodidad o cualquier otra complicación derivada de la utilización del producto, por lo que se concluye en que el tratamiento demostró una buena tolerabilidad y eficacia terap

  2. Accumulation of Heavy Metals and Role of EDTA in Aloe vera L.%芦荟对土壤中重金属的吸收及EDTA影响

    Institute of Scientific and Technical Information of China (English)

    王畅; 张涛; 仇荣亮; 郭鹏然

    2012-01-01

    Distribution and effects of EDTA on accumulation of heavy metals (HM) in aloe (Aloe vera L.) were investigated by pot experiments. Results showed that HM concentrations in aloe increased with the increase of HM concentrations in soil, especially in leaves for Cd, Cu and Zn and in stems and roots for Pb. Addition of Pb to soil enhanced the concentrations of Cd and Cu in aloe, which indicated the synergistic effect of Pb and Cd, Cu. With the increase of EDTA concentration of 0-4 mmol/kg, HM concentrations in aloe increased. Translocation of HM increased due to effects of EDTA, and sequence of translocation factor is Zn, Cd, Cu and Pb. Chelating effects promoted the accumulation of HM in aloe. Accumulation of Fe in leaves of aloe was inhibited by HM, and Ca accumulation in leaves stems and roots of aloe were all inhibited by HM.%采用芦荟(Aloe vera L.)盆栽实验,考察了土壤中重金属在芦荟中的分布及施加EDTA对重金属在芦荟中积累影响.由实验结果,芦荟中重金属随土壤中重金属含量的增加而增加,Cd、Cu和Zn的增加量主要积累在叶部,而Pb的增加量主要积累在茎和根部.土壤中Pb的加入促进了芦荟对Cd和Cu的吸收,表明Pb与Cd、Cu在芦荟吸收时具有协同效应.随着土壤中EDTA用量的增加(0~4 mmol/kg),芦荟对重金属的吸收积累增加,其中Cu和Pb的增加较大.在EDTA作用下,芦荟叶部对重金属的迁移增大,其迁移系数Zn>Cd>Cu>Pb.EDTA螯合作用促使芦荟对重金属的吸收.芦荟叶部重金属对Fe的积累有明显抑制作用,而芦荟各部位中重金属对Ca的积累皆有明显抑制作用.

  3. 中华芦荟不同部位提取物抑菌活性及影响因素的研究%On Antibacterial Activity of Aloe Vera Lvar Chinensis(Haw) Berger and Affecting Factors

    Institute of Scientific and Technical Information of China (English)

    郑虹; 徐慧诠

    2014-01-01

    Antimicrobial effect of the extracts of aloe vera skin juice and aloe gel and the minimal inhibitory concentration and bacteriostasis effect of the extracts of aloe vera skin juice are studied , employing Double Layer Flat Transparent Circle Method. The results shows that the inhibition effect of aloe juice is stronger than aloe gel juice and minimal inhibitory concentration of aloe vera skin juice on 3 kinds of indicator bacteria, namely Escherichia coli, Staphylococcus aureus, Bacillus subtilis,is 0.39%; aloe vera skin juice optimum pH value is 4.5; when the pH value of aloe vera skin juice exceeds 6.5, no inhibitory activity is detected; when aloe vera skin juice is kept at 100℃for 1h, the antibacterial activity is still above 95%, which shows that aloe skin juice has strong heat stability; Aloe vera skin juice not only has good bacteriostatic effect on Gram positive and gram negative bacteria, but also restrains some fungi.%采用双层平板透明圈法研究中华芦荟凝胶与外皮提取物的抑菌效果及芦荟外皮汁的最小抑菌浓度和芦荟皮汁抑菌性的影响因素。结果表明芦荟外皮汁比芦荟凝胶汁有更强的抑菌作用;而芦荟外皮汁对大肠杆菌、金黄葡萄球菌和枯草芽孢杆菌3种指示菌的最小抑菌浓度均为3.125%;芦荟外皮汁最适作用pH为4.5,当芦荟外皮汁的pH超过6.5,完全没有抑菌活性;芦荟外皮汁在100℃下保温1h,其抑菌活性仍达95%以上,说明芦荟外皮汁具有很强的热稳定性;芦荟皮外汁不仅对革兰氏阳性菌和革兰氏阴性菌具有良好的抑菌作用,对部分真菌也具有抗性。

  4. Extraction and purification of anthraquinones derivatives from Aloe vera L. using alcohol/salt aqueous two-phase system.

    Science.gov (United States)

    Tan, Zhi-jian; Li, Fen-fang; Xu, Xue-lei

    2013-08-01

    An alcohol/salt aqueous two-phase system (ATPS) composed of 1-propanol and (NH4)2SO4 was employed to purify anthraquinones (AQs) extracted from Aloe vera L. The main influencing system parameters such as type of alcohol, type and concentration of salt, temperature and pH were investigated in detail. Under the optimal extraction conditions, AQs can be extracted into alcohol-rich phase with high extraction efficiency, meanwhile majority polysaccharides, proteins, mineral substances and other impurities were extracted into salt-rich phase. Partitioning of AQs is dependent on hydrophobic interaction, hydrogen bond interaction, and salting-out effect in ATPS. Temperature also played a great role in the partitioning. After ATPS extraction, alcohol can be recycled by evaporation; moreover, salt can be recycled by dilution crystallization method. Compared with other liquid-liquid extractions, this alcohol/salt system is much simpler, lower in cost with easier recovery of phase-forming components, which has the potential scale-up in down-processing of active ingredients in plant.

  5. Acemannan, an extracted product from Aloe vera, stimulates dental pulp cell proliferation, differentiation, mineralization, and dentin formation.

    Science.gov (United States)

    Jittapiromsak, Nawaporn; Sahawat, Dusida; Banlunara, Wijit; Sangvanich, Polkit; Thunyakitpisal, Pasutha

    2010-06-01

    This study investigated the effect of acemannan (Aloe vera gel polysaccharide) on dentin formation. Primary human dental pulp cells were treated with acemannan. New DNA synthesis, bone morphogenetic protein-2, alkaline phosphatase activity, dentin sialoprotein expression, and mineralization were determined by [(3)H]-thymidine incorporation, enzyme-linked immunosorbent assay, biochemical assay, western blotting, and Alizarin Red staining, respectively. Then the upper first molars of 24 male Sprague Dawley rats were intentionally exposed and capped with either acemannan or calcium hydroxide. At day 28, the teeth were histopathologically examined and evaluated for the degree of inflammation, dentin bridge formation, and pulp tissue organization. The results revealed that acemannan significantly increased pulp cell proliferation, bone morphogenetic protein-2, alkaline phosphatase activity, dentin sialoprotein expression, and mineralization, compared with the untreated group. The acemannan-treated group also exhibited a complete homogeneous calcified dentin bridge and good pulp tissue organization, whereas neither was detected in the calcium hydroxide-treated and sham groups. In the acemannan-treated group, either mild or no inflammation was found, whereas the other groups had various degrees of inflammation. The data suggest that acemannan promotes dentin formation by stimulating primary human dental pulp cell proliferation, differentiation, extracellular matrix formation, and mineralization. Acemannan also has pulpal biocompatibility and promotes soft tissue organization.

  6. The relationship between growth and soluble sugar concentration of Aloe vera plants grown under three levels of irradiance

    Energy Technology Data Exchange (ETDEWEB)

    Paez, A.; Gebre, G.M.; Tschaplinski, T.J. (Universidad del Zulia (Venezuela))

    1994-06-01

    The CAM plant Aloe vera was vegetatively propagated and grown under three irradiances: full sun, partial and deep shade (30% and 10% of ambient light, respectively) to determine the effect on growth, biomass allocation, and sugar concentration. After one year, the plants were harvested to determine final dry weight and the sugar concentration of the leaf mucilaginous gel. Plants grown under full sun produced twice the total dry weight of those grown under partial shade, with the difference equally partitioned between the shoot and root. Plants grown under full sun also produced thicker leaves, and more numerous and large auxiliary shoots. The dry weight of plants grown under deep shade was 8.6% that of plants grown under full sun, which was directly proportional to the irradiance received. Partial shade increased the number and length of leaves produced on the primary shoot, but the allocation of carbon to roots was the lowest of all treatments. Partial shade reduced the total sugar concentration of the leaf gel matrix to 34% that of plants under full sun, due to reductions in all sugars measured. Glucose was the most abundant soluble sugar, with its relative contribution to the total pool increasing under shade. In summary, the proportional effects of partial shading were greater on soluble sugar concentrations than on the total plant biomass produced.

  7. Survival of free and microencapsulated human-derived oral probiotic Lactobacillus paracasei SD1 in orange and aloe vera juices

    Directory of Open Access Journals (Sweden)

    Rawee Teanpaisan

    2015-06-01

    Full Text Available Microencapsulation was evaluated as a means of preserving Lactobacillus paracasei SD1, a human-derived strain with probiotic potential, in orange and aloe vera juices. The microencapsulation parameters included alginate concentration, calcium chloride concentration and hardening-time, and the efficacy of microencapsulation to preserve the survival of microencapsulated bacteria compared to free cells during exposure in fruit juices were determined. The results revealed that the viable count of free-cell form markedly decreased compared to microencapsulated form. The microencapsulation of 2% alginate (w/v and 0.05 M CaCl2 gave the best result to preserve the probiotic. It was found that viability of microencapsulated probiotic bacteria was significantly higher than free-cell in fruit juices during 8 weeks of storage time in the refrigerator. The potential probiotic trait related to inhibitory effect was not affected after microencapsulation process. In summary, the microencapsulation method may be an alternative way of preserving the viability of probiotic L. paracasei SD1.

  8. Water-soluble egg membrane enhances the immunoactivating properties of an Aloe vera-based extract of Nerium oleander leaves

    Science.gov (United States)

    Benson, Kathleen F; Newman, Robert A; Jensen, Gitte S

    2016-01-01

    Objective To evaluate a blend of two natural ingredients on immune parameters relevant for their current topical use and potential support of microcirculation in skin tissue. Materials and methods A blend (BL) of Aloe vera-based Nerium oleander extract (NAE-8i, oleandrin-free) and hydrolyzed water-soluble egg membrane (WSEM) was applied to human whole-blood cultures for 24 hours, with each separate ingredient serving as a control. Immune-cell subsets were analyzed for expression levels of the activation markers CD69 and CD25. Culture supernatants were analyzed for cytokines, chemokines, and immunoregulating peptides. Results BL increased CD69 expression on lymphocytes, monocytes, and CD3–CD56+ natural killer cells, and CD25 expression on natural killer cells. The number of CD69+CD25+ lymphocytes increased in cultures treated with BL and the separate ingredients. BL triggered production of multiple cytokines and chemokines, where CC chemokines MIP1α and MIP3α, as well as cytokines involved in wound healing – Groα, Groβ, ENA78, and fractalkine – reached levels manyfold above treatment with either NAE-8i or WSEM alone. Conclusion Data on BL showed that WSEM strongly enhanced NAE-8i’s effects on immunoactivation in vitro. This has potential relevance for support of immunity in skin tissue, including antibacterial and antiviral defense mechanisms, wrinkle reduction, and wound care. PMID:27843333

  9. In vitro study of the PLA2 inhibition and antioxidant activities of Aloe vera leaf skin extracts

    Directory of Open Access Journals (Sweden)

    Gargouri Youssef

    2011-02-01

    Full Text Available Abstract Background In the present work we determined the total phenolic content of Aloe vera leaf skin (AVLS extracts by using various solvents (hexane, chloroform-ethanol (1/1, ethyl acetate, butanol and water. We have also evaluated the antioxidant and the anti-PLA2 properties of these extracts by measuring their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA. Results The water extract exhibits the highest inhibitory effect with an IC50 = 0.22 mg/ml and interestingly no effect was observed on the digestive phospholipase A2 (group IB even at a concentration of 5 mg/ml. Antioxidant activities were also analyzed and the most active extracts were observed when using chloroform ethanol (1/1 and ethyl acetate (IC50 = 0.274 and 0.326 mg/ml, respectively. Analysis of the total phenolic content reveals that the water extract, with the best anti-PLA2 effect, was poor in phenolic molecules (2 mg GAE/g. This latter value has to be compared with the chloroform-ethanol and the ethyl acetate extracts (40 and 23.8 mg GAE/g, respectively, mostly responsible for the antioxidant activity. Conclusion A significant correlation was established between the total phenolic content and the antioxidant capacity but not with the anti PLA2 activity. Results from phytochemical screening suggest that the anti PLA2 molecules were probably catechin tannins compounds.

  10. Cálculo de la resistencia a la penetración en hojas de aloe vera (Barbadenses Miller

    Directory of Open Access Journals (Sweden)

    Giovanni Torres Charry

    2013-12-01

    Full Text Available Para caracterizar la resistencia a la penetración de hojas de aloe vera, variedad Barbadenses Miller, se diseñó una máquina y se establecieron los protocolos de medición para desplazamiento y fuerza necesarios para medir la resistencia a la penetración en tres puntos diferentes de las hojas. Los resultados mostraron que no existe una relación lineal entre el desplazamiento del indentador en la hoja y la fuerza de penetración. Por otro lado, se encontró que no existen diferencias en los valores de la fuerza de penetración al evaluar ésta en distintos puntos para una misma hoja. Se hallaron diferencias en los valores obtenidos entre hojas diferentes, lo que permite suponer que existe influencia de las características dimensionales de las hojas en el valor de la fuerza de penetración. Los valores máximos obtenidos para la fuerza de penetración variaron entre 22 y 27 N, para una velocidad de avance de la herramienta de 2.6 mm/s.

  11. Aloe vera mediated hydrothermal synthesis of reduced graphene oxide decorated ZnO nanocomposite: Luminescence and antioxidant properties

    Science.gov (United States)

    Kavyashree, D.; Nagabhushana, H.; Ananda Kumari, R.; Basavaraj, R. B.; Suresh, D.; Daruka Prasad, B.; Sharma, S. C.

    2016-05-01

    A zinc oxide/reduced graphene oxide (ZnO/rGO) nanocomposite was fabricated by facile hydrothermal route using Aloe vera gel as surfactant. The PL emission spectrum of the ZnO/rGO composite consists of four peaks at around 380, 394, 449 and 465nm. The PL intensity is found to diminish in ZnO-rGO composites rather than in pure ZnO, which was attributed to electron transfer from ZnO to rGO. A single intense glow curve was recorded in rGo-ZnO for a dose range of 1-8kGy. The TL response curve of rGO-ZnO is found to be a simple glow curve structure, linear dependence over a dose range of 1-8kGy. The obtained ZnO/rGO composite could provide a facile and eco-friendly method for the development of graphene-based nanocomposites with promising applications in radiation dosimetry and antioxidant activities.

  12. Evaluation of Anti-hyperglycaemic Action of Different Fractions and Sub-fractions from Aqueous Extract of Aloe vera Linn. Leaf on Alloxan Induced Type 2 Diabetic Rats

    Institute of Scientific and Technical Information of China (English)

    Alok Maithani; Versha Parcha; Geeta Pant; Deepak Kumar; Ishan Dhulia

    2012-01-01

    Objective: To evaluate the fasting serum glucose (FSG) lowering potential of different fractions (C & D) and subfraction (D1 & D2) from aqueous extract of Aloe vera leaf on normal and alloxan induced type 2 diabetic rats. Methods: Two fractions (C & D) obtained by common chemical treatment of the aqueous extract of Aleo vera leaf and subfraction (D1 & D2) from fraction D were administered to the alloxan induced (150mg/kg i.p.) diabetic rats. The FSG lowering capacity, of different fractions and subfractions, was then evaluated in terms of percentage reduction in blood glucose level. Results: Oral administration of fractions C & D and subfraction D1 & D2 for 15 days led significant (P<0.05) reduction to the elevated FSG level of alloxan induced diabetic rats. Percentage reduction in blood glucose level and comparison with standard drug glibenclamide suggest the superiority of fraction D and subfraction D1 in hypoglycaemic potential. Conclusions:The results suggest that fraction D and subfraction D1 from aqueous extracts of Aloe vera leaf possesses the maximum FSG lowering capacity and further investigation is required for determination of anti-diabetic principal(s) and exact mechanism of their hypoglycaemic action.

  13. 芦荟组织培养体系的建立%Study on Establishment of Tissue Culture System in Aloe vera

    Institute of Scientific and Technical Information of China (English)

    牛亚玲; 董健

    2011-01-01

    [目的] 建立一个比较合理和完善的芦荟组织培养体系,为大规模快速生产芦荟组织培养苗提供依据.[方法] 以木立芦荟和中国芦荟的叶、茎尖和根为外植体,接种于不同激素配比的MS和N6固体培养基上进行组织培养,研究不同培养基对芦荟组织培养的影响.[结果] 在不定芽诱导过程中,茎尖在培养基MS+ 6-BA 3.0 mg/L+ NAA 0.2 mg/L上的芽诱导率最高.在诱导生根过程中,以1/2MS为基本培养基,NAA浓度在0.2~0.3 mg/L时芦荟组织不定芽生根效果较好.[结论] 芦荟组培效果受到遗传基因型和外在培养条件的双重制约.%[Objective] The study aimed to establish a more reasonable and perfect tissue culture system of Aloe vera, which provided the basis for the large-scale rape production al A. Vera tissue culture seedlings. [Method] With the leaf, shoot apical and root of Aloe arborescens Mill and Aloe vera L. As the explants, they were inoculated on the MS and N6 mediums with different hormone matching to made for the tissue culture and the effects of different mediums on the tissue culture of A. Vera was studied. [Result] In the induced process of adventitious bud, the shoot apical induced on the medium of MS +6-BA 3. 0 mg/L + NAA 0. 2 mg/L got the highest bud induction rate. In the induced rooting process, with 1/2MS as the basic medium, when NAA was 0. 2-0.3 mg/L, the adventitious bud of/4, vera got better rooting effect. [Conclusion ] The effect of tissue culture of A. Vera suffered the double restriction of genetic type and the external culture conditions.

  14. Efectividad de una formulación hidrófila de Bixa orellana L. y Aloe vera L. en el tratamiento de quemaduras en terneros (Effectiveness of Bixa orellana L. and Aloe vera L. hydrophilic formulation in the treatment of burns in calves

    Directory of Open Access Journals (Sweden)

    Silveira Prado, Enrique A

    2011-01-01

    Full Text Available ResumenSe evaluó el efecto de una formulación hidrófila para el tratamiento de lasquemaduras en terneros, en que se incorporan como ingredientes activos unextracto de semillas de Bixa orellana L. y el gel de las hojas de Aloe vera L.En las condiciones del estudio, los animales tratados se recuperaron a los 4,5días promedio.SummaryThe effect of a hydrophilic formulation was evaluated for the treatment of theburns in calves which incorporates as active ingredients an extract of seeds ofBixa orellana L. and the gel of the leaves of Aloe vera L. Under the conditionsof the study, the treated animals recovered to the 4.5 days average.

  15. Chemical and physical properties of aloe vera (Aloe barbadensis Miller gel stored after high hydrostatic pressure processing Propriedades químicas e físicas do gel de aloe vera (Aloe barbadensis Miller armazenado após processamento sob alta pressão hidrostática

    Directory of Open Access Journals (Sweden)

    Karina Di Scala

    2013-03-01

    Full Text Available The aim of this study was to evaluate the influence of high hydrostatic pressure (150, 250, 350, 450, and 550 MPa, applied for 5 minutes, on antioxidant capacity, total phenolic content, color, firmness, rehydration ratio, and water holding capacity of aloe vera gel stored for 60 days at 4 °C. The analyzed properties of the pressurized gel showed significant changes after the storage period. The highest value of total phenolic content was found at 550 MPa. However, a decrease in the antioxidant capacity was observed for all pressurized gel samples when compared to the control sample (p O objetivo deste estudo foi avaliar o efeito da aplicação de altas pressões hidrostáticas (150, 250, 350, 450 e 550 MPa aplicadas durante 5 minutos sobre a atividade antioxidante, concentração de polifenóis totais, cor, firmeza, taxa de reidratação e capacidade de retenção de água do gel de aloe vera armazenado durante 60 dias a 4 °C. As propriedades analisadas do gel pressurizado mostraram mudanças significativas depois de armazenadas. Para pressões de 550 Mpa, registrou-se o maior aumento de polifenóis totais. No entanto, observou-se uma diminuição da capacidade antioxidante em todas as amostras, em comparação com a amostra não tratada (p < 0,05. As alterações de cor foram menores para pressões entre 150 e 250 MPa. A aplicação de altas pressões hidrostáticas diminuiu a firmeza do gel, registrando-se o menor valor para 150 MPa (p < 0,05. Por outro lado, o tratamento sem pressão mostrou uma maior perda de firmeza, indicando que o tratamento por altas pressões conserva esta propriedade. A aplicação de altas pressões evidenciou modificações da matriz do alimento, avaliadas como taxa de reidratação e capacidade de retenção de água.

  16. Efetividade in vitro de Aloe Vera in natura, gel de clorexidina a 0,12% e gel de clorexidina a 2% sobre Enterococccus faecalis = In vitro effectiveness of Aloe vera in natura, 0.12% chlorhexidine gel, and 2% chlorhexidine gel against Enterococccus faecalis

    Directory of Open Access Journals (Sweden)

    Semenoff, Tereza Aparecida Delle Vedove

    2008-01-01

    Full Text Available Objetivo: Comparar a ação de gel de Aloe vera in natura, gel de clorexidina a 0,12% e gel de clorexidina a 2% sobre Enterococccus faecalis. Metodologia: Utilizaram-se 40 placas Petri contendo o meio de cultura Ágar Sangue. Em 10 placas foram feitos poços no centro, com o auxílio do fundo de uma pipeta de vidro, onde foram depositados discos de papel absorvente embebidos em água destilada esterilizada (controle negativo. Em outras 10 placas, semearam-se os microrganismos Enterococccus faecalis (controle positivo. Nas 20 placas restantes, realizaram-se 4 poços nos diferentes quadrantes da placa e semearam-se os microrganismos, distribuíndo-se 4 discos umedecidos em cada substância, a saber: 1 em água destilada, 1 em gel de clorexidina a 2%, 1 em gel de clorexidina a 0,12% e 1 com gel de Aloe vera in natura. Os grupos foram analisados após 60h, comparando-se os halos de inibição de crescimento dos microrganismos. Resultados: Houve diferença estatística significativa de inibição para Enterococccus faecalis entre as substâncias, em ordem decrescente: gel de clorexidina a 2%, gel de clorexidina a 0,12%, gel de Aloe vera in natura e água destilada (P < 0,05. Conclusão: O gel de Aloe vera in natura teve efetividade antimicrobiana menor que as duas concentrações de clorexidina testadas

  17. Effect of Beeswax, Gelatin and Aloe vera Gel Coatings on Physical Properties and Shelf Life of Chicken Eggs Stored at 30°C

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    Amal Iroshan Mudannayaka

    2016-03-01

    Full Text Available Present study was to determine the effect of beeswax, gelatin and Aloe vera gel coatings on internal quality and shelf life of chicken eggs compared to uncoated and mineral oil coated eggs. Four hundred and seventy five brown shell eggs were obtained from 32 weeks old Lohmann classic brown layers and all the eggs were randomly divided into five groups as ninety five eggs per group. Mineral oil, beeswax, Aloe vera gel and gelatin coatings were applied on eggs as four treatments and one group of eggs were uncoated and kept as control group. Then all the eggs were stored at 30°C and relative humidity of 70% - 75% for six weeks of storage period. Beeswax and gelatin coated eggs showed significantly (P<0.05 lower weight loss values and preserved albumin and yolk quality of eggs than uncoated eggs. Eggs coated with mineral oil and beeswax showed similar results for weight loss, Haugh unit, yolk index, albumen and yolk pH. Based on the Haugh Unit, eggs can be classified into four grades as AA (above 72, A (72-60, B (59-31 and C (below 30. Quality of uncoated eggs, Aloe vera coated eggs and gelatin coated eggs dropped from AA to B and mineral oil and beeswax coated eggs changed from initial AA quality to A quality after six weeks of storage at 30°C. Results of microbiological analysis showed that all coated eggs were microbiologically safe throughout the storage period. The present study demonstrated that, in comparison to the mineral oil and the uncoated eggs, beeswax is a better novel coating material and gelatin can also be successfully used as coating material in preserving the internal quality and extending the shelf life of chicken eggs stored at 30 °C for six weeks.

  18. Advances in the research of promotion effect of Aloe vera on wound healing and its clinical use%芦荟促进创面愈合的机制及临床应用研究进展

    Institute of Scientific and Technical Information of China (English)

    宋信福; 陈晓东

    2016-01-01

    Aloe vera has been widely investigated and used as folk medicine since ancient time.Biologically active substances in its gel include polysaccharides,glycoprotein,enzymes,anthraquinones or phenolic compounds,vitamins,minerals,and so on,which play important roles in anti-inflammatory response,antimicrobial,antiviral,antioxidant activity,immunoregulation effects,and especially in wound healing.In this paper,we review the advances in the mechanism and clinical application of Aloe vera and its extract on wound healing,so as to provide new ideas for the treatment of various kinds of wounds.

  19. Steelhead trout Oncorhynchus mykiss metabolic rate is affected by dietary Aloe vera inclusion but not by mounting an immune response against formalin-killed Aeromonas salmonicida.

    Science.gov (United States)

    Zanuzzo, F S; Urbinati, E C; Nash, G W; Gamperl, A K

    2015-07-01

    The oxygen consumption (MO2) of two groups of 10° C acclimated steelhead trout Oncorhynchus mykiss was measured for 72 h after they were given a 100 µl kg(-1) intraperitoneal injection of formalin-killed Aeromonas salmonicida (ASAL) or phosphate-buffered saline (PBS). In addition, plasma cortisol levels were measured in fish from both groups prior to, and 1 and 3 h after, they were given a 30 s net stress. The first group was fed an unaltered commercial diet for 4 weeks, whereas the second group was fed the same diet but with 0·5% (5 g kg(-1) ) Aloe vera powder added; A. vera has potential as an immunostimulant for use in aquaculture, but its effects on basal and acute phase response (APR)-related metabolic expenditures and stress physiology, are unknown. Injection of ASAL v. PBS had no measurable effect on the MO2 of O. mykiss indicating that the APR in this species is not associated with any net increase in energy expenditure. In contrast, incorporating 0·5% A. vera powder into the feed decreased routine metabolic rate by c. 8% in both injection groups and standard metabolic rate in the ASAL-injected group (by c. 4 mg O2 kg(-1) h(-1) ; 5%). Aloe vera fed fish had resting cortisol levels that were approximately half of those in fish on the commercial diet (c. 2·5 v. 5·0 ng ml(-1) ), but neither this difference nor those post-stress reached statistical significance (P > 0·05).

  20. Inestabilidad cariológica durante la formación de células madres del polen en Aloe vera (Aloaceae)

    OpenAIRE

    Imery Buiza, José

    2007-01-01

    Con el objetivo de esclarecer la posible existencia de anomalías citogenéticas que aminoren la fertilidad del polen de Aloe vera, se analizó la etapa de proliferación celular que lleva a la formación de células madres del polen (CMPs). Se recolectaron botones florales (BF) en 25 plantas de una población ubicada a 10°34'15'' N, 64°12'08'' W, los cuales fueron fijados en Carnoy I por 24 h y almacenados en etanol (70 % v/v). Las observaciones se realizaron en preparaciones temporales obtenidas p...

  1. Distribución de metales en el acíbar de hojas de zábila (Aloe vera (L. Burm.f.

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    Carlos E. Rondón

    Full Text Available This work presents the distribution of Na, Ca, Mg, K, Cu, Zn, Mn and Fe in Aloe vera leaves exudate by flame atomic absorption spectrometry (FAAS and Cr by electrothermal atomic absorption spectrometry (ETAAS. The plants were cultivated in the city of Coro, Falcón state. The samples were collected from internal, medium and external leaves. The accuracy of the method was evaluated using a certified reference material NBS 1572. The average precision was 1.39%. The applied method was accurate, precise and free from interferences.

  2. Uso de cobertura com colágeno e aloe vera no tratamento de ferida isquêmica: estudo de caso

    OpenAIRE

    2010-01-01

    O presente estudo relata o caso clínico de um paciente diabético e hipertenso, portador de ferida isquêmica, tratado com cobertura não convencional, à base de Aloe vera e colágeno. Faz parte de um projeto de pesquisa experimental, coordenado por professores e enfermeiros que atuam em projetos para o desenvolvimento de novas tecnologias para o tratamento de feridas. O caso em tela foi selecionado dentre os dos demais pacientes acompanhados. A coleta de dados foi efetuada através da anamnese e ...

  3. Influence of drinking water containing Aloe vera (Aloe barbadensis Miller) gel on growth performance, intestinal microflora, and humoral immune responses of broilers

    Science.gov (United States)

    Shokraneh, Meisam; Ghalamkari, Gholamreza; Toghyani, Majid; Landy, Nasir

    2016-01-01

    Aim: The risk of bacteria resistance to specific antibiotics possibly by continuous subtherapeutical administration of antibiotic growth promoters (AGPs) in poultry feed led to a ban on the use of AGP in poultry production. As a result of this ban, alternative substances for poultry growth promotion and disease prevention are being investigated, among which phytogenic and herbal products have received increased attention as natural additives because they have been accepted by consumers as natural additives. The effect of water supplementation of Aloe vera (AV) as an AGP substitute on performance, intestinal microflora, and immune responses of broilers. Materials and Methods: The five experimental treatments were allocated to four replicates. The following treatments were applied (1) a basal broiler diet (C) and normal drinking water, (2) 0.5% AV gel in drinking water, (3) 0.75% AV gel in drinking water, (4) 1% AV gel in drinking water, and (5) diet C supplemented with flavophospholipol at 4.5 mg/kg and drinking normal water. Vaccines against influenza disease and sheep red blood cell (SRBC) were administrated to immunological stimuli. The populations of Lactobacilli spp. and coliforms were enumerated in ileum. Results: Body weight of broilers supplemented with different levels of AV increased compared with control group (p<0.05). Birds supplemented with antibiotic had the best feed-to-gain ratio (F:G) in different periods. Supplementation of 0.5% and 0.75% AV improved F: G entire experimental period compared with control group (p<0.05). Coliform bacteria were reduced in broilers supplemented with different levels of AV or antibiotic (p<0.05). The Lactobacilli spp. population in birds supplemented with 0.75%, 1% AV or antibiotic significantly was higher than other groups (p<0.05). Supplementation with 1% AV led to greater antibody titers against SRBC compared with other groups (p<0.05). Conclusion: These findings demonstrated a possibility of supplementing broiler

  4. Influence of drinking water containing Aloe vera (Aloe barbadensis Miller gel on growth performance, intestinal microflora, and humoral immune responses of broilers

    Directory of Open Access Journals (Sweden)

    Meisam Shokraneh

    2016-11-01

    Full Text Available Aim: The risk of bacteria resistance to specific antibiotics possibly by continuous subtherapeutical administration of antibiotic growth promoters (AGPs in poultry feed led to a ban on the use of AGP in poultry production. As a result of this ban, alternative substances for poultry growth promotion and disease prevention are being investigated, among which phytogenic and herbal products have received increased attention as natural additives because they have been accepted by consumers as natural additives. The effect of water supplementation of Aloe vera (AV as an AGP substitute on performance, intestinal microflora, and immune responses of broilers. Materials and Methods: The five experimental treatments were allocated to four replicates. The following treatments were applied (1 a basal broiler diet (C and normal drinking water, (2 0.5% AV gel in drinking water, (3 0.75% AV gel in drinking water, (4 1% AV gel in drinking water, and (5 diet C supplemented with flavophospholipol at 4.5 mg/kg and drinking normal water. Vaccines against influenza disease and sheep red blood cell (SRBC were administrated to immunological stimuli. The populations of Lactobacilli spp. and coliforms were enumerated in ileum. Results: Body weight of broilers supplemented with different levels of AV increased compared with control group (p<0.05. Birds supplemented with antibiotic had the best feed-to-gain ratio (F:G in different periods. Supplementation of 0.5% and 0.75% AV improved F:G entire experimental period compared with control group (p<0.05. Coliform bacteria were reduced in broilers supplemented with different levels of AV or antibiotic (p<0.05. The Lactobacilli spp. population in birds supplemented with 0.75%, 1% AV or antibiotic significantly was higher than other groups (p<0.05. Supplementation with 1% AV led to greater antibody titers against SRBC compared with other groups (p<0.05. Conclusion: These findings demonstrated a possibility of supplementing

  5. Water-soluble egg membrane enhances the immunoactivating properties of an Aloe vera-based extract of Nerium oleander leaves

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    Benson KF

    2016-11-01

    Full Text Available Kathleen F Benson,1 Robert A Newman,2,3 Gitte S Jensen1 1NIS Labs, Klamath Falls, OR, 2Department of Experimental Therapeutics, University of Texas MD Anderson Cancer Center, Houston, 3Nerium Biotechnology Inc, San Antonio, TX, USA Objective: To evaluate a blend of two natural ingredients on immune parameters relevant for their current topical use and potential support of microcirculation in skin tissue. Materials and methods: A blend (BL of Aloe vera-based Nerium oleander extract (NAE-8i, oleandrin-free and hydrolyzed water-soluble egg membrane (WSEM was applied to human whole-blood cultures for 24 hours, with each separate ingredient serving as a control. Immune-cell subsets were analyzed for expression levels of the activation markers CD69 and CD25. Culture supernatants were analyzed for cytokines, chemokines, and immunoregulating peptides. Results: BL increased CD69 expression on lymphocytes, monocytes, and CD3–CD56+ natural killer cells, and CD25 expression on natural killer cells. The number of CD69+CD25+ lymphocytes increased in cultures treated with BL and the separate ingredients. BL triggered production of multiple cytokines and chemokines, where CC chemokines MIP1α and MIP3α, as well as cytokines involved in wound healing – Groα, Groβ, ENA78, and fractalkine – reached levels manyfold above treatment with either NAE-8i or WSEM alone. Conclusion: Data on BL showed that WSEM strongly enhanced NAE-8i’s effects on immunoactivation in vitro. This has potential relevance for support of immunity in skin tissue, including antibacterial and antiviral defense mechanisms, wrinkle reduction, and wound care. Keywords: chemokines, cytokines, leukocyte activation

  6. Facile one step synthesis of novel TiO2 nanocoral by sol-gel method using Aloe vera plant extract

    Science.gov (United States)

    Venkatesh, K. S.; Krishnamoorthi, S. R.; Palani, N. S.; Thirumal, V.; Jose, Sujin P.; Wang, Fu-Ming; Ilangovan, R.

    2015-05-01

    Titanium oxide (TiO2) nanoparticles (NPs) were synthesized by sol gel method using Aloe vera plant extract as a biological capping agent and a cauliflower-nanocoral morphology was observed in this technique. The assynthesized TiO2 nanopowder was calcined at a range of temperatures (300-600 °C) for 1 h. The influence of A. vera plant extract on the thermal, structural and morphological properties of TiO2 nanopowder was evaluated. Thermogravimetric analysis/differential thermal analysis was employed to study the thermal properties of the assynthesized TiO2 nanopowder. The crystallinity, phase transformation and the crystallite size of the calcined samples were studied by X-ray diffraction technique. XRD result confirmed the presence of TiO2 with anatase phase. FT Raman spectra showed the Raman active modes pertaining to the TiO2 anatase phase and Raman band shift was also observed with respect to particle size variation. The different functional group vibrations of as dried pure A. vera plant extract were compared with the mixture of TiO2 and A. vera plant extract by FT-IR analysis. The scanning electron microscopy images apparently showed the formation of spherical shaped NPs and also it demonstrated the effect of A. vera plant extract on the reduction of particles size. The surface area of the TiO2 NPs was measured through Brunauer-Emmett-Teller analysis. Transmission electron microscopy images ascertained that the spherical shaped TiO2 NPs were formed with cauliflower-nanocoral morphology decorated with nanopolyps with the size range between 15 and 30 nm.

  7. Effect of acemannan, an extracted polysaccharide from Aloe vera, on BMSCs proliferation, differentiation, extracellular matrix synthesis, mineralization, and bone formation in a tooth extraction model.

    Science.gov (United States)

    Boonyagul, Sani; Banlunara, Wijit; Sangvanich, Polkit; Thunyakitpisal, Pasutha

    2014-07-01

    Aloe vera is a traditional wound healing medicine. We hypothesized acemannan, a polysaccharide extracted from Aloe vera gel, could affect bone formation. Primary rat bone marrow stromal cells (BMSCs) were treated with various concentrations of acemannan. New DNA synthesis, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein, osteopontin expression, and mineralization were determined by [(3)H] thymidine incorporation assay, ELISA, biochemical assay, western blotting, and Alizarin Red staining, respectively. In an animal study, mandibular right incisors of male Sprague-Dawley rats were extracted and an acemannan treated sponge was placed in the socket. After 1, 2, and 4 weeks, the mandibles were dissected. Bone formation was evaluated by dual-energy X-ray absorptiometry and histopathological examination. The in vitro results revealed acemannan significantly increased BMSC proliferation, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein and osteopontin expression, and mineralization. In-vivo results showed acemannan-treated groups had higher bone mineral density and faster bone healing compared with untreated controls. A substantial ingrowth of bone trabeculae was observed in acemannan-treated groups. These data suggest acemannan could function as a bioactive molecule inducing bone formation by stimulating BMSCs proliferation, differentiation into osteoblasts, and extracellular matrix synthesis. Acemannan could be a candidate natural biomaterial for bone regeneration.

  8. Enriched adhesion of talc/ZnO nanocomposites on cotton fabric assisted by aloe-vera for bio-medical application

    Science.gov (United States)

    Selvakumar, D.; Thenammai, A. N.; Yogamalar, N. R.; Hemamalini, R.; Jayavel, R.

    2015-06-01

    Synthesis and characterization of talc/ZnO nanocomposites with the assistance of aloe-vera are investigated by structural and morphological studies. The crystal structure and the phase analysis of ZnO and talc are characterized and confirmed by X-ray diffraction (XRD) analysis. The average crystallite size estimation from the Scherrer formula and the particle size analysis clearly predicts that the size of the ZnO declines when aloe-vera is used as a capping molecule in comparison to the commercially available ZnO. The reduced crystallite size of ZnO renders a stable cohesion with the talc composition and the presence of distinct functional group pyridines/ammonia in the synthesized nanocomposites enriches the good adhesion between the as-synthesized material and cotton fabric. The adhesion and homogeneous distribution of talc/ZnO nanocomposites on the cotton fabric are inferred from the scanning electron microscopy (SEM) results. The basic studies and characterizations would pave way for futuristic bio-medical application.

  9. Enriched adhesion of talc/ZnO nanocomposites on cotton fabric assisted by aloe-vera for bio-medical application

    Energy Technology Data Exchange (ETDEWEB)

    Selvakumar, D.; Yogamalar, N. R.; Jayavel, R., E-mail: rjvel@annauniv.edu [Centre for Nanoscience and Technology, Anna University, Chennai – 600025 (India); Thenammai, A. N.; Hemamalini, R. [Department of Physics, Queen Mary’s College, Chennai – 600004 (India)

    2015-06-24

    Synthesis and characterization of talc/ZnO nanocomposites with the assistance of aloe-vera are investigated by structural and morphological studies. The crystal structure and the phase analysis of ZnO and talc are characterized and confirmed by X-ray diffraction (XRD) analysis. The average crystallite size estimation from the Scherrer formula and the particle size analysis clearly predicts that the size of the ZnO declines when aloe-vera is used as a capping molecule in comparison to the commercially available ZnO. The reduced crystallite size of ZnO renders a stable cohesion with the talc composition and the presence of distinct functional group pyridines/ammonia in the synthesized nanocomposites enriches the good adhesion between the as-synthesized material and cotton fabric. The adhesion and homogeneous distribution of talc/ZnO nanocomposites on the cotton fabric are inferred from the scanning electron microscopy (SEM) results. The basic studies and characterizations would pave way for futuristic bio-medical application.

  10. Exploring the potential of gastro retentive dosage form in delivery of ellagic acid and aloe vera gel powder for treatment of gastric ulcers.

    Science.gov (United States)

    Ranade, Arati N; Ranpise, Nisharani S; Ramesh, C

    2014-01-01

    Approach of novel drug delivery system (NDDS) overcomes the limitations of conventional dosage forms. However, this concept is still not practiced to a large extent in delivery of herbal drugs in Ayurveda. Thus, the potential of herbal drugs has not been explored to its fullest. Hence, there is a growing need to amalgamate the concept of NDDS in delivery of herbal constituents. The present investigation is designed to deliver and retain two herbal constituents in stomach for better action against Helicobacter pylori induced gastric ulcers. The objective was to develop a bilayer floating tablet of ellagic acid and Aloe vera gel powder through rational combination of excipients to give the lowest possible lag time with maximum drug release in the period of 4 h. Formulation F9 containing 100 mg of HPMC K15M, 27 mg of crospovidone, 80 mg of mannitol and effervescent agents in the ratio 1:2 gave 92% drug release and desired floating properties. In vivo studies showed that combination of ellagic acid and Aloe vera gave 75 % ulcer inhibition in comparison to 57% ulcer inhibition in the group which was administered with ellagic acid alone. This suggests the use of bilayer floating tablet in gastric ulcer treatment.

  11. Excavating the Role of Aloe Vera Wrapped Mesoporous Hydroxyapatite Frame Ornamentation in Newly Architectured Polyurethane Scaffolds for Osteogenesis and Guided Bone Regeneration with Microbial Protection.

    Science.gov (United States)

    Selvakumar, M; Pawar, Harpreet Singh; Francis, Nimmy K; Das, Bodhisatwa; Dhara, Santanu; Chattopadhyay, Santanu

    2016-03-09

    Guided bone regeneration (GBR) scaffolds are unsuccessful in many clinical applications due to a high incidence of postoperative infection. The objective of this work is to fabricate GBR with an anti-infective electrospun scaffold by ornamenting segmented polyurethane (SPU) with two-dimensional Aloe vera wrapped mesoporous hydroxyapatite (Al-mHA) nanorods. The antimicrobial characteristic of the scaffold has been retrieved from the prepared Al-mHA frame with high aspect ratio (∼14.2) via biosynthesis route using Aloe vera (Aloe barbadensis miller) extract. The Al-mHA frame was introduced into an unprecedented SPU matrix (solution polymerized) based on combinatorial soft segments of poly(ε-caprolactone) (PCL), poly(ethylene carbonate) (PEC), and poly(dimethylsiloxane) (PDMS), by an in situ technique followed by electrospinning to fabricate scaffolds. For comparison, pristine mHA nanorods are also ornamented into it. An enzymatic ring-opening polymerization technique was adapted to synthesize soft segment of (PCL-PEC-b-PDMS). Structure elucidation of the synthesized polymers is established by nuclear magnetic resonance spectroscopy. Sparingly, Al-mHA ornamented scaffolds exhibit tremendous improvement (175%) in the mechanical properties with promising antimicrobial activity against various human pathogens. After confirmation of high osteoconductivity, improved biodegradation, and excellent biocompatibility against osteoblast-like MG63 cells (in vitro), the scaffolds were implanted in rabbits as an animal model by subcutaneous and intraosseous (tibial) sites. Improved in vivo biocompatibilities, biodegradation, osteoconductivity, and the ability to provide an adequate biomimetic environment for biomineralization for GBR of the scaffolds (SPU and ornamented SPUs) have been found from the various histological sections. Early cartilage formation, endochondral ossification, and rapid bone healing at 4 weeks were found in the defects filled with Al-mHA ornamented

  12. Efficacy of passive ultrasonic irrigation with natural irrigants (Morinda citrifolia juice, Aloe Vera and Propolis in comparison with 1% sodium hypochlorite for removal of E. faecalis biofilm: An in vitro study

    Directory of Open Access Journals (Sweden)

    Anuj Bhardwaj

    2013-01-01

    Full Text Available Aim: Present study evaluated the efficacy of natural derivative irrigants, Morinda citrifolia juice (MCJ, Aloe Vera and Propolis in comparison to 1% sodium hypochlorite with passive ultrasonic irrigation for removal of the intraradicular E. faecalis biofilms in extracted single rooted human permanent teeth. Materials and Methods: Biofilms of E. faecalis were grown on the prepared root canal walls of 60 standardized root halves which were longitudinally sectioned. These root halves were re-approximated and the samples were divided into five groups of twelve each. The groups were, Group A (1% NaOCl, Group B (MCJ, Group C (Aloe vera, Group D (Propolis and Group E (Saline. These groups were treated with passive ultrasonic irrigation (PUI along with the respective irrigants. The root halves were processed for scanning electron microscopy. Three images (X2.5, coronal, middle and apical, were taken for the twelve root halves in each of the five groups. The images were randomized and biofilm coverage assessed independently by three calibrated examiners, using a four-point scoring system. Results: 1% NaOCl with passive ultrasonic irrigation (PUI was effective in completely removing E. faecalis biofilm and was superior to the natural irrigants like MCJ, Aloe vera and Propolis tested in this study. Conclusion: 1% NaOCl used along with passive ultrasonic irrigation was effective in completely removing E. faecalis biofilm when compared to natural irrigants (MCJ, Aloe Vera and Propolis.

  13. Comparative evaluation of antimicrobial effect of herbal root canal irrigants (Morinda citrifolia, Azadirachta indica, Aloe vera) with sodium hypochlorite: An in vitro study

    Science.gov (United States)

    Babaji, Prashant; Jagtap, Kiran; Lau, Himani; Bansal, Nandita; Thajuraj, S.; Sondhi, Priti

    2016-01-01

    Aim and Objectives: Successful root canal treatment involves the complete elimination of microorganism from the root canal and the three-dimensional obturation of the canal space. Enterococcus faecalis is the most commonly found bacteria in failed root canal. Chemical irrigation of canals along with biomechanical preparation helps in the elimination of microorganisms. The present study was aimed to evaluate the antimicrobial effect of herbal root canal irrigants (Morinda citrifolia, Azadirachta indica extract, Aloe vera) with sodium hypochlorite (NaOCl). Materials and Methods: The bacterial E. faecalis (ATCC) culture was grown overnight in brain heart infusion (BHI) broth and inoculated in Mueller–Hinton agar plates. Antibacterial inhibition was assessed using agar well diffusion method. All five study irrigants were added to respective wells in agar plates and incubated at 37°C for 24 h. Bacterial inhibition zone around each well was recorded. Results were tabulated and statistically analyzed using Statistical Package for the Social Sciences software for Windows, version 19.0. (IBM Corp., Armonk, NY. Results: Highest inhibitory zone against E. faecalis was seen in NaOCl fallowed by M. citrifolia and A. indica extract, and the least by A. vera extract. Conclusion: Tested herbal medicine (A. indica extract, M. citrifolia, A. vera) showed inhibitory zone against E. faecalis. Hence, these irrigants can be used as root canal irrigating solutions. PMID:27382533

  14. Study on effects of lead on the antioxidant activities of Aloe vera%铅污染对芦荟抗氧化活性影响的研究

    Institute of Scientific and Technical Information of China (English)

    蔡卓; 卢登峰; 梁信源; 莫创荣; 杜良伟; 莫利书; 黄富嵘

    2012-01-01

    The variation of the antioxidant enzyme system of Aloe vera under the stress of different Pb concentrations was studied using pot experiments. The scavenging abilities of the potted Aloe vera to ·OH and O2- o were determined by flow injection chemiluminescence method using methylene blue-Fenton system and 1,2,3 -trihydroxybenzene system. The effects of lead pollution on the enzymatic activity of Aloe vera were evaluated. The results showed that at a relatively low lead pollution level, the enzymatic activity of polluted Aloe vera was higher than that of the un-polluted, and increased with the increase of lead concentration in Aloe vera body; while at a relatively higher lead pollution level, the enzymatic activity of polluted Aloe vera was lower than that of the un-polluted, and the activity decreased with the increase of lead concentration in Aloe vera body. These indicated that at a low pollution level the physiological response of Aloe vera antioxidant enzyme system to heavy metal stress resulted in the rise of enzymatic activity of Aloe vera; while at a high pollution level, the self protection system of Aloe vera is damaged, which in turn led to the damage of antioxidant enzyme system, and then results in the decrease of the enzymatic activity.%采用盆栽试验,对芦荟在不同浓度Pb胁迫下抗氧化酶系统的变化进行了研究.用流动注射化学发光分析法,以亚甲基蓝-Fenton体系、鲁米诺-邻苯三酚体系分别测定盆栽芦荟体内抗氧化酶清除·OH和清除O2-·的能力,进而评价铅污染对芦荟生物活性的影响.结果表明,在相对低浓度铅污染水平下,芦荟提取酶的活性均比无污染时的对照值有所升高,并与芦荟体内铅含量成正相关;而在相对较高浓度铅污染下,提取酶的活性均比对照值有所下降,与芦荟体内铅含量成负相关.说明在低浓度铅毒害作用下,芦荟体内抗氧化酶系统对重金属胁迫的生理的响应使其活性有所上升

  15. Removal of reactive dyes from aqueous solutions by a non-conventional and low cost agricultural waste: adsorption on ash of Aloe Vera plant

    Directory of Open Access Journals (Sweden)

    Mohammad Malakootian

    2014-08-01

    Full Text Available Dyes are an important class of pollutants and disposal of them in precious water resources must be avoided. Among various methods adsorption occupies a prominent place in dye removal. The aim of this study is to evaluate adsorption of dye Reactive Red 198 and Blue 19 (RR-198 & RB-19 (on to Aloe Vera plant ash from aqueous solutions. In this research Aloe Vera ash was prepared at laboratory conditions and then after shredding, screened by ASTM standard sieve with 60 -200 mesh sizes and the effects of pH (3-12, adsorbent dose (0.1-1 g/L, contact time (10-60 min, initial dye concentration (10-160 mg/L and temperature were investigated in the experiment. In different samples Dye concentration was measured by spectrophotometer at 592 nm and 520 nm wavelength for RR198 and RB19 respectively. Also the Langmuir and Freundlich adsorption isotherms were determined in order to describe the relations between the colored solutions and the adsorbent. The results of this study showed that acidic conditions were more conducive to enhance the hydrolysis rate than basic ones as the decomposition was optimum at pH 3. The adsorption rate of RR-198 and RB-19 dyes was increased by increasing of initial dye concentration, increasing of adsorbent dose in 0.1 to 0.4 mg/L. Dye solution was decolorized in a relatively short time (20 min. The efficiencies for RR-198 and RB- 19 reactive dyes were 82.68% and 90.42% respectively. The maximum adsorption capacity (qmax has been found to be 80.152 mg/g for RR-198 reactive dye and 88.452 mg/g for Blue 19 reactive dye. Adsorption isotherms were examined by Freundlich and Langmuir isotherm that finally showed the Freundlich multilayer isotherm has better accordance with dates. The results indicate that Aloe Vera ash plant as a natural and inexpensive adsorbent is a suitable adsorbent for the adsorption of textile dyes.

  16. Antioxidant, anti-inflammatory, anti-apoptotic, and skin regenerative properties of an Aloe vera-based extract of Nerium oleander leaves (NAE-8®

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    Benson KF

    2015-05-01

    Full Text Available Kathleen F Benson,1 Robert A Newman,2,3 Gitte S Jensen1 1NIS Labs, Klamath Falls, Oregon, USA; 2University of Texas MD Anderson Cancer Center, Houston, TX, USA; 3Nerium Biotechnology, Inc, San Antonio, TX, USA Objective: The goal for this study was to evaluate the effects of an Aloe vera-based Nerium oleander extract (NAE-8®, compared to an extract of A. vera gel alone (ALOE, and to an aqueous extract of N. oleander (AQ-NOE in bioassays pertaining to dermatologic potential with respect to antioxidant protection, anti-inflammatory effects, and cytokine profiles in vitro. Methods: Cellular antioxidant protection was evaluated in three separate bioassays: The cellular antioxidant protection of erythrocytes (CAP-e assay, protection of cellular viability and prevention of apoptosis, and protection of intracellular reduced glutathione levels, where the last two assays were performed using human primary dermal fibroblasts. Reduction of intracellular formation of reactive oxygen species (ROS was tested using polymorphonuclear cells in the absence and presence of oxidative stress. Changes to cytokine and chemokine profiles when whole blood cells and human primary dermal fibroblasts were exposed to test products were determined using a 40-plex Luminex array as a method for exploring the potential cross-talk between circulating and skin-resident cells. Results: The NAE-8® provided significantly better antioxidant protection in the CAP-e bioassay than AQ-NOE. NAE-8® and AQ-NOE both protected cellular viability and intracellular reduced glutathione, and reduced the ROS formation significantly when compared to control cells, both under inflamed and neutral culture conditions. ALOE showed minimal effect in these bioassays. In contrast to the NAE-8®, the AQ-NOE showed induction of inflammation in the whole blood cultures, as evidenced by the high induction of CD69 expression and secretion of a number of inflammatory cytokines. The treatment of dermal

  17. 芦荟芽苗快繁及试管苗栽培驯化研究%A Study on the Rapid Regeneration and Plantlet Cultivation Under in Vitro Culture of Aloe (Aloe vera L.var, Chinensis (haw) Berger)

    Institute of Scientific and Technical Information of China (English)

    杜勇军; 李惠芝; 祁云枝

    2003-01-01

    以中华斑纹芦荟(Aloe vera L. var.chinensis(Haw.)Berger)为试验材料,研究了芦荟的组织培养、快速繁殖和试管苗驯化及栽培技术.结果表明,短缩茎诱导愈伤组织效果较好.芽苗快速繁殖阶段以MS+6-BA2.0十NAA1.0+Su50g/L为最佳;试管苗栽培驯化中基质以珍珠岩:感石:砂子为1:1:2最佳;活性炭对防止根变态有重要作用.

  18. Effect of an extract of Aloe vera on the biodistribution of sodium pertechnetate (Na99mTcO4 in rats Efeito de um extrato de Aloe vera na biodistribuição do pertecnetato de sódio (Na99mTcO4 em ratos

    Directory of Open Access Journals (Sweden)

    Cecília Maria de Carvalho Xavier Holanda

    2009-10-01

    Full Text Available PURPOSE: Aloe vera is a tropical plant popularly known in Brazil as babosa. We have investigated the effect of aqueous extract of Aloe vera on the biodistribution of Na99mTcO4 and laboratorial parameters in Wistar rats. METHODS: Twelve animals were divided into treated and control groups. In the treated group, Aloe vera was given by gavage (5mg/mL/day during 10 days. The control group received sorbitol by the same way and period. One hour after the last dose, we injected 0.1mL of Na99mTcO4 by orbital plexus. After 60 min, all the animals were killed. Samples were harvested from the brain, liver, heart, muscle, pancreas, stomach, femur, kidneys, blood, testis and thyroid and the percentage of radioactivity (%ATI/g was determined. Biochemical dosages were performed. RESULTS: There was a significant increase of %ATI/g in blood, femur, kidneys, liver, stomach, testis and thyroid and also in blood levels of AST and ALT. A significant decrease in levels of glucose, cholesterol, triglycerides, creatinine and urea occurred. The statistical analyses were performed by Mann-Whitney test and T-Student test (pOBJETIVO: Aloe vera é uma planta tropical popularmente conhecida no Brasil por "babosa". Investigou-se o efeito de extrato aquoso do A. vera na biodistribuição do pertecnetato de sódio (Na99mTcO4 e em parâmetros laboratoriais de ratos Wistar. MÉTODOS: Doze animais foram divididos em 2 grupos: tratado e controle. No grupo tratado, o extrato de A. vera foi administrado via oral (5mg/mL/dia por 10 dias. O grupo controle recebeu sorbitol do mesmo modo. Uma hora após a última dose, ambos receberam 0,1mL de Na99mTcO4 via plexo orbital. Após 60 minutos, os animais foram sacrificados. Foram retiradas amostras do cérebro, fígado, coração, músculo, pâncreas, estômago, fêmur, rins, sangue, testículos e tiróide e determinou-se o percentual de radioatividade por grama (%ATI/g de cada uma. Dosagens bioquímicas foram realizadas. RESULTADOS: Houve

  19. KARYOTYPE ANALYSIS OF CHROMOSOMES OF ALOE VERA[L.] BURM. F%库拉索芦荟染色体核型分析

    Institute of Scientific and Technical Information of China (English)

    张明浩; 崔丽华; 陈正华

    2005-01-01

    对库拉索芦荟(Aloe vera[L.]Burm.f)细胞染色体进行了核型分析,染色体类型按Levan方法分类.研究表明,库拉索芦荟染色体数为2n=14.染色体的核型为K(2n)=2x=6sm+6st+2m.其中第2,3,4对染色体为亚端部着丝粒染色体,第1,5,6对染色体为亚中部着丝粒染色体,第7对染色体为中部着丝粒染色体,在所有7对染色体中未观察到有携带随体的染色体存在.

  20. CONDUCTANCIA ESTOMÁTICA Y ASIMILACIÓN NETA DE CO2 EN SÁBILA (Aloe vera Tourn) BAJO SEQUÍA

    OpenAIRE

    Juan Patishtán Pérez; Raúl Rodríguez García; Francisco Zavala García; Diana Jasso Cantú

    2010-01-01

    La sábila (Aloe vera Tourn) tiene importancia económica relevante en las industrias farmacéutica, de cosméticos y alimentaria. Esta planta posee el metabolismo fotosintético ácido de las crasuláceas (MAC). En México se cultiva en diversas regiones con clima subtropical. El objetivo de este trabajo fue comparar el intercambio de gases de sábila en condiciones de riego y de sequía, en dos localidades mexicanas, una con clima semiseco cálido (Marín, Nuevo León) y otra con clima semiárido templad...

  1. Mosquitocidal and antibacterial activity of green-synthesized silver nanoparticles from Aloe vera extracts: towards an effective tool against the malaria vector Anopheles stephensi?

    Science.gov (United States)

    Dinesh, Devakumar; Murugan, Kadarkarai; Madhiyazhagan, Pari; Panneerselvam, Chellasamy; Kumar, Palanisamy Mahesh; Nicoletti, Marcello; Jiang, Wei; Benelli, Giovanni; Chandramohan, Balamurugan; Suresh, Udaiyan

    2015-04-01

    Mosquitoes represent an important threat for lives of millions of people worldwide, acting as vectors for devastating pathogens, such as malaria, yellow fever, dengue, and West Nile. In addition, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. Here, we investigated the mosquitocidal and antibacterial properties of Aloe vera leaf extract and silver nanoparticles synthesized using A. vera extract. Mosquitocidal properties were assessed in laboratory against larvae (I-IV instar) and pupae of the malaria vector Anopheles stephensi. Green-synthesized silver nanoparticles were tested against An. stephensi also in field conditions. Antibacterial properties of nanoparticles were evaluated against Bacillus subtilis, Klebsiella pneumoniae, and Salmonella typhi using the agar disk diffusion and minimum inhibitory concentration protocol. The synthesized silver nanoparticles were characterized by UV-vis spectrum, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD). In laboratory conditions, the A. vera extract was toxic against An. stephensi larvae and pupae, even at low dosages. LC50 were 48.79 ppm (I instar), 59.09 ppm (II instar), 70.88 ppm (III instar), 83.58 ppm (IV instar), and 152.55 ppm (pupae). Green-synthesized silver nanoparticles were highly toxic against An. stephensi. LC50 were 3.825 ppm (I instar), 4.119 ppm (II instar), 4.982 ppm (III instar), 5.711 ppm (IV instar), and 6.113 ppm (pupae). In field conditions, the application of A. vera-synthesized silver nanoparticles (10 × LC50) leads to An. stephensi larval reduction of 74.5, 86.6, and 97.7%, after 24, 48, and 72 h, respectively. Nanoparticles also showed antibacterial properties, and the maximum concentration tested (150 mg/L) evoked an inhibition zone wider than 80 mm in all tested bacterium species. This study adds knowledge about the use of green synthesis of nanoparticles in

  2. 不同温度下芦荟皮厌氧发酵产沼气的实验研究%Experimental Study on the Anaerobic Fermentation of the Aloe Vera Peel under Different Temperatures

    Institute of Scientific and Technical Information of China (English)

    郭德芳; 李秋敏; 刘丽春; 张无敌; 尹芳; 刘士清; 陈玉保; 赵兴玲; 柳静

    2013-01-01

    为获得不同温度条件下芦荟皮厌氧发酵产沼气的潜力和特性,采用全混合批量发酵装置分别在20℃和30℃下对其进行沼气发酵实验.结果表明,在20℃时,芦荟皮发酵净产气量为952mL,TS产气率为349mL/g,VS产气率为423mL/g;在30℃时,净产气量为1 303mL,TS产气率为478mL/g,VS产气率为579mL/g,说明芦荟皮具有较好的产气潜力;30℃条件下芦荟皮的TS产气率为20℃条件下的1.37倍,TS利用率、VS利用率都比20℃条件下的高1倍以上,表明30℃更利于芦荟皮厌氧发酵产沼气.%In order to obtain biogas production potential and production characteristics of the aloe vera peel under different temperatures,this study took aloe vera peel as raw material and using batch fermentation device in the temperature 30℃ and 20℃ respectively.The results indicated that the net biogas production of the Aloe vera peel is 952mL at 20℃,and TS gas production rate is 349mL/g,VS gas production rate is 423mL/g; the net biogas production is 1 303mL at 30℃,and TS gas production rate is 478mL/g,VS gas production rate is 579mL/g.These results suggests that the Aloe vera peel has good biogas production potential.The TS gas production rate of Aloe vera peel at 30℃ is 1.37 times at temperature 20℃,TS utilization rate and VS utilization rate at 30℃ is more than 1 time at 20℃,indicating that the temperature 30℃ is more conducive to theAloe Vera peel anaerobic fermentation.

  3. 几种芦荟萌蘖比较及上农大叶生物量配置%Tillering Comparison of Several Species of Aloes and the Biomass Distribution of Aloe vera L.var. SCA Gu

    Institute of Scientific and Technical Information of China (English)

    费永俊; 胡莉莉; 张玲慧; 白玉芳

    2002-01-01

    对长江中联公司种植的库拉索芦荟(Aloe vera L.)、木立芦荟(A.arbotescens Mill)、上农大叶芦荟(A.vera.L.var.SCA Gu)萌蘖数、2年生库拉索组培苗和根生苗萌蘖数进行了比较,同时研究了上农大叶及库拉索2类萌蘖对母株生物量的影响,结果表明:木立芦荟多在老叶叶腋处萌芽,系数9.2;上农大叶与库拉索之间萌蘖能力无显著差异;库拉索芦荟2年生组培苗与根生苗萌蘖数无差异,生物量相近,均重分别为1.6375kg和1.4625kg,即生长速度相近;上农大叶萌蘖明显影响植株的营养生长,一般萌蘖数越多,母株生物量越少.

  4. Comparative evaluation of antimicrobial activity of hydroalcoholic extract of Aloe vera, garlic, and 5% sodium hypochlorite as root canal irrigants against Enterococcus faecalis: An in vitro study

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    Swati Ramesh Karkare

    2015-01-01

    Full Text Available Introduction: Enterococcus faecalis are the most resistant and predominant microorganisms recovered from root canals of teeth where previous treatment has failed. Over the past decade, interest in drugs derived from medicinal plants has markedly increased. In dentistry, phytomedicines has been used as an anti-inflammatory, antibiotic, analgesic, sedative, and also as an endodontic irrigant. In endodontics, because of the cytotoxic reactions of most of the commercial intracanal medicaments and their inability to eliminate bacteria completely from dentinal tubules, the trend is shifting toward use of biologic medication extracted from natural plants. Aim: To compare the antimicrobial efficacy of newer irrigating agents which would probably be as effective or more and at the same time less irritating to the tissues than sodium hypochlorite (NaOCl. The objective of this study was to compare the antimicrobial activity of saturated and diluted (1:1 hydroalcoholic extract of Aloe vera, garlic, and 5% NaOCl against E. faecalis using the commonly used agar diffusion method. Results: Saturated hydroalcoholic extract of A. vera showed the highest zone of inhibition against E. faecalis. NaOCl, which is considered as gold standard, also showed higher zones of inhibition.

  5. Oral administration of Aloe vera gel powder prevents UVB-induced decrease in skin elasticity via suppression of overexpression of MMPs in hairless mice.

    Science.gov (United States)

    Saito, Marie; Tanaka, Miyuki; Misawa, Eriko; Yao, Ruiquing; Nabeshima, Kazumi; Yamauchi, Kouji; Abe, Fumiaki; Yamamoto, Yuki; Furukawa, Fukumi

    2016-07-01

    This study reports the effects of oral Aloe vera gel powder (AVGP) containing Aloe sterols on skin elasticity and the extracellular matrix in ultraviolet B (UVB)-irradiated hairless mice. Ten-week-old hairless mice were fed diets containing 0.3% AVGP for 8 weeks and irradiated UVB for 6 weeks. Mice treated with AVGP showed significant prevention of the UVB-induced decrease in skin elasticity. To investigate the mechanism underlying this suppression of skin elasticity loss, we measured the expression of matrix metalloproteinase (MMP)-2, -9, and -13. AVGP prevented both the UVB-induced increases in MMPs expressions. Moreover, we investigated hyaluronic acid (HA) content of mice dorsal skin and gene expression of HA synthase-2 (Has2). In the results, AVGP oral administration prevented UVB-induced decreasing in skin HA content and Has2 expression and attenuates the UVB-induced decrease in serum adiponectin, which promotes Has2 expression. These results suggested that AVGP has the ability to prevent the skin photoaging.

  6. Avaliação da atividade mutagênica e antimutagênica da Aloe vera em teste de Allium cepa e teste de micronúcleo em linfócitos humanos binucleados

    Directory of Open Access Journals (Sweden)

    Régis T. Sturbelle

    2010-07-01

    Full Text Available Algumas das substâncias presentes nos vegetais podem ter efeitos mutagênicos, enquanto outras podem atenuar ou anular estes efeitos. Estima-se que mais de 200 substâncias ativas façam parte do gel de Aloe vera, sendo que os polissacarídeos chegam a 30%, e muitos dos benefícios terapêuticos, nutricionais e cosméticos são a eles atribuídos. O objetivo deste trabalho foi determinar o efeito mutagênico e antimutagênico da solução de Aloe vera, em teste de Allium cepa e teste de micronúcleos em linfócitos humanos binucleados. Para as soluções, as folhas de Aloe vera foram trituradas, filtradas e esterilizadas. O teste de Allium cepa foi constituído de oito tratamentos e o de linfócitos, de cinco (com quatro repetições. Observou-se que na dose usual, a solução de Aloe vera não foi mutagência para o sistema de teste vegetal e nem para o humano. Já na dose dez vezes mais concentrada provocou um efeito citotóxico e mutagênico em Allium cepa. Em células vegetais a solução foi antimutagênica apenas quando colocada depois do paracetamol, enquanto que nas células humanas, essa ação se manifestou quando a solução foi utilizada ao mesmo tempo com o paracetamol.

  7. Evaluation of Antioxidation and Whitening Activities for Combination of Aloe vera and Angelica dahurica Extracts%芦荟、白芷提取物联合应用的抗氧化和美白活性评估

    Institute of Scientific and Technical Information of China (English)

    黄立森; 张瞳昕; 庞海月; 吴黉坦; 叶子坚; 王贵弘

    2016-01-01

    为评估芦荟和白芷的乙醇提取物联合应用在抗氧化和美白方面的协同增效作用,采用乙醇浸提法从芦荟和白芷中提取分离功效成分,应用DPPH和ABTS方法评估其抗氧化活性,酪氨酸酶活性抑制实验评估其美白活性。结果表明,芦荟和白芷提取物联合应用比单独应用具有更强的抗氧化、清除DPPH自由基和抑制酪氨酸酶活性。芦荟和白芷提取物联合应用在抗氧化和美白方面具有协同增效作用。%To evaluate the synergetic effect of antioxidation and whitening activities for combination of Aloe vera and Angelica dahurica extracts,the active ingredients from Aloe vera and Angelica dahurica were separated by an ethanol ex-traction method. The antioxidant activity was evaluated by DPPH and ABTS methods. And the whitening activity was test-ed by using a tyrosinase inhibition experiment. Results showed that,the combination of Aloe vera and Angelica dahurica extracts had stronger antioxidant activity than the individual,as well as in the aspect of DPPH free radical scavenging and inhibiting tyrosinase activities. It has significant synergetic effect of antioxidation and whitening activities for combination of Aloe vera and Angelica dahurica extracts.

  8. Oral Administration of Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) and Honey Improves the Host Body Composition and Modulates Proteolysis Through Reduction of Tumor Progression and Oxidative Stress in Rats.

    Science.gov (United States)

    Tomasin, Rebeka; de Andrade, Rafael Siqueira; Gomes-Marcondes, Maria Cristina Cintra

    2015-10-01

    Oxidative stress has a dual role in cancer; it is linked with tumorigenic events and host wasting, as well as senescence and apoptosis. Researchers have demonstrated the importance of coadjuvant therapies in cancer treatment, and Aloe vera and honey have immunomodulatory, anticancer, and antioxidant properties. The preventive and therapeutic effects of Aloe vera (L.) Burm. f. (Xanthorrhoeaceae) and honey in tumor progression and host wasting were analyzed in Walker 256 carcinoma-bearing rats. The animals were distributed into the following groups: C=control-untreated, W=tumor-untreated, WA=treated after tumor induction, A=control-treated, AW=treated before tumor induction, and AWA=treated before and after tumor induction. Proteolysis and oxidative stress were analyzed in the tumor, liver, muscle, and myocardial tissues. The results suggest that the Aloe vera and honey treatment affect the tumor and host by different mechanisms; the treatment-modulated host wasting and cachexia, whereas it promoted oxidative stress and damage in tumor tissues, particularly in a therapeutic context (WA).

  9. Comparative evaluation of anti-obesity effect of Aloe vera and Gymnema sylvestre supplementation in high-fat diet fed C57BL/6J mice

    Science.gov (United States)

    Pothuraju, Ramesh; Sharma, Raj Kumar; Rather, Sarver Ahmed; Singh, Satvinder

    2016-01-01

    Background: The aim of the present study was to investigate, anti-obesity effect of Aloe vera (AV), and Gymnema sylvestre (GS) whole extract powders administration to high-fat diet (HFD) fed C57BL/6J mice for 12 weeks. Materials and Methods: At the end of experiment, different parameters such as body weight, feed intake, organ weights, fasting blood glucose, oral glucose tolerance test, plasma lipid levels, and expression analysis of adipocytokines were evaluated. Results: At the end of experimental period, oral administration of both herbs showed a significant (P < 0.05 and P < 0.001) decrease in the plasma glucose and lipid levels in HFD fed mice. In addition, increased in the epididymal fat (E. fat) weight in the HFD group was significantly (P < 0.05) reduced on GS administration alone. Finally, quantitative mRNA expression analysis of adiponectin gene was significantly up-regulated in AV supplementation. Further, no effect was observed with the both herbs on pro-inflammatory cytokines (interleukin 6 and tumor necrosis factor-a) in the E. fat tissue of HFD fed group. Conclusions: The anti-obesity and other metabolic studies depend on the type of diet, different parts of herbal extractions, and animal models used. Further studies are required in this area to strengthen the anti-obesity effects of herbs with active component, and it can be used a pro-drug instead of whole extract. PMID:27757271

  10. EXAFS analysis of cations distribution in structure of Co1-xNixFe2O4 nanoparticles obtained by hydrothermal method in aloe vera extract solution

    Science.gov (United States)

    Wongpratat, Unchista; Maensiri, Santi; Swatsitang, Ekaphan

    2016-09-01

    Effect of cations distribution upon EXAFS analysis on magnetic properties of Co1-xNixFe2O4 (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co2+, Ni2+ ions of different ionic radii at both tetrahedral and octahedral sites in the crystal structure. Particle sizes of samples estimated by TEM images were found to be in the range of 10.87-62.50 nm. The VSM results at room temperature indicated the ferrimagnetic behavior of all samples. Superparamagnetic behavior was observed in NiFe2O4 sample. The coercivity (Hc) and remanance (Mr) values were related to the particle sizes of samples. The saturation magnetization (Ms) was increased by a factor of 1.4 to a value of 57.57 emu/g, whereas the coercivity (Hc) was decreased by a factor of 20 to a value of 63.15 Oe for a sample with x = 0.75. In addition to the cations distribution, the increase of aspect ratio (surface to volume ratio) due to the decrease of particle size could significantly affect the magnetic properties of the materials.

  11. 丛枝菌根在芦荟育苗中的应用%Study on Application of Arbuscular-Mycorrhizas in Growing Seedlings of Aloe vera

    Institute of Scientific and Technical Information of China (English)

    弓明钦; 王凤珍; 陈羽

    2002-01-01

    利用6种丛枝(AM)菌和 1 种 AM 菌剂对库拉索芦荟Aloe vera 组培幼苗接种的结果表明:供试菌种或菌剂均可使幼苗形成丛枝菌根,感染率达96.67%~100%,感染指数达73.30~86.67;接种13个月的苗高比对照增加19.85%~51.91%;叶片长度比对照增加56.67%;芦荟幼苗经接种后,叶片汁液的干物质含量,比对照分别增加13.13%~150.%%.接种15个月的芦荟幼苗叶汁鲜重比对照增加60.87%~233.80%;折合有效成分的生药含量,比对照提高2.17倍~7.24倍.

  12. Evaluation of Aloe vera and synbiotic as antibiotic growth promoter substitutions on performance, gut morphology, immune responses and blood constitutes of broiler chickens.

    Science.gov (United States)

    Naghi Shokri, Ali; Ghasemi, Hossein A; Taherpour, Kamran

    2017-02-01

    This study was performed to investigate the effect of dietary supplementation with Aloe vera (AV) powder and synbiotic as growth promoter agents on performance, gut morphology, immune responses, hematology and serum biochemistry in broilers. A total of 240-day-old male broiler chicks (Ross 308) were randomly assigned to six treatments with four replicates. Birds were offered either a corn-soybean meal basal diet (control) or the basal diet supplemented with 200 mg/kg virginiamycin (VM), 1 g/kg synbiotic (Syn), 2.5 g/kg AV (AV1), 5.0 g/kg AV (AV2) or 7.5 g/kg AV (AV3). Chickens fed any of the diets, except diet AV1, exhibited better feed conversion ratios at the 14-28 day period and higher average daily gain and duodenal villus height/crypt depth ratio at 42 days than those fed the control diet. Synbiotic supplementation caused a marked increase in the serum antibody titer against infectious bursal disease and infectious bronchitis vaccines. Feeding diet AV3 significantly increased red blood cell count and hemoglobin concentration, and decreased serum triglyceride level compared to the control group. The results suggested that dietary inclusion of 5 and 7.5 g/kg AV, similar with synbiotic supplementation, can be applied as effective alternatives to in-feed antibiotics for broiler diets.

  13. Tissue Culture and Rapid Rooting of Aloe vera var.chinensis%库拉索芦荟的组织培养和植株快速生根

    Institute of Scientific and Technical Information of China (English)

    那淑芝; 张东豪; 甄占轩

    2003-01-01

    @@ 1 植物名称库拉索芦荟(Aloe vera var. chinensis). 2 材料类别顶芽或无木质化的茎段. 3 培养条件芽诱导培养基:(1)MS+6-BA 2 mg*L-1(单位下同)+NAA 0.2.不定芽增殖、分化培养基:(2)MS+6-BA 1+NAA 0.1~0.2.快速生根培养基:(3)1/10大量元素(MS其它含量不变)+NAA 0.2.以上培养基均含0.7%琼脂,pH 5.8,培养基(1)、(2)含3%蔗糖,培养基(3)含2%蔗糖.培养温度20~27℃,光照10~12 h*d-1,光照度1 500 lx.

  14. Novel buccal adhesive tablets using Aloe vera L and Sinapis alba--a promising option for improved bioavailability of diltiazem hydrochloride.

    Science.gov (United States)

    Sudhakar, Yajaman; Bandyopadhyay, A K

    2008-01-01

    In the current investigation, white mustard mucilage from whole seeds of Sinapis alba was evaluated for its physical properties and compared with the other mucoadhesive polymers such as hydroxy propyl methylcellulose 5Cps and Carbopol 934P. Further, methanol precipitable solids from whole leaves of Aloe Vera L were used as permeation enhancer. To achieve improved bioavailability of diltiazem, novel buccal adhesive tablets (NBATs) in cup and core fashion designed to achieve unidirectional release towards mucosa were prepared in a three-stage process using specially fabricated punches. The adhesive cups were studied for its shear, tensile, and peel strengths by specially designed apparatus using excised ruminant and porcine buccal mucosa as model substrates. Ex vivo permeation studies in a Franz diffusion cell were conducted through porcine buccal mucosa. Fourier transform infrared spectroscopy studies and differential scanning calorimetry thermographs showed no remarkable interactions. Histopathological studies showed no remarkable damage of buccal mucosa by the NBATs. In vivo studies were conducted on anaesthetized male New Zealand albino rabbits, estimated by reversed-phase high-performance liquid chromatography, and the pharmacokinetics were compared with the oral and intravenous bolus injection. NBATs exhibited a Cmax 74.6 ng/mL, Tmax 3.5 h, t(1/2) 4.36 h. The NBATs prevented salivary scavenging effect and exhibited 82.1% bioavailability.

  15. 芦荟凝胶治疗Ⅱ期压疮的效果研究%Effect study on treating stage Ⅱ pressure sore by aloe vera gel

    Institute of Scientific and Technical Information of China (English)

    王银花; 江华容

    2015-01-01

    Objective To investigate the clinical effect of aloe vera gel in the treatment of stage Ⅱ .Method 60 ca‐ses with bedsore at stage Ⅱ were selected and computer randomly divided into experimental group and control group ,each group had 30 patients .two groups were given the same conventional nursing care:turn over on time , provide enough nutrition ,keep the skin clean and dry etc .Aloe vera gel as observation group ,mepilex as control group ,Outcomes ,healing time ,epithelial tissue growth after 3 ,5 ,7 days were observed .Result The two group in the outcome had no statistical significance (P> 0 .05);The recovery time of the observation group was significantly shorter than the control group(P0 .05);and the drug cost of observation group was signifi‐cantly lower than the control group (P< 0 .01) .Conclusion when the aloe gel and mepilex border have the same treatment effect ,The former which have a low cost ,can be used to the treatment of stage Ⅱ bedsore ,Especially the pantients of stage Ⅱ bedsore in the community and family .%目的:探讨芦荟凝胶治疗Ⅱ期压疮的临床效果。方法将62例Ⅱ期压疮患者按计算机数字表法随机分为观察组和对照组,每组各31例。两组患者均给予相同的常规护理:按时翻身,加强营养,保持皮肤清洁干燥等措施相同。观察组采用芦荟凝胶护理压疮,对照组采用美皮康敷料护理压疮,观察两组转归,痊愈时间,第3天、第5天、第7天上皮组织生长及药物费用。结果两组患者压疮转归方面差异无统计学意义(P>0.05);痊愈时间观察组明显短于对照组(P<0.01);上皮组织的生长方面第3天、第5天具有统计学意义(P<0.01),第7天无统计学意义(P >0.05);观察组的药物费用明显低于对照组(P <0.01)。结论芦荟凝胶对Ⅱ期压疮的治疗效果不亚于美皮康,但成本低于美皮康,尤其适合推广于社区、家庭Ⅱ期压疮的治疗。

  16. 芦荟及其复方提取物对RAW264.7细胞炎症因子的影响%Effects of Aloe vera and Compound Extract of Aloe vera on Inflammatory Factors in RAW264.7 Cells

    Institute of Scientific and Technical Information of China (English)

    潘苗苗; 刘学华; 佟书娟; 詹臻; 董伟

    2012-01-01

    目的:观察芦荟及其复方提取物对小鼠巨噬细胞炎症因子的影响.方法:RAW264.7细胞按2×105/mL稀释,100μL/孔接种入96孔板,药物质量浓度为250,125,62.5 g?L-1下采用噻唑蓝比色法(MTT法)作用4h,检测芦荟及其复方提取物对小鼠巨噬细胞的毒性作用;药物质量浓度为50,25,12.5 g?L-1下采用硝酸还原酶法作用24 h,测定小鼠巨噬细胞一氧化氮(N0)含量,并用酶联免疫吸附法(ELISA法)测定其在单纯药物及脂多糖(LPS)1 mg?L-1诱导情况下小鼠巨噬细胞肿瘤坏死因子-α(TNF-α)、白介素-10(IL-10)的含量.结果:芦荟及其复方提取物具有促进巨噬细胞释放NO的作用,并与药物浓度成正相关(P<0.01);芦荟有促进TNF-α分泌(P<0.01)和抑制IL-10分泌(P<0.05)的作用,其复方在LPS诱导下则对TNF-α和IL-10都有抑制作用(P<0.01).结论:芦荟在250~7.8 g?L-1下对臣噬细胞无细胞毒性,其复方则有一定毒性;芦荟及其复方对小鼠巨噬细胞炎症因子的释放有一定影响.%Objective: To observe effect of the Aloe vera and its compound extract on inflammatory factors. Method: Cells diluted by 2 × 10 /mL were inoculated into 96-well plates, 100 μL Per well, concentrations of druds was 250, 125, 62.5 g ? L-1 , MTT method was assay to test the toxic effects of macrophages. When the concentrations was 50, 25, 12. 5 g ? L-1 macrophage nitric oxide (NO) levels was measured. Enzyme-linked immunosorbent assay ( ELISA) were used to measure tumor necrosis factor-α ( TNF-α) and interleukin-10 ( IL-10) levels in pure drug and lipopolysaccharide ( LPS) -induced murine macrophage.Result: Aloe vera and its compound extract promoted releasing of NO, with a concentration dependent manner ( P < 0. 01) ; Aloe vera could promote TNF-α secretion ( P < 0. 01 ) and inhibition of IL-10 secretion ( P < 0. 05 ) , the compound inhibited LPS induced releasing of TNF-α and IL-10 (P <0. 01). Conclusion; Aloe in 250-7. 8 g ? L-1

  17. 芦荟胶治疗小分子靶向药物所致药疹的疗效观察%Effect of aloe vera gel therapy on drug eruption of patients with oral small molecule targeted drugs

    Institute of Scientific and Technical Information of China (English)

    张艳玲; 刘东英; 王丽

    2013-01-01

    Objective To investigate the efficiency of aloe vera gel therapy on drug eruption of non small cell lung cancer(NSCL) patients taken oral small molecule targeted drugs.Methods Twentysix NSCLC patients with drug eruption who accepted the therapy of small molecule targeted drugs were selected.The patients were randomized into the treatment group and the control group.The patients in treatment group were given aloe vera gel to daub skin rash place,while the patients in control group were given Mupirocin Ointment.Results The effect of treatment group is superior to the control group.And the difference has significant difference.Conclusions Aloe vera gel is an effective drug for eruption of NSCLC patients taken oral small molecule targeted drugs.%目的 探讨芦荟胶外涂治疗非小细胞肺癌患者口服小分子靶向药物所致药疹的效果.方法 选择26例接受小分子靶向药物治疗致药疹的非小细胞肺癌患者进行随机分组作对照研究.治疗组应用芦荟胶涂抹皮疹处,对照组应用莫匹罗星.结果 治疗组药疹治疗效果明显优于对照组,差异有统计学意义.结论 芦荟胶可有效治疗非小细胞肺癌患者小分子靶向药物所致药疹.

  18. 芦荟胶对放、化疗所致骨髓抑制保护作用的研究%Protective effect of aloe vera gel on bone marrow suppression caused by radiotherapy and chemotherapy

    Institute of Scientific and Technical Information of China (English)

    马秀龙; 邓怀慈; 刘小平

    2013-01-01

    目的:观察芦荟胶对放、化疗所致骨髓抑制的保护作用及其发生机制.方法:普通SD大鼠96只随机分为实验组与对照组.实验组放、化疗前均以芦荟胶灌胃,对照组用生理盐水灌胃.放、化疗后取血、骨髓,检测外周血WBC、RBC、PLT计数,血清IL-3、GM-CSF水平以及骨髓单个核细胞凋亡率.结果:用芦荟胶灌胃组外周血WBC计数明显高于生理盐水灌胃组,差别有统计学意义(P<0.05);用芦荟胶灌胃组骨髓单个核细胞凋亡率明显低于生理盐水灌胃组,差别有统计学意义(P<0.05).而RBC、PLT计数及血清IL-3、GM-CSF含量差别无统计学意义(P>0.05).结论:芦荟胶对放、化疗导致的骨髓抑制具有保护作用.%Objective:To investigate the protective effect of aloe vera gel for chemoradiotherapy-induced myelosuppression,and its mechanism.Methods:All 96 normal SD rats were randomly divided into experimental and control groups.Aloe vera gel was used before chemoradiotherapy orally for experimental group and the control group with saline.To detect peripheral blood WBC,RBC,PLT count,serum IL-3,GM-CSF levels and bone marrow mononuclear cells apoptosis rate after chemoradiotherapy.Results:In aloe vera gel orally group,peripheral WBC count was significandy higher than the saline group,the difference was statistically significant (P < 0.05).Bone marrow mononuclear cell apoptosis rate of experimental group was significantly lower than the saline group,the difference was statistically significant (P < 0.05).While difference of RBC,PLT count and serum IL-3 and GM-CSF content was not statistically significant (P > 0.05).Conclusion:Aloe vera gel has a protective effect on radiotherapy and chemotherapy caused bone marrow suppression.

  19. Preliminary Studies on Endophytic Fungi in Aloe vera L. var. chinensis in the North of Guangdong Province%粤北地区中华芦荟内生真菌的初步研究

    Institute of Scientific and Technical Information of China (English)

    柯野; 方白玉; 曾松荣

    2007-01-01

    从中华芦荟[Aloe vera L. var. chinensis (Haw.) Berger]的根和叶中分离出内生真菌40株,经形态学鉴定属于3目3科7属;分别对其液体发酵液进行抗菌活性检测,发现青霉属LH4和头孢属LH34菌株具有抗菌活性.

  20. Evaluation of in-vitro cytotoxicity and cellular uptake efficiency of zidovudine-loaded solid lipid nanoparticles modified with Aloe Vera in glioma cells

    Energy Technology Data Exchange (ETDEWEB)

    Joshy, K.S. [Department of Chemistry, CMS College Kottayam, Kerala (India); International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam 686 560, Kerala (India); Sharma, Chandra P. [Division of Biosurface Technology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Poojappura, Thiruvananthapuram, Kerala (India); Kalarikkal, Nandakumar [International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam 686 560, Kerala (India); School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam 686 560, Kerala (India); Sandeep, K. [International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam 686 560, Kerala (India); Thomas, Sabu, E-mail: sabuchathukulam@yahoo.co.uk [International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam 686 560, Kerala (India); School of Chemical Sciences, Mahatma Gandhi University, Kottayam 686 560, Kerala (India); Pothen, Laly A. [Department of Chemistry, Bishop Moore College, Mavelikkara, Kerala (India)

    2016-09-01

    Zidovudine loaded solid lipid nanoparticles of stearic acid modified with Aloe Vera (AV) have been prepared via simple emulsion solvent evaporation method which showed excellent stability at room temperature and refrigerated condition. The nanoparticles were examined by Fourier transform infrared spectroscopy (FT-IR), which revealed the overlap of the AV absorption peak with the absorption peak of modified stearic acid nanoparticles. The inclusion of AV to stearic acid decreased the crystallinity and improved the hydrophilicity of lipid nanoparticles and thereby improved the drug loading efficacy of lipid nanoparticles. Dynamic light scattering (DLS) and transmission electron microscopy (TEM) imaging revealed that, the average particle size of unmodified (bare) nanoparticles was 45.66 ± 12.22 nm and modified solid lipid nanoparticles showed an average size of 265.61 ± 80.44 nm. Solid lipid nanoparticles with well-defined morphology were tested in vitro for their possible application in drug delivery. Cell culture studies using C6 glioma cells on the nanoparticles showed enhanced growth and proliferation of cells without exhibiting any toxicity. In addition, normal cell morphology and improved uptake were observed by fluorescence microscopy images of rhodamine labeled modified solid lipid nanoparticles compared with unmodified nanoparticles. The cellular uptake study suggested that these nanoparticles could be a promising drug delivery system to enhance the uptake of antiviral drug by brain cells and it could be a suitable drug carrier system for the treatment of HIV. - Highlights: • SLN of AZT-SA, AZT-SA-AV was developed • Better drug loading efficacy • Good uptake.

  1. Aloe vera Plant Extracted Green Synthesis, Structural and Opto-Magnetic Characterizations of Spinel Co(x)Zn₁₋xAl₂O₄Nano-Catalysts.

    Science.gov (United States)

    Manikandan, A; Durka, M; Selvi, M Amutha; Antony, S Arul

    2016-01-01

    Spinel Co(x)Zn₁₋xAl₂O₄ (0 ≤ x ≤ 1) nano-catalysts were synthesized by a simple Aloe vera plant extracted green synthesis route. Powder XRD patterns and Rietveld analysis confirmed the formation of single phase, cubic spinel gahnite structure without other impurities. The lattice parameter increased from 8.089 to 8.125 A with increasing CO²⁺ content. The average crystallite sizes were estimated using Scherrer's method, and it was found to be in the range of 15.72 nm to 26.53 nm. FT-IR spectra showed vibrational stretching frequencies corresponding to the spinel structure. HR-SEM and HR-TEM images showed the features of well particle shaped crystals with nano-sized grains. The elemental compositions of Co, Zn, Al and O were quantitatively obtained from EDX analysis. The band gap energy estimated using Kubelka-Munk method by UV-Visible DRS method, and the values are decreased with increasing the Co²⁺ content (4.12 eV to 3.67 eV), due to the formation of sub bands in between the energy gap. PL spectra showed emission bands in UV as well as in the visible regions for ZnAl₂O₄ and Co-doped ZnAl₂O4, due to the defect centers acting as the trap levels. VSM measurements revealed that pure ZnAl₂O₄ has diamagnetic, while Co doped ZnAl₂O₄ samples (x = 0.2 to 0.8) have superparamagnetism, whereas the sample CoAl₂O₄ has ferromagnetic in nature. Catalytic oxidation of benzyl alcohol to benzaldehyde was found that the sample Co₀.₆Zn₀.₄Al₂O₄ showed 93.25% conversion with 99.56% selectivity, whereas for pure ZnAl₂O₄, the conversion was only 86.31% with 92.85% selectivity.

  2. A simple aloe vera plant-extracted microwave and conventional combustion synthesis: Morphological, optical, magnetic and catalytic properties of CoFe2O4 nanostructures

    Science.gov (United States)

    Manikandan, A.; Sridhar, R.; Arul Antony, S.; Ramakrishna, Seeram

    2014-11-01

    Nanocrystalline magnetic spinel CoFe2O4 was synthesized by a simple microwave combustion method (MCM) using ferric nitrate, cobalt nitrate and Aloe vera plant extracted solution. For the comparative study, it was also prepared by a conventional combustion method (CCM). Powder X-ray diffraction, energy dispersive X-ray and selected-area electron diffraction results indicate that the as-synthesized samples have only single-phase spinel structure with high crystallinity and without the presence of other phase impurities. The crystal structure and morphology of the powders were revealed by high resolution scanning electron microscopy and transmission electron microscopy, show that the MCM products of CoFe2O4 samples contain sphere-like nanoparticles (SNPs), whereas the CCM method of samples consist of flake-like nanoplatelets (FNPs). The band gap of the samples was determined by UV-Visible diffuse reflectance and photoluminescence spectroscopy. The magnetization (Ms) results showed a ferromagnetic behavior of the CoFe2O4 nanostructures. The Ms value of CoFe2O4-SNPs is higher i.e. 77.62 emu/g than CoFe2O4-FNPs (25.46 emu/g). The higher Ms value of the sample suggest that the MCM technique is suitable for preparing high quality nanostructures for magnetic applications. Both the samples were successfully tested as catalysts for the conversion of benzyl alcohol. The resulting spinel ferrites were highly selective for the oxidation of benzyl alcohol and exhibit important difference among their activities. It was found that CoFe2O4-SNPs catalyst show the best performance, whereby 99.5% selectivity of benzaldehyde was achieved at close to 93.2% conversion.

  3. In-Vitro Culture and Rapid Propagation of Aloe vera L.Var.chinensis(Haw.)Berger%中国芦荟离体培养和快速繁殖

    Institute of Scientific and Technical Information of China (English)

    贺红; 肖省娥; 徐鸿华; 刘春来; 方斌斌; 梁丽仪

    2001-01-01

    【目的】探讨采用植物组织培养技术进行中国芦荟的快速繁殖,为工厂化育苗提供技术依据。【方法】以中国芦荟地下茎发生的幼芽为外植体,诱导丛生芽的产生。【结果】基本培养基选择MT(MurashigeandTucker)较好;培养基中附加6-苄基氨基嘌呤(6-Benzylaminopurine,BA)能提高成芽率,并能增加外植体出芽的数量;BA浓度为2mg·L-1时,有利于芽的诱导与生长,出芽率达100%;根的诱导,以MT+0.5mg·L-1萘乙酸(Naphthaleneacid,NAA)培养基较好,生根率为100%。【结论】通过培养芦荟地下茎发生的幼芽,既可以迅速建立快速繁殖体系,又能使优良品种的种性得以保持。%【Objective】To find out technological evidence for the rapid propagation of Aloe vera L.var.chinensis (Haw.)Berber.【 Methods】The technique of pl ant tissue culture was used to study the rapid propagation of Aloe vera L.Var.Chinesis(Haw.)Berger.The shoot growing from the underground stem of Aloe vera L.V ar.chinensis(Haw.)Berger was taken as the explant to induce fascic ular bud.【Res ults】The optimal culture medium was MT.MT culture medium added with 6-benzylami nopurine(BA) was effe ctive to induce the shoot formation and increase the direct shooting rate.The op timal BA concentration for the induction and formation of shoot was 2 mg·L-1,the shooting rate being 100%.As for the induction of rooting,MT culture medi um adding with 0.5 mg·L-1naphthalene acid was optimal,the rooting rate being 100%.【Conc lusion】The culture of Aloe shoot growing from the underground stem,prop er culture medium and BA concentration are important for the rapid propagation of Aloe.

  4. GEL DE ALOE-VERA COMO POTENCIAL INHIBIDOR DE LA CORROSIÓN DEL ACERO DE REFUERZO ESTRUCTURAL

    Directory of Open Access Journals (Sweden)

    Héctor Herrera-Hernández

    2015-01-01

    Full Text Available En el presente trabajo se estudiaelectroquímicamente un GELextracto de las hojas de ALOE VERAcomo un posible inhibidor de la corrosión del acero de refuerzo estructuraldel concreto.Los estudiosde corrosiónse llevaron a cabo en 1M de HCl y utilizando la técnica de espectroscopia de impedancia electroquímica (EISse evaluó la resistencia a la corrosión en presencia y ausencia del GEL.Los diagramas de impedancia mostraronque la adición en diferentes proporcionesdel GELinhibe notablemente el proceso de la corrosión del acero inmerso en la solución ácida. También se determinó que las moléculas del GEL obedecena un mecanismo de fisisorciónsobre la superficie del metal de acuerdo con el modelo de isoterma de Langmuircon unG°adsde alrededor de 14.17 kJ/mol. El GEL se clasificócomo un inhibidor orgánico del tipo mixto.

  5. Membrana de poli (L-CO-D, L ÁCIDO LÁTICO-CO-TMC (PLDLA-TMC (70/30 com aloe vera para ser usado como curativo no tratamento de queimadura

    Directory of Open Access Journals (Sweden)

    Newton Maciel de Oliveira

    2016-10-01

    Full Text Available Ao longo da história, muitos tratamentos diferentes e, por vezes bizarros, têm sido prescritos para queimaduras. Desde os tempos mais remotos até os dias atuais, existe a necessidade de buscar novas terapias e drogas que funcionem como agentes tópicos para aliviar a dor e controlar a infecção em queimaduras. Dessa maneira, uma alternativa interessante às drogas sintéticas é a utilização de plantas, como um recurso natural, as quais podem ser úteis para o desenvolvimento de novas formulações e estratégias para melhorar a qualidade de vida. Neste trabalho foi utilizado o terpolímero poli (L-co-D, L ácido lático-co-TMC (PLDLA-co-TMC como matriz polimérica para a fabricação do curativo, sendo misturado a esse curativo o extrato de Aloe Vera, uma planta tropical que pertence à família Liliaceae, sendo conhecida por ser a mais antiga planta medicinal na natureza, a qual exibe propriedades anti-cancerígena, anti-oxidante, anti-bacteriana, cicatrizante e anti-viral. Além disso, outra vantagem do uso destes materiais está relacionada ao custo, uma vez que os produtos atualmente disponíveis para o tratamento de queimaduras são importados e apresentam valor elevado, inviabilizando o uso em larga escala. Neste trabalho, o estudo in vivo foi realizado por meio de 30 animais (ratos Wistar, divididos em 02 grupos conforme o tempo dos curativos (7 dias e 15 dias. Cada um, por sua vez, foi subdividido em 03 grupos de acordo com o tratamento aplicado: um grupo controle (sem a membrana PLDLA-co-TMC, tratamento 1 (com a membrana PLDLA-co-TMC sem Aloe Vera e tratamento 2 (com a membrana PLDLA-co-TMC/Aloe Vera com concentração de 10%.

  6. Use of Aloe vera shell ash supported Ni0.5Zn0.5Fe2O4 magnetic nanoparticles for removal of Pb (II from aqueous solutions

    Directory of Open Access Journals (Sweden)

    Samira Namavari

    2016-03-01

    Full Text Available Background: Lead (Pb is a heavy metal that is widely utilized in industries. It contaminates soil and groundwater. Its non-biodegradability, severe toxicity, carcinogenicity, ability to accumulate in nature and contaminate groundwater and surface water make this toxic heavy metal extremely dangerous to living beings and the environment. Therefore, technical and economic methods of removing Pb are of great importance. This study evaluated the efficiency of Ni0.5Zn0.5Fe2O4 magnetic nanoparticles supported by Aloe vera shell ash in removing Pb from aqueous environments. Methods: The adsorbent was characterized by several methods, including x-ray diffraction (XRD, scanning electron microscopy (SEM, and Fourier transform infrared spectroscopy (FT-IR. Then, the potential of Aloe vera shell ash-supported Ni0.5Zn0.5Fe2O4 magnetic nanoparticles to adsorb Pb (II was investigated. To determine the amount of lead absorbed by this adsorbent, different pHs (2, 4, 5, and 6, adsorbent doses (0.01-0.40 g, Pb concentrations (5, 10, 20, 30, 40, 50, 60, 80, 100, 200, 300, and 600 mg/L, and exposure times (0, 5, 10, 15, 20, 30, 40, 50, and 60 minutes until reaching equilibrium were tested using an atomic absorption spectrometer (Varian-AA240FS. Residual concentrations of Pb were read. Results: The results show that a time of 15 minutes, pH value of 9, and adsorbent dose of 0.2 g are the optimum conditions for Pb (II removal by this adsorption process. Increasing the initial concentration of Pb (II from 5 to 600 mg/L decreased removal efficiency from 98.8% to 73%. The experimental data fit well into the Freundlich isotherm model (R2 = 0.989. Conclusion: Ni0.5Zn0.5Fe2O4 magnetic nanoparticles supported by Aloe vera shell ash comprise a low-cost, simple, and environmentally benign procedure. The maximum monolayer adsorption capacity based on the Langmuir isotherm (R2 = 0.884 is 47.2 mg g-1. The prepared magnetic adsorbent can be well dispersed in aqueous solutions and

  7. Study on Aloe Vera L.Var.Chinensis Proliferation in Vitro%中华芦荟离体快速繁殖技术体系的研究

    Institute of Scientific and Technical Information of China (English)

    王国莉; 陈韵然

    2010-01-01

    以中华芦荟(Aloe vera L.var.chinensis)的叶片、顶芽、茎段和根为外植体进行离体快速繁殖技术体系研究,探讨了影响其快速繁殖的因素.结果显示,茎段为最适外植体,从株高为15 cm 的芦荟植株中,切取长为1 cm 的茎段,以正放置接种方式诱导不定芽效果最好,最佳诱导培养基为MS+6-BA2.0 mg/L+IAA2.0 mg/L,增殖系数为15.6.

  8. A preliminary study on the induction and propagation of adventitious buds for Aloe vera L.%库拉索芦荟诱导与增殖的初步研究

    Institute of Scientific and Technical Information of China (English)

    杜文平; 石大兴; 徐利远; 余桂容; 王米力

    2004-01-01

    本试验采用库拉索芦荟(Aloe vera L.)幼嫩茎段、茎尖、叶片、根茎为外植体进行芽的诱导与增殖实验.结果表明幼嫩茎段是芦荟组培的最适外植体,改良H培养基是芽诱导的最适基本培养基.丛芽在继代培养中用MS+BA 2 mg/ L+NAA 0.01 mg/ L,其月增殖系数可达到4.17,最佳继代周期为35 d,其年理论增殖系数可达1.59×106.

  9. Effects and mechanism of aloe vera extracts on control of botrytis in postharvest apples%芦荟粗提物对苹果采后灰霉病的防治效果与机理

    Institute of Scientific and Technical Information of China (English)

    袁仲玉; 周会玲; 田蓉; 张晓晓; 潘钰雪

    2014-01-01

    Currently, there is an increasing interest in the use of Aloe Vera in the food industry, with it being used as a source of functional foods in drinks, beverages, and ice creams. In addition, there are several reports about the antifungal activity of Aloe Vera on human mycological diseases. However, little evidence exists on the effect of Aloe extracts on fruit postharvest diseases caused by fungi, either ex-vivo or in vitro. In recent years, there have been some reports on the effect of Aloe extracts applied at postharvest in controlling fruit spoilage by fungi. However, no in-depth information has been reported on the role of Aloe extracts in decay development in fruits previously inoculated with fungi responsible for postharvest diseases or defense-related enzyme activities and modulation of antioxidant system activities. Thus, the objective of the present work was to evaluate the protective effects of Aloe extracts on Red Fuji apple fruit decay caused by Botrytis cinerea, as well as the effect of Aloe extracts on the disease resistance ability in Red Fuji apple fruit. In order to search for an alternative to synthetic fungicides for the control of the postharvest decay of Red Fuji apple fruits, the potential of using Aloe extract flavonoid for the control of postharvest gray mold incidence of Red Fuji apple fruits was investigated. Red Fuji apple fruits were disinfected with 75%(v/v) ethanol for 2 min., rinsed with tap water, air-dried, and then divided randomly into four treatment groups, with 140 fruit in each group. Three groups were dipped into 0.020, 0.035 and 0.050 g/mL Aloe extract flavonoid solutions for 5 min. at room temperature (23±1)℃. Another group (the control) was treated with distilled water. A uniform wound (4mm deep×3 mm wide) was made at the equator of each fruit using a sterile dissecting needle, followed by the inoculation of a20μL conidial suspension of B.cinerea (1×108spores/mL) into each wound site. Inoculated fruit were placed in

  10. 不同盐度胁迫对芦荟生长和离子吸收分配的影响%Effects of Salt Concentration on Growth, Ion Absorption and Distribution of Aloe vera L.

    Institute of Scientific and Technical Information of China (English)

    马艳萍

    2012-01-01

    为探索芦荟对微咸水灌溉栽培的适应性,研究不同盐浓度对其生长和离子吸收分配的影响.结果表明,长期(120天)以含盐溶液灌溉栽培,盐浓度达200 mmol/L NaCl显著抑制芦荟生长,100 mmol/LNaCl对芦荟生长的抑制作用显著减轻,50 mmol/L NaCl不抑制芦荟生长.同时,以50 mmol/L NaCl溶液灌溉对芦荟盐分离子吸收分配影响轻微,但盐浓度达100 mmol/LNaCl对芦荟影响显著:根、茎、叶中K+含量显著下降,Na+、Cl-含量显著增大,K+/Na+大幅减小.X-射线能谱分析结果进一步表明,叶片贮水组织是芦荟积累盐分离子的重要部位,但100 mmol/L NaCl胁迫下芦荟根尖和叶片细胞中的离子平衡受到显著干扰.结果说明,芦荟适于用微咸水灌溉栽培,叶片贮水组织在缓解其盐胁迫中可起重要作用.%To explore the adaptability of irrigating aloe with weakly brine water, the effects of different concentration salinity stresses on growth, ion absorption and distribution of aloe plants were investigated.Experimental results showed that irrigation with 200 mmol/L NaCl for 120 d significantly retarded aloe growth, but the negative effects of irrigating aloe with 100 mmol/L NaCl on aloe growth were significantly relieved, even irrigating with 50 mmol/L NaCl did not retard aloe growth.The effects of irrigating with 50 mmol/L NaCl on ion absorption, transportation and distribution of aloe plants were not significant, but irrigating with 100 mmol/L NaCl were significant: K + contents of root, stem and leaf significantly decreased, Na + and Cl- content significantly increased, K+Na+ ratio significantly decreased.X-ray microanalysis results further showed that leaf aqueous was a main part of accumulating salt ion but ion homeostasis in root and leaf cells of aloe plants were significantly interrupted under 100 mmol/L NaCl stress.The experiment results suggested that Aloe vera L.was suitable to be irrigated with weakly brine water, and leaf

  11. 硅改善盐胁迫下库拉索芦荟生长和离子吸收与分布%Silicate Improves Growth and Ion Absorption and Distribution in Aloe vera under Salt Stress

    Institute of Scientific and Technical Information of China (English)

    徐呈祥; 刘友良; 郑青松; 刘兆普

    2006-01-01

    Si 2.0 mmol/L处理明显缓解NaCl 100、200mmol/L胁迫120 d对库拉索芦荟(Aloe vera)生长的抑制作用.Si可显著降低NaCl胁迫下芦荟植株中的Na+和Cl-含量,提高K+含量,从而显著降低K+/Na+,促进根对K+的选择性吸收(ASK,Na)和K+向地上部的选择性运输(TSK,Na),以维持植株体内的离子稳态.根系和叶片横切面的X-射线能谱微区分析结果进一步证实了这一结果.Si改善盐胁迫下芦荟对K+的选择性吸收和运输的机制之一是通过显著提高盐胁迫下芦荟根细胞质膜H+-ATPase、液泡膜H+-ATPase和液泡膜H+-PPase的活性.%Si 2.0 mmol/L in irrigation solution alleviated significantly the inhibition of NaCl stress of 100 or 200mmol/L to aloe growth. Exogenously applied Si decreased significantly Na+ and Cl- contents, increased K+content and K+/Na+ ratio and selectivity ratio of absorption (ASK, Na) and of translocation (TSK, Na) to K+ and Na+in aloe plant under both NaCl 100 and 200 mmol/L stresses for 30 d. In this way, the ion homeostasis in aloe plant under NaCl stress was maintained, as was proved by X-ray microanalysis of root tip and leaf across sections.One of the mechanisms to achieve this may be the significant enhancement of H+-ATPase activities by the addition of silicate in plasma membrane and tonoplast, H+-pyrophosphatase (H+-PPase) activity in tonoplast isolated from aloe root tips under NaCl stress.

  12. Postemergence herbicides for calendula

    Science.gov (United States)

    Calendula is an alternative oilseed crop whose seed oil is valued as a substitute for tung oil and a replacement for petroleum-based volatile organic compounds in paints and other coatings. Calendula is not yet grown extensively as an agronomic crop, and its tolerances to most herbicides are unknown...

  13. 长期盐胁迫对库拉索芦荟(Aloe vera)生长和汁液理化性质的影响%EFFECT OF LONG-TERM SALT STRESS ON ALOE VERA IN GROWTH AND PHYSICAL-CHEMICAL PROPERTIES OF LEAF JUICE

    Institute of Scientific and Technical Information of China (English)

    徐呈祥; 郑青松; 刘友良; 刘兆普

    2006-01-01

    在温室中砂培条件下研究了7叶龄库拉索芦荟(Aloe vera)以含0、50、100、200和400 mmol L-1NaCl的Hoagland营养液浇灌120 d的生长响应及其全叶原汁的理化性质.结果表明:200 mmol L-1NaCl处理显著抑制芦荟生长,使叶片长度、宽度、厚度和重量显著减小,含水量和叶绿素含量降低,干枯死亡的叶片数显著增多,根系周界和根系密集区显著缩小,单株干重降至对照的65.02%,单株鲜重只及对照的38.32%,至开花期花葶低矮、细弱,开花株数明显减少;100 mmol L-1 NaCl处理对库拉索芦荟生长的抑制作用显著减轻,且可正常开花,花期未见缩短,但至处理结束时新叶的长度,处理结束时单叶的厚度和鲜重、根系密集区范围以及叶片和全株的鲜重、根系的干重和鲜重均显著小于对照,而50 mmol L-1 NaCl处理与对照无显著性差异.盐胁迫对库拉索芦荟全叶原汁出汁率及理化性质的影响与对生长开花的影响相似,其中50、100 mmol L-1 NaCl处理间多数指标值无显著性差异,与对照相比多数指标值处于有益水平.综合评判:库拉索芦荟具有咸水微咸水灌溉栽培的潜势.

  14. Nanocrystalline spinel ferrite (MFe{sub 2}O{sub 4}, M = Ni, Co, Mn, Mg, Zn) powders prepared by a simple aloe vera plant-extracted solution hydrothermal route

    Energy Technology Data Exchange (ETDEWEB)

    Phumying, Santi; Labuayai, Sarawuth; Swatsitang, Ekaphan; Amornkitbamrung, Vittaya [Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Integrated Nanotechnology Research Center (INRC), Khon Kaen University, Khon Kaen 40002 (Thailand); Maensiri, Santi, E-mail: santimaensiri@gmail.com [School of Physics, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000 (Thailand)

    2013-06-01

    Graphical abstract: This figure shows the specific magnetization curves of the as-prepared MFe{sub 2}O{sub 4} (M = Ni, Co, Mn, Mg, Zn) powders obtained from room temperature VSM measurement. These curves are typical for a soft magnetic material and indicate hysteresis ferromagnetism in the field ranges of ±500 Oe, ±1000 Oe, and ±2000 Oe for the CoFe{sub 2}O{sub 4}, MgFe{sub 2}O{sub 4} and MnFe{sub 2}O{sub 4} respectively, whereas the samples of NiFe{sub 2}O{sub 4} and ZnFe{sub 2}O{sub 4} show a superparamagnetic behavior. Highlights: ► Nanocrystalline MFe{sub 2}O{sub 4} powders were synthesized by a novel hydrothermal method. ► Metal acetylacetonates and aloe vera plant-extracted solution are used. ► This biosynthetic route is very simple and provides high-yield oxide nanomaterials. ► XRD and TEM results indicate that the prepared samples have only spinel structure. ► The maximum M{sub s} of 68.9 emu/g at 10 kOe were observed for the samples of MnFe{sub 2}O{sub 4}. - Abstract: Nanocrystalline spinel ferrite MFe{sub 2}O{sub 4} (M = Ni, Co, Mn, Mg, Zn) powders were synthesized by a novel hydrothermal method using Fe(acac){sub 3}, M(acac){sub 3} (M = Ni, Co, Mn, Mg, Zn) and aloe vera plant extracted solution. The X-ray diffraction and selected-area electron diffraction results indicate that the synthesized nanocrystalline have only spinel structure without the presence of other phase impurities. The crystal structure and morphology of the spinel ferrite powders, as revealed by TEM, show that the NiFe{sub 2}O{sub 4} and CoFe{sub 2}O{sub 4} samples contain nanoparticles, whereas the MnFe{sub 2}O{sub 4} and MgFe{sub 2}O{sub 4} samples consist of many nanoplatelets and nanoparticles. Interestingly, the ZnFe{sub 2}O{sub 4} sample contains plate-like structure of networked nanocrystalline particles. Room temperature magnetization results show a ferromagnetic behavior of the CoFe{sub 2}O{sub 4}, MnFe{sub 2}O{sub 4} and MgFe{sub 2}O{sub 4} samples, whereas the

  15. Clinical Observation of Laser Combined with Aloe Vera Pearl Capsule in the Treatment of Chloasma%像素激光联合芦荟珍珠胶囊治疗黄褐斑的临床观察

    Institute of Scientific and Technical Information of China (English)

    宋道阳; 陈义

    2013-01-01

    目的:临床观察像素激光联合芦荟珍珠胶囊对黄褐斑的疗效。方法:将符合标准的60例患者随机分为2组,治疗组和对照组各30例患者均采用以色列飞顿像素激光,波长2940nm,光斑9×9 Pixel,脉宽1ms,每4周1次;治疗组在此基础上加用芦荟珍珠胶囊。观察并拍照记录临床反应,观察周期均为12周。结果:治疗组治疗效果明显优于对照组,P<0.05,差异有统计学意义。结论:采用像素激光联合芦荟珍珠胶囊治疗黄褐斑可明显提高临床疗效。%Objective To observe the clinical curative effect of laser combined with aloe vera pearl capsule on chloasma. Methods 60 patients were divided according to the standard into 2 groups,the treatment group and the control group with 30 cases in each group were treated with Alma Lasers,with the wavelength of 2940nm,spot of 9 × 9 Pixel,pulse width 1ms and 1 time every 4 weeks. Clinical response was observed and photographed. The observation period was 12 weeks. Results The effect of the treatment group was significantly better than the control group,P < 0.05,with statistically significant difference. Conclusion Laser combined with aloe pearl capsule in the treatment of chloasma can significantly improve the clinical efficacy.

  16. 芦荟凝集素的分离、纯化和部分性质的研究%THE ISOLATION AND PURIFICATION OF LECTIN FROM ALOE VERA L. VAR. CHINENSIS (HAW.) BERGER AND ITS CHARACTERISTICS

    Institute of Scientific and Technical Information of China (English)

    高居易; 陈苒

    2000-01-01

    新鲜芦荟叶(Aloe vera L. var.chinensis(Haw.)Berger)于室温用低浓度NaCl溶液提取、离心和透析后,经N-乙酰氨基葡萄糖-Sepharose 4B亲和层析,分离纯化出芦荟凝集素(ACL).用Sephadex G-100测表观分子量为35 kD,SDS-PAGE出现两条色带:染色深的宽带和较浅的窄带.亚基分子量分别为15 kD和20kD.能专一性凝集兔血细胞和人血红细胞的A型和O型,不凝集B型和AB型.D-甘露糖和N-乙酰氨基葡萄糖能抑制凝血活性.糖蛋白内中性糖含量是4.8%.

  17. 库拉索芦荟的多倍体诱导及其变异初报%A Preliminary Study on the Polyloid Induction and Variation of Aloe vera

    Institute of Scientific and Technical Information of China (English)

    王俐; 郑思乡; 李枝林; 顾志建

    2001-01-01

    在组织培养条件下,对库拉索芦荟(Aloe vera L.)用秋水仙素进行染色体加倍的诱导处理,结果表明:用含0.06%秋水仙素处理12 h后诱变频率可达50%,其效果最佳.经秋水仙素诱导的加倍群体与正常二倍体植株比较,植株的大多数叶片变厚,叶色变深,叶片变大,气孔增大而单位叶面积气孔数减少.对变异材料进行细胞学研究后发现,体细胞中期染色体为2n=4x=28,为4倍体.未加倍前的二倍体为2n=2x=14.检测中也发现有少数植株有2n=14和2n=28两种细胞型的情况.

  18. Fast and efficient removal of silver (I) from aqueous solutions using aloe vera shell ash supported Ni0.5Zn0.5Fe2O4 magnetic nanoparticles

    Institute of Scientific and Technical Information of China (English)

    Parisa BEIGZADEH; Farid MOEINPOUR

    2016-01-01

    Silver (I) was removed from aqueous environment by aloe vera shell ash supported Ni0.5Zn0.5Fe2O4 magnetic nanoparticles. The adsorbent was characterized by several methods including X-ray diffraction (XRD), scanning electron microscopy (SEM), BET isotherm, vibrating sample magnetometer (VSM) and Fourier transform infrared spectroscopy (FT-IR). To determine the absorption of silver (I) by this adsorbent, different pH values (2−7), adsorbent dose (0.01−0.5 g), concentrations of Ag+(50, 100, 200, 300, 500, 700 and 1000 mg/L) and exposure time (5−100 min) were experimented. The highest removal efficiency of Ag+was achieved under optimum condition (30 min and pH=5). The optimum adsorbent dose was 0.20 g (in 50 mL of 100 mg/L Ag+ solution), which achieved a removal efficiency of 98.3%. The maximum monolayer adsorption capacity based on the Langmuir isotherm is 243.90 mg/g. Characterization results revealed that specific surface area and porous volume were 814.23 m2/g and 0.726 cm3/g, respectively. The experimental data were fitted well with the Langmuir and Freundlich isotherm models. Synthesized adsorbent has desired surface area and adsorptive capacity for silver (I) adsorption in aquatic environment.

  19. Final report on the safety assessment of AloeAndongensis Extract, Aloe Andongensis Leaf Juice,aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice,aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract.

    Science.gov (United States)

    2007-01-01

    Plant materials derived from the Aloe plant are used as cosmetic ingredients, including Aloe Andongensis Extract, Aloe Andongensis Leaf Juice, Aloe Arborescens Leaf Extract, Aloe Arborescens Leaf Juice, Aloe Arborescens Leaf Protoplasts, Aloe Barbadensis Flower Extract, Aloe Barbadensis Leaf, Aloe Barbadensis Leaf Extract, Aloe Barbadensis Leaf Juice, Aloe Barbadensis Leaf Polysaccharides, Aloe Barbadensis Leaf Water, Aloe Ferox Leaf Extract, Aloe Ferox Leaf Juice, and Aloe Ferox Leaf Juice Extract. These ingredients function primarily as skin-conditioning agents and are included in cosmetics only at low concentrations. The Aloe leaf consists of the pericyclic cells, found just below the plant's skin, and the inner central area of the leaf, i.e., the gel, which is used for cosmetic products. The pericyclic cells produce a bitter, yellow latex containing a number of anthraquinones, phototoxic compounds that are also gastrointestinal irritants responsible for cathartic effects. The gel contains polysaccharides, which can be acetylated, partially acetylated, or not acetylated. An industry established limit for anthraquinones in aloe-derived material for nonmedicinal use is 50 ppm or lower. Aloe-derived ingredients are used in a wide variety of cosmetic product types at concentrations of raw material that are 0.1% or less, although can be as high as 20%. The concentration of Aloe in the raw material also may vary from 100% to a low of 0.0005%. Oral administration of various anthraquinone components results in a rise in their blood concentrations, wide systemic distribution, accumulation in the liver and kidneys, and excretion in urine and feces; polysaccharide components are distributed systemically and metabolized into smaller molecules. aloe-derived material has fungicidal, antimicrobial, and antiviral activities, and has been effective in wound healing and infection treatment in animals. Aloe barbadensis (also known as Aloe vera)-derived ingredients were not toxic

  20. 库拉索芦荟和木立芦荟叶的形态结构及有效成分含量的比较%Comparative of Morphology and Effective Composition Between the Leaves of Aloe vera L. and Aloe arborescens Mill

    Institute of Scientific and Technical Information of China (English)

    李景原; 王太霞; 刘贺君; 李明泽; 刘本举; 代磊

    2008-01-01

    应用植物解剖学和植物化学相结合的方法比较研究了3年生库拉索芦荟(Aloe vera L.)和木立芦荟(Aloe arborescens Mill)的形态结构和芦荟素、芦荟多糖等有效成分的含量.研究结果表明:(1)库拉索芦荟茎极短,叶丛生,成熟叶片长60~80 cm,质量400~550 g;木立芦荟茎高50~70 cm,叶互生,成熟叶片长40~50 cm,质量100~150 g.(2)两种芦荟叶都由表皮、同化组织、贮水组织和维管束组成.库拉索芦荟叶的贮水组织占横切面的90%以上,而木立芦荟叶的贮水组织仅占横切面的70%.(3)库拉索芦荟叶中芦荟素含量为1.46%,芦荟多糖含量为3.56%,宜作为芦荟多糖的原料;木立芦荟叶中芦荟素含量为2.15%,芦荟多糖含量为2.58%,宜作为芦荟素等蒽醌类物质的原料.研究结果为芦荟的引种栽培、采收和产品加工提供了科学依据.

  1. 中华芦荟多糖的提取·精制和含量测定研究%Extraction,Purification and Content Determination of Polysaccharides in Aloe vera L. var chinensis

    Institute of Scientific and Technical Information of China (English)

    丁晨; 郭彬歆; 徐琛

    2012-01-01

    [Objective] To optimize the extraction technology of poly sac charides from Aloe vera L. var chinensis, compare several purification methods and establish a method for determining the content of polysaccharides. [ Method] With the yield and content of polysaccharides as the evaluation criteria, the extraction process of polysaccharides was optimized by orthogonal experiment. Three polysaccharides purification methods, including dialysis, macroporous resin AB-8 absorption and activated carbon absorption, were compared. The polysaccharides content was detected colorimetrically by the phenol-sulfuric acid method after the conversion coefficient of Aloe vera L. vat ckinensis polysaeeharides to glu-cose was obtained. [Result] The optimal process conditions for extraction were obtained by orthogonal experiment and the optimal parameters were as follows: the solid-liquid ratio of 1:10 g/ml, twice of extraction, and 3 h of extraction time. The contents of polysaccharide purified by the three methods were shown as dialysis > macroporous resin AB-8 > activated carbon; while the yield of polysaccharides was shown as activated carbon > dialysis > macroporous resin AB-8; the conversion coefficient of polysaccharide to glucose was determined to be 1. 32, the average recovery was 98.4% with a relative standard deviation of 2.36%. [Conclusion] The optimized extraction process is economical, simple, stable and practical, the dialysis method is simple, rapid and cheap to be used for purifying the polysaccharides. The phenol-sulfuric acid method is simple and accurate with good reproducibility.%[目的]优化中华芦荟多糖的提取工艺,比较几种精制方法,并建立多糖含量的测定方法.[方法]以多糖得率和含量为评价指标,采用正交试验法对多糖提取工艺进行研究,并比较透析法、AB-8型大孔树脂吸附法和活性炭吸附法3种多糖精制方法;采用芦荟多糖标准品测得芦荟多糖对葡萄糖的换算因子后,用苯酚-

  2. The Effect of Aloe Vera, Pomegranate Peel, Grape Seed Extract, Green Tea, and Sodium Ascorbate as Antioxidants on the Shear Bond Strength of Composite Resin to Home-bleached Enamel

    Directory of Open Access Journals (Sweden)

    Farahnaz Sharafeddin

    2015-12-01

    Full Text Available Statement of the Problem: Immediate application of bonding agent to home- bleached enamel leads to significant reduction in the shear bond strength of composite resin due to the residual oxygen. Different antioxidant agents may overcome this problem. Purpose: This study aimed to assess the effect of different antioxidants on the shear bond strength of composite resin to home-bleached. Materials and Method: Sixty extracted intact human incisors were embedded in cylindrical acrylic resin blocks (2.5×1.5 cm, with the coronal portion left out of the block. After bleaching the labial enamel surface with 15% carbamide peroxide, they were randomly divided into 6 groups (n=10. Before performing composite resin restoration by using a cylindrical Teflon mold (5×2 mm, each group was treated with one of the following antioxidants: 10% sodium ascorbate solution, 10% pomegranate peel solution, 10% grape seed extract, 5% green tea extract, and aloe vera leaf gel. One group was left untreated as the control. The shear bond strength of samples was tested under a universal testing machine (ZwickRoell Z020. The shear bond strength data were analyzed by one-way ANOVA and post hoc Tukey tests (p< 0.05. Results: No significant difference existed between the control and experimental groups. Moreover, there was no statistically significant difference between the effects of different antioxidants on the shear bond strength of bleached enamel. Conclusion: Different antioxidants used in this study had the same effect on the shear bond strength of home-bleached enamel, and none of them caused a statistically significant increase in its value.

  3. Survival in a rat model of lethal hemorrhagic shock is prolonged following resuscitation with a small volume of a solution containing a drag-reducing polymer derived from aloe vera.

    Science.gov (United States)

    Macias, Carlos A; Kameneva, Marina V; Tenhunen, Jyrki J; Puyana, Juan-Carlos; Fink, Mitchell P

    2004-08-01

    Drag-reducing polymers (DRP) increase tissue perfusion at constant driving pressure. We sought to evaluate the effects of small-volume resuscitation with a solution containing a DRP in a rat model of hemorrhage. Anesthetized rats were hemorrhaged at a constant rate over 25 min. In protocol A, total blood loss was 2.45 mL/100 g, whereas in protocol B, total blood loss was 3.15 mL/100 g. Five minutes after hemorrhage, the animals were resuscitated with 7 mL/kg of either normal saline (NS) or NS containing 50 microg/mL of an aloe vera-derived DRP. In protocol B, a third group (CON) was not resuscitated. Whole-body O2 consumption (Vo2) and CO2 production (Vco2) were measured using indirect calorimetry. In protocol A, 5/10 rats in the NS group and 8/10 rats in the DRP group survived for 4 h (P = 0.14). Mean arterial pressure was higher in the DRP-treated group than in the NS-treated group 45 min after resuscitation (89 +/- 8 vs. 68 +/- 5 mmHg, respectively; P < 0.05). In protocol B, survival rates over 2 h in the DRP, NS, and CON groups were 5/15, 1/14, and 0/7, respectively (P < 0.05). Compared with NS-treated rats, those resuscitated with DRP achieved a higher peak Vo2 (9.0 +/- 1.0 vs. 6,3+/- 1.0 mL/kg/min) and Vco2 (9.0 +/- 1.1 vs. 6.0 +/- 1.0 mL/kg/min) after resuscitation. We conclude that resuscitation with a small volume of DRP prolongs survival in rats with lethal hemorrhagic shock.

  4. Planting depth for oilseed calendula

    Science.gov (United States)

    Calendula (Calendula officinalis L.) is not only a popular ornamental plant in temperate climates, but also a potential oilseed crop. Its seed oil has high levels of calendic acid, which makes it a highly valued drying oil with important industrial applications. Much basic agronomic information on c...

  5. Induced Expression of the Gene for NADP-malic Enzyme in Leaves of Aloe vera L. under Salt Stress%盐胁迫对库拉索芦荟叶片中NADP-苹果酸酶基因的诱导表达

    Institute of Scientific and Technical Information of China (English)

    孙淑斌; 沈其荣; 万建民; 刘兆普

    2003-01-01

    A cDNA fragment for NADP-malic enzyme, catalyzing the reversible oxidative decarboxylation of L-malate to produce CO2, pyruvate and NADPH, was isolated from the leaves of a 2-month-old Aloe vera L., The level of expression of NADP-ME mRNA and accumulation of NADP-ME (AvME) protein under salt stress conditions were compared between a tolerant aloe, Aloe vera L. and a sensitive aloe, Aloe saponarea Haw. The results suggested that both the expression of the gene and the accumulation of the protein were induced in the two kinds of aloe, and the strength was related to the degree of salt tolerance. Northern blot analysis revealed that the gene for NADP-malic enzyme in Aloe vera L.(AvME) was induced by high salt, dehydration, and exogenous abscisic acid (ABA), but not by cold treatment. To further confirm whether the synthesis of AvME protein was induced with hours of treatment, Western blot analysis of the samples was conducted. The results indicated that the induction of AvME protein expression was obvious after 48 h at high salt and the level was increased with the hours of treatment.%为弄清景天酸代谢植物-库拉索芦荟中NADP-苹果酸酶(NADP-ME)基因的表达与其耐盐间的关系, 根据已知NADP-苹果酸酶序列设计引物, 从库拉索芦荟的2个月幼苗中扩增克隆了NADP-苹果酸酶496 bp的cDNA片段, 并对其进行了序列测定; 选用敏感品种皂质芦荟和耐盐品种库拉索芦荟做材料, 分别检测高盐胁迫条件下NADP-ME的表达和NADP-ME的活性. 结果表明, 两者在不同品种的芦荟中均被诱导, 但诱导的强度与芦荟的耐盐程度相关. Northern杂交分析表明, 高盐、干旱、外源ABA均能强烈诱导苹果酸酶的表达, 但寒冷对其影响不大, 这与库拉索芦荟的生物学特性相符合; 此外, 为了检测库拉索芦荟中NADP-苹果酸酶受盐诱导情况, 利用Western 印迹对样品进行了分析, 结果显示高盐条件不仅明显诱导NADP-ME的合成, 而

  6. Effect of Aloe barbadensis Mill. formulation on Letrozole induced polycystic ovarian syndrome rat model

    Directory of Open Access Journals (Sweden)

    Radha Maharjan

    2010-01-01

    Full Text Available This is a preliminary study that explores the efficacy of Aloe vera gel formulation as a possible therapeutic agent in the prevention and management of polycystic ovary syndrome (PCOS. PCOS is recognized as the most common endocrinopathy of women. Increased androgen synthesis, disrupted folliculogenesis, and insulin resistance lie at the patho-physiological core of PCOS. Current therapy for such a syndrome is use of insulin sensitizers. Large randomized clinical trials of metformin as the insulin-sensitizing drug, however, suggested that it produces many side effects after prolonged usage. For this reason, an alternate therapy would be to use herbs with hypoglycemic potential. Aloe barbadensis Mill. (Liliaceae popularly known as Aloe vera is a well-known plant with such properties. The present study evaluated the efficacy of Aloe vera gel formulation in a PCOS rat model. Five month old Charles Foster female rats were orally fed with letrozole, a non-steroidal aromatase inhibitor, to induce PCOS. The rats were then treated orally with the Aloe vera gel formulation (1 ml dose daily for 45 days. This restored their estrus cyclicity, glucose sensitivity, and steroidogenic activity. Co-treatment of the inductive agent (letrozole with the Aloe vera gel prevented the development of the PCO phenotype. Aloe vera gel formulation exerts a protective effect in against the PCOS phenotype by restoring the ovarian steroid status, and altering key steroidogenic activity. This can be attributed to phyto-components present in the extract.

  7. 珂尔维精纯芦荟胶联合异维A酸治疗轻、度痤疮疗效观察%The Therapeutic Ef ects of CareV Pure Aloe Vera Gel Combined with Isotretinoin on Treating Mild to Moderate Acne

    Institute of Scientific and Technical Information of China (English)

    周文瑞

    2014-01-01

    目的:探讨珂尔维精纯芦荟胶联合异维A酸治疗轻、中度痤疮的疗效及不良反应。方法采用随机对照分组将120入选患者分为治疗组和对照组各60例。治疗组给予珂尔维精纯芦荟胶联合异维A 酸口服治疗,对照组仅单用异维A酸口服治疗,两组均在治疗后8周判断疗效。结果治疗组有效率为89.83%,对照组为70.69%,两组比较差异有统计学意义(P<0.05),且治疗组不良反应发生率低于对照组。结论珂尔维精纯芦荟胶联合异维A酸口服治疗轻、中度痤疮具有良好的临床效果,不良反应少。%Objective To explore the therapeutic and side ef ects of CareV pure aloe vera gel combined with oral isotretinoin on treating mild to moderate acne.Methods 120 patients were randomly assigned to two groups,Each group contains 60 cases,the trail group were treated with CareV pure aloe vera gel combined with oral isotretinoin while the control group received oral isotretinoin only.Curative ef ects and side ef ects rates were evaluated in both groups after eight weeks.Results The ef ective rate of trail group was 89.83%.while it was 70.69% in control group.There was significant dif erence between the two groups.Meanwhile,the side ef ects rates were fewer in the trail group.Conclusions The therapy of CareV pure aloe vera gel combined with oral isotretinoin shows good therapeutic ef ects and few side ef ects on treating mild to moderate acne.

  8. 外源硅缓解库拉索芦荟盐胁迫伤害效应的细胞生理学分析%Cyto-physiological Analysis of Exogenous Silicon Alleviating the Damage Effect of Salt Stress on Aloe vera L.

    Institute of Scientific and Technical Information of China (English)

    徐呈祥; 马艳萍; 刘友良

    2007-01-01

    为探索硅(Si)增强微咸水长期灌溉下库拉索芦荟(Aloe vera L.)耐盐性的机制,研究了盐(NaCl 100 mmol·L-1)胁迫下加Si(2.0 mmol·L-1)处理30 d对库拉索芦荟叶同化组织细胞中矿质离子分布、叶绿体超微结构和腺苷三磷酸(ATP)酶活性的影响.X-射线能谱微域分析结果表明,盐胁迫下加Si,芦荟叶同化组织细胞中钠离子(Na+)和氯离子(Cl-)相对含量显著下降,伴随着钾离子(K+)相对含量显著升高、钾钠比(K+/Na+)显著增大.同时,加Si利于保持芦荟叶绿体正常形态,保护光合层片精细结构,显著缓解盐胁迫对其光合细胞器的伤害.利用电镜-细胞化学技术首次表明,提高浇灌溶液中可溶性Si含量水平,明显增强盐胁迫下芦荟叶同化组织细胞中胞间连丝、质膜和液泡膜ATP酶活性.外源Si增强盐胁迫下芦荟叶同化组织细胞中ATP酶活性是其改善叶同化组织细胞中的离子分布、缓解芦荟盐胁迫伤害效应的重要机制.%To explore the mechanism of added silicon (Si) enhancing salt tolerance of A. Vera L.under long-term irrigation with brackish water, the effects of exogenous Si on mineral ion distribution, chloroplast ultrastructure and adenosine triphosphatase (ATPase) activity in leaf chlorenchymatous cells of A. Vera L. NaCl-induced salt stress for 30 d were investigated. Results by energy dispersive X-ray microanalysis showed that the relative content of sodium ion (Na+) and chloride ion (Cl-) significantly decreased, coupling with a significant increase of the relative content of potassium ion (K+ ) and the K+/Na+ ratio, in leaf chlorenchymatous cells of aloe under 100 mmol·L-1 NaCl stress with added Si 2.0 mmol·L-1, so did in other three leaf tissues. Meanwhile,added Si was favourable to maintain the morphology and structure of aloe chloroplast and to protect the integrity of thylakoid structure, illustrating that exogenous soluble Si alleviated injury of salt stress to

  9. An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine.

    Science.gov (United States)

    Silva, S S; Popa, E G; Gomes, M E; Cerqueira, M; Marques, A P; Caridade, S G; Teixeira, P; Sousa, C; Mano, J F; Reis, R L

    2013-06-01

    A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe vera-based membranes. The chitosan/aloe vera-based membranes that were developed displayed satisfactory degradation, roughness, wettability and mechanical properties. A higher antibacterial potency was displayed by the blended membranes. Moreover, in vitro assays demonstrated that these blended membranes have good cell compatibility with primary human dermal fibroblasts. The chitosan/aloe vera-based membranes might be promising wound dressing materials.

  10. Studies on Some Physiological and Biochemical Indexes between Normal Plantlet and Tissue-Cultured Plantlet of Aloe vera L. var. chinensis (Haw.) Berg.%中华芦荟组培苗与正常苗某些理化特性的比较研究

    Institute of Scientific and Technical Information of China (English)

    由继红

    2001-01-01

    对中华芦荟(Aloe vera L. var. chinensis)组培苗与正常苗的干重、含水量、可溶性糖含量、叶绿素及类胡萝卜素含量、蛋白质含量及蛋白质电泳、光合速率、呼吸速率、超氧化物歧化酶活性等理化指标进行了比较研究.结果表明,中华芦荟组培苗与正常苗的上述各项指标均差异不显著.证明植物组织培养方法是中华芦荟快繁的一条有效途径,可用组培苗来代替正常的扦插苗.

  11. Meta analysis of the comparison of the effects between external application with Aloe vera and wet dressings with Magnesium sulfate in the treatment of phlebitis%芦荟外敷与硫酸镁湿敷治疗静脉炎效果比较Meta分析

    Institute of Scientific and Technical Information of China (English)

    李娜

    2011-01-01

    目的:评价芦荟外敷治疗静脉炎的效果及安全性.方法:通过检索中国期刊全文数据库(CNKI)、中文科技期刊数据库(VIP)和万方数据库截至2010年8月公开发表的、报道芦荟与常规硫酸镁湿敷治疗静脉炎的随机对照试验和临床对照试验文献,运用Cochrane系统评价方法的质量评价标准和RevMan4.2分析软件进行纳入研究的分析.结果:共纳入7 篇文献,共712例,芦荟组369例,硫酸镁组343例,合并相对危险度(RR)值及95%可信区间(CI)为1.24(1.17,1.32),总的效应检验Z=6.80(P<0.000 01).结论:芦荟外敷治疗静脉炎优于传统的硫酸镁湿敷,可作为临床证据,但今后需要更多大样本、严格设计的随机对照试验进一步验证与支持.%Objective: To evaluate the efficacy and safety of the external application with Aloe vera in the treatment of phlebitis. Methods: By researching the literatures ( published up to August 2010 ) concerning the randomized controlled trials and clinical controlled trials in the treatment of phlebitis with Aloe vera and by wet dressings with Magnesium sulfate from Chinese National Knowledge Infrastructure ( CNKI ), VIP Database for Chinese Technical Periodicals ( VIP ) and Wanfang Database, the quality evaluation criteria of Cochrane systematic review methods and RevMan 4. 2 analysis software were adopted to make the a-nalysis of included study. Results: 7 documents were included in the study ( 712 cases ), including 369 cases treated with Aloe vera and 343 cases with Magnesium sulfate. The combined relative risk ( RR ) value and 95% confidence interval ( CI) was 1. 24 ( 1. 17 , 1. 32 ); the total effects of test was Z = 6. 80 ( P <0. 000 01 ). Conclusion: The effect of external application with Aloe vera was better than that of wet dressings with Magnesium sulfate in the treatment of phlebitis, which can be used as the clinical evidence. But the results need to be further validated by a large sample and the strictly

  12. 中华芦荟两种叶型着生类屑捌湓耘喾绞教教种%The Discussion on the Planting Methods Aloe vera L.var. Chinensis (Haw)Berg and Two Kinds of Leaves

    Institute of Scientific and Technical Information of China (English)

    范眸天; 陈宗瑜; 蔡志辉; 李树云; 李云

    2000-01-01

    对中华芦荟[Aloe vera L. var. Chinensis(Haw)Bery]野生状态及引种于田间种植植株的观察中,发现从叶片着生位置看存在两种类型:(1)叶簇生(或幼时稍二列)类型;(2)叶对生类型.经多代异地无性繁殖,此性状能稳定遗传.对两类型叶片受光照射面积进行比较研究,结果表明:对生型受荫蔽面积大于簇生型,栽培条件下簇生型单株生长量大于对生型.此外,对各类型栽培方式提出了建议.

  13. EXAFS analysis of cations distribution in structure of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} nanoparticles obtained by hydrothermal method in aloe vera extract solution

    Energy Technology Data Exchange (ETDEWEB)

    Wongpratat, Unchista [Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Maensiri, Santi [School of Physics, Institute of Science, Suranaree University, Nakhonratchasima 30000 (Thailand); Swatsitang, Ekaphan, E-mail: ekaphan@kku.ac.th [Materials Science and Nanotechnology Program, Faculty of Science, Khon Kaen University, Khon Kaen 40002 (Thailand); Integrated Nanotechnology Research Center, Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand (Thailand)

    2016-09-01

    Graphical abstract: It is obvious from the M–H curves at room temperature of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles that partially substitution of the lower Bohr magneton (2 μ{sub B}) and smaller atomic radii (0.55 Å at A site, 0.69 Å at B site) of Ni{sup 2+} ions on the higher Bohr magneton (3 μ{sub B}) and larger atomic radii (0.58 Å at A site, 0.74 Å at B site) of Co{sup 2+} ions can increase the saturation magnetization (M{sub s}) of sample with x = 0.75 to approximately 1.4 times of sample with x = 0, due to the increase of the aspect ratio (surface to volume) of nanoparticles, as a result of particle size decreasing from 37.03 to 12.63 nm. In addition to this, the ferrimagnetic behavior of CoFe{sub 2}O{sub 4} has been changed to superparamagnetic behavior with the dramatic decrease of the coercivity from 1365.60 to 63.15 Oe. - Highlights: • Magnetic behavior of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} NPs depends on Ni content and size of NPs. • Distribution of Co{sup 2+} and Ni{sup 2+} ions in the structure results in the increase of M{sub s}. • Superparamagnetic behavior is observed with increasing of the aspect ratio. • M{sub s} is increased by a factor 1.4 to a value of 57.57 emu/g in Co{sub 0.25}Ni{sub 0.75}Fe{sub 2}O{sub 4}. • H{sub c} is decreased by a factor 20 to a value of 63.15 Oe in Co{sub 0.25}Ni{sub 0.75}Fe{sub 2}O{sub 4}. - Abstract: Effect of cations distribution upon EXAFS analysis on magnetic properties of Co{sub 1−x}Ni{sub x}Fe{sub 2}O{sub 4} (x = 0, 0.25, 0.50, 0.75 and 1.0) nanoparticles prepared by the hydrothermal method in aloe vera extract solution were studied. XRD analysis confirmed a pure phase of cubic spinel ferrite of all samples. Changes in lattice parameter and particle size depended on the Ni content with partial substitution and site distributions of Co{sup 2+}, Ni{sup 2+} ions of different ionic radii at both tetrahedral and octahedral sites in the

  14. [Antimicrobial activity of Calendula L. plants].

    Science.gov (United States)

    Radioza, S A; Iurchak, L D

    2007-01-01

    The sap of different organs of genus Calendula plant species has been studied for antimicrobial activity. The sap of racemes demonstrated the most expressed antimicrobial effect while that of the roots - the least one. Calendula species inhibited all tested pathogenic microorganisms, especially Pseudomonas syringae, P. fluorescens, Xanthomonas campestris, Agrobacterium tumefaciens. Calendula suffruticosa was the most active to all investigated microorganisms.

  15. Use of Random Amplified Polymorphic DNA (RAPD) Technique to Study the Genetic Diversity of Eight Aloe Species.

    Science.gov (United States)

    Ezzat, Shahira M; El Sayed, Abeer M; Salama, Maha M

    2016-10-01

    The genus Aloe comprises over 400 species of flowering succulent plants. Aloe leaves are used in the treatment of asthma, gastrointestinal ulcers, cardiovascular disease, tumors, burns, and diabetes. They are rich in anthraquinones, such as aloin, aloe-emodin, chrysophanol, aloinoside A, and aloinoside B. The various species of Aloe show chemical and morphological similarity and diversity, which depend on the genotype and environmental conditions. In a continuity to our interest in the genus Aloe, this study targets the authentication of eight different Aloe species, Aloe vera (A1), Aloe arborescens (A2), Aloe eru (A3), Aloe grandidentata (A4), Aloe perfoliata (A5), Aloe brevifolia (A6), Aloe saponaria (A7), and Aloe ferox (A8), grown in Egypt by using the technique of random amplified polymorphic DNA. Twelve decamer primers were screened in amplification with genomic DNA extracted from all species, of which five primers yielded species-specific reproducible bands. Out of 156 loci detected, the polymorphic, monomorphic, and unique loci were 107, 26, and 23, respectively. Based on a dendrogram and similarity matrix, the eight Aloe species were differentiated from each other and showed more divergence. Aloe species prevailed similarity coefficients of 54-70 % by which they could be classified into three major groups. Thus, this technique may contribute to the identification of these Aloe species that have great morphological similarity in the Egyptian local markets.

  16. Biochemical structure of Calendula officinalis.

    Science.gov (United States)

    Korakhashvili, A; Kacharava, T; Kiknavelidze, N

    2007-01-01

    Calendula officinalis is a well known medicinal herb. It is common knowledge that its medicinal properties are conditioned on biologically active complex substances of Carotin (Provitamin A), Stearin, Triterpiniod, Plavonoid, Kumarin, macro and micro compound elements. Because of constant need in raw material of Calendula officinalis, features of its ontogenetic development agro-biological qualities in various eco regions of Georgia were investigated. The data of biologically active compounds, biochemical structure and the maintenance both in flowers and in others parts of plant is presented; the pharmacological activity and importance in medicine was reviewed.

  17. 鲜芦荟外敷联合局部封闭治疗胺碘酮致静脉炎的效果观察%The observation of the clinical effect of using the fresh aloe vera which was for external application and uniting local block to treat the phlebitis which was by used amiodarone

    Institute of Scientific and Technical Information of China (English)

    龚兰娟; 叶春燕; 周小敏; 罗桂平; 关雪红; 黄丹

    2014-01-01

    Objective:It was to discuss the observation of the clinical effect of using the fresh aloe vera which was for external application and uniting local block to treat the phlebitis which was by used amiodarone with lidocaine. Method It happened to peripheral venous injection amiodarone from May 2011 to May 2013 in our hospital, which led to 50 patients had the phlebitis of grade 1 or more. And they were divided into observation and control group, each group had 25 cases. In the observation group, they used the fresh aloe vera which was for external application and someone united local block in the treatment of lidocaine with the phlebitis of grade 3 or more. In the control group, they were used by 50%magnesium sulfate wet. And it was to assess pain and contrast the clinical effect after giving drugs 4 or 8 hours. Result the clinical and analgesic effects of the observation group was better than that of the control group. Conclusion the clinical and analgesic effects of using the fresh aloe vera which was for external application and uniting local block to treat the phlebitis which was by used amiodarone with lidocaine was much better than that of using 50%magnesium sulfate wet.%目的:探讨鲜芦荟外敷联合利多卡因局部封闭治疗胺碘酮致静脉炎的临床疗效。方法:将2011年5月至2013年5月高州市人民医院收治的外周静脉输注胺碘酮致静脉炎Ⅰ级以上患者50例作为研究对象,分为观察组和对照组各25例,观察组给予鲜芦荟外敷,静脉炎Ⅲ级以上联合利多卡因局部封闭治疗,对照组给予50%硫酸镁湿敷,分别于给药后4、8 h 进行疼痛评估和对比临床疗效。结果:治疗4、8 h 后,观察组止痛效果优于对照组(P<0.05),治疗48 h 后,观察组临床疗效均优于对照组(P<0.05)。结论:采用鲜芦荟外敷联合利多卡因局部封闭治疗胺碘酮所致的静脉炎止痛效果及临床疗效明显优于50%硫酸镁湿敷。

  18. Seed germination of Calendula officinalis 'Carola' in response to temperature

    Science.gov (United States)

    Calendula (Calendula officinalis 'Carola') is a potential agronomic oilseed crop with application in the paint, coating, and cosmetic industry. Calendula has historically been used for herbal medicinal purposes and an ornamental plant. With the discovery that calendula seeds contain high concentrati...

  19. Post-emergence herbicides useful in calendula

    Science.gov (United States)

    Easy and effective weed control is required by growers who are considering new industrial crops. Post-emergence herbicides typically are the products of choice by today’s growers. Unfortunately, post-emergence herbicides with proven safety margins are not known for calendula (Calendula officinalis),...

  20. Calendula and camelina response to nitrogen fertility

    Science.gov (United States)

    The emerging oil-seed crops calendula (Calendula officinalis) and camelina (Camelina sativa L.) can provide a domestic, renewable, non-food alternative to imported oil sources for bioenergy and industrial purposes. However, very little information exists concerning nitrogen (N) fertilizer guidelines...

  1. 芦荟甘蔗复合果酒精酿工艺的实验与探究%Aloe Vera Wine Brewing Technology of Sugarcane Compound Experiment and Inquiry

    Institute of Scientific and Technical Information of China (English)

    邱怡筠; 侯周超; 高本杰; 李敬龙

    2016-01-01

    以芦荟和甘蔗为主要原料,通过实验探究采用控温发酵技术精酿芦荟、甘蔗混合果酒的工艺流程,综合实验数据确定最佳工艺:果胶酶用量为90 mg/L,SO2添加量为100 mg/L,发酵温度为22℃,糖度调整为24%,酵母接种量为8%。最终制得的甘蔗果酒感官评分91分,酒精度为12.5%vol,微生物指标合格。产品色泽微黄透亮,口感醇厚浓郁,具有甘蔗果酒独特的果香与酒香。%With aloe and sugar cane as the main raw material, through experimental research using temperature control fermentation technology, fine aloe wine, sugar cane mixed wine process, a comprehensive set of experimental data to determine optimum:pectinase dosage of 90 mg/L, addition amount of 75 mg/L SO2, fermentation temperature is 22 degrees C, sugar degree adjusted to 25%, yeast inoculation quantity is 8%. The ifnal prepared sensory score of 91 sugarcane wine, alcohol 12.5%vol, microbial indicators qualiifed. The color yellow and bright, mellow taste rich, fruity wine and sugar cane wine unique.

  2. An investigation of the potential application of chitosan/aloe-based membranes for regenerative medicine

    OpenAIRE

    SILVA, S. S.; Popa, Elena Geta; Gomes, Manuela E.; Cerqueira, M. T.; MARQUES, A.P.; Caridade, S. G.; Teixeira, P; Sousa,Cláudia; Mano, J. F.; Reis, R. L.

    2013-01-01

    A significant number of therapeutics derived from natural polymers and plants have been developed to replace or to be used in conjunction with existing dressing products. The use of the therapeutic properties of aloe vera could be very useful in the creation of active wound dressing materials. The present work was undertaken to examine issues concerning structural features, topography, enzymatic degradation behavior, antibacterial activity and cellular response of chitosan/aloe ve...

  3. The Genus Aloe: Phytochemistry and Therapeutic Uses Including Treatments for Gastrointestinal Conditions and Chronic Inflammation.

    Science.gov (United States)

    Cock, I E

    2015-01-01

    Plants of the genus Aloe have perhaps the longest recorded history of medicinal usage and are amongst the most widely used plants for traditional medicinal purposes worldwide. Aloe vera, Aloe ferox, Aloe arborescens and Aloe perryi are the best known and most widely used, but many other species are also used for their therapeutic properties. The Aloes have been used since ancient times, particularly for the treatment of microbial infections, gastrointestinal disorders and inflammatory conditions. In addition to their myriad uses in traditional therapeutics, the Aloes have also been used as components of cosmetic formulations, and in the food and beverage industries. Despite their wide acceptance, studies from different laboratories often report wide variations in the therapeutic bioactivities from within the same Aloe species, even when the same extraction procedures are used. Furthermore, leaves from individual Aloe plants within the same species may have widely varying levels of the bioactive phytochemicals. Phytochemical analyses have shown that many Aloe species contain various carbohydrate polymers (notably glucomannans) and a range of other low molecular weight phenolic compounds including alkaloids, anthraquinones, anthrones, benzene and furan derivatives, chromones, coumarins, flavonoids, phytosterols, pyrans and pyrones. There has been a wealth of information published about the phytochemistry and therapeutic potential of the Aloes (especially Aloe vera). Much of this has been contradictory. Intra- and interspecies differences in the redox state of the individual Aloe components and in the ratios of these components may occur between individual plants. These factors may all affect the physiological properties of Aloe extracts. Due to the structure and chemical nature of many of the Aloe phytochemicals, it is likely that many of the reported medicinal properties are due to antioxidant or prooxidant effects. The antioxidant/prooxidant activities of many Aloe

  4. Growth and yield response of calendula (Calendula officinalis) to sowing date in the northern U.S.

    Science.gov (United States)

    Calendula (Calendula officinalis L.) seed is a rich source of the conjugated C18:3 fatty acid calendic acid and can serve as a replacement for VOCs in many industrial chemicals such as paints, coatings and adhesives. Calendula is widely adapted to temperate climates and may be a beneficial rotationa...

  5. Effect of Aaloe vera Gel Versus Local triamcinolone in Treatment of Oral Lichen Planus

    Directory of Open Access Journals (Sweden)

    Amanat D.

    2011-02-01

    Full Text Available Statement of Problem: Oral lichen planus (OLP is a chronic inflammatory disorder which can specially be painful in atrophic, erosive and ulcerative forms. In this area, different treatment plans with different results have been evaluated Purpose: The aim of this article is to compare the effect of aloe vera gel and local triamcinolone in treatment of oral lichen planus lesions.Materials and Method: In this double blind research, 50 patients (13 males and 37 females having oral lichen planus lesions were selected and randomly divided into two groups. The first group received 70% aloe vera gel and the second group, 0.1% local triamcinolone. Both groups used the drug locally 3 times a day for 8 weeks. All the patients were under follow up every 2 weeks. Clinical evaluation and pain reduction was evaluated by Thongprasom and VAS methods in both groups. Data were evaluated by repeated measure technique.Results: 20 patients of the triamcinolone group showed good response to treatment and 3 patients had complete remission during the treatment period. In aloe vera group, 9 patients had good response and no one showed complete remission ( p <0.001. No significant lesion was seen in both groups during the treatment period Conclusion: Application of aloe vera gel is suggested in treatment of erosive and atrophic lichen planus lesions in comparison with steroids. However, according to the results of this study, the use of steroid instead of aloe vera in treating ulcerative lichen planus lesions is a better and more appropriate choice.

  6. Variation in seed lipids in Calendula germplasm

    Science.gov (United States)

    Calendula officinalis (pot marigold) has considerable promise as an industrial crop, with a long history as an ornamental and medicinal plant. It is also marketed as an ingredient in cosmetics and a colorant. It produces unusual seed lipids, which can provide an additional market for commercial Ca...

  7. 2种芦荟中芦荟苷含量及稳定性研究%Research on the Content and Stability of Aloin in Two Kinds of Aloes

    Institute of Scientific and Technical Information of China (English)

    吴洪; 蔡和意

    2012-01-01

    [目的]对不同生长期的中华芦荟(Aloe vera var.chinensis)和库拉索芦荟(Aloe vera)中芦荟苷含量进行测定,并测定在紫外光和不同酸碱条件下芦荟的稳定性.[方法]采用等吸收双波长分光光度法测定芦荟中芦荟苷含量.[结果]4年生中华芦荟和2年生库拉索芦荟中芦荟苷含量最高,且芦荟苷主要存在于叶皮中;中华芦荟的稳定性随生长期增加而增强,且在弱酸性条件下稳定性较好.[结论]该研究可为合理地采收、开发利用芦荟资源及在储存过程中保留更多的活性成分提供理论指导.%[ Objective ] To determine the content of aloin in Aloe vera L. var. chinesis and Aloe barbadensis Mill at different growth periods, as well as the stability of aloes under the ultraviolet light and different pH conditions. [ Method] The content of aloin in aloes was determined by dual wavelength spectrophotometry. [ Result] The aloin was the highest in the four-year-old Aloe vera L. var. chinesis and the two-year-old Aloe barbadensis Mill, and it mainly existed in the leaf cortex; the stability of Aloe vera L. var. chinesis enhanced with Lhe prolonged growth period , and reached the best under weak acidic condition. [ Conclusion] The study provides theoretical guidance for the reasonable harvesting and utilization of aloe and the preservation of more active components during storage.

  8. Aloe chemical constituents and pharmacological research%芦荟的化学成分及药理作用

    Institute of Scientific and Technical Information of China (English)

    翟莲; 宋凯凯

    2014-01-01

    芦荟作为一种常见的药用植物,已越来越受到人们的关注。其为百合科植物库拉索芦荟Aloe barbadensisMil er、好望角芦荟Aloe feroxMil er或其他同属近缘植物叶的汁液浓缩干燥物,含有蒽醌类、糖类、维生素类、有机酸类等多种有效成分,具有通便泻下、抗肿瘤、抗糖尿病等多种的药理作用。%Aloe vera as a common medicinal plants, has been more and more attention. The lily family plant Aloe vera Aloe barbadensisMil er, Cape Aloe Aloe feroxMil er same relatives or other juice concentrate dried leaf material, containing anthraquinone, carbohydrate, vitamins, organic acids and other active ingredients with constipation purgative, anti-tumor, anti-diabetic and other pharmacological effects.

  9. Antifungal effects of different plant extracts and their major components of selected aloe species.

    Science.gov (United States)

    Ali, M I; Shalaby, N M; Elgamal, M H; Mousa, A S

    1999-08-01

    Different extracts of both fresh and dry leaves of Aloe eru A. Berger, A. vera L. Webb & Berth and A. arborescens Mill. were screened for their antifungal activity against Aspergillus niger, Cladosporium herbarum and Fusarium moniliforme. The toxicity of the isolated pure components were evaluated on the tested fungi. A comparative chromatographic study was performed to differentiate between natural components existing in various fractions and extracts of Aloe species and specific spray reagents were used for the detection of anthraquinones in the isolated components.

  10. ALOS-2 initial results

    Science.gov (United States)

    Kankaku, Yukihiro; Suzuki, Shinichi; Shimada, Masanobu

    2015-10-01

    The Advanced Land Observing Satellite-2 (ALOS-2) was launched from Tanegashima Space Center by H-IIA rocket successfully on 24th May 2014. ALOS-2 carries the Phased Array type L-band Synthetic Aperture Radar-2 (PALSAR-2) as the state-of-the-art L-band SAR system which succeeds to PALSAR onboard ALOS. PALSAR-2 uses almost whole bandwidth allocated for L-band active sensor of Earth Exploration Satellites Service specified by the Radio Regulation in order to realize the high resolution observation, and also, it transmits more than 6 kW power for lower Noise Equivalent Sigma Zero using 180 TRMs driven by Gallium Nitride (GaN) amplifier which is the first use in space. Furthermore, because ALOS-2 carries the SAR system only, PALSAR-2 antenna can be mounted under the satellite body. It enables to observe right-/left-looking observation by satellite maneuvering. And the high accuracy orbit control to maintain the satellite within 500 m radius tube against the reference orbit enables high coherence for the InSAR processing. Using these new technologies, ALOS-2 has been operating to fulfill the mission requirements such as disaster monitoring and so on. This document introduces the initial result of ALOS-2 from the first year operation.

  11. Aloe Gel Enhances Angiogenesis in Healing of Diabetic Wound

    Directory of Open Access Journals (Sweden)

    Djanggan Sargowo

    2011-12-01

    Full Text Available BACKGROUND: Diabetic micro and macroangiophathy lead to the incident of diabetic foot ulcers characterized by an increased number of circulating endothelial cells (CECs and decreased function of endothelial progenitor cells (EPCs. This fact is correlated with ischemia and diabetic wound healing failure. Aloe vera gel is known to be able to stimulate vascular endothelial growth factor (VEGF expression and activity by enhancing nitric oxide (NO production as a result of nitric oxide synthase (NOS enzyme activity. Aloe vera is a potential target to enhancing angiogenesis in wound healing. OBJECTIVE: The objective of this study was to explore the major role of Aloe vera gel in wound healing of diabetic ulcers by increasing the level of EPCs, VEGF, and endothelial nitric oxide synthase (eNOS, as well as by reducing the level of CECs involved in angiogenesis process of diabetic ulcers healing. METHODS: The experimental groups was divided into five subgroups consisting of non diabetic wistar rats, diabetic rats without oral administration of aloe gel, and treatment subgroup (diabetic rats with 30, 60 and 120 mg/day of aloe gel doses for 14 days. All subgroups were wounded and daily observation was done on the wounds areas. Measurement of the number of EPCs (CD34, and CECs (CD45 and CD146 was done by flow cytometry, followed by measurement of VEGF and eNOS expression on dermal tissue by immunohistochemical method on day 0 and day 14 after treatment. The quantitative data were analyzed by One-Way ANOVA and Linear Regression, with a confidence interval 5% and significance level (p<0.05 using SPSS 16 software to compare the difference and correlation between wound diameters, number of EPCs and CECs as well as the levels of VEGF and eNOS. RESULTS: The results of this study showed that aloe gel oral treatment in diabetic wistar rats was able to accelerate the wound healing process. It was shown by significant reduction of wound diameter (0.27±0.02; the

  12. VERA 3.5 Installation Guide

    Energy Technology Data Exchange (ETDEWEB)

    Bartlett, Roscoe A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Baird, Mark L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Berrill, Mark A. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Kulesza, Joel A. [Univ. of Michigan, Ann Arbor, MI (United States); Mervin, Brenden T. [Electric Power Research Inst. (EPRI), Charlotte, NC (United States)

    2016-08-16

    This guide describes the structure and setup of the standard VERA development environment (VERA Dev Env) and standard VERA Third Party Libraries (TPLs) that need to be in place before installing many of the VERA simulation components. It describes everything from the initial setup on a new machine to the final build, testing, and installation of VERA components. The goal of this document is to describe how to create the directories and contents outlined in Standard VERA Dev Env Directory Structure and then obtain the remaining VERA source and build, test, and install any of the necessary VERA components on a given system. This document describes the process both for a development version of VERA and for a released tarball of the VERA sources.

  13. A review on phytochemistry and ethnopharmacological aspects of genus Calendula.

    Science.gov (United States)

    Arora, Disha; Rani, Anita; Sharma, Anupam

    2013-07-01

    This review includes 84 references on the genus Calendula (Asteraceae) and comprises ethnopharmacology, morphology and microscopy, phytoconstituents, pharmacological reports, clinical studies and toxicology of the prominent species of Calendula. Triterpene alcohols, triterpene saponins, flavonoids, carotenoids and polysaccharides constitute major classes of phytoconstituents of the genus. A few species of this genus have medicinal value, among these Calendula officinalis Linn., has been traditionally used in the treatment of various skin tumors, dermatological lesions, ulcers, swellings and nervous disorders as well as almost 200 cosmetic formulations, i.e., creams, lotions, shampoos. Despite a long tradition of use of some species, the genus has not been explored properly. In the concluding part, the future scope of Calendula species has been emphasized with a view to establish their multifarious biological activities and mode of action.

  14. A novel method for air drying aloe leaf slices by covering with filter papers as a shrink-proof layer.

    Science.gov (United States)

    Kim, S A; Baek, J H; Lee, S J; Choi, S Y; Hur, W; Lee, S Y

    2009-01-01

    To prevent the shrinkage of aloe vera slices during air drying, a method utilizing a shrink-proof layer was developed. The sample was configured of whole leaf aloe slices, where 1 side or both sides were covered with filter papers as shrink-proof layers. After air drying by varying the air temperature and the slice thickness, the drying characteristics, as well as several quality factors of the dried aloe vera leaf slices, were analyzed. In the simulation of the drying curves, the modified Page model showed the best fitness, representing a diffusion-controlled drying mechanism. Nonetheless, there was a trace of a constant-rate drying period in the samples dried by the method. Shrinkage was greatly reduced, and the rehydration ratios increased by approximately 50%. Scanning electron microscopic analysis revealed that the surface structure of original fibrous form was well sustained. FT-IR characteristics showed that the dried samples could sustain aloe polysaccharide acetylation. Furthermore, the functional properties of the dried slices including water holding capacity, swelling, and fat absorption capability were improved, and polysaccharide retention levels increased by 20% to 30%. Therefore, we concluded that application of shrink-proof layers on aloe slices provides a novel way to overcome the shrinkage problems commonly found in air drying, thereby improving their functional properties with less cost. Practical Application: This research article demonstrates a novel air drying method using shrink-proof layers to prevent the shrinkage of aloe slices. We analyzed extensively the characteristics of shrinkage mechanism and physical properties of aloe flesh gels in this drying system. We concluded that this method can be a beneficial means to retain the functional properties of dried aloe, and a potential alternative to freeze drying, which is still costly.

  15. Analysis of carotenoid composition in petals of calendula (Calendula officinalis L.).

    Science.gov (United States)

    Kishimoto, Sanae; Maoka, Takashi; Sumitomo, Katsuhiko; Ohmiya, Akemi

    2005-11-01

    Nineteen carotenoids were identified in extracts of petals of orange- and yellow-flowered cultivars of calendula (Calendula officinalis L.). Ten carotenoids were unique to orange-flowered cultivars. The UV-vis absorption maxima of these ten carotenoids were at longer wavelengths than that of flavoxanthin, the main carotenoid of calendula petals, and it is clear that these carotenoids are responsible for the orange color of the petals. Six carotenoids had a cis structure at C-5 (C-5'), and it is conceivable that these (5Z)-carotenoids are enzymatically isomerized at C-5 in a pathway that diverges from the main carotenoid biosynthesis pathway. Among them, (5Z,9Z)-lycopene (1), (5Z,9Z,5'Z,9'Z)-lycopene (3), (5'Z)-gamma-carotene (4), and (5'Z,9'Z)-rubixanthin (5) has never before been identified. Additionally, (5Z,9Z,5'Z)-lycopene (2) has been reported only as a synthesized compound.

  16. Effect of Semiarid Environment on Some Nutritional and Antinutritional Attributes of Calendula (Calendula officinalis

    Directory of Open Access Journals (Sweden)

    Muhammad Iqbal

    2015-01-01

    Full Text Available Stressful environments have been shown to affect the metabolism in some plants. In the present study, we assessed whether semiarid environment (saline and saline alkaline soil could affect the nutritional (total proteins, phenolics and riboflavin contents, and catalase activity and antinutritional (hydrogen peroxide and malondialdehyde contents properties differently in different plant parts (leaves, flowers, and roots of calendula. Although salinity decreased plant biomass, it did not affect total protein and phenolics contents in the calendula. All plant parts were rich in riboflavin contents. However, plants grown under saline-alkali soil had relatively more riboflavin contents in the flowers. Salinity increased hydrogen peroxide (H2O2 concentration in the flowers and roots, whereas saline-alkali soil did not affect it. Plants exposed to both saline and saline-alkali soil had greater catalase activity in the flowers and leaves. Plants exposed to salinity had higher malondialdehyde (MDA contents in the flowers compared with nonsaline and saline-alkali conditions. Nonetheless, the possibility of safely using different parts of calendula as nutraceutical was in the order flower > root > leaf. Overall, the results suggested that plant can be grown in mild saline-alkali (EC ≤ 7; pH = 8.5 soils without affecting its nutraceutical properties.

  17. The performance of young pigs fed different amounts of marigold (Calendula officinalis) meal; a pilot study

    NARCIS (Netherlands)

    Hindle, V.A.; Mathijssen-Kamman, A.A.; Stockhofe, N.; Cone, J.W.

    2002-01-01

    Hexane-extracted calendula meal was tested in an acceptance trial with pigs to determine their response to calendula meal. Performance parameters included feed intake, daily growth and post mortem histopathological examination of vital organs. Although calendula meal showed potential as a ration ing

  18. Calendula: A Potential New Oilseed Crop for the Northern U.S.

    Science.gov (United States)

    Seeds of calendula (Calendula officinalis) are a rich source of calendic acid (conjugated C18:3), a highly oxidative fatty acid that can be used to replace volatile organic compounds (VOC) as a drying agent in many industrial chemicals including paints and adhesives. Calendula flourishes in temperat...

  19. Assessment of In vitro Sun Protection Factor of Calendula Officinalis L. (Asteraceae) Essential Oil Formulation.

    Science.gov (United States)

    Mishra, Ak; Mishra, A; Chattopadhyay, P

    2012-01-01

    The present study was undertaken to study the sunscreen activity of herbal formulation. There is no evidence of the sun protection factor (SPF) studies on essential oil of Calendula flowers (Calendula officinalis L., Asteraceae). The study investigates the in vitro SPF by ultraviolet specrtophotometry method of Calendula flower oil in a cream formulation. Calendula oil was isolated by Clavenger's apparatus, compositions were identified by GC-MS and the cream of calendula flower oil was prepared by homogenization method followed by evaluation for physical parameters. The sun protection factor of cream was evaluated by in vitro method employing UV-visible spectrophotometer (Shimazdu-1600). The SPF of Calendula oil in cream formulation exhibited good activity (SPF = 14.84 ± 0.16). Finding of this study suggested that calendula oil cream can be used to protect the skin from UV radiations in form of sunscreen cream and to maintain the natural pigmentation of the skin.

  20. Amelioration of myocardial ischemic reperfusion injury with Calendula officinalis.

    Science.gov (United States)

    Ray, Diptarka; Mukherjee, Subhendu; Falchi, Mario; Bertelli, Aldo; Das, Dipak K

    2010-12-01

    Calendula officinalis of family Asteraceae, also known as marigold, has been widely used from time immemorial in Indian and Arabic cultures as an anti-inflammatory agent to treat minor skin wound and infections, burns, bee stings, sunburn and cancer. At a relatively high dose, calendula can lower blood pressure and cholesterol. Since inflammatory responses are behind many cardiac diseases, we sought to evaluate if calendula could be cardioprotective against ischemic heart disease Two groups of hearts were used: the treated rat hearts were perfused with calendula solution at 50 mM in KHB buffer (in mM: sodium chloride 118, potassium chloride 4.7, calcium chloride 1.7, sodium bicarbonate 25, potassium biphosphate 0.36, magnesium sulfate 1.2, and glucose 10) for 15 min prior to subjecting the heart to ischemia, while the control group was perfused with the buffer only. Calendula achieved cardioprotection by stimulating left ventricular developed pressure and aortic flow as well as by reducing myocardial infarct size and cardiomyocyte apoptosis. Cardioprotection appears to be achieved by changing ischemia reperfusion-mediated death signal into a survival signal by modulating antioxidant and anti-inflammatory pathways as evidenced by the activation of Akt and Bcl2 and depression of TNFα. The results further strengthen the concept of using natural products in degeneration diseases like ischemic heart disease.

  1. VERA and VERA-EDU 3.5 Release Notes

    Energy Technology Data Exchange (ETDEWEB)

    Sieger, Matt [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Salko, Robert K. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Kochunas, Brendan M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Adams, Brian M. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Williamson, Richard L. [Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

    2016-07-26

    The Virtual Environment for Reactor Applications components included in this distribution include selected computational tools and supporting infrastructure that solve neutronics, thermal-hydraulics, fuel performance, and coupled neutronics-thermal hydraulics problems. The infrastructure components provide a simplified common user input capability and provide for the physics integration with data transfer and coupled-physics iterative solution algorithms. Neutronics analysis can be performed for 2D lattices, 2D core and 3D core problems for pressurized water reactor geometries that can be used to calculate criticality and fission rate distributions by pin for input fuel compositions. MPACT uses the Method of Characteristics transport approach for 2D problems. For 3D problems, MPACT uses the 2D/1D method which uses 2D MOC in a radial plane and diffusion or SPn in the axial direction. MPACT includes integrated cross section capabilities that provide problem-specific cross sections generated using the subgroup methodology. The code can be executed both 2D and 3D problems in parallel to reduce overall run time. A thermal-hydraulics capability is provided with CTF (an updated version of COBRA-TF) that allows thermal-hydraulics analyses for single and multiple assemblies using the simplified VERA common input. This distribution also includes coupled neutronics/thermal-hydraulics capabilities to allow calculations using MPACT coupled with CTF. The VERA fuel rod performance component BISON calculates, on a 2D or 3D basis, fuel rod temperature, fuel rod internal pressure, free gas volume, clad integrity and fuel rod waterside diameter. These capabilities allow simulation of power cycling, fuel conditioning and deconditioning, high burnup performance, power uprate scoping studies, and accident performance. Input/Output capabilities include the VERA Common Input (VERAIn) script which converts the ASCII common input file to the intermediate XML used to drive all of the physics

  2. “IN VITRO” MULTIPLICATION OF CALENDULA OFFICINALIS L.

    Directory of Open Access Journals (Sweden)

    Vantu Smaranda

    2015-11-01

    Full Text Available The aim of the present study was to develop a regeneration procedures for Calendula officinalis L., as analternative for biomass production. Calendula officinalis L. (Asteraceae is an important medicinal plant species withmultitherapeutic, cosmetic, values. Meristematic explants taken from seedlings of Calendula officinalis L. germinated inaseptic conditions were tested for their regenerative potential. The regeneration of whole plants was obtained in twosteps: the shoots were excised and transferred to fresh medium and then rooting of these shoots was achieved on the samemedium with 0,02 mg/l benzylaminopurine and 1 mg/l 2,4 dichlorophenoxyacetic acid. The excised shoots weresubcultured for roots induction. Regenerated plants were transferred to ex vitro conditions for an acclimatisation period

  3. Whatever Happened to Vera?

    Directory of Open Access Journals (Sweden)

    Jo Henderson

    2013-12-01

    Full Text Available The road to technological progress is littered with unsuccessful prototypes and their inventors, and in British television there is perhaps no better example than John Logie Baird, universally recognised as the inventor of the technology, but not the successful business model. Another casualty is the Vision Electronic Recording Apparatus (VERA, less well known than Baird’s invention but a technology developed by the BBC Design Unit that has the potential to change the production and working practices of British television, in ways yet to be imagined or apparent. This article, based on secondary sources (Briggs: 1995, Burns:1977,Hall: 1996, Hartney: 1996 Marshall: 1979 Nash: 1970, and Wyver:1981, 1989, seeks to illuminate and narrativise some of the threads in the hidden, or certainly largely unexplored, history of video technology in British television between 1955 and 1975. The start date recognises the ending the BBC’s television monopoly and the shift to a duopolistic industry that the BBC has to adjust to. The end date reflects a point where non-broadcast video technology has become more affordable and of such high quality that it threatens to achieve the standard previously set as the minimum by the broadcasting unions. The affordability of the technology leads to new forms of content and new contexts of practice developing amongst artists, activists and auteurs, some of whom explicitly choose to question the constructs of television.

  4. EFFECTS OF P APPLICATION ON N AND P ABSORPTION AND SUGAR ACCUMULATION BY 1-YEAR-OLD ALOE VERA IRRIGATED WITH SEAWATER%施磷对海水灌溉下一年生芦荟氮磷吸收及糖分积累的影响

    Institute of Scientific and Technical Information of China (English)

    邵晶; 刘玲; 刘兆普; 宁建凤; 郑青松

    2008-01-01

    在对大田海水灌溉试验的基础上,进行海水灌溉下的肥料试验。库拉索芦荟(Aloe vera)是一种CAM光合碳同化途径的多年生旱生经济植物,具有一定的耐盐性。在国外,芦荟研究的成果被广泛应用在医药、保健、食品等行业,已形成一大产业。最近的研究报告表明,与其他植物一样,盐分胁迫造成芦荟体内盐分积累、营养元素缺失,

  5. 芦荟提取物对常见食品污染菌的抑制作用研究%The Study on Inhibition of AloeExtract to Common Food Contamination Microbiology

    Institute of Scientific and Technical Information of China (English)

    申晓慧

    2015-01-01

    为了验证芦荟提取物的抑菌效果,采用滤纸片法、固体稀释法观察芦荟汁对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌和黑曲霉的抑菌效果。结果表明:芦荟汁对供试菌生长有明显的抑制作用,但是滤纸片测定法抑菌效果不明显,固体稀释法较好,100%的芦荟汁的抑菌效果较好,50%和70%的芦荟汁抑菌效果较差。%To verify the bacteriostatic effect of Aloe vera extract, the filter paper method and solid dilution method were used to observe the bacteriostatic effect of the aloe juice on Bacillus subtilis, Escherichia coli, Staphylococcusaureusand Aspergillusniger. The results showed that:the Aloe vera juice inhibited the selected bacteria growth obviously, but the antibacterial effect of filter paper was not obvious, the solid dilution method was better, the bacteriostatic effect of 100%Aloe vera juice was good, the bacteriostatic effect of 50%and 70%aloe juice were poor.

  6. Antibacterial Effects of Extract of Alloe Vera and Stachys Inflata on Gram Positive and Negative Bacteria in In Vitro

    Directory of Open Access Journals (Sweden)

    Rajabi, M. (MSc

    2014-09-01

    Full Text Available Background and objective: Antimicrobial and antiviral effects of Alloe Vera and Stachys inflata have been proved. We aimed to investigate the effects of extract of Alloe Vera and Stachys inflate on the growth of some bacteria to take the place of chemical drugs. Material and Methods: the extracts of both plants were prepared by maceration method; different concentrations were prepared using Mueller Hinton agar medium and tested by Disc diffusion. Furthermore, minimum inhibitory concentration (MIC and minimum bactericidal concentration (MBC were determined by the Microdilution method. Results: The effect of Alloe Vera extract was significant on Staphylococcus aureus. MIC and MBC of Aloe Vera extract on Bacillus subtilis were obtained in 230 and 410 mg/ml, respectively, which were 500 and 714 mg/ml for Haemophilus influenza. The extract of gel of Alloe Vera had no effect on Bacillus subtilis. The extract of leaf and gel of Alloe Vera had an inhibitory effect on Haemophilus influenza and Pseudomonads aeraginosa. The extract of Stachys inflata had an inhibitory effect on Haemophilus influenza, but it did not have any on Pseudomonads aeraginosa. The Extract of Stachys inflata had no effect on Bacillus subtilis, while showing significant effect on Staphylococcus. Among antibiotics, Ofloxacin had an effect on Haemophilus influenza. The extract of both plants did not show any effect on Klebsiella pneumonia. Conclusion: Given the effect of Alloe Vera and Stachys inflata in laboratory conditions, we hope that these extracts will be used instead of chemical substances for making nutritional supplements to control human diseases.

  7. Solid lipid nanoparticles for delivery of Calendula officinalis extract.

    Science.gov (United States)

    Arana, Lide; Salado, Clarisa; Vega, Sandra; Aizpurua-Olaizola, Oier; de la Arada, Igor; Suarez, Tatiana; Usobiaga, Aresatz; Arrondo, José Luis R; Alonso, Alicia; Goñi, Félix M; Alkorta, Itziar

    2015-11-01

    Solid lipid nanoparticles (SLN) composed of long-chain fatty acids (palmitic acid, stearic acid or arachidic acid), Epikuron 200 (purified phosphatidylcholine), and bile salts (cholate, taurocholate or taurodeoxycholate) have been prepared by dilution of a microemulsion. A total of five different systems were prepared, and characterized by photon correlation spectroscopy, transmission electron microscopy, differential scanning calorimetry, and infrared spectroscopy. The SLN formulation showing optimal properties (lowest size and polydispersity index and highest zeta potential) was obtained with stearic acid and taurodeoxycholate as cosurfactant. This formulation was loaded with Calendula officinalis extract, a natural compound used on ophthalmic formulations given its anti-inflammatory, emollient, and wound repairing activity. Calendula-loaded SLN preparations were characterized in order to determine loading capacity and entrapment efficiency. In vitro cytotoxicity and wound healing efficacy of Calendula-loaded SLN compared to that of a free plant extract were evaluated on a conjunctival epithelium cell line WKD. Our results suggest that this SLN formulation is a safe and solvent-free Calendula extract delivery system which could provide a controlled therapeutic alternative for reducing disease-related symptoms and improving epithelium repair in ocular surface.

  8. Determination of the polysaccharides contents in three aloe of Fujian province%闽产三种芦荟叶汁中多糖含量测定

    Institute of Scientific and Technical Information of China (English)

    包国荣; 陈建斌; 陈玉枝

    2001-01-01

    本文采用硫酸-酚比色法,于490nm波长测定斑芦荟(AIoe vera L.Var.chinensis (Haw)Berg)、库拉索芦荟(Aloe barbadensis miller)、以及木立芦荟(Aloe arborescens mill)叶汁中的多糖含量,结果表明:库拉索芦荟中的多糖含量较高,斑纹芦荟次之,木立芦荟最低.

  9. 芦荟罐头生产工艺%Main Process of Aloe Can

    Institute of Scientific and Technical Information of China (English)

    赵华

    2005-01-01

    芦荟是一种百合科草本植物,民间自古就有使用芦荟的记载。芦荟及其变种各类很多,目前在我国种植和加工较多的库拉索芦荟(Aloe Vera L.)。木立芦荟(A. arborescens Mill)和华芦荟(A.Varavia),还有少量的皂质芦荟和开谱敦芦荟等。

  10. Precise Orbit Determination for ALOS

    Science.gov (United States)

    Nakamura, Ryo; Nakamura, Shinichi; Kudo, Nobuo; Katagiri, Seiji

    2007-01-01

    The Advanced Land Observing Satellite (ALOS) has been developed to contribute to the fields of mapping, precise regional land coverage observation, disaster monitoring, and resource surveying. Because the mounted sensors need high geometrical accuracy, precise orbit determination for ALOS is essential for satisfying the mission objectives. So ALOS mounts a GPS receiver and a Laser Reflector (LR) for Satellite Laser Ranging (SLR). This paper deals with the precise orbit determination experiments for ALOS using Global and High Accuracy Trajectory determination System (GUTS) and the evaluation of the orbit determination accuracy by SLR data. The results show that, even though the GPS receiver loses lock of GPS signals more frequently than expected, GPS-based orbit is consistent with SLR-based orbit. And considering the 1 sigma error, orbit determination accuracy of a few decimeters (peak-to-peak) was achieved.

  11. Phenotypic and biochemical profile changes in calendula (Calendula officinalis L.) plants treated with two chemical mutagenesis.

    Science.gov (United States)

    El-Nashar, Y I; Asrar, A A

    2016-05-06

    Chemical mutagenesis is an efficient tool used in mutation-breeding programs to improve the vital characters of the floricultural crops. This study aimed to estimate the effects of different concentrations of two chemical mutagens; sodium azide (SA) and diethyl sulfate (DES). The vegetative growth and flowering characteristics in two generations (M1 and M2) of calendula plants were investigated. Seeds were treated with five different concentrations of SA and DES (at the same rates) of 1000, 2000, 3000, 4000, and 5000 ppm, in addition to a control treatment of 0 ppm. Results showed that lower concentrations of SA mutagen had significant effects on seed germination percentage, plant height, leaf area, plant fresh weight, flowering date, inflorescence diameter, and gas-exchange measurements in plants of both generations. Calendula plants tended to flower earlier under low mutagen concentrations (1000 ppm), whereas higher concentrations delayed flowering significantly. Positive results on seed germination, plant height, number of branches, plant fresh weight, and leaf area were observed in the M2-generation at lower concentrations of SA (1000 ppm), as well as at 4000 ppm DES on number of leaves and inflorescences. The highest total soluble protein was detected at the concentrations of 1000 ppm SA and 2000 ppm DES. DES showed higher average of acid phosphatase activity than SA. Results indicated that lower concentrations of SA and DES mutagens had positive effects on seed germination percentage, plant height, leaf area, plant fresh weight, flowering date, inflorescence diameter, and gas-exchange measurements. Thus, lower mutagen concentrations could be recommended for better floral and physio-chemical performance.

  12. Expression of hsp70, hsp100 and ubiquitin in Aloe barbadensis Miller under direct heat stress and under temperature acclimation conditions.

    Science.gov (United States)

    Huerta, Claudia; Freire, Matías; Cardemil, Liliana

    2013-02-01

    KEY MESSAGE : The study determined the tolerance of Aloe vera to high temperature, focusing on the expression of hsp70 , hsp100 and ubiquitin genes. These were highly expressed in plants acclimated at 35 °C prior to a heat shock of 45 °C. Aloe barbadensis Miller (Aloe vera), a CAM plant, was introduced into Chile in the semiarid IV and III Regions, which has summer diurnal temperature fluctuations of 25 to 40 °C and annual precipitation of 40 mm (dry years) to 170 mm (rainy years). The aim of this study was to investigate how Aloe vera responds to water and heat stress, focusing on the expression of heat shock genes (hsp70, hsp100) and ubiquitin, which not studied before in Aloe vera. The LT(50) of Aloe vera was determined as 53.2 °C. To study gene expression by semi-quantitative RT-PCR, primers were designed against conserved regions of these genes. Sequencing the cDNA fragments for hsp70 and ubiquitin showed a high identity, over 95 %, with the genes from cereals. The protein sequence of hsp70 deduced from the sequence of the cDNA encloses partial domains for binding ATP and the substrate. The protein sequence of ubiquitin deduced from the cDNA encloses a domain for interaction with the enzymes E2, UCH and CUE. The expression increased with temperature and water deficit. Hsp70 expression at 40-45 °C increased 50 % over the controls, while the expression increased by 150 % over the controls under a water deficit of 50 % FC. The expression of all three genes was also studied under 2 h of acclimation at 35 or 40 °C prior to a heat shock at 45 °C. Under these conditions, the plants showed greater expression of all genes than when they were subjected to direct heat stress.

  13. Investigation of Morphological and Mechanical Properties of PVA/ Aloe barbadensis and PEO/Chitosan Blended Nanofibrous Structures Fabricated by Dual Electrospinning System

    OpenAIRE

    2014-01-01

    Chitosan (CS)/Polyethylene oxide (PEO) and Polyvinyl alcohol (PVA)/ Aloe vera (AVG) based electrospun nanofibers were gathered together by dual electrospinning system. Properties of blended solutions for electrospinning were investigated by measuring viscosity, conductivity and pH values. Morphological characteristics of the nanofibers were analyzed by Scanning Electron Microscopy (SEM). Beadless and uniform nanofibers were obtained through the electrospinning process and the diameters of the...

  14. Studies on tissue culture and rapid propagation of 3 species of Aloe%三种芦荟的组培快繁研究

    Institute of Scientific and Technical Information of China (English)

    赵兴兵; 袁正仿; 郭韫丽; 张苏锋; 何永

    2001-01-01

    库拉索芦荟(Aloe vera L.)、中国芦荟[A.vera L. var.chinensis(Haw.)Berg.]、上农大叶芦荟(A.vera L. var SAC Gu.)三种芦荟的茎段和顶芽外植体在MS附加BA3~4mg/L的培养基上培养,产生大量不定芽.不定芽长大后,在MS附加IBA 1~2 mg/L+NAA 0.2 mg/L的培养基上生根并形成完整植株.生根试管苗经练苗后移入沙床,生长良好.

  15. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions.

    Science.gov (United States)

    Kodiyan, Joyson; Amber, Kyle T

    2015-04-23

    Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula's mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy surrounding the efficacy of calendula in treating and preventing radiodermatitis, the topic warrants further discussion.

  16. In vivo diabetic wound healing effect and HPLC–DAD–ESI–MS/MS profiling of the methanol extracts of eight Aloe species

    Directory of Open Access Journals (Sweden)

    Abeer M. El Sayed

    2016-06-01

    Full Text Available Abstract Genus Aloe, Xanthorrhoeaceae, is well distributed all over Egypt, and many species have been used as medicinal plants; mainly reported to prevent cardiovascular diseases, cancer and diabetes. This study attempts to analyze the secondary metabolites in the methanol extract of the leaves of eight Aloe species; A. vera (L. Burm. f., A. arborescens Mill., A. eru A. Berger, A. grandidentata Salm-Dyck, A. perfoliata L., A. brevifolia Mill., A. saponaria Haw. and A. ferox Mill. growing in Egypt. For this aim HPLC–DAD–MS/MS in negative ion mode was used. Although belonging to the same genus, the composition of each species presented different particularities. Seventy one compounds were identified in the investigated Aloe species, of which cis-p-coumaric acid derivaties, 3,4-O-(E caffeoylferuloylquinic acid and caffeoyl quinic acid hexoside were the most common phenolic acids identified. Aloeresin E and isoaloeresin D, 2'-O-feruloylaloesin were the common anthraquinones identified. Lucenin II, vicenin II, and orientin were the common identified flavonoids in the investigated Aloe species. 6'-Malonylnataloin, aloe-emodin-8-O-glucoside, flavone-6,8-di-C-glucosides could be considered as chemotaxonomic markers for the investigated Aloe species. The eight Aloe species had significant anti-inflammatory activity, in addition to the significant acceleration of diabetic wound healing in rats following topical application of the methanol extracts of their leaves. This is the first simultaneous characterization and qualitative determination of multiple phenolic compounds in Aloe species from locally grown cultivars in Egypt using HPLC–DAD–MS/MS, which can be applied to standardize the quality of different Aloe species and the future design of nutraceuticals and cosmetic preparations.

  17. Aloe in Angola (Asphodelaceae: Alooideae

    Directory of Open Access Journals (Sweden)

    R. R. Klopper

    2009-12-01

    Full Text Available Botanical exploration of Angola was virtually impossible during the almost three-decade-long civil war. With more areas becoming accessible, there is, however, a revived interest in the flora of this country. A total of 27 members of the genus Aloe L. have been recorded for Angola. It is not unlikely that new taxa will be discovered, and that the distribution ranges of others will be expanded now that botanical exploration in Angola has resumed. This manuscript provides a complete taxonomic treatment of the known Aloe taxa in Angola. It includes, amongst other information, identification keys, descriptions and distribution maps.

  18. Role of priming technique in germination parameters of calendula (Calendula officinalis L. seeds

    Directory of Open Access Journals (Sweden)

    Karimi Mahnaz

    2016-01-01

    Full Text Available Seed priming is known for its promoting effects on seed and seedling properties under stress conditions such as salinity, temperature and drought stresses. Poor plant stand establishment is a common problem for marigold production. Therefore, an experiment was conducted to investigate the effect of different seed priming techniques on calendula seed germination and seedling enzyme activity. For this purpose, different seed priming techniques: hydropriming (distilled water, 24 and 72 h, osmopriming (0.02% or 0.04% KNO3 for 24 and 72 h and hormonal priming (100 or 500 mg L-1 GA3 for 24 and 72 h were assessed. Seeds without any treatment were considered as control. Compared with control, 100 mg L-1 GA3 and distilled water for 72 h showed the highest promoting effects on germination percentage. The highest vigor index (525.75 was related to hydropriming (for 24 h treatment. Also, the maximum root length was found with 0.02% KNO3 for 72 h. Catalase significantly increased in the primed seeds. The highest CAT activity and total soluble sugars were found in the seeds primed with 100 mg L-1 GA3 for 72 h. Moreover, the enzyme activity was significantly higher in the distilled water and KNO3 treatments compared with the untreated seeds.

  19. Growth, development and content of flavonoids in calendula (Calendula officinalis L.

    Directory of Open Access Journals (Sweden)

    Isabela Cristina Gomes Honório

    2016-01-01

    Full Text Available Flavonoids, the pharmaco-active compounds studied in this work, in general, are located in the shoots as natural pigments that protect the plants from damage by oxidizing agents. The experiment was conducted in the greenhouse of the Fitotecnia Department, Federal University of Viçosa, Minas Gerais Staty. The experimental design was completely randomized with five treatments and five replications. The treatments were the time of harvest: zero, three, six, nine, and twelve days after anthesis (DAA. The height of the plant (mm, stem diameter (mm, number of leaves and total flavonoid content (%, w w-1 were determined. We developed growth curves for calendula with growth behavior assessed through time for height, stem diameter and number of leaves. The time of the harvest season positively influenced the total flavonoid contents and the average productivity of flowers, and the highest content of flavonoids was found three DAA. The average productivity of the flowers and the accumulation of flavonoids were heterogeneous among harvest times (0, 3, 6, 9 and 12 days after anthesis. The highest yield of flavonoids was obtained three days after anthesis; therefore, a harvest of the inflorescences is recommended every three days.

  20. 芦荟浸泡酒的工艺研究%Technology of aloe steeping wine

    Institute of Scientific and Technical Information of China (English)

    何林; 刘晓娟; 赵力超; 刘欣

    2012-01-01

    以库拉索芦荟为原料,以浓香型的蒸馏白酒为基酒,对芦荟浸泡酒的工艺进行了研究。在单因素实验基础上,利用正交实验研究芦荟添加量、超声波萃取时间、陈酿时间对芦荟浸泡酒感官品质和芦荟多糖含量的影响以确定最佳工艺条件,并进行了后期调味实验。结果表明:芦荟添加量90 g/100 mL,超声波萃取时间10 min,陈酿时间15 d,甜菊糖苷添加量0.08 g/100 mL时,得到的芦荟浸泡酒品质最佳。%Aloe vera was used as raw material and strong aromatic Chinese spirits was used as base liquor. The technology of aloe steeping wine was studied. On the base of single factor test, in order to obtain the optional technology conditions, orthogonal tests were done. The influence of addition of aloe, ultrasonic extraction time and aging time on thesensory quality and polysaccharide content was studied. At the later stage, the seasoning experiment was done. The results showed that: the addition of aloe 90 g/100 mL, the ultrasonic extraction time 10 min, the aging time 15 d, the addition of of stevioside 0.08 g/100 mL, the quality of aloe steeping wine was best.

  1. Aloe sterol supplementation improves skin elasticity in Japanese men with sunlight-exposed skin: a 12-week double-blind, randomized controlled trial

    Directory of Open Access Journals (Sweden)

    Tanaka M

    2016-11-01

    Full Text Available Miyuki Tanaka,1 Yuki Yamamoto,2 Eriko Misawa,1 Kazumi Nabeshima,1 Marie Saito,1 Koji Yamauchi,1 Fumiaki Abe,1 Fukumi Furukawa2 1Functional Food Ingredients Department, Food Ingredients & Technology Institute, Morinaga Milk Industry Co., Ltd., Zama, Kanagawa, 2Department of Dermatology, Wakayama Medical University, Kimiidera, Wakayama, Japan Background/objective: Recently, it was confirmed that the daily oral intake of plant sterols of Aloe vera gel (Aloe sterol significantly increases the skin barrier function, moisture, and elasticity in photoprotected skin. This study aimed to investigate whether Aloe sterol intake affected skin conditions following sunlight exposure in Japanese men. Methods: We performed a 12-week, randomized, double-blind, placebo-controlled study to evaluate the effects of oral Aloe sterol supplementation on skin conditions in 48 apparently healthy men (age range: 30–59 years; average: 45 years. The subjects were instructed to expose the measurement position of the arms to the sunlight outdoors every day for 12 weeks. The skin parameters were measured at 0 (baseline, 4, 8, and 12 weeks. Results: Depending on the time for the revelation of the sunlight, the b* value and melanin index increased and the skin moisture decreased. After taking an Aloe sterol tablet daily for 12 weeks, the skin elasticity index (R2, R5, and R7 levels were significantly higher than the baseline value. There were no differences between the groups in these skin elasticity values. In the subgroup analysis of subjects aged <46 years, the change in the R5 and R7 was significantly higher in the Aloe group than in the placebo group at 8 weeks (P=0.0412 and P=0.0410, respectively. There was a difference in the quantity of sun exposure between each subject, and an additional clinical study that standardizes the amount of ultraviolet rays is warranted. No Aloe sterol intake-dependent harmful phenomenon was observed during the intake period

  2. Comparative evaluation of the effect of chlorhexidine and Aloe barbadensis Miller (Aloe vera on dentin stabilization using shear bond testing

    Directory of Open Access Journals (Sweden)

    Dakshita Joy Sinha

    2016-01-01

    Conclusion: CHX and A. barbadensis Miller, as pretreatment agents of acid demineralized dentin collagen, has no adverse effect on the immediate shear bond strength of a two-step etch and rinse adhesive to dentin.

  3. Quality Characteristics, Nutraceutical Profile, and Storage Stability of Aloe Gel-Papaya Functional Beverage Blend

    Directory of Open Access Journals (Sweden)

    Pushkala Ramachandran

    2014-01-01

    Full Text Available Aloe vera gel, well known for its nutraceutical potential, is being explored as a functional ingredient in a wide array of health foods and drinks. Processing of exotic fruits and herbal botanicals into functional beverage is an emerging sector in food industry. The present study was undertaken to develop a spiced functional RTS beverage blend using Aloe gel (AG and papaya. Aloe gel (30%, papaya pulp (15%, spice extract (5%, and citric acid (0.1% were mixed in given proportion to prepare the blend with TSS of 15 °Brix. The product was bottled, pasteurized, and stored at room temperature. The quality characteristics and storage stability of the spiced beverage blend (SAGPB were compared with spiced papaya RTS beverage (SPB. Periodic analysis was carried out up to five months for various physicochemical parameters, sugar profile, bioactive compounds, microbial quality, instrumental color, and sensory acceptability. The SAGPB exhibited superior quality characteristics compared to SPB both in fresh and in stored samples. The SPB was acceptable up to four months and SAGPB for five months. The results indicate that nutraceutical rich AG could be successfully utilized to develop functional fruit beverages with improved quality and shelf life.

  4. The addition of rosehip oil to Aloe gels improves their properties as postharvest coatings for maintaining quality in plum.

    Science.gov (United States)

    Martínez-Romero, Domingo; Zapata, Pedro J; Guillén, Fabián; Paladines, Diego; Castillo, Salvador; Valero, Daniel; Serrano, María

    2017-02-15

    The effect of Aloe vera gel (AV) and Aloe arborescens gel (AA) alone or in combination with rosehip oil (RO) at 2% on ethylene production, respiration rate, quality parameters, bioactive compounds and antioxidant activity during plum postharvest storage was studied. Coated plums showed a delay in ethylene production and respiration rate at 20°C and during cold storage and subsequent shelf life, the main effect being observed for those fruits coated with AA+RO. Quality parameters such as softening, colour and maturity index was also delayed during storage by the use of the coatings, which led to a 2-fold increase in plum storability. Accumulation of bioactive compounds was also delayed although at the end of the experiment the content of bioactive compounds was higher than those found for control fruits at the estimated shelf life. The most effective coating for maintaining plum quality and bioactive compounds was AA+RO.

  5. Characterization of green synthesized nano-formulation (ZnO-A. vera) and their antibacterial activity against pathogens.

    Science.gov (United States)

    Qian, Yiguang; Yao, Jun; Russel, Mohammad; Chen, Ke; Wang, Xiaoyu

    2015-03-01

    The application of nanotechnology in medicine has recently been a breakthrough in therapeutic drugs formulation. This paper presents the structural and optical characterization of a new green nano-formulation (ZnO-Aloe vera) with considerable antibacterial activity against pathogenic bacteria. Its particle structure, size and morphology were characterized by XRD, TEM and SEM. And optical absorption spectra and photoluminescence were measured synchronously. Their antibacterial activity against Escherichia coli and Staphylococcus aureus was also investigated using thermokinetic profiling and agar well diffusion method. The nano-formulation is spherical shape and hexagonal with a particle size ranging from 25 to 65 nm as well as an increased crystallite size of 49 nm. For antibacterial activity, the maximum inhibition zones of ZnO and ZnO+A. vera are 18.33 and 26.45 mm for E. coli, 22.11 and 28.12 mm for S. aureus (pantibacterial effect against S. aureus almost at all concentration and against E. coli at 15 and 25mg/L. ZnO+A. vera nano-formulation is much more toxic against S. aureus than E. coli, with an IC50 of 13.12 mg/L and 21.31 mg/L, respectively. The overall results reveal that the ZnO-A. vera nano-formulation has good surface energy, crystallinity, transmission, and enriched antibacterial activities. Their antibacterial properties are possibly relevant to particle size, microstructural ionization, the crystal formation and the Gram property of pathogens. This ZnO-A. vera nano-formulation could be utilized effectively as a spectral and significant antibacterial agent for pathogens in future medical and environmental concerns.

  6. Calendula officinalis: Central depressive effect and subacute toxicity

    OpenAIRE

    2009-01-01

    Flores da Calendula officinalis L. (calêndula) vêm sendo utilizadas popularmente como cicatrizante, antiinflamatório e sedativo, entre outras atividades. Os principais componentes químicos encontrados nas flores são óleos essenciais, ácido salicílico, carotenóides, flavonóides, taninos e saponinas triterpênicas. Atividades ansiolítica e analgésica foram relatadas em plantas que apresentam flavonóides em sua composição. Nesse trabalho a atividade do extrato etanólico das flores da ...

  7. Impact of exogenous salicylic acid on growth and ornamental characteristics of calendula (Calendula officinalis L. under salinity stress

    Directory of Open Access Journals (Sweden)

    Bayat H.

    2012-04-01

    Full Text Available Application of salicylic acid (SA as a phytohormone has been increased due to resistance to stresses such as salt stress. Pot experiments were conducted to determine the effect of exogenous salicylic acid application on growth and ornamental characteristics of calendula grown under salt stress and greenhouse conditions. For this purpose a factorial experiment based on completely randomized design was conducted with 3 levels of SA (0 (control, 1, 2 mM and 3 levels of NaCl (0, 100 and 200 mM with 4 replications. At flowering stage, SA was applied with spraying two times in two week intervals. NaCl was also applied as drench (200 ml per pot in two day intervals. The results showed that salinity decreased the growth, Chlorophyll reading values, flower number per plant and flower diameter. However, foliar applications of SA resulted in greater root, shoot and total dry weight, plant height and leaf area of calendula plants under salt stress. The highest chlorophyll reading values was obtained from 2.00 mM SA application in all NaCl treatments. Salinity decreased number of flower per plant and flower diameter as ornamental characteristics; however SA increased them under salinity stress. Plants treated with 1.00 mM SA had the highest flower diameter at 100 and 200 mM of NaCl. Electrolyte leakage increased by salinity, however foliar application of SA significantly reduced electrolyte leakage under salt stress. Based on the present results, foliar application of SA treatments can ameliorate the negative effects of salinity on the growth and ornamental characteristics of calendula plants.

  8. Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1

    Science.gov (United States)

    Deng, Kan; Jiang, Chunling; Fu, Mingui; Guo, Chunlan; Wang, Xiaolei; Wang, Xin; Meng, Fanjing; Yang, Shaoguo; Deng, Keyu

    2016-01-01

    Little work is done to develop Aloe vera (AV) using probiotics. To explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of Aloe fermentation supernatant were evaluated in vitro. Our results indicated that the Aloe fermentation supernatant fermented by Lactobacillus plantarum HM218749.1 had very strong scavenging capacities of the DPPH (86%), O2•− (85%), •OH (76%), and Fe2+ chelation (82%) and reducing powers (242.5 mg/L), and the inhibition zones for Salmonella typhimurium, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Listeria monocytogenes, S. dysenteriae 301, Staphylococcus aureus Cowan1, and Propionibacterium acnes were 16, 15, 19, 20, 21, 20, and 27 mm. Moreover, the low concentration of Aloe fermentation supernatant had significantly reduced the production of IL-1β, TNF-α, and IL-6 in both mRNA and protein levels (P < 0.01). Therefore, the Aloe fermentation supernatant can be used as functional beverage or cosmetic ingredients to guard human intestinal health, delaying senescence, and prevent chronic diseases. PMID:27493450

  9. Evaluation of the Antioxidative, Antibacterial, and Anti-Inflammatory Effects of the Aloe Fermentation Supernatant Containing Lactobacillus plantarum HM218749.1

    Directory of Open Access Journals (Sweden)

    Meixiu Jiang

    2016-01-01

    Full Text Available Little work is done to develop Aloe vera (AV using probiotics. To explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of Aloe fermentation supernatant were evaluated in vitro. Our results indicated that the Aloe fermentation supernatant fermented by Lactobacillus plantarum HM218749.1 had very strong scavenging capacities of the DPPH (86%, O2•- (85%, OH• (76%, and Fe2+ chelation (82% and reducing powers (242.5 mg/L, and the inhibition zones for Salmonella typhimurium, Salmonella enteritidis, Shigella flexneri, Escherichia coli, Listeria monocytogenes, S. dysenteriae 301, Staphylococcus aureus Cowan1, and Propionibacterium acnes were 16, 15, 19, 20, 21, 20, and 27 mm. Moreover, the low concentration of Aloe fermentation supernatant had significantly reduced the production of IL-1β, TNF-α, and IL-6 in both mRNA and protein levels (P<0.01. Therefore, the Aloe fermentation supernatant can be used as functional beverage or cosmetic ingredients to guard human intestinal health, delaying senescence, and prevent chronic diseases.

  10. Hepatoprotective potential of Aloe barbadensis Mill. against carbon tetrachloride induced hepatotoxicity.

    Science.gov (United States)

    Chandan, B K; Saxena, A K; Shukla, Sangeeta; Sharma, Neelam; Gupta, D K; Suri, K A; Suri, Jyotsna; Bhadauria, M; Singh, B

    2007-05-22

    Aloe barbadensis Mill. Syn. Aloe vera Tourn. ex Linn.(Liliaceae) has been used in variety of diseases in traditional Indian system of medicine in India and its use for hepatic ailments is also documented. In the present study an attempt has been made to validate its hepatoprotective activity. The shade dried aerial parts of Aloe barbadensis were extracted with petroleum ether (AB-1), chloroform (AB-2) and methanol (AB-3). The plant marc was extracted with distilled water (AB-4). All the extracts were evaluated for hepatoprotective activity on limited test models as hexobarbitone sleep time, zoxazolamine paralysis time and marker biochemical parameters. AB-1 and AB-2 were observed to be devoid of any hepatoprotective activity. Out of two active extracts (AB-3 and AB-4), the most active AB-4 was studied in detail. AB-4 showed significant hepatoprotective activity against CCl4 induced hepatotoxicity as evident by restoration of serum transaminases, alkaline phosphatase, bilirubin and triglycerides. Hepatoprotective potential was confirmed by the restoration of lipid peroxidation, glutathione, glucose-6-phosphatase and microsomal aniline hydroxylase and amidopyrine N-demethylase towards near normal. Histopathology of the liver tissue further supports the biochemical findings confirming the hepatoprotective potential of AB-4. The present study shows that the aqueous extract of Aloe barbadensis is significantly capable of restoring integrity of hepatocytes indicated by improvement in physiological parameters, excretory capacity (BSP retention) of hepatocytes and also by stimulation of bile flow secretion. AB-4 did not show any sign of toxicity up to oral dose of 2 g/kg in mice.

  11. A Review of the Use of Topical Calendula in the Prevention and Treatment of Radiotherapy-Induced Skin Reactions

    Directory of Open Access Journals (Sweden)

    Joyson Kodiyan

    2015-04-01

    Full Text Available Calendula is a topical agent derived from a plant of the marigold family Calendula Officinalis. Containing numerous polyphenolic antioxidants, calendula has been studied in both the laboratory and clinical setting for the use in treating and preventing radiation induced skin toxicity. Despite strong evidence in the laboratory supporting calendula’s mechanism of action in preventing radiation induced skin toxicity, clinical studies have demonstrated mixed results. In light of the controversy surrounding the efficacy of calendula in treating and preventing radiodermatitis, the topic warrants further discussion.

  12. Studies on spasmogenic and spasmolytic activities of Calendula officinalis flowers.

    Science.gov (United States)

    Bashir, Samra; Janbaz, Khalid H; Jabeen, Qaiser; Gilani, Anwar H

    2006-10-01

    The aqueous-ethanol extract of Calendula officinalis flowers (Co.Cr) was studied for its possible spasmolytic and spasmogenic effects in isolated gut preparations. In rabbit jejunum, Co.Cr caused a dose-dependent (0.03-3.0 mg/mL) relaxation of spontaneous and K+-induced contractions, suggestive of calcium channel blockade (CCB). In a few preparations, a mild non-reproducible spasmogenic effect was observed at lower doses, followed by relaxation. The CCB effect was confirmed when pretreatment of the jejunum preparations with Co.Cr produced a dose-dependent rightward shift in the Ca(++) dose-response curves, similar to that of verapamil. Activity-directed fractionation revealed that the spasmolytic activity of the plant was concentrated in its organic fractions. The aqueous fraction exhibited a marked atropine sensitive spasmogenic effect but was found to be devoid of any spasmolytic effect. These data indicate that the crude extract of Calendula officinalis flowers contains both spasmolytic and spasmogenic constituents, exhibiting these effects through calcium channel blocking and cholinergic activities and this study provides a scientific base for its traditional use in abdominal cramps and constipation.

  13. Effect of Calendula officinalis cream on achilles tendon healing.

    Science.gov (United States)

    Aro, A A; Perez, M O; Vieira, C P; Esquisatto, M A M; Rodrigues, R A F; Gomes, L; Pimentel, E R

    2015-02-01

    In recent years, the scientific community has undertaken research on plant extracts, searching for compounds with pharmacological activities that can be used in diverse fields of medicine. Calendula officinalis L. is known to have antioxidant, anti-inflammatory, antibacterial, and wound healing properties when used to treat skin burns. Therefore, the purpose of this study was to analyze the effects of C. officinalis on the initial phase of Achilles tendon healing. Wistar rats were separated in three groups: Calendula (Cal)-rats with a transected tendon were treated with topical applications of C. officinalis cream and then euthanized 7 days after injury; Control (C)-rats were treated with only vehicle after transection; and Normal (N)-rats without tenotomy. Higher concentrations of hydroxyproline (an indicator of total collagen) and non-collagenous proteins were observed in the Cal group in relation to the C group. Zymography showed no difference in the amount of the isoforms of metalloproteinase-2 and of metalloproteinase-9, between C and Cal groups. Polarization microscopy images analysis showed that the Cal group presented a slightly higher birefringence compared with the C group. In sections of tendons stained with toluidine blue, the transected groups presented higher metachromasy as compared with the N group. Immunocytochemistry analysis for chondroitin-6-sulfate showed no difference between the C and Cal groups. In conclusion, the topical application of C. officinalis after tendon transection increases the concentrations of collagen and non-collagenous proteins, as well as the collagen organization in the initial phase of healing.

  14. Optimization of the preparation of pectin from Aloe using a Box-Behnken design.

    Science.gov (United States)

    Geng, Lijing; Zhou, Wei; Qu, Xingyuan; Chen, Weijie; Li, Yingchang; Liu, Chang; Sun, Jing; Yu, Xiaolei; Wang, Hui; Zhang, Zhen; Li, Jing; Wang, Lijie

    2014-05-25

    The extraction condition of pectin from Aloe vera barbadensis Mill was optimized by a Box-Behnken design. The effect of parameters of extraction water proportion (EWP), extraction pH (EpH), extraction temperature (ETe), extraction time (ETi), alcohol precipitation pH (APpH)and alcohol precipitation temperature (APTe) on the extraction yield of pectin was investigated by a software of Design Export 8.0.5b. The maximum extraction yield was obtained with the EWP of 20:1, EpH of 1.5, ETe of 90°C, ETi of 120min, APpH of 3.0 and APTe of 50°C, which was consistent with the experimental value. We also found out that the pectin content decreased gradually during storage and sucrose concentration had a significant impact on the viscosity of pectin.

  15. 芦荟的开发利用%DEVELOPING AND USING OF ALOE VERA

    Institute of Scientific and Technical Information of China (English)

    聂凌鸿

    2006-01-01

    芦荟及其制品已在化工、医药、美容、保健、食品等领域广为应用.对芦荟品种、芦荟的活性成分及其功效进行了概述,系统讨论和总结了国内外的芦荟开发利用进展,并从化妆品、药品和保健食品等方面论述了芦荟的开发利用.

  16. Evaluation of Calendula mucilage as a mucoadhesive and controlled release component in buccal tablets.

    Science.gov (United States)

    Sabale, V; Patel, V; Paranjape, A

    2014-01-01

    Mucoadhesive drug delivery systems were developed to sustain drug delivery via various mucus membranes for either local or systemic delivery of poorly absorbed drugs such as peptides and proteins as well as drugs that are subjected to high first-pass metabolism. The present study was undertaken to use isolated Calendula mucilage as a mucoadhesive agent and to formulate controlled release buccoadhesive tablets with an intention to avoid hepatic first-pass metabolism as well as to enhance residence time of drug in the buccal cavity. The mucilage was isolated from the Calendula petals by aqueous extraction method and characterized for various physiochemical parameters as well as for its adhesive properties. By using direct compression technique, tablets were prepared containing dried mucilage and chlorpheniramine maleate (CPM) as a model drug. Three batches of tablets were prepared and evaluated containing three mucoadhesive components namely Methocel K4M, Carbopol 974P and isolated Calendula mucilage in 16.66%, 33.33 % and 50 % (1:2:3 ratio) resulting in 9 different formulations. FTIR studies between mucilage and CPM suggested the absence of a chemical interaction between CPM and Calendula mucilage. The results of the study showed that the isolated mucilage had good physicochemical and morphological characteristics and tablets conformed to the pharmacopoeial specifications. Also in vitro release studies showed controlled action of drug with increasing the concentration of the isolated Calendula mucilage as a mucoadhesive agent in the formulations. Permeability studies indicated that permeability behavior was not statistically different (P>0.05) by changing the mucoadhesive component. The formulated mucoadhesive tablets for buccal administration containing 75 mg Calendula mucilage showed controlled drug release. Thus, mucoadhesive natural Calendula mucilage based buccal tablets for controlled release were successfully formulated.

  17. Carotenoid isomerase is key determinant of petal color of Calendula officinalis.

    Science.gov (United States)

    Kishimoto, Sanae; Ohmiya, Akemi

    2012-01-02

    Orange petals of calendula (Calendula officinalis) accumulate red carotenoids with the cis-configuration at the C-5 or C-5' position (5-cis-carotenoids). We speculated that the orange-flowered calendula is a carotenoid isomerase (crtiso) loss-of-function mutant that impairs the cis-to-trans conversion of 5-cis-carotenoids. We compared the sequences and enzyme activities of CRTISO from orange- and yellow-flowered calendulas. Four types of CRTISO were expressed in calendula petals. The deduced amino acid sequence of one of these genes (CoCRTISO1) was different between orange- and yellow-flowered calendulas, whereas the sequences of the other three CRTISOs were identical between these plants. Analysis of the enzymatic activities of the CoCRTISO homologs showed that CoCRTISO1-Y, which was expressed in yellow petals, converted carotenoids from the cis-to-trans-configuration, whereas both CoCRTISO1-ORa and 1-ORb, which were expressed in orange petals, showed no activity with any of the cis-carotenoids we tested. Moreover, the CoCRTISO1 genotypes of the F2 progeny obtained by crossing orange and yellow lines linked closely to petal color. These data indicate that CoCRTISO1 is a key regulator of the accumulation of 5-cis-carotenoids in calendula petals. Site-directed mutagenesis showed that the deletion of Cys-His-His at positions 462-464 in CoCRTISO1-ORa and a Gly-to-Glu amino acid substitution at position 450 in CoCRTISO1-ORb abolished enzyme activity completely, indicating that these amino acid residues are important for the enzymatic activity of CRTISO.

  18. Wound healing activity of flower extract of Calendula officinalis.

    Science.gov (United States)

    Preethi, Korengath C; Kuttan, Ramadasan

    2009-01-01

    The effects of oral and topical application of Calendula officinalis flower extract on excision wounds made in rats were checked. The parameters assessed were the days needed for re-epithelization and percentage of wound closure. The hydroxy proline and hexosamine content in the granuloma tissue of the wound was also measured. The percentage of wound closure was 90.0% in the extract-treated group, whereas the control group showed only 51.1% on the eighth day of wounding (p < .01). The days needed for re-epithelization were 17.7 for the control animals; extract treatment at a dose of 20 or 100 mg/kg b.wt reduced the period to 14 and 13 days, respectively. A significant increase was observed in the hydroxy proline and hexosamine content in the extract-treated group compared with the untreated animals. The data indicate potent wound healing activity ofC. officinalis extract.

  19. Toxicological studies on hydroalcohol extract of Calendula officinalis L.

    Science.gov (United States)

    Silva, Erick J R; Gonçalves, Eduardo S; Aguiar, Fábio; Evêncio, Liriane B; Lyra, Mariana M A; Coelho, Maria Cristina O C; Fraga, Maria do Carmo C A; Wanderley, Almir G

    2007-04-01

    The hydroalcohol extract (HAE) of Calendula officinalis L. was evaluated for its acute toxicity by the oral route in rats and mice and for the subacute effect on hematological, biochemical and morphologic parameters in rats. In the acute toxicity test, HAE failed to cause death in the animals after administration of oral doses up to 5.0 g/kg. Oral treatment with HAE at 0.025, 0.25, 0.5 and 1.0 g/kg did not induce hematological alterations when compared with the control group. In the biochemical parameters, there was an increase in blood urea nitrogen (BUN) and in alanine transaminase (ALT) levels. Morphological examination of the brain, kidney and heart did not show any alteration. However, inflammatory sites were found in the lung and liver, which were associated, respectively, with oral gavage and a possible hepatotoxic effect. HAE was non-toxic in rats, although there was evidence of renal and liver overload.

  20. DETERMINATION OF ANTIMICROBIAL ACTIVITY OF EXTRACTS OF CALENDULA OFFICINALIS FLOWERS

    Directory of Open Access Journals (Sweden)

    P. V. Afanasyeva

    2016-01-01

    Full Text Available Pot marigold (Calendula officinalis L. is one of the most popular medicinal plants in the Russian Federation and abroad. The wide range of pharmacological activity of this medicinal plant is determined by carotenoids, flavonoids, saponins. These biologically active substances give total therapeutic effect of flowers of Calendula officinalis and medicines on base of pot marigold. This paper discusses the results of comparative investigations for a determination of antimicrobial activity of aqueous and aqueous- alcoholic extracts from pot marigold flowers. Detection of the minimum inhibitory concentration (MIC was carried out by using the method of double serial dilutions in broth. The following microorganisms were used as test cultures: Bacillus cereus, Candida albicans, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The study showed that the widest spectrum of antibacterial activity has water extract of pot marigold flowers. As for Pseudomonas aeruginosa the most active medicine is tincture (1:10 with 70% alcohol. As for Escherichia coli the only phytopharmaceutical – water extract of marigold flowers, reveals antimicrobial activity. Against Bacillus cereus the most effective properties was indicated for tincture (1:5 with 70% ethanol and the liquid extract (1:2 with 70% alcohol. In case of Candida albicans, tincture (1:10 with 70% alcohol exhibited the highest activity.

  1. Usage of Calendula officinalis in the prevention and treatment of radiodermatitis: a randomized double-blind controlled clinical trial

    Directory of Open Access Journals (Sweden)

    Franciane Schneider

    2015-04-01

    Full Text Available OBJECTIVE To evaluate the efficacy of Calendula officinalis in relation to Essential Fatty Acids for the prevention and treatment of radiodermatitis. METHOD This is a randomized double-blind controlled clinical trial with 51 patients with head and neck cancer in radiotherapy treatment divided into two groups: control (27 and experimental (24. RESULTS There is statistically significant evidence (p-value = 0.0120 that the proportion of radiodermatitis grade 2 in Essential Fatty Acids group is higher than Calendula group. Through the Kaplan-Meier survival curve we observed that Essential Fatty Acids group has always remained below the Calendula group survival curve, due to the lower risk of developing radiodermatitis grade 1, which makes the usage of Calendula more effective, with statistical significance (p-value = 0.00402. CONCLUSION Calendula showed better therapeutic response than the Essential Fatty Acids in the prevention and treatment of radiodermatitis. Brazilian Registry of Clinical Trials: RBR-237v4b.

  2. Comparing the effects of Calendula officinalis and clotrimazole on vaginal Candidiasis: A randomized controlled trial.

    Science.gov (United States)

    Saffari, Elnaz; Mohammad-Alizadeh-Charandabi, Sakineh; Adibpour, Mohammad; Mirghafourvand, Mojgan; Javadzadeh, Yousef

    2016-11-23

    This triple-blind trial examined the effects of Calendula officinalis vaginal cream on the treatment of vaginal Candidiasis (primary outcome) and sexual function (secondary outcome). Married women aged 18-45 years with vaginal Candidiasis (n = 150) were recruited from April to October 2014 and randomized into Calendula and clotrimazole groups, using 5-g vaginal cream every night for seven nights. Clinical and laboratory assessments were conducted at 10-15 and 30-35 days after intervention and the female sexual function index was assessed at 30-35 days. Six women were lost to follow-up. The frequency of testing negative for Candidiasis in the Calendula group was significantly lower at the first (49% vs. 74%; odds ratio (OR) 0.32; 95% confidence interval (CI) 0.16-0.67) but higher at the second (77% vs. 34%; OR 3.1; 95% CI 1.5-6.2) follow-up compared to the clotrimazole group. The frequency of most signs and symptoms were almost equal in the two groups at the first follow-up, but were significantly lower in the Calendula group at the second follow-up. Sexual function had almost equal significant improvement in both groups. Calendula vaginal cream appears to have been effective in the treatment of vaginal Candidiasis and to have a delayed but greater long-term effect compared to clotrimazole.

  3. 芦荟蜂蜜饮料的研制%Study of the Processing of Aloe and Honey Juice

    Institute of Scientific and Technical Information of China (English)

    周治德; 李桂银; 黄燕萍

    2011-01-01

    A process of Aloe and honey compound beverage was studied. The main ingredients are aloe and hon- ey. Citric acid, sugar, sodium iso-VC are used as food additives. Through a scientific study, a health benefits natural beverage was produced. In this paper, the production process and formulation was studied and a reasonable processing parameters were investigated. Orthogonal experimental results showed that the best formula was a combination of 20% of aloe vera juice, 12% of honey, 3% of sucrose, 0. 15% of citric acid. The best Stabilizers formula were 0.20% CMC with 0. 10% xanthan gum.%以库拉索芦荟和蜂蜜为主要原料,以柠檬酸、蔗糖、异Vc钠等为辅料,加工制成复合型饮料。正交实验表明,芦荟蜂蜜饮料的适宜配方为:芦荟汁20%,蜂蜜12%,白砂糖3%,柠檬酸0.15%。稳定剂为0.20羧甲基纤维素(CMC)+0.10%黄原胶。

  4. Oligosaccharides isolated from Agave vera cruz

    NARCIS (Netherlands)

    Vliegenthart, J.F.G.; Dorland, L.; Kamerling, J.P.; Satyanarayana, M.N.

    1977-01-01

    The structures of naturally occurring and enzymically synthesized oligosaccharides, consisting of fructose and glucose residues and having d.p. 3–8, in the stem of Agave vera cruz have been investigated by using methylation analysis, mass spectrometry, and p.m.r. spectroscopy. The naturally occurrin

  5. Progress of the basic Research and Application Research of the Aloe Leaves%芦荟属植物叶基础与应用研究进展

    Institute of Scientific and Technical Information of China (English)

    叶胜明

    2012-01-01

    This article describes the research progress of aloe from the structure of aloe vera leaves,its chemical composition,function and application research.The main chemical components of aloe is anthraquinones and their derivatives,etc.Its main function is that it can make people more beautiful and healthier,inhibit bacterial breeding,reduce people's blood fat,and resist tumors.Now the products of aloe can be divided into three categories: health food,medicines and cosmetics.%从芦荟叶片的结构、化学成分、功效、应用性研空四个方面来阐述芦荟的研究进展。芦荟的主要活性成分为蒽醌类及其衍生物等,其主要的功效包括美容、保健、抑菌、降血脂、抗肿瘤等,目前芦荟开发出来的产品可分为三大类,分别是保健食品类、药品类和化妆品类。

  6. Evaluation of chemical enhancement on phytoremediation effect of Cd-contaminated soils with Calendula officinalis L.

    Science.gov (United States)

    Liu, Jianv; Zhou, Qixing; Wang, Song

    2010-07-01

    The popular ornamental plant Calendula officinalis L was studied for its potential application in the phytoremediation of cadmium (Cd)-contaminated soils. Enhancements to the Cd accumulation by the application of sodium dodecyl sulfate (SDS), ethylenediaminetriacetic acid (EDTA) and ethylenegluatarotriacetic acid (EGTA) to the soil were investigated. Under these chemically enhanced treatments, EDTA was observed to be toxic to the plants leading to retarded growth. However, the application of SDS and/or EGTA was shown to result in significantly increased plant biomass (p Calendula officinalis L. for applications of phytoremediation of Cd-contaminated sites.

  7. The Virtual Environment for Reactor Applications (VERA). Design and architecture☆

    Science.gov (United States)

    Turner, John A.; Clarno, Kevin; Sieger, Matt; Bartlett, Roscoe; Collins, Benjamin; Pawlowski, Roger; Schmidt, Rodney; Summers, Randall

    2016-12-01

    VERA, the Virtual Environment for Reactor Applications, is the system of physics capabilities being developed and deployed by the Consortium for Advanced Simulation of Light Water Reactors (CASL). CASL was established for the modeling and simulation of commercial nuclear reactors. VERA consists of integrating and interfacing software together with a suite of physics components adapted and/or refactored to simulate relevant physical phenomena in a coupled manner. VERA also includes the software development environment and computational infrastructure needed for these components to be effectively used. We describe the architecture of VERA from both software and numerical perspectives, along with the goals and constraints that drove major design decisions, and their implications. We explain why VERA is an environment rather than a framework or toolkit, why these distinctions are relevant (particularly for coupled physics applications), and provide an overview of results that demonstrate the use of VERA tools for a variety of challenging applications within the nuclear industry.

  8. Biological Activities of Oleanolic Acid Derivatives from Calendula officinalis Seeds.

    Science.gov (United States)

    Zaki, Ahmed; Ashour, Ahmed; Mira, Amira; Kishikawa, Asuka; Nakagawa, Toshinori; Zhu, Qinchang; Shimizu, Kuniyoshi

    2016-05-01

    Phytochemical examination of butanol fraction of Calendula officinalis seeds led to the isolation of two compounds identified as 28-O-β-D-glucopyranosyl-oleanolic acid 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS1) and oleanolic acid 3-O-β-D-glucopyranosyl (1→3)-β-D-glucopyranosiduronic acid (CS2). Biological evaluation was carried out for these two compounds such as melanin biosynthesis inhibitory, hyaluronic acid production activities, anti obesity using lipase inhibition and adipocyte differentiation as well as evaluation of the protective effect against hydrogen peroxide induced neurotoxicity in neuro-2A cells. The results showed that, compound CS2 has a melanin biosynthesis stimulatory activity; however, compound CS1 has a potent stimulatory effect for the production of hyaluronic acid on normal human dermal fibroblast from adult (NHDF-Ad). Both compounds did not show any inhibitory effect on both lipase and adipocyte differentiation. Compound CS2 could protect neuro-2A cells and increased cell viability against H2 O2 . These activities (melanin biosynthesis stimulatory and protective effect against H2 O2 of CS2 and hyaluronic acid productive activities of these triterpene derivatives) have been reported for the first time. Copyright © 2016 John Wiley & Sons, Ltd.

  9. The reproductive strategies of the heterocarpic annual Calendula arvensis (Asteraceae)

    Science.gov (United States)

    Ruiz De Clavijo, E.

    2005-09-01

    Achene polymorphism and various aspects of the reproductive biology of the annual Calendula arvensis L. (Field marigold), were studied to determine the reproductive strategies of the plant. This species normally produces three types of achene: rostrate, cymbiform and annular. Rostrate and cymbiform achenes are larger and heavier than annular achenes, and are adapted to long-range dispersal (by epizoochory and anemochory, respectively). In contrast, annular achenes are smaller in size and weight, and are adapted to short-range dispersal. Achenes germinate over a broad range of temperatures (both in light and in darkness), exhibiting cymbiform achenes the highest germination percentages and annular achenes the lowest under all conditions tested. A fraction of the three types of achenes exhibit dormancy and presumably enter the soil seedbank. Achene types adapted for long-range dispersal (rostrate and cymbif