JRC GMO-Amplicons: a collection of nucleic acid sequences related to genetically modified organisms.

Science.gov (United States)

Petrillo, Mauro; Angers-Loustau, Alexandre; Henriksson, Peter; Bonfini, Laura; Patak, Alex; Kreysa, Joachim

2015-01-01

The DNA target sequence is the key element in designing detection methods for genetically modified organisms (GMOs). Unfortunately this information is frequently lacking, especially for unauthorized GMOs. In addition, patent sequences are generally poorly annotated, buried in complex and extensive documentation and hard to link to the corresponding GM event. Here, we present the JRC GMO-Amplicons, a database of amplicons collected by screening public nucleotide sequence databanks by in silico determination of PCR amplification with reference methods for GMO analysis. The European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF) provides these methods in the GMOMETHODS database to support enforcement of EU legislation and GM food/feed control. The JRC GMO-Amplicons database is composed of more than 240 000 amplicons, which can be easily accessed and screened through a web interface. To our knowledge, this is the first attempt at pooling and collecting publicly available sequences related to GMOs in food and feed. The JRC GMO-Amplicons supports control laboratories in the design and assessment of GMO methods, providing inter-alia in silico prediction of primers specificity and GM targets coverage. The new tool can assist the laboratories in the analysis of complex issues, such as the detection and identification of unauthorized GMOs. Notably, the JRC GMO-Amplicons database allows the retrieval and characterization of GMO-related sequences included in patents documentation. Finally, it can help annotating poorly described GM sequences and identifying new relevant GMO-related sequences in public databases. The JRC GMO-Amplicons is freely accessible through a web-based portal that is hosted on the EU-RL GMFF website. Database URL: http://gmo-crl.jrc.ec.europa.eu/jrcgmoamplicons/. © The Author(s) 2015. Published by Oxford University Press.

Criticism of EFSA's scientific opinion on combinatorial effects of 'stacked' GM plants.

Science.gov (United States)

Bøhn, Thomas

2018-01-01

Recent genetically modified plants tend to include both insect resistance and herbicide tolerance traits. Some of these 'stacked' GM plants have multiple Cry-toxins expressed as well as tolerance to several herbicides. This means that non-target organisms in the environment (biodiversity) will be co-exposed to multiple stressors simultaneously. A similar co-exposure may happen to consumers through chemical residues in the food chain. EFSA, the responsible unit for minimizing risk of harm in European food chains, has expressed its scientific interest in combinatorial effects. However, when new data showed how two Cry-toxins acted in combination (added toxicity), and that the same Cry-toxins showed combinatorial effects when co-exposed with Roundup (Bøhn et al., 2016), EFSA dismissed these new peer-reviewed results. In effect, EFSA claimed that combinatorial effects are not relevant for itself. EFSA was justifying this by referring to a policy question, and by making invalid assumptions, which could have been checked directly with the lead-author. With such approach, EFSA may miss the opportunity to improve its environmental and health risk assessment of toxins and pesticides in the food chain. Failure to follow its own published requests for combinatorial effects research, may also risk jeopardizing EFSA's scientific and public reputation. Copyright © 2017. Published by Elsevier Ltd.

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2016. Scientific opinion on malachite green in food

DEFF Research Database (Denmark)

Petersen, Annette

Malachite green (MG) has been used globally in aquaculture but is not registered for use infood-producing animals in the European Union. The European Commission requested EFSA to evaluatewhether a reference point for action (RPA) of 2 lg/kg for the sum of MG and its major metaboliteleucomalachite...

Kommentarer til SNS for et fælles dansk svar. Public consultation on the working document on general surveillance. Modtaget 02-08-2005, deadline 01-09-2005, svar 01-09-2005

DEFF Research Database (Denmark)

Kjellsson, Gøsta; Damgaard, Christian; Strandberg, Morten Tune

2005-01-01

supplementing Annex VII of Directive 2001/18/EC” med repræsentanter fra de fleste EU-lande (GK deltager for Danmark). Resultaterne af dette arbejde er dog foreløbig fortrolige. Som udgangspunkt vil det være hensigtsmæssigt at få en samlet oversigt over relevante emner for den generelle overvågning af GMO(P) og...... hvilke eksisterende overvågningsprogrammer der kan anvendes (som EFSA dokumentet også anfører, l. 134-167). Det er dog vigtigt at overvågningen ikke bliver for fokuseret på bestemte effekter og at alle relevante observationer og data anvendes i overvågningen. Foruden landbrugs-organisationer bør også...... om det vil være relevant at anvende data for populationsændringer af mobile organismer højere oppe i fødekæden (fx fugle). Det primære sammenligningsgrundlag (”baseline”) for overvågningen af effekter af GMO-afgrøder i dyrkede arealer og de nærmeste omgivelser vil være tilsvarende arealer og afgrøder...

Towards GMO-free landscapes? Identifying driving factors for the establishment of cooperative GMO-free zones in Germany

OpenAIRE

Consmuller, Nicola; Beckmann, Volker; Petrick, Martin

2011-01-01

Since the end of the quasi-moratorium on genetically modified organisms (GMO) in the European Union in 2004, the establishment of GMO-free zones has become an EU wide phenomenon. In contrast to other European countries, Germany follows the concept of cooperative GMO-free zones where neighbouring farmers contractually refrain from GMO cultivation. In this article, we address the question which underlying factors could account for the establishment of cooperative GMO-free zones in Germany. Draw...

The GMO-Nanotech (Dis)Analogy?

Science.gov (United States)

Sandler, Ronald; Kay, W. D.

2006-01-01

The genetically-modified-organism (GMO) experience has been prominent in motivating science, industry, and regulatory communities to address the social and ethical dimensions of nanotechnology. However, there are some significant problems with the GMO-nanotech analogy. First, it overstates the likelihood of a GMO-like backlash against…

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on Dietary Reference Values for carbohydrates and dietary fibre

OpenAIRE

Tetens, Inge

2011-01-01

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) deals with the establishment of Dietary Reference Values for carbohydrates and dietary fibre. Nutritionally, two broad categories of carbohydrates can be differentiated: “glycaemic carbohydrates”, i.e. carbohydrates digested and absorbed in the human small intestine, and „dietary fibre‟, non-digestible carbohydrates passing to the large intestine. In this Opinion, dietary fibre is defined as non-digestible car...

Registration document 2005; Document de reference 2005

Energy Technology Data Exchange (ETDEWEB)

NONE

2005-07-01

This reference document of Gaz de France provides information and data on the Group activities in 2005: financial informations, business, activities, equipments factories and real estate, trade, capital, organization charts, employment, contracts and research programs. (A.L.B.)

GMO konverents Brüsselis / Nastja Pertsjonok

Index Scriptorium Estoniae

Pertsjonok, Nastja

2007-01-01

Brüsselis toimunud GMO-vabade piirkondade konverentsist, kus räägiti GMO-vabade piirkondade liikumisest, GMO-de lubamisest ja keelamisest ning hoiakutest nende suhtes Euroopa Liidus ja mujal maailmas

Semiautomated TaqMan PCR screening of GMO labelled samples for (unauthorised) GMOs.

Science.gov (United States)

Scholtens, Ingrid M J; Molenaar, Bonnie; van Hoof, Richard A; Zaaijer, Stephanie; Prins, Theo W; Kok, Esther J

2017-06-01

In most countries, systems are in place to analyse food products for the potential presence of genetically modified organisms (GMOs), to enforce labelling requirements and to screen for the potential presence of unauthorised GMOs. With the growing number of GMOs on the world market, a larger diversity of methods is required for informative analyses. In this paper, the specificity of an extended screening set consisting of 32 screening methods to identify different crop species (endogenous genes) and GMO elements was verified against 59 different GMO reference materials. In addition, a cost- and time-efficient strategy for DNA isolation, screening and identification is presented. A module for semiautomated analysis of the screening results and planning of subsequent event-specific tests for identification has been developed. The Excel-based module contains information on the experimentally verified specificity of the element methods and of the EU authorisation status of the GMO events. If a detected GMO element cannot be explained by any of the events as identified in the same sample, this may indicate the presence of an unknown unauthorised GMO that may not yet have been assessed for its safety for humans, animals or the environment.

Availability and Market Penetration of GMO Corn and Soybeans

OpenAIRE

Bruce A. Babcock; Michael D. Duffy; Robert Wisner

1999-01-01

The authors survey various types of GMO (genetically modified organism) corn and soybeans, including both pest-resistant and herbicide-resistant varieties, and then evaluate the extent to which farmers have begun to use GMO crops. Figures cited include percentages of acres planted with GMO crops, as well as comparisons of non-GMO versus GMO yields.

New Multiplexing Tools for Reliable GMO Detection

NARCIS (Netherlands)

Pla, M.; Nadal, A.; Baeten, V.; Bahrdt, C.; Berben, G.; Bertheau, Y.; Coll, A.; Dijk, van J.P.; Dobnik, D.; Fernandez-Pierna, J.A.; Gruden, K.; Hamels, S.; Holck, A.; Holst-Jensen, A.; Janssen, E.; Kok, E.J.; Paz, La J.L.; Laval, V.; Leimanis, S.; Malcevschi, A.; Marmiroli, N.; Morisset, D.; Prins, T.W.; Remacle, J.; Ujhelyi, G.; Wulff, D.

2012-01-01

Among the available methods for GMO detection, enforcement and routine laboratories use in practice PCR, based on the detection of transgenic DNA. The cost required for GMO analysis is constantly increasing due to the progress of GMO commercialization, with inclusion of higher diversity of species,

GMOseek: a user friendly tool for optimized GMO testing.

Science.gov (United States)

Morisset, Dany; Novak, Petra Kralj; Zupanič, Darko; Gruden, Kristina; Lavrač, Nada; Žel, Jana

2014-08-01

With the increasing pace of new Genetically Modified Organisms (GMOs) authorized or in pipeline for commercialization worldwide, the task of the laboratories in charge to test the compliance of food, feed or seed samples with their relevant regulations became difficult and costly. Many of them have already adopted the so called "matrix approach" to rationalize the resources and efforts used to increase their efficiency within a limited budget. Most of the time, the "matrix approach" is implemented using limited information and some proprietary (if any) computational tool to efficiently use the available data. The developed GMOseek software is designed to support decision making in all the phases of routine GMO laboratory testing, including the interpretation of wet-lab results. The tool makes use of a tabulated matrix of GM events and their genetic elements, of the laboratory analysis history and the available information about the sample at hand. The tool uses an optimization approach to suggest the most suited screening assays for the given sample. The practical GMOseek user interface allows the user to customize the search for a cost-efficient combination of screening assays to be employed on a given sample. It further guides the user to select appropriate analyses to determine the presence of individual GM events in the analyzed sample, and it helps taking a final decision regarding the GMO composition in the sample. GMOseek can also be used to evaluate new, previously unused GMO screening targets and to estimate the profitability of developing new GMO screening methods. The presented freely available software tool offers the GMO testing laboratories the possibility to select combinations of assays (e.g. quantitative real-time PCR tests) needed for their task, by allowing the expert to express his/her preferences in terms of multiplexing and cost. The utility of GMOseek is exemplified by analyzing selected food, feed and seed samples from a national reference

GMOtrack: generator of cost-effective GMO testing strategies.

Science.gov (United States)

Novak, Petra Krau; Gruden, Kristina; Morisset, Dany; Lavrac, Nada; Stebih, Dejan; Rotter, Ana; Zel, Jana

2009-01-01

Commercialization of numerous genetically modified organisms (GMOs) has already been approved worldwide, and several additional GMOs are in the approval process. Many countries have adopted legislation to deal with GMO-related issues such as food safety, environmental concerns, and consumers' right of choice, making GMO traceability a necessity. The growing extent of GMO testing makes it important to study optimal GMO detection and identification strategies. This paper formally defines the problem of routine laboratory-level GMO tracking as a cost optimization problem, thus proposing a shift from "the same strategy for all samples" to "sample-centered GMO testing strategies." An algorithm (GMOtrack) for finding optimal two-phase (screening-identification) testing strategies is proposed. The advantages of cost optimization with increasing GMO presence on the market are demonstrated, showing that optimization approaches to analytic GMO traceability can result in major cost reductions. The optimal testing strategies are laboratory-dependent, as the costs depend on prior probabilities of local GMO presence, which are exemplified on food and feed samples. The proposed GMOtrack approach, publicly available under the terms of the General Public License, can be extended to other domains where complex testing is involved, such as safety and quality assurance in the food supply chain.

gmo

African Journals Online (AJOL)

2014-06-01

Jun 1, 2014 ... fied some of the advantages of GMOs and also the health concerns. It is clear ... GMO products and post-marketing surveillance for possible negative ... Use of technology for Gene therapy - direct use of. DNA to treat disease.

Forbrugernes modvilje lammer forskning i gmo

DEFF Research Database (Denmark)

Riising, Tina

2005-01-01

GMO kan optimere syrningen af mælk i osteproduktion, men forskningen kan ikke udnyttes Udgivelsesdato: 20 oktober......GMO kan optimere syrningen af mælk i osteproduktion, men forskningen kan ikke udnyttes Udgivelsesdato: 20 oktober...

The central dogma, "GMO" and defective epistemology.

Science.gov (United States)

Tagliabue, Giovanni

2017-10-02

The expression "Genetically Modified Organisms" was coined to indicate a group of agricultural products (mostly crops and vegetables), modified through direct DNA recombination in order to obtain useful phenotypic traits or to inhibit undesirable characteristics. But the border between rDNA ("GMO") and other biotech methods is blurred. Moreover, the ill-assorted group is frequently charged with having peculiar, negative characteristics: many activists, part of the public and a few social science scholars think that "GMOs" are all dubious, even inherently dangerous. However, theoretical justifications of this alleged problematic nature which is supposed to be necessarily linked to the "splicing" of DNA, only when applied to agricultural products, are missing: the only text which tries to go in depth on the subject, an article by biologist Barry Commoner, takes aim at the wrong target, misunderstanding the Central Dogma. "GMO" is a term that has no clear reference, let alone in a detrimental sense. The only attempt to give it epistemological dignity fails.

Recovery in environmental risk assessment at EFSA

NARCIS (Netherlands)

Brock, T.C.M.

2016-01-01

EFSA performs environmental risk assessments (ERAs) for single potential stressors such as plant protection products, genetically modified organisms and feed additives and for invasive alien species that are harmful for plant health. In this risk assessment domain, the EFSA Scientific Committee

Communities of beetles and spiders in the stands of normal and genetically modified maize

Czech Academy of Sciences Publication Activity Database

Habuštová, Oxana; Turanli, Ferit; Spitzer, L.; Růžička, Vlastimil; Doležal, Petr; Sehnal, František

2005-01-01

Roč. 3, - (2005), s. 125-131 ISSN 0208-8703 R&D Projects: GA AV ČR(CZ) KJB6007304 Institutional research plan: CEZ:AV0Z50070508 Keywords : GMO * Bt-maize * agroecosystems Subject RIV: EH - Ecology, Behaviour

A novel GMO biosensor for rapid ultrasensitive and simultaneous detection of multiple DNA components in GMO products.

Science.gov (United States)

Huang, Lin; Zheng, Lei; Chen, Yinji; Xue, Feng; Cheng, Lin; Adeloju, Samuel B; Chen, Wei

2015-04-15

Since the introduction of genetically modified organisms (GMOs), there has been on-going and continuous concern and debates on the commercialization of products derived from GMOs. There is an urgent need for development of highly efficient analytical methods for rapid and high throughput screening of GMOs components, as required for appropriate labeling of GMO-derived foods, as well as for on-site inspection and import/export quarantine. In this study, we describe, for the first time, a multi-labeling based electrochemical biosensor for simultaneous detection of multiple DNA components of GMO products on the same sensing interface. Two-round signal amplification was applied by using both an exonuclease enzyme catalytic reaction and gold nanoparticle-based bio-barcode related strategies, respectively. Simultaneous multiple detections of different DNA components of GMOs were successfully achieved with satisfied sensitivity using this electrochemical biosensor. Furthermore, the robustness and effectiveness of the proposed approach was successfully demonstrated by application to various GMO products, including locally obtained and confirmed commercial GMO seeds and transgenetic plants. The proposed electrochemical biosensor demonstrated unique merits that promise to gain more interest in its use for rapid and on-site simultaneous multiple screening of different components of GMO products. Copyright © 2014 Elsevier B.V. All rights reserved.

Insect communities on maize expressing a Bt-toxin

Czech Academy of Sciences Publication Activity Database

Habuštová, Oxana; Sehnal, František; Hussein, Hany

2005-01-01

Roč. 1, - (2005), s. 9-11 ISSN 1335-258X R&D Projects: GA AV ČR(CZ) KJB6007304 Institutional research plan: CEZ:AV0Z50070508 Keywords : GMO * arthropod communities * Bt maize Subject RIV: EH - Ecology, Behaviour

Genetically modified potatoes expressing Cry 3A protein do not affect aphidophagous coccinellids

Czech Academy of Sciences Publication Activity Database

Kalushkov, P.; Nedvěd, Oldřich

2005-01-01

Roč. 129, č. 8 (2005), s. 401-406 ISSN 0931-2048 R&D Projects: GA ČR(CZ) GA522/02/1507 Grant - others:Bulgarian National Research Fund(BG) B-1105/01 Institutional research plan: CEZ:AV0Z50070508 Keywords : Bacillus thuringiensis * Coccinellidae * GMO Subject RIV: ED - Physiology Impact factor: 0.703, year: 2005

GMDD: a database of GMO detection methods

NARCIS (Netherlands)

Dong, W.; Yang, L.; Shen, K.; Kim, B.; Kleter, G.A.; Marvin, H.J.P.; Guo, R.; Liang, W.; Zhang, D.

2008-01-01

Since more than one hundred events of genetically modified organisms (GMOs) have been developed and approved for commercialization in global area, the GMO analysis methods are essential for the enforcement of GMO labelling regulations. Protein and nucleic acid-based detection techniques have been

[Assessment of allergenicity of genetically modified food crops].

Science.gov (United States)

Schauzu, M; Pöting, A; Rubin, D; Lampen, A

2012-03-01

The placing on the European Union's market of genetically modified crops requires authorization by the European Commission which is based on the proof that the derived foods are as safe as their conventional counterparts. The assessment of potential allergenicity is part of the necessary investigations recommended in the updated Guidance Document of the Scientific Panel on Genetically Modified Organisms (GMO) of the European Food Safety Authority (EFSA), which is based on internationally agreed recommendations. All genetically modified crops which so far have been authorized in the European Union were evaluated by the EFSA GMO Panel which considered it unlikely that their overall allergenicity has been altered.

Timing GMO

DEFF Research Database (Denmark)

Horsbøl, Anders; Lassen, Inger

2011-01-01

events, and observations of this nature leads us to argue that temporality does important ideological and rhetorical work in the text examples discussed. Our data stems from three sources: a focus-group interview with local citizens, a video-recording of an anti-GMO-demonstration in a local town...

EFSA Scientific Committee; Scientific Opinion on Risk Assessment Terminology

DEFF Research Database (Denmark)

Hald, Tine

of improving the expression and communication of risk and/or uncertainties in the selected opinions. The Scientific Committee concluded that risk assessment terminology is not fully harmonised within EFSA. In part this is caused by sectoral legislation defining specific terminology and international standards......The Scientific Committee of the European Food Safety Authority (EFSA) reviewed the use of risk assessment terminology within its Scientific Panels. An external report, commissioned by EFSA, analysed 219 opinions published by the Scientific Committee and Panels to recommend possible ways......, the Scientific Committee concludes that particular care must be taken that the principles of CAC, OIE or IPPC are followed strictly. EFSA Scientific Panels should identify which specific approach is most useful in dealing with their individual mandates. The Scientific Committee considered detailed aspects...

Economic and environmental impacts of a hypothetical global GMO ban

OpenAIRE

Mahaffey, Harrison H

2016-01-01

The objective of this research is to assess the global economic and greenhouse gas emission impacts of GMO crops. This is done by modeling two counterfactual scenarios and evaluating them apart and in combination. The first scenario models the impact of a global GMO ban. The second scenario models the impact of increased GMO penetration. The focus is on the price and welfare impacts, and land use change greenhouse gas (GHG) emissions associated with GMO technologies. Much of the prior work on...

See what you eat--broad GMO screening with microarrays.

Science.gov (United States)

von Götz, Franz

2010-03-01

Despite the controversy of whether genetically modified organisms (GMOs) are beneficial or harmful for humans, animals, and/or ecosystems, the number of cultivated GMOs is increasing every year. Many countries and federations have implemented safety and surveillance systems for GMOs. Potent testing technologies need to be developed and implemented to monitor the increasing number of GMOs. First, these GMO tests need to be comprehensive, i.e., should detect all, or at least the most important, GMOs on the market. This type of GMO screening requires a high degree of parallel tests or multiplexing. To date, DNA microarrays have the highest number of multiplexing capabilities when nucleic acids are analyzed. This trend article focuses on the evolution of DNA microarrays for GMO testing. Over the last 7 years, combinations of multiplex PCR detection and microarray detection have been developed to qualitatively assess the presence of GMOs. One example is the commercially available DualChip GMO (Eppendorf, Germany; http://www.eppendorf-biochip.com), which is the only GMO screening system successfully validated in a multicenter study. With use of innovative amplification techniques, promising steps have recently been taken to make GMO detection with microarrays quantitative.

Development of real-time PCR method for the detection and the quantification of a new endogenous reference gene in sugar beet "Beta vulgaris L.": GMO application.

Science.gov (United States)

Chaouachi, Maher; Alaya, Akram; Ali, Imen Ben Haj; Hafsa, Ahmed Ben; Nabi, Nesrine; Bérard, Aurélie; Romaniuk, Marcel; Skhiri, Fethia; Saïd, Khaled

2013-01-01

KEY MESSAGE : Here, we describe a new developed quantitative real-time PCR method for the detection and quantification of a new specific endogenous reference gene used in GMO analysis. The key requirement of this study was the identification of a new reference gene used for the differentiation of the four genomic sections of the sugar beet (Beta vulgaris L.) (Beta, Corrollinae, Nanae and Procumbentes) suitable for quantification of genetically modified sugar beet. A specific qualitative polymerase chain reaction (PCR) assay was designed to detect the sugar beet amplifying a region of the adenylate transporter (ant) gene only from the species of the genomic section I of the genus Beta (cultivated and wild relatives) and showing negative PCR results for 7 species of the 3 other sections, 8 related species and 20 non-sugar beet plants. The sensitivity of the assay was 15 haploid genome copies (HGC). A quantitative real-time polymerase chain reaction (QRT-PCR) assay was also performed, having high linearity (R (2) > 0.994) over sugar beet standard concentrations ranging from 20,000 to 10 HGC of the sugar beet DNA per PCR. The QRT-PCR assay described in this study was specific and more sensitive for sugar beet quantification compared to the validated test previously reported in the European Reference Laboratory. This assay is suitable for GMO quantification in routine analysis from a wide variety of matrices.

Effect of food processing on plant DNA degradation and PCR-based GMO analysis: a review.

Science.gov (United States)

Gryson, Nicolas

2010-03-01

The applicability of a DNA-based method for GMO detection and quantification depends on the quality and quantity of the DNA. Important food-processing conditions, for example temperature and pH, may lead to degradation of the DNA, rendering PCR analysis impossible or GMO quantification unreliable. This review discusses the effect of several food processes on DNA degradation and subsequent GMO detection and quantification. The data show that, although many of these processes do indeed lead to the fragmentation of DNA, amplification of the DNA may still be possible. Length and composition of the amplicon may, however, affect the result, as also may the method of extraction used. Also, many techniques are used to describe the behaviour of DNA in food processing, which occasionally makes it difficult to compare research results. Further research should be aimed at defining ingredients in terms of their DNA quality and PCR amplification ability, and elaboration of matrix-specific certified reference materials.

Current and New Approaches in GMO Detection: Challenges and Solutions

Directory of Open Access Journals (Sweden)

Marie-Alice Fraiture

2015-01-01

Full Text Available In many countries, genetically modified organisms (GMO legislations have been established in order to guarantee the traceability of food/feed products on the market and to protect the consumer freedom of choice. Therefore, several GMO detection strategies, mainly based on DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR is the method of choice for the enforcement laboratories in GMO routine analysis. However, given the increasing number and diversity of GMO developed and put on the market around the world, some technical hurdles could be encountered with the qPCR technology, mainly owing to its inherent properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification, simultaneous detections of multiple GM targets (e.g., PCR capillary gel electrophoresis, microarray, and Luminex, more accurate quantification of GM targets (e.g., digital PCR, or characterization of partially known (e.g., DNA walking and Next Generation Sequencing (NGS or unknown (e.g., NGS GMO. The benefits and drawbacks of these methods are discussed in this review.

Current and new approaches in GMO detection: challenges and solutions.

Science.gov (United States)

Fraiture, Marie-Alice; Herman, Philippe; Taverniers, Isabel; De Loose, Marc; Deforce, Dieter; Roosens, Nancy H

2015-01-01

In many countries, genetically modified organisms (GMO) legislations have been established in order to guarantee the traceability of food/feed products on the market and to protect the consumer freedom of choice. Therefore, several GMO detection strategies, mainly based on DNA, have been developed to implement these legislations. Due to its numerous advantages, the quantitative PCR (qPCR) is the method of choice for the enforcement laboratories in GMO routine analysis. However, given the increasing number and diversity of GMO developed and put on the market around the world, some technical hurdles could be encountered with the qPCR technology, mainly owing to its inherent properties. To address these challenges, alternative GMO detection methods have been developed, allowing faster detections of single GM target (e.g., loop-mediated isothermal amplification), simultaneous detections of multiple GM targets (e.g., PCR capillary gel electrophoresis, microarray, and Luminex), more accurate quantification of GM targets (e.g., digital PCR), or characterization of partially known (e.g., DNA walking and Next Generation Sequencing (NGS)) or unknown (e.g., NGS) GMO. The benefits and drawbacks of these methods are discussed in this review.

Identifying driving factors for the establishment of cooperative GMO-free zones in Germany

OpenAIRE

Consmuller, Nicola; Beckmann, Volker; Petrick, Martin

2012-01-01

Since the end of the quasi-moratorium on genetically modified organisms (GMO) in the European Union in 2004, the establishment of GMO-free zones has become an EU wide phenomenon. In contrast to other European countries, Germany follows the concept of cooperative GMO-free zones where neighbouring farmers contractually refrain from GMO cultivation. In this article, we address the question which underlying factors could account for the establishment of cooperative GMO-free zones in Germany. Draw...

USA: California rejects mandatory GMO labelling

OpenAIRE

Paull, John

2012-01-01

Buying organic remains the best strategy for US consumers to avoid eating GM food. The voters of California have rejected the proposal to label GMO food. The proposition was narrowly lost, 47% to 53% (4,326,770 ‘Yes’ votes vs. 4,884,961 ‘No’ votes). Proposition 47 was supported by the organic sector but opposed by a coalition of GMO companies and US multinational food companies. Californians were invited to vote into law ‘The California Right to Know Genetically Engineered Food Act’. Section ...

Rapid genetically modified organism (GMO screening of various food products and animal feeds using multiplex polymerase chain reaction (PCR

Directory of Open Access Journals (Sweden)

Lisha, V.

2017-01-01

Full Text Available modified crops which brought up a controversy on the safety usage of genetically modified organisms (GMOs. It has been implemented globally that all GMO products and its derived ingredients should have regulations on the usage and labelling. Thus, it is necessary to develop methods that allow rapid screening of GMO products to comply with the regulations. This study employed a reliable and flexible multiplex polymerase chain reaction (PCR method for the rapid detection of transgenic elements in genetically modified soy and maize along with the soybean LECTIN gene and maize ZEIN gene respectively. The selected four common transgenic elements were 35S promoter (35S; Agrobacterium tumefaciens nopaline synthase terminator (NOS; 5-enolypyruvylshikimate-3-phosphate synthase (epsps gene; and Cry1Ab delta-endotoxin (cry1Ab gene. Optimization of the multiplex PCR methods were carried out by using 1% Roundup ReadyTM Soybean (RRS as the certified reference material for soybean that produced fourplex PCR method detecting 35S promoter, NOS terminator, epsps gene and soybean LECTIN gene and by using 1% MON810 as the certified reference material for maize that produced triplex PCR method detecting 35S promoter, cry1Ab gene and maize ZEIN gene prior to screening of the GMO traits in various food products and animal feeds. 1/9 (11.1% of the animal feed contained maize and 1/15 (6.7% of the soybean food products showed positive results for the detection of GMO transgenic gene. None of the maize food products showed positive results for GMO transgenic gene. In total, approximately 4% of the food products and animal feed were positive as GMO. This indicated GMOs have not widely entered the food chain. However, it is necessary to have an appropriate screening method due to GMOs’ unknown potential risk to humans and to animals. This rapid screening method will provide leverage in terms of being economically wise, time saving and reliable.

Governing GMOs: The (Counter Movement for Mandatory and Voluntary Non-GMO Labels

Directory of Open Access Journals (Sweden)

Carmen Bain

2014-12-01

Full Text Available Since 2012 the anti-GMO (genetically modified organism movement has gained significant grassroots momentum in its efforts to require mandatory GMO food labels through state-level ballot and legislative efforts. Major food and agriculture corporations are opposed to mandatory GMO labels and have successfully defeated most of these initiatives. Nevertheless, these battles have garnered significant media attention and re-energized the debate over GMO crops and foods. In this paper, we argue that one of the most significant outcomes of this fight is efforts by food retailers and value-based food companies to implement voluntary non-GMO labels and brands. We draw on the governance and political consumerism literature to explore (counter movement efforts for mandatory labels and how these efforts are being institutionalized through private voluntary governance institutions. Our assessment is based on in-depth, semi-structured interviews with key informants from consumer and environmental organizations, agriculture and biotech companies, and government regulatory agencies, as well as a content analysis of food industry websites. A growing number of food retailers recognize the reputational and economic value that new niche markets for non-GMO foods can offer, while the anti-GMO movement views these efforts as a step in the direction of mandatory GMO labels. We conclude that voluntary labels may act to settle the labeling debate by mollifying agri-food industry concerns about mandatory labeling and meeting the desire of political consumers for greater choice and transparency but without addressing the broader social and environmental sustainability concerns that drives the anti-GMO movement in the first place.

Implementation of Electronic Transmission of Chemical Occurrence Data (CFP/EFSA/DATEX/2009/01) in Denmark

DEFF Research Database (Denmark)

Andersen, Jens Hinge

EFSA has prepared a standard data model for the transmission of chemical occurrence data and pesticide residues. This model is referred to as the “Standard Model” (SM) or the “Standard Sample Description” (SSD). The aim of the present project was to collect and transform data from the Danish...... national data repositories for chemical contaminants and pesticides and in coding these according to the SM and to facilitate the continued use of the SM in answering future EFSA data calls in these areas. Restructuring was performed using existing equipment and programming tools. SAS procedures and Excel...... mapping tables were developed to handle the data transformation. The main challenges were mapping existing data to the SM, synthesising necessary information missing in the national repository, and dealing with the complexity of pesticide substance reporting....

Analysis of GMO Plum Plant Culture in System Operations Failure

Science.gov (United States)

Mercado, Dianne

2017-01-01

GMO plum trees are being evaluated at the Kennedy Space Center as a possible candidate for future space crops. Previously conducted horticultural testing compared the performance of several plum genotypes in controlled environment chambers, resulting in a down-selection to the NASA-11 genotype. Precursory studies determined the water use requirements to sustain the plants as well as the feasibility of grafting non-GMO plum scions onto GMO plum rootstocks of NASA-5, NASA-10, and NASA-11 genotypes. This study follows the growth and horticultural progress of plum trees and in-vitro cultures from August 2017 to November 2017, and provides supplemental support for future GMO plum studies. The presence of Hurricane Irma in early September 2017 resulted in the plants undergoing material deterioration from major changes to their overall horticultural progress.

EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2015. Scientific Opinion on Flavouring Group Evaluation 213, Revision 2 (FGE.213Rev2): Consideration of genotoxic potential for α,β-unsaturated alicyclic ketones and precursors from chemical subgroup 2

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Nørby, Karin Kristiane

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF Panel) of the European Food Safety Authority (EFSA) was requested to evaluate the genotoxic potential of 26 flavouring substances from subgroup 2.7 of FGE.19 in Flavouring Group Evaluation (FGE) 213. In the first v...

Environmental risk assessment in GMO analysis.

Science.gov (United States)

Pirondini, Andrea; Marmiroli, Nelson

2010-01-01

Genetically modified or engineered organisms (GMOs, GEOs) are utilised in agriculture, expressing traits of interest, such as insect or herbicide resistance. Soybean, maize, cotton and oilseed rape are the GM crops with the largest acreage in the world. The distribution of GM acreage in the different countries is related with the different positions concerning labelling of GMO products: based on the principle of substantial equivalence, or rather based on the precautionary principle. The paper provides an overview on how the risks associated with release of GMO in the environments can be analysed and predicted, in view of a possible coexistence of GM and non-GM organisms in agriculture.Risk assessment procedures, both qualitative and quantitative, are compared in the context of application to GMOs considering also legislation requirements (Directive 2001/18/EC). Criteria and measurable properties to assess harm for human health and environmental safety are listed, and the possible consequences are evaluated in terms of significance.Finally, a mapping of the possible risks deriving from GMO release is reported, focusing on gene transfer to related species, horizontal gene transfer, direct and indirect effects on non target organisms, development of resistance in target organisms, and effects on biodiversity.

JRC GMO-Matrix: a web application to support Genetically Modified Organisms detection strategies.

Science.gov (United States)

Angers-Loustau, Alexandre; Petrillo, Mauro; Bonfini, Laura; Gatto, Francesco; Rosa, Sabrina; Patak, Alexandre; Kreysa, Joachim

2014-12-30

The polymerase chain reaction (PCR) is the current state of the art technique for DNA-based detection of Genetically Modified Organisms (GMOs). A typical control strategy starts by analyzing a sample for the presence of target sequences (GM-elements) known to be present in many GMOs. Positive findings from this "screening" are then confirmed with GM (event) specific test methods. A reliable knowledge of which GMOs are detected by combinations of GM-detection methods is thus crucial to minimize the verification efforts. In this article, we describe a novel platform that links the information of two unique databases built and maintained by the European Union Reference Laboratory for Genetically Modified Food and Feed (EU-RL GMFF) at the Joint Research Centre (JRC) of the European Commission, one containing the sequence information of known GM-events and the other validated PCR-based detection and identification methods. The new platform compiles in silico determinations of the detection of a wide range of GMOs by the available detection methods using existing scripts that simulate PCR amplification and, when present, probe binding. The correctness of the information has been verified by comparing the in silico conclusions to experimental results for a subset of forty-nine GM events and six methods. The JRC GMO-Matrix is unique for its reliance on DNA sequence data and its flexibility in integrating novel GMOs and new detection methods. Users can mine the database using a set of web interfaces that thus provide a valuable support to GMO control laboratories in planning and evaluating their GMO screening strategies. The platform is accessible at http://gmo-crl.jrc.ec.europa.eu/jrcgmomatrix/ .

Finding the joker among the maize endogenous reference genes for genetically modified organism (GMO) detection.

Science.gov (United States)

Paternò, Annalisa; Marchesi, Ugo; Gatto, Francesco; Verginelli, Daniela; Quarchioni, Cinzia; Fusco, Cristiana; Zepparoni, Alessia; Amaddeo, Demetrio; Ciabatti, Ilaria

2009-12-09

The comparison of five real-time polymerase chain reaction (PCR) methods targeted at maize ( Zea mays ) endogenous sequences is reported. PCR targets were the alcohol dehydrogenase (adh) gene for three methods and high-mobility group (hmg) gene for the other two. The five real-time PCR methods have been checked under repeatability conditions at several dilution levels on both pooled DNA template from several genetically modified (GM) maize certified reference materials (CRMs) and single CRM DNA extracts. Slopes and R(2) coefficients of all of the curves obtained from the adopted regression model were compared within the same method and among all of the five methods, and the limit of detection and limit of quantitation were analyzed for each PCR system. Furthermore, method equivalency was evaluated on the basis of the ability to estimate the target haploid genome copy number at each concentration level. Results indicated that, among the five methods tested, one of the hmg-targeted PCR systems can be considered equivalent to the others but shows the best regression parameters and a higher repeteability along the dilution range. Thereby, it is proposed as a valid module to be coupled to different event-specific real-time PCR for maize genetically modified organism (GMO) quantitation. The resulting practicability improvement on the analytical control of GMOs is discussed.

The role of a German multi-stakeholder standard for livestock products derived from non-GMO feed

NARCIS (Netherlands)

Venus, Thomas J.; Drabik, Dusan; Wesseler, Justus

2018-01-01

In Germany, products derived from livestock who were fed GMO are not required to be labeled as GMO. However, non-GMO labeling requires compliance with the national public non-GMO production standard, including a confirmation that no GM feed was used. In addition to the national standard, firms can

GMO Reignited in Science but Not in Law: A Flawed Framework Fuels France's Stalemate.

Science.gov (United States)

Robbins, Patricia B

2014-01-01

Following a statement released by a multitude of prominent scientists contesting the idea that there is a consensus on the safety of genetically modified organisms ("GMO"), this article addresses the European Union's ("EU") GMO regulatory framework, which has reluctantly permitted France to maintain an illegal ban on. MON8 10 for over a decade now. It notes that while the statement did nothing more than reignite the debate on GMO, much could and should be done to improve the framework to accommodate for the lack of true scientific understanding about the effects of GMO. This article identifies the specific areas of weakness in the EU GMO regulatory framework and recommends specific alterations. It concludes that although France's MON810 ban is illegal under existing law, the country's fears are neither unfounded nor unsupported and that the EU should work to alter its existing legal structure to parallel today's scientific uncertainty regarding GMO safety.

Genetically modified organisms (GMO in opinions completing secondary schools in Lublin

Directory of Open Access Journals (Sweden)

Lachowski Stanisław

2016-09-01

Full Text Available The objective of the conducted analysis is the opinion of adolescents completing secondary schools concerning genetically modified organisms (GMO and determination of the relationship between the level of knowledge concerning GMO, and evaluation of the safety of their use in industry and agriculture.

Nanopore sequencing technology: a new route for the fast detection of unauthorized GMO.

Science.gov (United States)

Fraiture, Marie-Alice; Saltykova, Assia; Hoffman, Stefan; Winand, Raf; Deforce, Dieter; Vanneste, Kevin; De Keersmaecker, Sigrid C J; Roosens, Nancy H C

2018-05-21

In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette. In the present study, we investigate the feasibility to integrate the described workflow with the MinION Next-Generation-Sequencing (NGS). The MinION sequencing platform can provide long read-lengths and deal with heterogenic DNA libraries, allowing for rapid and efficient delivery of sequences of interest. In addition, the ability of this NGS platform to characterize unauthorized and unknown GMO without any a priori knowledge has been assessed.

Dyrkning af GMO-afgrøder er ikke problemfrit

DEFF Research Database (Denmark)

Streibig, Jens Carl

2012-01-01

Middtvesten i USA oplever efter 16 år med GMO-afgrøder stigende problemer med resistent ukrudt - en udvikling der måske også kan ramme danske landmænd med de nye pesticidafgifter......Middtvesten i USA oplever efter 16 år med GMO-afgrøder stigende problemer med resistent ukrudt - en udvikling der måske også kan ramme danske landmænd med de nye pesticidafgifter...

Dietary Reference Values for choline

DEFF Research Database (Denmark)

Sjödin, Anders Mikael

2016-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derives Dietary Reference Values (DRVs) for choline. In this Opinion, the Panel considers dietary choline including choline compounds (e.g. glycerophosphocholine, phosphocholine...

Dietary reference values for riboflavin

DEFF Research Database (Denmark)

Sjödin, Anders Mikael

2017-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derives dietary reference values (DRVs) for riboflavin. The Panel considers that the inflection point in the urinary riboflavin excretion curve in relation to riboflavin intake...

A statistical approach to quantification of genetically modified organisms (GMO) using frequency distributions.

Science.gov (United States)

Gerdes, Lars; Busch, Ulrich; Pecoraro, Sven

2014-12-14

According to Regulation (EU) No 619/2011, trace amounts of non-authorised genetically modified organisms (GMO) in feed are tolerated within the EU if certain prerequisites are met. Tolerable traces must not exceed the so-called 'minimum required performance limit' (MRPL), which was defined according to the mentioned regulation to correspond to 0.1% mass fraction per ingredient. Therefore, not yet authorised GMO (and some GMO whose approvals have expired) have to be quantified at very low level following the qualitative detection in genomic DNA extracted from feed samples. As the results of quantitative analysis can imply severe legal and financial consequences for producers or distributors of feed, the quantification results need to be utterly reliable. We developed a statistical approach to investigate the experimental measurement variability within one 96-well PCR plate. This approach visualises the frequency distribution as zygosity-corrected relative content of genetically modified material resulting from different combinations of transgene and reference gene Cq values. One application of it is the simulation of the consequences of varying parameters on measurement results. Parameters could be for example replicate numbers or baseline and threshold settings, measurement results could be for example median (class) and relative standard deviation (RSD). All calculations can be done using the built-in functions of Excel without any need for programming. The developed Excel spreadsheets are available (see section 'Availability of supporting data' for details). In most cases, the combination of four PCR replicates for each of the two DNA isolations already resulted in a relative standard deviation of 15% or less. The aims of the study are scientifically based suggestions for minimisation of uncertainty of measurement especially in -but not limited to- the field of GMO quantification at low concentration levels. Four PCR replicates for each of the two DNA isolations

Dietary reference values for potassium

DEFF Research Database (Denmark)

Sjödin, Anders Mikael

2016-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derives dietary reference values (DRVs) for potassium. The Panel decides to set DRVs on the basis of the relationships between potassium intake and blood pressure and stroke...

Potential of cross-priming amplification and DNA-based lateral-flow strip biosensor for rapid on-site GMO screening.

Science.gov (United States)

Huang, Xin; Zhai, Congcong; You, Qimin; Chen, Hongjun

2014-07-01

The requirement to monitor the presence of genetically modified organisms (GMO) in a variety of marked products has generated an increasing demand for reliable, rapid, and time and cost-effective analytical methods. Here we report an on-site method for rapid detection of cauliflower mosaic virus promoter (CaMV 35S), a common element present in most GMO, using cross-priming amplification (CPA) technology. Detection was achieved using a DNA-based contamination-proof strip biosensor. The limit of detection was 30 copies for the pBI121 plasmid containing the CaMV 35S gene. The certified reference sample of GM maize line MON810 was detectable even at the low relative mass concentration of 0.05%. The developed CPA method had high specificity for the CaMV 35S gene, as compared with other GM lines not containing this gene and non-GM products. The method was further validated using nine real-world samples, and the results were confirmed by real-time PCR analysis. Because of its simplicity, rapidity, and high sensitivity, this method of detecting the CaMV 35S gene has great commercial prospects for rapid GMO screening of high-consumption food and agriculture products.

Possibilities of using the German Federal States' permanent soil monitoring program for the monitoring of potential effects of genetically modified organisms (GMO).

Science.gov (United States)

Toschki, Andreas; Jänsch, Stephan; Roß-Nickoll, Martina; Römbke, Jörg; Züghart, Wiebke

2015-01-01

In the Directive 2001/18/EC on the deliberate release of genetically modified organisms (GMO) into the environment, a monitoring of potential risks is prescribed after their deliberate release or placing on the market. Experience and data of already existing monitoring networks should be included. The present paper summarizes the major findings of a project funded by the Federal Agency for Nature Conservation (Nutzungsmöglichkeiten der Boden-Dauerbeobachtung der Länder für das Monitoring der Umweltwirkungen gentechnisch veränderter Pflanzen. BfN Skripten, Bonn-Bad Godesberg 369, 2014). The full report in german language can be accessed on http://www.bfn.de and is available as Additional file 1. The aim of the project was to check if it is possible to use the German permanent soil monitoring program (PSM) for the monitoring of GMO. Soil organism communities are highly diverse and relevant with respect to the sustainability of soil functions. They are exposed to GMO material directly by feeding or indirectly through food chain interactions. Other impacts are possible due to their close association to soil particles. The PSM program can be considered as representative with regard to different soil types and ecoregions in Germany, but not for all habitat types relevant for soil organisms. Nevertheless, it is suitable as a basic grid for monitoring the potential effects of GMO on soil invertebrates. PSM sites should be used to derive reference values, i.e. range of abundance and presence of different relevant species of soil organisms. Based on these references, it is possible to derive threshold values to define the limit of acceptable change or impact. Therefore, a minimum set of sites and minimum set of standardized methods are needed, i.e. characterization of each site, sampling of selected soil organism groups, adequate adaptation of methods for the purpose of monitoring of potential effects of GMO. Finally, and probably most demanding, it is needed to develop

How to deal with the upcoming challenges in GMO detection in food and feed.

Science.gov (United States)

Broeders, Sylvia R M; De Keersmaecker, Sigrid C J; Roosens, Nancy H C

2012-01-01

Biotech crops are the fastest adopted crop technology in the history of modern agriculture. The commercialisation of GMO is in many countries strictly regulated laying down the need for traceability and labelling. To comply with these legislations, detection methods are needed. To date, GM events have been developed by the introduction of a transgenic insert (i.e., promoter, coding sequence, terminator) into the plant genome and real-time PCR is the detection method of choice. However, new types of genetic elements will be used to construct new GMO and new crops will be transformed. Additionally, the presence of unauthorised GMO in food and feed samples might increase in the near future. To enable enforcement laboratories to continue detecting all GM events and to obtain an idea of the possible presence of unauthorised GMO in a food and feed sample, an intensive screening will become necessary. A pragmatic, cost-effective, and time-saving approach is presented here together with an overview of the evolution of the GMO and the upcoming needs.

How to Deal with the Upcoming Challenges in GMO Detection in Food and Feed

Directory of Open Access Journals (Sweden)

Sylvia R. M. Broeders

2012-01-01

Full Text Available Biotech crops are the fastest adopted crop technology in the history of modern agriculture. The commercialisation of GMO is in many countries strictly regulated laying down the need for traceability and labelling. To comply with these legislations, detection methods are needed. To date, GM events have been developed by the introduction of a transgenic insert (i.e., promoter, coding sequence, terminator into the plant genome and real-time PCR is the detection method of choice. However, new types of genetic elements will be used to construct new GMO and new crops will be transformed. Additionally, the presence of unauthorised GMO in food and feed samples might increase in the near future. To enable enforcement laboratories to continue detecting all GM events and to obtain an idea of the possible presence of unauthorised GMO in a food and feed sample, an intensive screening will become necessary. A pragmatic, cost-effective, and time-saving approach is presented here together with an overview of the evolution of the GMO and the upcoming needs.

Minister Tuiksoo : GMO väärib tõsist suhtumist ja laialdast arutelu

Index Scriptorium Estoniae

2006-01-01

Põllumajandusminister Ester Tuiksoo osales Viinis Euroopa Komisjoni ning EL-i eesistujariigi Austriaga koostöös korraldatud konverentsil "Geneetiliselt muundatud, tavapäraste ja mahepõllumajanduskultuuride kooseksisteerimine - valikuvabadus", kus arutati tootjatele ja tarbijatele valikuvabaduse tagamist toota GMO või GMO-vaba toodangut

NAIMA as a solution for future GMO diagnostics challenges.

Science.gov (United States)

Dobnik, David; Morisset, Dany; Gruden, Kristina

2010-03-01

In the field of genetically modified organism (GMO) diagnostics, real-time PCR has been the method of choice for target detection and quantification in most laboratories. Despite its numerous advantages, however, the lack of a true multiplexing option may render real-time PCR less practical in the face of future GMO detection challenges such as the multiplicity and increasing complexity of new transgenic events, as well as the repeated occurrence of unauthorized GMOs on the market. In this context, we recently reported the development of a novel multiplex quantitative DNA-based target amplification method, named NASBA implemented microarray analysis (NAIMA), which is suitable for sensitive, specific and quantitative detection of GMOs on a microarray. In this article, the performance of NAIMA is compared with that of real-time PCR, the focus being their performances in view of the upcoming challenge to detect/quantify an increasing number of possible GMOs at a sustainable cost and affordable staff effort. Finally, we present our conclusions concerning the applicability of NAIMA for future use in GMO diagnostics.

Etisk Råd: GMO kan bidrage med noget væsentligt

DEFF Research Database (Denmark)

Gjerris, Mickey; Birkler, Jacob

2014-01-01

Forslaget om at lade EU's medlemslande sætte kriterier for at godkende GMO'er til dyrkning rummer en mulighed for at løfte debatten ud over den ufrugtbare skin-diskussion om risiko.......Forslaget om at lade EU's medlemslande sætte kriterier for at godkende GMO'er til dyrkning rummer en mulighed for at løfte debatten ud over den ufrugtbare skin-diskussion om risiko....

EDF group - Reference Document 2005

International Nuclear Information System (INIS)

2006-01-01

The EDF Group is an integrated energy supplier operating in a wide range of electricity-related businesses: generation, transmission, distribution, sale and trading of energy. It is the main operator in the French electricity market and one of the leading electricity groups in Europe. With an installed capacity of 130.8 GW (123.9 GW in Europe), it contributes to the supply of energy and services to more than 40 million customers throughout the world (with approximately 36.7 million customers in Europe, more than 28 million of whom are in France). The EDF Group has built a business model balanced between deregulated and regulated operations in France and an international presence. In 2005, the Group recorded consolidated sales of euros 51,051 million, net income (Group share) of euros 3,242 million, and it achieved earnings before interests, taxes, depreciation and amortization of euros 13,010 million. This document is EDF Group's Reference Document for the year 2005. It contains information about: the Group activities, risk factors, Business Overview, Organizational Structure, Property, Plants and Equipment, Operating and Financial Review, Capital Resources, Research and Development, Patents and Licences, Trend Information, Financial Prospects, Administrative, Management, and Supervisory Bodies and Senior Management, Remuneration and Benefits, Board Practices, Employees/Human Resources, Major Shareholders, Related Party Transactions, Financial Information Concerning the Company's Assets and Liabilities, Financial Position and Profits and Losses, Material Contracts, Information on Holdings etc

Tiit Tammsaar GMO-toidu vastu / Sigrid Laev

Index Scriptorium Estoniae

Laev, Sigrid

2003-01-01

Põllumajandusminister Tiit Tammsaare sõnul ei suuda Eesti geneetiliselt muundatud toidule (GMO) tõenäoliselt vastu seista. EL-i riikide esindajad ei leidnud üksmeelt, kas lubada liitu uut tüüpi maisi Bt-11

New GMO regulations for old: Determining a new future for EU crop biotechnology

Science.gov (United States)

2017-01-01

ABSTRACT In this review, current EU GMO regulations are subjected to a point-by point analysis to determine their suitability for agriculture in modern Europe. Our analysis concerns present GMO regulations as well as suggestions for possible new regulations for genome editing and New Breeding Techniques (for which no regulations presently exist). Firstly, the present GMO regulations stem from the early days of recombinant DNA and are not adapted to current scientific understanding on this subject. Scientific understanding of GMOs has changed and these regulations are now, not only unfit for their original purpose, but, the purpose itself is now no longer scientifically valid. Indeed, they defy scientific, economic, and even common, sense. A major EU regulatory preconception is that GM crops are basically different from their parent crops. Thus, the EU regulations are “process based” regulations that discriminate against GMOs simply because they are GMOs. However current scientific evidence shows a blending of classical crops and their GMO counterparts with no clear demarcation line between them. Canada has a “product based” approach and determines the safety of each new crop variety independently of the process used to obtain it. We advise that the EC re-writes it outdated regulations and moves toward such a product based approach.  Secondly, over the last few years new genomic editing techniques (sometimes called New Breeding Techniques) have evolved. These techniques are basically mutagenesis techniques that can generate genomic diversity and have vast potential for crop improvement. They are not GMO based techniques (any more than mutagenesis is a GMO technique), since in many cases no new DNA is introduced. Thus they cannot simply be lumped together with GMOs (as many anti-GMO NGOs would prefer). The EU currently has no regulations to cover these new techniques. In this review, we make suggestions as to how these new gene edited crops may be regulated

New GMO regulations for old: Determining a new future for EU crop biotechnology.

Science.gov (United States)

Davison, John; Ammann, Klaus

2017-01-02

In this review, current EU GMO regulations are subjected to a point-by point analysis to determine their suitability for agriculture in modern Europe. Our analysis concerns present GMO regulations as well as suggestions for possible new regulations for genome editing and New Breeding Techniques (for which no regulations presently exist). Firstly, the present GMO regulations stem from the early days of recombinant DNA and are not adapted to current scientific understanding on this subject. Scientific understanding of GMOs has changed and these regulations are now, not only unfit for their original purpose, but, the purpose itself is now no longer scientifically valid. Indeed, they defy scientific, economic, and even common, sense. A major EU regulatory preconception is that GM crops are basically different from their parent crops. Thus, the EU regulations are "process based" regulations that discriminate against GMOs simply because they are GMOs. However current scientific evidence shows a blending of classical crops and their GMO counterparts with no clear demarcation line between them. Canada has a "product based" approach and determines the safety of each new crop variety independently of the process used to obtain it. We advise that the EC re-writes it outdated regulations and moves toward such a product based approach.  Secondly, over the last few years new genomic editing techniques (sometimes called New Breeding Techniques) have evolved. These techniques are basically mutagenesis techniques that can generate genomic diversity and have vast potential for crop improvement. They are not GMO based techniques (any more than mutagenesis is a GMO technique), since in many cases no new DNA is introduced. Thus they cannot simply be lumped together with GMOs (as many anti-GMO NGOs would prefer). The EU currently has no regulations to cover these new techniques. In this review, we make suggestions as to how these new gene edited crops may be regulated. The EU is at a

A high-throughput method for GMO multi-detection using a microfluidic dynamic array.

Science.gov (United States)

Brod, Fábio Cristiano Angonesi; van Dijk, Jeroen P; Voorhuijzen, Marleen M; Dinon, Andréia Zilio; Guimarães, Luis Henrique S; Scholtens, Ingrid M J; Arisi, Ana Carolina Maisonnave; Kok, Esther J

2014-02-01

The ever-increasing production of genetically modified crops generates a demand for high-throughput DNA-based methods for the enforcement of genetically modified organisms (GMO) labelling requirements. The application of standard real-time PCR will become increasingly costly with the growth of the number of GMOs that is potentially present in an individual sample. The present work presents the results of an innovative approach in genetically modified crops analysis by DNA based methods, which is the use of a microfluidic dynamic array as a high throughput multi-detection system. In order to evaluate the system, six test samples with an increasing degree of complexity were prepared, preamplified and subsequently analysed in the Fluidigm system. Twenty-eight assays targeting different DNA elements, GM events and species-specific reference genes were used in the experiment. The large majority of the assays tested presented expected results. The power of low level detection was assessed and elements present at concentrations as low as 0.06 % were successfully detected. The approach proposed in this work presents the Fluidigm system as a suitable and promising platform for GMO multi-detection.

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 4: suitability of taxonomic units notified to EFSA until March 2016

OpenAIRE

EFSA Panel on Biological Hazards (BIOHAZ); Allende, Ana; Bolton, Declan; Chemaly, Marianne; Davies, Robert; Fernandez Escamez, Pablo Salvador; Girones, Rosina; Herman, Lieve; Koutsoumanis, Konstantinos; Lindqvist, Roland; Nørrung, Birgit; Ricci, Antonia; Robertson, Lucy; Ru, Giuseppe; Sanaa, Moez

2016-01-01

EFSA was requested to assess the safety of a broad range of biological agents in the context of notifications for market authorisation as sources of food and feed additives, enzymes and plant protection products. The qualified presumption of safety (QPS) assessment was developed to provide a harmonised generic preassessment to support safety risk assessments performed by EFSA's scientific Panels. The safety of unambiguously defined biological agents (at the highest taxonomic unit appropriate ...

Development and validation of an integrated DNA walking strategy to detect GMO expressing cry genes.

Science.gov (United States)

Fraiture, Marie-Alice; Vandamme, Julie; Herman, Philippe; Roosens, Nancy H C

2018-06-27

Recently, an integrated DNA walking strategy has been proposed to prove the presence of GMO via the characterisation of sequences of interest, including their transgene flanking regions and the unnatural associations of elements in their transgenic cassettes. To this end, the p35S, tNOS and t35S pCAMBIA elements have been selected as key targets, allowing the coverage of most of GMO, EU authorized or not. In the present study, a bidirectional DNA walking method anchored on the CryAb/c genes is proposed with the aim to cover additional GMO and additional sequences of interest. The performance of the proposed bidirectional DNA walking method anchored on the CryAb/c genes has been evaluated in a first time for its feasibility using several GM events possessing these CryAb/c genes. Afterwards, its sensitivity has been investigated through low concentrations of targets (as low as 20 HGE). In addition, to illustrate its applicability, the entire workflow has been tested on a sample mimicking food/feed matrices analysed in GMO routine analysis. Given the successful assessment of its performance, the present bidirectional DNA walking method anchored on the CryAb/c genes can easily be implemented in GMO routine analysis by the enforcement laboratories and allows completing the entire DNA walking strategy in targeting an additional transgenic element frequently found in GMO.

Validation of the performance of a GMO multiplex screening assay based on microarray detection

NARCIS (Netherlands)

Leimanis, S.; Hamels, S.; Naze, F.; Mbongolo, G.; Sneyers, M.; Hochegger, R.; Broll, H.; Roth, L.; Dallmann, K.; Micsinai, A.; Dijk, van J.P.; Kok, E.J.

2008-01-01

A new screening method for the detection and identification of GMO, based on the use of multiplex PCR followed by microarray, has been developed and is presented. The technology is based on the identification of quite ubiquitous GMO genetic target elements first amplified by PCR, followed by direct

Scientific Opinion on dietary reference values for copper

DEFF Research Database (Denmark)

Sjödin, Anders Mikael

2015-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for copper. Owing to the absence of appropriate biomarkers of copper status and the limitations of available balance studies, the Panel...

GMO is dood, lang leve de gentechniek

NARCIS (Netherlands)

Arendonk, van J.A.M.

2004-01-01

Nu de consument met geen stok aan de gmo-voeding is te krijgen, bloeit de aandacht voor klassieke veredeling op. Dit traditionele proces plukt echter ook de vruchten van gentech. Er kan gerichter veredeld worden, met als resultaat tropisch fruit dat niet bederft tijdens de verscheping, of wellicht

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific Opinion on the risks to public health related to the presence of nickel in food and drinking water

OpenAIRE

Petersen, Annette

2015-01-01

EFSA received a request from the Hellenic Food Authority (EFET) for a scientific opinion on the risk to human health from the presence of nickel (Ni) in food, particularly in vegetables. The EFSA Panel on Contaminants in the Food Chain (CONTAM Panel) decided to extend the risk assessment also to drinking water. The reproductive and developmental toxicity in experimental animals was selected as the critical effect for the assessment of chronic effects of Ni. A tolerable daily intake of 2.8 µg ...

Increased efficacy for in-house validation of real-time PCR GMO detection methods.

Science.gov (United States)

Scholtens, I M J; Kok, E J; Hougs, L; Molenaar, B; Thissen, J T N M; van der Voet, H

2010-03-01

To improve the efficacy of the in-house validation of GMO detection methods (DNA isolation and real-time PCR, polymerase chain reaction), a study was performed to gain insight in the contribution of the different steps of the GMO detection method to the repeatability and in-house reproducibility. In the present study, 19 methods for (GM) soy, maize canola and potato were validated in-house of which 14 on the basis of an 8-day validation scheme using eight different samples and five on the basis of a more concise validation protocol. In this way, data was obtained with respect to the detection limit, accuracy and precision. Also, decision limits were calculated for declaring non-conformance (>0.9%) with 95% reliability. In order to estimate the contribution of the different steps in the GMO analysis to the total variation variance components were estimated using REML (residual maximum likelihood method). From these components, relative standard deviations for repeatability and reproducibility (RSD(r) and RSD(R)) were calculated. The results showed that not only the PCR reaction but also the factors 'DNA isolation' and 'PCR day' are important factors for the total variance and should therefore be included in the in-house validation. It is proposed to use a statistical model to estimate these factors from a large dataset of initial validations so that for similar GMO methods in the future, only the PCR step needs to be validated. The resulting data are discussed in the light of agreed European criteria for qualified GMO detection methods.

A high-throughput liquid bead array-based screening technology for Bt presence in GMO manipulation.

Science.gov (United States)

Fu, Wei; Wang, Huiyu; Wang, Chenguang; Mei, Lin; Lin, Xiangmei; Han, Xueqing; Zhu, Shuifang

2016-03-15

The number of species and planting areas of genetically modified organisms (GMOs) has been rapidly developed during the past ten years. For the purpose of GMO inspection, quarantine and manipulation, we have now devised a high-throughput Bt-based GMOs screening method based on the liquid bead array. This novel method is based on the direct competitive recognition between biotinylated antibodies and beads-coupled antigens, searching for Bt presence in samples if it contains Bt Cry1 Aa, Bt Cry1 Ab, Bt Cry1 Ac, Bt Cry1 Ah, Bt Cry1 B, Bt Cry1 C, Bt Cry1 F, Bt Cry2 A, Bt Cry3 or Bt Cry9 C. Our method has a wide GMO species coverage so that more than 90% of the whole commercialized GMO species can be identified throughout the world. Under our optimization, specificity, sensitivity, repeatability and availability validation, the method shows a high specificity and 10-50 ng/mL sensitivity of quantification. We then assessed more than 1800 samples in the field and food market to prove capacity of our method in performing a high throughput screening work for GMO manipulation. Our method offers an applicant platform for further inspection and research on GMO plants. Copyright © 2015 Elsevier B.V. All rights reserved.

GMO-afgrøder ikke den eneste løsning

DEFF Research Database (Denmark)

Jacobsen, Niels; Jacobsen, Sven-Erik; Sørensen, Marten

2010-01-01

Er GMO-afgrøder den optimale løsning på klodens fremtidige plantebaserede fødevareproduktion? Findes der alternativer? Det er der to meget klare svar på:GMOafgrøderer ikke den eneste løsning, og ja, der findes mange og langt mere attraktive, bæredygtige løsninger......Er GMO-afgrøder den optimale løsning på klodens fremtidige plantebaserede fødevareproduktion? Findes der alternativer? Det er der to meget klare svar på:GMOafgrøderer ikke den eneste løsning, og ja, der findes mange og langt mere attraktive, bæredygtige løsninger...

The structuring of GMO release and evaluation in EU law.

Science.gov (United States)

von Kries, Caroline; Winter, Gerd

2012-04-01

Genetically modified organisms (GMOs) and their behavior in the environment are complex and can only be assessed if the different components are distinguished. This article examines, how by EU law the real causation processes from the GMO release to various endpoints are dissected, individually analysed and then again viewed in their entirety. In addition, the articles includes, how the intellectual process of assessment is divided into the steps of tiered generation, shared submission and structured evaluation of relevant knowledge. The framework proposed for such an examination allows to identify strengths and weaknesses of GMO risk assessment in the EU. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Non-GMO genetically edited crop plants.

Science.gov (United States)

Kanchiswamy, Chidananda Nagamangala; Malnoy, Mickael; Velasco, Riccardo; Kim, Jin-Soo; Viola, Roberto

2015-09-01

Direct delivery of purified Cas9 protein with guide RNA into plant cells, as opposed to plasmid-mediated delivery, displays high efficiency and reduced off-target effects. Following regeneration from edited cells, the ensuing plant is also likely to bypass genetically modified organism (GMO) legislation as the genome editing complex is degraded in the recipient cells. Copyright © 2015 Elsevier Ltd. All rights reserved.

The in vitro sub-cellular localization and in vivo efficacy of novel chitosan/GMO nanostructures containing paclitaxel.

Science.gov (United States)

Trickler, W J; Nagvekar, A A; Dash, A K

2009-08-01

To determine the in vitro sub-cellular localization and in vivo efficacy of chitosan/GMO nanostructures containing paclitaxel (PTX) compared to a conventional PTX treatment (Taxol). The sub-cellular localization of coumarin-6 labeled chitosan/GMO nanostructures was determined by confocal microscopy in MDA-MB-231 cells. The antitumor efficacy was evaluated in two separate studies using FOX-Chase (CB17) SCID Female-Mice MDA-MB-231 xenograph model. Treatments consisted of intravenous Taxol or chitosan/GMO nanostructures with or without PTX, local intra-tumor bolus of Taxol or chitosan/GMO nanostructures with or without PTX. The tumor diameter and animal weight was monitored at various intervals. Histopathological changes were evaluated in end-point tumors. The tumor diameter increased at a constant rate for all the groups between days 7-14. After a single intratumoral bolus dose of chitosan/GMO containing PTX showed significant reduction in tumor diameter on day 15 when compared to control, placebo and intravenous PTX administration. The tumor diameter reached a maximal decrease (4-fold) by day 18, and the difference was reduced to approximately 2-fold by day 21. Qualitatively similar results were observed in a separate study containing PTX when administered intravenously. Chitosan/GMO nanostructures containing PTX are safe and effective administered locally or intravenously. Partially supported by DOD Award BC045664.

Scientific Opinion on the assessment of potential impacts of genetically modified plants on non-target organisms

DEFF Research Database (Denmark)

Arpaia, Salvatore; Bartsch, Detlef; Delos, Marc

The European Food Safety Authority (EFSA) asked the Panel on Genetically Modified Organisms to establish a self-tasking Working Group with the aim of (1) producing a scientific review of the current guidance of the GMO Panel for Environmental Risk Assessment (ERA), focusing on the potential impacts...... of GM plants on Non-Target Organisms (NTOs), (2) proposing criteria for NTOs selection, and (3) providing advise on standardized testing methodology. This initiative was undertaken in response to a need and request from a wide range of stakeholders, including the European Commission and Member States....... In first instance, the self-tasking Working Group on Non-Target Organisms (EFSA NTO WG) mainly considered impacts of GM plants on invertebrate species, but also took account of ecosystem functions that could be altered. The EFSA NTO WG considered the necessity for clear and objective protection goals...

Qualified Presumption of Safety (QPS) is a generic risk assessment approach applied by the European Food Safety Authority (EFSA)

DEFF Research Database (Denmark)

Leuschner, R. G. K.; Robinson, T. P.; Hugas, M.

2010-01-01

Qualified Presumption of Safety (QPS) is a generic risk assessment approach applied by the European Food Safety Authority (EFSA) to notified biological agents aiming at simplifying risk assessments across different scientific Panels and Units. The aim of this review is to outline the implementation...... and value of the QPS assessment for EFSA and to explain its principles such as the unambiguous identity of a taxonomic unit, the body of knowledge including potential safety concerns and how these considerations lead to a list of biological agents recommended for QPS which EFSA keeps updated through...

Tendency for interlaboratory precision in the GMO analysis method based on real-time PCR.

Science.gov (United States)

Kodama, Takashi; Kurosawa, Yasunori; Kitta, Kazumi; Naito, Shigehiro

2010-01-01

The Horwitz curve estimates interlaboratory precision as a function only of concentration, and is frequently used as a method performance criterion in food analysis with chemical methods. The quantitative biochemical methods based on real-time PCR require an analogous criterion to progressively promote method validation. We analyzed the tendency of precision using a simplex real-time PCR technique in 53 collaborative studies of seven genetically modified (GM) crops. Reproducibility standard deviation (SR) and repeatability standard deviation (Sr) of the genetically modified organism (GMO) amount (%) was more or less independent of GM crops (i.e., maize, soybean, cotton, oilseed rape, potato, sugar beet, and rice) and evaluation procedure steps. Some studies evaluated whole steps consisting of DNA extraction and PCR quantitation, whereas others focused only on the PCR quantitation step by using DNA extraction solutions. Therefore, SR and Sr for GMO amount (%) are functions only of concentration similar to the Horwitz curve. We proposed S(R) = 0.1971C 0.8685 and S(r) = 0.1478C 0.8424, where C is the GMO amount (%). We also proposed a method performance index in GMO quantitative methods that is analogous to the Horwitz Ratio.

A framework for a european network for a systematic environmental impact assessment of genetically modified organisms (GMO)

DEFF Research Database (Denmark)

Graef, Frieder; Römbke, Jörg; Binimelis, Rosa

2012-01-01

Network for systematic GMO impact assessment (ENSyGMO) with the aim directly to enhance ERA and post-market environmental monitoring (PMEM) of GM crops, to harmonize and ultimately secure the long-term socio-political impact of the ERA process and the PMEM in the EU. These goals would be achieved...... effects and use state-of-the art sampling, statistics and modelling approaches. To address public concerns and create confidence in the ENSyGMO results, actors with relevant specialist knowledge from various sectors should be involved....

Loop-Mediated Isothermal Amplification for Detection of Endogenous Sad1 Gene in Cotton: An Internal Control for Rapid Onsite GMO Testing.

Science.gov (United States)

Singh, Monika; Bhoge, Rajesh K; Randhawa, Gurinderjit

2018-04-20

Background : Confirming the integrity of seed samples in powdered form is important priorto conducting a genetically modified organism (GMO) test. Rapid onsite methods may provide a technological solution to check for genetically modified (GM) events at ports of entry. In India, Bt cotton is the commercialized GM crop with four approved GM events; however, 59 GM events have been approved globally. GMO screening is required to test for authorized GM events. The identity and amplifiability of test samples could be ensured first by employing endogenous genes as an internal control. Objective : A rapid onsite detection method was developed for an endogenous reference gene, stearoyl acyl carrier protein desaturase ( Sad1 ) of cotton, employing visual and real-time loop-mediated isothermal amplification (LAMP). Methods : The assays were performed at a constant temperature of 63°C for 30 min for visual LAMP and 62ºC for 40 min for real-time LAMP. Positive amplification was visualized as a change in color from orange to green on addition of SYBR ® Green or detected as real-time amplification curves. Results : Specificity of LAMP assays was confirmed using a set of 10 samples. LOD for visual LAMP was up to 0.1%, detecting 40 target copies, and for real-time LAMP up to 0.05%, detecting 20 target copies. Conclusions : The developed methods could be utilized to confirm the integrity of seed powder prior to conducting a GMO test for specific GM events of cotton. Highlights : LAMP assays for the endogenous Sad1 gene of cotton have been developed to be used as an internal control for onsite GMO testing in cotton.

Dietary reference values for thiamin

DEFF Research Database (Denmark)

Sjödin, Anders Mikael

2016-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derived dietary reference values (DRVs) for thiamin (vitamin B1). The Panel considers that data from depletion–repletion studies in adults on the amount of dietary thiamin intake...... were measured. Results from other depletion–repletion studies are in agreement with this value. The Panel agrees on the coefficient of variation of 20% used by the SCF to cover uncertainties related to distribution of thiamin requirements in the general population, and endorses the population reference...

The GMO sumrule and the πNN coupling constant

International Nuclear Information System (INIS)

Ericson, T.E.O.; Loiseau, B.; Thomas, A.W.

2000-01-01

The isovector GMO sumrule for forward πN scattering is critically evaluated using the precise π - p and π - d scattering lengths obtained recently from pionic atom measurements. The charged πNN coupling constant is then deduced with careful analysis of systematic and statistical sources of uncertainties. This determination gives directly from data g c 2 (GMO)/4π = 14.17±0.09 (statistic) ±0.17 (systematic) or f c 2 / 4π=0.078(11). This value is half-way between that of indirect methods (phase-shift analyses) and the direct evaluation from from backward np differential scattering cross sections (extrapolation to pion pole). From the π - p and π - d scattering lengths our analysis leads also to accurate values for (1/2)(a π - p +a π - n ) and (1/2) (a π - p -a π - n ). (orig.)

The GMO Sumrule and the πNN Coupling Constant

Science.gov (United States)

Ericson, T. E. O.; Loiseau, B.; Thomas, A. W.

The isovector GMO sumrule for forward πN scattering is critically evaluated using the precise π-p and π-d scattering lengths obtained recently from pionic atom measurements. The charged πNN coupling constant is then deduced with careful analysis of systematic and statistical sources of uncertainties. This determination gives directly from data gc2(GMO)/4π = 14.17±0.09 (statistic) ±0.17 (systematic) or fc2/ 4π=0.078(11). This value is half-way between that of indirect methods (phase-shift analyses) and the direct evaluation from from backward np differential scattering cross sections (extrapolation to pion pole). From the π-p and π-d scattering lengths our analysis leads also to accurate values for (1/2)(aπ-p+aπ-n) and (1/2) (aπ-p-aπ-n).

Safety Assessment of Food and Feed from GM Crops in Europe: Evaluating EFSA's Alternative Framework for the Rat 90-day Feeding Study.

Science.gov (United States)

Hong, Bonnie; Du, Yingzhou; Mukerji, Pushkor; Roper, Jason M; Appenzeller, Laura M

2017-07-12

Regulatory-compliant rodent subchronic feeding studies are compulsory regardless of a hypothesis to test, according to recent EU legislation for the safety assessment of whole food/feed produced from genetically modified (GM) crops containing a single genetic transformation event (European Union Commission Implementing Regulation No. 503/2013). The Implementing Regulation refers to guidelines set forth by the European Food Safety Authority (EFSA) for the design, conduct, and analysis of rodent subchronic feeding studies. The set of EFSA recommendations was rigorously applied to a 90-day feeding study in Sprague-Dawley rats. After study completion, the appropriateness and applicability of these recommendations were assessed using a battery of statistical analysis approaches including both retrospective and prospective statistical power analyses as well as variance-covariance decomposition. In the interest of animal welfare considerations, alternative experimental designs were investigated and evaluated in the context of informing the health risk assessment of food/feed from GM crops.

Průzkum poptávky po GMO a analýza faktorů ovlivňujících poptávku

OpenAIRE

Jankech, Aleš

2007-01-01

Tato práce se zabývá poptávkou po GMO. Přináší základní informace o GMO, vyjmenovává třídy GMO a především blíže rozebírá významné negativní a pozitivní vlivy, které působí na poptávku GMO. Práce obsahuje analýzu poptávky po GMO z dat získaných dotazníkovým šetřením.

Coverage of endangered species in environmental risk assessments at EFSA

NARCIS (Netherlands)

Brock, T.C.M.

2016-01-01

The EFSA performs environmental risk assessment (ERA) for single potential stressors such as plant protection products, genetically modified organisms and feed additives, and for invasive alien species that are harmful to plant health. This ERA focusses primarily on the use or spread of such

Statistical modelling of usual intake. Scientific report submitted to EFSA

NARCIS (Netherlands)

Voet, van der H.; Klaveren, van J.D.; Arcella, D.; Bakker, M.; Boeing, H.; Boon, P.E.; Crépét, A.; Dekkers, A.; Boer, de W.; Dodd, K.W.; Ferrari, P.; Goedhart, P.W.; Hart, A.; Heijden, van der G.W.A.M.; Kennedy, M.; Kipnis, V.; Knüppel, S.; Merten, C.; Ocké, M.; Slob, W.

2010-01-01

Within the EFSA Article 36 project “European Tool Usual Intake” (ETUI) a workshop was organised in May 2010 where the different available models to calculate usual intake were presented and discussed. This report integrates the workshop background document, the presentations given by experts, and

Development of a real-time PCR method for the differential detection and quantification of four solanaceae in GMO analysis: potato (Solanum tuberosum), tomato (Solanum lycopersicum), eggplant (Solanum melongena), and pepper (Capsicum annuum).

Science.gov (United States)

Chaouachi, Maher; El Malki, Redouane; Berard, Aurélie; Romaniuk, Marcel; Laval, Valérie; Brunel, Dominique; Bertheau, Yves

2008-03-26

The labeling of products containing genetically modified organisms (GMO) is linked to their quantification since a threshold for the presence of fortuitous GMOs in food has been established. This threshold is calculated from a combination of two absolute quantification values: one for the specific GMO target and the second for an endogenous reference gene specific to the taxon. Thus, the development of reliable methods to quantify GMOs using endogenous reference genes in complex matrixes such as food and feed is needed. Plant identification can be difficult in the case of closely related taxa, which moreover are subject to introgression events. Based on the homology of beta-fructosidase sequences obtained from public databases, two couples of consensus primers were designed for the detection, quantification, and differentiation of four Solanaceae: potato (Solanum tuberosum), tomato (Solanum lycopersicum), pepper (Capsicum annuum), and eggplant (Solanum melongena). Sequence variability was studied first using lines and cultivars (intraspecies sequence variability), then using taxa involved in gene introgressions, and finally, using taxonomically close taxa (interspecies sequence variability). This study allowed us to design four highly specific TaqMan-MGB probes. A duplex real time PCR assay was developed for simultaneous quantification of tomato and potato. For eggplant and pepper, only simplex real time PCR tests were developed. The results demonstrated the high specificity and sensitivity of the assays. We therefore conclude that beta-fructosidase can be used as an endogenous reference gene for GMO analysis.

Practicable group testing method to evaluate weight/weight GMO content in maize grains.

Science.gov (United States)

Mano, Junichi; Yanaka, Yuka; Ikezu, Yoko; Onishi, Mari; Futo, Satoshi; Minegishi, Yasutaka; Ninomiya, Kenji; Yotsuyanagi, Yuichi; Spiegelhalter, Frank; Akiyama, Hiroshi; Teshima, Reiko; Hino, Akihiro; Naito, Shigehiro; Koiwa, Tomohiro; Takabatake, Reona; Furui, Satoshi; Kitta, Kazumi

2011-07-13

Because of the increasing use of maize hybrids with genetically modified (GM) stacked events, the established and commonly used bulk sample methods for PCR quantification of GM maize in non-GM maize are prone to overestimate the GM organism (GMO) content, compared to the actual weight/weight percentage of GM maize in the grain sample. As an alternative method, we designed and assessed a group testing strategy in which the GMO content is statistically evaluated based on qualitative analyses of multiple small pools, consisting of 20 maize kernels each. This approach enables the GMO content evaluation on a weight/weight basis, irrespective of the presence of stacked-event kernels. To enhance the method's user-friendliness in routine application, we devised an easy-to-use PCR-based qualitative analytical method comprising a sample preparation step in which 20 maize kernels are ground in a lysis buffer and a subsequent PCR assay in which the lysate is directly used as a DNA template. This method was validated in a multilaboratory collaborative trial.

Rationalizing the GMO Debate: The Ordonomic Approach to Addressing Agricultural Myths.

Science.gov (United States)

Hielscher, Stefan; Pies, Ingo; Valentinov, Vladislav; Chatalova, Lioudmila

2016-05-09

The public discourse on the acceptability of genetically modified organisms (GMOs) is not only controversial, but also infused with highly emotional and moralizing rhetoric. Although the assessment of risks and benefits of GMOs must be a scientific exercise, many debates on this issue seem to remain impervious to scientific evidence. In many cases, the moral psychology attributes of the general public create incentives for both GMO opponents and proponents to pursue misleading public campaigns, which impede the comprehensive assessment of the full spectrum of the risks and benefits of GMOs. The ordonomic approach to economic ethics introduced in this research note is helpful for disentangling the socio-economic and moral components of the GMO debate by re- and deconstructing moral claims.

Coexistence of GMO production, labeling policies, and strategic firm interaction

NARCIS (Netherlands)

Venus, Thomas Johann

2017-01-01

This dissertation analyzes the market effects of the coexistence of genetically modified organism (GMO) and conventional production, labeling policies, and strategic firm interactions through vertical product differentiation. Although we focus on GMOs, the applied frameworks can be adopted and

GMO - pohled sociálních věd

Czech Academy of Sciences Publication Activity Database

Seidlová, Adéla

2003-01-01

Roč. 1, 1-2 (2003), s. 21-24 ISSN 1214-438X R&D Projects: GA AV ČR IBS7028205 Institutional research plan: CEZ:AV0Z7028912 Keywords : GMO * public opinion * risk society Subject RIV: AO - Sociology, Demography

Uninformed and disinformed society and the GMO market.

Science.gov (United States)

Twardowski, Tomasz; Małyska, Aleksandra

2015-01-01

The EU has a complicated regulatory framework, and this is slowing down the approval process of new genetically modified (GM) crops. Currently, labeling of GM organisms (GMOs) is mandatory in all Member States. However, the USA, in which GMO labeling is not mandatory, continues to lead the production of biotech crops, biopharmaceuticals, biomaterials, and bioenergy. Copyright © 2014 Elsevier Ltd. All rights reserved.

Identification of potentially hazardous human gene products in GMO risk assessment.

Science.gov (United States)

Bergmans, Hans; Logie, Colin; Van Maanen, Kees; Hermsen, Harm; Meredyth, Michelle; Van Der Vlugt, Cécile

2008-01-01

Genetically modified organisms (GMOs), e.g. viral vectors, could threaten the environment if by their release they spread hazardous gene products. Even in contained use, to prevent adverse consequences, viral vectors carrying genes from mammals or humans should be especially scrutinized as to whether gene products that they synthesize could be hazardous in their new context. Examples of such potentially hazardous gene products (PHGPs) are: protein toxins, products of dominant alleles that have a role in hereditary diseases, gene products and sequences involved in genome rearrangements, gene products involved in immunomodulation or with an endocrine function, gene products involved in apoptosis, activated proto-oncogenes. For contained use of a GMO that carries a construct encoding a PHGP, the precautionary principle dictates that safety measures should be applied on a "worst case" basis, until the risks of the specific case have been assessed. The potential hazard of cloned genes can be estimated before empirical data on the actual GMO become available. Preliminary data may be used to focus hazard identification and risk assessment. Both predictive and empirical data may also help to identify what further information is needed to assess the risk of the GMO. A two-step approach, whereby a PHGP is evaluated for its conceptual dangers, then checked by data bank searches, is delineated here.

Fabrication of novel GMO/Eudragit E100 nanostructures for enhancing oral bioavailability of carvedilol.

Science.gov (United States)

Patil, Sharvil S; Roy, Krishtey; Choudhary, Bhavana; Mahadik, Kakasaheb R

2016-08-01

In the present work, novel nanostructures comprising of glyceryl monooleate (GMO) and Eudragit E100 were prepared using high intensity ultrasonic homogenization. 3(2) Factorial design approach was used for optimization of nanostructures. Results of regression analysis revealed that the amount of GMO and Eudragit E100 had a drastic effect on particle size and percent entrapment efficiency. Optimized carvedilol-loaded nanostructures (Car-NS) were characterized by FTIR, TEM, DSC, in vitro drug release study. Pharmacokinetic parameters such as Cmax, Tmax, Ke, Ka, Vd and AUC were estimated for Car-NS upon its oral administration in Sprague-Dawley rats. Particle size of Car-NS was found to be 183 ± 2.43 nm with an entrapment efficiency of 81.4 ± 0.512%. FTIR studies revealed loading and chemical compatibility of carvedilol with the components of nanostructures. DSC thermograms did not show endothermic peak for melting of carvedilol which could be attributed to solubilization of carvedilol in molten GMO during DSC run. The prepared Car-NS released carvedilol in sustained manner over a period of 10 h as suggested by in vitro drug release study. The pharmacokinetic study of Car-NS showed significant improvement in Cmax (two fold, p GMO/Eudragit E100 nanostructures having ability to release the drug in sustained manner with enhanced oral bioavailability can prove to be a promising carrier system for poorly water soluble drugs.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014 . Scientific Opinion on the safety of vitamin D - enriched UV - treated baker‘s yeast

DEFF Research Database (Denmark)

Tetens, Inge; Poulsen, Morten

2014-01-01

, fine pastry and food supplements. The Panel considers that the provided compositional data, the specification, the data from batch testing, data on the stability on the production process are sufficient and do not give rise to safety concerns. The Panel concludes that the data provided are sufficient...... and do not give rise to safety concerns.The applicant intends to use the NFI as an alternative source of vitamin D for food supplements and for fortification of yeast-leavened bread, rolls and fine pastry at maximum concentrations of 5 μg vitamin D2 per 100 g of these foods. The applicant provided....../100 g bread, rolls and fine pastry, it is highly unlikely that Tolerable Upper Intake Levels as established by EFSA (EFSA NDA Panel, 2012) are exceeded. The Panel considers that UV-treated baker’s yeast exhibiting an enhanced content of vitamin D2 is safe under the intended conditions of use....

Food processor and retailer non-GMO standards in the US and EU and the driving role of regulations

NARCIS (Netherlands)

Castellari, Elena; Soregaroli, Claudio; Venus, Thomas J.; Wesseler, Justus

2018-01-01

In the last two decades, voluntary standards have played an increasing role in reshaping the non-GMO labeling schemes in the EU and the US. This work compares the mandatory and voluntary labeling schemes for food produced from or with GMO in these two markets. After reviewing the EU and US

[Role of the EFSA in risk management system regarding food contact materials and articles].

Science.gov (United States)

Cwiek-Ludwicka, Kazimiera; Półtorak, Hanna; Pawlicka, Marzena

2009-01-01

The role of the European Food Safety Authority (EFSA) in the risk management system regarding food contact materials and articles is related with the risk assessment of the substances for the European Commission. General rules for the authorisation of substances used in materials and articles intended to contact with food is established in the Regulation (EC) no 1935/2004. For the evaluation of substances their toxicological properties and magnitude of migration into food simulants is taken into account. Toxicological studies include the mutagenicity tests, oral toxicity studies, carcinogenicity, reproduction and also studies on absorption, distribution, metabolism and excretion of the substance and other studies when needed. The set of the relevant toxicological data for substance depends on the magnitude of migration. In the case of positive opinion by EFSA the substance appears on the Community list of authorised substances. Sometimes, the earlier evaluated and authorized substances must undergo re-evaluation due to their new toxicological properties or as a result of a presence in the food of their earlier unknown decomposition products. Examples of the selected substances which underwent re-evaluation by EFSA in the light of the current toxicological knowledge and the relevant activities undertaken by the European Commission have been presented.

Kommentarer til arbejdsdokument ENV/04/27. Dokument fra "Antibiotic resistance marker genes working group"

DEFF Research Database (Denmark)

Kjellsson, Gøsta

2012-01-01

"DMU har modtaget og vurderet det fremsendte dokument ENV/04/27 og bilag (mail fra Skov- og Naturstyrelsen d. 14-10-2004) om anvendelsen af antibiotikaresistensmarkører (ARM) hos GMO. Vi har tidligere flere gange kommenteret anvendelsen af antibiotikaresistens (se f.eks. brev til Skov- og Naturst...

Reference Projections Energy and Emissions 2005-2020

International Nuclear Information System (INIS)

Van Dril, A.W.N.; Elzenga, H.E.

2005-10-01

The Reference Projection 2005-2020 covers the future development of Dutch energy use, greenhouse gas emissions and air pollution up to 2020. The Reference projection is based on assumptions regarding economic, structural, technological and policy developments. Two scenarios have been used. The Strong Europe (SE) scenario is characterized by moderate economic growth and strong public responsibility. The Global Economy (GE) scenario assumes high economic growth and has a strong orientation towards private responsibility. Energy consumption continues to grow in both scenarios and energy intensity is declining in the GE-scenario. Gradual rise of temperature is now included in the estimates for space heating and air conditioning. Energy prices for end users will rise, due to increased imports of natural gas and rising costs of electricity generation. The share of renewables in electricity consumption increases considerably due to subsidies for wind at sea and biomass, up to the target of 9% in 2010. Emissions of non-CO2 greenhouse gases are reduced and stabilise after 2010. The Dutch Kyoto target is probably met in both scenarios, assuming considerable emission reduction ef-forts abroad. Acidifying emissions of NOx and SO2 stabilise after reductions, but at levels that exceed their national emission ceiling (NEC). Emissions of volatile organic compounds are projected to fall with approximately 25% between 2002 and 2010 below their NEC. Emissions of ammonia are projected to meet their NEC. The emission of particulate matter (PM10) will stabilise at present levels

M&R 2016, 154, (ClientEarth and Pesticide Action Network v EFSA)

NARCIS (Netherlands)

Peeters, M.G.W.M.

2015-01-01

Belangenafweging bij het bekendmaken van persoonsgegevens in het kader van een verzoek om milieu informatie die specifieke deskundigenadvisering betreft. In hoger beroep wordt het besluit van EFSA tot geheimhouden van de namen van externe deskundigen die geadviseerd hebben over de totstandkoming van

Reply to the EFSA (2016) on the relevance of recent publications (Hofmann et al. 2014, 2016) on environmental risk assessment and management of Bt-maize events (MON810, Bt11 and 1507).

Science.gov (United States)

Kruse-Plass, Maren; Hofmann, Frieder; Kuhn, Ulrike; Otto, Mathias; Schlechtriemen, Ulrich; Schröder, Boris; Vögel, Rudolf; Wosniok, Werner

2017-01-01

In this commentary, we respond to a report of the EFSA GMO Panel (EFSA EFSA Supp Publ, 1) that criticises the outcomes of two studies published in this journal (Hofmann et al. Environ Sci Eur 26: 24, 2; Environ Sci Eur 28: 14, 3). Both publications relate to the environmental risk assessment and management of Bt-maize, including maize events MON810, Bt11 and maize 1507. The results of Hofmann et al. (Environ Sci Eur 26: 24, 2), using standardised pollen mass filter deposition measurements, indicated that the EFSA Panel model had underestimated pollen deposition and, hence, exposure of non-target organisms to Bt-maize pollen. The results implied a need for safety buffer distances in the kilometre range for protected nature reserve areas instead of the 20-30 m range recommended by the EFSA Panel. As a result, the EFSA Panel revised their model (EFSA EFSA J 13: 4127, 4), adopting the slope of the empirical data from Hofmann et al. The intercept, however, was substantially reduced to less than 1% at one point by introducing further assumptions based on the estimates of mainly panel members, citing possible 'uncertainty'. Hofmann et al. (Environ Sci Eur 28: 14, 3) published extensive empirical data regarding pollen deposition on leaves. These results were part of a larger 3-year study involving detailed measurements of pollen release, dispersal and deposition over the maize flowering period. The data collected in situ confirmed the previous predictions of Hofmann et al. (Environ Sci Eur 26: 24, 2). Mean levels and observed variability of pollen deposition on maize and four lepidopteran host plants exceeded the assumptions and disagreed with the conclusions of the EFSA Panel. The EFSA Panel reacted in a report (EFSA EFSA Supp Publ, 1) criticising the methods and outcomes of the two published studies of Hofmann et al. while reaffirming their original recommendations. We respond here point-by-point, showing that the critique is not justified. Based on our results on

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 6: suitability of taxonomic units notified to EFSA until March 2017

NARCIS (Netherlands)

Ricci, Antonia; Allende, Ana; Bolton, Declan; Chemaly, Marianne; Davies, Robert; Girones, Rosina; Koutsoumanis, Kostas; Lindqvist, Roland; Nørrung, Birgit; Robertson, Lucy; Ru, Giuseppe; Fernandez Escamez, Pablo Salvador; Sanaa, Moez; Simmons, Marion; Skandamis, Panagiotis; Snary, Emma; Speybroeck, Niko; Kuile, Ter Benno; Threlfall, John; Wahlström, Helene; Cocconcelli, Pier Sandro; Peixe, Luisa; Maradona, Miguel Prieto; Querol, Amparo; Suarez, Juan Evaristo; Sundh, Ingvar; Vlak, Just; Correia, Sandra; Herman, Lieve

2017-01-01

The qualified presumption of safety (QPS) concept was developed to provide a harmonised generic pre-evaluation to support safety risk assessments of biological agents performed by EFSA's scientific Panels. The identity, body of knowledge, safety concerns and antimicrobial resistance of valid

Expressional regulation of CKI1 provides molecular evidence for hormone interplay during Arabidopsis root development

Czech Academy of Sciences Publication Activity Database

Hejátko, Jan; Borkovcová, Petra; Pernisová, M.; Souček, Přemysl; Šišková, H.; Dobešová, R.; Palme, K.; Brzobohatý, Břetislav

2005-01-01

Roč. 409, - (2005), S27 [2nd International Symposium Auxins and Cytokinins in Plant Development. 07.07.2005-12.07.2005, Prague] R&D Projects: GA MŠk(CZ) LN00A081; GA AV ČR(CZ) IAA600380507 Institutional research plan: CEZ:AV0Z50040507 Keywords : CKI1 gene * cytokinin * auxin Subject RIV: BO - Biophysics

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific Opinion on acrylamide in food

DEFF Research Database (Denmark)

Petersen, Annette

EFSA was asked to deliver a scientific opinion on acrylamide (AA) in food. AA has widespread uses as an industrial chemical. It is also formed when certain foods are prepared at temperatures above 120 °C and low moisture, especially in foods containing asparagine and reducing sugars. The CONTAM...... Panel evaluated 43 419 analytical results from food commodities. AA was found at the highest levels in solid coffee substitutes and coffee, and in potato fried products. Mean and 95th percentile dietary AA exposures across surveys and age groups were estimated at 0.4 to 1.9 µg/kg body weight (b.w.) per...

Proceedings of the 1st International Operational Modal Analysis Conference, April 26-27, 2005, Copenhagen, Denmark

DEFF Research Database (Denmark)

These proceedings contain approximately 100 papers presented at the 1st International Operational Modal Conference, Copenhagen, Denmark, April 26 - 27, 2005. The conference is jointly organised by Structural Vibration Systems (SVS), Aalborg, Brüel & Kjær Sound and Vibration Measurement A/S and th......These proceedings contain approximately 100 papers presented at the 1st International Operational Modal Conference, Copenhagen, Denmark, April 26 - 27, 2005. The conference is jointly organised by Structural Vibration Systems (SVS), Aalborg, Brüel & Kjær Sound and Vibration Measurement A....../S and the Department of Building Technology and Structural Engineering at Aalborg University. It is the first conference ever to focus solely on experimental modal analysis for cases where only the responses are measured. It was decided to hold the conference to generate interest in operational modal analysis (also...... effort and higher versatility. However, because the mathematical background was initially weak, computer power was insufficient and the equipment not sensitive enough for this kind of testing, the technology has only become popular during the last 3 - 4 years. For this reason, the first conference...

Ühiskondliku Leppe Sihtasutus tahab GMO üle otsustamise anda elanikele / Ain Lember

Index Scriptorium Estoniae

Lember, Ain

2005-01-01

Ühiskondliku Leppe Sihtasutus peab mõttekaks ühineda üle-euroopalise algatusega muuta EL seadusandlust selliselt, et GMO-vabade piirkondade kehtestamiseks õigus jääb maaomanike ja ametnike asemel elanikele

Cell microarrays on photochemically modified polytetrafluoroethylene

Czech Academy of Sciences Publication Activity Database

Mikulíková, R.; Moritz, S.; Gumpenberger, T.; Olbrich, M.; Romanin, C.; Bačáková, Lucie; Švorčík, V.; Heitz, J.

2005-01-01

Roč. 26, č. 27 (2005), s. 5572-5580 ISSN 0142-9612 R&D Projects: GA AV ČR(CZ) IAA5011301 Grant - others:CZ-AT(CZ) Scientific-Technical Cooperation 2004-9; Jubiläumsfonds der Österreichischen Nationalbank(AT) 10549 Institutional research plan: CEZ:AV0Z50110509 Keywords : polytetrafluoroethylene * surface modification * endothelial cells Subject RIV: EI - Biotechnology ; Bionics Impact factor: 4.698, year: 2005

Knowledge of adolescents completing secondary schools concerning genetically modified organisms (GMO)

OpenAIRE

Florek-Łuszczki Magdalena; Lachowski Stanisław; Chmielewski Jarosław; Jurkiewicz Anna

2016-01-01

The objective of the conducted analyses is the evaluation of the level of knowledge concerning the scope of problems related with genetically modified organism (GMO) amongst adolescents completing secondary schools and the determination of the relationship between the level of this knowledge and the selected demographic traits of the adolescents examined.

Assessment of recent developmental immunotoxicity studies with bisphenol A in the context of the 2015 EFSA t-TDI

Science.gov (United States)

Hessel, Ellen V.S.; Ezendam, Janine; van Broekhuizen, Fleur A.; Hakkert, Betty; DeWitt, Jamie; Granum, Berit; Guzylack, Laurence; Lawrence, B. Paige; Penninks, Andre; Rooney, Andrew A.; Piersma, Aldert H.; van Loveren, Henk

2017-01-01

Summary Humans are exposed to bisphenol A (BPA) mainly through the diet, air, dust, skin contact and water. There are concerns about adverse health effects in humans due to exposure to bisphenol A (BPA). The European Food Safety Authority (EFSA) has extensively reviewed the available literature to establish a temporary Tolerable Daily Intake (t-TDI). This exposure level was based on all available literature published before the end of 2012. Since then, new experimental animal studies have emerged, including those that identified effects of BPA on the immune system after developmental exposure. These studies indicate that developmental immunotoxicity might occur at lower dose levels than previously observed and on which the current EFSA t-TDI is based. The Dutch National Institute for Public Health and the Environment (RIVM) organized an expert workshop in September 2015 to consider recently published studies on the developmental immunotoxicity of bisphenol A (BPA). Key studies were discussed in the context of other experimental studies. The workshop concluded that these new experimental studies provide credible evidence for adverse immune effects after developmental exposure to BPA at 5 μg/kg BW/day from gestation day 15 to postnatal day 21. Supportive evidence for adverse immune effects in similar dose ranges was obtained from other publications that were discussed during the workshop. The dose level associated with adverse immune effects is considerably lower than the dose used by EFSA for deriving the t-TDI. The workshop unanimously concluded that the current EFSA t-TDI warrants reconsideration in the context of all currently available data. PMID:27352639

Reference outlook for energy and emissions 2005-2020

International Nuclear Information System (INIS)

Van Dril, A.W.N.; Elzenga, H.E.

2005-05-01

The Reference Projection 2005-2020 covers the future development of Dutch energy use, greenhouse gas emissions and air pollution up to 2020. The Reference projection is based on assumptions regarding economic, structural, technological and policy developments. Two scenarios have been used. The Strong Europe (SE) scenario is characterized by moderate economic growth and strong public responsibility. The Global Economy (GE) scenario assumes high economic growth and has a strong orientation towards private responsibility. Energy consumption continues to grow in both scenarios and energy intensity is declining in the GE-scenario. Gradual rise of temperature is now included in the estimates for space heating and air conditioning. Energy prices for end users will rise, due to increased imports of natural gas and rising costs of electricity generation. The share of renewables in electricity consumption increases considerably due to subsidies for wind at sea and biomass, up to the target of 9% in 2010. Emissions of non-CO2 greenhouse gases are reduced and stabilise after 2010. The Dutch Kyoto target is probably met in both scenarios, assuming considerable emission reduction efforts abroad. Acidifying emissions of NOx and SO2 stabilise after reductions, but at levels that exceed their national emission ceiling (NEC). Emissions of volatile organic compounds are projected to fall with approximately 25% between 2002 and 2010 below their NEC. Emissions of ammonia are projected to meet their NEC. The emission of fine particles (PM10) will stabilise at present levels [nl

Can systematic reviews inform GMO risk assessment and risk management?

Directory of Open Access Journals (Sweden)

Christian eKohl

2015-08-01

Full Text Available Systematic reviews represent powerful tools to identify, collect, synthesize and evaluate primary research data on specific research questions in a highly standardized and reproducible manner. They enable the defensible synthesis of outcomes by increasing precision and minimizing bias whilst ensuring transparency of the methods used. This makes them especially valuable to inform evidence-based risk analysis and decision making in various topics and research disciplines. Although seen as a gold standard for synthesizing primary research data, systematic reviews are not without limitations as they are often cost, labor and time intensive and the utility of synthesis outcomes depends upon the availability of sufficient and robust primary research data. In this paper we 1 consider the added value systematic reviews could provide when synthesizing primary research data on genetically modified organisms (GMO and 2 critically assess the adequacy and feasibility of systematic review for collating and analyzing data on potential impacts of GMOs in order to better inform specific steps within GMO risk assessment and risk management. The regulatory framework of the EU is used as an example, although the issues we discuss are likely to be more widely applicable.

Can Systematic Reviews Inform GMO Risk Assessment and Risk Management?

Science.gov (United States)

Kohl, Christian; Frampton, Geoff; Sweet, Jeremy; Spök, Armin; Haddaway, Neal Robert; Wilhelm, Ralf; Unger, Stefan; Schiemann, Joachim

2015-01-01

Systematic reviews represent powerful tools to identify, collect, synthesize, and evaluate primary research data on specific research questions in a highly standardized and reproducible manner. They enable the defensible synthesis of outcomes by increasing precision and minimizing bias whilst ensuring transparency of the methods used. This makes them especially valuable to inform evidence-based risk analysis and decision making in various topics and research disciplines. Although seen as a "gold standard" for synthesizing primary research data, systematic reviews are not without limitations as they are often cost, labor and time intensive and the utility of synthesis outcomes depends upon the availability of sufficient and robust primary research data. In this paper, we (1) consider the added value systematic reviews could provide when synthesizing primary research data on genetically modified organisms (GMO) and (2) critically assess the adequacy and feasibility of systematic review for collating and analyzing data on potential impacts of GMOs in order to better inform specific steps within GMO risk assessment and risk management. The regulatory framework of the EU is used as an example, although the issues we discuss are likely to be more widely applicable.

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific Opinion on the risks to public health related to the presence of nickel in food and drinking water

DEFF Research Database (Denmark)

Petersen, Annette

EFSA received a request from the Hellenic Food Authority (EFET) for a scientific opinion on the risk to human health from the presence of nickel (Ni) in food, particularly in vegetables. The EFSA Panel on Contaminants in the Food Chain (CONTAM Panel) decided to extend the risk assessment also...... to drinking water. The reproductive and developmental toxicity in experimental animals was selected as the critical effect for the assessment of chronic effects of Ni. A tolerable daily intake of 2.8 µg Ni/kg body weight (b.w.) per day was derived from a lower 95 % confidence limit for a benchmark dose at 10...... after oral exposure to Ni was selected as the critical effect suitable for the assessment of acute effects of Ni. A lowest BMDL10 of 1.1 µg Ni/kg b.w. was derived for the incidence of SCD following oral exposure to Ni of human volunteers. The CONTAM Panel applied a margin of exposure (MOE) approach...

Inclusion and Implementation of Socio-Economic Considerations in GMO Regulations: Needs and Recommendations

Directory of Open Access Journals (Sweden)

Rosa Binimelis

2016-01-01

Full Text Available Socio-economic considerations are included in the regulatory frameworks on genetically modified organisms (GMOs of many countries. This is a reflection of an increasing interest in and recognition of the necessity to consider a broader range of issues when conducting a GMO risk assessment. At the same time, there are discussions about how socio-economic considerations can be identified and how their assessment can be carried out. To provide an understanding of the advances achieved so far, we describe the state of the art of existing biosafety institutional frameworks, legislation and policies with provisions on socio-economic considerations. We analyse the scope of the socio-economic considerations that have been included, the methodological options taken and the role of participatory processes and stakeholders involvement in the GMO-related decision-making. Since many of the countries that have legislation for assessing socio-economic considerations lack implementation experience, we provide an analysis of how implementation has evolved in Norway with the intention to illustrate that the inclusion of socio-economic considerations might be based on a learning process. Norway was the first country to include broader issues in its GMO assessment process, and is at present one of the countries with the most experience on implementation of these issues. Finally, we emphasise that there is a great need for training on how to perform assessments of socio-economic considerations, as well as reflection on possible ways for inclusion of participatory processes.

Knowledge of adolescents completing secondary schools concerning genetically modified organisms (GMO

Directory of Open Access Journals (Sweden)

Florek-Łuszczki Magdalena

2016-06-01

Full Text Available The objective of the conducted analyses is the evaluation of the level of knowledge concerning the scope of problems related with genetically modified organism (GMO amongst adolescents completing secondary schools and the determination of the relationship between the level of this knowledge and the selected demographic traits of the adolescents examined.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on Dihydrocapsiate

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on the safety of a synthetic dihydrocapsiate (DHC) as a food ingredient in the context of Regulation (EC) No 258/97 taking into account...

Pilot project on the Danish implementation of FoodEx2 as part of the Standard Sample Description for the electronic transmission of harmonised chemical occurrence data to EFSA (NP/EFSA/DCM/2012/01)

DEFF Research Database (Denmark)

Andersen, Jens Hinge; Christensen, Tue

The National Food Institute at the Technical University of Denmark has been recoding and transmitting data for chemical contaminants and pesticide residues to EFSA in the SSD format on behalf of the Danish Veterinary and Food Authority using FoodEx1 and MATRIX food classification. To prepare...... for the updated data format for food description and classification, FoodEx2, this terminology has been fully translated into the Danish language. The translation tables (based on FoodEx1 as food classification) prepared while performing the data transmission grant project (Electronic Transmission of Chemical...... Occurrence Data (CFP/EFSA/DATEX/2009/01)) have been supplemented with the FoodEx2 code, comprising of base term and additional (non-implicit) facets. Approximately 1000 distinct food descriptions from the LIMS have been associated with the equivalent FoodEx2 codes. The performance of FoodEx2 in classifying...

Risk regulation and deliberation in EU administrative governance: GMO regulation and its reform

NARCIS (Netherlands)

Weimer, M.

2015-01-01

The article analyses the problems of EU risk regulation of genetically modified organisms (GMOs) through the lens of deliberative theories of EU law and governance, such as deliberative supranationalism and experimentalist governance. Previous research had suggested that the GMO issue is not

A high-throughput method for GMO multi-detection using a microfluidic dynamic array

NARCIS (Netherlands)

Brod, F.C.A.; Dijk, van J.P.; Voorhuijzen, M.M.; Dinon, A.Z.; Guimarães, L.H.S.; Scholtens, I.M.J.; Arisi, A.C.M.; Kok, E.J.

2014-01-01

The ever-increasing production of genetically modified crops generates a demand for high-throughput DNAbased methods for the enforcement of genetically modified organisms (GMO) labelling requirements. The application of standard real-time PCR will become increasingly costly with the growth of the

The GMO case in France: politics, lawlessness and postmodernism.

Science.gov (United States)

Kuntz, Marcel

2014-07-03

The GMO debacle in France is analyzed in the light of the balance of forces around this controversy, the changes in position of governments and the opponents' strategic use of intimidation. These factors have caused insurmountable difficulties for scientific experimentations and assessment of the technology, as well as for farmers attempting to grow GM maize in this country. The change from a "modern" to a "postmodern" framing of official public debates and scientific institutions has not appeased confrontations concerning GMOs.

ANALISIS ARGUMENTASI MAHASISWA PENDIDIKAN BIOLOGI PADA ISU SOSIOSAINFIK KONSUMSI GENETICALLY MODIFIED ORGANISM (GMO

Directory of Open Access Journals (Sweden)

Y. Herlanti

2014-04-01

Full Text Available Penelitian ini bertujuan untuk menganalisis argumentasi yang dikemukakan oleh mahasiswa pendidikan biologi terkait isu sosiosaintifik yaitu konsumsi pangan Genetically Modified Organism (GMO.  Penelitian menggunakan metode survei secara online.  Partisipan yang berasal dari semester III-VII Universitas Islam Negeri Jakarta yang secara sukarela mengisi kuisioner online yang diunggah pada weblog. Hasil penelitian menunjukkan isu sosiosaintifik GMO lebih banyak ditanggapi secara saintifik oleh partisipan.  Argumentasi sebagian besar berada pada level II, yaitu telah mampu mengungkapkan sebuah klaim disertai dengan alasan. Hanya sedikit yang sudah mampu memberikan argumen secara holistik (level IV, yaitu mampu mengungkapkan argumen dengan alasan yang kuat yang tidak mudah dibantah.  Umumnya argumentasi yang dikemukan partisipan berjenis argumentasi sederhana dan argumentasi tipe rantai.  Berdasarkan temuan ini, perlu dikembangkan sebuah model perkuliahan yang dapat meningkatkan keterampilan berargumentasi. This research aimed to analyze the argument for socioscientifik issue “Genetically Modified Organism (GMO Food Consumtion”.  This reseach used online survey.  Participant filled online questionaire that uploaded in weblog.  Participants are student of biology education in Jakarta Islamic State University. The result showed most participants gave scientific view in their argument.  Most of argumentations were in level II; participants gave a klaim within a warrant.  Only a few argument were in level IV, it’s a holistic argument that contained a klaim, a warrant, a backing, and a rebuttal.  Most of argument had simple type or chain type.  From this result, university must develop strategies of lecturing to improve argumentation skill.

Ultrafiltered pig leukocyte extract (UPLE, IMUNOR) potentiates hematopoiesis-stimulating effects of G-CSF in vitro and improves the outcome of treatment of hematopoietic radiation damage in mice with G-CSF

Czech Academy of Sciences Publication Activity Database

Vacek, Antonín; Hofer, Michal; Schneiderová, H.; Svoboda, J.

2005-01-01

Roč. 27, č. 4 (2005), s. 647-659 ISSN 0892-3973 R&D Projects: GA AV ČR(CZ) IBS5004009; GA AV ČR(CZ) KSK5011112 Institutional research plan: CEZ:AV0Z50040507 Keywords : ultrafiltered pig leukocyte extract * Imunor * granulocyte colony-stimulating factor Subject RIV: BO - Biophysics Impact factor: 0.557, year: 2005

Technical Report, Outcome of the public consultation on the draft Scientific Opinion of the EFSA Panel on Contaminants on the Food Chain (CONTAM) on acrylamide in food

DEFF Research Database (Denmark)

Petersen, Annette

The EFSA Panel on Contaminants in the Food Chain (CONTAM Panel) has endorsed its draft Scientific Opinion on the risks to public health related to the presence of acrylamide (AA) in food. The opinion has undergone a public consultation from 1 July 2014 to 15 September 2014. Overall, EFSA has...

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on Dietary Reference Values for fats, including saturated fatty acids, polyunsaturated fatty acids, monounsaturated fatty acids, trans fatty acids, and cholesterol

DEFF Research Database (Denmark)

Tetens, Inge

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) deals with the setting of Dietary Reference Values (DRVs) for fats. A lower bound of the reference intake range for total fat of 20 energy % (E%) and an upper bound of 35 E% are proposed. Fat intake in infants can......-linolenic acid (ALA) of 0.5 E%; not to set an UL for ALA; to set an AI of 250 mg for eicosapentaenoic acid (EPA) plus docosahexaenoic acid (DHA) for adults; to set an AI of 100 mg DHA for infants (>6 months) and young children...... gradually be reduced from 40 E% in the 6-12 month period to 35-40 E% in the 2nd and 3rd year of life. For specific fatty acids the following is proposed: saturated fatty acid (SFA) and trans fatty acid intake should be as low as possible; not to set any DRV for cis-monounsaturated fatty acids......; not to formulate a DRV for the intake of total cis-polyunsaturated fatty acids (PUFA); not to set specific values for the n-3/n-6 ratio; to set an Adequate Intake (AI) of 4 E% for linoleic acid (LA); not to set any DRV for arachidonic acid; not to set an UL for total or any of the n-6 PUFA; to set an AI for alpha...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on bovine lactoferrin

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to carry out the additional assessment for ‘lactoferrin’ as a food ingredient in the context of Regulation (EC) No 258/97 taking into account the comments and objections...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on bovine lactoferrin

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to carry out the additional assessment of ‘lactoferrin’ as a food ingredient in the context of Regulation (EC) No 258/97 taking into account the comments and objections...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to “non-digestible oligo- and polysaccharides including galactooligosaccharides, oligofructose, polyfructose and inulin” and “increase in calcium

DEFF Research Database (Denmark)

Tetens, Inge

to deliver an opinion on the scientific substantiation of a health claim related to “non-digestible oligo- and polysaccharides including galacto-oligosaccharides, oligofructose, polyfructose and inulin” and “increase in calcium absorption”. The scope of the application was proposed to fall under a health...... claim referring to children’s development and health. The food constituents that are proposed by the applicant to be the subject of the health claim are “non-digestible oligo- and polysaccharides including galacto-oligosaccharides, oligofructose, polyfructose and inulin”. Upon requests by EFSA...... for clarification on the food constituent, the applicant did not clarify the food constituent that is the subject of the health claim. The Panel considers that the food constituents, “non-digestible oligo- and polysaccharides including galacto-oligosaccharides, oligofructose, polyfructose and inulin”, which...

Multiplex enrichment quantitative PCR (ME-qPCR): a high-throughput, highly sensitive detection method for GMO identification.

Science.gov (United States)

Fu, Wei; Zhu, Pengyu; Wei, Shuang; Zhixin, Du; Wang, Chenguang; Wu, Xiyang; Li, Feiwu; Zhu, Shuifang

2017-04-01

Among all of the high-throughput detection methods, PCR-based methodologies are regarded as the most cost-efficient and feasible methodologies compared with the next-generation sequencing or ChIP-based methods. However, the PCR-based methods can only achieve multiplex detection up to 15-plex due to limitations imposed by the multiplex primer interactions. The detection throughput cannot meet the demands of high-throughput detection, such as SNP or gene expression analysis. Therefore, in our study, we have developed a new high-throughput PCR-based detection method, multiplex enrichment quantitative PCR (ME-qPCR), which is a combination of qPCR and nested PCR. The GMO content detection results in our study showed that ME-qPCR could achieve high-throughput detection up to 26-plex. Compared to the original qPCR, the Ct values of ME-qPCR were lower for the same group, which showed that ME-qPCR sensitivity is higher than the original qPCR. The absolute limit of detection for ME-qPCR could achieve levels as low as a single copy of the plant genome. Moreover, the specificity results showed that no cross-amplification occurred for irrelevant GMO events. After evaluation of all of the parameters, a practical evaluation was performed with different foods. The more stable amplification results, compared to qPCR, showed that ME-qPCR was suitable for GMO detection in foods. In conclusion, ME-qPCR achieved sensitive, high-throughput GMO detection in complex substrates, such as crops or food samples. In the future, ME-qPCR-based GMO content identification may positively impact SNP analysis or multiplex gene expression of food or agricultural samples. Graphical abstract For the first-step amplification, four primers (A, B, C, and D) have been added into the reaction volume. In this manner, four kinds of amplicons have been generated. All of these four amplicons could be regarded as the target of second-step PCR. For the second-step amplification, three parallels have been taken for

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific Opinion on the safety of caffeine

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies was asked to deliver a scientific opinion on the safety of caffeine, providing advice on caffeine intakes, from all dietary sources that do not give rise to concerns about adverse health...... not give rise to safety concerns. The same amount does not give rise to safety concerns when consumed .../L of caffeine, taurine and d-glucurono-γ-lactone, respectively), as well as alcohol at doses up to about 0.65 g/kg bw, would not affect the safety of single doses of caffeine up to 200 mg. Habitual caffeine consumption up to 400 mg per day does not give rise to safety concerns for non-pregnant adults. Habitual...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to fruits and/or vegetables (ID 1212, 1213, 1214, 1217, 1218, 1219, 1301, 1425, 1426, 1427, 1428, 1429, 1430) and to the “Mediterranean diet” (ID 1423) pursuant

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to fruits and/or vegetables and to the “Mediterranean diet”. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...

Destruction of public and governmental experiments of GMO in Europe.

Science.gov (United States)

Kuntz, Marcel

2012-01-01

The purpose of this article is to compile the destruction of GMO trials from academic or governmental research institutes in Europe, in a factual manner and to highlight their main characteristics. About 80 acts of vandalism against academic or governmental research on GMOs are identified, mainly in 4 countries; namely France, Germany, the United Kingdom and Switzerland. Examples are also provided for Italy and Belgium. The general conclusions that can be drawn from these acts are also discussed.

The application of transcriptomics in the comparative safety assessment of (GMO-derived) plant products

NARCIS (Netherlands)

Kok, E.J.

2008-01-01

National and international organizations have discussed current approaches to the safety assessment of complex (plant) food products in general and the safety assessment of GMO-derived food products in particular. One of the recommendations of different expert meetings was that the new analytical

Role of the European Food Safety Authority (EFSA in providing scientific advice on the welfare of food producing animals

Directory of Open Access Journals (Sweden)

Jordi Serratosa

2010-01-01

Full Text Available The survey describes the work of the Animal Health and Welfare (AHAW Panel of the European Food Safety Authority (EFSA in the provision of scientific advice on the welfare of food producing animals including animal health and food safety aspects, where relevant, and on the impact of these scientific assessments on the EU regulatory framework. EFSA was created in 2002 with the mission to provide advice and scientific and technical support for the Community legislation and policies in all fields which have a direct or indirect impact on food and feed safety, plant health, environment and animal health and animal welfare. When providing objective and independent science-based advice, the risk assessment approach should be followed, whenever possible. The AHAW Panel of EFSA provides specific advices on risk factors related to animal diseases and welfare, mainly of food producing animals, including fish. According to EFSA’s remit, ethical, socio-economic, cultural and religious aspects are outside the scope of the EFSA’s assessments. Since 2004, the Animal Health and Welfare Panel of EFSA adopted a total of 21 scientific opinions on animal welfare. Animal diseases and food safety aspects have also been taken into account, where relevant. Animal welfare aspects have been considered in some scientific opinions on animal diseases (e.g. AI, FMD. The AHAW Panel is currently working on five scientific opinions on the welfare of dairy cows and on the welfare aspects of the stunning and killing of farmed fish for eight fish species (salmon, trout, carp, eel, tuna, sea bass, sea bream and turbot. The possible interactions and implications for food safety and animal disease have been considered, when relevant, in most of the AW scientific opinions, involving other areas of expertise in EFSA, like Biohazards, Contaminants and Plant Health. The final aim of EFSA’s scientific assessments on animal welfare is to support animal welfare EU legislation on the

Fuzzy-logic based strategy for validation of multiplex methods: example with qualitative GMO assays.

Science.gov (United States)

Bellocchi, Gianni; Bertholet, Vincent; Hamels, Sandrine; Moens, W; Remacle, José; Van den Eede, Guy

2010-02-01

This paper illustrates the advantages that a fuzzy-based aggregation method could bring into the validation of a multiplex method for GMO detection (DualChip GMO kit, Eppendorf). Guidelines for validation of chemical, bio-chemical, pharmaceutical and genetic methods have been developed and ad hoc validation statistics are available and routinely used, for in-house and inter-laboratory testing, and decision-making. Fuzzy logic allows summarising the information obtained by independent validation statistics into one synthetic indicator of overall method performance. The microarray technology, introduced for simultaneous identification of multiple GMOs, poses specific validation issues (patterns of performance for a variety of GMOs at different concentrations). A fuzzy-based indicator for overall evaluation is illustrated in this paper, and applied to validation data for different genetically modified elements. Remarks were drawn on the analytical results. The fuzzy-logic based rules were shown to be applicable to improve interpretation of results and facilitate overall evaluation of the multiplex method.

EFSA ; Scientific Opinion on Flavouring Group Evaluation 98 (FGE.98): Consideration of three ring-unsaturated delta-lactones)

DEFF Research Database (Denmark)

Larsen, John Christian; Nørby, Karin Kristiane; Beltoft, Vibe Meister

evaluation is necessary, as laid down in Commission Regulation (EC) No 1565/2000. The present consideration concerns a group of three unsaturated delta-lactones [FL-no: 10.031, 10.037 and 10.044] previously evaluated by the JECFA at their 49th meeting in 1997. The JECFA considered that further information...... on the metabolism of these three substances was required and that they should be evaluated together with other substances containing alpha,beta-unsaturation and that, therefore, their evaluation should be deferred. However, the EFSA Panel has considered that these three JECFA evaluated aliphatic lactones can...... be hydrolysed and metabolised to innocuous products in line with the aliphatic lactones evaluated by EFSA in FGE.10Rev2. The substances were evaluated through a stepwise approach that integrates information on structure-activity relationships, intake from current uses, toxicological threshold of concern...

Abelovu cenu v roce 2005 získal Peter Lax

Czech Academy of Sciences Publication Activity Database

Křížek, Michal

2005-01-01

Roč. 50, č. 4 (2005), s. 265-269 ISSN 0032-2423 R&D Projects: GA MŠk(CZ) 1P05ME749 Institutional research plan: CEZ:AV0Z10190503 Keywords : Lax-Milgram lemma * Lax-Wendroff theorem * Korteweg-de Vries equation Subject RIV: BA - General Mathematics

Development and applicability of a ready-to-use PCR system for GMO screening.

Science.gov (United States)

Rosa, Sabrina F; Gatto, Francesco; Angers-Loustau, Alexandre; Petrillo, Mauro; Kreysa, Joachim; Querci, Maddalena

2016-06-15

With the growing number of GMOs introduced to the market, testing laboratories have seen their workload increase significantly. Ready-to-use multi-target PCR-based detection systems, such as pre-spotted plates (PSP), reduce analysis time while increasing capacity. This paper describes the development and applicability to GMO testing of a screening strategy involving a PSP and its associated web-based Decision Support System. The screening PSP was developed to detect all GMOs authorized in the EU in one single PCR experiment, through the combination of 16 validated assays. The screening strategy was successfully challenged in a wide inter-laboratory study on real-life food/feed samples. The positive outcome of this study could result in the adoption of a PSP screening strategy across the EU; a step that would increase harmonization and quality of GMO testing in the EU. Furthermore, this system could represent a model for other official control areas where high-throughput DNA-based detection systems are needed. Copyright © 2016 The Authors. Published by Elsevier Ltd.. All rights reserved.

Dietary reference values for vitamin K

DEFF Research Database (Denmark)

Sjödin, Anders Mikael

2017-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) derives dietary reference values (DRVs) for vitamin K. In this Opinion, the Panel considers vitamin K to comprise both phylloquinone and menaquinones. The Panel considers that none...... of the biomarkers of vitamin K intake or status is suitable by itself to derive DRVs for vitamin K. Several health outcomes possibly associated with vitamin K intake were also considered but data could not be used to establish DRVs. The Panel considers that average requirements and population reference intakes...... for vitamin K cannot be derived for adults, infants and children, and therefore sets adequate intakes (AIs). The Panel considers that available evidence on occurrence, absorption, function and content in the body or organs of menaquinones is insufficient, and, therefore, sets AIs for phylloquinone only...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014 . Scientific Opinion on the substantiation of a health claim related to zinc and normal growth pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

Inter-laboratory analysis of selected genetically modified plant reference materials with digital PCR.

Science.gov (United States)

Dobnik, David; Demšar, Tina; Huber, Ingrid; Gerdes, Lars; Broeders, Sylvia; Roosens, Nancy; Debode, Frederic; Berben, Gilbert; Žel, Jana

2018-01-01

Digital PCR (dPCR), as a new technology in the field of genetically modified (GM) organism (GMO) testing, enables determination of absolute target copy numbers. The purpose of our study was to test the transferability of methods designed for quantitative PCR (qPCR) to dPCR and to carry out an inter-laboratory comparison of the performance of two different dPCR platforms when determining the absolute GM copy numbers and GM copy number ratio in reference materials certified for GM content in mass fraction. Overall results in terms of measured GM% were within acceptable variation limits for both tested dPCR systems. However, the determined absolute copy numbers for individual genes or events showed higher variability between laboratories in one third of the cases, most possibly due to variability in the technical work, droplet size variability, and analysis of the raw data. GMO quantification with dPCR and qPCR was comparable. As methods originally designed for qPCR performed well in dPCR systems, already validated qPCR assays can most generally be used for dPCR technology with the purpose of GMO detection. Graphical abstract The output of three different PCR-based platforms was assessed in an inter-laboratory comparison.

Worldwide Emerging Environmental Issues Affecting the U.S. Military. Summarizing Environmental Security Monthly Scanning May 2005 - May 2006

Science.gov (United States)

2006-05-01

adverse ecotoxicological effects. [March 2006. Military Implications; Sources] Dechlorane Plus® Detected in Atmosphere An atmospheric monitoring...China, genetically modified rice illegal cultivation and commercialization is spreading, increasing concerns that the non-approved variety could...restrictions on cultivation , some also refer to regulations on import and use in food and feed. As the EU countries do not have a common GMO policy

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific opinion on niacin and contribution to normal energy-yielding metabolism: evaluation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific opinion on biotin and contribution to normal energy-yielding metabolism: evaluation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

Highly Sensitive GMO Detection Using Real-Time PCR with a Large Amount of DNA Template: Single-Laboratory Validation.

Science.gov (United States)

Mano, Junichi; Hatano, Shuko; Nagatomi, Yasuaki; Futo, Satoshi; Takabatake, Reona; Kitta, Kazumi

2018-03-01

Current genetically modified organism (GMO) detection methods allow for sensitive detection. However, a further increase in sensitivity will enable more efficient testing for large grain samples and reliable testing for processed foods. In this study, we investigated real-time PCR-based GMO detection methods using a large amount of DNA template. We selected target sequences that are commonly introduced into many kinds of GM crops, i.e., 35S promoter and nopaline synthase (NOS) terminator. This makes the newly developed method applicable to a wide range of GMOs, including some unauthorized ones. The estimated LOD of the new method was 0.005% of GM maize events; to the best of our knowledge, this method is the most sensitive among the GM maize detection methods for which the LOD was evaluated in terms of GMO content. A 10-fold increase in the DNA amount as compared with the amount used under common testing conditions gave an approximately 10-fold reduction in the LOD without PCR inhibition. Our method is applicable to various analytical samples, including processed foods. The use of other primers and fluorescence probes would permit highly sensitive detection of various recombinant DNA sequences besides the 35S promoter and NOS terminator.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to glycaemic carbohydrates and maintenance of normal brain function (ID 603, 653) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

; attention; memory”. The target population is assumed to be the general population. In the context of the proposed wordings, the Panel assumes that the claimed effects refer to the maintenance of normal brain function. The Panel considers that maintenance of normal brain function is a beneficial...... claims in relation to glycaemic carbohydrates and maintenance of normal brain function. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to caffeine and increased alertness pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from SmithKline Beecham Limited, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of United Kingdom, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to choline and “development of brain” pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

European Food Safety Authority; Response to comments on the Scientific Opinion of the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) on the scientific substantiation of a health claim related to beta-palmitate and increased calcium absorption pursuant to Article 14 of Regulation (EC

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, EFSA was asked to review the scientific comments received on the Scientific Opinion of the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) on the scientific substantiation of a health claim related to beta-palmitate and increased...... calcium absorption pursuant to Article 14 of Regulation (EC) No 1924/2006. Comments submitted to EFSA via the European Commission Services originated from the applicant (IDACE). EFSA has reviewed the comments and shared them with the chair of the NDA Panel, Prof. Albert Flynn, and the chair of the NDA...

EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA); Scientific Opinion on establishing Food-Based Dietary Guidelines

DEFF Research Database (Denmark)

Tetens, Inge

This Opinion of the EFSA Panel on Dietetic Products, Nutrition, and Allergies (NDA) provides guidance on the translation of nutrient based dietary advice into guidance, intended for the European population as a whole, on the contribution of different foods or food groups to an overall diet...

EFSA Panel on Dietetic Products, Nutrition and Allergies; Scientific Opinion on the substantiation of health claims related to creatine and increased attention (ID 1524) and improvement of memory (ID 1528) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to creatine and increased attention and improvement of memory. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...... stakeholders. The food constituent that is the subject of the health claims is creatine. The Panel considers that creatine is sufficiently characterised....

Development and application of a multi-targeting reference plasmid as calibrator for analysis of five genetically modified soybean events.

Science.gov (United States)

Pi, Liqun; Li, Xiang; Cao, Yiwei; Wang, Canhua; Pan, Liangwen; Yang, Litao

2015-04-01

Reference materials are important in accurate analysis of genetically modified organism (GMO) contents in food/feeds, and development of novel reference plasmid is a new trend in the research of GMO reference materials. Herein, we constructed a novel multi-targeting plasmid, pSOY, which contained seven event-specific sequences of five GM soybeans (MON89788-5', A2704-12-3', A5547-127-3', DP356043-5', DP305423-3', A2704-12-5', and A5547-127-5') and sequence of soybean endogenous reference gene Lectin. We evaluated the specificity, limit of detection and quantification, and applicability of pSOY in both qualitative and quantitative PCR analyses. The limit of detection (LOD) was as low as 20 copies in qualitative PCR, and the limit of quantification (LOQ) in quantitative PCR was 10 copies. In quantitative real-time PCR analysis, the PCR efficiencies of all event-specific and Lectin assays were higher than 90%, and the squared regression coefficients (R(2)) were more than 0.999. The quantification bias varied from 0.21% to 19.29%, and the relative standard deviations were from 1.08% to 9.84% in simulated samples analysis. All the results demonstrated that the developed multi-targeting plasmid, pSOY, was a credible substitute of matrix reference materials, and could be used as a reliable reference calibrator in the identification and quantification of multiple GM soybean events.

EFSA Panel on Plant Protection Products and their Residues (PPR); EFSA Scientific Opinion on the science behind the revision of the guidance document on dermal absorption

DEFF Research Database (Denmark)

Petersen, Annette

will be finalised and published only after adoption and publication of this opinion. This opinion has been developed after a public consultation of EFSA on the current guidance document and an outsourced project carried out by the UK Chemicals Regulation Directorate (CRD, 2010). It is not intended...... that have an impact on absorption, a description of important elements in the design of experimental studies and an analysis of available data on dermal absorption of PPPs. The PPR Panel concludes that assessment of dermal absorption in the absence of specific studies can be performed based on default...

Dissolved inorganic carbon, pH, alkalinity, temperature, salinity and other variables collected from discrete sample and profile observations using CTD, Coulometer for DIC measurement and other instruments from the MIRAI in the North Pacific Ocean from 2005-09-13 to 2005-10-27 (NODC Accession 0112265)

Data.gov (United States)

National Oceanic and Atmospheric Administration, Department of Commerce — NODC Accession 0112265 includes chemical, discrete sample, physical and profile data collected from MIRAI in the North Pacific Ocean from 2005-09-13 to 2005-10-27...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for zinc

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values for zinc, using a two-stage factorial approach and reference values for body weight. The first stage of estimating physiological requirements used studies...... from 2.4 to 11.8 mg/day. PRIs for infants and children were derived by assuming a coefficient of variation of 10 %, and range from 2.9 to 14.2 mg/day. For pregnancy and lactation, additional zinc requirements related to fetal and maternal tissues and transfer of zinc into breast milk, respectively...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to iodine and contribution to normal cognitive development pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to iodine and contribution to normal thyroid function pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to “complex carbohydrates” and “contribute to satiety” pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to prunes and contribution to normal bowel function pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...

ERK inhibition sensitizes CZ415-induced anti-osteosarcoma activity in vitro and in vivo.

Science.gov (United States)

Yin, Gang; Fan, Jin; Zhou, Wei; Ding, Qingfeng; Zhang, Jun; Wu, Xuan; Tang, Pengyu; Zhou, Hao; Wan, Bowen; Yin, Guoyong

2017-10-10

mTOR is a valuable oncotarget for osteosarcoma. The anti-osteosarcoma activity by a novel mTOR kinase inhibitor, CZ415, was evaluated. We demonstrated that CZ415 potently inhibited survival and proliferation of known osteosarcoma cell lines (U2OS, MG-63 and SaOs2), and primary human osteosarcoma cells. Further, CZ415 provoked apoptosis and disrupted cell cycle progression in osteosarcoma cells. CZ415 treatment in osteosarcoma cells concurrently blocked mTORC1 and mTORC2 activation. Intriguingly, ERK-MAPK activation could be a major resistance factor of CZ415. ERK inhibition (by MEK162/U0126) or knockdown (by targeted ERK1/2 shRNAs) dramatically sensitized CZ415-induced osteosarcoma cell apoptosis. In vivo , CZ415 oral administration efficiently inhibited U2OS tumor growth in mice. Its activity was further potentiated with co-administration of MEK162. Collectively, we demonstrate that ERK inhibition sensitizes CZ415-induced anti-osteosarcoma activity in vitro and in vivo . CZ415 could be further tested as a promising anti-osteosarcoma agent, alone or in combination of ERK inhibition.

Preparation of Emulsifying Wax/GMO Nanoparticles and Evaluation as a Delivery System for Repurposing Simvastatin in Bone Regeneration.

Science.gov (United States)

Eskinazi-Budge, Aaron; Manickavasagam, Dharani; Czech, Tori; Novak, Kimberly; Kunzler, James; Oyewumi, Moses O

2018-05-30

Simvastatin (Sim) is a widely known drug in the treatment of hyperlipidemia that has attracted so much attention in bone regeneration based on its potential osteoanabolic effect. However, repurposing of Sim in bone regeneration will require suitable delivery systems that can negate undesirable off-target/side effects. In this study, we have investigated a new lipid nanoparticle (NP) platform that was fabricated using a binary blend of emulsifying wax (Ewax) and glyceryl monooleate (GMO). Using the binary matrix materials, NPs loaded with Sim (0-500 µg/mL) were prepared and showed an average particle size of about 150 nm. NP size stability was dependent on Sim concentration loaded in NPs. The suitability of NPs prepared with the binary matrix materials in Sim delivery for potential application in bone regeneration was supported by biocompatibility in pre-osteoclastic and pre-osteoblastic cells. Additional data demonstrated that biofunctional Sim was released from NPs that facilitated differentiation of osteoblasts (cells that form bones) while inhibiting differentiation of osteoclasts (cells that resorb bones). The overall work demonstrated the preparation of NPs from Ewax/GMO blends and characterization to ascertain potential suitability in Sim delivery for bone regeneration. Additional studies on osteoblast and osteoclast functions are warranted to fully evaluate the efficacy simvastatin-loaded Ewax/GMO NPs using in-vitro and in-vivo approaches.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to DHA and contribution to normal brain development pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from DSM Nutritional Products, submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of the United Kingdom, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver...

At the end of the day everything boils down to politics: the evolving of German policy toward GMO crops and the existing stagnation.

Science.gov (United States)

Katzek, Jens

2014-07-03

Today it is "en vogue" to oppose the use of GMO plants not only in the environmental- and consumer-protection movement, the Green and the Social Democratic Party in Germany, but also in the conservative parties of the political spectrum. This article describes how such an atmosphere was able to develop over the last twenty years. An atmosphere in which almost everyone in favor of GMO plants within these parties is now quiet-because the political price of supporting the technology would be simply too high.

Pracující matky a genderové role ve výsledcích mezinárodního longitudinálního výzkumu

Czech Academy of Sciences Publication Activity Database

Hašková, Hana

2005-01-01

Roč. 6, č. 1 (2005), s. 22-27 ISSN 1213-0028 R&D Projects: GA AV ČR(CZ) KJB7028402 Institutional research plan: CEZ:AV0Z70280505 Keywords : gender roles * international comparison * work and family balance Subject RIV: AO - Sociology, Demography www. gender online.cz

Comparison of beverage consumption in adult populations from three different countries: do the international reference values allow establishing the adequacy of water and beverage intakes?

Science.gov (United States)

Nissensohn, Mariela; Fuentes Lugo, Daniel; Serra-Majem, Lluis

2016-07-13

Recommendations of adequate total water intake (aTWI) have been proposed by the European Food Safety Agency (EFSA) and the Institute of Medicine (IOM)of the United States of America. However, there are differences in the approach used to support them: IOM recommendation is based on average intakes observed in NHANES III (Third National Health and Nutrition Examination Survey) and EFSA recommendation on a combination of observed intakes from 13 different European countries. Despite these recommendations of aTWI, the currently available scientifi c evidence is not sufficient to establish a cut-off value that would prevent disease, reduce the risk for chronic diseases or improve health status. To compare the average daily consumption of fluids (water and other beverages) in selective samples of population from Mexico, US and Spain, evaluating the quantity of fluid intake and understanding the contribution of each fluid type to the total fl uid intake. We also aim to determine if they reached adequate intake (AI) values, as defi ned by three different criteria: IOM, EFSA and water density. Three studies were compared: from Mexico, the National Health and Nutrition Survey conducted in 2012 (NHNS 2012); from US, the NHANES III 2005-2010 and from Spain the ANIBES study leaded in 2013. Different categories of beverages were used to establish the pattern of energy intake for each country. Only adult population was selected. TWI of each study was compared with EFSA and IOM AI recommendations, as well as applying the criterion of water density (mL/kcal). The American study obtained the higher value of total kcal/day from food and beverages (2,437 ± 13). Furthermore, the percentage of daily energy intake coming from beverages was, for American adults, 21%. Mexico was slightly behind with 19% and Spain ANIBES study registered only 12%. ANIBES showed signifi cantly low AI values for the overall population, but even more alarming in the case of males. Only 12% of men, in

EFSA Panel on Biological Hazards (BIOHAZ); Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2011 update)

DEFF Research Database (Denmark)

Hald, Tine

) assessment was developed by EFSA for its own use to provide a generic risk assessment approach applicable across EFSA’s scientific Panels, for biological agents notified for intentional use in the whole food chain. The safety of unambiguously defined biological agents at the highest taxonomic unit......EFSA is requested to assess the safety of a broad range of biological agents (including microorganisms and viruses) in the context of notifications for market authorisation as sources of food and feed additives, enzymes and plant protection products. The qualified presumption of safety (QPS...

Risk identification in food safety: Strategy and outcomes of the EFSA emerging risks exchange network (EREN), 2010-2014

DEFF Research Database (Denmark)

Costa, M. C.; Goumperis, T.; Andersson, W.

2017-01-01

should be considered emerging or not, and if it merited further consideration, such as generating data on the issue, starting a full risk assessment and/or consultation of other bodies. According to the emerging risks identification process set in place at EFSA, the issues discussed and found...

Marketingová strategie VOYO.cz

OpenAIRE

Novotná, Markéta

2012-01-01

The aim of the diploma thesis is to analyse and evaluate the marketing strategy of VOYO.cz and recommend improvements of the current strategy. Theoretical part is divided into three chapters. The first chapter deals with the basic concept of marketing and the definition of marketing process. Second chapter defines the process of marketing planning and the third chapter offers basic theoretical approaches for the formulation of product, pricing, promotional and distribution strategy, which are...

Melatonin as a modulator of the gastrointestinal function of the rat

Czech Academy of Sciences Publication Activity Database

Mrnka, Libor; Soták, Matúš; Pácha, Jiří

2005-01-01

Roč. 61, č. 4 (2005), s. 602-602 ISSN 1138-7548. [European Intestinal Transport Group Meeting /20./. 24.09.2005-27.09.2005, Oléron] R&D Projects: GA ČR(CZ) GP305/03/D140 Keywords : melatonin * gastrointestinal tract * short-circuit current * melatonin receptor Subject RIV: ED - Physiology

The anti-hepatocellular carcinoma cell activity by a novel mTOR kinase inhibitor CZ415

International Nuclear Information System (INIS)

Zhang, Wei; Chen, Bingyu; Zhang, Yu; Li, Kaiqiang; Hao, Ke; Jiang, Luxi; Wang, Ying; Mou, Xiaozhou; Xu, Xiaodong; Wang, Zhen

2017-01-01

Dysregulation of mammalian target of rapamycin (mTOR) in hepatocellular carcinoma (HCC) represents a valuable treatment target. Recent studies have developed a highly-selective and potent mTOR kinase inhibitor, CZ415. Here, we showed that nM concentrations of CZ415 efficiently inhibited survival and induced apoptosis in HCC cell lines (HepG2 and Huh-7) and primary-cultured human HCC cells. Meanwhile, CZ415 inhibited proliferation of HCC cells, more potently than mTORC1 inhibitors (rapamycin and RAD001). CZ415 was yet non-cytotoxic to the L02 human hepatocytes. Mechanistic studies showed that CZ415 disrupted assembly of mTOR complex 1 (mTORC1) and mTORC2 in HepG2 cells. Meanwhile, activation of mTORC1 (p-S6K1) and mTORC2 (p-AKT, Ser-473) was almost blocked by CZ415. In vivo studies revealed that oral administration of CZ415 significantly suppressed HepG2 xenograft tumor growth in severe combined immuno-deficient (SCID) mice. Activation of mTORC1/2 was also largely inhibited in CZ415-treated HepG2 tumor tissue. Together, these results show that CZ415 blocks mTORC1/2 activation and efficiently inhibits HCC cell growth in vitro and in vivo. - Highlights: • CZ415 is anti-survival and pro-apoptotic to hepatocellular carcinoma (HCC) cells. • CZ415 inhibits HCC cell proliferation, more efficiently than mTORC1 inhibitors. • CZ415 blocks assembly and activation of both mTORC1 and mTORC2 in HCC cells. • CZ415 oral administration inhibits HepG2 tumor growth in SCID mice. • mTORC1/2 activation in HepG2 tumor is inhibited with CZ415 administration.

Keskkonnakaitsjad pakuvad välja abinõud GMO saaste vältimiseks / Ain Lember

Index Scriptorium Estoniae

Lember, Ain

2006-01-01

Eesti Keskkonnaühenduste Koda esitas ettepanekud tagamaks geneetiliselt muundatud põllumajanduskultuuride kasvatamise selliselt, et need ei tekitaks kahju tava- ja mahepõllumeestele. Vt. samas: Põllumajandusminister Ester Tuiksoo kommentaar EKO ettepanekutele. Vt. samas: Eesti ilmselt toetab geenmuundatud taimede lubamist Euroopa Liitu ja lühiinterv. põllumajandusministeeriumi taimetervise osakonna juhataja Tambet Sovaga. Vt. ka lk. 5: Nastja Pertsjonok. GMO keskkonnakaitsja pilgu läbi

Process research on non-CZ silicon material

Science.gov (United States)

1982-01-01

High risk, high payoff research areas associated with he process for producing photovoltaic modules using non-CZ sheet material are investigated. All investigations are being performed using dendritic web silicon, but all processes are directly applicable to other ribbon forms of sheet material. The technical feasibility of forming front and back junctions in non-CZ silicon using liquid dopant techniques was determined. Numerous commercially available liquid phosphorus and boron dopant solutions are investigated. Temperature-time profiles to achieve N(+) and P(+) sheet resistivities of 60 + or - 10 and 40 + or - s10 ohms per square centimeter respectively are established. A study of the optimal method of liquid dopant application is performed. The technical feasibility of forming a liquid applied diffusion mask to replace the more costly chemical vapor deposited SiO2 diffusion mask was also determined.

Characteristics and applications of ion streams produced by long-pulse lasers

Czech Academy of Sciences Publication Activity Database

Rohlena, Karel; Láska, Leoš; Jungwirth, Karel; Krása, Josef; Krouský, Eduard; Mašek, Karel; Pfeifer, Miroslav; Ullschmied, Jiří; Badziak, J.; Parys, P.; Wolowski, J.; Gammino, S.; Torrisi, L.; Boody, F. P.

2005-01-01

Roč. 47, - (2005), B503-B512 ISSN 0741-3335. [EPS Plasma Physics Conference /32nd./. Tarragona, 27.06.2005-01.07.2005] R&D Projects: GA ČR(CZ) GA202/05/2475 Institutional research plan: CEZ:AV0Z10100523 Keywords : laser plasma * ion sources * implantation Subject RIV: BH - Optics, Masers, Lasers Impact factor: 2.902, year: 2005

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific Opinion on Dietary Reference Values for phosphorus

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies derived Dietary Reference Values (DRVs) for phosphorus. The Panel considered data from balance studies, losses of phosphorus from the body and intestinal absorption for possible use in a fact......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies derived Dietary Reference Values (DRVs) for phosphorus. The Panel considered data from balance studies, losses of phosphorus from the body and intestinal absorption for possible use...... countries, which are considerably higher than the values calculated. The AI is 160 mg/day for infants (7–11 months) and between 250 and 640 mg/day for children. For adults, the AI is 550 mg/day. Taking into consideration adaptive changes in phosphorus metabolism that occur during pregnancy and lactation...

Flow and Dispersion in a Simplified Street Canyon - Wind Tunnel Study

Czech Academy of Sciences Publication Activity Database

Bezpalcová, Klára

2005-01-01

Roč. 56, č. 1 (2005), s. 2-27 ISSN 1026-2172 R&D Projects: GA ČR(CZ) GA205/04/0311; GA MŠk(CZ) OC 723.002 Institutional research plan: CEZ:AV0Z20760514 Keywords : atmospheric boundary layer * air pollution * physical modelling Subject RIV: DG - Athmosphere Sciences, Meteorology

GMO Technology. Venezuelans' Consumers Perceptions: Situation in Caracas

Directory of Open Access Journals (Sweden)

David Antonio Pereira de Abreu

2006-12-01

Full Text Available The main benefits generated by the genetically modified organisms (GMO`s include greater yields harvested, foods with greater content of nutrients, vaccines, resistant plants to virus or plagues and resistant plant to high levels of salts in ground. However, there is a controversial discussion regarding its acceptation and welcoming and is missing information in regard the topic especially in developing countries. The purpose of this study is to measure the level of knowledge or acceptance towards GMO’s and biotechnology by consumer from Caracas, Venezuela. The researchers will use a survey that was previously applied to consumers from USA, Japan and some other South American countries. The results of the study demonstrated that Venezuelan’ consumers are misinformed about this topic; however, they are more informed about microbial contamination and pesticides than other topics.

A specific endogenous reference for genetically modified common bean (Phaseolus vulgaris L.) DNA quantification by real-time PCR targeting lectin gene.

Science.gov (United States)

Venturelli, Gustavo L; Brod, Fábio C A; Rossi, Gabriela B; Zimmermann, Naíra F; Oliveira, Jaison P; Faria, Josias C; Arisi, Ana C M

2014-11-01

The Embrapa 5.1 genetically modified (GM) common bean was approved for commercialization in Brazil. Methods for the quantification of this new genetically modified organism (GMO) are necessary. The development of a suitable endogenous reference is essential for GMO quantification by real-time PCR. Based on this, a new taxon-specific endogenous reference quantification assay was developed for Phaseolus vulgaris L. Three genes encoding common bean proteins (phaseolin, arcelin, and lectin) were selected as candidates for endogenous reference. Primers targeting these candidate genes were designed and the detection was evaluated using the SYBR Green chemistry. The assay targeting lectin gene showed higher specificity than the remaining assays, and a hydrolysis probe was then designed. This assay showed high specificity for 50 common bean samples from two gene pools, Andean and Mesoamerican. For GM common bean varieties, the results were similar to those obtained for non-GM isogenic varieties with PCR efficiency values ranging from 92 to 101 %. Moreover, this assay presented a limit of detection of ten haploid genome copies. The primers and probe developed in this work are suitable to detect and quantify either GM or non-GM common bean.

Phase locking of a 2.7 THz quantum cascade laser to a microwave reference

NARCIS (Netherlands)

Khosropanah, P.; Baryshev, A.; Zhang, W.; Jellema, W.; Hovenier, J.N.; Gao, J.R.; Klapwijk, T.M.; Paveliev, D.G.; Williams, B.S.; Kumar, S.; Hu, Q.; Reno, J.L.; Klein, B.; Hesler, J.L.

2009-01-01

We demonstrate the phase locking of a 2.7 THz metal–metal waveguide quantum cascade laser (QCL) to an external microwave signal. The reference is the 15th harmonic, generated by a semiconductor superlattice nonlinear device, of a signal at 182 GHz, which itself is generated by a multiplier chain

Phase locking of a 2.7 THz quantum cascade laser to a microwave reference

NARCIS (Netherlands)

Khosropanah, P.; Baryshev, A.; Zhang, W.; Jellema, W.; Hovenier, J. N.; Gao, J. R.; Klapwijk, T. M.; Paveliev, D. G.; Williams, B. S.; Kumar, S.; Hu, Q.; Reno, J. L.; Klein, B.; Hesler, J. L.

2009-01-01

We demonstrate the phase locking of a 2.7 THz metal-metal waveguide quantum cascade laser (QCL) to an external microwave signal. The reference is the 15th harmonic, generated by a semiconductor superlattice nonlinear device, of a signal at 182 GHz, which itself is generated by a multiplier chain

Reference values for 27 clinical chemistry tests in 70-year-old males and females.

Science.gov (United States)

Carlsson, Lena; Lind, Lars; Larsson, Anders

2010-01-01

Reference values are usually defined based on blood samples from healthy men or nonpregnant women in the age range of 20-50 years. These values are not optimal for elderly patients, as many biological markers change over time and adequate reference values are important for correct clinical decisions. To validate NORIP (Nordic Reference Interval Project) reference values in a 70-year-old population. We studied 27 frequently used laboratory tests. The 2.5th and 97.5th percentiles for these markers were calculated according to the recommendations of the International Federation of Clinical Chemistry on the statistical treatment of reference values. Reference values are reported for plasma alanine aminotransferase, albumin, alkaline phosphatase, pancreas amylase, apolipoprotein A1, apolipoprotein B, aspartate aminotransferase, bilirubin, calcium, chloride, cholesterol, creatinine, creatine kinase, C-reactive protein, glucose, gamma-glutamyltransferase, HDL-cholesterol, iron, lactate dehydrogenase, LDL-cholesterol, magnesium, phosphate, potassium, sodium, transferrin, triglycerides, urate and urea. Reference values calculated from the whole population and a subpopulation without cardiovascular disease showed strong concordance. Several of the reference interval limits were outside the 90% CI of a Scandinavian population (NORIP). 2009 S. Karger AG, Basel.

Event-specific qualitative and quantitative PCR detection of the GMO carnation (Dianthus caryophyllus) variety Moonlite based upon the 5'-transgene integration sequence.

Science.gov (United States)

Li, P; Jia, J W; Jiang, L X; Zhu, H; Bai, L; Wang, J B; Tang, X M; Pan, A H

2012-04-27

To ensure the implementation of genetically modified organism (GMO)-labeling regulations, an event-specific detection method was developed based on the junction sequence of an exogenous integrant in the transgenic carnation variety Moonlite. The 5'-transgene integration sequence was isolated by thermal asymmetric interlaced PCR. Based upon the 5'-transgene integration sequence, the event-specific primers and TaqMan probe were designed to amplify the fragments, which spanned the exogenous DNA and carnation genomic DNA. Qualitative and quantitative PCR assays were developed employing the designed primers and probe. The detection limit of the qualitative PCR assay was 0.05% for Moonlite in 100 ng total carnation genomic DNA, corresponding to about 79 copies of the carnation haploid genome; the limit of detection and quantification of the quantitative PCR assay were estimated to be 38 and 190 copies of haploid carnation genomic DNA, respectively. Carnation samples with different contents of genetically modified components were quantified and the bias between the observed and true values of three samples were lower than the acceptance criterion (GMO detection method. These results indicated that these event-specific methods would be useful for the identification and quantification of the GMO carnation Moonlite.

Prevalence of hypersensitivity to dental local anesthetic drugs in patients referred to Tehran Allergy clinic (2005-2007

Directory of Open Access Journals (Sweden)

Akhlaghi F.

2009-03-01

Full Text Available "nBackground and Aim: According to controversies in the prevalence of hypersensitivity to dental local anesthetic drugs and patients who claim hypersensitivity to these drugs, the aim of this study was to determine the prevalence of hypersensitivity to dental amide local anesthetic drugs in patients referred to Tehran Allergy Clinic in 2005-2007. "nMaterials and Methods: In this Study (Review of existing data, records of 130 patients who were referred to "Tehran allergy Clinic" (2005-2007 were studied. "nResults: The average age of patients was 29.5±18.8 years. 34% of cases showed positive skin reactions to at least one of the tested Lidocain concentrations and 10% of cases showed positive skin reactions to at least one of the tested Prilocain concentrations. There was a statistically significant difference in hypersensitivity to Lidocain 0.01 and 0.001 (p=0.017 and also between Lidocain 0.001 and 0.0001 (p<0.01. There was no statistically significant difference between other tested drug concentrations (p>0.05. "nConclusion: Many patients with history of hypersensitivity, show positive reaction to local dental anesthetic drugs. Prilocain hypersensitivity reactions are less than Lidocain. So application of Prilocain accompanies with less risk but its application should not be considered completely safe.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for biotin

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for biotin. Biotin is a water-soluble vitamin which serves as a co-factor for several carboxylases that play critical roles in the synthesis...

Behavioral deficits in adult rats treated neonatally with glutamate

Czech Academy of Sciences Publication Activity Database

Hliňák, Zdeněk; Gandalovičová, D.; Krejčí, I.

2005-01-01

Roč. 27, č. 3 (2005), s. 465-473 ISSN 0892-0362 R&D Projects: GA MZd(CZ) NF6474 Institutional research plan: CEZ:AV0Z5011922 Keywords : neonatal treatment * monosodium glutamate * long-term effect Subject RIV: ED - Physiology Impact factor: 1.940, year: 2005

The current state of GMO governance: are we ready for GM animals?

Science.gov (United States)

Vàzquez-Salat, Núria; Salter, Brian; Smets, Greet; Houdebine, Louis-Marie

2012-01-01

Given the history of GMO conflict and debate, the GM animal future is dependent on the response of the regulatory landscape and its associated range of interest groups at national, regional and international levels. Focusing on the EU and the USA, this article examines the likely form of that multi-level response, the increased role of cultural values, the contribution of new and existing interest groups and the consequent implications for the commercialization of both green and red GM animal biotechnology. Copyright © 2012. Published by Elsevier Inc.

Putting problem formulation at the forefront of GMO risk analysis.

Science.gov (United States)

Tepfer, Mark; Racovita, Monica; Craig, Wendy

2013-01-01

When applying risk assessment and the broader process of risk analysis to decisions regarding the dissemination of genetically modified organisms (GMOs), the process has a tendency to become remarkably complex. Further, as greater numbers of countries consider authorising the large-scale dissemination of GMOs, and as GMOs with more complex traits reach late stages of development, there has been increasing concern about the burden posed by the complexity of risk analysis. We present here an improved approach for GMO risk analysis that gives a central role to problem formulation. Further, the risk analysis strategy has been clarified and simplified in order to make rigorously scientific risk assessment and risk analysis more broadly accessible to diverse stakeholder groups.

Genetic algorithms for thyroid gland ultrasound image feature reduction

Czech Academy of Sciences Publication Activity Database

Tesař, Ludvík; Smutek, D.; Jiskra, J.

2005-01-01

Roč. 3612, č. - (2005), s. 841-844 ISSN 0302-9743. [International Conference ICNC 2005 /1./. Changsha, 27.08.2005-29.08.2005] R&D Projects: GA AV ČR 1ET101050403 Institutional research plan: CEZ:AV0Z10750506 Keywords : medical imaging * classification * Bayes classifier * Huzzolini feature * pattern recognition Subject RIV: BB - Applied Statistics, Operational Research http://library.utia.cas.cz/prace/20050229.pdf

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific Opinion on the risks to animal and public health and the environment related to the presence of nickel in feed

OpenAIRE

Petersen, Annette

2015-01-01

Following a request from the European Commission, the risks to animal and human health and the environment related to the presence of nickel (Ni) in feed were assessed by the EFSA Panel on Contaminants in the Food Chain (CONTAM Panel). The presence of Ni in feed can arise from both natural and anthropogenic sources. Additionally, certain feed materials contain metallic Ni, since it is used as a catalyst in their production. Based on the differences observed between the Ni exposure levels esti...

Involvement of AMP - activated protein kinase in fat depot-specific metabolic changes during starvation

Czech Academy of Sciences Publication Activity Database

Šponarová, Jana; Mustard, K. J.; Horáková, Olga; Flachs, Pavel; Rossmeisl, Martin; Brauner, Petr; Bardová, Kristina; Thomason-Hughes, M.; Braunerová, Radka; Janovská, Petra; Hardie, D. G.; Kopecký, Jan

2005-01-01

Roč. 579, č. 27 (2005), s. 6105-6110 ISSN 0014-5793 R&D Projects: GA ČR(CZ) GA303/05/2580; GA AV ČR(CZ) KJB5011303 Grant - others:Wellcome Trust(GB) 02760; European Commission(XE) LSHM-CT-2004-005272; Diabetes UK(GB) Project Grant; Biotechnology and Biological Sciences Research Council(GB) Research Studentship; GA-(GB) Novo-Nordisk Institutional research plan: CEZ:AV0Z50110509 Keywords : lipid metabolism * AMPK * starvation Subject RIV: ED - Physiology Impact factor: 3.415, year: 2005

CzEngVallex: a Bilingual Czech-English Valency Lexicon

Directory of Open Access Journals (Sweden)

Urešová Zdeňka

2016-04-01

Full Text Available This paper introduces a new bilingual Czech-English verbal valency lexicon (called CzEng-Vallex representing a relatively large empirical database. It includes 20,835 aligned valency frame pairs (i.e., verb senses which are translations of each other and their aligned arguments. This new lexicon uses data from the Prague Czech-English Dependency Treebank and also takes advantage of the existing valency lexicons for both languages: the PDT-Vallex for Czech and the EngVallex for English. The CzEngVallex is available for browsing as well as for download in the LINDAT/CLARIN repository.

Strategy of development and online marketing for website www.skutecnydarek.cz

OpenAIRE

Fodor, Aleš

2011-01-01

The goal of this bachelor's thesis is to suggest particular online marketing strategy for People in need's website - www.skutecnydarek.cz. Firstly the thesis is going to aim at analysing the present condition of marketing activities and the quality of the web site for www.skutecnydarek.cz. Then the online marketing strategy is going be developed.

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific Opinion on nitrofurans and their metabolites in food

DEFF Research Database (Denmark)

Petersen, Annette

and carcinogenic and, also, have non-neoplastic effects in animals. Margins of exposure (MOEs) were calculated at 2.0 × 105 or greater for carcinogenicity and at 2.5 × 103 or greater for non-neoplastic effects. The CONTAM Panel concluded that it is unlikely that exposure to food contaminated with nitrofuran marker...... metabolites at or below 1.0 µg/kg is a health concern. A scenario in which foods are considered to be contaminated with semicarbazide, from use of carrageenan as a food additive, at 1 µg/kg was used to assess whether it is appropriate to apply the RPA to foods of non-animal origin; MOEs of greater than 104......Nitrofurans are antimicrobial agents not authorised for use in food-producing animals in the European Union. Nitrofurans are rapidly metabolised, occurring in animal tissues as protein-bound metabolites. The European Commission requested EFSA to provide a scientific opinion on the risks to human...

Modulation of murine cellular immune response and cytokine production by salivary gland lysate of three sand fly species

Czech Academy of Sciences Publication Activity Database

Rohoušová, Iva; Volf, P.; Lipoldová, Marie

2005-01-01

Roč. 12, č. 27 (2005), s. 469-473 ISSN 0141-9838 R&D Projects: GA ČR(CZ) GA310/03/1381 Institutional research plan: CEZ:AV0Z5052915 Keywords : cytokine production * Lutzomyia * Phlebotomus Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.445, year: 2005

EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2016 Scientific Opinion on Flavouring Group Evaluation 75, Revision 1 (FGE.75Rev1): Consideration of tetrahydrofuran derivatives evaluated by JECFA (63rd meeting) structurally related to tetrahydrofuran

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Nørby, Karin Kristiane

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF) of the EFSA was requested to consider evaluations of flavouring substances assessed since 2000 by the Joint FAO/WHO Expert Committee on Food Additives (the JECFA), and to decide whether further evaluation is neces......The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF) of the EFSA was requested to consider evaluations of flavouring substances assessed since 2000 by the Joint FAO/WHO Expert Committee on Food Additives (the JECFA), and to decide whether further evaluation...... for anhydrolinalool oxide (5) [FL-no: 13.097]. The substances were evaluated through a stepwise approach that integrates information on structure-activity relationships, intake from current uses, toxicological threshold of concern, and available data on metabolism and toxicity. The JECFA concluded all the 11...... with the JECFA conclusion ‘No safety concern at estimated level of intake as flavouring substances’ based on the maximised survey-derived daily intake (MSDI) approach. The specifications for the materials of commerce have also been considered and for all 11 substances, the information is adequate....

Hybrid regimes of knowledge? Challenges for constructing scientific evidence in the context of the GMO-debate.

Science.gov (United States)

Böschen, Stefan

2009-07-01

Over the last two decades, there has been a remarkable shift of attention to the scientific and political fundamentals of the precautionary principle. The application of this principle has become a main strategy of coping with the different forms and problems related to non-knowledge. Thus, societies are increasingly confronted with the challenging and hitherto unresolved problem of political and technological decision-making under conditions of diverging framings of non-knowledge. At present, there seems to be no generally accepted scientific or institutional approach. This is why the fundamental question of how different scientific actors define and construct evidence is not answered yet. Hence, this paper is based on the consideration that the conflicts in risk policy concerning genetically modified organisms (GMO) depend on the unresolved conflicts about the diverging scientific strategies and structures of evidence-making between the epistemic cultures involved. Thus, this study investigates two questions: (1) do the epistemic strategies of evidence-making differ systematically with the scientific actors involved in the GMO-debate? (2) What consequences emerge considering institutionalized procedures of decision-making? This article is based on a secondary analysis of findings and perspectives reported in the literature and on the methods of qualitative social empirical research, i.e., interviews with experts. A total number of 34 interviews were conducted to explore the different strategies of handling non-knowledge and constructing evidence. Actors from science, administration, business and NGOs were interviewed. In this way, typical epistemic cultures can be described. An epistemic culture is the constellation of methodological strategies, theoretical assumptions and practical-experimental settings which define in every speciality the ways how we know what we know. There are two main results. Firstly, it was worked out that the epistemic cultures involved

Morphometry and growth of three .i.Synechococcus./i.-like picoplanktic cyanobacteria at different culture conditions

Czech Academy of Sciences Publication Activity Database

Jezberová, Jitka; Komárková, J.

2007-01-01

Roč. 578, č. 1 (2007), s. 17-27 ISSN 0018-8158. [Workshop of the International Association of Phytoplankton Taxonomy and Ecology (IAP) /14./. Sapanca, 01.09.2005-11.09.2005] R&D Projects: GA AV ČR(CZ) IBS6017004; GA ČR(CZ) GA206/05/0007 Grant - others:EU(XE) EVK2-1999-00213 Institutional research plan: CEZ:AV0Z60170517 Keywords : cell elongation * crossed gradients * cyanobacteria * Cyanobium * involution cells * light * morphometry * nutrients * Synechococcus * temperature Subject RIV: EE - Microbiology, Virology Impact factor: 1.201, year: 2007

Study of Capillary Condensation of Butane in Vycor Membrane

Czech Academy of Sciences Publication Activity Database

Uchytil, Petr; Petričkovič, Roman; Seidel-Morgenstern, A.

2005-01-01

Roč. 264, 1-2 (2005), s. 27-36 ISSN 0376-7388 R&D Projects: GA AV ČR(CZ) IAA4072402 Institutional research plan: CEZ:AV0Z40720504 Keywords : capillary condensation * mass transport * porous membrane Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 2.654, year: 2005

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to Lactobacillus rhamnosus ATCC 53103 (LGG) and “gastro-intestinal health” (ID 906) and maintenance of tooth mineralisation (ID 3018) pursuant to Article 13

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to Lactobacillus rhamnosus ATCC 53103 (LGG) and “gastrointestinal health” and maintenance of tooth mineralisation. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has...... received from Member States or directly from stakeholders. The food constituent that is the subject of the health claims is L. rhamnosus ATCC 53103 (LGG). The Panel considers that L. rhamnosus ATCC 53103 (LGG) is sufficiently characterised....

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to chitosan and reduction in body weight (ID 679, 1499), maintenance of normal blood LDL-cholesterol concentrations (ID 4663), reduction of intestinal transit time (ID

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to chitosan and reduction in body weight, maintenance of normal blood LDL-cholesterol concentrations, reduction of intestinal transit time and reduction of inflammation. The scientific substantiation is based on the information provided by the Member States in the consolidated list...... of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claim is chitosan. The Panel considers that chitosan is sufficiently characterised....

Acute tier-1 and tier-2 effect assessment approaches in the EFSA Aquatic Guidance Diocument: are they sufficiently protective for insecticides?

NARCIS (Netherlands)

Wijngaarden, van R.P.A.; Maltby, L.; Brock, T.C.M.

2015-01-01

BACKGROUND The objective of this paper is to evaluate whether the acute tier-1 and tier-2 methods as proposed by the Aquatic Guidance Document recently published by the European Food Safety Authority (EFSA) are appropriate for deriving regulatory acceptable concentrations (RACs) for insecticides.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for pantothenic acid

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for pantothenic acid. Pantothenic acid is a water-soluble vitamin, which is a component of coenzyme A (CoA) and acyl-carrier proteins. Pantothenic...

Analytical validation of a reference laboratory ELISA for the detection of feline leukemia virus p27 antigen.

Science.gov (United States)

Buch, Jesse S; Clark, Genevieve H; Cahill, Roberta; Thatcher, Brendon; Smith, Peter; Chandrashekar, Ramaswamy; Leutenegger, Christian M; O'Connor, Thomas P; Beall, Melissa J

2017-09-01

Feline leukemia virus (FeLV) is an oncogenic retrovirus of cats. Immunoassays for the p27 core protein of FeLV aid in the detection of FeLV infections. Commercial microtiter-plate ELISAs have rapid protocols and visual result interpretation, limiting their usefulness in high-throughput situations. The purpose of our study was to validate the PetChek FeLV 15 ELISA, which is designed for the reference laboratory, and incorporates sequential, orthogonal screening and confirmatory protocols. A cutoff for the screening assay was established with 100% accuracy using 309 feline samples (244 negative, 65 positive) defined by the combined results of FeLV PCR and an independent reference p27 antigen ELISA. Precision of the screening assay was measured using a panel of 3 samples (negative, low-positive, and high-positive). The intra-assay coefficient of variation (CV) was 3.9-7.9%; the inter-assay CV was 6.0-8.6%. For the confirmatory assay, the intra-assay CV was 3.0-4.7%, and the inter-assay CV was 7.4-9.7%. The analytical sensitivity for p27 antigen was 3.7 ng/mL for inactivated whole FeLV and 1.2 ng/mL for purified recombinant FeLV p27. Analytical specificity was demonstrated based on the absence of cross-reactivity to related retroviruses. No interference was observed for samples containing added bilirubin, hemoglobin, or lipids. Based on these results, the new high-throughput design of the PetChek FeLV 15 ELISA makes it suitable for use in reference laboratory settings and maintains overall analytical performance.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on Dietary Reference Values for vitamin C

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for vitamin C. The Panel concludes that an Average Requirement (AR) can be derived from indicators of vitamin C status, as well as a Population...... Reference Intake (PRI) assuming a coefficient of variation (CV) of 10 %. Several health outcomes possibly associated with vitamin C intake were also considered but data were found to be insufficient to establish DRVs. For healthy adults, the AR is determined from the quantity of vitamin C that balances...... metabolic vitamin C losses and allows the maintenance of an adequate body pool characterised by fasting plasma ascorbate concentrations at around 50 µmol/L. In men, an AR of 90 mg/day of vitamin C and a PRI of 110 mg/day are proposed. As no value for metabolic losses is available in women, the AR for women...

Agrobacterium rhizogenes-Mediated Transformation – a Non-GMO Platform For Developing Compact Ornamentals

DEFF Research Database (Denmark)

Lütken, Henrik Vlk; Hegelund, Josefine Nymark; Lauridsen, Uffe Bjerre

of these compounds are potentially harmful to both the environment and human health. A new non-GMO molecular breeding strategy, as opposed to both the application of chemical growth retardants and conventional molecular breeding is Agrobacterium rhizogenes-mediated transformation. In this method, the soil borne...... for transformations, plants produced via this approach are not considered as GMOs in the European Union and Japan. We have developed an optimised Agrobacterium rhizogenes-mediated transformation platform useful for a wide range of ornamentals. Kalanchoë was the starting point and the effect of the rol-genes has now...

Guidance on a harmonised framework for pest risk assessment and the identification and evaluation of pest risk management options by EFSA

DEFF Research Database (Denmark)

Baker, R.; Candresse, T.; Dormannsné Simon, E.

2010-01-01

The Scientific Panel on Plant Health was requested by EFSA to develop a guidance document on a harmonised framework for risk assessment of organisms harmful to plants and plant products and the identification and evaluation of risk management options. The document provides guiding principles on a...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to folate and maintenance of normal blood pressure (ID 176) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to folate and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to xanthan gum and changes in bowel function (ID 837) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to xanthan gum and changes in bowel function. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to spermidine and contribution to normal hair growth (ID 1705) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to spermidine and contribution to normal hair growth. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders...

Legal and Illegal Colours

DEFF Research Database (Denmark)

Larsen, John Christian

2008-01-01

opinions on food additives, including colours, and on the bioavailability and safety of nutrient sources. The WG ADD consists of several members from the AFC Panel together with selected external experts. The draft opinions go forward to the AFC Panel for discussion and final adoption. The adopted opinions......://www.efsa.europa.eu/EFSA/efsa_locale-1178620753812_1178620761956.htm. Accessed 12.05.08.] this paper only deals with some of the major issues that the Panel has faced in relation to the use of food colours. The three topics to be dealt with are (1) evaluation of illegal colours in food in the EU (EFSA, 2005), (2) re-evaluation of the authorised...... food colours in the EU (ongoing, but one opinion on Red 2G has been published; EFSA, 2007), and (3) evaluation of 'the Southampton study' on hyperactivity in children after intake of food colours (and sodium benzoate) (ongoing at the time of this presentation, but an opinion has now been published...

The influence of feeding GMO-peas on growth of animal models

Directory of Open Access Journals (Sweden)

Petr Mares

2014-02-01

Full Text Available Introduction of genetically modified (GM food or feed into the commercial sale represents a very complicated process. One of the most important steps in approval process is the evaluation of all risks on the health status of people and animal models. Within our project the genetically modified peas was breeded that showed significant resistance against Pea seed-borne mosaic virus and Pea enation mosaic virus. Preclinical studies have been conducted to found out the effect of GMO peas on animals - rats of outbreeding line Wistar. In a total, 24 male, specific pathogen free Wistar rats were used in the experiment. At the beginning of the experiment, the animals were 28 days old. The three experimental groups with 8 individuals were created. The first group of rats was fed with GMO peas, the second group of rats consumed mix of pea cultivar Raman and the third group was control without pea addition (wheat and soya were used instead of pea. In the present study we focused our attention on health, growth and utility features of rats fed with GM pea. All characteristic were observed during the experiment lasting 35 days. Consumed feed was weighted daily and the weight of the animals was measured every seven days. The average values were compared within the groups. The aim of the experiment was to verify if resistant lines of pea influence the weight growth of animal models. The results of our experiment showed that even a high concentration (30% of GM pea did not influence growth rate of rats to compare with both rats fed with pea of Raman cultivar and control group. We did not observe any health problems of animal models during the experiment.

Stimulus reference frame and neural coding precision

Czech Academy of Sciences Publication Activity Database

Košťál, Lubomír

2016-01-01

Roč. 71, Apr 2016 (2016), s. 22-27 ISSN 0022-2496 R&D Projects: GA ČR(CZ) GA15-08066S Institutional support: RVO:67985823 Keywords : Fisher information * coding accuracy * measurement scale * Jeffreys prior Subject RIV: FH - Neurology Impact factor: 1.377, year: 2016

Ontologies and Information Systems: A Literature Survey

Science.gov (United States)

2011-06-01

Falcon-AO (LMO + GMO ) [146], and RiMOM [317]. Meta-matching systems include APFEL [76] and eTuner [286]. There also exist frameworks that provide a set...Jian, N., Qu, Y. and Wang, Q. 2005. GMO : A graph matching for ontologies. In Proceedings of the K-CAPWorkshop on Integrating Ontologies, Banff

Eating nanomaterials: cruelty-free and safe? the EFSA guidance on risk assessment of nanomaterials in food and feed.

Science.gov (United States)

Sauer, Ursula G

2011-12-01

Nanomaterials are increasingly being added to food handling and packaging materials, or directly, to human food and animal feed. To ensure the safety of such engineered nanomaterials (ENMs), in May 2011, the European Food Safety Authority (EFSA) published a guidance document on Risk assessment of the application of nanoscience and nanotechnologies in the food and feed chain. It states that risk assessment should be performed by following a step-wise procedure. Whenever human or animal exposure to nanomaterials is expected, the general hazard characterisation scheme requests information from in vitro genotoxicity, toxicokinetic and repeated dose 90-day oral toxicity studies in rodents. Numerous prevailing uncertainties with regard to nanomaterial characterisation and their hazard and risk assessment are addressed in the guidance document. This article discusses the impact of these knowledge gaps on meeting the goal of ensuring human safety. The EFSA's guidance on the risk assessment of ENMs in food and animal feed is taken as an example for discussion, from the point of view of animal welfare, on what level of uncertainty should be considered acceptable for human safety assessment of products with non-medical applications, and whether animal testing should be considered ethically acceptable for such products.

Phase Locking of a 2.7 THz Quantum Cascade Laser to a Microwave Reference

Science.gov (United States)

Khosropanah, P.; Baryshev, A.; Zhang, W.; Jellema, W.; Hovenier, J. N.; Gao, J. R.; Klapwijk, T. M.; Paveliev, D. G.; Williams, B. S.; Hu, Q.;

Applicability of Three Alternative Instruments for Food Authenticity Analysis: GMO Identification

Science.gov (United States)

Burrell, A.; Foy, C.; Burns, M.

2011-01-01

Ensuring foods are correctly labelled for ingredients derived from genetically modified organisms (GMOs) is an issue facing manufacturers, retailers, and enforcement agencies. DNA approaches for the determination of food authenticitys often use the polymerase chain reaction (PCR), and PCR products can be detected using capillary or gel electrophoresis. This study examines the fitness for purpose of the application of three laboratory electrophoresis instruments (Agilent Bioanalyzer 2100, Lab901 TapeStation, and Shimadzu MCE-202 MultiNA) for the detection of GMOs using PCR based on a previously validated protocol. Whilst minor differences in the performance characteristics of bias and precision were observed, all three instruments demonstrated their applicability in using this protocol for screening of GMO ingredients. PMID:21527985

Chinese modify CZ-2/3 rocket boosters, focus on commercial launch market

Science.gov (United States)

Covault, C.

1985-07-01

A program underway in the People's Republic of China to modify the Titan-class CZ-2/3 satellite-launch and ICBM boosters is described on the basis of a recent visit to the manufacturing plant in Shanghai. The present two-stage CZ-2 and three-stage CZ-3 can place 5000 lbs in LEO or 3080 lbs in GEO, respectively, and are produced on a custom basis with a delivery time of about 2 yrs. Modifications introduced include 4 x 6-ft fins and a pogo-suppression system for the four-engine first stage and a steel support band for the combustion chamber of the 80-ton-thrust second-stage main engine.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to citrulline-malate and faster recovery from muscle fatigue after exercise pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Biocodex, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scienti......Following an application from Biocodex, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...... on the scientific substantiation of a health claim related to citrulline-malate and faster recovery from muscle fatigue after exercise. The Panel considers that citrulline-malate is sufficiently characterised. The claimed effect proposed by the applicant is “improved recovery from muscle fatigue”. Faster recovery...... function. The evidence provided by the applicant did not establish that a faster reduction of blood lactate concentrations through a dietary intervention leads to faster recovery from muscle fatigue by contributing to the restoration of muscle function after exercise. No conclusions could be drawn from...

Akutní infarkt myokardu u pacientů s diabetem a nediabetiků. Podle dat z registru ALERT-CZ

Czech Academy of Sciences Publication Activity Database

Hanuš, P.; Reissigová, Jindra; Grünfeldová, H.; Svobodová, I.; Janský, P.; Monhart, Z.; Widimský, P.; Vojáček, J.; Zvárová, Jana

2010-01-01

Roč. 52, č. 9 (2010), s. 578-578 ISSN 0010-8650. [Konference České společnosti pro hypertenzi /27./, Konference pracovní skupiny Preventivní kardiologie ČKS /19./, Konference pracovní skupiny Srdeční selhání ČKS /15./. 07.10.2010-09.10.2010, Mariánské Lázně] R&D Projects: GA MŠk(CZ) 1M06014 Institutional research plan: CEZ:AV0Z10300504 Keywords : akutní infarkt myokardu * diabetes mellitus * rizikové faktory aterosklerózy Subject RIV: IN - Informatics, Computer Science

Návrh a vyhodnocení online propagace projektu Qool.cz

OpenAIRE

Bujalková, Jana

2012-01-01

This diploma thesis is focused on internet and mobile advertising and its usage in promoting the Qool.cz project. The beginning of thesis is dedicated to general possibilities of using mobile advertising, possible payment methods and advertising formats. Throughout the whole thesis, there is a focus on three main parts of the mobile environment - mobile web, mobile applications and mobile advertising. Practical part of the thesis focuses on using all these methods in promoting the Qool.cz pro...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the essential composition of infant and follow-on formulae

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on the essential composition of infant and follow-on formula. This opinion reviews the opinion provided by the Scientific Committee on Food...... in 2003 on the essential requirements of infant and follow-on formulae in light of more recent evidence and by considering the Panel’s opinion of October 2013 on nutrient requirements and dietary intakes of infants and young children in the European Union. The minimum content of a nutrient in formula...... proposed in this opinion is derived from the intake levels the Panel had considered adequate for the majority of infants in the first six months of life in its previous opinion and an average amount of formula consumed during this period. From a nutritional point of view, the minimum contents of nutrients...

DPOD2005: An extension of ITRF2005 for Precise Orbit Determination

Science.gov (United States)

Willis, P.; Ries, J. C.; Zelensky, N. P.; Soudarin, L.; Fagard, H.; Pavlis, E. C.; Lemoine, F. G.

2009-09-01

For Precise Orbit Determination of altimetry missions, we have computed a data set of DORIS station coordinates defined for specific time intervals called DPOD2005. This terrestrial reference set is an extension of ITRF2005. However, it includes all new DORIS stations and is more reliable, as we disregard stations with large velocity formal errors as they could contaminate POD computations in the near future. About 1/4 of the station coordinates need to be defined as they do not appear in the original ITRF2005 realization. These results were verified with available DORIS and GPS results, as the integrity of DPOD2005 is almost as critical as its accuracy. Besides station coordinates and velocities, we also provide additional information such as periods for which DORIS data should be disregarded for specific DORIS stations, and epochs of coordinate and velocity discontinuities (related to either geophysical events, equipment problem or human intervention). The DPOD model was tested for orbit determination for TOPEX/Poseidon (T/P), Jason-1 and Jason-2. Test results show DPOD2005 offers improvement over the original ITRF2005, improvement that rapidly and significantly increases after 2005. Improvement is also significant for the early T/P cycles indicating improved station velocities in the DPOD2005 model and a more complete station set. Following 2005 the radial accuracy and centering of the ITRF2005-original orbits rapidly degrades due to station loss.

Application of Transgenic Technologies to Papaya: Developments and Biosafety Assessments in Thailand

Czech Academy of Sciences Publication Activity Database

Kertbundit, Sunee; Juříček, Miloslav

2010-01-01

Roč. 4, č. 1 (2010), s. 52-57 ISSN 1749-0413 Institutional research plan: CEZ:AV0Z50380511 Keywords : coat protein-mediated resistance * GMO * Papaya ringspot virus Subject RIV: EF - Botanics http://home.ueb.cas.cz/publikace/2010_Kertbundit_TransgenicPlantJournal_52.pdf

Sowing the seeds of skepticism: Russian state news and anti-GMO sentiment.

Science.gov (United States)

Dorius, Shawn F; Lawrence-Dill, Carolyn J

2018-03-21

Biotech news coverage in English-language Russian media fits the profile of the Russian information warfare strategy described in recent military reports. This raises the question of whether Russia views the dissemination of anti-GMO information as just one of many divisive issues it can exploit as part of its information war, or if GMOs serve more expansive disruptive purposes. Distinctive patterns in Russian news provide evidence of a coordinated information campaign that could turn public opinion against genetic engineering. The recent branding of Russian agriculture as the ecologically clean alternative to genetically engineered foods is suggestive of an economic motive behind the information campaign against western biotechnologies.

How Precisely can we Determine the $\\piNN$ Coupling Constant from the Isovector GMO Sum Rule?

CERN Document Server

Loiseau, B; Thomas, A W

1999-01-01

The isovector GMO sum rule for zero energy forward pion-nucleon scattering iscritically studied to obtain the charged pion-nucleon coupling constant usingthe precise negatively charged pion-proton and pion-deuteron scattering lengthsdeduced recently from pionic atom experiments. This direct determination leadsto a pseudoscalar charged pion-nucleon coupling constant of 14.23 +- 0.09(statistic) +- 0.17 (systematic). We obtain also accurate values for thepion-nucleon scattering lengths.

Registration document 2005

International Nuclear Information System (INIS)

2005-01-01

This reference document of Gaz de France provides information and data on the Group activities in 2005: financial informations, business, activities, equipments factories and real estate, trade, capital, organization charts, employment, contracts and research programs. (A.L.B.)

Multiple batch recharging for industrial CZ silicon growth

Science.gov (United States)

Fickett, B.; Mihalik, G.

2001-05-01

The Czochralski (CZ) crystal growth process used in the Siemens Solar Industries’ (SSI) Vancouver, WA facility was non-continuous. Each furnace run's production was limited by the size of the starting charge. Once the charge was depleted, the furnace was shut down, cooled, and set back up for the next run. A recharge system was developed which transforms standard CZ growth into a semi-continuous process. Now when the charge is depleted, the crucible can be refilled in situ as the grown ingot is being removed from the furnace. SSI has demonstrated up to 14 recharge cycles in a single run. The resulting benefits included: significant cost reduction, increased yield, increased throughput, reduced energy consumption, improved process capability, reduced material handling requirements, and reduced labor. The recharge system also enables the use of granular silicon, which requires less than 30% of the energy required when manufacturing silicon-starting materials. This significantly reduces the energy “pay-back” time associated with SSI's finished product, photovoltaic panels.

End-of-year-closure 2005/2006

CERN Multimedia

HR Department

2005-01-01

As announced in Weekly Bulletin Nº 2/2005, the Laboratory will be closed from Thursday 22 December 2005 to Wednesday 4 January 2006 inclusive. This period consists of 14 days: 4 days' official holiday, i.e. 24, 25 and 31 December 2005 and 1st January 2006; 6 days' special paid leave in accordance with Article R II 4.34 of the Staff Regulations, i.e.  26, 27, 28, 29, 30 December 2005 and 4 January 2006; 4 days, 22 and 23 December 2005 to compensate for 24 and 25 December 2005 and 2 and 3 January 2006 to compensate for 31 December 2005 and 1st January 2006 (Article R II 4.33 of the Staff Regulations); The first working day in the New Year will be Thursday, 5 January 2006. Further information is available from Department Secretariats, specifically concerning the conditions applicable to members of the personnel who are required to work during this period. Human Resources Department Tel. 73903

End-of-year-closure 2005/2006

CERN Multimedia

2005-01-01

As announced in Weekly Bulletin No. 2/2005, the Laboratory will be closed from Thursday 22 December 2005 to Wednesday 4 January 2006 inclusive. This period consists of 14 days: 4 days' official holiday, i.e. 24, 25 and 31 December 2005 and 1st January 2006; 6 days' special paid leave in accordance with Article R II 4.34 of the Staff Regulations, i.e.  26, 27, 28, 29, 30 December 2005 and 4 January 2006; 4 days, 22 and 23 December 2005 to compensate for 24 and 25 December 2005 and 2 and 3 January 2006 to compensate for 31 December 2005 and 1st January 2006 (Article R II 4.33 of the Staff Regulations); The first working day in the New Year will be Thursday, 5 January 2006. Further information is available from Department Secretariats, specifically concerning the conditions applicable to members of the personnel who are required to work during this period. Human Resources Department Tel. 73903

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013 . Scientific opinion on Dietary Reference Values for fluoride

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for fluoride, which are provided as Adequate Intake (AI) from all sources, including non-dietary sources. Fluoride is not an essential nutrient...... and adults, including pregnant and lactating women. For pregnant and lactating women, the AI is based on the body weight before pregnancy and lactation. Reliable and representative data on the total fluoride intake of the European population are not available. © European Food Safety Authority, 2013...

ELAG2005

CERN Multimedia

2005-01-01

The 29th ELAG (European Library Automation Group)Seminar took place at CERN from 1st to 4th June. Once a year the Group brings together people involved in library automation in the leading European libraries and information centres. The organization has 450 members from 27 countries all over Europe. In the last few years over 100 people have attended the seminars. This year's very successful event was devoted to Open Access, a very hot topic these days. The 120 participants were enrolled in nine different workshops, covering several topics ranging from inter-library loans to RSS, e-books and many more. The seminar was sponsored by ExLibris Ltd., the Institute of Physics, VTLS Inc., Elsevier and Ebsco. Further information can be found at http://elag2005.web.cern.ch/elag2005

EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids) , 2016. Scientific Opinion on Flavouring Group Evaluation 90, Revision 1 (FGE.90Rev1): consideration of six substances evaluated by JECFA (68th meeting) structurally related to aliphatic, alicyclic and aromatic saturated and unsaturated tertiary alcohols, aromatic tertiary alcohols and their esters evaluated by EFSA in FGE.18Rev1 and FGE.75Rev1

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Nørby, Karin Kristiane

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of EFSA was requested to consider evaluations of flavouring substances assessed since 2000 by the Joint FAO/WHO Expert Committee on Food Additives (the JECFA), and to decide whether further evaluation is necessary...... refined exposure estimation and to judge whether a re-evaluation according to the Procedure is needed....

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sugar-free chewing gum sweetened with xylitol and plaque acid neutralisation (ID 485), maintenance of tooth mineralisation (ID 486, 562, 1181), reduction of dental

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to sugar-free chewing gum sweetened with xylitol and plaque acid neutralisation, maintenance of tooth mineralisation, reduction of dental plaque, and defence against pathogens in the middle ear. The scientific substantiation is based on the information provided by the Member States...... in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food that is the subject of the health claims is sugar-free chewing gum sweetened with xylitol. The Panel considers that sugar-free chewing gum sweetened with xylitol...

Changing composition of human capital: the Czech Republic, Hungary, and Poland

Czech Academy of Sciences Publication Activity Database

Jeong, Byeongju; Kejak, Michal; Vinogradov, Viatcheslav

-, č. 248 (2005), s. 1-27 ISSN 1211-3298 Institutional research plan: CEZ:AV0Z70850503 Keywords : human capital * composition * occupation Subject RIV: AH - Economics http://www.cerge-ei.cz/pdf/wp/Wp248.pdf

Reference Electrodes Based on Solid Amalgams

Czech Academy of Sciences Publication Activity Database

Josypčuk, Bohdan; Novotný, Ladislav

2004-01-01

Roč. 16, č. 3 (2004), s. 238-241 ISSN 1040-0397 Grant - others:GIT(AR) 101/02/U111/CZ Institutional research plan: CEZ:AV0Z4040901 Keywords : solid amalgam * reference electrode * voltammetry Subject RIV: CG - Electrochemistry Impact factor: 2.038, year: 2004

Recommendations from a meeting on health implications of genetically modified organism (GMO).

Science.gov (United States)

Amofah, George

2014-06-01

The Ghana Public Health Association organized a scientific seminar to examine the introduction of genetically modified organisms into public use and the health consequences. The seminar was driven by current public debate on the subject. The seminar identified some of the advantages of GMOs and also the health concerns. It is clear that there is the need to enhance local capacity to research the introduction and use of GMOs; to put in place appropriate regulatory mechanisms including particularly the labeling of GMO products and post-marketing surveillance for possible negative health consequences in the long term. Furthermore the appropriate state agency should put in place advocacy strategies to keep the public informed about GMOs.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the modification of the authorisation of a health claim related to cocoa flavanols and maintenance of normal endotheliumdependent vasodilation pursuant to Article 13(5) of Regulation (EC) No 1924

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following an application from Barry Callebaut Belgium NV, submitted pursuant to Article 19 of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the modification of the authoris......Following an application from Barry Callebaut Belgium NV, submitted pursuant to Article 19 of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the modification...... of the authorisation of a health claim related to “cocoa flavanols help maintain the elasticity of blood vessels, which contributes to normal blood flow”, pursuant to Article 13(5) of Regulation (EC) No 1924/2006. The modification concerns an extension of the authorised conditions of use of the claim to a high......-flavanols (HF) cocoa extract to be consumed in capsules, tablets or added to “other foods, including beverages”. Cocoa flavanols, which are the subject of the health claim, have been sufficiently characterised. Maintenance of normal endothelium-dependent vasodilation is a beneficial physiological effect...

Ifrs Versus Cz Gaap: Influence of Construction Contracts on Financial Indicators

Directory of Open Access Journals (Sweden)

Havlová Kristýna

2015-12-01

Full Text Available In 2009, 117 countries all over the world allowed to prepare financial statements according to International Financial Reporting Standards (IFRS including the Czech Republic. Between Czech General Accepted Accounting Principles (CZ GAAP and IFRS are some differences involving reporting of noncurrent fixed assets, leasing, construction contracts etc. Using different accounting procedure we can get different value of assets and assets are one component that is used to calculate the financial performance indicators. Those indicators are then used by investors to assess the financial performance of companies and the application of IFRS instead of CZ GAAP can lead to distinct presentation of enterprise performance. In this paper we examine the influence of construction contract reporting using IFRS and CZ GAAP on financial indicators. IFRS leads to more stable development during the period when the project is in progress. In the last year when the project is finished the figures of both ratios (ROA and Z-score are getting worse which is due to the lower percentage of the construction that is built in this year.

Marketingová strategie společnosti Alarmové systémy CZ

OpenAIRE

Fialová, Petra

2013-01-01

This bachelor thesis deals with the marketing strategy and marketing communications of the company Alarmové systemy CZ and analyses all marketing activities that this company made during its first year of operation in the market. The aim of this thesis is to evaluate the marketing strategy and marketing communications of the company Alarmove systemy CZ and to suggest measures for its improvement. Through analyzing of its marketing communications were discovered the tools that the company uses...

Referentieramingen energie en emissies 2005 - 2020

NARCIS (Netherlands)

Dril AWN van; Elzenga HE; ECN; KMD

2005-01-01

The Reference Projection 2005-2020 covers the future development of Dutch energy use, greenhouse gas emissions and air pollution up to 2020. The Reference projection is based on assumptions regarding economic, structural, technological and policy developments. Two scenarios have been used: the

Rapid detection of genetic modification for GMO monitoring in agriculture

Directory of Open Access Journals (Sweden)

Petrović Sofija

2015-01-01

Full Text Available Transgenic technology has expanded the ways of new genetic variability creation. Genetically modified organisms (GMOs are organisms which total genome is altered in a way that could not happen in nature. GM crops recorded a steady increase in its share in agricultural production. However, for the most part, GMO in agriculture has been limited to two cultivars - soy and corn, and the two genetic modifications, the total herbicide resistance and pest of the Lepidoptera genus. In order to monitor cultivation and trade of GMOs, tests of different precision are used, qualitatively and/or quantitatively determining the presence of genetic modification. Tests for the rapid determination of the presence of GM are suitable, since they can be implemented quickly and accurately, in terms of declared sensitivity, outside or in the laboratory. The example of the use of rapid tests demonstrates their value in use for rapid and efficient monitoring.

Heterologous surface display on lactic acid bacteria: non-GMO alternative?

Science.gov (United States)

Zadravec, Petra; Štrukelj, Borut; Berlec, Aleš

2015-01-01

Lactic acid bacteria (LAB) are food-grade hosts for surface display with potential applications in food and therapy. Alternative approaches to surface display on LAB would avoid the use of recombinant DNA technology and genetically-modified organism (GMO)-related regulatory requirements. Non-covalent surface display of proteins can be achieved by fusing them to various cell-wall binding domains, of which the Lysine motif domain (LysM) is particularly well studied. Fusion proteins have been isolated from recombinant bacteria or from their growth medium and displayed on unmodified bacteria, enabling heterologous surface display. This was demonstrated on non-viable cells devoid of protein content, termed bacteria-like particles, and on various species of genus Lactobacillus. Of the latter, Lactobacillus salivarius ATCC 11741 was recently shown to be particularly amenable for LysM-mediated display. Possible regulatory implications of heterologous surface display are discussed, particularly those relevant for the European Union.

Ontology Matching Across Domains

Science.gov (United States)

2010-05-01

matching include GMO [1], Anchor-Prompt [2], and Similarity Flooding [3]. GMO is an iterative structural matcher, which uses RDF bipartite graphs to...AFRL under contract# FA8750-09-C-0058. References [1] Hu, W., Jian, N., Qu, Y., Wang, Y., “ GMO : a graph matching for ontologies”, in: Proceedings of

EFSA Panel on Biological Hazards (BIOHAZ); Scientific Opinion on the risk of transmission of classical scrapie via in vivo derived embryo transfer in ovine animals

DEFF Research Database (Denmark)

Hald, Tine; Baggesen, Dorte Lau

. Under natural exposure conditions, animals that are heterozygous or homozygous A136R154R171 display respectively a low or negligible risk of being infected. The genetic control of the susceptibility to classical scrapie is also likely to impact on the risk of transmitting the disease via embryo transfer......The risk of transmission of classical scrapie via the transfer of in vivo derived embryo in ovines was assessed, taking into account the scientific information made available since the last EFSA opinion on this topic (2010) (see http://www.efsa.europa.eu/en/efsajournal/pub/1429.htm). The potential...... impact of PrP genotype of the embryo and/or of the ram and donor ewe on this risk was also assessed. The new data made available over the last three years further reinforce the view that classical scrapie could be vertically transmitted in sheep. Since the possibility of such vertical transmission...

Lattice sites and stability of implanted Er in FZ and CZ Si

CERN Document Server

Wahl, U; Langouche, G; Vantomme, A

1998-01-01

We report on the lattice location of $^{167}$Er in Si measured by conversion electron emission channeling. In both FZ and CZ Si, a high fraction of Er (>65%) occupies near-tetrahedral interstitial (T) sites directly following 60 keV room temperature implantation at doses of 6 $\\times 10^{12}$ cm$^{-2}$. For higher doses, the as-implanted near-T fractions of Er visible by emission channeling are smaller, due to the beginning of amorphization. Following the recovery of implantation damage at 600°C, more than 70% of Er is found on near-T sites in both FZ and CZ Si. In FZ Si, Er exhibits a remarkable thermal stability and only prolonged annealing for several hours reduces the near-T fraction. On the other hand, annealing of CZ Si at 900°C for more than 10 minutes results in the majority of Er probes in sites of very low symmetry or disordered surroundings.

EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards), 2013. Scientific Opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed (2013 update)

DEFF Research Database (Denmark)

Licht, Tine Rask; Baggesen, Dorte Lau

EFSA is requested to assess the safety of a broad range of biological agents in the context of notifications for market authorisation as sources of food and feed additives, enzymes and plant protection products. The qualified presumption of safety (QPS) assessment was developed to provide...... a harmonised generic pre-assessment to support safety risk assessments performed by EFSA’s scientific Panels. The safety of unambiguously defined biological agents (at the highest taxonomic unit appropriate for the purpose for which an application is intended), and the completeness of the body of knowledge...... is the one in the most recently published scientific opinion. The 2013 update reviews previously assessed microorganisms including bacteria, yeasts, filamentous fungi, oomycetes and viruses used for plant protection purposes. All taxonomic units previously recommended for the QPS list had their status...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to formulated palm and oat oil emulsion and contribution to the maintenance or achievement of a normal body weight (ID 577) and maintenance of body weight after weight

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to formulated palm and oat oil emulsion and contribution to the maintenance or achievement of a normal body weight and maintenance of body weight after weight loss. The scientific substantiation is based on the information provided by the Member States in the consolidated list...... of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claims is formulated palm and oat oil emulsion. The Panel considers that formulated palm and oat oil emulsion is sufficiently...

Digital Mapping Techniques '05--Workshop Proceedings, Baton Rouge, Louisiana, April 24-27, 2005

Science.gov (United States)

Soller, David R.

2005-01-01

Intorduction: The Digital Mapping Techniques '05 (DMT'05) workshop was attended by more than 100 technical experts from 47 agencies, universities, and private companies, including representatives from 25 state geological surveys (see Appendix A). This workshop was similar in nature to the previous eight meetings, held in Lawrence, Kansas (Soller, 1997), in Champaign, Illinois (Soller, 1998), in Madison, Wisconsin (Soller, 1999), in Lexington, Kentucky (Soller, 2000), in Tuscaloosa, Alabama (Soller, 2001), in Salt Lake City, Utah (Soller, 2002), in Millersville, Pennsylvania (Soller, 2003), and in Portland, Oregon (Soller, 2004). This year's meeting was hosted by the Louisiana Geological Survey, from April 24-27, 2005, on the Louisiana State University campus in Baton Rouge, Louisiana. As in the previous meetings, the objective was to foster informal discussion and exchange of technical information. It is with great pleasure I note that the objective was successfully met, as attendees continued to share and exchange knowledge and information, and to renew friendships and collegial work begun at past DMT workshops. Each DMT workshop has been coordinated by the Association of American State Geologists (AASG) and U.S. Geological Survey (USGS) Data Capture Working Group, which was formed in August 1996, to support the AASG and the USGS in their effort to build a National Geologic Map Database (see Soller and Berg, this volume, and http://ngmdb.usgs.gov/info/standards/datacapt/). The Working Group was formed because increased production efficiencies, standardization, and quality of digital map products were needed for the database?and for the State and Federal geological surveys?to provide more high-quality digital maps to the public. At the 2005 meeting, oral and poster presentations and special discussion sessions emphasized: 1) methods for creating and publishing map products (here, 'publishing' includes Web-based release); 2) field data capture software and

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for chromium

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

parenteral nutrition was considered to be the most convincing, but overall data do not provide sufficient information on the reversibility of the possible deficiencies and the nature of any dose–response curve in order to identify a dietary requirement for humans. The Panel concludes that no Average......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) considered the evidence for setting Dietary Reference Values for chromium. Trivalent chromium (Cr(III)) has been postulated to be necessary for the efficacy of insulin in regulating...... models have not produced consistent results, and that there is no evidence of essentiality of Cr(III) in animal nutrition. Evaluating the possibility of Cr(III) as an essential element for humans, the evidence from reported improvements associated with chromium supplementation in patients on total...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) ; Guidance on the scientific requirements for health claims related to bone, joints, skin and oral health

DEFF Research Database (Denmark)

Tetens, Inge

The Panel on Dietetic Products, Nutrition and Allergies (NDA) has been asked by the European Food Safety Authority (EFSA) to draft guidance on the scientific requirements for health claims related to bone, joints, skin, and oral health. This guidance has been drawn from scientific opinions...

Process research of non-CZ silicon material

Science.gov (United States)

Campbell, R. B.

1984-01-01

Advanced processing techniques for non-CZ silicon sheet material that might improve the cost effectiveness of photovoltaic module production were investigated. Specifically, the simultaneous diffusion of liquid boron and liquid phosphorus organometallic precursors into n-type dendritic silicon web was examined. The simultaneous junction formation method for solar cells was compared with the sequential junction formation method. The electrical resistivity of the n-n and p-n junctions was discussed. Further research activities for this program along with a program documentation schedule are given.

Emotional attitudes of young people completing secondary schools towards genetic modification of organisms (GMO) and genetically modified foods (GMF).

Science.gov (United States)

Jurkiewicz, Anna; Zagórski, Jerzy; Bujak, Franciszek; Lachowski, Stanisław; Florek-Łuszczki, Magdalena

2014-01-01

The objective of the study was recognition of the opinions of adolescents completing secondary schools concerning genetically modified organisms and genetically modified food, especially the respondents' emotional attitude towards scientific achievements in the area of live genetically modified organisms. The study covered a group of 500 school adolescents completing secondary school at the level of maturity examination. The study was conducted by the method of a diagnostic survey using a self-designed questionnaire form. Knowledge concerning the possible health effects of consumption of food containing GMO among adolescents competing secondary schools is on a relatively low level; the adolescents examined 'know rather little' or 'very little know' about this problem. In respondents' opinions the results of reliable studies pertaining to the health effects of consumption of GMO 'rather do not exist'. The respondents are against the cultivation of GM plants and breeding of GM animals on own farm in the future. Secondary school adolescents considered that the production of genetically modified food means primarily the enrichment of biotechnological companies, higher income for food producers, and not the elimination of hunger in the world or elimination of many diseases haunting humans.

Sociolinguistic bibliography of European Countries for 2005: CZ

Czech Academy of Sciences Publication Activity Database

Kaderka, Petr

2007-01-01

Roč. 21, - (2007), s. 206-212 ISSN 0933-1883 R&D Projects: GA ČR GA405/06/1468 Institutional research plan: CEZ:AV0Z90610518 Keywords : sociolinguistics * bibliography * Czech Republic Subject RIV: AI - Linguistics

Is the titer of adipokinetic peptides in Leptinotarsa decemlineata fed on genetically modified potatoes increased by oxidative stress?

Czech Academy of Sciences Publication Activity Database

Kodrík, Dalibor; Krishnan, Natraj; Habuštová, Oxana

2007-01-01

Roč. 28, č. 5, (2007), s. 974-980 ISSN 0196-9781 R&D Projects: GA ČR GA522/05/0151; GA ČR(CZ) GA522/06/1591 Institutional research plan: CEZ:AV0Z50070508 Keywords : adipokinetic hormone * oxidative stress * GMO Subject RIV: ED - Physiology Impact factor: 2.368, year: 2007

EFSA NDA Panel (EFSA Panel on Panel on Dietetic Products Nutrition and Allergies), 2014. Scientific Opinion on Dietary Reference Values for iodine

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived Dietary Reference Values (DRVs) for iodine, which are provided as Adequate Intake (AI). Iodine is essential for the synthesis of thyroid hormones. Through these hormones, iodine...... stores in conditions of adequate iodine status before pregnancy and considers that a full compensation for the iodine secreted in breast milk is not justified for the derivation of an AI for iodine for lactating women....

Electrochemical sensors based on stationary electrodes and immobilized DNA or its fragments and the assessment of their analytical potentials

Czech Academy of Sciences Publication Activity Database

Babkina, S. S.; Paleček, Emil; Jelen, František; Fojta, Miroslav

2005-01-01

Roč. 60, č. 6 (2005), s. 567-572 ISSN 1061-9348. [VII All-Russia Conference (with international participation) on Electrochemical Methods of Analysis. Ufa, 23.05.2004-27.05.2004] R&D Projects: GA MPO(CZ) 1H-PK/42 Institutional research plan: CEZ:AV0Z50040507 Keywords : electrochemical biosensor * DNA imobilization * nitrocellulose matrix Subject RIV: BO - Biophysics Impact factor: 0.496, year: 2005

Epilithic algae from caves of the Krakowsko-Częstochowska Upland (Southern Poland

Directory of Open Access Journals (Sweden)

Joanna Czerwik-Marcinkowska

2011-01-01

Full Text Available This paper describes the first study of algae assemblages in 20 caves in the Krakowsko-Częstochowska Upland (Southern Poland, in the period between 2005-2006. The investigations showed mostly on epilithic algae and their subaeric habitats (rock faces within caves and walls at cave entrances. The morphological and cytological variability of algae were studied in fresh samples, in cultures grown on agar plates and in SPURR preparations. A total of 43 algae species was identified, mostly epilithic species and tolerant of low light intensities. The largest group was formed by representatives of the division Chlorophyta (24 species, and then the division Chrysophyta (Heterokontophyta - 17 species, with 9 species belonging to the class Bacillariophyceae, 7 species - Xanthophyceae and 1 species representing the class Eustigmatophyceae. Dinophyta (2 species constituted the last and the smallest group. Among the collected algae, the following species deserve special attention: Thelesphaera alpina, Bracteacoccus minor, Trachychloron simplex, Tetracystis intermedia and T. cf. isobilateralis. The last species was not earlier found in Europe. Identification of species was greatly aided by examination of cell ultrastructure, which provided an array of further features, increasing chances of correct species identification. Furthermore, the studies focused that algae, although usually remaining under dominance of cyanobacteria, excellently differentiate this special area and even enrich it.

[Comparison of the present and previously used protocol of risk stratification in children with acute lymphoblastic leukemia].

Science.gov (United States)

Glodkowska, Eliza; Bialas, Agnieszka; Jackowska, Teresa

2007-01-01

Acute lymphoblastic leukaemia (ALL) is one of the most common cancers in children. In Poland, since November 2002 a new protocol of risk stratification has been recommended for assessment of risk factors and for choosing therapy regimens. assessment of accuracy of protocol ALL-IC 2002 in comparison to previously used risk stratification protocols. ALL was diagnosed in 100 children (44 girls, 56 boys; 1-18 years of age) in the Department of Pediatric Hematology and Oncology, Warsaw Medical University, over the period from November 2002 to November 2006. According to the ALL-IC 2002 protocol the patients were divided into three risk groups: SR-standard, IR-intermediate and HR-high. The stratification was by age, leukocyte count, cytogenetic changes, early response to prednisone therapy and bone marrow remission. In the previously used risk stratification protocols-BFM-90, only hepatosplenomegaly and the number of blasts in peripheral blood (PB) were considered, and the patients were divided into three risk groups: low (LRG1.2). out of the 100 patients qualified for treatment regimens according to the ALL-IC 2002 protocol, 97 entered remission, 11 died and 3 had a relapse. Under the ALL-IC 2002 protocol these children were stratified into the following groups: SR-31%, IR-44% and HR-25%. In the previously used stratification, there would be 26% children in low, 46% in the medium and 28% in the high risk group. According to the BFM-90 protocol 18/31 (58%) and 16/44 (36%) patients from the SR and IR groups respectively would be given more intensive treatment. On the other hand 11/44 (25%) and 14/25 (56%) patients from the IR and HR groups respectively would be given less intensive treatment. 1. ALL-IC 2002 protocol in comparison with the previously used protocol BFM-90, changes the qualification of children with ALL for the SR, IR and HR risk groups. This is linked to basic change of treatment protocol, adequate to severity of disease. 2. Children with ALL qualified

Developing a good practice for the review of evidence relevant to GMO risk assessment

DEFF Research Database (Denmark)

Kohl, Christian; Craig, Wendy; Frampton, Geoff

2013-01-01

such research results in a transparent, reproducible and unbiased manner. The EU-funded project GRACE (GMO Risk Assessment and Communication of Evidence) aims to explore, adapt, apply, and promote these tools to enhance accountability in decision-making by providing policy makers with comprehensive, science......-based information on potential impacts (scenarios) of genetically modified plants and their derived products. Furthermore, GRACE will establish an open-access database in order to support the review process, to mirror the evidence synthesis, to assist the dissemination of results and conclusions, as well as to act......, in more general terms, as an information resource on biosafety of genetically modified plants....

Energy consumption 2005 with Danish industry

International Nuclear Information System (INIS)

Anon.

2006-01-01

The energy consumption in the Danish industries decreased with 4% from 2003 to 2005. The consumption of liquid fuels and district heat decreased with 27% and 21%, respectively. The consumption of solid fuels increased with 13%. The aim of the statistics is to elucidate the industry's energy consumption and its composition. The statistics present the development in the industry from 1973 to 2005, in which period the composition of the energy consumption has changed significantly. Especially, consumption of liquid fuels has decreased and consumption of gas and electricity has increased. (ln)

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to acetic acid and maintenance of normal blood pressure (ID 1447) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

that maintenance of normal blood pressure is a beneficial physiological effect. In weighing the evidence, the Panel took into account that although one animal study showed an effect of acetic acid administration on systolic blood pressure, results from two human intervention studies are conflicting...... claims in relation to acetic acid and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders....... The food that is the subject of the health claim is apple vinegar drink. From the references provided for the scientific substantiation of the claim, the Panel assumes that the food constituent which is responsible for the claimed effect is acetic acid. The Panel considers that acetic acid is sufficiently...

EFSA CONTAM Panel (EFSA Panel on Contaminants in the Food Chain), 2015. Scientific Opinion on risks for public health related to the presence of chlorate in food

DEFF Research Database (Denmark)

Petersen, Annette

Following a request from the European Commission, the risks to human health related to the presence of chlorate in food were assessed by the EFSA Panel on Contaminants in the Food Chain (CONTAM Panel). The presence of chlorate in food can arise from the use of chlorinated water for food processing...... and the disinfection of food-processing equipment. Inhibition of iodine uptake in humans was identified as the critical effect for chronic exposure to chlorate. A tolerable daily intake (TDI) of 3 µg chlorate/kg body weight (b.w.) was set by read-across from a TDI of 0.3 µg/kg b.w. derived for this effect...... with mild or moderate iodine deficiency. Mean and 95th percentile acute exposures were below the ARfD for all age groups indicating no concern. Based on the current practices in food industry, application of a hypothetical maximum residue limit (MRL) of 0.7 mg/kg for all foodstuffs and drinking water would...

End-of-year-closure 2004/2005

CERN Multimedia

Human Resources Department

2004-01-01

As announced in Weekly Bulletin Nº 3/2004, the Laboratory will be closed from Saturday 18 December 2004 to Sunday 2 January 2005 inclusive. This period consists of 16 days: 4 days' official holiday, i.e. 24, 25 and 31 December 2004 and 1st January 2005; 6 days' special paid leave in accordance with Article R II 4.34 of the Staff Regulations, i.e. 20, 21, 22, 27, 28, and 29 December 2004; 1 Saturday, i.e. 18 December 2004; 2 days, 23 December 2004 to compensate for 25 December 2004 and 30 December 2004 to compensate for 1st January 2005 (Article R II 4.33 of the Staff Regulations); 3 Sundays, i.e. 19 and 26 December 2004 and 2 January 2005. The first working day in the New Year will be Monday 3 January 2005. Further information will be available from Department Secretariats, specifically concerning the conditions applicable to members of the personnel who are required to work during this period. Human Resources Department Tel. 74474

Making the most of CZ seismics: Improving shallow critical zone characterization using surface-wave analysis

Science.gov (United States)

Pasquet, S.; Wang, W.; Holbrook, W. S.; Bodet, L.; Carr, B.; Flinchum, B. A.

2017-12-01

Estimating porosity and saturation in the shallow subsurface over large lateral scales is vitally important for understanding the development and evolution of the Critical Zone (CZ). Because elastic properties (P- and S-wave velocities) are particularly sensitive to porosity and saturation, seismic methods (in combination with petrophysical models) are effective tools for mapping CZ architecture and processes. While many studies employ P-wave refraction methods, fewer use the surface waves that are typically also recorded in those same surveys. Here we show the value of exploiting surface waves to extract supplementary shear-wave velocity (Vs) information in the CZ. We use a new, user-friendly, open-source MATLAB-based package (SWIP) to invert surface-wave data and estimate lateral variations of Vs in the CZ. Results from synthetics show that this approach enables the resolution of physical property variations in the upper 10-15 m below the surface with lateral scales of about 5 m - a vast improvement compared to P-wave tomography alone. A field example at a Yellowstone hydrothermal system also demonstrates the benefits of including Vs in the petrophysical models to estimate not only porosity but also saturation, thus highlighting subsurface gas pathways. In light of these results, we strongly suggest that surface-wave analysis should become a standard approach in CZ seismic surveys.

Craft's brand equity in CZ, focusing on canoeing

OpenAIRE

Šťastný, Šimon

2013-01-01

Title: Craft's brand equity in CZ, focusing on flat water canoeing. Objectives: The objective of this dissertation is to find out what is the Craft's brand equity and to suggest how to improve it on the Czech market. That would be based on marketing research and supplementary interview. I will be using marketing research and supplementary interview. Methods: In this dissertation the method of electronic questioning was used. The questionnaires were sent by email address after first contact on...

The Lyman alpha reference sample VII. Spatially resolved H alpha kinematics

Czech Academy of Sciences Publication Activity Database

Herenz, E.Ch.; Gruyters, P.; Orlitová, Ivana; Hayes, M.; Ostlin, G.; Cannon, J.M.; Roth, M.M.; Bik, A.; Pardy, S.; Oti-Floranes, H.; Mas-Hesse, J. M.; Adamo, A.; Atek, H.; Duval, F.; Guaita, L.; Kunth, D.; Laursen, P.; Melinder, J.; Puschnig, J.; Rivera-Thorsen, T.; Schaerer, D.; Verhamme, A.

2016-01-01

Roč. 587, March (2016), A78/1-A78/27 ISSN 0004-6361 R&D Projects: GA ČR(CZ) GP14-20666P Institutional support: RVO:67985815 Keywords : galaxies * ISM * starburst Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 4.378, year: 2014

Emotional attitudes of young people completing secondary schools towards genetic modification of organisms (GMO and genetically modified foods (GMF

Directory of Open Access Journals (Sweden)

Anna Jurkiewicz

2014-03-01

Full Text Available Objective. The objective of the study was recognition of the opinions of adolescents completing secondary schools concerning genetically modified organisms and genetically modified food, especially the respondents’ emotional attitude towards scientific achievements in the area of live genetically modified organisms. Material and method. The study covered a group of 500 school adolescents completing secondary school at the level of maturity examination. The study was conducted by the method of a diagnostic survey using a self-designed questionnaire form. Results. Knowledge concerning the possible health effects of consumption of food containing GMO among adolescents competing secondary schools is on a relatively low level; the adolescents examined ‘know rather little’ or ‘very little know’ about this problem. In respondents’ opinions the results of reliable studies pertaining to the health effects of consumption of GMO ‘rather do not exist’. The respondents are against the cultivation of GM plants and breeding of GM animals on own farm in the future. Secondary school adolescents considered that the production of genetically modified food means primarily the enrichment of biotechnological companies, higher income for food producers, and not the elimination of hunger in the world or elimination of many diseases haunting humans.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to foods with reduced lactose content and decreasing gastro-intestinal discomfort caused by lactose intake in lactose intolerant individuals (ID 646, 1224, 1238, 1339

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to foods with reduced lactose content and decreasing gastro-intestinal discomfort caused by lactose intake in lactose intolerant individuals. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims...... and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claim is lactose, which should be “decreased” or “reduced” in foods in order to obtain the claimed effect. The Panel considers that lactose is sufficiently characterised....... The claimed effects are “lactose intolerance”, “decrease lactose malabsorption symptoms” and “lactose digestion”. The target population is assumed to be lactose intolerant individuals. The Panel assumes that the claimed effects refer to decreasing gastro-intestinal discomfort caused by lactose intake...

Dendrochronological evidence of cockchafer (Melolontha sp.) outbreaks in subfossil tree-trunks from Tovačov (CZ Moravia)

Czech Academy of Sciences Publication Activity Database

Kolář, Tomáš; Rybníček, M.; Tegel, W.

2013-01-01

Roč. 31, č. 1 (2013), s. 29-33 ISSN 1125-7865 R&D Projects: GA MŽP(CZ) SP/2D1/93/07; GA MŠk(CZ) ED1.1.00/02.0073; GA MŠk(CZ) EE2.3.20.0256 Institutional support: RVO:67179843 Keywords : Cockchafer * Oak * Tree-ring * Dendrochronology * Subfossil trunk Subject RIV: EH - Ecology, Behaviour Impact factor: 1.697, year: 2013 http://www.sciencedirect.com/science/article/pii/S1125786512000574

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and gamma-linolenic acid (GLA) and contribution to normal cognitive function (ID 532) and maintenance

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA) and gamma-linolenic acid (GLA) and contribution to normal cognitive function and maintenance of normal bone. The scientific substantiation is based on the information provided by the Member States in the consolidated list...... and fish oil”. From the references provided, the Panel assumes that the food constituents that are the subject of the claims are the n-6 fatty acid gamma-linolenic acid (GLA) in evening primrose oil and the n-3 long-chain polyunsaturated fatty acids docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA...... of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituents that are the subjects of the health claims are “omega-3 and omega-6 fatty acids (GLA)”, “gamma-linolenic acid + eicosapentaenoic acid (GLA+EPA)”, and “evening primrose oil...

A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples.

Science.gov (United States)

Zhu, Pengyu; Wang, Chenguang; Huang, Kunlun; Luo, Yunbo; Xu, Wentao

2016-03-18

Digital polymerase chain reaction (PCR) has developed rapidly since it was first reported in the 1990s. However, pretreatments are often required during preparation for digital PCR, which can increase operation error. The single-plex amplification of both the target and reference genes may cause uncertainties due to the different reaction volumes and the matrix effect. In the current study, a quantitative detection system based on the pretreatment-free duplex chamber digital PCR was developed. The dynamic range, limit of quantitation (LOQ), sensitivity and specificity were evaluated taking the GA21 event as the experimental object. Moreover, to determine the factors that may influence the stability of the duplex system, we evaluated whether the pretreatments, the primary and secondary structures of the probes and the SNP effect influence the detection. The results showed that the LOQ was 0.5% and the sensitivity was 0.1%. We also found that genome digestion and single nucleotide polymorphism (SNP) sites affect the detection results, whereas the unspecific hybridization within different probes had little side effect. This indicated that the detection system was suited for both chamber-based and droplet-based digital PCR. In conclusion, we have provided a simple and flexible way of achieving absolute quantitation for genetically modified organism (GMO) genome samples using commercial digital PCR detection systems.

A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples

Directory of Open Access Journals (Sweden)

Pengyu Zhu

2016-03-01

Full Text Available Digital polymerase chain reaction (PCR has developed rapidly since it was first reported in the 1990s. However, pretreatments are often required during preparation for digital PCR, which can increase operation error. The single-plex amplification of both the target and reference genes may cause uncertainties due to the different reaction volumes and the matrix effect. In the current study, a quantitative detection system based on the pretreatment-free duplex chamber digital PCR was developed. The dynamic range, limit of quantitation (LOQ, sensitivity and specificity were evaluated taking the GA21 event as the experimental object. Moreover, to determine the factors that may influence the stability of the duplex system, we evaluated whether the pretreatments, the primary and secondary structures of the probes and the SNP effect influence the detection. The results showed that the LOQ was 0.5% and the sensitivity was 0.1%. We also found that genome digestion and single nucleotide polymorphism (SNP sites affect the detection results, whereas the unspecific hybridization within different probes had little side effect. This indicated that the detection system was suited for both chamber-based and droplet-based digital PCR. In conclusion, we have provided a simple and flexible way of achieving absolute quantitation for genetically modified organism (GMO genome samples using commercial digital PCR detection systems.

Vliv sociálních medií na společnost Answear cz.

OpenAIRE

Zuková, Nela

2017-01-01

The dissertation Social Media effect on Answear CZ company focuses on marketing and on-line marketing issues and social media potential in terms of business performance and marketing awareness. In the theoretical part, a marketing and on-line marketing will be defined and described, followed by social media and possibilities of their application in business, such as Facebook, Instagram, Twitter and others. In practical part, a social media strategy of Answear CZ company will be presented, dis...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to lactose and increase in calcium absorption leading to an increase in calcium retention (ID 668) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to lactose and increase in calcium absorption leading to an increase in calcium retention. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member...... States or directly from stakeholders. The food constituent that is the subject of the health claim is lactose. The Panel considers that lactose is sufficiently characterised. The claimed effect is “calcium absorption”. The target population is assumed to be the general population. The Panel notes...... between the consumption of lactose and an increase in calcium absorption leading to an increase in calcium retention....

The community-driven BiG CZ software system for integration and analysis of bio- and geoscience data in the critical zone

Science.gov (United States)

Aufdenkampe, A. K.; Mayorga, E.; Horsburgh, J. S.; Lehnert, K. A.; Zaslavsky, I.; Valentine, D. W., Jr.; Richard, S. M.; Cheetham, R.; Meyer, F.; Henry, C.; Berg-Cross, G.; Packman, A. I.; Aronson, E. L.

2014-12-01

Here we present the prototypes of a new scientific software system designed around the new Observations Data Model version 2.0 (ODM2, https://github.com/UCHIC/ODM2) to substantially enhance integration of biological and Geological (BiG) data for Critical Zone (CZ) science. The CZ science community takes as its charge the effort to integrate theory, models and data from the multitude of disciplines collectively studying processes on the Earth's surface. The central scientific challenge of the CZ science community is to develop a "grand unifying theory" of the critical zone through a theory-model-data fusion approach, for which the key missing need is a cyberinfrastructure for seamless 4D visual exploration of the integrated knowledge (data, model outputs and interpolations) from all the bio and geoscience disciplines relevant to critical zone structure and function, similar to today's ability to easily explore historical satellite imagery and photographs of the earth's surface using Google Earth. This project takes the first "BiG" steps toward answering that need. The overall goal of this project is to co-develop with the CZ science and broader community, including natural resource managers and stakeholders, a web-based integration and visualization environment for joint analysis of cross-scale bio and geoscience processes in the critical zone (BiG CZ), spanning experimental and observational designs. We will: (1) Engage the CZ and broader community to co-develop and deploy the BiG CZ software stack; (2) Develop the BiG CZ Portal web application for intuitive, high-performance map-based discovery, visualization, access and publication of data by scientists, resource managers, educators and the general public; (3) Develop the BiG CZ Toolbox to enable cyber-savvy CZ scientists to access BiG CZ Application Programming Interfaces (APIs); and (4) Develop the BiG CZ Central software stack to bridge data systems developed for multiple critical zone domains into a single

DML-CZ : the experience of a medium-sized Digital Mathematics Library

Czech Academy of Sciences Publication Activity Database

Bartošek, M.; Rákosník, Jiří

2013-01-01

Roč. 60, č. 8 (2013), s. 1028-1033 ISSN 0002-9920 Institutional support: RVO:67985840 Keywords : Czech Digital Mathematics Library * DML-CZ * digital libraries Subject RIV: BA - General Mathematics http://www.ams.org/notices/201308/rnoti-p1028.pdf

22 CFR 401.27 - Notice and publication.

Science.gov (United States)

2010-04-01

... 22 Foreign Relations 2 2010-04-01 2010-04-01 true Notice and publication. 401.27 Section 401.27 Foreign Relations INTERNATIONAL JOINT COMMISSION, UNITED STATES AND CANADA RULES OF PROCEDURE References § 401.27 Notice and publication. (a) The secretary to whom a reference is presented shall receive and...

Beverage Consumption Habits in Italian Population: Association with Total Water Intake and Energy Intake

Directory of Open Access Journals (Sweden)

Lorenza Mistura

2016-10-01

Full Text Available Background: The aim of this study was to investigate total water intake (TWI from water, beverages and foods among Italian adults and the elderly. Methods: Data of 2607 adults and the elderly, aged 18–75 years from the last national food consumption survey, INRAN-SCAI 2005-06, were used to evaluate the TWI. The INRAN-SCAI 2005-06 survey was conducted on a representative sample of 3323 individuals aged 0.1 to 97.7 years. A 3-day semi-structured diary was used for participants to record the consumption of all foods, beverages and nutritional supplements. Results: On average, TWI was 1.8 L for men and 1.7 L for women. More than 75% of women and 90% of men did not comply with the European Food Safety Authority (EFSA Adequate Intake. The contribution of beverages to the total energy intake (EI was 6% for the total sample. Water was the most consumed beverage, followed by alcoholic beverages for men and hot beverages for women. Conclusion: According to the present results, adults and elderly Italians do not reach the adequate intake for water as suggested by the EFSA and by the national reference level of nutrient and energy intake. Data on water consumption should also be analyzed in single socio-demographic groups in order to identify sub-groups of the population that need more attention and to plan more targeted interventions.

The convergence dynamics of a transition economy: the case of the Czech Republic

Czech Academy of Sciences Publication Activity Database

Brůha, J.; Podpiera, Jiří; Polák, S.

2010-01-01

Roč. 27, č. 1 (2010), s. 116-124 ISSN 0264-9993 Grant - others:Česká národní banka(CZ) E4/2005 Institutional research plan: CEZ:AV0Z70850503 Keywords : economic transition * country modeling * convergence Subject RIV: AH - Economics Impact factor: 0.601, year: 2010

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to medium-chain triglycerides and reduction in body weight (ID 643, 677, 1614) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to medium-chain triglycerides and reduction in body weight. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...... stakeholders. The food constituent that is the subject of the health claims is medium-chain triglycerides. In the context of the references provided, the Panel assumes that the food constituent which is the subject of the health claims is medium-chain fatty acids, which should replace long-chain fatty acids...... in triglycerides in order to obtain the claimed effect. The Panel considers that the food constituent, medium-chain fatty acids, which is the subject of the health claims, is sufficiently characterised in relation to the claimed effect. The claimed effect is “weight management”. The target population is assumed...

Model Predictive Control for Offset-Free Reference Tracking

Czech Academy of Sciences Publication Activity Database

Belda, Květoslav

2016-01-01

Roč. 5, č. 1 (2016), s. 8-13 ISSN 1805-3386 Institutional support: RVO:67985556 Keywords : offset-free reference tracking * predictive control * ARX model * state-space model * multi-input multi-output system * robotic system * mechatronic system Subject RIV: BC - Control Systems Theory http://library.utia.cas.cz/separaty/2016/AS/belda-0458355.pdf

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to carbonate and bicarbonate salts of sodium and potassium and maintenance of normal bone (ID 331, 1402) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to carbonate and bicarbonate salts of sodium and potassium and maintenance of normal bone. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member...... States or directly from stakeholders. The food constituents that are the subject of the health claim are carbonate and bicarbonate salts of sodium and potassium. The Panel considers that carbonate and bicarbonate salts of sodium and potassium are sufficiently characterised. The claimed effects are “acid...... the dietary intake of carbonate or bicarbonate salts of sodium or potassium and maintenance of normal bone....

The 2005 European e-Business Readiness Index

OpenAIRE

Pennoni, Fulvia; Tarantola, Stefano; Latvala, Ari

2006-01-01

Assessment of the eEurope 2005 Action Plan Benchmarking Index “E-Business Readiness Composite Indicator” using data collected by National Statistical Institutes and harmonised by Eurostat, using surveys “ICT usage of enterprises”, with reference years 2003 and 2004. This report contains data from 26 countries as collected in 2004 and as reported by Eurostat in June 2005. Performed analyses include obustness analysis, uncertainty and sensitivity analysis for two categories of ICT (Adoption ...

Overview and charge - Snowmass Workshop 2005

International Nuclear Information System (INIS)

Berger, E. L.

2006-01-01

This contribution to the published Proceedings records the opening talk I presented on the first morning of the 2005 International Linear Collider Workshop in Snowmass, CO, August 14 - 27, 2005. It includes a summary of the motivation for the workshop, the scientific goals and charges for the working groups, the initial plans of the accelerator, detector, and physics groups, and the activities of the communication, education, and outreach group. This document also describes organizational aspects of the meeting, particularly the scientific committee structure, the self-organization of the working groups, the composition of the indispensable secretariat and computer support teams, and the sources of funding support. The report serves as an introduction to the proceedings whose individual papers and summary documents must be consulted for an appreciation of the accomplishments and progress made at Snowmass in 2005 toward the realization of an International Linear Collider

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to “αS1-casein tryptic hydrolysate” and alleviation of psychological stress (ID 656, 1819, 4253) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to “αS1-casein tryptic hydrolysate” and alleviation of psychological stress. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... that alleviation of psychological stress might be a beneficial physiological effect. No references were provided from which conclusions could be drawn for the scientific substantiation of the claim. On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on health benefits of seafood (fish and shellfish) consumption in relation to health risks associated with exposure to methylmercury

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following a request from the European Commission to address the risks and benefits as regards fish/seafood consumption related to relevant beneficial substances (e.g. nutrients such as n-3 long-chain polyunsaturated fatty acids) and the contaminant methylmercury, the Panel on Dietetic Products...... effects of seafood consumption in relation to health outcomes and population subgroups that have been identified by the FAO/WHO Joint Expert Consultation on the Risks and Benefits of Fish Consumption and/or the EFSA Panel on Contaminants in the context of a risk assessment related to the presence...... of mercury and methylmercury in food as relevant for the assessment. These included the effects of seafood consumption during pregnancy on functional outcomes of children’s neurodevelopment and the effects of seafood consumption on cardiovascular disease risk in adults. The Panel concluded that consumption...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies) , 2013. Scientific Opinion on the safety of “ coriander seed oil ” as a Novel Food ingredient

DEFF Research Database (Denmark)

Tetens, Inge; Poulsen, Morten

were observed when feeding other vegetable oils, although not as severe as that seen for CSO. The dose level of CSO was more than a thousand fold higher than the proposed use level. In a subchronic study using 150, 450 or 1 000 mg/kg bw per day of CSO, a treatment-related effect was observed on blood......Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on “coriander seed oil (CSO)” as a novel food ingredient (NFI) in the context of Regulation (EC) No 258/97. Petroselinic acid (PA......) is the major fatty acid in CSO. Conventional edible oil technologies are used to manufacture the NFI. The NFI is intended to be marketed as a food supplement for healthy adults, at a maximum level of 600 mg per day (i.e. 8.6 mg/kg bw per day for a 70 kg person), which would lead to significantly higher intakes...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sodium bicarbonate and maintenance of normal blood pressure (ID 1404) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to sodium bicarbonate and maintenance of normal blood pressure. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...... stakeholders. The food constituent that is the subject of the health claim is sodium bicarbonate. The Panel considers that sodium bicarbonate is sufficiently characterised. The claimed effect is “blood pressure reduction”. The target population is assumed to be the general population. In the context...... substantiation of the claim. On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the consumption of sodium bicarbonate and maintenance of normal blood pressure....

[Bibliometric study of the journal Nutrición Hospitalaria for the period 2001--2005: Part 2, consumption analysis; the bibliographic references].

Science.gov (United States)

Castera, V T; Sanz Valero, J; Juan-Quilis, V; Wanden-Berghe, C; Culebras, J M; García de Lorenzo y Mateos, A

2008-01-01

To describe and assess the consumption of the information consulted and cited in the articles published in the journal Nutrición Hospitalaria for the period 2001--2005 by means of bibliometric analysis. Cross-sectional descriptive analysis of the results obtained from the analysis of the lists of bibliographic references of the articles published at Nutrición Hospitalaria. We studied the most cited journals, the signatures index, the type of document referred, the publication language, the distribution of geographical origin, and obsolescence and readiness index. We took into account all types of documents with the exception of Communications to Congresses. 345 articles were published at Nutr Hosp, containing 8,113 bibliographic references, with a median of 18, a maximum of 136 and minimum of 0 BR per article. The mean (rate of publications per published article during the specified period) is 23.52 (95% IC 20.93-26.10) and the mean at 5% is 20.66 per article. The 25th and 75th percentiles are 6 and 32, respectively, the interquartile interval being 26 BR per document. The semi-period of Burton and Kebler is 7 years and the Price Index is 38.18%. The bibliographic references, the consumption of information, of the articles published at Nutrición Hospitalaria present parameters similar to other journals on health science. However, good data on obsolescence are observed, which reveal the good validity of most of the references studied.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to anthocyanins from Ribes nigrum L. and improvement of visual adaptation to the dark (ID 2750) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to anthocyanins from Ribes nigrum L. and improvement of visual adaptation to the dark. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States...... or directly from stakeholders. The food constituent that is the subject of the health claim is anthocyanins from Ribes nigrum L. The Panel considers that anthocyanins from Ribes nigrum L. are sufficiently characterised. The claimed effect is “eye health”. The target population is assumed to be the general...... population. In the context of the proposed wordings, the Panel assumes that the claimed effect refers to an improvement of visual adaptation to the dark. The Panel considers that improvement of visual adaptation to the dark is a beneficial physiological effect. No human studies were provided from which...

Marketingová strategie elektronického obchodu Fotodekorace.cz

OpenAIRE

Vlčková, Simona

2011-01-01

The thesis is analysing methods of increasing the visit rate of the internet shop fotodekorace.cz, especially it is concerned about the utilities of the internet marketing. The aim of the master's thesis is, on the basis of undertaken analysis suggest internet marketing strategy of this particular internet project, which should lead to an increase in sales. The premises of the thesis is to analyse the effectively of online marketing activities. One of the outcomes is the outline of the effect...

Zoonoses in Europe: distribution and trends - the EFSA-ECDC Community Summary Report 2008

DEFF Research Database (Denmark)

Lahuerta, Angela; Helwigh, Birgitte; Mäkelä, Pia

2010-01-01

,554) in the EU. Foodstuffs that are considered the main source for human listeriosis in the EU include ready-to-eat (RTE) products (fish and meat), soft cheeses, salads and sandwiches. An EFSA-ECDC collaborative survey on Listeria in RTE products and in clinical cases of human listeriosis started in January 2010......, the results of which will contribute to a better understanding about listeriosis in the EU. Q-fever increased by 172% in 2008 (1,594) compared with 2007 (585). This was mainly due to several outbreaks in people entering areas with infected sheep and goats mainly in the Netherlands. In-depth investigations...... from 2007 (2,905 cases). In animals VTEC was mainly isolated from cattle and, in lower proportion from small ruminants such as sheep and goats. In food, VTEC was detected in a considerable proportion of cow milk samples. The 2008 annual Community Summary Report describes the five-year trends...

Antimicrobial resistence of Shigella species isolated during 2004 and 2005 from selected sites in Zimbabwe.

Science.gov (United States)

Ndlovu, N; Tarupiwa, A; Mudzori, J T

2006-01-01

To determine the predominant serotype and antibiotic sensitivity pattern of Shigella isolates during 2004 and 2005 in Zimbabwe. Cross sectional study. National Microbiology Reference Laboratory (NMRL), Harare, Zimbabwe. 259 clinical isolates of Shigella species isolated during 2004 and 2005 in Zimbabwe were studied. These samples had been referred to the NMRL for further testing. Serotype and antibiotic sensitivity pattern of Shigella species. Of the 259 clinical isolates of Shigella tested the following species were serotyped; 141 (54.4%) were S. flexneri; 70 (27%) S. sonnei; 38 (14.7%) S. dysenteriae and 10 (3.9%) S. boydii. About 4% of all Shigella isolates tested showed full sensitivity to commonly used antibiotics, 20.8% were resistant to one antibiotic only while 75.3% were resistant to at least two antibiotics. The most common resistance among Shigella species was to cotrimoxazole (89%), tetracycline (73%), ampicillin (49%) and chloramphenicol (41%). High susceptibility among Shigella species was observed to nalidixic acid (86%), ciprofloxacin (99%) and ceftazidine (99%). There was a low drug resistance of Shigella species to nalidixic acid, a drug of choice in Zimbabwe, except among Shigella dysenteriae type 1 strains. Continuous monitoring of the susceptibility patterns of Shigella species is important in order to detect the emergence of drug resistance and to update guidelines for antibiotic treatment in shigellosis.

Novel GMO-Based Vaccines against Tuberculosis: State of the Art and Biosafety Considerations.

Science.gov (United States)

Leunda, Amaya; Baldo, Aline; Goossens, Martine; Huygen, Kris; Herman, Philippe; Romano, Marta

2014-06-16

Novel efficient vaccines are needed to control tuberculosis (TB), a major cause of morbidity and mortality worldwide. Several TB vaccine candidates are currently in clinical and preclinical development. They fall into two categories, the one of candidates designed as a replacement of the Bacille Calmette Guérin (BCG) to be administered to infants and the one of sub-unit vaccines designed as booster vaccines. The latter are designed as vaccines that will be administered to individuals already vaccinated with BCG (or in the future with a BCG replacement vaccine). In this review we provide up to date information on novel tuberculosis (TB) vaccines in development focusing on the risk assessment of candidates composed of genetically modified organisms (GMO) which are currently evaluated in clinical trials. Indeed, these vaccines administered to volunteers raise biosafety concerns with respect to human health and the environment that need to be assessed and managed.

Volcanic Ash fall Impact on Vegetation, Colima 2005

Science.gov (United States)

Garcia, M. G.; Martin, A.; Fonseca, R.; Nieto, A.; Radillo, R.; Armienta, M.

2007-05-01

An ash sampling network was established arround Colima Volcano in 2005. Ash fall was sampled on the North, Northeast, East, Southeast, South, Southwest and West of the volcano. Samples were analyzed for ash components, geochemistry and leachates. Ash fall ocurred on April (12), May (10, 23), June (2, 6, 9, 10, 12, 14), July (27), September (27), October (23) and November (24). Most of the ash is made of andesitic dome-lithics but shows diferences in crystal, juvenile material and lithic content. In May, some samples contained grey and dark pumice (scoria). Texture varies from phi >4 to phi 0. Leachate concentration were low: SO4 (7.33-54.19) Cl- (2.29-4.97) and F- (0.16-0.37). During 2005, Colima Volcano's ash fall rotted some of the guava and peach fruits and had a drying effect on spearment and epazote plants. Even these small ash amounts could have hindered sugar cane and agave growth.

Group EDF annual report 2005 sustainable development; Groupe EDF rapport annuel 2005 developpement durable

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-05-15

The EDF Group's Sustainable Development Report for 2005 is designed to report on Group commitments particularly within its Agenda 21, its ethical charter, and the Global Compact. It has also been prepared with reference to external reference frameworks: the Global Reporting Initiative (GRI) guidelines and the French New Economic Regulations (NRE) contained in the May 15, 2001 French law. It contents the Chairman's statement, the evaluation of renewing and sharing commitments with all stakeholders, the managing local issues, EDF responses to the challenges of the future. Indicators are also provided. (A.L.B.)

Hymenochaete carpatica from Częstochowa Upland (S Poland

Directory of Open Access Journals (Sweden)

Lothar Krieglsteiner

2014-08-01

Full Text Available Hymenochaete carpatica has been found in 1994 in Czętochowa Upland, "Parkowre" nature reserve, in Wiercica river valley situated on calcareous area, 290-310 m above see level, on fissured bark of Acer pseudoplatanus. This contribution corresponds to the paper by Chlebicki (2003 who has recently discovered scveral localities of H. carpatica in Sudetes and Carpathians. Both papers present a set of sites of H. carpatica reported hitherto from Poland, and contribute to the knowledge on its distribution in Europe.

Online marketingová strategie portálu Divadlo.cz

OpenAIRE

Jermářová, Karolína

2010-01-01

The objective of this thesis is to propose an online marketing strategy for the Divadlo.cz web portal, which is currently undergoing a complete redesign including the extension of functions and services provided to its users. The proposed mix of marketing activities should help to increase the target groups' awareness of news on the web and provide the increase of the targeted visit rate. At the same time I focused on the possibilities of the increase of the web conversion ratio and, on the b...

Guidance on the environmental risk assessment of genetically modified plants

DEFF Research Database (Denmark)

Bartsch, Detlef; Chueca, Cristina; De-Schrijver, Adinda

risk evaluation. The scientific Panel on Genetically Modified Organisms (of the European Food Safety Authority (EFSA GMO Panel) considers seven specific areas of concern to be addressed by applicants and risk assessors during the ERA (1) persistence and invasiveness of the GM plant , or its compatible......This document provides guidance for the environmental risk assessment (ERA) of genetically modified (GM) plants submitted within the framework of Regulation (EC) No. 1829/2003 on GM food and feed or under Directive 2001/18/EC on the deliberate release into the environment of genetically modified...... organisms (GMOs). This document provides guidance for assessing potential effects of GM plants on the environment and the rationales for the data requirements for a comprehensive ERA of GM plants. The ERA should be carried out on a case-by-case basis, following a step-by-step assessment approach...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2015. Scientific Opinion on the substantiation of a health claim related to Symbiosal® and lowering of blood pressure and reduced risk of hypertension pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

2015-01-01

Following an application from Han-Asiabiotech GmbH, submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of Germany, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion...... in blood pressure when Symbiosal® was consumed instead of table salt for eight weeks in the context of a salt-restricted diet, but that no other human studies in which these results have been replicated were provided, that the animal studies did not support the results of the human study...

Calculation of dietary exposure to acrylamide in the Norwegian population

OpenAIRE

Norwegian Scientific Committee for Food Safety

2015-01-01

The Norwegian Scientific Committee for Food Safety (VKM) is requested by the Norwegian Food Safety Authority (NFSA) to calculate the dietary exposure to acrylamide in the Norwegian population. NFSA refers to the recent scientific opinion on acrylamide in food by the European Food Safety Authority (EFSA). EFSA concludes that acrylamide in food potentially increases the risk of developing cancer for consumers in all age groups.

Construction of a map-based reference genome sequence for barley, Hordeum vulgare L.

Czech Academy of Sciences Publication Activity Database

Beier, S.; Himmelbach, A.; Colmsee, C.; Zhang, X. Q.; Barrero, R. A.; Hastie, A.; Šimková, Hana; Staňková, Helena; Vrána, Jan; Chan, S.; Zhou, G.; Poland, J.; Bellgard, M. I.; Houben, A.; Doležel, Jaroslav; Ayling, S.; Lonardi, S.; Scholz, U.; Stein, N.; Mascher, M.

2017-01-01

Roč. 4, APR 27 (2017), č. článku 170044. ISSN 2052-4463 R&D Projects: GA MŠk(CZ) LO1204 Institutional support: RVO:61389030 Keywords : BACTERIAL ARTIFICIAL CHROMOSOMES * PHYSICAL MAP * LIBRARIES Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 4.836, year: 2016

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) ; Guidance on the scientific requirements for health claims related to physical performance

DEFF Research Database (Denmark)

Tetens, Inge

The Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked by the European Food Safety Authority (EFSA) to draft guidance on scientific requirements for health claims related to physical performance. This guidance has been drawn from scientific opinions of the NDA Panel on such health...... claims. Thus, this guidance document represents the views of the NDA Panel based on the experience gained to date with the evaluation of health claims in this area. It is not intended that the document should include an exhaustive list of beneficial effects and studies/outcome measures which...

The Electroluminescence Mechanism of Solution-Processed TADF Emitter 4CzIPN Doped OLEDs Investigated by Transient Measurements

Directory of Open Access Journals (Sweden)

Peng Wang

2016-10-01

Full Text Available High efficiency, solution-processed, organic light emitting devices (OLEDs, using a thermally-activated delayed fluorescent (TADF emitter, 1,2,3,5-tetrakis(carbazol-9-yl-4,6-dicyanobenzene (4CzIPN, are fabricated, and the transient electroluminescence (EL decay of the device with a structure of [ITO/PEDOT: PSS/4CzIPN 5 wt % doped 4,40-N,N0-dicarbazolylbiphenyl(CBP/bis-4,6-(3,5-di-4-pyridylphenyl-2-methylpyrimidine (B4PyMPM/lithium fluoride (LiF/Al], is systematically studied. The results shed light on the dominant operating mechanism in TADF-based OLEDs. Electroluminescence in the host–guest system is mainly produced from the 4CzIPN emitter, rather than the exciplex host materials.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sodium and maintenance of normal muscle function (ID 359) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to sodium and maintenance of normal muscle function. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders...... with a greater likelihood of increased blood pressure, which in turn has been directly related to the development of cardiovascular and renal diseases. For these reasons, national and international bodies have set targets for a reduction in dietary sodium intakes....

Groundwater level variations in the seismically active region of Western Bohemia in the years 2005-2010

Czech Academy of Sciences Publication Activity Database

Gaždová, Renata; Novotný, Oldřich; Málek, Jiří; Valenta, Jan; Brož, Milan; Kolínský, Petr

2011-01-01

Roč. 8, č. 1 (2011), s. 17-27 ISSN 1214-9705 R&D Projects: GA AV ČR IAA300460602 Institutional research plan: CEZ:AV0Z30460519 Keywords : Western Bohemia * earthquake swarm * groundwater level Subject RIV: DC - Siesmology, Volcanology, Earth Structure Impact factor: 0.530, year: 2011 http://www.irsm.cas.cz/abstracts/AGG/01_11/2_Gazdova.pdf

EFSA Panel on Food Contact Materials , Enzymes, Flavourings and Processing Aids (CEF); Scientific Opinion on Flavouring Group Evaluation 224 (FGE.224): Consideration of genotoxic potential for two α,β - unsaturated thiophenes from subgroup 5.2 of FGE.19 by EFSA

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Binderup, Mona-Lise; Lund, Pia

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of the European Food Safety Authority was requested to evaluate the genotoxic potential of two flavouring substances from subgroup 5.2 of FGE.19 in the Flavouring Group Evaluation 224 (FGE.224). The Flavour Industry has...... provided additional genotoxicity studies for one of the two substances in FGE.224, namely 5-methyl-2-thiophenecarbaldehyde [FL-no: 15.004]. The data requested by EFSA for the other substance, 3-acetyl-2,5-dimethylthiophene [FL-no: 15.024] of FGE.224 will be provided subsequently according to the Flavour...... are still pending and no conclusion could be drawn in the present FGE. © European Food Safety Authority, 2013...

Final Report on DANCEE-NFNA-NERI-NEPA-Gene Modified Organisms (GMO) Workshop for the Baltic Countries Estonia - Latvia - Lithuania in Collaboration with CEE Biosafety and BEF, January 23-30, 2001

DEFF Research Database (Denmark)

Løkke, H.; Damgaard, C.

Report on Training Cource on GMO Risk Assessment in Silkeborg. -- DANCEE - Environmental Assistance to Eastern Europe. (NEPA) is DEPA - (National) Danish Environmental Protection Agency (Miljøstyrelsen). NFNA - The National Forest and Nature Agency (Skov- og Naturstyrelsen). BEF - Baltic Environm...

PV Cz silicon manufacturing technology improvements

Science.gov (United States)

Jester, T.

1995-09-01

This describes work done in the final phase of a 3-y, 3-phase contract to demonstrate cost reductions and improvements in manufacturing technology. The work focused on near-term projects in the SSI (Siemens Solar Industries) Czochralski (Cz) manufacturing facility in Camarillo, CA; the final phase was concentrated in areas of crystal growth, wafer technology, and environmental, safety, and health issues. During this period: (1) The crystal-growing operation improved with increased growth capacity; (2) Wafer processing with wire saws continued to progress; the wire saws yielded almost 50 percent more wafers per inch in production. The wire saws needs less etching, too; (3) Cell processing improvements focused on better handling and higher mechanical yield. The cell electrical distribution improved with a smaller standard deviation in the distribution; and (4) Module designs for lower material and labor costs continued, with focus on a new junction box, larger modules with larger cells, and less costly framing techniques. Two modules demonstrating these cost reductions were delivered during this phase.

Novel GMO-Based Vaccines against Tuberculosis: State of the Art and Biosafety Considerations

Science.gov (United States)

Leunda, Amaya; Baldo, Aline; Goossens, Martine; Huygen, Kris; Herman, Philippe; Romano, Marta

2014-01-01

Novel efficient vaccines are needed to control tuberculosis (TB), a major cause of morbidity and mortality worldwide. Several TB vaccine candidates are currently in clinical and preclinical development. They fall into two categories, the one of candidates designed as a replacement of the Bacille Calmette Guérin (BCG) to be administered to infants and the one of sub-unit vaccines designed as booster vaccines. The latter are designed as vaccines that will be administered to individuals already vaccinated with BCG (or in the future with a BCG replacement vaccine). In this review we provide up to date information on novel tuberculosis (TB) vaccines in development focusing on the risk assessment of candidates composed of genetically modified organisms (GMO) which are currently evaluated in clinical trials. Indeed, these vaccines administered to volunteers raise biosafety concerns with respect to human health and the environment that need to be assessed and managed. PMID:26344627

Novel GMO-Based Vaccines against Tuberculosis: State of the Art and Biosafety Considerations

Directory of Open Access Journals (Sweden)

Amaya Leunda

2014-06-01

Full Text Available Novel efficient vaccines are needed to control tuberculosis (TB, a major cause of morbidity and mortality worldwide. Several TB vaccine candidates are currently in clinical and preclinical development. They fall into two categories, the one of candidates designed as a replacement of the Bacille Calmette Guérin (BCG to be administered to infants and the one of sub-unit vaccines designed as booster vaccines. The latter are designed as vaccines that will be administered to individuals already vaccinated with BCG (or in the future with a BCG replacement vaccine. In this review we provide up to date information on novel tuberculosis (TB vaccines in development focusing on the risk assessment of candidates composed of genetically modified organisms (GMO which are currently evaluated in clinical trials. Indeed, these vaccines administered to volunteers raise biosafety concerns with respect to human health and the environment that need to be assessed and managed.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to acetyl-L-carnitine and contribution to normal cognitive function (ID 1432) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to acetyl-L-carnitine and contribution to normal cognitive function. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... be drawn for the scientific substantiation of the claim. On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the consumption of acetyl-L-carnitine and contribution to normal cognitive function....

Návrh strategie inbound marketingu pro start-up Stips.cz

OpenAIRE

Horáčková, Simona

2017-01-01

The aim of this diploma thesis is to analyze and create an inbound marketing strategy for e-shop Stips.cz, which sells experience gifts. The theoretical part includes describing what inbound marketing is and the phases and rules of inbound marketing. In another part many tools of inbound marketing are covered. In the practical part a detailed analysis of visits to the website, competitors, SEO factors and inbound marketing tools is conducted. After the analysis the inbound marketing strategy ...

Current developments in CZ Si crystal growing technology; Aktuelle Entwicklungen in der Zuechtungstechnologie von CZ-Si-Kristallen

Energy Technology Data Exchange (ETDEWEB)

Altekrueger, Burkhard; Volk, Michael [PVA TePla AG, Wettenberg (Germany)

2011-02-15

The industrial growing of increasingly large and perfect silicon (Si) monocrystals for applications in microelectronics and photovoltaics requires continuous improvement of process control and growing technology. Continuous adaptation and optimization of system technology in terms of reliability, process flexibility and dimensioning are also necessary. The basic principles of industrial silicon crystal growing and the resultant requirements for the Si process and system technologies are described in the first part of this series of articles. The constantly increasing requirements for the performance and complexity of the electronic circuits (chips) in accordance with Moore's Law mean that the requirements for the perfection and dimensions of monocrystalline Si wafers and Si crystals are also continuously rising. After the introduction of the 300 mm Si wafer generation in recent years, the next Si wafer generation (450 mm) is therefore being discussed already. The technological and economic effects of these constantly increasing requirements for the necessary system technologies will be set out and discussed in the subsequent articles on the basis of current Si CZ crystal growing systems as well as new system concepts. (orig.)

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2013. Scientific Opinion on the substantiation of a health claim related to Preservation ® and “ rapid recovery of cellular activity post stress ” pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from ICP Ltd, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Malta, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...... substantiation of a health claim related to Preservation® and “rapid recovery of cellular activity post stress”. The Panel considers that Preservation®, which contains an extract of prickly pear cactus Opuntia ficus-indica, is sufficiently characterised. The claimed effect is “rapid recovery of cellular activity...

EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2016. Scientific opinion on Flavouring Group Evaluation 400 (FGE.400): 3-(1- ((3,5-dimethylisoxazol-4-yl)methyl)-1H-pyrazol-4-yl)-1-(3-hydroxybenzyl)imidazolidine-2,4-dione

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Nørby, Karin Kristiane

modifier in specific categories of food. There is no safety concern with respect to genotoxicity. A 90-day dietary administration study in rats showed no adverse effects for doses up to 100 mg/kg body weight (bw) per day, providing an adequate margin of safety. Developmental toxicity was not observed...... for various foods in different food categories.......The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (CEF) of EFSA was requested to deliver a scientific opinion on the implications for human health of the flavouring substance 3-(1-((3,5-dimethylisoxazol-4-yl)methyl)-1H-pyrazol-4-yl)-1-(3-hydroxybenzyl)imidazolidine-2...

Using scientific evidence to inform public policy on the long distance transportation of animals: role of the European Food Safety Authority

Directory of Open Access Journals (Sweden)

Oriol Ribò

2008-03-01

Full Text Available The authors review the work of the previous Scientific Committee on Animal Health and Animal Welfare and the current European Food Safety Authority (EFSA in providing scientific advice on the welfare aspects of animal transport and the impact of this advice on the European Union (EU regulatory framework. Through its Protocol on the Protection and Welfare of Animals, the Treaty of Amsterdam obliges European institutions to pay full regard to the welfare requirements of animals when formulating and implementing EU legislation. Regulation 1/2005 states that EU legislation should be amended to take into account new scientific evidence. Provisions for poultry, cats and dogs take into account the recommendations included in EFSA's Scientific Opinion which considers different species (poultry, deer, rabbits, dogs and cats, fish and exotic animals. Examples of the effect of the scientifically based conclusions and recommendations from the Scientific Opinion on the measures in Regulation 1/2005 are summarised and show the impact of scientific evidence on EU legislation.

Using scientific evidence to inform public policy on the long distance transportation of animals: role of the European Food Safety Authority.

Science.gov (United States)

Ribò, Oriol; Candiani, Denise; Aiassa, Elisa; Correia, Sandra; Afonso, Ana; De Massis, Fabrizio; Serratosa, Jordi

2008-01-01

The authors review the work of the previous Scientific Committee on Animal Health and Animal Welfare and the current European Food Safety Authority (EFSA) in providing scientific advice on the welfare aspects of animal transport and the impact of this advice on the European Union (EU) regulatory framework. Through its Protocol on the Protection and Welfare of Animals, the Treaty of Amsterdam obliges European institutions to pay full regard to the welfare requirements of animals when formulating and implementing EU legislation. Regulation 1/2005 states that EU legislation should be amended to take into account new scientific evidence. Provisions for poultry, cats and dogs take into account the recommendations included in EFSA's Scientific Opinion which considers different species (poultry, deer, rabbits, dogs and cats, fish and exotic animals). Examples of the effect of the scientifically based conclusions and recommendations from the Scientific Opinion on the measures in Regulation 1/2005 are summarised and show the impact of scientific evidence on EU legislation.

Scientific Opinion on the substantiation of health claims related to various food(s)/food constituent(s) and “energy and vitality” (ID 18, 26, 62, 105, 122, 145, 165, 3962, 4054, 4440), “invigoration of the body” (ID 2383, 2386, 2391, 2393, 2409, 2441, 2463, 2488, 3834, 3883), “general health” (ID, 1313, 3348, 4182, 4613), “rejuvenation” (ID 3981, 4023), “tonic” (ID 1703, 3462, 3581, 4418), “stimulant” (ID 3190, 3506) and “metabolic benefits” (ID 4438) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

EFSA-Q-2008-3196,EFSA-Q-2008-3221,EFSA-Q-2008-3922,EFSA-Q-2008-4079,EFSA-Q-2008-4191,EFSA-Q-2008-4233,EFSA-Q-2008-4307,EFSA-Q-2008-4550,EFSA-Q-2008-4599,EFSA-Q-2008-4678,EFSA-Q-2008-4696,EFSA-Q-2008-4735,EFSA-Q-2008-4766,EFSA-Q-2008-4893,EFSA-Q-2010-00371 , EFSA-Q-2008-805,EFSA-Q-2008-813,EFSA-Q-...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to fructo-oligosaccharides (FOS) and decreasing potentially pathogenic gastro-intestinal microorganisms (ID 781), pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to fructo-oligosaccharides and decreasing potentially pathogenic gastro-intestinal microorganisms. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... conclusions could be drawn for the scientific substantiation of the claim were provided. On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the consumption of fructo-oligosaccharides and decreasing potentially pathogenic gastro...

Scientific Opinion on the substantiation of health claims related to various food(s)/food constituent(s) claiming biotransformation of xenobiotic substances (ID 1378, 2388, 2401, 3900, 3942, 4039, 4510, 4513, 4544, 4628, 4639), “elimination”, “cleansing” and “purification” (ID 1347, 4024, 4442

DEFF Research Database (Denmark)

Tetens, Inge

EFSA-Q-2008-2084 , EFSA-Q-2008-2115 , EFSA-Q-2008-2620 , EFSA-Q-2008-3121 , EFSA-Q-2008-3134 , EFSA-Q-2008-3888 , EFSA-Q-2008-4616 , EFSA-Q-2008-4658 , EFSA-Q-2008-4736 , EFSA-Q-2008-4751 , EFSA-Q-2010-00395 , EFSA-Q-2010-00410 , EFSA-Q-2010-00463 , EFSA-Q-2010-00466 , EFSA-Q-2010-00497 , EFSA-Q-...

Scientific Opinion on the substantiation of health claims related to eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), docosapentaenoic acid (DPA) and maintenance of normal cardiac function (ID 504, 506, 516, 527, 538, 703, 1128, 1317, 1324, 1325), maintenance of normal blood glucose, -cholesterol concentrations (ID 527, 538, 1317, 1325, 4689), protection of the skin from photo-oxidative (UV-induced) damage (ID 530), improved absorption of EPA and DHA (ID 522, 523)

DEFF Research Database (Denmark)

Tetens, Inge

EFSA-Q-2008-1291 , EFSA-Q-2008-1293 , EFSA-Q-2008-1303 , EFSA-Q-2008-1307 , EFSA-Q-2008-1309 , EFSA-Q-2008-1310 , EFSA-Q-2008-1314 , EFSA-Q-2008-1317 , EFSA-Q-2008-1325 , EFSA-Q-2008-1353 , EFSA-Q-2008-1490 , EFSA-Q-2008-1867 , EFSA-Q-2008-2054 , EFSA-Q-2008-2061 , EFSA-Q-2008-2062 , EFSA-Q-2008-...

Scientific Opinion on the substantiation of health claims related to non characterised bacteria and yeasts pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

EFSA-Q-2008-3668 , EFSA-Q-2008-3669 , EFSA-Q-2008-3670 , EFSA-Q-2008-3671 , EFSA-Q-2008-3672 , EFSA-Q-2008-3673 , EFSA-Q-2008-3674 , EFSA-Q-2008-3675 , EFSA-Q-2008-3676 , EFSA-Q-2008-3677 , EFSA-Q-2008-3692 , EFSA-Q-2008-3693 , EFSA-Q-2008-3697 , EFSA-Q-2008-3700 , EFSA-Q-2008-3701 , EFSA-Q-2008-...

Computer Science Research Institute 2005 annual report of activities.

Energy Technology Data Exchange (ETDEWEB)

Watts, Bernadette M.; Collis, Samuel Scott; Ceballos, Deanna Rose; Womble, David Eugene

2008-04-01

This report summarizes the activities of the Computer Science Research Institute (CSRI) at Sandia National Laboratories during the period January 1, 2005 to December 31, 2005. During this period, the CSRI hosted 182 visitors representing 83 universities, companies and laboratories. Of these, 60 were summer students or faculty. The CSRI partially sponsored 2 workshops and also organized and was the primary host for 3 workshops. These 3 CSRI sponsored workshops had 105 participants, 78 from universities, companies and laboratories, and 27 from Sandia. Finally, the CSRI sponsored 12 long-term collaborative research projects and 3 Sabbaticals.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the safety of astaxanthin-rich ingredients (AstaREAL A1010 and AstaREAL L10) as novel food ingredients

DEFF Research Database (Denmark)

Tetens, Inge; Poulsen, Morten

2014-01-01

/day. Mean and high-level (95th percentile) daily intakes of 0.106 mg/kg bw and 0.256 mg/kg bw astaxanthin from the NFIs were estimated, based on European consumption data of the proposed food categories. The consumption of the NFIs is not considered to be nutritionally disadvantageous. There are no safety....... The Panel notes that the maximum recommended intake of 4 mg astaxanthin per day (0.06 mg/kg bw) and the estimated mean intake based on the use levels in the proposed food categories (0.106 mg/kg bw per day) exceed the ADI by approximately two- and three-fold, respectively. The Panel therefore concludes......Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on the safety of astaxanthin-rich ingredients AstaREAL A1010 and AstaREAL L10 as novel food ingredients (NFIs) in the context of Regulation...

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the extension of use for DHA and EPA-rich algal oil from Schizochytrium sp. as a Novel Food ingredient

DEFF Research Database (Denmark)

Tetens, Inge; Poulsen, Morten

2014-01-01

Following a request from the European Commission, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver a scientific opinion on an extension of use for docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA)-rich algal oil from Schizochytrium sp. as a novel food...... population, excluding pregnant and lactating women. In a previous opinion on the Tolerable Upper Intake Level of EPA, DHA and docosapentaenoic acid (DPA), the Panel concluded that supplemental intake of EPA and DHA combined at doses up to 5 g/day, does not give rise to safety concerns for adults. Based...... ingredient (NFI) in the context of Regulation (EC) No 258/97. The NFI is already authorised for use in a range of foodstuffs at specified maximum levels. The applicant requests an extension of use of the NFI in food supplements up to a maximum DHA and EPA content of 3 g per daily dose for the adult...

Effect of endogenous reference genes on digital PCR assessment of genetically engineered canola events

Directory of Open Access Journals (Sweden)

Tigst Demeke

2018-05-01

Full Text Available Droplet digital PCR (ddPCR has been used for absolute quantification of genetically engineered (GE events. Absolute quantification of GE events by duplex ddPCR requires the use of appropriate primers and probes for target and reference gene sequences in order to accurately determine the amount of GE materials. Single copy reference genes are generally preferred for absolute quantification of GE events by ddPCR. Study has not been conducted on a comparison of reference genes for absolute quantification of GE canola events by ddPCR. The suitability of four endogenous reference sequences (HMG-I/Y, FatA(A, CruA and Ccf for absolute quantification of GE canola events by ddPCR was investigated. The effect of DNA extraction methods and DNA quality on the assessment of reference gene copy numbers was also investigated. ddPCR results were affected by the use of single vs. two copy reference genes. The single copy, FatA(A, reference gene was found to be stable and suitable for absolute quantification of GE canola events by ddPCR. For the copy numbers measured, the HMG-I/Y reference gene was less consistent than FatA(A reference gene. The expected ddPCR values were underestimated when CruA and Ccf (two copy endogenous Cruciferin sequences were used because of high number of copies. It is important to make an adjustment if two copy reference genes are used for ddPCR in order to obtain accurate results. On the other hand, real-time quantitative PCR results were not affected by the use of single vs. two copy reference genes. Keywords: Canola, Digital PCR, DNA extraction, GMO, Reference genes

Bioadhesive floating microsponges of cinnarizine as novel gastroretentive delivery: Capmul GMO bioadhesive coating versus acconon MC 8-2 EP/NF with intrinsic bioadhesive property.

Science.gov (United States)

Raghuvanshi, Smita; Pathak, Kamla

2016-01-01

The study was aimed at the development of low-density gastroretentive bioadhesive microsponges of cinnarizine by two-pronged approach (i) coating with bioadhesive material and (ii) exploration of acconon MC 8-2 EP/NF as bioadhesive raw material for fabrication. Microsponges were prepared by quasi-emulsion solvent diffusion method using 3 2 factorial design. Capmul GMO was employed for bioadhesive coating. In parallel, potential of acconon for the fabrication of bioadhesive floating microsponges (A8) was assessed. Formulation with entrapment efficiency = 82.4 ± 3.4%, buoyancy = 82.3 ± 2.5%, and correlation of drug release (CDR 8h ) = 88.7% ± 2.9% was selected as optimized formulation (F8) and subjected to bioadhesive coating (BF8). The %CDR 8h for A8 was similar to BF8 (87.2% ± 3.5%). Dynamic in vitro bioadhesion test revealed comparable bioadhesivity with BF8. The ex vivo permeation across gastric mucin displayed 63.16% for BF8 against 56.74% from A8; affirmed the bioadhesivity of both approaches. The study concluded with the development of novel bioadhesive floating microsponges of cinnarizine employing capmul GMO as bioadhesive coating material and confirmed the viability of acconon MC 8-2EP/NF as bioadhesive raw material for sustained targeted delivery of drug.

Does cation ordering in omphacite influence development of lattice-referred orientation?

Czech Academy of Sciences Publication Activity Database

Ulrich, Stanislav; Mainprice, D.

2005-01-01

Roč. 27, č. 3 (2005), s. 419-431 ISSN 0191-8141 R&D Projects: GA ČR GP205/03/P065 Institutional research plan: CEZ:AV0Z30120515 Keywords : clinopyroxene * lattice-preferred orientation * cation ordering Subject RIV: DB - Geology ; Mineralogy Impact factor: 2.109, year: 2005

Scientific Opinion on the substantiation of health claims related to various food(s)/food constituent(s) and improved bioavailability of nutrients (ID 384, 1728, 1752, 1755), energy and nutrient supply (ID 403, 413, 457, 487, 667, 1675, 1710, 2901, 4496) and presence of a nutrient in the human body

DEFF Research Database (Denmark)

Tetens, Inge

EFSA-Q-2008-1171 , EFSA-Q-2008-1190 , EFSA-Q-2008-1200 , EFSA-Q-2008-1244 , EFSA-Q-2008-1274 , EFSA-Q-2008-1454 , EFSA-Q-2008-1507 , EFSA-Q-2008-2411 , EFSA-Q-2008-2446 , EFSA-Q-2008-2464 , EFSA-Q-2008-2485 , EFSA-Q-2008-2488 , EFSA-Q-2008-3634 , EFSA-Q-2010-00449...

On the intrinsic gettering in Cu-contaminated Cz-Si

International Nuclear Information System (INIS)

Schmalz, K.; Kirscht, F.G.; Babanskaja, I.; Kittler, M.; Richter, H.; Seifert, W.

1985-01-01

NAA, TEM, DLTS, and EBIC investigations of Cu-contaminated Cz-Si with an intrinsic-gettering procedure realized by a multistep heat treatment demonstrate the efficiency of intrinsic gettering concerning redistribution to volume defects for Cu-contamination up to 10 16 cm -3 . The Cu-precipitation at the surface can still be suppressed for such contamination due to volume defects. However, in this case the concentration of contamination induced deep level centers at the surface is independent of the volume defect density. Only for Cu-contamination of about 10 15 cm -3 the gettering effect is strong enough to prevent the introduction of contamination induced deep levels at the surface. (author)

EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids), 2016. Scientific opinion on Flavouring Group Evaluation 313, (FGE.313): α,β-unsaturated 3(2H)-furanone derivatives from chemical group 13

DEFF Research Database (Denmark)

Beltoft, Vibe Meister; Nørby, Karin Kristiane

The Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids of EFSA was requested to evaluate three flavouring substances, 2,5-dimethyl-4-ethoxyfuran-3(2H)-one [FL-no: 13.117], 2,5-dimethylfuran-3(2H)-one [FL-no: 13.119] and 4-Acetyl-2,5-dimethylfuran-3(2H)-one [FL-no: 13......–activity relationships, intake from current uses, toxicological threshold of concern, and available data on metabolism and toxicity. The Panel concluded that the two flavouring substances [FL-no: 13.117, 13.119] do not give rise to safety concerns at their level of dietary intake, estimated on the basis of the Maximised...... Survey-derived Daily Intake (MSDI) approach. For the flavouring substance [FL-no: 13.175], toxicity data are required. Besides the safety assessment of the flavouring substance, the specifications for the materials of commerce have also been considered. Adequate specifications including complete purity...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to ribose and faster recovery from muscle fatigue after exercise (ID 4226) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to ribose and faster recovery from muscle fatigue after exercise. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from...... stakeholders. The food that is the subject of the health claim is ribose. The Panel considers that ribose is sufficiently characterised. The claimed effect is “maintenance of ATP levels, exercise performance, exercise recovery”. The target population is assumed to be adults performing strenuous exercise...

Scientific Opinion on Review of the European Union Summary Report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks—Terms of reference 2 to 7

OpenAIRE

EFSA Panel on Animal Health and Welfare (AHAW)

2013-01-01

The Animal Health and Welfare (AHAW) Panel of the European Food Safety Authority (EFSA) has evaluated the European Union Summary Report on Trends and Sources of Zoonoses, Zoonotic Agents and Food-borne Outbreaks by EFSA and ECDC (the report) with regard to data needs and subsequent analyses that will minimise the impact of existing data gaps and inconsistencies. Specific assessments performed for bovine tuberculosis, echinococcosis, Q fever, brucellosis, rabies, cysticercosis and tularaemia s...

Prevalence of Listeria monocytogenes in European cheeses

DEFF Research Database (Denmark)

Martinez Rios, Veronica; Dalgaard, Paw

2017-01-01

, respectively, pasteurized or un-pasteurized milk. Data from a total of 130,604 samples were analysed. Mean prevalence for presence during 2005-2015 estimated from scientific literature (2.3% with confidence interval (CI): 1.4-3.8%) was more than three times higher than results from EFSA reports (0.7%; CI: 0.......05) for cheeses produced from pasteurized (0.9%; CI: 0.4-1.9%) or un-pasteurized (1.0%; CI: 0.4-2.2%) milk. For cheese samples reported by EFSA 0.2% CI: 0.1-0.4% had concentration of L. monocytogenes above the critical European limits of 100 cfu/g. In addition, this systematic review focused on groups...

Radioactive waste management profiles. A compilation of data from the Net Enabled Waste Management Database (NEWMDB). No. 7, December 2005 (last updated 2005.12.15)

International Nuclear Information System (INIS)

2005-12-01

This Profiles report is based on data collected using the NEWMDB from May to October 2005. The report was first published on line within the NEWMDB December 2005. Starting with NEWMDB version II (introduced January 2004) individual reports can be updated after a Profiles report is published. Please refer to the Profiles bookmark; the page that is accessed via this bookmark lists revisions to individual Profiles (if there are any)

Food selection by bacterivorous protists: insight from the analysis of the food vacuole content by means of fluorescence in situ hybridization

Czech Academy of Sciences Publication Activity Database

Jezbera, Jan; Horňák, Karel; Šimek, Karel

2005-01-01

Roč. 52, č. 3 (2005), s. 351-363 ISSN 0168-6496. [Symposium on Aquatic Microbial Ecology /9./. Helsinky, 21.08.2005-26.08.2005] R&D Projects: GA ČR(CZ) GA206/02/0003; GA ČR(CZ) GA206/05/0007 Grant - others:MŠMT(CZ) 1058/2004 Institutional research plan: CEZ:AV0Z60170517 Keywords : hybridization * grazing * flagellates Subject RIV: DA - Hydrology ; Limnology Impact factor: 2.787, year: 2005

Nutrition issues in Codex: health claims, nutrient reference values and WTO agreements: a conference report.

Science.gov (United States)

Aggett, Peter J; Hathcock, John; Jukes, David; Richardson, David P; Calder, Philip C; Bischoff-Ferrari, Heike; Nicklas, Theresa; Mühlebach, Stefan; Kwon, Oran; Lewis, Janine; Lugard, Maurits J F; Prock, Peter

2012-03-01

Codex documents may be used as educational and consensus materials for member governments. Also, the WTO SPS Agreement recognizes Codex as the presumptive international authority on food issues. Nutrient bioavailability is a critical factor in determining the ability of nutrients to provide beneficial effects. Bioavailability also influences the quantitative dietary requirements that are the basis of nutrient intake recommendations and NRVs. Codex, EFSA and some national regulatory authorities have established guidelines or regulations that will permit several types of health claims. The scientific basis for claims has been established by the US FDA and EFSA, but not yet by Codex. Evidence-based nutrition differs from evidence-based medicine, but the differences are only recently gaining recognition. Health claims on foods may provide useful information to consumers, but many will interpret the information to mean that they can rely upon the food or nutrient to eliminate a disease risk. NRVs are designed to provide a quantitative basis for comparing the nutritive values of foods, helping to illustrate how specific foods fit into the overall diet. The INL-98 and the mean of adult male and female values provide NRVs that are sufficient when used as targets for individual intakes by most adults. WTO recognizes Codex as the primary international authority on food issues. Current regulatory schemes based on recommended dietary allowances are trade restrictive. A substantial number of decisions by the EFSA could lead to violation of WTO agreements.

Group EDF annual report 2005 sustainable development

International Nuclear Information System (INIS)

2006-05-01

The EDF Group's Sustainable Development Report for 2005 is designed to report on Group commitments particularly within its Agenda 21, its ethical charter, and the Global Compact. It has also been prepared with reference to external reference frameworks: the Global Reporting Initiative (GRI) guidelines and the French New Economic Regulations (NRE) contained in the May 15, 2001 French law. It contents the Chairman's statement, the evaluation of renewing and sharing commitments with all stakeholders, the managing local issues, EDF responses to the challenges of the future. Indicators are also provided. (A.L.B.)

Muscarinic antagonism impairs navigation in the active allothetic place avoidance task

Czech Academy of Sciences Publication Activity Database

Stuchlík, Aleš; Valeš, Karel

2005-01-01

Roč. 6, č. Suppl.1 (2005), s. 376-376 ISSN 1562-2975. [WFSBP Congress /8./. 28.06.2005-03.07.2005, Vienna] R&D Projects: GA MŠk(CZ) 1M0517; GA MŠk(CZ) LC554; GA ČR(CZ) GP309/03/P126 Institutional research plan: CEZ:AV0Z50110509 Keywords : AAPA Subject RIV: FH - Neurology

Effect of clozapine on Active Allothetic Place Avoidance in an animal model of schizophrenia

Czech Academy of Sciences Publication Activity Database

Valeš, Karel; Bubeníková, Věra; Stuchlík, Aleš

2005-01-01

Roč. 6, č. Suppl.1 (2005), s. 296-296 ISSN 1562-2975. [WFSBP Congress /8./. 28.06.2005-03.07.2005, Vienna] R&D Projects: GA ČR(CZ) GP309/03/P126; GA MŠk(CZ) 1M0517; GA MŠk(CZ) LC554 Institutional research plan: CEZ:AV0Z50110509 Keywords : AAPA Subject RIV: FH - Neurology

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on Dietary Reference Values for energy

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies (NDA) derived dietary reference values for energy, which are provided as average requirements (ARs) of specified age and sex groups. For children and adults, total energy expenditure (TEE...

Extracellular phosphatase activity of freshwater phytoplankton exposed to different .i.in situ./i. phosphorus concentrations

Czech Academy of Sciences Publication Activity Database

Štrojsová, Alena; Vrba, Jaroslav; Nedoma, Jiří; Šimek, Karel

2005-01-01

Roč. 56, č. 4 (2005), s. 417-424 ISSN 1323-1650. [Symposium for European Freshwater Sciences /4./. Krakow, 22.08.2005-26.08.2005] R&D Projects: GA ČR(CZ) GA206/02/0003; GA AV ČR(CZ) IAA6017202 Grant - others:FRVŠ(CZ) G4 1841 Institutional research plan: CEZ:AV0Z60170517 Keywords : ELF97 phosphate * image cytometry * species-specific activity Subject RIV: EH - Ecology, Behaviour Impact factor: 1.478, year: 2005

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sodium alginate and reduction of post-prandial glycaemic responses (ID 1868, 1881) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to sodium alginate and reduction of post-prandial glycaemic responses. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from......Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... from which conclusions could be drawn for the scientific substantiation of the claim did not show a reduction in post-prandial glycaemic responses without a disproportionate increase in post-prandial insulinaemic responses following consumption of sodium alginate. On the basis of the data presented...

Results of a desk study on best practices for animal transport

NARCIS (Netherlands)

Mitchell, M.A.; Spoolder, H.A.M.

2016-01-01

The EU has provides a harmonised legal framework for animal transport. Part of that framework is the EU adopted Regulation (EC) 1/2005. The content and impact of the Regulation has been the subject of a Scientific Opinion by EFSA in 2011 followed in 2012 by an impact report from the Commission to

Ženy a (re)distribuce moci

Czech Academy of Sciences Publication Activity Database

Maříková, Hana

2005-01-01

Roč. 6, č. 1 (2005), s. 46-51 ISSN 1213-0028. [Mass Media in (Re)Distribution of Power. Riga, 12.01.2005-14.01.2005] R&D Projects: GA AV ČR(CZ) 1QS700280503; GA AV ČR(CZ) IAA700280504 Institutional research plan: CEZ:AV0Z70280505 Keywords : gender equality * power * massmedia Subject RIV: AO - Sociology, Demography www.genderonline.cz

Jaekaubandus kaotab kaubakadudest 1,27% käibest

Index Scriptorium Estoniae

2006-01-01

Ilmunud ka: Finansovõi Menedzhment : infovõpusk nr. 3 apr. lk. 8. Briti firma The Centre for Retail Research uuring näitas, et Baltimaade suurte jaekaubandusfirmade keskmine kaubakadu 2005. aastal oli 1,27% käibest. Tabel: Euroopa jaekaubafirmade kahjud kadude tõttu (% käibest)

Monitoring the presence of genetically modified food on the market of the Republic of Croatia.

Science.gov (United States)

Cattunar, Albert; Capak, Krunoslav; Novak, Jelena Zafran; Mićović, Vladimir; Doko-Jelinić, Jagoda; Malatestinić, Dulija

2011-12-01

From the beginning of the human race people have been applying different methods to change the genetic material of either plants or animals in order to increase their yield as well as to improve the quality and quantity of food. Genetically modified organism (GMO) means an organism in which the genetic material has been altered in a way that does not occur naturally by mating and/or natural recombination. Analysing the presence of GMO in food is done by detecting the presence of either specific DNA sequences inserted in the genome of transgenic organism, or detecting proteins as a result of the expression of the inserted DNA. In this work food testing for the presence of genetically modified organisms was conducted during the period from 2004 to 2007 in the GMO laboratory of the Croatian National Institute of Public Health. According to the regulations, among the samples in which the presence of GMO was detected, all those which had more than 0.9% of GMO content were either rejected from the border or removed from the market, because such GM food has to be appropriately labelled. Among the food samples which were analysed in 2004: 127 (2.37%) of a total of 1226 samples contained more than 0.9% of GMOs; in 2005 there was only one in 512 (0.20%) samples in total; in 2006 there were 4 out of 404 samples (0.99%), and in 2007: 7 of a total of 655 samples (1.07%) had GMO content above the allowed threshold of 0.9%.

Immune escape phenotype of HPV16-associated tumours: MHC class I expression changes during progression and therapy

Czech Academy of Sciences Publication Activity Database

Mikyšková, Romana; Bubeník, Jan; Vonka, V.; Šmahel, M.; Indrová, Marie; Bieblová, Jana; Šímová, Jana; Jandlová, Táňa

2005-01-01

Roč. 26, č. 2 (2005), s. 521-527 ISSN 1019-6439 R&D Projects: GA MZd(CZ) NC7148; GA MZd(CZ) NR8004; GA MZd(CZ) NR7807; GA ČR(CZ) GA301/04/0492; GA AV ČR(CZ) IAA5052203 Institutional research plan: CEZ:AV0Z5052915 Keywords : HPV16 * MHC class I- tumour cells * CTLs Subject RIV: EC - Immunology Impact factor: 2.681, year: 2005

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to beta-palmitate and contribution to softening of stools pursuant to Article 14 of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

2014-01-01

Following an application from Specialised Nutrition Europe (formerly IDACE), submitted for authorisation of a health claim pursuant to Article 14 of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked...... to deliver an opinion on the scientific substantiation of a health claim related to beta-palmitate and contribution to softening of stools. The food constituent, beta-palmitate, that is the subject of the health claim, is sufficiently characterised. Contribution to softening of stools is a beneficial...... physiological effect for infants. In weighing the evidence the Panel took into account that, out of two human intervention studies with important methodological limitations, one suggested a stool-softening effect of beta-palmitate whereas the second did not, that one animal study did not support a stool...

Regulation of Cys-based protein tyrosine phosphatases via reactive oxygen and nitrogen species in mast cells and basophils

Czech Academy of Sciences Publication Activity Database

Heneberg, Petr; Dráber, Petr

2005-01-01

Roč. 12, č. 16 (2005), s. 1859-1871 ISSN 0929-8673 R&D Projects: GA ČR(CZ) GA204/03/0594; GA ČR(CZ) GA301/03/0596; GA AV ČR(CZ) IAA5052310; GA MZd(CZ) NR8079; GA MŠk(CZ) 1M0506; GA MŠk(CZ) 1P04OE158 Institutional research plan: CEZ:AV0Z50520514 Keywords : mast cell * tyrosine phosphatase * reactive oxygen species Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 4.904, year: 2005

Combined astrometric catalogue EOC-3 An improved reference frame for long-term Earth rotation studies

Czech Academy of Sciences Publication Activity Database

Vondrák, Jan; Štefka, Vojtěch

2007-01-01

Roč. 463, č. 2 (2007), s. 783-788 ISSN 0004-6361 R&D Projects: GA MŠk(CZ) LC506 Institutional research plan: CEZ:AV0Z10030501 Keywords : reference systems * astrometry * catalogs Subject RIV: BN - Astronomy, Celestial Mechanics, Astrophysics Impact factor: 4.259, year: 2007

Distortion of genetically modified organism quantification in processed foods: influence of particle size compositions and heat-induced DNA degradation.

Science.gov (United States)

Moreano, Francisco; Busch, Ulrich; Engel, Karl-Heinz

2005-12-28

Milling fractions from conventional and transgenic corn were prepared at laboratory scale and used to study the influence of sample composition and heat-induced DNA degradation on the relative quantification of genetically modified organisms (GMO) in food products. Particle size distributions of the obtained fractions (coarse grits, regular grits, meal, and flour) were characterized using a laser diffraction system. The application of two DNA isolation protocols revealed a strong correlation between the degree of comminution of the milling fractions and the DNA yield in the extracts. Mixtures of milling fractions from conventional and transgenic material (1%) were prepared and analyzed via real-time polymerase chain reaction. Accurate quantification of the adjusted GMO content was only possible in mixtures containing conventional and transgenic material in the form of analogous milling fractions, whereas mixtures of fractions exhibiting different particle size distributions delivered significantly over- and underestimated GMO contents depending on their compositions. The process of heat-induced nucleic acid degradation was followed by applying two established quantitative assays showing differences between the lengths of the recombinant and reference target sequences (A, deltal(A) = -25 bp; B, deltal(B) = +16 bp; values related to the amplicon length of the reference gene). Data obtained by the application of method A resulted in underestimated recoveries of GMO contents in the samples of heat-treated products, reflecting the favored degradation of the longer target sequence used for the detection of the transgene. In contrast, data yielded by the application of method B resulted in increasingly overestimated recoveries of GMO contents. The results show how commonly used food technological processes may lead to distortions in the results of quantitative GMO analyses.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to oligomeric procyanidins (OPCs) from grape (Vitis vinifera L.) seeds and improvement of visual adaptation to the dark (ID 680) pursuant to Article 13(1) of Regulation

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to oligomeric procyanidins (OPCs) from grape (Vitis vinifera L.) seeds and improvement of visual adaptation to the dark. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA...... has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claim is oligomeric procyanidins (OPCs) from grape (Vitis vinifera L.) seeds. The Panel considers that OPCs from grape (Vitis vinifera L.) seeds are sufficiently characterised....... The claimed effect is “ocular system: OPCs play an important role in the structure and physiological functions of cornea, OPCs protect the cornea and improve visual adaptation to low luminances”. The target population is assumed to be the general population. In the context of the proposed wordings...

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to caffeine and theobromine in cocoa (Theobroma cacao L.) and enhancement of mood (ID 4276) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... claims in relation to caffeine and theobromine in cocoa (Theobroma cacao L.) and enhancement of mood. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States...... or directly from stakeholders. The food that is the subject of the health claim is cocoa (Theobroma cacao L.). The Panel considers that whereas the food, cocoa (Theobroma cacao L.), is not sufficiently characterised in relation to the claimed effect evaluated in this opinion, the food constituents, caffeine...

The International Reference Ionosphere 2012 – a model of international collaboration

Czech Academy of Sciences Publication Activity Database

Bilitza, D.; Altadill, D.; Zhang, Y.; Mertens, Ch.; Truhlík, Vladimír; Richards, P.; McKinnell, L.- A.; Reinisch, B.

2014-01-01

Roč. 4, 20 February (2014), A07/1-A07/12 ISSN 2115-7251 R&D Projects: GA MŠk(CZ) LH11123 Institutional support: RVO:68378289 Keywords : International Reference Ionosphere * empirical models * plasma parameters * real - time IRI Subject RIV: DG - Athmosphere Sciences, Meteorology Impact factor: 2.558, year: 2014 http://www.swsc-journal.org/articles/swsc/abs/2014/01/swsc130043/swsc130043.html

Microstructure stability and creep behaviour of advanced high chromium ferritic steels

Czech Academy of Sciences Publication Activity Database

Sklenička, Václav; Kuchařová, Květa; Kudrman, J.; Svoboda, Milan; Kloc, Luboš

43 2005, č. 1 (2005), s. 20-33 ISSN 0023-432X R&D Projects: GA ČR(CZ) GA106/02/0608; GA AV ČR(CZ) IAA2041101; GA AV ČR(CZ) 1QS200410502 Institutional research plan: CEZ:AV0Z20410507 Keywords : 9-12%Cr steels * microstructure stability * creep behaviour * nonsteady creep loading Subject RIV: JG - Metallurgy Impact factor: 0.973, year: 2005

Auditory evoked potential P300 in adults: reference values.

Science.gov (United States)

Didoné, Dayane Domeneghini; Garcia, Michele Vargas; Oppitz, Sheila Jacques; Silva, Thalisson Francisco Finamôr da; Santos, Sinéia Neujahr Dos; Bruno, Rúbia Soares; Filha, Valdete Alves Valentins Dos Santos; Cóser, Pedro Luis

2016-01-01

To establish reference intervals for cognitive potential P300 latency using tone burst stimuli. This study involved 28 participants aged between 18 and 59 years. P300 recordings were performed using a two-channel device (Masbe, Contronic). Electrode placement was as follows: Fpz (ground electrode), Cz (active electrode), M1 and M2 (reference electrodes). Intensity corresponded to 80 dB HL and frequent and rare stimulus frequencies to 1,000Hz and 2,000Hz, respectively. Stimuli were delivered binaurally. Mean age of participants was 35 years. Average P300 latency was 305ms. Maximum acceptable P300 latency values of 362.5ms (305 + 2SD 28.75) were determined for adults aged 18 to 59 years using the protocol described. Estabelecer valores de referência para a latência do potencial cognitivo P300 com estímulos tone burst. Participaram do estudo 28 indivíduos entre 18 e 59 anos. O registro do P300 foi realizado no equipamento Masbe da marca Contronic. Os eletrodos foram fixados nas posições Fpz (eletrodo terra), Cz (eletrodo ativo), M1 e M2 (eletrodos referência). A intensidade foi de 80 dB NA. A frequência do estímulo frequente foi de 1.000Hz e a do estímulo raro de 2.000Hz. Os estímulos foram apresentados na forma binaural. A média de idade dos indivíduos foi de 35 anos. A média de latência para P300 de 305ms. Usando o protocolo descrito, o valor máximo de latência aceitáveis para P300 foram de 362,5ms (305 + 2DP 28,75) na faixa etária do adulto de 18 a 59 anos.

Identification of phlebotomine sand flies using one MALDI-TOF MS reference database and two mass spectrometer systems

Czech Academy of Sciences Publication Activity Database

Mathis, A.; Depaquit, J.; Dvořák, V.; Tuten, H.; Banuls, A.-L.; Halada, Petr; Zapata, S.; Lehrter, V.; Hlaváčková, K.; Prudhomme, J.; Volf, P.; Sereno, D.; Kaufmann, Ch.; Pflueger, V.; Schaffner, F.

2015-01-01

Roč. 8, MAY 2015 (2015) ISSN 1756-3305 R&D Projects: GA ČR(CZ) GA15-04329S Institutional support: RVO:61388971 Keywords : Arthropod identification * Bruker * Centralized reference database Subject RIV: CE - Biochemistry Impact factor: 3.234, year: 2015

Studies of influence of high temperature preannealing on oxygen precipitation in CZ Si wafers

Czech Academy of Sciences Publication Activity Database

Meduňa, M.; Caha, O.; Buršík, Jiří

2012-01-01

Roč. 348, č. 1 (2012), s. 53-59 ISSN 0022-0248 R&D Projects: GA ČR(CZ) GA202/09/1013 Institutional research plan: CEZ:AV0Z20410507 Keywords : impurities * point defects * precipitates Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.552, year: 2012

Evaluation of reference genes for reverse transcription quantitative real-time PCR (RT-qPCR) studies in Silene vulgaris considering the method of cDNA preparation

Czech Academy of Sciences Publication Activity Database

Koloušková, Pavla; Stone, James D.; Štorchová, Helena

2017-01-01

Roč. 12, č. 8 (2017), č. článku e0183470. E-ISSN 1932-6203 R&D Projects: GA MŠk(CZ) LH15075; GA ČR(CZ) GA16-09220S Institutional support: RVO:61389030 Keywords : CYTOPLASMIC MALE -STERILITY * SUITABLE REFERENCE GENES * INTERNAL CONTROL GENES Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Plant sciences, botany Impact factor: 2.806, year: 2016

Study on grown-in defects in CZ-Si by positron annihilation

International Nuclear Information System (INIS)

Nakagawa, S.; Hori, F.; Oshima, R.

2004-01-01

In order to study the nature of grown-in microdefects of a silicon wafer taken from a czochralski-grown single crystal (CZ-Si) in which ring oxidation-induced stacking faults (ring-OSF) are formed after oxidation heat treatment, positron annihilation coincidence Doppler broadening experiments (CDB) have been performed. Vacancy-type defects were detected in the central region of a wafer of an as-grown crystal, and they were changed with annealing. It was confirmed that different types of defects were formed in the regions of outside and inside of the ring-OSF. (orig.)

EFSA Panel on food contact materials, enzymes, flavourings and processing aids (CEF); Scientific Opinion on Flavouring Group Evaluation 23, Revision 2 (FGE.23Rev2): Aliphatic, alicyclic and aromatic ethers including anisole derivatives from chemical groups 15, 16, 22, 26 and 30

DEFF Research Database (Denmark)

Larsen, John Christian; Nørby, Karin Kristiane; Beltoft, Vibe Meister

The European Food Safety Authority (EFSA) asked the Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids (the Panel) to provide scientific advice to the Commission on the implications for human health of chemically defined flavouring substances used in or on foodstuffs...... in the Member States. In particular, the Panel was requested to evaluate 19 flavouring substances in the Flavouring Group Evaluation 23, Revision 2 (FGE.23Rev2), using the Procedure as referred to in the Commission Regulation (EC) No 1565/2000. These 19 flavouring substances belong to chemical groups 15, 16, 22......-no: 03.022] Industry has informed that it occurs as a mixture of E- & Z-isomers, however, the composition of the mixture has to be specified. Two of the flavouring substances are classified into structural class I, seven are classified into structural class II and 10 are classified into structural class...

Kyselý déšť stále s námi – zdroje, mechanismy, účinky, minulost a budoucnost

Czech Academy of Sciences Publication Activity Database

Hruška, J.; Kopáček, Jiří

2005-01-01

Roč. 12, č. 5 (2005), s. 4-18 ISSN 1213-3396. [ Acid Rain 2005. International Conference on Acid Deposition /7./. Prague, 12.06.2005-17.06.2005] R&D Projects: GA ČR(CZ) GA526/03/0058; GA ČR(CZ) GA206/03/1583; GA MŽP(CZ) 1D/1/29/04 Grant - others:EC(XE) EURO-LIMPACS GOCE-CT-2003-505540 Institutional research plan: CEZ:AV0Z60170517 Keywords : water and soil acid ification * nitrogen-saturation * forest Subject RIV: DJ - Water Pollution ; Quality

In celebration of the 60th anniversary proceedings of the KSME 2005 spring annual meeting

International Nuclear Information System (INIS)

2005-01-01

This proceedings contains articles of 2005 spring annual meeting of the Korean Society of Mechanical Engineers. It was held on May 25-27, 2005 in Busan, Korea. This proceedings are comprised of 21 sessions. The main subject titles of session are as follows: nondestructive evaluation, fatigue evaluation, computation mechanics, CAE design, vibration, instrumentation, fabrication, optimum design, heat exchanger, fluid mechanics, nano fluid, plasma, heat transfer analysis, energy (Yi, J. H.)

Technostress and the Reference Librarian.

Science.gov (United States)

Kupersmith, John

1992-01-01

Defines "technostress" as the stress experienced by reference librarians who must constantly deal with the demands of new information technology and the changes they produce in the work place. Discussion includes suggested ways in which both organizations and individuals can work to reduce stress. (27 references) (LAE)

Eole 2005; Eole 2005

Energy Technology Data Exchange (ETDEWEB)

NONE

1997-10-01

The Eole 2005 programme is a french development programme of wind power plants joined up to the power distribution system. It has been launched in february 1996 by the ministry of Industry. The aim is to install in France a power of 250 to 500 Mw in wind power from now until 2005. Electricite de France is implied in other kind of renewable energies: hydroelectric power, biomass, solar energy, and geothermal energy. (N.C.)

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to a combination of lycopene, proanthocyanidins, vitamin C, vitamin E, selenium and beta-carotene and contribution to normal collagen formation (ID 1669) and protection, of the skin from UV-induced damage (ID 1669) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to a combination of lycopene, proanthocyanidins, vitamin C, vitamin E, selenium and beta-carotene and contribution to normal collagen formation and protection of the skin from UV-induced damage. The scientific substantiation is based on the information provided by the Member States...... in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent that is the subject of the health claim is a combination of lycopene, proanthocyanidins, vitamin C, vitamin E, selenium, and beta-carotene. The Panel...... considers that the combination of lycopene, proanthocyanidins, vitamin C, vitamin E, selenium, and beta-carotene is sufficiently characterised....

GlyEFSA NDA Panel (EFSA Panel on Dietetic Product s, Nutrition and Allergies), 20 13 . Scientific Opinion on the substantiation of a health claim related to glycaemic carbohydrates and recovery of normal muscle function (contraction) after stren uous exercise pursuant to Article 13(5) of Regulation

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Aptonia, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of France, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA) was asked to deliver an opinion on the scientific...... substantiation of a health claim related to glycaemic carbohydrates and recovery of normal muscle function (contraction) after strenuous exercise. The food constituent, glycaemic carbohydrates, which is the subject of the health claim, is sufficiently characterised in relation to the claimed effect. Recovery...... to the recovery of normal skeletal muscle function (contraction), the Panel concludes that a cause and effect relationship has been established between the consumption of glycaemic carbohydrates and recovery of normal muscle function (contraction) after strenuous exercise....

Body Parameters of Czech Breastfed Children Compared to the Czech References and WHO Growth Standards

Czech Academy of Sciences Publication Activity Database

Riedlová, J.; Vignerová, J.; Paulová, M.; Musil, V.; Brabec, Marek; Schneidrová, D.

2017-01-01

Roč. 44, č. 7 (2017), s. 593-599 ISSN 0301-4460 Grant - others:GA MZd(CZ) NS9974 Institutional support: RVO:67985807 Keywords : breastfed children * Czech reference * Growth charts * WHO growth standards Subject RIV: BB - Applied Statistics, Operational Research OBOR OECD: Statistics and probability Impact factor: 1.240, year: 2016

Analysis of impact of Internet marketing on visits of the Lenovo Blog CZ

OpenAIRE

Ruskovská, Petra

2013-01-01

The thesis analyses the impact of Internet marketing tools on visits of the Lenovo Blog CZ, the official blog of the computer manufacturer Lenovo. It focuses evolution of visits of the blog over time, on the impact of social networks and Facebook posts paid promotion on visits. Finally, it observes to what extent the Internet marketing tools impact overall visits of the blog over the observed time.

Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex

Czech Academy of Sciences Publication Activity Database

Libusová, Lenka; Sulimenko, Tetyana; Sulimenko, Vadym; Janisch, R.; Hozák, Pavel; Dráber, Pavel

2005-01-01

Roč. 225, 3-4 (2005), s. 157-167 ISSN 0033-183X R&D Projects: GA MŠk(CZ) LN00A026; GA AV ČR(CZ) IBS5052301; GA ČR(CZ) GA304/04/1386; GA AV ČR(CZ) KSK5020115 Institutional research plan: CEZ:AV0Z50520514 Keywords : antibody * beta-tubulin * cortex Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.573, year: 2005

An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques

Czech Academy of Sciences Publication Activity Database

Kizek, René; Masařík, Michal; Kramer, Karl J.; Potěšil, David; Bailey, M.; Howard, John A.; Klejdus, Bořivoj; Mikelová, Radka; Adam, V.; Trnková, Libuše; Jelen, František

2005-01-01

Roč. 381, č. 6 (2005), s. 1167-1178 ISSN 1618-2642 R&D Projects: GA ČR(CZ) GP525/04/P132; GA ČR(CZ) GA203/02/0422; GA MŠk(CZ) LN00A081; GA AV ČR(CZ) IAA1163201 Institutional research plan: CEZ:AV0Z50040507 Keywords : avidin * biotin * avidin- biotin technology Subject RIV: BO - Biophysics Impact factor: 2.695, year: 2005

Ring-Substituted Benzohydroxamic Acids: 1H, 13C and 15N NMR Spectra and NHOH Proton Exchange

Czech Academy of Sciences Publication Activity Database

Schraml, Jan; Tkadlecová, M.; Pataridis, S.; Soukupová, Ludmila; Blechta, Vratislav; Roithová, Jana; Exner, Otto

2005-01-01

Roč. 43, č. 7 (2005), s. 535-542 ISSN 0749-1581 R&D Projects: GA ČR(CZ) GA203/03/1566; GA AV ČR(CZ) IAA4072605; GA AV ČR(CZ) IAA4072005; GA MŠk(CZ) LB98233 Institutional research plan: CEZ:AV0Z40720504 Keywords : proton exchange * substituent effects * chemical shifts Subject RIV: CC - Organic Chemistry Impact factor: 1.553, year: 2005

Asparagine, valine, and threonine in the third extracellular loop of muscarinic receptor have essential roles in the positive cooperativity of strychnine-like allosteric modulators

Czech Academy of Sciences Publication Activity Database

Jakubík, Jan; Krejčí, Alena; Doležal, Vladimír

2005-01-01

Roč. 313, č. 2 (2005), s. 688-696 ISSN 0022-3565 R&D Projects: GA ČR(CZ) GA309/02/1331; GA ČR(CZ) GP305/02/D090; GA AV ČR(CZ) IAA5011306; GA MŠk(CZ) LC554 Institutional research plan: CEZ:AV0Z50110509 Keywords : muscarinic receptors * positive cooperativity * mutation Subject RIV: ED - Physiology Impact factor: 4.098, year: 2005

Photovoltaic Small Molecules of TPA(FxBT-T-Cz)3: Tuning Open-Circuit Voltage over 1.0 V for Their Organic Solar Cells by Increasing Fluorine Substitution.

Science.gov (United States)

Wang, Qiong; Duan, Linrui; Tao, Qiang; Peng, Wenhong; Chen, Jianhua; Tan, Hua; Yang, Renqiang; Zhu, Weiguo

2016-11-09

To simultaneously improve both open-circuit voltage (V oc ) and short-circuit current density (J sc ) for organic solar cells, a novel D(A-π-Ar) 3 type of photovoltaic small molecules of TPA(F x BT-T-3Cz) 3 was designed and synthesized, which contain central triphenylamine (TPA), terminal carbazole (Cz), armed fluorine-substituted benzothiadiazole (F x BT, where x = 1 or 2), and bridged thiophene (T) units. A narrowed ultraviolet-visible absorption and a decreasing highest occupied molecular orbital energy level were observed from TPA(F 1 BT-T-3Cz) 3 to TPA(F 2 BT-T-3Cz) 3 with increasing fluorine substitution. However, the TPA(F 2 BT-T-3Cz) 3 /PC 71 BM-based solar devices showed a rising V oc of 1.01 V and an enhanced J sc of 10.84 mA cm -2 as well as a comparable power conversion efficiency of 4.81% in comparison to the TPA(F 1 BT-T-3Cz) 3 /PC 71 BM-based devices. Furthermore, in comparison to the parent TPA(BT-T-3Cz) 3 molecule without fluorine substitution, the fluorine-substituted TPA(F x BT-T-3Cz) 3 molecules exhibited significantly incremental V oc and J sc values in their bulk heterojunction organic solar cells, owing to fluorine incorporation in the electron-deficient benzothiadiazole unit.

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to carbohydrate solutions and maintenance of physical performance during endurance exercise pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from the British Specialist Nutrition Association Ltd, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of the United Kingdom, the EFSA Panel on Dietetic Products, Nutrition and Allergies...... (NDA) was asked to deliver an opinion on the scientific substantiation of a health claim related to carbohydrate solutions and maintenance of physical performance during endurance exercise. The food, which is proposed by the applicant to be the subject of the health claim, is “carbohydrate solutions...... of physical performance during endurance exercise as compared to “water–electrolyte solutions” cannot be established on the basis of the information provided. Maintenance of physical performance during endurance exercise is a beneficial physiological effect. Three meta-analyses of human intervention studies...

Uncertainties in the Dutch Reference Projections. Background information for the report 'Reference Projections Energy and Emissions 2005-2020'

International Nuclear Information System (INIS)

Seebregts, A.J.; Gijsen, A.

2005-09-01

The Dutch targets for greenhouse gases, ammonia and non-methane VOCs will likely be met in 2010 according to our calculations from an uncertainty analysis in the framework of the project on Reference Projections for energy, climate and acidifying emissions. However, it is unlikely that the targets for sulphur dioxide and nitrogen oxide will be attained This study distinguished between sources of uncertainty in the input variables of the Reference Projections. These sources were quantified with the help of the 'Guidance for Uncertainty Assessment and Communication' and 'expert judgement'. With the aid of a statistical Monte Carlo analysis, margins and probability distributions were determined for the most important outcomes of the Reference Projections. These probability distributions led, for example, to several statements being made on the chances of meeting certain targets. The use of 'Guidance for Uncertainty Assessment and Communication' was also evaluated [nl

Development of a qualitative real-time PCR method to detect 19 targets for identification of genetically modified organisms.

Science.gov (United States)

Peng, Cheng; Wang, Pengfei; Xu, Xiaoli; Wang, Xiaofu; Wei, Wei; Chen, Xiaoyun; Xu, Junfeng

2016-01-01

As the amount of commercially available genetically modified organisms (GMOs) grows recent years, the diversity of target sequences for molecular detection techniques are eagerly needed. Considered as the gold standard for GMO analysis, the real-time PCR technology was optimized to produce a high-throughput GMO screening method. With this method we can detect 19 transgenic targets. The specificity of the assays was demonstrated to be 100 % by the specific amplification of DNA derived from reference material from 20 genetically modified crops and 4 non modified crops. Furthermore, most assays showed a very sensitive detection, reaching the limit of ten copies. The 19 assays are the most frequently used genetic elements present in GM crops and theoretically enable the screening of the known GMO described in Chinese markets. Easy to use, fast and cost efficient, this method approach fits the purpose of GMO testing laboratories.

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to β-hydroxy β-methylbutyrate monohydrate (HMB) alone or in combination with α-ketoisocaproic acid (KIC) and reduction of muscle tissue damage during exercise (ID 1577, , 1584), increase in lean body mass (ID 1579, 1582, 1583), increase in muscle strength (ID 1578, 1583, 1587), increase in endurance performance (ID 1580, 1581), skeletal muscle tissue repair (ID 1586) and faster recovery from muscle fatigue after exercise (ID 1576, 1585) pursuant to Article 13

DEFF Research Database (Denmark)

Tetens, Inge

Following a request from the European Commission, the Panel on Dietetic Products, Nutrition and Allergies was asked to provide a scientific opinion on a list of health claims pursuant to Article 13 of Regulation (EC) No 1924/2006. This opinion addresses the scientific substantiation of health...... and faster recovery from muscle fatigue after exercise. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly from stakeholders. The food constituent...

Polyunsaturated fatty acids of marine origin upregulate mitochondrial biogenesis and induce beta - oxidation in white fat

Czech Academy of Sciences Publication Activity Database

Flachs, Pavel; Horáková, Olga; Brauner, Petr; Rossmeisl, Martin; Pecina, Petr; Franssen-van Hal, N.; Růžičková, Jana; Šponarová, Jana; Drahota, Zdeněk; Vlček, Čestmír; Keijer, J.; Houštěk, Josef; Kopecký, Jan

2005-01-01

Roč. 48, č. 11 (2005), s. 2365-2375 ISSN 0012-186X R&D Projects: GA AV ČR(CZ) KJB5011410; GA AV ČR(CZ) KJB5011303; GA ČR(CZ) GA303/05/2580; GA ČR(CZ) GA303/03/0749; GA MŠk(CZ) LN00A079; GA MŠk(CZ) 1M0520 Grant - others:GA-(NO) Pronova Biocare; GA-(NL) Ministry of Agriculture, Nature Management and Food Control Institutional research plan: CEZ:AV0Z50110509 Keywords : fish oil * PGC-1 * adipose tissue Subject RIV: ED - Physiology Impact factor: 5.337, year: 2005

Swiss electricity statistics 2005; Schweizerische Elektrizitaetsstatistik 2005/Statistique suisse de l'electricite 2005

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-07-01

This comprehensive report made by the Swiss Federal Office of Energy (SFOE) presents the statistics for 2005 on electricity production and usage in Switzerland for the year 2005. First of all, an overview of Switzerland's electricity supply in 2005 is presented. Details are noted of the proportions generated by different sources including nuclear, hydro-power, storage schemes and thermal power stations as well as energy transfer with neighbouring countries. A second chapter takes a look at the balance of imports and exports with illustrative flow diagrams along with tables for total figures from 1950 through to 2005. For the summer and winter periods, figures from 1995 to 2005 are presented. The third chapter examines the production of electricity in the various types of power stations and the developments over the years 1950 to 2005, whereby, for example, statistics on regional generation and power station type are looked at. The fourth chapter looks at electricity consumption in various sectors from 1983 to 2005 and compares the figures with international data. The fifth chapter looks at generation, consumption and loading on particular days and chapter six considers energy exchange with Switzerland's neighbours. Chapter seven takes a look at possibilities for extending generation facilities in the period up to 2012.

Determination of copper binding in Pseudomonas putida CZ1 by chemical modifications and X-ray absorption spectroscopy.

Science.gov (United States)

Chen, XinCai; Shi, JiYan; Chen, YingXu; Xu, XiangHua; Chen, LiTao; Wang, Hui; Hu, TianDou

2007-03-01

Previously performed studies have shown that Pseudomonas putida CZ1 biomass can bind an appreciable amount of Cu(II) and Zn(II) ions from aqueous solutions. The mechanisms of Cu- and Zn-binding by P. putida CZ1 were ascertained by chemical modifications of the biomass followed by Fourier transform infrared and X-ray absorption spectroscopic analyses of the living or nonliving cells. A dramatic decrease in Cu(II)- and Zn(II)-binding resulted after acidic methanol esterification of the nonliving cells, indicating that carboxyl functional groups play an important role in the binding of metal to the biomaterial. X-ray absorption spectroscopy was used to determine the speciation of Cu ions bound by living and nonliving cells, as well as to elucidate which functional groups were involved in binding of the Cu ions. The X-ray absorption near-edge structure spectra analysis showed that the majority of the Cu was bound in both samples as Cu(II). The fitting results of Cu K-edge extended X-ray absorption fine structure spectra showed that N/O ligands dominated in living and nonliving cells. Therefore, by combining different techniques, our results indicate that carboxyl functional groups are the major ligands responsible for the metal binding in P. putida CZ1.

Object recognition by implicit invariants

Czech Academy of Sciences Publication Activity Database

Flusser, Jan; Kautsky, J.; Šroubek, Filip

2007-01-01

Roč. 2007, č. 4673 (2007), s. 856-863 ISSN 0302-9743. [Computer Analysis of Images and Patterns. Vienna, 27.08.2007-29.08.2007] R&D Projects: GA MŠk 1M0572 Institutional research plan: CEZ:AV0Z10750506 Keywords : Invariants * implicit invariants * moments * orthogonal polynomials * nonlinear object deformation Subject RIV: JD - Computer Applications, Robotics Impact factor: 0.402, year: 2005 http:// staff .utia.cas.cz/sroubekf/papers/CAIP_07.pdf

Nuclear topography and expression of the BCR/ABL fusion gene and its protein level influenced by cell differentiation and RNA interference

Czech Academy of Sciences Publication Activity Database

Bártová, Eva; Harničarová, Andrea; Pacherník, Jiří; Kozubek, Stanislav

2005-01-01

Roč. 29, č. 8 (2005), s. 901-913 ISSN 0145-2126 R&D Projects: GA AV ČR(CZ) 1QS500040508; GA ČR(CZ) GA202/04/0907; GA MZd NC6987; GA AV ČR(CZ) IAA5004306; GA MŠk(CZ) LC535 Institutional research plan: CEZ:AV0Z50040507 Keywords : BCR /ABL fusion gene * chromatin arrangement * gene expression Subject RIV: BO - Biophysics Impact factor: 2.372, year: 2005

Restoration of a river backwater and its influence on fish assemblage

Czech Academy of Sciences Publication Activity Database

Hohausová, Eva; Jurajda, Pavel

2005-01-01

Roč. 50, č. 10 (2005), s. 473-482 ISSN 1212-1819 R&D Projects: GA ČR(CZ) GA206/93/0734; GA ČR(CZ) GA206/97/0162; GA AV ČR(CZ) IAA6087503 Institutional research plan: CEZ:AV0Z60170517 Keywords : rehabilitation and restoration * long-term monitoring * colonisation Subject RIV: EH - Ecology, Behaviour Impact factor: 0.254, year: 2005 http://www.cazv.cz/attachments/CJAS_50_473_482.pdf

Crusting susceptibility in some allic Colombian soils

International Nuclear Information System (INIS)

Arias, Dora M; Madero E E; Amezquita E

2001-01-01

Many lab methods were used: dry and water soil aggregates stability, instability index and erosion index and their results were related with soil characteristics like texture, Fe and Al oxides and organic matter. Soil samples collected within 0-2.5 and 2.5-5 cm of the soil surface came from terrains with many kinds of both forest and savanna intervened systems. Those results were analyzed like a completely randomized designed. It was found that significative changes in oxides content could increase soil-crusting susceptibility unless soil humus was up to was up to 4%. In this sense, pastures or its rotation with rice and leguminous offer a best alternative for intervening these natural systems. Intensive land husbandry or monocultures with low stubble soil incorporation caused an increase in physical instability at the top of soil. Dry soil stability test and instability index were most adequate for these soils

EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA); Scientific Opinion on the substantiation of health claims related to sodium and potassium salts of citric acid and maintenance of normal bone (ID 330) pursuant to Article 13(1) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

claims in relation to sodium and potassium salts of citric acid and maintenance of normal bone. The scientific substantiation is based on the information provided by the Member States in the consolidated list of Article 13 health claims and references that EFSA has received from Member States or directly...... from stakeholders. The food constituents that are the subject of the health claim are sodium and potassium salts of citric acid. The Panel considers that sodium and potassium salts of citric acid are sufficiently characterised. The claimed effect is “acid-base balance and bone health”. The target...... using a higher dose of potassium citrate did not show an effect on bone mineral density. On the basis of the data presented, the Panel concludes that a cause and effect relationship has not been established between the dietary intake of potassium or sodium salts of citric acid and maintenance of normal...

Expedice NAMAK 2005

Czech Academy of Sciences Publication Activity Database

Filippi, Michal; Bruthans, J.; Svoboda, T.

2006-01-01

Roč. 25, - (2006), s. 47-51 ISSN 1211-8397 R&D Projects: GA AV ČR(CZ) KJB301110501 Institutional research plan: CEZ:AV0Z30130516 Keywords : salt karst * Iran * caves * expedition Namak Subject RIV: DB - Geology ; Mineralogy

Minimal residual disease as the target for immunotherapy and gene therapy of cancer (Review)

Czech Academy of Sciences Publication Activity Database

Bubeník, Jan; Šímová, Jana

2005-01-01

Roč. 14, č. 5 (2005), s. 1377-1380 ISSN 1021-335X R&D Projects: GA MZd(CZ) NR7807; GA MZd(CZ) NR8004; GA ČR(CZ) GA301/04/0492 Institutional research plan: CEZ:AV0Z50520514 Keywords : residual tumour disease * gene therapy * cytokines Subject RIV: EC - Immunology Impact factor: 1.572, year: 2005

Broadband Photometry Of The Potentially Asteroid 277475 (2005 WK4) and Corrected 52762 (1998 MT24) Colors.

Science.gov (United States)

Hicks, M.; Buratt, B.; Carcione, A.; Borlase, R.

2013-08-01

The Near-Earth Object (NEO) 277475 (2005 WK4) was discovered by the Siding Spring Survey (MPEC 2005-W79) on November 27, 2005. With a Minimum Orbit Intersection Distance (MOID) of 0.004 AU and absolute magnitude H_V=20.1 mag, this object has been designated a Potentially Hazardous Asteroid (PHA) by the Minor Planet Center. The asteroid made an Earth close-approach of 0.021 AU on August 09.2, 2013 and was extensively imaged by the JPL Planetary Radar Team ( http://www.jpl.nasa.gov/news/news.php?release=2013-254 ).

Co přináší srovnání registru ALERT-CZ s dřívějším pilotním registrem akutního infarktu myokardu?

Czech Academy of Sciences Publication Activity Database

Peleška, Jan; Reissigová, Jindra; Tomečková, Marie; Grünfeldová, H.; Janský, P.; Monhart, Z.; Vojáček, J.; Widimský, P.; Zvárová, Jana

2010-01-01

Roč. 52, č. 9 (2010), s. 573-573 ISSN 0010-8650. [Konference České společnosti pro hypertenzi /27./, Konference pracovní skupiny Preventivní kardiologie ČKS /19./, Konference pracovní skupiny Srdeční selhání ČKS /15./. 07.10.2010-09.10.2010, Mariánské Lázně] R&D Projects: GA MŠk(CZ) 1M06014 Institutional research plan: CEZ:AV0Z10300504 Keywords : registr akutního infarktu myokardu * rizikové faktory infarktu myokardu * PCI a akutní farmakoterapie infarktu myokardu Subject RIV: IN - Informatics, Computer Science

Factors affecting the 27K DNA methylation pattern in asthmatic and healthy children from locations with various environments

Czech Academy of Sciences Publication Activity Database

Rössnerová, Andrea; Tulupová, Elena; Tabashidze, Nana; Schmuczerová, Jana; Dostál, Miroslav; Rössner ml., Pavel; Gmuender, H.; Šrám, Radim

2013-01-01

Roč. 741-742, January (2013), s. 18-26 ISSN 0027-5107 R&D Projects: GA ČR GAP503/11/0084; GA ČR GA13-13458S; GA MŽP(CZ) SP/1B3/8/08 Institutional support: RVO:68378041 Keywords : 27K BeadChip * air pollution * bronchial asthma Subject RIV: DN - Health Impact of the Environment Quality Impact factor: 4.440, year: 2013

AIP – Aterogenní index plazmy jako významný prediktor kardiovaskulárního rizika: od výzkumu do praxe

Czech Academy of Sciences Publication Activity Database

Dobiášová, Milada

2006-01-01

Roč. 52, č. 1 (2006), s. 64-71 ISSN 0042-773X. [Pokroky ve farmakoterapii 2005 pro lékaře v praxi. Zlín, 07.09.2005-09.09.2005] R&D Projects: GA MZd(CZ) NR8328; GA MZd(CZ) NA6590; GA ČR(CZ) GV306/96/K220 Institutional research plan: CEZ:AV0Z50110509 Keywords : AIP * Log (TG/HDL-C) * particle size of lipoproteins Subject RIV: FA - Cardiovascular Diseases incl. Cardiotharic Surgery

Reference: 272 [Arabidopsis Phenome Database[Archive

Lifescience Database Archive (English)

Full Text Available m L et al. 2005 Oct. Plant J. 44(1):114-27. The specification of epidermal (L1) identity occurs early during...s within the suspensor. Markers for L1 identity, ACR4 and ATML1, are not expressed in homozygous mutant embr...ng seedlings showed a specific loss of epidermal cell identity within large portions of the cotyledons. In a

Antibiotic resistance marker genes as environmental pollutants in GMO-pristine agricultural soils in Austria.

Science.gov (United States)

Woegerbauer, Markus; Zeinzinger, Josef; Gottsberger, Richard Alexander; Pascher, Kathrin; Hufnagl, Peter; Indra, Alexander; Fuchs, Reinhard; Hofrichter, Johannes; Kopacka, Ian; Korschineck, Irina; Schleicher, Corina; Schwarz, Michael; Steinwider, Johann; Springer, Burkhard; Allerberger, Franz; Nielsen, Kaare M; Fuchs, Klemens

2015-11-01

Antibiotic resistance genes may be considered as environmental pollutants if anthropogenic emission and manipulations increase their prevalence above usually occurring background levels. The prevalence of aph(3')-IIa/nptII and aph(3')-IIIa/nptIII - frequent marker genes in plant biotechnology conferring resistance to certain aminoglycosides - was determined in Austrian soils from 100 maize and potato fields not yet exposed to but eligible for GMO crop cultivation. Total soil DNA extracts were analysed by nptII/nptIII-specific TaqMan real time PCR. Of all fields 6% were positive for nptII (median: 150 copies/g soil; range: 31-856) and 85% for nptIII (1190 copies/g soil; 13-61600). The copy-number deduced prevalence of nptIII carriers was 14-fold higher compared to nptII. Of the cultivable kanamycin-resistant soil bacteria 1.8% (95% confidence interval: 0-3.3%) were positive for nptIII, none for nptII (0-0.8%). The nptII-load of the studied soils was low rendering nptII a typical candidate as environmental pollutant upon anthropogenic release into these ecosystems. Copyright © 2015 Elsevier Ltd. All rights reserved.

Swiss energy statistics 2005; Schweizerische Gesamtenergiestatistik 2005/Statistique globale suisse de l'energie 2005

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-07-01

This comprehensive report by the Swiss Federal Office of Energy (SFOE) presents statistics on energy production and consumption in Switzerland in 2005. Facts and figures are presented in tables and diagrams. First of all, a general overview of Swiss energy consumption is presented that includes details on the shares taken by the various energy carriers involved and their development during the period reviewed. The report also includes graphical representations of energy usage in various sectors such as households, trade and industry, transport and the services sector. Also, economic data on energy consumption is presented. A second chapter takes a look at energy flows from producers to consumers and presents an energy balance for Switzerland in the form of tables and an energy-flow diagram. The individual energy sources and the import, export and storage of energy carriers are discussed as is the conversion between various forms and categories of energy. Details on the consumption of energy, its growth over the years up to 2005 and energy use in various sectors are presented. Also, the Swiss energy balance with reference to the use of renewable forms of energy such as solar energy, biomass, wastes and ambient heat is discussed and figures are presented on the contribution of renewables to heating and the generation of electrical power. The third chapter provides data on the individual energy carriers and the final chapter looks at economical and ecological aspects. An appendix provides information on the methodology used in collecting the statistics and on data available in the Swiss cantons.

A genetic algorithm-based search for parametric reform alternatives for the Turkish pension system : 2005-2060

OpenAIRE

Alparslan, Mustafa Artun

2005-01-01

Ankara : The Department of Economics, Bilkent Univ., 2005. Thesis (Master's) -- Bilkent University, 2005. Includes bibliographical references leaves 54-55. In this thesis, we search for parametric reform alternatives so as to achieve a balance in the long term accumulated difference between pension expenditures and revenues of the SSI, the largest pension fund in Turkey, from 2005 to 2060. The projected worker-retiree composition of the pension system is allowed to change al...

[Soil-transmitted helminth trends and prevalence in La Virgen, Colombia 1995-2005].

Science.gov (United States)

Fernández-Niño, Julián A; Reyes-Harker, Patricia; Moncada-Alvarez, Ligia I; López, Myriam C; Cháves, María Del Pilar; Knudson, Angélica; Ariza, Yoseth

2007-01-01

Describing soil-transmitted helminthiasis prevalence and trends in children aged less than 15 in the village of La Virgen, Cundinamarca. Three non-random surveys were carried out on school-children aged 0 to 15 years. Intestinal parasitism was determined In the three cross-sectional studies by direct examination of fecal samples and modified Ritchie-Frick concentration method. Intestinal parasitism distribution was analysed and the trend during 1995-2005 described. The prevalence of intestinal parasitism in children aged less than 5 increased from 62,5 % in 1995 to 66,7 % in 2001 and to 69 % in 2005; soil-transmitted helminthiasis prevalence in this age group was 37,5 % in 1995, 23,6 % in 2001 and 27,6 % in 2005. The prevalence of intestinal parasitism for children aged over 5 increased from 86,2 % in 1995 to 89,1 % in 2005; soil-transmitted helminthiasis prevalence was 62,9 % in 1995, 39,8 % in 2001 and 23,9 % in 2005. Soil-transmitted helminthiasis was endemic and presented high prevalence during the study period. Effective control measures are needed to prevent intestinal parasitism in pre-school and schoolchildren.

Ligand binding to the human MT2 melatonin receptor: The role of residues in transmembrane domains 3, 6, and 7

Czech Academy of Sciences Publication Activity Database

Mazna, Petr; Berka, K.; Jelínková, Irena; Balík, Aleš; Svoboda, Petr; Obšilová, Veronika; Obšil, T.; Teisinger, Jan

2005-01-01

Roč. 332, č. 3 (2005), s. 726-734 ISSN 0006-291X R&D Projects: GA AV ČR(CZ) KJB5011308; GA ČR(CZ) GA309/02/1479; GA ČR(CZ) GA204/03/0714; GA ČR(CZ) GA309/04/0496 Institutional research plan: CEZ:AV0Z5011922 Keywords : MT2 melatonin receptor * homology modeling * binding study Subject RIV: FR - Pharmacology ; Medidal Chemistry Impact factor: 3.000, year: 2005

Assembly factors of F1FO-ATP synthase across genomes

Czech Academy of Sciences Publication Activity Database

Pícková, Andrea; Potocký, Martin; Houštěk, Josef

2005-01-01

Roč. 59, č. 3 (2005), s. 393-402 ISSN 0887-3585 R&D Projects: GA MŠk(CZ) 1M0520; GA MZd(CZ) NR7790 Grant - others:GA UK(CZ) 12/2002; GA UK(CZ) 11/2004; EC Framework Programme(XE) LSHM-CT-2004-503116 Institutional research plan: CEZ:AV0Z50110509 Keywords : assembly * ATP synthase * phylogenetic and sequence analysis Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition Impact factor: 4.684, year: 2005

Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities

Czech Academy of Sciences Publication Activity Database

Lukášová, Emilie; Kořistek, Z.; Falk, Martin; Kozubek, Stanislav; Grigoryev, S.; Kozubek, Michal; Ondřej, Vladan; Kroupová, I.

2005-01-01

Roč. 77, č. 1 (2005), s. 1-12 ISSN 0741-5400 R&D Projects: GA MZd NC6987; GA AV ČR(CZ) IAA1065203; GA AV ČR(CZ) KSK5052113; GA AV ČR(CZ) IBS5004010; GA ČR(CZ) GA202/02/0804; GA MŠk ME 565 Institutional research plan: CEZ:AV0Z50040507 Keywords : human granulocytes differentiation * chromatin condensation * heterochromatin Subject RIV: BO - Biophysics Impact factor: 4.627, year: 2005

EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies), 2014. Scientific Opinion on the substantiation of a health claim related to non-digestible carbohydrates and reduction of post-prandial glycaemic responses pursuant to Article 13(5) of Regulation (EC) No 1924/2006

DEFF Research Database (Denmark)

Tetens, Inge

Following an application from Beneo-Orafti SA, Sensus BV and Cosucra-Groupe Warcoing SA, submitted for authorisation of a health claim pursuant to Article 13(5) of Regulation (EC) No 1924/2006 via the Competent Authority of Belgium, the EFSA Panel on Dietetic Products, Nutrition and Allergies (NDA...... not contribute to post-prandial glycaemia. This opinion applies to non-digestible carbohydrates (e.g. non-starch polysaccharides, resistant oligosaccharides and resistant starch) which should replace sugars in foods or beverages in order to obtain the claimed effect. The Panel considers that the food constituent......-prandial glycaemic responses might be a beneficial physiological effect. In weighing the evidence, the Panel took into account that consumption of non-digestible carbohydrates results in reduced post-prandial blood glucose (and insulinaemic) responses compared with the consumption of sugars on a weight...

An advanced reference genome of Trifolium subterraneum L. reveals genes related to agronomic performance

Czech Academy of Sciences Publication Activity Database

Kaur, P.; Bayer, P.E.; Milec, Zbyněk; Vrána, Jan; Yuan, Y.; Appels, R.; Edwards, D.; Batley, J.; Nichols, P.; Erskine, W.; Doležel, Jaroslav

2017-01-01

Roč. 15, č. 8 (2017), s. 1034-1046 ISSN 1467-7644 R&D Projects: GA MŠk(CZ) LO1204; GA ČR GBP501/12/G090 Institutional support: RVO:61389030 Keywords : advanced reference assembly * BioNano * forage legumes * gene expression * Legume comparative genomics * transcriptome Subject RIV: EB - Genetics ; Molecular Biology OBOR OECD: Environmental biotechnology Impact factor: 7.443, year: 2016

Enova results report 2005; Enova resultatrapport 2005

Energy Technology Data Exchange (ETDEWEB)

NONE

2006-07-01

In 2005 Enova entered into agreements with 186 projects that together provided an energy result of 2 TWh. The result is almost evenly divided between projects providing higher energy production and projects that reduce energy use. The energy production consists of 585 GWh in new wind power and 409 GWh in new heat energy in 2005. Overall for the period 2001 to 2005, Enova contracted for energy results of well over 6.6 TWh. Enova is doing well in relation to the objectives set for its activities.

k0-INAA for APM samples collected in period of June 2004 - March 2005 and some marine certified reference materials

International Nuclear Information System (INIS)

Dung, Ho Manh; Vu, Cao Dong; Y, Truong; Sy, Nguyen Thi

2006-01-01

The airborne particulate matter (APM) samples have been collected in 2004 using two types of polycarbonate membrane filter PM 2.5 and PM 2.5-10 at two sites of industrial (Ho Chi Mihn City) and rural (Dateh) regions in south of Vietnam. Three marine certified reference materials have been selected to establish a k0-NAA procedure for marine samples. The concentration of trace multi-element in the samples has been determined by the k 0 -INAA procedure using K o -DALAT software developed in Dalat NRI. About 28 elements in 224 APM samples collected at two areas of Dateh and HCMC of Vietnam in period from June, 2004 to March, 2005 were presented in report. The statistical analysis was applied to the data set to investigate the pollution source at sampling sites. The results proved that the k 0 -NAA on the Dalat research reactor is a reliable and effective analytical technique for characterization of trace multi-element in APM and marine samples for air and marine environmental pollution study in Vietnam. (author)

Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method

Czech Academy of Sciences Publication Activity Database

Vaisocherová, Hana; Zítová, Alice; Lachmanová, Markéta; Štepánek, J.; Králíková, Šárka; Liboska, Radek; Rejman, Dominik; Rosenberg, Ivan; Homola, Jiří

2006-01-01

Roč. 82, č. 4 (2006), s. 394-398 ISSN 0006-3525. [European Conference on the Spectroscopy of Biological Molecules - ECSBM 2005 /11./. Aschaffenburg, 03.09.2005-08.09.2005] R&D Projects: GA ČR(CZ) GA303/03/0249; GA ČR(CZ) GA102/03/0633; GA ČR(CZ) GA202/05/0628 Institutional research plan: CEZ:AV0Z20670512; CEZ:AV0Z40550506 Keywords : surface plasmon resonance * biosensors * optical sensors Subject RIV: BO - Biophysics Impact factor: 2.480, year: 2006

Adhesion and growth of vascular endothelial and smooth muscle cells on artificial vascular prostheses and protein-coated surfaces

Czech Academy of Sciences Publication Activity Database

Chlupáč, Jaroslav; Filová, Elena; Brynda, Eduard; Remy-Zolghadri, M.; Bačáková, Lucie

2005-01-01

Roč. 3, č. S1 (2005), S8-S9 ISSN 1214-021X. [Cells /6./ - Biological days /18./. 24.10.2005-26.10.2005, České Budějovice] R&D Projects: GA MŠk(CZ) 1M0510; GA AV ČR(CZ) IAA5011301; GA AV ČR(CZ) IAA4050202 Institutional research plan: CEZ:AV0Z50110509 Keywords : tissue engineering * bioartificial vessel * dynamic culture system * hemodynamic bench * fibronectin * collagen * endothelialization * sex-related differences Subject RIV: EI - Biotechnology ; Bionics

Allosteric modulation of the nicotinic acetylcholine receptor by physostigmine

Czech Academy of Sciences Publication Activity Database

Svobodová, Lucie; Krůšek, Jan; Hendrych, Tomáš; Vyskočil, František

2005-01-01

Roč. 1048, - (2005), s. 355-358 ISSN 0077-8923 R&D Projects: GA ČR(CZ) GA202/02/1213; GA ČR(CZ) GA305/02/1333; GA ČR(CZ) GD305/03/H148 Institutional research plan: CEZ:AV0Z5011922 Keywords : nicotinic ACh receptor * serine * desensitization Subject RIV: ED - Physiology Impact factor: 1.971, year: 2005

Circadian rhythmicity in AVP secretion and GABAergic synaptic transmission in the rat suprachiasmatic nucleus

Czech Academy of Sciences Publication Activity Database

Kretschmannová, Karla; Svobodová, Irena; Balík, Aleš; Mazna, Petr; Zemková, Hana

2005-01-01

Roč. 1048, - (2005), s. 103-115 ISSN 0077-8923 R&D Projects: GA ČR(CZ) GA309/02/1519; GA AV ČR(CZ) IAA5011103; GA AV ČR(CZ) IAA5011408 Institutional research plan: CEZ:AV0Z5011922 Keywords : circadian rhythms * suprachiasmatic nucleus * melatonin Subject RIV: FH - Neuro logy Impact factor: 1.971, year: 2005

Sex differences in connexin-43 expression in left ventricles of aging rats

Czech Academy of Sciences Publication Activity Database

Tribulová, N.; Dupont, E.; Soukup, Tomáš; Okruhlicová, L.; Severs, N. J.

2005-01-01

Roč. 54, č. 6 (2005), s. 705-708 ISSN 0862-8408 R&D Projects: GA ČR(CZ) GA304/05/0327 Grant - others:MYORES(XE) 511978; CZ-SK(CZ) 02-2004-05 Institutional research plan: CEZ:AV0Z50110509 Keywords : connexin-43 * rat cardiomyocytes * male and female specificity Subject RIV: ED - Physiology Impact factor: 1.806, year: 2005

DNA hybridization on membrane-modified carbon electrodes

Czech Academy of Sciences Publication Activity Database

Kouřilová, Alena; Babkina, S. S.; Cahová, Kateřina; Havran, Luděk; Jelen, František; Paleček, Emil; Fojta, Miroslav

2005-01-01

Roč. 38, - (2005), s. 2493-2507 ISSN 0003-2719 R&D Projects: GA MPO(CZ) 1H-PK/42; GA AV ČR(CZ) IAA4004402; GA AV ČR(CZ) IBS5004355 Institutional research plan: CEZ:AV0Z50040507 Keywords : DNA hybridization * electrochemical DNA sensor * nitrocellulose membrane Subject RIV: BO - Biophysics Impact factor: 1.036, year: 2005

Simulation of cerebrospinal fluid transport

Czech Academy of Sciences Publication Activity Database

Otáhal, Jakub; Štěpáník, Z.; Kaczmarská, A.; Maršík, František; Brož, Z.; Otáhal, S.

2007-01-01

Roč. 38, 11-12 (2007), s. 802-809 ISSN 0965-9978 Grant - others:GA UK(CZ) 112/2005; GA UK(CZ) 114/2005; GA ČR(CZ) GA106/03/0958 Program:GA Institutional research plan: CEZ:AV0Z50110509; CEZ:AV0Z20760514 Keywords : cerebrospinal fluid * pulsation * mathematical modeling Subject RIV: BO - Biophysics Impact factor: 0.529, year: 2007

Postnatal caffeine exposure: effects on motor skills and locomotor activity during ontogenesis

Czech Academy of Sciences Publication Activity Database

Tchekalarova, Jana; Kubová, Hana; Mareš, Pavel

2005-01-01

Roč. 160, č. 1 (2005), s. 99-106 ISSN 0166-4328 R&D Projects: GA ČR(CZ) GA304/04/0464 Grant - others:CZ-BG(CZ) Joint research project (2002-2004); NATO(XX) Science Fellowships Programme (2001-2002) Institutional research plan: CEZ:AV0Z5011922 Keywords : caffeine * ontogenesis * motor skills Subject RIV: FH - Neurology Impact factor: 2.865, year: 2005

Action of granulopoiesis-stimulating cytokines rhG-CSF, rhGM-CSF, and rmGM-CSF on murine hematopoietic progenitor cells for granulocytes and macrophages (GM-CFC)

Czech Academy of Sciences Publication Activity Database

Hofer, Michal; Vacek, Antonín; Weiterová, Lenka

2005-01-01

Roč. 54, - (2005), s. 207-213 ISSN 0862-8408 R&D Projects: GA AV ČR(CZ) IBS5004009; GA AV ČR(CZ) KSK5011112; GA ČR(CZ) GP305/03/D050 Institutional research plan: CEZ:AV0Z50040507 Keywords : murine hematopoiesis * GM-CFC * rhG- CSF Subject RIV: BO - Biophysics Impact factor: 1.806, year: 2005

Dynamic modification of microorganisms by pyrenebutanoate for fluorometric detection in capillary zone electrophoresis

Czech Academy of Sciences Publication Activity Database

Horká, Marie; Růžička, F.; Holá, V.; Šlais, Karel

2005-01-01

Roč. 26, č. 3 (2005), s. 548-555 ISSN 0173-0835 R&D Projects: GA AV ČR(CZ) IBS4031201; GA ČR(CZ) GA203/02/1447; GA AV ČR(CZ) IAA4031302 Institutional research plan: CEZ:AV0Z40310501 Keywords : CZE * microorganisms * fluorometric detection Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.850, year: 2005

DNA adducts of the enantiomers of the Pt(II) complexes of the ahaz ligand (ahaz=3-aminohexahydroazepine) and recognition of these adducts by HMG domain proteins

Czech Academy of Sciences Publication Activity Database

Malina, Jaroslav; Vojtíšková, Marie; Brabec, Viktor; Diakos, Connie I.; Hambley, Trevor W.

2005-01-01

Roč. 332, č. 4 (2005), s. 1034-1041 ISSN 0006-291X R&D Projects: GA ČR(CZ) GA305/05/2030; GA AV ČR(CZ) IAA5004101; GA AV ČR(CZ) IBS5004107 Institutional research plan: CEZ:AV0Z50040507 Keywords : cisplatin * anticancer * enantiomers Subject RIV: BO - Biophysics Impact factor: 3.000, year: 2005

Luminometric method for screening retroviral protease inhibitors

Czech Academy of Sciences Publication Activity Database

Horáková, D.; Rumlová, Michaela; Pichová, Iva; Ruml, Tomáš

2005-01-01

Roč. 345, č. 1 (2005), s. 96-101 ISSN 0003-2697 R&D Projects: GA AV ČR(CZ) IAA4055304; GA MŠk(CZ) 1M0508; GA MŠk(CZ) 1M0520 Institutional research plan: CEZ:AV0Z40550506 Keywords : retroviral protease * inhibitors * luminescent assay Subject RIV: CE - Biochemistry Impact factor: 2.670, year: 2005

Genome size variation in Macaronesian Angiosperms: Forty Percent of Canarian Endemic Flora Completed

Czech Academy of Sciences Publication Activity Database

Suda, Jan; Kyncl, Tomáš; Jarolímová, Vlasta

2005-01-01

Roč. 252, 3-4 (2005), s. 215-238 ISSN 0378-2697 R&D Projects: GA ČR(CZ) GA206/00/1445; GA ČR(CZ) GA206/04/0081; GA AV ČR(CZ) KSK6005114 Institutional research plan: CEZ:AV0Z60050516 Keywords : genome size * cytometry * Macaronesia Subject RIV: EF - Botanics Impact factor: 1.421, year: 2005

EFSA Panel on Dietetic Products, Nutrition and Allerg ies (NDA) ; Guidance on the scientific requirements for health claims related to appetite ratings, weight management, and blood glucose concentrations

DEFF Research Database (Denmark)

Tetens, Inge

The European Food Safety Authority (EFSA) asked the Panel on Dietetic Products, Nutrition and Allergies (NDA) to draft guidance on scientific requirements for health claims related to appetite ratings, weight management, and blood glucose concentrations. This guidance has been drawn from scientific...... and studies/outcome measures which are acceptable. Rather, it presents examples drawn from evaluations already carried out in order to illustrate the approach of the Panel, as well as some examples which are currently under consideration within ongoing evaluations. A draft of this guidance document, endorsed...

Stream.cz a jeho originální seriálová tvorba

OpenAIRE

Vašíčková, Dorota

2017-01-01

In general, the rise of the internet has brought many changes of production and distribution into the audio-visual industry. These changes triggered the development of specific internet portals that offer video content. The thesis focuses on the current development of internet televisions and especially on a particular platform and enriches the current theoretical list of forms with a specific example of a Czech internet television. The case study of the Czech internet television Stream.cz de...

Corneal rat-to-mouse xenotransplantation and the effects of anti-CD4 or anti-CD8 treatment on cytokine and nitric oxide production

Czech Academy of Sciences Publication Activity Database

Pindjáková, Jana; Vítová, Andrea; Krulová, Magdalena; Zajícová, Alena; Filipec, M.; Holáň, Vladimír

2005-01-01

Roč. 18, č. 7 (2005), s. 854-862 ISSN 0934-0874 R&D Projects: GA MZd(CZ) NR7816; GA MZd(CZ) NI7531; GA ČR GP310/02/D162; GA ČR(CZ) GD310/03/H147; GA MŠk(CZ) ME 300; GA AV ČR KSK5020115 Institutional research plan: CEZ:AV0Z5052915 Keywords : corneal xenotransplantation * cytokines * nitric oxide Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 1.797, year: 2005

Tissue-specific cytochrome c oxidase assembly defects due to mutations in SCO2 and SURF1

Czech Academy of Sciences Publication Activity Database

Stibůrek, L.; Veselá, K.; Hansíková, H.; Pecina, Petr; Tesařová, M.; Černá, L.; Houštěk, Josef; Zeman, J.

2005-01-01

Roč. 392, č. 3 (2005), s. 625-632 ISSN 0264-6021 R&D Projects: GA ČR(CZ) GA303/03/0749; GA MŠk(CZ) 1M0520; GA ČR(CZ) GP303/03/D132 Grant - others:GA UK(CZ) 17/2004/C; Priority 1(XE) LSHMCT-2004-503116 Institutional research plan: CEZ:AV0Z50110509 Keywords : assembly pathway * cytochrome c oxidase * SURF1 Subject RIV: FB - Endocrinology, Diabetology, Metabolism, Nutrition Impact factor: 4.224, year: 2005

Lead Isotopic Composition in Biogenic Certified Reference Materials Determined by Different ICP-based Mass Spectrometric Techniques

Czech Academy of Sciences Publication Activity Database

Ďurišová, J.; Ackerman, L.; Strnad, L.; Chrastný, V.; Borovička, Jan

2015-01-01

Roč. 39, č. 2 (2015), s. 209-220 ISSN 1639-4488 R&D Projects: GA ČR(CZ) GAP504/11/0484 Institutional support: RVO:61389005 Keywords : Pb isotopes * Pb-206 * Pb-207 * Pb-208 * ICP-MS * certified reference materials * vegetation Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 2.885, year: 2015

Direct linkage of mitochondrial genome variation to risk factors for type 2 diabetes in conplastic strains

Czech Academy of Sciences Publication Activity Database

Pravenec, Michal; Hyakukoku, M.; Houštěk, Josef; Zídek, Václav; Landa, Vladimír; Mlejnek, Petr; Mikšík, Ivan; Mothejzíková-Dudová, Kristýna; Pecina, Petr; Vrbacký, Marek; Drahota, Zdeněk; Vojtíšková, Alena; Mráček, Tomáš; Kazdová, L.; Oliyarnyk, O.; Wang, Ji.; Ho, Ch.; Qi, N.; Sugimoto, K.; Kurtz, T.

2007-01-01

Roč. 17, č. 9 (2007), s. 1319-1326 ISSN 1088-9051 R&D Projects: GA MŠk(CZ) 1M0520; GA ČR(CZ) GA301/06/0028; GA ČR GA303/07/0781 Grant - others:GA UK(CZ) 24/2005; GA UK(CZ) 26/2005; National Institutes of Health(US) HL35018; National Institutes of Health(US) HL56028; National Institutes of Health(US) HL63709; EURATOOLS(XE) LSHG-CT-2005-019015 Institutional research plan: CEZ:AV0Z50110509 Source of funding: R - rámcový projekt EK Keywords : mitochondrial genome * conplastic strains * risk factors for type 2 diabetes Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 11.224, year: 2007

Potencial evocado auditivo de longa latência-P300 em indivíduos normais: valor do registro simultâneo em Fz e Cz P300-long-latency auditory evoked potential in normal hearing subjects: simultaneous recording value in Fz and Cz

Directory of Open Access Journals (Sweden)

Josilene Luciene Duarte

2009-04-01

Full Text Available O P300 é um Potencial Evocado Auditivo denominado potencial endógeno por refletir o uso funcional que o indivíduo faz do estímulo auditivo, sendo altamente dependente das habilidades cognitivas, entre elas atenção e discriminação auditiva. É um procedimento de avaliação objetiva, mas que depende da experiência do avaliador em detectar os picos das ondas, sendo importante a utilização de métodos de registro que facilitem a análise da presença de resposta e a interpretação dos resultados. OBJETIVO: Analisar o Potencial Evocado Auditivo de Longa Latência-P300 obtido com a utilização de dois eletrodos ativos posicionados em Fz e Cz. MATERIAIS E MÉTODOS: Participaram deste estudo 33 indivíduos de ambos os gêneros com idade entre 7 e 34 anos, audição normal e sem fator de risco para problemas mentais. RESULTADOS: Os resultados demonstraram que não houve diferença estatisticamente significante para a latência de N2 e P3 e amplitude do P3 quando analisado o gênero e nem correlação com a idade dos indivíduos. Houve forte correlação destas medidas com o posicionamento dos eletrodos em Fz e Cz. CONCLUSÃO: O posicionamento dos eletrodos ativos em Fz e Cz pode ser considerado um recurso a mais para auxiliar na análise clínica do P300.The P300 is and auditory Evoked Potential, called endogenous potential because it reflects the functional use the individual makes of the auditory stimulus, being highly dependent on cognitive skills; among them we list attention and auditory discrimination. It is a procedure of objective evaluation; however, one that depends on the examiner's experience to detect wave peaks, and it is important to use recording methods that facilitate the response presence analysis and result interpretation. AIM: to analyze the P300 Long Latency Auditory Evoked Potential obtained through the use of two active electrodes positioned on Fz and Cz. MATERIALS AND METHODS: 330 individuals from both genders and

Effect of free-radical spin trap N-tert-butyl-alpha-phenylnitrone on seizures induced in immature rats by homocysteic acid

Czech Academy of Sciences Publication Activity Database

Folbergrová, Jaroslava; Druga, Rastislav; Otáhal, Jakub; Haugvicová, Renata; Mareš, Pavel; Kubová, Hana

2005-01-01

Roč. 46, č. S6 (2005), s. 375-375 ISSN 0013-9580. [International Epilepsy Congress /26./. 28.08.2005-01.09.2005, Paris] R&D Projects: GA ČR(CZ) GA309/02/1238; GA ČR(CZ) GA309/05/2015 Keywords : free radical scavenger * epilepsy * immature rats * homocysteic acid Subject RIV: ED - Physiology

Novel modified purine bases and nucleosides: new methodologies of synthesis and biological activity

Czech Academy of Sciences Publication Activity Database

Hocek, Michal

-, č. 49 (2005), s. 29-30 ISSN 0261-3166. [International Symposium on Nucleic Acids Chemistry /4./. Fukuoka, 20.09.2005-22.09.2005] R&D Projects: GA ČR(CZ) GA203/03/0035; GA MŠk(CZ) 1M0508 Institutional research plan: CEZ:AV0Z40550506 Keywords : purines * nucleosides * synthesis Subject RIV: CC - Organic Chemistry

Effect of risperidon and haloperidol in active allothetic place avoidance (AAPA) in animal model of schizophrenia

Czech Academy of Sciences Publication Activity Database

Valeš, Karel; Bubeníková, V.; Rambousek, Lukáš; Řezáčová, Lenka; Stuchlík, Aleš

2005-01-01

Roč. 16, Suppl. 1 (2005), S95-S95 ISSN 0955-8810. [Biennial European behavioural pharmacology society meeting /11./. 09.09.2005-12.09.2005, Barcelona] R&D Projects: GA MŠk(CZ) LC554; GA MŠk(CZ) 1M0517 Institutional research plan: CEZ:AV0Z50110509 Keywords : memory Subject RIV: FH - Neuro logy

Pokroky ve fotochemii singletového kyslíku

Czech Academy of Sciences Publication Activity Database

Lang, Kamil; Mosinger, Jiří; Wagnerová, Dana Marie

2005-01-01

Roč. 99, č. 4 (2005), s. 211-221 ISSN 0009-2770 R&D Projects: GA ČR(CZ) GA203/04/0426; GA ČR(CZ) GA203/02/1483; GA ČR(CZ) GA203/02/0420 Institutional research plan: CEZ:AV0Z40320502 Keywords : photodynamic therapy * molecular-oxygen * hydrogen -peroxide Subject RIV: CA - Inorganic Chemistry Impact factor: 0.445, year: 2005

A quantitative version of Krein´s Theorem. To the memory of Vlastimil Pták

Czech Academy of Sciences Publication Activity Database

Fabian, Marián; Hájek, Petr Pavel; Montesinos, V.; Zizler, Václav

2005-01-01

Roč. 21, č. 1 (2005), s. 237-248 ISSN 0213-2230 R&D Projects: GA AV ČR(CZ) IAA1019003; GA ČR(CZ) GA201/01/1198; GA AV ČR(CZ) IAA1019205 Institutional research plan: CEZ:AV0Z10190503 Keywords : Banach spaces * weak compatness * Krein´s Theorem Subject RIV: BA - General Mathematics Impact factor: 0.855, year: 2005

Expression and potential role of fibroblast growth factor 2 and its receptors in human embryonic stem cells

Czech Academy of Sciences Publication Activity Database

Dvořák, Petr; Dvořáková, D.; Košková, S.; Vidinská, M.; Najvirtová, M.; Krekáč, D.; Hampl, Aleš

2005-01-01

Roč. 23, č. 8 (2005), s. 1200-1211 ISSN 1066-5099 R&D Projects: GA ČR(CZ) GA301/03/1122; GA ČR(CZ) GA305/05/0434; GA MŠk(CZ) LN00A065 Institutional research plan: CEZ:AV0Z50390512 Keywords : growth factor * human embryonic stem cells Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 6.094, year: 2005

Far-infrared soft-mode behavior in PbSc.sub.1/2./sub.Ta.sub.1/2./sub.O.sub.3./sub. thin films

Czech Academy of Sciences Publication Activity Database

Kamba, Stanislav; Berta, Milan; Kempa, Martin; Hlinka, Jiří; Petzelt, Jan; Brinkman, K.; Setter, N.

2005-01-01

Roč. 98, - (2005), 074103/1-074103/5 ISSN 0021-8979 R&D Projects: GA AV ČR(CZ) IAA1010213; GA ČR(CZ) GA202/04/0993; GA MŠk(CZ) OC 525.20 Institutional research plan: CEZ:AV0Z10100520 Keywords : relaxor ferroelectric * slim films * ferroelectric soft mode * central mode Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 2.498, year: 2005

Patterning of hydrogenated microcrystalline silicon growth by magnetic field

Czech Academy of Sciences Publication Activity Database

Fejfar, Antonín; Stuchlík, Jiří; Mates, Tomáš; Ledinský, Martin; Honda, Shinya; Kočka, Jan

2005-01-01

Roč. 87, č. 1 (2005), 011901/1-011901/3 ISSN 0003-6951 R&D Projects: GA AV ČR(CZ) IAA1010316; GA AV ČR(CZ) IAA1010413; GA ČR(CZ) GD202/05/H003 Institutional research plan: CEZ:AV0Z10100521 Keywords : hydrogenated microcrystalline silicon * magnetic field growth Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 4.127, year: 2005

Common vole (Microtus arvalis) population sex ratio: biases and process variation

Czech Academy of Sciences Publication Activity Database

Bryja, Josef; Nesvadbová, Jiřina; Heroldová, Marta; Jánová, Eva; Losík, J.; Trebatická, L.; Tkadlec, Emil

2005-01-01

Roč. 83, č. 11 (2005), s. 1391-1399 ISSN 0008-4301 R&D Projects: GA ČR(CZ) GA524/01/1316; GA ČR(CZ) GP206/02/P068; GA ČR(CZ) GA206/04/2003 Institutional research plan: CEZ:AV0Z60930519 Keywords : common vole * population sex ratio Subject RIV: EH - Ecology, Behaviour Impact factor: 1.175, year: 2005

Immunotherapeutic efficacy of vaccines generated by fusion of dendritic cells and HPV16-associated tumour cells

Czech Academy of Sciences Publication Activity Database

Šímová, Jana; Bubeník, Jan; Bieblová, Jana; Indrová, Marie; Jandlová, Táňa

2005-01-01

Roč. 51, č. 1 (2005), s. 19-24 ISSN 0015-5500 R&D Projects: GA AV ČR(CZ) IAA5052203; GA ČR(CZ) GA301/04/0492 Grant - others:Liga proti rakovině, Praha(CZ) - Institutional research plan: CEZ:AV0Z50520514 Keywords : HPV16 * DC-tumour hybrids * immunotherapy Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.719, year: 2005

System zones in capillary zone electrophoresis: Moving boundaries caused by freely migrating hydrogen ions

Czech Academy of Sciences Publication Activity Database

Beckers, J. L.; Boček, Petr

2005-01-01

Roč. 26, č. 2 (2005), s. 446-452 ISSN 0173-0835 R&D Projects: GA ČR(CZ) GA203/02/0023; GA AV ČR(CZ) IAA4031401; GA AV ČR(CZ) IAA4031103 Institutional research plan: CEZ:AV0Z40310501 Keywords : capillary zone electrophoresis * system zone s Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 3.850, year: 2005

Long-term effect of elevated CO2 on spatial differentiation of ribulose-1,5-bisphosphate carboxylase/oxygenase activity in Norway spruce canopy

Czech Academy of Sciences Publication Activity Database

Hrstka, M.; Urban, Otmar; Marek, Michal V.

2005-01-01

Roč. 43, č. 2 (2005), s. 211-216 ISSN 0300-3604 R&D Projects: GA MŠk(CZ) LN00A141; GA MŽP(CZ) SM/640/18/03; GA MŠk(CZ) OC E21.001 Institutional research plan: CEZ:AV0Z6087904 Keywords : down-regulation * photosynthesis * sun and shade needles * vertical profile Subject RIV: ED - Physiology Impact factor: 0.810, year: 2005

Near-Critical Extraction of ß-Sitosterol and Scopoletin from Stinging Nettle Roots

Czech Academy of Sciences Publication Activity Database

Sajfrtová, Marie; Sovová, Helena; Opletal, L.; Bártlová, Milena

2005-01-01

Roč. 35, č. 2 (2005), s. 111-118 ISSN 0896-8446 R&D Projects: GA ČR(CZ) GA203/01/0550; GA ČR(CZ) GD203/03/H140; GA AV ČR(CZ) IAA4072102 Institutional research plan: CEZ:AV0Z40720504 Keywords : carbon dioxide * ß-sitosterol * scopoletin Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 2.144, year: 2005

Preparation of lead iodide as input material for X-Ray detectors

Czech Academy of Sciences Publication Activity Database

Matuchová, Marie; Procházková, Olga; Žďánský, Karel; Zavadil, Jiří; Maixner, J.

2005-01-01

Roč. 480-481, - (2005), s. 477-481 ISSN 0255-5476 R&D Projects: GA ČR(CZ) GA102/03/0379; GA ČR(CZ) GA102/04/0959; GA ČR(CZ) GA102/01/1338 Institutional research plan: CEZ:AV0Z20670512 Keywords : lead compounds * purification * photoluminescence * zone melting Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 0.399, year: 2005

Determination of toxicity of spoil substrates after brown coal mining using a laboratory reproduction test with .i.Enchytraeus crypticus./i. (Oligochaeta)

Czech Academy of Sciences Publication Activity Database

Frouz, Jan; Krištůfek, Václav; Bastl, J.; Kalčík, Jiří; Vaňková, H.

2005-01-01

Roč. 162, 1-2 (2005), s. 37-47 ISSN 0049-6979 R&D Projects: GA ČR(CZ) GA526/01/1055; GA ČR(CZ) GA526/03/1259; GA AV ČR(CZ) 1QS600220501 Institutional research plan: CEZ:AV0Z60660521 Keywords : brown coal mining * chronic toxicity test * enchytraeidae Subject RIV: EH - Ecology, Behaviour Impact factor: 1.258, year: 2005

Marketingový plán společnosti PHYTO CZ, s.r.o.

OpenAIRE

Začal, Libor

2011-01-01

The Goal of the Master's thesis is to create a marketing plan for a specific company. The first part contains chapters that capture the theoretical foundations needed to build a marketing plan. The practical part deals with the Phyto CZ, Ltd. Company which operates in the production and sale of food supplements. It contains the steps needed to develop a marketing plan. First, the company's marketing mix a selected elements of situational analysis are analysed, with the results of a questionna...

ICALEPS 2005: closing session and ICALEPCS2005 prizes

CERN Multimedia

Maximilien Brice

2005-01-01

ICALEPCS 2005, the tenth International Conference on Accelerator and Large Experimental Physics Control Systems, will be held in Geneva, Switzerland, 10-14 Oct. 2005 at the International Conference Center Geneva (CICG). ICALEPCS 2005 thus falls in the year that UNESCO has declared the "World Year of Physics". ICALEPCS covers all aspects of control and operation of Experimental Physics facilities such as particle accelerators, particle detectors, optical telescopes, radio telescopes, nuclear fusion facilities like Tokamaks, nuclear reactors, lasers, etc ....

Marketingová analýza úspěšnosti projektu ZaNázory.cz

OpenAIRE

Pohan, Lukáš

2012-01-01

The bachelor thesis analyses the ZaNázory.cz project, its various parts and its success as well as its target audience. The analysis of the project is based on experience from the making of this project. Other field of interest of this thesis is to evaluate the success of this project in present and its future forecasts and compares the estimated target audience with real target audience at present.

Academic Training: 2005 - 2006 Academic Training Programme

CERN Multimedia

Françoise Benz

2005-01-01

1st Term : 03.10. 2005 - 16.12.2005 LECTURE SERIES Einstein's impact on the physics of the Twentieth Century by N. Straumann / Univ. Zürich, CH 3, 4, 5, 6, 7 October 11:00 -1200 - Auditorium, Bldg 500 Surviving in space: the challenges of a manned mission to Mars by L. S. Pinsky / Univ. Houston, USA 26, 27, 28 October 11:00-12:00 - Auditorium, Bldg 500 Climate change and challenges for the environment by C. Schluechter, Univ. Ber, CH 14, 15, 16 November 11:00-12:00 - TH Auditorium, Bldg 4, 3rd Floor Introduction to Cryogenic Engineering by Goran Perinic, CERN- AT 5, 6, 7, 8, 9 December 11:00-12:00 - Auditorium, Bldg 500 Predicting natural catastrophes by E. Okal, Northwestern Univ. , USA 12, 13, 14, 15, 16 December 11:00-12:00 - Auditorium, Bldg 500 The lectures are open to all those interested, without application. The abstract of the lectures, as well as any change to the above information (title, dates, time, place etc.) will be published in the CERN bulletin, the WWW, and by Notices before ea...

The activity of inducible nitric oxide synthase in rejected skin xenografts is selectively inhibited by a factor produced by grafted cells

Czech Academy of Sciences Publication Activity Database

Holáň, Vladimír; Pindjáková, Jana; Zajícová, Alena; Krulová, Magdalena; Železná, Blanka; Matoušek, Petr; Svoboda, Petr

2005-01-01

Roč. 12, č. 3 (2005), s. 227-234 ISSN 0908-665X R&D Projects: GA MZd(CZ) NR7816; GA ČR(CZ) GP310/02/D162; GA ČR(CZ) GD310/03/H147; GA MŠk(CZ) ME 300; GA AV ČR KSK5020115 Institutional research plan: CEZ:AV0Z5052915; CEZ:AV0Z50110509 Keywords : inducible nitric oxide synthase production * nitric oxide * suppressive molecule Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.114, year: 2005

Diurnal dynamics of photosynthetic parameters of Norway spruce trees cultivated under ambient and elevated CO2: the reasons of midday depression in CO2 assimilation

Czech Academy of Sciences Publication Activity Database

Špunda, V.; Kalina, J.; Urban, Otmar; Luis, V.C.; Sibisse, I.; Puértolas, J.; Šprtová, Miroslava; Marek, Michal V.

2005-01-01

Roč. 168, č. 5 (2005), s. 1371-1381 ISSN 0168-9452 R&D Projects: GA MŠk(CZ) LN00A141; GA MŠk(CZ) 1P04OCE21.001; GA AV ČR(CZ) IBS6087005; GA MŽP SM/640/18/03 Grant - others:EU(CZ) HPRI-CT-2002-00197 Institutional research plan: CEZ:AV0Z60870520 Keywords : daily course of photosynthesis * elevated CO2 * dynamics of acclimation depression of photosynthesis Subject RIV: ED - Physiology Impact factor: 1.605, year: 2005

Preface - The 27th International Conference on Phenomena in Ionized Gase

NARCIS (Netherlands)

Kroesen, G.M.W.; Kroesen, G.M.W.

2006-01-01

The 27th International Conference on Phenomena in Ionized Gases (ICPIG) was held in the conference resort of NH Koningshof in Veldhoven, near Eindhoven, The Netherlands, 17–22 July 2005. ICPIG is an important biennial event at which academics and industrialists working in low-temperature plasma

STUDIES REFERED TO SERVICES IN THE 2005-2010 ENANPADS: A BIBLIOMETRIC SURVEY

Directory of Open Access Journals (Sweden)

José Antônio Menezes Varanda

2014-12-01

Full Text Available This bibliometric survey concerns articles published in the Marketing Academic Division of the EnANPADs with the word service(s in the title from 2005 to 2010. The research selected 51 articles with the following characteristics: number of authors per article, their respective institutions of affiliation, the frequency of attendance to the events, the methodological approaches, the means and ends of the researches and the data collection tools used. Data were tabulated and processed with descriptive statistics, and results showed that services are a relevant theme in Marketing research, either with a central or peripheral position. In the conclusions, five alerts were thrown, including the imbalance of research between private and public institutions and the geographic concentration of the participating institutions. Future studies are suggested.

Characterization and optimization of GMO-based gels with long term release for intraarticular administration.

Science.gov (United States)

Réeff, J; Gaignaux, A; Goole, J; Siepmann, J; Siepmann, F; Jerome, C; Thomassin, J M; De Vriese, C; Amighi, K

2013-07-15

Osteoarthritis is characterized by slow degenerative processes in the articular cartilage within synovial joints. It could be interesting to develop a sustained-release formulation that could be effective on both pain/inflammation and restoration of mechanical integrity of the joint. Recently, an injectable system based on glycerol monooleate (GMO), containing clonidine as a model hydrophilic analgesic/anti-inflammatory drug and hyaluronic acid as a viscoelastic scaffold, showed promising potential as a biodegradable and biocompatible preparation to sustain the drug activity. However, drug release from the system is relatively fast (complete within 1 week) and the underlying drug release mechanisms not fully understood. The aims of this study were: (i) to significantly improve this type of local controlled drug delivery system by further sustaining clonidine release, and (ii) to elucidate the underlying mass transport mechanisms. The addition of FDA-approved inactive ingredients such as sodium oleate or purified soybean oil was found to be highly effective. The release rate could be substantially reduced (e.g., 50% release after 10 days), due to the increased hydrophobicity of the systems, resulting in slower and reduced water uptake and reduced drug mobility. Interestingly, Fick's second law of diffusion could be used to quantitatively describe drug release. Copyright © 2013. Published by Elsevier B.V.

Capillary network in slow and fast muscles and in oxidative and glycolytic muscle fibres

Czech Academy of Sciences Publication Activity Database

Čebašek, V.; Kubínová, Lucie; Ribarič, S.; Eržen, I.

2005-01-01

Roč. 24, March (2005), s. 51-58 ISSN 1580-3139 Grant - others:SI-CZ(CZ) KONTAKT 19/2005 Institutional research plan: CEZ:AV0Z50110509 Keywords : capillaries * skeletal muscle fibre s-oxidative and glycolytic * stereology Subject RIV: EA - Cell Biology

Hadron collider physics 2005. Proceedings

International Nuclear Information System (INIS)

Campanelli, M.; Clark, A.; Wu, X.

2006-01-01

The Hadron Collider Physics Symposia (HCP) are a new series of conferences that follow the merger of the Hadron Collider Conferences with the LHC Symposia series, with the goal of maximizing the shared experience of the Tevatron and LHC communities. This book gathers the proceedings of the first symposium, HCP2005, and reviews the state of the art in the key physics directions of experimental hadron collider research: - QCD physics - precision electroweak physics - c-, b-, and t-quark physics - physics beyond the Standard Model - heavy ion physics The present volume will serve as a reference for everyone working in the field of accelerator-based high-energy physics. (orig.)

Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005

DEFF Research Database (Denmark)

Klein, Jörn; Hussain, Manzoor; Ahmad, Munir

2007-01-01

Background According to the World Reference Laboratory for FMD, a new subtype of FMDV serotype A was detected in Iran in 2005. This subtype was designated A/IRN/2005, and rapidly spread throughout Iran and moved westwards into Saudi Arabia and Turkey where it was initially detected from August 2005....../IRN/2005 sublineage with the A22 sublineage. Potential recombination events have been detected in parts of the genome region coding for the non-structural proteins of FMDV. In addition, amino acid substitutions have been detected in the deduced VP1 protein sequence, potentially related to clinical...... their evolutionary starting point in the A22 sublineage. It can be assumed that, due to the quasispecies structure of FMDV populations and the error-prone replication process, advantageous mutations in a changed environment have been fixed and lead to the occurrence of the new A/IRN/2005 sublineage. Together...

Effect of hydrogen passivation on polycrystalline silicon thin films

Czech Academy of Sciences Publication Activity Database

Honda, Shinya; Mates, Tomáš; Ledinský, Martin; Oswald, Jiří; Fejfar, Antonín; Kočka, Jan; Yamazaki, T.; Uraoka, Y.; Fuyuki, T.

2005-01-01

Roč. 487, - (2005), s. 152-156 ISSN 0040-6090 R&D Projects: GA AV ČR(CZ) IAA1010316; GA AV ČR(CZ) IAA1010413; GA ČR(CZ) GD202/05/H003 Institutional research plan: CEZ:AV0Z10100521 Keywords : hydrogen passivation * polycrystalline silicon * photoluminescence * Raman spectroscopy * Si-H 2 * hydrogen molecules Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.569, year: 2005

Models of pesticides inside cavities of molecular dimensions. A role of the guest inclusion in the dechlorination process

Czech Academy of Sciences Publication Activity Database

Hromadová, Magdaléna; Pospíšil, Lubomír; Fanelli, N.; Giannarelli, S.

2005-01-01

Roč. 21, č. 5 (2005), s. 1923-1930 ISSN 0743-7463 R&D Projects: GA ČR(CZ) GP203/02/P082; GA ČR(CZ) GA203/03/0821 Grant - others:GA MŠk(CZ) 1P04OCD31.001 Institutional research plan: CEZ:AV0Z40400503 Keywords : beta-cyclodextrin * electrochemical reduction * gamma-cyclodextrin * alpha-cyclodextrin Subject RIV: CG - Electrochemistry Impact factor: 3.705, year: 2005

Determination of picogram quantities of oligodeoxynucleotides by stripping voltammetry at mercury modified graphite electrode surfaces

Czech Academy of Sciences Publication Activity Database

Hasoň, Stanislav; Jelen, František; Fojt, Lukáš; Vetterl, Vladimír

2005-01-01

Roč. 577, č. 2 (2005), s. 263-272 ISSN 0022-0728 R&D Projects: GA AV ČR IAA4004404; GA AV ČR(CZ) KJB4004305; GA AV ČR(CZ) IBS5004107; GA ČR(CZ) GA203/02/0422 Institutional research plan: CEZ:AV0Z50040507 Keywords : pyrolitic graphite electrode * glassy carbon electrode * mercury film electrodes Subject RIV: BO - Biophysics Impact factor: 2.223, year: 2005

Faecal nitrogen: a potential indicator of red and roe deer diet quality in forest habitats

Czech Academy of Sciences Publication Activity Database

Kamler, Jiří; Homolka, Miloslav

2005-01-01

Roč. 54, 1-2 (2005), s. 89-98 ISSN 0139-7893 R&D Projects: GA ČR(CZ) GA206/99/D053; GA AV ČR(CZ) IBS6093003 Institutional research plan: CEZ:AV0Z60930519 Keywords : free-living ungulates * faecal indicators * diet quality Subject RIV: EG - Zoology Impact factor: 0.585, year: 2005 http://www.ivb.cz/folia/54/1-2/89-98.pdf

Homology modeling and SAR analysis of Schistosoma japonicum cathepsin D (SjCD) with statin inhibitors identify a unique active site steric barrier with potential for the design of specific inhibitors

Czech Academy of Sciences Publication Activity Database

Caffrey, C. R.; Plachá, L.; Bařinka, Cyril; Hradilek, Martin; Dostál, Jiří; Sajid, M.; McKerrow, J. H.; Majer, P.; Konvalinka, Jan; Vondrášek, Jiří

2005-01-01

Roč. 386, č. 4 (2005), 339-349 ISSN 1431-6730 R&D Projects: GA MŠk(CZ) LC512; GA ČR(CZ) GP203/02/P095; GA AV ČR(CZ) IAA400550510 Grant - others:SFSF(US) AI 53247; NATO(XE) CLG 974914 Institutional research plan: CEZ:AV0Z4055905 Keywords : schistotoma * proteases of parasites * modelling Subject RIV: CE - Biochemistry Impact factor: 2.577, year: 2005

Lifting the fog in store-operated Ca2+ entry

Czech Academy of Sciences Publication Activity Database

Dráber, Petr; Dráberová, Lubica

2005-01-01

Roč. 26, č. 12 (2005), s. 621-624 ISSN 1471-4906 R&D Projects: GA AV ČR(CZ) IAA5052310; GA AV ČR(CZ) IBS5052201; GA MŠk(CZ) 1M0506 Institutional research plan: CEZ:AV0Z5052915; CEZ:AV0Z50520514 Keywords : plasma membrane * calcium uptake * stromal interaction molecule Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 10.174, year: 2005

Deposition of PZT Thin Films on Polymer Substrate by Means of Low Pressure Plasma Jet Systém

Czech Academy of Sciences Publication Activity Database

Deyneka, Alexander; Hubička, Zdeněk; Jastrabík, Lubomír; Čada, Martin; Virostko, Petr; Olejníček, Jiří; Suchaneck, G.; Gerlach, G.

2005-01-01

Roč. 316, - (2005), s. 157-166 ISSN 0015-0193 R&D Projects: GA AV ČR(CZ) KJB1010301; GA ČR(CZ) GP202/02/D078; GA AV ČR(CZ) KJB1010302 Institutional research plan: CEZ:AV0Z10100520; CEZ:AV0Z10100521 Keywords : ferroelectric thin films * spectral ellipsometry * hollow cathode sputtering Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 0.459, year: 2005

Catalytic Decomposition of Nitrous Oxide over Catalysts Prepared from Co/Mg-Mn/Al Hydrotalcite-like Compounds

Czech Academy of Sciences Publication Activity Database

Obalová, L.; Jirátová, Květa; Kovanda, F.; Pacultová, K.; Lacný, Z.; Mikulová, Zuzana

2005-01-01

Roč. 60, 3-4 (2005), s. 289-297 ISSN 0926-3373 R&D Projects: GA ČR(CZ) GA106/05/0366; GA ČR(CZ) GA104/04/2116; GA ČR(CZ) GA106/02/0523 Institutional research plan: CEZ:AV0Z40720504 Keywords : decomposition of nitrous oxide * hydrotalcite-like compounds Subject RIV: CI - Industrial Chemistry, Chemical Engineering Impact factor: 3.809, year: 2005

Review - Host specificity of insect herbivores in tropical forests

Czech Academy of Sciences Publication Activity Database

Novotný, Vojtěch; Basset, Y.

2005-01-01

Roč. 272, č. 1568 (2005), s. 1083-1090 ISSN 0962-8452 R&D Projects: GA AV ČR(CZ) IAA6007106; GA ČR(CZ) GD206/03/H034; GA ČR(CZ) GA206/04/0725; GA MŠk(CZ) ME 646 Grant - others:US Nationals Science Foundation(US) DEB-02-11591; Darwin Initiative for the Survival of Species(US) 162/10/030 Institutional research plan: CEZ:AV0Z50070508 Keywords : food web * herbivore guild * host plant range Subject RIV: EH - Ecology, Behaviour Impact factor: 3.510, year: 2005

Liquid damper for suppressing horizontal and vertical motions - parametric study

Czech Academy of Sciences Publication Activity Database

Pirner, Miroš; Urushadze, Shota

2007-01-01

Roč. 95, 9-11 (2007), s. 1329-1349 ISSN 0167-6105. [EACWE 4. Praha, 11.07.2005-15.07.2005] R&D Projects: GA ČR(CZ) GA103/05/2066; GA MDS (CZ) 1F45D/013/120; GA ČR(CZ) GA103/06/0099; GA AV ČR(CZ) IAA2071401 Institutional research plan: CEZ:AV0Z20710524 Keywords : effects of viscosity * rectangular container with two degrees of freedom * random excitation Subject RIV: JM - Building Engineering Impact factor: 0.959, year: 2007

Host specialization and species richness of fruit flies (Diptera : Tephritidae) in a New Guinea rain forest

Czech Academy of Sciences Publication Activity Database

Novotný, Vojtěch; Clarke, A. R.; Drew, R. A. I.; Balagawi, S.; Clifford, B.

2005-01-01

Roč. 21, - (2005), s. 67-77 ISSN 0266-4674 R&D Projects: GA AV ČR(CZ) IAA6007106; GA ČR(CZ) GD206/03/H034; GA ČR(CZ) GA206/04/0725; GA MŠk(CZ) ME 646 Grant - others:Australian Centre for International Agricultural Research(AU) CS2/1996/225; US National Science Foundation(US) DEB-02-11591 Institutional research plan: CEZ:AV0Z50070508 Keywords : beta-diversity * fruit s * herbivore communities Subject RIV: EG - Zoology Impact factor: 1.013, year: 2005

First-principles study of new series of quaternary Heusler alloys CsSrCZ (Z=Si, Ge, Sn, P, As, and Sb)

Energy Technology Data Exchange (ETDEWEB)

Bouabça, A. [Condensed Matter and Sustainable Development Laboratory (LMCDD), University of Sidi Bel-Abbes, Sidi Bel-Abbes 22000 (Algeria); Rozale, H., E-mail: hrozale@yahoo.fr [Condensed Matter and Sustainable Development Laboratory (LMCDD), University of Sidi Bel-Abbes, Sidi Bel-Abbes 22000 (Algeria); Amar, A. [Condensed Matter and Sustainable Development Laboratory (LMCDD), University of Sidi Bel-Abbes, Sidi Bel-Abbes 22000 (Algeria); Wang, X.T. [School of Physics and Electronic Engineering, Chongqing Normal University, Chongqing 400044 (China); Sayade, A. [UCCS, CNRS-UMR 8181, Université d’Artois, Faculté des Sciences Jean Perrin, Rue Jean Souvraz, SP 18, 62307 Lens Cedex (France); Chahed, A. [Condensed Matter and Sustainable Development Laboratory (LMCDD), University of Sidi Bel-Abbes, Sidi Bel-Abbes 22000 (Algeria)

2016-12-01

The structural, electronic, magnetic, and thermal properties of new quaternary Heusler alloys CsSrCZ (Z=Si, Ge, Sn, P, As, and Sb) were investigated using the full-potential linearized augmented plane wave (FPLAPW) within the generalized gradient approximation (GGA) and GGA plus modified Becke and Johnson as the exchange correlation. The results showed that all Heusler compounds were stable in Type (I) structure. The CsSrCZ (Z=Si, Ge, Sn) compounds had a nearly HM characteristic, and CsSrCZ (Z=P, As, Sb) compounds were true half-metallic (HM) ferromagnets. The strong spin polarization of p orbital for C, Si, Ge, Sn, P, As, and Sb atoms is found to be the origin of ferromagnetic. The half-metallicity is preserved up to a lattice contraction of 3.45%, 1.69%, 1.69%, 7.16%, 7.16%, and 11.2% for all six quaternary Heusler compounds. We also investigated the thermal effects using the quasi-harmonic Debye model. - Highlights: • Electronic, magnetic, and thermodynamic properties of CsSrCZ (Z=Si, Ge, Sn, P, As, and Sb) are investigated. • Until now, there have been no reports theoretical and experimental studies on d{sup 0} half-metals with quaternary structures. • The strong spin polarization of p orbital for C, Si, Ge, Sn, P, As, and Sb atoms is found to be the origin of ferromagnetic. • The half-metallicity is preserved up to a lattice contraction.

Electronics engineer's reference book

CERN Document Server

Turner, L W

1976-01-01

Electronics Engineer's Reference Book, 4th Edition is a reference book for electronic engineers that reviews the knowledge and techniques in electronics engineering and covers topics ranging from basics to materials and components, devices, circuits, measurements, and applications. This edition is comprised of 27 chapters; the first of which presents general information on electronics engineering, including terminology, mathematical equations, mathematical signs and symbols, and Greek alphabet and symbols. Attention then turns to the history of electronics; electromagnetic and nuclear radiatio

Chromatography in authenticity and traceability tests of vegetable oils and dairy products: a review

Czech Academy of Sciences Publication Activity Database

Cserháti, T.; Forgács, E.; Deyl, Zdeněk; Mikšík, Ivan

2005-01-01

Roč. 19, č. 3 (2005), s. 183-190 ISSN 0269-3879 Grant - others:CZ-HU(CZ) Cooperation programme Institutional research plan: CEZ:AV0Z50110509 Keywords : chromatography * dairy products * vegetable oils Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 1.218, year: 2005

Interaction of hydroxypropyl-beta-cyclodextrin with peptides, studied by reversed-phase thin-layer chromatography

Czech Academy of Sciences Publication Activity Database

Gere-Pászti, E.; Cserháti, T.; Forgács, E.; Deyl, Zdeněk; Mikšík, Ivan; Eckhardt, Adam; Illés, Z.

2005-01-01

Roč. 28, č. 16 (2005), s. 2619-2632 ISSN 1082-6076 Grant - others:CZ-HU(CZ) Cooperation program Institutional research plan: CEZ:AV0Z50110509 Keywords : cyclodextrin * peptides * nonlinear map Subject RIV: CB - Analytical Chemistry, Separation Impact factor: 0.814, year: 2005

Crystallography of the Sb-Te-Ni system

Czech Academy of Sciences Publication Activity Database

Laufek, F.; Drábek, M.; Skála, Roman; Císařová, I.

2005-01-01

Roč. 12, č. 2 (2005), s. 153-154 ISSN 1211-5894 Grant - others:GAUK(CZ) 43-203391 Institutional research plan: CEZ:AV0Z30130516 Keywords : crystallography * antimony * tellurium Subject RIV: DB - Geology ; Mineralogy http:// xray .cz/ms/bul2005-2/student3.pdf

Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway

Czech Academy of Sciences Publication Activity Database

Drosopoulos, K.G.; Roberts, M.L.; Čermák, Lukáš; Sasazuki, T.; Shirasawa, S.; Anděra, Ladislav; Pintzas, A.

2005-01-01

Roč. 280, č. 24 (2005), s. 22856-22867 ISSN 0021-9258 R&D Projects: GA AV ČR(CZ) KSK5020115; GA AV ČR(CZ) KJB5052407 Keywords : TRAIL * Ras * apoptosis Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 5.854, year: 2005

Towards better description of solar activity variation in the International Reference Ionosphere topside ion composition model

Czech Academy of Sciences Publication Activity Database

Truhlík, Vladimír; Bilitza, D.; Třísková, Ludmila

2015-01-01

Roč. 55, č. 8 (2015), s. 2099-2105 ISSN 0273-1177 R&D Projects: GA MŠk(CZ) LH11123 Institutional support: RVO:68378289 Keywords : ion composition * topside ionosphere * solar activity * empirical model * International Reference Ionosphere Subject RIV: BL - Plasma and Gas Discharge Physics Impact factor: 1.409, year: 2015 http://www.sciencedirect.com/science/article/pii/S027311771400489X

Off-label prescription of genetically modified organism medicines in europe: emerging conflicts of interest?

Science.gov (United States)

Schagen, Frederik H E; Hoeben, Rob C; Hospers, Geke A P

2014-10-01

Recently, the first human medicine containing a genetically modified organism (GMO medicine) was authorized for use in the European market. Just as any medicinal product, the market authorization for a GMO medicine contains a precise description of the therapeutic use for which the medicinal product is intended. Within this use, the application of the GMO medicine is permitted, without the need for the institution to obtain a specific permit. In practice, however, medicinal products are also frequently prescribed for treatment outside the registered therapeutic use, a practice that is referred to as "off-label use." While off-label use of conventional medicines is permitted and has been very useful, the off-label use of GMO medicines is not covered in the European Union (EU) legislation or guidelines and falls under each member state's national environmental legislation. This implies that in the Netherlands and most other EU member states, an environmental permit will be required for any institution that uses the GMO medicine outside the registered application(s). In the Netherlands, this permit is identical to the permits required for the execution of clinical trials involving nonregistered GMOs. The application procedure for such permit is time-consuming. This process can therefore limit the therapeutic options for medical professionals. As a consequence, desired treatment regimens could be withheld for certain patient (groups). To make future off-label use of GMO medicines permissible in a way that is acceptable for all stakeholders, regulators should adopt a proactive attitude and formulate transparent legislative procedures for this. Only then the field can maintain the public acceptance of GMO medicines, while maintaining the freedom to operate of medical professionals.

The radiographic and MRI features of gout referred as suspected soft tissue sarcoma: a review of the literature and findings from 27 cases

Energy Technology Data Exchange (ETDEWEB)

Upadhyay, Neil [Imperial College Healthcare NHS Trust, Imaging Department, London (United Kingdom); Saifuddin, Asif [The Royal National Orthopaedic Hospital NHS Trust, Department of Radiology, Stanmore, Middlesex (United Kingdom)

2014-09-24

Gout has been termed the ''great mimicker'', for its ability to resemble other pathological conditions. Whilst the typical imaging features of gout are well described, there is a relative paucity of literature describing more challenging cases of gout, where a malignant tumour has been considered in the differential diagnosis. The aim of this article is to provide an overview of the radiographic and MRI features of 27 cases of gout that were initially referred to our centre as suspected soft tissue sarcoma, alongside a review of the literature. (orig.)

The radiographic and MRI features of gout referred as suspected soft tissue sarcoma: a review of the literature and findings from 27 cases

International Nuclear Information System (INIS)

Upadhyay, Neil; Saifuddin, Asif

2015-01-01

Gout has been termed the ''great mimicker'', for its ability to resemble other pathological conditions. Whilst the typical imaging features of gout are well described, there is a relative paucity of literature describing more challenging cases of gout, where a malignant tumour has been considered in the differential diagnosis. The aim of this article is to provide an overview of the radiographic and MRI features of 27 cases of gout that were initially referred to our centre as suspected soft tissue sarcoma, alongside a review of the literature. (orig.)

Podnikatelský plán - internetový portál Gastrosvět.cz

OpenAIRE

Procházka, Petr

2010-01-01

The aim of this thesis is a description of the planning, establishment and development of advertising portal Gastrosvět.cz, as the actual project conducted by the Business Incubator of VŠE. The thesis is divided into theoretical and practical parts. The theoretical part explains and descibes used terms like business, businessman, SWOT analysis, balance sheet, profit and loss account, cash flow, break-point analysis, legal forms of business enterprise, life cycle of a business plan, financing ...

Equine Science. Instructor Guide [and] Student Reference. Volume 27, Number 4 [and] Volume 27, Number 5.

Science.gov (United States)

Leffert, Kenneth L.; And Others

This instructor guide and the corresponding student reference contain 10 lessons to enhance an Agricultural Science I course for grade 9. The lessons cover the following topics: introduction, psychology and handling, conformation and selection, genetics and reproduction, herd health, hoof care, nutrition, equipment and facilities, handling horses,…

[Genetically modified food--unnecessary controversy?].

Science.gov (United States)

Tchórz, Michał; Radoniewicz-Chagowska, Anna; Lewandowska-Stanek, Hanna; Szponar, Elzbieta; Szponar, Jarosław

2012-01-01

Fast development of genetic engineering and biotechnology allows use of genetically modified organisms (GMO) more and more in different branches of science and economy. Every year we can see an increase of food amount produced with the use of modification of genetic material. In our supermarkets we can find brand new types of plants, products including genetically modified ingredients or meat from animals fed with food containing GMO. This article presents general information about genetically modified organisms, it also explains the range of genetic manipulation, use of newly developed products and current field area for GMO in the world. Based on scientific data the article presents benefits from development of biotechnology in reference to modified food. It also presents the voice of skeptics who are extremely concerned about the impact of those organisms on human health and natural environment. Problems that appear or can appear as a result of an increase of GMO are very important not only from a toxicologist's or a doctor's point of view but first of all from the point of view of ordinary consumers--all of us.

Tvorba vs. štěpení C-C vazeb katalyzované komplexy niklu

Czech Academy of Sciences Publication Activity Database

Nečas, D.; Turský, M.; Kotora, Martin

2005-01-01

Roč. 99, č. 11 (2005), s. 861 ISSN 0009-2770. [Pokroky v organické, bioorganické a farmaceutické chemii /40./. 18.11.2005-20.11.2005, Nymburk] R&D Projects: GA MŠk(CZ) 1M0508; GA ČR(CZ) GD203/03/H140 Institutional research plan: CEZ:AV0Z40550506 Keywords : bipyridine * cyclotrimerization * asymmetric synthesis Subject RIV: CC - Organic Chemistry

Recruitment of mitochondrial uncoupling protein UCP2 after lipopolysaccharide induction

Czech Academy of Sciences Publication Activity Database

Růžička, Michal; Škobisová, Eva; Dlasková, Andrea; Šantorová, Jitka; Smolková, Katarína; Špaček, Tomáš; Žáčková, Markéta; Modrianský, M.; Ježek, Petr

2005-01-01

Roč. 37, č. 4 (2005), s. 809-821 ISSN 1357-2725 R&D Projects: GA ČR(CZ) GA301/02/1215; GA ČR(CZ) GP301/01/P084; GA AV ČR(CZ) IAA5011106 Grant - others:NIH(US) TW01487 Institutional research plan: CEZ:AV0Z5011922 Keywords : lipopolysaccharide * oxidative stress in liver * mitochondrial uncoupling protein UCP2 Subject RIV: CE - Biochemistry Impact factor: 3.871, year: 2005

Thin nanodiamond membranes and their microstructural, optical and photoelectrical properties

Czech Academy of Sciences Publication Activity Database

Mortet, V.; D´Haen, J.; Potměšil, Jiří; Kravets, Roman; Drbohlav, Ivo; Vorlíček, Vladimír; Rosa, Jan; Vaněček, Milan

2005-01-01

Roč. 14, - (2005), s. 393-397 ISSN 0925-9635 R&D Projects: GA MŠk(CZ) LN00A015; GA ČR(CZ) GA202/05/2233; GA MŠk(CZ) LC510 EU Projects: European Commission(XE) HPRN-CT-1999-00139 Institutional research plan: CEZ:AV0Z10100521 Keywords : nanodiamond * structural characterization * optical properties * defect spectroscopy Subject RIV: BM - Solid Matter Physics ; Magnetism Impact factor: 1.988, year: 2005

Carboranethiol-modified gold surfaces. A study and comparison of modified cluster and flat surfaces

Czech Academy of Sciences Publication Activity Database

Baše, Tomáš; Bastl, Zdeněk; Plzák, Zbyněk; Grygar, Tomáš; Plešek, Jaromír; Carr, MJ.; Malina, Václav; Šubrt, Jan; Boháček, Jaroslav; Večerníková, Eva; Kříž, Otomar

2005-01-01

Roč. 21, č. 17 (2005), s. 7776-7785 ISSN 0743-7463 R&D Projects: GA AV ČR(CZ) KJB4032302; GA AV ČR(CZ) KSK4040110; GA AV ČR(CZ) 1ET400400413 Grant - others:EPSRC(GB) L/49505; EPSRC(GB) R/61949 Institutional research plan: CEZ:AV0Z40320502 Keywords : SELF-ASSEMBLED MONOLAYERS * ray photoelectron-spectroscopy * strucrural characterization Subject RIV: CA - Inorganic Chemistry Impact factor: 3.705, year: 2005