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Sample records for allele-specific patterns adopted

  1. Novel method for analysis of allele specific expression in triploid Oryzias latipes reveals consistent pattern of allele exclusion.

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    Tzintzuni I Garcia

    Full Text Available Assessing allele-specific gene expression (ASE on a large scale continues to be a technically challenging problem. Certain biological phenomena, such as X chromosome inactivation and parental imprinting, affect ASE most drastically by completely shutting down the expression of a whole set of alleles. Other more subtle effects on ASE are likely to be much more complex and dependent on the genetic environment and are perhaps more important to understand since they may be responsible for a significant amount of biological diversity. Tools to assess ASE in a diploid biological system are becoming more reliable. Non-diploid systems are, however, not uncommon. In humans full or partial polyploid states are regularly found in both healthy (meiotic cells, polynucleated cell types and diseased tissues (trisomies, non-disjunction events, cancerous tissues. In this work we have studied ASE in the medaka fish model system. We have developed a method for determining ASE in polyploid organisms from RNAseq data and we have implemented this method in a software tool set. As a biological model system we have used nuclear transplantation to experimentally produce artificial triploid medaka composed of three different haplomes. We measured ASE in RNA isolated from the livers of two adult, triploid medaka fish that showed a high degree of similarity. The majority of genes examined (82% shared expression more or less evenly among the three alleles in both triploids. The rest of the genes (18% displayed a wide range of ASE levels. Interestingly the majority of genes (78% displayed generally consistent ASE levels in both triploid individuals. A large contingent of these genes had the same allele entirely suppressed in both triploids. When viewed in a chromosomal context, it is revealed that these genes are from large sections of 4 chromosomes and may be indicative of some broad scale suppression of gene expression.

  2. Allele-specific gene expression patterns in primary leukemic cells reveal regulation of gene expression by CpG site methylation

    DEFF Research Database (Denmark)

    Milani, Lili; Lundmark, Anders; Nordlund, Jessica;

    2008-01-01

    To identify genes that are regulated by cis-acting functional elements in acute lymphoblastic leukemia (ALL) we determined the allele-specific expression (ASE) levels of 2, 529 genes by genotyping a genome-wide panel of single nucleotide polymorphisms in RNA and DNA from bone marrow and blood sam...... of these sites. Our results demonstrate that CpG site methylation is one of the factors that regulates gene expression in ALL cells....

  3. Ontology Design Patterns: Adoption Challenges and Solutions

    OpenAIRE

    Hammar, Karl

    2014-01-01

    Ontology Design Patterns (ODPs) are intended to guide non-experts in performing ontology engineering tasks successfully. While being the topic of significant research efforts, the uptake of these ideas outside the academic community is limited. This paper summarises some issues preventing broader adoption of Ontology Design Patterns among practitioners, suggests research directions that may help overcome these issues, and presents early results of work in these directions.

  4. Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus

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    Fujita, Toshitsugu; Yuno, Miyuki; Fujii, Hodaka

    2016-01-01

    The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adopted for a wide range of biological applications including genome editing. In some cases, dissection of genome functions requires allele-specific genome editing, but the use of CRISPR for this purpose has not been studied in detail. In this study, using the p16INK4a gene in HCT116 as a model locus, we investigated whether chromatin states, such as CpG methylation, or a single-nucleotide gap form in a target site can be exploited for allele-specific locus binding and genome editing by CRISPR in vivo. First, we showed that allele-specific locus binding and genome editing could be achieved by targeting allele-specific CpG-methylated regions, which was successful for one, but not all guide RNAs. In this regard, molecular basis underlying the success remains elusive at this stage. Next, we demonstrated that an allele-specific single-nucleotide gap form could be employed for allele-specific locus binding and genome editing by CRISPR, although it was important to avoid CRISPR tolerance of a single nucleotide mismatch brought about by mismatched base skipping. Our results provide information that might be useful for applications of CRISPR in studies of allele-specific functions in the genomes. PMID:27465215

  5. Adopting Collaborative Workflow Pattern : Use Case

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    Antonio Capodieci

    2015-03-01

    Full Text Available In recent years, the use of web 2.0 tools has incre ased in companies and organisation. This phenomenon, has modified common organisational and operative practices. This has led “knowledge workers” to change their working practic es through the use of Web 2.0 communication tools. Unfortunately, these tools hav e not been integrated with existing enterprise information systems. This is an importan t problem in an organisational context because knowledge of information exchanged is neede d. In previous works we demonstrate that it is possible to capture this knowledge using coll aboration processes, which are processes of abstraction created in accordance with design patte rns and applied to new organisational operative practices. In this article, we want to pr esent the experience of the adoption of the methodology and the catalog of patterns from some p attern designers of a company operating in Public Administration and Finance, with the aim of shaping an information system Enterprise 2.0, which takes into account the collaborative pro cesses.

  6. Allele-Specific DNA Methylation Detection by Pyrosequencing®

    DEFF Research Database (Denmark)

    Sommer Kristensen, Lasse; Johansen, Jens Vilstrup; Grønbæk, Kirsten

    2015-01-01

    DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide......-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing(®) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon....

  7. Typing for HLA-DPB1*03 and HLA-DPB1*06 using allele-specific DNA in vitro amplification and allele-specific oligonucleotide probes. Detection of "new" DPB1*06 variants

    DEFF Research Database (Denmark)

    Fugger, L; Morling, N; Ryder, L P;

    1989-01-01

    DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes has recently been reported. The resulting DNA amplification was specific for the HLA-DPB locus. Typing for the individual DPB alleles was exclusively dependent on the hybridizations of the probes...... but hampered by close sequence homology between different DP alleles yielding complex patterns of reactivity with a panel of probes. We report the combined use of allele-specific DNA in vitro amplification and allele-specific oligonucleotides in typing for DPB1*03 and DPB1*06. Complete concordance with PLT...

  8. Understanding Community Policing as an Innovation: Patterns of Adoption

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    Morabito, Melissa Schaefer

    2010-01-01

    In the 1980s and 1990s, community policing was viewed by many as a radical innovation in the field of policing, with the vast majority of police agencies reporting to have adopted the approach. Despite its overwhelming popularity, most police agencies did not adopt the central elements of community policing. This study examines patterns of…

  9. Disease-Causing Allele-Specific Silencing by RNA Interference

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    Hirohiko Hohjoh

    2013-04-01

    Full Text Available Small double-stranded RNAs (dsRNAs of approximately 21-nucleotides in size, referred to as small interfering RNA (siRNA duplexes, can induce sequence-specific posttranscriptional gene silencing, or RNA interference (RNAi. Since chemically synthesized siRNA duplexes were found to induce RNAi in mammalian cells, RNAi has become a powerful reverse genetic tool for suppressing the expression of a gene of interest in mammals, including human, and its application has been expanding to various fields. Recent studies further suggest that synthetic siRNA duplexes have the potential for specifically inhibiting the expression of an allele of interest without suppressing the expression of other alleles, i.e., siRNA duplexes likely confer allele-specific silencing. Such gene silencing by RNAi is an advanced technique with very promising applications. In this review, I would like to discuss the potential utility of allele-specific silencing by RNAi as a therapeutic method for dominantly inherited diseases, and describe possible improvements in siRNA duplexes for enhancing their efficacy.

  10. Allele-specific DNA methylation reinforces PEAR1 enhancer activity.

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    Izzi, Benedetta; Pistoni, Mariaelena; Cludts, Katrien; Akkor, Pinar; Lambrechts, Diether; Verfaillie, Catherine; Verhamme, Peter; Freson, Kathleen; Hoylaerts, Marc F

    2016-08-18

    Genetic variation in the PEAR1 locus is linked to platelet reactivity and cardiovascular disease. The major G allele of rs12041331, an intronic cytosine guanine dinucleotide-single-nucleotide polymorphism (CpG-SNP), is associated with higher PEAR1 expression in platelets and endothelial cells than the minor A allele. The molecular mechanism underlying this difference remains elusive. We have characterized the histone modification profiles of the intronic region surrounding rs12041331 and identified H3K4Me1 enhancer-specific enrichment for the region that covers the CpG-SNP. Interestingly, methylation studies revealed that the CpG site is fully methylated in leukocytes of GG carriers. Nuclear protein extracts from megakaryocytes, endothelial cells, vs control HEK-293 cells show a 3-fold higher affinity for the methylated G allele compared with nonmethylated G or A alleles in a gel electrophoretic mobility shift assay. To understand the positive relationship between methylation and gene expression, we studied DNA methylation at 4 different loci of PEAR1 during in vitro megakaryopoiesis. During differentiation, the CpG-SNP remained fully methylated, while we observed rapid methylation increases at the CpG-island overlapping the first 5'-untranslated region exon, paralleling the increased PEAR1 expression. In the same region, A-allele carriers of rs12041331 showed significantly lower DNA methylation at CGI1 compared with GG homozygote. This CpG-island contains binding sites for the methylation-sensitive transcription factor CTCF, whose binding is known to play a role in enhancer activation and/or repression. In conclusion, we report the molecular characterization of the first platelet function-related CpG-SNP, a genetic predisposition that reinforces PEAR1 enhancer activity through allele-specific DNA methylation. PMID:27313330

  11. Allele-specific analysis of DNA replication origins in mammalian cells.

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    Bartholdy, Boris; Mukhopadhyay, Rituparna; Lajugie, Julien; Aladjem, Mirit I; Bouhassira, Eric E

    2015-01-01

    The mechanisms that control the location and timing of firing of replication origins are poorly understood. Using a novel functional genomic approach based on the analysis of SNPs and indels in phased human genomes, we observe that replication asynchrony is associated with small cumulative variations in the initiation efficiency of multiple origins between the chromosome homologues, rather than with the activation of dormant origins. Allele-specific measurements demonstrate that the presence of G-quadruplex-forming sequences does not correlate with the efficiency of initiation. Sequence analysis reveals that the origins are highly enriched in sequences with profoundly asymmetric G/C and A/T nucleotide distributions and are almost completely depleted of antiparallel triplex-forming sequences. We therefore propose that although G4-forming sequences are abundant in replication origins, an asymmetry in nucleotide distribution, which increases the propensity of origins to unwind and adopt non-B DNA structure, rather than the ability to form G4, is directly associated with origin activity. PMID:25987481

  12. Allele-specific deposition of macroH2A1 in Imprinting Control Regions

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    Choo, J H; Kim, J D; Chung, J H; Stubbs, L; Kim, J

    2006-01-13

    In the current study, we analyzed the deposition patterns of macroH2A1 at a number of different genomic loci located in X chromosome and autosomes. MacroH2A1 is preferentially deposited at methylated CpG CpG-rich regions located close to promoters. The macroH2A1 deposition patterns at the methylated CpG islands of several imprinted domains, including the Imprinting Control Regions (ICRs) of Xist, Peg3, H19/Igf2 Igf2, Gtl2/Dlk1, and Gnas domains, show consistent allele-specificity towards inactive, methylated alleles. The macroH2A1 deposition levels at the ICRs and other Differentially Methylated Regions (DMRs) of these domains are also either higher or comparable to those observed at the inactive X chromosome of female mammals. Overall, our results indicate that besides DNA methylation macroH2A1 is another epigenetic component in the chromatin of ICRs displaying differential association with two parental alleles.

  13. H19-DMR allele-specific methylation analysis reveals epigenetic heterogeneity of CTCF binding site 6 but not of site 5 in head-and-neck carcinomas

    DEFF Research Database (Denmark)

    De Castro Valente Esteves, Leda Isabel; De Karla Cervigne, Nilva; Do Carmo Javaroni, Afonso;

    2006-01-01

    Aberrant methylation of seven potential binding sites of the CTCF factor in the differentially methylated region upstream of the H19 gene (H19-DMR) has been suggested as critical for the regulation of IGF2 and H19 imprinted genes. In this study, we analyzed the allele-specific methylation pattern...

  14. Allele-specific methylation occurs at genetic variants associated with complex disease.

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    John N Hutchinson

    Full Text Available We hypothesize that the phenomenon of allele-specific methylation (ASM may underlie the phenotypic effects of multiple variants identified by Genome-Wide Association studies (GWAS. We evaluate ASM in a human population and document its genome-wide patterns in an initial screen at up to 380,678 sites within the genome, or up to 5% of the total genomic CpGs. We show that while substantial inter-individual variation exists, 5% of assessed sites show evidence of ASM in at least six samples; the majority of these events (81% are under genetic influence. Many of these cis-regulated ASM variants are also eQTLs in peripheral blood mononuclear cells and monocytes and/or in high linkage-disequilibrium with variants linked to complex disease. Finally, focusing on autoimmune phenotypes, we extend this initial screen to confirm the association of cis-regulated ASM with multiple complex disease-associated variants in an independent population using next-generation bisulfite sequencing. These four variants are implicated in complex phenotypes such as ulcerative colitis and AIDS progression disease (rs10491434, Celiac disease (rs2762051, Crohn's disease, IgA nephropathy and early-onset inflammatory bowel disease (rs713875 and height (rs6569648. Our results suggest cis-regulated ASM may provide a mechanistic link between the non-coding genetic changes and phenotypic variation observed in these diseases and further suggests a route to integrating DNA methylation status with GWAS results.

  15. Assessing allele-specific expression across multiple tissues from RNA-seq read data

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    Pirinen, Matti; Lappalainen, Tuuli; Zaitlen, Noah A.; Dermitzakis, Emmanouil T.; Donnelly, Peter; McCarthy, Mark I.; Rivas, Manuel A.

    2015-01-01

    Motivation: RNA sequencing enables allele-specific expression (ASE) studies that complement standard genotype expression studies for common variants and, importantly, also allow measuring the regulatory impact of rare variants. The Genotype-Tissue Expression (GTEx) project is collecting RNA-seq data on multiple tissues of a same set of individuals and novel methods are required for the analysis of these data. Results: We present a statistical method to compare different patterns of ASE across tissues and to classify genetic variants according to their impact on the tissue-wide expression profile. We focus on strong ASE effects that we are expecting to see for protein-truncating variants, but our method can also be adjusted for other types of ASE effects. We illustrate the method with a real data example on a tissue-wide expression profile of a variant causal for lipoid proteinosis, and with a simulation study to assess our method more generally. Availability and implementation: http://www.well.ox.ac.uk/~rivas/mamba/. R-sources and data examples http://www.iki.fi/mpirinen/ Contact: matti.pirinen@helsinki.fi or rivas@well.ox.ac.uk Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25819081

  16. Allele Workbench: transcriptome pipeline and interactive graphics for allele-specific expression.

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    Carol A Soderlund

    Full Text Available Sequencing the transcriptome can answer various questions such as determining the transcripts expressed in a given species for a specific tissue or condition, evaluating differential expression, discovering variants, and evaluating allele-specific expression. Differential expression evaluates the expression differences between different strains, tissues, and conditions. Allele-specific expression evaluates expression differences between parental alleles. Both differential expression and allele-specific expression have been studied for heterosis (hybrid vigor, where the hybrid has improved performance over the parents for one or more traits. The Allele Workbench software was developed for a heterosis study that evaluated allele-specific expression for a mouse F1 hybrid using libraries from multiple tissues with biological replicates. This software has been made into a distributable package, which includes a pipeline, a Java interface to build the database, and a Java interface for query and display of the results. The required input is a reference genome, annotation file, and one or more RNA-Seq libraries with optional replicates. It evaluates allelic imbalance at the SNP and transcript level and flags transcripts with significant opposite directional allele-specific expression. The Java interface allows the user to view data from libraries, replicates, genes, transcripts, exons, and variants, including queries on allele imbalance for selected libraries. To determine the impact of allele-specific SNPs on protein folding, variants are annotated with their effect (e.g., missense, and the parental protein sequences may be exported for protein folding analysis. The Allele Workbench processing results in transcript files and read counts that can be used as input to the previously published Transcriptome Computational Workbench, which has a new algorithm for determining a trimmed set of gene ontology terms. The software with demo files is available

  17. Epidemiological survey of Theileria parasite infection of cattle in Northeast China by allele-specific PCR.

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    Yu, Longzheng; Zhang, Shoufa; Liang, Wanfeng; Jin, Chunmei; Jia, Lijun; Luo, Yuzi; Li, Yan; Cao, Shinuo; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawano, Suguru; Fujisaki, Kozo; Xuan, Xuenan

    2011-11-01

    An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.

  18. Internet adoption and usage patterns in Africa: Evidence from Cameroon

    OpenAIRE

    Penard, Thierry; Poussing, Nicolas; MUKOKO Blaise; TAMOKWE Georges Bertrand

    2013-01-01

    The objective of this paper is to understand what factors stimulate or hinder the adoption and usage of the Internet in Africa. We adopt a micro-econometric approach and use household survey data from Cameroon. Our results show that Internet users in Cameroon tend to be young, educated and in employment. The probability of using the Internet is also higher for male, as well as for English-speaking and computer savvy individuals. Moreover, Internet users are more likely to have family abroad. ...

  19. Allele-specific copy number profiling by next-generation DNA sequencing.

    Science.gov (United States)

    Chen, Hao; Bell, John M; Zavala, Nicolas A; Ji, Hanlee P; Zhang, Nancy R

    2015-02-27

    The progression and clonal development of tumors often involve amplifications and deletions of genomic DNA. Estimation of allele-specific copy number, which quantifies the number of copies of each allele at each variant loci rather than the total number of chromosome copies, is an important step in the characterization of tumor genomes and the inference of their clonal history. We describe a new method, falcon, for finding somatic allele-specific copy number changes by next generation sequencing of tumors with matched normals. falcon is based on a change-point model on a bivariate mixed Binomial process, which explicitly models the copy numbers of the two chromosome haplotypes and corrects for local allele-specific coverage biases. By using the Binomial distribution rather than a normal approximation, falcon more effectively pools evidence from sites with low coverage. A modified Bayesian information criterion is used to guide model selection for determining the number of copy number events. Falcon is evaluated on in silico spike-in data and applied to the analysis of a pre-malignant colon tumor sample and late-stage colorectal adenocarcinoma from the same individual. The allele-specific copy number estimates obtained by falcon allows us to draw detailed conclusions regarding the clonal history of the individual's colon cancer. PMID:25477383

  20. Allele-specific amplification and electrochemiluminescence method for single nucleotide polymorphism analysis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A new approach combined the specificity of allele-specific amplification (ASA) with the sensitivity of electrochemiluminescence (ECL) assay for single nucleotide polymorphism (SNP) analysis was proposed. Briefly, target gene was amplified by a biotin-labeled allele-specific forward primer and a Ru(bpy)32+ (TBR)-labeled universal reverse primer. Then, the amplicon was captured onto streptavidin-coated paramagnetic beads through biotin label, and detected by measuring the ECL signal of TBR label. Different genotypes were distinguished according to the ECL values of the amplicons by different genotypic primers. K-ras oncogene was used as a target to validate the feasibility of the method. The experiment results show that the different genotypes can be clearly distinguished by ASA-ECL assay. The method is useful in SNP analysis due to its sensitivity,safety, and simplicity.(C) 2007 Da Xing. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

  1. Detection of mutation by allele-specific loop-mediated isothermal amplification (AS-LAMP).

    Science.gov (United States)

    Aonuma, Hiroka; Badolo, Athanase; Okado, Kiyoshi; Kanuka, Hirotaka

    2013-01-01

    For effective control of pathogen-transmitting mosquitoes, precise surveillance data of mosquito distribution are essential. Recently, an increase of insecticide resistance due to the kdr mutation in Anopheles gambiae, a mosquito that transmits the malaria parasite, has been reported. With the aim of developing a simple and effective method for surveying resistant mosquitoes, LAMP was applied to the allele-specific detection of the kdr gene in An. gambiae. Allele-specific LAMP (AS-LAMP) method successfully distinguished the kdr homozygote from the heterozygote and the wild type. The robustness of AS-LAMP suggests its usefulness for routine identification of insects, not only mosquitoes but also other vectors and agricultural pests. Here we describe the method of AS-LAMP to detect mutation in Anopheles mosquitoes. PMID:24026691

  2. Determination of DQB1 alleles using PCR amplification and allele-specific primers.

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    Lepage, V; Ivanova, R; Loste, M N; Mallet, C; Douay, C; Naoumova, E; Charron, D

    1995-10-01

    Molecular genotyping of HLA class II genes is commonly carried out using polymerase chain reaction (PCR) in combination with sequence-specific oligotyping (PCR-SSO) or a combination of the PCR and restriction fragment length polymorphism methods (PCR-RFLP). However, the identification of the DQB1 type by PCR-SSO and PCR-RFLP is very time-consuming which is disadvantageous for the typing of cadaveric organ donors. We have developed a DQB1 typing method using PCR in combination with allele-specific amplification (PCR-ASA), which allows the identification of the 17 most frequent alleles in one step using seven amplification mixtures. PCR allele-specific amplification HLA-DQB1 typing is easy to perform, and the results are easy to interpret in routine clinical practice. The PCR-ASA method is therefore better suited to DQB1 typing for organ transplantation than other methods.

  3. An improved allele-specific PCR primer design method for SNP marker analysis and its application

    OpenAIRE

    Liu Jing; Huang Shunmou; Sun Meiyu; Liu Shengyi; Liu Yumei; Wang Wanxing; Zhang Xiurong; Wang Hanzhong; Hua Wei

    2012-01-01

    Abstract Background Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) still restricts its application as molecular marker like other markers such as Simple Sequence Repeat (SSR). To overcome this problem, primers with a single nucleotide artificial mismatch introduced within the three bases closest to the 3’end (SNP site) have been used in ...

  4. High-speed droplet-allele-specific polymerase chain reaction for genotyping of single nucleotide polymorphisms.

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    Matsuda, Kazuyuki; Honda, Takayuki

    2015-01-01

    Single nucleotide alternations such as single nucleotide polymorphisms (SNPs) or single nucleotide mutations are useful genetic markers for molecular diagnosis, prognosis, drug response, and predisposition to diseases. Rapid identification of SNPs or mutations is clinically important, especially for determining drug responses and selection of molecular-targeted therapy. Here, we describe a rapid genotyping assay based on the allele-specific polymerase chain reaction (AS-PCR) by using our droplet-PCR machine (droplet-AS-PCR).

  5. Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping.

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    Lee, Han B; Schwab, Tanya L; Koleilat, Alaa; Ata, Hirotaka; Daby, Camden L; Cervera, Roberto Lopez; McNulty, Melissa S; Bostwick, Hannah S; Clark, Karl J

    2016-06-01

    Customizable endonucleases such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) enable rapid generation of mutant strains at genomic loci of interest in animal models and cell lines. With the accelerated pace of generating mutant alleles, genotyping has become a rate-limiting step to understanding the effects of genetic perturbation. Unless mutated alleles result in distinct morphological phenotypes, mutant strains need to be genotyped using standard methods in molecular biology. Classic restriction fragment length polymorphism (RFLP) or sequencing is labor-intensive and expensive. Although simpler than RFLP, current versions of allele-specific PCR may still require post-polymerase chain reaction (PCR) handling such as sequencing, or they are more expensive if allele-specific fluorescent probes are used. Commercial genotyping solutions can take weeks from assay design to result, and are often more expensive than assembling reactions in-house. Key components of commercial assay systems are often proprietary, which limits further customization. Therefore, we developed a one-step open-source genotyping method based on quantitative PCR. The allele-specific qPCR (ASQ) does not require post-PCR processing and can genotype germline mutants through either threshold cycle (Ct) or end-point fluorescence reading. ASQ utilizes allele-specific primers, a locus-specific reverse primer, universal fluorescent probes and quenchers, and hot start DNA polymerase. Individual laboratories can further optimize this open-source system as we completely disclose the sequences, reagents, and thermal cycling protocol. We have tested the ASQ protocol to genotype alleles in five different genes. ASQ showed a 98-100% concordance in genotype scoring with RFLP or Sanger sequencing outcomes. ASQ is time-saving because a single qPCR without post-PCR handling suffices to score

  6. SNPsplit: Allele-specific splitting of alignments between genomes with known SNP genotypes.

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    Krueger, Felix; Andrews, Simon R

    2016-01-01

    Sequencing reads overlapping polymorphic sites in diploid mammalian genomes may be assigned to one allele or the other. This holds the potential to detect gene expression, chromatin modifications, DNA methylation or nuclear interactions in an allele-specific fashion. SNPsplit is an allele-specific alignment sorter designed to read files in SAM/BAM format and determine the allelic origin of reads or read-pairs that cover known single nucleotide polymorphic (SNP) positions. For this to work libraries must have been aligned to a genome in which all known SNP positions were masked with the ambiguity base 'N' and aligned using a suitable mapping program such as Bowtie2, TopHat, STAR, HISAT2, HiCUP or Bismark. SNPsplit also provides an automated solution to generate N-masked reference genomes for hybrid mouse strains based on the variant call information provided by the Mouse Genomes Project. The unique ability of SNPsplit to work with various different kinds of sequencing data including RNA-Seq, ChIP-Seq, Bisulfite-Seq or Hi-C opens new avenues for the integrative exploration of allele-specific data. PMID:27429743

  7. Adoption Patterns for the Digital Post System by Danish Municipalities and Citizens

    DEFF Research Database (Denmark)

    Berger, Jesper B.; Hertzum, Morten

    2014-01-01

    The value of e-government, services to citizens by public institutions through the internet, is dependent on the mutual adoption of e-government by both the public institution and the citizens. This paper describes a longitudinal study of e-government adoption by municipalities and citizens in...... Denmark. We studied the e-government initiative Digital Post ¬¬– encrypted digital communication between municipalities and citizens. Over the three-year adoption period, we found four adoption patterns among municipalities, characterized by a slow, late, gradual or early increase in the use of Digital...... Post. The adoption patterns among citizens were less distinct. We calculated the realized savings to only 20% of the anticipated savings, leading to a deficit of nearly €5 million in 2013. Municipal funding was reduced according to the anticipated savings. In addition, the variation in adoption by...

  8. Adoption Patterns for the Digital Post System by Danish Municipalities and Citizens

    DEFF Research Database (Denmark)

    Berger, Jesper Bull; Hertzum, Morten

    2014-01-01

    . The adoption patterns among citizens were less distinct. We calculated the realized savings to only 20% of the anticipated savings, leading to a deficit of nearly €5 million in 2013. Municipal funding was reduced according to the anticipated savings. In addition, the variation in adoption by municipal staff...

  9. A genome-wide screen in human embryonic stem cells reveals novel sites of allele-specific histone modification associated with known disease loci

    LENUS (Irish Health Repository)

    Prendergast, James G D

    2012-05-19

    AbstractBackgroundChromatin structure at a given site can differ between chromosome copies in a cell, and such imbalances in chromatin structure have been shown to be important in understanding the molecular mechanisms controlling several disease loci. Human genetic variation, DNA methylation, and disease have been intensely studied, uncovering many sites of allele-specific DNA methylation (ASM). However, little is known about the genome-wide occurrence of sites of allele-specific histone modification (ASHM) and their relationship to human disease. The aim of this study was to investigate the extent and characteristics of sites of ASHM in human embryonic stem cells (hESCs).ResultsUsing a statistically rigorous protocol, we investigated the genomic distribution of ASHM in hESCs, and their relationship to sites of allele-specific expression (ASE) and DNA methylation. We found that, although they were rare, sites of ASHM were substantially enriched at loci displaying ASE. Many were also found at known imprinted regions, hence sites of ASHM are likely to be better markers of imprinted regions than sites of ASM. We also found that sites of ASHM and ASE in hESCs colocalize at risk loci for developmental syndromes mediated by deletions, providing insights into the etiology of these disorders.ConclusionThese results demonstrate the potential importance of ASHM patterns in the interpretation of disease loci, and the protocol described provides a basis for similar studies of ASHM in other cell types to further our understanding of human disease susceptibility.

  10. Allele-specific gene silencing in two mouse models of autosomal dominant skeletal myopathy.

    Directory of Open Access Journals (Sweden)

    Ryan E Loy

    Full Text Available We explored the potential of mutant allele-specific gene silencing (ASGS in providing therapeutic benefit in two established mouse models of the autosomal dominantly-inherited muscle disorders, Malignant Hyperthermia (MH and Central Core Disease (CCD. Candidate ASGS siRNAs were designed and validated for efficacy and specificity on ryanodine receptor (RyR1 cDNA mini-constructs expressed in HEK293 cells using RT-PCR- and confocal microscopy-based assays. In vivo delivery of the most efficacious identified siRNAs into flexor digitorum brevis (FDB muscles was achieved by injection/electroporation of footpads of 4-6 month old heterozygous Ryr1(Y524S/+ (YS/+ and Ryr1(I4895T/+ (IT/+ knock-in mice, established mouse models of MH with cores and CCD, respectively. Treatment of IT/+ mice resulted in a modest rescue of deficits in the maximum rate (∼38% rescue and magnitude (∼78% of ligand-induced Ca(2+ release that occurred in the absence of a change in the magnitude of electrically-evoked Ca(2+ release. Compared to the difference between the caffeine sensitivity of Ca(2+ release in FDB fibers from YS/+ and WT mice treated with SCR siRNA (EC(50: 1.1 mM versus 4.4 mM, respectively, caffeine sensitivity was normalized in FDB fibers from YS/+ mice following 2 (EC(50: 2.8 mM and 4 week (EC(50: 6.6 mM treatment with YS allele-specific siRNA. Moreover, the temperature-dependent increase in resting Ca(2+ observed in FDB fibers from YS/+ mice was normalized to WT levels after 2 weeks of treatment with YS allele-specific siRNA. As determined by quantitative real time PCR, the degree of functional rescue in YS/+ and IT/+ mice correlated well with the relative increase in fractional WT allele expression.

  11. Allele-specific silencing of mutant Ataxin-7 in SCA7 patient-derived fibroblasts.

    Science.gov (United States)

    Scholefield, Janine; Watson, Lauren; Smith, Danielle; Greenberg, Jacquie; Wood, Matthew J A

    2014-12-01

    Polyglutamine (polyQ) disorders are inherited neurodegenerative conditions defined by a common pathogenic CAG repeat expansion leading to a toxic gain-of-function of the mutant protein. Consequences of this toxicity include activation of heat-shock proteins (HSPs), impairment of the ubiquitin-proteasome pathway and transcriptional dysregulation. Several studies in animal models have shown that reducing levels of toxic protein using small RNAs would be an ideal therapeutic approach for such disorders, including spinocerebellar ataxia-7 (SCA7). However, testing such RNA interference (RNAi) effectors in genetically appropriate patient cell lines with a disease-relevant phenotype has yet to be explored. Here, we have used primary adult dermal fibroblasts from SCA7 patients and controls to assess the endogenous allele-specific silencing of ataxin-7 by two distinct siRNAs. We further identified altered expression of two disease-relevant transcripts in SCA7 patient cells: a twofold increase in levels of the HSP DNAJA1 and a twofold decrease in levels of the de-ubiquitinating enzyme, UCHL1. After siRNA treatment, the expression of both genes was restored towards normal levels. To our knowledge, this is the first time that allele-specific silencing of mutant ataxin-7, targeting a common SNP, has been demonstrated in patient cells. These findings highlight the advantage of an allele-specific RNAi-based therapeutic approach, and indicate the value of primary patient-derived cells as useful models for mechanistic studies and for measuring efficacy of RNAi effectors on a patient-to-patient basis in the polyQ diseases. PMID:24667781

  12. Allele-specific amplification in cancer revealed by SNP array analysis.

    Directory of Open Access Journals (Sweden)

    Thomas LaFramboise

    2005-11-01

    Full Text Available Amplification, deletion, and loss of heterozygosity of genomic DNA are hallmarks of cancer. In recent years a variety of studies have emerged measuring total chromosomal copy number at increasingly high resolution. Similarly, loss-of-heterozygosity events have been finely mapped using high-throughput genotyping technologies. We have developed a probe-level allele-specific quantitation procedure that extracts both copy number and allelotype information from single nucleotide polymorphism (SNP array data to arrive at allele-specific copy number across the genome. Our approach applies an expectation-maximization algorithm to a model derived from a novel classification of SNP array probes. This method is the first to our knowledge that is able to (a determine the generalized genotype of aberrant samples at each SNP site (e.g., CCCCT at an amplified site, and (b infer the copy number of each parental chromosome across the genome. With this method, we are able to determine not just where amplifications and deletions occur, but also the haplotype of the region being amplified or deleted. The merit of our model and general approach is demonstrated by very precise genotyping of normal samples, and our allele-specific copy number inferences are validated using PCR experiments. Applying our method to a collection of lung cancer samples, we are able to conclude that amplification is essentially monoallelic, as would be expected under the mechanisms currently believed responsible for gene amplification. This suggests that a specific parental chromosome may be targeted for amplification, whether because of germ line or somatic variation. An R software package containing the methods described in this paper is freely available at http://genome.dfci.harvard.edu/~tlaframb/PLASQ.

  13. Allele-specific silencing of mutant Ataxin-7 in SCA7 patient-derived fibroblasts.

    Science.gov (United States)

    Scholefield, Janine; Watson, Lauren; Smith, Danielle; Greenberg, Jacquie; Wood, Matthew J A

    2014-12-01

    Polyglutamine (polyQ) disorders are inherited neurodegenerative conditions defined by a common pathogenic CAG repeat expansion leading to a toxic gain-of-function of the mutant protein. Consequences of this toxicity include activation of heat-shock proteins (HSPs), impairment of the ubiquitin-proteasome pathway and transcriptional dysregulation. Several studies in animal models have shown that reducing levels of toxic protein using small RNAs would be an ideal therapeutic approach for such disorders, including spinocerebellar ataxia-7 (SCA7). However, testing such RNA interference (RNAi) effectors in genetically appropriate patient cell lines with a disease-relevant phenotype has yet to be explored. Here, we have used primary adult dermal fibroblasts from SCA7 patients and controls to assess the endogenous allele-specific silencing of ataxin-7 by two distinct siRNAs. We further identified altered expression of two disease-relevant transcripts in SCA7 patient cells: a twofold increase in levels of the HSP DNAJA1 and a twofold decrease in levels of the de-ubiquitinating enzyme, UCHL1. After siRNA treatment, the expression of both genes was restored towards normal levels. To our knowledge, this is the first time that allele-specific silencing of mutant ataxin-7, targeting a common SNP, has been demonstrated in patient cells. These findings highlight the advantage of an allele-specific RNAi-based therapeutic approach, and indicate the value of primary patient-derived cells as useful models for mechanistic studies and for measuring efficacy of RNAi effectors on a patient-to-patient basis in the polyQ diseases.

  14. Comparative Anatomy of Chromosomal Domains with Imprinted and Non-Imprinted Allele-Specific DNA Methylation

    OpenAIRE

    Paliwal, Anupam; Temkin, Alexis M.; Kerkel, Kristi; Yale, Alexander; Yotova, Iveta; Drost, Natalia; Lax, Simon; Nhan-Chang, Chia-Ling; Powell, Charles; Borczuk, Alain; Aviv, Abraham; Wapner, Ronald; Chen, Xiaowei; Nagy, Peter L.; Schork, Nicholas,

    2013-01-01

    Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault R...

  15. Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

    OpenAIRE

    Anupam Paliwal; Temkin, Alexis M.; Kristi Kerkel; Alexander Yale; Iveta Yotova; Natalia Drost; Simon Lax; Chia-Ling Nhan-Chang; Charles Powell; Alain Borczuk; Abraham Aviv; Ronald Wapner; Xiaowei Chen; Nagy, Peter L.; Nicholas Schork

    2013-01-01

    Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault R...

  16. Genome-wide survey of allele-specific splicing in humans

    Directory of Open Access Journals (Sweden)

    Scheffler Konrad

    2008-06-01

    Full Text Available Abstract Background Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a mutation on the efficiency of mRNA splicing is often difficult to predict, many mutations that cause disease through an effect on splicing are likely to remain undiscovered. Results We have combined a genome-wide scan for sequence polymorphisms likely to affect mRNA splicing with analysis of publicly available Expressed Sequence Tag (EST and exon array data. The genome-wide scan uses published tools and identified 30,977 SNPs located within donor and acceptor splice sites, branch points and exonic splicing enhancer elements. For 1,185 candidate splicing polymorphisms the difference in splicing between alternative alleles was corroborated by publicly available exon array data from 166 lymphoblastoid cell lines. We developed a novel probabilistic method to infer allele-specific splicing from EST data. The method uses SNPs and alternative mRNA isoforms mapped to EST sequences and models both regulated alternative splicing as well as allele-specific splicing. We have also estimated heritability of splicing and report that a greater proportion of genes show evidence of splicing heritability than show heritability of overall gene expression level. Our results provide an extensive resource that can be used to assess the possible effect on splicing of human polymorphisms in putative splice-regulatory sites. Conclusion We report a set of genes showing evidence of allele-specific splicing from an integrated analysis of genomic polymorphisms, EST data and exon array

  17. Visualizing allele-specific expression in single cells reveals epigenetic mosaicism in an H19 loss-of-imprinting mutant.

    Science.gov (United States)

    Ginart, Paul; Kalish, Jennifer M; Jiang, Connie L; Yu, Alice C; Bartolomei, Marisa S; Raj, Arjun

    2016-03-01

    Imprinting is a classic mammalian epigenetic phenomenon that results in expression from a single parental allele. Imprinting defects can lead to inappropriate expression from the normally silenced allele, but it remains unclear whether every cell in a mutant organism follows the population average, which would have profound implications for human imprinting disorders. Here, we apply a new fluorescence in situ hybridization method that measures allele-specific expression in single cells to address this question in mutants exhibiting aberrant H19/Igf2 (insulin-like growth factor 2) imprinting. We show that mutant primary embryonic mouse fibroblasts are comprised of two subpopulations: one expressing both H19 alleles and another expressing only the maternal copy. Only in the latter cell population is Igf2 expression detected. Furthermore, the two subpopulations are stable in that cells do not interconvert between the two expression patterns. Combined small input methylation analysis and transcriptional imaging revealed that these two mutant subpopulations exhibit distinct methylation patterns at their imprinting control regions. Consistently, pharmacological inhibition of DNA methylation reduced the proportion of monoallelic cells. Importantly, we observed that the same two subpopulations are also present in vivo within murine cardiac tissue. Our results establish that imprinting disorders can display striking single-cell heterogeneity in their molecular phenotypes and suggest that such heterogeneity may underlie epigenetic mosaicism in human imprinting disorders.

  18. Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.

    OpenAIRE

    Wu, D Y; Ugozzoli, L; B..K. Pal; Wallace, R B

    1989-01-01

    A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, ligation, or restriction enzyme cleavage. Two allele-specific oligonucleotide primers, one specific for the sickle cell allele and one specific for the normal allele, together with another primer co...

  19. No association between germline allele-specific expression of TGFBR1 and colorectal cancer risk in Caucasian and Ashkenazi populations

    OpenAIRE

    Seguí, N; Stevens, K. N.; Guinó, E.; Rozek, L S; Moreno, V R; Capellá, G; Gruber, S B; Valle, L.

    2011-01-01

    Background: Germline allele-specific expression (ASE) of the TGFBR1 gene has been reported as a strong risk factor for colorectal cancer (CRC) with an odds ratio close to 9. Considering the potential implications of the finding, we undertook the task of validating the initial results in this study. Methods: Allele-specific expression was measured using the highly quantitative and robust technique of pyrosequencing. Individuals from two different populations were studied, one Caucasian-dominat...

  20. Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

    Directory of Open Access Journals (Sweden)

    Anupam Paliwal

    2013-08-01

    Full Text Available Allele-specific DNA methylation (ASM is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons, one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault RNA in band 5q31, proved to be new examples of imprinted DMRs (maternal alleles methylated while a third, between STEAP3 and C2orf76 in chromosome band 2q14, showed non-imprinted haplotype-dependent ASM. Using long-read bisulfite sequencing (bis-seq in 8 human tissues we found that in all 3 domains the ASM is restricted to single differentially methylated regions (DMRs, each less than 2kb. The ASM in the C3orf27-RPN1 intergenic region was placenta-specific and associated with allele-specific expression of a long non-coding RNA. Strikingly, the discrete DMRs in all 3 regions overlap with binding sites for the insulator protein CTCF, which we found selectively bound to the unmethylated allele of the STEAP3-C2orf76 DMR. Methylation mapping in two additional genes with non-imprinted haplotype-dependent ASM, ELK3 and CYP2A7, showed that the CYP2A7 DMR also overlaps a CTCF site. Thus, two features of imprinted domains, highly localized DMRs and allele-specific insulator occupancy by CTCF, can also be found in chromosomal domains with non-imprinted ASM. Arguing for biological importance, our analysis of published whole genome bis-seq data from hES cells revealed multiple genome-wide association study (GWAS peaks near CTCF binding sites with ASM.

  1. Comparative anatomy of chromosomal domains with imprinted and non-imprinted allele-specific DNA methylation.

    Science.gov (United States)

    Paliwal, Anupam; Temkin, Alexis M; Kerkel, Kristi; Yale, Alexander; Yotova, Iveta; Drost, Natalia; Lax, Simon; Nhan-Chang, Chia-Ling; Powell, Charles; Borczuk, Alain; Aviv, Abraham; Wapner, Ronald; Chen, Xiaowei; Nagy, Peter L; Schork, Nicholas; Do, Catherine; Torkamani, Ali; Tycko, Benjamin

    2013-08-01

    Allele-specific DNA methylation (ASM) is well studied in imprinted domains, but this type of epigenetic asymmetry is actually found more commonly at non-imprinted loci, where the ASM is dictated not by parent-of-origin but instead by the local haplotype. We identified loci with strong ASM in human tissues from methylation-sensitive SNP array data. Two index regions (bisulfite PCR amplicons), one between the C3orf27 and RPN1 genes in chromosome band 3q21 and the other near the VTRNA2-1 vault RNA in band 5q31, proved to be new examples of imprinted DMRs (maternal alleles methylated) while a third, between STEAP3 and C2orf76 in chromosome band 2q14, showed non-imprinted haplotype-dependent ASM. Using long-read bisulfite sequencing (bis-seq) in 8 human tissues we found that in all 3 domains the ASM is restricted to single differentially methylated regions (DMRs), each less than 2kb. The ASM in the C3orf27-RPN1 intergenic region was placenta-specific and associated with allele-specific expression of a long non-coding RNA. Strikingly, the discrete DMRs in all 3 regions overlap with binding sites for the insulator protein CTCF, which we found selectively bound to the unmethylated allele of the STEAP3-C2orf76 DMR. Methylation mapping in two additional genes with non-imprinted haplotype-dependent ASM, ELK3 and CYP2A7, showed that the CYP2A7 DMR also overlaps a CTCF site. Thus, two features of imprinted domains, highly localized DMRs and allele-specific insulator occupancy by CTCF, can also be found in chromosomal domains with non-imprinted ASM. Arguing for biological importance, our analysis of published whole genome bis-seq data from hES cells revealed multiple genome-wide association study (GWAS) peaks near CTCF binding sites with ASM. PMID:24009515

  2. Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic

    Directory of Open Access Journals (Sweden)

    Eleonidas Moura Lima

    2015-06-01

    Full Text Available Gastric cancer (GC is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP rs1695, in the Glutathione S-Transferase Pi (GSTP1 gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P 0.05. These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.

  3. Efficient allele-specific targeting of LRRK2 R1441 mutations mediated by RNAi.

    Directory of Open Access Journals (Sweden)

    Laura de Yñigo-Mojado

    Full Text Available Since RNA interference (RNAi has the potential to discriminate between single nucleotide changes, there is growing interest in the use of RNAi as a promising therapeutical approach to target dominant disease-associated alleles. Mutations in the leucine-rich repeat kinase 2 (LRRK2 gene have been linked to dominantly inherited Parkinson's disease (PD. We focused on three LRRK2 mutations (R1441G/C and the more prevalent G2109S hoping to identify shRNAs that would both recognize and efficiently silence the mutated alleles preferentially over the wild-type alleles. Using a luciferase-based reporter system, we identified shRNAs that were able to specifically target the R1441G and R1441C alleles with 80% silencing efficiency. The same shRNAs were able to silence specifically mRNAs encoding either partial or full-length mutant LRRK2 fusion proteins, while having a minimal effect on endogenous wild-type LRRK2 expression when transfected in 293FT cells. Shifting of the mutant recognition site (MRS from position 11 to other sites (4 and 16, within the 19-mer window of our shRNA design reduced specificity and overall silencing efficiency. Developing an allele-specific RNAi of G2019S was problematic. Placement of the MRS at position 10 resulted in efficient silencing of reporters (75-80%, but failed to discriminate between mutant and wild-type alleles. Shifting of the MRS to positions 4, 5, 15, 16 increased the specificity of the shRNAs, but reduced the overall silencing efficiency. Consistent with previous reports, these data confirm that MRS placement influences both allele-specificity and silencing strength of shRNAs, while further modification to hairpin design or MRS position may lead to the development of effective G2019S shRNAs. In summary, the effective shRNA against LRRK2 R1441 alleles described herein suggests that RNAi-based therapy of inherited Parkinson's disease is a viable approach towards developing effective therapeutic interventions for

  4. Hybrid sterility and evolution in Hawaiian Drosophila: differential gene and allele-specific expression analysis of backcross males.

    Science.gov (United States)

    Brill, E; Kang, L; Michalak, K; Michalak, P; Price, D K

    2016-08-01

    The Hawaiian Drosophila are an iconic example of sequential colonization, adaptive radiation and speciation on islands. Genetic and phenotypic analysis of closely related species pairs that exhibit incomplete reproductive isolation can provide insights into the mechanisms of speciation. Drosophila silvestris from Hawai'i Island and Drosophila planitibia from Maui are two closely related allopatric Hawaiian picture-winged Drosophila that produce sterile F1 males but fertile F1 females, a pattern consistent with Haldane's rule. Backcrossing F1 hybrid females between these two species to parental species gives rise to recombinant males with three distinct sperm phenotypes despite a similar genomic background: motile sperm, no sperm (sterile), and immotile sperm. We found that these three reproductive morphologies of backcross hybrid males produce divergent gene expression profiles in testes, as measured with RNA sequencing. There were a total of 71 genes significantly differentially expressed between backcross males with no sperm compared with those backcross males with motile sperm and immotile sperm, but no significant differential gene expression between backcross males with motile sperm and backcross males with immotile sperm. All of these genes were underexpressed in males with no sperm, including a number of genes with previously known activities in adult testis. An allele-specific expression analysis showed overwhelmingly more cis-divergent than trans-divergent genes, with no significant difference in the ratio of cis- and trans-divergent genes among the sperm phenotypes. Overall, the results indicate that the regulation of gene expression involved in sperm production likely diverged relatively rapidly between these two closely related species. PMID:27220308

  5. Imprinted chromosomal domains revealed by allele-specific replication timing of the GABRB3 and GABRA5 genes

    Energy Technology Data Exchange (ETDEWEB)

    LaSalle, J.; Flint, A.; Lalande, M. [Harvard Medical School, Boston, MA (United States)] [and others

    1994-09-01

    The GABRB3 and GABRA5 genes are organized as a cluster in chromosome 15q11-q13. The genes are separated by around 100 kb and arranged in opposite transcriptional orientations. The GABA{sub A} receptor cluster lies near the Angelman and Prader-Willi loci and displays asynchronous DNA replication, suggesting that this region is subject to parental imprinting. In order to further study the association between DNA replication and imprinting, allele-specific replication was assayed by fluorescence in situ hybridization with {lambda}-phage probes from the GABRB3/A5 region and a D15Z1 satellite probe to identify the parental origin of each chromosome. The replication kinetics of each allele was determined by using a flow sorter to fractionate mitogen-stimulated lymphocytes on the basis of cell cycle progression prior to FISH analysis. These kinetic studies reveal a 50-150 kb chromosomal domain extending from the middle of the GABRB3/A5 intergenic region into the GABRA5 5{prime}-UTR which displays maternal replication in early S with paternal replication delayed until the end of S. In contrast, genomic regions on either side of this maternal early replication domain exhibit the opposite pattern with paternal before maternal replication and both alleles replicating in the latter half of S. These results indicate that the GABRB3/A5 region is divided into domains in which replication timing is determined by parental origin. In addition to a loss of asynchronous replication, organization into replication timing domains is also lost in lymphocytes from maternal and paternal uniparental disomy 15 patients suggesting that a chromosome contribution from both parents is required for the establishment of the imprinted replication domains.

  6. Efficient and allele-specific genome editing of disease loci in human iPSCs.

    Science.gov (United States)

    Smith, Cory; Abalde-Atristain, Leire; He, Chaoxia; Brodsky, Brett R; Braunstein, Evan M; Chaudhari, Pooja; Jang, Yoon-Young; Cheng, Linzhao; Ye, Zhaohui

    2015-03-01

    Efficient and precise genome editing is crucial for realizing the full research and therapeutic potential of human induced pluripotent stem cells (iPSCs). Engineered nucleases including CRISPR/Cas9 and transcription activator like effector nucleases (TALENs) provide powerful tools for enhancing gene-targeting efficiency. In this study, we investigated the relative efficiencies of CRISPR/Cas9 and TALENs in human iPSC lines for inducing both homologous donor-based precise genome editing and nonhomologous end joining (NHEJ)-mediated gene disruption. Significantly higher frequencies of NHEJ-mediated insertions/deletions were detected at several endogenous loci using CRISPR/Cas9 than using TALENs, especially at nonexpressed targets in iPSCs. In contrast, comparable efficiencies of inducing homologous donor-based genome editing were observed at disease-associated loci in iPSCs. In addition, we investigated the specificity of guide RNAs used in the CRISPR/Cas9 system in targeting disease-associated point mutations in patient-specific iPSCs. Using myeloproliferative neoplasm patient-derived iPSCs that carry an acquired JAK2-V617F point mutation and α1-antitrypsin (AAT) deficiency patient-derived iPSCs that carry an inherited Z-AAT point mutation, we demonstrate that Cas9 can specifically target either the mutant or the wild-type allele with little disruption at the other allele differing by a single nucleotide. Overall, our results demonstrate the advantages of the CRISPR/Cas9 system in allele-specific genome targeting and in NHEJ-mediated gene disruption.

  7. Development of allele-specific therapeutic siRNA in Meesmann epithelial corneal dystrophy.

    Directory of Open Access Journals (Sweden)

    Haihui Liao

    Full Text Available BACKGROUND: Meesmann epithelial corneal dystrophy (MECD is an inherited eye disorder caused by dominant-negative mutations in either keratins K3 or K12, leading to mechanical fragility of the anterior corneal epithelium, the outermost covering of the eye. Typically, patients suffer from lifelong irritation of the eye and/or photophobia but rarely lose visual acuity; however, some individuals are severely affected, with corneal scarring requiring transplant surgery. At present no treatment exists which addresses the underlying pathology of corneal dystrophy. The aim of this study was to design and assess the efficacy and potency of an allele-specific siRNA approach as a future treatment for MECD. METHODS AND FINDINGS: We studied a family with a consistently severe phenotype where all affected persons were shown to carry heterozygous missense mutation Leu132Pro in the KRT12 gene. Using a cell-culture assay of keratin filament formation, mutation Leu132Pro was shown to be significantly more disruptive than the most common mutation, Arg135Thr, which is associated with typical, mild MECD. A siRNA sequence walk identified a number of potent inhibitors for the mutant allele, which had no appreciable effect on wild-type K12. The most specific and potent inhibitors were shown to completely block mutant K12 protein expression with negligible effect on wild-type K12 or other closely related keratins. Cells transfected with wild-type K12-EGFP construct show a predominantly normal keratin filament formation with only 5% aggregate formation, while transfection with mutant K12-EGFP construct resulted in a significantly higher percentage of keratin aggregates (41.75%; p<0.001 with 95% confidence limits. The lead siRNA inhibitor significantly rescued the ability to form keratin filaments (74.75% of the cells contained normal keratin filaments; p<0.001 with 95% confidence limits. CONCLUSIONS: This study demonstrates that it is feasible to design highly potent si

  8. Citrus (Rutaceae) SNP Markers Based on Competitive Allele-Specific PCR; Transferability Across the Aurantioideae Subfamily

    OpenAIRE

    Andres Garcia-Lor; Gema Ancillo; Luis Navarro; Patrick Ollitrault

    2013-01-01

    Premise of the study: Single nucleotide polymorphism (SNP) markers based on Competitive Allele-Specific PCR (KASPar) were developed from sequences of three Citrus species. Their transferability was tested in 63 Citrus genotypes and 19 relative genera of the subfamily Aurantioideae to estimate the potential of SNP markers, selected from a limited intrageneric discovery panel, for ongoing broader diversity analysis at the intra- and intergeneric levels and systematic germplasm bank characteriza...

  9. SNPsplit: Allele-specific splitting of alignments between genomes with known SNP genotypes [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Felix Krueger

    2016-06-01

    Full Text Available Sequencing reads overlapping polymorphic sites in diploid mammalian genomes may be assigned to one allele or the other. This holds the potential to detect gene expression, chromatin modifications, DNA methylation or nuclear interactions in an allele-specific fashion. SNPsplit is an allele-specific alignment sorter designed to read files in SAM/BAM format and determine the allelic origin of reads or read-pairs that cover known single nucleotide polymorphic (SNP positions. For this to work libraries must have been aligned to a genome in which all known SNP positions were masked with the ambiguity base ’N’ and aligned using a suitable mapping program such as Bowtie2, TopHat, STAR, HISAT2, HiCUP or Bismark. SNPsplit also provides an automated solution to generate N-masked reference genomes for hybrid mouse strains based on the variant call information provided by the Mouse Genomes Project. The unique ability of SNPsplit to work with various different kinds of sequencing data including RNA-Seq, ChIP-Seq, Bisulfite-Seq or Hi-C opens new avenues for the integrative exploration of allele-specific data.

  10. SNPsplit: Allele-specific splitting of alignments between genomes with known SNP genotypes [version 2; referees: 3 approved

    Directory of Open Access Journals (Sweden)

    Felix Krueger

    2016-07-01

    Full Text Available Sequencing reads overlapping polymorphic sites in diploid mammalian genomes may be assigned to one allele or the other. This holds the potential to detect gene expression, chromatin modifications, DNA methylation or nuclear interactions in an allele-specific fashion. SNPsplit is an allele-specific alignment sorter designed to read files in SAM/BAM format and determine the allelic origin of reads or read-pairs that cover known single nucleotide polymorphic (SNP positions. For this to work libraries must have been aligned to a genome in which all known SNP positions were masked with the ambiguity base 'N' and aligned using a suitable mapping program such as Bowtie2, TopHat, STAR, HISAT2, HiCUP or Bismark. SNPsplit also provides an automated solution to generate N-masked reference genomes for hybrid mouse strains based on the variant call information provided by the Mouse Genomes Project. The unique ability of SNPsplit to work with various different kinds of sequencing data including RNA-Seq, ChIP-Seq, Bisulfite-Seq or Hi-C opens new avenues for the integrative exploration of allele-specific data.

  11. Allele-specific transcription factor binding to common and rare variants associated with disease and gene expression.

    Science.gov (United States)

    Cavalli, Marco; Pan, Gang; Nord, Helena; Wallerman, Ola; Wallén Arzt, Emelie; Berggren, Olof; Elvers, Ingegerd; Eloranta, Maija-Leena; Rönnblom, Lars; Lindblad Toh, Kerstin; Wadelius, Claes

    2016-05-01

    Genome-wide association studies (GWAS) have identified a large number of disease-associated SNPs, but in few cases the functional variant and the gene it controls have been identified. To systematically identify candidate regulatory variants, we sequenced ENCODE cell lines and used public ChIP-seq data to look for transcription factors binding preferentially to one allele. We found 9962 candidate regulatory SNPs, of which 16 % were rare and showed evidence of larger functional effect than common ones. Functionally rare variants may explain divergent GWAS results between populations and are candidates for a partial explanation of the missing heritability. The majority of allele-specific variants (96 %) were specific to a cell type. Furthermore, by examining GWAS loci we found >400 allele-specific candidate SNPs, 141 of which were highly relevant in our cell types. Functionally validated SNPs support identification of an SNP in SYNGR1 which may expose to the risk of rheumatoid arthritis and primary biliary cirrhosis, as well as an SNP in the last intron of COG6 exposing to the risk of psoriasis. We propose that by repeating the ChIP-seq experiments of 20 selected transcription factors in three to ten people, the most common polymorphisms can be interrogated for allele-specific binding. Our strategy may help to remove the current bottleneck in functional annotation of the genome. PMID:26993500

  12. FACETS: allele-specific copy number and clonal heterogeneity analysis tool for high-throughput DNA sequencing

    Science.gov (United States)

    Shen, Ronglai; Seshan, Venkatraman E.

    2016-01-01

    Allele-specific copy number analysis (ASCN) from next generation sequencing (NGS) data can greatly extend the utility of NGS beyond the identification of mutations to precisely annotate the genome for the detection of homozygous/heterozygous deletions, copy-neutral loss-of-heterozygosity (LOH), allele-specific gains/amplifications. In addition, as targeted gene panels are increasingly used in clinical sequencing studies for the detection of ‘actionable’ mutations and copy number alterations to guide treatment decisions, accurate, tumor purity-, ploidy- and clonal heterogeneity-adjusted integer copy number calls are greatly needed to more reliably interpret NGS-based cancer gene copy number data in the context of clinical sequencing. We developed FACETS, an ASCN tool and open-source software with a broad application to whole genome, whole-exome, as well as targeted panel sequencing platforms. It is a fully integrated stand-alone pipeline that includes sequencing BAM file post-processing, joint segmentation of total- and allele-specific read counts, and integer copy number calls corrected for tumor purity, ploidy and clonal heterogeneity, with comprehensive output and integrated visualization. We demonstrate the application of FACETS using The Cancer Genome Atlas (TCGA) whole-exome sequencing of lung adenocarcinoma samples. We also demonstrate its application to a clinical sequencing platform based on a targeted gene panel. PMID:27270079

  13. Social media and mobile communications adoption patterns of South African civil society organisations

    Directory of Open Access Journals (Sweden)

    Kiru Pillay

    2014-03-01

    Full Text Available Background: The resurgence of civil society has largely been attributed to the sector’s ability to exploit new interactive technologies and its ability to adapt its communication and mobilisation strategies.Objectives: This study focuses on how South African civil society organisations (CSOs deploy Web 2.0 services and technologies for social advocacy and the context of this technology use.Whilst the literature points to many studies relating to the use of the Internet for advocacy, it also suggests that the role and impact of emerging technologies have not been studied in any detail in CSOs. Such studies have the potential to provide new perspectives to current theoretical frameworks and also to add to the discourse around the use of emerging technologies for advocacy.Method: A survey of South African CSOs explored the level of knowledge of social media services and revealed which services in particular were being adopted.Results: The key findings that emerged were that the sector has a low level of knowledge of social media services and an accompanying low level of adoption. These are partly explained by factors such as macro-economic policies and low levels of Internet penetration and ICT readiness.Conclusion: Further research to determine why certain social media services have been embraced more willingly than others and an analysis of the patterns of adoption to determine any underlying significance or relationships is necessary. An analysis of how CSOs build their advocacy capabilities by appropriating social media and how they thus provide alternate discourses and agendas would be instructive.

  14. Allele-Specific Transcriptome and Methylome Analysis Reveals Stable Inheritance and Cis-Regulation of DNA Methylation in Nasonia.

    Science.gov (United States)

    Wang, Xu; Werren, John H; Clark, Andrew G

    2016-07-01

    Gene expression divergence between closely related species could be attributed to both cis- and trans- DNA sequence changes during evolution, but it is unclear how the evolutionary dynamics of epigenetic marks are regulated. In eutherian mammals, biparental DNA methylation marks are erased and reset during gametogenesis, resulting in paternal or maternal imprints, which lead to genomic imprinting. Whether DNA methylation reprogramming exists in insects is not known. Wasps of the genus Nasonia are non-social parasitoids that are emerging as a model for studies of epigenetic processes in insects. In this study, we quantified allele-specific expression and methylation genome-wide in Nasonia vitripennis and Nasonia giraulti and their reciprocal F1 hybrids. No parent-of-origin effect in allelic expression was found for >8,000 covered genes, suggesting a lack of genomic imprinting in adult Nasonia. As we expected, both significant cis- and trans- effects are responsible for the expression divergence between N. vitripennis and N. giraulti. Surprisingly, all 178 differentially methylated genes are also differentially methylated between the two alleles in F1 hybrid offspring, recapitulating the parental methylation status with nearly 100% fidelity, indicating the presence of strong cis-elements driving the target of gene body methylation. In addition, we discovered that total and allele-specific expression are positively correlated with allele-specific methylation in a subset of the differentially methylated genes. The 100% cis-regulation in F1 hybrids suggests the methylation machinery is conserved and DNA methylation is targeted by cis features in Nasonia. The lack of genomic imprinting and parent-of-origin differentially methylated regions in Nasonia, together with the stable inheritance of methylation status between generations, suggests either a cis-regulatory motif for methylation at the DNA level or highly stable inheritance of an epigenetic signal in Nasonia. PMID

  15. A single tube modified allele-specific-PCR for rapid detection of erythromycin-resistant Mycoplasma pneumoniae in Beijing

    Institute of Scientific and Technical Information of China (English)

    LI Shao-li; SUN Hong-mei; ZHAO Han-qing; CAO Ling; YUAN Yi; FENG Yan-ling; XUE Guan-hua

    2012-01-01

    Background Mycoplasma pneumoniae (M.pneumoniae) is one of the common pathogens causing atypical pneumonia.In recent years,resistance to macrolides has become more common,especially in China.Previous studies have confirmed that the mutation at position 2063 in domain V of the 23S rRNA is the most prevalent,followed by the mutation at position 2064.Reported molecular detection methods for the identification of these mutations include direct sequencing,restriction fragment length polymorphism analysis,real-time polymerase chain reaction (PCR) with high-resolution melt analysis,and nested PCR-linked with capillary electrophoresis,etc.The most commonly used method for monitoring resistance-conferring mutations in M.pneumoniae is direct DNA sequencing of PCR or nested PCR products.However,these methods are time-consuming,labor-intensive or need expensive equipments.Therefore the development of rapid and sensitive methods is very important for monitoring the resistance globally.Methods In this study,we reported a fast and cost-effective method for detecting 2063 and/or 2064 macrolide resistant mutations from specimens using a modified allele-specific PCR analysis,and all results were compared with the sequencing data.We also analyzed the clinical courses of these samples to confirm the modified allele-specific PCR results.Results Among 97 M.pneumoniae specimens,88 were found to possess mutations by this method,and all modified allele-specific PCR analysis results were consistent with the sequencing data.The data of the clinical courses of these 97cases showed that they suffered from severe pneumonia.Erythromycin showed better efficacy on cases from which no macrolide resistance mutation was found on their specimens.However,in some cases from which mutations were detected,erythromycin monotherapy had poor efficacy,and on these patients severe symptoms improved only when azithromycin was added to the treatment.Conclusions The drug-resistant M.pneumoniae is very common in

  16. Use of allele-specific FAIRE to determine functional regulatory polymorphism using large-scale genotyping arrays

    DEFF Research Database (Denmark)

    Smith, Frank Andrew; Howard, Philip; Shah, Sonia;

    2012-01-01

    Following the widespread use of genome-wide association studies (GWAS), focus is turning towards identification of causal variants rather than simply genetic markers of diseases and traits. As a step towards a high-throughput method to identify genome-wide, non-coding, functional regulatory...... identified an allele-specific regulatory polymorphism within NR1H3 (coding for LXR-α), rs7120118, coinciding with a previously GWAS-identified SNP for HDL-C levels. This finding was confirmed using FAIRE-gen with the 200,000 SNP Illumina Metabochip and verified with the established method of TaqMan allelic...

  17. A simple and rapid method for HLA-DQA1 genotyping by digestion of PCR-amplified DNA with allele specific restriction endonucleases.

    Science.gov (United States)

    Maeda, M; Murayama, N; Ishii, H; Uryu, N; Ota, M; Tsuji, K; Inoko, H

    1989-11-01

    The second exon of the HLA-DQA1 genes was selectively amplified from genomic DNAs of 72 HLA-homozygous B cell lines by the polymerase chain reaction (PCR). Amplified DNAs were digested with HaeIII, Ddel, ScrFI, FokI and RsaI, which recognize allelic sequence variations in the polymorphic segments of the DQA1 second exon, and then subjected to electrophoresis in polyacrylamide gels. Eight different polymorphic patterns of restriction fragments were obtained, and seven were identical to patterns predicted from the known DNA sequences, correlating with each HLA-DQw type defined by serological typing. The remaining one pattern cannot be explained from the sequence data, suggesting the presence of a novel DQA1 allele at the nucleotide level. This PCR-RFLP method provides a simple and rapid technique for accurate definition of the HLA-DQ types at the nucleotide level, eliminating the need for radioisotope as well as allele specific oligonucleotide probes and can be extended and applied to HLA-DR, -Dw DP typing. PMID:2576477

  18. A Singular Vision for a Disparate Future: Technology Adoption Patterns in Higher Learning through 2035

    Science.gov (United States)

    Henshaw, Robert G.

    2008-01-01

    Technology adoption in any sector is rarely uniform. Understanding the drivers and constraints associated with technology adoption makes it easier to anticipate how technology will be used and what populations will benefit the most. Robert G. Henshaw examines factors likely to influence technology adoption within U.S. higher education over the…

  19. Polymorphism analysis of Chinese Theileria sergenti using allele-specific polymerase chain reaction of the major piroplasm surface protein gene.

    Science.gov (United States)

    Liu, Ai Hong; Guan, Gui Quan; Liu, Jun Long; Liu, Zhi Jie; Leblanc, Neil; Li, You Quan; Gao, Jin Liang; Ma, Mi Ling; Niu, Qing Li; Ren, Qiao Yun; Bai, Qi; Yin, Hong; Luo, Jian Xun

    2011-02-01

    Theileria sergenti is a tick-borne parasite found in many parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a marker for epidemiological and phylogenetic studies of benign Theileria species. In this study, Chinese species of T. sergenti were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the MPSP gene. Using universal or allele-specific primer sets for PCR amplification of the MPSP gene, 98 of 288 cattle blood samples, collected from 6 provinces in China, were found to be positive. Among the positive samples, only 3 allelic MPSP gene types (Chitose [C]-, Ikeda [I]-, and buffeli [B]-type) were successfully amplified. Moreover, the results revealed that the majority of the parasites sampled in this study were C- and I-type (prevalence of 84 and 69%, respectively), whereas the B-type was less common (prevalence of 36%). Co-infections with C-, I-, and B-type T. sergenti also were found. An additional known allele, Thai-type, was not detected. Phylogenetic analysis based on the MPSP gene sequences, including 3 standard stocks generated in the laboratory ( T. sergenti Wenchuan, T. sergenti Ningxian, and T. sergenti Liaoyang), revealed that the isolates of Chinese sergenti were comprised of at least 4 allelic MPSP gene types, i.e., C-, I-, B1-, and B2-type, and these parasites with 6 MPSP types 1-5 and 7 were present in China.

  20. Use of allele-specific FAIRE to determine functional regulatory polymorphism using large-scale genotyping arrays.

    Science.gov (United States)

    Smith, Andrew J P; Howard, Philip; Shah, Sonia; Eriksson, Per; Stender, Stefan; Giambartolomei, Claudia; Folkersen, Lasse; Tybjærg-Hansen, Anne; Kumari, Meena; Palmen, Jutta; Hingorani, Aroon D; Talmud, Philippa J; Humphries, Steve E

    2012-01-01

    Following the widespread use of genome-wide association studies (GWAS), focus is turning towards identification of causal variants rather than simply genetic markers of diseases and traits. As a step towards a high-throughput method to identify genome-wide, non-coding, functional regulatory variants, we describe the technique of allele-specific FAIRE, utilising large-scale genotyping technology (FAIRE-gen) to determine allelic effects on chromatin accessibility and regulatory potential. FAIRE-gen was explored using lymphoblastoid cells and the 50,000 SNP Illumina CVD BeadChip. The technique identified an allele-specific regulatory polymorphism within NR1H3 (coding for LXR-α), rs7120118, coinciding with a previously GWAS-identified SNP for HDL-C levels. This finding was confirmed using FAIRE-gen with the 200,000 SNP Illumina Metabochip and verified with the established method of TaqMan allelic discrimination. Examination of this SNP in two prospective Caucasian cohorts comprising 15,000 individuals confirmed the association with HDL-C levels (combined beta = 0.016; p = 0.0006), and analysis of gene expression identified an allelic association with LXR-α expression in heart tissue. Using increasingly comprehensive genotyping chips and distinct tissues for examination, FAIRE-gen has the potential to aid the identification of many causal SNPs associated with disease from GWAS. PMID:22916038

  1. Use of allele-specific FAIRE to determine functional regulatory polymorphism using large-scale genotyping arrays.

    Directory of Open Access Journals (Sweden)

    Andrew J P Smith

    Full Text Available Following the widespread use of genome-wide association studies (GWAS, focus is turning towards identification of causal variants rather than simply genetic markers of diseases and traits. As a step towards a high-throughput method to identify genome-wide, non-coding, functional regulatory variants, we describe the technique of allele-specific FAIRE, utilising large-scale genotyping technology (FAIRE-gen to determine allelic effects on chromatin accessibility and regulatory potential. FAIRE-gen was explored using lymphoblastoid cells and the 50,000 SNP Illumina CVD BeadChip. The technique identified an allele-specific regulatory polymorphism within NR1H3 (coding for LXR-α, rs7120118, coinciding with a previously GWAS-identified SNP for HDL-C levels. This finding was confirmed using FAIRE-gen with the 200,000 SNP Illumina Metabochip and verified with the established method of TaqMan allelic discrimination. Examination of this SNP in two prospective Caucasian cohorts comprising 15,000 individuals confirmed the association with HDL-C levels (combined beta = 0.016; p = 0.0006, and analysis of gene expression identified an allelic association with LXR-α expression in heart tissue. Using increasingly comprehensive genotyping chips and distinct tissues for examination, FAIRE-gen has the potential to aid the identification of many causal SNPs associated with disease from GWAS.

  2. Pre-teen cell phone adoption: consequences for later patterns of phone usage and involvement

    OpenAIRE

    Geser, Hans

    2006-01-01

    Contents: 1. Introduction; 2. The particular proneness of adolescents to use mobile phones; 3. The consequences of earlier adoption as a focus of research; 4. Data and Methodology; 5. Empirical results; 5. 1.The rapid trend toward ever earlier initial adoption; 5.2 Intensity of phone usage; 5.3 Extensity and intensity of phone partner networks; 5.4 Temporal accessibility; 5.5 Affective mobile phone involvement; 5.6 The changes of early adoption effects with increasing age; 5.7 Early adoption ...

  3. Ploidy mosaicism and allele-specific gene expression differences in the allopolyploid Squalius alburnoides

    Directory of Open Access Journals (Sweden)

    Matos Isa

    2011-12-01

    Full Text Available Abstract Background Squalius alburnoides is an Iberian cyprinid fish resulting from an interspecific hybridisation between Squalius pyrenaicus females (P genome and males of an unknown Anaecypris hispanica-like species (A genome. S. alburnoides is an allopolyploid hybridogenetic complex, which makes it a likely candidate for ploidy mosaicism occurrence, and is also an interesting model to address questions about gene expression regulation and genomic interactions. Indeed, it was previously suggested that in S. alburnoides triploids (PAA composition silencing of one of the three alleles (mainly of the P allele occurs. However, not a whole haplome is inactivated but a more or less random inactivation of alleles varying between individuals and even between organs of the same fish was seen. In this work we intended to correlate expression differences between individuals and/or between organs to the occurrence of mosaicism, evaluating if mosaics could explain previous observations and its impact on the assessment of gene expression patterns. Results To achieve our goal, we developed flow cytometry and cell sorting protocols for this system generating more homogenous cellular and transcriptional samples. With this set-up we detected 10% ploidy mosaicism within the S. alburnoides complex, and determined the allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin in cells from liver and kidney of mosaic and non-mosaic individuals coming from different rivers over a wide geographic range. Conclusions Ploidy mosaicism occurs sporadically within the S. alburnoides complex, but in a frequency significantly higher than reported for other organisms. Moreover, we could exclude the influence of this phenomenon on the detection of variable allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin in cells from liver and kidney of triploid individuals. Finally, we determined that the expression patterns

  4. Aberrant allele-specific replication, independent of parental origin, in blood cells of cancer patients

    International Nuclear Information System (INIS)

    Allelic counterparts of biallelically expressed genes display an epigenetic symmetry normally manifested by synchronous replication, different from genes subjected to monoallelic expression, which normally are characterized by an asynchronous mode of replication (well exemplified by the SNRPN imprinted locus). Malignancy was documented to be associated with gross modifications in the inherent replication-timing coordination between allelic counterparts of imprinted genes as well as of biallelically expressed loci. The cancer-related allelic replication timing aberrations are non-disease specific and appear in peripheral blood cells of cancer patients, including those with solid tumors. As such they offer potential blood markers for non-invasive cancer test. The present study was aimed to gain some insight into the mechanism leading to the replication timing alterations of genes in blood lymphocytes of cancer patients. Peripheral blood samples derived from patients with prostate cancer were chosen to represent the cancerous status, and samples taken from patients with no cancer but with benign prostate hyperplasia were used to portray the normal status. Fluorescence In Situ Hybridization (FISH) replication assay, applied to phytohemagglutinin (PHA)-stimulated blood lymphocytes, was used to evaluate the temporal order (either synchronous or asynchronous) of genes in the patients' cells. We demonstrated that: (i) the aberrant epigenetic profile, as delineated by the cancer status, is a reversible modification, evidenced by our ability to restore the normal patterns of replication in three unrelated loci (CEN15, SNRPN and RB1) by introducing an archetypical demethylating agent, 5-azacytidine; (ii) following the rehabilitating effect of demethylation, an imprinted gene (SNRPN) retains its original parental imprint; and (iii) the choice of an allele between early or late replication in the aberrant asynchronous replication, delineated by the cancer status, is not

  5. BRCA1 185delAG MUTATION CAN BE EASILY DETECTED BY AN ADAPTED ALLELE-SPECIFIC PCR

    Directory of Open Access Journals (Sweden)

    Anca Negura

    2012-03-01

    Full Text Available BRCA1 gene accounts for a majority of hereditary breast and ovarian cancers. Germinal deleteriousmutations within this gene are directly responsible for the disease, with a lifetime risk of cancer for mutations carriers ofabout 80%. While outbred and western populations usually show a heterogeneous profile of unique and familialmutations, in isolated and eastern European populations some recurrent mutations can be afforded the most responsibilityfor familial hereditary cases. In Ashkenazi Jewish and most Slavic eastern population, the BRCA1 185delAG is one of themost frequent mutations. Therefore, rapid screening by PCR-based methods can be useful in oncogenetic diagnosis. Herewe present implementation of an adapted allele-specific PCR for the detection of 185delAG, with wide applications indiagnosis and genotyping for large population groups.

  6. A Novel Real-time Fluorescence Mutant-allele-specific Amplification Method for Rapid Single Nucleotide Polymorphism Analysis

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Current methods for single nucleotide polymorphism (SNP) analysis are timeconsuming and complicated. We aimed at development of one-step real-time fluorescence mutant-allele-specific amplification (MASA) method for rapid SNP analysis. The method is a marriage of two technologies: MASA primers for target DNA and a double-stranded DNA-selective fluorescent dye, SYBR Green I. Genotypes are separated according to the different threshold cycles of the wild-type and mutant primers. K-ras oncogene was used as a target to validate the feasibility of the method. The experimental results showed that the different genotypes can be clearly discriminated by the assay. The real-time fluorescence MASA method will have an enormous potential for fast and reliable SNP analysis due to its simplicity and low cost.

  7. An allele-specific polymerase chain reaction assay for the differentiation of members of the Anopheles culicifacies complex

    Indian Academy of Sciences (India)

    O P Singh; Geeta Goswami; N Nanda; K Raghavendra; D Chandra; S K Subbarao

    2004-09-01

    Anopheles culicifacies, the principal vector of malaria in India, is a complex of five cryptic species which are morphologically indistinguishable at any stage of life. In view of the practical difficulties associated with classical cytotaxonomic method for the identification of members of the complex, an allele-specific polymerase chain reaction (ASPCR) assay targeted to the D3 domain of 28S ribosomal DNA was developed. The assay discriminates An. culicifacies species A and D from species B, C and E. The assay was validated using chromosomally-identified specimens of An. culicifacies from different geographical regions of India representing different sympatric associations. The assay correctly differentiates species A and D from species B, C and E. The possible use of this diagnostic assay in disease vector control programmes is discussed.

  8. Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny

    Science.gov (United States)

    Cuenca, José; Aleza, Pablo; Navarro, Luis; Ollitrault, Patrick

    2013-01-01

    Background Polyploidy is a major component of eukaryote evolution. Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole-genome coverage of single nucleotide polymorphism (SNP) markers, it is essential to implement a versatile SNP genotyping method to assign allelic configuration efficiently in polyploids. Scope This work evaluates the usefulness of the KASPar method, based on competitive allele-specific PCR, for the assignment of SNP allelic configuration. Citrus was chosen as a model because of its economic importance, the ongoing worldwide polyploidy manipulation projects for cultivar and rootstock breeding, and the increasing availability of SNP markers. Conclusions Fifteen SNP markers were successfully designed that produced clear allele signals that were in agreement with previous genotyping results at the diploid level. The analysis of DNA mixes between two haploid lines (Clementine and pummelo) at 13 different ratios revealed a very high correlation (average = 0·9796; s.d. = 0·0094) between the allele ratio and two parameters [θ angle = tan−1 (y/x) and y′ = y/(x + y)] derived from the two normalized allele signals (x and y) provided by KASPar. Separated cluster analysis and analysis of variance (ANOVA) from mixed DNA simulating triploid and tetraploid hybrids provided 99·71 % correct allelic configuration. Moreover, triploid populations arising from 2n gametes and interploid crosses were easily genotyped and provided useful genetic information. This work demonstrates that the KASPar SNP genotyping technique is an efficient way to assign heterozygous allelic configurations within polyploid populations. This method is accurate, simple and cost-effective. Moreover, it may be useful for quantitative studies, such as relative allele-specific expression analysis and bulk segregant analysis

  9. Exploring associations between micro-level models of innovation diffusion and emerging macro-level adoption patterns

    Science.gov (United States)

    Laciana, Carlos E.; Rovere, Santiago L.; Podestá, Guillermo P.

    2013-04-01

    A micro-level agent-based model of innovation diffusion was developed that explicitly combines (a) an individual's perception of the advantages or relative utility derived from adoption, and (b) social influence from members of the individual's social network. The micro-model was used to simulate macro-level diffusion patterns emerging from different configurations of micro-model parameters. Micro-level simulation results matched very closely the adoption patterns predicted by the widely-used Bass macro-level model (Bass, 1969 [1]). For a portion of the p-q domain, results from micro-simulations were consistent with aggregate-level adoption patterns reported in the literature. Induced Bass macro-level parameters p and q responded to changes in micro-parameters: (1) p increased with the number of innovators and with the rate at which innovators are introduced; (2) q increased with the probability of rewiring in small-world networks, as the characteristic path length decreases; and (3) an increase in the overall perceived utility of an innovation caused a corresponding increase in induced p and q values. Understanding micro to macro linkages can inform the design and assessment of marketing interventions on micro-variables-or processes related to them-to enhance adoption of future products or technologies.

  10. Adoption Patterns of Faculty Who Integrate Computer Technology for Teaching and Learning in Higher Education.

    Science.gov (United States)

    Jacobsen, D. Michele

    This paper reports on research that builds and extends a diffusion of innovations framework and adopter categories in order to accurately reflect and describe faculty innovativeness with technology for teaching and learning. A World Wide Web-based survey was used to collect information from 76 faculty members from across disciplines at two major…

  11. Agencification and marketization in Danish and Swedish state administration - patterns of adoption

    DEFF Research Database (Denmark)

    Hansen, Morten Balle; Niklasson, Birgitta

    (Meyer, Boli, Thomas, and Ramirez 1997) and the notion of local path dependencies and tradition enforcing difference (March 1994; Premfors 1998) we elaborate hypotheses concerning the adoption of agencification practices in the state agencies of the two countries. The empirical analysis is based...

  12. Understanding the technology of computer technology diffusion: explaining computer adoption patterns and implications for the wage structure

    OpenAIRE

    Borghans, L.; ter Weel, B.J.

    2008-01-01

    We review the empirical literature about the implications of the computerization of the labor market to see whether it can explain observed computer adoption patterns and (long-term) changes in the wage structure. Evidence from empirical micro-economic studies turns out to be inconsistent with macro-economic studies that are based on Constant Elasticity of Substitution (CES) production functions. We propose a micro-economic foundation for the CES production function that allows for changes in...

  13. Ribosomal protein genes are highly enriched among genes with allele-specific expression in the interspecific F1 hybrid catfish.

    Science.gov (United States)

    Chen, Ailu; Wang, Ruijia; Liu, Shikai; Peatman, Eric; Sun, Luyang; Bao, Lisui; Jiang, Chen; Li, Chao; Li, Yun; Zeng, Qifan; Liu, Zhanjiang

    2016-06-01

    Interspecific hybrids provide a rich source for the analysis of allele-specific expression (ASE). In this work, we analyzed ASE in F1 hybrid catfish using RNA-Seq datasets. While the vast majority of genes were expressed with both alleles, 7-8 % SNPs exhibited significant differences in allele ratios of expression. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5420 (8.2 %) and 13,390 (7.5 %) SNPs were identified as significant ASE-SNPs, respectively. With these SNPs, a total of 1519 and 3075 ASE-genes were identified. Gene Ontology analysis revealed that genes encoding cytoplasmic ribosomal proteins (RP) were highly enriched among ASE genes. Parent-of-origin was determined for 27 and 30 ASE RP genes in the liver and gill, respectively. The results indicated that genes from both channel catfish and blue catfish were involved in ASE. However, each RP gene appeared to be almost exclusively expressed from only one parent, indicating that ribosomes in the hybrid catfish were in the "hybrid" form. Overall representation of RP transcripts among the transcriptome appeared lower in the F1 hybrid catfish than in channel catfish or blue catfish, suggesting that the "hybrid" ribosomes may work more efficiently for translation in the F1 hybrid catfish. PMID:26747053

  14. Allele-Specific Reduction of the Mutant Huntingtin Allele Using Transcription Activator-Like Effectors in Human Huntington's Disease Fibroblasts.

    Science.gov (United States)

    Fink, Kyle D; Deng, Peter; Gutierrez, Josh; Anderson, Joseph S; Torrest, Audrey; Komarla, Anvita; Kalomoiris, Stefanos; Cary, Whitney; Anderson, Johnathon D; Gruenloh, William; Duffy, Alexandra; Tempkin, Teresa; Annett, Geralyn; Wheelock, Vicki; Segal, David J; Nolta, Jan A

    2016-01-01

    Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by an abnormal expansion of CAG repeats. Although pathogenesis has been attributed to this polyglutamine expansion, the underlying mechanisms through which the huntingtin protein functions have yet to be elucidated. It has been suggested that postnatal reduction of mutant huntingtin through protein interference or conditional gene knockout could prove to be an effective therapy for patients suffering from HD. For allele-specific targeting, transcription activator-like effectors (TALE) were designed to target single-nucleotide polymorphisms (SNP) in the mutant allele and packaged into a vector backbone containing KRAB to promote transcriptional repression of the disease-associated allele. Additional TALEs were packaged into a vector backbone containing heterodimeric FokI and were designed to be used as nucleases (TALEN) to cause a CAG-collapse in the mutant allele. Human HD fibroblasts were treated with each TALE-SNP or TALEN. Allele-expression was measured using a SNP-genotyping assay and mutant protein aggregation was quantified with Western blots for anti-ubiquitin. The TALE-SNP and TALEN significantly reduced mutant allele expression (p TALE proteins, and provides a foundation for targeted treatment for individuals suffering from Huntington's or other genetically linked diseases. PMID:26850319

  15. Development of allele-specific multiplex PCR to determine the length of poly-T in intron 8 of CFTR

    Directory of Open Access Journals (Sweden)

    Neng Chen

    2014-07-01

    Full Text Available Cystic fibrosis transmembrane conductance regulator (CFTR gene mutation analysis has been implemented for Cystic Fibrosis (CF carrier screening, and molecular diagnosis of CF and congenital bilateral absence of the vas deferens (CBAVD. Although poly-T allele analysis in intron 8 of CFTR is required when a patient is positive for R117H, it is not recommended for routine carrier screening. Therefore, commercial kits for CFTR mutation analysis were designed either to mask the poly-T allele results, unless a patient is R117H positive, or to have the poly-T analysis as a standalone reflex test using the same commercial platform. There are other standalone assays developed to detect poly-T alleles, such as heteroduplex analysis, High Resolution Melting (HRM curve analysis, allele-specific PCR (AS-PCR and Sanger sequencing. In this report, we developed a simple and easy-to-implement multiplex AS-PCR assay using unlabeled standard length primers, which can be used as a reflex or standalone test for CFTR poly-T track analysis. Out of 115 human gDNA samples tested, results from our new AS-PCR matched to the previous known poly-T results or results from Sanger sequencing.

  16. Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients.

    Science.gov (United States)

    Sata, Hiroshi; Shibayama, Hirohiko; Maeda, Ikuhiro; Habuchi, Yoko; Nakatani, Eiji; Fukushima, Kentaro; Fujita, Jiro; Ezoe, Sachiko; Tadokoro, Seiji; Maeda, Tetsuo; Mizuki, Masao; Kosugi, Satoru; Nakagawa, Masashi; Ueda, Shuji; Iida, Masato; Tokumine, Yukihiro; Azenishi, Yasuhiko; Mitsui, Hideki; Oritani, Kenji; Kanakura, Yuzuru

    2015-05-01

    Quantitative polymerase chain reaction (PCR) with patient-specific, allele-specific oligonucleotide (ASO) primers for individual immunoglobulin H VDJ region (ASO-PCR) amplification was performed using several sources of clinical material, including mRNA from peripheral blood cells (PBMNCs), whole bone marrow cells (BMMNCs), and the CD20+ CD38- B-cell population in bone marrow, as well as cell-free DNA from the sera of patients with multiple myeloma (MM). We designed the ASO primers and produced sufficient PCR fragments to evaluate tumor burden in 20 of 30 bone marrow samples at diagnosis. Polymerase chain reaction amplification efficiency depended on primer sequences because the production of ASO-PCR fragments did not correlate with serum M-protein levels. However, the ASO-PCR levels in BMMNCs showed statistically significant correlations with those in PBMNCs and CD20+ CD38- B-cells. The good association between the BMMNC and PBMNC data indicated that PBMNCs could be a suitable source for monitoring minimal residual disease (MRD). In the case of cell-free DNA, ASO-PCR levels showed a unique pattern and remained high even after treatment. Because the sequence information for each ASO-PCR product was identical to the original, the cell-free DNA might also be useful for evaluating MRD. Moreover, the ASO-PCR products were clearly detected in 17 of 22 mRNA samples from CD20+ CD38- populations, suggesting that MM clones might exist in relatively earlier stages of B cells than in plasma cells. Thus, ASO-PCR analysis using various clinical materials is useful for detecting MRD in MM patients as well as for clarifying MM pathogenesis.

  17. Adoption of Agro-forestry Patterns and Crop Systems Around Register 19 Forest Park, Lampung Province, Indonesia

    Directory of Open Access Journals (Sweden)

    Christine Wulandari

    2014-09-01

    Full Text Available To return the ecological function of Wan Abdul Rachman Forest Park, it must be involved the role of buffer zone communities living around the forest by optimizing the cultivated land with applying agro-forestry based on socio-economic conditions in the community, such as community preferences and adoption of agro-forestry patterns. Under these conditions it is necessary to hold a study concerning to the level of community preference to the type of plants and the level of adoption, as well as the NPV analysis of the 3 patterns of agro-forestry which are applied by the majority of community around the forest park. Results of the study revealed that there were 3 dominant plant types  preferred by the community in the forest park, namely: coffee (30.8%, cacao (35.8%, and rubber (17.4%. Based on these crops, there were 3 agro-forestry patterns practiced by the majority of community in their cultivated land. They were: (1 coffee-cacao-wooden plants, and fruits (47%, (2 rubber-coffee-wooden plants, and fruit (35%, and (3 rubber-cacao-wooden plants, and fruit (18%. The highest personal and social NPVs obtained  in the agro-forestry pattern of rubber-coffee-wooded plants, and fruits were IDR4.589.627.36 and IDR6.454.806.01, respectively. To ensure the sustainability of the program, the development of communities living around the forest together with a program of community empowerment in the block of utilization and social forestry in the forest park are recommended to continue, based on the Regional Regulation (PerDa Number. 3/2012.Keywords: agro-forestry, preferences, adoption, NPV

  18. Integrative analysis of hereditary nonpolyposis colorectal cancer: the contribution of allele-specific expression and other assays to diagnostic algorithms.

    Directory of Open Access Journals (Sweden)

    Laura De Lellis

    Full Text Available The identification of germline variants predisposing to hereditary nonpolyposis colorectal cancer (HNPCC is crucial for clinical management of carriers, but several probands remain negative for such variants or bear variants of uncertain significance (VUS. Here we describe the results of integrative molecular analyses in 132 HNPCC patients providing evidences for improved genetic testing of HNPCC with traditional or next generation methods. Patients were screened for: germline allele-specific expression (ASE, nucleotide variants, rearrangements and promoter methylation of mismatch repair (MMR genes; germline EPCAM rearrangements; tumor microsatellite instability (MSI and immunohistochemical (IHC MMR protein expression. Probands negative for pathogenic variants of MMR genes were screened for germline APC and MUTYH sequence variants. Most germline defects identified were sequence variants and rearrangements of MMR genes. Remarkably, altered germline ASE of MMR genes was detected in 8/22 (36.5% probands analyzed, including 3 cases negative at other screenings. Moreover, ASE provided evidence for the pathogenic role and guided the characterization of a VUS shared by 2 additional probands. No germline MMR gene promoter methylation was observed and only one EPCAM rearrangement was detected. In several cases, tumor IHC and MSI diverged from germline screening results. Notably, APC or biallelic MUTYH germline defects were identified in 2/19 probands negative for pathogenic variants of MMR genes. Our results show that ASE complements gDNA-based analyses in the identification of MMR defects and in the characterization of VUS affecting gene expression, increasing the number of germline alterations detected. An appreciable fraction of probands negative for MMR gene variants harbors APC or MUTYH variants. These results indicate that germline ASE analysis and screening for APC and MUTYH defects should be included in HNPCC diagnostic algorithms.

  19. Oncogene mutations, copy number gains and mutant allele specific imbalance (MASI frequently occur together in tumor cells.

    Directory of Open Access Journals (Sweden)

    Junichi Soh

    Full Text Available BACKGROUND: Activating mutations in one allele of an oncogene (heterozygous mutations are widely believed to be sufficient for tumorigenesis. However, mutant allele specific imbalance (MASI has been observed in tumors and cell lines harboring mutations of oncogenes. METHODOLOGY/PRINCIPAL FINDINGS: We determined 1 mutational status, 2 copy number gains (CNGs and 3 relative ratio between mutant and wild type alleles of KRAS, BRAF, PIK3CA and EGFR genes by direct sequencing and quantitative PCR assay in over 400 human tumors, cell lines, and xenografts of lung, colorectal, and pancreatic cancers. Examination of a public database indicated that homozygous mutations of five oncogenes were frequent (20% in 833 cell lines of 12 tumor types. Our data indicated two major forms of MASI: 1 MASI with CNG, either complete or partial; and 2 MASI without CNG (uniparental disomy; UPD, due to complete loss of wild type allele. MASI was a frequent event in mutant EGFR (75% and was due mainly to CNGs, while MASI, also frequent in mutant KRAS (58%, was mainly due to UPD. Mutant: wild type allelic ratios at the genomic level were precisely maintained after transcription. KRAS mutations or CNGs were significantly associated with increased ras GTPase activity, as measured by ELISA, and the two molecular changes were synergistic. Of 237 lung adenocarcinoma tumors, the small number with both KRAS mutation and CNG were associated with shortened survival. CONCLUSIONS: MASI is frequently present in mutant EGFR and KRAS tumor cells, and is associated with increased mutant allele transcription and gene activity. The frequent finding of mutations, CNGs and MASI occurring together in tumor cells indicates that these three genetic alterations, acting together, may have a greater role in the development or maintenance of the malignant phenotype than any individual alteration.

  20. Infrequent detection of germline allele-specific expression of TGFBR1 in lymphoblasts and tissues of colon cancer patients.

    LENUS (Irish Health Repository)

    Guda, Kishore

    2009-06-15

    Recently, germline allele-specific expression (ASE) of the gene encoding for transforming growth factor-beta type I receptor (TGFBR1) has been proposed to be a major risk factor for cancer predisposition in the colon. Germline ASE results in a lowered expression of one of the TGFBR1 alleles (>1.5-fold), and was shown to occur in approximately 20% of informative familial and sporadic colorectal cancer (CRC) cases. In the present study, using the highly quantitative pyrosequencing technique, we estimated the frequency of ASE in TGFBR1 in a cohort of affected individuals from familial clusters of advanced colon neoplasias (cancers and adenomas with high-grade dysplasia), and also from a cohort of individuals with sporadic CRCs. Cases were considered positive for the presence of ASE if demonstrating an allelic expression ratio <0.67 or >1.5. Using RNA derived from lymphoblastoid cell lines, we find that of 46 informative Caucasian advanced colon neoplasia cases with a family history, only 2 individuals display a modest ASE, with allelic ratios of 1.65 and 1.73, respectively. Given that ASE of TGFBR1, if present, would likely be more pronounced in the colon compared with other tissues, we additionally determined the allele ratios of TGFBR1 in the RNA derived from normal-appearing colonic mucosa of sporadic CRC cases. We, however, found no evidence of ASE in any of 44 informative sporadic cases analyzed. Taken together, we find that germline ASE of TGFBR1, as assayed in lymphoblastoid and colon epithelial cells of colon cancer patients, is a relatively rare event.

  1. Genome-wide identification and quantification of cis- and trans-regulated genes responding to Marek's disease virus infection via analysis of allele-specific expression

    Science.gov (United States)

    Background Marek’s disease (MD) is a commercially important neoplastic disease of chickens caused by the Marek’s disease virus (MDV), a naturally-occurring oncogenic alphaherpesvirus. We attempted to identify genes conferring MD resistance, by completing a genome-wide screen for allele-specific expr...

  2. Adoption of Agro-forestry Patterns and Crop Systems Around Register 19 Forest Park, Lampung Province, Indonesia

    Directory of Open Access Journals (Sweden)

    Christine Wulandari

    2014-08-01

    Full Text Available To return the ecological function of Wan Abdul Rachman Forest Park, it must be involved the role of buffer zone communities living around the forest by optimizing the cultivated land with applying agro-forestry based on socio-economic conditions in the community, such as community preferences and adoption of agro-forestry patterns. Under these conditions it is necessary to hold a study concerning to the level of community preference to the type of plants and the level of adoption, as well as the NPV analysis of the 3 patterns of agro-forestry which are applied by the majority of community around the forest park. Results of the study revealed that there were 3 dominant plant types preferred by the community in the forest park, namely: coffee (30.8%, cacao (35.8%, and rubber (17.4%. Based on these crops, there were 3 agro-forestry patterns practiced by the majority of community in their cultivated land. They were: (1 coffee-cacao-wooden plants, and fruits (47%, (2 rubber-coffee-wooden plants, and fruit (35%, and (3 rubber-cacao-wooden plants, and fruit (18%. The highest personal and social NPVs obtained in the agro-forestry pattern of rubber-coffee-wooded plants, and fruits were IDR4.589.627.36 and IDR6.454.806.01, respectively. To ensure the sustainability of the program, the development of communities living around the forest together with a program of community empowerment in the block of utilization and social forestry in the forest park are recommended to continue, based on the Regional Regulation (PerDa Number. 3/2012.

  3. Impact of pre-existing MSP142-allele specific immunity on potency of an erythrocytic Plasmodium falciparum vaccine

    Directory of Open Access Journals (Sweden)

    Bergmann-Leitner Elke S

    2012-09-01

    Full Text Available Abstract Background MSP1 is the major surface protein on merozoites and a prime candidate for a blood stage malaria vaccine. Preclinical and seroepidemiological studies have implicated antibodies to MSP1 in protection against blood stage parasitaemia and/or reduced parasite densities, respectively. Malaria endemic areas have multiple strains of Plasmodium falciparum circulating at any given time, giving rise to complex immune responses, an issue which is generally not addressed in clinical trials conducted in non-endemic areas. A lack of understanding of the effect of pre-existing immunity to heterologous parasite strains may significantly contribute to vaccine failure in the field. The purpose of this study was to model the effect of pre-existing immunity to MSP142 on the immunogenicity of blood-stage malaria vaccines based on alternative MSP1 alleles. Methods Inbred and outbred mice were immunized with various recombinant P. falciparum MSP142 proteins that represent the two major alleles of MSP142, MAD20 (3D7 and Wellcome (K1, FVO. Humoral immune responses were analysed by ELISA and LuminexTM, and functional activity of induced MSP142-specific antibodies was assessed by growth inhibition assays. T-cell responses were characterized using ex vivo ELISpot assays. Results Analysis of the immune responses induced by various immunization regimens demonstrated a strong allele-specific response at the T cell level in both inbred and outbred mice. The success of heterologous regimens depended on the degree of homology of the N-terminal p33 portion of the MSP142, likely due to the fact that most T cell epitopes reside in this part of the molecule. Analysis of humoral immune responses revealed a marked cross-reactivity between the alleles. Functional analyses showed that some of the heterologous regimens induced antibodies with improved growth inhibitory activities. Conclusion The development of a more broadly efficacious MSP1 based vaccine may be

  4. Allele-specific PCR for detecting the deafness-associated mitochondrial 12S rRNA mutations.

    Science.gov (United States)

    Ding, Yu; Xia, Bo-Hou; Liu, Qi; Li, Mei-Ya; Huang, Shui-Xian; Zhuo, Guang-Chao

    2016-10-10

    Mutations in mitochondrial 12S rRNA (MT-RNR1) are the important causes of sensorineural hearing loss. Of these mutations, the homoplasmic m.1555A>G or m.1494C>T mutation in the highly conserved A-site of MT-RNR1 gene has been found to be associated with both aminoglycoside-induced and non-syndromic hearing loss in many families worldwide. Since the m.1555A>G and m.1494C>T mutations are sensitive to ototoxic drugs, therefore, screening for the presence of these mutations is important for early diagnosis and prevention of deafness. For this purpose, we recently developed a novel allele-specific PCR (AS-PCR) which is able to simultaneously detect these mutations. To assess its accuracy, in this study, we employed this method to screen the frequency of m.1555A>G and m.1494C>T mutations in 200 deafness patients and 120 healthy subjects. Consequently, four m.1555A>G and four m.1494C>T mutations were identified; among these, only one patient with the m.1494C>T mutation had an obvious family history of hearing loss. Strikingly, clinical evaluation showed that this family exhibited a high penetrance of hearing loss. In particular, the penetrances of hearing loss were 80% with the aminoglycoside included and 20% when excluded. PCR-Sanger sequencing of the mitochondrial genomes confirmed the presence of the m.1494C>T mutation and identified a set of polymorphisms belonging to mitochondrial haplogroup A. However, the lack of functional variants in mitochondrial and nuclear modified genes (GJB2 and TRMU) in this family indicated that mitochondrial haplogroup and nuclear genes may not play important roles in the phenotypic expression of the m.1494C>T mutation. Thus, other modification factors, such as environmental factor, aminoglycosides or epigenetic modification may have contributed to the high penetrance of hearing loss in this family. Taken together, our data showed that this assay is an effective approach that could be used for detection the deafness-associated MT-RNR1

  5. WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations

    Directory of Open Access Journals (Sweden)

    Assawamakin Anunchai

    2007-08-01

    Full Text Available Abstract Background Allele-specific (AS Polymerase Chain Reaction is a convenient and inexpensive method for genotyping Single Nucleotide Polymorphisms (SNPs and mutations. It is applied in many recent studies including population genetics, molecular genetics and pharmacogenomics. Using known AS primer design tools to create primers leads to cumbersome process to inexperience users since information about SNP/mutation must be acquired from public databases prior to the design. Furthermore, most of these tools do not offer the mismatch enhancement to designed primers. The available web applications do not provide user-friendly graphical input interface and intuitive visualization of their primer results. Results This work presents a web-based AS primer design application called WASP. This tool can efficiently design AS primers for human SNPs as well as mutations. To assist scientists with collecting necessary information about target polymorphisms, this tool provides a local SNP database containing over 10 million SNPs of various populations from public domain databases, namely NCBI dbSNP, HapMap and JSNP respectively. This database is tightly integrated with the tool so that users can perform the design for existing SNPs without going off the site. To guarantee specificity of AS primers, the proposed system incorporates a primer specificity enhancement technique widely used in experiment protocol. In particular, WASP makes use of different destabilizing effects by introducing one deliberate 'mismatch' at the penultimate (second to last of the 3'-end base of AS primers to improve the resulting AS primers. Furthermore, WASP offers graphical user interface through scalable vector graphic (SVG draw that allow users to select SNPs and graphically visualize designed primers and their conditions. Conclusion WASP offers a tool for designing AS primers for both SNPs and mutations. By integrating the database for known SNPs (using gene ID or rs number

  6. Gene identification and allele-specific marker development for two allelic low phytic acid mutations in rice (Oryza sativa L.)

    International Nuclear Information System (INIS)

    Phytic acid (PA, myo-inositol 1,2,3,4,5,6-hexakisphosphate) is an important anti-nutritional component in cereal and legume grains. PA forms of phosphorus (P) and its salts with micronutrient cations, such as iron and zinc, are indigestible in humans and non-ruminant animals, and hence could affect food/feed nutritional value and cause P pollution of ground water from animal waste. We previously developed a set of low phytic acid (LPA) rice mutants with the aim to increase their nutritional quality. Among them, one line, i.e., Os-lpa -XQZ-1 (hereafter lpa 1-2), was identified to have a mutation allelic to the KBNT lpa 1-1 mutation (hereafter lpa 1-1), which was already delimited to a 47-kb region on chromosome 2. In this study, we searched the candidate gene for these two allelic LPA mutations using T-DNA insertion mutants, mutation detection by CEL I facilitated mismatch cleavage, and gene sequencing. The TIGR locus LOCOs02g57400 was revealed as the candidate gene hosting these two mutations. Sequence analysis showed that the lpa 1-1 is a single base pair substitution mutation, while lpa 1-2 involves a 1,475-bp fragment deletion. A CAPS marker (LPA1CAPS) was developed for distinguishing the lpa 1-1 allele from lpa 1-2 and WT alleles, and InDel marker (LPA1InDel) was developed for differentiating the lpa 1-2 allele from lpa 1-1 and WT ones. Analysis of two populations derived from the two mutants with wild-type varieties confirmed the complete co-segregation of these two markers and LPA phenotype. The LOCOs02g57400 is predicted to encode, through alternative splicing, four possible proteins that are homologous to the 2-phosphoglycerate kinase reported in hyperthermophilic and thermophilic bacteria. The identification of the LPA gene and development of allele-specific markers are of importance not only for breeding LPA varieties, but also for advancing genetics and genomics of phytic acid biosynthesis in rice and other plant species. (author)

  7. Allele-specific PCR for detecting the deafness-associated mitochondrial 12S rRNA mutations.

    Science.gov (United States)

    Ding, Yu; Xia, Bo-Hou; Liu, Qi; Li, Mei-Ya; Huang, Shui-Xian; Zhuo, Guang-Chao

    2016-10-10

    Mutations in mitochondrial 12S rRNA (MT-RNR1) are the important causes of sensorineural hearing loss. Of these mutations, the homoplasmic m.1555A>G or m.1494C>T mutation in the highly conserved A-site of MT-RNR1 gene has been found to be associated with both aminoglycoside-induced and non-syndromic hearing loss in many families worldwide. Since the m.1555A>G and m.1494C>T mutations are sensitive to ototoxic drugs, therefore, screening for the presence of these mutations is important for early diagnosis and prevention of deafness. For this purpose, we recently developed a novel allele-specific PCR (AS-PCR) which is able to simultaneously detect these mutations. To assess its accuracy, in this study, we employed this method to screen the frequency of m.1555A>G and m.1494C>T mutations in 200 deafness patients and 120 healthy subjects. Consequently, four m.1555A>G and four m.1494C>T mutations were identified; among these, only one patient with the m.1494C>T mutation had an obvious family history of hearing loss. Strikingly, clinical evaluation showed that this family exhibited a high penetrance of hearing loss. In particular, the penetrances of hearing loss were 80% with the aminoglycoside included and 20% when excluded. PCR-Sanger sequencing of the mitochondrial genomes confirmed the presence of the m.1494C>T mutation and identified a set of polymorphisms belonging to mitochondrial haplogroup A. However, the lack of functional variants in mitochondrial and nuclear modified genes (GJB2 and TRMU) in this family indicated that mitochondrial haplogroup and nuclear genes may not play important roles in the phenotypic expression of the m.1494C>T mutation. Thus, other modification factors, such as environmental factor, aminoglycosides or epigenetic modification may have contributed to the high penetrance of hearing loss in this family. Taken together, our data showed that this assay is an effective approach that could be used for detection the deafness-associated MT-RNR1

  8. Detection of „Hotspot Mutations in Catalytic Subunit of Phosphatidylinositol 3-Kinase (Pik3ca by Allele-Specific Polymerase Chain Reaction

    Directory of Open Access Journals (Sweden)

    A. Mendelova

    2014-09-01

    Full Text Available The phosphatidylinositol 3-kinases (PI3Ks are a family of proteins involved in the regulation of cell survival, growth, metabolism, and glucose homeostasis. Increased PI3K activity is associated with many cancers. PIK3CA gene (encoding p110 , the catalytic subunit of PI3K is commonly mutated in breast cancer. In our study we focused on the detection of “hotspot” mutations in exons 9 and 20 of the PIK3CA gene in paraffin-embedded tissue of patients with breast cancer. We optimized conditions of allele specific polymerase chain reaction (PCR and we used direct sequencing to verify our results. Overall, three “hotspot” mutations in PIK3CA gene in paraffin-embadded tissue from breast cancer were detected by allele-specific PCR. All results were verified by direct sequencing of PCR products and we observed 100% agreement between those two methods. We confirmed that allele-specific PCR assay is low cost method usefull for accurate detection of PIK3CA mutations.

  9. Clinician Adoption Patterns and Patient Outcome Results in Use of Evidence-Based Nursing Plans of Care

    Science.gov (United States)

    Kim, Tae Youn; Lang, Norma M.; Berg, Karen; Weaver, Charlotte; Murphy, Judy; Ela, Sue

    2007-01-01

    Delivery of safe, effective and appropriate health care is an imperative facing health care organizations globally. While many initiatives have been launched in a number of countries to address this need from a medical perspective, a similar focus for generating evidence-based nursing knowledge has been missing [1]. This paper reports on a collaborative evidence-based practice (EBP) research initiative that adds nursing knowledge into computerized care protocols. Here, a brief overview of the study’s aims, purpose and methodology is presented as well as results of data analysis and lessons learned. The research team examined nurses’ adoption patterns of EBP recommendations with respect to activity tolerance using four-month patient data collected from a pilot hospital. Study findings indicate a need for more focus on the system design and implementation process with the next rollout phase to promote evidence-based nursing practice. PMID:18693871

  10. Development of a Melting Curve-Based Allele-Specific PCR of Apolipoprotein E (APOE) Genotyping Method for Genomic DNA, Guthrie Blood Spot, and Whole Blood.

    Science.gov (United States)

    Chen, Chia-Hsiang

    2016-01-01

    Genetic polymorphisms of apolipoprotein E (APOE) are associated with various health conditions and diseases, such as Alzheimer's disease, cardiovascular diseases, type 2 diabetes, etc. Hence, genotyping of APOE has broad applications in biomedical research and clinical settings, particularly in the era of precision medicine. The study aimed to develop a convenient and accurate method with flexible throughput to genotype the APOE polymorphisms. A melting curve-based allele-specific PCR method was developed to genotype two single nucleotide polymorphisms (SNPs) of APOE, i.e. rs429358 at codon 112 and rs7412 at codon 158. These two SNPs determine the genotype of APOE2, E3, and E4. PCR-based Sanger sequencing was used as the reference method for APOE genotyping. A 100% concordance rate was obtained in 300 subjects between the melting curve-based allele-specific PCR method and the Sanger sequencing method. This method was applied to a genetic association analysis of APOE and schizophrenia consisting of 711 patients with schizophrenia and 665 control subjects from Taiwan. However, no significant differences in the allele and genotype frequencies were detected between these two groups. Further experiments showed that DNA dissolved from blood collected on Guthrie filter paper and total blood cell lysate without DNA extraction can be used in the melting curve-based allele-specific PCR method. Thus, we suggest that this is a fast, accurate and robust APOE genotyping method with a flexible throughput and suitable for DNA template from different preparations. This convenient method shall meet the different needs of various research and clinical laboratories. PMID:27078154

  11. SNP55, a new functional polymorphism of MDM2-P2 promoter, contributes to allele-specific expression of MDM2 in endometrial cancers

    OpenAIRE

    OKAMOTO, KANAKO; Tsunematsu, Ryosuke; Tahira, Tomoko; Sonoda, Kenzo; Asanoma, Kazuo; Yagi, Hiroshi; Yoneda, Tomoko; Hayashi, Kenshi; Wake, Norio; Kato, Kiyoko

    2015-01-01

    Background The functional single nucleotide polymorphism (SNP) in the MDM2 promoter region, SNP309, is known to be associated with various diseases, particularly cancer. Although many studies have been performed to demonstrate the mechanism of allele-specific expression (ASE) on SNP309, they have only utilized in vitro techniques. It is unknown whether ASE of MDM2 is ascribed solely to SNP309, in vivo. Methods We attempted to evaluate ASE of MDM2 in vivo using post-labeling followed by automa...

  12. Allele-specific polymerase chain reaction for detection of a mutation in the relax circular DNA and the covalently closed circular DNA of hepatitis B virus.

    Science.gov (United States)

    Pan, Wan-Long; Hu, Jie-Li; Fang, Yan; Luo, Qiang; Xu, Ge; Xu, Lei; Jing, Zhou-Hong; Shan, Xue-Feng; Zhu, Yan-Ling; Huang, Ai-Long

    2013-12-01

    The relax circle DNA (rcDNA) sequence and the covalently closed circle DNA (cccDNA) sequence in hepatitis B virus (HBV) are crucial regions for HBV infections. To analyze mutations in rcDNA and cccDNA, DNA sequencing is often used, although it is time-consuming and expensive. Herein, we report a simple, economic, albeit accurate allele-specific polymerase chain reaction (AS-PCR) to detect mutations in these regions of HBV. This method can be extensively used to screen for mutations at specific positions of HBV genome.

  13. Allele Specific Locked Nucleic Acid Quantitative PCR (ASLNAqPCR): An Accurate and Cost-Effective Assay to Diagnose and Quantify KRAS and BRAF Mutation

    Science.gov (United States)

    Morandi, Luca; de Biase, Dario; Visani, Michela; Cesari, Valentina; De Maglio, Giovanna; Pizzolitto, Stefano; Pession, Annalisa; Tallini, Giovanni

    2012-01-01

    The use of tyrosine kinase inhibitors (TKIs) requires the testing for hot spot mutations of the molecular effectors downstream the membrane-bound tyrosine kinases since their wild type status is expected for response to TKI therapy. We report a novel assay that we have called Allele Specific Locked Nucleic Acid quantitative PCR (ASLNAqPCR). The assay uses LNA-modified allele specific primers and LNA-modified beacon probes to increase sensitivity, specificity and to accurately quantify mutations. We designed primers specific for codon 12/13 KRAS mutations and BRAF V600E, and validated the assay with 300 routine samples from a variety of sources, including cytology specimens. All were analyzed by ASLNAqPCR and Sanger sequencing. Discordant cases were pyrosequenced. ASLNAqPCR correctly identified BRAF and KRAS mutations in all discordant cases and all had a mutated/wild type DNA ratio below the analytical sensitivity of the Sanger method. ASLNAqPCR was 100% specific with greater accuracy, positive and negative predictive values compared with Sanger sequencing. The analytical sensitivity of ASLNAqPCR is 0.1%, allowing quantification of mutated DNA in small neoplastic cell clones. ASLNAqPCR can be performed in any laboratory with real-time PCR equipment, is very cost-effective and can easily be adapted to detect hot spot mutations in other oncogenes. PMID:22558339

  14. New prediction model for probe specificity in an allele-specific extension reaction for haplotype-specific extraction (HSE) of Y chromosome mixtures.

    Science.gov (United States)

    Rothe, Jessica; Watkins, Norman E; Nagy, Marion

    2012-01-01

    Allele-specific extension reactions (ASERs) use 3' terminus-specific primers for the selective extension of completely annealed matches by polymerase. The ability of the polymerase to extend non-specific 3' terminal mismatches leads to a failure of the reaction, a process that is only partly understood and predictable, and often requires time-consuming assay design. In our studies we investigated haplotype-specific extraction (HSE) for the separation of male DNA mixtures. HSE is an ASER and provides the ability to distinguish between diploid chromosomes from one or more individuals. Here, we show that the success of HSE and allele-specific extension depend strongly on the concentration difference between complete match and 3' terminal mismatch. Using the oligonucleotide-modeling platform Visual Omp, we demonstrated the dependency of the discrimination power of the polymerase on match- and mismatch-target hybridization between different probe lengths. Therefore, the probe specificity in HSE could be predicted by performing a relative comparison of different probe designs with their simulated differences between the duplex concentration of target-probe match and mismatches. We tested this new model for probe design in more than 300 HSE reactions with 137 different probes and obtained an accordance of 88%.

  15. Long-Range (17.7 kb) Allele-Specific Polymerase Chain Reaction Method for Direct Haplotyping of R117H and IVS-8 Mutations of the Cystic Fibrosis Transmembrane Regulator Gene

    OpenAIRE

    Pont-Kingdon, Genevieve; Jama, Mohamed; Miller, Christine; Millson, Alison; Lyon, Elaine

    2004-01-01

    Genotyping of genetic polymorphisms is widely used in clinical molecular laboratories to confirm or predict diseases due to single locus mutations. In contrast, very few molecular methods determine the phase or haplotype of two or more mutations that are kilobases apart. In this report, we describe a new method for haplotyping based on long-range allele-specific PCR. Reaction conditions were established to circumvent the incompatibility of using allele-specific primers and a polymerase with p...

  16. Computer Attitude, and the Impact of Personal Characteristics and Information and Communication Technology Adoption Patterns on Performance of Teaching Faculty in Higher Education in Ghana, West Africa

    Science.gov (United States)

    Larbi-Apau, Josephine A.

    2011-01-01

    This study examined computer attitude, and the impact of personal characteristics and ICT adoption patterns on performance of multidisciplinary teaching faculty in three public universities in Ghana. A cross-sectional research of mixed methods was applied in collecting data and information. Quantitative data from 164 respondents were analyzed…

  17. New technologies adoption and diffusion patterns in developing countries. An empirical study for the period 2000-2011.

    OpenAIRE

    Lechman, Ewa

    2013-01-01

    In recent years, enormous changes are noted worldwide during broad adoption of Information and Communication Technologies (ICT). These unique technologies – often perceived as economic development incentives – have a great ability to spread at high pace and low cost in countries all over the world, bringing people opportunities to contribute to economic development and growth. New Technologies play a special role in developing countries, where their in-country adoption lies in the centre of d...

  18. The Plasmodium falciparum merozoite surface protein-1 19 KD antibody response in the Peruvian Amazon predominantly targets the non-allele specific, shared sites of this antigen

    Directory of Open Access Journals (Sweden)

    Silva Claudia

    2010-01-01

    Full Text Available Abstract Background Plasmodium falciparum re-emerged in Iquitos, Peru in 1994 and is now hypoendemic (P. falciparum infections can be followed using this population dynamic. Previous work demonstrated a strong association between this population's antibody response to PfMSP1-19KD and protection against febrile illness and parasitaemia. Therefore, some selection for PfMSP1-19KD allelic diversity would be expected if the protection is to allele-specific sites of PfMSP1-19KD. Here, the potential for allele-specific polymorphisms in this population is investigated, and the allele-specificity of antibody responses to PfMSP1-19KD are determined. Methods The 42KD region in PfMSP1 was genotyped from 160 individual infections collected between 2003 and 2007. Additionally, the polymorphic block 2 region of Pfmsp1 (Pfmsp1-B2 was genotyped in 781 infection-months to provide a baseline for population-level diversity. To test whether PfMSP1-19KD genetic diversity had any impact on antibody responses, ELISAs testing IgG antibody response were performed on individuals using all four allele-types of PfMSP1-19KD. An antibody depletion ELISA was used to test the ability of antibodies to cross-react between allele-types. Results Despite increased diversity in Pfmsp1-B2, limited diversity within Pfmsp1-42KD was observed. All 160 infections genotyped were Mad20-like at the Pfmsp1-33KD locus. In the Pfmsp1-19KD locus, 159 (99.4% were the Q-KSNG-F haplotype and 1 (0.6% was the E-KSNG-L haplotype. Antibody responses in 105 individuals showed that Q-KNG and Q-TSR alleles generated the strongest immune responses, while Q-KNG and E-KNG responses were more concordant with each other than with those from Q-TSR and E-TSR, and vice versa. The immuno-depletion ELISAs showed all samples responded to the antigenic sites shared amongst all allelic forms of PfMSP1-19KD. Conclusions A non-allele specific antibody response in PfMSP1-19KD may explain why other allelic forms have not

  19. Differences in influence patterns between groups predicting the adoption of a solar disinfection technology for drinking water in Bolivia.

    Science.gov (United States)

    Moser, Stephanie; Mosler, Hans-Joachim

    2008-08-01

    The lack of safe drinking water is one of the major problems faced by developing countries. The consequences of contaminated water are diseases such as diarrhea, one of the main causes of infant mortality. Because of its simplicity, solar water-disinfection technology provides a good way of treating water at the household level. Despite its obvious advantages and considerable promotional activities, this innovation has had rather a slow uptake. We conducted a field survey in which 644 households in Bolivia were interviewed in order to gain insights on motivations that resulted in adopting the technology. The aim was to examine possible differences in the predictors for adopting this technology during the diffusion process using the theory of innovation diffusion. Our findings indicate that early adoption was predicted by increased involvement in the topic of drinking water and that adoption in the middle of the diffusion process was predicted by increased involvement by opinion leaders and by recognition of a majority who supported the technology. Finally, late adoption was predicted by recognition that a majority had already adopted. Suggestions for future promotional strategies are outlined. PMID:18508169

  20. Allele-specific real-time PCR testing for minor HIV-1 drug resistance mutations: assay preparation and application to reveal dynamic of mutations in vivo

    Institute of Scientific and Technical Information of China (English)

    GUO Dong-xing; LI Jing-yun; LI Han-ping; LI Lin; ZHUANG Dao-min; JIAO Li-yan; WANG Zheng; BAO Zuo-yi; LIU Si-yang; LIU Yong-jian

    2010-01-01

    Background It is very important for the clinical management to test for minor HIV-1 resistance mutations accurately and sensitively. The conventional genotypic assays of HIV drug resistance detection based on sequencing can only discriminate the mutations which present in more than 20%-30%. The aim of this study was to evaluate allele-specific real-time PCR (ASPCR) to detect the resistance-related mutations located at positions 103, 184 and 215.Methods We developed the allele-specific PCR assay, using the most common drug resistance mutations in Chinese AIDS patients, K103N, M184V/I, T215F/Y as a model system. The standards were constructed by cloning the wild-type and mutant DNA fragments into the T-vector. We designed specific primers to discriminate mutant templates in the real-time PCR using SYBR green as a fluorescence reporter. And then we evaluated the ASPCR assay and tested 140clinical samples using this method.Results The sensitivities of ASPCR assay were 0.04% for K103N, 0.30% for M1841, 0.40% for M184V, 0.03% for T215F and 0.02% for T215Y. The intra-assay and inter-assay coefficients of variation were less than 0.42. One hundred and forty plasma samples were tested by ASPCR and dynamic resistance curves of ten patients were obtained.Conclusions Drug resistance emerged half a year after the start of antiretroviral therapy. The mutation of T215Yemerged 1 to 1.5 years after starting treatment and then increased rapidly. The ASPCR assay we developed was a sensitive, accurate and rapid method to detect the minor HIV-1 variants and it can provide earlier and more drug-resistance information for HIV research and AIDS antiretroviral therapy.

  1. Multiplex genotyping of KRAS point mutations in tumor cell DNA by allele-specific real-time PCR on a centrifugal microfluidic disk segment

    International Nuclear Information System (INIS)

    Point Mutations on the Kirsten rat sarcoma viral oncogene homolog (KRAS) have been identified as an important predictive biomarker for response to cancer therapy targeting the epidermal growth factor receptor. KRAS mutations are prevalent in up to 40 % of all colorectal carcinomas, and routinely conducted KRAS genotyping is becoming mandatory to predict therapy success and to reduce therapy costs. We report a low-cost, disposable and ready-to-use centrifugal microfluidic cartridge (termed GeneSlice) containing preloaded primers and probes. The GeneSlice cartridge enables the parallel detection of the seven most relevant KRAS point mutations by allele-specific real-time PCR. It represents a cost effective alternative to dideoxy-sequencing with a faster time-to-result (∼ 2 h versus up to 20 h in case of dd-sequencing). Microfluidic processing of the GeneSlice along with allele-specific amplification and real-time detection are conducted in a slightly modified, commercially available PCR thermocycler. Intra-chip standard deviation of Cq values on the GeneSlices is negligible (GeneSlice 1: Cq,std.dev. = 0.13; GeneSlice 2: Cq,std.dev = 0.26). In 23 of 24 experiments, the data for genotyping 6 cancer cell lines (n = 4 per cell line) agreed with dd-sequencing. Additionally, DNA derived from microdissected formalin-fixed and paraffin embedded colorectal carcinomas of two cases was genotyped correctly and reproducibly (n = 3 per patient; one GeneSlice excluded from evaluation). The GeneSlice therefore clearly demonstrated the potential to become a valuable tool for routine diagnostics of KRAS mutations by reducing costs and hands-on time. (author)

  2. Disagreement in genotyping results of drug resistance alleles of the Plasmodium falciparum dihydrofolate reductase (Pfdhfr) gene by allele-specific PCR (ASPCR) assays and Sanger sequencing.

    Science.gov (United States)

    Sharma, Divya; Lather, Manila; Dykes, Cherry L; Dang, Amita S; Adak, Tridibes; Singh, Om P

    2016-01-01

    The rapid spread of antimalarial drug resistance in Plasmodium falciparum over the past few decades has necessitated intensive monitoring of such resistance for an effective malaria control strategy. P. falciparum dihydropteroate synthase (Pfdhps) and P. falciparum dihydrofolate reductase (Pfdhfr) genes act as molecular markers for resistance against the antimalarial drugs sulphadoxine and pyrimethamine, respectively. Resistance to pyrimethamine which is used as a partner drug in artemisinin combination therapy (ACT) is associated with several mutations in the Pfdhfr gene, namely A16V, N51I, C59R, S108N/T and I164L. Therefore, routine monitoring of Pfdhfr-drug-resistant alleles in a population may help in effective drug resistance management. Allele-specific PCR (ASPCR) is one of the commonly used methods for molecular genotyping of these alleles. In this study, we genotyped 55 samples of P. falciparum for allele discrimination at four codons of Pfdhfr (N51, C59, S108 and I164) by ASPCR using published methods and by Sanger's DNA sequencing method. We found that the ASPCR identified a significantly higher number of mutant alleles as compared to the DNA sequencing method. Such discrepancies arise due to the non-specificity of some of the allele-specific primer sets and due to the lack of sensitivity of Sanger's DNA sequencing method to detect minor alleles present in multiple clone infections. This study reveals the need of a highly specific and sensitive method for genotyping and detecting minor drug-resistant alleles present in multiple clonal infections.

  3. Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi.

    Directory of Open Access Journals (Sweden)

    Yusuke Ohnishi

    Full Text Available Allele-specific gene silencing by RNA interference (RNAi is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi, the design and assessment of small interfering RNA (siRNA duplexes conferring ASP-RNAi is vital; however, it is also difficult. In a previous study, we developed an assay system to assess ASP-RNAi with mutant and wild-type reporter alleles encoding the Photinus and Renilla luciferase genes. In line with experiments using the system, we realized that it is necessary and important to enhance allele discrimination between mutant and corresponding wild-type alleles. Here, we describe the improvement of ASP-RNAi against mutant alleles carrying single nucleotide variations by introducing base substitutions into siRNA sequences, where original variations are present in the central position. Artificially mismatched siRNAs or short-hairpin RNAs (shRNAs against mutant alleles of the human Prion Protein (PRNP gene, which appear to be associated with susceptibility to prion diseases, were examined using this assessment system. The data indicates that introduction of a one-base mismatch into the siRNAs and shRNAs was able to enhance discrimination between the mutant and wild-type alleles. Interestingly, the introduced mismatches that conferred marked improvement in ASP-RNAi, appeared to be largely present in the guide siRNA elements, corresponding to the 'seed region' of microRNAs. Due to the essential role of the 'seed region' of microRNAs in their association with target RNAs, it is conceivable that disruption of the base-pairing interactions in the corresponding seed region, as well as the central position (involved in cleavage of target RNAs, of guide siRNA elements could influence allele discrimination. In addition, we also suggest that nucleotide mismatches at the 3'-ends of sense

  4. Concordance between allele-specific PCR and ultra-deep pyrosequencing for the detection of HIV-1 non-nucleoside reverse transcriptase inhibitor resistance mutations

    Science.gov (United States)

    Hunt, Gillian M; Morris, Lynn; Moorthy, Anitha; Coovadia, Ashraf; Abrams, Elaine J; Strehlau, Renate; Kuhn, Louise; Persaud, Deborah

    2014-01-01

    Recent advances in genotyping technologies have allowed for detection of HIV-1 drug resistance mutations present at low levels. The presence and percentage of Y181C and K103N drug-resistant variants in the blood of 105 subtype C HIV-infected infants who failed single-dose nevirapine prophylaxis for HIV transmission were compared using two highly sensitive genotyping methods, allele-specific PCR (AS-PCR) and ultra-deep pyrosequencing. Significant correlations in detection between both methods were found for both Y181C (correlation coefficients of 0.94 [95% CI 0.91-0.96]) and K103N (0.89 [95% CI 0.84 – 0.92]) mutations. The majority of discordant specimens (3/5 Y181C and 8/11 K103N) had wild-type variants when population sequencing was used, but mutant variants were detectable at very low levels (≤5%) with either assay. This difference is most likely due to stochastic variations in the appearance of mutant variants. Overall, both AS-PCR and ultra-deep pyrosequencing methods have proven to be sensitive and accurate, and may confidently be used where feasible. PMID:25034127

  5. A Multiplex Allele Specific Polymerase Chain Reaction (MAS-PCR) for the Detection of Factor V Leiden and Prothrombin G20210A

    Science.gov (United States)

    Bagheri, Morteza; Rad, Isa Abdi

    2011-01-01

    ABSTRACT Introduction: In order to determine the frequencies of factor V Leiden and prothrombin G20210A point mutations in the Iranian population with Azeri Turkish origin. Material and methods: 120 unrelated individuals from general population randomly selected and were examined for factor V Leiden and prothrombin G20210A mutations using a multiplex allele specific polymerase chain reaction (MAS-PCR) assay Outcomes: The frequency of prothrombin G20210A mutation was 2.08%, which means 5 chromosomes out of 240 chromosomes had prothrombin G20210A mutation. The distribution of prothrombin 20210 GG, GA, AA genotypes and prothrombin 20210A allele were 37(92.5%), 3(7.5%), 0(0%) and 3(3.75%) in males and 78(97.5%), 2(2.5%), 0(0%) and 2(1.25%) in females, respectively. Factor V Leiden was not found in our tested group (zero chromosomes out of 240 chromosomes). Analysis of the observed frequencies in the studied groups indicates that there is no statistically significant difference between females and males, regarding prothrombin G20210A mutation (p value>0.05). Conclusions: This is the first study in its own kind in this population and implies that the frequency of Factor V Leiden G1691A (R506Q, FV-Leiden) allele is extremely low but the prothrombin G20210A mutation is more frequent in the tested group. PMID:21977183

  6. Evaluation of a blood-specific DNA methylated region and trial for allele-specific blood identification from mixed body fluid DNA.

    Science.gov (United States)

    Watanabe, Ken; Akutsu, Tomoko; Takamura, Ayari; Sakurada, Koichi

    2016-09-01

    The identification of blood samples obtained from crime scenes has been an important step in forensic investigation. Recently, a novel approach using the blood-specific methylated CpG site cg06379435 has been reported. In this study, we developed a real-time polymerase-chain-reaction-based method that can simply and rapidly quantitate the methylation ratio of cg06379435 and its neighboring CpGs and set the threshold ratios for blood identification by analyzing various body fluid samples. Blood identification using the thresholds was successfully performed in the analysis of a small amount (1ng) of DNA from blood and various aged blood samples, including 29-year-old stains. We also demonstrated a test for allele-specific blood identification from a mixed DNA sample by bisulfite sequencing analysis of these CpG sites and their neighboring single nucleotide polymorphism, rs7359943 (A/G), which is of relevance in cases where mixed samples are obtained from crime scenes. The stability of DNA methylation in aged samples and the usefulness of neighboring genetic information shown in this study suggest that DNA-methylation-based body fluid identification will play a major role in future forensic investigations. PMID:27591539

  7. Rapid KRAS, EGFR, BRAF and PIK3CA Mutation Analysis of Fine Needle Aspirates from Non-Small-Cell Lung Cancer Using Allele-Specific qPCR

    Science.gov (United States)

    Schrumpf, Melanie; Talebian Yazdi, Mehrdad; Ruano, Dina; Forte, Giusi I.; Nederlof, Petra M.; Veselic, Maud; Rabe, Klaus F.; Annema, Jouke T.; Smit, Vincent; Morreau, Hans; van Wezel, Tom

    2011-01-01

    Endobronchial Ultrasound Guided Transbronchial Needle Aspiration (EBUS-TBNA) and Trans-esophageal Ultrasound Scanning with Fine Needle Aspiration (EUS-FNA) are important, novel techniques for the diagnosis and staging of non-small cell lung cancer (NSCLC) that have been incorporated into lung cancer staging guidelines. To guide and optimize treatment decisions, especially for NSCLC patients in stage III and IV, EGFR and KRAS mutation status is often required. The concordance rate of the mutation analysis between these cytological aspirates and histological samples obtained by surgical staging is unknown. Therefore, we studied the extent to which allele-specific quantitative real-time PCR with hydrolysis probes could be reliably performed on EBUS and EUS fine needle aspirates by comparing the results with histological material from the same patient. We analyzed a series of 43 NSCLC patients for whom cytological and histological material was available. We demonstrated that these standard molecular techniques can be accurately applied on fine needle cytological aspirates from NSCLC patients. Importantly, we show that all mutations detected in the histological material of primary tumor were also identified in the cytological samples. We conclude that molecular profiling can be reliably performed on fine needle cytology aspirates from NSCLC patients. PMID:21408138

  8. Rapid KRAS, EGFR, BRAF and PIK3CA mutation analysis of fine needle aspirates from non-small-cell lung cancer using allele-specific qPCR.

    Directory of Open Access Journals (Sweden)

    Ronald van Eijk

    Full Text Available Endobronchial Ultrasound Guided Transbronchial Needle Aspiration (EBUS-TBNA and Trans-esophageal Ultrasound Scanning with Fine Needle Aspiration (EUS-FNA are important, novel techniques for the diagnosis and staging of non-small cell lung cancer (NSCLC that have been incorporated into lung cancer staging guidelines. To guide and optimize treatment decisions, especially for NSCLC patients in stage III and IV, EGFR and KRAS mutation status is often required. The concordance rate of the mutation analysis between these cytological aspirates and histological samples obtained by surgical staging is unknown. Therefore, we studied the extent to which allele-specific quantitative real-time PCR with hydrolysis probes could be reliably performed on EBUS and EUS fine needle aspirates by comparing the results with histological material from the same patient. We analyzed a series of 43 NSCLC patients for whom cytological and histological material was available. We demonstrated that these standard molecular techniques can be accurately applied on fine needle cytological aspirates from NSCLC patients. Importantly, we show that all mutations detected in the histological material of primary tumor were also identified in the cytological samples. We conclude that molecular profiling can be reliably performed on fine needle cytology aspirates from NSCLC patients.

  9. A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection.

    Science.gov (United States)

    Zhang, X J; Wang, L X; Chen, X X; Liu, Y L; Meng, R; Wang, Y J; Zhao, Z Y

    2014-01-01

    Pre-selection for fruit skin color at the seedling stage would be highly advantageous, with marker-assisted selection offering a potential method for apple pre-selection. A and MdMYB1 alleles are allele-specific DNA markers that are potentially associated with apple skin color, and co-segregate with the Rf and Rni loci, respectively. Here, we assessed the potential application of these 2 alleles for marker-assisted breeding across 30 diverse cultivars and 2 apple seedling progenies. The red skin color phenotype was usually associated with the MdMYB1-1 allele and A(1) allele, respectively, while the 2 molecular markers provided approximately 91% predictability in the 'Fuji' x 'Cripps Pink' and 'Fuji' x 'Gala' progenies. The results obtained from the 30 cultivars and 2 progenies were consistent for the 2 molecular markers. Hence, the results supported that Rf and Rni could be located in a gene cluster, or even correspond to alleles of the same gene. Our results are consistent with the hypothesis that red/yellow dimorphism is controlled by a monogenic system, with the presence of the red anthocyanin pigmentation being dominant. In addition, our results supported that the practical utilization of the 2 function markers to efficiently and accurately select red-skinned apple cultivars in apple scion breeding programs.

  10. Allele-specific virulence attenuation of the Pseudomonas syringae HopZ1a type III effector via the Arabidopsis ZAR1 resistance protein.

    Directory of Open Access Journals (Sweden)

    Jennifer D Lewis

    2010-04-01

    Full Text Available Plant resistance (R proteins provide a robust surveillance system to defend against potential pathogens. Despite their importance in plant innate immunity, relatively few of the approximately 170 R proteins in Arabidopsis have well-characterized resistance specificity. In order to identify the R protein responsible for recognition of the Pseudomonas syringae type III secreted effector (T3SE HopZ1a, we assembled an Arabidopsis R gene T-DNA Insertion Collection (ARTIC from publicly available Arabidopsis thaliana insertion lines and screened it for plants lacking HopZ1a-induced immunity. This reverse genetic screen revealed that the Arabidopsis R protein HOPZ-activated resistance 1 (ZAR1; At3g50950 is required for recognition of HopZ1a in Arabidopsis. ZAR1 belongs to the coiled-coil (CC class of nucleotide binding site and leucine-rich repeat (NBS-LRR containing R proteins; however, the ZAR1 CC domain phylogenetically clusters in a clade distinct from other related Arabidopsis R proteins. ZAR1-mediated immunity is independent of several genes required by other R protein signaling pathways, including NDR1 and RAR1, suggesting that ZAR1 possesses distinct signaling requirements. The closely-related T3SE protein, HopZ1b, is still recognized by zar1 Arabidopsis plants indicating that Arabidopsis has evolved at least two independent R proteins to recognize the HopZ T3SE family. Also, in Arabidopsis zar1 plants HopZ1a promotes P. syringae growth indicative of an ancestral virulence function for this T3SE prior to the evolution of recognition by the host resistance protein ZAR1. Our results demonstrate that the Arabidopsis resistance protein ZAR1 confers allele-specific recognition and virulence attenuation of the Pseudomonas syringae T3SE protein HopZ1a.

  11. Correction of Mutant p63 in EEC Syndrome Using siRNA Mediated Allele-Specific Silencing Restores Defective Stem Cell Function.

    Science.gov (United States)

    Barbaro, Vanessa; Nasti, Annamaria A; Del Vecchio, Claudia; Ferrari, Stefano; Migliorati, Angelo; Raffa, Paolo; Lariccia, Vincenzo; Nespeca, Patrizia; Biasolo, Mariangela; Willoughby, Colin E; Ponzin, Diego; Palù, Giorgio; Parolin, Cristina; Di Iorio, Enzo

    2016-06-01

    Ectrodactyly-Ectodermal dysplasia-Clefting (EEC) syndrome is a rare autosomal dominant disease caused by heterozygous mutations in the p63 gene and characterized by limb defects, orofacial clefting, ectodermal dysplasia, and ocular defects. Patients develop progressive total bilateral limbal stem cell deficiency, which eventually results in corneal blindness. Medical and surgical treatments are ineffective and of limited benefit. Oral mucosa epithelial stem cells (OMESCs) represent an alternative source of stem cells capable of regenerating the corneal epithelium and, combined with gene therapy, could provide an attractive therapeutic avenue. OMESCs from EEC patients carrying the most severe p63 mutations (p.R279H and p.R304Q) were characterized and the genetic defect of p.R279H silenced using allele-specific (AS) small interfering RNAs (siRNAs). Systematic screening of locked nucleic acid (LNA)-siRNAs against R279H-p63 allele in (i) stable WT-ΔNp63α-RFP and R279H-ΔNp63α-EGFP cell lines, (ii) transient doubly transfected cell lines, and (iii) p.R279H OMESCs, identified a number of potent siRNA inhibitors for the mutant allele, which had no effect on wild-type p63. In addition, siRNA treatment led to longer acquired life span of mutated stem cells compared to controls, less accelerated stem cell differentiation in vitro, reduced proliferation properties, and effective ability in correcting the epithelial hypoplasia, thus giving rise to full thickness stratified and differentiated epithelia. This study demonstrates the phenotypic correction of mutant stem cells (OMESCs) in EEC syndrome by means of siRNA mediated AS silencing with restoration of function. The application of siRNA, alone or in combination with cell-based therapies, offers a therapeutic strategy for corneal blindness in EEC syndrome. Stem Cells 2016;34:1588-1600. PMID:26891374

  12. SAAS-CNV: A Joint Segmentation Approach on Aggregated and Allele Specific Signals for the Identification of Somatic Copy Number Alterations with Next-Generation Sequencing Data.

    Directory of Open Access Journals (Sweden)

    Zhongyang Zhang

    2015-11-01

    Full Text Available Cancer genomes exhibit profound somatic copy number alterations (SCNAs. Studying tumor SCNAs using massively parallel sequencing provides unprecedented resolution and meanwhile gives rise to new challenges in data analysis, complicated by tumor aneuploidy and heterogeneity as well as normal cell contamination. While the majority of read depth based methods utilize total sequencing depth alone for SCNA inference, the allele specific signals are undervalued. We proposed a joint segmentation and inference approach using both signals to meet some of the challenges. Our method consists of four major steps: 1 extracting read depth supporting reference and alternative alleles at each SNP/Indel locus and comparing the total read depth and alternative allele proportion between tumor and matched normal sample; 2 performing joint segmentation on the two signal dimensions; 3 correcting the copy number baseline from which the SCNA state is determined; 4 calling SCNA state for each segment based on both signal dimensions. The method is applicable to whole exome/genome sequencing (WES/WGS as well as SNP array data in a tumor-control study. We applied the method to a dataset containing no SCNAs to test the specificity, created by pairing sequencing replicates of a single HapMap sample as normal/tumor pairs, as well as a large-scale WGS dataset consisting of 88 liver tumors along with adjacent normal tissues. Compared with representative methods, our method demonstrated improved accuracy, scalability to large cancer studies, capability in handling both sequencing and SNP array data, and the potential to improve the estimation of tumor ploidy and purity.

  13. SAAS-CNV: A Joint Segmentation Approach on Aggregated and Allele Specific Signals for the Identification of Somatic Copy Number Alterations with Next-Generation Sequencing Data.

    Science.gov (United States)

    Zhang, Zhongyang; Hao, Ke

    2015-11-01

    Cancer genomes exhibit profound somatic copy number alterations (SCNAs). Studying tumor SCNAs using massively parallel sequencing provides unprecedented resolution and meanwhile gives rise to new challenges in data analysis, complicated by tumor aneuploidy and heterogeneity as well as normal cell contamination. While the majority of read depth based methods utilize total sequencing depth alone for SCNA inference, the allele specific signals are undervalued. We proposed a joint segmentation and inference approach using both signals to meet some of the challenges. Our method consists of four major steps: 1) extracting read depth supporting reference and alternative alleles at each SNP/Indel locus and comparing the total read depth and alternative allele proportion between tumor and matched normal sample; 2) performing joint segmentation on the two signal dimensions; 3) correcting the copy number baseline from which the SCNA state is determined; 4) calling SCNA state for each segment based on both signal dimensions. The method is applicable to whole exome/genome sequencing (WES/WGS) as well as SNP array data in a tumor-control study. We applied the method to a dataset containing no SCNAs to test the specificity, created by pairing sequencing replicates of a single HapMap sample as normal/tumor pairs, as well as a large-scale WGS dataset consisting of 88 liver tumors along with adjacent normal tissues. Compared with representative methods, our method demonstrated improved accuracy, scalability to large cancer studies, capability in handling both sequencing and SNP array data, and the potential to improve the estimation of tumor ploidy and purity.

  14. Minority drug-resistant HIV-1 variants in treatment naive East-African and Caucasian patients detected by allele-specific real-time PCR.

    Directory of Open Access Journals (Sweden)

    Halime Ekici

    Full Text Available To assess the presence of two major non-nucleoside reverse transcriptase inhibitors (NNRTI drug resistance mutations (DRMs, Y181C and K103N, in minor viral quasispecies of treatment naïve HIV-1 infected East-African and Swedish patients by allele-specific polymerase chain reaction (AS-PCR.Treatment naïve adults (n=191 with three epidemiological backgrounds were included: 92 Ethiopians living in Ethiopia; 55 East-Africans who had migrated to Sweden; and 44 Caucasians living in Sweden. The pol gene was analysed by standard population sequencing and by AS-PCR for the detection of Y181C and K103N.The Y181C was detected in the minority quasispecies of six Ethiopians (6.5%, in two Caucasians (4.5%, and in one East-African (1.8%. The K103N was detected in one East- African (1.8%, by both methods. The proportion of mutants ranged from 0.25% to 17.5%. Additional DRMs were found in all three treatment naïve patient groups by population sequencing.Major NNRTI mutations can be found by AS-PCR in minor quasispecies of treatment naïve HIV-1 infected Ethiopians living in Ethiopia, in East-African and Caucasian patients living in Sweden in whom population sequencing reveal wild-type virus only. Surveys with standard sequencing are likely to underestimate transmitted drug resistance and the presence of resistant minor quasispecies in treatment naïve patients should be topic for future large scale studies.

  15. Adoption of M-Commerce

    OpenAIRE

    Saljoughi, Farhad

    2002-01-01

    The development of ICT in recent years, and speed of this development is extremely rapid. ICT also spreads widely and influences deeply not only economic activities of business, households and governments but also various areas of people’s daily life. The adoption rate of mobile Internet was estimated by analogy to adoption pattern. This study examines patterns of mobile commerce adoption and usage in early adopters. One hundred and ninety mobile users were surveyed about their...

  16. Comparison of 454 Ultra-Deep Sequencing and Allele-Specific Real-Time PCR with Regard to the Detection of Emerging Drug-Resistant Minor HIV-1 Variants after Antiretroviral Prophylaxis for Vertical Transmission.

    Directory of Open Access Journals (Sweden)

    Andrea Hauser

    Full Text Available Pregnant HIV-infected women were screened for the development of HIV-1 drug resistance after implementation of a triple-antiretroviral transmission prophylaxis as recommended by the WHO in 2006. The study offered the opportunity to compare amplicon-based 454 ultra-deep sequencing (UDS and allele-specific real-time PCR (ASPCR for the detection of drug-resistant minor variants in the HIV-1 reverse transcriptase (RT.Plasma samples from 34 Tanzanian women were previously analysed by ASPCR for key resistance mutations in the viral RT selected by AZT, 3TC, and NVP (K70R, K103N, Y181C, M184V, T215Y/F. In this study, the RT region of the same samples was investigated by amplicon-based UDS for resistance mutations using the 454 GS FLX System.Drug-resistant HIV-variants were identified in 69% (20/29 of women by UDS and in 45% (13/29 by ASPCR. The absolute number of resistance mutations identified by UDS was twice that identified by ASPCR (45 vs 24. By UDS 14 of 24 ASPCR-detected resistance mutations were identified at the same position. The overall concordance between UDS and ASPCR was 61.0% (25/41. The proportions of variants quantified by UDS were approximately 2-3 times lower than by ASPCR. Amplicon generation from samples with viral loads below 20,000 copies/ml failed more frequently by UDS compared to ASPCR (limit of detection = 650 copies/ml, resulting in missing or insufficient sequence coverage.Both methods can provide useful information about drug-resistant minor HIV-1 variants. ASPCR has a higher sensitivity than UDS, but is restricted to single resistance mutations. In contrast, UDS is limited by its requirement for high viral loads to achieve sufficient sequence coverage, but the sequence information reveals the complete resistance patterns within the genomic region analysed. Improvements to the UDS limit of detection are in progress, and UDS could then facilitate monitoring of drug-resistant minor variants in the HIV-1 quasispecies.

  17. Intercountry adoption

    OpenAIRE

    International Child Development Centre

    2007-01-01

    This Digest looks at intercountry adoption as one of a series of possible solutions for children unable to live with their families. Broadly accepted international instruments specify the conditions under which intercountry adoption should be undertaken if the rights and best interests of the children concerned are to be protected and fully respected. Although substantial efforts are being made to implement the standards and procedures set, current practices are often in violation of these no...

  18. 全血AS-PCR方法检测Wilson病ATP7B基因四个突变%Whole blood allele-specific PCR, a simple method to detect four ATP7B gene mutations in Wilson disease

    Institute of Scientific and Technical Information of China (English)

    孙玮; 管俊杰; 王进; 秦正红

    2014-01-01

    Objective To establish a simple method to detect four ATP7B gene mutations in Wilson disease using allele-specific PCR (AS-PCR) with whole blood polymerase chain reaction.Methods Four allele-specific PCR primers specific for the mutations(G2333T,C2850T,G2855A,G2975T) were designed,and PCR was optimized to screen the whole blood samples.The amplified gene products with mutation were separated with agarose gel electrophoresis to detect the pattern of point mutation and allele types.Exons 8,12 and 13 of the ATP7B gene were amplified with PCR,and the amplification products were sequenced to confirm the mutation.Results The detection of four ATP7B gene mutations by AS-PCR with whole blood was accomplished with 100% accuracy.In the 27 healthy subjects,the mutation rate of G2855A was 51.8%.No mutation was detected for G2333T,C2850T and G2975T.Among the 22 patients,11 were mutated for G2333T,C2850T or G2975T.The mutation rate was therefore 50%.Conclusion Our experiment has established an AS-PCR based method for detecting four ATP7B gene mutations using whole blood samples,which has provided a simple and effective means for the early diagnosis of Wilson disease.This method is rapid,convenient,accurate and economical for detecting point mutations of the ATP7B gene.%目的 研究肝豆状核变性ATP7B基因高频突变位点,探索全血等位基因特异性-PCR(allelespecific PCR,AS-PCR)技术在该基因4个常见突变检测中的应用.方法 针对ATP7B基因4个突变位点(G2333T、C2850T、G2855A、G2975T)设计等位基因特异性引物,应用高保真酶对人抗凝全血样本进行聚合酶链反应,扩增产物经琼脂糖凝胶电泳以判断待检样本有无基因突变及其等位基因型.PCR扩增人基因组A TP7B基因第8、12、13外显子,扩增产物直接进行基因序列测定.结果 全血AS-PCR法检测ATP7B基因4个基因突变,各位点检测结果与基因序列测定完全相符.27份健康对照血样分型结果G2333T、C2850T

  19. DETEKSI MUTASI V1016G PADA GEN VOLTAGE-GATED SODIUM CHANNEL PADA POPULASI Aedes aegypti (DIPTERA: CULICIDAE DI KABUPATEN KLATEN, JAWA TENGAH DENGAN METODE ALLELE-SPECIFIC PCR

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    Dyah Widiastuti

    2015-10-01

    Full Text Available AbstrakMeluasnya kejadian resistensi pada vektor virus Dengue di Jawa Tengah memerlukan strategi pengelolaan resistensi insektisida secara efektif. Oleh karena itu, informasi mengenai mutasi gen pada posisi 1016 di domain II segmen ke­6 gen VGSC pada nyamuk Aedes aegypti yang menyebabkan perubahan asam amino valin (V menjadi glisin (G akan dapat memperkuat penelitian operasional mengenai strategi pemilihan insektisida dalam program­pengendalian­vektor­Dengue.­Penelitian­ini­menggunakan­uji­Allele-Specific­Polymerase­Chain­Reaction(AS­PCR yang dapat mendeteksi mutasi V1016G. Sampel penelitian ini adalah 22 ekor nyamuk Aedes aegypti dari Kabupaten Klaten yang berumur 2­5 hari. Hasil penelitian menunjukkan bahwa 22,7% nyamuk belum mengalami mutasi (V/V, 59,1% nyamuk mengalami mutasi heterozigot (V/G dan 18,2% nyamuk mengalami mutasi homozigot (G/G. Hal ini menunjukkan indikasi terjadinya resistensi populasi nyamuk Ae.aegypti terhadap insektisida sintetik piretroid yang disebabkan oleh mekanisme knockdown resistance.Kata Kunci:­Aedes­aegypti,­mutasi­V1016G,­Allele-Specific­PCR,­VGSCAbstractInsecticides resistance has spread rapidly among dengue vectors from Central Java, and require an effective insecticide resistance management strategies.one of the resistance mechanism in Aedes aegypti may arise through knockdown resistance or kdr which consists of single point mutation within the genes that are targeted by insecticide compounds. Mutation at position 1016 in domain II, segment 6 of the Voltage Gated Sodium Channel gene in Ae. aegypti leads to a valine to glycine substitution (V1016G is associated with resistance to the type II pyrethroid. The result of this study will help us to strengthen basic and operational research on the­development­of­strategies­for­Dengue­vector­control­in­Indonesia.­This­study­utilized­an­allele-specificPolymerase Chain Reaction (AS­PCR assay that could be used to detect the V1016G

  20. To be or not to be the odd one out - Allele-specific transcription in pentaploid dogroses (Rosa L. sect. Caninae (DC. Ser

    Directory of Open Access Journals (Sweden)

    Theißen Günter

    2011-02-01

    Full Text Available Abstract Background Multiple hybridization events gave rise to pentaploid dogroses which can reproduce sexually despite their uneven ploidy level by the unique canina meiosis. Two homologous chromosome sets are involved in bivalent formation and are transmitted by the haploid pollen grains and the tetraploid egg cells. In addition the egg cells contain three sets of univalent chromosomes which are excluded from recombination. In this study we investigated whether differential behavior of chromosomes as bivalents or univalents is reflected by sequence divergence or transcription intensity between homeologous alleles of two single copy genes (LEAFY, cGAPDH and one ribosomal DNA locus (nrITS. Results We detected a maximum number of four different alleles of all investigated loci in pentaploid dogroses and identified the respective allele with two copies, which is presumably located on bivalent forming chromosomes. For the alleles of the ribosomal DNA locus and cGAPDH only slight, if any, differential transcription was determined, whereas the LEAFY alleles with one copy were found to be significantly stronger expressed than the LEAFY allele with two copies. Moreover, we found for the three marker genes that all alleles have been under similar regimes of purifying selection. Conclusions Analyses of both molecular sequence evolution and expression patterns did not support the hypothesis that unique alleles probably located on non-recombining chromosomes are less functional than duplicate alleles presumably located on recombining chromosomes.

  1. Detection of the BRAF V600E mutation in serous ovarian tumors: a comparative analysis of immunohistochemistry with a mutation-specific monoclonal antibody and allele-specific PCR.

    Science.gov (United States)

    Bösmüller, Hans; Fischer, Anna; Pham, Deborah L; Fehm, Tanja; Capper, David; von Deimling, Andreas; Bonzheim, Irina; Staebler, Annette; Fend, Falko

    2013-03-01

    Mutations of components of the mitogen-activated protein kinase pathway, mainly BRAF, are common in serous ovarian borderline tumors, whereas high-grade serous ovarian carcinomas rarely show this feature. With the advent of specific kinase inhibitors active against BRAF-mutated cancers, rapid and sensitive detection of the BRAF V600E, by far the most common mutation of this gene, is of great practical relevance. Currently, BRAF mutations are detected by DNA-based techniques. Recently, a monoclonal antibody (VE1) specific for the BRAF V600E protein suitable for archival tissues has been described. In this study, we compared detection of the V600E mutation in serous ovarian tumors by VE1 immunostaining and by allele-specific polymerase chain reaction. All 141 cases of high-grade serous ovarian cancer showed negative or rarely weak, diffuse background VE1 immunostaining, and BRAF wild type was confirmed by molecular analysis in all tested cases. In contrast, 1 (14%) of 7 low-grade serous carcinomas and 22 (71%) of 31 serous borderline tumors revealed moderate to strong VE1 positivity. Immunostaining was clearly evaluable in all cases with sufficient tumor cells, and only rare cases with narrow cytoplasm were difficult to interpret. The V600E mutation was confirmed by allele-specific polymerase chain reaction and sequencing in all VE1-positive cases. Two VE1-positive cases with low epithelial cell content required repeat microdissection to confirm the presence of the mutation. Immunohistochemistry with the VE1 antibody is a specific and sensitive tool for detection of the BRAF V600E mutation in serous ovarian tumors and may provide a practical screening test, especially in tumor samples with low epithelial content. PMID:23089489

  2. Changing patterns of basic household consumption in the Inner Mongolian grasslands: a case study of policy-oriented adoptive changes in the use of grasslands

    NARCIS (Netherlands)

    Du, B.; Zhen, L.; Groot, de R.S.; Goulden, C.E.; Long, X.; Cao, X.; Wu, R.; Sun, C.

    2014-01-01

    Grassland ecosystems, as the basic natural resources in the Inner Mongolia Autonomous Region, are becoming increasingly sensitive to human intervention, leading to deterioration in fragile ecosystems. The goal of this study was to describe the restoration policy-oriented adoptive changes to basic ho

  3. Monitoring Sub-Saharan African Physician Migration and Recruitment Post-Adoption of the WHO Code of Practice: Temporal and Geographic Patterns in the United States

    OpenAIRE

    Tankwanchi, Akhenaten Benjamin Siankam; Sten H Vermund; Douglas D Perkins

    2015-01-01

    Data monitoring is a key recommendation of the WHO Global Code of Practice on the International Recruitment of Health Personnel, a global framework adopted in May 2010 to address health workforce retention in resource-limited countries and the ethics of international migration. Using data on African-born and African-educated physicians in the 2013 American Medical Association Physician Masterfile (AMA Masterfile), we monitored Sub-Saharan African (SSA) physician recruitment into the physician...

  4. Allele Specific p53 Mutant Reactivation

    OpenAIRE

    Yu, Xin; Vazquez, Alexei; Levine, Arnold J.; Carpizo, Darren R.

    2012-01-01

    Rescuing the function of mutant p53 protein is an attractive cancer therapeutic strategy. Using the NCI anticancer drug screen data, we identified two compounds from the thiosemicarbazone family that manifest increased growth inhibitory activity in mutant p53 cells, particularly for the p53R175 mutant. Mechanistic studies reveal that NSC319726 restores WT structure and function to the p53R175 mutant. This compound kills p53R172H knock-in mice with extensive apoptosis and inhibits xenograft tu...

  5. Padrão de incorporação de fármacos antiretrovirais pelo sistema público de saúde no Brasil Patterns of antiretroviral therapy adoption by the Brazilian public health system

    Directory of Open Access Journals (Sweden)

    Paulo Schiavom Duarte

    2011-12-01

    Full Text Available OBJETIVO: avaliar o padrão de incorporação de fármacos antiretrovirais pelo Sistema Público de Saúde no Brasil (SUS. MÉTODOS: baseando-se em trabalhos prévios publicados na literatura médica foram propostos três indicadores a serem utilizados na análise da incorporação de fármacos: intervalo conhecimento-incorporação, massa crítica de conhecimento e intervalo validação-incorporação. Utilizando-se as bases de dados do Departamento de Informática do SUS (Datasus e do Sistema de Controle Logístico de Medicamentos (SICLOM foram selecionados dois grupos de tecnologias farmacológicas (medicações antiretrovirais e medicações de dispensação em caráter excepcional. RESULTADOS: os medicamentos antiretrovirais analisados foram incorporados de maneira mais precoce que os medicamentos de dispensação em caráter excepcional, tanto no que se refere ao intervalo "conhecimento-incorporação" quanto ao intervalo "validação-incorporação", e apresentam uma menor "massa crítica de conhecimento" para a incorporação. CONCLUSÃO: Os medicamentos antiretrovirais têm sido incorporados de maneira mais rápida, e após a publicação de um menor número de artigos científicos, quando em comparação aos medicamentos de dispensação em caráter excepcional.OBJECTIVE: The aim of the present scientific study is to evaluate the patterns of antiretroviral technology adoption by the Brazilian Public Health System (SUS. METHODS: Based on previous articles published in scientific medical literature, three indicators to assess antiretroviral technology adoption by SUS were proposed: knowledge-adoption interval; critical mass of knowledge; and validation-adoption interval. Using the databases from the SUS Department of Information Technology (DATASUS and from the Brazilian Logistic Center for Medication Control (SICLOM, two pharmaceutical groups were selected (antiretroviral medications and a group of high cost medications. RESULTS

  6. The − 5 A/G single-nucleotide polymorphism in the core promoter region of MT2A and its effect on allele-specific gene expression and Cd, Zn and Cu levels in laryngeal cancer

    Energy Technology Data Exchange (ETDEWEB)

    Starska, Katarzyna, E-mail: katarzyna.starska@umed.lodz.pl [I Department of Otolaryngology and Laryngological Oncology, Medical University of Łódź, Kopcinskiego 22, 90-153 Łódź (Poland); Krześlak, Anna; Forma, Ewa [Department of Cytobiochemistry, University of Łódź, Pomorska 142/143, 90-236 Łódź (Poland); Olszewski, Jurek [II Department of Otolaryngology and Laryngological Oncology, Medical University of Łódź, Żeromskiego 113, 90-549 Łódź (Poland); Morawiec-Sztandera, Alina [Department of Head and Neck Surgery, Medical University of Łódź, Paderewskiego 4, 93-509 Łódź (Poland); Aleksandrowicz, Paweł [Department of Otolaryngology and Laryngological Oncology, Medical University of Lublin, Jaczewskiego 8, 20-954 Lublin (Poland); Lewy-Trenda, Iwona [Department of Pathology, Medical University of Łódź, Pomorska 251, 92-213 Łódź (Poland); and others

    2014-10-15

    Metallothioneins (MTs) are low molecular weight, cysteine-rich heavy metal-binding proteins which participate in the mechanisms of Zn homeostasis, and protect against toxic metals. MTs contain metal-thiolate cluster groups and suppress metal toxicity by binding to them. The aim of this study was to determine the − 5 A/G (rs28366003) single-nucleotide polymorphism (SNP) in the core promoter region of the MT2A gene and to investigate its effect on allele-specific gene expression and Cd, Zn and Cu content in squamous cell laryngeal cancer (SCC) and non-cancerous laryngeal mucosa (NCM) as a control. The MT2A promoter region − 5 A/G SNP was determined by restriction fragment length polymorphism using 323 SCC and 116 NCM. MT2A gene analysis was performed by quantitative real-time PCR. The frequency of A allele carriage was 94.2% and 91.8% in SCC and NCM, respectively, while G allele carriage was detected in 5.8% and 8.2% of SCC and NCM samples, respectively. As a result, a significant association was identified between the − 5 A/G SNP in the MT2A gene with mRNA expression in both groups. Metal levels were analyzed by flame atomic absorption spectrometry. The significant differences were identified between A/A and both the A/G and G/G genotypes, with regard to the concentration of the contaminating metal. The Spearman rank correlation results showed that the MT2A expression and Cd, Zn, Cu levels were negatively correlated. Results obtained in this study suggest that − 5 A/G SNP in MT2A gene may have an effect on allele-specific gene expression and accumulation of metal levels in laryngeal cancer. - Highlights: • MT2A gene expression and metal content in laryngeal cancer tissues • Association between SNP (rs28366003) and expression of MT2A • Significant associations between the SNP and Cd, Zn and Cu levels • Negative correlation between MT2A gene expression and Cd, Zn and Cu levels.

  7. The − 5 A/G single-nucleotide polymorphism in the core promoter region of MT2A and its effect on allele-specific gene expression and Cd, Zn and Cu levels in laryngeal cancer

    International Nuclear Information System (INIS)

    Metallothioneins (MTs) are low molecular weight, cysteine-rich heavy metal-binding proteins which participate in the mechanisms of Zn homeostasis, and protect against toxic metals. MTs contain metal-thiolate cluster groups and suppress metal toxicity by binding to them. The aim of this study was to determine the − 5 A/G (rs28366003) single-nucleotide polymorphism (SNP) in the core promoter region of the MT2A gene and to investigate its effect on allele-specific gene expression and Cd, Zn and Cu content in squamous cell laryngeal cancer (SCC) and non-cancerous laryngeal mucosa (NCM) as a control. The MT2A promoter region − 5 A/G SNP was determined by restriction fragment length polymorphism using 323 SCC and 116 NCM. MT2A gene analysis was performed by quantitative real-time PCR. The frequency of A allele carriage was 94.2% and 91.8% in SCC and NCM, respectively, while G allele carriage was detected in 5.8% and 8.2% of SCC and NCM samples, respectively. As a result, a significant association was identified between the − 5 A/G SNP in the MT2A gene with mRNA expression in both groups. Metal levels were analyzed by flame atomic absorption spectrometry. The significant differences were identified between A/A and both the A/G and G/G genotypes, with regard to the concentration of the contaminating metal. The Spearman rank correlation results showed that the MT2A expression and Cd, Zn, Cu levels were negatively correlated. Results obtained in this study suggest that − 5 A/G SNP in MT2A gene may have an effect on allele-specific gene expression and accumulation of metal levels in laryngeal cancer. - Highlights: • MT2A gene expression and metal content in laryngeal cancer tissues • Association between SNP (rs28366003) and expression of MT2A • Significant associations between the SNP and Cd, Zn and Cu levels • Negative correlation between MT2A gene expression and Cd, Zn and Cu levels

  8. Monitoring Sub-Saharan African physician migration and recruitment post-adoption of the WHO code of practice: temporal and geographic patterns in the United States.

    Directory of Open Access Journals (Sweden)

    Akhenaten Benjamin Siankam Tankwanchi

    Full Text Available Data monitoring is a key recommendation of the WHO Global Code of Practice on the International Recruitment of Health Personnel, a global framework adopted in May 2010 to address health workforce retention in resource-limited countries and the ethics of international migration. Using data on African-born and African-educated physicians in the 2013 American Medical Association Physician Masterfile (AMA Masterfile, we monitored Sub-Saharan African (SSA physician recruitment into the physician workforce of the United States (US post-adoption of the WHO Code of Practice. From the observed data, we projected to 2015 with linear regression, and we mapped migrant physicians' locations using GPS Visualizer and ArcGIS. The 2013 AMA Masterfile identified 11,787 active SSA-origin physicians, representing barely 1.3% (11,787/940,456 of the 2013 US physician workforce, but exceeding the total number of physicians reported by WHO in 34 SSA countries (N = 11,519. We estimated that 15.7% (1,849/11,787 entered the US physician workforce after the Code of Practice was adopted. Compared to pre-Code estimates from 2002 (N = 7,830 and 2010 (N = 9,938, the annual admission rate of SSA émigrés into the US physician workforce is increasing. This increase is due in large part to the growing number of SSA-born physicians attending medical schools outside SSA, representing a trend towards younger migrants. Projection estimates suggest that there will be 12,846 SSA migrant physicians in the US physician workforce in 2015, and over 2,900 of them will be post-Code recruits. Most SSA migrant physicians are locating to large urban US areas where physician densities are already the highest. The Code of Practice has not slowed the SSA-to-US physician migration. To stem the physician "brain drain", it is essential to incentivize professional practice in SSA and diminish the appeal of US migration with bolder interventions targeting primarily early-career (age ≤ 35 SSA

  9. Monitoring Sub-Saharan African physician migration and recruitment post-adoption of the WHO code of practice: temporal and geographic patterns in the United States.

    Science.gov (United States)

    Tankwanchi, Akhenaten Benjamin Siankam; Vermund, Sten H; Perkins, Douglas D

    2015-01-01

    Data monitoring is a key recommendation of the WHO Global Code of Practice on the International Recruitment of Health Personnel, a global framework adopted in May 2010 to address health workforce retention in resource-limited countries and the ethics of international migration. Using data on African-born and African-educated physicians in the 2013 American Medical Association Physician Masterfile (AMA Masterfile), we monitored Sub-Saharan African (SSA) physician recruitment into the physician workforce of the United States (US) post-adoption of the WHO Code of Practice. From the observed data, we projected to 2015 with linear regression, and we mapped migrant physicians' locations using GPS Visualizer and ArcGIS. The 2013 AMA Masterfile identified 11,787 active SSA-origin physicians, representing barely 1.3% (11,787/940,456) of the 2013 US physician workforce, but exceeding the total number of physicians reported by WHO in 34 SSA countries (N = 11,519). We estimated that 15.7% (1,849/11,787) entered the US physician workforce after the Code of Practice was adopted. Compared to pre-Code estimates from 2002 (N = 7,830) and 2010 (N = 9,938), the annual admission rate of SSA émigrés into the US physician workforce is increasing. This increase is due in large part to the growing number of SSA-born physicians attending medical schools outside SSA, representing a trend towards younger migrants. Projection estimates suggest that there will be 12,846 SSA migrant physicians in the US physician workforce in 2015, and over 2,900 of them will be post-Code recruits. Most SSA migrant physicians are locating to large urban US areas where physician densities are already the highest. The Code of Practice has not slowed the SSA-to-US physician migration. To stem the physician "brain drain", it is essential to incentivize professional practice in SSA and diminish the appeal of US migration with bolder interventions targeting primarily early-career (age ≤ 35) SSA physicians. PMID

  10. Analysis of the relationship between genomic GC Content and patterns of base usage, codon usage and amino acid usage in prokaryotes: similar GC content adopts similar compositional frequencies regardless of the phylogenetic lineages.

    Directory of Open Access Journals (Sweden)

    Hui-Qi Zhou

    Full Text Available The GC contents of 2670 prokaryotic genomes that belong to diverse phylogenetic lineages were analyzed in this paper. These genomes had GC contents that ranged from 13.5% to 74.9%. We analyzed the distance of base frequencies at the three codon positions, codon frequencies, and amino acid compositions across genomes with respect to the differences in the GC content of these prokaryotic species. We found that although the phylogenetic lineages were remote among some species, a similar genomic GC content forced them to adopt similar base usage patterns at the three codon positions, codon usage patterns, and amino acid usage patterns. Our work demonstrates that in prokaryotic genomes: a base usage, codon usage, and amino acid usage change with GC content with a linear correlation; b the distance of each usage has a linear correlation with the GC content difference; and c GC content is more essential than phylogenetic lineage in determining base usage, codon usage, and amino acid usage. This work is exceptional in that we adopted intuitively graphic methods for all analyses, and we used these analyses to examine as many as 2670 prokaryotes. We hope that this work is helpful for understanding common features in the organization of microbial genomes.

  11. Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP to detect the L1014F kdr-w mutation in Anopheles gambiae s. l.

    Directory of Open Access Journals (Sweden)

    Badolo Athanase

    2012-07-01

    Full Text Available Abstract Background Malaria control relies heavily on treated bed nets and indoor residual spraying with pyrethroid insecticides. Unfortunately, the resistance to pyrethroid insecticides, mainly due to the kdr mutation, is spreading in the main malaria vector Anopheles gambiae s.l., decreasing the insecticides’ efficacy. To manage the insecticide resistance rapidly and flexibly, simple and effective tools for the early detection of resistant mosquitoes are needed. This study aimed to develop an allele-specific, loop-mediated, isothermal amplification (AS-LAMP method to detect the West African-type kdr mutation (kdr-w; L1014F in field-collected mosquitoes. Methods DNA fragments of the wild-type and the mutated kdr gene were used to select the primers and develop the method. The primers were designed with the mutation at the 5’ end of the backward inner primer (BIP. The AS-LAMP method was compared to the AS-PCR method using the genomic DNA of 120 field-collected mosquitoes. Results The AS-LAMP method could discriminate between the wild-type homozygote, the heterozygote, and the kdr-w homozygote within 75 min. The AS-LAMP method has the advantage of being faster and at least as sensitive and specific as the AS-PCR method. Conclusions The AS-LAMP method can be used to detect the kdr mutation for quick decision-making, even in less well-equipped laboratories.

  12. The Pattern and Process of Adoption and Scaling up: Variation in Project Outcome Reveals the Importance of Multilevel Collaboration in Agroforestry Development

    Directory of Open Access Journals (Sweden)

    Karl-Erik Johansson

    2013-12-01

    Full Text Available Agroforestry is considered a subsistence system that balances the urgent need for food and income of small scale farmers with restoration and conservation of ecosystem services, and climate change adaptation and mitigation. The Vi Agroforestry Program aims to implement agroforestry as a means to alleviate poverty and increase resilience among the poorest smallholders. After seven years, the Vi Agroforestry Project in the Mara Region of Tanzania had an inter-village variation in the proportion of households with tangible surviving agroforestry trees ranging from 10%–90%. Using a multiple methods approach, this variation was analysed in relation to changes and differences among administrative districts and project zones regarding perceived barriers to agroforestry adoption, project interventions, governance and the chronology of the process. In districts and zones where collaboration among the project staff, government counterparts and other stakeholders had been established at multiple levels, more agroforestry trees survived and a larger proportion of households practiced agroforestry. The established collaboration made it possible to discover and consider opportunities and barriers to agroforestry development such as diverse stakeholder interests and perceptions. As a result, potential conflicts could be avoided and socially robust solutions developed, adapted and integrated into the local subsistence systems.

  13. Temporal pattern of ICAM-I mediated regulatory T cell recruitment to sites of inflammation in adoptive transfer model of multiple sclerosis.

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    Sebastian Doerck

    Full Text Available Migration of immune cells to the target organ plays a key role in autoimmune disorders like multiple sclerosis (MS. However, the exact underlying mechanisms of this active process during autoimmune lesion pathogenesis remain elusive. To test if pro-inflammatory and regulatory T cells migrate via a similar molecular mechanism, we analyzed the expression of different adhesion molecules, as well as the composition of infiltrating T cells in an in vivo model of MS, adoptive transfer experimental autoimmune encephalomyelitis in rats. We found that the upregulation of ICAM-I and VCAM-I parallels the development of clinical disease onset, but persists on elevated levels also in the phase of clinical remission. However, the composition of infiltrating T cells found in the developing versus resolving lesion phase changed over time, containing increased numbers of regulatory T cells (FoxP3 only in the phase of clinical remission. In order to test the relevance of the expression of cell adhesion molecules, animals were treated with purified antibodies to ICAM-I and VCAM-I either in the phase of active disease or in early remission. Treatment with a blocking ICAM-I antibody in the phase of disease progression led to a milder disease course. However, administration during early clinical remission aggravates clinical symptoms. Treatment with anti-VCAM-I at different timepoints had no significant effect on the disease course. In summary, our results indicate that adhesion molecules are not only important for capture and migration of pro-inflammatory T cells into the central nervous system, but also permit access of anti-inflammatory cells, such as regulatory T cells. Therefore it is likely to assume that intervention at the blood brain barrier is time dependent and could result in different therapeutic outcomes depending on the phase of CNS lesion development.

  14. Detection of BRAF Mutations Using a Fully Automated Platform and Comparison with High Resolution Melting, Real-Time Allele Specific Amplification, Immunohistochemistry and Next Generation Sequencing Assays, for Patients with Metastatic Melanoma.

    Directory of Open Access Journals (Sweden)

    Alexandre Harlé

    Full Text Available Metastatic melanoma is a severe disease with one of the highest mortality rate in skin diseases. Overall survival has significantly improved with immunotherapy and targeted therapies. Kinase inhibitors targeting BRAF V600 showed promising results. BRAF genotyping is mandatory for the prescription of anti-BRAF therapies.Fifty-nine formalin-fixed paraffin-embedded melanoma samples were assessed using High-Resolution-Melting (HRM PCR, Real-time allele-specific amplification (RT-ASA PCR, Next generation sequencing (NGS, immunohistochemistry (IHC and the fully-automated molecular diagnostics platform IdyllaTM. Sensitivity, specificity, positive predictive value and negative predictive value were calculated using NGS as the reference standard to compare the different assays.BRAF mutations were found in 28(47.5%, 29(49.2%, 31(52.5%, 29(49.2% and 27(45.8% samples with HRM, RT-ASA, NGS, IdyllaTM and IHC respectively. Twenty-six (81.2% samples were found bearing a c.1799T>A (p.Val600Glu mutation, three (9.4% with a c.1798_1799delinsAA (p.Val600Lys mutation and one with c.1789_1790delinsTC (p.Leu597Ser mutation. Two samples were found bearing complex mutations.HRM appears the less sensitive assay for the detection of BRAF V600 mutations. The RT-ASA, IdyllaTM and IHC assays are suitable for routine molecular diagnostics aiming at the prescription of anti-BRAF therapies. IdyllaTM assay is fully-automated and requires less than 2 minutes for samples preparation and is the fastest of the tested assays.

  15. Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients.

    Directory of Open Access Journals (Sweden)

    Sirirat Seekhuntod

    Full Text Available Patients with KRAS mutations do not respond to epidermal growth factor receptor (EGFR inhibitors and fail to benefit from adjuvant chemotherapy. Mutation analysis of KRAS is needed before starting treatment with monoclonal anti-EGFR antibodies in patients with metastatic colorectal cancer (mCRC. The objective of this study is to develop a multiplex allele-specific PCR (MAS-PCR assay to detect KRAS mutations.We developed a single-tube MAS-PCR assay for the detection of seven KRAS mutations (G12D, G12A, G12R, G12C, G12S, G12V, and G13D. We performed MAS-PCR assay analysis for KRAS on DNA isolated from 270 formalin-fixed paraffin-embedded (FFPE colorectal cancer tissues. Sequences of all 270 samples were determined by pyrosequencing. Seven known point-mutation DNA samples diluted with wild-type DNA were assayed to determine the limitation of detection and reproducibility of the MAS-PCR assay.Overall, the results of MAS-PCR assay were in good concordance with pyrosequencing, and only seven discordant samples were found. The MAS-PCR assay reproducibly detected 1 to 2% mutant alleles. The most common mutations were G13D in codon 13 (49.17%, G12D (25.83% and G12V (12.50% in codon 12.The MAS-PCR assay provides a rapid, cost-effective, and reliable diagnostic tool for accurate detection of KRAS mutations in routine FFPE colorectal cancer tissues.

  16. Adoption of Preoperative Radiation Therapy for Rectal Cancer From 2000 to 2006: A Surveillance, Epidemiology, and End Results Patterns-of-Care Study

    International Nuclear Information System (INIS)

    study, there was widespread, rapid adoption of preoperative RT for locally advanced rectal cancer. However, preoperative RT may be underused in certain sociodemographic groups.

  17. Adoption & Foster Care

    Science.gov (United States)

    ... Children > Family Life > Family Dynamics > Adoption & Foster Care Adoption & Foster Care Article Body ​Each year, many children join families through adoption and foster care. These families may face unique ...

  18. Analyzing Adoption and Replacement of Rice Varieties in NSW

    OpenAIRE

    Singh, Inder Pal; Brennan, John P.

    1997-01-01

    The pattern of adoption and disadoption of a new research outcome has considerable impact on the total benefits to the industry from such research. The aims in this paper are to examine the pattern of adoption of individual rice varieties developed in NSW, and to develop an estimate of the expected adoption curve for different types of rice varieties. The analysis indicates that the pattern of adoption and disadoption of rice varieties can be modeled successfully. A framework is developed for...

  19. Becoming an Adoptive Parent

    DEFF Research Database (Denmark)

    McIlvenny, Paul; Raudaskoski, Pirkko Liisa

    in transnational adoption practice and representation. We present the results of our analysis of how the experiences of adoptive parents are (re)mediated in a Danish television documentary series following five prospective adoptive couples, not all of whom succeed in their 'quest' to adopt from abroad. Furthermore...... those practices which may precipitate a 'call for help' to distant actors, such as social welfare provision or counselling services....

  20. Adoption in ancient times

    OpenAIRE

    Bisha Eugena

    2015-01-01

    Since in ancient times, in all human cultures, children transfered from biological parents to parents that want them to create family, for political alliances, for inheritance, for a future marriage, or to care for elderly parents. The practice of adoption was fairly common in different places and periods. Adoption is mention on Bible and Quran. Greeks, Romans, Egyptians and Babylonians had adoption systems.

  1. Companion animal adoption study.

    Science.gov (United States)

    Neidhart, Laura; Boyd, Renee

    2002-01-01

    To better understand the outcomes of companion animal adoptions, Bardsley & Neidhart Inc. conducted a series of 3 surveys over a 1-year period with dog and cat owners who had adopted their pet through either a (a) Luv-A-Pet location, (b) Adopt-a-thon, or (c) traditional shelter. This article suggests opportunities to improve owners' perceptions of their pets and the adoption process through (a) providing more information before adoption about pet health and behaviors, (b) providing counseling to potential adopters to place pets appropriately, and (c) educating adopters to promote companion animal health and retention. Results demonstrate that the pet's relationship to the family unit, such as where the pet sleeps and how much time is spent with the pet, is related to the amount of veterinary care the companion animal receives, and to long-term retention. Satisfaction and retention are attributed to the pet's personality, compatibility, and behavior, rather than demographic differences among adopters or between adoption settings. The age of the companion animal at adoption, the intended recipient, and presence of children in the home also play a role. Health problems were an issue initially for half of all adopted pets, but most were resolved within 12 months. Roughly one fourth of adopters who no longer have their companion animal said their pet died. Characteristics of pets that died support the contention that spaying and neutering profoundly affects a companion animal's life span. Although retention is similar for dogs and cats, mortality is higher among cats in the first year after adoption. PMID:12578739

  2. The Value of Adoption

    OpenAIRE

    Mary Eschelbach Hansen

    2006-01-01

    The human services cost of adoption is about half the cost of long-term foster care for children whose birth parents’ rights have been terminated. Because adoption is an effective intervention for improving a variety of outcomes for those exposed to adverse childhood experiences, the total savings to government in areas such as special education and criminal justice is of the same magnitude as the child welfare savings. The private benefit to adopted children in terms of additional income ear...

  3. The claim from adoption.

    Science.gov (United States)

    Petersen, Thomas Sobirk

    2002-08-01

    In this article several justifications of what I call 'the claim from adoption' are examined. The claim from adoption is that, instead of expending resources on bringing new children into the world using reproductive technology and then caring for these children, we ought to devote these resources to the adoption and care of existing destitute children. Arguments trading on the idea that resources should be directed to adoption instead of assisted reproduction because already existing people can benefit from such a use of resources whereas we cannot benefit individuals by bringing them into existence are rejected. It is then argued that a utilitarian argument proposed by Christian Munthe that supports the claim from adoption in some situations should be rejected because the support it offers does not extend to certain situations in which it seems morally obvious that resources should be expended on adoption rather than assisted reproduction. A version of the Priority View improves upon Munthe's utilitarianism by supporting the claim from adoption in the cases in which Munthe's argument failed. Some allegedly counterintuitive implications of the Priority View are then discussed, and it is concluded that the Priority View is more plausible than utilitarianism. In a concluding section on policy issues it is argued that, even though the claim from adoption can be justified in a variety of situations, it does not follow that, in these situations, governments should direct resources away from assisted reproduction and towards adoption.

  4. The Danish Adoption Register

    DEFF Research Database (Denmark)

    Petersen, Liselotte; Sørensen, Thorkild I A

    2011-01-01

    The Danish Adoption Register was established in 1963-1964 to explore the genetic and environmental contribution to familial aggregation of schizophrenia.......The Danish Adoption Register was established in 1963-1964 to explore the genetic and environmental contribution to familial aggregation of schizophrenia....

  5. Adopt Your Watershed

    Data.gov (United States)

    U.S. Environmental Protection Agency — Adopt Your Watershed is a Website that encourages stewardship of the nation's water resources and serves as a national inventory of local watershed groups and...

  6. The Colorado Adoption Project.

    Science.gov (United States)

    Plomin, R; DeFries, J C

    1983-04-01

    This report provides an overview of the Colorado Adoption Project (CAP), a longitudinal, prospective, multivariate adoption study of behavioral development. Examples of the types of analyses that can be conducted using this design are presented. The examples are based on general cognitive-ability data for adoptive, biological, and control parents; assessments of their home environment; and Bayley Mental Development Index scores for 152 adopted children and 120 matched control children tested at both 1 and 2 years of age. The illustrative analyses include matched control children tested at both 1 and 2 years of age. The illustrative analyses include examination of genetic and environmental sources of variance, identification of environmental influence devoid of genetic bias, assessment of genotype-environment interaction and correlation, and analyses of the etiology of change and continuity in development.

  7. Travelers' Health: International Adoption

    Science.gov (United States)

    ... any child with fever and bloody diarrhea. Unlike refugees, internationally adopted children are not treated for parasites ... disease is endemic throughout much of Mexico, Central America, and South America (Chapter 3, Trypanosomiasis, American (Chagas ...

  8. The OER Adoption Pyramid

    OpenAIRE

    Trotter, Henry; Cox, Glenda

    2016-01-01

    This Pyramid was developed in the course of a research paper focusing on why South African academics adopt OER or not. We understood that numerous factors shaped their choices, but it became apparent that some factors were "essential" to OER activity while others were merely "influential". To clarify which factors were required for any type of OER activity, we developed the OER Adoption Pyramid, which consolidates the factors into six hierarchically related categories: acc...

  9. Researching Multipath TCP Adoption

    OpenAIRE

    Warma, Henna; Hämmäinen, Heikki

    2010-01-01

    International audience The adoption process of a new Internet protocol or only a change to an existing one is anything but trivial. The classical diffusion theory does not apply as such for studying protocol adoption because the deployment of a protocol usually requires the involvement of multiple stakeholders with varying interests. Multipath TCP (MPTCP) is a new interesting change to the TCP/IP protocol suite which is an extension to regular TCP. MPTCP exploits the idea of resource pooli...

  10. Beyond the adoption/ non-adoption dichotomy: the impact of innovation characteristics on potential adopters' transition through adoption process stages

    OpenAIRE

    Frambach, R T; Agarwal, M.K.; Nijssen, E.J.

    2002-01-01

    Research on innovation adoption has suffered from a bias towards understanding the factors that affect the dichotomous adoption/non-adoption decision. Much less attention is devoted to the question why potential adopters fail to progress to the adoption stage from earlier stages in the decision making process. Such knowledge is essential to understand what factors actually underlie the non-adoption of an innovation. As perceived innovation characteristics have been found to influence adoption...

  11. Questions about Adoption

    Science.gov (United States)

    ... Dynamics Adoption & Foster Care Communication & Discipline Types of Families Media Work & Play Getting Involved in Your Community Healthy Children > ... on. Find other words that everyone in your family is comfortable with. The terms birth mother and birth father are very common. Biological parents ...

  12. Beyond the adoption/ non-adoption dichotomy: the impact of innovation characteristics on potential adopters' transition through adoption process stages

    NARCIS (Netherlands)

    Agarwal, M.K.; Frambach, R.T.

    2002-01-01

    Research on innovation adoption has suffered from a bias towards understanding the factors that affect the dichotomous adoption/non-adoption decision.Much less attention is devoted to the question why potential adopters fail to progress to the adoption stage from earlier stages in the decision makin

  13. Adoption of Children With Disabilities: A Study With Adoptive Parents

    OpenAIRE

    Gisele De Mozzi; Adriano Henrique Nuernberg

    2016-01-01

    Abstract Taking into consideration the supremacy of a child's profile often chosen by adoptive parents, this study aimed to understand the adoption of children with disabilities from the perspective of adoptive parents who have experienced this practice. The participants were eleven adoptive families of disabled children, all of whom had knowledge about the health conditions of the adoptive children at the time of adoption. The instruments used were a semi-structured interview and a sociodemo...

  14. Adoption of Broadband Services

    DEFF Research Database (Denmark)

    Falch, Morten

    2008-01-01

    Broadband is seen as a key infrastructure for developing the information society. For this reason many Governments are actively engaged in stimulating investments in broadband infrastructures and use of broadband services. This chapter compares a wide range of broadband strategies in the most suc....... Many countries have provided active support for stimulating diffusion of broadband and national variants of this type of policies in different countries are important for an explanation of national differences in adoption of broadband....

  15. Adoption, nation, migration

    DEFF Research Database (Denmark)

    Müller, Anders Riel

    2013-01-01

    Som transnationalt adopteret vokser man ofte op med en fortælling om, at man er født i et fattigt land. Og at ens første forældre var fattige eller oplevede så store problemer, at de ikke så andre muligheder end at afgive en til adoption. Det er en historie, man bliver fortalt igen og igen. Og so...

  16. Microeconomics of Technology Adoption

    OpenAIRE

    Foster, Andrew D.; Rosenzweig, Mark R.

    2010-01-01

    There is an emerging consensus among macro-economists that differences in technology across countries accounts for the major differences in per-capita GDP and the wages of workers with similar skills across countries. Accounting for differences in technology levels across countries thus can go a long way towards understanding global inequality. One mechanism by which poorer countries can catch up with richer countries is through technological diffusion, the adoption by low-income countries of...

  17. Hospitious Adoption: How Hospitality Empowers Children and Transforms Adoption

    Science.gov (United States)

    Gritter, James L.

    2009-01-01

    Building on previous books by the author, "Hospitious Adoption: How Hospitality Empowers Children and Transforms Adoption" examines the next step after open adoption. Gritter takes the approach that practicing goodwill, respect, and courage within the realm of adoption makes the process move smoother and enriches children's lives. Following a…

  18. Allele-specific suppression of the temperature sensitivity of fitA/fitB mutants of Escherichia coli by a new mutation (fitC4): isolation, characterization and its implications in transcription control

    Indian Academy of Sciences (India)

    S Vidya; B Praveen Kamalakar; M Hussain Munavar; L Sathish Kumar; R Jayaraman

    2006-03-01

    The temperature sensitive transcription defective mutant of Escherichia coli originally called fitA76 has been shown to harbour two missense mutations namely pheS5 and fit95. In order to obtain a suppressor of fitA76, possibly mapping in rpoD locus, a Ts+ derivative (JV4) was isolated from a fitA76 mutant. It was found that JV4 neither harbours the lesions present in the original fitA76 nor a suppressor that maps in or near rpoD. We show that JV4 harbours a modified form of fitA76 (designated fitA76*) together with its suppressor. The results presented here indicate that the fit95 lesion is intact in the fitA76* mutant and the modification should be at the position of pheS5. Based on the cotransduction of the suppressor mutation and/or its wild type allele with pps, aroD and zdj-3124::Tn10 kan we have mapped its location to 39⋅01 min on the E. coli chromosome. We tentatively designate the locus defined by this new extragenic suppressor as fitC and the suppressor allele as fitC4. While fitC4 could suppress the Ts phenotype of fitA76* present in JV4, it fails to suppress the Ts phenotype of the original fitA76 mutant (harbouring pheS5 and fit95). Also fitC4 could suppress the Ts phenotype of a strain harbouring only pheS5. Interestingly, the fitC4 Ts phenotype could also be suppressed by fit95. The pattern of decay of pulse labelled RNA in the strains harbouring fitC4 and the fitA76* resembles that of the original fitA76 mutant implying a transcription defect similar to that of fitA76 in both these mutants. The implications of these findings with special reference to transcription control by Fit factors in vivo are discussed.

  19. Conduct Problems in Adopted and Non-adopted Adolescents and Adoption Satisfaction as a Protective Factor

    OpenAIRE

    Nilsson, Renea; Rhee, Soo Hyun; Corley, Robin P.; Rhea, Sally-Ann; Wadsworth, Sally J.; DeFries, John C.

    2011-01-01

    The present study compared the level of conduct problems at age 17 in a large, non-clinical sample of adopted participants placed in infancy and children in non-adoptive families matched to the adoptive families on demographic characteristics. Higher levels of adolescent and parent adoption satisfaction were associated with lower levels of conduct problems. Gender by adoption status interactions were not significant. However, female adopted participants had higher levels of conduct problems t...

  20. The Child Adoption Marketplace: Parental Preferences and Adoption Outcomes

    OpenAIRE

    Skidmore, Mark; Anderson, Gary; Eiswerth, Mark

    2011-01-01

    In the United States child adoption costs vary considerably, ranging from no out-of-pocket expense to $50,000 or more. What are the underlying causes for the variability in child adoption expenses? While cost variability is widely acknowledged, the sources of the differentials have not been systematically examined. This research considers the possibility that adoption cost differentials are determined by adoptive parent preferences for adoptive child characteristics. We administered a detaile...

  1. Adoption Research: Trends, Topics, Outcomes

    Science.gov (United States)

    Palacios, Jesus; Brodzinsky, David

    2010-01-01

    The current article provides a review of adoption research since its inception as a field of study. Three historical trends in adoption research are identified: the first focusing on risk in adoption and identifying adoptee-nonadoptee differences in adjustment; the second examining the capacity of adopted children to recover from early adversity;…

  2. Åben Adoption

    DEFF Research Database (Denmark)

    Jeldtoft, Nadia

    2007-01-01

    barn adopteres, jo mere stabil og uproblematisk bliver relationen mellem barn og adoptivforældre. Samtidig peger undersøgelserne på, at adoptioner med høj grad af åbenhed og kontakt mellem barn, adoptivforældrene og de biologiske forældre fungerer bedst. Hermed rokkes ved en udbredt forestilling om......Denne rapport er en systematisk forskningsoversigt over udenlandske erfaringer med adoption i forhold til anbringelse uden for hjemmet, fx familiepleje, institutionsanbringelse og hjemgivelse til de biologiske forældre. Konklusionerne i rapporten er overraskende entydige: Adopterede børn klarer sig...

  3. Broadband adoption by SMES

    OpenAIRE

    Oni, Oluwasola

    2007-01-01

    This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University. Because the benefits of broadband for businesses have been widely publicized, the UK government has tried to ensure that there is a wide and fast take-up of the technology. Initial figures showed that broadband adoption by SMEs was particularly slow and there has been little research on the use of broadband by businesses, particularly SMEs. An in-depth study into the roles and activities of t...

  4. Process of adoption communication openness in adoptive families: adopters’ perspective

    Directory of Open Access Journals (Sweden)

    Maria Acciaiuoli Barbosa-Ducharne

    2016-01-01

    Full Text Available Abstract Communication about adoption is a family interaction process which is more than the simple exchange of information. Adoption communication can be characterized in terms of the level of openness of family conversations regarding the child’s past and the degree of the family’s adoption social disclosure. The objective of this study is to explore the process of adoption communication openness in Portuguese adoptive families by identifying the impact of variables related to the adoption process, the adoptive parenting and the adoptee. One hundred twenty five parents of children aged 3 to 15, who were adopted on average 4 years ago, participated in this study. Data was collected during home visits using the Parents Adoption Process Interview. A cluster analysis identified three different groups of families according to the level of adoption communication openness within the family and outside. The findings also showed that the process of the adoption communication openness started when parents decided to adopt, developed in parent-child interaction and was susceptible to change under professional intervention. The relevance of training given to prospective adopters and of professional practice based on scientific evidence is highlighted.

  5. Special topics in international adoption.

    Science.gov (United States)

    Jenista, Jerri Ann

    2005-10-01

    As international adoption has become more "mainstream," the issues recently addressed in domestic adoption have become more important in adoptions involving children originating in other countries. Certain groups of prospective adoptive parents, such as gay or lesbian couples, single parents, and parents with disabilities, have begun to apply to adopt in ever increasing numbers. Children who may have been considered unadoptable in the past are now routinely being offered to prospective adoptive parents. The numbers and ages of the children placed and the spacing between adoptions have come under scrutiny. The rates of adoption dissolutions and disruptions are being examined carefully by the receiving and sending countries. There is a pressing need for research into numerous social aspects of adoption. PMID:16154473

  6. Multiplex allele-specific target amplification based on PCR suppression

    OpenAIRE

    Broude, Natalia E.; Zhang, Lingang; Woodward, Karen; Englert, David; Cantor, Charles R.

    2001-01-01

    We have developed a strategy for multiplex PCR based on PCR suppression. PCR suppression allows DNA target amplification with only one sequence-specific primer per target and a second primer that is common for all targets. Therefore, an n-plex PCR would require only n + 1 primers. We have demonstrated uniform, efficient amplification of targeted sequences in 14-plex PCR. The high specificity of suppression PCR also provides multiplexed amplification with allele specifi...

  7. International Adoption: A Way Forward

    OpenAIRE

    Bartholet, Elizabeth

    2011-01-01

    This is the introduction to a special issue of articles on international adoption, summing up their relevance to the debate in the field. International adoption is in turmoil, with a dramatic reduction in recent years in the number of children placed in adoptive homes. These articles provide important new support for international adoption as an appropriate way to advance children’s rights and interests. And they provide information about ways to address any problems of corruption and abus...

  8. Adoption Legislation Consultation - Discussion Paper

    OpenAIRE

    Department of Health

    2003-01-01

    Adoption Legislation Consultation РDiscussion Paper This discussion paper is part of a fundamental review of Irish adoption policy and practice referred to in the Minister for Children̢?Ts foreword. The purpose of the review is to give organisations and individuals with an interest in adoption law and practice an opportunity to contribute to the development of a modern adoption system characterised by clarity, consistency and fairness. Click here to download PDF 104kb

  9. The Texas Adoption Project: adopted children and their intellectual resemblance to biological and adoptive parents.

    Science.gov (United States)

    Horn, J M

    1983-04-01

    Intelligence test scores were obtained from parents and children in 300 adoptive families and compared with similar measures available for the biological mothers of the same adopted children. Results supported the hypothesis that genetic variability is an important influence in the development of individual differences for intelligence. The most salient finding was that adopted children resemble their biological mothers more than they resemble the adoptive parents who reared them from birth. A small subset of the oldest adopted children did not resemble their biological mothers. The suggestion that the influence of genes declines with age is treated with caution since other adoption studies report a trend in the opposite direction.

  10. A Phenomenological Exploration of Adoption

    Science.gov (United States)

    Baltimore, Diana L.; Crase, Sedahlia Jasper

    2009-01-01

    This qualitative analysis explored children's and adults' experiences with adoption. We used phenomenological methodology and individually interviewed 25 participants and included adoptive mothers and fathers, and their children, each adopted before 18 months of age. Two research questions guided the data analysis: (a) What are children's and…

  11. Adoption Resource Directory: Region X.

    Science.gov (United States)

    1983

    State, regional, and national adoption resources are described in this directory for residents of Region X states (Alaska, Idaho, Oregon, and Washington). Emphasizing the adoption of children with special needs, the directory gives organizational contacts for parents in various stages of the adoption process and mentions resources for social…

  12. Adoption Resources for Black Children

    Science.gov (United States)

    Gallagher, Ursula M.

    1971-01-01

    The growing number of adoptions in this country, including racially mixed adoptions, attest to the general acceptance of adoption as a way of bringing love to children in need of families of their own and the satisfactions of parenthood to childless couples, single men and women, and families who have room for one more. (Author/AJ)

  13. Dogmatism and Attitudes Toward Adoption

    Science.gov (United States)

    Dembroski, Betty G.; Johnson Dale L.

    1969-01-01

    Using Rokeach's Dogmatism Scale and an Adoption Attitude Scale administered to 113 college students study supports hypothesis that among males dogmatism and intolerance toward areas relating to adoption would be positively correlated. Negative correlation for females suggests that emphasis on maternal role makes adoption attitudes exception to…

  14. Prescriber adoption of newly approved selective COX-2 inhibitors

    NARCIS (Netherlands)

    Layton, Deborah; Souverein, Patrick C.; Heerdink, Eibert R.; Shakir, Saad A. W.; Egberts, A. G. C.

    2008-01-01

    Introduction There is no consistent definition of prescribers who adopt new drug treatments early. This study examines if COX-2 inhibitors (coxibs) were prescribed by subsets of practitioners and describes GP adoption patterns of coxibs and existing NSAIDs over time. Methods A population-based drug

  15. Technology adoption and the impact on average productivity

    NARCIS (Netherlands)

    Hellegers, P.J.G.J.; Zeng, D.; Zilberman, D.

    2011-01-01

    In this paper, a framework is developed to analyze how the specifications of new technologies and the heterogeneity of micro-units of production affect the input use, the adoption pattern, and the productivity of inputs. It shows that asset-productivity-enhancing (APE) technologies tend to be adopte

  16. Adoption of mobile banking in Finland

    OpenAIRE

    Suoranta, Mari

    2003-01-01

    Banking is an area in which technology has had a fundamental impact on the everyday lives of consumers. This article-based dissertation examines an innovation in the financial services industry, namely mobile banking services. It seeks to determine and explain the factors affecting the adoption of mobile banking services, thus the perspective taken by the dissertation is one that focuses on consumer behaviour patterns. In order to arrive at a theoretical model, it draws on traditional theorie...

  17. Intellectual resemblance among adoptive adoptive and biological relatives: the Texas adoption project.

    Science.gov (United States)

    Horn, J M; Loehlin, J C; Willerman, L

    1979-05-01

    Intellectual and personality measures were available from unwed mothers who gave their children up for adoption at birth. The same or similar measures have been obtained from 300 sets of adoptive parents and all of their adopted and natural children in the Texas Adoption Project. The sample characteristics are discussed in detail, and the basic findings for IQ are presented. Initial analyses of the data on IQ suggest moderate heritabilities. Emphasis is placed on the preliminary nature of these findings.

  18. Technology Adoption: an Interaction Perspective

    Science.gov (United States)

    Sitorus, Hotna M.; Govindaraju, Rajesri; Wiratmadja, I. I.; Sudirman, Iman

    2016-02-01

    The success of a new technology depends on how well it is accepted by its intended users. Many technologies face the problem of low adoption rate, despite the benefits. An understanding of what makes people accept or reject a new technology can help speed up the adoption rate. This paper presents a framework for technology adoption based on an interactive perspective, resulting from a literature study on technology adoption. In studying technology adoption, it is necessary to consider the interactions among elements involved in the system, for these interactions may generate new characteristics or new relationships. The interactions among elements in a system adoption have not received sufficient consideration in previous studies of technology adoption. Based on the proposed interaction perspective, technology adoption is elaborated by examining interactions among the individual (i.e. the user or prospective user), the technology, the task and the environment. The framework is formulated by adopting several theories, including Perceived Characteristics of Innovating, Diffusion of Innovation Theory, Technology Acceptance Model, Task-Technology Fit and usability theory. The proposed framework is illustrated in the context of mobile banking adoption. It is aimed to offer a better understanding of determinants of technology adoption in various contexts, including technology in manufacturing systems.

  19. Predicting Virtual Learning Environment Adoption

    DEFF Research Database (Denmark)

    Penjor, Sonam; Zander, Pär-Ola Mikael

    2016-01-01

    This study investigates the significance of Rogers’ Diffusion of Innovations (DOI) theory with regard to the use of a Virtual Learning Environment (VLE) at the Royal University of Bhutan (RUB). The focus is on different adoption types and characteristics of users. Rogers’ DOI theory is applied...... to investigate the influence of five predictors (relative advantage, complexity, compatibility, trialability and observability) and their significance in the perception of academic staff at the RUB in relation to the probability of VLE adoption. These predictors are attributes of the VLE that determine the rate...... of adoption by various adopter group memberships (Innovators, Early Adopters, Early Majority, Late Majority, Laggards). Descriptive statistics and regression analysis were deployed to analyse adopter group memberships and predictor significance in VLE adoption and use. The results revealed varying attitudes...

  20. Intercountry versus Transracial Adoption: Analysis of Adoptive Parents' Motivations and Preferences in Adoption

    Science.gov (United States)

    Zhang, Yuanting; Lee, Gary R.

    2011-01-01

    The United States is one of the major baby-receiving countries in the world. Relatively little research has focused on why there is such a high demand for intercountry adoption. Using in-depth qualitative interviews with adoptive parents, the authors explored the reasons why Americans prefer to adopt foreign-born children instead of adopting…

  1. The Structure of Families who Adopt Children from Foster Care

    OpenAIRE

    Hansen, Mary Eschelbach

    2008-01-01

    The 1997 Adoption and Safe Families Act (ASFA) gave incentives to states to expedite the adoption of children from foster care. Administrative data describe the changes in adoptive families from 1996 to 2003 in terms of the marital status and sex of the household head and in terms of the relationship of the parents to the child prior to adoption. Patterns in the way children with special needs were matched with different kinds of families are described. The data show that agencies have tapped...

  2. Genetic Analysis of Two Female-Sterile Loci Affecting Eggshell Integrity and Embryonic Pattern Formation in Drosophila Melanogaster

    OpenAIRE

    Degelmann, A.; Hardy, P. A.; Mahowald, A. P.

    1990-01-01

    We have analyzed female-sterile mutations at the X-linked loci fs(1)Nas and fs(1)ph which show allele-specific effects on egg shell structure and embryonic pattern formation. The majority of mutant alleles at both loci lead to a collapsed egg phenotype. The maternal effect lethal phenotype is characterized by cuticle defects resembling those found in three autosomal mutants of the terminal class. We have analyzed the complementation behavior of various heteroallelic combinations at both loci ...

  3. Adoption: a forgotten paediatric speciality

    OpenAIRE

    Mather, M.

    1999-01-01

    OBJECTIVE—To study the medical, emotional, and developmental profile of children being considered for permanent substitute care.
DESIGN—A retrospective analysis of 100 adoption/permanency medicals.
SETTING—All children considered by the adoption and permanency panel in Greenwich, south east London, between 1994 and 1998.
OUTCOME MEASURES—Analysis of medical reports completed using the British Agencies for Adoption and Fostering form C (under 5 years) or form D (over 5 yea...

  4. Understanding the adoption of TELEs

    DEFF Research Database (Denmark)

    Christiansen, Ellen; Nyvang, Tom

    2006-01-01

    For a TELE to become adopted by the vast majority of individual learners as a tool for learning, adoption at the institutional level must be considered, because the vast majority of a population of technology users depends on external stimuli to adopt the technology. This article analyses a case...... a systemic, Bateson-inspired, understanding of learning and based on data from observation, qualitative interviews, and surveys, a number of barriers to adoption at the organisational level are identified, all requiring management initiatives if the application is to fully unfold its potential....

  5. Homosexuality and adoption in Brazil.

    Science.gov (United States)

    Uziel, A P

    2001-11-01

    Western societies are undergoing legal and policy changes in relation to laws governing the family, marital status, sexual orientation and the welfare of children, including in Brazil where, in the 1990s, the rights of homosexuals were incorporated into ongoing debates about what constitutes a family. This paper discusses the issue of adoption of children by homosexual men in Brazil, using information from court records from 1995-2000 in Rio de Janeiro, and from interviews with two judges, five psychologists and four social workers who evaluate those wishing to adopt. It uses the case records of one man's application to adopt, in which homosexuality became a central issue. Both the construction of masculinity in relation to parenting and concepts of the family were the parameters upon which the decision to allow him to adopt or not depended. Because the legislation does not specify what the sexual orientation of would-be adoptive parents should be, it is possible for single persons to adopt if they show they can be good parents. As more single people, alone or in couples, seek to adopt, it is important to clarify the criteria for judicial decisions on adoption applications. A dialogue is therefore needed on the meaning of family and whether and how it relates to sexual orientation. It is only on this basis that the courts can take a clear decision as to whether being homosexual is a relevant issue in regard to applications to adopt or not.

  6. Faculty Adoption of Educational Technology

    Science.gov (United States)

    Moser, Franziska Zellweger

    2007-01-01

    Although faculty support has been identified as a critical factor in the success of educational-technology programs, many people involved in such efforts underestimate the complexities of integrating technology into teaching. In this article, the author proposes an adoption cycle to help tackle the complex issue of technology adoption for…

  7. Healthy Travel for International Adoptions

    Centers for Disease Control (CDC) Podcasts

    2007-10-22

    The number of international adoptions, many from developing countries, has doubled in the last 10 years. This podcast discusses ways adoptive families can protect their own health and the health of their new children.  Created: 10/22/2007 by National Center for the Prevention, Detection and Control of Infectious Diseases (NCPDCID).   Date Released: 10/24/2007.

  8. Characteristics of adopted juvenile delinquents.

    Science.gov (United States)

    Kim, W J; Zrull, J P; Davenport, C W; Weaver, M

    1992-05-01

    There have been many reports describing the uniqueness of adopted children and adolescents' delinquent behaviors in terms of both their delinquent characteristics and courts' treatment of them. A total of 43 adopted juveniles, 32 extrafamilial (1.0%) and 11 intrafamilial (0.3%) adoptions were initially identified out of 3,280 juvenile delinquents. The adopted subjects were then compared with the demographically matched and offense matched nonadopted subjects. The family variables, such as marital and employment status of parents, were significantly different. However, there were only a few discernible trends, and in general there were no significant differences between the adopted and nonadopted juveniles in terms of their offense characteristics and dispositions. PMID:1592787

  9. Multimodal Resources in Transnational Adoption

    DEFF Research Database (Denmark)

    Raudaskoski, Pirkko Liisa

    The paper discusses an empirical analysis which highlights the multimodal nature of identity construction. A documentary on transnational adoption provides real life incidents as research material. The incidents involve (or from them emerge) various kinds of multimodal resources and participants...

  10. Technological adoption in health care

    OpenAIRE

    Pita Barros, Pedro; Martínez-Giralt, Xavier

    2010-01-01

    This paper addresses the impact of payment systems on the rate of technology adoption. We present a model where technological shift is driven by demand uncertainty, increased patients' benefit, financial variables, and the reimbursement system to providers. Two payment systems are studied: cost reimbursement and (two variants of) DRG. According to the system considered, adoption occurs either when patients' benefits are large enough or when the differential reimbursement across technologies o...

  11. Conservation Agriculture in Lesotho: Residue Use Patterns Among CA adopters vs. Non-Adopters

    OpenAIRE

    Wilcox, M.D.; Bisangwa, E.; Lambert, Dayton M.; Marake, Makoala V.; Walker, F.R.; Eash, Neal S.; Moore, Keith M.; Park, W M

    2012-01-01

    Recent efforts by the Government of Lesotho, non-government organizations (NGOs), and international attention have focused on developing conservation agriculture (CA) practices adapted to the cultural, economic, and agro-ecological conditions in Lesotho. Understanding the influence of the introduction of CA technologies on soil erosion, yields, labor allocation and gender roles is of critical importance for successfully deploying sustainable agriculture technologies.

  12. Adoption of Internet Shopping: Cultural Considerations in India and Australia

    OpenAIRE

    Sujana Adapa

    2008-01-01

    The current research paper examines the adoption of internet shopping patterns exhibited by Indian women currently residing in India and Australia emphasizing on the prevailing cultural dimensions. A conceptual framework has been developed based on the theoretical background which links intention to shop over internet and Hofstede’s cultural dimensions to adoption of internet shopping. In order to test the stated hypotheses, the proposed relationships between the varia...

  13. Adoption of managerial innovations: effect of adoption rationales on the adoption process

    OpenAIRE

    Daniel, Elizabeth Mary; Myers, Andrew; Dixon, Keith

    2008-01-01

    The aim of the research is to explore the complex phenomenon of the adoption of managerial innovations by organisations, with an attempt to identify relationships between various elements of this process. Four case studies were compiled using interview data from selected managers. The data provided a means of subjecting the rationales that Sturdy (2004) posited for the adoption of managerial innovations to empirical inquiry. The study also seeks to explore how the identified rationales may...

  14. Stuck in the Adoption Funnel: The Effect of Delays in the Adoption Process on Ultimate Adoption

    OpenAIRE

    Anja Lambrecht; Katja Seim; Catherine Tucker

    2007-01-01

    Online applications and services automate communications and transactions between firms and consumers, promising large efficiency gains. However, consumers have been slow to use these online technologies intensively, despite widespread adoption of the internet. Customers frequently undergo a staggered adoption process that may involve sign-up, experimentation, trial, and substantial usage until they fully embrace internet services. We ask whether delays in moving through the initial stages of...

  15. Comparison of CYP2D6 genotyping by allele-specific PCR with DXT phe-notype and gene chip testing%CYP2D6 PCR基因型与DXT表型和基因芯片检测的比较

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    目的:为了评价CYP2D6的基因型和表型的联系以及基因芯片在CYP2D6多基因分析中的应用.方法:242健康志愿者,口服dextromethorphan后收集尿液测定其代谢率,收集20ml血提取DNA,并通过基因特异性PCR和/(或)基因芯片分析CYP2D6*2--*11,*17和多拷贝CYP2D6基因,其中5个基因(*3、*4、*6、*7和*9)用PCR和CYD450基因芯片同时分析.结果:CYP2D6基因型比表型更富有信息和更能反映CYP2D6酶的表达.CYP2D6*3、*4、*6、*7和*9的基因检测在CYP450基因芯片和基因特异性PCR中显示高度的一致性.结论:基因芯片在检测基因多位点的多基因中是一个有发展前途和可靠的方法.%To evaluate association of genotype and phenotype of CYP2D6 and the application of oligonucleotide microarray hybridization genetic testing in CYP2D6 multiple alleles analyses. METHODS: Two hundred forty-two healthy volunteers were recruited, and a 60 mg oral dose of dextromethorphan (DXT) was administered to each for assessment of the DXT metabolic ratio [ MR]. A 20 ml blood sample was also collected for DNA isolation and testing. CYP2D6 alleles * 2-*11; * 17 and multiple CYP2D6 gene copies were tested by allele-specific PCR and/or the affymetrix CYP450 gene chip assay. Five of the CYP2D6 alleles ( * 3, * 4, *6, * 7, and * 9) were evaluated by both PCR and the CYP450 gene chip assay. RESULTS: The CYP2D6genotype was more informative and reflective in CYP2D6 enzyme expression than a phenotype. Genetic tests for the CYP2D6 * 3, * 4, * 6, * 7 and * 9 alleles showed a high degree of concordance between the CYP450 gene chip and AS-PCR methods. CONCLUSION: Oligonucleotide microarray hybridization is a promising and reliable approach for detecting multiple alleles at gene loci.

  16. Personality disorders in adopted versus non-adopted adults.

    Science.gov (United States)

    Westermeyer, Joseph; Yoon, Gihyun; Amundson, Carla; Warwick, Marion; Kuskowski, Michael A

    2015-04-30

    The goal of this epidemiological study was to investigate lifetime history and odds ratios of personality disorders in adopted and non-adopted adults using a nationally representative sample. Data, drawn from the National Epidemiological Survey on Alcohol and Related Conditions (NESARC), were compared in adopted (n=378) versus non-adopted (n=42,503) adults to estimate the odds of seven personality disorders using logistic regression analyses. The seven personality disorders were histrionic, antisocial, avoidant, paranoid, schizoid, obsessive-compulsive, and dependent personality disorder. Adoptees had a 1.81-fold increase in the odds of any personality disorder compared with non-adoptees. Adoptees had increased odds of histrionic, antisocial, avoidant, paranoid, schizoid, and obsessive-compulsive personality disorder compared with non-adoptees. Two risk factors associated with lifetime history of a personality disorder in adoptees compared to non-adoptees were (1) being in the age cohort 18-29 years (but no difference in the age 30-44 cohort), using the age 45 or older cohort as the reference and (2) having 12 years of education (but no difference in higher education groups), using the 0-11 years of education as the reference. These findings support the higher rates of personality disorders among adoptees compared to non-adoptees.

  17. Adopting to Agile Software Development

    Directory of Open Access Journals (Sweden)

    Linkevics Gusts

    2014-12-01

    Full Text Available Agile software development can be made successful, but there is no well-defined way how to achieve this. The problem is that the successful adoption of agile methods and practices is a complex process and this process should be customizable for a particular organization or a team. This research focuses on identification of agile methods and practices. Result of the research is the ranking of agile methods, practices and their usage trends. As some terms of agile software development are interpreted differently in different organizations and teams, terminology used is analyzed. Results of the research can be used as a reference material for those who are planning to adopt agile methods or are looking for the next agile practice to implement. Results will be used for the development of an expert system to support agile adoption.

  18. SMEs' E-commerce Adoption

    DEFF Research Database (Denmark)

    Scupola, Ada

    2009-01-01

    Purpose - Proposes providing an insight about factors affecting business-to-business e-commerce adoption and implementation in small to medium-sized enterprises (SMEs), highlighting similarities and differences between Danish and Australian SMEs.    Design/methodology/approach - The research...... is based on a wide literature review, focused on proposing a theoretical model of technological, environmental and organizational factors influencing e-commerce adoption and implementation. Subsequently, a questionnaire based on the research model has been developed and face-to-face interviews were...... conducted in Danish and Australian companies. Findings - The findings both corroborate previous results about significant factors affecting SMEs' b-to-b e-commerce adoption and implementation and provide new, interesting insights. The study also finds many similarities and differences between Denmark...

  19. Adopting Sustainability in the Organization

    DEFF Research Database (Denmark)

    Frandsen, Sanne; Morsing, Mette; Vallentin, Steen

    2013-01-01

    Purpose – The purpose of this paper is to explore the relationship between sustainability adoption and internal legitimacy construction. Design/methodology/approach – The paper is designed as a critical inquiry into existing research and practice on sustainability adoption, illustrated by two...... corporate vignettes. Findings – Prior studies tend to assume that awareness raising is a sufficient means to create employee commitment and support for corporate sustainability programs, while empirical observations indicate that managerial disregard of conflicting interpretations of sustainability may...... result in the illegitimacy of such programs. Originality/value – The authors suggest that a loosely coupled approach to sustainability adoption is a productive way to understand internal legitimacy construction, as it appreciates complexity and polyphony....

  20. Determinants of internet poker adoption.

    Science.gov (United States)

    Philander, Kahlil S; Abarbanel, B Lillian

    2014-09-01

    In nearly all jurisdictions, adoption of a new form of gambling has been a controversial and contentious subject. Online gambling has been no different, though there are many aspects that affect online gambling that do not appear in the brick and mortar environment. This study seeks to identify whether demographic, economic, political, technological, and/or sociological determinants contribute to online poker gambling adoption. A theoretical discussion of these categories' importance to online poker is provided and exploratory empirical analysis is used to examine their potential validity. The analysis revealed support for all of the proposed categories of variables thought to be predictive of online gambling legality. PMID:23661279

  1. Sharing Experiences of Intercountry Adoption

    DEFF Research Database (Denmark)

    Raudaskoski, Pirkko; McIlvenny, Paul

    of whom succeed in their 'quest' to adopt from abroad. One key feature of the series was the unintrusive use of consumer video cameras to record the emotionally charged moment when one or both of the partners receive news regarding their case over the telephone in their home (or workplace......). In this setting, the participants use language and other mediational means (eg. telephone, speakerphone, written details about the child, photograph, pen, paper) to accomplish the joint receipt of news about the child-to-be-adopted. In the analysis, we concentrate on how the parents’ affective displays...

  2. Has the Academy Adopted TQM?

    Science.gov (United States)

    Birnbaum, Robert; Deshotels, Judy

    1999-01-01

    A survey of 469 colleges and universities assessed the degree to which colleges and universities have adopted total quality management (TQM) or continuous quality improvement (CQI) techniques. Results suggest use of TQM/CQI is lower than predicted, at about 13% of institutions. Variations in extent of use of the approach are discussed. (MSE)

  3. ADOPT COMMUNICATIVE APPROACHES IN CET

    Institute of Scientific and Technical Information of China (English)

    LinSitan

    2004-01-01

    How to make CET tailored to the requirements of thesociety is a topic of great significance. This paper is meant toexplore the advantages of adopting communicative approaches inCET( College English Teaching ) by comparing communicativeapproaches with traditional ones and presenting our practice ofemploying communicative approaches as well as the result.

  4. Why Adoption of Standards Matters

    Science.gov (United States)

    Journal of Staff Development, 2016

    2016-01-01

    A total of 39 states have adopted, adapted, or endorsed the Standards for Professional Learning, including the standards issued in 2011 (labeled in red) and those published earlier (labeled in blue). Making a commitment to the standards is a commitment to continuous learning for all educators in a school.

  5. Internet Adoption: An Empirical Investigation

    Science.gov (United States)

    Ma, Junzhao

    2011-01-01

    The Internet has brought significant changes to the retail industry because it revolutionizes how information is transmitted and accessed. The main objective of this research is to enhance our understanding of people's adoption of the Internet and its implications for retail competition. This dissertation consists of two essays. The first essay…

  6. ADOPTATION OF INTERNET BANKING IN UAE: FACTORS UNDERLYING ADOPTION CHARACTERISTICS

    OpenAIRE

    Somkiat Mansumitrchai; Candy Chiu

    2012-01-01

    Technological development has provided tremendous benefits for many industries, including the banking business. The diffusion of internet banking has been witnessed in many countries worldwide. The United Arab Emirates (UAE) recently is one of the most significant economies in its region and in the global arena. Internet banking has been utilized for many years in UAE, and the number of internet banking adopters in UAE has increased steadily. The main focus of this paper was to identify the c...

  7. Adoption: Pediatric, Legislative and Social Issues

    OpenAIRE

    Davis, Joseph H.; Brown, Dirck W.

    1981-01-01

    Physicians may find themselves involved in many phases of the adoption process, ranging from advising infertile couples who wish to adopt a child to caring for adopted children, adolescents or adults. Recent legislation has been aimed at making it possible for children to be adopted who have been receiving foster care and at providing financial assistance to implement the adoption of children with handicaps and with medical problems. The adoption process is becoming more open. Adoptees are se...

  8. SMEs' E-commerce Adoption

    DEFF Research Database (Denmark)

    Scupola, Ada

    2009-01-01

    Purpose - Proposes providing an insight about factors affecting business-to-business e-commerce adoption and implementation in small to medium-sized enterprises (SMEs), highlighting similarities and differences between Danish and Australian SMEs.    Design/methodology/approach - The research is...... conducted in Danish and Australian companies. Findings - The findings both corroborate previous results about significant factors affecting SMEs' b-to-b e-commerce adoption and implementation and provide new, interesting insights. The study also finds many similarities and differences between Denmark and...... Australia.  Research limitations/implications - The main limitation relates to the difficulty of generalization of the findings to a larger population of SMEs.  To overcome this, a statistical survey is planned to be conducted in the future. Practical implications - The results of the empirical research...

  9. 等位基因特异扩增法研究中国人CYP2D6中速代谢的相关基因%ALLELE SPECIFIC AMPLIFICATON FOR CYP2D6 GENE RELATED TO INTERMEDIATE METABOLIZER IN CHINESE SUBJECTS

    Institute of Scientific and Technical Information of China (English)

    陈冰; 蔡卫民; 凌树森

    2001-01-01

    AIM To establish an allele specific PCR amplification (ASA-PCR) for determination of the genotype of CYP2D6*10B polymorphism in Chinese subjects. METHODS CYP2D6*10B alleles of 65 healthy Chinese subjects were analyzed by a two-step PCR assay and the correlation of genotype and phenotype was studied. RESULTS There were 20 CYP2D6*10B heterozygous genotypes subjects (wt/m) in 35 very extensive metabolizers (VEMs), which consisted the major part of VEM subjects (57%). Meanwhile, 20 subjects consisting 69% of 29 intermediate metabolizers were CYP2D6*10B homozygous mutant genotypes (m/m). The poor metabolizer was also m/m. The metabolic ratio of CYP2D6*10B m/m subjects were larger than wt/m and wild type, the values were -1.49±0.54, -2.20±0.49 and -2.47±0.61 (P<0.01). CONCLUSION PCR-ASA was shown to be a rapid and specific method. It can be used to study the genetic polymorphism, especially CYP2D6 intermediate metabolism.%目的 建立CYP2D6*10B的等位基因特异扩增法(ASA-PCR),以探讨中国人CYP2D6中速代谢的基因分型。方法 采用两步扩增法得到CYP2D6*10B等位基因特异片段,分析健康中国汉族人CYP2D6*10B等位基因,并探讨基因分型结果与右美沙芬表型分型结果的相关性。结果 35名表型为极快代谢受试者(VEMs)中,CYP2D6*10B以杂合子(wt/m)为主占57%;29名中速代谢受试者(IMs)以突变型纯合子(m/m)为主占69%;慢代谢受试者(PM)基因型为m/m。CYP2D6*10B m/m组的MR明显大于wt/m组和野生型组(wt/wt)。结论 ASA-PCR法有快速、准确的优点,可用于CYP2D6中速代谢的检测与研究。

  10. 竞争性等位基因特异性荧光探针聚合酶链法检测肺腺癌组织 EGFR 基因突变%Detection of epidermal growth factor receptor mutations in lung adenocarcinoma tissues by the method of competitive allele - specific TaqMan PCR

    Institute of Scientific and Technical Information of China (English)

    孟谊; 禹立霞; 杨杨; 王立峰

    2016-01-01

    Objective:To explore the value of detecting mutations on epidermal growth factor receptor(EGFR) gene in treatment - naive adenocarcinoma tissue by competitive allele - specific TaqMan PCR(castPCR). And we also investigated the correlation between EGFR mutation status and their clinical characteristics. Methods:We col-lected 103 lung adenocarcinoma patients' clinical pathologic data,formalin - fixed paraffin - embedded(FFPE)tis-sues in The Affiliated Drum Tower Hospital of Medical School of Nanjing University from November 2010 to December 2014. The castPCR method was employed to detect the EGFR mutation statues(exon 19,2235 - 2249 and 2236 -2249,exon 20 T790M,exon 21 L858R)of 103 treatment - naive FFPE tissue samples. Results:Of 103 lung adeno-carcinoma patients 54 were detected with at least one site of EGFR mutations(mutation rate 52. 43% ). There were 45(43. 69% )patients carrying sensitive mutation(exon 19 and/ or exon 21)and 11 people(10. 68% )carrying ex-on 20 mutation. Some patients harbored complex mutations:2 carried exon 19 and 20 mutations,4 carried exon 19 and 21 mutations,and 1 carried exon 20 and 21 mutation. Conclusion:We have demonstrated the clinical feasibility of uti-lizing the castPCR method to detect the EGFR mutation statues from lung cancer tissue.%目的:探讨竞争性等位基因特异性荧光探针聚合酶链(castPCR)法检测初治肺腺癌组织表皮生长因子受体(EGFR)基因突变的应用价值,及 EGFR 突变状态与患者临床生物学行为的相关性。方法:收集2010年10月至2014年12月南京鼓楼医院103例初治肺腺癌组织标本及患者的临床病理资料。使用 castPCR 法检测 EGFR 基因突变(外显子19 del 2235-2249和 del 2236-2250、外显子20 T790M、外显子21 L858R)。结果:103例肺腺癌患者肿瘤组织中共有54例检出了存在至少一个位点的 EGFR 基因突变(突变率52.43%),其中敏感突变(外显子19和/或外显子21)45人(43.69%

  11. Amplificação gênica alelo-específica na caracterização das hemoglobinas S, C e D e as interações entre elas e talassemias beta Allele-specific genic amplification in the characterization of hemoglobins S, C, D and interactions among them and with beta thalassemia

    Directory of Open Access Journals (Sweden)

    Luciane Cristina Bertholo

    2006-08-01

    possible interactions, based on the allele-specific genic amplification (PCR-AE with the use in parallel two primers that differ at their 3’ extremities and are complementary to the normal or mutated sequences. RESULTS AND DISCUSSION: The results make evident the validation of this methodology in the characterization of these mutations, once this procedure is easy to execute,to reproduce, as well as it is possible to be applied to a significative number of samples.

  12. International Adoption: The Child's Story

    OpenAIRE

    Bartholet, Elizabeth

    2008-01-01

    Millions of infants and young children worldwide are desperately in need of nurturing homes. Many are living in institutions, and many on the streets, and almost all these children will either die in these situations, or if they survive, will emerge into adulthood so damaged by their childhood experience, and so deprived of parenting, educational and other essential childhood opportunities, that they will be unable to function in the worlds of family and work. International adoption could pro...

  13. Revision of the Adoption Law

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    DECEMBER 29, 1991, saw thePresident of the People’sRepublic of China promulgatea new adoption law by Order 54, a lawwhich was introduced to the seventhNational People’s Congress at thetwenty-third meeting of its StandingCommittee and which took effect fromApril 1, 1992. This was the first suchadoption law in China and, althoughadoption did occur prior to itsimplementation, it was not then boundby legal measures or guarantee.

  14. Adoption in CZE Legal System

    OpenAIRE

    Mikolášková, Lucie

    2011-01-01

    This bachelor thesis seeks to characterize substitute family care, alternative care in institutions and educational institution that help children in need. It proides the basic legislation that is important to substitute family care, institucial care and child protection. Substitute family care includes adoption, foster care, guardianship and custody. Under the care in institutions falls education in infant institutions, in children´s homes, in diagnostic anad educational institutions, in soc...

  15. Adopting to Agile Software Development

    OpenAIRE

    Linkevics Gusts

    2014-01-01

    Agile software development can be made successful, but there is no well-defined way how to achieve this. The problem is that the successful adoption of agile methods and practices is a complex process and this process should be customizable for a particular organization or a team. This research focuses on identification of agile methods and practices. Result of the research is the ranking of agile methods, practices and their usage trends. As some terms of agile software development are inter...

  16. Social Capital Framework in the Adoption of E-Learning

    Science.gov (United States)

    Barton, Siew Mee

    2013-01-01

    This is a study of the influence of social and cultural factors on the adoption of e-learning in higher education in Malaysia, Indonesia, Turkey, Singapore and Australia. Particular attention in each case was given to factors relating to social capital, attitudes and patterns of behavior in leadership, entrepreneurialism, and teaching and to…

  17. Factors Affecting Mobile Banking Adoption in the United States

    Science.gov (United States)

    Engwanda, Michel Ndongola

    2014-01-01

    Mobile banking (m-banking) allows consumers to conduct financial transactions without temporal and spatial constraints through Internet-enabled mobile devices such as smartphones. The adoption patterns are of particular research interest because m-banking penetration has been relatively low even though smartphones are the most dominant forms of…

  18. IDT-TAM Integrated Model for IT Adoption

    Institute of Scientific and Technical Information of China (English)

    ZHANG Nan; GUO Xunhua; CHEN Guoqing

    2008-01-01

    Two classical theories, the technology acceptance model (TAM) and the innovation diffusion theory (IDT), were integrated into a model for analyzing individual information technology (IT) adoption behavior. In this IDT-TAM framework, the perceived characteristics of innovation are categorized as subjective evaluation, objective conditions, and interaction factors. The cognitive mechanisms that drive user IT acceptance are analyzed based on the patterns through which these characteristics influence individual user IT adoption behavior. The model was tested using an empirical survey regarding the adoption and use of e-mail in China. Results from a structural equation model analysis illustrate that the model provides meaningful insights for understanding, explaining, and predicting the IT adoption behavior of Chinese users.

  19. Meeting the adoption support needs of adopted adults who have been abused in their adoptive family: Lessons from historical placements

    OpenAIRE

    Harris, Perlita

    2014-01-01

    This article focuses on a group whose voice is rarely heard: adopted adults who have been abused or neglected within their adoptive family. The findings are drawn from a larger study of post-adoption services and suggest that the abuse and neglect of children by adoptive family members may be more common historically than has been hitherto acknowledged. The article considers this finding in the context of the changes that have occurred in adoption legislation, policy and practice since these ...

  20. 18 CFR 341.6 - Adoption rule.

    Science.gov (United States)

    2010-04-01

    ... 18 Conservation of Power and Water Resources 1 2010-04-01 2010-04-01 false Adoption rule. 341.6... SUBJECT TO SECTION 6 OF THE INTERSTATE COMMERCE ACT § 341.6 Adoption rule. (a) Change in name of carrier... such occurrence. The filing of adoption notices and adoption supplements requires no notice period....

  1. 33 CFR 230.21 - Adoption.

    Science.gov (United States)

    2010-07-01

    ... FOR IMPLEMENTING NEPA § 230.21 Adoption. See 40 CFR 1506.3. A district commander will normally adopt... recirculated as provided in 40 CFR 1506.3 (b) or (c), the adopted EIS with the supplement, if any, will be... 33 Navigation and Navigable Waters 3 2010-07-01 2010-07-01 false Adoption. 230.21 Section...

  2. Policy Issues in Gay and Lesbian Adoption.

    Science.gov (United States)

    Sullivan, Ann

    1995-01-01

    Notes that adoption agencies have developed few specific policies on the issue of lesbian and gay adoption. Provides an overview of key considerations about homosexual adopters, including beliefs and values of agency professionals, the legal and social ramifications of adoption into a relationship not based on marriage, and possible consequences…

  3. Adopted: A practical salinity scale

    Science.gov (United States)

    The Unesco/ICES/SCOR/IAPSO Joint Panel on Oceanographic Tables and Standards has recommended the adoption of a Practical Salinity Scale, 1978, and a corresponding new International Equation of State of Seawater, 1980. A full account of the research leading to their recommendation is available in the series Unesco Technical Papers in Marine Science.The parent organizations have accepted the panel's recommendations and have set January 1, 1982, as the date when the new procedures, formulae, and tables should replace those now in use.

  4. Adoption of Mobile Payment Platforms

    DEFF Research Database (Denmark)

    Staykova, Kalina Stefanova; Damsgaard, Jan

    2016-01-01

    Numerous mobile payment solutions, which rely on new disruptive technologies, have been launched on the payment market in recent years. But despite the growing number of mobile payment apps, very few solutions have turned to be successful as the majority of them fail to gain a critical mass...... of users. In this paper, we investigate successful platform adoption strategies by using the Reach and Range Framework for Multi-Sided Platforms as a strategic tool to which mobile payment providers can adhere in order to tackle some of the main challenges they face throughout the evolution...

  5. Juridical aspects of post mortem adoption

    OpenAIRE

    Sergio Rodrigo Martinez; Natália Novais Fernandes Gomes

    2015-01-01

    The legislature, with the enactment of the Statute of Children and Adolescents, now provides the named posthumous adoption, allowing the death of the adopter, in the course of the procedure does not generate the extinction pleaded adoption. Has the purpose to show that, notwithstanding the absence of such law, the construction of new adopters and adopted by families are recognized, since permeated the socially demonstrated affection, even in passing the death of the person occurs before you h...

  6. [Adoptive parents' satisfaction with the adoption experience and with its impact on family life].

    Science.gov (United States)

    Sánchez-Sandoval, Yolanda

    2011-11-01

    In this study, we discuss the relevance of adoptive families' satisfaction in the assessment of adoption processes. The effects of adoption on a sample group of 272 adoptive families are analyzed. Most families show high levels of satisfaction as to: their decision to adopt, the features of their adopted children and how adoption has affected them as individuals and as a family. Statistical analyses show that these families can have different satisfaction levels depending on certain features of the adoptees, of the adoptive families or of their educational style. Life satisfaction of the adoptees is also related to how their adoptive parents evaluate the adoption. PMID:22047850

  7. [Adoptive parents' satisfaction with the adoption experience and with its impact on family life].

    Science.gov (United States)

    Sánchez-Sandoval, Yolanda

    2011-11-01

    In this study, we discuss the relevance of adoptive families' satisfaction in the assessment of adoption processes. The effects of adoption on a sample group of 272 adoptive families are analyzed. Most families show high levels of satisfaction as to: their decision to adopt, the features of their adopted children and how adoption has affected them as individuals and as a family. Statistical analyses show that these families can have different satisfaction levels depending on certain features of the adoptees, of the adoptive families or of their educational style. Life satisfaction of the adoptees is also related to how their adoptive parents evaluate the adoption.

  8. Electronic Dental Records System Adoption.

    Science.gov (United States)

    Abramovicz-Finkelsztain, Renata; Barsottini, Claudia G N; Marin, Heimar Fatima

    2015-01-01

    The use of Electronic Dental Records (EDRs) and management software has become more frequent, following the increase in prevelance of new technologies and computers in dental offices. The purpose of this study is to identify and evaluate the use of EDRs by the dental community in the São Paulo city area. A quantitative case study was performed using a survey on the phone. A total of 54 offices were contacted and only one declinedparticipation in this study. Only one office did not have a computer. EDRs were used in 28 offices and only four were paperless. The lack of studies in this area suggests the need for more usability and implementation studies on EDRs so that we can improve EDR adoption by the dental community.

  9. Innovation and Adoption of Electronic Business Technologies

    OpenAIRE

    Sülzle, Kai

    2007-01-01

    This paper presents a duopoly model of e-business technology adoption. A leader and a follower benefit from a new e-business technology with uncertain quality depending on its innovation and adoption cost and both firms’ adoption timing. When innovation and adoption require large set-up costs, the leader favors quick adoption by the follower. The follower prefers either late or no adoption. This is due to a delayed first-mover benefit which stems from an innovators’ capability to impose a new...

  10. Adoption of Children with Disabilities: An Exploration of the Issues for Adoptive Families

    Science.gov (United States)

    Good, Gretchen A.

    2016-01-01

    This systematic literature review is an exploration of issues for adoptive families throughout the adoption process and into the various phases of the life of the adoptive family. Although there has been much recent research related to adoption, in general, very little adoption literature addresses the often unspoken needs of families who want to…

  11. National Foster Care and Adoption Directory Search

    Science.gov (United States)

    ... Directory National Foster Care & Adoption Directory App State Guides & Manuals State Related Organizations Lists Adoption Assistance by State Child Welfare Workload Compendium More Tools & Resources Logic Model Builders Related Organizations Learning Center ...

  12. Innovation Type, Radicalness, and the Adoption Process.

    Science.gov (United States)

    Damanpour, Fariborz

    1988-01-01

    Reviews studies on the impact of organizational factors on the adoption of innovations along three dimensions (innovation type, innovation radicalness, and stages of adoption), finding considerable agreement. Proposes a research agenda for future studies. (SR)

  13. Macro influencers of electronic health records adoption.

    Science.gov (United States)

    Raghavan, Vijay V; Chinta, Ravi; Zhirkin, Nikita

    2015-01-01

    While adoption rates for electronic health records (EHRs) have improved, the reasons for significant geographical differences in EHR adoption within the USA have remained unclear. To understand the reasons for these variations across states, we have compiled from secondary sources a profile of different states within the USA, based on macroeconomic and macro health-environment factors. Regression analyses were performed using these indicator factors on EHR adoption. The results showed that internet usage and literacy are significantly associated with certain measures of EHR adoption. Income level was not significantly associated with EHR adoption. Per capita patient days (a proxy for healthcare need intensity within a state) is negatively correlated with EHR adoption rate. Health insurance coverage is positively correlated with EHR adoption rate. Older physicians (>60 years) tend to adopt EHR systems less than their younger counterparts. These findings have policy implications on formulating regionally focused incentive programs.

  14. Developmental Outcomes of Internationally Adopted Children

    OpenAIRE

    Welsh, Janet A.; Viana, Andres G.

    2012-01-01

    This study followed the development of a sample of 106 (67 girls) internationally adopted children over a period of 18 months. Children were adopted from five birth regions, including China, Korea, Latin America, Eastern Europe, and other Asian countries. Mean age at adoption was 11 months. Mothers completed the Ages and Stages Questionnaire (ASQ) at 6, 12, and 24 months post-adoption, assessing children's gross and fine motor, communicative, personal-social, and problem solving skills. Resul...

  15. Globalization and international adoption from China

    OpenAIRE

    Dowling, Monica; Brown, Gill

    2009-01-01

    Since the mid-1990s, China has become one of the major countries from which children are adopted overseas. This paper examines ways in which globalization has contributed to the development of international adoption from China and explores cultural and historical attitudes to population growth, child abandonment and adoption. How China's social, economic and welfare policies have affected adoption policies and practices are discussed, with reference to ethnographic fieldwork undertaken by the...

  16. Business model elements impacting cloud computing adoption

    DEFF Research Database (Denmark)

    Bogataj, Kristina; Pucihar, Andreja; Sudzina, Frantisek

    The paper presents a proposed research framework for identification of business model elements impacting Cloud Computing Adoption. We provide a definition of main Cloud Computing characteristics, discuss previous findings on factors impacting Cloud Computing Adoption, and investigate technology...... adoption theories, such as Diffusion of Innovations, Technology Acceptance Model, Unified Theory of Acceptance and Use of Technology. Further on, at research model for identification of Cloud Computing Adoption factors from a business model perspective is presented. The following business model building...

  17. Precision Agriculture Technology Adoption for Cotton Production

    OpenAIRE

    Paxton, Kenneth W.; Mishra, Ashok K.; Chintawar, Sachin; Larson, James A.; Roberts, Roland K.; English, Burton C.; Lambert, Dayton M.; Marra, Michele C.; Larkin, Sherry L.; Reeves, Jeanne M.; Martin, Steven W

    2010-01-01

    Many studies on the adoption of precision technologies have generally used logit models to explain the adoption behavior of individuals. This study investigates factors affecting the number of specific types of precision agriculture technologies adopted by cotton farmers. Particular attention is given to the influence of spatial yield variability on the number of precision farming technologies adopted, using a Count data estimation procedure and farm-level data. Results indicate that farmers ...

  18. Adoption of mobile commerce : an exploratory analysis

    OpenAIRE

    Pedersen, Per E.

    2001-01-01

    Even though the literature on the adoption and use of mobile services is quite extensive, surprisingly few studies are found applying traditional models of ICT adoption such as the technology acceptance model. With the introduction of 3G services, a convergence of mobile services and traditional Internet services is expected. Thus, traditional models of ICT adoption may be applied improving our understanding of the adoption of mobile services. In this report, we review relevant studies in ado...

  19. Adoption of Internet Strategies by Agribusiness Firms

    OpenAIRE

    Henderson, Jason R.; Dooley, Frank J.; Akridge, Jay T.; Carerre, Antonio

    2005-01-01

    This paper explores the factors guiding Internet adoption by agribusiness firms. The relationship between Internet strategies and manager perceptions on the barriers to and catalysts for Internet adoption are analyzed in a supply-chain management framework. Using factor analysis and an ordered Probit model, results indicate that Internet strategies are more likely to be adopted in larger firms with a global scope. Also, manager perceptions regarding the impact of Internet adoption on transact...

  20. United Kingdom adoption agency Web sites

    OpenAIRE

    Fenton, Roger

    2004-01-01

    The United Kingdom made major changes in its law on child adoption in 2002. The government is also requiring central and local government agencies to provide more information to the public via the Internet. This paper investigates how U.K. local authority and voluntary adoption agencies present information about themselves and adoption on the Internet. It examines the Web sites of 30 U.K. adoption agencies in terms of 189 features of information architecture and information content, to see to...

  1. Conceptualization of Electronic Government Adoption

    Directory of Open Access Journals (Sweden)

    Rahmath Safeena

    2013-03-01

    Full Text Available E-government facilitates provision of relevant government information in electronic form to the citizens ina timely manner and better service delivery to citizens. Information Technology today is recognized as aneffective tool for turning the economic activity in efficient governance and in developing human resource.E-government is a kind of governmental administration which is based on ICT Services. The essence of egovernmentis using information technology to break the boundary of administrative organizations, andbuild up a virtual electronic government. E-government initiatives are common in most countries as theypromise a transparent, citizen-centric government and reduce operational cost. Emerging with Egovernment,theories and practices of public administration have stepped into a new knowledge era. Egovernmentpresents a tremendous impetus to move forward with higher quality, cost-effective, governmentservices and a better relationship between citizens and government. This paper discusses the differentissues, challenges, adoption factors for e-government implementation, conceptual demarcation on thesefactors and, presents a conceptual framework for better e-government performance.

  2. 25 CFR 75.18 - Adoption.

    Science.gov (United States)

    2010-04-01

    ... 25 Indians 1 2010-04-01 2010-04-01 false Adoption. 75.18 Section 75.18 Indians BUREAU OF INDIAN... OF CHEROKEE INDIANS, NORTH CAROLINA § 75.18 Adoption. The Tribal Council of the Eastern Band of Cherokee Indians shall be empowered to enact ordinances governing the adoption of new members....

  3. 40 CFR 1506.3 - Adoption.

    Science.gov (United States)

    2010-07-01

    ... 40 Protection of Environment 32 2010-07-01 2010-07-01 false Adoption. 1506.3 Section 1506.3 Protection of Environment COUNCIL ON ENVIRONMENTAL QUALITY OTHER REQUIREMENTS OF NEPA § 1506.3 Adoption. (a) An agency may adopt a Federal draft or final environmental impact statement or portion...

  4. 14 CFR 221.160 - Adoption notice.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Adoption notice. 221.160 Section 221.160... REGULATIONS TARIFFS Adoption Publications Required To Show Change in Carrier's Name or Transfer of Operating Control § 221.160 Adoption notice. (a) When the name of a carrier is changed or when its operating...

  5. 47 CFR 61.171 - Adoption notice.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 3 2010-10-01 2010-10-01 false Adoption notice. 61.171 Section 61.171 Telecommunication FEDERAL COMMUNICATIONS COMMISSION (CONTINUED) COMMON CARRIER SERVICES (CONTINUED) TARIFFS Adoption of Tariffs and Other Documents of Predecessor Carriers § 61.171 Adoption notice. When a...

  6. Adoption of mobile services : moderating effects of service’s information intensity

    OpenAIRE

    Sendecka, Lenka

    2006-01-01

    The increased use of mobile technology has been dramatic in the recent years. Understanding some of the reasons for this immense expansion lies in comprehending the factors behind consumer usage patterns. Hence it is becoming more and more crucial for today’s marketers to study how and why consumers adopt (new) technology and mobile services. The knowledge of adoption process and the factors driving it may be relevant for both providers and customers. Several models for adoption of informatio...

  7. Heteronormative consensus in the Norwegian same-sex adoption debate?

    Science.gov (United States)

    Anderssen, Norman; Hellesund, Tone

    2009-01-01

    This article investigates the Norwegian newspaper debate (1998-2002) on the right of homosexual couples to adopt children. It identifies two patterns of meaning within which both anti-adoption and pro-adoption sides of the debate were located: 1) the nuclear family as reference point; and 2) a focus on innate qualities. Parallell to a continuous liberalization of sexualities in Norway we seem to witness a consensus on heteronormativity in Norway on both sides of the debate as the basic axiom in public discussions on homosexuality and adoption. In this article, we explore the nature of the heteronormative arguments and the reason for their appearance in this particular debate. The two patterns of meaning reproduce a perception of lesbians and gays as either a worthy or unworthy minority. These findings may be seen as reflecting fundamental positions regarding the Norwegian modernization project, where both sides of the debate see homosexuality as a central symbol. State feminism may also have played the role of reinforcing gender categories and thereby served as an important condition of possibility for contemporary heteronormativity.

  8. Heteronormative consensus in the Norwegian same-sex adoption debate?

    Science.gov (United States)

    Anderssen, Norman; Hellesund, Tone

    2009-01-01

    This article investigates the Norwegian newspaper debate (1998-2002) on the right of homosexual couples to adopt children. It identifies two patterns of meaning within which both anti-adoption and pro-adoption sides of the debate were located: 1) the nuclear family as reference point; and 2) a focus on innate qualities. Parallell to a continuous liberalization of sexualities in Norway we seem to witness a consensus on heteronormativity in Norway on both sides of the debate as the basic axiom in public discussions on homosexuality and adoption. In this article, we explore the nature of the heteronormative arguments and the reason for their appearance in this particular debate. The two patterns of meaning reproduce a perception of lesbians and gays as either a worthy or unworthy minority. These findings may be seen as reflecting fundamental positions regarding the Norwegian modernization project, where both sides of the debate see homosexuality as a central symbol. State feminism may also have played the role of reinforcing gender categories and thereby served as an important condition of possibility for contemporary heteronormativity. PMID:19197645

  9. The debbuggable interpreter design pattern

    OpenAIRE

    Vrany, Jan; Bergel, Alexandre

    2007-01-01

    The use of Interpreter and Visitor design patterns has been widely adopted to implement programming language interpreters due to their expressive and simple design. However, no general approach to conceive a debugger is commonly adopted. This paper presents the debuggable interpreter design pattern as a general approach to extend a language interpreter with debugging facilities such as step-over and step-into. Moreover, it enables multiple debuggers coexisting and extends the Interpreter a...

  10. Simultaneous Versus Sequential Complementarity in the Adoption of Technological and Organizational Innovations

    DEFF Research Database (Denmark)

    Battisti, Giuliana; Rabbiosi, Larissa; Colombo, Massimo G.

    2015-01-01

    It is generally suggested that technological and organizational innovations, being complementary, need to be adopted simultaneously. Nevertheless, sequential rather than simultaneous adoption of these two types of innovation may be optimal. In this paper, we analyze the pattern of mutual causation...... of technological and organizational innovations and contribute to the understanding of their interdependencies......

  11. Inter-Organizational Information Systems Adoption

    DEFF Research Database (Denmark)

    Lyytinen, Kalle; Damsgaard, Jan

    2011-01-01

    In this article we propose a new complementary approach to investigate Inter-Organizational Information Systems (IOIS) adoption called configuration analysis. We motivate the need for a new approach by the common observation that the structure and the strategy of an IOIS are interdependent...... propose that IOIS scholars need to look beyond the single adopting organization in IOIS adoption studies and in contrast consider adoption units what we call an adoption configuration. Each such configuration can be further characterized along the following dimensions: (1) vision, (2) key functionality...

  12. Adoptive immunotherapy of advanced melanoma.

    Science.gov (United States)

    Shapira-Frommer, Ronnie; Schachter, Jacob

    2012-09-01

    Adoptive cell therapy (ACT) has emerged as an effective therapy for patients with metastatic melanoma. Since the first introduction of the protocol in 1988 [1], major improvements have been achieved with response rates of 40%-72% among patients who were resistant to previous treatment lines. Both cell product and conditioning regimen are major determinants of treatment efficacy; therefore, developing ACT protocols explore diverse ways to establish autologous intra-tumoral lymphocyte cultures or peripheral effector cells as well as different lymphodepleting regimens. While a proof of feasibility and a proof of concept had been established with previous published results, ACT will need to move beyond single-center experiences, to confirmatory, multi-center studies. If ACT is to move into widespread practice, it will be necessary to develop reproducible high quality cell production methods and accepted lymphodepleting regimen. Two new drugs, ipilimumab (Yervoy, Bristol-Myers Squibb) and vemurafenib (Zelboraf, Roche), were approved in 2011 for the treatment of metastatic melanoma based on positive phase III trials. Both drugs show a clear overall survival benefit, so the timing of when to use ACT will need to be carefully thought out. In contrast to these 2 new, commercially available outpatient treatments, ACT is a personally-specified product and labor-intensive therapy that demands both acquisition of high standard laboratory procedures and close clinical inpatient monitoring during treatment. It is unique among other anti-melanoma treatments, providing the potential for a durable response following a single, self-limited treatment. This perspective drives the efforts to make this protocol accessible for more patients and to explore modifications that may optimize treatment results.

  13. Adoption: a new Stata routine for estimating consistently population technological adoption parameters

    OpenAIRE

    Aliou Diagne

    2012-01-01

    In Agricultural Economics 37 (2007) 201–210, using a counterfactual outcomes framework, Diagne and Demont showed that observed sample technological adoption rate does not consistently estimate the population adoption rate even if the sample is random. Likewise, it is shown that a model of adoption with observed adoption outcome as a dependent variable and where exposure to the technology is not observed and controlled for cannot yield consistent estimates of the determinants of adoption. In...

  14. Comparisons of Adopted and Non-Adopted Young Adults' Heterosexual Relationships

    OpenAIRE

    Winward, Bryan W.

    2005-01-01

    Adoption has been seen as a good solution for providing each party involved in the traditional adoption (i.e. , young birth parents, infants, and infertile couples) with a positive outcome. Adoptions, however, are generally more complex and diverse than supposed. Variables such as age of placement, levels of abuse and neglect, and foster care have been shown to place adopted children at risk for later struggles and challenges. Research studying outcomes of adopted young adults and their forma...

  15. 'I started to feel not ashamed of who I was': Transracially adopted adults and adoption support

    OpenAIRE

    Harris, Perlita

    2014-01-01

    Transracially adopted adults are largely invisible as users of adoption support services in England and their voices are rarely heard in discussions about appropriate provision. This article reports findings from interviews with transracially adopted adults and transracially adopted adult participants at a lesbian, gay and bisexual focus group, carried out as part of a mixed-method study of service users’ views and experiences of post-adoption services delivered by an independent agency. The ...

  16. On Vertical Relations and Technology Adoption Timing

    OpenAIRE

    Maria Alipranti; Chrysovalantou Miliou; Emmanuel Petrakis

    2014-01-01

    This paper explores how vertical relations influence the timing of new technology adoption. It shows that both the bargaining power distribution among the vertically related firms and the contract type through which vertical trading is conducted affect crucially the speed of adoption: the downstream firms can adopt later a new technology when the upstream bargaining power increases as well as when wholesale price contracts, instead of two-part tariffs, are employed. Importantly, it shows that...

  17. Trade Liberalization, Democratization and Technology Adoption

    OpenAIRE

    Cervellati, Matteo; Naghavi, Alireza; Toubal, Farid

    2014-01-01

    We study the role of trade liberalization, democratization and their interaction for technology adoption. A general equilibrium theory with heterogeneous skills predicts a complementarity between trade and political regimes. Openness should accelerate technology adoption if coupled with democratization but may lead to a slow down if these regime changes are imbalanced. We use panel data on technology adoption at the sectoral level for the period 1980-2000 by exploiting within country variatio...

  18. Adoption of agile software development in Vietnam

    OpenAIRE

    Tran, Trung Hieu; Duong, Nhat Duy

    2014-01-01

    Agile software development method is considered as an essential for software companies, bringing critical benefits. The adoption and implementation of agile software development method enable organizations to adapt to the dramatically changing business environment. This thesis aims at investigating the adoption practices of agile development methods in companies in Vietnam, with three main goals. The first one is to explore the reasons and motivations determining the adoption of agile dev...

  19. How to Adopt a Child from China

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Foreigners who want to adopt a child from China should present the documents listed below, through their government or an adoption organization entrusted by their government, to a Chinese government empowered adoption organization. These documents should be notarized by the foreign affairs office of their country, or an organization it so empowers, as well as by the Chinese embassy or consulate in their country. These documents include:

  20. ICT Adoption in Facilities Management Supply Chain

    DEFF Research Database (Denmark)

    Scupola, Ada

    2012-01-01

    This article involves a qualitative study of factors impacting the adoption of ICT solutions in the Danish facility management supply chain. The results show that there are a number of drivers and barriers that influence the adoption of ICT solutions in this service sector. These have been grouped...... concerned with ICT adoption, operations and service management (especially facilities management) as well as operation managers and ICT managers....

  1. The Profiling the Internet Banking Adopter

    Directory of Open Access Journals (Sweden)

    Sanmugam, A

    2005-01-01

    Full Text Available This paper presents the profile of the internet banking adopter in Malaysia based on a large scale survey. A logistic model is used to estimate the probability of a bank customer adopting internet banking. The profile of the adopter is constructed using demographic, social economic and technological capacity indicators. The method is a very simple one, which can be used by financial institutions to gain better understanding of their own internet banking customers.

  2. ADOPTION OF PRECISION AGRICULTURE TECHNOLOGY IN MISSISSIPPI

    OpenAIRE

    Hudson, Darren; Hite, Diane

    2001-01-01

    Precision application technology has been an important topic in agriculture in recent years. This technology has the promise to improve farm management through improved information and control over in-field variability of soil characteristics and productivity. Despite this apparent promise, recent studies have shown that adoption has been low. However, little is known about the adoption of this technology in Mississippi or the reasons for or against adoption as seen through the eyes of the pr...

  3. Reasons for Attrition Among Public Adoption Seekers

    OpenAIRE

    Weissinger, Erika

    2013-01-01

    The purpose of this study is to identify the main reasons public adoption seekers do not complete the adoption process. I conduct interviews with public adoption seekers from two California counties who dropped out of the process. I find that the most common reasons given for dropping out of the process are -changes in personal circumstance (for reasons such as losing a job or becoming ill); -dissatisfaction with agencies (for reasons such as poor customer service, the process being too len...

  4. The geography of Internet adoption by retailers

    OpenAIRE

    Weltevreden, J.W.J.; Atzema, O.A.L.C.; Frenken, K.; De Kruif, K.; Oort, F.G. van

    2005-01-01

    Up till now, the literature on Internet adoption by retailers paid little attention to spatial variables. Using data on 27,000 retail outlets in the Netherlands, we investigate the geographical diffusion of Internet adoption by Dutch retailers. More precise, we examine to what extent retail Internet adoption differs between shopping centers, cities, and regions, while controlling for product and organizational variables. Results of the linear and multinomial logistic regressions suggest that ...

  5. Determinants and Outcomes of Internet Banking Adoption

    OpenAIRE

    Mei Xue; Lorin M. Hitt; Pei-yu Chen

    2011-01-01

    This paper examines the drivers of adoption of Internet banking and the linkages among adoption drivers and outcomes (product acquisition, service activity, profitability, loyalty). We relate Internet banking adoption to customer demand for banking services, the availability of alternative channels, customers' efficiency in service coproduction ("customer efficiency"), and local Internet banking penetration. We find that customers who have greater transaction demand and higher efficiency, and...

  6. Measures for Predictors of Innovation Adoption.

    Science.gov (United States)

    Chor, Ka Ho Brian; Wisdom, Jennifer P; Olin, Su-Chin Serene; Hoagwood, Kimberly E; Horwitz, Sarah M

    2015-09-01

    Building on a narrative synthesis of adoption theories by Wisdom et al. (2013), this review identifies 118 measures associated with the 27 adoption predictors in the synthesis. The distribution of measures is uneven across the predictors and predictors vary in modifiability. Multiple dimensions and definitions of predictors further complicate measurement efforts. For state policymakers and researchers, more effective and integrated measurement can advance the adoption of complex innovations such as evidence-based practices. PMID:24740175

  7. Corporate Social Responsibility in Danish International Adoption

    OpenAIRE

    Ramsøe, Lukas; Axelsen, William Nordahl; Rasmussen, Ida-Maria Feldt; Hohle, Camilla Victoria

    2015-01-01

    This project examines the term ​Corporate Social Responsibility ​(CSR) in proportion to the adoption organization ​Danish International Adoption​. The project wish to seek a higher understanding of how the organization uses this term, after a lot of criticism about adoption has occurred in the media. Furthermore this project will focus on the development of the organization when the critic in the media addresses a picture that Danish International Adoption does not take this term into acco...

  8. Adoptive immunotherapy for cancer: building on success

    OpenAIRE

    Gattinoni, Luca; Powell, Daniel J.; Rosenberg, Steven A.; Restifo, Nicholas P

    2006-01-01

    Adoptive cell transfer after host preconditioning by lymphodepletion represents an important advance in cancer immunotherapy. Here, we describe how a lymphopaenic environment enables tumour-reactive T cells to destroy large burdens of metastatic tumour and how the state of differentiation of the adoptively transferred T cells can affect the outcome of treatment. We also discuss how the translation of these new findings might further improve the efficacy of adoptive cell transfer through the u...

  9. Characterization of Early and Late Adopters of ATM Card in Indian Banking Industry

    Directory of Open Access Journals (Sweden)

    Kamalpreet Kaur

    2012-10-01

    Full Text Available The present study deals with affect of adoption pattern of the ATMs by banks on its characteristics. With the exploration of various characteristics of the banks like Size, Profi tability, Efficiency, Cost of Operations, Asset quality and Credit risk, Financing Pattern, Diversifi cation and Age etc.; the study has tried to differentiate between the early and late adopter category of the banks regarding ATM cards. The banks have been categorized into early and late adopters on the basis of their adoption period. For this purpose, 50 scheduled commercial banks consisting of 27 Public Sector Banks and 23 Private Sector Banks have been taken as sample to investigate the various aspects of and early adopter banks in comparison to late adopter banks. The time period of the study is 20 years i.e. from 1991 to 2010. It can be concluded that the initiators and fi rst movers take advantage over the late adopters and laggards. They have found to perform better in terms of various parameters. Overall, the early adopter banks are larger in size, more diversifi ed, having lesser branches, more market share and wide ATM network as compared to late adopter ones. Thus, the empirical results evidently reveal that the both the groups have their own different characteristics.

  10. Relación entre el Parque de Tractores Agrícolas y el Patrón de Difusión y Adopción mediante un Modelo Logístico Relationship between the Agricultural Tractor Stock and the Adoption and Diffusion Patterns using a Logistic Model

    Directory of Open Access Journals (Sweden)

    Ricardo M Muñoz

    2011-01-01

    Full Text Available Este estudio determina el parque de tractores y la evolución del crecimiento de la adopción agregada del tractor agrícola en Chile. Se aplicó un modelo logístico para determinar el crecimiento de la adopción y la rapidez de adopción del tractor agrícola. Los parámetros del modelo se determinaron mediante un procedimiento no lineal. Se usó el balance del parque de tractores y la curva de tractorización y de adopción para estimar la variación de las unidades entre t y t-1 y se relacionó con la incorporación de tractores y con aquellos dados de baja. El modelo logístico mostró un nivel de ajuste significativo y permitió estimar la tasa de adopción y el nivel de saturación de la adopción. Se concluye que la metodología se puede aplicar para el estudio de la adopción de tecnologías en otras regiones y otros países.This paper determines the tractor stock and aggregate adoption growth of agricultural tractors in Chile. A logistic model was applied to determine the adoption growth and the adoption speed of agricultural tractors. Model parameters were determined by a non-linear algorithm. The tractor stock balance and the tractorization curve were used to estimate variation rate between t and t-1. This variation was associated with the introduction of tractors and those units removed from the stock. The logistic model showed a significant adjustment level and allowed estimating the rate of adoption and the adoption saturation level. It is concluded that the methodology can be applied to study technological adoption in other regions and other countries.

  11. Methodological findings in studies on adoptive families

    Directory of Open Access Journals (Sweden)

    Kalus, Alicja

    2014-09-01

    Full Text Available The issue of adoptive families is the object of interest for many researchers, especially outside Polish borders. A critical analysis of previous studies on adoption shows that the scientific value of many of the existing studies raises doubts. The reasons of this are more or less significant methodological weaknesses, which significantly reduce their scientific value. Researchers often focus on the study of individuals in the adoptive family, e.g. mothers only, adopted children only or selected types of relationships within the family e.g. the mother – child relation. There is a little amount of studies on family systems. It seems worthy to note that numerous studies are conducted in the cause-effect model of explaining phenomena in the adoptive family. Such approach to the study of reality, which is the family, is regarded as too big simplification. Therefore, there is an important reason to undertake the scientific discourse on the research methodology on the adoptive family. Discussed in the paper issues include: theoretical models, arrangement of studies, especially the selection of the test group and the research methods used in the study on adoptive families. Conducting scientific research is essential for the adoption practice. Among psychologists-practitioners exists a disturbing belief that working with adoptive families can be based on the experience gained in psychological practice. This fact can be explained by the existing lack of willingness of adoptive families to cooperate in the field of scientific research. Lack of this cooperation significantly limits the access to the study population of adoptive families.

  12. Predictors of race, adoption, and sexual orientation related socialization of adoptive parents of young children.

    Science.gov (United States)

    Goldberg, Abbie E; Smith, JuliAnna Z

    2016-04-01

    Using a sample of 125 lesbian, gay, and heterosexual adoptive parent couples with young children (M = 6.32 years), this study examined predictors of direct socialization (preparation for adoptism, racism, and heterosexism) and indirect socialization (modeling interactions by responding to outsiders' inquiries about their child's adoptive status, racial background, or family structure). In terms of direct socialization, parents of older children tended to engage in more socialization around adoptism and heterosexism, and parents of daughters tended to engage in more socialization around racism and heterosexism. Greater perceived child interest in adoption was related to more direct socialization around adoptism. Parents of color reported more direct socialization around racism. Having a child of color was related to more direct socialization around heterosexism. Regarding indirect socialization, sexual minority parents reported more socialization around adoption and race. Greater perceived child interest in adoption was related to more indirect adoption socialization. Being more "out" was related to more indirect socialization around parent sexual orientation.

  13. Predictors of race, adoption, and sexual orientation related socialization of adoptive parents of young children.

    Science.gov (United States)

    Goldberg, Abbie E; Smith, JuliAnna Z

    2016-04-01

    Using a sample of 125 lesbian, gay, and heterosexual adoptive parent couples with young children (M = 6.32 years), this study examined predictors of direct socialization (preparation for adoptism, racism, and heterosexism) and indirect socialization (modeling interactions by responding to outsiders' inquiries about their child's adoptive status, racial background, or family structure). In terms of direct socialization, parents of older children tended to engage in more socialization around adoptism and heterosexism, and parents of daughters tended to engage in more socialization around racism and heterosexism. Greater perceived child interest in adoption was related to more direct socialization around adoptism. Parents of color reported more direct socialization around racism. Having a child of color was related to more direct socialization around heterosexism. Regarding indirect socialization, sexual minority parents reported more socialization around adoption and race. Greater perceived child interest in adoption was related to more indirect adoption socialization. Being more "out" was related to more indirect socialization around parent sexual orientation. PMID:26371450

  14. Adopters and non-adopters of wood pellet heating in Norwegian households

    International Nuclear Information System (INIS)

    The aim of the present paper is to understand the differences between adopters and non-adopters of wood pellet heating in Norwegian households by comparing the two groups with regard to key points of adoption. A mail survey of 669 adopters and 291 non-adopters of wood pellet heating was conducted in 2008. Results indicate that there are significant differences between groups with respect to socio-demographic factors, decision-related factors, heating systems adopted and reasons for shifting heating systems. The results also indicate that the adopter group shows characteristics of early adopters, whereas the non-adopter group has characteristics of late adopters. The results for levels of income and education contradict what would be predicted from theory, however. Both groups show no significant difference with respect to values, but the perception of which heating system is the most environmentally friendly differs significantly. The top three reasons cited by the adopter group for installing wood pellet heating are getting an environmentally friendly heating system, low operation costs and an anticipated increase in electricity prices. According to the non-adopter group, the main barriers to adoption are high installation costs, followed by the difficulties of refitting the house for wood pellet heating. A higher subsidy (i.e., an average of 64% of the total installation cost) rather than the current subsidy of up to 20% was required by the non-adopter group to switch to wood pellet heating. (author)

  15. Adopters and non-adopters of wood pellet heating in Norwegian households

    Energy Technology Data Exchange (ETDEWEB)

    Sopha, Bertha Maya; Hertwich, Edgar G. [Industrial Ecology Programme and Department of Energy and Process Engineering, Norwegian University of Science and Technology, NO-7491 Trondheim (Norway); Kloeckner, Christian A. [Department of Psychology, Section for Risk Psychology, Environment and Safety (RIPENSA), Norwegian University of Science and Technology, NO-7491 Trondheim (Norway)

    2011-01-15

    The aim of the present paper is to understand the differences between adopters and non-adopters of wood pellet heating in Norwegian households by comparing the two groups with regard to key points of adoption. A mail survey of 669 adopters and 291 non-adopters of wood pellet heating was conducted in 2008. Results indicate that there are significant differences between groups with respect to socio-demographic factors, decision-related factors, heating systems adopted and reasons for shifting heating systems. The results also indicate that the adopter group shows characteristics of early adopters, whereas the non-adopter group has characteristics of late adopters. The results for levels of income and education contradict what would be predicted from theory, however. Both groups show no significant difference with respect to values, but the perception of which heating system is the most environmentally friendly differs significantly. The top three reasons cited by the adopter group for installing wood pellet heating are getting an environmentally friendly heating system, low operation costs and an anticipated increase in electricity prices. According to the non-adopter group, the main barriers to adoption are high installation costs, followed by the difficulties of refitting the house for wood pellet heating. A higher subsidy (i.e., an average of 64% of the total installation cost) rather than the current subsidy of up to 20% was required by the non-adopter group to switch to wood pellet heating. (author)

  16. Covering Adoption: General Depictions in Broadcast News

    Science.gov (United States)

    Kline, Susan L.; Karel, Amanda I.; Chatterjee, Karishma

    2006-01-01

    Using theories of stigma (Goffman, 1963) and media frames (Iyengar, 1991), 292 news stories pertaining to adoption that appeared on major broadcast networks between 2001 and 2004 were analyzed. Media coverage of adoptees contained more problematic than positive depictions. Although birth parents were not always depicted, adoptive parent and…

  17. Intercountry Adoption Agencies and the HCIA

    NARCIS (Netherlands)

    P. Selman (Peter)

    2014-01-01

    textabstractThis report discusses concerns raised by participants of Thematic Area 3 (Intercountry Adoption Agencies and the HCIA) of the International Forum on Intercountry Adoption and Global Surrogacy held in August 2014. The aim is to report the views of those participating in this area on the i

  18. Predicting Language Outcomes for Internationally Adopted Children

    Science.gov (United States)

    Glennen, Sharon L.

    2007-01-01

    Purpose: Language and speech are difficult to assess in newly arrived internationally adopted children. The purpose of this study was to determine if assessments completed when toddlers were first adopted could predict language outcomes at age 2. Local norms were used to develop early intervention guidelines that were evaluated against age 2…

  19. 77 FR 66517 - National Adoption Month, 2012

    Science.gov (United States)

    2012-11-06

    ... Sig.) [FR Doc. 2012-27190 Filed 11-5-12; 8:45 am] Billing code 3295-F3 ... Documents#0;#0; ] Proclamation 8896 of November 1, 2012 National Adoption Month, 2012 By the President of... knowing the love and protection of a permanent family. During National Adoption Month, we give voice...

  20. 78 FR 66609 - National Adoption Month, 2013

    Science.gov (United States)

    2013-11-05

    .... (Presidential Sig.) [FR Doc. 2013-26669 Filed 11-4-13; 11:15 am] Billing code 3295-F4 ... Documents#0;#0; ] Proclamation 9049 of October 31, 2013 National Adoption Month, 2013 By the President of... million children and teenagers. During National Adoption Month, we celebrate these families and...

  1. 76 FR 68613 - National Adoption Month, 2011

    Science.gov (United States)

    2011-11-04

    ... Sig.) [FR Doc. 2011-28839 Filed 11-3-11; 11:15 am] Billing code 3295-F2-P ... Documents#0;#0; ] Proclamation 8744 of November 1, 2011 National Adoption Month, 2011 By the President of... basic support, and still more children abroad live without families. During National Adoption Month,...

  2. Internationally Adopted Children: Important Information for Parents

    Science.gov (United States)

    ... Parents Page Content Article Body Congratulations on the adoption of your child! As you begin your new life together, it ... with you, particularly ones you received before the child’s adoption.) Review risk factors from your child’s history . This ...

  3. 32 CFR 584.4 - Adoption proceedings.

    Science.gov (United States)

    2010-07-01

    ... situations were a soldier is trying to adopt a child. It applies to those situations where another person is trying to adopt a legitimate or illegitimate child of a soldier. A child born in or out of wedlock... has stated that he or she is not the natural parent of the child. (v) Since the soldier is not...

  4. Adoption of communication technologies and national culture

    NARCIS (Netherlands)

    Hofstede, G.J.

    2001-01-01

    This paper investigates the question what attributes of countries influence the differential speed at which they adopt new communication technologies. On the basis of empirical data, it concludes that besides GNP per capita, cultural variables predict the speed of technology adoption. In particular,

  5. Fuzzy Cognitive Map Modelling Educational Software Adoption

    Science.gov (United States)

    Hossain, Sarmin; Brooks, Laurence

    2008-01-01

    Educational software adoption across UK secondary schools is seen as unsatisfactory. Based on stakeholders' perceptions, this paper uses fuzzy cognitive maps (FCMs) to model this adoption context. It discusses the development of the FCM model, using a mixed-methods approach and drawing on participants from three UK secondary schools. The study…

  6. Are You Pregnant and Thinking about Adoption?

    Science.gov (United States)

    ... Understanding Adoption Adoption is a process—with legal, social, and emotional aspects—in which children who will not be raised ... can communicate in various ways—letters, phone calls, ... The type and frequency of communication will depend on the choices and needs of ...

  7. Faculty Adoption of Active Learning Classrooms

    Science.gov (United States)

    Van Horne, Sam; Murniati, Cecilia Titiek

    2016-01-01

    Although post-secondary educational institutions are incorporating more active learning classrooms (ALCs) that support collaborative learning, researchers have less often examined the cultural obstacles to adoption of those environments. In this qualitative research study, we adopted the conceptual framework of activity theory to examine the…

  8. ICT as an Enabler for Innovation Adoption

    NARCIS (Netherlands)

    M. Verkerk (Michel); G.J. van der Pijl (Gert); E. van Asperen (Eelco)

    2009-01-01

    textabstractIn this paper we investigate how two online services (a portal and a digital library) may influence the adoption of an innovation. It is known from prior surveys that the installation services branch of the Dutch building industry has a relatively slow adoption rate for innovations. We e

  9. Adopting the Caregiver Role: A Family Legacy.

    Science.gov (United States)

    Piercy, Kathleen W.; Chapman, Jeffery G.

    2001-01-01

    A qualitative study was done to investigate how adult children become caregivers to older parents with functional impairments and what roles their own children adopt in their family's care arrangement. Interviews revealed five influences on children and grandchildren's adoption of caregiver roles: expectations; family rules; religious training;…

  10. The geography of Internet adoption by retailers

    NARCIS (Netherlands)

    Weltevreden, J.W.J.; Atzema, O.A.L.C.; Frenken, K.; Kruif, K. de; Oort, F.G. van

    2005-01-01

    Up till now, the literature on Internet adoption by retailers paid little attention to spatial variables. Using data on 27,000 retail outlets in the Netherlands, we investigate the geographical diffusion of Internet adoption by Dutch retailers. More precise, we examine to what extent retail Internet

  11. Euro adoption in Romania: Facts and perceptions

    OpenAIRE

    I.-C. Enache; Z. Brodský

    2013-01-01

    The aim of this paper is to link the nominal convergence criteria with Romanian citizens perceptions about euro adoption. Based on convergence reports and Euro-barometer data, the 2010 and 2012 years are analysed. It is shown that even if the nominal convergence achievements are improving, the perceptions regarding the euro adoption are deteriorating. A better communication strategy is advised.

  12. Understanding producers' motives for adopting sustainable practices

    NARCIS (Netherlands)

    Trujillo-Barrera, Andres; Pennings, Joost M.E.; Hofenk, Dianne

    2016-01-01

    Understanding the motives and risk attitudes of producers to engage in sustainable practices is important for policy-makers who wish to increase the likelihood of adoption and improve the design of incentives. This article examines the underlying motives of producers to adopt sustainable practice

  13. Studies based on the Danish Adoption Register

    DEFF Research Database (Denmark)

    Petersen, Liselotte; Sørensen, Thorkild I A

    2011-01-01

    INTRODUCTION: The genetic and familial environmental influences making up familial correlations in traits and familial aggregation of diseases may be disentangled by adoption studies. Thus, resemblance between the adoptee and the biological relatives indicates a contribution of the segregating gene...... they have in common, and resemblance between the adoptee and the rearing adoptive family members indicates influences of the shared family environment. The Danish Adoption Register includes information on all 14,425 non-familial adoptions of Danish children legally granted in Denmark from 1924 to 1947....... RESEARCH TOPICS: To illustrate the adoption study approach in research, we present results achieved in schizophrenia, body fatness, smoking, and mortality. Significantly higher prevalence of schizophrenia and related conditions were found in biological relatives to adoptees with schizophrenia than among...

  14. Holistic BIM Adoption and Diffusion in Malaysia

    Directory of Open Access Journals (Sweden)

    Mamter Shahela

    2016-01-01

    Full Text Available Building Information Modeling is modeling technology and associated set of processes to produce, communicate and analyze digital information models for construction life cycle. Although many benefits can gain by the implementation of BIM, the pace adoption of BIM in Malaysia is still slow. There is an abundance of theories and models purporting to describe the drivers and mechanisms of how new technologies are adopted or diffused into a particular section of society or by individuals or stake players. In addition, many acceptance theories, which imply the adoption of new technologies and can be applied in every sector. This paper aims to present a review of the existing literature of BIM adoption and diffusion theory/model/framework in Malaysia. Researcher revealed the list of driven factors of Holistic BIM Adoption. In addition, it will deal with how to work effectively with groups that initially are relatively

  15. Non-Adoption of Best Management Practices: Demographics and Adoption Constraints of Oklahoma Cattle Producers

    OpenAIRE

    Schumacher, Stephanie; Peel, Derrell S.; Raper, Kellie Curry

    2013-01-01

    Using survey data from Oklahoma Cow-Calf producers, a binomial logit model is employed to estimate the probability of non-adoption of specific management or marketing practices based on producers' demographics. Thirteen individual management and marketing practices are examined. Additional logit models examine relationships between non-adopters' characteristics and reasons given for non-adoption.

  16. Banking on the equator. Are banks that adopted the equator principles different from non-adopters?

    NARCIS (Netherlands)

    Scholtens, B.; Dam, L.

    2007-01-01

    We analyze the performance of banks that adopted the Equator Principles. The Equator Principles are designed to assure sustainable development in project finance. The social, ethical, and environmental policies of the adopters differ significantly from those of banks that did not adopt the Equator P

  17. Parallel Process Issues for Lesbian and Gay Adoptive Parents and Their Adopted Children

    Science.gov (United States)

    Matthews, John D.; Cramer, Elizabeth P.

    2005-01-01

    Gays and lesbians, both single and coupled, are increasingly turning to adoption to create or expand their families. This manuscript specifically addresses the continuing needs of adoptees and adoptive parents by exploring key issues in the life course of gays and lesbians and their adopted children, and identifying potential parallel development…

  18. Cultural Tourism in Transnational Adoption: "Staged Authenticity" and Its Implications for Adopted Children

    Science.gov (United States)

    Quiroz, Pamela Anne

    2012-01-01

    The discursive practices of adoptive parents in two online transnational adoption forums (2006-2008) and observations of five international adoption workshops suggest that what Heather Jacobson described as "culture keeping", the cultural socialization of children that retains a sense of native group identity, is more aptly characterized as…

  19. Taxation and technology adoption: a hotelling approach

    OpenAIRE

    Kriechel, B.; Ziesemer, T.H.W.

    2006-01-01

    Environmental regulation and competitiveness are issues that seem to be at odds. However, the `Porter Hypothesis' states that firms can actually gain in competitiveness if they are subject to stricter environmental regulation. We show in a simple model the basic setting of the problem to apply it then to a Hotelling framework. A non-adoption tax (adoption subsidy) is shown to destroy a non-adoption equilibrium in a closed economy model. We show that taxes not directly targeting the non-adopti...

  20. ICT Adoption in Facilities Management Supply Chain

    DEFF Research Database (Denmark)

    Scupola, Ada

    2012-01-01

    This article involves a qualitative study of factors impacting the adoption of ICT solutions in the Danish facility management supply chain. The results show that there are a number of drivers and barriers that influence the adoption of ICT solutions in this service sector. These have been grouped...... under three major categories: organizational factors including strategic and operational factors; external environmental factors including supplier interdependence and industry characteristics; and technological factors including compatibility and complexity. The study is relevant to researchers...... concerned with ICT adoption, operations and service management (especially facilities management) as well as operation managers and ICT managers....

  1. Adoptive immunotherapy of cancer using CD4+ T cells

    OpenAIRE

    Muranski, Pawel; Restifo, Nicholas P

    2009-01-01

    CD4+ T cells are central to the function of the immune system but their role in tumor immunity remains underappreciated. It is becoming clear that there is an enormous diversity of CD4+ T cell polarization patterns including Th1, Th2, Th17, and regulatory T cells (Tregs). These functionally divergent T cell subsets can have opposing effects — they can trigger tumor rejection or inhibit treatment after adoptive cell transfer. Some polarized CD4+ cells have plasticity, and their phenotypes and ...

  2. Methodology for Web Services Adoption Based on Technology Adoption Theory and Business Process Analyses

    Institute of Scientific and Technical Information of China (English)

    AN Liping; YAN Jianyuan; TONG Lingyun

    2008-01-01

    Web services use an emerging service-oriented architecture for distributed computing. Many organizations are either in the process of adopting web services technology or evaluating this option for incorporation into their enterprise information architectures. Implementation of this new technology requires careful assessment of the needs and capabilities of an organization to formulate adoption strategies. This paper presents a methodology for web services adoption based on technology adoption theory and business process analyses. The methodology suggests that strategies, business areas, and functions within an organization should be considered based on the existing organizational information technology status during the process of adopting web services to support the business needs and requirements.

  3. Improvisation during Process-Technology Adoption

    DEFF Research Database (Denmark)

    Tjørnehøj, Gitte; Mathiassen, Lars

    2010-01-01

    Most software firms struggle to take advantage of the potential benefits of software process improvement (SPI) as they adopt this technology into the complex and dynamic realities of their day-to-day operation. Such efforts are therefore typically fluctuating between management's attempt to control...... SPI technology adoption and events that causes the process to drift in unpredictable directions. To further understand how management's attempt to control the process is complemented by drifting, this article investigates the role of improvisation in adoption of SPI technology in a Danish software...... firm, SmallSoft, over a 10-year period (1996–2005). We found that micro-level and macro-level improvisations interacted, often in uncoordinated ways, to shape SPI technology adoption at SmallSoft. The improvisations enhanced employee creativity, motivation and empowerment, created momentum...

  4. Improvisation during Process-Technology Adoption

    DEFF Research Database (Denmark)

    Tjørnehøj, Gitte; Mathiassen, Lars

    2010-01-01

    Most software firms struggle to take advantage of the potential benefits of software process improvement (SPI) as they adopt this technology into the complex and dynamic realities of their day-to-day operation. Such efforts are therefore typically fluctuating between management's attempt to control...... SPI technology adoption and events that causes the process to drift in unpredictable directions. To further understand how management's attempt to control the process is complemented by drifting, this article investigates the role of improvisation in adoption of SPI technology in a Danish software...... firm, SmallSoft, over a 10-year period (1996-2005). We found that micro-level and macro-level improvisations interacted, often in uncoordinated ways, to shape SPI technology adoption at SmallSoft. The improvisations enhanced employee creativity, motivation and empowerment, created momentum...

  5. Role of value compatibility in IT adoption

    DEFF Research Database (Denmark)

    Bunker, Deborah; Kautz, Karlheinz; Nguyen, Anne Luu Thanh

    2007-01-01

    Compatibility has been recognised as an important element in the adoption of IT innovations in organisations but as a concept it has been generally limited to technical or functional factors. Compatibility is also significant, however, with regard to value compatibility between the organisation......, and the adopted IT innovation. We propose a framework to determine value compatibility analysing the organisation's and information system's structure, practices and culture, and explore the value compatibility of an organisation with its adopted self-service computer-based information system. A case study......-service acceptance and training issues experienced by the case organisation. These findings add insight into the problems experienced with value compatibility and the adoption of the information systems, and show the potential use of the proposed framework in the detection of such problems.Journal of Information...

  6. Adopting Integrated Pest Management in Schools.

    Science.gov (United States)

    Currie, William E.

    1991-01-01

    The development of an effective Integrated Pest Management program is discussed. Provided are the common goals and procedures involved in adopting an Integrated Pest Management program for schools. (CW)

  7. Distributed PV Adoption in Maine Through 2021

    Energy Technology Data Exchange (ETDEWEB)

    Gagnon, Pieter; Sigrin, Ben

    2015-11-06

    NREL has used its dSolar (distributed solar) model to generate low-medium-high estimates of distributed PV adoption in Maine through 2021. This presentation gives a high-level overview of the model and modeling results.

  8. Adopt a Pixel Photographs: 2013-Present

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The photographs in the Adopt a Pixel collection were provided by volunteers with a digital camera, a Global Positioning System (GPS), and a compass or a smart...

  9. Organizational Green IT Adoption: Concept and Evidence

    Directory of Open Access Journals (Sweden)

    Qi Deng

    2015-12-01

    Full Text Available Green IT has emerged as an important research topic in information systems and in other areas, such as business sustainability management. Some progress has been made in our understandings of green IT in a wide area of research topics, ranging from the green IT definition to the motivation for adopting green IT by organizations. This paper provides a holistic review and explanation of why organizations adopt green IT. Based on an extensive review of extant studies and a broad theoretical foundation, the paper presents a theoretical framework on organizational green IT adoption (OGITA. For researchers, the study provides a comprehensive review of previous green IT adoption studies and a roadmap for future research. For practitioners, the study provides managers and policy makers a systematic analytical framework in guiding their business decisions.

  10. International Adoption: The Human Rights Position

    OpenAIRE

    Bartholet, Elizabeth

    2009-01-01

    International adoption is under siege, with the number of children placed dropping each of the last several years, and many countries imposing severe new restrictions. Key forces mounting the attack claim the child human rights mantle, arguing that such adoption denies heritage rights, and often involves abusive practices. Many nations assert rights to hold onto the children born within their borders, and others support these demands citing subsidiarity principles. But children’s most basic h...

  11. The Adoption and Diffusion of Service Products

    OpenAIRE

    Myung Joong Kwon

    2003-01-01

    The objective of this paper is to theoretically explore the innovation, adoption and diffusion of service product. A theoretical model of the diffusion of service product is developed that takes account of transportation, waiting and searching casts in the adoption of service product. The main results of the model are; (1) the diffusion of service product is slower than that of the manufacturing equivalent and (2) the delivery or retail distribution service speeds up the diffusion of the manu...

  12. Managing risk in open source software adoption

    OpenAIRE

    Franch Gutiérrez, Javier; Susi, Angelo; Annosi, Maria Carmela; Ayala Martínez, Claudia Patricia; Glott, Ruediger; Gross, Daniel; Kenett, Ron; Mancinelli, Fabio; Ramsany, Pop; Thomas, Cedric; Ameller, David; Bannier, Stijn; Bergida, Nili; Blumenfeld, Yehuda; Bouzereau, Olivier

    2013-01-01

    By 2016 an estimated 95% of all commercial software packages will include Open Source Software (OSS). This extended adoption is yet not avoiding failure rates in OSS projects to be as high as 50%. Inadequate risk management has been identified among the top mistakes to avoid when implementing OSS-based solutions. Understanding, managing and mitigating OSS adoption risks is therefore crucial to avoid potentially significant adverse impact on the business. In this position paper we portray a sh...

  13. Adoption of interorganisational ICT in primary care.

    OpenAIRE

    Plomp, M.G.A.; Batenburg, R.S.; Verheij, R.A.

    2011-01-01

    Efficient and effective collaboration among health care providers is of great importance. Interorganisational ICT can enable and facilitate this collaboration, but the adoption of such information systems is still sparsely analysed. In this paper we describe the results of a survey among 49 GP practices in The Netherlands held in 2009 and 2010, which were queried on their adoption of different types of interorganisational ICT, such as the exchange with out-of-hours services and with other pri...

  14. Internet Banking Adoption Factors in Finland

    OpenAIRE

    Minna Mattila; Heikki Karjaluoto; Tapio Pento,

    2001-01-01

    This paper finds out factors which have defined consumers' adoption of Internet banking in Finland. Finland is a world leader in electronic banking, and over 39.8 percent of all the banking transactions were made over the Internet. Using the data of a large survey, we develop a cognitive model of the factors which affect the adoption of Internet banking. Prior technology experience, personal banking experience, reference group influence, and security concerns are found to be the main factors,...

  15. Determinants of Internet Banking Adoption in Jordan

    OpenAIRE

    Haneen A. Abu-Assi; Hani H. Al-Dmour; Zu'bi M.F. Al-Zu'bi

    2014-01-01

    The primary purpose of internet banking research is to investigate the factors that influence the E-Banking adoption of clients who have internet access. To develop a theoretical framework model in the context of this study, the literature that relates to the Technology Acceptance Model (TAM), Diffusion of Innovations (DOI), Technology Readiness Index (TRI) and their various adaptations were undertaken. Internet banking has been considered an innovative service, whose potential adoption by cl...

  16. Beyond preadoptive risk: The impact of adoptive family environment on adopted youth's psychosocial adjustment.

    Science.gov (United States)

    Ji, Juye; Brooks, Devon; Barth, Richard P; Kim, Hansung

    2010-07-01

    Adopted children often are exposed to preadoptive stressors--such as prenatal substance exposure, child maltreatment, and out-of-home placements--that increase their risks for psychosocial maladjustment. Psychosocial adjustment of adopted children emerges as the product of pre- and postadoptive factors. This study builds on previous research, which fails to simultaneously assess the influences of pre- and postadoptive factors, by examining the impact of adoptive family sense of coherence on adoptee's psychosocial adjustment beyond the effects of preadoptive risks. Using a sample of adoptive families (n = 385) taking part in the California Long Range Adoption Study, structural equation modeling analyses were performed. Results indicate a significant impact of family sense of coherence on adoptees' psychosocial adjustment and a considerably less significant role of preadoptive risks. The findings suggest the importance of assessing adoptive family's ability to respond to stress and of helping families to build and maintain their capacity to cope with stress despite the sometimes fractious pressures of adoption.

  17. Consumer Adoption Challenges To The Smart Grid

    Directory of Open Access Journals (Sweden)

    Anu Gupta

    2012-12-01

    Full Text Available The smart grid represents the next significant evolution of the power infrastructure with many enhancements and challenges. This paper provides a qualitative review of the key consumer adoption challenges of the smart grid. This literature review takes disparate pieces of work and identifies strategic research focal areas. Through existing research and media documents, it identifies the primary consumer adoption challenges as privacy, Radio Frequency (RF safety, and power rate increases. It also provides a review of each adoption challenge. The review is applied to the Canadian perspective drawing from smart grid experiences across North America. The review demonstrates that each of the challenge areas has a negative impact on the adoption and support of the smart grid. This paper recommends further in-depth research be conducted in the following areas: testing each of the consumer adoption challenges—privacy, RF Safety, and rate increases—separately with quantitative measures; testing the willingness to accept a power infrastructure with the security and stability level of a bank; and testing the impact that proactively educating the public would have on the smart grid adoption.

  18. ADOPTION OF BIOSECURITY MEASURES BY LAYER SMALLHOLDERS

    Directory of Open Access Journals (Sweden)

    V. S. Lestari

    2011-12-01

    Full Text Available It was indicated that layer smallholders awareness of biosecurity was low. This paper aimed to determine the level of adoption within the South Sulawesi layer smallholders of a range of standard biosecurity measures. Sidenreng Rappang (Sidrap regency was chosen as a location of the research, because it was famous as a central of layer smallholders. Total sample was 60 respondents. The sample was chosen through random sampling from two districts which were the most populous of layer smallholders, namely Baranti and Maritengae. Data were collected using structured questionnaires and depth-interview. The data were tabulated and analysed using a simple method of scoring with regard to their biosecurity status. The status of biosecurity was used to know the level of biosecurity adoption. Biosecurity status was obtained based on the adoption of biosecurity measures which consisted of 9 stages: farm inputs, traffic onto farms, distance from sources of pathogens to shed, exposure of farm, biosecurity at farm boundary, biosecurity between farm boundary and shed, biosecurity at the shed door, traffic into the shed and susceptibility of the flock. Using adoption index, this research revealed that biosecurity adoption of layer smallholders in South Sulawesi was classified into a partial adopter.

  19. 5 CFR 843.404 - Proof of adoption.

    Science.gov (United States)

    2010-01-01

    ... 5 Administrative Personnel 2 2010-01-01 2010-01-01 false Proof of adoption. 843.404 Section 843... Proof of adoption. (a) An adopted child is— (1) A child adopted by the employee or retiree before the... petition for adoption was filed by the employee or retiree and who is adopted by the current spouse of...

  20. The Practice of Adoption: History, Trends, and Social Context

    Science.gov (United States)

    Zamostny, Kathy P.; O'Brien, Karen M.; Baden, Amanda L.; Wiley, Mary O'Leary

    2003-01-01

    This article presents an overview of the practice of adoption to counseling psychologists to promote clinical understanding of the adoption experience and to stimulate research on adoption. The article includes definitions of adoption terminology, important historical and legal developments for adoption, a summary of adoption statistics,…

  1. Adoption Activities on the Internet: A Call for Regulation

    Science.gov (United States)

    Roby, Jini L.; White, Holly

    2010-01-01

    There is a growing practice of adoption services on the Internet with varying degrees of regulation, depending on whether it is domestic infant adoption, public foster care adoption, or international adoption. Regulation is particularly lacking in domestic infant adoptions, with Web sites connecting prospective birth and adoptive parents,…

  2. Moses and Superman Come Home: Counseling Adoptees and Adoptive Families.

    Science.gov (United States)

    Saiz, Stephen G.

    This paper looks at three parties impacted by adoption: the adoptive parents, the adopted child, and the adoptive family. When working with adoptive parents, counselors should respect the strength of the couple, their commitment to parenthood, and the closeness that may develop from weathering the issue of childlessness. Adoptive parents are…

  3. Assessing access for prospective adoptive parents living with HIV: an environmental scan of Ontario's adoption agencies.

    Science.gov (United States)

    Underhill, Angela A; Kennedy, V Logan; Lewis, Johanna; Ross, Lori E; Loutfy, Mona

    2016-10-01

    Work has been underway to increase the availability of parenting options for people living with and affected by HIV. One option, adoption, has not yet been explored in the literature. The study aimed to gain a better understanding of the potential of adoption for individuals/couples living with HIV in Ontario, and to assess potential structural barriers or facilitators that may impact their experience navigating the adoption system by conducting an environmental scan of adoption service providers in Ontario. A list of adoption service providers was compiled using the Ontario government's website. Information relevant to the study's measures was collected using service providers' websites. Service providers without websites, or with websites that did not address all of the research measures, were contacted via telephone to complete a structured interview. Online data extraction was possible for 2 and telephone surveys were completed with 75 adoption service providers (total n = 77). Most service providers reported that HIV status is not an exclusion criterion for prospective parents (64%). However, more than one-fifth of the participants acknowledged they were not sure if people with HIV were eligible to adopt. Domestic service providers were the only providers who did not report knowledge of restrictions due to HIV status. Private domestic adoption presented social barriers as birth parent(s) of a child can access health records of a prospective parent and base their selection of an adoptive parent based on health status. Adoption practitioners and licensees involved in international adoptions reported the most structural barriers for prospective parent(s) living with HIV, attributed to the regulations established by the host country of the child(ren) eligible for adoption. Although international adoptions may present insurmountable barriers for individuals living with HIV, public and private domestic adoption appears to be a viable option. PMID:27136971

  4. Executive function and mental health in adopted children with a history of recreational drug exposures.

    Directory of Open Access Journals (Sweden)

    Brian J Piper

    Full Text Available Adoptive children are at increased risk for problematic behaviors but the origin of these individual differences in neurobehavioral function is unclear. This investigation examined whether adopted children with prenatal exposure to a wide variety of recreational drugs exhibited higher scores (i.e. more problems with executive function and psychiatric symptomology. Caregivers of children ages 5 to 18 completed an online survey with items about use of alcohol, nicotine, or methamphetamine during pregnancy followed by the Behavior Rating Inventory of Executive Function (BRIEF, N = 437 including 59 adoptive parents or the Child Behavior Checklist (CBCL, N = 549 including 54 adoptive parents. Relative to a comparison group of children raised by their biological parents, adoptive children that were polysubstance exposed during prenatal development exhibited higher rates of academic difficulties and were behind their classmates in math and reading. Adoptive children had statistically and clinically significant higher BRIEF ratings and this pattern was similar for boys and girls. CBCL ratings were significantly increased in adoptive children, particularly for Externalizing and Attention problems. Adoptive children with a history of polysubstance exposures including alcohol, nicotine, and methamphetamine are at heightened risk for difficulties with executive function as well as various psychopathologies. These findings suggest that increased monitoring to identify and implement remediation strategies may be warranted for adopted children with a history of in utero drug exposures.

  5. Executive function and mental health in adopted children with a history of recreational drug exposures.

    Science.gov (United States)

    Piper, Brian J; Gray, Hilary M; Corbett, Selena M; Birkett, Melissa A; Raber, Jacob

    2014-01-01

    Adoptive children are at increased risk for problematic behaviors but the origin of these individual differences in neurobehavioral function is unclear. This investigation examined whether adopted children with prenatal exposure to a wide variety of recreational drugs exhibited higher scores (i.e. more problems) with executive function and psychiatric symptomology. Caregivers of children ages 5 to 18 completed an online survey with items about use of alcohol, nicotine, or methamphetamine during pregnancy followed by the Behavior Rating Inventory of Executive Function (BRIEF, N = 437 including 59 adoptive parents) or the Child Behavior Checklist (CBCL, N = 549 including 54 adoptive parents). Relative to a comparison group of children raised by their biological parents, adoptive children that were polysubstance exposed during prenatal development exhibited higher rates of academic difficulties and were behind their classmates in math and reading. Adoptive children had statistically and clinically significant higher BRIEF ratings and this pattern was similar for boys and girls. CBCL ratings were significantly increased in adoptive children, particularly for Externalizing and Attention problems. Adoptive children with a history of polysubstance exposures including alcohol, nicotine, and methamphetamine are at heightened risk for difficulties with executive function as well as various psychopathologies. These findings suggest that increased monitoring to identify and implement remediation strategies may be warranted for adopted children with a history of in utero drug exposures. PMID:25337917

  6. Parent adjustment over time in gay, lesbian, and heterosexual parent families adopting from foster care.

    Science.gov (United States)

    Lavner, Justin A; Waterman, Jill; Peplau, Letitia Anne

    2014-01-01

    Although increasing numbers of gay and lesbian individuals and couples are adopting children, gay men and lesbian women continue to face increased scrutiny and legal obstacles from the child welfare system. To date, little research has compared the experiences of gay or lesbian and heterosexual adoptive parents over time, limiting conceptual understandings of the similarities they share and the unique challenges that gay and lesbian adoptive parents may face. This study compared the adoption satisfaction, depressive symptoms, parenting stress, and social support at 2, 12, and 24 months postplacement of 82 parents (60 heterosexual, 15 gay, 7 lesbian) adopting children from foster care in Los Angeles County. Few differences were found between heterosexual and gay or lesbian parents at any of the assessments or in their patterns of change over time. On average, parents in both household types reported significant increases in adoption satisfaction and maintained low, nonclinical levels of depressive symptoms and parenting stress over time. Across all family types, greater parenting stress was associated with more depressive symptoms and lower adoption satisfaction. Results indicated many similarities between gay or lesbian and heterosexual adoptive parents, and highlight a need for services to support adoptive parents throughout the transition to parenthood to promote their well-being. (PsycINFO Database Record (c) 2014 APA, all rights reserved). PMID:24826826

  7. Adoption and Usage of Online Services in the Presence of Complementary Offline Services: Retail Banking

    OpenAIRE

    Anja Lambrecht; Katja Seim

    2006-01-01

    The availability and variety of online services has increased dramatically in recent years. Many questions remain, however, regarding patterns of online service use, consumer preferences when using online services, and how consumers substitute between equivalent online and offline services. Using an extensive data set of consumer adoption and usage of the online banking service of a major German bank, this paper analyzes consumers’ adoption and usage of online banking over the period August 2...

  8. Adoption of Internet Shopping: Cultural Considerations in India and Australia

    Directory of Open Access Journals (Sweden)

    Sujana Adapa

    2008-08-01

    Full Text Available The current research paper examines the adoption of internet shopping patterns exhibited by Indian women currently residing in India and Australia emphasizing on the prevailing cultural dimensions. A conceptual framework has been developed based on the theoretical background which links intention to shop over internet and Hofstede’s cultural dimensions to adoption of internet shopping. In order to test the stated hypotheses, the proposed relationships between the variables were empirically verified. A web based survey was employed by using online questionnaire as a research instrument and the respondents were approached by posting the questionnaire to various newsgroups. The results of the study reveal that intention of int ernet shopping as measured with the perceived attributes significantly influences the actual adoption of internet shopping. With regard to the prevailing cultural dimensions in the country of origin (India, the results obtained are as expected and signifi cantly influence the internet purchases. Where as with regard to the prevailing cultural dimensions in the country of residence (Australia, most of the results obtained are as predicted except for the dimension masculinity versus femininity.

  9. Adoption and non-adoption of mainstream formal banking systems amongst low income earners in the South Africa, Zambia and Zimbabwe

    Directory of Open Access Journals (Sweden)

    Mavhungu Abel Mafukata

    2015-03-01

    Full Text Available The majority of income earners of small-scale informal economic sectors in most developing regions of the world do not bank their earnings with mainstream formal banking institutions preferring to “bank” their income at their homes or not to bank at all. In addition, there is perception amongst mainstream formal banking institutions that low income earners were “unbankable” and therefore bad business. However, there is emerging literature opining that to the contrary, low income earners would provide a profitable niche market for mainstream formal banking institutions in the developing regions. In the Southern African Development Community (SADC, trends with regard adoption and non-adoption of mainstream formal banking systems amongst small income groups were mixed. This paper investigates adoption and non-adoption patterns of mainstream formal banking systems amongst small income earners in South Africa, Zambia and Zimbabwe. The results revealed that the informal cross-border traders who trade between Zambia and South Africa were good adopters of mainstream formal banking because the majority of them in these countries had already adopted mainstream formal banking through savings while only a few was left out of the system. However, there was a sharp contrast in Zambia where small income earners from the micro-finance adopting households were poorly banked with a huge majority which has been left out of the system. In Nyanga, Zimbabwe, of the respondents, only a few had adopted mainstream formal banking against the larger majority of non-adopters. The small income earners of the small-scale informal communal cattle farmers sub-sector in South Africa had 47.2% of its population adopting mainstream formal banking through savings whereas the majority 52.8% was left out. This paper concludes that adoption or non-adoption of mainstream formal banking systems patterns vary from region to region, and sector to sector even where income earners

  10. ADOPTION OF BIOSECURITY MEASURES BY LAYER SMALLHOLDERS

    Directory of Open Access Journals (Sweden)

    V.S. Lestari

    2014-10-01

    Full Text Available It was indicated that layer smallholders awareness of biosecurity was low. This paper aimed todetermine the level of adoption within the South Sulawesi layer smallholders of a range of standardbiosecurity measures. Sidenreng Rappang (Sidrap regency was chosen as a location of the research,because it was famous as a central of layer smallholders. Total sample was 60 respondents. The samplewas chosen through random sampling from two districts which were the most populous of layersmallholders, namely Baranti and Maritengae. Data were collected using structured questionnaires anddepth-interview. The data were tabulated and analysed using a simple method of scoring with regard totheir biosecurity status. The status of biosecurity was used to know the level of biosecurity adoption.Biosecurity status was obtained based on the adoption of biosecurity measures which consisted of 9stages: farm inputs, traffic onto farms, distance from sources of pathogens to shed, exposure of farm,biosecurity at farm boundary, biosecurity between farm boundary and shed, biosecurity at the shed door,traffic into the shed and susceptibility of the flock. Using adoption index, this research revealed thatbiosecurity adoption of layer smallholders in South Sulawesi was classified into a “partial adopter”.

  11. A Paradigm Shift: Adoption of Disruptive Learning Innovations in an ODL Environment: The Case of The University of South Africa

    OpenAIRE

    Blessing Mbatha

    2015-01-01

    The aim of this article is to shed some light on patterns of and major motives for the adoption of different types of disruptive learning innovations by Unisa academics. To realise the aim of the study, the following questions were addressed: What are the reasons for adopting disruptive learning innovations? What is the level of interaction with disruptive innovations? What training do Unisa academics require on disruptive innovations? A qualitative approach was adopted by conducting focus gr...

  12. The Cloud Adoption Toolkit: Addressing the Challenges of Cloud Adoption in Enterprise

    CERN Document Server

    Greenwood, David; Smith, James; Sommerville, Ian

    2010-01-01

    Cloud computing promises a radical shift in the provisioning of computing resource within enterprise. This paper: i) describes the challenges that decision-makers face when attempting to determine the feasibility of the adoption of cloud computing in their organisations; ii) illustrates a lack of existing work to address the feasibility challenges of cloud adoption in enterprise; iii) introduces the Cloud Adoption Toolkit that provides a framework to support decision-makers in identifying their concerns, and matching these concerns to appropriate tools/techniques that can be used to address them. The paper adopts a position paper methodology such that case study evidence is provided, where available, to support claims. We conclude that the Cloud Adoption Toolkit, whilst still under development, shows signs that it is a useful tool for decision-makers as it helps address the feasibility challenges of cloud adoption in enterprise.

  13. Attachment Behavior of Children Adopted Internationally at Six Months Post Adoption

    OpenAIRE

    Niemann, Sandra; Weiss, Sandra

    2011-01-01

    The purpose of this pilot study was to describe attachment behavior in children adopted internationally at 6 months post adoption. Twenty-two children and their adoptive mothers were observed at home for completion of an attachment measure. Mothers also completed a questionnaire on their child’s preadoption care. Comparisons of children low and high in attachment security suggested that low secure children showed predominantly ambivalent attachment behavior or that they were still in the proc...

  14. The late adopter scale: A measure of late adopters of technological innovations

    OpenAIRE

    Jahanmir, Sara F.; Lages, Luís Filipe

    2014-01-01

    Diffusion of Innovation is a topic of interest for researchers and practitioners. Although substantial research is conducted on user categories, researchers often focus on the first half of the curve, ignoring the late adopters. We conduct two studies to measure the attributes of late adopters. In our first study of mobile phone users, we develop the Late-Adopter Scale. We then test it on a sample of laptop users. This scale is multi-dimensional, presents nomological and discriminant valid...

  15. Stuck in the Adoption Funnel: The Effect of Interruptions in the Adoption Process on Usage

    OpenAIRE

    Anja Lambrecht; Katja Seim; Catherine Tucker

    2010-01-01

    Many firms have introduced Internet-based customer self-service applications such as online payments or brokerage services. Despite high initial sign-up rates, not all customers actually shift their dealings online. We investigate whether the multistage nature of the adoption process (an "adoption funnel") for such technologies can explain this low take-up. We use exogenous variation in events that possibly interrupt adoption, in the form of vacations and public holidays in different German s...

  16. Breaking the addiction to technology adoption.

    Science.gov (United States)

    Bryan, Stirling; Mitton, Craig; Donaldson, Cam

    2014-04-01

    A major driver of cost growth in health care is the rapid increase in the utilisation of existing technology and not simply the adoption of new technology. Health economists and their health technology assessment colleagues have become obsessed by technology adoption questions and have largely ignored 'technology management' questions. Technology management would include the life-cycle assessment of technologies in use, to assess their real-world performance; and monitoring of technology indication creep. A rebalancing of focus might serve to encourage a more self-critical and learning culture amongst those involved in technology evaluation analysis. Further, health economists and health technology assessment analysts could make a more significant contribution to system efficiency through rebalancing their efforts away from technology adoption questions towards technology management issues.

  17. Technology adoption subsidies. An experiment with managers

    International Nuclear Information System (INIS)

    We evaluate the impact of technology adoption subsidies on investment behavior in an individual choice experiment. In a laboratory setting professional managers are confronted with an intertemporal decision problem in which they have to decide whether or not to search for, and possibly adopt, a new technology. Technologies differ in the per-period benefits they yield, and their purchase price increases with the per-period benefits provided. We introduce a subsidy on the more expensive technologies (that also yield larger per-period benefits), and find that the subsidy scheme induces agents to search for and adopt these more expensive technologies even though the subsidy itself is too small to render these technologies profitable. We speculate that the result is driven by the positive connotation (affect) that the concept 'subsidy' invokes. (author)

  18. Breaking the addiction to technology adoption.

    Science.gov (United States)

    Bryan, Stirling; Mitton, Craig; Donaldson, Cam

    2014-04-01

    A major driver of cost growth in health care is the rapid increase in the utilisation of existing technology and not simply the adoption of new technology. Health economists and their health technology assessment colleagues have become obsessed by technology adoption questions and have largely ignored 'technology management' questions. Technology management would include the life-cycle assessment of technologies in use, to assess their real-world performance; and monitoring of technology indication creep. A rebalancing of focus might serve to encourage a more self-critical and learning culture amongst those involved in technology evaluation analysis. Further, health economists and health technology assessment analysts could make a more significant contribution to system efficiency through rebalancing their efforts away from technology adoption questions towards technology management issues. PMID:24590701

  19. Australian SMES' And E-Commerce adoption

    DEFF Research Database (Denmark)

    Scupola, Ada

    2010-01-01

    This chapter presents the results of a study on factors affecting adoption of e-commerce in small and medium size enterprises in Australia. The Tornatsky and Fleischer (1990) model is used in the investigation therefore the main group of factors taken into consideration are environmental, organiz......This chapter presents the results of a study on factors affecting adoption of e-commerce in small and medium size enterprises in Australia. The Tornatsky and Fleischer (1990) model is used in the investigation therefore the main group of factors taken into consideration are environmental......, organizational and technological. Top managers employed in five companies located in the area of Brisbane, Queensland have been interviewed. The results are partially in line with similar studies on e-commerce adoption in SMEs. However many differences are also found. For example this study found...

  20. Non-invasive prenatal diagnosis for homozygous α0-thalassemia based on paternal different SNP with allele-specific real-time fluorescence PCR: establishment and application%基于父源差异SNP位点的等位基因特异性实时荧光PCR方法建立及其在α0-地中海贫血无创产前诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    刘彦慧; 吴亚敏; 石少权; 娄季武; 马秋林; 何怡; 徐婉芳; 林洋洋; 周万军

    2012-01-01

    目的 建立并评价1种纯合α0-地中海贫血排除性无创产前诊断的等位基因特异性实时荧光PCR技术(AS-qPCR).方法 用PCR微测序技术,检测SEA高风险夫妇基因缺失区内9个SNP以确定双亲差异位点;然后以AS-qPCR技术检测孕妇血浆中父源差异SNP位点,判断父方是否将正常单倍型遗传给胎儿.结果 167个家系中,160个家系找到1个或多个有双亲差异的SNP位点,检测率为98.16%;94例检测到父源性正常等位基因SNP而免于创伤性产前诊断,检出率57.67%;所有家系的绒毛或羊水基因检测结果与本方法的符合率为100%.结论 采用AS-qPCR检测孕妇血浆游离DNA中双亲差异SNP位点的方法,在孕早期即可进行纯合α0-地中海贫血的排除性无创产前诊断,可使约50%高风险孕产妇免于创伤性产前诊断.%Objective To establish and evaluate an allele-specific real-time fluorescence PCR ( AS-qPCR) assay for non-invasive exclusive prenatal diagnosis of homozygous a -thalassemia. Methods Among 9 SNPs within the SEA deletion range, the different SNPs between parents were determined with PCR micro-sequencing technology. The paternal SNP was detected in maternal plasma DNA with AS-qPCR to determine whether the paternal normal haplotype had been inherited to the fetus. Results Among 167 families, 160 were found to have one or more parents-different SNPs with the detection rates over 98. 16%. The paternal normal SNPs were detected in 94 maternal plasma DNA. In this way, they avoided invasive prenatal diagnosis with a detection rate of 57.67%. These results were 100% coincidence with the results of CVS or amniocentesis. Conclusion Using AS-qPCR to detect the parents-difference SNPs in maternal plasma DNA in the early stage of gestation, is a non-invasive exclusive prenatal diagnosis of homozygous a?thalassemia. This method can make about 50% high-risk pregnant women to avoid invasive prenatal diagnosis.

  1. Adopting Deverbalization in Interpreting Chinese Political Neologisms

    Institute of Scientific and Technical Information of China (English)

    计霄雯

    2014-01-01

    Uniqueness of our political systems and tremendous changes brought about by reform and opening up are reflected in language use, so a boom of Chinese political neologisms is witnessed. Deverbalization, as the core concept of the Interpretive Theory should be adopted in interpreting such neologisms so as to convey the intended meaning of the speaker in a faithful and interpretive manner. This paper makes a tentative attempt at classifying those neologisms and elaborates through exemplification on adopting deverbalization in interpreting political neologisms, with a view to producing China English rather than Chinese English and facilitating political communication among countries.

  2. Adoption of Precision farming in Denmark

    DEFF Research Database (Denmark)

    Pedersen, Søren Marcus; Fountas, S.; Blackmore, S.;

    2003-01-01

    to demonstrate continuous yield improvements from Variable Rate Technology applications. The cost of gathering information, time consumption and incompatibility are barriers to adoption. Given teh uncertainty due to temporal variability, real time canopy management with N-sensors and weed detection is suggested......The adoption of precision farming (PF)in Denmark has been studied through two mail surveys, personal interviews and focus groups with farmers and other stakeholders. Farmers are in general optimistic about PF but it is difficult to verify the economic and environmental gains. It has been difficult...

  3. Adoption of GM Technology at Farm Level

    OpenAIRE

    Conor Keelan; Fiona Thorne; Paul Flanagan; Carol Newman; Ewen Mullins

    2008-01-01

    In this paper a probit model is used to assess the factors that will influence the decision of Irish farmers to adopt GM technology should they be given a choice in the near future of selecting between GM and non-GM varieties of crops. The theory underlying the model emanates from the wide literature on adoption and diffusions theory. The decision of whether or not to use new agricultural technologies has generally been found to be a function of specific features of the particular technology ...

  4. Retail industry adopting change : adaptation: automation: benefits

    OpenAIRE

    Ahmed, Nabeel

    2013-01-01

    This thesis contains the research on the key change adoptive agents/forces and the solu-tions to the world‟s rapidly growing and one of the most consumer facing industry. The trillions worth retail industries are undergoing the period of important restructuring inter-nally and externally. The author highlights the key factors that force the retail industry to adopt modern technologies for their daily business processes in order to be more competi-tive. The factors have been viewed in two pers...

  5. Smart Card Security; Technology and Adoption

    Directory of Open Access Journals (Sweden)

    Hamed Taherdoost

    2011-12-01

    Full Text Available Newly, smart card technology are being used in a number of ways around the world, on the otherhand, security has become significant in information technology, especially in those applicationinvolving data sharing and transactions through the internet. Furthermore, researches ininformation technology acceptance have identified the security as one of the factor that caninfluence on smart card adoption. This research is chiefly to study the security principals of smartcard and assess the security aspects’ affect on smart card technology adoption. In order toachieve this purpose, a survey was conducted among the 640 university students to measure theacceptance of smart card technology from security aspects.

  6. Technology adoption in nonrenewable resource management

    Energy Technology Data Exchange (ETDEWEB)

    Cunha-e-Sa, Maria A.; Balcao Reis, Ana [Universidade Nova de Lisboa, Faculdade de Economia, Campus de Campolide, PT-1099-032, Lisboa (Portugal); Roseta-Palma, Catarina [Departamento de Economia, ISCTE, Avenida das Forcas Armadas, PT-1649-026, Lisboa (Portugal)

    2009-03-15

    Technological change has played an important role in models of nonrenewable resource management, since its presence mitigates the depletion effect on extraction costs over time. We formalize the problem of a competitive nonrenewable resource extracting firm faced with the possibility of technology adoption. Based on a quadratic extraction cost function, our results show that the expected net benefits from adoption increase both with the size of the resource stock and with prices. A boundary that separates the region where expected net benefits are positive from the one where they are negative is derived. (author)

  7. Technology adoption in nonrenewable resource management

    International Nuclear Information System (INIS)

    Technological change has played an important role in models of nonrenewable resource management, since its presence mitigates the depletion effect on extraction costs over time. We formalize the problem of a competitive nonrenewable resource extracting firm faced with the possibility of technology adoption. Based on a quadratic extraction cost function, our results show that the expected net benefits from adoption increase both with the size of the resource stock and with prices. A boundary that separates the region where expected net benefits are positive from the one where they are negative is derived. (author)

  8. Taking a New Look at Empirical Models of Adoption: Average Treatment Effect Estimation of Adoption Rates and their Determinants

    OpenAIRE

    Diagne, Aliou

    2006-01-01

    This paper shows that the observed sample adoption rate does not consistently estimate the population adoption rate even if the sample is random. It is proved that instead the sample adoption rate is a consistent estimate of the population joint exposure and adoption rate, which does not inform about adoption per se. Likewise, it is shown that a model of adoption with observed adoption outcome as dependent variable and where exposure to the technology is not observed and controlled for cannot...

  9. 75 FR 68166 - National Adoption Month, 2010

    Science.gov (United States)

    2010-11-04

    ... From the Federal Register Online via the Government Publishing Office TD04NO10.016 [FR Doc. 2010... the United States of America the two hundred and thirty-fifth. (Presidential Sig.) [FR Doc. 2010-28117...; ] Proclamation 8597 of November 1, 2010 National Adoption Month, 2010 By the President of the United States...

  10. Overseas Adoptions in the U.S.

    Institute of Scientific and Technical Information of China (English)

    JerilynWatson; 刘保行

    2004-01-01

    Every year, thousands of Americans take a child into their home that was born in another country. Many husbands and wives in the USA want a child, but may not be able to have one. Or, such married couples may want to add an adopted (收养的) child to their other children. Single Americans may also want to become parents.

  11. Examining Teachers' Decisions to Adopt New Technology

    Science.gov (United States)

    Sugar, William; Crawley, Frank; Fine, Bethann

    2004-01-01

    This study examined teachers' beliefs about technology adoption as a reasoned, deliberate, intentional decision-making process, as reflected in Ajzen's (1985) Theory of Planned Behavior. Qualitative and quantitative data were collected from teachers in four schools located in the southeastern region of the United States. Overall results indicated…

  12. Personal Contacts and the Adoption of Innovations.

    Science.gov (United States)

    Alleyne, E. Patrick; Verner, Coolie

    A study undertaken among commercial strawberry growers in the Fraser Valley of British Columbia, Canada, sought to define the network of personal contacts as used by the farmers in obtaining information relevant to growing practices. Growers were divided into four adopter categories: laggards, late majority, early majority, and innovator-early…

  13. Wide-Scale Adoption of Photovoltaic Energy

    DEFF Research Database (Denmark)

    Yang, Yongheng; Enjeti, Prasad; Blaabjerg, Frede;

    2015-01-01

    of wide-scale penetration of single-phase PV systems in the distributed grid, disconnection under grid faults can contribute to 1) voltage flickers, 2) power outages, and 3) system instability. This article explores grid code modifications for a wide-scale adoption of PV systems in the distribution grid...

  14. Adoption of interorganisational ICT in primary care.

    NARCIS (Netherlands)

    Plomp, M.G.A.; Batenburg, R.S.; Verheij, R.A.

    2011-01-01

    Efficient and effective collaboration among health care providers is of great importance. Interorganisational ICT can enable and facilitate this collaboration, but the adoption of such information systems is still sparsely analysed. In this paper we describe the results of a survey among 49 GP pract

  15. Australian SMES' And E-Commerce adoption

    DEFF Research Database (Denmark)

    Scupola, Ada

    2010-01-01

    , organizational and technological. Top managers employed in five companies located in the area of Brisbane, Queensland have been interviewed. The results are partially in line with similar studies on e-commerce adoption in SMEs. However many differences are also found. For example this study found that the...

  16. The African Orphan Crisis and International Adoption

    Science.gov (United States)

    Roby, Jini L.; Shaw, Stacey A.

    2006-01-01

    The plight of Africa's AIDS orphans has reached crisis proportions, and the international community is beginning to mobilize at the family, community, national, and international levels. Despite these encouraging efforts, the response is inadequate, and increased attention and action are needed. The authors suggest that international adoption,…

  17. When to Tell Your Child About Adoption

    Science.gov (United States)

    ... Dynamics Adoption & Foster Care Communication & Discipline Types of Families Media Work & Play Getting Involved in Your Community Healthy Children > ... construct a more accurate "memory" of his original family, which of course is really just a fantasy about his first mother and father and how he came to be ...

  18. Environmental Justice and Green-Technology Adoption

    Science.gov (United States)

    Ong, Paul

    2012-01-01

    This paper presents an analysis of an environmental justice (EJ) program adopted by the South Coast Air Quality Management District (SCAQMD) as a part of its regulation to phase out a toxic chemical used by dry cleaners. SCAQMD provided financial incentives to switch early and gave establishments in EJ neighborhoods priority in applying for…

  19. Distributed PV Adoption - Sensitivity to Market Factors

    Energy Technology Data Exchange (ETDEWEB)

    Gagnon, Pieter; Sigrin, Ben

    2016-02-01

    NREL staff used the dSolar (distributed solar) model to forecast the adoption of distributed, behind-the-meter PV through the year 2050 for 9 different scenarios. The scenarios varied in their assumptions about a carbon tax, the cost of PV systems in the future, and what credit would be given for excess generation once current net metering policies expire.

  20. Adopting Learning Technologies: From Belief to Practice

    Science.gov (United States)

    Bothma, Cornelius H.; Cant, Michael C.

    2011-01-01

    A challenge faced by most heads of academic departments around the world is to manage the adoption and use of appropriate learning technologies in order to support the department's learning offerings to students. Earlier research undertaken by the authors revealed that lecturers within the Department of Marketing and Retail Management at the…

  1. Books on Adoption for Young Children: Looking at Language.

    Science.gov (United States)

    Schimmel, Nancy; Love, Susan

    1997-01-01

    Books can inform, reassure, and give young children the vocabulary to talk about adoption. This article presents and examines the language used to talk about adoption in eleven current children's books. Discusses surrogacy, adoption, "natural" parents, grief, "chosen-baby" stories, age at adoption, international adoption, foster children, and open…

  2. Effects of the history of adoption in the emotional adjustment of adopted adolescents.

    Science.gov (United States)

    Reppold, Caroline Tozzi; Hutz, Claudio Simon

    2009-11-01

    Since the decade of 1980, the model of stress and coping proposed for the assessment of vulnerability of adoptive families emphasizes that the emotional adjustment of those adopted is moderated by variables such as institutionalization, the manner and age at which the adoption was revealed, the change of first name, and the contact with the biological family. The objective of this study was to investigate the relationship of these variables to the perceived parenting style, mood, and self-esteem of the adopted adolescents. Participants in the study were 68 adolescents, between the ages of 14 and 15, adopted during infancy through judicial channels. The adolescents responded to a questionnaire about the history of adoption and to scales of Parenting Styles, Depression and Self-esteem. The main results indicated that the late revelation of adoption and the change of the first name are connected to higher levels of depression and low self-esteem and to more frequent perceptions of negligent or authoritarian parenting style. The contact with the biological family was frequently mentioned among those who perceived their parents as authoritative and presented the best indicator of mood and self-esteem. These findings were discussed in light of the necessity for multidisciplinary actions which can improve the psychological adaptation of the adopting families.

  3. Effects of the history of adoption in the emotional adjustment of adopted adolescents.

    Science.gov (United States)

    Reppold, Caroline Tozzi; Hutz, Claudio Simon

    2009-11-01

    Since the decade of 1980, the model of stress and coping proposed for the assessment of vulnerability of adoptive families emphasizes that the emotional adjustment of those adopted is moderated by variables such as institutionalization, the manner and age at which the adoption was revealed, the change of first name, and the contact with the biological family. The objective of this study was to investigate the relationship of these variables to the perceived parenting style, mood, and self-esteem of the adopted adolescents. Participants in the study were 68 adolescents, between the ages of 14 and 15, adopted during infancy through judicial channels. The adolescents responded to a questionnaire about the history of adoption and to scales of Parenting Styles, Depression and Self-esteem. The main results indicated that the late revelation of adoption and the change of the first name are connected to higher levels of depression and low self-esteem and to more frequent perceptions of negligent or authoritarian parenting style. The contact with the biological family was frequently mentioned among those who perceived their parents as authoritative and presented the best indicator of mood and self-esteem. These findings were discussed in light of the necessity for multidisciplinary actions which can improve the psychological adaptation of the adopting families. PMID:19899647

  4. Envisaging the Adoption Process to Strengthen Gay- and Lesbian-Headed Families: Recommendations for Adoption Professionals

    Science.gov (United States)

    Matthews, John D.; Cramer, Elizabeth P.

    2006-01-01

    Although a growing number of child placement agencies are serving lesbians and gay men, a dearth of literature exists for adoption agency policies and practices related to working with this population. This article explores the unique characteristics and strengths of prospective gay and lesbian adoptive parents throughout each of the three phases…

  5. Active Drivers of Adoption of Internet Banking

    Directory of Open Access Journals (Sweden)

    Pankaj Yadav

    2016-05-01

    Full Text Available Application of internet by banking sector in India has changed the definition of banking completely. The management of different banks has been making concrete efforts to facilitate the acceptance of i-banking by bringing out its benefits to the customers. This paper identifies the active factors that influence customers intention regarding use of internet banking in Himachal Pradesh  Data was collected from a sample of 120 respondents through a well structured questionnaire. The data was collected from Government employees of Shimla district of Himachal Pradesh by asking close ended questions regarding their attitude towards i-banking and their future intention about its use . For analysing and interpreting data in the present study, basic statistical tools, chi-square test (test of independence and logistical regression analysis was used with the help of SPSS 16.0 version. Out of seven factors i.e., perceived risk, perceived usefulness,  perceived quality of service, Perceived cost, Perceived ease of use, trust and awareness, three significant factors i.e., perceived risk, perceived usefulness  and perceived quality of service have been identified that actively influence future adoption intention to adopt internet banking . Further the study exhibited that perceived usefulness and perceived quality of service positively influence the intention to adopt i-banking while perceived risk negatively affect the future adoption intention of i-banking. This  model has the overall predictability of classifying 76.7 % cases correctly and exhibited that 87.2 % customers are classified for adopting i-banking service whereas 57.1 % customers for not having intention to use it in future

  6. A Disciplined Approach to Adopting Agile Practices: The Agile Adoption Framework

    CERN Document Server

    Sidky, Ahmed; Bohner, Shawn

    2007-01-01

    Many organizations aspire to adopt agile processes to take advantage of the numerous benefits that it offers to an organization. Those benefits include, but are not limited to, quicker return on investment, better software quality, and higher customer satisfaction. To date however, there is no structured process (at least in the public domain) that guides organizations in adopting agile practices. To address this problem we present the Agile Adoption Framework. The framework consists of two components: an agile measurement index, and a 4-Stage process, that together guide and assist the agile adoption efforts of organizations. More specifically, the agile measurement index is used to identify the agile potential of projects and organizations. The 4-Stage process, on the other hand, helps determine (a) whether or not organizations are ready for agile adoption, and (b) guided by their potential, what set of agile practices can and should be introduced.

  7. Dynamics of gene silencing during X inactivation using allele-specific RNA-seq

    NARCIS (Netherlands)

    Marks, Hendrik; Kerstens, Hindrik H D; Barakat, Tahsin Stefan; Splinter, Erik; Dirks, René A M; van Mierlo, Guido; Joshi, Onkar; Wang, Shuang-Yin; Babak, Tomas; Albers, Cornelis A; Kalkan, Tüzer; Smith, Austin; Jouneau, Alice; de Laat, Wouter; Gribnau, Joost; Stunnenberg, Hendrik G

    2015-01-01

    BACKGROUND: During early embryonic development, one of the two X chromosomes in mammalian female cells is inactivated to compensate for a potential imbalance in transcript levels with male cells, which contain a single X chromosome. Here, we use mouse female embryonic stem cells (ESCs) with non-rand

  8. Allele Specific Expression of MICA Variants in Human Fibroblasts Suggests a Pathogenic Mechanism.

    Science.gov (United States)

    Shi, Chunhua; Li, Hongye; Couturier, Jacob P; Yang, Karen; Guo, Xinjian; He, Dongyi; Lewis, Dorothy E; Zhou, Xiaodong

    2015-01-01

    The major histocompatibility complex class I chain-related gene A (MICA) is involved in immune responses of both nature killer (NK) cells and subsets of T cells with its receptor NKG2D. MICA is highly polymorphic in sequence which leads to MICA protein variants with distinct features. Specific polymorphisms of MICA have been associated with inflammatory diseases, including ankylosing spondylitis (AS), ulcerative colitis (UC) and Behçet's disease. Studies herein characterize expression features of three MICA variants including MICA*008, a common variant in general population, and *MICA*007 and *019, which are associated with susceptibility to inflammatory diseases. MICA*019 was highly expressed on the surface of fibroblasts whereas expression of MICA*007 was the lowest in the culture supernatant. MICA*008 had low cell surface expression but was the only MICA allele in which exosomal material was detected. Surface or membrane-bound MICA activates NKG2D-mediated cytotoxicity, whereas soluble and exosomal MICAs down-regulate NKG2D. Therefore, comparisons of these three MICA variants in fibroblasts provides insight into understanding how MICA associated immune responses could be regulated to influence levels of inflammation.

  9. A four-element based transposon system for allele specific tagging in plants – Theoretical considerations

    Indian Academy of Sciences (India)

    Sanjay Phogat; Pradeep Kumar Burma; Deepak Pental

    2000-03-01

    The two-element transposon constructs, utilizing either Ac/Ds or Spm/dSpm, allow random tagging of genes in heterologous model species, but are inadequate for directed tagging of specific alleles of agronomic importance. We propose the use of Ac/Ds in conjunction with Spm/dSpm to develop a four-element system for directed tagging of crop-specific alleles. The four-element based construct would include both Ds and dSpm along with relevant marker genes and would function in two steps. In the first step dSpm(Ds) stocks (a minimum of two) would be crossed to a line containing transposases of Spm and unlinked integrations would be selected from segregating population by the use of a negative selection marker to develop stocks representing integration of dSpm(Ds) at a large number of locations in the genome. Selections would be made for a line in which dSpm(Ds) shows partial or complete linkage to the allele of interest. In the second step selected line would be crossed to a line containing Ac transposase to induce transpositions of Ds element to linked sites thereby exploiting the natural tendency of Ds element to jump to linked sites. Unlinked jumps of dSpm(Ds) and linked jumps of Ds could be monitored by appropriate marker genes. The proposed model would allow tagging of allele of interest in chromosome addition lines and also help in the efficient use of genic male sterility systems for hybrid seed production by tightly marking the fertility restorer gene with a negative selection marker.

  10. Allele-specific up-regulation of FGFR2 increases susceptibility to breast cancer.

    Directory of Open Access Journals (Sweden)

    Kerstin B Meyer

    2008-05-01

    Full Text Available The recent whole-genome scan for breast cancer has revealed the FGFR2 (fibroblast growth factor receptor 2 gene as a locus associated with a small, but highly significant, increase in the risk of developing breast cancer. Using fine-scale genetic mapping of the region, it has been possible to narrow the causative locus to a haplotype of eight strongly linked single nucleotide polymorphisms (SNPs spanning a region of 7.5 kilobases (kb in the second intron of the FGFR2 gene. Here we describe a functional analysis to define the causative SNP, and we propose a model for a disease mechanism. Using gene expression microarray data, we observed a trend of increased FGFR2 expression in the rare homozygotes. This trend was confirmed using real-time (RT PCR, with the difference between the rare and the common homozygotes yielding a Wilcox p-value of 0.028. To elucidate which SNPs might be responsible for this difference, we examined protein-DNA interactions for the eight most strongly disease-associated SNPs in different breast cell lines. We identify two cis-regulatory SNPs that alter binding affinity for transcription factors Oct-1/Runx2 and C/EBPbeta, and we demonstrate that both sites are occupied in vivo. In transient transfection experiments, the two SNPs can synergize giving rise to increased FGFR2 expression. We propose a model in which the Oct-1/Runx2 and C/EBPbeta binding sites in the disease-associated allele are able to lead to an increase in FGFR2 gene expression, thereby increasing the propensity for tumour formation.

  11. Allele-specific silencing of mutant Huntington’s disease gene

    OpenAIRE

    Zhang, Yu; Engelman, Joshua; Friedlander, Robert M.

    2009-01-01

    Huntington’s disease (HD) is an autosomal-dominant neurodegenerative disorder caused by a poly-glutamine expansion in huntingtin, the protein encoded by the HD gene. PolyQ-expanded huntingtin is toxic to neurons, especially the medium spiny neurons (MSNs) of the striatum. At the same time, wild-type huntingtin has important -- indeed essential -- protective functions. Any effective molecular therapy must preserve the expression of wild-type huntingtin, while silencing the mutant allele. We hy...

  12. Sequenza: allele-specific copy number and mutation profiles from tumor sequencing data

    DEFF Research Database (Denmark)

    Favero, Francesco; Joshi, Tejal; Marquard, Andrea Marion;

    2015-01-01

    Background : Exome or whole genome deep sequencing of tumor DNA along with paired normal DNA can potentially provide a detailed picture of the somatic mutations that characterize the tumor. However, analysis of such sequence data can be complicated by the presence of normal cells in the tumor spe...

  13. Allele specific gain-of-function activity of p53 mutants in lung cancer cells

    OpenAIRE

    Vaughan, Catherine A.; Frum, Rebecca; Pearsall, Isabella; Singh, Shilpa; Windle, Brad; Yeudall, Andrew; Deb, Swati P.; Deb, Sumitra

    2012-01-01

    p53 mutations are mostly single amino acid changes resulting in expression of a stable mutant protein with “gain of function” (GOF) activity having a dominant oncogenic role rather than simple loss of function of wild-type p53. Knock-down of mutant p53 in human lung cancer cell lines with different endogenous p53 mutants results in loss of GOF activity as shown by lowering of cell growth rate. Two lung cancer cell lines, ABC1 and H1437 carrying endogenous mutants p53–P278S and –R267P, both sh...

  14. Allele-specific regulation of MTTP expression influences the risk of ischemic heart disease

    DEFF Research Database (Denmark)

    Aminoff, Anna; Ledmyr, Helena; Thulin, Petra;

    2010-01-01

    Promoter polymorphisms in microsomal triglyceride transfer protein (MTTP) have been associated with decreased plasma lipids but an increased risk for ischemic heart disease (IHD), indicating that MTTP influences the susceptibility for IHD independent of plasma lipids. The objective of this study...... was to characterize the functional promoter polymorphism in MTTP predisposing to IHD and its underlying mechanism. Use of pyrosequencing technology revealed that presence of the minor alleles of the promoter polymorphisms -493G>T and -164T>C result in lower transcription of MTTP in vivo in the heart, liver...... including a total of 544 IHD patients and 544 healthy control subjects. We concluded that carriers of the minor -164C allele have lower expression of MTTP in the heart, mediated at least partly by the transcription factor CCAAT/enhancer binding protein, and that reduced concentration of MTTP...

  15. Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels

    Energy Technology Data Exchange (ETDEWEB)

    Doktycz, M.J.; Weber, J.L. (Marshfield Medical Research Foundation)

    2000-06-01

    The first Specific Aim for this grant was to identify and characterize an average of 500 human insertion/deletion polymorphisms per grant year (1500 total). This task was carried out entirely at MMRF. They substantially exceeded this goal by confirming about 2,300 diallelic indels. Complete characterization information for these polymorphisms is available from the Marshfield web site. A manuscript describing results for the first 2,000 diallelic indels was published earlier this year in the American Journal of Human Genetics. The Second Specific Aim of the grant was to investigate and develop improved methods for analysis of diallelic polymorphisms using miniaturized DNA arrays. The initial genotyping technology efforts focused on various hybridization and extension protocols with oligo arrays on flow-through channel glass. Channel glass is a porous material that permits reagents to be passed through the arrays. They devoted roughly 19 months at the beginning of the grant in pursuit of this methodology, but for various technological reasons, progress was limited.

  16. SNP detection in mRNA in living cells using allele specific FRET probes.

    Directory of Open Access Journals (Sweden)

    Liya Dahan

    Full Text Available Live mRNA detection allows real time monitoring of specific transcripts and genetic alterations. The main challenge of live genetic detection is overcoming the high background generated by unbound probes and reaching high level of specificity with minimal off target effects. The use of Fluorescence Resonance Energy Transfer (FRET probes allows differentiation between bound and unbound probes thus decreasing background. Probe specificity can be optimized by adjusting the length and through use of chemical modifications that alter binding affinity. Herein, we report the use of two oligonucleotide FRET probe system to detect a single nucleotide polymorphism (SNP in murine Hras mRNA, which is associated with malignant transformations. The FRET oligonucleotides were modified with phosphorothioate (PS bonds, 2'OMe RNA and LNA residues to enhance nuclease stability and improve SNP discrimination. Our results show that a point mutation in Hras can be detected in endogenous RNA of living cells. As determined by an Acceptor Photobleaching method, FRET levels were higher in cells transfected with perfect match FRET probes whereas a single mismatch showed decreased FRET signal. This approach promotes in vivo molecular imaging methods and could further be applied in cancer diagnosis and theranostic strategies.

  17. DQB1*06:02 allele-specific expression varies by allelic dosage, not narcolepsy status

    DEFF Research Database (Denmark)

    Weiner Lachmi, Karin; Lin, Ling; Kornum, Birgitte Rahbek;

    2012-01-01

    The association of narcolepsy-cataplexy, a sleep disorder caused by the loss of hypocretin/orexin neurons in the hypothalamus, with DQA1*01:02-DQB1*06:02 is one of the tightest known single-allele human leukocyte antigen (HLA) associations. In this study, we explored genome-wide expression in per...

  18. Rapid identification of capybara (Hydrochaeris hydrochaeris through allele-specific PCR

    Directory of Open Access Journals (Sweden)

    Flávio Henrique-Silva

    2005-07-01

    Full Text Available The capybara is the largest rodent in the world and is widely distributed throughout Central and South America.  It is an animal of economic interest due to the pleasant flavor of its meat and higher protein content in comparison  to beef and pork meat.  The hide, hair and fat also have economic advantages. Thus,  as an animal with such high economic potential, it is the target of hunters, even though  hunting capybara is prohibited by law in Brazil.   Due to their  similarities,  capybara meat  is easily confused with  pork  meat.   This  occurs  upon  the apprehension of the  meat  from hunters, as well as in some restaurants that serve capybara meat that was slaughtered clandestinely. In both cases, when the meat is confiscated, those responsible for the crimes claim it is pork meat,  hindering  the enforcement of the law. A practical  course was ministered  to undergraduate biology students enrolled in the elective course Introduction to Genetic  Engineering  at Federal  University  of Sao Carlos (UFSCar, Sao Paulo  State, Brazil.  The  objective  of the  course was to establish  and  apply  a Polymerase  Chain  Reaction  (PCR assay to identify capybara meat and discriminate it in relation  to other types of meat,  including pork. Primers  were designed based  on 12S rRNA,  transthyretin and  growth  hormone  receptor  genes.  The primers generated  capybara specific fragments  of approximately 220, 290 and 330 bp for transthyretin,12S rRNA  and  growth  hormone  receptor,  respectively.   The  duplexes  developed  in the  present work can be used effectively to discriminate capybara meat  from other  animals,  contributing to combating predatory capybara hunting. The results were extensively discussed and the students have contributed to written a paper  to be submitted to a publication.

  19. Lack of allele-specific efficacy of a bivalent AMA1 malaria vaccine

    Directory of Open Access Journals (Sweden)

    Ellis Ruth D

    2010-06-01

    Full Text Available Abstract Background Extensive genetic diversity in vaccine antigens may contribute to the lack of efficacy of blood stage malaria vaccines. Apical membrane antigen-1 (AMA1 is a leading blood stage malaria vaccine candidate with extreme diversity, potentially limiting its efficacy against infection and disease caused by Plasmodium falciparum parasites with diverse forms of AMA1. Methods Three hundred Malian children participated in a Phase 2 clinical trial of a bivalent malaria vaccine that found no protective efficacy. The vaccine consists of recombinant AMA1 based on the 3D7 and FVO strains of P. falciparum adjuvanted with aluminum hydroxide (AMA1-C1. The gene encoding AMA1 was sequenced from P. falciparum infections experienced before and after immunization with the study vaccine or a control vaccine. Sequences of ama1 from infections in the malaria vaccine and control groups were compared with regard to similarity to the vaccine antigens using several measures of genetic diversity. Time to infection with parasites carrying AMA1 haplotypes similar to the vaccine strains with respect to immunologically important polymorphisms and the risk of infection with vaccine strain haplotypes were compared. Results Based on 62 polymorphic AMA1 residues, 186 unique ama1 haplotypes were identified among 315 ama1 sequences that were included in the analysis. Eight infections had ama1 sequences identical to 3D7 while none were identical to FVO. Several measures of genetic diversity showed that ama1 sequences in the malaria vaccine and control groups were comparable both at baseline and during follow up period. Pre- and post-immunization ama1 sequences in both groups all had a similar degree of genetic distance from FVO and 3D7 ama1. No differences were found in the time of first clinical episode or risk of infection with an AMA1 haplotype similar to 3D7 or FVO with respect to a limited set of immunologically important polymorphisms found in the cluster 1 loop of domain I of AMA1. Conclusion This Phase 2 trial of a bivalent AMA1 malaria vaccine found no evidence of vaccine selection or strain-specific efficacy, suggesting that the extreme genetic diversity of AMA1 did not account for failure of the vaccine to provide protection.

  20. Allele-specific expression of the low density lipoprotein receptor gene

    Energy Technology Data Exchange (ETDEWEB)

    Minnich, A.; Lussier-Cacan, S.; Roy, M. [Clincial Research Institute of Montreal, Quebec (Canada)

    1994-09-01

    Approximately 60% of familial hypercholesterolemia (FH) in French Canadians is due to a > 10 kb deletion of the promoter region of the gene encoding the low density lipoprotein (LDL) receptor (LDL-R), allowing determination of the influence of a single LDL-R allele on phenotypic expression of FH. Normal allele haplotypes of approximately 250 heterozygotes were determined with 7 RFLPs. In vitro maximal LDL-R activity of blood lymphocytes from a subset of approximately 150 heterozygotes, measured by immunocytofluorometry, was significantly higher (20 to 30%) in subjects with LDL-R normal allele haplotype G (n=11), and O (n=7) compared to the most frequent haplotype F (n=43), while no differences were observed among F, E (n=11), and the 2 other most prevalent haplotypes (n=43). LDL-R mRNA in these lymphocytes was significantly elevated 2.3-, 1.7-, and 1.8- fold, in G, O, and E, respectively, compared to F, while no significant differences were apparent between F and the other two most frequent haplotyes. Large interindividual variability in lymphocyte LDL-R mRNA levels and activity was observed even among subjects with the same LDL-R normal allele haplotype. However, maximally induced lymphocyte LDL-R mRNA levels correlated poorly with levels measured in freshly isolated cells (n=14). Relative to haplotype F (n=47 women (W), 39 men (M)), mean plasma LDL cholesterol levels adjusted for age and apolipoprotein E genotype were 5-10% lower in men and women with haplotypes G (n=16 W, 12 M) and O (n=8 W, 6 M), and 20% lower in 7 W with haplotype E. These results suggest that (1) normal LDL-R allele haplotype G and O may contain sequence variations which confer relatively high gene expression and (2) environmental and genetic influences other than the LDL-R gene contribute substantially to variability in LDL-R expression and plasma LDL cholesterol levels in French Canadian FH heterozygotes.

  1. Genome-wide survey of allele-specific splicing in humans

    OpenAIRE

    Nembaware, Victoria; Lupindo, Bukiwe; Schouest, Katherine; Spillane, Charles; Scheffler, Konrad; Seoighe, Cathal

    2008-01-01

    Background Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a mutation...

  2. Genome-wide survey of allele-specific splicing in humans

    OpenAIRE

    Scheffler Konrad; Spillane Charles; Schouest Katherine; Lupindo Bukiwe; Nembaware Victoria; Seoighe Cathal

    2008-01-01

    Abstract Background Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a...

  3. Ethnic and adoption attitudes among Guatemalan University students

    OpenAIRE

    Gibbons,Judith L.; González-Oliva, Ana Gabriela; Mylonas, Kostas

    2015-01-01

    Intercountry adoptions from Guatemala were highly controversial, because of the large numbers of children being adopted to the USA, along with evidence of corruption and child theft. Since the implementation of the Hague Convention on Intercountry Adoption in 2008, Guatemala’s central authority for adoption has prioritized domestic placements for children over intercountry adoption. A possible attitudinal barrier to domestic adoption in Guatemala—negative attitudes and prejudice against Indig...

  4. MODELS OF TECHNOLOGY ADOPTION: AN INTEGRATIVE APPROACH

    Directory of Open Access Journals (Sweden)

    Andrei OGREZEANU

    2015-06-01

    Full Text Available The interdisciplinary study of information technology adoption has developed rapidly over the last 30 years. Various theoretical models have been developed and applied such as: the Technology Acceptance Model (TAM, Innovation Diffusion Theory (IDT, Theory of Planned Behavior (TPB, etc. The result of these many years of research is thousands of contributions to the field, which, however, remain highly fragmented. This paper develops a theoretical model of technology adoption by integrating major theories in the field: primarily IDT, TAM, and TPB. To do so while avoiding mess, an approach that goes back to basics in independent variable type’s development is proposed; emphasizing: 1 the logic of classification, and 2 psychological mechanisms behind variable types. Once developed these types are then populated with variables originating in empirical research. Conclusions are developed on which types are underpopulated and present potential for future research. I end with a set of methodological recommendations for future application of the model.

  5. Mobile ICT Acceptance in Late Adopter Countries

    DEFF Research Database (Denmark)

    Gimpel, Gregory; Sudzina, Frantisek; Petrovcikova, Katarina

    2014-01-01

    Despite the rapid global diffusion of the smartphone, some countries have experienced much slower uptake of the technology. The low smartphone penetration within Slovakia provides the opportunity to explore what drives smartphone use in late majority countries. Slovakia is a central European nation...... and part of the Eurozone. It has advanced telecommunications infrastructure and is subject to the same telecommunications regulations as other EU members. While neighbours have high smartphone penetration, Slovakia is a late majority adopter. This study uses Triandis’ theory of interpersonal behavior...... to investigate the question: What drives the use of smartphones in late majority countries? By studying the differences between current and potential smartphone users, the study revisits Karahanna et al.’s research question: Do potential adopters and users of IT hold the same behavioral and normative beliefs...

  6. Evaluating IPv6 Adoption in the Internet

    Science.gov (United States)

    Colitti, Lorenzo; Gunderson, Steinar H.; Kline, Erik; Refice, Tiziana

    As IPv4 address space approaches exhaustion, large networks are deploying IPv6 or preparing for deployment. However, there is little data available about the quantity and quality of IPv6 connectivity. We describe a methodology to measure IPv6 adoption from the perspective of a Web site operator and to evaluate the impact that adding IPv6 to a Web site will have on its users. We apply our methodology to the Google Web site and present results collected over the last year. Our data show that IPv6 adoption, while growing significantly, is still low, varies considerably by country, and is heavily influenced by a small number of large deployments. We find that native IPv6 latency is comparable to IPv4 and provide statistics on IPv6 transition mechanisms used.

  7. Physician Agency and Adoption of Generic Pharmaceuticals

    OpenAIRE

    Toshiaki Iizuka

    2012-01-01

    I examine physician agency in health care services in the context of the choice between brand-name and generic pharmaceuticals. I examine micro-panel data from Japan, where physicians can legally make profits by prescribing and dispensing drugs. The results indicate that physicians often fail to internalize patient costs, explaining why cheaper generics are infrequently adopted. Doctors respond to markup differentials between the two versions, indicating another agency problem. However, gener...

  8. Adoption dynamics: sequential or synchronous modelling

    OpenAIRE

    Hardouin, Cécile

    2012-01-01

    This paper deals with the choice of dynamics in spatial simulation and modelling. In economical context, N agents choose between two technological standards according to a local assignment rule. The adoption dynamics is sequential if the choices are made one after the other; it is synchronous or partially synchronous if all or some part of the agents choose simultanously. This paper points out differences between the three dynamics, especially in their evolution.

  9. A Conceptual Model for Agile Practices Adoption

    OpenAIRE

    Silveira Campanelli, Amadeu; Silva Parreiras, Fernando

    2014-01-01

    The software development industry has been adopting agile methods and practices instead of traditional software development methods because they are more flexible and can bring benefits such as handling requirements changes, productivity gains, frequent releases and business strategy focus. This work proposes a model for agile process data representation to show the relationships between agile components (principles, methods, practices, techniques, roles and work products) to allow organizati...

  10. LEARNING STYLES ADOPTED BY MEDICAL STUDENTS

    OpenAIRE

    Chinmay Shah; Naisargi Joshi; Mehta, H. B.; P.A.Gokhle

    2011-01-01

    Learning results in gain of knowledge, skills and attitudes. Some like to learn by seeing, some by hearing and some by demonstration. Learning style influences the retention of information and depth of comprehension. Understanding their preferred learning styles as visual, auditory, read-write or kinesthetic learners will help improve the teaching methods adopted. Role of the educator necessitates making the most of each teaching opportunity by understanding the characteristics of the learnin...

  11. Customer Adoption of Internet Banking in Mauritius

    OpenAIRE

    N. Mamode Khan; N. Emmambokus

    2011-01-01

    Internet banking offers many benefits but little research has been done about its acceptance in Mauritius. This paper aims at assessing the factors that contribute to the adoption of internet banking in Mauritius. To support our arguments, we use a logistic regression model based on a sample survey to analyze the factors that influence internet banking in Mauritius. We conclude that factors such as age, income, service usefulness, risk factor, checking account frequency and internet location ...

  12. Cereal production and technology adoption in Ethiopia:

    OpenAIRE

    Yu, Bingxin; Nin-Pratt, Alejandro; Funes, José; Gemessa, Sinafikeh Asrat

    2011-01-01

    The Ethiopian government has been promoting a package-driven extension that combines credit, fertilizers, improved seeds, and better management practices. This approach has reached almost all farming communities, representing about 2 percent of agricultural gross domestic product in recent years. This paper is the first to look at the extent and determinants of the adoption of the fertilizer-seed technology package promoted in Ethiopia using nationally representative data from the Central Sta...

  13. SMS advertisements\\\\\\' adoption among Tehran mobile users

    Directory of Open Access Journals (Sweden)

    Manizheh Haghighinasab

    2014-05-01

    Full Text Available Mobile marketing and SMS (short message services advertising in particular, have been recently used. Even though SMS-based advertising is inexpensive, interactive, and able to yield a high response rate, middle or big size companies do not use it in Iran. For as much as its adoption was unclear, this study was organized to find it out. Since the most populated city in our country, Tehran, is a good multicultural instance to study in many fields, this research has aimed at its citizens' intention to use SMS advertisement. The statistical populations included are mobile users, who came to Mobile Customer Service located in north, south, west, east and centre of Tehran. From which 384 were considered as samples. Technology Acceptance Model (TAM was used for making hypothesizes. Survey's data was collected by questionnaire with cluster and random sampling. Collected data was analyzed regarding to the method: Structural Equation Modeling (SEM in order to do which, the technique Partial List Squares (PLS were used. The results revealed that: despite the lack of adoption for the SMS advertising among mobile users in Tehran, there could be chances to improve it by better perceived usefulness as well as its ease of use. Innovativeness of the people and their trust has indirect impact on their intention to use. Moreover, getting prior permission, making a profile, and individualizing the context with richness of information and entertainers are recommended. Using the new generation 3G and shortcuts will be able to improve SMS advertisement's adoption as well.

  14. Understanding Physicians' Adoption of Health Clouds

    Directory of Open Access Journals (Sweden)

    Tatiana Ermakova

    2015-01-01

    Full Text Available Recently proposed health applications are able to e nforce essential advancements in the healthcare sector. The design of these innovative s olutions is often enabled through the cloud computing model. With regards to this technology, h igh concerns about information security and privacy are common in practice. These concerns with respect to sensitive medical information could be a hurdle to successful adoptio n and consumption of cloud-based health services, despite high expectations and interest in these services. This research attempts to understand behavioural intentions of healthcare pro fessionals to adopt health clouds in their clinical practice. Based on different established t heories on IT adoption and further related theoretical insights, we develop a research model a nd a corresponding instrument to test the proposed research model using the partial least squ ares (PLS approach. We suppose that healthcare professionals’ adoption intentions with regards to health clouds will be formed by their outweighing two conflicting beliefs which are performance expectancy and medical information security and privacy concerns associate d with the usage of health clouds. We further suppose that security and privacy concerns can be explained through perceived risks.

  15. THE FORESIGHTS OF THE ADOPTION OF EURO

    Directory of Open Access Journals (Sweden)

    Claudia Loredana Juncu

    2012-06-01

    Full Text Available Even if the Euro critics showed the warning that the change of the national currency brings a loose of the control and the decision power about the monetary problems and the flexibility of the decisions, many consider that the adoption of the single currency would allow us to protect the economies from the external shocks. We have to eliminate the uncertainty, the high costs and we have to be able to avoid and / or manage the monetary fluctuations especially in situations like today’s economic crisis. One of the effects the crisis has created throughout the European Union and especially in Romania is connected with the return back in the country of the population that works in Western Europe and the reflux of money it implies. The structural problems of the Romanian economy that creates this workforce dynamic is to continue to affect the Euro adoption plan until significant solutions will be addressed in order to maintain the workforce in the country.Europe is facing a dilemma. For now there is a significant distrust in Euro. Romania needs to acknowledge the fact that a single European currency allows first of all the overpass of the actual internal fragmentation of the market and so to strengthen the national position in relation with the international. Romania can still be optimistic about the objective of adopting Euro if an efficient financial policy will be implemented.

  16. Broadband in Schools: Effects on Student Performance and Spillovers for Household Internet Adoption

    Science.gov (United States)

    Belo, Rodrigo

    2012-01-01

    This work comprises studies on the effects of broadband Internet in schools at three different levels: student performance, household Internet adoption, and individual computer and Internet use patterns and skill acquisition. I focus in the case of Portugal, where by 2006 the Portuguese government had completed a major initiative that upgraded the…

  17. Measuring sustainable broadband adoption: An innovative approach to understanding broadband adoption and use

    OpenAIRE

    Larose, Robert; De Maagd, Kurt; Chew, Han Ei; Tsai, Hsin-yi Sandy; Steinfield, Charles; Wildman, Steven S; Bauer, Johannes M.

    2012-01-01

    Efforts to promote sustainable broadband Internet adoption urge new attention to the classic diffusion of innovations paradigm. For this study, innovation attributes were reconceptualized following Social Cognitive Theory (SCT). In a sample of inner-city residents, the model accounted for 36% of the variance in intentions to adopt broadband technology and services, primarily from the SCT variables of expected outcomes and self-efficacy. Prior habitual use of the Internet was also a predictor....

  18. Beyond Technology Adoption: Homeowner Satisfaction with Newly Adopted Residential Heating Systems

    OpenAIRE

    Michelsen , Carl Christian; Madlener, Reinhard

    2015-01-01

    In this paper we study homeowner satisfaction with respect to innovative residential heating systems. In particular, we focus on the role of attributes of the home, homeowners’ socio-demographic characteristics, RHS-related knowledge, and adoption motivations. For this purpose, we apply a linear regression model on a dataset obtained from a survey among homeowners in Germany (N=2,135) that had adopted a RHS shortly before the survey was conducted. Moreover, we investigate differences between ...

  19. Motives behind software adoption in Finnish accounting firms: Technology adoption motives in two case firms

    OpenAIRE

    Laitinen, Aki

    2014-01-01

    Objectives of the Study: The objective of this study is to better understand the factors affecting software adoption decision-making in Finnish accounting firms. These firms are, more often than not, smaller organisations and same systems are used for long periods of time. As efforts to improve data transfer standards and processes progress at the regulatory level, it is curious to see how technology adoption decision-making takes place at a firm level. Academic background and method...

  20. Pig design patterns

    CERN Document Server

    Pasupuleti, Pradeep

    2014-01-01

    Pig makes Hadoop programming simple, intuitive, and fun to work with. It removes the complexity from Map Reduce programming by giving the programmer immense power through its flexibility. What used to be extremely lengthy and intricate code written in other high level languages can now be written in almost one tenth of the size using its easy to understand constructs. Pig has proven to be the easiest way to learn how to program Hadoop clusters, as evidenced by its widespread adoption. This comprehensive guide enables readers to readily use design patterns to simplify the creation of complex da

  1. 27 CFR 17.125 - Adoption of formulas and processes.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Adoption of formulas and... NONBEVERAGE PRODUCTS Formulas and Samples § 17.125 Adoption of formulas and processes. (a) Adoption of... of adoption with the appropriate TTB officer. The notice shall be filed with the first claim...

  2. 27 CFR 24.127 - Adoption of formulas.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Adoption of formulas. 24... Establishment § 24.127 Adoption of formulas. The adoption of approved formulas by a successor proprietor will be... formulas for adoption by formula number, name of product, and date of approval. The application...

  3. 27 CFR 19.187 - Adoption of formulas.

    Science.gov (United States)

    2010-04-01

    ... 27 Alcohol, Tobacco Products and Firearms 1 2010-04-01 2010-04-01 false Adoption of formulas. 19... After Original Qualification § 19.187 Adoption of formulas. (a) Forms 5110.38. The adoption by a... the appropriate TTB officer. The application shall list the formulas for adoption by (1)...

  4. 7 CFR 1794.71 - Adoption of an EA.

    Science.gov (United States)

    2010-01-01

    ... 7 Agriculture 12 2010-01-01 2010-01-01 false Adoption of an EA. 1794.71 Section 1794.71... AGRICULTURE (CONTINUED) ENVIRONMENTAL POLICIES AND PROCEDURES Adoption of Environmental Documents § 1794.71 Adoption of an EA. RUS may adopt a Federal EA or EIS or a portion thereof as its EA. RUS shall make the...

  5. The Economics of Adoption of Children from Foster Care

    Science.gov (United States)

    Hansen, Mary Eschelbach; Hansen, Bradley A.

    2006-01-01

    Federal initiatives since 1996 have intensified the efforts of states to achieve adoption for children in foster care. For many waiting children, the path to adoption is long. The authors offer an economic analysis of adoption from foster care, with an emphasis on the reasons why achieving the goal of adoption for all waiting children may be so…

  6. Connecting the Dots: Exploring Themes in Adoption Picturebooks

    Science.gov (United States)

    Mattix, April A.; Crawford, Patricia A.

    2011-01-01

    Approximately 1.4 million school-aged children entered their families through adoption. As such, adoption represents a real and complex social issue with which young children--adopted or not--can grapple. Adoption picturebooks provide a medium by which children can explore this complicated concept. This manuscript analyzes a standard library…

  7. Using the Innovation Adoption Diffusion Model to Target Educational Programming.

    Science.gov (United States)

    Rollins, Timothy

    1993-01-01

    A telephone survey of 200 farm operators regarding adoption of innovations found 52% were early adopters or early majority adopters and 48% were late majority adopters or laggard. Importance of scientific research, learning about new concepts, and use of sources other than extension were factors distinguishing the categories. (SK)

  8. Understanding Technology Adoption: Theory and Future Directions for Informal Learning

    Science.gov (United States)

    Straub, Evan T.

    2009-01-01

    How and why individuals adopt innovations has motivated a great deal of research. This article examines individuals' computing adoption processes through the lenses of three adoption theories: Rogers's innovation diffusion theory, the Concerns-Based Adoption Model, the Technology Acceptance Model, and the United Theory of Acceptance and Use of…

  9. Adoption and the Guatemalan Journey to American Parenthood

    Science.gov (United States)

    Noonan, Emily J.

    2007-01-01

    Examining the adoption of Guatemalan children by US citizens, this article argues that adoptive parents make meanings and form identities through their participation in the adoption process and in their production of both Internet-based and spoken narratives about adoption. Using theories of globalization and narrative theory, the article…

  10. Partitioning the Adoption Process to Better Predict Permanency

    Science.gov (United States)

    McDonald, Tom; Press, Alan; Billings, Peggy; Moore, Terry

    2007-01-01

    Under federal outcome standards established by the Adoption and Safe Families Act of 1997, discharges to adoption are expected to occur within 24 months of the most recent removal from home for at least 32% of cases. In the research recounted here, adoption is treated as a process composed of two discrete steps: adoptive placement, and adoption…

  11. Enabling international adoption of LOINC through translation

    Science.gov (United States)

    Vreeman, Daniel J.; Chiaravalloti, Maria Teresa; Hook, John; McDonald, Clement J.

    2012-01-01

    Interoperable health information exchange depends on adoption of terminology standards, but international use of such standards can be challenging because of language differences between local concept names and the standard terminology. To address this important barrier, we describe the evolution of an efficient process for constructing translations of LOINC terms names, the foreign language functions in RELMA, and the current state of translations in LOINC. We also present the development of the Italian translation to illustrate how translation is enabling adoption in international contexts. We built a tool that finds the unique list of LOINC Parts that make up a given set of LOINC terms. This list enables translation of smaller pieces like the core component “hepatitis c virus” separately from all the suffixes that could appear with it, such “Ab.IgG”, “DNA”, and “RNA”. We built another tool that generates a translation of a full LOINC name from all of these atomic pieces. As of version 2.36 (June 2011), LOINC terms have been translated into 9 languages from 15 linguistic variants other than its native English. The five largest linguistic variants have all used the Part-based translation mechanism. However, even with efficient tools and processes, translation of standard terminology is a complex undertaking. Two of the prominent linguistic challenges that translators have faced include: the approach to handling acronyms and abbreviations, and the differences in linguistic syntax (e.g. word order) between languages. LOINC’s open and customizable approach has enabled many different groups to create translations that met their needs and matched their resources. Distributing the standard and its many language translations at no cost worldwide accelerates LOINC adoption globally, and is an important enabler of interoperable health information exchange PMID:22285984

  12. Adoptive parenting and attachment: association of the Internal Working Models between adoptive mothers and their late-adopted children during adolescence

    Directory of Open Access Journals (Sweden)

    Cecilia Serena ePace

    2015-09-01

    Full Text Available Introduction. Recent literature has shown that the good outcome of adoption would mostly depend on the quality of adoptive parenting, which is strongly associated with the security of parental internal working models (IWMs of attachment. Specifically, attachment states-of-mind of adoptive mothers classified as free and autonomous and without lack of resolution of loss or trauma could represent a good protective factor for adopted children previously maltreated and neglected. While most research on adoptive families has focused on pre-school and school-aged children, the aim of this study was to assess the concordance of IWMs of attachment in adoptive dyads during adolescence.Method. Our pilot-study involved 76 participants: 30 adoptive mothers (mean age=51.5±4.3, and their 46 late-adopted adolescents (mean age = 13.9 ± 1.6, who were all aged four to nine years old at time of adoption (M = 6.3 ± 1.5. Attachment representations of adopted adolescents were assessed by the Friend and Family Interview (FFI, while adoptive mothers’ state-of-mind with respect to attachment was classified by the Adult Attachment Interview (AAI. Adolescents’ verbal intelligence was controlled.Results. Late-adopted adolescents were classified as follows: 67% secure, 26% dismissing and 7% preoccupied in the FFI, while their adoptive mothers’ AAI classifications were 70% free-autonomous, 7% dismissing, and 23% unresolved. We found a significant concordance of 70% (32 dyads between the secure-insecure FFI and AAI classifications. Specifically adoptive mothers with high coherence of transcript and low unresolved loss tend to have late-adopted children with high secure attachment, even if the adolescents’ verbal intelligence made a significant contribution to this prediction.Discussion. Our results provides an empirical contribution to the literature concerning the concordance of attachment in adoptive dyads, highlighting the beneficial impact of highly coherent

  13. Adoptive parenting and attachment: association of the internal working models between adoptive mothers and their late-adopted children during adolescence

    OpenAIRE

    Pace, Cecilia S.; Di Folco, Simona; Guerriero, Viviana; Santona, Alessandra; Terrone, Grazia

    2015-01-01

    Introduction: Recent literature has shown that the good outcome of adoption would mostly depend on the quality of adoptive parenting, which is strongly associated with the security of parental internal working models (IWMs) of attachment. Specifically, attachment states-of-mind of adoptive mothers classified as free and autonomous and without lack of resolution of loss or trauma could represent a good protective factor for adopted children, previously maltreated and neglected. While most rese...

  14. New Innovation Adoption : Case Digi Electronics Ltd.

    OpenAIRE

    Eskelinen, Lauri

    2010-01-01

    The thesis was conducted for Digi Electronics Ltd which is web-based company in Hong Kong with selling consumer electronics to Finland as its main business. Currently, it is planning a totally new business branch – a virtual shop for tailor-made clothing. The research was to determine whether the target consumers would be ready to adopt the new innovation and also to identify the challenges faced by the business idea. The research process was started with a brainstorming session with the ...

  15. Customer Adoption of Internet Banking in Mauritius

    Directory of Open Access Journals (Sweden)

    N. Mamode Khan

    2011-05-01

    Full Text Available Internet banking offers many benefits but little research has been done about its acceptance in Mauritius. This paper aims at assessing the factors that contribute to the adoption of internet banking in Mauritius. To support our arguments, we use a logistic regression model based on a sample survey to analyze the factors that influence internet banking in Mauritius. We conclude that factors such as age, income, service usefulness, risk factor, checking account frequency and internet location are the main determinants for a person to opt for online banking

  16. Customer Adoption of Internet Banking in Mauritius

    Directory of Open Access Journals (Sweden)

    N. Mamode Khan

    2011-05-01

    Full Text Available Internet banking offers many benefits but little research has been done about its acceptance inMauritius. This paper aims at assessing the factors that contribute to the adoption of internetbanking in Mauritius. To support our arguments, we use a logistic regression model based on asample survey to analyze the factors that influence internet banking in Mauritius. We concludethat factors such as age, income, service usefulness, risk factor, checking account frequency andinternet location are the main determinants for a person to opt for online banking.

  17. Managers’ Identification with and Adoption of Telehealthcare

    Directory of Open Access Journals (Sweden)

    Jane Hendy

    2014-08-01

    Full Text Available This paper presents managerial attempts at implementing telehealthcare. Our longitudinal, ethnographic case studies document both successful and failed implementations across five health and social care organisations in England. We draw on theories of organisational identity, sensemaking and sensegiving to highlight how managerial organisational identities can inhibit the uptake of digital health technologies. Managers who strongly identified with their current role at work felt threatened by the intended change; a telehealthcare mode of care delivery. When a strongly identified workforce agrees with this assessment, managerial and employee sensemaking and sensegiving coalesce, forming a united front of resistance that prevents further adoption of the innovation.

  18. Adoption: biological and social processes linked to adaptation.

    Science.gov (United States)

    Grotevant, Harold D; McDermott, Jennifer M

    2014-01-01

    Children join adoptive families through domestic adoption from the public child welfare system, infant adoption through private agencies, and international adoption. Each pathway presents distinctive developmental opportunities and challenges. Adopted children are at higher risk than the general population for problems with adaptation, especially externalizing, internalizing, and attention problems. This review moves beyond the field's emphasis on adoptee-nonadoptee differences to highlight biological and social processes that affect adaptation of adoptees across time. The experience of stress, whether prenatal, postnatal/preadoption, or during the adoption transition, can have significant impacts on the developing neuroendocrine system. These effects can contribute to problems with physical growth, brain development, and sleep, activating cascading effects on social, emotional, and cognitive development. Family processes involving contact between adoptive and birth family members, co-parenting in gay and lesbian adoptive families, and racial socialization in transracially adoptive families affect social development of adopted children into adulthood. PMID:24016275

  19. What drives Web 2.0 adoption in South African civil society organisations

    Directory of Open Access Journals (Sweden)

    Kiru Pillay

    2014-10-01

    Full Text Available Background: The impact and consequences of social media adoption on society are only just being realised and studied in detail; consequently, there is no universal agreement as to the reasons for the adoption of these services. Even understanding why some social media services are popular remains to some extent elusive. The practical use of Web 2.0 does not provide any answers either with, for example, a noticeable difference in the way social media was strategically used by Barack Obama and Mitch Romney in the lead-up to the 2009 American elections. However, recent studies that have focused on social media adoption within specific sectors have begun to shed some light on these emerging adoption patterns; two studies in particular are illustrative: a 2012 study on the newspaper sector and a study on social media adoption and e-government. Objectives: This study investigates why South African civil society organisations (CSOs adopt Web 2.0 services and the perceived and actual benefits of such adoption. Method: A survey questionnaire was sent to 1712 South African CSOs listed in the Prodder database to explore why certain social media services were adopted and the perceived benefits thereof. Results: Internal reasons for the adoption of social media services by South African CSOs coalesce around organisational visibility and access to information. External reasons focus on organisations needing to become more relevant and more connected to like-minded organisations and initiatives. Conclusion: The pervasiveness of Web 2.0 technologies makes it inevitable that CSOs will have to restructure themselves to remain relevant.

  20. Pattern recognition

    CERN Document Server

    Theodoridis, Sergios

    2003-01-01

    Pattern recognition is a scientific discipline that is becoming increasingly important in the age of automation and information handling and retrieval. Patter Recognition, 2e covers the entire spectrum of pattern recognition applications, from image analysis to speech recognition and communications. This book presents cutting-edge material on neural networks, - a set of linked microprocessors that can form associations and uses pattern recognition to ""learn"" -and enhances student motivation by approaching pattern recognition from the designer's point of view. A direct result of more than 10

  1. Timing of Technology Adoption and Product Market Competition

    OpenAIRE

    Milliou, Chrysovalantou; Petrakis, Emmanuel

    2009-01-01

    This paper examines how product market competition affects firms' timing of adopting a new technology as well as whether the market provides sufficient adoption incentives. It shows that adoption dates differ not only among symmetric firms but also among markets with Cournot and Bertrand competition. More specifically, Cournot competition can lead to earlier adoption than Bertrand competition. It shows also that competition toughness does not always reinforce adoption incentives. When goods a...

  2. The role of information in technology adoption under poverty

    OpenAIRE

    Zhao, Jinhua

    2005-01-01

    An important channel through which globalization affects poverty is introducing new technologies to developing countries. Adoption of new technologies can be hindered by uncertainties about their efficiency. This paper studies the role of information exchange between adopters and others about a new technology and about each other’s likelihood of adoption. We show that information about the technology can reduce adoption at the beginning of the diffusion process and increase adoption later. In...

  3. Using Private Contracts to Create Adoptions from Foster Care

    OpenAIRE

    Mary Eschelbach Hansen

    2007-01-01

    Creating adoptions for children waiting in foster care is a good investment, but the number of adoptions created each year meets only a fraction of the need. This paper explores how the organization of the delivery of social services to waiting children and prospective adoptive families influences adoption creation. Cross-section time-series estimates are supplemented with a new augmented fixed effects procedure to demonstrate that the use of contracts with private agencies bolsters adoption ...

  4. Child adoption matching: preferences for gender and race

    OpenAIRE

    Baccara, Mariagiovanna; Collard-Wexler, Allan; Felli, Leonardo; Yariv, Leeat

    2013-01-01

    This paper uses a new data set on child-adoption matching to estimate the preferences of potential adoptive parents over U.S.-born and unborn children relinquished for adoption. We identify significant preferences favoring girls and unborn children close to birth, and against African-American children put up for adoption. These attitudes vary in magnitudes across different adoptive parents - heterosexual, same-sex couples, and single women. We also consider the effects of excluding single wom...

  5. COMPUTER AND INTERNET ADOPTION ON LARGE U.S. FARMS

    OpenAIRE

    Gloy, Brent A.; Akridge, Jay T.

    2000-01-01

    The adoption of the personal computer and the Internet is studied within a sample of large U.S. farms. Factors such as age and education influence the adoption of both technologies. Likewise, strong relationships exist between the adoption of each technology and the sophistication of farm management and the complexity of the farm business. After controlling for computer adoption, the results suggest there remain several factors limiting Internet adoption. It appears that producers are unsure ...

  6. Overcoming the Adoption Barrier to Electric Flight

    Science.gov (United States)

    Borer, Nicholas K.; Nickol, Craig L.; Jones, Frank P.; Yasky, Richard J.; Woodham, Kurt; Fell, Jared S.; Litherland, Brandon L.; Loyselle, Patricia L.; Provenza, Andrew J.; Kohlman, Lee W.; Samuel, Aamod G.

    2016-01-01

    Electrically-powered aircraft can enable dramatic increases in efficiency and reliability, reduced emissions, and reduced noise as compared to today's combustion-powered aircraft. This paper describes a novel flight demonstration concept that will enable the benefits of electric propulsion, while keeping the extraordinary convenience and utility of common fuels available at today's airports. A critical gap in airborne electric propulsion research is addressed by accommodating adoption at the integrated aircraft-airport systems level, using a confluence of innovative but proven concepts and technologies in power generation and electricity storage that need to reside only on the airframe. Technical discriminators of this demonstrator concept include (1) a novel, high-efficiency power system that utilizes advanced solid oxide fuel cells originally developed for ultra-long-endurance aircraft, coupled with (2) a high-efficiency, high-power electric propulsion system selected from mature products to reduce technical risk, assembled into (3) a modern, high-performance demonstration platform to provide useful and compelling data, both for the targeted early adopters and the eventual commercial market.

  7. Skin diseases in internationally adopted children.

    Science.gov (United States)

    Rigal, Émilie; Nourrisson, Céline; Sciauvaud, Julie; Pascal, Julie; Texier, Charlotte; Corbin, Violaine; Poirier, Véronique; Beytout, Jean; Labbe, André; Lesens, Olivier

    2016-08-01

    Internationally adopted children often present diseases contracted in the country of origin. Skin diseases are common in new arrivals, and diagnosis may prove challenging for GPs or even dermatologists if they are inexperienced in the extensive geographic and ethnic diversity of international adoptees. To analyse the frequency and characteristics of skin diseases in international adoptees. In total, 142 adoptees were evaluated for a cross-sectional cohort study. The most frequent diseases observed at arrival were dermatological conditions. Of the adoptees, 70% presented at least one skin disease, of which 57.5% were infectious; Tinea capitis being the most frequent (n = 42). The recovery rate of Tinea capitis was 89% (n = 32/36). Ten cases of scabies were diagnosed. Other diseases included viral skin infection (n = 22), with 16 cases of Molluscum contagiosum and bacterial infection. Skin diseases are very common in internationally adopted children. There is a need for close collaboration between dermatologists and paediatricians to diagnose such infections, as well as clear guidelines to treat them. PMID:27436771

  8. Endogenous Technology Adoption and Medical Costs.

    Science.gov (United States)

    Lamiraud, Karine; Lhuillery, Stephane

    2016-09-01

    Despite the claim that technology has been one of the most important drivers of healthcare spending growth over the past decades, technology variables are rarely introduced explicitly in cost equations. Furthermore, technology is often considered exogenous. Using 1996-2007 panel data on Swiss geographical areas, we assessed the impact of technology availability on per capita healthcare spending covered by basic health insurance whilst controlling for the endogeneity of health technology availability variables. Our results suggest that medical research, patent intensity and the density of employees working in the medical device industry are influential factors for the adoption of technology and can be used as instruments for technology availability variables in the cost equation. These results are similar to previous findings: CT and PET scanner adoption is associated with increased healthcare spending, whilst increased availability of percutaneous transluminal coronary angioplasty facilities is associated with reductions in per capita spending. However, our results suggest that the magnitude of these relationships is much greater in absolute value than that suggested by previous studies that did not control for the possible endogeneity of the availability of technologies. Copyright © 2016 John Wiley & Sons, Ltd.

  9. Adopting the Euro: Costs or Benefits?

    Directory of Open Access Journals (Sweden)

    Cristian Socol

    2007-01-01

    Full Text Available This paper evaluates the present estate of euro implementation in Monetary and Economic Union (EMU. The Romanian admittance in EU at January 2007 increases interest to study the transmission and coordination mechanisms, related to mix common monetary policy - different fiscal policy across the countries in the euro area. Our study focuses on Euro Zone using the Optimum Currency Area Theory and empirical evidences. The controversies beetwen economists regarding the construction process of EMU remain large. The paper evaluates the costs and benefits of adopting the euro and the main effects of Romanian integration in European Monetary Union. What are the consequences of implementing the "tye hands" strategy for Romanian financial authorities? Can Romania solve the potential conflict between real and nominal convergence relating the European integration? What is the best strategy for Romania with the aim to obtain a faster real convergence with the European Union countries? Can Romania maximize the benefits to adopting euro in 2014? Is the European Union a Optimum Currency Area?

  10. Information architecture: Profile of adopted standards

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-09-01

    The Department of Energy (DOE), like other Federal agencies, is under increasing pressure to use information technology to improve efficiency in mission accomplishment as well as delivery of services to the public. Because users and systems have become interdependent, DOE has enterprise wide needs for common application architectures, communication networks, databases, security, and management capabilities. Users need open systems that provide interoperability of products and portability of people, data, and applications that are distributed throughout heterogeneous computing environments. The level of interoperability necessary requires the adoption of DOE wide standards, protocols, and best practices. The Department has developed an information architecture and a related standards adoption and retirement process to assist users in developing strategies and plans for acquiring information technology products and services based upon open systems standards that support application software interoperability, portability, and scalability. This set of Departmental Information Architecture standards represents guidance for achieving higher degrees of interoperability within the greater DOE community, business partners, and stakeholders. While these standards are not mandatory, particular and due consideration of their applications in contractual matters and use in technology implementations Department wide are goals of the Chief Information Officer.

  11. Endogenous Technology Adoption and Medical Costs.

    Science.gov (United States)

    Lamiraud, Karine; Lhuillery, Stephane

    2016-09-01

    Despite the claim that technology has been one of the most important drivers of healthcare spending growth over the past decades, technology variables are rarely introduced explicitly in cost equations. Furthermore, technology is often considered exogenous. Using 1996-2007 panel data on Swiss geographical areas, we assessed the impact of technology availability on per capita healthcare spending covered by basic health insurance whilst controlling for the endogeneity of health technology availability variables. Our results suggest that medical research, patent intensity and the density of employees working in the medical device industry are influential factors for the adoption of technology and can be used as instruments for technology availability variables in the cost equation. These results are similar to previous findings: CT and PET scanner adoption is associated with increased healthcare spending, whilst increased availability of percutaneous transluminal coronary angioplasty facilities is associated with reductions in per capita spending. However, our results suggest that the magnitude of these relationships is much greater in absolute value than that suggested by previous studies that did not control for the possible endogeneity of the availability of technologies. Copyright © 2016 John Wiley & Sons, Ltd. PMID:27492052

  12. CRM ADOPTION IN A HIGHER EDUCATION INSTITUTION

    Directory of Open Access Journals (Sweden)

    Guy-Emmanuel Rigo

    2016-04-01

    Full Text Available More and more organisations, from private to public sectors, are pursuing higher levels of customer satisfaction, loyalty and retention. With this intent, higher education institutions (HEI have adopted CRM – Customer Relationship Management. In order to analyse some of the interesting aspects of this phenomenon n, we conducted an action research in a European Institute. The main research question we answered is “how to adopt a CRM strategy in a Higher Education Institution?” Some of the main findings of this study are (1 even though HEI’s main customer is the student, there are others stakeholders that a CRM project must consider; (2 universities can use their internal resources to implement a CRM project successfully; and (3 using Agile software methodology is an effective way to define clearer, more objective and more assertive technical requirements which result in a CRM software that meet send user’s expectations and organizational strategic goals. These findings can help other HEIs

  13. Adoption of an official ISEA glossary.

    Science.gov (United States)

    Zartarian, Valerie; Bahadori, Tina; McKone, Tom

    2005-01-01

    The International Society for Exposure Analysis (ISEA) and its Nomenclature Committee have been involved since the mid-1990s in an intermittent but ongoing effort to develop an official ISEA glossary. Several related activities have stimulated greater interest and discussion nationally and internationally on a common exposure language. Among these activities are a 1997 Journal of Exposure Analysis and Environmental Epidemiology feature article on exposure and dose definitions and a 1999-initiated project of the International Programme on Chemical Safety (IPCS) (WHO/ILO/UNEP) to confront terminology issues hindering harmonization in the area of exposure assessment. Recently, the ISEA members voted in support of adopting the IPCS glossary as the official ISEA glossary, and the ISEA Executive Board agreed to accept this recommendation. In this feature article, we (1) describe the process through which the ISEA adopted the IPCS glossary as the official ISEA glossary, (2) present the joint IPCS/ISEA glossary of terms and their definitions, and (3) discuss plans for how the glossary can be used by ISEA and updated over time by ISEA and IPCS. The glossary is intended to be a living document that reflects the latest usage and maintains international harmonization of exposure terminology that can be practically applied to improve communication in exposure and related fields. PMID:15562291

  14. Development and Validation of the "Attitudes toward Adoption Scale" and the "Gay and Lesbian Parents' Adoption Scale."

    Science.gov (United States)

    Shelley-Sireci, Lynn M.; Ciano-Boyce, Claudia

    Two studies were conducted to evaluate newly developed measures of attitudes about adoption. The Attitudes toward Adoption Scale was developed, based on research literature, to investigate the attitudes of people with and without experience with adoption toward adoption. Eighty-six college students completed this scale and made open-ended comments…

  15. Renewable energy adoption in an ageing population: Heterogeneity in preferences for micro-generation technology adoption

    International Nuclear Information System (INIS)

    Many countries are endeavouring to supply more of their energy from renewable resources. Such countries are also experiencing an aging population with a greater proportion of people aged ≥65 years. This demographic shift may reduce the uptake of renewable energy, if older person households are less inclined to accept change and adopt new technologies. This paper assesses whether such households have different behavioural responses to energy efficiency compared to the rest of society and investigates whether micro-generation renewable energy technologies are less likely to be adopted by these households. It uses conditional logit and mixed logit models to investigate the impact of age of household on primary heating adoption, and also to assess the impact of older households on the installation of discretionary micro-generation technologies (solar thermal, solar voltaic, and wind power) to supplement existing heating and lighting systems. Results indicate that primary heating choice is not affected but that older person households are less inclined to adopt micro-generation technologies. - Highlights: → Heterogeneity exists in decisions on micro-generation technology installation. → Older person households are less inclined to adopt micro-generation technologies. → Micro-generation technologies fail a social cost-benefit analysis test.

  16. Renewable energy adoption in an ageing population: Heterogeneity in preferences for micro-generation technology adoption

    Energy Technology Data Exchange (ETDEWEB)

    Willis, Ken, E-mail: Ken.Willis@ncl.ac.uk [School of Architecture, Planning and Landscape, University of Newcastle, Newcastle upon Tyne NE1 7RU (United Kingdom); Scarpa, Riccardo [Department of Economics, Waikato School of Management, University of Waikato, Hamilton (New Zealand); Gilroy, Rose; Hamza, Neveen [School of Architecture, Planning and Landscape, University of Newcastle, Newcastle upon Tyne NE1 7RU (United Kingdom)

    2011-10-15

    Many countries are endeavouring to supply more of their energy from renewable resources. Such countries are also experiencing an aging population with a greater proportion of people aged {>=}65 years. This demographic shift may reduce the uptake of renewable energy, if older person households are less inclined to accept change and adopt new technologies. This paper assesses whether such households have different behavioural responses to energy efficiency compared to the rest of society and investigates whether micro-generation renewable energy technologies are less likely to be adopted by these households. It uses conditional logit and mixed logit models to investigate the impact of age of household on primary heating adoption, and also to assess the impact of older households on the installation of discretionary micro-generation technologies (solar thermal, solar voltaic, and wind power) to supplement existing heating and lighting systems. Results indicate that primary heating choice is not affected but that older person households are less inclined to adopt micro-generation technologies. - Highlights: > Heterogeneity exists in decisions on micro-generation technology installation. > Older person households are less inclined to adopt micro-generation technologies. > Micro-generation technologies fail a social cost-benefit analysis test.

  17. Geodesic patterns

    KAUST Repository

    Pottmann, Helmut

    2010-07-26

    Geodesic curves in surfaces are not only minimizers of distance, but they are also the curves of zero geodesic (sideways) curvature. It turns out that this property makes patterns of geodesics the basic geometric entity when dealing with the cladding of a freeform surface with wooden panels which do not bend sideways. Likewise a geodesic is the favored shape of timber support elements in freeform architecture, for reasons of manufacturing and statics. Both problem areas are fundamental in freeform architecture, but so far only experimental solutions have been available. This paper provides a systematic treatment and shows how to design geodesic patterns in different ways: The evolution of geodesic curves is good for local studies and simple patterns; the level set formulation can deal with the global layout of multiple patterns of geodesics; finally geodesic vector fields allow us to interactively model geodesic patterns and perform surface segmentation into panelizable parts. © 2010 ACM.

  18. USING THE THEORY OF TECHNOLOGY ACCEPTANCE MODEL TO EXPLAIN TEENAGERS' ADOPTION OF SMARTPHONES IN TRANSYLVANIA

    OpenAIRE

    ALT MÓNIKA ANETTA; PÁL ZSUZSA; SEER LÁSZLÓ

    2011-01-01

    In Romania the mobile phones has been adopted rapidly, and the newer generation of these devices, the smartphones follow the same pattern. The main purpose of the research is to study the smartphone adoption process among teenagers from the 1st Macro-region in Romania. The originality of this paper is to study a very special and vulnerable market segment such as teenagers. In our study, we test a simplified Technology Acceptance Model on a convenience sample, which contains 1,864 questionnair...

  19. Ontology Design Patterns: Improving Findability and Composition

    OpenAIRE

    Hammar, Karl

    2014-01-01

    Ontology Design Patterns (ODPs) are intended to guide non-experts in performing ontology engineering tasks successfully. While being the topic of significant research efforts, the uptake of these ideas outside the academic community is limited. This paper summarises issues preventing broader adoption of Ontology Design Patterns among practitioners, with an emphasis on finding and composing such patterns, and presents early results of work aiming to overcome these issues.

  20. Home Energy Displays. Consumer Adoption and Response

    Energy Technology Data Exchange (ETDEWEB)

    LaMarche, Janelle [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Cheney, K. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Akers, C. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Roth, K. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Sachs, O. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States)

    2012-12-01

    The focus of this project was to investigate the factors influencing consumer adoption of Home Energy Displays (HEDs) and to evaluate electricity consumption in households with basic HEDs versus enhanced feedback methods - web portals or alerts. The team hypothesized that providing flexible and relatable information to users, in addition to a basic HED, would make feedback more effective and achieve persistent energy savings. In Phase I, Fraunhofer conducted three user research studies and found preferences for aesthetically pleasing, easy to understand feedback that is accessible through multiple media and offered free of charge. The deployment of HEDs in 150 households planned for Phase II encountered major recruitment and HED field deployment problems. In light of these challenges, the team is pursuing a modified study investigating the energy savings of a web portal versus alert-based energy feedback instead of a physical HED.

  1. The Adoption of Innovations in Library Organizations

    Directory of Open Access Journals (Sweden)

    Su-may Sheih Chen

    2001-12-01

    Full Text Available To enhance the quality of library and information service, libraries have borrowed from business theory and practice in planning and evaluating library services. However, an area of business theory that has received relatively little attention in library science is organizational innovation. Reports in the business literature provide evidence that innovation is essential for both development and survival. In increasingly unstable times of rapidly changing information technology, libraries must use the literature of innovation as well as that of planning and evaluation to shape better libraries. This paper explores key issues related to innovation in libraries including the nature, the barriers, and the ways to adopt and implement innovative strategies successfully. [Article content in Chinese

  2. From Norm Adoption to Norm Internalization

    Science.gov (United States)

    Conte, Rosaria; Andrighetto, Giulia; Villatoro, Daniel

    In this presentation, advances in modeling the mental dynamics of norms will be presented. In particular, the process from norm-adoption, possibly yielding new normative goals, to different forms of norm compliance will be focused upon, including norm internalization, which is at study in social-behavioral sciences and moral philosophy since long. Of late, the debate was revamped within the rationality approach pointing to the role of norm internalization as a less costly and more reliable enforcement system than social control. So far, poor attention was paid to the mental underpinnings of internalization. In this presentation, a rich cognitive model of different types, degrees and factors of internalization is shown. The initial implementation of this model on EMIL-A, a normative agent architecture developed and applied to the.

  3. Adopting Internet Standards for Orbital Use

    Science.gov (United States)

    Wood, Lloyd; Ivancic, William; da Silva Curiel, Alex; Jackson, Chris; Stewart, Dave; Shell, Dave; Hodgson, Dave

    2005-01-01

    After a year of testing and demonstrating a Cisco mobile access router intended for terrestrial use onboard the low-Earth-orbiting UK-DMC satellite as part of a larger merged ground/space IP-based internetwork, we reflect on and discuss the benefits and drawbacks of integration and standards reuse for small satellite missions. Benefits include ease of operation and the ability to leverage existing systems and infrastructure designed for general use, as well as reuse of existing, known, and well-understood security and operational models. Drawbacks include cases where integration work was needed to bridge the gaps in assumptions between different systems, and where performance considerations outweighed the benefits of reuse of pre-existing file transfer protocols. We find similarities with the terrestrial IP networks whose technologies we have adopted and also some significant differences in operational models and assumptions that must be considered.

  4. BUSINESS INTELLIGENCE ADOPTION IN LARGE ROMANIAN COMPANIES

    Directory of Open Access Journals (Sweden)

    Flavia CAIA

    2014-11-01

    Full Text Available The economic conditions and market competition create pressures on companies to adopt new technologies that can provide more efficient information and can support decision-making better. The purpose of the research is to investigate the decision support information systems in order to apprise and enhance the capacity of the entities to apply the new knowledge that BI produces for organizational success and competitiveness. The importance of the conducted research consists in identifying solutions to improve reporting and stimulate the entities to start using business intelligence (BI technologies, which facilitate obtaining new information, in order to ensure flexibility, resilience and provide answers to questions that go beyond what the pre-defined reports can do to support decision-making. The estimated result is a technical and operational overview of the large companies in Romania, drawing future directions for an improved competitive behaviour and strategic awareness, and identifying the significant factors for optimizing the decision-making process.

  5. Applying and Developing Patterns in Teaching

    DEFF Research Database (Denmark)

    Bennedsen, Jens; Eriksen, Ole

    2003-01-01

    A community of teachers and researchers within computer science has adopted the idea of patterns and developed a set of pedagogical patterns. These patterns capture best practices in teaching. From our research and teaching practice we have observed that pedagogical patterns are useful......, but there is a need for concepts and tools to justify and analyze the patterns. In every teaching community there exists a set of values characterizing what good teaching is about. Patterns may be measured by stating which values they imply and to what degree. Considering them as value-based patterns in teaching...... enriches the notion of pedagogical patterns. Inspired by conditions for learning we identify three values in teaching in the field of engineering-related educations. Further we present a value-based template for guidelines in teaching, causing a better understanding of the patterns and help teachers...

  6. Adoption of Mobile Banking Applications in Lebanon

    Directory of Open Access Journals (Sweden)

    Marc F Audi

    2016-04-01

    Full Text Available This research studied the impact of mobile banking adaptation in Lebanon. A brief overview of the Lebanese banking sector has shown how it is very dynamic and large. The overview also shows that the Lebanese banking sector is very competitive and changing with the development of new technologies and the availability of new services. To explain mobile banking applications in Lebanon two models were studied in the literature. The first is the innovation diffusion theory which has 5 different constructs: relative advantage, compatibility, communicability, trailability and complexity. The innovation diffusion theory talks about the path innovations take to reach final adopters. The technology acceptance model on the other hand relates the effects of it main components, perceived ease of use and perceived usefulness, on consumer attitude and intention to adopt. Our model is based on both theories and has 8 constructs. It shows the effect of the 5 constructs of the innovation diffusion theory merging perceived usefulness to relative advantage and replacing complexity with perceived ease of use. We have developed 8 hypotheses based on the construct of the model and to study the user-none user effect. A personal administered survey done using convenient sampling managed to gather 315 respondents from 5 different locations in Beirut after administering pilot testing. All variables were tested for validity and reliability using different tests. A biverate linear regression analysis was used to test the hypotheses and establish the relations between the different variables. We also did an independent t-test to analyze the user none-user effects. It is concluded that this paper with a discussion of the tests and recommending the best way to market mobile banking applications and designing a good version of mobile banking that looks appealing to consumers.

  7. The Economics of Adoption of Children from Foster Care

    OpenAIRE

    Mary Eschelbach Hansen; Bradley A. Hansen

    2005-01-01

    Federal initiatives since 1996 have intensified the efforts of states to achieve adoption for children in foster care with the case goal of adoption. For many waiting children, the path to adoption is long. We offer an economic analysis of adoption from foster care, with an emphasis on the reasons why it may be so difficult to achieve the goal of adoption for all waiting children. We then estimate the determinants of adoptions from foster care across the states using data for fiscal years 199...

  8. CONFLICT OF LAWS & FAMILY LAW-Adoption(1)

    Institute of Scientific and Technical Information of China (English)

    YANG KE

    2008-01-01

    Adoption involves the process by which the existing par ents cease to be the legal parents of the child and the adopters are substituted for the parents as if the child was born to them. There are so many reasons for adoption,such as inability to conceive,parents not wanting a child,and inability to provide circumstances essential for the child's healthy development.However,as the number of children available for adoption in New Zealand decreases, an increas ing number of couples look to overseas countries to adopt. Therefore,the transnational adoptions are becoming common.

  9. Innovation adoption in Agriculture: Innovators, Early Adoptors and Laggards

    OpenAIRE

    Diederen, P.J.M.; Meijl, van, H.; Wolters, A.; Bijak, K.

    2003-01-01

    This paper analyses the choice of a farmer to be an innovator, an early adopter or a laggard (an adopter of mature technologies or a non-adopter) in the adoption of innovations that are available on the market. We estimate a nested logit model with data from a large sample of Dutch farmers. We find that structural characteristics (farm size, market position, solvency, age of the farmer) explain the difference in adoption behaviour between innovators and early adopters on the one hand and lagg...

  10. Gender and Racial Biases: Evidence from Child Adoption

    OpenAIRE

    Baccara, Mariagiovanna; Collard-Wexler, Allan; Felli, Leonardo; Yariv, Leeat

    2010-01-01

    This paper uses a new data set on domestic child adoption to document the preferences of potential adoptive parents over born and unborn babies relinquished for adoption by their birth mothers. We show that adoptive parents exhibit significant biases in favor of girls and against African-American babies. A non-African-American baby relinquished for adoption attracts the interest of potential adoptive parents with probability 11.5% if it is a girl and 7.9% if it is a boy. As for race, a non-Af...

  11. Search Patterns

    CERN Document Server

    Morville, Peter

    2010-01-01

    What people are saying about Search Patterns "Search Patterns is a delight to read -- very thoughtful and thought provoking. It's the most comprehensive survey of designing effective search experiences I've seen." --Irene Au, Director of User Experience, Google "I love this book! Thanks to Peter and Jeffery, I now know that search (yes, boring old yucky who cares search) is one of the coolest ways around of looking at the world." --Dan Roam, author, The Back of the Napkin (Portfolio Hardcover) "Search Patterns is a playful guide to the practical concerns of search interface design. It cont

  12. Adopting Energy Saving Technology: Inertia or Incentives?

    OpenAIRE

    Peter A. Groothuis; Tanga McDaniel Mohr

    2013-01-01

    In an effort to improve efficiency of electrical markets the U.S. government hopes to encourage changing household use patterns, such as dishwasher and clothes dryer use, to off-peak times. One strategy has been to subsidize the installation of smart meters. In addition the government has encouraged electrical energy conservation by providing incentives for energy saving technologies such as the purchase of energy star appliances or increased insulation in the home. Households have sometimes ...

  13. Technology Adoption of Medical Faculty in Teaching: Differentiating Factors in Adopter Categories

    Science.gov (United States)

    Zayim, Nese; Yildirim, Soner; Saka, Osman

    2006-01-01

    Despite large investments by higher education institutions in technology for faculty and student use, instructional technology is not being integrated into instruction in higher education institutions including medical education institutions. While the diffusion of instructional technologies has reached a saturation point among early adopters of…

  14. Adoption of a Non-Lecture Pedagogy in Chemical Engineering: Insights Gained from Observing an Adopter

    Science.gov (United States)

    Golter, P. B.; Thiessen, D. B.; Van Wie, B. J.; Brown, G. R.

    2012-01-01

    Promoting the adoption of an alternative pedagogy can be a difficult process. Many professors are not interested in significantly modifying how they teach their course. Those that are interested in pedagogical reform still have concerns that must be addressed before or during an implementation. This paper presents a case study following the…

  15. PET imaging of adoptive progenitor cell therapies.

    Energy Technology Data Exchange (ETDEWEB)

    Gelovani, Juri G.

    2008-05-13

    Objectives. The overall objective of this application is to develop novel technologies for non-invasive imaging of adoptive stem cell-based therapies with positron emission tomography (PET) that would be applicable to human patients. To achieve this objective, stem cells will be genetically labeled with a PET-reporter gene and repetitively imaged to assess their distribution, migration, differentiation, and persistence using a radiolabeled reporter probe. This new imaging technology will be tested in adoptive progenitor cell-based therapy models in animals, including: delivery pro-apoptotic genes to tumors, and T-cell reconstitution for immunostimulatory therapy during allogeneic bone marrow progenitor cell transplantation. Technical and Scientific Merits. Non-invasive whole body imaging would significantly aid in the development and clinical implementation of various adoptive progenitor cell-based therapies by providing the means for non-invasive monitoring of the fate of injected progenitor cells over a long period of observation. The proposed imaging approaches could help to address several questions related to stem cell migration and homing, their long-term viability, and their subsequent differentiation. The ability to image these processes non-invasively in 3D and repetitively over a long period of time is very important and will help the development and clinical application of various strategies to control and direct stem cell migration and differentiation. Approach to accomplish the work. Stem cells will be genetically with a reporter gene which will allow for repetitive non-invasive “tracking” of the migration and localization of genetically labeled stem cells and their progeny. This is a radically new approach that is being developed for future human applications and should allow for a long term (many years) repetitive imaging of the fate of tissues that develop from the transplanted stem cells. Why the approach is appropriate. The novel approach to

  16. PET imaging of adoptive progenitor cell therapies

    International Nuclear Information System (INIS)

    The overall objective of this application is to develop novel technologies for non-invasive imaging of adoptive stem cell-based therapies with positron emission tomography (PET) that would be applicable to human patients. To achieve this objective, stem cells will be genetically labeled with a PET-reporter gene and repetitively imaged to assess their distribution, migration, differentiation, and persistence using a radiolabeled reporter probe. This new imaging technology will be tested in adoptive progenitor cell-based therapy models in animals, including: delivery pro-apoptotic genes to tumors, and T-cell reconstitution for immunostimulatory therapy during allogeneic bone marrow progenitor cell transplantation. Technical and Scientific Merits. Non-invasive whole body imaging would significantly aid in the development and clinical implementation of various adoptive progenitor cell-based therapies by providing the means for non-invasive monitoring of the fate of injected progenitor cells over a long period of observation. The proposed imaging approaches could help to address several questions related to stem cell migration and homing, their long-term viability, and their subsequent differentiation. The ability to image these processes non-invasively in 3D and repetitively over a long period of time is very important and will help the development and clinical application of various strategies to control and direct stem cell migration and differentiation. Approach to accomplish the work. Stem cells will be genetically with a reporter gene which will allow for repetitive non-invasive 'tracking' of the migration and localization of genetically labeled stem cells and their progeny. This is a radically new approach that is being developed for future human applications and should allow for a long term (many years) repetitive imaging of the fate of tissues that develop from the transplanted stem cells. Why the approach is appropriate. The novel approach to stem cell imaging

  17. Can gay and lesbian parents promote healthy development in high-risk children adopted from foster care?

    Science.gov (United States)

    Lavner, Justin A; Waterman, Jill; Peplau, Letitia Anne

    2012-10-01

    Adoption is known to promote cognitive and emotional development in children from foster care, but policy debates remain regarding whether children adopted by gay and lesbian parents can achieve these positive outcomes. This study compared the cognitive development and behavior problems at 2, 12, and 24 months postplacement of 82 high-risk children adopted from foster care in heterosexual and gay or lesbian households. On average, children in both household types showed significant gains in cognitive development and maintained similar levels of behavior problems over time, despite gay and lesbian parents raising children with higher levels of biological and environmental risks prior to adoptive placement. Results demonstrated that high-risk children show similar patterns of development over time in heterosexual and gay and lesbian adoptive households. PMID:23039344

  18. Assessing multifunctional innovation adoption via an integrative model

    NARCIS (Netherlands)

    Sääksjärvi, M.; Samiee, S.

    2010-01-01

    This study proposes and tests an integrative model that incorporates the mental resources framework (MOA: motivation, opportunity, and ability) alongside traditional innovation adoption predictors for assessing the adoption of dual-functionality innovations (DFI), a special case of multifunctional i

  19. Investors’ Adoption of Internet Stock Trading: A Study

    Directory of Open Access Journals (Sweden)

    Arwinder Singh

    2010-04-01

    Full Text Available The purpose of this paper wa s to examine whether investors who adopted Internet stock trading perceived differently from those of non - adopters. The primary data based on 299 investors (149 adopters and 150 non - adopters were analyzed using advanced multivariate techniques like factor analysis and discriminant analysis besides percentages, means, and Z - test. Results indicated that a ttitude dimensions and demographic variabl es contributed significantly in classifying investors as adopters or non - adopters in Internet trading. As regards attitude dimensions, ‘ variety of financial products and safety’ contributed significantly in discriminating between adopters and non - adopters of Internet trading followed by the factor such as ‘ convenience and transparency’ . As far as the demographics were concerned, the mature/older, experienced, and businessmen investors were less likely to use Internet stock trading as compared to young, inex perienced, and non - businessmen investors

  20. Drivers of Adoption and Implementation of Internet-Based Marketing Channels

    DEFF Research Database (Denmark)

    Nielsen, Jørn Flohr; Mols, Niels Peter; Høst, Viggo

    2007-01-01

    , specialised investments, and management support. This is mainly the case in small firms, as the importance of the various drivers and the adoption pattern vary between size categories. Market-pull factors have relatively more explanatory power in medium-sized and large companies.......This chapter analyses factors influencing manufacturers= adoption and implementation of Internet-based marketing channels, using models based on marketing channel and organisational innovation theory. Survey data from 1163 Danish, Finnish, and Swedish manufacturers form the empirical basis...... for testing the models using LISREL analysis. The results stress that adoption of Internet-based marketing is influenced by willingness to cannibalize, management support, market pressure, and a firm=s knowledge of IT. Willingness to cannibalize mediates the effects of future market orientation, ownership...

  1. Impact of the economic recession on companion animal relinquishment, adoption, and euthanasia: a Chicago animal shelter's experience.

    Science.gov (United States)

    Weng, Hsin-Yi; Hart, Lynette A

    2012-01-01

    This study investigated how the current economic recession (since December 2007) has affected dog and cat relinquishment, adoption, and euthanasia at the Anti-Cruelty Society animal shelter in Chicago, Illinois. The study compared temporal patterns of the investigated statistics before (2000-2007) the start of the current recession with the patterns after the start of the recession (2008-2010). The results showed that once the guardianship (ownership) of a nonhuman animal had been established, the recession did not greatly affect the owner's decision on relinquishment-except for the relinquishment of senior dogs, which may be associated with increased costs of care. However, an unfavorable economic environment may have reduced adoption of animals. The consequences of a decline in adoptions might be reflected in an increase in the proportion or number of sheltered animals euthanized. This study demonstrated how monitoring changes in temporal patterns in these shelter statistics can help guide animal shelters to better prepare for the current recession.

  2. ICT adoption policy of Australian and Croatian SMEs

    OpenAIRE

    Hazbo Skoko; Branka Krivokapic-Skoko; Marinko Skare; Arnela Ceric

    2013-01-01

    Many SMEs are currently adopting information and communication technology (ICT) and services based on it. However, there is little systematic research into how they are doing this and what are the organisational and environmental factors associated with this adoption. In this article, the authors build the model of ICT adoption in Australian and Croatian SMEs, founded on premises that SMEs are the main economic developing factor in all modern economies and that the adoption and the use of ICT...

  3. Depression and anxiety among postpartum and adoptive mothers

    OpenAIRE

    Mott, Sarah L.; Schiller, Crystal Edler; Richards, Jenny Gringer; O’Hara, Michael W.; Stuart, Scott

    2011-01-01

    Similar to biological mothers during the postpartum period, women who adopt children experience increased stress and life changes that may put them at risk for developing depression and anxiety. The purpose of the current study was to compare levels of depression and anxiety symptoms between postpartum and adoptive women and, among adoptive women, to examine associations between specific stressors and depressive symptoms. Data from adoptive mothers (n=147), recruited from Holt International, ...

  4. The diffusion/adoption of innovation in the internal market

    OpenAIRE

    Jordi Suriñach; Corinne Autant-Bernard; Fabio Manca; Nadine Massard; Rosina Moreno

    2009-01-01

    The main aim of the present study is to analyze the drivers of innovation adoption by (i) developing proper measures able to proxy for innovation adoption and internal market regulations, (ii) identifying the channels through which innovation adoption takes place and (iii) assessing the main determinants of this adoption process within the internal market. An original model is derived from the theoretical literature on innovation diffusion. Results show that the impact of the transmission cha...

  5. Intensity of Precision Agriculture Technology Adoption by Cotton Producers

    OpenAIRE

    Paxton, Kenneth W.; Mishra, Ashok K.; Chintawar, Sachin; Roberts, Roland K.; Larson, James A.; English, Burton C.; Lambert, Dayton M.; Marra, Michele C.; Larkin, Sherry L.; Reeves, Jeanne M.; Martin, Steven W

    2011-01-01

    Many studies on the adoption of precision technologies have generally used logit models to explain the adoption behavior of individuals. This study investigates factors affecting the intensity of precision agriculture technologies adopted by cotton farmers. Particular attention is given to the role of spatial yield variability on the number of precision farming technologies adopted, using a count data estimation procedure and farm-level data. Results indicate that farmers with more within-fie...

  6. Adoption process of information technology (IT) innovations in organizations

    OpenAIRE

    Abdul Hameed, Mumtaz

    2012-01-01

    This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University. Several models have been developed for understanding and predicting innovation adoption in organizations and literature has identified several factors that impact the adoption and implementation of Information Technology (IT). This research examines the process of adoption of IT innovations in organizations. The study explores the processes involved in the adoption of IT and verifies the key ...

  7. Should health care managers adopt Theory Z?

    Science.gov (United States)

    Safranski, S R; Kwon, I W; Walker, W R; Unger, M

    1986-04-01

    Health care administrators should carefully consider the situations in which they apply management methods used in industry, since such methods may not be effective in motivating certain groups of hospital employees. Physicians, for example, may display little loyalty to the health care organization, even though as a group they exert significant influence on policies, standards, and administration. As a result, management styles such as Theory Z that focus on holistic concern, individual decision-making responsibility, and long-term employment guarantees may fail to interest them. Nurses also may be reluctant to commit themselves to an organization because of the high rate of turnover in their profession in recent years. Support staff, however, probably would be receptive to management techniques that offer security through long-term employment guarantees. Other factors necessary for the effective use of Theory Z industrial management techniques are a clear hierarchy with well-defined reporting relationships, moderately specialized career paths, and trust among employees that the organization's concern for their welfare is genuine. The key consideration, however, in applying any theory is that only those aspects which best serve the organization's needs should be adopted.

  8. LEARNING STYLES ADOPTED BY MEDICAL STUDENTS

    Directory of Open Access Journals (Sweden)

    Chinmay Shah

    2011-12-01

    Full Text Available Learning results in gain of knowledge, skills and attitudes. Some like to learn by seeing, some by hearing and some by demonstration. Learning style influences the retention of information and depth of comprehension. Understanding their preferred learning styles as visual, auditory, read-write or kinesthetic learners will help improve the teaching methods adopted. Role of the educator necessitates making the most of each teaching opportunity by understanding the characteristics of the learning audience and incorporating demonstrated principles of adult educational design, with a focus on collaborative learning and variety in presentation techniques. The goal is to provide student oriented education, producing efficient doctors. A cross-sectional study among 92 medical students of the Govt. Medical College, Bhavnagar, conducted in 2009. VARK questionnaire was used to access their learning preference. Preference for different learning styles were, visual (V 1.08%, auditory (A 20.65%, reading/writing (R 2.17% and kinesthetic (K 17.39%. 41.30% of the total 92 students preferred a single mode of information presentation. Of the 92 students who preferred multiple modes of information presentation, some preferred two modes (bimodal, 31.52%, some preferred three modes (tri-modal, 27.13%, and only one student preferred quadri-modal.

  9. Home Energy Displays: Consumer Adoption and Response

    Energy Technology Data Exchange (ETDEWEB)

    LaMarche, J.; Cheney, K.; Akers, C.; Roth, K.; Sachs, O.

    2012-12-01

    The focus of this project was to investigate the factors influencing consumer adoption of Home Energy Displays (HEDs) and to evaluate electricity consumption in households with basic HEDs versus enhanced feedback methods - web portals or alerts. We hypothesized that providing flexible and relatable information to users, in addition to a basic HED, would make feedback more effective and achieve persistent energy savings. In Phase I, we conducted three user research studies and found preferences for aesthetically pleasing, easy to understand feedback that is accessible through multiple media and offered free of charge. The deployment of HEDs in 150 households planned for Phase II encountered major recruitment and HED field deployment problems. First, after extensive outreach campaigns to apartment complexes with 760 units, only 8% of building's tenants elected to receive a free HED in their homes as part of the field study. Second, the HED used, a leading market model, had a spectrum of problems, including gateway miscommunications, failure to post to a data-hosting third party, and display malfunctions. In light of these challenges, we are pursuing a modified study investigating the energy savings of a web portal versus alert-based energy feedback instead of a physical HED.

  10. Adopting Intercultural Communication Issue in Teaching English

    Directory of Open Access Journals (Sweden)

    Hussain Ahmed Liton

    2016-05-01

    Full Text Available Cultural assimilation and intercultural awareness has been an important hub of modern language and communication studies, underlining a shift that reflects a greater significance of the inseparability of language and culture, and the need to prepare students for effective intercultural communication to thrive in a global work environment. A thriving global workplace requires effective communication skill across cultures in this era of globalization and mass migration. Under the tutelage of such backdrop, this article addresses the terrain of adopting intercultural awareness in EFL classroom teaching/learning and aims at linking culture with language in pursuit of excellence in borderless effective communication. The study, in other words, investigated the linguistic aspects that could be affected by certain cultural dimensions (e.g., beliefs, traditions, taboo words, habits, and norms, religion, social factors, etc. in intercultural communication. This paper uses a questionnaire device to receive some university teachers’ self-reported feedback. This article maintains qualitative and quantitative research methodology. The analytical research result shows that in teaching English, it is necessary to incorporate and develop aspects of cross-cultural awareness as a part of course curriculum to immerse students in effective intercultural communicative competence (ICC.

  11. 45 CFR 1356.41 - Nonrecurring expenses of adoption.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 4 2010-10-01 2010-10-01 false Nonrecurring expenses of adoption. 1356.41 Section..., FOSTER CARE MAINTENANCE PAYMENTS, ADOPTION ASSISTANCE, AND CHILD AND FAMILY SERVICES REQUIREMENTS APPLICABLE TO TITLE IV-E § 1356.41 Nonrecurring expenses of adoption. (a) The amount of the payment made...

  12. Adoption of Conservation-Tillage Methods and Genetically Modified Cotton

    OpenAIRE

    Roberts, Roland K.; English, Burton C.; Gao, Qi; Larson, James A.

    2006-01-01

    Adoption of herbicide-tolerant cotton and conservation tillage may be simultaneously related. Bayes' theorem and a two-equation logit model were used to test the simultaneity hypothesis. Evidence for Tennessee suggests that adoption of these technologies reduced residual herbicide use and soil erosion more than if adoption of these technologies were independent.

  13. 49 CFR 389.29 - Adoption of final rules.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 5 2010-10-01 2010-10-01 false Adoption of final rules. 389.29 Section 389.29 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL MOTOR CARRIER SAFETY...-FEDERAL MOTOR CARRIER SAFETY REGULATIONS Procedures for Adoption of Rules § 389.29 Adoption of final...

  14. 14 CFR 221.162 - Receiver shall file adoption notices.

    Science.gov (United States)

    2010-01-01

    ... 14 Aeronautics and Space 4 2010-01-01 2010-01-01 false Receiver shall file adoption notices. 221... (AVIATION PROCEEDINGS) ECONOMIC REGULATIONS TARIFFS Adoption Publications Required To Show Change in Carrier's Name or Transfer of Operating Control § 221.162 Receiver shall file adoption notices. A...

  15. 45 CFR 1151.43 - Adoption of grievance procedures.

    Science.gov (United States)

    2010-10-01

    ... 45 Public Welfare 3 2010-10-01 2010-10-01 false Adoption of grievance procedures. 1151.43 Section 1151.43 Public Welfare Regulations Relating to Public Welfare (Continued) NATIONAL FOUNDATION ON THE... Enforcement § 1151.43 Adoption of grievance procedures. A recipient may adopt an internal grievance...

  16. 49 CFR 553.29 - Adoption of final rules.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Adoption of final rules. 553.29 Section 553.29... ADMINISTRATION, DEPARTMENT OF TRANSPORTATION RULEMAKING PROCEDURES Procedures for Adoption of Rules § 553.29 Adoption of final rules. Final rules are prepared by representatives of the office concerned and the...

  17. 49 CFR 237.31 - Adoption of bridge management programs.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 4 2010-10-01 2010-10-01 false Adoption of bridge management programs. 237.31 Section 237.31 Transportation Other Regulations Relating to Transportation (Continued) FEDERAL RAILROAD... Adoption of bridge management programs. Each track owner shall adopt a bridge safety management program...

  18. 49 CFR 1110.8 - Adoption of final rules.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 8 2010-10-01 2010-10-01 false Adoption of final rules. 1110.8 Section 1110.8 Transportation Other Regulations Relating to Transportation (Continued) SURFACE TRANSPORTATION BOARD, DEPARTMENT... Adoption of final rules. If, after consideration of all comments received, final rules are adopted,...

  19. 18 CFR 401.24 - Preparation and adoption.

    Science.gov (United States)

    2010-04-01

    ... adoption. 401.24 Section 401.24 Conservation of Power and Water Resources DELAWARE RIVER BASIN COMMISSION ADMINISTRATIVE MANUAL RULES OF PRACTICE AND PROCEDURE Water Resources Program § 401.24 Preparation and adoption. The Water Resources Program will be prepared and considered by the Commission for adoption...

  20. 10 CFR 1021.103 - Adoption of CEQ NEPA regulations.

    Science.gov (United States)

    2010-01-01

    ... PROCEDURES General § 1021.103 Adoption of CEQ NEPA regulations. DOE adopts the regulations for implementing NEPA published by CEQ at 40 CFR parts 1500 through 1508. ... 10 Energy 4 2010-01-01 2010-01-01 false Adoption of CEQ NEPA regulations. 1021.103 Section...

  1. Factors leading to inflation targeting : The impact of adoption

    NARCIS (Netherlands)

    Samarina, Anna; Sturm, Jan-Egbert

    2013-01-01

    This paper examines how the analysis of inflation targeting (IT) adoption is affected by allowing for a structural change after adoption, using panel probit models for 60 countries over the period 1985-2008. Our findings suggest that there is a structural change after IT adoption. Including the post

  2. Development and Adjustment of Adopted Adolescents : Longitudinal and Concurrent Factors

    NARCIS (Netherlands)

    Jaffari-Bimmel, Nicole

    2005-01-01

    The first study of this thesis (Chapter 2) showed that the majority of the internationally adopted children are well adjusted, although a relatively large minority of adopted children had behavior problems of clinical significance or were referred to mental health services compared with non-adopted

  3. Counseling Adopted Persons in Adulthood: Integrating Practice and Research

    Science.gov (United States)

    Baden, Amanda L.; O'Leary Wiley, Mary

    2007-01-01

    For the past 50 years, adults who were adopted during infancy have been research participants for empirical studies with goals ranging from twin studies for heritability, to adjustment following adoption, to attachment. While the research body is broad, it has given little attention to counseling practices with adopted adults. Because empirical…

  4. Stepwise innovation adoption : a neglected concept in innovation research

    NARCIS (Netherlands)

    Huizingh, K.R.E.; Brand, M.J.

    2009-01-01

    Most innovation researchers tend to consider innovation adoption as a binary process, implying that companies have either adopted an innovation or not. In this paper we focus on e-commerce as an innovation that can be adopted stepwise. We distinguish between two levels of e-commerce, basic and advan

  5. Record-Keeping Technology Adoption among Dairy Farmers

    OpenAIRE

    Grisham, Elisabeth; Gillespie, Jeffrey

    2008-01-01

    Louisiana farmers were surveyed to determine their adoption of information and record-keeping technologies, including the Internet, Dairy Herd Improvement Association membership, use of financial measures, and frequency of use of computerized records. Factors influencing adoption included having a family successor, overall technology adoption propensity, diversification, off-farm income, college degree, and others.

  6. Record-Keeping Technology Adoption in the Louisiana Dairy Industry

    OpenAIRE

    Grisham, Elisabeth; Gillespie, Jeffrey M.

    2007-01-01

    Louisiana farmers were surveyed to determine their adoption of information and record-keeping technologies, including the internet, DHIA, use of financial measures, and frequency of use of computerized records. Factors influencing adoption included having a family successor, overall technology adoption propensity, diversification, off-farm income, college degree, and others.

  7. Toward a Process View in Adoption of Interorganizational Information Systems

    DEFF Research Database (Denmark)

    Brandt, Charlotte J.

    2014-01-01

    review found four different streams of research: 1) studies explaining why organizations adopt or reject IOIS by investigating factors determining adoption of or barriers to IOIS adoption; 2) studies explaining how to create the desired organizational effects from IOIS; 3) studies explaining how IOIS...

  8. Post-Adoption Depression: Clinical Windows on an Emerging Concept

    Science.gov (United States)

    Speilman, Eda

    2011-01-01

    In recent years, the concept of post-adoption depression--with both parallels and differences from postpartum depression--has emerged as a salient descriptor of the experience of a significant minority of newly adoptive parents. This article offers a clinical perspective on post-adoption depression through the stories of several families seen in…

  9. Optimal signal patterns for dynamical cellular communication

    CERN Document Server

    Hasegawa, Yoshihiko

    2015-01-01

    Cells transmit information via signaling pathways, using temporal dynamical patterns. As optimality with respect to environments is the universal principle in biological systems, organisms have acquired an optimal way of transmitting information. Here we obtain optimal dynamical signal patterns which can transmit information efficiently (low power) and reliably (high accuracy) using the optimal control theory. Adopting an activation-inactivation decoding network, we reproduced several dynamical patterns found in actual signals, such as steep, gradual and overshooting dynamics. Notably, when minimizing the power of the input signal, optimal signals exhibit the overshooting pattern, which is a biphasic pattern with transient and steady phases; this pattern is prevalent in actual dynamical patterns as it can be generated by an incoherent feed-forward loop (FFL), a common motif in biochemical networks. We also identified conditions when the three patterns, steep, gradual and overshooting, confer advantages.

  10. Adoption as a Social Marker: The Diffusion of Products in a Multigroup Environment

    CERN Document Server

    Smaldino, Paul E; Hillis, Vicken; Bednar, Jenna

    2015-01-01

    Social identities are among the key factors driving social behavior in complex societies. Recent attention to social identity in consumer behavior indicates that individuals avoid products that might signal membership in an outgroup. Yet the population-level consequences of adoption as identity signaling are largely unknown. Whereas previous work has focused on asymmetric attraction and repulsion among groups with different social identities, here we consider the spread of innovations in a structured population in which there are multiple groups who don't want to be mistaken for one another, using both analytical and agent-based modeling. This formal analysis, the first to take the spatial structure of a population into account, allows us to consider empirically important factors, including demographic skew and communication scale, that likely influence overall patterns of adoption. We find that as products become emergent social markers, aversion to outgroup-associated products can decrease overall patterns ...

  11. Pattern Formation

    Science.gov (United States)

    Hoyle, Rebecca

    2006-03-01

    From the stripes of a zebra and the spots on a leopard's back to the ripples on a sandy beach or desert dune, regular patterns arise everywhere in nature. The appearance and evolution of these phenomena has been a focus of recent research activity across several disciplines. This book provides an introduction to the range of mathematical theory and methods used to analyse and explain these often intricate and beautiful patterns. Bringing together several different approaches, from group theoretic methods to envelope equations and theory of patterns in large-aspect ratio-systems, the book also provides insight behind the selection of one pattern over another. Suitable as an upper-undergraduate textbook for mathematics students or as a fascinating, engaging, and fully illustrated resource for readers in physics and biology, Rebecca Hoyle's book, using a non-partisan approach, unifies a range of techniques used by active researchers in this growing field. Accessible description of the mathematical theory behind fascinating pattern formation in areas such as biology, physics and materials science Collects recent research for the first time in an upper level textbook Features a number of exercises - with solutions online - and worked examples

  12. Mapping factors influencing EAI adoption in the local government authorities on different phases of the adoption lifecycle

    OpenAIRE

    Kamal, M.; Themistocleous, M; Elliman, T

    2008-01-01

    Several private and public organisations have adopted Enterprise Application Integration (EAI), however, its application in the Local Government Authorities (LGAs) is limited. Although, there exist few EAI adoption models, these models mainly focus on a number of different factors (e.g. benefits, barriers, cost) influencing the decision making process for EAI adoption. Moreover, these models do not illustrate which factor(s) influence the decision making process for EAI adoption on the adopti...

  13. Internationally Adopted Children from Non-European Countries: General Development during the First Two Years in the Adoptive Family

    OpenAIRE

    Monica Dalen; Steinar Theie

    2012-01-01

    Internationally adopted children are often delayed in their development and demonstrate more behaviour problems than nonadopted children due to adverse preadoption circumstances. This is especially true for children adopted from Eastern European countries. Few studies have focused on children adopted from non-European countries. This paper presents results from an ongoing longitudinal study of 119 internationally adopted children from non-European countries during their first two years in Nor...

  14. Technology Adoption in Post-Conflict Regions: EDI Adoption in Kosovo After the War

    OpenAIRE

    Larry Stapleton

    2011-01-01

    Post-conflict developing regions are special cases of developing countries which have received little attention in information systems research. They are emergency situations which attract significant aid designed to help create economic stability through, for example, the use of IT. This study compared the experiences of Electronic Data Interchange (EDI) adoption in the extreme environment of a post-conflict region to other developing regions. Presenting data gathered from 68 companies in Ko...

  15. Adopting Open Innovation : A New Framework for the Analysis of the Open Innovation Adoption Process

    OpenAIRE

    Ferguson, Jennifer; Ukkonen, Merja

    2012-01-01

    Background: The term Open Innovation describes a phenomenon where organizations are no longer solely relying on internal R&D to innovate technologies that they then would bring to market. Instead, firms are looking outside for ideas or working with other organizations to develop technologies in order to capitalize on efficiencies of existing knowledge, reduce investment risks and increase speed to market. We identified a need to analyze the existing open innovation adoption frameworks in ...

  16. CUSTOMERS’ ADOPTION BEHAVIOUR IN INTERNET BANKING : A COMPARATIVE ANALYSIS OF GHANA AND SWEDEN

    OpenAIRE

    Acka, Germain Kofi; Ntsiful, Samuel; Odorh, Obinna

    2010-01-01

    Internet banking has now become a global phenomenon. It is the fastest growing service that banks can offer in order to gain and retain new customers. However, the issue of adoption behaviour or pattern by final consumers seems to be a pivotal issue in the successful implementation of Internet banking which has benefited people from jurisdiction to jurisdiction and from country to country. In some countries, Internet banking has proven to be very reliable, effective and efficient whilst in so...

  17. The Local Influence of Pioneer Investigators on Technology Adoption: Evidence from New Cancer Drugs

    OpenAIRE

    Leila Agha; David Molitor

    2015-01-01

    Local opinion leaders may play a key role in easing information frictions associated with technology adoption. This paper analyzes the influence of physician investigators who lead pivotal clinical trials for new cancer drugs. By comparing diffusion patterns across many drugs, we separate correlated regional demand for new technology from information spillovers. Using original data on clinical trial study authors for 21 new cancer drugs along with Medicare claims data from 1998-2008, we find ...

  18. The ART of life: IVF or child adoption?

    OpenAIRE

    Gumus, Gulcin; Lee, Jungmin

    2010-01-01

    This paper analyzes the effects of child adoption on the utilization of assisted reproductive technology (ART) in the US. Using state-level longitudinal data for 1999-2006, we show that ART use is responsive to changes in adoption markets. Controlling for state-specific fixed effects, the estimated elasticity of ART cycles performed with respect to child adoptions is about -0.13 to -0.15. The responsiveness is higher when we consider infant adoptions, adoptions by older women, and internation...

  19. Mediating Discourses of Transnational Adoption on the Internet

    DEFF Research Database (Denmark)

    McIlvenny, Paul; Raudaskoski, Pirkko Liisa

    2005-01-01

    Transnational (or intercountry) adoption is a global phenomenon with a diverse range of practices which are increasingly under scrutiny. Notable differences are found in practices across both ‘sending’ countries (such as China, Columbia, India, South Africa and South Korea, and ‘receiving......, the prospective adopter(s) is/are an intense site of inspection, an intersection of a broad range of medical, judicial, educational, psychological and linguistic practices. Increasingly, adopters have been using the Internet to prepare for and monitor the adoption process and to publish accounts...

  20. Adoption in rock and white-tailed ptarmigan

    Science.gov (United States)

    Wong, M.M.L.; Fedy, B.C.; Wilson, S.; Martin, K.M.

    2009-01-01

    Reports of adoption in birds are widespread, but few studies report rates of adoption or possible mechanisms for this phenomenon, particularly in the Order Galliformes. We report incidents of adoption in Rock Ptarmigan (Lagopus muta) and White-tailed Ptarmigan (L. leucura) from two sites in western Canada. Adoption rates for White-tailed Ptarmigan on Vancouver Island, British Columbia, and the Ruby Ranges, Yukon Territory were 13% (n = 16 broods) and 4% (n = 27), respectively, while rates for Rock Ptarmigan were 14% (n = 29) in the Ruby Ranges. Low brood densities may result in lower rates of adoption for ptarmigan. ?? 2009 The Wilson Ornithological Society.