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Sample records for allele-specific patterns adopted

  1. Structural allele-specific patterns adopted by epitopes in the MHC-I cleft and reconstruction of MHC:peptide complexes to cross-reactivity assessment.

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    Dinler A Antunes

    Full Text Available The immune system is engaged in a constant antigenic surveillance through the Major Histocompatibility Complex (MHC class I antigen presentation pathway. This is an efficient mechanism for detection of intracellular infections, especially viral ones. In this work we describe conformational patterns shared by epitopes presented by a given MHC allele and use these features to develop a docking approach that simulates the peptide loading into the MHC cleft. Our strategy, to construct in silico MHC:peptide complexes, was successfully tested by reproducing four different crystal structures of MHC-I molecules available at the Protein Data Bank (PDB. An in silico study of cross-reactivity potential was also performed between the wild-type complex HLA-A2-NS31073 and nine MHC:peptide complexes presenting alanine exchange peptides. This indicates that structural similarities among the complexes can give us important clues about cross reactivity. The approach used in this work allows the selection of epitopes with potential to induce cross-reactive immune responses, providing useful tools for studies in autoimmunity and to the development of more comprehensive vaccines.

  2. Allele specific expression in worker reproduction genes in the bumblebee Bombus terrestris

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    Harindra E. Amarasinghe

    2015-07-01

    Full Text Available Methylation has previously been associated with allele specific expression in ants. Recently, we found methylation is important in worker reproduction in the bumblebee Bombus terrestris. Here we searched for allele specific expression in twelve genes associated with worker reproduction in bees. We found allele specific expression in Ecdysone 20 monooxygenase and IMP-L2-like. Although we were unable to confirm a genetic or epigenetic cause for this allele specific expression, the expression patterns of the two genes match those predicted for imprinted genes.

  3. Allelic-specific expression in relation to Bombyx mori resistance to Bt toxin.

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    Chen, Yazhou; Li, Muwang; Islam, Iftakher; You, Lang; Wang, Yueqiang; Li, Zhiqian; Ling, Lin; Zeng, Baosheng; Xu, Jun; Huang, Yongping; Tan, Anjiang

    2014-11-01

    Understanding the mechanism of Bt resistance is one of the key elements of the effective application of Bt in pest control. The lepidopteran model insect, the silkworm, demonstrates qualities that make it an ideal species to use in achieving this understanding. We screened 45 strains of silkworm (Bombyx mori) using a Cry1Ab toxin variant. The sensitivity levels of the strains varied over a wide range. A resistant strain (P50) and a phylogenetically related susceptible strain (Dazao) were selected to profile the expressions of 12 Bt resistance-related genes. The SNPs in these genes were detected based on EST analysis and were validated by allelic-specific PCR. A comparison of allelic-specific expression between P50 and Dazao showed that the transcript levels of heterozygous genes containing two alleles rather than an imbalanced allelic expression contribute more to the resistance of P50 against Bt. The responses of the allelic-specific expression to Bt in hybrid larvae were then investigated. The results showed that the gene expression pattern of an ATP-binding cassette transporter C2 (ABCC2) and an aminopeptidase N (APN3), changed in an allelic-specific manner, with the increase of the resistant allele expression correlated with larval survival. The results suggest that a trans-regulatory mechanism in ABCC2 and APN3 allelic-specific expression is involved in the insect's response to the Bt toxin. The potential role of allelic-specific gene regulation in insect resistance to Bt toxins is discussed.

  4. Adopting Collaborative Workflow Pattern : Use Case

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    Antonio Capodieci

    2015-03-01

    Full Text Available In recent years, the use of web 2.0 tools has incre ased in companies and organisation. This phenomenon, has modified common organisational and operative practices. This has led “knowledge workers” to change their working practic es through the use of Web 2.0 communication tools. Unfortunately, these tools hav e not been integrated with existing enterprise information systems. This is an importan t problem in an organisational context because knowledge of information exchanged is neede d. In previous works we demonstrate that it is possible to capture this knowledge using coll aboration processes, which are processes of abstraction created in accordance with design patte rns and applied to new organisational operative practices. In this article, we want to pr esent the experience of the adoption of the methodology and the catalog of patterns from some p attern designers of a company operating in Public Administration and Finance, with the aim of shaping an information system Enterprise 2.0, which takes into account the collaborative pro cesses.

  5. Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus.

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    Fujita, Toshitsugu; Yuno, Miyuki; Fujii, Hodaka

    2016-07-28

    The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adopted for a wide range of biological applications including genome editing. In some cases, dissection of genome functions requires allele-specific genome editing, but the use of CRISPR for this purpose has not been studied in detail. In this study, using the p16INK4a gene in HCT116 as a model locus, we investigated whether chromatin states, such as CpG methylation, or a single-nucleotide gap form in a target site can be exploited for allele-specific locus binding and genome editing by CRISPR in vivo. First, we showed that allele-specific locus binding and genome editing could be achieved by targeting allele-specific CpG-methylated regions, which was successful for one, but not all guide RNAs. In this regard, molecular basis underlying the success remains elusive at this stage. Next, we demonstrated that an allele-specific single-nucleotide gap form could be employed for allele-specific locus binding and genome editing by CRISPR, although it was important to avoid CRISPR tolerance of a single nucleotide mismatch brought about by mismatched base skipping. Our results provide information that might be useful for applications of CRISPR in studies of allele-specific functions in the genomes.

  6. Understanding Community Policing as an Innovation: Patterns of Adoption

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    Morabito, Melissa Schaefer

    2010-01-01

    In the 1980s and 1990s, community policing was viewed by many as a radical innovation in the field of policing, with the vast majority of police agencies reporting to have adopted the approach. Despite its overwhelming popularity, most police agencies did not adopt the central elements of community policing. This study examines patterns of…

  7. Allele-Specific DNA Methylation Detection by Pyrosequencing®

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    Sommer Kristensen, Lasse; Johansen, Jens Vilstrup; Grønbæk, Kirsten

    2015-01-01

    DNA methylation is an epigenetic modification that plays important roles in healthy as well as diseased cells, by influencing the transcription of genes. In spite the fact that human somatic cells are diploid, most of the currently available methods for the study of DNA methylation do not provide......-effective protocol for allele-specific DNA methylation detection based on Pyrosequencing(®) of methylation-specific PCR (MSP) products including a single nucleotide polymorphism (SNP) within the amplicon....

  8. RNA-FISH to analyze allele-specific expression.

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    Braidotti, G

    2001-01-01

    One of the difficulties associated with the analysis of imprinted gene expression is the need to distinguish RNA synthesis occurring at the maternal vs the paternally inherited copy of the gene. Most of the techniques used to examine allele-specific expression exploit naturally occurring polymorphisms and measure steady-state levels of RNA isolated from a pool of cells. Hence, a restriction fragment length polymorphism (RFLP) an be exploited in a heterozygote, by a reverse transcriptase polymerase chain reaction (RT-PCR)- based procedure, to analyze maternal vs paternal gene expression. The human IGF2R gene was analyzed in this way. Smrzka et al. (1) were thus able to show that the IGF2R gene possesses a hemimethylated, intronic CpG island analogous to the mouse imprinting box. However, IGF2R mRNA was detected that possessed the RFLP from both the maternal and paternal alleles in all but one of the 70 lymphoblastoid samples. (The one monoallelic sample reactivated its paternal allele with continued cell culturing.) It was concluded that monoallelic expression of the human gene is a polymorphic trait occurring in a small minority of all tested samples (reviewed in refs. 2,3). Although this is a sound conclusion, the question remains: Is the human IGF2R gene imprinted?

  9. Allele-specific deposition of macroH2A1 in Imprinting Control Regions

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    Choo, J H; Kim, J D; Chung, J H; Stubbs, L; Kim, J

    2006-01-13

    In the current study, we analyzed the deposition patterns of macroH2A1 at a number of different genomic loci located in X chromosome and autosomes. MacroH2A1 is preferentially deposited at methylated CpG CpG-rich regions located close to promoters. The macroH2A1 deposition patterns at the methylated CpG islands of several imprinted domains, including the Imprinting Control Regions (ICRs) of Xist, Peg3, H19/Igf2 Igf2, Gtl2/Dlk1, and Gnas domains, show consistent allele-specificity towards inactive, methylated alleles. The macroH2A1 deposition levels at the ICRs and other Differentially Methylated Regions (DMRs) of these domains are also either higher or comparable to those observed at the inactive X chromosome of female mammals. Overall, our results indicate that besides DNA methylation macroH2A1 is another epigenetic component in the chromatin of ICRs displaying differential association with two parental alleles.

  10. Allele-specific methylation occurs at genetic variants associated with complex disease.

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    John N Hutchinson

    Full Text Available We hypothesize that the phenomenon of allele-specific methylation (ASM may underlie the phenotypic effects of multiple variants identified by Genome-Wide Association studies (GWAS. We evaluate ASM in a human population and document its genome-wide patterns in an initial screen at up to 380,678 sites within the genome, or up to 5% of the total genomic CpGs. We show that while substantial inter-individual variation exists, 5% of assessed sites show evidence of ASM in at least six samples; the majority of these events (81% are under genetic influence. Many of these cis-regulated ASM variants are also eQTLs in peripheral blood mononuclear cells and monocytes and/or in high linkage-disequilibrium with variants linked to complex disease. Finally, focusing on autoimmune phenotypes, we extend this initial screen to confirm the association of cis-regulated ASM with multiple complex disease-associated variants in an independent population using next-generation bisulfite sequencing. These four variants are implicated in complex phenotypes such as ulcerative colitis and AIDS progression disease (rs10491434, Celiac disease (rs2762051, Crohn's disease, IgA nephropathy and early-onset inflammatory bowel disease (rs713875 and height (rs6569648. Our results suggest cis-regulated ASM may provide a mechanistic link between the non-coding genetic changes and phenotypic variation observed in these diseases and further suggests a route to integrating DNA methylation status with GWAS results.

  11. Assessing allele-specific expression across multiple tissues from RNA-seq read data

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    Pirinen, Matti; Lappalainen, Tuuli; Zaitlen, Noah A.; Dermitzakis, Emmanouil T.; Donnelly, Peter; McCarthy, Mark I.; Rivas, Manuel A.

    2015-01-01

    Motivation: RNA sequencing enables allele-specific expression (ASE) studies that complement standard genotype expression studies for common variants and, importantly, also allow measuring the regulatory impact of rare variants. The Genotype-Tissue Expression (GTEx) project is collecting RNA-seq data on multiple tissues of a same set of individuals and novel methods are required for the analysis of these data. Results: We present a statistical method to compare different patterns of ASE across tissues and to classify genetic variants according to their impact on the tissue-wide expression profile. We focus on strong ASE effects that we are expecting to see for protein-truncating variants, but our method can also be adjusted for other types of ASE effects. We illustrate the method with a real data example on a tissue-wide expression profile of a variant causal for lipoid proteinosis, and with a simulation study to assess our method more generally. Availability and implementation: http://www.well.ox.ac.uk/~rivas/mamba/. R-sources and data examples http://www.iki.fi/mpirinen/ Contact: matti.pirinen@helsinki.fi or rivas@well.ox.ac.uk Supplementary information: Supplementary data are available at Bioinformatics online. PMID:25819081

  12. A majority of Huntington's disease patients may be treatable by individualized allele-specific RNA interference.

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    Lombardi, Maria Stella; Jaspers, Leonie; Spronkmans, Christine; Gellera, Cinzia; Taroni, Franco; Di Maria, Emilio; Donato, Stefano Di; Kaemmerer, William F

    2009-06-01

    Use of RNA interference to reduce huntingtin protein (htt) expression in affected brain regions may provide an effective treatment for Huntington disease (HD), but it remains uncertain whether suppression of both wild-type and mutant alleles in a heterozygous patient will provide more benefit than harm. Previous research has shown suppression of just the mutant allele is achievable using siRNA targeted to regions of HD mRNA containing single nucleotide polymorphisms (SNPs). To determine whether more than a minority of patients may be eligible for an allele-specific therapy, we genotyped DNA from 327 unrelated European Caucasian HD patients at 26 SNP sites in the HD gene. Over 86% of the patients were found to be heterozygous for at least one SNP among those tested. Because the sites are genetically linked, one cannot use the heterozygosity rates of the individual SNPs to predict how many sites (and corresponding allele-specific siRNA) would be needed to provide at least one treatment possibility for this percentage of patients. By computing all combinations, we found that a repertoire of allele-specific siRNA corresponding to seven sites can provide at least one allele-specific siRNA treatment option for 85.6% of our sample. Moreover, we provide evidence that allele-specific siRNA targeting these sites are readily identifiable using a high throughput screening method, and that allele-specific siRNA identified using this method indeed show selective suppression of endogenous mutant htt protein in fibroblast cells from HD patients. Therefore, allele-specific siRNA are not so rare as to be impractical to find and use therapeutically.

  13. Allele-specific PCR detection of sweet cherry self-incompatibility (S) alleles S1 to S16 using consensus and allele-specific primers.

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    Sonneveld, T; Tobutt, K R; Robbins, T P

    2003-10-01

    PCR-based identification of all 13 known self-incompatibility (S) alleles of sweet cherry is reported. Two pairs of consensus primers were designed from our previously published cDNA sequences of S(1) to S(6) S-RNases, the stylar components of self-incompatibility, to reveal length variation of the first and the second introns. With the exception of the first intron of S(13), these also amplified S(7) to S(14) and an allele previously referred to as S(x), which we now label S(16). The genomic PCR products were cloned and sequenced. The partial sequence of S(11) matched that of S(7) and the alleles were shown to have the same functional specificity. Allele-specific primers were designed for S(7) to S(16), so that allele-specific primers are now available for all 13 S alleles of cherry (S(8), S(11) and S(15) are duplicates). These can be used to distinguish between S alleles with introns of similar size and to confirm genotypes determined with consensus primers. The reliability of the PCR with allele-specific primers was improved by the inclusion of an internal control. The use of the consensus and allele-specific primers was demonstrated by resolving conflicting genotypes that have been published recently and by determining genotypes of 18 new cherry cultivars. Two new groups are proposed, Group XXIII (S(3) S(16)), comprising 'Rodmersham Seedling' and 'Strawberry Heart', and Group XXIV (S(6) S(12)), comprising 'Aida' and 'Flamentiner'. Four new self-compatibility genotypes, S(3) S(3)', S(4)' S(6), S(4)' S(9) and S(4)' S(13), were found. The potential use of the consensus primers to reveal incompatibility alleles in other cherry species is also demonstrated.

  14. Epidemiological survey of Theileria parasite infection of cattle in Northeast China by allele-specific PCR.

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    Yu, Longzheng; Zhang, Shoufa; Liang, Wanfeng; Jin, Chunmei; Jia, Lijun; Luo, Yuzi; Li, Yan; Cao, Shinuo; Yamagishi, Junya; Nishikawa, Yoshifumi; Kawano, Suguru; Fujisaki, Kozo; Xuan, Xuenan

    2011-11-01

    An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.

  15. Simultaneous SNP identification and assessment of allele-specific bias from ChIP-seq data

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    Ni Yunyun

    2012-09-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs have been associated with many aspects of human development and disease, and many non-coding SNPs associated with disease risk are presumed to affect gene regulation. We have previously shown that SNPs within transcription factor binding sites can affect transcription factor binding in an allele-specific and heritable manner. However, such analysis has relied on prior whole-genome genotypes provided by large external projects such as HapMap and the 1000 Genomes Project. This requirement limits the study of allele-specific effects of SNPs in primary patient samples from diseases of interest, where complete genotypes are not readily available. Results In this study, we show that we are able to identify SNPs de novo and accurately from ChIP-seq data generated in the ENCODE Project. Our de novo identified SNPs from ChIP-seq data are highly concordant with published genotypes. Independent experimental verification of more than 100 sites estimates our false discovery rate at less than 5%. Analysis of transcription factor binding at de novo identified SNPs revealed widespread heritable allele-specific binding, confirming previous observations. SNPs identified from ChIP-seq datasets were significantly enriched for disease-associated variants, and we identified dozens of allele-specific binding events in non-coding regions that could distinguish between disease and normal haplotypes. Conclusions Our approach combines SNP discovery, genotyping and allele-specific analysis, but is selectively focused on functional regulatory elements occupied by transcription factors or epigenetic marks, and will therefore be valuable for identifying the functional regulatory consequences of non-coding SNPs in primary disease samples.

  16. Adoption Patterns for the Digital Post System by Danish Municipalities and Citizens

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    Berger, Jesper B.; Hertzum, Morten

    2014-01-01

    The value of e-government, services to citizens by public institutions through the internet, is dependent on the mutual adoption of e-government by both the public institution and the citizens. This paper describes a longitudinal study of e-government adoption by municipalities and citizens...... in Denmark. We studied the e-government initiative Digital Post ¬¬– encrypted digital communication between municipalities and citizens. Over the three-year adoption period, we found four adoption patterns among municipalities, characterized by a slow, late, gradual or early increase in the use of Digital...

  17. Ising-like agent-based technology diffusion model: adoption patterns vs. seeding strategies

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    Laciana, Carlos E

    2010-01-01

    The well-known Ising model used in statistical physics was adapted to a social dynamics context to simulate the adoption of a technological innovation. The model explicitly combines (a) an individual's perception of the advantages of an innovation and (b) social influence from members of the decision-maker's social network. The micro-level adoption dynamics are embedded into an agent-based model that allows exploration of macro-level patterns of technology diffusion throughout systems with different configurations (number and distributions of early adopters, social network topologies). In the present work we carry out many numerical simulations. We find that when the gap between the individual's perception of the options is high, the adoption speed increases if the dispersion of early adopters grows. Another test was based on changing the network topology by means of stochastic connections to a common opinion reference (hub), which resulted in an increment in the adoption speed. Finally, we performed a simula...

  18. BaalChIP: Bayesian analysis of allele-specific transcription factor binding in cancer genomes.

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    de Santiago, Ines; Liu, Wei; Yuan, Ke; O'Reilly, Martin; Chilamakuri, Chandra Sekhar Reddy; Ponder, Bruce A J; Meyer, Kerstin B; Markowetz, Florian

    2017-02-24

    Allele-specific measurements of transcription factor binding from ChIP-seq data are key to dissecting the allelic effects of non-coding variants and their contribution to phenotypic diversity. However, most methods of detecting an allelic imbalance assume diploid genomes. This assumption severely limits their applicability to cancer samples with frequent DNA copy-number changes. Here we present a Bayesian statistical approach called BaalChIP to correct for the effect of background allele frequency on the observed ChIP-seq read counts. BaalChIP allows the joint analysis of multiple ChIP-seq samples across a single variant and outperforms competing approaches in simulations. Using 548 ENCODE ChIP-seq and six targeted FAIRE-seq samples, we show that BaalChIP effectively corrects allele-specific analysis for copy-number variation and increases the power to detect putative cis-acting regulatory variants in cancer genomes.

  19. Allele-specific amplification and electrochemiluminescence method for single nucleotide polymorphism analysis

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    A new approach combined the specificity of allele-specific amplification (ASA) with the sensitivity of electrochemiluminescence (ECL) assay for single nucleotide polymorphism (SNP) analysis was proposed. Briefly, target gene was amplified by a biotin-labeled allele-specific forward primer and a Ru(bpy)32+ (TBR)-labeled universal reverse primer. Then, the amplicon was captured onto streptavidin-coated paramagnetic beads through biotin label, and detected by measuring the ECL signal of TBR label. Different genotypes were distinguished according to the ECL values of the amplicons by different genotypic primers. K-ras oncogene was used as a target to validate the feasibility of the method. The experiment results show that the different genotypes can be clearly distinguished by ASA-ECL assay. The method is useful in SNP analysis due to its sensitivity,safety, and simplicity.(C) 2007 Da Xing. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved.

  20. Determination of DQB1 alleles using PCR amplification and allele-specific primers.

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    Lepage, V; Ivanova, R; Loste, M N; Mallet, C; Douay, C; Naoumova, E; Charron, D

    1995-10-01

    Molecular genotyping of HLA class II genes is commonly carried out using polymerase chain reaction (PCR) in combination with sequence-specific oligotyping (PCR-SSO) or a combination of the PCR and restriction fragment length polymorphism methods (PCR-RFLP). However, the identification of the DQB1 type by PCR-SSO and PCR-RFLP is very time-consuming which is disadvantageous for the typing of cadaveric organ donors. We have developed a DQB1 typing method using PCR in combination with allele-specific amplification (PCR-ASA), which allows the identification of the 17 most frequent alleles in one step using seven amplification mixtures. PCR allele-specific amplification HLA-DQB1 typing is easy to perform, and the results are easy to interpret in routine clinical practice. The PCR-ASA method is therefore better suited to DQB1 typing for organ transplantation than other methods.

  1. High-speed droplet-allele-specific polymerase chain reaction for genotyping of single nucleotide polymorphisms.

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    Matsuda, Kazuyuki; Honda, Takayuki

    2015-01-01

    Single nucleotide alternations such as single nucleotide polymorphisms (SNPs) or single nucleotide mutations are useful genetic markers for molecular diagnosis, prognosis, drug response, and predisposition to diseases. Rapid identification of SNPs or mutations is clinically important, especially for determining drug responses and selection of molecular-targeted therapy. Here, we describe a rapid genotyping assay based on the allele-specific polymerase chain reaction (AS-PCR) by using our droplet-PCR machine (droplet-AS-PCR).

  2. Allele-Specific Quantitative PCR for Accurate, Rapid, and Cost-Effective Genotyping.

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    Lee, Han B; Schwab, Tanya L; Koleilat, Alaa; Ata, Hirotaka; Daby, Camden L; Cervera, Roberto Lopez; McNulty, Melissa S; Bostwick, Hannah S; Clark, Karl J

    2016-06-01

    Customizable endonucleases such as transcription activator-like effector nucleases (TALENs) and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) enable rapid generation of mutant strains at genomic loci of interest in animal models and cell lines. With the accelerated pace of generating mutant alleles, genotyping has become a rate-limiting step to understanding the effects of genetic perturbation. Unless mutated alleles result in distinct morphological phenotypes, mutant strains need to be genotyped using standard methods in molecular biology. Classic restriction fragment length polymorphism (RFLP) or sequencing is labor-intensive and expensive. Although simpler than RFLP, current versions of allele-specific PCR may still require post-polymerase chain reaction (PCR) handling such as sequencing, or they are more expensive if allele-specific fluorescent probes are used. Commercial genotyping solutions can take weeks from assay design to result, and are often more expensive than assembling reactions in-house. Key components of commercial assay systems are often proprietary, which limits further customization. Therefore, we developed a one-step open-source genotyping method based on quantitative PCR. The allele-specific qPCR (ASQ) does not require post-PCR processing and can genotype germline mutants through either threshold cycle (Ct) or end-point fluorescence reading. ASQ utilizes allele-specific primers, a locus-specific reverse primer, universal fluorescent probes and quenchers, and hot start DNA polymerase. Individual laboratories can further optimize this open-source system as we completely disclose the sequences, reagents, and thermal cycling protocol. We have tested the ASQ protocol to genotype alleles in five different genes. ASQ showed a 98-100% concordance in genotype scoring with RFLP or Sanger sequencing outcomes. ASQ is time-saving because a single qPCR without post-PCR handling suffices to score

  3. Allele-specific enzymatic amplification of. beta. -globin genomic DNA for diagnosis of sickle cell anemia

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    Wu, D.Y.; Ugozzoli, L.; Pal, B.K.; Wallace, B. (Beckman Research Institute of the City of Hope, Duarte, CA (USA))

    1989-04-01

    A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell {beta}-globin allele in genomic DNA without additional steps of probe hybridization, ligation, or restriction enzyme cleavage. Two allele-specific oligonucleotide primers, one specific for the sickle cell allele and one specific for the normal allele, together with another primer complementary to both alleles were used in the polymerase chain reaction with genomic DNA templates. The allele-specific primers differed from each other in their terminal 3{prime} nucleotide. Under the proper annealing temperature and polymerase chain reaction conditions, these primers only directed amplification on their complementary allele. In a single blind study of DNA samples from 12 individuals, this method correctly and unambiguously allowed for the determination of the genotypes with no false negatives or positives. If ASPCR is able to discriminate all allelic variation (both transition and transversion mutations), this method has the potential to be a powerful approach for genetic disease diagnosis, carrier screening, HLA typing, human gene mapping, forensics, and paternity testing.

  4. Quantification of allele-specific expression of a gene encoding strawberry polygalacturonase-inhibiting protein (PGIP) using Pyrosequencing((TM))

    NARCIS (Netherlands)

    Schaart, J.G.; Mehli, L.; Schouten, H.J.

    2005-01-01

    Recent studies indicate that allele-specific differences in gene expression are a common phenomenon. The extent to which differential allelic expression exists might be underestimated, due to the limited accuracy of the methods used so far. To demonstrate allele-specific expression, we investigated

  5. A genome-wide screen in human embryonic stem cells reveals novel sites of allele-specific histone modification associated with known disease loci

    LENUS (Irish Health Repository)

    Prendergast, James G D

    2012-05-19

    AbstractBackgroundChromatin structure at a given site can differ between chromosome copies in a cell, and such imbalances in chromatin structure have been shown to be important in understanding the molecular mechanisms controlling several disease loci. Human genetic variation, DNA methylation, and disease have been intensely studied, uncovering many sites of allele-specific DNA methylation (ASM). However, little is known about the genome-wide occurrence of sites of allele-specific histone modification (ASHM) and their relationship to human disease. The aim of this study was to investigate the extent and characteristics of sites of ASHM in human embryonic stem cells (hESCs).ResultsUsing a statistically rigorous protocol, we investigated the genomic distribution of ASHM in hESCs, and their relationship to sites of allele-specific expression (ASE) and DNA methylation. We found that, although they were rare, sites of ASHM were substantially enriched at loci displaying ASE. Many were also found at known imprinted regions, hence sites of ASHM are likely to be better markers of imprinted regions than sites of ASM. We also found that sites of ASHM and ASE in hESCs colocalize at risk loci for developmental syndromes mediated by deletions, providing insights into the etiology of these disorders.ConclusionThese results demonstrate the potential importance of ASHM patterns in the interpretation of disease loci, and the protocol described provides a basis for similar studies of ASHM in other cell types to further our understanding of human disease susceptibility.

  6. Accurate quantitation of allele-specific expression patterns by analysis of DNA melting

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    Jeong, Sangkyun; Hahn, Yoonsoo; Rong, Qi; Pfeifer, Karl

    2007-01-01

    Epigenetic and genetic mechanisms can result in large differences in expression levels of the two alleles in a diploid organism. Furthermore, these differences may be critical to phenotypic variations among individuals. In this study, we present a novel procedure and algorithm to precisely and accurately quantitate the relative expression of each allele. This method uses the differential melting properties of DNAs differing at even a single base pair. By referring to the melting characteristi...

  7. Allele-specific amplification in cancer revealed by SNP array analysis.

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    Thomas LaFramboise

    2005-11-01

    Full Text Available Amplification, deletion, and loss of heterozygosity of genomic DNA are hallmarks of cancer. In recent years a variety of studies have emerged measuring total chromosomal copy number at increasingly high resolution. Similarly, loss-of-heterozygosity events have been finely mapped using high-throughput genotyping technologies. We have developed a probe-level allele-specific quantitation procedure that extracts both copy number and allelotype information from single nucleotide polymorphism (SNP array data to arrive at allele-specific copy number across the genome. Our approach applies an expectation-maximization algorithm to a model derived from a novel classification of SNP array probes. This method is the first to our knowledge that is able to (a determine the generalized genotype of aberrant samples at each SNP site (e.g., CCCCT at an amplified site, and (b infer the copy number of each parental chromosome across the genome. With this method, we are able to determine not just where amplifications and deletions occur, but also the haplotype of the region being amplified or deleted. The merit of our model and general approach is demonstrated by very precise genotyping of normal samples, and our allele-specific copy number inferences are validated using PCR experiments. Applying our method to a collection of lung cancer samples, we are able to conclude that amplification is essentially monoallelic, as would be expected under the mechanisms currently believed responsible for gene amplification. This suggests that a specific parental chromosome may be targeted for amplification, whether because of germ line or somatic variation. An R software package containing the methods described in this paper is freely available at http://genome.dfci.harvard.edu/~tlaframb/PLASQ.

  8. Allele-specific silencing of mutant Ataxin-7 in SCA7 patient-derived fibroblasts.

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    Scholefield, Janine; Watson, Lauren; Smith, Danielle; Greenberg, Jacquie; Wood, Matthew J A

    2014-12-01

    Polyglutamine (polyQ) disorders are inherited neurodegenerative conditions defined by a common pathogenic CAG repeat expansion leading to a toxic gain-of-function of the mutant protein. Consequences of this toxicity include activation of heat-shock proteins (HSPs), impairment of the ubiquitin-proteasome pathway and transcriptional dysregulation. Several studies in animal models have shown that reducing levels of toxic protein using small RNAs would be an ideal therapeutic approach for such disorders, including spinocerebellar ataxia-7 (SCA7). However, testing such RNA interference (RNAi) effectors in genetically appropriate patient cell lines with a disease-relevant phenotype has yet to be explored. Here, we have used primary adult dermal fibroblasts from SCA7 patients and controls to assess the endogenous allele-specific silencing of ataxin-7 by two distinct siRNAs. We further identified altered expression of two disease-relevant transcripts in SCA7 patient cells: a twofold increase in levels of the HSP DNAJA1 and a twofold decrease in levels of the de-ubiquitinating enzyme, UCHL1. After siRNA treatment, the expression of both genes was restored towards normal levels. To our knowledge, this is the first time that allele-specific silencing of mutant ataxin-7, targeting a common SNP, has been demonstrated in patient cells. These findings highlight the advantage of an allele-specific RNAi-based therapeutic approach, and indicate the value of primary patient-derived cells as useful models for mechanistic studies and for measuring efficacy of RNAi effectors on a patient-to-patient basis in the polyQ diseases.

  9. Genome-wide survey of allele-specific splicing in humans

    Directory of Open Access Journals (Sweden)

    Scheffler Konrad

    2008-06-01

    Full Text Available Abstract Background Accurate mRNA splicing depends on multiple regulatory signals encoded in the transcribed RNA sequence. Many examples of mutations within human splice regulatory regions that alter splicing qualitatively or quantitatively have been reported and allelic differences in mRNA splicing are likely to be a common and important source of phenotypic diversity at the molecular level, in addition to their contribution to genetic disease susceptibility. However, because the effect of a mutation on the efficiency of mRNA splicing is often difficult to predict, many mutations that cause disease through an effect on splicing are likely to remain undiscovered. Results We have combined a genome-wide scan for sequence polymorphisms likely to affect mRNA splicing with analysis of publicly available Expressed Sequence Tag (EST and exon array data. The genome-wide scan uses published tools and identified 30,977 SNPs located within donor and acceptor splice sites, branch points and exonic splicing enhancer elements. For 1,185 candidate splicing polymorphisms the difference in splicing between alternative alleles was corroborated by publicly available exon array data from 166 lymphoblastoid cell lines. We developed a novel probabilistic method to infer allele-specific splicing from EST data. The method uses SNPs and alternative mRNA isoforms mapped to EST sequences and models both regulated alternative splicing as well as allele-specific splicing. We have also estimated heritability of splicing and report that a greater proportion of genes show evidence of splicing heritability than show heritability of overall gene expression level. Our results provide an extensive resource that can be used to assess the possible effect on splicing of human polymorphisms in putative splice-regulatory sites. Conclusion We report a set of genes showing evidence of allele-specific splicing from an integrated analysis of genomic polymorphisms, EST data and exon array

  10. Therapy for dominant inherited diseases by allele-specific RNA interference: successes and pitfalls.

    Science.gov (United States)

    Trochet, Delphine; Prudhon, Bernard; Vassilopoulos, Stéphane; Bitoun, Marc

    2015-01-01

    RNA interference (RNAi) is a conserved mechanism for post-transcriptional gene silencing mediated by messenger RNA (mRNA) degradation. RNAi is commonly induced by synthetic siRNA or shRNA which recognizes the targeted mRNA by base pairing and leads to target-mRNA degradation. RNAi may discriminate between two sequences only differing by one nucleotide conferring a high specificity of RNAi for its target mRNA. This property was used to develop a particular therapeutic strategy called "allele-specific-RNA interference" devoted to silence the mutated allele of genes causing dominant inherited diseases without affecting the normal allele. Therapeutic benefit was now demonstrated in cells from patients and animal models, and promising results of the first phase Ib clinical trial using siRNA-based allele-specific therapy were reported in Pachyonychia Congenita, an inherited skin disorder due to dominant mutations in the Keratin 6 gene. Our purpose is to review the successes of this strategy aiming to treat dominant inherited diseases and to highlight the pitfalls to avoid.

  11. Optimized Multiplex Detection of 7 KRAS Mutations by Taqman Allele-Specific qPCR

    Science.gov (United States)

    Orue, Andrea; Rieber, Manuel

    2016-01-01

    Establishing the KRAS mutational status of tumor samples is essential to manage patients with colorectal or lung cancer, since these mutations preclude treatment with monoclonal anti-epidermal growth factor receptor (EGFR) antibodies. We report an inexpensive, rapid multiplex allele-specific qPCR method detecting the 7 most clinically relevant KRAS somatic mutations with concomitant amplification of non-mutated KRAS in tumor cells and tissues from CRC patients. Positive samples evidenced in the multiplex assay were further subjected to individual allele-specific analysis, to define the specific mutation. Reference human cancer DNA harbouring either G12A, G12C, G12D, G12R, G12S, G12V and G13D confirmed assay specificity with ≤1% sensitivity of mutant alleles. KRAS multiplex mutation analysis usefulness was also demonstrated with formalin-fixed paraffin embedded (FFPE) from CRC biopsies. Conclusion. Co-amplification of non-mutated DNA avoided false negatives from degraded samples. Moreover, this cost effective assay is compatible with mutation detection by DNA sequencing in FFPE tissues, but with a greater sensitivity when mutant DNA concentrations are limiting. PMID:27632281

  12. Germline allele-specific expression of DAPK1 in chronic lymphocytic leukemia.

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    Quan-Xiang Wei

    Full Text Available We previously reported a rare germline variant (c.1-6531 that resulted in allele-specific expression (ASE of death-associated protein kinase 1 (DAPK1 and predisposition to chronic lymphocytic leukemia (CLL. We investigated a cohort of CLL patients lacking this mutation for the presence of ASE of DAPK1. We developed a novel strategy that combines single-nucleotide primer extension (SNuPE with MALDI-TOF mass spectrometry, and detected germline DAPK1 ASE in 17 out of 120 (14.2% CLL patients associated with a trend towards younger age at diagnosis. ASE was absent in 63 healthy controls. Germline cells of CLL patients with ASE showed increased levels of DNA methylation in the promoter region, however, neither genetic nor further epigenetic aberrations could be identified in the DAPK1 5' upstream regulatory region, within distinct exons or in the 3'-UTR. We identified B-lymphoid malignancy related cell line models harboring allelic imbalance and found that allele-specific methylation in DAPK1 is associated with ASE. Our data indicate that ASE at the DAPK1 gene locus is a recurrent event, mediated by epigenetic mechanisms and potentially predisposing to CLL.

  13. A molecular method for S-allele identification in apple based on allele-specific PCR.

    Science.gov (United States)

    Janssens, G A; Goderis, I J; Broekaert, W F; Broothaerts, W

    1995-09-01

    cDNA sequences corresponding to two self-incompatibility alleles (S-alleles) of the apple cv 'Golden Delicious' have previously been described, and now we report the identification of three additional S-allele cDNAs of apple, one of which was isolated from a pistil cDNA library of cv 'Idared' and two of which were obtained by reverse transcription-PCR (RT-PCR) on pistil RNA of cv 'Queen's Cox'. A comparison of the deduced amino acid sequences of these five S-allele cDNAs revealed an average homology of 69%. Based on the nucleotide sequences of these S-allele cDNAs, we developed a molecular technique for the diagnostic identification of the five different S-alleles in apple cultivars. The method used consists of allele-specific PCR amplification of genomic DNA followed by digestion of the amplification product with an allele-specific restriction endonuclease. Analysis of a number of apple cultivars with known S-phenotype consistently showed coincidence of phenotypic and direct molecular data of the S-allele constitution of the cultivars. It is concluded that the S-allele identification approach reported here provides a rapid and useful method to determine the S-genotype of apple cultivars.

  14. Visualizing allele-specific expression in single cells reveals epigenetic mosaicism in an H19 loss-of-imprinting mutant.

    Science.gov (United States)

    Ginart, Paul; Kalish, Jennifer M; Jiang, Connie L; Yu, Alice C; Bartolomei, Marisa S; Raj, Arjun

    2016-03-01

    Imprinting is a classic mammalian epigenetic phenomenon that results in expression from a single parental allele. Imprinting defects can lead to inappropriate expression from the normally silenced allele, but it remains unclear whether every cell in a mutant organism follows the population average, which would have profound implications for human imprinting disorders. Here, we apply a new fluorescence in situ hybridization method that measures allele-specific expression in single cells to address this question in mutants exhibiting aberrant H19/Igf2 (insulin-like growth factor 2) imprinting. We show that mutant primary embryonic mouse fibroblasts are comprised of two subpopulations: one expressing both H19 alleles and another expressing only the maternal copy. Only in the latter cell population is Igf2 expression detected. Furthermore, the two subpopulations are stable in that cells do not interconvert between the two expression patterns. Combined small input methylation analysis and transcriptional imaging revealed that these two mutant subpopulations exhibit distinct methylation patterns at their imprinting control regions. Consistently, pharmacological inhibition of DNA methylation reduced the proportion of monoallelic cells. Importantly, we observed that the same two subpopulations are also present in vivo within murine cardiac tissue. Our results establish that imprinting disorders can display striking single-cell heterogeneity in their molecular phenotypes and suggest that such heterogeneity may underlie epigenetic mosaicism in human imprinting disorders.

  15. Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.

    OpenAIRE

    1989-01-01

    A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, ligation, or restriction enzyme cleavage. Two allele-specific oligonucleotide primers, one specific for the sickle cell allele and one specific for the normal allele, together with another primer co...

  16. High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta

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    Clark Taane G

    2010-04-01

    Full Text Available Abstract Background Imprinted genes show expression from one parental allele only and are important for development and behaviour. This extreme mode of allelic imbalance has been described for approximately 56 human genes. Imprinting status is often disrupted in cancer and dysmorphic syndromes. More subtle variation of gene expression, that is not parent-of-origin specific, termed 'allele-specific gene expression' (ASE is more common and may give rise to milder phenotypic differences. Using two allele-specific high-throughput technologies alongside bioinformatics predictions, normal term human placenta was screened to find new imprinted genes and to ascertain the extent of ASE in this tissue. Results Twenty-three family trios of placental cDNA, placental genomic DNA (gDNA and gDNA from both parents were tested for 130 candidate genes with the Sequenom MassArray system. Six genes were found differentially expressed but none imprinted. The Illumina ASE BeadArray platform was then used to test 1536 SNPs in 932 genes. The array was enriched for the human orthologues of 124 mouse candidate genes from bioinformatics predictions and 10 human candidate imprinted genes from EST database mining. After quality control pruning, a total of 261 informative SNPs (214 genes remained for analysis. Imprinting with maternal expression was demonstrated for the lymphocyte imprinted gene ZNF331 in human placenta. Two potential differentially methylated regions (DMRs were found in the vicinity of ZNF331. None of the bioinformatically predicted candidates tested showed imprinting except for a skewed allelic expression in a parent-specific manner observed for PHACTR2, a neighbour of the imprinted PLAGL1 gene. ASE was detected for two or more individuals in 39 candidate genes (18%. Conclusions Both Sequenom and Illumina assays were sensitive enough to study imprinting and strong allelic bias. Previous bioinformatics approaches were not predictive of new imprinted genes

  17. Allele-specific interactions between CAST AWAY and NEVERSHED control abscission in Arabidopsis flowers

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    William D. Groner

    2016-10-01

    Full Text Available An advantage of analyzing abscission in genetically tractable model plants is the ability to make use of classic genetic tools such as suppression analysis. We have investigated the regulation of organ abscission by carrying out suppression analysis in Arabidopsis flowers. Plants carrying mutations in the NEVERSHED (NEV gene, which encodes an ADP-ribosylation factor GTPase-activating protein, retain their outer floral organs after fertilization. Mutant alleles of CAST AWAY (CST, which encodes a receptor-like cytoplasmic kinase, were found to restore organ abscission in nev flowers in an allele-specific manner. To further explore the basis of the interactions between CST and NEV, we tested whether the site of a nev mutation is predictive of its ability to be suppressed. Our results suggest instead that the strength of a nev allele influences whether organ abscission can be rescued by a specific allele of CST.

  18. Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic

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    Eleonidas Moura Lima

    2015-06-01

    Full Text Available Gastric cancer (GC is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP rs1695, in the Glutathione S-Transferase Pi (GSTP1 gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ2 = 9.7, P 0.05. These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.

  19. Identification of transcriptome SNPs for assessing allele-specific gene expression in a super-hybrid rice Xieyou9308.

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    Rongrong Zhai

    Full Text Available Hybridization, a common process in nature, can give rise to a vast reservoir of allelic variants. Combination of these allelic variants may result in novel patterns of gene action and is thought to contribute to heterosis. In this study, we analyzed genome-wide allele-specific gene expression (ASGE in the super-hybrid rice variety Xieyou9308 using RNA sequencing technology (RNA-Seq. We identified 9325 reliable single nucleotide polymorphisms (SNPs distributed throughout the genome. Nearly 68% of the identified polymorphisms were CT and GA SNPs between R9308 and Xieqingzao B, suggesting the existence of DNA methylation, a heritable epigenetic mark, in the parents and their F1 hybrid. Of 2793 identified transcripts with consistent allelic biases, only 480 (17% showed significant allelic biases during tillering and/or heading stages, implying that trans effects may mediate most transcriptional differences in hybrid offspring. Approximately 67% and 62% of the 480 transcripts showed R9308 allelic expression biases at tillering and heading stages, respectively. Transcripts with higher levels of gene expression in R9308 also exhibited R9308 allelic biases in the hybrid. In addition, 125 transcripts were identified with significant allelic expression biases at both stages, of which 74% showed R9308 allelic expression biases. R9308 alleles may tend to preserve their characteristic states of activity in the hybrid and may play important roles in hybrid vigor at both stages. The allelic expression of 355 transcripts was highly stage-specific, with divergent allelic expression patterns observed at different developmental stages. Many transcripts associated with stress resistance were differently regulated in the F1 hybrid. The results of this study may provide valuable insights into molecular mechanisms of heterosis.

  20. Utilising polymorphisms to achieve allele-specific genome editing in zebrafish

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    Samuel J. Capon

    2017-01-01

    Full Text Available The advent of genome editing has significantly altered genetic research, including research using the zebrafish model. To better understand the selectivity of the commonly used CRISPR/Cas9 system, we investigated single base pair mismatches in target sites and examined how they affect genome editing in the zebrafish model. Using two different zebrafish strains that have been deep sequenced, CRISPR/Cas9 target sites containing polymorphisms between the two strains were identified. These strains were crossed (creating heterozygotes at polymorphic sites and CRISPR/Cas9 complexes that perfectly complement one strain injected. Sequencing of targeted sites showed biased, allele-specific editing for the perfectly complementary sequence in the majority of cases (14/19. To test utility, we examined whether phenotypes generated by F0 injection could be internally controlled with such polymorphisms. Targeting of genes bmp7a and chordin showed reduction in the frequency of phenotypes in injected ‘heterozygotes’ compared with injecting the strain with perfect complementarity. Next, injecting CRISPR/Cas9 complexes targeting two separate sites created deletions, but deletions were biased to selected chromosomes when one CRISPR/Cas9 target contained a polymorphism. Finally, integration of loxP sequences occurred preferentially in alleles with perfect complementarity. These experiments demonstrate that single nucleotide polymorphisms (SNPs present throughout the genome can be utilised to increase the efficiency of in cis genome editing using CRISPR/Cas9 in the zebrafish model.

  1. Varietal Preferences and Adoption Pattern of Economically Viable Medicinal and Aromatic Crops by the Indian Farmers

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    H. K. Yadav

    2013-03-01

    Full Text Available Central Institute of Medicinal and Aromatic Plants (CIMAP is the knowledge gateway of medicinal andaromatic plants related services and technologies. It organizes kisan mela (Farmers Fair every year anddisplay new varieties/technologies and innovative cultivation practices on commercially viable MAPs forits end users. Analyzed data reveals that 75.85% farmer preferring aromatic crops and only 24.15% farmersin favor of medicinal crops. In the year 2010-2012, aromatic crops are quite popular among the farmersand increment pattern of aromatic crops were 40% & 71.90 in Mint; 48% & 78.37% in Rose, Menthol mintstill dominates the choice, while in case of medicinal plants, the adoption pattern increments were 75% &38.09% in Stevia; 35.71% & 21.05% in Withania; 37.5% & 77.27% in Tulsi respectively. It leads to improvedsocio-economic condition of farmers in the area using cultivable land but also in rural sector under stress andunsustainable land.

  2. Imprinted chromosomal domains revealed by allele-specific replication timing of the GABRB3 and GABRA5 genes

    Energy Technology Data Exchange (ETDEWEB)

    LaSalle, J.; Flint, A.; Lalande, M. [Harvard Medical School, Boston, MA (United States)] [and others

    1994-09-01

    The GABRB3 and GABRA5 genes are organized as a cluster in chromosome 15q11-q13. The genes are separated by around 100 kb and arranged in opposite transcriptional orientations. The GABA{sub A} receptor cluster lies near the Angelman and Prader-Willi loci and displays asynchronous DNA replication, suggesting that this region is subject to parental imprinting. In order to further study the association between DNA replication and imprinting, allele-specific replication was assayed by fluorescence in situ hybridization with {lambda}-phage probes from the GABRB3/A5 region and a D15Z1 satellite probe to identify the parental origin of each chromosome. The replication kinetics of each allele was determined by using a flow sorter to fractionate mitogen-stimulated lymphocytes on the basis of cell cycle progression prior to FISH analysis. These kinetic studies reveal a 50-150 kb chromosomal domain extending from the middle of the GABRB3/A5 intergenic region into the GABRA5 5{prime}-UTR which displays maternal replication in early S with paternal replication delayed until the end of S. In contrast, genomic regions on either side of this maternal early replication domain exhibit the opposite pattern with paternal before maternal replication and both alleles replicating in the latter half of S. These results indicate that the GABRB3/A5 region is divided into domains in which replication timing is determined by parental origin. In addition to a loss of asynchronous replication, organization into replication timing domains is also lost in lymphocytes from maternal and paternal uniparental disomy 15 patients suggesting that a chromosome contribution from both parents is required for the establishment of the imprinted replication domains.

  3. Self-(in)compatibility inheritance and allele-specific marker development in yellow mustard (Sinapis alba).

    Science.gov (United States)

    Zeng, Fangqin; Cheng, Bifang

    2014-01-01

    Yellow mustard (Sinapis alba) has a sporophytic self-incompatibility reproduction system. Genetically stable self-incompatible (SI) and self-compatible (SC) inbred lines have recently been developed in this crop. Understanding the S haplotype of different inbred lines and the inheritance of the self-(in)compatibility (SI/SC) trait is very important for breeding purposes. In this study, we used the S-locus gene-specific primers in Brassica rapa and Brassica oleracea to clone yellow mustard S-locus genes of SI lines Y514 and Y1130 and SC lines Y1499 and Y1501. The PCR amplification results and DNA sequences of the S-locus genes revealed that Y514 carried the class I S haplotype, while Y1130, Y1499, and Y1501 had the class II S haplotype. The results of our genetic studies indicated that self-incompatibility was dominant over self-compatibility and controlled by a one-gene locus in the two crosses of Y514 × Y1499 and Y1130 × Y1501. Of the five S-locus gene polymorphic primer pairs, Sal-SLGI and Sal-SRKI each generated one dominant marker for the SI phenotype of Y514; Sal-SLGII and Sal-SRKII produced dominant marker(s) for the SC phenotype of Y1501 and Y1499; Sal-SP11II generated one dominant marker for Y1130. These markers co-segregated with the SI/SC phenotype in the F2 populations of the two crosses. In addition, co-dominant markers were developed by mixing the two polymorphic primer pairs specific for each parent in the multiplex PCR, which allowed zygosity to be determined in the F2 populations. The SI/SC allele-specific markers have proven to be very useful for the selection of the desirable SC genotypes in our yellow mustard breeding program.

  4. Efficient and allele-specific genome editing of disease loci in human iPSCs.

    Science.gov (United States)

    Smith, Cory; Abalde-Atristain, Leire; He, Chaoxia; Brodsky, Brett R; Braunstein, Evan M; Chaudhari, Pooja; Jang, Yoon-Young; Cheng, Linzhao; Ye, Zhaohui

    2015-03-01

    Efficient and precise genome editing is crucial for realizing the full research and therapeutic potential of human induced pluripotent stem cells (iPSCs). Engineered nucleases including CRISPR/Cas9 and transcription activator like effector nucleases (TALENs) provide powerful tools for enhancing gene-targeting efficiency. In this study, we investigated the relative efficiencies of CRISPR/Cas9 and TALENs in human iPSC lines for inducing both homologous donor-based precise genome editing and nonhomologous end joining (NHEJ)-mediated gene disruption. Significantly higher frequencies of NHEJ-mediated insertions/deletions were detected at several endogenous loci using CRISPR/Cas9 than using TALENs, especially at nonexpressed targets in iPSCs. In contrast, comparable efficiencies of inducing homologous donor-based genome editing were observed at disease-associated loci in iPSCs. In addition, we investigated the specificity of guide RNAs used in the CRISPR/Cas9 system in targeting disease-associated point mutations in patient-specific iPSCs. Using myeloproliferative neoplasm patient-derived iPSCs that carry an acquired JAK2-V617F point mutation and α1-antitrypsin (AAT) deficiency patient-derived iPSCs that carry an inherited Z-AAT point mutation, we demonstrate that Cas9 can specifically target either the mutant or the wild-type allele with little disruption at the other allele differing by a single nucleotide. Overall, our results demonstrate the advantages of the CRISPR/Cas9 system in allele-specific genome targeting and in NHEJ-mediated gene disruption.

  5. Social media and mobile communications adoption patterns of South African civil society organisations

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    Kiru Pillay

    2014-03-01

    Full Text Available Background: The resurgence of civil society has largely been attributed to the sector’s ability to exploit new interactive technologies and its ability to adapt its communication and mobilisation strategies.Objectives: This study focuses on how South African civil society organisations (CSOs deploy Web 2.0 services and technologies for social advocacy and the context of this technology use.Whilst the literature points to many studies relating to the use of the Internet for advocacy, it also suggests that the role and impact of emerging technologies have not been studied in any detail in CSOs. Such studies have the potential to provide new perspectives to current theoretical frameworks and also to add to the discourse around the use of emerging technologies for advocacy.Method: A survey of South African CSOs explored the level of knowledge of social media services and revealed which services in particular were being adopted.Results: The key findings that emerged were that the sector has a low level of knowledge of social media services and an accompanying low level of adoption. These are partly explained by factors such as macro-economic policies and low levels of Internet penetration and ICT readiness.Conclusion: Further research to determine why certain social media services have been embraced more willingly than others and an analysis of the patterns of adoption to determine any underlying significance or relationships is necessary. An analysis of how CSOs build their advocacy capabilities by appropriating social media and how they thus provide alternate discourses and agendas would be instructive.

  6. Social media and mobile communications adoption patterns of South African civil society organisations

    Directory of Open Access Journals (Sweden)

    Kiru Pillay

    2014-08-01

    Full Text Available Background: The resurgence of civil society has largely been attributed to the sector’s ability to exploit new interactive technologies and its ability to adapt its communication and mobilisation strategies. Objectives: This study focuses on how South African civil society organisations (CSOs deploy Web 2.0 services and technologies for social advocacy and the context of this technology use. Whilst the literature points to many studies relating to the use of the Internet for advocacy, it also suggests that the role and impact of emerging technologies have not been studied in any detail in CSOs. Such studies have the potential to provide new perspectives to current theoretical frameworks and also to add to the discourse around the use of emerging technologies for advocacy. Method: A survey of South African CSOs explored the level of knowledge of social media services and revealed which services in particular were being adopted. Results: The key findings that emerged were that the sector has a low level of knowledge of social media services and an accompanying low level of adoption. These are partly explained by factors such as macro-economic policies and low levels of Internet penetration and ICT readiness. Conclusion: Further research to determine why certain social media services have been embraced more willingly than others and an analysis of the patterns of adoption to determine any underlying significance or relationships is necessary. An analysis of how CSOs build their advocacy capabilities by appropriating social media and how they thus provide alternate discourses and agendas would be instructive.

  7. Development of allele-specific therapeutic siRNA in Meesmann epithelial corneal dystrophy.

    Directory of Open Access Journals (Sweden)

    Haihui Liao

    Full Text Available BACKGROUND: Meesmann epithelial corneal dystrophy (MECD is an inherited eye disorder caused by dominant-negative mutations in either keratins K3 or K12, leading to mechanical fragility of the anterior corneal epithelium, the outermost covering of the eye. Typically, patients suffer from lifelong irritation of the eye and/or photophobia but rarely lose visual acuity; however, some individuals are severely affected, with corneal scarring requiring transplant surgery. At present no treatment exists which addresses the underlying pathology of corneal dystrophy. The aim of this study was to design and assess the efficacy and potency of an allele-specific siRNA approach as a future treatment for MECD. METHODS AND FINDINGS: We studied a family with a consistently severe phenotype where all affected persons were shown to carry heterozygous missense mutation Leu132Pro in the KRT12 gene. Using a cell-culture assay of keratin filament formation, mutation Leu132Pro was shown to be significantly more disruptive than the most common mutation, Arg135Thr, which is associated with typical, mild MECD. A siRNA sequence walk identified a number of potent inhibitors for the mutant allele, which had no appreciable effect on wild-type K12. The most specific and potent inhibitors were shown to completely block mutant K12 protein expression with negligible effect on wild-type K12 or other closely related keratins. Cells transfected with wild-type K12-EGFP construct show a predominantly normal keratin filament formation with only 5% aggregate formation, while transfection with mutant K12-EGFP construct resulted in a significantly higher percentage of keratin aggregates (41.75%; p<0.001 with 95% confidence limits. The lead siRNA inhibitor significantly rescued the ability to form keratin filaments (74.75% of the cells contained normal keratin filaments; p<0.001 with 95% confidence limits. CONCLUSIONS: This study demonstrates that it is feasible to design highly potent si

  8. Older adults adopted more cautious gait patterns when walking in socks than barefoot.

    Science.gov (United States)

    Tsai, Yi-Ju; Lin, Sang-I

    2013-01-01

    Walking barefoot or in socks is common for ambulating indoors and has been reported to be associated with increased risk of falls and related injuries in the elderly. This study sought to determine if gait patterns differed between these two conditions for young and older adults. A motion analysis system was used to record and calculate the stride characteristics and motion of the body's center of mass (COM) of 21 young and 20 older adults. For the walking tasks, the participants walked on a smooth floor surface at their preferred speed either barefoot or in socks in a random order. The socks were commercially available and commonly used. The results demonstrated that while walking in socks, compared with walking barefoot, older adults adopted a more cautious gait pattern including decreased walking speed and shortened stride length as well as reduced COM minimal velocity during the single limb support phase. Young adults, however, did not demonstrate significant changes. These findings suggest that walking with socks might present a greater balance threat for older adults. Clinically, safety precautions about walking in socks should be considered to be given to older adults, especially those with balance deficits.

  9. Efficient CRISPR-rAAV engineering of endogenous genes to study protein function by allele-specific RNAi.

    Science.gov (United States)

    Kaulich, Manuel; Lee, Yeon J; Lönn, Peter; Springer, Aaron D; Meade, Bryan R; Dowdy, Steven F

    2015-04-20

    Gene knockout strategies, RNAi and rescue experiments are all employed to study mammalian gene function. However, the disadvantages of these approaches include: loss of function adaptation, reduced viability and gene overexpression that rarely matches endogenous levels. Here, we developed an endogenous gene knockdown/rescue strategy that combines RNAi selectivity with a highly efficient CRISPR directed recombinant Adeno-Associated Virus (rAAV) mediated gene targeting approach to introduce allele-specific mutations plus an allele-selective siRNA Sensitive (siSN) site that allows for studying gene mutations while maintaining endogenous expression and regulation of the gene of interest. CRISPR/Cas9 plus rAAV targeted gene-replacement and introduction of allele-specific RNAi sensitivity mutations in the CDK2 and CDK1 genes resulted in a >85% site-specific recombination of Neo-resistant clones versus ∼8% for rAAV alone. RNAi knockdown of wild type (WT) Cdk2 with siWT in heterozygotic knockin cells resulted in the mutant Cdk2 phenotype cell cycle arrest, whereas allele specific knockdown of mutant CDK2 with siSN resulted in a wild type phenotype. Together, these observations demonstrate the ability of CRISPR plus rAAV to efficiently recombine a genomic locus and tag it with a selective siRNA sequence that allows for allele-selective phenotypic assays of the gene of interest while it remains expressed and regulated under endogenous control mechanisms.

  10. SNPsplit: Allele-specific splitting of alignments between genomes with known SNP genotypes [version 1; referees: 2 approved

    Directory of Open Access Journals (Sweden)

    Felix Krueger

    2016-06-01

    Full Text Available Sequencing reads overlapping polymorphic sites in diploid mammalian genomes may be assigned to one allele or the other. This holds the potential to detect gene expression, chromatin modifications, DNA methylation or nuclear interactions in an allele-specific fashion. SNPsplit is an allele-specific alignment sorter designed to read files in SAM/BAM format and determine the allelic origin of reads or read-pairs that cover known single nucleotide polymorphic (SNP positions. For this to work libraries must have been aligned to a genome in which all known SNP positions were masked with the ambiguity base ’N’ and aligned using a suitable mapping program such as Bowtie2, TopHat, STAR, HISAT2, HiCUP or Bismark. SNPsplit also provides an automated solution to generate N-masked reference genomes for hybrid mouse strains based on the variant call information provided by the Mouse Genomes Project. The unique ability of SNPsplit to work with various different kinds of sequencing data including RNA-Seq, ChIP-Seq, Bisulfite-Seq or Hi-C opens new avenues for the integrative exploration of allele-specific data.

  11. SNPsplit: Allele-specific splitting of alignments between genomes with known SNP genotypes [version 2; referees: 3 approved

    Directory of Open Access Journals (Sweden)

    Felix Krueger

    2016-07-01

    Full Text Available Sequencing reads overlapping polymorphic sites in diploid mammalian genomes may be assigned to one allele or the other. This holds the potential to detect gene expression, chromatin modifications, DNA methylation or nuclear interactions in an allele-specific fashion. SNPsplit is an allele-specific alignment sorter designed to read files in SAM/BAM format and determine the allelic origin of reads or read-pairs that cover known single nucleotide polymorphic (SNP positions. For this to work libraries must have been aligned to a genome in which all known SNP positions were masked with the ambiguity base 'N' and aligned using a suitable mapping program such as Bowtie2, TopHat, STAR, HISAT2, HiCUP or Bismark. SNPsplit also provides an automated solution to generate N-masked reference genomes for hybrid mouse strains based on the variant call information provided by the Mouse Genomes Project. The unique ability of SNPsplit to work with various different kinds of sequencing data including RNA-Seq, ChIP-Seq, Bisulfite-Seq or Hi-C opens new avenues for the integrative exploration of allele-specific data.

  12. Allele-specific enzymatic amplification of beta-globin genomic DNA for diagnosis of sickle cell anemia.

    Science.gov (United States)

    Wu, D Y; Ugozzoli, L; Pal, B K; Wallace, R B

    1989-04-01

    A rapid nonradioactive approach to the diagnosis of sickle cell anemia is described based on an allele-specific polymerase chain reaction (ASPCR). This method allows direct detection of the normal or the sickle cell beta-globin allele in genomic DNA without additional steps of probe hybridization, ligation, or restriction enzyme cleavage. Two allele-specific oligonucleotide primers, one specific for the sickle cell allele and one specific for the normal allele, together with another primer complementary to both alleles were used in the polymerase chain reaction with genomic DNA templates. The allele-specific primers differed from each other in their terminal 3' nucleotide. Under the proper annealing temperature and polymerase chain reaction conditions, these primers only directed amplification on their complementary allele. In a single blind study of DNA samples from 12 individuals, this method correctly and unambiguously allowed for the determination of the genotypes with no false negatives or positives. If ASPCR is able to discriminate all allelic variation (both transition and transversion mutations), this method has the potential to be a powerful approach for genetic disease diagnosis, carrier screening, HLA typing, human gene mapping, forensics, and paternity testing.

  13. A Singular Vision for a Disparate Future: Technology Adoption Patterns in Higher Learning through 2035

    Science.gov (United States)

    Henshaw, Robert G.

    2008-01-01

    Technology adoption in any sector is rarely uniform. Understanding the drivers and constraints associated with technology adoption makes it easier to anticipate how technology will be used and what populations will benefit the most. Robert G. Henshaw examines factors likely to influence technology adoption within U.S. higher education over the…

  14. A single tube modified allele-specific-PCR for rapid detection of erythromycin-resistant Mycoplasma pneumoniae in Beijing

    Institute of Scientific and Technical Information of China (English)

    LI Shao-li; SUN Hong-mei; ZHAO Han-qing; CAO Ling; YUAN Yi; FENG Yan-ling; XUE Guan-hua

    2012-01-01

    Background Mycoplasma pneumoniae (M.pneumoniae) is one of the common pathogens causing atypical pneumonia.In recent years,resistance to macrolides has become more common,especially in China.Previous studies have confirmed that the mutation at position 2063 in domain V of the 23S rRNA is the most prevalent,followed by the mutation at position 2064.Reported molecular detection methods for the identification of these mutations include direct sequencing,restriction fragment length polymorphism analysis,real-time polymerase chain reaction (PCR) with high-resolution melt analysis,and nested PCR-linked with capillary electrophoresis,etc.The most commonly used method for monitoring resistance-conferring mutations in M.pneumoniae is direct DNA sequencing of PCR or nested PCR products.However,these methods are time-consuming,labor-intensive or need expensive equipments.Therefore the development of rapid and sensitive methods is very important for monitoring the resistance globally.Methods In this study,we reported a fast and cost-effective method for detecting 2063 and/or 2064 macrolide resistant mutations from specimens using a modified allele-specific PCR analysis,and all results were compared with the sequencing data.We also analyzed the clinical courses of these samples to confirm the modified allele-specific PCR results.Results Among 97 M.pneumoniae specimens,88 were found to possess mutations by this method,and all modified allele-specific PCR analysis results were consistent with the sequencing data.The data of the clinical courses of these 97cases showed that they suffered from severe pneumonia.Erythromycin showed better efficacy on cases from which no macrolide resistance mutation was found on their specimens.However,in some cases from which mutations were detected,erythromycin monotherapy had poor efficacy,and on these patients severe symptoms improved only when azithromycin was added to the treatment.Conclusions The drug-resistant M.pneumoniae is very common in

  15. Agencification and marketization in Danish and Swedish state administration - patterns of adoption

    DEFF Research Database (Denmark)

    Hansen, Morten Balle; Niklasson, Birgitta

    We analyze similarities and differences in the adoption of marketization and agencification practices in state administration in the two Nordic countries: Denmark and Sweden. Based on a literature review and on contrasting notions of a global management reform movement enforcing more similarity (...... to have adopted significantly more practices related to marketization than their Swedish equivalents. We discuss the possible interpretation of our findings.......We analyze similarities and differences in the adoption of marketization and agencification practices in state administration in the two Nordic countries: Denmark and Sweden. Based on a literature review and on contrasting notions of a global management reform movement enforcing more similarity...

  16. Polymorphism analysis of Chinese Theileria sergenti using allele-specific polymerase chain reaction of the major piroplasm surface protein gene.

    Science.gov (United States)

    Liu, Ai Hong; Guan, Gui Quan; Liu, Jun Long; Liu, Zhi Jie; Leblanc, Neil; Li, You Quan; Gao, Jin Liang; Ma, Mi Ling; Niu, Qing Li; Ren, Qiao Yun; Bai, Qi; Yin, Hong; Luo, Jian Xun

    2011-02-01

    Theileria sergenti is a tick-borne parasite found in many parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a marker for epidemiological and phylogenetic studies of benign Theileria species. In this study, Chinese species of T. sergenti were characterized by allele-specific polymerase chain reaction (PCR) and DNA sequence analysis of the MPSP gene. Using universal or allele-specific primer sets for PCR amplification of the MPSP gene, 98 of 288 cattle blood samples, collected from 6 provinces in China, were found to be positive. Among the positive samples, only 3 allelic MPSP gene types (Chitose [C]-, Ikeda [I]-, and buffeli [B]-type) were successfully amplified. Moreover, the results revealed that the majority of the parasites sampled in this study were C- and I-type (prevalence of 84 and 69%, respectively), whereas the B-type was less common (prevalence of 36%). Co-infections with C-, I-, and B-type T. sergenti also were found. An additional known allele, Thai-type, was not detected. Phylogenetic analysis based on the MPSP gene sequences, including 3 standard stocks generated in the laboratory ( T. sergenti Wenchuan, T. sergenti Ningxian, and T. sergenti Liaoyang), revealed that the isolates of Chinese sergenti were comprised of at least 4 allelic MPSP gene types, i.e., C-, I-, B1-, and B2-type, and these parasites with 6 MPSP types 1-5 and 7 were present in China.

  17. Use of allele-specific FAIRE to determine functional regulatory polymorphism using large-scale genotyping arrays

    DEFF Research Database (Denmark)

    Smith, Frank Andrew; Howard, Philip; Shah, Sonia;

    2012-01-01

    identified an allele-specific regulatory polymorphism within NR1H3 (coding for LXR-α), rs7120118, coinciding with a previously GWAS-identified SNP for HDL-C levels. This finding was confirmed using FAIRE-gen with the 200,000 SNP Illumina Metabochip and verified with the established method of TaqMan allelic...... variants, we describe the technique of allele-specific FAIRE, utilising large-scale genotyping technology (FAIRE-gen) to determine allelic effects on chromatin accessibility and regulatory potential. FAIRE-gen was explored using lymphoblastoid cells and the 50,000 SNP Illumina CVD BeadChip. The technique...... discrimination. Examination of this SNP in two prospective Caucasian cohorts comprising 15,000 individuals confirmed the association with HDL-C levels (combined beta = 0.016; p = 0.0006), and analysis of gene expression identified an allelic association with LXR-α expression in heart tissue. Using increasingly...

  18. Ploidy mosaicism and allele-specific gene expression differences in the allopolyploid Squalius alburnoides

    Directory of Open Access Journals (Sweden)

    Matos Isa

    2011-12-01

    Full Text Available Abstract Background Squalius alburnoides is an Iberian cyprinid fish resulting from an interspecific hybridisation between Squalius pyrenaicus females (P genome and males of an unknown Anaecypris hispanica-like species (A genome. S. alburnoides is an allopolyploid hybridogenetic complex, which makes it a likely candidate for ploidy mosaicism occurrence, and is also an interesting model to address questions about gene expression regulation and genomic interactions. Indeed, it was previously suggested that in S. alburnoides triploids (PAA composition silencing of one of the three alleles (mainly of the P allele occurs. However, not a whole haplome is inactivated but a more or less random inactivation of alleles varying between individuals and even between organs of the same fish was seen. In this work we intended to correlate expression differences between individuals and/or between organs to the occurrence of mosaicism, evaluating if mosaics could explain previous observations and its impact on the assessment of gene expression patterns. Results To achieve our goal, we developed flow cytometry and cell sorting protocols for this system generating more homogenous cellular and transcriptional samples. With this set-up we detected 10% ploidy mosaicism within the S. alburnoides complex, and determined the allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin in cells from liver and kidney of mosaic and non-mosaic individuals coming from different rivers over a wide geographic range. Conclusions Ploidy mosaicism occurs sporadically within the S. alburnoides complex, but in a frequency significantly higher than reported for other organisms. Moreover, we could exclude the influence of this phenomenon on the detection of variable allelic expression profiles of ubiquitously expressed genes (rpl8; gapdh and β-actin in cells from liver and kidney of triploid individuals. Finally, we determined that the expression patterns

  19. Understanding the technology of computer technology diffusion: explaining computer adoption patterns and implications for the wage structure

    OpenAIRE

    Borghans, L.; ter Weel, B.J.

    2008-01-01

    We review the empirical literature about the implications of the computerization of the labor market to see whether it can explain observed computer adoption patterns and (long-term) changes in the wage structure. Evidence from empirical micro-economic studies turns out to be inconsistent with macro-economic studies that are based on Constant Elasticity of Substitution (CES) production functions. We propose a micro-economic foundation for the CES production function that allows for changes in...

  20. An allele-specific polymerase chain reaction assay for the differentiation of members of the Anopheles culicifacies complex

    Indian Academy of Sciences (India)

    O P Singh; Geeta Goswami; N Nanda; K Raghavendra; D Chandra; S K Subbarao

    2004-09-01

    Anopheles culicifacies, the principal vector of malaria in India, is a complex of five cryptic species which are morphologically indistinguishable at any stage of life. In view of the practical difficulties associated with classical cytotaxonomic method for the identification of members of the complex, an allele-specific polymerase chain reaction (ASPCR) assay targeted to the D3 domain of 28S ribosomal DNA was developed. The assay discriminates An. culicifacies species A and D from species B, C and E. The assay was validated using chromosomally-identified specimens of An. culicifacies from different geographical regions of India representing different sympatric associations. The assay correctly differentiates species A and D from species B, C and E. The possible use of this diagnostic assay in disease vector control programmes is discussed.

  1. A Novel Real-time Fluorescence Mutant-allele-specific Amplification Method for Rapid Single Nucleotide Polymorphism Analysis

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Current methods for single nucleotide polymorphism (SNP) analysis are timeconsuming and complicated. We aimed at development of one-step real-time fluorescence mutant-allele-specific amplification (MASA) method for rapid SNP analysis. The method is a marriage of two technologies: MASA primers for target DNA and a double-stranded DNA-selective fluorescent dye, SYBR Green I. Genotypes are separated according to the different threshold cycles of the wild-type and mutant primers. K-ras oncogene was used as a target to validate the feasibility of the method. The experimental results showed that the different genotypes can be clearly discriminated by the assay. The real-time fluorescence MASA method will have an enormous potential for fast and reliable SNP analysis due to its simplicity and low cost.

  2. Powerful identification of cis-regulatory SNPs in human primary monocytes using allele-specific gene expression.

    Directory of Open Access Journals (Sweden)

    Jonas Carlsson Almlöf

    Full Text Available A large number of genome-wide association studies have been performed during the past five years to identify associations between SNPs and human complex diseases and traits. The assignment of a functional role for the identified disease-associated SNP is not straight-forward. Genome-wide expression quantitative trait locus (eQTL analysis is frequently used as the initial step to define a function while allele-specific gene expression (ASE analysis has not yet gained a wide-spread use in disease mapping studies. We compared the power to identify cis-acting regulatory SNPs (cis-rSNPs by genome-wide allele-specific gene expression (ASE analysis with that of traditional expression quantitative trait locus (eQTL mapping. Our study included 395 healthy blood donors for whom global gene expression profiles in circulating monocytes were determined by Illumina BeadArrays. ASE was assessed in a subset of these monocytes from 188 donors by quantitative genotyping of mRNA using a genome-wide panel of SNP markers. The performance of the two methods for detecting cis-rSNPs was evaluated by comparing associations between SNP genotypes and gene expression levels in sample sets of varying size. We found that up to 8-fold more samples are required for eQTL mapping to reach the same statistical power as that obtained by ASE analysis for the same rSNPs. The performance of ASE is insensitive to SNPs with low minor allele frequencies and detects a larger number of significantly associated rSNPs using the same sample size as eQTL mapping. An unequivocal conclusion from our comparison is that ASE analysis is more sensitive for detecting cis-rSNPs than standard eQTL mapping. Our study shows the potential of ASE mapping in tissue samples and primary cells which are difficult to obtain in large numbers.

  3. Assignment of SNP allelic configuration in polyploids using competitive allele-specific PCR: application to citrus triploid progeny

    Science.gov (United States)

    Cuenca, José; Aleza, Pablo; Navarro, Luis; Ollitrault, Patrick

    2013-01-01

    Background Polyploidy is a major component of eukaryote evolution. Estimation of allele copy numbers for molecular markers has long been considered a challenge for polyploid species, while this process is essential for most genetic research. With the increasing availability and whole-genome coverage of single nucleotide polymorphism (SNP) markers, it is essential to implement a versatile SNP genotyping method to assign allelic configuration efficiently in polyploids. Scope This work evaluates the usefulness of the KASPar method, based on competitive allele-specific PCR, for the assignment of SNP allelic configuration. Citrus was chosen as a model because of its economic importance, the ongoing worldwide polyploidy manipulation projects for cultivar and rootstock breeding, and the increasing availability of SNP markers. Conclusions Fifteen SNP markers were successfully designed that produced clear allele signals that were in agreement with previous genotyping results at the diploid level. The analysis of DNA mixes between two haploid lines (Clementine and pummelo) at 13 different ratios revealed a very high correlation (average = 0·9796; s.d. = 0·0094) between the allele ratio and two parameters [θ angle = tan−1 (y/x) and y′ = y/(x + y)] derived from the two normalized allele signals (x and y) provided by KASPar. Separated cluster analysis and analysis of variance (ANOVA) from mixed DNA simulating triploid and tetraploid hybrids provided 99·71 % correct allelic configuration. Moreover, triploid populations arising from 2n gametes and interploid crosses were easily genotyped and provided useful genetic information. This work demonstrates that the KASPar SNP genotyping technique is an efficient way to assign heterozygous allelic configurations within polyploid populations. This method is accurate, simple and cost-effective. Moreover, it may be useful for quantitative studies, such as relative allele-specific expression analysis and bulk segregant analysis

  4. Plantar fibromatosis may adopt the brain gyriform pattern of a low-grade fibromyxoid sarcoma.

    Science.gov (United States)

    Touraine, Sébastien; Bousson, Valérie; Kaci, Rachid; Parlier-Cuau, Caroline; Haddad, Samuel; Laouisset, Liess; Petrover, David; Laredo, Jean-Denis

    2013-01-01

    We report the case of a 42-year-old man with histologically proven plantar fibromatosis (Ledderhose disease) demonstrating an uncommon brain gyriform pattern at MRI, so far exclusively described in the low-grade fibromyxoid sarcoma (LGFMS). An acoustic posterior enhancement at ultrasound, a high intensity on T2w and post-contrast T1wMR images were unusual and related to a high tumor cellularity at histology with no myxoid tissue. The juxtaposition of areas of high and low cellularity (with more fibrous material) in a multilobulated mass built a brain gyriform pattern at MR, similar to what was so far described exclusively in LGFMS. This case demonstrates that the brain gyriform pattern may also be observed in other soft tissue fibrous tumors with no myxoid material but with high cellularity areas alternating with fibrous zones of low cellularity.

  5. Adoption of Agro-forestry Patterns and Crop Systems Around Register 19 Forest Park, Lampung Province, Indonesia

    Directory of Open Access Journals (Sweden)

    Christine Wulandari

    2014-09-01

    Full Text Available To return the ecological function of Wan Abdul Rachman Forest Park, it must be involved the role of buffer zone communities living around the forest by optimizing the cultivated land with applying agro-forestry based on socio-economic conditions in the community, such as community preferences and adoption of agro-forestry patterns. Under these conditions it is necessary to hold a study concerning to the level of community preference to the type of plants and the level of adoption, as well as the NPV analysis of the 3 patterns of agro-forestry which are applied by the majority of community around the forest park. Results of the study revealed that there were 3 dominant plant types  preferred by the community in the forest park, namely: coffee (30.8%, cacao (35.8%, and rubber (17.4%. Based on these crops, there were 3 agro-forestry patterns practiced by the majority of community in their cultivated land. They were: (1 coffee-cacao-wooden plants, and fruits (47%, (2 rubber-coffee-wooden plants, and fruit (35%, and (3 rubber-cacao-wooden plants, and fruit (18%. The highest personal and social NPVs obtained  in the agro-forestry pattern of rubber-coffee-wooded plants, and fruits were IDR4.589.627.36 and IDR6.454.806.01, respectively. To ensure the sustainability of the program, the development of communities living around the forest together with a program of community empowerment in the block of utilization and social forestry in the forest park are recommended to continue, based on the Regional Regulation (PerDa Number. 3/2012.Keywords: agro-forestry, preferences, adoption, NPV

  6. Allele-specific suppression of mutant huntingtin using antisense oligonucleotides: providing a therapeutic option for all Huntington disease patients.

    Directory of Open Access Journals (Sweden)

    Niels H Skotte

    Full Text Available Huntington disease (HD is an inherited, fatal neurodegenerative disorder caused by a CAG repeat expansion in the huntingtin gene. The mutant protein causes neuronal dysfunction and degeneration resulting in motor dysfunction, cognitive decline, and psychiatric disturbances. Currently, there is no disease altering treatment, and symptomatic therapy has limited benefit. The pathogenesis of HD is complicated and multiple pathways are compromised. Addressing the problem at its genetic root by suppressing mutant huntingtin expression is a promising therapeutic strategy for HD. We have developed and evaluated antisense oligonucleotides (ASOs targeting single nucleotide polymorphisms that are significantly enriched on HD alleles (HD-SNPs. We describe our structure-activity relationship studies for ASO design and find that adjusting the SNP position within the gap, chemical modifications of the wings, and shortening the unmodified gap are critical for potent, specific, and well tolerated silencing of mutant huntingtin. Finally, we show that using two distinct ASO drugs targeting the two allelic variants of an HD-SNP could provide a therapeutic option for all persons with HD; allele-specifically for roughly half, and non-specifically for the remainder.

  7. Ribosomal protein genes are highly enriched among genes with allele-specific expression in the interspecific F1 hybrid catfish.

    Science.gov (United States)

    Chen, Ailu; Wang, Ruijia; Liu, Shikai; Peatman, Eric; Sun, Luyang; Bao, Lisui; Jiang, Chen; Li, Chao; Li, Yun; Zeng, Qifan; Liu, Zhanjiang

    2016-06-01

    Interspecific hybrids provide a rich source for the analysis of allele-specific expression (ASE). In this work, we analyzed ASE in F1 hybrid catfish using RNA-Seq datasets. While the vast majority of genes were expressed with both alleles, 7-8 % SNPs exhibited significant differences in allele ratios of expression. Of the 66,251 and 177,841 SNPs identified from the datasets of the liver and gill, 5420 (8.2 %) and 13,390 (7.5 %) SNPs were identified as significant ASE-SNPs, respectively. With these SNPs, a total of 1519 and 3075 ASE-genes were identified. Gene Ontology analysis revealed that genes encoding cytoplasmic ribosomal proteins (RP) were highly enriched among ASE genes. Parent-of-origin was determined for 27 and 30 ASE RP genes in the liver and gill, respectively. The results indicated that genes from both channel catfish and blue catfish were involved in ASE. However, each RP gene appeared to be almost exclusively expressed from only one parent, indicating that ribosomes in the hybrid catfish were in the "hybrid" form. Overall representation of RP transcripts among the transcriptome appeared lower in the F1 hybrid catfish than in channel catfish or blue catfish, suggesting that the "hybrid" ribosomes may work more efficiently for translation in the F1 hybrid catfish.

  8. Adoption of Agro-forestry Patterns and Crop Systems Around Register 19 Forest Park, Lampung Province, Indonesia

    Directory of Open Access Journals (Sweden)

    Christine Wulandari

    2014-08-01

    Full Text Available To return the ecological function of Wan Abdul Rachman Forest Park, it must be involved the role of buffer zone communities living around the forest by optimizing the cultivated land with applying agro-forestry based on socio-economic conditions in the community, such as community preferences and adoption of agro-forestry patterns. Under these conditions it is necessary to hold a study concerning to the level of community preference to the type of plants and the level of adoption, as well as the NPV analysis of the 3 patterns of agro-forestry which are applied by the majority of community around the forest park. Results of the study revealed that there were 3 dominant plant types preferred by the community in the forest park, namely: coffee (30.8%, cacao (35.8%, and rubber (17.4%. Based on these crops, there were 3 agro-forestry patterns practiced by the majority of community in their cultivated land. They were: (1 coffee-cacao-wooden plants, and fruits (47%, (2 rubber-coffee-wooden plants, and fruit (35%, and (3 rubber-cacao-wooden plants, and fruit (18%. The highest personal and social NPVs obtained in the agro-forestry pattern of rubber-coffee-wooded plants, and fruits were IDR4.589.627.36 and IDR6.454.806.01, respectively. To ensure the sustainability of the program, the development of communities living around the forest together with a program of community empowerment in the block of utilization and social forestry in the forest park are recommended to continue, based on the Regional Regulation (PerDa Number. 3/2012.

  9. Quantitative polymerase chain reaction analysis with allele-specific oligonucleotide primers for individual IgH VDJ regions to evaluate tumor burden in myeloma patients.

    Science.gov (United States)

    Sata, Hiroshi; Shibayama, Hirohiko; Maeda, Ikuhiro; Habuchi, Yoko; Nakatani, Eiji; Fukushima, Kentaro; Fujita, Jiro; Ezoe, Sachiko; Tadokoro, Seiji; Maeda, Tetsuo; Mizuki, Masao; Kosugi, Satoru; Nakagawa, Masashi; Ueda, Shuji; Iida, Masato; Tokumine, Yukihiro; Azenishi, Yasuhiko; Mitsui, Hideki; Oritani, Kenji; Kanakura, Yuzuru

    2015-05-01

    Quantitative polymerase chain reaction (PCR) with patient-specific, allele-specific oligonucleotide (ASO) primers for individual immunoglobulin H VDJ region (ASO-PCR) amplification was performed using several sources of clinical material, including mRNA from peripheral blood cells (PBMNCs), whole bone marrow cells (BMMNCs), and the CD20+ CD38- B-cell population in bone marrow, as well as cell-free DNA from the sera of patients with multiple myeloma (MM). We designed the ASO primers and produced sufficient PCR fragments to evaluate tumor burden in 20 of 30 bone marrow samples at diagnosis. Polymerase chain reaction amplification efficiency depended on primer sequences because the production of ASO-PCR fragments did not correlate with serum M-protein levels. However, the ASO-PCR levels in BMMNCs showed statistically significant correlations with those in PBMNCs and CD20+ CD38- B-cells. The good association between the BMMNC and PBMNC data indicated that PBMNCs could be a suitable source for monitoring minimal residual disease (MRD). In the case of cell-free DNA, ASO-PCR levels showed a unique pattern and remained high even after treatment. Because the sequence information for each ASO-PCR product was identical to the original, the cell-free DNA might also be useful for evaluating MRD. Moreover, the ASO-PCR products were clearly detected in 17 of 22 mRNA samples from CD20+ CD38- populations, suggesting that MM clones might exist in relatively earlier stages of B cells than in plasma cells. Thus, ASO-PCR analysis using various clinical materials is useful for detecting MRD in MM patients as well as for clarifying MM pathogenesis.

  10. Integrative analysis of hereditary nonpolyposis colorectal cancer: the contribution of allele-specific expression and other assays to diagnostic algorithms.

    Directory of Open Access Journals (Sweden)

    Laura De Lellis

    Full Text Available The identification of germline variants predisposing to hereditary nonpolyposis colorectal cancer (HNPCC is crucial for clinical management of carriers, but several probands remain negative for such variants or bear variants of uncertain significance (VUS. Here we describe the results of integrative molecular analyses in 132 HNPCC patients providing evidences for improved genetic testing of HNPCC with traditional or next generation methods. Patients were screened for: germline allele-specific expression (ASE, nucleotide variants, rearrangements and promoter methylation of mismatch repair (MMR genes; germline EPCAM rearrangements; tumor microsatellite instability (MSI and immunohistochemical (IHC MMR protein expression. Probands negative for pathogenic variants of MMR genes were screened for germline APC and MUTYH sequence variants. Most germline defects identified were sequence variants and rearrangements of MMR genes. Remarkably, altered germline ASE of MMR genes was detected in 8/22 (36.5% probands analyzed, including 3 cases negative at other screenings. Moreover, ASE provided evidence for the pathogenic role and guided the characterization of a VUS shared by 2 additional probands. No germline MMR gene promoter methylation was observed and only one EPCAM rearrangement was detected. In several cases, tumor IHC and MSI diverged from germline screening results. Notably, APC or biallelic MUTYH germline defects were identified in 2/19 probands negative for pathogenic variants of MMR genes. Our results show that ASE complements gDNA-based analyses in the identification of MMR defects and in the characterization of VUS affecting gene expression, increasing the number of germline alterations detected. An appreciable fraction of probands negative for MMR gene variants harbors APC or MUTYH variants. These results indicate that germline ASE analysis and screening for APC and MUTYH defects should be included in HNPCC diagnostic algorithms.

  11. Oncogene mutations, copy number gains and mutant allele specific imbalance (MASI frequently occur together in tumor cells.

    Directory of Open Access Journals (Sweden)

    Junichi Soh

    Full Text Available BACKGROUND: Activating mutations in one allele of an oncogene (heterozygous mutations are widely believed to be sufficient for tumorigenesis. However, mutant allele specific imbalance (MASI has been observed in tumors and cell lines harboring mutations of oncogenes. METHODOLOGY/PRINCIPAL FINDINGS: We determined 1 mutational status, 2 copy number gains (CNGs and 3 relative ratio between mutant and wild type alleles of KRAS, BRAF, PIK3CA and EGFR genes by direct sequencing and quantitative PCR assay in over 400 human tumors, cell lines, and xenografts of lung, colorectal, and pancreatic cancers. Examination of a public database indicated that homozygous mutations of five oncogenes were frequent (20% in 833 cell lines of 12 tumor types. Our data indicated two major forms of MASI: 1 MASI with CNG, either complete or partial; and 2 MASI without CNG (uniparental disomy; UPD, due to complete loss of wild type allele. MASI was a frequent event in mutant EGFR (75% and was due mainly to CNGs, while MASI, also frequent in mutant KRAS (58%, was mainly due to UPD. Mutant: wild type allelic ratios at the genomic level were precisely maintained after transcription. KRAS mutations or CNGs were significantly associated with increased ras GTPase activity, as measured by ELISA, and the two molecular changes were synergistic. Of 237 lung adenocarcinoma tumors, the small number with both KRAS mutation and CNG were associated with shortened survival. CONCLUSIONS: MASI is frequently present in mutant EGFR and KRAS tumor cells, and is associated with increased mutant allele transcription and gene activity. The frequent finding of mutations, CNGs and MASI occurring together in tumor cells indicates that these three genetic alterations, acting together, may have a greater role in the development or maintenance of the malignant phenotype than any individual alteration.

  12. Infrequent detection of germline allele-specific expression of TGFBR1 in lymphoblasts and tissues of colon cancer patients.

    LENUS (Irish Health Repository)

    Guda, Kishore

    2009-06-15

    Recently, germline allele-specific expression (ASE) of the gene encoding for transforming growth factor-beta type I receptor (TGFBR1) has been proposed to be a major risk factor for cancer predisposition in the colon. Germline ASE results in a lowered expression of one of the TGFBR1 alleles (>1.5-fold), and was shown to occur in approximately 20% of informative familial and sporadic colorectal cancer (CRC) cases. In the present study, using the highly quantitative pyrosequencing technique, we estimated the frequency of ASE in TGFBR1 in a cohort of affected individuals from familial clusters of advanced colon neoplasias (cancers and adenomas with high-grade dysplasia), and also from a cohort of individuals with sporadic CRCs. Cases were considered positive for the presence of ASE if demonstrating an allelic expression ratio <0.67 or >1.5. Using RNA derived from lymphoblastoid cell lines, we find that of 46 informative Caucasian advanced colon neoplasia cases with a family history, only 2 individuals display a modest ASE, with allelic ratios of 1.65 and 1.73, respectively. Given that ASE of TGFBR1, if present, would likely be more pronounced in the colon compared with other tissues, we additionally determined the allele ratios of TGFBR1 in the RNA derived from normal-appearing colonic mucosa of sporadic CRC cases. We, however, found no evidence of ASE in any of 44 informative sporadic cases analyzed. Taken together, we find that germline ASE of TGFBR1, as assayed in lymphoblastoid and colon epithelial cells of colon cancer patients, is a relatively rare event.

  13. Identification of transcriptome SNPs between Xiphophorus lines and species for assessing allele specific gene expression within F1 interspecies hybrids☆

    Science.gov (United States)

    Shen, Yingjia; Catchen, Julian; Garcia, Tzintzuni; Amores, Angel; Beldroth, Ion; Wagner, Jonathon R; Zhang, Ziping; Postlethwait, John; Warren, Wes; Schartl, Manfred; Walter, Ronald B.

    2011-01-01

    Variations in gene expression are essential for the evolution of novel phenotypes and for speciation. Studying allelic specific gene expression (ASGE) within interspecies hybrids provides a unique opportunity to reveal underlying mechanisms of genetic variation. Using Xiphophorus interspecies hybrid fishes and high-throughput next generation sequencing technology, we were able to assess variations between two closely related vertebrate species, X. maculatus and X. couchianus, and their F1 interspecies hybrids. We constructed transcriptome-wide SNP polymorphism sets between two highly inbred X. maculatus lines (JP 163 A and B), and between X. maculatus and a second species, X. couchianus. The X. maculatus JP 163 A and B parental lines have been separated in the laboratory for ≈ 70 years and we were able to identify SNPs at a resolution of 1 SNP per 49 kb of transcriptome. In contrast, SNP polymorphisms between X. couchianus and X. maculatus species, which diverged ≈ 5–10 million years ago, were identified about every 700 bp. Using 6,524 transcripts with identified SNPs between the two parental species (X. maculatus and X. couchianus), we mapped RNA-seq reads to determine ASGE within F1 interspecies hybrids. We developed an in silico X. couchianus transcriptome by replacing 90,788 SNP bases for X. maculatus transcriptome with the consensus X. couchianus SNP bases and provide evidence that this procedure overcomes read mapping biases. Employment of the insilico reference transcriptome and tolerating 5 mismatches during read mapping allow direct assessment of ASGE in the F1 interspecies hybrids. Overall, these results show that Xiphophorus is a tractable vertebrate experimental model to investigate how genetic variations that occur during speciation may affect gene interactions and the regulation of gene expression. PMID:21466860

  14. Identification of transcriptome SNPs between Xiphophorus lines and species for assessing allele specific gene expression within F₁ interspecies hybrids.

    Science.gov (United States)

    Shen, Yingjia; Catchen, Julian; Garcia, Tzintzuni; Amores, Angel; Beldorth, Ion; Wagner, Jonathan; Zhang, Ziping; Postlethwait, John; Warren, Wes; Schartl, Manfred; Walter, Ronald B

    2012-01-01

    Variations in gene expression are essential for the evolution of novel phenotypes and for speciation. Studying allelic specific gene expression (ASGE) within interspecies hybrids provides a unique opportunity to reveal underlying mechanisms of genetic variation. Using Xiphophorus interspecies hybrid fishes and high-throughput next generation sequencing technology, we were able to assess variations between two closely related vertebrate species, Xiphophorus maculatus and Xiphophorus couchianus, and their F(1) interspecies hybrids. We constructed transcriptome-wide SNP polymorphism sets between two highly inbred X. maculatus lines (JP 163 A and B), and between X. maculatus and a second species, X. couchianus. The X. maculatus JP 163 A and B parental lines have been separated in the laboratory for ≈70 years and we were able to identify SNPs at a resolution of 1 SNP per 49 kb of transcriptome. In contrast, SNP polymorphisms between X. couchianus and X. maculatus species, which diverged ≈5-10 million years ago, were identified about every 700 bp. Using 6524 transcripts with identified SNPs between the two parental species (X. maculatus and X. couchianus), we mapped RNA-seq reads to determine ASGE within F(1) interspecies hybrids. We developed an in silico X. couchianus transcriptome by replacing 90,788 SNP bases for X. maculatus transcriptome with the consensus X. couchianus SNP bases and provide evidence that this procedure overcomes read mapping biases. Employment of the in silico reference transcriptome and tolerating 5 mismatches during read mapping allow direct assessment of ASGE in the F(1) interspecies hybrids. Overall, these results show that Xiphophorus is a tractable vertebrate experimental model to investigate how genetic variations that occur during speciation may affect gene interactions and the regulation of gene expression.

  15. Detection of Fusarium oxysporum f. sp. vasinfectum race 3 by single-base extension method and allele-specific polymerase chain reaction

    Science.gov (United States)

    We developed allele specific (AS) SNP primers for rapid detection of Fusarium oxysporum f.sp vasinfectum (FOV) race 3. FOV_BT_SNP_R3 and FOV_BT_AS_R3 primers were designed based on single nucleotide polymorphisms of partial sequence alignment of the ß-tubulin (BT) gene from several FOV races. These ...

  16. Identifying breast cancer risk loci by global differential allele-specific expression (DASE analysis in mammary epithelial transcriptome

    Directory of Open Access Journals (Sweden)

    Gao Chuan

    2012-10-01

    Full Text Available Abstract Background The significant mortality associated with breast cancer (BCa suggests a need to improve current research strategies to identify new genes that predispose women to breast cancer. Differential allele-specific expression (DASE has been shown to contribute to phenotypic variables in humans and recently to the pathogenesis of cancer. We previously reported that nonsense-mediated mRNA decay (NMD could lead to DASE of BRCA1/2, which is associated with elevated susceptibility to breast cancer. In addition to truncation mutations, multiple genetic and epigenetic factors can contribute to DASE, and we propose that DASE is a functional index for cis-acting regulatory variants and pathogenic mutations, and that global analysis of DASE in breast cancer precursor tissues can be used to identify novel causative alleles for breast cancer susceptibility. Results To test our hypothesis, we employed the Illumina® Omni1-Quad BeadChip in paired genomic DNA (gDNA and double-stranded cDNA (ds-cDNA samples prepared from eight BCa patient-derived normal mammary epithelial lines (HMEC. We filtered original array data according to heterozygous genotype calls and calculated DASE values using the Log ratio of cDNA allele intensity, which was normalized to the corresponding gDNA. We developed two statistical methods, SNP- and gene-based approaches, which allowed us to identify a list of 60 candidate DASE loci (DASE ≥ 2.00, P ≤ 0.01, FDR ≤ 0.05 by both methods. Ingenuity Pathway Analysis of DASE loci revealed one major breast cancer-relevant interaction network, which includes two known cancer causative genes, ZNF331 (DASE = 2.31, P = 0.0018, FDR = 0.040 and USP6 (DASE = 4.80, P = 0.0013, FDR = 0.013, and a breast cancer causative gene, DMBT1 (DASE=2.03, P = 0.0017, FDR = 0.014. Sequence analysis of a 5′ RACE product of DMBT1 demonstrated that rs2981745, a putative breast cancer risk locus, appears to be one of the causal variants leading to DASE

  17. WASP: a Web-based Allele-Specific PCR assay designing tool for detecting SNPs and mutations

    Directory of Open Access Journals (Sweden)

    Assawamakin Anunchai

    2007-08-01

    Full Text Available Abstract Background Allele-specific (AS Polymerase Chain Reaction is a convenient and inexpensive method for genotyping Single Nucleotide Polymorphisms (SNPs and mutations. It is applied in many recent studies including population genetics, molecular genetics and pharmacogenomics. Using known AS primer design tools to create primers leads to cumbersome process to inexperience users since information about SNP/mutation must be acquired from public databases prior to the design. Furthermore, most of these tools do not offer the mismatch enhancement to designed primers. The available web applications do not provide user-friendly graphical input interface and intuitive visualization of their primer results. Results This work presents a web-based AS primer design application called WASP. This tool can efficiently design AS primers for human SNPs as well as mutations. To assist scientists with collecting necessary information about target polymorphisms, this tool provides a local SNP database containing over 10 million SNPs of various populations from public domain databases, namely NCBI dbSNP, HapMap and JSNP respectively. This database is tightly integrated with the tool so that users can perform the design for existing SNPs without going off the site. To guarantee specificity of AS primers, the proposed system incorporates a primer specificity enhancement technique widely used in experiment protocol. In particular, WASP makes use of different destabilizing effects by introducing one deliberate 'mismatch' at the penultimate (second to last of the 3'-end base of AS primers to improve the resulting AS primers. Furthermore, WASP offers graphical user interface through scalable vector graphic (SVG draw that allow users to select SNPs and graphically visualize designed primers and their conditions. Conclusion WASP offers a tool for designing AS primers for both SNPs and mutations. By integrating the database for known SNPs (using gene ID or rs number

  18. Impact of pre-existing MSP142-allele specific immunity on potency of an erythrocytic Plasmodium falciparum vaccine

    Directory of Open Access Journals (Sweden)

    Bergmann-Leitner Elke S

    2012-09-01

    Full Text Available Abstract Background MSP1 is the major surface protein on merozoites and a prime candidate for a blood stage malaria vaccine. Preclinical and seroepidemiological studies have implicated antibodies to MSP1 in protection against blood stage parasitaemia and/or reduced parasite densities, respectively. Malaria endemic areas have multiple strains of Plasmodium falciparum circulating at any given time, giving rise to complex immune responses, an issue which is generally not addressed in clinical trials conducted in non-endemic areas. A lack of understanding of the effect of pre-existing immunity to heterologous parasite strains may significantly contribute to vaccine failure in the field. The purpose of this study was to model the effect of pre-existing immunity to MSP142 on the immunogenicity of blood-stage malaria vaccines based on alternative MSP1 alleles. Methods Inbred and outbred mice were immunized with various recombinant P. falciparum MSP142 proteins that represent the two major alleles of MSP142, MAD20 (3D7 and Wellcome (K1, FVO. Humoral immune responses were analysed by ELISA and LuminexTM, and functional activity of induced MSP142-specific antibodies was assessed by growth inhibition assays. T-cell responses were characterized using ex vivo ELISpot assays. Results Analysis of the immune responses induced by various immunization regimens demonstrated a strong allele-specific response at the T cell level in both inbred and outbred mice. The success of heterologous regimens depended on the degree of homology of the N-terminal p33 portion of the MSP142, likely due to the fact that most T cell epitopes reside in this part of the molecule. Analysis of humoral immune responses revealed a marked cross-reactivity between the alleles. Functional analyses showed that some of the heterologous regimens induced antibodies with improved growth inhibitory activities. Conclusion The development of a more broadly efficacious MSP1 based vaccine may be

  19. Allele-specific PCR for detecting the deafness-associated mitochondrial 12S rRNA mutations.

    Science.gov (United States)

    Ding, Yu; Xia, Bo-Hou; Liu, Qi; Li, Mei-Ya; Huang, Shui-Xian; Zhuo, Guang-Chao

    2016-10-10

    Mutations in mitochondrial 12S rRNA (MT-RNR1) are the important causes of sensorineural hearing loss. Of these mutations, the homoplasmic m.1555A>G or m.1494C>T mutation in the highly conserved A-site of MT-RNR1 gene has been found to be associated with both aminoglycoside-induced and non-syndromic hearing loss in many families worldwide. Since the m.1555A>G and m.1494C>T mutations are sensitive to ototoxic drugs, therefore, screening for the presence of these mutations is important for early diagnosis and prevention of deafness. For this purpose, we recently developed a novel allele-specific PCR (AS-PCR) which is able to simultaneously detect these mutations. To assess its accuracy, in this study, we employed this method to screen the frequency of m.1555A>G and m.1494C>T mutations in 200 deafness patients and 120 healthy subjects. Consequently, four m.1555A>G and four m.1494C>T mutations were identified; among these, only one patient with the m.1494C>T mutation had an obvious family history of hearing loss. Strikingly, clinical evaluation showed that this family exhibited a high penetrance of hearing loss. In particular, the penetrances of hearing loss were 80% with the aminoglycoside included and 20% when excluded. PCR-Sanger sequencing of the mitochondrial genomes confirmed the presence of the m.1494C>T mutation and identified a set of polymorphisms belonging to mitochondrial haplogroup A. However, the lack of functional variants in mitochondrial and nuclear modified genes (GJB2 and TRMU) in this family indicated that mitochondrial haplogroup and nuclear genes may not play important roles in the phenotypic expression of the m.1494C>T mutation. Thus, other modification factors, such as environmental factor, aminoglycosides or epigenetic modification may have contributed to the high penetrance of hearing loss in this family. Taken together, our data showed that this assay is an effective approach that could be used for detection the deafness-associated MT-RNR1

  20. Computer Attitude, and the Impact of Personal Characteristics and Information and Communication Technology Adoption Patterns on Performance of Teaching Faculty in Higher Education in Ghana, West Africa

    Science.gov (United States)

    Larbi-Apau, Josephine A.

    2011-01-01

    This study examined computer attitude, and the impact of personal characteristics and ICT adoption patterns on performance of multidisciplinary teaching faculty in three public universities in Ghana. A cross-sectional research of mixed methods was applied in collecting data and information. Quantitative data from 164 respondents were analyzed…

  1. Allele-specific chromatin remodeling in the ZPBP2/GSDMB/ORMDL3 locus associated with the risk of asthma and autoimmune disease.

    Science.gov (United States)

    Verlaan, Dominique J; Berlivet, Soizik; Hunninghake, Gary M; Madore, Anne-Marie; Larivière, Mathieu; Moussette, Sanny; Grundberg, Elin; Kwan, Tony; Ouimet, Manon; Ge, Bing; Hoberman, Rose; Swiatek, Marcin; Dias, Joana; Lam, Kevin C L; Koka, Vonda; Harmsen, Eef; Soto-Quiros, Manuel; Avila, Lydiana; Celedón, Juan C; Weiss, Scott T; Dewar, Ken; Sinnett, Daniel; Laprise, Catherine; Raby, Benjamin A; Pastinen, Tomi; Naumova, Anna K

    2009-09-01

    Common SNPs in the chromosome 17q12-q21 region alter the risk for asthma, type 1 diabetes, primary biliary cirrhosis, and Crohn disease. Previous reports by us and others have linked the disease-associated genetic variants with changes in expression of GSDMB and ORMDL3 transcripts in human lymphoblastoid cell lines (LCLs). The variants also alter regulation of other transcripts, and this domain-wide cis-regulatory effect suggests a mechanism involving long-range chromatin interactions. Here, we further dissect the disease-linked haplotype and identify putative causal DNA variants via a combination of genetic and functional analyses. First, high-throughput resequencing of the region and genotyping of potential candidate variants were performed. Next, additional mapping of allelic expression differences in Yoruba HapMap LCLs allowed us to fine-map the basis of the cis-regulatory differences to a handful of candidate functional variants. Functional assays identified allele-specific differences in nucleosome distribution, an allele-specific association with the insulator protein CTCF, as well as a weak promoter activity for rs12936231. Overall, this study shows a common disease allele linked to changes in CTCF binding and nucleosome occupancy leading to altered domain-wide cis-regulation. Finally, a strong association between asthma and cis-regulatory haplotypes was observed in three independent family-based cohorts (p = 1.78 x 10(-8)). This study demonstrates the requirement of multiple parallel allele-specific tools for the investigation of noncoding disease variants and functional fine-mapping of human disease-associated haplotypes.

  2. Allele-specific polymerase chain reaction for detection of a mutation in the relax circular DNA and the covalently closed circular DNA of hepatitis B virus.

    Science.gov (United States)

    Pan, Wan-Long; Hu, Jie-Li; Fang, Yan; Luo, Qiang; Xu, Ge; Xu, Lei; Jing, Zhou-Hong; Shan, Xue-Feng; Zhu, Yan-Ling; Huang, Ai-Long

    2013-12-01

    The relax circle DNA (rcDNA) sequence and the covalently closed circle DNA (cccDNA) sequence in hepatitis B virus (HBV) are crucial regions for HBV infections. To analyze mutations in rcDNA and cccDNA, DNA sequencing is often used, although it is time-consuming and expensive. Herein, we report a simple, economic, albeit accurate allele-specific polymerase chain reaction (AS-PCR) to detect mutations in these regions of HBV. This method can be extensively used to screen for mutations at specific positions of HBV genome.

  3. Simultaneous genotyping of single-nucleotide polymorphisms in alcoholism-related genes using duplex and triplex allele-specific PCR with two-step thermal cycles.

    Science.gov (United States)

    Shirasu, Naoto; Kuroki, Masahide

    2014-01-01

    We developed a time- and cost-effective multiplex allele-specific polymerase chain reaction (AS-PCR) method based on the two-step PCR thermal cycles for genotyping single-nucleotide polymorphisms in three alcoholism-related genes: alcohol dehydrogenase 1B, aldehyde dehydrogenase 2 and μ-opioid receptor. Applying MightyAmp(®) DNA polymerase with optimized AS-primers and PCR conditions enabled us to achieve effective and selective amplification of the target alleles from alkaline lysates of a human hair root, and simultaneously to determine the genotypes within less than 1.5 h using minimal lab equipment.

  4. New prediction model for probe specificity in an allele-specific extension reaction for haplotype-specific extraction (HSE) of Y chromosome mixtures.

    Science.gov (United States)

    Rothe, Jessica; Watkins, Norman E; Nagy, Marion

    2012-01-01

    Allele-specific extension reactions (ASERs) use 3' terminus-specific primers for the selective extension of completely annealed matches by polymerase. The ability of the polymerase to extend non-specific 3' terminal mismatches leads to a failure of the reaction, a process that is only partly understood and predictable, and often requires time-consuming assay design. In our studies we investigated haplotype-specific extraction (HSE) for the separation of male DNA mixtures. HSE is an ASER and provides the ability to distinguish between diploid chromosomes from one or more individuals. Here, we show that the success of HSE and allele-specific extension depend strongly on the concentration difference between complete match and 3' terminal mismatch. Using the oligonucleotide-modeling platform Visual Omp, we demonstrated the dependency of the discrimination power of the polymerase on match- and mismatch-target hybridization between different probe lengths. Therefore, the probe specificity in HSE could be predicted by performing a relative comparison of different probe designs with their simulated differences between the duplex concentration of target-probe match and mismatches. We tested this new model for probe design in more than 300 HSE reactions with 137 different probes and obtained an accordance of 88%.

  5. Enhanced specificity of TPMT*2 genotyping using unidirectional wild-type and mutant allele-specific scorpion primers in a single tube.

    Science.gov (United States)

    Chen, Dong; Yang, Zhao; Xia, Han; Huang, Jun-Fu; Zhang, Yang; Jiang, Tian-Nun; Wang, Gui-Yu; Chuai, Zheng-Ran; Fu, Wei-Ling; Huang, Qing

    2014-01-01

    Genotyping of thiopurine S-methyltransferase (TPMT) is recommended for predicting the adverse drug response of thiopurines. In the current study, a novel version of allele-specific PCR (AS-PCR), termed competitive real-time fluorescent AS-PCR (CRAS-PCR) was developed to analyze the TPMT*2 genotype in ethnic Chinese. This technique simultaneously uses wild-type and mutant allele-specific scorpion primers in a single reaction. To determine the optimal conditions for both traditional AS-PCR and CRAS-PCR, we used the Taguchi method, an engineering optimization process that balances the concentrations of all components using an orthogonal array rather than a factorial array. Instead of running up to 264 experiments with the conventional factorial method, the Taguchi method achieved the same optimization using only 16 experiments. The optimized CRAS-PCR system completely avoided non-specific amplification occurring in traditional AS-PCR and could be performed at much more relaxed reaction conditions at 1% sensitivity, similar to traditional AS-PCR. TPMT*2 genotyping of 240 clinical samples was consistent with published data. In conclusion, CRAS-PCR is a novel and robust genotyping method, and the Taguchi method is an effective tool for the optimization of molecular analysis techniques.

  6. Allele Specific Locked Nucleic Acid Quantitative PCR (ASLNAqPCR): An Accurate and Cost-Effective Assay to Diagnose and Quantify KRAS and BRAF Mutation

    Science.gov (United States)

    Morandi, Luca; de Biase, Dario; Visani, Michela; Cesari, Valentina; De Maglio, Giovanna; Pizzolitto, Stefano; Pession, Annalisa; Tallini, Giovanni

    2012-01-01

    The use of tyrosine kinase inhibitors (TKIs) requires the testing for hot spot mutations of the molecular effectors downstream the membrane-bound tyrosine kinases since their wild type status is expected for response to TKI therapy. We report a novel assay that we have called Allele Specific Locked Nucleic Acid quantitative PCR (ASLNAqPCR). The assay uses LNA-modified allele specific primers and LNA-modified beacon probes to increase sensitivity, specificity and to accurately quantify mutations. We designed primers specific for codon 12/13 KRAS mutations and BRAF V600E, and validated the assay with 300 routine samples from a variety of sources, including cytology specimens. All were analyzed by ASLNAqPCR and Sanger sequencing. Discordant cases were pyrosequenced. ASLNAqPCR correctly identified BRAF and KRAS mutations in all discordant cases and all had a mutated/wild type DNA ratio below the analytical sensitivity of the Sanger method. ASLNAqPCR was 100% specific with greater accuracy, positive and negative predictive values compared with Sanger sequencing. The analytical sensitivity of ASLNAqPCR is 0.1%, allowing quantification of mutated DNA in small neoplastic cell clones. ASLNAqPCR can be performed in any laboratory with real-time PCR equipment, is very cost-effective and can easily be adapted to detect hot spot mutations in other oncogenes. PMID:22558339

  7. The Plasmodium falciparum merozoite surface protein-1 19 KD antibody response in the Peruvian Amazon predominantly targets the non-allele specific, shared sites of this antigen

    Directory of Open Access Journals (Sweden)

    Silva Claudia

    2010-01-01

    Full Text Available Abstract Background Plasmodium falciparum re-emerged in Iquitos, Peru in 1994 and is now hypoendemic (P. falciparum infections can be followed using this population dynamic. Previous work demonstrated a strong association between this population's antibody response to PfMSP1-19KD and protection against febrile illness and parasitaemia. Therefore, some selection for PfMSP1-19KD allelic diversity would be expected if the protection is to allele-specific sites of PfMSP1-19KD. Here, the potential for allele-specific polymorphisms in this population is investigated, and the allele-specificity of antibody responses to PfMSP1-19KD are determined. Methods The 42KD region in PfMSP1 was genotyped from 160 individual infections collected between 2003 and 2007. Additionally, the polymorphic block 2 region of Pfmsp1 (Pfmsp1-B2 was genotyped in 781 infection-months to provide a baseline for population-level diversity. To test whether PfMSP1-19KD genetic diversity had any impact on antibody responses, ELISAs testing IgG antibody response were performed on individuals using all four allele-types of PfMSP1-19KD. An antibody depletion ELISA was used to test the ability of antibodies to cross-react between allele-types. Results Despite increased diversity in Pfmsp1-B2, limited diversity within Pfmsp1-42KD was observed. All 160 infections genotyped were Mad20-like at the Pfmsp1-33KD locus. In the Pfmsp1-19KD locus, 159 (99.4% were the Q-KSNG-F haplotype and 1 (0.6% was the E-KSNG-L haplotype. Antibody responses in 105 individuals showed that Q-KNG and Q-TSR alleles generated the strongest immune responses, while Q-KNG and E-KNG responses were more concordant with each other than with those from Q-TSR and E-TSR, and vice versa. The immuno-depletion ELISAs showed all samples responded to the antigenic sites shared amongst all allelic forms of PfMSP1-19KD. Conclusions A non-allele specific antibody response in PfMSP1-19KD may explain why other allelic forms have not

  8. Detection of EGFR mutations in plasma and biopsies from non-small cell lung cancer patients by allele-specific PCR assays

    DEFF Research Database (Denmark)

    Weber, Britta; Meldgaard, Peter; Hager, Henrik

    2014-01-01

    BACKGROUND: Lung cancer patients with mutations in the epidermal growth factor receptor (EGFR) are primary candidates for EGFR-targeted therapy. Reliable analyses of such mutations have previously been possible only in tumour tissue. Here, we demonstrate that mutations can be detected in plasma...... samples with allele-specific PCR assays. METHODS: Pairs of the diagnostic biopsy and plasma obtained just prior to start of erlotinib treatment were collected from 199 patients with adenocarcinoma of non-small-cell lung cancer. DNA from both sample types was isolated and examined for the presence...... identified in 24/199 (12%) plasma samples and 28/196 (14%) biopsy samples, and 17/196 (9%) matched pairs contained the same mutation. Six EGFR mutations were present only in plasma samples but not in the biopsy samples. The overall concordance of the EGFR gene mutations detected in plasma and biopsy tissue...

  9. Allele-specific real-time PCR testing for minor HIV-1 drug resistance mutations: assay preparation and application to reveal dynamic of mutations in vivo

    Institute of Scientific and Technical Information of China (English)

    GUO Dong-xing; LI Jing-yun; LI Han-ping; LI Lin; ZHUANG Dao-min; JIAO Li-yan; WANG Zheng; BAO Zuo-yi; LIU Si-yang; LIU Yong-jian

    2010-01-01

    Background It is very important for the clinical management to test for minor HIV-1 resistance mutations accurately and sensitively. The conventional genotypic assays of HIV drug resistance detection based on sequencing can only discriminate the mutations which present in more than 20%-30%. The aim of this study was to evaluate allele-specific real-time PCR (ASPCR) to detect the resistance-related mutations located at positions 103, 184 and 215.Methods We developed the allele-specific PCR assay, using the most common drug resistance mutations in Chinese AIDS patients, K103N, M184V/I, T215F/Y as a model system. The standards were constructed by cloning the wild-type and mutant DNA fragments into the T-vector. We designed specific primers to discriminate mutant templates in the real-time PCR using SYBR green as a fluorescence reporter. And then we evaluated the ASPCR assay and tested 140clinical samples using this method.Results The sensitivities of ASPCR assay were 0.04% for K103N, 0.30% for M1841, 0.40% for M184V, 0.03% for T215F and 0.02% for T215Y. The intra-assay and inter-assay coefficients of variation were less than 0.42. One hundred and forty plasma samples were tested by ASPCR and dynamic resistance curves of ten patients were obtained.Conclusions Drug resistance emerged half a year after the start of antiretroviral therapy. The mutation of T215Yemerged 1 to 1.5 years after starting treatment and then increased rapidly. The ASPCR assay we developed was a sensitive, accurate and rapid method to detect the minor HIV-1 variants and it can provide earlier and more drug-resistance information for HIV research and AIDS antiretroviral therapy.

  10. Disagreement in genotyping results of drug resistance alleles of the Plasmodium falciparum dihydrofolate reductase (Pfdhfr) gene by allele-specific PCR (ASPCR) assays and Sanger sequencing.

    Science.gov (United States)

    Sharma, Divya; Lather, Manila; Dykes, Cherry L; Dang, Amita S; Adak, Tridibes; Singh, Om P

    2016-01-01

    The rapid spread of antimalarial drug resistance in Plasmodium falciparum over the past few decades has necessitated intensive monitoring of such resistance for an effective malaria control strategy. P. falciparum dihydropteroate synthase (Pfdhps) and P. falciparum dihydrofolate reductase (Pfdhfr) genes act as molecular markers for resistance against the antimalarial drugs sulphadoxine and pyrimethamine, respectively. Resistance to pyrimethamine which is used as a partner drug in artemisinin combination therapy (ACT) is associated with several mutations in the Pfdhfr gene, namely A16V, N51I, C59R, S108N/T and I164L. Therefore, routine monitoring of Pfdhfr-drug-resistant alleles in a population may help in effective drug resistance management. Allele-specific PCR (ASPCR) is one of the commonly used methods for molecular genotyping of these alleles. In this study, we genotyped 55 samples of P. falciparum for allele discrimination at four codons of Pfdhfr (N51, C59, S108 and I164) by ASPCR using published methods and by Sanger's DNA sequencing method. We found that the ASPCR identified a significantly higher number of mutant alleles as compared to the DNA sequencing method. Such discrepancies arise due to the non-specificity of some of the allele-specific primer sets and due to the lack of sensitivity of Sanger's DNA sequencing method to detect minor alleles present in multiple clone infections. This study reveals the need of a highly specific and sensitive method for genotyping and detecting minor drug-resistant alleles present in multiple clonal infections.

  11. Enhancement of allele discrimination by introduction of nucleotide mismatches into siRNA in allele-specific gene silencing by RNAi.

    Directory of Open Access Journals (Sweden)

    Yusuke Ohnishi

    Full Text Available Allele-specific gene silencing by RNA interference (RNAi is therapeutically useful for specifically inhibiting the expression of disease-associated alleles without suppressing the expression of corresponding wild-type alleles. To realize such allele-specific RNAi (ASP-RNAi, the design and assessment of small interfering RNA (siRNA duplexes conferring ASP-RNAi is vital; however, it is also difficult. In a previous study, we developed an assay system to assess ASP-RNAi with mutant and wild-type reporter alleles encoding the Photinus and Renilla luciferase genes. In line with experiments using the system, we realized that it is necessary and important to enhance allele discrimination between mutant and corresponding wild-type alleles. Here, we describe the improvement of ASP-RNAi against mutant alleles carrying single nucleotide variations by introducing base substitutions into siRNA sequences, where original variations are present in the central position. Artificially mismatched siRNAs or short-hairpin RNAs (shRNAs against mutant alleles of the human Prion Protein (PRNP gene, which appear to be associated with susceptibility to prion diseases, were examined using this assessment system. The data indicates that introduction of a one-base mismatch into the siRNAs and shRNAs was able to enhance discrimination between the mutant and wild-type alleles. Interestingly, the introduced mismatches that conferred marked improvement in ASP-RNAi, appeared to be largely present in the guide siRNA elements, corresponding to the 'seed region' of microRNAs. Due to the essential role of the 'seed region' of microRNAs in their association with target RNAs, it is conceivable that disruption of the base-pairing interactions in the corresponding seed region, as well as the central position (involved in cleavage of target RNAs, of guide siRNA elements could influence allele discrimination. In addition, we also suggest that nucleotide mismatches at the 3'-ends of sense

  12. 云计算中的云采用模式%Cloud Adoption Patterns of Cloud Computing

    Institute of Scientific and Technical Information of China (English)

    蔡仲博

    2014-01-01

    Model for complex tasks contains the best practices and expertise,it is helpful to clearly define the format of a good solution to deal with recurring problems. Plans to implement a cloud rendering companies should be"cloud adoption model"is considered a way to start the process. This article describes how to use the cloud model to support enterprises using cloud to achieve business and technical requirements. By scenes showing how cloud solutions provider to build private and public cloud, the client application is hosted in a cloud environment all or part of hosting in the cloud, part hosted locally.%模式包含适用于复杂任务的最佳实践和专业知识,有助于以清晰的格式定义良好的解决方案,以便处理反复出现的问题。计划实现一个云呈现的企业应该将“云采用模式”视为启动该流程的一种方法。介绍了如何应用云采用模式来支持企业云实现的业务和技术需求,通过场景展示云解决方案。讨论了供应商如何构建私有和公共云,给出了客户端将应用程序全部托管在云环境中或者部分托管在云中,或者部分托管在本地。

  13. Adjustment of Daily Activities: the Influence of Smartphone Adoption on the Travel Pattern of Mobile Professionals in the Greater Jakarta Area

    Directory of Open Access Journals (Sweden)

    Gloriani Novita Christin

    2014-12-01

    Full Text Available The swift augmentation in the adoption of smartphones, the gadget that resulted from the convergence of Information and Communication Technology (ICT, potentially transforms people's life in myriad dimensions. One potential change induced by smartphones, is how people restructure their daily agenda and consecutively influence their travel pattern. To understand it, this study theoretically reviews mobile professional work, smartphone adoption, and how people conduct their mobile interaction, planning and execution of daily activities. Mobile professionals, the cohort of professionals that spend more than 20% of their total working time moving around out of their work environment; they are important beneficiaries of smartphones and have been chosen as the target of this study. Empirical results of mobile professionals´ experiences in the Greater Jakarta Area are presented at this juncture. Furthermore, their adjustment of activities as a dynamic response to receiving extensive information via smartphones is also analysed. The results indicate that there is a strong adjustment of daily activities by mobile professionals. Through those changes, the transformation of daily travel patterns related to the activity is also brought about by the use of this high-end ICT contrivance.

  14. A single-tube allele specific-polymerase chain reaction to detect T315I resistant mutation in chronic myeloid leukemia patients

    Directory of Open Access Journals (Sweden)

    Auewarakul Chirayu U

    2011-02-01

    Full Text Available Abstract Background BCR-ABL kinase domain (KD mutation is the major mechanism contributing to suboptimal response to tyrosine kinase inhibitors (TKI in BCR-ABL-positive chronic myeloid leukemia (CML patients. T315I mutation, as one of the most frequent KD mutations, has been shown to be strongly associated with TKI resistance and subsequent therapeutic failure. A simple and sensitive method is thus required to detect T315I mutation at the earliest stage. Methods A single-tube allele specific-polymerase chain reaction (AS-PCR method was developed to detect T315I mutation in a mixture of normal and mutant alleles of varying dilutions. Denaturing high performance liquid chromatography (DHPLC and direct sequencing were performed as a comparison to AS-PCR. Results T315I mutant bands were observed in the mixtures containing as low as 0.5-1% of mutant alleles by AS-PCR. The detection sensitivity of DHPLC was around 1.5-3% dilution whereas sequencing analysis was unable to detect below 6.25% dilution. Conclusion A single-tube AS-PCR is a rapid and sensitive screening method for T315I mutation. Detection of the most resistant leukemic clone in CML patients undergoing TKI therapy should be feasible with this simple and inexpensive method.

  15. A and MdMYB1 allele-specific markers controlling apple (Malus x domestica Borkh.) skin color and suitability for marker-assisted selection.

    Science.gov (United States)

    Zhang, X J; Wang, L X; Chen, X X; Liu, Y L; Meng, R; Wang, Y J; Zhao, Z Y

    2014-01-01

    Pre-selection for fruit skin color at the seedling stage would be highly advantageous, with marker-assisted selection offering a potential method for apple pre-selection. A and MdMYB1 alleles are allele-specific DNA markers that are potentially associated with apple skin color, and co-segregate with the Rf and Rni loci, respectively. Here, we assessed the potential application of these 2 alleles for marker-assisted breeding across 30 diverse cultivars and 2 apple seedling progenies. The red skin color phenotype was usually associated with the MdMYB1-1 allele and A(1) allele, respectively, while the 2 molecular markers provided approximately 91% predictability in the 'Fuji' x 'Cripps Pink' and 'Fuji' x 'Gala' progenies. The results obtained from the 30 cultivars and 2 progenies were consistent for the 2 molecular markers. Hence, the results supported that Rf and Rni could be located in a gene cluster, or even correspond to alleles of the same gene. Our results are consistent with the hypothesis that red/yellow dimorphism is controlled by a monogenic system, with the presence of the red anthocyanin pigmentation being dominant. In addition, our results supported that the practical utilization of the 2 function markers to efficiently and accurately select red-skinned apple cultivars in apple scion breeding programs.

  16. Concordance between allele-specific PCR and ultra-deep pyrosequencing for the detection of HIV-1 non-nucleoside reverse transcriptase inhibitor resistance mutations

    Science.gov (United States)

    Hunt, Gillian M; Morris, Lynn; Moorthy, Anitha; Coovadia, Ashraf; Abrams, Elaine J; Strehlau, Renate; Kuhn, Louise; Persaud, Deborah

    2014-01-01

    Recent advances in genotyping technologies have allowed for detection of HIV-1 drug resistance mutations present at low levels. The presence and percentage of Y181C and K103N drug-resistant variants in the blood of 105 subtype C HIV-infected infants who failed single-dose nevirapine prophylaxis for HIV transmission were compared using two highly sensitive genotyping methods, allele-specific PCR (AS-PCR) and ultra-deep pyrosequencing. Significant correlations in detection between both methods were found for both Y181C (correlation coefficients of 0.94 [95% CI 0.91-0.96]) and K103N (0.89 [95% CI 0.84 – 0.92]) mutations. The majority of discordant specimens (3/5 Y181C and 8/11 K103N) had wild-type variants when population sequencing was used, but mutant variants were detectable at very low levels (≤5%) with either assay. This difference is most likely due to stochastic variations in the appearance of mutant variants. Overall, both AS-PCR and ultra-deep pyrosequencing methods have proven to be sensitive and accurate, and may confidently be used where feasible. PMID:25034127

  17. Electromobility Shift Assay Reveals Evidence in Favor of Allele-Specific Binding of RUNX1 to the 5' Hypersensitive Site 4-Locus Control Region.

    Science.gov (United States)

    Dehghani, Hossein; Ghobakhloo, Sepideh; Neishabury, Maryam

    2016-08-01

    In our previous studies on the Iranian β-thalassemia (β-thal) patients, we identified an association between the severity of the β-thal phenotype and the polymorphic palindromic site at the 5' hypersensitive site 4-locus control region (5'HS4-LCR) of the β-globin gene cluster. Furthermore, a linkage disequilibrium was observed between this region and XmnI-HBG2 in the patient population. Based on this data, it was suggested that the well-recognized phenotype-ameliorating role assigned to positive XmnI could be associated with its linked elements in the LCR. To investigate the functional significance of polymorphisms at the 5'HS4-LCR, we studied its influence on binding of transcription factors. Web-based predictions of transcription factor binding revealed a binding site for runt-related transcription factor 1 (RUNX1), when the allele at the center of the palindrome (TGGGG(A/G)CCCCA) was A but not when it was G. Furthermore, electromobility shift assay (EMSA) presented evidence in support of allele-specific binding of RUNX1 to 5'HS4. Considering that RUNX1 is a well-known regulator of hematopoiesis, these preliminary data suggest the importance of further studies to confirm this interaction and consequently investigate its functional and phenotypical relevance. These studies could help us to understand the molecular mechanism behind the phenotype modifying role of the 5'HS4-LCR polymorphic palindromic region (rs16912979), which has been observed in previous studies.

  18. SAAS-CNV: A Joint Segmentation Approach on Aggregated and Allele Specific Signals for the Identification of Somatic Copy Number Alterations with Next-Generation Sequencing Data.

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    Zhongyang Zhang

    2015-11-01

    Full Text Available Cancer genomes exhibit profound somatic copy number alterations (SCNAs. Studying tumor SCNAs using massively parallel sequencing provides unprecedented resolution and meanwhile gives rise to new challenges in data analysis, complicated by tumor aneuploidy and heterogeneity as well as normal cell contamination. While the majority of read depth based methods utilize total sequencing depth alone for SCNA inference, the allele specific signals are undervalued. We proposed a joint segmentation and inference approach using both signals to meet some of the challenges. Our method consists of four major steps: 1 extracting read depth supporting reference and alternative alleles at each SNP/Indel locus and comparing the total read depth and alternative allele proportion between tumor and matched normal sample; 2 performing joint segmentation on the two signal dimensions; 3 correcting the copy number baseline from which the SCNA state is determined; 4 calling SCNA state for each segment based on both signal dimensions. The method is applicable to whole exome/genome sequencing (WES/WGS as well as SNP array data in a tumor-control study. We applied the method to a dataset containing no SCNAs to test the specificity, created by pairing sequencing replicates of a single HapMap sample as normal/tumor pairs, as well as a large-scale WGS dataset consisting of 88 liver tumors along with adjacent normal tissues. Compared with representative methods, our method demonstrated improved accuracy, scalability to large cancer studies, capability in handling both sequencing and SNP array data, and the potential to improve the estimation of tumor ploidy and purity.

  19. SAAS-CNV: A Joint Segmentation Approach on Aggregated and Allele Specific Signals for the Identification of Somatic Copy Number Alterations with Next-Generation Sequencing Data.

    Science.gov (United States)

    Zhang, Zhongyang; Hao, Ke

    2015-11-01

    Cancer genomes exhibit profound somatic copy number alterations (SCNAs). Studying tumor SCNAs using massively parallel sequencing provides unprecedented resolution and meanwhile gives rise to new challenges in data analysis, complicated by tumor aneuploidy and heterogeneity as well as normal cell contamination. While the majority of read depth based methods utilize total sequencing depth alone for SCNA inference, the allele specific signals are undervalued. We proposed a joint segmentation and inference approach using both signals to meet some of the challenges. Our method consists of four major steps: 1) extracting read depth supporting reference and alternative alleles at each SNP/Indel locus and comparing the total read depth and alternative allele proportion between tumor and matched normal sample; 2) performing joint segmentation on the two signal dimensions; 3) correcting the copy number baseline from which the SCNA state is determined; 4) calling SCNA state for each segment based on both signal dimensions. The method is applicable to whole exome/genome sequencing (WES/WGS) as well as SNP array data in a tumor-control study. We applied the method to a dataset containing no SCNAs to test the specificity, created by pairing sequencing replicates of a single HapMap sample as normal/tumor pairs, as well as a large-scale WGS dataset consisting of 88 liver tumors along with adjacent normal tissues. Compared with representative methods, our method demonstrated improved accuracy, scalability to large cancer studies, capability in handling both sequencing and SNP array data, and the potential to improve the estimation of tumor ploidy and purity.

  20. Genome-wide detection of allele specific copy number variation associated with insulin resistance in African Americans from the HyperGEN study.

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    Marguerite R Irvin

    Full Text Available African Americans have been understudied in genome wide association studies of diabetes and related traits. In the current study, we examined the joint association of single nucleotide polymorphisms (SNPs and copy number variants (CNVs with fasting insulin and an index of insulin resistance (HOMA-IR in the HyperGEN study, a family based study with proband ascertainment for hypertension. This analysis is restricted to 1,040 African Americans without diabetes. We generated allele specific CNV genotypes at 872,243 autosomal loci using Birdsuite, a freely available multi-stage program. Joint tests of association for SNPs and CNVs were performed using linear mixed models adjusting for covariates and familial relationships. Our results highlight SNPs associated with fasting insulin and HOMA-IR (rs6576507 and rs8026527, 3.7*10(-7≤P≤1.1*10(-5 near ATPase, class V, type 10A (ATP10A, and the L Type voltage dependent calcium channel (CACNA1D, rs1401492, P≤5.2*10(-6. ATP10A belongs to a family of aminophospholipid-transporting ATPases and has been associated with type 2 diabetes in mice. CACNA1D has been linked to pancreatic beta cell generation in mice. The two most significant copy variable markers (rs10277702 and rs361367; P<2.0*10(-4 were in the beta variable region of the T-cell receptor gene (TCRVB. Human and mouse TCR has been shown to mimic insulin and its receptor and could contribute to insulin resistance. Our findings differ from genome wide association studies of fasting insulin and other diabetes related traits in European populations, highlighting the continued need to investigate unique genetic influences for understudied populations such as African Americans.

  1. Allelic diversity of a beer haze active protein gene in cultivated and Tibetan wild barley and development of allelic specific markers.

    Science.gov (United States)

    Ye, Lingzhen; Dai, Fei; Qiu, Long; Sun, Dongfa; Zhang, Guoping

    2011-07-13

    The formation of haze is a serious quality problem in beer production. It has been shown that the use of silica elute (SE)-ve malt (absence of molecular weight (MW) ∼14000 Da) for brewing can improve haze stability in the resultant beer, and the protein was identified as a barley trypsin inhibitor of the chloroform/methanol type (BTI-CMe). The objectives of this study were to determine (1) the allelic diversity of the gene controlling BTI-CMe in cultivated and Tibetan wild barley and (2) allele-specific (AS) markers for screening SE protein type. A survey of 172 Tibetan annual wild barley accessions and 71 cultivated barley genotypes was conducted, and 104 wild accessions and 35 cultivated genotypes were identified as SE+ve and 68 wild accessions and 36 cultivated genotypes as SE-ve. The allelic diversity of the gene controlling BTI-CMe was investigated by cloning, alignment, and association analysis. It was found that there were significant differences between the SE+ve and SE-ve types in single-nucleotide polymorphisms at 234 (SNP(234)), SNP(313), and SNP(385.) Furthermore, two sets of AS markers were developed to screen SE protein type based on SNP(313). AS-PCR had results very similar to those obtained by immunoblot method. Mapping analysis showed that the gene controlling the MW∼14 kDa band was located on the short arm of chromosome 3H, at the position of marker BPB-0527 (33.302 cM) in the Franklin/Yerong DH population.

  2. Minority drug-resistant HIV-1 variants in treatment naive East-African and Caucasian patients detected by allele-specific real-time PCR.

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    Halime Ekici

    Full Text Available To assess the presence of two major non-nucleoside reverse transcriptase inhibitors (NNRTI drug resistance mutations (DRMs, Y181C and K103N, in minor viral quasispecies of treatment naïve HIV-1 infected East-African and Swedish patients by allele-specific polymerase chain reaction (AS-PCR.Treatment naïve adults (n=191 with three epidemiological backgrounds were included: 92 Ethiopians living in Ethiopia; 55 East-Africans who had migrated to Sweden; and 44 Caucasians living in Sweden. The pol gene was analysed by standard population sequencing and by AS-PCR for the detection of Y181C and K103N.The Y181C was detected in the minority quasispecies of six Ethiopians (6.5%, in two Caucasians (4.5%, and in one East-African (1.8%. The K103N was detected in one East- African (1.8%, by both methods. The proportion of mutants ranged from 0.25% to 17.5%. Additional DRMs were found in all three treatment naïve patient groups by population sequencing.Major NNRTI mutations can be found by AS-PCR in minor quasispecies of treatment naïve HIV-1 infected Ethiopians living in Ethiopia, in East-African and Caucasian patients living in Sweden in whom population sequencing reveal wild-type virus only. Surveys with standard sequencing are likely to underestimate transmitted drug resistance and the presence of resistant minor quasispecies in treatment naïve patients should be topic for future large scale studies.

  3. Effects of a dietary intervention promoting the adoption of a Mediterranean food pattern on fast-food consumption among healthy French-Canadian women.

    Science.gov (United States)

    Bédard, Alexandra; Goulet, Julie; Riverin, Mélissa; Lamarche, Benoît; Lemieux, Simone

    2010-12-01

    It is expected that a dietary intervention based on the traditional Mediterranean food pattern should be associated with a reduction in fast-food consumption but this has never been tested before. We assessed the impact of a 12-week dietary intervention, promoting the adoption of a Mediterranean food pattern, on fast-food consumption among seventy-one healthy women aged between 30 and 65 years. The dietary intervention consisted of two group sessions and seven individual sessions with a dietitian. To determine the Mediterranean dietary score (MedScore) and fast-food consumption, an FFQ was administered. During the 12-week intervention, the MedScore significantly increased (from 21.1 (SD 3.6) units at baseline to 28.6 (SD 4.4) units at week 12, P food consumption significantly decreased (from 51.7 (SD 46.4) g/d at baseline to 20.5 (SD 18.2) g/d at week 12, P food consumption to the most (r - 0.50, P food consumption changes, it was found that only the subgroup of women which increased the most their MedScore and decreased the most their fast-food consumption experienced a significant decrease in BMI (P food consumption among healthy women even if it was not a specific target of the intervention. Dietary strategies for increasing intake of healthy foods may be a useful approach for decreasing intake of less healthy foods.

  4. Allele-specific oligonucleotide polymerase chain reaction for the determination of Rh C/c and Rh E/e antigens in thalassaemic patients

    Science.gov (United States)

    Hojjati, Mohammad Taher; Einollahi, Nahid; Nabatchian, Fariba; Pourfathollah, Ali Akbar; Mahdavi, Mohammad Reza

    2011-01-01

    Background Thalassaemia is a genetic disease in which there is a relative or complete lack of alpha or beta globin chains. Patients with moderate to severe forms of thalassaemia need transfusions from the early years of life. Antibody production against blood group antigens may cause many problems in preparing compatible blood units for transfusion. The identification of definite blood group phenotypes by the haemagglutination method can be difficult because of the mixed population of red blood cells from the donor and recipient. Materials and methods Forty multiply transfused thalassaemic patients and ten healthy controls with no history of blood transfusion were enrolled in this study. Allele-specific oligonucleotide polymerase chain reaction (ASO-PCR) and haemagglutination methods were used to determine the presence of Rhesus (Rh) C, c, E and e antigens. Results In this study four primer sets were used for ASO-PCR amplification of RhC/c and RhE/e. Although PCR assays for RhC/c and RHE/e genotyping have been described previously, in this study we used a new condition for PCR by decreasing the annealing temperature from 63 °C to 58 °C in order to amplify all four genes in the same condition. In order to evaluate this single run molecular method, we used the haemagglutination test as the standard method and compared the results from the two methods. We found discrepancies between phenotype and genotype results among patients with beta thalassaemia, but complete agreement between phenotype and genotype in the control group. Conclusions The advantage of this new ASO-PCR method compared to a restriction fragment length polymorphism (RFLP) PCR method is that with the former all four genes can be amplified at the same time by PCR, and electrophoresis can be performed immediately to determine individual antigen profiles. The simplicity of the ASO-PCR method makes it suitable for routine use in medical centres and it is also cheaper than RFLP-PCR. Furthermore, as shown

  5. Comparison of 454 Ultra-Deep Sequencing and Allele-Specific Real-Time PCR with Regard to the Detection of Emerging Drug-Resistant Minor HIV-1 Variants after Antiretroviral Prophylaxis for Vertical Transmission.

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    Andrea Hauser

    Full Text Available Pregnant HIV-infected women were screened for the development of HIV-1 drug resistance after implementation of a triple-antiretroviral transmission prophylaxis as recommended by the WHO in 2006. The study offered the opportunity to compare amplicon-based 454 ultra-deep sequencing (UDS and allele-specific real-time PCR (ASPCR for the detection of drug-resistant minor variants in the HIV-1 reverse transcriptase (RT.Plasma samples from 34 Tanzanian women were previously analysed by ASPCR for key resistance mutations in the viral RT selected by AZT, 3TC, and NVP (K70R, K103N, Y181C, M184V, T215Y/F. In this study, the RT region of the same samples was investigated by amplicon-based UDS for resistance mutations using the 454 GS FLX System.Drug-resistant HIV-variants were identified in 69% (20/29 of women by UDS and in 45% (13/29 by ASPCR. The absolute number of resistance mutations identified by UDS was twice that identified by ASPCR (45 vs 24. By UDS 14 of 24 ASPCR-detected resistance mutations were identified at the same position. The overall concordance between UDS and ASPCR was 61.0% (25/41. The proportions of variants quantified by UDS were approximately 2-3 times lower than by ASPCR. Amplicon generation from samples with viral loads below 20,000 copies/ml failed more frequently by UDS compared to ASPCR (limit of detection = 650 copies/ml, resulting in missing or insufficient sequence coverage.Both methods can provide useful information about drug-resistant minor HIV-1 variants. ASPCR has a higher sensitivity than UDS, but is restricted to single resistance mutations. In contrast, UDS is limited by its requirement for high viral loads to achieve sufficient sequence coverage, but the sequence information reveals the complete resistance patterns within the genomic region analysed. Improvements to the UDS limit of detection are in progress, and UDS could then facilitate monitoring of drug-resistant minor variants in the HIV-1 quasispecies.

  6. 全血AS-PCR方法检测Wilson病ATP7B基因四个突变%Whole blood allele-specific PCR, a simple method to detect four ATP7B gene mutations in Wilson disease

    Institute of Scientific and Technical Information of China (English)

    孙玮; 管俊杰; 王进; 秦正红

    2014-01-01

    Objective To establish a simple method to detect four ATP7B gene mutations in Wilson disease using allele-specific PCR (AS-PCR) with whole blood polymerase chain reaction.Methods Four allele-specific PCR primers specific for the mutations(G2333T,C2850T,G2855A,G2975T) were designed,and PCR was optimized to screen the whole blood samples.The amplified gene products with mutation were separated with agarose gel electrophoresis to detect the pattern of point mutation and allele types.Exons 8,12 and 13 of the ATP7B gene were amplified with PCR,and the amplification products were sequenced to confirm the mutation.Results The detection of four ATP7B gene mutations by AS-PCR with whole blood was accomplished with 100% accuracy.In the 27 healthy subjects,the mutation rate of G2855A was 51.8%.No mutation was detected for G2333T,C2850T and G2975T.Among the 22 patients,11 were mutated for G2333T,C2850T or G2975T.The mutation rate was therefore 50%.Conclusion Our experiment has established an AS-PCR based method for detecting four ATP7B gene mutations using whole blood samples,which has provided a simple and effective means for the early diagnosis of Wilson disease.This method is rapid,convenient,accurate and economical for detecting point mutations of the ATP7B gene.%目的 研究肝豆状核变性ATP7B基因高频突变位点,探索全血等位基因特异性-PCR(allelespecific PCR,AS-PCR)技术在该基因4个常见突变检测中的应用.方法 针对ATP7B基因4个突变位点(G2333T、C2850T、G2855A、G2975T)设计等位基因特异性引物,应用高保真酶对人抗凝全血样本进行聚合酶链反应,扩增产物经琼脂糖凝胶电泳以判断待检样本有无基因突变及其等位基因型.PCR扩增人基因组A TP7B基因第8、12、13外显子,扩增产物直接进行基因序列测定.结果 全血AS-PCR法检测ATP7B基因4个基因突变,各位点检测结果与基因序列测定完全相符.27份健康对照血样分型结果G2333T、C2850T

  7. Adopting Children with Attachment Problems.

    Science.gov (United States)

    Hughes, Daniel A.

    1999-01-01

    Notes that attachment behavior in infants is a facet of normal child development, and that children with attachment problems require special attention during and after the adoption process. Presents actions needed to increase the probability that such children can be successfully adopted, detailed attachment patterns, and parenting strategies and…

  8. DETEKSI MUTASI V1016G PADA GEN VOLTAGE-GATED SODIUM CHANNEL PADA POPULASI Aedes aegypti (DIPTERA: CULICIDAE DI KABUPATEN KLATEN, JAWA TENGAH DENGAN METODE ALLELE-SPECIFIC PCR

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    Dyah Widiastuti

    2015-10-01

    Full Text Available AbstrakMeluasnya kejadian resistensi pada vektor virus Dengue di Jawa Tengah memerlukan strategi pengelolaan resistensi insektisida secara efektif. Oleh karena itu, informasi mengenai mutasi gen pada posisi 1016 di domain II segmen ke­6 gen VGSC pada nyamuk Aedes aegypti yang menyebabkan perubahan asam amino valin (V menjadi glisin (G akan dapat memperkuat penelitian operasional mengenai strategi pemilihan insektisida dalam program­pengendalian­vektor­Dengue.­Penelitian­ini­menggunakan­uji­Allele-Specific­Polymerase­Chain­Reaction(AS­PCR yang dapat mendeteksi mutasi V1016G. Sampel penelitian ini adalah 22 ekor nyamuk Aedes aegypti dari Kabupaten Klaten yang berumur 2­5 hari. Hasil penelitian menunjukkan bahwa 22,7% nyamuk belum mengalami mutasi (V/V, 59,1% nyamuk mengalami mutasi heterozigot (V/G dan 18,2% nyamuk mengalami mutasi homozigot (G/G. Hal ini menunjukkan indikasi terjadinya resistensi populasi nyamuk Ae.aegypti terhadap insektisida sintetik piretroid yang disebabkan oleh mekanisme knockdown resistance.Kata Kunci:­Aedes­aegypti,­mutasi­V1016G,­Allele-Specific­PCR,­VGSCAbstractInsecticides resistance has spread rapidly among dengue vectors from Central Java, and require an effective insecticide resistance management strategies.one of the resistance mechanism in Aedes aegypti may arise through knockdown resistance or kdr which consists of single point mutation within the genes that are targeted by insecticide compounds. Mutation at position 1016 in domain II, segment 6 of the Voltage Gated Sodium Channel gene in Ae. aegypti leads to a valine to glycine substitution (V1016G is associated with resistance to the type II pyrethroid. The result of this study will help us to strengthen basic and operational research on the­development­of­strategies­for­Dengue­vector­control­in­Indonesia.­This­study­utilized­an­allele-specificPolymerase Chain Reaction (AS­PCR assay that could be used to detect the V1016G

  9. To be or not to be the odd one out - Allele-specific transcription in pentaploid dogroses (Rosa L. sect. Caninae (DC. Ser

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    Theißen Günter

    2011-02-01

    Full Text Available Abstract Background Multiple hybridization events gave rise to pentaploid dogroses which can reproduce sexually despite their uneven ploidy level by the unique canina meiosis. Two homologous chromosome sets are involved in bivalent formation and are transmitted by the haploid pollen grains and the tetraploid egg cells. In addition the egg cells contain three sets of univalent chromosomes which are excluded from recombination. In this study we investigated whether differential behavior of chromosomes as bivalents or univalents is reflected by sequence divergence or transcription intensity between homeologous alleles of two single copy genes (LEAFY, cGAPDH and one ribosomal DNA locus (nrITS. Results We detected a maximum number of four different alleles of all investigated loci in pentaploid dogroses and identified the respective allele with two copies, which is presumably located on bivalent forming chromosomes. For the alleles of the ribosomal DNA locus and cGAPDH only slight, if any, differential transcription was determined, whereas the LEAFY alleles with one copy were found to be significantly stronger expressed than the LEAFY allele with two copies. Moreover, we found for the three marker genes that all alleles have been under similar regimes of purifying selection. Conclusions Analyses of both molecular sequence evolution and expression patterns did not support the hypothesis that unique alleles probably located on non-recombining chromosomes are less functional than duplicate alleles presumably located on recombining chromosomes.

  10. Questions about Adoption

    Science.gov (United States)

    ... more about their How-to-Adopt and Adoption Parenting Network . Q: What are the different types of adoption? A: Children can be adopted through the national public child welfare system, private ...

  11. Monitoring Sub-Saharan African physician migration and recruitment post-adoption of the WHO code of practice: temporal and geographic patterns in the United States.

    Science.gov (United States)

    Tankwanchi, Akhenaten Benjamin Siankam; Vermund, Sten H; Perkins, Douglas D

    2015-01-01

    Data monitoring is a key recommendation of the WHO Global Code of Practice on the International Recruitment of Health Personnel, a global framework adopted in May 2010 to address health workforce retention in resource-limited countries and the ethics of international migration. Using data on African-born and African-educated physicians in the 2013 American Medical Association Physician Masterfile (AMA Masterfile), we monitored Sub-Saharan African (SSA) physician recruitment into the physician workforce of the United States (US) post-adoption of the WHO Code of Practice. From the observed data, we projected to 2015 with linear regression, and we mapped migrant physicians' locations using GPS Visualizer and ArcGIS. The 2013 AMA Masterfile identified 11,787 active SSA-origin physicians, representing barely 1.3% (11,787/940,456) of the 2013 US physician workforce, but exceeding the total number of physicians reported by WHO in 34 SSA countries (N = 11,519). We estimated that 15.7% (1,849/11,787) entered the US physician workforce after the Code of Practice was adopted. Compared to pre-Code estimates from 2002 (N = 7,830) and 2010 (N = 9,938), the annual admission rate of SSA émigrés into the US physician workforce is increasing. This increase is due in large part to the growing number of SSA-born physicians attending medical schools outside SSA, representing a trend towards younger migrants. Projection estimates suggest that there will be 12,846 SSA migrant physicians in the US physician workforce in 2015, and over 2,900 of them will be post-Code recruits. Most SSA migrant physicians are locating to large urban US areas where physician densities are already the highest. The Code of Practice has not slowed the SSA-to-US physician migration. To stem the physician "brain drain", it is essential to incentivize professional practice in SSA and diminish the appeal of US migration with bolder interventions targeting primarily early-career (age ≤ 35) SSA physicians.

  12. Analysis of the relationship between genomic GC Content and patterns of base usage, codon usage and amino acid usage in prokaryotes: similar GC content adopts similar compositional frequencies regardless of the phylogenetic lineages.

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    Hui-Qi Zhou

    Full Text Available The GC contents of 2670 prokaryotic genomes that belong to diverse phylogenetic lineages were analyzed in this paper. These genomes had GC contents that ranged from 13.5% to 74.9%. We analyzed the distance of base frequencies at the three codon positions, codon frequencies, and amino acid compositions across genomes with respect to the differences in the GC content of these prokaryotic species. We found that although the phylogenetic lineages were remote among some species, a similar genomic GC content forced them to adopt similar base usage patterns at the three codon positions, codon usage patterns, and amino acid usage patterns. Our work demonstrates that in prokaryotic genomes: a base usage, codon usage, and amino acid usage change with GC content with a linear correlation; b the distance of each usage has a linear correlation with the GC content difference; and c GC content is more essential than phylogenetic lineage in determining base usage, codon usage, and amino acid usage. This work is exceptional in that we adopted intuitively graphic methods for all analyses, and we used these analyses to examine as many as 2670 prokaryotes. We hope that this work is helpful for understanding common features in the organization of microbial genomes.

  13. The − 5 A/G single-nucleotide polymorphism in the core promoter region of MT2A and its effect on allele-specific gene expression and Cd, Zn and Cu levels in laryngeal cancer

    Energy Technology Data Exchange (ETDEWEB)

    Starska, Katarzyna, E-mail: katarzyna.starska@umed.lodz.pl [I Department of Otolaryngology and Laryngological Oncology, Medical University of Łódź, Kopcinskiego 22, 90-153 Łódź (Poland); Krześlak, Anna; Forma, Ewa [Department of Cytobiochemistry, University of Łódź, Pomorska 142/143, 90-236 Łódź (Poland); Olszewski, Jurek [II Department of Otolaryngology and Laryngological Oncology, Medical University of Łódź, Żeromskiego 113, 90-549 Łódź (Poland); Morawiec-Sztandera, Alina [Department of Head and Neck Surgery, Medical University of Łódź, Paderewskiego 4, 93-509 Łódź (Poland); Aleksandrowicz, Paweł [Department of Otolaryngology and Laryngological Oncology, Medical University of Lublin, Jaczewskiego 8, 20-954 Lublin (Poland); Lewy-Trenda, Iwona [Department of Pathology, Medical University of Łódź, Pomorska 251, 92-213 Łódź (Poland); and others

    2014-10-15

    Metallothioneins (MTs) are low molecular weight, cysteine-rich heavy metal-binding proteins which participate in the mechanisms of Zn homeostasis, and protect against toxic metals. MTs contain metal-thiolate cluster groups and suppress metal toxicity by binding to them. The aim of this study was to determine the − 5 A/G (rs28366003) single-nucleotide polymorphism (SNP) in the core promoter region of the MT2A gene and to investigate its effect on allele-specific gene expression and Cd, Zn and Cu content in squamous cell laryngeal cancer (SCC) and non-cancerous laryngeal mucosa (NCM) as a control. The MT2A promoter region − 5 A/G SNP was determined by restriction fragment length polymorphism using 323 SCC and 116 NCM. MT2A gene analysis was performed by quantitative real-time PCR. The frequency of A allele carriage was 94.2% and 91.8% in SCC and NCM, respectively, while G allele carriage was detected in 5.8% and 8.2% of SCC and NCM samples, respectively. As a result, a significant association was identified between the − 5 A/G SNP in the MT2A gene with mRNA expression in both groups. Metal levels were analyzed by flame atomic absorption spectrometry. The significant differences were identified between A/A and both the A/G and G/G genotypes, with regard to the concentration of the contaminating metal. The Spearman rank correlation results showed that the MT2A expression and Cd, Zn, Cu levels were negatively correlated. Results obtained in this study suggest that − 5 A/G SNP in MT2A gene may have an effect on allele-specific gene expression and accumulation of metal levels in laryngeal cancer. - Highlights: • MT2A gene expression and metal content in laryngeal cancer tissues • Association between SNP (rs28366003) and expression of MT2A • Significant associations between the SNP and Cd, Zn and Cu levels • Negative correlation between MT2A gene expression and Cd, Zn and Cu levels.

  14. Development of an allele-specific, loop-mediated, isothermal amplification method (AS-LAMP to detect the L1014F kdr-w mutation in Anopheles gambiae s. l.

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    Badolo Athanase

    2012-07-01

    Full Text Available Abstract Background Malaria control relies heavily on treated bed nets and indoor residual spraying with pyrethroid insecticides. Unfortunately, the resistance to pyrethroid insecticides, mainly due to the kdr mutation, is spreading in the main malaria vector Anopheles gambiae s.l., decreasing the insecticides’ efficacy. To manage the insecticide resistance rapidly and flexibly, simple and effective tools for the early detection of resistant mosquitoes are needed. This study aimed to develop an allele-specific, loop-mediated, isothermal amplification (AS-LAMP method to detect the West African-type kdr mutation (kdr-w; L1014F in field-collected mosquitoes. Methods DNA fragments of the wild-type and the mutated kdr gene were used to select the primers and develop the method. The primers were designed with the mutation at the 5’ end of the backward inner primer (BIP. The AS-LAMP method was compared to the AS-PCR method using the genomic DNA of 120 field-collected mosquitoes. Results The AS-LAMP method could discriminate between the wild-type homozygote, the heterozygote, and the kdr-w homozygote within 75 min. The AS-LAMP method has the advantage of being faster and at least as sensitive and specific as the AS-PCR method. Conclusions The AS-LAMP method can be used to detect the kdr mutation for quick decision-making, even in less well-equipped laboratories.

  15. Adopted Children and Discipline

    Science.gov (United States)

    ... Life Listen Español Text Size Email Print Share Adopted Children & Discipline Page Content Article Body Some parents are hesitant to discipline the child they have adopted. They may set fewer limits than they would ...

  16. Detection of BRAF Mutations Using a Fully Automated Platform and Comparison with High Resolution Melting, Real-Time Allele Specific Amplification, Immunohistochemistry and Next Generation Sequencing Assays, for Patients with Metastatic Melanoma.

    Directory of Open Access Journals (Sweden)

    Alexandre Harlé

    Full Text Available Metastatic melanoma is a severe disease with one of the highest mortality rate in skin diseases. Overall survival has significantly improved with immunotherapy and targeted therapies. Kinase inhibitors targeting BRAF V600 showed promising results. BRAF genotyping is mandatory for the prescription of anti-BRAF therapies.Fifty-nine formalin-fixed paraffin-embedded melanoma samples were assessed using High-Resolution-Melting (HRM PCR, Real-time allele-specific amplification (RT-ASA PCR, Next generation sequencing (NGS, immunohistochemistry (IHC and the fully-automated molecular diagnostics platform IdyllaTM. Sensitivity, specificity, positive predictive value and negative predictive value were calculated using NGS as the reference standard to compare the different assays.BRAF mutations were found in 28(47.5%, 29(49.2%, 31(52.5%, 29(49.2% and 27(45.8% samples with HRM, RT-ASA, NGS, IdyllaTM and IHC respectively. Twenty-six (81.2% samples were found bearing a c.1799T>A (p.Val600Glu mutation, three (9.4% with a c.1798_1799delinsAA (p.Val600Lys mutation and one with c.1789_1790delinsTC (p.Leu597Ser mutation. Two samples were found bearing complex mutations.HRM appears the less sensitive assay for the detection of BRAF V600 mutations. The RT-ASA, IdyllaTM and IHC assays are suitable for routine molecular diagnostics aiming at the prescription of anti-BRAF therapies. IdyllaTM assay is fully-automated and requires less than 2 minutes for samples preparation and is the fastest of the tested assays.

  17. Validation of a Multiplex Allele-Specific Polymerase Chain Reaction Assay for Detection of KRAS Gene Mutations in Formalin-Fixed, Paraffin-Embedded Tissues from Colorectal Cancer Patients.

    Directory of Open Access Journals (Sweden)

    Sirirat Seekhuntod

    Full Text Available Patients with KRAS mutations do not respond to epidermal growth factor receptor (EGFR inhibitors and fail to benefit from adjuvant chemotherapy. Mutation analysis of KRAS is needed before starting treatment with monoclonal anti-EGFR antibodies in patients with metastatic colorectal cancer (mCRC. The objective of this study is to develop a multiplex allele-specific PCR (MAS-PCR assay to detect KRAS mutations.We developed a single-tube MAS-PCR assay for the detection of seven KRAS mutations (G12D, G12A, G12R, G12C, G12S, G12V, and G13D. We performed MAS-PCR assay analysis for KRAS on DNA isolated from 270 formalin-fixed paraffin-embedded (FFPE colorectal cancer tissues. Sequences of all 270 samples were determined by pyrosequencing. Seven known point-mutation DNA samples diluted with wild-type DNA were assayed to determine the limitation of detection and reproducibility of the MAS-PCR assay.Overall, the results of MAS-PCR assay were in good concordance with pyrosequencing, and only seven discordant samples were found. The MAS-PCR assay reproducibly detected 1 to 2% mutant alleles. The most common mutations were G13D in codon 13 (49.17%, G12D (25.83% and G12V (12.50% in codon 12.The MAS-PCR assay provides a rapid, cost-effective, and reliable diagnostic tool for accurate detection of KRAS mutations in routine FFPE colorectal cancer tissues.

  18. High frequency of SLC22A12 variants causing renal hypouricemia 1 in the Czech and Slovak Roma population; simple and rapid detection method by allele-specific polymerase chain reaction.

    Science.gov (United States)

    Gabrikova, Dana; Bernasovska, Jarmila; Sokolova, Jitka; Stiburkova, Blanka

    2015-10-01

    Renal hypouricemia is a rare heterogeneous inherited disorder characterized by impaired tubular uric acid transport with severe complications, such as acute kidney injury. Type 1 and 2 are caused by loss-of-function mutations in the SLC22A12 and SLC2A9 gene, respectively. A cohort of 881 randomly chosen ethnic Roma from two regions in Eastern Slovakia and two regions in the Czech Republic participated. Genomic DNA was isolated from buccal swabs and/or from blood samples. The c.1245_1253del and c.1400C>T genotypes were determined using polymerase chain reaction with allele-specific primers in a multiplex arrangement and/or direct sequencing of exon 7 and 9. Allele frequencies and genotypes were tested for Hardy-Weinberg equilibrium using the Chi-square test. 25 subjects were heterozygous and three were homozygous for the c.1245_1253del, while 92 subjects were heterozygous and two were homozygous for the c.1400C>T. Moreover, two participants were compound heterozygotes. Frequencies of the c.1245_1253del and c.1400C>T variants were 1.87 and 5.56 %, respectively. Our finding confirms an uneven geographical and ethnic distribution of SLC22A12 mutant variants. We found that the c.1245_1253del and c.1400C>T variants were present in the Czech and Slovak Roma population at unexpectedly high frequencies. Renal hypouricemia should be kept in mind during differential diagnostic on Roma patients with low serum uric acid concentrations.

  19. [Attachment and Adoption: Diagnostics, Psychopathology, and Therapy].

    Science.gov (United States)

    Brisch, Karl-Heinz

    2015-01-01

    This presentation describes the development of attachment between adopted children and their adoptive parents with a focus on the particular issues seen in international adoptions. The questions of settling in, trauma in the country of origin, and the motivations of the adoptive parents will be discussed. Diagnosis and various psychopathological manifestations will be examined, as will outpatient and inpatient modes of therapy. The treatment of children of various ages will be covered along with the necessity for intensive counseling and psychotherapy for the adoptive parents. This will enable the parents to work through early trauma, which will give them and their adopted child the basis for developing healthy attachment patterns. This in turn will enable the child to mature and integrate into society. Possibilities of prevention are discussed. Many of the approaches discussed here regarding attachment and adoption may be applied to foster children and their foster parents.

  20. The claim from adoption.

    Science.gov (United States)

    Petersen, Thomas Sobirk

    2002-08-01

    In this article several justifications of what I call 'the claim from adoption' are examined. The claim from adoption is that, instead of expending resources on bringing new children into the world using reproductive technology and then caring for these children, we ought to devote these resources to the adoption and care of existing destitute children. Arguments trading on the idea that resources should be directed to adoption instead of assisted reproduction because already existing people can benefit from such a use of resources whereas we cannot benefit individuals by bringing them into existence are rejected. It is then argued that a utilitarian argument proposed by Christian Munthe that supports the claim from adoption in some situations should be rejected because the support it offers does not extend to certain situations in which it seems morally obvious that resources should be expended on adoption rather than assisted reproduction. A version of the Priority View improves upon Munthe's utilitarianism by supporting the claim from adoption in the cases in which Munthe's argument failed. Some allegedly counterintuitive implications of the Priority View are then discussed, and it is concluded that the Priority View is more plausible than utilitarianism. In a concluding section on policy issues it is argued that, even though the claim from adoption can be justified in a variety of situations, it does not follow that, in these situations, governments should direct resources away from assisted reproduction and towards adoption.

  1. The Danish Adoption Register

    DEFF Research Database (Denmark)

    Petersen, Liselotte; Sørensen, Thorkild I A

    2011-01-01

    The Danish Adoption Register was established in 1963-1964 to explore the genetic and environmental contribution to familial aggregation of schizophrenia.......The Danish Adoption Register was established in 1963-1964 to explore the genetic and environmental contribution to familial aggregation of schizophrenia....

  2. The Colorado Adoption Project.

    Science.gov (United States)

    Plomin, R; DeFries, J C

    1983-04-01

    This report provides an overview of the Colorado Adoption Project (CAP), a longitudinal, prospective, multivariate adoption study of behavioral development. Examples of the types of analyses that can be conducted using this design are presented. The examples are based on general cognitive-ability data for adoptive, biological, and control parents; assessments of their home environment; and Bayley Mental Development Index scores for 152 adopted children and 120 matched control children tested at both 1 and 2 years of age. The illustrative analyses include matched control children tested at both 1 and 2 years of age. The illustrative analyses include examination of genetic and environmental sources of variance, identification of environmental influence devoid of genetic bias, assessment of genotype-environment interaction and correlation, and analyses of the etiology of change and continuity in development.

  3. Adopt Your Watershed

    Data.gov (United States)

    U.S. Environmental Protection Agency — Adopt Your Watershed is a Website that encourages stewardship of the nation's water resources and serves as a national inventory of local watershed groups and...

  4. Becoming an Adoptive Parent

    DEFF Research Database (Denmark)

    McIlvenny, Paul; Raudaskoski, Pirkko Liisa

    , we trace how adopters publicly narrate their own experiences and problems with fertility and with adoption, as well as how they construct their personal websites, network with others locally and internationally, orient to other ‘sites’ or sources of information, share advice and create 'public goods...... those practices which may precipitate a 'call for help' to distant actors, such as social welfare provision or counselling services....

  5. Beyond the adoption/ non-adoption dichotomy: the impact of innovation characteristics on potential adopters' transition through adoption process stages

    NARCIS (Netherlands)

    Agarwal, M.K.; Frambach, R.T.

    2002-01-01

    Research on innovation adoption has suffered from a bias towards understanding the factors that affect the dichotomous adoption/non-adoption decision.Much less attention is devoted to the question why potential adopters fail to progress to the adoption stage from earlier stages in the decision makin

  6. Adoption, nation, migration

    DEFF Research Database (Denmark)

    Müller, Anders Riel

    2013-01-01

    Som transnationalt adopteret vokser man ofte op med en fortælling om, at man er født i et fattigt land. Og at ens første forældre var fattige eller oplevede så store problemer, at de ikke så andre muligheder end at afgive en til adoption. Det er en historie, man bliver fortalt igen og igen. Og so...

  7. Adoption of Broadband Services

    DEFF Research Database (Denmark)

    Falch, Morten

    2008-01-01

    successful markets for broadband. This is done through analysis of national policies in three European countries-Denmark, Sweden, and Germany-and the U.S., Japan, and South Korea. We concluded that successful implementation of broadband depends on the kind of policy measures to be taken at the national level....... Many countries have provided active support for stimulating diffusion of broadband and national variants of this type of policies in different countries are important for an explanation of national differences in adoption of broadband....

  8. Åben Adoption

    DEFF Research Database (Denmark)

    Jeldtoft, Nadia

    2007-01-01

    barn adopteres, jo mere stabil og uproblematisk bliver relationen mellem barn og adoptivforældre. Samtidig peger undersøgelserne på, at adoptioner med høj grad af åbenhed og kontakt mellem barn, adoptivforældrene og de biologiske forældre fungerer bedst. Hermed rokkes ved en udbredt forestilling om......Denne rapport er en systematisk forskningsoversigt over udenlandske erfaringer med adoption i forhold til anbringelse uden for hjemmet, fx familiepleje, institutionsanbringelse og hjemgivelse til de biologiske forældre. Konklusionerne i rapporten er overraskende entydige: Adopterede børn klarer sig...

  9. After adoption: dissolution or permanence?

    Science.gov (United States)

    Festinger, Trudy

    2002-01-01

    Results are presented on the whereabouts of 516 adopted children, based on a random sample of children adopted from placement in New York City in 1996. Data from interviews with adoptive parents were augmented by information from adoption subsidy records and state child tracking files, as well as interviews with caregivers of children whose adoptive parents were deceased. There were few dissolutions, but postadoption service needs were many.

  10. Process of adoption communication openness in adoptive families: adopters’ perspective

    Directory of Open Access Journals (Sweden)

    Maria Acciaiuoli Barbosa-Ducharne

    2016-01-01

    Full Text Available Abstract Communication about adoption is a family interaction process which is more than the simple exchange of information. Adoption communication can be characterized in terms of the level of openness of family conversations regarding the child’s past and the degree of the family’s adoption social disclosure. The objective of this study is to explore the process of adoption communication openness in Portuguese adoptive families by identifying the impact of variables related to the adoption process, the adoptive parenting and the adoptee. One hundred twenty five parents of children aged 3 to 15, who were adopted on average 4 years ago, participated in this study. Data was collected during home visits using the Parents Adoption Process Interview. A cluster analysis identified three different groups of families according to the level of adoption communication openness within the family and outside. The findings also showed that the process of the adoption communication openness started when parents decided to adopt, developed in parent-child interaction and was susceptible to change under professional intervention. The relevance of training given to prospective adopters and of professional practice based on scientific evidence is highlighted.

  11. Adopting EIL in China

    Institute of Scientific and Technical Information of China (English)

    王静

    2007-01-01

    In this paper, I state my views of the global spread of English. Through analysis of the reasons of the wide spread of EIL, I emphasize that adopting varieties of models is vitally important in relation to English teaching and learning in China, despite a number of obstacles still existing. British, American, Australian, Canada, or any other English should be taught compatibly. No matter what varieties we use, intelligibility is most important among people from other cultures with different linguistic background. Pedagogy should also be adjusted to follow the features of EIL aiming to facilitate learners' communication with people from a wide range of countries and to access the vast amount of information currently available in English. Therefore, supportive policy should be made to both raise all people's awareness of English used internationally and guarantee the need from education practice. Curriculum, without doubt, should include varieties of English in addition to British and American English.

  12. The Texas Adoption Project: adopted children and their intellectual resemblance to biological and adoptive parents.

    Science.gov (United States)

    Horn, J M

    1983-04-01

    Intelligence test scores were obtained from parents and children in 300 adoptive families and compared with similar measures available for the biological mothers of the same adopted children. Results supported the hypothesis that genetic variability is an important influence in the development of individual differences for intelligence. The most salient finding was that adopted children resemble their biological mothers more than they resemble the adoptive parents who reared them from birth. A small subset of the oldest adopted children did not resemble their biological mothers. The suggestion that the influence of genes declines with age is treated with caution since other adoption studies report a trend in the opposite direction.

  13. Adoption Resources for Black Children

    Science.gov (United States)

    Gallagher, Ursula M.

    1971-01-01

    The growing number of adoptions in this country, including racially mixed adoptions, attest to the general acceptance of adoption as a way of bringing love to children in need of families of their own and the satisfactions of parenthood to childless couples, single men and women, and families who have room for one more. (Author/AJ)

  14. Adoption Resource Directory: Region X.

    Science.gov (United States)

    1983

    State, regional, and national adoption resources are described in this directory for residents of Region X states (Alaska, Idaho, Oregon, and Washington). Emphasizing the adoption of children with special needs, the directory gives organizational contacts for parents in various stages of the adoption process and mentions resources for social…

  15. Allele-specific suppression of the temperature sensitivity of fitA/fitB mutants of Escherichia coli by a new mutation (fitC4): isolation, characterization and its implications in transcription control

    Indian Academy of Sciences (India)

    S Vidya; B Praveen Kamalakar; M Hussain Munavar; L Sathish Kumar; R Jayaraman

    2006-03-01

    The temperature sensitive transcription defective mutant of Escherichia coli originally called fitA76 has been shown to harbour two missense mutations namely pheS5 and fit95. In order to obtain a suppressor of fitA76, possibly mapping in rpoD locus, a Ts+ derivative (JV4) was isolated from a fitA76 mutant. It was found that JV4 neither harbours the lesions present in the original fitA76 nor a suppressor that maps in or near rpoD. We show that JV4 harbours a modified form of fitA76 (designated fitA76*) together with its suppressor. The results presented here indicate that the fit95 lesion is intact in the fitA76* mutant and the modification should be at the position of pheS5. Based on the cotransduction of the suppressor mutation and/or its wild type allele with pps, aroD and zdj-3124::Tn10 kan we have mapped its location to 39⋅01 min on the E. coli chromosome. We tentatively designate the locus defined by this new extragenic suppressor as fitC and the suppressor allele as fitC4. While fitC4 could suppress the Ts phenotype of fitA76* present in JV4, it fails to suppress the Ts phenotype of the original fitA76 mutant (harbouring pheS5 and fit95). Also fitC4 could suppress the Ts phenotype of a strain harbouring only pheS5. Interestingly, the fitC4 Ts phenotype could also be suppressed by fit95. The pattern of decay of pulse labelled RNA in the strains harbouring fitC4 and the fitA76* resembles that of the original fitA76 mutant implying a transcription defect similar to that of fitA76 in both these mutants. The implications of these findings with special reference to transcription control by Fit factors in vivo are discussed.

  16. Intellectual resemblance among adoptive adoptive and biological relatives: the Texas adoption project.

    Science.gov (United States)

    Horn, J M; Loehlin, J C; Willerman, L

    1979-05-01

    Intellectual and personality measures were available from unwed mothers who gave their children up for adoption at birth. The same or similar measures have been obtained from 300 sets of adoptive parents and all of their adopted and natural children in the Texas Adoption Project. The sample characteristics are discussed in detail, and the basic findings for IQ are presented. Initial analyses of the data on IQ suggest moderate heritabilities. Emphasis is placed on the preliminary nature of these findings.

  17. Technology Adoption: an Interaction Perspective

    Science.gov (United States)

    Sitorus, Hotna M.; Govindaraju, Rajesri; Wiratmadja, I. I.; Sudirman, Iman

    2016-02-01

    The success of a new technology depends on how well it is accepted by its intended users. Many technologies face the problem of low adoption rate, despite the benefits. An understanding of what makes people accept or reject a new technology can help speed up the adoption rate. This paper presents a framework for technology adoption based on an interactive perspective, resulting from a literature study on technology adoption. In studying technology adoption, it is necessary to consider the interactions among elements involved in the system, for these interactions may generate new characteristics or new relationships. The interactions among elements in a system adoption have not received sufficient consideration in previous studies of technology adoption. Based on the proposed interaction perspective, technology adoption is elaborated by examining interactions among the individual (i.e. the user or prospective user), the technology, the task and the environment. The framework is formulated by adopting several theories, including Perceived Characteristics of Innovating, Diffusion of Innovation Theory, Technology Acceptance Model, Task-Technology Fit and usability theory. The proposed framework is illustrated in the context of mobile banking adoption. It is aimed to offer a better understanding of determinants of technology adoption in various contexts, including technology in manufacturing systems.

  18. Prescriber adoption of newly approved selective COX-2 inhibitors

    NARCIS (Netherlands)

    Layton, Deborah; Souverein, Patrick C.; Heerdink, Eibert R.; Shakir, Saad A. W.; Egberts, A. G. C.

    2008-01-01

    Introduction There is no consistent definition of prescribers who adopt new drug treatments early. This study examines if COX-2 inhibitors (coxibs) were prescribed by subsets of practitioners and describes GP adoption patterns of coxibs and existing NSAIDs over time. Methods A population-based drug

  19. Technology adoption and the impact on average productivity

    NARCIS (Netherlands)

    Hellegers, P.J.G.J.; Zeng, D.; Zilberman, D.

    2011-01-01

    In this paper, a framework is developed to analyze how the specifications of new technologies and the heterogeneity of micro-units of production affect the input use, the adoption pattern, and the productivity of inputs. It shows that asset-productivity-enhancing (APE) technologies tend to be adopte

  20. Adopted youth and sleep difficulties

    Directory of Open Access Journals (Sweden)

    Radcliff Z

    2016-12-01

    Full Text Available Zach Radcliff, Allison Baylor, Bruce Rybarczyk Department of Psychology, Virginia Commonwealth University, Richmond, VA, USA Abstract: Sleep is a critical component of healthy development for youth, with cascading effects on youth’s biological growth, psychological well-being, and overall functioning. Increased sleep difficulties are one of many disruptions that adopted youth may face throughout the adoption process. Sleep difficulties have been frequently cited as a major concern by adoptive parents and hypothesized in the literature as a problem that may affect multiple areas of development and functioning in adopted youth. However, there is limited research exploring this relationship. Using a biopsychosocial framework, this paper reviews the extant literature to explore the development, maintenance, and impact of sleep difficulties in adopted youth. Finally, implications for future research and clinical interventions are outlined. Keywords: adoption, sleep, youth

  1. Adoption and Assisted Reproduction. Adoption and Ethics, Volume 4.

    Science.gov (United States)

    Freundlich, Madelyn

    The controversies in adoption have extended across a spectrum of policy and practice issues, and although the issues have become clear, resolution has not been achieved nor has consensus developed regarding a framework on which to improve the quality of adoption policy and practice. This book is the fourth in a series to use an ethics-based…

  2. Homosexuality and adoption in Brazil.

    Science.gov (United States)

    Uziel, A P

    2001-11-01

    Western societies are undergoing legal and policy changes in relation to laws governing the family, marital status, sexual orientation and the welfare of children, including in Brazil where, in the 1990s, the rights of homosexuals were incorporated into ongoing debates about what constitutes a family. This paper discusses the issue of adoption of children by homosexual men in Brazil, using information from court records from 1995-2000 in Rio de Janeiro, and from interviews with two judges, five psychologists and four social workers who evaluate those wishing to adopt. It uses the case records of one man's application to adopt, in which homosexuality became a central issue. Both the construction of masculinity in relation to parenting and concepts of the family were the parameters upon which the decision to allow him to adopt or not depended. Because the legislation does not specify what the sexual orientation of would-be adoptive parents should be, it is possible for single persons to adopt if they show they can be good parents. As more single people, alone or in couples, seek to adopt, it is important to clarify the criteria for judicial decisions on adoption applications. A dialogue is therefore needed on the meaning of family and whether and how it relates to sexual orientation. It is only on this basis that the courts can take a clear decision as to whether being homosexual is a relevant issue in regard to applications to adopt or not.

  3. Faculty Adoption of Educational Technology

    Science.gov (United States)

    Moser, Franziska Zellweger

    2007-01-01

    Although faculty support has been identified as a critical factor in the success of educational-technology programs, many people involved in such efforts underestimate the complexities of integrating technology into teaching. In this article, the author proposes an adoption cycle to help tackle the complex issue of technology adoption for…

  4. Healthy Travel for International Adoptions

    Centers for Disease Control (CDC) Podcasts

    2007-10-22

    The number of international adoptions, many from developing countries, has doubled in the last 10 years. This podcast discusses ways adoptive families can protect their own health and the health of their new children.  Created: 10/22/2007 by National Center for the Prevention, Detection and Control of Infectious Diseases (NCPDCID).   Date Released: 10/24/2007.

  5. Intra-Firm Adoption Decisions

    NARCIS (Netherlands)

    Y.M. van Everdingen (Yvonne); B. Wierenga (Berend)

    2001-01-01

    textabstractThe subject of this paper is intra-firm adoption decisions, a relatively unexplored research area in the marketing literature. In particular, we investigate which factors influence the intra-firm adoption decisions regarding the common European currency of the treasury, purchasing and sa

  6. Parents’ Feelings Towards Their Adoptive and Non-Adoptive Children

    Science.gov (United States)

    Glover, Marshaun B.; Mullineaux, Paula Y.; Deater-Deckard, Kirby; Petrill, Stephen A.

    2010-01-01

    In the current study, we examined parent gender differences in feelings (negativity and positivity) and perceptions of child behavioural and emotional problems in adoptive and biological parent–child dyads. In a sample of 85 families, we used a novel within-family adoption design in which one child was adopted and one child was a biological child of the couple, and tested whether the links between parent feelings and child maladjustment included effects of passive gene–environment correlation. Parents reported more negativity and less positivity as well as higher levels of externalizing behaviour for the adopted child compared to the non-adopted child, although effect sizes were small and no longer statistically significant after correcting for multiple comparisons. Fathers and mothers did not differ significantly in their reports of positive and negative feelings towards their children or in regard to child externalizing and internalizing behaviours. The correlations between parental negativity and positivity and child externalizing and internalizing were similar for fathers and mothers, and for adopted and non-adopted children. The findings suggest similar parent–child relationship processes for fathers and mothers, and that genetic transmission of behaviour from parent to child does not account for the association between parental warmth and hostility and child-adjustment problems. PMID:21088705

  7. Development of allele-specific PCR for detection of herbicide-resistance in Monochoria korsakowii Regel et Maack%采用ASPCR技术检测抗药性雨久花ALS突变基因碱基种类的探讨

    Institute of Scientific and Technical Information of China (English)

    吴明根; 金万赫; 许勇男

    2011-01-01

    等位基因特异性扩增(Allele-specific PCR简称:ASPCR)技术是一项快速、简便、低成本的检测等位基因单核苷酸多态性的新方法.作者针对雨久花感抗磺酰脲类生态型ALS基因编码区第592位碱基密码(ALS氨基酸第197位)特征,采用在引物3'末端设有胸腺嘧啶核苷酸(感性)或鸟嘌呤(抗性)核苷酸的2种引物,对延边稻田发生的雨久花感抗磺酰脲类生态型ALS基因编码区第592位进行了ASPCR.检测结果显示,当以引物3'末端设有胸腺嘧啶引物时,感性类型出现ASPCR扩增带,而抗性类型无带出现,反之抗性类型有带而感性类型无带,检测到延边稻田发生的抗磺酰脲类雨久花生态型的ALS 197位点的脯氨酸被组氨酸替代,证明了该方法的有效性和可靠性.%Allele-specific PCR is a rapid, simple, and low-cost method for single-nucleotide polymorphism identification. Using this technique, a single-nucleotide polymorphism primer marker of thymine(susceptible) or guanine(resistant) at the 3'end on 592 point of ALS(acetolactate synthetase) gene encoding site (197 point of ALS amino acid) was designed to detect the herbicide-resistant type of Monochoria korsakowii Regel et Maack. in Yanbian region. The result shows that susceptibility primer marker can be used to detect the susceptibility biotype;otherwise can be detected resistant biotype; the cause of resistance to sulfonylurea herbicide type can be induced by substitution of Pro197 to histidine. It proved that the technique was efficient and feasible to detect ALS gene of herbicide-resistant type.

  8. Multimodal Resources in Transnational Adoption

    DEFF Research Database (Denmark)

    Raudaskoski, Pirkko Liisa

    The paper discusses an empirical analysis which highlights the multimodal nature of identity construction. A documentary on transnational adoption provides real life incidents as research material. The incidents involve (or from them emerge) various kinds of multimodal resources and participants...

  9. How adoption speed affects the abandonment of cultural tastes.

    Science.gov (United States)

    Berger, Jonah; Le Mens, Gaël

    2009-05-19

    Products, styles, and social movements often catch on and become popular, but little is known about why such identity-relevant cultural tastes and practices die out. We demonstrate that the velocity of adoption may affect abandonment: Analysis of over 100 years of data on first-name adoption in both France and the United States illustrates that cultural tastes that have been adopted quickly die faster (i.e., are less likely to persist). Mirroring this aggregate pattern, at the individual level, expecting parents are more hesitant to adopt names that recently experienced sharper increases in adoption. Further analysis indicate that these effects are driven by concerns about symbolic value: Fads are perceived negatively, so people avoid identity-relevant items with sharply increasing popularity because they believe that they will be short lived. Ancillary analyses also indicate that, in contrast to conventional wisdom, identity-relevant cultural products that are adopted quickly tend to be less successful overall (i.e., reduced cumulative adoption). These results suggest a potential alternate way to explain diffusion patterns that are traditionally seen as driven by saturation of a pool of potential adopters. They also shed light on one factor that may lead cultural tastes to die out.

  10. Open adoption and adoptive mothers: attitudes toward birthmothers, adopted children, and parenting.

    Science.gov (United States)

    Lee, J S; Twaite, J A

    1997-10-01

    The nature and extent of contact between 238 adoptive mothers and their child's biological mother was assessed for the period prior to the birth of the child and during the first two years of the child's life. Adoptive mothers who reported such contact prior to the child's birth had significantly more favorable attitudes toward both the biological mother and the adopted child. Those with contact either before or after the birth also demonstrated significantly more favorable parenting attitudes. Policy implications and the need for further research are noted.

  11. 等位基因特异性引物延伸法在婴儿型和幼儿型神经元蜡样质脂褐质沉积病产前诊断中的应用%Prenatal diagnostic testing for infantile and late-infantile neuronal ceroid lipofusinoses (NCL) using allele specific primer extension (ASPE)

    Institute of Scientific and Technical Information of China (English)

    Nanbert ZHONG; Weina JU; Dorota MOROZIEWICZ; Anetta WRONSKA; Marilyn LI; Krystyna WISNIEWSKI; Susan Sklower BROOKS; Edmund JENKINS; W. Ted BROWN

    2005-01-01

    SUMMARY Infantile (INCL, NCL1) and late-infantile (LINCL, NCL2) neuronal ceroid lipofuscinoses have been found to result from genetic deficiency of genes CLN1 and CLN2, respectively. The application of molecular analyses can facilitate prenatal diagnosis for families affected by NCL1 or NCL2, in which the familial mutation(s) have been identified. Molecular testing with allele-specific primer extension and DNA sequencing was performed in nine pregnancies, four from two NCL1 families and five from five NCL2 families. Lysosomal enzyme activity assays were carried out as well.Four fetuses from three pregnancies in NCL1 families were found to be carriers for a mutation 451C-T in the CLN1 gene and one was normal. Prenatal testing of three NCL2 families who carried mutation R208X in the CLN2 gene showed that all fetuses were carriers. In NCL2 families who carried either mutation IVS5-1C or/and IVS5-1A two normal pregnancies were detected. Our studies indicate that DNA testing, which may provide definitive prenatal diagnosis for NCL, may be used in combination with lysosomal enzyme activity analyses.

  12. Personality disorders in adopted versus non-adopted adults.

    Science.gov (United States)

    Westermeyer, Joseph; Yoon, Gihyun; Amundson, Carla; Warwick, Marion; Kuskowski, Michael A

    2015-04-30

    The goal of this epidemiological study was to investigate lifetime history and odds ratios of personality disorders in adopted and non-adopted adults using a nationally representative sample. Data, drawn from the National Epidemiological Survey on Alcohol and Related Conditions (NESARC), were compared in adopted (n=378) versus non-adopted (n=42,503) adults to estimate the odds of seven personality disorders using logistic regression analyses. The seven personality disorders were histrionic, antisocial, avoidant, paranoid, schizoid, obsessive-compulsive, and dependent personality disorder. Adoptees had a 1.81-fold increase in the odds of any personality disorder compared with non-adoptees. Adoptees had increased odds of histrionic, antisocial, avoidant, paranoid, schizoid, and obsessive-compulsive personality disorder compared with non-adoptees. Two risk factors associated with lifetime history of a personality disorder in adoptees compared to non-adoptees were (1) being in the age cohort 18-29 years (but no difference in the age 30-44 cohort), using the age 45 or older cohort as the reference and (2) having 12 years of education (but no difference in higher education groups), using the 0-11 years of education as the reference. These findings support the higher rates of personality disorders among adoptees compared to non-adoptees.

  13. SMEs' E-commerce Adoption

    DEFF Research Database (Denmark)

    Scupola, Ada

    2009-01-01

    Purpose - Proposes providing an insight about factors affecting business-to-business e-commerce adoption and implementation in small to medium-sized enterprises (SMEs), highlighting similarities and differences between Danish and Australian SMEs.    Design/methodology/approach - The research...... is based on a wide literature review, focused on proposing a theoretical model of technological, environmental and organizational factors influencing e-commerce adoption and implementation. Subsequently, a questionnaire based on the research model has been developed and face-to-face interviews were...... conducted in Danish and Australian companies. Findings - The findings both corroborate previous results about significant factors affecting SMEs' b-to-b e-commerce adoption and implementation and provide new, interesting insights. The study also finds many similarities and differences between Denmark...

  14. Adopting to Agile Software Development

    Directory of Open Access Journals (Sweden)

    Linkevics Gusts

    2014-12-01

    Full Text Available Agile software development can be made successful, but there is no well-defined way how to achieve this. The problem is that the successful adoption of agile methods and practices is a complex process and this process should be customizable for a particular organization or a team. This research focuses on identification of agile methods and practices. Result of the research is the ranking of agile methods, practices and their usage trends. As some terms of agile software development are interpreted differently in different organizations and teams, terminology used is analyzed. Results of the research can be used as a reference material for those who are planning to adopt agile methods or are looking for the next agile practice to implement. Results will be used for the development of an expert system to support agile adoption.

  15. Has the Academy Adopted TQM?

    Science.gov (United States)

    Birnbaum, Robert; Deshotels, Judy

    1999-01-01

    A survey of 469 colleges and universities assessed the degree to which colleges and universities have adopted total quality management (TQM) or continuous quality improvement (CQI) techniques. Results suggest use of TQM/CQI is lower than predicted, at about 13% of institutions. Variations in extent of use of the approach are discussed. (MSE)

  16. Why Adoption of Standards Matters

    Science.gov (United States)

    Journal of Staff Development, 2016

    2016-01-01

    A total of 39 states have adopted, adapted, or endorsed the Standards for Professional Learning, including the standards issued in 2011 (labeled in red) and those published earlier (labeled in blue). Making a commitment to the standards is a commitment to continuous learning for all educators in a school.

  17. ADOPT COMMUNICATIVE APPROACHES IN CET

    Institute of Scientific and Technical Information of China (English)

    LinSitan

    2004-01-01

    How to make CET tailored to the requirements of thesociety is a topic of great significance. This paper is meant toexplore the advantages of adopting communicative approaches inCET( College English Teaching ) by comparing communicativeapproaches with traditional ones and presenting our practice ofemploying communicative approaches as well as the result.

  18. Chinese adoption: practices and challenges.

    Science.gov (United States)

    Lihua, H

    2001-01-01

    The majority of children in China who are the subject of protective services are either abandoned or disabled. Recent reform efforts in China's child welfare practices have focused on the importance of providing safe, permanent families for children in lieu of long-term institutional care. Although challenges still exist, adoption and foster care are increasingly being seen as viable alternatives for these children.

  19. Predicting Virtual Learning Environment Adoption

    DEFF Research Database (Denmark)

    Penjor, Sonam; Zander, Pär-Ola Mikael

    2016-01-01

    This study investigates the significance of Rogers’ Diffusion of Innovations (DOI) theory with regard to the use of a Virtual Learning Environment (VLE) at the Royal University of Bhutan (RUB). The focus is on different adoption types and characteristics of users. Rogers’ DOI theory is applied...

  20. La gestion des adoptions internationales

    OpenAIRE

    Boéchat, Hervé

    2011-01-01

    Après les rumeurs nées lors du tsunami et le désastre avéré de L’Arche de Zoé, l’adoption – et plus généralement le sort des enfants – est devenue une préoccupation majeure en cas de catastrophe naturelle ou de conflit. Haïti, pays depuis longtemps ouvert à l’adoption internationale, n’a pas échappé à la polémique. Hervé Boéchat nous livre l’appréciation de son institution sur cet épisode qui n’a sans doute pas contribué à apaiser le ressentiment des Haïtiens contre la « communauté internati...

  1. Sharing Experiences of Intercountry Adoption

    DEFF Research Database (Denmark)

    Raudaskoski, Pirkko; McIlvenny, Paul

    are interactionally occasioned. Our special concern is with how the past is built into the present through memory work that is actualised and performed not just through language but also through embodied attention such as body posture, gaze and gestures. In other words, we study how the past is folded into the now...... the perspective of the kinds of work that talking about the past does in a specific interaction. Middleton & Brown (2005) extend this interactional perspective to include, on the one hand, the experience of being in the world (ie. duration), and, on the other hand, the use of mediational means (eg. language...... of this research is to better understand the relationships between multimodality, embodiment, interaction and affect. We present the results of our analysis of how the experiences of adoptive parents are (re)mediated in a Danish television documentary series following five prospective adoptive couples, not all...

  2. Revision of the Adoption Law

    Institute of Scientific and Technical Information of China (English)

    1999-01-01

    DECEMBER 29, 1991, saw thePresident of the People’sRepublic of China promulgatea new adoption law by Order 54, a lawwhich was introduced to the seventhNational People’s Congress at thetwenty-third meeting of its StandingCommittee and which took effect fromApril 1, 1992. This was the first suchadoption law in China and, althoughadoption did occur prior to itsimplementation, it was not then boundby legal measures or guarantee.

  3. IDT-TAM Integrated Model for IT Adoption

    Institute of Scientific and Technical Information of China (English)

    ZHANG Nan; GUO Xunhua; CHEN Guoqing

    2008-01-01

    Two classical theories, the technology acceptance model (TAM) and the innovation diffusion theory (IDT), were integrated into a model for analyzing individual information technology (IT) adoption behavior. In this IDT-TAM framework, the perceived characteristics of innovation are categorized as subjective evaluation, objective conditions, and interaction factors. The cognitive mechanisms that drive user IT acceptance are analyzed based on the patterns through which these characteristics influence individual user IT adoption behavior. The model was tested using an empirical survey regarding the adoption and use of e-mail in China. Results from a structural equation model analysis illustrate that the model provides meaningful insights for understanding, explaining, and predicting the IT adoption behavior of Chinese users.

  4. Social Capital Framework in the Adoption of E-Learning

    Science.gov (United States)

    Barton, Siew Mee

    2013-01-01

    This is a study of the influence of social and cultural factors on the adoption of e-learning in higher education in Malaysia, Indonesia, Turkey, Singapore and Australia. Particular attention in each case was given to factors relating to social capital, attitudes and patterns of behavior in leadership, entrepreneurialism, and teaching and to…

  5. Adopted: A practical salinity scale

    Science.gov (United States)

    The Unesco/ICES/SCOR/IAPSO Joint Panel on Oceanographic Tables and Standards has recommended the adoption of a Practical Salinity Scale, 1978, and a corresponding new International Equation of State of Seawater, 1980. A full account of the research leading to their recommendation is available in the series Unesco Technical Papers in Marine Science.The parent organizations have accepted the panel's recommendations and have set January 1, 1982, as the date when the new procedures, formulae, and tables should replace those now in use.

  6. Policy Issues in Gay and Lesbian Adoption.

    Science.gov (United States)

    Sullivan, Ann

    1995-01-01

    Notes that adoption agencies have developed few specific policies on the issue of lesbian and gay adoption. Provides an overview of key considerations about homosexual adopters, including beliefs and values of agency professionals, the legal and social ramifications of adoption into a relationship not based on marriage, and possible consequences…

  7. Comparison of CYP2D6 genotyping by allele-specific PCR with DXT phe-notype and gene chip testing%CYP2D6 PCR基因型与DXT表型和基因芯片检测的比较

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    目的:为了评价CYP2D6的基因型和表型的联系以及基因芯片在CYP2D6多基因分析中的应用.方法:242健康志愿者,口服dextromethorphan后收集尿液测定其代谢率,收集20ml血提取DNA,并通过基因特异性PCR和/(或)基因芯片分析CYP2D6*2--*11,*17和多拷贝CYP2D6基因,其中5个基因(*3、*4、*6、*7和*9)用PCR和CYD450基因芯片同时分析.结果:CYP2D6基因型比表型更富有信息和更能反映CYP2D6酶的表达.CYP2D6*3、*4、*6、*7和*9的基因检测在CYP450基因芯片和基因特异性PCR中显示高度的一致性.结论:基因芯片在检测基因多位点的多基因中是一个有发展前途和可靠的方法.%To evaluate association of genotype and phenotype of CYP2D6 and the application of oligonucleotide microarray hybridization genetic testing in CYP2D6 multiple alleles analyses. METHODS: Two hundred forty-two healthy volunteers were recruited, and a 60 mg oral dose of dextromethorphan (DXT) was administered to each for assessment of the DXT metabolic ratio [ MR]. A 20 ml blood sample was also collected for DNA isolation and testing. CYP2D6 alleles * 2-*11; * 17 and multiple CYP2D6 gene copies were tested by allele-specific PCR and/or the affymetrix CYP450 gene chip assay. Five of the CYP2D6 alleles ( * 3, * 4, *6, * 7, and * 9) were evaluated by both PCR and the CYP450 gene chip assay. RESULTS: The CYP2D6genotype was more informative and reflective in CYP2D6 enzyme expression than a phenotype. Genetic tests for the CYP2D6 * 3, * 4, * 6, * 7 and * 9 alleles showed a high degree of concordance between the CYP450 gene chip and AS-PCR methods. CONCLUSION: Oligonucleotide microarray hybridization is a promising and reliable approach for detecting multiple alleles at gene loci.

  8. [Adoptive parents' satisfaction with the adoption experience and with its impact on family life].

    Science.gov (United States)

    Sánchez-Sandoval, Yolanda

    2011-11-01

    In this study, we discuss the relevance of adoptive families' satisfaction in the assessment of adoption processes. The effects of adoption on a sample group of 272 adoptive families are analyzed. Most families show high levels of satisfaction as to: their decision to adopt, the features of their adopted children and how adoption has affected them as individuals and as a family. Statistical analyses show that these families can have different satisfaction levels depending on certain features of the adoptees, of the adoptive families or of their educational style. Life satisfaction of the adoptees is also related to how their adoptive parents evaluate the adoption.

  9. Electronic Dental Records System Adoption.

    Science.gov (United States)

    Abramovicz-Finkelsztain, Renata; Barsottini, Claudia G N; Marin, Heimar Fatima

    2015-01-01

    The use of Electronic Dental Records (EDRs) and management software has become more frequent, following the increase in prevelance of new technologies and computers in dental offices. The purpose of this study is to identify and evaluate the use of EDRs by the dental community in the São Paulo city area. A quantitative case study was performed using a survey on the phone. A total of 54 offices were contacted and only one declinedparticipation in this study. Only one office did not have a computer. EDRs were used in 28 offices and only four were paperless. The lack of studies in this area suggests the need for more usability and implementation studies on EDRs so that we can improve EDR adoption by the dental community.

  10. Amplificação gênica alelo-específica na caracterização das hemoglobinas S, C e D e as interações entre elas e talassemias beta Allele-specific genic amplification in the characterization of hemoglobins S, C, D and interactions among them and with beta thalassemia

    Directory of Open Access Journals (Sweden)

    Luciane Cristina Bertholo

    2006-08-01

    possible interactions, based on the allele-specific genic amplification (PCR-AE with the use in parallel two primers that differ at their 3’ extremities and are complementary to the normal or mutated sequences. RESULTS AND DISCUSSION: The results make evident the validation of this methodology in the characterization of these mutations, once this procedure is easy to execute,to reproduce, as well as it is possible to be applied to a significative number of samples.

  11. Macro influencers of electronic health records adoption.

    Science.gov (United States)

    Raghavan, Vijay V; Chinta, Ravi; Zhirkin, Nikita

    2015-01-01

    While adoption rates for electronic health records (EHRs) have improved, the reasons for significant geographical differences in EHR adoption within the USA have remained unclear. To understand the reasons for these variations across states, we have compiled from secondary sources a profile of different states within the USA, based on macroeconomic and macro health-environment factors. Regression analyses were performed using these indicator factors on EHR adoption. The results showed that internet usage and literacy are significantly associated with certain measures of EHR adoption. Income level was not significantly associated with EHR adoption. Per capita patient days (a proxy for healthcare need intensity within a state) is negatively correlated with EHR adoption rate. Health insurance coverage is positively correlated with EHR adoption rate. Older physicians (>60 years) tend to adopt EHR systems less than their younger counterparts. These findings have policy implications on formulating regionally focused incentive programs.

  12. Are You Pregnant and Thinking about Adoption?

    Science.gov (United States)

    ... reactions for birth parents after they place their child for adoption. Some birth parents also go through phases of feeling guilty and ... org/ Open Adoption Bloggers http: / / openadoptionbloggers. com Publications Child ... from: https: / / www. childwelfare. gov/ pubs/ f_ ...

  13. Impact of Adoption on Birth Parents

    Science.gov (United States)

    ... best interests ahead of their own when they make the decision to place the child for adoption (Child Welfare Information Gateway, 2007). Other reasons birth parents place their children for adoption include societal and ...

  14. THE URBAN NEGRO AND ADOPTION OF CHILDREN.

    Science.gov (United States)

    DEASY, LEILA CALHOUN; QUINN, OLIVE WESTBROOKE

    THIS STUDY OF THE ATTITUDES OF NEGROES TOWARD THE ADOPTION OF NEGRO CHILDREN IS BASED ON INTERVIEWS WITH POTENTIAL ADOPTIVE COUPLES IN BALTIMORE AND WASHINGTON, D.C. THE RESEARCH SOUGHT TO ASCERTAIN THE REASONS FOR THE VERY LOW ADOPTION RATE OF NEGRO CHILDREN IN THE TWO CITIES. THE SAMPLE CONSISTED OF 484 ECONOMICALLY STABLE PEOPLE BETWEEN THE…

  15. Adoptive immunotherapy of advanced melanoma.

    Science.gov (United States)

    Shapira-Frommer, Ronnie; Schachter, Jacob

    2012-09-01

    Adoptive cell therapy (ACT) has emerged as an effective therapy for patients with metastatic melanoma. Since the first introduction of the protocol in 1988 [1], major improvements have been achieved with response rates of 40%-72% among patients who were resistant to previous treatment lines. Both cell product and conditioning regimen are major determinants of treatment efficacy; therefore, developing ACT protocols explore diverse ways to establish autologous intra-tumoral lymphocyte cultures or peripheral effector cells as well as different lymphodepleting regimens. While a proof of feasibility and a proof of concept had been established with previous published results, ACT will need to move beyond single-center experiences, to confirmatory, multi-center studies. If ACT is to move into widespread practice, it will be necessary to develop reproducible high quality cell production methods and accepted lymphodepleting regimen. Two new drugs, ipilimumab (Yervoy, Bristol-Myers Squibb) and vemurafenib (Zelboraf, Roche), were approved in 2011 for the treatment of metastatic melanoma based on positive phase III trials. Both drugs show a clear overall survival benefit, so the timing of when to use ACT will need to be carefully thought out. In contrast to these 2 new, commercially available outpatient treatments, ACT is a personally-specified product and labor-intensive therapy that demands both acquisition of high standard laboratory procedures and close clinical inpatient monitoring during treatment. It is unique among other anti-melanoma treatments, providing the potential for a durable response following a single, self-limited treatment. This perspective drives the efforts to make this protocol accessible for more patients and to explore modifications that may optimize treatment results.

  16. Heteronormative consensus in the Norwegian same-sex adoption debate?

    Science.gov (United States)

    Anderssen, Norman; Hellesund, Tone

    2009-01-01

    This article investigates the Norwegian newspaper debate (1998-2002) on the right of homosexual couples to adopt children. It identifies two patterns of meaning within which both anti-adoption and pro-adoption sides of the debate were located: 1) the nuclear family as reference point; and 2) a focus on innate qualities. Parallell to a continuous liberalization of sexualities in Norway we seem to witness a consensus on heteronormativity in Norway on both sides of the debate as the basic axiom in public discussions on homosexuality and adoption. In this article, we explore the nature of the heteronormative arguments and the reason for their appearance in this particular debate. The two patterns of meaning reproduce a perception of lesbians and gays as either a worthy or unworthy minority. These findings may be seen as reflecting fundamental positions regarding the Norwegian modernization project, where both sides of the debate see homosexuality as a central symbol. State feminism may also have played the role of reinforcing gender categories and thereby served as an important condition of possibility for contemporary heteronormativity.

  17. Adoption of mobile services : moderating effects of service’s information intensity

    OpenAIRE

    Sendecka, Lenka

    2006-01-01

    The increased use of mobile technology has been dramatic in the recent years. Understanding some of the reasons for this immense expansion lies in comprehending the factors behind consumer usage patterns. Hence it is becoming more and more crucial for today’s marketers to study how and why consumers adopt (new) technology and mobile services. The knowledge of adoption process and the factors driving it may be relevant for both providers and customers. Several models for adoption of informatio...

  18. Simultaneous Versus Sequential Complementarity in the Adoption of Technological and Organizational Innovations

    DEFF Research Database (Denmark)

    Battisti, Giuliana; Rabbiosi, Larissa; Colombo, Massimo G.

    2015-01-01

    It is generally suggested that technological and organizational innovations, being complementary, need to be adopted simultaneously. Nevertheless, sequential rather than simultaneous adoption of these two types of innovation may be optimal. In this paper, we analyze the pattern of mutual causation...... of technological and organizational innovations and contribute to the understanding of their interdependencies......

  19. Evaluation of Relevances on Treating Irritable Bowel Syndrome with Diarrhea Patients by Transcutaneous Electrical Acupoint Stimulation Adopting Traditional Chinese Medicine Pattern Effect Scale%中医证候量表评价TEAS治疗腹泻型肠易激综合征的相关性研究

    Institute of Scientific and Technical Information of China (English)

    陈明显; 叶开升; 金曼

    2013-01-01

    To evaluate the relevances in the treatment of irritable bowel syndrome with diarrhea ( IBS - D) patients by transcutaneous electrical acupoint stimulation (TEAS) adopting Traditional Chinese Medicine pattern effect scale (TCM - PES). Methods; 110 IBS - D patients were randomly divided into 2 groups. The treatment group was stimulated 3 acupoints(Taichong,Yinlingquan and Tianshu)by TEAS twice a day. The control group was simulated for TEAS by stimulating the same acupoints. The treatment lasted for 4 weeks for each group. IBS bowel symptom severity scale (IBS - BSS) ,IBS defecation state questionnaire( IBS - DSQ) ,IBS quality of life questionnaire(IBS - QOL) were measured to evaluate the relevances with TCM - PES. The TCM pattern effect was evaluated by TCM - PES before and 4 weeks after the treatment respectively. Results ;The result showed IBS - BSS, abdominal pain severity, abdominal pain days, abdominal distensions, bowel habit satisficing and life interference had the positive correlation with TCM - PES(P < 0.05,P<0.01). And IBS-QOL, sleep .energy and social role had the negative correlation with TCM - PES(P <0. 05, P<0.01). The TCM -PES result showed TEAS group had more effective than control group in the aspects of primary symptoms (diarrhea, abdominal pain, sternocostal flatulence) and secondary symptoms ( abdominal fullness and distention, depression and irritability, borborygmus and fart gas, belching, sighs ) ( P < 0.05, P < 0. 01). Conclusion; For IBS - D patients, bowel symptom severity and quality of life had the correlations with TCM pattern. TCM - PES could evaluate the pattern effect of IBS - D patients with pattern of the liver - qi invading the spleen. TEAS could improve the pattern effect of IBS - D patients.%目的:应用中医证候量表评价经皮穴位电刺激法(TEAS)治疗腹泻型肠易激综合征(IBS-D)的相关性.方法:110例IBS-D患者随机分为两组,治疗组予TEAS刺激太冲、阴陵泉和天枢三穴,每天2

  20. "Adoption Wisdom: A Guide to the Issues and Feelings of Adoption" by Marlou Russell. Book Review.

    Science.gov (United States)

    Simonson, Tracy

    1998-01-01

    Reviews "Adoption Wisdom: A Guide to the Issues and Feelings of Adoption" by Marlou Russell. Maintains that its narrative/comment format and accounts of those involved in adoption validate the feelings of the adoption-triad members. Suggests that the book lacks information on individuals who contributed comments and contains few narratives from…

  1. THE INTRAPERSONAL CONFLICT PARTICULARITIES OF POTENTIAL ADOPTERS

    Directory of Open Access Journals (Sweden)

    Inga RUSSU

    2016-11-01

    Full Text Available This article addresses a contemporary issue for the Republic of Moldova concerning psychological implications of the adoption process for prospective adopters. This demand is due to the fact that the majority of couples or people passing through the decision making adoption process are facing a number of conflicting emotions and spiritual states. Thereby, is significant to understand the adoption multidimensionality of social and intrapersonal components. Adoptive parents can feel themselves as victims of „the process”. For them, the assessment of living conditions, the assessment of moral and social guarantees, inclusion or exclusion as eligible adoptive parents, represent a procedure part, which generates frustrations and anxieties. Some of them, often suffer from lack of supporting and understanding, as family members point of view, friends and known people. Also, in this context, parents may face some frustrations related to the matching procedure harmonisation with long cogitation process of the child.

  2. The intrapersonal conflict particularities of potential adopters.

    Directory of Open Access Journals (Sweden)

    Inga Russu

    2016-11-01

    Full Text Available This article addresses a contemporary issue for the Republic of Moldova concerning psychological implications of the adoption process for prospective adopters. This demand is due to the fact that the majority of couples or people passing through the decision making adoption process are facing a number of conlicting emotions and spiritual states. Thereby, is signiicant to understand the adoption multidimensionality of social and intrapersonal components. Adoptive parents can feel themselves as victims of „the process”. For them, the assessment of living conditions, the assessment of moral and social guarantees, inclusion or exclusion as eligible adoptive parents, represent a procedure part, which generates frustrations and anxieties. Some of them, often suffer from lack of supporting and understanding, as family members point of view, friends and known people. Also, in this context, parents may face some frustrations related to the matching procedure harmonisation with long cogitation process of the child.

  3. ICT Adoption in Facilities Management Supply Chain

    DEFF Research Database (Denmark)

    Scupola, Ada

    2012-01-01

    This article involves a qualitative study of factors impacting the adoption of ICT solutions in the Danish facility management supply chain. The results show that there are a number of drivers and barriers that influence the adoption of ICT solutions in this service sector. These have been grouped...... concerned with ICT adoption, operations and service management (especially facilities management) as well as operation managers and ICT managers....

  4. Measures for Predictors of Innovation Adoption.

    Science.gov (United States)

    Chor, Ka Ho Brian; Wisdom, Jennifer P; Olin, Su-Chin Serene; Hoagwood, Kimberly E; Horwitz, Sarah M

    2015-09-01

    Building on a narrative synthesis of adoption theories by Wisdom et al. (2013), this review identifies 118 measures associated with the 27 adoption predictors in the synthesis. The distribution of measures is uneven across the predictors and predictors vary in modifiability. Multiple dimensions and definitions of predictors further complicate measurement efforts. For state policymakers and researchers, more effective and integrated measurement can advance the adoption of complex innovations such as evidence-based practices.

  5. Measures for Predictors of Innovation Adoption

    OpenAIRE

    Chor, Ka Ho Brian; Wisdom, Jennifer P.; Olin, Su-Chin Serene; Hoagwood, Kimberly E; Horwitz, Sarah M.

    2015-01-01

    Building on a narrative synthesis of adoption theories by Wisdom et al. (2013), this review identifies 118 measures associated with the 27 adoption predictors in the synthesis. The distribution of measures is uneven across the predictors and predictors vary in modifiability. Multiple dimensions and definitions of predictors further complicate measurement efforts. For state policymakers and researchers, more effective and integrated measurement can advance the adoption of complex innovations s...

  6. How to Adopt a Child from China

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    Foreigners who want to adopt a child from China should present the documents listed below, through their government or an adoption organization entrusted by their government, to a Chinese government empowered adoption organization. These documents should be notarized by the foreign affairs office of their country, or an organization it so empowers, as well as by the Chinese embassy or consulate in their country. These documents include:

  7. Business model elements impacting cloud computing adoption

    DEFF Research Database (Denmark)

    Bogataj, Kristina; Pucihar, Andreja; Sudzina, Frantisek

    The paper presents a proposed research framework for identification of business model elements impacting Cloud Computing Adoption. We provide a definition of main Cloud Computing characteristics, discuss previous findings on factors impacting Cloud Computing Adoption, and investigate technology a...... blocks are investigated: Product Innovation, Infrastructure management, Customer relationship, Financial Aspects.......The paper presents a proposed research framework for identification of business model elements impacting Cloud Computing Adoption. We provide a definition of main Cloud Computing characteristics, discuss previous findings on factors impacting Cloud Computing Adoption, and investigate technology...

  8. A matter of attachment? How adoptive parents foster post-institutionalized children's social and emotional adjustment.

    Science.gov (United States)

    Barone, Lavinia; Lionetti, Francesca; Green, Jonathan

    2017-03-22

    The current study investigates the contribution of children's age at adoption (M = 46.52 months, SD = 11.52 months) and parents' attachment on post-institutionalized children's attachment and social-emotional adjustment. A total of 132 subjects, 48 post-institutionalized children aged 3-5 years, and their adoptive parents, took part in the study. One year from adoption, children's attachment distribution was as follows: 31% secure, 42% disorganized, and 27% insecure. Parents' secure attachment increased children's probability of presenting a secure attachment pattern; specifically, mothers' attachment patterns were most strongly associated with those of their adopted children, with fathers' making an additional contribution. Two years from adoption, secure children showed more adequate social competences than their insecure and disorganized peers and presented better emotional comprehension. The effect of age at adoption was delimited to a marginal association with behavioral problems. This pattern of associations suggests that attachment - both of adoptive parents and of children - substantially fosters social-emotional adjustment of post-institutionalized children who have experienced a period in emotionally neglecting environments beyond their first year of life, regardless of their age at adoption. Implications for policies and practices are discussed.

  9. Methodological findings in studies on adoptive families

    Directory of Open Access Journals (Sweden)

    Kalus, Alicja

    2014-09-01

    Full Text Available The issue of adoptive families is the object of interest for many researchers, especially outside Polish borders. A critical analysis of previous studies on adoption shows that the scientific value of many of the existing studies raises doubts. The reasons of this are more or less significant methodological weaknesses, which significantly reduce their scientific value. Researchers often focus on the study of individuals in the adoptive family, e.g. mothers only, adopted children only or selected types of relationships within the family e.g. the mother – child relation. There is a little amount of studies on family systems. It seems worthy to note that numerous studies are conducted in the cause-effect model of explaining phenomena in the adoptive family. Such approach to the study of reality, which is the family, is regarded as too big simplification. Therefore, there is an important reason to undertake the scientific discourse on the research methodology on the adoptive family. Discussed in the paper issues include: theoretical models, arrangement of studies, especially the selection of the test group and the research methods used in the study on adoptive families. Conducting scientific research is essential for the adoption practice. Among psychologists-practitioners exists a disturbing belief that working with adoptive families can be based on the experience gained in psychological practice. This fact can be explained by the existing lack of willingness of adoptive families to cooperate in the field of scientific research. Lack of this cooperation significantly limits the access to the study population of adoptive families.

  10. Predictors of race, adoption, and sexual orientation related socialization of adoptive parents of young children.

    Science.gov (United States)

    Goldberg, Abbie E; Smith, JuliAnna Z

    2016-04-01

    Using a sample of 125 lesbian, gay, and heterosexual adoptive parent couples with young children (M = 6.32 years), this study examined predictors of direct socialization (preparation for adoptism, racism, and heterosexism) and indirect socialization (modeling interactions by responding to outsiders' inquiries about their child's adoptive status, racial background, or family structure). In terms of direct socialization, parents of older children tended to engage in more socialization around adoptism and heterosexism, and parents of daughters tended to engage in more socialization around racism and heterosexism. Greater perceived child interest in adoption was related to more direct socialization around adoptism. Parents of color reported more direct socialization around racism. Having a child of color was related to more direct socialization around heterosexism. Regarding indirect socialization, sexual minority parents reported more socialization around adoption and race. Greater perceived child interest in adoption was related to more indirect adoption socialization. Being more "out" was related to more indirect socialization around parent sexual orientation.

  11. Characterization of Early and Late Adopters of ATM Card in Indian Banking Industry

    Directory of Open Access Journals (Sweden)

    Kamalpreet Kaur

    2012-10-01

    Full Text Available The present study deals with affect of adoption pattern of the ATMs by banks on its characteristics. With the exploration of various characteristics of the banks like Size, Profi tability, Efficiency, Cost of Operations, Asset quality and Credit risk, Financing Pattern, Diversifi cation and Age etc.; the study has tried to differentiate between the early and late adopter category of the banks regarding ATM cards. The banks have been categorized into early and late adopters on the basis of their adoption period. For this purpose, 50 scheduled commercial banks consisting of 27 Public Sector Banks and 23 Private Sector Banks have been taken as sample to investigate the various aspects of and early adopter banks in comparison to late adopter banks. The time period of the study is 20 years i.e. from 1991 to 2010. It can be concluded that the initiators and fi rst movers take advantage over the late adopters and laggards. They have found to perform better in terms of various parameters. Overall, the early adopter banks are larger in size, more diversifi ed, having lesser branches, more market share and wide ATM network as compared to late adopter ones. Thus, the empirical results evidently reveal that the both the groups have their own different characteristics.

  12. ICT as an Enabler for Innovation Adoption

    NARCIS (Netherlands)

    M. Verkerk (Michel); G.J. van der Pijl (Gert); E. van Asperen (Eelco)

    2009-01-01

    textabstractIn this paper we investigate how two online services (a portal and a digital library) may influence the adoption of an innovation. It is known from prior surveys that the installation services branch of the Dutch building industry has a relatively slow adoption rate for innovations. We e

  13. Mother-infant attachment in adoptive families.

    Science.gov (United States)

    Singer, L M; Brodzinsky, D M; Ramsay, D; Steir, M; Waters, E

    1985-12-01

    Data from 2 separate samples using the Strange Situation paradigm were combined to assess the quality of attachment relationships in adoptive and nonadoptive mother-infant pairs. Infants were between 13 and 18 months at the time of observation. Results indicated no differences in mother-infant attachment between nonadopted and intraracial adopted subjects or between intraracial and interracial adopted subjects. Interracial adoptive mother-infant pairs did show a higher incidence of insecure attachment in comparison to nonadoptive pairs. Mothers of interracial adopted infants also were less comfortable having others care for their babies and perceived less emotional support from extended family and friends for their decision to adopt a child prior to the actual adoption than did other mothers. No relation was found, however, between quality of mother-infant attachment and either perceived social support, infant developmental quotient, infant temperament, number of foster homes experienced by the infant, or infant's age at the time of adoption placement. It was suggested that the higher incidence of psychological problems found among adoptees in middle childhood and adolescence cannot be explained in terms of insecure attachment relationships during the infancy years.

  14. Euro adoption in Romania: Facts and perceptions

    Directory of Open Access Journals (Sweden)

    I.-C. Enache

    2013-06-01

    Full Text Available The aim of this paper is to link the nominal convergence criteria with Romanian citizens perceptions about euro adoption. Based on convergence reports and Euro-barometer data, the 2010 and 2012 years are analysed. It is shown that even if the nominal convergence achievements are improving, the perceptions regarding the euro adoption are deteriorating. A better communication strategy is advised.

  15. Adoption of communication technologies and national culture

    NARCIS (Netherlands)

    Hofstede, G.J.

    2001-01-01

    This paper investigates the question what attributes of countries influence the differential speed at which they adopt new communication technologies. On the basis of empirical data, it concludes that besides GNP per capita, cultural variables predict the speed of technology adoption. In particular,

  16. Intercountry Adoption Agencies and the HCIA

    NARCIS (Netherlands)

    P. Selman (Peter)

    2014-01-01

    textabstractThis report discusses concerns raised by participants of Thematic Area 3 (Intercountry Adoption Agencies and the HCIA) of the International Forum on Intercountry Adoption and Global Surrogacy held in August 2014. The aim is to report the views of those participating in this area on the i

  17. 32 CFR 584.4 - Adoption proceedings.

    Science.gov (United States)

    2010-07-01

    ... 32 National Defense 3 2010-07-01 2010-07-01 true Adoption proceedings. 584.4 Section 584.4 National Defense Department of Defense (Continued) DEPARTMENT OF THE ARMY PERSONNEL FAMILY SUPPORT, CHILD CUSTODY, AND PATERNITY § 584.4 Adoption proceedings. (a) General. This chapter does not apply to...

  18. Holistic BIM Adoption and Diffusion in Malaysia

    Directory of Open Access Journals (Sweden)

    Mamter Shahela

    2016-01-01

    Full Text Available Building Information Modeling is modeling technology and associated set of processes to produce, communicate and analyze digital information models for construction life cycle. Although many benefits can gain by the implementation of BIM, the pace adoption of BIM in Malaysia is still slow. There is an abundance of theories and models purporting to describe the drivers and mechanisms of how new technologies are adopted or diffused into a particular section of society or by individuals or stake players. In addition, many acceptance theories, which imply the adoption of new technologies and can be applied in every sector. This paper aims to present a review of the existing literature of BIM adoption and diffusion theory/model/framework in Malaysia. Researcher revealed the list of driven factors of Holistic BIM Adoption. In addition, it will deal with how to work effectively with groups that initially are relatively

  19. Relación entre el Parque de Tractores Agrícolas y el Patrón de Difusión y Adopción mediante un Modelo Logístico Relationship between the Agricultural Tractor Stock and the Adoption and Diffusion Patterns using a Logistic Model

    Directory of Open Access Journals (Sweden)

    Ricardo M Muñoz

    2011-01-01

    Full Text Available Este estudio determina el parque de tractores y la evolución del crecimiento de la adopción agregada del tractor agrícola en Chile. Se aplicó un modelo logístico para determinar el crecimiento de la adopción y la rapidez de adopción del tractor agrícola. Los parámetros del modelo se determinaron mediante un procedimiento no lineal. Se usó el balance del parque de tractores y la curva de tractorización y de adopción para estimar la variación de las unidades entre t y t-1 y se relacionó con la incorporación de tractores y con aquellos dados de baja. El modelo logístico mostró un nivel de ajuste significativo y permitió estimar la tasa de adopción y el nivel de saturación de la adopción. Se concluye que la metodología se puede aplicar para el estudio de la adopción de tecnologías en otras regiones y otros países.This paper determines the tractor stock and aggregate adoption growth of agricultural tractors in Chile. A logistic model was applied to determine the adoption growth and the adoption speed of agricultural tractors. Model parameters were determined by a non-linear algorithm. The tractor stock balance and the tractorization curve were used to estimate variation rate between t and t-1. This variation was associated with the introduction of tractors and those units removed from the stock. The logistic model showed a significant adjustment level and allowed estimating the rate of adoption and the adoption saturation level. It is concluded that the methodology can be applied to study technological adoption in other regions and other countries.

  20. Characterisation of adopters and non-adopters of dairy technologies in Ethiopia and Kenya.

    Science.gov (United States)

    Kebebe, E G; Oosting, S J; Baltenweck, I; Duncan, A J

    2017-04-01

    While there is a general consensus that using dairy technologies, such as improved breeds of dairy cows, can substantially increase farm productivity and income, adoption of such technologies has been generally low in developing countries. The underlying reasons for non-adoption of beneficial technologies in the dairy sector are not fully understood. In this study, we characterised adopters and non-adopters of dairy technologies in Ethiopia and Kenya based on farmers' resources ownership in order to identify why many farmers in Ethiopia and Kenya have not adopted improved dairy technologies. As compared to non-adopters, farmers who adopt dairy technology own relatively more farm resources. The result signals that differences in resource endowments could lead to divergent technology adoption scenarios. Results show that a higher proportion of sample smallholders in Kenya have adopted dairy technologies than those in Ethiopia. Except for the use of veterinary services, fewer than 10% of sample farmers in Ethiopia have adopted dairy technologies-less than half the number of adopters in Kenya. The higher level of dairy technology adoption in Kenya can be ascribed partly to the long history of dairy development, including improvements in the value chain for the delivery of inputs, services and fluid milk marketing. Interventions that deal with the constraints related to access to farm resources and input and output markets could facilitate uptake of dairy technology in developing countries.

  1. Korean Adoptee Identity: Adoptive and Ethnic Identity Profiles of Adopted Korean Americans

    Science.gov (United States)

    Beaupre, Adam J.; Reichwald, Reed; Zhou, Xiang; Raleigh, Elizabeth; Lee, Richard M.

    2015-01-01

    Adopted Korean adolescents face the task of grappling with their identity as Koreans and coming to terms with their adoptive status. In order to explore these dual identities, the authors conducted a person-centered study of the identity profiles of 189 adopted Korean American adolescents. Using cluster analytic procedures, the study examined…

  2. Cultural Tourism in Transnational Adoption: "Staged Authenticity" and Its Implications for Adopted Children

    Science.gov (United States)

    Quiroz, Pamela Anne

    2012-01-01

    The discursive practices of adoptive parents in two online transnational adoption forums (2006-2008) and observations of five international adoption workshops suggest that what Heather Jacobson described as "culture keeping", the cultural socialization of children that retains a sense of native group identity, is more aptly characterized as…

  3. Distributed PV Adoption in Maine Through 2021

    Energy Technology Data Exchange (ETDEWEB)

    Gagnon, Pieter; Sigrin, Ben

    2015-11-06

    NREL has used its dSolar (distributed solar) model to generate low-medium-high estimates of distributed PV adoption in Maine through 2021. This presentation gives a high-level overview of the model and modeling results.

  4. Improvisation during Process-Technology Adoption

    DEFF Research Database (Denmark)

    Tjørnehøj, Gitte; Mathiassen, Lars

    2010-01-01

    SPI technology adoption and events that causes the process to drift in unpredictable directions. To further understand how management's attempt to control the process is complemented by drifting, this article investigates the role of improvisation in adoption of SPI technology in a Danish software......Most software firms struggle to take advantage of the potential benefits of software process improvement (SPI) as they adopt this technology into the complex and dynamic realities of their day-to-day operation. Such efforts are therefore typically fluctuating between management's attempt to control...... in the adoption process despite constrained resources, and, most importantly, helped adapt SPI technology to the everyday practices at SmallSoft. However, we also identified un-called for improvisations and outcomes that were uncoordinated with SmallSoft's goals. Based on these findings we discuss how management...

  5. Adopt a Pixel Photographs: 2013-Present

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The photographs in the Adopt a Pixel collection were provided by volunteers with a digital camera, a Global Positioning System (GPS), and a compass or a smart mobile...

  6. 76 FR 81 - Adoption of Recommendation

    Science.gov (United States)

    2011-01-03

    ...; ] ADMINISTRATIVE CONFERENCE OF THE UNITED STATES Adoption of Recommendation AGENCY: Administrative Conference of... the attached recommendation at its Fifty-third Plenary Session. The recommendation addresses issues... makes recommendations for improvements to the agencies, collectively or individually, and to...

  7. Organizational Green IT Adoption: Concept and Evidence

    Directory of Open Access Journals (Sweden)

    Qi Deng

    2015-12-01

    Full Text Available Green IT has emerged as an important research topic in information systems and in other areas, such as business sustainability management. Some progress has been made in our understandings of green IT in a wide area of research topics, ranging from the green IT definition to the motivation for adopting green IT by organizations. This paper provides a holistic review and explanation of why organizations adopt green IT. Based on an extensive review of extant studies and a broad theoretical foundation, the paper presents a theoretical framework on organizational green IT adoption (OGITA. For researchers, the study provides a comprehensive review of previous green IT adoption studies and a roadmap for future research. For practitioners, the study provides managers and policy makers a systematic analytical framework in guiding their business decisions.

  8. Adopt a Pixel Photographs: 2013-Present

    Data.gov (United States)

    U.S. Geological Survey, Department of the Interior — The photographs in the Adopt a Pixel collection were provided by volunteers with a digital camera, a Global Positioning System (GPS), and a compass or a smart...

  9. Improvisation during Process-Technology Adoption

    DEFF Research Database (Denmark)

    Tjørnehøj, Gitte; Mathiassen, Lars

    2010-01-01

    SPI technology adoption and events that causes the process to drift in unpredictable directions. To further understand how management's attempt to control the process is complemented by drifting, this article investigates the role of improvisation in adoption of SPI technology in a Danish software...... firm, SmallSoft, over a 10-year period (1996-2005). We found that micro-level and macro-level improvisations interacted, often in uncoordinated ways, to shape SPI technology adoption at SmallSoft. The improvisations enhanced employee creativity, motivation and empowerment, created momentum...... in the adoption process despite constrained resources, and, most importantly, helped adapt SPI technology to the everyday practices at SmallSoft. However, we also identified un-called for improvisations and outcomes that were uncoordinated with SmallSoft's goals. Based on these findings we discuss how management...

  10. Methodology for Web Services Adoption Based on Technology Adoption Theory and Business Process Analyses

    Institute of Scientific and Technical Information of China (English)

    AN Liping; YAN Jianyuan; TONG Lingyun

    2008-01-01

    Web services use an emerging service-oriented architecture for distributed computing. Many organizations are either in the process of adopting web services technology or evaluating this option for incorporation into their enterprise information architectures. Implementation of this new technology requires careful assessment of the needs and capabilities of an organization to formulate adoption strategies. This paper presents a methodology for web services adoption based on technology adoption theory and business process analyses. The methodology suggests that strategies, business areas, and functions within an organization should be considered based on the existing organizational information technology status during the process of adopting web services to support the business needs and requirements.

  11. Studies based on the Danish Adoption Register

    DEFF Research Database (Denmark)

    Petersen, Liselotte; Sørensen, Thorkild I A

    2011-01-01

    INTRODUCTION: The genetic and familial environmental influences making up familial correlations in traits and familial aggregation of diseases may be disentangled by adoption studies. Thus, resemblance between the adoptee and the biological relatives indicates a contribution of the segregating gene....... RESEARCH TOPICS: To illustrate the adoption study approach in research, we present results achieved in schizophrenia, body fatness, smoking, and mortality. Significantly higher prevalence of schizophrenia and related conditions were found in biological relatives to adoptees with schizophrenia than among...

  12. Herd behaviour, strategic complementarities and technology adoption

    OpenAIRE

    2004-01-01

    In technology adoption, herd behaviour can lead to a suboptimal outcome. An example is given by Choi (1997): it is a model of technology choice under uncertainty where herding arises because of strategic complementarities and risk aversion. It causes a positive experimenting bias against the adoption of a more efficient (in terms of expected value) technology. We introduce in his model an additional element upon which firms base their technology decision: the economic environment. We investig...

  13. [adoption Of Standard Precautions: A Report].

    OpenAIRE

    2015-01-01

    During 1993 and 1994, a recycling program on Standard Precautions was conducted for the nursing personnel at the Women's Health Centre (CAISM). In 1995, an evaluation was obtained through direct observation of these sectors regarding the adoption of these measures, the adequate use, access and availability of gloves and boxes for the disposal of perforating, sharp material. Sixty observations and 232 procedures were reported. One hundred sixty four procedures (71%) adopted these measures and ...

  14. The Adoption and Diffusion of Service Products

    OpenAIRE

    Myung Joong Kwon

    2003-01-01

    The objective of this paper is to theoretically explore the innovation, adoption and diffusion of service product. A theoretical model of the diffusion of service product is developed that takes account of transportation, waiting and searching casts in the adoption of service product. The main results of the model are; (1) the diffusion of service product is slower than that of the manufacturing equivalent and (2) the delivery or retail distribution service speeds up the diffusion of the manu...

  15. The family romance fantasies of adopted children.

    Science.gov (United States)

    Wieder, H

    1977-01-01

    Despite the absence of psychoanalytic studies to support the position, family romance fantasies have been assumed to be similar in form and function in both the adopted child and the blood kin child. My studies, documented herein from the analyses of three adoptees, reveal that knowing one is adopted results in modifications of the fantasy and that certain types of adoptees seem incapable of effectively creating the paradigmatic family romance fantasy.

  16. Consumer Adoption Challenges To The Smart Grid

    Directory of Open Access Journals (Sweden)

    Anu Gupta

    2012-12-01

    Full Text Available The smart grid represents the next significant evolution of the power infrastructure with many enhancements and challenges. This paper provides a qualitative review of the key consumer adoption challenges of the smart grid. This literature review takes disparate pieces of work and identifies strategic research focal areas. Through existing research and media documents, it identifies the primary consumer adoption challenges as privacy, Radio Frequency (RF safety, and power rate increases. It also provides a review of each adoption challenge. The review is applied to the Canadian perspective drawing from smart grid experiences across North America. The review demonstrates that each of the challenge areas has a negative impact on the adoption and support of the smart grid. This paper recommends further in-depth research be conducted in the following areas: testing each of the consumer adoption challenges—privacy, RF Safety, and rate increases—separately with quantitative measures; testing the willingness to accept a power infrastructure with the security and stability level of a bank; and testing the impact that proactively educating the public would have on the smart grid adoption.

  17. Enhancing adoptive parenting: a randomized controlled trial.

    Science.gov (United States)

    Rushton, Alan; Monck, Elizabeth; Leese, Morven; McCrone, Paul; Sharac, Jessica

    2010-10-01

    The aim was to conduct a pragmatic randomized controlled trial (RCT) to evaluate two parenting programmes designed for adopters of children late placed from care. Adoptive parents, with children between 3 and 8 years who were screened to have serious behavioural problems early in the placement, participated in home-based, manualized, parenting programmes delivered by trained and supervised family social workers. The adopters who agreed to join the study were randomly allocated to one of two parenting interventions or to a "services as usual" group. Baseline, immediate post-intervention and six-month follow-ups were assessed using questionnaires and adopter interviews. No cases were lost to follow-up at any point and satisfaction was high with both parenting interventions. At the six-month follow-up, a significant difference (p parenting" in favour of the intervention group (Effect Size d = 0.7). Negative parenting approaches were reduced in the intervention group. However, no significant differences in child problems were found between the intervention groups and control group, adjusting for baseline scores. Costs analysis showed that a relatively modest investment in post-adoption support would be well spent in improving adopters' satisfaction with parenting in the intervention group compared to the routine service group.

  18. Lessons from silage adoption studies in Honduras

    Directory of Open Access Journals (Sweden)

    Christoph Reiber

    2013-12-01

    Full Text Available To date, silage adoption has been low in the tropics, particularly under smallholder conditions. Innovation and adoption processes of silage technologies were promoted in drought-constrained areas of Honduras using a flexible, site-specific and participatory research and extension approach. A total of about 250 farmers participated in training workshops and field days conducted in 13 locations. Smallholders successfully ensiled maize, sorghum and/or Pennisetum spp., mainly in heap and earth silos, while adoption of little bag silage (LBS was low. LBS proved useful as a demonstration, experimentation and learning tool. A ‘silage boom’ occurred in 5 locations, where favorable adoption conditions included the presence of demonstration farms and involvement of key innovators, lack of alternative dry season feeds, perceived benefits of silage feeding, a favorable milk market and both extension continuity and intensity. The lack of chopping equipment was the main reason for non-adoption by poor smallholders. The study showed that, when targeting production system needs and farmer demands, silage promotion can lead to significant adoption, including at smallholder level, in the tropics. This experience could contribute to an increase in effectiveness and sustainability of silage extension in similar situations elsewhere.

  19. Beyond preadoptive risk: The impact of adoptive family environment on adopted youth's psychosocial adjustment.

    Science.gov (United States)

    Ji, Juye; Brooks, Devon; Barth, Richard P; Kim, Hansung

    2010-07-01

    Adopted children often are exposed to preadoptive stressors--such as prenatal substance exposure, child maltreatment, and out-of-home placements--that increase their risks for psychosocial maladjustment. Psychosocial adjustment of adopted children emerges as the product of pre- and postadoptive factors. This study builds on previous research, which fails to simultaneously assess the influences of pre- and postadoptive factors, by examining the impact of adoptive family sense of coherence on adoptee's psychosocial adjustment beyond the effects of preadoptive risks. Using a sample of adoptive families (n = 385) taking part in the California Long Range Adoption Study, structural equation modeling analyses were performed. Results indicate a significant impact of family sense of coherence on adoptees' psychosocial adjustment and a considerably less significant role of preadoptive risks. The findings suggest the importance of assessing adoptive family's ability to respond to stress and of helping families to build and maintain their capacity to cope with stress despite the sometimes fractious pressures of adoption.

  20. The Practice of Adoption: History, Trends, and Social Context

    Science.gov (United States)

    Zamostny, Kathy P.; O'Brien, Karen M.; Baden, Amanda L.; Wiley, Mary O'Leary

    2003-01-01

    This article presents an overview of the practice of adoption to counseling psychologists to promote clinical understanding of the adoption experience and to stimulate research on adoption. The article includes definitions of adoption terminology, important historical and legal developments for adoption, a summary of adoption statistics,…

  1. Moses and Superman Come Home: Counseling Adoptees and Adoptive Families.

    Science.gov (United States)

    Saiz, Stephen G.

    This paper looks at three parties impacted by adoption: the adoptive parents, the adopted child, and the adoptive family. When working with adoptive parents, counselors should respect the strength of the couple, their commitment to parenthood, and the closeness that may develop from weathering the issue of childlessness. Adoptive parents are…

  2. Parent adjustment over time in gay, lesbian, and heterosexual parent families adopting from foster care.

    Science.gov (United States)

    Lavner, Justin A; Waterman, Jill; Peplau, Letitia Anne

    2014-01-01

    Although increasing numbers of gay and lesbian individuals and couples are adopting children, gay men and lesbian women continue to face increased scrutiny and legal obstacles from the child welfare system. To date, little research has compared the experiences of gay or lesbian and heterosexual adoptive parents over time, limiting conceptual understandings of the similarities they share and the unique challenges that gay and lesbian adoptive parents may face. This study compared the adoption satisfaction, depressive symptoms, parenting stress, and social support at 2, 12, and 24 months postplacement of 82 parents (60 heterosexual, 15 gay, 7 lesbian) adopting children from foster care in Los Angeles County. Few differences were found between heterosexual and gay or lesbian parents at any of the assessments or in their patterns of change over time. On average, parents in both household types reported significant increases in adoption satisfaction and maintained low, nonclinical levels of depressive symptoms and parenting stress over time. Across all family types, greater parenting stress was associated with more depressive symptoms and lower adoption satisfaction. Results indicated many similarities between gay or lesbian and heterosexual adoptive parents, and highlight a need for services to support adoptive parents throughout the transition to parenthood to promote their well-being. (PsycINFO Database Record (c) 2014 APA, all rights reserved).

  3. Executive function and mental health in adopted children with a history of recreational drug exposures.

    Directory of Open Access Journals (Sweden)

    Brian J Piper

    Full Text Available Adoptive children are at increased risk for problematic behaviors but the origin of these individual differences in neurobehavioral function is unclear. This investigation examined whether adopted children with prenatal exposure to a wide variety of recreational drugs exhibited higher scores (i.e. more problems with executive function and psychiatric symptomology. Caregivers of children ages 5 to 18 completed an online survey with items about use of alcohol, nicotine, or methamphetamine during pregnancy followed by the Behavior Rating Inventory of Executive Function (BRIEF, N = 437 including 59 adoptive parents or the Child Behavior Checklist (CBCL, N = 549 including 54 adoptive parents. Relative to a comparison group of children raised by their biological parents, adoptive children that were polysubstance exposed during prenatal development exhibited higher rates of academic difficulties and were behind their classmates in math and reading. Adoptive children had statistically and clinically significant higher BRIEF ratings and this pattern was similar for boys and girls. CBCL ratings were significantly increased in adoptive children, particularly for Externalizing and Attention problems. Adoptive children with a history of polysubstance exposures including alcohol, nicotine, and methamphetamine are at heightened risk for difficulties with executive function as well as various psychopathologies. These findings suggest that increased monitoring to identify and implement remediation strategies may be warranted for adopted children with a history of in utero drug exposures.

  4. Adoption of Internet Shopping: Cultural Considerations in India and Australia

    Directory of Open Access Journals (Sweden)

    Sujana Adapa

    2008-08-01

    Full Text Available The current research paper examines the adoption of internet shopping patterns exhibited by Indian women currently residing in India and Australia emphasizing on the prevailing cultural dimensions. A conceptual framework has been developed based on the theoretical background which links intention to shop over internet and Hofstede’s cultural dimensions to adoption of internet shopping. In order to test the stated hypotheses, the proposed relationships between the variables were empirically verified. A web based survey was employed by using online questionnaire as a research instrument and the respondents were approached by posting the questionnaire to various newsgroups. The results of the study reveal that intention of int ernet shopping as measured with the perceived attributes significantly influences the actual adoption of internet shopping. With regard to the prevailing cultural dimensions in the country of origin (India, the results obtained are as expected and signifi cantly influence the internet purchases. Where as with regard to the prevailing cultural dimensions in the country of residence (Australia, most of the results obtained are as predicted except for the dimension masculinity versus femininity.

  5. Dermatologic conditions in internationally adopted children

    Directory of Open Access Journals (Sweden)

    Diane L. Whitaker-Worth, MD

    2015-02-01

    Full Text Available Over 200,000 children have been adopted into United States (US families from abroad since the year 2000. Health care providers who care for children adopted internationally should be aware of the spectrum of illnesses seen in this population, and should be prepared to encounter potentially unusual situations. An appreciation for the unique pre-adoption exposures and vulnerabilities inherent in international adoption is critical for proper diagnosis and treatment of this heterogeneous group of children. It is important to consider the impact of potential early childhood stressors such as nutritional, sensory, and emotional deprivation, trauma and abuse, as well as prenatal exposures to drugs, alcohol, and infectious diseases. Providers must also take into account international variation in health care practices, including immunization, treatment, surgical, and hygiene standards. The differential diagnosis for cutaneous eruptions in children adopted internationally is broad and must encompass endemic systemic illnesses with skin manifestations, such as measles, tuberculosis, leprosy, and congenital syphilis, and primary dermatologic diseases such as scabies and bacterial and fungal infections. The importance of maintaining a broad differential and open mind when addressing the dermatologic needs of these children cannot be overemphasized.

  6. The Cloud Adoption Toolkit: Addressing the Challenges of Cloud Adoption in Enterprise

    CERN Document Server

    Greenwood, David; Smith, James; Sommerville, Ian

    2010-01-01

    Cloud computing promises a radical shift in the provisioning of computing resource within enterprise. This paper: i) describes the challenges that decision-makers face when attempting to determine the feasibility of the adoption of cloud computing in their organisations; ii) illustrates a lack of existing work to address the feasibility challenges of cloud adoption in enterprise; iii) introduces the Cloud Adoption Toolkit that provides a framework to support decision-makers in identifying their concerns, and matching these concerns to appropriate tools/techniques that can be used to address them. The paper adopts a position paper methodology such that case study evidence is provided, where available, to support claims. We conclude that the Cloud Adoption Toolkit, whilst still under development, shows signs that it is a useful tool for decision-makers as it helps address the feasibility challenges of cloud adoption in enterprise.

  7. Adoption and non-adoption of mainstream formal banking systems amongst low income earners in the South Africa, Zambia and Zimbabwe

    Directory of Open Access Journals (Sweden)

    Mavhungu Abel Mafukata

    2015-03-01

    Full Text Available The majority of income earners of small-scale informal economic sectors in most developing regions of the world do not bank their earnings with mainstream formal banking institutions preferring to “bank” their income at their homes or not to bank at all. In addition, there is perception amongst mainstream formal banking institutions that low income earners were “unbankable” and therefore bad business. However, there is emerging literature opining that to the contrary, low income earners would provide a profitable niche market for mainstream formal banking institutions in the developing regions. In the Southern African Development Community (SADC, trends with regard adoption and non-adoption of mainstream formal banking systems amongst small income groups were mixed. This paper investigates adoption and non-adoption patterns of mainstream formal banking systems amongst small income earners in South Africa, Zambia and Zimbabwe. The results revealed that the informal cross-border traders who trade between Zambia and South Africa were good adopters of mainstream formal banking because the majority of them in these countries had already adopted mainstream formal banking through savings while only a few was left out of the system. However, there was a sharp contrast in Zambia where small income earners from the micro-finance adopting households were poorly banked with a huge majority which has been left out of the system. In Nyanga, Zimbabwe, of the respondents, only a few had adopted mainstream formal banking against the larger majority of non-adopters. The small income earners of the small-scale informal communal cattle farmers sub-sector in South Africa had 47.2% of its population adopting mainstream formal banking through savings whereas the majority 52.8% was left out. This paper concludes that adoption or non-adoption of mainstream formal banking systems patterns vary from region to region, and sector to sector even where income earners

  8. Breaking the addiction to technology adoption.

    Science.gov (United States)

    Bryan, Stirling; Mitton, Craig; Donaldson, Cam

    2014-04-01

    A major driver of cost growth in health care is the rapid increase in the utilisation of existing technology and not simply the adoption of new technology. Health economists and their health technology assessment colleagues have become obsessed by technology adoption questions and have largely ignored 'technology management' questions. Technology management would include the life-cycle assessment of technologies in use, to assess their real-world performance; and monitoring of technology indication creep. A rebalancing of focus might serve to encourage a more self-critical and learning culture amongst those involved in technology evaluation analysis. Further, health economists and health technology assessment analysts could make a more significant contribution to system efficiency through rebalancing their efforts away from technology adoption questions towards technology management issues.

  9. The Consumer Health Information System Adoption Model.

    Science.gov (United States)

    Monkman, Helen; Kushniruk, Andre W

    2015-01-01

    Derived from overlapping concepts in consumer health, a consumer health information system refers to any of the broad range of applications, tools, and educational resources developed to empower consumers with knowledge, techniques, and strategies, to manage their own health. As consumer health information systems become increasingly popular, it is important to explore the factors that impact their adoption and success. Accumulating evidence indicates a relationship between usability and consumers' eHealth Literacy skills and the demands consumer HISs place on their skills. Here, we present a new model called the Consumer Health Information System Adoption Model, which depicts both consumer eHealth literacy skills and system demands on eHealth literacy as moderators with the potential to affect the strength of relationship between usefulness and usability (predictors of usage) and adoption, value, and successful use (actual usage outcomes). Strategies for aligning these two moderating factors are described.

  10. Role of value compatibility in IT adoption

    DEFF Research Database (Denmark)

    Bunker, Deborah; Kautz, Karlheinz; Nguyen, Anne Luu Thanh

    2007-01-01

    Compatibility has been recognised as an important element in the adoption of IT innovations in organisations but as a concept it has been generally limited to technical or functional factors. Compatibility is also significant, however, with regard to value compatibility between the organisation......, and the adopted IT innovation. We propose a framework to determine value compatibility analysing the organisation's and information system's structure, practices and culture, and explore the value compatibility of an organisation with its adopted self-service computer-based information system. A case study...... was conducted to determine the congruence of an organisation's value and IT value compatibility. This study found that there was a high correspondence in the organisational structure and practice dimensions; however, there were organisational culture disparities. The cultural disparities reflected the self...

  11. Technology adoption in nonrenewable resource management

    Energy Technology Data Exchange (ETDEWEB)

    Cunha-e-Sa, Maria A.; Balcao Reis, Ana [Universidade Nova de Lisboa, Faculdade de Economia, Campus de Campolide, PT-1099-032, Lisboa (Portugal); Roseta-Palma, Catarina [Departamento de Economia, ISCTE, Avenida das Forcas Armadas, PT-1649-026, Lisboa (Portugal)

    2009-03-15

    Technological change has played an important role in models of nonrenewable resource management, since its presence mitigates the depletion effect on extraction costs over time. We formalize the problem of a competitive nonrenewable resource extracting firm faced with the possibility of technology adoption. Based on a quadratic extraction cost function, our results show that the expected net benefits from adoption increase both with the size of the resource stock and with prices. A boundary that separates the region where expected net benefits are positive from the one where they are negative is derived. (author)

  12. [Adoption of standard precautions: a report].

    Science.gov (United States)

    Lopes, M H; Moromizato, S S; Veiga, J F

    1999-10-01

    During 1993 and 1994, a recycling program on Standard Precautions was conducted for the nursing personnel at the Women's Health Centre (CAISM). In 1995, an evaluation was obtained through direct observation of these sectors regarding the adoption of these measures, the adequate use, access and availability of gloves and boxes for the disposal of perforating, sharp material. Sixty observations and 232 procedures were reported. One hundred sixty four procedures (71%) adopted these measures and 68 procedures (29%) did not. It was evident that the need to wash hands and not to use needles twice should be emphasized.

  13. Adopting Deverbalization in Interpreting Chinese Political Neologisms

    Institute of Scientific and Technical Information of China (English)

    计霄雯

    2014-01-01

    Uniqueness of our political systems and tremendous changes brought about by reform and opening up are reflected in language use, so a boom of Chinese political neologisms is witnessed. Deverbalization, as the core concept of the Interpretive Theory should be adopted in interpreting such neologisms so as to convey the intended meaning of the speaker in a faithful and interpretive manner. This paper makes a tentative attempt at classifying those neologisms and elaborates through exemplification on adopting deverbalization in interpreting political neologisms, with a view to producing China English rather than Chinese English and facilitating political communication among countries.

  14. A Paradigm Shift: Adoption of Disruptive Learning Innovations in an ODL Environment: The Case of the University of South Africa

    Science.gov (United States)

    Mbatha, Blessing

    2015-01-01

    The aim of this article is to shed some light on patterns of and major motives for the adoption of different types of disruptive learning innovations by Unisa academics. To realise the aim of the study, the following questions were addressed: What are the reasons for adopting disruptive learning innovations? What is the level of interaction with…

  15. Inter-Organizational Information Systems Adoption

    DEFF Research Database (Denmark)

    Lyytinen, Kalle; Damsgaard, Jan

    2011-01-01

    In this article we propose a new complementary approach to investigate Inter-Organizational Information Systems (IOIS) adoption called configuration analysis. We motivate the need for a new approach by the common observation that the structure and the strategy of an IOIS are interdependent...

  16. Distributed PV Adoption - Sensitivity to Market Factors

    Energy Technology Data Exchange (ETDEWEB)

    Gagnon, Pieter; Sigrin, Ben

    2016-02-01

    NREL staff used the dSolar (distributed solar) model to forecast the adoption of distributed, behind-the-meter PV through the year 2050 for 9 different scenarios. The scenarios varied in their assumptions about a carbon tax, the cost of PV systems in the future, and what credit would be given for excess generation once current net metering policies expire.

  17. Adoption of Energy Conservation among California Homeowners.

    Science.gov (United States)

    Leonard-Barton, Dorothy; Rogers, Everett M.

    In spring 1977, just as California was emerging from one of the worst droughts in its history, 215 Palo Alto homeowners were interviewed about their views on energy and water conservation, and about the extent to which they had adopted 11 energy-conserving practices (ECP) in the home. The objective was to discover variables both important to…

  18. Adoption of Precision farming in Denmark

    DEFF Research Database (Denmark)

    Pedersen, Søren Marcus; Fountas, S.; Blackmore, S.

    2003-01-01

    The adoption of precision farming (PF)in Denmark has been studied through two mail surveys, personal interviews and focus groups with farmers and other stakeholders. Farmers are in general optimistic about PF but it is difficult to verify the economic and environmental gains. It has been difficult...

  19. Overseas Adoptions in the U.S.

    Institute of Scientific and Technical Information of China (English)

    JerilynWatson; 刘保行

    2004-01-01

    Every year, thousands of Americans take a child into their home that was born in another country. Many husbands and wives in the USA want a child, but may not be able to have one. Or, such married couples may want to add an adopted (收养的) child to their other children. Single Americans may also want to become parents.

  20. Negotiating the Dance: Consulting with Adoptive Systems.

    Science.gov (United States)

    Becker, Kent W.; Carson, David K.; Seto, Atsuko; Becker, Carol A.

    2002-01-01

    For marriage and family therapists and educators who train future marriage and family therapists, families formed by means of adoption offer an abundance of learning opportunities in both the areas of assessment and intervention. The following consultation case represents a composite family designed to highlight the unique features of adoptive…

  1. Outcomes of Children Adopted from Eastern Europe

    Science.gov (United States)

    Miller, Laurie; Chan, Wilma; Tirella, Linda; Perrin, Ellen

    2009-01-01

    Behavioral problems are frequent among post-institutionalized Eastern European adoptees. However, risk factors related to outcomes have not been fully delineated. We evaluated 50 Eastern European adoptees, age 8-10 years, with their adoptive families for more than five years. Cognitive and behavioral outcomes and parenting stress were evaluated in…

  2. Effects of the history of adoption in the emotional adjustment of adopted adolescents.

    Science.gov (United States)

    Reppold, Caroline Tozzi; Hutz, Claudio Simon

    2009-11-01

    Since the decade of 1980, the model of stress and coping proposed for the assessment of vulnerability of adoptive families emphasizes that the emotional adjustment of those adopted is moderated by variables such as institutionalization, the manner and age at which the adoption was revealed, the change of first name, and the contact with the biological family. The objective of this study was to investigate the relationship of these variables to the perceived parenting style, mood, and self-esteem of the adopted adolescents. Participants in the study were 68 adolescents, between the ages of 14 and 15, adopted during infancy through judicial channels. The adolescents responded to a questionnaire about the history of adoption and to scales of Parenting Styles, Depression and Self-esteem. The main results indicated that the late revelation of adoption and the change of the first name are connected to higher levels of depression and low self-esteem and to more frequent perceptions of negligent or authoritarian parenting style. The contact with the biological family was frequently mentioned among those who perceived their parents as authoritative and presented the best indicator of mood and self-esteem. These findings were discussed in light of the necessity for multidisciplinary actions which can improve the psychological adaptation of the adopting families.

  3. The Social Construction of Adoption: Clinical and Community Perspectives.

    Science.gov (United States)

    Miall, Charlene E.

    1996-01-01

    Discusses the social construction of adoptive parenting and adopted children as reflected in clinical and adoption triangle research. Although clinicians view adoptive families as second best to biologically related families, a random sample of 150 respondents did not differentiate between the functioning of adoptive and biologically related…

  4. Active Drivers of Adoption of Internet Banking

    Directory of Open Access Journals (Sweden)

    Pankaj Yadav

    2016-05-01

    Full Text Available Application of internet by banking sector in India has changed the definition of banking completely. The management of different banks has been making concrete efforts to facilitate the acceptance of i-banking by bringing out its benefits to the customers. This paper identifies the active factors that influence customers intention regarding use of internet banking in Himachal Pradesh  Data was collected from a sample of 120 respondents through a well structured questionnaire. The data was collected from Government employees of Shimla district of Himachal Pradesh by asking close ended questions regarding their attitude towards i-banking and their future intention about its use . For analysing and interpreting data in the present study, basic statistical tools, chi-square test (test of independence and logistical regression analysis was used with the help of SPSS 16.0 version. Out of seven factors i.e., perceived risk, perceived usefulness,  perceived quality of service, Perceived cost, Perceived ease of use, trust and awareness, three significant factors i.e., perceived risk, perceived usefulness  and perceived quality of service have been identified that actively influence future adoption intention to adopt internet banking . Further the study exhibited that perceived usefulness and perceived quality of service positively influence the intention to adopt i-banking while perceived risk negatively affect the future adoption intention of i-banking. This  model has the overall predictability of classifying 76.7 % cases correctly and exhibited that 87.2 % customers are classified for adopting i-banking service whereas 57.1 % customers for not having intention to use it in future

  5. A Disciplined Approach to Adopting Agile Practices: The Agile Adoption Framework

    CERN Document Server

    Sidky, Ahmed; Bohner, Shawn

    2007-01-01

    Many organizations aspire to adopt agile processes to take advantage of the numerous benefits that it offers to an organization. Those benefits include, but are not limited to, quicker return on investment, better software quality, and higher customer satisfaction. To date however, there is no structured process (at least in the public domain) that guides organizations in adopting agile practices. To address this problem we present the Agile Adoption Framework. The framework consists of two components: an agile measurement index, and a 4-Stage process, that together guide and assist the agile adoption efforts of organizations. More specifically, the agile measurement index is used to identify the agile potential of projects and organizations. The 4-Stage process, on the other hand, helps determine (a) whether or not organizations are ready for agile adoption, and (b) guided by their potential, what set of agile practices can and should be introduced.

  6. 用等位基因特异性PCR/限制性片段长度多态性策略构建原发性青光眼致病基因CYP1B1的单倍型%Construction of CYP1B1 gene haplotypes predisposing to primary congenital glaucoma through allele-specific PCR/restriction fragment length polymorphism analysis

    Institute of Scientific and Technical Information of China (English)

    张爱平; 李圣杰; 欧阳琦; 汤荔; 王晓蕾; 吉建; 曹文俊

    2015-01-01

    目的 建立等位基因特异性PCR/限制性片段长度多态性(allele-specific PCR/restriction fragment length polymorphism,AS-PCR/RFLP)法检测原发性先天性青光眼(primary congenital glaucoma,PCG)致病基因CYP1B1常见单核苷酸多态性(single nucleotide polymorphisms,SNPs)及其单倍型的方法.方法 收集20例原发性先天性青光眼患者和20名正常对照为研究对象,首先经测序筛查SNP位点后,再分别以PCR-RFLP和AS-PCR/RFLP策略构建CYP1B1基因rs10012(S1)和rs1056827(S2)及rs1056836(S3)和rs1056837(S4)位点的单倍型,并对这两种策略进行评价.结果 测序共发现4个SNP位点,为第2外显子rs10012 G/C(S1)及rs1056827 T/G (S2)、第3外显子rs1056836C/G(S3)及rs1056837T/C(S4).这些位点在PCG患者和正常对照中的分布呈现不同特点,同时存在rs10012 (S1)和rs1056827(S2)位点的PCG患者和正常对照分别为10例(50%)和5人(25%);同时存在rs1056836 (S3)和rs1056837(S4)位点的PCG患者和正常对照分别为5例(25%)和2人(10%);均未发现上述SNP位点单独存在.确定各位点的分布特点后,首先用PCR-RFLP策略构建rs10012(S1)和rs1056827(S2)位点单倍型,显示杂合突变型除出现目的条带外还存在底物条带,虽提示同时存在rs10012(S1)和rs1056827 (S2),但仍不能证实存在位点间的连锁;AS-PCR/RFLP构建的结果显示,AS-PCR扩增rs10012(S1)位点获得阳性结果的同时,针对rs1056827 (S2)位点的特异性RFLP分析也获得阳性结果.AS-PCR/RFLP对位点rs1056836(S3)和rs1056837 (S4)的分析获得了类似的结果.应用AS-PCR/RFLP成功构建了C-G[rs10012(S1)-rs1056827(S2)]和G-C[rs1056836 (S3)-rs1056837 (S4)]两种单倍型.结论 应用AS-PCR/RFLP策略成功构建PCG致病基因CYP1B1单倍型,该方法准确高效特异可用于构建遗传性疾病基因的单倍型.%Objective To develop an allele-specific PCR (AS-PCR)/restriction fragment length polymorphism (RFLP) assay for CYP1B1 gene haplotypes

  7. Evaluating IPv6 Adoption in the Internet

    Science.gov (United States)

    Colitti, Lorenzo; Gunderson, Steinar H.; Kline, Erik; Refice, Tiziana

    As IPv4 address space approaches exhaustion, large networks are deploying IPv6 or preparing for deployment. However, there is little data available about the quantity and quality of IPv6 connectivity. We describe a methodology to measure IPv6 adoption from the perspective of a Web site operator and to evaluate the impact that adding IPv6 to a Web site will have on its users. We apply our methodology to the Google Web site and present results collected over the last year. Our data show that IPv6 adoption, while growing significantly, is still low, varies considerably by country, and is heavily influenced by a small number of large deployments. We find that native IPv6 latency is comparable to IPv4 and provide statistics on IPv6 transition mechanisms used.

  8. Treaty to Curb Mercury Pollution Adopted

    Science.gov (United States)

    Showstack, Randy

    2013-10-01

    The international Minamata Convention on Mercury to protect human health and the environment from anthropogenic emissions and releases of mercury and mercury compounds was formally adopted at a 10 October meeting in Minamata, Japan. The legally binding treaty, currently signed by 92 countries, comes 57 years after the government of Japan officially acknowledged, in 1956, the existence of Minamata disease, which was caused by eating seafood contaminated with methylmercury compounds discharged into Minamata Bay in southern Japan.

  9. Understanding Physicians' Adoption of Health Clouds

    Directory of Open Access Journals (Sweden)

    Tatiana Ermakova

    2015-01-01

    Full Text Available Recently proposed health applications are able to e nforce essential advancements in the healthcare sector. The design of these innovative s olutions is often enabled through the cloud computing model. With regards to this technology, h igh concerns about information security and privacy are common in practice. These concerns with respect to sensitive medical information could be a hurdle to successful adoptio n and consumption of cloud-based health services, despite high expectations and interest in these services. This research attempts to understand behavioural intentions of healthcare pro fessionals to adopt health clouds in their clinical practice. Based on different established t heories on IT adoption and further related theoretical insights, we develop a research model a nd a corresponding instrument to test the proposed research model using the partial least squ ares (PLS approach. We suppose that healthcare professionals’ adoption intentions with regards to health clouds will be formed by their outweighing two conflicting beliefs which are performance expectancy and medical information security and privacy concerns associate d with the usage of health clouds. We further suppose that security and privacy concerns can be explained through perceived risks.

  10. THE FORESIGHTS OF THE ADOPTION OF EURO

    Directory of Open Access Journals (Sweden)

    Claudia Loredana Juncu

    2012-06-01

    Full Text Available Even if the Euro critics showed the warning that the change of the national currency brings a loose of the control and the decision power about the monetary problems and the flexibility of the decisions, many consider that the adoption of the single currency would allow us to protect the economies from the external shocks. We have to eliminate the uncertainty, the high costs and we have to be able to avoid and / or manage the monetary fluctuations especially in situations like today’s economic crisis. One of the effects the crisis has created throughout the European Union and especially in Romania is connected with the return back in the country of the population that works in Western Europe and the reflux of money it implies. The structural problems of the Romanian economy that creates this workforce dynamic is to continue to affect the Euro adoption plan until significant solutions will be addressed in order to maintain the workforce in the country.Europe is facing a dilemma. For now there is a significant distrust in Euro. Romania needs to acknowledge the fact that a single European currency allows first of all the overpass of the actual internal fragmentation of the market and so to strengthen the national position in relation with the international. Romania can still be optimistic about the objective of adopting Euro if an efficient financial policy will be implemented.

  11. Trust: A Requirement for Cloud Technology Adoption

    Directory of Open Access Journals (Sweden)

    Akinwale O. Akinwunmi

    2015-08-01

    Full Text Available Cloud computing is a recent model for enabling convenient, on-demand network access to a shared pool of configurable computing resources such as networks, servers, storage, applications, and services; that can be rapidly provisioned and released with minimal management effort or service provider interaction. Studies have shown that cloud computing has the potential to benefit establishments, industries, national and international economies. Despite the enormous benefits cloud computing technology has the potentials of offering, several issues are making intended users to pause in adopting the usage of the technology. Users need to be assured of the safety and reliability of the technology while using it. This is needed to build confidence around the technology and reduce the level of anxiety. This research attempts to investigate the effect of trust in the adoption of the technology by formulating a trust model based on Expectancy Disconfirmation Theory model and Bayesian network. A simulation experiment was carried out to determine the significance of trust in the adoption of cloud technology.

  12. SMS advertisements\\\\\\' adoption among Tehran mobile users

    Directory of Open Access Journals (Sweden)

    Manizheh Haghighinasab

    2014-05-01

    Full Text Available Mobile marketing and SMS (short message services advertising in particular, have been recently used. Even though SMS-based advertising is inexpensive, interactive, and able to yield a high response rate, middle or big size companies do not use it in Iran. For as much as its adoption was unclear, this study was organized to find it out. Since the most populated city in our country, Tehran, is a good multicultural instance to study in many fields, this research has aimed at its citizens' intention to use SMS advertisement. The statistical populations included are mobile users, who came to Mobile Customer Service located in north, south, west, east and centre of Tehran. From which 384 were considered as samples. Technology Acceptance Model (TAM was used for making hypothesizes. Survey's data was collected by questionnaire with cluster and random sampling. Collected data was analyzed regarding to the method: Structural Equation Modeling (SEM in order to do which, the technique Partial List Squares (PLS were used. The results revealed that: despite the lack of adoption for the SMS advertising among mobile users in Tehran, there could be chances to improve it by better perceived usefulness as well as its ease of use. Innovativeness of the people and their trust has indirect impact on their intention to use. Moreover, getting prior permission, making a profile, and individualizing the context with richness of information and entertainers are recommended. Using the new generation 3G and shortcuts will be able to improve SMS advertisement's adoption as well.

  13. Pattern matching

    NARCIS (Netherlands)

    A. Hak (Tony); J. Dul (Jan)

    2009-01-01

    textabstractPattern matching is comparing two patterns in order to determine whether they match (i.e., that they are the same) or do not match (i.e., that they differ). Pattern matching is the core procedure of theory-testing with cases. Testing consists of matching an “observed pattern” (a pattern

  14. Cholesterol and male fertility: what about orphans and adopted?

    Science.gov (United States)

    Maqdasy, Salwan; Baptissart, Marine; Vega, Aurélie; Baron, Silvère; Lobaccaro, Jean-Marc A; Volle, David H

    2013-04-10

    The link between cholesterol homeostasis and male fertility has been clearly suggested in patients who suffer from hyperlipidemia and metabolic syndrome. This has been confirmed by the generation of several transgenic mouse models or in animals fed with high cholesterol diet. Next to the alteration of the endocrine signaling pathways through steroid receptors (androgen and estrogen receptors); "orphan" and "adopted" nuclear receptors, such as the Liver X Receptors (LXRs), the Proliferating Peroxisomal Activated Receptors (PPARs) or the Liver Receptor Homolog-1 (LRH-1), have been involved in this cross-talk. These transcription factors show distinct expression patterns in the male genital tract, explaining the large panel of phenotypes observed in transgenic male mice and highlighting the importance of lipid homesostasis and the complexity of the molecular pathways involved. Increasing our knowledge of the roles of these nuclear receptors in male germ cell differentiation could help in proposing new approaches to either treat infertile men or define new strategies for contraception.

  15. Comforting an orphaned nation : Representations of international adoption and adopted Koreans in Korean popular culture

    OpenAIRE

    Hübinette, Tobias

    2005-01-01

    This is a study of popular cultural representations of international adoption and adopted Koreans in Western countries. The study is carried out from a postcolonial perspective and uses a cultural studies reading of four feature films and four popular songs as primary sources. The aim is to examine how nationalism is articulated in various ways in light of the colonial experiences in modern Korean history and recent postcolonial developments within contemporary Korean society. The principal q...

  16. Enabling international adoption of LOINC through translation

    Science.gov (United States)

    Vreeman, Daniel J.; Chiaravalloti, Maria Teresa; Hook, John; McDonald, Clement J.

    2012-01-01

    Interoperable health information exchange depends on adoption of terminology standards, but international use of such standards can be challenging because of language differences between local concept names and the standard terminology. To address this important barrier, we describe the evolution of an efficient process for constructing translations of LOINC terms names, the foreign language functions in RELMA, and the current state of translations in LOINC. We also present the development of the Italian translation to illustrate how translation is enabling adoption in international contexts. We built a tool that finds the unique list of LOINC Parts that make up a given set of LOINC terms. This list enables translation of smaller pieces like the core component “hepatitis c virus” separately from all the suffixes that could appear with it, such “Ab.IgG”, “DNA”, and “RNA”. We built another tool that generates a translation of a full LOINC name from all of these atomic pieces. As of version 2.36 (June 2011), LOINC terms have been translated into 9 languages from 15 linguistic variants other than its native English. The five largest linguistic variants have all used the Part-based translation mechanism. However, even with efficient tools and processes, translation of standard terminology is a complex undertaking. Two of the prominent linguistic challenges that translators have faced include: the approach to handling acronyms and abbreviations, and the differences in linguistic syntax (e.g. word order) between languages. LOINC’s open and customizable approach has enabled many different groups to create translations that met their needs and matched their resources. Distributing the standard and its many language translations at no cost worldwide accelerates LOINC adoption globally, and is an important enabler of interoperable health information exchange PMID:22285984

  17. Will Your Household Adopt Your New Robot?

    OpenAIRE

    Bauwens, Valérie; Fink, Julia

    2012-01-01

    Domestic robots have slowly found their way into some of our homes and onto the shelves of major stores selling technical appliances. Who hasn’t already seen or heard of robots that vacuum or mow the lawn? As researchers in robotics, we feel this growing commercial success is a great opportunity to learn about robot adoption processes. Leaving the marketing buzz and usual fantasies about robot invasions aside, we are curious to find out how robots are perceived by users. Are robots revolution...

  18. Classification system adopted for fixed cutter bits

    Energy Technology Data Exchange (ETDEWEB)

    Winters, W.J.; Doiron, H.H.

    1988-01-01

    The drilling industry has begun adopting the 1987 International Association of Drilling Contractors' (IADC) method for classifying fixed cutter drill bits. By studying the classification codes on bit records and properly applying the new IADC fixed cutter dull grading system to recently run bits, the end-user should be able to improve the selection and usage of fixed cutter bits. Several users are developing databases for fixed cutter bits in an effort to relate field performance to some of the more prominent bit design characteristics.

  19. Non-invasive prenatal diagnosis for homozygous α0-thalassemia based on paternal different SNP with allele-specific real-time fluorescence PCR: establishment and application%基于父源差异SNP位点的等位基因特异性实时荧光PCR方法建立及其在α0-地中海贫血无创产前诊断中的应用

    Institute of Scientific and Technical Information of China (English)

    刘彦慧; 吴亚敏; 石少权; 娄季武; 马秋林; 何怡; 徐婉芳; 林洋洋; 周万军

    2012-01-01

    目的 建立并评价1种纯合α0-地中海贫血排除性无创产前诊断的等位基因特异性实时荧光PCR技术(AS-qPCR).方法 用PCR微测序技术,检测SEA高风险夫妇基因缺失区内9个SNP以确定双亲差异位点;然后以AS-qPCR技术检测孕妇血浆中父源差异SNP位点,判断父方是否将正常单倍型遗传给胎儿.结果 167个家系中,160个家系找到1个或多个有双亲差异的SNP位点,检测率为98.16%;94例检测到父源性正常等位基因SNP而免于创伤性产前诊断,检出率57.67%;所有家系的绒毛或羊水基因检测结果与本方法的符合率为100%.结论 采用AS-qPCR检测孕妇血浆游离DNA中双亲差异SNP位点的方法,在孕早期即可进行纯合α0-地中海贫血的排除性无创产前诊断,可使约50%高风险孕产妇免于创伤性产前诊断.%Objective To establish and evaluate an allele-specific real-time fluorescence PCR ( AS-qPCR) assay for non-invasive exclusive prenatal diagnosis of homozygous a -thalassemia. Methods Among 9 SNPs within the SEA deletion range, the different SNPs between parents were determined with PCR micro-sequencing technology. The paternal SNP was detected in maternal plasma DNA with AS-qPCR to determine whether the paternal normal haplotype had been inherited to the fetus. Results Among 167 families, 160 were found to have one or more parents-different SNPs with the detection rates over 98. 16%. The paternal normal SNPs were detected in 94 maternal plasma DNA. In this way, they avoided invasive prenatal diagnosis with a detection rate of 57.67%. These results were 100% coincidence with the results of CVS or amniocentesis. Conclusion Using AS-qPCR to detect the parents-difference SNPs in maternal plasma DNA in the early stage of gestation, is a non-invasive exclusive prenatal diagnosis of homozygous a?thalassemia. This method can make about 50% high-risk pregnant women to avoid invasive prenatal diagnosis.

  20. An Empirically Grounded Model of the Adoption of Intellectual Technologies.

    Science.gov (United States)

    Wildemuth, Barbara M.

    1992-01-01

    Data on adoption of 43 user-developed computing applications in 3 large corporations were analyzed to develop an empirically grounded model of the adoption process for intellectual technologies. A five-stage model consisting of Resource Acquisition, Application Development, Adoption/Renewal, Routinization/Enhancement, and External Adoption was…

  1. Understanding Technology Adoption: Theory and Future Directions for Informal Learning

    Science.gov (United States)

    Straub, Evan T.

    2009-01-01

    How and why individuals adopt innovations has motivated a great deal of research. This article examines individuals' computing adoption processes through the lenses of three adoption theories: Rogers's innovation diffusion theory, the Concerns-Based Adoption Model, the Technology Acceptance Model, and the United Theory of Acceptance and Use of…

  2. Partitioning the Adoption Process to Better Predict Permanency

    Science.gov (United States)

    McDonald, Tom; Press, Alan; Billings, Peggy; Moore, Terry

    2007-01-01

    Under federal outcome standards established by the Adoption and Safe Families Act of 1997, discharges to adoption are expected to occur within 24 months of the most recent removal from home for at least 32% of cases. In the research recounted here, adoption is treated as a process composed of two discrete steps: adoptive placement, and adoption…

  3. Adoptive parenting and attachment: association of the internal working models between adoptive mothers and their late-adopted children during adolescence

    OpenAIRE

    Cecilia Serena ePace; Simona eDi Folco; Viviana eGuerriero; Alessandra eSantona; Grazia eTerrone

    2015-01-01

    Introduction: Recent literature has shown that the good outcome of adoption would mostly depend on the quality of adoptive parenting, which is strongly associated with the security of parental internal working models (IWMs) of attachment. Specifically, attachment states-of-mind of adoptive mothers classified as free and autonomous and without lack of resolution of loss or trauma could represent a good protective factor for adopted children, previously maltreated and neglected. While most rese...

  4. Adoptive parenting and attachment: association of the Internal Working Models between adoptive mothers and their late-adopted children during adolescence

    Directory of Open Access Journals (Sweden)

    Cecilia Serena ePace

    2015-09-01

    Full Text Available Introduction. Recent literature has shown that the good outcome of adoption would mostly depend on the quality of adoptive parenting, which is strongly associated with the security of parental internal working models (IWMs of attachment. Specifically, attachment states-of-mind of adoptive mothers classified as free and autonomous and without lack of resolution of loss or trauma could represent a good protective factor for adopted children previously maltreated and neglected. While most research on adoptive families has focused on pre-school and school-aged children, the aim of this study was to assess the concordance of IWMs of attachment in adoptive dyads during adolescence.Method. Our pilot-study involved 76 participants: 30 adoptive mothers (mean age=51.5±4.3, and their 46 late-adopted adolescents (mean age = 13.9 ± 1.6, who were all aged four to nine years old at time of adoption (M = 6.3 ± 1.5. Attachment representations of adopted adolescents were assessed by the Friend and Family Interview (FFI, while adoptive mothers’ state-of-mind with respect to attachment was classified by the Adult Attachment Interview (AAI. Adolescents’ verbal intelligence was controlled.Results. Late-adopted adolescents were classified as follows: 67% secure, 26% dismissing and 7% preoccupied in the FFI, while their adoptive mothers’ AAI classifications were 70% free-autonomous, 7% dismissing, and 23% unresolved. We found a significant concordance of 70% (32 dyads between the secure-insecure FFI and AAI classifications. Specifically adoptive mothers with high coherence of transcript and low unresolved loss tend to have late-adopted children with high secure attachment, even if the adolescents’ verbal intelligence made a significant contribution to this prediction.Discussion. Our results provides an empirical contribution to the literature concerning the concordance of attachment in adoptive dyads, highlighting the beneficial impact of highly coherent

  5. Polyhedral patterns

    KAUST Repository

    Jiang, Caigui

    2015-10-27

    We study the design and optimization of polyhedral patterns, which are patterns of planar polygonal faces on freeform surfaces. Working with polyhedral patterns is desirable in architectural geometry and industrial design. However, the classical tiling patterns on the plane must take on various shapes in order to faithfully and feasibly approximate curved surfaces. We define and analyze the deformations these tiles must undertake to account for curvature, and discover the symmetries that remain invariant under such deformations. We propose a novel method to regularize polyhedral patterns while maintaining these symmetries into a plethora of aesthetic and feasible patterns.

  6. Adoption: biological and social processes linked to adaptation.

    Science.gov (United States)

    Grotevant, Harold D; McDermott, Jennifer M

    2014-01-01

    Children join adoptive families through domestic adoption from the public child welfare system, infant adoption through private agencies, and international adoption. Each pathway presents distinctive developmental opportunities and challenges. Adopted children are at higher risk than the general population for problems with adaptation, especially externalizing, internalizing, and attention problems. This review moves beyond the field's emphasis on adoptee-nonadoptee differences to highlight biological and social processes that affect adaptation of adoptees across time. The experience of stress, whether prenatal, postnatal/preadoption, or during the adoption transition, can have significant impacts on the developing neuroendocrine system. These effects can contribute to problems with physical growth, brain development, and sleep, activating cascading effects on social, emotional, and cognitive development. Family processes involving contact between adoptive and birth family members, co-parenting in gay and lesbian adoptive families, and racial socialization in transracially adoptive families affect social development of adopted children into adulthood.

  7. Turing patterns and biological explanation

    DEFF Research Database (Denmark)

    Serban, Maria

    2017-01-01

    , promoting theory exploration, and acting as constitutive parts of empirically adequate explanations of naturally occurring phenomena, such as biological pattern formation. Focusing on the roles that minimal model explanations play in science motivates the adoption of a broader diachronic view of scientific...

  8. Endogenous Technology Adoption and Medical Costs.

    Science.gov (United States)

    Lamiraud, Karine; Lhuillery, Stephane

    2016-09-01

    Despite the claim that technology has been one of the most important drivers of healthcare spending growth over the past decades, technology variables are rarely introduced explicitly in cost equations. Furthermore, technology is often considered exogenous. Using 1996-2007 panel data on Swiss geographical areas, we assessed the impact of technology availability on per capita healthcare spending covered by basic health insurance whilst controlling for the endogeneity of health technology availability variables. Our results suggest that medical research, patent intensity and the density of employees working in the medical device industry are influential factors for the adoption of technology and can be used as instruments for technology availability variables in the cost equation. These results are similar to previous findings: CT and PET scanner adoption is associated with increased healthcare spending, whilst increased availability of percutaneous transluminal coronary angioplasty facilities is associated with reductions in per capita spending. However, our results suggest that the magnitude of these relationships is much greater in absolute value than that suggested by previous studies that did not control for the possible endogeneity of the availability of technologies. Copyright © 2016 John Wiley & Sons, Ltd.

  9. CRM ADOPTION IN A HIGHER EDUCATION INSTITUTION

    Directory of Open Access Journals (Sweden)

    Guy-Emmanuel Rigo

    2016-04-01

    Full Text Available More and more organisations, from private to public sectors, are pursuing higher levels of customer satisfaction, loyalty and retention. With this intent, higher education institutions (HEI have adopted CRM – Customer Relationship Management. In order to analyse some of the interesting aspects of this phenomenon n, we conducted an action research in a European Institute. The main research question we answered is “how to adopt a CRM strategy in a Higher Education Institution?” Some of the main findings of this study are (1 even though HEI’s main customer is the student, there are others stakeholders that a CRM project must consider; (2 universities can use their internal resources to implement a CRM project successfully; and (3 using Agile software methodology is an effective way to define clearer, more objective and more assertive technical requirements which result in a CRM software that meet send user’s expectations and organizational strategic goals. These findings can help other HEIs

  10. Information architecture: Profile of adopted standards

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-09-01

    The Department of Energy (DOE), like other Federal agencies, is under increasing pressure to use information technology to improve efficiency in mission accomplishment as well as delivery of services to the public. Because users and systems have become interdependent, DOE has enterprise wide needs for common application architectures, communication networks, databases, security, and management capabilities. Users need open systems that provide interoperability of products and portability of people, data, and applications that are distributed throughout heterogeneous computing environments. The level of interoperability necessary requires the adoption of DOE wide standards, protocols, and best practices. The Department has developed an information architecture and a related standards adoption and retirement process to assist users in developing strategies and plans for acquiring information technology products and services based upon open systems standards that support application software interoperability, portability, and scalability. This set of Departmental Information Architecture standards represents guidance for achieving higher degrees of interoperability within the greater DOE community, business partners, and stakeholders. While these standards are not mandatory, particular and due consideration of their applications in contractual matters and use in technology implementations Department wide are goals of the Chief Information Officer.

  11. Standardization in technology adoption: A comparison of broadcast TV cases

    OpenAIRE

    Kim, Heejung

    2012-01-01

    Based on developments in the U.S. television, this study illustrates the choice of technical standards by the government. Broadcasting in the U.S. has been largely affected by the adoption of new technology. Technology adoptions have impacted economy, society, industry, and government policies of the U.S. broadcasting. The purpose of this study is to examine standard-setting development and policies for major technology adoptions in broadcasting: color TV adoption and digital TV adoption.

  12. Child-Adoption Matching: Preferences for Gender and Race

    OpenAIRE

    Mariagiovanna Baccara; Allan Collard-Wexler; Leonardo Felli; Leeat Yariv

    2010-01-01

    This paper uses a new data set on child-adoption matching to estimate the preferences of potential adoptive parents over U.S.-born and unborn children relinquished for adoption. We identify significant preferences favoring girls and unborn children close to birth, and against African-American children put up for adoption. These attitudes vary in magnitudes across different adoptive parents - heterosexual, same-sex couples, and single women. We also consider the effects of excluding single wom...

  13. Child adoption matching: preferences for gender and race

    OpenAIRE

    Baccara, Mariagiovanna; Collard-Wexler, Allan; Felli, Leonardo; Yariv, Leeat

    2014-01-01

    This paper uses a new dataset on child-adoption matching to estimate the preferences of potential adoptive parents over US-born and unborn children relinquished for adoption. We identify significant preferences favoring girls and against African American children put up for adoption. These attitudes vary in magnitudes across different adoptive parents—heterosexual, same-sex couples, and single women. We consider the effects of excluding single women and same-sex couples from the process, and ...

  14. What drives Web 2.0 adoption in South African civil society organisations

    Directory of Open Access Journals (Sweden)

    Kiru Pillay

    2014-10-01

    Full Text Available Background: The impact and consequences of social media adoption on society are only just being realised and studied in detail; consequently, there is no universal agreement as to the reasons for the adoption of these services. Even understanding why some social media services are popular remains to some extent elusive. The practical use of Web 2.0 does not provide any answers either with, for example, a noticeable difference in the way social media was strategically used by Barack Obama and Mitch Romney in the lead-up to the 2009 American elections. However, recent studies that have focused on social media adoption within specific sectors have begun to shed some light on these emerging adoption patterns; two studies in particular are illustrative: a 2012 study on the newspaper sector and a study on social media adoption and e-government. Objectives: This study investigates why South African civil society organisations (CSOs adopt Web 2.0 services and the perceived and actual benefits of such adoption. Method: A survey questionnaire was sent to 1712 South African CSOs listed in the Prodder database to explore why certain social media services were adopted and the perceived benefits thereof. Results: Internal reasons for the adoption of social media services by South African CSOs coalesce around organisational visibility and access to information. External reasons focus on organisations needing to become more relevant and more connected to like-minded organisations and initiatives. Conclusion: The pervasiveness of Web 2.0 technologies makes it inevitable that CSOs will have to restructure themselves to remain relevant.

  15. What drives Web 2.0 adoption in South African civil society organisations

    Directory of Open Access Journals (Sweden)

    Kiru Pillay

    2014-03-01

    Full Text Available Background: The impact and consequences of social media adoption on society are only just being realised and studied in detail; consequently, there is no universal agreement as to the reasons for the adoption of these services. Even understanding why some social media services are popular remains to some extent elusive. The practical use of Web 2.0 does not provide any answers either with, for example, a noticeable difference in the way social media was strategically used by Barack Obama and Mitch Romney in the lead-up to the 2009 American elections. However, recent studies that have focused on social media adoption within specific sectors have begun to shed some light on these emerging adoption patterns; two studies in particular are illustrative: a 2012 study on the newspaper sector and a study on social media adoption and e-government.Objectives: This study investigates why South African civil society organisations (CSOs adopt Web 2.0 services and the perceived and actual benefits of such adoption.Method: A survey questionnaire was sent to 1712 South African CSOs listed in the Prodder database to explore why certain social media services were adopted and the perceived benefits thereof.Results: Internal reasons for the adoption of social media services by South African CSOs coalesce around organisational visibility and access to information. External reasons focus on organisations needing to become more relevant and more connected to like-minded organisations and initiatives.Conclusion: The pervasiveness of Web 2.0 technologies makes it inevitable that CSOs will have to restructure themselves to remain relevant.

  16. Specialization Patterns

    DEFF Research Database (Denmark)

    Schultz, Ulrik Pagh; Lawall, Julia Laetitia; Consel, Charles

    2000-01-01

    Design patterns offer many advantages for software development, but can introduce inefficiency into the final program. Program specialization can eliminate such overheads, but is most effective when targeted by the user to specific bottlenecks. Consequently, we propose that these concepts...... are complementary. Program specialization can optimize programs written using design patterns, and design patterns provide information about the program structure that can guide specialization. Concretely, we propose specialization patterns, which describe how to apply program specialization to optimize uses...... of design patterns. In this paper, we analyze the specialization opportunities provided by specific uses of design patterns. Based on the analysis of each design pattern, we define the associated specialization pattern. These specialization opportunities can be declared using the specialization classes...

  17. Allele-specific regulation of MTTP expression influences the risk of ischemic heart disease

    DEFF Research Database (Denmark)

    Aminoff, Anna; Ledmyr, Helena; Thulin, Petra;

    2010-01-01

    Promoter polymorphisms in microsomal triglyceride transfer protein (MTTP) have been associated with decreased plasma lipids but an increased risk for ischemic heart disease (IHD), indicating that MTTP influences the susceptibility for IHD independent of plasma lipids. The objective of this study...

  18. Rapid identification of capybara (Hydrochaeris hydrochaeris through allele-specific PCR

    Directory of Open Access Journals (Sweden)

    Flávio Henrique-Silva

    2005-07-01

    Full Text Available The capybara is the largest rodent in the world and is widely distributed throughout Central and South America.  It is an animal of economic interest due to the pleasant flavor of its meat and higher protein content in comparison  to beef and pork meat.  The hide, hair and fat also have economic advantages. Thus,  as an animal with such high economic potential, it is the target of hunters, even though  hunting capybara is prohibited by law in Brazil.   Due to their  similarities,  capybara meat  is easily confused with  pork  meat.   This  occurs  upon  the apprehension of the  meat  from hunters, as well as in some restaurants that serve capybara meat that was slaughtered clandestinely. In both cases, when the meat is confiscated, those responsible for the crimes claim it is pork meat,  hindering  the enforcement of the law. A practical  course was ministered  to undergraduate biology students enrolled in the elective course Introduction to Genetic  Engineering  at Federal  University  of Sao Carlos (UFSCar, Sao Paulo  State, Brazil.  The  objective  of the  course was to establish  and  apply  a Polymerase  Chain  Reaction  (PCR assay to identify capybara meat and discriminate it in relation  to other types of meat,  including pork. Primers  were designed based  on 12S rRNA,  transthyretin and  growth  hormone  receptor  genes.  The primers generated  capybara specific fragments  of approximately 220, 290 and 330 bp for transthyretin,12S rRNA  and  growth  hormone  receptor,  respectively.   The  duplexes  developed  in the  present work can be used effectively to discriminate capybara meat  from other  animals,  contributing to combating predatory capybara hunting. The results were extensively discussed and the students have contributed to written a paper  to be submitted to a publication.

  19. Rapid identification of capybara (Hydrochaeris hydrochaeris) through allele-specific PCR

    OpenAIRE

    2005-01-01

    The capybara is the largest rodent in the world and is widely distributed throughout Central and South America.  It is an animal of economic interest due to the pleasant flavor of its meat and higher protein content in comparison  to beef and pork meat.  The hide, hair and fat also have economic advantages. Thus,  as an animal with such high economic potential, it is the target of hunters, even though  hunting capybara is prohibited by law in Brazil.   Due to their  similarities,  capybara me...

  20. Sequenza: allele-specific copy number and mutation profiles from tumor sequencing data

    DEFF Research Database (Denmark)

    Favero, Francesco; Joshi, Tejal; Marquard, Andrea Marion;

    2015-01-01

    Background : Exome or whole genome deep sequencing of tumor DNA along with paired normal DNA can potentially provide a detailed picture of the somatic mutations that characterize the tumor. However, analysis of such sequence data can be complicated by the presence of normal cells in the tumor spe...

  1. DQB1*06:02 allele-specific expression varies by allelic dosage, not narcolepsy status

    DEFF Research Database (Denmark)

    Weiner Lachmi, Karin; Lin, Ling; Kornum, Birgitte Rahbek;

    2012-01-01

    The association of narcolepsy-cataplexy, a sleep disorder caused by the loss of hypocretin/orexin neurons in the hypothalamus, with DQA1*01:02-DQB1*06:02 is one of the tightest known single-allele human leukocyte antigen (HLA) associations. In this study, we explored genome-wide expression...... in peripheral white blood cells of 50 narcolepsy versus 47 controls (half of whom were DQB1*06:02 positive) and observed the largest differences between the groups in the signal from HLA probes. Further studies of HLA-DQ expression (mRNA and protein in a subset) in 125 controls and 147 narcolepsy cases did...... indicate that allelic dosage is transmitted into changes in heterodimer availability, a phenomenon that may explain the increased risk for narcolepsy in DQB1*06:02 homozygotes versus heterozygotes....

  2. Allele-specific up-regulation of FGFR2 increases susceptibility to breast cancer.

    Directory of Open Access Journals (Sweden)

    Kerstin B Meyer

    2008-05-01

    Full Text Available The recent whole-genome scan for breast cancer has revealed the FGFR2 (fibroblast growth factor receptor 2 gene as a locus associated with a small, but highly significant, increase in the risk of developing breast cancer. Using fine-scale genetic mapping of the region, it has been possible to narrow the causative locus to a haplotype of eight strongly linked single nucleotide polymorphisms (SNPs spanning a region of 7.5 kilobases (kb in the second intron of the FGFR2 gene. Here we describe a functional analysis to define the causative SNP, and we propose a model for a disease mechanism. Using gene expression microarray data, we observed a trend of increased FGFR2 expression in the rare homozygotes. This trend was confirmed using real-time (RT PCR, with the difference between the rare and the common homozygotes yielding a Wilcox p-value of 0.028. To elucidate which SNPs might be responsible for this difference, we examined protein-DNA interactions for the eight most strongly disease-associated SNPs in different breast cell lines. We identify two cis-regulatory SNPs that alter binding affinity for transcription factors Oct-1/Runx2 and C/EBPbeta, and we demonstrate that both sites are occupied in vivo. In transient transfection experiments, the two SNPs can synergize giving rise to increased FGFR2 expression. We propose a model in which the Oct-1/Runx2 and C/EBPbeta binding sites in the disease-associated allele are able to lead to an increase in FGFR2 gene expression, thereby increasing the propensity for tumour formation.

  3. Allele-specific down-regulation of RPTOR expression induced by retinoids contributes to climate adaptations.

    Directory of Open Access Journals (Sweden)

    Chang Sun

    2010-10-01

    Full Text Available The mechanistic target of rapamycin (MTOR pathway regulates cell growth, energy homeostasis, apoptosis, and immune response. The regulatory associated protein of MTOR encoded by the RPTOR gene is a key component of this pathway. A previous survey of candidate genes found that RPTOR contains multiple SNPs with strong correlations between allele frequencies and climate variables, consistent with the action of selective pressures that vary across environments. Using data from a recent genome scan for selection signals, we honed in on a SNP (rs11868112 26 kb upstream to the transcription start site of RPTOR that exhibits the strongest association with temperature variables. Transcription factor motif scanning and mining of recently mapped transcription factor binding sites identified a binding site for POU class 2 homeobox 1 (POU2F1 spanning the SNP and an adjacent retinoid acid receptor (RAR binding site. Using expression quantification, chromatin immunoprecipitation (ChIP, and reporter gene assays, we demonstrate that POU2F1 and RARA do bind upstream of the RPTOR gene to regulate its expression in response to retinoids; this regulation is affected by the allele status at rs11868112 with the derived allele resulting in lower expression levels. We propose a model in which the derived allele influences thermogenesis or immune response by altering MTOR pathway activity and thereby increasing fitness in colder climates. Our results show that signatures of genetic adaptations can identify variants with functional effects, consistent with the idea that selection signals may be used for SNP annotation.

  4. A four-element based transposon system for allele specific tagging in plants – Theoretical considerations

    Indian Academy of Sciences (India)

    Sanjay Phogat; Pradeep Kumar Burma; Deepak Pental

    2000-03-01

    The two-element transposon constructs, utilizing either Ac/Ds or Spm/dSpm, allow random tagging of genes in heterologous model species, but are inadequate for directed tagging of specific alleles of agronomic importance. We propose the use of Ac/Ds in conjunction with Spm/dSpm to develop a four-element system for directed tagging of crop-specific alleles. The four-element based construct would include both Ds and dSpm along with relevant marker genes and would function in two steps. In the first step dSpm(Ds) stocks (a minimum of two) would be crossed to a line containing transposases of Spm and unlinked integrations would be selected from segregating population by the use of a negative selection marker to develop stocks representing integration of dSpm(Ds) at a large number of locations in the genome. Selections would be made for a line in which dSpm(Ds) shows partial or complete linkage to the allele of interest. In the second step selected line would be crossed to a line containing Ac transposase to induce transpositions of Ds element to linked sites thereby exploiting the natural tendency of Ds element to jump to linked sites. Unlinked jumps of dSpm(Ds) and linked jumps of Ds could be monitored by appropriate marker genes. The proposed model would allow tagging of allele of interest in chromosome addition lines and also help in the efficient use of genic male sterility systems for hybrid seed production by tightly marking the fertility restorer gene with a negative selection marker.

  5. Using Allele-Specific PCR with Molecular Beams as a Means for Genotyping the Diallelic Indels

    Energy Technology Data Exchange (ETDEWEB)

    Doktycz, M.J.; Weber, J.L. (Marshfield Medical Research Foundation)

    2000-06-01

    The first Specific Aim for this grant was to identify and characterize an average of 500 human insertion/deletion polymorphisms per grant year (1500 total). This task was carried out entirely at MMRF. They substantially exceeded this goal by confirming about 2,300 diallelic indels. Complete characterization information for these polymorphisms is available from the Marshfield web site. A manuscript describing results for the first 2,000 diallelic indels was published earlier this year in the American Journal of Human Genetics. The Second Specific Aim of the grant was to investigate and develop improved methods for analysis of diallelic polymorphisms using miniaturized DNA arrays. The initial genotyping technology efforts focused on various hybridization and extension protocols with oligo arrays on flow-through channel glass. Channel glass is a porous material that permits reagents to be passed through the arrays. They devoted roughly 19 months at the beginning of the grant in pursuit of this methodology, but for various technological reasons, progress was limited.

  6. Lack of allele-specific efficacy of a bivalent AMA1 malaria vaccine

    Directory of Open Access Journals (Sweden)

    Ellis Ruth D

    2010-06-01

    Full Text Available Abstract Background Extensive genetic diversity in vaccine antigens may contribute to the lack of efficacy of blood stage malaria vaccines. Apical membrane antigen-1 (AMA1 is a leading blood stage malaria vaccine candidate with extreme diversity, potentially limiting its efficacy against infection and disease caused by Plasmodium falciparum parasites with diverse forms of AMA1. Methods Three hundred Malian children participated in a Phase 2 clinical trial of a bivalent malaria vaccine that found no protective efficacy. The vaccine consists of recombinant AMA1 based on the 3D7 and FVO strains of P. falciparum adjuvanted with aluminum hydroxide (AMA1-C1. The gene encoding AMA1 was sequenced from P. falciparum infections experienced before and after immunization with the study vaccine or a control vaccine. Sequences of ama1 from infections in the malaria vaccine and control groups were compared with regard to similarity to the vaccine antigens using several measures of genetic diversity. Time to infection with parasites carrying AMA1 haplotypes similar to the vaccine strains with respect to immunologically important polymorphisms and the risk of infection with vaccine strain haplotypes were compared. Results Based on 62 polymorphic AMA1 residues, 186 unique ama1 haplotypes were identified among 315 ama1 sequences that were included in the analysis. Eight infections had ama1 sequences identical to 3D7 while none were identical to FVO. Several measures of genetic diversity showed that ama1 sequences in the malaria vaccine and control groups were comparable both at baseline and during follow up period. Pre- and post-immunization ama1 sequences in both groups all had a similar degree of genetic distance from FVO and 3D7 ama1. No differences were found in the time of first clinical episode or risk of infection with an AMA1 haplotype similar to 3D7 or FVO with respect to a limited set of immunologically important polymorphisms found in the cluster 1 loop of domain I of AMA1. Conclusion This Phase 2 trial of a bivalent AMA1 malaria vaccine found no evidence of vaccine selection or strain-specific efficacy, suggesting that the extreme genetic diversity of AMA1 did not account for failure of the vaccine to provide protection.

  7. Allele-specific expression of the low density lipoprotein receptor gene

    Energy Technology Data Exchange (ETDEWEB)

    Minnich, A.; Lussier-Cacan, S.; Roy, M. [Clincial Research Institute of Montreal, Quebec (Canada)

    1994-09-01

    Approximately 60% of familial hypercholesterolemia (FH) in French Canadians is due to a > 10 kb deletion of the promoter region of the gene encoding the low density lipoprotein (LDL) receptor (LDL-R), allowing determination of the influence of a single LDL-R allele on phenotypic expression of FH. Normal allele haplotypes of approximately 250 heterozygotes were determined with 7 RFLPs. In vitro maximal LDL-R activity of blood lymphocytes from a subset of approximately 150 heterozygotes, measured by immunocytofluorometry, was significantly higher (20 to 30%) in subjects with LDL-R normal allele haplotype G (n=11), and O (n=7) compared to the most frequent haplotype F (n=43), while no differences were observed among F, E (n=11), and the 2 other most prevalent haplotypes (n=43). LDL-R mRNA in these lymphocytes was significantly elevated 2.3-, 1.7-, and 1.8- fold, in G, O, and E, respectively, compared to F, while no significant differences were apparent between F and the other two most frequent haplotyes. Large interindividual variability in lymphocyte LDL-R mRNA levels and activity was observed even among subjects with the same LDL-R normal allele haplotype. However, maximally induced lymphocyte LDL-R mRNA levels correlated poorly with levels measured in freshly isolated cells (n=14). Relative to haplotype F (n=47 women (W), 39 men (M)), mean plasma LDL cholesterol levels adjusted for age and apolipoprotein E genotype were 5-10% lower in men and women with haplotypes G (n=16 W, 12 M) and O (n=8 W, 6 M), and 20% lower in 7 W with haplotype E. These results suggest that (1) normal LDL-R allele haplotype G and O may contain sequence variations which confer relatively high gene expression and (2) environmental and genetic influences other than the LDL-R gene contribute substantially to variability in LDL-R expression and plasma LDL cholesterol levels in French Canadian FH heterozygotes.

  8. The crystal structures and powder diffraction patterns of the uranium tellurides

    Energy Technology Data Exchange (ETDEWEB)

    Snyder, R.L. (State Univ. of New York, Alfred, NY (USA). Inst. of Ceramic Superconductivity); Nichols, M.C.; Boehme, D.R. (Sandia National Labs., Livermore, CA (USA))

    1990-10-03

    A critical review of all of the reported structures and powder diffraction patterns in the uranium telluride system has been undertaken. Structures that are correct: Cubic -- UTe: no experimental pattern exists. Retain calculated 15--865. Cubic --U{sub 3}Te{sub 4}: retain the poor quality 12--610 but adopt the pattern calculated here. Cubic U{sub 2}Te{sub 3}: no experimental pattern exists. Adopt pattern calculated here. Orthorhombic UTe{sub 2}: Adopt the new pattern of Boehme et al. Monoclinic {alpha}UTe{sub 3} Adopt the new pattern of Boehme et al. Monoclinic {alpha}UTe{sub 3} Adopt the new pattern of Boehme et al. Orthorhombic {beta}UTe{sub 3}: Adopt pattern calculated here. Orthorhombic UTe{sub 5}: Adopt the new pattern of Boehme et al. Structures in need of refinement: Orthorhombic U{sub 2}Te{sub 3}:Adopt pattern calculated here over 34--807. Hexagonal U{sub 7}Te{sub 12}: Adopt pattern calculated here but retain 24--1368. Orthorhombic UTe{sub 1.78}: Adopt pattern calculated here and retain our modified 21--1404 reported for U{sub 4}Te{sub 7}. Orthorhombic UTe{sub 2.5}: Adopt pattern calculated here. Orthorhombic UTe{sub 3.4}: Accept recent pattern of Boehme et al. Phases for which no structures or reliable patterns exist: Orthorhombic U{sub 3}Te{sub 4}: no published pattern. Tetragonal U{sub 3}Te{sub 5}: three patterns 21--1407, 34--766 and 34--896 exit but all are of very poor quality. Phases which probably do not exist: Tetragonal UTe{sub 1.78}, Tetragonal UTe{sub 2}, Cubic UTe{sub 2} U{sub 3}Te{sub 7}(21--1402), U{sub 3}Te{sub 8}(21--1406).

  9. Wide-Scale Adoption of Photovoltaic Energy

    DEFF Research Database (Denmark)

    Yang, Yongheng; Enjeti, Prasad; Blaabjerg, Frede

    2015-01-01

    Current grid standards largely require that low-power (e.g., several kilowatts) single-phase photovoltaic (PV) systems operate at unity power factor (PF) with maximum power point tracking (MPPT), and disconnect from the grid under grid faults by means of islanding detection. However, in the case...... of wide-scale penetration of single-phase PV systems in the distributed grid, disconnection under grid faults can contribute to 1) voltage flickers, 2) power outages, and 3) system instability. This article explores grid code modifications for a wide-scale adoption of PV systems in the distribution grid....... In addition, based on the fact that Italy and Japan have recently undertaken a major review of standards for PV power conversion systems connected to low-voltage networks, the importance of low voltage ride-through (LVRT) for single-phase PV power systems under grid faults is considered, along with three...

  10. BUSINESS INTELLIGENCE ADOPTION IN LARGE ROMANIAN COMPANIES

    Directory of Open Access Journals (Sweden)

    Flavia CAIA

    2014-11-01

    Full Text Available The economic conditions and market competition create pressures on companies to adopt new technologies that can provide more efficient information and can support decision-making better. The purpose of the research is to investigate the decision support information systems in order to apprise and enhance the capacity of the entities to apply the new knowledge that BI produces for organizational success and competitiveness. The importance of the conducted research consists in identifying solutions to improve reporting and stimulate the entities to start using business intelligence (BI technologies, which facilitate obtaining new information, in order to ensure flexibility, resilience and provide answers to questions that go beyond what the pre-defined reports can do to support decision-making. The estimated result is a technical and operational overview of the large companies in Romania, drawing future directions for an improved competitive behaviour and strategic awareness, and identifying the significant factors for optimizing the decision-making process.

  11. Green innovation adoption in automotive supply chain

    DEFF Research Database (Denmark)

    Zailani, Suhaiza; Govindan, Kannan; Iranmanesh, Mohammad;

    2015-01-01

    using the partial least squares technique. Environmental regulations, market demand, and firm internal initiatives have a positive effect on green innovation initiatives (GII), while GIIs have a positive effect on the three categories of sustainable performance (i.e., environmental, social, and economic......Green innovation has currently been receiving a great deal of international attention because of the growing concern of consumers, governments, and the community as a whole with regard to the degradation of natural resources and environmental pollution. The automotive sector is one of the leading...... generators of industrial waste that affect the quality of the natural environment. This study aims to investigate the determinants of green innovation adoption and its effect on firm performance. Data were gathered by surveying 153 firms in the Malaysian automotive supply chain industry. Data were analyzed...

  12. The Adoption of Innovations in Library Organizations

    Directory of Open Access Journals (Sweden)

    Su-may Sheih Chen

    2001-12-01

    Full Text Available To enhance the quality of library and information service, libraries have borrowed from business theory and practice in planning and evaluating library services. However, an area of business theory that has received relatively little attention in library science is organizational innovation. Reports in the business literature provide evidence that innovation is essential for both development and survival. In increasingly unstable times of rapidly changing information technology, libraries must use the literature of innovation as well as that of planning and evaluation to shape better libraries. This paper explores key issues related to innovation in libraries including the nature, the barriers, and the ways to adopt and implement innovative strategies successfully. [Article content in Chinese

  13. Home Energy Displays. Consumer Adoption and Response

    Energy Technology Data Exchange (ETDEWEB)

    LaMarche, Janelle [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Cheney, K. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Akers, C. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Roth, K. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States); Sachs, O. [Fraunhofer Center for Sustainable Energy Systems, Cambridge, MA (United States)

    2012-12-01

    The focus of this project was to investigate the factors influencing consumer adoption of Home Energy Displays (HEDs) and to evaluate electricity consumption in households with basic HEDs versus enhanced feedback methods - web portals or alerts. The team hypothesized that providing flexible and relatable information to users, in addition to a basic HED, would make feedback more effective and achieve persistent energy savings. In Phase I, Fraunhofer conducted three user research studies and found preferences for aesthetically pleasing, easy to understand feedback that is accessible through multiple media and offered free of charge. The deployment of HEDs in 150 households planned for Phase II encountered major recruitment and HED field deployment problems. In light of these challenges, the team is pursuing a modified study investigating the energy savings of a web portal versus alert-based energy feedback instead of a physical HED.

  14. Pattern matching

    OpenAIRE

    Hak, Tony; Dul, Jan

    2009-01-01

    textabstractPattern matching is comparing two patterns in order to determine whether they match (i.e., that they are the same) or do not match (i.e., that they differ). Pattern matching is the core procedure of theory-testing with cases. Testing consists of matching an “observed pattern” (a pattern of measured values) with an “expected pattern” (a hypothesis), and deciding whether these patterns match (resulting in a confirmation of the hypothesis) or do not match (resulting in a disconfirmat...

  15. Adoption of Mobile Banking Applications in Lebanon

    Directory of Open Access Journals (Sweden)

    Marc F Audi

    2016-04-01

    Full Text Available This research studied the impact of mobile banking adaptation in Lebanon. A brief overview of the Lebanese banking sector has shown how it is very dynamic and large. The overview also shows that the Lebanese banking sector is very competitive and changing with the development of new technologies and the availability of new services. To explain mobile banking applications in Lebanon two models were studied in the literature. The first is the innovation diffusion theory which has 5 different constructs: relative advantage, compatibility, communicability, trailability and complexity. The innovation diffusion theory talks about the path innovations take to reach final adopters. The technology acceptance model on the other hand relates the effects of it main components, perceived ease of use and perceived usefulness, on consumer attitude and intention to adopt. Our model is based on both theories and has 8 constructs. It shows the effect of the 5 constructs of the innovation diffusion theory merging perceived usefulness to relative advantage and replacing complexity with perceived ease of use. We have developed 8 hypotheses based on the construct of the model and to study the user-none user effect. A personal administered survey done using convenient sampling managed to gather 315 respondents from 5 different locations in Beirut after administering pilot testing. All variables were tested for validity and reliability using different tests. A biverate linear regression analysis was used to test the hypotheses and establish the relations between the different variables. We also did an independent t-test to analyze the user none-user effects. It is concluded that this paper with a discussion of the tests and recommending the best way to market mobile banking applications and designing a good version of mobile banking that looks appealing to consumers.

  16. Adoption of information technology by resident physicians.

    Science.gov (United States)

    Parekh, Selene G; Nazarian, David G; Lim, Charles K

    2004-04-01

    The Internet represents a technological revolution that is transforming our society. In the healthcare industry, physicians have been typified as slow adopters of information technology. However, young physicians, having been raised in a computer-prevalent society, may be more likely to embrace technology. We attempt to characterize the use and acceptance of the Internet and information technology among resident physicians in a large academic medical center and to assess concerns regarding privacy, security, and credibility of information on the Internet. A 41-question survey was distributed to 150 pediatric, medical, and surgical residents at an urban, academic medical center. One hundred thirty-five residents completed the survey (response rate of 90%). Responses were evaluated and statistical analysis was done. The majority of resident physicians in our survey have adopted the tools of information technology. Ninety-eight percent used the Internet and 96% use e-mail. Two-thirds of the respondents used the Internet for healthcare-related purposes and a similar percentage thought that the Internet has affected their practice of medicine positively. The majority of residents thought that Internet healthcare services such as electronic medical records, peer-support websites, and remote patient monitoring would be beneficial for the healthcare industry. However, they are concerned about the credibility, privacy, and security of health and medical information online. The majority of resident physicians in our institution use Internet and information technology in their practice of medicine. Most think that the Internet will continue to have a beneficial role in the healthcare industry.

  17. Pig design patterns

    CERN Document Server

    Pasupuleti, Pradeep

    2014-01-01

    Pig makes Hadoop programming simple, intuitive, and fun to work with. It removes the complexity from Map Reduce programming by giving the programmer immense power through its flexibility. What used to be extremely lengthy and intricate code written in other high level languages can now be written in almost one tenth of the size using its easy to understand constructs. Pig has proven to be the easiest way to learn how to program Hadoop clusters, as evidenced by its widespread adoption. This comprehensive guide enables readers to readily use design patterns to simplify the creation of complex da

  18. Toward a Process View in Adoption of Interorganizational Information Systems

    DEFF Research Database (Denmark)

    Brandt, Charlotte J.

    2014-01-01

    review found four different streams of research: 1) studies explaining why organizations adopt or reject IOIS by investigating factors determining adoption of or barriers to IOIS adoption; 2) studies explaining how to create the desired organizational effects from IOIS; 3) studies explaining how IOIS...... are affecting the buyer-supplier relationship they are introduced into; and 4) studies categorizing the structure of IOIS adopters in different configurations. Seemingly only limited effort has been made to study the actual adoption process. This apparent lack of studies of the adoption process calls...

  19. CONFLICT OF LAWS & FAMILY LAW-Adoption(1)

    Institute of Scientific and Technical Information of China (English)

    YANG KE

    2008-01-01

    Adoption involves the process by which the existing par ents cease to be the legal parents of the child and the adopters are substituted for the parents as if the child was born to them. There are so many reasons for adoption,such as inability to conceive,parents not wanting a child,and inability to provide circumstances essential for the child's healthy development.However,as the number of children available for adoption in New Zealand decreases, an increas ing number of couples look to overseas countries to adopt. Therefore,the transnational adoptions are becoming common.

  20. Gender and racial biases: Evidence from child adoption

    OpenAIRE

    Baccara, Mariagiovanna; Collard-Wexler, Allan; Felli, Leonardo; Yariv, Leeat

    2010-01-01

    This paper uses a new data set on domestic child adoption to document the preferences of potential adoptive parents over born and unborn babies relinquished for adoption by their birth mothers. We show that adoptive parents exhibit significant biases in favor of girls and against African-American babies. A non-African-American baby relinquished for adoption attracts the interest of potential adoptive parents with probability 11.5% if it is a girl and 7.9% if it is a boy. As for race, a non-Af...

  1. Single Parent Adoptions: An Issue of Difficulty and Import for Adoption Agencies.

    Science.gov (United States)

    Rosenberg, Jeffrey

    Little theoretical and empirical knowledge is available in the social work field to guide practitioners in the area of single parent adoption. In the context of demographics, the single parent family is now conventional. However there is not yet conclusive research on the success or failure of single parent households. Research has suggested that…

  2. PET imaging of adoptive progenitor cell therapies.

    Energy Technology Data Exchange (ETDEWEB)

    Gelovani, Juri G.

    2008-05-13

    Objectives. The overall objective of this application is to develop novel technologies for non-invasive imaging of adoptive stem cell-based therapies with positron emission tomography (PET) that would be applicable to human patients. To achieve this objective, stem cells will be genetically labeled with a PET-reporter gene and repetitively imaged to assess their distribution, migration, differentiation, and persistence using a radiolabeled reporter probe. This new imaging technology will be tested in adoptive progenitor cell-based therapy models in animals, including: delivery pro-apoptotic genes to tumors, and T-cell reconstitution for immunostimulatory therapy during allogeneic bone marrow progenitor cell transplantation. Technical and Scientific Merits. Non-invasive whole body imaging would significantly aid in the development and clinical implementation of various adoptive progenitor cell-based therapies by providing the means for non-invasive monitoring of the fate of injected progenitor cells over a long period of observation. The proposed imaging approaches could help to address several questions related to stem cell migration and homing, their long-term viability, and their subsequent differentiation. The ability to image these processes non-invasively in 3D and repetitively over a long period of time is very important and will help the development and clinical application of various strategies to control and direct stem cell migration and differentiation. Approach to accomplish the work. Stem cells will be genetically with a reporter gene which will allow for repetitive non-invasive “tracking” of the migration and localization of genetically labeled stem cells and their progeny. This is a radically new approach that is being developed for future human applications and should allow for a long term (many years) repetitive imaging of the fate of tissues that develop from the transplanted stem cells. Why the approach is appropriate. The novel approach to

  3. Adoption of alternative transport technologies in the construction industry

    NARCIS (Netherlands)

    Habets, Thijs; Voordijk, Hans; Sijde, van der Peter

    2006-01-01

    This research examines how the construction industry adopts alternative transport technologies. This paper presents the general characteristics of the adopter and what his perceptions are towards innovative transport technologies. The study focused on four rates of innovation, related tot alternativ

  4. Investors’ Adoption of Internet Stock Trading: A Study

    Directory of Open Access Journals (Sweden)

    Arwinder Singh

    2010-04-01

    Full Text Available The purpose of this paper wa s to examine whether investors who adopted Internet stock trading perceived differently from those of non - adopters. The primary data based on 299 investors (149 adopters and 150 non - adopters were analyzed using advanced multivariate techniques like factor analysis and discriminant analysis besides percentages, means, and Z - test. Results indicated that a ttitude dimensions and demographic variabl es contributed significantly in classifying investors as adopters or non - adopters in Internet trading. As regards attitude dimensions, ‘ variety of financial products and safety’ contributed significantly in discriminating between adopters and non - adopters of Internet trading followed by the factor such as ‘ convenience and transparency’ . As far as the demographics were concerned, the mature/older, experienced, and businessmen investors were less likely to use Internet stock trading as compared to young, inex perienced, and non - businessmen investors

  5. Assessing multifunctional innovation adoption via an integrative model

    NARCIS (Netherlands)

    Sääksjärvi, M.; Samiee, S.

    2010-01-01

    This study proposes and tests an integrative model that incorporates the mental resources framework (MOA: motivation, opportunity, and ability) alongside traditional innovation adoption predictors for assessing the adoption of dual-functionality innovations (DFI), a special case of multifunctional i

  6. Pattern recognition

    CERN Document Server

    Theodoridis, Sergios

    2003-01-01

    Pattern recognition is a scientific discipline that is becoming increasingly important in the age of automation and information handling and retrieval. Patter Recognition, 2e covers the entire spectrum of pattern recognition applications, from image analysis to speech recognition and communications. This book presents cutting-edge material on neural networks, - a set of linked microprocessors that can form associations and uses pattern recognition to ""learn"" -and enhances student motivation by approaching pattern recognition from the designer's point of view. A direct result of more than 10

  7. LEARNING STYLES ADOPTED BY MEDICAL STUDENTS

    Directory of Open Access Journals (Sweden)

    Chinmay Shah

    2011-12-01

    Full Text Available Learning results in gain of knowledge, skills and attitudes. Some like to learn by seeing, some by hearing and some by demonstration. Learning style influences the retention of information and depth of comprehension. Understanding their preferred learning styles as visual, auditory, read-write or kinesthetic learners will help improve the teaching methods adopted. Role of the educator necessitates making the most of each teaching opportunity by understanding the characteristics of the learning audience and incorporating demonstrated principles of adult educational design, with a focus on collaborative learning and variety in presentation techniques. The goal is to provide student oriented education, producing efficient doctors. A cross-sectional study among 92 medical students of the Govt. Medical College, Bhavnagar, conducted in 2009. VARK questionnaire was used to access their learning preference. Preference for different learning styles were, visual (V 1.08%, auditory (A 20.65%, reading/writing (R 2.17% and kinesthetic (K 17.39%. 41.30% of the total 92 students preferred a single mode of information presentation. Of the 92 students who preferred multiple modes of information presentation, some preferred two modes (bimodal, 31.52%, some preferred three modes (tri-modal, 27.13%, and only one student preferred quadri-modal.

  8. Home Energy Displays: Consumer Adoption and Response

    Energy Technology Data Exchange (ETDEWEB)

    LaMarche, J.; Cheney, K.; Akers, C.; Roth, K.; Sachs, O.

    2012-12-01

    The focus of this project was to investigate the factors influencing consumer adoption of Home Energy Displays (HEDs) and to evaluate electricity consumption in households with basic HEDs versus enhanced feedback methods - web portals or alerts. We hypothesized that providing flexible and relatable information to users, in addition to a basic HED, would make feedback more effective and achieve persistent energy savings. In Phase I, we conducted three user research studies and found preferences for aesthetically pleasing, easy to understand feedback that is accessible through multiple media and offered free of charge. The deployment of HEDs in 150 households planned for Phase II encountered major recruitment and HED field deployment problems. First, after extensive outreach campaigns to apartment complexes with 760 units, only 8% of building's tenants elected to receive a free HED in their homes as part of the field study. Second, the HED used, a leading market model, had a spectrum of problems, including gateway miscommunications, failure to post to a data-hosting third party, and display malfunctions. In light of these challenges, we are pursuing a modified study investigating the energy savings of a web portal versus alert-based energy feedback instead of a physical HED.

  9. Should health care managers adopt Theory Z?

    Science.gov (United States)

    Safranski, S R; Kwon, I W; Walker, W R; Unger, M

    1986-04-01

    Health care administrators should carefully consider the situations in which they apply management methods used in industry, since such methods may not be effective in motivating certain groups of hospital employees. Physicians, for example, may display little loyalty to the health care organization, even though as a group they exert significant influence on policies, standards, and administration. As a result, management styles such as Theory Z that focus on holistic concern, individual decision-making responsibility, and long-term employment guarantees may fail to interest them. Nurses also may be reluctant to commit themselves to an organization because of the high rate of turnover in their profession in recent years. Support staff, however, probably would be receptive to management techniques that offer security through long-term employment guarantees. Other factors necessary for the effective use of Theory Z industrial management techniques are a clear hierarchy with well-defined reporting relationships, moderately specialized career paths, and trust among employees that the organization's concern for their welfare is genuine. The key consideration, however, in applying any theory is that only those aspects which best serve the organization's needs should be adopted.

  10. Adopting Intercultural Communication Issue in Teaching English

    Directory of Open Access Journals (Sweden)

    Hussain Ahmed Liton

    2016-05-01

    Full Text Available Cultural assimilation and intercultural awareness has been an important hub of modern language and communication studies, underlining a shift that reflects a greater significance of the inseparability of language and culture, and the need to prepare students for effective intercultural communication to thrive in a global work environment. A thriving global workplace requires effective communication skill across cultures in this era of globalization and mass migration. Under the tutelage of such backdrop, this article addresses the terrain of adopting intercultural awareness in EFL classroom teaching/learning and aims at linking culture with language in pursuit of excellence in borderless effective communication. The study, in other words, investigated the linguistic aspects that could be affected by certain cultural dimensions (e.g., beliefs, traditions, taboo words, habits, and norms, religion, social factors, etc. in intercultural communication. This paper uses a questionnaire device to receive some university teachers’ self-reported feedback. This article maintains qualitative and quantitative research methodology. The analytical research result shows that in teaching English, it is necessary to incorporate and develop aspects of cross-cultural awareness as a part of course curriculum to immerse students in effective intercultural communicative competence (ICC.

  11. Innovation Adoption: A Review of Theories and Constructs

    OpenAIRE

    Wisdom, Jennifer P.; Chor, Ka Ho Brian; Hoagwood, Kimberly E; Horwitz, Sarah M.

    2014-01-01

    Many theoretical frameworks seek to describe the dynamic process of the implementation of innovations. Little is known, however, about factors related to decisions to adopt innovations and how the likelihood of adoption of innovations can be increased. Using a narrative synthesis approach, this paper compared constructs theorized to be related to adoption of innovations proposed in existing theoretical frameworks in order to identify characteristics likely to increase adoption of innovations....

  12. Can gay and lesbian parents promote healthy development in high-risk children adopted from foster care?

    Science.gov (United States)

    Lavner, Justin A; Waterman, Jill; Peplau, Letitia Anne

    2012-10-01

    Adoption is known to promote cognitive and emotional development in children from foster care, but policy debates remain regarding whether children adopted by gay and lesbian parents can achieve these positive outcomes. This study compared the cognitive development and behavior problems at 2, 12, and 24 months postplacement of 82 high-risk children adopted from foster care in heterosexual and gay or lesbian households. On average, children in both household types showed significant gains in cognitive development and maintained similar levels of behavior problems over time, despite gay and lesbian parents raising children with higher levels of biological and environmental risks prior to adoptive placement. Results demonstrated that high-risk children show similar patterns of development over time in heterosexual and gay and lesbian adoptive households.

  13. Drivers of Adoption and Implementation of Internet-Based Marketing Channels

    DEFF Research Database (Denmark)

    Nielsen, Jørn Flohr; Mols, Niels Peter; Høst, Viggo

    2007-01-01

    This chapter analyses factors influencing manufacturers= adoption and implementation of Internet-based marketing channels, using models based on marketing channel and organisational innovation theory. Survey data from 1163 Danish, Finnish, and Swedish manufacturers form the empirical basis...... for testing the models using LISREL analysis. The results stress that adoption of Internet-based marketing is influenced by willingness to cannibalize, management support, market pressure, and a firm=s knowledge of IT. Willingness to cannibalize mediates the effects of future market orientation, ownership......, specialised investments, and management support. This is mainly the case in small firms, as the importance of the various drivers and the adoption pattern vary between size categories. Market-pull factors have relatively more explanatory power in medium-sized and large companies....

  14. Counseling Adopted Persons in Adulthood: Integrating Practice and Research

    Science.gov (United States)

    Baden, Amanda L.; O'Leary Wiley, Mary

    2007-01-01

    For the past 50 years, adults who were adopted during infancy have been research participants for empirical studies with goals ranging from twin studies for heritability, to adjustment following adoption, to attachment. While the research body is broad, it has given little attention to counseling practices with adopted adults. Because empirical…

  15. Development and Adjustment of Adopted Adolescents : Longitudinal and Concurrent Factors

    NARCIS (Netherlands)

    Jaffari-Bimmel, Nicole

    2005-01-01

    The first study of this thesis (Chapter 2) showed that the majority of the internationally adopted children are well adjusted, although a relatively large minority of adopted children had behavior problems of clinical significance or were referred to mental health services compared with non-adopted

  16. Factors leading to inflation targeting : The impact of adoption

    NARCIS (Netherlands)

    Samarina, Anna; Sturm, Jan-Egbert

    2013-01-01

    This paper examines how the analysis of inflation targeting (IT) adoption is affected by allowing for a structural change after adoption, using panel probit models for 60 countries over the period 1985-2008. Our findings suggest that there is a structural change after IT adoption. Including the post

  17. Stepwise innovation adoption : a neglected concept in innovation research

    NARCIS (Netherlands)

    Huizingh, K.R.E.; Brand, M.J.

    2009-01-01

    Most innovation researchers tend to consider innovation adoption as a binary process, implying that companies have either adopted an innovation or not. In this paper we focus on e-commerce as an innovation that can be adopted stepwise. We distinguish between two levels of e-commerce, basic and advan

  18. Real Parents, Real Children: Parenting the Adopted Child.

    Science.gov (United States)

    van Gulden, Holly; Bartels-Rabb, Lisa M.

    Parenting an adopted child is, for the most part, the same as parenting any other child, but is different in some unique and critical ways related to the child's separation from birth parents and genetic roots. Understanding how a child interprets, understands, and feels about adoption, and why, can help the parent guide the adopted child…

  19. Our Own: Adopting and Parenting the Older Child.

    Science.gov (United States)

    Maskew, Trish

    Children adopted when they are older come with histories, fully formed personalities, and intense anger over what they have lost. Drawing on stories of families who have adopted older children, as well as research, interviews with professionals, and opinions of adults who were adopted as children, this book explores the joys and challenges of…

  20. Post-Adoption Depression: Clinical Windows on an Emerging Concept

    Science.gov (United States)

    Speilman, Eda

    2011-01-01

    In recent years, the concept of post-adoption depression--with both parallels and differences from postpartum depression--has emerged as a salient descriptor of the experience of a significant minority of newly adoptive parents. This article offers a clinical perspective on post-adoption depression through the stories of several families seen in…

  1. Impact of the economic recession on companion animal relinquishment, adoption, and euthanasia: a Chicago animal shelter's experience.

    Science.gov (United States)

    Weng, Hsin-Yi; Hart, Lynette A

    2012-01-01

    This study investigated how the current economic recession (since December 2007) has affected dog and cat relinquishment, adoption, and euthanasia at the Anti-Cruelty Society animal shelter in Chicago, Illinois. The study compared temporal patterns of the investigated statistics before (2000-2007) the start of the current recession with the patterns after the start of the recession (2008-2010). The results showed that once the guardianship (ownership) of a nonhuman animal had been established, the recession did not greatly affect the owner's decision on relinquishment-except for the relinquishment of senior dogs, which may be associated with increased costs of care. However, an unfavorable economic environment may have reduced adoption of animals. The consequences of a decline in adoptions might be reflected in an increase in the proportion or number of sheltered animals euthanized. This study demonstrated how monitoring changes in temporal patterns in these shelter statistics can help guide animal shelters to better prepare for the current recession.

  2. Foam patterns

    Science.gov (United States)

    Chaudhry, Anil R; Dzugan, Robert; Harrington, Richard M; Neece, Faurice D; Singh, Nipendra P; Westendorf, Travis

    2013-11-26

    A method of creating a foam pattern comprises mixing a polyol component and an isocyanate component to form a liquid mixture. The method further comprises placing a temporary core having a shape corresponding to a desired internal feature in a cavity of a mold and inserting the mixture into the cavity of the mold so that the mixture surrounds a portion of the temporary core. The method optionally further comprises using supporting pins made of foam to support the core in the mold cavity, with such pins becoming integral part of the pattern material simplifying subsequent processing. The method further comprises waiting for a predetermined time sufficient for a reaction from the mixture to form a foam pattern structure corresponding to the cavity of the mold, wherein the foam pattern structure encloses a portion of the temporary core and removing the temporary core from the pattern independent of chemical leaching.

  3. Geodesic patterns

    KAUST Repository

    Pottmann, Helmut

    2010-07-26

    Geodesic curves in surfaces are not only minimizers of distance, but they are also the curves of zero geodesic (sideways) curvature. It turns out that this property makes patterns of geodesics the basic geometric entity when dealing with the cladding of a freeform surface with wooden panels which do not bend sideways. Likewise a geodesic is the favored shape of timber support elements in freeform architecture, for reasons of manufacturing and statics. Both problem areas are fundamental in freeform architecture, but so far only experimental solutions have been available. This paper provides a systematic treatment and shows how to design geodesic patterns in different ways: The evolution of geodesic curves is good for local studies and simple patterns; the level set formulation can deal with the global layout of multiple patterns of geodesics; finally geodesic vector fields allow us to interactively model geodesic patterns and perform surface segmentation into panelizable parts. © 2010 ACM.

  4. Awareness, persuasion, and adoption: Enriching the Bass model

    Science.gov (United States)

    Colapinto, Cinzia; Sartori, Elena; Tolotti, Marco

    2014-02-01

    In the context of diffusion of innovations, we propose a probabilistic model based on interacting populations connected through new communication channels. The potential adopters are heterogeneous in the connectivity levels and in their taste for innovation. The proposed framework can model the different stages of the adoption dynamics. In particular, the adoption curve is the result of a micro-founded decision process following the awareness phase. Eventually, we recover stylized facts pointed out by the extant literature in the field, such as delayed adoptions and non-monotonic adoption curves.

  5. Adoption in rock and white-tailed ptarmigan

    Science.gov (United States)

    Wong, M.M.L.; Fedy, B.C.; Wilson, S.; Martin, K.M.

    2009-01-01

    Reports of adoption in birds are widespread, but few studies report rates of adoption or possible mechanisms for this phenomenon, particularly in the Order Galliformes. We report incidents of adoption in Rock Ptarmigan (Lagopus muta) and White-tailed Ptarmigan (L. leucura) from two sites in western Canada. Adoption rates for White-tailed Ptarmigan on Vancouver Island, British Columbia, and the Ruby Ranges, Yukon Territory were 13% (n = 16 broods) and 4% (n = 27), respectively, while rates for Rock Ptarmigan were 14% (n = 29) in the Ruby Ranges. Low brood densities may result in lower rates of adoption for ptarmigan. ?? 2009 The Wilson Ornithological Society.

  6. Adoption as a Social Marker: The Diffusion of Products in a Multigroup Environment

    CERN Document Server

    Smaldino, Paul E; Hillis, Vicken; Bednar, Jenna

    2015-01-01

    Social identities are among the key factors driving social behavior in complex societies. Recent attention to social identity in consumer behavior indicates that individuals avoid products that might signal membership in an outgroup. Yet the population-level consequences of adoption as identity signaling are largely unknown. Whereas previous work has focused on asymmetric attraction and repulsion among groups with different social identities, here we consider the spread of innovations in a structured population in which there are multiple groups who don't want to be mistaken for one another, using both analytical and agent-based modeling. This formal analysis, the first to take the spatial structure of a population into account, allows us to consider empirically important factors, including demographic skew and communication scale, that likely influence overall patterns of adoption. We find that as products become emergent social markers, aversion to outgroup-associated products can decrease overall patterns ...

  7. Adopters, testers or pseudo-adopters? Dynamics of the use of improved tree fallows by farmers in western Kenya

    NARCIS (Netherlands)

    Kiptot, E.; Hebinck, P.G.M.; Franzel, S.; Richards, P.

    2007-01-01

    Although there has been increasing research on the adoption of agroforestry technologies over the last decade, few such studies have assessed uptake over a long period and many are based on a single snapshot in time. Furthermore, most of these studies have mainly looked at non-adopters and adopters:

  8. CORRECTIVE FEEDBACKS INTERACTION IN CLT-ADOPTED CLASSROOMS

    Directory of Open Access Journals (Sweden)

    Ayu Liskinasih

    2016-07-01

    Full Text Available This case study aimed to examine corrective feedback (CF pattern in the interactions of Indonesian EFL (English as Foreign Language classrooms (a speaking and a grammar classrooms which adopt CLT (Communicative Language Teaching. Two lecturers and twenty undergraduate English department students of an A-class university in Indonesia were involved as research participants. The findings revealed that the lecturers employed all types of CF to treat all types of errors. Explicit corrections were dominant in Speaking class as well as other explicit CF; whereas reformulations and prompt were equally distributed. Elicitation was dominant in Grammar class as well as other prompts; meanwhile, explicit and implicit CFs had similar proportion. The lecturers’ preferences were based on their beliefs on how their students learn foreign language and some factors such as the importance of CF to the instructional focus of the lesson, the possibility to generate student’s uptake, and also their empathetic values about students’ current language development. It was concluded that the provisions of CF in EFL classrooms reflect the application of CLT.

  9. Determinants of Adoption of Improved Onion Variety in Bangladesh

    Directory of Open Access Journals (Sweden)

    Asif Reza Anik

    2015-06-01

    Full Text Available Through exploring data collected from 300 Bangladeshi onion growers this article suggests options for widespread adoption of modern onion varieties. Cultivation of modern variety enables farmers to produce more and earn higher profit than the traditional variety growing farmers, though farmers requires more capital for cultivating modern variety. Quality of extension service and access to credit are two of the most important factors to contribute in adoption. Farmers practicing crop diversification are more likely to adopt, but when they adopt they devote relatively lower share of their available land for modern onion varieties since they are characterized to cultivate different crops. With increasing off-farm income farmers tend to adopt less. Level of adoption is likely to be higher among the experienced and educated farmers. Number of fragmented land reduces adoption probabilities.

  10. Picturing Adoption of Below-Ground Biodiversity Technologies among Smallholder Farmers around Mabira Forest, Uganda

    Directory of Open Access Journals (Sweden)

    Isabirye, BE.

    2010-01-01

    Full Text Available Faced with a multitude of soil and water amendment technologies, farmers have the task of choosing the technologies to adopt for ensuring subsistence and income sustainability. In 2008, a study to characterize the farmers was conducted around Mabira Forest, to assess the adoption of soil technologies fostering Belowground Biodiversity (BGBD. Eighty-four households (38 participating and 46 non-participants from four villages were randomly selected and interviewed. Results showed that the adoption pattern was significantly driven by farm size, labor, household size, age and wealth status of the house. Also important were farm location, gender of household head, primary occupation, soil and water conservation technologies training, land tenure, and social capital. For the few current adopters, there was a perceived increase in labor demand but overall productivity was higher, partly resulting from increased crop productivity due to soil fertility enhancement and soil structure modification. It is therefore concluded that, around Mabira forest, BGBD technologies will be adopted by farming households with sufficient land, labor and social capital.

  11. Search Patterns

    CERN Document Server

    Morville, Peter

    2010-01-01

    What people are saying about Search Patterns "Search Patterns is a delight to read -- very thoughtful and thought provoking. It's the most comprehensive survey of designing effective search experiences I've seen." --Irene Au, Director of User Experience, Google "I love this book! Thanks to Peter and Jeffery, I now know that search (yes, boring old yucky who cares search) is one of the coolest ways around of looking at the world." --Dan Roam, author, The Back of the Napkin (Portfolio Hardcover) "Search Patterns is a playful guide to the practical concerns of search interface design. It cont

  12. Chladni Pattern

    OpenAIRE

    Xiao, Wence

    2010-01-01

    The report aimed at mathematically describing the Chladni Pattern for a plate based on the equation of motion in two dimensions. It began with deriving the equation of motion of a single point from Newton’s laws, and later derived the equation of motion for the vibration in two dimension, and gave the normal modes through the method of separation of variables. The next was deriving the nodal pattern when the initial conditions and bound- ary conditions were given. At t...

  13. Government Should Adopt Policies Favouring Rural Migrants

    Institute of Scientific and Technical Information of China (English)

    韩长赋

    2007-01-01

    Based on a systematic analysis of the development trends of Chinese rural migrant workers, this article has designed a model representing the flow of migrant workers from rural to urban areas, and the patterns of urban settlement. The significant factors contributing to the problems of migrant workers are assessed and suggestions for improvement put forward. This article concludes that the rural migrant worker problem is attributable to institutional set-up and strategy and will continue for the entire process of China’s modernization. It is estimated that modernization will be realized in about 2050, at which time the rural-urban migration process will also be completed. To solve the problems of migrant workers, a comprehensive strategy of balanced development between urban and rural areas should be carried out, featuring industry’s support of agriculture and urban support of rural areas. Furthermore, the urban-rural dual structure should be gradually eliminated through institutional reform and policy innovation. The principles of equality, humanity and fair treatment for both urban and rural populations should be sought.

  14. Adoption and Abandonment of Dual Exchange Rate Systems Adoption and Abandonment of Dual Exchange Rate Systems

    Directory of Open Access Journals (Sweden)

    Jose Saul Lizondo

    1990-03-01

    Full Text Available Adoption and Abandonment of Dual Exchange Rate Systems When facing persistent balance of payments problems, some countries have resorted to a dual exchange rate system as an alternative to a uniform exchange rate adjustment. Typically, under the dual system, certain selected transactions take place at a fixed official exchange rate, while there remaining transactions are effected at a more depreciated rate, which is usually determined by market forces. This paper examines the circumstances that lead to the adoption of a dual system, and the conditions under which the foreign exchange market can be unified successfully or a later stage. In this paper, the adoption of the dual system is linked to the unsustainability of a crawling peg (or a fixed exchange rate system in the presence of large budgret deficits. We show that the initiai spiral between the financial at the commercial exchange rates and the extent of capital flight largely depends on whether the switch in regime is anticipated or unanticipated. Although the dual system improves the external position of the economy, to the extent that there is no change in domestic noticies the country will continue to experience a deficit in the balance of payments. A correction of these policies is the only enduring solution to the external imbalance, and a precondition for a successful unification of the foreirg exchange market. If the economv unifies the foreign exchange market into a crawling peg, the financial exchahnge rate could be an adequate indicator of the initlal level of which the new exchange rate should be set if a capital outflow is to be avoided. If the economy instead moves to a flexible exchange rate system, the initial value of the exchange rate could be higher or lower than the prevailing financial exchange rate.

  15. Adoption of telemedicine: from pilot stage to routine delivery

    Directory of Open Access Journals (Sweden)

    Zanaboni Paolo

    2012-01-01

    Full Text Available Abstract Background Today there is much debate about why telemedicine has stalled. Teleradiology is the only widespread telemedicine application. Other telemedicine applications appear to be promising candidates for widespread use, but they remain in the early adoption stage. The objective of this debate paper is to achieve a better understanding of the adoption of telemedicine, to assist those trying to move applications from pilot stage to routine delivery. Discussion We have investigated the reasons why telemedicine has stalled by focusing on two, high-level topics: 1 the process of adoption of telemedicine in comparison with other technologies; and 2 the factors involved in the widespread adoption of telemedicine. For each topic, we have formulated hypotheses. First, the advantages for users are the crucial determinant of the speed of adoption of technology in healthcare. Second, the adoption of telemedicine is similar to that of other health technologies and follows an S-shaped logistic growth curve. Third, evidence of cost-effectiveness is a necessary but not sufficient condition for the widespread adoption of telemedicine. Fourth, personal incentives for the health professionals involved in service provision are needed before the widespread adoption of telemedicine will occur. Summary The widespread adoption of telemedicine is a major -- and still underdeveloped -- challenge that needs to be strengthened through new research directions. We have formulated four hypotheses, which are all susceptible to experimental verification. In particular, we believe that data about the adoption of telemedicine should be collected from applications implemented on a large-scale, to test the assumption that the adoption of telemedicine follows an S-shaped growth curve. This will lead to a better understanding of the process, which will in turn accelerate the adoption of new telemedicine applications in future. Research is also required to identify

  16. Process innovation objectives and management complementarities: patterns, drivers, co-adoption and performance effects

    OpenAIRE

    Hervas-Oliver, J.L.; Sempere-Ripoll, F.; Boronat-Moll, C.

    2012-01-01

    The excessive concentration of the innovation literature on product development, its drivers and effects, has almost neglected an important strategy which develops and sustains a firm's competitive advantage: process development or innovation. This is an examination of process innovation as more than a mere dependent variable for predicting innovators. It provides insights into the poor attention that process innovation variable has received as an indicator of a firm's performance. In additio...

  17. Adopting Workflow Patterns for Modelling the Allocation of Data in Multi-Organizational Collaborations

    NARCIS (Netherlands)

    Kassahun, A.; Tekinerdogan, B.

    2016-01-01

    Currently, many organizations need to collaborate to achieve their common objectives. An important aspect hereby is the data required for making decisions at anyone organization may be distributed over the different organizations involved in the collaboration. The data objects and the activities in

  18. The Cloud Adoption Toolkit: Supporting Cloud Adoption Decisions in the Enterprise

    CERN Document Server

    Khajeh-Hosseini, Ali; Smith, James W; Sommerville, Ian

    2010-01-01

    Cloud computing promises a radical shift in the provisioning of computing resource within the enterprise. This paper describes the challenges that decision makers face when assessing the feasibility of the adoption of cloud computing in their organisations, and describes our Cloud Adoption Toolkit, which has been developed to support this process. The toolkit provides a framework to support decision makers in identifying their concerns, and matching these concerns to appropriate tools/techniques that can be used to address them. Cost Modeling is the most mature tool in the toolkit, and this paper shows its effectiveness by demonstrating how practitioners can use it to examine the costs of deploying their IT systems on the cloud. The Cost Modeling tool is evaluated using a case study of an organization that is considering the migration of some of its IT systems to the cloud. The case study shows that running systems on the cloud using a traditional "always on" approach can be less cost effective, and the elast...

  19. Mandatory IFRS adoption and executive compensation: Evidence from China

    Directory of Open Access Journals (Sweden)

    Qingchuan Hou

    2014-03-01

    Full Text Available This study investigates how the mandatory adoption of International Financial Reporting Standards (IFRS affects the contractual benefits of using accounting information to determine executive compensation in China. After controlling for firm and corporate governance characteristics, we find strong evidence supporting the positive role of mandatory IFRS adoption on the accounting-based performance sensitivity of executive compensation. Subsample analysis suggests that improvements in accounting-based performance sensitivity after IFRS adoption differ across regions with various levels of institutional quality and across firms that are affected to a different extent by the adoption. Additional analysis supports the argument that the positive effects of IFRS adoption on the use of accounting performance in executive compensation are driven by the reduction in accounting conservatism associated with IFRS adoption.

  20. Mandatory IFRS adoption and executive compensation:Evidence from China

    Institute of Scientific and Technical Information of China (English)

    Qingchuan; Hou; Qinglu; Jin; Lanfang; Wang

    2014-01-01

    This study investigates how the mandatory adoption of International Financial Reporting Standards(IFRS) affects the contractual benefits of using accounting information to determine executive compensation in China. After controlling for firm and corporate governance characteristics, we find strong evidence supporting the positive role of mandatory IFRS adoption on the accounting-based performance sensitivity of executive compensation. Subsample analysis suggests that improvements in accounting-based performance sensitivity after IFRS adoption differ across regions with various levels of institutional quality and across firms that are affected to a different extent by the adoption. Additional analysis supports the argument that the positive effects of IFRS adoption on the use of accounting performance in executive compensation are driven by the reduction in accounting conservatism associated with IFRS adoption.

  1. Some Considerations on the Adoption Registration in the Civil Registry

    Directory of Open Access Journals (Sweden)

    Gabriela LUPŞAN

    2014-12-01

    Full Text Available Filiation is, in a broad sense, an identity element, dependent or not on the biological relationships, that represents, in some situations, either a condition for the existence of a right (e.g. the right to inheritance or for example to conclude a legal act (e.g. marriage. The proof of filiation is the birth certificate drawn up in civil registry or on the material basis of birth, or on the basis of the adoption judgment. In this paper, we aimed at analyzing the final part of the adoption procedure, the subsequent stage for becoming final the adoption judgment, which sets face to face the adopting person or family and the administrative authority, obliged to execute the judge's decision, i.e. to create a filiation relation between the adopted on the one hand and the adoptive parent or parents.

  2. Optimal signal patterns for dynamical cellular communication

    CERN Document Server

    Hasegawa, Yoshihiko

    2015-01-01

    Cells transmit information via signaling pathways, using temporal dynamical patterns. As optimality with respect to environments is the universal principle in biological systems, organisms have acquired an optimal way of transmitting information. Here we obtain optimal dynamical signal patterns which can transmit information efficiently (low power) and reliably (high accuracy) using the optimal control theory. Adopting an activation-inactivation decoding network, we reproduced several dynamical patterns found in actual signals, such as steep, gradual and overshooting dynamics. Notably, when minimizing the power of the input signal, optimal signals exhibit the overshooting pattern, which is a biphasic pattern with transient and steady phases; this pattern is prevalent in actual dynamical patterns as it can be generated by an incoherent feed-forward loop (FFL), a common motif in biochemical networks. We also identified conditions when the three patterns, steep, gradual and overshooting, confer advantages.

  3. Optimal temporal patterns for dynamical cellular signaling

    Science.gov (United States)

    Hasegawa, Yoshihiko

    2016-11-01

    Cells use temporal dynamical patterns to transmit information via signaling pathways. As optimality with respect to the environment plays a fundamental role in biological systems, organisms have evolved optimal ways to transmit information. Here, we use optimal control theory to obtain the dynamical signal patterns for the optimal transmission of information, in terms of efficiency (low energy) and reliability (low uncertainty). Adopting an activation-deactivation decoding network, we reproduce several dynamical patterns found in actual signals, such as steep, gradual, and overshooting dynamics. Notably, when minimizing the energy of the input signal, the optimal signals exhibit overshooting, which is a biphasic pattern with transient and steady phases; this pattern is prevalent in actual dynamical patterns. We also identify conditions in which these three patterns (steep, gradual, and overshooting) confer advantages. Our study shows that cellular signal transduction is governed by the principle of minimizing free energy dissipation and uncertainty; these constraints serve as selective pressures when designing dynamical signaling patterns.

  4. Associations between Discussions of Racial and Ethnic Differences in Internationally Adoptive Families and Delinquent Behavior among Korean Adopted Adolescents.

    Science.gov (United States)

    Anderson, Kayla N; Lee, Richard M; Rueter, Martha A; Kim, Oh Myo

    2015-04-01

    Internationally adopted adolescents may have more delinquent behavior than non-adopted adolescents. One explanation is these adolescents experience discrimination and loss of culture, and adoptive parents are not adequately addressing these experiences. However, studies have not examined the effects of family discussions of racial and ethnic differences within adoptive families on adopted adolescents' delinquent behavior. To test this relationship, this study utilized data from 111 U.S. internationally adoptive families with 185 South Korean adopted adolescents (55% female, M age = 17.75). During an observational assessment, families discussed the importance of their racial and ethnic differences, and adolescents completed a delinquent behavior questionnaire. Analysis of covariance showed differences in adolescent delinquent behavior across three ways adoptive families discussed racial and ethnic differences; adolescents whose families acknowledged differences had the fewest mean delinquent behaviors. There were no significant differences in delinquent behavior between adolescents whose families acknowledged or rejected the importance of racial and ethnic differences. However, adopted adolescents whose families held discrepant views of differences had significantly more problem behavior than adolescents whose families either acknowledged or rejected the importance of racial and ethnic differences. Clinicians, adoption professionals, and other parenting specialists should focus on building cohesive family identities about racial and ethnic differences, as discrepant views of differences are associated with the most adoptee delinquent behavior.

  5. Management Challenges for DevOps Adoption within UK SMEs

    OpenAIRE

    Jones, Stephen; Noppen, Joost; Lettice, Fiona

    2016-01-01

    The DevOps phenomenon is gathering pace as more UK organisations seek to leverage the benefits it can potentially bring to software engineering functions. However substantial organisational change is inherent to adopting DevOps, especially where there are prior and established methods. As part of a wider piece of doctoral research investigating the management challenges of DevOps adoption, we present early findings of a six month qualitative diary study following the adoption of DevOps within...

  6. Assortative mating by unwed biological parents of adopted children.

    Science.gov (United States)

    Plomin, R; DeFries, J C; Roberts, M K

    1977-04-22

    Analyses of data obtained from 662 unwed couples whose children were relinquished for adoption reveal that biological parents of adopted children mate assortatively. For physical characters, assortative mating of unwed parents was similar to that of wed parents; for behavior characters, however, there was less assortative mating by the unwed parents. Because assortative mating inflates estimates of genetic parameters in adoption studies, future studies should collect information on both biological parents.

  7. Critical Factors for Personal Cloud Storage Adoption in China

    OpenAIRE

    Jianya Wang

    2016-01-01

    Purpose: In order to explain and predict the adoption of personal cloud storage, this study explores the critical factors involved in the adoption of personal cloud storage and empirically validates their relationships to a user's intentions. Design/methodology/approach: Based on technology acceptance model (TAM), network externality, trust, and an interview survey, this study proposes a personal cloud storage adoption model. We conducted an empirical analysis by structural equation modeli...

  8. Transracial adoption: the conflict in the dialogical self

    OpenAIRE

    2008-01-01

    The aim of this study is to investigate, through individual narratives, the conflicting voices in the ‘dialogical self’ of transracially adopting mothers. It is hypothesised that internalised voices from the family of origin are in conflict with the interracial voice of the new interracial family. The exchange or conflict between the voices is said to influence the transracially adopting mother’s experience and understanding of transracial adoption. The literature review of chapter two compri...

  9. Essays on Technology Adoption and Provision Efficiency in Development Projects

    OpenAIRE

    2016-01-01

    In many development projects, technologies with potential to improve welfare are under adopted and under-utilized once adopted. One possible cause for this inefficiency is ignoring fit risk (the risk the technology may not meet the needs of the potential adopter). This dissertation attempts to introduce fit risk in the development context and develops a framework to analyze resource misallocation when fit risk is not addressed by project donors and managers. We examine strategies to reduce fi...

  10. Crossing the Technology Adoption Chasm: Implications for DoD

    Science.gov (United States)

    2008-06-30

    Computer-aided Software Engineering (CASE) .......................................14 Health Technology Assessments ( HTA ...the Innovators, the Early Adopters, the Early Majority, the Late Majority, and finally the Laggards. The “Technology Adoption Chasm” refers to the...gap in the TAL between the Early Adopters and the Early Majority and is sometimes referred to as the “Valley of Death” (VoD), particularly within the

  11. Pattern Formation

    Science.gov (United States)

    Hoyle, Rebecca

    2006-03-01

    From the stripes of a zebra and the spots on a leopard's back to the ripples on a sandy beach or desert dune, regular patterns arise everywhere in nature. The appearance and evolution of these phenomena has been a focus of recent research activity across several disciplines. This book provides an introduction to the range of mathematical theory and methods used to analyse and explain these often intricate and beautiful patterns. Bringing together several different approaches, from group theoretic methods to envelope equations and theory of patterns in large-aspect ratio-systems, the book also provides insight behind the selection of one pattern over another. Suitable as an upper-undergraduate textbook for mathematics students or as a fascinating, engaging, and fully illustrated resource for readers in physics and biology, Rebecca Hoyle's book, using a non-partisan approach, unifies a range of techniques used by active researchers in this growing field. Accessible description of the mathematical theory behind fascinating pattern formation in areas such as biology, physics and materials science Collects recent research for the first time in an upper level textbook Features a number of exercises - with solutions online - and worked examples

  12. Mobile technology and healthcare: the adoption issues and systemic problems.

    Science.gov (United States)

    Standing, Susan; Standing, Craig

    2008-01-01

    Although the benefits that are associated with mobile technology have been recognised as offering great potential in the healthcare sector, its widespread adoption has been lagging. We propose that fundamental systemic issues are likely to be the main barriers to adoption. We explain that the fragmented nature of the conservative healthcare system, the contradictory incentives and improper outcome measures conspire to make the innovative adoption of mobile technology problematic. Researchers can only gain a limited understanding of a technology's potential success by using technology adoption frameworks and need to supplement this with a 'systems' perspective that takes a more strategic view.

  13. Innovation adoption: a review of theories and constructs.

    Science.gov (United States)

    Wisdom, Jennifer P; Chor, Ka Ho Brian; Hoagwood, Kimberly E; Horwitz, Sarah M

    2014-07-01

    Many theoretical frameworks seek to describe the dynamic process of the implementation of innovations. Little is known, however, about factors related to decisions to adopt innovations and how the likelihood of adoption of innovations can be increased. Using a narrative synthesis approach, this paper compared constructs theorized to be related to adoption of innovations proposed in existing theoretical frameworks in order to identify characteristics likely to increase adoption of innovations. The overall goal was to identify elements across adoption frameworks that are potentially modifiable and, thus, might be employed to improve the adoption of evidence-based practices. The review identified 20 theoretical frameworks that could be grouped into two broad categories: theories that mainly address the adoption process (N = 10) and theories that address adoption within the context of implementation, diffusion, dissemination, and/or sustainability (N = 10). Constructs of leadership, operational size and structure, innovation fit with norms and values, and attitudes/motivation toward innovations each are mentioned in at least half of the theories, though there were no consistent definitions of measures for these constructs. A lack of precise definitions and measurement of constructs suggests further work is needed to increase our understanding of adoption of innovations.

  14. THE INFLUENCE OF CONTINGENCIES ON THE ADOPTION OF ACTION PROGRAMMES

    DEFF Research Database (Denmark)

    Timenes Laugen, Bjørge; Acur, Nuran; Boer, Harry

    2008-01-01

    chain management and quality programmes, but not programmes aimed at organizational, manufacturing process or NPD improvement. The adoption of all five groups of action programmes increases with company size, which suggests company size to be the most important determining factor for the adoption...... production process types, company size, and market dynamics and competition intensity affect the adoption of manufacturing improvement programmes. Analysing data from the International Manufacturing Strategy Survey (IMSS) IV, we find that production process type seems to determine the adoption of supply...

  15. Competition of simple and complex adoption on interdependent networks

    Science.gov (United States)

    Czaplicka, Agnieszka; Toral, Raul; San Miguel, Maxi

    2016-12-01

    We consider the competition of two mechanisms for adoption processes: a so-called complex threshold dynamics and a simple susceptible-infected-susceptible (SIS) model. Separately, these mechanisms lead, respectively, to first-order and continuous transitions between nonadoption and adoption phases. We consider two interconnected layers. While all nodes on the first layer follow the complex adoption process, all nodes on the second layer follow the simple adoption process. Coupling between the two adoption processes occurs as a result of the inclusion of some additional interconnections between layers. We find that the transition points and also the nature of the transitions are modified in the coupled dynamics. In the complex adoption layer, the critical threshold required for extension of adoption increases with interlayer connectivity whereas in the case of an isolated single network it would decrease with average connectivity. In addition, the transition can become continuous depending on the detailed interlayer and intralayer connectivities. In the SIS layer, any interlayer connectivity leads to the extension of the adopter phase. Besides, a new transition appears as a sudden drop of the fraction of adopters in the SIS layer. The main numerical findings are described by a mean-field type analytical approach appropriately developed for the threshold-SIS coupled system.

  16. Why farmers adopt best management practice in the United States: A meta-analysis of the adoption literature

    Science.gov (United States)

    Baumgart-Getz, Adam; Stalker Prokopy, Linda; Floress, Kristin

    2012-01-01

    This meta-analysis of both published and unpublished studies assesses factors believed to influence adoption of agricultural Best Management Practices in the United States. Using an established statistical technique to summarize the adoption literature in the United States, we identified the following variables as having the largest impact on adoption: access to and quality of information, financial capacity, and being connected to agency or local networks of farmers or watershed groups. This study shows that various approaches to data collection affect the results and comparability of adoption studies. In particular, environmental awareness and farmer attitudes have been inconsistently used and measured across the literature. This meta-analysis concludes with suggestions regarding the future direction of adoption studies, along with guidelines for how data should be presented to enhance the adoption of conservation practices and guide research.

  17. Exploring the adoption of Schwartz Center Rounds as an organisational innovation to improve staff well-being in England, 2009–2015

    Science.gov (United States)

    Philippou, Julia; Leamy, Mary; Reynolds, Ellie; Ross, Shilpa; Bennett, Laura; Taylor, Cath; Shuldham, Caroline; Maben, Jill

    2017-01-01

    Objectives Schwartz Center Rounds (‘Rounds’) are a multidisciplinary forum in which healthcare staff within an organisation discuss the psychological, emotional and social challenges associated with their work in a confidential and safe environment. Implemented in over 375 North American organisations, since 2009, they have been increasingly adopted in England. This study aimed to establish how many and what types of organisations have adopted Rounds in England, and to explore why they did so. Setting Public healthcare organisations in England. Participants Secondary data analysis was used to map and profile all 116 public healthcare organisations that had adopted Rounds in England by July 2015. Semistructured telephone interviews were conducted with 45 Round coordinators within adopting organisations. Results The rate of adoption increased after a major national report in 2013. Rounds were typically adopted in order to improve staff well-being. Adopting organisations scored better on staff engagement than non-adopters; among adopting organisations, those performing better on patient experience were more likely to adopt earlier. Most adoption decision-making processes were straightforward. A confluence of factors—a generally favourable set of innovation attributes (including low cost), advocacy from opinion leaders in different professional networks, active dissemination by change agents and a felt need to be seen to be addressing staff well-being—initially led to Rounds being seen as ‘an idea whose time had come’. More recent adoption patterns have been shaped by the timing of charitable and other agency funding in specific geographical areas and sectors, as well as several forms of ‘mimetic pressure’. Conclusions The innate attributes of Rounds, favourable circumstances and the cumulative impact of a sequence of distinct informal and formal social processes have shaped the pattern of their adoption in England. PMID:28057662

  18. Local cascades induced global contagion: How heterogeneous thresholds, exogenous effects, and unconcerned behaviour govern online adoption spreading

    CERN Document Server

    Karsai, Márton; Kikas, Riivo; Kaski, Kimmo; Kertész, János

    2016-01-01

    Adoption of innovations, products or online services is commonly interpreted as a spreading process driven to large extent by social influence and conditioned by the needs and capacities of individuals. To model this process one usually introduces behavioural threshold mechanisms, which can give rise to the evolution of global cascades if the system satisfies a set of conditions. However, these models do not address temporal aspects of the emerging cascades, which in real systems may evolve through various pathways ranging from slow to rapid patterns. Here we fill this gap through the analysis and modelling of product adoption in the world's largest voice over internet service, the social network of Skype. We provide empirical evidence about the heterogeneous distribution of fractional behavioural thresholds, which appears to be independent of the degree of adopting egos. We show that the structure of real-world adoption clusters is radically different from previous theoretical expectations, since vulnerable ...

  19. Adoption of romanian children in The Netherlands: behavior problems and parenting burden of upbringing for adoptive parents.

    Science.gov (United States)

    Hoksbergen, René; Rijk, Kathinka; Van Dijkum, Cor; Ter Laak, Jan

    2004-06-01

    Seventy-two Dutch adoptive parents of 80 Romanian children completed the Child Behavior Checklist (CBCL), the Nijmegen Questionnaire for the Upbringing Situation (NQUS), and participated in a semistructured interview (response 83%). Thirty-four percent of the adopted children scored in the clinical range on Externalizing and 36 % on Total Problems scales. Significantly more children who were at least two years at placement and had stayed less than five years in the adoptive family received scores in the clinical range on almost all CBCL clinical clusters. Nevertheless, the adoptive parents judge their upbringing situation positive (NQUS).

  20. The Adoption of Smoking and Its Effect on the Mortality Gender Gap in Netherlands: A Historical Perspective

    NARCIS (Netherlands)

    Janssen, F.; van Poppel, F.W.A.

    2015-01-01

    We examine in depth the effect of differences in the smoking adoption patterns of men and women on the mortality gender gap in Netherlands, employing a historical perspective. Using an indirect estimation technique based on observed lung cancer mortality from1931 to 2012, we estimated lifetime smoki

  1. 24 CFR 58.52 - Adoption of other agencies' EISs.

    Science.gov (United States)

    2010-04-01

    ... RESPONSIBILITIES Environmental Review Process: Environmental Impact Statement Determinations § 58.52 Adoption of... 24 Housing and Urban Development 1 2010-04-01 2010-04-01 false Adoption of other agencies' EISs. 58.52 Section 58.52 Housing and Urban Development Office of the Secretary, Department of Housing...

  2. The Optimal Timing of Adoption of a Green Technology

    Energy Technology Data Exchange (ETDEWEB)

    Cunha-e-Sa, M.A.; Reis, A.B. [Faculdade de Economia, Universidade Nova de Lisboa, Campus de Campolide, P-1099-032 Lisbon (Portugal)

    2007-01-15

    We study the optimal timing of adoption of a cleaner technology and its effects on the rate of growth of an economy in the context of an AK endogenous growth model. We show that the results depend upon the behavior of the marginal utility of environmental quality with respect to consumption. When it is increasing, we derive the capital level at the optimal timing of adoption. We show that this capital threshold is independent of the initial conditions on the stock of capital, implying that capital-poor countries tend to take longer to adopt. Also, country-specific characteristics, as the existence of high barriers to adoption, may lead to different capital thresholds for different countries. If the marginal utility of environmental quality decreases with consumption, a country should never delay adoption; the optimal policy is either to adopt immediately or, if adoption costs are {sup t}oo high{sup ,} to never adopt. The policy implications of these results are discussed in the context of the international debate surrounding the environmental political agenda.

  3. Determinants of Social Contagion During New Product Adoption

    NARCIS (Netherlands)

    Langley, D.J.; Bijmolt, T.H.A.; Ortt, J.R.; Pals, N.

    2012-01-01

    Social contagion has been shown to play an important role during new product adoption by consumers. Social contagion is the process by which consumers influence each other to adopt and use a product in a specific way. Current literature makes a basic assumption that social contagion is caused by the

  4. Managing transitions: assuring the adoption and impact of TQM.

    Science.gov (United States)

    Kaluzny, A D; McLaughlin, C P

    1992-11-01

    Assuring the full adoption and impact of total quality management (TQM) requires the understanding that TQM involves transitional challenges to managers, individuals, work groups, and the organization as a whole. This article presents some of these challenges and describes how they might be met over time to determine the ultimate success of TQM adoption in an organization.

  5. Social Competence in Internationally Adopted and Institutionalized Children

    Science.gov (United States)

    Palacios, Jesus; Moreno, Carmen; Roman, Maite

    2013-01-01

    A growing, sizable proportion of school children do not live in conventional family environments. Among these, internationally adopted children have gained increasing visibility in recent years. While other areas of their behavior have been widely explored, little is known about adopted children's social competence and their integration into peer…

  6. Adoption subsidy versus technology standards under asymmetric information

    NARCIS (Netherlands)

    I.V. Ossokina (Ioulia); O.H. Swank (Otto)

    2008-01-01

    textabstractMarket-based instruments are believed to create more efficient incentives for firms to adopt new technologies than command-and-control policies. We compare the effects of a direct technology regulation and of an adoption subsidy under asymmetric information about the costs of technologic

  7. 23 CFR 1210.4 - Adoption of zero tolerance law.

    Science.gov (United States)

    2010-04-01

    ... 23 Highways 1 2010-04-01 2010-04-01 false Adoption of zero tolerance law. 1210.4 Section 1210.4... TRANSPORTATION GUIDELINES OPERATION OF MOTOR VEHICLES BY INTOXICATED MINORS § 1210.4 Adoption of zero tolerance... that license suspensions or revocations are authorized for any violation of the State zero...

  8. Understanding ICT Adoption Determinants among Indonesian SMEs in Fashion Subsector

    Directory of Open Access Journals (Sweden)

    Rini Setiowati

    2015-04-01

    Full Text Available This paper investigates factors that affect the adoption of Information Communication and Technology (ICT among SMEs in fashion subsector in Indonesia. This study adopts Technology, Organization, and Environment (TOE framework which consist technological factors such as relative advantage, compatibility, complexity, trialability, observability; organizational factors such as owner/manager IT knowledge, owner/manager innovativeness;and environmental factors namely competitive pressure, market turbulence, and institutional intervention. A questionnaire-based survey was used to collect data from 204 SME owners/managers. The results show that under technological factors, compatibility, complexity, trialability, and observability significantly and positively influence ICT adoption. However, relative advantage and complexity have significant but negative influence on ICT adoption. Moreover, the organizational factors namely owner/manager’s knowledge and innovativeness also contribute significantly to the adoption. Furthermore, among three environmental factors, competitive pressure and institutional intervention are determinants that influence ICT adoption in SMEs, whereas market turbulence shows no significant contribution toward the adoption of ICT. This study provides valuable insights for government and policy makers as well as for SMEs owner/managers to develop strategies that promote and foster the adoption of ICT.

  9. 38 CFR 3.58 - Child adopted out of family.

    Science.gov (United States)

    2010-07-01

    ... 38 Pensions, Bonuses, and Veterans' Relief 1 2010-07-01 2010-07-01 false Child adopted out of family. 3.58 Section 3.58 Pensions, Bonuses, and Veterans' Relief DEPARTMENT OF VETERANS AFFAIRS... out of family. A child of a veteran adopted out of the family of the veteran either prior...

  10. Demand Heterogeneity and the Adoption of Platform Complements

    NARCIS (Netherlands)

    J. Rietveld (Joost); J.P. Eggers

    2016-01-01

    textabstractThis paper offers a demand-based theory of how platform maturity affects the adoption of platform complements. We argue that differences between early and late adopters of the platform include willingness to pay for the platform-and-complement bundle, risk preferences, preference for nov

  11. ATM Technology Adoption in U.S. Campus Networking.

    Science.gov (United States)

    Yao, Engui; Perry, John F.; Anderson, Larry S.; Brook, R. Dan; Hare, R. Dwight; Moore, Arnold J.; Xu, Xiaohe

    This study examined the relationships between ATM (asynchronous transfer mode) adoption in universities and four organizational variables: university size, type, finances, and information processing maturity. Another purpose of the study was to identify the current status of ATM adoption in campus networking. Subjects were university domain LAN…

  12. Benefits and Financial Impacts of Adopting Technology in Learning

    Science.gov (United States)

    Grenman, Katri; Isomursu, Minna; Federley, Maija; Seisto, Anu

    2013-01-01

    This paper summarizes the results of an analysis of the impacts of adopting information and communication technology (ICT) solutions in a learning context. The analysis is based on a literature survey of articles reporting research cases studying the impact of adopting ICT based solutions in various learning contexts. The subject has been reviewed…

  13. Categories for Barriers to Adoption of Instructional Technologies

    Science.gov (United States)

    Reid, Pat

    2014-01-01

    Although higher education has spent millions of dollars on instructional technologies, often higher education administration complains that instructors are not adopting them. Without a full understanding of possible barriers, higher education institutes are hard-pressed to develop either appropriate goals or sound strategies for the adoption of…

  14. 45 CFR Appendix B to Part 1355 - Adoption Data Elements

    Science.gov (United States)

    2010-10-01

    ... to build or maintain satisfactory interpersonal relationships; inappropriate types of behavior or... Applicable)___ Yes: 1 No: 2 Unable to Determine: 3 D. Relationship of Adoptive Parent(s) to the Child.... D. Relationship to Adoptive Parent(s)—Indicate the prior relationship(s) the child had with...

  15. Factors Influencing Cloud-Computing Technology Adoption in Developing Countries

    Science.gov (United States)

    Hailu, Alemayehu

    2012-01-01

    Adoption of new technology has complicating components both from the selection, as well as decision-making criteria and process. Although new technology such as cloud computing provides great benefits especially to the developing countries, it has challenges that may complicate the selection decision and subsequent adoption process. This study…

  16. Catalyzing new product adoption at the base of the pyramid

    NARCIS (Netherlands)

    Marinakis, Y.D.; Walsh, S.T.; Harms, R.

    2016-01-01

    One of the more perplexing of the entrepreneurial issues at the Base of the Pyramid (BoP) is how to catalyze new product adoption by BoP consumers. Because S-shaped adoption dynamics are the result of cultural transmission bias, the question can be rephrased as, how can an entrepreneur overcome conf

  17. Assessing the Role of Technology Adoption in China's Growth Performance

    DEFF Research Database (Denmark)

    Wirz, Nadja

    to what extent these productivity increases can be explained by technology-adoption theory. In less developed countries, the key element behind technological progress is technology adoption, the process of copying technological knowledge invented throughout the world. To uncover a measure of China...

  18. Understanding the role of social capital in adoption decisions

    NARCIS (Netherlands)

    Hunecke, Claudia; Engler, Alejandra; Jara-Rojas, Roberto; Poortvliet, Marijn

    2017-01-01

    Recently, social capital has gained importance in explaining technology adoption decisions by farmers. In this paper, we examine the impact of social capital on the adoption of irrigation technology and irrigation scheduling among wine producers in Central Chile. We propose three hypotheses: that

  19. 8 CFR 204.311 - Convention adoption home study requirements.

    Science.gov (United States)

    2010-01-01

    ..., or began a home study process in relation to an adoption or to any form of foster or other custodial... any specific restrictions to the adoption based on the age or gender, or other characteristics of the... certification must also specify the basis under 22 CFR part 96 (public domestic authority, accredited...

  20. Determinants of social contagion during new product adoption

    NARCIS (Netherlands)

    Langley, D.J.; Bijmolt, T.H.A.; Ortt, J.R.; Pals, N.

    2012-01-01

    Social contagion has been shown to play an important role during new product adoption by consumers. Social contagion is the process by which consumers influence each other to adopt and use a product in a specific way. Current literature makes a basic assumption that social contagion is caused by the

  1. "School Adopts an Experiment": The Magnetic Levitation of Superconductors

    Science.gov (United States)

    Gallitto, Aurelio Agliolo

    2010-01-01

    The event "School adopts an experiment" is an event targeted at high schools and secondary schools. It is based on a tight and direct collaboration between researchers and school students and teachers. Several schools were involved in the event by "adopting" an experiment in physics research laboratories. Groups of selected students were first…

  2. Higher Education Sub-Cultures and Open Source Adoption

    Science.gov (United States)

    van Rooij, Shahron Williams

    2011-01-01

    Successful adoption of new teaching and learning technologies in higher education requires the consensus of two sub-cultures, namely the technologist sub-culture and the academic sub-culture. This paper examines trends in adoption of open source software (OSS) for teaching and learning by comparing the results of a 2009 survey of 285 Chief…

  3. Factors leading to inflation targeting - the impact of adoption

    NARCIS (Netherlands)

    Samarina, Anna; Sturm, Jan-Egbert

    2013-01-01

    This paper examines how the analysis of inflation targeting (IT) adoption is affected by the choice of the analyzed period. We test whether country characteristics influence the decision to apply IT differently before and after its adoption, using panel probit models for 60 countries over the period

  4. The Mother-Infant Attachment Process in Adoptive Families.

    Science.gov (United States)

    Portello, Jacqueline Y.

    1993-01-01

    Reviews literature on attachment process between adopted children and their mothers. Explains empirical research in relation to Bowlby's theoretical formulation of attachment, basic thesis of which is that infants develop affectional bonds at specific ages of their development and, therefore, child's age at time of adoption is important…

  5. Adoption and perspectives of precision farming in Denmark

    DEFF Research Database (Denmark)

    Pedersen, Søren Marcus; Fountas, Spyros; Blackmore, Simon;

    2004-01-01

    Precision farming (PF) and site-specific input application based on GPS has been a management tool and option for arable farmers for about 10 years. About 400 Danish farmers have already adopted some PF-practices on their farms. This adoption and the technical and economic perspectives of PF have...

  6. Parenting Stress in Families Adopting Children from Romanian Orphanages.

    Science.gov (United States)

    Mainemer, Henry; Gilman, Lorraine C.; Ames, Elinor W.

    1998-01-01

    Parenting stress was found to be greater in families that adopted children who had been institutionalized for eight months or more in a Romanian orphanage than those in two comparison groups. Aspects of child behavior and family factors which contributed to stress, and implications for special needs adoptions are discussed. (Author/EMK)

  7. Adopting and Evaluating Service Oriented Architecture in Industry

    NARCIS (Netherlands)

    Nasr, K.A.; Gross, H.; Van Deursen, A.

    2006-01-01

    In this paper, we present a descriptive case study covering the re-engineering and furhter evolution of adopting service oriented architecture (SOA) in industry. The goal of this case study is to identify the possible benefits and bottlenecks of adopting SOA, as well as to come up with best practice

  8. Monitoring techniques in the capture and adoption of dogs and cats

    Directory of Open Access Journals (Sweden)

    Jason Onell Ardila Galvis

    2015-11-01

    Full Text Available The continuous improvement of the information systems of organizations that work toward the control of stray dog and cat populations facilitates the implementation of programs aimed at reducing the number of animals that roam free in public streets. This study aimed to present techniques to improve the understanding of the spatial distribution of stray dogs and cats and of people who adopt these animals and the fate of these animals in zoonosis control centers (ZCC. Ripley’s K function was used with a Euclidean distance graph to detect the distribution pattern of dogs and cats captured and of the people who adopted these animals. An estimate of the kernel density was used to allow a better assessment of the spatial distribution of the phenomenon studied. The results showed that the distribution of captured animals and of the people who adopted these animals form a spatial cluster (p = 0.01. Most of the animals were captured near the premises of the ZCC and near the downtown area. Factors such as the abandonment of animals near animal control agencies and the availability of food sources are the main hypotheses associated to the distribution of the captures. The awareness of the people who live in places where there is a greater number of stray animals and the distribution of the urban population are the main hypotheses to explain the distribution of the adoptions. The results will help to implement control measures over these populations in the most problematic areas.

  9. Pattern Classification in Kampo Medicine

    Directory of Open Access Journals (Sweden)

    S. Yakubo

    2014-01-01

    Full Text Available Pattern classification is very unique in traditional medicine. Kampo medical patterns have transformed over time during Japan’s history. In the 17th to 18th centuries, Japanese doctors advocated elimination of the Ming medical theory and followed the basic concepts put forth by Shang Han Lun and Jin Gui Yao Lue in the later Han dynasty (25–220 AD. The physician Todo Yoshimasu (1702–1773 emphasized that an appropriate treatment could be administered if a set of patterns could be identified. This principle is still referred to as “matching of pattern and formula” and is the basic concept underlying Kampo medicine today. In 1868, the Meiji restoration occurred, and the new government changed its policies to follow that of the European countries, adopting only Western medicine. Physicians trained in Western medicine played an important role in the revival of Kampo medicine, modernizing Kampo patterns to avoid confusion with Western biomedical terminology. In order to understand the Japanese version of traditional disorders and patterns, background information on the history of Kampo and its role in the current health care system in Japan is important. In this paper we overviewed the formation of Kampo patterns.

  10. Analyzing Technology Adoption - The Case of Kerala Home Gardens

    Directory of Open Access Journals (Sweden)

    Reeba Jacob

    2016-05-01

    Full Text Available Homegardens are traditional agroforestry system with a unique structure and function. It is the predominant farming system in Kerala. The study was undertaken in Thiruvananthapuram district covering a sample of 100 homegardens farmers from all the five agro-ecological units with an aim to assess the level of adoption of selected Kerala Agricultural University (KAU production practices in homegardens. Results of the study identified that majority of the farmers (63% belonged to medium level of adoption. Adoption quotient was worked out and compared with standard Rogers curve. Correlation analysis of the independent variables with the dependent variable viz., level of adoption indicated that age, farming experience, knowledge, evaluative perception, mass media contribution, livestock possession and extension contribution had direct significant effect on level of adoption of KAU production practices by homegarden farmers.

  11. Mediating Discourses of Transnational Adoption on the Internet

    DEFF Research Database (Denmark)

    McIlvenny, Paul; Raudaskoski, Pirkko Liisa

    2005-01-01

    on transnational adoption, and in those countries the adoption process is thoroughly regulated at all stages by the state. A few private agencies exist but they must be fully accredited -- for example, by the Danish Ministry of Justice in Denmark. Countries such as the USA have a much more liberalized ‘market......’ with less state regulation, so private sector agencies and lawyers flourish to facilitate parent-initiated adoptions (Dorow, 2002; Williams, 1994). The result is that quite different discourses are mobilized that are often more legalistic, individualistic and parent-centered. In both cases though...... of their experiences. Our goal in this paper is to document some of the ways in which mediational means are appropriated or contested in virtual spaces and the social consequences of these appropriations. Our case study focuses particularly on the discourses of adoption and the mediated actions of adoptive parents...

  12. ICT Adoption Policy of Australian and Croatian SMEs

    Directory of Open Access Journals (Sweden)

    Hazbo Skoko

    2006-03-01

    Full Text Available Many SMEs are currently adopting information and communication technology (ICT and services based on it. However, there is little systematic research into how they are doing this and what are the organisational and environmental factors associated with this adoption. In this article, the authors build the model of ICT adoption in Australian and Croatian SMEs, founded on premises that SMEs are the main economic developing factor in all modern economies and that the adoption and the use of ICT represents the fundamental source of competitiveness and the basis for their survival on the world market. By applying Qualitative Comparative Analysis (QCA and Boolean algebra, the authors developed a model of necessary and sufficient factors for ICT adoption by SMEs in Australia and Croatia.

  13. Risk of Suicide Attempt in Adopted and Nonadopted Offspring

    DEFF Research Database (Denmark)

    Keyes, M. A.; Malone, S. M.; Sharma, A.;

    2013-01-01

    OBJECTIVE: We asked whether adoption status represented a risk of suicide attempt for adopted and nonadopted offspring living in the United States. We also examined whether factors known to be associated with suicidal behavior would mediate the relationship between adoption status and suicide...... attempt. METHODS: Participants were drawn from the Sibling Interaction and Behavior Study, which included 692 adopted and 540 nonadopted offspring and was conducted at the University of Minnesota from 1998 to 2008. Adoptees were systematically ascertained from records of 3 large Minnesota adoption...... agencies; nonadoptees were ascertained from Minnesota birth records. Outcome measures were attempted suicide, reported by parent or offspring, and factors known to be associated with suicidal behavior including psychiatric disorder symptoms, personality traits, family environment, and academic...

  14. Exploring private consumers’ willingness to adopt Smart Grid technology

    DEFF Research Database (Denmark)

    Toft, Madeleine Broman; Thøgersen, John

    2015-01-01

    it involves behavioural changes. There is a need to get more insight into who are willing to adopt SGT and why. This study draws on innovation adoption theory as a framework for understanding consumer adoption of this new technology. We explore whether consumers who have already adopted other types of new......The goal of radically increasing the proportion of electricity generated from renewable sources puts the current electrical grid under pressure and one of the solutions is to turn the grid into a ‘Smart Grid’. One of the key elements of the Smart Grid is that electricity consumers make some...... of their consumption available as flexible capacity to balance the grid. Consumers’ flexible capacity is only available to the grid if the consumers adopt Smart Grid technology (SGT) that establishes the link between the electric system and the consumer. This technology is new to private consumers and using...

  15. Competitive Advantages Through it Innovation Adoption by Smes

    Directory of Open Access Journals (Sweden)

    S. Moghavvemi

    2012-07-01

    Full Text Available Purpose—This paper seeks to explain the impact of IT innovation on competitive advantage; the barriers and the benefits companies gain from adopting and using these innovations and propose a model with which it is possible to measure determinants of IT innovation adoption among SMEs.Design/methodology/approach—This paper presents conceptual consideration on the role of SMEs in the Malaysian economy and the effect of government policy in encouraging companies to adopt IT innovation. The proposed research framework will be empirically validated using survey data. This study is an ongoing research, in the existing stage a theoretical argument is developed and methodology is in the process of being tested through regression analysis.Findings—Conclusions are drawn on the status of Malaysian SMEs to adopt IT innovation. We added attitude and self-efficacy to the Innovation Diffusion Theory to suit it to the individual situation. Therefore a novel approach is needed in order to study and understand it.Research limitations/implications—The paper represents work in progress.Practical implications – This paper present the theoretical framework for further study of IT innovation adoption among Malaysia SMEs.Originality/Value—The Information Technology concept is considered a powerful competitive weapon in the modern economy. This study used Innovation Diffusion Theory as the base theory, and added attitude and self efficacy as determinants to measure the individual’s perception toward innovation adoption. Individual self-efficacy and attitude toward innovation adoption shape beliefs and perceptions toward innovation, leading them to adopt or reject an innovation. Adding these factors to Innovation Diffusion Theory will narrow the breadth of the theory and possibilities to frame a single study which allows examination of the individual and technological dimension toward technology adoption.Keywords: IT innovation adoption, competitive

  16. FACTORS INFLUENCING ADOPTION DECISIONS OF MAIZE FARMERS IN NIGERIA

    Directory of Open Access Journals (Sweden)

    Olusegun Ayodeji Fadare

    2014-07-01

    Full Text Available The needs to develop improved varieties of maize have been in the heart of various researchers and institutions in Nigeria because of its strategic role in tackling food insecurity and poverty. Despite substantial efforts to improve maize varieties, the level of adoption of improved maize varieties (IMV in Nigeria in still very low. Although previous adoption studies have indicated a number of socioeconomic and institutional/organizational variables as important factors influencing adoption of improved maize (crop varieties in the country, whether these factors are the main issues of concern, and whether the inclusion of regional/agro-ecological variables in adoption model are also important in explaining what could drive farmers’ adoption behaviour requires investigation. This study therefore examined factors influencing adoption of IMV among farmers in Nigeria using a selected portion of the Nigeria Living Standard Measurement Survey data collected by the National Bureau of Statistics and the World Bank for 2010/2011 cropping season with descriptive statistics and probit model as tools for data analysis. The results suggest, in line with some previous studies, that farm size, education level of farmers and access to extension services would significantly influence adoption of IMV. The results also indicate that farmers across the entire agro-ecological regions of country share some negative sentiments regarding adoption of IMV. Renewed emphasis on interventions that would enable farmers gain more access to farmland, and promote formal education and extension service are advocated. An attempt to incorporate variables that capture farmers’ perception/experience on agroclimatic/ ecologically related concerns in adoption study could aid better understand of what drives farmers’ adoption decisions across the country especially in the light of the emerging climate change issues and its implication on food production.

  17. Competitive Advantages Through it Innovation Adoption by Smes

    Directory of Open Access Journals (Sweden)

    S. Moghavvemi

    2013-01-01

    Full Text Available Purpose—This paper seeks to explain the impact of IT innovation on competitive advantage; the barriers and the benefits companies gain from adopting and using these innovations and propose a model with which it is possible to measure determinants of IT innovation adoption among SMEs. Design/methodology/approach—This paper presents conceptual consideration on the role of SMEs in the Malaysian economy and the effect of government policy in encouraging companies to adopt IT innovation. The proposed research framework will be empirically validated using survey data. This study is an ongoing research, in the existing stage a theoretical argument is developed and methodology is in the process of being tested through regression analysis. Findings—Conclusions are drawn on the status of Malaysian SMEs to adopt IT innovation. We added attitude and self-efficacy to the Innovation Diffusion Theory to suit it to the individual situation. Therefore a novel approach is needed in order to study and understand it. Research limitations/implications—The paper represents work in progress. Practical implications – This paper present the theoretical framework for further study of IT innovation adoption among Malaysia SMEs. Originality/Value—The Information Technology concept is considered a powerful competitive weapon in the modern economy. This study used Innovation Diffusion Theory as the base theory, and added attitude and self efficacy as determinants to measure the individual’s perception toward innovation adoption. Individual self-efficacy and attitude toward innovation adoption shape beliefs and perceptions toward innovation, leading them to adopt or reject an innovation. Adding these factors to Innovation Diffusion Theory will narrow the breadth of the theory and possibilities to frame a single study which allows examination of the individual and technological dimension toward technology adoption. Keywords: IT innovation adoption, competitive

  18. Proof patterns

    CERN Document Server

    Joshi, Mark

    2015-01-01

    This innovative textbook introduces a new pattern-based approach to learning proof methods in the mathematical sciences. Readers will discover techniques that will enable them to learn new proofs across different areas of pure mathematics with ease. The patterns in proofs from diverse fields such as algebra, analysis, topology and number theory are explored. Specific topics examined include game theory, combinatorics, and Euclidean geometry, enabling a broad familiarity. The author, an experienced lecturer and researcher renowned for his innovative view and intuitive style, illuminates a wide range of techniques and examples from duplicating the cube to triangulating polygons to the infinitude of primes to the fundamental theorem of algebra. Intended as a companion for undergraduate students, this text is an essential addition to every aspiring mathematician’s toolkit.

  19. What Constitutes Adoption of the Web: A Methodological Problem in Assessing Adoption of the World Wide Web for Electronic Commerce.

    Science.gov (United States)

    White, Marilyn Domas; Abels, Eileen G.; Gordon-Murnane, Laura

    1998-01-01

    Reports on methodological developments in a project to assess the adoption of the Web by publishers of business information for electronic commerce. Describes the approach used on a sample of 20 business publishers to identify five clusters of publishers ranging from traditionalist to innovator. Distinguishes between adopters and nonadopters of…

  20. Barriers and Strategies on Adoption of E-Learning in Tanzanian Higher Learning Institutions: Lessons for Adopters

    Science.gov (United States)

    Kisanga, Dalton; Ireson, Gren

    2015-01-01

    Tanzanian Higher learning institutions (HLIs) are faced with challenges of adopting e-learning in education. This study involved experts in e-learning to examine barriers of adopting e-learning and the best strategies to address them. Data were gathered from a series of semi-structured interviews with e-learning experts from two HLIs in Tanzania.…

  1. Pattern classification

    CERN Document Server

    Duda, Richard O; Stork, David G

    2001-01-01

    The first edition, published in 1973, has become a classic reference in the field. Now with the second edition, readers will find information on key new topics such as neural networks and statistical pattern recognition, the theory of machine learning, and the theory of invariances. Also included are worked examples, comparisons between different methods, extensive graphics, expanded exercises and computer project topics. An Instructor's Manual presenting detailed solutions to all the problems in the book is available from the Wiley editorial department.

  2. Same-Sex Adoption as a Welfare Alternative? Conservatism, Neoliberal Values, and Support for Adoption by Same-Sex Couples.

    Science.gov (United States)

    Perry, Samuel L; Whitehead, Andrew L

    2015-01-01

    Despite conservatives' long-term opposition to gay and lesbian parenting, scholars theorize that a strong commitment to neoliberalism may influence conservative Americans to become more tolerant of same-sex adoption as a way to relieve the government from subsidizing poor families. Drawing on national survey data (2010 Baylor Religion Survey), we test whether holding neoliberal values is associated with greater support for same-sex adoption in general and across political or religious conservatives. We find no support for either theory-emphatically the opposite, in fact. Neoliberal values are negatively associated with support for same-sex adoption for Americans in general and among political and religious conservatives. We find little evidence of a tension among conservatives regarding same-sex adoption as both their neoliberal values and moral beliefs incline them to oppose same-sex adoption along with other same-sex family relationships.

  3. East Meets West: Adopted Chinese Girls' Nighttime Sleep Problems and Adoptive Parents' Self-Judgment About Parenting.

    Science.gov (United States)

    Tan, Tony; Mahoney, Emily; Jackson, Andrea; Rice, Jessica

    2017-01-01

    We investigated the association between adopted Chinese girls' nighttime sleep problems and adoptive parents' self-judgment about their parenting. The girls were 1.7-6.9 years old (M = 4.6 years, SD = 1.0) and were adopted at 7-56 months (M = 13.9 months, SD = 6.6) by families in North America. At Wave 2 of a longitudinal study on adopted Chinese children's development, the adoptive parents provided survey data on bedtime resistance or anxiety and parasomnias in their daughters and their own parental sense of entitlement and parenting competence. Results showed that controlling for child and family demographics, parasomnias, but not bedtime resistance or anxiety, negatively predicted parental sense of entitlement (B = -.13, p parenting competence (B = -.14, p < .01).

  4. Factors Affecting Adoption of Recommended Cauliflower Production Technology in Nepal

    Directory of Open Access Journals (Sweden)

    Pankaj Raj Dhital

    2016-06-01

    Full Text Available Binary logit regression model of econometrics was used to identify the factors affecting adoption of recommended agricultural technology by the commercial farmers of Nepal. A survey was carried out in 2012 in 120 households from Kavre district, Nepal. The objective was to appraise factors affecting adoption of recommended technology of cauliflower, finding out the level of adoption, identifying the constraints of cauliflower cultivation, assessing the perceived level of satisfaction and studying the relationship of certain selected variables on the adoption. Education, Occupation, contact with Personal Localite sources of information, Group membership and Experience were the most influencing factors for adoption of recommended technology. Though, other factors were not strong enough to contribute significantly but indirectly influence the adoption decision of farmers as combined effects. Occupation was found as negatively associated. Landholding size was strongly and positively influencing among the non significant factors. The level of satisfaction and the constraints of cauliflower production were also identified. Transfer of technology will be effective if and only if the client adopts the technology. For an effective transfer of technology, it is better to go for activities for the welfare of the socioeconomic factors of the farmers.

  5. ADOPTION OF SMOKING CHOKOR FOR FISH PROCESSING IN KWARA STATE

    Directory of Open Access Journals (Sweden)

    J.A. Akangbe

    2013-03-01

    Full Text Available This research investigated the adoption of smoking chokor among fish processors in Kwara state, Nigeria. The study made use of structured questionnaire along with interview schedule to a sample size of one hundred and seventy (170 processors randomly selected through a two-stage random sampling technique. The data were analyzed with descriptive and inferential statistics and the Regression Analysis. The results obtained through the analysis revealed that 43.7% of the respondents adopted the improved technology (smoking chokor. A higher percentage of respondents preferred the improved technology for the golden brown colour, fine texture, good smell and delicacy in the taste. The significant determinants of adoption include sex (female, income from fish processing activities (p<0.05 and the trust worthiness of the source of the technology (p<0.1. Perceived benefits associated with technology adoption include wastage reduction, employment generation and the reduction of smoke, while the technicalities associated with the use constitute a major constraint limiting its adoption. The study concludes that fish processors prefer the smoking chokor but its adoption is marred with various constraints. It therefore recommends the need for creating more awareness of the smoking chokor as well as reducing the perceived constraints limiting its adoption by training extension agents and other verifiable sources to educate farmers as they disseminate the technology.

  6. Critical Factors for Personal Cloud Storage Adoption in China

    Directory of Open Access Journals (Sweden)

    Jianya Wang

    2016-06-01

    Full Text Available Purpose: In order to explain and predict the adoption of personal cloud storage, this study explores the critical factors involved in the adoption of personal cloud storage and empirically validates their relationships to a user's intentions. Design/methodology/approach: Based on technology acceptance model (TAM, network externality, trust, and an interview survey, this study proposes a personal cloud storage adoption model. We conducted an empirical analysis by structural equation modeling based on survey data obtained with a questionnaire. Findings: Among the adoption factors we identified, network externality has the salient influence on a user's adoption intention, followed by perceived usefulness, individual innovation, perceived trust, perceived ease of use, and subjective norms. Cloud storage characteristics are the most important indirect factors, followed by awareness to personal cloud storage and perceived risk. However, although perceived risk is regarded as an important factor by other cloud computing researchers, we found that it has no significant influence. Also, subjective norms have no significant influence on perceived usefulness. This indicates that users are rational when they choose whether to adopt personal cloud storage. Research limitations: This study ignores time and cost factors that might affect a user's intention to adopt personal cloud storage. Practical implications: Our findings might be helpful in designing and developing personal cloud storage products, and helpful to regulators crafting policies. Originality/value: This study is one of the first research efforts that discuss Chinese users' personal cloud storage adoption, which should help to further the understanding of personal cloud adoption behavior among Chinese users.

  7. A longitudinal investigation of children internationally adopted at school age.

    Science.gov (United States)

    Helder, Emily J; Mulder, Elizabeth; Gunnoe, Marjorie Linder

    2016-01-01

    Most existing research on children adopted internationally has focused on those adopted as infants and toddlers. The current study longitudinally tracked several outcomes, including cognitive, behavioral, emotional, attachment, and family functioning, in 25 children who had been internationally adopted at school age (M = 7.7 years old at adoption, SD = 3.4, range = 4–15 years). We examined the incidence of clinically significant impairments, significant change in outcomes over the three study points, and variables that predicted outcomes over time. Clinically significant impairments in sustained attention, full-scale intelligence, reading, language, executive functioning, externalizing problems, and parenting stress were common, with language and executive functioning impairments present at higher levels in the current study compared with past research focusing on children adopted as infants and toddlers. Over the three study points, significant improvements across most cognitive areas and attachment functioning were observed, though significant worsening in executive functioning and internalizing problems was present. Adoptive family-specific variables, such as greater maternal education, smaller family size, a parenting approach that encouraged age-expected behaviors, home schooling, and being the sole adopted child in the family were associated with greater improvement across several cognitive outcomes. In contrast, decreased parenting stress was predicted by having multiple adopted children and smaller family sizes were associated with greater difficulties with executive functioning. Child-specific variables were also linked to outcomes, with girls displaying worse attachment and poorer cognitive performance and with less time in orphanage care resulting in greater adoption success. Implications for future research and clinical applications are discussed.

  8. A Study of E-services Adoption Factors

    DEFF Research Database (Denmark)

    Scupola, Ada; Nicolajsen, Hanne Westh

    2011-01-01

    In this chapter we combine two subjects that are in our heart: e-services and diffusion of innovation. By drawing on earlier research on e-services, innovation, and technology adoption we investigate the factors that influence adoption of e-services in a specific academic library, Roskilde...... University Library (RUB). The conclusion of this research is that both external environmental factors and internal organizational factors are important factors in adoption of e-services at Roskilde University Library. However the study shows that external factors such as government intervention...

  9. Government Intervention in SMEs E-Commerce Adoption

    DEFF Research Database (Denmark)

    Hugger, Ada Scupola

    2005-01-01

    Institutional involvement, and especially the role of government, has historically been determinant in the adoption and diffusion of technological innovations. This study investigates the role of government in the adoption and diffusion of e-commerce in small and medium size enterprises. King et al....... (1994) framework of institutional factors in information technology innovation is used to analyze what is actually done and what SMEs would like to be done regarding government intervention to foster the adoption and diffusion of e-commerce. The findings show that the government could mostly influence...

  10. Exploring Leadership in E-commerce Adoption in Australian SMEs

    DEFF Research Database (Denmark)

    Scupola, Ada

    2010-01-01

    This chapter presents the results of a study investigating leadership and leadership styles in e-commerce adoption in small and medium size enterprises in Australia. The results show that top management and CEO' leadership have a key role in small and medium size enterprises (SMEs) in developing...... a vision for e-commerce adoption and that the dominant leadership style is directive with some signs of consultative. Furthermore the study shows that e-commerce adoption is becoming a strategic process and in this process top management is taking into consideration both the organizational knowledge...

  11. Cultural socialization in families with internationally adopted children.

    Science.gov (United States)

    Lee, Richard M; Grotevant, Harold D; Hellerstedt, Wendy L; Gunnar, Megan R

    2006-12-01

    Cultural socialization attitudes, beliefs, and parenting behaviors were examined in families with internationally adopted children. The authors hypothesized that parents with lower color-blind racial attitudes would be more likely to engage in enculturation and racialization parenting behaviors because they hold stronger beliefs in the value and importance of cultural socialization. Using data from the Minnesota International Adoption Project, the results support this mediation model of cultural socialization. Individual variations in cultural socialization also are discussed in terms of child development and shifting adoption attitudes and practices.

  12. A Conceptual Model of eLearning Adoption

    Directory of Open Access Journals (Sweden)

    Muneer Abbad

    2011-05-01

    Full Text Available Internet-based learning systems are being used in many universities and firms but their adoption requires a solid understanding of the user acceptance processes. The technology acceptance model (TAM has been used to test the acceptance of various technologies and software within an e-learning context. This research aims to discuss the main factors of a successful e-learning adoption by students. A conceptual research framework of e-learning adoption is proposed based on the TAM model.

  13. Adoptive T cell therapy: Addressing challenges in cancer immunotherapy

    Directory of Open Access Journals (Sweden)

    Yee Cassian

    2005-04-01

    Full Text Available Abstract Adoptive T cell therapy involves the ex vivo selection and expansion of effector cells for the treatment of patients with cancer. In this review, the advantages and limitations of using antigen-specific T cells are discussed in counterpoint to vaccine strategies. Although vaccination strategies represent more readily available reagents, adoptive T cell therapy provides highly selected T cells of defined phenotype, specificity and function that may influence their biological behavior in vivo. Adoptive T cell therapy offers not only translational opportunities but also a means to address fundamental issues in the evolving field of cancer immunotherapy.

  14. The drivers to adopt renewable energy among residential users.

    Science.gov (United States)

    Rahman, Zahari Abdul; Elinda, Esa

    2016-03-01

    This study aims to examine the drivers to adopt renewable energy (RE) among residential users in Malaysia. Based on the theoretical framework of a consumer’s decision making process, an empirical study of the adoption of RE was conducted. A total of 501 residential users were used in this study. This study proved that perceived utility of new technology, perceived utility of new service, and perceived benefit of new technology are the drivers to adopt RE among residential users. These factors are knowing crucial to RE suppliers and producers because it will generates more demand from the residential users and the percentage of energy mix from RE sources can be increase.

  15. Impact of Perceived Security on Organizational Adoption of Smartphones.

    Science.gov (United States)

    Arpaci, Ibrahim; Yardimci Cetin, Yasemin; Turetken, Ozgur

    2015-10-01

    Organizational adoption of new technologies is a practically important research area. The present study aims to investigate the impact of perceived security on organizational adoption of mobile communication technologies, specifically smartphones. For this purpose, a research model is developed extending the Technology Acceptance Model (TAM). The research model is tested using a structural equation modeling based on data that were collected from senior managers of 201 private sector organizations in Turkey. The results show that, along with the traditional constructs of perceived ease of use and perceived usefulness, perceived security significantly affects organizations' adoption of smartphones. Both the theoretical and practical implications of these results are discussed.

  16. Innovators and Early Adopters of Distance Education in Social Work

    OpenAIRE

    Jo Ann Coe Regan

    2016-01-01

    This article highlights the innovators and early adopters of distance education in social work. The past, present and future is discussed as it relates to the evolution of technology innovation in social work education.

  17. Predicting Public Bicycle Adoption Using the Technology Acceptance Model

    Directory of Open Access Journals (Sweden)

    Benjamin T. Hazen

    2015-10-01

    Full Text Available Bicycle sharing programs provide a sustainable mode of urban transportation. Although cities across the globe have developed these systems for their citizens and visitors, usage rates are not as high as anticipated. This research uses the technology acceptance model as the basis to understand one’s intention to adopt bicycle sharing programs. Using survey data derived from 421 participants in Beijing, China, the proposed covariance-based structural equation model consisting of perceived quality, perceived convenience, and perceived value is found to predict 50.5% of the variance in adoption intention. The findings of this research contribute to theory and practice in the burgeoning literature on public bicycle systems and sustainable urban transportation by offering a theoretical lens through which to consider system adoption, and providing information to practitioners as to what factors might contribute most to adoption.

  18. Six sigma and lean production adoption in a manufacturing company

    Directory of Open Access Journals (Sweden)

    Alisson Christian Scheller

    2014-11-01

    Full Text Available The connection among Lean Production with Six Sigma originated the Lean Six Sigma methodology, focused on processes variation and waste reduction. This methodology was developed on different ways in the companies and there is no consensus over its structure and its implementation. In this context, this paper aims to identify and analyze the main characteristics on the adoption and integration of Lean Six Sigma methodology through a case study conducted in a manufacturing company that adopts lean production and six sigma. The results show two important aspects of the Lean Six Sigma methodology. One of them is the adoption of the value stream mapping as a central tool on Lean Six Sigma. The other is the use of DMAIC for improvements actions. The study indicates that despite the difficulties on Lean Six Sigma implementation, the methodology offers benefits to the company that adopts it in the suitable way.

  19. Adoption of information security measures in public research institutes

    Directory of Open Access Journals (Sweden)

    Antonio Eduardo de Albuquerque Junior

    2015-10-01

    Full Text Available There are several Information Security measures recommended by international standards and literature, but the adoption by the organizations should be buoyed by specific needs identified by Information Security Governance structure of each organization, although it may be influenced by forces of the institutional environment in which organizations are inserted. In public research institutes, measures may be adopted as a result of pressure from Government and other organizations that regulate their activities, or by the influence of Information Security professionals, or simply adopting the same measures of leading organizations in the organizational field. This study aimed to investigate whether in public research institutes the adoption of Information Security measures is influenced by organizational factors relating to the Information Security Governance, and by external factors relating to its institutional environment. The results show that these organizations are subject to institutional influences more than organizational influences.

  20. Adopting Communicative Language Teaching in English Classes in China

    Institute of Scientific and Technical Information of China (English)

    沈轶

    2015-01-01

    Communicative Language Teaching ( CLT) can promote learners’ communicative competence in the target language, and it can definitely contribute to the English education reform in China. However, some problems of adopting CLT can not be ignored.

  1. Marketing Decision Support Systems: Adoption, Use and Satisfaction

    NARCIS (Netherlands)

    B. Wierenga (Berend); P.A.M. Oude Ophuis

    1997-01-01

    textabstractThis paper deals with marketing decision support systems (MDSS) in companies. In a conceptual framework five categories of factors are distinguished that potentially affect adoption, use, and satisfaction: external environment factors, organizational factors, task environment factors, us

  2. Marketing decision support systems: Adoption, use and satisfaction.

    NARCIS (Netherlands)

    Wierenga, B.; Oude Ophuis, P.A.M.

    1997-01-01

    This paper deals with marketing decision support systems (MDSS) in companies. In a conceptual framework five categories of factors are distinguished that potentially affect adoption, use, and satisfaction: external environment factors, organizational factors, task environment factors, user factors a

  3. Which retailers adopt a loyalty program? An empirical study

    NARCIS (Netherlands)

    Leenheer, J.; Bijmolt, T.H.A.

    2008-01-01

    This paper examines antecedents of retailers’ loyalty program adoption and their perceptions regarding loyalty program effectiveness. The investigated antecedents consists of sector, competitive and demand, and firm characteristics. To test the hypotheses, we surveyed 180 retail companies active in

  4. An unexpected reversal: the "demise" of international adoption in Brazil

    Directory of Open Access Journals (Sweden)

    Claudia Fonseca

    2007-01-01

    Full Text Available During the 90s, there was a dramatic fall in intercountry adoptions of Brazilian children - from over 2000 per annum at the beginning of the decade, to under 400 at decade's close. In this paper, on the basis of archival material, we will attempt to outline possible reasons for this drop, considering hypotheses linked to the international market of adoptable children, legal restrictions on intercountry adoption imposed by the Brazilian government, and, finally, scandals in the mass media which stigmatize local intermediaries and officials involved in this activity. We conclude that inflamed public opinion and scandals in the media, although highly influential, are not necessarily the most adequate ingredients for a sensible policy on adoption.

  5. Influence of Culture on the Process of Managing Decisions Adoption

    Directory of Open Access Journals (Sweden)

    Florin-Lucian Isac

    2015-01-01

    Full Text Available Different cultural environment requires a corresponding managerial environment. The process of managing decisions adoption is influenced by the values, attitudes, beliefs and behaviors of the employees.

  6. Innovation adoption processes for third party property management companies

    Energy Technology Data Exchange (ETDEWEB)

    Shockman, Chris; Piette, Mary Ann

    2000-07-01

    Innovation adoption studies have never been applied to third party property management companies. These companies manage buildings for a fee as their primary business. Property management companies are influential in the adoption process for new technologies because they act as gatekeepers for technical information. This study analyzes radical and routine adoption process that are found in large, professionally operated property management companies. The process is explicated. The technical managers, and their role as technology gate keepers, are described. The distinction to the technical managers between routine and radical technology is that routine technologies do something in a new way and radical technologies do something new. Observations concerning evaluation and adoption of information technologies are described. The findings suggest methods of successfully tailoring and introducing technologies to this market.

  7. 17 CFR 200.67 - Power to adopt rules.

    Science.gov (United States)

    2010-04-01

    ... AND ETHICS; AND INFORMATION AND REQUESTS Canons of Ethics § 200.67 Power to adopt rules. In exercising... rules should never tend to stifle or discourage legitimate business enterprises or activities,...

  8. Pregnancy Choices: Raising the Baby, Adoption, and Abortion

    Science.gov (United States)

    ... PREGNANCY Pregnancy Choices: Raising the Baby, Adoption, and Abortion • What are my options if I find out ... is financial help available? • If I am considering abortion, what should I know about my state’s laws? • ...

  9. Exploring Factors Influencing the Adoption of Mobile Commerce

    Directory of Open Access Journals (Sweden)

    Tariq Bhatti,

    2007-12-01

    Full Text Available This study presents an extended technology acceptance model that integrates innovation diffusion theory to investigate what determine user mobile commerce acceptance. This paper models the factors relationships such as perceived usefulness, perceived ease of use, personal innovativene ss, subjective norms, behavioral control and intention to adopt mobile commerce. The proposed model was empirically tested using data collected from a survey of mobile commerce consumers. Empirical data from regression analysis reflects users ease of use influence behavioral intention to adopt mobile commerce. The majority of positive relationships between perceived ease of use, subjective norms, behavioral control and intention to adopt are supported by empirical data. Results also reveal that behavioral control and subjective norms influence perceived ease of use which affects then their adoption intention. The paper concludes some important implications for the practitioners.

  10. Standard Systems Group (SSG) Technology Adoption Planning Workshop

    Science.gov (United States)

    2004-04-01

    Internet Routed Protocol Network (SIPRNET) architecture • Replace the current 5-year old enterprise network backup system • Improve of current IPTV ...Standard Systems Group (SSG) Technology Adoption Planning Workshop Suzanne Garcia Lorraine Nemeth-Adams Jan Vargas April 2004...Standard Systems Group(SSG) Technology Adoption Planning Workshop 5a. CONTRACT NUMBER 5b. GRANT NUMBER 5c. PROGRAM ELEMENT NUMBER 6. AUTHOR(S) 5d

  11. Factors affecting adoption of mobile banking in Pakistan: Empirical Evidence

    Directory of Open Access Journals (Sweden)

    Abdul Kabeer Kazi

    2016-01-01

    Hyderabad of the province Sindh, in Pakistan using judgement sampling method. This study empirically concluded that consumers’ intention to adopt mobile banking services was significantly influenced by social influence, perceived risk, perceived usefulness, and perceived ease of use. The most significant positive impact was of social influence on consumers’ intention to adopt mobile banking services. The paper concluded with discussion on results, and several business implications for the banking industry of Pakistan.

  12. Cultural Socialization in Families With Internationally Adopted Children

    OpenAIRE

    Lee, Richard M.; Grotevant, Harold D.; Hellerstedt, Wendy L.; Gunnar, Megan R.

    2006-01-01

    Cultural socialization attitudes, beliefs, and parenting behaviors were examined in families with internationally adopted children. The authors hypothesized that parents with lower color-blind racial attitudes would be more likely to engage in enculturation and racialization parenting behaviors because they hold stronger beliefs in the value and importance of cultural socialization. Using data from the Minnesota International Adoption Project, the results support this mediation model of cultu...

  13. Health Information Technology Adoption in California Community Health Centers

    Science.gov (United States)

    Kim, Katherine K.; Rudin, Robert S.; Wilson, Machelle D.

    2016-01-01

    Objectives National and state initiatives to spur adoption of electronic health record (EHR) use and health information exchange (HIE) among providers in rural and underserved communities have been in place for 15 years. Our goal was to systematically assess the impact of these initiatives by quantifying the level of adoption and key factors associated with adoption among community health centers (CHCs) in California. Study Design Cross-sectional statewide survey. Methods We conducted a telephone survey of all California primary care CHCs from August to September 2013. Multiple logistic regressions were fit to test for associations between various practice characteristics and adoption of EHRs, Meaningful Use (MU)–certified EHRs, and HIE. For the multivariable model, we included those variables which were significant at the P = .10 level in the univariate tests. Results We received responses from 194 CHCs (73.5% response rate). Adoption of any EHRs (80.3%) and MU–certified EHRs (94.6% of those with an EHR) was very high. Adoption of HIE is substantial (48.7%) and took place within a few years (mean = 2.61 years; SD = 2.01). More than half (54.7%) of CHCs are able to receive data into the EHR, indicating some level of interoperability. Patient engagement capacity is moderate, with 21.6% offering a personal health record, and 55.2% electronic visit summaries. Rural location and belonging to a multi-site clinic organization both increase the odds of adoption of EHRs, HIE, and electronic visit summary, with odds ratios ranging from 0.63 to 3.28 (all P values <.05). Conclusions Greater adoption of health information technology (IT) in rural areas may be the result of both federal and state investments. As CHCs lack access to capital for investments, continued support of technology infrastructure may be needed for them to further leverage health IT to improve healthcare. PMID:26760431

  14. What motivates European firms to adopt environmental management systems?

    OpenAIRE

    Rivera Camino, Jaime

    2001-01-01

    The growing world concern about the impact of industrial activities on the environment has motivated companies to adopt Environmental Management Systems (EMSs). Despite this trend, very Httle has been written about the variables associated with the implementation of this type of management tool. This article describes the individual, strategic and organizational variables that a sample of European firms use to determine whether to adopt these EMS. It also ...

  15. Demand Heterogeneity and the Adoption of Platform Complements

    OpenAIRE

    Rietveld, Joost; Eggers, J.P.

    2016-01-01

    textabstractThis paper offers a demand-based theory of how platform maturity affects the adoption of platform complements. We argue that differences between early and late adopters of the platform include willingness to pay for the platform-and-complement bundle, risk preferences, preference for novelty, and search behavior. These differences create heterogeneous demand conditions for complements that affect both average complement performance and variance in the types of complements that are...

  16. Adoption of Maize Conservation Tillage in Azuero, Panama

    OpenAIRE

    de Herrera, Adys Pereira; Sain, Gustavo

    1999-01-01

    An aggressive research and validation program launched in 1984 in Azuero, Panama, yielded a recommendation advocating zero tillage for maize production. Ten years later, maize farmers in Azuero used three land preparation methods: conventional tillage, zero tillage, and minimum tillage (an adaptation of the zero tillage technology). This study aimed to quantify the adoption of zero and minimum tillage for maize in Azuero; identify factors influencing adoption of the different land preparation...

  17. HACCP/RMP Adoption in the New Zealand Meat Industry

    OpenAIRE

    Cao, Kay; Scrimgeour, Frank G.

    2004-01-01

    In New Zealand, the Animal Products Act 1999 requires that all animal product primary processing businesses must have a risk management programme (RMP) based on the principles of Hazards Analysis and Critical Control Point (HACCP). However, due to market access requirements, many primary food exporters have voluntarily adopted HACCP systems for food safety management since the 1990s. This paper studies the process of HACCP/RMP adoption and the transition from voluntary HACCP to mandatory RMP ...

  18. A PRINCIPAL-AGENT MODEL FOR REGIONAL PEST CONTROL ADOPTION

    OpenAIRE

    Ahouissoussi, Nicolas B.C.

    1995-01-01

    Investigating the underlying producer characteristics associated with regional pest control adoption revealed an interesting proposition. Early adopting producers of firm-specific techniques with characteristics including higher education, more specialized operations, and larger sized business units are dissatisfied with a regional pest control technique. This study provides an explanation of the proposition based on a principal-agent model. Empirical support for the proposition is also prese...

  19. Are parents really attached to their adopted children?

    OpenAIRE

    Subhani, Muhammad Imtiaz; Osman, Amber; Abrar, Fariha; Hasan, Syed Akif

    2014-01-01

    Background Psychological studies found that adopted children suffer from lack of attachment relationships in life. It is important for new parents to understand the underlying concepts before they begin to comprehend behavior issues arising out of different turbulent situations in an adopted child’s life. Attachment theory facilitates in comprehending the frame of mind of these children, when they come from emotionally turbulent backgrounds and how some, if not all behavior issues can be atte...

  20. THE REVIEW OF INCENTIVE FACTORS FOR eBANKING ADOPTION

    OpenAIRE

    Liliana Moga; Radu Stroe; Mişu Nicoleta Bărbuţă

    2011-01-01

    This research is conducted to analyse the main factors which have emerged in classical theories related to intention to adopt the eBanking services by customers. The study is based on several models that have been created for describing and predicting the reasons for users accepting or rejecting a piece of technology. These models identified three factors that would influence the adoption of Internet banking: attitude, subjective norms and perceived behavioural control. Finally, we realised a...

  1. Local cascades induced global contagion: How heterogeneous thresholds, exogenous effects, and unconcerned behaviour govern online adoption spreading

    Science.gov (United States)

    Karsai, Márton; Iñiguez, Gerardo; Kikas, Riivo; Kaski, Kimmo; Kertész, János

    2016-06-01

    Adoption of innovations, products or online services is commonly interpreted as a spreading process driven to large extent by social influence and conditioned by the needs and capacities of individuals. To model this process one usually introduces behavioural threshold mechanisms, which can give rise to the evolution of global cascades if the system satisfies a set of conditions. However, these models do not address temporal aspects of the emerging cascades, which in real systems may evolve through various pathways ranging from slow to rapid patterns. Here we fill this gap through the analysis and modelling of product adoption in the world’s largest voice over internet service, the social network of Skype. We provide empirical evidence about the heterogeneous distribution of fractional behavioural thresholds, which appears to be independent of the degree of adopting egos. We show that the structure of real-world adoption clusters is radically different from previous theoretical expectations, since vulnerable adoptions—induced by a single adopting neighbour—appear to be important only locally, while spontaneous adopters arriving at a constant rate and the involvement of unconcerned individuals govern the global emergence of social spreading.

  2. EXPLORING THE FACTORS AFFECTING EMPLOYEES’ ADOPTION AND USE OF INNOVATION

    Directory of Open Access Journals (Sweden)

    Majharul Talukder

    2010-01-01

    Full Text Available This paper explores the factors affecting innovation adoption by individual employees within an organization in Australia. Following a qualitative research approach, this paper uses a series of in-depth interviews and focus group discussions involving academic and administrative employees at a tertiary educational institution in Australia. The qualitative approach is adopted to have a deeper insight into the complexities and dynamism associated with the factors influencing innovation adoption process in organizational settings. The findings of this research highlight the need for broadening an understanding of the key factors affecting employee’s decisions to uptake the adoption of innovation. The results provide important clues for comprehending the factors influencing and determining the employee’s adoption and continued use of innovation in the work environment. In the face of the current trend towards end-user applications of technological innovation, the results suggest some guidelines for management toward effective and efficient adoption and use of innovation in organizational settings. This paper has highlighted the above findings and their implications for management practices related to innovation management in an Australian organizational setting. This paper also identifies possible limitations and future research potential in the relevant field.

  3. Comparative analysis on the adoption of innovation in furniture companies

    Directory of Open Access Journals (Sweden)

    Rodolfo Reinaldo Hermes Petter

    2013-06-01

    Full Text Available This study aimed to identify factors categorical adoption of innovation in three main groups: in products, processes and administrative procedures in a group of micro and small enterprises in the furniture sector, located in the middle western state of Santa Catarina. In particular, the study sought to compare the data of a survey conducted by SEBRAE (2009 observed the same factors present innovation in micro and small businesses scattered throughout the Brazilian territory, in order to compare the national stage for the adoption of innovation in relation the scenario presented by the companies studied in this research. Data collection took place by means of a questionnaire consisting of multiple choice closed questions related to the three aforementioned groups of innovation, which was answered by managers of the constituent companies of the group studied. As a result of this research, it was found that the adoption of innovation in product development is the most important aspect in the surveyed companies. It was also found that they seek to adopt the innovation with the objective of entering new markets. However, the main difficulty in adopting innovation were identified lack of skilled labor, shortage and / or lack of access to technology and financial difficulties. Compared to general adoption of innovation by micro and small enterprises studied by SEBRAE (2009, the studied firms have a tendency to develop in the same direction.

  4. Rainwater harvesting: theorising and modelling issues that influence household adoption.

    Science.gov (United States)

    White, I

    2010-01-01

    Household adoption of rainwater harvesting (RH) systems recently boomed in Australian urban centres. As a sustainable supplement to the mains supply, water authorities regarded RH as a demand management device. Yet what actually motivated household RH adoption was not well understood. Burawoy's 'extended case method' was used to link grounded enquiry with 13 core theoretical concepts drawn from an original synthesis of ecological modernisation and diffusion of innovation theories. This framework means the household experience extends ideas drawn from existing theory to explore issues that influence the household RH adoption decision. The framework was interrogated with data drawn from a self-report survey of 560 homes. It accommodated 100% of the RH adoption issues identified by households. Further staged quantitative and qualitative analyses particularized these core concepts out to 36 discrete 'subfactors' which were used in discriminant function analyses. 17 subfactors contribute to a discriminant function grouping 89.2% of households to the correct 'adopter' or 'nonadopter' category (pRH adoption and can be applied to social marketing aimed at facilitating further penetration of household RH.

  5. Factors Influencing Organization Adoption Decision On Cloud Computing

    Directory of Open Access Journals (Sweden)

    Ailar Rahimli

    2013-01-01

    Full Text Available Cloud computing is a developing field, using by organization that require to computing resource to provide the organizational computing needs. The goal of this research is evaluate the factors that influence on organization decision to adopt the cloud computing in Malaysia. Factors that relate to cloud computing adoption that include : need for cloud computing, cost effectiveness, security effectiveness of cloud computing and reliability. This paper evaluated the factors that influence on adoption decision from view point of management decision making. The variable that evaluate in this study include: Cost effectiveness, Need for cloud computing, Security effectiveness, and reliability on cloud computing as independent variable and Adoption decision as dependent variable. The scope of this research is IT companies in Malaysia, and target population is member of organization that want to make decision to adopt the cloud computing. The Correlation and Multi Regression analysis show that all factor s have a positive and significant effect on organization adoption decision to cloud computing. 

  6. Accounting quality and the adoption of IASB standards: portuguese evidence

    Directory of Open Access Journals (Sweden)

    Ana Isabel Morais

    2008-12-01

    Full Text Available Through rule 1606/2002 by the European Commission, Portuguese listed firms were required to adopt IASB standards in the preparation and presentation of consolidated accounts for the periods beginning on or after 2005. IASB standards are developed in environments where accounting practices are especially directed at the private sector, reporting rules are largely unaffected by taxation requirements and capital is traditionally raised in public markets. However, in Portugal, financial reporting is closely related to tax reporting and banks play a key role in providing finance and inside access to information. We investigate whether adopting IASB standards is associated with higher earnings quality and higher value relevance. We compare the earnings quality and value relevance of accounting data of 34 Portuguese listed firms before (1995-2004 and after (2004-2005 the adoption of IASB standards. We find that firms, during the period when they adopt IASB standards, report less smooth earnings than those firms in periods when they adopted national accounting standards, which seems to suggest an improvement in earnings quality. However, we also find that the value relevance of accounting information decreases with the adoption of IASB standards.

  7. Parent-child communication and adolescent self-esteem in separated, intercountry adoptive and intact non-adoptive families.

    Science.gov (United States)

    Lanz, M; Iafrate, R; Rosnati, R; Scabini, E

    1999-12-01

    The purpose of the present study was to verify whether there are some differences in parent-child communication and in adolescent self-esteem among adoptive, separated and intact non-adoptive families and to investigate the extent to which parent-child communication is related to adolescent self-esteem in the three types of families. The study sample was composed of 450 adolescents aged between 11 and 17 years (160 from intact non-adoptive families, 140 from separated or divorced families and 150 from intercountry adoptive families). Subjects completed the Parent-Adolescent Communication Scale by Barnes and Olson, the Rosenberg Self-esteem Scale and some socio-demographic items. The results show that adolescents from separated families have more difficulties in their relationships with both the mother and the father than their peers, and that adoptive children perceive a more positive communication with their parents than biological children. Moreover, adoptees showed lower self-esteem than the other two groups of adolescents. Lastly, it emerged that male and female adolescents' self-esteem is related to positive communication with both parents in intact non-adoptive families, while no link was significant for male and female children of divorced parents or for adoptees.

  8. Health, agricultural, and economic effects of adoption of healthy diet recommendations.

    Science.gov (United States)

    Lock, Karen; Smith, Richard D; Dangour, Alan D; Keogh-Brown, Marcus; Pigatto, Gessuir; Hawkes, Corinna; Fisberg, Regina Mara; Chalabi, Zaid

    2010-11-13

    Transition to diets that are high in saturated fat and sugar has caused a global public health concern, as the pattern of food consumption is a major modifiable risk factor for chronic non-communicable diseases. Although agri-food systems are intimately associated with this transition, agriculture and health sectors are largely disconnected in their priorities, policy, and analysis, with neither side considering the complex inter-relation between agri-trade, patterns of food consumption, health, and development. We show the importance of connection of these perspectives through estimation of the eff ect of adopting a healthy diet on population health, agricultural production, trade, the economy, and livelihoods,with a computable general equilibrium approach. On the basis of case-studies from the UK and Brazil, we suggest that benefits of a healthy diet policy will vary substantially between different populations, not only because of population dietary intake but also because of agricultural production, trade, and other economic factors.

  9. The Minnesota Adoption Studies: genetic differences and malleability.

    Science.gov (United States)

    Scarr, S; Weinberg, R A

    1983-04-01

    In 1974 we launched 2 large adoption studies for 2 quite different purposes. The Transracial Adoption Study was designed to test the hypothesis that black and interracial children reared by white families perform on IQ and school achievement tests as well as other adoptees because they are reared in the culture of the tests and the schools. In addition, transracial families provided a sample with large numbers of adopted and natural children in the same families. Sources of individual differences among siblings could be studied without fear of possible differences between adoptive families and those with their own children. The Adolescent Adoption Study was designed to assess the cumulative impact of differences among family environments at the end of the child-rearing period. All of the children were adopted in the first year of life and averaged 18.5 years at the time of the study. A comparison sample of families with their own adolescents was also studied. Black and interracial children scored as well on IQ tests as adoptees in other studies. Individual differences among them, however, were more related to differences among their biological than adoptive parents, whether they lived together or not. Young siblings were found to be intellectually quite similar, whether genetically related or not. Adolescents' IQ test scores were similar to those of their parents and siblings only if they were biologically related. Our interpretation of these results is that younger children are more influenced by differences among their family environments than older adolescents, who are freer to seek their own niches.

  10. Dairy technology adoption in smallholder farms in "Dejen" district, Ethiopia.

    Science.gov (United States)

    Mekonnen, H; Dehninet, G; Kelay, B

    2010-02-01

    Factors influencing dairy technology adoption and impact on milk yield were studied in 240 smallholder farms in Dejen district, Ethiopia. The majority of the smallholders had more than 20 years of farming experience and were living at more than 10 km distance from market or trading centers (67% and 54% in that order). The dairy technologies studied included the use of crossbred animals, improved feed technologies and improved management practices. Application of acaricides, deworming, vaccination, heat-detection and haymaking had wide application (more than 80% adoption levels) while urea straw treatment, silage making, rotational grazing and fodder beet development were the least practiced ones. Only 20 percent of the cows were crossbred animals. It has been found that higher level of technology adoption is associated with better milk yield regardless of the breed of cattle (local or crossbred) owned by the farmers. Milk yields in local breeds increased by 0.07 times when the number of technologies increased by one unit. In crossbred cows, this rate of increase was five fold higher (0.38 times for one unit increase). Correlation coefficients between and within farm household characteristics and technologies adoption were, generally, significant. Male-headed households adopted significantly higher number of technologies than female-headed households (P < 0.001). Technology adoption rates increased significantly with increased education level and family size and decreasing distance from market or trade centers (P < 0.01). The level of technology adoption by smallholder farmers is still unsatisfactory and is highly dependent on gender, family size and level of education of smallholder farmers and location of farms.

  11. Why, when, and how fast innovations are adopted

    Science.gov (United States)

    Gonçalves, S.; Laguna, M. F.; Iglesias, J. R.

    2012-06-01

    When the full stock of a new product is quickly sold in a few days or weeks, one has the impression that new technologies develop and conquer the market in a very easy way. This may be true for some new technologies, for example the cell phone, but not for others, like the blue-ray. Novelty, usefulness, advertising, price, and fashion are the driving forces behind the adoption of a new product. But, what are the key factors that lead to adopt a new technology? In this paper we propose and investigate a simple model for the adoption of an innovation which depends mainly on three elements: the appeal of the novelty, the inertia or resistance to adopt it, and the interaction with other agents. Social interactions are taken into account in two ways: by imitation and by differentiation, i.e., some agents will be inclined to adopt an innovation if many people do the same, but other will act in the opposite direction, trying to differentiate from the "herd". We determine the conditions for a successful implantation of the new technology, by considering the strength of advertising and the effect of social interactions. We find a balance between the advertising and the number of anti-herding agents that may block the adoption of a new product. We also compare the effect of social interactions, when agents take into account the behavior of the whole society or just a part of it. In a nutshell, the present model reproduces qualitatively the available data on adoption of innovation.

  12. Research misconduct definitions adopted by U.S. research institutions.

    Science.gov (United States)

    Resnik, David B; Neal, Talicia; Raymond, Austin; Kissling, Grace E

    2015-01-01

    In 2000, the U.S. federal government adopted a uniform definition of research misconduct as fabrication, falsification, or plagiarism (FFP), which became effective in 2001. Institutions must apply this definition of misconduct to federally-funded research to receive funding. While institutions are free to adopt definitions of misconduct that go beyond the federal standard, it is not known how many do. We analyzed misconduct policies from 183 U.S. research institutions and coded them according to thirteen different types of behavior mentioned in the misconduct definition. We also obtained data on the institution's total research funding and public vs. private status, and the year it adopted the definition. We found that more than half (59%) of the institutions in our sample had misconduct policies that went beyond the federal standard. Other than FFP, the most common behaviors included in definitions were "other serious deviations" (45.4%), "significant or material violations of regulations" (23.0%), "misuse of confidential information" (15.8%), "misconduct related to misconduct" (14.8%), "unethical authorship other than plagiarism" (14.2%), "other deception involving data manipulation" (13.1%), and "misappropriation of property/theft" (10.4%). Significantly more definitions adopted in 2001 or later went beyond the federal standard than those adopted before 2001 (73.2% vs. 26.8%), and significantly more definitions adopted by institutions in the lower quartile of total research funding went beyond the federal standard than those adopted by institutions in the upper quartiles. Public vs. private status was not significantly associated with going beyond the federal standard.

  13. Critical analysis of key determinants and barriers to digital innovation adoption among architectural organizations

    Directory of Open Access Journals (Sweden)

    Runddy Ramilo

    2014-12-01

    Full Text Available The development and use of design technology for architecture in the modern world have led to the emergence of various design methodologies. Current design research has focused on a computationally mediated design process. This method is essentially concerned with finding forms and building performance simulation, i.e., structural, environmental, constructional, and cost performance, by integrating physics and algorithms. From the emergence of this process, design practices have been increasingly aided by and dependent on the technology, which has resulted in a major paradigm shift. Advancement of the new technology has the potential to improve design and productivity dramatically. However, related literature shows that substantial technical and organizational barriers exist. These barriers inhibit the effective adoption of these technologies. The effect of these obstacles on architectural practice varies depending on the size of an architectural organization. To further understand the problem, we conducted an in-depth study on several small, medium, and large architectural organizations. This study involves in-depth evaluation of technological, financial, organizational, governmental, psychological, and process barriers encountered in the adoption of digital innovation. Results reveal relevant attributes and patterns of variables, which can be used to establish a framework for digital innovation adoption. Valuable findings of this study reveal that smaller architectural organizations present more barriers to digital innovation compared with their larger counterparts. This study is important because it contributes to the research on digital innovation in architecture and addresses the barriers faced by different sizes of architectural organizations.

  14. Towards a Model of Technology Adoption: A Conceptual Model Proposition

    Science.gov (United States)

    Costello, Pat; Moreton, Rob

    A conceptual model for Information Communication Technology (ICT) adoption by Small Medium Enterprises (SMEs) is proposed. The research uses several ICT adoption models as its basis with theoretical underpinning provided by the Diffusion of Innovation theory and the Technology Acceptance Model (TAM). Taking an exploratory research approach the model was investigated amongst 200 SMEs whose core business is ICT. Evidence from this study demonstrates that these SMEs face the same issues as all other industry sectors. This work points out weaknesses in SMEs environments regarding ICT adoption and suggests what they may need to do to increase the success rate of any proposed adoption. The methodology for development of the framework is described and recommendations made for improved Government-led ICT adoption initiatives. Application of the general methodology has resulted in new opportunities to embed the ethos and culture surrounding the issues into the framework of new projects developed as a result of Government intervention. A conceptual model is proposed that may lead to a deeper understanding of the issues under consideration.

  15. Agent-based modelling of heating system adoption in Norway

    Energy Technology Data Exchange (ETDEWEB)

    Sopha, Bertha Maya; Kloeckner, Christian A.; Hertwich, Edgar G.

    2010-07-01

    Full text: This paper introduces agent-based modelling as a methodological approach to understand the effect of decision making mechanism on the adoption of heating systems in Norway. The model is used as an experimental/learning tool to design possible interventions, not for prediction. The intended users of the model are therefore policy designers. Primary heating system adoptions of electric heating, heat pump and wood pellet heating were selected. Random topology was chosen to represent social network among households. Agents were households with certain location, number of peers, current adopted heating system, employed decision strategy, and degree of social influence in decision making. The overall framework of decision-making integrated theories from different disciplines; customer behavior theory, behavioral economics, theory of planned behavior, and diffusion of innovation, in order to capture possible decision making processes in households. A mail survey of 270 Norwegian households conducted in 2008 was designed specifically for acquiring data for the simulation. The model represents real geographic area of households and simulates the overall fraction of adopted heating system under study. The model was calibrated with historical data from Statistics Norway (SSB). Interventions with respects to total cost, norms, indoor air quality, reliability, supply security, required work, could be explored using the model. For instance, the model demonstrates that a considerable total cost (investment and operating cost) increase of electric heating and heat pump, rather than a reduction of wood pellet heating's total cost, are required to initiate and speed up wood pellet adoption. (Author)

  16. Household response to environmental incentives for rain garden adoption

    Science.gov (United States)

    Newburn, David A.; Alberini, Anna

    2016-02-01

    A decentralized approach to encourage the voluntary adoption of household stormwater management practices is increasingly needed to mitigate urban runoff and to comply with more stringent water quality regulations. We analyze the household response to a hypothetical rebate program to incentivize rain garden adoption using household survey data from the Baltimore-Washington corridor. We asked respondents whether the household would adopt a rain garden without a rebate or when offered a randomly assigned rebate. An interval-data model is used to estimate household demand on the willingness to pay (WTP) for a rain garden as a function of demographic factors, gardening activities, environmental attitudes, and other household characteristics. Estimation results indicate that mean WTP for a rain garden in our sample population is approximately $6.72 per square foot, corresponding to almost three-fourths of the installation cost. The expected adoption rate more than tripled when comparing no rebate versus a government rebate set at one-third of the installation cost, indicating that economic incentives matter. There is substantial heterogeneity in the WTP among households. Higher levels of WTP are estimated for households with higher environmental concern for the Chesapeake Bay and local streams, garden experience, higher income, and non-senior citizen adults. We conclude that a cost-share rebate approach is likely to significantly affect household adoption decisions, and the partial contributions paid by households can assist with lowering the substantial compliance costs for local governments to meet water quality requirements.

  17. Negative outcomes of interethnic adoption of Mexican American children.

    Science.gov (United States)

    Bausch, R S; Serpe, R T

    1997-03-01

    This article identifies concerns about four possible negative outcomes of interethnic adoption involving Mexican American children and non-Mexican American parents. A sample of 861 Mexican Americans age 18 or older were asked whether they agreed or disagreed that four outcomes result from interethnic adoption: (1) The child may have an ethnic identity conflict, (2) the child may forget his or her Latino background, (3) the child's participation in Latino cultural events may be limited, and (4) the child may not acquire the skills to cope with racism. Respondents' agreement with the likelihood of these outcomes was associated with a belief in the importance of structural and cultural barriers preventing Latinos from adopting; with higher levels of participation in Mexican American cultural events; and with income, education, and acculturation. However, agreement that the outcomes were likely did not necessarily reflect approval or disapproval of interethnic adoption. Suggestions are made for future research on Mexican American children adopted by non-Mexican American parents.

  18. Cloud Computing Adoption Model for Universities to Increase ICT Proficiency

    Directory of Open Access Journals (Sweden)

    Safiya Okai

    2014-08-01

    Full Text Available Universities around the world especially those in developing countries are faced with the problem of delivering the level of information and communications technology (ICT needed to facilitate teaching, learning, research, and development activities ideal in a typical university, which is needed to meet educational needs in-line with advancement in technology and the growing dependence on IT. This is mainly due to the high cost involved in providing and maintaining the needed hardware and software. A technology such as cloud computing that delivers on demand provisioning of IT resources on a pay per use basis can be used to address this problem. Cloud computing promises better delivery of IT services as well as availability whenever and wherever needed at reduced costs with users paying only as much as they consume through the services of cloud service providers. The cloud technology reduces complexity while increasing speed and quality of IT services provided; however, despite these benefits the challenges that come with its adoption have left many sectors especially the higher education skeptical in committing to this technology. This article identifies the reasons for the slow rate of adoption of cloud computing at university level, discusses the challenges faced and proposes a cloud computing adoption model that contains strategic guidelines to overcome the major challenges identified and a roadmap for the successful adoption of cloud computing by universities. The model was tested in one of the universities and found to be both useful and appropriate for adopting cloud computing at university level.

  19. Understanding Social Contagion in Adoption Processes Using Dynamic Social Networks.

    Science.gov (United States)

    Herrera, Mauricio; Armelini, Guillermo; Salvaj, Erica

    2015-01-01

    There are many studies in the marketing and diffusion literature of the conditions in which social contagion affects adoption processes. Yet most of these studies assume that social interactions do not change over time, even though actors in social networks exhibit different likelihoods of being influenced across the diffusion period. Rooted in physics and epidemiology theories, this study proposes a Susceptible Infectious Susceptible (SIS) model to assess the role of social contagion in adoption processes, which takes changes in social dynamics over time into account. To study the adoption over a span of ten years, the authors used detailed data sets from a community of consumers and determined the importance of social contagion, as well as how the interplay of social and non-social influences from outside the community drives adoption processes. Although social contagion matters for diffusion, it is less relevant in shaping adoption when the study also includes social dynamics among members of the community. This finding is relevant for managers and entrepreneurs who trust in word-of-mouth marketing campaigns whose effect may be overestimated if marketers fail to acknowledge variations in social interactions.

  20. Understanding Social Contagion in Adoption Processes Using Dynamic Social Networks.

    Directory of Open Access Journals (Sweden)

    Mauricio Herrera

    Full Text Available There are many studies in the marketing and diffusion literature of the conditions in which social contagion affects adoption processes. Yet most of these studies assume that social interactions do not change over time, even though actors in social networks exhibit different likelihoods of being influenced across the diffusion period. Rooted in physics and epidemiology theories, this study proposes a Susceptible Infectious Susceptible (SIS model to assess the role of social contagion in adoption processes, which takes changes in social dynamics over time into account. To study the adoption over a span of ten years, the authors used detailed data sets from a community of consumers and determined the importance of social contagion, as well as how the interplay of social and non-social influences from outside the community drives adoption processes. Although social contagion matters for diffusion, it is less relevant in shaping adoption when the study also includes social dynamics among members of the community. This finding is relevant for managers and entrepreneurs who trust in word-of-mouth marketing campaigns whose effect may be overestimated if marketers fail to acknowledge variations in social interactions.

  1. Assessing integrated pest management adoption: measurement problems and policy implications.

    Science.gov (United States)

    Puente, Molly; Darnall, Nicole; Forkner, Rebecca E

    2011-11-01

    For more than a decade, the U.S. government has promoted integrated pest management (IPM) to advance sustainable agriculture. However, the usefulness of this practice has been questioned because of lagging implementation. There are at least two plausible rationales for the slow implementation: (1) growers are not adopting IPM-for whatever reason-and (2) current assessment methods are inadequate at assessing IPM implementation. Our research addresses the second plausibility. We suggest that the traditional approach to measuring IPM implementation on its own fails to assess the distinct, biologically hierarchical components of IPM, and instead aggregates growers' management practices into an overall adoption score. Knowledge of these distinct components and the extent to which they are implemented can inform government officials as to how they should develop targeted assistance programs to encourage broader IPM use. We address these concerns by assessing the components of IPM adoption and comparing our method to the traditional approach alone. Our results indicate that there are four distinct components of adoption-weed, insect, general, and ecosystem management-and that growers implement the first two components significantly more often than the latter two. These findings suggest that using a more nuanced measure to assess IPM adoption that expands on the traditional approach, allows for a better understanding of the degree of IPM implementation.

  2. Mobile Data Technology for Small Businesses: needs, uses and adoption

    Directory of Open Access Journals (Sweden)

    Debra Harker

    2002-05-01

    Full Text Available The technological environment in which Australian SMEs operate can be best described as dynamic and vital. The rate of technological change provides the SME owner/manger a complex and challenging operational context. Wireless applications are being developed that provide mobile devices with Internet content and E-business services. In Australia, the adoption of commerce by large organisations has been relatively high, however, the same cannot be said for SMEs, where adoption has been slower than other developed countries. In contrast, however, mobile telephone adoption and diffusion is relatively high by SMEs. Will SMEs who have been reluctant to adopt ecommerce technologies in the past be more ready to go on-line with the merging of the Internet and mobile data technologies? This exploratory study identifies attitudes, perceptions and issues for mobile data technologies by regional SME owner/managers across a range of industry sectors. The major issues include the sector the firm belongs to, the current adoption status of the firm, the level of mistrust of the IT industry, the cost of the technologies, and the applications and attributes of the technologies.

  3. Barriers to E-Commerce Adoption in Egyptian SMEs

    Directory of Open Access Journals (Sweden)

    Abdel Nasser H. Zaied

    2012-07-01

    Full Text Available E-commerce has been predicted to be a new driver of economic growth for developing countries. The SME sector plays a significant role in its contribution to the national economy in terms of the wealth created and the number of people employed. Small and Medium Enterprises (SMEs in Egypt represent the greatest share of the productive units of the Egyptian economy and the current national policy directions address ways and means of developing the capacities of SMEs. Many factors could be responsible for the low usage of e-commerce among the SMEs in Egypt. In order to determine the factors that promote the adoption of e-commerce, SMEs adopters and non-adopters of e-commerce were asked to indicate the factors inhibiting the adoption of e-commerce. The results show that technical barriers are the most important barriers followed by legal and regulatory barriers, whereas lack of Internet security is the highest barrier that inhibit the implementation of e-commerce in SMEs in Egypt followed by limited use of Internet banking and web portals by SMEs. Also, findings implied that more efforts are needed to help and encourage SMEs in Egypt to speed up e-commerce adoption, particularly the more advanced applications.

  4. Why, when, and how fast innovations are adopted

    CERN Document Server

    Goncalves, Sebastian; Iglesias, J R; 10.1140/epjb/e2012-30082-6

    2012-01-01

    When the full stock of a new product is quickly sold in a few days or weeks, one has the impression that new technologies develop and conquer the market in a very easy way. This may be true for some new technologies, for example the cell phone, but not for others, like the blue-ray. Novelty, usefulness, advertising, price, and fashion are the driving forces behind the adoption of a new product. But, what are the key factors that lead to adopt a new technology? In this paper we propose and investigate a simple model for the adoption of an innovation which depends mainly on three elements: the appeal of the novelty, the inertia or resistance to adopt it, and the interaction with other agents. Social interactions are taken into account in two ways: by imitation and by differentiation, i.e., some agents will be inclined to adopt an innovation if many people do the same, but other will act in the opposite direction, trying to differentiate from the "herd". We determine the conditions for a successful implantation ...

  5. Understanding Social Contagion in Adoption Processes Using Dynamic Social Networks

    Science.gov (United States)

    2015-01-01

    There are many studies in the marketing and diffusion literature of the conditions in which social contagion affects adoption processes. Yet most of these studies assume that social interactions do not change over time, even though actors in social networks exhibit different likelihoods of being influenced across the diffusion period. Rooted in physics and epidemiology theories, this study proposes a Susceptible Infectious Susceptible (SIS) model to assess the role of social contagion in adoption processes, which takes changes in social dynamics over time into account. To study the adoption over a span of ten years, the authors used detailed data sets from a community of consumers and determined the importance of social contagion, as well as how the interplay of social and non-social influences from outside the community drives adoption processes. Although social contagion matters for diffusion, it is less relevant in shaping adoption when the study also includes social dynamics among members of the community. This finding is relevant for managers and entrepreneurs who trust in word-of-mouth marketing campaigns whose effect may be overestimated if marketers fail to acknowledge variations in social interactions. PMID:26505473

  6. Adoption of Innovative Information Systems by SMEs: Comparing The Role of Firm’s Enacted Capabilities of Active Adopters and Non-Active Adopters

    Directory of Open Access Journals (Sweden)

    Noor Akma Mohd Salleh

    2007-10-01

    Full Text Available The adoption of innovative information systems (IS by small-and medium-sized enterprises (SMEs across heterogeneous culture, locales, and markets are a critical and an ongoing challenge. Such challenge requires more than just good ideas and extensive resources. It requires organisational capabilities that can be labelled as “enacted capabilities”. This study defines enacted capabilities as the firm’s ability to mobilise and deploy IS-based resources in combination or co-present with other capabilities within SMEs. Consequently, the aim of this study is to make a contribution by empirically examining the enacted capabilities of SMEs in developing countries that may influence the success of innovative IS adoption. In line with this objective, an innovative IS adoption behaviour investigation is conducted particularly as to why some SMEs are able to be enabled for use and utilise innovative IS, while others fail to do so. A survey of 206 of the CEOs/owners from Malaysian SMEs was con-ducted. The innovative IS examined was the government’s electronic procure-ment systems. The findings are consistent with the notion that all SMEs have enacted capabilities. Some SMEs integrate and coordinate them in a different way, depending on the context of each organisation. The findings also indicate that strong enacted capabilities and perceived net benefits affect the SMEs’ ability to perform or assimilate IS related strategic change.

  7. Factors affecting adoption of mobile banking in Pakistan: Empirical Evidence

    Directory of Open Access Journals (Sweden)

    Abdul Kabeer Kazi

    2013-07-01

    Full Text Available In this research paper we investigated the determinants likely to influence the adoption of mobile banking services, with a special focus on under banked/unbanked low-income population of Pakistan. For this purpose, Technology Acceptance Model (TAM was used, with additional determinants of perceived risk and social influence. We designed a questionnaire for this research; data was collected by surveying 372 respondents from the two largest cities (Karachi and Hyderabad of the province Sindh, in Pakistan. This study empirically concluded that consumers’ intention to adopt mobile banking services was significantly influenced by social influence, perceived risk, perceived usefulness, and perceived ease of use. The most significant positive influence was of social influence on consumers’ intention to adopt mobile banking services. The paper concluded with discussion on results, and several business implications for the banking industry of Pakistan.

  8. Policy Incentives for the Adoption of Electric Vehicles across Countries

    Directory of Open Access Journals (Sweden)

    Xingping Zhang

    2014-11-01

    Full Text Available Electric vehicles (EVs have prominent advantages for reducing CO2 emissions and alleviating the dependence on fossil fuel consumption in the transport sector. Therefore, many countries have set targets for EV development in recent years and have employed a number of policies to achieve environmental objectives and alleviate the energy pressure. Despite the fact that the adoption of EVs has increased in the past few years, more policies, such as financial incentives, technology support or charging infrastructure, should be made by governments to promote broader range use of EVs. In this paper, we review the relevant policies that different countries may adopt for stimulating the market of EVs. Based on this, we analyze the relationship between the policies and the adoption of EVs by taking America as an example. In conclusion, some effective policies are summarized to spur the market. Therefore, each country should learn from each other and employ effective policies based on the actual situation.

  9. Factors influencing adoption of manure separation technology in The Netherlands.

    Science.gov (United States)

    Gebrezgabher, Solomie A; Meuwissen, Miranda P M; Kruseman, Gideon; Lakner, Dora; Oude Lansink, Alfons G J M

    2015-03-01

    Manure separation technologies are essential for sustainable livestock operations in areas with high livestock density as these technologies result in better utilization of manure and reduced environmental impact. Technologies for manure separation have been well researched and are ready for use. Their use, however, has been limited to the Netherlands. This paper investigates the role of farm and farmer characteristics and farmers' attitudes toward technology-specific attributes in influencing the likelihood of the adoption of mechanical manure separation technology. The analysis used survey data collected from 111 Dutch dairy farmers in 2009. The results showed that the age and education level of the farmer and farm size are important variables explaining the likelihood of adoption. In addition to farm and farmer characteristics, farmers' attitudes toward the different attributes of manure separation technology significantly affect the likelihood of adoption. The study generates useful information for policy makers, technology developers and distributors in identifying the factors that impact decision-making behaviors of farmers.

  10. Which Factors Influence the Adoption of Social Software?

    DEFF Research Database (Denmark)

    Mukkamala, Alivelu Manga; Razmerita, Liana

    2014-01-01

    method approach has been applied, and drawing on social dilemma theory and Hofstede’s cultural theory, this study discusses the factors affecting the adoption of social software by knowledge workers. A quantitative descriptive-explanatory study and a qualitative exploratory study have been employed......The rationale behind traditional knowledge management initiatives is to create, capture, share, organize, and use intangible assets such as organizational knowledge. Information systems have been playing a vital role in the implementation of knowledge management practices and systems. Recently......, organizations are adopting new forms of information and communication technologies such as social software to encourage employees to create and share knowledge. This article explores the adoption of social software tools by Indian knowledge workers working for information technology consultancy firms. A mixed...

  11. Adoptive transfer of macrophages ameliorates renal fibrosis in mice.

    Science.gov (United States)

    Nishida, Masashi; Okumura, Yasuko; Fujimoto, Shin-Ichiro; Shiraishi, Isao; Itoi, Toshiyuki; Hamaoka, Kenji

    2005-06-24

    We performed adoptive transfer of bone marrow-derived (BM) macrophages following pharmacological depletion of leukocytes in a mouse model of unilateral ureteral obstruction (UUO). Treatment with cyclophosphamide (CPM) caused marked decrease in the numbers of F4/80-positive interstitial macrophages as well as in peripheral blood leukocyte counts, and adoptive transfer of BM macrophages to CPM-treated mice resulted in significant increase in the numbers of interstitial macrophages both at day 5 and at day 14 after UUO. At day 5 after UUO, no significant change was observed in the degree of renal interstitial fibrosis either by treatment with CPM or with CPM+macrophage. However, at day 14 after UUO, treatment with CPM caused significant increase in the degree of interstitial fibrosis, and adoptive macrophage transfer to these mice attenuated this enhancement in renal fibrosis. Our result suggests the role of infiltrating macrophages on facilitating tissue repair at late stage of UUO.

  12. 'School adopts an experiment': the magnetic levitation of superconductors

    Science.gov (United States)

    Agliolo Gallitto, Aurelio

    2010-09-01

    The event 'School adopts an experiment' is an event targeted at high schools and secondary schools. It is based on a tight and direct collaboration between researchers and school students and teachers. Several schools were involved in the event by 'adopting' an experiment in physics research laboratories. Groups of selected students were first trained by university researchers, then they demonstrated the experiments to other students and teachers during the event. Students who adopted an experiment acquired knowledge of the subjects connected with the experiment in a most impressive way compared with the usual didactics at school. Further positive points were: (i) to establish a close and direct collaboration between university researchers and high-school students and teachers; (ii) to give school students the opportunity to visit physics research laboratories.

  13. Resistance to Mobile Banking Adoption in Egypt: A Cultural Perspective

    Directory of Open Access Journals (Sweden)

    Rehaballah Elbadrawy

    2011-12-01

    Full Text Available Mobile banking (m-banking faces various types of resistance that may hinder customers’ adoption inEgypt. This study identifies three groups of m-banking non-adopters, namely postponers, opponents andrejectors. The objective of the study is to explore the reasons for resisting m-banking services in Egypt andwhether it differs with regards to these customer groups. Accordingly, a questionnaire was distributed, Chisquare tests, Kruskal-Wallis H tests and one-way analysis of variance (ANOVA test. Frequencies andcross tabulations were used. The results indicate that the three non-adopter groups differ significantly withrespect to usage, value, and image barriers. On the other hand, risk and tradition barriers did not showany statistical significance; however, risk barrier received the highest overall mean. Significant relationsbetween usage, risk and image barriers with the gender and level of education were noted. Finally, findingsenabled a clear mapping between Hofstede’s cultural dimensions and the study’s results.

  14. Transaction Costs: Prosecuting child trafficking for illegal adoption in Russia

    Directory of Open Access Journals (Sweden)

    Lauren A McCarthy

    2016-05-01

    Full Text Available As primary implementers of laws on human trafficking, law enforcement helps construct how these laws are understood and applied. This article examines how this process has unfolded in Russia by looking at the phenomenon of and debates surrounding child trafficking for illegal adoption. It argues that pre-existing experience with trafficking laws and cultural narratives surrounding adoption have led law enforcement to focus on uncovering evidence of monetary transactions rather than exploitation when prosecuting trafficking cases. This construction of the meaning of trafficking comes with important trade-offs. While the emphasis on transactions helps law enforcement to be successful at prosecuting cases involving selling children for illegal adoption, a focus on transactions rather than exploitation results in a de facto prosecution policy that ignores the many forms of exploitation that occur in other trafficking cases.

  15. Optimal Rules to Adopt High Technology under Uncertainty

    Institute of Scientific and Technical Information of China (English)

    ZHANG Xiao-jun; LI Shi-min

    2006-01-01

    In the research of choosing the optimal timing for the high technology products,especially IT products to the market, most studies prefer to provide the scope or infimum of timing. In this paper, an optimal rule is adopted to guild the timing of high technology product to the market, this idea is illustrated through the theory of optimal stopping, and a high approach is developed to theoretical framework for timing decision. On this basis, a random programming model is established, in which the objective function is the expected profit to adopt high technology and the constraint condition is the successful probability over critical value α with all variables beyond the rule, and it is used to find the optimal timing of adopt high technology product.

  16. Small Firm Internet Adoption: a market oriented approach

    Directory of Open Access Journals (Sweden)

    Colin Jones

    2002-11-01

    Full Text Available Fundamental to the development of new customer value offerings via web-based commerce is a small firm’s ability to strategically acquire and exploit knowledge. The focus of this paper is the empirical testing of a normative web-based commerce adoption model developed from a review of the extant literature related to electronic marketing, the Internet and the diffusion of new innovations. A preliminary test of the model’s theoretical contentions lent support to its overall focus, but found that the firm’s existing learning capabilities were diminished during the adoption of web-based commerce. Consequently, sub-optimal adoption outcomes were associated with insufficient knowledge development.

  17. A Model of U.S. Commercial Distributed Generation Adoption

    Energy Technology Data Exchange (ETDEWEB)

    LaCommare, Kristina Hamachi; Ryan Firestone; Zhou, Nan; Maribu,Karl; Marnay, Chris

    2006-01-10

    Small-scale (100 kW-5 MW) on-site distributed generation (DG) economically driven by combined heat and power (CHP) applications and, in some cases, reliability concerns will likely emerge as a common feature of commercial building energy systems over the next two decades. Forecasts of DG adoption published by the Energy Information Administration (EIA) in the Annual Energy Outlook (AEO) are made using the National Energy Modeling System (NEMS), which has a forecasting module that predicts the penetration of several possible commercial building DG technologies over the period 2005-2025. NEMS is also used for estimating the future benefits of Department of Energy research and development used in support of budget requests and management decisionmaking. The NEMS approach to modeling DG has some limitations, including constraints on the amount of DG allowed for retrofits to existing buildings and a small number of possible sizes for each DG technology. An alternative approach called Commercial Sector Model (ComSeM) is developed to improve the way in which DG adoption is modeled. The approach incorporates load shapes for specific end uses in specific building types in specific regions, e.g., cooling in hospitals in Atlanta or space heating in Chicago offices. The Distributed Energy Resources Customer Adoption Model (DER-CAM) uses these load profiles together with input cost and performance DG technology assumptions to model the potential DG adoption for four selected cities and two sizes of five building types in selected forecast years to 2022. The Distributed Energy Resources Market Diffusion Model (DER-MaDiM) is then used to then tailor the DER-CAM results to adoption projections for the entire U.S. commercial sector for all forecast years from 2007-2025. This process is conducted such that the structure of results are consistent with the structure of NEMS, and can be re-injected into NEMS that can then be used to integrate adoption results into a full forecast.

  18. Hospital financial position and the adoption of electronic health records.

    Science.gov (United States)

    Ginn, Gregory O; Shen, Jay J; Moseley, Charles B

    2011-01-01

    The objective of this study was to examine the relationship between financial position and adoption of electronic health records (EHRs) in 2442 acute care hospitals. The study was cross-sectional and utilized a general linear mixed model with the multinomial distribution specification for data analysis. We verified the results by also running a multinomial logistic regression model. To measure our variables, we used data from (1) the 2007 American Hospital Association (AHA) electronic health record implementation survey, (2) the 2006 Centers for Medicare and Medicaid Cost Reports, and (3) the 2006 AHA Annual Survey containing organizational and operational data. Our dependent variable was an ordinal variable with three levels used to indicate the extent of EHR adoption by hospitals. Our independent variables were five financial ratios: (1) net days revenue in accounts receivable, (2) total margin, (3) the equity multiplier, (4) total asset turnover, and (5) the ratio of total payroll to total expenses. For control variables, we used (1) bed size, (2) ownership type, (3) teaching affiliation, (4) system membership, (5) network participation, (6) fulltime equivalent nurses per adjusted average daily census, (7) average daily census per staffed bed, (8) Medicare patients percentage, (9) Medicaid patients percentage, (10) capitation-based reimbursement, and (11) nonconcentrated market. Only liquidity was significant and positively associated with EHR adoption. Asset turnover ratio was significant but, unexpectedly, was negatively associated with EHR adoption. However, many control variables, most notably bed size, showed significant positive associations with EHR adoption. Thus, it seems that hospitals adopt EHRs as a strategic move to better align themselves with their environment.

  19. Growing the intention to adopt educational innovations: An empirical study

    Directory of Open Access Journals (Sweden)

    David M. Bourrie

    2016-03-01

    Full Text Available In order for the Open Access (OA to learning concept to a have wider impact in formal education, it is important that faculty members intent to adopt new educational innovations. However, little is known about which variables influence the intention of faculty members. Therefore, the purposes of this study are to empirically determine: 1 which of the characteristics of the educational innovation significantly influence the intention to adopt educational innovations, 2 which variables influence the readiness of faculty members intention to adopt educational innovations, and 3 how the characteristics of the innovations moderate the relationship between faculty readiness and intention to adopt the innovations. Participants of this study include 335 faculty members in ABET certified computer science and electrical engineering programs in the United States. The results show that ease of use is positively related to the intention of faculty members to adopt an educational innovation. We conclude that Open-CourseWare developers need to ensure that ease of use is emphasized in the CourseWare and they need to propagate these initially in institutions where faculty members have positive attitude to the CourseWare and care about student learning. In addition, a new method of identifying, building, and funding “open access grant” universities that develop easy-to-use educational innovations, make them available on an open access platform, and spread them widely by embedding agents in community colleges, schools, and other educational institutions is essential. Such an initiative may lead to wider adoption of MOOCS and other open access materials.

  20. Economic impacts of adoption and fundraising strategies in animal shelters.

    Science.gov (United States)

    Lord, Emily; Olynk Widmar, Nicole; Litster, Annette

    2014-03-01

    The adoption strategies used in animal shelters can have a large impact on the total number of adoptions and donations that take place. Reducing adoption fees during peak kitten or puppy season is one way to reduce inventories and increase the number of open spaces to save more lives, but does not necessarily increase the financial well-being of the shelter if the per-animal costs exceed the revenues generated. We developed a stochastic model to simulate the expected costs, revenues, and net income of a hypothetical animal shelter for various alternative management strategies, based on US conditions. A total of 8 scenarios were developed and compared to the base-case scenario (BC). In the model, scenarios which decreased or waived adoption fees caused total costs to increase due to the escalating costs associated with increasing the total number and density of animals housed. This effect was especially pronounced when adoptions were free. When the return on money invested in additional fundraising was predetermined to be 'good' (rather than 'fair' or 'poor'), net shelter income did exceed costs - but even 'fair' return increased net shelter income compared to the BC. Of the eight scenarios compared to BC, the mean monthly net income was significantly different from that in the BC in all eight scenarios (pfundraising. In these scenarios, because the extra cost involved is relatively low compared to the other scenarios, the potential risk of a reduction in net shelter revenue is reduced. When shelters are aware of the positive and negative impacts of various adoption strategies on mean net income and variation in net income, shelter managers can better strategize saving animal lives and meeting shelter goals, while maintaining the financial health and functionality of the operation.

  1. Performance assessment and adoption processes of an information monitoring and diagnostic system prototype

    Energy Technology Data Exchange (ETDEWEB)

    Piette, Mary Ann

    1999-10-01

    This report addresses the problem that buildings do not perform as well as anticipated during design. We partnered with an innovative building operator to evaluate a prototype Information Monitoring and Diagnostic System (IMDS). The IMDS consists of high-quality measurements archived each minute, a data visualization tool, and a web-based capability. The operators recommend similar technology be adopted in other buildings. The IMDS has been used to identify and correct a series of control problems. It has also allowed the operators to make more effective use of the building control system, freeing up time to take care of other tenant needs. They believe they have significantly improved building comfort, potentially improving tenant health, and productivity. The reduction in hours to operate the building are worth about $20,000 per year, which could pay for the IMDS in about five years. A control system retrofit based on findings from the IMDS is expected to reduce energy use by 20 percent over the next year, worth over $30,000 per year. The main conclusion of the model-based chiller fault detection work is that steady-state models can be used as reference models to monitor chiller operation and detect faults. The ability of the IMDS to measure cooling load and chiller power to one-percent accuracy with a one-minute sampling interval permits detection of additional faults. Evolutionary programming techniques were also evaluated, showing promise in the detection of patterns in building data. We also evaluated two technology adoption processes, radical and routine. In routine adoption, managers enhance features of existing products that are already well understood. In radical adoption, innovative building managers introduce novel technology into their organizations without using the rigorous payback criteria used in routine innovations.

  2. Catalyzing new product adoption at the base of the pyramid

    OpenAIRE

    Marinakis, Y.D.; Walsh, S. T.; Harms, R.

    2016-01-01

    One of the more perplexing of the entrepreneurial issues at the Base of the Pyramid (BoP) is how to catalyze new product adoption by BoP consumers. Because S-shaped adoption dynamics are the result of cultural transmission bias, the question can be rephrased as, how can an entrepreneur overcome conformity bias. We modified the Technology Acceptance Model (TAM) to include conformity bias. We then qualitatively applied the model to three examples from the literature, namely fuel stoves in Darfu...

  3. ADOPTABLE TECHNIQUE(S FOR MANAGING GHANAIAN SALINE SOILS

    Directory of Open Access Journals (Sweden)

    Akwasi Asamoah

    2013-03-01

    Full Text Available : Salinization of Ghanaian soils is on the rise. Organic matter application has not proved an effective and feasible technique for curbing this rise. Hence this paper seeks to review techniques that Ghana is using to manage its saline soils and further recommend a feasible, cost effective and beneficial technique for exhaustive research and possible adoption in the future. Halophytes appear to be the most feasible, cost effective and beneficial technique which could be adopted for the effective management of Ghanaian saline soils. But where halophytes are exotic, care must be taken to avoid competition with native species and allow preservation of agrobiodiversity

  4. Assessing the Role of Technology Adoption in China's Growth Performance

    DEFF Research Database (Denmark)

    Wirz, Nadja

    China has experienced a period of tremendous economic growth in recent years. In an attempt to explain this development, several existing growth-accounting studies reveal that impressively high rates of productivity growth have been at the heart of China's performance. This study investigates...... to what extent these productivity increases can be explained by technology-adoption theory. In less developed countries, the key element behind technological progress is technology adoption, the process of copying technological knowledge invented throughout the world. To uncover a measure of China...

  5. IFRS ADOPTION AROUND THE WORLD - A BRIEF LITERATURE REVIEW

    Directory of Open Access Journals (Sweden)

    ALINA RUSU

    2012-05-01

    Full Text Available Our paper aims to provide an updated description of the process of IFRS adoption in the E.U. and worldwide, pointing out its effects on the information presented in financial statements, on the markets efficiency and on the accounting harmonization. In order to achieve our goal, we have analyzed approximately 40 academic articles, published between 2000 and 2012, indexed in international databases, such as Science Direct, Emerald and ProQuest. Also, we have tried a classification of these papers according to the country analyzed in each of them, making a comparative analysis between the IFRS adoption effects in Code and Common Law countries.

  6. Exploiting cytokines in adoptive T-cell therapy of cancer.

    Science.gov (United States)

    Petrozziello, Elisabetta; Sturmheit, Tabea; Mondino, Anna

    2015-01-01

    Adoptive immunotherapy with tumor-reactive autologous T cells, either expanded from tumor specimens or genetically engineered to express tumor-reactive T-cell receptors and chimeric antigen receptors, is holding promising results in clinical trials. Several critical issues have been identified and results underline the possibility to exploit cytokines to further ameliorate the efficacy of current treatment protocols, also encompassing adoptive T-cell therapy. Here we review latest developments on the use of cytokines to better direct the nature of the T-cell infusion product, T-cell function and persistence in vivo, as well as to modulate the tumor microenvironment.

  7. Post-Adoption Contact, Adoption Communicative Openness, and Satisfaction with Contact as Predictors of Externalizing Behavior in Adolescence and Emerging Adulthood

    Science.gov (United States)

    Grotevant, Harold D.; Rueter, Martha; Von Korff, Lynn; Gonzalez, Christopher

    2011-01-01

    Background: This study examined the relation between three variables related to adoptive family relationships (post-adoption contact between adoptive and birth family members, adoption communicative openness, and satisfaction with contact) and adoptee externalizing behavior in adolescence and emerging adulthood. Method: The study included 190…

  8. Inherited and environmental influences on a childhood co-occurring symptom phenotype: Evidence from an adoption study.

    Science.gov (United States)

    Roos, Leslie E; Fisher, Philip A; Shaw, Daniel S; Kim, Hyoun K; Neiderhiser, Jenae M; Reiss, David; Natsuaki, Misake N; Leve, Leslie D

    2016-02-01

    Risk factors for the childhood development of co-occurring internalizing and externalizing symptoms are not well understood, despite a high prevalence and poor clinical outcomes associated with this co-occurring phenotype. We examined inherited and environmental risk factors for co-occurring symptoms in a sample of children adopted at birth and their birth mothers and adoptive mothers (N = 293). Inherited risk factors (i.e., birth mothers' processing speed and internalizing symptoms) and environmental risk factors (i.e., adoptive mothers' processing speed, internalizing symptoms, and uninvolved parenting) were examined as predictors for the development of internalizing-only, externalizing-only, or co-occurring symptoms using structural equation modeling. Results suggested a unique pattern of predictive factors for the co-occurring phenotype, with risk conferred by adoptive mothers' uninvolved parenting, birth mothers' slower processing speed, and the birth mothers' slower processing speed in tandem with adoptive mothers' higher internalizing symptoms. Additional analyses indicated that when co-occurring-symptom children were incorporated into internalizing and externalizing symptom groups, differential risk factors for externalizing and internalizing symptoms emerged. The findings suggest that spurious results may be found when children with co-occurring symptoms are not examined as a unique phenotypic group.

  9. Java enterprise design patterns patterns in Java

    CERN Document Server

    Grand, Mark

    2002-01-01

    A how-to guide for Java programmers who want to use design patterns when developing real-world enterprise applicationsThis practical book explores the subject of design patterns, or patterns that occur in the design phase of a project''s life cycle. With an emphasis on Java for the enterprise, Mark Grand guides Java programmers on how to apply traditional and new patterns when designing a large enterprise application. The author clearly explains how existing patterns work with the new enterprise design patterns and demonstrates through case studies how to use design patterns in the real world.

  10. The adoption of mobile health management services: an empirical study.

    Science.gov (United States)

    Hung, Ming-Chien; Jen, Wen-Yuan

    2012-06-01

    As their populations age, many countries are facing the increasing economic pressure of providing healthcare to their people. In Taiwan, this problem is exacerbated by an increasing rate of obesity and obesity-related conditions. Encouraging the adoption of personal health management services is one way to maintain current levels of personal health and to efficiently manage the distribution of healthcare resources. This study introduces Mobile Health Management Services (MHMS) and employs the Technology Acceptance Model (TAM) to explore the intention of students in Executive Master of Business Management programs to adopt mobile health management technology. Partial least squares (PLS) was used to analyze the collected data, and the results revealed that "perceived usefulness" and "attitude" significantly affected the behavioral intention of adopting MHMS. Both "perceived ease of use" and "perceived usefulness," significantly affected "attitude," and "perceived ease of use" significantly affected "perceived usefulness" as well. The results also show that the determinants of intention toward MHMS differed with age; young adults had higher intention to adopt MHMS to manage their personal health. Therefore, relevant governmental agencies may profitably promote the management of personal health among this population. Successful promotion of personal health management will contribute to increases in both the level of general health and the efficient management of healthcare resources.

  11. Hospital marketing and the Internet: the adoption of an innovation.

    Science.gov (United States)

    Shepherd, C David; Fell, Daniel

    2003-01-01

    Given the manner in which the Internet permeates today's society, it is easy to forget that the Internet as we know it is only about a decade old. Due to its newness, the Internet has provided an opportunity to view the manner in which marketers move to adopt such an innovation. To that end, since 1995 a series of studies has tracked the adoption and use of the Internet by hospital marketers. In addition to simply investigating the number of hospitals that utilize the Internet in their marketing efforts, the studies have addressed such questions as: How are hospitals using the Internet to market themselves?, What roadblocks have held hospitals back from adopting the Internet as a marketing tool?, and How happy are hospitals with their Internet investment? This manuscript details the most recent in this series of research studies. In addition, the authors compare the results of this study, conducted in the fall of 2002, with the results from the previous studies. As a result, this study provides a unique opportunity to observe the actual adoption of an innovation, the Internet, by hospital marketing professionals.

  12. Predicting early adoption of successive video player generations

    NARCIS (Netherlands)

    Rijnsoever, F.J. van; Oppewal, H.

    2012-01-01

    Early adopters play an important role in the innovation diffusion process. Over the past decades, many factors have been identified as predictors for earlyadoption of innovations. Less attention has been paid to the relationship between the earlyadoption of one generation of a specific product and t

  13. The websites adoption in the Spanish agrifood firms

    Directory of Open Access Journals (Sweden)

    Erasmo I. López-Becerra

    2016-12-01

    Full Text Available Currently, the use of websites in the firms of the Spanish agrifood sector has not become widespread despite the possible benefits to be derived from their adequate integration and management within the firms. Among these advantages, the increase in the quality of the services offered and the improvement in the relations with their stakeholders should be highlighted. In this context, the objective of the present work is to determine to what extent websites are used by firms in the agrifood sector of Spain, analysing the background which explains why they are adopted and the consequences of introducing them. To do so, a theoretical framework is posed which allows these factors to be identified, and an empirical study is carried out with Spanish agrifood firms. The results of the work indicate that the adoption of websites will be favoured when the directors of the firm are higher qualified, the firm is bigger in size, the firm’s function is distribution, and when the relative advantage and technology compatibility is perceived, among others. Technology adoption consequences analysis suggests that the benefits of adoption are related with improving the relationships with suppliers, customers, owning partners and, in general, the quality of the services offered by the firm.

  14. Rationale of Early Adopters of Fossil Fuel Divestment

    Science.gov (United States)

    Beer, Christopher Todd

    2016-01-01

    Purpose: This research uses the social science perspectives of institutions, ecological modernization and social movements to analyze the rationale used by the early-adopting universities of fossil fuel divestment in the USA. Design/methodology/approach: Through analysis of qualitative data from interviews with key actors at the universities that…

  15. India: a Case of Fragile Wireless Service and Technology Adoption?

    NARCIS (Netherlands)

    L-F. Pau (Louis-François); J. Motiwalla

    2007-01-01

    textabstractWireless penetration and the Indian economy have grown significantly over the past few years, but how robust and sustainable is the adoption of wireless services and products? Several papers have discussed India as a wireless service and product market, and sometimes tried to assess quan

  16. Significant Factors Determining E-government Adoption in Selangor, Malaysia

    Directory of Open Access Journals (Sweden)

    Siti Hajar Mohd Idris

    2016-06-01

    Full Text Available Studies have shown that low adoption rate among citizens has been hindering the optimization of e-Government services especially in developing countries. Hence, one of the critical measures that has to be undertaken is to identify and overcome possible barriers to further facilitate a higher rate of adoption. A multistage stratified sampling was used in this study to collect data from 1000 respondents, both user and non-user residing in the state of Selangor, Malaysia. This state was chosen as to provide a better understanding of low adoption when issues of basic facilities have been successfully overcome. An exploratory factor analysis was performed to identify latent constructs and seven key factors were identified. A multiple regression model was subsequently used to analyze significant factors in determining the willingness to use e-Government services. The determinants are language barrier, educational level, secure, format, easy to use, enjoyable, reliable, visual appeal and infrastructure. The result shows significant variables that act as barriers to adoption are reliable, enjoyable, easy to use, secure, and language used. The constraints pointed out in the open ended questions mainly focus on the issue of accessibility, ease of use and awareness. Overcoming these obstacles is therefore crucial in order to enhance the usage of e-Government services which consequently will improve the quality of public administration in Malaysia.

  17. Mobile ICT use in early adopter vs. late majority countries

    DEFF Research Database (Denmark)

    Gimpel, Gregory; Sudzina, Frantisek; Petrovcikova, Katarina

    2016-01-01

    The rapid global diffusion of smartphones has not been uniform. This study uses Triandis’ theory of interpersonal behaviour to investigate what drives smartphone use in early adopter (USA) versus late majority (Slovakia) countries. By surveying both current and potential owners, we also revisit K...

  18. 77 FR 62431 - Adoption of Updated EDGAR Filer Manual

    Science.gov (United States)

    2012-10-15

    ... for the updated Filer Manual and the rule amendments is October 15, 2012. In accordance with the APA... publication of these rules is necessary to coordinate the effectiveness of the updated Filer Manual with the... COMMISSION 17 CFR Part 232 Adoption of Updated EDGAR Filer Manual AGENCY: Securities and Exchange...

  19. 76 FR 73506 - Adoption of Updated EDGAR Filer Manual

    Science.gov (United States)

    2011-11-29

    ... practice, publication for notice and comment is not required under the Administrative Procedure Act (APA... COMMISSION 17 CFR Part 232 Adoption of Updated EDGAR Filer Manual AGENCY: Securities and Exchange Commission... revisions to the Electronic Data Gathering, Analysis, and Retrieval System (EDGAR) Filer Manual to...

  20. 78 FR 60684 - Adoption of Updated EDGAR Filer Manual

    Science.gov (United States)

    2013-10-02

    ..., publication for notice and comment is not required under the Administrative Procedure Act (APA).\\6\\ It follows... COMMISSION 17 CFR Part 232 Adoption of Updated EDGAR Filer Manual AGENCY: Securities and Exchange Commission... revisions to the Electronic Data Gathering, Analysis, and Retrieval System (EDGAR) Filer Manual and...