WorldWideScience

Sample records for alkaline hydrothermal vent

  1. The Origin of Life in Alkaline Hydrothermal Vents.

    Science.gov (United States)

    Sojo, Victor; Herschy, Barry; Whicher, Alexandra; Camprubí, Eloi; Lane, Nick

    2016-02-01

    Over the last 70 years, prebiotic chemists have been very successful in synthesizing the molecules of life, from amino acids to nucleotides. Yet there is strikingly little resemblance between much of this chemistry and the metabolic pathways of cells, in terms of substrates, catalysts, and synthetic pathways. In contrast, alkaline hydrothermal vents offer conditions similar to those harnessed by modern autotrophs, but there has been limited experimental evidence that such conditions could drive prebiotic chemistry. In the Hadean, in the absence of oxygen, alkaline vents are proposed to have acted as electrochemical flow reactors, in which alkaline fluids saturated in H2 mixed with relatively acidic ocean waters rich in CO2, through a labyrinth of interconnected micropores with thin inorganic walls containing catalytic Fe(Ni)S minerals. The difference in pH across these thin barriers produced natural proton gradients with equivalent magnitude and polarity to the proton-motive force required for carbon fixation in extant bacteria and archaea. How such gradients could have powered carbon reduction or energy flux before the advent of organic protocells with genes and proteins is unknown. Work over the last decade suggests several possible hypotheses that are currently being tested in laboratory experiments, field observations, and phylogenetic reconstructions of ancestral metabolism. We analyze the perplexing differences in carbon and energy metabolism in methanogenic archaea and acetogenic bacteria to propose a possible ancestral mechanism of CO2 reduction in alkaline hydrothermal vents. Based on this mechanism, we show that the evolution of active ion pumping could have driven the deep divergence of bacteria and archaea. PMID:26841066

  2. RNA Oligomerization in Laboratory Analogues of Alkaline Hydrothermal Vent Systems.

    Science.gov (United States)

    Burcar, Bradley T; Barge, Laura M; Trail, Dustin; Watson, E Bruce; Russell, Michael J; McGown, Linda B

    2015-07-01

    Discovering pathways leading to long-chain RNA formation under feasible prebiotic conditions is an essential step toward demonstrating the viability of the RNA World hypothesis. Intensive research efforts have provided evidence of RNA oligomerization by using circular ribonucleotides, imidazole-activated ribonucleotides with montmorillonite catalyst, and ribonucleotides in the presence of lipids. Additionally, mineral surfaces such as borates, apatite, and calcite have been shown to catalyze the formation of small organic compounds from inorganic precursors (Cleaves, 2008 ), pointing to possible geological sites for the origins of life. Indeed, the catalytic properties of these particular minerals provide compelling evidence for alkaline hydrothermal vents as a potential site for the origins of life since, at these vents, large metal-rich chimney structures can form that have been shown to be energetically favorable to diverse forms of life. Here, we test the ability of iron- and sulfur-rich chimneys to support RNA oligomerization reactions using imidazole-activated and non-activated ribonucleotides. The chimneys were synthesized in the laboratory in aqueous "ocean" solutions under conditions consistent with current understanding of early Earth. Effects of elemental composition, pH, inclusion of catalytic montmorillonite clay, doping of chimneys with small organic compounds, and in situ ribonucleotide activation on RNA polymerization were investigated. These experiments, under certain conditions, showed successful dimerization by using unmodified ribonucleotides, with the generation of RNA oligomers up to 4 units in length when imidazole-activated ribonucleotides were used instead. Elemental analysis of the chimney precipitates and the reaction solutions showed that most of the metal cations that were determined were preferentially partitioned into the chimneys. PMID:26154881

  3. From Geochemistry to Biochemistry: Simulating Prebiotic Chemistry Driven by Geochemical Gradients in Alkaline Hydrothermal Vents

    Science.gov (United States)

    Barge, Laurie

    2016-07-01

    Planetary water-rock interfaces generate energy in the form of redox, pH, and thermal gradients, and these disequilibria are particularly focused in hydrothermal vent systems where the reducing, heated hydrothermal fluid feeds back into the more oxidizing ocean. Alkaline hydrothermal vents have been proposed as a likely location for the origin of life on the early Earth due to various factors: including the hydrothermal pH / Eh gradients that resemble the ubiquitous electrical / proton gradients in biology, the catalytic hydrothermal precipitates that resemble inorganic catalysts in enzymes, and the presence of electron donors and acceptors in hydrothermal systems (e.g. H2 + CH4 and CO2) that are thought to have been utilized in the earliest metabolisms. Of particular importance for the emergence of metabolism are the mineral "chimneys" that precipitate at the vent fluid / seawater interface. Hydrothermal chimneys are flow-through chemical reactors that form porous and permeable inorganic membranes transecting geochemical gradients; in some ways similar to biological membranes that transect proton / ion gradients and harness these disequilibria to drive metabolism. These emergent chimney structures in the far-from-equilibrium system of the alkaline vent have many properties of interest to the origin of life that can be simulated in the laboratory: for example, they can generate electrical energy and drive redox reactions, and produce catalytic minerals (in particular the metal sulfides and iron oxyhydroxides - "green rust") that can facilitate chemical reactions towards proto-metabolic cycles and biosynthesis. Many of the factors prompting interest in alkaline hydrothermal vents on Earth may also have been present on early Mars, or even presently within icy worlds such as Europa or Enceladus - thus, understanding the disequilibria and resulting prebiotic chemistry in these systems can be of great use in assessing the potential for other environments in the Solar

  4. Free energy distribution and hydrothermal mineral precipitation in Hadean submarine alkaline vent systems: Importance of iron redox reactions under anoxic conditions

    Science.gov (United States)

    Shibuya, Takazo; Russell, Michael J.; Takai, Ken

    2016-02-01

    Thermodynamic calculations of mixing between hypothetical seawater and hydrothermal fluid in the Hadean deep ocean were carried out to predict saturation states of mineral precipitates and redox reactions that could occur in Hadean submarine alkaline hydrothermal systems associated with the serpentinization of ultramafic rocks. In the calculations, the seawater was assumed to be weakly acidic (pH = 5.5) and to include carbon dioxide, ferrous iron and silica, with or without nitrate, while the Hadean hydrothermal fluid was assumed to be highly alkaline (pH = 11) and to contain abundant molecular hydrogen, methane and bisulfide, based on the Archean geologic record, the modern low-temperature alkaline hydrothermal vent fluid (Lost City field), and experimental and theoretical considerations. The modeling indicates that potential mineral precipitates in the mixing zone (hydrothermal chimney structures) could consist mainly of iron sulfides but also of ferrous serpentine and brucite, siderite, and ferric iron-bearing minerals such as goethite, hematite and/or magnetite as minor phases. The precipitation of ferric iron-bearing minerals suggests that chemical iron oxidation would be made possible by pH shift even under anoxic condition. In the mixing zone, comprising an inorganic barrier precipitated at the interface of the two contrasting solutions, various redox reactions release free energy with the potential to drive endergonic reactions, assuming the involvement of coupling inorganic protoenzymes. Hydrogenotrophic methanogenesis and acetogenesis - long considered the most ancient forms of biological energy metabolisms - are able to achieve higher maximum energy yield (>0.5 kJ/kg hydrothermal fluid) than those in the modern serpentinization-associated seafloor hydrothermal systems (e.g., Kairei field). Furthermore, the recently proposed methanotrophic acetogenesis pathway was also thermodynamically investigated. It is known that methanotrophic acetogenesis would

  5. Evidence for Hydrothermal Vents as "Biogeobatteries" (Invited)

    Science.gov (United States)

    Nielsen, M. E.; Girguis, P. R.

    2010-12-01

    Hydrothermal vents are unique systems that play an important role in oceanic biogeochemical cycles. As chemically reduced hydrothermal fluid mixes with cold oxic seawater, minerals precipitate out of solution resulting in chimney structures composed largely of metal sulfides and anhydrite. Pyrite, which is a natural semi-conductor, is the primary sulfide mineral, but other minerals within chimneys are also conductive (e.g. chalcopyrite, wurtzite, and some iron oxides). Sulfide chimneys are also known to host an extensive endolithic microbial community. Accordingly, submarine hydrothermal systems appear to be examples of biogeobatteries, wherein conductive mineral assemblages span naturally occuring redox gradients and enable anaerobic microbes to access oxygen as an oxidant via extracellular electron transfer (or EET). To test this hypothesis, we ran a series of electrochemical laboratory experiments in which pyrite was used as an anode (in a vessel flushed with hydrothermal-like fluid). When placed in continuity with a carbon fiber cathode, pyrite was found to accept and conduct electrons from both abiotic and biological processes (microbial EET). Specifically, electrical current increased 4-fold (5 nA/m2 to 20 nA/m2) in response to inoculation with a slurry prepared from a hydrothermal vent sample. Inspection of the pyrite anode with SEM revealed ubiquitous coverage by microbes. DNA was extracted from the anodes and the inoculum, and was subjected to pyrosequencing to examine prokaryotic diversity. These data suggest that key microbial phylotypes were enriched upon the pyrite, implicating them in EET. In addition, we deployed an in situ experiment based on microbial fuel cell architecture with a graphite anode inserted into a vent wall coupled to a carbon fiber cathode outside the vent. We observed current production over the course of one year, implying microbial EET in situ. Via pyrosequencing, we observed that the microbial community on the anode was

  6. Deep-Sea Hydrothermal-Vent Sampler

    Science.gov (United States)

    Behar, Alberto E.; Venkateswaran, Kasthur; Matthews, Jaret B.

    2008-01-01

    An apparatus is being developed for sampling water for signs of microbial life in an ocean hydrothermal vent at a depth of as much as 6.5 km. Heretofore, evidence of microbial life in deep-sea hydrothermal vents has been elusive and difficult to validate. Because of the extreme conditions in these environments (high pressures and temperatures often in excess of 300 C), deep-sea hydrothermal- vent samplers must be robust. Because of the presumed low density of biomass of these environments, samplers must be capable of collecting water samples of significant volume. It is also essential to prevent contamination of samples by microbes entrained from surrounding waters. Prior to the development of the present apparatus, no sampling device was capable of satisfying these requirements. The apparatus (see figure) includes an intake equipped with a temperature probe, plus several other temperature probes located away from the intake. The readings from the temperature probes are utilized in conjunction with readings from flowmeters to determine the position of the intake relative to the hydrothermal plume and, thereby, to position the intake to sample directly from the plume. Because it is necessary to collect large samples of water in order to obtain sufficient microbial biomass but it is not practical to retain all the water from the samples, four filter arrays are used to concentrate the microbial biomass (which is assumed to consist of particles larger than 0.2 m) into smaller volumes. The apparatus can collect multiple samples per dive and is designed to process a total volume of 10 L of vent fluid, of which most passes through the filters, leaving a total possibly-microbe-containing sample volume of 200 mL remaining in filters. A rigid titanium nose at the intake is used for cooling the sample water before it enters a flexible inlet hose connected to a pump. As the water passes through the titanium nose, it must be cooled to a temperature that is above a mineral

  7. Evolutionary origins of hydrothermal vents metazoans

    Directory of Open Access Journals (Sweden)

    Samadi Sarah

    2015-01-01

    Full Text Available The discovery of the oases associated with hydrothermal vents in the deep sea, is probably the most fascinating discovery of oceanography of the last century. In this habitat, contrary to all expectations, a thriving development of unknown organisms was observed. At that time the knowledge about the deep sea organisms was very scarce and the accepted hypotheses about their evolutionary origins, their physiology or their ecology were very speculative. Almost forty years later, exploration of the deep-sea realm, but also of paleontological data together with the improvements in the phylogenetic methods, allowed the rejection of the hypothesis of an evolutionary history cut off the rest of the marine realm.

  8. Energetics of Amino Acid Synthesis in Alkaline Hydrothermal Environments

    Science.gov (United States)

    Kitadai, Norio

    2015-12-01

    Alkaline hydrothermal systems have received considerable attention as candidates for the origin and evolution of life on the primitive Earth. Nevertheless, sufficient information has not yet been obtained for the thermodynamic properties of amino acids, which are necessary components for life, at high temperatures and alkaline pH. These properties were estimated using experimental high-temperature volume and heat capacity data reported in the literature for several amino acids, together with correlation algorithms and the revised Helgeson-Kirkham-Flowers (HKF) equations of state. This approach enabled determination of a complete set of the standard molal thermodynamic data and the revised HKF parameters for the 20 protein amino acids in their zwitterionic and ionization states. The obtained dataset was then used to evaluate the energetics of amino acid syntheses from simple inorganic precursors (CO2, H2, NH3 and H2S) in a simulated alkaline hydrothermal system on the Hadean Earth. Results show that mixing between CO2-rich seawater and the H2-rich hydrothermal fluid can produce energetically favorable conditions for amino acid syntheses, particularly in the lower-temperature region of such systems. Together with data related to the pH and temperature dependences of the energetics of amino acid polymerizations presented in earlier reports, these results suggest the following. Hadean alkaline hydrothermal settings, where steep pH and temperature gradients may have existed between cool, slightly acidic Hadean ocean water and hot, alkaline hydrothermal fluids at the vent-ocean interface, may be energetically the most suitable environment for the synthesis and polymerization of amino acids.

  9. Dissolved gases in hydrothermal plumes from Artic vent fields

    OpenAIRE

    Stensland, Anne

    2013-01-01

    Recent discoveries of active vent fields on slow and ultraslow spreading ridges have revealed that hydrothermal activity is more common than previously assumed. In the Norwegian-Greenland Sea, two such vent fields are found; the Jan Mayen vent fields (71°N and 6°E) and Loki`s Castle (73°30′N and 8°E). Both these systems are located on the ultraslow spreading Mohns Ridge, but they differ profoundly in the geochemical fluid and gas composition. This study presen...

  10. Fluid and gas fluxes from the Logatchev hydrothermal vent area

    OpenAIRE

    Schmale, Oliver; Walter, Maren; Schneider von Deimling, Jens; Sültenfuß, Jürgen; Walker, Sharon; Rehder, Gregor; Keir, Robin

    2012-01-01

    The Logatchev hydrothermal field at 14°45′N on the MAR is characterized by gas plumes that are enriched in methane and helium compared to the oceanic background. We investigated CH4 concentration and δ13C together with δ3He in the water column of that region. These data and turbidity measurements indicate that apart from the known vent fields, another vent site exists northeast of the vent field Logatchev 1. The distribution of methane and 3He concentrations along two sections were used in co...

  11. Naked in toxic fluids: A nudibranch mollusc from hydrothermal vents

    Science.gov (United States)

    Valdés, Ángel; Bouchet, Philippe

    1998-01-01

    A new species of the nudibranch genus Dendronotus (Mollusca, Opisthobranchia) is reported from a hydrothermal vent at the Lucky Strike area, on the Mid-Atlantic Ridge. This is the first species of nudibranch recorded with certainty from a vent site. Other species of Dendronotus are distributed in temperate waters on the continental shelf of the northern hemisphere. Two factors that probably account for the occurrence of a nudibranch in this hydrothermal field are that the Lucky Strike area presents potential hydroid prey, and that nudibranchs apparently inhabit a lower activity area. It is hypothesized that the new species, which lacks eyes, is a permanent resident of vent fields on the Mid-Atlantic Ridge, but is probably not restricted to that environment.

  12. Discovery of new hydrothermal vent sites in Bransfield Strait, Antarctica

    Science.gov (United States)

    Klinkhammer, G. P.; Chin, C. S.; Keller, R. A.; Dählmann, A.; Sahling, H.; Sarthou, G.; Petersen, S.; Smith, F.; Wilson, C.

    2001-12-01

    We carried out a search for hydrothermal vents in the Central Basin of Bransfield Strait, Antarctica. The ZAPS (zero angle photon spectrometer) chemical sensor and instrument package (Oregon State University), OFOS (ocean-floor observation system) camera sled and TVG (TV-grab) (GEOMAR) were used to explore the water column and underlying seafloor. These operations were supplemented with a series of dredges. Hydrothermal plumes over Hook Ridge at the eastern end of the basin are confined to the E ridge crest and SE flank. The plumes are complex and sometimes contain two turbidity maxima one widespread feature centered at 1150 m and a smaller, more localized but broad maximum at 600-800 m. We traced the source of the shallower plume to a sunken crater near the ridge crest using sensors on the ZAPS instrument package. Subsequently two TV-grabs from the crater brought back hot, soupy sediment (42-49°C) overlain by hard, siliceous crusts and underlain by a thick layer of volcanic ash. We also recovered chimney fragments whose texture and mineralogy indicate venting temperatures in excess of 250°C. Native sulfur and Fe-sulfides occur in fractures and porous layers in sediment from throughout the area. Pore water data from the crater site are consistent with venting into a thin sediment layer and indicate phase separation of fluids beneath Hook Ridge. The source of the deeper plumes at Hook Ridge has yet to be located. We also explored a series of three parallel volcanic ridges west of Hook Ridge called Three Sisters. We detected water column anomalies indicative of venting with the ZAPS package and recovered hydrothermal barites and sulfides from Middle Sister. We spent considerable time photographing Middle Sister and Hook Ridge but did not identify classic vent fauna at either location. We either missed small areas with our photography or typical MOR vent fauna are absent at these sites.

  13. Differences in recovery between deep-sea hydrothermal vent and vent-proximate communities after a volcanic eruption

    NARCIS (Netherlands)

    Gollner, S.; Govenar, B.; Martinez Arbizu, P.; Mills, S.; Le Bris, N.; Weinbauer, M.; Shank, T.M.; Bright, M.

    2015-01-01

    Deep-sea hydrothermal vents and the surrounding basalt seafloor are subject to major natural disturbance events such as volcanic eruptions. In the near future, anthropogenic disturbance in the form of deep-sea mining could also significantly affect the faunal communities of hydrothermal vents. In th

  14. First hydrothermal active vent discovered on the Galapagos Microplate

    Science.gov (United States)

    Tao, C.; Li, H.; Wu, G.; Su, X.; Zhang, G.; Chinese DY115-21 Leg 3 Scientific Party

    2011-12-01

    The Galapagos Microplate (GM) lies on the western Gaplapagos Spreading Center (GSC), representing one of the classic Ridge-Ridge-Ridge (R-R-R) plate boundaries of the Nazca, Cocos, and Pacific plates. The presence of the 'black smoke' and hydrothermal vent community were firstly confirmed on the GSC. Lots of hydrothermal fields were discovered on the center and eastern GSC, while the western GSC has not been well investigated. During 17th Oct. to 9th Nov. 2009, the 3rd leg of Chinese DY115-21 cruise with R/V Dayangyihao has been launched along 2°N-5°S near equatorial East Pacific Rise (EPR). Two new hydrothermal fields were confirmed. One is named 'Precious Stone Mountain', which is the first hydrothermal field on the GM. The other is found at 101.47°W, 0.84°S EPR. The 'Precious Stone Mountain' hydrothermal field (at 101.49°W, 1.22°N) is located at an off-axial seamount on the southern GM boundary, with a depth from 1,450 to 1,700m. Hydrothermal fluids emitting from the fissures and hydrothermal fauna were captured by deep-tow video. Few mineral clasts of pyrite and chalcopyrite were separated from one sediment sample, but no sulfide chimney was found yet. Hydrothermal fauna such as alive mussels, crabs, shrimps, tubeworms, giant clams, as well as rock samples were collected by TV-Grab. The study of the seafloor classification with Simrad EM120 multi-beam echosounder has been conducted on the 'Precious Stone Mountain' hydrothermal field. The result indicates that seafloor materials around the hydrothermal field can be characterized into three types, such as the fresh lava, hydrothermal sediment, and altered rock.

  15. Geomicrobiology of deep-sea hydrothermal vents.

    Science.gov (United States)

    Jannasch, H W; Mottl, M J

    1985-08-23

    During the cycling of seawater through the earth's crust along the mid-ocean ridge system, geothermal energy is transferred into chemical energy in the form of reduced inorganic compounds. These compounds are derived from the reaction of seawater with crustal rocks at high temperatures and are emitted from warm (chemosynthesis. Bacteria grow most abundantly in the shallow crust where upwelling hot, reducing hydrothermal fluid mixes with downwelling cold, oxygenated seawater. The predominant production of biomass, however, is the result of symbiotic associations between chemolithotrophic bacteria and certain invertebrates, which have also been found as fossils in Cretaceous sulfide ores of ophiolite deposits. PMID:17841485

  16. Hydrothermal vents is Lake Tanganyika, East African Rift system

    Energy Technology Data Exchange (ETDEWEB)

    Tiercelin, J.J. [Universite de Bretagne Occidentale, Brest (France); Pflumio, C.; Castrec, M. [Universite Paris VI, Paris (France)] [and others

    1993-06-01

    Sublacustrine hydrothermal vents with associated massive sulfides were discovered during April 1987 at Pemba and Cape Banza on the Zaire side of the northern basin of Lake Tanganyika, East African Rift system. New investigations by a team of ten scuba divers during the multinational (France, Zaire, Germany, and Burundi) TANGANYDRO expedition (August-October 1991) found hydrothermal vents down to a depth of 46 m along north-trending active faults bounding the Tanganyika rift on the western side. Temperatures from 53 to 103 {degrees}C were measured in hydrothermal fluids and sediments. Veins of massive sulfides 1-10 cm thick (pyrite and marcasite banding) were found associated with vents at the Pemba site. At Cape Banza, active vents are characterized by 1-70-cm-high aragonite chimneys, and there are microcrystalline pyrite coatings on the walls of hydrothermal pipes. Hydrothermal fluid end members show distinctive compositions at the two sites. The Pemba end member is a NaHCO{sub 3}-enriched fluid similar to the NaHCO{sub 3} thermal fluids form lakes Magadi and Bogoria in the eastern branch of the rift. The Cape Banza end member is a solution enriched in NaCl. Such brines may have a deep-seated basement origin, as do the Uvinza NaCl brines on the eastern flank of the Tanganyika basin. Geothermometric calculations have yielded temperatures of fluid-rock interaction of 219 and 179 {degrees}C in the Pemba and Cape Banza systems, respectively. Abundant white or reddish-brown microbial colonies resembling Beggiatoa mats were found surrounding the active vents. Thermal fluid circulation is permitted by opening of cracks related to 130{degrees}N normal-dextral faults that intersect the north-south major rift trend. The sources of heat for such hydrothermal systems may relate to the existence of magmatic bodies under the rift, which is suggested by the isotopic composition of carbon dioxide released at Pemba and Cape Banza. 21 refs., 2 figs.

  17. Variation in the diets of hydrothermal vent gastropods

    Science.gov (United States)

    Govenar, Breea; Fisher, Charles R.; Shank, Timothy M.

    2015-11-01

    A prevailing paradigm of hydrothermal vent ecology is that primary consumers feed on chemoautotrophic bacteria. However, for the purposes of reconstructing vent food webs and for tracking energy flow from the generation of rock and fluid chemistry through primary/ secondary productivity and consumption to the overlying water column, it remains unclear which consumers feed on which bacteria. In paired analyses of carbon and nitrogen tissue stable isotope values with unique 16S rRNA sequences from the stomach contents, we determined that two species of gastropod grazers appear to feed on epsilon-proteobacteria, while two other species have more diverse diets, including one species that consumes alpha-proteobacteria, planctomycetes, and non-green sulfur bacteria. Different carbon fixation pathways used by epsilon- and alpha-proteobacteria may account for the variation in the carbon stable isotope values among the consumers. Furthermore, our results indicate that trophic specialization and niche partitioning may contribute to the distribution and abundance of vent-endemic gastropods and support the hypothesis that consumers in the warmer habitats commonly feed on epsilon-proteobacteria that use the rTCA cycle, while in the cooler habitats they feed on additional bacteria that use the CBB cycle. These results suggest that the phylogenetic and metabolic diversity of free-living bacteria may play an important and previously overlooked role in facilitating species coexistence among primary consumers at hydrothermal vents and other chemosynthesis-based ecosystems.

  18. Geomicrobiology of Deep-Sea Hydrothermal Vents

    Science.gov (United States)

    Jannasch, Holger W.; Mottl, Michael J.

    1985-08-01

    During the cycling of seawater through the earth's crust along the midocean ridge system, geothermal energy is transferred into chemical energy in the form of reduced inorganic compounds. These compounds are derived from the reaction of seawater with crustal rocks at high temperatures and are emitted from warm (methane-, hydrogen-, iron-, and manganese-oxidizing bacteria have also been found. Methanogenic, sulfur-respiring, and extremely thermophilic isolates carry out anaerobic chemosynthesis. Bacteria grow most abundantly in the shallow crust where upwelling hot, reducing hydrothermal fluid mixes with downwelling cold, oxygenated seawater. The predominant production of biomass, however, is the result of symbiotic associations between chemolithotrophic bacteria and certain invertebrates, which have also been found as fossils in Cretaceous sulfide ores of ophiolite deposits.

  19. Living with the Heat. Submarine Ring of Fire--Grades 5-6. Hydrothermal Vent Ecology.

    Science.gov (United States)

    National Oceanic and Atmospheric Administration (DOC), Rockville, MD.

    This activity is designed to teach about hydrothermal vent ecology. Students are expected to describe how hydrothermal vents are formed and characterize the physical conditions at these sites, explain chemosynthesis and contrast this process with photosynthesis, identify autotrophic bacteria as the basis for food webs in hydrothermal vent…

  20. Hydrothermal vent flow and turbulence measurements with acoustic scintillation instrumentation

    Science.gov (United States)

    di Iorio, D.; Xu, G.

    2009-12-01

    Acoustically derived measurements of hydrothermal vent flow and turbulence were obtained from the active black smoker Dante in the Main Endeavour vent field, using scintillation analysis from one-way transmissions. The scintillation transmitter and receiver array formed a 93 m acoustic path through the buoyant plume 20 m above the structure. The acoustic path was parallel to the valley sidewall where the M2 tidal currents are approximately aligned along ridge due to topographic steering by the valley walls and hence most of the plume displacement is expected to occur along the acoustic path. On one deployment, data were collected for 6.5 weeks and vertical velocities range from 0.1 to 0.2 m/s showing a strong dependence on the spring/neap tidal cycle. The refractive index fluctuations which can be paramaterized in terms of the root-mean-square temperature fluctuations also shows a strong tidal modulation during spring tide.

  1. Hydrothermal vents and methane seeps: Rethinking the sphere of influence

    Science.gov (United States)

    Levin, Lisa A.; Baco, Amy; Bowden, David; Colaco, Ana; Cordes, Erik E.; Cunha, Marina; Demopoulos, Amanda; Gobin, Judith; Grupe, Ben; Le, Jennifer; Metaxas, Anna; Netburn, Amanda; Rouse, Greg; Thurber, Andrew; Tunnicliffe, Verena; Van Dover, Cindy L.; Vanreusel, Ann; Watling, Les

    2016-01-01

    Although initially viewed as oases within a barren deep ocean, hydrothermal vent and methane seep communities are now recognized to interact with surrounding ecosystems on the sea floor and in the water column, and to affect global geochemical cycles. The importance of understanding these interactions is growing as the potential rises for disturbance from oil and gas extraction, seabed mining and bottom trawling. Here we synthesize current knowledge of the nature, extent and time and space scales of vent and seep interactions with background systems. We document an expanded footprint beyond the site of local venting or seepage with respect to elemental cycling and energy flux, habitat use, trophic interactions, and connectivity. Heat and energy are released, global biogeochemical and elemental cycles are modified, and particulates are transported widely in plumes. Hard and biotic substrates produced at vents and seeps are used by “benthic background” fauna for attachment substrata, shelter, and access to food via grazing or through position in the current, while particulates and fluid fluxes modify planktonic microbial communities. Chemosynthetic production provides nutrition to a host of benthic and planktonic heterotrophic background species through multiple horizontal and vertical transfer pathways assisted by flow, gamete release, animal movements, and succession, but these pathways remain poorly known. Shared species, genera and families indicate that ecological and evolutionary connectivity exists among vents, seeps, organic falls and background communities in the deep sea; the genetic linkages with inactive vents and seeps and background assemblages however, are practically unstudied. The waning of venting or seepage activity generates major transitions in space and time that create links to surrounding ecosystems, often with identifiable ecotones or successional stages. The nature of all these interactions is dependent on water depth, as well as

  2. Hydrothermal Vents and Methane Seeps: Rethinking the Sphere of Influence

    Directory of Open Access Journals (Sweden)

    Lisa Ann Levin

    2016-05-01

    Full Text Available Although initially viewed as oases within a barren deep ocean, hydrothermal vent and methane seep communities are now recognized to interact with surrounding ecosystems on the sea floor and in the water column, and to affect global geochemical cycles. The importance of understanding these interactions is growing as the potential rises for disturbance from oil and gas extraction, seabed mining and bottom trawling. Here we synthesize current knowledge of the nature, extent and time and space scales of vent and seep interactions with background systems. We document an expanded footprint beyond the site of local venting or seepage with respect to elemental cycling and energy flux, habitat use, trophic interactions, and connectivity. Heat and energy are released, global biogeochemical and elemental cycles are modified, and particulates are transported widely in plumes. Hard and biotic substrates produced at vents and seeps are used by benthic background fauna for attachment substrata, shelter, and access to food via grazing or through position in the current, while particulates and fluid fluxes modify planktonic microbial communities. Chemosynthetic production provides nutrition to a host of benthic and planktonic heterotrophic background species through multiple horizontal and vertical transfer pathways assisted by flow, gamete release, animal movements, and succession, but these pathways remain poorly known. Shared species, genera and families indicate that ecological and evolutionary connectivity exists among vents, seeps, organic falls and background communities in the deep sea; the genetic linkages with inactive vents and seeps and background assemblages however, are practically unstudied. The waning of venting or seepage activity generates major transitions in space and time that create links to surrounding ecosystems, often with identifiable ecotones or successional stages. The nature of all these interactions is dependent on water depth, as

  3. Euryhaline Halophilic Microorganisms From the Suiyo Seamount Hydrothermal Vents.

    Science.gov (United States)

    Okamoto, T.; Kimura, H.; Maruyama, A.; Naganuma, T.

    2002-12-01

    The euryhaline halophilic microorganisms grow in a wide salinity range from 15% NaCl or to even saturation (about 30% NaCl). A number of euryhaline halophiles have been found in a wide range of habitats from oceanic and terrestrial regimes, from deep-sea vents and seeps, and from Antarctic sea ice and terrains. We have isolated the euryhaline strains independently from a Mid-Atlantic Ridge vent fluids and Antarctic terrains are closely related species of the genus Halomonas. Some euryhaline halophiles maintain intracellular osmotic balance by controlling the concentration of compatible solute such as ectoine. This compatible solute not only stabilizes the proteins from denaturation caused by high salt concentration but also serves as a protectant against stresses such as heating, freezing and drying. The sub-seafloor structure of a hydrothermal vent is highly complicated with mosaic heterogeneity of physicochemical parameters such as temperature and salinity. This premise led us to the hypothesis that some euryhaline halophiles including Halomonas species well adapt to a wide salinity-ranged habitat in the sub-vent. To test this hypothesis, isolation and characterization of euryhaline halophiles from the Suiyo Seamount hydrothermal vents were conducted the drill-cored rock samples from the sites APSK-02, 03, and 07 and the filter-trapped fluid particle samples from the sites APSK-01 and 05 were used. For initial cultivation, a heterotrophic bacterial medium of 15% NaCl was used. The samples was added to the medium and incubated under both aerobic and anaerobic conditions at room temperature. A total of 5 euryhaline halophilic strains were obtained and phylogenetically characterized: two strains (both related to Marinobacter) from APSK-02 core section 2; one strain (related to H. meridiana) from APSK-07 core section 3; and two strains (related to H. meridiana and H. variabilis) from APSK-01 trapped particles. In addition, some thermophilic halophiles that grow at 20

  4. Mackinawite and greigite in ancient alkaline hydrothermal chimneys: Identifying potential key catalysts for emergent life

    Science.gov (United States)

    White, Lauren M.; Bhartia, Rohit; Stucky, Galen D.; Kanik, Isik; Russell, Michael J.

    2015-11-01

    One model for the emergence of life posits that ancient, low temperature, submarine alkaline hydrothermal vents, partly composed of iron-sulfides, were capable of catalyzing the synthesis of prebiotic organic molecules from CO2, H2 and CH4. Specifically, hydrothermal mackinawite (FeIIS) and greigite (FeIIFeIII2S4) have been highlighted in previous studies as analogs of the active centers of hydrogenase, ferredoxin, acetyl coenzyme-A synthase and carbon monoxide dehydrogenase featured in the biochemistry of certain autotrophic prokaryotes that occupy the base of the evolutionary tree. Despite the proposed importance of iron sulfide minerals and clusters in the synthesis of abiotic organic molecules, the mechanisms for the formation of these sulfides from solution and their preservation under the anoxic and low temperature (below 100 °C) conditions expected in off-axis submarine alkaline vent systems is not well understood (Bourdoiseau et al., 2011; Rickard and Luther, 2007). To rectify this, single hydrothermal chimneys were precipitated using a unique apparatus to simulate growth at hydrothermal vents of moderate temperature under supposed Hadean ocean-bottom conditions. Iron sulfide phases were observed through Raman spectroscopy at growth temperatures ranging from 40° to 80 °C. Fe(III)-containing mackinawite is confirmed to be present with mackinawite and greigite, supporting an FeIII-mackinawite intermediate mechanism for the transformation of mackinawite to greigite below 100 °C. Raman spectroscopy of the chimneys revealed a maximum yield of greigite at 75 °C. These results suggest abiotic production of catalytically active mackinawite and greigite are possible under early Earth hydrothermal conditions as well as on other wet, rocky worlds geochemically similar to the Earth.

  5. Hydrothermal vent fields and chemosynthetic biota on the world's deepest seafloor spreading centre.

    OpenAIRE

    Connelly, D. P.; Copley, J.T.; Murton, B.J.; Stansfield, K.; Tyler, P. A.; German, C.R.; Van Dover, C.L.; Amon, D.; Furlong, M.; Grindlay, N.; Hayman, N; Huhnerbach, V.; Judge, M; Le Bas, T; McPhail, S

    2012-01-01

    The Mid-Cayman spreading centre is an ultraslow-spreading ridge in the Caribbean Sea. Its extreme depth and geographic isolation from other mid-ocean ridges offer insights into the effects of pressure on hydrothermal venting, and the biogeography of vent fauna. Here we report the discovery of two hydrothermal vent fields on the Mid-Cayman spreading centre. The Von Damm Vent Field is located on the upper slopes of an oceanic core complex at a depth of 2,300 m. High-temperature venting in this ...

  6. Hydrothermal vent fields and chemosynthetic biota on the world's deepest seafloor spreading centre

    OpenAIRE

    Connelly, Douglas P.; Copley, Jaonathan T.; Murton, Bramley J.; Stansfield, Kate; Tyler, Paul A.; German, Cristopher R.; Van Dover, Cindy L.; Amon, Diva; Furlong, Maaten; Grindlay, Nancy; Hayman, Nicholas; Hühnerbach, Veit; Judge, Maria; Le Bas, Tim; McPhail, Stephen

    2012-01-01

    The Mid-Cayman spreading centre is an ultraslow-spreading ridge in the Caribbean Sea. Its extreme depth and geographic isolation from other mid-ocean ridges offer insights into the effects of pressure on hydrothermal venting, and the biogeography of vent fauna. Here we report the discovery of two hydrothermal vent fields on the Mid-Cayman spreading centre. The Von Damm Vent Field is located on the upper slopes of an oceanic core complex at a depth of 2,300 m. High-temperature venting in this ...

  7. Vacuolate-attached filaments: highly productive Ridgeia piscesae epibionts at the Juan de Fuca hydrothermal vents

    OpenAIRE

    Kalanetra, Karen M.; Nelson, Douglas C.

    2010-01-01

    Vacuolate sulfur bacteria with high morphological similarity to vacuolate-attached filaments previously described from shallow hydrothermal vents (White Point, CA) were found at deep-sea hydrothermal vents. These filamentous bacteria grow in dense mats that cover surfaces and potentially provide a significant source of organic carbon where they occur. Vacuolate-attached filaments were collected near vents at the Clam Bed site of the Endeavour Segment of the Juan de Fuca Ridge and from the sed...

  8. Endemic hydrothermal vent species identified in the open ocean seed bank.

    Science.gov (United States)

    Gonnella, Giorgio; Böhnke, Stefanie; Indenbirken, Daniela; Garbe-Schönberg, Dieter; Seifert, Richard; Mertens, Christian; Kurtz, Stefan; Perner, Mirjam

    2016-01-01

    Hydrothermal vent systems host microbial communities among which several microorganisms have been considered endemic to this type of habitat. It is still unclear how these organisms colonize geographically distant hydrothermal environments. Based on 16S rRNA gene sequences, we compare the bacterial communities of sixteen Atlantic hydrothermal vent samples with our own and publicly available global open ocean samples. Analysing sequences obtained from 63 million 16S rRNA genes, the genera we could identify in the open ocean waters contained 99.9% of the vent reads. This suggests that previously observed vent exclusiveness is, in most cases, probably an artefact of lower sequencing depth. These findings are a further step towards elucidating the role of the open ocean as a seed bank. They can explain the predicament of how species expected to be endemic to vent systems are able to colonize geographically distant hydrothermal habitats and contribute to our understanding of whether 'everything is really everywhere'. PMID:27573109

  9. Anaerobic Respiration on Tellurate and Other Metalloids in Bacteria from Hydrothermal Vent Fields in the Eastern Pacific Ocean

    OpenAIRE

    Csotonyi, Julius T.; Stackebrandt, Erko; Yurkov, Vladimir

    2006-01-01

    This paper reports the discovery of anaerobic respiration on tellurate by bacteria isolated from deep ocean (1,543 to 1,791 m) hydrothermal vent worms. The first evidence for selenite- and vanadate-respiring bacteria from deep ocean hydrothermal vents is also presented. Enumeration of the anaerobic metal(loid)-resistant microbial community associated with hydrothermal vent animals indicates that a greater proportion of the bacterial community associated with certain vent fauna resists and red...

  10. Heat flux measured acoustically at Grotto Vent, a hydrothermal vent cluster on the Endeavour Segment, Juan de Fuca Ridge

    Science.gov (United States)

    Xu, G.; Jackson, D. R.; Bemis, K. G.; Rona, P. A.

    2013-12-01

    Over the past several decades, quantifying the heat output has been a unanimous focus of studies at hydrothermal vent fields discovered around the global ocean. Despite their importance, direct measurements of hydrothermal heat flux are very limited due to the remoteness of most vent sites and the complexity of hydrothermal venting. Moreover, almost all the heat flux measurements made to date are snapshots and provide little information on the temporal variation that is expected from the dynamic nature of a hydrothermal system. The Cabled Observatory Vent Imaging Sonar (COVIS, https://sites.google.com/a/uw.edu/covis/) is currently connected to the Endeavour node of the NEPTUNE Canada observatory network (http://www.neptunecanada.ca) to monitor the hydrothermal plumes issuing from a vent cluster (Grotto) on the Endeavour Segment of the Juan de Fuca Ridge. COVIS is acquiring a long-term (20-months to date) time series of the vertical flow rate and volume flux of the hydrothermal plume above Grotto through the Doppler analysis of the acoustic backscatter data (Xu et al., 2013). We then estimate the plume heat flux from vertical flow rate and volume flux using our newly developed inverse method. In this presentation, we will briefly summarize the derivation of the inverse method and present the heat-flux time series obtained consequently with uncertainty quantification. In addition, we compare our heat-flux estimates with the one estimated from the plume in-situ temperatures measured using a Remotely Operative Vehicle (ROV) in 2012. Such comparison sheds light on the uncertainty of our heat flux estimation. Xu, G., Jackson, D., Bemis, K., and Rona, P., 2013, Observations of the volume flux of a seafloor hydrothermal plume using an acoustic imaging sonar, Geochemistry, Geophysics Geosystems, 2013 (in press).

  11. Arsenic speciation in shrimp and mussel from the Mid-Atlantic hydrothermal vents

    DEFF Research Database (Denmark)

    Larsen, Erik Huusfeldt; Quetel, C. R.; Munoz, R.; FialaMedioni, A.; Donard, O. F. X.

    Specimens of shrimp (Rimicaris exoculata) and mussel (Bathymodiolus puteoserpentis) were collected 3500 m below the ocean surface at the hydrothermal vents of the mid-Atlantic Ridge (TAG and Snake Pit sites, respectively). Arsenic, a potentially toxic element, is among the substances emitted by the...... hydrothermal vents. The hydrothermal vent shrimp, which are known to be a primary consumer of the primary producing chemolithoautotrophic bacteria, contained arsenic at 13 mu g g(-1) almost exclusively as arsenobetaine (AsB). Arsenic was present in the soft:issues of the mussel at 40 mu g g(-1) and the major...... of arsenic species found in the shrimp and mussel species in the deep-sea is similar to that found in their counterparts from the ocean surface. It is concluded that the autotrophic bacteria of the hydrothermal vent ecosystem and the symbiotic bacteria harboured in the mussel species are responsible...

  12. Arsenic speciation in shrimp and mussel from the Mid-Atlantic hydrothermal vents

    DEFF Research Database (Denmark)

    Larsen, Erik Huusfeldt; Quetel, C. R.; Munoz, R.;

    1997-01-01

    Specimens of shrimp (Rimicaris exoculata) and mussel (Bathymodiolus puteoserpentis) were collected 3500 m below the ocean surface at the hydrothermal vents of the mid-Atlantic Ridge (TAG and Snake Pit sites, respectively). Arsenic, a potentially toxic element, is among the substances emitted by the...... hydrothermal vents. The hydrothermal vent shrimp, which are known to be a primary consumer of the primary producing chemolithoautotrophic bacteria, contained arsenic at 13 mu g g(-1) almost exclusively as arsenobetaine (AsB). Arsenic was present in the soft:issues of the mussel at 40 mu g g(-1) and the major...... of arsenic species found in the shrimp and mussel species in the deep-sea is similar to that found in their counterparts from the ocean surface. It is concluded that the autotrophic bacteria of the hydrothermal vent ecosystem and the symbiotic bacteria harboured in the mussel species are responsible...

  13. Hydrogen-limited growth of hyperthermophilic methanogens at deep-sea hydrothermal vents

    OpenAIRE

    Ver Eecke, Helene C.; David A Butterfield; Julie A. Huber; Lilley, Marvin D.; Olson, Eric J.; Roe, Kevin K.; Evans, Leigh J.; Merkel, Alexandr Y.; Cantin, Holly V.; Holden, James F.

    2012-01-01

    Microbial productivity at hydrothermal vents is among the highest found anywhere in the deep ocean, but constraints on microbial growth and metabolism at vents are lacking. We used a combination of cultivation, molecular, and geochemical tools to verify pure culture H2 threshold measurements for hyperthermophilic methanogenesis in low-temperature hydrothermal fluids from Axial Volcano and Endeavour Segment in the northeastern Pacific Ocean. Two Methanocaldococcus strains from Axial and Methan...

  14. Hydrothermal vents in Lake Tanganyika harbor spore-forming thermophiles with extremely rapid growth

    DEFF Research Database (Denmark)

    Elsgaard, Lars; Prieur, Daniel

    2010-01-01

    A thermophilic anaerobic bacterium was isolated from a sublacustrine hydrothermal vent site in Lake Tanganyika (East Africa) with recorded fluid temperatures of 66–103 °C and pH values of 7.7–8.9. The bacterium (strain TR10) was rod-shaped, about 1 by 5 μm in size, and readily formed distal...... organism to exploit the temporarily fluctuating growth conditions in the hydrothermal vent environments of Lake Tanganyika...

  15. Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific.

    OpenAIRE

    SayakaMino; HirokoMaikita; TomohiroToki; ShingoKato; HiromiWatanabe; ShigeakiKojima

    2013-01-01

    Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephonella are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure ...

  16. Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific

    OpenAIRE

    Mino, Sayaka; Makita, Hiroko; Toki, Tomohiro; Miyazaki, Junichi; Kato, Shingo; Watanabe, Hiromi; Imachi, Hiroyuki; Watsuji, Tomo-o; Nunoura, Takuro; Kojima, Shigeaki; Sawabe, Tomoo; Takai, Ken; Nakagawa, Satoshi

    2013-01-01

    Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephonella are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure ...

  17. Chemolithotrophic Sulfur-Oxidizing Bacteria from the Galapagos Rift Hydrothermal Vents

    OpenAIRE

    Ruby, E. G.; Wirsen, C.O. (C.); Jannasch, H W

    1981-01-01

    Three distinct physiological types of sulfur-oxidizing bacteria were enriched and isolated from samples collected at several deep-sea hydrothermal vents (2,550 m) of the Galapagos Rift ocean floor spreading center. Twelve strains of the obligately chemolithotrophic genus Thiomicrospira were obtained from venting water and from microbial mats covering surfaces in the immediate vicinity of the vents. From these and other sources two types of obligately heterotrophic sulfur oxidizers were repeat...

  18. Macrofauna of shallow hydrothermal vents on the Arctic Mid-Ocean Ridge at 71N

    Science.gov (United States)

    Schander, C.; Rapp, H. T.; Pedersen, R. B.

    2007-12-01

    Deep-sea hydrothermal vents are usually associated with a highly specialized fauna and since their discovery in 1977, more than 400 species of animals have been described. Specialized vent fauna includes various animal phyla, but the most conspicuous and well known are annelids, mollusks and crustaceans. We have investigated the fauna collected around newly discovered hydrothermal vents on the Mohns Ridge north of Jan Mayen. The venting fields are located at 71°N and the venting takes place within two main areas separated by 5 km. The shallowest vent area is at 500-550 m water depth and is located at the base of a normal fault. This vent field stretches approximately 1 km along the strike of the fault, and it is composed of 10-20 major vent sites each with multiple chimney constructions discharging up to 260°C hot fluids. A large area of diffuse, low- temperature venting occurs in the area surrounding the high-temperature field. Here, partly microbial mediated iron-oxide-hydroxide deposits are abundant. The hydrothermal vent sites do not show any high abundance of specialized hydrothermal vent fauna. Single groups (i.e. Porifera and Mollusca) have a few representatives but groups otherwise common in hydrothermal vent areas (e.g. vestimentifera, Alvinellid worms, mussels, clams, galathaeid and brachyuran crabs) are absent. Up until now slightly more than 200 species have been identified from the vent area. The macrofauna found in the vent area is, with few exceptions, an assortment of bathyal species known in the area. One endemic, yet undescribed, species of mollusc has been found so far, an gastropod related to Alvania incognita Warén, 1996 and A. angularis Warén, 1996 (Rissoidae), two species originally described from pieces of sunken wood north and south of Iceland. It is by far the most numerous mollusc species at the vents and was found on smokers, in the bacterial mats, and on the ferric deposits. A single specimen of an undescribed tanaidacean has also

  19. Hydrothermal vent fields and chemosynthetic biota on the world's deepest seafloor spreading centre.

    Science.gov (United States)

    Connelly, Douglas P; Copley, Jonathan T; Murton, Bramley J; Stansfield, Kate; Tyler, Paul A; German, Christopher R; Van Dover, Cindy L; Amon, Diva; Furlong, Maaten; Grindlay, Nancy; Hayman, Nicholas; Hühnerbach, Veit; Judge, Maria; Le Bas, Tim; McPhail, Stephen; Meier, Alexandra; Nakamura, Ko-Ichi; Nye, Verity; Pebody, Miles; Pedersen, Rolf B; Plouviez, Sophie; Sands, Carla; Searle, Roger C; Stevenson, Peter; Taws, Sarah; Wilcox, Sally

    2012-01-01

    The Mid-Cayman spreading centre is an ultraslow-spreading ridge in the Caribbean Sea. Its extreme depth and geographic isolation from other mid-ocean ridges offer insights into the effects of pressure on hydrothermal venting, and the biogeography of vent fauna. Here we report the discovery of two hydrothermal vent fields on the Mid-Cayman spreading centre. The Von Damm Vent Field is located on the upper slopes of an oceanic core complex at a depth of 2,300 m. High-temperature venting in this off-axis setting suggests that the global incidence of vent fields may be underestimated. At a depth of 4,960 m on the Mid-Cayman spreading centre axis, the Beebe Vent Field emits copper-enriched fluids and a buoyant plume that rises 1,100 m, consistent with >400 °C venting from the world's deepest known hydrothermal system. At both sites, a new morphospecies of alvinocaridid shrimp dominates faunal assemblages, which exhibit similarities to those of Mid-Atlantic vents. PMID:22233630

  20. Deep-sea hydrothermal vents: potential hot spots for natural products discovery?

    Science.gov (United States)

    Thornburg, Christopher C; Zabriskie, T Mark; McPhail, Kerry L

    2010-03-26

    Deep-sea hydrothermal vents are among the most extreme and dynamic environments on Earth. However, islands of highly dense and biologically diverse communities exist in the immediate vicinity of hydrothermal vent flows, in stark contrast to the surrounding bare seafloor. These communities comprise organisms with distinct metabolisms based on chemosynthesis and growth rates comparable to those from shallow water tropical environments, which have been rich sources of biologically active natural products. The geological setting and geochemical nature of deep-sea vents that impact the biogeography of vent organisms, chemosynthesis, and the known biological and metabolic diversity of Eukarya, Bacteria, and Archaea, including the handful of natural products isolated to date from deep-sea vent organisms, are considered here in an assessment of deep-sea hydrothermal vents as potential hot spots for natural products investigations. Of critical importance too are the logistics of collecting deep vent organisms, opportunities for re-collection considering the stability and longevity of vent sites, and the ability to culture natural product-producing deep vent organisms in the laboratory. New cost-effective technologies in deep-sea research and more advanced molecular techniques aimed at screening a more inclusive genetic assembly are poised to accelerate natural product discoveries from these microbial diversity hot spots. PMID:20099811

  1. Spatial distribution pattern of seafloor hydrothermal vents to the southeastern Kueishan Tao offshore Taiwan Island

    Institute of Scientific and Technical Information of China (English)

    HAN Chenhua; YE Ying; PAN Yiwen; QIN Huawei; WU Guanghai; CHEN Chen-Tung Arthur

    2014-01-01

    Investigations of the diffusion activities both within and outside the seafloor hydrothermal vents, as well as related mineral genesis, have been one of the key focuses of ocean biogeochemistry studies. Many hy-drothermal vents are distributed close to the southern Okinawa Trough on the less-than-30-m deep shal-low seafloor off Kueishan Tao, northeast of Taiwan Island. Investigations of temperature, pH and Eh at four depths of hydrothermal plume were carried out near Kueishan Tao at the white (24.83°N, 121.96°E) and yellow (24.83°N, 121.96°E) vents. An 87 h of temperature time series observation-undertaken near the white vent showed that tide is the main factor affecting the background environment. Based on the observed data, 3-dimensional sliced diffusion fields were obtained and analyzed. It was concluded that the plume diffused mainly from north to south due to ebb tide. The yellow vent’s plume could effect as far as the white vent surface. From the temperature diffusion field, the vortices of the plume were observed. The Eh negative abnormality was a better indicator to search for hydrothermal plumes and locate hydrothermal vents than high temperature and low pH abnormalities.

  2. Energy Transfer Through Food Webs at Hydrothermal Vents: Linking the Lithosphere to the Biosphere

    OpenAIRE

    Breea Govenar

    2012-01-01

    Tectonic and volcanic processes that drive hydrothermal fluid flow and influence its chemistry also regulate the transfer of energy to hydrothermal vent ecosystems. Chemoautotrophic bacteria use the chemical energy generated by mixing the reduced chemicals in hydrothermal fluids with deep-ocean ambient seawater to fix inorganic carbon and produce biomass. These and other microbes, or their products, are then consumed by other organisms, which are subsequently consumed by other organisms. The ...

  3. Molecular Ecology of Free-Living Chemoautotrophic Microbial Communities at a Shallow-sea Hydrothermal Vent

    OpenAIRE

    Huang, Chia-I

    2012-01-01

    Deep-sea hydrothermal systems are unique habitats for microbial life with primary production based on chemosynthesis. They are considered to be windows to the subsurface biosphere. Their far more accessible shallow-sea counterparts are valuable targets to study the effects of hydrothermal activity on geology, seawater chemistry and microorganisms. Such an area of shallow-sea hydrothermal venting is observed approximately 2.5 km east off Panarea Island (Sicily, Italy). This system is character...

  4. Hydrothermal Venting in the Southern Most Portion of the Mariana Backarc Spreading Center at 12.57 Degrees N

    Science.gov (United States)

    Wheat, C. G.; Fryer, P.; Hulme, S.; Becker, N.; Curtis, A.; Moyer, C.

    2003-12-01

    The southernmost portion of the Mariana Trough is a complex zone of deformation and magmatism. The centerpiece of the trough is the Malaguana-Gadao Spreading Center Ridge, which has a morphology that is inflated relative to all of the other spreading segments in the Mariana Backarc Basin. This inflated morphology is similar to that of the EPR and is consistent with a high rate of eruption. Samples of lavas from the spreading center are island arc tholeiites, which typically contain higher concentrations of CO2 relative to MORBs. In February 2003 the NOAA VENTS program conducted two CTD tow-yos in this area, revealing a hydrothermal plume consistent with the presence of a high temperature hydrothermal site. In May 2003 we used the ROV Jason II to survey a hydrothermal site at 12 degrees 57.214'N, 143 degrees 37.147'E in a water depth of ~2860 m. Here we collected vent fluids, sulfides, basalt, microbial mats, and macrofauna. Two styles of venting were identified within an 80 m by 70 m area. Higher temperature venting was located in cracks among extinct mounds that were 2-5 m high and wide and are nearly monomineralic (sphalerite) in composition. The exterior is soft and vuggy in contrast to the interior that has crystalline layers with bladed habit lining the orifices. Maximum temperatures of 248 degrees C were recorded 30 cm into these cracks, which were covered with abundant large provannid snails, Alvinoconcha Hessleri, and crabs. In contrast, lower temperature diffuse venting (77 degrees C) emanated from mounds that have a porous structure that is composed of amorphous iron oxide and opal. These mounds are covered with microbial mats, which exhibit a high degree of biomass as detected by Cyto-13 nucleic acid epifluorescent staining. Filament and amorphous particulate morphotypes are observed in association with microbial cells, but sheaths have not been detected. These mats have a morphology that is similar to that of neutrophilic Fe-oxidizing bacteria. Fluid

  5. Reproductive biology, sexual dimorphism, and population structure of the deep sea hydrothermal vent scale-worm, Branchiplynoee seepensis (Polychaeta : Polynoidae)

    OpenAIRE

    Jollivet, D.; Empis, A; Baker, Mc; Hourdez, S; Comtet, Thierry; Jouin-toulmond, C; Desbruyeres, Daniel; Tyler, PA

    2000-01-01

    The polychaete family Polynoidae (scale-worms) is well-represented at deep sea hydrothermal vents. Most species are free-living in a wide range of habitats: from high-temperature hydrothermal `chimney' walls to diffuse venting areas. Conversely, species of the genus Branchipolynoe live inside the mantle cavity of vent and seep mytilids. Specimens, morphologically close to Branchipolynoe seepensis, have been reported from all the known vent areas on the Mid-Atlantic Ridge (MAR), with varying i...

  6. Community Structure of Macrobiota and Environmental Parameters in Shallow Water Hydrothermal Vents off Kueishan Island, Taiwan.

    Science.gov (United States)

    Chan, Benny Kwok Kan; Wang, Teng-Wei; Chen, Pin-Chen; Lin, Chia-Wei; Chan, Tin-Yam; Tsang, Ling Ming

    2016-01-01

    Hydrothermal vents represent a unique habitat in the marine ecosystem characterized with high water temperature and toxic acidic chemistry. Vents are distributed at depths ranging from a few meters to several thousand meters. The biological communities of shallow-water vents have, however, been insufficiently studied in most biogeographic areas. We attempted to characterize the macrofauna and macroflora community inhabiting the shallow-water vents off Kueishan Island, Taiwan, to identify the main abiotic factors shaping the community structure and the species distribution. We determined that positively buoyant vent fluid exhibits a more pronounced negative impact to species on the surface water than on the bottom layer. Species richness increased with horizontal distance from the vent, and continuing for a distance of 2000 m, indicating that the vent fluid may exert a negative impact over several kilometers. The community structure off Kueishan Island displayed numerous transitions along the horizontal gradient, which were broadly congruent with changes in environmental conditions. Combination of variation in Ca2+, Cl-, temperature, pH and depth were revealed to show the strongest correlation with the change in benthic community structure, suggesting multiple factors of vent fluid were influencing the associated fauna. Only the vent crabs of Kueishan Island may have an obligated relationship with vents and inhabit the vent mouths because other fauna found nearby are opportunistic taxa that are more tolerant to acidic and toxic environments. PMID:26849440

  7. Community Structure of Macrobiota and Environmental Parameters in Shallow Water Hydrothermal Vents off Kueishan Island, Taiwan.

    Directory of Open Access Journals (Sweden)

    Benny Kwok Kan Chan

    Full Text Available Hydrothermal vents represent a unique habitat in the marine ecosystem characterized with high water temperature and toxic acidic chemistry. Vents are distributed at depths ranging from a few meters to several thousand meters. The biological communities of shallow-water vents have, however, been insufficiently studied in most biogeographic areas. We attempted to characterize the macrofauna and macroflora community inhabiting the shallow-water vents off Kueishan Island, Taiwan, to identify the main abiotic factors shaping the community structure and the species distribution. We determined that positively buoyant vent fluid exhibits a more pronounced negative impact to species on the surface water than on the bottom layer. Species richness increased with horizontal distance from the vent, and continuing for a distance of 2000 m, indicating that the vent fluid may exert a negative impact over several kilometers. The community structure off Kueishan Island displayed numerous transitions along the horizontal gradient, which were broadly congruent with changes in environmental conditions. Combination of variation in Ca2+, Cl-, temperature, pH and depth were revealed to show the strongest correlation with the change in benthic community structure, suggesting multiple factors of vent fluid were influencing the associated fauna. Only the vent crabs of Kueishan Island may have an obligated relationship with vents and inhabit the vent mouths because other fauna found nearby are opportunistic taxa that are more tolerant to acidic and toxic environments.

  8. Shallow Water Hydrothermal Vents in the Gulf of California: Natural Laboratories for Multidisciplinary Research

    Science.gov (United States)

    Forrest, M.; Hilton, D. R.; Price, R. E.; Kulongoski, J. T.

    2015-12-01

    Modern and fossil examples of shallow water submarine hydrothermal vents occur throughout the Gulf of California. These sites offer important information about the processes involved in the extensional tectonics that created the Gulf of California and continue to shape the region to this day. Due to their accessibility, shallow water marine hydrothermal vents are far easier to access and study than their deeper analogs, and these settings can provide natural laboratories to study biogeochemical processes. Certain biogeochemical and biomineralizing processes occurring at shallow vents are very similar to those observed around deep-sea hydrothermal vents. In some cases, authigenic carbonates form around shallow vents. However, the hydrothermal precipitates are generally composed of Fe-oxyhydroxides, Mn-oxides, opal, calcite, pyrite and cinnabar, and their textural and morphological characteristics suggest microbial mediation for mineral deposition. Modern shallow-water hydrothermal vents also support complex biotic communities, characterized by the coexistence of chemosynthetic and photosynthetic organisms. These shallow vents are highly productive and provide valuable resources to local fishermen. Extant shallow water hydrothermal activity has been studied in Bahía Concepción, San Felipe, Punta Estrella, El Coloradito, Puertecitos, and around the Islas Encantadas. Discrete streams of gas bubbles are often discharged along with hot liquids at shallow water vents. The vent liquids generally exhibit lower salinities than seawater, and their isotopic compositions indicate that they contain meteoric water mixed with seawater. The composition of the shallow vent gas is primarily made up of CO2, but may also be enriched in N2, H2S, CH4, and other higher hydrocarbons. The geochemistry of these gases can be informative in determining the sources and processes involved in their generation. In particular, 3He/4He ratios may provide valuable information about the origin of

  9. Community Structure Comparisons of Hydrothermal Vent Microbial Mats Along the Mariana Arc and Back-arc

    Science.gov (United States)

    Hager, K. W.; Fullerton, H.; Moyer, C. L.

    2015-12-01

    Hydrothermal vents along the Mariana Arc and back-arc represent a hotspot of microbial diversity that has not yet been fully recognized. The Mariana Arc and back-arc contain hydrothermal vents with varied vent effluent chemistry and temperature, which translates to diverse community composition. We have focused on iron-rich sites where the dominant primary producers are iron oxidizing bacteria. Because microbes from these environments have proven elusive in culturing efforts, we performed culture independent analysis among different microbial communities found at these hydrothermal vents. Terminal-restriction fragment length polymorphism (T-RFLP) and Illumina sequencing of small subunit ribosomal gene amplicons were used to characterize community members and identify samples for shotgun metagenomics. Used in combination, these methods will better elucidate the composition and characteristics of the bacterial communities at these hydrothermal vent systems. The overarching goal of this study is to evaluate and compare taxonomic and metabolic diversity among different communities of microbial mats. We compared communities collected on a fine scale to analyze the bacterial community based on gross mat morphology, geography, and nearby vent effluent chemistry. Taxa richness and evenness are compared with rarefaction curves to visualize diversity. As well as providing a survey of diversity this study also presents a juxtaposition of three methods in which ribosomal small subunit diversity is compared with T-RFLP, next generation amplicon sequencing, and metagenomic shotgun sequencing.

  10. Two possible hydrothermal vents in the northern Oki-nawa Trough

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    As the Okinawa Trough is a back-arc basin in early spreading, modern submarine hydrothermal activity and minerallization have many characteristics which have aroused wide attention. Up to now, three well-known hy-drothermal venting areas are all located in the middle part of the trough. During two cruise investigations to map and sample the seafloor, numbers of Calyptogena sp. shells were dredged at two sites in the northern trough with compara-tively thicker crust and numerous submarine volcanoes. Based on the fact that Calyptogena sp. is only observed around the hydrothermal vents and lives on hydrothermal activities, it is predicted that there is the possibility of mod-ern hydrothermal activities in the northern part of the trough. In this note, the shell is carefully characterized and the sample locations with possible hydrothermal activity are given. It is pointed out that the research of biogenic fossils to trace hydrothermal activity changes in venting time, strength fluctuations, evolution in chemical compositions and so on should be stressed in the future in addition to the study of the ecological characteristics of hydrothermal organisms.

  11. The potential for photosynthesis in hydrothermal vents: a new avenue for life in the Universe?

    CERN Document Server

    Perez, Noel; Martin, Osmel; Leiva-Mora, Michel

    2013-01-01

    We perform a quantitative assessment for the potential for photosynthesis in hydrothermal vents in the deep ocean. The photosynthetically active radiation in this case is from geothermal origin: the infrared thermal radiation emitted by hot water, at temperatures ranging from 473 up to 673 K. We find that at these temperatures the photosynthetic potential is rather low in these ecosystems for most known species. However, species which a very high efficiency in the use of light and which could use infrared photons till 1300nm, could achieve good rates of photosynthesis in hydrothermal vents. These organisms might also thrive in deep hydrothermal vents in other planetary bodies, such as one of the more astrobiologically promising Jupiter satellites: Europa.

  12. Novel Forms of Structural Integration between Microbes and a Hydrothermal Vent Gastropod from the Indian Ocean

    OpenAIRE

    Goffredi, Shana K.; Warén, Anders; Orphan, Victoria J; Van Dover, Cindy L.; Vrijenhoek, Robert C

    2004-01-01

    Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of {gamma}-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting differs from that of all other vent mollusks, which harbor sulfur-oxidizing endosymbionts in their gills. The significantly reduced digestive tract, the isotopic signatures of the ...

  13. Temporal and spatial variation in temperature experienced by macrofauna at main endeavour Hydrothermal vent field

    OpenAIRE

    Lee, Raymond W.; Robert, Katleen; Matabos, Marjolaine; Bates, Amanda E.; Juniper, S. Kim

    2015-01-01

    A significant focus of hydrothermal vent ecological studies has been to understand how species cope with various stressors through physiological tolerance and biochemical resistance. Yet, the environmental conditions experienced by vent species have not been well characterized. This objective requires continuous observations over time intervals that can capture environmental variability at scales that are relevant to animals. We used autonomous temperature logger arrays (four roughly parallel...

  14. Hydrothermal Vents at 5000m on the Mid-Cayman Rise: The Deepest and Hottest Hydrothermal Systems Yet Discovered!

    Science.gov (United States)

    Murton, B. J.; Connelly, D. P.; Copley, J. T.; Stansfield, K. L.; Tyler, P. A.; Cruise Jc044 Sceintific Party

    2010-12-01

    This contribution describes the geological setting of hydrothermal activity within the Mid- Cayman Rise (MCR) using data acquired during cruise JC044 (MAR-APR 2010) from the deep-towed sidescan sonar TOBI, AUV Autosub6000 and the ROTV HyBIS. The 110 km-long Mid- Cayman Rise (MCR), located within Caribbean Sea, is the deepest spreading centre known, reaching over 6000m. Hence it poses an end-member of extreme depth for hydrothermal circulation. Accretion of new volcanic crust is focused within two ridge segments, to the north and south of a centrally located massif of peridotite and gabbro. Following earlier indications of hydrothermal plumes (German et al., in 2009), we discovered two high-temperature hydrothermal system: one at a depth of 5000m in the neovolcanic zone of the northern segment, and another at 2300m on the flanks of the MCR. These sites show contrasting styles of fluid venting, mineralisation, geological setting and host rock interaction. At 5000m-depth, the ultra-deep vent site forms the deepest hydrothermal system known. Venting is focused at the western side of a 100m diameter, 30m high mound, while inactive sulphides extend eastwards for at least 800m. Fluids discharge from clusters of chimneys whose location is related to basement faults. Changes in salinity in the venting fluids indicate discharge of a low salinity phase and a brine phase. At 500bar, this is definitive evidence for supercritical fluid emission. We also found the sulphide mineralization to be copper-rich, giving a characteristic green hue to many of the deposits, probably a result of the super-critical state of the vent fluids. A prominent axial volcanic ridge nearby indicates a robust magma supply to the northern MCR segment. Thus it is likely the ultra-deep vent site derives its thermal energy from magmatic sources, similar to those thought to underlie other slow-spreading ridge volcanic-hosted vent sites (e.g. Broken Spur: MAR). The shallower (2300m) MCR hydrothermal vent

  15. Genomic Reconstruction of an Uncultured Hydrothermal Vent Gammaproteobacterial Methanotroph (Family Methylothermaceae) Indicates Multiple Adaptations to Oxygen Limitation

    OpenAIRE

    Skennerton, Connor T.; Ward, Lewis M.; Michel, Alice; Metcalfe, Kyle; Valiente, Chanel; Mullin, Sean; Chan, Ken Y.; Gradinaru, Viviana; Orphan, Victoria J

    2015-01-01

    Hydrothermal vents are an important contributor to marine biogeochemistry, producing large volumes of reduced fluids, gasses, and metals and housing unique, productive microbial and animal communities fueled by chemosynthesis. Methane is a common constituent of hydrothermal vent fluid and is frequently consumed at vent sites by methanotrophic bacteria that serve to control escape of this greenhouse gas into the atmosphere. Despite their ecological and geochemical importance, little is known a...

  16. Differences in recovery between deep-sea hydrothermal vent and vent-proximate communities after a volcanic eruption

    Science.gov (United States)

    Gollner, Sabine; Govenar, Breea; Arbizu, Pedro Martinez; Mills, Susan; Le Bris, Nadine; Weinbauer, Markus; Shank, Timothy M.; Bright, Monika

    2015-12-01

    Deep-sea hydrothermal vents and the surrounding basalt seafloor are subject to major natural disturbance events such as volcanic eruptions. In the near future, anthropogenic disturbance in the form of deep-sea mining could also significantly affect the faunal communities of hydrothermal vents. In this study, we monitor and compare the recovery of insular, highly productive vent communities and vent-proximate basalt communities following a volcanic eruption that destroyed almost all existing communities at the East Pacific Rise, 9°50‧N in 2006. To study the recovery patterns of the benthic communities, we placed settlement substrates at vent sites and their proximate basalt areas and measured the prokaryotic abundance and compared the meio- and macrofaunal species richness and composition at one, two and four years after the eruption. In addition, we collected samples from the overlying water column with a pelagic pump, at one and two years after the volcanic eruption, to determine the abundance of potential meiofauna colonisers. One year after eruption, mean meio- and macrofaunal abundances were not significantly different from pre-eruption values in vent habitats (meio: 8-1838 ind. 64 cm-2 in 2006; 3-6246 ind. 64 cm-2 in 2001/02; macro: 95-1600 ind. 64 cm-2 in 2006; 205-4577 ind. 64 cm-2 in 2001/02) and on non-vent basalt habitats (meio: 10-1922 ind. 64 cm-2 in 2006; 8-328 ind. 64 cm-2 in 2003/04; macro: 14-3351 ind. 64 cm-2 in 2006; 2-63 ind. 64 cm-2 in 2003/04), but species recovery patterns differed between the two habitat types. In the vent habitat, the initial community recovery was relatively quick but incomplete four years after eruption, which may be due to the good dispersal capabilities of vent endemic macrofauna and vent endemic dirivultid copepods. At vents, 42% of the pre-eruption meio- and 39% of macrofaunal species had returned. In addition, some new species not evident prior to the eruption were found. At the tubeworm site Tica, a total of 26

  17. Microbial geochemistry in rising plumes of two hydrothermal vents at the Mid-Cayman Rise

    Science.gov (United States)

    Dick, G.; Breier, J. A.; Toner, B. M.; Sheik, C.; Cron, B. R.; Li, M.; Reed, D. C.; Anantharaman, K.; Baker, B. J.; Jain, S.; Klausmeier, C. A.; Jiang, H.; German, C. R.; Seewald, J.; Sylva, S. P.; McDermott, J. M.; Bennett, S. A.

    2012-12-01

    Slow and ultraslow spreading ridges comprise ~50% of the global ridge-axis length and are thus relevant to the impact of hydrothermal activity on global ocean biogeochemistry. These ridges host ultramafic vent systems with reducing chemical environments that yield hydrothermal fluids rich in methane, hydrogen, and organic carbon, thus providing energy sources to biological communities relevant to the origin and early evolution of life on Earth and the potential for life on other planets. Microbial-geochemical interactions are also important for understanding how deep-sea hydrothermal vents impact ocean biogeochemistry, especially in hydrothermal plumes, where vent fluids stimulate chemosynthetic microbial communities and microbes influence the oceanic fate of hydrothermally-sourced elements. Many critical processes occur in the rising portion of hydrothermal plumes, which are dynamic and challenging to sample. To address these questions and challenges, we developed methods for the in situ collection and preservation of paired microbiology and geochemical samples from rising hydrothermal plumes. Samples were collected with ROV Jason from two hydrothermal vent systems, Von Damm and Beebe, which are in close proximity to each other on the Mid Cayman Rise yet are quite distinct in terms of chemistry, temperature, and depth. Bulk geochemistry, spatially-resolved spectroscopy, and molecular microbiological approaches were applied to yield some of the first views into the dynamic biotic and abiotic processes operative in rising hydrothermal plumes from an ultra-slow spreading system. Results indicate that the Cayman plumes are enriched in hydrogen, sulfur, and methane-utilizing microorganisms relative to background deep Caribbean seawater. Clear differences were observed between near-vent samples, which were dominated by seafloor-derived organisms, and samples from the upper buoyant or non-buoyant plume. These Cayman plume microbes are distinct from those observed in

  18. Carbon fluxes from hydrothermal vents off Milos, Aegean Volcanic Arc, and the influence of venting on the surrounding ecosystem.

    Science.gov (United States)

    Dando, Paul; Aliani, Stefano; Bianchi, Nike; Kennedy, Hilary; Linke, Peter; Morri, Carla

    2014-05-01

    The island of Milos, in the Aegean Sea, has extensive hydrothermal fields to the east and southeast of the island with additional venting areas near the entrance to and within the central caldera. A calculation of the total area of the vent fields, based on ship and aerial surveys, suggested that the hydrothermal fields occupy 70 km2, twice the area previously estimated. The vents ranged in water depth from the intertidal to 300 m. As a result of the low depths there was abundant free gas release: in places water boiled on the seabed. The stream of gas bubbles rising through the sandy seabed drove a shallow re-circulation of bottom seawater. The majority of the water released with the gas, with a mean pH of 5.5, was re-circulated bottom water that had become acidified in contact with CO2 gas and was often diluted by admixture with the vapour phase from the deeper fluids. The major component of the free gas, 80%, was CO2, with an estimated total flux of 1.5-7.5 x 1012 g a-1. The methane flux, by comparison, was of the order of 1010 g a.-1 Using methane as a tracer it was shown that the major gas export from the vents was below the thermocline towards the southwest, in agreement with the prevailing currents. Areas of hydrothermal brine seepage occurred between the gas vents and occasional brine pools were observed in seabed depressions. Under relatively calm conditions, many of the brine seeps were covered by thick minero-bacterial mats consisting of silica and sulphur and surrounded by mats of diatoms and cyanobacteria. The minerals were not deposited in the absence of bacteria. Storms disrupted the mats, leading to an export of material to the surrounding area. Stable isotope data from sediments and sediment trap material suggested that exported POM was processed by zooplankton. The combined effects of the geothermal heating of the seabed, the large gas flux, variation in the venting and the effect of the brine seeps had a dramatic effect on the surrounding

  19. How many vent fields? New estimates of vent field populations on ocean ridges from precise mapping of hydrothermal discharge locations

    Science.gov (United States)

    Baker, Edward T.; Resing, Joseph A.; Haymon, Rachel M.; Tunnicliffe, Verena; Lavelle, J. William; Martinez, Fernando; Ferrini, Vicki; Walker, Sharon L.; Nakamura, Koichi

    2016-09-01

    Decades of exploration for venting sites along spreading ridge crests have produced global datasets that yield estimated mean site spacings of ∼ 12- 220 km. This conclusion demands that sites where hydrothermal fluid leaks from the seafloor are improbably rare along the 66 000 km global ridge system, despite the high bulk permeability of ridge crest axes. However, to date, exploration methods have neither reliably detected plumes from isolated low-temperature, particle-poor, diffuse sources, nor differentiated individual, closely spaced (clustered within a few kilometers) sites of any kind. Here we describe a much lower mean discharge spacing of 3-20 km, revealed by towing real-time oxidation-reduction-potential and optical sensors continuously along four fast- and intermediate-rate (>55 mm/yr) spreading ridge sections totaling 1470 km length. This closer spacing reflects both discovery of isolated sites discharging particle-poor plumes (25% of all sites) and improved discrimination (at a spatial resolution of ∼1 km) among clustered discrete and diffuse sources. Consequently, the number of active vent sites on fast- and intermediate-rate spreading ridges may be at least a factor of 3-6 higher than now presumed. This increase provides new quantitative constraints for models of seafloor processes such as dispersal of fauna among seafloor and crustal chemosynthetic habitats, biogeochemical impacts of diffuse venting, and spatial patterns of hydrothermal discharge.

  20. How many vent fields? New estimates of vent field populations on ocean ridges from precise mapping of hydrothermal discharge locations

    Science.gov (United States)

    Baker, Edward T.; Resing, Joseph A.; Haymon, Rachel M.; Tunnicliffe, Verena; Lavelle, J. William; Martinez, Fernando; Ferrini, Vicki; Walker, Sharon L.; Nakamura, Koichi

    2016-09-01

    Decades of exploration for venting sites along spreading ridge crests have produced global datasets that yield estimated mean site spacings of ∼ 12- 220 km. This conclusion demands that sites where hydrothermal fluid leaks from the seafloor are improbably rare along the 66 000 km global ridge system, despite the high bulk permeability of ridge crest axes. However, to date, exploration methods have neither reliably detected plumes from isolated low-temperature, particle-poor, diffuse sources, nor differentiated individual, closely spaced (clustered within a few kilometers) sites of any kind. Here we describe a much lower mean discharge spacing of 3-20 km, revealed by towing real-time oxidation-reduction-potential and optical sensors continuously along four fast- and intermediate-rate (>55 mm/yr) spreading ridge sections totaling 1470 km length. This closer spacing reflects both discovery of isolated sites discharging particle-poor plumes (25% of all sites) and improved discrimination (at a spatial resolution of ∼1 km) among clustered discrete and diffuse sources. Consequently, the number of active vent sites on fast- and intermediate-rate spreading ridges may be at least a factor of 3-6 higher than now presumed. This increase provides new quantitative constraints for models of seafloor processes such as dispersal of fauna among seafloor and crustal chemosynthetic habitats, biogeochemical impacts of diffuse venting, and spatial patterns of hydrothermal discharge.

  1. COVIS Detects Interconnections Between Atmospheric, Oceanic and Geologic systems at a Deep Sea Hydrothermal Vent

    Science.gov (United States)

    Bemis, K. G.; Xu, G.; Lee, R.

    2015-12-01

    COVIS (Cabled Observatory Vent Imaging Sonar) is an innovative sonar system designed to quantitatively monitor focused and diffuse flows from deep-sea hydrothermal vent clusters. From 9/2010 to 9/2015, COVIS was connected to the NEPTUNE observatory at Grotto vent in the Main Endeavour Field, JdFR. COVIS monitored plumes and diffuse discharge by transmitting high-frequency (200-400 kHz), pulsed acoustic waves and recording the backscattered signals to yield time series of plume heat and volume transports, plume bending, and diffuse flow area. Temporal variations indicate the rate of hydrothermal plume mixing with the ambient seawater increases with the magnitude of ocean currents. Such current-driven entrainment links the dynamics of a deep-sea hydrothermal plume with oceanic and atmospheric processes. We estimate the direction and relative amplitude of the local bottom currents from the bending angles of the plumes. A comparison with currents from an ADCP (~80 m south of Grotto) reveals significant complexity in the mean bottom flow structure within a hydrothermal vent field. Diffuse flow area, temperature, and faunal densities vary periodically reflecting some combination of tidal pressure and current interactions. The heat transport time series suggests the heat source driving the plume remained relatively steady for 41 months. Local seismic data reveals that increased heat transport in 2000 followed seismic events in 1999 and 2000 and the steady heat flux from 10/2011 to 2/2015 coincided with quiescent seismicity. Such a correlation points to the close linkage of a seafloor hydrothermal system with geological processes. These findings demonstrate the intimate interconnections of seafloor hydrothermal systems with processes spanning the Earth's interior to the sea surface. Further, they (and the time-series acquired by COVIS) testify to the effectiveness and robustness of employing an acoustic-imaging sonar for long-term monitoring of a seafloor hydrothermal

  2. Processes and interactions in macrofaunal assemblages at hydrothermal vents: A modeling perspective

    Science.gov (United States)

    Shea, Katriona; Metaxas, Anna; Young, Curtis R.; Fisher, Charles R.

    Our understanding of the biological assemblages at hydrothermal vents is growing rapidly, in part facilitated by the coupling of experimental and observational methods with theoretical modeling efforts. We review theoretical approaches in four main areas and link them to empirical studies of the biology of hydrothermal vent systems. First, we describe models of dispersal of vent organisms within and among vent sites. These models fall broadly into two categories: those based on larval biology and currents that predict dispersal capabilities from a source, and those using genetic data to address historical genetic links between populations. Then we discuss models for individual and population growth at a location and give examples from work in a biologically similar deep-sea environment: cold seeps. Third, we address how these two aspects can be integrated using models that couple dispersal and demography. Last, we summarize existing modeling approaches for community succession in other types of habitats, and outline the conceptual models and the sorts of data that currently exist for hydrothermal vent communities. Throughout, we also examine the use of models in improving experimental design.

  3. Bacterial sulfur cycle shapes microbial communities in surface sediments of an ultramafic hydrothermal vent field

    DEFF Research Database (Denmark)

    Schauer, Regina; Røy, Hans; Augustin, Nico;

    2011-01-01

    The ultramafic-hosted Logatchev hydrothermal field (LHF) is characterized by vent fluids, which are enriched in dissolved hydrogen and methane compared with fluids from basalt-hosted systems. Thick sediment layers in LHF are partly covered by characteristic white mats. In this study, these...

  4. Bacterial Sulfate Reduction Above 100-Degrees-C in Deep-Sea Hydrothermal Vent Sediments

    DEFF Research Database (Denmark)

    JØRGENSEN, BB; ISAKSEN, MF; JANNASCH, HW

    1992-01-01

    -reducing bacteria was done in hot deep-sea sediments at the hydrothermal vents of the Guaymas Basin tectonic spreading center in the Gulf of California. Radiotracer studies revealed that sulfate reduction can occur at temperatures up to 110-degrees-C, with an optimum rate at 103-degrees to 106-degrees-C. This...

  5. Phylogenetic characterization of episymbiotic bacteria hosted by a hydrothermal vent limpet (Lepetodrilidae, Vetigastropoda)

    NARCIS (Netherlands)

    Bates, A.E.; Harme, T.L.; Roeselers, G.; Cavanaugh, C.M.

    2011-01-01

    Marine invertebrates hosting chemosynthetic bacterial symbionts are known from multiple phyla and represent remarkable diversity in form and function. The deep-sea hydrothermal vent limpet Lepetodrilus fucensis from the Juan de Fuca Ridge complex hosts a gill symbiosis of particular interest because

  6. Bottom sediments and pore waters near a hydrothermal vent in Lake Baikal (Frolikha Bay)

    Science.gov (United States)

    Granina, L.Z.; Klerkx, J.; Callender, E.; Leermakers, M.; Golobokova, L.P.

    2007-01-01

    We discuss the redox environments and the compositions of bottom sediments and sedimentary pore waters in the region of a hydrothermal vent in Frolikha Bay, Lake Baikal. According to our results, the submarine vent and its companion nearby spring on land originate from a common source. The most convincing evidence for their relation comes from the proximity of stable oxygen and hydrogen isotope compositions in pore waters and in the spring water. The isotope composition indicates a meteoric origin of pore waters, but their major- and minor-element chemistry bears imprint of deep water which may seep through permeable faulted crust. Although pore waters near the submarine vent have a specific enrichment in major and minor constituents, hydrothermal discharge at the Baikal bottom causes a minor impact on the lake water chemistry, unlike the case of freshwater geothermal lakes in the East-African Rift and North America. ?? 2007.

  7. The importance of hydrothermal venting to water-column secondary production in the northeast Pacific

    Science.gov (United States)

    Burd, Brenda J.; Thomson, Richard E.

    2015-11-01

    The purpose of this study is to show that seafloor hydrothermal venting in the open northeast Pacific Ocean has a marked impact on secondary biomass and production within the overlying water column. Specifically, we use net tows and concurrently measured acoustic backscatter data collected over six summers to examine the effects of hydrothermal venting from the Endeavour Segment of Juan de Fuca Ridge on macro-zooplankton biomass and production throughout the entire 2000 m depth range. Previous research shows that ontogenetic diapausing migrators and their predators from the upper ocean aggregate above the neutrally buoyant plumes in summer and resume feeding on plume and bottom upwelled particles, resulting in increased zooplankton reproductive output to the upper ocean. Within the limitations of our sampling methodology, net tows reveal a statistically significant exponential decline in total water-column biomass with increasing lateral distance from the vent fields. The acoustic backscatter data show a similar decline, but only below 800 m depth. Near-surface biomass was highly variable throughout the region, but values near vents consistently ranged higher than summer values found elsewhere in the offshore northeast Pacific. Water-column biomass was similar in magnitude above and below 800 m depth throughout the region. Because epiplume biomass can be advected a considerable distance from vent fields, biomass enhancement of the water column from hydrothermal venting may extend considerable distances to the west and northwest of the vent sites, in the prevailing directions of the subsurface flow. Based on the extensive acoustic Doppler current profiler (ADCP) data collected, and the strong correlation between zooplankton production derived from net sample biomass and acoustic backscatter intensity, we estimate that daily macro-zooplankton production in the upper 400 m of the water column within 10 km of the vent fields averages approximately 16% of photosynthetic

  8. Quantifying dispersal from hydrothermal vent fields in the western Pacific Ocean.

    Science.gov (United States)

    Mitarai, Satoshi; Watanabe, Hiromi; Nakajima, Yuichi; Shchepetkin, Alexander F; McWilliams, James C

    2016-03-15

    Hydrothermal vent fields in the western Pacific Ocean are mostly distributed along spreading centers in submarine basins behind convergent plate boundaries. Larval dispersal resulting from deep-ocean circulations is one of the major factors influencing gene flow, diversity, and distributions of vent animals. By combining a biophysical model and deep-profiling float experiments, we quantify potential larval dispersal of vent species via ocean circulation in the western Pacific Ocean. We demonstrate that vent fields within back-arc basins could be well connected without particular directionality, whereas basin-to-basin dispersal is expected to occur infrequently, once in tens to hundreds of thousands of years, with clear dispersal barriers and directionality associated with ocean currents. The southwest Pacific vent complex, spanning more than 4,000 km, may be connected by the South Equatorial Current for species with a longer-than-average larval development time. Depending on larval dispersal depth, a strong western boundary current, the Kuroshio Current, could bridge vent fields from the Okinawa Trough to the Izu-Bonin Arc, which are 1,200 km apart. Outcomes of this study should help marine ecologists estimate gene flow among vent populations and design optimal marine conservation plans to protect one of the most unusual ecosystems on Earth. PMID:26929376

  9. High levels of natural radioactivity in biota from deep-sea hydrothermal vents: a preliminary communication

    International Nuclear Information System (INIS)

    Hydrothermal deep-sea vent fauna is naturally exposed to a peculiar environment enriched in potentially toxic species such as sulphides, heavy metals and natural radionuclides. It is now well established that some of the organisms present in such an environment accumulate metals during their lifespan. Though only few radionuclide measurements are available, it seems likely that hydrothermal vent communities are exposed to high natural radiation doses. Various archived biological samples collected on the East Pacific Rise and the Mid-Atlantic Ridge in 1996, 2001 and 2002 were analysed by ICP-MS in order to determine their uranium contents (238U, 235U and 234U). In addition 210Po-Pb were determined in 2 samples collected in 2002. Vent organisms are characterized by high U, and Po-Pb levels compared to what is generally encountered in organisms from outside hydrothermal vent ecosystems. Though the number of data is low, the results reveal various trends in relation to the site, the location within the mixing zone and/or the organisms' trophic regime.

  10. High levels of natural radioactivity in biota from deep-sea hydrothermal vents: a preliminary communication.

    Science.gov (United States)

    Charmasson, Sabine; Sarradin, Pierre-Marie; Le Faouder, Antoine; Agarande, Michèle; Loyen, Jeanne; Desbruyères, Daniel

    2009-06-01

    Hydrothermal deep-sea vent fauna is naturally exposed to a peculiar environment enriched in potentially toxic species such as sulphides, heavy metals and natural radionuclides. It is now well established that some of the organisms present in such an environment accumulate metals during their lifespan. Though only few radionuclide measurements are available, it seems likely that hydrothermal vent communities are exposed to high natural radiation doses. Various archived biological samples collected on the East Pacific Rise and the Mid-Atlantic Ridge in 1996, 2001 and 2002 were analysed by ICP-MS in order to determine their uranium contents ((238)U, (235)U and (234)U). In addition (210)Po-Pb were determined in 2 samples collected in 2002. Vent organisms are characterized by high U, and Po-Pb levels compared to what is generally encountered in organisms from outside hydrothermal vent ecosystems. Though the number of data is low, the results reveal various trends in relation to the site, the location within the mixing zone and/or the organisms' trophic regime. PMID:19362761

  11. Evolutionary strategies of viruses, bacteria and archaea in hydrothermal vent ecosystems revealed through metagenomics.

    Directory of Open Access Journals (Sweden)

    Rika E Anderson

    Full Text Available The deep-sea hydrothermal vent habitat hosts a diverse community of archaea and bacteria that withstand extreme fluctuations in environmental conditions. Abundant viruses in these systems, a high proportion of which are lysogenic, must also withstand these environmental extremes. Here, we explore the evolutionary strategies of both microorganisms and viruses in hydrothermal systems through comparative analysis of a cellular and viral metagenome, collected by size fractionation of high temperature fluids from a diffuse flow hydrothermal vent. We detected a high enrichment of mobile elements and proviruses in the cellular fraction relative to microorganisms in other environments. We observed a relatively high abundance of genes related to energy metabolism as well as cofactors and vitamins in the viral fraction compared to the cellular fraction, which suggest encoding of auxiliary metabolic genes on viral genomes. Moreover, the observation of stronger purifying selection in the viral versus cellular gene pool suggests viral strategies that promote prolonged host integration. Our results demonstrate that there is great potential for hydrothermal vent viruses to integrate into hosts, facilitate horizontal gene transfer, and express or transfer genes that manipulate the hosts' functional capabilities.

  12. Evolutionary strategies of viruses, bacteria and archaea in hydrothermal vent ecosystems revealed through metagenomics.

    Science.gov (United States)

    Anderson, Rika E; Sogin, Mitchell L; Baross, John A

    2014-01-01

    The deep-sea hydrothermal vent habitat hosts a diverse community of archaea and bacteria that withstand extreme fluctuations in environmental conditions. Abundant viruses in these systems, a high proportion of which are lysogenic, must also withstand these environmental extremes. Here, we explore the evolutionary strategies of both microorganisms and viruses in hydrothermal systems through comparative analysis of a cellular and viral metagenome, collected by size fractionation of high temperature fluids from a diffuse flow hydrothermal vent. We detected a high enrichment of mobile elements and proviruses in the cellular fraction relative to microorganisms in other environments. We observed a relatively high abundance of genes related to energy metabolism as well as cofactors and vitamins in the viral fraction compared to the cellular fraction, which suggest encoding of auxiliary metabolic genes on viral genomes. Moreover, the observation of stronger purifying selection in the viral versus cellular gene pool suggests viral strategies that promote prolonged host integration. Our results demonstrate that there is great potential for hydrothermal vent viruses to integrate into hosts, facilitate horizontal gene transfer, and express or transfer genes that manipulate the hosts' functional capabilities. PMID:25279954

  13. Cameras on the NEPTUNE Canada seafloor observatory: Towards monitoring hydrothermal vent ecosystem dynamics

    Science.gov (United States)

    Robert, K.; Matabos, M.; Sarrazin, J.; Sarradin, P.; Lee, R. W.; Juniper, K.

    2010-12-01

    Hydrothermal vent environments are among the most dynamic benthic habitats in the ocean. The relative roles of physical and biological factors in shaping vent community structure remain unclear. Undersea cabled observatories offer the power and bandwidth required for high-resolution, time-series study of the dynamics of vent communities and the physico-chemical forces that influence them. The NEPTUNE Canada cabled instrument array at the Endeavour hydrothermal vents provides a unique laboratory for researchers to conduct long-term, integrated studies of hydrothermal vent ecosystem dynamics in relation to environmental variability. Beginning in September-October 2010, NEPTUNE Canada (NC) will be deploying a multi-disciplinary suite of instruments on the Endeavour Segment of the Juan de Fuca Ridge. Two camera and sensor systems will be used to study ecosystem dynamics in relation to hydrothermal discharge. These studies will make use of new experimental protocols for time-series observations that we have been developing since 2008 at other observatory sites connected to the VENUS and NC networks. These protocols include sampling design, camera calibration (i.e. structure, position, light, settings) and image analysis methodologies (see communication by Aron et al.). The camera systems to be deployed in the Main Endeavour vent field include a Sidus high definition video camera (2010) and the TEMPO-mini system (2011), designed by IFREMER (France). Real-time data from three sensors (O2, dissolved Fe, temperature) integrated with the TEMPO-mini system will enhance interpretation of imagery. For the first year of observations, a suite of internally recording temperature probes will be strategically placed in the field of view of the Sidus camera. These installations aim at monitoring variations in vent community structure and dynamics (species composition and abundances, interactions within and among species) in response to changes in environmental conditions at different

  14. Estimation of heat and chemical fluxes from a seafloor hydrothermal vent field using radon measurements

    Energy Technology Data Exchange (ETDEWEB)

    Rosenberg, N.D.; Lupton, J.E.; Kadko, D.; Collier, R.; Lilley, M.D.; Pak, H.

    1988-08-18

    The circulation of seawater through newly formed ocean crust at mid-ocean ridge spreading centres is important in the oceanic heat and chemical budgets. Here we introduce a geochemical approach to estimating the flux from a hydrothermal vent field based on radon (/sup 222/Rn) measurements in the overlying effluent plume. This method was applied successfully in 1986 during a 23-day expedition to an active vent field on the 170-km Endeavour segment of the Juan de Fuca Ridge. We estimate the heat flux from this site to be 1-5 x 10/sup 9/ W.

  15. Dynamic drivers of a shallow-water hydrothermal vent ecogeochemical system (Milos, Eastern Mediterranean)

    Science.gov (United States)

    Yücel, Mustafa; Sievert, Stefan; Giovanelli, Donato; Foustoukos, Dionysis; DeForce, Emelia; Thomas, François; Vetriani, Constantino; Le Bris, Nadine

    2014-05-01

    Shallow-water hydrothermal vents share many characteristics with their deep-sea analogs. However, despite ease of access, much less is known about the dynamics of these systems. Here, we report on the spatial and temporal chemical variability of a shallow-water vent system at Paleochori Bay, Milos Island, Greece, and on the bacterial and archaeal diversity of associated sandy sediments. Our multi-analyte voltammetric profiles of dissolved O2 and hydrothermal tracers (e.g. Fe2+, FeSaq, Mn2+) on sediment cores taken along a transect in hydrothermally affected sediments indicate three different areas: the central vent area (highest temperature) with a deeper penetration of oxygen into the sediment, and a lack of dissolved Fe2+ and Mn2+; a middle area (0.5 m away) rich in dissolved Fe2+ and Mn2+ (exceeding 2 mM) and high free sulfide with potential for microbial sulfide oxidation as suggested by the presence of white mats at the sediment surface; and, finally, an outer rim area (1-1.5 m away) with lower concentrations of Fe2+ and Mn2+ and higher signals of FeSaq, indicating an aged hydrothermal fluid contribution. In addition, high-frequency temperature series and continuous in situ H2S measurements with voltammetric sensors over a 6-day time period at a distance 0.5 m away from the vent center showed substantial temporal variability in temperature (32 to 46 ºC ) and total sulfide (488 to 1329 µM) in the upper sediment layer. Analysis of these data suggests that tides, winds, and abrupt geodynamic events generate intermittent mixing conditions lasting for several hours to days. Despite substantial variability, the concentration of sulfide available for chemoautotrophic microbes remained high. These findings are consistent with the predominance of Epsilonproteobacteria in the hydrothermally influenced sediments Diversity and metagenomic analyses on sediments and biofilm collected along a transect from the center to the outer rim of the vent provide further insights on

  16. The discovery of new deep-sea hydrothermal vent communities in the southern ocean and implications for biogeography.

    Directory of Open Access Journals (Sweden)

    Alex D Rogers

    2012-01-01

    Full Text Available Since the first discovery of deep-sea hydrothermal vents along the Galápagos Rift in 1977, numerous vent sites and endemic faunal assemblages have been found along mid-ocean ridges and back-arc basins at low to mid latitudes. These discoveries have suggested the existence of separate biogeographic provinces in the Atlantic and the North West Pacific, the existence of a province including the South West Pacific and Indian Ocean, and a separation of the North East Pacific, North East Pacific Rise, and South East Pacific Rise. The Southern Ocean is known to be a region of high deep-sea species diversity and centre of origin for the global deep-sea fauna. It has also been proposed as a gateway connecting hydrothermal vents in different oceans but is little explored because of extreme conditions. Since 2009 we have explored two segments of the East Scotia Ridge (ESR in the Southern Ocean using a remotely operated vehicle. In each segment we located deep-sea hydrothermal vents hosting high-temperature black smokers up to 382.8°C and diffuse venting. The chemosynthetic ecosystems hosted by these vents are dominated by a new yeti crab (Kiwa n. sp., stalked barnacles, limpets, peltospiroid gastropods, anemones, and a predatory sea star. Taxa abundant in vent ecosystems in other oceans, including polychaete worms (Siboglinidae, bathymodiolid mussels, and alvinocaridid shrimps, are absent from the ESR vents. These groups, except the Siboglinidae, possess planktotrophic larvae, rare in Antarctic marine invertebrates, suggesting that the environmental conditions of the Southern Ocean may act as a dispersal filter for vent taxa. Evidence from the distinctive fauna, the unique community structure, and multivariate analyses suggest that the Antarctic vent ecosystems represent a new vent biogeographic province. However, multivariate analyses of species present at the ESR and at other deep-sea hydrothermal vents globally indicate that vent biogeography is more

  17. Evolution of respiratory adaptations in hydrothermal vent scale-worms (Polynoidae)

    OpenAIRE

    Projecto‑Garcia, Joana Cristina

    2009-01-01

    Hydrothermal vents are deep-sea ecosystems that are characterized by widely changing chemical and physical characteristics that are the result of the chaotic mixing of the hydrothermal fluid, rich in toxic compounds, such as sulfide and heavy metals, with low pH, high temperature and no oxygen; with the deep-sea water that contains no heavy metals, has a pH of ~7.8, low temperature and normal oxygen concentrations. Among others constraints, hypoxia and anoxia pose a serious problem for the fa...

  18. [Comment on “Submarine hot springs: Origin of life?”] Hydrothermal vents revisited

    Science.gov (United States)

    Hoffman, Sarah

    It was gratifying to read Peter Bell's synopsis of our paper [Corliss et al., 1981] in the March 23 issue of Eos (Submarine hot springs: Origin of life?) however, in the last sentence, he wrote, ‘They note that microorganisms found in recent expeditions to the submarine hot springs of the East Pacific Rise would be evidence that the processes are still occurring.’In our final paragraph we actually said that while “events leading to the formation of complex organic compounds and “protocell” structures may still be occurring in present-day oceanic hydrothermal systems … the complex communities of bacteria in modern oceanic environments would outcompete and consume abiotically synthesized protocells…” Modern-day vent microbiota will probably mask or destroy any evidence for abiotic synthesis in the hydrothermal vents.

  19. Study on the High Precision Acoustic Measurement Techniques for Determining Temperature Field Around Seafloor Hydrothermal Vent

    Institute of Scientific and Technical Information of China (English)

    CAI Yong; FAN Wei; ZHOU Yan; FU Xian-qiao; FANG Hui; JIN Tao

    2012-01-01

    This paper presents the basis of acoustic method used for temperature field measurement of seafloor hydrothermal vent and two techniques of the parabolic interpolation and the bending compensation of propagation paths of acoustic signal are introduced.Experimental research is performed to exactly rebuild the temperature field around hot springs on the floor of Qiezishan Lake,Yunnan,China.The accuracy of the travel time estimation has been improved based on the aforementioned technique and method.At the same time,by comparison of the results of temperature field with different means,the max absolute error,the maximum relative error and the root mean square error are given.It shows that the technique and the method presented in the paper can be applied to the temperature field measurement detector around the seafloor hydrothermal vent.It also has a good accuracy.

  20. Bioavailable metals and cellular effects in the digestive gland of marine limpets living close to shallow water hydrothermal vents.

    OpenAIRE

    Cunha, Luís; Amaral, André; Medeiros, Vera; Martins, Gustavo M.; Wallenstein, Francisco; Couto, Ruben P.; Neto, Ana I.; Rodrigues, Armindo

    2008-01-01

    Copyright © 2007 Elsevier Ltd All rights reserved. The pressure exerted by shallow water hydrothermal vents on edible gastropods and their cellular responses triggered by these stresses are almost unknown. The aims of this study were to evaluate the bioavailability of metals in the Macaronesian endemic limpet Patella candei gomesii living close to shallow water hydrothermal vents, and the structural differences in their digestive gland as well as the levels of apoptosis in that organ. L...

  1. Expression patterns of mRNAs for methanotrophy and thiotrophy in symbionts of the hydrothermal vent mussel Bathymodiolus puteoserpentis

    OpenAIRE

    Wendeberg, Annelie; Zielinski, Frank U.; Borowski, Christian; Dubilier, Nicole

    2011-01-01

    The hydrothermal vent mussel Bathymodiolus puteoserpentis (Mytilidae) from the Mid-Atlantic Ridge hosts symbiotic sulfur- and methane-oxidizing bacteria in its gills. In this study, we investigated the activity and distribution of these two symbionts in juvenile mussels from the Logatchev hydrothermal vent field (14°45′N Mid-Atlantic Ridge). Expression patterns of two key genes for chemosynthesis were examined: pmoA (encoding subunit A of the particulate methane monooxygenase) as an indicator...

  2. Microbial Communities and Chemosynthesis in Yellowstone Lake Sublacustrine Hydrothermal Vent Waters

    OpenAIRE

    Tingting eYang; Shawn eLyons; Carmen eAguilar; Russell eCuhel; Andreas eTeske

    2011-01-01

    Abstract. Five sublacustrine hydrothermal vent locations from 1-109 m water depth in Yellowstone Lake were surveyed by ribosomal RNA sequencing in relation to their chemical composition and dark CO2 fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16 - 110ºC) and electron donor supply (H2S <1 - >100µM; NH3 <0.5 - >10µM). Members of the Aquificales, most closely affiliated with the genus Sulfurihydrogenibium, are t...

  3. First Active Hydrothermal Vent Fields Discovered at the Equatorial Southern East Pacific Rise

    Science.gov (United States)

    Tao, C.; Lin, J.; Wu, G.; German, C. R.; Yoerger, D. R.; Chen, Y. J.; Guo, S.; Zeng, Z.; Han, X.; Zhou, N.; Li, J.; Xia, S.; Wang, H.; Ding, T.; Gao, S.; Qian, X.; Cui, R.; Zhou, J.; Ye, D.; Zhang8, Y.; Zhang, D.; Li, L.; Zhang, X.; Li, Y.; Wu, X.; Li, S.; He, Y.; Huang, W.; Wang, Y.; Wang, T.; Li, X.; Wang, K.; Gai, Y.; Science Party, D.; Baker, E. T.; Nakamura14, K.

    2008-12-01

    The third leg of the 2008 Chinese DY115-20 expedition on board R/V Dayangyihao has successfully discovered, for the first time, active hydrothermal vent fields on the fast-spreading Southern East Pacific Rise (SEPR) near the equator. This expedition follows the work of a 2005 expedition by R/V Dayangyihao, during which water column turbidity anomalies were measured in the region. The newly discovered vent fields are located along a 22-km-long ridge segment of the SEPR at 102.655°W/2.22°S, 102.646°W/2.152°S, 102.619°W/2.078°S, and 102.62°W/2.02°S, respectively, as well as on an off-axial volcano near 102.456°W/1.369°S. A significant portion of the activity appears to be concentrated along the edges of a seafloor fissure system. Furthermore, water column turbidity anomalies were observed over off-axis volcanoes near 102.827°W/2.084°S and 102.58°W/2.019°S. Video footage of the vent fields and water column turbidity, temperature, and methane anomalies were recorded by a deep-towed integrated system consisting of video, still camera, CTD, and ADCP, and MAPR and METS sensors. Two active hydrothermal fields at 2.217°S and 2.023°S were then extensively photographed and surveyed using the autonomous underwater vehicle ABE of the Woods Hole Oceanographic Institution (WHOI). Four samples of hydrothermal chimneys were successfully obtained by a TV-guided grab in three locations, showing evidence of high-temperature hydrothermal venting.

  4. The sound generated by mid-ocean ridge black smoker hydrothermal vents.

    Directory of Open Access Journals (Sweden)

    Timothy J Crone

    Full Text Available Hydrothermal flow through seafloor black smoker vents is typically turbulent and vigorous, with speeds often exceeding 1 m/s. Although theory predicts that these flows will generate sound, the prevailing view has been that black smokers are essentially silent. Here we present the first unambiguous field recordings showing that these vents radiate significant acoustic energy. The sounds contain a broadband component and narrowband tones which are indicative of resonance. The amplitude of the broadband component shows tidal modulation which is indicative of discharge rate variations related to the mechanics of tidal loading. Vent sounds will provide researchers with new ways to study flow through sulfide structures, and may provide some local organisms with behavioral or navigational cues.

  5. Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific.

    Directory of Open Access Journals (Sweden)

    Sayaka eMino

    2013-04-01

    Full Text Available Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephonella are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure such as intraspecific genomic diversity, distribution pattern, and phenotypic diversity. We developed the multi-locus sequence analysis (MLSA scheme for their genomic characterization. Sequence variation was determined in five housekeeping genes and one functional gene of 36 P. hydrogeniphila strains originated from the Okinawa Trough and the South Mariana Trough. Although the strains share > 98.7% similarities in 16S rRNA gene sequences, MLSA revealed 35 different sequence types, indicating their extensive genomic diversity. A phylogenetic tree inferred from all concatenated gene sequences revealed the clustering of isolates according to the geographic origin. In addition, the phenotypic clustering pattern inferred from whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS analysis can be correlated to their MLSA clustering pattern. This study represents the first MLSA combined with phenotypic analysis indicative of allopatric speciation of deep-sea hydrothermal vent bacteria.

  6. Mechatronic integration and implementation of in situ multipoint temperature measurement for seafloor hydrothermal vent

    Institute of Scientific and Technical Information of China (English)

    WU HuaiChao; CHEN Ying; YANG CanJun; ZHANG JiaFan; ZHOU HuaiYang; PENG XiaoTong; JI FuWu

    2007-01-01

    In order to provide firsthand reference data for model building and analysis of temperature field of seafloor hydrothermal vent, a temperature measurement system is designed, which can be used to measure the temperature of seafloor hydrothermal vent. The system can implement in situ multipoint temperature measurement and work for 15 days on the seefloor, so Iow power consumption design principle of the integrated circuit board is adopted. To enable the system to endure the high pressure on the seafloor, mechanical structure of the system is designed in terms of design principle of pressure container. The pressure test experiment was performed in the authoritative institution, and the results indicated that the system was safe and could work reliably on the seafloor. In the first Sino-American Joint Dive Cruise, the instruments were carried to the seafloor to work by Alvin. The experiment in the sea was successful, and the results indicated that the system could survive in the high pressure and high temperature environment and record the temperature activities of hydrothermal vents. About 710000 groups of temperature data were acquired, and these are of importance for further scientific researches.

  7. Mechatronic integration and implementation of in situ multipoint temperature measurement for seafloor hydrothermal vent

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    In order to provide firsthand reference data for model building and analysis of temperature field of seafloor hydrothermal vent, a temperature measurement sys- tem is designed, which can be used to measure the temperature of seafloor hydrothermal vent. The system can implement in situ multipoint temperature measurement and work for 15 days on the seafloor, so low power consumption design principle of the integrated circuit board is adopted. To enable the system to endure the high pressure on the seafloor, mechanical structure of the system is designed in terms of design principle of pressure container. The pressure test ex- periment was performed in the authoritative institution, and the results indicated that the system was safe and could work reliably on the seafloor. In the first Sino-American Joint Dive Cruise, the instruments were carried to the seafloor to work by Alvin. The experiment in the sea was successful, and the results indicated that the system could survive in the high pressure and high temperature environ- ment and record the temperature activities of hydrothermal vents. About 710000 groups of temperature data were acquired, and these are of importance for further scientific researches.

  8. Geothermic Potential Assessment of hydrothermal vents of Township Barranca De Upia - Meta - Colombia

    Science.gov (United States)

    Chica, J.; Chicangana, G.; Eco Energy Research Group

    2013-05-01

    Hydrothermal vents have been traditionally exploited in Colombia as a source of tourism revenue such as pools and saunas. Leaving aside its high potential for geothermal power generation in applications like heating, drying, cooling, extensive use in crops, livestock, electricity generation and more. Currently the use given to this natural resource in the town of Barranca de Upia in Meta department, central Colombia, is like Wellness Centre. However, the geothermal gradient for the area where hydrothermal vents occur, indicates that the water emerges at temperatures above 70 ° C (Alfaro et al., 2003), which opens a window of opportunity to assess their geothermal potential, in order to know the actual energy potential of the region as an option of augmenting their development. this research is the analysis of information gathered from databases in gravimetry and magnetometry of the study area and the temperatures measured in wells derived from the oil industry. Based on that information, a numerical analysis of the data will be performed in order to establish a model to parameterize the energy potential of the study area and identify possible uses of the energy contained by the hydrothermal vents.

  9. Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific.

    Science.gov (United States)

    Mino, Sayaka; Makita, Hiroko; Toki, Tomohiro; Miyazaki, Junichi; Kato, Shingo; Watanabe, Hiromi; Imachi, Hiroyuki; Watsuji, Tomo-O; Nunoura, Takuro; Kojima, Shigeaki; Sawabe, Tomoo; Takai, Ken; Nakagawa, Satoshi

    2013-01-01

    Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephonella are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure such as intraspecific genomic diversity, distribution pattern, and phenotypic diversity. We developed the multi-locus sequence analysis (MLSA) scheme for their genomic characterization. Sequence variation was determined in five housekeeping genes and one functional gene of 36 Persephonella hydrogeniphila strains originated from the Okinawa Trough and the South Mariana Trough (SNT). Although the strains share >98.7% similarities in 16S rRNA gene sequences, MLSA revealed 35 different sequence types (ST), indicating their extensive genomic diversity. A phylogenetic tree inferred from all concatenated gene sequences revealed the clustering of isolates according to the geographic origin. In addition, the phenotypic clustering pattern inferred from whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis can be correlated to their MLSA clustering pattern. This study represents the first MLSA combined with phenotypic analysis indicative of allopatric speciation of deep-sea hydrothermal vent bacteria. PMID:23630523

  10. Food Web Structure at South Su, Solwara 1 and Solwara 8 Hydrothermal Vent Sites (Manus Basin)

    Science.gov (United States)

    Honig, D. L.; Hsing, P.; Jones, R.; Schultz, T.; Sobel, A.; Thaler, A.; van Dover, C. L.

    2008-12-01

    A robust understanding of food webs in chemoautotrophically based hydrothermal vent ecosystems requires quantifying the input of local bacterial chemoautoptrophic production vs. photosynthetically derived debris from surface waters. As an initial step towards this goal for vent communities in Papua New Guinea's Manus Basin, we use isotopic ratios of carbon, nitrogen and sulfur to describe trophic relations among 17 invertebrate genera collected in July 2008 at the Solwara 1, Solwara 8 and South Su hydrothermal vent beds. Prior stable isotope work by Erickson, Macko and Van Dover (unpublished) at Manus Basin vent sites suggests that we will see relatively depleted ä13C and ä15N values for the primary consumers Ifremeria, Alviniconcha and Olgasolaris compared to secondary consumers like the mobile, scavenging genera Munidopsis, Austinograea, Alvinocaris and Chorocaris, sessile suspension feeders of the genera Eochinolasmus and Vulcanolepas, and the predatory sponge Abyssocladia. We further hypothesize that mobile fauna will exhibit greater within-genus variance of ä13C, ä15N and ä34S values than sessile genera due to mobile organisms' ability to forage for photosynthetically derived detritus.

  11. Vacuolate-attached filaments: highly productive Ridgeia piscesae epibionts at the Juan de Fuca hydrothermal vents.

    Science.gov (United States)

    Kalanetra, Karen M; Nelson, Douglas C

    2010-01-01

    Vacuolate sulfur bacteria with high morphological similarity to vacuolate-attached filaments previously described from shallow hydrothermal vents (White Point, CA) were found at deep-sea hydrothermal vents. These filamentous bacteria grow in dense mats that cover surfaces and potentially provide a significant source of organic carbon where they occur. Vacuolate-attached filaments were collected near vents at the Clam Bed site of the Endeavour Segment of the Juan de Fuca Ridge and from the sediment surface at Escanaba Trough on the Gorda Ridge. A phylogenetic analysis comparing their 16S rRNA gene sequences to those collected from the shallow White Point site showed that all vacuolate-attached filament sequences form a monophyletic group within the vacuolate sulfur-oxidizing bacteria clade in the gamma proteobacteria. Abundance of the attached filaments was quantified over the length of the exterior surface of the tubes of Ridgeia piscesae worms collected from the Clam Bed site at Juan de Fuca yielding a per worm average of 0.070 ± 0.018 cm(3) (n = 4). In agreement with previous results for White Point filaments, anion measurements by ion chromatography showed no detectable internal nitrate concentrations above ambient seawater (n = 9). For one R. piscesae tube worm "bush" at the Easter Island vent site, potential gross epibiont productivity is estimated to be 15 to 45× the net productivity of the worms. PMID:24391244

  12. Chemolithotrophic sulfur-oxidizing bacteria from the galapagos rift hydrothermal vents.

    Science.gov (United States)

    Ruby, E G; Wirsen, C O; Jannasch, H W

    1981-08-01

    Three distinct physiological types of sulfur-oxidizing bacteria were enriched and isolated from samples collected at several deep-sea hydrothermal vents (2,550 m) of the Galapagos Rift ocean floor spreading center. Twelve strains of the obligately chemolithotrophic genus Thiomicrospira were obtained from venting water and from microbial mats covering surfaces in the immediate vicinity of the vents. From these and other sources two types of obligately heterotrophic sulfur oxidizers were repeatedly isolated that presumably oxidized thiosulfate either to sulfate (acid producing; 9 strains) or to polythionates (base producing; 74 strains). The former were thiobacilli-like, exhibiting a thiosulfate-stimulated increase in growth and CO(2) incorporation, whereas the latter were similar to previously encountered pseudomonad-like heterotrophs. The presence of chemolithotrophic sulfur-oxidizing bacteria in the sulfide-containing hydrothermal water supports the hypothesis that chemosynthesis provides a substantial primary food source for the rich populations of invertebrates found in the immediate vicinity of the vents. PMID:16345831

  13. Phylogenetic diversity and functional gene patterns of sulfur-oxidizing subseafloor Epsilonproteobacteria in diffuse hydrothermal vent fluids

    OpenAIRE

    Akerman, Nancy H.; Butterfield, David A; Huber, Julie A.

    2013-01-01

    Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vents over a range of geochemical gradients from Axial Seamount, a deep-sea volcano in the Northeast Pac...

  14. Investigations of a novel fauna from hydrothermal vents along the Arctic Mid-Ocean Ridge (AMOR) (Invited)

    Science.gov (United States)

    Rapp, H.; Schander, C.; Halanych, K. M.; Levin, L. A.; Sweetman, A.; Tverberg, J.; Hoem, S.; Steen, I.; Thorseth, I. H.; Pedersen, R.

    2010-12-01

    The Arctic deep ocean hosts a variety of habitats ranging from fairly uniform sedimentary abyssal plains to highly variable hard bottoms on mid ocean ridges, including biodiversity hotspots like seamounts and hydrothermal vents. Deep-sea hydrothermal vents are usually associated with a highly specialized fauna, and since their discovery in 1977 more than 400 species of animals have been described. This fauna includes various animal groups of which the most conspicuous and well known are annelids, mollusks and crustaceans. The newly discovered deep sea hydrothermal vents on the Mohns-Knipovich ridge north of Iceland harbour unique biodiversity. The Jan Mayen field consists of two main areas with high-temperature white smoker venting and wide areas with low-temperature seepage, located at 5-700 m, while the deeper Loki Castle vent field at 2400 m depth consists of a large area with high temperature black smokers surrounded by a sedimentary area with more diffuse low-temperature venting and barite chimneys. The Jan Mayen sites show low abundance of specialized hydrothermal vent fauna. Single groups have a few specialized representatives but groups otherwise common in hydrothermal vent areas are absent. Slightly more than 200 macrofaunal species have been identified from this vent area, comprising mainly an assortment of bathyal species known from the surrounding area. Analysis of stable isotope data also indicates that the majority of the species present are feeding on phytodetritus and/or phytoplankton. However, the deeper Loki Castle vent field contains a much more diverse vent endemic fauna with high abundances of specialized polychaetes, gastropods and amphipods. These specializations also include symbioses with a range of chemosynthetic microorganisms. Our data show that the fauna composition is a result of high degree of local specialization with some similarities to the fauna of cold seeps along the Norwegian margin and wood-falls in the abyssal Norwegian Sea

  15. Liquid Carbon Dioxide Venting at the Champagne Hydrothermal Site, NW Eifuku Volcano, Mariana Arc

    Science.gov (United States)

    Lupton, J.; Lilley, M.; Butterfield, D.; Evans, L.; Embley, R.; Olson, E.; Proskurowski, G.; Resing, J.; Roe, K.; Greene, R.; Lebon, G.

    2004-12-01

    In March/April 2004, submersible dives with the remotely-operated vehicle ROPOS discovered an unusual CO2-rich hydrothermal system near the summit of NW Eifuku, a submarine volcano located at 21.49° N, 144.04° E in the northern Mariana Arc. Although several sites of hydrothermal discharge were located on NW Eifuku, the most intense venting was found at 1600-m depth at the Champagne site, slightly west of the volcano summit. The Champagne site was found to be discharging two distinct fluids into the ocean: a) several small white chimneys were emitting milky 103° C gas-rich hydrothermal fluid with at least millimolar levels of H2S and b) cold (grapes. The film coating the droplets was assumed to be CO2 hydrate (or clathrate) which is known to form whenever liquid CO2 contacts water under these P,T conditions. Samples of the 103° C hydrothermal fluids were collected in special gas-tight titanium sampling bottles that were able to withstand the high internal pressures created by the dissolved gases. The Champagne hydrothermal fluids contained a surprising 2.3 moles/kg of CO2, an order of magnitude higher than any CO2 values previously reported for submarine hydrothermal fluids. The overall gas composition was 87% CO2, yielded δ 13C = -1.75 ‰ , much heavier than the -6.0 ‰ typical for carbon in MOR vent fluids. The C/3He ratio was ~2.2 x 1010, an order of magnitude higher than the average value of 2 x 109 found in MOR vent fluids. The δ 13C and C/3He values suggest a substantial contribution to the carbon from subducted carbonates rather than mantle carbon. The Champagne site is only the second locality where liquid CO2 has been observed venting into the deep sea (the other reported location is in the Okinawa Trough, see Sakai et al., 1990). Because of the presence of liquid CO2 in proximity to hydrothermal organisms, the Champagne site may prove to be a valuable natural laboratory for studying the effects of high CO2 concentrations on marine ecosystems.

  16. Temporal and spatial variation in temperature experienced by macrofauna at Main Endeavour hydrothermal vent field

    Science.gov (United States)

    Lee, Raymond W.; Robert, Katleen; Matabos, Marjolaine; Bates, Amanda E.; Juniper, S. Kim

    2015-12-01

    A significant focus of hydrothermal vent ecological studies has been to understand how species cope with various stressors through physiological tolerance and biochemical resistance. Yet, the environmental conditions experienced by vent species have not been well characterized. This objective requires continuous observations over time intervals that can capture environmental variability at scales that are relevant to animals. We used autonomous temperature logger arrays (four roughly parallel linear arrays of 12 loggers spaced every 10-12 cm) to study spatial and temporal variations in the thermal regime experienced by hydrothermal vent macrofauna at a diffuse flow vent. Hourly temperatures were recorded over eight months from 2010 to 2011 at Grotto vent in the Main Endeavour vent field on the Juan de Fuca Ridge, a focus area of the Ocean Networks Canada cabled observatory. The conspicuous animal assemblages in video footage contained Ridgeia piscesae tubeworms, gastropods (primarily Lepetodrilus fucensis), and polychaetes (polynoid scaleworms and the palm worm Paralvinella palmiformis). Two dimensional spatial gradients in temperature were generally stable over the deployment period. The average temperature recorded by all arrays, and in some individual loggers, revealed distinctive fluctuations in temperature that often corresponded with the tidal cycle. We postulate that this may be related to changes in bottom currents or fluctuations in vent discharge. A marked transient temperature increase lasting over a period of days was observed in April 2011. While the distributions and behavior of Juan de Fuca Ridge vent invertebrates may be partially constrained by environmental temperature and temperature tolerance, except for the one transient high-temperature event, observed fluid temperatures were generally similar to the thermal preferences for some species, and typically well below lethal temperatures for all species. Average temperatures of the four arrays

  17. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Ji-Lu, E-mail: triace@163.com; Zhu, Ming-Qiang; Wu, Hai-tang

    2015-09-15

    Highlights: • Swine carcasses can be converted to bio-oil by alkaline hydrothermal liquefaction. • It seems that the use of the bio-oil for heat or CHP is technically suitable. • Some valuable chemicals were found in the bio-oils. • The bio-oil and the solid residue constituted an energy efficiency of 93.63% for the feedstock. • The solid residue can be used as a soil amendment, to sequester C and for preparing activated carbon. - Abstract: It is imperative that swine carcasses are disposed of safely, practically and economically. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil was performed. Firstly, the effects of temperature, reaction time and pH value on the yield of each liquefaction product were determined. Secondly, liquefaction products, including bio-oil and solid residue, were characterized. Finally, the energy recovery ratio (ERR), which was defined as the energy of the resultant products compared to the energy input of the material, was investigated. Our experiment shows that reaction time had certain influence on the yield of liquefaction products, but temperature and pH value had bigger influence on the yield of liquefaction products. Yields of 62.2 wt% bio-oil, having a high heating value of 32.35 MJ/kg and a viscosity of 305cp, and 22 wt% solid residue were realized at a liquefaction temperature of 250 °C, a reaction time of 60 min and a pH value of 9.0. The bio-oil contained up to hundreds of different chemical components that may be classified according to functional groups. Typical compound classes in the bio-oil were hydrocarbons, organic acids, esters, ketones and heterocyclics. The energy recovery ratio (ERR) reached 93.63%. The bio-oil is expected to contribute to fossil fuel replacement in stationary applications, including boilers and furnaces, and upgrading processes for the bio-oil may be used to obtain liquid transport fuels.

  18. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil

    International Nuclear Information System (INIS)

    Highlights: • Swine carcasses can be converted to bio-oil by alkaline hydrothermal liquefaction. • It seems that the use of the bio-oil for heat or CHP is technically suitable. • Some valuable chemicals were found in the bio-oils. • The bio-oil and the solid residue constituted an energy efficiency of 93.63% for the feedstock. • The solid residue can be used as a soil amendment, to sequester C and for preparing activated carbon. - Abstract: It is imperative that swine carcasses are disposed of safely, practically and economically. Alkaline hydrothermal liquefaction of swine carcasses to bio-oil was performed. Firstly, the effects of temperature, reaction time and pH value on the yield of each liquefaction product were determined. Secondly, liquefaction products, including bio-oil and solid residue, were characterized. Finally, the energy recovery ratio (ERR), which was defined as the energy of the resultant products compared to the energy input of the material, was investigated. Our experiment shows that reaction time had certain influence on the yield of liquefaction products, but temperature and pH value had bigger influence on the yield of liquefaction products. Yields of 62.2 wt% bio-oil, having a high heating value of 32.35 MJ/kg and a viscosity of 305cp, and 22 wt% solid residue were realized at a liquefaction temperature of 250 °C, a reaction time of 60 min and a pH value of 9.0. The bio-oil contained up to hundreds of different chemical components that may be classified according to functional groups. Typical compound classes in the bio-oil were hydrocarbons, organic acids, esters, ketones and heterocyclics. The energy recovery ratio (ERR) reached 93.63%. The bio-oil is expected to contribute to fossil fuel replacement in stationary applications, including boilers and furnaces, and upgrading processes for the bio-oil may be used to obtain liquid transport fuels

  19. Arsenic speciation in food chains from mid-Atlantic hydrothermal vents

    Science.gov (United States)

    Taylor, Vivien F.; Jackson, Brian P.; Siegfried, Matthew R.; Navratilova, Jana; Francesconi, Kevin A.; Kirshtein, Julie; Voytek, Mary

    2012-01-01

    Arsenic concentration and speciation were determined in benthic fauna collected from the Mid-Atlantic Ridge hydrothermal vents. The shrimp species, Rimicaris exoculata, the vent chimney-dwelling mussel, Bathymodiolus azoricus, Branchipolynoe seepensis, a commensal worm of B. azoricus and the gastropod Peltospira smaragdina showed variations in As concentration and in stable isotope (δ13C and δ15N) signature between species, suggesting different sources of As uptake. Arsenic speciation showed arsenobetaine to be the dominant species in R. exoculata, whereas in B. azoricus and B. seepensis arsenosugars were most abundant, although arsenobetaine, dimethylarsinate and inorganic arsenic were also observed, along with several unidentified species. Scrape samples from outside the vent chimneys covered with microbial mat, which is a presumed food source for many vent organisms, contained high levels of total As, but organic species were not detectable. The formation of arsenosugars in pelagic environments is typically attributed to marine algae, and the pathway to arsenobetaine is still unknown. The occurrence of arsenosugars and arsenobetaine in these deep sea organisms, where primary production is chemolithoautotrophic and stable isotope analyses indicate food sources are of vent origin, suggests that organic arsenicals can occur in a foodweb without algae or other photosynthetic life.

  20. Mesonerilla neridae, n. sp. (Nerillidae): First meiofaunal annelid from deep-sea hydrothermal vents

    DEFF Research Database (Denmark)

    Worsaae, Katrine; Rouse, Greg W

    2009-01-01

    deep-sea hydrothermal vent associated nerillid, this is the first record of an adult meiofaunal annelid from deep-sea hydrothermal vent areas and the first record of Mesonerilla from the deep sea. Based on the new material we here describe a new species of Mesonerilla, M. neridae sp. nov., with nine......) in 2005, six specimens of a new species of Mesonerilla were collected at depths of 2234-2649 m. Samples were taken via DSV Alvin with a slurp gun collecting fine silt and volcanic glass shards in cracks, fissures, and mussel beds from 5-20 m away from active venting areas. As well as being the first...

  1. Biodiversity and biogeography of hydrothermal vent species in the western Pacific: a biological perspective of TAIGA project

    Science.gov (United States)

    Seo, M.; Watanabe, H.; Nakamura, M.; Sasaki, T.; Ogura, T.; Yahagi, T.; Takahashi, Y.; Ishibashi, J.; Kojima, S.

    2012-12-01

    Deep-sea hydrothermal vents are scientifically interesting environments where strong interactions of geology, chemistry, and biology can be observed. The hydrothermal vents are geologically controlled in association with magmatic activities while diversity of chemicals (such as hydrogen sulfide) contained in hydrothermal fluid is controlled by geochemical interaction between heated seawater and surrounding rocks. In addition to those geological and chemical characters of hydrothermal vents, high biomasses of chemosynthetic community have been known around many vents since the first discovery in the 1970s. To understand the unique system and diversity of biological communities associated with vents is highly valuable in geological, chemical, and biological sciences. As an activity of the research project "TAIGA (Trans-crustal Advection & In-situ bio-geochemical processes of Global sub-seafloor Aquifer)" (Representative: Tetsuro Urabe, Department of Earth & Planetary Science, the University of Tokyo), we analyzed population structures and connectivity as well as larval ecology of various hydrothermal vent species in the Okinawa Trough and the Mariana Trough in an attempt to estimate faunal transitional history associated with hydrothermal activities. The specimens analyzed in the present study were collected by R/V Yokosuka with manned submersible Shinkai6500 and R/V Natsushima with ROV Hyper-Dolphin during YK10-11 and NT11-20 cruises, respectively. In the Mariana Trough (YK10-11), benthic and planktonic faunas were investigated by multiple sampling and use of plankton samplers in three hydrothermal vents (Snail, Archaean, and Urashima-Pika fields). Faunal compositions were then compared as well as size compositions and genetic diversities of major vent species among local populations. In the Okinawa Trough (NT11-20), multiple quantitative sampling was made with simultaneous environmental measurements at more than two sites in five hydrothermal vents (Minami

  2. Vibrio diabolicus challenge in Bathymodiolus azoricus populations from Menez Gwen and Lucky Strike hydrothermal vent sites.

    Science.gov (United States)

    Martins, Eva; Santos, Ricardo Serrão; Bettencourt, Raul

    2015-12-01

    Menez Gwen (MG) and Lucky Strike (LS) deep-sea hydrothermal vents are located at 850 m and 1730 m depths respectively and support chemosynthesis-based ecosystems partially differing in heavy metal concentration, temperature range, and faunistic composition. The successfully adapted deep-sea vent mussel Bathymodiolus azoricus is found at both vent locations. In such inhospitable environments survival strategies rely on the establishment of bacteria-vent animal symbiosis In spite of the toxic nature of deep-sea vents, the problem of microbial threat and the need for immunity exist in B. azoricus. This study aims at investigating the immune system of B. azoricus from MG and LS populations by comparing immune gene expressions profiles using the deep-sea vent-related Vibrio diabolicus. Expression of nineteen immune genes was analyzed from gill, digestive gland and mantle tissues upon 3 h, 12 h and 24 h V. diabolicus challenges. Based on quantitative-Polymerase Chain Reaction (qPCR) significant gene expression differences were found among MG and LS populations and challenge times MG mussels revealed that gill and digestive gland gene expression levels were remarkably higher than those from LS mussels. Expression of Carcinolectin, Serpin-2, SRCR, IRGs, RTK, TLR2, NF-κB, HSP70 and Ferritin genes was greater in MG than LS mussels. In contrast, mantle tissue from LS mussels revealed the highest peak of expression at 24 h for most genes analyzed. The activation of immune signaling pathways demonstrated that gene expression profiles are distinct between the two mussel populations. These differences may possibly ensue from intrinsic immune transcriptional activities upon which host responses are modulated in presence of V. diabolicus. mRNA transcript variations were assessed during 24 h acclimatization taking into account the partial depuration to which mussels were subjected to. Additionally, gene expression differences may reflect still accountable effects from the presence

  3. Discovery of New Hydrothermal Venting Sites in the Lau Basin, Tonga Back Arc

    Science.gov (United States)

    Crowhurst, P. V.; Arculus, R. J.; Massoth, G. J.; Baptista, L.; Stevenson, I.; Angus, R.; Baker, E. T.; Walker, S. L.; Nakamura, K.

    2009-12-01

    Between 22 April and 25 June 2009, a systematic search for hydrothermal venting along 1340 km of back-arc features was conducted throughout the Lau Basin aboard the CSIRO owned RV Southern Surveyor. The selection of survey areas was based on bathymetry, sidescan and water column anomaly datasets collected during previous marine science research and commercial exploration voyages. During 54 operational days, 76 CTD tows were completed using real-time plume mapping protocols, augmented with mini autonomous plume recorders, to discern anomalies in light scattering, and oxidation-reduction potential with water samples collected within the peak anomalies. Coincident with CTD towing at an average speed of 1.1 knots high resolution EM300 bathymetry and backscatter data was collected which significantly enhanced geological interpretation of possible source sites for follow up cross tows. 32 venting sites were detected, 24 of which are believed to be new discoveries. 13 dredge operations were conducted on 7 of these sites. Sulfides were recovered from 2 sites, one being a new discovery on the NE Lau spreading centre, ~14 km north of the commercial discovery by Teck and ~7km north of the eruption site discovery during a RV Thompson NOAA survey, both during 2008. The new venting field discoveries at North Mata, northern extent of the CLSC and far southern Valu Fa ridge are beyond any previously known areas of hydrothermal activity and further enhances the reputation of the Lau Basin as one of the most productive back arc regions for hydrothermally active spreading centers. A significant number of filter residue samples collected from the vent sites yielded greater than background values for metals including Cu and Zn, which is interpreted to imply they were sourced from active seafloor massive sulfide systems rather than volcanic activity.

  4. Investigation of extractable organic compounds in deep-sea hydrothermal vent fluids along the Mid-Atlantic Ridge

    Science.gov (United States)

    McCollom, Thomas M.; Seewald, Jeffrey S.; German, Christopher R.

    2015-05-01

    The possibility that deep-sea hydrothermal vents may contain organic compounds produced by abiotic synthesis or by microbial communities living deep beneath the surface has led to numerous studies of the organic composition of vent fluids. Most of these studies have focused on methane and other light hydrocarbons, while the possible occurrence of more complex organic compounds in the fluids has remained largely unstudied. To address this issue, the presence of higher molecular weight organic compounds in deep-sea hydrothermal fluids was assessed at three sites along the Mid-Atlantic Ridge that span a range of temperatures (51 to >360 °C), fluid compositions, and host-rock lithologies (mafic to ultramafic). Samples were obtained at several sites within the Lucky Strike, Rainbow, and Lost City hydrothermal fields. Three methods were employed to extract organic compounds for analysis, including liquid:liquid extraction, cold trapping on the walls of a coil of titanium tubing, and pumping fluids through cartridges filled with solid phase extraction (SPE) sorbents. The only samples to consistently yield high amounts of extractable organic compounds were the warm (51-91 °C), highly alkaline fluids from Lost City, which contained elevated concentrations of C8, C10, and C12n-alkanoic acids and, in some cases, trithiolane, hexadecanol, squalene, and cholesterol. Collectively, the C8-C12 acids can account for about 15% of the total dissolved organic carbon in the Lost City fluids. The even-carbon-number predominance of the alkanoic acids indicates a biological origin, but it is unclear whether these compounds are derived from microbial activity occurring within the hydrothermal chimney proximal to the site of fluid discharge or are transported from deeper within the system. Hydrothermal fluids from the Lucky Strike and Rainbow fields were characterized by an overall scarcity of extractable dissolved organic compounds. Trace amounts of aromatic hydrocarbons including

  5. High levels of natural radioactivity in biota from deep sea hydrothermal vents

    International Nuclear Information System (INIS)

    Hydrothermal vent ecosystems are associated with areas of tectonic activities throughout the deep sea and are thus enriched in natural primitive radionuclides characterizing the magma source i.e. uranium-thorium series. However, the amount of data on radionuclide content in hydrothermal vent biota is very scarce. Here we present data from various archived biological samples collected on several hydrothermal vent site. Samples were collected by manned or unmanned submersibles on the East Pacific Rise (EPR) in 1996 and 2002 and on the Mid Atlantic Ridge (MAR) in 2001. Their concentrations in uranium and thorium isotopes were determined by isotope dilution mass spectrometry. Measurements were performed with a Sector field ICP-MS, the Axiom single collector from VG Elemental (Winsford, Cheshire, UK). 210Po was determined through alpha spectrometry. Results in Table II underline that high levels in uranium isotopes are found in polychaetes from the East Pacific Rise. The highest contents characterize their tubes plus mucus samples. On the contrary, the deep-sea amphipod sample (Orchomenella) collected outside hydrothermal vent areas exhibits the lowest values though direct comparison is difficult due to variability between species. 238U contents in coastal marine organisms are generally in the range 1 to 5 Bq kg-1 dry weight. Comparison between sites (Atlantic vs Pacific) is not obvious since different species have been sampled on MAR and EPR but the highest levels characterize the samples from EPR. Some samples demonstrate 234U/238U ratios very close to the mean value for seawater (1.1 - 1.2) but four present lower ratios (i.e. one sample of Bathymodiolus, Paralvinella, Riftia and Orchomenella). This is certainly to be linked to an uptake of particulate uranium. 210Po contents are very high in polychaetes tubes and mucus and are almost entirely supported by 210Pb. This in agreement with what has been reported by Cherry et al. (1992) for polychaetes (Alvinella and

  6. Behaviors of 210Pb and 210Po around hydrothermal vents in the Okinawa Trough

    Institute of Scientific and Technical Information of China (English)

    杨永亮; 日下部正志; 山本惠幸

    2002-01-01

    Vertical profiles for the uranium-series radioisotopes 210Pb and 210Po were obtained at the two hydrothermal vent sites, the Iheya Ridge and the Minami-Ensei Knoll, in the Mid-Okinawa Trough in 1993 and 1994, respectively. In 1995, both radioisotopes were measured at the Minami-Ensei Knoll again. At the Iheya Ridge, where the hydrothermal activity is not active as reflected by the CH4 and 222Rn data, both the total 210Pb and 210Po activities show deficiency relative to their parents, and the mean residence time of 210Pb and 210Po is approximately equal to 20 and 2~ 5 a, respectively. At the Minami-Ensei Knoll, which is characterized by black smokers, the total 210Pb(0.167 × 10-3 ~2.5 × 10-3 Bq/kg) around the plumes is deficient relative to 226Ra but the total 210Po activities ( 1.83 × 10- 3 ~ 2.83× 10-3 Bq/kg) are in excess relative to 210Pb. The 210Po activities are higher than those in the East China Sea and the Okinawa Trough and excess 210Po has been found. The 210Pb/226Ra and 210Po/210Pb activity ratios are 0.1~ 0.4 and 1.1 ~ 7.8, respectively. 210Pb is preferentially scavenged in the hydrothermal plumes. The possible mechanisms governing the concentration of 210Pb in the Minami-Ensei Knoll involve a lateral transport of 210Pb via diffusion. The high 210Po/210Pb activity ratios in the hydrothermal plumes suggest a depletion of 210Pb and addition of 210Po in the hydrothermal vent area.

  7. Anaerobic respiration on tellurate and other metalloids in bacteria from hydrothermal vent fields in the eastern Pacific Ocean.

    Science.gov (United States)

    Csotonyi, Julius T; Stackebrandt, Erko; Yurkov, Vladimir

    2006-07-01

    This paper reports the discovery of anaerobic respiration on tellurate by bacteria isolated from deep ocean (1,543 to 1,791 m) hydrothermal vent worms. The first evidence for selenite- and vanadate-respiring bacteria from deep ocean hydrothermal vents is also presented. Enumeration of the anaerobic metal(loid)-resistant microbial community associated with hydrothermal vent animals indicates that a greater proportion of the bacterial community associated with certain vent fauna resists and reduces metal(loid)s anaerobically than aerobically, suggesting that anaerobic metal(loid) respiration might be an important process in bacteria that are symbiotic with vent fauna. Isolates from Axial Volcano and Explorer Ridge were tested for their ability to reduce tellurate, selenite, metavanadate, or orthovanadate in the absence of alternate electron acceptors. In the presence of metal(loid)s, strains showed an ability to grow and produce ATP, whereas in the absence of metal(loid)s, no growth or ATP production was observed. The protonophore carbonyl cyanide m-chlorophenylhydrazone depressed metal(loid) reduction. Anaerobic tellurate respiration will be a significant component in describing biogeochemical cycling of Te at hydrothermal vents. PMID:16820492

  8. Bacterial and archaeal communities in the deep-sea sediments of inactive hydrothermal vents in the Southwest India Ridge.

    Science.gov (United States)

    Zhang, Likui; Kang, Manyu; Xu, Jiajun; Xu, Jian; Shuai, Yinjie; Zhou, Xiaojian; Yang, Zhihui; Ma, Kesen

    2016-01-01

    Active deep-sea hydrothermal vents harbor abundant thermophilic and hyperthermophilic microorganisms. However, microbial communities in inactive hydrothermal vents have not been well documented. Here, we investigated bacterial and archaeal communities in the two deep-sea sediments (named as TVG4 and TVG11) collected from inactive hydrothermal vents in the Southwest India Ridge using the high-throughput sequencing technology of Illumina MiSeq2500 platform. Based on the V4 region of 16S rRNA gene, sequence analysis showed that bacterial communities in the two samples were dominated by Proteobacteria, followed by Bacteroidetes, Actinobacteria and Firmicutes. Furthermore, archaeal communities in the two samples were dominated by Thaumarchaeota and Euryarchaeota. Comparative analysis showed that (i) TVG4 displayed the higher bacterial richness and lower archaeal richness than TVG11; (ii) the two samples had more divergence in archaeal communities than bacterial communities. Bacteria and archaea that are potentially associated with nitrogen, sulfur metal and methane cycling were detected in the two samples. Overall, we first provided a comparative picture of bacterial and archaeal communities and revealed their potentially ecological roles in the deep-sea environments of inactive hydrothermal vents in the Southwest Indian Ridge, augmenting microbial communities in inactive hydrothermal vents. PMID:27169490

  9. Microbial diversity of a sulfide black smoker in main endeavour hydrothermal vent field, Juan de Fuca Ridge.

    Science.gov (United States)

    Zhou, Huaiyang; Li, Jiangtao; Peng, Xiaotong; Meng, Jun; Wang, Fengping; Ai, Yuncan

    2009-06-01

    Submarine hydrothermal vents are among the least-understood habitats on Earth but have been the intense focus of research in the past 30 years. An active hydrothermal sulfide chimney collected from the Dudley site in the Main Endeavour vent Field (MEF) of Juan de Fuca Ridge was investigated using mineralogical and molecular approaches. Mineral analysis indicated that the chimney was composed mainly of Fe-, Zn-and Cu-rich sulfides. According to phylogenetic analysis, within the Crenarchaeota, clones of the order Desulfurococcales predominated, comprising nearly 50% of archaeal clones. Euryarchaeota were composed mainly of clones belonging to Thermococcales and deep-sea hydrothermal vent Euryarchaeota (DHVE), each of which accounted for about 20% of all clones. Thermophilic or hyperthermophilic physiologies were common to the predominant archaeal groups. More than half of bacterial clones belonged to epsilon-Proteobacteria, which confirmed their prevalence in hydrothermal vent environments. Clones of Proteobacteria (gamma-, delta-, beta-), Cytophaga-Flavobacterium-Bacteroides (CFB) and Deinococcus-Thermus occurred as well. It was remarkable that methanogens and methanotrophs were not detected in our 16S rRNA gene library. Our results indicated that sulfur-related metabolism, which included sulfur-reducing activity carried out by thermophilic archaea and sulfur-oxidizing by mesophilic bacteria, was common and crucial to the vent ecosystem in Dudley hydrothermal site. PMID:19557339

  10. Magnetic Structure of Backarc Spreading Axis with Hydrothermal Vents; the Southern Mariana Trough

    Science.gov (United States)

    Fujii, M.; Okino, K.; Mochizuki, N.; Honsho, C.; Szitkar, F.; Dyment, J.; Nakamura, K.

    2012-12-01

    Seafloor hydrothermal systems are important in relation to global heat and chemical fluxes as well as habitat of microbial communities. The substantial variation of hydrothermal systems in various tectonic settings has important implications for the magnetic structure of oceanic crust. It has been very difficult to detect the geophysical signature of hydrothermal systems from sea-surface data because the small scale of hydrothermal systems is below the limit of resolution. The advance of near-bottom survey methods using a submersible, deep-tow, ROV and AUV has made possible high-resolution geophysical mapping around hydrothermal areas. Near-bottom magnetic surveys can provide direct information on the magnetization of the shallower oceanic crust, implying hydrothermal alteration both in active and fossil vent sites. Near-bottom three component magnetic measurements on submersible Shinkai 6500 were carried out at hydrothermal fields in the Southern Mariana Trough, a slow spreading backarc basin. Fourteen dive surveys were conducted during cruises YK11-10 and YK10-11. We investigated the magnetic structure of four hydrothermal systems located at on- and off-axis to clarify how the geophysical and geological setting controls the fluid circulation at small scale. Recent researches at slow spreading ridges showed a relationship between crustal magnetic structure and host rock around hydrothermal vents (e.g. Tivey and Dyment, 2010), but no observation at backarc spreading axis has been reported so far. We carefully corrected the effects of induced and permanent magnetizations of the submersible by applying the method of Isezaki [1986] with dumped least-square method (Honsho et al., 2009). After subtracting the IGRF from the corrected observed data, we obtained geomagnetic vector anomalies in geographical coordinate. For three transects of the axis, we applied three methods; 2D inversion technique (Parker and Huestis, 1972), 2D forward modeling technique (Honsho et al

  11. Microbial Primary Productivity in Hydrothermal Vent Chimneys at Middle Valley, Juan de Fuca Ridge

    Science.gov (United States)

    Olins, H. C.; Rogers, D.; Frank, K. L.; Girguis, P. R.; Vidoudez, C.

    2012-12-01

    Chemosynthetic primary productivity supports hydrothermal vent ecosystems, but the extent of that productivity has not been well measured. To examine the role that environmental temperature plays in controlling carbon fixation rates, and to assess the degree to which microbial community composition, in situ geochemistry, and mineralogy influence carbon fixation, we conducted a series of shipboard incubations across a range of temperatures (4, 25, 50 and 90°C) and at environmentally relevant geochemical conditions using material recovered from three hydrothermal vent chimneys in the Middle Valley hydrothermal vent field (Juan de Fuca Ridge). Net rates of carbon fixation (CFX) were greatest at lower temperatures, and were similar among structures. Rates did not correlate with the mineralogy or the geochemical composition of the high temperature fluids at each chimney. No obvious patterns of association were observed between carbon fixation rates and microbial community composition. Abundance of selected functional genes related to different carbon fixation pathway exhibited striking differences among the three study sites, but did not correlate with rates. Natural carbon isotope ratios implicate the Calvin Benson Bassham Cycle as the dominant mechanism of primary production in these systems, despite the abundance of genes related to other pathways (and presumably some degree of activity). Together these data reveal that primary productivity by endolithic communities does not exhibit much variation among these chimneys, and further reveal that microbial activity cannot easily be related to mineralogical and geochemical assessments that are made at a coarser scale. Indeed, the relationships between carbon fixation rates and community composition/functional gene abundance were also likely obfuscated by differences in scale at which these measurements were made. Regardless, these data reveal the degree to which endolithic, anaerobic carbon fixation contributes to

  12. Hydrogen-limited growth of hyperthermophilic methanogens at deep-sea hydrothermal vents.

    Science.gov (United States)

    Ver Eecke, Helene C; Butterfield, David A; Huber, Julie A; Lilley, Marvin D; Olson, Eric J; Roe, Kevin K; Evans, Leigh J; Merkel, Alexandr Y; Cantin, Holly V; Holden, James F

    2012-08-21

    Microbial productivity at hydrothermal vents is among the highest found anywhere in the deep ocean, but constraints on microbial growth and metabolism at vents are lacking. We used a combination of cultivation, molecular, and geochemical tools to verify pure culture H(2) threshold measurements for hyperthermophilic methanogenesis in low-temperature hydrothermal fluids from Axial Volcano and Endeavour Segment in the northeastern Pacific Ocean. Two Methanocaldococcus strains from Axial and Methanocaldococcus jannaschii showed similar Monod growth kinetics when grown in a bioreactor at varying H(2) concentrations. Their H(2) half-saturation value was 66 μM, and growth ceased below 17-23 μM H(2), 10-fold lower than previously predicted. By comparison, measured H(2) and CH(4) concentrations in fluids suggest that there was generally sufficient H(2) for Methanocaldococcus growth at Axial but not at Endeavour. Fluids from one vent at Axial (Marker 113) had anomalously high CH(4) concentrations and contained various thermal classes of methanogens based on cultivation and mcrA/mrtA analyses. At Endeavour, methanogens were largely undetectable in fluid samples based on cultivation and molecular screens, although abundances of hyperthermophilic heterotrophs were relatively high. Where present, Methanocaldococcus genes were the predominant mcrA/mrtA sequences recovered and comprised ∼0.2-6% of the total archaeal community. Field and coculture data suggest that H(2) limitation may be partly ameliorated by H(2) syntrophy with hyperthermophilic heterotrophs. These data support our estimated H(2) threshold for hyperthermophilic methanogenesis at vents and highlight the need for coupled laboratory and field measurements to constrain microbial distribution and biogeochemical impacts in the deep sea. PMID:22869718

  13. Fossils of hydrothermal vent worms from Cretaceous sulfide ores of the Samail ophiolite, Oman

    Science.gov (United States)

    Haymon, R.M.; Koski, R.A.; Sinclair, C.

    1984-01-01

    Fossil worm tubes of Cretaceous age preserved in the Bayda massive sulfide deposit of the Samail ophiolite, Oman, are apparently the first documented examples of fossils embedded in massive sulfide deposits from the geologic record. The geologic setting of the Bayda deposit and the distinctive mineralogic and textural features of the fossiliferous samples suggest that the Bayda sulfide deposit and fossil fauna are remnants of a Cretaceous sea-floor hydrothermal vent similar to modern hot springs on the East Pacific Rise and the Juan de Fuca Ridge.

  14. Bacterial Group II Introns in a Deep-Sea Hydrothermal Vent Environment†

    OpenAIRE

    Podar, Mircea; Mullineaux, Lauren; Huang, Hon-Ren; Perlman, Philip S.; Sogin, Mitchell L.

    2002-01-01

    Group II introns are catalytic RNAs and mobile retrotransposable elements known to be present in the genomes of some nonmarine bacteria and eukaryotic organelles. Here we report the discovery of group II introns in a bacterial mat sample collected from a deep-sea hydrothermal vent near 9°N on the East Pacific Rise. One of the introns was shown to self-splice in vitro. This is the first example of marine bacterial introns from molecular population structure studies of microorganisms that live ...

  15. Detection of methane plumes in the water column of Logatchev hydrothermal vent field, Mid-Atlantic Ridge

    Institute of Scientific and Technical Information of China (English)

    ZHOU HuaiYang; WU ZiJun; PENG XiaoTong; JIANG Lei; TANG Song

    2007-01-01

    During DY105-17 cruise onboard the R/V "Da Yang Yi Hao" in 2005, methane concentrations in the water column above Logachev hydrothermal vent field were measured by applying stripping/trapping-gas chromatographic (GC) and the distinct methane plumes were detected. Results show that the background methane concentration within the Logachev area is from 1.05 nmol/L to 1.68 nmol/L, significantly higher than the background level of the Atlantic abyssal plain of 0.4-0.5 nmol/L, suggesting that hydrothermal venting is a major source of dissolved methane to the ocean. The highest anomalies of methane concentrations in the water column range from 7.14 nmol/L to 113.9 nmol/L and occur just at 180-500 m above the seafloor. The distribution of methane concentration and the structural characteristics of hydrothermal plumes are strongly influenced by the supply of underlying hydrothermal fluids, the mixing process of ocean bottom currents and the microbial oxidation. Furthermore, the differences in distribution of methane plume between the station MAR-CTD3 and the other stations indicate a probable unknown hydrothermal vent site nearby. There occurs high concentration of methane along with temperature and nephelometry anomalies, which strongly confirms that the subtle measurement of methane concentration in water column is one of the effective ways to locate active sites of hydrothermal venting.

  16. The influence of vent fluid chemistry on trophic structure at two deep-sea hydrothermal vent fields on the Mid-Cayman Rise

    Science.gov (United States)

    Bennett, Sarah; Van Dover, Cindy; Coleman, Max

    2014-05-01

    The two known deep-sea hydrothermal vent fields along the Mid-Cayman Rise are separated by a distance of only 21 km, yet their chemistry and faunal diversity are distinct. The deeper of the two vent fields, Piccard (with active venting from Beebe Vents, Beebe Woods and Beebe Sea), at 4980 m is basalt hosted. The shallower vent field, Von Damm, at 2300 m appears to have an ultramafic influence. The Von Damm vent field can be separated into two sites: The Spire and The Tubeworm Field. The dominant vent fluids at the Tubeworm Field are distinct from those at the Spire, as a result of fluid modification in the sub-surface. Von Damm and Piccard vent fields support abundant invertebrates, sharing the same biomass-dominant shrimp species, Rimicaris hybisae. Although there are some other shared species (squat lobsters (Munidopsis sp.) and gastropods (Provanna sp. and Iheyaspira sp.)) between the vent fields, they are much more abundant at one site than the other. In this study we have examined the bulk carbon, nitrogen and sulfur isotope composition of microbes and fauna at each vent field. With these data we have deduced the trophic structure of the communities and the influence of vent fluid chemistry. From stable isotope data and end-member vent fluid chemistry, we infer that the basis of the trophic structure at Piccard is dominated by sulfur, iron, and hydrogen-oxidizing microbial communities. In comparison, the basis of the Von Damm trophic structure is dominated by microbial communities of sulfur and hydrogen oxidizers, sulfate reducers and methanotrophs. This microbial diversity at the base of the trophic structure is a result of chemical variations in vent fluids and processes in the sub-surface that alter the vent fluid chemistry. These differences influence higher trophic levels and can be used to explain some of the variability as well as similarity in fauna at the vent sites. Part of this work was performed at the Jet Propulsion Laboratory, California

  17. Hydrothermal vents near a mantle hot spot: the Lucky Strike vent field at 37°N on the Mid-Atlantic Ridge

    Science.gov (United States)

    Langmuir, C.; Humphris, S.; Fornari, D.; Van Dover, C.; Von Damm, K.; Tivey, M. K.; Colodner, D.; Charlou, J.-L.; Desonie, D.; Wilson, C.; Fouquet, Y.; Klinkhammer, G.; Bougault, H.

    1997-04-01

    The Lucky Strike hydrothermal field occurs in the summit basin of a large seamount that forms the shallow center of a 65 km long ridge segment near 37°N on the Mid-Atlantic Ridge. The depth and chemistry of the ridge segment are influenced by the Azores hot spot, and this hydrothermal field is the first Atlantic site found on crust that is dominated by a hot spot signature. Multiple hydrothermal vents occur over an area of at least 300 m by 700 m. Vent morphologies range from flanges and chimneys with temperatures of 200-212°C, to black smoker chimneys with temperatures up to 333°C. Cooler fluids from northern vents have higher chlorinities and lower gas volumes, while hotter, southern fluids have chlorinities 20% below seawater with higher gas volumes, suggesting phase separation has influenced their compositions. All gas volumes in fluids are higher than those at TAG and Snake Pit hydrothermal fields. Black smokers exhibit their typical mineralogy, except that barite is a major mineral, particularly at lower-temperature sites, which contrasts with previously investigated Atlantic sites. The fluid chemistry, distribution of the relict sulfide deposits on the seamount summit in the areas investigated using DSV Alvin, and contact relationships between active vent sites and surrounding basaltic and sulfide substrate suggest that the hydrothermal system has a long history and may have recently been rejuvenated. Fauna at the Lucky Strike vent sites are dominated by a new species of mussel, and include the first reported sea urchins. The Lucky Strike biological community differs considerably from other vent fauna at the species level and appears to be a new biogeographic province. The Lucky Strike field helps to constrain how variations in the basaltic substrate influence the composition of hydrothermal fluids and solids, because basalt compositions at Lucky Strike are 10-30 times enriched in incompatible elements compared to other Atlantic hydrothermal sites such as

  18. Stable-Carbon-Isotope Composition of Fatty Acids in Hydrothermal Vent Mussels Containing Methanotrophic and Thiotrophic Bacterial Endosymbionts

    OpenAIRE

    Pond, David W; Bell, Michael V; Dixon, David R.; Fallick, Anthony E.; Segonzac, Michel; Sargent, John R.

    1998-01-01

    Fatty acid biomarker analysis coupled with gas chromatography-isotope ratio mass spectrometry was used to confirm the presence of methanotrophic and thiotrophic bacterial endosymbionts in the tissues of a hydrothermal vent mussel (Bathymodiolus sp.), collected from the Menez Gwen vent field on the mid-Atlantic ridge. Monounsaturated (n-8) fatty acids, which are diagnostic of methanotrophic bacteria, were detected in all three types of tissues examined (gill, posterior adductor, and mantle), a...

  19. Culturable bacterial phylogeny from a shallow water hydrothermal vent of Espalamaca (Faial, Azores) reveals a variety of novel taxa

    Digital Repository Service at National Institute of Oceanography (India)

    Rajasabapathy, R.; Mohandass, C.; Colaco, A.; Dastager, S.G.; Santos, R.S.; Meena, R.M.

    ). Addition of metals in the isolation media will attract the metal-resistant bacteria since the hydrothermal vent regions are rich in various metals and elements. At the same time growth of the bacteria should not be hampered by low or high concentrations... relationships of representative novel taxa obtained from vent and nonvent regions of Espalamaca based on neighbour- joining analysis of 16S rRNA gene sequences data. The percentage of replicate trees in which the associated taxa clustered together...

  20. The dynamics of mid-ocean ridge hydrothermal systems: Splitting plumes and fluctuating vent temperatures

    Science.gov (United States)

    Coumou, Dim; Driesner, Thomas; Geiger, Sebastian; Heinrich, Christoph A.; Matthäi, Stephan

    2006-05-01

    We present new, accurate numerical simulations of 2D models resembling hydrothermal systems active in the high-permeability axial plane of mid-ocean ridges and show that fluid flow patterns are much more irregular and convection much more unstable than reported in previous simulation studies. First, we observe the splitting of hot, rising plumes. This phenomenon is caused by the viscous instability at the interface between hot, low-viscosity fluid and cold, high-viscosity fluid. This process, known as Taylor-Saffman fingering could potentially explain the sudden extinguishing of black smokers. Second, our simulations show that for relatively moderate permeabilities, convection is unsteady resulting in transiently varying vent temperatures. The amplitude of these fluctuations typically is 40 °C with a period of decades or less, depending on the permeability. Although externally imposed events such as dike injections are possible mechanisms, they are not required to explain temperature variations observed in natural systems. Our results also offer a simple explanation of how seismic events cause fluctuating temperatures: Earthquake-induced permeability-increase shifts the hydrothermal system to the unsteady regime with accompanying fluctuating vent temperatures. We demonstrate that realistic modelling of these high-Rayleigh number convection systems does not only require the use of real fluid properties, but also the use of higher order numerical methods capable of handling high-resolution meshes. Less accurate numerical solutions smear out sharp advection fronts and thereby artificially stabilize the system.

  1. The spatial scale of genetic subdivision in populations of Ifremeria nautilei, a hydrothermal-vent gastropod from the southwest Pacific

    Directory of Open Access Journals (Sweden)

    Thaler Andrew D

    2011-12-01

    Full Text Available Abstract Background Deep-sea hydrothermal vents provide patchy, ephemeral habitats for specialized communities of animals that depend on chemoautotrophic primary production. Unlike eastern Pacific hydrothermal vents, where population structure has been studied at large (thousands of kilometres and small (hundreds of meters spatial scales, population structure of western Pacific vents has received limited attention. This study addresses the scale at which genetic differentiation occurs among populations of a western Pacific vent-restricted gastropod, Ifremeria nautilei. Results We used mitochondrial and DNA microsatellite markers to infer patterns of gene flow and population subdivision. A nested sampling strategy was employed to compare genetic diversity in discrete patches of Ifremeria nautilei separated by a few meters within a single vent field to distances as great as several thousand kilometres between back-arc basins that encompass the known range of the species. No genetic subdivisions were detected among patches, mounds, or sites within Manus Basin. Although I. nautilei from Lau and North Fiji Basins (~1000 km apart also exhibited no evidence for genetic subdivision, these populations were genetically distinct from the Manus Basin population. Conclusions An unknown process that restricts contemporary gene flow isolates the Manus Basin population of Ifremeria nautilei from widespread populations that occupy the North Fiji and Lau Basins. A robust understanding of the genetic structure of hydrothermal vent populations at multiple spatial scales defines natural conservation units and can help minimize loss of genetic diversity in situations where human activities are proposed and managed.

  2. Intergradation between discrete lineages of Tevnia jerichonana, a deep-sea hydrothermal vent tubeworm

    Science.gov (United States)

    Zhang, Haibin; Johnson, Shannon B.; Flores, Vanessa R.; Vrijenhoek, Robert C.

    2015-11-01

    We describe a broad zone of intergradation between genetically differentiated, northern and southern lineages of the hydrothermal vent tubeworm, Tevnia jerichonana. DNA sequences from four genes, nuclear HSP and ATPsα and mitochondrial COI and Cytb were examined in samples from eastern Pacific vent localities between 13°N and 38°S latitude. Allelic frequencies at these loci exhibited concordant latitudinal clines, and genetic differentiation (pairwise ΦST's) increased with geographical distances between sample localities. Though this pattern of differentiation suggested isolation-by-distance (IBD), it appeared to result from hierarchical population structure. Genotypic assignment tests identified two population clusters comprised of samples from the northern East Pacific Rise (NEPR: 9-13°N) and an extension of the Pacific-Antarctic Ridge (PAR: 31-32°S) with a zone of intergradation along the southern East Pacific Rise (SEPR: 7-17°S). The overall degrees of DNA sequence divergence between the NEPR and PAR populations were slight and not indicative of lengthy isolation. Bayesian assignment methods suggested that the SEPR populations constitute intergrades that connect the NEPR and PAR populations. Though it typically is difficult to distinguish between primary and secondary intergradation, our results were consistent with parallel studies of vent-restricted species that suggest a high degree of demographic instability along the superfast-spreading SEPR axis. Frequent local extinctions and immigration from NEPR and PAR refugia probably shaped the observed pattern of intergradation.

  3. Genetic diversity and demographic instability in Riftia pachyptila tubeworms from eastern Pacific hydrothermal vents

    Science.gov (United States)

    Coykendall, D.K.; Johnson, S.B.; Karl, S.A.; Lutz, R.A.; Vrijenhoek, R.C.

    2011-01-01

    Background: Deep-sea hydrothermal vent animals occupy patchy and ephemeral habitats supported by chemosynthetic primary production. Volcanic and tectonic activities controlling the turnover of these habitats contribute to demographic instability that erodes genetic variation within and among colonies of these animals. We examined DNA sequences from one mitochondrial and three nuclear gene loci to assess genetic diversity in the siboglinid tubeworm, Riftia pachyptila, a widely distributed constituent of vents along the East Pacific Rise and Galpagos Rift. Results: Genetic differentiation (FST) among populations increased with geographical distances, as expected under a linear stepping-stone model of dispersal. Low levels of DNA sequence diversity occurred at all four loci, allowing us to exclude the hypothesis that an idiosyncratic selective sweep eliminated mitochondrial diversity alone. Total gene diversity declined with tectonic spreading rates. The southernmost populations, which are subjected to superfast spreading rates and high probabilities of extinction, are relatively homogenous genetically. Conclusions: Compared to other vent species, DNA sequence diversity is extremely low in R. pachyptila. Though its dispersal abilities appear to be effective, the low diversity, particularly in southern hemisphere populations, is consistent with frequent local extinction and (re)colonization events. ?? 2011 Coykendall et al; licensee BioMed Central Ltd.

  4. Subsurface conditions in hydrothermal vents inferred from diffuse flow composition, and models of reaction and transport

    Science.gov (United States)

    Larson, B. I.; Houghton, J. L.; Lowell, R. P.; Farough, A.; Meile, C. D.

    2015-08-01

    Chemical gradients in the subsurface of mid-ocean ridge hydrothermal systems create an environment where minerals precipitate and dissolve and where chemosynthetic organisms thrive. However, owing to the lack of easy access to the subsurface, robust knowledge of the nature and extent of chemical transformations remains elusive. Here, we combine measurements of vent fluid chemistry with geochemical and transport modeling to give new insights into the under-sampled subsurface. Temperature-composition relationships from a geochemical mixing model are superimposed on the subsurface temperature distribution determined using a heat flow model to estimate the spatial distribution of fluid composition. We then estimate the distribution of Gibb's free energies of reaction beneath mid oceanic ridges and by combining flow simulations with speciation calculations estimate anhydrite deposition rates. Applied to vent endmembers observed at the fast spreading ridge at the East Pacific Rise, our results suggest that sealing times due to anhydrite formation are longer than the typical time between tectonic and magmatic events. The chemical composition of the neighboring low temperature flow indicates relatively uniform energetically favorable conditions for commonly inferred microbial processes such as methanogenesis, sulfate reduction and numerous oxidation reactions, suggesting that factors other than energy availability may control subsurface microbial biomass distribution. Thus, these model simulations complement fluid-sample datasets from surface venting and help infer the chemical distribution and transformations in subsurface flow.

  5. Genetic diversity and demographic instability in Riftia pachyptila tubeworms from eastern Pacific hydrothermal vents

    Directory of Open Access Journals (Sweden)

    Lutz Richard A

    2011-04-01

    Full Text Available Abstract Background Deep-sea hydrothermal vent animals occupy patchy and ephemeral habitats supported by chemosynthetic primary production. Volcanic and tectonic activities controlling the turnover of these habitats contribute to demographic instability that erodes genetic variation within and among colonies of these animals. We examined DNA sequences from one mitochondrial and three nuclear gene loci to assess genetic diversity in the siboglinid tubeworm, Riftia pachyptila, a widely distributed constituent of vents along the East Pacific Rise and Galápagos Rift. Results Genetic differentiation (FST among populations increased with geographical distances, as expected under a linear stepping-stone model of dispersal. Low levels of DNA sequence diversity occurred at all four loci, allowing us to exclude the hypothesis that an idiosyncratic selective sweep eliminated mitochondrial diversity alone. Total gene diversity declined with tectonic spreading rates. The southernmost populations, which are subjected to superfast spreading rates and high probabilities of extinction, are relatively homogenous genetically. Conclusions Compared to other vent species, DNA sequence diversity is extremely low in R. pachyptila. Though its dispersal abilities appear to be effective, the low diversity, particularly in southern hemisphere populations, is consistent with frequent local extinction and (recolonization events.

  6. Microbial communities and chemosynthesis in Yellowstone Lake sublacustrine hydrothermal vent waters

    Directory of Open Access Journals (Sweden)

    Tingting eYang

    2011-06-01

    Full Text Available Abstract. Five sublacustrine hydrothermal vent locations from 1-109 m water depth in Yellowstone Lake were surveyed by ribosomal RNA sequencing in relation to their chemical composition and dark CO2 fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16 - 110ºC and electron donor supply (H2S <1 - >100µM; NH3 <0.5 - >10µM. Members of the Aquificales, most closely affiliated with the genus Sulfurihydrogenibium, are the most frequently recovered bacterial 16S rRNA gene phylotypes in the hottest samples; the detection of these thermophilic sulfur-oxidizing autotrophs coincided with maximal dark CO2 fixation rates reaching near 9 µM C h-1 at temperatures of 50 to 60°C. Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga. In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution.

  7. Hydrothermal vent gastropods from the same family (Provannidae) harbour epsilon- and gamma-proteobacterial endosymbionts.

    Science.gov (United States)

    Urakawa, Hidetoshi; Dubilier, Nicole; Fujiwara, Yoshihiro; Cunningham, Dale E; Kojima, Shigeaki; Stahl, David A

    2005-05-01

    The discovery of new hydrothermal vent systems in the back-arc basins of the Western Pacific revealed chemosynthesis-based faunal communities distinct from those of other vents. These vents are dominated by two related gastropods (Alviniconcha spp. and Ifremeria nautilei) that harbour symbiotic bacteria in their gills. We used comparative 16S ribosomal RNA (rRNA) gene sequencing and in situ hybridization with rRNA-targeted probes to characterize the bacterial symbionts of Alviniconcha sp. and I. nautilei from the Manus Basin in the Western Pacific. The analyses revealed that these two gastropod species, although affiliated with the same family, harbour phylogenetically distant chemosymbionts, suggesting independent origins of these endosymbioses. The I. nautilei endosymbiont clusters with sulfur-oxidizing bacteria within the gamma-Proteobacteria, as is the case for all previously characterized endosymbionts from a wide diversity of host taxa harbouring thioautotrophic prokaryotes. In contrast, the Alviniconcha endosymbiont is affiliated with sulfur-oxidizing bacteria within the epsilon-Proteobacteria. These results show that bacteria from the epsilon-Proteobacteria are also capable of forming endosymbiotic associations with marine invertebrates from chemosynthetic environments. More generally, the endosymbiotic lifestyle is now shown to be distributed throughout all recognized classes of the Proteobacteria. PMID:15819856

  8. Hydrothermal flow at Main Endeavour Field imaged and measured with Cable Operated Vent Imaging Sonar

    Science.gov (United States)

    Rona, P. A.; Bemis, K. G.; Xu, G.; Jackson, D. R.; Jones, C. D.

    2011-12-01

    Initial acoustic monitoring of hydrothermal flow in the Main Endeavour Field (MEF) captures the spatial distribution of diffuse and focused discharge and shows potential for flux determinations. Our Cabled Observatory Vent Imaging Sonar (COVIS) was connected to the NEPTUNE Canada Endeavour Observatory in September 2010. Using a customized Reson 7125 multi-beam sonar, COVIS acquired a 29 day time series of black smoker plume and associated diffuse hydrothermal flow from Grotto, a 30 m diameter vent cluster in the MEF, Juan de Fuca Ridge. Detection of the spatial patterns of diffuse flow utilizes phase decorrelation of the acoustic signal (200kHz) by buoyancy-driven turbulence (acoustic scintillation) to produce a time series of maps. Substantial fluctuation in the detected diffuse flow area (0.1 - 18 m^2) was observed over the 29 days of observation, although position remained stable. Acoustic imaging of focused flow (400 kHz) utilizes high volume backscatter (attributed to particles and turbulent sound speed fluctuations) to image in 3D the initial tens of meters of rise of buoyant plumes. Spectral analysis of bending inclination of a strong plume from multiple fast smokers on the NW end of Grotto (north tower) indicates that the dominant modes correspond with the ambient mixed semi-diurnal tide (based on current meter data at a mooring 2.9 km to the north and on a tidal model), with at least one secondary mode attributable to sub-inertial flow related to inflow to the axial valley. A weaker plume from several slower smokers is present on the NE end of Grotto. On first analysis, the bending inclination of the weaker plume appears to be affected by the stronger plume. Quantification of flow velocity and volume flux of plumes begins with measuring the Doppler phase shift through plume cross-sections beginning at 5 m above source vents where discharge merges. The volume flux measurements enable calculation of entrainment coefficients, which prior work on the same

  9. Characterizing Microbial Community and Geochemical Dynamics at Hydrothermal Vents Using Osmotically Driven Continuous Fluid Samplers

    Energy Technology Data Exchange (ETDEWEB)

    Robidart, Julie C.; Callister, Stephen J.; Song, Peng F.; Nicora, Carrie D.; Wheat, Charles G.; Girguis, Peter R.

    2013-05-07

    Microbes play a key role in mediating all aquatic biogeochemical cycles, and ongoing efforts are aimed at better understanding the relationships between microbial phylogenetic and physiological diversity, and habitat physical and chemical characteristics. Establishing such relationships is facilitated by sampling and studying microbiology and geochemistry at the appropriate spatial and temporal scales, to access information on the past and current environmental state that contributes to observed microbial abundances and activities. A modest number of sampling systems exist to date, few of which can be used in remote, harsh environments such as hydrothermal vents, where the ephemeral nature of venting underscores the necessity for higher resolution sampling. We have developed a robust, continuous fluid sampling system for co-registered microbial and biogeochemical analyses. The osmosis-powered bio-osmosampling system (BOSS) use no electricity, collects fluids with daily resolution or better, can be deployed in harsh, inaccessible environments and can sample fluids continuously for up to five years. Here we present a series of tests to examine DNA, RNA and protein stability over time, as well as material compatability, via lab experiments. We also conducted two field deployments at deep-sea hydrothermal vents to assess changes in microbial diversity and protein expression as a function of the physico-chemical environment. Our data reveal significant changes in microbial community composition co-occurring with relatively modest changes in the geochemistry. These data additionally provide new insights into the distribution of an enigmatic sulfur oxidizing symbiont in its free-living state. Data from the second deployment reveal differences in the representation of peptides over time, underscoring the utility of the BOSS in meta-proteomic studies. In concert, these data demonstrate the efficacy of this approach, and illustrate the value of using this method to study

  10. New insights into hydrothermal vent processes in the unique shallow-submarine arc-volcano, Kolumbo (Santorini), Greece

    OpenAIRE

    Kilias, Stephanos P.; Nomikou, Paraskevi; Papanikolaou, Dimitrios; Polymenakou, Paraskevi N.; Godelitsas, Athanasios; Argyraki, Ariadne; Carey, Steven; Gamaletsos, Platon; Mertzimekis, Theo J.; Stathopoulou, Eleni; Goettlicher, Joerg; Steininger, Ralph; Betzelou, Konstantina; Livanos, Isidoros; Christakis, Christos

    2013-01-01

    We report on integrated geomorphological, mineralogical, geochemical and biological investigations of the hydrothermal vent field located on the floor of the density-stratified acidic (pH ~ 5) crater of the Kolumbo shallow-submarine arc-volcano, near Santorini. Kolumbo features rare geodynamic setting at convergent boundaries, where arc-volcanism and seafloor hydrothermal activity are occurring in thinned continental crust. Special focus is given to unique enrichments of polymetallic spires i...

  11. Phylogenetic diversity and functional gene patterns of sulfur-oxidizing subseafloor Epsilonproteobacteria in diffuse hydrothermal vent fluids.

    Science.gov (United States)

    Akerman, Nancy H; Butterfield, David A; Huber, Julie A

    2013-01-01

    Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vents over a range of geochemical gradients from Axial Seamount, a deep-sea volcano in the Northeast Pacific. PCR primers were developed and applied to target the sulfur oxidation soxB gene of Epsilonproteobacteria. soxB genes belonging to the genera Sulfurimonas and Sulfurovum are both present and expressed at most diffuse vent sites, but not in background seawater. Although Sulfurovum-like soxB genes were detected in all fluid samples, the RNA profiles were nearly identical among the vents and suggest that Sulfurimonas-like species are the primary Epsilonproteobacteria responsible for actively oxidizing sulfur via the Sox pathway at each vent. Community patterns of subseafloor Epsilonproteobacteria 16S rRNA genes were best matched to methane concentrations in vent fluids, as well as individual vent locations, indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations. The data show that in the subseafloor at Axial Seamount, Epsilonproteobacteria are expressing the soxB gene and that microbial patterns in community distribution are linked to both vent location and chemistry. PMID:23847608

  12. Phylogenetic diversity and functional gene patterns of sulfur-oxidizing subseafloor Epsilonproteobacteria in diffuse hydrothermal vent fluids

    Directory of Open Access Journals (Sweden)

    JulieAHuber

    2013-07-01

    Full Text Available Microorganisms throughout the dark ocean use reduced sulfur compounds for chemolithoautotrophy. In many deep-sea hydrothermal vents, sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism both at and beneath the seafloor. In this study, the presence and activity of vent endemic Epsilonproteobacteria was examined in six low-temperature diffuse vents over a range of geochemical gradients from Axial Seamount, a deep-sea volcano in the Northeast Pacific. PCR primers were developed and applied to target the sulfur oxidation soxB gene of Epsilonproteobacteria. soxB genes belonging to the genera Sulfurimonas and Sulfurovum are both present and expressed at most diffuse vent sites, but not in background seawater. Although Sulfurovum-like soxB genes were detected in all fluid samples, the RNA profiles were nearly identical among the vents and suggest that Sulfurimonas-like species are the primary Epsilonproteobacteria responsible for actively oxidizing sulfur via the Sox pathway at each vent. Community patterns of subseafloor Epsilonproteobacteria 16S rRNA genes were best matched to methane concentrations in vent fluids, as well as individual vent locations, indicating that both geochemistry and geographical isolation play a role in structuring subseafloor microbial populations. The data show that in the subseafloor at Axial Seamount, Epsilonproteobacteria are expressing the soxB gene and that microbial patterns in community distribution are linked to both vent location and chemistry.

  13. Assessing the influence of physical, geochemical and biological factors on anaerobic microbial primary productivity within hydrothermal vent chimneys.

    Science.gov (United States)

    Olins, H C; Rogers, D R; Frank, K L; Vidoudez, C; Girguis, P R

    2013-05-01

    Chemosynthetic primary production supports hydrothermal vent ecosystems, but the extent of that productivity and its governing factors have not been well constrained. To better understand anaerobic primary production within massive vent deposits, we conducted a series of incubations at 4, 25, 50 and 90 °C using aggregates recovered from hydrothermal vent structures. We documented in situ geochemistry, measured autochthonous organic carbon stable isotope ratios and assessed microbial community composition and functional gene abundances in three hydrothermal vent chimney structures from Middle Valley on the Juan de Fuca Ridge. Carbon fixation rates were greatest at lower temperatures and were comparable among chimneys. Stable isotope ratios of autochthonous organic carbon were consistent with the Calvin-Benson-Bassham cycle being the predominant mode of carbon fixation for all three chimneys. Chimneys exhibited marked differences in vent fluid geochemistry and microbial community composition, with structures being differentially dominated by gamma (γ) or epsilon (ε) proteobacteria. Similarly, qPCR analyses of functional genes representing different carbon fixation pathways showed striking differences in gene abundance among chimney structures. Carbon fixation rates showed no obvious correlation with observed in situ vent fluid geochemistry, community composition or functional gene abundance. Together, these data reveal that (i) net anaerobic carbon fixation rates among these chimneys are elevated at lower temperatures, (ii) clear differences in community composition and gene abundance exist among chimney structures, and (iii) tremendous spatial heterogeneity within these environments likely confounds efforts to relate the observed rates to in situ microbial and geochemical factors. We also posit that microbes typically thought to be mesophiles are likely active and growing at cooler temperatures, and that their activity at these temperatures comprises the

  14. Precipitation and growth of barite within hydrothermal vent deposits from the Endeavour Segment, Juan de Fuca Ridge

    Science.gov (United States)

    Jamieson, John William; Hannington, Mark D.; Tivey, Margaret K.; Hansteen, Thor; Williamson, Nicole M.-B.; Stewart, Margaret; Fietzke, Jan; Butterfield, David; Frische, Matthias; Allen, Leigh; Cousens, Brian; Langer, Julia

    2016-01-01

    Hydrothermal vent deposits form on the seafloor as a result of cooling and mixing of hot hydrothermal fluids with cold seawater. Amongst the major sulfide and sulfate minerals that are preserved at vent sites, barite (BaSO4) is unique because it requires the direct mixing of Ba-rich hydrothermal fluid with sulfate-rich seawater in order for precipitation to occur. Because of its extremely low solubility, barite crystals preserve geochemical fingerprints associated with conditions of formation. Here, we present data from petrographic and geochemical analyses of hydrothermal barite from the Endeavour Segment of the Juan de Fuca Ridge, northeast Pacific Ocean, in order to determine the physical and chemical conditions under which barite precipitates within seafloor hydrothermal vent systems. Petrographic analyses of 22 barite-rich samples show a range of barite crystal morphologies: dendritic and acicular barite forms near the exterior vent walls, whereas larger bladed and tabular crystals occur within the interior of chimneys. A two component mixing model based on Sr concentrations and 87Sr/86Sr of both seawater and hydrothermal fluid, combined with 87Sr/86Sr data from whole rock and laser-ablation ICP-MS analyses of barite crystals indicate that barite precipitates from mixtures containing as low as 17% and as high as 88% hydrothermal fluid component, relative to seawater. Geochemical modelling of the relationship between aqueous species concentrations and degree of fluid mixing indicates that Ba2+ availability is the dominant control on mineral saturation. Observations combined with model results support that dendritic barite forms from fluids of less than 40% hydrothermal component and with a saturation index greater than ∼0.6, whereas more euhedral crystals form at lower levels of supersaturation associated with greater contributions of hydrothermal fluid. Fluid inclusions within barite indicate formation temperatures of between ∼120 °C and 240 °C during

  15. A Retrievable Mineral Microcosm for Examining Microbial Colonization and Mineral Precipitation at Seafloor Hydrothermal Vents

    Science.gov (United States)

    Dunn, E. E.; Holloway, J. R.; Cary, S.; Voglesonger, K. M.; Ashbridge, D. A.; O'Day, P. A.

    2002-12-01

    Although seafloor hydrothermal vent environments are known to support thriving ecosystems, the microscale physical and chemical environment suitable for microbial colonization and the identity of pioneering organisms is unknown. Because of the fragility of young chimneys and their ephemeral nature, novel methods for sample retrieval and analysis are required. The mineral microcosm consists of four titanium mesh chambers containing crushed minerals mounted on a titanium base that allows for fluid flow through the chambers. The chambers can be filled with different minerals or mineral mixtures (or no minerals) to supply different substrates for microbial colonization and different local microenvironments as minerals react with the surrounding fluids. The device sets on top of an active hydrothermal vent for a period of days to weeks to allow colonization and mineral reaction. The mineral microcosm was deployed during the Atlantis/Alvin Extreme 2001 Cruise (Oct.- Nov.,2001) to 9° 50'N on the East Pacific Rise a total of three times, for ~ 24, ~ 96, and ~ 48 hours each. It was deployed in two different environments, twice in lower temperature (350°C).Seed minerals included sulfides, sulfates, magnetite, apatite, and quartz, both individually and in mixtures. In the first 24-hour deployment, dissolution of anhydrite but not sulfide minerals within the chambers indicated high temperatures in chamber interiors and rapid reaction rates. Temperatures measured on chamber exteriors before retrieval ranged from 4° -98°C. The 96-hour deployment on a hot vent (fluid ~370°C before deployment) resulted in extensive mineral precipitation and chimney growth inside the mineral chambers, on the outer surfaces of the chambers, and on the platform as a whole, creating micro-chimneys several centimeters tall. The young chimneys were mainly composed of pyrite with lesser amounts of chalcopyrite and sphalerite and with thin veneers of anhydrite on exterior surfaces in contact with

  16. Phylogenetic Diversity of Nitrogenase (nifH) Genes in Deep-Sea and Hydrothermal Vent Environments of the Juan de Fuca Ridge

    OpenAIRE

    Mehta, Mausmi P.; David A Butterfield; Baross, John A.

    2003-01-01

    The subseafloor microbial habitat associated with typical unsedimented mid-ocean-ridge hydrothermal vent ecosystems may be limited by the availability of fixed nitrogen, inferred by the low ammonium and nitrate concentrations measured in diffuse hydrothermal fluid. Dissolved N2 gas, the largest reservoir of nitrogen in the ocean, is abundant in deep-sea and hydrothermal vent fluid. In order to test the hypothesis that biological nitrogen fixation plays an important role in nitrogen cycling in...

  17. Discovery of Nascent Vents and Recent Colonization Associated with(Re)activated Hydrothermal Vent Fields by the GALREX 2011 Expedition on the Galápagos Rift

    Science.gov (United States)

    Shank, T. M.; Holden, J. F.; Herrera, S.; Munro, C.; Muric, T.; Lin, J.; Stuart, L.

    2011-12-01

    GALREX 2011 was a NOAA OER telepresence cruise that explored the diverse habitats and geologic settings of the deep Galápagos region. The expedition made12 Little Hercules ROV dives in July 2011.Abundant corals and a strong depth zonation of species (including deepwater coral communities) were found near 500 m depth on Paramount Seamount, likely influenced by past low sea level states, wave-cut terrace processes, and the historical presence of shallow reef structures. At fresh lava flows with associated (flocculent) hydrothermal venting near 88° W, now known as Uka Pacha and Pegasus Vent Fields, rocks were coated with white microbial mat and lacked sessile fauna, with few mobile fauna (e.g., bythograeid crabs, alvinocarid shrimp, polynoid worms, zoarcid fish, and dirivultid copepods). This suggests a recent creation of hydrothermal habitats through volcanic eruptions and/or diking events, which may have taken place over a 15 km span separating the two vent fields. The Rosebud vent field at 86°W was not observed and may have been covered with lava since last visited in 2005. A hydrothermal vent field near 86°W was discovered that is one of the largest vent fields known on the Rift (120m by 40m). Low-temperature vent habitats were colonized by low numbers of tubeworms including Riftia, Oasisia, and a potential Tevnia species (the latter not previously observed on the Galapagos Rift). Patches of tubeworms were observed with individuals less than 2cm in length, and the relatively few large Riftia had tube lengths near 70cm long. Large numbers of small (microbial communities were observed on the underside and vertical surfaces of basalt rock surfaces. There were at least 13 species of vent-endemic fauna. The active colonization was observed on relatively older basalt pillows and lobate lavas ringed by and amidst a large dead bed of Calyptogena clams (most with broken and dissolving shells greater than 25 cm in length, with a few of the same size living amongst the

  18. In situ Raman-based detections of the hydrothermal vent and cold seep fluids

    Science.gov (United States)

    Zhang, Xin; Du, Zengfeng; Zheng, Ronger; Luan, Zhendong; Qi, Fujun; Cheng, Kai; Wang, Bing; Ye, Wangquan; Liu, Xiaorui; Chen, Changan; Guo, Jinjia; Li, Ying; Yan, Jun

    2016-04-01

    Hydrothermal vents and cold seeps, and their associated biological communities play an important role in global carbon and sulphur biogeochemical cycles. Most of the studies of fluid composition geochemistry are based on recovered samples, both with gas-tight samplers and as open specimens, but the in situ conditions are difficult to maintain in recovered samples. Determination in situ of the chemical signals of the emerging fluids are challenging due to the high pressure, often strongly acidic and temperature in which few sensors can survive. Most of those sensors used so far are based on electrochemistry, and can typically detect only a few chemical species. Here we show that direct measurement of critical chemical species of hydrothermal vents and cold seeps can be made rapidly and in situ by means of a new hybrid version of earlier deep-sea pore water Raman probe carried on the ROV (Remote Operated Vehicle) Faxian. The fluid was drawn through the probe by actuating a hydraulic pump on the ROV, and measured at the probe optical cell through a sapphire window. We have observed the concentrations of H2S, HS‑, SO42‑, HSO4‑, CO2, and H2 in hydrothermal vent fluids from the Pacmanus and Desmos vent systems in the Manus back-arc basin, Papua New Guinea. Two black smokers (279° C and 186° C) at the Pacmanus site showed the characteristic loss of SO42‑, and the increase of CO2 and well resolved H2S and HS‑ peaks. At the white smoker of Onsen site the strong HSO4‑peak observed at high temperature quickly dropped with strong accompanying increase of SO42‑and H2 peaks when the sample contained in the Raman sensing cell was removed from the hot fluid due to rapid thermal deprotonation. We report here also the finding of a new lower temperature (88° C) white smoker "Kexue" field at the Desmos site with strong H2S, HS‑ and CO2 signals. We also have detected the concentrations of CH4,H2S, HS‑, SO42‑, and S8 in cold seep fluids and the surrounding

  19. Major transitions in evolution linked to thermal gradients above hydrothermal vents

    CERN Document Server

    Muller, Anthonie W J

    2012-01-01

    The emergence of the main divisions of today's life: (1) unicellular prokaryotes, (2) unicellular eukaryotes, (3) multicellular eukaryotes, and (4) metazoans, are examples of the--still unexplained--major transitions in evolution. Regarding the origin of life, I have proposed that primordial life functioned as heat engine (thermosynthesis) while thermally cycled in convecting volcanic hot springs. Here I argue for a role of thermal gradients above submarine hydrothermal vents (SHV) in several major transitions. The last decade has witnessed the emergence of phononics, a novel discipline in physics based on controlled heat transport in thermal gradients. It builds thermal analogs to electronic devices: the thermal diode, the thermal transistor, the thermal switch, the thermal amplifier, the thermal memory--the thermal computer has been proposed. Encouraged by (1) the many similarities between microtubules (MT) and carbon nanotubes, which have a very high thermal conductivity, and (2) the recent discovery of a ...

  20. Trace Metal and Sulfur Dynamics in the First Meter of Buoyant Hydrothermal Vent Plumes

    Science.gov (United States)

    Findlay, A.; Gartman, A.; Shaw, T. J.; Luther, G. W., III

    2014-12-01

    The speciation and reactivity of metals and metal sulfides within the buoyant plume is critical to determining the ultimate fate of metals emitted from hydrothermal vents. The concentration, size fractionation, and partitioning of trace metals (Fe, Mn, Cu, Co, Zn, Cd, Pb) were determined within the first meter of the rising plume at three vent fields (TAG, Snakepit, and Rainbow) along the Mid-Atlantic Ridge. At Rainbow, total Fe concentrations exceed total sulfide concentrations by an order of magnitude, whereas at the other two sites, total Fe and total sulfide concentrations are nearly equal. At all three sites, Mn and Fe are primarily in the filtered (zinc is correlated with unfiltered cadmium and lead. At Rainbow, unfiltered zinc, cadmium and lead are correlated, but unfiltered copper and cobalt are not, indicating precipitation dynamics at Rainbow are different than those at TAG and Snakepit due to bulk geochemical differences, including a higher iron to sulfide ratio. A sequential HCl/HNO3 leaching method was used to distinguish metals present in pyrite and chalcopyrite in both unfiltered and filtered samples. Significant portions of unfiltered Cu and Co were extracted in HNO3, whereas unfiltered Zn, Cd, and Pb were extracted in HCl. Up to 95 % of filtered Cu, Co, and Zn, up to 80% Cd, and up to 60 % Pb are only extractable in HNO3, indicating that a significant portion of metals < 0.2 μm are incorporated into a recalcitrant fraction such as nanoparticulate pyrite or chalcopyrite.

  1. Novel forms of structural integration between microbes and a hydrothermal vent gastropod from the Indian Ocean.

    Science.gov (United States)

    Goffredi, Shana K; Warén, Anders; Orphan, Victoria J; Van Dover, Cindy L; Vrijenhoek, Robert C

    2004-05-01

    Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of gamma-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting differs from that of all other vent mollusks, which harbor sulfur-oxidizing endosymbionts in their gills. The significantly reduced digestive tract, the isotopic signatures of the snail tissues, and the presence of internal bacteria suggest a dependence on chemoautotrophy for nutrition. Most notably, this snail is unique in having a dense coat of mineralized scales covering the sides of its foot, a feature seen in no other living metazoan. The scales are coated with iron sulfides (pyrite and greigite) and heavily colonized by epsilon- and delta-proteobacteria, likely participating in mineralization of the sclerites. This novel metazoan-microbial collaboration illustrates the great potential of organismal adaptation in chemically and physically challenging deep-sea environments. PMID:15128570

  2. Photoprotective Bioactivity Present in a Unique Marine Bacteria Collection from Portuguese Deep Sea Hydrothermal Vents

    Directory of Open Access Journals (Sweden)

    Patrícia Calado

    2013-05-01

    Full Text Available Interesting biological activities have been found for numerous marine compounds. In fact, screening of phylogenetically diverse marine microorganisms from extreme environments revealed to be a rational approach for the discovery of novel molecules with relevant bioactivities for industries such as pharmaceutical and cosmeceutical. Nevertheless, marine sources deliverables are still far from the expectations and new extreme sources of microbes should be explored. In this work, a marine prokaryotic collection from four Mid-Atlantic Ridge (MAR deep sea hydrothermal vents near the Azores Islands, Portugal, was created, characterized and tested for its photoprotective capacity. Within 246 isolates, a polyphasic approach, using chemotaxonomic and molecular typing methods, identified 23-related clusters of phenetically similar isolates with high indexes of diversity. Interestingly, 16S rRNA gene sequencing suggested the presence of 43% new prokaryotic species. A sub-set of 139 isolates of the prokaryotic collection was selected for biotechnological exploitation with 484 bacterial extracts prepared in a sustainable upscalling manner. 22% of the extracts showed an industrially relevant photoprotective activity, with two extracts, belonging to new strains of the species Shewanella algae and Vibrio fluvialis, uniquely showing UV-A, UV-B and UV-C protective capacity. This clearly demonstrates the high potential of the bacteria MAR vents collection in natural product synthesis with market applications.

  3. Linking geology, fluid chemistry, and microbial activity of basalt- and ultramafic-hosted deep-sea hydrothermal vent environments.

    Science.gov (United States)

    Perner, M; Hansen, M; Seifert, R; Strauss, H; Koschinsky, A; Petersen, S

    2013-07-01

    Hydrothermal fluids passing through basaltic rocks along mid-ocean ridges are known to be enriched in sulfide, while those circulating through ultramafic mantle rocks are typically elevated in hydrogen. Therefore, it has been estimated that the maximum energy in basalt-hosted systems is available through sulfide oxidation and in ultramafic-hosted systems through hydrogen oxidation. Furthermore, thermodynamic models suggest that the greatest biomass potential arises from sulfide oxidation in basalt-hosted and from hydrogen oxidation in ultramafic-hosted systems. We tested these predictions by measuring biological sulfide and hydrogen removal and subsequent autotrophic CO2 fixation in chemically distinct hydrothermal fluids from basalt-hosted and ultramafic-hosted vents. We found a large potential of microbial hydrogen oxidation in naturally hydrogen-rich (ultramafic-hosted) but also in naturally hydrogen-poor (basalt-hosted) hydrothermal fluids. Moreover, hydrogen oxidation-based primary production proved to be highly attractive under our incubation conditions regardless whether hydrothermal fluids from ultramafic-hosted or basalt-hosted sites were used. Site-specific hydrogen and sulfide availability alone did not appear to determine whether hydrogen or sulfide oxidation provides the energy for primary production by the free-living microbes in the tested hydrothermal fluids. This suggests that more complex features (e.g., a combination of oxygen, temperature, biological interactions) may play a role for determining which energy source is preferably used in chemically distinct hydrothermal vent biotopes. PMID:23647923

  4. Event Detection for Hydrothermal Plumes: A case study at Grotto Vent

    Science.gov (United States)

    Bemis, K. G.; Ozer, S.; Xu, G.; Rona, P. A.; Silver, D.

    2012-12-01

    Evidence is mounting that geologic events such as volcanic eruptions (and intrusions) and earthquakes (near and far) influence the flow rates and temperatures of hydrothermal systems. Connecting such suppositions to observations of hydrothermal output is challenging, but new ongoing time series have the potential to capture such events. This study explores using activity detection, a technique modified from computer vision, to identify pre-defined events within an extended time series recorded by COVIS (Cabled Observatory Vent Imaging Sonar) and applies it to a time series, with gaps, from Sept 2010 to the present; available measurements include plume orientation, plume rise rate, and diffuse flow area at the NEPTUNE Canada Observatory at Grotto Vent, Main Endeavour Field, Juan de Fuca Ridge. Activity detection is the process of finding a pattern (activity) in a data set containing many different types of patterns. Among many approaches proposed to model and detect activities, we have chosen a graph-based technique, Petri Nets, as they do not require training data to model the activity. They use the domain expert's knowledge to build the activity as a combination of feature states and their transitions (actions). Starting from a conceptual model of how hydrothermal plumes respond to daily tides, we have developed a Petri Net based detection algorithm that identifies deviations from the specified response. Initially we assumed that the orientation of the plume would change smoothly and symmetrically in a consistent daily pattern. However, results indicate that the rate of directional changes varies. The present Petri Net detects unusually large and rapid changes in direction or amount of bending; however inspection of Figure 1 suggests that many of the events detected may be artifacts resulting from gaps in the data or from the large temporal spacing. Still, considerable complexity overlies the "normal" tidal response pattern (the data has a dominant frequency of

  5. High abundances of viruses in a deep-sea hydrothermal vent system indicates viral mediated microbial mortality

    Science.gov (United States)

    Ortmann, Alice C.; Suttle, Curtis A.

    2005-08-01

    Little is known about the distribution and abundance of viruses at deep-sea hydrothermal vents. Based on estimates made using epifluorescence microscopy and the dye YoPro-1, much higher viral abundances were observed at active hydrothermal vents than in the surrounding deep sea. This indicates that viral production was occurring and that viruses were a source of microbial mortality. Samples collected from three actively venting sites (Clam Bed, S&M and Salut) within the Endeavour Ridge system off the west coast of North America had viral abundances ranging from 1.45×10 5 to 9.90×10 7 ml -1, while the abundances of prokaryotes ranged from 1.30×10 5 to 4.46×10 6 ml -1. The abundances of viruses and prokaryotes in samples collected along the neutrally buoyant plume associated with the Main Endeavour Field were lower than at actively venting sites, with a mean of 5.3×10 5 prokaryotes ml -1 (s.d. 2.9×10 5, n=64) and 3.50×10 6 viruses ml -1 (s.d. 1.89×10 6, n=64), but were higher than non-plume samples (2.7×10 5 prokaryotes ml -1, s.d. 5.0×10 4, n=15 and 2.94×10 6 viruses ml -1, s.d. 1.08×10 6, n=15). Prokaryotic and viral abundances in non-hydrothermal regions were as much as 10-fold higher than found in previous studies, in which sample fixation likely resulted in underestimates. This suggests that viral infection may be a greater source of prokaryotic mortality throughout the deep sea than previously recognized. Overall, our results indicate that virus-mediated mortality of prokaryotes at these hydrothermal-vent environments is significant and may reduce energy flow to higher trophic levels.

  6. The microbiology of deep-sea hydrothermal vent plumes: ecological and biogeographic linkages to seafloor and water column habitats

    Directory of Open Access Journals (Sweden)

    Gregory J Dick

    2013-05-01

    Full Text Available Hydrothermal plumes are an important yet understudied component of deep-sea vent microbial ecosystems. The significance of plume microbial processes can be appreciated from three perspectives: (1 mediation of plume biogeochemistry, (2 dispersal of seafloor hydrothermal vent microbes between vents sites, (3 as natural laboratories for understanding the ecology, physiology, and function of microbial groups that are distributed throughout the pelagic deep sea. Plume microbiology has been largely neglected in recent years, especially relative to the extensive research conducted on seafloor and subseafloor systems. Rapidly advancing technologies for investigating microbial communities provide new motivation and opportunities to characterize this important microbial habitat. Here we briefly highlight microbial contributions to plume and broader ocean (biogeochemistry and review recent work to illustrate the ecological and biogeographic linkages between plumes, seafloor vent habitats, and other marine habitats such as oxygen minimum zones, cold seeps, and oil spills. 16S rRNA gene surveys and metagenomic/-transcriptomic data from plumes point to dominant microbial populations, genes, and functions that are also operative in oxygen minimum zones (SUP05, ammonia-oxidizing Archaea, and SAR324 Deltaproteobacteria and hydrocarbon-rich environments (methanotrophs. Plume microbial communities are distinct from those on the seafloor or in the subsurface but contain some signatures of these habitats, consistent with the notion that plumes are potential vectors for dispersal of microorganisms between seafloor vent sites. Finally, we put forward three pressing questions for the future of deep-sea hydrothermal plume research and consider interactions between vents and oceans on global scales.

  7. Influence of hydrothermal venting on water column properties in the crater of the Kolumbo submarine volcano, Santorini volcanic field (Greece)

    Science.gov (United States)

    Christopoulou, Maria E.; Mertzimekis, Theo J.; Nomikou, Paraskevi; Papanikolaou, Dimitrios; Carey, Steven; Mandalakis, Manolis

    2016-02-01

    The Kolumbo submarine volcano, located 7 km northeast of the island of Santorini, is part of Santorini's volcanic complex in the south Aegean Sea, Greece. Kolumbo's last eruption was in 1650 AD. However, a unique and active hydrothermal vent field has been revealed in the northern part of its crater floor during an oceanographic survey by remotely operated vehicles (ROVs) in 2006. In the present study, conductivity-temperature-depth (CTD) data collected by ROV Hercules during three oceanographic surveys onboard E/V Nautilus in 2010 and 2011 have served to investigate the distribution of physicochemical properties in the water column, as well as their behavior directly over the hydrothermal field. Additional CTD measurements were carried out in volcanic cone 3 (VC3) along the same volcanic chain but located 3 km northeast of Kolumbo where no hydrothermal activity has been detected to date. CTD profiles exhibit pronounced anomalies directly above the active vents on Kolumbo's crater floor. In contrast, VC3 data revealed no such anomalies, essentially resembling open-sea (background) conditions. Steep increases of temperature (e.g., from 16 to 19 °C) and conductivity near the maximum depth (504 m) inside Kolumbo's cone show marked spatiotemporal correlation. Vertical distributions of CTD signatures suggest a strong connection to Kolumbo's morphology, with four distinct zones identified (open sea, turbid flow, invariable state, hydrothermal vent field). Additionally, overlaying the near-seafloor temperature measurements on an X-Y coordinate grid generates a detailed 2D distribution of the hydrothermal vent field and clarifies the influence of fluid discharges in its formation.

  8. Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge

    Science.gov (United States)

    Bourbonnais, A.; Juniper, S. K.; Butterfield, D. A.; Devol, A. H.; Kuypers, M. M. M.; Lavik, G.; Hallam, S. J.; Wenk, C. B.; Chang, B. X.; Murdock, S. A.; Lehmann, M. F.

    2012-11-01

    Little is known about fixed nitrogen (N) transformation and elimination at diffuse hydrothermal vents where anoxic fluids are mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N loss pathways (denitrification, anammox) and dissimilatory nitrate reduction to ammonium (DNRA) in sulfidic hydrothermal vent fluids discharging from the subsurface at several sites at Axial Volcano and the Endeavour Segment on the Juan de Fuca Ridge. We also measured physico-chemical parameters (i.e., temperature, pH, nutrients, H2S and N2O concentrations) as well as the biodiversity and abundance of chemolithoautotrophic nitrate-reducing, sulfur-oxidizing γ-proteobacteria (SUP05 cluster) using sequence analysis of amplified small subunit ribosomal RNA (16S rRNA) genes in combination with taxon-specific quantitative polymerase chain reaction (qPCR) assays. Denitrification was the dominant N loss pathway in the subsurface biosphere of the Juan de Fuca Ridge, with rates of up to ~1000 nmol N l-1 day-1. In comparison, anammox rates were always hydrothermal vent waters. Taxon-specific qPCR revealed that γ-proteobacteria of the SUP05 cluster sometimes dominated the microbial community (SUP05/total bacteria up to 38%). Significant correlations were found between fixed N loss (i.e., denitrification, anammox) rates and in situ nitrate and dissolved inorganic nitrogen (DIN) deficits in the fluids, indicating that DIN availability may ultimately regulate N loss in the subsurface. Based on our rate measurements, and on published data on hydrothermal fluid fluxes and residence times, we estimated that up to ~10 Tg N yr-1 could globally be removed in the subsurface biosphere of hydrothermal vents systems, thus, representing a small fraction of the total marine N loss (~275 to > 400 Tg N yr-1).

  9. Phylogenetic diversity of nitrogenase (nifH) genes in deep-sea and hydrothermal vent environments of the Juan de Fuca Ridge.

    Science.gov (United States)

    Mehta, Mausmi P; Butterfield, David A; Baross, John A

    2003-02-01

    The subseafloor microbial habitat associated with typical unsedimented mid-ocean-ridge hydrothermal vent ecosystems may be limited by the availability of fixed nitrogen, inferred by the low ammonium and nitrate concentrations measured in diffuse hydrothermal fluid. Dissolved N2 gas, the largest reservoir of nitrogen in the ocean, is abundant in deep-sea and hydrothermal vent fluid. In order to test the hypothesis that biological nitrogen fixation plays an important role in nitrogen cycling in the subseafloor associated with unsedimented hydrothermal vents, degenerate PCR primers were designed to amplify the nitrogenase iron protein gene nifH from hydrothermal vent fluid. A total of 120 nifH sequences were obtained from four samples: a nitrogen-poor diffuse vent named marker 33 on Axial Volcano, sampled twice over a period of 1 year as its temperature decreased; a nitrogen-rich diffuse vent near Puffer on Endeavour Segment; and deep seawater with no detectable hydrothermal plume signal. Subseafloor nifH genes from marker 33 and Puffer are related to anaerobic clostridia and sulfate reducers. Other nifH genes unique to the vent samples include proteobacteria and divergent Archaea. All of the nifH genes from the deep-seawater sample are most closely related to the thermophilic, anaerobic archaeon Methanococcus thermolithotrophicus (77 to 83% amino acid similarity). These results provide the first genetic evidence of potential nitrogen fixers in hydrothermal vent environments and indicate that at least two sources contribute to the diverse assemblage of nifH genes detected in hydrothermal vent fluid: nifH genes from an anaerobic, hot subseafloor and nifH genes from cold, oxygenated deep seawater. PMID:12571018

  10. Characteristics of hydrothermal plumes from two vent fields on the Juan de Fuca Ridge, northeast Pacific Ocean

    Science.gov (United States)

    Baker, Edward T.; Massoth, Gary J.

    1987-09-01

    Deep CTD/transmissometer tows and water bottle sampling were used during 1985 to map the regional distribution of the neutrally-buoyant plumes emanating from each of two major vent fields on the Southern Symmetrical Segment (SSS) and Endeavour Segment (ES) of the Juan de Fuca Ridge. At both vent fields, emissions from point and diffuse hydrothermal sources coalesced into a single 200-m-thick plume elongated in the direction of current flow and with characteristic temperature anomalies of 0.02-0.05°C and light-attenuation anomalies of 0.01-0.08 m -1 (10-80 μg/l above background). Temperature anomalies in the core of each plume were uniform as far downcurrent as the plumes were mapped (10-15 km). Downcurrent light-attenuation trends were non-uniform and differed between plumes, apparently because different vent fluid chemistries at each field cause significant differences in the settling characteristics of the hydrothermal precipitates. Vent fluids from the SSS are metal-dominated and mostly precipitate very fine-grained hydrous Fe-oxides that remain suspended in the plume. Vent fluids from the ES are sulfur-dominated and precipitate a high proportion of coarser-grained Fe-sulfides that rapidly settle from the plume. The integrated flux of each vent field was estimated from measurements of the advective transport of each plume. Heat flux was 1700 ± 1100 MW from the ES and 580 ± 351 MW from the SSS. Particle flux varied from 546 ± 312 g/s to 204 ± 116 g/s at the ES depending on distance from the vent field, and was 92 ± 48 g/s from the SSS.

  11. Spatial differences in East scotia ridge hydrothermal vent food webs: influences of chemistry, microbiology and predation on trophodynamics.

    Directory of Open Access Journals (Sweden)

    William D K Reid

    Full Text Available The hydrothermal vents on the East Scotia Ridge are the first to be explored in the Antarctic and are dominated by large peltospiroid gastropods, stalked barnacles (Vulcanolepas sp. and anomuran crabs (Kiwa sp. but their food webs are unknown. Vent fluid and macroconsumer samples were collected at three vent sites (E2, E9N and E9S at distances of tens of metres to hundreds of kilometres apart with contrasting vent fluid chemistries to describe trophic interactions and identify potential carbon fixation pathways using stable isotopes. δ(13C of dissolved inorganic carbon from vent fluids ranged from -4.6‰ to 0.8‰ at E2 and from -4.4‰ to 1.5‰ at E9. The lowest macroconsumer δ(13C was observed in peltospiroid gastropods (-30.0‰ to -31.1‰ and indicated carbon fixation via the Calvin-Benson-Bassham (CBB cycle by endosymbiotic gamma-Proteobacteria. Highest δ(13C occurred in Kiwa sp. (-19.0‰ to -10.5‰, similar to that of the epibionts sampled from their ventral setae. Kiwa sp. δ(13C differed among sites, which were attributed to spatial differences in the epibiont community and the relative contribution of carbon fixed via the reductive tricarboxylic acid (rTCA and CBB cycles assimilated by Kiwa sp. Site differences in carbon fixation pathways were traced into higher trophic levels e.g. a stichasterid asteroid that predates on Kiwa sp. Sponges and anemones at the periphery of E2 assimilated a proportion of epipelagic photosynthetic primary production but this was not observed at E9N. Differences in the δ(13C and δ(34S values of vent macroconsumers between E2 and E9 sites suggest the relative contributions of photosynthetic and chemoautotrophic carbon fixation (rTCA v CBB entering the hydrothermal vent food webs vary between the sites.

  12. Metaproteomic Analysis of a Chemosynthetic Hydrothermal Vent Community Reveals Insights into Key-Metabolic Processes

    Science.gov (United States)

    Steen, I.; Stokke, R.; Lanzen, A.; Pedersen, R.; Øvreås, L.; Urich, T.

    2010-12-01

    In 2005 researchers at the Centre for Geobiology, University of Bergen, Norway, discovered two active vent fields at the southwestern Mohns Ridge in the Norwegian-Greenland Sea. The fields harbours both low-temperature iron deposits and high-temperature white smoker vents. Distinct microbial mats were abundantly present and located in close vicinity to the hydrothermal vent sites. Characteristics of the mat environment were steep physical and chemical gradients with temperatures ranging from 10°C in the top layer to 90°C at 10 cm bsf and high concentrations of hydrogen sulfide and methane. The work presented here focus on the In situ community activities, and is part of an integrated strategy combining metagenomics, metatranscriptomics and metaproteomics to in-depth characterise these newly discovered hydrothermal vent communities. Extracted proteins were separated via SDS-PAGE. Peptides extracted after In-gel tryptic digest was injected into an Ultimate 3000 nanoLC system connected to a linear quadropole ion trap-orbitrap (LTQ-Orbitrap XL) mass spectrometer equipped with a nanoelectrospray ion source. A custom database of open reading frames (ORFs) from the combined metatranscriptome and metagenome datasets was implemented and searched against using Mascot 2.2; the IRMa tool box [1] was used in peptide validation. Validated ORFs were subjected to a Blastp search against Refseq with an E-value cut-off of 0.001. A total of 1097 proteins with ≥ 2 peptides were identified of which 921 gave a hit against Refseq, containing 519 unique proteins. Key enzymes of the sulfur oxidation pathway (sox) were found, which were taxonomically affiliated to Epsilonproteobacteria. In addition, this group actively expressed hydrogenases and membrane proteins involved in aerobic and anaerobic respiratory chains. Enzymes of dissimilatory sulfate-reduction (APS-reductase, AprAB and DsrA2) were found with closest hit to members of the Deltaproteobacteria. These findings indicate an

  13. Phenotypic variation and fitness in a metapopulation of tubeworms (Ridgeia piscesae Jones at hydrothermal vents.

    Directory of Open Access Journals (Sweden)

    Verena Tunnicliffe

    Full Text Available We examine the nature of variation in a hot vent tubeworm, Ridgeia piscesae, to determine how phenotypes are maintained and how reproductive potential is dictated by habitat. This foundation species at northeast Pacific hydrothermal sites occupies a wide habitat range in a highly heterogeneous environment. Where fluids supply high levels of dissolved sulphide for symbionts, the worm grows rapidly in a "short-fat" phenotype characterized by lush gill plumes; when plumes are healthy, sperm package capture is higher. This form can mature within months and has a high fecundity with continuous gamete output and a lifespan of about three years in unstable conditions. Other phenotypes occupy low fluid flux habitats that are more stable and individuals grow very slowly; however, they have low reproductive readiness that is hampered further by small, predator cropped branchiae, thus reducing fertilization and metabolite uptake. Although only the largest worms were measured, only 17% of low flux worms were reproductively competent compared to 91% of high flux worms. A model of reproductive readiness illustrates that tube diameter is a good predictor of reproductive output and that few low flux worms reached critical reproductive size. We postulate that most of the propagules for the vent fields originate from the larger tubeworms that live in small, unstable habitat patches. The large expanses of worms in more stable low flux habitat sustain a small, but long-term, reproductive output. Phenotypic variation is an adaptation that fosters both morphological and physiological responses to differences in chemical milieu and predator pressure. This foundation species forms a metapopulation with variable growth characteristics in a heterogeneous environment where a strategy of phenotypic variation bestows an advantage over specialization.

  14. Effects of shallow-water hydrothermal venting on biological communities of coastal marine ecosystems of the western Pacific.

    Science.gov (United States)

    Tarasov, V G

    2006-01-01

    This review is based on integrated studies of the composition, structure and function of shallow-water ecosystems in the western Pacific that are influenced by underwater gas-hydrothermal activity. Most of the data were collected from 1985 to 1997 by the Institute of Marine Biology of the Far East Branch of the Russian Academy of Science during expeditions to zones of modern volcanism. Gas-hydrothermal activity of volcanoes has a great influence on the physicochemical characteristics of the water column and plankton, and of bottom sediment and benthic communities. The abundance of nutrients (SiO(3)(2-), PO(4)(3-), NO(3)(-)), gases (CO(2), CH(4), H(2), H(2)S) and other reduced compounds (C(n)H(n), S(0), S(2)O(3)(2-), NH(4)(+)) in zones of shallow-water hydrothermal vents provides conditions for the use of two energy sources for primary production: sunlight (photosynthesis) and the oxidation of reduced compounds (bacterial chemosynthesis). In areas of shallow-water volcanic activity, chemosynthesis occurs not only in the immediate vicinity of venting fluid release but also in the surface layer of the water column, where it occurs together with intense photosynthesis. This surface photosynthesis is found below the layer of chemosynthesis, which is related to the distribution of hydrothermal fluids at the water surface. The contribution of each of these processes to total primary production depends on the physical and chemical conditions created by the vents and on the range and adaptation potential of the organisms. On the seabed in zones of shallow-water venting, microorganisms form mats that consist of bacteria of various physiological groups, microalgae, the products of their metabolism and sedimentary particles. Oxygenic photosynthesis of benthic diatoms, bacterial photosynthesis (anoxygenic photosynthesis) and autotrophic chemosynthesis in algobacterial and bacterial mats generate organic matter additional to that produced in the water column. The high rates of

  15. The complete mitogenome of the Atlantic hydrothermal vent shrimp Rimicaris exoculata Williams & Rona 1986 (Crustacea: Decapoda: Alvinocarididae).

    Science.gov (United States)

    Yu, Yan-Qin; Liu, Xiao-Li; Li, Hua-Wei; Lu, Bo; Fan, Yu-Peng; Yang, Jin-Shu

    2016-09-01

    In this study we completely determined and analyzed the mitochondrial genome of the Mid-Atlantic Ridge hydrothermal-vent shrimp Rimicaris exoculata (Crustacea: Decapoda: Alvinocarididae). The circular molecule is 15,902 bp in size with an AT content of 65.7%, composed of the same 37 mitochondrial genes as in all other known metazoan mitogenomes. Sequence composition of the R. exoculata mitogenome is exceptionally similar to that of its Indian-Ocean congener R. kairei, which suggests the fact that they might diverge at a quite recent age. The genome exhibits an ancestral pancrustacean arrangement of mitochondrial genes that presents only the translocation/inversion of trnL-UUR from the ancestral arthropod pattern. Determination of the R. exoculata mitogenome can help to resolve the consensus Decapoda tree of life. It also provides more genetic information available for phylogenetics as well as population genetics on this extensively studied species from hydrothermal vents. PMID:25665594

  16. Two Novel Hepatocellular Carcinoma Cycle Inhibitory Cyclodepsipeptides from a Hydrothermal Vent Crab-Associated Fungus Aspergillus clavatus C2WU

    Directory of Open Access Journals (Sweden)

    Wei Jiang

    2013-12-01

    Full Text Available Two novel cyclodepsipeptides containing an unusual anthranilic acid dimer and a d-phenyllactic acid residues, clavatustides A (1 and B (2, were identified from cultured mycelia and broth of Aspergillus clavatus C2WU isolated from Xenograpsus testudinatus, which lives at extreme, toxic habitat around the sulphur-rich hydrothermal vents in Taiwan Kueishantao. This is the first example of cyclopeptides containing an anthranilic acid dimer in natural products, and the first report of microbial secondary metabolites from the hydrothermal vent crab. Clavatustides A (1 and B (2 suppressed the proliferation of hepatocellular carcinoma (HCC cell lines (HepG2, SMMC-7721 and Bel-7402 in a dose-dependent manner, and induced an accumulation of HepG2 cells in G1 phase and reduction of cells in S phase.

  17. Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature

    OpenAIRE

    Shillito Bruce; Jollivet Didier; Boutet Isabelle; Moraga Dario; Tanguy Arnaud

    2009-01-01

    Abstract Background Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, d...

  18. Hydrothermal alkaline stability of bentonite barrier by concrete interstitial wastes

    International Nuclear Information System (INIS)

    At present, the main source of High Level radioactive Waste (HLW) is the electrical energy production during all the steps of developing. In almost all the countries with nuclear programs, the option for the final management of HLW is the Deep Geological Repository (DGR) based on the concept of multi barrier. According to this concept, the waste is isolated from biosphere by the interposition of confinement barriers. Two of the engineering barriers in the Spanish design of DGR in granitic rock are compacted bentonite and concrete. The bentonite barrier is the backfilling and sealing material for the repository gallery, because of its mechanical and physico-chemical properties. The main qualities of concrete as a component of a multi barrier system are its low permeability, mechanical resistance and chemical properties. With regard to chemical composition of concrete, the alkaline nature of cement pore water lowers the solubility of many radioactive elements. However, structural transformation in smectite, dissolution or precipitation of minerals and, consequently, changes in the bentonite properties could occurs in the alkaline conditions generated by the cement degradation. The main objective of the present work is to evaluate the effect of concrete in the stability of Spanish reference bentonite (La Serrata of Nijar, Almeria, Spain) in conditions similar to those estimated in a DGR in granitic rock. Because of the main role of bentonite barrier in the global performance of the repository, the present study is essential to guarantee its security. (Author)

  19. Lithosphere-biosphere interaction at a shallow-sea hydrothermal vent site; Hot Lake, Panarea, Italy

    Science.gov (United States)

    Huang, Chia-I.; Amann, Rudolf; Amend, Jan P.; Bach, Wolfgang; Brunner, Benjamin; Meyerdierks, Anke; Price, Roy E.; Schubotz, Florence; Summons, Roger; Wenzhöfer, Frank

    2010-05-01

    Deep-Sea hydrothermal systems are unique habitats for microbial life with primary production based on chemosynthesis and are considered to be windows to the subsurface biosphere. It is often overlooked, however, that their far more accessible shallow-sea counterparts are also valuable targets to study the effects of hydrothermal activity on geology, seawater chemistry and finally, on microbial life. Such an area of shallow marine hydrothermal venting is observed approximately 2.5 km east of Panarea Island (Sicily, Italy). This system is characterized by fluid temperatures of up to 135° C, gas emissions dominated by CO2 and precipitation of elemental sulfur on the seafloor. In an interdisciplinary project to investigate the influence of geofuels on marine microbiota, sediment cores and pore fluids were sampled for geological and geochemical analyses. An attempt was made to link these geochemical data with a characterization of the microbial community. One of the investigated sites (Lago Caldo, Hot Lake) is an oval-shaped (~10 by 6 meters) shallow (~2.5 m deep) depression covered by elemental sulfur. The sediments in this depression are strongly affected by hydrothermal activity: the pH of pore fluids is in a range between 5 and 6; the salinity is approximately two times higher than seawater. In situ temperatures of 36° C and 74° C (10 cm sediment depth) at two different locations within Hot Lake indicate variability in hydrothermal flux. The sediment surface layer is anoxic, and with increasing depth from the sediment-water interface, sulfate concentrations decrease from ~30 mM to less than 10 mM, whereas sulfide concentrations increase from less than 50 μm to ~1000 μm at 25 cm sediment depth, thus suggesting a higher potential for energy gain based on sulfur disequilibrium. As indicated by the variability in the sediment temperatures at 10 cm, fluid fluxes and mixing with seawater is not found to be uniform at Hot Lake. This is reflected in variability of the

  20. Moored observation of abyssal flow and temperature near a hydrothermal vent on the Southwest Indian Ridge

    Science.gov (United States)

    Liao, Guanghong; Zhou, Beifeng; Liang, Chujin; Zhou, Huaiyang; Ding, Tao; Wang, Yuan; Dong, Changming

    2016-01-01

    Four moorings were deployed near "Dragon Flag," an active hydrothermal vent in the valley of the Southwest Indian Ridge. The goal was to examine the variability of currents and temperature, which will guide the trajectory of spreading plumes. The mean current was cross-isobath, and the circulation was characterized by a submesoscale circulation. Observed currents also showed fluctuations with periods of 1-15 days. The inferred phase speed and wavelength for the wave with a period of 4.4 day are 10.4 km d-1 and 45.8km, respectively, which are consistent with the topographic Rossby wave theory. The persistent warming tendency with corresponding variation of salinity based on background θ-S properties may be caused by background circulation and divergence of the water column. The warming or cooling episodes were most likely as signatures of isopycnal surface depression or uplifting induced by the moving of mesoscale eddies. Well-resolved rotary spectra exhibited important nonlinear interactions between inertial and semidiurnal tide in the velocity and temperature records. Amplification of near-inertial currents in the near bottom is also exposed. These discoveries provided new evidence for the nonlinear interaction and trapped near-inertial waves by the ridge, which occurred in the deep ocean of the Southern Hemisphere. Such nonlinear interaction may represent a significant energy loss pathway for the internal waves, and part of the decay of such motion would likely result in increased mixing to maintain the abyssal stratification. Enhanced near-inertial motions can play a major role for the local advection of hydrothermal plumes.

  1. Fe-oxidizing microbes are hydrothermal vent ecosystem engineers at the Loihi Seamount (Invited)

    Science.gov (United States)

    Chan, C. S.; McAllister, S.; Leavitt, A.; Emerson, D.; Moyer, C. L.; Glazer, B. T.

    2013-12-01

    Microaerophilic Fe-oxidizing microorganisms (FeOM) colonize gradients of Fe(II) and oxygen, taking advantage of the available chemical energy. Vast communities of FeOM proliferate at deep sea hydrothermal vents, forming mineralized mats that range from centimeters to meters thick. Because these mats structure the environment for both FeOM and the entire microbial community, the Fe-oxidizers are acting as ecosystem engineers. What organisms are responsible for initiating these mats, and how does the physical structure and community composition develop as the mats mature? By connecting structure, function, and ecology, we can better interpret modern mat structures, as well as ancient fossilized mats. We have been studying Fe microbial mats at Loihi Seamount in Hawaii, a long-term study site that has become a model for Fe oxidation in marine hydrothermal systems. Recent improvements in ROV imaging systems allow us to see a great range of mat textures and colors, which may represent diverse habitats and/or different stages of mat development. With improved imaging and sampling techniques, we have been able to obtain discrete, intact samples of these delicate microbial mats. Previous bulk sampling methods showed that mats consist of a mixture of Fe-mineralized morphologies. Our analyses of intact mats show that mats are initiated by one type of structure-former (either a stalk-former like Mariprofundus ferrooxydans or a Zetaproteobacterial sheath-former). These microbes may be the vanguard organisms that stabilize chemical gradients in this dynamic environment, allowing colonization by other organisms (evidenced by branching tubes, fibrillar nests, and other morphologies). We will show evidence of the composition and development of these mats, and discuss parallels between these marine Fe mats and their freshwater counterparts, supporting the idea that FeOM engineer environments favorable for growth.

  2. Genomic reconstruction of an uncultured hydrothermal vent gammaproteobacterial methanotroph (family Methylothermaceae indicates multiple adaptations to oxygen limitation

    Directory of Open Access Journals (Sweden)

    Connor Tobias Skennerton

    2015-12-01

    Full Text Available Hydrothermal vents are an important contributor to marine biogeochemistry, producing large volumes of reduced fluids, gasses, and metals and housing unique, productive microbial and animal communities fueled by chemosynthesis. Methane is a common constituent of hydrothermal vent fluid and is frequently consumed at vent sites by methanotrophic bacteria that serve to control escape of this greenhouse gas into the atmosphere. Despite their ecological and geochemical importance, little is known about the ecophysiology of uncultured hydrothermal vent-associated methanotrophic bacteria. Using metagenomic binning techniques, we recovered and analyzed a near-complete genome from a novel gammaproteobacterial methanotroph (B42 associated with a white smoker chimney in the Southern Lau basin. B42 was the dominant methanotroph in the community, at ~80x coverage, with only four others detected in the metagenome, all on low coverage contigs (7x - 12x. Phylogenetic placement of B42 showed it is a member of the Methylothermaceae, a family currently represented by only one sequenced genome. Metabolic inferences based on the presence of known pathways in the genome showed that B42 possesses a branched respiratory chain with A- and B-family heme copper oxidases, cytochrome bd oxidase and a partial denitrification pathway. These genes could allow B42 to respire over a wide range of oxygen concentrations within the highly dynamic vent environment. Phylogenies of the denitrification genes revealed they are the result of separate horizontal gene transfer from other proteobacteria and suggest that denitrification is a selective advantage in conditions where extremely low oxygen concentrations require all oxygen to be used for methane activation.

  3. Genomic Reconstruction of an Uncultured Hydrothermal Vent Gammaproteobacterial Methanotroph (Family Methylothermaceae) Indicates Multiple Adaptations to Oxygen Limitation.

    Science.gov (United States)

    Skennerton, Connor T; Ward, Lewis M; Michel, Alice; Metcalfe, Kyle; Valiente, Chanel; Mullin, Sean; Chan, Ken Y; Gradinaru, Viviana; Orphan, Victoria J

    2015-01-01

    Hydrothermal vents are an important contributor to marine biogeochemistry, producing large volumes of reduced fluids, gasses, and metals and housing unique, productive microbial and animal communities fueled by chemosynthesis. Methane is a common constituent of hydrothermal vent fluid and is frequently consumed at vent sites by methanotrophic bacteria that serve to control escape of this greenhouse gas into the atmosphere. Despite their ecological and geochemical importance, little is known about the ecophysiology of uncultured hydrothermal vent-associated methanotrophic bacteria. Using metagenomic binning techniques, we recovered and analyzed a near-complete genome from a novel gammaproteobacterial methanotroph (B42) associated with a white smoker chimney in the Southern Lau basin. B42 was the dominant methanotroph in the community, at ∼80x coverage, with only four others detected in the metagenome, all on low coverage contigs (7x-12x). Phylogenetic placement of B42 showed it is a member of the Methylothermaceae, a family currently represented by only one sequenced genome. Metabolic inferences based on the presence of known pathways in the genome showed that B42 possesses a branched respiratory chain with A- and B-family heme copper oxidases, cytochrome bd oxidase and a partial denitrification pathway. These genes could allow B42 to respire over a wide range of oxygen concentrations within the highly dynamic vent environment. Phylogenies of the denitrification genes revealed they are the result of separate horizontal gene transfer from other Proteobacteria and suggest that denitrification is a selective advantage in conditions where extremely low oxygen concentrations require all oxygen to be used for methane activation. PMID:26779119

  4. Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge

    OpenAIRE

    A. Bourbonnais; S. K. Juniper; Butterfield, D.A.; Devol, A. H.; Kuypers, M. M. M.; Lavik, G.; S. J. Hallam; Wenk, C. B.; B. X. Chang; Murdock, S. A.; Lehmann, M.F.

    2012-01-01

    Little is known about fixed nitrogen (N) transformation and elimination at diffuse hydrothermal vents where anoxic fluids are mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N loss pathways (denitrification, anammox) and dissimilatory nitrate reduction to ammonium (DNRA) in sulfidic hydroth...

  5. Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge

    OpenAIRE

    Bourbonnais, A.; S. K. Juniper; D. A. Butterfield; Devol, A. H.; Kuypers, M. M. M.; G. Lavik; Hallam, S. J.; C. B. Wenk; Chang, B. X.; S. A. Murdock; M. F. Lehmann

    2012-01-01

    Little is known about nitrogen (N) transformations in general, and the elimination of N in particular, at diffuse vents where anoxic hydrothermal fluids have mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N-loss pathways (denitrification, anammox) and dissimilativ...

  6. Dietary carbon sources of mussels and tubeworms from Galapagos hydrothermal vents determined from tissue 14C activity

    International Nuclear Information System (INIS)

    The results of an investigation of the dietary carbon sources of mussels and tubeworms from Galapagos hydrothermal vents, using data from 14C and 13C/12C ratio measurements in tissues, are reported. It is shown that: (1) filter-feeding organisms in the vent system are directly or indirectly incorporating 'dead' carbon of magmatic origin into their tissues; (2) approximately 25% or less of the dietary carbon available to the mussels is from sedimenting particulate organic carbon fixed photosynthetically at the surface; and (3) mussel tissue is incorporating relatively more 'dead' dissolved inorganic carbon than is mussel shell carbonate in specimens collected at the same location near the vent. (U.K.)

  7. Absolute Magnetization Distribution on Back-arc Spreading Axis Hosting Hydrothermal Vents; Insight from Shinkai 6500 Magnetic Survey

    Science.gov (United States)

    Fujii, M.; Okino, K.; Honsho, C.; Mochizuki, N.; Szitkar, F.; Dyment, J.

    2013-12-01

    Near-bottom magnetic profiling using submersible, deep-tow, Remotely Operated Vehicle (ROV) and Autonomous Underwater Vehicle (AUV) make possible to conduct high-resolution surveys and depict detailed magnetic features reflecting, for instance, the presence of fresh lavas or hydrothermal alteration, or geomagnetic paleo-intensity variations. We conducted near-bottom three component magnetic measurements onboard submersible Shinkai 6500 in the Southern Mariana Trough, where five active hydrothermal vent fields (Snail, Yamanaka, Archean, Pica, and Urashima sites) have been found in both on- and off-axis areas of the active back-arc spreading center, to detect signals from hydrothermally altered rock and to distinguish old and new submarine lava flows. Fourteen dives were carried out at an altitude of 1-40 m during the R/V Yokosuka YK10-10 and YK10-11 cruises in 2010. We carefully corrected the effect of the induced and permanent magnetizations of the submersible by applying the correction method for the shipboard three-component magnetometer measurement modified for deep-sea measurement, and subtracted the IGRF values from the corrected data to obtain geomagnetic vector anomalies along the dive tracks. We then calculated the synthetic magnetic vector field produced by seafloor, assumed to be uniformly magnetized, using three dimensional forward modeling. Finally, values of the absolute magnetizations were estimated by using a linear transfer function in the Fourier domain from the observed and synthetic magnetic anomalies. The distribution of estimated absolute magnetization generally shows low values around the five hydrothermal vent sites. This result is consistent with the equivalent magnetization distribution obtained from previous AUV survey data. The areas of low magnetization are also consistent with hydrothermal deposits identified in video records. These results suggest that low magnetic signals are due to hydrothermal alteration zones where host rocks are

  8. Exploring the limit of metazoan thermal tolerance via comparative proteomics: thermally induced changes in protein abundance by two hydrothermal vent polychaetes

    OpenAIRE

    Dilly, G.F.; Young, C R; Lane, W S; Pangilinan, J.; Peter R. Girguis

    2012-01-01

    Temperatures around hydrothermal vents are highly variable, ranging from near freezing up to 300°C. Nevertheless, animals thrive around vents, some of which live near the known limits of animal thermotolerance. Paralvinella sulfincola, an extremely thermotolerant vent polychaete, and Paralvinella palmiformis, a cooler-adapted congener, are found along the Juan de Fuca Ridge in the northwestern Pacific. We conducted shipboard high-pressure thermotolerance experiments on both species to charact...

  9. Spatial Variation in the Population Structure and Reproductive Biology of Rimicaris hybisae (Caridea: Alvinocarididae) at Hydrothermal Vents on the Mid-Cayman Spreading Centre

    OpenAIRE

    Verity Nye; Copley, Jonathan T.; Tyler, Paul A.

    2013-01-01

    The dynamics and microdistribution of faunal assemblages at hydrothermal vents often reflect the fine-scale spatial and temporal heterogeneity of the vent environment. This study examined the reproductive development and population structure of the caridean shrimp Rimicaris hybisae at the Beebe and Von Damm Vent Fields (Mid-Cayman Spreading Centre, Caribbean) using spatially discrete samples collected in January 2012. Rimicaris hybisae is gonochoric and exhibits iteroparous reproduction. Oocy...

  10. Iron oxide deposits associated with the ectosymbiotic bacteria in the hydrothermal vent shrimp Rimicaris exoculata

    Directory of Open Access Journals (Sweden)

    P. Compère

    2008-09-01

    Full Text Available The Rimicaris exoculata shrimp is considered as a primary consumer that dominates the fauna of most Mid-Atlantic Ridge (MAR hydrothermal ecosystems. These shrimps harbour in their gill chambers an important ectosymbiotic community of chemoautotrophic bacteria associated with iron oxide deposits. The structure and elemental composition of the mineral concretions associated with these bacteria have been investigated by using LM, ESEM, TEM STEM and EDX microanalyses. The nature of the iron oxides in shrimps obtained from the Rainbow vent field has also been determined by Mössbauer spectroscopy. This multidisciplinary approach has revealed that the three layers of mineral crust in the Rimicaris exoculata shrimps consist of large concretions formed by aggregated nanoparticles of two-line ferrihydrite and include other minor elements as Si, Ca, Mg, S and P, probably present as silicates cations, sulphates or phosphates respectively that may contribute to stabilise the ferrihydrite form of iron oxides. TEM-observations on the bacteria have revealed their close interactions with these minerals. Abiotic and biotic precipitation could occur within the gill chamber of Rimicaris exoculata, suggesting the biologically-mediated formation of the iron oxide deposits. The difference of the bacterial density in the three-mineral crust layers could be correlated to the importance of the iron oxide concretions and suggest that the first mineral particles precipitates on the lower layer which could be considered as the most likely location of iron-oxidizing bacteria.

  11. The Biological Deep Sea Hydrothermal Vent as a Model to Study Carbon Dioxide Capturing Enzymes

    Directory of Open Access Journals (Sweden)

    Premila D. Thongbam

    2011-04-01

    Full Text Available Deep sea hydrothermal vents are located along the mid-ocean ridge system, near volcanically active areas, where tectonic plates are moving away from each other. Sea water penetrates the fissures of the volcanic bed and is heated by magma. This heated sea water rises to the surface dissolving large amounts of minerals which provide a source of energy and nutrients to chemoautotrophic organisms. Although this environment is characterized by extreme conditions (high temperature, high pressure, chemical toxicity, acidic pH and absence of photosynthesis a diversity of microorganisms and many animal species are specially adapted to this hostile environment. These organisms have developed a very efficient metabolism for the assimilation of inorganic CO2 from the external environment. In order to develop technology for the capture of carbon dioxide to reduce greenhouse gases in the atmosphere, enzymes involved in CO2 fixation and assimilation might be very useful. This review describes some current research concerning CO2 fixation and assimilation in the deep sea environment and possible biotechnological application of enzymes for carbon dioxide capture.

  12. Rare-earth elements and uranium in high-temperature solutions from East Pacific Rise hydrothermal vent field (130N)

    International Nuclear Information System (INIS)

    The mobility of rare-earth elements (REE) and U during hydrothermal alteration of the basalts at spreading centres has long been a matter of concern because of its bearing on the evolution and recycling of the oceanic crust. Previous approaches to this problem have been indirect, through studies on altered dredged basalts or ophiolites. Sampling of hydrothermal vent waters from the East Pacific Rise (EPR) at 130 N is reported. It provides the first direct evidence of REE-enriched solutions which, however, leave the budget of these elements in the crust and the ocean rather unmodified. In constrast, uranium, like magnesium, is quantitatively taken up from the seawater during the hydrothermal process. (author)

  13. Microbial anaerobic methane cycling in the subseafloor at the Von Damm hydrothermal vent field, Mid-Cayman Rise

    Science.gov (United States)

    Huber, J. A.; Reveillaud, J. C.; Stepanauskas, R.; McDermott, J. M.; Sylva, S. P.; Seewald, J.

    2013-12-01

    The Mid-Cayman Rise (MCR) is Earth's deepest and slowest spreading mid-ocean ridge located in the western Caribbean. With an axial rift valley floor at a depth of ~4200-6500 m, it represents one of the deepest sections of ridge crest worldwide. In 2009, the world's deepest hydrothermal vents (Piccard at 4960 m) and an ultramafic-influenced system only 20 km away on top of an oceanic core complex (Von Damm at 2350 m) were discovered along the MCR. Each site is hosted in a distinct geologic setting with different thermal and chemical regimes. The Von Damm site is a particularly interesting location to examine chemolithoautotrophic subseafloor microbial communities due to the abundant hydrogen, methane, and organic compounds in the venting fluids. Here, we used a combination of stable isotope tracing, next-generation sequencing, and single cell techniques to determine the identity, activity, and genomic repertoire of subseafloor anaerobic archaea involved in methane cycling in hydrothermal fluids venting at the Von Damm site. Molecular sequencing of phylogenetic marker genes revealed the presence of diverse archaea that both generate and consume methane across a geochemical and thermal spectrum of vents. Stable isotope tracing experiments were used to detect biological utilization of formate and dissolved inorganic carbon, and methane generation at 70 °C under anaerobic conditions. Results indicate that methanogenesis with formate as a substrate is occurring at 70 °C at two Von Damm sites, Ginger Castle and the Main Orifice. The results are consistent with thermodynamic predictions for carbon speciation at the temperatures encountered at the ultramafic-hosted Von Damm, where formate is predicted to be thermodynamically stable, and may thus serve as a an important source of carbon. Diverse thermophilic methanogenic archaea belonging to the genera Methanothermococcus were detected at all vent sites with both 16S rRNA tag sequencing and single cell sorting. Other

  14. Adaptations to Hydrothermal Vent Life in Kiwa tyleri, a New Species of Yeti Crab from the East Scotia Ridge, Antarctica.

    Directory of Open Access Journals (Sweden)

    Sven Thatje

    Full Text Available Hydrothermal vents in the Southern Ocean are the physiologically most isolated chemosynthetic environments known. Here, we describe Kiwa tyleri sp. nov., the first species of yeti crab known from the Southern Ocean. Kiwa tyleri belongs to the family Kiwaidae and is the visually dominant macrofauna of two known vent sites situated on the northern and southern segments of the East Scotia Ridge (ESR. The species is known to depend on primary productivity by chemosynthetic bacteria and resides at the warm-eurythermal vent environment for most of its life; its short-range distribution away from vents (few metres is physiologically constrained by the stable, cold waters of the surrounding Southern Ocean. Kiwa tylerihas been shown to present differential life history adaptations in response to this contrasting thermal environment. Morphological adaptations specific to life in warm-eurythermal waters, as found on - or in close proximity of - vent chimneys, are discussed in comparison with adaptations seen in the other two known members of the family (K. hirsuta, K. puravida, which show a preference for low temperature chemosynthetic environments.

  15. Previously unsuspected dietary habits of hydrothermal vent fauna: the bactivorous shrimp Rimicaris hybisae can be carnivorous or even cannibalistic

    Science.gov (United States)

    Versteegh, Emma; Van Dover, Cindy; Coleman, Max

    2014-05-01

    Most hydrothermal vents support productive communities, with chemosynthetic bacteria at the base of the food web. They form a potentially important link in global geochemical cycles. However, few data yet exist on their significance in ocean biogeochemistry and related ecological processes. We present results on the structure of part of the food web around hydrothermal vents of the Mid-Cayman Rise (MCR), revealing previously unknown life-history traits of the alvinocarid shrimp species Rimicaris hybisae. We also demonstrate that stable carbon isotope ratios (δ13C values) are an excellent tracer of trophic positions in these ecosystems, in spite of recent findings arguing otherwise. Two hydrothermal vent fields have been described at the ultra-slow spreading ridge of the MCR. These include the world's deepest hydrothermal vents (Piccard field ~4985 m), which support a food web, which includes bactivorous shrimp and carnivorous anemones. The nearby Von Damm vent field (~2300 m) supports a more complex food web, with more primary producers, and probably some influx of photosynthetically produced carbon. Rimicaris hybisae is abundant at both known MCR vent fields and shows a high degree of spatial variability in population structure and reproductive features. In previous work it has been considered bactivorous. Large variations in tissue δ13C values remained largely unexplained, and it has been argued that δ13C values are not a good food web tracer in hydrothermal vent ecosystems. We observed that shrimp tended to be either in dense aggregations on active chimneys or more sparsely distributed, peripheral shrimp in ambient or near-ambient temperatures. With the hypothesis that varying δ13C values show real differences in food sources between individuals and that shrimp in different locales might have different diets, we collected shrimp from both environments at the Von Damm site during E/V Nautilus (NA034, August 2013) and examined their gut contents. Stomach

  16. Hydrothermal fluids vented at shallow depths at the Aeolian islands: relationships with volcanic and geothermal systems.

    Science.gov (United States)

    Italiano, Francesco; Caracausi, Antonio; Longo, Manfredi; Maugeri, Roberto; Paonita, Antonio

    2010-05-01

    Scuba diving investigations carried out over the last two decades at the Aeolian islands revealed the existence of submarine magmatic and late-magmatic hydrothermalism at all the islands, despite the absence of on-shore activity at some of the islands. The results gained by diving activities provided useful information to evaluate the volcanic and geothermal activity and to manage the volcanic crisis occurred on November 2002 off the island of Panarea. Scuba diving investigations carried out from middle 80's, had shown that despite the absence of on shore volcanic manifestations, submarine hydrothermal activity is recognizable at shallow depth around all the Aeolian islands related either to volcanic and geothermal activity. The sampled gases are CO2-dominated with low amounts of oxygen and reactive gases (H2, CO, CH4 and H2S) with concentrations ranging from a few ppm to some mole percent. Sometimes significant N2 amount are detectable together with high helium contents. Samples having low CO2 content, besides relevant N2 and He amounts, are the consequence of CO2 dissolution in sea-water due to gas-water interactions (GWI) occurred before the sample collection. The high CO2 solubility (878 ml/l, T=20°C, P=1bar) may, in fact, decrease the CO2 content in the venting gases thus increasing the concentrations of the less soluble species (e.g. He 8 ml/l, CO 23 ml/l and CH4 33.8 ml/l) in the gas mixture. Such a process might occur at any level, however, because of the slow water circulation in deep sediments, CO2 is able to saturate the circulating sea-water. The isotopic composition of carbon displays a small range of values while helium isotopes are in the range of 4.1extinct Volcanoes, their chemical composition is similar. Contrastingly the isotope composition of helium shows a large heterogeneity with the highest isotopic ratios surprisingly measured at the extinct volcanic islands in the western sector, and much lower values detected in venting gases from active

  17. Recent Investigation of In-Situ pH in Hydrothermal Vent Fluids at Main Endeavour Field (MEF) and ASHES Vent Field (ASHES): Implications for Dynamic Changes in Subseafloor Hydrothermal System

    Science.gov (United States)

    Ding, K.; Seyfried, W. E., Jr.; Tan, C.; Schaen, A. T.; Luhmann, A. J.

    2014-12-01

    In-situ pH is among the key factors affecting chemical reactions involved with fluid-rock interaction and metal transport in hydrothermal systems. A small variation in pH will often result in a large difference in dissolved metal concentrations. For instance, at 400oC, a decrease of ~0.15 pH unit will cause dissolved Fe concentration to double in fluid coexisting with a Fe-bearing mineral assemblage. This parameter also offers us an opportunity to better understand processes controlling the temporal evolution of hydrothermal vent fluid chemistry at mid-ocean ridges. During our recent cruise AT 26-17 with newly upgraded DSV2 Alvin, in-situ measurements of pH were carried out along with gas-tight sampling of vent fluids. Our efforts were focused at MEF and ASHES on the Juan de Fuca Ridge. These hydrothermal systems have been shown to be particularly responsive to subseafloor seismic and magmatic events. The measured fluid temperature was approximately 333˚C and 300˚C at Dante vent orifice of MEF and Inferno vent orifice of ASHES, respectively. The corresponding measured in-situ pH values for both vents are: 4.94 and 4.88, respectively. Dissolved gases and other species were also measured from gas-tight fluid samples providing a means of comparison with the in-situ data. As we have known the earthquake and magmatic activity often places the system at higher temperature and more reducing conditions in connection with a new evolutionary cycle. Comparing these relatively low in-situ pH values with those measured in the past, especially with the ones obtained at MEF in 1999 after an intense swarm of earthquakes, we see the system trending towards more acidic conditions along with decreasing temperature and dissolved H2 and H2S. Taking an example from Dante vent site, in-situ pH value of 5.15 was recorded with a measured temperature of 363oC two month after the event in 1999, which gives 0.2 pH unit greater than the more recent data. Measured dissolved H2 and H2S

  18. A hybrid zone between Bathymodiolus mussel lineages from eastern Pacific hydrothermal vents

    Directory of Open Access Journals (Sweden)

    Johnson Shannon B

    2013-01-01

    Full Text Available Abstract Background The inhabitants of deep-sea hydrothermal vents occupy ephemeral island-like habitats distributed sporadically along tectonic spreading-centers, back-arc basins, and volcanically active seamounts. The majority of vent taxa undergo a pelagic larval phase, and thus varying degrees of geographical subdivision, ranging from no impedance of dispersal to complete isolation, often exist among taxa that span common geomorphological boundaries. Two lineages of Bathymodiolus mussels segregate on either side of the Easter Microplate, a boundary that separates the East Pacific Rise from spreading centers connected to the Pacific-Antarctic Ridge. Results A recent sample from the northwest flank of the Easter Microplate contained an admixture of northern and southern mitochondrial haplotypes and corresponding alleles at five nuclear gene loci. Genotypic frequencies in this sample did not fit random mating expectation. Significant heterozygote deficiencies at nuclear loci and gametic disequilibria between loci suggested that this transitional region might be a ‘Tension Zone’ maintained by immigration of parental types and possibly hybrid unfitness. An analysis of recombination history in the nuclear genes suggests a prolonged history of parapatric contact between the two mussel lineages. We hereby elevate the southern lineage to species status as Bathymodiolus antarcticus n. sp. and restrict the use of Bathymodiolus thermophilus to the northern lineage. Conclusions Because B. thermophilus s.s. exhibits no evidence for subdivision or isolation-by-distance across its 4000 km range along the EPR axis and Galápagos Rift, partial isolation of B. antarcticus n. sp. requires explanation. The time needed to produce the observed degree of mitochondrial differentiation is consistent with the age of the Easter Microplate (2.5 to 5.3 million years. The complex geomorphology of the Easter Microplate region forces strong cross-axis currents that

  19. Spatial organization of food webs along habitat gradients at deep-sea hydrothermal vents on Axial Volcano, Northeast Pacific

    Science.gov (United States)

    Levesque, Christian; Kim Juniper, S.; Limén, Helene

    2006-04-01

    Deep-sea hydrothermal vents are characterized by steep spatial gradients and high temporal variability in habitat conditions. This leads to the organization of species distribution along spatial habitat gradients, which may constrain food resource utilization and food web structure. We conducted a stable-isotope-based study to test the hypothesis that food resource utilization is constrained by spatial habitat variability at diffuse hydrothermal vents on Axial Volcano, Northeast Pacific. Our study included the ten most biomass-prominent species and considered the temporal change in food web structure at recently created vent sites during three consecutive years. We related species average stable isotopic composition to their position between the center and the periphery of vent sites, using previously published data. Species spread widely along the δ13C axis, and showed a small variability in δ15N. This indicates that most species partition food resources between isotopically different carbon sources, and that they are not organized along predator-prey trophic chains. Particulate organic matter (POM) stable isotopic composition from a concomitant study corresponds to the signature of the expected diet for most organisms. Species average δ13C was significantly correlated to their relative position between the center and the periphery of vent sites. We relate this spatial variability in species isotopic composition to variability in the isotopic signature of both dissolved inorganic carbon (DIC) and POM. This spatial isotopic signal of consumers reveals the spatial structuring of food (POM) production and its consumption by the fauna. Accrual of species during the development of diffuse sites increased the inter-specific spread in δ13C, but did not increase the range in δ15N. Our results show that the spatial organization of species distribution results in a fragmented food web where species partition POM food resources according to their position in space

  20. Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge

    Science.gov (United States)

    Bourbonnais, A.; Juniper, S. K.; Butterfield, D. A.; Devol, A. H.; Kuypers, M. M. M.; Lavik, G.; Hallam, S. J.; Wenk, C. B.; Chang, B. X.; Murdock, S. A.; Lehmann, M. F.

    2012-11-01

    Little is known about fixed nitrogen (N) transformation and elimination at diffuse hydrothermal vents where anoxic fluids are mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N loss pathways (denitrification, anammox) and dissimilatory nitrate reduction to ammonium (DNRA) in sulfidic hydrothermal vent fluids discharging from the subsurface at several sites at Axial Volcano and the Endeavour Segment on the Juan de Fuca Ridge. We also measured physico-chemical parameters (i.e., temperature, pH, nutrients, H2S and N2O concentrations) as well as the biodiversity and abundance of chemolithoautotrophic nitrate-reducing, sulfur-oxidizing γ-proteobacteria (SUP05 cluster) using sequence analysis of amplified small subunit ribosomal RNA (16S rRNA) genes in combination with taxon-specific quantitative polymerase chain reaction (qPCR) assays. Denitrification was the dominant N loss pathway in the subsurface biosphere of the Juan de Fuca Ridge, with rates of up to ~1000 nmol N l-1 day-1. In comparison, anammox rates were always bacteria up to 38%). Significant correlations were found between fixed N loss (i.e., denitrification, anammox) rates and in situ nitrate and dissolved inorganic nitrogen (DIN) deficits in the fluids, indicating that DIN availability may ultimately regulate N loss in the subsurface. Based on our rate measurements, and on published data on hydrothermal fluid fluxes and residence times, we estimated that up to ~10 Tg N yr-1 could globally be removed in the subsurface biosphere of hydrothermal vents systems, thus, representing a small fraction of the total marine N loss (~275 to > 400 Tg N yr-1).

  1. Discovery of hydrothermal venting community at the base of Cambrian barite in Guizhou Province, Western China:Implication for the Cambrian biological explosion

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    The barite deposit in the Niutitang Formation of Lower Cambrian, in Tianzhu County, Guizhou Province, western China, is a superlarge barite deposit with about 2 × 109 tons of reserves: Mineral, petrological and geochemical studies reveal that this barite deposit belongs to a hydrothermal sedimentary deposit. Microscopic observations indicate that a lot of algae, sponge spicules and tube-type fossils are well-preserved in this barite section, and moreover, those fossils share most characteristics of the deep-sea hydrothermal venting community in the modern Pacific. We suggest that the hydrothermal venting community was thriving in hydrothermal vent in early Cambrian, and it is of great significance for elucidating the geological background of the "Cambrian explosion".

  2. A Mossbauer investigation of iron-rich terrestrial hydrothermal vent systems: lessons for Mars exploration

    Science.gov (United States)

    Wade, M. L.; Agresti, D. G.; Wdowiak, T. J.; Armendarez, L. P.; Farmer, J. D.

    1999-01-01

    Hydrothermal spring systems may well have been present on early Mars and could have served as a habitat for primitive life. The integrated instrument suite of the Athena Rover has, as a component on the robotic arm, a Mossbauer spectrometer. In the context of future Mars exploration we present results of Mossbauer analysis of a suite of samples from an iron-rich thermal spring in the Chocolate Pots area of Yellowstone National Park (YNP) and from Obsidian Pool (YNP) and Manitou Springs, Colorado. We have found that Mossbauer spectroscopy can discriminate among the iron-bearing minerals in our samples. Those near the vent and on the surface are identified as ferrihydrite, an amorphous ferric mineraloid. Subsurface samples, collected from cores, which are likely to have undergone inorganic and/or biologically mediated alteration (diagenesis), exhibit spectral signatures that include nontronite (a smectite clay), hematite (alpha-Fe2O3), small-particle/nanophase goethite (alpha-FeOOH), and siderite (FeCO3). We find for iron minerals that Mossbauer spectroscopy is at least as efficient in identification as X-ray diffraction. This observation is important from an exploration standpoint. As a planetary surface instrument, Mossbauer spectroscopy can yield high-quality spectral data without sample preparation (backscatter mode). We have also used field emission scanning electron microscopy (FESEM), in conjunction with energy-dispersive X ray (EDX) fluorescence spectroscopy, to characterize the microbiological component of surface sinters and the relation between the microbiological and the mineralogical framework. Evidence is presented that the minerals found in these deposits can have multi-billion-year residence times and thus may have survived their possible production in a putative early Martian hot spring up to the present day. Examples include the nanophase property and the Mossbauer signature for siderite, which has been identified in a 2.09-billion-year old hematite

  3. Pontibacter amylolyticus sp. nov., isolated from a deep-sea sediment hydrothermal vent field.

    Science.gov (United States)

    Wu, Yue-Hong; Zhou, Peng; Jian, Shu-Ling; Liu, Zhen-Sheng; Wang, Chun-Sheng; Oren, Aharon; Xu, Xue-Wei

    2016-04-01

    A Gram-stain-negative, short rod-shaped bacterium, designated 9-2T, was isolated from a sediment sample collected from a hydrothermal vent field on the south-west Indian Ridge. It formed red colonies, produced carotenoid-like pigments and did not produce bacteriochlorophyll a. Strain 9-2T was positive for hydrolysis of DNA, gelatin and starch, but negative for hydrolysis of aesculin and Tween 60. The sole respiratory quinone was menaquinone-7 (MK-7). The main polar lipids consisted of phosphatidylethanolamine, one unidentified phospholipid and two unidentified polar lipids. The principal fatty acids (>5%) were summed feature 4 (iso-C17:1 I and/or anteiso-C17:1 B), iso-C15:0 and iso-C17:0 3-OH. The genomic DNA G+C content was 49.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 9-2T should be assigned to the genus Pontibacter. Levels of 16S rRNA gene sequence similarity between the new isolate and the type strains of Pontibacter species with validly published names were in the range 94.0-96.5%. On the basis of phenotypic and genotypic data, strain 9-2T represents a novel species of the genus Pontibacter, for which the name Pontibacter amylolyticus sp. nov. is proposed. The type strain is 9-2T (=CGMCC 1.12749T=JCM 19653T=MCCC 1K00278T). PMID:26827710

  4. Free energy generation and transfers from Archaean hydrothermal vents to the first metabolism

    Science.gov (United States)

    Simoncini, E.; Kleidon, A.

    2010-12-01

    Dissipative structures are far from equilibrium systems which self - organize, maintaining a certain internal material order and require free energy in order to be conserved. From a geological point of view, thermal gradients were the most abundant sources of free energy on the early Earth. Here we demonstrate how chemical free energy can be produced by a geological process, serpentinization, associated to the electrochemical potential generation in off - axis hydrothermal vents. The basis for chemical free energy generation is the thermal gradient between the crust and the Archean ocean, which is enhanced by the release of latent heat during serpentinization. Power can be extracted from this thermal gradient to generate motion. The convective motion of heated, chemically reduced fluid produces a redox front when in contact with the acidic Archaean ocean, generating electrical energy to be used in chemical reactions. Further, in the presence of porous inorganic, heterogeneous matrices acting as catalysts, self - sustained reaction chains raised. The free energy thus available could be used to allow the possibility for the establishment of first organic auto - catalytic chains. Molecular evolutionary steps from acetyl CoA to RNA-cleavage gave then rise to the first proto-metabolic processes. We use simple models to calculate the maximum rates of power transfer from the thermal gradient to electric energy to estimate the maximum possible rate of chemical free energy generation by this process. The model also takes into account the heterogeneity of the mineral matrix and its capability to catalyze reactions and to adsorb molecules selectively. In conclusion, non equilibrium thermodynamics in combination with maximum power assumptions help us to determine the fundamental limits of how much chemical free energy can be generated from a geothermal heat flux, providing conditions for the emergence of metabolism.

  5. Expression patterns of mRNAs for methanotrophy and thiotrophy in symbionts of the hydrothermal vent mussel Bathymodiolus puteoserpentis.

    Science.gov (United States)

    Wendeberg, Annelie; Zielinski, Frank U; Borowski, Christian; Dubilier, Nicole

    2012-01-01

    The hydrothermal vent mussel Bathymodiolus puteoserpentis (Mytilidae) from the Mid-Atlantic Ridge hosts symbiotic sulfur- and methane-oxidizing bacteria in its gills. In this study, we investigated the activity and distribution of these two symbionts in juvenile mussels from the Logatchev hydrothermal vent field (14°45'N Mid-Atlantic Ridge). Expression patterns of two key genes for chemosynthesis were examined: pmoA (encoding subunit A of the particulate methane monooxygenase) as an indicator for methanotrophy, and aprA (encoding the subunit A of the dissimilatory adenosine-5'-phosphosulfate reductase) as an indicator for thiotrophy. Using simultaneous fluorescence in situ hybridization (FISH) of rRNA and mRNA we observed highest mRNA FISH signals toward the ciliated epithelium where seawater enters the gills. The levels of mRNA expression differed between individual specimens collected in a single grab from the same sampling site, whereas no obvious differences in symbiont abundance or distribution were observed. We propose that the symbionts respond to the steep temporal and spatial gradients in methane, reduced sulfur compounds and oxygen by modifying gene transcription, whereas changes in symbiont abundance and distribution take much longer than regulation of mRNA expression and may only occur in response to long-term changes in vent fluid geochemistry. PMID:21734728

  6. Cinnabar, arsenian pyrite and thallium-enrichment in active shallow submarine hydrothermal vents at Paleochori Bay, Milos Island, Greece

    Science.gov (United States)

    Kati, Marianna; Voudouris, Panagiotis; Valsami-Jones, Eugenia; Magganas, Andreas; Baltatzis, Emmanouil; Kanellopoulos, Christos; Mavrogonatos, Constantinos

    2015-04-01

    We herein report the discovery of active cinnabar-depositing hydrothermal vents in a submarine setting at Paleochori Bay, within the offshore southeastern extension of the Milos Island Geothermal Field, South Aegean Active Volcanic Arc. Active, low temperature (up to 115 °C) hydrothermal venting through volcaniclastic material has led to a varied assemblage of sulfide and alteration mineral phases in an area of approximately 1 km2. Our samples recovered from Paleochori Bay are hydrothermal edifices composed of volcaniclastic detrital material cemented by pyrite, or pure sulfide (mainly massive pyrite) mounts. Besides pyrite and minor marcasite, the hydrothermal minerals include cinnabar, amorphous silica, hydrous ferric oxides, carbonates (aragonite and calcite), alunite-jarosite solid solution and Sr-rich barite. Among others, growth textures, sieve-textured pyrite associated with barite, alunite-jarosite solid solution and hydrous ferric oxides rims colloform-banded pyrite layers. Overgrowths of arsenian pyrite layers (up to 3.2 wt. % As and/or up to 1.1 wt. % Mn) onto As-free pyrite indicate fluctuation in As content of the hydrothermal fluid. Mercury, in the form of cinnabar, occurs in up to 5 μm grains within arsenian pyrite layers, usually forming distinct cinnabar-enriched micro-layers. Hydrothermal Sr-rich barite (barite-celestine solid solution), pseudocubic alunite-jarosite solid solution and Mn- and Sr-enriched carbonates occur in various amounts and closely associated with pyrite and/or hydrous ferric oxides. Thallium-bearing sulfides and/or sulfosalts were not detected during our study; however, hydrous ferric oxides show thallium content of up to 0.5 wt. % Tl. The following scenarios may have played a role in pyrite precipitation at Paleochori: (a) H2S originally dissolved in the deep fluid but separated upon boiling could have reacted with oxygenated seawater under production of sulphuric acid, thus causing leaching and dissolution of primary iron

  7. Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge

    Directory of Open Access Journals (Sweden)

    A. Bourbonnais

    2012-11-01

    Full Text Available Little is known about fixed nitrogen (N transformation and elimination at diffuse hydrothermal vents where anoxic fluids are mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N loss pathways (denitrification, anammox and dissimilatory nitrate reduction to ammonium (DNRA in sulfidic hydrothermal vent fluids discharging from the subsurface at several sites at Axial Volcano and the Endeavour Segment on the Juan de Fuca Ridge. We also measured physico-chemical parameters (i.e., temperature, pH, nutrients, H2S and N2O concentrations as well as the biodiversity and abundance of chemolithoautotrophic nitrate-reducing, sulfur-oxidizing γ-proteobacteria (SUP05 cluster using sequence analysis of amplified small subunit ribosomal RNA (16S rRNA genes in combination with taxon-specific quantitative polymerase chain reaction (qPCR assays. Denitrification was the dominant N loss pathway in the subsurface biosphere of the Juan de Fuca Ridge, with rates of up to ~1000 nmol N l−1 day−1. In comparison, anammox rates were always < 5 nmol N l−1 day−1 and below the detection limit at most of the sites. DNRA rates were up to ~150 nmol N l−1 day−1. These results suggest that bacterial denitrification out-competes anammox in sulfidic hydrothermal vent waters. Taxon-specific qPCR revealed that γ-proteobacteria of the SUP05 cluster sometimes dominated the microbial community (SUP05/total bacteria up to 38%. Significant correlations were found between fixed N loss (i.e., denitrification, anammox rates and in situ nitrate and dissolved inorganic nitrogen (DIN deficits in the fluids, indicating that DIN availability may ultimately regulate N loss in the subsurface

  8. Activity and abundance of denitrifying bacteria in the subsurface biosphere of diffuse hydrothermal vents of the Juan de Fuca Ridge

    Directory of Open Access Journals (Sweden)

    A. Bourbonnais

    2012-04-01

    Full Text Available Little is known about nitrogen (N transformations in general, and the elimination of N in particular, at diffuse vents where anoxic hydrothermal fluids have mixed with oxygenated crustal seawater prior to discharge. Oceanic N sinks that remove bio-available N ultimately affect chemosynthetic primary productivity in these ecosystems. Using 15N paired isotope techniques, we determined potential rates of fixed N-loss pathways (denitrification, anammox and dissimilative nitrate reduction to ammonium (DNRA in sulfidic hydrothermal vent fluids discharging from the subsurface at several sites at Axial Volcano and the Endeavour Segment on the Juan de Fuca Ridge. We also measured physico-chemical parameters (i.e. temperature, pH, nutrients, H2S and N2O concentrations as well as the biodiversity and abundance of chemolithotrophic nitrate-reducing, sulfur-oxidizing γ-proteobacteria (SUP05 cluster using sequence analysis of amplified small subunit ribosomal RNA (16S rRNA genes in combination with taxon-specific quantitative polymerase chain reaction (qPCR assays. Denitrification was the dominant N-loss pathway in the subsurface biosphere of the Juan de Fuca Ridge, with rates of up to ~1000 nmol N l−1 day−1. In comparison, anammox rates were always <5 nmol N l−1 day−1 and below the detection limit at most of the sites. DNRA rates were up to 152 nmol N l−1 day−1. These results suggest that bacterial denitrification out-competes anammox in sulfidic hydrothermal vent waters. Taxon-specific qPCR revealed that γ-proteobacteria of the SUP05 cluster sometimes dominated the microbial community (SUP05/total bacteria up to 38%. Significant correlation existed between fixed N-loss (i.e., denitrification, anammox rates and in-situ nitrate and dissolved inorganic nitrogen (DIN deficits in the fluids, indicating that DIN availability may ultimately regulate N

  9. Hydrothermal liquefaction of cellulose to bio-oil under acidic, neutral and alkaline conditions

    International Nuclear Information System (INIS)

    Highlights: ► Hydrothermal liquefaction (HTL) at acidic, neutral and alkaline conditions. ► Bio-oil compositions varied with acidic, neutral and alkaline conditions. ► Reaction mechanisms varied with acidic, neutral and alkaline conditions. ► HTL should be classified to acidic, neutral and alkaline processes. -- Abstract: Hydrothermal liquefaction (HTL) of biomass to bio-oil under alkaline or neutral conditions has been widely reported in literature. However, there has been limited data available in literature on comparing HTL of biomass to bio-oil under acidic, neutral, and alkaline in terms of chemical compositions and yields by using the same reaction conditions and reactor. Using cellulose as a feedstock we conducted the comparative studies for pH = 3, 7 and 14 at temperatures of 275–320 °C with reaction residence times of 0–30 min. Results showed that the chemical compositions of the bio-oils were different for acidic, neutral and alkaline conditions. Under acidic and neutral conditions, the main composition of HTL bio-oil was 5-(Hydroxymethyl)furfural (HMF). Under alkaline conditions, the main compounds became C2–5 carboxylic acids. For bio-oil yields, it was observed that high temperatures and long residence times had negative effects, regardless of the pH levels. However, the corresponding reaction mechanisms are different. Under acidic conditions, the decrease in the bio-oil yields was mainly caused by polymerization of 5-HMF to solids. Under neutral conditions, the bio-oil yields decreased because 5-HMF was converted to both solid and gaseous products. Under alkaline conditions, the bio-oil decomposed to gases through the formation of short chain acids and aldehydes. Therefore, although they were all referred to as HTL bio-oil in literature, they were formed by different reaction pathways and had different properties due to their different chemical compositions. Given these differences, different strategies are recommended in this study to

  10. Microbial biofilms associated with fluid chemistry and megafaunal colonization at post-eruptive deep-sea hydrothermal vents

    Science.gov (United States)

    O'Brien, Charles E.; Giovannelli, Donato; Govenar, Breea; Luther, George W.; Lutz, Richard A.; Shank, Timothy M.; Vetriani, Costantino

    2015-11-01

    At deep-sea hydrothermal vents, reduced, super-heated hydrothermal fluids mix with cold, oxygenated seawater. This creates temperature and chemical gradients that support chemosynthetic primary production and a biomass-rich community of invertebrates. In late 2005/early 2006 an eruption occurred on the East Pacific Rise at 9°50‧N, 104°17‧W. Direct observations of the post-eruptive diffuse-flow vents indicated that the earliest colonizers were microbial biofilms. Two cruises in 2006 and 2007 allowed us to monitor and sample the early steps of ecosystem recovery. The main objective of this work was to characterize the composition of microbial biofilms in relation to the temperature and chemistry of the hydrothermal fluids and the observed patterns of megafaunal colonization. The area selected for this study had local seafloor habitats of active diffuse flow (in-flow) interrupted by adjacent habitats with no apparent expulsion of hydrothermal fluids (no-flow). The in-flow habitats were characterized by higher temperatures (1.6-25.2 °C) and H2S concentrations (up to 67.3 μM) than the no-flow habitats, and the microbial biofilms were dominated by chemosynthetic Epsilonproteobacteria. The no-flow habitats had much lower temperatures (1.2-5.2 °C) and H2S concentrations (0.3-2.9 μM), and Gammaproteobacteria dominated the biofilms. Siboglinid tubeworms colonized only in-flow habitats, while they were absent at the no-flow areas, suggesting a correlation between siboglinid tubeworm colonization, active hydrothermal flow, and the composition of chemosynthetic microbial biofilms.

  11. Microbial Sulfate Reduction in Deep-Sea Sediments at the Guaymas Basin - Hydrothermal Vent Area - Influence of Temperature and Substrates

    DEFF Research Database (Denmark)

    ELSGAARD, L.; ISAKSEN, MF; JØRGENSEN, BB; ALAYSE, AM; JANNASCH, HW

    1994-01-01

    -1 at 70-degrees-C. The rates of sulfate reduction rapidly decreased with depth in the upper 0- 10 cm of the sediment and the maximal depth-integrated rate (0-10 cm) was 70.3 mmol SO42- m-2 d-1 at 70-degrees-C. In comparison, the sulfate reduction rate in nonhydrothermal sediment from the vent area...... was 0.85 mmol m-2 d-1 at the in situ temperature of about 3-degrees-C. The high subsurface rates of sulfate reduction in the hydrothermal vent area was attributed to an enhanced local substrate availability. In slurries of hydrothermal sediment, incubated at 10-120-degrees-C, microbial sulfate....... Sulfate reduction was measured in intact cores of hydrothermal sediment at 3-degrees, 20-degrees, 35-degrees, 50-degrees, 70-degrees, and 90-degrees-C. The maximum rates of sulfate reduction were found in the upper 0-2 cm of the sediment and ranged from 32 nmol cm-3 d-1 at 90-degrees-C to 1563 nmol cm-3 d...

  12. Moytirra: Discovery of the first known deep-sea hydrothermal vent field on the slow-spreading Mid-Atlantic Ridge north of the Azores

    Science.gov (United States)

    Wheeler, A. J.; Murton, B.; Copley, J.; Lim, A.; Carlsson, J.; Collins, P.; Dorschel, B.; Green, D.; Judge, M.; Nye, V.; Benzie, J.; Antoniacomi, A.; Coughlan, M.; Morris, K.

    2013-10-01

    Geological, biological, morphological, and hydrochemical data are presented for the newly discovered Moytirra vent field at 45oN. This is the only high temperature hydrothermal vent known between the Azores and Iceland, in the North Atlantic and is located on a slow to ultraslow-spreading mid-ocean ridge uniquely situated on the 300 m high fault scarp of the eastern axial wall, 3.5 km from the axial volcanic ridge crest. Furthermore, the Moytirra vent field is, unusually for tectonically controlled hydrothermal vents systems, basalt hosted and perched midway up on the median valley wall and presumably heated by an off-axis magma chamber. The Moytirra vent field consists of an alignment of four sites of venting, three actively emitting "black smoke," producing a complex of chimneys and beehive diffusers. The largest chimney is 18 m tall and vigorously venting. The vent fauna described here are the only ones documented for the North Atlantic (Azores to Reykjanes Ridge) and significantly expands our knowledge of North Atlantic biodiversity. The surfaces of the vent chimneys are occupied by aggregations of gastropods (Peltospira sp.) and populations of alvinocaridid shrimp (Mirocaris sp. with Rimicaris sp. also present). Other fauna present include bythograeid crabs (Segonzacia sp.) and zoarcid fish (Pachycara sp.), but bathymodiolin mussels and actinostolid anemones were not observed in the vent field. The discovery of the Moytirra vent field therefore expands the known latitudinal distributions of several vent-endemic genera in the north Atlantic, and reveals faunal affinities with vents south of the Azores rather than north of Iceland.

  13. Influence of alkaline hydrothermal pretreatment on shrub wood Tamarix ramosissima: Characteristics of degraded lignin

    International Nuclear Information System (INIS)

    The objective of this work was to evaluate the influence of alkaline hydrothermal (AH) pretreatment on the physicochemical properties of the degraded lignins, attempt to upgrade the potential of lignin for value-added chemicals and fuel production. For this purpose, shrub wood Tamarix ramosissima lignin was fractionated using a two-stage process based on an AH pretreatment followed by an alkaline ethanol post-treatment. The recovered lignin fractions were investigated by comparison with milled wood lignin (MWL) in terms of fractionation yield, carbohydrate composition, gel permeation chromatography, Fourier transform infrared spectroscopy, 13C and 2D heteronuclear single quantum correlation nuclear magnetic resonance, as well as pyrolysis-gas chromatography/mass spectrometry. The result showed that AH pretreatment led to the degradation of β-O-4 linkages and consequently the increased severity caused a release of more S-units lignin fractions with molecular weights between 1300 and 2500 g/mol in the liquid but higher molecular weights (3000–4400 g/mol) in the residues. Moreover, it was found that the lignin syringyl-to-guaiacyl (S/G) ratios from analytical pyrolysis slightly changed after AH pretreatment (S/G, 1.8–2.3) but higher than those of MWL (S/G, 1.7). Overall, the present study demonstrates that these lignins dissolved during AH pretreatment and those recovered from the solid residues isolated with alkaline ethanol post-treatment could be profitably exploited as feedstock in integrated forest biorefineries, rather than traditional use as low-value energy sources.- Highlights: • Alkaline hydrothermal pretreatment and alkaline ethanol post-treatment were proposed. • The influence of AH pretreatment on the lignin structural changes was characterized. • Aryl-O-ether linkages of lignin were extensively cleaved. • Lignin recovered from solid residue is a potential resource for the production of value-added chemicals

  14. The Arctic Gakkel Vents (AGAVE) Expedition: Technology Development and the Search for Deep-Sea Hydrothermal Vent Fields Under the Arctic Ice Cap

    Science.gov (United States)

    Reves-Sohn, R. A.; Singh, H.; Humphris, S.; Shank, T.; Jakuba, M.; Kunz, C.; Murphy, C.; Willis, C.

    2007-12-01

    Deep-sea hydrothermal fields on the Gakkel Ridge beneath the Arctic ice cap provide perhaps the best terrestrial analogue for volcanically-hosted chemosynthetic biological communities that may exist beneath the ice-covered ocean of Europa. In both cases the key enabling technologies are robotic (untethered) vehicles that can swim freely under the ice and the supporting hardware and software. The development of robotic technology for deep- sea research beneath ice-covered oceans thus has relevance to both polar oceanography and future astrobiological missions to Europa. These considerations motivated a technology development effort under the auspices of NASA's ASTEP program and NSF's Office of Polar Programs that culminated in the AGAVE expedition aboard the icebreaker Oden from July 1 - August 10, 2007. The scientific objective was to study hydrothermal processes on the Gakkel Ridge, which is a key target for global studies of deep-sea vent fields. We developed two new autonomous underwater vehicles (AUVs) for the project, and deployed them to search for vent fields beneath the ice. We conducted eight AUV missions (four to completion) during the 40-day long expedition, which also included ship-based bathymetric surveys, CTD/rosette water column surveys, and wireline photographic and sampling surveys of remote sections of the Gakkel Ridge. The AUV missions, which lasted 16 hours on average and achieved operational depths of 4200 meters, returned sensor data that showed clear evidence of hydrothermal venting, but for a combination of technical reasons and time constraints, the AUVs did not ultimately return images of deep-sea vent fields. Nevertheless we used our wireline system to obtain images and samples of extensive microbial mats that covered fresh volcanic surfaces on a newly discovered set of volcanoes. The microbes appear to be living in regions where reducing and slightly warm fluids are seeping through cracks in the fresh volcanic terrain. These discoveries

  15. Radiometric dating of sediment cores from a hydrothermal vent zone off Milos Island in the Aegean Sea

    International Nuclear Information System (INIS)

    Sediment cores from a hydrothermal vent zone off Milos Island in the Aegean Sea were dated using the 210Pb method. The average unsupported 210Pb inventory in the cores was calculated to be 3256 Bq m-2. The corresponding mean annual 210Pb flux of 105 Bq m-2 year-1 is comparable to estimates of the atmospheric flux given in the literature. 210Pb fluxes calculated from the unsupported 210Pb inventories in cores are also comparable with the 210Pb vertical fluxes determined from settling particles off the coast of Milos Island. The highest unsupported 210Pb concentrations (89 Bq kg-1) were measured in the sediments nearest to the hydrothermal vent area suggesting that the sedimentation rate is lowest at this site. Direct gamma measurements of 210Pb were used to date three sediment cores that are located at different distances from the vent zone: one is in the immediate vicinity of the vent; and others are outside the zone. Sedimentation rates for these cores, calculated using the CRS and CIC models, ranged from 0.088±0.008 cm year-1 to 0.14±0.01 cm year-1. Where both models were applicable, the results given by the two methods were in good agreement. 137Cs concentrations in all three cores generally declined with depth but showed no clear signal of either the period of maximum fallout from weapons testing or the Chernobyl accident. 210Po activities were also measured and the maximum 210Po concentration was in the sediment surface layer (166 Bq kg-1)

  16. An Unusual Conformational Isomer of Verrucosidin Backbone from a Hydrothermal Vent Fungus, Penicillium sp. Y-50-10.

    Science.gov (United States)

    Pan, Chengqian; Shi, Yutong; Auckloo, Bibi Nazia; Chen, Xuegang; Chen, Chen-Tung Arthur; Tao, Xinyi; Wu, Bin

    2016-01-01

    A new verrucosidin derivative, methyl isoverrucosidinol (1), was isolated from the marine fungus Penicillium sp. Y-50-10, dwelling in sulfur rich sediment in the Kueishantao hydrothermal vents off Taiwan. The structure was established by spectroscopic means including HRMS and 2D-NMR spectroscopic analysis. The absolute configuration was defined mainly by comparison of quantum chemical TDDFT calculated and experimental ECD spectra. Among hitherto known compounds with a verrucosidine backbone isolated from natural resource, compound 1 represents the first example of a new conformational isomer of its skeleton, exhibiting antibiotic activity against Bacillus subtilis with MIC value 32 μg/mL. PMID:27548192

  17. An Unusual Conformational Isomer of Verrucosidin Backbone from a Hydrothermal Vent Fungus, Penicillium sp. Y-50-10

    Directory of Open Access Journals (Sweden)

    Chengqian Pan

    2016-08-01

    Full Text Available A new verrucosidin derivative, methyl isoverrucosidinol (1, was isolated from the marine fungus Penicillium sp. Y-50-10, dwelling in sulfur rich sediment in the Kueishantao hydrothermal vents off Taiwan. The structure was established by spectroscopic means including HRMS and 2D-NMR spectroscopic analysis. The absolute configuration was defined mainly by comparison of quantum chemical TDDFT calculated and experimental ECD spectra. Among hitherto known compounds with a verrucosidine backbone isolated from natural resource, compound 1 represents the first example of a new conformational isomer of its skeleton, exhibiting antibiotic activity against Bacillus subtilis with MIC value 32 μg/mL.

  18. Production of lactic acid from C6-polyols by alkaline hydrothermal reactions

    International Nuclear Information System (INIS)

    Production of lactic acid from C6-polyols (Mannitol) under alkaline hydrothermal conditions was investigated. Experiments were performed to examine the difference in the production of lactic acid between C6-polyols and C3-polyols (glycerine), as well as C6-aldoses (glucose). Results showed that the yield of lactic acid from C6-polyols was lower than that from both glycerine and glucose. It indicated that long chain polyols might follow a different reaction pathway from that of glycerine. Further investigation is needed to clarify the reaction mechanism and improve the relatively low lactic acid acid yield from C6-polyols.

  19. Hydrothermal and magmatic couplings at mid-ocean ridges : controls on the locations of high-temperature hydrothermal vent fields

    Science.gov (United States)

    Fontaine, Fabrice; Rabinowicz, Michel; Cannat, Mathilde; Escartin, Javier

    2013-04-01

    The heat output and thermal regime of oceanic spreading centers are strongly controlled by boundary layer processes between the hydrothermal system and the underlying crustal magma chamber, which remain to be fully understood. In thermal models, the dynamical interactions between the hydrothermal system and the deeper part of the lithosphere affected by processes such as magma chamber convection, magma crystallization and latent heat release, or simple conduction, is usually not considered and a ad-hoc temperature or heat flux is prescribed at the base of the hydrothermal layer. In this work we develop original two-dimensional numerical models of the interactions between a shallow cellular hydrothermal (porous) system at temperatures hydrothermal fields such as those discovered along the East Pacific Rise at 9°50'N or along the Juan de Fuca ridge/Endeavour segment for example. One hypothesis is that these fields require the formation of "elongated" hydrothermal convection cells that cool the crust on 5-10 kms, but the processes controlling the formation of such large aspect ratio (length/height) are poorly constrain. Our models show that such cells naturally arise from the dynamical coupling between a « low-viscosity », convecting lower-crust and a low-permeability upper hydrothermal layer. They also predict along-axis variations in the depth of the axial magma lens (AMC) seismic reflector of fast- and intermediate-spreading axes, and constrain lower oceanic crust cooling rates. They also suggest a dynamic link between upper oceanic crust along-axis permeability variations and stresses due to lower crust magmatic flow. We discuss the likelihood of this range of viscosity and permeability using available oceanic and ophiolites constraints and suggest that our work can motivate and help design future deep-sea experiments (e.g., drilling…).

  20. Free-living bacterial communities associated with tubeworm (Ridgeia piscesae) aggregations in contrasting diffuse flow hydrothermal vent habitats at the Main Endeavour Field, Juan de Fuca Ridge

    OpenAIRE

    Forget, Nathalie L; Kim Juniper, S

    2013-01-01

    We systematically studied free-living bacterial diversity within aggregations of the vestimentiferan tubeworm Ridgeia piscesae sampled from two contrasting flow regimes (High Flow and Low Flow) in the Endeavour Hydrothermal Vents Marine Protected Area (MPA) on the Juan de Fuca Ridge (Northeast Pacific). Eight samples of particulate detritus were recovered from paired tubeworm grabs from four vent sites. Most sequences (454 tag and Sanger methods) were affiliated to the Epsilonproteobacteria, ...

  1. Flow rate perturbations in a black smoker hydrothermal vent in response to a mid-ocean ridge earthquake swarm

    Science.gov (United States)

    Crone, Timothy J.; Wilcock, William S. D.; McDuff, Russell E.

    2010-03-01

    Although there is indirect evidence for strong connections between tectonic processes and mid-ocean ridge hydrothermal flow, there are no direct observations of these links, primarily because measuring flow in these systems is difficult. Here we use an optical analysis technique to obtain a 44 day record of flow rate changes in a black smoker vent in the Main Endeavour field of the Juan de Fuca Ridge. We show that variations in the flow rate coincide with an earthquake swarm observed using an ocean bottom seismometer array. These observations indicate that connections between tectonics and flow are indeed strong, that hydraulic connections within this hydrothermal system are long ranging, and that enhanced tidal pumping of fluids may be initiated by earthquake activity. Because the effects of the swarm cross over an intervening vent field, we infer that the upflow zones feeding this field are narrow. Using the time lag between the swarm onset and the flow rate changes we estimate that the bulk permeability of the crust on the Endeavour segment ranges from 3.0 × 10-13 m2 to 6.0 × 10-12 m2.

  2. Rapid Formation of 1D Titanate Nanotubes Using Alkaline Hydrothermal Treatment and Its Photocatalytic Performance

    Directory of Open Access Journals (Sweden)

    Chin Wei Lai

    2015-01-01

    Full Text Available One-dimensional (1D titanate nanotubes (TNT were successfully synthesized using alkaline hydrothermal treatment of commercial TiO2 nanopowders in a Teflon lined stainless steel autoclave at 150°C. The minimum time required for the formation of the titanate nanotubes was 9 h significantly. After the hydrothermal processing, the layered titanate was washed with acid and water in order to control the amount of Na+ ions remaining in the sample solutions. In this study, the effect of different reaction durations in a range of 3 h to 24 h on the formation of nanotubes was carried out. As the reaction duration is extended, the changes in structure from particle to tubular shapes of alkaline treated TiO2 were obtained via scanning electron microscope (SEM. Also, the significant impact on the phase transformation and crystal structure of TNT was characterized through XRD and Raman analysis. Indeed, the photocatalytic activity of TNT was investigated through the degradation of methyl orange aqueous solution under the ultraviolet light irradiation. As a result, TNT with reaction duration at 6 h has a better photocatalytic performance than other samples which was correlated to the higher crystallinity of the samples as shown in XRD patterns.

  3. Crustal magnetization and the subseafloor structure of the ASHES vent field, Axial Seamount, Juan de Fuca Ridge: Implications for the investigation of hydrothermal sites

    Science.gov (United States)

    Caratori Tontini, Fabio; Crone, Timothy J.; Ronde, Cornel E. J.; Fornari, Daniel J.; Kinsey, James C.; Mittelstaedt, Eric; Tivey, Maurice

    2016-06-01

    High-resolution geophysical data have been collected using the Autonomous Underwater Vehicle (AUV) Sentry over the ASHES (Axial Seamount Hydrothermal Emission Study) high-temperature (~348°C) vent field at Axial Seamount, on the Juan de Fuca Ridge. Multiple surveys were performed on a 3-D grid at different altitudes above the seafloor, providing an unprecedented view of magnetic data resolution as a function of altitude above the seafloor. Magnetic data derived near the seafloor show that the ASHES field is characterized by a zone of low magnetization, which can be explained by hydrothermal alteration of the host volcanic rocks. Surface manifestations of hydrothermal activity at the ASHES vent field are likely controlled by a combination of local faults and fractures and different lava morphologies near the seafloor. Three-dimensional inversion of the magnetic data provides evidence of a vertical, pipe-like upflow zone of the hydrothermal fluids with a vertical extent of ~100 m.

  4. Characterizing the plasticity of nitrogen metabolism by the host and symbionts of the hydrothermal vent chemoautotrophic symbioses Ridgeia piscesae.

    Science.gov (United States)

    Liao, Li; Wankel, Scott D; Wu, Min; Cavanaugh, Colleen M; Girguis, Peter R

    2014-03-01

    Chemoautotrophic symbionts of deep sea hydrothermal vent tubeworms are known to provide their hosts with all their primary nutrition. While studies have examined how chemoautotrophic symbionts provide the association with nitrogen, fewer have examined if symbiont nitrogen metabolism varies as a function of environmental conditions. Ridgeia piscesae tubeworms flourish at Northeastern Pacific vents, occupy a range of microhabitats, and exhibit a high degree of morphological plasticity [e.g. long-skinny (LS) and short-fat (SF) phenotypes] that may relate to environmental conditions. This plasticity affords an opportunity to examine whether symbiont nitrogen metabolism varies among host phenotypes. LS and SF R. piscesae were recovered from the Axial and Main Endeavour Field hydrothermal vents. Nitrate and ammonium were quantified in Ridgeia blood, and the expression of key nitrogen metabolism genes, as well as stable nitrogen isotope ratios, was quantified in host branchial plume and symbiont-containing tissues. Nitrate and ammonium were abundant in the blood of both phenotypes though environmental ammonium concentrations were, paradoxically, lowest among individuals with the highest blood ammonium. Assimilatory nitrate reductase transcripts were always below detection, though in both LS and SF R. piscesae symbionts, we observed elevated expression of dissimilatory nitrate reductase genes, as well as symbiont and host ammonium assimilation genes. Site-specific differences in expression, along with tissue stable isotope analyses, suggest that LS and SF Ridgeia symbionts are engaged in both dissimilatory nitrate reduction and ammonia assimilation to varying degrees. As such, it appears that environmental conditions -not host phenotype-primarily dictates symbiont nitrogen metabolism. PMID:24237389

  5. Microbially-Mediated Sulfur Oxidation in Diffuse Hydrothermal Vent Fluids at Axial Seamount, Juan de Fuca Ridge

    Science.gov (United States)

    Akerman, N. H.; Butterfield, D. A.; Huber, J. A.

    2011-12-01

    Diffusely venting hydrothermal fluids can act as a window to the subseafloor microbial environment, where chemically-reduced hydrothermal fluids mixing with oxygenated seawater in the shallow crust creates chemical disequilibria that chemotrophic microorganisms can exploit for energy gain. At Axial Seamount, an active deep-sea volcano located on the Juan de Fuca Ridge, sulfide concentrations have been measured as high as 5770 μM, and sulfide oxidation is quantitatively the most important chemical energy source for microbial metabolism. In addition, studies of microbial population structure indicate that diffuse fluids at Axial are dominated by putative sulfur- and sulfide-oxidizing bacteria belonging to the Epsilonproteobacteria. To further study this important microbial process, we surveyed diffuse vent samples from Axial over a range of temperature, pH, and sulfide concentrations for the presence and expression of sulfide-oxidizing bacteria using a functional gene approach. Dissolved oxygen concentrations decrease exponentially above 40°C and lower the potential for sulfide oxidation, so we identified six sites of different temperatures, two each in the low (RNA was also extracted from the same sites to examine in situ expression of soxB. Data from these analyses, along with quantification of the soxB gene abundance and expression using quantitative PCR, are currently being carried out. Together, this data set of soxB gene diversity, expression, and abundance along with geochemical data will allow us to quantitatively determine the functional dynamics of sulfide oxidation in the subseafloor at Axial Seamount. The molecular techniques developed in this project are also being applied to fluid samples collected from many of the same sites at Axial following the recent 2011 eruption, as well as fluid samples collected from new snowblower vents believed to be rich in sulfide-oxidizing bacteria.

  6. Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean

    OpenAIRE

    Thomassen, Jon Hestetun

    2009-01-01

    Sponges are known to frequently contain a great number of symbiotic organisms. Symbiosis is also a characteristic trait of vent and seep animals worldwide. Sponges in the deep sea family Cladorhizidae, otherwise also known for their surprising ability to capture invertebrate prey, are among the most common sponges found in proximity to vents, and in one reported case methanotrophic symbiotic bacteria have been described in a cladorhizid species at a mud volcano. The phylogenetic relationships...

  7. Spatial variation in the population structure and reproductive biology of Rimicaris hybisae (Caridea: Alvinocarididae at hydrothermal vents on the Mid-Cayman Spreading Centre.

    Directory of Open Access Journals (Sweden)

    Verity Nye

    Full Text Available The dynamics and microdistribution of faunal assemblages at hydrothermal vents often reflect the fine-scale spatial and temporal heterogeneity of the vent environment. This study examined the reproductive development and population structure of the caridean shrimp Rimicaris hybisae at the Beebe and Von Damm Vent Fields (Mid-Cayman Spreading Centre, Caribbean using spatially discrete samples collected in January 2012. Rimicaris hybisae is gonochoric and exhibits iteroparous reproduction. Oocyte size-frequency distributions (21-823 µm feret diameters varied significantly among samples. Embryo development was asynchronous among females, which may result in asynchronous larval release for the populations. Specimens of R. hybisae from the Von Damm Vent Field (2294 m were significantly larger than specimens from the Beebe Vent Field. Brooding females at Von Damm exhibited greater size-specific fecundity, possibly as a consequence of a non-linear relationship between fecundity and body size that was consistent across both vent fields. Samples collected from several locations at the Beebe Vent Field (4944-4972 m revealed spatial variability in the sex ratios, population structure, size, and development of oocytes and embryos of this mobile species. Samples from the Von Damm Vent Field and sample J2-613-24 from Beebe Woods exhibited the highest frequencies of ovigerous females and significantly female-biased sex ratios. Environmental variables within shrimp aggregations may influence the distribution of ovigerous females, resulting in a spatially heterogeneous pattern of reproductive development in R. hybisae, as found in other vent taxa.

  8. Distribution, activity and function of short-chain alkane degrading phylotypes in hydrothermal vent sediments

    Science.gov (United States)

    Adams, M. M.; Joye, S. B.; Hoarfrost, A.; Girguis, P. R.

    2012-12-01

    Global geochemical analyses suggest that C2-C4 short chain alkanes are a common component of the utilizable carbon pool in deep-sea sediments worldwide and have been found in diverse ecosystems. From a thermodynamic standpoint, the anaerobic microbial oxidation of these aliphatic hydrocarbons is more energetically yielding than the anaerobic oxidation of methane (AOM). Therefore, the preferential degradation of these hydrocarbons may compete with AOM for the use of oxidants such as sulfate, or other potential oxidants. Such processes could influence the fate of methane in the deep-sea. Sulfate-reducing bacteria (SRB) from hydrocarbon seep sediments of the Gulf of Mexico and Guaymas Basin have previously been enriched that anaerobically oxidize short chain alkanes to generate CO2 with the preferential utilization of 12C-enriched alkanes (Kniemeyer et al. 2007). Different temperature regimens along with multiple substrates were tested and a pure culture (deemed BuS5) was isolated from mesophilic enrichments with propane or n-butane as the sole carbon source. Through comparative sequence analysis, strain BuS5 was determined to cluster with the metabolically diverse Desulfosarcina / Desulfococcus cluster, which also contains the SRB found in consortia with anaerobic, methane-oxidizing archaea in seep sediments. Enrichments from a terrestrial, low temperature sulfidic hydrocarbon seep also corroborated that propane degradation occurred with most bacterial phylotypes surveyed belonging to the Deltaproteobacteria, particularly Desulfobacteraceae (Savage et al. 2011). To date, no microbes capable of ethane oxidation or anaerobic C2-C4 alkane oxidation at thermophilic temperature have been isolated. The sediment-covered, hydrothermal vent systems found at Middle Valley (Juan de Fuca Ridge, eastern Pacific Ocean) are a prime environment for investigating mesophilic to thermophilic anaerobic oxidation of short-chain alkanes, given the elevated temperatures and dissolved

  9. Lipid biomarkers of deep-sea hydrothermal vent polychaetes— Alvinella pompejana, A. caudata, Paralvinella grasslei and Hesiolyra bergii

    Science.gov (United States)

    Phleger, Charles F.; Nelson, Matthew M.; Groce, Ami K.; Craig Cary, S.; Coyne, Kathryn; Gibson, John A. E.; Nichols, Peter D.

    2005-12-01

    The lipid composition was determined for 5 species of polychaete annelids collected by the Deep Submergence Vehicle ALVIN from high temperature chimneys at the 2500 m depth hydrothermal vent field of the East Pacific Rise. These are the first lipid biomarker analyses reported for these hydrothermal vent polychaetes. Lipid content was low in all samples (1.6-35.9 mg g -1 wet mass) and was dominated by polar lipid (78-90% of total lipid) with 8-19% sterol (ST), and very low storage lipid (triacylglycerol and wax ester). Total polyunsaturated fatty acids (PUFA) were moderately high (22-31% of total fatty acids (FA)) with extremely low or no docosahexaenoic acid (DHA, 22:6(n-3)). Eicosapentaenoic acid (EPA, 20:5(n-3)) levels were 5-6% in Alvinella pompejana and A. caudata and 10.3-13.7% in an errantiate polychaete (likely Hesionidae) and Hesiolyra bergii. There were greater PUFA and a greater EPA/AA (AA is arachidonic acid, 20:4(n-6)) ratio in the anterior versus the posterior half of A. pompejana, which may correlate to the strong temperature gradient reported in its tube. Total nonmethylene interrupted diunsaturated fatty acids (NMID) were 4-9% of total FA for most polychaete species and included several 20:2 and 22:2 components. The principal monounsaturated fatty acids (MUFA) included 18:1(n-7)c (14-19%), 16:1(n-7)c (2.6-10%) and 20:1(n-11)c (3-7% of total FA). These polychaete species may desaturate and elongate the bacterial-derived 18:1(n-7)c to obtain the essential FA EPA and AA. The major ST in the polychaetes is cholesterol (89-98% of total ST) with less cholesterol in the gut contents of A. pompejana. Other ST included 24-ethylcholesterol (1.5-5% of total ST) with lesser amounts of 24-methylenecholesterol, desmosterol, lathosterol, 24-methylcholesterol, 24-ethylcholesterol, and the stanols dehydrocholestanol and cholestanol. The high ST levels could play a role in thermal adaptation of membranes at the hydrothermal vent environment. Differences in the FA

  10. Molecular Diversity and Activity of Methanogens in the Subseafloor at Deep-Sea Hydrothermal Vents of the Pacific Ocean (Invited)

    Science.gov (United States)

    Huber, J. A.; Merkel, A.; Holden, J. F.; Lilley, M. D.; Butterfield, D. A.

    2009-12-01

    Methanogenesis is thought to represent one of the most ancient metabolic pathways on Earth, and methanogens may serve as important primary producers in warm crustal habitats at deep-sea hydrothermal vents. Many of these obligate chemolithoautotrophs depend solely on geochemically-derived energy and carbon sources and grow at high temperatures under strictly anaerobic conditions. A combined geochemical and microbiological approach was used to determine the distribution and molecular diversity of methanogens in low temperature diffuse vent fluids from the Endeavour Segment R2K ISS site, as well as Axial Seamount and volcanoes of the Mariana Arc. Geochemical data from hot and adjacent warm diffuse vent fluids provided chemical indicators to guide sample selection for detailed polymerase chain reaction (PCR)-based analysis of the key enzyme for methane formation, methyl-coenzyme M reductase (mcrA), as well as archaeal 16S rRNA genes. At most Endeavour vent sites, hydrogen concentrations were too low to support hydrogenotrophic methanogensis directly and only one diffuse site, Easter Island, had a positive signal for the mcrA gene. These sequences were most closely related to members of the order Methanococcales, as well as anaerobic methane oxidizers (ANME-1). The presence of ANME, which are rarely found in non-sedimented marine environments, is another line of evidence supporting the occurrence of buried sediments at Endeavour. At Axial, a number of diffuse vents have strong chemical indicators of methanogenesis. Methanogenic communities were detected at 3 sites on the southeast side of the caldera: the northern end of the 1998 lava flow, the International District, and on the pre-1987 lava flow. Time series work at Marker 113 showed that in 4 different years over the last 6 years methanogenic communities are active and abundant, suggesting a stable anaerobic, warm subseafloor habitat. Results show that members of the order Methanococcales dominate at this site

  11. Zeta-Proteobacteria dominate the formation of microbial mats in low-temperature hydrothermal vents at Loihi Seamount

    Science.gov (United States)

    Rassa, A. C.; McAllister, S. M.; Safran, S. A.; Moyer, C. L.

    2007-12-01

    Loihi Seamount is Hawaii's youngest volcano and one of the earth's most active. Loihi is located 30 km SE of the big island of Hawaii and rises over 3000m above the sea floor and summits at 1100m below sea level. An eruption in 1996 of Loihi led to the formation of Pele's Pit, a 300 meter deep caldera. The current observations have revealed diffuse hydrothermal venting causing low to intermediate temperatures (10 to 65°C). The elevated temperatures, coupled with high concentrations of Fe(II) (ranging from 50 to 750 μM) support conditions allowing for extensive microbial mat formation. The focus of this study was to identify the colonizing populations of bacteria generated by the microbial mats at Loihi Seamount. Twenty-six microbial growth chambers were deployed and recovered after placement in the flow of hydrothermal vents for 3 to 8 days from within Loihi's caldera. Genomic DNA was extracted from samples and analyzed by Terminal-Restriction Fragment Length Polymorphism (T-RFLP) using eight restriction enzyme treatments to generate fingerprints from bacterial amplicons of small subunit rRNA genes (SSU rDNAs). Pearson product-moment coupled with UPGMA cluster analysis of these T-RFLP fingerprints showed that these communities bifurcated into two primary clusters. The first (Group 1) had an average vent effluent temperature of 44°C, and the second (Group 2) had an average vent effluent temperature of 64°C. Representative samples from within the two clusters (or groups) were chosen for further clone library and sequencing analysis. These libraries revealing a dominance of the recently discovered zeta- Proteobacteria in the lower temperature group (Group 1) indicating that they were the dominant colonizers of the microbial mats. These microaerophilic, obligately lithotrophic, Fe-oxidizing bacteria are most closely related to Mariprofundus ferrooxydans. The higher temperature group (Group 2) was dominated by epsilon- Proteobacteria primarily of the genus

  12. Metal concentration and structural changes in Corallina elongata (Corallinales, Rhodophyta) from hydrothermal vents

    International Nuclear Information System (INIS)

    Shallow-water hydrothermal activity is widely present at Azores archipelago. Organisms in such environments present great potential as sentinels of the effects derived from chronically exposure to increased temperature, metal concentrations and reduced pH. This study aimed to evaluate metal concentration in Corallina elongata collected at locations exposed and not exposed to shallow-water hydrothermal activity and evaluate changes in its calcareous structure. Elemental concentration was determined and morphometric analysis was performed by scanning electron microscopy. Thicker cell walls and a bleached appearance were observed on C. elongata specimens from the hydrothermally active location, as well as increased concentrations of elements associated to volcanic activity. This study reports on metal accumulation and morphometric changes in the calcareous structure of C. elongata from a hydrothermally active location, adding new data for further research on such habitats and communities, providing an insight on how coralline algae might be affected by ocean acidification.

  13. Immunomodulatory N-acyl Dopamine Glycosides from the Icelandic Marine Sponge Myxilla incrustans Collected at a Hydrothermal Vent Site

    DEFF Research Database (Denmark)

    Einarsdottir, Eydis; Liu, Hong Bing; Freysdottir, Jona;

    2016-01-01

    A chemical investigation of the sponge (Porifera) Myxilla incrustans collected from the unique submarine hydrothermal vent site Strytan, North of Iceland, revealed a novel family of closely related N-acyl dopamine glycosides. Three new compounds, myxillin A (1), B (2) and C (3), were isolated and...

  14. Microbial community structure and nitrogenase gene diversity of sediment from a deep-sea hydrothermal vent field on the Southwest Indian Ridge

    Institute of Scientific and Technical Information of China (English)

    WU Yuehong; CAO Yi; WANG Chunsheng; WU Min; AHARON Oren; XU Xuewei

    2014-01-01

    A sediment sample was collected from a deep-sea hydrothermal vent field located at a depth of 2 951 m on the Southwest Indian Ridge. Phylogenetic analyses were performed on the prokaryotic community using polymerase chain reaction (PCR) amplification of the 16S rRNA and nifH genes. Within the Archaea, the dominant clones were from marine benthic group E (MBGE) and marine group I (MGI) belonging to the phyla Euryarchaeota and Thaumarchaeota, respectively. More than half of the bacterial clones belonged to the Proteobacteria, and most fell within the Gammaproteobacteria. No epsilonproteobacterial sequence was observed. Additional phyla were detected including the Actinobacteria, Bacteroidetes, Planctomycetes, Acidobacteria, Nitrospirae, Chloroflexi, Chlorobi, Chlamydiae, Verrucomicrobia, and candidate divisions OD1, OP11, WS3 and TM6, confirming their existence in hydrothermal vent environments. The detection of nifH gene suggests that biological nitrogen fixation may occur in the hydrothermal vent field of the South-west Indian Ridge. Phylogenetic analysis indicated that only Clusters I and III NifH were present. This is consistent with the phylogenetic analysis of the microbial 16S rRNA genes, indicating that Bacteria play the main role in nitrogen fixation in this hydrothermal vent environment.

  15. Mid-Ocean Ridge Hydrothermal Vent Fluid Chemistry at Ultrafast Spreading Rates: Control by Phase Separation and Water-Rock Equilibrium

    Science.gov (United States)

    O'Grady, K. M.; Von Damm, K. L.

    2001-12-01

    Phase separation, overprinted by water-rock equilibration are the major controls on the chemical composition of hydrothermal vent fluids sampled from two morphologically distinct areas (18\\deg 24-26'S and 21\\deg 24-27'S) along the ultrafast spreading ( ~15 cm/yr full rate) Southern East Pacific Rise (SEPR) during the 1998 SouEPR Cruise. This conclusion, along with the growing evidence that phase separation and water-rock equilibrium also control the composition of previously sampled hydrothermal vent fluids from slower-spreading ridges, indicates that to a first approximation neither spreading rate nor ridge morphology can be directly related to hydrothermal fluid compositions. Hydrothermal fluids from ultrafast spreading centers therefore do not form a unique subset in the global range of known chemical compositions. Previous geophysical surveys and submersible observations suggested that the hydrothermal system located at 21\\deg 24-27'S, the SouEPR Area, was dominated by tectonic activity (Renard et al., 1985; Tufar, 1995; Krasnov et al., 1997). Submersible observations and hydrothermal vent fluid chemistry indicated that the N. Hump Area, experienced volcanic activity shortly before the 1993 NADUR Cruise (Charlou et al., 1996). The N. Hump Area vent fluids sampled during the 1998 SouEPR Cruise displayed a relatively uniform chlorinity (616-670 mmol/kg Cl) that is greater than seawater. The Si and Cl data from the N. Hump Area vent fluids suggest reaction zone conditions up to ~360 bars (~1 km below the seafloor) and ~430\\deg C, indicating supercritical phase separation. The unusually large chlorinity variation (113-803 mmol/kg Cl) in the SouEPR Area hydrothermal vent fluids covers almost the entire range of sampled mid-ocean ridge (MOR) hydrothermal vent fluid chemistries worldwide (30.5-1245 mmol/kg Cl). The Si and Cl data from the SouEPR Area vent fluids suggest reaction zone conditions up to ~410 bars ( ~1.3 km below the seafloor) and ~450\\deg C. The

  16. The hydrothermal alteration in the context of geologic evolution from Pocos de Caldas Alkaline Massif, MG-SP

    International Nuclear Information System (INIS)

    The Pocos de Caldas Alkaline Massif covers 800 km2, a quarter of which is hydrothermally altered. Such proportion is uncommon, when compared to the know alkaline massifs of the world. The hydrothermal alteration is associated with Zr, U and Mo mineralizations which are predominantly located in the central-southern portion of the massif, in the central-eastern circular structure. The colour of the altered rock (and its soil) in that area is typically whitish beige to yellowish white and is regionally called potassic rock. The Osamu Utsumi Mine, also referred to as the uranium ore of Campo do Cercado, is located 25 Km to the south of Pocos de Caldas City and was explored, from 1977 to 1989, through the open pit method. A sequence of alteration minerals adapted to lowering temperatures should be expected; however, only illite and alkaline feldspar are observed in the hydrothermally altered portions of the massif, and their formation must have been controlled mainly by kinetic, other than thermal factors. The irrestrict circulation of relatively hotter hydrothermal fluids must have happened at the beginning of the process, diminishing immediately after the cooling of the brecciated areas (and the subjacent magmatic body), leading the system to kinetics levels that made subsequent hydrothermal alteration impossible. (author)

  17. Isotopic signatures associated with growth and metabolic activities of chemosynthetic nitrate-reducing microbes from deep-sea hydrothermal vents

    Science.gov (United States)

    Perez-Rodriguez, I. M.; Foustoukos, D.; Fogel, M. L.; Sievert, S. M.

    2013-12-01

    Epsilonproteobacteria and Aquificaceae have been identified as dominant members of microbial communities at deep-sea hydrothermal vents. Cultured representatives from these two groups appear to be mostly genetically wired to perform chemosynthesis at moderate-to-high temperatures (45 - 80oC) under anaerobic and sulfidic conditions. In this study we used Caminibacter mediatlanticus and Thermovibrio ammonificans as model organisms to constrain physiological parameters associated with dissimilatory nitrate reduction to ammonium (DNRA) in deep-sea vent Epsilonproteobacteria and Aquificaceae. We postulate that nitrate-based metabolic processes are of relevance for understanding primary production as well as nitrate mobilization in deep-sea vents. By constraining growth and respiration rates during DNRA, we observed that C. mediatlanticus achieved higher cell densities than T. ammonificans while exhibiting similar growth rates. DNRA kinetic rate constants and cell-specific nitrate reduction rates (csNRR) obtained from our data showed that within similar time frames T. ammonificans used 2.5 to 3 times as much nitrate than C. mediatlanticus and it did so ~3 times faster. However, the increased consumption of nitrate in T. ammonificans did not translate into higher growth yield. This is suggestive of either differential efficiencies in energy generating pathways or differential organic matter production (cell biomass versus extracellular organic material) associated with DNRA in these microorganisms. Nitrogen isotope fractionation for nitrate was similar for both organisms, with discrimination factors of ~ -5 to -6‰ for C. mediatlanticus and ~ -7 to -8‰ for T. ammonificans. Similar experiments performed under high hydrostatic pressure conditions (50 and 200 bar) showed that changes in pressure greatly affected both growth rates and DNRA kinetic rate constants in both microorganisms, however, δ15N discrimination factors for nitrate were not affected. This study provides

  18. Deposition of talc - kerolite-smectite - smectite at seafloor hydrothermal vent fields: Evidence from mineralogical, geochemical and oxygen isotope studies

    Science.gov (United States)

    Dekov, V.M.; Cuadros, J.; Shanks, Wayne C.; Koski, R.A.

    2008-01-01

    controls on the precipitation of this sequence are the silica activity and Mg/Al ratio (i.e. the degree of mixing of seawater with hydrothermal fluid). Higher silica activity favors the formation of talc relative to tri-octahedral smectite. Vent structures and sedimentary cover preclude complete mixing of hydrothermal fluid and ambient seawater, resulting in lower Mg/Al ratios in the interior parts of the chimneys and deeper in the sediment which leads to the precipitation of phyllosilicates with lower Mg contents. Talc and kerolite-smectite have very low trace- and rare earth element contents. Some exhibit a negative or flat Eu anomaly, which suggests Eu depletion in the original hydrothermal fluid. Such Eu depletion could be caused by precipitation of anhydrite or barite (sinks for Eu2+) deeper in the system. REE abundances and distribution patterns indicate that chlorite and chlorite-smectite are hydrothermal alteration products of the background turbiditic sediment. ?? 2007 Elsevier B.V. All rights reserved.

  19. The production of glucose from corn stalk using hydrothermal process with pre-treatment ultrasound assisted alkaline

    Science.gov (United States)

    Yolanda, Dora; Prasutiyo, Indry; Trisanti, P. N.; Sumarno

    2015-12-01

    The production of glucose from corn stalk by using subcritical hydrothermal technology is studied in this work. Ultrasound-assisted alkaline delignification methods are used as pre-treatment. The corn stalk powder were pretreated with ultrasound-assisted alkaline (NaOH 2% w/w, solid to liquid ratio 1:22 w/v) at room temperature and 30 minutes. After pre-treatment, solid residue and liquid fractions are separated by filtration. Pretreated solids are further submitted to hydrothermal process for glucose production. Hydrothermal process was carried out at 100 Bar and 120°C in various times. The solid product was characterized by SEM and XRD. And liquid product was analysis using DNS method to determine percentage of glucose. From XRD analysis showed that crystallinity of material was lower than delignification product.

  20. Development of an ecotoxicological protocol for the deep-sea fauna using the hydrothermal vent shrimp Rimicaris exoculata.

    Science.gov (United States)

    Auguste, M; Mestre, N C; Rocha, T L; Cardoso, C; Cueff-Gauchard, V; Le Bloa, S; Cambon-Bonavita, M A; Shillito, B; Zbinden, M; Ravaux, J; Bebianno, M J

    2016-06-01

    In light of deep-sea mining industry development, particularly interested in massive-sulphide deposits enriched in metals with high commercial value, efforts are increasing to better understand potential environmental impacts to local fauna. The aim of this study was to assess the natural background levels of biomarkers in the hydrothermal vent shrimp Rimicaris exoculata and their responses to copper exposure at in situ pressure (30MPa) as well as the effects of depressurization and pressurization of the high-pressure aquarium IPOCAMP. R. exoculata were collected from the chimney walls of the hydrothermal vent site TAG (Mid Atlantic Ridge) at 3630m depth during the BICOSE cruise in 2014. Tissue metal accumulation was quantified in different tissues (gills, hepatopancreas and muscle) and a battery of biomarkers was measured: metal exposure (metallothioneins), oxidative stress (catalase, superoxide dismutase, glutathione-S-transferase and glutathione peroxidase) and oxidative damage (lipid peroxidation). Data show a higher concentration of Cu in the hepatopancreas and a slight increase in the gills after incubations (for both exposed groups). Significant induction of metallothioneins was observed in the gills of shrimps exposed to 4μM of Cu compared to the control group. Moreover, activities of enzymes were detected for the in situ group, showing a background protection against metal toxicity. Results suggest that the proposed method, including a physiologically critical step of pressurizing and depressurizing the test chamber to enable the seawater exchange during exposure to contaminants, is not affecting metal accumulation and biomarkers response and may prove a useful method to assess toxicity of contaminants in deep-sea species. PMID:27101410

  1. Hydrothermal synthesis of pure BaFe12O19 hexaferrite nanoplatelets under high alkaline system

    International Nuclear Information System (INIS)

    To our best knowledge, it is the first time to synthesize the single-phase BaFe12O19 hexaferrite by the single hydrothermal method at 220 °C. Meanwhile, effects of Fe/Ba ratio and KOH amount were also investigated. It was found that the proper Fe/Ba ratio could suppress the intermediate phase, such as α-Fe2O3 and BaFe2O4. One thing to be mentioned is that high alkaline system may be a key factor to prepare BaFe12O19 hexaferrite with even disk-like shape and narrow size distribution. The optimum Fe/Ba mole ratio is identified as 8:1, and the optimum dosage of KOH was 4.0 g for the preparation of single-phase BaFe12O19. Furthermore, the formation mechanism of disk-like BaFe12O19 hexaferrite was also discussed. - Highlights: ► Single-phase BaFe12O19 hexaferrite was synthesized by the single hydrothermal method. ► The only Fe/Ba mole ratio is identified as 8:1. ► The optimum amount of KOH was 4.0 g. ► Formation mechanism of disk-like shape for BaFe12O19 hexaferrite was discussed

  2. Alkaline hydrothermal synthesis of homogeneous titania microspheres with urchin-like nanoarchitectures for dye effluent treatments

    International Nuclear Information System (INIS)

    Highlights: → Alkali-hydrothermal treatments of a remnant of Ti-H2O2 reaction achieve titania microspheres. → Inhibited heterogeneous nucleation and low supersaturation contribute to the uniform size. → Radially aligned anatase nanowires construct the microspheres. → The microspheres possess a BET surface area of 45.4 m2/g. → The microspheres exhibit a high activity to assist photodegradation of rhodamine B in water. - Abstract: The heterogeneous photocatalysis technique to treat dye effluents demands micrometer-sized titania aggregates with one-dimensional nanostructures, which possess high photocatalytic activity and at the same time facilitate the catalyst-recovery from a slurry system. In this study, the solution remained after interactions between metallic Ti and hydrogen peroxide was subjected to an alkaline hydrothermal treatment. Microspheres with extremely uniform sizes of ca. 2 μm in diameter were achieved after a subsequent proton exchange followed by calcination in air. The microspheres were urchin-like aggregates of radially assembled nanowires, which consisted of chain-like anatase single crystallites with an average diameter of 20-25 nm. The homogeneous microspheres calcinated at 600 oC possessed a surface area of 45.4 m2/g and exhibited an excellent activity to assist photodegradation of rhodamine B in water, which is significantly higher than that of P25 titania nanoparticles. Because of the much easier recovery of the photocatalyst, the homogeneous microspheres synthesized herein may find practical applications in efficient photocatalytic treatments of dye effluents.

  3. Gamma- and epsilonproteobacterial ectosymbionts of a shallow-water marine worm are related to deep-sea hydrothermal vent ectosymbionts.

    Science.gov (United States)

    Ruehland, Caroline; Dubilier, Nicole

    2010-08-01

    The marine oligochaete worm Tubificoides benedii is often found in high numbers in eutrophic coastal sediments with low oxygen and high sulfide concentrations. A dense biofilm of filamentous bacteria on the worm's tail end were morphologically described over 20 years ago, but no further studies of these epibiotic associations were done. In this study, we used fluorescence in situ hybridization and comparative sequence analysis of 16S rRNA and protein-coding genes to characterize the microbial community of the worm's tail ends. The presence of genes involved in chemoautotrophy (cbbL and cbbM) and sulfur metabolism (aprA) indicated the potential of the T. benedii microbial community for chemosynthesis. Two filamentous ectosymbionts were specific to the worm's tail ends: one belonged to the Leucothrix mucor clade within the Gammaproteobacteria and the other to the Thiovulgaceae within the Epsilonproteobacteria. Both T. benedii ectosymbionts belonged to clades that consisted almost exclusively of bacteria associated with invertebrates from deep-sea hydrothermal vents. Such close relationships between symbionts from shallow-water and deep-sea hosts that are not closely related to each other are unusual, and indicate that biogeography and host affiliation did not play a role in these associations. Instead, similarities between the dynamic environments of vents and organic-rich mudflats with their strong fluctuations in reductants and oxidants may have been the driving force behind the establishment and evolution of these symbioses. PMID:21966922

  4. Finding immune gene expression differences induced by marine bacterial pathogens in the Deep-sea hydrothermal vent mussel Bathymodiolus azoricus

    Science.gov (United States)

    Martins, E.; Queiroz, A.; Serrão Santos, R.; Bettencourt, R.

    2013-11-01

    The deep-sea hydrothermal vent mussel Bathymodiolus azoricus lives in a natural environment characterised by extreme conditions of hydrostatic pressure, temperature, pH, high concentrations of heavy metals, methane and hydrogen sulphide. The deep-sea vent biological systems represent thus the opportunity to study and provide new insights into the basic physiological principles that govern the defense mechanisms in vent animals and to understand how they cope with microbial infections. Hence, the importance of understanding this animal's innate defense mechanisms, by examining its differential immune gene expressions toward different pathogenic agents. In the present study, B. azoricus mussels were infected with single suspensions of marine bacterial pathogens, consisting of Vibrio splendidus, Vibrio alginolyticus, or Vibrio anguillarum, and a pool of these Vibrio bacteria. Flavobacterium suspensions were also used as a non-pathogenic bacterium. Gene expression analyses were carried out using gill samples from infected animals by means of quantitative-Polymerase Chain Reaction aimed at targeting several immune genes. We also performed SDS-PAGE protein analyses from the same gill tissues. We concluded that there are different levels of immune gene expression between the 12 h to 24 h exposure times to various bacterial suspensions. Our results from qPCR demonstrated a general pattern of gene expression, decreasing from 12 h over 24 h post-infection. Among the bacteria tested, Flavobacterium is the bacterium inducing the highest gene expression level in 12 h post-infections animals. The 24 h infected animals revealed, however, greater gene expression levels, using V. splendidus as the infectious agent. The SDS-PAGE analysis also pointed at protein profile differences between 12 h and 24 h, particularly evident for proteins of 18-20 KDa molecular mass, where most dissimilarity was found. Multivariate analyses demonstrated that immune genes, as well as experimental

  5. Structural elucidation of sorghum lignins from an integrated biorefinery process based on hydrothermal and alkaline treatments.

    Science.gov (United States)

    Sun, Shao-Long; Wen, Jia-Long; Ma, Ming-Guo; Sun, Run-Cang

    2014-08-13

    An integrated process based on hydrothermal pretreatment (HTP) (i.e., 110-230 °C, 0.5-2.0 h) and alkaline post-treatment (2% NaOH at 90 °C for 2.0 h) has been performed for the production of xylooligosaccharide, lignin, and digestible substrate from sweet sorghum stems. The yield, purity, dissociation mechanisms, structural features, and structural transformations of alkali lignins obtained from the integrated process were investigated. It was found that the HTP process facilitated the subsequent alkaline delignification, releasing lignin with the highest yield (79.3%) and purity from the HTP residue obtained at 190 °C for 0.5 h. All of the results indicated that the cleavage of the β-O-4 linkages and degradation of β-β and β-5 linkages occurred under the harsh HTP conditions. Depolymerization and condensation reactions simultaneously occurred at higher temperatures (≥ 170 °C). Moreover, the thermostability of lignin was positively related to its molecular weight, but was also affected by the inherent structures, such as β-O-4 linkages and condensed units. These findings will enhance the understanding of structural transformations of the lignins during the integrated process and maximize the potential utilizations of the lignins in a current biorefinery process. PMID:25090032

  6. Coupled RNA-SIP and metatranscriptomics of active chemolithoautotrophic communities at a deep-sea hydrothermal vent.

    Science.gov (United States)

    Fortunato, Caroline S; Huber, Julie A

    2016-08-01

    The chemolithoautotrophic microbial community of the rocky subseafloor potentially provides a large amount of organic carbon to the deep ocean, yet our understanding of the activity and metabolic complexity of subseafloor organisms remains poorly described. A combination of metagenomic, metatranscriptomic, and RNA stable isotope probing (RNA-SIP) analyses were used to identify the metabolic potential, expression patterns, and active autotrophic bacteria and archaea and their pathways present in low-temperature hydrothermal fluids from Axial Seamount, an active submarine volcano. Metagenomic and metatranscriptomic results showed the presence of genes and transcripts for sulfur, hydrogen, and ammonium oxidation, oxygen respiration, denitrification, and methanogenesis, as well as multiple carbon fixation pathways. In RNA-SIP experiments across a range of temperatures under reducing conditions, the enriched (13)C fractions showed differences in taxonomic and functional diversity. At 30 °C and 55 °C, Epsilonproteobacteria were dominant, oxidizing hydrogen and primarily reducing nitrate. Methanogenic archaea were also present at 55 °C, and were the only autotrophs present at 80 °C. Correspondingly, the predominant CO2 fixation pathways changed from the reductive tricarboxylic acid (rTCA) cycle to the reductive acetyl-CoA pathway with increasing temperature. By coupling RNA-SIP with meta-omics, this study demonstrates the presence and activity of distinct chemolithoautotrophic communities across a thermal gradient of a deep-sea hydrothermal vent. PMID:26872039

  7. Microbial Diversity Associated with High Temperature Sulfide Deposits Along the East Pacific Rise Deep-Sea Hydrothermal Vents

    Science.gov (United States)

    Brooks, D.; Hoek, J.; Banta, A. B.; von Damm, K.; Reysenbach, A.

    2002-12-01

    In January 2002, hydrothermal chimneys were collected using DSV Alvin from active vents from 9° 17N to 9° 50N and 20° 49N to 20° 50N. Hydrothermal fluids were collected for end member chemistry prior to collecting the sulfide deposits. Chimney samples were sub-sectioned, separating surface and associated biofilm samples from inner chimney samples. Microbial diversity of sub-samples was assessed using culture-dependent and culturing-independent small subunit (16S) ribosomal RNA-based techniques. Initial bacterial diversity assessments using denaturing gradient gel electrophoresis (DGGE) support the global prevalence of epsilon Proteobacteria associated with deep-sea sulfide structures. These are closely related to the sulfur-reducing isolate Nautilia lithotrophica. The biofilm communities varied in complexity with one sample containing several 16S rRNA sequences (phylotypes, OTU's) of alpha, epsilon, and gamma Proteobacteria while others contained a single phylotype of epsilon Proteobacteria. One proteobacterial phylotype was present in all but one of the surface samples, and this sample contained unique alpha and epsilon proteobacterial sequences. The inner chimney samples lacked the most common epsilon proteobacterial 16S rRNA sequences. Enrichment culturing was restricted to selecting for thermophilic chemolithoautotrophic hydrogen-oxidizing Bacteria. The widespread distribution of Persephonella spp. was confirmed, and novel enrichments of a sheathed and as yet unidentified chemolithotroph were obtained.

  8. New records on sea anemones (Anthozoa: Actiniaria) from hydrothermal vents and cold seeps

    NARCIS (Netherlands)

    López-González, P.J.; Rodríguez, E.; Gili, J.-M.; Segonzac, M.

    2003-01-01

    During several cruises carried out by the Ifremer (Institut français de recherche pour l’exploitation de la mer) with the submersile “Nautile” at different hydrothermal sites and cold seeps, an important collection of anthozoans - mainly actiniarians - was sampled. Additional material was collected

  9. Structural and functional diversity of microbial communities beneath the hydrothermal vent at the Iheya North field of the Mid-Okinawa Trough (IODP Expedition 331)

    Science.gov (United States)

    Yanagawa, K.; Nunoura, T.; Kawagucci, S.; Hirai, M.; Sunamura, M.; Breuker, A.; Brandt, L.; House, C. H.; McAllister, S. M.; Moyer, C. L.; Takai, K.

    2012-12-01

    Complex and diverse microbial communities in deep-sea hydrothermal fluids are apparently different from those in ambient seawater, some of which are predicted to migrate along hydrothermal vein from "subvent biosphere". Subseafloor environment just beneath active hydrothermal vent has been expected to be one of the most conceivable habitats for metabolically active and diverse microbial community. We conducted the scientific ocean drilling (IODP Expedition 331) for the Iheya North hydrothermal field in the Mid-Okinawa Trough in Sept. 2010, and collected core samples from the subseafloor biosphere beneath the hydrothermal vent. IODP Site C0014 was located 450 m east off the main hydrothermal vent. Temperature exceeded the limit of life at the depth of approximately 40 m below the seafloor. Both microscopy and quantitative PCR analyses successfully detected microbial populations in the shallower zone above 15 mbsf. However, the cultivation attempts of (hyper-)thermophiles were unsuccessful all over the depth. Culture-independent molecular biological experiments showed that microbial community composition distinctly changed with depth, possibly because of physicochemical conditions such as methane, sulfate and temperature. Microbial activities of methanogenesis and anaerobic methane oxidation were in accordance with the geochemical profiles of methane and sulfate. These results indicated the presence of functionally active subseafloor microbial communities but those were different from expected members in subvent biosphere. Site C0017 located 1.6 km east off the hydrothermal vent is a potential seawater recharge zone of the hydrothermal system, where seawater penetrates into the oceanic crust. The lithostratigraphy consists of characteristic coarse angular pumiceous gravel, lying above and below hemipelagic mud, in which high permeability may allow entrainment of seawater. As is the case with sedimentary subsurface environments, uncultivated archaeal groups were

  10. Formation of Zn- and Fe-sulfides near hydrothermal vents at the Eastern Lau Spreading Center: implications for sulfide bioavailability to chemoautotrophs

    Directory of Open Access Journals (Sweden)

    Yucel Mustafa

    2008-05-01

    Full Text Available Abstract Background The speciation of dissolved sulfide in the water immediately surrounding deep-ocean hydrothermal vents is critical to chemoautotrophic organisms that are the primary producers of these ecosystems. The objective of this research was to identify the role of Zn and Fe for controlling the speciation of sulfide in the hydrothermal vent fields at the Eastern Lau Spreading Center (ELSC in the southern Pacific Ocean. Compared to other well-studied hydrothermal systems in the Pacific, the ELSC is notable for unique ridge characteristics and gradients over short distances along the north-south ridge axis. Results In June 2005, diffuse-flow ( 250°C vent fluids were collected from four field sites along the ELSC ridge axis. Total and filtered Zn and Fe concentrations were quantified in the vent fluid samples using voltammetric and spectrometric analyses. The results indicated north-to-south variability in vent fluid composition. In the high temperature vent fluids, the ratio of total Fe to total Zn varied from 39 at Kilo Moana, the most northern site, to less than 7 at the other three sites. The concentrations of total Zn, Fe, and acid-volatile sulfide indicated that oversaturation and precipitation of sphalerite (ZnS(s and pyrite (FeS2(s were possible during cooling of the vent fluids as they mixed with the surrounding seawater. In contrast, most samples were undersaturated with respect to mackinawite (FeS(s. The reactivity of Zn(II in the filtered samples was tested by adding Cu(II to the samples to induce metal-exchange reactions. In a portion of the samples, the concentration of labile Zn2+ increased after the addition of Cu(II, indicating the presence of strongly-bound Zn(II species such as ZnS clusters and nanoparticles. Conclusion Results of this study suggest that Zn is important to sulfide speciation at ELSC vent habitats, particularly at the southern sites where Zn concentrations increase relative to Fe. As the hydrothermal

  11. Post-eruption succession of macrofaunal communities at diffuse flow hydrothermal vents on Axial Volcano, Juan de Fuca Ridge, Northeast Pacific

    Science.gov (United States)

    Marcus, Jean; Tunnicliffe, Verena; Butterfield, David A.

    2009-09-01

    Hydrothermal vents harbor dense aggregations of invertebrate fauna supported by chemosynthesis. Severe tectonic events and volcanic eruptions frequently destroy vent communities and initiate primary succession at new vents on ridge-crest submarine lava flows. An eruption on Axial Volcano (˜1500 m depth), a seamount on the Juan de Fuca Ridge (JdFR) in the northeast Pacific Ocean, occurred in January 1998, which created new substratum and vents. This study examines the development of the macrofaunal vent assemblages associated with tubeworms ( Ridgeia piscesae) at eight diffuse flow vents over the following 3 years. Biological collections by suction of lava surfaces also characterized "pre-tubeworm assemblages". Coupled fluid sampling showed an overall decrease in temperature, sulphide, and sulphide-to-heat ratios over 3 years as well as large spatial variability across the new vents. We examined collections of pre-eruption diffuse flow vent assemblages at Axial Volcano to compare the stages of new community development to "mature" vents. Mature vent assemblages are characterized by two major community types dominated by limpets ( Lepetodrilus fucensis) and alvinellid polychaetes ( Paralvinella pandorae and/or P. palmiformis). The following post-eruption succession patterns emerged. First, R. piscesae tubeworms took up to 3 years to establish aggregations at the new vents, and the majority of pre-tubeworm assemblages were dominated by grazing polychaetes. Second, species colonized quickly and by 30 months after habitat creation >60% of Axial's species pool had arrived at the new vents; abundance at mature vents predicted colonization success with some notable exceptions. Third, shifts in species dominance occurred rapidly and by Year 3 new vent assemblages resembled mature, pre-eruption communities. In general, tubeworm assemblages were dominated by alvinellid polychaetes ( P. pandorae and P. palmiformis) in the first 2 years post-eruption, with limpets ( L

  12. Vitellibacter nionensis sp. nov., isolated from shallow water hydrothermal vent of Espalamaca, Azores.

    Digital Repository Service at National Institute of Oceanography (India)

    Rajasabapathy, R.; Mohandass, C.; Yoon, J.-H.; Dastager, S.G.; Liu, Q.; Khieu, T.-N.; Son, C.K.; Li, W.-J.; Colaco, A.

    5School of Biotechnology and Food Technology, Hanoi University of Science and Technology, Vietnam 6IMAR-Centre of IMAR of the University of the Azores/ Department of Oceanography and Fisheries and LARSyS Associated Laboratory, Rua Prof Frederico... concentration of methane, temperature 35 °C and pH 5.7 (Colaço, personal communication). Bottom water sample from the venting area was collected in September 2010 by scuba diving under Indo-Portugal bilateral program. For isolation of heterotrophic bacteria...

  13. Alkaline hydrothermal synthesis of homogeneous titania microspheres with urchin-like nanoarchitectures for dye effluent treatments

    Energy Technology Data Exchange (ETDEWEB)

    Wu, Jin-Ming, E-mail: msewjm@zju.edu.cn [State Key Laboratory of Silicon Materials, Zhejiang University, ZheDa Road 38, Hangzhou 310027 (China); Song, Xiao-Mei [State Key Laboratory of Silicon Materials, Zhejiang University, ZheDa Road 38, Hangzhou 310027 (China); Yan, Mi, E-mail: mse_yanmi@zju.edu.cn [Department of Materials Science and Engineering, Zhejiang University, ZheDa Road 38, Hangzhou 310027 (China)

    2011-10-30

    Highlights: {yields} Alkali-hydrothermal treatments of a remnant of Ti-H{sub 2}O{sub 2} reaction achieve titania microspheres. {yields} Inhibited heterogeneous nucleation and low supersaturation contribute to the uniform size. {yields} Radially aligned anatase nanowires construct the microspheres. {yields} The microspheres possess a BET surface area of 45.4 m{sup 2}/g. {yields} The microspheres exhibit a high activity to assist photodegradation of rhodamine B in water. - Abstract: The heterogeneous photocatalysis technique to treat dye effluents demands micrometer-sized titania aggregates with one-dimensional nanostructures, which possess high photocatalytic activity and at the same time facilitate the catalyst-recovery from a slurry system. In this study, the solution remained after interactions between metallic Ti and hydrogen peroxide was subjected to an alkaline hydrothermal treatment. Microspheres with extremely uniform sizes of ca. 2 {mu}m in diameter were achieved after a subsequent proton exchange followed by calcination in air. The microspheres were urchin-like aggregates of radially assembled nanowires, which consisted of chain-like anatase single crystallites with an average diameter of 20-25 nm. The homogeneous microspheres calcinated at 600 {sup o}C possessed a surface area of 45.4 m{sup 2}/g and exhibited an excellent activity to assist photodegradation of rhodamine B in water, which is significantly higher than that of P25 titania nanoparticles. Because of the much easier recovery of the photocatalyst, the homogeneous microspheres synthesized herein may find practical applications in efficient photocatalytic treatments of dye effluents.

  14. Microbial Community of a Hydrothermal Mud Vent Underneath the Deep-Sea Anoxic Brine Lake Urania (Eastern Mediterranean)

    Science.gov (United States)

    Yakimov, Michail M.; Giuliano, Laura; Cappello, Simone; Denaro, Renata; Golyshin, Peter N.

    2007-04-01

    The composition of a metabolically active prokaryotic community thriving in hydrothermal mud fluids of the deep-sea hypersaline anoxic Western Urania Basin was characterized using rRNA-based phylogenetic analysis of a clone library. The physiologically active prokaryotic assemblage in this extreme environment showed a great genetic diversity. Most members of the microbial community appeared to be affiliated to yet uncultured organisms from similar ecosystems, i.e., deep-sea hypersaline basins and hydrothermal vents. The bacterial clone library was dominated by phylotypes affiliated with the epsilon- Proteobacteria subdivision recognized as an ecologically significant group of bacteria inhabiting deep-sea hydrothermal environments. Almost 18% of all bacterial clones were related to delta- Proteobacteria, suggesting that sulfate reduction is one of the dominant metabolic processes occurring in warm mud fluids. The remaining bacterial phylotypes were related to alpha- and beta- Proteobacteria, Actinobacteria, Bacteroides, Deinococcus-Thermus, KB1 and OP-11 candidate divisions. Moreover, a novel monophyletic clade, deeply branched with unaffiliated 16S rDNA clones was also retrieved from deep-sea sediments and halocline of Urania Basin. Archaeal diversity was much lower and detected phylotypes included organisms affiliated exclusively with the Euryarchaeota. More than 96% of the archaeal clones belonged to the MSBL-1 candidate order recently found in hypersaline anoxic environments, such as endoevaporitic microbial mats, Mediterranean deep-sea mud volcanoes and anoxic basins. Two phylotypes, represented by single clones were related to uncultured groups DHVE-1 and ANME-1. Thus, the hydrothermal mud of hypersaline Urania Basin seems to contain new microbial diversity. The prokaryotic community was significantly different from that occurring in the upper layers of the Urania Basin since 60% of all bacterial and 40% of all archaeal phylotypes were obtained only from mud

  15. Microbial communities and chemosynthesis in yellowstone lake sublacustrine hydrothermal vent waters.

    Science.gov (United States)

    Yang, Tingting; Lyons, Shawn; Aguilar, Carmen; Cuhel, Russell; Teske, Andreas

    2011-01-01

    Five sublacustrine thermal spring locations from 1 to 109 m water depth in Yellowstone Lake were surveyed by 16S ribosomal RNA gene sequencing in relation to their chemical composition and dark CO(2) fixation rates. They harbor distinct chemosynthetic bacterial communities, depending on temperature (16-110°C) and electron donor supply (H(2)S 100 μM; NH(3) 10 μM). Members of the Aquificales, most closely affiliated with the genus Sulfurihydrogenibium, are the most frequently recovered bacterial 16S rRNA gene phylotypes in the hottest samples; the detection of these thermophilic sulfur-oxidizing autotrophs coincided with maximal dark CO(2) fixation rates reaching near 9 μM C h(-1) at temperatures of 50-60°C. Vents at lower temperatures yielded mostly phylotypes related to the mesophilic gammaproteobacterial sulfur oxidizer Thiovirga. In contrast, cool vent water with low chemosynthetic activity yielded predominantly phylotypes related to freshwater Actinobacterial clusters with a cosmopolitan distribution. PMID:21716640

  16. Presence and diversity of anammox bacteria in cold hydrocarbon-rich seeps and hydrothermal vent sediments of the Guaymas Basin

    Directory of Open Access Journals (Sweden)

    Lina eRuss

    2013-08-01

    Full Text Available Hydrothermally active sediments are highly productive, chemosynthetic areas which are characterized by the rapid turnover of particulate organic matter under extreme conditions in which ammonia is liberated. These systems might be suitable habitats for anaerobic ammonium oxidizing (anammox bacteria but this has not been investigated in detail. Here we report the diversity and abundance of anammox bacteria in sediments that seep cold hydrocarbon-rich fluids and hydrothermal vent areas of the Guaymas Basin in the Cortés Sea using the unique functional anammox marker gene, hydrazine synthase (hzsA. All clones retrieved were closely associated to the ‘Candidatus Scalindua’ genus. Phylogenetic analysis revealed two distinct clusters of hzsA sequences (Ca. Scalindua hzsA cluster I and II. Comparison of individual sequences from both clusters showed that several of these sequences had a similarity as low as 76% on nucleotide level. Based on the analysis of this phylomarker, a very high interspecies diversity within the marine anammox group is apparent. Absolute numbers of anammox bacteria in the sediments samples were determined by amplification of a 257 bp fragment of the hszA gene in a qPCR assay. The results indicate that numbers of anammox bacteria are generally higher in cold hydrocarbon-rich sediments compared to the vent areas and the reference zone. Ladderanes, lipids unique to anammox bacteria were also detected in several of the sediment samples corroborating the hzsA analysis. Due to the high concentrations of reduced sulfur compounds and its potential impact on the cycling of nitrogen we aimed to get an indication about the key players in the oxidation of sulfide in the Guaymas Basin sediments using the alpha subunit of the adenosine-5’-phosphosulfate (APS reductase (aprA. Amplification of the aprA gene revealed a high number of gammaproteobacterial aprA genes covering the two sulfur-oxidizing bacteria aprA lineages as well as

  17. Finding immune gene expression differences induced by marine bacterial pathogens in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

    Directory of Open Access Journals (Sweden)

    R. Bettencourt

    2013-02-01

    Full Text Available The deep-sea hydrothermal vent mussel Bathymodiolus azoricus lives in a natural environment characterized by extreme conditions of hydrostatic pressure, temperature, pH, high concentrations of heavy metals, methane and hydrogen sulphide. The deep-sea vent biological systems represent thus the opportunity to study and provide new insights into the basic physiological principles that govern the defense mechanisms in vent animals and to understand how they cope with microbial infections. Hence, the importance of understanding this animal's innate defense mechanisms, by examining its differential immune gene expressions toward different pathogenic agents. In the present study, B. azoricus mussels were infected with single suspensions of marine bacterial pathogens, consisting of Vibrio splendidus, Vibrio alginolyticus, or Vibrio anguillarum, and a pool of these Vibrio strains. Flavobacterium suspensions were also used as an irrelevant bacterium. Gene expression analyses were carried out using gill samples from animals dissected at 12 h and 24 h post-infection times by means of quantitative-Polymerase Chain Reaction aimed at targeting several immune genes. We also performed SDS-PAGE protein analyses from the same gill tissues. We concluded that there are different levels of immune gene expression between the 12 h and 24 h exposure times to various bacterial suspensions. Our results from qPCR demonstrated a general pattern of gene expression, decreasing from 12 h over 24 h post-infection. Among the bacteria tested, Flavobacterium is the microorganism species inducing the highest gene expression level in 12 h post-infections animals. The 24 h infected animals revealed, however, greater gene expression levels, using V. splendidus as the infectious agent. The SDS-PAGE analysis also pointed at protein profile differences between 12 h and 24 h, particularly around a protein area, of 18 KDa molecular mass, where most dissimilarities were found. Multivariate

  18. Time Series Measurements of Diffuse Hydrothermal Flow at the ASHES Vent Field Reveal Tidally Modulated Heat and Volume Flux

    Science.gov (United States)

    Mittelstaedt, E. L.; Fornari, D. J.; Crone, T. J.

    2015-12-01

    Existing time-series measurements of temperature and velocity of diffuse hydrothermal fluids exhibit variability over a range of periods from seconds to days. Frequency analysis of these measurements reveals differences between studies and field locations including nearly white spectra, as well as spectra with peaks at tidal and inertial periods. Based upon these results, previous authors have suggested several processes that may control diffuse flow rates, including tidally induced currents and 'tidal pumping', and have also suggested that there are no systematic controls. To further investigate the processes that control variability in diffuse flow, we use data from a new, deep-sea camera and temperature measurement system, the Diffuse Effluent Measurement System (DEMS), deployed during the July, 2014 cruise of the R/V Atlantis. The DEMS was deployed with DSV Alvin above a fracture network at the Phoenix vent within the ASHES vent field (Axial Seamount, 1541 mbsl). The system collected 20 seconds of imagery at 20 Hz and 24 seconds of temperature measurements at 1 Hz each hour over the period between July 22 and August 2nd. Velocities of the upwelling fluids were calculated using Diffuse Fluid Velocimetry (DFV; Mittelstaedt et al., 2010). DFV is a cross correlation technique that tracks moving index of refraction anomalies (i.e., hot parcels of fluid) through time. Over the ~12 day deployment, median flow rates ranged from 0.5 cm/s to 6 cm/s and mean fluid temperature anomalies from 0°C up to ~6.5°C, yielding an average heat flux density of 0.23 MW/m2. Spectral analysis of both the measured temperatures and calculated velocities yield a peak in normalized power at the semi-diurnal lunar period (M2, 12.4hrs), but no other spectral peaks above the 95% confidence level. Here, we present these results and discuss their implications for the tidal current and tidal pressure models of diffuse flow variability at the ASHES vent field.

  19. Susceptibility to Heavy Metals and Characterization of Heterotrophic Bacteria Isolated from Two Hydrothermal Vent Polychaete Annelids, Alvinella pompejana and Alvinella caudata

    OpenAIRE

    Jeanthon, Christian; Prieur, Daniel

    1990-01-01

    Specimens of alvinellid polychaetes and their tubes were collected in the Parigo hydrothermal vent field on the East Pacific Rise (13°N) in October and November 1987. Heterotrophic bacterial strains were isolated on metal-amended media from the tube and dorsal integument of one specimen of Alvinella pompejana, from the dorsal integument of another from the whole integument of a specimen of Alvinella caudata, and from undetermined alvinellid tubes. The strains were characterized and tested for...

  20. Bacterial Lifestyle in a Deep-sea Hydrothermal Vent Chimney Revealed by the Genome Sequence of the Thermophilic Bacterium Deferribacter desulfuricans SSM1

    OpenAIRE

    Takaki, Yoshihiro; Shimamura, Shigeru; Nakagawa, Satoshi; Fukuhara, Yasuo; Horikawa, Hiroshi; Ankai, Akiho; Harada, Takeshi; Hosoyama, Akira; Oguchi, Akio; Fukui, Shigehiro; Fujita, Nobuyuki; Takami, Hideto; Takai, Ken

    2010-01-01

    The complete genome sequence of the thermophilic sulphur-reducing bacterium, Deferribacter desulfuricans SMM1, isolated from a hydrothermal vent chimney has been determined. The genome comprises a single circular chromosome of 2 234 389 bp and a megaplasmid of 308 544 bp. Many genes encoded in the genome are most similar to the genes of sulphur- or sulphate-reducing bacterial species within Deltaproteobacteria. The reconstructed central metabolisms showed a heterotrophic lifestyle primarily d...

  1. Rhodopsin in the Dark Hot Sea: Molecular Analysis of Rhodopsin in a Snailfish, Careproctus rhodomelas, Living near the Deep-Sea Hydrothermal Vent.

    Directory of Open Access Journals (Sweden)

    Rie Sakata

    Full Text Available Visual systems in deep-sea fishes have been previously studied from a photobiological aspect; however, those of deep-sea fish inhabiting the hydrothermal vents are far less understood due to sampling difficulties. In this study, we analyzed the visual pigment of a deep-sea snailfish, Careproctus rhodomelas, discovered and collected only near the hydrothermal vents of oceans around Japan. Proteins were solubilized from the C. rhodomelas eyeball and subjected to spectroscopic analysis, which revealed the presence of a pigment characterized by an absorption maximum (λmax at 480 nm. Immunoblot analysis of the ocular protein showed a rhodopsin-like immunoreactivity. We also isolated a retinal cDNA encoding the entire coding sequence of putative C. rhodomelas rhodopsin (CrRh. HEK293EBNA cells were transfected with the CrRh cDNA and the proteins extracted from the cells were subjected to spectroscopic analysis. The recombinant CrRh showed the absorption maximum at 480 nm in the presence of 11-cis retinal. Comparison of the results from the eyeball extract and the recombinant CrRh strongly suggests that CrRh has an A1-based 11-cis-retinal chromophore and works as a photoreceptor in the C. rhodomelas retina, and hence that C. rhodomelas responds to dim blue light much the same as other deep-sea fishes. Because hydrothermal vent is a huge supply of viable food, C. rhodomelas likely do not need to participate diel vertical migration and may recognize the bioluminescence produced by aquatic animals living near the hydrothermal vents.

  2. Thermodynamics of chemical free energy generation in off-axis hydrothermal vent systems and its consequences for compartmentalization and the emergence of life

    CERN Document Server

    Simoncini, E; Gallori, E; .,

    2010-01-01

    In this paper we demonstrate how chemical free energy can be produced by a geological process. We provide a thermodynamic framework in which to assess how life emerged at the off-axis hydrothermal vent system; the RNA - clays system has been investigated from the entropic point of view, showing that the stabilization of the system in a state further away from equilibrium state, by an inorganic heterogeneous compartmetalization phenomena, is able to produce chemical free energy useful for RNA self - replication.

  3. Colonization of plant substrates at hydrothermal vents and cold seeps in the northeast Atlantic and Mediterranean and occurrence of symbiont-related bacteria

    OpenAIRE

    Szafranski, Kamil M.; Deschamps, Philippe; Cunha, Marina R.; Gaudron, Sylvie M.; Duperron, Sébastien

    2015-01-01

    Reducing conditions with elevated sulfide and methane concentrations in ecosystems such as hydrothermal vents, cold seeps or organic falls, are suitable for chemosynthetic primary production. Understanding processes driving bacterial diversity, colonization and dispersal is of prime importance for deep-sea microbial ecology. This study provides a detailed characterization of bacterial assemblages colonizing plant-derived substrates using a standardized approach over a geographic area spanning...

  4. Alkaline hydrothermal de-ashing and desulfurization of low quality coal and its application to hydrogen-rich gas generation

    Energy Technology Data Exchange (ETDEWEB)

    Mursito, Anggoro Tri [Research Centre for Geotechnology, Indonesian Institute of Sciences (LIPI), Jl. Sangkuriang Komplek LIPI, Gd. 70, Bandung 40135 (Indonesia); Department of Earth Resources Engineering, Graduate School of Engineering, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395 (Japan); Hirajima, Tsuyoshi; Sasaki, Keiko [Department of Earth Resources Engineering, Faculty of Engineering, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395 (Japan)

    2011-01-15

    This paper describes experimental research and a fundamental study of alkaline hydrothermal treatment of high-sulfur, high-ash coal from Banten, Java-Indonesia. Experiments were carried out on a laboratory-scale 0.5 L batch reactor. The alkaline hydrothermal treatment gave upgraded clean coal with low sulfur content (about 0.3 wt.%) and low ash content (about 2.1 wt.%). A zero carbon dioxide and pure hydrogen gas were produced at 330 C by introducing an alkali (sodium hydroxide, NaOH) to the hydrothermal treatment of raw coal. X-ray diffraction (XRD) and X-ray fluorescence (XRF) techniques were used to test for the removal or reduction of major inorganic elements in the coal, and changes in carbon-functional groups and their properties were determined by Fourier transform infrared spectroscopy (FTIR) and Carbon-13 of nuclear magnetic resonance ({sup 13}C NMR) tests on the product of the hydrothermal upgrading and demineralization process. (author)

  5. Sources of organic carbon for Rimicaris hybisae: Tracing individual fatty acids at two hydrothermal vent fields in the Mid-Cayman rise

    Science.gov (United States)

    Streit, Kathrin; Bennett, Sarah A.; Van Dover, Cindy L.; Coleman, Max

    2015-06-01

    Hydrothermal vents harbor ecosystems mostly decoupled from organic carbon synthesized with the energy of sunlight (photosynthetic carbon source) but fueled instead by oxidation of reduced compounds to generate a chemosynthetic carbon source. Our study aimed to disentangle photosynthetic and chemosynthetic organic carbon sources for the shrimp species Rimicaris hybisae, a primary consumer presumed to obtain its organic carbon mainly from ectosymbiotic chemoautotrophic bacteria living on its gill cover membrane. To provide ectosymbionts with ideal conditions for chemosynthesis, these shrimp live in dense clusters around vent chimneys; they are, however, also found sparsely distributed adjacent to diffuse vent flows, where they might depend on alternative food sources. Densely and sparsely distributed shrimp were sampled and dissected into abdominal tissue and gill cover membrane, covered with ectosymbiotic bacteria, at two hydrothermal vent fields in the Mid-Cayman rise that differ in vent chemistry. Fatty acids (FA) were extracted from shrimp tissues and their carbon isotopic compositions assessed. The FA data indicate that adult R. hybisae predominantly rely on bacteria for their organic carbon needs. Their FA composition is dominated by common bacterial FA of the n7 family (~41%). Bacterial FA of the n4 FA family are also abundant and found to constitute good biomarkers for gill ectosymbionts. Sparsely distributed shrimp contain fractions of n4 FA in gill cover membranes ~4% lower than densely packed ones (~18%) and much higher fractions of photosynthetic FA in abdominal tissues, ~4% more (compared with 1.6%), suggesting replacement of ectosymbionts along with exoskeletons (molt), while they take up alternative diets of partly photosynthetic organic carbon. Abdominal tissues also contain photosynthetic FA from a second source taken up presumably during an early dispersal phase and still present to c. 3% in adult shrimp. The contribution of photosynthetic carbon to

  6. DIRS1-like retrotransposons are widely distributed among Decapoda and are particularly present in hydrothermal vent organisms

    Directory of Open Access Journals (Sweden)

    Bonnivard Eric

    2009-04-01

    Full Text Available Abstract Background Transposable elements are major constituents of eukaryote genomes and have a great impact on genome structure and stability. Considering their mutational abilities, TEs can contribute to the genetic diversity and evolution of organisms. Knowledge of their distribution among several genomes is an essential condition to study their dynamics and to better understand their role in species evolution. DIRS1-like retrotransposons are a particular group of retrotransposons according to their mode of transposition that implies a tyrosine recombinase. To date, they have been described in a restricted number of species in comparison with the LTR retrotransposons. In this paper, we determine the distribution of DIRS1-like elements among 25 decapod species, 10 of them living in hydrothermal vents that correspond to particularly unstable environments. Results Using PCR approaches, we have identified 15 new DIRS1-like families in 15 diverse decapod species (shrimps, lobsters, crabs and galatheid crabs. Hydrothermal organisms show a particularly great diversity of DIRS1-like elements with 5 families characterized among Alvinocarididae shrimps and 3 in the galatheid crab Munidopsis recta. Phylogenic analyses show that these elements are divergent toward the DIRS1-like families previously described in other crustaceans and arthropods and form a new clade called AlDIRS1. At larger scale, the distribution of DIRS1-like retrotransposons appears more or less patchy depending on the taxa considered. Indeed, a scattered distribution can be observed in the infraorder Brachyura whereas all the species tested in infraorders Caridea and Astacidea harbor some DIRS1-like elements. Conclusion Our results lead to nearly double both the number of DIRS1-like elements described to date, and the number of species known to harbor these ones. In this study, we provide the first degenerate primers designed to look specifically for DIRS1-like retrotransposons. They

  7. Immunomodulatory N-acyl Dopamine Glycosides from the Icelandic Marine Sponge Myxilla incrustans Collected at a Hydrothermal Vent Site.

    Science.gov (United States)

    Einarsdottir, Eydis; Liu, Hong-Bing; Freysdottir, Jona; Gotfredsen, Charlotte Held; Omarsdottir, Sesselja

    2016-06-01

    A chemical investigation of the sponge (Porifera) Myxilla incrustans collected from the unique submarine hydrothermal vent site Strytan, North of Iceland, revealed a novel family of closely related N-acyl dopamine glycosides. Three new compounds, myxillin A (1), B (2) and C (3), were isolated and structurally elucidated using several analytical techniques, such as HR-MS, 1D and 2D NMR spectroscopy. Myxillin A (1) and B (2)were shown to be structurally similar, composed of a dopamine moiety, but differ in the acyl chain length and saturation. The myxillin C (3) has a dehydrotyrosine moiety composing the same acyl chain and glycosylation as myxillin B (2). Myxillins A (1) and C (3) were tested for immunomodulating activity in an in vitro dendritic cell model. Dendritic cells matured and stimulated in the presence of myxillin A (1) secreted lower levels of IL-12p40, whilst dendritic cells matured and stimulated in the presence of myxillin C (3) secreted lower levels of IL-10 compared with dendritic cells matured and stimulated in the presence of the solvent alone. These opposing results indicate that the structural differences in the aromatic ring part of the molecules could have an impact on the immunological effects of dendritic cells. These molecules could, therefore, prove to be important in preventing inflammatory diseases on the one hand, and inducing a response to fight tumors and/or pathogens on the other hand. Further studies will be needed to confirm these potential uses. PMID:27135626

  8. Hemoglobins from deep-sea hydrothermal vent scaleworms of the genus Branchipolynoe: a new type of quaternary structure.

    Science.gov (United States)

    Hourdez, S; Lallier, F H; Green, B N; Toulmond, A

    1999-03-01

    Branchipolynoe symmytilida and B. seepensis are two scaleworms (Polychaeta; Polynoidae) living commensally in the mantle cavity of deep-sea hydrothermal vent and cold-seep mussels. In contrast with littoral members of this family, the two species exhibit a large amount of extracellular hemoglobin (Hb) in their coelomic fluid. Gel filtration revealed the existence of four different Hbs: one minor, high molecular mass (3x10(6) Da) Hb, V1-Hb, reminiscent of a vascular hexagonal bilayer annelid Hb; two major coelomic Hbs, C1-Hb, and C2-Hb, with unusual masses for extracellular annelid Hbs of 153 and 124 kDa respectively; and a minor probably coelomic Hb of 23 kDa (C3-Hb). Using electrospray ionization mass spectrometry, SDS-PAGE after subtilisin treatment, and tandem mass spectrometry, we showed that C1-Hb is a trimer of a 57,996 Da chain and C2-Hb is a dimer of a 57,648 Da chain, each chain being a four-domain/four-heme polypeptide. This multimeric, multidomain arrangement is unique among annelid Hbs and appears different from that of other known multidomain Hbs. PMID:10081955

  9. Three-dimensional reconstruction of the hexagonal bilayer hemoglobin of the hydrothermal vent tube worm Riftia pachyptila by cryoelectron microscopy.

    Science.gov (United States)

    de Haas, F; Zal, F; Lallier, F H; Toulmond, A; Lamy, J N

    1996-11-01

    A frozen-hydrated specimen of the V1 hemoglobin of the hydrothermal vent tube worm Riftia pachyptila was observed in the electron microscope and subjected to three-dimensional reconstruction by the method of random conical tilt series. The 3D volume possesses a D6 point-group symmetry. When viewed along its 6-fold axis the vertices of its upper hexagonal layer are 16 degrees clockwise rotated compared to those of the lower layer. A central linker complex is decorated by 12 hollow globular substructures. The linker complex comprises (i) a central hexagonal toroid, (ii) two internal bracelets onto which the hollow globular substructures are built, and (iii) six structures connecting the two hexagonal layers. The hollow globular substructures, related to the dodecamers of globin chains resulting from the dissociation of the hexagonal bilayer hemoglobin, have a local pseudo 3-fold symmetry and are composed each of three elongated structures visible when the volume is displayed at high threshold. At a resolution of 36 A, the 3D volumes of the hexagonal bilayer hemoglobins of Riftia pachyptyla and of the leech Macrobdella decora look almost perfectly identical. PMID:8953646

  10. Study of microorganisms/basaltic crust interactions at hydrothermal vents and abyssal environments by an in situ experimental approach

    Science.gov (United States)

    Henri, P. A.; Rommevaux, C.; Menez, B.; Lesongeur, F.; Godfroy, A.

    2013-12-01

    In the dark ocean, microbial communities must thrive on organic matter coming from the photic zone or on redox reaction through chemosynthesis. Chemosynthetic microorganisms need to couple oxidized compounds, which are abundant in seawater, to reduced compounds. Near ocean ridges, newly formed basalt is enriched in these compounds. Even if the bioavailability is weak in glassy basalts, organisms can potentially alter the rock directly or indirectly by the use of these compounds (e.g. Fe2+, Mn2+) for their metabolisms. Moreover, the oceanic crust is highly hydrated by the oceanic circulation, and abiotic alteration, by hydration and redox reactions, contribute to make compounds bioavailable for organisms. To better discern abiotic and biotic implications and contribution in the early basalt alteration, we conducted an in situ experiment. Microbial incubators and abiotic controls containing synthetic MORB glasses of various compositions have been deployed since 2006 off-axis and in the vicinity of various vents of the Lucky Strike hydrothermal field (LS; 37°N-32°W, MAR) and recovered annually. This allows us to compare the biodiversity and the associated level of basalt alteration, according to the duration of in situ incubation (from only few weeks to more than two years) and the environmental parameters (particularly the level of hydrothermal fluid influence). The nature and the level of the microbial colonization of each sample are explored by pyrosequencing targeting the 16S RNAr gene. In parallel, the basalt alteration is characterized at the appropriate microscale using electronic, fluorescence and Raman microscopy/spectroscopy. These approaches allow to identify microbial species specifically associated with the alteration phases and to link alteration processes and microbial metabolisms and activity. We first evidence a close association between the different alteration phases at the basalt surface, and the presence of microbial cells, in favor of a

  11. Characterization and functional properties of the extracellular coelomic hemoglobins from the deep-sea, hydrothermal vent scaleworm Branchipolynoe symmytilida.

    Science.gov (United States)

    Hourdez, S; Lallier, F H; Martin-Jézéquel, V; Weber, R E; Toulmond, A

    1999-03-01

    Polychaete species belonging to the genus Branchipolynoe are commensal with mussels from deep-sea hydrothermal vents and cold-seeps. Possessing hemoglobins (Hbs), the species B. symmytilida, which is found in the mussel Bathymodiolus thermophilus on the East Pacific Rise, is exceptional in a family normally devoid of respiratory pigments. In a previous paper we described two major coelomic extracellular hemoglobins with unique quaternary structures. Aiming to discern respiratory adaptations to the highly variable hydrothermal environment, this paper characterizes the functional properties of these Hbs and the coelomic fluid. The two major hemoglobins (C1 and C2) exhibit spectrophotometric characteristics of both intra- and extracellular hemoglobins. However, their amino acid content is very different from other known hemoglobins and is characterized by a high proportion of alanine and glycine (up to 40% cumulated in C1). C1 and C2 differ markedly by their cysteine content (0.8% and 13% respectively). The coelomic fluid exhibits a strong buffer capacity due to the high hemoglobin content (3 mM heme). In vitro, CO2 accumulation (up to 10-12 mM CO2 for PCO2 = 7.5 Torr) occurs with limited pH changes and is only partly accounted for by carbamino-Hb formation. The two hemoglobins exhibit high oxygen-affinities (P50 0.4 Torr for C1 and 0.9 Torr for C2, at 10 degrees C, pH 8) and a normal Bohr effect (phi values ranging from -0.54 and -0.37 at 10 degrees C, to -0.24 and -0.28 at 30 degrees C, for C1 and C2, respectively). Cooperativity values range from 0.8 to 1.9 for C1 and from 0.8 to 1.7 for C2. The temperature sensitivity of O2 affinity reflect deltaH values that decrease from -30 to -60 kJ x mol(-1) with increasing pH. C2 exhibits a slight specific effect of CO2 on oxygenation properties. PMID:10081956

  12. Thermococcus thioreducens sp. nov., a Novel Hyperthermophilic, Obligately Sulfur-Reducing Archaeon from a Deep-Sea Hydrothermal Vent

    Science.gov (United States)

    Pikuta, Elena V.; Marsic, Damien; Itoh, Takashi; Bej, Asim K.; Tang, Jane; Whitman, William B.; Ng, Joseph D.; Garriott, Owen K.; Hoover, Richard B.

    2007-01-01

    A hyperthermophilic, sulfur-reducing, organo-heterotrophic archaeon, strain OGL-20P(sup T), was isolated from 'black smoker' chimney material from the Rainbow hydrothermal vent site on the Mid-Atlantic Ridge (36.2degN, 33.9degW). The cells of strain OGL-20P(T) have an irregular coccoid shape and are motile with a single flagellum. Growth was observed within a pH range of 5.0-8.5 (optimum pH 7.0), an NaCl concentration range of 1-5%(w/v) (optimum 3%)and a temperature range of 55-94 C (optimum 83-85 C). The novel isolate is strictly anaerobic and obligately dependent upon elemental sulfur as an electron acceptor, but it does not reduce sulfate, sulfite, thiosulfate, Fe(III) or nitrate. Proteolysis products (peptone, bacto-tryptone, Casamino acids and yeast extract) are utilized as substrates during sulfur reduction. Strain OGL-20P(sup T) is resistant to ampicillin, chloram phenicol, kanamycin and gentamicin, but sensitive to tetracycline and rifampicin. The G + C content of the DNA is 52.9 mol% The 16S rRNA gene sequence analysis revealed that strain OGL-20P(sup T) is closely related to Thermococcus coalescens and related species, but no significant homology by DNA-DNA hybridization was observed between those species and the new isolate. On the basis of physiological and molecular properties of the new isolate, we conclude that strain OGL-20P(sup T) represents a new separate species within the genus Thermococcus, for which we propose the name Thermococcus thioreducens sp. nov. The type strain is OGL-20P(sup T) (=JCM 12859(exp T) = DSM 14981(exp T)=ATCC BAA-394(exp T)).

  13. Hypotaurine, N-methyltaurine, taurine, and glycine betaine as dominant osmolytes of vestimentiferan tubeworms from hydrothermal vents and cold seeps.

    Science.gov (United States)

    Yin, M; Palmer, H R; Fyfe-Johnson, A L; Bedford, J J; Smith, R A; Yancey, P H

    2000-01-01

    Organic osmolytes, solutes that regulate cell volume, occur at high levels in marine invertebrates. These are mostly free amino acids such as taurine, which are "compatible" with cell macromolecules, and methylamines such as trimethylamine oxide, which may have a nonosmotic role as a protein stabilizer, and which is higher in many deep-sea animals. To better understand nonosmotic roles of osmolytes, we used high-performance liquid chromatography and (1)H-nuclear magnetic resonance (NMR) to analyze vestimentiferans (vestimentum tissue) from unusual marine habitats. Species from deep hydrothermal vents were Riftia pachyptila of the East Pacific Rise (2,636 m) and Ridgeia piscesae of the Juan de Fuca Ridge (2,200 m). Species from cold hydrocarbon seeps were Lamellibrachia sp. and an unnamed escarpid species from subtidal sediment seeps (540 m) off Louisiana and Lamellibrachia barhami from bathyal tectonic seeps (1,800-2,000 m) off Oregon. Riftia were dominated by hypotaurine (152 mmol/kg wet wt), an antioxidant, and an unidentified solute with an NMR spectrum consistent with a methylamine. Ridgeia were dominated by betaine (N-trimethylglycine; 109 mmol/kg), hypotaurine (64 mmol/kg), and taurine (61 mmol/kg). The escarpids were dominated by taurine (138 mmol/kg) and hypotaurine (69 mmol/kg). Both Lamellibrachia populations were dominated by N-methyltaurine (209-252 mmol/kg), not previously reported as a major osmolyte, which may be involved in methane and sulfate metabolism. Trunk and plume tissue of the Oregon Lamellibrachia were nearly identical to vestimentum in osmolyte composition. The methylamines may also stabilize proteins against pressure; they were significantly higher in the three deeper-dwelling groups. PMID:11073799

  14. Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent Mytilid fatty acids by 13C-fingerprinting

    Directory of Open Access Journals (Sweden)

    F. Dehairs

    2010-05-01

    Full Text Available Bathymodiolus azoricus mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part of their energy requirements are met via an endosymbiotic association with chemolithotrophic and methanotrophic bacteria. In an effort to describe phenotypic characteristics of the two bacterial endosymbionts and to assess their ability to assimilate CO2, CH4 and multi-carbon compounds, we performed experiments in aquaria using 13C-labeled NaHCO3 (in the presence of H2S, CH4 or amino-acids and traced the incorporation of 13C into total and phospholipid fatty acids (tFA and PLFA, respectively. 14:0, 15:0, 16:1(n-7c+t and 18:1(n-7c+t PLFA were labeled in the presence of H13CO3- (+H2S and 13CH4, while the 12:0 compound became labeled only in the presence of H13CO3− (+H2S. In contrast, the 16:1(n-9, 16:1(n-8 and (n-6, 18:1(n-8c and (n-7, 20:1(n-7 and 18:2(n-7 PLFA were only labeled in the presence of 13CH4. Some of these symbiont-specific fatty acids also appeared to be labeled in mussel gill tFA when incubated with 13C-enriched amino acids, and so were mussel-specific fatty acids such as 22:2(n-7,15. Our results provide experimental evidence for the potential of specific fatty acid markers to distinguish between the two endosymbiotic bacteria, shedding new light on C1 and multi-carbon compound metabolic pathways in B. azoricus and its symbionts.

  15. In-Situ pH Measurements in Mid-Ocean Ridge Hydrothermal Vent Fluids: Constraints on Subseafloor Alteration Processes at Crustal Depths

    Science.gov (United States)

    Schaen, A. T.; Ding, K.; Seyfried, W. E.

    2013-12-01

    Developments in electrochemistry and material science have facilitated the construction of ceramic (YSZ) based chemical sensor systems that can be used to measure and monitor pH and redox in aqueous fluids at elevated temperatures and pressures. In recent years, these sensor systems have been deployed to acquire real-time and time series in-situ data for high-temperature hydrothermal vent fluids at the Main Endeavour Field (Juan de Fuca Ridge), 9oN (East Pacific Rise), and at the ultramafic-hosted Rainbow field (36oN, Mid-Atlantic Ridge). Here we review in-situ pH data measured at these sites and apply these data to estimate the pH of fluids ascending to the seafloor from hydrothermal alteration zones deeper in the crust. In general, in-situ pH measured at virtually all vent sites is well in excess of that measured shipboard owing to the effects of temperature on the distribution of aqueous species and the solubility of metal sulfides, especially Cu and Zn, originally dissolved in the vent fluids. In situ pH measurements determined at MEF (Sully vent) and EPR 9oN (P-vent) in 2005 and 2008 were 4.4 ×0.02 and 5.05×0.05, respectively. The temperature and pressure (seafloor) of the vent fluids at each of the respective sites were 356oC and 220 bar, and 380oC and 250 bar. Plotting these data with respect to fluid density reveals that the in-situ pH of each vent fluid is approximately 1.5 pH units below neutrality. The density-pH (in-situ) correlation, however, is important because it provides a means from which the vent fluids were derived. Using dissolved silica and chloride from fluid samples at the MEF (Sully) suggest T/P conditions of approximately 435oC, 380 bar, based on quartz-fluid and NaCl-H2O systems. At the fluid density calculated for these conditions, pH (in-situ) is predicted to be ~6.2. Attempts are presently underway to assess the effect of the calculated pH on metal sulfide and silicate (e.g., plagioclase, chlorite) solubility in comparison with

  16. Zn-Driven Discovery of a Hydrothermal Vent Fungal Metabolite Clavatustide C, and an Experimental Study of the Anti-Cancer Mechanism of Clavatustide B

    Directory of Open Access Journals (Sweden)

    Panpan Ye

    2014-05-01

    Full Text Available A naturally new cyclopeptide, clavatustide C, was produced as a stress metabolite in response to abiotic stress elicitation by one of the hydrothermal vent fluid components Zn in the cultured mycelia of Aspergillus clavatus C2WU, which were isolated from Xenograpsus testudinatus. X. testudinatus lives at extreme, toxic habitat around the sulphur-rich hydrothermal vents in Taiwan Kueishantao. The known compound clavatustide B was also isolated and purified. This is the first example of a new hydrothermal vent microbial secondary metabolite produced in response to abiotic Zn treatment. The structures were established by spectroscopic means. The regulation of G1-S transition in hepatocellular carcinoma cell lines by clavatustide B was observed in our previous study. The purpose of the present study was to verify these results in other types of cancer cell lines and elucidate the possible molecular mechanism for the anti-cancer activities of clavatustide B. In different human cancer cell lines, including pancreatic cancer (Panc-1, gastric cancer (MGC-803, colorectal cancer (SW-480, retinoblastoma (WERI-Rb-1 and prostate cancer (PC3, clavatustide B efficiently suppressed cell proliferations in a dose-dependent manner. Although different cancer cell lines presented variety in Max effect dose and IC50 dose, all cancer cell lines showed a lower Max effect dose and IC50 dose compared with human fibroblasts (hFB (p < 0.05. Moreover, significant accumulations in G1 phases and a reduction in S phases (p < 0.05 were observed under clavatustide B treatment. The expression levels of 2622 genes including 39 cell cycle-associated genes in HepG2 cells were significantly altered by the treatment with 15 μg/mL clavatustide B after 48 h. CCNE2 (cyclin E2 was proved to be the key regulator of clavatustide B-induced G1-S transition blocking in several cancer cell lines by using real-time PCR.

  17. High-throughput sequencing and analysis of the gill tissue transcriptome from the deep-sea hydrothermal vent mussel Bathymodiolus azoricus

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    Gomes Paula

    2010-10-01

    Full Text Available Abstract Background Bathymodiolus azoricus is a deep-sea hydrothermal vent mussel found in association with large faunal communities living in chemosynthetic environments at the bottom of the sea floor near the Azores Islands. Investigation of the exceptional physiological reactions that vent mussels have adopted in their habitat, including responses to environmental microbes, remains a difficult challenge for deep-sea biologists. In an attempt to reveal genes potentially involved in the deep-sea mussel innate immunity we carried out a high-throughput sequence analysis of freshly collected B. azoricus transcriptome using gills tissues as the primary source of immune transcripts given its strategic role in filtering the surrounding waterborne potentially infectious microorganisms. Additionally, a substantial EST data set was produced and from which a comprehensive collection of genes coding for putative proteins was organized in a dedicated database, "DeepSeaVent" the first deep-sea vent animal transcriptome database based on the 454 pyrosequencing technology. Results A normalized cDNA library from gills tissue was sequenced in a full 454 GS-FLX run, producing 778,996 sequencing reads. Assembly of the high quality reads resulted in 75,407 contigs of which 3,071 were singletons. A total of 39,425 transcripts were conceptually translated into amino-sequences of which 22,023 matched known proteins in the NCBI non-redundant protein database, 15,839 revealed conserved protein domains through InterPro functional classification and 9,584 were assigned with Gene Ontology terms. Queries conducted within the database enabled the identification of genes putatively involved in immune and inflammatory reactions which had not been previously evidenced in the vent mussel. Their physical counterpart was confirmed by semi-quantitative quantitative Reverse-Transcription-Polymerase Chain Reactions (RT-PCR and their RNA transcription level by quantitative PCR (q

  18. Lipid and lipid carbon stable isotope composition of the hydrothermal vent shrimp Mirocaris fortunata: evidence for nutritional dependence on photosynthetically fixed carbon

    OpenAIRE

    Pond, David W; Segonzac, Michel; Bell, Michael V; Dixon, David R.; Fallick, Anthony E.; Sargent, John R.

    1997-01-01

    Mirocaris fortunata were sampled from the Lucky Strike hydrothermal vent area (Eiffel Tower site) on the mid-Atlantic ridge during the French DIVA 2 cruise (June 1994). Small adults (17 to 22 mm total length), although morphologically identical, could be divided into 2 categories on the basis of pigmentation, lipid composition and C-13/C-12 stable isotope ratios of fatty acids. Highly pigmented small adults (8.6 to 9.2 mu g carotenoid shrimp(-1)) contained higher levels of total lipid than si...

  19. Abundant Hydrothermal Venting in the Southern Ocean Near 62°S/159°E on the Australian-Antarctic Ridge

    Science.gov (United States)

    Baker, E. T.; Hahm, D.; Rhee, T. S.; Park, S. H.; Lupton, J. E.; Walker, S. L.; Choi, H.

    2014-12-01

    Circum-Antarctic Ridges (CARs) comprise almost one-third of the global Mid-Ocean Ridge, yet remain terra incognita for hydrothermal activity and chemosynthetic ecosystems. The InterRidge Vents Database lists only 3 confirmed (visualized) and 35 inferred (plume evidence) active sites along the ~21,000 km of CARs. Here, we report on a multi-year effort to locate and characterize hydrothermal activity on two 1st-order segments of the Australian-Antarctic Ridge that are perhaps more isolated from other known vent fields than any other vent site on the Mid-Ocean Ridge. KR1 is a 300-km-long segment near 62°S/159°E, and KR2 a 90-km-long segment near 60°S/152.5°E. We used profiles collected by Miniature Autonomous Plume Recorders (MAPRs) on rock corers in March and December of 2011 to survey each segment, and an intensive CTD survey in Jan/Feb 2013 to pinpoint sites and sample plumes on KR1. Optical and oxidation-reduction potential (ORP, aka Eh) anomalies indicate multiple active sites on both segments. Seven profiles on KR2 found 3 sites, each separated by ~25 km. Forty profiles on KR1 identified 13 sites, some within a few km of each other. The densest site concentration on KR1 occurred along a relatively inflated, 90-km-long section near the segment center. CTD tows covered 20 km of the eastern, most inflated portion of this area, finding two 6-km-long zones centered near 158.6°E and 158.8°E with multiple plume anomalies. Three ORP anomalies within 50 m of the seafloor indicate precise venting locations. We call this area the Mujin "Misty Harbor" vent field. Vent frequency sharply decreases away from Mujin. 3He/heat ratios determined from 20 plume samples in the Mujin field were mostly spreading ridges. We calculate the plume incidence (ph) along KR1 and KR2 as the mean of the fraction of MAPR casts detecting a plume in each 2nd-order segment. For all 6 segments, ph=0.37±0.25, consistent with the prediction of 0.33 from the global trend of ph for a spreading

  20. Temporal variation in the antioxidant defence system and lipid peroxidation in the gills and mantle of hydrothermal vent mussel Bathymodiolus azoricus

    Science.gov (United States)

    Company, Rui; Serafim, Angela; Cosson, Richard; Fiala-Médioni, Aline; Dixon, David; João Bebianno, Maria

    2006-07-01

    Hydrothermal vent mussels are exposed continually to toxic compounds, including high metal concentrations and other substances like dissolved sulphide, methane and natural radioactivity. Fluctuations in these parameters appear to be common because of the characteristic instability of the hydrothermal environment. Temporal variation in the antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), total glutathione peroxidases (Total GPx), selenium dependent glutathione peroxidases (Se-GPx)), metallothioneins and lipid peroxidation (LPO) in the gills and mantle of the mussel Bathymodiolus azoricus from Menez-Gwen hydrothermal vent site was evaluated and related to the accumulated metal concentrations (Ag, Cu, Cd, Fe, Mn and Zn) in the tissues. Maximum antioxidant enzyme activities in the gills were detected in the beginning of summer, followed by a gradual decrease throughout the following months. One year after, the levels of antioxidant enzyme activities were similar to those reported one year before. LPO in this tissue exhibited a similar temporal variation trend. A different pattern of temporal variation in antioxidant enzyme activities was observed in the mantle, with a gradual increase from summer to the end of autumn (November). LPO in the mantle exhibited an almost reverse trend of temporal variation to that of antioxidant enzyme activities in this tissue. Antioxidant defences in the gills of B. azoricus were significantly enhanced with increasing concentrations of Ag, Cu and Mn, while negative relationships between antioxidant enzymes and Cd, Cu, Mn and Zn concentrations in the mantle were observed, suggesting different pathways of reactive oxygen species (ROS) production and that these tissues responded differently to the metal accumulation. However, temporal variation in biomarkers of defence and damage were in general similar to coastal bivalve species and can be associated with temporal variations of the physiological status due to reproduction

  1. Tracing carbon assimilation in endosymbiotic deep-sea hydrothermal vent Mytilid fatty acids by 13C-fingerprinting

    Directory of Open Access Journals (Sweden)

    F. Dehairs

    2010-09-01

    Full Text Available Bathymodiolus azoricus mussels thrive at Mid-Atlantic Ridge hydrothermal vents, where part of their energy requirements are met via an endosymbiotic association with chemolithotrophic and methanotrophic bacteria. In an effort to describe phenotypic characteristics of the two bacterial endosymbionts and to assess their ability to assimilate CO2, CH4 and multi-carbon compounds, we performed experiments in aquaria using 13C-labeled NaHCO3 (in the presence of H2S, CH4 or amino-acids and traced the incorporation of 13C into total and phospholipid fatty acids (tFA and PLFA, respectively. 14:0; 15:0; 16:0; 16:1(n − 7c+t; 18:1(n − 13c+t and (n − 7c+t; 20:1(n − 7; 20:2(n − 9,15; 18:3(n − 7 and (n − 5,10,13 PLFA were labeled in the presence of H13CO3− (+H2S and 13CH4, while the 12:0 compound became labeled only in the presence of H13CO3− (+H2S. In contrast, the 17:0; 18:0; 16:1(n − 9; 16:1(n − 8 and (n − 6; 18:1(n − 8; and 18:2(n − 7 PLFA were only labeled in the presence of 13CH4. Some of these symbiont-specific fatty acids also appeared to be labeled in mussel gill tFA when incubated with 13C-enriched amino acids, and so were mussel-specific fatty acids such as 22:2(n − 7,15. Our results provide experimental evidence for the potential of specific fatty acid markers to distinguish between the two endosymbiotic bacteria, shedding new light on C1 and multi-carbon compound metabolic pathways in B. azoricus and its symbionts.

  2. Cetia pacifica gen. nov., sp. nov., a chemolithoautotrophic, thermophilic, nitrate-ammonifying bacterium from a deep-sea hydrothermal vent.

    Science.gov (United States)

    Grosche, Ashley; Sekaran, Hema; Pérez-Rodríguez, Ileana; Starovoytov, Valentin; Vetriani, Costantino

    2015-04-01

    A thermophilic, anaerobic, chemolithoautotrophic bacterium, strain TB-6(T), was isolated from a deep-sea hydrothermal vent located on the East Pacific Rise at 9° N. The cells were Gram-staining-negative and rod-shaped with one or more polar flagella. Cell size was approximately 1-1.5 µm in length and 0.5 µm in width. Strain TB-6(T) grew between 45 and 70 °C (optimum 55-60 °C), 0 and 35 g NaCl l(-1) (optimum 20-30 g l(-1)) and pH 4.5 and 7.5 (optimum pH 5.5-6.0). Generation time under optimal conditions was 2 h. Growth of strain TB-6(T) occurred with H2 as the energy source, CO2 as the carbon source and nitrate or sulfur as electron acceptors, with formation of ammonium or hydrogen sulfide, respectively. Acetate, (+)-d-glucose, Casamino acids, sucrose and yeast extract were not used as carbon and energy sources. Inhibition of growth occurred in the presence of lactate, peptone and tryptone under a H2/CO2 (80 : 20; 200 kPa) gas phase. Thiosulfate, sulfite, arsenate, selenate and oxygen were not used as electron acceptors. The G+C content of the genomic DNA was 36.8 mol%. Phylogenetic analysis of the 16S rRNA gene of strain TB-6(T) showed that this organism branched separately from the three most closely related genera, Caminibacter , Nautilia and Lebetimonas , within the family Nautiliaceae . Strain TB-6(T) contained several unique fatty acids in comparison with other members of the family Nautiliaceae . Based on experimental evidence, it is proposed that the organism represents a novel species and genus within the family Nautiliaceae , Cetia pacifica, gen. nov., sp. nov. The type strain is TB-6(T) ( = DSM 27783(T) = JCM 19563(T)). PMID:25604337

  3. Differential gene expression in the mussel Bathymodiolus azoricus from the Menez Gwen and Lucky Strike deep-sea hydrothermal vent sites

    Directory of Open Access Journals (Sweden)

    M. Pinheiro

    2013-02-01

    Full Text Available The deep-sea hydrothermal vent mussel Bathymodiolus azoricus is a symbiont bearing bivalve that is found in great abundance at the Menez Gwen and Lucky Strike vent sites and in close vicinity off the Azores region near the Mid-Atlantic Ridge (MAR. The distinct relationships that vent mussels have developed with their physical and chemical environments are likely reflected in global gene expression profiles providing thus a means to distinguish geographically distinct vent mussels on the basis of gene expression studies, fluorescence in situ hybridization (FISH experiments and 16S rRNA amplicon sequencing, to assess the natural expression of bacterial genes and vent mussel immune genes and the constitutive distribution and relative abundance of endosymbiotic bacteria within gill tissues. Our results confirmed the presence of methanotroph-related endosymbionts in Menez Gwen vent mussels whereas Lucky Strike specimens seem to harbor a different bacterial morphotype when a methane monooxygenase gene specific probe was used. No qualitative differences could be visualized between Menez Gwen and Lucky Strike individuals when tested with sulfur-oxidizing-related nucleic-acid probe. Quantitative PCR (qPCR studies revealed varied gene expression profiles in both Menez Gwen and Lucky Strike mussel gill tissues for the immune genes selected. Genes encoding transcription factors presented noticeably low levels of fold expression whether in MG or LS animals whereas the genes encoding effector molecules appeared to have higher levels expression in MG gill tissues. The peptidoglycan recognition molecule, encoding gene, PGRP presented the highest level of transcriptional activity among the genes analyzed in MG gill tissues, seconded by carcinolectin and thus denoting the relevance of immune recognition molecules in early stage of the immune responses onset. Genes regarded as encoding molecules involved in signaling pathways were consistently expressed in both MG

  4. Fluid flow rate, temperature and heat flux at Mohns Ridge vent fields: evidence from isosampler measurements for phase separated hydrothermal circulation along the arctic ridge system

    Science.gov (United States)

    Schultz, A.; Pedersen, R. B.; Thorseth, I. H.; Taylor, P.; Flynn, M.

    2005-12-01

    An expedition to the Mohns Ridge in the Norwegian-Greenland sea was carried out in July-August 2005 as part of BIODEEP, lead by University of Bergen (UoB). UoB had previously detected water column methane along this very slow spreading ridge. Previous ROV observations along the ridge (71 deg 18'N, 5 deg 47'W, 605 mbsl) near Jan Mayen had uncovered a broad area of ferric hydroxide-rich bacterial/mineral assemblages, comprising large populations of gallionella bacteria. This area was revisted in 2005. Characteristic of sections of this area ("Gallionella Garden") are chimney-like structures standing ~15 cm tall, often topped by a sea lily (heliometra glacialis). The interior of the structures comprised quasi-concentric bands with vertically-oriented channels. The Oregon State University/Cardiff University Isosampler sensor determined that some of these assemblages support fluid flow through their interior. The outflow from the chimney structures was typically +0.5 deg C, against background temperatures of -0.3 deg C. Flow anomalies were also identified atop extensive bacterial mats. Gallionella Gardens is several km in extent with active, albeit extremely low temperature hydrothermal flow. A field of active high temperature smoker chimney structures was located near Gallionella Garden at 540 mbsl. This field extends ~500 m along a scarp wall, with hydrothermal mounds extending along faults running perpendicular to the scarp, each of which has multiple smoker vents and areas of diffuse flow. There was evidence for phase separation, with a negatively buoyant fluid phase exiting some vent orifices and descending along the vent wall; and evidence for gas phase condensing after leaving some vent orifices. Gas bubble emissions were not uncommon. Isosampler sensors were available that were configured for lower temperature measurements at Gallionella Garden. While capable of detecting variations in effluent at the 4 millidegree level, the temperature ceiling for the sensor

  5. Isolation and complete genome sequence of the thermophilic Geobacillus sp. 12AMOR1 from an Arctic deep-sea hydrothermal vent site.

    Science.gov (United States)

    Wissuwa, Juliane; Stokke, Runar; Fedøy, Anita-Elin; Lian, Kjersti; Smalås, Arne Oskar; Steen, Ida Helene

    2016-01-01

    Members of the genus Geobacillus have been isolated from a wide variety of habitats worldwide and are the subject for targeted enzyme utilization in various industrial applications. Here we report the isolation and complete genome sequence of the thermophilic starch-degrading Geobacillus sp. 12AMOR1. The strain 12AMOR1 was isolated from deep-sea hot sediment at the Jan Mayen hydrothermal Vent Site. Geobacillus sp. 12AMOR1 consists of a 3,410,035 bp circular chromosome and a 32,689 bp plasmid with a G + C content of 52 % and 47 %, respectively. The genome comprises 3323 protein-coding genes, 88 tRNA species and 10 rRNA operons. The isolate grows on a suite of sugars, complex polysaccharides and proteinous carbon sources. Accordingly, a versatility of genes encoding carbohydrate-active enzymes (CAZy) and peptidases were identified in the genome. Expression, purification and characterization of an enzyme of the glycoside hydrolase family 13 revealed a starch-degrading capacity and high thermal stability with a melting temperature of 76.4 °C. Altogether, the data obtained point to a new isolate from a marine hydrothermal vent with a large bioprospecting potential. PMID:26913091

  6. Bacterial lifestyle in a deep-sea hydrothermal vent chimney revealed by the genome sequence of the thermophilic bacterium Deferribacter desulfuricans SSM1.

    Science.gov (United States)

    Takaki, Yoshihiro; Shimamura, Shigeru; Nakagawa, Satoshi; Fukuhara, Yasuo; Horikawa, Hiroshi; Ankai, Akiho; Harada, Takeshi; Hosoyama, Akira; Oguchi, Akio; Fukui, Shigehiro; Fujita, Nobuyuki; Takami, Hideto; Takai, Ken

    2010-06-01

    The complete genome sequence of the thermophilic sulphur-reducing bacterium, Deferribacter desulfuricans SMM1, isolated from a hydrothermal vent chimney has been determined. The genome comprises a single circular chromosome of 2,234,389 bp and a megaplasmid of 308,544 bp. Many genes encoded in the genome are most similar to the genes of sulphur- or sulphate-reducing bacterial species within Deltaproteobacteria. The reconstructed central metabolisms showed a heterotrophic lifestyle primarily driven by C1 to C3 organics, e.g. formate, acetate, and pyruvate, and also suggested that the inability of autotrophy via a reductive tricarboxylic acid cycle may be due to the lack of ATP-dependent citrate lyase. In addition, the genome encodes numerous genes for chemoreceptors, chemotaxis-like systems, and signal transduction machineries. These signalling networks may be linked to this bacterium's versatile energy metabolisms and may provide ecophysiological advantages for D. desulfuricans SSM1 thriving in the physically and chemically fluctuating environments near hydrothermal vents. This is the first genome sequence from the phylum Deferribacteres. PMID:20189949

  7. The Production of Methane, Hydrogen, and Organic Compounds in Ultramafic-Hosted Hydrothermal Vents of the Mid-Atlantic Ridge

    OpenAIRE

    Konn, C.; Charlou, J.L.; Holm, N.G.; Mousis, O.

    2015-01-01

    Abstract Both hydrogen and methane are consistently discharged in large quantities in hydrothermal fluids issued from ultramafic-hosted hydrothermal fields discovered along the Mid-Atlantic Ridge. Considering the vast number of these fields discovered or inferred, hydrothermal fluxes represent a significant input of H2 and CH4 to the ocean. Although there are lines of evidence of their abiogenic formation from stable C and H isotope results, laboratory experiments, and thermodynamic data, nei...

  8. The Production of Methane, Hydrogen, and Organic Compounds in Ultramafic-Hosted Hydrothermal Vents of the Mid-Atlantic Ridge

    OpenAIRE

    Konn, Cecile; Charlou, Jean-Luc; Holm, N.G.; Mousis, O.

    2015-01-01

    Both hydrogen and methane are consistently discharged in large quantities in hydrothermal fluids issued from ultramafic-hosted hydrothermal fields discovered along the Mid-Atlantic Ridge. Considering the vast number of these fields discovered or inferred, hydrothermal fluxes represent a significant input of H-2 and CH4 to the ocean. Although there are lines of evidence of their abiogenic formation from stable C and H isotope results, laboratory experiments, and thermodynamic data, neither the...

  9. Diffuse venting at the ASHES hydrothermal field: Heat flux and tidally modulated flow variability derived from in situ time-series measurements

    Science.gov (United States)

    Mittelstaedt, Eric; Fornari, Daniel J.; Crone, Timothy J.; Kinsey, James; Kelley, Deborah; Elend, Mitch

    2016-04-01

    Time-series measurements of diffuse exit-fluid temperature and velocity collected with a new, deep-sea camera, and temperature measurement system, the Diffuse Effluent Measurement System (DEMS), were examined from a fracture network within the ASHES hydrothermal field located in the caldera of Axial Seamount, Juan de Fuca Ridge. The DEMS was installed using the HOV Alvin above a fracture near the Phoenix vent. The system collected 20 s of 20 Hz video imagery and 24 s of 1 Hz temperature measurements each hour between 22 July and 2 August 2014. Fluid velocities were calculated using the Diffuse Fluid Velocimetry (DFV) technique. Over the ˜12 day deployment, median upwelling rates and mean fluid temperature anomalies ranged from 0.5 to 6 cm/s and 0°C to ˜6.5°C above ambient, yielding a heat flux of 0.29 ± 0.22 MW m-2 and heat output of 3.1± 2.5 kW. Using a photo mosaic to measure fracture dimensions, the total diffuse heat output from cracks across ASHES field is estimated to be 2.05 ± 1.95 MW. Variability in temperatures and velocities are strongest at semidiurnal periods and show significant coherence with tidal height variations. These data indicate that periodic variability near Phoenix vent is modulated both by tidally controlled bottom currents and seafloor pressure, with seafloor pressures being the dominant influence. These results emphasize the importance of local permeability on diffuse hydrothermal venting at mid-ocean ridges and the need to better quantify heat flux associated with young oceanic crust.

  10. 3D imaging of vents and sand injectites produced by Lower Cretaceous hydrothermal activity in the southern North Sea

    DEFF Research Database (Denmark)

    Moreau, Julien

    : size, shape, upper part shape and source bed. Most of the vents originate from the Lower Germanic Triassic Group (Bundsandstein). They are conical in shape and have an eye-shaped upper dome. At the top, numerous domes have been eroded. A few domes have jacked up the overburden, sometimes including...... lateral intrusion (sandstone sill). Almost all vents terminate at the Upper Jurassic layers. Based on the relationship with the overlaying deposits, we have constrained the time span of venting to be from pre- Mid Hauterivian times (eroded/partly eroded upper parts of the vents) to Albian times for the...... isolated Zuidal volcano situated onshore (50 km East and older). Besides the economic impact of this discovery, one could ask what is the trigger of this activity? Was it so discreet in the seismic images that it is more widespread than thought in the basin? The relation with the tectonic context is still...

  11. Low-pH waters discharging from submarine vents at Panarea Island (Aeolian Islands, southern Italy) after the 2002 gas blast: origin of hydrothermal fluids and implications for volcanic surveillance

    OpenAIRE

    Tassi, F.; Dept. of Earth Sciences, Univ. of Florence, Via G. La Pira, 4, 50121 Florence, Italy; Capaccioni, B.; Dept. of Earth and Geological-Environmental Sciences, Univ. of Bologna, P.zza di Porta S. Donato, 40127 Bologna, Italy; Caramanna, G.; Department of Earth Sciences, University of Rome 'La Sapienza', P.le Aldo Moro,5 - 00185 Rome, Italy; Cinti, D.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Montegrossi, G.; CNR-Institute of Geosciences and Earth Resources; Pizzino, L.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Quattrocchi, F.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Vaselli, O.; Dept. of Earth Sciences, Univ. of Florence, Via G. La Pira, 4, 50121 Florence, Italy

    2008-01-01

    A geochemical survey of thermal waters collected from submarine vents at Panarea Island (Aeolian Islands, southern Italy) was carried out from December 2002 to March 2007, in order to investigate i) the geochemical processes controlling the chemical composition of the hydrothermal fluids and ii) the possible relations between the chemical features of the hydrothermal reservoir and the activity of the magmatic system. Compositional data of the thermal water samples were integrated in a hydrolo...

  12. Low-pH waters discharging from submarine vents at Panarea Island (Aeolian Islands, southern Italy) after the 2002 gas blast: origin of hydrothermal fluids and implications for volcanic surveillance.

    OpenAIRE

    Tassi, F.; Dept. of Earth Sciences, Univ. of Florence, Via G. La Pira, 4, 50121 Florence, Italy; Capaccioni, B.; Dept. of Earth and Geological-Environmental Sciences, Univ. of Bologna, P.zza di Porta S. Donato, 40127 Bologna, Italy; Caramanna, G.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Cinti, D.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Montegrossi, G.; CNR-Institute of Geosciences and Earth Resources; Pizzino, L.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Quattrocchi, F.; Istituto Nazionale di Geofisica e Vulcanologia, Sezione Roma1, Roma, Italia; Vaselli, O.; Dept. of Earth Sciences, Univ. of Florence, Via G. La Pira, 4, 50121 Florence, Italy

    2007-01-01

    A geochemical survey of thermal waters collected from submarine vents at Panarea Island (Aeolian Islands, southern Italy) was carried out from December 2002 to March 2007, in order to investigate i) the geochemical processes controlling the chemical composition of the hydrothermal fluids and ii) the possible relations between the chemical features of the hydrothermal reservoir and the activity of the magmatic system. Compositional data of the thermal water samples were integrated in a hydrolo...

  13. The Lost City hydrothermal system: Constraints imposed by vent fluid chemistry and reaction path models on subseafloor heat and mass transfer processes

    Science.gov (United States)

    Seyfried, W. E.; Pester, Nicholas J.; Tutolo, Benjamin M.; Ding, Kang

    2015-08-01

    Since the first reported discovery of the Lost City hydrothermal system in 2001, it was recognized that seawater alteration of ultramafic rocks plays a key role in the composition of the coexisting vent fluids. The unusually high pH and high concentrations of H2 and CH4 provide compelling evidence for this. Here we report the chemistry of hydrothermal fluids sampled from two vent structures (Beehive: ∼90-116 °C, and M6: ∼75 °C) at Lost City in 2008 during cruise KNOX18RR using ROV Jason 2 and R/V Revelle assets. The vent fluid chemistry at both sites reveals considerable overlap in concentrations of dissolved gases (H2, CH4), trace elements (Cs, Rb, Li, B and Sr), and major elements (SO4, Ca, K, Na, Cl), including a surprising decrease in dissolved Cl, suggesting a common source fluid is feeding both sites. The absence of Mg and relatively high concentrations of Ca and sulfate suggest solubility control by serpentine-diopside-anhydrite, while trace alkali concentrations, especially Rb and Cs, are high, assuming a depleted mantle protolith. In both cases, but especially for Beehive vent fluid, the silica concentrations are well in excess of those expected for peridotite alteration and the coexistence of serpentine-brucite at all reasonable temperatures. However, both the measured pH and silica values are in better agreement with serpentine-diopside-tremolite-equilibria. Geochemical modeling demonstrates that reaction of plagioclase with serpentinized peridotite can shift the chemical system away from brucite and into the tremolite stability field. This is consistent with the complex intermingling of peridotite and gabbroic bodies commonly observed within the Atlantis Massif. We speculate the existence of such plagioclase bearing peridotite may also account for the highly enriched trace alkali (Cs, Rb) concentrations in the Lost City vent fluids. Additionally, reactive transport modeling taking explicit account of temperature dependent rates of mineral

  14. Temporal evolution of magmatic-hydrothermal systems in the Manus Basin, Papua New Guinea: Insights from vent fluid chemistry and bathymetric observations

    Science.gov (United States)

    Reeves, E. P.; Thal, J.; Schaen, A.; Ono, S.; Seewald, J.; Bach, W.

    2015-12-01

    The temporal evolution of hydrothermal fluids from back-arc systems is poorly constrained, despite growing evidence for dynamic magmatic-hydrothermal activity, and imminent commercial mining. Here we discuss surveys of diverse vent fluids from multiple hydrothermal fields in the Manus back-arc basin, Papua New Guinea, sampled in 2006 and 2011. Effects of host rock composition, and dynamic magmatic volatile inputs on fluid chemistry are evaluated to understand changes in these systems. Highly acidic and SO4-rich moderate temperature fluids (~48-215°C), as well as SO4-poor black smoker fluids (up to 358°C), were collected at the PACMANUS, SuSu Knolls and DESMOS areas in 2006 and 2011. Acidic, milky white SuSu and DESMOS fluids, rich in elemental S and SO4, exit the seafloor with Na, K, Mg, and Ca diluted conservatively up to 30% relative to seawater, implying subsurface mixing of seawater with SO2-rich aqueous fluids exsolved from magma, analogous to subaerial fumarole discharge. SO2 disproportionation during cooling and mixing of magmatic fluids contributes acidity, SO4, H2S and S(0)(s), as well as widespread S outcrops on the seafloor. Nearby black smoker fluids indicate entrainment and reaction of magmatic fluid into convecting fluids at depth, and additional hybrid-type fluids appear to consist of evolved seawater and unreacted magmatic fluid SO2 derivatives. Fluids at DESMOS in 2006 indicate increased magmatic SO2 relative to 1995, despite constant low venting temperatures (~120°C). In contrast, dramatic changes in bathymetry and seafloor morphology point to substantial continuous eruption of volcaniclastic material between 2006 and 2011 at SuSu Knolls, burying fumarolic vents from 2006. Compositions of new 2011 acidic, sulfate-rich fluids there suggest reaction with less altered, fresher rock. At the PACMANUS area, farther from the arc, direct magmatic degassing to the seafloor is not occurring presently, but entrainment and reaction of similar acid

  15. Novel Barite Chimneys at the Loki's Castle Vent Field Shed Light on Key Factors Shaping Microbial Communities and Functions in Hydrothermal Systems

    Science.gov (United States)

    Steen, Ida H.; Dahle, Håkon; Stokke, Runar; Roalkvam, Irene; Daae, Frida-Lise; Rapp, Hans Tore; Pedersen, Rolf B.; Thorseth, Ingunn H.

    2016-01-01

    In order to fully understand the cycling of elements in hydrothermal systems it is critical to understand intra-field variations in geochemical and microbiological processes in both focused, high-temperature and diffuse, low-temperature areas. To reveal important causes and effects of this variation, we performed an extensive chemical and microbiological characterization of a low-temperature venting area in the Loki's Castle Vent Field (LCVF). This area, located at the flank of the large sulfide mound, is characterized by numerous chimney-like barite (BaSO4) structures (≤ 1 m high) covered with white cotton-like microbial mats. Results from geochemical analyses, microscopy (FISH, SEM), 16S rRNA gene amplicon-sequencing and metatranscriptomics were compared to results from previous analyses of biofilms growing on black smoker chimneys at LCVF. Based on our results, we constructed a conceptual model involving the geochemistry and microbiology in the LCVF. The model suggests that CH4 and H2S are important electron donors for microorganisms in both high-temperature and low-temperature areas, whereas the utilization of H2 seems restricted to high-temperature areas. This further implies that sub-seafloor processes can affect energy-landscapes, elemental cycling, and the metabolic activity of primary producers on the seafloor. In the cotton-like microbial mats on top of the active barite chimneys, a unique network of single cells of Epsilonproteobacteria interconnected by threads of extracellular polymeric substances (EPS) was seen, differing significantly from the long filamentous Sulfurovum filaments observed in biofilms on the black smokers. This network also induced nucleation of barite crystals and is suggested to play an essential role in the formation of the microbial mats and the chimneys. Furthermore, it illustrates variations in how different genera of Epsilonproteobacteria colonize and position cells in different vent fluid mixing zones within a vent field

  16. Hydrothermal methane venting between 12°N and 26°N along the Mid-Atlantic Ridge

    OpenAIRE

    Charlou, Jean-Luc; Donval, Jean-Pierre

    1993-01-01

    Hydrographic surveys along the Mid-Atlantic Ridge (MAR) between 12°N and 26°N, carried out from 1984 to 1990, show a variable pattern of CH4-rich water column plumes. The vertical distribution of CH4 at stations located every 20–40 km is presented along this 1200-km-long section of the MAR. CH4 venting is clearly demonstrated. CH4-enriched fluids rise from vents as plumes; spreading is confined to the axial valley due to the topography of the MAR. CH4 contents from 45 nmol to 675 nmol/kg are ...

  17. Bacterial diversity and their adaptations in the shallow water hydrothermal vent at D. Joao de Castro Seamount (DJCS), Azores, Portugal

    Digital Repository Service at National Institute of Oceanography (India)

    Mohandass, C.; Rajasabapathy, R.; Ravindran, C.; Colaco, A.; Santos, R.S.; Meena, R.M.

    ). Eighty six percent of the strains isolated from the shallow vent belonged to Gram-positive and spore producers. Most of the colonies were circular and creamy or dull white and only one is reported to be yellow in colour. Ninety percent of the strains.... Colony characteristics of various isolates obtained from the vent: Culture Spore Gram ± Colour Size Elevation H 2 S Motility SV1 + - Cream 5mm Convex - Motile SV2 + + Cream 4mm Convex + Non- motile SV3 + + Off white Pinpoint Raised - Motile SV4...

  18. Assessment of integrated process based on hydrothermal and alkaline treatments for enzymatic saccharification of sweet sorghum stems.

    Science.gov (United States)

    Sun, Shao-Long; Sun, Shao-Ni; Wen, Jia-Long; Zhang, Xue-Ming; Peng, Feng; Sun, Run-Cang

    2014-10-29

    In this study, sweet sorghum stem was subjected to hydrothermal pretreatment (HTP) and alkaline post-treatment to enhance its saccharification ratio by reducing its recalcitrance. The results showed that the HTP (110-210°C, 0.5-2.0h) significantly degraded hemicelluloses, and the pretreatment at the temperature higher than 190°C led to the partial degradation of the cellulose. As compared to the sole HTP, the integrated process removed most of lignin and hemicelluloses, which incurred a higher cellulose saccharification ratio. Under an optimum condition evaluated (HTP at 170°C for 0.5h and subsequent 2% NaOH treatment), 77.5% saccharification ratio was achieved, which was 1.8, 2.0 and 5.5 times as compared to the only HTP pretreated substrates, alkaline treated substrates alone and the raw material without pretreatment, respectively. Clearly, the integrated process can be considered as a promising approach to achieve an efficient conversion of lignocellulose to fermentable glucose. PMID:25459857

  19. Morphology of First Zoeal Stage of Four Genera of Alvinocaridid Shrimps from Hydrothermal Vents and Cold Seeps: Implications for Ecology, Larval Biology and Phylogeny.

    Directory of Open Access Journals (Sweden)

    Iván Hernández-Ávila

    Full Text Available Alvinocaridid shrimps are endemic species inhabiting hydrothermal vents and/or cold seeps. Although indirect evidences (genetic and lipid markers suggest that their larval stages disperse widely and support large scale connectivity, larval life and mechanisms underlying dispersal are unknown in alvinocaridids. Here we provide for the first time detailed descriptions of the first larval stage (zoea I of four alvinocaridid species: Rimicaris exoculata and Mirocaris fortunata from the Mid-Atlantic Ridge, Alvinocaris muricola from the Congo Basin and Nautilocaris saintlaurentae from the Western Pacific. The larvae were obtained from onboard hatching of brooding females (either at atmospheric pressure or at habitat pressure in hyperbaric chambers and from the water column near adult habitats, sampled with plankton pumps or sediment traps. Major characteristics of the alvinocaridid larvae include undeveloped mandible and almost complete absence of setation in the inner margin of the mouth parts and maxillipeds. Although the larvae are very similar between the four species studied, some morphological features could be used for species identification. In addition, undeveloped mouthparts and the large amount of lipid reserves strongly support the occurrence of primary lecithotrophy in the early stage of alvinocaridids. Although lecithotrophy in decapod crustaceans is usually associated with abbreviated larval development, as a mechanism of larval retention, morphological and physiological evidences suggest the occurrence of an extended and lecithotrophic larval stage in the Alvinocarididae. These traits permit the colonization of widely dispersed and fragmented environments of hydrothermal vents and cold seeps. Distribution of larval traits along the phylogenetic reconstruction of the Alvinocarididae and related families suggest that lecithotrophy/planktotrophy and extended/abbreviated development have evolved independently along related families in all

  20. Vallitalea pronyensis sp nova, isolated from a marine alkaline hydrothermal chimney

    OpenAIRE

    Ben Aissa, F.; Postec, A.; Erauso, G.; Payri, Claude; Pelletier, Bernard; Hamdi, M.; Ollivier, Bernard; Fardeau, Marie-Laure

    2014-01-01

    A novel thermotolerant, anaerobic, Gram-stain-positive, spore-forming bacterium was isolated from a hydrothermal chimney in Prony Bay, New Caledonia. This strain, designated FatNl3(T), grew at 15-55 degrees C (optimum 30 degrees C) and at pH 5.8-8.9 (optimum 7.7). It was slightly halophilic, requiring at least 0.5% NaCl for growth (optimum 2.5-3.0 %), and was able to grow at up to 6% NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron ...

  1. 贵州镇宁乐纪泥盆系重晶石矿热水沉积特征%Hydrothermal Venting-Flowing Sedimentation Characteristics of Devonian Barite Deposits from Leji,Zhenning County,Guizhou Province

    Institute of Scientific and Technical Information of China (English)

    高军波; 杨瑞东; 陶平; 魏怀瑞; 刘坤

    2012-01-01

    The Devonian barite deposits located in Zhenning County,Guizhou Province have proved reserves of 33.02×106 t,belonging to large-scale ore deposits.Research on sedimentation structures and tectonic characteristics related to the barite deposits reveal the coexistence of a variety of structures like laminar structures,barite-banded structure,brecciated structure,barite-fragmental structure and massive structure,etc.,which are similar to those of the Tianzhu-Dahebian barite deposit,the hydrothermal barite rocks of Gutan in Guangxi Province and the hydrothermal water sedimentary barite rocks of Erlangping Group from north Qinling Mountains.Several types of sedimentary characteristics,such as hydrothermal venting channel phases formed in submarine hydrothermal venting,unitary hydrothermal lithofacies and hydrothermally mixed synsedimentary phases,were identified widely in barite orebeds in Zhenning,Guizhou.Similar to Devonian hydrothermal sedimentary rocks(deposits) from Qinling,those sedimentary phases and lithofacies features found in Zhenning characterize a hydrothermal turbidity current sedimentation phase.Sedimentary structures,constructions and lithofacies features of the orebed record well the information of submarine hydrothermal venting and its evolution,the sedimentation rhythm characteristics reveal the intensity of hydrothermal venting-flowing was weak-strong-weak.Hence,barite deposits in Zhenning were formed in submarine hydrothermal venting sedimentation,and the hydrothermal sedimentary structures,constructions and hydrothermal lithofacies features make them ideal places for research on ancient hydrothermal venting diagenesis,metallogenesis and hydrothermal lithofacies categorization.%贵州镇宁乐纪泥盆系重晶石矿床地质储量3 302万t,属大型矿床类型。通过对矿床沉积结构、构造特征研究发现,矿床(体)中分布:纹层状构造、条带状构造、角砾状构造、碎屑状构造、块状构造等,它们与天

  2. Free-living bacterial communities associated with tubeworm (Ridgeia piscesae) aggregations in contrasting diffuse flow hydrothermal vent habitats at the Main Endeavour Field, Juan de Fuca Ridge.

    Science.gov (United States)

    Forget, Nathalie L; Kim Juniper, S

    2013-04-01

    We systematically studied free-living bacterial diversity within aggregations of the vestimentiferan tubeworm Ridgeia piscesae sampled from two contrasting flow regimes (High Flow and Low Flow) in the Endeavour Hydrothermal Vents Marine Protected Area (MPA) on the Juan de Fuca Ridge (Northeast Pacific). Eight samples of particulate detritus were recovered from paired tubeworm grabs from four vent sites. Most sequences (454 tag and Sanger methods) were affiliated to the Epsilonproteobacteria, and the sulfur-oxidizing genus Sulfurovum was dominant in all samples. Gammaproteobacteria were also detected, mainly in Low Flow sequence libraries, and were affiliated with known methanotrophs and decomposers. The cooccurrence of sulfur reducers from the Deltaproteobacteria and the Epsilonproteobacteria suggests internal sulfur cycling within these habitats. Other phyla detected included Bacteroidetes, Actinobacteria, Chloroflexi, Firmicutes, Planctomycetes, Verrucomicrobia, and Deinococcus-Thermus. Statistically significant relationships between sequence library composition and habitat type suggest a predictable pattern for High Flow and Low Flow environments. Most sequences significantly more represented in High Flow libraries were related to sulfur and hydrogen oxidizers, while mainly heterotrophic groups were more represented in Low Flow libraries. Differences in temperature, available energy for metabolism, and stability between High Flow and Low Flow habitats potentially explain their distinct bacterial communities. PMID:23401293

  3. Colonization of plant substrates at hydrothermal vents and cold seeps in the northeast Atlantic and Mediterranean and occurrence of symbiont-related bacteria.

    Science.gov (United States)

    Szafranski, Kamil M; Deschamps, Philippe; Cunha, Marina R; Gaudron, Sylvie M; Duperron, Sébastien

    2015-01-01

    Reducing conditions with elevated sulfide and methane concentrations in ecosystems such as hydrothermal vents, cold seeps or organic falls, are suitable for chemosynthetic primary production. Understanding processes driving bacterial diversity, colonization and dispersal is of prime importance for deep-sea microbial ecology. This study provides a detailed characterization of bacterial assemblages colonizing plant-derived substrates using a standardized approach over a geographic area spanning the North-East Atlantic and Mediterranean. Wood and alfalfa substrates in colonization devices were deployed for different periods at 8 deep-sea chemosynthesis-based sites in four distinct geographic areas. Pyrosequencing of a fragment of the 16S rRNA-encoding gene was used to describe bacterial communities. Colonization occurred within the first 14 days. The diversity was higher in samples deployed for more than 289 days. After 289 days, no relation was observed between community richness and deployment duration, suggesting that diversity may have reached saturation sometime in between. Communities in long-term deployments were different, and their composition was mainly influenced by the geographical location where devices were deployed. Numerous sequences related to horizontally-transmitted chemosynthetic symbionts of metazoans were identified. Their potential status as free-living forms of these symbionts was evaluated based on sequence similarity with demonstrated symbionts. Results suggest that some free-living forms of metazoan symbionts or their close relatives, such as Epsilonproteobacteria associated with the shrimp Rimicaris exoculata, are efficient colonizers of plant substrates at vents and seeps. PMID:25774156

  4. Testing biological control of colonization by vestimentiferan tubeworms at deep-sea hydrothermal vents (East Pacific Rise, 9°50'N)1

    Science.gov (United States)

    Hunt, Heather L.; Metaxas, Anna; Jennings, Robert M.; Halanych, Kenneth M.; Mullineaux, Lauren S.

    2004-02-01

    Three species of vestimentiferans are found at hydrothermal vents on the East Pacific Rise (EPR). Tevnia jerichonana is an early colonist and Riftia pachyptila has the greatest biomass in established vent assemblages, but the role of Oasisia alvinae, a small species that occurs sporadically, is unknown. Anecdotal evidence suggests that O. alvinae may be abundant in the microhabitat underneath mussels. Previous studies have suggested that early T. jerichonana colonists may facilitate settlement of the late colonist R. pachyptila. To address potential mechanisms for the successional sequence and to explore the role of O. alvinae, we examined the effects of the presence of vestimentiferan ( R. pachyptila and T. jerichonana) tubes and mussel ( Bathymodiolus thermophilis) shell cover on recruitment of vestimentiferans on basalt blocks deployed at 9°50'N, 104°17'W on the EPR. A molecular assay was used to identify individuals to species since they were too small to be identified morphologically. Although colonists in both experiments belonged to all three species of vestimentiferans, only a few were T. jerichonana. Colonization of vestimentiferans did not increase in the presence of vestimentiferan tubes. The presence of mussel shell cover did not influence the proportions of R. pachyptila and O. alvinae, or the total number of colonists. Because the experimental blocks in this study were placed within dense clumps of R. pachyptila, we suggest that, while T. jerichonana may be an important cue for vestimentiferans settling at new vents, adult R. pachyptila also can act as a settlement cue for larvae. O. alvinae colonists were abundant in all of the treatments in our experiments, indicating that, although adults of this species are apparently rare at these sites, O. alvinae can settle in abundance if a suitable micro-environment is available.

  5. Formation of Lactic Acid from Cellulosic Biomass by Alkaline Hydrothermal Reaction

    Science.gov (United States)

    Yan, X.; Jini, F.; Kishita, A.; Enomoto, H.; Tohji, K.

    2008-02-01

    Glucose, as a model compound of cellulosic biomass, was used as a test material. Ca(OH)2 and NaOH were selected as alkali. Results showed that both NaOH and Ca(OH)2, can promote the formation of lactic acid in a hydrothermal reaction of glucose. In the case of the addition of NaOH, lactic acid was obtained with a good yield of 27% based on a carbon base at 300 °C for 60 s with a NaOH concentration of 2.5 M. In the case of the addition of Ca(OH)2, the highest yield of lactic acid is 20%, which occurred at 300 °C for 60 s with a Ca(OH)2 concentration of 0.32 M. The formation mechanisms of lactic acid from glucose were also discussed according to intermediate products identified. Lactic acid may be generated via formaldehyde, glycolaldehyde besides via the aldose having three carbon atoms in hydrothermal reaction which all formed by the reverse aldol condensation of hexoses.

  6. Development and field application of a 6-bottle serial gas-tight fluid sampler for collecting seafloor cold seep and hydrothermal vent fluids with autonomous operation capability

    Science.gov (United States)

    Wu, S.; Ding, K.; Yang, C.; Seyfried, W. E., Jr.; Tan, C.; Schaen, A. T.; Luhmann, A. J.

    2014-12-01

    A 6-bottle serial gas-tight sampler (so-called "six-shooter") was developed for application with deep-sea vent fluids. The new device is composed of a custom-made 6-channel valve manifold and six sampling bottles which are circularly distributed around the valve manifold. Each valve channel consists of a high-pressure titanium cartridge valve and a motor-driven actuator. A sampling snorkel is connected to the inlet of the manifold that delivers the incoming fluid to different bottles. Each sampling bottle has a 160 ml-volume chamber and an accumulator chamber inside where compressed nitrogen is used to maintain the sample at near in-situ pressure. An electronics chamber that is located at the center of the sampler is used to carry out all sampling operations, autonomously, if desired. The sampler is of a compact circular configuration with a diameter of 26 cm and a length of 54 cm. During the SVC cruise AT 26-12, the sampler was deployed by DSV2 Alvin at a cold seep site MC036 with a depth of 1090 m in the Gulf of Mexico. The sampler collected fluid samples automatically following the tidal cycle to monitor the potential impact of the tide cycle on the fluid chemistry of cold seep in a period of two day. During the cruise AT 26-17, the sampler was used with newly upgraded DSV2 Alvin three times at the hydrothermal vent sites along Axial Seamount and Main Endeavor Field on Juan de Fuca Ridge. During a 4-day deployment at Anemone diffuse site (Axial Caldera), the sampler was set to work in an autonomous mode to collect fluid samples according to the preset interval. During other dives, the sampler was manually controlled via ICL (Inductively Coupled Link) communication through the hull. Gas-tight fluid samples were collected from different hydrothermal vents with temperatures between 267 ℃ and 335 ℃ at the depth up to 2200 m. The field results indicate unique advantages of the design. It can be deployed in extended time period with remote operation or working

  7. Biogeography of deep-sea wood fall, cold seep and hydrothermal vent Ostracoda (Crustacea), with the description of a new family and a taxonomic key to living Cytheroidea

    Science.gov (United States)

    Karanovic, Ivana; Brandão, Simone Nunes

    2015-01-01

    Stimulated by finding a novel cytheroid ostracod in a piece of sunken wood retrieved from the sea-bed in the Kuril-Kamchatka Trench, we have reviewed all previously published data on ostracods from similarly ephemeral deep-sea habitats (wood falls, hydrothermal vents and cold seeps). These data are placed in the context of all data on living, deep-sea ostracods from other environments. We confirm previous authors' conclusions that faunas from these ephemeral habitats are similar at the generic level, and include elements common to shallow and deep habitats. However, at the species level, endemism varies from zero at cold seeps, to 35% in wood falls and 60% at hydrothermal vents, which is an indication of the relative longevity of these habitats. Non-endemic species occur also in oligotrophic, deep-sea sediments but not in shallow environments. This is in contradiction to previous assumptions that these ephemeral faunas share more species and with shallow habitats than genera with the oligotrophic, deep-sea sediments. We agree with previous authors that the dispersal strategy of wood fall, vent and seep ostracods includes hitchhiking and we propose that it also includes the ability to survive ingestion by larger, more motile animals. The homogeneity of the faunas from ephemeral habitats collected off the American continent is in stark contrast to the highly endemic fauna found in Northwestern Pacific. This suggests that the ostracods may have biogeographical patterns similar to those previously proposed for other groups of benthos. However, any proposal for a global biogeographical scheme for ostracod distributions will have to await far more comprehensive coverage from presently unstudied regions. Finally, we describe and name a novel species of ostracod from the wood fall collected at a depth of 5229 m in the abyss east to the Kuril-Kamchatka Trench, Northwestern Pacific; erecting a new family Keysercytheridae fam. nov. and a new genus, Keysercythere gen. nov., to

  8. Effects of alkaline precipitating agents on synthesis of magnetite nanomaterials by hydrothermal d-glucose method

    Energy Technology Data Exchange (ETDEWEB)

    Zhang Wei, E-mail: zhangwei.hebut@gmail.com [Hebei University of Technology, Engineering Research Center of Seawater Utilization Technology, Ministry of Education (China); Jia Shaoyi; Wu Songhai [Tianjin University, School of Chemical Engineering and Technology (China); Zhang Shaofeng; Liu Yan [Hebei University of Technology, Engineering Research Center of Seawater Utilization Technology, Ministry of Education (China)

    2013-06-15

    Pure magnetite (Fe{sub 3}O{sub 4}) nanoparticles and carbon-coated magnetite (Fe{sub 3}O{sub 4}/C) nanocomposites were facilely synthesized in a hydrothermal reaction system by changing sodium hydroxide and sodium acetate as the precipitating agents, respectively. The sizes of pure Fe{sub 3}O{sub 4} nanoparticles and Fe{sub 3}O{sub 4}/C nanocomposites with core-shell structure were varied from 10 to 120 nm, and the magnetic properties of these two samples performed as superparamagnetic and ferrimagnetic. A possible formation mechanism for these two types of magnetite nanomaterials was discussed. It was the different alkali environments provided by the precipitating agents that led to the varieties in morphology, structure, and property of the final magnetite nanomaterials.

  9. Effects of alkaline precipitating agents on synthesis of magnetite nanomaterials by hydrothermal d-glucose method

    International Nuclear Information System (INIS)

    Pure magnetite (Fe3O4) nanoparticles and carbon-coated magnetite (Fe3O4/C) nanocomposites were facilely synthesized in a hydrothermal reaction system by changing sodium hydroxide and sodium acetate as the precipitating agents, respectively. The sizes of pure Fe3O4 nanoparticles and Fe3O4/C nanocomposites with core–shell structure were varied from 10 to 120 nm, and the magnetic properties of these two samples performed as superparamagnetic and ferrimagnetic. A possible formation mechanism for these two types of magnetite nanomaterials was discussed. It was the different alkali environments provided by the precipitating agents that led to the varieties in morphology, structure, and property of the final magnetite nanomaterials.

  10. Hydrocarbons and oxidized organic compounds in hydrothermal fluids from Rainbow and Lost City ultramafic-hosted vents

    NARCIS (Netherlands)

    Konn, C.; Charlou, J.L.; Donval, J.P.; Holm, N.G.; Dehairs, F.; Bouillon, S.

    2009-01-01

    The first building blocks of life could be produced in ultramafic-hosted hydrothermal systems considering the large amounts of hydrogen and methane generated by serpentinisation and Fischer–Tropsch-Type synthesis, respectively, in those systems. The purpose of this study was to detect and characteri

  11. Group-specific 16S rRNA-targeted oligonucleotide probes to identify thermophilic bacteria in marine hydrothermal vents

    NARCIS (Netherlands)

    Harmsen, HJM; Prieur, D; Jeanthon, C

    1997-01-01

    Four 16S rRNA-targeted oligonucleotide probes were designed for the detection of thermophilic members of the domain Bacteria known to thrive in marine hydrothermal systems, We developed and characterized probes encompassing most of the thermophilic members of the genus Bacillus, most species of the

  12. Ultra-diffuse hydrothermal venting supports Fe-oxidizing bacteria and massive umber deposition at 5000 m off Hawaii.

    Science.gov (United States)

    Edwards, Katrina J; Glazer, B T; Rouxel, O J; Bach, W; Emerson, D; Davis, R E; Toner, B M; Chan, C S; Tebo, B M; Staudigel, H; Moyer, C L

    2011-11-01

    A novel hydrothermal field has been discovered at the base of Lōihi Seamount, Hawaii, at 5000 mbsl. Geochemical analyses demonstrate that 'FeMO Deep', while only 0.2 °C above ambient seawater temperature, derives from a distal, ultra-diffuse hydrothermal source. FeMO Deep is expressed as regional seafloor seepage of gelatinous iron- and silica-rich deposits, pooling between and over basalt pillows, in places over a meter thick. The system is capped by mm to cm thick hydrothermally derived iron-oxyhydroxide- and manganese-oxide-layered crusts. We use molecular analyses (16S rDNA-based) of extant communities combined with fluorescent in situ hybridizations to demonstrate that FeMO Deep deposits contain living iron-oxidizing Zetaproteobacteria related to the recently isolated strain Mariprofundus ferroxydans. Bioenergetic calculations, based on in-situ electrochemical measurements and cell counts, indicate that reactions between iron and oxygen are important in supporting chemosynthesis in the mats, which we infer forms a trophic base of the mat ecosystem. We suggest that the biogenic FeMO Deep hydrothermal deposit represents a modern analog for one class of geological iron deposits known as 'umbers' (for example, Troodos ophilolites, Cyprus) because of striking similarities in size, setting and internal structures. PMID:21544100

  13. Molecular identification of differentially regulated genes in the hydrothermal-vent species Bathymodiolus thermophilus and Paralvinella pandorae in response to temperature

    Directory of Open Access Journals (Sweden)

    Shillito Bruce

    2009-05-01

    Full Text Available Abstract Background Hydrothermal vents and cold seeps represent oases of life in the deep-sea environment, but are also characterized by challenging physical and chemical conditions. The effect of temperature fluctuations on vent organisms in their habitat has not been well explored, in particular at a molecular level, most gene expression studies being conducted on coastal marine species. In order to better understand the response of hydrothermal organisms to different temperature regimes, differentially expressed genes (obtained by a subtractive suppression hybridization approach were identified in the mussel Bathymodiolus thermophilus and the annelid Paralvinella pandorae irlandei to characterize the physiological processes involved when animals are subjected to long term exposure (2 days at two contrasting temperatures (10° versus 20°C, while maintained at in situ pressures. To avoid a potential effect of pressure, the experimental animals were initially thermally acclimated for 24 hours in a pressurized vessel. Results For each species, we produced two subtractive cDNA libraries (forward and reverse from sets of deep-sea mussels and annelids exposed together to a thermal challenge under pressure. RNA extracted from the gills, adductor muscle, mantle and foot tissue were used for B. thermophilus. For the annelid model, whole animals (small individuals were used. For each of the four libraries, we sequenced 200 clones, resulting in 78 and 83 unique sequences in mussels and annelids (about 20% of the sequencing effort, respectively, with only half of them corresponding to known genes. Real-time PCR was used to validate differentially expressed genes identified in the corresponding libraries. Strong expression variations have been observed for some specific genes such as the intracellular hemoglobin, the nidogen protein, and Rab7 in P. pandorae, and the SPARC protein, cyclophilin, foot protein and adhesive plaque protein in B. thermophilus

  14. Culture-Independent Identification of Manganese-Oxidizing Genes from Deep-Sea Hydrothermal Vent Chemoautotrophic Ferromanganese Microbial Communities Using a Metagenomic Approach

    Science.gov (United States)

    Davis, R.; Tebo, B. M.

    2013-12-01

    Microbial activity has long been recognized as being important to the fate of manganese (Mn) in hydrothermal systems, yet we know very little about the organisms that catalyze Mn oxidation, the mechanisms by which Mn is oxidized or the physiological function that Mn oxidation serves in these hydrothermal systems. Hydrothermal vents with thick ferromanganese microbial mats and Mn oxide-coated rocks observed throughout the Pacific Ring of Fire are ideal models to study the mechanisms of microbial Mn oxidation, as well as primary productivity in these metal-cycling ecosystems. We sampled ferromanganese microbial mats from Vai Lili Vent Field (Tmax=43°C) located on the Eastern Lau Spreading Center and Mn oxide-encrusted rhyolytic pumice (4°C) from Niua South Seamount on the Tonga Volcanic Arc. Metagenomic libraries were constructed and assembled from these samples and key genes known to be involved in Mn oxidation and carbon fixation pathways were identified in the reconstructed genomes. The Vai Lili metagenome assembled to form 121,157 contiguous sequences (contigs) greater than 1000bp in length, with an N50 of 8,261bp and a total metagenome size of 593 Mbp. Contigs were binned using an emergent self-organizing map of tetranucleotide frequencies. Putative homologs of the multicopper Mn-oxidase MnxG were found in the metagenome that were related to both the Pseudomonas-like and Bacillus-like forms of the enzyme. The bins containing the Pseudomonas-like mnxG genes are most closely related to uncultured Deltaproteobacteria and Chloroflexi. The Deltaproteobacteria bin appears to be an obligate anaerobe with possible chemoautotrophic metabolisms, while the Chloroflexi appears to be a heterotrophic organism. The metagenome from the Mn-stained pumice was assembled into 122,092 contigs greater than 1000bp in length with an N50 of 7635 and a metagenome size of 385 Mbp. Both forms of mnxG genes are present in this metagenome as well as the genes encoding the putative Mn

  15. Magnetite formation from ferrihydrite by hyperthermophilic archaea from Endeavour Segment, Juan de Fuca Ridge hydrothermal vent chimneys.

    Science.gov (United States)

    Lin, T Jennifer; Breves, E A; Dyar, M D; Ver Eecke, H C; Jamieson, J W; Holden, J F

    2014-05-01

    Hyperthermophilic iron reducers are common in hydrothermal chimneys found along the Endeavour Segment in the northeastern Pacific Ocean based on culture-dependent estimates. However, information on the availability of Fe(III) (oxyhydr) oxides within these chimneys, the types of Fe(III) (oxyhydr) oxides utilized by the organisms, rates and environmental constraints of hyperthermophilic iron reduction, and mineral end products is needed to determine their biogeochemical significance and are addressed in this study. Thin-section petrography on the interior of a hydrothermal chimney from the Dante edifice at Endeavour showed a thin coat of Fe(III) (oxyhydr) oxide associated with amorphous silica on the exposed outer surfaces of pyrrhotite, sphalerite, and chalcopyrite in pore spaces, along with anhydrite precipitation in the pores that is indicative of seawater ingress. The iron sulfide minerals were likely oxidized to Fe(III) (oxyhydr) oxide with increasing pH and Eh due to cooling and seawater exposure, providing reactants for bioreduction. Culture-dependent estimates of hyperthermophilic iron reducer abundances in this sample were 1740 and 10 cells per gram (dry weight) of material from the outer surface and the marcasite-sphalerite-rich interior, respectively. Two hyperthermophilic iron reducers, Hyperthermus sp. Ro04 and Pyrodictium sp. Su06, were isolated from other active hydrothermal chimneys on the Endeavour Segment. Strain Ro04 is a neutrophilic (pH opt 7-8) heterotroph, while strain Su06 is a mildly acidophilic (pH opt 5), hydrogenotrophic autotroph, both with optimal growth temperatures of 90-92 °C. Mössbauer spectroscopy of the iron oxides before and after growth demonstrated that both organisms form nanophase (hydrothermal systems that are mildly acidic where mineral weathering at increased temperatures occurs. PMID:24612368

  16. A Special Issue (Part-II): Mafic-ultramafic rocks and alkaline-carbonatitic magmatism and associated hydrothermal mineralization - dedication to Lia Nikolaevna Kogarko

    OpenAIRE

    Kogarko Lia N.; Gwalani Lalchand G.; Downes Peter J.; Randive Kirtikumar R.

    2015-01-01

    This is the second part of a two-volumespecial issue of Open Geoscience (formerly Central European Journal of Geosciences) that aims to be instrumental in providing an update of Mafic-Ultramafic Rocks and Alkaline- Carbonatitic Magmatism and Associated Hydrothermal Mineralization. Together, these two volumes provide a detailed and comprehensive coverage of the subjects that are relevant to the research work of P.Comin-Chiaramonti (Italy) and LiaN. Kogarko (Russia) towh...

  17. Degradation of Rhodamine B Dye by TiO2 Nanotubes Photocatalyst Synthesized via Alkaline Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    T Mazlina Mat Darus

    2015-01-01

    Full Text Available Titanium dioxide (TiO2 nanotubes have successfully been synthesized by using one step approach of hydrothermal method. Commercial TiO2 (anatase powder was used as precursor to synthesize the TiO2 nanotubes. The TiO2 nanotubes were synthesized at 120 °C/20 hrs in an alkaline solution of NaOH. The photocatalytic study of the as-synthesized samples was conducted by analyzing the degradation of Rhodamine B (Rh B dye under UV light irradiation. Rh B is a toxic dye which is commonly used in textile industry. It is the one of the most common pollutants in the effluents and highly soluble in water. A comparison study was carried out in order to investigate the photocatalytic activity between the synthesized TiO2 nanotubes and the commercial TiO2 nanoparticles. Results show that the TiO2 nanotubes exhibits better photocatalytic activity in the degradation of Rh B as compared to the TiO2 nanoparticles.

  18. Hydrothermal crystallization and crystal growth of alpha-SiO /sub 2/ from rice husk silica ash in alkaline solution

    International Nuclear Information System (INIS)

    While pure and active amorphous silica was prepared from rice husk with 99.96% purity by acid leaching and pyrolysis. This silica could be crystallized into prismatic microcrystalline alpha-SiO/sub 2/ (77-88nm) under hydrothermal conditions (215 deg. C approx. equal to 20 atm. press) from alkaline solutions. The maximum yield of alpha-SiO/sub 2/ was obtained at 0.6 molar ratio of NaOH/SiO/sub 2/ which reduced to 0.2 in the presence of Al/sup 3+/ and /or B/sup 3+/ traces. This is due to formation of silicate crystals (considered to be a polymorph of SiO/sub 2/). It has been found that transition metal cations in the reaction media affects the degree of alpha-SiO/sub 2/ crystallization in the order: Fe/sup 3+/ > Ni/sup 2+/ > Cr/sup 3+/ > amorphous SiO/sub 2/ > Co/sup 2+/ > Cu/sup 2+/ > Mn/sup 2+/ > Zn/sup 2+/ > Zr/sup 2+/ > La/sup 3+/. Both mechanism of alpha-SiO/sub 2/ silicate growth and ions incorporation were discussed. (authors)

  19. Composition of Hydrothermal Vent Microbial Communities as Revealed by Analyses of Signature Lipids, Stable Carbon Isotopes and Aquificales Cultures

    Science.gov (United States)

    Jahnke, Linda L.; Eder, Wolfgang; Huber, Robert; Hinrichs, Kai-Uwe; Hayes, John M.; Cady, Sherry L.; DesMarais, David J.; Hope, Janet M.; Summons, Roger E.

    2001-01-01

    Extremely thermophilic microbial communities associated with the siliceous vent walls and outflow channel of Octopus Spring, Yellowstone National Park, have been examined for lipid biomarker and carbon isotopic signatures. These data were compared with that obtained from representatives of three Aquificales genera. Thermocrinis ruber, Thermocrinis sp. HI, Hydrogenobacter thermophilus, Aquifex pyrophilus and Aquifex aeolicus all contained phospholipids composed not only of the usual ester-linked fatty acids, but also ether-linked alkyl moieties. The fatty acids of all cultured organisms were dominated by very distinct pattern of n-C-20:1 and cy-C-21 compounds. The alkyl glycerol ethers were present primarily as C-18:0 monoethers with the exception of the Aquifex spp. in which dialkyl glycerol ethers with a boarder carbon-number distribution were also present. These Aquificales biomarker lipids were the major constituents in the lipid extracts of the Octopus Spring microbial samples. Two natural samples, a microbial biofilm growing in association with deposition of amorphous silica on the vent walls at 92 C, and the well-known "pink-streamer community" (PSC), siliceous filaments of a microbial consortia growing in the outflow channel at 87 C were analyzed. Both the biofilm and PSC samples contained mono- and dialkyl glycerol ethers with a prevalence of C-18 and C-20 alkyls. Phospholipid fatty acids were comprised of both the characteristic. Additional information is contained in the original extended abstract.

  20. Vallitalea pronyensis sp. nov., isolated from a marine alkaline hydrothermal chimney.

    Science.gov (United States)

    Ben Aissa, Fatma; Postec, Anne; Erauso, Gaël; Payri, Claude; Pelletier, Bernard; Hamdi, Moktar; Ollivier, Bernard; Fardeau, Marie-Laure

    2014-04-01

    A novel thermotolerant, anaerobic, Gram-stain-positive, spore-forming bacterium was isolated from a hydrothermal chimney in Prony Bay, New Caledonia. This strain, designated FatNI3(T), grew at 15-55 °C (optimum 30 °C) and at pH 5.8-8.9 (optimum 7.7). It was slightly halophilic, requiring at least 0.5 % NaCl for growth (optimum 2.5-3.0 %), and was able to grow at up to 6 % NaCl. Sulfate, thiosulfate, elemental sulfur, sulfite, nitrate and nitrite were not used as terminal electron acceptors. Growth of strain FatNI3(T) was inhibited in the presence of sulfite (2 mM) or nitrite (2 mM). Strain FatNI3(T) fermented cellobiose, glucose, mannose, maltose, sucrose, galactose, lactose, ribose, fructose, rhamnose, raffinose, xylose, yeast extract, peptone and biotrypticase. The main fermentation products from glucose metabolism were acetate, ethanol, H2 and CO2. The predominant cellular fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The main polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, and unknown glycolipids and phospholipids. The G+C content of the genomic DNA was 36.6 mol%. On the basis of phylogenetic and physiological properties, strain FatNI3(T) ( = DSM 25904 = JCM 18391) belonging to the phylum Firmicutes, class Clostridia, order Clostridiales, is proposed as the type strain of a novel species of the genus Vallitalea, for which the name Vallitalea pronyensis sp. nov. is proposed. PMID:24408522

  1. Experimental investigation of As, Sb and Cs behavior during olivine serpentinization in hydrothermal alkaline systems

    Science.gov (United States)

    Lafay, Romain; Montes-Hernandez, German; Janots, Emilie; Munoz, Manuel; Auzende, Anne Line; Gehin, Antoine; Chiriac, Rodica; Proux, Olivier

    2016-04-01

    While Fluid-Mobile Elements (FMEs) such as B, Sb, Li, As or Cs are particularly concentrated in serpentinites, data on FME fluid-serpentine partitioning, distribution, and sequestration mechanisms are missing. In the present experimental study, the behavior of Sb, As and Cs during San Carlos olivine serpentinization was investigated using accurate mineralogical, geochemical, and spectroscopic characterization. Static-batch experiments were conducted at 200 °C, under saturated vapor pressure (≈1.6 MPa), for initial olivine grain sizes of chemical measurements reveal that the As(V) is mainly adsorbed onto the serpentinization products, especially brucite. In contrast, mineralogical characterization combined with XAS spectroscopy reveal redox sensitivity for Sb sequestration within serpentine products, depending on the progress of the reaction. When serpentinization is attributed to a reduction of Sb(V) into Sb(III). This stage is characterized by the precipitation of Sb-Ni-rich phases and a lower bulk partitioning coefficient compared to that of the serpentine and brucite assemblage. Antimony reduction appears linked to water reduction accompanying the bulk iron oxidation, as half the initial Fe(II) is oxidized into Fe(III) and incorporated into the serpentine products once the reaction is over. The reduction of Sb implies a decrease of its solubility, but the type of secondary Sb-rich phases identified here might not be representative of natural systems where Sb concentrations are lower. These results bring new insights into the uptake of FME by sorption on serpentine products that may form in hydrothermal environments at low temperatures. FME sequestration here appears to be sensitive to various physicochemical parameters and more particularly to redox conditions that appear to play a preponderant role in the concentrations and mechanism of sequestration of redox-sensitive elements.

  2. 热液喷口生物体对成矿作用的影响%The effect of deep-sea hydrothermal vent organisms on the mineralization

    Institute of Scientific and Technical Information of China (English)

    尤继元; 周鼎武; 朱晓辉

    2011-01-01

    The discovery of deep-sea hydrothermal vents and associated biological group is one of the most important scientific achievements in global ocean science in the last 20 years.Around the Black Smoker,there is an extreme environment with high-temperature,high-pressure,darkness,hypoxia,sulfur,etc.,and there is a special biological community in the deep-sea.As the primary producers,thermophilic bacteria and archaea not only affect the deposition of minerals around the vents,but also directly or indirectly affect the dissolution,adsorption,extraction,and transformation of mineral elements,which causes the generation of Fe,Mn,non-ferrous metal oxide,sulfide,silicate and carbonate minerals and compounds in precipitation.It is important for the deep-sea mineral deposit enrichment.%深海热液喷口及相关生物群体的发现是近20年来全球海洋科学取得的最重要的科学成就之一。在海底黑烟囱周围高温、高压、黑暗、缺氧、含硫等极端环境中,生活着特殊的深海生物群落,它们的初级生产者嗜热细菌和古细菌在其自身的生活史中不仅影响了喷口周围矿物的沉积,而且直接和间接影响了矿物元素的溶解、吸附、萃取、转化,生成包括Fe,Mn及有色金属的氧化物、硫化物、硅酸盐及碳酸盐化合物的矿物与沉淀,对热液喷口矿床富集具有重要意义。

  3. Hydrothermal synthesis of Pb(Zr0.52Ti0.48)O3 powders at low temperature and low alkaline concentration

    Indian Academy of Sciences (India)

    Zhong-Cheng Qiu; Jian-Ping Zhou; Gangqiang Zhu; Peng Liu; Xiao-Bing Bian

    2009-04-01

    Pb(Zr0.52Ti0.48)O3 (PZT) powders were prepared by hydrothermal method. The effects of experimental parameters, including Pb/(Zr, Ti) ratio, alkaline concentration, reaction temperature and time on the product powders were studied in detail. Pure PZT powders were synthesized at suitable experimental conditions and Raman spectra confirmed the PZT with a perovskite-type structure. The homogeneous PZT powders with cubic-shaped morphology were formed at alkaline concentration of 1.2 M after reacting at 230°C for 2 h. The pure PZT powders obtained at low temperature and low alkaline concentration were attributed to precursors, TiCl4, with high activity and mineralizer NaOH with small cation radius.

  4. Hydrothermal mixing: Fuel for life in the deep-sea

    Science.gov (United States)

    Hentscher, M.; Bach, W.; Amend, J.; McCollom, T.

    2009-04-01

    Deep-sea hydrothermal vent systems show a wide range of fluid compositions and temperatures. They reach from highly alkaline and reducing, like the Lost City hydrothermal field, to acidic and reducing conditions, (e. g., the Logatchev hydrothermal field) to acidic and oxidizing conditions (e. g., island arc hosted systems). These apparently hostile vent systems are generally accompanied by high microbial activity forming the base of a food-web that often includes higher organisms like mussels, snails, or shrimp. The primary production is boosted by mixing of chemically reduced hydrothermal vent fluids with ambient seawater, which generates redox disequilibria that serve as energy source for chemolithoautotrophic microbial life. We used geochemical reaction path models to compute the affinities of catabolic (energy-harvesting) and anabolic (biosynthesis) reactions along trajectories of batch mixing between vent fluids and 2 °C seawater. Geochemical data of endmember hydrothermal fluids from 12 different vent fields (Lost City, Rainbow, Logatchev, TAG, EPR 21 °N, Manus Basin, Mariana Arc, etc.) were included in this reconnaissance study of the variability in metabolic energetics in global submarine vent systems. The results show a distinction between ultramafic-hosted and basalt-hosted hydrothermal systems. The highest energy yield for chemolithotrophic catabolism in ultramafic-hosted hydrothermal systems is reached at low temperature and under slightly aerobic to aerobic conditions. The dominant reactions, for example at Rainbow or Lost City, are the oxidation of H2, Fe2+ and methane. At temperatures >60 °C, anaerobic metabolic reactions, e. g., sulphate reduction and methanogenesis, become more profitable. In contrast, basalt-hosted systems, such as TAG and 21 °N EPR uniformly indicate H2S oxidation to be the catabolically dominant reaction over the entire microbial-relevant temperature range. Affinities were calculated for the formation of individual cellular

  5. Multiple I-Type Lysozymes in the Hydrothermal Vent Mussel Bathymodiolus azoricus and Their Role in Symbiotic Plasticity.

    Directory of Open Access Journals (Sweden)

    Camille Detree

    Full Text Available The aim of this study was first to identify lysozymes paralogs in the deep sea mussel Bathymodiolus azoricus then to measure their relative expression or activity in different tissue or conditions. B. azoricus is a bivalve that lives close to hydrothermal chimney in the Mid-Atlantic Ridge (MAR. They harbour in specialized gill cells two types of endosymbiont (gram-bacteria: sulphide oxidizing bacteria (SOX and methanotrophic bacteria (MOX. This association is thought to be ruled by specific mechanism or actors of regulation to deal with the presence of symbiont but these mechanisms are still poorly understood. Here, we focused on the implication of lysozyme, a bactericidal enzyme, in this endosymbiosis. The relative expression of Ba-lysozymes paralogs and the global anti-microbial activity, were measured in natural population (Lucky Strike--1700 m, Mid-Atlantic Ridge, and in in situ experimental conditions. B. azoricus individuals were moved away from the hydrothermal fluid to induce a loss of symbiont. Then after 6 days some mussels were brought back to the mussel bed to induce a re-acquisition of symbiotic bacteria. Results show the presence of 6 paralogs in B. azoricus. In absence of symbionts, 3 paralogs are up-regulated while others are not differentially expressed. Moreover the global activity of lysozyme is increasing with the loss of symbiont. All together these results suggest that lysozyme may play a crucial role in symbiont regulation.

  6. Multiple I-Type Lysozymes in the Hydrothermal Vent Mussel Bathymodiolus azoricus and Their Role in Symbiotic Plasticity.

    Science.gov (United States)

    Detree, Camille; Chabenat, Apolline; Lallier, François H; Satoh, Nori; Shoguchi, Eiichi; Tanguy, Arnaud; Mary, Jean

    2016-01-01

    The aim of this study was first to identify lysozymes paralogs in the deep sea mussel Bathymodiolus azoricus then to measure their relative expression or activity in different tissue or conditions. B. azoricus is a bivalve that lives close to hydrothermal chimney in the Mid-Atlantic Ridge (MAR). They harbour in specialized gill cells two types of endosymbiont (gram-bacteria): sulphide oxidizing bacteria (SOX) and methanotrophic bacteria (MOX). This association is thought to be ruled by specific mechanism or actors of regulation to deal with the presence of symbiont but these mechanisms are still poorly understood. Here, we focused on the implication of lysozyme, a bactericidal enzyme, in this endosymbiosis. The relative expression of Ba-lysozymes paralogs and the global anti-microbial activity, were measured in natural population (Lucky Strike--1700 m, Mid-Atlantic Ridge), and in in situ experimental conditions. B. azoricus individuals were moved away from the hydrothermal fluid to induce a loss of symbiont. Then after 6 days some mussels were brought back to the mussel bed to induce a re-acquisition of symbiotic bacteria. Results show the presence of 6 paralogs in B. azoricus. In absence of symbionts, 3 paralogs are up-regulated while others are not differentially expressed. Moreover the global activity of lysozyme is increasing with the loss of symbiont. All together these results suggest that lysozyme may play a crucial role in symbiont regulation. PMID:26882089

  7. Multiple I-Type Lysozymes in the Hydrothermal Vent Mussel Bathymodiolus azoricus and Their Role in Symbiotic Plasticity

    Science.gov (United States)

    Detree, Camille; Chabenat, Apolline; Lallier, François H.; Satoh, Nori; Shoguchi, Eiichi; Tanguy, Arnaud; Mary, Jean

    2016-01-01

    The aim of this study was first to identify lysozymes paralogs in the deep sea mussel Bathymodiolus azoricus then to measure their relative expression or activity in different tissue or conditions. B. azoricus is a bivalve that lives close to hydrothermal chimney in the Mid-Atlantic Ridge (MAR). They harbour in specialized gill cells two types of endosymbiont (gram—bacteria): sulphide oxidizing bacteria (SOX) and methanotrophic bacteria (MOX). This association is thought to be ruled by specific mechanism or actors of regulation to deal with the presence of symbiont but these mechanisms are still poorly understood. Here, we focused on the implication of lysozyme, a bactericidal enzyme, in this endosymbiosis. The relative expression of Ba-lysozymes paralogs and the global anti-microbial activity, were measured in natural population (Lucky Strike -1700m, Mid-Atlantic Ridge), and in in situ experimental conditions. B. azoricus individuals were moved away from the hydrothermal fluid to induce a loss of symbiont. Then after 6 days some mussels were brought back to the mussel bed to induce a re-acquisition of symbiotic bacteria. Results show the presence of 6 paralogs in B. azoricus. In absence of symbionts, 3 paralogs are up-regulated while others are not differentially expressed. Moreover the global activity of lysozyme is increasing with the loss of symbiont. All together these results suggest that lysozyme may play a crucial role in symbiont regulation. PMID:26882089

  8. Salinity, pressure and heavy-metal stress response of moderately halophilic bacteria isolated from hydrothermal-vent environments

    Science.gov (United States)

    Kaye, J. Z.; Baross, J. A.

    2002-12-01

    Moderately halophilic bacteria comprised 0.01-10% of the total microbial community in low-temperature hydrothermal emissions and in the overlying water column. The presence of these microorganisms was initially thought to be linked to brines that are produced by super-critical phase separation beneath deep-sea mid-ocean ridges. While there is conclusive evidence that these brines exist at extremely hot (>400°C) temperatures, it is difficult to construct geochemical and fluid-flow models which would delineate extensive subseafloor brine environments in mesophilic to hyperthermophilic temperature ranges. An alternative hypothesis is that halotolerance is actually induced by an environmental stress other than salt. Pressure and heavy metals are likely candidates. Diffuse flow environments at Axial Seamount and the Endeavour Segment of the Juan de Fuca Ridge and along the Southern East Pacific Rise are both elevated in concentrations of heavy metals and under moderate pressure (150-270 atm; higher beneath the seafloor). From these fluids we isolated numerous strains of moderately halophilic bacteria belonging to the genera Halomonas and Marinobacter. At ambient pressure, isolates grew between -1 and 40°C, with up to 25% NaCl, and with 2.0-3.0 mM cadmium. The isolates displayed widely varying pressure maxima and cell yields as a function of temperature and salinity. High pressure and salt (and heavy metals?) may independently induce a stress response that enables these bacteria to cope with all of these stresses. Also in progress are molecular-phylogenetic analyses of moderately halophilic bacterial populations from diffuse flow sites along the Juan de Fuca Ridge. We expect that many of the organisms detected using our novel primers will have been cultured. With the knowledge of their physiologies and how their diversity changes in relation to fluid chemistry, these data may shed light on the dynamic subseafloor hydrothermal system that supports them.

  9. Geochemical Controls on the Mobility of Cu and Fe in Hydrothermal Vent Fluids at Mid-Ocean Ridges: Experimental and Theoretical Constraints

    Science.gov (United States)

    Schaen, A. T.; Tutolo, B. M.; Seyfried, W. E.

    2012-12-01

    It has long been recognized that MOR hydrothermal vent fluids are characterized by variably high concentrations of dissolved transition metals. These metalliferous fluids play a role in the formation of seafloor massive sulfide deposits, serving as analogues for similar deposits on land, while also contributing to the flux of metals to seawater, with biogeochemical implications. Owing to the evolution of magmatic and tectonic processes associated with crustal formation at both fast and slow spreading ridges, chemical and physical conditions can change in space and time with corresponding changes in the solubility of Cu and Fe. Indeed, time series observations of hydrothermal vent fluids at EPR 9o N have provided unambiguous evidence of both diking and eruptive events with important implications for temperature and pressure changes affecting phase equilibria controls on mineral solubility. At the same time, recent advances in theoretical data have resulted in more robust thermodynamic models that can be used to calculate the effect of temperature and pressure, redox variability and dissolved chloride on metal mobility. However, fluid speciation calculations employing currently accepted Helgeson-Kirkham-Flowers (HKF) parameters for aqueous species result in Cu and Fe solubilities that differ significantly from constraints imposed by published experimental data and sampled MOR vent fluids. Consequently, new thermodynamic data is retrieved in this study from recent high P, T experimental data for Cu and Fe complexes and validated against new experiments to ensure accurate fluid speciation and trace metal solubility calculations. The addition of new experimental data to the thermodynamic data retrieval process strengthens predictions of geochemical interactions not only at the P and T of the experiments, but also over the entire range of applicability of the HKF model. For example, theoretical modeling of seawater salinity fluids (550 mmol/kg Cl) at 400 oC show

  10. Hydrothermal minerals

    Digital Repository Service at National Institute of Oceanography (India)

    Nath, B.N.

    ridge system, sulfide minerals crystallize from the hot water directly onto the volcanic rocks surrounding the hydrothermal vent leading to the formation of hollow, chimney like sulfide structure through which the hot water continues to flow... systems, which are in turn an integral component of global mid-ocean ridge system. Hydrothermal systems are composed of convection cells in the crust and upper mantle through which seawater freely circulates reacting with magmatic material, which...

  11. Implications of an ultramafic body in a basalt-dominated oceanic hydrothermal system on the vent fluid composition and on processes within sediments overlying a hydrothermal discharge zone: results of reactive-transport modeling

    Science.gov (United States)

    Alt-Epping, P.; Diamond, L. W.

    2009-04-01

    We use 2D reactive transport simulations to assess the hydraulic, thermal and chemical implications of an ultramafic body of lherzolitic composition within a basalt-dominated oceanic hydrothermal system. The simulations are fully coupled and hence account for the progressive serpentinization and the associated porosity/permeability reduction of the model lherzolite over time. We focus on the chemical fingerprints that reveal the presence of the ultramafic body at depth and that may be detected by direct seafloor exploration. These are the vent fluid composition and the porewater and mineral alteration within the rock column overlying a hydrothermal discharge zone. We compare ocean crust sections with and without sedimentary cover. Simulations suggest that the boundary between the basalt and the lherzolite constitutes a sharp reaction front. The type and distribution of alteration phases that form at the reaction front are a result of fluid flow across the basalt-lherzolite interface and thus are determined by the geometry and rate of hydrothermal fluid flow. Consequently, observations of the occurrence and extent of alteration phases, such as Fe-rich chlorite in the lherzolite or of rodingitization of the basalt, may be interpreted in terms of the reactive-transport model to reconstruct paleo-fluid flow in the permeable oceanic basement. The alteration of the lherzolite produces a fluid that is strongly reducing and depleted in silica. The most important chemical indicator of this rock-water interaction is an elevated H2 concentration. Under reducing (i.e. SO4-2 and CO2 free) conditions the enrichment in H2 is proportional to the extent of reaction between the fluid and the ultramafic rock. Under these conditions H2 behaves conservatively and the fluid remains enriched in H2 even though the concentration of all other major aqueous species is quickly buffered to new values when the fluid subsequently passes through basalt. This produces a vent fluid which is

  12. A Special Issue (Part-II): Mafic-ultramafic rocks and alkaline-carbonatitic magmatism and associated hydrothermal mineralization - dedication to Lia Nikolaevna Kogarko

    Science.gov (United States)

    Kogarko, Lia N.; Gwalani, Lalchand G.; Downes, Peter J.; Randive, Kirtikumar R.

    2015-10-01

    This is the second part of a two-volumespecial issue of Open Geoscience (formerly Central European Journal of Geosciences) that aims to be instrumental in providing an update of Mafic-Ultramafic Rocks and Alkaline- Carbonatitic Magmatism and Associated Hydrothermal Mineralization. Together, these two volumes provide a detailed and comprehensive coverage of the subjects that are relevant to the research work of P.Comin-Chiaramonti (Italy) and LiaN. Kogarko (Russia) towhomPart-I and Part- II have been respectively dedicated. To a significant extent, the development of advanced sampling technologies related to alkaline and carbonatitic magmatism by Lia N. Kogarko, has allowed geoscientists to measure and sample the deep crust of the planet not only for the exploration for the mineral deposits, but also to answer basic scientific questions about the origin and evolution of alkaline rocks (kimberlites, lamproites and related rocks associated with carbonatites). The papers presented in this Part-II of the special issue cover the petrology and geochemistry of the rocks collected from the surface and penetrated by drilling. Lia Kogarko proposed a new theory for the evolution of alkaline magmatism in the geological history of the Earth - that the appearance of alkaline magmatism at the Archaean-Proterozoic boundary (~2.5 - 2.7 Ga), and its growing intensity, was related to changes in the geodynamic regime of the Earth and oxidation of the mantle due to mantle-crust interaction.

  13. Behavioural study of two hydrothermal crustacean decapods: Mirocaris fortunata and Segonzacia mesatlantica, from the Lucky Strike vent field (Mid-Atlantic Ridge)

    Science.gov (United States)

    Matabos, M.; Cuvelier, D.; Brouard, J.; Shillito, B.; Ravaux, J.; Zbinden, M.; Barthelemy, D.; Sarradin, P. M.; Sarrazin, J.

    2015-11-01

    Identifying the factors driving community dynamics in hydrothermal vent communities, and in particular biological interactions, is challenged by our ability to make direct observations and the difficulty to conduct experiments in those remote ecosystems. As a result, we have very limited knowledge on species' behaviour and interactions in these communities and how they in turn influence community dynamics. Interactions such as competition or predation significantly affect community structure in vent communities, and video time-series have successfully been used to gain insights in biological interactions and species behaviour, including responses to short-term changes in temperature or feeding strategies. In this study, we combined in situ and ex situ approaches to characterise the behaviour and interactions among two key species encountered along the Mid-Atlantic Ridge (MAR): the shrimp Mirocaris fortunata and the crab Segonzacia mesatlantica. In situ, species small-scale distribution, interactions and behaviour were studied using the TEMPO observatory module deployed on the seafloor at the base of the active Eiffel Tower edifice in the Lucky Strike vent field as part of the EMSO-Açores MoMAR observatory. TEMPO sampled 2 min of video four times a day from July 2011 to April 2012. One week of observations per month was used for 'long-term' variations, and a full video data set was analysed for January 2012. In addition, observations of crab and shrimp individuals maintained for the first time under controlled conditions in atmospheric pressure (classic tank) and pressurised (AbyssBox) aquaria allowed better characterisation and description of the different types of behaviour and interactions observed in nature. While the identified in situ spatial distribution pattern was stable over the nine months, both species displayed a significant preference for mussel bed and anhydrite substrata, and preferentially occupied the area located directly in the fluid flow axis

  14. Deferrisoma paleochoriense sp. nov., a thermophilic, iron(III)-reducing bacterium from a shallow-water hydrothermal vent in the Mediterranean Sea

    Science.gov (United States)

    Perez-Rodriguez, Ileana M.; Rawls, Matthew; Coykendall, Dolly K.; Foustoukos, Dionysis I.

    2016-01-01

    A novel thermophilic, anaerobic, mixotrophic bacterium, designated strain MAG-PB1T, was isolated from a shallow-water hydrothermal vent system in Palaeochori Bay off the coast of the island of Milos, Greece. The cells were Gram-negative, rugose, short rods, approximately 1.0 μm long and 0.5 μm wide. Strain MAG-PB1T grew at 30–70 °C (optimum 60 °C), 0–50 g NaCl l− 1 (optimum 15–20 g l− 1) and pH 5.5–8.0 (optimum pH 6.0). Generation time under optimal conditions was 2.5 h. Optimal growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Fe(III), Mn(IV), arsenate and selenate were used as electron acceptors. Peptone, tryptone, Casamino acids, sucrose, yeast extract, d-fructose, α-d-glucose and ( − )-d-arabinose also served as electron donors. No growth occurred in the presence of lactate or formate. The G+C content of the genomic DNA was 66.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Deferrisoma camini, the first species of a recently described genus in the Deltaproteobacteria. Based on the 16S rRNA gene phylogenetic analysis and on physiological, biochemical and structural characteristics, the strain was found to represent a novel species, for which the name Deferrisoma palaeochoriense sp. nov. is proposed. The type strain is MAG-PB1T ( = JCM 30394T = DSM 29363T). 

  15. Deferrisoma paleochoriense sp. nov., a thermophilic, iron(III)-reducing bacterium from a shallow-water hydrothermal vent in the Mediterranean Sea

    Science.gov (United States)

    Perez-Rodriguez, Ileana; Rawls, Matthew; Coykendall, Dolly K.; Foustoukos, Dionysis I.

    2016-01-01

    A novel thermophilic, anaerobic, mixotrophic bacterium, designated strain MAG-PB1T, was isolated from a shallow-water hydrothermal vent system in Paleochori Bay off the coast of Milos Island, Greece. The cells were Gram-negative, rugose short rods approximately 1.0 μm in length and 0.5 μm in width. Strain MAG-PB1T grew between 30 and 70°C (optimum 60°C), 0 and 50 g NaCl l-1 (optimum 15-20 g l-1) and pH 5.5 and 8.0 (optimum pH 6.0). Generation time under optimal conditions was 2.5 hours. Optimal growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Fe(III), Mn(IV), arsenate and selenate were used as electron acceptors. Peptone, tryptone, Casamino acids, dextrose, sucrose, yeast extract, D-fructose, α-D-glucose and D-(-)-arabinose also served as electron donors. No growth occurred in the presence of lactate or formate. G + C content of the genomic DNA was 66.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Deferrisoma camini, a recently described genus in the Deltaproteobacteria. Based on the 16S rDNA phylogenetic analysis and on physiological, biochemical and structural characteristics, the strain was found to represent a novel species for which the name Deferrisoma paleochoriense sp. nov. is proposed. The type strain of Deferrisoma paleochoriense is MAG-PB1T (=JCM 30394 T; = DSM 29363T).

  16. Metalloid reducing bacteria isolated from deep ocean hydrothermal vents of the Juan de Fuca Ridge, Pseudoalteromonas telluritireducens sp. nov. and Pseudoalteromonas spiralis sp. nov.

    Science.gov (United States)

    Rathgeber, Christopher; Yurkova, Natalia; Stackebrandt, Erko; Schumann, Peter; Humphrey, Elaine; Beatty, J Thomas; Yurkov, Vladimir

    2006-11-01

    Five strains of Gram-negative, rod, curved rod and spiral-shaped bacteria were isolated from the vicinity of deep ocean hydrothermal vents along the Main Endeavour Segment of the Juan de Fuca Ridge in the Pacific Ocean. All strains showed remarkable resistance to high levels of toxic metalloid oxyanions, and were capable of reducing the oxyanions tellurite and selenite to their less toxic elemental forms. Phylogenetic analysis of four strains identified these isolates as close relatives of the genus Pseudoalteromonas within the class Gammaproteobacteria. Pseudoalteromonas agarivorans was the closest relative of strains Te-1-1 and Se-1-2-redT, with, respectively, 99.5 and 99.8% 16S rDNA sequence similarity. Strain Te-2-2T was most closely related to Pseudoalteromonas paragorgicola, with 99.8% 16S rDNA sequence similarity. The DNA G+C base composition was 39.6 to 41.8 mol%, in agreement with other members of the genus Pseudoalteromonas. However, the isolates showed important morphological and physiological differences from previously described species of this genus, with one group forming rod-shaped bacteria typical of Pseudoalteromonas and the other forming vibrioid- to spiral-shaped cells. Based on these differences, and on phylogenetic data, we propose the creation of the new species Pseudoalteromonas telluritireducens sp. nov., with strain Se-1-2-redT (DSMZ = 16098T = VKM B-2382T) as the type strain, and Pseudoalteromonas spiralis sp. nov., with strain Te-2-2T (DSMZ = 16099T = VKM B-2383T) as the type strain. PMID:17066332

  17. Deferrisoma palaeochoriense sp. nov., a thermophilic, iron(III)-reducing bacterium from a shallow-water hydrothermal vent in the Mediterranean Sea.

    Science.gov (United States)

    Pérez-Rodríguez, Ileana; Rawls, Matthew; Coykendall, D Katharine; Foustoukos, Dionysis I

    2016-02-01

    A novel thermophilic, anaerobic, mixotrophic bacterium, designated strain MAG-PB1T, was isolated from a shallow-water hydrothermal vent system in Palaeochori Bay off the coast of the island of Milos, Greece. The cells were Gram-negative, rugose, short rods, approximately 1.0 μm long and 0.5 μm wide. Strain MAG-PB1T grew at 30-70 °C (optimum 60 °C), 0-50 g NaCl l- 1 (optimum 15-20 g l- 1) and pH 5.5-8.0 (optimum pH 6.0). Generation time under optimal conditions was 2.5 h. Optimal growth occurred under chemolithoautotrophic conditions with H2 as the energy source and CO2 as the carbon source. Fe(III), Mn(IV), arsenate and selenate were used as electron acceptors. Peptone, tryptone, Casamino acids, sucrose, yeast extract, d-fructose, α-d-glucose and ( - )-d-arabinose also served as electron donors. No growth occurred in the presence of lactate or formate. The G+C content of the genomic DNA was 66.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that this organism is closely related to Deferrisoma camini, the first species of a recently described genus in the Deltaproteobacteria. Based on the 16S rRNA gene phylogenetic analysis and on physiological, biochemical and structural characteristics, the strain was found to represent a novel species, for which the name Deferrisoma palaeochoriense sp. nov. is proposed. The type strain is MAG-PB1T ( = JCM 30394T = DSM 29363T). PMID:26610851

  18. Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability

    Directory of Open Access Journals (Sweden)

    Holder Thomas

    2013-01-01

    Full Text Available Abstract Background Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias, which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation. Reviewer This article was reviewed by Sándor Pongor, L. Aravind and Anthony M. Poole.

  19. U and Th Concentration and Isotopic Composition of Hydrothermal Fluids at the Lost City Hydrothermal Field

    Science.gov (United States)

    Ludwig, K. A.; Shen, C.; Cheng, H.; Edwards, R.; Kelley, D. S.; Butterfield, D. A.

    2006-12-01

    Uranium and Th concentration and isotopic composition of hydrothermal fluids at the Lost City Hydrothermal Field (LCHF) were determined using multi-collector inductively coupled plasma mass spectrometry (MC-ICP- MS). The LCHF is an off-axis, serpentinite-hosted hydrothermal system located at 30°N near the Mid- Atlantic Ridge. Carbonate chimneys reaching 60 m in height vent alkaline (pH~10), calcium-rich fluids at 40- 91°C and the towers are home to dense microbial communities. Vent fluid and seawater U and Th concentration and isotopic composition data provide critical information for constraining U-Th chimney ages. The increased sensitivity (1-2%) of MC-ICP-MS combined with an Aridus nebulization system allows the precise measurement of small quantities of sample (~150 ml) with low concentrations (Thorium concentrations of fluids are close to deep seawater values. U and Th isotopic compositions are reported at the permil level. These data may provide new insights into the role of serpentinite-hosted hydrothermal systems in the budgets of U and Th in the ocean. Techniques presented in this study may be applied to other hydrothermal and seep environments.

  20. 集成深拖与AUV对洋中脊热液喷口的联合探测%Joint exploration by Deep Tow and AUV for hydrothermal vent on ocean ridge

    Institute of Scientific and Technical Information of China (English)

    周建平; 陶春辉; 金翔龙; 李怀明; 邓显明; 顾春华; 何拥华

    2011-01-01

    Detection of hydrothermal vents is an international hot spot. There are various detection technology and methods for the detection. Deep Tow and Automatic Underwater Vehicle (AUV) are important and popular technology, which is de-scribed in this paper with a focus on the combination characteristics of integration Deep Tow. According to the demand of exploration on hydrothermal vents and the advantages of both methods, a new method is presented, which is a joint exploration by both techniques. The effectiveness of the new method is discussed using an example of an actual exploration on East Pa-cific Rise. This new method can reduce the blindness of dive of AUV, by using the survey result of Deep Tow instead of the first phase of normal AUV survey, so the exploration time will be shortened. Two important factors, correcting the actual site of Deep Tow and the effect of current and topography on deployment to the hydrothermal vent, have been identified on the spot detection and data analysis. This study provides an important guidance for future exploration on hydrothermal vents.%热液喷口探测是目前国际上的热点,探测技术方法也多种多样.阐述了集成深拖与自治水下机器人(Autonomous Underwater Vehicle,AUV)探测技术,重点介绍了集成深拖的组合特性.针对热液喷口的探测需求,根据集成深拖与AUV两种方法的优点,提出两种技术方法的联合探测.以一个实际探测的区块为例,讨论了两种方法联合探测的实效性.指出该技术方法可更快速地探测热液的异常范围,同时可降低AUV下潜的盲目性,也可以减少AUV的下潜次数,节约了海上调查时间,提高探测效率.最后指出了现场探测与数据分析需进行深拖位置校正和关注底流及地形对热液羽状流空间分布的影响两个重要问题,为未来的热液探测提供了指导作用.

  1. Characterization of Microbial Communities Associated With Deep-Sea Hydrothermal Vent Animals of the East Pacific Rise and the Galápagos Rift

    Science.gov (United States)

    Ward, N.; Page, S.; Heidelberg, J.; Eisen, J. A.; Fraser, C. M.

    2002-12-01

    The composition of microbial communities associated with deep-sea hydrothermal vent animals is of interest because of the key role of bacterial symbionts in driving the chemosynthetic food chain of the vent system, and also because bacterial biofilms attached to animal exterior surfaces may play a part in settlement of larval forms. Sequence analysis of 16S ribosomal RNA (rRNA) genes from such communities provides a snapshot of community structure, as this gene is present in all Bacteria and Archaea, and a useful phylogenetic marker for both cultivated microbial species, and uncultivated species such as many of those found in the deep-sea environment. Specimens of giant tube worms (Riftia pachyptila), mussels (Bathymodiolus thermophilus), and clams (Calyptogena magnifica) were collected during the 2002 R/V Atlantis research cruises to the East Pacific Rise (9N) and Galápagos Rift. Microbial biofilms attached to the exterior surfaces of individual animals were sampled, as were tissues known to harbor chemosynthetic bacterial endosymbionts. Genomic DNA was extracted from the samples using a commercially available kit, and 16S rRNA genes amplified from the mixed bacterial communities using the polymerase chain reaction (PCR) and oligonucleotide primers targeting conserved terminal regions of the 16S rRNA gene. The PCR products obtained were cloned into a plasmid vector and the recombinant plasmids transformed into cells of Escherichia coli. Individual cloned 16S rRNA genes were sequenced at the 5' end of the gene (the most phylogenetically informative region in most taxa) and the sequence data compared to publicly available gene sequence databases, to allow a preliminary assignment of clones to taxonomic groups within the Bacteria and Archaea, and to determine the overall composition and phylogenetic diversity of the animal-associated microbial communities. Analysis of Riftia pachyptila exterior biofilm samples revealed the presence of members of the delta and

  2. Evolution of the sulfide-binding function within the globin multigenic family of the deep-sea hydrothermal vent tubeworm Riftia pachyptila.

    Science.gov (United States)

    Bailly, Xavier; Jollivet, Didier; Vanin, Stephano; Deutsch, Jean; Zal, Franck; Lallier, François; Toulmond, André

    2002-09-01

    The giant extracellular hexagonal bilayer hemoglobin (HBL-Hb) of the deep-sea hydrothermal vent tube worm Riftia pachyptila is able to transport simultaneously O(2) and H(2)S in the blood from the gills to a specific organ: the trophosome that harbors sulfide-oxidizing endosymbionts. This vascular HBL-Hb is made of 144 globins from which four globin types (A1, A2, B1, and B2) coevolve. The H(2)S is bound at a specific location (not on the heme site) onto two of these globin types. In order to understand how such a function emerged and evolved in vestimentiferans and other related annelids, six partial cDNAs corresponding to the six globins known to compose the multigenic family of R. pachyptila have been identified and sequenced. These partial sequences (ca. 120 amino acids, i.e., 80% of the entire protein) were used to reconstruct molecular phylogenies in order to trace duplication events that have led to the family organization of these globins and to locate the position of the free cysteine residues known to bind H(2)S. From these sequences, only two free cysteine residues have been found to occur, at positions Cys + 1 (i.e., 1 a.a. from the well-conserved distal histidine) and Cys + 11 (i.e., 11 a.a. from the same histidine) in globins B2 and A2, respectively. These two positions are well conserved in annelids, vestimentiferans, and pogonophorans, which live in sulfidic environments. The structural comparison of the hydrophobic environment that surrounds these cysteine residues (the sulfide-binding domain) using hydrophobic cluster analysis plots, together with the cysteine positions in paralogous strains, suggests that the sulfide-binding function might have emerged before the annelid radiation in order to detoxify this toxic compound. Moreover, globin evolutionary rates are highly different between paralogous strains. This suggests that either the two globin subfamilies involved in the sulfide-binding function (A2 and B2) have evolved under strong directional

  3. FTIR study of the photocatalytic degradation of gaseous benzene over UV-irradiated TiO2 nanoballs synthesized by hydrothermal treatment in alkaline solution

    International Nuclear Information System (INIS)

    In this study, photocatalysts of TiO2 nanoballs were obtained via a hydrothermal treating of commercial P25 in alkaline solution, and then characterized with SEM, XRD, BET and surface photovoltage spectroscopy techniques. The UV-assisted photodegradation of gaseous benzene over P25 and the prepared TiO2 nanoballs was monitored by an in situ infrared technique. The results demonstrated that the prepared TiO2 nanoballs in anatase form were more active than commercial P25 in photocatalytic oxidation of gaseous benzene. The promoted activity of the hydrothermal-treated TiO2 is attributed to the increasing specific surface area and larger band gap induced by the reduced crystallite size. The spectra of FTIR indicated that weakly adsorbed phenol was formed as the reaction progress. Hydroxyl groups on the surface of TiO2 nanoballs are able to react with photo-produced phenol, which is then retained on the catalyst surface leading to the progressive deactivation of the catalyst in the gas-solid system.

  4. Combined pretreatment using alkaline hydrothermal and ball milling to enhance enzymatic hydrolysis of oil palm mesocarp fiber.

    Science.gov (United States)

    Zakaria, Mohd Rafein; Hirata, Satoshi; Hassan, Mohd Ali

    2014-10-01

    Hydrothermal pretreatment of oil palm mesocarp fiber was conducted in tube reactor at treatment severity ranges of log Ro = 3.66-4.83 and partial removal of hemicellulose with migration of lignin was obtained. Concerning maximal recovery of glucose and xylose, 1.5% NaOH was impregnated in the system and subsequent ball milling treatment was employed to improve the conversion yield. The effects of combined hydrothermal and ball milling pretreatments were evaluated by chemical composition changes by using FT-IR, WAXD and morphological alterations by SEM. The successful of pretreatments were assessed by the degree of enzymatic digestibility of treated samples. The highest xylose and glucose yields obtained were 63.2% and 97.3% respectively at cellulase loadings of 10 FPU/g-substrate which is the highest conversion from OPMF ever reported. PMID:25058299

  5. Ultra-diffuse hydrothermal venting supports Fe-oxidizing bacteria and massive umber deposition at 5000 m off Hawaii

    OpenAIRE

    Edwards, Katrina J.; Glazer, B T; Rouxel, O J; W. Bach; Emerson, D; Davis, R. E.; Toner, B M; Chan, C S; Tebo, B M; Staudigel, H; Moyer, C L

    2011-01-01

    A novel hydrothermal field has been discovered at the base of Lōihi Seamount, Hawaii, at 5000 mbsl. Geochemical analyses demonstrate that ‘FeMO Deep', while only 0.2 °C above ambient seawater temperature, derives from a distal, ultra-diffuse hydrothermal source. FeMO Deep is expressed as regional seafloor seepage of gelatinous iron- and silica-rich deposits, pooling between and over basalt pillows, in places over a meter thick. The system is capped by mm to cm thick hydrothermally derived iro...

  6. 台湾龟山岛东南部海域白色热泉特征分析%Characteristic analysis of White hydrothermal vent in the southeastern Kueishantao Islet off Taiwan Island

    Institute of Scientific and Technical Information of China (English)

    韩沉花; 叶瑛; 潘依雯; 秦华伟

    2014-01-01

    海底热液活动及其周边环境的调查研究是海洋科学领域的热点之一。海底热液喷口环境的物理化学参数,尤其是浅水型热液,会受到潮汐变化的影响。台湾龟山岛靠近冲绳海槽南端分布有多处浅水型热液喷口(<30 m )。2010年5月,浙江大学、杭州电子科技大学和台湾中山大学对该海域实施了联合考察,利用电化学传感器获得了低温热液喷口(24.83414°N ,121.96191°E )连续87 h的温度和pH现场观测数据。基于Morlet复小波变换方法,分析了浅水型热液喷口的温度和pH现场观测时间序列的变化周期,并通过与当地潮汐记录数据作比较,获得了其与潮汐信号之间的潜在关系,证实了潮汐变化是海底白色热泉喷口物理化学参数现场观测最主要的影响因素。温度和pH现场观测数据经Morlet复小波变换后的小波系数分别存在规律的12.9 h和12.5 h变化周期,并且温度的小波系数在准半日周期尺度与潮汐高度呈负相关变化,相关系数为-0.89;p H的小波系数情况相反,相关系数为0.76。%Investigation into seafloor hydrothermal activity and its surroundings has been one key point of studying ocean sciences .Physical and chemical parameters obtained from the hydrothermal environments would be influ -enced by the tide ,especially from the shallow hydrothermal vent .It is distributing many hydrothermal vents on the less than 30 meter shallow seafloor of Kueishantao Islet ,Northeast offshore Taiwan Island ,close to the south-ern Okinawa Trough .Zhejiang University ,Hangzhou Dianzi University ,and Taiwan Sun Yat-sen University to-gether carried a joint investigation into seafloor hydrothermal near Kueishan island sea area and obtained continuous temperature and pH field observation data of a low-temperature hydrothermal vent (24.83414° N ,121.961 91° E) for about 87 hours .Based on Morlet complex wavelet transform method ,after

  7. 海底热液口温度场高精度声学测量方法研究%Study on high precision acoustic measurement techniques for determining temperature fields around seafloor hydrothermal vents

    Institute of Scientific and Technical Information of China (English)

    蔡勇; 潘宏; 周艳; 樊炜; 付现桥

    2012-01-01

    This paper presents the basic principle of the acoustic method used for temperature field measurement of seafloor hydrothermal vents, which includes total least-squares algorithm and parabolic interpolation technique. Experiment research was performed to exactly rebuild the temperature fields around the hot springs on the floor of Lake Qiezishan, Yunnan. The accuracy of time of flight estimation has been improved based on the parabolic interpolation technique. At the same time, through comparing the two temperature fields obtained before and after parabolic interpolation, the max absolute error, the max relative error and the root mean square error are given. Experiment result shows that the total least-squares algorithm and the parabolic interpolation technique can be applied to temperature field measurement detection around seafloor hydrothermal vents, and good reconstruction accuracy is achieved.%介绍了海底热液口温度场原位声学测量的基本原理、总体最小二乘重建方法和抛物插值算法.在云南省龙陵县茄子山水库进行湖底试验研究,基于总体最小二乘重建方法准确地还原出湖底热泉的温度场原位分布;将抛物插值算法应用于声波飞渡时间延时估计,并对抛物插值前后重建出的温度场进行最大绝对误差、最大相对误差和均方根误差分析.实验表明:总体最小二乘重建方法和抛物插值算法可用于海底热液口温度场的原位测量,且重建精度比较高.

  8. The geophysical characteristics of hydrothermal plumes at the Logatchev vent field, Mid-Atlantic Ridge%大西洋中脊Logatchev热液区的地球物理场研究

    Institute of Scientific and Technical Information of China (English)

    唐勇; 和转; 吴招才; 黎明碧

    2012-01-01

    利用“大洋一号”首次环球科考获得的地球物理资料,通过对多波束地形和地磁异常的解译,对大西洋Logatchev热液区的地球物理场特征进行了研究,研究结果表明Logatchev热液喷口位于裂谷东侧的滑塌体上,处于南北地磁正负高值异常中心之间的缓冲带上,新发现的磁异常区与热液喷口区具有相似的特征,推测为未来的热液矿区.区内南西西-北东东和北-南向断裂不仅控制了基底深度和隆坳结构,也控制了超基性岩和热源磁性层的分布,因此它的形成与交叉切割的拆离断层、超基性岩、海洋核杂岩和重力滑塌有关.%Magnetic and multibeam data were collected from DAYANG No. 1 first world cruise at Mid Atlantic Ridge. The interpretation of magnetic anomalies and bathymetric data shows the geophysical characteristics of hy-drothermal plumes at the Logatchev vent field. Logatchev vent site is situated on the eastern inner flank of the rift valley and between the high value of positive and negative anomalies. NWW-SEE and NS trending fault not only control basement depth and upwelling-sag,but also dose the distribution of ultramafic rock and heat source. It is suggest that the Logatchev hydrothermal field is associated with ultramafic-hosted, off-axis location with detachment faulting, oceanic core complex, abundant gravitational landslides.

  9. Microbe-related precipitation of iron and silica in the Edmond deep-sea hydrothermal vent field on the Central Indian Ridge

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    Microbial mats, several millimeters thick and brown-yellow to white in color, were collected in hollow inside of chimney structure from Edmond hydrothermal field on the Central Indian Ridge. Microbes with shapes of rod and helical stalk-like filaments were observed in the microbial mats, and are commonly characterized by their cells completely encrusted by thick mineralized layers, made up of large amounts of amorphous silica and minor amounts of iron oxides. Transmission Electron Microscope observation has demonstrated that the acicular Fe-bearing matter was not only heterogenously distributed on the surface of the cell wall, but also deposited in the inside of cell, suggesting that bio-precipitation of Fe had occurred both on the surface and in the interior of cell. Microbial silicification was also commonly found in the mats. Silica usually precipitated homogeneously on the surface of the microbes and forms micro-laminated layers, which might be controlled by the inorganic process of precipitation in hydrothermal environment. The biomineralization phenomenon in the microbial mats showed that the precipitation of Fe and Si was closely related to microbes in hydrothermal environment. Considering that hydrothermal activities provided required chemical elements for mineralization, it is suggested that this biomineralization process also might be driven by hydrothermal activities at the sea floor to some extent.

  10. Comment on 'Consequences of phase separation on the distribution of hydrothermal fluids at ASHES vent field, axial volcano, Juan de Fuca ridge' by Christopher G. Fox

    Science.gov (United States)

    Scholl, M. A.; Ingebritsen, S. E.; Essaid, H. I.

    1993-02-01

    Fox (1990), in order to explain observations during the Axial Seamount Hydrothermal Emissions Study (ASHES), proposed a conceptual model for a two-phase subsea hydrothermal system in which steam controlled flow patterns by blocking liquid flow. An attempt is made here to demonstrate with a very general model that relative permeability contrasts by themselves do not cause spatial isolation of phases in steam/liquid water systems and that density segregation, independent of relative permeability effects, should not be ruled out as an explanation for the observations at the ASHES site. Fox replies that density segregation is probably not the only mechanism at work.

  11. THE DIVERSITY AND DISTRIBUTION OF ARCHAEA IN GUAYMAS DEEP-SEA HYDROTHERMAL VENT%瓜伊马斯深海热液口古菌分布及多样性研究

    Institute of Scientific and Technical Information of China (English)

    鲁书林; 何莹; 王风平

    2013-01-01

    Due to the dramatic gradients of temperature (up to above 400℃ ) ,pH and chemicals, deep-sea hydrothermal vents have been the focus for studies of the origin and evolution of life on earth and the subseafloor hydrothermal living system is ideal for exploring the origin and evolution of deep-sea life. In this study, the diversity of archaea was investigated at high temperature sediments and chimney from Guaymas hydrothermal vent using cultured-independent technique. Environmental total DNA were directly extracted and used for the quantification and construction of archaea 16S rRNA gene libraries. A total of 79 and 80 clean sequences were obtained from the sediment and chimney samples. Quantitative PCR analysis revealed that the amounts of archaea present in the sediment and chimney were about 1.47×109 copies/g ( wet weight) and 5. 29 × 108 copies/g ( wet weight), respectively. All the clone libraries had the coverage C value more than 80%, the archaea diversity of sediment sample was higher than the chimney. The compositions of archaea community were different between two samples, yet most of archaea belonged to the thermophilic/hyperthermophilic microorganism because of the high temperature environment. In the sediments, most of archaea came from Crenarchaeota domain and the two most dominant groups were the Miscellaneous Crenarchaeota Group ( MCG,43. 04% )and Hot Water Crenarchaeota Group Ⅰ ( HWCG Ⅰ , 20. 25% ) confirmed by archaeal 16S rRNA gene components. The remaining sequences from the sediment sample were related to members of Thermoprotei ( 7. 59%) , Korarchaeota ( 1. 26%) , Methanosarcinales (1. 26%) , Marine Benthic Group D/Deep Hydrothermal Vent Euryarchaeota ( MBGD/DHVE 1, 8. 86%) , DHVE 9 ( 5. 06%) , Miscellaneous Euryarchaeota Group ( MEG, 2. 53%). While the Euryarchaeota domain dominated the chimney sample, in which Thermococcales (47.5%), Marine Benthic Group D ( MBGD )/Deep Hydrothermal Vent Euryarchaeota 1 ( DHVE 1) ( MBGD/DHVE 1

  12. Post-capture immune gene expression studies in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus acclimatized to atmospheric pressure

    Digital Repository Service at National Institute of Oceanography (India)

    Barros, I.; Divya, B.; Martins, I.; Vandeperre, F.; Santos, R.S.; Bettencourt, R.

    ) following the manufacturer's specifi- cations and re-suspended in nuclease-free, DEPC-treated water. Total RNA quality preparations and concentrations were assessed by the A260/280 and A260/230 spectrophotometric ratios using the NanoVue spectrophotometer... in all cDNA synthesis. The cDNA concentration was measured using the NanoVue spectrophotometer (A260/280 and A260/230 spectrophotometric ratios). 2.5. Real-time PCR (qPCR) analyses Based on sequences retrieved from the DeepSeaVent database (http...

  13. Sclerite formation in the hydrothermal-vent “scaly-foot” gastropod — possible control of iron sulfide biomineralization by the animal

    OpenAIRE

    Suzuki, Yohey; Kopp, Robert E.; Kogure, Toshihiro; Suga, Akinobu; Takai, Ken; Tsuchida, Shinji; Ozaki, Noriaki; Endo, Kazuyoshi; Hashimoto, Jun; Kato, Yasuhiro; Mizota, Chitoshi; Hirata, Takafumi; CHIBA, Hitoshi; Nealson, Kenneth H; Horikoshi, Koki

    2006-01-01

    A gastropod from a deep-sea hydrothermal field at the Rodriguez triple junction, Indian Ocean, has scale-shaped structures, called sclerites, mineralized with iron sulfides on its foot. No other organisms are known to produce a skeleton consisting of iron sulfides. To investigate whether iron sulfide mineralization is mediated by the gastropod for the function of the sclerites, we performed a detailed physical and chemical characterization. Nanostructural characterization of the iron sulfide ...

  14. The research on hydrothermal vent detection with the multiparameter chemical sensor%基于多参数化学传感器的海底热液探测方法研究

    Institute of Scientific and Technical Information of China (English)

    潘依雯; 武光海; 秦华伟; 韩沉花; 叶瑛

    2012-01-01

    On the basis of chemical anomalies between hydrothermal plumes and surrounding seawater a means of detecting hydrothermal activity is proposed. Therefore, a multiparameter chemical sensor integrating three types of electrochemical electrodes, including Eh, Ag/Ag2S and pH electrodes, is designed to be applied in deep-sea measurements during DY115 -20 cruise by Dayang No. 1 research vessel. It is proved by the measuring results that the multiparameter chemical sensor can help to detect the chemical a-nomalies of the plumes. The electrodes exhibit good sensitivity, stability and long lifetime in the multiple applications during six months. Among the 60 measurements in both East Pacific Ridge (EPR) and Southwest Indian Ridge (SWIR) , the integrated sensor records 55 groups of valid data, in which 29 records indicate potential active hydrothermal vents, which proves an effective tool in hydrothermal activity detection.%基于热液及其羽状流的化学异常提出了一种海底热液的化学式探测方法.根据自行开发的Eh.Ag/Ag2S和pH三种化学电极,设计了多参数化学传感器系统.通过海试证明多参数化学传感器可灵敏地检测出由热液异常引起的化学量变化,并在长达半年的多次使用中表现出其稳定性好、寿命长的特点.在东太平洋中脊(EPR)赤道区域和西南印度洋中脊(SWIR)的60条测线中,获得有效测线55条,其中在29条测线上发现明显或可能的热液异常.研究表明,应用多参数化学传感器探测进行海底热液化学异常,是一种有效的方法.

  15. 声学法深海热液速度场测量重建算法研究%Reconstruction algorithm for acoustic measurement of velocity field of deep-sea hydrothermal vents

    Institute of Scientific and Technical Information of China (English)

    白燕; 毛洁; 樊炜; 潘华辰; 刘云峰

    2011-01-01

    介绍了利用声学法测量深海热液速度场的基本原理.利用往返飞渡时间差与流场速度的关系,应用反问题求解技术重建测量区域的速度场.应用最小二乘法对两种典型的热液口速度场模型进行了仿真重建,分析了换能器数量、实验测量误差以及换能器的布放对重建结果的影响,并对重建结果进行了流量分析.仿真重建的绝对误差、相对误差以及流量分析结果表明,最小二乘法具有较高重建精度,增加声学换能器及细化网格可提高重建精度,换能器对称分布时具有最优重建结果.最后对声学法深海热液温度场速度场测量系统进行了介绍和说明.深海热液速度场的测量重建可为热液热通量的测量奠定基础.%The basic theory of acoustic velocity field measurement in deep-sea hydrothermal vents was introduced.The time of flight (TOF) of acoustic signals through hydrothermal vents was dependent on temperature and velocity.The velocity field was reconstructed by inverse problem solving techniques based on the relationship between the round-trip TOF difference and velocity of flow. The reconstruction of velocity field using the least square method was presented. At the same time, flow flux of reconstruction results was analyzed. The results show that the least square method has good accuracy. Increase of the number of acoustic transducers and mesh density can improve the reconstruction accuracy effectively.

  16. First Comparative Analysis of the Community Structures and Carbon Metabolic Pathways of the Bacteria Associated with Alvinocaris longirostris in a Hydrothermal Vent of Okinawa Trough

    Science.gov (United States)

    Sun, Qing-lei; Zeng, Zhi-gang; Chen, Shuai; Sun, Li

    2016-01-01

    Alvinocaris longirostris is a species of shrimp existing in the hydrothermal fields of Okinawa Trough. To date the structure and function of the microbial community associated with A. longirostris are essentially unknown. In this study, by employment of the techniques of high through-put sequencing and clone library construction and analysis, we compared for the first time the community structures and metabolic profiles of microbes associated with the gill and gut of A. longirostris in a hydrothermal field of Okinawa Trough. Fourteen phyla were detected in the gill and gut communities, of which 11 phyla were shared by both tissues. Proteobacteria made up a substantial proportion in both tissues, while Firmicutes was abundant only in gut. Although gill and gut communities were similar in bacterial diversities, the bacterial community structures in these two tissues were significantly different. Further, we discovered for the first time the existence in the gill and gut communities of A. longirostris the genes (cbbM and aclB) encoding the key enzymes of Calvin-Benson-Bassham (CBB) cycle and the reductive tricarboxylic acid (rTCA) cycle, and that both cbbM and aclB were significantly more abundant in gill than in gut. Taken together, these results provide the first evidence that at least two carbon fixation pathways are present in both the gill and the gut communities of A. longirostris, and that the communities in different tissues likely differ in autotrophic productivity. PMID:27111851

  17. Fluid composition of the sediment-influenced Loki's Castle vent field at the ultra-slow spreading Arctic Mid-Ocean Ridge

    Science.gov (United States)

    Baumberger, Tamara; Früh-Green, Gretchen L.; Thorseth, Ingunn H.; Lilley, Marvin D.; Hamelin, Cédric; Bernasconi, Stefano M.; Okland, Ingeborg E.; Pedersen, Rolf B.

    2016-08-01

    The hydrothermal vent field Loki's Castle is located in the Mohns-Knipovich bend (73°N) of the ultraslow spreading Arctic Mid-Ocean Ridge (AMOR) close to the Bear Island sediment fan. The hydrothermal field is venting up to 320° C hot black smoker fluids near the summit of an axial volcanic ridge. Even though the active chimneys have grown on a basaltic ridge, geochemical fluid data show a strong sedimentary influence into the hydrothermal circulation at Loki's Castle. Compelling evidence for a sediment input is given by high alkalinity, high concentrations of NH4+, H2, CH4, C2+ hydrocarbons as well as low Mn and Fe contents. The low δ13C values of CO2 and CH4 and the thermogenic isotopic pattern of the C2+ hydrocarbons in the high-temperature vent fluids clearly point to thermal degradation of sedimentary organic matter and illustrate diminution of the natural carbon sequestration in sediments by hydrothermal circulation. Thus, carbon-release to the hydrosphere in Arctic regions is especially relevant in areas where the active Arctic Mid-Ocean Ridge system is in contact with the organic matter rich detrital sediment fans.

  18. Modeling lithosphere hydration processes at slow spreading ridges: What are the parameters controlling the formation of low temperature ultramafic hydrothermal vents?

    Science.gov (United States)

    Gouze, P.; Lods, G.; Godard, M.; Andreani, M.

    2008-12-01

    The numerous ultramafic-hosted hydrothermal systems discovered during the last ten years emphasized the importance of peridotite alteration (serpentinization) as means of chemical exchange between the mantle and the ocean at slow- and ultra-slow spreading ridges. Hydration of the cooling ultramafic lithosphere results from the thermal retraction of the poly-mineral mantle assemblage and/or faulting of stretched lithosphere; it induces in return strong changes in the permeability field. A better understanding of this highly complex system is essential for constraining mass and energy transfers at ridge axis. In order to characterize the roles of these various parameters as well as their feedback effects and to explore different hydro-geothermal initial and boundary conditions, we carried out a series of a two-dimensional flow/reaction simulations. In the model, thermal convection flow is coupled with serpentinization, which is described by the thermo- kinetic-controlled reaction of the hydration of olivine and orthopyroxene (assumed to be pure pure forsterite and enstatite respectively). The changes in porosity and permeability associated with (i) thermal retraction of the solid while cooling and (ii) serpentinization processes are computed including energy production and water consumption during reaction. These highly coupled processes generate a non-linear evolution of the flow regimes, inducing (transient) focalized fluid outputs (events) triggered by the thermal convection. The comparison between the results of numerical simulations and field observations (distribution of the events, outlet fluid composition and distribution of the serpentine at depth) is used to discuss the model parameters and determine which ones control the hydrodynamic processes in natural hydrothermal systems.

  19. Formaldehyde as a carbon and electron shuttle between autotroph and heterotroph populations in acidic hydrothermal vents of Norris Geyser Basin, Yellowstone National Park.

    Science.gov (United States)

    Moran, James J; Whitmore, Laura M; Isern, Nancy G; Romine, Margaret F; Riha, Krystin M; Inskeep, William P; Kreuzer, Helen W

    2016-05-01

    The Norris Geyser Basin in Yellowstone National Park contains a large number of hydrothermal systems, which host microbial populations supported by primary productivity associated with a suite of chemolithotrophic metabolisms. We demonstrate that Metallosphaera yellowstonensis MK1, a facultative autotrophic archaeon isolated from a hyperthermal acidic hydrous ferric oxide (HFO) spring in Norris Geyser Basin, excretes formaldehyde during autotrophic growth. To determine the fate of formaldehyde in this low organic carbon environment, we incubated native microbial mat (containing M. yellowstonensis) from a HFO spring with (13)C-formaldehyde. Isotopic analysis of incubation-derived CO2 and biomass showed that formaldehyde was both oxidized and assimilated by members of the community. Autotrophy, formaldehyde oxidation, and formaldehyde assimilation displayed different sensitivities to chemical inhibitors, suggesting that distinct sub-populations in the mat selectively perform these functions. Our results demonstrate that electrons originally resulting from iron oxidation can energetically fuel autotrophic carbon fixation and associated formaldehyde excretion, and that formaldehyde is both oxidized and assimilated by different organisms within the native microbial community. Thus, formaldehyde can effectively act as a carbon and electron shuttle connecting the autotrophic, iron oxidizing members with associated heterotrophic members in the HFO community. PMID:26995682

  20. Formaldehyde as a carbon and electron shuttle between autotroph and heterotroph populations in acidic hydrothermal vents of Norris Geyser Basin, Yellowstone National Park

    Energy Technology Data Exchange (ETDEWEB)

    Moran, James J.; Whitmore, Laura M.; Isern, Nancy G.; Romine, Margaret F.; Riha, Krystin M.; Inskeep, William P.; Kreuzer, Helen W.

    2016-03-19

    The Norris Geyser Basin in Yellowstone National Park contains a large number of hydrothermal systems, which host microbial populations supported by primary productivity associated with a suite of chemolithotrophic metabolisms. We demonstrate that Metallosphaera yellowstonesis MK1, a facultative autotrophic archaeon isolated from a hyperthermal acidic hydrous ferric oxide (HFO) spring in Norris Geyser Basin, excretes formaldehyde during autotrophic growth. To determine the fate of formaldehyde in this low organic carbon environment, we incubated native microbial mat (containing M. yellowstonensis) from a HFO spring with 13C-formaldehyde. Isotopic analysis of incubation-derived CO2 and biomass showed that formaldehyde was both oxidized and assimilated by members of the community. Autotrophy, formaldehyde oxidation, and formaldehyde assimilation displayed different sensitivities to chemical inhibitors, suggesting that distinct sub-populations in the mat selectively perform these functions. Our results demonstrate that electrons originally resulting from iron oxidation can energetically fuel autotrophic carbon fixation and associated formaldehyde excretion, and that formaldehyde is both oxidized and assimilated by different organisms within the native microbial community. Thus, formaldehyde can effectively act as a carbon and electron shuttle connecting the autotrophic, iron oxidizing members with associated heterotrophic members in the HFO community.

  1. The vent microbiome: patterns and drivers

    Science.gov (United States)

    Pachiadaki, M.

    2015-12-01

    Microbial processes within deep-sea hydrothermal vents affect the global biogeochemical cycles. Still, there are significant gaps in our understanding of the microbiology and the biogeochemistry of deep-sea hydrothermal systems. Vents differ in temperature, host rock composition and fluid chemistry; factors that are hypothesized to shape the distribution of the microbial communities, their metabolic capabilities and their activities. Using large-scale single cell genomics, we obtained insights into the genomic content of several linkages of a diffuse flow vent. The genomes show high metabolic versatility. Sulfur oxidation appears to be predominant but there is the potential of using a variety of e- donors and acceptors to obtain energy. To further assess the ecological importance of the vent auto- and heterotrophs, the global biogeography of the analyzed lineages will be investigated by fragment recruitment of metagenomes produced from the same site as well as other hydrothermal systems. Metatranscriptomic and metaproteomic data will be integrated to examine the expression of the predominant metabolic pathways and thus the main energy sources driving chemoautotrophic production. The comparative analysis of the key players and associated pathways among various vent sites that differ in physicochemical characteristics is anticipated to decipher the patterns and drivers of the global dispersion and the local diversification of the vent microbiome.

  2. Cloning and identification of chitin binding proteins of tubeworm,Ridgeia piscesae, from hydrothermal vent%热液区管状蠕虫几丁质结合蛋白的克隆表达及功能鉴定

    Institute of Scientific and Technical Information of China (English)

    闫鑫富; 阮灵伟

    2012-01-01

    Hydrothermal vent tube worm is the one of the most typical hydrothermal species. In previous study,we constructed cDNA library of tube worm(Ridgeia piscesae) and sequencing result showed that it contains 23 chitin-binding proteins( CBPs). With high transcription level and diversity,each protein contains 1 ~3 different types of chitin binding domain ( s). In view of the important role in chitin degradation and the special habitats of the tube worms , we further studied the functional characterization of 4 typical chitin-binding proteins. After analyzed, the sequences of signal peptides were cut off and the fragments encoding chitin-binding proteins were amplified by PCR, inserted into pIZ-FLAG Vector and successfully expressed in Hi5 cells. In the chitin affinity assay,the results demonstrated that four FLAG-CBPs can bind to a-chitin respectively. Meanwhile, the supernatant of cell lysate including the expressed product FLAG-CBPs was able to enhance the activity of chitinase in the process of degrading a-chitin and β-chitin respectively. All the results suggest that four CBPs may act as a-chitin binding proteins and may enhance the activity of chitinase. In-depth study will be taken to verify other biological functions of chitin binding proteins and optimize their ability of catalyzation.%本文应用PCR技术对四种管状蠕虫几丁质结合蛋白进行了扩增,进而连接到pIZ-FLAG构建了重组表达载体,并在昆虫细胞中实现了几丁质结合蛋白的异源重组表达.我们对几丁质结合蛋白的功能进行了初步研究,亲和活性实验结果表明这四种几丁质结合蛋白对α-chitin具有亲和活性,并且可以辅助几丁质酶,对降解α-chitin和β-chitin均有不同程度的促进作用,且作用效果明显.结果表明,这四种几丁质结合蛋白均为α型,且可以促进几丁质酶的活性,对降解几丁质多糖具有巨大的应用潜力和应用价值.

  3. Survey of genome size in 10 invertebrates from hydrothermal vent and cold seep%热液和冷泉10种无脊椎动物基因组大小测定及比较

    Institute of Scientific and Technical Information of China (English)

    郑平; 王敏晓; 李超伦; 孙松

    2016-01-01

    Knowledge of genome size is an important prerequisite for the development of many genomic resources. To better understand the evolution of genome size and their roles in environmental adaption,it is necessary to col-lect data covering a broad taxonomic base,especially from particular ecosystems.With high disturbance and unique environmental features,hydrothermal vent and cold seep offer a typical model to investigate the variation of genome size under extreme environments.Here we measured 10 deep-sea invertebrates from hydrothermal vent and cold seep by flow cytometry.The C-value ranged from 0.87 pg to 12.28 pg,and the crustaceans have larger genomes. Then we compared the three common species (Bathymodiolus platifrons ,Shinkaia crosnieri and Alvinocaris lon-girostris )in both environments.Though with high level of intraspecific variations,no significant differences were revealed between two populations.We also compared the obtained genome sizes to their corresponding taxonomic relatives (at family or order level).No evidence of genome size expansion or reduction were found in invertebrates from chemosynthetic environments.In our study,there are no clear trends of the genome size variations under the extreme deep-sea ecological conditions.%基因组大小(或称 C 值)作为生物单倍体细胞中全套染色体的 DNA 总量,在一定程度上是恒定的,因而 C 值可以作为生物物种的一个特定参数。深海热液和冷泉为更好地理解 C 值与不同环境之间的关系提供了一个特征性的模型。本文采用流式细胞术,测定了来自热液和冷泉环境中的10种深海无脊椎动物的 C 值,其分布范围从0.87 pg 到12.28 pg,其中,相比于软体动物和多毛类,甲壳生物基因组大小及变异均较大。对比热液和冷泉两个群落中共有种(深海偏顶蛤 Bathymodiolus plati-frons 、柯氏潜铠虾 Shinkaia crosnieri 以及长角阿尔文虾Alvinocaris longirostris

  4. Fabrication of free-standing NiCo2O4 nanoarrays via a facile modified hydrothermal synthesis method and their applications for lithium ion batteries and high-rate alkaline batteries

    International Nuclear Information System (INIS)

    Graphical abstract: Hydrothermal-synthesized NiCo2O4 nanoflake arrays exhibit porous structure and high capacity as well as good cycling life for lithium ion batteries and alkaline batteries. - Highlights: • Self-supported NiCo2O4 nanoflake arrays are prepared by a hydrothermal method. • NiCo2O4 nanoflake arrays show high capacity and good cycling life. • Porous nanoflake arrays structure is favorable for fast ion/electron transfer. - Abstract: Self-supported NiCo2O4 nanoflake arrays on nickel foam are prepared by a facile hydrothermal method. The obtained NiCo2O4 nanoflakes with thicknesses of ∼25 nm grow vertically to the nickel foam substrate and form an interconnected porous network with pore diameters of 50–500 nm. As anode material of LIBs, the NiCo2O4 nanoflake arrays show a high initial coulombic efficiency of 76%, as well as good cycling stability with a capacity of 880 mAh g−1 at 0.5 A g−1, and 523 mAh g−1 at 1.5 A g−1 after 50 cycles. As the cathode of alkaline batteries, a high capacity of 95 mAh g−1 is achieved at 2 A g−1 and 94% retention is maintained after 10,000 cycles. The superior electrochemical performance is mainly due to the unique nanoflake arrays structure with large surface area and shorter diffusion length for mass and charge transport

  5. Fabrication of free-standing NiCo{sub 2}O{sub 4} nanoarrays via a facile modified hydrothermal synthesis method and their applications for lithium ion batteries and high-rate alkaline batteries

    Energy Technology Data Exchange (ETDEWEB)

    Zheng, Qingyun, E-mail: hizhengqingyun@126.com; Zhang, Xiangyang; Shen, Youming

    2015-03-15

    Graphical abstract: Hydrothermal-synthesized NiCo{sub 2}O{sub 4} nanoflake arrays exhibit porous structure and high capacity as well as good cycling life for lithium ion batteries and alkaline batteries. - Highlights: • Self-supported NiCo{sub 2}O{sub 4} nanoflake arrays are prepared by a hydrothermal method. • NiCo{sub 2}O{sub 4} nanoflake arrays show high capacity and good cycling life. • Porous nanoflake arrays structure is favorable for fast ion/electron transfer. - Abstract: Self-supported NiCo{sub 2}O{sub 4} nanoflake arrays on nickel foam are prepared by a facile hydrothermal method. The obtained NiCo{sub 2}O{sub 4} nanoflakes with thicknesses of ∼25 nm grow vertically to the nickel foam substrate and form an interconnected porous network with pore diameters of 50–500 nm. As anode material of LIBs, the NiCo{sub 2}O{sub 4} nanoflake arrays show a high initial coulombic efficiency of 76%, as well as good cycling stability with a capacity of 880 mAh g{sup −1} at 0.5 A g{sup −1}, and 523 mAh g{sup −1} at 1.5 A g{sup −1} after 50 cycles. As the cathode of alkaline batteries, a high capacity of 95 mAh g{sup −1} is achieved at 2 A g{sup −1} and 94% retention is maintained after 10,000 cycles. The superior electrochemical performance is mainly due to the unique nanoflake arrays structure with large surface area and shorter diffusion length for mass and charge transport.

  6. Interactions Between Serpentinization, Hydrothermal Activity and Microbial Community at the Lost City Hydrothermal Field

    Science.gov (United States)

    Delacour, A.; Frueh-Green, G. L.; Bernasconi, S. M.; Schaeffer, P.; Frank, M.; Gutjahr, M.; Kelley, D. S.

    2008-12-01

    Seafloor investigations of slow- and ultraslow-spreading ridges have reported many occurrences of exposed mantle peridotites and gabbroic rocks on the ocean floor. Along the Mid-Atlantic Ridge, these uplifted portions of oceanic crust host high-temperature black smoker-type hydrothermal systems (e.g., Rainbow, Logatchev, Saldanha), and the more distinct low-temperature Lost City Hydrothermal Field (LCHF). Built on a southern terrace of the Atlantis Massif, the LCHF is composed of carbonate-brucite chimneys that vent alkaline and low-temperature (40-90°C) hydrothermal fluids. These fluids are related to serpentinization of mantle peridotites, which together with minor gabbroic intrusions form the basement of the LCHF. Long-lived hydrothermal activity at Lost City led to extensive seawater-rock interaction in the basement rocks, as indicated by seawater-like Sr- and mantle to unradiogenic Nd-isotope compositions of the serpentinites. These high fluid fluxes in the southern part of the massif influenced the conditions of serpentinization and have obliterated the early chemical signatures in the serpentinites, especially those of carbon and sulfur. Compared to reducing conditions commonly formed during the first stages of serpentinization, serpentinization at Lost City is characterized by relatively oxidizing conditions resulting in a predominance of magnetite, the mobilization/dissolution and oxidation of igneous sulfides to secondary pyrite, and the incorporation of seawater sulfate, all leading to high bulk-rock S-isotope compositions. The Lost City hydrothermal fluids contain high concentrations in methane, hydrogen, and low-molecular weight hydrocarbons considered as being produced abiotically. In contrast, organic compounds in the serpentinites are dominated by the occurrences of isoprenoids (pristane, phytane, and squalane), polycyclic compounds (hopanes and steranes), and higher abundances of C16 to C20 n-alkanes indicative of a marine organic input. We

  7. Effects of Alkali and Alkaline Earth Cocations on the Activity and Hydrothermal Stability of Cu/SSZ-13 NH3-SCR Catalysts

    Energy Technology Data Exchange (ETDEWEB)

    Gao, Feng; Wang, Yilin; Washton, Nancy M.; Kollar, Marton; Szanyi, Janos; Peden, Charles HF

    2015-10-13

    Using a three-step aqueous solution ion-exchange method, cocation modified Cu/SSZ-13 SCR catalysts were synthesized. These catalysts, in both fresh and hydrothermally aged forms, were characterized with several methods including temperature-programmed reduction by H2 (H2-TPR), temperature-programmed desorption of NH3 (NH3-TPD), and 27Al solid-state nuclear magnetic resonance (NMR) and diffuse reflectance Infrared Fourier Transform (DRIFT) spectroscopies. Their catalytic performance was probed using steady-state standard NH3-SCR. Characterization results indicate that cocations weaken interactions between Cu-ions and the CHA framework making them more readily reducible. By removing a portion of Brønsted acid sites, cocations also help to mitigate hydrolysis of the zeolite catalysts during hydrothermal aging as evidenced from 27Al NMR. Reaction tests show that certain cocations, especially Li+ and Na+, promote low-temperature SCR rates while others show much less pronounced effects. In terms of applications, our results indicate that introducing cocations can be a viable strategy to improve both low- and high-temperature performance of Cu/SSZ-13 SCR catalysts.

  8. Global Fluorine Flux Associated with Submarine Hydrothermal Fluids

    Science.gov (United States)

    Kagoshima, T.; Sano, Y.

    2015-12-01

    We estimated a fluorine flux associated with hydrothermal fluid emission at mid-ocean ridges (MOR) based on vent fluid chemistry and MORB vesicle compositions. Multiplication of fluorine concentrations in submarine hydrothermal fluids and the vent fluid flux at MOR may give us an estimate of fluorine flux at MOR. A worldwide vent chemistry research [1] suggested that submarine vent fluids are depleted in fluorine (Elsevier, London). [2] Coogan & Dosso (2012) EPSL 323-324, 92-101. [3] Kagoshima et al. (2014) Japan Geoscience Union Meeting 2014 Abstract #SGC56-P01. [4] Fischer (2008) Geochem. J. 42, 21-38.

  9. Hydrothermal alkaline stability of bentonite barrier by concrete interstitial wastes; Alteracion alcalina hidrotermal de la barrera de bentonita por aguas intersticiales de cementos

    Energy Technology Data Exchange (ETDEWEB)

    Leguey Jimenez, S.; Cuevas Rodriguez, J.; Ramirez Martin, S.; Vigil de la villa Mencia, R.; Martin Barca, M. [Universidad Autonoma de Madrid, Madrid (Spain)

    2002-07-01

    At present, the main source of High Level radioactive Waste (HLW) is the electrical energy production during all the steps of developing. In almost all the countries with nuclear programs, the option for the final management of HLW is the Deep Geological Repository (DGR) based on the concept of multi barrier. According to this concept, the waste is isolated from biosphere by the interposition of confinement barriers. Two of the engineering barriers in the Spanish design of DGR in granitic rock are compacted bentonite and concrete. The bentonite barrier is the backfilling and sealing material for the repository gallery, because of its mechanical and physico-chemical properties. The main qualities of concrete as a component of a multi barrier system are its low permeability, mechanical resistance and chemical properties. With regard to chemical composition of concrete, the alkaline nature of cement pore water lowers the solubility of many radioactive elements. However, structural transformation in smectite, dissolution or precipitation of minerals and, consequently, changes in the bentonite properties could occurs in the alkaline conditions generated by the cement degradation. The main objective of the present work is to evaluate the effect of concrete in the stability of Spanish reference bentonite (La Serrata of Nijar, Almeria, Spain) in conditions similar to those estimated in a DGR in granitic rock. Because of the main role of bentonite barrier in the global performance of the repository, the present study is essential to guarantee its security. (Author)

  10. A data processing method for MAPR hydrothermal plume tur-bidity data and its application in the Precious Stone Mountain hydrothermal field

    Institute of Scientific and Technical Information of China (English)

    CHEN Sheng; TAO Chunhui; LI Huaiming; CHEN Yongshun; ZHOU Jianping; WU Tao

    2014-01-01

    Hydrothermal plume is an important constituent of seabed hydrothermal circulation and is also one of the characteristics of active hydrothermal vents. Portable Miniature Autonomous Plume Recorders (MAPR) attached to a towed deep-sea instrument was used to search for hydrothermal plumes and hydrothermal vents. We introduced the basic principle of MAPR based on deep towing technology to detect plumes, then analyzed the factors affecting the quality of the MAPR data and presented a data correction method for MAPR, including instrument location correction, noise reduction processing, system error elimination and seawater background reduction. Finally we applied the method to analyze MAPR data obtained during the Chinese DY115-21 cruise on R/VDayang Iin the “Precious Stone Mountain” hydrothermal field on the Gala-pagos Microplate. The results provided a better understanding of the distribution of the hydrothermal activ-ity in this field, indicating the presence of a new hydrothermal vent.

  11. Shallow submarine hydrothermal activity with significant contribution of magmatic water producing talc chimneys in the Wakamiko Crater of Kagoshima Bay, southern Kyushu, Japan

    Science.gov (United States)

    Yamanaka, Toshiro; Maeto, Kotaro; Akashi, Hironori; Ishibashi, Jun-Ichiro; Miyoshi, Youko; Okamura, Kei; Noguchi, Takuroh; Kuwahara, Yoshihiro; Toki, Tomohiro; Tsunogai, Urumu; Ura, Tamaki; Nakatani, Takeshi; Maki, Toshihiro; Kubokawa, Kaoru; Chiba, Hitoshi

    2013-05-01

    Active hydrothermal venting from shallow seafloor (200-m depth) with talc chimneys has been discovered at the Wakamiko Crater floor in the Aira Caldera, southern Kyushu, Japan. The major chemical composition of the fluids suggests that the fluids are supplied from a single reservoir. The fluid is characterized by a low chloride concentration, low δD value, and a high δ18O value, suggesting that the endmember hydrothermal fluid is a mixture of seawater and andesitic water and possibly contribution of meteoric water and/or phase separation. Such noticeable magmatic input may be supported by high helium isotopic ratio (6.77 RA) of fumarolic gas discharging from the crater. Silica and alkaline geothermometers indicate that the fluid-rock interaction in the reservoir occurs in the temperature range of 230 to 250 °C. The high alkalinity and high ammonium and dissolved organic matter concentrations in the fluid indicate interaction of the fluid with organic matter in sedimentary layers. At least three hydrothermal vents have been observed in the crater. Two of these have similar cone-shaped chimneys. The chimneys have a unique mineralogy and consist dominantly of talc (kerolite and hydrated talc) with lesser amounts of carbonate (dolomite and magnesite), anhydrite, amorphous silica, and stibnite. The precipitation temperature estimated from δ18O values of talc was almost consistent with the observed fluid temperature. Geochemical modeling calculations also support the formation of talc and carbonate upon mixing of the endmember hydrothermal fluid with seawater and suggest that the talc chimneys are currently growing from venting fluid.

  12. Geochemistry driven trends in microbial diversity and function across a temperature transect of a shallow water hydrothermal system off Milos (Greece)

    Science.gov (United States)

    Bühring, Solveig I.; Amend, Jan P.; Gómez Sáez, Gonzalo V.; Häusler, Stefan; Hinrichs, Kai-Uwe; Pichler, Thomas; Pop Ristova, Petra; Price, Roy E.; Santi, Ioulia; Sollich, Miriam

    2014-05-01

    The shallow water hydrothermal vents off Milos Island, Greece, discharge hot, slightly acidic, reduced fluids into colder, slightly alkaline, oxygenated seawater. Gradients in temperature, pH, and geochemistry are established as the two fluids mix, leading to the formation of various microbial microniches. In contrast to deep-sea hydrothermal systems, the availability of sun light allows for a combination of photo- and chemotrophic carbon fixation. Despite the comparably easy accessibility of shallow water hydrothermal systems, little is known about their microbial diversity and functioning. We present data from a shallow hydrothermal system off Milos Island, one of the most hydrothermally active regions in the Mediterranean Sea. The physico-chemical changes from ambient seafloor to hydrothermal area were investigated and documented by in situ microsensor profiling of temperature, pH, total reduced sulfur and dissolved oxygen alongside porewater geochemistry. The spatial microbial diversity was determined using a combination of gene- and lipid-based approaches, whereas microbial functioning was assessed by stable isotope probing experiments targeting lipid biomarkers. In situ microprofiles indicated an extreme environment with steep gradients, offering a variety of microniches for metabolically diverse microbial communities. We sampled a transect along a hydrothermal patch, following an increase in sediment surface temperature from background to 90°C, including five sampling points up to 20 cm sediment depth. Investigation of the bacterial diversity using ARISA revealed differences in the community structure along the geochemical gradients, with the least similarity between the ambient and highly hydrothermally impacted sites. Furthermore, using multivariate statistical analyses it was shown that variations in the community structure could be attributed to differences in the sediment geochemistry and especially the sulfide content, and only indirectly to shifts in

  13. Bathymodiolus growth dynamics in relation to environmental fluctuations in vent habitats

    Science.gov (United States)

    Nedoncelle, K.; Lartaud, F.; Contreira Pereira, L.; Yücel, M.; Thurnherr, A. M.; Mullineaux, L.; Le Bris, N.

    2015-12-01

    The deep-sea mussel Bathymodiolus thermophilus is a dominant species in the East Pacific Rise (EPR) hydrothermal vent fields. On the EPR volcanically unstable area, this late colonizer reaches high biomass within 4-5 years on new habitats created by lava flows. The environmental conditions and growth rates characterizing the reestablishment of B. thermophilus populations are however largely unknown, leaving unconstrained the role of this foundation species in the ecosystem dynamics. A typical example from the vent field at 9°50'N that was affected by the last massive eruption was the Bio-9 hydrothermal vent site. Here, six years later, a large mussel population had reestablished. The von Bertalanffy growth model estimates the oldest B. thermophilus specimens to be 1.3 year-old in March 2012, consistent with the observation of scarce juveniles among tubeworms in 2010. Younger cohorts were also observed in 2012 but the low number of individuals, relatively to older cohorts, suggests limited survival or growth of new recruits at this site, that could reflect unsuitable habitat conditions. To further explore this asumption, we investigated the relationships between mussel growth dynamics and habitat properties. The approach combined sclerochronology analyses of daily shell growth with continuous habitat monitoring for two mussel assemblages; one from the Bio-9 new settlement and a second from the V-vent site unreached by the lava flow. At both vent sites, semi-diurnal fluctuations of abiotic conditions were recorded using sensors deployed in the mussel bed over 5 to 10 days. These data depict steep transitions from well oxygenated to oxygen-depleted conditions and from alkaline to acidic pH, combined with intermittent sulfide exposure. These semi-diurnal fluctuations exhibited marked changes in amplitude over time, exposing mussels to distinct regimes of abiotic constraints. The V-vent samples allowed growth patterns to be examined at the scale of individual life and

  14. Chemical Structure of Bioethanol Lignin by Low-Temperature Alkaline Catalytic Hydrothermal Treatment%水热法碱活化生物质乙醇木质素的化学结构变化

    Institute of Scientific and Technical Information of China (English)

    刘晓欢; 张明明; 王基夫; 许玉芝; 王春鹏; 储富祥

    2013-01-01

    为了提高生物质乙醇木质素的反应活性,采用水热法在四种不同碱性条件下对生物质乙醇木质素进行催化活化处理。运用傅里叶变换红外光谱(FTIR)、核磁共振氢谱(1 H-NMR)、凝胶色谱(GPC)和有机元素分析手段研究了生物质乙醇木质素被四种碱(NaO H ,KO H ,K2 CO3和Na2 CO3)催化活化前后木质素的化学结构以及组分变化。FTIR结果表明生物质乙醇木质素碱经处理后,木质素的酚羟基特征吸收峰1375 cm -1都有明显增大趋势,醚键振动吸收峰1116 cm -1减弱,1597和1511 cm-1处苯环骨架振动吸收峰强度变化很小;1 H-NMR分析结果表明酚羟基含量都有增大趋势,增加顺序为:KOH > NaOH > K2CO3>Na2 CO3,其中KOH处理后的木质素酚羟基含量增加量为原木素的170%。这由于离子半径大的钾离子更容易与木质素β-O-4醚键上的氧形成加和物,进而发生醚键断裂反应,生成新的酚结构衍生物。G PC 表明生物质乙醇木质素碱处理后分子量分布向低分子区域扩展,数均和重均分子量减小。元素分析结果显示木质素经过水热反应处理后,C含量都有所增加,而H和O含量则降低了,表明木质素经水热反应处理过程中有脱羧基作用,同时蛋白质的含量也有所降低,提高了木质素的纯度。这都有利于直接将木质素用于制备酚醛树脂胶黏剂。%In order to improve the reaction activity of bioethanol lignin ,we investigated the activation of bioethanol lignin by a hydrothermal treatment method .Catalytic hydrothermal treatment of bioethanol lignin was performed at 180 ℃ for 3 h in the presence of alkaline solutions (NaOH ,Na2CO3 ,KOH and K2CO3 ) ,the change in bioethanol lignin structures was studied com-paratively by FTIR ,1 H NMR ,GPC and elemental analysis .FTIR spectra showed that after alkali hydrothermal treatment ,the band at 1 375 cm-1 attributed to the

  15. Hydrothermal plumes and processes in Indian Ocean

    OpenAIRE

    Sands, Carla Marie

    2006-01-01

    The predicted cycling of the whole ocean through hydrothermal plumes is comparable to the mixing time of the oceans (few thousand years). Hence, understanding hydrothermal plume processes is crucial if their impact on the global geochemical cycles of elements is to be assessed. One of the most important processes that has been demonstrated to modify the gross chemical flux from venting to the oceans is the oxidative precipitation of dissolved Fe (II). It has been hypothesised t...

  16. 24 CFR 3280.611 - Vents and venting.

    Science.gov (United States)

    2010-04-01

    ... DEVELOPMENT MANUFACTURED HOME CONSTRUCTION AND SAFETY STANDARDS Plumbing Systems § 3280.611 Vents and venting... required by structural conditions, vent piping may offset below the rim of the fixture at the maximum...

  17. Geologic evolution of the Lost City Hydrothermal Field

    Science.gov (United States)

    Denny, Alden R.; Kelley, Deborah S.; Früh-Green, Gretchen L.

    2016-02-01

    The Lost City Hydrothermal Field (LCHF) is a novel serpentinite-hosted vent field located on the Atlantis Massif southern wall. Results of 2 m resolution bathymetry, side scan, and video and still imagery, integrated with direct submersible observations provide the first high-resolution geologic map of the LCHF. These data form the foundation for an evolutionary model for the vent system over the past >120,000 years. The field is located on a down-dropped bench 70 m below the summit of the massif. The bench is capped by breccia and pelagic carbonate deposits underlain by variably deformed and altered serpentinite and gabbroic rocks. Hydrothermal activity is focused at the 60 m tall, 100 m across, massive carbonate edifice "Poseidon," which is venting 91°C fluid. Hydrothermal activity declines south and west of the Poseidon complex and dies off completely at distances greater than 200 m. East of Poseidon, the most recent stage of hydrothermal flow is characterized by egress of diffuse fluids from narrow fissures within a low-angle, anastomosing mylonite zone. South of the area of current hydrothermal activity, there is evidence of two discrete previously unrecognized relict fields. Active venting sites defined by carbonate-filled fissures that cut the carbonate cap rock at the summit of the massif mark the present-day northernmost extent of venting. These spatial relationships reflect multiple stages of field development, the northward migration of venting over time, and the likely development of a nascent field at the massif summit.

  18. Seawater bicarbonate removal during hydrothermal circulation

    Science.gov (United States)

    Proskurowski, G. K.; Seewald, J.; Sylva, S. P.; Reeves, E.; Lilley, M. D.

    2013-12-01

    High temperature fluids sampled at hydrothermal vents represent a complex alteration product of water-rock reactions on a multi-component mixture of source fluids. Sources to high-temperature hydrothermal samples include the 'original' seawater present in the recharge limb of circulation, magmatically influenced fluids added at depth as well as any seawater entrained during sampling. High-temperature hydrothermal fluids are typically enriched in magmatic volatiles, with CO2 the dominant species, characterized by concentrations of 10's-100's of mmol/kg (1, 2). Typically, the high concentration of CO2 relative to background seawater bicarbonate concentrations (~2.3 mmol/kg) obscures a full analysis of the fate of seawater bicarbonate during high-temperature hydrothermal circulation. Here we present data from a suite of samples collected over the past 15 years from high-temperature hydrothermal vents at 9N, Endeavour, Lau Basin, and the MAR that have endmember CO2 concentrations less than 10 mmol/kg. Using stable and radiocarbon isotope measurements these samples provide a unique opportunity to examine the balance between 'original' seawater bicarbonate and CO2 added from magmatic sources. Multiple lines of evidence from multiple hydrothermal settings consistently points to the removal of ~80% of the 'original' 2.3 mmol/kg seawater bicarbonate. Assuming that this removal occurs in the low-temperature, 'recharge' limb of hydrothermal circulation, this removal process is widely occurring and has important contributions to the global carbon cycle over geologic time. 1. Lilley MD, Butterfield DA, Lupton JE, & Olson EJ (2003) Magmatic events can produce rapid changes in hydrothermal vent chemistry. Nature 422(6934):878-881. 2. Seewald J, Cruse A, & Saccocia P (2003) Aqueous volatiles in hydrothermal fluids from the Main Endeavour Field, northern Juan de Fuca Ridge: temporal variability following earthquake activity. Earth and Planetary Science Letters 216(4):575-590.

  19. Hydrothermal Conditions and the Origin of Cellular Life.

    Science.gov (United States)

    Deamer, David W; Georgiou, Christos D

    2015-12-01

    The conditions and properties of hydrothermal vents and hydrothermal fields are compared in terms of their ability to support processes related to the origin of life. The two sites can be considered as alternative hypotheses, and from this comparison we propose a series of experimental tests to distinguish between them, focusing on those that involve concentration of solutes, self-assembly of membranous compartments, and synthesis of polymers. Key Word: Hydrothermal systems. PMID:26684507

  20. Understanding vented gas explosions

    Energy Technology Data Exchange (ETDEWEB)

    Lautkaski, R. [VTT Energy, Espoo (Finland). Energy Systems

    1997-12-31

    The report is an introduction to vented gas explosions for nonspecialists, particularly designers of plants for flammable gases and liquids. The phenomena leading to pressure generation in vented gas explosions in empty and congested rooms are reviewed. The four peak model of vented gas explosions is presented with simple methods to predict the values of the individual peaks. Experimental data on the external explosion of dust and gas explosions is discussed. The empirical equation relating the internal and external peak pressures in vented dust explosions is shown to be valid for gas explosion tests in 30 m{sup 3} and 550 m{sup 3} chambers. However, the difficulty of predicting the internal peak pressure in large chambers remains. Methods of explosion relief panel design and principles of vent and equipment layout to reduce explosion overpressures are reviewed. (orig.) 65 refs.

  1. Chemical environments of submarine hydrothermal systems

    Science.gov (United States)

    Shock, Everett L.

    1992-01-01

    Perhaps because black-smoker chimneys make tremendous subjects for magazine covers, the proposal that submarine hydrothermal systems were involved in the origin of life has caused many investigators to focus on the eye-catching hydrothermal vents. In much the same way that tourists rush to watch the spectacular eruptions of Old Faithful geyser with little regard for the hydrology of the Yellowstone basin, attention is focused on the spectacular, high-temperature hydrothermal vents to the near exclusion of the enormous underlying hydrothermal systems. Nevertheless, the magnitude and complexity of geologic structures, heat flow, and hydrologic parameters which characterize the geyser basins at Yellowstone also characterize submarine hydrothermal systems. However, in the submarine systems the scale can be considerably more vast. Like Old Faithful, submarine hydrothermal vents have a spectacular quality, but they are only one fascinating aspect of enormous geologic systems operating at seafloor spreading centers throughout all of the ocean basins. A critical study of the possible role of hydrothermal processes in the origin of life should include the full spectrum of probable environments. The goals of this chapter are to synthesize diverse information about the inorganic geochemistry of submarine hydrothermal systems, assemble a description of the fundamental physical and chemical attributes of these systems, and consider the implications of high-temperature, fluid-driven processes for organic synthesis. Information about submarine hydrothermal systems comes from many directions. Measurements made directly on venting fluids provide useful, but remarkably limited, clues about processes operating at depth. The oceanic crust has been drilled to approximately 2.0 km depth providing many other pieces of information, but drilling technology has not allowed the bore holes and core samples to reach the maximum depths to which aqueous fluids circulate in oceanic crust. Such

  2. Evolving patterns of the fluids within the TAG hydrothermal field

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    The mixing of seawater/hydrothermal fluid within the large seafloor hydrothermal sulfide deposits plays a key role in the formation processes of the sulfide deposits.Some issues attract considerable attentions in the study of seafloor hydrothermal system in recent years,such as the relationships among different types of vent fluids,the characteristics of chemical compositions and mineral assemblages of the hydrothermal deposits and their governing factors.Combined with the measured data of hydrothermal fluid in the TAG field,the thermodynamic model of mixing processes of the heated seawater at different temperatures and the hydrothermal fluid is calculated to understand the precipitation mechanism of anhydrite and the genetic relationships between the black and white smoker fluids within the TAG mound.The results indicate that the heating of seawater and the mixing of hydrothermal fluid/seawater are largely responsible for anhydrite precipitation and the temperature of the heated seawater is not higher than 150 ℃ and the temperature of the end-member hydrothermal fluid is not lower than 400℃.Based on the simulated results,the evolving patterns of fluids within the TAG deposit are discussed.The mixed fluid of the end-member hydrothermal fluid and the seawater heated by wall rock undergoes conductive cooling during upflowing within the deposit and forms "White Smoker" eventually.In addition,the end-member hydrothermal fluid without mixed with seawater,but undergoing conductive cooling,vents out of the deposit and forms "Black Smoker".

  3. Voltammetric Investigation Of Hydrothermal Iron Speciation

    Directory of Open Access Journals (Sweden)

    Charlotte eKleint

    2016-05-01

    Full Text Available Hydrothermal vent fluids are highly enriched in iron (Fe compared to ambient seawater, and organic ligands may play a role in facilitating the transport of some hydrothermal Fe into the open ocean. This is important since Fe is a limiting micronutrient for primary production in large parts of the world`s surface ocean. We have investigated the concentration and speciation of Fe in several vent fluid and plume samples from the Nifonea vent field, Coriolis Troughs, New Hebrides Island Arc, South Pacific Ocean using competitive ligand exchange - adsorptive cathodic stripping voltammetry (CLE - AdCSV with salicylaldoxime (SA as the artificial ligand. Our results for total dissolved Fe (dFe in the buoyant hydrothermal plume samples showed concentrations up to 3.86 µM dFe with only a small fraction between 1.1% and 11.8% being chemically labile. Iron binding ligand concentrations ([L] were found in µM level with strong conditional stability constants up to log K[L],Fe3+ of 22.9. Within the non-buoyant hydrothermal plume above the Nifonea vent field, up to 84.7% of the available Fe is chemically labile and [L] concentrations up to 97 nM were measured. [L] was consistently in excess of Felab, indicating that all available Fe is being complexed, which in combination with high Felab values in the non-buoyant plume, signifies that a high fraction of hydrothermal dFe is potentially being transported away from the plume into the surrounding waters, contributing to the global oceanic Fe budget.

  4. Sulfur Metabolizing Microbes Dominate Microbial Communities in Andesite-Hosted Shallow-Sea Hydrothermal Systems

    OpenAIRE

    Zhang, Yao; Zhao, Zihao; Chen, Chen-Tung Arthur; Tang, Kai; Su, Jianqiang; Jiao, Nianzhi

    2012-01-01

    To determine microbial community composition, community spatial structure and possible key microbial processes in the shallow-sea hydrothermal vent systems off NE Taiwan’s coast, we examined the bacterial and archaeal communities of four samples collected from the water column extending over a redoxocline gradient of a yellow and four from a white hydrothermal vent. Ribosomal tag pyrosequencing based on DNA and RNA showed statistically significant differences between the bacterial and archaea...

  5. Comparative Composition, Diversity and Trophic Ecology of Sediment Macrofauna at Vents, Seeps and Organic Falls

    OpenAIRE

    Bernardino, Angelo F.; Levin, Lisa A; Thurber, Andrew R; Smith, Craig R.

    2012-01-01

    Sediments associated with hydrothermal venting, methane seepage and large organic falls such as whale, wood and plant detritus create deep-sea networks of soft-sediment habitats fueled, at least in part, by the oxidation of reduced chemicals. Biological studies at deep-sea vents, seeps and organic falls have looked at macrofaunal taxa, but there has yet to be a systematic comparison of the community-level attributes of sediment macrobenthos in various reducing ecosystems. Here we review key s...

  6. Measure Guideline: Passive Vents

    Energy Technology Data Exchange (ETDEWEB)

    Berger, David [Consortium for Advanced Residential Buildings, Norwalk, CT (United States); Neri, Robin [Consortium for Advanced Residential Buildings, Norwalk, CT (United States)

    2016-02-05

    This document addresses the use of passive vents as a source of outdoor air in multifamily buildings. The challenges associated with implementing passive vents and the factors affecting performance are outlined. A comprehensive design methodology and quantified performance metrics are provided. Two hypothetical design examples are provided to illustrate the process. This document is intended to be useful to designers, decision-makers, and contractors implementing passive ventilation strategies. It is also intended to be a resource for those responsible for setting high-performance building program requirements, especially pertaining to ventilation and outdoor air. To ensure good indoor air quality, a dedicated source of outdoor air is an integral part of high-performance buildings. Presently, there is a lack of guidance pertaining to the design and installation of passive vents, resulting in poor system performance. This report details the criteria necessary for designing, constructing, and testing passive vent systems to enable them to provide consistent and reliable levels of ventilation air from outdoors.

  7. Vent Relief Valve Test

    Science.gov (United States)

    2008-01-01

    Shown is the disassembly, examination, refurbishment and testing of the LH2 ( liquid hydrogen) and LOX (liquid oxygen) vent and relief valves for the S-IVB-211 engine stage in support of the Constellation/Ares project. This image is extracted from high definition video and is the highest resolution available.

  8. Theoretical constraints of physical and chemical properties of hydrothermal fluids on variations in chemolithotrophic microbial communities in seafloor hydrothermal systems

    Science.gov (United States)

    Nakamura, Kentaro; Takai, Ken

    2014-12-01

    In the past few decades, chemosynthetic ecosystems at deep-sea hydrothermal vents have received attention as plausible analogues to the early ecosystems of Earth, as well as to extraterrestrial ecosystems. These ecosystems are sustained by chemical energy obtained from inorganic redox substances (e.g., H2S, CO2, H2, CH4, and O2) in hydrothermal fluids and ambient seawater. The chemical and isotope compositions of the hydrothermal fluid are, in turn, controlled by subseafloor physical and chemical processes, including fluid-rock interactions, phase separation and partitioning of fluids, and precipitation of minerals. We hypothesized that specific physicochemical principles describe the linkages among the living ecosystems, hydrothermal fluids, and geological background in deep-sea hydrothermal systems. We estimated the metabolic energy potentially available for productivity by chemolithotrophic microorganisms at various hydrothermal vent fields. We used a geochemical model based on hydrothermal fluid chemistry data compiled from 89 globally distributed hydrothermal vent sites. The model estimates were compared to the observed variability in extant microbial communities in seafloor hydrothermal environments. Our calculations clearly show that representative chemolithotrophic metabolisms (e.g., thiotrophic, hydrogenotrophic, and methanotrophic) respond differently to geological and geochemical variations in the hydrothermal systems. Nearly all of the deep-sea hydrothermal systems provide abundant energy for organisms with aerobic thiotrophic metabolisms; observed variations in the H2S concentrations among the hydrothermal fluids had little effect on the energetics of thiotrophic metabolism. Thus, these organisms form the base of the chemosynthetic microbial community in global deep-sea hydrothermal environments. In contrast, variations in H2 concentrations in hydrothermal fluids significantly impact organisms with aerobic and anaerobic hydrogenotrophic metabolisms

  9. Depletion of a brine layer at the base of ridge-crest hydrothermal systems

    NARCIS (Netherlands)

    Schoofs, Stan; Hansen, Ulrich

    2001-01-01

    The variable salinity of fluid venting from mid-ocean ridges is indicative of mixing between hydrothermal seawater and fluids that have undergone supercritical phase separation. In order to study the stability of a brine-saturated layer that may form in the lowermost part of the hydrothermal system,

  10. Vente directe et terroir

    OpenAIRE

    Delfosse, Claire; Bernard, Cécile

    2009-01-01

    Si, dans la relation de proximité qu’établit la vente directe, il semble qu’il ne soit pas nécessaire de revendiquer le lien au terroir, il apparaît qu’aujourd’hui les nouvelles formes de vente directe et les formes « traditionnelles » renouvelées le font de plus en plus. Cela peut prendre différentes formes : revendication de la proximité, de la provenance « campagne », voire création de marques collectives faisant référence à un terroir ou un pays… Il s’agira donc dans cette communication d...

  11. Influence of CH4 and H2S availability on symbiont distribution, carbon assimilation and transfer in the dual symbiotic vent mussel Bathymodiolus azoricus

    NARCIS (Netherlands)

    Riou, V.; Halary, S.; Duperron, S.; Bouillon, S.; Elskens, M.; Bettencourt, R.; Santos, R.; Dehairs, F.; Colaço, A.

    2008-01-01

    High densities of mussels of the genus Bathymodiolus are present at hydrothermal vents of the Mid-Atlantic Ridge. It was previously proposed that the chemistry at vent sites would affect their sulphide- and methane-oxidizing endosymbionts' abundance. In this study, we confirmed the latter assumption

  12. Anhydrite precipitation in seafloor hydrothermal systems

    Science.gov (United States)

    Theissen-Krah, Sonja; Rüpke, Lars H.

    2016-04-01

    The composition and metal concentration of hydrothermal fluids venting at the seafloor is strongly temperature-dependent and fluids above 300°C are required to transport metals to the seafloor (Hannington et al. 2010). Ore-forming hydrothermal systems and high temperature vents in general are often associated with faults and fracture zones, i.e. zones of enhanced permeabilities that act as channels for the uprising hydrothermal fluid (Heinrich & Candela, 2014). Previous numerical models (Jupp and Schultz, 2000; Andersen et al. 2015) however have shown that high permeabilities tend to decrease fluid flow temperatures due to mixing with cold seawater and the resulting high fluid fluxes that lead to short residence times of the fluid near the heat source. A possible mechanism to reduce the permeability and thereby to focus high temperature fluid flow are mineral precipitation reactions that clog the pore space. Anhydrite for example precipitates from seawater if it is heated to temperatures above ~150°C or due to mixing of seawater with hydrothermal fluids that usually have high Calcium concentrations. We have implemented anhydrite reactions (precipitation and dissolution) in our finite element numerical models of hydrothermal circulation. The initial results show that the precipitation of anhydrite efficiently alters the permeability field, which affects the hydrothermal flow field as well as the resulting vent temperatures. C. Andersen et al. (2015), Fault geometry and permeability contrast control vent temperatures at the Logatchev 1 hydrothermal field, Mid-Atlantic Ridge, Geology, 43(1), 51-54. M. D. Hannington et al. (2010), Modern Sea-Floor Massive Sulfides and Base Metal Resources: Toward an Estimate of Global Sea-Floor Massive Sulfide Potential, in The Challenge of Finding New Mineral Resources: Global Metallogeny, Innovative Exploration, and New Discoveries, edited by R. J. Goldfarb, E. E. Marsh and T. Monecke, pp. 317-338, Society of Economic Geologists

  13. Preliminary Modeling of Two-Phase Flow at the Main Endeavour Vent Field

    Science.gov (United States)

    Singh, S.; Lowell, R. P.

    2011-12-01

    The high temperature hydrothermal vents of Main Endeavour Field (MEF), Juan de Fuca ridge exhibited quasi-steady North-South trending spatial gradients of both temperature and salinity for more than a decade before a magmatic event changed the vent characteristics. In order to explain these observations, we construct two-dimensional numerical models of two-phase hydrothermal flow of the MEF. We consider both along-axis and across-axis simulations, taking into account the vent field geometry and incorporating various parameters, such as different basal temperature distributions and permeability structures that might affect the vent fluid temperature and chemistry. Preliminary results from across-axis models, in which the basal temperature decreases linearly away from the ridge axis and results in a single high-temperature plume, indicate that basal temperature alone does not affect steady-state vent temperature and salinity of the vents. Simulations that include the presence of a high-permeability extrusive layer 2A atop the spreading ridge results in a zone of narrower and lower temperature venting. The effect of a low permeability zone of anhydrite would tend to mitigate the decrease in temperature, however. Along-axis simulations performed to date, with an extended uniform high temperature basal boundary, produce multiple plumes; but the plumes do not exhibit a strong along-axis gradient in vent salinity or temperature as observed at the MEF. These preliminary results suggest that the observed N-S gradient in temperature and salinity at MEF reflects interplay between heat source and either near the surface or deep-seated heterogeneous permeability structures. Three-dimensional simulations might ultimately be required to understand hydrothermal circulation at the MEF.

  14. Viral Genomics and Evolution in Subseafloor Diffuse Flow Viral Communities in the Main Endeavour Hydrothermal Field, Juan de Fuca Ridge

    Science.gov (United States)

    Anderson, R. E.; Baross, J. A.

    2010-04-01

    In the dynamic environment of hydrothermal vents, transduction may play a crucial role in microbial evolution. Metagenomic analysis of diffuse flow viral communities may elucidate the nature and extent of transduction in these ancient ecosystems.

  15. Clone and expression of the gene with a Ricin B-lectin domain of the tubeworm,Ridgeia piscesae,from the hydrothermal vent%深海热液区管状蠕虫 Ridgeiapiscesae中Ricin B-lectin型结构域基因的克隆与表达

    Institute of Scientific and Technical Information of China (English)

    徐海鹏; 阮灵伟; 崔治中

    2014-01-01

    Lectin,as an important family of pattern recognition receptors,has been applied widely in various fields. Therefore,development and utilization of new lectins are of great significance.In this study,a Ricin B-lectin gene rgal was first cloned from the tubeworm Ridgeia piscesae,which lives around the hydrothermal vent.Sequence anal-ysis revealed that RGAL had low homology with the known sequence.However,it contained two homologousβ-tre-foil fold typical of the Ricin B-lectin domains.These results suggested that RGAL might be a novel lectin.Further-more,the RGAL was cloned and recombinant expressed and the soluble proteins were obtained.The study will es-tablish a basis for further study on properties and functions of the RGAL.%作为一种重要的模式识别因子,凝集素在多个领域中均有着广泛的应用价值,因此,开发和利用新型凝集素将具有重要意义.本研究以生活在深海热液区极端环境条件下的管状蠕虫Ridgeia piscesae为材料,首次从管状蠕虫中克隆获得Ricin B-lectin基因 rgal.序列分析表明,RGAL与已知序列的相似性较低,其含有2个Ricin B-lectin型结构域,并且该结构域具有该家族所特有的β-三叶草形三维结构.多方面的分析结果显示RGAL可能是一个新颖的凝集素蛋白.对RGAL进行了原核重组表达,并对其复性条件进行了摸索优化,成功获得了其可溶性表达产物,为后续RGAL的生物活性分析奠定了坚实的基础.

  16. Filtered-vented containment systems

    International Nuclear Information System (INIS)

    The potential benefits of filtered-vented containment systems as a means for mitigating the effects of severe accidents are analyzed. Studies so far have focused upon two operating reactor plants in the United States, a large-containment pressurized water reactor and a Mark I containment boiling water reactor. Design options that could be retrofitted to these plants are described including single-component once-through venting systems, multiple-component systems with vent and recirculation capabilities, and totally contained venting systems. A variety of venting strategies are also described which include simple low-volume containment pressure relief strategies and more complicated, high-volume venting strategies that require anticipatory actions. The latter type of strategy is intended for accidents that produce containment-threatening pressure spikes

  17. Metagenome and Metatranscriptome Revealed a Highly Active Sulfur Cycle in an Oil-Immersed Hydrothermal Chimney in Guaymas Basin

    OpenAIRE

    Ying eHe; Xiaoyuan eFeng; Jing eFang; Yu eZhang; Xiang eXiao

    2015-01-01

    The hydrothermal vent system is a typical chemosynthetic ecosystem in which microorganisms play essential roles in the geobiochemical cycling. Although it has been well recognized that the inorganic sulfur compounds are abundant and actively converted through chemosynthetic pathways, the sulfur budget in a hydrothermal vent is poorly characterized due to the complexity of microbial sulfur cycling resulting from the numerous parties involved in the processes. In this study, we performed an int...

  18. Hydrothermal Plume Geochemistry along the East Lau Spreading Center

    Science.gov (United States)

    Resing, J. A.; Baker, E.; Martinez, F.; Buck, N.; Walker, S.; Seewald, J.; Proskurowski, G.; Lupton, J.; Wheat, G.

    2008-12-01

    In 2004 and 2008, we conducted extensive surveys of hydrothermal plumes along the East Lau Spreading Center (ELSC) in the Lau Back Arc Basin. The survey in 2008 is the most comprehensive plume survey conducted on any ridge to date. It covered 100 km2 by towing the 120 kHz side scan sonar and associated sensors from 19.9 to 21° S at 1 km intervals extending 5km on each side of the ridge crest. This part of the survey encompassed the known vent fields, Tow Cam, ABE, and Kila Moana. A fourth major vent field north of ABE was also identified. Near the ABE vent field survey lines were done at 0.5km intervals. The Valu Fa Ridge from 21.9 to 22.4°S was also surveyed using 7 lines at 0.7km spacing. The side scan sonar was towed ~ 100m above the sea floor with plume sensing instruments on the towline, clump weight, sonar, and on a line extended below the clump weight. Plume sensors included the Vents In Situ Analyzer, optical backscatter, Oxidation Reduction Potential, and CTD. Vertical and towed hydrocasts were located in areas of interest to collect sea water samples for chemical characterization of the plumes. While the surveys indicate that robust (high temperature) venting was restricted to an area within 1km of the ridge axis, there was some limited evidence of lower temperature venting further form the axis. The plume data from the sensors and discrete samples will be used to chemically characterize the hydrothermal plumes found along the ELSC. Emphasis will be placed on known vent fields and the North ABE field. 3He and Mn in the plumes will be compared to vent fluid data at the known sites. Although the N. ABE field appears to be the largest of the vent fields in this region, vent fluids from it have not yet been collected. The plumes above N. ABE have the most elevated levels of 3He and Mn of all of the plumes sampled along the ELSC and Valu Fa Ridges. This vent field is located closer to the transitions between andesitic rocks in the south and basaltic rocks in

  19. Effect of shallow-water venting in Azores on a few marine biota

    Digital Repository Service at National Institute of Oceanography (India)

    Colaco, A.; Raghukumar, C.; Mohandass, C.; Cardigos, F.; Santos, R.S.

    The 1000 m high D. Joao de Castro seamount lies in the middle of the Azores Archipelago (Portugal) on the hyperslow-spreading Terceira rift in the Atlantic. Hydrothermal vents were found near the top of the seamount and occurred in two distinct...

  20. Tornado protection by venting

    International Nuclear Information System (INIS)

    The purpose of this paper is to demonstrate the ability to protect a modern nuclear power plant from the effects of a tornado by the use of a system of venting in all safety-related structures outside of the containment. The paper demonstrates this by presenting a method of analysis and of equipment selection that fully complies with the intent and the letter of applicable federal regulatory guides. A report of an actual tornado in the City of Kalamazoo, Michigan, suggests that the concept of sealing a plant during a tornado may not always be applicable

  1. Copper speciation in deep exosystems : hydrothermal vents and cold seeps

    OpenAIRE

    Aumond, Virginie

    2013-01-01

    This work presents the copper speciation in the deep ocean and the exchanges existing between different size fractions : particulate, dissolved and colloidal. It focus on the mixing zone between deep sources and seawater. The enhancement of our knowledge and the optimization of instrumentation make possible the development of sampling and analysis strategies accorded with the nature and the accessibility of these extreme environments. A part of this work consisted in the development of effect...

  2. Heat flux estimates from the Gakkel Ridge 85E vent field from the AGAVE 2007 expedition

    Science.gov (United States)

    Stranne, C.; Winsor, P.; Sohn, R. A.; Liljebladh, B.

    2009-04-01

    During the Arctic Gakkel Vents Expedition (AGAVE) 2007, abundant hydrothermal venting was discovered on the Gakkel Ridge at 85E. Hydrothermal vents on the sea floor give rise to buoyant plumes which, when reaching neutral buoyancy, spreads horizontally over areas with length scales on the order of several kilometres and are therefore easily detected with a CTD rosette. The detected anomalies are consistent with the findings 6 years earlier during the Arctic Mid-Ocean Ridge Expedition (AMORE) 2001. The horizontal and vertical distribution of the anomalies is considered in order to establish the number of individual plumes detected. The objective of this paper is to estimate the minimum heat input required to reproduce the observed plumes, using a turbulent entrainment model. The model was run with a large number of combinations of boundary conditions (nozzle area, vertical velocity and temperature) in order to see which combinations that give rise to the observed plume characteristics (level of neutral buoyancy and temperature anomaly). For each individual plume, we estimate the minimum heat flux required to obtain the observed temperature anomaly. Adding the minimum heat flux from each vent together, the total heat flux for the vent field is estimated to be ~ 2 GW. The estimated value is comparable or larger than any other known vent field.

  3. Genome-resolved metagenomics reveals that sulfur metabolism dominates the microbial ecology of rising hydrothermal plumes

    Science.gov (United States)

    Anantharaman, K.; Breier, J. A., Jr.; Jain, S.; Reed, D. C.; Dick, G.

    2015-12-01

    Deep-sea hydrothermal plumes occur when hot fluids from hydrothermal vents replete with chemically reduced elements and compounds like sulfide, methane, hydrogen, ammonia, iron and manganese mix with cold, oxic seawater. Chemosynthetic microbes use these reduced chemicals to power primary production and are pervasive throughout the deep sea, even at sites far removed from hydrothermal vents. Although neutrally-buoyant hydrothermal plumes have been well-studied, rising hydrothermal plumes have received little attention even though they represent an important interface in the deep-sea where microbial metabolism and particle formation processes control the transformation of important elements and impact global biogeochemical cycles. In this study, we used genome-resolved metagenomic analyses and thermodynamic-bioenergetic modeling to study the microbial ecology of rising hydrothermal plumes at five different hydrothermal vents spanning a range of geochemical gradients at the Eastern Lau Spreading Center (ELSC) in the Western Pacific Ocean. Our analyses show that differences in the geochemistry of hydrothermal vents do not manifest in microbial diversity and community composition, both of which display only minor variance across ELSC hydrothermal plumes. Microbial metabolism is dominated by oxidation of reduced sulfur species and supports a diversity of bacteria, archaea and viruses that provide intriguing insights into metabolic plasticity and virus-mediated horizontal gene transfer in the microbial community. The manifestation of sulfur oxidation genes in hydrogen and methane oxidizing organisms hints at metabolic opportunism in deep-sea microbes that would enable them to respond to varying redox conditions in hydrothermal plumes. Finally, we infer that the abundance, diversity and metabolic versatility of microbes associated with sulfur oxidation impart functional redundancy that could allow it to persist in the dynamic settings of hydrothermal plumes.

  4. The magnetic signature of hydrothermal systems in slow spreading environments

    Science.gov (United States)

    Tivey, Maurice A.; Dyment, Jérôme

    Slow spreading mid-ocean ridges like the Mid-Atlantic Ridge host a remarkable diversity of hydrothermal systems including vent systems located on the neovolcanic axis, large axial volcanoes, in transform faults and nontransform offsets, and associated with low-angle detachment faults, now recognized as a major tectonic feature of slow spreading environments. Hydrothermal systems are hosted in various lithologies from basalt to serpentinized peridotite and exposed lower oceanic crust. The substantial variations of hydrothermal processes active in these environments have important implications for the magnetic structure of oceanic crust and upper mantle. Hydrothermal processes can both destroy the magnetic minerals in basalt, diabase, and gabbro and create magnetic minerals by serpentinization of ultramafic rocks and deposition of magnetic minerals. We report on the diversity of magnetic anomaly signatures over the vent systems at slow spreading ridges and show that the lateral scale of hydrothermal alteration is fundamentally a local phenomenon. This highly focused process leads to magnetic anomalies on the scale of individual vent fields, typically a few hundreds of meters or less in size. To detect such features, high-resolution, near-bottom magnetic surveys are required rather than sea surface surveys. High-resolution surveys are now more tractable with deep-towed systems, dynamically positioned ships, and with the recent development of autonomous underwater vehicles, which allow detailed mapping of the seafloor on a scale relevant to hydrothermal activity. By understanding these present-day active hydrothermal systems, we can explore for yet to be discovered buried deposits preserved off-axis, both to determine past history of hydrothermal activity and for resource assessment.

  5. Isotopic evidence of magmatism and a sedimentary carbon source at the Endeavour hydrothermal system

    Energy Technology Data Exchange (ETDEWEB)

    Brown, T A; Proskurowski, G; Lilley, M D

    2004-01-07

    Stable and radiocarbon isotope measurements made on CO{sub 2} from high temperature hydrothermal vents on the Endeavour Segment of the Juan de Fuca Ridge indicate both magmatic and sedimentary sources of carbon to the hydrothermal system. The Endeavour segment is devoid of overlying sediments and has shown no observable signs of surficial magmatic activity during the {approx}20 years of ongoing studies. The appearance of isotopically heavy, radiocarbon dead CO{sub 2} after a 1999 earthquake swarm requires that this earthquake event was magmatic in origin. Evidence for a sedimentary organic carbon source suggests the presence of buried sediments at the ridge axis. These findings, which represent the first temporally coherent set of radiocarbon measurements from hydrothermal vent fluids, demonstrate the utility of radiocarbon analysis in hydrothermal studies. The existence of a sediment source at Endeavour and the occurrence of magmatic episodes illustrate the extremely complex and evolving nature of the Endeavour hydrothermal system.

  6. Heat and mass flux estimation of modern seafloor hydrothermal activity

    Institute of Scientific and Technical Information of China (English)

    ZHAI Shikui; WANG Xingtao; YU Zenghui

    2006-01-01

    Research on heat and mass flux yielded by modern seafloor hydrothermal activity is very important, because it is involved not only in the base of ocean environment research, but also in the historical evolution of seawater properties. Currently, estimating heat flux is based on the observation data of hydrothermal smokers, low-temperature diffusive flow and mid-ocean ridge mainly. But there are some faults, for example, there is lack of a concurrent conductive item in estimating the heat flux by smokers and the error between the half-space cooling model and the observation data is too large. So, three kinds of methods are applied to re-estimating the heat flux of hydrothermal activity resepectively, corresponding estimation is 97.359 GW by hydrothermal smoker and diffusive flow, 84.895 GW by hydrothermal plume, and 4.11 TW by exponential attenuation method put forward by this paper. Research on mass flux estimation is relatively rare, the main reason for this is insufficient field observation data. Mass fluxes of different elements are calculated using hydrothermal vent fluid data from the TAG hydrothermal area on the Mid-Atlantic Ridge for the first time. Difference of estimations by different methods reflects the researching extent of hydrothermal activity, and systematically in-situ observation will help to estimate the contribution of hydrothermal activity to ocean chemical environment, ocean circulation and global climate precisely.

  7. Hydrothermal Liquefaction of Biomass

    Energy Technology Data Exchange (ETDEWEB)

    Elliott, Douglas C.

    2010-12-10

    collaboration with Canada to investigate kelp (seaweed) as a biomass feedstock. The collaborative project includes process testing of the kelp in HydroThermal Liquefaction in the bench-scale unit at PNNL. HydroThermal Liquefaction at PNNL is performed in the hydrothermal processing bench-scale reactor system. Slurries of biomass are prepared in the laboratory from whole ground biomass materials. Both wet processing and dry processing mills can be used, but the wet milling to final slurry is accomplished in a stirred ball mill filled with angle-cut stainless steel shot. The PNNL HTL system, as shown in the figure, is a continuous-flow system including a 1-litre stirred tank preheater/reactor, which can be connected to a 1-litre tubular reactor. The product is filtered at high-pressure to remove mineral precipitate before it is collected in the two high-pressure collectors, which allow the liquid products to be collected batchwise and recovered alternately from the process flow. The filter can be intermittently back-flushed as needed during the run to maintain operation. By-product gas is vented out the wet test meter for volume measurement and samples are collected for gas chromatography compositional analysis. The bio-oil product is analyzed for elemental content in order to calculate mass and elemental balances around the experiments. Detailed chemical analysis is performed by gas chromatography-mass spectrometry and 13-C nuclear magnetic resonance is used to evaluate functional group types in the bio-oil. Sufficient product is produced to allow subsequent catalytic hydroprocessing to produce liquid hydrocarbon fuels. The product bio-oil from hydrothermal liquefaction is typically a more viscous product compared to fast pyrolysis bio-oil. There are several reasons for this difference. The HTL bio-oil contains a lower level of oxygen because of more extensive secondary reaction of the pyrolysis products. There are less amounts of the many light oxygenates derived from the

  8. Characterization of Magma-Driven Hydrothermal Systems at Oceanic Spreading Centers

    Science.gov (United States)

    Farough, A.; Lowell, R. P.; Corrigan, R.

    2012-12-01

    Fluid circulation in high-temperature hydrothermal systems involves complex water-rock chemical reactions and phase separation. Numerical modeling of reactive transport in multi-component, multiphase systems is required to obtain a full understanding of the characteristics and evolution of hydrothermal vent systems. We use a single-pass parameterized model of high-temperature hydrothermal circulation at oceanic spreading centers constrained by observational parameters such as vent temperature, heat output, and vent field area, together with surface area and depth of the sub-axial magma chamber, to deduce fundamental hydrothermal parameters such as mass flow rate, bulk permeability, conductive boundary layer thickness at the base of the system, magma replenishment rate, and residence time in the discharge zone. All of these key subsurface characteristics are known for fewer than 10 sites out of 300 known hydrothermal systems. The principal limitations of this approach stem from the uncertainty in heat output and vent field area. For systems where data are available on partitioning of heat and chemical output between focused and diffuse flow, we determined the fraction of high-temperature vent fluid incorporated into diffuse flow using a two-limb single pass model. For EPR 9°50` N and ASHES, the diffuse flow temperatures calculated assuming conservative mixing are nearly equal to the observed temperatures indicating that approximately 80%-90% of the hydrothermal heat output occurs as high-temperature flow derived from magmatic heat even though most of the heat output appears as low-temperature diffuse discharge. For the Main Endeavour Field and Lucky Strike, diffuse flow fluids show significant conductive cooling and heating respectively. Finally, we calculate the transport of various geochemical constituents in focused and diffuse flow at the vent field scale and compare the results with estimates of geochemical transports from the Rainbow hydrothermal field where

  9. Preservation of iron(II) by carbon-rich matrices in a hydrothermal plume

    Energy Technology Data Exchange (ETDEWEB)

    Toner, Brandy M.; Fakra, Sirine C.; Manganini, Steven J.; Santelli, Cara M.; Marcus, Matthew A.; Moffett, James W.; Rouxel, Olivier; German, Christopher R.; Edwards, Katrina J.

    2008-09-20

    Hydrothermal venting associated with mid-ocean ridge volcanism is globally widespread. This venting is responsible for a dissolved iron flux to the ocean that is approximately equal to that associated with continental riverine runoff. For hydrothermal fluxes, it has long been assumed that most of the iron entering the oceans is precipitated in inorganic forms. However, the possibility of globally significant fluxes of iron escaping these mass precipitation events and entering open-ocean cycles is now being debated, and two recent studies suggest that dissolved organic ligands might influence the fate of hydrothermally vented metals. Here we present spectromicroscopic measurements of iron and carbon in hydrothermal plume particles at the East Pacific Rise mid-ocean ridge. We show that organic carbon-rich matrices, containing evenly dispersed iron(II)-rich materials, are pervasive in hydrothermal plume particles. The absence of discrete iron(II) particles suggests that the carbon and iron associate through sorption or complexation. We suggest that these carbon matrices stabilize iron(II) released from hydrothermal vents in the region, preventing its oxidation and/or precipitation as insoluble minerals. Our findings have implications for deep-sea biogeochemical cycling of iron, a widely recognized limiting nutrient in the oceans.

  10. Efficient and durable oxygen reduction and evolution of a hydrothermally synthesized La(Co0.55Mn0.45)0.99O3-δ nanorod/graphene hybrid in alkaline media

    Science.gov (United States)

    Ge, Xiaoming; Goh, F. W. Thomas; Li, Bing; Hor, T. S. Andy; Zhang, Jie; Xiao, Peng; Wang, Xin; Zong, Yun; Liu, Zhaolin

    2015-05-01

    The increasing global energy demand and the depletion of fossil fuels have stimulated intense research on fuel cells and batteries. Oxygen electrocatalysis plays essential roles as the electrocatalytic reduction and evolution of di-oxygen are always the performance-limiting factors of these devices relying on oxygen electrochemistry. A novel perovskite with the formula La(Co0.55Mn0.45)0.99O3-δ (LCMO) is designed from molecular orbital principles. The hydrothermally synthesized LCMO nanorods have unique structural and chemical properties and possess high intrinsic activities for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). The synergic covalent coupling between LCMO and NrGO enhances the bifunctional ORR and OER activities of the novel LCMO/NrGO hybrid catalyst. The ORR activity of LCMO/NrGO is comparable to the state-of-the-art Pt/C catalyst and its OER activity is competitive to the state-of-the-art Ir/C catalyst. LCMO/NrGO generally outperforms Pt/C and Ir/C with better bifunctional ORR and OER performance and operating durability. LCMO/NrGO represents a new class of low-cost, efficient and durable electrocatalysts for fuel cells, water electrolysers and batteries.The increasing global energy demand and the depletion of fossil fuels have stimulated intense research on fuel cells and batteries. Oxygen electrocatalysis plays essential roles as the electrocatalytic reduction and evolution of di-oxygen are always the performance-limiting factors of these devices relying on oxygen electrochemistry. A novel perovskite with the formula La(Co0.55Mn0.45)0.99O3-δ (LCMO) is designed from molecular orbital principles. The hydrothermally synthesized LCMO nanorods have unique structural and chemical properties and possess high intrinsic activities for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). The synergic covalent coupling between LCMO and NrGO enhances the bifunctional ORR and OER activities of the novel LCMO/NrGO hybrid

  11. Methane and radioactive isotopes in submarine hydrothermal systems

    International Nuclear Information System (INIS)

    This thesis consists of two parts: 1) methane and 2) radioactive isotopes, especially radon, in submarine hydrothermal systems. Both parts deal with the use of these gases as tracers for mapping hydrothermal vents at sea, and with their relationships to other sensitive tracers such as helium, manganese, and temperature. Hydrothermal methane was used as a real-time tracer for locating new submarine hydrothermal systems along spreading axes, discovering new hydrothermal systems at two locations in Pacific Ocean: 1) 200S on East Pacific Rise, and 2) Mariana Trough Back-arc Basin. Methane shows good correlations with helium-3 and temperature with similar ratios in various hydrothermal systems, 3 to 42 x 106 for the methane to helium-3 ratio, and 3 to 19 μ cc/kg0C for the methane to temperature anomaly. These similar ratios from different areas provide evidence for chemical homogeneity of submarine hydrothermal waters. A good correlation between methane and manganese appears to be associated only with high-temperature hydrothermal systems. Radioisotopes in the vent waters of 210N high-temperature hydrothermal system have end-member concentrations of 7.5 to 40 dpm/kg for Ra-226, 360 dpm/kg for Rn 222, 62 dpm/kg for Pb-210, and 19 dpm/kg for Po-210. The radon activity for this system is one order of magnitude lower, and the Pb-210 activity is one order or magnitude higher, than those a the low temperature Galapagos system. All these observations suggest that the high radon, and low Pb-210 activity observed in Galapagos system may originate from the extensive subsurface mixing and water-rock interaction in this system (direct injection of radon and scavenging of Pb-210)

  12. A high-temperature hydrothermal deposit on the East Pacific Rise near 70N

    International Nuclear Information System (INIS)

    A SEABEAM survey of the East Pacific Rise (EPR) led to the selection of several sites having structural characteristics favorable for hydrothermal activity. Dredging of such an area located at 70 N on the EPR resulted in the recovery of sulfides, oxides and fresh basalt. Chemical analyses and isotopic compositions showed that the recovered pyrites were probably precipitated directly from hot vent hydrothermal waters. Chemical analyses and isotopic composition of manganese-iron oxides indicated that they too were of hydrothermal origin. 210Pb/Pb measurements yielded ages of 90 +- 10 years for the deposits. This site may still be undergoing hydrothermal activity. (orig.)

  13. The metatranscriptome of a deep-sea hydrothermal plume is dominated by water column methanotrophs and lithotrophs

    OpenAIRE

    Lesniewski, Ryan A; Jain, Sunit; Anantharaman, Karthik; Schloss, Patrick D.; Dick, Gregory J.

    2012-01-01

    Microorganisms mediate geochemical processes in deep-sea hydrothermal vent plumes, which are a conduit for transfer of elements and energy from the subsurface to the oceans. Despite this important microbial influence on marine geochemistry, the ecology and activity of microbial communities in hydrothermal plumes is largely unexplored. Here, we use a coordinated metagenomic and metatranscriptomic approach to compare microbial communities in Guaymas Basin hydrothermal plumes to background water...

  14. Diversity and phylogenetic analyses of bacteria from a shallow-waterhydrothermal vent in Milos island (Greece

    Directory of Open Access Journals (Sweden)

    Donato eGiovannelli

    2013-07-01

    Full Text Available Studies of shallow-water hydrothermal vents have been lagging behind their deep-sea counterparts. Hence, the importance of these systems and their contribution to the local and regional diversity and biogeochemistry is unclear. This study analyzes the bacterial community along a transect at the shallow-water hydrothermal vent system of Milos island, Greece. The abundance and biomass of the prokaryotic community is comparable to areas not affected by hydrothermal activity and was, on average, 1.34×108 cells g-1. The abundance, biomass and diversity of the prokaryotic community increased with the distance from the center of the vent and appeared to be controlled by the temperature gradient rather than the trophic conditions. The retrieved 16S rRNA gene fragments matched sequences from a variety of geothermal environments, although the average similarity was low (94 %, revealing previously undiscovered taxa. Epsilonproteobacteria constituted the majority of the population along the transect, with an average contribution to the total diversity of 60%. The larger cluster of 16S rRNA gene sequences was related to chemolithoautotrophic Sulfurovum spp., an Epsilonproteobacterium so far detected only at deep-sea hydrothermal vents. The presence of previously unknown lineages of Epsilonproteobacteria could be related to the abundance of organic matter in these systems, which may support alternative metabolic strategies to chemolithoautotrophy. The relative contribution of Gammaproteobacteria to the Milos microbial community increased along the transect as the distance from the center of the vent increased. Further attempts to isolate key species from these ecosystems will be critical to shed light on their evolution and ecology.

  15. Optical signatures of dissolved organic matter from the Endeavour and Axial vent fields

    Science.gov (United States)

    Stubbins, A.; Butterfield, D.; Rossel, P. E.; Dittmar, T.

    2011-12-01

    Recent studies have revealed that hydrothermal systems in the deep ocean are both sources and processors of dissolved organic matter (DOM). Sub-floor stores of fossil organic carbon may be exported to the deep ocean directly adding fossil C to the deep ocean dissolved organic carbon (DOC) pool and altering its apparent age. Fossil methane and carbon dioxide are also exported from vents. These C sources can then be utilized by chemotrophs and later enter the DOM pool as fossil DOC. Finally, when deep ocean waters are entrained into vent systems, the resultant heating may alter the chemical and optical properties of the DOM in these deep ocean waters. Dissolved organic matter (DOM) samples were collected from vents ranging in temperature from 10 to over 300 degrees centigrade across the Endeavour and Axial fields along the Juan de Fuca ridge. Elevated DOC and protein-like fluorescence reveal the vents to fuel the chemotrophic production of organic matter either in the adjacent water column or local sediments. High DOC and increased humic-like fluorescence in the hottest vent fluids, suggests the thermal degradation of DOM either from buried fossil sources or the entrainment of local waters enriched in DOC due to chemotrophic productivity. Natural and radio-carbon analyses are underway and will provide further insight into the ultimate source of this colored, fluorescent hydrothermal DOM.

  16. Cement penetration after patella venting.

    Science.gov (United States)

    Jones, Christopher W; Lam, Li-On; Butler, Adam; Wood, David J; Walsh, William R

    2009-01-01

    There is a high rate of patellofemoral complications following total knee arthroplasty. Optimization of the cement-bone interface by venting and suction of the tibial plateau has been shown to improve cement penetration. Our study was designed to investigate if venting the patella prior to cementing improved cement penetration. Ten paired cadaver patellae were allocated prior to resurfacing to be vented or non-vented. Bone mineral density (BMD) was measured by DEXA scanning. In vented specimens, a 1.6 mm Kirschner wire was used to breach the anterior cortex at the center. Specimens were resurfaced with standard Profix instrumentation and Versabond bone cement (Smith and Nephew PLC, UK). Cement penetration was assessed from Faxitron and sectioned images by a digital image software package (ImageJ V1.38, NIH, USA). Wilcoxon rank sum test was used to assess the difference in cement penetration between groups. The relationship between BMD and cement penetration was analyzed by Pearson correlation coefficient. There was a strong negative correlation between peak BMD and cement penetration when analyzed independent of experimental grouping (r(2)=-0.812, p=0.004). Wilcoxon rank sum testing demonstrated no significant difference (rank sum statistic W=27, p=0.579) in cement penetration between vented (10.53%+/-4.66; mean+/-std dev) and non-vented patellae (11.51%+/-6.23; mean+/-std dev). Venting the patella using a Kirschner wire does not have a significant effect on the amount of cement penetration achieved in vitro using Profix instrumentation and Versabond cement. PMID:19010682

  17. Vente d'artisanat

    CERN Multimedia

    Staff Associaiton

    2014-01-01

      Éducation et Libération Vente d’artisanat du Tiers Monde Mardi 22 et mercredi 23 avril 2014 CERN, Bâtiment principal Togo, École Arc en ciel, construction des salles de classe. Appel pour le financement de ce chantier afin de libérer l’école de la charge des loyers payés pendant des années. Après nos réalisations en Amérique latine et au Bénin, nous mobilisons nos efforts pour l’école Arc en ciel de Kpémé, au Togo, sur les bords de l’Océan, à mi-chemin entre Lomé et la frontière entre le Bénin et le Togo. Il s’agit d’une école primaire privée, laïque qui a très bonne réputation en termes de résultats, notamment pour les écoliers en fin de scolar...

  18. Metagenome and Metatranscriptome Revealed a Highly Active and Intensive Sulfur Cycle in an Oil-Immersed Hydrothermal Chimney in Guaymas Basin

    OpenAIRE

    He, Ying; Feng, Xiaoyuan; Fang, Jing; Zhang, Yu; Xiao, Xiang

    2015-01-01

    The hydrothermal vent system is a typical chemosynthetic ecosystem in which microorganisms play essential roles in the geobiochemical cycling. Although it has been well-recognized that the inorganic sulfur compounds are abundant and actively converted through chemosynthetic pathways, the sulfur budget in a hydrothermal vent is poorly characterized due to the complexity of microbial sulfur cycling resulting from the numerous parties involved in the processes. In this study, we performed an int...

  19. Monitoring Endeavour vent field deep-sea ecosystem dynamics through NEPTUNE Canada seafloor observatory

    Science.gov (United States)

    Matabos, M.; NC Endeavour Science Team

    2010-12-01

    Mid-ocean ridges are dynamic systems where the complex linkages between geological, biological, chemical, and physical processes are not yet well understood. Indeed, the poor accessibility to the marine environment has greatly limited our understanding of deep-sea ecosystems. Undersea cabled observatories offer the power and bandwidth required to conduct long-term and high-resolution time-series observations of the seafloor. Investigations of mid-ocean ridge hydrothermal ecosystem require interdisciplinary studies to better understand the dynamics of vent communities and the physico-chemical forces that influence them. NEPTUNE Canada (NC) regional observatory is located in the Northeast Pacific, off Vancouver Island (BC, Canada), and spans ecological environments from the beach to the abyss. In September-October 2010, NC will be instrumenting its 5th node, including deployment of a multi-disciplinary suite of instruments in two vent fields on the Endeavour Segment of the Juan de Fuca Ridge. These include a digital camera, an imaging sonar for vent plumes and flow characteristics (i.e. COVIS), temperature resistivity probes, a water sampler and seismometers. In 2011, the TEMPO-mini, a new custom-designed camera and sensor package created by IFREMER for real-time monitoring of hydrothermal faunal assemblages and their ecosystems (Sarrazin et al. 2007), and a microbial incubator, will added to the network in the Main Endeavour and Mothra vent fields. This multidisciplinary approach will involve a scientific community from different institutions and countries. Significant experience aids in this installation. For example, video systems connected to VENUS and NC have led to the development of new experimental protocols for time-series observations using seafloor cameras, including sampling design, camera calibration and image analysis methodologies (see communication by Aron et al. and Robert et al.). Similarly, autonomous deployment of many of the planned instruments

  20. Toward an appreciation of hydrothennal-vent animals: Their environment, physiological ecology, and tissue stable isotope values

    Science.gov (United States)

    Fisher, Charles R.

    In the last few years several comprehensive reviews of the biology of hydrothermal vent organisms and communities have been published. In this contribution I will not attempt to exhaustively review the literature, list the fauna, or the known sites, but rather present a conceptual basis for understanding the relation between the dominant metazoan "primary producers" in hydrothermal vent communities and their environment. In addition to the other chapters in this volume, interested readers are encouraged to consult the following reviews for a more detailed discussion of particular aspects of vent biology. The community ecology of hydrothermal vents is reviewed by Grassle [1986], Tunnicliffe [1991], and Lutz and Kennish [1993]. Tunnicliffe [1991] contains the most complete species lists and general site descriptions currently available. Fisher [1990] reviews the literature on chemoautotrophic symbioses and presents species lists of the hosts to chemoautotrophic symbionts known at that time. Those lists are updated in Nelson and Fisher [1995] and the physiology of the associations reviewed from a distinctly bacterial (symbiont) viewpoint. The 1992 review by Childress and Fisher takes a detailed look at the physiology of vent fauna, with a full coverage of subjects such as rate processes, blood function, and chemical composition, which are not covered in depth in the other reviews, but are of special relevance to this contribution. Uses (and abuses) of stable isotopes are discussed in several of the above reviews, and are also reviewed specifically by Conway et al. [1994], Fiala-Médioni et al. [1993], and Kennicutt et al. [1992].

  1. Evolutionary strategies of cells and viruses in deep-sea hydrothermal systems revealed through comparative metagenomics

    Science.gov (United States)

    Anderson, R.; Sogin, M. L.; Baross, J. A.

    2013-12-01

    The deep-sea hydrothermal vent habitat hosts a diverse community of archaea and bacteria that withstand extreme fluctuations in environmental conditions. Abundant viruses in these systems must also withstand these environmental extremes, and a high proportion of viruses in these systems are lysogenic. Comparative analysis of a cellular and viral metagenome from a diffuse flow hydrothermal vent has provided insights into the evolutionary strategies of both cells and viruses in hydrothermal systems. We detected numerous mobile elements in the viral and cellular gene pools as well as a large number of prophage in the cellular fraction. We show that the hydrothermal vent viral gene pool is relatively enriched in genes related to energy metabolism, a feature that is unique to the hydrothermal vent viral gene pool compared to viral gene pools from other environments, indicating a potential for integrated prophage to enhance host metabolic flexibility. We also detected stronger purifying selection in the viral versus cellular gene pool, indicating selection pressures that promote prolonged viral integration in the host. Our results support the hypothesis that viruses enhance host genomic plasticity and adaptability in this extreme and dynamic environment. Finally, we will discuss general implications of this work for understanding the viral impact on biogeochemical cycles and evolutionary trajectories of microbial populations in the deep subsurface biosphere.

  2. Composition of hydrothermal fluids and mineralogy of associated chimney material on the East Scotia Ridge back-arc spreading centre

    Science.gov (United States)

    James, Rachael H.; Green, Darryl R. H.; Stock, Michael J.; Alker, Belinda J.; Banerjee, Neil R.; Cole, Catherine; German, Christopher R.; Huvenne, Veerle A. I.; Powell, Alexandra M.; Connelly, Douglas P.

    2014-08-01

    The East Scotia Ridge is an active back-arc spreading centre located to the west of the South Sandwich island arc in the Southern Ocean. Initial exploration of the ridge by deep-tow surveys provided the first evidence for hydrothermal activity in a back-arc setting outside of the western Pacific, and we returned in 2010 with a remotely operated vehicle to precisely locate and sample hydrothermal sites along ridge segments E2 and E9. Here we report the chemical and isotopic composition of high- and low-temperature vent fluids, and the mineralogy of associated high-temperature chimney material, for two sites at E2 (Dog’s Head and Sepia), and four sites at E9 (Black & White, Ivory Tower, Pagoda and Launch Pad). The chemistry of the fluids is highly variable between the ridge segments. Fluid temperatures were ∼350 °C at all vent sites except Black & White, which was significantly hotter (383 °C). End-member chloride concentrations in E2 fluids (532-536 mM) were close to background seawater (540 mM), whereas Cl in E9 fluids was much lower (98-220 mM) indicating that these fluids are affected by phase separation. Concentrations of the alkali elements (Na, Li, K and Cs) and the alkaline earth elements (Ca, Sr and Ba) co-vary with Cl, due to charge balance constraints. Similarly, concentrations of Mn and Zn are highest in the high Cl fluids but, by contrast, Fe/Cl ratios are higher in E9 fluids (3.8-8.1 × 10-3) than they are in E2 fluids (1.5-2.4 × 10-3) and fluids with lowest Cl have highest Cu. Although both ridge segments are magmatically inflated, there is no compelling evidence for input of magmatic gases to the vent fluids. Fluid δD values range from 0.2‰ to 1.5‰, pH values (3.02-3.42) are not especially low, and F concentrations (34.6-54.4 μM) are lower than bottom seawater (62.8 μM). The uppermost sections of conjugate chimney material from E2, and from Ivory Tower and Pagoda at E9, typically exhibit inner zones of massive chalcopyrite enclosed

  3. Sources of organic nitrogen at the serpentinite-hosted Lost City hydrothermal field.

    Science.gov (United States)

    Lang, S Q; Früh-Green, G L; Bernasconi, S M; Butterfield, D A

    2013-03-01

    The reaction of ultramafic rocks with water during serpentinization at moderate temperatures results in alkaline fluids with high concentrations of reduced chemical compounds such as hydrogen and methane. Such environments provide unique habitats for microbial communities capable of utilizing these reduced compounds in present-day and, possibly, early Earth environments. However, these systems present challenges to microbial communities as well, particularly due to high fluid pH and possibly the availability of essential nutrients such as nitrogen. Here we investigate the source and cycling of organic nitrogen at an oceanic serpentinizing environment, the Lost City hydrothermal field (30°N, Mid-Atlantic Ridge). Total hydrolizable amino acid (THAA) concentrations in the fluids range from 736 to 2300 nm and constitute a large fraction of the dissolved organic carbon (2.5-15.1%). The amino acid distributions, and the relative concentrations of these compounds across the hydrothermal field, indicate they most likely derived from chemolithoautotrophic production. Previous studies have identified the presence of numerous nitrogen fixation genes in the fluids and the chimneys. Organic nitrogen in actively venting chimneys has δ(15) N values as low as 0.1‰ which is compatible with biological nitrogen fixation. Total hydrolizable amino acids in the chimneys are enriched in (13) C by 2-7‰ compared to bulk organic matter. The distribution and absolute δ(13) C(THAA) values are compatible with a chemolithoautotrophic source, an attribution also supported by molar organic C/N ratios in most active chimneys (4.1-5.5) which are similar to those expected for microbial communities. In total, these data indicate nitrogen is readily available to microbial communities at Lost City. PMID:23346942

  4. Sulfur metabolizing microbes dominate microbial communities in Andesite-hosted shallow-sea hydrothermal systems.

    Science.gov (United States)

    Zhang, Yao; Zhao, Zihao; Chen, Chen-Tung Arthur; Tang, Kai; Su, Jianqiang; Jiao, Nianzhi

    2012-01-01

    To determine microbial community composition, community spatial structure and possible key microbial processes in the shallow-sea hydrothermal vent systems off NE Taiwan's coast, we examined the bacterial and archaeal communities of four samples collected from the water column extending over a redoxocline gradient of a yellow and four from a white hydrothermal vent. Ribosomal tag pyrosequencing based on DNA and RNA showed statistically significant differences between the bacterial and archaeal communities of the different hydrothermal plumes. The bacterial and archaeal communities from the white hydrothermal plume were dominated by sulfur-reducing Nautilia and Thermococcus, whereas the yellow hydrothermal plume and the surface water were dominated by sulfide-oxidizing Thiomicrospira and Euryarchaeota Marine Group II, respectively. Canonical correspondence analyses indicate that methane (CH(4)) concentration was the only statistically significant variable that explains all community cluster patterns. However, the results of pyrosequencing showed an essential absence of methanogens and methanotrophs at the two vent fields, suggesting that CH(4) was less tied to microbial processes in this shallow-sea hydrothermal system. We speculated that mixing between hydrothermal fluids and the sea or meteoric water leads to distinctly different CH(4) concentrations and redox niches between the yellow and white vents, consequently influencing the distribution patterns of the free-living Bacteria and Archaea. We concluded that sulfur-reducing and sulfide-oxidizing chemolithoautotrophs accounted for most of the primary biomass synthesis and that microbial sulfur metabolism fueled microbial energy flow and element cycling in the shallow hydrothermal systems off the coast of NE Taiwan. PMID:22970260

  5. Sulfur metabolizing microbes dominate microbial communities in Andesite-hosted shallow-sea hydrothermal systems.

    Directory of Open Access Journals (Sweden)

    Yao Zhang

    Full Text Available To determine microbial community composition, community spatial structure and possible key microbial processes in the shallow-sea hydrothermal vent systems off NE Taiwan's coast, we examined the bacterial and archaeal communities of four samples collected from the water column extending over a redoxocline gradient of a yellow and four from a white hydrothermal vent. Ribosomal tag pyrosequencing based on DNA and RNA showed statistically significant differences between the bacterial and archaeal communities of the different hydrothermal plumes. The bacterial and archaeal communities from the white hydrothermal plume were dominated by sulfur-reducing Nautilia and Thermococcus, whereas the yellow hydrothermal plume and the surface water were dominated by sulfide-oxidizing Thiomicrospira and Euryarchaeota Marine Group II, respectively. Canonical correspondence analyses indicate that methane (CH(4 concentration was the only statistically significant variable that explains all community cluster patterns. However, the results of pyrosequencing showed an essential absence of methanogens and methanotrophs at the two vent fields, suggesting that CH(4 was less tied to microbial processes in this shallow-sea hydrothermal system. We speculated that mixing between hydrothermal fluids and the sea or meteoric water leads to distinctly different CH(4 concentrations and redox niches between the yellow and white vents, consequently influencing the distribution patterns of the free-living Bacteria and Archaea. We concluded that sulfur-reducing and sulfide-oxidizing chemolithoautotrophs accounted for most of the primary biomass synthesis and that microbial sulfur metabolism fueled microbial energy flow and element cycling in the shallow hydrothermal systems off the coast of NE Taiwan.

  6. Marine shallow hydrothermal systems: imprint of their exclusive biogeochemistry on dissolved organic matter and chemosynthesis

    OpenAIRE

    Gómez Sáez, Gonzalo Vicente

    2016-01-01

    Shallow submarine hydrothermal systems are extreme environments with unique biogeochemical conditions, originating from (1) the interaction of hot, reduced fluids and cold, oxygenated seawater, and (2) the possibility of simultaneous primary production by photo- and chemosynthesis. The flux of carbon, reduced molecules and trace elements from hydrothermal vents is mainly controlled by dissolved organic matter (DOM), which is one of the largest pools of organic carbon in the oceans and therefo...

  7. ALP (Alkaline Phosphatase) Test

    Science.gov (United States)

    ... Also known as: ALK PHOS; Alkp Formal name: Alkaline Phosphatase Related tests: AST ; ALT ; GGT ; Bilirubin ; Liver Panel ; Bone Markers ; Alkaline Phosphatase Isoenzymes; Bone Specific ALP All content on ...

  8. Near shore submarine hydrothermal activity in Bahia Banderas, western Mexico

    OpenAIRE

    F. J. Núñez Cornú; Rosa María Prol Ledesma; A. Cupul Magaña; C. Suárez Plascencia

    2000-01-01

    Shallow submarine hydrothermal activity was detected in the Bahía de Banderas area, Mexico. Volcanic-type tremors were recorded by portable seismological stations onshore. Vent samples suggest a depositional sequence dominated by carbonates in the first stage (calcite and dolomite), followed by apatite and late barite veins. Layers of sequential deposition of sulfides were also observed, and are interpreted as cyclic variations of sulphur fugacity.

  9. Discovery of new hydrothermal activity and chemosynthetic fauna on the Central Indian Ridge at 18°-20° S.

    Directory of Open Access Journals (Sweden)

    Kentaro Nakamura

    Full Text Available Indian Ocean hydrothermal vents are believed to represent a novel biogeographic province, and are host to many novel genera and families of animals, potentially indigenous to Indian Ocean hydrothermal systems. In particular, since its discovery in 2001, much attention has been paid to a so-called 'scaly-foot' gastropod because of its unique iron-sulfide-coated dermal sclerites and the chemosynthetic symbioses in its various tissues. Despite increasing interest in the faunal assemblages at Indian Ocean hydrothermal vents, only two hydrothermal vent fields have been investigated in the Indian Ocean. Here we report two newly discovered hydrothermal vent fields, the Dodo and Solitaire fields, which are located in the Central Indian Ridge (CIR segments 16 and 15, respectively. Chemosynthetic faunal communities at the Dodo field are emaciated in size and composition. In contrast, at the Solitaire field, we observed faunal communities that potentially contained almost all genera found at CIR hydrothermal environments to date, and even identified previously unreported taxa. Moreover, a new morphotype of 'scaly-foot' gastropod has been found at the Solitaire field. The newly discovered 'scaly-foot' gastropod has similar morphological and anatomical features to the previously reported type that inhabits the Kairei field, and both types of 'scaly-foot' gastropods genetically belong to the same species according to analyses of their COI gene and nuclear SSU rRNA gene sequences. However, the new morphotype completely lacks an iron-sulfide coating on the sclerites, which had been believed to be a novel feature restricted to 'scaly-foot' gastropods. Our new findings at the two newly discovered hydrothermal vent sites provide important insights into the biodiversity and biogeography of vent-endemic ecosystems in the Indian Ocean.

  10. Modeling hydrothermal processes at ocean spreading centers: Magma to microbe—An overview

    Science.gov (United States)

    Lowell, Robert P.; Seewald, Jeffrey S.; Metaxas, Anna; Perfit, Michael R.

    Hydrothermal processes at oceanic spreading centers encompass a number of highly interconnected processes ranging from the transport of mantle melts beneath spreading centers to the evolution of ocean chemistry and Earth's climate. This volume, which stems from a RIDGE Theoretical Institute held at Mammoth Lakes, California in June 2006, contains papers that address the complex connections among magmatic heat supply, crustal formation, seismicity, and hydrothermal circulation as well as the complex linkages among hydrothermal circulation, vent chemistry, carbon cycling, and microbial and macrofaunal ecosystems. The last paper in this volume explores the connection between hydrothermal venting and the chemical evolution of the oceans during the Phanerozoic. From reading these papers, one should recognize the wide variety of modeling approaches used and the uneven state of model development within various subdisciplines. Models of hydrothermal circulation and vent chemistry tend to be more quantitative, whereas models of carbon cycling and biological processes tend to be more conceptual. Although many of the complex linkages among the subdisciplines are understood at a conceptual level, considerable effort must be undertaken to develop integrated quantitative models of hydrothermal processes at oceanic spreading centers.

  11. Submarine Hydrothermal Systems - No Two Fields Are Alike

    Science.gov (United States)

    Kelley, D. S.

    2014-12-01

    Over 300 hydrothermal systems have been discovered since the first finding of Galapagos vents over three decades ago. The size, morphology, chemistry and associated biology show a rich diversity that is in part governed by their host rocks and tectonic setting. Each vent system is unique in terms of the morphology of black smoker edifices and associated diffuse flow, which suggests that local processes and feedback loops govern the nature and evolution of these dynamic systems. In fast-spreading environments (e.g. EPR), vent fields are spaced far apart and individual structures are small in number and size. In contrast, to date, the highest concentrations of fields per kilometer of ridge segment, and the largest individual black smokers occur in intermediate-spreading systems (e.g. Endeavour hosting 45 m-tall chimneys). The largest complexes occur in intermediate and slow-spreading environments (e.g. TAG at 200 m across). The highest temperature vents are transient, with temperature excursions at or above the critical point of seawater. Extremely high temperatures are associated with diking and eruptive events that likely vaporize subsurface fluids, forcing them across the two-phase boundary briefly. Along slow- and ultraslow-spreading ridges, the character of vents is strongly controlled by faulting, in particular, long-lived detachment faults that expose variably deformed and altered ultramafic rocks. Here, vent systems evolve from high-temperature black smokers within the axial valley with fluids rich in CO2, to black smokers with mantle and basaltic signatures along the axial valley walls, to end member systems such as the Lost City Field with chimneys and fluid chemistries never before seen: 60 m tall limestone towers that vent 90°C, metal-poor, pH 9-11 fluids devoid of CO2, yet rich in H2, CH4 and other low molecular weight hydrocarbons formed abiotically. This relatively stable environment, free from volcanic events, promotes venting for >150,000 years.

  12. Microbiological production and ecological flux of northwestern subduction hydrothermal systems

    Science.gov (United States)

    Sunamura, M.; Okamura, K.; Noguchi, T.; Yamamoto, H.; Fukuba, T.; Yanagawa, K.

    2012-12-01

    Deep-sea hydrothermal system is one of the most important sources for heat and chemical flux from the oceanic crust to the global ocean. The rich biological community around the hydrothermal vent shows chemolithoautotrophic microbial production are important in deep sea ecosystems. More than 99% of microbiological available chemical components in hydrothermal vent fluid, e.g. sulfide, methane, hydrogen, Fe2+, and Mn2+, is released into surrounding seawater to construct hydrothermal plume, suggesting that the chemolithoautotrophic-microbial primary production in the hydrothermal plume is huge and important in the whole hydrothermal ecosystems. To understand the impact of hydrothermal plume to a microbial ecosystem and a connectivity with zooplankton, we targeted and investigated a total of 16 hydrothermal fileds (7 sites in Okinawa trough, 3 sites in Ogasawara arc, and 6 sites in Mariana arc and back arc) and investigated in several cruises under the TAIGA project in Japan. Hydrothermal fluids in the subduction system are rich in sulfide. The hydrothermal fluids in the Okinawa trough, Ogasawara arc. and Mariana trough are characterized by rich in methane, poor in other reduced chemicals, and rich in iron, respectively. The major microbial composition was a potential sulfur oxidizing microbes SUP05 in the plume ecosystems, while an aerobic methanotrophic bacteria was secondary major member in methane-rich hydrothermal systems in Okinawa trough. Microbial quantitative and spatial distribution analyses of each plume site showed that the microbial population size and community structures are influenced by original chemical components of hydrothermal fluid, e.g. sulfide, methane and iron concentration. Microbial quantitative data indicated the removal/sedimentation of microbial cells from the plume and effect of phase separation in a same vent field through construction of gas-rich or gas-poor plumes. After the correlation of plume mixing effect, we estimates that the

  13. Time-series observations of hydrothermal discharge using an acoustic imaging sonar: a NEPTUNE observatory case study

    Science.gov (United States)

    Xu, Guangyu; Bemis, Karen; Jackson, Darrell; Light, Russ

    2015-04-01

    One intriguing feature of a mid-ocean ridge hydrothermal system is the intimate interconnections among hydrothermal, geological, oceanic, and biological processes. The advent of the NEPTUNE observatory operated by Ocean Networks Canada at the Endeavour Segment, Juan de Fuca Ridge enables scientists to study these interconnections through multidisciplinary, continuous, real-time observations. In this study, we present the time-series observations of a seafloor hydrothermal vent made using the Cabled Observatory Vent Imaging Sonar (COVIS). COVIS is currently connected to the NEPTUNE observatory to monitor the hydrothermal discharge from the Grotto mound on the Endeavour Segment. Since its deployment in 2010, COVIS has recorded a 3-year long dataset of the shape and outflow fluxes of the buoyant plumes above Grotto along with the areal coverage of its diffuse flow discharge. The interpretation of these data in light of contemporaneous observations of ocean currents, venting temperature, and seismicity made using other NEPTUNE observatory instruments reveals significant impacts of ocean currents and geological events on hydrothermal venting. In this study, we summarize these findings in the hope of forming a more complete understanding of the intricate interconnections among oceanic, geological, and hydrothermal processes.

  14. Geochemical constraints on chemolithoautotrophic metabolism by microorganisms in seafloor hydrothermal systems

    Science.gov (United States)

    McCollom, Thomas M.; Shock, Everett L.

    1997-10-01

    Mixing of hydrothermal fluids and seawater at the ocean floor, combined with slow reaction kinetics for oxidation/reduction reactions, provides a source of metabolic energy for chemolithotrophic microorganisms which are the primary biomass producers for an extensive submarine ecosystem that is essentially independent of photosynthesis. Thermodynamic models are used to explore geochemical constraints on the amount of metabolic energy potentially available from chemosynthetic reactions involving S, C, Fe, and Mn compounds during mixing of hydrothermal fluids with seawater. For the vent fluid used in the calculations (EPR 21°N OBS), the model indicates that mixing environments are favorable for oxidation of H 2S, CH 4, Fe 2+ and Mn 2+ only below ˜ 38°C, with methanogenesis and reduction of sulfate or S° favored at higher temperatures, suggesting that environments dominated by mixing provide habitats for mesophilic (but not thermophilic) aerobes and thermophilic (but not mesophilic) anaerobes. A maximum of ˜760 cal per kilogram vent fluid is available from sulfide oxidation while between 8 and 35 cal/kg vent fluid is available from methanotrophy, methanogenesis, oxidation of Fe or Mn, or sulfate reduction. The total potential for chemosynthetic primary production at deep-sea hydrothermal vents globally is estimated to be about 10 13 g biomass per year, which represents ˜0.02% of the global primary production by photosynthesis in the oceans. Thermophilic methanogens and sulfate- and S°-reducers are likely to be the predominant organisms in the walls of vent chimneys and in the diffuse mixing zones beneath warm vents, where biological processes may contribute to the high methane concentrations of vent fluids and heavy 34S/32S S ratios of vent sulfide minerals. The metabolic processes taking place in these systems may be analogs of the first living systems to evolve on the Earth.

  15. Modeling mid-ocean ridge hydrothermal response to earthquakes, tides, and ocean currents: a case study at the Grotto mound, Endeavour Segment, Juan de Fuca Ridge

    Science.gov (United States)

    Xu, G.; Bemis, K. G.

    2014-12-01

    Seafloor hydrothermal systems feature intricate interconnections among oceanic, geological, hydrothermal, and biological processes. The advent of the NEPTUNE observatory operated by Ocean Networks Canada at the Endeavour Segment, Juan de Fuca Ridge enables scientists to study these interconnections through multidisciplinary, continuous, real-time observations. The multidisciplinary observatory instruments deployed at the Grotto Mound, a major study site of the NEPTUNE observatory, makes it a perfect place to study the response of a seafloor hydrothermal system to geological and oceanic processes. In this study, we use the multidisciplinary datasets recorded by the NEPTUNE Observatory instruments as observational tools to demonstrate two different aspects of the response of hydrothermal activity at the Grotto Mound to geological and oceanic processes. First, we investigate a recent increase in venting temperature and heat flux at Grotto observed by the Benthic and Resistivity Sensors (BARS) and the Cabled Observatory Vent Imaging Sonar (COVIS) respectively. This event started in Mar 2014 and is still evolving by the time of writing this abstract. An initial interpretation in light of the seismic data recorded by a neighboring ocean bottom seismometer on the NEPTUNE observatory suggests the temperature and heat flux increase is probably triggered by local seismic activities. Comparison of the observations with the results of a 1-D mathematical model simulation of hydrothermal sub-seafloor circulation elucidates the potential mechanisms underlying hydrothermal response to local earthquakes. Second, we observe significant tidal oscillations in the venting temperature time series recorded by BARS and the acoustic imaging of hydrothermal plumes by COVIS, which is evidence for hydrothermal response to ocean tides and currents. We interpret the tidal oscillations of venting temperature as a result of tidal loading on a poroelastic medium. We then invoke poroelastic

  16. Formic acid production from carbohydrates biomass by hydrothermal reaction

    International Nuclear Information System (INIS)

    The formation of formic acid or formate salts by hydrothermal oxidation of model biomass materials (glucose, starch and cellulose) was investigated. All experiments were conducted in a batch reactor, made of SUS 316 tubing, providing an internal volume of 5.7 cm3. A 30 wt% hydrogen peroxide aqueous solution was used as an oxidant. The experiments were carried out with temperature of 2500C, reaction time varying from 0.5 min to 5 min, H2O2 supply of 240%, and alkaline concentration varying from 0 to 1.25 M. Similar to glucose, in the cases of the oxidation of hydrothermal starch and cellulose, the addition of alkaline can also improve the yield of formic acid. And the yield were glucose>starch> cellulose in cases of with or without of alkaline addition.

  17. The importance of shallow hydrothermal island arc systems in ocean biogeochemistry

    OpenAIRE

    Hawkes, Jeffrey A.; Connelly, Douglas P.; Rijkenberg, Micha J. A.; Achterberg, Eric P.

    2014-01-01

    Hydrothermal venting often occurs at submarine volcanic calderas on island arc chains, typically at shallower depths than mid-ocean ridges. The effect of these systems on ocean biogeochemistry has been under-investigated to date. Here we show that hydrothermal effluent from an island arc caldera was rich in Fe(III) colloids (0.02-0.2 mu m; 46% of total Fe), contributing to a fraction of hydrothermal Fe that was stable in ocean water. Iron(III) colloids from island arc calderas may be transfer...

  18. Biosphere in 3.5 Ga submarine hydrothermal system

    International Nuclear Information System (INIS)

    Abundant organic matter (kerogen) was identified in ∼3.5 Ga hydrothermal silica dikes from the North Pole area in the Pilbara craton, Western Australia. The silica dikes developed in the uppermost 1000 m of the ancient oceanic crust. Thus, they would have been deposited in the 3.5 Ga sub-seafloor hydrothermal system. The carbon and nitrogen isotopic compositions of the kerogen were analyzed in this study. Their highly 13C-depleted isotopic compositions (δ13C = -38 to -33 per mille) strongly suggest that they are originally derived from biologically produced organic matter. The remarkable similarity of the δ13C values between the kerogen and modern hydrothermal vent organisms may suggest that the kerogen was derived from chemoautotrophic organisms. This idea is also consistent with their nitrogen isotopic compositions (δ15N = -4 to +4 per mille). The silica dikes consist mainly of fine-grained silica with minor pyrite and sphalerite. These mineral assemblages indicate that the silica dike was deposited from relatively low-temperature (probably less than 150degC) reducing hydrothermal fluid. Thus, anaerobic thermophilic/hyperthermophilic organisms could have survived in the hydrothermal fluid, which formed the silica dikes. Therefore, it is plausible that a chemoautotrophic-based biosphere (possibly methanogenesis) probably existed in the Early Archean sub-seafloor hydrothermal system. (author)

  19. Extraordinary phase separation and segregation in vent fluids from the southern East Pacific Rise

    Science.gov (United States)

    Von Damm, K. L.; Lilley, M.D.; Shanks, Wayne C.; Brockington, M.; Bray, A.M.; O'Grady, K. M.; Olson, E.; Graham, A.; Proskurowski, G.

    2003-01-01

    The discovery of Brandon vent on the southern East Pacific Rise is providing new insights into the controls on midocean ridge hydrothermal vent fluid chemistry. The physical conditions at the time ofsampling (287 bar and 405??C) place the Brandon fluids very close to the critical point of seawater (298 bar and 407??C). This permits in situ study of the effects of near criticalphenomena, which are interpreted to be the primary cause of enhanced transition metal transport in these fluids. Of the five orifices on Brandon sampled, three were venting fluids with less than seawater chlorinity, and two were venting fluids with greater than seawater chlorinity. The liquid phase orifices contain 1.6-1.9 times the chloride content of the vapors. Most other elements, excluding the gases, have this same ratio demonstrating the conservative nature of phase separation and the lack of subsequent water-rock interaction. The vapor and liquid phases vent at the same time from orifices within meters of each other on the Brandon structure. Variations in fluid compositions occur on a time scale of minutes. Our interpretation is that phase separation and segregation must be occurring 'real time' within the sulfide structure itself. Fluids from Brandon therefore provide an unique opportunity to understand in situ phase separation without the overprinting of continued water-rock interaction with the oceanic crust, as well as critical phenomena. ?? 2002 Elsevier Science B.V. All rights reserved.

  20. Les Ventes-de-Bourse

    OpenAIRE

    Besnard, Michel; Noël, Jean-Yves; Nové-Josserand, Jacques; Fromont, Nicolas

    2013-01-01

    Date de l'opération : 2007 (EX) Inventeur(s) : Fromont Nicolas (INRAP) Le diagnostic archéologique mené au logis des Ventes aux Ventes-de-Bourse, commune du sud de l’Orne, a permis d’observer une surface d’un peu moins de 24 ha, située en rive gauche de la Vézone, affluent de la Sarthe. La topographie des parcelles concernées est relativement plane ; elles prennent place sur un aplat perché quelques mètres seulement au-dessus de ces rivières. En outre, le substratum, constitué d’assises argil...

  1. Investigating the active hydrothermal field of Kolumbo Volcano using CTD profiling

    Science.gov (United States)

    Eleni Christopoulou, Maria; Mertzimekis, Theo; Nomikou, Paraskevi; Papanikolaou, Dimitrios; Carey, Steve

    2014-05-01

    The submarine Kolumbo volcano NE of Santorini Island and the unique active hydrothermal vent field on its crater field (depth ~ 500 m) have been recently explored in multiple cruises aboard E/V Nautilus. ROV explorations showed the existence of extensive vent activity and almost completely absence of vent-specific macrofauna. Gas discharges have been found to be 99%-rich in CO2, which is sequestered at the bottom of the crater due to a special combination of physicochemical and geomorphological factors. The dynamic conditions existing along the water column in the crater have been studied in detail by means of temperature, salinity and conductivity depth profiles for the first time. CTD sensors aboard the ROV Hercules were employed to record anomalies in those parameters in an attempt to investigate several active and inactive vent locations. Temporal CTD monitoring inside and outside of the crater was carried out over a period of two years. Direct comparison between the vent field and locations outside the main cone, where no hydrothermal activity is known to exist, showed completely different characteristics. CTD profiles above the active vent field (NNE side) are correlated to Kolumbo's cone morphology. The profiles suggest the existence of four distinct zones of physicochemical properties in the water column. The layer directly above the chimneys exhibit gas discharges highly enriched in CO2. Continuous gas motoring is essential to identify the onset of geological hazards in the region.

  2. Age, Episodicity and Migration of Hydrothermal Activity within the Axial Valley, Endeavour Segment, Juan de Fuca Ridge

    Science.gov (United States)

    Jamieson, J. W.; Hannington, M. D.; Kelley, D. S.; Clague, D. A.; Holden, J. F.; Tivey, M. K.; Delaney, J. R.

    2011-12-01

    Hydrothermal sulfide deposits record the history of high-temperature venting along the Endeavour Segment. Active venting is currently located within five discreet vent fields, with minor diffuse venting occurring between the fields. However, inactive and/or extinct sulfide structures are found throughout the entire axial valley of the ridge segment, suggesting that hydrothermal activity has been more vigorous in the past or focused venting has migrated with time. Here, we present age constraints from U-series dating of 44 sulfide samples collected by manned submersible from between the Mothra Field in the south to Sasquatch in the north. Samples are dated using 226Ra/Ba ratios from hydrothermal barite that precipitates along with the sulfide minerals. Most samples have been collected from within or near the active vent fields. Fifteen samples from the Main Endeavour Field (MEF) show a spectrum of ages from present to 2,430 years old, indicating that this field has been continuously active for at least ~2,400 years. MEF appears to be oldest currently active field. This minimum value for the age of hydrothermal activity also provides a minimum age of the axial valley itself. Ages from thirteen samples from the High-Rise Field indicate continuous venting for at least the past ~1,250 years. These age data are used in conjunction with age constraints of the volcanic flows to develop an integrated volcanic, hydrothermal and tectonic history of the Endeavour Segment. The total volume of hydrothermal sulfide within the axial valley, determined from high-resolution bathymetry, is used in conjunction with the age constraints of the sulfide material to determine the mass accumulation rates of sulfide along the Endeavour Segment. These data can be used to calibrate the efficiency of sulfide deposition from the hydrothermal vents, and provide a time-integrated history of heat, fluid and chemical fluxes at the ridge-segment scale. The comparison of time-integrated rates with

  3. Partitioning Between Plume and Diffuse Flow at the Grotto Vent Cluster, Main Endeavour Vent Field, Juan de Fuca Ridge: Past and Present

    Science.gov (United States)

    Rona, P. A.; Bemis, K. G.; Jones, C.; Jackson, D. R.; Mitsuzawa, K.; Palmer, D. R.

    2010-12-01

    Seafloor hydrothermal systems discharge as plumes from discrete vents and as diffuse flow from surrounding areas that transfer heat and chemicals from the lithosphere into the ocean in quantitatively significant amounts. Our VIP (Vent Imaging Pacific) July 2000 experiment was designed to acoustically image and quantify these flows at vent clusters in the Main Endeavour field using a sonar system (Simrad SM 2000) mounted on ROV Jason from fixed positions on the seafloor using three methods that we developed. Buoyant plumes are reconstructed applying visualization techniques to volume backscatter from suspended particulates and density discontinuities in plumes. A Doppler algorithm is used to measure mean vertical velocity through plume cross sections and to calculate volume flux at different altitudes in a plume. Acoustic Scintillation Thermography (AST) is used to image irregular areas of diffuse flow. Results from a sulfide edifice (north tower of Grotto Vent cluster; height 12 m; diameter 10 m; sonar range 12 m) supplemented by additional AST measurements and in situ measurements of flow rate and temperature are tabulatd below: The higher diffuse than plume heat flux is consistent with prior studies at other sites. However, the ratio of diffuse to plume heat flux (range 23-353) is exceptionally large suggesting an overestimation of diffuse flow area at the high end and/or underestimation of plume flux. We are scheduled this fall to connect our next generation sonar system (COVIS=Cable Operated Vent Imaging Sonar) to the NEPTUNE Canada cabled observatory at the Grotto vent cluster. COVIS is designed to image plume and diffuse flow in space and in time, which will contribute to interpreting our past observations and to provide new insights on how partitioning may vary with time.

  4. Mineralogy at the magma-hydrothermal system interface in andesite volcanoes, New Zealand

    Science.gov (United States)

    Wood, C. Peter

    1994-01-01

    Ejecta from phreatomagmatic eruptions of Ruapehu and White Island andesite volcanoes in New Zealand provide insight into the mineralogical reactions that occur when magma invades a vent-hosted hydrothermal system. At the surface and in ejected blocks from shallow depths, hydrothermal alteration mineralogies are dominated by silica polymorphs, anhydrite, natroalunite, and pyrite. Blocks from greater depths are composed mainly of cristobalite, anhydrite, halite, and magnetite. Where altered material was heated to magmatic temperatures, thermal decomposition reactions produced mullite, wollastonite, and indialite. Some ejected breccias contain osumilite, cordierite, sanidine, and hypersthene, indicative of reactions occurring near the osumilite-cordierite phase boundary at >800 °C and water pressure hydrothermal deposits. High- temperature parageneses of these types have not been reported before in shallow, acidic volcano-hydrothermal systems. However, they may be typical of the magma- hydrothermal contact zone at many andesite volcanoes.

  5. PZT Powders Synthesized by Hydrothermal Method

    Directory of Open Access Journals (Sweden)

    ZHU Kong-Jun, ZHU Ren-Qiang, DONG Na-Na, GU Hong-Hui, QIU Jin-Hao, JI Hong-Li

    2012-05-01

    Full Text Available Lead zirconate titanate (PZT) powders with single―phase, cubic morphology and average size of 1 μm were synthesized using hydrothermal methods. Effects of KOH concentration, hydrothermal treatment time and Pb excess were investigated. The results show that KOH concentration has an important influence on the solid solubility of Pb ion in A―site of PZT perovskite structure. The concentration of Pb2+ vacancies increased with the increase of OH― concentration. However, this kind of deficiency could be compensated by adding more Pb ions in the raw materials, and more Pb ions were needed to add with higher alkaline concentration in the starting solution. But excessive compensation of Pb ions would result in the appearance of the second phase.

  6. Hydrothermal alteration of sediments associated with surface emissions from the Cerro Prieto geothermal field

    Energy Technology Data Exchange (ETDEWEB)

    Valette-Silver, J.N.; Esquer P., I.; Elders, W.A.; Collier, P.C.; Hoagland, J.R.

    1981-01-01

    A study of the mineralogical changes associated with these hydrothermal vents was initiated with the aim of developing possible exploration tools for geothermal resources. The Cerro Prieto reservoir has already been explored by extensive deep drilling so that relationships between surface manifestations and deeper hydrothermal processes could be established directly. Approximately 120 samples of surface sediments were collected both inside and outside of the vents. The mineralogy of the altered sediments studied appears to be controlled by the type of emission. A comparison between the changes in mineralogy due to low temperature hydrothermal activity in the reservoir, seen in samples from boreholes, and mineralogical changes in the surface emission samples shows similar general trends below 180 C: increase of quartz, feldspar and illite, with subsequent disappearance of kaolinite, montmorillonite, calcite and dolomite. These mineral assemblages seem to be characteristic products of the discharge from high intensity geothermal fields.

  7. Serpentinization-assisted deformation processes and characterization of hydrothermal fluxes at mid-ocean ridges

    Science.gov (United States)

    Genc, Gence

    Seafloor hydrothermal systems play a significantly important role in Earth’s energy and geochemical budgets and support the existence and development of complex biological ecosystems by providing nutrient and energy to microbial and macrafaunal ecosystems through geochemical fluxes. Heat output and fluid flow are key parameters which characterize hydrothermal systems at oceanic spreading centers by constraining models of hydrothermal circulation. Although integrated measurements of heat flux in plumes are critically important as well, quantification of heat flux at discrete sources (vent orifices versus patches of seafloor shimmering diffuse flow) from direct measurements is particularly essential for examining the partitioning of heat flow into focused and diffuse components of venting and determining geochemical fluxes from these two modes of flow. Hydrothermal heat output also constrains the permeability of young oceanic crust and thickness of the conductive boundary layer that separates magmatic heat source from overlying hydrothermal circulation. This dissertation will be fundamentally focused on three main inter-connected topics: (1) the design and development of direct high- or low-temperature heat flow measuring devices for hydrothermal systems, (2) the collection of new heat output results on four cruises between 2008 and 2010 at several distinct hydrothermal sites along mid-ocean ridges (MORs) to estimate total heat output from individual vent structures such as Dante, Hulk or the whole vent field (e.g., Main Endeavour Vent Field (MEF)), the partitioning between focused and diffuse hydrothermal venting in MEF, and determination of initial estimates of geochemical flux from diffuse hydrothermal fluids which may be influenced by the activity in subsurface biosphere and finally (3) the deformation and uplift associated with serpentinization at MORs and subduction zones. Despite extensive efforts spent for the last couple of decades on heat flow measurement

  8. Tide-related variability of TAG hydrothermal activity observed by deep-sea monitoring system and OBSH

    Science.gov (United States)

    Fujioka, Kantaro; Kobayashi, Kazuo; Kato, Kazuhiro; Aoki, Misumi; Mitsuzawa, Kyohiko; Kinoshita, Masataka; Nishizawa, Azusa

    1997-12-01

    Hydrothermal activities were monitored by an ocean bottom seismometer with hydrophone (OBSH) and a composite measuring system (Manatee) including CTD, current meter, transmission meter and cameras at a small depression on the TAG hydrothermal mound in the Mid-Atlantic Ridge. Low-frequency pressure pulses detected by the hydrophone with semi-diurnal periodicity seem to correspond to cycles of hydrothermal upflow from a small and short-lived smoker vent close to the observing site. The peaks of pressure pulses are synchronous with the maximum gradient of areal strain decrease due to tidal load release. Microearthquakes with very near epicenters occur sporadically and do not appear to be directly correlatable to hydrothermal venting. Temporal variations in bottom water temperature also have semi-diurnal periodicity but are more complicated than the pressure events. Temperatures may be affected both by upwelling of hot water and by lateral flow of the bottom current changing its directions with ocean tide.

  9. Hydrothermal Events on Hypercard.

    Science.gov (United States)

    Glickstein, Neil

    1997-01-01

    Explains how students developed Hypercard stacks to report the results of their study of vent science. Describes each step in the project process that included projects related to geography, technology, physics, chemistry, and biology. (DDR)

  10. Anodes for alkaline electrolysis

    Science.gov (United States)

    Soloveichik, Grigorii Lev

    2011-02-01

    A method of making an anode for alkaline electrolysis cells includes adsorption of precursor material on a carbonaceous material, conversion of the precursor material to hydroxide form and conversion of precursor material from hydroxide form to oxy-hydroxide form within the alkaline electrolysis cell.

  11. Alkaline "Permanent" Paper.

    Science.gov (United States)

    Pacey, Antony

    1991-01-01

    Discussion of paper manufacturing processes and their effects on library materials focuses on the promotion of alkaline "permanent" paper, with less acid, by Canadian library preservation specialists. Standards for paper acidity are explained; advantages of alkaline paper are described, including decreased manufacturing costs; and recyclability is…

  12. Dissolved organic carbon in ridge-axis and ridge-flank hydrothermal systems

    Science.gov (United States)

    Lang, Susan Q.; Butterfield, David A.; Lilley, Marvin D.; Paul Johnson, H.; Hedges, John I.

    2006-08-01

    The circulation of hydrothermal fluid through the upper oceanic crustal reservoir has a large impact on the chemistry of seawater, yet the impact on dissolved organic carbon (DOC) in the ocean has received almost no attention. To determine whether hydrothermal circulation is a source or a sink for DOC in the oceans, we measured DOC concentrations in hydrothermal fluids from several environments. Hydrothermal fluids were collected from high-temperature vents and diffuse, low-temperature vents on the basalt-hosted Juan de Fuca Ridge axis and also from low-temperature vents on the sedimented eastern flanks. High-temperature fluids from Main Endeavour Field (MEF) and Axial Volcano (AV) contain very low DOC concentrations (average = 15 and 17 μM, respectively) compared to background seawater (36 μM). At MEF and AV, average DOC concentrations in diffuse fluids (47 and 48 μM, respectively) were elevated over background seawater, and high DOC is correlated with high microbial cell counts in diffuse fluids. Fluids from off-axis hydrothermal systems located on 3.5-Ma-old crust at Baby Bare Seamount and Ocean Drilling Program (ODP) Hole 1026B had average DOC concentrations of 11 and 13 μM, respectively, and lowered DOC was correlated with low cell counts. The relative importance of heterotrophic uptake, abiotic sorption to mineral surfaces, thermal decomposition, and microbial production in fixing the DOC concentration in vent fluids remains uncertain. We calculated the potential effect of hydrothermal circulation on the deep-sea DOC cycle using our concentration data and published water flux estimates. Maximum calculated fluxes of DOC are minor compared to most oceanic DOC source and sink terms.

  13. Research related to filtered venting system

    International Nuclear Information System (INIS)

    This paper describes how to write a manuscript of the 18th PES Proceedings. In the Fukushima Daiichi Nuclear Power Plant accident, a great deal of radioactive material was released to surrounding environment. After the accident, the Filtered Containment Venting System (FCVS) that can remove radioactive material from steam in containment vessel has attracted a great deal of attentions. However, there is few knowledge of filtered vent in Japan because almost filtered vent were developed in Europe after Chernobyl nuclear power plant accident. Therefore, our purpose is getting the knowledge of filtered vent through experiments that simulate various filter vents in Europe. Also using the knowledge gotten through experiment, we aim at developing high efficiency filtered containment venting system using zeolite. (author)

  14. High-resolution near-bottom vector magnetic anomalies over Raven Hydrothermal Field, Endeavour Segment, Juan de Fuca Ridge

    Science.gov (United States)

    Tivey, Maurice A.; Johnson, H. Paul; Salmi, Marie S.; Hutnak, Michael

    2014-10-01

    High-resolution, near-bottom vector magnetic data were collected by remotely operated vehicle Jason over the Raven hydrothermal vent field (47°57.3'N 129°5.75'W) located north of Main Endeavour vent field on the Endeavour segment of the Juan de Fuca Ridge. The survey was part of a comprehensive heat flow study of the Raven site using innovative thermal blanket technology to map the heat flux and crustal fluid pathways around a solitary hydrothermal vent field. Raven hydrothermal activity is presently located along the western axial valley wall, while additional inactive hydrothermal deposits are found to the NW on the upper rift valley wall. Magnetic inversion results show discrete areas of reduced magnetization associated with both active and inactive hydrothermal vent deposits that also show high conductive heat flow. Higher spatial variability in the heat flow patterns compared to the magnetization is consistent with the heat flow reflecting the currently active but ephemeral thermal environment of fluid flow, while crustal magnetization is representative of the static time-averaged effect of hydrothermal alteration. A general NW to SE trend in reduced magnetization across the Raven area correlates closely with the distribution of hydrothermal deposits and heat flux patterns and suggests that the fluid circulation system at depth is likely controlled by local crustal structure and magma chamber geometry. Magnetic gradient tensor components computed from vector magnetic data improve the resolution of the magnetic anomaly source and indicate that the hydrothermally altered zone directly beneath the Raven site is approximately 15 × 106 m3 in volume.

  15. Summary of performance test for filtered vent

    International Nuclear Information System (INIS)

    TOSHIBA has been carried tests to evaluate performance of the filtered vent system. In order to apply several parameters and functions, test facility is useful and has much flexibility. The filtered vent system has 2 functions, one is scrubbing in water and the other is metal filter. Test results show a good performance. We will introduce a outline of the filtered vent system examination. (author)

  16. Parameterization of and Brine Storage in MOR Hydrothermal Systems

    Science.gov (United States)

    Hoover, J.; Lowell, R. P.; Cummings, K. B.

    2009-12-01

    Single-pass parameterized models of high-temperature hydrothermal systems at oceanic spreading centers use observational constraints such as vent temperature, heat output, vent field area, and the area of heat extraction from the sub-axial magma chamber to deduce fundamental hydrothermal parameters such as total mass flux Q, bulk permeability k, and the thickness of the conductive boundary layer at the base of the system, δ. Of the more than 300 known systems, constraining data are available for less than 10%. Here we use the single pass model to estimate Q, k, and δ for all the seafloor hydrothermal systems for which the constraining data are available. Mean values of Q, k, and δ are 170 kg/s, 5.0x10-13 m2, and 20 m, respectively; which is similar to results obtained from the generic model. There is no apparent correlation with spreading rate. Using observed vent field lifetimes, the rate of magma replenishment can also be calculated. Essentially all high-temperature hydrothermal systems at oceanic spreading centers undergo phase separation, yielding a low chlorinity vapor and a high salinity brine. Some systems such as the Main Endeavour Field on the Juan de Fuca Ridge and the 9°50’N sites on the East Pacific Rise vent low chlorinity vapor for many years, while the high density brine remains sequestered beneath the seafloor. In an attempt to further understand the brine storage at the EPR, we used the mass flux Q determined above, time series of vent salinity and temperature, and the depth of the magma chamber to determine the rate of brine production at depth. We found thicknesses ranging from 0.32 meters to ~57 meters over a 1 km2 area from 1994-2002. These calculations suggest that brine maybe being stored within the conductive boundary layer without a need for lateral transport or removal by other means. We plan to use the numerical code FISHES to further test this idea.

  17. Explosive Volcanic Eruptions from Linear Vents on Earth, Venus and Mars: Comparisons with Circular Vent Eruptions

    Science.gov (United States)

    Glaze, Lori S.; Baloga, Stephen M.; Wimert, Jesse

    2010-01-01

    Conditions required to support buoyant convective plumes are investigated for explosive volcanic eruptions from circular and linear vents on Earth, Venus, and Mars. Vent geometry (linear versus circular) plays a significant role in the ability of an explosive eruption to sustain a buoyant plume. On Earth, linear and circular vent eruptions are both capable of driving buoyant plumes to equivalent maximum rise heights, however, linear vent plumes are more sensitive to vent size. For analogous mass eruption rates, linear vent plumes surpass circular vent plumes in entrainment efficiency approximately when L(sub o) > 3r(sub o) owing to the larger entrainment area relative to the control volume. Relative to circular vents, linear vents on Venus favor column collapse and the formation of pyroclastic flows because the range of conditions required to establish and sustain buoyancy is narrow. When buoyancy can be sustained, however, maximum plume heights exceed those from circular vents. For current atmospheric conditions on Mars, linear vent eruptions are capable of injecting volcanic material slightly higher than analogous circular vent eruptions. However, both geometries are more likely to produce pyroclastic fountains, as opposed to convective plumes, owing to the low density atmosphere. Due to the atmospheric density profile and water content on Earth, explosive eruptions enjoy favorable conditions for producing sustained buoyant columns, while pyroclastic flows would be relatively more prevalent on Venus and Mars. These results have implications for the injection and dispersal of particulates into the planetary atmosphere and the ability to interpret the geologic record of planetary volcanism.

  18. Alkaline battery operational methodology

    Energy Technology Data Exchange (ETDEWEB)

    Sholklapper, Tal; Gallaway, Joshua; Steingart, Daniel; Ingale, Nilesh; Nyce, Michael

    2016-08-16

    Methods of using specific operational charge and discharge parameters to extend the life of alkaline batteries are disclosed. The methods can be used with any commercial primary or secondary alkaline battery, as well as with newer alkaline battery designs, including batteries with flowing electrolyte. The methods include cycling batteries within a narrow operating voltage window, with minimum and maximum cut-off voltages that are set based on battery characteristics and environmental conditions. The narrow voltage window decreases available capacity but allows the batteries to be cycled for hundreds or thousands of times.

  19. DESIGN OF NOVEL HIGH PRESSURE- RESISTANT HYDROTHERMAL FLUID SAMPLE VALVE

    Institute of Scientific and Technical Information of China (English)

    LIU Wei; YANG Canjun; WU Shijun; XIE Yingjun; CHEN Ying

    2008-01-01

    Sampling study is an effective exploration method, but the most extreme environments of hydrothermal vents pose considerable engineering challenges for sampling hydrothermal fluids. Moreover, traditional sampler systems with sample valves have difficulty in maintaining samples in situ pressure. However, decompression changes have effect on microorganisms sensitive to such stresses. To address the technical difficulty of collecting samples from hydrothermal vents, a new bidirectional high pressure-resistant sample valve with balanced poppet was designed. The sample valve utilizes a soft high performance plastic "PEEK" as poppet. The poppet with inapposite dimension is prone to occur to plastic deformation or rupture for high working pressure in experiments. To address this issue, based on the finite element model, simulated results on stress distribution of the poppet with different structure parameters and preload spring force were obtained. The static axial deformations on top of the poppet were experimented. The simulated results agree with the experimental results. The new sample valve seals well and it can withstand high working pressure.

  20. Talc-dominated seafloor deposits reveal a new class of hydrothermal system

    Science.gov (United States)

    Hodgkinson, Matthew R. S.; Webber, Alexander P.; Roberts, Stephen; Mills, Rachel A.; Connelly, Douglas P.; Murton, Bramley J.

    2015-12-01

    The Von Damm Vent Field (VDVF) is located on the flanks of the Mid-Cayman Spreading Centre, 13 km west of the axial rift, within a gabbro and peridotite basement. Unlike any other active vent field, hydrothermal precipitates at the VDVF comprise 85-90% by volume of the magnesium silicate mineral, talc. Hydrothermal fluids vent from a 3-m high, 1-m diameter chimney and other orifices at up to 215 °C with low metal concentrations, intermediate pH (5.8) and high concentrations (667 mmol kg-1) of chloride relative to seawater. Here we show that the VDVF vent fluid is generated by interaction of seawater with a mafic and ultramafic basement which precipitates talc on mixing with seawater. The heat flux at the VDVF is measured at 487+/-101 MW, comparable to the most powerful magma-driven hydrothermal systems known, and may represent a significant mode of off-axis oceanic crustal cooling not previously recognized or accounted for in global models.

  1. Early-stage mineralization of hydrothermal tubeworms: New insights into the role of microorganisms in theprocess of mineralization

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    As an important part of marine hydrothermal system, hydrothermal vent faunas live in hydrothermal inorganic environment and closely interact with hydrothermal inorganic environment. Sometimes, they can participate in the mineralization process of modern hydrothermal site. Hydrothermal vent faunas,particularly vestimentiferan and polychaete tubeworms, are occasionally preserved in the geological record. Study on the early mineralization process of hydrothermal vent fauna is significant for understanding the interaction between mineral and organism, and also the formation and preservation mechanism of geological fossil in hydrothermal environment. In this paper, the early stage of mineralization of Vestimentiferan Ridgeia piscesae tubes collected from Juan de Fuca Ridge is studied. The results showed that a lot of filamentous microorganisms were unevenly distributed on the surface of internal wall and in the interspace of the wall of tubeworm. In some cases, microorganisms aggregated as thin layers in or on the wall of tubeworm. The surfaces of microbial cells and the products of microbial degradation may play an important role in the early mineralization of tubeworm. Semitransparent thin layers of organic matter containing sulfur and sulfur granules were commonly found on the wall of tubeworm with lower degree of mineralization. The degradation production of these semitransparent thin layers may accelerate the mineralization of tube wall during the early stage. EDS results showed that on the tube walls some chemical elements such as Fe, P, Ca and Si are selectively enriched from ambient hydrothermal environment. Interestingly, P, Ca and Si covary with Fe content. Because element S originated from the bio-oxidation of H2S by symbiotic microorganism in the tissue of tubeworm, it can be considered as a biomarker when studying the mineralization process of tube wall. Based on the characteristics of tubeworms with different degrees of mineralization, we suggested that

  2. Uranium in alkaline rocks

    Energy Technology Data Exchange (ETDEWEB)

    Murphy, M.; Wollenberg, H.; Strisower, B.; Bowman, H.; Flexser, S.; Carmichael, I.

    1978-04-01

    Geologic and geochemical criteria were developed for the occurrence of economic uranium deposits in alkaline igneous rocks. A literature search, a limited chemical analytical program, and visits to three prominent alkaline-rock localities (Ilimaussaq, Greenland; Pocos de Caldas, Brazil; and Powderhorn, Colorado) were made to establish criteria to determine if a site had some uranium resource potential. From the literature, four alkaline-intrusive occurrences of differing character were identified as type-localities for uranium mineralization, and the important aspects of these localities were described. These characteristics were used to categorize and evaluate U.S. occurrences. The literature search disclosed 69 U.S. sites, encompassing nepheline syenite, alkaline granite, and carbonatite. It was possible to compare two-thirds of these sites to the type localities. A ranking system identified ten of the sites as most likely to have uranium resource potential.

  3. Uranium in alkaline rocks

    International Nuclear Information System (INIS)

    Geologic and geochemical criteria were developed for the occurrence of economic uranium deposits in alkaline igneous rocks. A literature search, a limited chemical analytical program, and visits to three prominent alkaline-rock localities (Ilimaussaq, Greenland; Pocos de Caldas, Brazil; and Powderhorn, Colorado) were made to establish criteria to determine if a site had some uranium resource potential. From the literature, four alkaline-intrusive occurrences of differing character were identified as type-localities for uranium mineralization, and the important aspects of these localities were described. These characteristics were used to categorize and evaluate U.S. occurrences. The literature search disclosed 69 U.S. sites, encompassing nepheline syenite, alkaline granite, and carbonatite. It was possible to compare two-thirds of these sites to the type localities. A ranking system identified ten of the sites as most likely to have uranium resource potential

  4. The vented pressure fed gas journal bearing

    International Nuclear Information System (INIS)

    Hydrodynamic-type gas journal bearings with stabilising venting slots are often operated hydrostatically during starting-up as a means of 'jacking'. A simplified mathematical treatment of the circumferential gas flows in a vented, pressure-fed journal bearing is used to predict the relationship between load capacity, bearing geometry and gas properties. (author)

  5. 46 CFR 98.25-70 - Venting.

    Science.gov (United States)

    2010-10-01

    ... the back pressure in relief valve discharge lines shall not be more than 10 percent of the safety relief valve setting. (d) Return bends and restrictive pipe fittings are prohibited. Vents and headers shall be so installed as to prevent stresses on safety relief valve mountings. (e) When vent...

  6. Review of containment vent filter technology

    International Nuclear Information System (INIS)

    The technology applied for the design and construction of containment vent filters is compiled and reviewed. The national positions leading to the selection of venting or method of filtration are extracted from position papers. Several areas of further information needs are identified

  7. Hydrothermal plumes in the NE Lau basin: A regional perspective

    Science.gov (United States)

    Walker, S. L.; Baker, E. T.

    2013-12-01

    Exploration for mineral resources and the presence of an extensive plume of excess 3He centered at 1750 m water depth in the Samoa-Tonga-Fiji region (Lupton, 2004) have motivated exploration for active hydrothermal vent sites in the NE Lau basin during the past decade. The region is tectonically complex with back-arc spreading centers, rift zones, and volcanic centers, all of which potentially host active venting and/or active volcanism. To date, 400 km of the three back-arc spreading centers in the NE Lau basin (FRSC, Fonualei Rift and Spreading Center; MTJ, Mangatolu Triple Junction; and NELSC, Northeastern Lau Spreading Center) plus several volcanic centers have been systematically surveyed for hydrothermal plumes using towed CTD or MAPR arrays that include both optical backscatter and oxidation-reduction potential (ORP) sensors. The FRSC, where spreading rates range from 47 mm/a in the south to 85 mm/a in the north, has 5 active sites (plume depths ranging from 1300-2200 m) distributed one every ~40 km over its 200 km length. There is evidence for 4 active sites (plume depths range from 1950-2380 m) along the 150 km combined length of the MTJ segments, however plumes were optically weak (dNTU < 0.02) and except for one location along the northeastern limb, no ORP anomalies were detected. Plumes were observed off-axis to the MTJ at a bathymetric high adjacent to the northeastern limb (1700 m) as well as over the summit of a cratered volcanic edifice east of the central junction (1200-1300 m). The southern segment of the NELSC was the site of an active eruption in 2008 which injected event plumes throughout the water column (900-1600 m depth range) in addition to the chronic plume from the Maka massive sulfide vent site (1500 m). There is evidence for at least two additional active areas along the northern segments of the NELSC (1800-1900 m). Several volcanoes in the region are hydrothermally active ranging from the northernmost volcano on the Tonga arc (Niua

  8. Particulate DNA in smoker fluids: Evidence for existence of microbial populations in hot hydrothermal systems

    Energy Technology Data Exchange (ETDEWEB)

    Straube, W.L.; Colwell, R.R. (Univ. of Maryland, College Park (USA) Univ. of Maryland, Baltimore (USA)); Deming, J.W.; Baross, J.A. (Univ. of Washington, Seattle (USA)); Somerville, C.C. (Univ. of Maryland, College Park (USA))

    1990-05-01

    As part of an interdisciplinary study of hydrothermal vents on the Endeavour Segment of the Juan de Fuca Ridge, we used the submersible ALVIN to collect 57 fluid samples from 17 different hot vents (smokers and flanges) and their environs for the purpose of extracting particulate DNA. Particulate material concentrated from these samples was lysed enzymatically (enz) and by a combination of enzyme and French press treatment (fp). Concentrations of partially purified DNA recovered from these lysates were determined spectrofluorometrically. Ambient seawater surrounding the vents was found to contain low DNA concentrations, 0.18 to 0.32 ng of DNA per ml, while low-temperature vent samples yielded significantly higher concentrations of 0.37 to 2.12 ng of DNA per ml. Although DNA recovery values from superheated (210 to 345{degree}C) flange samples were not significantly different from ambient seawater values, most of the superheated (174 to 357{degree}C) smoker fluid samples contained particulate DNA in concentrations too high to be attributable to entrained seawater. Detailed sampling at one smoker site demonstrated not only the existence of significant levels of particulate DNA in the superheated smoker fluids but also the presence of an elevated microbial population in the buoyant plume 20 to 100 m above the smoker. These results underscore the heterogeneity of smoker environments within a given hydrothermal vent fluid and indicate that microorganisms exist in some superheated fluids.

  9. Geology of a vigorous hydrothermal system on the Endeavour segment, Juan de Fuca Ridge

    Energy Technology Data Exchange (ETDEWEB)

    Delaney, J.R.; Robigou, V.; McDuff, R.E. (Univ. of Washington, Seattle (United States)); Tivey, M.K. (Woods Hole Oceanographic Institution, MA (United States))

    1992-12-10

    A high-precision, high-resolution geologic map explicitly documents relationships between tectonic features and large steep-sided, sulfide-sulfate-silica deposits in the vigorously venting Endeavour hydrothermal field near the northern end of the Juan de Fuca Ridge. Location of the most massive sulfide structures appears to be controlled by intersections of ridge-parallel normal faults and other fracture-fissure sets that trend oblique to, and perpendicular to the overall structural fabric of the axial valley. As presently mapped, the field is about 200 by 400 m on a side and contains at least 15 large (> 1,000 m[sup 3]) sulfide edifices and many tens of smaller, commonly inactive, sulfide structures. The larger sulfide structures are also the most vigorously venting features in the field; they are commonly more than 30 m in diameter and up to 20 m in height. Maximum venting temperatures of 375[degrees]C are associated with the smaller structures in the northern portion of the field are consistently 20[degrees]-30[degrees]C lower. Hydrothermal output from individual active sulfide features varies from no flow in the lower third of the edifice to vigorous output from fracture-controlled black smoker activity near the top of the structures. Two types of diffuse venting in the Endeavour field include a lower temperature 8[degrees]-15[degrees]C output through colonies of large tubeworms and 25[degrees]-50[degrees]C vent fluid that seems to percolate through the tops of overhanging flanges. The large size and steep-walled nature of these structures evidently results from sustained venting in a mature hydrothermal system, coupled with dual mineral depositional mechanisms involving vertical growth by accumulation of chimney sulfide debris and lateral growth by means of flange development.

  10. Hydrothermal mineralization at Kick'em Jenny submarine volcano in the Lesser Antilles island arc

    Science.gov (United States)

    Olsen, R.; Carey, S.; Sigurdsson, H.; Cornell, W. C.

    2011-12-01

    Kick 'em Jenny (KeJ) is an active submarine volcano located in the Lesser Antilles island arc, ~7.5 km northwest of Grenada. Of the twelve eruptions detected since 1939, most have been explosive as evidenced by eyewitness accounts in 1939, 1974, and 1988 and the dominance of explosive eruption products recovered by dredging. In 2003, vigorous hydrothermal activity was observed in the crater of KeJ. Video footage taken by a remotely operated vehicle (ROV) during the cruise RB-03-03 of the R/V Ronald Brown documented the venting of a vapor phase in the form of bubbles that ascended through the water column and a clear fluid phase in the form of shimmering water. The shimmering water generally ascended through the water column but can also been seen flowing down gradient from a fissure at the top of a fine-grained sediment mound. These fine-grained sediment mounds are the only structure associated with hydrothermal venting; spire or chimney structures were not observed. Hydrothermal venting was also observed coming from patches of coarse-grained volcaniclastic sediment on the crater floor and from talus slopes around the perimeter of the crater. Samples were collected from these areas and from areas void of hydrothermal activity. XRD and ICPMS analyses of bulk sediment were carried out to investigate the geochemical relationships between sediment types. Sediment samples from the hydrothermal mound structures are comprised of the same components (plagioclase, amphibole, pyroxene, and scoria) as sediment samples from areas void of hydrothermal activity (primary volcaniclastic sediment) in the 500-63 μm size range. High resolution grain size analyses show that >78% of sediment in the hydrothermal mound samples are between 63-2 μm with 6-20% clay sized (talc, and I/S mixed layer) in the hydrothermal mound samples was confirmed x-ray diffraction analysis. Differences in major oxide composition of the two sediment types (depletion in Al2O3 but enrichments in MgO and Fe2O3

  11. Uranium hydrothermal deposits

    Czech Academy of Sciences Publication Activity Database

    René, Miloš

    New York : Nova Science Publishers, 2012 - (Vasiliev, A.; Sidorov, M.), s. 211-244 ISBN 978-1-62081-207-5 Institutional research plan: CEZ:AV0Z30460519 Keywords : uranium * hydrothermal deposits * uraninite Subject RIV: DB - Geology ; Mineralogy

  12. Hydrothermal liquefaction of biomass

    DEFF Research Database (Denmark)

    Toor, Saqib; Rosendahl, Lasse; Rudolf, Andreas

    2011-01-01

    dehydration or decarboxylation. The chemical properties of bio-oil are highly dependent of the biomass substrate composition. Biomass constitutes of various components such as protein; carbohydrates, lignin and fat, and each of them produce distinct spectra of compounds during hydrothermal liquefaction. In......This article reviews the hydrothermal liquefaction of biomass with the aim of describing the current status of the technology. Hydrothermal liquefaction is a medium-temperature, high-pressure thermochemical process, which produces a liquid product, often called bio-oil or bi-crude. During the...... hydrothermal liquefaction process, the macromolecules of the biomass are first hydrolyzed and/or degraded into smaller molecules. Many of the produced molecules are unstable and reactive and can recombine into larger ones. During this process, a substantial part of the oxygen in the biomass is removed by...

  13. Nature and extent of electrogenic microbial communities recovered from Juan de Fuca hydrothermal sulfides

    Science.gov (United States)

    Girguis, P. R.; Nielsen, M. E.

    2011-12-01

    Microbes have evolved a variety of metabolic strategies to survive in anaerobic environments, including extracellular electron transfer (EET). Here we present laboratory and in situ experiments revealing that hydrothermal vent microbes employ and depend upon EET to access spatially remote oxidants via semi-conductive pyrite. To simulate the physical and electrochemical conditions in vent sulfides, we constructed a two-chamber flow-through bioelectrochemical reactor in which a pyrite electrode was enclosed in one chamber and subject to simulated hydrothermal conditions. Electroactive biofilms formed solely on pyrite in electrical continuity with oxygenated water. Phylogenetic and metagenomic analyses revealed a diversity of autotrophic and heterotrophic archaea and bacteria, markedly different in composition from the control (pyrite without electrical continuity). To further characterize this phenomenon, we deployed a bioelectrochemical experiment in situ at the hydrothermal vent sulfide "Roane" (2200 m water depth, at the Mothra hydrothermal field, Juan de Fuca ridge). A graphite anode was inserted into a borehole drilled into the base of a hydrothermal sulfide, and connected through a potentiostat to a carbon-fiber cathode on the outside of the vent structure. The in situ experiment produced sustained current and enriched for a distinct microbial community likely associated with EET. The data presented herein reveal the nature and extent of microbial communities that use conductive minerals such as pyrite, though fully reduced, to facilitate the reduction of spatially remote oxidants while maintaining chemical discontinuity. Thus EET, by enabling sustained access to terminal electron acceptors while maintaining the functioning of strictly anaerobic metabolisms, may alleviate the limitations commonly associated with anaerobic environs, namely the depletion of oxidants.

  14. Poroelastic response of mid-ocean ridge hydrothermal systems to ocean tidal loading: Implications for shallow permeability structure

    Science.gov (United States)

    Barreyre, Thibaut; Sohn, Robert A.

    2016-02-01

    We use the time delay between tidal loading and exit-fluid temperature response for hydrothermal vents to model the poroelastic behavior and shallow upflow zone (SUZ) effective permeability structure of three mid-ocean ridge (MOR) sites with different spreading rates. Hydrothermal vents at Lucky Strike field exhibit relatively small phase lags corresponding to high SUZ effective permeabilities of ≥ ~10-10 m2, with variations that we interpret as resulting from differences in the extrusive layer thickness. By contrast, vents at East Pacific Rise site exhibit relatively large phase lags corresponding to low SUZ effective permeabilities of ≤ ~10-13 m2. Vents at Main Endeavour field exhibit both high and low phase lags, suggestive of a transitional behavior. Our results demonstrate that tidal forcing perturbs hydrothermal flow across the global MOR system, even in places where the tidal amplitude is very low, and that the flow response can be used to constrain variations in SUZ permeability structure beneath individual vent fields.

  15. Spatial patterns of Aquificales in deep-sea vents along the Eastern Lau Spreading Center (SW Pacific).

    Science.gov (United States)

    Ferrera, Isabel; Banta, Amy B; Reysenbach, Anna-Louise

    2014-09-01

    The microbial diversity associated with actively venting deep-sea hydrothermal deposits is tightly connected to the geochemistry of the hydrothermal fluids. Although the dominant members of these deposits drive the structure of the microbial communities, it is less well understood whether the lower abundance groups are as closely connected to the geochemical milieu, or driven perhaps by biotic factors such as microbial community interactions. We used the natural geochemical gradients that exist in the back-arc basin, Eastern Lau Spreading Center and Valu-Fa Ridge (ELSC/VFR) in the Southwestern Pacific, to explore whether the chemolithotrophic Aquificales are influenced by geographical location, host-rock of the vent field or deposit type. Using a combination of cloning, DNA fingerprinting (DGGE) and enrichment culturing approaches, all genera of this order previously described at marine vents were detected, i.e., Desulfurobacterium, Thermovibrio, Aquifex, Hydrogenivirga, Persephonella and Hydrogenothermus. The comparison between clone libraries and DGGE showed similar patterns of distribution of different Aquificales whereas results differed for the enrichment cultures that were retrieved. However, the use of cultivation-based and -independent methods did provide complementary phylogenetic diversity overview of the Aquificales in these systems. Together, this survey revealed that the ELSC/VFR contains some of the largest diversity of Aquificales ever reported at a deep-sea vent area, that the diversity patterns are tied to the geography and geochemistry of the system, and that this geochemical diverse back-arc basin may harbor new members of the Aquificales. PMID:24862554

  16. Variability in the microbial communities and hydrothermal fluid chemistry at the newly discovered Mariner hydrothermal field, southern Lau Basin

    Science.gov (United States)

    Takai, Ken; Nunoura, Takuro; Ishibashi, Jun-Ichiro; Lupton, John; Suzuki, Ryohei; Hamasaki, Hiroshi; Ueno, Yuichiro; Kawagucci, Shinsuke; Gamo, Toshitaka; Suzuki, Yohey; Hirayama, Hisako; Horikoshi, Koki

    2008-06-01

    A newly discovered hydrothermal field called the Mariner field on the Valu Fa Ridge in the southern Lau Basin was explored and characterized with geochemical and microbiological analyses. The hydrothermal fluid discharging from the most vigorous vent (Snow Chimney, maximum discharge temperature 365°C) was boiling at the seafloor at a depth of 1908 m, and two distinct end-member hydrothermal fluids were identified. The fluid chemistry of the typical Cl-enriched and Cl-depleted hydrothermal fluids was analyzed, as was the mineralogy of the host chimney structures. The variability in the fluid chemistry was potentially controlled by the subseafloor phase-separation (vapor loss process) and the microbial community activities. Microbial community structures in three chimney structures were investigated using culture-dependent and -independent techniques. The small subunit (SSU) rRNA gene clone analysis revealed that both bacterial and archaeal rRNA gene communities on the chimney surfaces differed among three chimneys. Cultivation analysis demonstrated significant variation in the culturability of various microbial components among the chimneys, particularly of thermophilic H2-oxidizing (and S-oxidizing) chemolithoautotrophs such as the genera Aquifex and Persephonella. The physical and chemical environments of chimney surface habitats are still unresolved and do not directly extrapolate the environments of possible subseafloor habitats. However, the variability in microbial community found in the chimneys also provides an insight into the different biogeochemical interactions potentially affected by the phase separation of the hydrothermal fluids in the subseafloor hydrothermal habitats. In addition, comparison with other deep-sea hydrothermal systems revealed that the Mariner field microbial communities have unusual characteristics.

  17. Free-living nematode species (Nematoda) dwelling in hydrothermal sites of the North Mid-Atlantic Ridge

    Science.gov (United States)

    Tchesunov, Alexei V.

    2015-12-01

    Morphological descriptions of seven free-living nematode species from hydrothermal sites of the Mid-Atlantic Ridge are presented. Four of them are new for science: Paracanthonchus olgae sp. n. (Chromadorida, Cyatholaimidae), Prochromadora helenae sp. n. (Chromadorida, Chromadoridae), Prochaetosoma ventriverruca sp. n. (Desmodorida, Draconematidae) and Leptolaimus hydrothermalis sp. n. (Plectida, Leptolaimidae). Two species have been previously recorded in hydrothermal habitats, and one species is recorded for the first time in such an environment. Oncholaimus scanicus (Enoplida, Oncholaimidae) was formerly known from only the type locality in non-hydrothermal shallow milieu of the Norway Sea. O. scanicus is a very abundant species in Menez Gwen, Lucky Strike and Lost City hydrothermal sites, and population of the last locality differs from other two in some morphometric characteristics. Desmodora marci (Desmodorida, Desmodoridae) was previously known from other remote deep-sea hydrothermal localities in south-western and north-eastern Pacific. Halomonhystera vandoverae (Monhysterida, Monhysteridae) was described and repeatedly found in mass in Snake Pit hydrothermal site. The whole hydrothermal nematode assemblages are featured by low diversity in comparison with either shelf or deep-sea non-hydrothermal communities. The nematode species list of the Atlantic hydrothermal vents consists of representatives of common shallow-water genera; the new species are also related to some shelf species. On the average, the hydrothermal species differ from those of slope and abyssal plains of comparable depths by larger sizes, diversity of buccal structures, presence of food content in the gut and ripe eggs in uteri.

  18. Investigation into extremely acidic hydrothermal fluids off Kueishan Tao, Taiwan, China

    Institute of Scientific and Technical Information of China (English)

    CHEN Chentung A; WANG Bingjye; HUANG Jungfu; LOU Jiannyuh; KUO Fuwen; TU Yuehyuan; TSAI Hsienshiow

    2005-01-01

    Kueishan Tao (24°51'N, 121 °55′E) is located at a tectonic junction of the fault system extension of Taiwan and the southern rifting end of the Okinawa Trough. A cluster of over 30 vents, at a water depth of about 10~20 m off the eastern tip of the tao emits hydrothermal fluids and volcanic gases such as H2S. A sulfur chimney or mound, formed by condensation of the sulfur contained in the hydrothermal fluid, can usually be seen around the vents. The tallest chimney reaches 6 m. Vents discharging a yellowish fluid have temperatures between 92 and 116 ℃ and flow rates as high as 158 t/h; vents discharging a whitish fluid have lower temperatures of between 48 and 62 ℃ and lower flow rates of about 7.0 t/h. These world-record, breaking low pH (as low as 1.52) fluids are totally different from those found in the black and white-chimneys of the mid-ocean ridges. Magnesium and SiO2 data indicate that these hydrothermal fluids probably originate from a depth of 915~1 350 m below the surface.While the ratios of major ions relative to the sodium of these hydrothermal fluids are quite similar to open ocean water, the ratios of SO4 and chloride to sodium seem to be higher for some of the vents. It is suggested that the volcanic gases contribute SO4 and ohlorine to the fluids, hence increasing their ratios relative to sodium. Some hydrothermal fluids, however, are found to be depleted of the major elements which can have been caused by phase separation. The concentrations of iron and manganese in the fluids are much lower than those found in the mid-ocean ridges, while the aluminium content is higher. Four species of benthos (Xenograpsus testudinatus, a snail, a sea anemone, and a Sipuncala), 1 species of algae (Corallinaceae), and 1 species of fish (Siganus fusescens) were recorded near the hydrothermal vents. A mitoehondria DNA sequence comparison of Xenograpsus testudinatus with 6 other decapod species shows the greatest number of nitrogen base differences in the

  19. Sublacustrine hydrothermal seeps in Northern Lake Tanganyika, East African Rift: 1991 Tanganydro expedition

    Energy Technology Data Exchange (ETDEWEB)

    Tanganydro Group

    1992-06-01

    Sublacustrine hydrothermal seeps were recently discovered (April 1987) at Pemba and Cape Banza along the Zaire side of the North Tanganyika Trough, in the Western Branch of the East African Rift System. This activity as well as two newly discovered site, Luhanga and Mbemvi, have been investigated. This paper presents the main field results on the North Tanganyika hydrothermal sites, and comparisons with data obtained during the 1987 field work. The Pemba and Luhanga sites are located along the seismically active Uvira Border Fault. Two wide bubble fields and eleven active vents of varying morphologies have been observed at Pemba down to a water depth of 46 m, with fluids pouring out at a temperature of 53-88 deg C. These sites are located at the intersection of N120-140 deg Precambrian cleavage and N45-50 deg open fractures. N120 deg-trending veins of massive sulphides (pyrite and marcasite) up to 0.70 m wide associated with these vents have been found locally. Methane and heavier hydrocarbon gases have been identified in the Pemba fluids. Fossil hydrothermal activity was also recognized on land. Fifteen km north of Pemba, the new discovered site of Luhanga has shown 10 active vents