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Sample records for algansea girard cypriniformes

  1. A new species of Algansea (Actinopterygii: Cyprinidae) from the Ameca River basin, in Central Mexico Una especie nueva de Algansea (Actinopterygii: Cyprinidae) en la cuenca del río Ameca en el centro de México

    OpenAIRE

    Rodolfo Pérez-Rodríguez; Gerardo Pérez-Ponce de León; Omar Domínguez-Domínguez; Ignacio Doadrio

    2009-01-01

    A morphological comparative analysis was performed among different populations of the cyprinid Algansea tincella Valenciennes, 1844 from the Lerma-Chapala and Ameca River basins in central Mexico. A new species, Algansea amecae n. sp. is described from individuals collected from small tributary in the headwaters of the Ameca basin. The new species differs from Lerma-Chapala populations of A. tincella by having a lower number of transversal scales, a lower number of infraorbital pores, a promi...

  2. A new species of Algansea (Actinopterygii: Cyprinidae from the Ameca River basin, in Central Mexico Una especie nueva de Algansea (Actinopterygii: Cyprinidae en la cuenca del río Ameca en el centro de México

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    Rodolfo Pérez-Rodríguez

    2009-08-01

    Full Text Available A morphological comparative analysis was performed among different populations of the cyprinid Algansea tincella Valenciennes, 1844 from the Lerma-Chapala and Ameca River basins in central Mexico. A new species, Algansea amecae n. sp. is described from individuals collected from small tributary in the headwaters of the Ameca basin. The new species differs from Lerma-Chapala populations of A. tincella by having a lower number of transversal scales, a lower number of infraorbital pores, a prominent dark lateral stripe along the body, a black caudal spot extending onto the medial caudal inter-radial membranes, and a pigmented ("dotted" lateral line. This new species increases the high level of endemism in the freshwater ichthyofauna of the Ameca basin. It appears to be most closely related to populations in the Lerma-Chapala-Santiago system, as is the case for several other species in the Ameca basin. This pattern of relationship provides evidence for a historical connection between the 2 basins, and implies that a vicariance event led to the isolation of populations and a subsequent speciation event. Due to the limited distributional range of Algansea amecae n. sp., and the environmental deterioration of the Ameca River, we propose that this new species should be designated as a protected species under Mexican law.Se realizó un análisis morfológico comparando diferentes poblaciones del ciprínido Algansea tincella Valenciennes, 1844 correspondientes a los sistemas hidrológicos Lerma-Chapala y cuenca del río Ameca. Con base en este análisis se describe una nueva especie, Algansea amecae n. sp. a partir de los individuos recolectados en un pequeño afluente del alto Ameca, en el centro de México. La nueva especie difiere de las poblaciones de A. tincella del sistema Lerma-Chapala-Santiago por presentar un menor número de escamas transversales, un menor número de poros infraorbitales, una franja obscura lateral muy marcada a lo largo del

  3. The ends of violence. Girard and Derrida

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    Andrew J. McKenna

    2011-12-01

    Full Text Available Jacques Derrida’s critique of philosophical origins, in his essay on Plato and elsewhere, unveils a sacrificial dynamic that René Girard hypothesizes as the origin of human culture. Girard’s latest book, Achever Clausewitz (2008, applies his mimetic theory to history: the Prussian general’s analysis of increasingly violent «reciprocal action» in modern, post-revolutionary warfare exposes the mimetic principle of lethally violent doubles. This «trend to extremes» works to the dissolution of institutions – national sovereignty, international law, politics, war itself – that Derrida explores in Voyous, his book-length essay on terrorism (2003. Both authors see the world of globalized commerce and the globalized terrorism that goes with it as enmeshed in violent undifferentiation. Girard’s historically grounded work supplies a narrative line to Derrida’s structural analyses. Derrida’s call for an ever more vigorous deconstructive rationality as a solution is symptomatic of philosophy’s blindness to the interactive crescendos of human violence that is unveiled in Girard’s religious anthropology.

  4. Evolutionary trends of the pharyngeal dentition in Cypriniformes (Actinopterygii: Ostariophysi.

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    Emmanuel Pasco-Viel

    Full Text Available BACKGROUND: The fish order Cypriniformes is one of the most diverse ray-finned fish groups in the world with more than 3000 recognized species. Cypriniformes are characterized by a striking distribution of their dentition: namely the absence of oral teeth and presence of pharyngeal teeth on the last gill arch (fifth ceratobranchial. Despite this limited localisation, the diversity of tooth patterns in Cypriniformes is astonishing. Here we provide a further description of this diversity using X-ray microtomography and we map the resulting dental characters on a phylogenetic tree to explore evolutionary trends. RESULTS: We performed a pilot survey of dental formulae and individual tooth shapes in 34 adult species of Cypriniformes by X-ray microtomography (using either conventional X-ray machine, or synchrotron microtomography when necessary or by dissecting. By mapping morphological results in a phylogenetic tree, it emerges that the two super-families Cobitoidea and Cyprinoidea have followed two distinct evolutionary pathways. Furthermore, our analysis supports the hypothesis of a three-row dentition as ancestral for Cyprinoidea and a general trend in tooth row reduction in most derived lineages. Yet, this general scheme must be considered with caution as several events of tooth row gain and loss have occurred during evolutionary history of Cyprinoidea. SIGNIFICANCE: Dentition diversity in Cypriniformes constitutes an excellent model to study the evolution of complex morphological structures. This morphological survey clearly advocates for extending the use of X-ray microtomography to study tooth morphology in Cypriniformes. Yet, our survey also underlines that improved knowledge of Cypriniformes life traits, such as feeding habits, is required as current knowledge is not sufficient to conclude on the link between diet and dental morphology.

  5. Schistura udomritthiruji, a new loach from southern Thailand (Cypriniformes: Nemacheilidae)

    Czech Academy of Sciences Publication Activity Database

    Bohlen, Jörg; Šlechtová, Vendula

    2009-01-01

    Roč. 20, č. 4 (2009), s. 319-324. ISSN 0936-9902 R&D Projects: GA ČR GA206/08/0637; GA ČR GA206/05/2556; GA AV ČR IAA600450508; GA MŠk LC06073 Institutional research plan: CEZ:AV0Z50450515 Keywords : Cypriniformes * Nemacheilidae * Schistura udomritthuji Subject RIV: EG - Zoology Impact factor: 0.940, year: 2009

  6. Girards Job-læsning 20 år efter

    DEFF Research Database (Denmark)

    Jensen, Hans Jørgen Lundager

    2007-01-01

      Abstract: Rereading René Girard's book "Job - the Victim of his People" 20 years after its initial appearance in French, the reader encounters not only the interesting phenomenon of an approach to a central Biblical book from "outside" traditional scholarly Bible exegesis which none the less...

  7. Sorcery and publicity: the Cadière-Girard scandal of 1730-1731.

    Science.gov (United States)

    Kuznicki, Jason T

    2007-01-01

    The Cadière-Girard trial of 1730-1731 is an early example of a sensational, nationally publicized French trial in which the major parties were private individuals. Cadière, a female penitent, accused Girard, her Jesuit confessor, of bewitching and raping her; Girard claimed that Cadière was guilty of slander. It was to be the last witchcraft trial in the francophone world. Another notable feature of the trial was its publicity, in which the contesting parties almost immediately became stand-ins for the Society of Jesus and for its Jansenist adversaries. This paper argues that certain anti-Jesuits, particularly Cadière's defence team and in the Parlement of Aix-en-Provence, acted to prolong the trial with the aim of creating as much bad publicity as possible for the Society of Jesus; it also shows how Jansenist publicists took advantage of the lengthy process, creating literature that "burned Girard in spirit," and with him, the Jesuits as a whole. PMID:20737720

  8. Complete mitochondrial DNA genome of Zacco platypus (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Ueng, Yih-Tsong; Chen, Kun-Neng; Han, Chiao-Chuan; Cheng, Chung-Yao; Li, Yi-Min

    2015-04-01

    The complete mitochondrial genome of Zacco platypus (Cypriniformes, Cyprinidae), which has broader distribution range and diverse genetic structure than other species under the genus Zacco, was first determined in this study. The mitochondrial genome is 16,612 base pairs (bp) in length, encoding 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs and 1 non-coding control region. Its gene arrangement and translation direction were identical to those of other typical vertebrate. Control region (D-Loop), of 929 bp lengths long, is located between tRNA(Pro) and tRNA(Phe). The overall base composition of the heavy strand shows T 27.02%, C 26.23%, A 28.94% and G 17.82%, with a slight AT bias of 55.95%. PMID:24047169

  9. The complete mitochondrial genome of Rhynchocypris oxycephalus (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Sui, Xiaoyun; Liang, Yangyang; He, Dekui

    2016-09-01

    The Rhynchocypris oxycephalus (Cypriniformes: Cyprinidae) is an ecological important fish in East Asia. In this study, we firstly sequenced the complete mitochondrial genome of R. oxycephalus. The mitogenome is 16,606 bp in length, including 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes, and two non-coding regions (origin of light-strand replication and control region). The gene content and order is in accord with the common vertebrate form. The nucleotide base composition of H-strand is 28.4% A, 27.2% T, 26.4% C and 18.0% G. The complete mitochondrial genome data would be useful for further studies of genetics, conservation biology and adaptive evolution of R. oxycephalus. PMID:25799350

  10. Reduction of Zygomatic Fractures Using the Carroll-Girard T-bar Screw

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    Ji Eun Baek

    2012-09-01

    Full Text Available Zygomatic fractures are the second most common facial bone fractures encountered andtreated by plastic surgeons. Stable fixation of fractured fragments after adequate exposureis critical for ensuring three-dimensional anatomic reduction. Between January 2008 andDecember 2010, 17 patients with zygomatic fractures were admitted to our hospital; therewere 15 male and 2 female patients. The average age of the patients was 41 years (range, 19to 75 years. We exposed the inferior orbital rim and zygomatic complex through a lateralbrow, intraoral, and subciliary incisions, which allowed for visualization of the bone, andthen the fractured parts were corrected using the Carroll-Girard T-bar screw. Postoperativecomplications such as malar asymmetry, diplopia, enophthalmos, and postoperative infectionwere not observed. Lower eyelid retraction and temporary ectropion occurred in 1 of the 17patients. Functional and cosmetic results were excellent in nearly all of the cases. In this report,we describe using the Carroll-Girard T-bar screw for the reduction of zygomatic fractures.Because this instrument is easy to use and can rotate to any direction and vector, it can beused to correct displaced zygomatic bone more accurately and safely than other devices,without leaving facial scars.

  11. The complete mitochondrial genome of Chinese sucker Myxocyprinus asiaticus (Cypriniformes, Catostomidae).

    Science.gov (United States)

    Chen, I-Shiung

    2013-12-01

    In this study, the complete mitogenome sequence of Chinese sucker Myxocyprinus asiaticus (Cypriniformes, Catostomidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,636 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes, and a noncoding control region (CR). CR of 944 bp length is located between tRNA(Pro) and tRNA(Phe). The overall base composition of M. asiaticus is 28.62% for A, 27.90% for C, 24.8% for T, and 18.70% for G, with a slight AT bias of 53.40%. The complete mitogenome may provide important DNA molecular data for further phylogenetic analyses for higher taxa of teleost fishes especially for the members of Cypriniformes. PMID:23544832

  12. Mimesi i reciprocitat. René Girard i la nostàlgia comunitària de transcendència

    OpenAIRE

    Garcia-Duran Bayona, Francisco Javier

    2015-01-01

    El nucli d’estudi d’aquesta tesi és el pensament de René Girard, que es pot situar al llarg de la segona meitat del segle XX: des de Mensonge romantique et vérité romanesque (1961) a Achever Clausewitz (2007). Aquest pensament es va definint, al llarg de la tesi, en dues direccions: el pensament sobre Girard i el pensament a partir de Girard. La primera direcció ordena el pensament d’aquest autor en tres cercles, segons un criteri cronològic i ––alhora–– un de temàtic. El primer cercle és...

  13. Complete mitochondrial genome of the Korean endemic species Microphysogobio yaluensis (Teleostei, Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Park, Chang Eon; Park, Gun-Seok; Kim, Min-Chul; Kim, Kgu-Hwan; Park, Hee Cheon; Lee, In-Jung; Shin, Jae-Ho

    2016-09-01

    In this study, we sequenced the complete mitochondrial genome of the Korean endemic species Microphysogobio yaluensis (Teleostei, Cypriniformes, Cyprinidae). The mitogenome, consisted of 16 601 base pairs (bp), encoding 13 protein-coding genes (PCGs), 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and 2 non-coding regions. The overall base composition of M. yaluensis was G + C: 43.8%, A + T: 56.2%, apparently with a slight AT bias. Phylogenetic analysis showed that M. yaluensis was close to Hemibarbus mylodon. PMID:26260172

  14. The complete mitochondrial genome sequence of Squalidus japonicus coreanus (Teleostei, Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Eon Park, Chang; Park, Gun-Seok; Kwak, Yunyoung; Kim, Min-Chul; Kim, Kgu-Hwan; Park, Hee Cheon; Shin, Jae-Ho

    2016-09-01

    The short-barbel gudgeon Squalidus japonicus coreanus (Teleostei, Cypriniformes, Cyprinidae) is a subspecies of S. japonicus, and is endemic to Korea. The complete mitochondrial genome sequence consisted of 16 597 base pairs (bp) encoding 13 protein-coding genes (PCGs), two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and two non-coding regions. The overall base composition of S. japonicus coreanus was G + C: 47.4% and A + T: 52.6%, apparently with a slight AT bias. Phylogenetic analysis showed that S. japonicus coreanus was closely related to Pungtungia herzi. PMID:26329666

  15. The Effect of Limonium sinense (Girard Kuntze on Fatigue and Recovery after Exercise

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    Yanxia Cao

    2015-04-01

    Full Text Available To evaluate the physical and chemical properties of polysaccharides from selenium-rich tea and the effects of these polysaccharides on swimming performance and recovery from swimming-induced fatigue in mice. By setting up a 5-week endurance exercise model in mice and the mice were given by oral gavage different doses of Limonium sinense (Girard Kuntze (LSK. The swimming exhaustion time of mice was recorded and the concentrations of blood glucose, Blood Ureanitrogen (BUN, Blood Lactic Acid (BLA and the contents of hepatic glycogen and Malonaldehyde (MDA as well as the activity of Hepatic Glutathione Peroxidase (GSH-Px were measured immediately and at 24 h after exhaustive exercise. LSK were Se-binding glycoproteins and could prolong the swimming exhaustion time, improve the carbohydrate metabolism, significantly increase the activity of GSH-Px and reduce the content of MDA in liver tissue of mice. LSK have an anti-fatigue effect in mice and the underlying mechanism is related to regulating the carbohydrate metabolism and improving tissue lipid peroxidation caused by excessive exercise.

  16. Análise das atividades biológicas dos venenos de Philodryas olfersii (Lichtenstein) e P. patagoniensis (Girard) (Serpentes, Colubridae) Analysis of biological activities from Philodryas olfersii (Lichtenstein) and P. patagoniensis (Girard) venoms (Serpents, Colubridae)

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    Marisa M. T. da Rocha; Maria de F. D. Furtado

    2007-01-01

    Philodryas olfersii (Lichtenstein, 1823) e P.patagonienis (Girard, 1857) são serpentes colubrídeas da série opistóglifa, restritas à América do Sul. Vários acidentes ocasionados por estas serpentes têm sido relatados, caracterizando-se por ação local importante: dor, edema e hemorragia. É um acidente muito semelhante àquele causado por serpentes do gênero Bothrops Wagler, 1824 e muitas vezes os pacientes são tratados com soro antibotrópico. Poucos estudos tratam da caracterização destes venen...

  17. The complete mitochondrial genome of the Zacco platypus, Huangshan, China (Cypriniformes: Cyprinidae, subfamily Daninninae).

    Science.gov (United States)

    Cheng, Shuang-Huai; Yan, Jin-Jin; Yan, Yun-Zhi; Zhang, Yi; Xia, Meng-Ning; Liu, Ying-Long; Lu, Yan-Mei

    2015-01-01

    The mitochondrial genome of Zacco platypus (Cypriniformes: Cyprinidae, subfamily Daninninae) is a circular molecule of 16,611 bp in length, containing 37 typical animal mitochondrial genes: 13 protein-coding genes (PCGs), 2 ribosomal RNAs, 22 transfer RNAs and a D-loop region. Its gene order and arrangement are identical to the common type found in most fish mitogenomes. All PCGs start with a typical ATG codon except for COI which use GTG as start codon; all PCGs terminate in the common stop codon TAA or TAG, except for the COII which use single T as stop codon. The D-loop region is 928 bp long, located between tRNAPro and tRNAPhe genes. It contains some structures of repeated motifs and microsatellite-like elements characteristic of the Cyprinidae. PMID:24409889

  18. Complete mitochondrial genome of the Pseudogastromyzon jiulongjiangensis Chen (Cypriniformes, Homalopteridae) and phylogenetic analysis of the Cyprinoidei.

    Science.gov (United States)

    Lv, Yaoping; Zhang, Kaibo; Liu, Ziming; Hu, Zehui; Wang, Kaiwei; Zhou, Haidong

    2016-07-01

    The Pseudogastromyzon jiulongjiangensis Chen (Cypriniformes, Homalopteridae) is a promising ornamental and commercial candidate in China. In this study, the complete mitochondrial genome of P. jiulongjiangensis was first determined. It is 16,571 bp length and consists of 22 tRNA genes, 13 protein-coding genes, two rRNA genes, and a control region. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. Phylogenetic analysis revealed that P. jiulongjiangensis, Formosania lacustris, and other seven fish first clustered into the Homalopteridae clade. Then, the Homalopteridae and Cobitidae formed the sister group. The Catostomoidae and Cyprinidae constituted the sister branch, which is inconsistent with the previous phenotypic report. It is suggested that the taxonomic research might lose some significant evolutionary characters. This study will contribute to phylogenetic analysis of the Homalopteridae and the natural resources conservation of P. jiulongjiangensis. PMID:27158787

  19. The complete mitochondrial genome sequence of Diptychus maculatus Steindachner (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    He, Dekui; Liang, Yangyang; Li, Chunhua; Wei, Chaojun; Chen, Yifeng

    2016-09-01

    Diptychus maculatus (Cypriniformes: Cyprinidae), the sole species of genus Diptychus, is an economically important freshwater fish widely distributed in Asia. In this study, we first sequenced the complete mitochondrial genome of D. maculatus. The genome is 16,835 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes as well as 2 non-coding regions: origin of light-strand replication (OL) and control region (D-loop). The gene composition and order are identical with most bony fishes. The overall nucleotide composition of the heavy strand is 27.7% A, 26.7% T, 26.5% C and 19.1% G. The mitogenome data would be useful for further genetics studies, phylogenetic analysis, and resource protection of D. maculatus and phylogenetic analysis of Schizothoracine fishes. PMID:25774947

  20. Gaïd Girard ed. Le Superflu, chose très nécessaire.

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    Nathalie Vanfasse

    2006-04-01

    Full Text Available Ces Actes d’un colloque organisé par le Centre d’Études Interdisciplinaires du Monde Anglophone à l’Université de Brest fin novembre 2002, rassemblés et présentés par Gaïd Girard offrent un volume riche et agréable à lire. L’ouvrage part du couple superflu/nécessaire rendu célèbre par Voltaire et, en cinq parties, s’emploie avec succès à dépasser l’opposition entre ces deux termes.La première partie, « Poétique et politique du superflu », s’intéresse aux rapports entre superflu et écriture. J...

  1. Análise das atividades biológicas dos venenos de Philodryas olfersii (Lichtenstein e P. patagoniensis (Girard (Serpentes, Colubridae Analysis of biological activities from Philodryas olfersii (Lichtenstein and P. patagoniensis (Girard venoms (Serpents, Colubridae

    Directory of Open Access Journals (Sweden)

    Marisa M. T. da Rocha

    2007-06-01

    Full Text Available Philodryas olfersii (Lichtenstein, 1823 e P.patagonienis (Girard, 1857 são serpentes colubrídeas da série opistóglifa, restritas à América do Sul. Vários acidentes ocasionados por estas serpentes têm sido relatados, caracterizando-se por ação local importante: dor, edema e hemorragia. É um acidente muito semelhante àquele causado por serpentes do gênero Bothrops Wagler, 1824 e muitas vezes os pacientes são tratados com soro antibotrópico. Poucos estudos tratam da caracterização destes venenos, assim tivemos como objetivo de trabalho o estudo dos venenos de P.olfersii e P.patagonienis. Os venenos apresentaram teor de proteínas entre 75 e 90%. A atividade desfibrinante não foi detectada quando testada em camundongos. O quadro de dor causado pelo envenenamento experimental, em camundongos, mostrou que os venenos de P.olfersii e P.patagoniensis causaram intensa reatividade, sendo que o veneno de P.patagoniensis foi o mais ativo. Ambos os venenos apresentaram dose mínima edematogênica em torno de 1 µg/camundongo com ação máxima em 30 minutos.A ação hemorrágica se instalou rapidamente, com doses mínimas semelhantes. As atividades tóxicas foram semelhantes, com valores em torno de 60,0 µg/camundongo, comparáveis aos venenos botrópicos.Philodryas olfersii (Lichtenstein, 1823 and P. patagoniesis (Girard, 1857 are species of colubrid snakes of the opisthoglyphous series restricted to South America. Several accidents caused by these snakes have been reported and they are mainly characterized by marked effects at the site of bite, such as pain, swelling and hemorrhage. Such accidents are similar to those caused by snakes of the genus Bothrops, and thereby patients bitten by Philodryas sp. are frequently treated with Bothrops antivenom. Since few studies have dealt with the characterization of these venoms, our aim was to study the venoms of P.olfersii and P.patagoniensis. They presented protein contents between 75 and 90

  2. Phylogeny of the Labeoninae (Teleostei, Cypriniformes) based on nuclear DNA sequences and implications on character evolution and biogeography

    Institute of Scientific and Technical Information of China (English)

    Lanping ZHENG; Junxing YANG; Xiaoyong CHEN

    2012-01-01

    The Labeoninae is a subfamily of the family Cyprinidae,Order Cypriniformes.Oromandibular morphology within the Labeoninae is the greatest among cyprinid fishes.Although several phylogenetic studies about labeonines have been undertaken the results have been inconsistent and a comprehensive phylogeny is needed.Further,an incongruence between morphological and molecular phylogeny requires a systematic exploration of the significance of morphological characters on the basis of the molecular phylogeny.In this study,a total of 292 nucleotide sequences from 73 individuals (representing 24 genera and 73 species) of Labeoninae were analyzed.The results of the phylogenetic analysis indicate that there are four major clades within Labeoninae and three monophyletic lineages within the fourth clade.Results of the character evolution show that all oromandibular morphological characters are homoplastically distributed on the molecular phylogenetic tree and suggests that these characters evolved several times during the history of labeonines.In particular,the labeonine,a specific disc on the lower lip,has been acquired three times and reversed twice.These morphological characters do not have systematic significance but can be useful for taxonomy.The results of biogeography suggest that the Labeoninae originated from Southeast Asia and separately dispersed to Africa,East Asia and South Asia.

  3. The complete mtDNA genome of Triplophysa dorsalis (Cypriniformes, Balitoridae, Cobitoidea): genome characterization and phylogenetic analysis.

    Science.gov (United States)

    Lei, Dujuan; Conteh Kanu, Unisa; Zhao, Guang; Xie, Peng; Yuan, Hao; Li, Yu; Niu, Jiangong; Ma, Xufa

    2016-09-01

    Based upon the morphological characters, the genus Triplophysa (plateau Loach) is a highly diverse group in the family Balitoridae (or Cypriniformes, Cobitoidea) with 133 valid species. Therefore, the taxonomic relationship of this species at the genetic level remains ambiguous. In the present study, we sequenced the complete mitochondrial genome of Triplophysa dorsalis. In order to understand its position and genetic background at the gene level, the characteristics of mitochondrial DNA sequences and phylogenetic relationship were examined. The mitochondrial genome of T. dorsalis is similar to those of the typical vertebrates, 16 572 bp in length, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a non-coding control region (D-loop). All genes were encoded on the heavy strand except for ND6 and 8 tRNA genes. The overall base composition of the heavy strand was 28.16%, 28.41%, 25.62% and 17.82% for A, T, C and G, respectively. Phylogenetic analyses showed that the seven Triplophysa species clustered together with T. dorsalis to form a sister group with Triplophysa strauchii, T. bleekeri, T. stoliczkai and T. bombifrons. The two genera Triplophysa and Barbatula formed a sister-group relationship, the species Homatula potanini located in the intermediate position, and the genus Leptobotia elongata was in the basal position in the subfamily Nemacheilidae. Further investigations with more Triplophysa species need to be performed for better understanding of the evolutionary history of this fascinating genus. PMID:26457606

  4. cDNA cloning and expression analysis of two distinct Sox8 genes in Paramisgurnus dabryanus (Cypriniformes)

    Indian Academy of Sciences (India)

    Xiaohua Xia; Jie Zhao; Qiyan Du; Zhongjie Chang

    2010-08-01

    The Sox9 gene attracts a lot of attention because of its connection with gonadal development and differentiation. However, Sox8, belonging to the same subgroup SoxE, has rarely been studied. To investigate the function as well as the evolutionary origin of SOXE subgroup, we amplified the genomic DNA of Paramisgurnus dabryanu using a pair of degenerate primers. Using rapid amplification of the cDNA ends (RACE), it was discovered that P. dabryanu has two duplicates: Sox8a and Sox8b. Each has an intron of different length in the conserved HMG-box region. The overall sequence similarity of the deduced amino acid of PdSox8a and PdSox8b was 46.26%, and only two amino acids changed in the HMG-box. This is the first evidence showing that there are two distinct duplications of Sox8 genes in Cypriniformes. Southern blot analysis showed only one hybrid band, with lengths 7.4 or 9.2 kb. Both semi-quantitative RT-PCR and real-time quantitative PCR assay displayed that both PdSox8a and PdSox8b are downregulated during early embryonic development. In adult tissues, the two Sox8 genes expressed ubiquitously, and expression levels are particularly high in the gonads and brain. In gonads, both PdSox8a and PdSox8b are expressed at a higher level in the tesis than in the ovary. PdSox8a and PdSox8b may have functional overlaps and are essential for the neuronal development and differentiation of gonads.

  5. cDNA cloning and expression analysis of two distinct Sox8 genes in Paramisgurnus dabryanus (Cypriniformes).

    Science.gov (United States)

    Xia, Xiaohua; Zhao, Jie; Du, Qiyan; Chang, Zhongjie

    2010-08-01

    The Sox9 gene attracts a lot of attention because of its connection with gonadal development and differentiation. However, Sox8, belonging to the same subgroup SoxE, has rarely been studied. To investigate the function as well as the evolutionary origin of SOXE subgroup, we amplified the genomic DNA of Paramisgurnus dabryanu using a pair of degenerate primers. Using rapid amplification of the cDNA ends (RACE), it was discovered that P. dabryanu has two duplicates: Sox8a and Sox8b. Each has an intron of different length in the conserved HMG-box region. The overall sequence similarity of the deduced amino acid of PdSox8a and PdSox8b was 46.26%, and only two amino acids changed in the HMG-box. This is the first evidence showing that there are two distinct duplications of Sox8 genes in Cypriniformes. Southern blot analysis showed only one hybrid band, with lengths 7.4 or 9.2 kb. Both semi-quantitative RT-PCR and real-time quantitative PCR assay displayed that both PdSox8a and PdSox8b are downregulated during early embryonic development. In adult tissues, the two Sox8 genes expressed ubiquitously, and expression levels are particularly high in the gonads and brain. In gonads, both PdSox8a and PdSox8b are expressed at a higher level in the tesis than in the ovary. PdSox8a and PdSox8b may have functional overlaps and are essential for the neuronal development and differentiation of gonads. PMID:20861569

  6. Length-weight and length-length relationships of seven loach species (Teleostei: Cypriniformes from five localities in northern Western Ghats, India

    Directory of Open Access Journals (Sweden)

    Ashwini Keskar

    2015-12-01

    Full Text Available Length-weight (LWR and length-length (LLR relationships of seven loach species (Teleostei: Cypriniformes: Botia striata, Lepidocephalichthys thermalis, Paracanthocobitis mooreh, Indoreonectes evezardi, Nemacheilus anguilla, Nemachilichthys rueppelli and Schistura denisoni were studied from five localities within the Krishna River system of the Indian Western Ghats: Lonawala (Indrayani River, Paud (Mula River, Warje (Mutha River, Bhor (Nira River and Patan (Koyna River.  With the exception of L. thermalis all species are endemic to peninsular India, and to our knowledge this is the first presentation of LWR and LLR data for them.  New maximum lengths are also reported for I. evezardi, N. anguilla, N. rueppelli and S. denisoni. 

  7. Using the whole body as a sucker: combining respiration and feeding with an attached lifestyle in hill stream loaches (Balitoridae, Cypriniformes).

    Science.gov (United States)

    De Meyer, Jens; Geerinckx, Tom

    2014-09-01

    Small fishes living in fast-flowing rivers face a harsh environment as they can easily be swept away by the rapid currents. To survive such circumstances, teleosts evolved a wide variety of attachment mechanisms, based on friction, negative pressure or both. Balitorinae (Balitoridae, Cypriniformes) are exceptional in using their whole body as an adhesive apparatus. We investigated the morphological adaptations of Balitorinae by studying the osteology and myology of four species (Beaufortia leveretti, Sewellia lineolata, Pseudogastromyzon myersi, and Gastromyzon punctulatus) using clearing and staining, serial cross-sections and CT-scanning. A kinematic analysis was performed to study the respiration and feeding mechanisms and to identify key structures in these mechanisms. Our research showed that the whole body of Balitorinae acts as a suction disc, with friction-enhancing structures (unculi) on the thickened anterior rays of the paired fins. The abruptly rising head profile, supported by the extremely enlarged lacrimal bone and the flat ventral body surface facilitate effective substrate attachment. During attachment, the pelvic girdle is pulled anterodorsally, suggesting the formation of a negative pressure underneath the body. Detachment by water inflow underneath the body is prevented by three mechanisms. 1) Barbels control the water inflow by detachment and reattachment to the substrate. 2) Most water present underneath the body is removed during inspiration. 3) Excess water is regularly removed by movements of the posterior pectoral fin rays. The balitorine body is thus modified as such that it allows effective attachment, while not impairing respiration. Comparison with other teleosts living in similar environments shows that most species use more locally concentrated modifications of the paired fins and/or the mouth for attachment. The high diversity in teleostean adhesive apparatuses and associated myological modifications suggest a substantial functional

  8. Predation and control efficacies of Misgurnus mizolepis (Cypriniformes: Cobitidae) toward Culex pipiens molestus (Diptera: Culicidae) and fish toxicity of temephos in laboratory and septic tank conditions.

    Science.gov (United States)

    Chae, Seong Chun; Kwon, Young Hyun; Min, Kyung Il; Kim, Hyung Soo; Kim, Nam-Jin; Kim, Jun-Ran; Son, Bong Gi; Ahn, Young-Joon

    2014-07-01

    Culex pipiens molestus Forskal (Diptera: Culicidae) is the dominant mosquito species in septic tanks in South Korea. An assessment was made of the biological control potential of mud loaches, Misgurnus mizolepis Günther (Cypriniformes: Cobitidae), toward Cx. p. molestus larvae in laboratory and septic tanks. Results were compared with those of temephos 20% emulsifiable concentrate. In laboratory tests, all mud loaches survived on sedimentation chamber- and effluent chamber-collected water of aerobic septic tanks (ASTs), whereas all mud loaches died within 3-12 h after introduction into sedimentation chamber- and effluent chamber-collected water of anaerobic septic tanks, Gill hyperplasia and hemorrhages at the bases of pectoral fins were detected in all dead mud loaches. These appeared to have been caused by bacterial disease, rather than the physical and chemical characteristics of the septic tank water. A mud loach consumed an average range of 1,072-1,058 larvae of Cx. p. molestus in the AST water at 24 h. At the manufacturer's recommended rate (10 ml/ton) in the AST water, the temephos formulation did not cause fish mortality. In the AST experiment, predation of mosquito larvae by mud loaches at a release rate of one fish per 900 mosquito larvae resulted in complete mosquito control from the third day after treatment throughout the 18-wk survey period, compared with temephos 20% emulsifiable concentrate-treated AST water (reduction rate, 40% at 28 days after treatment). Reasonable mosquito control in aerobic septic tanks can be achieved by mosquito breeding season stocking of a rate of one mud loach per 900 mosquito larvae. PMID:25118414

  9. Acanthocercodes n. g. (Monogenoidea: Diplectanidae) for species parasitising threadfins (Perciformes: Polynemidae), with description of Acanthocercodes bullardi n. sp. from the Atlantic threadfin Polydactylus octonemus (Girard) and reassignment of three species of Diplectanum Monticelli, 1903 from the Indo-Pacific Ocean.

    Science.gov (United States)

    Kritsky, Delane C; Diggles, Ben K

    2015-07-01

    Acanthocercodes n. g. (Diplectanidae) is proposed for Acanthocercodes bullardi n. sp. and three previously described species of Diplectanum all parasites of the gill lamellae of threadfins (Perciformes: Polynemidae). The new genus is characterised by species having peduncular spines composed of an anteriorly directed point and a flattened base from which an anterior root arises. Members of the genus lack auxiliary spinous or sucker-like structures in the haptor. Acanthocercodes bullardi n. sp. is described from the Atlantic threadfin, Polydactylus octonemus (Girard), in the Gulf of Mexico off Louisiana, USA. Diplectanum polynemus Tripathi, 1957 is redescribed and transferred to Acanthocercodes as A. polynemus (Tripathi, 1957) n. comb. based on specimens collected from the fourfinger threadfin, Eleutheronema tetradactylum (Shaw), from the mouth of the River Adelaide, Northern Territory, Australia. Diplectanum spinosum (Maillard & Vala, 1980) (=Pseudodiplectanum spinosum Maillard & Vala, 1980) and Diplectanum megacirrus (Maillard & Vala, 1980) (=Pseudodiplectanum megacirrus Maillard & Vala, 1980) from the lesser African threadfin, Galeoides decadactylus (Bloch), are transferred to Acanthocercodes as A. spinosum (Maillard & Vala, 1980) n. comb. and A. megacirrus (Maillard & Vala, 1980) n. comb., respectively. PMID:26063297

  10. Myxobolus ictiobus n. sp. and Myxobolus minutus n. sp. (Cnidaria: Myxobolidae) from the gills of the smallmouth buffalo Ictiobus bubalus Rafinesque (Cypriniformes: Catostomidae).

    Science.gov (United States)

    Rosser, Thomas G; Griffin, Matt J; Quiniou, Sylvie M A; Alberson, Neely R; Woodyard, Ethan T; Mischke, Charles C; Greenway, Terrence E; Wise, David J; Pote, Linda M

    2016-07-01

    The smallmouth buffalo Ictiobus bubalus Rafinesque (Catostomidae) is native to North American waterways and occasionally grown in pond aquaculture. Species of Myxobolus Bütschli, 1882 have been reported from the gills, integument, and intestinal tract of buffalo fish, although there is ambiguity in some host records. In the summer of 2013, thirteen adult smallmouth buffalo were seined from a 0.1-acre (0.04-hectare) experimental research pond at the Thad Cochran National Warmwater Aquaculture Center in Stoneville, Mississippi, USA, and examined for the presence of parasitic infection. Two previously unknown species of Myxobolus were observed parasitising the gills. Plasmodia of the two species differed from each other in both size and shape. Morphologically the two species were distinct from one another and from other Myxobolus spp. previously reported from buffalo fish. Myxospores of Myxobolus ictiobus n. sp. were spherical and measured 12.7-14.5 (13.9 ± 0.4) µm in length and 10.7-13.6 (12.5 ± 0.7) µm in width with a thickness of 10.3-14.8 (12.6 ± 2.3) µm. Polar capsules measured 5.6-7.4 (6.6 ± 0.4) µm in length and 3.7-4.9 (4.5 ± 0.8) µm in width and each contained a coiled polar filament with 5-6 turns. Myxospores of Myxobolus minutus n. sp. were circular in shape and measured 7.4-9.6 (8.6 ± 0.7) µm in length and 7.5-9.9 (8.8 ± 0.7) µm in width with a thickness of 6.5-7.3 (6.7 ± 0.3) µm. Polar capsules measured 3.6-4.9 (4.3 ± 0.3) µm in length and 2.8-3.8 (3.3 ± 0.3) µm and each contained a coiled polar filament with 5-6 turns. Supplemental 18S rRNA gene sequencing identified unique sequences for each isolate. Phylogenetic analysis of 18S rRNA sequences demonstrated a strong clustering of both isolates with other species of Myxobolus from cypriniform fish. PMID:27307169

  11. René Girard's apocalyptic modernity

    OpenAIRE

    Doran, Robert

    2011-01-01

    This essay examines how René Girard’s most recent book, Achever Clausewitz (2007), expands on Girard’s theory of the sacred to include a more specific and concrete notion of the apocalypse as a historically conditioned concept. In particular, I argue that the idea of “total war” (the kind of war engendered by mass conscription) is crucial to Girard’s critique of Clausewitz, which consists in pointing out the irreconcilability between Clausewitz’s famous formula “war is politics by other means...

  12. 基于Rag1基因序列变异的鲤形目鱼类系统发育重建:采样策略对生命之树的影响%Inferring the Tree of Life of the order Cypriniformes, the earth's most diverse clade of freshwater fishes: Implications of varied taxon and character sampling

    Institute of Scientific and Technical Information of China (English)

    Richard L. MAYDEN; Phillip M. HARRIS; Junbing LI; Xuzhen WANG; Kenji SAITOH; Shunping HE; Huanzhang LIU; Yiyu CHEN; Mutsumi NISHIDA; Masaki MIYA; Kevin L. TANG; Robert M. WOOD; Wei-Jen CHEN; Mary K. AGNEW; Kevin W. CONWAY; Lei YANG; Andrew M. SIMONS; Henry L. BART

    2008-01-01

    The phylogenetic relationships of species are fundamental to any biological investigation, including all evolutionary studies. Accurate inferences of sister group relationships provide the researcher with an historical framework within which the attributes or geographic origin of species (or supraspecific groups) evolved. Taken out of this phylogenetic context, interpretations of evolutionary processes or origins, geographic distributions, or speciation rates and mechanisms, are subject to nothing less than a biological experiment without controls. Cypriniformes is the most diverse clade of freshwater fishes with estimates of diversity of nearly 3,500 species. These fishes display an amazing array of morphological, ecological, behavioral, and geographic diversity and offer a tremendous opportunity to enhance our understanding of the biotic and abiotic factors associated with diversification and adaptation to environments. Given the nearly global distribution of these fishes, they serve as an important model group for a plethora of biological investigations, including indicator species for future climatic changes. The occurrence of the zebrafish, Danio rerio, in this order makes this clade a critical component in understanding and predicting the relationship between mutagenesis and phenotypic expressions in vertebrates, including humans. With the tremendous diversity in Cypriniformes, our understanding of their phylogenetic relationships has not proceeded at an acceptable rate, despite a plethora of morphological and more recent molecular studies. Most studies are pre-Hennigian in origin or include relatively small numbers of taxa. Given that analyses of small numbers of taxa for molecular characters can be compromised by peculiarities of long-branch attraction and nodal-density effect, it is critical that significant progress in our understanding of the relationships of these important fishes occurs with increasing sampling of species to mitigate these potential

  13. BIOLOGY OF PROCAMBARUS CLARKII (GIRARD, 1852 IN LAKE TRASIMENO

    Directory of Open Access Journals (Sweden)

    DÖRR A. J.M.

    2006-01-01

    Full Text Available The aim of this study was to gather more informations about the biological characteristics of Procambarus clarkii and to investigate the reasons of its rapid expansion in Lake Trasimeno. Specimens of both sexes (544 males and 624 females were collected from October 2000 to November 2001. During the sampling period physical factors such as water temperature and hydrologic level of LakeTrasimeno were recorded. The study was conducted monthly in order to assess this species’ reproductive period. 1,168 specimens were analyzed for colour, sex, weight, and length. The sexual maturation of gonads was investigated in females. The results of this study evidenced that the population is in expansion, showing that Lake Trasimeno is an optimal habitat for this species.

  14. Anoressia e mimesi secondo René Girard

    Directory of Open Access Journals (Sweden)

    Rosa Sidella

    2011-01-01

    Full Text Available Anorexia and Mimesis in René Girard’s Thought - One of the basic premises of Girard’s thought is the theory of mimetic desire, for which, in all human relationships, the Other as a model is perceived as a rival. Mimesis is also found in narcissism, in love relationships and eating disorders, such as anorexia and bulimia, which cannot and should not be classified only in psychoanalytic terms. In fact, we should in such cases consider cultural and social contexts, which also impose prescriptions about the care and appearance of the body.

  15. Anoressia e mimesi secondo René Girard

    OpenAIRE

    Rosa Sidella

    2011-01-01

    Anorexia and Mimesis in René Girard’s Thought - One of the basic premises of Girard’s thought is the theory of mimetic desire, for which, in all human relationships, the Other as a model is perceived as a rival. Mimesis is also found in narcissism, in love relationships and eating disorders, such as anorexia and bulimia, which cannot and should not be classified only in psychoanalytic terms. In fact, we should in such cases consider cultural and social contexts, which also impose prescription...

  16. “CULTIVO EXPERIMENTAL DE LA ACÚMARA DEL LAGO DE PÁTZCUARO (Algansea lacustris STEINDACHNER, 1895. PISCES: CYPRINIDAE) EN ESTANQUES RÚSTICOS”

    OpenAIRE

    FABIÁN REGALADO, JOSÉ MANUEL

    2012-01-01

    La acuacultura se define como una actividad productiva orientada hacia el cultivo de los organismos acuáticos incluyendo peces, moluscos, crustáceos y plantas acuáticas. La actividad de cultivo implica necesariamente alguna forma de intervención del ser humano en el proceso de crianza para incrementar la producción, así como el manejo de un lote de organismos de talla regular, su alimentación, la protección de depredadores o el incremento en la sobrevivencia. También implica la...

  17. Complete mitochondrial genome of Rhodeus ocellatus (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Hu, Juan; Chen, Yibin; Zhao, Huihong; Yang, Huirong; Liu, Li

    2016-09-01

    The complete mitogenome sequence of Rhodeus ocellatus (Kner) was determined using the next-generation sequencing (NGS). The genome was 16 761 bp in length and contained 13 protein-coding genes, two ribosomal RNA genes, 22 transfer RNA genes and a control region. The overall nucleotide composition was 30.43% A, 27.50% T, 25.94% C, and 16.13% G, with an A + T bias of 57.93%. The gene composition and the arrangement of the R. ocellatus mitochondrial genome were similar to that of most other vertebrates. The complete mitochondrial genome sequence will help to study the evolutionary relationships and population genetics of Rhodeus fish. PMID:26258516

  18. The complete mitochondrial genome of the Ancherythroculter lini (Cypriniformes, Cyprinidea).

    Science.gov (United States)

    Chen, Yibin; Hu, Juan; Zhao, Huihong; Yang, Huirong; Liu, Li

    2016-07-01

    Ancherythroculter lini is the endemic species of fish in China. In this study, we determined the complete mitochondrial genome of Ancherythroculter lini using the next-generation sequencing (NGS). The total length of the complete mitochondrial genome is 16 616 bp which consists of 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNAs genes, and a control region. Most of the genes are encoded on the heavy strand except ND6 and eight tRNA genes. The genes order and locations are similar to other vertebrates. The H-strand has a nucleotide composition of 30.78% A, 24.74% T, 16.48% G, and 28.00% C. 13 intergenic spacers and five gene overlaps exist in the complete mitochondrial genome. And, we found the culter genus and Ancherythroculter nigrocauda are more closely related to Ancherythroculter lini by constructing a phylogenetic tree. PMID:27158794

  19. The complete mitochondrial genome of Pimephales promelas (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Liu, Sheng; Zhang, Kai; Xiao, Liang

    2016-09-01

    The fathead minnow, Pimephales promelas, is an ecological important fish distributed across North America. In this study, we firstly assembled the complete mitochondrial genome of P. promelas. The mitogenome is 16 709 bp in length, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 2 non-coding regions. The gene content and order is in accord with the common vertebrate form. ATG acts as the start codon for all PCGs except cytochrome c oxidase subunit I (COXI), which has GTG as a start codon. The nucleotide base composition of H-strand is 29.2% A, 27.9% T, 25.9% C, and 17.0% G. The complete mitochondrial genome data would be useful for further studies of reproduction, development, and adaptive evolution of P. promelas. PMID:26328777

  20. Molecular Systematics of the Phoxinin Genus Pteronotropis (Otophysi: Cypriniformes

    Directory of Open Access Journals (Sweden)

    Richard L. Mayden

    2015-01-01

    inclusion of the currently recognized Notropis harperi, herein referred to as Pteronotropis. Two major clades are supported: one inclusive of P. hubbsi, P. welaka, and P. harperi and the second inclusive of P. signipinnis, P. grandipinnis, P. hypselopterus plus P. merlini sister to P. euryzonus, and P. metallicus plus P. stonei.

  1. The mitochondrial genome of Pseudolaubuca sinensis (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Chen, Huijuan; Wang, Dengqiang; Duan, Xinbin; Liu, Shaoping; Chen, Daqing

    2016-09-01

    In the present study, the complete mitogenome sequence of Pseudolaubuca sinensis was determined using PCR amplificationand DNA sequencing, which contains 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a non-coding control region with the total length of 16,617 bp. Except for eight tRNA and ND6 genes, all other mitochondrial genes are encoded on the heavy strand. The codon usage followed the typical vertebrate mitochondrial pattern (ATG or GTG for start codon and TAA or TAG for stop codon). There are 6 regions of gene share totaling 22 bp and 14 intergenic spacer regions totaling 66 bp. The complete mitochondrial genome sequence is useful for phylogenetic analysis and studies of population genetics of Pseudolaubuca sinensis. PMID:25714142

  2. Complete mitochondrial genome of Acrossocheilus yunnanensis (Cypriniformes, Barbinae, Acrossocheilus).

    Science.gov (United States)

    Wu, Jiayun; Huang, Shiqi; Du, Zongjun; Xie, Meng; Zhu, Guangxiang; Wang, Qin; Jiang, Yanzhi; He, Tao; Ren, Hongmei; Zhang, Yi; Wen, Anxiang

    2016-07-01

    In this paper, the complete mitochondrial DNA sequence of Acrossocheilus yunnanensis collected from the Qingyi River is determined. The complete mitochondrial genome of A. yunnanensis is a circular molecule of 16,596 bp in length, and all genes showed the typical gene arrangement conforming to the vertebrate consensus. The 13 protein-coding genes of A. yunnanensis and other 21 Barbinae species from 3 genera were used for phylogenetic analysis using Bayesian inference and maximum likelihood methods. The topology demonstrated the genus Acrossocheilus is relatively close to the Labeo than Garra, the A. yunnanensis may have a common ancestor with A. monticola, where both belong to the non-barred group. PMID:26024134

  3. The complete mitochondrial genome of Sinibotia robusta (Cypriniformes: Cobitidae).

    Science.gov (United States)

    Yu, Peng; Ding, Shuquan; Yang, Qichao; Li, Xilei; Wan, Quan

    2016-09-01

    The Sinibotia robusta mitochondrial genome (GenBank accession no. KP979711) was a circular molecule of 16 575 bp in length, with two rRNA genes, 13 protein-coding genes, 22 tRNA genes, an l-strand replication origin (OL), and a control region (D-loop). The nucleotide acid composition of the entire mitogenome was 31.91% for A, 26.90% for C, 15.56% for G, and 25.63% for T, with an A + T content of 57.54%. And the A + T content of 12S rRNA, 16S rRNA, and D-loop was 51.26%, 56.17%, and 67.73%, respectively. PMID:26258519

  4. Complete mitochondrial genome of Gnathopogon taeniellus (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Ai, Wei-Ming; Liu, Xiao-Xiang; Yuan, Le-Yang; Lu, Yi-Wei; Jiang, Xia-Min

    2016-09-01

    The complete mitochondrial genome of Gnathopogon taeniellus was first determined in this study. It is 16,596 bp in length, contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and one control region in the typical vertebrate gene order and transcriptional direction. There are total of 29 bp short overlaps and 34 bp non-coding intergenic spacers were found in the mitogenome. Overall nucleotide base composition of light strand is 29.3% A, 25.8% C, 17.9% G and 27.0% T. Two start codons (ATG and GTG) and two stop codons (TAG and TAA/T) were used in the protein-coding genes. The origin of light strand replication (OL) was identified between tRNA(Asn) and tRNA(Cys) genes and could fold a hairpin structure. The nucleotide composition of control region is 31.8% A, 20.9% C, 14.0% G and 33.3% T. PMID:25714152

  5. The complete mitochondrial genome of Ambastaia sidthimunki (Cypriniformes: Cobitidae).

    Science.gov (United States)

    Yu, Peng; Wei, Min; Yang, Qichao; Yang, Yingming; Wan, Quan

    2016-09-01

    Ambastaia sidthimunki is a beautiful small-sized fish and it was categorized as Endangered B2ab (iii,v) in the IUCN Red List. In this study, we reported the complete mitochondrial genome of the A. sidthimunki. The mitochondrial genome sequence was a circular molecule with 16,574 bp in length, and it contained 2 ribosomal RNA genes, 22 transfer RNA genes, 13 protein-coding genes, an L-strand replication origin (OL) and a control region (D-loop). The nucleotide acid composition of the entire mitogenome was 26.94% for C, 15.55% for G, 31.84% for A and 25.67% for T, with an AT content of 57.51%. This research contributes new molecular data for the conservation of this Endangered species. PMID:25722037

  6. The complete mitochondrial genome sequence of Schizopygopsis anteroventris (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Liang, Yangyang; Chen, Yifeng; Li, Chunhua; He, Dekui

    2016-09-01

    Schizopygopsis anteroventris (Cyprinidae: Schizothoracinae) is an ecologically and economically important cyprinid endemic to Qinghai-Tibet Plateau, China. In this study, we sequenced the complete mitochondrial genome of S. anteroventris by DNA sequencing based on PCR fragments. The mitogenome of S. anteroventris is 16,620 in length, containing 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and two non-coding regions: the control region (D-loop) and the origin of light-strand replication (OL). The gene order in the mitogenome is identical with common vertebrate form. The complete mitogenome sequence is useful for further genetic studies, phylogenetic analysis and resource protection of S. anteroventris. PMID:25791361

  7. Complete mitochodrial genome of Vanmanenia pingchowensis (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Liang, Zhiqiang; Wang, Chongrui; Wu, Yuanan; Li, Hong; Yuan, Xiping; Wei, Qiwei

    2016-05-01

    The complete mitochondrial genome of Vanmanenia pingchowensis was determined in this study. The gene composition, arrangement and transcriptional orientation in V. pingchowensis mitogenome were identical to most vertebrates. The complete mitogenome of V. pingchowensis was 15,560 bp in size with 13 protein coding genes, 22 tRNA genes, and a control region. Two start codon patterns and three stop codon patterns were found in protein-coding genes. Only the tRNA-Ser2 could not fold into a typical clover-leaf secondary structure for lacking the dihydrouridine arm. Sequences alignment results suggest that the complete mitogenome of V. pingchowensis is an efficient tool to study molecular phylogenetics, biogeography and adaptive evolution of this lineage. PMID:25427810

  8. Complete mitochodrial genome of Parasinilabeo assimilis (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Wu, Yuanan; Liu, Xunhua; Hong, Bo; Li, Hong; Ge, Hongzi; Liang, Zhiqiang; Yuan, Xiping

    2016-05-01

    The complete mitochondrial genome of Parasinilabeo assimilis has been determined in this study. The gene composition, arrangement and transcriptional orientation in P. assimilis mitogenome were identical to most vertebrates. The complete mitogenome of P. assimilis was 16,602 bp in size with 13 protein coding genes, 22 tRNA genes and a control region. Two start codon patterns and three stop codon patterns were found in protein-coding genes. Only the tRNA-Ser2 could not fold into a typical clover-leaf secondary structure due to the lack of the dihydrouridine arm. Sequence alignment results suggest that the complete mitogenome of P. assimilis is an efficient tool to study molecular phylogenetics, biogeography and adaptive evolution of this lineage. PMID:25423522

  9. Complete mitochodrial genome of Bangana decora (Cypriniformes, Cyprinidae).

    Science.gov (United States)

    Li, Hong; Li, Chuanwu; Tang, Xiangbei; Liao, Fuchu; Wang, Chongrui; Liang, Zhiqiang; Liu, Hong; Yuan, Xiping

    2016-05-01

    The complete mitochondrial genome of Bangana decora was determined in this study. The gene composition, arrangement and transcriptional orientation in B. decora mitogenome were identical to most vertebrates. The complete mitogenome of B. decora was 16,607 bp in size with 13 protein-coding genes, 22 tRNA genes and a control region. Two start codon patterns and three stop codon patterns were found in protein-coding genes. Only the tRNA-Ser2 could not fold into a typical clover-leaf secondary structure due to the absence of the dihydrouridine arm. Sequence alignment results suggest that the complete mitogenome of B. decora is an efficient tool to study molecular phylogenetics, biogeography and adaptive evolution of this lineage. PMID:25423524

  10. The complete mitochondrial genome of Gobiobotia filifer (Teleostei, Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Li, Qiang; Liu, Ya; Zhou, Jian; Gong, Quan; Li, Hua; Lai, Jiansheng; Li, Lianman

    2016-09-01

    The Gobiobotia filifer is a small economic fish which distributes in the upstream of Yangtze River and its distributaries. For the environmental pollution and overfishing, its population declined drastically in recent decades, so it is essential to protect its resource. In this study, the complete mitochondrial genome sequence of G. filifer was determined with PCR technology, which contains 13 protein-coding genes, 22 tRNA genes, two rRNA genes, and a non-coding control region with the total length of 16,613 bp. The order and composition of genes were similar to most of the other teleost fish. Most of the genes were encoded on heavy strand, except for ND6 genes and eight tRNAs. Just like most other vertebrates, the bias of G and C has been found in different genes/regions. The complete mitochondrial genome sequence of G. filifer would contribute to better understand evolution of this lineage, population genetics, and will help administrative department to make rules and laws to protect this lineage. PMID:25806579

  11. Sublethal exposure to cadmium interferes with cover-seeking behavior of juvenile crayfish, Procambarus clarkii (Girard)

    Energy Technology Data Exchange (ETDEWEB)

    Misra, R.; Antonelli, J.; Misra, K.; Steele, C.; Skinner, C. [Edinboro Univ., PA (United States). Dept. of Biology and Health Services

    1996-12-31

    The behavioral effects of heavy metals on crayfishes may significantly affect their survival in the environment. Changes in their ability to remain under cover could substantially decrease their survivorship due to increased predation. The effect of sublethal cadmium exposure on the ability of juvenile crayfish to remain in cover was evaluated. Four different treatment groups wee used: a control group and three experimental groups exposed to 1, 2, or 3 mg Cd/L for 7 d. Crayfish were placed, individually, into glass aquaria containing 3 L of laboratory water pre-treated to detoxify all heavy metals, with continuous aeration. Each crayfish was provided with a dark, thigmotactic shelter. Cadmium was introduced into the aquaria on days 1 and 4 to maintain the nominal concentrations. Beginning on day 5 and continuing through day 7, observations were taken on each crayfish five times per day, with a minimum of 30 minutes between observations. Crayfish position was recorded as in cover or in the open area of an aquarium. Juveniles in the control groups were in cover 78.35 of the observations. Over the 3 d of observations, juveniles in the 1 mg Cd/L exposure groups used cover 72.1%. Those in the 2 and 3 mg Cd/L groups used cover 53.9% and 60%, respectively, indicating hyperactivity induced by cadmium exposure. Examining the daily results, however, those juveniles in the 1 mg Cd/L group were in cover only 60% of the time by day 7, indicating a latency to produce hyperactivity at this concentration.

  12. Synthesis, spectroscopic characterization and electrochemical studies of Girard's T chromone complexes

    Science.gov (United States)

    Al-Saeedi, Sameerah I.; Alaghaz, Abdel-Nasser M. A.; Ammar, Reda A.

    2016-05-01

    Complexes of cobalt(II), nickel(II), copper(II) and zinc(II) of general composition [M(L)2] have been. The elemental analyses, molar conductance, spectral, magnetic moment and thermal measurements studies of the compounds led to the conclusion that the ligand acts as a tridentate manner (OON). The molar conductance of the metal complexes in fresh solution of DMSO lies in the range of 8.10-10.18 Ω-1 cm2 mol-1 indicating their non-electrolytic behavior. On the basis of analytical and spectroscopic techniques, octahedral geometry of the complexes was proposed. The ligand field parameters were calculated for Co(II), Ni(II) and Cu(II) complexes and their values were found in the range reported for a octahedral structure. The data show that the complexes have composition of ML2 type. The activation of thermodynamic parameters are calculated using different equations. The octahedral geometry of the complexes is confirmed using DFT method from DMOL3 calculations and ligand field parameters. The cyclic voltammograms of the Cu(II)/Co(II)/Ni(II) complexes investigated in DMSO solution exhibit metal centered electroactivity in the potential range -1.5 to +1.5 V. The electrochemical data obtained for Cu(II) complexes explains the change of structural arrangement of the ligand around Cu(II) ions.

  13. [The salivary glands of Philodryas patagoniensis Girard, 1857 (Serpentes, Colubridae). A morphological, morphometric and histological study].

    Science.gov (United States)

    Lopes, R A; Contrera, M G; da Costa, J R; Petenusci, S O; Lima-Verde, J S

    1982-01-01

    Morphological, morphometrical and histochemical studies of the cell types in the salivary glands of Philodryas patagoniensis have been performed. It is concluded: 1) the acini of supra, infralabial and premaxillary glands are formed by mucous and mucoserous cells; the Duvernoy's gland by seromucous cells; 2) mucous cells show neutral and sulphated mucosubstances and sialic acid; mucoserous cells show neutral mucosubstance, sialic acid and protein radicals; seromucous cells of Duvernoy's gland show neutral mucosubstance and protein radicals. The acinar area, height of tubule and duct cells, and nuclear volume of acinar, tubule and duct cells were evaluated morphometrically. PMID:7181506

  14. Marketing y responsabilidad. Una aproximación ética desde René Girard

    OpenAIRE

    Gómez-Ferrer Lozano, Guillermo

    2016-01-01

    La presente tesis tiene como objeto analizar la ética aplicada al marketing desde la perspectiva de la responsabilidad bajo el presupuesto de la hermenéutica crítica. La ética del marketing supone el descubrimiento y análisis de los principios, valores y fundamentos éticos en el campo del marketing y su función dentro de las organizaciones, así como las responsabilidades del mismo en sus ámbitos de acción e influencia. Para ello, hemos respondido en la primera parte, a qué es el marketing...

  15. Penetration and distribution of carbon particles in a teleost fish, Poecilia formosa (Girard), the Amazon molly

    Energy Technology Data Exchange (ETDEWEB)

    Woodhead, A.D.

    1981-01-01

    Data is given on the tissue distribution of carbon particles in Amazon mollies exposed for periods of 24-120 h to a 0-0.05% suspension. Very little carbon penetrated the body, either by way of the intestine or the gills. The little amount that entered appeared to pass almost exclusively through the posterior intestine. There was no anatomical specialization of the cells in this area equivalent to Peyer's patches in the intestine of mammals. We suggest that the uptake in the posterior intestine is related to its greater surface area and possibly to some enhanced mobility. After intraperitoneal (ip) injection, carbon that entered the body accumulated at three sites: the heart, the mesentery and, especially, in the head kidney. By 120 h large accumulations were seen in the intertubular areas of the kidney; there was no evidence of excretion of carbon particles from these areas. (JMT)

  16. Life history pattern of mosquitofish Gambusia holbrooki (Girard, 1859) in the Tajan River (Southern Caspian Sea to Iran)

    Institute of Scientific and Technical Information of China (English)

    PATIMAR Rahman; GHORBANI Mohsen; GOL-MOHAMMADI Ali; AZIMI-GLUGAHI Hoda

    2011-01-01

    Investigation of the ecology of introduced species in new habitats can allow determinations of the degree, direction, and rate of evolutionary change. The introduction of Gambusia holbrooki in the southern Caspian Sea presents such a situation. We evaluated the life history traits of mosquitofish in the Tajan River basin. A total of 744 G. holbrooki specimens were collected between January and December 2008. The maximum observed ages are 0+ years for males and 1+ years for females. Both sexes grew allometrically (negative for males: b=2.442 and positive for females: 6=3.232). The overall sex ratio is unbalanced and dominated by females. GSI values suggest that this population of G. holbrooki matures between February-July. The highest mean GSI value is 1.80 for males and 15.97 for females in May. Egg diameter ranges from 1.00 to 3.00 mm with a mean value of 2.098 mm. Absolute fecundity varied from 7 to 57 eggs. Both ova diameter and absolute fecundity were positively correlated to fish size (length and weight). Fecundity relative to total weight fluctuated from 34.44 to 582.64 eggs/g, and to total length from 2.33 to 12.95 eggs/cm. Both were negatively correlated with female size. The characteristics of this population are important with respect to life history of the species.

  17. Synthesis, characterization, molecular modeling and antioxidant activity of Girard's T thiosemicarbazide and its complexes with some transition metal ions.

    Science.gov (United States)

    El-Gammal, O A; Mostafa, M M

    2014-06-01

    The chelation behavior of N-{[(allylamino) thiomethyl] hydrazinocarbonylmethyl} trimethylammonium chloride (H3ATHC) towards VO(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+) and UO2(2+) ions have been studied. The structures of the complexes were elucidated using elemental analyses, spectral (IR, UV-visible, (1)H NMR and ESR and mass) as well as magnetic and thermal measurements. The ligand acted as ON bidentate, ONS tridentate donor forming mononuclear complexes. A tetrahedral geometry for Co(2+), square-planar for Ni(2+) and Cu(2+), an octahedral for Zn(2+) and a square-pyramidal arrangement for VO(2+) complexes were proposed, respectively. The EPR spectra of Cu(2+) and VO(2+) complexes confirmed the suggested geometries with values of α(2) and β(2) indicating that the in-plane σ-bonding and in-plane π-bonding are appreciably covalent, and were consistent with very strong in-plane σ bonding in the complexes. Also, the bond length, bond angle, HOMO, LUMO, dipole moment and charges on the atoms have been calculated. Also, the thermal behavior and kinetic parameters were determined using Coats-Redfern and Horowitz-Metzger methods. Furthermore, the synthesized compounds were screened for their superoxide-scavenging activity in the PMS/NADH-NBT system as well as their scavenging effect on ABTS (2,2'-azino-bis(3-ethyl benzthiazoline-6-sulfonic acid) and 2,2-diphenyl-1-picrylhydrazyl(DPPH) radicals. Among these compounds, the ligand and Zn(2+) complex, exhibited the potent ABTS (2,2'-azino-bis(3-ethyl benzthiazoline-6-sulfonic acid) radical scavenging activity, comparable to that of vitamin C. PMID:24705392

  18. Pattern of distribution of dimethylbenz(a)anthracene in the Amazon molly, Poecilia formosa (Girard), after intraperitoneal injection

    Energy Technology Data Exchange (ETDEWEB)

    Woodhead, A.D.; Bornbusch, A.

    1983-01-01

    The present paper describes the distribution and accumulation labelled dimethylbenz(a)anthracene (DMBA) in the Amazon molly after intraperitoneal injection. The study was made to see whether there was preferential accumulation of the compound in the spleen; the findings showed that this was not the case. Two hours after injection, DMBA was present throughout the body, except in the brain and the ovary. There was enhanced deposition at four sites, in the macrophages of the atrium of the heart and the peritoneum, the liver and the exocrine pancreas. DMBA was taken up by reticuloendothelial macrophages for 78 h after injection, then it was lost. The accumulation and disappearance of radioactive label seen in the liver and pancreatic cells probably represented the pattern of metabolism of the compound. By 400 h after injection there was little DMBA remaining. Label accumulated in the ameloblasts, which secrete the enamel capping of the teeth. 12 references, 3 figures, 1 table.

  19. 78 FR 25066 - Girard Gurgick; Notice of Declaration of Intention and Soliciting Comments, Protests, and/or...

    Science.gov (United States)

    2013-04-29

    ..., Protests, and/or Motions To Intervene Take notice that the following application has been filed with the...-8370, or Email address: Ashish.Desai@ferc.gov . j. Deadline for filing comments, protests, and/or..., and Protests may be filed electronically via the Internet. See 18 CFR 385.2001(a)(l)(iii) and...

  20. Evolutionary history of the fish genus Astyanax Baird & Girard (1854 (Actinopterygii, Characidae in Mesoamerica reveals multiple morphological homoplasies

    Directory of Open Access Journals (Sweden)

    Doadrio Ignacio

    2008-12-01

    Full Text Available Abstract Background Mesoamerica is one of the world's most complex biogeographical regions, mostly due to its complex geological history. This complexity has led to interesting biogeographical processes that have resulted in the current diversity and distribution of fauna in the region. The fish genus Astyanax represents a useful model to assess biogeographical hypotheses due to it being one of the most diverse and widely distributed freshwater fish species in the New World. We used mitochondrial and nuclear DNA to evaluate phylogenetic relationships within the genus in Mesoamerica, and to develop historical biogeographical hypotheses to explain its current distribution. Results Analysis of the entire mitochondrial cytochrome b (Cytb gene in 208 individuals from 147 localities and of a subset of individuals for three mitochondrial genes (Cytb, 16 S, and COI and a single nuclear gene (RAG1 yielded similar topologies, recovering six major groups with significant phylogeographic structure. Populations from North America and Upper Central America formed a monophyletic group, while Middle Central America showed evidence of rapid radiation with incompletely resolved relationships. Lower Central America lineages showed a fragmented structure, with geographically restricted taxa showing high levels of molecular divergence. All Bramocharax samples grouped with their sympatric Astyanax lineages (in some cases even with allopatric Astyanax populations, with less than 1% divergence between them. These results suggest a homoplasic nature to the trophic specializations associated with Bramocharax ecomorphs, which seem to have arisen independently in different Astyanax lineages. We observed higher taxonomic diversity compared to previous phylogenetic studies of the Astyanax genus. Colonization of Mesoamerica by Astyanax before the final closure of the Isthmus of Panama (3.3 Mya explains the deep level of divergence detected in Lower Central America. The colonization of Upper Mesoamerica apparently occurred by two independent routes, with lineage turnover over a large part of the region. Conclusion Our results support multiple, independent origins of morphological traits in Astyanax, whereby the morphotype associated with Bramocharax represents a recurrent trophic adaptation. Molecular clock estimates indicate that Astyanax was present in Mesoamerica during the Miocene (~8 Mya, which implies the existence of an incipient land-bridge connecting South America and Central America before the final closure of the Isthmus of Panama (~3.3 Mya.

  1. Life history pattern of mosquitofish Gambusia holbrooki (Girard, 1859) in the Tajan River (Southern Caspian Sea to Iran)

    Science.gov (United States)

    Patimar, Rahman; Ghorbani, Mohsen; Gol-Mohammadi, Ali; Azimi-Glugahi, Hoda

    2011-01-01

    Investigation of the ecology of introduced species in new habitats can allow determinations of the degree, direction, and rate of evolutionary change. The introduction of Gambusia holbrooki in the southern Caspian Sea presents such a situation. We evaluated the life history traits of mosquitofish in the Tajan River basin. A total of 744 G. holbrooki specimens were collected between January and December 2008. The maximum observed ages are 0+ years for males and 1+ years for females. Both sexes grew allometrically (negative for males: b=2.442 and positive for females: b=3.232). The overall sex ratio is unbalanced and dominated by females. GSI values suggest that this population of G. holbrooki matures between February-July. The highest mean GSI value is 1.80 for males and 15.97 for females in May. Egg diameter ranges from 1.00 to 3.00 mm with a mean value of 2.098 mm. Absolute fecundity varied from 7 to 57 eggs. Both ova diameter and absolute fecundity were positively correlated to fish size (length and weight). Fecundity relative to total weight fluctuated from 34.44 to 582.64 eggs/g, and to total length from 2.33 to 12.95 eggs/cm. Both were negatively correlated with female size. The characteristics of this population are important with respect to life history of the species.

  2. Complete mitochondrial genome of Abramis brama orientalis Berg (cypriniformes, Cyprinidae, Leuciscinae).

    Science.gov (United States)

    Qi, Pengzhi; Guo, Baoying; Zhang, Zhiming; Xie, Congxin; Wu, C W

    2014-10-01

    In this study, we cloned and sequenced the complete mitochondrial genome of Abramis brama orientalis Berg. The genome was 16,610 bp (LR) in length and consisted of 13 protein-coding genes, 22 tRNA genes, 2 ribosomal RNA genes and 2 main non-coding regions [the control region (CR) and the origin of the light strand replication], the gene composition and order of which was similar to those reported from other fish mitochondrial genomes. The overall base composition of the heavy strand was T 26.7%, C 26.5 %, A 30.0% and G 16.8%, with a slight A+T bias of 56.7%. This mitogenome sequence data would play an important role in population genetics and phylogenetic analysis of the Leuciscinae. PMID:23815319

  3. Phylogeny of the Southeast Asian freshwater fish genus Pangio (Cypriniformes, Cobitidae)

    Czech Academy of Sciences Publication Activity Database

    Bohlen, Jörg; Šlechtová, Vendula; Tan, H. H.; Britz, R.

    2011-01-01

    Roč. 61, č. 3 (2011), s. 854-865. ISSN 1055-7903 R&D Projects: GA ČR GA206/05/2556; GA ČR GA206/08/0637; GA AV ČR IAA600450508; GA MŠk LC06073 Institutional research plan: CEZ:AV0Z50450515 Keywords : pangio * eel loaches * phylogeny Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.609, year: 2011

  4. Reproductive biology of Garra regressus and Garra tana (Cypriniformes: Cyprinidae from Lake Tana, Ethiopia

    Directory of Open Access Journals (Sweden)

    Akewake Geremew

    2015-05-01

    Full Text Available The reproductive biology of Garra regressus and Garra tana was investigated by collecting monthly samples (January to December 2006 from the southern Gulf of Lake Tana, where these species are endemic.  Garra regressus has an extended breeding time from April to October while G. tana breeds throughout the year with a peak from March to July.  The mean size at maturity in both the species was not significantly different between the sexes, but G. tana had a significantly lower mean size at maturity than G. regressus in female specimens.  Absolute fecundity estimates for G. regressus ranged from 580.8–1800, while those for G. tana ranged from 538.9–2968.  Egg size frequency distribution revealed that G. regressus is a multiple spawner, while G. tana is a single spawner.  The sex ratio in the total catch of G. regressus was found to be skewed in favour of females (Chi-square, P< 0.05, while those for G. tana was not significantly different from 1:1. The mean size at maturity was not significantly different between the sexes for G. tana. 

  5. Finding of triploid Carassius Gibelio (Bloch, 1780)(Cypriniformes, Cyprinidae), in Turkey

    Czech Academy of Sciences Publication Activity Database

    Kalous, Lukáš; Memis, D.; Bohlen, Jörg

    2004-01-01

    Roč. 28, č. 1 (2004), s. 77-79. ISSN 0399-0974 R&D Projects: GA ČR GA523/02/0460 Institutional research plan: CEZ:AV0Z5045916 Keywords : carassius gibelio * cyprinidae * Turkey Subject RIV: ED - Physiology Impact factor: 0.312, year: 2004

  6. Schistura puncticeps, a new species of loach from Myanmar (Cypriniformes: Nemacheilidae)

    Czech Academy of Sciences Publication Activity Database

    Bohlen, Jörg; Šlechtová, Vendula

    2013-01-01

    Roč. 24, č. 1 (2013), s. 85-92. ISSN 0936-9902 R&D Projects: GA ČR GA206/08/0637 Institutional research plan: CEZ:AV0Z50450515 Keywords : Teleostei Nemacheilidae * basin Subject RIV: EG - Zoology Impact factor: 2.275, year: 2013

  7. Molecular evolution of connective tissue growth factor in Cyprinidae (Teleostei: Cypriniformes)

    Institute of Scientific and Technical Information of China (English)

    Xianghui Kong; Xuzhen Wang; Xiaoni Gan; Junbing Li; Shunping He

    2008-01-01

    Connective tissue growth factor (CTGF) plays an important role in regulation ofcell growth, differentiation, apoptosis and individual development in animals. The study of sequences variation and molecular evolution of CTGF gene across various species of the cyprinid could be helpful for understanding of speciation and gene divergence in this kind of fish. In this study, 19 novel sequences of CTGF gene were obtained from the representative species of the family Cyprinidae using PCR amplification, cloning and sequencing. Phylogenetic relationships of Cyprinidae were reconstructed by neighbor-joining (N J), maximum parsimony (MP), maximum likelihood (ML) and Bayesian method. Oryzias latipes from the family Cyprinodontidae was assigned to be the outgroup taxon. Leuciscini and Barbini were clustered into the monophyletic lineages, respectively, with the high nodal supports. The estimation of the ratio of non-synonymous to synonymous substitution (dN/dS) for the various branches indicated that there stood the different evolution rates between the Leuciscini and the Barbini. With the ratio of dN/dS of the Leuciscini being lower than that of the Barbini, species within the Barbini were demonstrated to be subjected to the relatively less selection pressure and under the relaxable evolution background. A 6 bp indel (insertion/deletion) was found at the 5' end of CTGF gene of Cyprinidae, and this 6 bp deletion only appeared in the Leuciscini, which is a typical characteristic of the Leuciscini and provides evidence for the monophylogeny of the Leuciscini. For the amino acid sequences of CTGF protein, the most variations and indels were distributed in the signal region and IGFBP region of this protein, implying that these variations were correlated with the regulation of the CTGF gene expression and protein activity.

  8. Molecular phylogenetic relationships of Eastern Asian Cyprinidae (Pisces: Cypriniformes) inferred from cytochrome b sequences

    Institute of Scientific and Technical Information of China (English)

    HE; Shunping; LIU; Huanzhang; CHEN; Yiyu; Masayuki; Kuwah

    2004-01-01

    Complete mitochondrial cytochrome b sequences of 54 species, including 18 newly sequenced, were analyzed to infer the phylogenetic relationships within the family Cyprinidae in East Asia. Phylogenetic trees were generated using various tree-building methods, including Neighbor-joining (NJ), Maximum Parsimony (MP) and Maximum Likelihood (ML) methods, with Myxocyprinus asiaticus (family Catostomidae) as the designated outgroup. The results from NJ and ML methods were mostly similar, supporting some existing subfamilies within Cyprinidae as monophyletic, such as Cultrinae, Xenocyprinae and Gobioninae (including Gobiobotinae). However, genera within the subfamily "Danioninae" did not form a monophyletic group. The subfamily Leuciscinae was divided into two unrelated groups: the "Leuciscinae" in East Asia forming as a monophyletic group together with Cultrinae and Xenocyprinae, while the Leuciscinae in Europe, Siberia, and North America as another monophyletic group. The monophyly of subfamily Cyprininae sensu Howes was supported by NJ and ML trees and is basal in the tree. The position of Acheilognathinae, a widely accepted monophyletic group represented by Rhodeus sericeus, was not resolved.

  9. The food of roach, Rutilus rutilus (Actinopterygii: Cypriniformes: Cyprinidae), in a biomanipulated water supply reservoir

    Czech Academy of Sciences Publication Activity Database

    Zapletal, T.; Mareš, J.; Jurajda, Pavel; Všetičková, Lucie

    2014-01-01

    Roč. 44, č. 1 (2014), s. 15-22. ISSN 0137-1592 R&D Projects: GA ČR GBP505/12/G112 Institutional support: RVO:68081766 Keywords : diet analysis * gut * biomanipulation * age groups Subject RIV: EG - Zoology Impact factor: 0.577, year: 2014 http://www.aiep.pl/volumes/2010/5_1/pdf/03_1421_F1.pdf

  10. Identification and phylogenetic analysis of the mitogenome of Sarcocheilichthys parvus Nichols (Cypriniformes: Cyprinidae).

    Science.gov (United States)

    Li, Gaopeng; Li, Wenwen; Lv, Sisi; Li, Chen; Qian, Yaoxuan; Xu, Jie; Lu, Yi; Lin, Yuru; Yang, Pengjie; Lin, Zhihua; Yang, Shoubao

    2016-09-01

    In the present study, the complete mitogenome sequence of a Sarcocheilichthys parvus Nichols was sequenced and identified. It contained 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes and 2 non-coding regions. The phylogenetic analysis showed that the mitogenome sequence could be useful data in the field of systematics and conservation biology for S. parvus Nichols and other aquatic species. PMID:26287583

  11. Modified Method of Metaphase Plates Obtaining for Polyploid Fish Genera Carassius and Cobitis Karyotyping (Actinopterygii, Cypriniformes

    Directory of Open Access Journals (Sweden)

    Pukhtayevych P. P.

    2014-08-01

    Full Text Available Ото- браны и апробированы современные методики получения метафазных пластинок соматических клеток рыб, дающих наибольший эффект при исследовании полиплоидных видов. На основании эмпирических данных в отношении представителей родов Carassius и Cobitis рекомендуется ме- тодика с использованием CoCl2 и колхицина. В статье приведено подробное описание модифи- цированной методики и полученные в результате её применения метафазные пластинки Carassius auratus Linnaeus, 1758, Carassius carassius Linnaeus, 1758 и триплоидной формы Cobitis taenia Linnaeus, 1758.

  12. Genetic diversity of Cobitis spp. (Cypriniformes: Cobitidae) from different drainage areas

    Czech Academy of Sciences Publication Activity Database

    Papoušek, Ivo; Lusková, Věra; Koščo, J.; Lusk, Stanislav; Halačka, Karel; Povž, M.; Šumer, S.

    2008-01-01

    Roč. 57, 1-2 (2008), s. 83-89. ISSN 0139-7893. [Loaches of the genus Cobitis and related genera /3./. Šibenik, 24.09.2006-29.09.2006] R&D Projects: GA MŽP SM/6/3/05 Institutional research plan: CEZ:AV0Z60930519 Keywords : Danubian spined loach * population diversity Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 0.522, year: 2008 http://www.ivb.cz/folia/57/1-2/083-089_MS1291.pdf

  13. A new Eocene catostomid (Teleostei: Cypriniformes) from northeastern China and early divergence of Catostomidae

    Institute of Scientific and Technical Information of China (English)

    CHANG; Mee-mann

    2009-01-01

    A new Eocene catostomid genus and species,Plesiomyxocyprinus arratiae, is described from Huadian, Jilin Province, northeastern China. The materials include a nearly complete skeleton, dozens of pharyngeal bones with teeth, and a number of disarticulated bones. The new articulated specimen is large-sized and deep-bodied, with an estimated standard length of ca. 300 mm and body depth of 156 mm or about half of its standard length. The assignment of the fish to the Catostomidae is based on its falciform pharyngeal bone with one row of numerous (more than 50) compressed teeth, and the bone is much smaller than in cyprinids, especially given the size of the fish. The new fish distinguishes itself from all known catostomids (both extinct and extant) in its long anal fin with four unbranched and 17-18 branched rays, and its extremely short caudal peduncle that is only about one fourth of its depth. Plesiomyxocyprinus arratiae resembles the Eocene-Oligocene transpacific-distributed Amyzon in many general skeletal characters. However, it shows a few characters uniquely shared with the Recent catostomid Myxocyprinus asiaticus. Those include a very long dorsal fin with about 50 branched fin rays, the end of dorsal fin rays being close to the caudal fin base, and anal rays stretching posteriorly beyond the base of caudal fin. It is the first fossil catostomid that shows a close relationship to the endemic Myxocyprinus now living in the Yangtze River and Minjiang River, China. The discovery of Plesiomyxocyprinus arratiae, along with two previously described possible catostomid genera Jianghanichthys and Vasnetzovia, may indicate that the divergence of the Catostomidae started much earlier, in the middle Eocene or earlier, on the western side of the Pacific than on its eastern side.

  14. A new Eocene catostomid (Teleostei: Cypriniformes) from northeastern China and early divergence of Catostomidae

    Institute of Scientific and Technical Information of China (English)

    LIU Juan; CHANG Meemann

    2009-01-01

    A new Eocene catostomid genus and species, Plesiomyxocyprinus arratiae, is described from Huadian, Jilin Province, northeastern China. The materials include a nearly complete skeleton, dozens of pharyngeal bones with teeth, and a number of disarticulated bones. The new articulated specimen is large-sized and deep-bodied, with an estimated standard length of ca. 300 mm and body depth of 156 mm or about half of its standard length. The assignment of the fish to the Catostomidae is based on its falciform pharyngeal bone with one row of numerous (more than 50) compressed teeth, and the bone is much smaller than in cyprinids, especially given the size of the fish. The new fish distinguishes itself from all known catostomids (both extinct and extant) in its long anal fin with four unbranched and 17-18 branched rays, and its extremely short caudal peduncle that is only about one fourth of its depth. Plesiomyxocyprinus arratiae resembles the Eocene-Oligocene transpacific-distributed Amyzon in many general skeletal characters. However, it shows a few characters uniquely shared with the Recent catostomid Myxocyprinus asiaticus. Those include a very long dorsal fin with about 50 branched fin rays, the end of dorsal fin rays being close to the caudal fin base, and anal rays stretching posteriorly beyond the base of caudal fin. It is the first fossil catostomid that shows a close relationship to the endemic Myxocyprinus now living in the Yangtze River and Minjiang River, China. The discovery of Plesiomyxocyprinus arratiae, along with two previously described possible catostomid genera Jianghanichthys and Vasnetzovia, may indicate that the divergence of the Catostomidae started much earlier, in the middle Eocene or earlier, on the western side of the Pacific than on its eastern side.

  15. The mitochondrial genome of Schizothorax esocinus (Cypriniformes: Cyprinidae) from Northern Pakistan.

    Science.gov (United States)

    Fiaz Khan, Muhammad; Nasir Khan Khattak, Muhammad; He, Dekui; Liang, Yangyang; Li, Chunhua; Ullah Dawar, Farman; Chen, Yifeng

    2016-09-01

    The mitochondrial DNA sequence represents a mainstay of phylogenetics. It allows biologists to elucidate the evolutionary relationships among species. It also permits an examination of the relatedness of populations, hence got importance in the field of biology. The current study was designed to know the mitochondrial sequence of Schizothorax esocinus, a delicious fish belonging to family Cyprinidae. In this study, the complete mitochondrial genome of S. esocinus from Northern Pakistan was determined. The mitogenome of S. esocinus was found to be 16 591 bp in length and consisted of 13 protein coding genes, 22 tRNA genes, two rRNA genes, and one control region. All genes were encoded on heavy strain except ND6 and few tRNA genes. The overall base composition was T 25.5%, C 27.0%, A 29.8% and G 17.7%, A + T content 55.3% and G + C content 47.7%. The length of control region (CR) was 938 bp and contained putative termination-associated sequence (TAS) and several conserved sequence blocks (CSB) and control region also contained a microsatellite region (TA). This is the very first study reported from a reservoir of large cold water bodies in Pakistan which have a great potential for conservation of cold water fish species. This mitogenome sequence would be useful to know the phylogenetic status of the fish. PMID:26369993

  16. Plasma testosterone and 11-ketotestosterone levels of male pacu Piaractus mesopotamicus (Cypriniformes, Characidae

    Directory of Open Access Journals (Sweden)

    R. Gazola

    1997-12-01

    Full Text Available The levels of testosterone (T and 11-ketotestosterone (11-KT of the South American pacu Piaractus mesopotamicus were determined by radioimmunoassay during two stages of the reproductive cycle, i.e., resting and maturation, and the gonadosomatic index (GSI was calculated. The highest levels of T and 11-KT were reached during the maturation stage (T = 2400 ± 56 pg/ml; 11-KT = 2300 ± 60 pg/ml and lower levels were maintained during the resting period. The rise in androgen levels occurred with the appearance of spermatozoa in the maturation stage, when GSI was highest

  17. Ploidy differences represent an impassable barrier for hybridization. Is there an exception among botiid loaches (Cypriniformes: Botiidae?

    Directory of Open Access Journals (Sweden)

    Joerg Bohlen

    2015-12-01

    Full Text Available One of the most efficient mechanisms to keep animal lineages separate is a difference in ploidy level, since hybrid offspring from parents with different ploidy level are generally sterile. In the freshwater fish family Botiidae, ploidy difference has been held responsible for the separation of its two subfamilies, the tetraploid Botiinae and the diploid Leptobotiinae. However, the species ‘Botia’ zebra combines a number of morphological characters of a genus of Leptobotiinae with morphological characters of a genus of Botiinae, giving rise to the idea that it is the result of a hybridisation event between two species from different subfamilies. We here present a closer morphological examination, an allozyme comparison between ‘Botia’ zebra and the two potential parental species, a cytogenetic analyses as well as phylogenetic reconstructions basing on the complete mitochondrial cytochrome b gene and on the nuclear RAG-1 gene. The results are discussed in a phylogenetic, geographic and ecologic context.

  18. Genetic variation in Labeo fimbriatus (Cypriniformes: Cyprinidae) populations as revealed by partial cytochrome b sequences of mitochondrial DNA.

    Science.gov (United States)

    Swain, Subrat Kumar; Bej, Dillip; Das, Sofia Priyadarsani; Sahoo, Lakshman; Jayasankar, Pallipuram; Das, Pratap Chandra; Das, Paramananda

    2016-05-01

    Labeo fimbriatus, a medium sized carp is assessed as a commercially important aquaculture species in Indian subcontinent. In the present study, the genetic diversity and population structure of four Indian riverine populations of L. fimbriatus have been evaluated using partial cytochrome b sequences of mitochondrial DNA. Sequencing and analysis of this gene from 108 individuals defined 7 distinct haplotypes. Haplotype diversity (Hd) and nucleotide diversity (π) ranged from 0.067 to 0.405 and 0.00023 to 0.03231, respectively. The Mahanadi population had the highest π level. Analysis of molecular variance (AMOVA) indicated that 47.36% of genetic variation contained within population and 53.76% of genetic variation among groups. Pairwise FST analysis indicated that there was little or no genetic differentiation among populations (-0.0018 to 04572) from different geographical regions except Mahanadi population. The Mahanadi population can be considered as a separate stock from rest three riverine populations. Accordingly, the genetic information generated from this study can be implemented while taking decision in formulating base population for the sustainable selective breeding programs of this species. PMID:25329277

  19. [Morphological changes in gametes of tiger barb Puntius tetrazona (Cypriniformes: Cyprinidae) and the implementation of in vitro fertilization].

    Science.gov (United States)

    Domínguez-Castanedo, Omar; Toledano-Olivares, Ángel; Martínez-Espinosa, David; Ávalos-Rodríguez, Alejandro

    2014-12-01

    The production of ornamental fishes represents an economic activity of a growing number of Mexican families. Nevertheless, the reproduction of fish in captivity is one of the complications faced by farmers. This study was set up to: (i) evaluate the morphological and functional changes induced by hydration in the gametes of fish tiger barb (Puntius tetrazona; 240 samples) at tree times after hydration (10, 20 and 30s) with classic spermograms (volume, sperm concentration, viability, motility, and normal morphology); and (ii) evaluate the implementation of in vitro fertilization based on the ovulation rate, the percentage of fertilization and hatching; and the larval numbers obtained after 72 hours. The average volume of milt was 3.0 ± 0.7 μL, and the minimum, maximum and average concentration of sperm was 44.4 x 10(6) spz/mL, 52.3 x 10(6) spz/mL, and 48.1 ± 5.9 x 10(6) spz/mL, respectively. The viability and motility of the sperm was 84.6 ± 3.2% and 81.5 ± 2.2%, respectively. The diameter of the sperm with/without water contact was 2.10 ± 6 μm and 3.8 ± 1.0 μm (p fish as a model for other ornamental fishes of commercial interest. Our results demonstrate that the in vitro fertilization is a very high viable option to optimize and maximize resources; besides, the reproduction management optimization under controlled conditions may enhance wild fish stocks preservation. PMID:25720172

  20. Sex ratio and body size in Cobitis elongatoides and Sabanejewia balcanica (Cypriniformes; Cobitidae) from a thermal spring

    Czech Academy of Sciences Publication Activity Database

    Bohlen, Jörg; Freyhof, J.; Nolte, A.

    2008-01-01

    Roč. 57, 1-2 (2008), s. 191-197. ISSN 0139-7893. [International Conference Loaches of the genus Cobitis and related genera. Šibenik, 24.09.2006-29.09.2006] R&D Projects: GA ČR GA206/05/2556; GA AV ČR IAA600450508 Institutional research plan: CEZ:AV0Z50450515 Keywords : environmental sex determination * Fisher's principle * cobitine loach Subject RIV: EG - Zoology Impact factor: 0.522, year: 2008

  1. Taxonomic status of a population of Gymoncypris waddelli Regan, 1905 (Cypriniformes: Schizothoracinae) distributed in Pengqu River, Tibet, China.

    Science.gov (United States)

    Tang, Yong-Tao; Feng, Chen-Guang; Wanghe, Kun-Yuan; Li, Guo-Gang; Zhao, Kai

    2016-01-01

    Gymnocypris waddelli, a highland cold-water fish, is distributed among rivers and lakes of the southern Qinghai-Tibet Plateau. Although previously described as G. waddelli, specimens from the Pengqu River are morphologically distinguishable from those from Lake Yamzhog Yumco, which is the type locality of G. waddelli. We investigated morphological variations of G. waddelli specimens from Pengqu River and Lake Yamzhog Yumco using not only traditional morphological methods but also newly-developed geometric morphometric analysis; furthermore, mitochondrial Cytochrome b (Cyt b) gene analysis was employed to explore the phylogenetic position of the Pengqu River population. Our morphological analyses suggested that G. waddelli from the Pengqu River had an obtuse snout, nearly straight oral fissure, shorter lower jaw than upper jaw, broad mouth and sparse inside gill rakers of the first arch relative to the Lake Yamzhog Yumco population. The strongly supported monophyly of Pengqu population according to mitochondrial datasets was obtained, indicating genetic differences between Pengqu River and Lake Yamzhog Yumco populations. Given their substantial genetic and morphological divergence, specimens from Pengqu River warrant recognition as a distinct species, here described and named Gymnocypris pengquensis. PMID:27395577

  2. Garra arupi, a new cyprinid fish species (Cypriniformes: Cyprinidae from upper Brahmaputra basin in Arunachal Pradesh, India

    Directory of Open Access Journals (Sweden)

    K. Nebeshwar

    2009-04-01

    Full Text Available Garra arupi, a new cyprinid fish species is differentiated from its congeners along the base of the Himalaya in Brahmaputra basin by a combination of characters: two pairs of barbels, anterior position of vent (vent to anal distance 52.6-60.0% pelvic to anal distance, a band of 6-9 prominent horny tubercles on the tip of the snout, a submarginal black band on the dorsal fin, and 16 circumpeduncular scales. It is similar to G. kempi in having an anterior position of the vent, and similar to G. lissorynchus in having a distinct submarginal band on the dorsal fin. Garra arupi differs from G. kempi in having 35-36 (vs. 40-42 lateral line scales, 11-12 (vs. 13-14 predorsal scales, 16 (vs. 12 circumpeduncular scales, the presence of a distinct submarginal band on the dorsal fin (vs. absence, the presence of a transverse band of tubercles on the snout tip (vs. absence, and the presence of 7-8 thin stripes on the caudal peduncle (vs. absence. Garra arupi differs from G. lissorynchus in having 11-12 (vs. 14-15 predorsal scales, 7 (vs. 6 branched dorsal fin rays, 5 (vs. 4 branched anal fin rays, the absence of a W-shaped color band on the caudal fin (vs. presence, the absence of a rostral lobe on the snout (vs. presence, the presence of a transverse band of tubercles on the snout tip (vs. absence, and a vent to anal distance 52.6-60.0 (vs. 37.3-40.2 % pelvic to anal distance.

  3. An invaded invader: high prevalence of entocytherid ostracods on the red swamp crayfish Procambarus clarkii (Girard, 1852) in the Eastern Iberian Peninsula

    Czech Academy of Sciences Publication Activity Database

    Aguilar-Alberola, J. A.; Mesquita-Joanes, F.; López, S.; Mestre, A.; Casanova, J. C.; Rueda, J.; Ribas, Alexis

    2012-01-01

    Roč. 688, č. 1 (2012), s. 63-73. ISSN 0018-8158 Institutional research plan: CEZ:AV0Z60930519 Keywords : Ostracoda * Commensalism * Alien species * Biological invasion Subject RIV: EG - Zoology Impact factor: 1.985, year: 2012

  4. Fanny CHABROL et Gabriel GIRARD (dirs.), VIH/sida. Se confronter au terrain. Expériences et postures de recherche

    OpenAIRE

    Farnarier, Cyril

    2011-01-01

    Résultant de journées doctorales, organisées en avril 2008, VIH/sida. Se confronter au terrain. Expériences et postures de recherche est publié par l’ANRS et disponible en téléchargement gratuit sur le site de l’Agence. Affichant la volonté de « produire des données utiles pour tous les acteurs impliqués dans la lutte conte le VIH/sida » cet ouvrage ne s’adresse donc pas seulement aux chercheurs en sciences sociales. Une attention particulière doit alors être portée au lien extrêmement fort q...

  5. Sylvain Boulouque et Pascal Girard (dir.), Traîtres et trahisons. Guerres, imaginaires sociaux et constructions politiques, Préface de Philippe Buton

    OpenAIRE

    Panel, Louis

    2008-01-01

    Les actes de ce colloque tenu à Troyes les 14 et 15 octobre 2005 rassemblent des contributions originales sur les diverses formes et perceptions de la trahison, essentiellement en France. La première cession intitulée « territoires et couleurs de la trahison » traite d’abord des figures du traître dans le mouvement républicain irlandaisavant d’étudier la naissance du mot et du concept de « jaunes » dans le mouvement ouvrier français. Une deuxième partie, consacrée à la trahison en temps de gu...

  6. [Effect of food availability in early ontogenesis on the rate of growth and numbers of bream Abramis brama L. (Cypriniformes, Cyprinidae) in Kursh Bay of the Baltic Sea].

    Science.gov (United States)

    Naumenko, E N

    2011-01-01

    On the basis of studies of zooplankton and ichthyoplankton in the coastal zone of Kursh Bay of the Baltic Sea, zooplankton production accessible for juveniles of bream Abramis brama L. at early stages of ontogenesis, degree of removal of food resources, and food availability of juveniles of the bream that was reflected in the rate of growth were calculated. It is concluded that the removal of more than 60% of production of food zooplankton by bream juveniles leads to an increase of their mortality at early stages and a decrease in the rate of increase in the body weight. PMID:21786657

  7. Phylogeography of Pteronotropis signipinnis, P. euryzonus, and the P. hypselopterus Complex (Teleostei: Cypriniformes, with Comments on Diversity and History of the Gulf and Atlantic Coastal Streams

    Directory of Open Access Journals (Sweden)

    Richard L. Mayden

    2015-01-01

    Full Text Available The cyprinid genus Pteronotropis is endemic to southeastern Gulf of Mexico and Atlantic Ocean of North America. Never before has the genus been demonstrated to be monophyletic. We investigate both the phylogenetic relationships and the phylogeography of some species in the genus using mitochondrial ND2 sequences. In no analysis is the genus resolved as monophyletic if Notropis harperi is not included in the genus. Biogeographic and phylogeographic evaluations are conducted with Pteronotropis, including P. signipinnis, P. euryzonus, and the P. hypselopterus complex. Patterns of relationships and population genetic analyses support divergences within multiple clades both at the species level and within species that are tied to abiotic changes in the region. Replicated patterns across clades are observed, as well as patterns previously found in other taxa. Pteronotropis hypselopterus is likely not a natural grouping as populations from some drainages form clades more closely related to other species of the genus. The general patterns of relationships indicate likely cryptic species not currently recognized. Finally, the patterns of species relationships and clades and population structuring within species serve as another example of replicated divergences in the biodiversity east and west of the Mobile Bay.

  8. Phylogeography of Pteronotropis signipinnis, P. euryzonus, and the P. hypselopterus Complex (Teleostei: Cypriniformes), with Comments on Diversity and History of the Gulf and Atlantic Coastal Streams.

    Science.gov (United States)

    Mayden, Richard L; Allen, Jason

    2015-01-01

    The cyprinid genus Pteronotropis is endemic to southeastern Gulf of Mexico and Atlantic Ocean of North America. Never before has the genus been demonstrated to be monophyletic. We investigate both the phylogenetic relationships and the phylogeography of some species in the genus using mitochondrial ND2 sequences. In no analysis is the genus resolved as monophyletic if Notropis harperi is not included in the genus. Biogeographic and phylogeographic evaluations are conducted with Pteronotropis, including P. signipinnis, P. euryzonus, and the P. hypselopterus complex. Patterns of relationships and population genetic analyses support divergences within multiple clades both at the species level and within species that are tied to abiotic changes in the region. Replicated patterns across clades are observed, as well as patterns previously found in other taxa. Pteronotropis hypselopterus is likely not a natural grouping as populations from some drainages form clades more closely related to other species of the genus. The general patterns of relationships indicate likely cryptic species not currently recognized. Finally, the patterns of species relationships and clades and population structuring within species serve as another example of replicated divergences in the biodiversity east and west of the Mobile Bay. PMID:26114110

  9. Different Histories, Different Destinies‒Impact of Evolutionary History and Population Genetic Structure on Extinction Risk of the Adriatic Spined Loaches (Genus Cobitis; Cypriniformes, Actinopterygii.

    Directory of Open Access Journals (Sweden)

    Ivana Buj

    Full Text Available The region of Balkans is often considered as an ichthyologic "hot spot", with a great number of species and high portion of endemics living in fresh waters in a relatively small area. The Adriatic watershed in Croatia and Herzegovina is inhabited by six spined loach species (genus Cobitis whose extinction risk estimations were based solely on their extent of occurrence (and/or area of occupancy and its fragmentation, and conservation proposals do not consider diversity below species level. In this investigation we employed molecular genetic methods to describe present genetic structure of the Adriatic spined loaches and reveal their demographic history. The divergence of the Adriatic lineages inside the genus Cobitis started in Miocene and lasted until Pleistocene epoch. Geological events responsible for shaping recent diversity of spined loaches in the Adriatic basin are: the Dinarid Mountains upwelling, the evolution of Dinaric Lake system, local tectonic activity, river connections during glaciations and differences in sea level. Even though all the investigated species inhabit karstic rivers located in the same geographic area and that were subject of similar geological events, the results obtained reveal great differences in their genetic diversity and structure and point out the necessity of different conservation measures to ensure their future viability. High level of genetic polymorphism is characteristic for species located more to the south. Two species comprised of more than one population have completely different intraspecific structure; populations of C. illyrica are genetically distinct and represent separate evolutionary significant units, whereas intraspecific structure of C. narentana corresponds to metapopulational pattern. Without population genetic data, evolutionary significant units could be easily misidentified. Furthermore, the obtained results affirm that population genetic measurements are able to detect differences among closely located and related species and estimate extinction risk even more accurately than currently applied IUCN criteria.

  10. Different Histories, Different Destinies‒Impact of Evolutionary History and Population Genetic Structure on Extinction Risk of the Adriatic Spined Loaches (Genus Cobitis; Cypriniformes, Actinopterygii).

    Science.gov (United States)

    Buj, Ivana; Ćaleta, Marko; Marčić, Zoran; Šanda, Radek; Vukić, Jasna; Mrakovčić, Milorad

    2015-01-01

    The region of Balkans is often considered as an ichthyologic "hot spot", with a great number of species and high portion of endemics living in fresh waters in a relatively small area. The Adriatic watershed in Croatia and Herzegovina is inhabited by six spined loach species (genus Cobitis) whose extinction risk estimations were based solely on their extent of occurrence (and/or area of occupancy) and its fragmentation, and conservation proposals do not consider diversity below species level. In this investigation we employed molecular genetic methods to describe present genetic structure of the Adriatic spined loaches and reveal their demographic history. The divergence of the Adriatic lineages inside the genus Cobitis started in Miocene and lasted until Pleistocene epoch. Geological events responsible for shaping recent diversity of spined loaches in the Adriatic basin are: the Dinarid Mountains upwelling, the evolution of Dinaric Lake system, local tectonic activity, river connections during glaciations and differences in sea level. Even though all the investigated species inhabit karstic rivers located in the same geographic area and that were subject of similar geological events, the results obtained reveal great differences in their genetic diversity and structure and point out the necessity of different conservation measures to ensure their future viability. High level of genetic polymorphism is characteristic for species located more to the south. Two species comprised of more than one population have completely different intraspecific structure; populations of C. illyrica are genetically distinct and represent separate evolutionary significant units, whereas intraspecific structure of C. narentana corresponds to metapopulational pattern. Without population genetic data, evolutionary significant units could be easily misidentified. Furthermore, the obtained results affirm that population genetic measurements are able to detect differences among closely located and related species and estimate extinction risk even more accurately than currently applied IUCN criteria. PMID:26176227

  11. Asexual Reproduction Does Not Apparently Increase the Rate of Chromosomal Evolution: Karyotype Stability in Diploid and Triploid Clonal Hybrid Fish (Cobitis, Cypriniformes, Teleostei)

    Science.gov (United States)

    Majtánová, Zuzana; Choleva, Lukáš; Symonová, Radka; Ráb, Petr; Kotusz, Jan; Pekárik, Ladislav; Janko, Karel

    2016-01-01

    Interspecific hybridization, polyploidization and transitions from sexuality to asexuality considerably affect organismal genomes. Especially the last mentioned process has been assumed to play a significant role in the initiation of chromosomal rearrangements, causing increased rates of karyotype evolution. We used cytogenetic analysis and molecular dating of cladogenetic events to compare the rate of changes of chromosome morphology and karyotype in asexually and sexually reproducing counterparts in European spined loach fish (Cobitis). We studied metaphases of three sexually reproducing species and their diploid and polyploid hybrid clones of different age of origin. The material includes artificial F1 hybrid strains, representatives of lineage originated in Holocene epoch, and also individuals of an oldest known age to date (roughly 0.37 MYA). Thereafter we applied GISH technique as a marker to differentiate parental chromosomal sets in hybrids. Although the sexual species accumulated remarkable chromosomal rearrangements after their speciation, we observed no differences in chromosome numbers and/or morphology among karyotypes of asexual hybrids. These hybrids possess chromosome sets originating from respective parental species with no cytogenetically detectable recombinations, suggesting their integrity even in a long term. The switch to asexual reproduction thus did not provoke any significant acceleration of the rate of chromosomal evolution in Cobitis. Asexual animals described in other case studies reproduce ameiotically, while Cobitis hybrids described here produce eggs likely through modified meiosis. Therefore, our findings indicate that the effect of asexuality on the rate of chromosomal change may be context-dependent rather than universal and related to particular type of asexual reproduction. PMID:26808475

  12. Genetic variation of wild and hatchery populations of the catla Indian major carp (Catla catla Hamilton 1822: Cypriniformes, Cyprinidae revealed by RAPD markers

    Directory of Open Access Journals (Sweden)

    S.M. Zakiur Rahman

    2009-01-01

    Full Text Available Genetic variation is a key component for improving a stock through selective breeding programs. Randomly amplified polymorphic DNA (RAPD markers were used to assess genetic variation in three wild population of the catla carp (Catla catla Hamilton 1822 in the Halda, Jamuna and Padma rivers and one hatchery population in Bangladesh. Five decamer random primers were used to amplify RAPD markers from 30 fish from each population. Thirty of the 55 scorable bands were polymorphic, indicating some degree of genetic variation in all the populations. The proportion of polymorphic loci and gene diversity values reflected a relatively higher level of genetic variation in the Halda population. Sixteen of the 30 polymorphic loci showed a significant (p < 0.05, p < 0.01, p < 0.001 departure from homogeneity and the F ST values in the different populations indicated some degree of genetic differentiation in the population pairs. Estimated genetic distances between populations were directly correlated with geographical distances. The unweighted pair group method with averages (UPGMA dendrogram showed two clusters, the Halda population forming one cluster and the other populations the second cluster. Genetic variation of C. catla is a useful trait for developing a good management strategy for maintaining genetic quality of the species.

  13. Families of Cobitoidea (Teleostei; Cypriniformes) as revealed from nuclear genetic data and the position of the mysterious genera Barbucca, Psilorhynchus, Serpenticobitis and Vaillantella

    Czech Academy of Sciences Publication Activity Database

    Šlechtová, Vendula; Bohlen, Jörg; Tan, H. H.

    2007-01-01

    Roč. 44, - (2007), s. 1358-1365. ISSN 1055-7903 R&D Projects: GA ČR GA206/05/2556; GA ČR GA206/00/0668; GA AV ČR IAA600450508; GA MŠk LC06073 Institutional research plan: CEZ:AV0Z50450515 Keywords : Cobitoidea Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.994, year: 2007

  14. The complete mitochondrial genome of the Chinese hook snout carp Opsariichthys bidens (Actinopterygii: Cypriniformes) and an alternative pattern of mitogenomic evolution in vertebrate

    DEFF Research Database (Denmark)

    Wang, Xuzhen; Wang, Jun; He, Shunping;

    2007-01-01

    noncoding control region. We use these data and homologous sequence data from multiple other ostariophysan fishes in a phylogenetic evaluation to test hypothesis pertaining to codon usage pattern of O. bidens mitochondrial protein genes as well as to re-examine the ostariophysan phylogeny. The mitochondrial...... genome of O. bidens reveals an alternative pattern of vertebrate mitochondrial evolution. For the mitochondrial protein genes of O. bidens, the most frequently used codon generally ends with either A or C, with C preferred over A for most fourfold degenerate codon families; the relative synonymous codon...

  15. Phylogenetic relationships of Cyprinidae (Teleostei: Cypriniformes) inferred from the partial S6K1 gene sequences and implication of indel sites in intron 1

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    The family Cyprinidae is widely distributed in East Asia, and has the important phylogenetic signifi- cance in the fish evolution. In this study, the 5′ end partial sequences (containing exon 1, exon 2 and indel 1) of S6K1 gene were obtained from 30 representative species in Cyprinidae and outgroup using PCR amplification and sequencing. The phylogenetic relationships of Cyprinidae were reconstructed with neighbor joining (NJ), maximum parsimony (MP), maximum likelihood (ML), and Bayesian meth- ods. Myxocyprinus asiaticus (Catostomidae) was assigned to the outgroup taxon. Similar phylogenetic relationships within the family Cyprinidae were achieved with the four analyses. Leuciscini and Barbini were monophyletic lineages respectively with the high nodal supports. Leuciscini comprises Hypophthalmichthyinae, Xenocyprinae, Cultrinae, Gobioninae, Acheilognathinae and East Asian species of Leuciscinae and Danioninae. Monophyly of East Asian clade was supported with high nodal support. Barbini comprises Schizothoracinae, Barbinae, Cyprininae and Labeoninae. The monophyletic lineage consisting of Danio rerio, D. myersi, and Rasbora trilineata was basal in the tree. In addition, the large fragment indels in intron 1 were analyzed to improve the understanding of Cyprinidae relationships. The results showed that the large fragment indels were correlated with the relations among species. Some conserved regions in intron 1 were thought to be involved in the functional regulation. However, no correlation was found between sequence variations and species characteristic size.

  16. The influence of water hardness and PCBs mixtures on embryonic development and hatching of Prussian carp, Carassius gibelio (Actinopterygii: Cypriniformes: Cyprinidae.

    Directory of Open Access Journals (Sweden)

    Magdalena Katarzyna Socha

    2015-11-01

    After 24 h of incubation there was no significant difference in the percentage of live eggs between groups either in experiment with Aroclor 1254 or Delor 103. In the case of hatching rate tested PCBs mixtures had no effect in both variants of water harness. Delor 103 had no effect on the percentage of deformed larvae of Prussian carp in both hard and soft water. Only the observation of the number of deformed larvae showed higher teratogenic influence of Aroclor 1254 in hard water. Summing up, our results showed that Delor 103 is not harmful to the development of activated Prussian carp eggs in both hard and soft water, but in the case of Aroclor 1254 diluted in hard water increased teratogenic effect was observed.

  17. EARLY DEVELOPMENT OF ZACCO PLATYPUS (CYPRINIFORMES: CYPRINIDAE) DISTRIBUTED IN BEIJING%北京地区宽鳍(鱼巤)的早期发育

    Institute of Scientific and Technical Information of China (English)

    邢迎春; 赵亚辉; 李高岩; 唐文乔; 张春光

    2011-01-01

    Pale chub Zacco platypus (Temminck & Schlegel, 1846), a kind of small cyprinid fish, widely distributes in the East Asia including east part of China, Japan and Korea Peninsula. This fish is always found in rivers and streams, especially in the swiftly running waters. In breeding season, the male will express remarkable sexual dimorphism. Numerous sharp nuptial tubercles can be found on the head (especially jaws) and fins. Recently, the populations of Zacco platypus are shrinking down in many places in China due to over-capture, environment change and pollution. Under this background, understanding the life history of Zacco platypus will be very helpful for future conservation and sustainable use. Therefore, we observed the early development of Zacco platypus collected from the Huaijiuhe River in Beijing. Fertilized eggs were obtained through dry method of artificial fertilization, 10-15 fertilized eggs in the process ofgranule development were randomly selected. The processes and characteristics of early development have been observed and documented under the microscope. The mature eggs were spherical in shape, and the average egg diameter was 1.04 mm. At average water temperature of 23.CC (17.1-28.0"C), the early development from fertilization to hatch took total 73h01m and 1682.31Ch accumulated temperature. The embryonic development took 37min to develop into blastodermic disc through discoidal cleavage; and then took another more than 30min to develop into two-cell. Mulberry occurred about 4h after fertilization, multi-layer stack such as morula blastomeres, animal pole extremely high uplift. Blastula finished at 6h22min after fertilization, and embryonic development was into gastrula about 6hl8min after late blastula. Blastopore formed around 19h after fertilization, and then myocomma appeared when blastopore closed. Eye capsule formed at 22h46min after fertilization. 25h27min after fertilization, tail bud appeared on the ventral posterior embryo. Tail fin formed around 29h after fertilization. The embryonic development took lh to develop into effect of muscle after tail fin, embryo body could be seen tic regular intermittent, spherical yolk sac cylindrical front, rear section with the further growth of the caudal fin was elongate. Heart fully formed and slowly beat at 38h35min after fertilization, average heart beat was 84 times per min. The embryonic development took around 73h from fertilized egg to hatch, and then took 6.3 days to develop into flexion larvae. The development speed was slower in larvae than that in embryonic stage. Flexion larvae died in large scales may be because limited conditions of artificial cultivation and lack of energy supply when coexistence of mixed nutrition. Comparing to 21 other cyprinid species, development time of Zacco platypus was longer than that of seven species. Total development time of Zacco platypus was slightly longer than sympatric species Opsariichthys bidens, and obviously longer than Zacco temminckii. We also found the development speed before hatch of northern population was faster than southern one, but slower after hatch.%作者对分布于北京市怀柔区怀九河的宽鳍(鱼巤)(Zacco platypus)的胚胎发育及仔鱼前期发育进行了研究,过程中采用人工干法授精获取受精卵,观察并描述了宽鳍(鱼巤)早期发育过程及其特点.结果显示,宽鳍(鱼巤)成熟卵呈圆球状,平均卵径1.04 mm,为沉性卵.在平均23.0℃(17.1-28.0℃)水温条件下,从受精卵到孵化经历了73hlmin,积温为1682.3度·时;孵化后6.5d进入弯曲期仔鱼.仔鱼前期发育速度与出膜前相比明显减慢;弯曲期仔鱼出现大量死亡可能与有限的人工培育条件、混合营养期能量供给不足等原因有关.通过比较发现,宽鳍(鱼巤)与鲤科中其他21个种相比,早期发育时间比其中7个种均长.宽鳍(鱼巤)早期发育时间比同域分布、相同发育水温的马口鱼略长,明显长于同属的纵纹(鱼巤)的发育时间.宽鳍(鱼巤)南、北方种群仔鱼发育速度存在差异,北方种群出膜前发育速度比南方种群快,但出膜后发育速度减慢.

  18. Asexual Reproduction Does Not Apparently Increase the Rate of Chromosomal Evolution: Karyotype Stability in Diploid and Triploid Clonal Hybrid Fish (Cobitis, Cypriniformes, Teleostei)

    Czech Academy of Sciences Publication Activity Database

    Majtánová, Zuzana; Choleva, Lukáš; Symonová, Radka; Ráb, Petr; Kotusz, J.; Pekárik, L.; Janko, Karel

    2016-01-01

    Roč. 11, č. 1 (2016), e0146872-e0146872. E-ISSN 1932-6203 R&D Projects: GA ČR GAP506/10/1155; GA ČR GPP506/12/P857; GA ČR GA13-12580S Institutional support: RVO:67985904 Keywords : in-situ hybridization * fresh water fish * unisexual salamanders Subject RIV: EG - Zoology Impact factor: 3.234, year: 2014

  19. Four new records of fish species (Cypriniformes: Nemacheilidae, Balitoridae; Characiformes: Prochilodontidae) and corrections of two misidentified fish species (Tetraodontiformes: Tetraodontidae; Beloniformes: Belonidae) in Yunnan, China.

    Science.gov (United States)

    Endruweit, Marco

    2014-01-01

    In this study, six fish species of five families are reported for the first time from Yunnan Province, China. The nemacheilid Schistura amplizona Kottelat, 2000 is reported from the Luosuojiang River and Nanlahe River subbasins, Mekong basin; the prochilodontid Prochilodus lineatus (Valenciennes, 1837), the balitorid Vanmanenia serrilineata Kottelat, 2000, and the tetraodontid Monotrete turgidus Kottelat, 2000, from Nanlahe River subbasin, Mekong basin; the balitorid Beaufortia daon (Mai, 1978), and the belonid Xenentodon canciloides (Bleeker, 1854), both, from Black River subbasin, Red River basin. The freshwater puffer M. turgidus and the needlefish X. canciloides have been previously misidentified as Tetraodon leiurus (Bleeker, 1950) and Tylosurus strongylurus (van Hasselt, 1823), respectively. PMID:24470454

  20. Microscopia óptica e eletrônica do corpúsculo de Stannius do curimbatá (Prochilodus scrofa Steindachner, 1881 (Osteichthyes, Cypriniformes, Prochilodontidae

    Directory of Open Access Journals (Sweden)

    Elza Ferreira Goldman Pinheiro

    1986-01-01

    Full Text Available The Stannius corpuscles of Prochilodus scrofa are studied microscopically (both optically and electronically as well as macroscopically. Two CS, and sometimes 3 or 4, are found per animal. They are pink-coloured organs, rounded in shape and scattered laterally in the first anterior third of the kidney. Each CS is enveloped by a thick connective tissue capsule. The grandular parenchyma consists of only one cellular (PAS-/AF- type, cylindrical in shape, with oval or rounded nucleus located eccentrically with visible nucleolus. The ultrastructural description is as follows: well developed Golgi complexes, rough endoplasmic reticulum and mitochondria in moderate quantities. Electron-dense granules numerous. CS highly vascularized.

  1. Microscopia óptica e eletrônica do corpúsculo de Stannius do curimbatá (Prochilodus scrofa Steindachner, 1881) (Osteichthyes, Cypriniformes, Prochilodontidae)

    OpenAIRE

    Elza Ferreira Goldman Pinheiro; Agar Costa Alexandrino; Silvio Ferri

    1986-01-01

    The Stannius corpuscles of Prochilodus scrofa are studied microscopically (both optically and electronically) as well as macroscopically. Two CS, and sometimes 3 or 4, are found per animal. They are pink-coloured organs, rounded in shape and scattered laterally in the first anterior third of the kidney. Each CS is enveloped by a thick connective tissue capsule. The grandular parenchyma consists of only one cellular (PAS-/AF-) type, cylindrical in shape, with oval or rounded nucleus located ec...

  2. Combining morphology and genetics in resolving taxonomy--a systematic revision of spined loaches (Genus Cobitis; Cypriniformes, Actinopterygii in the Adriatic watershed.

    Directory of Open Access Journals (Sweden)

    Ivana Buj

    Full Text Available Taxonomic investigation of spined loaches from Dalmatia and Herzegovina was conducted on specimens from 14 localities. The results of the detailed morphological investigations were combined with genetic data (based on one mitochondrial and two nuclear genes in order to resolve the taxonomic status of each Cobitis population. Among the investigated features of external morphology, the appearance of spots on the caudal fin base turned out to have the greatest diagnostic value. Furthermore, the number of branched fin rays enabled the discrimination of several species. No morphometric character alone could ensure determination of any Cobitis species. Nevertheless, groups of populations that are more similar in their body shapes correspond to mitochondrial phylogenetic lineages. Based on molecular genetic markers, Dalmatian and Herzegovinian spined loaches form independent lineages inside the Adriatic phylogenetic group. Mitochondrial DNA phylogenetic reconstruction revealed six monophyletic lineages, corresponding to six species distributed in the investigated area. The population distributed in Mostarsko blato karstic field in Bosnia and Herzegovina is described as a new species based on a unique combination of morphological characters: a single triangular Canestrini scale; usually 51/2 branched anal fin rays, 61/2 branched dorsal fin rays, 14 branched caudal fin rays; no spots in the surface pigmentation layer on the caudal fin base; scales on the body very small.

  3. Garra arupi, a new cyprinid fish species (Cypriniformes: Cyprinidae) from upper Brahmaputra basin in Arunachal Pradesh, India

    OpenAIRE

    K. Nebeshwar; W. Vishwanath; Das, D.N.

    2009-01-01

    Garra arupi, a new cyprinid fish species is differentiated from its congeners along the base of the Himalaya in Brahmaputra basin by a combination of characters: two pairs of barbels, anterior position of vent (vent to anal distance 52.6-60.0% pelvic to anal distance), a band of 6-9 prominent horny tubercles on the tip of the snout, a submarginal black band on the dorsal fin, and 16 circumpeduncular scales. It is similar to G. kempi in having an anterior position of the vent, and similar to ...

  4. ПЕРВОЕ ОБНАРУЖЕНИЕ ЛЕЩА ABRAMIS BRAMA (CYPRINIFORMES, CYPRINIDAE) В РЕКЕ ЛЕНЕ

    OpenAIRE

    Кириллов, А.; Шахтарин, Д.; Собакина, И.

    2007-01-01

    В р. Лене впервые обнаружен лещ Abramis brama (Linnaeus, 1758). Рассматриваются вопросы расселения леща, приводятся данные по морфологии и росту.

  5. [Differences in the infestation rate of young cyprinid fishes (Cypriniformes) by metacercaria of Posthodiplostomum Cuticola (Digenea, Diplostomatidae) in river and lake systems of the Lake Chany basin (Western Siberia)].

    Science.gov (United States)

    Iadrenkina, E N

    2014-01-01

    A total of 12 fish species were studied for the invasion of P. cuticola (Nordmann, 1832) metacercariae in the Chany Lake estuaries, river and lake systems during different periods of water level. All infected individuals were represented by juveniles of the family Cyprinidae, except for adults of the dace Leuciscus leuciscus. (L.). Under an average water level (2010) the highest rate of fish invasion was revealed in the estuary zone, where the prevalence of infection (PI) constituted 37.5%, 13.4%, and 5.9% for the gudgeon Gobio gobio, the roach Rutilus rutilus (L.), and the dace L. leuciscus (L.), respectively. An infested carp Cyprinus carpio L. (Heckel) (PI - 13.5%) was recorded in the lower Kargat River, and the goldfish Carassius auratus (L.) (PI - 3.2%), in the lake system. Mean intensity of infection (MI) constituted 1.3, 1.4, 2.5, 2.6, and 1.0 in gudgeon, roach, dace, carp, and bream (Abramis brama L.), respectively. During dry season (2011) with high water salinity only two fish species were infected with P. cuticola metacercariae, the goldfish (PI - 7.9%) and the roach (PI - 1.5%). No correlation between PI and fish density was revealed. It is assumed that the high degree of water salinity is a limiting factor regulating the efficiency of cercariae transition from the first intermediate host (Planorbis planorbis (L.)) to the second one (Pisces, Cyprinidae). PMID:25693328

  6. Phylogenetic relationships of Acheilognathidae (Cypriniformes: Cyprinoidea) as revealed from evidence of both nuclear and mitochondrial gene sequence variation: Evidence for necessary taxonomic revision in the family and the identification of cryptic species

    Czech Academy of Sciences Publication Activity Database

    Chang, H.-C.; Li, F.; Shao, K.-T.; Lin, Y.-S.; Morosawa, T.; Kim, S.; Koo, H.; Kim, W.; Lee, J.-S.; He, S.; Smith, Carl; Reichard, Martin; Miya, M.; Sado, T.; Uehara, K.; Lavoué, S.; Chen, W.-J.; Mayden, R. L.

    2014-01-01

    Roč. 81, December (2014), s. 182-194. ISSN 1055-7903 Institutional support: RVO:68081766 Keywords : Acheilognathinae * Cyprinidae * Cryptic species * Nuclear loci * Cytochrome b * European bitterling Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 3.916, year: 2014

  7. Vaade minevikku kaljult mere ääres / Helju Koger

    Index Scriptorium Estoniae

    Koger, Helju, 1943-

    2005-01-01

    Eesti Vabaõhumuuseumis asuvast Kochidele kuulunud Liberty suvemõisast (arhitekt Otto Schott, 1905), puiesteest Via Appia, Arthur Girard de Soucantoni hävinud villast (arhitekt Rudolf Knüpffer), G. de Soucantoni tütre Helene ja Eugen Von Nottbecki suvilast nr. 15, raidkividest, Etienne Girard de Soucantoni šveitsi villast (praegu muuseumi teadurite töömaja) jm. 11 ill

  8. Koomiksivestern / Jaanus Noormets

    Index Scriptorium Estoniae

    Noormets, Jaanus

    2004-01-01

    Prantsuse näitleja Vincent Cassel mängib nimiosa prantsuse koomiksipõhises mängufilmis "Blueberry" : režissöör Jan Kounen. Koomiksi, millest filmi on jõudnud üpris vähe, lõid 1960-ndatel Jean-Michel Charlier ja Jean Girard

  9. Zrimõi sled / Lev Livshits

    Index Scriptorium Estoniae

    Лившиц, Лев, 1924-2013

    2008-01-01

    Tallinna Uue tänava hoonestuse ajaloost. Arhitekt Schotti projekti järgi ehitatud eklektilised hooned Uus t. 3 ja Girard de Soucantoni maja nr.19. Carl Engeli projekti järgi ehitatud elamu Uus t. 16 ja püssirohuait Uus t. 37

  10. Proust: A Collection of Critical Essays. Twentieth Century Views Series.

    Science.gov (United States)

    Girard, Rene, Ed.

    One of a series of works aimed at presenting contemporary critical opinion on major authors, this collection includes essays by Rene Girard, Robert Vigneron, Henri Peyre, Jacques Riviere, Albert Thibaudet, Walter A. Strauss, Germaine Bree, Georges Cattaui, Elliott Coleman, Leo Spitzer, Richard Macksey, Robert Champigny, Charles Du Bos, Ramon…

  11. 放化疗治疗局部进展期胰腺癌的定性系统评价%Chemoradiotherapy in the Management of Locally Advanced Pancreatic Carcinoma: A Qualitative Systematic Review

    Institute of Scientific and Technical Information of China (English)

    李伟雄; 黄凌

    2010-01-01

    @@ 1 文献来源 Huguet F,Girard N,Guerche CS,et al.Chemoradiotherapy in the management of locally advanced pancreatic carcinoma: A qualitative systematic review[J].J Clin Oncol,2009,27(13):2269-2277

  12. An 'Oedipus pattern' in the Old Testament?

    DEFF Research Database (Denmark)

    Jensen, Hans Jørgen Lundager

    2007-01-01

    The Oedipus myth and a scapegoat pattern have been central issues in both Walter Burkert's and René Girard's writings about ancient Greek religion. This article proposes the existence of a comparable pattern in the Old Testament legend about the Ark (1-2 Samuel). Together with other Old Testament...

  13. Making the Gallery Scene

    Science.gov (United States)

    Wasserman, Burton

    1976-01-01

    The furniture, fabrics and interior architectural systems designed by George Nelson, Charles and Ray Eames, Alexander Girard and Robert Propst for Herman Miller, Incorporated, have revolutionized American design by their brilliant use of new materials and exploitation of mass production techniques. A stunning exhibition of their combined efforts…

  14. Graphs of partitions and Ramanujan's tau-function

    OpenAIRE

    Brent, Barry

    2004-01-01

    The invariant z_{lambda} attached to a partition lambda sits in the denominator of the Girard-Waring solution to Newton's symmetric function relations. We interpret Ramanujan's tau-function in terms of z_lambda, and interpret z_lambda in terms of the automorphisms of a graph.

  15. Linear Logic on Petri Nets

    DEFF Research Database (Denmark)

    Engberg, Uffe Henrik; Winskel, Glynn

    This article shows how individual Petri nets form models of Girard's intuitionistic linear logic. It explores questions of expressiveness and completeness of linear logic with respect to this interpretation. An aim is to use Petri nets to give an understanding of linear logic and give some apprai...

  16. "... Urged by His Wife Jezebel"

    DEFF Research Database (Denmark)

    Holt, Else Kragelund

    1995-01-01

    The narrative 1 Kgs 18 of Elijah's battle on M. Carmel with the Baalpriests can be viewed as yet another OT story of the appointment of the scapegoat. Using R. Girard's methdology it appears that the Baalpriests are held responsible for the drougth, and are slaughtered as a kind of sacrifice...

  17. The Sacrificial Crisis

    DEFF Research Database (Denmark)

    Östman, Lars

    2007-01-01

    Med udgangspunkt i Jesus´ lidelseshistorie forsøger artiklen via den franske religionsfilosof René Girards tanker om den hellige krise at knytte an til en generel teori om en tilstand, hvor lov og ret er suspenderede. Gennem en kritisk og perspektiverende læsning af Carl Schmitt forståes denne kr...

  18. Local linear logic for locality consciousness in multiset transformation

    NARCIS (Netherlands)

    H. McEvoy; P.H. Hartel

    1995-01-01

    We use Girard's linear logic (LL) to produce a semantics for Gamma, a multiset transformation language. The semantics improves on the existing structured operational semantics (SOS) of the language by highlighting Gamma's inefficiencies, which were hidden by the SOS. We propose a new logic called lo

  19. Sartre: A Collection of Critical Essays. Twentieth Century Views Series.

    Science.gov (United States)

    Kern, Edith, Ed.

    One of a series of works aimed at presenting contemporary critical opinion on major authors, this collection includes essays by Edith Kern, Claude-Edmonde Magny, Henri Peyre, Kenneth Douglas, Edmund Wilson, Theophil Spoerri, Jacques Guicharnaud, Eric Bentley, Robert Champigny, Oreste F. Pucciani, Frederic Jameson, Rene Girard, Guido…

  20. Microcobitis, a new genus name for Cobitis misgurnoides (Teleostei: Cobitidae)

    Czech Academy of Sciences Publication Activity Database

    Bohlen, Jörg; Harant, R.

    2010-01-01

    Roč. 21, č. 4 (2010), s. 295-300. ISSN 0936-9902 R&D Projects: GA ČR GA206/08/0637; GA ČR GA206/05/2556; GA AV ČR IAA600450508 Institutional research plan: CEZ:AV0Z50450515 Keywords : cypriniformes * fish * guangxi Subject RIV: EG - Zoology Impact factor: 0.828, year: 2010

  1. Formation of oral and pharyngeal dentition in teleosts depends on differential recruitment of retinoic acid signaling

    OpenAIRE

    Gibert, Yann; Bernard, Laure; Debiais-Thibaud, Melanie; Bourrat, Franck; Joly, Jean-Stephane; Pottin, Karen; Meyer, Axel; Retaux, Sylvie; Stock, David W.; Jackman, William R.; Seritrakul, Pawat; Begemann, Gerrit; Laudet, Vincent

    2010-01-01

    One of the goals of evolutionary developmental biology is to link specific adaptations to changes in developmental pathways. The dentition of cypriniform fishes, which in contrast to many other teleost fish species possess pharyngeal teeth but lack oral teeth, provides a suitable model to study the development of feeding adaptations. Here, we have examined the involvement of retinoic acid (RA) in tooth development and show that RA is specifically required to induce the pharyngeal tooth develo...

  2. Babylonian confusion of gudgeons in the west Aegean drainages inferred by the mitochondrial DNA analyses

    OpenAIRE

    Radek Sanda; Jasna Vukic; Michal Nowak; Jan Mendel; Tihomir Stefanov; Stamatis Zogaris

    2015-01-01

    Gudgeons (Gobio and Romanogobio, Cypriniformes) are taxonomically very complicated group of primary freshwater fishes. The region of the west Aegean Sea rivers is inhabited by three species according to Kottelat and Freyhof (2007): Gobio feraensis endemic to the Pinios River basin; Gobio balcanicus inhabiting all remaining drainages, from the Aliakmon up to the Marica drainage; and Romanogobio elimeus occurring from the Pinios to the Vardar basin. Recent analysis of barcoding gene cytochrome ...

  3. Formation of oral and pharyngeal dentition in teleosts depends on differential recruitment of retinoic acid signaling.

    Science.gov (United States)

    Gibert, Yann; Bernard, Laure; Debiais-Thibaud, Melanie; Bourrat, Franck; Joly, Jean-Stephane; Pottin, Karen; Meyer, Axel; Retaux, Sylvie; Stock, David W; Jackman, William R; Seritrakul, Pawat; Begemann, Gerrit; Laudet, Vincent

    2010-09-01

    One of the goals of evolutionary developmental biology is to link specific adaptations to changes in developmental pathways. The dentition of cypriniform fishes, which in contrast to many other teleost fish species possess pharyngeal teeth but lack oral teeth, provides a suitable model to study the development of feeding adaptations. Here, we have examined the involvement of retinoic acid (RA) in tooth development and show that RA is specifically required to induce the pharyngeal tooth developmental program in zebrafish. Perturbation of RA signaling at this stage abolished tooth induction without affecting the development of tooth-associated ceratobranchial bones. We show that this inductive event is dependent on RA synthesis from aldh1a2 in the ventral posterior pharynx. Fibroblast growth factor (FGF) signaling has been shown to be critical for tooth induction in zebrafish, and its loss has been associated with oral tooth loss in cypriniform fishes. Pharmacological treatments targeting the RA and FGF pathways revealed that both pathways act independently during tooth induction. In contrast, we find that in Mexican tetra and medaka, species that also possess oral teeth, both oral and pharyngeal teeth are induced independently of RA. Our analyses suggest an evolutionary scenario in which the gene network controlling tooth development obtained RA dependency in the lineage leading to the cypriniforms. The loss of pharyngeal teeth in this group was cancelled out through a shift in aldh1a2 expression, while oral teeth might have been lost ultimately due to deficient RA signaling in the oral cavity. PMID:20445074

  4. СРАВНИТЕЛЬНАЯ ХАРАКТЕРИСТИКА ПЛОДОВИТОСТИ ABRAMIS BRAMA ( OSTEICHTHYES, CYPRINIFORMES) ДНЕПРОВСКОГО ВОДОХРАНИЛИЩА

    OpenAIRE

    Федоненко, Е.; Косинова, Н.

    2003-01-01

    Зміна темпів росту, статевого дозрівання, вгодованості й плідності риб розглядається як пристосувальна реакція популяції риб до змін умов життя. Вивчення плідності риб дає змогу прогнозувати формування іхтіофауни водосховищ.

  5. The matrix Euler-Fermat theorem

    International Nuclear Information System (INIS)

    We prove many congruences for binomial and multinomial coefficients as well as for the coefficients of the Girard-Newton formula in the theory of symmetric functions. These congruences also imply congruences (modulo powers of primes) for the traces of various powers of matrices with integer elements. We thus have an extension of the matrix Fermat theorem similar to Euler's extension of the numerical little Fermat theorem

  6. A case study of Megaselia scalaris (Diptera: Phoridae) causing ocular myiasis in a western hognose snake.

    Science.gov (United States)

    Diclaro, J W; Lehnert, M S; Mitola, M A; Pereira, R M; Koehler, P G

    2011-07-01

    Late instar larvae of the scuttle fly, Megaselia scalaris Loew, were found near the right eye of a live captive-reared western hognose snake, Heterodon nasicus Baird and Girard. Dissection and removal of the snake's dorsal cranial bones revealed tissue degradation of the infected eye, the optic nerve, and the brain case; we suggest that these factors contributed to the death of this snake. This case study further demonstrates the opportunistic behavior of M. scalaris. PMID:21845957

  7. Interaction Graphs: Exponentials

    OpenAIRE

    Seiller, Thomas

    2013-01-01

    This paper is the fourth of a series exposing a systematic combinatorial approach to Girard's Geometry of Interaction program. This program aims at obtaining particular realizability models for linear logic that accounts for the dynamics of cut-elimination. This fourth paper tackles the complex issue of defining exponential connectives in this framework. In order to succeed in this, we use the notion of graphings, a generalization of graphs which was defined in earlier work. We explain how we...

  8. Sensitivity to chemical stimuli plays a fundamental role in the food preferences. Examples in the evolutionary scale: 1. Role of the walking leg chemoreceptors in the red swamp crayfish Procambarus Clarkii 2. PROP bitter taste sensitivity and its nutritional implications in Humans

    OpenAIRE

    Melis, Melania

    2014-01-01

    In this thesis, we studied two examples of the sensitivity to chemical stimuli and its role in the food preferences in two models of the evolutionary scale. The red swamp crayfish Procambarus clarkii (Girard, 1852) (Crustacea: Decapoda) is an invasive species of freshwater habitats that has spread worldwide. In crayfish, like in other decapod crustaceans, reception of chemical cues occurs by way of peripheral chemoreceptors grouped within sensory hairs and typically located on the cuticle ...

  9. Atividade reprodutiva de Physalaemus signifer (Anura, Leptodactylidae) em ambiente temporário

    OpenAIRE

    Wogel Henrique; Abrunhosa Patrícia A.; Pombal Jr. José P.

    2002-01-01

    The breeding activity of Physalaemus signifer (Girard, 1853) was monitored from July 1999 to July 2000 in a temporary pond in Palmital, Municipality of Saquarema, State of Rio de Janeiro, Atlantic Rain Forest, Brazil. Males were sexually actives only in four nights, arriving at the pond on the early rainy season. Males in amplexus were larger and spent more nights in the reproductive aggregation than solitary ones. The number of nights was correlated with the mass of the males. Males adopted ...

  10. Ecology of Ischnocnema parva (Anura: Brachycephalidae) at the Atlantic Rainforest of Serra da Concórdia, state of Rio de Janeiro, Brazil

    OpenAIRE

    Ana Cristina J. S. Martins; Mara C. Kiefer; Carla C. Siqueira; Monique Van Sluys; Vanderlaine A Menezes; Carlos Frederico D. Rocha

    2010-01-01

    Ischnocnema (Brachycephalidae) includes many species that are important members of the leaf litter frog communities in the Atlantic Rainforest of Brazil. Ischnocnema parva (Girard, 1853) is endemic to the Atlantic Rainforest biome and is restricted to the forests of southeastern Brazil. Currently, the available information about the ecology of I. parva is scarce. We studied the diet, the habitat use, reproduction and density of I. parva in an area of Atlantic Rainforest at the Concórdia mount...

  11. New structures for physics

    Energy Technology Data Exchange (ETDEWEB)

    Coecke, Bob (ed.)

    2011-07-01

    This volume provides a series of tutorials on mathematical structures which recently have gained prominence in physics, ranging from quantum foundations, via quantum information, to quantum gravity. These include the theory of monoidal categories and corresponding graphical calculi, Girard's linear logic, Scott domains, lambda calculus and corresponding logics for typing, topos theory, and more general process structures. Most of these structures are very prominent in computer science; the chapters here are tailored towards an audience of physicists. (orig.)

  12. Optimization of extraction process and investigation of antioxidant effect of polysaccharides from the root of Limonium sinense Kuntze

    OpenAIRE

    Xinhui Tang; Lifang Yan; Jing Gao; Haiyan Ge; Haidong Yang; Na Lin

    2011-01-01

    Objective: To optimize the extraction technology for polysaccharides from the root of Limonium sinense (Girard) Kuntze, Plumbaginaceae and evaluate the antioxidant capacity of polysaccharides from L. sinense (LSEP) Materials and Methods : One-singer factor and response surface methodology(RSM) were established to extract the polysaccharides from L. sinense. Then, the 1,1-diphenyl-2-picrylhydrazyl free radical, hydroxyl radical(.OH), and 2,2′-Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) ...

  13. Knowledge Management at Cory Environmental: A Theoretical and Empirical Analysis

    OpenAIRE

    Carter, Jonathan David

    2007-01-01

    To answer the question "How can Cory make better use of existing knowledge and create the new knowledge necessary to improve company performance?", an analysis of knowledge management practice at Cory Environmental was carried out using the knowledge management enabler model (Girard 2004) as a framework. To keep the project manageable research was restricted to knowledge management in relation to the commercial sales function within Cory, but the assumption was that findings and recommendati...

  14. Evaluation of novel derivatisation reagents for the analysis of oxysterols

    Energy Technology Data Exchange (ETDEWEB)

    Crick, Peter J., E-mail: p.j.crick@swansea.ac.uk [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Aponte, Jennifer; Bentley, T. William [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Matthews, Ian [College of Engineering, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Wang, Yuqin [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Griffiths, William J., E-mail: w.j.griffiths@swansea.ac.uk [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom)

    2014-04-11

    Graphical abstract: - Highlights: • New derivatisation reagents for LC–MS analysis of oxysterols. • New reagents based on Girard P give high ion-currents and informative LC–MS{sup n} spectra. • Permanent charge is vital for efficient MS{sup n} fragmentation. • New reagents offer greater scope for incorporation of isotope labels. - Abstract: Oxysterols are oxidised forms of cholesterol that are intermediates in the synthesis of bile acids and steroid hormones. They are also ligands to nuclear and G protein-coupled receptors. Analysis of oxysterols in biological systems is challenging due to their low abundance coupled with their lack of a strong chromophore and poor ionisation characteristics in mass spectrometry (MS). We have previously used enzyme-assisted derivatisation for sterol analysis (EADSA) to identify and quantitate oxysterols in biological samples. This technique relies on tagging sterols with the Girard P reagent to introduce a charged quaternary ammonium group. Here, we have compared several modified Girard-like reagents and show that the permanent charge is vital for efficient MS{sup n} fragmentation. However, we find that the reagent can be extended to include sites for potential stable isotope labels without a loss of performance.

  15. From a Philosophical point of view, can the Budism Be An Anti-Sacrificial Science?

    Directory of Open Access Journals (Sweden)

    Francisco Felizol Marques

    2014-04-01

    Full Text Available Both on Buddhist tradition and René Girard´s thought ignorance and desire are on the basis of suffering and violence. The Buddhist tradition puts/takes ignorance, that nothing exists in and by itself, avydia as the cause of all sufferings because it generates a chain of desire / aversion that leads us to an imprisonment where we move from desiring an object to another. Girard´s perspective founds violence´s origin on the ignorance of our mimetic desire. The subject ignores that, far from being free, autonomous and differentiated, as the "romantic lie" prays, he only desires and wants by imitating a model. Neither the subject nor the object, which the subject freely thinks to desire, exist free for themselves. While this model prevails, he will react more and more violently to the claims of the subject; and even if the subject overtakes its model, he will greedily seek another and another model, always doomed to deception. If we add to this the proximity of the two anti-sacrificial perspectives, displayed on the descriptive closeness of the samsara´s wheel and the circular and sacrificial time of a pagan society, we find between Girard and the buddhist tradition enough common points for their mutual understanding.

  16. COMPOSIÇÃO DA ICTIOFAUNA DAS BACIAS HIDROGRÁFICAS DO RIO GRANDE DO NORTE, BRASIL

    OpenAIRE

    Wallace Silva do Nascimento; Nirlei Hirachy Costa Barros; Andréa Soares Araújo; Liliane Lima Gurgel; Bhaskara Canan; Wagner Franco Molina; Ricardo S. Rosa; Sathyabama Chellappa

    2014-01-01

    Aqui é apresentado um extenso levantamento atualizado da composição das espécies de peixes de água doce das bacias hidrográficas do Estado do Rio Grande do Norte. A compilação foi realizada a partir de levantamentos préviose dados da literatura sobre o registro das espécies, reportadas no período de 1982 a 2013. Foram identificadas 51 espécies, distribuídas em dezordens (Cypriniformes, Characiformes, Perciformes, Siluriformes, Synbranchiformes, Cyprinodontiformes, Gymnotiformes, Gasterosteifo...

  17. Trichodina modesta: an exotic ciliate in the Neotropical region parasitizing an unusual host.

    Science.gov (United States)

    Valladão, Gustavo Moraes Ramos; Giannecchini, Luiz Gustavo; Martins, Maurício Laterça; de Pádua, Santiago Benites

    2015-01-01

    In this study, an important ornamental fish, Betta splendens (Osphronemidae), from three different Brazilian states was examined for parasitic infestations. Smears with parasites were impregnated with silver nitrate or stained using Giemsa for taxonomic evaluation. A disc-shaped trichodinid with a body diameter of 39.7 ± 3.3 µm, adhesive disc diameter of 32.9 ± 3.1 µm and denticulate ring diameter of 19.5 ± 2.0 µm was found. The morphological characteristics resembled those of Trichodina modesta Lom, 1970, a species that shows clear host specificity for Cypriniformes. Until now, its occurrence was restricted to the Eurasian region. In the present study, a new host for T. modesta is reported and therefore the first occurrence of this species in the Americas. The parasite was possibly introduced into the Neotropical region through the exotic fish trade, especially of Cypriniformes used by aquarists. The distribution of this ciliate is discussed and a checklist of localities and hosts for the species is provided. PMID:26154956

  18. Diet composition of post-metamorphic bullfrogs (Rana catesbeiana) in the Zhoushan archipelago,Zhejiang Province, China

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Bullfrogs (Runa catesbeiana) are listed as one of the 100 worst invasive alien species in the world.They are generalist predators and thus may affect native species through predation.In previous studies,the food contents of bullfrogs were mostly examined at a single site.In the present study,the diet composition of post-metamorphic bullfrogs on eight islands (Daishan,Liuheng,Xiushan,Fodu,Taohua,Xiashi,Cezi,and Putuoshan) in the Zhoushan Archipelago,Zhejiang Province of China,were examined by using the stomach flushing method from June 30 to August 11 in 2005.A total 0f391 individual frogs were measured,including 113 adults and 278 juveniles.The analysis of the stomach contents shows that,for adult bullfrogs,the most important prey (by diet volume) overall were Decapoda,Coleoptera,Odonata,Mesogastropoda,Raniformes,and Cypriniformes.For juvenile bullfrogs,these were Decapoda,Coleoptera,Cypriniformes,Odonata,Orthoptera,Hymenoptera,Lepidoptera larvae,Mesogastropoda,and Raniformes.Moreover,the prey size and diet volume increased with the body size of both adult and juvenile bullfrogs.The diet composition of primary preys of bullfrogs was significantly different among the islands.The results indicate that bullfrogs exert different predatory influences on native fauna at different sites and that bullfrogs are generalist predators with extensive ecological impacts on native fauna.

  19. Development of the splanchnocranium in Prochilodus argenteus (Teleostei: Characiformes) with a discussion of the basal developmental patterns in the Otophysi.

    Science.gov (United States)

    Carvalho, Murilo; Vari, Richard P

    2015-02-01

    Development of the mandibular, hyoid and gill arches, which constitute the splanchnocranium, are described for Prochilodus argenteus, order Characiformes, one of the basal lineages of the Otophysi. Development was examined from just hatched larvae through juveniles using whole specimens cleared and counterstained for cartilage and bone as well as histological preparations. Observations are compared with the developmental trends reported for Cypriniformes, the basalmost clade of the Otophysi. Shortened developmental sequences for Prochilodus compared to the cypriniform Catostomus were discovered in the ontogeny of the ceratohyals, ceratobranchials 1-5, epibranchials 1-4 and the symplectic portion of the hyosymplectic. Prochilodus also differs from Catostomus in having the basihyal plus the anterior copula appearing at different stages of ontogeny rather than simultaneously. Contrary to previous assumptions, developmental information indicates that hypobranchial 4 as well as likely basibranchial 5 are present in Prochilodus. Various developmental patterns in Prochilodus considered basal for the Otophysi, the predominant component of the Ostariophysi, are likely conserved from patterns prevalent in basal groups in the Actinopterygii. PMID:25595854

  20. Testemunho, desconstrução e informação : pensando nas margens de uma interpretação sacrificial da cultura

    OpenAIRE

    Catalão, Helena Barroso

    2014-01-01

    Neste trabalho de investigação, procuramos fundamentar a tese de que o testemunho (absoluto) é o princípio indesconstrutível da desconstrução da sociedade da informação, desconstrução da qual emana a figura exemplar do «jornalista testemunhal». Esta tese não poderia ser sustentada sem uma outra ideia que forjamos na confluência dos trabalhos de J. Derrida, P. Ricoeur e R. Girard: o testemunho (absoluto) é a matriz da cultura ocidental, englobando e excedendo uma interpretação s...

  1. Evaluating the toxicity of oil of lemon eucalyptus, Corymbia citriodora (Hook.), against larvae of the Asian tiger mosquito and non-target fish and larval amphibians

    OpenAIRE

    Escartin, Santi; Mariani, Simone

    2014-01-01

    Hemos probado la toxicidad del aceite del eucalipto limón (OLE) contra las larvas de mosquito tigre Aedes (Stegomya) albopictus (Skuse, 1895) (Diptera: Culicidae) y contra vertebrados acuáticos no objetivo, el pez Gambusia affinis (Baird and Girard, 1853) y el renacuajo de la rana Pelophylax perezi (López-Seodane, 1885). La mezcla acuosa de OLE fue efectiva como larvicida y letal para gambúsias y renacuajos. La mezcla acuosa expuesta durante una semana al aire libre no tu...

  2. A taxonomic revision of the Phrynosoma douglasii species complex (Squamata: Phrynosomatidae).

    Science.gov (United States)

    Montanucci, Richard R

    2015-01-01

    Short-horned lizards (Phrynosoma douglasii species complex) occur throughout the inter-montane West and Great Plains of western North America. The comparative morphology and color pattern variation of short-horned lizards was studied in 3,174 specimens. Multivariate analyses of 20 morphological and color-pattern characters were applied to 977 specimens, and univariate statistics were summarized for 52 samples totaling 1,134 specimens. The results of the morphological data analyses support the recognition of P. douglasii (Bell 1828) as a distinct species, and the resurrection of P. brevirostris Girard 1858a and P. ornatissimum Girard 1858a as species distinct from P. hernandesi Girard 1858a. Two new species allied to P. brevirostris are described: P. bauri sp. nov. from the eastern plains of Colorado and northeastern New Mexico, southeastern Wyoming and southwestern Nebraska south of the North Platte River, and P. diminutum sp. nov. endemic to the San Luis Valley of southern Colorado and northern New Mexico. The Mexican taxon brachycercum Smith 1942 is reassigned as a subspecies of P. ornatissimum, based on non-discrete character differences and overall morphometric similarity. The ranges of P. hernandesi and P. ornatissimum broadly overlap in central New Mexico, the former taxon occupying the coniferous forests of disjunct mountain ranges, the latter occuring in the surrounding desert grasslands. Principal components analysis has revealed morphological evidence of hybridization where the two taxa meet, generally within ecotones between montane forest associations and grasslands. Principal components analysis has also revealed a high level of morphological variability in populations occurring in the Colorado Plateau region of northeastern Arizona, northwestern New Mexico, extreme southwestern Colorado and adjacent Utah. The evidence suggests that these populations arose through past hybridization between the two species. The taxon ornatum Girard 1858b, although

  3. IMITATION AGAINST ORIGINAL: CİNGÖZ RECAİ VERSUS SHERLOCK HOLMES
    TAKLİT ORİJİNALE KARŞI: CİNGÖZ RECAİ VERSUS SHERLOCK HOLMES

    OpenAIRE

    Seval ŞAHİN

    2011-01-01

    In this article is carried out Cingöz Recai against Sherlock Holmes, which is published in 1928 by Peyami Safa using penname Server Bedi by way of taking into consideration Homi Bhabba, René Girard, Fredric Jameson and Nurdan Gürbilek’s aproach on imitation and original. Cingöz Recai is brought a different approach on the relationship between imitation and original by strategy created by the author’s explanation about both the conflict of East-West and the imitation and original. Bu makaled...

  4. Call-by-name, call-by-value, call-by-need and the linear lambda calculus

    OpenAIRE

    Maraist, J.; Odersky, M.; Turner, D.N.; Wadler, P.

    1999-01-01

    Girard described two translations of intuitionistic logic into linear logic, one where A→B maps to (!A⊸B) and another where it maps to !(A⊸B). We detail the action of these translations on terms and show that the first corresponds to a call-by-name calculus, while the second corresponds to call-by-value. We further show that if the target of the translation is taken to be an affine calculus, where! controls contraction but weakening is allowed everywhere, then the second translation correspon...

  5. Call-by-name, Call-by-value, Call-by-need, and the Linear Lambda Calculus

    OpenAIRE

    Maraist, John; Odersky, Martin; Turner, David N.; Wadler, Philip

    1995-01-01

    Girard described two translations of intuitionistic logic into linear logic, one where A -> B maps to (!A) -o B, and another where it maps to !(A -o B). We detail the action of these translations on terms, and show that the first corresponds to a call-by-name calculus, while the second corresponds to call-by-value. We further show that if the target of the translation is taken to be an affine calculus, where ! controls contraction but weakening is allowed everywhere, then the second transl...

  6. " 'Achever Clausewitz' ? -Catastrophisme et apocalypse contemporains"

    OpenAIRE

    Ramond, Charles

    2010-01-01

    Ce texte propose une confrontation entre la doctrine apocalyptique de René Girard (telle qu'elle s'exprime principalement dans son ouvrage 'Achever Clausewitz' de 2007) et celle de Günther Anders (notamment à partir de son livre 'Hiroshima est partout', 1995, tr. fr. 2008), sous l'angle de leur économie conceptuelle et de leur cohérence. Il met en évidence, pour finir, les paradoxes performatifs du discours apocalyptique, qui ne peut fonder son succès que sur ses échecs.

  7. Improvement of cobalt-transport in axons by complexing agents.

    Science.gov (United States)

    Gallyas, F; Lénárd, L; Lázár, G

    1978-09-01

    The use of the cobalt technique is limited by the fact that cobaltous ions travel within axons for a shorter distance than do other intracellular markers. In the present experiments different organic cobaltous complexes were tested in the rat's sciatic nerve. Cobaltous complexes containing ornithine, threonine, lysine or Girard's reagent travelled two or three times further than did the cobaltous ions alone. Using the lysine complex in the frog's visual system, very fine terminals were observed which have never been demonstrated with other techniques. The possible use of other metal complexes as intracellular markers are also discussed. PMID:19605220

  8. Palau-del-Vidre

    OpenAIRE

    Catafau, Aymat

    2014-01-01

    Securinianum sive Palatium Radegarium, 899, appelé ensuite Palatium, église Sainte-Marie, 1100. Le toponyme évoque une résidence aristocratique gallo-romaine qui correspond peut-être aux restes d'une luxueuse villa retrouvée non loin du village. Il aurait pu s'agir d'une demeure sénatoriale, au pied des murailles d'Elne, devenue au Bas-Empire la véritable capitale du Roussillon. Regroupement villageois : Le comte Gaufred et Girard son fils donnent au Temple, en 1155, un manse, une ferragine e...

  9. Apocalyptic Narcissism and the Difficulty of Mourning

    Directory of Open Access Journals (Sweden)

    Florian Mussgnug

    2015-11-01

    Full Text Available In this article I examine how death and loss feature in recent apocalypse fiction and suggest that, in a genre mostly concerned with finitude, there appears to be paradoxically little room for expressions of mourning. I assess contemporary attitudes towards mortality through the writings of Philippe Ariès, Zygmunt Bauman, Simon Critchley and others, and propose a psychoanalytic reading of solitary survivor narrative, inspired by the work of Martin Jay. In the final part of the article, I turn to Sigmund Freud and René Girard to explore the relation between apocalyptic teleology, melancholy, and the expectation of global catastrophe.

  10. Trends in the history of research on the problem of violence in the Old Testament

    Directory of Open Access Journals (Sweden)

    S. D. Snyman

    1997-08-01

    Full Text Available Violence as theological problem is a relative newcomer to the scene of Old Testament studies. It was only during the 1970�s that violence was given major attention by Old Testament scholars. In a number of studies the main focus was on Yahweh and his relation to violence. By the late I970�s the theories of Rene Girard on violence were applied to the Old Testament and played an important role in the thinking of Old Testament scholars on violence. In the last part of the article proposed solutions to the problem of violence in the Old Testament are discussed.

  11. Geometry of Interaction for MALL via Hughes-vanGlabbeek Proof-Nets

    OpenAIRE

    Hamano, Masahiro

    2015-01-01

    This paper presents, for the first time, a Geometry of Interaction (GoI) interpretation using Hughes-vanGlabbeek (HvG) proof-nets for multiplicative additive linear logic (MALL). Our GoI captures dynamically HvG's geometric correctness criterion--the toggling cycle condition--in terms of algebraic operators. Our new ingredient is a scalar extension of the *-algebra in Girard's *-ring of partial isometries over a boolean polynomial ring with literals of eigenweights as indeterminates. In order...

  12. Lack of myristoylation of poliovirus capsid polypeptide VP0 prevents the formation of virions or results in the assembly of noninfectious virus particles.

    OpenAIRE

    Marc, D.; Masson, G.; Girard, M.; Van Der Werf, S.

    1990-01-01

    We previously described the generation of a set of mutations into a cDNA of poliovirus type 1 in the myristoylation signal of the capsid polypeptide VP4 (D. Marc, G. Drugeon, A.-L. Haenni, M. Girard, and S. van der Werf, EMBO J. 8:2661, 1989). Genomic transcripts synthesized in vitro from the mutated cDNAs were found to be noninfectious upon transfection of permissive cells, and this property correlated with the lack of VP0 myristoylation in vivo. In the study presented here, we analyzed the ...

  13. Consideraciones generales acerca de la lógica lineal

    OpenAIRE

    Nunes de Medeiros, Maria da Paz; Universidade Federal de Rio Grande do Norte

    2013-01-01

    En este trabajo se hacen consideraciones generales acerca de la lógica lineal, tomando especialmente en cuenta sus motivaciones y su formalización. En primer lugar, se explica la necesidad de expandir el conjunto usual de conectivas lógicas en la lógica lineal. Siguiendo a Girard (1987), se presenta una formalización para la lógica lineal como un cálculo de secuentes y se esboza el teorema de eliminación de corte para este sistema. Finalmente, se lleva a cabo un análisis crítico de la traducc...

  14. Sin chivo expiatorio, no hay grupo: el caso de las administraciones públicas (No scapegoat, no group: the case of public administrations

    Directory of Open Access Journals (Sweden)

    Josu Bingen Fernández Alcalde

    2012-07-01

    Full Text Available Con base en Girard, Bion, Tuckman, Morgan, Anzieu, o la Sociología Clínica se analiza la posición de “Chivo Expiatorio” como suceso universal en los procesos de desarrollo de grupos y equipos. Una etapa esperable y gestionable de su evolución; no un accidente a evitar ni un motivo para desmantelar los equipos laborales puestos en marcha. Sin chivo expiatorio no hay grupo, ni regeneración grupal. Toda grupalidad vive cíclicamente “el destronamiento” de una figura anterior de liderazgo con algún tipo de violencia sacrificial. Tal acontecimiento cierra una fase de conflicto importante e inaugura una renormalización grupal. Por ello, propone incluirla como fase propia en los modelos generales de desarrollo grupal.ABSTRACTBased on Girard, Bion, Tuckman, Morgan, Anzieu, or Clinical Sociology analyzes the position of "Scapegoat" as a universal event in the development processes of groups and work groups. An expected and manageable stage of its evolution, not an accident to avoid nor a reason for dismantling the set up work teams. No scapegoat no group, no regeneration group. All groups lives cyclically "the dethronement" of an earlier figure of leadership with some sort of sacrificial violence. This event closes a major conflict phase and inaugurates a renormalization group. It is therefore proposed to include itself as a phase in the generic models of group development.

  15. L'orizzonte contemporaneo della fine del mondo. Lineamenti per una dialettica apocalisse/sopravvivenza

    Directory of Open Access Journals (Sweden)

    Delio Salottolo

    2012-01-01

    Full Text Available The aim of this article is to show how important is the idea of “apocalypse” in the present age and discuss if it is possible to develop any strategies of “survival”. After discussing some elements of de Martino’s thought about the temporal, psychopathological and political meaning of “apocalypse”, we’ll analyze the sense of Lars von Trier’s Melancholia as a symptom of the contemporary situation. After that, we’ll point out the importance of 11/9, the biggest and most shocking event of our time, debating the opinions of Baudrillard and Girard. Both thinkers consider 11/9 a fundamental event in order to understand the meaning of our time, Baudrillard debating the problem of our virtual society and Girard talking about the signs of a next “apocalypse”. Finally, we’ll try to introduce the concept of a “politics of survival”, such as it has been recently discussed by Didi-Huberman in his important book, Survivance des lucioles.

  16. COLOMBIA: ¿UN PAÍS DÓNDE SE MUERE EN VANO?: Una interpretación antropológica de la vida y obra del humorista Jaime Garzón (1960-1999 COLOMBIA: A COUNTRY WHERE PEOPLE DIE IN VAIN?: An anthropological interpretation of the life and works of the comedian Jaime Garzón (1960-1999

    Directory of Open Access Journals (Sweden)

    Jorge Salazar Isaza

    2011-08-01

    Full Text Available A la luz de la teoría mimética de René Girard la vida y obra del humorista Jaime Garzón se presenta como una acción profética que devela las causas de la violencia en Colombia. Celebrar la memoria de este artista se erige en un deber ciudadano: un instrumento que contribuye a formar una opinión pública ilustrada y un pensamiento por la paz enraizado en la cultura del país. Este artículo es un aporte sobre la religión en términos antropológicos y abre nuevas perspectivas para considerar la fe cristiana como una dimensión trascendente que se manifiesta en el camino de la justicia social.Under the light of the mimetic theory of René Girard, the life and works of the comedian Jaime Garzón is shown as a prophetic motion revealing the causes of violence in Colombia. Celebrating this artist.s memory stands a civic duty: an instrument that contributes to building an educated public opinion and a thought for peace, rooted in the country.s culture. This article is a contribution on religion in anthropological terms and opens new perspectives to regard the Christian faith as a transcendent dimension that manifests itself on the pathway to social justice.

  17. Precise and accurate assay of pregnenolone and five other neurosteroids in monkey brain tissue by LC-MS/MS.

    Science.gov (United States)

    Dury, Alain Y; Ke, Yuyong; Labrie, Fernand

    2016-09-01

    A series of steroids present in the brain have been named "neurosteroids" following the possibility of their role in the central nervous system impairments such as anxiety disorders, depression, premenstrual dysphoric disorder (PMDD), addiction, or even neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Study of their potential role requires a sensitive and accurate assay of their concentration in the monkey brain, the closest model to the human. We have thus developed a robust, precise and accurate liquid chromatography-tandem mass spectrometry method for the assay of pregnenolone, pregnanolone, epipregnanolone, allopregnanolone, epiallopregnanolone, and androsterone in the cynomolgus monkey brain. The extraction method includes a thorough sample cleanup using protein precipitation and phospholipid removal, followed by hexane liquid-liquid extraction and a Girard T ketone-specific derivatization. This method opens the possibility of investigating the potential implication of these six steroids in the most suitable animal model for neurosteroid-related research. PMID:27378657

  18. Considerazioni mimetiche su Il perturbante (Das Unheimliche

    Directory of Open Access Journals (Sweden)

    Emanuele Antonelli

    2010-12-01

    Full Text Available Si affronta una revisione della categoria psicanalitica del perturbante, con attenzione alla genesi della nozione nell’opera di Freud. Si propone quindi un’ipotesi di lettura, mediante applicazione del paradigma offerto dalla teoria mimetica di René Girard, al fine di rivedere le fondamenta antropologiche dell’esperienza codificata nel testo freudiano. Attraverso un’analisi etimologica del lemma italiano, si discute l’opportunità delle critiche rivolte alla traduzione originale, mettendo anzi in luce la complessità semantica di tale soluzione e le potenzialità esplicative che essa aggiunge alla già ampia, e nota, estensione del lemma tedesco. Con riferimento alla filosofia della storia di matrice girardiana, si mette a fuoco il ruolo della temporalità sia dal punto di vista della genesi della determinazione analitica, sia dal punto di vista delle condizioni di possibilità storiche dell’esperienza in esame.

  19. Introduction

    OpenAIRE

    Bennassar, Bartolomé

    2013-01-01

    Pourquoi Valladolid ? Tous les historiens contemporains sont avertis de l’importance de l’histoire de la péninsule ibérique au xvie siècle comme clé de l’histoire européenne des temps modernes. Préparée par les travaux d’Albert Girard, confirmée par ceux d’Earl J. Hamilton, la découverte de cette importance fut consacrée par La Méditerranée de Fernand Braudel : de vieux problèmes étaient résolus mais des hypothèses neuves étaient formulées, des voies inconnues ouvertes, qui appelaient d’autre...

  20. Whisky

    Directory of Open Access Journals (Sweden)

    Boris Jeanne

    2005-02-01

    Full Text Available Le clown blanc ne sourit jamais, pourtant il est drôle. On force les protagonistes de Whisky à sourire, mais on n’est jamais sûr d’avoir vraiment le droit de rire devant cette histoire simple de vies bien réglées, dérangées par un intrus, à mi-chemin entre Théorème (pour l’intrus et René Girard (pour le triangle amoureux transportés à Carrasco, petite ville d’Uruguay. Tout cela filmé façon Kaurismaki. Un cinquantenaire bourru y fabrique des chaussettes avec deux employées, et Marta son con...

  1. El eterno marido que no sabía amar: Un parásito del deseo en la novelística de Dostoievski

    OpenAIRE

    Rivera León, Lorena

    2015-01-01

    Tomando como punto de partida los análisis de Denis de Rougemont en torno al concepto de amor-pasión en “El amor y Occidente”, así como la caracterización del triángulo del deseo mimético que René Girard realiza en “Mentira romántica y verdad novelesca”, examinamos la presencia del adulterio en dos textos que, pese a su distancia temporal, están íntimamente emparentados. El primero de ellos es “El curioso impertinente”, que encontramos intercalado dentro del “Quijote” como pequeña historia ap...

  2. Atividade reprodutiva de Physalaemus signifer (Anura, Leptodactylidae em ambiente temporário

    Directory of Open Access Journals (Sweden)

    Wogel Henrique

    2002-01-01

    Full Text Available The breeding activity of Physalaemus signifer (Girard, 1853 was monitored from July 1999 to July 2000 in a temporary pond in Palmital, Municipality of Saquarema, State of Rio de Janeiro, Atlantic Rain Forest, Brazil. Males were sexually actives only in four nights, arriving at the pond on the early rainy season. Males in amplexus were larger and spent more nights in the reproductive aggregation than solitary ones. The number of nights was correlated with the mass of the males. Males adopted satellite behavior as alternative tactic for mate acquisition. Changes between calling and satellite tactics were observed in different nights. Calling males were not larger and heavier than satellite ones. Satellite behavior seemed to be related with the order of arrival on the pond. Size and mass of males did not influence the results of fights. Resident males won the majority of agonistics combats. Three types of vocalizations are described: advertisement, territorial, and encounter calls.

  3. Energy and environment

    International Nuclear Information System (INIS)

    This special dossier 'energy and environment' is made of 10 contributions dealing with: Man and energy, terrible lovers (Jean-Marc Jancovici); Wood fuel for southern countries (Jacques Valeix, Alain Bertrand, Philippe Girard); Energy and water: study of large-scale interactions, some medium-and long-term impacts (Jean-Francois Bonnet, Alain Ayong-Le-Kama, Stephane Cai); What solution for a sustainable nuclear energy? (Elisabeth Huffer); Opening of electricity markets: why? How? What environmental impacts? (Stephane Mattatia); Electricity and renewable energies: how far is the national grid capable to manage decentralized power sources? (Andre Merlin); Hydrogen, tomorrow's energy vector? (Jerome Perrin, Jean-Francois Deschamps; Energy in developing countries: the International Energy Agency (IEA) scenario (Cedric Philibert, Francois Cattier); The energy R and D program in the USA (Guillaume de Smedt); Energy mastery, the energy priority of the century (Pierre Radanne)

  4. Ludics and Web: Another Reading of Standard Operations

    Science.gov (United States)

    Fouqueré, Christophe

    The development of the Web lead to new programming languages. They merely come from well-known sequential languages augmented by specific libraries dedicated to web usage. They do not seriously take into account interaction, that is the most important principle in action. Relevant to the dialogue paradigm, we show that a web language may be fully designed in this spirit. We explain in which extent interaction is a central concept in web analysis. For that purpose, we use ludics as a logical framework. Ludics was developed by J.-Y. Girard as a semantics able to rebuild the logics from the notion of interaction. We present then a concrete web language whose type system is derived from ludics.

  5. MONOGENEA OF EXOTIC FISHES I.DESCRIPTION OF Salsuginus bermudae FROM Gambusia affinis FOR THE FIRST TIME IN CHINA%外来鱼类单殖吸虫研究Ⅰ.食蚊鱼寄生百慕大伴盐虫Salsuginus bermudae首次在我国检出

    Institute of Scientific and Technical Information of China (English)

    熊向英; 丁雪娟

    2011-01-01

    This paper reports a newly recorded monogenean in China, Salsuginus bermudae Rand & Wiles, 1987, found on the gills of an exotic fish, Gambusia affinis Baird and Girard, from Guangzhou, Guangdong Province. The shape of our specimens basically agrees with the original description except for the dimensions of the whole worms, the shape of the connective bars and the number of the marginal hooks.%记录采自广东广州食蚊鱼Gambusia-affinis鳃上的1种单殖吸虫——百慕大伴盐虫Salsuginus bermudae Rand & Wiles,1987.所获标本与Rand & Wiles的原始描述基本一致,但在量度、边缘小钩数量、联结片的结构上略有差异.伴盐虫属及百慕大伴盐虫均为我国首次报道.

  6. IMITATION AGAINST ORIGINAL: CİNGÖZ RECAİ VERSUS SHERLOCK HOLMES TAKLİT ORİJİNALE KARŞI: CİNGÖZ RECAİ VERSUS SHERLOCK HOLMES

    Directory of Open Access Journals (Sweden)

    Seval ŞAHİN

    2011-01-01

    Full Text Available In this article is carried out Cingöz Recai against Sherlock Holmes, which is published in 1928 by Peyami Safa using penname Server Bedi by way of taking into consideration Homi Bhabba, René Girard, Fredric Jameson and Nurdan Gürbilek’s aproach on imitation and original. Cingöz Recai is brought a different approach on the relationship between imitation and original by strategy created by the author’s explanation about both the conflict of East-West and the imitation and original. Bu makalede Peyami Safa’nın Server Bedi takma adıyla yazdığı ve 1928 yılında yayımlanan Sherlock Holmes’e Karşı Cingöz Recai serileri üzerinde durulmuştur. İncelemede Homi Bhabba, René Girard, Fredric Jameson ve Nurdan Gürbilek’in taklit ve orijinal ilişkisine dair yaklaşımlarından faydalanılmıştır. Cingöz Recai, Türk edebiyatında taklit ve orijinal ilişkisine farklı bir yaklaşım getirmiştir. Bu yaklaşımda yazarın kahramanı üzerinden ortaya koyduğu strateji bir taraftan Doğu-Batı meselesini gündeme getirirken diğer taraftan taklit ve orijinal ilişkisini sorgulamaya açar.

  7. La idealización del amor y la mujer en La vorágine The idealization of love and woman in The Vortex

    Directory of Open Access Journals (Sweden)

    Carlos Daniel Ortiz Caraballo

    2008-12-01

    Full Text Available El trabajo a realizar es un análisis sociocrítico de La vorágine (1924, de José Eustasio Rivera (Neiva, 1888-Nueva York, 1928. Su objetivo es dilucidar una de las tomas de posición presente en esta obra literaria, específicamente, la toma de posición romántica en relación con sus personajes femeninos y los valores socio-económicos de la modernidad y el capitalismo, para establecer su impacto en el campo literario colombiano. El análisis textual se encuentra ordenado a partir de los conceptos de posición romántica (Girard, 1963, cronotopo (Bajtín, 1986: 269 héroe novelesco (Lukács, 1974: 161-162 y sistema de personajes como ejes fundamentales en los que se despliega buena parte de la axiología de la novelaThis essay is a sociocritic analysis of the novel La vorágine (1924, written by José Eustasio Rivera (Neiva, 1888-New York, 1928. Its objective is to elucidate one of the existent axiological position presented in this literary work, specifically, the romantic one (Girard, 1963. The reading of the text is organized in two parts: first, the idealization of love, where it is established the construction of the romantic position taken in terms of love, the socioeconomic values of modernity and capitalism, taking into account the hero of the novel and the idealization of a woman, according to the women characters and their ideological voice.

  8. Studies on the analysis of 25-hydroxyvitamin D{sub 3} by isotope-dilution liquid chromatography–tandem mass spectrometry using enzyme-assisted derivatisation

    Energy Technology Data Exchange (ETDEWEB)

    Abdel-Khalik, Jonas, E-mail: J.A.F.A.ABDEL-KHALIK.744116@swansea.ac.uk [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Crick, Peter J. [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Carter, Graham D. [DEQAS, Imperial College Healthcare NHS Trust, Clinical Biochemistry Department, Charing Cross Hospital, Fulham Palace Rd, London W6 8RF (United Kingdom); Makin, Hugh L. [Barts and the Royal London School of Medicine and Dentistry, Queen Mary University of London, London E1 2AD (United Kingdom); Wang, Yuqin [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom); Griffiths, William J., E-mail: w.j.griffiths@swansea.ac.uk [Institute of Mass Spectrometry, College of Medicine, Swansea University, Singleton Park, Swansea SA2 8PP (United Kingdom)

    2014-04-11

    Highlights: • New method for the analysis of 25-hydroxyvitamin D{sub 3} exploiting Girard P derivatisation. • Method also applicable to vitamin D{sub 3}, 1α,25- and 24,25-dihydroxyvitamin D{sub 3}. • By modification of the method 3-epi-25-hydroxyvitamin D{sub 3} can also be analysed. - Abstract: The total serum concentration of 25-hydroxyvitamins D (25-hydroxyvitamin D{sub 3} and 25-hydroxyvitamin D{sub 2}) is currently used as an indicator of vitamins D status. Vitamins D insufficiency is claimed to be associated with multiple diseases, thus accurate and precise reference methods for the quantification of 25-hydroxyvitamins D are needed. Here we present a novel enzyme-assisted derivatisation method for the analysis of vitamins D metabolites in adult serum utilising 25-[26,26,26,27,27,27-{sup 2}H{sub 6}]hydroxyvitamin D{sub 3} as the internal standard. Extraction of 25-hydroxyvitamins D from serum is performed with acetonitrile, which is shown to be more efficient than ethanol. Cholesterol oxidase is used to oxidize the 3β-hydroxy group in the vitamins D metabolites followed by derivatisation of the newly formed 3-oxo group with Girard P reagent. 17β-Hydroxysteroid dehydrogenase type 10 is shown to oxidize selectively the 3α-hydroxy group in the 3α-hydroxy epimer of 25-hydroxyvitamin D{sub 3}. Quantification is achieved by isotope-dilution liquid chromatography–tandem mass spectrometry. Recovery experiments for 25-hydroxyvitamin D{sub 3} performed on adult human serum give recovery of 102–106%. Furthermore in addition to 25-hydroxyvitamin D{sub 3}, 24,25-dihydroxyvitamin D{sub 3} and other uncharacterised dihydroxy metabolites, were detected in adult human serum.

  9. Studies on the analysis of 25-hydroxyvitamin D3 by isotope-dilution liquid chromatography–tandem mass spectrometry using enzyme-assisted derivatisation

    International Nuclear Information System (INIS)

    Highlights: • New method for the analysis of 25-hydroxyvitamin D3 exploiting Girard P derivatisation. • Method also applicable to vitamin D3, 1α,25- and 24,25-dihydroxyvitamin D3. • By modification of the method 3-epi-25-hydroxyvitamin D3 can also be analysed. - Abstract: The total serum concentration of 25-hydroxyvitamins D (25-hydroxyvitamin D3 and 25-hydroxyvitamin D2) is currently used as an indicator of vitamins D status. Vitamins D insufficiency is claimed to be associated with multiple diseases, thus accurate and precise reference methods for the quantification of 25-hydroxyvitamins D are needed. Here we present a novel enzyme-assisted derivatisation method for the analysis of vitamins D metabolites in adult serum utilising 25-[26,26,26,27,27,27-2H6]hydroxyvitamin D3 as the internal standard. Extraction of 25-hydroxyvitamins D from serum is performed with acetonitrile, which is shown to be more efficient than ethanol. Cholesterol oxidase is used to oxidize the 3β-hydroxy group in the vitamins D metabolites followed by derivatisation of the newly formed 3-oxo group with Girard P reagent. 17β-Hydroxysteroid dehydrogenase type 10 is shown to oxidize selectively the 3α-hydroxy group in the 3α-hydroxy epimer of 25-hydroxyvitamin D3. Quantification is achieved by isotope-dilution liquid chromatography–tandem mass spectrometry. Recovery experiments for 25-hydroxyvitamin D3 performed on adult human serum give recovery of 102–106%. Furthermore in addition to 25-hydroxyvitamin D3, 24,25-dihydroxyvitamin D3 and other uncharacterised dihydroxy metabolites, were detected in adult human serum

  10. Mitochondrial genome of the shorthead catfish (Pelteobagrus eupogon): structure, phylogeny, and intraspecific variation.

    Science.gov (United States)

    Wang, R-Q; Wang, D-Z; Li, C-T; Yang, X-R

    2016-01-01

    The complete 16,532-nucleotide sequence of the mitochondrial genome of the shorthead catfish (Pelteobagrus eupogon) was determined using the long and accurate polymerase chain reaction method, and compared with the mitochondrial genome sequences of 49 other catfish species belonging to the order Siluriformes. The locations of protein-coding genes and ribosomal ribonucleic acids (RNAs) were identified by comparison with known sequences of other catfishes, including P. fulvidraco and P. nitidus. The P. eupogon mitochondrial genome was composed of 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNA genes, and a non-coding control region. The gene order was identical to that of other Siluriformes. Phylogenetic analyses based on mitochondrial 12S ribosomal RNA, 16S ribosomal RNA, and 13 protein-coding gene sequence data sets were carried out to further clarify the relative phylogenetic position of P. eupogon, and identify phylogenetic relationships among 24 families of Siluriformes. Phylogenetic analyses Randomized Axelerated Maximum Likelihood (RAxML) 8.0.X were congruent with a basal split of the order into Clupeiformes, Characiformes, Cypriniformes, and Siluriformes, and supported a closer relationship of P. eupogon with Amblycipitidae than Siluridae. We therefore concluded that this species appears to be closely related to the Amblycipitidae. In the phylogenetic tree, the Amblycipitidae appeared as the most basal extant lineage within the Siluriformes, while the Bagridae appeared as the sister group of Cranoglanididae and Pangasiidae. The mitochondrial genome sequence of P. eupogon has been deposited in GenBank (accession No. KJ001784). PMID:27323031

  11. Ichthyofaunal Diversity and Water Quality in the Kangsabati Reservoir, West Bengal, India

    Directory of Open Access Journals (Sweden)

    Amalesh Bera

    2014-01-01

    Full Text Available The ichthyofauna in relation to water quality was studied on monthly basis from March, 2010 to February, 2011 in the Kangsabati Reservoir, West Bengal. The study revealed that physicochemical parameters of Kangsabati Reservoir were congenial for 39 fish species of commercial importance, belonging to 7 orders, 15 families, and 26 genera. The Cypriniformes were dominant with 17 species, followed by Siluriformes and Perciformes, with 7 species each, Channiformes with 3 species, Osteoglossiformes and Synbranchiformes with 2 species each, and Anguilliformes with 1 species. Regarding their conservation status, 27 species were of least concern, 1 species was vulnerable, 6 species were near threatened, 1 species was data deficient, and 4 species were not evaluated (IUCN-Version 2014.1. Economical values have also been evaluated. Water parameters such as temperature, pH, alkalinity, dissolved oxygen, hardness, free CO2, salinity, total inorganic nitrogen, and phosphate were recorded and found suitable for fish production. Conductivity, transparency, and high chloride level are minor limiting factor that may needs rectification for improved fisheries management.

  12. Mitochondrial genome sequence of Paramisgurnus dabryanus from the Yellow River estuary: implication for Cobitidae phylogeny.

    Science.gov (United States)

    Zhu, Ming; Liu, Feng

    2016-07-01

    Paramisgurnus dabryanus Sauvage (Cobitidae, Cypriniformes) is a native large-scale loach widely distributed in main inland freshwater systems of China. The complete mitochondrial genome sequence of P. dabryanus from the Yellow River (YR) estuary was characterized in the present study. The circular mitogenome is 16 570 bp in size and contains two ribosomal RNA genes, 22 transfer RNA genes, and 13 protein-coding genes. The overall A + T content is 56.64% in the range of other Cobitidae studied. The mitogenome of P. dabryanus YR has an overall nucleotide sequence identity of 96.9% with that from Zhejiang, and 89.2% with that from Hubei. All the Misgurnus and Paramisgurnus species were clustered into two Misgurnus/Paramisgurnus complex clades with high bootstrap values based on the whole mitogenome sequences. The mitochondrial phylogenomic analysis indicates that the relationships in Cobitidae are inconsistent with the current classification system and taxonomic revision is required on the basis of molecular phylogeny and morphology. PMID:26162049

  13. 清水江流域天柱段鱼类资源调查%Investigation on Fish Resources in Tianzhu Section of Qingshui River

    Institute of Scientific and Technical Information of China (English)

    孟立霞; 潘年庆; 薛明淦

    2012-01-01

    对清水江流域天柱段的鱼类资源进行了调查,结果发现:共有52种(亚种),隶属4目12科41属,其中以鲤形目种类最多,鲈形目、鲇形目次之,合鳃目最少。干流分布的鱼类种类比支流多;小型野杂鱼种类多,大中型鱼类较少。近些午来鱼类资源呈减少的趋势,主要是受过度捕捞、水利工程和水域环境污染的影响。%In Tianzhu Section of Qingshui River, freshwater fish are investigated. The results show that freshwater fish have 52 species ( subspecies ) , which belong to 4 orders, 12 families, 41genera. The Cypriniformes are the most species, Perciformes and Siluriformes are less, Symbranchir are minimal. The species in main stream are more than in tributary stream. Small wild miscellaneous fish are more than large and medium fish. In recent years the fish resources have appeared a declining trend, which mainly due to overfishing, water conservancy project and water environment pollution.

  14. Cloning and tissue distribution of appetite-regulating peptides in pirapitinga (Piaractus brachypomus).

    Science.gov (United States)

    Volkoff, H

    2015-10-01

    Pirapitinga (or red-bellied pacu, Piaractus brachypomus, Characiforme, Serrasalmidae) is an economically important South American fish for which the endocrine mechanism of the regulation of feeding has never been examined. To better understand these mechanisms, cDNAs encoding the appetite-regulating peptides orexin, cocaine- and amphetamine-regulated transcript (CART), apelin, cholecystokinin (CCK), peptide YY (PYY), leptin and ghrelin were isolated in pirapitinga and their mRNA distributions examined in peripheral tissues and brain. When compared to other fish, the sequences obtained for all peptides were most similar to those of other Characiforme fish (i.e. Mexican cavefish) and Siluriformes (catfish) as well as Cypriniformes (i.e. goldfish, zebrafish). All peptides were widely expressed within the brain. With the exception of CART, which was only expressed in brain, the mRNAs of all peptides were present in several peripheral tissues, including gastrointestinal tract, kidneys and gills. The widespread and peptide-specific distributions suggest that each peptide might have distinct physiological actions in the brain and on peripheral tissues, in particular on the gastrointestinal tract, which include feeding regulation. This preliminary study opens new avenues for further functional studies on the endocrine regulation of feeding in Serrasalmidae fish, including pirapitinga. PMID:25846408

  15. Clupeiformes' Egg Envelope Proteins characterization: The case of Engraulis encrasicolus as a proxy for stock assessment through a novel molecular tool.

    Science.gov (United States)

    Miccoli, Andrea; Leonori, Iole; Estonba, Andone; De Felice, Andrea; Piccinetti, Chiara Carla; Carnevali, Oliana

    2016-07-01

    Zona radiata proteins are essential for ensuring bactericidal resistance, oocyte nutrients uptake and functional buoyancy, sperm binding and guidance to the micropyle, and protection to the growing oocyte or embryo from the physical environment. Such glycoproteins have been characterized in terms of molecular structure, protein composition and phylogenetics in several chordate models. Nevertheless, research on teleost has not been extensive. In Clupeiformes, one of the most biologically relevant and commercially important order which accounts for over 400 species and totally contributes to more than a quarter of the world fish catch, Egg Envelope Protein (EEP) information exist only for the Clupea pallasii and Engraulis japonicus species. The European anchovy, Engraulis encrasicolus, the target of a well-consolidated fishery in the Mediterranean Sea, has been ignored until now and the interest on the Otocephala superorder has been fragmentally limited to some Cypriniformes and Gonorynchiformes, as well. The aim of the present study was to fill the ZP protein-wise gap of knowledge afflicting the understanding of the European anchovy's reproductive process and to expand the background on Clupeiformes. We cloned the five Engraulis encrasicolus' zp genes and deduced their products, determined their tissue distribution, quantified their mRNA expression throughout the reproductive cycle and provided an insight into their evolution through phylogenetic tools. Furthermore, we proposed a multivariate statistics-based method to objectively infer and/or confirm the classification of Engraulis encrasicolus' sexual maturity stages by analyzing data of zp mRNAs' relative abundance. PMID:27060425

  16. EL CASTIGO FÍSICO: EL CUERPO COMO REPRESENTACIÓN DE LA PERSONA, UN CAPÍTULO EN LA HISTORIA DELA OCCIDENTALIZACIÓN DE AMÉRICA, SIGLOS XVI-XVIII

    Directory of Open Access Journals (Sweden)

    ALEJANDRA ARAYA ESPINOZA

    2006-12-01

    Full Text Available Este trabajo se centra en dos procesos paulatinos, no necesariamente consecutivos cronológicamente, pero indispensablemente ligados entre sí: la condena del castigo físico como pena judicial y la privatización de la mortificación del cuerpo, o el paso de la coacción externa a la autocoacción. Ambos tópicos permiten entender la modernidad como proceso de privatización del cuerpo desde los gestos de "ennoblecimiento del cuerpo" y de "compasión por el cuerpo". Se revisan aspectos del debate sobre el castigo físico -como elemento significativo del proceso de occidentalización de América-, desde los discursos en la documentación judicial, en los textos jurídicos referidos a las penas aflictivas en el sistema colonial penal y el debate sobre la mortificación corporal en el ámbito religioso. Teórica y metodológicamente se trabaja desde la historia de las mentalidades y del cuerpo usando las propuestas teóricas de David Le Breton, Mary Douglas, Norbert Elias, René Girard y Serge GruzinskiThis work centres on two gradual processes that in spite of not being chronologically consecutive are necessarily joined: the penalty of the physical punishment like a judicial sentence and the privatization of the mortification of the body, or the step of the external constraint to the auto-constraint. Both topics allow to understand the modernity as process of privatization of the body from the gestures of "ennoblement of the body" and of "pity for the body ". For this purpose, we will check the debates on the physical punishment as significant element of the process of "occidentalización" of America, from the judicial speeches referred to distressing sentences in the colonial penal system and the debate on the corporal mortification in the religious area. The theoretical and methodological line centres on the history of the mentalities and of the body following the offers of David Le Breton, Mary Douglas, Norbert Elias, René Girard and Serge

  17. Solar thermal power meeting - Proceedings

    International Nuclear Information System (INIS)

    This document summarizes the presentations and debates of the first edition of the Solar thermal power meeting. Content: 1 - Opening talk (Jean-Louis BAL, SER); 2 - Solar thermal power, European and global road-maps (Cedric Philibert, IEA; Mariangels Perez Latorre, Estela); 3 - first round-table on the international development of solar energy (Philippe Lorec, DGEC France; Said Mouline, Aderee Morocco; Obaid Amrane, Masen Morocco; Kawther Lihidheb, ANME Tunisia; Abdelaziz Boumahra, Rouiba Eclairage, Algeria; Badis Derradji, NEAL Algeria; Yao Azoumah, Lesee, 2IE Foundation Burkina Faso; Mamadou Amadou Kane, MPEM Mauritania; Jean-Charles Mulet, Bertin Technologies); 4 - Second round-table on the French solar thermal offer for export (Georgina Grenon, DGEC; Stephanie Bouzigueseschmann, DG Tresor; Armand Pineda, Alstom; Florent Brunet, Mena-Areva; Roger Pujol, CNIM; Gilles David, Enertime; Michel Wohrer, Saed; Mathieu Vrinat, Sogreah; Marc Benmarraze, Solar Euromed; 5 - Presentation of Amisole - Moroccan association of solar and wind industries (Ahmed Squalli, Amisole); 6 - Third round-table on French research at the solar industry service (Gilles Flamant, Promes Lab. CNRS; Francois Moisan, Ademe; Tahar Melliti, CGI; Andre Joffre, Derbi; Michel Wohrer, Capenergies; 7 - Fourth round table on projects financing (Vincent Girard, Loan Officer BEI; Bertrand Marchais, Miga World Bank; Philippe Meunier, CDC Climat Groupe Caisse des Depots; Christian de Gromard, AFD; Laurent Belouze, Natixis; Piotr Michalowski, Loan Officer BEI); 8 - Closing of the meeting (Roger Pujol, SER)

  18. Fabrication of cationic cellulosic nanofibrils through aqueous quaternization pretreatment and their use in colloid aggregation.

    Science.gov (United States)

    Liimatainen, Henrikki; Suopajärvi, Terhi; Sirviö, Juho; Hormi, Osmo; Niinimäki, Jouko

    2014-03-15

    The aqueous pre-treatment of cellulose with periodate and Girard' reagent T was employed as a novel and promising method to promote nanofibrillation of wood pulp and to obtain cellulosic nanofibrils with cationic functionality (CNFC). To demonstrate the feasibility of CNFCs in particle aggregation, a kaolin clay model suspension was aggregated by the CNFCs. Direct high-pressure homogenization of cationized cellulose resulted in nanofibrils exhibiting typical widths of 10-50 nm and cationic charge densities ranging from 1.10 to 2.13 mequiv.g(-1). The nanofibril suspensions existed in the form of highly transparent gels and possessed cellulose I crystalline structures. All of the CNFCs promoted strong aggregation of kaolin and produced voluminous kaolin-CNFC aggregates with lateral dimensions of several millimeters. Moreover, the CNFCs maintained good aggregation performance through wide pH (3-9) and temperature (25-60 °C) ranges. Thus, CNFCs were shown to be highly potential candidates for replacement of present synthetic soluble flocculation and coagulation aids. PMID:24528718

  19. [History of human epidemic and endemic diseases in the southwest Indian Ocean].

    Science.gov (United States)

    Gaüzère, B-A; Aubry, P

    2013-05-01

    Smallpox has been known in the Mascarene Islands since 1729, and in 1898, the vaccinogenic and anti-rabies Institute of Tananarive, the future Pasteur Institute of Madagascar, was created to combat it. Cholera first arrived in the Mascarenes in 1819, but did not affect the Comoros Islands and Madagascar until the current pandemic. Bubonic plague has beset the ports of Madagascar and the Mascarenes since 1898. Girard and Robic developed the anti-plague vaccine in 1931 at the Pasteur Institute of Madagascar. The Mascarenes lost their reputation as Eden when malaria arrived in 1841, and this disease remains prominent in Madagascar and Comoros. Leprosy has been known in La Réunion since 1726 and is still very present in Mayotte, Anjouan, and Madagascar. Leptospirosis is a public health problem, except in Madagascar and the Comoros. Dengue, chikungunya, and Rift Valley fever are also present. HIV/AIDS is not a major concern, except in Mauritius, where it was spread by injection drug use, in the Seychelles and in Madagascar's largest cities. Madagascar is the principal site worldwide of chromoblastomycosis, first described there in 1914. PMID:23797833

  20. Chemical Analysis of Organic Aerosols Using Reactive Nanospray Desorption Electrospray Ionization Mass Spectrometry

    Science.gov (United States)

    Laskin, A.; Laskin, J.; Nizkorodov, S.

    2013-12-01

    Nanospray Desorption Electrospray Ionization (nano-DESI) technique integrated with high resolution mass spectrometry (HR-MS) enables molecular level analysis of organic aerosol (OA) samples. In nano-DESI, analyte is desorbed into a small volume solvent bridge formed between two capillaries positioned in contact with analyte and enables fast and efficient characterization of OA collected on substrates without sample preparation. We report applications of the nano-DESI/HR-MS approach in a number of our recent studies focused on molecular identification of organic compounds in laboratory and in field collected OA samples. Reactive nano-DESI approach where selected reagent is added to the solvent is used for examining the presence of individual species containing specific functional groups and for their quantification within complex mixtures of OA. Specifically, we use the Girard's reagent T (GT) to probe and quantify carbonyl compounds in the SOA mixtures. We estimate for the first time the amounts of dimers and trimers in the SOA mixtures. We found that the most abundant dimer in limonene/O3 SOA was detected at the ˜0.5 pg level and the total amount of dimers and trimers in the analyzed sample was ˜11 pg. Understanding of the OA composition at the molecular level allowed us to identify key aging reactions, including the transformation of carbonyls to imines and carbonyl-imine oligomerization, that may contribute to the formation of brown carbon in the atmosphere.

  1. Violencia y ética práctica

    Directory of Open Access Journals (Sweden)

    Fermin Roland Schramm

    2009-01-01

    Full Text Available El texto parte de la constatación de que el fenómeno de la violencia es un tema poco estudiado en bioética y se propone delinear las condiciones de posibilidad de tal estudio a partir de un doble desafío: a el epistemológico, de inteligibilidad y comprensión de la violencia como fenómeno real y "complejo"; b el normativo y ético, del control y tratamiento de este fenómeno "oscuro". Comienza con un breve análisis conceptual del término violencia, destacando las características consideradas pertinentes para la bioética. En un segundo momento, aborda su historia conceptual, distinguiendo tres momentos de la historia de la filosofía: I la fase arcaica o mitopoiética; II la fase filosófica o racional y III la fase contemporánea o híbrida, en la cual se presentan las contribuciones de René Girard, Emmanuel Lévinas y Judith Butler. Concluye señalando la necesidad de una definición adecuada de la categoría violencia para que se torne un objeto de la bioética, como son los actos humanos voluntarios de agentes morales competentes que usan una fuerza injustificada para imponer a pacientes morales sufrimientos evitables y no consentidos.

  2. Macroinvertebrate response to acid mine drainage: community metrics and on-line behavioural toxicity bioassay

    International Nuclear Information System (INIS)

    The hypothesis is tested that toxicity of acid mine drainage can be detected by a selection of existing macroinvertebrate community and bioindicator metrices supplemented by toxicity tests with the local mosquitofish Gambusia holbrooki Girard and the shrimp Atyaephyra desmaresti Millet. The behavioural responses of A. desmaresti to acid mine drainage were recorded in the Multispecies Freshwater Biomonitor[reg], based on behaviour and survival as parameters. Bioassessment methods were based on community diversity, structure, function, and bioindicators and supplemented by chemical analysis (temperature, pH, metals). The Biological Monitoring Working Party adapted for the Iberian Peninsula, the number of predators (Coleoptera, Hemiptera) and the number of Ephemeroptera and Trichoptera taxa differentiated the sites well. The on-line toxicity test revealed pH-dependent acute toxicity of the acid mine drainage for the shrimp (LC50-48 h: pH-AMD=5.8) and a pH- dependent decrease in locomotory activity with the lowest-observed-response-times (LORTs) within 5 h of exposure. Shrimp were more sensitive to acid mine drainage than fish (LC50-48 h: pH-AMD=4.9). A new multimetric index combining toxicity testing and bioassessment methods is proposed. - Toxicity of acid mine drainage was evaluated by macroinvertebrate bioassessment and a new on-line rapid behavioural toxicity test with Atyaephyra desmaresti (Crustacea)

  3. Influence of salinity and prey presence on the survival of aquatic macroinvertebrates of a freshwater marsh

    Science.gov (United States)

    Kang, Sung-Ryong; King, Sammy L.

    2012-01-01

    Salinization of coastal freshwater environments is a global issue. Increased salinity from sea level rise, storm surges, or other mechanisms is common in coastal freshwater marshes of Louisiana, USA. The effects of salinity increases on aquatic macroinvertebrates in these systems have received little attention, despite the importance of aquatic macroinvertebrates for nutrient cycling, biodiversity, and as a food source for vertebrate species. We used microcosm experiments to evaluate the effects of salinity, duration of exposure, and prey availability on the relative survival of dominant aquatic macroinvertebrates (i.e., Procambarus clarkii Girard, Cambarellus puer Hobbs, Libellulidae, Dytiscidae cybister) in a freshwater marsh of southwestern Louisiana. We hypothesized that increased salinity, absence of prey, and increased duration of exposure would decrease survival of aquatic macroinvertebrates and that crustaceans would have higher survival than aquatic insect taxon. Our first hypothesis was only partially supported as only salinity increases combined with prolonged exposure duration affected aquatic macroinvertebrate survival. Furthermore, crustaceans had higher survival than aquatic insects. Salinity stress may cause mortality when acting together with other stressful conditions.

  4. Solar thermal power meeting - Proceedings; Les Rencontres du Solaire Thermodynamique - Actes

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-07-15

    This document summarizes the presentations and debates of the first edition of the Solar thermal power meeting. Content: 1 - Opening talk (Jean-Louis BAL, SER); 2 - Solar thermal power, European and global road-maps (Cedric Philibert, IEA; Mariangels Perez Latorre, Estela); 3 - first round-table on the international development of solar energy (Philippe Lorec, DGEC France; Said Mouline, Aderee Morocco; Obaid Amrane, Masen Morocco; Kawther Lihidheb, ANME Tunisia; Abdelaziz Boumahra, Rouiba Eclairage, Algeria; Badis Derradji, NEAL Algeria; Yao Azoumah, Lesee, 2IE Foundation Burkina Faso; Mamadou Amadou Kane, MPEM Mauritania; Jean-Charles Mulet, Bertin Technologies); 4 - Second round-table on the French solar thermal offer for export (Georgina Grenon, DGEC; Stephanie Bouzigueseschmann, DG Tresor; Armand Pineda, Alstom; Florent Brunet, Mena-Areva; Roger Pujol, CNIM; Gilles David, Enertime; Michel Wohrer, Saed; Mathieu Vrinat, Sogreah; Marc Benmarraze, Solar Euromed; 5 - Presentation of Amisole - Moroccan association of solar and wind industries (Ahmed Squalli, Amisole); 6 - Third round-table on French research at the solar industry service (Gilles Flamant, Promes Lab. CNRS; Francois Moisan, Ademe; Tahar Melliti, CGI; Andre Joffre, Derbi; Michel Wohrer, Capenergies; 7 - Fourth round table on projects financing (Vincent Girard, Loan Officer BEI; Bertrand Marchais, Miga World Bank; Philippe Meunier, CDC Climat Groupe Caisse des Depots; Christian de Gromard, AFD; Laurent Belouze, Natixis; Piotr Michalowski, Loan Officer BEI); 8 - Closing of the meeting (Roger Pujol, SER)

  5. Environmental parameters affecting the structure of leaf-litter frog (Amphibia: Anura communities in tropical forests: a case study from an Atlantic Rainforest area in southeastern Brazil

    Directory of Open Access Journals (Sweden)

    Carla C. Siqueira

    2014-04-01

    Full Text Available Despite a recent increase of information on leaf litter frog communities from Atlantic rainforests, few studies have analyzed the relationship between environmental parameters and community structure of these animals. We analyzed the effects of some environmental factors on a leaf litter frog community at an Atlantic Rainforest area in southeastern Brazil. Data collection lasted ten consecutive days in January 2010, at elevations ranging between 300 and 520 m above sea level. We established 50 quadrats of 5 x 5 m on the forest floor, totaling 1,250 m² of sampled area, and recorded the mean leaf-litter depth and the number of trees within the plot, as well as altitude. We found 307 individuals belonging to ten frog species within the plots. The overall density of leaf-litter frogs estimated from the plots was 24.6 ind/100m², with Euparkerella brasiliensis (Parker, 1926, Ischnocnema guentheri (Steindachner, 1864, Ischnocnema parva (Girard, 1853 and Haddadus binotatus (Spix, 1824 presenting the highest estimated densities. Among the environmental variables analyzed, only altitude influenced the parameters of anuran community. Our results indicate that the study area has a very high density of forest floor leaf litter frogs at altitudes of 300-500 m. Future estimates of litter frog density might benefit from taking the local altitudinal variation into consideration. Neglecting such variation might result in underestimated/overestimated values if they are extrapolated to the whole area.

  6. Scientific Programme Committee

    Science.gov (United States)

    2013-02-01

    Scientific Programme Committee A. Blondel, University of Geneva A. Cervera, IFIC M. Dracos, IN2P3 I. Efhymiopoulos, CERN J. Ellis, CERN S. Geer, FNAL R. Garoby, CERN M. Goodman, ANL D. Harris, FNAL T. Hasegawa, KEK P. Huber, Virginia Tech. D. Kaplan, IIT Y.D. Kim, Sejong University H. Kirk, BNL Y. Kuno, Osaka University K. Long, Imperial College N.K. Mondal, TIFR J. Morfin, FNAL Y. Mori, Kyoto University K. Nishikawa, KEK V. Palladino, University of Napoli C. Prior, RAL F.J.P. Soler, University of Glasgow J. Strait, FNAL R. Svoboda, University of California Davis F. Terranova, LN Frascati M. Zisman, LBNL Local Organizing Committee E. Benedetto, CERN/NTUA C. Blanchard, University of Geneva A. Blondel, University of Geneva (co-chair) I. Efthymiopoulos, CERN (co-chair) F. Dufour, University of Geneva F. Girard-Madoux, CERN E. Gschwendtner, CERN A. Korzenev, University of Geneva M. Morer-Olafsen, CERN S. Murphy, University of Geneva G. Prior, CERN G. Wikström, University of Geneva E. Wildner, CERN Sponsors EuCARD European Organization for Nuclear Research (CERN) Swiss Institute for Particle Physics (CHIPP) University of Geneva

  7. [The plague: disease and vaccine?].

    Science.gov (United States)

    Michel, P; Rasoamanana, B; Rasolofonirina, N; Roux, J

    1992-01-01

    Plague has existed in Madagascar since 1896, with epidemic control achieved by GIRARD with an EV vaccine in 1937. Plague persists in Madagascar, however, due to the large animal reservoir. With a predilection for nodal tissues, Yersinia pestis is a virulent bacteria that is potent inducer of antibody synthesis. Immunity mechanisms stimulated by infection were studied: 1. In human by immunoenzymatic methods 2. In mice by seroprotection and vaccinating tests. Induced immunity for people in endemic and endemic-epidemic areas, is significant, affecting approximately 70% of these populations. In non endemic areas, immunity is found in only 33% of the population, perhaps this explains the persistence of epidemic? In all cases, this immunity is a quick onset (6 days), is persistent (> 2 years), and has demonstrable serious recognition of YOP (Yersinia Outer Proteins) by Western Blot method. Human antibodies were shown to be protective for mice. Animals vaccinated by YOP were protected equally well, when compared to animals infected with Yersinia pestis and subsequently treated with antibiotics. Finally, YOP aerosols were also shown to induce antibodies. In conclusion, plague is a vaccinatable bacterial disease and YOP can be used as an animal vaccine to permit plague control in the rat reservoir. PMID:1345094

  8. Occurrence of the Toxin-Producing Aspergillus versicolor Tiraboschi in Residential Buildings.

    Science.gov (United States)

    Piontek, Marlena; Łuszczyńska, Katarzyna; Lechów, Hanna

    2016-01-01

    In an area representative of a moderate climate zone (Lubuskie Province in Poland), mycological tests in over 270 flats demonstrated the occurrence of 82 species of moulds. Aspergillus versicolor Tiraboschi was often encountered on building partitions (frequency 4: frequently). The ability to synthesize the carcinogenic sterigmatocystin (ST) means that it poses a risk to humans and animals. Biotoxicological tests of biomasses of A. versicolor were conducted in the Microbiological and Toxicological Laboratory, using the planarians Dugesia tigrina (Girard). The obtained results of the tests covered a broad range of toxicity levels of isolated strains: from weakly toxic (100-1000 mg·L(-3)) to potently toxic (1-10 mg·L(-3)). The high-performance liquid chromatography (HPLC) physicochemical method confirmed the ability of A. versicolor strains to synthesize sterigmatocystin. All of the samples of the air-dry biomasses of the fungi contained ST in the range between 0.03 and 534.38 mg·kg(-1). In the bio-safety level (BSL) classification A. versicolor belongs to category 1. Additionally, A. versicolor is an allergenic mould. PMID:27589778

  9. Un apocalipsis perverso: reflexiones en torno a la violencia simbólica en Eloy

    Directory of Open Access Journals (Sweden)

    Pablo Concha Ferreccio

    2014-01-01

    Full Text Available El artículo propone una lectura de la violencia simbólica en la novela Eloy (1960, de Carlos Droguett. Se comienza analizando el vínculo entre Eloy y las mujeres para presentar la estructura psíquica de la perversión. Luego se aborda la violencia simbólica a la luz de la teoría de René Girard sobre el motivo literario de los hermanos. Habría dos dobles de Eloy: el policía y el barbero. La relación de ambos con el bandido rural se caracteriza por su naturaleza perversa, que funciona del mismo modo: la violencia es caracterizada como violencia estatal, contenida y secular, que denuncia la existencia de un lazo social reificado entre campo y ciudad. Se termina ensayando una explicación para este doble uso de la perversión desde el contexto de producción de la novela.

  10. June 2014 Phoenix pulmonary journal club: new therapies for IPF and EBUS for sardoidosis

    Directory of Open Access Journals (Sweden)

    Mathew M

    2014-06-01

    Full Text Available No abstract available. Article truncated at 150 words. Richeldi L, du Bois RM, Raghu G, Azuma A, Brown KK, Costabel U, Cottin V, Flaherty KR, Hansell DM, Inoue Y, Kim DS, Kolb M, Nicholson AG, Noble PW, Selman M, Taniguchi H, Brun M, Le Maulf F, Girard M, Stowasser S, Schlenker-Herceg R, Disse B, Collard HR; INPULSIS Trial Investigators. Efficacy and safety of nintedanib in idiopathic pulmonary fibrosis. N Engl J Med. 2014;370(22:2071-82. Nintedanib is a tyrosine kinase inhibitor that has been shown to decrease the decline of FVC in phase 2 trials of idiopathic pulmonary fibrosis (IPF. This study was a phase 3 trial in which 2 replicate trials comparing nintedanib 150 mg twice daily to placebo. The trials were randomized double blind placebo controlled performed over 205 sites in 24 countries. Inclusion criteria were an age > 40, FVC > 50% and DLCO 30-79%. Patients were excluded if on prednisone > 15 mg/day or any other ...

  11. Hydrologic Properties of Aquifers in the Central Savannah River Area

    Energy Technology Data Exchange (ETDEWEB)

    Snipes, D.S.; Benson, S.M.; Price Jr., Van; Temples, T.J.

    1996-01-02

    The hydrologic properties of selected aquifer systems underlying the Milhaven and Girard sites in Georgia were determined through a series of aquifer performance tests performed from October, 1994 to January, 1995. At the Milhaven site, the systems under investigation consisted of the upper, middle and lower components of the Upper Floridan, the lower Dublin, and the lower Midville aquifers. At the Dublin site, only the lower Dublin and lower Midville aquifers were tested. In addition, the hydrologic properties of the lower Midville aquifer underlying the P, B and D Areas at the Savannah River Site were determined by a series of aquifer tests conducted in 1993 and 1994. The tests generally consisted of collecting water level and atmospheric data for 24 hours followed by a 72 hour pump test and a subsequent 72 hour recovery period. These tests were designed to determine the aquifer properties over a large area, to determine whether any hydrologic boundaries existed in the area, and to find out if leakance could be induced through the confining units which separated the aquifer units.

  12. [A contribution to the history of understanding the epidemiology of plague in Madagascar].

    Science.gov (United States)

    Blanchy, S

    1995-01-01

    Plague appeared in Madagascar in 1898, the pandemic coinciding with the French conquest. Until 1921, harbor epidemics occurred in Tamatave, Majunga, Diégo-Suarez, Fort-Dauphin, Vatomandry. In 1921, probably favored by the building of roads and railways, plague takes root on the High Lands where it becomes endemic above 800 meters. The vaccine achievement by Girard and Robic with the EV strain, and its mass application from 1935 by Estrade, Milliau, Brault, Seyberlich and Jan Keguistel, allowed to control the disease. The D.D.T. and sulfamids discovery makes the urban epidemics almost disappear, allowing it to subsist as only rural sporadic or familial cases with a low mortality. The mass vaccination can be stopped in 1959. Since 1988 the diseases incidence has been increasing, probably in relation with the quasi disappearance of deinsectisation and antibiotics. Nevertheless, urban epidemics are still rare and limited in a parallel direction to the substitution, in the city, of Rattus rattus, main reservoir and victim of the disease, by Rattus norvegicus, less sensitive to the infection. PMID:11625936

  13. Bloedoffers en morele vorming: Gewelddadigheid as faset van Christelike tradisies

    Directory of Open Access Journals (Sweden)

    Pieter J.J. Botha

    2008-09-01

    Full Text Available Blood sacrifice and moral formation: Violence as a facet of Christian traditions Is it possible that the discourse (and the corresponding assumptions of blood sacrifice contribute to violent behavior? After a brief review of the pervasive presence of blood sacrifice language in formative Christianity, some theoretical perspectives on the concept of sacrifice are discussed. Attention is given to traditional views emphasizing sacrifice as transaction and communication, as well as to the theories of René Girard and Walter Burkert. These theoretical reflections remind us of how interwoven sacrifice and our cultural histories; sacrifice is rooted in coping strategies for powerful and dangerous emotions and events. Some connections between blood sacrifice imagery and sacrificial talk and the possible substantiation and naturalization of violent actions and values are indicated. Sacrifice language is not the only cause of violent behavior, but it does contribute to the cultural scripts of communities, promotes egocentric values, maintains magical components in worldview and facilitates the perception of violence as a commodity.

  14. BRAIN 2.0: Time and Memory Complexity Improvements in the Algorithm for Calculating the Isotope Distribution

    Science.gov (United States)

    Dittwald, Piotr; Valkenborg, Dirk

    2014-04-01

    Recently, an elegant iterative algorithm called BRAIN ( Baffling Recursive Algorithm for Isotopic distributio N calculations) was presented. The algorithm is based on the classic polynomial method for calculating aggregated isotope distributions, and it introduces algebraic identities using Newton-Girard and Viète's formulae to solve the problem of polynomial expansion. Due to the iterative nature of the BRAIN method, it is a requirement that the calculations start from the lightest isotope variant. As such, the complexity of BRAIN scales quadratically with the mass of the putative molecule, since it depends on the number of aggregated peaks that need to be calculated. In this manuscript, we suggest two improvements of the algorithm to decrease both time and memory complexity in obtaining the aggregated isotope distribution. We also illustrate a concept to represent the element isotope distribution in a generic manner. This representation allows for omitting the root calculation of the element polynomial required in the original BRAIN method. A generic formulation for the roots is of special interest for higher order element polynomials such that root finding algorithms and its inaccuracies can be avoided.

  15. Physiological hepatic response to zinc oxide nanoparticle exposure in the white sucker, Catostomus commersonii.

    Science.gov (United States)

    Dieni, Christopher Anthony; Callaghan, Neal Ingraham; Gormley, Patrick Thomas; Butler, Kathryn Marie Alison; Maccormack, Tyson James

    2014-05-01

    Liver toxicity of commercially relevant zinc oxide nanoparticles (nZnO) was assessed in a benthic freshwater cypriniform, the white sucker (Catostomus commersonii). Exposure to nZnO caused several changes in levels of liver enzyme activity, antioxidants, and lipid peroxidation end products consistent with an oxidative stress response. Aconitase activity decreased by ~65% but tended to be restored to original levels upon supplementation with Fe(2+), indicating oxidative inactivation of the 4Fe-4S cluster. Furthermore, glucose-6-phosphate dehydrogenase activity decreased by ~29%, and glutathione levels increased by ~56%. Taken together, these suggest that nZnO induces hepatic physiological stress. Each assay was then validated by using a single liver homogenate or plasma sample that was partitioned and treated with nZnO or Zn(2+), the breakdown product of nZnO. It was found that Zn(2+), but not nZnO, increased detected glutathione reductase activity by ~14% and decreased detected malondialdehyde by ~39%. This indicates that if appreciable nZnO dissolution occurs in liver samples during processing and assay, it may skew results, with implications not only for this study, but also for a wide range of nanotoxicology studies focusing on nZnO. Finally, in vitro incubations of cell-free rat blood plasma with nZnO failed to generate any significant increase in malondialdehyde or protein carbonyl levels, or any significant decrease in ferric reducing ability of plasma. This suggests that at the level tested, any oxidative stress caused by nZnO is the result of a coordinated physiological response by the liver. PMID:24704545

  16. Gonadotropin-Releasing hormones in the brain and pituitary of the white sucker

    Science.gov (United States)

    Robinson, T. Craig; Tobet, Stuart A.; Chase, Cindy; Waldron, Travis; Sower, Stacia A.

    2000-01-01

    The present study investigated GnRH forms within the brain of a representative of the order Cypriniformes, the white sucker, Catostomus commersoni, using HPLC, RIA, andimmunocytochemistry. Several immunoreactive (ir) GnRH forms were identified in the brain of the white sucker by chromatography and radioimmunoassay, including ir-salmon GnRH, ir-lamprey GnRH-I and -III, and ir-chicken GnRH-II. Results from immunocytochemical studies were consistent with multiple GnRH forms distributed in different patterns, particularly for fibers. Neuronal perikarya containing ir-salmon GnRH and ir-lamprey-like GnRH were found laterally within the preoptic area and rostralhypothalamus. Cells containing exclusively ir-salmon GnRH appeared slightly more rostrally, but in the same region. Fibers containing ir-salmon GnRH and ir-lamprey-like GnRH were seen throughout the caudal telencephalon and extended into thediencephalon, toward the pituitary. Fibers containing ir-chicken-II-like GnRH were also seen in the caudal telencephalon, but were concentrated more dorsally in the diencephalon. Within the pituitary, fibers containing ir-salmon GnRH and ir-lamprey-like GnRH entered the neurohypophysis, but differed in their destinations. Fibers containing ir-salmon GnRH remained within the neurohypophysis, while fibers containing ir-lamprey-like GnRH targeted adenohypophyseal tissue. These findings are consistent with the hypothesis that multiple GnRH forms with multiple functions exist within the brain and pituitary of teleosts and provide further evidence of a lamprey-like GnRH within an early evolved teleost species.

  17. Fish remnants from the excavations of the Bronze Age barrow near Maryanskoe village (Dnepropetrovsk region, Ukraine

    Directory of Open Access Journals (Sweden)

    O. M. Kovalchuk

    2014-10-01

    Full Text Available The Bronze Age mound (2.5–2.3 kya BC is located near the Maryanskoe village (Apostolovskyi district, Dnepropetrovsk region and was excavated in 1953. The results of determination of the fish remnants, which were found during the excavation, are presented in the paper. Eleven species belonging to 9 genera, 5 families and 5 orders (Acipenseriformes, Cypriniformes, Siluriformes, Esociformes, Perciformes were identified: russian sturgeon Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833, stellate sturgeon A. stellatus Pallas, 1771, common ide Idus idus (Linnaeus, 1758, common roach Rutilus rutilus (Linnaeus, 1758, pontic roach R. frisii (Nordmann, 1840, common bream Abramis brama (Linnaeus, 1758, common carp Cyprinus carpio Linnaeus, 1758, tench Tinca tinca (Linnaeus, 1758, european catfish Silurus glanis Linnaeus, 1758, northern pike Esox lucius (Linnaeus, 1758, and zander Sander lucioperca (Linnaeus, 1758. Most of them are quite common in the Dnieper river basin. It was found that carp fishes predominate in the number of species. Most of the bone remnants in the collection belong to zander, catfish and pike, while common roach, pontic roach and common bream are identified by the few bones. This may indicate a different role of these species in the diet of the local population. The ratio of skeletal elements in the collection is the evidence of the fish cutting on the site. Body length and weight was reconstructed for 64 fish specimens. It was found that they were mature and small-sized, except for catfish, pike and perch. Taking into account the characteristics of the funeral rituals of the Yamna culture population, fish bones from the mound near Maryanskoe can be remnants of the parting meal.

  18. Independently evolved upper jaw protrusion mechanisms show convergent hydrodynamic function in teleost fishes.

    Science.gov (United States)

    Staab, Katie Lynn; Holzman, Roi; Hernandez, L Patricia; Wainwright, Peter C

    2012-05-01

    A protrusible upper jaw has independently evolved multiple times within teleosts and has been implicated in the success of two groups in particular: Acanthomorpha and Cypriniformes. We use digital particle image velocimetry (DPIV) to compare suction feeding flow dynamics in a representative of each of these clades: goldfish and bluegill. Using DPIV, we contrast the spatial pattern of flow, the temporal relationship between flow and head kinematics, and the contribution of jaw protrusion to the forces exerted on prey. As expected, the spatial patterns of flow were similar in the two species. However, goldfish were slower to reach maximal kinematic excursions, and were more flexible in the relative timing of jaw protrusion, other jaw movements and suction flows. Goldfish were also able to sustain flow speeds for a prolonged period of time as compared with bluegill, in part because goldfish generate lower peak flow speeds. In both species, jaw protrusion increased the force exerted on the prey. However, slower jaw protrusion in goldfish resulted in less augmentation of suction forces. This difference in force exerted on prey corresponds with differences in trophic niches and feeding behavior of the two species. The bluegill uses powerful suction to capture insect larvae whereas the goldfish uses winnowing to sort through detritus and sediment. The kinethmoid of goldfish may permit jaw protrusion that is independent of lower jaw movement, which could explain the ability of goldfish to decouple suction flows (due to buccal expansion) from upper jaw protrusion. Nevertheless, our results show that jaw protrusion allows both species to augment the force exerted on prey, suggesting that this is a fundamental benefit of jaw protrusion to suction feeders. PMID:22496281

  19. Evolution of dopamine receptor genes of the D1 class in vertebrates.

    Science.gov (United States)

    Yamamoto, Kei; Mirabeau, Olivier; Bureau, Charlotte; Blin, Maryline; Michon-Coudouel, Sophie; Demarque, Michaël; Vernier, Philippe

    2013-04-01

    The receptors of the dopamine neurotransmitter belong to two unrelated classes named D1 and D2. For the D1 receptor class, only two subtypes are found in mammals, the D1A and D1B, receptors, whereas additional subtypes, named D1C, D1D, and D1X, have been found in other vertebrate species. Here, we analyzed molecular phylogeny, gene synteny, and gene expression pattern of the D1 receptor subtypes in a large range of vertebrate species, which leads us to propose a new view of the evolution of D1 dopamine receptor genes. First, we show that D1C and D1D receptor sequences are encoded by orthologous genes. Second, the previously identified Cypriniform D1X sequence is a teleost-specific paralog of the D1B sequences found in all groups of jawed vertebrates. Third, zebrafish and several sauropsid species possess an additional D1-like gene, which is likely to form another orthology group of vertebrate ancestral genes, which we propose to name D1E. Ancestral jawed vertebrates are thus likely to have possessed four classes of D1 receptor genes-D1A, D1B(X), D1C(D), and D1E-which arose from large-scale gene duplications. The D1C receptor gene would have been secondarily lost in the mammalian lineage, whereas the D1E receptor gene would have been lost independently in several lineages of modern vertebrates. The D1A receptors are well conserved throughout jawed vertebrates, whereas sauropsid D1C receptors have rapidly diverged, to the point that they were misidentified as D1D. The functional significance of the D1C receptor loss is not known. It is possible that the function may have been substituted with D1A or D1B receptors in mammals, following the disappearance of D1C receptors in these species. PMID:23197594

  20. Révison caryologique des barbinés et hypothèses concernant la plésiomorphie possible de l'état polyploïde chez les Cyprinidés

    Directory of Open Access Journals (Sweden)

    COLLARES-PEREIRA M. J.

    1994-07-01

    Full Text Available Le modèle d'évolution caryologique des Cyprinidés, selon lequel le nombre polyploïde 2n=100 doit être considéré comme plésiomorphe pour la famille, en opposition à la théorie orthodoxe, semble se vérifier par la publication récente de nouvelles données concernant les nombres chromosomiques de Cyprininés d'Afrique et d'Asie du sud. L'hypothèse d'un ancêtre de type Cyprininé, appartenant probablement à une lignée proche des Barbinés, ainsi que l'existence d'une polyploïdie fonctionnant comme un «système-ouvert» est suggérée sur la base de caractères caryoévolutifs. L'importance de ce phénomène dans le processus évolutif de quelques lignées justifie un appel à l'inclusion future de l'information caryologique dans les travaux de systématique évolutive. En effet, les progrès actuels en cytogénétique moléculaire, en rendant possible la reconnaissance d'homologies chromosomiques interspécifiques, permettront probablement de clarifier les relations phylogénétiques au sein même des Cypriniformes.

  1. The impact of mining upon the features of the Blue Lagoon Lake in the Aghireşu area

    Directory of Open Access Journals (Sweden)

    Gavril Pandi

    2009-04-01

    Full Text Available The substrate of Aghireşu area contains several resources, which in time it was exploited and processed in locality. Among them is the brown coal, the gypsum, the kaolin. The exploitations began in galleries, and then passed to phase of surface quarries. After mining was formed several lakes trough the collapse of the galleries; to them was added the artificial lakes too. The Blue Lagoon Lake is a hydrological unit, the most representative of mixed origin. The water characteristics of the lake are under the influence of mineral composition of the substrate, the activity of mining, the erosion processes, the characteristics of lakes depressions, the climate elements, etc. The water physical and chemical properties are analyzed in space and time scale variation. Sampling and analysis made afford to compare the values in horizontally and vertically plain. The paper is part of a larger scale study for some genes` (Cytb, ND4L and D-loop nucleotidic structure identification by sequencing, to distinguish the structural differences and their exact length inase pairs. Research was carried out on individuals of Carassius gibelio (Bloch, 1782 (Actinopterygii,Cypriniformes from two different populations, Iezăreni and Movileni (Iaşi, from which dorsal musculartissue was sampled. Mitochondrial DNA (mtDNA isolation and purification was carried out automaticallyusing Promega’s Maxwell 16 (SEV module. Cytochrome b (cytb was multiplied by a two stage>polymerase chain reaction (PCR, using two sets of complementary primers (1 set for each fragment.Direct sequencing of PCR products revealed that the cytochrome b has one sequence of 1140bp. Theobtained sequences were subsequently compared with sequences of the same gene from otherindividuals within this species, towards identifying possible differences in the nucleotidic structure.Key Words: Carassius, cytocrhome b, mtDNA.

  2. Chromosome Evolution and Genome Miniaturization in Minifish

    Science.gov (United States)

    Liu, Shaojun; Hui, Tan Heok; Tan, Sze Ley; Hong, Yunhan

    2012-01-01

    Background Paedocypris is a newly established genus of fish in Southeast Asia. Paedocypris is characterized by several unique features, including a tiny adult size (thus named miniature fish or minifish), fragmentary habitats of acidic peat blackwater swamps, an unusual reproduction mode and truncated development. These peculiarities lend themselves excellent for studying chromosome evolution and rapid speciation in vertebrates but also make them highly controversial for the phylogenetic position. Methodology and Principal Findings We have established an organ procedure to prepare chromosome spreads from tiny organs of minifish and performed a cytogenetic study on two species of the genus Paedocypris, namely P. carbunculus (Pc) and P. sp. “Singkep” (Ps). We found 30 and 34 chromosomes in diploid cells of Pc and Ps, respectively, which are unusual in teleost fishes. The diploid metaphase has 5 pairs of metacentrics and 7 pairs of subtelocentrics in Pc compared to 3 pairs of metacentrics and 11 pairs of subtelocentrics in Ps, whereas the haploid metaphase contains 5 metacentrics and 7 subtelocentrics in Pc compared to 3 metacentrics and 11 subtelocentrics Ps. Chromosome behavior in first meiosis revealed the presence of a chromosomal ring consisting of 2 metacentrics in Pc, suggesting that centric fusion rather than fission was responsible for the karyotypic evolution from Ps to Pc. Flow cytometry revealed that Pc had a 45% nuclear staining intensity relative to medaka whose genome is 700 Mb in size and contains 0.81 pg DNA. The Pc genome should have 315 Mb in length and 0.36 pg of DNA, which represent one of the smallest values in vertebrates, suggesting genome miniaturization in this organism. Conclusions Our data demonstrate that gross chromosome rearrangements and genome miniaturization have accompanied the evolution of Paedocypris fishes. Our data also place Paedocypris outside currently described taxa of the Cypriniformes. PMID:22615970

  3. Composition and changes in abundance and biomass of fish assemblages along the Jingjiang section of the Yangtze River over the last decade

    Directory of Open Access Journals (Sweden)

    Shasha Sun

    2013-11-01

    Full Text Available The Jingjiang section of the Yangtze River, which lies near the estuary with an irregular semidiurnal tide, is the National Aquatic Germplasm Resource Reserve and plays an important role in conserving fishery resources. To clarify the composition and changes in abundance/biomass of fish assemblages of these coastal waters, investigations were carried out along the Jingjiang section of the Yangtze River in the autumns of 2002 to 2011. Results showed that 91 species, belonging to 12 orders and 23 families, were collected from surveys conducted during this period. Fish included 47 species of Cypriniformes, 17 species of Perciformes and 10 species of Siluriformes. Four were migratory species, 13 were estuarine, and 74 were freshwater fish. The average number of different fish species recorded each autumn was 47 (range = 32–61, and mean Jaccard similarity index was 53.72 (range = 38.46–69.09%. The total number of dominant and common species recorded was 42, with the annual value averaging 19. Dominant and common species were mainly small fish, such as Hemiculter bleekeri, Pseudobrama simony, Pseudobagrus nitidus etc., as well as juveniles of large or medium fish, such as Culter alburnus, Parabramis pekinensis, P. vachellii etc. The mean individual weight (MIW of the fish assemblage was 6.99 g. The abundance biomass comparison curve indicated that the first dominant species recorded in autumn in all years except 2010 were all very small-sized fish. The most dominant species in 2002–2009 were also of small-size fish, and large-size fish only contributed to the dominant species in 2010 and 2011. Our research also indicated a general increase in abundance or biomass (CPUEN、CPUEW, the MIW of the fish assemblage, and the population size of 62% of dominant and common species. This suggested that fishery resources of the waters studied are likely to improve.

  4. First Record of Transversotrema Witenberg, 1944 (Digenea) from the Americas, with Comments on the Taxonomy of Transversotrema patialense (Soparkar, 1924) Crusz and Sathananthan, 1960, and an Updated List of Its Hosts and Geographic Distribution.

    Science.gov (United States)

    Womble, Matthew R; Cox-Gardiner, Stephanie J; Cribb, Thomas H; Bullard, Stephen A

    2015-12-01

    Specimens of Transversotrema patialense (sensu lato) ( Soparkar, 1924 ) Crusz and Sathananthan, 1960 (Digenea: Transversotrematidae) infected the skin (epidermal spaces beneath scales near pectoral fins) of 4 of 126 (prevalence 3%; mean intensity 1.8) zebrafish ( Danio rerio (Hamilton, 1822) [Cypriniformes: Cyprinidae]) purchased in 2009 and cultured by a California (USA) fish supplier. These fish were sold as "laboratory-reared" and "specific pathogen free," purportedly raised in a recirculating aquaculture system that included zebrafish only. We herein describe the morphological features of this transversotrematid using light and scanning electron microscopy, provide a comprehensive list of hosts (snails and fishes) and geographic locality records for specimens reported as T. patialense, which is perhaps a species complex, and provide a brief historical synopsis of the taxonomic and life history research that has been conducted on this fluke. No species of Transversotrema previously had been reported from the Americas; however, this discovery is not surprising given that: (1) a suitable intermediate host (red-rimmed melania, Melanoides tuberculata (Müller, 1774) [Cerithioidea: Thiaridae]) has been established in California and elsewhere in North America, (2) the zebrafish is a susceptible definitive host, and (3) T. patialense reportedly matures on a broad ecological and phylogenetic spectrum of freshwater fishes. To our knowledge, this is the northern-most geographic locality record for a species of this genus. We suspect this case study represents an example of a parasite that may now be established in North America by the fortuitous co-occurrence of a susceptible, exotic snail host (the red-rimmed melania) and a susceptible, widely distributed, exotic fish host (the zebrafish). PMID:26335181

  5. Scolex morphology of monozoic cestodes (Caryophyllidea) from the Palaearctic Region: a useful tool for species identification.

    Science.gov (United States)

    Oros, Mikulás; Scholz, Tomás; Hanzelová, Vladimíra; Mackiewicz, John S

    2010-03-01

    A comparative study of the scoleces of caryophyllidean tapeworms (Cestoda: Caryophyllidea), parasitic in cypriniform fishes in the Palaearctic Region, was carried out using light and scanning electron microscopy. Three-dimensional pictures of the scoleces of 18 species of caryophyllidean cestodes of the Capingentidae (1 species), Caryophyllaeidae (7) and Lytocestidae (10), and outlines of the scoleces and anterior extent of the testes and vitelline follicles of 19 Palaearctic taxa were documented. Both species of Atractolytocestus Anthony, 1957 possess a bulboacuminate scolex, whereas species of Archigetes Leuckart, 1876 have fossate scoleces of the bothrioloculodiscate type, with loculi, bothrium-like depressions and an apical disc. Breviscolex orientalis Kulakovskaya, 1962, the only member of the Capingentidae, has a cuneiform scolex, as do both taxa of the lytocestid genus Caryophyllaeides Nybelin, 1922. The scoleces of two species of Caryophyllaeus Gmelin, 1790 are flabellate, whereas that of the congeneric C. fimbriceps Annenkova-Chlopina, 1919 is cuneicrispitate. Khawia Hsü, 1935, the most specious Palaearctic genus, with seven taxa that we consider to be valid, has the highest diversity in scolex morphology: semi-bulbate, flabellate, cuneiform, cuneifimbriate, truncated cuneiform-flabellate and festoon-like. Species of Monobothrium Nybelin, 1922 have either a digitiform scolex with widened posterior part or cuneiform, with lateral auricular extensions. Paracaryophyllaeus gotoi (Motomura, 1927) is characteristic in its possessing a bulbate scolex, whereas Paraglaridacris limnodrili (Yamaguti, 1934) has a fossate scolex of the bulboloculate type with bothrium-like depressions and feebly developed lateral loculi. Anterior extent of the testes and vitelline follicles and their mutual position show a somewhat higher variability than scolex shape, with intraspecific variation in some taxa, such as Atractolytocestus sagittatus (Kulakovskaya et Akhmerov, 1965), B

  6. Biological characteristics of crucian by quantitative inspection method

    Science.gov (United States)

    Chu, Mengqi

    2015-04-01

    Biological characteristics of crucian by quantitative inspection method Through quantitative inspection method , the biological characteristics of crucian was preliminary researched. Crucian , Belongs to Cypriniformes, Cyprinidae, Carassius auratus, is a kind of main plant-eating omnivorous fish,like Gregarious, selection and ranking. Crucian are widely distributed, perennial water all over the country all have production. Determine the indicators of crucian in the experiment, to understand the growth, reproduction situation of crucian in this area . Using the measured data (such as the scale length ,scale size and wheel diameter and so on) and related functional to calculate growth of crucian in any one year.According to the egg shape, color, weight ,etc to determine its maturity, with the mean egg diameter per 20 eggs and the number of eggs per 0.5 grams, to calculate the relative and absolute fecundity of the fish .Measured crucian were female puberty. Based on the relation between the scale diameter and length and the information, linear relationship between crucian scale diameter and length: y=1.530+3.0649. From the data, the fertility and is closely relative to the increase of age. The older, the more mature gonad development. The more amount of eggs. In addition, absolute fecundity increases with the pituitary gland.Through quantitative check crucian bait food intake by the object, reveals the main food, secondary foods, and chance food of crucian ,and understand that crucian degree of be fond of of all kinds of bait organisms.Fish fertility with weight gain, it has the characteristics of species and populations, and at the same tmes influenced by the age of the individual, body length, body weight, environmental conditions (especially the nutrition conditions), and breeding habits, spawning times factors and the size of the egg. After a series of studies of crucian biological character, provide the ecological basis for local crucian's feeding, breeding

  7. Exploring the Jupiter's and Saturn's radiation belts with LOFAR

    Science.gov (United States)

    Girard, Julien N.; Zarka, Philippe; Pater Imke, de; Hess, Sebastien; Tasse, Cyril; Courtin, Regis; Hofstadter, Mark; Santos-Costa, Daniel; Nettelmann, Nadine; lorenzato, Lise

    2014-05-01

    Since its detection in the mid-fifties, the decimeter synchrotron radiation (DIM), originating from the radiation belts of Jupiter, has been extensively observed over a wide spectrum (from >300 MHz to 22 GHz) by various radio instruments (VLA, ATCA, WSRT, Cassini...). They provided accurate flux measurements as well as resolved maps of the emission that revealed spatial, temporal and spectral variabilities. The strong magnetic field (~4.2 G at the equator) is responsible for the radio emission generated by relativistic electrons. The emission varies at different time scales (short-time variations of hours to long-term variation over decades) due to the combination of visibility configuration (fast rotating 'dipole' magnetic field, beamed radio emission) and intrinsic local variations (interaction between relativistic electrons and satellites/dust, delayed effect of the solar wind ram pressure, impacts events) (e.g. de Pater & Klein, 1989; de Pater & Dunn, 2003; Bagenal (ed.) et al., 2004; Santos-Costa, 2009, 2011). A complete framework is necessary to fully understand the source, loss and transport processes of the electrons populating the inner magnetosphere over a wide frequency range. The low frequencies are associated with electron of lower energies situated in weaker magnetic field regions. LOFAR, the LOw Frequency ARray (LOFAR) (van Haarlem et al., 2012), the last generation of versatile and digital ground-based radio interferometer operates in the [30-250] MHz bandwidth. It brings very high time (~μsec), frequency (~kHz) and angular (~asec) resolutions and huge sensitivities (~mJy). In November 2011, a single 10-hour track enabled to cover an entire planetary rotation and led to image, for the first time, the radiation belts between 127-172 MHz (Girard et al. 2012, 2013). In Feb 2013, an 11-hour joint LOFAR/WSRT observing campaign seized the dyname state of the radiation belts from 45 MHz up to 5 GHz. We will present the current study of the radiation belts

  8. El matrimonio de Tobías y la sexualidad: un estudio psicológico

    Directory of Open Access Journals (Sweden)

    Cristián Barría Iroumé

    2005-01-01

    Full Text Available El libro de Tobías del Antiguo Testamento es un relato novelado que trata de la vida familiar, la muerte y la sexualidad. Hay un caso de matrimonio vinculado a la muerte: una joven desespera después de haber perdido sucesivamente siete maridos en la noche de bodas. Dios se apiada y la ayudará a través de un ángel. Un joven, Tobías, con su virtud la salvará del maleficio. Este relato ha tenido un profundo impacto en la concepción de la sexualidad y del matrimonio cristiano en la historia, asociándosele con una actitud de moderación sexual inicial (las noches de Tobías. Se analiza el relato utilizando conceptos antropológicos y psicoanalíticos: los ritos de paso; la curación milagrosa; el temor de la iniciación sexual y la noción de víctima expiatoria según R. Girard. Es el relato de un pueblo antiguo que elabora religiosamente sus temores ante la muerte y el sexo. Se encuentran en el texto elementos de transición desde una concepción mítica y mágica arcaica hasta una concepción más evolucionada de religiosidad. Se contrasta su visión moralizante del sexo con la visión más libre y optimista del CantarThe book of Tobit from the Old Testament is a novelized narrative that treats of family life, death and sexuality. There is a case of matrimony linked to death: a young woman becomes desperate after having lost successively seven husbands on their wedding nights. God has pity on her, and helps her through an angel. A young man, Tobit, will virtuously save her from the curse. This story has had a profound impact on the concept of sexuality and of Christian marriage in history, associating itself with an attitude of initial sexual moderation (Tobit's nights. This narrative is analyzed in this article by utilizing anthropological and psychoanalytical concepts: the rites of passage; the miraculous healing; the fear of sexual initiation and the notion of the expiatory victim according to R. Girard. It is the story of an ancient

  9. Influência dos fatores abióticos e da disponibilidade de presas sobre comunidade de serpentes do Planalto Médio do Rio Grande do Sul Influence of abiotic factors and availability of prey on the occurrence of snakes in the middle plateau region of Rio Grande do Sul, Brazil

    Directory of Open Access Journals (Sweden)

    Noeli Zanella

    2009-03-01

    Full Text Available A influência dos fatores abióticos sobre a disponibilidade de presas e a dieta das espécies de serpentes mais abundantes do Planalto Médio do Rio Grande do Sul, foi estudada em duas áreas: floresta e campo. O trabalho foi desenvolvido utilizando serpentes coletadas com os métodos: procura limitada por tempo, encontros ocasionais, armadilhas de interceptação e queda, e serpentes depositadas na coleção de répteis da Universidade de Passo Fundo. Foram registradas as guildas alimentares das seis espécies mais abundantes: anurófagas (n = 2: Echinanthera cyanopleura (Cope, 1885 e Thamnodynates cf. strigatus (Günther, 1858; rodentívoras (n = 1: Bothrops alternatus Duméril, Bibron & Duméril, 1854; moluscófagas (n = 1: Tomodon dorsatus Duméril, Bibron & Duméril, 1854 e generalistas (n = 2: Liophis poecilogyrus (Wied-Neuwied, 1825 e Philodryas patagoniensis (Girard, 1858. Dos fatores abióticos analisados, a abundância de serpentes foi mais relacionada à temperatura máxima (R² = 0,66 e não apresentou relação significativa com a pluviosidade. A abundância de anfíbios apresentou relação positiva com a pluviosidade (R² = 0,54 e não foi significativa com a temperatura mínima. A abundância de serpentes não foi correlacionada com a abundância de anfíbios e roedores.The influence of abiotic factors on the availability of prey and on the diet of the most abundant snake species in the middle plateau region of Rio Grande do Sul was studied in two areas: forest and field. Snakes captured using time-constrained search, occasional encounters and pitfall traps and snakes deposited in the reptile collection of the University of Passo Fundo were studied. The feeding guilds of the six most abundant species were recorded: anurophagous (n = 2: Echinanthera cyanopleura (Cope, 1885 and Thamnodynates cf. strigatus (Günther, 1858; rodent-eating (n = 1: Bothrops alternatus Duméril, Bibron & Duméril, 1854; molluscophagous (n = 1: Tomodon

  10. Long-term alteration of Rousse rock samples for CO2 storage: an experimental and numerical study

    Science.gov (United States)

    Bachaud, P.; Sissmann, O.; Parra, T.

    2013-12-01

    CO2 injection into the Rousse depleted gas reservoir, located in the south-west of France, started in January 2010 and 50,000 tons of this greenhouse effect gas were ultimately injected until March 2013 (Prinet et al. 2013). CO2 fate in the reservoir represents a problem of primary importance, that have been studied by several complementary research works led in collaboration by Total and IFPEN (e.g. Girard et al. 2013, Chiquet et al. 2013). One of the aspects covered by these studies concerned geochemical reactions between the injected fluid, reservoir water and rock minerals. A long-term CO2-exposure experiment was conducted in pressure vessels, and allowed the ageing of reservoir mini-plugs at in situ temperature and pressure conditions (150°C and 80 bar) during almost 24 months. Solid characterization was led every 2 to 3 months using an X-ray microprobe. Elementary maps were then acquired and converted into mineralogical maps using a statistical analysis based on pixels composition. This method allowed the identification of main minerals and of their evolution with alteration. Solution elementary composition was analyzed every week using an ICP-MS. These compositions were used for speciation calculations using the IFPEN's geochemical code Arxim. Initial reservoir mineralogy is mainly constituted of two types of dolomites. One of which forms the rock matrix, the other fills the main reservoir fractures. Quartz, pyrite and calcite traces, as well as two types of phyllosilicates (a potassic illite and a magnesian mixture of chlorite and mica) were also evidenced thanks to our analytical method. After some months of alteration, mineralogical evolutions were observed at the sample edge. Magnesian carbonates (dolomite and magnesite) seemed to precipitate, while an increase of iron, sodium and sulphur (probably due to anhydrite formation) was detected. The combination of these experimental observations to numeric speciation and minerals Saturation Index (SI

  11. Using meteorological ensembles for atmospheric dispersion modelling of the Fukushima nuclear accident

    Science.gov (United States)

    Périllat, Raphaël; Korsakissok, Irène; Mallet, Vivien; Mathieu, Anne; Sekiyama, Thomas; Didier, Damien; Kajino, Mizuo; Igarashi, Yasuhito; Adachi, Kouji

    2016-04-01

    Dispersion models are used in response to an accidental release of radionuclides of the atmosphere, to infer mitigation actions, and complement field measurements for the assessment of short and long term environmental and sanitary impacts. However, the predictions of these models are subject to important uncertainties, especially due to input data, such as meteorological fields or source term. This is still the case more than four years after the Fukushima disaster (Korsakissok et al., 2012, Girard et al., 2014). In the framework of the SAKURA project, an MRI-IRSN collaboration, a meteorological ensemble of 20 members designed by MRI (Sekiyama et al. 2013) was used with IRSN's atmospheric dispersion models. Another ensemble, retrieved from ECMWF and comprising 50 members, was also used for comparison. The MRI ensemble is 3-hour assimilated, with a 3-kilometers resolution, designed to reduce the meteorological uncertainty in the Fukushima case. The ECMWF is a 24-hour forecast with a coarser grid, representative of the uncertainty of the data available in a crisis context. First, it was necessary to assess the quality of the ensembles for our purpose, to ensure that their spread was representative of the uncertainty of meteorological fields. Using meteorological observations allowed characterizing the ensembles' spread, with tools such as Talagrand diagrams. Then, the uncertainty was propagated through atmospheric dispersion models. The underlying question is whether the output spread is larger than the input spread, that is, whether small uncertainties in meteorological fields can produce large differences in atmospheric dispersion results. Here again, the use of field observations was crucial, in order to characterize the spread of the ensemble of atmospheric dispersion simulations. In the case of the Fukushima accident, gamma dose rates, air activities and deposition data were available. Based on these data, selection criteria for the ensemble members were

  12. The liver isoform of carnitine palmitoyltransferase 1 is not targeted to the endoplasmic reticulum.

    Science.gov (United States)

    Broadway, Neil M; Pease, Richard J; Birdsey, Graeme; Shayeghi, Majid; Turner, Nigel A; David Saggerson, E

    2003-01-01

    Liver microsomal fractions contain a malonyl-CoA-inhibitable carnitine acyltransferase (CAT) activity. It has been proposed [Fraser, Corstorphine, Price and Zammit (1999) FEBS Lett. 446, 69-74] that this microsomal CAT activity is due to the liver form of carnitine palmitoyltransferase 1 (L-CPT1) being targeted to the endoplasmic reticulum (ER) membrane as well as to mitochondria, possibly by an N-terminal signal sequence [Cohen, Guillerault, Girard and Prip-Buus (2001) J. Biol. Chem. 276, 5403-5411]. COS-1 cells were transiently transfected to express a fusion protein in which enhanced green fluorescent protein was fused to the C-terminus of L-CPT1. Confocal microscopy showed that this fusion protein was localized to mitochondria, and possibly to peroxisomes, but not to the ER. cDNAs corresponding to truncated (amino acids 1-328) or full-length L-CPT1 were transcribed and translated in the presence of canine pancreatic microsomes. However, there was no evidence of authentic insertion of CPT1 into the ER membrane. Rat liver microsomal fractions purified by sucrose-density-gradient centrifugation contained an 88 kDa protein (p88) which was recognized by an anti-L-CPT1 antibody and by 2,4-dinitrophenol-etomoxiryl-CoA, a covalent inhibitor of L-CPT1. Abundance of p88 and malonyl-CoA-inhibitable CAT activity were increased approx. 3-fold by starvation for 24 h. Deoxycholate solubilized p88 and malonyl-CoA-inhibitable CAT activity from microsomes to approximately the same extent. The microsomal fraction contained porin, which, relative to total protein, was as abundant as in crude mitochondrial outer membranes fractions. It is concluded that L-CPT1 is not targeted to the ER membrane and that malonyl-CoA CAT in microsomal fractions is L-CPT1 that is derived from mitochondria, possibly from membrane contact sites. PMID:12401113

  13. The Use of Some Hydrazine Derivatives in the Separation and Microdetermination of Aluminum

    International Nuclear Information System (INIS)

    The economic importance of aluminum metal pushed us to evaluate a rapid and accurate method for its separation and determination. The study introduces Girard's reagent P(HAPH) as a good chelating agent for the flotation of Al(III). The different parameters affecting the flotation separation process are investigated in detail. It was found that 1x10-4 mol/L HAPH is sufficient to separate 2.5x10-5 mol/L Al(III) using 1x10-3 mol/L oleic acid as a surfactant at ph ≡ 6 and at room temperature (25 degree C) with ≡ 100% efficiency. The effect of foreign ions is also studied and the data obtained show that most of the investigated cations and anions have no adverse effect on the flotation efficiency of Al(III). The decrease in the efficiency in the presence of some heavy metals may be due to the formation of metal oleates. The flotation procedure is successfully applied to remove 2.5x10-5 mol/L of Al(III) added to some natural samples obtained from different locations. Trials are made to suggest the mechanism of flotation depending on the data obtained from the physicochemical studies on the solid complexes isolated in the absence and presence of oleic acid. The use of HAPH was extended to the spectrophotometric microdetermination of Al(III) in the presence of K;[Fe(CN)6] , PFC , giving AI-HAPH-PFC compound with blue colour. This gives a new spectrophotometric method for determining 2x10-4 mol/L of Al(lll) at 730 nm with molar absorptivity of 3.42x103 L mol-1 cm-1 . The different analytical parameters are investigated

  14. [The plague reviewed by way of molecular biology].

    Science.gov (United States)

    Carniel, E

    1999-12-01

    The aetiology of plague was first discovered during the third pandemic of the disease occurring in Hong Kong in 1894. After Alexandre Yersin had identified the causal agent (Y. Pestis), Paul-Louis Simond proved the flea's role as vector. These discoveries were of prime importance for the subsequent development of efficient means for fighting plague as well as for preventive and curative treatments and vaccines. Vaccination brought about a sharp decrease in plague mortality and morbidity. However, the disease has never been eradicated. It is still prevalent in various Asian, African and American countries and is among the re-emerging diseases at the present time. The genetic basis of transmission mechanisms and pathogenicity of the bacillus are only beginning to be understood. We now know that the attenuation of the EV76 strain used by Girard and Robic as an anti-plague vaccine in Madagascar is due to the spontaneous excision of a large chromosomal DNA fragment of 102 kb, a part of which contains a group of genes implicated in the pathogenicity and appropriately called high pathogenicity island. These mechanisms of flea bacillus transmission are also beginning to be known. Two bacterial loci participating in the blocking of the ectoparasite's proventriculus have been identified. One is situated next to the high pathogenicity island on the unstable 102 kb chromosomal fragment, the other--on the large 95 kb plasmid specific to Y. pestis. The molecular basis of the bacillus' acquisition of multi-resistance to antibiotics have likewise recently been characterised. However, although Y. pestis is one of the most pathogenic micro-organisms of the bacterial world, the mechanisms responsible for this high level of pathogenecity have still not been identified. This is well worth noting, since a certain number of genes acting as pathogenicity factors in other species are present but altered in Y. pestis. Plague still withholds many secrets. PMID:11000953

  15. The origin of ethics and social order in a society without state power.

    Science.gov (United States)

    Yamamoto, K

    1999-06-01

    How ethics and social order in a society without state power had originated and developed is one of enigmas which human beings have tried to solve for a long time. Several theories on the origin of social order have been proposed since the "Social Contract" theory of Thomas Hobbes. According to Hobbes, as a society without state power is in a condition called war, a social contract among men is the origin of social order in a society where every man is against every man. Rousseau says that when human beings reach the stage in which they live in a permanent neighborhood, a property system is introduced. Then, too much ambition and avarice of man who has possessions compel him to propose the formation of a political institution, providing social order which enables him to keep his possessions. According to Nietzsche, the principle of equilibrium, that is, an eye for an eye, a tooth for a tooth is an important concept for the oldest theory of low and morality as well as the basis of justice. The sense of superiority and nobility which a strong man brave enough to take revenge feels is the origin of the antithesis "good" and "bad". Girard says that the sacred violence wielded by the community to sacrifice a surrogate victim brings about social order in a society without state power. All the aforementioned theories seem to have failed to find out that a society without state power has its own ethics that had spontaneously developed on the pagan culture. Previously, I indicated that a society without state power or a society where state power cannot function well, such as the tribal society in northern Albania, has ethics which is based on the ancient concepts of "Guest-god", "food (commensality)" and "blood". In the present paper, I propose a new theory on the origin of ethics and social order, using the model of ethics of the Kanun. PMID:10402726

  16. Mimetic desire and scapegoat mechanism in sport

    Directory of Open Access Journals (Sweden)

    Jernej Pisk

    2012-12-01

    Full Text Available BACKGROUND: The most fundamental question about sport is what is sport, what is its origin and its essence? Because sport is connected with the human being (there is no sport without human beings different anthropological visions of human being result in different understandings of sport. OBJECTIVE: The objective of this paper is to present and explain an anthropological vision of the human being and society as was developed by René Girard. In his view mimetic desire and the scapegoat mechanism have a central role in any culture, religion or other secular institutions. The explanatory power of his theory is presented when it is applied to the world of sport. METHODS: Our methodology is philosophical, involving conceptual analysis and the application of the outcomes to sport. RESULTS: In the paper we show that mimetic desire can be recognized as one of the important origins of recreational and competitive sports. When people recognize what other people are able to do or accomplish in sport this invokes the mimetic desire as a result of which motivation for sport and competiveness can arise. But mimetic rivalry leads to an unstable situation. Therefore a second element is needed: Scapegoating in sport is presented as a mean to preserve the good reputation of sport, to keep peace in sport as well as in society as a whole. Finally, the attempt to overcome mimetic desire and scapegoating in sport is presented and the question if this is worth trying at all is opened. CONCLUSIONS: The theories of mimetic desire and scapegoat mechanism have great explanatory power when they are applied to the field of sport. They could reveal us some hidden motives and forces which drive athletes and sport as a whole. Moreover, they exceed the world of sport and reveal the influence of sport on the whole of society.

  17. Ergosterone-coupled Triazol molecules trigger mitochondrial dysfunction, oxidative stress, and acidocalcisomal Ca2+ release in Leishmania mexicana promastigotes

    Directory of Open Access Journals (Sweden)

    Figarella K

    2015-12-01

    Full Text Available The protozoan parasite Leishmania causes a variety of sicknesses with different clinical manifestations known as leishmaniasis. The chemotherapy currently in use is not adequate because of their side effects, resistance occurrence, and recurrences. Investigations looking for new targets or new active molecules focus mainly on the disruption of parasite specific pathways. In this sense, ergosterol biosynthesis is one of the most attractive because it does not occur in mammals. Here, we report the synthesis of ergosterone coupled molecules and the characterization of their biological activity on Leishmania mexicana promastigotes. Molecule synthesis involved three steps: ergosterone formation using Jones oxidation, synthesis of Girard reagents, and coupling reaction. All compounds were obtained in good yield and high purity. Results show that ergosterone-triazol molecules (Erg-GTr and Erg-GTr2 exhibit an antiproliferative effect in low micromolar range with a selectivity index ~10 when compared to human dermic fibroblasts. Addition of Erg-GTr or Erg-GTr2 to parasites led to a rapid [Ca2+]cyt increase and acidocalcisomes alkalinization, indicating that Ca2+ was released from this organelle. Evaluation of cell death markers revealed some apoptosis-like indicators, as phosphatidylserine exposure, DNA damage, and cytosolic vacuolization and autophagy exacerbation. Furthermore, mitochondrion hyperpolarization and superoxide production increase were detected already 6 hours after drug addition, denoting that oxidative stress is implicated in triggering the observed phenotype. Taken together our results indicate that ergosterone-triazol coupled molecules induce a regulated cell death process in the parasite and may represent starting point molecules in the search of new chemotherapeutic agents to combat leishmaniasis.

  18. Absolute GPS Time Event Generation and Capture for Remote Locations

    Science.gov (United States)

    HIRES Collaboration

    The HiRes experiment operates fixed location and portable lasers at remote desert locations to generate calibration events. One physics goal of HiRes is to search for unusual showers. These may appear similar to upward or horizontally pointing laser tracks used for atmospheric calibration. It is therefore necessary to remove all of these calibration events from the HiRes detector data stream in a physics blind manner. A robust and convenient "tagging" method is to generate the calibration events at precisely known times. To facilitate this tagging method we have developed the GPSY (Global Positioning System YAG) module. It uses a GPS receiver, an embedded processor and additional timing logic to generate laser triggers at arbitrary programmed times and frequencies with better than 100nS accuracy. The GPSY module has two trigger outputs (one microsecond resolution) to trigger the laser flash-lamp and Q-switch and one event capture input (25nS resolution). The GPSY module can be programmed either by a front panel menu based interface or by a host computer via an RS232 serial interface. The latter also allows for computer logging of generated and captured event times. Details of the design and the implementation of these devices will be presented. 1 Motivation Air Showers represent a small fraction, much less than a percent, of the total High Resolution Fly's Eye data sample. The bulk of the sample is calibration data. Most of this calibration data is generated by two types of systems that use lasers. One type sends light directly to the detectors via optical fibers to monitor detector gains (Girard 2001). The other sends a beam of light into the sky and the scattered light that reaches the detectors is used to monitor atmospheric effects (Wiencke 1998). It is important that these calibration events be cleanly separated from the rest of the sample both to provide a complete set of monitoring information, and more

  19. The 1:1 N-NS protein complex of vesicular stomatitis virus is essential for efficient genome replication.

    Science.gov (United States)

    La Ferla, F M; Peluso, R W

    1989-01-01

    We studied the effect pH had on the N-NS protein complex to determine its role in vesicular stomatitis virus (VSV) genome replication, as we had previously shown that VSV genome replication in vitro requires the interaction of the viral N and NS proteins into a 1:1 complex. A previous report showed that the growth of VSV in L cells was sensitive to the pH of the environment (M. Fiszman, J. B. Leaute, C. Chany, and M. Girard, J. Virol. 13:801-808, 1974). We hypothesized that low pH might disrupt the N-NS protein complex, and so we investigated the molecular events leading to inhibition of viral RNA replication in vitro from extracts that were prepared from VSV-infected cells incubated at pH 6.6. We found that viral genome RNA synthesis in vitro was reduced when infected cells were maintained at pH 6.6. Through immunoprecipitation analysis of the viral soluble protein pool, we found that a complex that usually exists between the N and NS proteins at pH 7.4 was altered in extracts from infected cells maintained at pH 6.6, and this was responsible for the observed effects on viral replication. The effect of low pH on the N-NS protein complex could not be abolished by increasing the concentration of the altered complex, indicating that the effects is more than simply a decrease in the level of the protein complex in the cell. Our data provide additional evidence that the 1:1 N-NS protein complex, and not the N protein alone, serves as the substrate for viral RNA replication in vivo. Images PMID:2548001

  20. Towards Sustainable Lifestyles. A Variety of Lifestyles for a Post-carbon Society

    International Nuclear Information System (INIS)

    In 2009 a programme 'Re-thinking Society in a Post-carbon Society'- steered jointly by the Foresight Department of the French Ecology Ministry and the French Environment and Energy Management Agency (ADEME), was launched in France. It is still ongoing and aims to produce a final report in 2013. The idea of a transition towards a 'post- carbon' society includes four main objectives: reducing greenhouse gas emissions by 2050 to one quarter of what they were in 1990; near-autonomy with regard to carbon energies (petrol, gas, coal); an adequate capacity to adapt to climate change and, lastly, greater attention to situations of 'energy precariousness'. As part of the dossier Futuribles is devoting to this programme this month, Andreas Huber, Sebastien Girard and Pierre Le Marre lay out in this article the results of the studies they have carried out on 'sustainable urban milieus'. After a presentation of the notion of 'milieu' (based here on a segmentation using the Socio-milieusR method) and of the typology employed (nine main social milieus, three emergent milieus and 16 contrasting profiles), the authors show the extent to which individuals' carbon foot - prints vary, depending upon lifestyles, and what a determining effect these lifestyles have in the fields of housing and transport. They then specify the various factors influencing behaviour in the direction of sustain - able consumption (or not) and the different types of strategies of intervention that are likely to modify those behaviours. Lastly, they detail two targeted strategies, one aimed at the 'precarious seniors' milieu and the other at the 'eco-elite' milieu. Despite certain imperfections that remain to be sorted out in the study of sustainable urban milieus, these studies open up new perspectives for the development of sociologically targeted policies for a post-carbon transition. (authors)

  1. DA LEGITIMAÇÃO Wisnik e o futebol

    Directory of Open Access Journals (Sweden)

    Pedro Dolabela Chagas

    2012-08-01

    Full Text Available Acompanha-se a operação de legitimação do futebol comoobjeto de investigação intelectual em Veneno remédio – o futebole o Brasil, de José Miguel Wisnik. Correspondências com outrostrabalhos de legitimação semelhantes, nos estudos literários ena crítica da música pop. Após uma discussão preliminar sobrea legitimação, o artigo segue três etapas: na primeira,caracteriza-se o antagonista contra o qual Wisnik legitima ofutebol mediante o cotejo de proposições de Hannah Arendt,Gilles Lipovetski e René Girard; na segunda, através deaproximações com o universo pop, discute-se a ética dalegitimação de Wisnik, que não denega os problemas levantadoscontra o futebol, absorvendo-os, mas requalificando-os, noseu trabalho de legitimação; na terceira, vê-se como Wisniksitua na análise das especificidades do futebol o caminho parauma analítica que não o soterre sob expectativas externas àsua própria lógica de acontecimento. Ao final, localiza-se emWisnik a proposição de que o futebol expressa como futebol osproblemas que o afligem, proposição aliada a uma recusa emestabilizar um padrão valorativo que retire o futebol do fluxoda cultura para congelá-lo nalguma posição hierárquicaespecífica, seja ela baixa ou elevada.

  2. Review of Studies on the Germplasm Resources of the Schizothoracinae Fishes%裂腹鱼类种质多样性研究综述

    Institute of Scientific and Technical Information of China (English)

    代应贵; 肖海

    2011-01-01

    裂腹鱼类隶属于鲤形目鲤科裂腹鱼亚科,目前已记录有12属90种(亚种),是鲤科鱼类中为数不多的适应于亚洲中部青藏高原地区自然条件的一个自然类群.整个裂腹鱼类呈现出以青藏高原为中心的分布格局,中国是世界上裂腹鱼类种类分布最多的国家.目前,随着对鱼类种质资源研究越来越受到人们的关注,裂腹鱼类作为产地名贵经济鱼类,其种质资源已成为了研究的热点.主要从形态分类、物种多样性、地理分布、细胞水平、生化水平和分子水平等,对多年来裂腹鱼类种质资源研究方面的进展进行了综述,以期为裂腹鱼类资源的保护和利用提供基础资料.%The schizothoracinae fishes, commonly known as mountain carps, which belong to schizothoracinae, cyprinidae and cypriniforme, comprise 90 species (subspecies) and 12 genera. Almost all species in schizothoracinae are the wild species of big to middle size with highly scientific and economic value. The group of fishes are mainly distributed in the Qinghai-Tibetan plateau and its adjacent area. Especially, most of the species in schizothoracinae live in China and many are endemic to China. The germplasm resources, also biodiversity, cytology resources, means the gene pool of species. The germplasm resources can be studied by the methods of morphology, cell biology, biochemistry and molecular biology. With the development of study on the germplasm resources of fishes, the germplasm resources of the schizothoracinae fishes has become one of research foci today. This paper reviews the progress of study on the germplasm resources of the schizothoracinae fishes, including the researches in the classification, species diversity, geographic distribution, cytology, biochemistry and molecular biology of the group, so as to supply scientific data for the conservation and utilization of the schizothoracinae fishes.

  3. The fate of the duplicated androgen receptor in fishes: a late neofunctionalization event?

    Directory of Open Access Journals (Sweden)

    Haendler Bernard

    2008-12-01

    Full Text Available Abstract Background Based on the observation of an increased number of paralogous genes in teleost fishes compared with other vertebrates and on the conserved synteny between duplicated copies, it has been shown that a whole genome duplication (WGD occurred during the evolution of Actinopterygian fish. Comparative phylogenetic dating of this duplication event suggests that it occurred early on, specifically in teleosts. It has been proposed that this event might have facilitated the evolutionary radiation and the phenotypic diversification of the teleost fish, notably by allowing the sub- or neo-functionalization of many duplicated genes. Results In this paper, we studied in a wide range of Actinopterygians the duplication and fate of the androgen receptor (AR, NR3C4, a nuclear receptor known to play a key role in sex-determination in vertebrates. The pattern of AR gene duplication is consistent with an early WGD event: it has been duplicated into two genes AR-A and AR-B after the split of the Acipenseriformes from the lineage leading to teleost fish but before the divergence of Osteoglossiformes. Genomic and syntenic analyses in addition to lack of PCR amplification show that one of the duplicated copies, AR-B, was lost in several basal Clupeocephala such as Cypriniformes (including the model species zebrafish, Siluriformes, Characiformes and Salmoniformes. Interestingly, we also found that, in basal teleost fish (Osteoglossiformes and Anguilliformes, the two copies remain very similar, whereas, specifically in Percomorphs, one of the copies, AR-B, has accumulated substitutions in both the ligand binding domain (LBD and the DNA binding domain (DBD. Conclusion The comparison of the mutations present in these divergent AR-B with those known in human to be implicated in complete, partial or mild androgen insensitivity syndrome suggests that the existence of two distinct AR duplicates may be correlated to specific functional differences that may be

  4. Early Pliocene Sturgeons and Bony Fishes from the Dniester Valley (Republic of Moldova / Осетровые и костистые рыбы раннего плиоцена из долины Днестра (Республика Молдова

    Directory of Open Access Journals (Sweden)

    Kovalchuk О. М.

    2015-02-01

    Full Text Available В статье описаны ископаемые остатки рыб из отложений раннего плиоцена долины Днестра (Республика Молдова. Из место- нахождений Никольское и Уютное идентифицированы семь видов, относящихся к 6 родам, 4 се- мействам и 4 отрядам (Acipenseriformes, Cypriniformes, Siluriformes, Esociformes. Все они являют- ся морфологическими аналогами рецентных таксонов. В статье представлен краткий обзор раз- вития пресноводной ихтиофауны в бассейне Нижнего Днестра на протяжении раннего плиоцена.

  5. 莫莫格国家级自然保护区的鱼类多样性%Fish Diversity in Momoge National Nature Reserve

    Institute of Scientific and Technical Information of China (English)

    杨富亿; 吕宪国; 娄彦景; 娄晓楠; 薛滨; 姚书春; 肖海丰; 王波

    2012-01-01

    2008~2010年,在鱼类资源调查的基础上,分析了莫莫格国家级自然保护区的鱼类多样性特征.调查结果显示,莫莫格国家级自然保护区有鱼类物种5目11科42属49种,其中本区土著种5目10科38属45种,包括中国特有种4种,中国易危种5种,冷水种8种,国家Ⅱ级保护动物1种.土著种群落中,有鲤形目(Cypriniformes)鱼类32种,鲤科(Cyprinidae)鱼类29种,鮈亚科(Gobioninae)鱼类13种;古北界北方区黑龙江亚区黑龙江分区的物种为22种;源于东部平原和北方平原生态类群的鱼类有27种;适应于江—湖缓流环境和淡水定居生活的鱼类有33种;草上产卵和水层产卵繁殖的鱼类有37种:杂食性和初级肉食性种类有28种,它们均相对占优势.莫莫格国家级自然保护区与嫩江的鱼类群落物种结构Jaccard系数和Morisita-Hom相似性指数分别为0.918和0.838;稀有种(包括中国易危种)和常见种分别为14种和25种;物种多度格局近似于对数正态分布.这些结果表明,莫莫格国家级自然保护区的鱼类多样性与嫩江密切相关,鱼类区系复杂,物种组成与生态类型多样;群落结构相对较稳定,多样性程度相对较高,但仍面临着稀有种(包括中国易危种)尚占有一定比例而导致群落结构脆弱的潜在威胁.保持嫩江鱼类群落结构的稳定,对维护和发展莫莫格国家级自然保护区的鱼类多样性具有特别重要的意义.%Engaged in sample survey by 57 surveys with gill net, trawl net, trap net and pull net, the samples of 6 542 fish individuals were collected in Momoge National Nature Reserve during 2008-2010. The characteristics of the fish diversity in Momoge National Nature Reserve were analyzed. It was observed that there were 49 species/subspecies of 42 genus, 11 families, and 5 orders. Four species of these were introduced species while the others were indigenous, including 4 species peculiar and 5 vulnerable to China, and 8 of

  6. Biomass valorisation, a new dynamics for French agriculture. Colloquium proceedings

    International Nuclear Information System (INIS)

    programme (J. Siret, Agrice); 14 - Vegetable chemistry: from research to development (J. Barbier Valagro); 15 - Vegetable chemistry (M.E. Borredon); 16 - Biomass: a new stake (M. Girard, Pronovial)

  7. Nuclear works. Book of abstracts

    International Nuclear Information System (INIS)

    the conception of a LILW repository (Marc Mormont, Anne Bergmans), The contribution of Social Sciences and Humanities to the scientific program for radioactive waste management of Andra (Luis Aparicio), The public expert and the nuclear catastrophe (Christine Fassert); 5 - Nuclear governance: Did Fukushima put an end to nuclear revival? A post-Fukushima debates analysis in Finnish, French and British media (Markku Lehtonen), Nuclear secrecy at the test of the right to participation (Philippe Billet), Nuclear science, politics and national construction: what remains from Nehru's India in these times of uncertainty? (Berenice Girard); 6 - Working in the nuclear industry. Training and work collectives: Nuclear industry: a workers-less world? (Pierre Fournier), Ambiguity dynamics at NPPs, a pluri-disciplinary approach (Nicolas Lot), Sino-French nuclear engineering curriculums: what kind of innovation configuration? (Richard Marion). This document brings together the French and English abstracts of the different talks

  8. Ecology of Ischnocnema parva (Anura: Brachycephalidae at the Atlantic Rainforest of Serra da Concórdia, state of Rio de Janeiro, Brazil

    Directory of Open Access Journals (Sweden)

    Ana Cristina J. S. Martins

    2010-04-01

    Full Text Available Ischnocnema (Brachycephalidae includes many species that are important members of the leaf litter frog communities in the Atlantic Rainforest of Brazil. Ischnocnema parva (Girard, 1853 is endemic to the Atlantic Rainforest biome and is restricted to the forests of southeastern Brazil. Currently, the available information about the ecology of I. parva is scarce. We studied the diet, the habitat use, reproduction and density of I. parva in an area of Atlantic Rainforest at the Concórdia mountain range, Rio de Janeiro. Individuals of I. parva were captured in April 2005 using different sampling methods: time constrained search (transects, plots of 5 x 5 m (25 m² on the litter, and pitfall traps with drift fences. We found 240 frogs; 35 females and 205 males. Females (mean SVL = 19.1 mm were significantly larger (F1,238 = 143.016, R² = 0.375, p < 0.001 than males (13.2 mm. The species preyed mainly on arthropods, with ants and isopods being the most important items, both showing high values of importance index (Ix = 50.0 and 26.7, respectively. Ischnocnema parva is a terrestrial species whose preferential microhabitat at the Serra da Concórdia was the litter of the forest floor (78.7%. The activity was predominantly crepuscular-nocturnal and the estimated density of I. parva was 24.9 ind/100 m². For the eight ovigerous females captured, the mean number of mature oocytes per female was 25 (range: 22-30 and the oocyte mean diameter was 1.11 mm (N = 40 oocytes. Oocyte number increased with female body size (R² = 0.504, F1,6 = 6.107, p < 0.05, N = 8, indicating that as females increase in size they produce larger clutches. Some ecological aspects such as diet and microhabitat use were similar to that observed for an insular population of I. parva, whereas reproductive traits differed. Thus, long term studies are necessary to understand the extent to which these differences are explained by environmental factors.

  9. Yttrium-90 resin microspheres as an adjunct to sorafenib in patients with unresectable hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    Mahvash A

    2016-02-01

    Full Text Available Armeen Mahvash,1 Ravi Murthy,1 Bruno C Odisio,1 Kanwal Pratap Raghav,2 Lauren Girard,2 Sheree Cheung,1 Van Nguyen,3 Joe Ensor,4 Sameer Gadani,5 Khaled M Elsayes,6 Reham Abdel-Wahab,1,7 Manal Hassan,1 Ahmed S Shalaby,1 James C Yao,1 Michael J Wallace,1 Ahmed O Kaseb2 1Department of Interventional Radiology, 2Department of Gastrointestinal Medical Oncology, 3Department of Pharmacy, 4Department of Statistics, The University of Texas MD Anderson Cancer Center, Houston, TX, 5Department of Radiology, Saint Louis University Hospital, St Louis, MO, 6Department of Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; 7Department of Clinical Oncology, Assiut University Hospital, Assiut, Egypt Purpose: The safety and efficacy of the combined use of sorafenib and yttrium-90 resin microspheres (Y90 RMS to treat advanced hepatocellular carcinoma (HCC is not well established. We determined the incidence of adverse events with this combination therapy in patients with advanced HCC at our institution and analyzed the treatment and survival outcomes. Materials and methods: We reviewed the records of 19 patients with Barcelona Clinic Liver Cancer class B or C HCC who underwent treatment with Y90 RMS (for 21 sessions while receiving full or reduced doses of sorafenib between January 2008 and May 2010. Therapy response was evaluated using Response Evaluation Criteria in Solid Tumors. We evaluated median overall survival (OS and progression-free survival (PFS as well as hepatic and extrahepatic disease PFS and incidence of adverse events. Results: The median patient age was 67 years, and portal or hepatic venous invasion was present in eight patients (42%. Ten patients received reduced doses of sorafenib. The median Y90 radiation activity delivered was 41.2 mCi. The partial response of Response Evaluation Criteria in Solid Tumors was observed in four patients (19%. The median hepatic disease PFS was 7.82 months, extrahepatic

  10. Phase II trial of bevacizumab and erlotinib as a second-line therapy for advanced hepatocellular carcinoma

    Directory of Open Access Journals (Sweden)

    Kaseb AO

    2016-02-01

    Full Text Available Ahmed O Kaseb,1 Jeffrey S Morris,2 Michiko Iwasaki,1 Humaid O Al-Shamsi,1 Kanwal Pratap Singh Raghav,1 Lauren Girard,1 Sheree Cheung,1 Van Nguyen,3 Khaled M Elsayes,4 Lianchun Xiao,2 Reham Abdel-Wahab,1,5 Ahmed S Shalaby,1 Manal Hassan,1 Hesham M Hassabo,1 Robert A Wolff,1 James C Yao1 1Department of Gastrointestinal Medical Oncology, 2Department of Biostatistics, 3Department of Pharmacy, 4Department of Diagnostic Radiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; 5Department of Clinical Oncology, Assiut University Hospital, Assiut, Egypt Trial registry: Clinicaltrials.gov #NCT01180959. Background: Early clinical studies of bevacizumab and erlotinib in advanced hepatocellular carcinoma (HCC have a tolerable toxicity and a promising clinical outcome. We evaluated the efficacy and tolerability of this combination as a second-line therapy for HCC refractory to sorafenib. Methods: For this single-arm, Phase II study, we recruited patients with Child–Pugh class A or B liver disease, Eastern Cooperative Oncology Group performance status 0–2, and advanced HCC that was not amenable to surgical or regional therapies and treatment with sorafenib had failed (disease progressed or patient could not tolerate sorafenib. Patients received 10 mg/kg intravenous bevacizumab every 14 days and 150 mg oral erlotinib daily for 28-day cycles until progression. Tumor response was evaluated every two cycles using Response Evaluation Criteria in Solid Tumors. The primary end point was the 16-week progression-free survival rate. Secondary end points included time to progression and overall survival. Results: A total of 44 patients were enrolled and had a median follow-up time of 33.8 months (95% confidence interval [CI]: 23.5 months – not defined. The 16-week progression-free survival rate was 43% (95% CI: 28%–59%, median time to progression was 3.9 months (95% CI: 2.0–8.3 months, and median overall survival duration was 9.9 months

  11. Influencias de la densidad y podas sobre la producción de Pinus taeda L. a los 7 años de edad.

    Directory of Open Access Journals (Sweden)

    Rubén Costas

    2010-08-01

    Full Text Available En un ensayo con 3 niveles de densidad y 12 tratamientos de poda, se evaluaron los efectos de la densidad, la altura de poda, el número de levantes de poda y la interacción entre la densidad y las podas sobre la producción de la masa de Pinus taeda L. a los 7 años de edad. Las variables evaluadas fueron el diámetro a la altura de pecho medio (DAP, la altura media, el volumen individual, el volumen por unidad de superficie, el área basal, el diámetro máximo sobre muñón (DMSM, el coeficiente de forma de Girard (CFG, el diámetro de ramas (DRBC y el ángulo de inserción de las ramas (ARBC en la base de la copa. En los tratamientos con mayor densidad de plantación se observaron mayores volúmenes por unidad de superficie, área basal y CFG que en los tratamientos con menor densidad. Estos produjeron mayores DAP, volúmenes individuales, DMSM, DRBC y ARBC que los tratamientos con mayores densidades. Los tratamientos con menores alturas finales de poda produjeron mayores DAP, volúmenes individuales, área basal y volúmenes por unidad de superficie que los tratamientos con mayores alturas de poda. Los menores DMSM se obtuvieron con alturas finales de poda de 3,30 m y 4,40 m realizadas a los 4 años de edad y los mayores DMSM ocurrieron al no efectuarse podas a los 5 años hasta 5,50 m y 6,60 m. No se encontraron diferencias significativas entre los valores medios de DRBC, ARBC y CFG producidos por los 12 tratamientos de poda. La interacción entre densidad y los tratamientos de poda no fue significativa para ninguna de las variables respuestas.

  12. Spermiogenesis and spermatozoa ultrastructure in five species of the Curimatidae with some considerations on spermatozoal ultrastructure in the Characiformes

    Directory of Open Access Journals (Sweden)

    Irani Quagio-Grassiotto

    2003-09-01

    Full Text Available Spermiogenesis in the curimatid species, Steindachnerina insculpta, Cyphocharax gillii, C. modestus, C. spilotus, and Potamorhina altamazonica, is characterized by lateral development of the flagellum, nuclear rotation, eccentric nuclear fossa formation, and chromatin compacted into thick fibers. These spermatozoa exhibit a spherical head containing a nucleus with the chromatin highly condensed into thick fibers with small electron-lucent areas, and no acrosome. The nuclear fossa is of the moderate type and eccentric and penetrated by the centriolar complex. The midpiece is small, has many elongate vesicles, and a short cytoplasmic channel. Mitochondria may be elongate, branched or C-shaped, and are separated from the initial segment of the axoneme by the cytoplasmic channel. The flagellum contains the classical axoneme structure (9+2 and has a membranous compartment in the initial region; it does not have lateral fins. Only small differences were observed among the analyzed species and genera of the Curimatidae. Spermiogenesis and spermatozoa in the Curimatidae have many of the characteristics found in almost all other characiform species. On the other hand, the presence of a membranous compartment in the initial region of curimatid flagella, a structure common in many Cypriniformes spermatozoa, is unknown in other characiforms. Spermiogenesis and the spermatozoa of the Characiformes are discussed.A espermiogênese nas espécies Steindachnerina insculpta, Cyphocharax gillii, C. modestus, C. spilotus e Potamorhina altamazonica de Curimatidae é caracterizada pelo desenvolvimento lateral do flagelo, rotação do núcleo, formação excêntrica da fossa nuclear e cromatina compactada em fibras espessas. Estes espermatozóides exibem uma cabeça esférica contendo um núcleo com cromatina altamente condensada em fibras espessas com pequenas áreas eletronlúcidas, e sem acrossoma. A fossa nuclear é do tipo moderado e excêntrico, penetrada pelo

  13. Cloning and expression analysis of the insulin-like growth factor 1 in Elopichthys bambusa%鳡胰岛素样生长因子1基因的克隆及其组织特异性表达分析

    Institute of Scientific and Technical Information of China (English)

    郁建锋; 李建林; 徐建荣; 卢祥云; 柏洁; 顾志良

    2012-01-01

    The objective of the present research was to clone full-length IGFl cDNA sequence and to determine its expression in various tissues from Elopichthys bambusa. Reverse transcription PCR (RT-PCR), rapid-amplification of cDNA ends (RACE) were used to amplify full-length of IGF1 cDNA. Semi-quantatative RT-PCR was used to compare IGF1 gene expression levels from various tissues. A full-length of IGF1 cDNA sequence in E. bambusa was obtained, which included a complete open reading frame (ORF) of 486 bp, a 5 -untranslated region of 218 bp, and a 3 -untranslated region of 140 bp. The putative peplide contained a signal sequence of 44 amino acid, a mature peptide of 70 amino acid in the functional domain- and an extended carboxyl-terminal peptide of 47 amino acid in domain E. IGFl from E. bambusa showed an amino acid identity of 94. 41%-99. 38% with that from Cy print formes. The phylogenetic analyses showed that IGF1 from E. bambusa had closest relationship with other fishes in Cypriniformes. Furthermore, IGF1 mRNA levels were highest in liver but relatively low or absent in heart. A part of IGF1 promoter sequence was cloned by using Genome Walking, which included a Myogenic Differentiation Antigen (Myo D). But there was not a consensus TATA box about 5' to the start site of transcription in IGF1. These results indicated the IGF1 promoter differed to the classic promoter. These findings provide clue for IGF1 involvement in the growth and development of E. bambusa and are potential basis for its effective breeding and propagation.%为克隆鳡(Elopichys bambusa)的胰岛素样生长因子1(IGF1)全长cDNA和启动子序列及明确其组织表达特征,采用反转录PCR (RT -PCR)、cDNA末端快速扩增(RACE)和染色体步移等技术,从鳡肌肉中获得了IGF1基因的全长cDNA(844 bp)和上游启动子部分序列(627 bp).结果表明:IGF1基因包含218 bp的5 '端非翻译区、140 bp的3 '端非翻译区和486 bp的开放性阅读框(ORF),编码161

  14. 太湖鱼类群落结构及多样性%Community structure and diversity of fish in Lake Taihu

    Institute of Scientific and Technical Information of China (English)

    毛志刚; 谷孝鸿; 曾庆飞; 周露洪; 王小林; 吴林坤; 曹萍; 孙明波

    2011-01-01

    In 2009-2010, bottom trawl surveys were conducted on the fish resources in Lake Taihu, aimed to understand the characteristics of fish community structure and diversity in the Lake. A total of 50 fish species were collected, belonging to 40 genera, 15 families, and 10 orders, among which, Cypriniformes had the largest species number, accounting for 68% of the total. The fish community was composed of three ecological guilds, and limnology species were most abundant (78% of the total abundance). The dominant species were of mini-type fish such as Coilia ectenes taihuensis, Hyporhamphus intermedius, and Salangichthys jordani. As compared with historical data, the fish species number in the Lake decreased, the composition of dominant species changed greater, and the mini-type fishes with body mass <30 g were absolutely dominant , with an obvious tendency of fish resources becoming smaller-scale. Due to overfishing and water pollution, the Margalef richness index D, Shannon diversity indices H'N and H'N, and Pielou evenness indices J'N and J'w in the Lake all decreased, being 1.54, 0.21, 0.46, 0.07, and 0. 14, respectively. To some extent, the differences in the fish species composition and biodiversity reflected the adaption characteristics of the fishes to the environmental variables such as nutrients and transparency in the Lake.%为了解太湖鱼类群落结构和多样性的分布特征,于2009-2010年利用拖网等网具对该水域的鱼类资源进行了调查.结果表明:本次调查共采获鱼类50种,隶属10目15科40属,其中鲤形目种类最多,占总数的68%;鱼类生态类型以湖泊定居性种类为主,群落优势种为湖鲚(Coilia ectenes taihuensis)、间下鱵(Hyporhamphus intermedius)和陈氏短吻银鱼(Salangichthys jordani)等小型鱼类;与历史资料相比,太湖鱼类的物种数量下降,优势种组成发生较大变化,鱼类群落中体质量<30 g的小型鱼类占绝对优势,渔业资源小型化趋势明显.由

  15. 长江口九段沙湿地潮沟鱼类组成及其多样性%Fish species composition and diversity in intertidal creek of Jiuduansha wetland in the Yangtze River estuary

    Institute of Scientific and Technical Information of China (English)

    龚小玲; 张晓懿; 朱敏; 任胜杰; 孙瑛

    2011-01-01

    长江口九段沙湿地潮沟鱼类组成有着典型的长江口鱼类区系特征,为了解该湿地尤其是湿地潮沟鱼类的组成和多样性,在2009至2010年间对九段沙湿地的上沙、中沙和下沙潮沟进行了5次调查,共捕获鱼类3 637尾,105 158.9 g,42种,分别隶属于11目、22科。其中种类和个体数均以鲈形目居多,有20种,3 433尾,其次是鲤形目7种,49尾和鲱形目5种,33尾;海水、河口、淡水、洄游4种生态类型的种类所占百分比分别为26.19%、40.48%、23.81%、9.52%,而河口性鱼类的个体数和总湿重占绝对优势%The fish composition of intertidal creek of Jiuduansha wetland in the Yangtze River estuary has the typical ichthyofauna trait of Yangtze River estuary.To comprehend the fish species composition and diversity of this wetland especially the intertidal creek,we surveyed the creek of Shangsha,Zhongsha and Xiasha five times from 2009 to 2010.A total of 3 637 tails with 105 158.9 g and 42 species were captured,these species belonging to 11 orders and 22 families.The fishes number and species number of Perciformes are the highest,with 20 species,3 433 tails,next,Cypriniformes and Clupeiformes with 7 species,49 tails and 5 species,33 tails respectively.The percentage of marine species is 26.19%,estuarine species is 40.48%,freshwater species is 23.81% and diadromous species is 9.52%.The number of fishes(94.4%) and wet weight(96.3%) of estuarine species both were absolutely dominant.The catch in Shangsha,Zhongsha and Xiasha were 21 species with 742 tails 51 025.2 g,30 species with 1 007 tails 29 427.5 g and 20 species with 1 888 species 24 696.2 g respectively.Most of them were estuarine species.According to the relative density(RD) and relative significance index(IRI),it shows that Acanthogobius ommaturus,Liza haermatochiel and Eleutheronema tetradactylu were dominant species,especially the Acanthogobius ommaturus has the absolute advantage.As for diversity

  16. STUDY ON AGE, GROWTH, REPRODUCTION AND THE STATUS OF RESOURCE DEVELOPMENT OFCULTER OXYCEPHALOIDES (KREYENBERG ET PAPPENHEIM) IN THE XIAO RIVER%小江拟尖头鲌的年龄、生长、繁殖及其资源开发状况研究

    Institute of Scientific and Technical Information of China (English)

    段鹏翔; 杨志; 唐会元; 肖琼; 龚云; 肖调义; 刘小燕

    2015-01-01

    拟尖头鲌(Culter oxycephaloides)属鲤形目(Cypriniformes),鲤科(Cyprinidae),鲌亚科(Cultrinae),鲌属(Culter),为中国特有鱼类,分布于长江流域的四川、湖北、湖南等省的江河湖泊中。2013年4—7月以及10—12月在三峡库区支流小江江段收集拟尖头鲌样本1741尾,对其年龄、生长、繁殖及其资源开发状况进行了研究。结果表明:鲌所采集的拟尖头包括5个年龄组,其中2—3龄年龄组为优势年龄组,占78.59%(N=425尾);体长与体重呈幂函数关系,表达式为W =0.000007L3.10(R2=0.99,N=789尾),其生长属于匀速生长类型;采用von Bertalanffy生长方程分别拟合体长、体重与年龄的关系,其表达式分别为:Lt=519.63[1–e–0.22(t+0.12)],Wt=1806.08[1–e–0.22(t+0.12)]3.10;生长拐点年龄t=5.02龄,此时对应的体长348 mm,体重558.8 g;绝对繁殖力变动范围为20382—88430粒/尾,相对繁殖力变动范围为54—161粒/g,体重与绝对繁殖力的关系以二次方程曲线拟合度最优,其表达式为 Fabs=33462.08+0.11W–44.01W2(R2=0.49, sig.=0.02<0.05,N=31);Ⅳ卵巢卵径变动范围为0.0675—0.1325 cm,平均值为0.1060 cm;鲌拟尖头产卵类型为分批产卵类型;小江拟尖头鲌的现有开发率为E=0.51/年; Beverton-Holt动态综合模型显示,目前小江拟尖头鲌的Emax为0.40/年, E10为0.32/年, E50为0.25/年。根据研究,目前小江拟尖头鲌的种群处于过度开发状况,应采取措施,加强其资源保护。%Culter oxycephaloides (Kreyenberg et Pappenheim), which belongs to genusCulter, subfamilyCultrinae, familyCyprinidae, order Cypriniformes. They are a unique species in China and mainly live in the rivers and lakes of the Yangtze River Basin. Due to the impoundment of the Three Gorges Reservoir, the ecosystem of the upper Yangtze River basin especially the tributaries of the Three Gorges Reservoir has been profoundly changed. The Xiao River is an important tributary of the Three

  17. Book Reviews

    Directory of Open Access Journals (Sweden)

    J. van Baal

    1965-01-01

    fig. - S. Kooijman, P.K. Neuhaus, Beiträge zur Ethnographie der Pala, Mittel Neu Irland (aus dem Nachlass bearbeitet von P.C. Laufer, MSC und Carl A. Schmitz. Kölner Ethnologische Mitteilungen, Köln 1962. 452 S., 28 Abb. - J. van Baal, Peter Matthiessen, De Zonen van Nopoe; Een Kroniek uit het Steentijdperk. Vertaald uit het Engels door Beb Vuyk. J.A. Boom en Zoon, Meppel. - J. Prins, Robert R. Jay, Religion and politics in rural central Java. Cultural Report Series no. 12, Southeast Asia Studies - Yale University. VIII en 107 pagina’s, en een bibliografie. - L. Sluimers, G.I. Lewinson, Philippiny mezjdu perwoj i wtoroj mirowymi wojnami. Moskwa, Izdateljstwo wostotsjnoj literatury, 1958. (G.I. Lewinson, De Philippijnen tussen de Eerste en de tweede Wereldoorlog. Moskou, Uitgeverij voor Oosterse Literatuur, 1958. 288 blz., krt. - R.T. Zuidema, Rafael Girard, Los Mayas Eternos XIV, 493 blz., 250 foto’s en vele tekeningen, schema en 5 kaarten. Antigua Librería Robredo, Mexico D.F. 1962. - R.T. Zuidema, Bodo Spranz, Göttergestalten in den Mexikanischen Bilderhandschriften der Codex Borgia-Gruppe. Eine ikonographische Untersuchung. Acta Humboldtiana, Series Geographica et Ethnographica Nr. 4. Franz Steiner Verlag GmbH. Wiesbaden, 1964. 435 blz., 1791 fig., 32 afb., 2 tabellen.

  18. Preparation, characterization, and safety evaluation of poly(lactide-co-glycolide nanoparticles for protein delivery into macrophages

    Directory of Open Access Journals (Sweden)

    Guedj AS

    2015-09-01

    Full Text Available Anne-Sophie Guedj,1 Arnold J Kell,2 Michael Barnes,2 Sandra Stals,1 David Gonçalves,3 Denis Girard,3 Carole Lavigne11National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, 2National Research Council of Canada, Ottawa, ON, 3Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, INRS-Institut Armand-Frappier, Laval, QC, CanadaAbstract: Following infection, HIV establishes reservoirs within tissues that are inaccessible to optimal levels of antiviral drugs or within cells where HIV lies latent, thus escaping the action of anti-HIV drugs. Macrophages are a persistent reservoir for HIV and may contribute to the rebound viremia observed after antiretroviral treatment is stopped. In this study, we further investigate the potential of poly(lactic-co-glycolic acid (PLGA-based nanocarriers as a new strategy to enhance penetration of therapeutic molecules into macrophages. We have prepared stable PLGA nanoparticles (NPs and evaluated their capacity to transport an active molecule into the human monocyte/macrophage cell line THP-1 using bovine serum albumin (BSA as a proof-of-concept compound. Intracellular localization of fluorescent BSA molecules encapsulated into PLGA NPs was monitored in live cells using confocal microscopy, and cellular uptake was quantified by flow cytometry. In vitro and in vivo toxicological studies were performed to further determine the safety profile of PLGA NPs including inflammatory effects. The size of the PLGA NPs carrying BSA (PLGA-BSA in culture medium containing 10% serum was ~126 nm in diameter, and they were negatively charged at their surface (zeta potential =-5.6 mV. Our confocal microscopy studies and flow cytometry data showed that these PLGA-BSA NPs are rapidly and efficiently taken up by THP-1 monocyte-derived macrophages (MDMs at low doses. We found that PLGA-BSA NPs increased cellular uptake and internalization of the protein in vitro. PLGA

  19. The Diamondiferous Lithospheric Mantle Underlying the Eastern Superior Craton: Evidence From Mantle Xenoliths From the Renard Kimberlites, Quebec

    Science.gov (United States)

    Hunt, L.; Stachel, T.; Armstrong, J. P.; Simonetti, A.

    2009-05-01

    The Renard kimberlite cluster consists of nine pipes located within a 2km2 area in the northern Otish Mountains of Quebec. The pipes are named Renards 1 to 10, with subsequent investigation revealing Renards 5 and 6 to join at depth (now Renard 65). The pipes are located within the eastern portion of the Superior craton, emplaced into Archean granitic and gneissic host rocks of the Opinica Subprovince (Percival, 2007). Amphibolite grade metamorphism, locally passing into the granulite facies (Percival et al., 1994) occurred in late Archean time (Moorhead et al., 2003). Radiometric dating of the hypabyssal Renard 1 kimberlite indicates Neoproterozoic emplacement, with a 206Pb/238U model age of 631.6±3.5 Ma (2σ) (Birkett et al., 2004). A later study on the main phases in Renard 2 and 3 gave a similar emplacement, with a 206Pb/238U model age of 640.5±2.8Ma (Fitzgerald et al., 2008). This makes this kimberlite district one of the oldest in Canada, similar in eruption age to the Wemindji kimberlites (629±29Ma: Letendre et al., 2003). These events are broadly coeval with the conversion from subduction magmatism to rifting in northern Laurentia (Birkett et al., 2004). The bodies are part of a late Neoproterozoic to Cambrian kimberlite field in eastern Canada (Girard, 2001; Moorhead et al, 2002; Letendre et al., 2003) and fit into the north-east of the Eocambrian/Cambrian Labrador Sea Province of Heaman et al. (2004). To better understand the diamondiferous lithospheric mantle beneath the Renard kimberlites, 116 microxenoliths and xenocrysts were analysed. The samples were dominantly peridotitic, composed primarily of purple garnet, emerald green clinopyroxene and olivine, with a few pink and red garnets. A minor eclogitic component comprises predominantly orange garnets and lesser amounts of clinopyroxene. A detailed study on the major, minor and trace element composition of xenolith minerals is currently underway. All but three of the clinopyroxenes analysed to date

  20. Book reviews

    Directory of Open Access Journals (Sweden)

    Redactie KITLV

    2012-06-01

    Full Text Available The African Diaspora: A History Through Culture, by Patrick Manning (reviewed by Joseph C. Miller Atlas of the Transatlantic Slave Trade, by David Eltis & David Richardson (reviewed by Ted Maris-Wolf Abolition: A History of Slavery and Antislavery, by Seymour Drescher (reviewed by Gregory E. O’Malley Paths to Freedom: Manumission in the Atlantic World, edited by Rosemary Brana-Shute & Randy J. Sparks (reviewed by Matthew Mason You Are All Free: The Haitian Revolution and the Abolition of Slavery, by Jeremy D. Popkin (reviewed by Philippe R. Girard Fighting for Honor: The History of African Martial Arts in the Atlantic World, by T .J. Desch Obi (reviewed by Flávio Gomes & Antonio Liberac Cardoso Simões Pires Working the Diaspora: The Impact of African Labor on the Anglo-American World, 1650-1850, by Frederick C. Knight (reviewed by Walter Hawthorne The Akan Diaspora in the Americas, by Kwasi Konadu (reviewed by Ray Kea Tradition and the Black Atlantic: Critical Theory in the African Diaspora, by Henry Louis Gates Jr. (reviewed by Deborah A. Thomas From Africa to Jamaica: The Making of an Atlantic Slave Society, 1775-1807, by Audra A. Diptee (reviewed by D.A. Dunkley Elections, Violence and the Democratic Process in Jamaica 1944-2007, by Amanda Sives (reviewed by Douglas Midgett Caciques and Cemi Idols: The Web Spun by Taino Rulers between Hispaniola and Puerto Rico, by José R. Oliver (reviewed by Brian D. Bates The Latin American Identity and the African Diaspora: Ethnogenesis in Context, by Antonio Olliz Boyd (reviewed by Dawn F. Stinchcomb Reconstructing Racial Identity and the African Past in the Dominican Republic, by Kimberly Eison Simmons (reviewed by Ginetta E.B. Candelario Haiti and the Haitian Diaspora in the Wider Caribbean, edited by Philippe Zacaïr (reviewed by Catherine Benoît Duvalier’s Ghosts: Race, Diaspora, and U.S. Imperialism in Haitian Literatures, by Jana

  1. Book Reviews

    Directory of Open Access Journals (Sweden)

    Redactie KITLV

    2008-12-01

    Caribbean, 1624-1783 (Bonham C. Richardson Michaeline A. Crichlow; Negotiating Caribbean Freedom: Peasants and the State in Development (Christine Chivallon Peta Gay Jensen; The Last Colonials: The Story of Two European Families in Jamaica (Karl Watson Marc Tardieu; Les Antillais à Paris: D’hier à aujourd’hui (David Beriss Rhonda D. Frederick; “Colón Man a Come”: Mythographies of Panamá Canal Migration (Michael L. Conniff James Robertson; Gone is the Ancient Glory: Spanish Town, Jamaica, 1534-2000 (Philip D. Morgan Philippe R. Girard; Paradise Lost: Haiti’s Tumultuous Journey from Pearl of the Caribbean to Third World Hotspot (Carolle Charles Michael Deibert; Notes from the Last Testament: The Struggle for Haiti (Carolle Charles Ellen de Vries; Suriname na de binnenlandse oorlog (Aspha E. Bijnaar In: New West Indian Guide/ Nieuwe West-Indische Gids no. 82 (2008, no: 1-2, Leiden

  2. Integrating top-down and bottom-up approaches to design a cost-effective and equitable programme of measures for adaptation of a river basin to global change

    Science.gov (United States)

    Girard, Corentin; Rinaudo, Jean-Daniel; Pulido-Velazquez, Manuel

    2016-04-01

    the implementation of cooperative game theory to define cost allocation scenarios. These results are compared with cost allocation rules based on social justice principles to provide contrasted insights into a negotiation process. The interdisciplinary framework developed in this research combines economics and water resources engineering methods, establishing a promising means of bridging the gap between bottom-up and top-down approaches and supporting the creation of cost-effective and equitable adaptation plans at the local level. The approach has been applied to the Orb river basin in Southern France. Acknowledgements The study has been partially supported by the IMPADAPT project /CGL2013-48424-C2-1-R) from the Spanish ministry MINECO (Ministerio de Economía y Competitividad) and European FEDER funds. Corentin Girard is supported by a grant from the University Lecturer Training Program (FPU12/03803) of the Ministry of Education, Culture and Sports of Spain.

  3. Towards an equitable allocation of the cost of a global change adaptation plan at the river basin scale: going beyond the perfect cooperation assumption

    Science.gov (United States)

    Girard, Corentin; Rinaudo, Jean-Daniel; Pulido-Velázquez, Manuel

    2015-04-01

    of the adaptation plan, different allocation scenarios are considered. The LCRBOM allows defining a solution space based on economic rationality concepts from cooperative game theory (the core of the game), and then, to define equitable allocation of the cost of the programme of measures (the Shapley value and the nucleolus). Moreover, alternative allocation scenarios have been considered based on axiomatic principles of social justice, such as "utilitarian", "prior rights" or "strict equality", applied in the case study area. The comparison of the cost allocation scenarios brings insight to inform the decision making process at the river basin scale and potentially reap the efficiency gains from cooperation in the design of adaptation plan. The study has been partially supported by the IMPADAPT project /CGL2013-48424-C2-1-R) from the Spanish ministry MINECO (Ministerio de Economía y Competitividad) and European FEDER funds. Corentin Girard is supported by a grant from the University Lecturer Training Program (FPU12/03803) of the Ministry of Education, Culture and Sports of Spain.

  4. Bound states and critical behavior of the Yukawa potential

    Institute of Scientific and Technical Information of China (English)

    LI; Yongyao

    2006-01-01

    [1]Yukawa,H.,On the interaction of elementary particles,Proc.Phys.Math Soc.Jap.,1935,17:48-57.[2]Sachs,R.,Goeppert-Mayer,M.,Calculations on a new neutron-proton interaction potential,Phys.Rev.,1938,53:991-993.[3]Harris,G.,Attractive two-body interactions in partially ionized plasmas,Phys.Rev.,1962,125:1131-1140.[4]Schey,H.,Schwartz,J.,Counting the bound states in short-range central potentials,Phys.Rev.B,1965,139:1428-1432.[5]Rogers,J.,Graboske,H.,Harwood,E.,Bound eigenstates of the static screened Coulomb poten-tial,Phys.Rev.A,1970,1:1577-1586.[6]McEnnan,J.,Kissel,L.,Pratt,R.,Analytic perturbation theory for screened Coulomb potentials:non-relativistic case,Phys.Rev.A,1976,13:532-559.[7]Gerry,C.,Estimates of the ground states of the Yukawa potential from the Bogoliubov inequality,J.Phys.A,1984,17:L313-L315.[8]Kr(o)ger,H.,Girard,R.,Dufour,G.,Direct calculation of the S matrix in coordinate space,Phys.Rev.C,1988,37:486-496.[9]Girard,R.,Kr(o)ger,H.,Labelle,P.et al.,Computation of a long time evolution in a Schr(o)dinger system,Phys.Rev.A,1988,37:3195-3200.[10]Garavelli,S.,Oliveira,F.,Analytical solution for a Yukawa-type potential,Phys.Rev.Lett.,1991,66:1310-1313.[11]Gomes,O.,Chacham,H.,Mohallem,J.,Variational calculations for the bound-unbound transition of the Yukawa potential,Phys.Rev.A,1994,50:228-231.[12]Yukalov,V.,Yukalova,E.,Oliveira,F.,Renormalization-group solutions for Yukawa potential,J.Phys.A,1998,31:4337-4348.[13]Brau,F.,Necessary and sufficient conditions for existence of bound states in a central potential,J.Phys.A,2003,36:9907-9913.[14]Bertini,L.,Mella,M.,Bressanini,D.et al.,Borromean binding in H-2 with Yukawa potential:a nonadiabatic quantum Monte Carlo study,Phys.Rev.A,2004,69:042504.[15]Dean,D.,Drummond,I.,Horgan,R.,Effective diffusion constant in a two-dimensional medium of charged point scatterers,J.Phys.A,2004,37:2039-2046.[16]De-Leo,S.,Rotelli,P.,Amplification of coupling for Yukawa potentials,Phys.Rev.D,2004,69:034006.[17]Khrapak

  5. Ichthyofauna and its community diversity in volcanic barrier lakes of Northeast China%东北地区火山堰塞湖鱼类区系与群落多样性

    Institute of Scientific and Technical Information of China (English)

    杨富亿; 吕宪国; 娄彦景; 娄晓楠; 薛滨; 姚书春; 肖海丰

    2012-01-01

    根据2008-2011年镜泊湖和五大连池鱼类学调查结果和历史资料,分析了东北地区火山堰塞湖鱼类区系特征和群落物种多样性状况.东北地区火山堰塞湖鱼类区系由9目16科47属64种土著种构成,其中国家Ⅱ级野生保护物种1种,中国特有种4种,中国易危种5种;64种鱼类中,鲤形目44种,鲤科37种,分别占优势.东北地区火山堰塞湖鱼类区系由7个区系复合体构成,以东部平原区系复合体为主体;南北方重叠种、北方特有种分别占53.1%和46.9%.东北地区火山堰塞湖鱼类群落Shannon、Fisher-α、Pielou、Margalef和Simpson指数值分别为2.078、4.536、0.575、3.723和0.269,土著种多度分布格局可以用对数正态分布描述;与镜泊湖亚群落间Bray-Curtis、Morisita-Horn、Ochiai、S(o)rensen和Whittaker指数值分别为0.820、0.992、0.870、0.862和0.138,与五大连池亚群落间各指数值分别为0.210、0.516、0.838、0.825和0.175.东北地区火山堰塞湖鱼类区系具有南北方物种相互渗透、古北界与东洋界交汇过渡的特点;群落物种多样性程度较高,物种结构较稳定,但物种丰富度呈下降趋势.%Based on the investigations of fish resources in Jingpo Lake and Wudalianchi Lakes in 2008-2011 and the historical data, this paper analyzed the characteristics of ichthyofauna and its community diversity in volcanic barrier lakes of Northeast China. The ichthyofauna in the volcanic barrier lakes of Northeast China was consisted of 64 native species, belonging to 47 genera, 16 families , and 9 orders, among which, one species was the second class National protected wild animal, four species were Chinese endemic species, and five species were Chinese vulnerable species. In the 64 recorded species, there were 44 species of Cypriniformes order and 37 species of Cyprinidae family dominated, respectively. The ichthyofauna in the volcanic barrier lakes of Northeast China was formed by 7 fauna

  6. 鄱阳湖子湖“堑秋湖”渔业资源结构特征分析%STURCTURE AND FEATURES OF FISHERY RESOURCES OF SUB-LAKES IN POYANG LAKE, JIANGXI, CHINA

    Institute of Scientific and Technical Information of China (English)

    曾泽国; 张笑辰; 刘观华; 金杰峰; 陈家宽; 金斌松

    2015-01-01

    genera,7 of them were commercial fishes,and there was no fisheries production in Shahu because of the low water level.The results of the investigation study showed that the largest group was from Cypriniformes,and had the similar fish species in different years,but the dominant species were changed and the yield of catch had a downward trend.The analysis of non-metrimultidimensional scaling (MDS) showed that community diversity varied among years and sub-lakes.The correlation analysis indicated that there was a positive correlation between CPUE a day and the water level in the year of 2011,while such correlation declined in the year of 2012 and 2013.%“堑秋湖”是广泛存在于鄱阳湖湖区且与鄱阳湖水文节律特征相顺应的一种渔业方式.为了解“堑秋湖”渔获物组成特征及其产量与水位之间的关系,于2011~2013年“堑秋湖”期间对江西鄱阳湖国家级自然保护区的子湖大湖池和沙湖进行了连续3年的渔业调查.2011年,大湖池和沙湖与修河分别相通23 d和10 d,鄱阳湖属旱涝急转气候;2012年鄱阳湖水势较大,大湖池和沙湖与修河分别相通124 d和108 d.2013年鄱阳湖再现旱情,大湖池和沙湖均未能与修河连通.2011年渔业调查开展7次,2012年调查9次,2013年调查5次.3 a调查累计获得鱼类59种隶属于6目12科43属.3年研究结果表明渔获物以鲤科鱼类为主,在种类上变化不大但优势物种有所改变且渔获物产量呈下降趋势,无度量多维排序结果(MSD)显示鱼类群落在不同年度和子湖间分离明显.经济鱼类的单位捕捞努力量(CPUE)与水位变化的线性回归分析结果表明,2011年经济鱼类产量与当日水位呈正相关性,但2012年和2013年两者的相关性却不明显.

  7. 基于生态修复的青海湖沙柳河鱼道建设与维护%Construction and management of fish passage on Shaliu River adjacent to Qinghai Lake based on ecological restoration

    Institute of Scientific and Technical Information of China (English)

    吴晓春; 史建全

    2014-01-01

    Gymnocypris przewalskii belongs to subfamily of Schizothoracinae, family of Cyprinidae and order of Cypriniformes, and it is an endemic species only distributed in the Qinghai Lake and its adjacent rivers and lakes. It possesses migration characteristics, and goes upstream from the lake to surrounding rivers in the reproduction season. However, several dams built on the rivers flowing into the lake with the agricultural development in the Qinghai Lake region since 1970s, severely blocked its migration passages, leading to obviously decreasing of resources of Gymnocypris przewalskii in recent years. To protect Gymnocypris przewalskii, three kinds of fish passages on the dams of rivers flowing into the Qinghai Lake were introduced, which were types of guide wall, simple steel modular and ladder slope, respectively. 1) The guide wall type fish passage was separately constructed on the dams in the Shaliu River, Quanji River and Haergai River in 2006, which were built along river side, but it had several defects because of the lack of experience. 2) The simple steel modular type fish passage was designed and constructed on the dam of the Shaliu River in 2008, Based on biological characteristics of Gymnocypris przewalskii and the basic situation of the dam and environmental conditions of the Shaliu River. After considering inlet location of fish passage, suction flow and hydraulic conditions within passage, the inlet was located at the area with aggregated fish and slow current velocity. The water flow was 0.5 m/s, the current velocity was 0.1-0.25 m3/s, as well as the slope of 1:10 within fish passage. 3) The ladder slope type fish passage was built on the dam of Shaliu River in 2010, which used two types open fishponds of multi-ladder and slight slope. Fish passage without hindrance had been almost come to be real through this type. This type was designed according to basic theories and related parameters of simple fish passage. The length was 80 m and the width was 1

  8. Clinopyroxene application in petrogenesis identification of volcanic rocks associated with salt domes from Shurab (Southeast Qom

    Directory of Open Access Journals (Sweden)

    Somayeh Falahaty

    2016-07-01

    Isfahan for its financial supports. Reference Adams, G.E. and Bishop, F.C., 1986. The olivine- clinopyroxene geobar- ometer: experimental results in the CaO- FeO- MgO- SiO2 system. Contributions to Mineralogy and Petrology, 94(2: 230-237. Droop, G.T.R., 1987. A general equation for estimating Fe3+ in ferromagnesian silicates and oxides from microprobe analysis, using stoichiometric criteria. Mineralogical Magazine, 51(361: 431-437. Helz, R.T., 1973. Phase relations of basalts in their melting range at PH2O= 5 kb as a function of oxygen fugacity. Journal of Petrolology, 17(2: 139-193. Kretz, R., 1994. Metamorphic Crystallization. Chichester and New York, New York, 530 pp. Kushiro, I., 1960. Si- AI relation in clinopyroxenes from igneous rocks. American Journal of Science, 258(5: 548-554. Lebas, N.J., 1962. The role of aluminous in igneous clinopyroxenes with relation to their parentage. American Journal of Science, 260(4: 267-88. Leterrier, J., Maury, R.C., Thonon, P., Girard, D. and Marchal, M., 1982. Clinopyroxene composition as a method of identification of the magmatic affinities of paleo- volcanic series. Earth and Planetary Science Letters, 59(1: 139-154. Morimoto, N., 1988. Nomenclature of pyroxenes. Fortschr mineral, 66: 237-252. Nisbet, E.G. and Pearce, J.A., 1977. Clinopyroxene composition of mafic lavas from different tectonic settings. Contributions to Mineralogy and Petrology, 63(2: 161-173. Schweitzer, E.L., Papike, J.J. and bence, A. E., 1979. Statitical analysis of clinopyroxenes from deep sea basalts. American Mineralogist, 642: 501-513. Soesoo, A., 1997. A multivariate statistical analysis of clinopyroxene composition: empirical coordinates for the crystallisation PT-estimations. Geological Society of Sweden (Geologiska Föreningen, 119(1: 55-60. Verhooge, J., 1962. Distribution of titanium between silicates and oxydes in igneous rocks. American Journal of Science, 260(2: 211-220.

  9. A Massive Parallel Variational Multiscale FEM Scheme Applied to Nonhydrostatic Atmospheric Dynamics

    Science.gov (United States)

    Vazquez, Mariano; Marras, Simone; Moragues, Margarida; Jorba, Oriol; Houzeaux, Guillaume; Aubry, Romain

    2010-05-01

    , 1992-1999 [5] N. Ahmad and J. Lindeman (2007), "Euler solutions using flux-based wave decomposition" Int. J. Numer. Meth. Fluids 54, 47-72 [6] A. Robert (1993), "Bubble convection experiments with a semi-implicit formulation of the Euler equations" J. Atmosph. Sci. 50, 1865-1873 [7] R. B. Smith (2002), "Stratified flow over topography" In Environmental stratified flows, Kluwer Academic Publishers, Ed. Grimshaw R., 119-159 [8] L. Bonaventura (2000), "A semi-implicit, semi-Lagrangian scheme using the height coordinate for a nonhydrostatic and fully elastic model of atmospheric flows" J. Comp. Phys. 158, 186-213 [9] G. J. Mayr and A. Gohm (2000), 2D airflow over a double bell-shaped mountain Meteorol. Atmos. Phys. 72, 13-27 [10] C. Schär and D. Leuenberger and O. Fuhrer and D. Luthic and C. Girard (2002), "A new terrain-following vertical coordinate formulation for atmospheric prediction models" Mon. Weather Rev. 130, 2459-2480

  10. EDITORIAL: Focus on Plasmonics FOCUS ON PLASMONICS

    Science.gov (United States)

    Bozhevolnyi, Sergey; García-Vidal, Francisco

    2008-10-01

    Reuben M Bakker, Vladimir P Drachev, Zhengtong Liu, Hsiao-Kuan Yuan, Rasmus H Pedersen, Alexandra Boltasseva, Jiji Chen, Joseph Irudayaraj, Alexander V Kildishev and Vladimir M Shalaev Confinement and propagation characteristics of subwavelength plasmonic modes R F Oulton, G Bartal, D F P Pile and X Zhang Theory on the scattering of light and surface plasmon polaritons by arrays of holes and dimples in a metal film F de León-Pérez, G Brucoli, F J García-Vidal and L Martín-Moreno Shaping and manipulation of light fields with bottom-up plasmonic structures C Girard, E Dujardin, G Baffou and R Quidant Gold nanorods and nanospheroids for enhancing spontaneous emission A Mohammadi, V Sandoghdar and M Agio Generation of surface plasmons at single subwavelength slits: from slit to ridge plasmon J-Y Laluet, A Drezet, C Genet and T W Ebbesen Mode mapping of plasmonic stars using TPL microscopy P Ghenuche, S Cherukulappurath and R Quidant Controlling optical transmission through magneto-plasmonic crystals with an external magnetic field G A Wurtz, W Hendren, R Pollard, R Atkinson, L Le Guyader, A Kirilyuk, Th Rasing, I I Smolyaninov and A V Zayats Nanoplasmonic renormalization and enhancement of Coulomb interactions M Durach, A Rusina, V I Klimov and M I Stockman Bulk and surface sensitivities of surface plasmon waveguides Pierre Berini Mapping plasmons in nanoantennas via cathodoluminescence R Gómez-Medina, N Yamamoto, M Nakano and F J García de Abajo Theoretical analysis of gold nano-strip gap plasmon resonators T Søndergaard, J Jung, S I Bozhevolnyi and G Della Valle Surface plasmon polariton-mediated enhancement of the emission of dye molecules on metallic gratings J Gómez Rivas, G Vecchi and V Giannini Nanoshells to nanoeggs to nanocups: optical properties of reduced symmetry core shell nanoparticles beyond the quasistatic limit Mark W Knight and Naomi J Halas Single emitters coupled to plasmonic nano-antennas: angular emission and collection efficiency T H Taminiau

  11. Putative relationships among inseminating and externally fertilizing characids, with a description of a new genus and species of Brazilian inseminating fish bearing an anal-fin gland in males (Characiformes: Characidae

    Directory of Open Access Journals (Sweden)

    Stanley H. Weitzman

    2005-09-01

    Bryconadenos and Attonitus are discussed in relation to certain other inseminating characids, such as the species of Brittanichthys and Hollandichthys, that are not currently hypothesized to belong to Clade A and presumably acquired insemination independently. It is concluded that much additional data regarding the reproductive modes as well as other anatomical/physiological systems of characids currently included in and excluded from Clade A are necessary before a reasonably supported phylogeny of Clade A characids and their possible outgroup relatives can be advanced. The anal-fin gland cells of sexually active male Bryconadenos specimens are histologically indistinguishable from club cells (also called alarm substance cells found within the skin of cypriniforms, characiforms, catfishes, and other otophysan fishes. These cells occur at the skin's surface of the anal-fin gland in male Bryconadenos where they are organized into an organ. Many other adult male characids have club cells at the anal-fin's skin surface, often associated with anal-fin hooks, but were not found organized into an organ as in Bryconadenos. We hypothesize these cells to secrete a pheromone during courtship via holocrine secretion. Males of the genera Lophiobrycon , Glandulocauda, and Mimagoniates, tribe Glandulocaudini, were found to have club cells associated with their caudal-fin organ, but no specialized mucus cells were present as found in the caudal organ of males of the glandulocaudine Corynopoma riisei, tribe Stevardiini (= Corynopomini of past authors. In this species, males have hypertrophied mucus cells hypothesized to be modified for pheromone secretion. Evidence that the derived scales and fin rays of the caudal organ of males of the tribe Glandulocaudini are not homologous with that of other tribes of the Glandulocaudinae, as this subfamily was previously recognized, is discussed and it is concluded that the members of the tribe Glandulocaudini should be recognized as a separate subfamily

  12. Frequency, Causes, and Findings of Brain CT Scans of Neonatal Seizure at Besat Hospital, Hamadan, Iran

    Directory of Open Access Journals (Sweden)

    Fateme EGHBALIAN*

    2015-01-01

    . Brain Res Dev Brain Res 2004; 152:137-42.Cornejo BJ, Mesches MH, Coultrap S, Browning MD, Benke TA. A single episode of neonatal seizures permanently alters glutamatergic synapses. Ann Neurol 2007; 61:411-26.Sanchez RM, Dai W, Levada RE, Lippman JJ, Jensen FE. AMPA/kainate receptor-mediated downregulation of GABAergic synaptic transmission by calcineurin after seizures in the developing rat brain. J Neurosci 2005; 25:3442-51.Huang L, Cilio MR, Silveira DC, et al. Long-term effects of neonatal seizures: a behavioral, electrophysiological, and histological study. Brain Res Dev Brain Res 1999; 118:99-107.Sayin U, Sutula TP, Stafstrom CE. Seizures in the developing brain cause adverse long-term effects on spatial learning and anxiety. Epilepsia 2004; 45:1539-48.Fanaroff AA, Martin RJ. Neonatal prenatal medicine. 7th ed. New York: Mosby. 2002.P.887-899.Bluvstein E, Judith B, Moshe B, Soloman N. Current management in child neurology. 2nd ed. Lernard Maria. 2002. P.123-128.Scafer B, Bodensteiner JB. Radiological findings in development delay. Semin Pediatr Neurol 1998 Mar: 37-8.Barkovick AJ. Pediatric neuroimaging. 3rd ed. New York: Lippincott, Williams & Wilkins, 2000: 162-205.Volpe A. Neurology of the newborn. 4th ed. Philadelphia: W.B Saunders. 2000. P.172-202.Swaiman KF, Ashwal S. Pediatric neurology. Principles and practice. 3rd ed. New York: Mosby. 1999.P. 183-188.Haaga JR, Lanzieri CF. Computed tomography and magnetic resonance imaging of the whole body. Third ed. New York: Mosby, 1994.Girard N, Raybaud C. Neonates with Seizures: What to Consider, How to Image. Magnetic Resonance Imaging Clinics of North America 2011 Nov;19(4; 685-708.Christopher P. Hess, A. James Barkovich. Seizures: Emergency Neuroimaging. Neuroimaging Clinics of North America 2010 Nov;20(4:619-637.Memon S, Memon MH. Spectrum and immediate outcome of seizures in neonates. JCPSP 2006; 16(11:717-20.Denizmen A, Filizy Y. Our cases of seizures in neonatal unit. J Pediatr 1997; 6(1:33-6.Park W, Kim DY