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Sample records for alfalfa variety development

  1. Stem formation at alfalfa varieties and correlative dependences with some main parameters

    Directory of Open Access Journals (Sweden)

    Natalya Georgieva

    2015-06-01

    Full Text Available During the period 2006-2009 in IFC-Pleven were studied 9 alfalfa varieties: Europe, Prista 2, Prista 3, Prista 4, Obnova 10, Pleven 6, Dara, Multifoliate and Dama. In the first year of its development alfalfa formed on average 1.42 number of stems per plant. During second and third year their quantity increased to 2.14 and 3.83 number whereafter during fourth year they decreased to 3.28. In all years (except the first year was found a trend of increase in stem number from spring to autumn regrowth with average values 2.11, 2.85 and 3.05 respectively. Average for the four-year period the greatest number of stems (over the average for the nine studied varieties – 2.67 formed the Multifoliate variety followed by Europe, Prista 4, Obnova 10 and Dama. The varieties which are characterized by less density of stand (number of plants per unit area had a greater number of stems per plant (r = -0.530. Correlations with mean and high positive value were found between stem formation and amount and distributions of rainfall during vegetation period (r = 0.989, year of alfalfa development (r = 0.861, nodulation (r = 0.763, weight of root mass (r = 0.411 as well as correlations with mean negative value between number of stems and percentage of damaged stems by Apion seniculus (r = -0.456.

  2. Prohexadione-calcium responsive alfalfa varieties ensure success of corn-interseeded alfalfa production systems

    Science.gov (United States)

    Recent USDA-NASS data indicate alfalfa and corn were planted on about 0.8 and 1.9 million hectares per year, respectively, in the Northeast, Great Lakes, Upper Midwest, and Northern Mountain regions the USA. Because both crops are often grown in rotation, alfalfa could be interseeded at corn plantin...

  3. Alfalfa

    Science.gov (United States)

    Alfalfa is an herb. People use the leaves, sprouts, and seeds to make medicine. Alfalfa is used for kidney conditions, bladder and prostate ... bleeding disorder called thrombocytopenic purpura. People also take alfalfa as a source of vitamins A, C, E, ...

  4. Alfalfa variety selection for maximum fiber content, protein and nitrogen fixation

    Energy Technology Data Exchange (ETDEWEB)

    Ruhland, Christopher T. [Minnesota State Univ., Mankato, MN (United States); Knox, John [Minnesota State Univ., Mankato, MN (United States); Ward, Susan [Minnesota State Univ., Mankato, MN (United States); Agarwal, V. J. [Minnesota State Univ., Mankato, MN (United States); Frey, John [Minnesota State Univ., Mankato, MN (United States); Carrow, Duane [Minnesota State Univ., Mankato, MN (United States); Jones, Bruce [Minnesota State Univ., Mankato, MN (United States); Rife, James [Minnesota State Univ., Mankato, MN (United States)

    2012-09-01

    The fertile soils of Southern Minnesota are highly productive from both a natural and agricultural standpoint. We chose to examine potential feedstock in both of these settings as a focus for our study. In part one of this report we detail our findings examining potential feedstock in prairie and wetlands in the Mankato, MN area in 2009. We were able to familiarize ourselves with techniques used to isolate and quantify concentrations of cellulose, hemicellulose and lignin in both a classroom and laboratory setting. These techniques were then used for our experiments that examined the effects of harvest regime, irrigation and salinity on eight potential-feedstock varieties of alfalfa (Medicago sativa).

  5. Effects of drought stress on germination characters of some alfalfa (Medicago sativa L. varieties

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    Emine BUDAKLI ÇARPICI

    2015-12-01

    Full Text Available This study was conducted to determine the responses of some alfalfa varieties to different drought stress at germination stage. Alfalfa varieties Bilensoy-80, Alsancak, Gözlü-1, Prosementi and Isıde were used as plant materials. Four different drought stress levels (0, -2.95, -4.91 and -10.27 bar were handled in this study and were used Polyethylene glycol-6000 (PEG-6000 to create of drought stress at different levels. The experiment was conducted as completely randomized design with two factors and four replications. In the experiment, some characters such as germination percentage, plumula length, radicle length and vigour index were examined. The results revealed that drought stress had a significant effect on all characters. Germination percentage, plumula length, radicle length, and vigour index ranged 0.00-99.50 %, 0.00-43.24 mm, 0.00-54.45 mm and 0.00-43.79, respectively. Considering germination percentage, the cultivars tolerated the drought stress up to-4.91 bar, but after this level germination percentage decreased significantly. In terms of plumula length and vigour index, the cultivars tolerated the drought stress up to -2.95 bar and in terms of radicle length the cultivars tolerated the drought stress up to -4.91 bar and were observed a significant decreases or deaths after this levels. Bilensoy-80 and Alsancak had been more successful for drought than the other varieties in experiment.

  6. System responses to long-term drought and re-watering of two contrasting alfalfa varieties.

    Science.gov (United States)

    Kang, Yun; Han, Yuanhong; Torres-Jerez, Ivone; Wang, Mingyi; Tang, Yuhong; Monteros, Maria; Udvardi, Michael

    2011-12-01

    Systems analysis of two alfalfa varieties, Wisfal (Medicago sativa ssp. falcata var. Wisfal) and Chilean (M. sativa ssp. sativa var. Chilean), with contrasting tolerance/sensitivity to drought revealed common and divergent responses to drought stress. At a qualitative level, molecular, biochemical, and physiological responses to drought stress were similar in the two varieties, indicating that they employ the same strategies to cope with drought. However, quantitative differences in responses at all levels were revealed that may contribute to greater drought tolerance in Wisfal. These included lower stomatal density and conductance in Wisfal; delayed leaf senescence compared with Chilean; greater root growth following a drought episode, and greater accumulation of osmolytes, including raffinose and galactinol, and flavonoid antioxidants in roots and/or shoots of Wisfal. Genes encoding transcription factors and other regulatory proteins, and genes involved in the biosynthesis of osmolytes and (iso)flavonoids were differentially regulated between the two varieties and represent potential targets for improving drought tolerance in alfalfa in the future.

  7. Comparative physiological and transcriptional analyses of two contrasting drought tolerant alfalfa varieties

    Directory of Open Access Journals (Sweden)

    Wenli eQuan

    2016-01-01

    Full Text Available Drought is one of major environmental determinants of plant growth and productivity. Alfalfa (Medicago sativa is a legume perennial forage crop native to the arid and semi-arid environment, which is an ideal candidate to study the biochemical and molecular mechanisms conferring drought resistance in plants. In this study, drought stress responses of two alfalfa varieties, Longdong and Algonquin, were comparatively assayed at the physiological, morphological and transcriptional levels. Under control condition, the drought-tolerant Longdong with smaller leaf size and lower stomata density showed less water loss than the drought-sensitive Algonquin. After exposing to drought stress, Longdong showed less severe cell membrane damage, more proline and ascorbate (ASC contents and less accumulation of H2O2 than Algonquin. Moreover, significantly higher antioxidant enzymes activities after drought treatment were found in Longdong when compared with Algonquin. In addition, transcriptional expression analysis showed that Longdong exhibited significantly higher transcripts of drought-responsive genes in leaf and root under drought stress condition. Taken together, these results indicated that Longdong variety was more drought-tolerant than Algonquin variety as evidenced by less leaf firing, more lateral root number, higher relative aboveground/underground biomass per plant and survival rate.

  8. Assessment of heat shock protein 70 induction by heat in alfalfa varieties and constitutive overexpression in transgenic plants.

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    Nicoletta Ferradini

    Full Text Available Heat shock proteins (HSPs are molecular chaperones involved in many cellular functions. It has been shown that mammalian cytosolic HSP70 binds antigenic peptides mediating the activation of the immune system, and that it plays a determining role in tumour immunogenicity. This suggests that HSP70 may be used for the production of conjugated vaccines. Human and plant HSPs share high sequence similarity and some important biological functions in vitro. In addition, plant HSPs have no endotoxic side effects. Extraction of HSP70 from plants for use as vaccine adjuvant requires enhancing its concentration in plant tissues. In this work, we explored the possibility to produce HSP70 in both transgenic and non-transgenic plants, using alfalfa as a model species. First, a transcriptional analysis of a constitutive and an inducible HSP70 genes was conducted in Arabidopsis thaliana. Then the coding sequence of the inducible form was cloned and introduced into alfalfa by Agrobacterium-mediated transformation, and the accumulation of the protein in leaf tissue of transgenic plants was demonstrated. We also tested diverse alfalfa varieties for heat-inducible expression of endogenous HSP70, revealing variety-specific responses to heat shock.

  9. Developing PYTHON Codes for the Undergraduate ALFALFA Team

    Science.gov (United States)

    Troischt, Parker; Ryan, Nicholas; Alfalfa Team

    2016-03-01

    We describe here progress toward developing a number of new PYTHON routines to be used by members of the Undergraduate ALFALFA Team. The codes are designed to analyze HI spectra and assist in identifying and categorizing some of the intriguing sources found in the initial blind ALFALFA survey. Numerical integration is performed on extragalactic sources using 21cm line spectra produced with the L-Band Wide receiver at the National Astronomy and Ionosphere Center. Prior to the integration, polynomial fits are employed to obtain an appropriate baseline for each source. The codes developed here are part of a larger team effort to use new PYTHON routines in order to replace, upgrade, or supplement a wealth of existing IDL codes within the collaboration. This work has been supported by NSF Grant AST-1211005.

  10. Effects of alfalfa meal on growth performance and gastrointestinal tract development of growing ducks.

    Science.gov (United States)

    Jiang, J F; Song, X M; Huang, X; Zhou, W D; Wu, J L; Zhu, Z G; Zheng, H C; Jiang, Y Q

    2012-10-01

    A study was conducted to evaluate effects of alfalfa meal on growth performance and gastrointestinal tract development of growing layer ducks to provide evidence for application of alfalfa meal in the duck industry. Two hundred and fifty-six healthy Shaoxing 7-wk old growing layer ducks were selected and randomly allocated to 1 of 4 dietary treatments based on corn and soybean meal and containing 0, 3, 6, and 9% of alfalfa meal for 8 wks. Each treatment consisted of 4 replicates of 16 ducks each. Briefly, birds were raised in separate compartments, and each compartment consisted of three parts: indoor floor house, adjacent open area and a connecting water area. The results showed: i) Growing ducks fed alfalfa meal diet were not significantly different in average daily gain, feed intake and gain-to-feed ratio from those fed no alfalfa diet (p>0.05). ii) Alfalfa meal increased the ratio crop, gizzard to live weight, caecum to live weight, the caecum index of growing ducks (pducks increased significantly with the increase of alfalfa meal levels (pducks decreased significantly with the increase of alfalfa meal levels (pducks could improve gastrointestinal tract growth and small intestinal morphology without effect on performance. This experiment provides evidence that alfalfa meal is a very valuable feedstuff for growing layer ducks.

  11. Alfalfa varieties differ markedly in seedling survival when interseeded into corn and treated with prohexadione-calcium

    Science.gov (United States)

    Interseeded alfalfa could serve as a dual purpose crop for providing groundcover during silage corn production and forage during subsequent years of production, but this system has been unworkable because competition between the co-planted crops often leads to stand failure of interseeded alfalfa. R...

  12. Proteomic Identification of Differentially Expressed Proteins during Alfalfa (Medicago sativa L.) Flower Development.

    Science.gov (United States)

    Chen, Lingling; Chen, Quanzhu; Zhu, Yanqiao; Hou, Longyu; Mao, Peisheng

    2016-01-01

    Flower development, pollination, and fertilization are important stages in the sexual reproduction process of plants; they are also critical steps in the control of seed formation and development. During alfalfa (Medicago sativa L.) seed production, some distinct phenomena such as a low seed setting ratio, serious flower falling, and seed abortion commonly occur. However, the causes of these phenomena are complicated and largely unknown. An understanding of the mechanisms that regulate alfalfa flowering is important in order to increase seed yield. Hence, proteomic technology was used to analyze changes in protein expression during the stages of alfalfa flower development. Flower samples were collected at pre-pollination (S1), pollination (S2), and the post-pollination senescence period (S3). Twenty-four differentially expressed proteins were successfully identified, including 17 down-regulated in pollinated flowers, one up-regulated in pollinated and senesced flowers, and six up-regulated in senesced flowers. The largest proportions of the identified proteins were involved in metabolism, signal transduction, defense response, oxidation reduction, cell death, and programmed cell death (PCD). Their expression profiles demonstrated that energy metabolism, carbohydrate metabolism, and amino acid metabolism provided the nutrient foundation for pollination in alfalfa. Furthermore, there were three proteins involved in multiple metabolic pathways: dual specificity kinase splA-like protein (kinase splALs), carbonic anhydrase, and NADPH: quinone oxidoreductase-like protein. Expression patterns of these proteins indicated that MAPK cascades regulated multiple processes, such as signal transduction, stress response, and cell death. PCD also played an important role in the alfalfa flower developmental process, and regulated both pollination and flower senescence. The current study sheds some light on protein expression profiles during alfalfa flower development and

  13. Proteomic Identification of Differentially Expressed Proteins during Alfalfa (Medicago sativa L.) Flower Development

    Science.gov (United States)

    Chen, Lingling; Chen, Quanzhu; Zhu, Yanqiao; Hou, Longyu; Mao, Peisheng

    2016-01-01

    Flower development, pollination, and fertilization are important stages in the sexual reproduction process of plants; they are also critical steps in the control of seed formation and development. During alfalfa (Medicago sativa L.) seed production, some distinct phenomena such as a low seed setting ratio, serious flower falling, and seed abortion commonly occur. However, the causes of these phenomena are complicated and largely unknown. An understanding of the mechanisms that regulate alfalfa flowering is important in order to increase seed yield. Hence, proteomic technology was used to analyze changes in protein expression during the stages of alfalfa flower development. Flower samples were collected at pre-pollination (S1), pollination (S2), and the post-pollination senescence period (S3). Twenty-four differentially expressed proteins were successfully identified, including 17 down-regulated in pollinated flowers, one up-regulated in pollinated and senesced flowers, and six up-regulated in senesced flowers. The largest proportions of the identified proteins were involved in metabolism, signal transduction, defense response, oxidation reduction, cell death, and programmed cell death (PCD). Their expression profiles demonstrated that energy metabolism, carbohydrate metabolism, and amino acid metabolism provided the nutrient foundation for pollination in alfalfa. Furthermore, there were three proteins involved in multiple metabolic pathways: dual specificity kinase splA-like protein (kinase splALs), carbonic anhydrase, and NADPH: quinone oxidoreductase-like protein. Expression patterns of these proteins indicated that MAPK cascades regulated multiple processes, such as signal transduction, stress response, and cell death. PCD also played an important role in the alfalfa flower developmental process, and regulated both pollination and flower senescence. The current study sheds some light on protein expression profiles during alfalfa flower development and

  14. Proteomic identification of differentially expressed proteins during alfalfa (Medicago sativa L. flower development

    Directory of Open Access Journals (Sweden)

    Lingling Chen

    2016-10-01

    Full Text Available Flower development, pollination, and fertilization are important stages in the sexual reproduction process of plants; they are also critical steps in the control of seed formation and development. During alfalfa (Medicago sativa L. seed production, some distinct phenomena such as a low seed setting ratio, serious flower falling, and seed abortion commonly occur. However, the causes of these phenomena are complicated and largely unknown. An understanding of the mechanisms that regulate alfalfa flowering is important in order to increase seed yield. Hence, proteomic technology was used to analyze changes in protein expression during the stages of alfalfa flower development. Flower samples were collected at pre-pollination (S1, pollination (S2, and the post-pollination senescence period (S3. Twenty-four differentially expressed proteins were successfully identified, including 17 down-regulated in pollinated flowers, one up-regulated in pollinated and senesced flowers, and six up-regulated in senesced flowers. The largest proportions of the identified proteins were involved in metabolism, signal transduction, defense response, oxidation reduction, cell death, and programmed cell death (PCD. Their expression profiles demonstrated that energy metabolism, carbohydrate metabolism, and amino acid metabolism provided the nutrient foundation for pollination in alfalfa. Furthermore, there were three proteins involved in multiple metabolic pathways: dual specificity kinase splA-like protein (kinase splALs, carbonic anhydrase (CA, and NADPH: quinone oxidoreductase-like protein (NQOLs. Expression patterns of these proteins indicated that MAPK cascades regulated multiple processes, such as signal transduction, stress response, and cell death. PCD also played an important role in the alfalfa flower developmental process, and regulated both pollination and flower senescence. The current study sheds some light on protein expression profiles during alfalfa flower

  15. Effects of supplementation level and particle size of alfalfa hay on growth characteristics and rumen development in dairy calves

    NARCIS (Netherlands)

    Mirzaei, M.; Khorvash, M.; Ghorbani, G.R.; Kazemi-Bonchenari, M.; Riasi, A.; Nabipour, A.; Borne, van den J.J.G.C.

    2015-01-01

    The aim of this study was to assess the effects of particle size (PS) of alfalfa hay on growth characteristics and rumen development in dairy calves at two levels of alfalfa supplementation. Fifty newborn dairy calves (42.7 ± 2.2 kg BW) were used in a 2 × 2 factorial arrangement with the factors sup

  16. Glyphosate-tolerant alfalfa is compositionally equivalent to conventional alfalfa (Medicago sativa L.).

    Science.gov (United States)

    McCann, Melinda C; Rogan, Glennon J; Fitzpatrick, Sharie; Trujillo, William A; Sorbet, Roy; Hartnell, Gary F; Riodan, Susan G; Nemeth, Margaret A

    2006-09-20

    Glyphosate-tolerant alfalfa (GTA) was developed to withstand over-the-top applications of glyphosate, the active ingredient in Roundup agricultural herbicides. As a part of the safety assessment, GTA (designated J101 x J163) was grown under controlled field conditions at geographically diverse locations within the United States during the 2001 and 2003 field seasons along with control and other conventional alfalfa varieties for compositional assessment. Field trials were conducted using a randomized complete block design with four replication blocks at each site. Alfalfa forage was harvested at the late bud to early bloom stage from each plot at five field sites in 2001 (establishment year) and from four field sites in 2003 (third year of stand). The concentration of proximate constituents, fibers, amino acids, coumestrol, and minerals in the forage was measured. The results showed that the forage from GTA J101 x J163 is compositionally equivalent to forage from the control and conventional alfalfa varieties.

  17. The Undergraduate ALFALFA Team: A Collaboration for Undergraduate Research Opportunities and Faculty Development

    Science.gov (United States)

    Koopmann, Rebecca A.; Balonek, Thomas J.; Cannon, John M.; Coble, Kimberly A.; Craig, David W.; Durbala, Adriana; Finn, Rose; Hallenbeck, Gregory L.; Haynes, Martha P.; Higdon, Sarah; Hoffman, G. Lyle; Kornreich, David A.; Lebron, Mayra E.; Crone-Odekon, Mary; O'Donoghue, Aileen A.; Olowin, Ronald Paul; Pantoja, Carmen; Rosenberg, Jessica L.; Troischt, Parker; Venkatesan, Aparna; Wilcots, Eric M.; ALFALFA Team

    2016-01-01

    Legacy astronomy surveys involve large collaborations over long time periods, making it challenging to involve undergraduates in meaningful projects. Collaborating with faculty at 19 undergraduate-focused institutions across the US and Puerto Rico and with US-NSF funding, the ALFALFA (Arecibo Legacy Fast ALFA) Team has developed the Undergraduate ALFALFA Team, an effective model to promote undergraduate research and faculty development within the ALFALFA HI blind survey project and follow-up programs. This talk will summarize the main components of the program, which include an annual undergraduate workshop at Arecibo Observatory, observing runs at Arecibo, computer infrastructure, summer and academic year research projects, and dissemination at national meetings (e.g., Cannon et al., Collins, Elliott et al, Craig et al., Hansen et al., Johnson et al., Morrison et al., O'Donoghue et al., Smith et al., Sylvia et al., Troischt et al., this meeting). This work has been supported by NSF grants AST-0724918/0902211, AST-075267/0903394, AST-0725380, and AST-1211005.

  18. Interactions of alfalfa hay and sodium propionate on dairy calf performance and rumen development.

    Science.gov (United States)

    Beiranvand, H; Ghorbani, G R; Khorvash, M; Nabipour, A; Dehghan-Banadaky, M; Homayouni, A; Kargar, S

    2014-01-01

    The objective of this experiment was to investigate the effects of different levels of alfalfa hay (AH) and sodium propionate (Pro) added to starter diets of Holstein calves on growth performance, rumen fermentation characteristics, and rumen development. Forty-two male Holstein calves (40±2kg of birth weight) were used in a complete randomized design with a 3×2 factorial arrangement of treatments. Dietary treatments were as follows: (1) control = concentrate only; (2) Pro = concentrate with 5% sodium propionate [dry matter (DM) basis]; (3) 5% AH = concentrate + 5% alfalfa hay (DM basis); (4) 5% AH + Pro = concentrate + 5% alfalfa hay + 5% sodium propionate (DM basis); (5) 10% AH = concentrate + 10% alfalfa hay (DM basis); and (6) 10% AH + Pro = concentrate + 10% alfalfa hay + 5% sodium propionate (DM basis). All calves were housed in individual pens bedded with sawdust until 10wk of age. They were given ad libitum access to water and starter throughout the experiment and were fed 2L of milk twice daily. Dry matter intake was recorded daily and body weight weekly. Calves from the control, 10% AH, and 10% AH + Pro treatments were euthanized after wk 10, and rumen wall samples were collected. Feeding of forage was found to increase overall dry matter intake, average daily gain, and final weight; supplementing sodium propionate had no effect on these parameters. Calves consuming forage had lower feed efficiency than those on the Pro diet. Rumen fluid in calves consuming forage had higher pH and greater concentrations of total volatile fatty acids and molar acetate. Morphometric parameters of the rumen wall substantiated the effect of AH supplementation, as plaque formation decreased macroscopically. Overall, the interaction between forage and sodium propionate did not affect calf performance parameters measured at the end of the experiment. Furthermore, inclusion of AH in starter diets positively enhanced the growth performance of male Holstein calves and influenced

  19. Urgency of further developing alfalfa industry in China%我国苜蓿产业亟待振兴

    Institute of Scientific and Technical Information of China (English)

    孙启忠; 玉柱; 徐春城

    2012-01-01

    随着我国畜牧业的快速发展,特别是奶牛业的崛起,对优质饲草尤其是苜蓿(Medicago sativa)的需求量将会越来越大。目前我国苜蓿产量低、品质差、供应量不足,不能满足市场的需求.进口苜蓿呈增加态势。2010年苜蓿的进口量已达22.72万t,对我国的苜蓿产业造成严重威胁。我国苜蓿产业发展受制于许多因素,一直处于徘徊不前状态,急需振兴。应尽快制定苜蓿产业发展战略规划,完善苜蓿生产补贴政策,鼓励科技创新,积极扶持苜蓿龙头企业。整合苜蓿产业资源,建设现代化苜蓿产业示范基地,推进我国苜蓿布局区域化、种植规模化、作业机械化、管理标准化和经营产业化发展,着力打造苜蓿产业带,增强我国苜蓿的综合生产能力和供应能力。推进奶一草一体化。%The rapid development of animal husbandry in China, especially the prosperous development of dairy farming, greatly increases the need for herbage of high quality, particularly alfalfa. This study pointed out that the alfalfa yield was low, and its quality was poor, and the supply of alfalfa did not meet its market demand in China. Consequently, the total quantity of imported alfalfa showed a increasing trend and came to 227 200 t in 2010, threatening the development of alfalfa industry in China. This study pro- posed that it is urgent to develop the alfalfa industry because alfalfa industry was regulated by many fac- tors. Finally, this study proposed some suggestions for further developing the alfalfa industry. Firstly, it was urgent to make development plan of alfalfa industry; secondly, the compensation package needed to be improved for alfalfa producer; thirdly, it was effort to innovate the utilization technology and support the leading enterprise of alfalfa industry; fourthly, establishing the demonstration base with modern technology was necessary by integrating the alfalfa

  20. 6种杂草对紫花苜蓿的化感作用%A Study of Allelopathy on Alfalfa with Six Variety of Weeds

    Institute of Scientific and Technical Information of China (English)

    王毅民

    2012-01-01

    This is a study of two alfalfa(Medicago sativa) seeds on seed germination and shoots growing by using water and ethanol extract from six kinds of weeds.The results show that Descuminia Sophia,Ex Prantl,Carduusnutans Linn,Chorispora tenella DC,Capsella bursa-pastoris,Trigonotis peduncularis,and Artemisia frigida Willd.Sp.Pl.all have different degrees of allelopathic effects on alfalfa.Water extracts from CarduusnutansLinn and Chorispora tenella(Pall.) DC,could significantly reduce stem lenghth,dry weight and seed germination rate of Longdong alfalfa and AErGangJin alfalfa.Allelopathy of weeds on AErGangJin alfalfa is greater than that on Longdong alfalfa.%以6种杂草水提液、醇提液对两种紫花苜蓿Medicago sativa品种种子萌发及幼苗生长的影响进行了研究.结果显示:播娘蒿Descuminia sophia(L.)webb.Ex Prantl、飞廉CarduusnutansLinn、离子草Chorisporatenella(Pall.)DC.、荠菜Capsella bursa-pastoris、附地菜Trigonotis peduncularis(Trev.)Benth.和冷蒿Artemis-ia frigida Willd.Sp.Pl.对紫花苜蓿均表现出不同程度的化感作用.飞廉和离子草的水提液显著降低了陇东苜蓿Longdong sativa和阿尔冈金Algonquin幼苗苗长、干质量以及种子的萌发率,播娘蒿的醇提液显著降低了阿尔冈金幼苗苗长、干物质以及种子的萌发率.阿尔冈金品种受杂草的化感作用的影响大于陇东苜蓿品种.

  1. Alfalfa Enod12 genes are differentially regulated during nodule development by Nod factors and Rhizobium invasion.

    Science.gov (United States)

    Bauer, P; Crespi, M D; Szécsi, J; Allison, L A; Schultze, M; Ratet, P; Kondorosi, E; Kondorosi, A

    1994-01-01

    MsEnod12A and MsEnod12B are two early nodulin genes from alfalfa (Medicago sativa). Differential expression of these genes was demonstrated using a reverse transcription-polymerase chain reaction approach. MsEnod12A RNA was detected only in nodules and not in other plant tissues. In contrast, MsEnod12B transcripts were found in nodules and also at low levels in roots, flowers, stems, and leaves. MsEnod12B expression was enhanced in the root early after inoculation with the microsymbiont Rhizobium meliloti and after treatment with purified Nod factors, whereas MsEnod12A induction was detected only when developing nodules were visible. In situ hybridization showed that in nodules, MsEnod12 expression occurred in the infection zone. In empty Fix- nodules the MsEnod12A transcript level was much reduced, and in spontaneous nodules it was not detectable. These data indicate that MsEnod12B expression in roots is related to the action of Nod factors, whereas MsEnod12A expression is associated with the invasion process in nodules. Therefore, alfalfa possesses different mechanisms regulating MsEnod12A and MsEnod12B expression. PMID:8066132

  2. Research on the development of weed-suppressing rice varieties

    Institute of Scientific and Technical Information of China (English)

    Shaochuan ZHOU; Decheng LU; Hong LI; Daoqiang HUANG; Ruowei MIAO

    2009-01-01

    This study was conducted to develop weed-suppressing rice varieties in a weed-suppressing nursery by specific secondary metabolite markers of allelopathic potential. Weed-suppressing rice variety Feng-Hua-Zhan was screened out by a specific secondary metabolite marker of allelopathic potential. Weed-suppressing rice No. 1, a new rice line with good quality, was also developed using this method. The genetic backgrounds and research directions of weed-suppressing rice varieties are discussed in this paper.

  3. Effect of Space Flight Factors on Plant Biomass Developed from Alfalfa Seeds Carried by the Satellite%卫星搭载对紫花苜蓿当代植株生物量的影响

    Institute of Scientific and Technical Information of China (English)

    任卫波; 赵亮; 王蜜; 陈立波; 郭慧琴

    2008-01-01

    [Objective] The aim of this study was to investigate the effect of space flight factors on plant biomass in the generation of alfalfa carried by the satellite. [Method] Seeds from three lines of alfalfa were carried by the seed-breeding satellite Shijian-8. After the satellite returned to the ground, stem diameter, primary branch number and current-year individual biomass of alfalfa were studied. [Result] After space flight, primary branch number and current-year individual biomass of alfalfa increased significantly, while the stem diameter had no significant change. Using the value over(the mean value of control + three standard deviation) as a criterion to screen, the variants with enlarged stem diameter, increased primary branch number and individual biomass was two, five and twelve respectively. [Conclusion] The obtained variants can be used in the variety improvement of alfalfa and its new variety breeding, but whether its favorable variation can inherit stably to the progenies needs further study.

  4. A saturated genetic linkage map of autotetraploid alfalfa (Medicago sativa L.) developed using genotyping-by-sequencing is highly syntenous with the Medicago truncatula genome.

    Science.gov (United States)

    Li, Xuehui; Wei, Yanling; Acharya, Ananta; Jiang, Qingzhen; Kang, Junmei; Brummer, E Charles

    2014-08-21

    A genetic linkage map is a valuable tool for quantitative trait locus mapping, map-based gene cloning, comparative mapping, and whole-genome assembly. Alfalfa, one of the most important forage crops in the world, is autotetraploid, allogamous, and highly heterozygous, characteristics that have impeded the construction of a high-density linkage map using traditional genetic marker systems. Using genotyping-by-sequencing (GBS), we constructed low-cost, reasonably high-density linkage maps for both maternal and paternal parental genomes of an autotetraploid alfalfa F1 population. The resulting maps contain 3591 single-nucleotide polymorphism markers on 64 linkage groups across both parents, with an average density of one marker per 1.5 and 1.0 cM for the maternal and paternal haplotype maps, respectively. Chromosome assignments were made based on homology of markers to the M. truncatula genome. Four linkage groups representing the four haplotypes of each alfalfa chromosome were assigned to each of the eight Medicago chromosomes in both the maternal and paternal parents. The alfalfa linkage groups were highly syntenous with M. truncatula, and clearly identified the known translocation between Chromosomes 4 and 8. In addition, a small inversion on Chromosome 1 was identified between M. truncatula and M. sativa. GBS enabled us to develop a saturated linkage map for alfalfa that greatly improved genome coverage relative to previous maps and that will facilitate investigation of genome structure. GBS could be used in breeding populations to accelerate molecular breeding in alfalfa.

  5. Development of simple sequence repeat markers and diversity analysis in alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Wang, Zan; Yan, Hongwei; Fu, Xinnian; Li, Xuehui; Gao, Hongwen

    2013-04-01

    Efficient and robust molecular markers are essential for molecular breeding in plant. Compared to dominant and bi-allelic markers, multiple alleles of simple sequence repeat (SSR) markers are particularly informative and superior in genetic linkage map and QTL mapping in autotetraploid species like alfalfa. The objective of this study was to enrich SSR markers directly from alfalfa expressed sequence tags (ESTs). A total of 12,371 alfalfa ESTs were retrieved from the National Center for Biotechnology Information. Total 774 SSR-containing ESTs were identified from 716 ESTs. On average, one SSR was found per 7.7 kb of EST sequences. Tri-nucleotide repeats (48.8 %) was the most abundant motif type, followed by di-(26.1 %), tetra-(11.5 %), penta-(9.7 %), and hexanucleotide (3.9 %). One hundred EST-SSR primer pairs were successfully designed and 29 exhibited polymorphism among 28 alfalfa accessions. The allele number per marker ranged from two to 21 with an average of 6.8. The PIC values ranged from 0.195 to 0.896 with an average of 0.608, indicating a high level of polymorphism of the EST-SSR markers. Based on the 29 EST-SSR markers, assessment of genetic diversity was conducted and found that Medicago sativa ssp. sativa was clearly different from the other subspecies. The high transferability of those EST-SSR markers was also found for relative species.

  6. Variation of directional reflectance factors with structural changes of a developing alfalfa canopy

    Science.gov (United States)

    Kirchner, J. A.; Kimes, D. S.; Mcmurtrey, J. E., III

    1982-01-01

    Directional reflectance factors of an alfalfa canopy were determined and related to canopy structure, agronomic variables, and irradiance conditions at four periods during a cutting cycle. Nadir and off-nadir reflectance factors decreased with increasing biomass in Thematic Mapper band 3(0.63-0.69 micrometer) and increased with increasing biomass in band 4(0.76-0.90 micrometer). The sensor view angle had less impact on perceived reflectance as the alfalfa progressed from an erectophile canopy of stems after harvest to a near planophile canopy of leaves at maturity. Studies of directional reflectance are needed for testing and upgrading vegetation canopy models and to aid in the complex interpretation problems presented by aircraft scanners and pointable satellites where illumination and viewing geometries may vary widely. Distinct changes in the patterns of radiance observed by a sensor as structural and biomass changes occur are keys to monitoring the growth and condition of crops.

  7. The Current Status, Problems, and Prospects of Alfalfa (Medicago sativa L. Breeding in China

    Directory of Open Access Journals (Sweden)

    Shangli Shi

    2017-01-01

    Full Text Available This paper reviews the current status, methodology, achievements, and prospects of alfalfa (Medicago sativa L. breeding in China. There are 77 cultivars that have been registered in the country, these include 36 cultivars bred through breeding programs, 17 introduced from overseas, 5 domesticated from wild ecotypes, and 19 through regional collection/breeding programs. Cultivars have been selected for cold resistance, disease resistance, salt tolerance, grazing tolerance, high yield, and early maturity. Most of these cultivars have been developed through conventional breeding techniques, such as selective and cross breeding, and some are now being evaluated that have been developed through the application of transgenic technology. The major problems for alfalfa breeding in China include low success rate, slow progress to breed resistant varieties, lack of breeding materials and their systematic collection, storage and evaluation, lack of advanced breeding techniques, and low adoption rate of new cultivars. There are gaps in alfalfa breeding between China and the developed world. Improvement of alfalfa breeding techniques, development of cultivars with adaptations to different regions within China, and the protection and utilization of alfalfa germplasm resources have been identified as major strategies to improve the efficiency of alfalfa breeding in China.

  8. Genetic Engineering of Alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Wang, Dan; Khurshid, Muhammad; Sun, Zhan Min; Tang, Yi Xiong; Zhou, Mei Liang; Wu, Yan Min

    2016-01-01

    Alfalfa is excellent perennial legume forage for its extensive ecological adaptability, high nutrition value, palatability and biological nitrogen fixation. It plays a very important role in the agriculture, animal husbandry and ecological construction. It is cultivated in all continents. With the development of modern plant breeding and genetic engineering techniques, a large amount of work has been carried out on alfalfa. Here we summarize the recent research advances in genetic engineering of alfalfa breeding, including transformation, quality improvement, stress resistance and as a bioreactor. The review article can enables us to understand the research method, direction and achievements of genetic engineering technology of Alfalfa.

  9. Contrasting pattern of somatic and zygotic embryo development in alfalfa (Medicago sativa L.) as revealed by scanning electron microscopy.

    Science.gov (United States)

    Xu, N; Bewley, J D

    1992-06-01

    Scanning electron microscopy has been used to investigate the morphological changes occurring during the development of alfalfa somatic embryos. Embryos were initiated from callus, transferred to suspension culture and matured on solid agar medium. This developmental pattern was compared to that of zygotic embryos developing in ovulo. Somatic embryos begin as distinct pro-embryos within the callus tissue pieces placed in suspension culture. They become globular and heart-shaped while on solid agar medium and then undergo cotyledon elongation and maturation. Somatic embryos develop comparatively slower at early stages of development and faster at the later stages than zygotic embryos. They lack a well-defined suspensor and have a very rough, poorly-differentiated epidermis, the first layer of which is lost after pro-embryo formation. The cotyledons of somatic embryos are multiple and poorlydeveloped; there appears to be a correlation between the amount of surface roughness of the developing embryo and the extent to which polycotyledony occurs.

  10. 旱作条件下苜蓿品种筛选及保苗技术研究%A Study of Keeping a Full Stand of Seedlings of Various Alfalfa Varieties Under Condition of Dry Farming

    Institute of Scientific and Technical Information of China (English)

    张俊丽; 王川; 刘静

    2012-01-01

    Some high quality and drought-resistant alfalfa varieties suitable to grow in the Middle Arid Zone of Ningxia were screened based on the result of climate change and change trend and combining the meteorological data. An exploration was made on the cultivation technologies such as soil preparation and keeping a full stand of seedlings. An drought-resistant and keeping a full stand of seedlings technology characterized by simplicity and low cost was determined. It is of the opinion that under condition of dry farming (high temperature and drought) the rate of emergence when it was interplanted with buckwheat was higher than that of plastic film mulching and bare land planting and is a simple and easy method for keeping a full stand of seedlings%结合气象资料,根据气候变化的结果与变化趋势,筛选出适合中部干旱带种植的优质抗旱苜蓿品种,探索在干旱条件下整地、保苗等栽培技术措施,确定技术简单、成本低的抗旱保苗技术。认为:旱作条件下(高温干旱),混播荞麦比地膜覆盖、裸地种植的出苗率高,是保苗简单易行的方法。

  11. Estimation of the genetic diversity in tetraploid alfalfa populations based on RAPD markers for breeding purposes.

    Science.gov (United States)

    Nagl, Nevena; Taski-Ajdukovic, Ksenija; Barac, Goran; Baburski, Aleksandar; Seccareccia, Ivana; Milic, Dragan; Katic, Slobodan

    2011-01-01

    Alfalfa is an autotetraploid, allogamous and heterozygous forage legume, whose varieties are synthetic populations. Due to the complex nature of the species, information about genetic diversity of germplasm used in any alfalfa breeding program is most beneficial. The genetic diversity of five alfalfa varieties, involved in progeny tests at Institute of Field and Vegetable Crops, was characterized based on RAPD markers. A total of 60 primers were screened, out of which 17 were selected for the analysis of genetic diversity. A total of 156 polymorphic bands were generated, with 10.6 bands per primer. Number and percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon's information index were used to estimate genetic variation. Variety Zuzana had the highest values for all tested parameters, exhibiting the highest level of variation, whereas variety RSI 20 exhibited the lowest. Analysis of molecular variance (AMOVA) showed that 88.39% of the total genetic variation was attributed to intra-varietal variance. The cluster analysis for individual samples and varieties revealed differences in their population structures: variety Zuzana showed a very high level of genetic variation, Banat and Ghareh were divided in subpopulations, while Pecy and RSI 20 were relatively uniform. Ways of exploiting the investigated germplasm in the breeding programs are suggested in this paper, depending on their population structure and diversity. The RAPD analysis shows potential to be applied in analysis of parental populations in semi-hybrid alfalfa breeding program in both, development of new homogenous germplasm, and identification of promising, complementary germplasm.

  12. Comparative drought response in eleven diverse alfalfa accessions

    Science.gov (United States)

    Alfalfa (Medicago sativa L.) production is often negatively affected by drought stress. This is particularly true for alfalfa that is cultivated on rangeland. Thus, the development of drought-tolerant alfalfa cultivars is of great significance. A greenhouse study was conducted to evaluate 11 alfa...

  13. Effect of synthetic auxin herbicides on seed development and viability in genetically-engineered glyphosate-resistant alfalfa

    Science.gov (United States)

    Feral populations of cultivated crops have the potential to function as bridges and reservoirs that contribute to the unwanted movement of novel genetically engineered (GE) traits. Recognizing that feral alfalfa has the potential to lower genetic purity in alfalfa seed production fields when it is g...

  14. Alfalfa: bioenergy and more

    Science.gov (United States)

    Alfalfa (Medicago sativa) has the potential to be a significant contributor to America's renewable energy future. In an alfalfa biomass energy production system, alfalfa forage would be separated into stem and leave fractions. The stems would be processed to produce energy, and the leaves would be s...

  15. Contrasting Storage Protein Synthesis and Messenger RNA Accumulation during Development of Zygotic and Somatic Embryos of Alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Krochko, J E; Pramanik, S K; Bewley, J D

    1992-05-01

    During development on hormone-free media, somatic embryos pass through distinct morphological stages that superficially resemble those of zygotic embryo development (globular, heart, torpedo, cotyledonary stages). Despite these similarities, they differ from zygotic embryos in the extent of cotyledonary development and the patterns of synthesis and quantitative expression of seed-specific storage proteins (7S, 11S, and 2S proteins). Alfin (7S) is the first storage protein synthesized in developing zygotic embryos (stage IV). The 11S (medicagin) and 2S (Low Molecular Weight, LMW) storage proteins are not detectable until the following stage of development (stage V), although all three are present before the completion of embryo enlargement. Likewise, the 7S storage protein is the first to be synthesized in developing somatic embryos (day 5). Medicagin is evident by day 7 and the LMW protein by day 10. In contrast to zygotic embryos, alfin remains the predominant storage protein in somatic embryos throughout development. Not only are the relative amounts of medicagin and the LMW protein reduced in somatic embryos but the LMW protein is accumulated much later than the other proteins. Quantification of the storage protein mRNAs (7S, 11S, and 2S) by northern blot analysis confirms that there are substantial differences in the patterns of message accumulation in zygotic and somatic embryos of alfalfa (Medicago sativa). In zygotic embryos, the 7S, 11S, and 2S storage protein mRNAs are abundant during maturation and, in particular, during the stages of maximum protein synthesis (alfin, stages VI and VII; medicagin, stage VII; LMW, stage VII). In somatic embryos, the predominance of the 7S storage protein is correlated with increased accumulation of its mRNA, whereas the limited synthesis of the 11S storage protein is associated with much lower steady-state levels of its message. The mRNA for the LMW protein is present already by 3 days after transfer to hormone-free media

  16. Applied Genetics and Genomics in Alfalfa Breeding

    Directory of Open Access Journals (Sweden)

    E. Charles Brummer

    2012-03-01

    Full Text Available Alfalfa (Medicago sativa L., a perennial and outcrossing species, is a widely planted forage legume for hay, pasture and silage throughout the world. Currently, alfalfa breeding relies on recurrent phenotypic selection, but alternatives incorporating molecular marker assisted breeding could enhance genetic gain per unit time and per unit cost, and accelerate alfalfa improvement. Many major quantitative trait loci (QTL related to agronomic traits have been identified by family-based QTL mapping, but in relatively large genomic regions. Candidate genes elucidated from model species have helped to identify some potential causal loci in alfalfa mapping and breeding population for specific traits. Recently, high throughput sequencing technologies, coupled with advanced bioinformatics tools, have been used to identify large numbers of single nucleotide polymorphisms (SNP in alfalfa, which are being developed into markers. These markers will facilitate fine mapping of quantitative traits and genome wide association mapping of agronomic traits and further advanced breeding strategies for alfalfa, such as marker-assisted selection and genomic selection. Based on ideas from the literature, we suggest several ways to improve selection in alfalfa including (1 diversity selection and paternity testing, (2 introgression of QTL and (3 genomic selection.

  17. Alfalfa non-feed uses

    Science.gov (United States)

    Non-feed uses for alfalfa such as biomass energy and phytoremediation could increase alfalfa acreage and improve farm profitability. The new bio-energy alfalfa and production system increased forage yield and ethanol production. New alfalfas with enhanced nitrogen cycling capacities would protect wa...

  18. Expression of nodule-specific genes in alfalfa root nodules blocked at an early stage of development.

    NARCIS (Netherlands)

    Dickstein, R.; Bisseling, T.; Reinhold, V.N.; Ausubel, F.M.

    1988-01-01

    To help dissect the molecular basis of the Rhizobium-legume symbiosis, we used in vitro translation and Northern blot analysis of nodule RNA to examine alfalfa-specific genes (nodulins) expressed in two types of developmentally defective root nodules elicited by Rhizobium meliloti. Fix- nodules were

  19. Implications of season and management protocol on the landscape of gene regulation during diapause development in the Alfalfa Leaf Cutting Bee

    Science.gov (United States)

    : The alfalfa leaf cutting bee, Megachile rotundata, is the world’s most intensively managed solitary bee for commercial pollination. It is the primary pollinator of seed alfalfa, a valuable crop for dairy cow feed. Overwintering bees emerge in the spring during alfalfa bloom to mate an...

  20. Alfalfa (Medicago Sativa L As A Promising Forage In Indonesia

    Directory of Open Access Journals (Sweden)

    Sajimin

    2011-06-01

    Full Text Available Alfalfa (Medicago sativa L or Lucerne is a perennial herbaceous legume with superior forage quality. It is the most important forage crop in the world and it was the first domesticated forage crop. Alfalfa is able to fix nitrogen from the air through a symbiotic relationship with Rhizobium bacteria with N production 7.85 – 10.37 g/m2. Its rooting system can reach 4.5 m that allows it to escape drought. Forage production can reach 15.48 tons of dry matter per ha/year and containing 18.0 – 29.1 % crude protein. Plants can live 3 to 12 years depending on climatic conditions and crop varieties. However, alfalfa is not a tropical plant, thus it has not been widely cultivated in Indonesia. The problem of alfalfa cultivation are high pest attacks and competition with weeds. Therefore, alfalfa cultivation requires attention and good management to obtain optimum yield.

  1. Alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Fu, Chunxiang; Hernandez, Timothy; Zhou, Chuanen; Wang, Zeng-Yu

    2015-01-01

    Alfalfa (Medicago sativa L.) is a high-quality forage crop widely grown throughout the world. This chapter describes an efficient protocol that allows for the generation of large number of transgenic alfalfa plants by sonication-assisted Agrobacterium-mediated transformation. Binary vectors carrying different selectable marker genes that confer resistance to phosphinothricin (bar), kanamycin (npt II), or hygromycin (hph) were used to generate transgenic alfalfa plants. Intact trifoliates collected from clonally propagated plants in the greenhouse were sterilized with bleach and then inoculated with Agrobacterium strain EHA105. More than 80 % of infected leaf pieces could produce rooted transgenic plants in 4-5 months after Agrobacterium-mediated transformation.

  2. Estimation of the Genetic Diversity in Tetraploid Alfalfa Populations Based on RAPD Markers for Breeding Purposes

    Directory of Open Access Journals (Sweden)

    Slobodan Katic

    2011-08-01

    Full Text Available Alfalfa is an autotetraploid, allogamous and heterozygous forage legume, whose varieties are synthetic populations. Due to the complex nature of the species, information about genetic diversity of germplasm used in any alfalfa breeding program is most beneficial. The genetic diversity of five alfalfa varieties, involved in progeny tests at Institute of Field and Vegetable Crops, was characterized based on RAPD markers. A total of 60 primers were screened, out of which 17 were selected for the analysis of genetic diversity. A total of 156 polymorphic bands were generated, with 10.6 bands per primer. Number and percentage of polymorphic loci, effective number of alleles, expected heterozygosity and Shannon’s information index were used to estimate genetic variation. Variety Zuzana had the highest values for all tested parameters, exhibiting the highest level of variation, whereas variety RSI 20 exhibited the lowest. Analysis of molecular variance (AMOVA showed that 88.39% of the total genetic variation was attributed to intra-varietal variance. The cluster analysis for individual samples and varieties revealed differences in their population structures: variety Zuzana showed a very high level of genetic variation, Banat and Ghareh were divided in subpopulations, while Pecy and RSI 20 were relatively uniform. Ways of exploiting the investigated germplasm in the breeding programs are suggested in this paper, depending on their population structure and diversity. The RAPD analysis shows potential to be applied in analysis of parental populations in semi-hybrid alfalfa breeding program in both, development of new homogenous germplasm, and identification of promising, complementary germplasm.

  3. A CLIPS expert system for maximizing alfalfa (Medicago Sativa L.) production

    Science.gov (United States)

    Engel, B. A.; Jones, D. D.; Rhykerd, R. L.; Rhykerd, L. M.; Rhykerd, C. L., Jr.; Rhykerd, C. L.

    1990-01-01

    An alfalfa management expert system originally developed by Purdue University agricultural scientists on the PC Plus expert system shell from Texas Instrument has been updated and successfully converted to CLIPS (C Language Integrated Production System). This reduces the cost and legal restrictions associated with making the expert system available to agribusiness industries, extension personnel and farm managers and operators. The expert system includes recommendations concerning soil drainage, liming, P and K fertilization, weed control, variety selection and seeding rate including pure live seeds.

  4. Evolution of Root Characters of Soybean Varieties Developed in Different Years

    Institute of Scientific and Technical Information of China (English)

    YANG Xiu-hong; WU Zong-pu; ZHANG Guo-dong

    2002-01-01

    It was studied that the evolution of root characteristics among 42 soybean varieties developed in Heilongjiang and Jilin Province in different years. The results showed that there were differences on the root characteristics among soybean varieties. From 1950s to 1990s, root fresh weight, root volume, root surface, root dry weight, lateral root length of main root characters tendedly increased with the variable development years. The root system of the varieties in 1990s was relatively well developed compared with that in the other years. The evolutionary trend of the root system of soybean varieties was increasing in root weight, root volume, root surface and length of lateral root.

  5. Comparación de dos índices cuantitativos de estimación del estado de desarrollo de la alfalfa Comparison of two quantitative indexes for the estimation of alfalfa development stages

    Directory of Open Access Journals (Sweden)

    M.L. Bernáldez

    2006-12-01

    Full Text Available El estado de desarrollo de la alfalfa (Medicago sativa L. es una variable de estudio común en evaluaciones de cultivares, dada su relación con la composición química y la tasa de crecimiento de la pastura. La determinación de los índices cuantitativos "estado medio por conteo" y "estado medio por peso" (EMC y EMP respectivamente permite la descripción del estado de desarrollo en pasturas de alfalfa de una manera objetiva y reproducible. Los índices EMC y EMP describen igualmente el estado de desarrollo de la alfalfa cuando la pastura se encuentra próxima al momento de utilización recomendado en la práctica. La ventaja de estimar EMC en relación a EMP, se basa en la rapidez operativa que ofrece la generación de datos para el cálculo del primero.The developmental stage of alfalfa (Medicago sativa L. is an usual variable of study when evaluating cultivars because of its relationship with chemical composition and pasture growth rate. Determination of quantitative indexes such as "mean stage by count" and "mean stage by weight" (MSC and MSW respectively makes it possible to describe the developmental phenological stages of alfalfa pastures in a more objective and reproducible way. Likewise, both the MSC and MSW indexes, describe the developmental stages of alfalfa when the pasture is close to the recommended utilisation time in practice. The advantage of estimating MSC in relation to MSW is based on the higher operative efficiency offered by the former in data generation for its calculation.

  6. Stress responses in alfalfa (Medicago sativa L.) 12. Sequence analysis of phenylalanine ammonia-lyase (PAL) cDNA clones and appearance of PAL transcripts in elicitor-treated cell cultures and developing plants.

    Science.gov (United States)

    Gowri, G; Paiva, N L; Dixon, R A

    1991-09-01

    An expression library containing cDNAs derived from transcripts from fungal elicitor-treated alfalfa cell suspension cultures was screened with an antiserum raised against phenylalanine ammonia-lyase (PAL) from alfalfa. A single immunoreactive clone was isolated which encoded a full-length PAL cDNA (APAL1) consisting of a 2175 bp open reading frame, 96 bp 5'-untranslated leader and 128 bp 3'-non-coding region. The deduced amino acid sequence was 86.5% similar to that of the PAL2 gene of bean, and encoded a polypeptide of Mr 78,865. A second PAL cDNA species was isolated, whose 3'-untranslated region was 86% identical to that of APAL1. Southern blot analysis indicated that PAL is encoded by a small multigene family in alfalfa. PAL transcript levels were rapidly and massively induced, and preceded increased PAL extractable activity, on exposure of alfalfa suspension cells to elicitor from baker's yeast. PAL transcripts were most abundant in roots, stems and petioles during growth and development of alfalfa seedlings. These studies provide the basis for an examination of the developmental and environmental control of a key enzyme of phenylpropanoid synthesis in a plant species which is readily amenable to stable genetic transformation.

  7. Lexicon development, consumer acceptance, and drivers of liking of quinoa varieties

    Science.gov (United States)

    Quinoa is becoming increasingly popular, with an expanding number of varieties being commercially available. In order to compare the sensory properties of these quinoa varieties, a common sensory lexicon needed to be developed. Thus, the objective of this paper was to develop a lexicon describing ...

  8. Determination of Starch Changes During Growth and Development of Three Plum Varieties

    Directory of Open Access Journals (Sweden)

    Romina Alina Vlaic

    2015-11-01

    Full Text Available The aim of this study is the evaluation of changes which occurred in starch content during the growth and the development of three varieties of plums Stanley, Vânăt de Italia, Tuleu Gras. Depending on variety, the maximum amount of starch was reached in phases 3 and 4; the values have declined as the fruit was approaching the maturity stage. The amount of starch during plum fruit growth and development is influenced by the harvesting phase, variety and the interaction between variety and harvest stage. 

  9. Genotyping-by-sequencing based genome-wide association studies on Verticillium wilt resistance in heterozygous autotetraploid alfalfa (Medicago sativa L.)

    Science.gov (United States)

    Verticillium wilt (VW) is a fungal disease which causes severe yield loss in alfalfa. The most effective method to control the disease is through the development and use of resistant varieties. Identification of gene loci linked to VW resistance will facilitate breeding for the disease-resistant al...

  10. Megasporogenesis and development of the embryo sac in the Palestine variety of Trifolium alexandrinum L.

    Directory of Open Access Journals (Sweden)

    S. Krupko

    2015-01-01

    Full Text Available The structure and development of the embryo sac in Trifolium alexandrinum (Palestine variety, are very similar to that in Trifolium pratense. The embryo sac of Trifolium alexandrinum is monosporal, has eight nuclei and is of the Polygonum type. The Palestine variety of Trifolium alexandrinum can be autogamous.

  11. Lexicon Development, Consumer Acceptance, and Drivers of Liking of Quinoa Varieties.

    Science.gov (United States)

    Wu, Geyang; Ross, Carolyn F; Morris, Craig F; Murphy, Kevin M

    2017-03-07

    Quinoa is becoming increasingly popular, with an expanding number of commercially available varieties. To compare the sensory properties of these quinoa varieties, a common sensory lexicon needs to be developed. Thus, the objective of this study was to develop a lexicon of cooked quinoa and examine consumer acceptance of diverse varieties. A trained panel (n = 9) developed aroma, taste/flavor, texture, and color descriptors to describe the sensory properties of 21 quinoa varieties. In addition, texture of the cooked quinoa was determined using a texture analyzer. Results indicated that the developed lexicon could distinguish among these quinoa varieties, showing significant differences in aromas, taste/flavors, and texture attributes. Specifically, quinoa variety effects were observed for the aromas of caramel, nutty, buttery, grassy, earthy, and woody; taste/flavor of sweet, bitter, grain-like, nutty, earthy, and toasty; and firm, cohesive, pasty, adhesive, crunchy, chewy, astringent, and moist textures. Three varieties, "QQ74," "Linares," and "CO407D," exhibited an adhesive texture that has not been described in other commercialized quinoa. Subsequent consumer evaluation (n = 100) on 6 selected samples found that the "Commercial Red" sample was the most accepted overall whereas the least accepted was the field variety "QQ74." For all consumers, overall acceptance of quinoa was driven by higher intensities of grassy aroma, and firm and crunchy texture. Segmentation of the consumers into 4 groups was explored and showed that consumers varied in their acceptance of specific attributes, particularly texture. From the present study, the quinoa lexicon and key drivers of consumer acceptance can be utilized in the industry to evaluate quinoa varieties, product quality and processing procedures.

  12. The Scheme for Development of Improved Variety Base of Holly in Jiangxia,Wuhan

    Institute of Scientific and Technical Information of China (English)

    LI Wan-de; WU Wen-jing; TAN Jian-wen; ZHOU Ni

    2010-01-01

    This paper briefly introduced the overview of Jiangxia in Wuhan,and the biological characteristics about holly,then preliminary analyzed the necessity and feasibility about the development of improved variety base of holly.It also analyzed the benefits from ecology,society and economy.In addition,it proposed some related suggestion about construction scale,base locations,safeguards and superior tree selections and so on.These measures will provide theoretical reference to the development of improved variety base of holly.

  13. Dynamic Transportation of Endophytic Rhizobia in Plants during Growth Development of Alfalfa%苜蓿生育过程中植株体内内生根瘤菌的运移动态

    Institute of Scientific and Technical Information of China (English)

    张淑卿; 李剑峰; 师尚礼

    2009-01-01

    [Objective] The aim of this study was to investigate the dynamic distribution of endophytic rhizobia in different plant parts during seed development of alfalfa and the carrying mechanism of endophytic rhizobia from alfalfa seeds. [Method] Root, stem, leaf, flower, pod and seed from two varieties of alfalfa in different development stages were used for surface disinfection, and then the present site and quantity change of rhizobia in alfalfa were studied by the dilution-plate method. [Result] The number of rhizobia from underground plants in the same stage was as follows: main root<lateral root<hair root. The number of rhizobia from main root and hair root in different stages was as follows: bearing pod stage>branching stage>florescence, while that from lateral root was as follows: branching stage>florescence>bearing pod stage. The distribution of endophytic rhizobia from aerial plants was as follows: few rhizobia was found in stem during branching stage and bearing pod stage, which accounted for 155 cfu/g and 125 cfu/g respectively. Rhizobia mainly distributed in flower bud during branching stage accounting for 9 400 cfu/g, while only in ovary wall during squaring stage accounting for 18 cfu/5 flowers, and few in pollen (26 cfu/5 flowers), fertilized ovules (26 cfu/50 grains), receptacle (15 cfu/5 flowers) and ovary wall (95 cfu/5 flowers) during florescence. Rhizobia existed in pod peel (3 550 cfu/5 pods) and immature seed (1 135 cfu/50 grains) during bearing pod stage, and greatly distributed in seed coat of mature seeds (1 435 cfu/50 grains), but only few in embryo (32 cfu/50 grains) and cotyledon (22 cfu/50 grains). [Conclusion] There is a significant difference in the distribution of endophytic rhizobia from different parts of aerial alfalfa, which only exists in the parts directly related to seed development.%[目的]了解苜蓿种子形成过程中根瘤菌在植株各部位的动态分布,探明苜蓿种子内生根瘤菌的携带机理.[方

  14. Population Development of Zabrotes subfasciatus (Coleoptera: Chrysomelidae) in Landrace Bean Varieties Occurring in Southwestern Amazonia.

    Science.gov (United States)

    Lopes, L M; Araújo, A E F; Santos, A C V; Santos, V B; Sousa, A H

    2016-02-01

    The common bean, Phaseolus vulgaris (L.), is one of the most important sources of protein worldwide, and Latin America is one of the recognized centers of diversity of this species. However, storage of this product after harvest is not feasible because of bruchid attacks. This study determined the accumulated normalized rate of emergence and the daily emergence rate of Zabrotes subfasciatus (Boheman) (Coleoptera: Chrysomelidae:Bruchinae) in five landrace varieties of common bean (BRL 01, SNA 01, RDR 01, RBC 01, and RBC 13) that occurin southwestern Amazonia. These varieties were selected for this study because they are well-distributed throughout the Amazonian communities. Beans of each variety were infested with 50 unsexed adults, and the insects were removed 13 d after beginning the bioassays. The adult progeny obtained from the feeding substrate were counted and removed every other day after the first emergence, until the end of the emergence period. Differences were observed in the calculated rates of development; however, the time required for development and emergence of the insects was independent. Of the five varieties of bean investigated, we observed that the RDR 01, BRL 01, and SNA 01 cultivars are resistant to Z. subfasciatus; the results indicate that the use of these three varieties can reduce problems associated with bruchid attacks and enable storage of the product after harvesting.

  15. Development and reproduction of Panonychus citri (Prostigmata: Tetranychidae) on different species and varieties of citrus plants.

    Science.gov (United States)

    Zanardi, Odimar Zanuzo; Bordini, Gabriela Pavan; Franco, Aline Aparecida; de Morais, Matheus Rovere; Yamamoto, Pedro Takao

    2015-12-01

    The species and varieties of citrus plants that are currently grown can favor the population growth of the citrus red mite Panonychus citri (McGregor) (Prostigmata: Tetranychidae) and alter the pest management programs in citrus groves. In this study we evaluated, in the laboratory, the development and reproduction of P. citri and estimated its life table parameters when reared on four varieties of Citrus sinensis (L.) Osbeck (Valencia, Pera, Natal, and Hamlin), one variety of Citrus reticulata Blanco (Ponkan) and one variety of Citrus limon (L.) Burm. (Sicilian). The incubation period and egg viability were not affected by the host plant. However, the development and survival of the immature stage were significantly lower on Hamlin orange than on Valencia, Pera and Natal oranges, Ponkan mandarin and Sicilian lemon. The fecundity and oviposition period of females were lower on Hamlin orange than on the other hosts. Mites reared on Valencia orange and Sicilian lemon had a higher net reproductive rate (R 0 ), intrinsic growth rate (r) and finite rate of increase (λ), and a shorter interval between generations (T) than on Pera, Natal and Hamlin oranges and Ponkan mandarin. On the other hand, mites reared on Hamlin orange had the lowest R 0 , r and λ and the highest T among the hosts. Based on the results obtained we recommend that for Valencia orange and Sicilian lemon, the mite monitoring programs should be more intense to detect the initial infestation of pest, avoiding the damage in plants and the increase in production costs.

  16. Analysis of the Development and Countermeasures of Alfalfa Industry in China%中国苜蓿产业发展的现状和面临的问题及对策分析

    Institute of Scientific and Technical Information of China (English)

    李栋

    2012-01-01

    Alfalfa is the king of pasture, it has abundant nutrition, high production and high conversion ratio of protein, is an important coarse fodder of dairy cows. Now there is a shortage of high quality alfalfa in China, domestic demand exceeds supply, the unbalance results in soaring on the prices and the climbing of imports. The main reasons why domestic alfalfa industry develops slowly are shortage of quantity, poof quality, high price, lack of policy supports and matched reaping machines, outmoded processing equipment and the high cost of logistics. To promote our alfalfa industry, our suggestions and countermeasures are definited alfalfa industry strategic position and made a scientific plan. The industry shall be scaled, commercialized, innovate management mechanism and promote industrial development and focus on the research and promotion, enjoy "green pass" policy as well as other agricultural products, and give alfalfa industry special subsidy.%苜蓿营养丰富、产量高、蛋白质转化率高,是奶牛重要的粗饲料.目前中国优质苜蓿资源缺乏,商品苜蓿供不应求,供需失衡造成优质苜蓿价格不断攀升,进口量不断增加.国内苜蓿产业发展缓慢,主要问题是数量少、质量差、价格高;苜蓿种植缺乏政策扶持和配套的收割机械;干草加工设备技术落后,物流成本高.推进国内苜蓿产业发展的对策和建议是明确苜蓿产业战略地位,做好科学规划;产业发展走规模化、企业化模式;创新经营机制,带动产业发展;加大苜蓿产业科研和技术推广;和农产品一样享受“绿色通道”优惠政策;对苜蓿产业实行重点补贴.

  17. Establishing alfalfa in silage corn

    Science.gov (United States)

    According to recent agricultural statistics, alfalfa was planted on 0.44 million acres and harvested from 2.2 million acres and silage corn was planted and harvested from 1.0 million acres per year in Wisconsin. Because both crops are often grown in rotation, alfalfa could be interseeded at corn pla...

  18. Strategies for managing foliar and root rot diseases of alfalfa

    Science.gov (United States)

    Diseases can be a major source of yield loss and stand decline in alfalfa. Surveys were conducted to determine the distribution of pathogens for which there is limited resistance in commercial varieties and tests were done with new crop chemicals to determine their effectiveness in controlling sever...

  19. Effects of untreated and treated wastewater at the morphological, physiological and biochemical levels on seed germination and development of sorghum (Sorghum bicolor (L.) Moench), alfalfa (Medicago sativa L.) and fescue (Festuca arundinacea Schreb.).

    Science.gov (United States)

    Rekik, Imen; Chaabane, Zayneb; Missaoui, Amara; Bouket, Ali Chenari; Luptakova, Lenka; Elleuch, Amine; Belbahri, Lassaad

    2017-03-15

    Wastewater reuse in agriculture may help mitigate water scarcity. This may be reached if high quality treatments removing harmful pollutants are applied. The aim of the present study was to compare the effect of untreated (UTW) and treated wastewater (TW) on germination and seedlings development of alfalfa (Medicago sativa L.), fescue (Festuca arundinacea Schreb.) and sorghum (Sorghum bicolor (L.) Moench). UTW presented high turbidity (130 NTU), chemical and biological oxygen demand (COD, 719mgL(-1), BOD5, 291mgL(-1)) and metal concentrations. These levels caused mortality (18% for fescue), decreased germination speed in seeds (37.5% for alfalfa) and reductions of root and stem length in seedlings (80% and 22% respectively for alfalfa). Adverse effects on seeds germination were reflected at the biochemical level by increased H2O2 levels (6 times for sorghum after 5days) and by increased Malondialdehyde (MDA) levels (more than 600 times for sorghum roots) during seedlings development. When TW was used, these parameters were close to control seeds ones. They were also dependent on plant species and developmental stage. Therefore, for efficient reclaimed wastewater reuse in irrigation, suitable crops, displaying wide tolerance to toxic contents during germination and later seedling development stages have to be selected.

  20. Varieties of Male-Sexual-Identity Development in Clinical Practice:A Neuropsychoanalytic Model

    Directory of Open Access Journals (Sweden)

    Frans eStortelder

    2014-12-01

    Full Text Available Variations of sexual-identity development are present in all cultures, as well as in many animal species. Freud, founding father of psychoanalysis, believed that all men have an inherited, bisexual disposition, and that many varieties of love and desire are experienced as alternative pathways to intimacy.In the neuropsychoanalytic model, psychic development starts with the constitutional self. The constitutional self is comprised of the neurobiological factors which contribute to sexual-identity development. These neurobiological factors are focused on biphasic sexual organization in the prenatal phase, based on variations in genes, sex hormones, and brain circuits. This psychosocial construction of sexual identity is determined through contingent mirroring by the parents and peers of the constitutional self. The development of the self—or personal identity—is linked with the development of sexual identity, gender-role identity, and procreative identity. Incongruent mirroring of the constitutional self causes alienation in the development of the self. Such alienation can be treated within the psychoanalytic relationship.This article presents a contemporary, neuropsychoanalytic, developmental theory of male-sexual identity relating to varieties in male-sexual-identity development, with implications for psychoanalytic treatment, and is illustrated with three vugnettes from clinical practice.

  1. Varieties of male-sexual-identity development in clinical practice: a neuropsychoanalytic model.

    Science.gov (United States)

    Stortelder, Frans

    2014-01-01

    Variations of sexual identity development are present in all cultures, as well as in many animal species. Freud - founding father of psychoanalysis - believed that all men have an inherited, bisexual disposition, and that many varieties of love and desire are experienced as alternative pathways to intimacy. In the neuropsychoanalytic model, psychic development starts with the constitutional self. The constitutional self is comprised of the neurobiological factors which contribute to sexual identity development. These neurobiological factors are focused on biphasic sexual organization in the prenatal phase, based on variations in genes, sex hormones, and brain circuits. This psychosocial construction of sexual identity is determined through contingent mirroring by the parents and peers of the constitutional self. The development of the self-or personal identity-is linked with the development of sexual identity, gender-role identity, and procreative identity. Incongruent mirroring of the constitutional self causes alienation in the development of the self. Such alienation can be treated within the psychoanalytic relationship. This article presents a contemporary, neuropsychoanalytic, developmental theory of male-sexual identity relating to varieties in male-sexual-identity development, with implications for psychoanalytic treatment, and is illustrated with three vignettes from clinical practice.

  2. Revisiting the Economic Injury Level and Economic Threshold Model for Potato Leafhopper (Hemiptera: Cicadellidae) in Alfalfa.

    Science.gov (United States)

    Chasen, Elissa M; Undersander, Dan J; Cullen, Eileen M

    2015-08-01

    The economic injury level for potato leafhopper, Empoasca fabae (Harris), in alfalfa (Medicago sativa L.) was developed over 30 yr ago. In response to increasing market value of alfalfa, farmers and consultants are interested in reducing the economic threshold for potato leafhopper in alfalfa. To address this question, caged field trials were established on two consecutive potato leafhopper susceptible crops in 2013. Field cages were infested with a range of potato leafhopper densities to create a linear regression of alfalfa yield response. The slopes, or yield loss per insect, for the linear regressions of both trials were used to calculate an economic injury level for a range of current alfalfa market values and control costs. This yield-loss relationship is the first quantification that could be used to help assess whether the economic threshold should be lowered, given the increased market value of alfalfa.

  3. Photosynthesis phased response to salt stress in fall dormancy standard varieties of alfalfa%盐胁迫对不同秋眠等级紫花苜蓿光合作用的影响

    Institute of Scientific and Technical Information of China (English)

    陈托兄

    2015-01-01

    用0、50、100、150和200 mM NaCl溶液处理不同秋眠等级紫花苜蓿标准品种Maver-ick、Archer和CUF101,观察形态特征和盐害级别,测定叶片净光合速率(Pn)、细胞间隙CO2浓度( Ci)和气孔导度( Gs),分析盐胁迫对不同秋眠等级紫花苜蓿光合特性的影响。不同秋眠等级紫花苜蓿植株叶片盐害级别随品种和处理浓度不同而不同,但均随NaCl浓度的增加和胁迫时间的延长而加大。在较低NaCl浓度下Maverick、Archer和CUF101光合响应存在着预警阶段-恢复阶段-耗尽阶段,在高NaCl浓度下,所有的供试品种光合响应直接进入耗尽阶段。%The photosynthetic responses of Maverick, Archer and CUF101 to salinity stress by different NaCl concentrations(0, 50, 100, 150, 200 mM) were studied in this project, observing morphological features and levels of salt injury.The net photosynthetic rate(Pn), intercellular CO2 concentration (Ci) and stomatal con-ductance( Gs) were measured using Li-6400 under NaCl stress.with the increase of the NaCl concentration and stress time, the level of salt damage had increased in Maverick, Archer and CUF101, while the level of salt damage was different between the varieties and treatments.The photosynthetic response consisted of alarm phase, phase of resistance or phase of restoration, phase of exhaustion, under 50 and 100 mM NaCl.While the photo-synthetic response had nothing but phase of exhaustion, under 150 and 200 mM NaCl.

  4. [Alfalfa Planting as weed control

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This document is a letter to farming cooperators regarding the stipulations surrounding alfalfa plantings in lieu of small grain plantings to provide weed control,...

  5. Trends in leaf photosynthesis in historical rice varieties developed in the Philippines since 1966.

    Science.gov (United States)

    Hubbart, S; Peng, S; Horton, P; Chen, Y; Murchie, E H

    2007-01-01

    Crop improvement in terms of yield is rarely linked to leaf photosynthesis. However, in certain crop plants such as rice, it is predicted that an increase in photosynthetic rate will be required to support future grain yield potential. In order to understand the relationships between yield improvement and leaf photosynthesis, controlled environment conditions were used to grow 10 varieties which were released from the International Rice Research Institute (IRRI) between 1966 and 1995 and one newly developed line. Two growth light intensities were used: high light (1500 micromol m(-2) s(-1)) and low light (300 micromol m(-2) s(-1)). Gas exchange, leaf protein, chlorophyll, and leaf morphology were measured in the ninth leaf on the main stem. A high level of variation was observed among high light-grown plants for light-saturated photosynthetic rate per unit leaf area (P(max)), stomatal conductance (g), content of ribulose bisphosphate carboxylase-oxygenase (Rubisco), and total leaf protein content. Notably, between 1966 and 1980 there was a decline in P(max), g, leaf protein, chlorophyll, and Rubisco content. Values recovered in those varieties released after 1980. This striking trend coincides with a previous published observation that grain yield in IRRI varieties released prior to 1980 correlated with harvest index whereas that for those released after 1980 correlated with biomass. P(max) showed significant correlations with both g and Rubisco content. Large differences were observed between high light- and low light-grown plants (photoacclimation). The photoacclimation 'range' for P(max) correlated with P(max) in high light-grown plants. It is concluded that (i) leaf photosynthesis may be systematically affected by breeding strategy; (ii) P(max) is a useful target for yield improvements where yield is limited by biomass production rather than partitioning; and (iii) the capacity for photoacclimation is related to high P(max) values.

  6. NUTRITIONAL ENHANCEMENT OF ALFALFA THROUGH GENETIC ENGINEERING

    Directory of Open Access Journals (Sweden)

    J. Faragó

    2008-09-01

    Full Text Available Alfalfa (Medicago sativa L. is a pasture legume crop of primary importance to animal production throughout the world. The nutritional quality of alfalfa, as of other leguminous forage crops, is mainly determined by their content in selected essential amino acids (EAAs, such as methionine (Met and cysteine (Cys. In alfalfa, however, these S-containing amino acids constitute only about 1% or less of crude proteins (Frame et al., 1998. This is significantly less than the 3.5% Met+Cys content in the recommended FAO reference protein (FAO, 1973. Recent advances in genetic engineering allow to use the transgenic approach to increase the content of specific essential amino acids in target plant species. A number of different molecular approaches have been developed to address this issue, such as over-expression of a heterologous or homologous Met-rich protein, expression of a synthetic protein, modification of protein sequence, and metabolic engineering of the free amino acid pool and protein sink. To study the possibility of transgenic enhancement of nutritional quality of alfalfa, we used the approach of expression of a heterologous protein rich in Met+Cys in cells of alfalfa. The T-DNA introduced into the genome of alfalfa, using Agrobacterium tumefaciens-mediated genetic transformation, contained the selectable merker gene nptII for kanamycin (Kn resistance, and a cDNA of Ov gene from Japanese quail (Coturnix coturnix coding for a high Met+Cys containing ovalbumine (Mucha et al., 1991, both under constitutive promoters. After cocultivation of petiole segment- and leaf blade-explants of two highly embryogenic alfalfa genotypes Rg9/I-14-22 and Rg11/I-10-68 (Faragó et al., 1997 with cells of A. tumefaciens strain AGL1 carrying the nptII and Ov genes, and selection of transgenic cells on Kn containing selective media, more than one hundred putatively transgenic regenerants were obtained through somatic embryogenesis. Biological (Kn rooting assay

  7. Development of Isogenic Restorer Line in Extra Long Staple Cotton Variety Suvin

    Directory of Open Access Journals (Sweden)

    S. Manickam, K. N. Gururajan and N. Gopalakrishnan

    2010-07-01

    Full Text Available Cotton is the most important commercial crop of India and heterosis in cotton is well documented with 7 to 50per cent heterosis in interspecific (G. hirsutum x G. barbadense hybrids, 10 to 138 per cent in intra-hirsutum hybrids andupto 220 per cent in desi (G. herbaceum x G. arboreum hybrids. Currently hybrid cotton, especially Bt cotton hybrids,occupies more than 80 per cent of cultivated area under cotton in India. Male sterility system offers tremendousadvantage to produce quality hybrid seeds at affordable price so that even marginal farmers can take up hybridcultivation. In cotton both GMS and CGMS systems are available. In cotton, two types of male sterility viz., Genic(GMS and Cytoplasmic-Genic (CGMS systems have been identified. Both the systems have been utilized for thecommercial hybrid seed production in India and other countries. In CGMS system, the male parent of the hybrid needsto be converted into Restorer line. In cotton, Suvin is the only extra long staple G. barbadense variety available which iscapable of spinning to 120s count yarn. This variety has been successfully converted into Restorer line through backcross breeding utilizing Pima Restorer as the donor parent. Suvin Restorer is found to stably restore fertility in differentCMS lines of G. hirsutum. The interspecific hybrids developed using Suvin Restorer was found to combine both highyield and superior fibre quality capable of spinning upto 80s count and are comparable with conventional check hybrids.

  8. Alfalfa (Medicago sativa L.) shoot saponins: identification and bio-activity by the assessment of aphid feeding.

    Science.gov (United States)

    Mazahery-Laghab, H; Yazdi-Samadi, B; Bagheri, M; Bagheri, A R

    2011-01-01

    Biochemical components in alfalfa (Medicago sativa L.), such as saponins, can act as protecting factors against bio-stresses. Saponins are also antifeedants and show oral toxicity towards higher and lower animals. Changes in saponins, such as variation in the carbon skeleton, or hydrolysis of saponin glycosides and other conjugates, may change their biological effects. The aims of this research were to study saponin variation in different growth stages of alfalfa and to investigate the biological role of saponins in the spotted alfalfa aphid, Therioaphis maculata. Saponins from alfalfa shoots in different growth stages were extracted, chemically purified and analysed by TLC. Specific saponins such as soyasaponin1 from root and shoot and two bisdesmosides of medicagenic acid, one from shoot and another from root tissues, were identified using reference compounds allowing changes in saponin composition during plant development in different shoot tissues of alfalfa to be assessed. The response of the alfalfa aphid to feeding on alfalfa in different growth stages was studied. No significant difference in the survival of aphids, from neonate to adult, was observed, but due to the antibiotic effects of saponins, two differences were found in the onset of nymph production and cumulative nymph production. The results show that the saponin composition in alfalfa changes with plant development and this, in turn, can often negatively affect the development of specific insect pests such as the spotted alfalfa aphid, suggesting a possible biological role of alfalfa saponins.

  9. Selfing rate in an alfalfa seed production field pollinated with leafcutter bees

    Science.gov (United States)

    Self-pollination or “selfing” in autotetraploid alfalfa (Medicago sativa L.) (2n = 4x = 32) leads to severe inbreeding depression. Investigating selfing in alfalfa seed production may allow mitigation strategy development against potential negative impacts of selfing on varietal performance. Using m...

  10. Alfalfa transgene dispersal and adventitious presence: understanding grower perception of risk

    Science.gov (United States)

    Recognizing the importance of coexistence, the alfalfa industry has developed a set of Best Management Practices (BMP) to maintain separation of GE and conventional production. But the success of BMP depends upon the degree that growers comply. Therefore we surveyed 530 alfalfa hay and seed producer...

  11. Genetically engineered alfalfa and feral alfalfa plants: What should growers know?

    Science.gov (United States)

    Alfalfa (Medicago sativa subsp. sativa L) is the world’s most important forage crop. The western United States is the most important production area for both alfalfa forage and alfalfa seed. Alfalfa was the first major perennial genetically-engineered (GE)crop and a GE trait for resistance to glypho...

  12. Caryopsis Development and Main Quality Characteristics in Different indica Rice Varieties

    Institute of Scientific and Technical Information of China (English)

    XIONG Fei; WANG Zhong; CHENG Gang; WANG Jue

    2005-01-01

    A comparison on the caryopsis development, rice quality characteristics and the size, shape, structure of the endosperm amyloplasts (starch granules) between two indica rice varieties was made. The main quality traits in Yangdao 6 were better than those in Xiangzaoxian 33; In the early period after fertilization the dry matter in Yangdao 6 was accumulated more slowly than that in Xiangzaoxian 33 but faster in the later period, and the starch was accumulated strongly in the later period; There were two kinds of amyloplasts: single and compound amyloplasts, being 4.4 μm and 9.5 μm in diameter on average with the range of 2.4-8.0 μm and 5.7-19.5 μm, respectively. In the case of Xiangzaoxian 33, most of the single amyloplasts were elliptic or round with loose arrangement and great difference in size, and the coefficient of variation was high. While in the Yangdao 6, most of the amyloplasts were single, well developed, polyhedral, crystalline and compactly arranged, and the coefficient of variation was low. The amyloplasts in the dorsal region of endosperm were developed better than those in the ventral and central regions. The chalkiness in the endosperm resulted from badly-developed and loose-arranged starch granules, which was closely relevant to the transport of filling materials.

  13. A variety of opportunities for immune interactions during trophoblast development and invasion.

    Science.gov (United States)

    Huppertz, Berthold; Berghold, Veronika M; Kawaguchi, Rie; Gauster, Martin

    2012-05-01

    During human implantation and placentation, the direct cell to cell contact of fetal and maternal tissues gives room for a variety of immune interactions. Especially, the invasion of a subset of fetal trophoblast cells, called extravillous trophoblast, generate a very close interplay between the two individuals, enabling the attachment of the placenta to the uterine wall and the transformation of maternal spiral arteries to facilitate adequate nutrition of the fetus. During pregnancy, maternal and fetal factors closely interact to maintain pregnancy and smooth the process of delivery. At each and every stage and site, immunological interactions take place, including attachment of the blastocyst, development and invasion of trophoblast, and flow of maternal plasma and blood through the intervillous space of the placenta. Control mechanisms tightly regulate these interactions helping to evade fetal rejection by the mother. In this review, we highlight the morphological sites of development and feto-maternal interaction to help immunological interested scientists and clinicians to develop hypotheses on the feto-maternal immunological network during pregnancy.

  14. Some Questions Of The Ideological And Political Variety Influence On The Development Of Civil Society

    Directory of Open Access Journals (Sweden)

    Denis A. Nazarov

    2014-06-01

    Full Text Available In the present article author made an attempt to cover constitutional and legal basics of ideological and political variety and influence of these constitutional principles on the development of the civil society institutes in Russian Federation at the present stage. In the article author proves that process of the front of civil society expansion puts in the forefront questions of the public authorities and municipal governments place and role, which is due to the accumulation of force and energy of many institutes of civil society, first of all, to the governmental institutions and resources and their quality. In the conclusion author explains that historically in Russia during the XVIII-XX centuries relation were based on the objective right. Of course, since then many historical changes occurred, but, perhaps, these distinctions and today are the reason of bad compatibility of methodologies of the West and modern Russia. West seeks to teach modern Russia to do "as we" and in practice of the Russian society the culture of objective and subjective coordination in the management and in law develops only now. In the course of the research author research opinions of domestic scientists-jurists.

  15. Deviant behavior variety scale: development and validation with a sample of Portuguese adolescents

    Directory of Open Access Journals (Sweden)

    Cristina Sanches

    2016-01-01

    Full Text Available Abstract This study presents the development and analysis of the psychometric properties of the Deviant Behavior Variety Scale (DBVS. Participants were 861 Portuguese adolescents (54 % female, aged between 12 and 19 years old. Two alternative models were tested using Confirmatory Factor Analysis. Although both models showed good fit indexes, the two-factor model didn’t presented discriminant validity. Further results provided evidence for the factorial and the convergent validity of the single-factor structure of the DVBS, which has also shown good internal consistency. Criterion validity was evaluated through the association with related variables, such as age and school failure, as well as the scale’s ability to capture group differences, namely between genders and school retentions, and finally by comparing a sub-group of convicted adolescents with a group of non-convicted ones regarding their engagement in delinquent activities. Overall, the scale presented good psychometric properties, with results supporting that the DBVS is a valid and reliable self-reported measure to evaluate adolescents’ involvement in deviance.

  16. AGROCHEMICALS GROWTH REGULATORS ON ALFALFA

    Directory of Open Access Journals (Sweden)

    Buldykova I. A.

    2015-04-01

    Full Text Available One of the methods of increasing the productivity of alfalfa is the use of growth regulators, and the use of polymeric compositions of growth regulators, binders with synthesized growth regulators of sim-triazine series is promising with the possibility of creating environmentally friendly technologies for growing alfalfa. Studies have shown that the application of the test on alfalfa growth regulators has a positive effect on growth, physiological and morphogenetic processes, plant resistance to adverse environmental conditions. Intensity impact on plant growth regulators depends on the type of plant growth regulators, concentration and method of application. Processing alfalfa seed growth regulators on germination increases energy 3,0-14,0 % germination on 8,0-17,0 %. Processing plant growth regulators to enhance the growth of plants in height (on the 7th day – 2,6-11,9 % , on the 14th 41,9-48,0 % , the growth of aboveground biomass , expands on the number of productive branches of the 1st ( 24,1-41,3 % and 2nd order (21,7-55,0 %. Pre-sowing seed treatment and plant growth regulators alfalfa sim- triazine series contributed to the increase in seed yield of 15,5 %. On average, the yield of green mass increased by 0,8-2,4 t /ha or 5,2-15,5 % and seed yield at 0,19-0,42 h/ha or 8,7-19, 3 %. Growth regulators of sim-triazine series increase the protein content of the vegetative mass of alfalfa at 3,2-4,6 %

  17. Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway.

    Science.gov (United States)

    Stolze, Anna; Wanke, Alan; van Deenen, Nicole; Geyer, Roland; Prüfer, Dirk; Schulze Gronover, Christian

    2016-11-25

    Natural rubber (NR) is an important raw material for a large number of industrial products. The primary source of NR is the rubber tree Hevea brasiliensis, but increased worldwide demand means that alternative sustainable sources are urgently required. The Russian dandelion (Taraxacum koksaghyz Rodin) is such an alternative because large amounts of NR are produced in its root system. However, rubber biosynthesis must be improved to develop T. koksaghyz into a commercially feasible crop. In addition to NR, T. koksaghyz also produces large amounts of the reserve carbohydrate inulin, which is stored in parenchymal root cell vacuoles near the phloem, adjacent to apoplastically separated laticifers. In contrast to NR, which accumulates throughout the year even during dormancy, inulin is synthesized during the summer and is degraded from the autumn onwards when root tissues undergo a sink-to-source transition. We carried out a comprehensive analysis of inulin and NR metabolism in T. koksaghyz and its close relative T. brevicorniculatum and functionally characterized the key enzyme fructan 1-exohydrolase (1-FEH), which catalyses the degradation of inulin to fructose and sucrose. The constitutive overexpression of Tk1-FEH almost doubled the rubber content in the roots of two dandelion species without any trade-offs in terms of plant fitness. To our knowledge, this is the first study showing that energy supplied by the reserve carbohydrate inulin can be used to promote the synthesis of NR in dandelions, providing a basis for the breeding of rubber-enriched varieties for industrial rubber production.

  18. Impact of preceding crop on alfalfa competitiveness with weeds

    Science.gov (United States)

    Organic producers would like to include no-till practices in their farming systems. We are seeking to develop a continuous no-till system for organic farming, based on a complex rotation that includes a 3-year sequence of alfalfa. In this study, we evaluated impact of preceding crop on weed infest...

  19. Screening for Salnity Tolerance Among Falcata Alfalfa PI's

    Science.gov (United States)

    Many western US rangelands high in salinity could benefit from a salt tolerant falcata type alfalfa. Our objective was to use a previously developed greenhouse screening protocol to characterize 32 PI's from the NPGS system for their relative ability to survive increasing levels of NaC1 relative to...

  20. Alfalfa stem tissues: Cell wall deposition, composition, and degradability

    NARCIS (Netherlands)

    Jung, H.G.; Engels, F.M.

    2002-01-01

    Declining cell wall degradability of alfalfa (Medicago sativa L.) stems with maturation limits the nutritional value of alfalfa for ruminants. This study characterized changes in cell wall concentration, composition, and degradability by rumen microbes resulting from alfalfa stem tissue proliferatio

  1. Effects of Different Eucalyptus Varieties(Clones) on Fitness and Development of Leptocybe invasa Fisher et La Salle

    Institute of Scientific and Technical Information of China (English)

    Kang; Wentong; Zhang; Huafeng; Chen; Shunli; Wei; Chujiang; Zhang; Silu

    2014-01-01

    The damage status of L. invasa on different Eucalyptus varieties( clones) were surveyed in the field,and effects of Eucalyptus varieties on fitness,development and reproduction of this pest were studied in the study. Results showed that:( 1) Most of the major Eucalyptus varieties( clones) could be attacked by L. invasa in Fujian Province,and galls formed on E. urophylla × E. camaldulensis DH201-2,E. gradis QG3,E. dunnii,E. urophylla cU6,E. grandis × E. urophylla GL9,E. robusta and E. exserta; L. invasa could develop and complete its life cycle on above varieties.( 2) The damage degree of different Eucalyptus varieties( clones) was different from fitness degree to L. invasa. L. invasa had different selection frequency,fecundity and number of larvae per gall on different Eucalyptus varieties( clones).( 3) In addition to little difference in female ratios,L. invasa feeding different Eucalyptus varieties( clones) had extremely significant differences in the indicators such as number of emerged L. invasa,body mass of female adult,fecundity,longevity,etc.( 4) According to the fitness index( FI),the host plants of L. invasa were divided into 4 types by cluster analysis: suitable host,moderately suitable host,less suitable host and unsuitable host; the fecundity of each female was more than 70 eggs in suitable host,which was adequate to lead to potential population outbreak of the pest. Host fitness index could be used as a comprehensive evaluation index for screening of resistant varieties( clones) in production.

  2. [Determination of Hard Rate of Alfalfa (Medicago sativa L.) Seeds with Near Infrared Spectroscopy].

    Science.gov (United States)

    Wang, Xin-xun; Chen, Ling-ling; Zhang, Yun-wei; Mao, Pei-sheng

    2016-03-01

    Alfalfa (Medicago sativa L.) is the most commonly grown forage crop due to its better quality characteristics and high adaptability in China. However, there was 20%-80% hard seeds in alfalfa which could not be identified easily from non hard seeds which would cause the loss of seed utilization value and plant production. This experiment was designed for 121 samples of alfalfa. Seeds were collected according to different regions, harvested year and varieties. 31 samples were artificial matched as hard rates ranging from 20% to 80% to establish a model for hard seed rate by near infrared spectroscopy (NIRS) with Partial Least Square (PLS). The objective of this study was to establish a model and to estimate the efficiency of NIRS for determining hard rate of alfalfa seeds. The results showed that the correlation coefficient (R2(cal)) of calibration model was 0.981 6, root mean square error of cross validation (RMSECV) was 5.32, and the ratio of prediction to deviation (RPD) was 3.58. The forecast model in this experiment presented the satisfied precision. The proposed method using NIRS technology is feasible for identification and classification of hard seed in alfalfa. A new method, as nondestructive testing of hard seed rate, was provided to theoretical basis for fast nondestructive detection of hard seed rates in alfalfa.

  3. Plant development and yield of four sugarcane varieties irrigated by a subsurface drip irrigation system in Campinas, Brazil

    Science.gov (United States)

    Silva, André Luiz Barros de O.; Célia de Matos Pires, Regina; Yukitaka Pessinati Ohashi, Augusto; Vasconcelos Ribeiro, Rafael; Landell, Marcos Guimarães de Andrade; Aparecida Creste Dias de Souza, Silvana

    2013-04-01

    The biofuel production is a growing concern on modern society due to the agricultural sustainability, in which both food and energy supply should be taken into account. The agroclimatic zoning indicates that sugarcane expansion in Brazil can only take place in marginal lands, where water deficit occurs and irrigation is necessary. The use of subsurface drip irrigation (SDI) in sugarcane cultivation is an interesting cultural practice to improve production and allow cultivation in marginal lands due to water deficit conditions or to attain high yield and to increase longevity of plants. In this context it is necessary to investigate responses of different varieties to water supply. The aim of this work was to evaluate the plant development and yield of four sugarcane varieties irrigated by a subsurface drip irrigation system in Campinas, Brazil in the 1st cane ratoon cycle. The field experiment was carried out in Campinas SP Brazil, with IACSP95-5000, IACSP94-2094, IACSP94-2101 and SP79-1011 cultivars in the 1st cane ratoon cycle, from January (after the harvest of cane plant cycle) to October (harvest the 1st cane ratoon cycle). The plant spacing was 1.5 m between rows. Each cultivar was planted in an area of 0.4 hectares. The irrigation was done by a subsuperficial drip system with one drip line in each plant row installed at 0.25 m deep. During the 1st cane ratoon cycle the parameters were analysed on the 33rd, 123rd, 185th and 277th day. The analysed parameters were: plant yield (m), leaf area index (LAI) and yield (tons per hectare). According to the results from the second sampling (123rd day) the varieties IACSP95-5000 and IACSP94-2101 showed higher plant height when compared to the other varieties. However, from the third sampling (185th day) on the IACSP95-5000 variety grew considerably taller than the other varieties. The varieties SP79-1011and IACSP94-2101 presented lower values of LAI throughout the crop cycle when compared to other varieties. But on the

  4. Modelling the dynamics of feral alfalfa populations and its management implications.

    Directory of Open Access Journals (Sweden)

    Muthukumar V Bagavathiannan

    Full Text Available BACKGROUND: Feral populations of cultivated crops can pose challenges to novel trait confinement within agricultural landscapes. Simulation models can be helpful in investigating the underlying dynamics of feral populations and determining suitable management options. METHODOLOGY/PRINCIPAL FINDINGS: We developed a stage-structured matrix population model for roadside feral alfalfa populations occurring in southern Manitoba, Canada. The model accounted for the existence of density-dependence and recruitment subsidy in feral populations. We used the model to investigate the long-term dynamics of feral alfalfa populations, and to evaluate the effectiveness of simulated management strategies such as herbicide application and mowing in controlling feral alfalfa. Results suggest that alfalfa populations occurring in roadside habitats can be persistent and less likely to go extinct under current roadverge management scenarios. Management attempts focused on controlling adult plants alone can be counterproductive due to the presence of density-dependent effects. Targeted herbicide application, which can achieve complete control of seedlings, rosettes and established plants, will be an effective strategy, but the seedbank population may contribute to new recruits. In regions where roadside mowing is regularly practiced, devising a timely mowing strategy (early- to mid-August for southern Manitoba, one that can totally prevent seed production, will be a feasible option for managing feral alfalfa populations. CONCLUSIONS/SIGNIFICANCE: Feral alfalfa populations can be persistent in roadside habitats. Timely mowing or regular targeted herbicide application will be effective in managing feral alfalfa populations and limit feral-population-mediated gene flow in alfalfa. However, in the context of novel trait confinement, the extent to which feral alfalfa populations need to be managed will be dictated by the tolerance levels established by specific production

  5. Hybrid-breeding of medicinally used valerian (Valeriana officinalis L. s.l.. A possible concept developing new varieties?

    Directory of Open Access Journals (Sweden)

    Penzkofer, Michael

    2016-07-01

    Full Text Available The aim of this work was to develop and verify a new concept for breeding new hybrid-varieties of valerian without a male sterility system. For this the cross-pollination rate and the performance of inbreeded plants must be determined.

  6. EARLY DEVELOPMENT OF CASTOR BEANS GROWN UNDER SALINITY CONDITIONS (VARIETIES BRS ENERGIA, MPA 34 AND MPB 01

    Directory of Open Access Journals (Sweden)

    RAFAEL ANTÔNIO PRESOTTO

    2016-01-01

    Full Text Available Studies have shown that some plant species exhibit satisfactory production levels when grown under high salinity levels, whereas others exhibit decreased production due to sodium sensitivity even at low sodium concentrations. The castor bean is moderately sensitive to salinity. The aim of the present study was to evaluate the early growth of three castor bean varieties (BRS Energia, MPA 34 and MPB 01 grown in nutrient solution with increasing sodium concentrations (control, 25, 50, 75 and 100 mmol L - 1 . A completely randomized split - plot experimental design was used, with a 5x3 factorial scheme and three replicates per treatment. Salinity resulted in decreased dry weight of all castor bean varieties grown for 31 days under hydroponic conditions. The dry weight accumulation was less affected at the root than at the shoot level. Nevertheless, the shoot dry weight decreased with the increasing salinity. MPA 34 exhibited higher early growth than the remaining tested varieties. Salinity affected the early development of the tested castor bean varieties, and this effect was more pronounced at the shoot than at the root. Variety MPA 34 is promising for cultivation under moderate salinity levels.

  7. Biomass yield development of early, medium and late Maize varieties under a future climate in Lower Saxony, Germany

    Science.gov (United States)

    Degener, J. F.; Kappas, M.

    2014-06-01

    Lower Saxony, with a total land area of about 46 500 km2, constitutes one of the most important agricultural areas in Germany and thus within Europe. Roughly one third of its agricultural area is used for maize cultivation and as of today only few information exist on how a future changing climate will affect its local growing conditions. Thus the newly developed carbon-based crop model BioSTAR and a high-resolution regional climate data-set (Wettreg) were used to evaluate the change in biomass yields of an early, medium and late maize variety. The climate input data is based on the SRES A1B scenario, with a potential fertilization effect or better still, an increased water use efficiency due to rising CO2 levels, taken into account. The biomass yield for all varieties was calculated for each year from 2001 until 2099 on a total of 91 014 sites. The results suggest clearly differentiated development paths of all varieties. All three show a significant positive trend until the end of the century. However the medium variety shows a statistical significant decline of 5% during the first 30 years and only a slight recovery towards +5% around the century's end. The late variety has the clearest and strongest positive trend, with partially more than 30% increase of biomass yields around the end of the century or +25% mean increase in the last three decades. The early variety can be seen as in-between, with no negative but also not an as strong positive development path. All varieties have their strongest increase in yields after the mid of the 21st century. Statistical evaluation of these results suggests that the shift from a summer rain to a winter rain climate in Germany will be the main limiting factor for all varieties. In addition summer temperatures will become less optimal for all maize crops. Only if the plants can supply themselves sufficiently with water outside of the increasingly dry summer months, when also temperatures are much more favorable, an increase

  8. Biomass yield development of early, medium and late Maize varieties under a future climate in Lower Saxony, Germany

    Directory of Open Access Journals (Sweden)

    J. F. Degener

    2014-06-01

    Full Text Available Lower Saxony, with a total land area of about 46 500 km2, constitutes one of the most important agricultural areas in Germany and thus within Europe. Roughly one third of its agricultural area is used for maize cultivation and as of today only few information exist on how a future changing climate will affect its local growing conditions. Thus the newly developed carbon-based crop model BioSTAR and a high-resolution regional climate data-set (Wettreg were used to evaluate the change in biomass yields of an early, medium and late maize variety. The climate input data is based on the SRES A1B scenario, with a potential fertilization effect or better still, an increased water use efficiency due to rising CO2 levels, taken into account. The biomass yield for all varieties was calculated for each year from 2001 until 2099 on a total of 91 014 sites. The results suggest clearly differentiated development paths of all varieties. All three show a significant positive trend until the end of the century. However the medium variety shows a statistical significant decline of 5% during the first 30 years and only a slight recovery towards +5% around the century's end. The late variety has the clearest and strongest positive trend, with partially more than 30% increase of biomass yields around the end of the century or +25% mean increase in the last three decades. The early variety can be seen as in-between, with no negative but also not an as strong positive development path. All varieties have their strongest increase in yields after the mid of the 21st century. Statistical evaluation of these results suggests that the shift from a summer rain to a winter rain climate in Germany will be the main limiting factor for all varieties. In addition summer temperatures will become less optimal for all maize crops. Only if the plants can supply themselves sufficiently with water outside of the increasingly dry summer months, when also temperatures are much more

  9. Variety of urban tourism development trajectories: Antalya, Amsterdam and Liverpool compared

    NARCIS (Netherlands)

    Erkuş-Öztürk, H.; Terhorst, P.

    2012-01-01

    Savitch and Kantor explain divergent trajectories of urban development with the help of four variables, namely, market conditions, inter-governmental support, local culture and popular control in their theory. In this article, we apply Savitch and Kantor's theory to the urban tourism development of

  10. Characterization of celiac disease related oat proteins: bases for the development of high quality oat varieties suitable for celiac patients

    Science.gov (United States)

    Giménez, María J.; Real, Ana; García-Molina, M. Dolores; Sousa, Carolina; Barro, Francisco

    2017-01-01

    Some studies have suggested that the immunogenicity of oats depends on the cultivar. RP-HPLC has been proposed as a useful technique to select varieties of oats with reduced immunogenicity. The aim of this study was to identify both the avenin protein patterns associated with low gluten content and the available variability for the development of new non-toxic oat cultivars. The peaks of alcohol-soluble avenins of a collection of landraces and cultivars of oats have been characterized based on the RP-HPLC elution times. The immunotoxicity of oat varieties for patients with celiac disease (CD) has been tested using a competitive ELISA based on G12 monoclonal antibody. The oat lines show, on average, seven avenin peaks giving profiles with certain similarities. Based on this similarity, most of the accessions have been grouped into avenin patterns. The variability of RP-HPLC profiles of the collection is great, but not sufficient to uniquely identify the different varieties of the set. Overall, the immunogenicity of the collection is less than 20 ppm. However, there is a different distribution of toxicity ranges between the different peak patterns. We conclude that the RP-HPLC technique is useful to establish groups of varieties differing in degree of toxicity for CD patients. PMID:28209962

  11. Proteome analysis of alfalfa roots in response to water deifcit stress

    Institute of Scientific and Technical Information of China (English)

    Rahman Md Atikur; Kim Yong-Goo; AlamIftekhar; LIU Gong-she; Lee Hyoshin; Lee Jeung Joo; Lee Byung-Hyun

    2016-01-01

    To evaluate the response of alfalfa to water deifcit (WD) stress, WD-induced candidates were investigated through a proteomic approach. Alfalfa seedlings were exposed to WD stress for 12 and 15 days respectively, folowed by 3 days re-watering. Water deifcit increased H2O2content, lipid peroxidation, DPPH (1,1-diphenyl-2-picrylhydrazyl)-radical scavenging activity, and the free proline level in alfalfa roots. Root proteins were extracted and separated by two-dimentional polyacrylamide gel electrophoresis (2-DE). A total of 49 WD-responsive proteins were identiifed in alfalfa roots; 25 proteins were reproducibly found to be up-regulated and 24 were down-regulated. Two proteins, namely cytosolic ascorbate peroxidase (APx2) and putative F-box protein were newly detected on 2-DE maps of WD-treated plants. We identiifed several proteins including agamous-like 65, albumin b-32, inward rectifying potassium channel, and auxin-independent growth promoter. The identiifed proteins are involved in a variety of celular functions including calcium signaling, abacisic acid (ABA) biosynthesis, reactive oxygen species (ROS) regulation, transcription/ translation, antioxidant/detoxiifcation/stress defense, energy metabolism, signal transduction, and storage. These results indicate the potential candidates were responsible for adaptive response in alfalfa roots.

  12. Identification of Alfalfa Leaf Diseases Using Image Recognition Technology

    Science.gov (United States)

    Qin, Feng; Liu, Dongxia; Sun, Bingda; Ruan, Liu; Ma, Zhanhong; Wang, Haiguang

    2016-01-01

    Common leaf spot (caused by Pseudopeziza medicaginis), rust (caused by Uromyces striatus), Leptosphaerulina leaf spot (caused by Leptosphaerulina briosiana) and Cercospora leaf spot (caused by Cercospora medicaginis) are the four common types of alfalfa leaf diseases. Timely and accurate diagnoses of these diseases are critical for disease management, alfalfa quality control and the healthy development of the alfalfa industry. In this study, the identification and diagnosis of the four types of alfalfa leaf diseases were investigated using pattern recognition algorithms based on image-processing technology. A sub-image with one or multiple typical lesions was obtained by artificial cutting from each acquired digital disease image. Then the sub-images were segmented using twelve lesion segmentation methods integrated with clustering algorithms (including K_means clustering, fuzzy C-means clustering and K_median clustering) and supervised classification algorithms (including logistic regression analysis, Naive Bayes algorithm, classification and regression tree, and linear discriminant analysis). After a comprehensive comparison, the segmentation method integrating the K_median clustering algorithm and linear discriminant analysis was chosen to obtain lesion images. After the lesion segmentation using this method, a total of 129 texture, color and shape features were extracted from the lesion images. Based on the features selected using three methods (ReliefF, 1R and correlation-based feature selection), disease recognition models were built using three supervised learning methods, including the random forest, support vector machine (SVM) and K-nearest neighbor methods. A comparison of the recognition results of the models was conducted. The results showed that when the ReliefF method was used for feature selection, the SVM model built with the most important 45 features (selected from a total of 129 features) was the optimal model. For this SVM model, the

  13. Participatory plant breeding and organic agriculture: A synergistic model for organic variety development in the United States

    Directory of Open Access Journals (Sweden)

    Adrienne C. Shelton

    2016-12-01

    Full Text Available Abstract Organic farmers require improved varieties that have been adapted to their unique soils, nutrient inputs, management practices, and pest pressures. One way to develop adapted varieties is to situate breeding programs in the environment of intended use, such as directly on organic farms, and in collaboration with organic farmers. This model is a form of participatory plant breeding, and was originally created in order to meet the needs of under-served, small-scale farmers in developing countries. A robust body of literature supports the quantitative genetic selection theory of participatory plant breeding, and helps to explain its increasing prevalence among organic breeding projects in the United States. The history of the organic farming movement in the United States highlights the cultural relevance of engaging organic farmers in the breeding process, complementing the biological rationale for participatory plant breeding. In addition, limited private investment in organic plant breeding encourages the involvement of plant breeders at public institutions. This paper synthesizes the biological, cultural, and economic justifications for utilizing participatory plant breeding as an appropriate methodology for organic cultivar development.

  14. Peanut Variety Tests

    Science.gov (United States)

    Managed peanut variety trials located in various state-wide regions are an essential part of peanut variety development and release. In this study, trials were conducted in Caddo, Custer, and Tillman counties of Oklahoma. Trial entries included 8 runner types, 4 Spanish types, and 4 Virginia types...

  15. Identification of loci associated with drought resistance traits in heterozygous autotetraploid alfalfa (Medicago sativa L.) using genome-wide association studies with genotyping by sequencing

    Science.gov (United States)

    Developing molecular markers associated with drought resistance would be helpful for improving the accuracy in selecting resistant alfalfa, and accelerating the breeding process. A panel of accessions comprised of 200 alfalfa cultivars and landraces with potential drought tolerance were selected fro...

  16. Research Progress of Alfalfa Silage%苜蓿青贮研究进展

    Institute of Scientific and Technical Information of China (English)

    刘振宇; 玉柱; 智建飞; 谢楠; 秦文利; 冯伟; 刘忠宽

    2013-01-01

    As an important protein feed resources, alfalfa silage is of great significance for alfalfa industry and the healthy development of animal husbandry in China.In this paper, the research progress of development history and present situation of alfalfa silage, fermentation promotation, fermentation inhibiting and mixed silage were summarized, and the current main problems in alfalfa silage industry development and future research directions were put forward, in order to provide theoretical and practical references for the alfalfa silage research.%苜蓿作为一种重要的蛋白饲料资源,苜蓿青贮对我国苜蓿产业和畜牧业的健康发展具有重要意义。对苜蓿青贮发展历史与现状以及发酵促进、发酵抑制及混合青贮等研究进行了概述,提出了当前苜蓿青贮产业发展中存在的主要问题和今后的研究方向,以期为苜蓿青贮研究提供理论依据与实践参考。

  17. Effectivenes of inoculation in alfalfa breeding in ecological conditions of the Bjelovar and Bilogora county

    Directory of Open Access Journals (Sweden)

    Darko Uher

    2012-09-01

    Full Text Available Development and basic existence of animal production as well as production of high quality milk depends upon possibility of sufficient production of quality and protein sufficient forage. Forage crop that satisfies these demands is alfalfa which is one of the most important perennial forage crop legumes. The aim of this study was to enhance alfalfa production on acid soil by liming and alfalfa seed inoculation with efficient Sinorhizobium meliloti strains in order to reduce the use of mineral nitrogen fertilization and enable qualitative and cost effective production of forage on the dairy farms. Field trial was established at family farm in the area of Bjelovar and Bilogora county. During two years experimental period statistically significant influence of inoculation and liming on forage and dry matteryield was determined. Significantly the lowest yields were determined on untreated plots without liming material. In all untreated plots, significantly lower yields were determined, but significant differences in yields were also obtained by inoculation with different S. meliloti strains, emphasizing the importance of strains selection used for alfalfa inoculation. In both experimental years total forage yield were ranging from 34 t/ha (untreated plots without liming up to 60 t/ha on plots inoculated with strain 2011 and without liming. Values of total dry matter yield for both experimental years ranged from 6.5 t/ha (untreated plots without liming up to 15,7 t/ha on plots inoculated with strain 2011 without liming. Results of this study showed that application of liming materials for acidity removal had positive effect on alfalfa yields in both experimental years and significantly improved alfalfa production on acid soils. The results of this study clearly showed that inoculation with selected S. meliloti strains may improve alfalfa production on acid soils and may contribute to more efficient forage production for dairy farms under particular

  18. Alfalfa -- a sustainable crop for biomass energy production

    Science.gov (United States)

    Alfalfa (Medicago sativa) has the potential to be a significant contributor to America's renewable energy future. In an alfalfa biomass energy production system, alfalfa forage would be separated into stem and leave fractions. The stems would be processed to produce energy, and the leaves would be s...

  19. The Role of Proanthocyanidins Complex in Structure and Nutrition Interaction in Alfalfa Forage.

    Science.gov (United States)

    Jonker, Arjan; Yu, Peiqiang

    2016-05-23

    Alfalfa (Medicago sativa L.) is one of the main forages grown in the world. Alfalfa is a winter hardy, drought tolerant, N-fixing legume with a good longevity, high yield, high nutrient levels, high digestibility, unique structural to non-structural components ratio, high dry matter intake, and high animal productivity per hectare. However, its main limitation is its excessively rapid initial rate of protein degradation in the rumen, which results in pasture bloat and inefficient use of protein with consequent excessive excretions of nitrogen into the environment. Proanthocyanidins are secondary plant metabolites that can bind with protein and thereby reduce the rate and extent of ruminal protein degradation. However, these secondary metabolites do not accumulate in alfalfa. This review aims to firstly describe the events involved in the rapid release of protein from alfalfa and its effect on ruminant nutrition, environmental pollution, and pasture bloat; secondly, to describe occurrence, structure, functions and benefits of moderate amounts of proanthocyanidin; and finally, to describe the development of alfalfa which accumulates moderate amounts of proanthocyanidins. The emphasis of this review focuses on the role of proanthocyanidins compounds in structure and nutrition interaction in ruminant livestock systems.

  20. The Role of Proanthocyanidins Complex in Structure and Nutrition Interaction in Alfalfa Forage

    Directory of Open Access Journals (Sweden)

    Arjan Jonker

    2016-05-01

    Full Text Available Alfalfa (Medicago sativa L. is one of the main forages grown in the world. Alfalfa is a winter hardy, drought tolerant, N-fixing legume with a good longevity, high yield, high nutrient levels, high digestibility, unique structural to non-structural components ratio, high dry matter intake, and high animal productivity per hectare. However, its main limitation is its excessively rapid initial rate of protein degradation in the rumen, which results in pasture bloat and inefficient use of protein with consequent excessive excretions of nitrogen into the environment. Proanthocyanidins are secondary plant metabolites that can bind with protein and thereby reduce the rate and extent of ruminal protein degradation. However, these secondary metabolites do not accumulate in alfalfa. This review aims to firstly describe the events involved in the rapid release of protein from alfalfa and its effect on ruminant nutrition, environmental pollution, and pasture bloat; secondly, to describe occurrence, structure, functions and benefits of moderate amounts of proanthocyanidin; and finally, to describe the development of alfalfa which accumulates moderate amounts of proanthocyanidins. The emphasis of this review focuses on the role of proanthocyanidins compounds in structure and nutrition interaction in ruminant livestock systems.

  1. Transgenic alfalfa plants expressing the sweetpotato Orange gene exhibit enhanced abiotic stress tolerance.

    Directory of Open Access Journals (Sweden)

    Zhi Wang

    Full Text Available Alfalfa (Medicago sativa L., a perennial forage crop with high nutritional content, is widely distributed in various environments worldwide. We recently demonstrated that the sweetpotato Orange gene (IbOr is involved in increasing carotenoid accumulation and enhancing resistance to multiple abiotic stresses. In this study, in an effort to improve the nutritional quality and environmental stress tolerance of alfalfa, we transferred the IbOr gene into alfalfa (cv. Xinjiang Daye under the control of an oxidative stress-inducible peroxidase (SWPA2 promoter through Agrobacterium tumefaciens-mediated transformation. Among the 11 transgenic alfalfa lines (referred to as SOR plants, three lines (SOR2, SOR3, and SOR8 selected based on their IbOr transcript levels were examined for their tolerance to methyl viologen (MV-induced oxidative stress in a leaf disc assay. The SOR plants exhibited less damage in response to MV-mediated oxidative stress and salt stress than non-transgenic plants. The SOR plants also exhibited enhanced tolerance to drought stress, along with higher total carotenoid levels. The results suggest that SOR alfalfa plants would be useful as forage crops with improved nutritional value and increased tolerance to multiple abiotic stresses, which would enhance the development of sustainable agriculture on marginal lands.

  2. Optimization of enzyme-assisted extraction of polysaccharides from alfalfa and its antioxidant activity.

    Science.gov (United States)

    Wang, Shaopu; Dong, Xiaofang; Tong, Jianming

    2013-11-01

    In this present study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from alfalfa using four factors at five levels central composite rotatable response surface design (CCRD). The experimental data was fitted to a second order polynomial equation with high coefficient of determination values (R(2)>0.95). The results of statistical analysis showed that the linear and quadratic terms of these four variables had significant effects (Penzyme concentration of 2.5%, 2.0%, 3.0% (weight of alfalfa) of cellulase, papain and pectase, extraction temperature 52.7 °C, extraction pH 3.87, ratio of water to raw material 78.92 mL/g and extraction time 2.73 h. Under the optimal conditions, the experimental extraction yield of alfalfa polysaccharides was 5.05 ± 0.02%, which was well matched with the value (5.09%) predicted by the CCRD model. Moreover, evaluation of the antioxidant activity of polysaccharides from alfalfa in vitro suggested that the polysaccharides had good antioxidant effect, especially scavenging activity for hydroxyl radical and DPPH radical, which indicated that the polysaccharides from alfalfa may be explored as a novel natural antioxidant.

  3. [Simulation on the restoration effect of soil moisture in alfalfa (Medicago sativa)-grain rotation system in semi-arid and drought-prone regions of Loess Plateau].

    Science.gov (United States)

    Wang, Xue-Chun; Li, Jun; Fang, Xin-Yu; Sun, Jian; Tahir, Muhammad Naveed

    2011-01-01

    With the combination of field survey and EPIC modeling, this paper simulated the restoration effect of soil moisture in different alfalfa (Medicago sativa)-grain rotation systems in semi-arid and drought-prone regions of Loess Plateau. In perennial alfalfa field and in grain crop field after alfalfa, the correlation coefficients between the simulated and observed values of soil moisture content in 0-10 m layer were larger than 0.9 (P alfalfa field. During the cultivation of alfalfa, the soil moisture content in the layers at 8-10 m depth should not be less than 5.7%. Considering the sustainable development of agricultural production, the appropriate cultivation duration of alfalfa should be 4-6 years and no more than 8 years. For the restoration of soil moisture after alfalfa cultivation in the study regions, the rotation system potato (Solanum tuberosum) --> potato --> spring wheat (Triticum aestivum) could be adopted, and alfalfa could be cultivated again after 32-33 years.

  4. Virulence of Fusarium species to alfalfa seedlings

    Directory of Open Access Journals (Sweden)

    Krnjaja Vesna

    2005-01-01

    Full Text Available In in vitro conditions, virulence of 91 isolates of species Fusarium genus (F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. arthrosporioides, F. proliferatum, F. avenaceum, F. semitectum, F. tricinctum, F. sporotrichioides and F. graminearum towards alfalfa seedlings was investigated. Isolates of investigated species originated from diseased alfalfa plants collected on four locations in Serbia based on symptoms of wilting caused by fusarium and root rotting. Pathogenicity and virulence of investigated isolates of Fusarium spp. were determined by visual evaluation of inoculated seedlings of cultivars K28 in laboratory conditions. All isolated of investigated species had pathogenic effect on alfalfa seedlings, which expressed symptoms such as necrosis of root, moist rotting and "melting of seedlings". Colour of necrotic root tissue varied from light brown, brown, lipstick red to explicit black, depending on the Fusarium species. Strong virulence was established in 48 isolates, medium virulence in 31 and weak virulence in 12 isolates.

  5. The Undergraduate ALFALFA Team: Collaborative Research Projects

    Science.gov (United States)

    Cannon, John M.; Koopmann, Rebecca A.; Haynes, Martha P.; Undergraduate ALFALFA Team, ALFALFA Team

    2016-01-01

    The NSF-sponsored Undergraduate ALFALFA (Arecibo Legacy Fast ALFA) Team (UAT) has allowed faculty and students from a wide range of public and private colleges and especially those with small astronomy programs to learn how science is accomplished in a large collaboration while contributing to the scientific goals of a legacy radio astronomy survey. The UAT has achieved this through close collaboration with ALFALFA PIs to identify research areas accessible to undergraduates. In this talk we will summarize the main research efforts of the UAT, including multiwavelength followup observations of ALFALFA sources, the UAT Collaborative Groups Project, the Survey of HI in Extremely Low-mass Dwarfs (SHIELD), and the Arecibo Pisces-Perseus Supercluster Survey. This work has been supported by NSF grants AST-0724918/0902211, AST-075267/0903394, AST-0725380, and AST-1211005.

  6. Virulence of Fusarium species to alfalfa seedlings

    Directory of Open Access Journals (Sweden)

    Krnjaja Vesna

    2005-01-01

    Full Text Available In in vitro conditions, virulence of 91 isolates of species Fusarium genus (F. oxysporum, F. solani, F. acuminatum, F. equiseti, F. arthrosporioides, F. prolifera- tum, F. avenaceum, F. semitectum, F. tricinctum, F. sporotrichioides and F. graminearum towards alfalfa seedlings was investigated. Isolates of investigated species originated from diseased alfalfa plants collected at four locations in Serbia based on symptoms of wilting caused by Fusarium and root rotting. Pathogenicity and virulence of investigated isolates of Fusarium spp. were determined by visual evaluation of inoculated seedlings of cultivar K28 in laboratory conditions. All isolated of investigated species had pathogenic effect on alfalfa seedlings which expressed symptoms such as necrosis of root, moist rotting and "melting of seedlings". Colour of necrotic root tissue varied from light brown, brown lipstick red to explicit black, depending on the Fusarium species. Strong virulence was established in 48 isolates, medium virulence in 31 and weak virulence in 12 isolates.

  7. The qualitative identification of different alfalfa breed in same forage series by the terahertz spectroscopy

    Science.gov (United States)

    Wang, Fang; Guo, Shuai

    2016-01-01

    In order to enriching the means of discriminating alfalfa and achieving the goal of nondestructive testing, terahertz time-domain spectroscopy (THz-TDS) was applied to explore and reveal the property characteristic of different alfalfa varieties in the same series. Six kinds of alfalfa were prepared for experiment and analysis, and these samples are classed as two series, namely caoyuan series and gannong series. In the result, the time-domain spectra were tested, and then the refractive indices and absorption coefficients was calculated, respectively. These spectrums showed an apparent difference between these two series, and to verify this classification, two statistical methods, partial least squares (PLS) and cluster analysis (CA), were performed to investigate. Finally, these methods yielded a classification result, and we found it classified gannong series and caoyuan series. All these result showed THz technology combined with statistical method can be an effective method for nondestructive identification of alfalfa breed with tiny different properties, and lay a foundation for establishing a forage database.

  8. Molybdate in Rhizobial Seed-Coat Formulations Improves the Production and Nodulation of Alfalfa.

    Science.gov (United States)

    Zhou, Jiqiong; Deng, Bo; Zhang, Yingjun; Cobb, Adam B; Zhang, Zhao

    2017-01-01

    Rhizobia-legume symbiosis is the most well researched biological nitrogen fixation system. Coating legume seeds with rhizobia is now a recognized practical measure for improving the production of legume corp. However, the efficacy of some commercial rhizobia inoculants cannot be guaranteed in China due to the low rate of live rhizobia in these products. A greenhouse experiment was conducted to assess the effects of different rhizobial inoculant formulations on alfalfa productivity and nitrogen fixation. Two rhizobia strains, (ACCC17631 and ACCC17676), that are effective partners with alfalfa variety Zhongmu No. 1 were assessed with different concentrations of ammonium molybdate in seed-coat formulations with two different coating adhesives. Our study showed that the growth, nodulation, and nitrogen fixation ability of the plants inoculated with the ACCC17631 rhizobial strain were greatest when the ammonium molybdate application was0.2% of the formulation. An ammonium molybdate concentration of 0.1% was most beneficial to the growth of the plants inoculated with the ACCC17676 rhizobial strain. The sodium carboxymethyl cellulose and sodium alginate, used as coating adhesives, did not have a significant effect on alfalfa biomass and nitrogen fixation. However, the addition of skimmed milk to the adhesive improved nitrogenase activity. These results demonstrate that a new rhizobial seed-coat formulation benefitted alfalfa nodulation and yield.

  9. Molybdate in Rhizobial Seed-Coat Formulations Improves the Production and Nodulation of Alfalfa

    Science.gov (United States)

    Zhou, Jiqiong; Deng, Bo; Zhang, Yingjun; Cobb, Adam B.; Zhang, Zhao

    2017-01-01

    Rhizobia-legume symbiosis is the most well researched biological nitrogen fixation system. Coating legume seeds with rhizobia is now a recognized practical measure for improving the production of legume corp. However, the efficacy of some commercial rhizobia inoculants cannot be guaranteed in China due to the low rate of live rhizobia in these products. A greenhouse experiment was conducted to assess the effects of different rhizobial inoculant formulations on alfalfa productivity and nitrogen fixation. Two rhizobia strains, (ACCC17631 and ACCC17676), that are effective partners with alfalfa variety Zhongmu No. 1 were assessed with different concentrations of ammonium molybdate in seed-coat formulations with two different coating adhesives. Our study showed that the growth, nodulation, and nitrogen fixation ability of the plants inoculated with the ACCC17631 rhizobial strain were greatest when the ammonium molybdate application was0.2% of the formulation. An ammonium molybdate concentration of 0.1% was most beneficial to the growth of the plants inoculated with the ACCC17676 rhizobial strain. The sodium carboxymethyl cellulose and sodium alginate, used as coating adhesives, did not have a significant effect on alfalfa biomass and nitrogen fixation. However, the addition of skimmed milk to the adhesive improved nitrogenase activity. These results demonstrate that a new rhizobial seed-coat formulation benefitted alfalfa nodulation and yield. PMID:28099471

  10. De novo characterization of fall dormant and nondormant alfalfa (Medicago sativa L.) leaf transcriptome and identification of candidate genes related to fall dormancy.

    Science.gov (United States)

    Zhang, Senhao; Shi, Yinghua; Cheng, Ningning; Du, Hongqi; Fan, Wenna; Wang, Chengzhang

    2015-01-01

    Alfalfa (Medicago sativa L.) is one of the most widely cultivated perennial forage legumes worldwide. Fall dormancy is an adaptive character related to the biomass production and winter survival in alfalfa. The physiological, biochemical and molecular mechanisms causing fall dormancy and the related genes have not been well studied. In this study, we sequenced two standard varieties of alfalfa (dormant and non-dormant) at two time points and generated approximately 160 million high quality paired-end sequence reads using sequencing by synthesis (SBS) technology. The de novo transcriptome assembly generated a set of 192,875 transcripts with an average length of 856 bp representing about 165.1 Mb of the alfalfa leaf transcriptome. After assembly, 111,062 (57.6%) transcripts were annotated against the NCBI non-redundant database. A total of 30,165 (15.6%) transcripts were mapped to 323 Kyoto Encyclopedia of Genes and Genomes pathways. We also identified 41,973 simple sequence repeats, which can be used to generate markers for alfalfa, and 1,541 transcription factors were identified across 1,350 transcripts. Gene expression between dormant and non-dormant alfalfa at different time points were performed, and we identified several differentially expressed genes potentially related to fall dormancy. The Gene Ontology and pathways information were also identified. We sequenced and assembled the leaf transcriptome of alfalfa related to fall dormancy, and also identified some genes of interest involved in the fall dormancy mechanism. Thus, our research focused on studying fall dormancy in alfalfa through transcriptome sequencing. The sequencing and gene expression data generated in this study may be used further to elucidate the complete mechanisms governing fall dormancy in alfalfa.

  11. Assessment of stress tolerance, productivity, and forage quality in T1 transgenic alfalfa co-overexpressing ZxNHX and ZxVP1-1 from Zygophyllum xanthoxylum

    OpenAIRE

    Peng Kang; Ai-Ke Bao; Tanweer Kumar; Ya-Qing Pan; Zhulatai Bao; Fei Wang; Suo-Min Wang

    2016-01-01

    Salinization, desertification, and soil nutrient deprivation are threatening the production of alfalfa (Medicago sativa L.) in northern China. We have previously generated T0 transgenic alfalfa co-overexpressing Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 genes with enhanced salt and drought tolerance. To further develop this excellent breeding material into the new forage cultivar, stress tolerance, productivity, and forage quality of T1 transgenic alfalfa (GM) were assessed in this study. The...

  12. Towards a generic variable column length method development strategy for samples with a large variety in polarity.

    Science.gov (United States)

    Cabooter, Deirdre; Choikhet, Konstantin; Lestremau, François; Dittmann, Monika; Desmet, Gert

    2014-11-12

    The development of a novel set-up for the sequential analysis of compounds with a large variety in polarity on HILIC and reversed-phase columns, coupled in series, is discussed. For this purpose, a commercially available ultra-high performance LC system, equipped with two switching valves is employed. The switching valves allow connecting the HILIC and reversed-phase columns either in series or in parallel to the system. An interface to couple the HILIC and reversed-phase columns is developed and optimized. The sample is first injected onto a HILIC column. Apolar compounds in the sample are not retained and will elute close to or within the void volume of the HILIC column. Accurate switching of the valves allows redirecting these compounds towards a trap loop while more polar compounds are retained and separated on the HILIC column. After separation and detection of the polar compounds, the configuration of the valves is switched again to direct the apolar compounds from the trap loop towards a reversed-phase column for separation. To deal with the incompatibility of the mobile phases of HILIC and reversed-phase column separations, commercially available Jet weaver mixers are included in the set-up to allow for an intermediate solvent exchange. The proof-of-concept is demonstrated for the analysis of pharmaceuticals that can be found in waste water and surface water. It is demonstrated that the set-up provides robust analyses with peak capacities that are intermediate to one-dimensional and two-dimensional separations.

  13. Short communication. Nitrogen content of residual alfalfa taproots under irrigation

    Directory of Open Access Journals (Sweden)

    S. Cela

    2013-04-01

    Full Text Available The decomposition of alfalfa (Medicago sativa L. residues can provide significant amounts of N to subsequent crops, but most of the data on this subject has been obtained from 1-2 year old alfalfa stands. The objective of this study was to determine the biomass of alfalfa taproots and their N content in irrigated alfalfa stands that are more than 2 years old. Twenty-two commercial irrigated alfalfa fields were evaluated in the Ebro Valley (Northeast Spain from 2006 to 2010. The taproot biomass in the arable layer (0 to 30 cm depth ranged from 1.8 to 10.1 Mg ha-1 and averaged 4.8 Mg ha-1. In contrast, the N concentration in alfalfa taproots was constant among fields and averaged 24.6 g N kg-1. The total amount of N contained in alfalfa taproots (0-30 cm depth ranged from 47 to 96 kg N ha-1 in 55% of the fields, ranged from 97 to 200 kg N ha-1 in 22% of the fields, and exceeded 200 kg N ha-1 in 23% of the fields. The N content of the irrigated alfalfa taproots studied here is in the upper range previously reported in other areas, mainly with younger alfalfa stands. Based on the current finding, a classification of the quality of irrigated alfalfa stands is proposed to improve the estimates of the residual-N effects of alfalfa on subsequent crops.

  14. USDA-ARS Plant Science Research Unit, St. Paul Alfalfa/Forage Research Program

    Science.gov (United States)

    The Plant Science Research Unit (PSRU) located at the University of Minnesota in St. Paul receives approximately $1.5 million to fund the research of six scientists who direct their research efforts toward developing new uses and improved traits for alfalfa. Our overarching goal is to develop alfalf...

  15. Flue gas desulfurization products as sulfur sources for alfalfa and soybean

    Energy Technology Data Exchange (ETDEWEB)

    Chen, L.M.; Dick, W.A.; Nelson, S. [Ohio State University, Wooster, OH (US). School of Natural Resources

    2005-02-01

    Sulfur deficiencies in soil are expected to increase due to growth of high-yielding crop varieties, use of S-free fertilizers, and removal of S from industrial emissions. Flue gas desulfurization (FGD) products, created when coal is burned and SO{sub 2} is removed from the flue gases, may serve as efficient S sources. However, there are few reports on their use for the enhancement of crop growth. Agricultural gypsum and two types of FGD products, that contain either vermiculite or perlite, were applied at 0, 16, and 67 kg S ha{sup -1} to an agricultural soil (Wooster silt loam, Typic Fragiudalf). Dry weight of a new planting of alfalfa (Medicago sativa L.) was increased up to 40% by the treatments of FGD products or gypsum compared with the untreated control. Gypsum and FGD products were also applied at 0, 8,16, and 24 kg S ha{sup -1} to five established alfalfa stands in different Ohio regions. Mean alfalfa yield was significantly (P {le} 0.05) increased by approximately 5.0% in 2001 and 6.0% in 2002 with the S treatments of FGD products or gypsum compared with the untreated control. Alfalfa yields for FGD products and gypsum treatments were similar. A slight positive yield response was observed for soybean (Glycine max L.) when soils were treated with S-containing materials. Soil and plant analyses were made to assess potential adverse environmental impacts and none were observed. Thus, these FGD products can be safely applied to agricultural soils as S sources and can improve alfalfa yields in S-deficient soils.

  16. Effects of Cutting Time, Ensiling Duration and Microbial Additives on Chemical Composition of Alfalfa Silage

    Directory of Open Access Journals (Sweden)

    M.H. Delavar

    2012-09-01

    Full Text Available This experiment was conducted to assess the effects of daytime cutting management (p.m. vs. a.m. cut, ensiling duration and adding microbial inoculants on chemical composition of alfalfa silage. For this, Second growth Alfalfa harvested at the early bud stage of development after a sunny day at sundown (about 1900 h; PM alfalfa, whereas the second half was cut next day at sunup (about 0800 h; AM alfalfa. After cutting, alfalfa Forage was chopped by using a chopper to a length of 8 to10 cm, and then ensiled without or with microbial additive as factorial experiment (2×2 with repeated measurement design. Silages were provided in laboratory silos (6 repeats in every treatment lined with two layers of plastic, after air exclusion. Silos were opened at 3, 10 and 30 day for determination of pH and other chemical analysis. The numerically lower pH of PM vs. AM silages indicates that the former forage was more extensively fermented possibly because of its increased total non structural carbohydrate (TNC concentration. Shifting alfalfa harvesting from sun up to sundown significantly decreased NDF% and ADF%, because of the dilution effect associated with increased concentrations of TNC in the former forage. The NPN content and N-NH3 concentration of the silages treated in the afternoon was lower compared with AM group. CP content decreased, but NDF, ADF, NPN and N-NH3 concentration increased during ensiling time. Silage pH decreased by using of microbial additive and ensiling time. Dry matter and nitrogen losses were lower in silages treated by microbial inoculants, and, increased with increasing fermentation time. It can be concluded that microbial additives and time of cutting can be used as proper way to improve fermentation situation and silage quality.

  17. ECONOMIC ANALYSIS OF ALFALFA INTEGRATED MANAGEMENT PRACTICES

    OpenAIRE

    Ward, Clement E.; Dowdy, Alan K.; Berberet, Richard C.; Stritzke, Jimmie F.

    1990-01-01

    Integrated pest management (IMP) initially focused on insect pest control. More recently, IPM encompasses a broader concept of management, one which crosses several disciplinary boundaries. This article reports results of research dealing with four integrated management decisions for alfalfa (cultivar selection, inset control, weed control, and end-of-season harvest options.

  18. Rapid phenotyping of alfalfa root system architecture

    Science.gov (United States)

    Root system architecture (RSA) influences the capacity of an alfalfa plant for symbiotic nitrogen fixation, nutrient uptake and water use efficiency, resistance to frost heaving, winterhardiness, and some pest and pathogen resistance. However, we currently lack a basic understanding of root system d...

  19. Potato leafhopper (Hemiptera: Cicadellidae) ecology and integrated pest management focused on alfalfa

    Science.gov (United States)

    Knowledge to date on biology of the potato leafhopper, Empoasca fabae (Harris), is summarized, including geographic distribution, development, migration, agricultural host plants, and the mechanism of injury to host plants. Damage to alfalfa, potato, soybean and snap bean, as well as treatment guide...

  20. Fungi isolated from the initial industrial soil planted with a mixture of alfalfa and grasses

    Directory of Open Access Journals (Sweden)

    Maria Kowalik

    2014-08-01

    Full Text Available Research were conducted during the years 1988-1990 on the "Machów" industrial waste dump. It was determined, that a mixture of alfalfa with grasses. introduced as first culture on the raw substrate of industrial wastes. was helpful for the development of soil fungi communities.

  1. Comparative studies on egg, meat, and semen qualities of native and improved chicken varieties developed for backyard poultry production.

    Science.gov (United States)

    Haunshi, Santosh; Doley, Sunil; Kadirvel, G

    2010-06-01

    The objective of the present study was to evaluate/compare the sensory attributes of eggs and meat, egg qualities, proximate composition of eggs, and semen qualities of slow growing native (Miri and Mizo-local) and fast growing improved chicken varieties (Gramapriya and Vanaraja) under hill ecosystem of northeastern India. Significantly higher egg weight, egg volume, and albumen volume were observed in Gramapriya followed by Vanaraja, Mizo-local, and Miri chickens. However, yolk volume was significantly higher in Vanaraja and Gramapriya varieties as compared to native chickens. Yolk to albumen ratio was significantly lower in Gramapriya as compared to Vanaraja and Miri chicken. Consumer liking of eggs for aroma, flavor, and overall acceptability of Miri, Mizo-local, and Vanaraja were significantly higher than that of Gramapriya. Genetic groups did not differ significantly in appearance and proximate composition of eggs. No significant differences were observed between various genetic groups for sensory attributes of meat samples. Semen volume was significantly (p < or = 0.01) lower while sperm concentration was significantly (p < or = 0.01) higher in native chicken as compared to the improved chicken varieties. However, pH, mass activity, sperm motility, and livability did not differ significantly among genetic groups although Mizo-local had significantly higher abnormal sperm count. The study concluded that the genetic groups with different growth rate differed significantly for various egg quality parameters and semen characteristics but not for sensory attributes of meat and proximate composition of eggs.

  2. Efficacy of a BVDV subunit vaccine produced in alfalfa transgenic plants.

    Science.gov (United States)

    Peréz Aguirreburualde, María Sol; Gómez, María Cristina; Ostachuk, Agustín; Wolman, Federico; Albanesi, Guillermo; Pecora, Andrea; Odeon, Anselmo; Ardila, Fernando; Escribano, José M; Dus Santos, María José; Wigdorovitz, Andrés

    2013-02-15

    Bovine viral diarrhea virus (BVDV) is considered an important cause of economic loss within bovine herds worldwide. In Argentina, only the use of inactivated vaccines is allowed, however, the efficacy of inactivated BVDV vaccines is variable due to its low immunogenicity. The use of recombinant subunit vaccines has been proposed as an alternative to overcome this difficulty. Different studies on protection against BVDV infection have focused the E2 protein, supporting its putative use in subunit vaccines. Utilization of transgenic plants expressing recombinant antigens for the formulation of experimental vaccines represents an innovative and cost effective alternative to the classical fermentation systems. The aim of this work was to develop transgenic alfalfa plants (Medicago sativa, L.) expressing a truncated version of the structural protein E2 from BVDV fused to a molecule named APCH, that target to antigen presenting cells (APCH-tE2). The concentration of recombinant APCH-tE2 in alfalfa leaves was 1 μg/g at fresh weight and its expression remained stable after vegetative propagation. A methodology based an aqueous two phases system was standardized for concentration and partial purification of APCH-tE2 from alfalfa. Guinea pigs parentally immunized with leaf extracts developed high titers of neutralizing antibodies. In bovine, the APCH-tE2 subunit vaccine was able to induce BVDV-specific neutralizing antibodies. After challenge, bovines inoculated with 3 μg of APCH-tE2 produced in alfalfa transgenic plants showed complete virological protection.

  3. Alfalfa (Medicago sativa L./maize (Zea mays L. intercropping provides a feasible way to improve yield and economic incomes in farming and pastoral areas of northeast China.

    Directory of Open Access Journals (Sweden)

    Baoru Sun

    Full Text Available Given the growing challenges to food and eco-environmental security as well as sustainable development of animal husbandry in the farming and pastoral areas of northeast China, it is crucial to identify advantageous intercropping modes and some constraints limiting its popularization. In order to assess the performance of various intercropping modes of maize and alfalfa, a field experiment was conducted in a completely randomized block design with five treatments: maize monoculture in even rows, maize monoculture in alternating wide and narrow rows, alfalfa monoculture, maize intercropped with one row of alfalfa in wide rows and maize intercropped with two rows of alfalfa in wide rows. Results demonstrate that maize monoculture in alternating wide and narrow rows performed best for light transmission, grain yield and output value, compared to in even rows. When intercropped, maize intercropped with one row of alfalfa in wide rows was identified as the optimal strategy and the largely complementary ecological niches of alfalfa and maize were shown to account for the intercropping advantages, optimizing resource utilization and improving yield and economic incomes. These findings suggest that alfalfa/maize intercropping has obvious advantages over monoculture and is applicable to the farming and pastoral areas of northeast China.

  4. Alfalfa (Medicago sativa L.)/maize (Zea mays L.) intercropping provides a feasible way to improve yield and economic incomes in farming and pastoral areas of northeast China.

    Science.gov (United States)

    Sun, Baoru; Peng, Yi; Yang, Hongyu; Li, Zhijian; Gao, Yingzhi; Wang, Chao; Yan, Yuli; Liu, Yanmei

    2014-01-01

    Given the growing challenges to food and eco-environmental security as well as sustainable development of animal husbandry in the farming and pastoral areas of northeast China, it is crucial to identify advantageous intercropping modes and some constraints limiting its popularization. In order to assess the performance of various intercropping modes of maize and alfalfa, a field experiment was conducted in a completely randomized block design with five treatments: maize monoculture in even rows, maize monoculture in alternating wide and narrow rows, alfalfa monoculture, maize intercropped with one row of alfalfa in wide rows and maize intercropped with two rows of alfalfa in wide rows. Results demonstrate that maize monoculture in alternating wide and narrow rows performed best for light transmission, grain yield and output value, compared to in even rows. When intercropped, maize intercropped with one row of alfalfa in wide rows was identified as the optimal strategy and the largely complementary ecological niches of alfalfa and maize were shown to account for the intercropping advantages, optimizing resource utilization and improving yield and economic incomes. These findings suggest that alfalfa/maize intercropping has obvious advantages over monoculture and is applicable to the farming and pastoral areas of northeast China.

  5. The Undergraduate ALFALFA Team: Outcomes for Over 250 Undergraduate Participants

    Science.gov (United States)

    Troischt, Parker; Koopmann, Rebecca A.; Haynes, Martha P.; ALFALFA Team

    2016-01-01

    The NSF-sponsored Undergraduate ALFALFA (Arecibo Legacy Fast ALFA) Team (UAT) is a consortium of 19 institutions founded to promote undergraduate research and faculty development within the extragalactic ALFALFA HI blind survey project and follow-up programs. In this talk we present outcomes for the more than 250 undergraduate students who have who have participated in the program during the 8 years of funding. 40% of these students have been women and members of underrepresented groups. To date 148 undergraduate students have attended annual workshops at Arecibo Observatory, interacting with faculty, graduate students, their peers, and Arecibo staff in lectures, group activities, tours, and observing runs. Team faculty have supervised 159 summer research projects and 120 academic year (e.g., senior thesis) projects. 68 students have traveled to Arecibo Observatory for observing runs and 55 have presented their results at national meetings such as the AAS. Through participation in the UAT, students are made aware of career paths they may not have previously considered. More than 90% of alumni are attending graduate school and/or pursuing a career in STEM. 42% of those pursuing graduate degrees in Physics or Astronomy are women. This work has been supported by NSF grants AST-0724918/0902211, AST-075267/0903394, AST-0725380, and AST-1211005

  6. [Development and study of spring bread wheat variety Pamyati Maystrenko with introgression of genetic material from synthetic hexaploid Triticum timopheevii zhuk. x Aegilops tauschii Coss].

    Science.gov (United States)

    Laikova, L I; Belan, I A; Badaeva, E D; Posseeva, L P; Shepelev, S S; Shumny, V K; Pershina, L A

    2013-01-01

    Synthetic hexaploids are bridges for transferring new genes that determine resistance to stress factors from wild-type species to bread wheat. In the present work, the method of developing the spring bread wheat variety Pamyati Maystrenko and the results of its study are described. This variety was obtained using one of the immune lines produced earlier via the hybridization of the spring bread wheat variety Saratovskaya 29 with the synthetic hexaploid T. timopheevii Zhuk. x Ae. tauschii Coss. The C-staining of chromosomes in the Pamyati Maystrenko variety revealed substitutions of 2B and 6B chromosomes by the homeologous chromosomes of the G genome of T. timopheevii and the substitution of chromosome 1D by an orthologous chromosome ofAe. tauschii. It was found that this variety is characterized by resistance to leaf and stem rust, powdery mildew, and loose smut as well as by high grain and bread-making qualities. The role of the alien genetic material introgressed into the bread-wheat genome in the expression of adaptive and economically valuable traits in the Pamyati Maystrenko variety is discussed.

  7. Assessing of the contributions of pod photosynthesis to carbon acquisition of seed in alfalfa (Medicago sativa L.)

    Science.gov (United States)

    Zhang, Wenxu; Mao, Peisheng; Li, Yuan; Wang, Mingya; Xia, Fangshan; Wang, Hui

    2017-01-01

    The distribution of carbon from a branch setting pod in alfalfa was investigated during the seed development of seeds to determine the relative contribution of pod and leaf photoassimilates to the total C balance and to investigate the partitioning of these photoassimilates to other plant organs. A 13Clabeling procedure was used to label C photoassimilates of pods and leaves in alfalfa, and the Δ13C values of a pod, leaves, a section of stem and roots were measured during seed development on day 10, 15, 20 and 25 after labeling of the pod. The results showed that the alfalfa pod had photosynthetic capacity early in the development of seeds, and that pod photosynthesis could provide carbon to alfalfa organs including seeds, pods, leaves, stems and roots, in addition to leaf photosynthesis. Photosynthesis in the pod affected the total C balance of the alfalfa branch with the redistribution of a portion of pod assimilates to other plant organs. The assimilated 13C of the pod was used for the growth requirements of plant seeds and pods. The requirements for assimilated C came primarily from the young pod in early seed development, with later requirements provided primarily from the leaf. PMID:28169330

  8. Assessing of the contributions of pod photosynthesis to carbon acquisition of seed in alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Zhang, Wenxu; Mao, Peisheng; Li, Yuan; Wang, Mingya; Xia, Fangshan; Wang, Hui

    2017-02-07

    The distribution of carbon from a branch setting pod in alfalfa was investigated during the seed development of seeds to determine the relative contribution of pod and leaf photoassimilates to the total C balance and to investigate the partitioning of these photoassimilates to other plant organs. A (13)Clabeling procedure was used to label C photoassimilates of pods and leaves in alfalfa, and the Δ(13)C values of a pod, leaves, a section of stem and roots were measured during seed development on day 10, 15, 20 and 25 after labeling of the pod. The results showed that the alfalfa pod had photosynthetic capacity early in the development of seeds, and that pod photosynthesis could provide carbon to alfalfa organs including seeds, pods, leaves, stems and roots, in addition to leaf photosynthesis. Photosynthesis in the pod affected the total C balance of the alfalfa branch with the redistribution of a portion of pod assimilates to other plant organs. The assimilated (13)C of the pod was used for the growth requirements of plant seeds and pods. The requirements for assimilated C came primarily from the young pod in early seed development, with later requirements provided primarily from the leaf.

  9. An optimization model to design and manage subsurface drip irrigation system for alfalfa

    Science.gov (United States)

    Kandelous, M.; Kamai, T.; Vrugt, J. A.; Simunek, J.; Hanson, B.; Hopmans, J. W.

    2010-12-01

    Subsurface drip irrigation (SDI) is one of the most efficient and cost-effective methods for watering alfalfa plants. Lateral installation depth and distance, emitter discharge, and irrigation time and frequency of SDI, in addition to soil and climatic conditions affect alfalfa’s root water uptake and yield. Here we use a multi-objective optimization approach to find optimal SDI strategies. Our approach uses the AMALGAM evolutionary search method, in combination with the HYDRUS-2D unsaturated flow model to maximize water uptake by alfalfa’s plant roots, and minimize loss of irrigation and drainage water to the atmosphere or groundwater. We use a variety of different objective functions to analyze SDI. These criteria include the lateral installation depth and distance, the lateral discharge, irrigation duration, and irrigation frequency. Our framework includes explicit recognition of the soil moisture status during the simulation period to make sure that the top soil is dry for harvesting during the growing season. Initial results show a wide spectrum of optimized SDI strategies for different root distributions, soil textures and climate conditions. The developed tool should be useful in helping farmers optimize their irrigation strategy and design.

  10. Overexpressing Arabidopsis ABF3 increases tolerance to multiple abiotic stresses and reduces leaf size in alfalfa.

    Science.gov (United States)

    Wang, Zhi; Su, Guoxia; Li, Min; Ke, Qingbo; Kim, Soo Young; Li, Hongbing; Huang, Jin; Xu, Bingcheng; Deng, Xi-Ping; Kwak, Sang-Soo

    2016-12-01

    Arabidopsis ABSCISIC ACID-RESPONSIVE ELEMENT-BINDING FACTOR 3 (ABF3), a bZIP transcription factor, plays an important role in regulating multiple stress responses in plants. Overexpressing AtABF3 increases tolerance to various stresses in several plant species. Alfalfa (Medicago sativa L.), one of the most important perennial forage crops worldwide, has high yields, high nutritional value, and good palatability and is widely distributed in irrigated and semi-arid regions throughout the world. However, drought and salt stress pose major constraints to alfalfa production. In this study, we developed transgenic alfalfa plants (cv. Xinjiang Daye) expressing AtABF3 under the control of the sweetpotato oxidative stress-inducible SWPA2 promoter (referred to as SAF plants) via Agrobacterium tumefaciens-mediated transformation. After drought stress treatment, we selected two transgenic lines with high expression of AtABF3, SAF5 and SAF6, for further characterization. Under normal conditions, SAF plants showed smaller leaf size compared to non-transgenic (NT) plants, while no other morphological changes were observed. Moreover, SAF plants exhibited enhanced drought stress tolerance and better growth under drought stress treatment, which was accompanied by a reduced transpiration rate and lower reactive oxygen species contents. In addition, SAF plants showed an increased tolerance to salt and oxidative stress. Therefore, these transgenic AtABF3 alfalfa plants might be useful for breeding forage crops with enhanced tolerance to environmental stress for use in sustainable agriculture on marginal lands.

  11. The Relationship Among Photoinhibition, Photooxidation and Early Aging at a Late Development Stage in Different Varieties with High Yield

    Institute of Scientific and Technical Information of China (English)

    JIAO De-mao; LI Xia; HUANG Xue-qing; JI Ben-hua

    2002-01-01

    Using six high yield varieties from different ecological districts in China the parameters of Chl fluorescence, the performance of membrane lipid peroxidation and the reduction of Chl content in flag leaf under natural condition at the later developmental stages (from heading stage to mature stage). The results showed that Fv/Fm, decreased gradually, the excessive light energy led to the accumulation of active oxygen O2-, H2O2 and the product of membrane-lipid peroxidation, MDA, which resulted in the reduction of Chl content and early aging due to photooxidation during the course of senescence of flag leaf. This phenomenon varied obviously in rice varieties. When comparina japonica tolerant to photooxidation, Fv/Fm in indica shanyou 63 susceptible to photoxidation decreased significantly. An increase of active oxygen and a sharp drop of Chl content, resulted in "yellowish" early aging and influenced filling and setting of rice grain. The mechanism on early aging in indica was related to light and temperature conditions in filling stages. On a fine day (above 25℃ ), PS Ⅱ reaction center exhibited a dynamic change on revisable inactivation. Under strong midday light, PS Ⅱ function in indica exhibited obvious down-regulation and photoinhibition; Under strong light with low temperature, PS Ⅱ resulted in photodamage, showing early aging, which were related to the degradation of PS Ⅱ - D1 Protein and the inhibition of the endogenous protecting system such as Xanthophyll cycle and the enzymes of scavenging active oxygen. The results suggested that for a view of high-yield breeding, on the basis of a good type-plant, giving consideration to the utilization of heterosis and resistance to early aging, selecting japonica or a sterile line with japonice genotype as maternal was a breeding strategy worthy of being paid more attention.

  12. Piecewise algebraic varieties

    Institute of Scientific and Technical Information of China (English)

    WANG Renhong; ZHU Chungang

    2004-01-01

    The piecewise algebraic variety is a generalization of the classical algebraic variety. This paper discusses some properties of piecewise algebraic varieties and their coordinate rings based on the knowledge of algebraic geometry.

  13. Quiver Varieties and Branching

    Directory of Open Access Journals (Sweden)

    Hiraku Nakajima

    2009-01-01

    Full Text Available Braverman and Finkelberg recently proposed the geometric Satake correspondence for the affine Kac-Moody group Gaff [Braverman A., Finkelberg M., arXiv:0711.2083]. They conjecture that intersection cohomology sheaves on the Uhlenbeck compactification of the framed moduli space of Gcpt-instantons on $R^4/Z_r$ correspond to weight spaces of representations of the Langlands dual group $G_{aff}^{vee}$ at level $r$. When $G = SL(l$, the Uhlenbeck compactification is the quiver variety of type $sl(r_{aff}$, and their conjecture follows from the author's earlier result and I. Frenkel's level-rank duality. They further introduce a convolution diagram which conjecturally gives the tensor product multiplicity [Braverman A., Finkelberg M., Private communication, 2008]. In this paper, we develop the theory for the branching in quiver varieties and check this conjecture for $G = SL(l$.

  14. Combined effects of ozone and water stress on alfalfa growth and yield. [Medicago sativa L

    Energy Technology Data Exchange (ETDEWEB)

    Temple, P.J.; Benoit, L.F.; Lennox, R.W.; Reagan, C.A.; Taylor, O.C.

    A 2-yr study (1984 and 1985) designed to determine the interactive effects of ozone (O/sub 3/) and water stress on alfalfa (Medicago sativa L. cv. WL-514) was conducted in Shafter, CA. The objectives of this study were to develop O/sub 3/ dose-yield response functions for alfalfa, to determine how water stress could alter these functions, and to describe the cumulative effects of multiple-year exposures to O/sub 3/ on this perennial crop. Field-grown alfalfa, raised under normally irrigated (NI) or water-stressed (WS) conditions in open-top chambers, was exposed to five O/sub 3/ treatments including charcoal-filtered (CF), nonfiltered (NF), and NF plus an additional 33, 67, or 100% of ambient O/sub 3/ concentrations. Ambient O/sub 3/ concentrations averaged 0.049 ..mu..L L/sup -1/ in 1984 and 0.042 ..mu..L L/sup -1/ in 1985 for the seasonal 12-h (0900-2100) means from April to October. Water stress reduced total seasonal yield about 10% in 1984 and 27% in 1985. Ozone significantly reduced yields in both years, and the interactions between O/sub 3/ and water stress was statistically significant in 1985 and for combined 1984 and 1985 years. Ozone dose-alfalfa yield loss functions were homogeneous between 1984 and 1985 and no evidence of a cumulative effect of multiple-year exposure to O/sub 3/ was found on top growth. However, crown (underground stem) weights were significantly reduced by O/sub 3/, suggesting that continued exposure to O/sub 3/ could shorten the productive life of alfalfa stands, in addition to its effect on yield.

  15. Persistence and diversity of rhizobial bacteria nodulating alfalfa

    Science.gov (United States)

    Most alfalfa seed is treated with an inoculant consisting of several strains of the nitrogen fixing bacterium Sinorhizobium meliloti to enhance nodulation of seedlings. One strategy for increasing alfalfa forage yields, particularly in less fertile sites, is selection and use of highly competitive a...

  16. Broadening the U.S. alfalfa germplasm base

    Science.gov (United States)

    Over 4000 alfalfa (Medicago sativa L.) plant introductions (PIs) exist in the USDA-ARS National Plant Germplasm System (NPGS). NAAIC has discussed/proposed pre-breeding efforts to utilize this germplasm for creating pre-commercial alfalfa germplasm. Funding constraints have been one impediment to th...

  17. Complete genome sequence of the alfalfa latent virus

    Science.gov (United States)

    Alfalfa latent virus (ALV) is a member of the carlavirus group and occurs symptomlessly in alfalfa (Medicago sativa). In the US it is prevalent in Nebraska and Wisconsin. The virus is recognized as a strain of Pea streak virus (PeSV) So far, no complete genomic sequence of PSV or ALV is availab...

  18. Effects of the susceptibility of rice varieties to Sogatella furcifera on nymphal development and reproduction of Microvelia horvathi through a food chain

    Institute of Scientific and Technical Information of China (English)

    TIE-GUANG XIAO; JIANG-XIA TANG

    2007-01-01

    The effects of four rice varieties highly susceptible ('Xiang-Zao-Xian31' and 'Jia-Yu 164' ), moderately susceptible ('Wei-You402'), and resistant ('Zhu-Liang-You02') to the whitebacked planthopper Sogatella furcifera on the nymphal development, reproduction and survival of Microvelia horoathi were investigated through a food chain using Sogatella furcifera as herbivore prey. Significant longer nymphal developmental time were observed in the 1st, 4th, 5th instar and whole nymphal stage of M. horvathi raised on S.furcifera fed 'ZLY02' and 'WY402' than those of the other two varieties. M. horvathi raised on S. furcifera fed resistant 'ZLY02' showed a significant lower survival of whole nymphal stage than those fed other three susceptible varieties. Moreover, M. horvathi raised on S. furcifera fed resistant 'ZLY02' had shorter female longevity and lower lifetime fecundity than those of on susceptible 'XZX31'. Our results suggest that the S. furcifera-resistant rice variety 'ZLY02' had a negative effect on the growth and development of the predator M. horvathi through a food chain.

  19. Effects of diets containing alfalfa hay or barley flour mixed alfalfa silage on feeding behavior, productivity, rumen fermentation and blood metabolites in lactating cows.

    Science.gov (United States)

    Khadem, Ali-Akbar; Sharifi, Majid; Afzalzadeh, Ahmad; Rezaeian, Mohammad

    2009-08-01

    The effects of barley flour on the fermentation parameters of alfalfa silage and on the productivity of dairy cows were investigated. Alfalfa forage was ensiled either with or without barley flour. Barley flour was soaked in water for 24 h before being mixed with alfalfa (12 kg: 100 kg dry matter bases) at ensiling. Eighteen multi-parous cows were assigned to three equal treatment groups using a completely randomized design. Three isocaloric and isonitrogenous total mixed rations containing alfalfa hay, ordinary alfalfa silage or barley flour mixed alfalfa silage were then prepared. The concentrations of ammonia nitrogen, acetic acid and butyric acid were lower in barley flour mixed alfalfa silage compared to that in ordinary alfalfa silage but the concentration of lactic acid was lower in the ordinary alfalfa silage. Feeding behavior, milk yield and composition, ruminal fermentation and blood metabolites were measured. Although dry matter intake and milk production were not affected, the effect of preparation of alfalfa influenced feeding behavior and rumen fermentation parameters. Cows on alfalfa silage diets spent longer ruminating compared to those fed alfalfa hay. The ruminal ammonia nitrogen and blood urea were affected by ensiling (alfalfa hay versus alfalfa silages) while both parameters were lower in cows fed on barley flour mixed alfalfa silage than those fed on ordinary silage. Although similar blood glucose was recorded for cows fed on alfalfa silages, it was higher in cows fed on alfalfa hay. It is concluded that the addition of barely flour when making alfalfa silage may improve both the fermentation process during ensilage and the ruminal ammonia nitrogen utilization with no significant effects on productivity.

  20. Cytotoxicity and apoptosis induced by alfalfa (Medicago sativa) leaf extracts in sensitive and multidrug-resistant tumor cells.

    Science.gov (United States)

    Gatouillat, Grégory; Magid, Abdulmagid Alabdul; Bertin, Eric; Okiemy-Akeli, Marie-Genevieve; Morjani, Hamid; Lavaud, Catherine; Madoulet, Claudie

    2014-01-01

    Alfalfa (Medicago sativa) has been used to cure a wide variety of ailments. However, only a few studies have reported its anticancer effects. In this study, extracts were obtained from alfalfa leaves and their cytotoxic effects were assessed on several sensitive and multidrug-resistant tumor cells lines. Using the mouse leukaemia P388 cell line and its doxorubicin-resistant counterpart (P388/DOX), we showed that the inhibition of cell growth induced by alfalfa leaf extracts was mediated through the induction of apoptosis, as evidenced by DNA fragmentation analysis. The execution of programmed cell death was achieved via the activation of caspase-3, leading to PARP cleavage. Fractionation of toluene extract (To-1), the most active extract obtained from crude extract, led to the identification of 3 terpene derivatives and 5 flavonoids. Among them, (-)-medicarpin, (-)-melilotocarpan E, millepurpan, tricin, and chrysoeriol showed cytotoxic effects in P388 as well as P388/DOX cells. These results demonstrate that alfalfa leaf extract may have interesting potential in cancer chemoprevention and therapy.

  1. Intended release and actual retention of alfalfa leafcutting bees (hymenoptera: megachilidae) for pollination in commercial alfalfa seed fields

    Science.gov (United States)

    Low, medium, and high stocking densities of Megachile rotundata, the alfalfa leafcutting bee, were released over four years in three research plots of Utah alfalfa planted at seed-production rates. A low number of bees (46-79% of released) survived the incubation and field emergence processes, and ...

  2. Fixed-precision sequential sampling plans for estimating alfalfa caterpillar, Colias lesbia, egg density in alfalfa, Medicago sativa, fields in Córdoba, Argentina.

    Science.gov (United States)

    Serra, Gerardo V; Porta, Norma C La; Avalos, Susana; Mazzuferi, Vilma

    2013-01-01

    The alfalfa caterpillar, Colias lesbia (Fabricius) (Lepidoptera: Pieridae), is a major pest of alfalfa, Medicago sativa L. (Fabales: Fabaceae), crops in Argentina. Its management is based mainly on chemical control of larvae whenever the larvae exceed the action threshold. To develop and validate fixed-precision sequential sampling plans, an intensive sampling programme for C. lesbia eggs was carried out in two alfalfa plots located in the Province of Córdoba, Argentina, from 1999 to 2002. Using Resampling for Validation of Sampling Plans software, 12 additional independent data sets were used to validate the sequential sampling plan with precision levels of 0.10 and 0.25 (SE/mean), respectively. For a range of mean densities of 0.10 to 8.35 eggs/sample, an average sample size of only 27 and 26 sample units was required to achieve a desired precision level of 0.25 for the sampling plans of Green and Kuno, respectively. As the precision level was increased to 0.10, average sample size increased to 161 and 157 sample units for the sampling plans of Green and Kuno, respectively. We recommend using Green's sequential sampling plan because it is less sensitive to changes in egg density. These sampling plans are a valuable tool for researchers to study population dynamics and to evaluate integrated pest management strategies.

  3. Genetics of growth habit and development of new coleus (Solenostemon scutellarioides (L.) Codd) varieties with trailing habit and bright color.

    Science.gov (United States)

    Nguyen, Phuong; Quesenberry, Kenneth; Clark, David

    2008-01-01

    A high level of genetic variability for growth habit types is observed in tetraploid, cultivated coleus (Solenostemon scutellarioides (L.) Codd). Very few cultivars with trailing habit exist, and those that are available generally have dark green or purple foliage color. Coleus with trailing growth habit is desirable as it increases its utility for use in hanging baskets, mixed containers, and as ground cover. There is a lack of published information on the genetic mechanism controlling growth habit and the development of new trailing types with orange colors. Two commercial cultivars, "Red Trailing Queen" (RQ) with trailing habit and "Sedona" (S) with upright stature, were selfed and crossed (RQ x S) to produce self and F(1) populations. F(2) populations were produced by selfing plants in the F(1) population. For each population analyzed, growth habit was rated on a visual 1-5 phenotypic scale, where 1 = upright, 2 = semi-upright, 3 = prostrate, 4 = semitrailing, and 5 = trailing. Genotypes were assigned to each phenotype, assuming that upright was dominant to trailing. In this study, growth habit was observed to be controlled by a single gene (U) with additive effects, with upright growth habit designated with a UUUU genotype and trailing growth habit designated with a uuuu genotype. In addition, foliage color was rated on a visual 1-5 phenotypic scale, and purple foliage color was found to be dominant to yellow-orange color. Several new coleus selections with trailing growth habit and orange foliage color were successfully developed.

  4. 不同苜蓿品种建植当年田间生长发育与根系特征的比较%A comparative study on growing development and root characteristics of different kinds of one-year-old alfalfa

    Institute of Scientific and Technical Information of China (English)

    纪荣花; 于磊; 鲁为华; 艾尼娃尔·艾合买提

    2011-01-01

    Productivity and root characteristics of eight one-year-old alfalfa varieties were studied.The results showed that the growth potential of Phabulous,Aergangjin,WL-323,Ningmuyihao and Three Profits surpass Qinglai,Landmark 2 and Alfaking;the sensitivity of environment was Landmark 2AlfakingQinglaiWL-323NingmuyihaoAergangjingThreeProfitsPhabulous.The matter accumulation rule on eight alfalfa varieties was "slow-fast-slow-fast".There were differences on root characteristics.Matter accumulation was highly correlating with root volum,crown diameter,taproot diameter underground 0~10 cm depth(P0.01),and with crown branch number,taproot diameter underground 10~20 cm,20~30 cm,lower than 30 cm depth,was positive correlation.%以8个苜蓿品种为研究对象,对其建植当年的生长速度、物质积累动态以及主根直径、根颈特性、根体积进行研究。结果表明,8个苜蓿品种在建植当年整个生育期的物质积累动态均表现为"慢-快-慢-快"的规律,但是不同品种的生长潜力及对环境敏感性存在差异,其中生长潜力较大且对环境敏感性小的品种有皇冠、阿尔冈金、WL-323、宁苜一号、三得利。参试苜蓿品种各根系参数间也存在差异,根系发育较好的有三得利、青睐、宁苜一号、WL-323。对物质积累与根系各指标的相关性分析表明,苜蓿地上部分生长发育与根系特性密切相关。

  5. Development and Testing of Cool-Season Grass Species, Varieties and Hybrids for Biomass Feedstock Production in Western North America

    Directory of Open Access Journals (Sweden)

    Steven R. Larson

    2017-01-01

    Full Text Available Breeding of native cool-season grasses has the potential to improve forage production and expand the range of bioenergy feedstocks throughout western North America. Basin wildrye (Leymus cinereus and creeping wildrye (Leymus triticoides rank among the tallest and most rhizomatous grasses of this region, respectively. The objectives of this study were to develop interspecific creeping wildrye (CWR × basin wildrye (BWR hybrids and evaluate their biomass yield relative to tetraploid ‘Trailhead’, octoploid ‘Magnar’ and interploidy-hybrid ‘Continental’ BWR cultivars in comparison with other perennial grasses across diverse single-harvest dryland range sites and a two-harvest irrigated production system. Two half-sib hybrid populations were produced by harvesting seed from the tetraploid self-incompatible Acc:641.T CWR genet, which was clonally propagated by rhizomes into isolated hybridization blocks with two tetraploid BWR pollen parents: Acc:636 and ‘Trailhead’. Full-sib hybrid seed was also produced from a controlled cross of tetraploid ‘Rio’ CWR and ‘Trailhead’ BWR plants. In space-planted range plots, the ‘Rio’ CWR × ‘Trailhead’ BWR and Acc:641.T CWR × Acc:636 BWR hybrids displayed high-parent heterosis with 75% and 36% yield advantages, respectively, but the Acc:641.T CWR × ‘Trailhead’ BWR hybrid yielded significantly less than its BWR high-parent in this evaluation. Half-sib CWR × BWR hybrids of Acc:636 and ‘Trailhead’ both yielded as good as or better than available BWR cultivars, with yields similar to switchgrass (Panicum virgatum, in the irrigated sward plots. These results elucidate opportunity to harness genetic variation among native grass species for the development of forage and bioenergy feedstocks in western North America.

  6. Floristic composition and weed biomass in organic alfalfa crop

    Directory of Open Access Journals (Sweden)

    Ilić Olivera M.

    2016-01-01

    Full Text Available The paper presents the results of floristic composition and weed biomass in organic alfalfa crop in different years of alfalfa growing. The experiment was conducted in experimental plots in Futog, during the vegetation period of 2015. The presence of 60 weed species was noted, 49 of all weeds were noticed in the third, 48 in the fourth and 34 in the fifth year of organic alfalfa growing. Quantitative and qualitative structure of weed community depended on the age of alfalfa crop. By the analysis of cover values and the degree of the presence of weed species, it can be seen that species: Rumex obtusifolius L., Taraxacum officinale Web., Cynodon dactylon Pers. and Artemisia vulgaris L. had the greatest significance on weed infestation of alfalfa. The largest number of weed species belonged to the families Poaceae and Asteraceae. The biological spectrum of weeds species was defined by the terophyta dominance, whose number was reduced due to the age of the crop, while the share of hemicryptophytes and geophytes was more or less constant. Although we found a considerable level of infestation of alfalfa, alfalfa biomass reached acceptable values that were significantly higher than the weed biomass, which was statistically highly significant.

  7. Micromonospora from nitrogen fixing nodules of alfalfa (Medicago sativa L.). A new promising Plant Probiotic Bacteria.

    OpenAIRE

    Pilar Martínez-Hidalgo; Purificación Galindo-Villardón; Trujillo, Martha E.; Igual, José M.; Eustoquio Martínez-Molina

    2014-01-01

    Biotic interactions can improve agricultural productivity without costly and environmentally challenging inputs. Micromonospora strains have recently been reported as natural endophytes of legume nodules but their significance for plant development and productivity has not yet been established. The aim of this study was to determine the diversity and function of Micromonospora isolated from Medicago sativa root nodules. Micromonospora-like strains from field alfalfa nodules were characteri...

  8. Clustering of HI galaxies in HIPASS and ALFALFA

    CERN Document Server

    Passmoor, S S; Faltenbacher, A

    2011-01-01

    We investigate the clustering of HI-selected galaxies in the ALFALFA survey and compare results with those obtained for HIPASS. Measurements of the angular correlation function and the inferred 3D-clustering are compared with results from direct spatial-correlation measurements. We are able to measure clustering on smaller angular scales and for galaxies with lower HI masses than was previously possible. We calculate the expected clustering of dark matter using the redshift distributions of HIPASS and ALFALFA and show that the ALFALFA sample is somewhat more anti-biased with respect to dark matter than the HIPASS sample.

  9. VARIABILIDAD DEL ECOTIPO DE ALFALFA 'TIERRA DE CAMPOS'

    OpenAIRE

    Fombellida Villafruela, A.

    2011-01-01

    La alfalfa es el cultivo forrajero más extendido en la comarca de Tierra de Campos. Se cultiva habitualmente en secano, con aprovechamiento mediante siega para heno y mediante pastoreo de los rebrotes, destinándose parte de superficie a la producción de semilla para uso propio. Esta forma de manejo, en este ambiente, a lo largo de los años, ha originado un tipo de alfalfa conocido con el nombre de 'Tierra de Campos'. La introducción en la comarca de alfalfas flamencas para su multiplicación, ...

  10. [Efficiency of alfalfa and reed in the phytoremediation of hydrocarbon polluted soil].

    Science.gov (United States)

    Muratova, A Iu; Turkovskaia, O V; Hubner, T; Kuschk, P

    2003-01-01

    The efficacy of plants as means of decontaminating hydrocarbon-polluted soil has been studied. Ditch reed (Phragmites australis) and alfalfa (Medicago sativa) markedly intensified processes of pollutant destruction, the effect being particularly pronounced in the case of polycyclic aromatic hydrocarbons (PAHs). Comparative analysis of microflora in soils (including those devoid of plants and rhizosphere) demonstrated that, in addition to preventing the pollutant-induced decrease in the amount of heterotrophic microorganisms, the plants stimulated their development, significantly increasing the population of destructors. Effects of plants on major physiological groups of soil microorganisms under conditions of pollution were ambiguous. The rhizosphere consortium of alfalfa was less susceptible to effects of pollutants than that of reed.

  11. Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region

    Directory of Open Access Journals (Sweden)

    Mohammed A. AL-Saleh

    2013-12-01

    Full Text Available In 2011–2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in Riyadh region. Mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for Alfalfa mosaic virus (AMV by ELISA were produced systemic mosaic on Vigna unguiculata and Nicotiana tabacum, local lesion on Chenopodium amaranticolor and C. quinoa. Vicia faba indicator plants that induce mosaic and mottle with AMV-Sagir isolate and no infection with AMV-Wadi aldawasser isolate. Approximately 700-bp was formed by RT-PCR using AMV coat protein specific primer. Samples from infected alfalfa gave positive results, while healthy plant gave negative result using dot blot hybridization assay. The nucleotide sequences of the Saudi isolates were compared with corresponding viral nucleotide sequences reported in GenBank. The obtained results showed that the AMV from Australia, Brazil, Puglia and China had the highest similarity with AMV-Sajer isolate. While, the AMV from Spain and New Zealaland had the lowest similarity with AMV-Sajer and Wadi aldawasser isolates. The data obtained in this study has been deposited in the GenBank under the accession numbers KC434083 and KC434084 for AMV-Sajer and AMV- Wadialdawasser respectively. This is the first report regarding the gnetic make up of AMV in Saudi Arabia.

  12. Chemical characterization of ash from gasification of alfalfa stems: Implications for ash management

    Energy Technology Data Exchange (ETDEWEB)

    Mozaffari, M.; Rosen, C.J.; Russelle, M.P.; Nater, E.A.

    2000-06-01

    Electricity generation from biomass is an attractive option from an environmental perspective. Pilot studies have indicated that alfalfa (Medicago sativa L.) stems are suitable feedstock for energy generation via gasification. Detailed information on chemical characteristics of the ash generated from gasification of alfalfa stem is required to develop environmentally and economically sound ash management strategies. Alfalfa fly and bottom ashes were characterized with respect to chemical properties that are important in developing ash management practices with emphasis on beneficial utilization as a soil amendment. Mean concentrations of total C, K, Ca, and Cl were 424, 120, 85, and 26 g kg{sup {minus}1}, respectively, in fly ash. In bottom ash, the mean concentrations of C, K, and Ca, were 63, 61, and 193 g kg{sup {minus}1}. Concentrations of total Pb, As, Cd, Co, and Se were below detection limits in both ash types. Naphthalene ranged from 6.2 to 74 mg kg{sup {minus}1}, but concentrations of many other polyaromatic hydrocarbons were low or below mg kg{sup {minus}1} detection limits. Available K and P in fly ash were 90 to 120 and 8 to 10 g kg{sup {minus}1}, respectively. Mean CaCO{sub 3} equivalent value of fly ash was 400 g kg{sup {minus}1}, its electrical conductivity (EC) and pH were 127 dS m{sup {minus}1} and 11.5, respectively. These results suggest that when managed properly, gasified alfalfa ash could potentially be utilized as a beneficial soil amendment with few potential environmental concerns.

  13. Global regulation of reactive oxygen species scavenging genes in alfalfa root and shoot under gradual drought stress and recovery.

    Science.gov (United States)

    Kang, Yun; Udvardi, Michael

    2012-05-01

    Reactive oxygen species (ROS) production and scavenging in plants under drought stress have been studied intensively in recent years. Here we report a global analysis of gene expression for the major ROS generating and scavenging proteins in alfalfa root and shoot under gradual drought stress followed by one-day recovery. Data from two alfalfa varieties, one drought tolerant and one drought sensitive, were compared and no qualitative differences in ROS gene regulation between the two were found. Conserved, tissue-specific patterns of gene expression in response to drought were observed for several ROS-scavenging gene families, including ascorbate peroxidase, monodehydroascorbate reductase, and peroxiredoxin. In addition, differential gene expression within families was observed. Genes for the ROS-generating enzyme, NADPH oxidase were generally induced under drought, while those for glycolate oxidase were repressed. Among the ROS-scavenging protein genes, Ferritin, Cu/Zn superoxide dismutase (SOD), and the majority of the glutathione peroxidase family members were induced under drought in both roots and shoots of both alfalfa varieties. In contrast, Fe-SOD, CC-type glutaredoxins, and thoiredoxins were downregulated.

  14. Observaciones sobre el Cultivo de la Alfalfa

    Directory of Open Access Journals (Sweden)

    Mejía. Pedro Nel

    1947-09-01

    Full Text Available En 1940, la Secretaría de Agricultura de Caldas estableció en la fracción de la Enea del Municipio de Manizales un pequeño predio experimental o campo de observación para forrajes, destinado al fomento y divulgación de los mismos. Los datos que consignamos en el presente estudio se refiere a los retados experimentales del lote de alfalfa N° 3 de la granja, que está compuesto por una área de 1947 metros cuadrados. Sirve esto para que las personas interesadas hagan la reducción a la plaza (6.400 metros cuadrados y a la hectárea (10.000 metros cuadrados.

  15. EFFECT OF STORAGE TIME ON ALFALFA SEED QUALITY

    Directory of Open Access Journals (Sweden)

    Tihomir ČUPIĆ

    2005-07-01

    Full Text Available Alfalfa (Medicago sativa L. is a crop characterised by presence of hard (dormant seed that are viable but do not germinate in seed quality testing. In Republic of Croatia seed are frequently stored for three to four years due to low needs for seed of alfalfa and considerable import. The share of hard seed is decreased by storage time, temperature and air moisture changes and therefore directly keeps the level of seed germination in a longer period of storage.

  16. Multiwalled carbon nanotubes in alfalfa and wheat: toxicology and uptake

    OpenAIRE

    Miralles, Pola; Johnson, Errin; Tamara L. Church; Harris, Andrew T.

    2012-01-01

    Data on the bioavailability and toxicity of carbon nanotubes (CNTs) in the environment, and, in particular, on their interactions with vascular plants, are limited. We investigated the effects of industrial-grade multiwalled CNTs (75 wt% CNTs) and their impurities on alfalfa and wheat. Phytotoxicity assays were performed during both seed germination and seedling growth. The germinations of both species were tolerant of up to 2560 mg l−1 CNTs, and root elongation was enhanced in alfalfa and wh...

  17. Economic Comparison of Selected Processing Alternatives for Alfalfa

    OpenAIRE

    Bates, Dan J.

    1992-01-01

    Processing alfalfa for export is of significant interest to areas like Millard County, the largest hay-producing county in Utah. In the past year there have ix been significant reductions in the price of hay as a result of increased supplies in the central and western United States. This thesis analyzes the benefits and costs of processing alfalfa into cubes and recompressed bales in order to enter the export market. Costs of production were estimated through the use of enterprise budgets ...

  18. Polar varieties revisited

    OpenAIRE

    Piene, Ragni

    2016-01-01

    We recall the definition of classical polar varieties, as well as those of affine and projective reciprocal polar varieties. The latter are defined with respect to a non-degenerate quadric, which gives us a notion of orthogonality. In particular we relate the reciprocal polar varieties to the "Euclidean geometry" in projective space. The Euclidean distance degree and the degree of the focal loci can be expressed in terms of the ranks, i.e., the degrees of the classical polar varieties, and he...

  19. Differential antifungal activity of alfalfa (Medicago santva L. saponins originated from roots and aerial parts for some ornamental plant pathogens

    Directory of Open Access Journals (Sweden)

    Alicja Saniewska

    2013-12-01

    Full Text Available The total saponins isolated from aerial parts and roots of alfalfa (Medicago sativa L. at the concentration of 0,01%, 0,05% and 0,1% showed differential influence on the mycelium growth of Alternaria zinniae, Botrytis cinerea, Botrytis tulipae, Phoma narcissi, Phoma poolensis and Rhizoctonia solani. A higher inhibitory effect on in vitro growth of mycelium of all tested pathogens indicated saponins from roots of alfalfa than from aerial parts. Tested saponins from roots at lhe concentration of 0,1% totally inhibited linear growth of Phoma narcissi, and linear growth of Alternaria zinniae was limited about 67%, Botrytis cinerea about 74%, Botrytis tulipae about 68%, Phoma poolensis about 38%, and Rhizoctonia solani about 74% in comparison to the control. The saponins of alfalfa from roots at the concentration of 0,1% and 0,2% applied as preinoculation sprays evidently inhibited the development of Phoma narcissi on Hippeastrum leaves. This dose of aerial saponins of alfalfa did not effect the development of necrotic spots on the leaves.

  20. Micromonospora from nitrogen fixing nodules of alfalfa (Medicago sativa L.). A new promising Plant Probiotic Bacteria.

    Science.gov (United States)

    Martínez-Hidalgo, Pilar; Galindo-Villardón, Purificación; Trujillo, Martha E; Igual, José M; Martínez-Molina, Eustoquio

    2014-09-17

    Biotic interactions can improve agricultural productivity without costly and environmentally challenging inputs. Micromonospora strains have recently been reported as natural endophytes of legume nodules but their significance for plant development and productivity has not yet been established. The aim of this study was to determine the diversity and function of Micromonospora isolated from Medicago sativa root nodules. Micromonospora-like strains from field alfalfa nodules were characterized by BOX-PCR fingerprinting and 16S rRNA gene sequencing. The ecological role of the interaction of the 15 selected representative Micromonospora strains was tested in M. sativa. Nodulation, plant growth and nutrition parameters were analyzed. Alfalfa nodules naturally contain abundant and highly diverse populations of Micromonospora, both at the intra- and at interspecific level. Selected Micromonospora isolates significantly increase the nodulation of alfalfa by Ensifer meliloti 1021 and also the efficiency of the plant for nitrogen nutrition. Moreover, they promote aerial growth, the shoot-to-root ratio, and raise the level of essential nutrients. Our results indicate that Micromonospora acts as a Rhizobia Helper Bacteria (RHB) agent and has probiotic effects, promoting plant growth and increasing nutrition efficiency. Its ecological role, biotechnological potential and advantages as a plant probiotic bacterium (PPB) are also discussed.

  1. Partitioning and mobilization of photoassimilate in alfalfa subjected to water deficits. [Medicago sativa L

    Energy Technology Data Exchange (ETDEWEB)

    Hall, M.H. (Univ. of Idaho, Moscow (USA)); Sheaffer, C.C.; Heichel, G.H. (Univ. of Minnesota, St. Paul (USA))

    Faster regrowth of a stressed alfalfa (Medicago sativa L.) crop compared to an unstressed crop after rewatering has been reported. The bases of this compensatory response are unknown, but they may be important to understanding adaptation to water stress and to developing crop water management strategies. The authors objectives was to determine the effect of stress induced by water deficit on photoassimilate partitioning and the utilization of stored assimilates during regrowth of alfalfa. Field and greenhouse experiments were conducted using cultivars differing in winterhardiness. Plants were subjected to water stress, pulse-labeled with {sup 14}CO{sub 2}, and sampled following 0, 1, 14, 21, and 28-d translocation periods. Following the 14-d sampling, herbage was harvested and water stress was removed. Cultivars contrasting in winterhardiness responded similarly to water stress. Stressed plant roots contained 73 and 114% more total plant radioactivity (TPR) than the control at the 1 and 14-d translocation periods, respectively. Water stress significantly increased root starch and TPR percentage in the starch fraction, but had much smaller effects on root soluble-sugar concentration and TPR percentage of the root sugar fraction. Herbage regrowth mass following harvest and rewatering of the water-stressed plants was similar to that of the control. Compared to the control, water-stressed alfalfa has greater total nonstructural carbohydrates in the roots, apparently due to increased photoassimilate partitioning to the roots. However, the greater root carbohydrate concentrations did not result in compensatory herbage regrowth following rewatering.

  2. Computing Tropical Varieties

    DEFF Research Database (Denmark)

    Speyer, D.; Jensen, Anders Nedergaard; Bogart, T.;

    2005-01-01

    The tropical variety of a d-dimensional prime ideal in a polynomial ring with complex coefficients is a pure d-dimensional polyhedral fan. This fan is shown to be connected in codimension one. We present algorithmic tools for computing the tropical variety, and we discuss our implementation...

  3. REAL PIECEWISE ALGEBRAIC VARIETY

    Institute of Scientific and Technical Information of China (English)

    Ren-hong Wang; Yi-sheng Lai

    2003-01-01

    We give definitions of real piecewise algebraic variety and its dimension. By using the techniques of real radical ideal, P-radical ideal, affine Hilbert polynomial, Bernstein-net form of polynomials on simplex, and decomposition of semi-algebraic set, etc., we deal with the dimension of the real piecewise algebraic variety and real Nullstellensatz in Cμ spline ring.

  4. Identification of Loci Associated with Drought Resistance Traits in Heterozygous Autotetraploid Alfalfa (Medicago sativa L. Using Genome-Wide Association Studies with Genotyping by Sequencing.

    Directory of Open Access Journals (Sweden)

    Tiejun Zhang

    Full Text Available Drought resistance is an important breeding target for enhancing alfalfa productivity in arid and semi-arid regions. Identification of genes involved in drought tolerance will facilitate breeding for improving drought resistance and water use efficiency in alfalfa. Our objective was to use a diversity panel of alfalfa accessions comprised of 198 cultivars and landraces to identify genes involved in drought tolerance. The panel was selected from the USDA-ARS National Plant Germplasm System alfalfa collection and genotyped using genotyping by sequencing. A greenhouse procedure was used for phenotyping two important traits associated with drought tolerance: drought resistance index (DRI and relative leaf water content (RWC. Marker-trait association identified nineteen and fifteen loci associated with DRI and RWC, respectively. Alignments of target sequences flanking to the resistance loci against the reference genome of M. truncatula revealed multiple chromosomal locations. Markers associated with DRI are located on all chromosomes while markers associated with RWC are located on chromosomes 1, 2, 3, 4, 5, 6 and 7. Co-localizations of significant markers between DRI and RWC were found on chromosomes 3, 5 and 7. Most loci associated with DRI in this work overlap with the reported QTLs associated with biomass under drought in alfalfa. Additional significant markers were targeted to several contigs with unknown chromosomal locations. BLAST search using their flanking sequences revealed homology to several annotated genes with functions in stress tolerance. With further validation, these markers may be used for marker-assisted breeding new alfalfa varieties with drought resistance and enhanced water use efficiency.

  5. Identification of Loci Associated with Drought Resistance Traits in Heterozygous Autotetraploid Alfalfa (Medicago sativa L.) Using Genome-Wide Association Studies with Genotyping by Sequencing.

    Science.gov (United States)

    Zhang, Tiejun; Yu, Long-Xi; Zheng, Ping; Li, Yajun; Rivera, Martha; Main, Dorrie; Greene, Stephanie L

    2015-01-01

    Drought resistance is an important breeding target for enhancing alfalfa productivity in arid and semi-arid regions. Identification of genes involved in drought tolerance will facilitate breeding for improving drought resistance and water use efficiency in alfalfa. Our objective was to use a diversity panel of alfalfa accessions comprised of 198 cultivars and landraces to identify genes involved in drought tolerance. The panel was selected from the USDA-ARS National Plant Germplasm System alfalfa collection and genotyped using genotyping by sequencing. A greenhouse procedure was used for phenotyping two important traits associated with drought tolerance: drought resistance index (DRI) and relative leaf water content (RWC). Marker-trait association identified nineteen and fifteen loci associated with DRI and RWC, respectively. Alignments of target sequences flanking to the resistance loci against the reference genome of M. truncatula revealed multiple chromosomal locations. Markers associated with DRI are located on all chromosomes while markers associated with RWC are located on chromosomes 1, 2, 3, 4, 5, 6 and 7. Co-localizations of significant markers between DRI and RWC were found on chromosomes 3, 5 and 7. Most loci associated with DRI in this work overlap with the reported QTLs associated with biomass under drought in alfalfa. Additional significant markers were targeted to several contigs with unknown chromosomal locations. BLAST search using their flanking sequences revealed homology to several annotated genes with functions in stress tolerance. With further validation, these markers may be used for marker-assisted breeding new alfalfa varieties with drought resistance and enhanced water use efficiency.

  6. Detection and quantification of additives (urea, biuret and poultry litter) in alfalfas by NIR spectroscopy with fibre-optic probe.

    Science.gov (United States)

    González-Martín, Inmaculada; Hernández-Hierro, José Miguel

    2008-09-15

    The additives (urea, biuret and poultry litter) present in alfalfa, which contribute non-proteic nitrogen, were analysed using near infrared spectroscopy (NIRS) technology together with a remote reflectance fibre-optic probe. We used 75 samples of known alfalfa without additives and 75 samples with each of the additives, urea (0.01-10%), biuret (0.01-10%) and poultry litter (1-25%). Using the discriminant partial least squares (DPLS) algorithm, the presence or absence of the additives urea, biuret and poultry litter is classified and predicted with a high prediction rate of 96.9%, 100% and 100%, obtaining the equations of discrimination for each additive. The regression method employed for the quantification was modified partial least squares (MPLS). The equations were developed using the fibre-optic probe to determine the content of urea, biuret and poultry litter with multiple correlation coefficients (RSQ) and prediction corrected standard errors (SEP (C)) of 0.990, 0.28% for urea, 0.991, 0.29% for biuret and 0.925, 2.08% for poultry litter. The work permits the instantaneous and simultaneous prediction and determination of urea, biuret and poultry litter in alfalfas, applying the fibre-optic directly on the ground samples of alfalfa.

  7. Transcript analysis in two alfalfa salt tolerance selected breeding populations relative to a non-tolerant population.

    Science.gov (United States)

    Gruber, M Y; Xia, J; Yu, M; Steppuhn, H; Wall, K; Messer, D; Sharpe, A G; Acharya, S N; Wishart, D S; Johnson, D; Miller, D R; Taheri, A

    2017-02-01

    With the growing limitations on arable land, alfalfa (a widely cultivated, low-input forage) is now being selected to extend cultivation into saline lands for low-cost biofeedstock purposes. Here, minerals and transcriptome profiles were compared between two new salinity-tolerant North American alfalfa breeding populations and a more salinity-sensitive western Canadian alfalfa population grown under hydroponic saline conditions. All three populations accumulated two-fold higher sodium in roots than shoots as a function of increased electrical conductivity. At least 50% of differentially expressed genes (p population growing under high salinity, while expression remained unchanged in the saline-tolerant populations. In particular, most reduction in transcript levels in the salt-sensitive population was observed in genes specifying cell wall structural components, lipids, secondary metabolism, auxin and ethylene hormones, development, transport, signalling, heat shock, proteolysis, pathogenesis-response, abiotic stress, RNA processing, and protein metabolism. Transcript diversity for transcription factors, protein modification, and protein degradation genes was also more strongly affected in salt-tolerant CW064027 than in salt-tolerant Bridgeview and salt-sensitive Rangelander, while both saline-tolerant populations showed more substantial up-regulation in redox-related genes and B-ZIP transcripts. The report highlights the first use of bulked genotypes as replicated samples to compare the transcriptomes of obligate out-cross breeding populations in alfalfa.

  8. Complete nucleotide sequence of Alfalfa mosaic virus isolated from alfalfa (Medicago sativa L.) in Argentina.

    Science.gov (United States)

    Trucco, Verónica; de Breuil, Soledad; Bejerman, Nicolás; Lenardon, Sergio; Giolitti, Fabián

    2014-06-01

    The complete nucleotide sequence of an Alfalfa mosaic virus (AMV) isolate infecting alfalfa (Medicago sativa L.) in Argentina, AMV-Arg, was determined. The virus genome has the typical organization described for AMV, and comprises 3,643, 2,593, and 2,038 nucleotides for RNA1, 2 and 3, respectively. The whole genome sequence and each encoding region were compared with those of other four isolates that have been completely sequenced from China, Italy, Spain and USA. The nucleotide identity percentages ranged from 95.9 to 99.1 % for the three RNAs and from 93.7 to 99 % for the protein 1 (P1), protein 2 (P2), movement protein and coat protein (CP) encoding regions, whereas the amino acid identity percentages of these proteins ranged from 93.4 to 99.5 %, the lowest value corresponding to P2. CP sequences of AMV-Arg were compared with those of other 25 available isolates, and the phylogenetic analysis based on the CP gene was carried out. The highest percentage of nucleotide sequence identity of the CP gene was 98.3 % with a Chinese isolate and 98.6 % at the amino acid level with four isolates, two from Italy, one from Brazil and the remaining one from China. The phylogenetic analysis showed that AMV-Arg is closely related to subgroup I of AMV isolates. To our knowledge, this is the first report of a complete nucleotide sequence of AMV from South America and the first worldwide report of complete nucleotide sequence of AMV isolated from alfalfa as natural host.

  9. Evaluation of Selection Indices for Alfalfa (Medicago sativa L.

    Directory of Open Access Journals (Sweden)

    Hassan MONIRIFAR

    2010-03-01

    Full Text Available In order to evaluate the efficiency of selection indices in alfalfa improvement, an experiment was conducted from 2000 to 2007 at East Azarbaijan Agricultural and Natural Resources Research Center, Iran. A set of 29 native ecotypes, which were collected in 2000 and 2001 from Azerbaijan (Iran, together with one improved variety were used to conduct a polycross nursery. For the purpose of random mating, a randomized complete block design was used with 12 replications arranged in this nursery. The resulting 30 half-sib families were planted individually in pots and the 30 days old seedlings were transplanted to the field in May, 2004. Each 3-row plot consisted of 45 HS plants. Several traits, such as individual plants� fresh weight and individual plants� dry weight (IPFW, IPDW, number of shoots per plant (NS and plant height (PH in each harvest and also, days until 10% flowering, the ratio of fresh and dry weight of leaves/plant and size of trifoliate leaves were measured for three cropping seasons. The results of analysis of variance showed large variation among polycross progenies. Six selection indices (Ii with different number of traits at adult plant stage were evaluated. Based on the result of this investigation, if number of shoots and height of adult plant, excluding yield, are recorded, I2 is suggested. If, in addition to fresh yield, height of adult plant is measured, I6 is recommended. I4 is useful when number of shoots and plant height with dry yield are included in the index. In conclusion, the importance of mature plant traits in selection indices was in the order of yield > plant height > number of shoots. The results provided more evidence that selection indices incorporating the component of dry yield are more advantageous. The most efficient selection index consisted of NS, IPDW and PH, having a relative efficiency of 280%.

  10. Stem nematode counteracts plant resistance of aphids in alfalfa, Medicago sativa.

    Science.gov (United States)

    Ramirez, Ricardo A; Spears, Lori R

    2014-10-01

    Plants are exploited by a diverse community of insect herbivores and phytopathogens that interact indirectly through plant-mediated interactions. Generally, plants are thought to respond to insects and pathogens through different defensive signaling pathways. As plants are selected for resistance to one phytophagous organism type (insect vs. pathogen) in managed systems, it is not clear how this selection may affect community interactions. This study examined the effect of nematode-resistant varieties on aphid (Acyrthosiphon pisum) suppression, and then determined how infection by the stem nematode, Ditylenchus dipsaci, mediated ecological effects on aphids and on plant defense proteins. Four alfalfa (Medicago sativa) varieties were selected with resistance to nematodes only (+,-), aphids only (-,+), nematodes and aphids (+,+), and susceptibility to nematodes and aphids (-,-). Field and greenhouse experiments were conducted to isolate the effect of nematode infection and aphid abundance on each variety. We found that varieties resistant to nematode, regardless of aphid resistance, had the lowest aphid counts, suggesting possible cross-resistance. Aphid abundance, however, increased when plants were exposed to nematodes. Resistant varieties were associated with elevated saponins but these compounds were not affected by insect or pathogen feeding. Concentrations of peroxidases and trypsin inhibitors, however, were increased in nematode resistant varieties when exposed to nematodes and aphids, respectively. The patterns of plant defense were variable, and a combination of resistance traits and changes in nutrient availability may drive positive interactions between nematodes and aphids aboveground.

  11. Development of Watermelon Variety —— 'Heitong'%高产优质西瓜新品种黑彤的选育

    Institute of Scientific and Technical Information of China (English)

    胡安杰; 史宣杰; 凌华; 蔡毓新; 高冠英; 师恭耀

    2009-01-01

    Watermelon variety Heitong was developed by crossing HY-18 as female parent and LC-6 as male parent. It is a high yield variety with excellent quality,vigorous plant and easy fruit-set. The total growing period is about 105 days and the fruit matures about 34 days after pollination. The fruit is oval-shaped with fruit shape index of 1.40. The skin color is dark green skin without stripes. The rind thickness is 1.2 cm and is suitable for long distance shipping. The average fruit weight is about 6 kg,and the yield on 667 m2 is 3 000-4 000 kg in open field. The flesh is red with fine crisp texture and sweet flavor. The center sugar content is over 12.5%. It has good adaptability and can be grown in most watermelon production areas in China. It was officially approved for registration by Henan Commercial Variety Registation Authority in 2008.%黑彤西瓜是以HY-18为母本,LC-6为父本配制而成的中晚熟、高产、优质、大果型黑皮西瓜新品种.植株生长健壮,易坐果.全生育期105 d左右,果实发育期34d左右.果实椭圆形,果形指数1.4,黑皮,外形美观;果皮厚1.2 cm.硬韧,耐贮运.单果质量6 kg左右,最大可达12kg左右,普通栽培667m2产量3000-4000 kg.鲜红瓤,瓤质硬脆,少籽,中心可溶性固形物11%左右,边部9%左右,品质优良.适应性广,全国各地均可栽培.2008年通过河南省农作物品种审定委员会审定,审定编号:豫审西瓜2008011.

  12. Effects of AN Alfalfa (medicago Sativa) Buffer Strip on Leached δ15NNITRATE Values: Implications for Management of Hydrologic N Losses

    Science.gov (United States)

    Kelley, C. J.; Keller, C. K.; Smith, J. L.; Evans, R. D.; Harlow, B.

    2011-12-01

    Buffer strips are commonly used to decrease agricultural runoff with the objective of limiting sediment and agrochemicals fluxes to surface waters. The objective of this study was to determine the effects of an alfalfa buffer strip on the magnitude and source(s) of leached nitrate from a dryland agricultural field. Previous research at the Cook Agronomy Farm has inferred two sources of nitrate in tile drain discharge, a high-discharge-season (January through May) synthetic fertilizer source, and a low-discharge-season (June through December) soil organic nitrogen source. This study examines how a change in management strategy and crop species alters the low discharge season nitrate source. In the spring of 2006 an alfalfa buffer strip approximately 20 m wide was planted running approximately north-south in the lowland portion of a 12 ha tile-drained field bordering a ditch that drains into Missouri Flat Creek. Three-year (2003 through 2005) average NO3--N flux prior to the planting of the alfalfa buffer strip was ~0.40 kg ha-1 year-1. After planting, the three-year (2006 through 2008) average NO3--N flux was ~0.38 kg ha-1 year-1. The lack of evident buffer-strip influence on the fluxes may be due in part to the large variation in precipitation amounts and timing that control water flows through the system. Three-year average δ15Nnitrate values for the tile drain pre and post planting of the alfalfa buffer strip were 6.9 ± 1.1 % and 4.2 ± 0.9 % respectively. We hypothesize that the significant difference indicates that the alfalfa strip affects the source of leached nitrate. Before planting the alfalfa buffer strip, the interpreted source of nitrate was mineralization of soil organic nitrogen from non-N2 fixing crops (spring and summer wheat varieties). After planting the alfalfa buffer strip, the source of nitrate was interpreted to be a mixture of mineralized soil organic nitrogen from N2 fixing alfalfa and non-N2 fixing crops. Further work is needed to test

  13. Identification of transcriptome involved in atrazine detoxification and degradation in alfalfa (Medicago sativa) exposed to realistic environmental contamination.

    Science.gov (United States)

    Zhang, Jing Jing; Lu, Yi Chen; Zhang, Shu Hao; Lu, Feng Fan; Yang, Hong

    2016-08-01

    Plants are constantly exposed to a variety of toxic compounds (or xenobiotics) such as pesticides (or herbicides). Atrazine (ATZ) as herbicide has become one of the environmental contaminants due to its intensive use during crop production. Plants have evolved strategies to cope with the adverse impact of ATZ. However, the mechanism for ATZ degradation and detoxification in plants is largely unknown. Here we employed a global RNA-sequencing (RNA-Seq) strategy to dissect transcriptome variation in alfalfa (Medicago sativa) exposed to ATZ. Four libraries were constructed including Root-ATZ (root control, ATZ-free), Shoot-ATZ, Root+ATZ (root treated with ATZ) and Shoot+ATZ. Hierarchical clustering was performed to display the expression patterns for all differentially expressed genes (DEGs) under ATZ exposure. Transcripts involved in ATZ detoxification, stress responses (e.g. oxidation and reduction, conjugation and hydrolytic reactions), and regulations of cysteine biosynthesis were identified. Several genes encoding glycosyltransferases, glutathione S-transferases or ABC transporters were up-regulated notably. Also, many other genes involved in oxidation-reduction, conjugation, and hydrolysis for herbicide degradation were differentially expressed. These results suggest that ATZ in alfalfa can be detoxified or degraded through different pathways. The expression patterns of some DEGs by high-throughput sequencing were well confirmed by qRT-PCR. Our results not only highlight the transcriptional complexity in alfalfa exposed to ATZ but represent a major improvement for analyzing transcriptional changes on a large scale as well.

  14. Genomic Analysis of Verticillium Wilt Resistance and Drought Tolerance in Alfalfa

    Science.gov (United States)

    Alfalfa (Medicago sativa L.) is the fourth largest crop in the United States. Changing trends to multipurpose uses increases demand for alfalfa. However, the production of alfalfa is challenged by endemic and emerging diseases and adverse environmental factors. Identification of genes/loci controlli...

  15. Stand age affects fertilizer nitrogen response in first-year corn following alfalfa

    Science.gov (United States)

    The amount of N that alfalfa (Medicago sativa L.) provides to subsequent first-year corn (Zea mays L.) depends, in part, on the age of alfalfa at termination. Our objective was to determine how alfalfa stand age affects N availability and fertilizer N requirements for first-year corn. Fertilizer N w...

  16. Modeling feral alfalfa (Medicago sativa subsp. sativa L.) occurrence using topographical and environmental variables

    Science.gov (United States)

    Because alfalfa is a perennial species cross pollinated by bees and can establish along roadsides and ruderal areas, there is concern that feral plants can serve as reservoirs and conduits for transgenic genes. The objective of this study was to survey feral alfalfa in alfalfa seed production areas ...

  17. Enhancing pollination by attracting & retaining leaf cutting bees (Megachile rotundata) in alfalfa seed production fields

    Science.gov (United States)

    The alfalfa leafcutting bee, Megachile rotundata (F.), has become an important managed pollinator of alfalfa, Medicago sativa L. One problem when using alfalfa leafcutting bees as managed pollinator, is the dispersal of many females upon release, even when adequate nesting sites are present. While d...

  18. 75 FR 8299 - Draft Environmental Impact Statement; Determination of Regulated Status of Alfalfa Genetically...

    Science.gov (United States)

    2010-02-24

    ... Regulated Status of Alfalfa Genetically Engineered for Tolerance to the Herbicide Glyphosate AGENCY: Animal... and Forage Genetics International alfalfa lines designated as events J101 and J163 as regulated... determination on the status of the Monsanto Company and Forage Genetics International alfalfa lines...

  19. Pythium species causing damping-off of alfalfa in Minnesota: Identification, pathogenicity and fungicide sensitivity

    Science.gov (United States)

    Damping-off and seed rot is an important disease of alfalfa, severely affecting stand establishment when conditions favor the disease. Globally, 15 Pythium species are reported to cause damping-off and seed rot of alfalfa, although surveys of species causing disease on alfalfa in Minnesota are lacki...

  20. Do glyphosate resistant feral plants and hay fields spread the transgene to conventional alfalfa seed fields?

    Science.gov (United States)

    In addition to meeting domestic needs, large amounts of alfalfa seed and hay produced in the US are being exported overseas. Because alfalfa is an insect pollinated crop, gene flow is a concern. Adding to this alfalfa readily naturalizes along roadsides, irrigation ditches, and unmanaged habitats; a...

  1. Transgene movement in commercial alfalfa seed production: Implications for seed purity

    Science.gov (United States)

    The United States is a major exporter of alfalfa seed and hay and the organic dairy industry is one of the fastest growing agricultural sectors. With the advent of genetically-engineered (GE) alfalfa concerns have risen regarding the coexistence of GE and non GE alfalfa since the crop is largely ou...

  2. 76 FR 5780 - Determination of Regulated Status of Alfalfa Genetically Engineered for Tolerance to the...

    Science.gov (United States)

    2011-02-02

    ... Animal and Plant Health Inspection Service Determination of Regulated Status of Alfalfa Genetically... regulated status of alfalfa genetically engineered for tolerance to the herbicide glyphosate based on APHIS... decision and determination on the petition regarding the regulated status of alfalfa genetically...

  3. 75 FR 1585 - Draft Environmental Impact Statement; Determination of Regulated Status of Alfalfa Genetically...

    Science.gov (United States)

    2010-01-12

    ... Regulated Status of Alfalfa Genetically Engineered for Tolerance to the Herbicide Glyphosate AGENCY: Animal... determination on the status of the Monsanto Company and Forage Genetics International alfalfa lines designated... Monsanto/Forage Genetics International (FGI) alfalfa events J101 and J163 were no longer...

  4. Pollen and seed mediated gene flow in commercial alfalfa seed production fields

    Science.gov (United States)

    The potential for gene flow has been widely recognized since alfalfa is pollinated by bees. The Western US is a major exporter of alfalfa seed and hay and the organic dairy industry is one of the fastest growing agricultural sectors. Because of this, many alfalfa producers are impacted by market sen...

  5. Gas, Stars, and Star Formation in Alfalfa Dwarf Galaxies

    Science.gov (United States)

    Huang, Shan; Haynes, Martha P.; Giovanelli, Riccardo; Brinchmann, Jarle; Stierwalt, Sabrina; Neff, Susan G.

    2012-01-01

    We examine the global properties of the stellar and Hi components of 229 low H i mass dwarf galaxies extracted from the ALFALFA survey, including a complete sample of 176 galaxies with H i masses ALFALFA dwarfs are faint and of low surface brightness; only 56% of those within the SDSS footprint have a counterpart in the SDSS spectroscopic survey. A large fraction of the dwarfs have high specific star formation rates (SSFRs), and estimates of their SFRs and M* obtained by SED fitting are systematically smaller than ones derived via standard formulae assuming a constant SFR. The increased dispersion of the SSFR distribution at M* approximately less than10(exp 8)M(sub 0) is driven by a set of dwarf galaxies that have low gas fractions and SSFRs; some of these are dE/dSphs in the Virgo Cluster. The imposition of an upper Hi mass limit yields the selection of a sample with lower gas fractions for their M* than found for the overall ALFALFA population. Many of the ALFALFA dwarfs, particularly the Virgo members, have H i depletion timescales shorter than a Hubble time. An examination of the dwarf galaxies within the full ALFALFA population in the context of global star formation (SF) laws is consistent with the general assumptions that gas-rich galaxies have lower SF efficiencies than do optically selected populations and that Hi disks are more extended than stellar ones.

  6. Multiwalled carbon nanotubes in alfalfa and wheat: toxicology and uptake.

    Science.gov (United States)

    Miralles, Pola; Johnson, Errin; Church, Tamara L; Harris, Andrew T

    2012-12-07

    Data on the bioavailability and toxicity of carbon nanotubes (CNTs) in the environment, and, in particular, on their interactions with vascular plants, are limited. We investigated the effects of industrial-grade multiwalled CNTs (75 wt% CNTs) and their impurities on alfalfa and wheat. Phytotoxicity assays were performed during both seed germination and seedling growth. The germinations of both species were tolerant of up to 2560 mg l(-1) CNTs, and root elongation was enhanced in alfalfa and wheat seedlings exposed to CNTs. Remarkably, catalyst impurities also enhanced root elongation in alfalfa seedlings as well as wheat germination. Thus the impurities, not solely the CNTs, impacted the plants. CNT internalization by plants was investigated using electron microscopy and two-dimensional Raman mapping. The latter showed that CNTs were adsorbed onto the root surfaces of alfalfa and wheat without significant uptake or translocation. Electron microscopy investigations of internalization were inconclusive owing to poor contrast, so Fe(3)O(4)-functionalized CNTs were prepared and studied using energy-filter mapping of Fe(3)O(4). CNTs bearing Fe(3)O(4) nanoparticles were detected in the epidermis of one wheat root tip only, suggesting that internalization was possible but unusual. Thus, alfalfa and wheat tolerated high concentrations of industrial-grade multiwalled CNTs, which adsorbed onto their roots but were rarely taken up.

  7. INFLUENCES OF LIMING ON YIELDS OF ALFALFA HAY

    Directory of Open Access Journals (Sweden)

    Svetislav Popović

    2009-06-01

    Full Text Available Alfalfa is the most important forage legume on cultivated fields in Croatia (about 45000 ha of growing area – status 2003. The field experiment with application of four dolomite (Agrovapno MgO: 56% CaO and 40% MgO rates (0, 10, 20, 30 and 40 tha-1 were conducted in autumn of 2004. The experiment was conducted by randomized block design in four replicates. Alfalfa (cultivar Osječanka 88 of the Agricultural Institute Osijek, Croatia was sown on March 25, 2005. Four cuttings / year were made. Fresh mass of alfalfa (cutting area 0.25 m2 was oven-dried at 65 oC. Year was the most influencing factor of alfalfa hay yields (13.03, 28.63, 29.43 and 32.77 tha-1, for 2005, 2006, 2007 and 2008, respectively. Liming resulted in low increases of yields up to 5% only. We presume that possible high tolerance of Osječanka 88 cultivar to soil acidity could be the main reason of low effects of liming on alfalfa yields.

  8. Microsatellite genotyping of carnation varieties

    NARCIS (Netherlands)

    Smulders, M.J.M.; Noordijk, Y.; Rus-Kortekaas, W.; Bredemeijer, G.M.M.; Vosman, B.

    2003-01-01

    A set of 11 sequence-tagged microsatellite markers for carnation (Dianthus caryophyllus) was developed using a DNA library enriched for microsatellites. Supplemented with three markers derived from sequence database entries, these were used to genotype carnation varieties using a semi-automated fluo

  9. Multiple centroid method to evaluate the adaptability of alfalfa genotypes

    Directory of Open Access Journals (Sweden)

    Moysés Nascimento

    2015-02-01

    Full Text Available This study aimed to evaluate the efficiency of multiple centroids to study the adaptability of alfalfa genotypes (Medicago sativa L.. In this method, the genotypes are compared with ideotypes defined by the bissegmented regression model, according to the researcher's interest. Thus, genotype classification is carried out as determined by the objective of the researcher and the proposed recommendation strategy. Despite the great potential of the method, it needs to be evaluated under the biological context (with real data. In this context, we used data on the evaluation of dry matter production of 92 alfalfa cultivars, with 20 cuttings, from an experiment in randomized blocks with two repetitions carried out from November 2004 to June 2006. The multiple centroid method proved efficient for classifying alfalfa genotypes. Moreover, it showed no unambiguous indications and provided that ideotypes were defined according to the researcher's interest, facilitating data interpretation.

  10. Adaptability of Alfalfa and Ryegrass to New Reclamation Materials

    Institute of Scientific and Technical Information of China (English)

    WANG ZHAO-FENG; ZHANG Lei-na; FENG Yong-jun; KANG Jing-tao

    2006-01-01

    This paper discussed the adaptability of the alfalfa and ryegrass to the new artificial soil that was composed of fly ash and furfural residue. The potted experiments were taken to compare the growth conditions, yields, and qualities of both grasses. Some pots were covered with wheat stalks and others without. The medium Fluvo-aquic soil was taken as control. The results indicated that the growth of two grasses in the mixture were inferior to their growth in the control in initial period. With the leaching of rain and irrigation, the two grasses improved gradually. The alfalfa and ryegrass both could grow in the new mixture, and the ryegrass would be better to plant in no covered material and the alfalfa in covered. This will be a new way to reclaim subsided land and to improve the environment in mining area.

  11. Detection of the genetic variation of polygalacturonase-inhibiting protein gene 2 in autotetraploid alfalfa (Medicago sativa) using an improved SSCP technique.

    Science.gov (United States)

    Gui, Z; Liu, H Q; Wang, Y; Yuan, Q H; Xin, N; Zhang, X; Li, X L; Pi, Y S; Gao, J M

    2014-12-04

    In this study, 2 approaches were adopted to obtain good single-strand conformation polymorphism (SSCP) data for autotetraploid alfalfa; primers were added to PCR products, and fluorescent-labeled primers were utilized. PCR-SSCP conditions for a 331-bp fragment in the coding region of polygalacturonase-inhibiting protein gene 2 in alfalfa (MsPGIP2) were optimized, and the results showed that the best SSCP gel pattern could be obtained when the loading mixture was made by mixing 1 μL PCR products, 0.2 to 0.8 μL unlabeled primers (50 μM) and 4 to 16 μL loading buffer. Furthermore, the use of the fluorescent-labeled primers resulted in 2 separated electrophoresis images from 2 complementary single DNA strands, thus making the determination of alleles and idiotypes a relatively easy task. In addition, the results of sequencing prove that the determination of alleles and idiotypes were accurate based on SSCP analysis. Finally, a total of 9 alleles with 18 SNP sites were identified for MsPGIP2 in the alfalfa variety 'Algonquin'. In conclusion, MsPGIP2 possessed great genetic variation, and the addition of primers to the PCR products in combination with the fluorescent labeling of primers could significantly improve the sensitivity and resolution of SSCP analysis. This technique could be used for genetic diversity detection and marker-assisted breeding of useful genes in autopolyploid species such as alfalfa.

  12. The Varieties of Ignorance

    DEFF Research Database (Denmark)

    Nottelmann, Nikolaj

    2016-01-01

    This chapter discusses varieties of ignorance divided according to kind (what the subject is ignorant of), degree, and order (e.g. ignorance of ignorance equals second-order ignorance). It provides analyses of notions such as factual ignorance, erotetic ignorance (ignorance of answers to question...

  13. Logical Varieties in Normative Reasoning

    CERN Document Server

    Burgin, Mark; Mestdagh, de Vey

    2011-01-01

    Although conventional logical systems based on logical calculi have been successfully used in mathematics and beyond, they have definite limitations that restrict their application in many cases. For instance, the principal condition for any logical calculus is its consistency. At the same time, knowledge about large object domains (in science or in practice) is essentially inconsistent. Logical prevarieties and varieties were introduced to eliminate these limitations in a logically correct way. In this paper, the Logic of Reasonable Inferences is described. This logic has been applied successfully to model legal reasoning with inconsistent knowledge. It is demonstrated that this logic is a logical variety and properties of logical varieties related to legal reasoning are developed.

  14. Definite Article Usage across Varieties of English

    Science.gov (United States)

    Wahid, Ridwan

    2013-01-01

    This paper seeks to explore the extent of definite article usage variation in several varieties of English based on a classification of its usage types. An annotation scheme based on Hawkins and Prince was developed for this purpose. Using matching corpus data representing Inner Circle varieties and Outer Circle varieties, analysis was made on…

  15. Evaluation of ensilage potential of alfalfa cultivars (Medicago sativa L.)

    OpenAIRE

    Monteiro, ALG; Costa,Ciniro; Arrigoni, M. D.

    1998-01-01

    The objective of this trial was to study the ensilage potential of alfalfa (Medicago sativa L,.). The material used in the evaluation was harvested from an experiment in a complete randomized blocks design with three replicates, with seventeen alfalfa cultivars, Criouil, Florida 77, P555, P581, Moapa, Gilboa, 34 Linea, C/W 8754, C/W 4468, C/W 86, C/W 8746, Hunter Field, Trifecta, Sequel, CUF 101, Aurora e Siriver, during three years. The harvest used for the determinations was collected at 30...

  16. Differential Pathogenicity of Four Pratylenchus neglectus Populations on Alfalfa

    OpenAIRE

    1991-01-01

    A Pratylenchus neglectus population from lltah (UT3) was more virulent to Lahontan alfalfa than other P. neglectus populations from Utah (UT1, UT2) and Wyoming (WY). All alfalfa plants survived at 24 ± 3 C when inoculated with WY, UT1, or UT2 at initial populations (Pi) of 500, 1,000, and 5,000 nematodes per plant. At Pi 10,000 with WY, UT1, or UT2, plant mortality was 15, 15, and 20%, respectively; at Pi 5,000 and 10,000 with UT3, plant mortality was 10 and 40%. The WY, UT1, and UT2 populati...

  17. Investigation of Endosperm Cell Development of Different Rice Varieties%不同类型水稻品种胚乳发育的研究

    Institute of Scientific and Technical Information of China (English)

    李栋梁; 李小刚; 顾蕴洁; 王忠

    2014-01-01

    不同部位的分布上,4种水稻品种各自呈现了一定程度的差异与联系。粳稻2个品种与籼稻2个品种间差异更多体现在颖果发育进程上;而糯稻与非糯稻的区别则更多体现在淀粉体的形态与灌浆的充实程度上。%The objective of this research is to clarify the difference of rice endosperm of different varieties during the development process.[Method]Four rice varieties, Nipponbare, Yangdao 6, Wuyunuo16 and Yangfunuo 4 were used as the experimental materials, and their caryopsis development days precisely were precisely recorded and their caryopsis development was closely observed. Starch accumulation and physiological activities were observed by I2-KI and TTC staining. Structure changes of rice starch endosperm cells were observed by applying spur resin embedding and semi-thin sectioning, and light microscopy. The ultrastructure and element analysis of full ripe rice caryopsis were observed under scanning electron microscope and EDS. [Result]Four stages occurred in caryopses development of all the rice varieties tested: formation stage, milky stage, dough stage, and full maturity stage. Compared to development stages of the endosperm, formation stage runs in parallel to the coenocyte and cellurization stages, which are the initial stage of endosperm formation; milky stage corresponds to the differentiation stage of endosperm cells, while the last two stages, dough and full maturity stages, are equivalent to that of the endosperm maturity stage. The shape of two rice sub subspecies, japonica and indica, varies significantly. Of the four rice varieties tested, two indica varieties showed a relatively faster growth rate than two japonica cultivars; Wuyunuo16 and Yangfunuo 4 had almost identical growth rate of dry matter accumulation. Nuclei of starch endosperm cells degenerated with the extension of amyloplasts, while amyloplasts were still enlarging and growing. Amyloplasts in the endosperm cells were compound granules, and their

  18. 紫花苜蓿耐盐种质资源的遗传多样性分析%Analysis of genetic diversity of salt tolerant alfalfa germplasms

    Institute of Scientific and Technical Information of China (English)

    姜健; 杨宝灵; 夏彤; 于淑梅; 乌云娜

    2011-01-01

    利用RAPD技术分析了25个紫花苜蓿耐盐品种的遗传结构和遗传多样性.结果表明,30条引物在25个紫花苜蓿耐盐品种单株DNA间的多态性位点比率(P)为81.52%,在各品种混合DNA间的多态性位点比率为61.65%,说明采用单株DNA样品比采用混合DNA样品能更好地揭示紫花苜蓿品种内和品种间的遗传变异水平.基因分化系数(Gst)主要反应品种间变异占总变异的比例,中国18个耐盐紫花苜蓿品种和美国7个耐盐紫花苜蓿品种的基因分化系数分别为0.271和0.152,表明中国耐盐紫花苜蓿种质资源品种间基因交流机会比美国品种间交流机会多.紫花苜蓿作为典型的异交植物,其生物群体的遗传结构与其繁育体系具有直接的联系.依据遗传距离(GD)分析结果,25份材料从遗传结构上可以分为9个组群,其中图牧1号和图牧2号遗传距离最小(GD=0.148),捷达和图牧1号遗传距离最大(GD=0.786).紫花苜蓿耐盐种质遗传多样性分析为紫花苜蓿耐盐核心种质库构建和耐盐新品种选育提供了理论依据.%The genetic structure and diversity of 25 alfalfa (Medicago sativa) salt-tolerant varieties was ana lyzed by RAPD technology. Results showed that the polymorphism loci percentages (P) of 30 primers between single-plant DNA of 25 salt-tolerant alfalfa varieties were 81. 52%, and between mixed DNA of various varie ties were 61. 65%, indicating that single-plant DNA sample was a better method than mixed DNA sample, to reveal the level of genetic variation within and between alfalfa varieties. The coefficient of gene differentiation (Gst) mainly reflects the ratio of species variation to total variation, and the Gst of 18 Chinese salt-tolerant al falfa varieties and 7 American salt-tolerant alfalfa varieties were 0. 271 and 0. 152, respectively, showing that there was more interactive gene exchange between Chinese salt-tolerant alfalfa germplasm was than that of A merican germplasm

  19. Effects of alfalfa meal on carcase quality and fat metabolism of Muscovy ducks.

    Science.gov (United States)

    Jiang, J F; Song, X M; Huang, X; Wu, J L; Zhou, W D; Zheng, H C; Jiang, Y Q

    2012-01-01

    1. The effects of alfalfa meal on carcase quality and fat metabolism of Muscovy duck were evaluated. The objective of this research was to establish whether alfalfa meal can reduce fat content and improve carcase quality of Muscovy duck. Animal products with a high fat content present a risk factor for many diseases. Reducing fat content in poultry products is an important goal for the poultry industry. 2. A total of 240 14-d-old white Muscovy ducks were selected and randomly allocated to 1 of 4 dietary treatments containing 0, 3, 6, and 9% of alfalfa meal for 5 weeks. Growth performances were recorded and carcase characteristics and lipid parameters were analysed. 3. Results showed that 3, 6, and 9% alfalfa meal in diet had no significant effects on growth performance of Muscovy ducks from 14 to 49 d of age. Ducks given 3, 6, and 9% alfalfa meal had significantly higher dressing percentage and lower abdominal fat percentage compared with those given no alfalfa meal. Ducks given 9% alfalfa meal had higher breast meat percentage compared with those given no alfalfa meal. The concentrations of triglyceride, total cholesterol, low density lipoprotein (LDL), very low density lipoprotein (VLDL) and free fatty acid in serum of ducks fed on alfalfa meal decreased. Alfalfa meal in the diet decreased abdominal fat percentage and improved carcase traits of Muscovy duck. 4. The study showed that dietary alfalfa meal decreased abdominal fat percentage and improved carcase traits, without an adverse effect on performance.

  20. Occurrence of Transgenic Feral Alfalfa (Medicago sativa subsp. sativa L.) in Alfalfa Seed Production Areas in the United States.

    Science.gov (United States)

    Greene, Stephanie L; Kesoju, Sandya R; Martin, Ruth C; Kramer, Matthew

    2015-01-01

    The potential environmental risks of transgene exposure are not clear for alfalfa (Medicago sativa subsp. sativa), a perennial crop that is cross-pollinated by insects. We gathered data on feral alfalfa in major alfalfa seed-production areas in the western United States to (1) evaluate evidence that feral transgenic plants spread transgenes and (2) determine environmental and agricultural production factors influencing the location of feral alfalfa, especially transgenic plants. Road verges in Fresno, California; Canyon, Idaho; and Walla Walla, Washington were surveyed in 2011 and 2012 for feral plants, and samples were tested for the CP4 EPSPS protein that conveys resistance to glyphosate. Of 4580 sites surveyed, feral plants were observed at 404 sites. Twenty-seven percent of these sites had transgenic plants. The frequency of sites having transgenic feral plants varied among our study areas. Transgenic plants were found in 32.7%, 21.4.7% and 8.3% of feral plant sites in Fresno, Canyon and Walla Walla, respectively. Spatial analysis suggested that feral populations started independently and tended to cluster in seed and hay production areas, places where seed tended to drop. Significant but low spatial auto correlation suggested that in some instances, plants colonized nearby locations. Neighboring feral plants were frequently within pollinator foraging range; however, further research is needed to confirm transgene flow. Locations of feral plant clusters were not well predicted by environmental and production variables. However, the likelihood of seed spillage during production and transport had predictive value in explaining the occurrence of transgenic feral populations. Our study confirms that genetically engineered alfalfa has dispersed into the environment, and suggests that minimizing seed spillage and eradicating feral alfalfa along road sides would be effective strategies to minimize transgene dispersal.

  1. Occurrence of Transgenic Feral Alfalfa (Medicago sativa subsp. sativa L. in Alfalfa Seed Production Areas in the United States.

    Directory of Open Access Journals (Sweden)

    Stephanie L Greene

    Full Text Available The potential environmental risks of transgene exposure are not clear for alfalfa (Medicago sativa subsp. sativa, a perennial crop that is cross-pollinated by insects. We gathered data on feral alfalfa in major alfalfa seed-production areas in the western United States to (1 evaluate evidence that feral transgenic plants spread transgenes and (2 determine environmental and agricultural production factors influencing the location of feral alfalfa, especially transgenic plants. Road verges in Fresno, California; Canyon, Idaho; and Walla Walla, Washington were surveyed in 2011 and 2012 for feral plants, and samples were tested for the CP4 EPSPS protein that conveys resistance to glyphosate. Of 4580 sites surveyed, feral plants were observed at 404 sites. Twenty-seven percent of these sites had transgenic plants. The frequency of sites having transgenic feral plants varied among our study areas. Transgenic plants were found in 32.7%, 21.4.7% and 8.3% of feral plant sites in Fresno, Canyon and Walla Walla, respectively. Spatial analysis suggested that feral populations started independently and tended to cluster in seed and hay production areas, places where seed tended to drop. Significant but low spatial auto correlation suggested that in some instances, plants colonized nearby locations. Neighboring feral plants were frequently within pollinator foraging range; however, further research is needed to confirm transgene flow. Locations of feral plant clusters were not well predicted by environmental and production variables. However, the likelihood of seed spillage during production and transport had predictive value in explaining the occurrence of transgenic feral populations. Our study confirms that genetically engineered alfalfa has dispersed into the environment, and suggests that minimizing seed spillage and eradicating feral alfalfa along road sides would be effective strategies to minimize transgene dispersal.

  2. Varieties of lattices

    CERN Document Server

    Jipsen, Peter

    1992-01-01

    The study of lattice varieties is a field that has experienced rapid growth in the last 30 years, but many of the interesting and deep results discovered in that period have so far only appeared in research papers. The aim of this monograph is to present the main results about modular and nonmodular varieties, equational bases and the amalgamation property in a uniform way. The first chapter covers preliminaries that make the material accessible to anyone who has had an introductory course in universal algebra. Each subsequent chapter begins with a short historical introduction which sites the original references and then presents the results with complete proofs (in nearly all cases). Numerous diagrams illustrate the beauty of lattice theory and aid in the visualization of many proofs. An extensive index and bibliography also make the monograph a useful reference work.

  3. Varieties of update

    Directory of Open Access Journals (Sweden)

    Sarah E Murray

    2014-03-01

    Full Text Available This paper discusses three potential varieties of update: updates to the common ground, structuring updates, and updates that introduce discourse referents. These different types of update are used to model different aspects of natural language phenomena. Not-at-issue information directly updates the common ground. The illocutionary mood of a sentence structures the context. Other updates introduce discourse referents of various types, including propositional discourse referents for at-issue information. Distinguishing these types of update allows a unified treatment of a broad range of phenomena, including the grammatical evidentials found in Cheyenne (Algonquian as well as English evidential parentheticals, appositives, and mood marking. An update semantics that can formalize all of these varieties of update is given, integrating the different kinds of semantic contributions into a single representation of meaning. http://dx.doi.org/10.3765/sp.7.2 BibTeX info

  4. From model to crop: functional analysis of a STAY-GREEN gene in the model legume Medicago truncatula and effective use of the gene for alfalfa improvement.

    Science.gov (United States)

    Zhou, Chuanen; Han, Lu; Pislariu, Catalina; Nakashima, Jin; Fu, Chunxiang; Jiang, Qingzhen; Quan, Li; Blancaflor, Elison B; Tang, Yuhong; Bouton, Joseph H; Udvardi, Michael; Xia, Guangmin; Wang, Zeng-Yu

    2011-11-01

    Medicago truncatula has been developed into a model legume. Its close relative alfalfa (Medicago sativa) is the most widely grown forage legume crop in the United States. By screening a large population of M. truncatula mutants tagged with the transposable element of tobacco (Nicotiana tabacum) cell type1 (Tnt1), we identified a mutant line (NF2089) that maintained green leaves and showed green anthers, central carpels, mature pods, and seeds during senescence. Genetic and molecular analyses revealed that the mutation was caused by Tnt1 insertion in a STAY-GREEN (MtSGR) gene. Transcript profiling analysis of the mutant showed that loss of the MtSGR function affected the expression of a large number of genes involved in different biological processes. Further analyses revealed that SGR is implicated in nodule development and senescence. MtSGR expression was detected across all nodule developmental zones and was higher in the senescence zone. The number of young nodules on the mutant roots was higher than in the wild type. Expression levels of several nodule senescence markers were reduced in the sgr mutant. Based on the MtSGR sequence, an alfalfa SGR gene (MsSGR) was cloned, and transgenic alfalfa lines were produced by RNA interference. Silencing of MsSGR led to the production of stay-green transgenic alfalfa. This beneficial trait offers the opportunity to produce premium alfalfa hay with a more greenish appearance. In addition, most of the transgenic alfalfa lines retained more than 50% of chlorophylls during senescence and had increased crude protein content. This study illustrates the effective use of knowledge gained from a model system for the genetic improvement of an important commercial crop.

  5. The Undergraduate ALFALFA Team: A Model for Involving Undergraduates in Major Legacy Astronomy Research

    Science.gov (United States)

    Troischt, Parker; Koopmann, Rebecca A.; Haynes, Martha P.; Higdon, Sarah; Balonek, Thomas J.; Cannon, John M.; Coble, Kimberly A.; Craig, David; Durbala, Adriana; Finn, Rose; Hoffman, G. Lyle; Kornreich, David A.; Lebron, Mayra E.; Crone-Odekon, Mary; O'Donoghue, Aileen A.; Olowin, Ronald Paul; Pantoja, Carmen; Rosenberg, Jessica L.; Venkatesan, Aparna; Wilcots, Eric M.; Alfalfa Team

    2015-01-01

    The NSF-sponsored Undergraduate ALFALFA (Arecibo Legacy Fast ALFA) Team (UAT) is a consortium of 19 institutions founded to promote undergraduate research and faculty development within the extragalactic ALFALFA HI blind survey project and follow-up programs. The collaborative nature of the UAT allows faculty and students from a wide ​range of public and private colleges and especially those with small astronomy programs to develop scholarly collaborations. Components of the program include an annual undergraduate workshop at Arecibo Observatory, observing runs at Arecibo, computer infrastructure, summer and academic year research projects, and dissemination at national meetings (e.g., Alfvin et al., Martens et al., Sanders et al., this meeting). Through this model, faculty and students are learning how science is accomplished in a large collaboration while contributing to the scientific goals of a major legacy survey. In the 7 years of the program, 23 faculty and more than 220 undergraduate students have participated at a significant level. 40% of them have been women and members of underrepresented groups. Faculty, many of whom were new to the collaboration and had expertise in other fields, contribute their diverse sets of skills to ALFALFA ​related projects via observing, data reduction, collaborative research, and research with students. 142 undergraduate students have attended the annual workshops at Arecibo Observatory, interacting with faculty, graduate students, their peers, and Arecibo staff in lectures, group activities, tours, and observing runs. Team faculty have supervised 131 summer research projects and 94 academic year (e.g., senior thesis) projects. 62 students have traveled to Arecibo Observatory for observing runs and 46 have presented their results at national meetings. 93% of alumni are attending graduate school and/or pursuing a career in STEM. Half of those pursuing graduate degrees in Physics or Astronomy are women. This work has been

  6. Diversity of field isolates of sinorhizobium meliloti nodulating alfalfa

    Science.gov (United States)

    Most alfalfa seed is treated with a rhizobial inoculant consisting of one or more strains of Sinorhizobium meliloti before planting to enhance nodulation of seedlings. However, little is known about the persistence of inoculated strains later in the season. There is also a paucity of information on ...

  7. Oxidative burst in alfalfa-Sinorhizobium meliloti symbiotic interaction.

    Science.gov (United States)

    Santos, R; Hérouart, D; Sigaud, S; Touati, D; Puppo, A

    2001-01-01

    Reactive oxygen species are produced as an early event in plant defense response against avirulent pathogens. We show here that alfalfa responds to infection with Sinorhizobium meliloti by production of superoxide and hydrogen peroxide. This similarity in the early response to infection by pathogenic and symbiotic bacteria addresses the question of which mechanism rhizobia use to counteract the plant defense response.

  8. Impacts on potential ethanol and crude protein yield in alfalfa

    Science.gov (United States)

    An alfalfa (Medicago sativa L.) biomass energy production system would produce two products. Leaves would be separated from stems to produce a high protein feed for livestock while stems would be processed to produce ethanol. Therefore, maximum yields of both leaves and stems are essential for profi...

  9. Modeling of desorption of Alfalfa (Medicago sativa) stems and leaves.

    NARCIS (Netherlands)

    ArabHosseini, A.; Huisman, W.; Müller, J.

    2011-01-01

    The equilibrium moisture content of agricultural products is necessary to optimize drying process and helps to keep the quality of the product during the period of storage. The main aim of this research was to find the best model which could define well, the exchange of moisture between alfalfa (Med

  10. PHENOTYPIC DIVERSITY OF ALFALFA (MEDICAGO SATIVA L. GERMPLASM

    Directory of Open Access Journals (Sweden)

    Marijana Tucak

    2011-06-01

    Full Text Available The objective of this study was to evaluate phenotypic diversity in the alfalfa germplasm collections using multivariate analysis to examine the extent of genetic diversity and contribution of selected characters to the total diversity and finally to select the most promising clusters/populations for further breeding work. Forty alfalfa populations/cultivars of different geographical origin were evaluated for 12 agro-morphological characters during two consecutive years. The populations/ cultivars were grouped into six clusters. In most cases populations/cultivars within clusters were not associated with their geographical origin. Intercluster distances were larger than the intracluster ones. This research revealed a broad phenotypic diversity within and between the alfalfa germplasm collections. The following characters contributed most to the total phenotypic diversity: dry matter yield in the first production year, plant height and length of central leaflet. Based on the mean value of the evaluated characters and determined distances between clusters, the most promising populations/cultivars belong to the clusters IV and V. Selected populations/cultivars could be considered as a valuable genetic material for the yield and quality improvement of alfalfa in our breeding programme.

  11. Evaluation of alfalfa-tall fescue mixtures across multiple environments

    Science.gov (United States)

    Binary grass-legume mixtures can benefit forage production systems in different ways helping growers cope both with increasing input costs (e.g., N fertilizer, herbicides) and potentially more variable weather. The main objective of this study was to evaluate alfalfa (Medicago sativa L.) and tall f...

  12. Lignin modification leads to increased nodule numbers in alfalfa.

    Science.gov (United States)

    Gallego-Giraldo, Lina; Bhattarai, Kishor; Pislariu, Catalina I; Nakashima, Jin; Jikumaru, Yusuke; Kamiya, Yuji; Udvardi, Michael K; Monteros, Maria J; Dixon, Richard A

    2014-03-01

    Reduction of lignin levels in the forage legume alfalfa (Medicago sativa) by down-regulation of the monolignol biosynthetic enzyme hydroxycinnamoyl coenzyme A:shikimate hydroxycinnamoyl transferase (HCT) results in strongly increased digestibility and processing ability of lignocellulose. However, these modifications are often also associated with dwarfing and other changes in plant growth. Given the importance of nitrogen fixation for legume growth, we evaluated the impact of constitutively targeted lignin modification on the belowground organs (roots and nodules) of alfalfa plants. HCT down-regulated alfalfa plants exhibit a striking reduction in root growth accompanied by an unexpected increase in nodule numbers when grown in the greenhouse or in the field. This phenotype is associated with increased levels of gibberellins and certain flavonoid compounds in roots. Although HCT down-regulation reduced biomass yields in both the greenhouse and field experiments, the impact on the allocation of nitrogen to shoots or roots was minimal. It is unlikely, therefore, that the altered growth phenotype of reduced-lignin alfalfa is a direct result of changes in nodulation or nitrogen fixation efficiency. Furthermore, HCT down-regulation has no measurable effect on carbon allocation to roots in either greenhouse or 3-year field trials.

  13. Paternity testing in an autotetraploid alfalfa breeding polycross

    Science.gov (United States)

    Determining unknown parentage in autotetraploid alfalfa (Medicago sativa L.) (2n = 4x = 32) can improve breeding gains. Exclusion analysis based paternity testing SAS code is presented, amenable to genotyping errors, for autotetraploid species utilizing co-dominant molecular markers with ambiguous d...

  14. Managing puncturevine in alfalfa hay and along field edges

    Science.gov (United States)

    Puncturevine (Tribulus terrestris) is a nuisance and difficult to control weed in alfalfa hay field edges and borders. Puncturevine contaminated hay can contain high levels of nitrates and burs can injure mouths of livestock, lowering the value and quality of the hay. Puncturevine is a summer annual...

  15. PERACETIC ACID PRETREATMENT OF ALFALFA STEM AND ASPEN BIOMASS

    Directory of Open Access Journals (Sweden)

    Lei Xu,

    2011-11-01

    Full Text Available Alfalfa stems and ground aspen were exposed to peracetic acid (0.5 to 9% on biomass at temperatures ranging from 40 to 100° C and reaction times from 1 to 5 hours. Glucose release as a percentage of total cellulose content was determined using subsequent standard enzymatic hydrolysis. Statistical analysis confirmed that aspen showed a strong response to peracetic acid addition rate. 9% peracetic acid removed 14% of the original lignin and increased the rate of glucose release from 23% to 44%. Temperature and reaction time played a less significant role. For alfalfa stems, low levels of peractic acid (0.5% increased glucose release from 30 to 47%. The addition of larger doses of peracetic acid did not show any significant improvement; this effect appears to be closely related to rate of lignin removal. While peracetic acid effectively removed lignin from aspen, 98% of the original lignin was still present in alfalfa after higher level peracetic acid treatments; the yield loss observed during pretreatment of alfalfa stems originates from other biomass components.

  16. Characterization of alfalfa populations contrasting for root system architecture (RSA)

    Science.gov (United States)

    The root system architecture affects the capacity for nutrient and water uptake thus impacting biomass yield production and may contribute to the persistence of perennial plants. The objectives of this study were to phenotype the roots of three alfalfa populations and identify differences between di...

  17. Using RNA-Seq for gene identification, polymorphism detection and transcript profiling in two alfalfa genotypes with divergent cell wall composition in stems

    Directory of Open Access Journals (Sweden)

    Lamb JoAnn FS

    2011-04-01

    Full Text Available Abstract Background Alfalfa, [Medicago sativa (L. sativa], a widely-grown perennial forage has potential for development as a cellulosic ethanol feedstock. However, the genomics of alfalfa, a non-model species, is still in its infancy. The recent advent of RNA-Seq, a massively parallel sequencing method for transcriptome analysis, provides an opportunity to expand the identification of alfalfa genes and polymorphisms, and conduct in-depth transcript profiling. Results Cell walls in stems of alfalfa genotype 708 have higher cellulose and lower lignin concentrations compared to cell walls in stems of genotype 773. Using the Illumina GA-II platform, a total of 198,861,304 expression sequence tags (ESTs, 76 bp in length were generated from cDNA libraries derived from elongating stem (ES and post-elongation stem (PES internodes of 708 and 773. In addition, 341,984 ESTs were generated from ES and PES internodes of genotype 773 using the GS FLX Titanium platform. The first alfalfa (Medicago sativa gene index (MSGI 1.0 was assembled using the Sanger ESTs available from GenBank, the GS FLX Titanium EST sequences, and the de novo assembled Illumina sequences. MSGI 1.0 contains 124,025 unique sequences including 22,729 tentative consensus sequences (TCs, 22,315 singletons and 78,981 pseudo-singletons. We identified a total of 1,294 simple sequence repeats (SSR among the sequences in MSGI 1.0. In addition, a total of 10,826 single nucleotide polymorphisms (SNPs were predicted between the two genotypes. Out of 55 SNPs randomly selected for experimental validation, 47 (85% were polymorphic between the two genotypes. We also identified numerous allelic variations within each genotype. Digital gene expression analysis identified numerous candidate genes that may play a role in stem development as well as candidate genes that may contribute to the differences in cell wall composition in stems of the two genotypes. Conclusions Our results demonstrate that RNA

  18. Transcriptome Analyses Reveal Candidate Genes Potentially Involved in Al Stress Response in Alfalfa

    Science.gov (United States)

    Liu, Wenxian; Xiong, Conghui; Yan, Longfeng; Zhang, Zhengshe; Ma, Lichao; Wang, Yanrong; Liu, Yajie; Liu, Zhipeng

    2017-01-01

    Alfalfa is the most extensively cultivated forage legume, yet most alfalfa cultivars are not aluminum tolerant, and the molecular mechanisms underlying alfalfa responses to Al stress are largely unknown. In this study, we aimed to understand how alfalfa responds to Al stress by identifying and analyzing Al-stress-responsive genes in alfalfa roots at the whole-genome scale. The transcriptome changes in alfalfa roots under Al stress for 4, 8, or 24 h were analyzed using Illumina high-throughput sequencing platforms. A total of 2464 differentially expressed genes (DEGs) were identified, and most were up-regulated at early (4 h) and/or late (24 h) Al exposure time points rather than at the middle exposure time point (8 h). Metabolic pathway enrichment analysis demonstrated that the DEGs involved in ribosome, protein biosynthesis, and process, the citrate cycle, membrane transport, and hormonal regulation were preferentially enriched and regulated. Biosynthesis inhibition and signal transduction downstream of auxin- and ethylene-mediated signals occur during alfalfa responses to root growth inhibition. The internal Al detoxification mechanisms play important roles in alfalfa roots under Al stress. These findings provide valuable information for identifying and characterizing important components in the Al signaling network in alfalfa and enhance understanding of the molecular mechanisms underlying alfalfa responses to Al stress. PMID:28217130

  19. In vivo characterization of the effects of abscisic acid and drying protocols associated with the acquisition of desiccation tolerance in alfalfa (Medicago sativa L.) somatic embryos

    NARCIS (Netherlands)

    Sreedhar, L.; Wolkers, W.F.; Hoekstra, F.A.; Bewley, J.D.

    2002-01-01

    Although somatic embryos of alfalfa (Medicago sativa L.) had acquired some tolerance to desiccation at the cotyledonary stage of development (22 d after plating), additional culturing in 20 ?M abscisic acid (ABA) for 8 d induced greater desiccation tolerance, as determined by increased germination.

  20. Growth With of Alfalfa Mutant in Different Nitrogen Fertilizer and Defoliation Intensity

    Directory of Open Access Journals (Sweden)

    Widyati Slamet

    2012-10-01

    Full Text Available The research was conducted to evaluate growth  of alfalfa mutan (plant height increment, number of leaves and dry matter production in different  Nitrogen Fertilizer and defoliation intensity. The design used was randomized block design 4x2 factorial with 3 replications. The first was dosage of Nitrogen fertilizerNitrogen   (0, 30, 60 and 90 kg N / ha,  the second factor was defoliation intensity (5 and 10 cm. Variables observed alfalfa growth (plant height increment, number of leaves, the production of dry matter (DM forage. The results showed that different N fertilization did not affect the growth of alfalfa mutants. Defoliation intensity  affectedmnumber of leaves and   DM  production of alfalfa mutant. Fertilization to 90 kg N / ha has not affected the growth and   defoliation  intensity 10 cm gave better growth on alfalfa mutant.   [Keywords: alfalfa mutant; Nitrogen fertilizer; defoliation; growth

  1. Performance of Three Adelphocoris spp. (Hemiptera:Miridae) on Flowering and Non-lfowering Cotton and Alfalfa

    Institute of Scientific and Technical Information of China (English)

    GAO Zhen; PAN Hong-sheng; LIU Bing; LU Yan-hui; LIANG Ge-mei

    2014-01-01

    The genus Adelphocoris (Hemiptera:Miridae) is a group of key insect pests in cotton ifelds in China that includes three dominant species:A. suturalis (Jakovlev), A. lineolatus (Goeze) and A. fasciaticollis (Reuter). Previous ifeld surveys have found that adults of these Adelphocoris species usually move onto speciifc host plants when the plants enter the lfowering stage. To determine the potential trade-offs for this host-plant preference behavior, the performance of these three Adelphocoris spp. on lfowering and non-lfowering cotton and alfalfa were compared in the laboratory. The results showed that Adelphocoris spp. had signiifcantly higher nymphal developmental and survival rates, along with increased adult longevity and fecundity on lfowering cotton and alfalfa than on non-lfowering plants of either species. In addition, compared with cotton plants, alfalfa generally promoted better performance of these three Adelphocoris species, especially for A. lineolatus. Simple correlation analysis indicated that female adult longevity was positively correlated to male adult longevity and female fecundity, and female fecundity was positively correlated to nymphal development and survival rates. This study demonstrated a positive correlation between adult preference and offspring/adult performance for all three Adelphocoris species, with no evidence of any trade-offs for this preference for lfowering host plants. These ifndings support the hypothesis that hemimetabolous insects such as mirid bugs have positive adult preference-adult/nymphal performance relationships, which is likely due to the similar feeding habits and nutritional requirements of adults and nymphs.

  2. Effect of nitrogen fertilization on yield, N content, and nitrogen ifxation of alfalfa and smooth bromegrass grown alone or in mixture in greenhouse pots

    Institute of Scientific and Technical Information of China (English)

    XIE Kai-yun; LI Xiang-lin; HE Feng; ZHANG Ying-jun; WAN Li-qiang; David B Hannaway; WANG Dong; QIN Yan; Gamal M A Fadul

    2015-01-01

    grown alone (shoots:|t|=3.39, P=0.0096;root:|t|=3.57, P=0.0073). We believe this was due to smooth bromegrass being better able to absorb available soil N (due to its ifbrous root system), resulting in lower soil N availability and al owing alfalfa to develop an effective N2 ifxing symbiosis prior to the 1st harvest. Once soil N levels were depleted, alfalfa was able to ifx N2, resulting in the majority of its tissue N being derived from biological nitrogen ifxation (BNF) in the 2nd and 3rd harvests. When grown in a mixture, with added N, alfalfa established an effective symbiosis earlier than when grown alone;in monoculture BNF did not contribute a signiifcant portion of plant N in the N75 and N150 treatments, whereas in the mixture, BNF contributed 17.90 and 16.28%for these treatments respectively. Alfalfa has a higher BNF efifciency when grown in a mixture, initiating BNF earlier, and having higher N2 ifxation due to less inhibition by soil-available N. For the greatest N-use-efifciency and sustainable production, grass-legume mixtures are recommended for improving grasslands, using a moderate amount of N fertilizer (75 kg N ha–1) to provide optimum beneifts.

  3. Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis

    OpenAIRE

    Monteiro, Mariza; Appezzato-da-Glória, Beatriz; Valarini,Maria José; Oliveira, Carlos Alberto; Vieira, Maria Lucia Carneiro

    2003-01-01

    Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this pro...

  4. Transcriptome Analysis of Resistant and Susceptible Alfalfa Cultivars Infected With Root-Knot Nematode Meloidogyne incognita

    OpenAIRE

    Postnikova, Olga A.; Maria Hult; Jonathan Shao; Andrea Skantar; Nemchinov, Lev G.

    2015-01-01

    Nematodes are one of the major limiting factors in alfalfa production. Root-knot nematodes (RKN, Meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. As of today, no studies have been published on global gene expression profiling in alfalfa infected with RKN or any other plant parasitic nematode. Very little information is available about molecular mechanisms that contribute...

  5. Animal feed compositions containing phytase derived from transgenic alfalfa and methods of use thereof

    Science.gov (United States)

    Austin-Phillips, Sandra; Koegel, Richard G.; Straub, Richard J.; Cook, Mark

    1999-01-01

    A value-added composition of matter containing plant matter from transgenic alfalfa which expresses exogenous phytase activity is disclosed. The phytase activity is a gene product of an exogenous gene encoding for phytase which has been stably incorporated into the genome of alfalfa plants. The transgenic alfalfa expresses phytase activity in nutritionally-significant amounts, thereby enabling its use in animal feeds to eliminate the need for phosphorous supplementation of livestock, poultry, and fish feed rations.

  6. Animal feed compositions containing phytase derived from transgenic alfalfa and methods of use thereof

    Energy Technology Data Exchange (ETDEWEB)

    Austin-Phillips, Sandra (Madison, WI); Koegel, Richard G. (Madison, WI); Straub, Richard J. (Brooklyn, WI); Cook, Mark (Madison, WI)

    2001-01-01

    A value-added composition of matter containing plant matter from transgenic alfalfa which expresses exogenous phytase activity is disclosed. The phytase activity is a gene product of an exogenous gene encoding for phytase which has been stably incorporated into the genome of alfalfa plants. The transgenic alfalfa expresses phytase activity in nutritionally-significant amounts, thereby enabling its use in animal feeds to eliminate the need for phosphorous supplementation of livestock, poultry, and fish feed rations.

  7. Toward characterizing seed vigor in alfalfa through proteomic analysis of germination and priming.

    Science.gov (United States)

    Yacoubi, Rafika; Job, Claudette; Belghazi, Maya; Chaibi, Wided; Job, Dominique

    2011-09-02

    Alfalfa, the most widely grown leguminous crop in the world, is generally exposed to severe salinity stress in Tunisia, notably affecting its germination performance. Toward a better understanding of alfalfa seed vigor, we have used proteomics to characterize protein changes occurring during germination and osmopriming, a pretreatment that accelerates germination and improves seedling uniformity particularly under stress conditions. The data revealed that germination was accompanied by dynamic changes of 79 proteins, which are mainly involved in protein metabolism, cell structure, metabolism, and defense. Comparative proteomic analysis also revealed 63 proteins specific to osmopriming, 65 proteins preferentially varying during germination, and 14 proteins common to both conditions. Thus, the present study unveiled the unexpected finding that osmopriming cannot simply be considered as an advance of germination-related processes but involves other mechanisms improving germination such as the mounting of defense mechanisms enabling osmoprimed seeds to surmount environmental stresses potentially occurring during germination. The present results therefore provide novel avenues toward understanding the mechanisms of invigoration of low vigor seeds by priming treatments that are widely used both in commercial applications and in developing countries (on farm seed priming) to better control crop yields.

  8. Seed Priming to Overcome Salinity Stress in Persian Cultivars of Alfalfa (Medicago sativa L.

    Directory of Open Access Journals (Sweden)

    Ali SEPEHRI

    2015-03-01

    Full Text Available In order to investigate the effect of hydro-priming on seed germination with distilled water on germination of five Alfalfa cultivars under salinity stress, an experiment was conducted as a factorial experiment based on a completely randomized design with three replications. Seven levels of hydro-priming and salinity of NaCl including prime and non-salinity, prime and 50 mM salinity, prime and 100 mM salinity, prime and 150 mM salinity, prime and 200 mM salinity, prime and 250 mM salinity and without prime and salinity and five alfalfa varieties, including ‘Hamedani’, ‘Isfahani’, ‘Bami’, ‘Yazdi’ and ‘Ghareh Yonjeh’ were used. The results showed that the main effect of prime, salinity and cultivars and their interaction in all studied traits were significantly affected at the 5% probability level. Priming treatments in non-salinity of all cultivars were the highest. In all cultivars, final germination percentage, length and weight of radicle, plumule and seedling, germination rate and time, relative radicle elongation, vigor index and stress index, were significantly improved in response to priming in salinity levels of 50-200 mM, compared to control. Radicle produced higher length and weight than the plumule in hydro- priming and salinity treatments. ‘Hamedani’ cultivar in most of studied characteristics had a better response than others. The lowest response to salinity stress and priming was observed in ‘Yazdi’ cultivar.

  9. Use of electrophoretic techniques in determining the composition of seed storage proteins in alfalfa.

    Science.gov (United States)

    Krochko, J E; Bewley, J D

    1988-11-01

    Holoprotein molecular weights and polypeptide composition can be determined for complex mixtures of oligomeric proteins using two-dimensional electrophoretic techniques. The variety of two-dimensional analyses presented here is a reflection of the general usefulness of each method for the identification and characterization of the different classes of seed storage proteins in alfalfa. These techniques can be applied to studies of storage proteins in other seeds as well as non-seed storage proteins. The major seed storage proteins in alfalfa are medicagin (a legumin-like globulin), alfin (a vicilin-like globulin) and a family of lower molecular weight albumins (LMW1-3). These comprise 30%, 10%, and 20%, respectively, of the total extractable protein from cotyledons of mature seeds. Alfin is a heterogeneous oligomeric protein (Mr approximately 150,000) composed of polypeptides ranging in size from Mr 14,000 to 50,000 (alpha 1-alpha 6; 50,000, 38,000, 32,000, 20,000, 16,000 and 14,000, respectively). Medicagin is also a high molecular weight oligomeric protein, but requires high concentrations of salt for solubilisation. It is comprised of a family of individually distinct subunits, each composed of an acidic polypeptide (A1-A9; Mr 49,000 to 39,000) linked via disulphide bond(s) to a basic polypeptide (B1, B2, B3; Mr 24,000, 23,000 and 20,000, respectively). This pairing is highly specific and two families are recognizable on the basis of the B polypeptide (B3 or B1/B2). Subunits (Mr approximately 50,000-65,000) are assembled as trimers (8S) or larger oligomers (12S-15S) in mature seeds. The lower molecular weight albumins (LMW1-3) are acidic (pI less than 6), and consist of sets of disulphide-bonded polypeptides (Mr 15,000 and 11,000).

  10. 我国加工番茄新品种应用现状与发展趋势%Application status and development trend of new processing tomato varieties in China

    Institute of Scientific and Technical Information of China (English)

    曾沂辉

    2015-01-01

    介绍加工番茄品种应用历程,新品种研究与市场开发现状,分析种子市场存在的问题和发展趋势,提出研究方向和建议。%This article introduces the application process of processing tomato varieties, the research of new varieties and market development. The research direction and suggestions were put forward after analyzing the problems and development trend of seed markets.

  11. Bioinformatics Analysis of Zinc Transporter from Baoding Alfalfa

    Institute of Scientific and Technical Information of China (English)

    Haibo WANG; Junyun GUO

    2012-01-01

    [Objective] This study aimed to perform the bioinformatics analysis of Zinc transporter (ZnT) from Baoding Alfalfa. [Method] Based on the amino acid sequence, the physical and chemical properties, hydrophilicity/hydrophobicity, secondary structure of ZnT from Baoding alfalfa were predicted by a series of bioinformatics software. And the transmembrane domains were predicted by using different online tools. [Result] ZnT is a hydrophobic protein containing 408 amino acids with the theoretical pl of 5.94, and it has 7 potential transmembrane hydrophobic regions. In the sec- ondary structure, co-helix (Hh) accounted for 48.04%, extended strand (Ee) for 9.56%, random coil (Cc) for 42.40%, which was accored with the characteristic of transmembrane protein. [Conclusion] mZnT is a member of CDF family, responsible for transporting Zn^2+ out of the cell membrane to reduce the concentration and toxicity of Zn^2+.

  12. Proteomics analysis of alfalfa response to heat stress.

    Directory of Open Access Journals (Sweden)

    Weimin Li

    Full Text Available The proteome responses to heat stress have not been well understood. In this study, alfalfa (Medicago sativa L. cv. Huaiyin seedlings were exposed to 25 °C (control and 40 °C (heat stress in growth chambers, and leaves were collected at 24, 48 and 72 h after treatment, respectively. The morphological, physiological and proteomic processes were negatively affected under heat stress. Proteins were extracted and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE, and differentially expressed protein spots were identified by mass spectrometry (MS. Totally, 81 differentially expressed proteins were identified successfully by MALDI-TOF/TOF. These proteins were categorized into nine classes: including metabolism, energy, protein synthesis, protein destination/storage, transporters, intracellular traffic, cell structure, signal transduction and disease/defence. Five proteins were further analyzed for mRNA levels. The results of the proteomics analyses provide a better understanding of the molecular basis of heat-stress responses in alfalfa.

  13. Co-expression of bacterial aspartate kinase and adenylylsulfate reductase genes substantially increases sulfur amino acid levels in transgenic alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Tong, Zongyong; Xie, Can; Ma, Lei; Liu, Liping; Jin, Yongsheng; Dong, Jiangli; Wang, Tao

    2014-01-01

    Alfalfa (Medicago sativa L.) is one of the most important forage crops used to feed livestock, such as cattle and sheep, and the sulfur amino acid (SAA) content of alfalfa is used as an index of its nutritional value. Aspartate kinase (AK) catalyzes the phosphorylation of aspartate to Asp-phosphate, the first step in the aspartate family biosynthesis pathway, and adenylylsulfate reductase (APR) catalyzes the conversion of activated sulfate to sulfite, providing reduced sulfur for the synthesis of cysteine, methionine, and other essential metabolites and secondary compounds. To reduce the feedback inhibition of other metabolites, we cloned bacterial AK and APR genes, modified AK, and introduced them into alfalfa. Compared to the wild-type alfalfa, the content of cysteine increased by 30% and that of methionine increased substantially by 60%. In addition, a substantial increase in the abundance of essential amino acids (EAAs), such as aspartate and lysine, was found. The results also indicated a close connection between amino acid metabolism and the tricarboxylic acid (TCA) cycle. The total amino acid content and the forage biomass tested showed no significant changes in the transgenic plants. This approach provides a new method for increasing SAAs and allows for the development of new genetically modified crops with enhanced nutritional value.

  14. New early indica variety Zhongyouzao 3

    Institute of Scientific and Technical Information of China (English)

    HUANGFasong; HUPeisong; TANGShaoqing; LUOJu

    1997-01-01

    Rice variety Zhongyouzao 3 was developed by CNRRI and Hunan Rice Research Institute,Hunan Province, from the cross 84-240/Hongtu 5 made in 1986. The 84-240 is the second season indica line with superior grain quality and multl-reslstance to rice pests, and the Hongtu 5 is early indica variety with highyielding.

  15. Identifying OH Imposters in the ALFALFA Neutral Hydrogen Survey

    CERN Document Server

    Suess, Katherine A; Haynes, Martha P; Giovanelli, Riccardo

    2016-01-01

    OH megamasers (OHMs) are rare, luminous molecular masers that are typically observed in (ultra) luminous infrared galaxies and serve as markers of major galaxy mergers. In blind emission line surveys such as the Arecibo Legacy Fast Arecibo L-Band Feed Array (ALFALFA) survey for neutral hydrogen (HI), OHMs at z~0.2 can mimic z~0.05 HI lines. We present the results of optical spectroscopy of ambiguous HI detections in the ALFALFA 40% data release detected by the Wide Field Infrared Survey Explorer (WISE) but with uncertain optical counterparts. The optical redshifts, obtained from observations at the Apache Point Observatory, revealed five new OHMs and identified 129 HI optical counterparts. Sixty candidates remain ambiguous. The new OHMs are the first detected in a blind spectral line survey. The number of OHMs in ALFALFA is consistent with predictions from the OH luminosity function. Additionally, the mid-infrared magnitudes and colors of the OHM host galaxies found in a blind survey do not seem to differ fro...

  16. Weed suppression ability of spring barley varieties

    DEFF Research Database (Denmark)

    Christensen, Svend

    1995-01-01

    Three years of experiments with spring barley showed significant differences in weed suppression ability among varieties. Weed dry matter in the most suppressive variety, Ida, was 48% lower than the mean weed dry matter of all varieties, whereas it was 31% higher in the least suppressive variety......, Grit. Ranking varietal responses to weed competition in terms of grain yield loss corresponded well to ranking weed dry matter produced in crop weed mixtures. There was no correspondence between the varietal grain yields in pure stands and their competitiveness, suggesting that breeding to optimize...... interception model was developed to describe the light interception profiles of the varieties. A study of the estimated parameters showed significant correlation between weed dry matter, rate of canopy height development and the light interception profile. However, when estimates were standardized to eliminate...

  17. Closed surfaces and character varieties

    CERN Document Server

    Chesebro, Eric

    2012-01-01

    The powerful character variety techniques of Culler and Shalen can be used to find essential surfaces in knot manifolds. We show that module structures on the coordinate ring of the character variety can be used to identify detected boundary slopes as well as when closed surfaces are detected. This approach also yields new number theoretic invariants for the character varieties of knot manifolds.

  18. Alfalfa interseeded into silage corn can serve as a cover crop and subsequent forage crop

    Science.gov (United States)

    Alfalfa (Medicago sativa) and corn (Zea mays) silage are commonly grown in rotation in dairy forage production systems throughout the northern regions of the USA. Alfalfa interseeded into silage corn could potentially serve two purposes: as a cover crop during the silage corn production year, and as...

  19. Mechanisms of qualitative and quantitative resistance to Aphanomyces root rot in alfalfa

    Science.gov (United States)

    Aphanomyces root rot (ARR), caused by Aphanomyces euteiches, is one of the most important diseases of alfalfa (Medicago sativa) in the United States. Two races of the pathogen are currently recognized. Most modern alfalfa cultivars have high levels of resistance to race 1 but few cultivars have resi...

  20. Mapping fall dormancy and winter injury in tetraploid alfalfa (Medicago sativa L.)

    Science.gov (United States)

    Alfalfa is a widely planted perennial forage crop. Dormancy in autumn (fall dormancy) is generally negatively correlated with winter injury in alfalfa. To understand the genetic basis of the two traits, we identified quantitative trait loci (QTL) controlling autumn growth and winter injury using a...

  1. Extraction, composition, and functional properties of dried alfalfa (Medicago sativa L.) leaf protein

    Science.gov (United States)

    Alfalfa, traditionally used for animal feed, has attracted attention as a potential feedstock for biofuels and the viability of the process would be enhanced by co-products with value-added uses. This study describes extraction of protein from dried alfalfa leaves and the functional properties of th...

  2. Prohexadione-calcium improves stand density and yield of alfalfa interseeded into silage corn

    Science.gov (United States)

    Interseeded alfalfa (Medicago sativa L.) could serve as a dual-purpose crop to provide groundcover for silage corn (Zea mays L.) and forage during subsequent years of production, but interspecific competition often leads to poor stands of alfalfa and unsatisfactory yields of corn. Four experiments e...

  3. Roadside alfalfa: Innocent bystanders or conveyers of genetically-engineered traits?

    Science.gov (United States)

    Clumps of alfalfa are a common sight along roads and vacant lots in areas that grow alfalfa for hay or seed. So what role do feral roadside plants play in dispersing transgenes? Is there a risk that transgenic feral plants serve as reservoirs or conduits that might facilitate the movement of transg...

  4. Interaction of bale size and preservative rate for large-round bales of alfalfa hay

    Science.gov (United States)

    Recently, two studies conducted at the US Dairy Forage Research Center have reported inconsistent storage responses following the application of propionic-acid-based preservatives to alfalfa or alfalfa-orchardgrass hays. One of these studies utilized 5-foot-diameter round bales, and produced disappo...

  5. A mineral seed coating for control of seedling diseases of alfalfa suitable for organic production systems

    Science.gov (United States)

    Most alfalfa seed is treated with the systemic fungicide mefenoxam (Apron XL) for control of soilborne seedling diseases. However, Apron XL does not have activity against Aphanomyces euteiches, the causal agent of Aphanomyces root rot (ARR), which is an important component of the alfalfa root rot co...

  6. Effects of dairy slurry on the nutritive value and fermentation characteristics of alfalfa silages

    Science.gov (United States)

    Dairy producers frequently ask questions about the risks associated with applying dairy slurry onto growing alfalfa. Our objectives were to determine the effects of dairy-slurry application on the subsequent nutritive value and fermentation characteristics of alfalfa silages. Dairy slurry was applie...

  7. Effects of alfalfa meal on the intestinal microbial diversity and immunity of growing ducks.

    Science.gov (United States)

    Jiang, J F; Song, X M; Wu, J L; Jiang, Y Q

    2014-12-01

    This study was conducted to investigate the effects of alfalfa meal diets on the intestinal microbial diversity and immunity of growing egg-type ducks. A total of 128 healthy 7-week-old female egg-type Shaoxing ducks were selected and randomly assigned into four dietary treatments: 0%, 3%, 6% and 9% alfalfa meal for 8 weeks. Each treatment consisted of four replicates of eight ducks each. Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was used to characterize the microbiota. The results showed that the DGGE fingerprints of the V6-V8 fragments of the 16S rRNA from the caeca and faeces of ducks fed 3%, 6% and 9% alfalfa meal had significantly higher microbiota species richness than those fed 0% alfalfa meal (p ducks fed 3%, 6% and 9% alfalfa meal was significantly higher than those fed 0% alfalfa meal (p 0.05), and the 3-9% alfalfa meal did not affect the growth performance of the growing egg-type ducks. The proliferation of T and B lymphocytes was significantly greater (p ducks. Dietary alfalfa meal supplementation increases intestinal microbial community diversity and improves of the immune response growing egg-type ducks.

  8. The Productivity and Quality of Alfalfa (Medicago sativa L. in Romanian Forest Steppe

    Directory of Open Access Journals (Sweden)

    Mihai STAVARACHE

    2015-04-01

    Full Text Available Alternative use of alfalfa, for various purposes, including the production of biofuels or food supplement for human alimentation, is a study topic still in its early stages of research. Studying and understanding the biology of alfalfa and the factors with a major influence on it are very important activities. The productivity and quality of alfalfa are two indicators that help determine, in addition to economic value, the way in which alfalfa can be used. Evolution of alfalfa yield and quality depends on many factors, such as the growth stage of alfalfa plants at harvesting. It was observed over three years of vegetation the influence of alfalfa plant growth stage at harvest on plant height, leaves/stems ratio, production of leaves, stems and whole plant (DM - dry matter per hectare and on quality indicators (CP - crude protein, NDF - neutral detergent fiber and ADF - acid detergent fiber. The results showed that, with the advancement of phenological phases, from early bud stage to complete flowering, the total biomass output raised from 2.79 Mg·ha-1 to 4.60 Mg·ha-1, the neutral detergent fiber raised from 48.4-50.6% to 62.0-67.7%, while crude protein content decreased from 21.2-24.0% to 13.3-16.5%. The parameter values were correlated with alfalfa growth stage during the harvesting (significant at the 0.05 and 0.01 probability levels.

  9. Economics of growth regulator treatment of alfalfa seed for interseeding into silage corn

    Science.gov (United States)

    Previous studies have focused on interseeding of alfalfa into corn for use as a temporary cover crop rather than as a means of jump-starting alfalfa production after corn. In ongoing field studies, we are evaluating whether plant growth regulators (PGR) may be used to aid the establishment of inters...

  10. [Population dynamics of ground carabid beetles and spiders in a wheat field along the wheat-alfalfa interface and their response to alfalfa mowing].

    Science.gov (United States)

    Liu, Wen-Hui; Hu, Yi-Jun; Hu, Wen-Chao; Hong, Bo; Guan, Xiao-Qing; Ma, Shi-Yu; He, Da-Han

    2014-09-01

    Taking the wheat-alfalfa and wheat-wheat interfaces as model systems, sampling points were set by the method of pitfall trapping in the wheat field at the distances of 3 m, 6 m, 9 m, 12 m, 15 m, 18 m, 21 m, 24 m, and 27 m from the interface. The species composition and abundance of ground carabid beetles and spiders captured in pitfalls were investigated. The results showed that, to some extent there was an edge effect on species diversity and abundance of ground carabid beetles and spiders along the two interfaces. A marked edge effect was observed between 15 m and 18 m along the alfalfa-wheat interface, while no edge effect was found at a distance over 20 m. The edge effect along the wheat-wheat interface was weaker in comparison to the alfalfa-wheat interface. Alfalfa mowing resulted in the migration of a large number of ground carabid beetles and spiders to the adjacent wheat filed. During ten days since mowing, both species and abundance of ground carabid beetles and spiders increased in wheat filed within the distance of 20 m along the alfalfa-wheat interface. The spatial distribution of species diversity of ground beetles and spiders, together with the population abundance of the dominant Chlaenius pallipes and Pardosa astrigera, were depicted, which could directly indicate the migrating process of natural enemy from alfalfa to wheat field.

  11. Beta-Amylases from Alfalfa (Medicago sativa L.) Roots.

    Science.gov (United States)

    Doehlert, D C; Duke, S H; Anderson, L

    1982-05-01

    Amylase was found in high activity (193 international units per milligram protein) in the tap root of alfalfa (Medicago sativa L. cv. Sonora). The activity was separated by gel filtration chromatography into two fractions with molecular weights of 65,700 (heavy amylase) and 41,700 (light amylase). Activity staining of electrophoretic gels indicated the presence of one isozyme in the heavy amylase fraction and two in the light amylase fraction. Three amylase isozymes with electrophoretic mobilities identical to those in the heavy and the light amylase fractions were the only amylases identified in crude root preparations. Both heavy and light amylases hydrolyzed amylopectin, soluble starch, and amylose but did not hydrolyze pullulan or beta-limit dextrin. The ratio of viscosity change to reducing power production during starch hydrolysis was identical for both alfalfa amylase fractions and sweet potato beta-amylase, while that of bacterial alpha-amylase was considerably higher. The identification of maltose and beta-limit dextrin as hydrolytic end-products confirmed that these alfalfa root amylases are all beta-amylases.The pH optimum for both beta-amylase fractions was 6.0. Both light and heavy beta-amylases showed normal Michaelis-Menten kinetics, with soluble starch as substrate, and had respectively K(m) values of 5.9 and 6.8 milligrams starch per milliliter and V(max) of 640 and 130 international units per milligram protein. Arrhenius plots indicated that the energy of activation for the heavy beta-amylase remained relatively unchanged (12.7 to 13.0 kilocalories per mole) from 0 to 30 degrees C, whereas the energy of activation for the light amylase increased from 12.0 to about 28.0 kilocalories per mole at 8.7 degrees C as temperature was lowered. The light amylase was shown to be inhibited by maltose.

  12. Identifying OH Imposters in the ALFALFA Neutral Hydrogen Survey

    Science.gov (United States)

    Suess, Katherine A.; Darling, Jeremy; Haynes, Martha P.; Giovanelli, Riccardo

    2016-06-01

    OH megamasers (OHMs) are rare, luminous molecular masers that are typically observed in (ultra) luminous infrared galaxies and serve as markers of major galaxy mergers. In blind emission line surveys such as the Arecibo Legacy Fast Arecibo L-Band Feed Array (ALFALFA) survey for neutral hydrogen (H I), OHMs at z ˜ 0.2 can mimic z ˜ 0.05 H I lines. We present the results of optical spectroscopy of ambiguous H I detections in the ALFALFA 40 per cent data release detected by the Wide Field Infrared Survey Explorer (WISE) but with uncertain optical counterparts. The optical redshifts, obtained from observations at the Apache Point Observatory, revealed five new OHMs and identified 129 H I optical counterparts. 60 candidates remain ambiguous. The new OHMs are the first detected in a blind spectral line survey. The number of OHMs in ALFALFA is consistent with predictions from the OH luminosity function. Additionally, the mid-infrared magnitudes and colours of the OHM host galaxies found in a blind survey do not seem to differ from those found in previous targeted surveys. This validates the methods used in previous IR-selected OHM surveys and indicates there is no previously unknown OHM-producing population at z ˜ 0.2. We also provide a method for future surveys to separate OH megamasers from 99 per cent of H I line emitters without optical spectroscopy by using WISE infrared colours and magnitudes. Since the fraction of OHMs found in flux-limited H I surveys is expected to increase with the survey's redshift, this selection method can be applied to future flux-limited high-redshift hydrogen surveys.

  13. Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa Callus

    Directory of Open Access Journals (Sweden)

    Ali A. Ehsanpour

    2005-01-01

    Full Text Available Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0, 15, 30 and 60 min and then, were transferred to MS medium containing 0, 2, 5, 10, 20, 30% PEG. All explants were kept in the dark in the culture room. Calluses tolerate to osmotic stress were selected according to fresh weight. Results showed that radiation of UV-C for 60 min increased the osmotic tolerance and decreased the effect of drought stress.

  14. China English, a Variety of English

    Institute of Scientific and Technical Information of China (English)

    吴卞

    2009-01-01

    With the economic globalization and the increasing interactiom between countries, English is more and more widely used as a means of international communication. It is not singly owned by one country or one nation, but has become an intermediate means of information. Inevitably, various varieties of English appeared as the result of the wide application of English. Theses varieties represent their own national and cultural characteristics. China English is one of these varieties. The paper makes an introduction to China English, analyses its characteristics through examples and points out the future development of China English.

  15. Star Formation in Undergraduate ALFALFA Team Galaxy Groups and Clusters

    Science.gov (United States)

    Koopmann, Rebecca A.; Durbala, Adriana; Finn, Rose; Haynes, Martha P.; Coble, Kimberly A.; Craig, David W.; Hoffman, G. Lyle; Miller, Brendan P.; Crone-Odekon, Mary; O'Donoghue, Aileen A.; Troischt, Parker; Undergraduate ALFALFA Team; ALFALFA Team

    2017-01-01

    The Undergraduate ALFALFA Team (UAT) Groups project is a coordinated study of gas and star formation properties of galaxies in and around 36 nearby (zwork undertaken by faculty and students at different institutions within the UAT. Here we present results from our wide area Hα and broadband R imaging project carried out with the WIYN 0.9m+MOSAIC/HDI at KPNO, including an analysis of radial star formation rates and extents of galaxies in the NGC 5846, Abell 779, NRGb331, and HCG 69 groups/clusters. This work has been supported by NSF grant AST-1211005 and AST-1637339.

  16. PERACETIC ACID PRETREATMENT OF ALFALFA STEM AND ASPEN BIOMASS

    OpenAIRE

    Lei Xu; Ulrike W. Tschirner

    2011-01-01

    Alfalfa stems and ground aspen were exposed to peracetic acid (0.5 to 9% on biomass) at temperatures ranging from 40 to 100° C and reaction times from 1 to 5 hours. Glucose release as a percentage of total cellulose content was determined using subsequent standard enzymatic hydrolysis. Statistical analysis confirmed that aspen showed a strong response to peracetic acid addition rate. 9% peracetic acid removed 14% of the original lignin and increased the rate of glucose release from 23% to 44%...

  17. Development of a modified independent parallel reactions kinetic model and comparison with the distributed activation energy model for the pyrolysis of a wide variety of biomass fuels.

    Science.gov (United States)

    Sfakiotakis, Stelios; Vamvuka, Despina

    2015-12-01

    The pyrolysis of six waste biomass samples was studied and the fuels were kinetically evaluated. A modified independent parallel reactions scheme (IPR) and a distributed activation energy model (DAEM) were developed and their validity was assessed and compared by checking their accuracy of fitting the experimental results, as well as their prediction capability in different experimental conditions. The pyrolysis experiments were carried out in a thermogravimetric analyzer and a fitting procedure, based on least squares minimization, was performed simultaneously at different experimental conditions. A modification of the IPR model, considering dependence of the pre-exponential factor on heating rate, was proved to give better fit results for the same number of tuned kinetic parameters, comparing to the known IPR model and very good prediction results for stepwise experiments. Fit of calculated data to the experimental ones using the developed DAEM model was also proved to be very good.

  18. EVALUATION OF THE FEED QUALITY OF FESTULOLIUM BRAUNII MIXTURES WITH MICROBIOLOGICALLY SUPPLIED RED CLOVER AND ALFALFA

    Directory of Open Access Journals (Sweden)

    Jacek Sosnowski

    2013-07-01

    Full Text Available The experience with cultivation of Festulolium braunii (Felopa variety in mixtures with red clover (Tenia variety, and alfalfa (Tula variety, was founded in April 2007 on an experimental object of Grassland Department and Green Areas Creation UP-H in Siedlce. The first experimental factor were 3-grass-legumes mixtures having the following composition: M1 – Festulolium braunii 50%, Trifolium pretense L. 50%, M2 – Festulolium braunii 50%, Medicago sativa ssp. media 50%, M3 – Festulolium braunii 50%, Trifolium pratense L. 25%, Medicago sativa ssp. media 25%. Combinations with soil’s medium amendment was marked as UG, and without soil’s medium amendment – BUG. In addition, nitrogen fertilization in the annual dose of 60 kg N∙ha-1, potassium 120 kg K2O∙ha-1 and phosphorus in the amount of 80 kg P2O5∙ha-1 were applied on all plots. Detailed study included the chemical composition of plant, which was determined at the Institute of Technology and Life Sciences in Falenty. The obtained results were used to calculate the following measures of the energy of and protein value feed: NEL – net energy of lactation, JPM∙kg-1D.M. – feed unit for milk production, JPŻ∙kg-1 D.M. – feed unit for livestock production, nBO – useful protein, BNŻ – rumen nitrogen balance. Furthermore, using the multivariate comparative of taxonomic analysis method the synthetic comparative measure of forage quality Q was evaluated. The use of soil’s medium amendment, regardless of the types of mixture, cuts and years of research, resulted in higher values of all measures. However, the analysis of synthetic measure of feed quality showed that in terms feed quality the best was the three component Festulolium braunii mixture with Trifolium pratense L. and Medicago sativa ssp. media.

  19. Assessment of stress tolerance, productivity, and forage quality in T1 transgenic alfalfa co-overexpressing ZxNHX and ZxVP1-1 from Zygophyllum xanthoxylum

    Directory of Open Access Journals (Sweden)

    Peng Kang

    2016-10-01

    Full Text Available Salinization, desertification, and soil nutrient deprivation are threatening the production of alfalfa (Medicago sativa L. in northern China. We have previously generated T0 transgenic alfalfa co-overexpressing Zygophyllum xanthoxylum ZxNHX and ZxVP1-1 genes with enhanced salt and drought tolerance. To further develop this excellent breeding material into the new forage cultivar, stress tolerance, productivity, and forage quality of T1 transgenic alfalfa (GM were assessed in this study. The GM inherited the traits of salt and drought tolerance from T0 generation. Most importantly, co-overexpression of ZxNHX and ZxVP1-1 enhanced the tolerance to Pi deficiency in GM, which was associated with more Pi accumulation in plants. Meanwhile, T1 transgenic alfalfa developed a larger root system with increased root size, root dry weight and root/shoot ratio, which may be one important reason for the improvement of phosphorus nutrition and high biomass accumulation in GM under various conditions. GM also accumulated more crude protein, crude fibre, crude fat, and crude ash than wild-type (WT plants, especially under stress conditions and in the field. More interestingly, the crude fat contents sharply dropped in WT (by 66%-74%, whereas showed no change or decreased less in GM, when subjected to salinity, drought or low-Pi. Our results indicate that T1 transgenic alfalfa co-overexpressing ZxNHX and ZxVP1-1 shows stronger stress tolerance, higher productivity and better forage quality. This study provides a solid foundation for creating the alfalfa cultivars with high yield, good quality and wide adaptability on saline, dry and nutrient-deprived marginal lands of northern China.

  20. Development of liquid chromatography methods coupled to mass spectrometry for the analysis of substances with a wide variety of polarity in meconium.

    Science.gov (United States)

    Meyer-Monath, Marie; Chatellier, Claudine; Cabooter, Deirdre; Rouget, Florence; Morel, Isabelle; Lestremau, Francois

    2015-06-01

    Meconium is the first fecal excretion of newborns. This complex accumulative matrix allows assessing the exposure of the fetus to xenobiotics during the last 6 months of pregnancy. To determine the eventual effect of fetal exposure to micropollutants in this matrix, robust and sensitive analytical methods must be developed. This article describes the method development of liquid chromatography methods coupled to triple quadrupole mass spectrometry for relevant pollutants. The 28 selected target compounds had different physico-chemical properties from very polar (glyphosate) to non-polar molecules (pyrethroids). Tests were performed with three different types of columns: reversed phase, ion exchange and HILIC. As a unique method could not be determined for the simultaneous analysis of all compounds, three columns were selected and suitable chromatographic methods were optimized. Similar results were noticed for the separation of the target compounds dissolved in either meconium extract or solvent for reversed phase and ion exchange columns. However, for HILIC, the matrix had a significant influence on the peak shape and robustness of the method. Finally, the analytical methods were applied to "real" meconium samples.

  1. Effect of UV-C on Drought Tolerance of Alfalfa (Medicago sativa) Callus

    OpenAIRE

    Ali A. Ehsanpour; Roya Razavizadeh

    2005-01-01

    Water stress and unfavorable climate decrease the growth and development of plant globally. Medicago is a valuable plant as a source of food for animal, foliage and for medicine. Since, Iran is located in a dry area; consequently, the study of plant responses to water stress is important. In this research UV-C radiation was used to induce physiological and genetic changes in alfalfa callus. In vitro grown calluses of M. sativa were exposed to UV-C for 0, 15, 30 and 60 min and then, were trans...

  2. Social Differences and English Varieties

    Institute of Scientific and Technical Information of China (English)

    Yang Jie

    2001-01-01

    Social varieties has long been taken into consideration, as people in different social group have different social manner and speech behavior. This paper focuses on several aspects, such as sex, age, social class, etc. Which account for language varieties in English. The purpose is to have a good understanding of learning English as second language.

  3. IDENTIFICATION OF SOIL FUNGI ISOLATED FROM ALFALFA (Medicago sativa L TO FIND SPECIFIC FUNGI WHICH IMPROVED THE GROWTH OF ALFALFA

    Directory of Open Access Journals (Sweden)

    T. Yudiarti

    2014-10-01

    Full Text Available Objective of the study was to identify all kinds of fungi which can life in the alfalfa plantation inBaturaden Purwokerto-Central Java. Fungi used in this study was 38 isolates. All fungi have been takenfrom the isolation of soil and root of diseased plant. Macroscopic and microscopic methods were usedfor identification. Potato Dextrose Agar (PDA medium was used to grow the fungi. All fungi wereidentified using book identification of fungi. The results showed that from 38 isolates, six species wasdetermined and one was unidentifed. Those species identified were Cuninghammela sp, Trichoderma sp,Vertilicium sp, Eupenicillium sp, Pythium sp, Aspergillus sp.

  4. The NSF Undergraduate ALFALFA Team: Partnering with Arecibo Observatory to Offer Undergraduate and Faculty Extragalactic Radio Astronomy Research Opportunities

    Science.gov (United States)

    Ribaudo, Joseph; Koopmann, Rebecca A.; Haynes, Martha P.; Balonek, Thomas J.; Cannon, John M.; Coble, Kimberly A.; Craig, David W.; Denn, Grant R.; Durbala, Adriana; Finn, Rose; Hallenbeck, Gregory L.; Hoffman, G. Lyle; Lebron, Mayra E.; Miller, Brendan P.; Crone-Odekon, Mary; O'Donoghue, Aileen A.; Olowin, Ronald Paul; Pantoja, Carmen; Pisano, Daniel J.; Rosenberg, Jessica L.; Troischt, Parker; Venkatesan, Aparna; Wilcots, Eric M.; ALFALFA Team

    2017-01-01

    The NSF-sponsored Undergraduate ALFALFA (Arecibo Legacy Fast ALFA) Team (UAT) is a consortium of 20 institutions across the US and Puerto Rico, founded to promote undergraduate research and faculty development within the extragalactic ALFALFA HI blind survey project and follow-up programs. The objective of the UAT is to provide opportunities for its members to develop expertise in the technical aspects of observational radio spectroscopy, its associated data analysis, and the motivating science. Partnering with Arecibo Observatory, the UAT has worked with more than 280 undergraduates and 26 faculty to date, offering 8 workshops onsite at Arecibo (148 undergraduates), observing runs at Arecibo (69 undergraduates), remote observing runs on campus, undergraduate research projects based on Arecibo science (120 academic year and 185 summer projects), and presentation of results at national meetings such as the AAS (at AAS229: Ball et al., Collova et al., Davis et al., Miazzo et al., Ruvolo et al, Singer et al., Cannon et al., Craig et al., Koopmann et al., O'Donoghue et al.). 40% of the students and 45% of the faculty participants have been women and members of underrepresented groups. More than 90% of student alumni are attending graduate school and/or pursuing a career in STEM. 42% of those pursuing graduate degrees in Physics or Astronomy are women.In this presentation, we summarize the UAT program and the current research efforts of UAT members based on Arecibo science, including multiwavelength followup observations of ALFALFA sources, the UAT Collaborative Groups Project, the Survey of HI in Extremely Low-mass Dwarfs (SHIELD), and the Arecibo Pisces-Perseus Supercluster Survey (APPSS). This work has been supported by NSF grants AST-0724918/0902211, AST-075267/0903394, AST-0725380, AST-121105, and AST-1637339.

  5. Cloning and characterization of a heme oxygenase-2 gene from alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Fu, Guang-Qing; Jin, Qi-Jiang; Lin, Yu-Ting; Feng, Jian-Fei; Nie, Li; Shen, Wen-Biao; Zheng, Tian-Qing

    2011-11-01

    Heme oxygenase (HO, EC 1.14.99.3) catalyzes the oxidation of heme and performs vital roles in plant development and stress responses. Two HO isozymes exist in plants. Between these, HO-1 is an oxidative stress-response protein, and HO-2 usually exhibited constitutive expression. Although alfalfa HO-1 gene (MsHO1) has been investigated previously, HO2 is still poorly understood. In this study, we report the cloning and characterization of HO2 gene, MsHO2, from alfalfa (Medica sativa L.). The full-length cDNA of MsHO2 contains an ORF of 870 bp and encodes for 290 amino acid residues with a predicted molecular mass of 33.3 kDa. Similar to MsHO1, MsHO2 also appears to have an N-terminal transit peptide sequence for chloroplast import. Many conserved residues in plant HO were also conserved in MsHO2. However, unlike HO-1, the conserved histidine (His) required for heme-iron binding and HO activity was replaced by tyrosine (Tyr) in MsHO2. Further biochemical activity analysis of purified mature MsHO2 showed no HO activity, suggesting that MsHO2 may not be a true HO in nature. Semi-quantitative RT-PCR confirmed its maximum expression in the germinating seeds. Importantly, the expression levels of MsHO2 were up-regulated under sodium nitroprusside (SNP) and H(2)O(2) (especially) treatment, respectively.

  6. The HI mass function in ALFALFA 70% and the role of confusion in future HI surveys

    Science.gov (United States)

    Jones, Michael G.; Papastergis, Emmanouil; Haynes, Martha P.; Giovanelli, Riccardo; ALFALFA Team

    2016-01-01

    The HI mass function represents a fundamental component of our understanding of the the gas content of galaxies. How its form varies throughout the local Universe and as a function of redshift, is key to developing a complete picture of galaxy evolution.We use the ALFALFA 70% catalog, the largest uniform catalog of extragalactic HI sources to date, to explore the environmental dependence of the HI mass function based on the projected neighbour densities in both SDSS and 2MRS. The Schechter function 'knee' mass is found to increase by approximately 0.2 dex from the lowest to highest density quartiles. However, this dependence is seen only when environment is defined by SDSS neighbours, and not with 2MRS. We interpret this as an indication of local, rather than large scale, environmental depedence. In addition, we find no evidence for any change in the faint-end slope; which is a source of tension with HI surveys of galaxy groups, and DM halo simulations.As HI surveys push deeper in an attempt to assess the HI content of the Universe towards a redshift of order unity, only the integral of the HI mass function will be measurable through stacking. We present an anaytic, and thus computationally cheap, method to estimate the contribution of HI confusion noise to these stacks and explore the design implications for upcoming SKA-precusor surveys.The ALFALFA team at Cornell is supported by NSF grants AST-0607007 and AST-1107390 to RG and MPH and by grants from the Brinson Foundation.

  7. Transgenic alfalfa plants expressing AtNDPK2 exhibit increased growth and tolerance to abiotic stresses.

    Science.gov (United States)

    Wang, Zhi; Li, Hongbing; Ke, Qingbo; Jeong, Jae Cheol; Lee, Haeng-Soon; Xu, Bingcheng; Deng, Xi-Ping; Lim, Yong Pyo; Kwak, Sang-Soo

    2014-11-01

    In this study, we generated and evaluated transgenic alfalfa plants (Medicago sativa L. cv. Xinjiang Daye) expressing the Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) gene under the control of the oxidative stress-inducible SWPA2 promoter (referred to as SN plants) to develop plants with enhanced tolerance to various abiotic stresses. We selected two SN plants (SN4 and SN7) according to the expression levels of AtNDPK2 and the enzyme activity of NDPK in response to methyl viologen (MV)-mediated oxidative stress treatment using leaf discs for further characterization. SN plants showed enhanced tolerance to high temperature, NaCl, and drought stress on the whole-plant level. When the plants were subjected to high temperature treatment (42 °C for 24 h), the non-transgenic (NT) plants were severely wilted, whereas the SN plants were not affected because they maintained high relative water and chlorophyll contents. The SN plants also showed significantly higher tolerance to 250 mM NaCl and water stress treatment than the NT plants. In addition, the SN plants exhibited better plant growth through increased expression of auxin-related indole acetic acid (IAA) genes (MsIAA3, MsIAA5, MsIAA6, MsIAA7, and MsIAA16) under normal growth conditions compared to NT plants. The results suggest that induced overexpression of AtNDPK2 in alfalfa will be useful for increasing biomass production under various abiotic stress conditions.

  8. Gas, Stars and Star Formation in ALFALFA Dwarf Galaxies

    CERN Document Server

    Huang, S; Giovanelli, R; Brinchmann, J; Stierwalt, S; Neff, S G

    2012-01-01

    We examine the global properties of the stellar and HI components of 229 low HI mass dwarf galaxies extracted from the ALFALFA survey, including a complete sample of 176 galaxies with HI masses < 10^{7.7} M_sun and HI line widths < 80 km s^{-1}. SDSS data are combined with photometric properties derived from GALEX to derive stellar masses (M_*) and star formation rates (SFRs) by fitting their UV-optical spectral energy distributions (SEDs). In optical images, many of the ALFALFA dwarfs are faint and of low surface brightness; only 56% of those within the SDSS footprint have a counterpart in the SDSS spectroscopic survey. A large fraction of the dwarfs have high specific star formation rates (SSFRs) and estimates of their SFRs and M_* obtained by SED fitting are systematically smaller than ones derived via standard formulae assuming a constant SFR. The increased dispersion of the SSFR distribution at M_* < 10^8 M_sun is driven by a set of dwarf galaxies that have low gas fractions and SSFRs; some of t...

  9. High-Throughput Development of SSR Markers from Pea (Pisum sativum L.) Based on Next Generation Sequencing of a Purified Chinese Commercial Variety.

    Science.gov (United States)

    Yang, Tao; Fang, Li; Zhang, Xiaoyan; Hu, Jinguo; Bao, Shiying; Hao, Junjie; Li, Ling; He, Yuhua; Jiang, Junye; Wang, Fang; Tian, Shufang; Zong, Xuxiao

    2015-01-01

    Pea (Pisum sativum L.) is an important food legume globally, and is the plant species that J.G. Mendel used to lay the foundation of modern genetics. However, genomics resources of pea are limited comparing to other crop species. Application of marker assisted selection (MAS) in pea breeding has lagged behind many other crops. Development of a large number of novel and reliable SSR (simple sequence repeat) or microsatellite markers will help both basic and applied genomics research of this crop. The Illumina HiSeq 2500 System was used to uncover 8,899 putative SSR containing sequences, and 3,275 non-redundant primers were designed to amplify these SSRs. Among the 1,644 SSRs that were randomly selected for primer validation, 841 yielded reliable amplifications of detectable polymorphisms among 24 genotypes of cultivated pea (Pisum sativum L.) and wild relatives (P. fulvum Sm.) originated from diverse geographical locations. The dataset indicated that the allele number per locus ranged from 2 to 10, and that the polymorphism information content (PIC) ranged from 0.08 to 0.82 with an average of 0.38. These 1,644 novel SSR markers were also tested for polymorphism between genotypes G0003973 and G0005527. Finally, 33 polymorphic SSR markers were anchored on the genetic linkage map of G0003973 × G0005527 F2 population.

  10. Albanese varieties of singular varieties over a perfect field

    CERN Document Server

    Russell, Henrik

    2011-01-01

    Let X be a projective variety, possibly singular. A generalized Albanese variety of X was constructed by Esnault, Srinivas and Viehweg over algebraically closed base field as a universal regular quotient of the relative Chow group of 0-cycles by Levine-Weibel. In this paper, we obtain a functorial description of the Albanese of Esnault-Srinivas-Viehweg over a perfect base field, using duality theory of 1-motives with unipotent part.

  11. In vivo Characterization of the Effects of Abscisic Acid and Drying Protocols Associated with the Acquisition of Desiccation Tolerance in Alfalfa (Medicago sativa L.) Somatic Embryos

    OpenAIRE

    SREEDHAR, LEKHA; Wolkers, Willem F.; Hoekstra, Folkert A.; BEWLEY, J. DEREK

    2002-01-01

    Although somatic embryos of alfalfa (Medicago sativa L.) had acquired some tolerance to desiccation at the cotyledonary stage of development (22 d after plating), additional culturing in 20 μm abscisic acid (ABA) for 8 d induced greater desiccation tolerance, as determined by increased germination. Compared with fast drying, slow drying of the ABA‐treated embryos improved desiccation tolerance. However, slow drying of non‐ABA‐treated embryos led to the complete loss of germination capacity, w...

  12. Homology theory on algebraic varieties

    CERN Document Server

    Wallace, Andrew H

    1958-01-01

    Homology Theory on Algebraic Varieties, Volume 6 deals with the principles of homology theory in algebraic geometry and includes the main theorems first formulated by Lefschetz, one of which is interpreted in terms of relative homology and another concerns the Poincaré formula. The actual details of the proofs of these theorems are introduced by geometrical descriptions, sometimes aided with diagrams. This book is comprised of eight chapters and begins with a discussion on linear sections of an algebraic variety, with emphasis on the fibring of a variety defined over the complex numbers. The n

  13. Stylistic Analysis on English Varieties

    Institute of Scientific and Technical Information of China (English)

    朱建丽

    2009-01-01

    There are primarily three factors influencing the stylistic varieties: field of discourse, mode of discourse, tenor of discourse. The varieties of social relations may directly work on the speaker' s phonetic, vocabulary, sentence pattern and etc. These varieties consist of formality, politeness, impersonality and accessibility. When a speaker convenes with different subjects in different occasions, the language he has chosen will vary greatly. Regarding this point, the author does not intend to provide the readers with accurate data. But a general analysis of legal documents and advertisements respectively on the following major levels.

  14. Relationship of Resistance to Meloidogyne chitwoodi (race 2) and M. hapla in Alfalfa

    OpenAIRE

    Per H McCord

    2012-01-01

    In the Pacific Northwest, alfalfa (Medicago sativa) is host to two species of root-knot nematodes, including race 2 of the Columbia root-knot nematode (Meloidogyne chitwoodi) and the northern root-knot nematode (Meloidogyne hapla). In addition to the damage caused to alfalfa itself by M. hapla, alfalfa’s host status to both species leaves large numbers of nematodes available to damage rotation crops, of which potato is the most important. A nematode-resistant alfalfa germplasm release, W12SR2...

  15. Evaluación cuantitativa de diez variedades de alfalfa (Medicago sativa L.).

    OpenAIRE

    Álvarez Vázquez, Perpetuo

    2013-01-01

    En México, la alfalfa es la leguminosa más utilizada en la alimentación del ganado lechero, debido a su alto rendimiento y valor nutritivo. El objetivo del presente estudio fue evaluar las variables que determinan el crecimiento y rendimiento de diez variedades de alfalfa a intervalos de corte definidos estacionalmente. El estudio se realizó de septiembre de 2011 a septiembre de 2012 en el Colegio de Postgraduados, Campus Montecillo, Texcoco, México. Las variedades de alfalfa estudiadas fuero...

  16. [Simulation alfalfa growth in Wulanbuhe sandy region].

    Science.gov (United States)

    Bai, Wenming; Bao, Xuemei

    2002-12-01

    Based on the theories of accumulated temperature and on the physio-ecological characteristics of Medicago sativa, a simulation model for its growth under soil water stress in arid sandy region was developed. The model was mainly composed of four modules: the stage module of growth, the dynamic module of leaf area index, the accumulated module of dry matter, and the distributive module of dry matter. After simulating and calculating, the model could be used to predict the growing progress and dynamic changes of leaf area and yield for herbage in sandy region. The result shows that the application of the model to production is usually effective.

  17. Small-scale spatial pattern of web-building spiders (Araneae) in alfalfa: relationship to disturbance from cutting, prey availability, and intraguild interactions.

    Science.gov (United States)

    Birkhofer, Klaus; Scheu, Stefan; Wise, David H

    2007-08-01

    Understanding the development of spatial patterns in generalist predators will improve our ability to incorporate them into biological control programs. We studied the small-scale spatial patterns of spider webs in alfalfa by analyzing the relationship between web locations over distances ranging from 4 to 66 cm. Using a coordinate-based spatial statistic (O-ring) and assuming a heterogeneous distribution of suitable web sites, we analyzed the impact of cutting and changes in spider abundance on web distribution. We analyzed the influence of small-scale variation in prey availability by comparing web distributions to the pattern of sticky-trap captures of Aphididae and Diptera described by a count-based spatial statistic (SADIE). Cutting of alfalfa reduced the overall density of web-building spiders but had no immediate impact on the spatial distribution of their webs. Availability of aphids was highest before the alfalfa was cut and was clumped at a scale of 66 cm. Spider webs, however, were not clumped at any scale or date. In contrast, webs were regularly distributed at smaller distances (web-building spiders were most active during this period, we hypothesize that the development of small-scale regularity in web locations was driven by intraguild interactions. Our results suggest that intraguild interactions contribute to the development of small-scale spatial patterns of spider webs in alfalfa. Variation in prey availability may have more of an influence on web distribution in crops with a different vegetation structure or if patterns are studied at larger spatial scales.

  18. Prym varieties of spectral covers

    CERN Document Server

    Hausel, Tamás

    2010-01-01

    Given a possibly reducible and non-reduced spectral cover X over a smooth projective complex curve C we determine the group of connected components of the Prym variety Prym(X/C). We also describe the sublocus of characteristics a for which the Prym variety Prym(X_a/C) is connected. These results extend special cases of work of Ng\\^o who considered integral spectral curves.

  19. Alfalfa microsymbionts from different ITS and nodC lineages of Ensifer meliloti and Ensifer medicae symbiovar meliloti establish efficient symbiosis with alfalfa in Spanish acid soils.

    Science.gov (United States)

    Ramírez-Bahena, Martha-Helena; Vargas, Margarita; Martín, María; Tejedor, Carmen; Velázquez, Encarna; Peix, Álvaro

    2015-06-01

    Alfalfa (Medicago sativa L.) is an important crop worldwide whose cropping in acid soils is hampered by the poor nodulation and yield commonly attributed to the sensitivity of its endosymbionts to acid pH. In this work, we isolated several acid-tolerant strains from alfalfa nodules in three acid soils in northwestern Spain. After grouping by RAPD fingerprinting, most strains were identified as Ensifer meliloti and only two strains as Ensifer medicae according to their 16S-23S intergenic spacer (ITS) sequences that allowed the differentiation of two groups within each one of these species. The two ITS groups of E. meliloti and the ITS group I of E. medicae have been previously found in Medicago nodules; however, the group II of E. medicae has been only found to date in Prosopis alba nodules. The analysis of the nodC gene showed that all strains isolated in this study belong to the symbiovar meliloti, grouping with the type strains of E. meliloti or E. medicae, but some harboured nodC gene alleles different from those found to date in alfalfa nodules. The strains of E. medicae belong to the symbiovar meliloti which should be also recognised in this species, although they harboured a nodC allele phylogenetically divergent to those from E. meliloti strains. Microcosm experiments showed that inoculation of alfalfa with selected acid-tolerant strains significantly increased yields in acid soils representing a suitable agricultural practice for alfalfa cropping in these soils.

  20. Studies on the Alfalfa Growing Effects of Alfalfa Rhizobium%苜蓿根瘤菌剂对紫花苜蓿生长影响研究

    Institute of Scientific and Technical Information of China (English)

    樊川; 杨旭升; 曹亚斌

    2015-01-01

    通过不同剂量苜蓿根瘤菌接种两种紫花苜蓿试验,比较了接种根瘤菌剂对紫花苜蓿生长的影响。试验结果表明:接种苜蓿根瘤菌剂能够提高紫花苜蓿结瘤数量和根瘤重,促进紫花苜蓿的生长发育,增加苜蓿草的产草量。在所试验的接种剂量中,以每 kg 苜蓿种子接种12mL 根瘤菌剂为最佳接种量。%This paper studied the alfalfa growing effects of alfalfa rhizobium by inoculated different amounts.The experimental results showed that inoculating rhizobium can raise the nodulating amount of alfalfa and the weight of nodulations,promoting the growth of alfalfas and augment the productions of alfalfas.Among the different inoculated amounts,the amount of 12ml is the best inoculated amount.

  1. PENINGKATAN PRODUKSI DAN KECERNAAN BAHAN KERING ALFALFA DENGAN PEMUPUKAN FOSFAT

    Directory of Open Access Journals (Sweden)

    - Sunarni

    2014-06-01

    Full Text Available Alfalfa (Medicago    sativa    L. as one of legume forage crops for ruminant livestock is very sensitive to the phosphorus deficiency. Phosphorus fertilizers have been used widely to overcome those problem. However, the high cost of superphosphate (SP is now focusing attention on cheaper rock phosphate (RP fertilizer. A field experiment was conducted on a latosolic soil (low pH, and low available Bray II extractable P. The objective of the research is to evaluate the effect of phosphorus fertilizer from difference sources on dry matter production, and in    vitro dry matter digestibility of alfalfa. Completely randomized block design with 7 treatments in 3 replicates was used in this field experiment. The treatments were T0 (control, T1 (SP, 100 kg P2O5/ha, T2 (SP, 200 kg P2O5/ha, T3 (SP, 300 kg P2O5/ha, T4 (RP, 100 kg P2O5/ha, T5 (RP, 200 kg P2O5/ha, T6 (RP, 300 kg P2O5/ha. Size of each plot was 2 m x 2 m, and fertilized with P fertilizer (SP, RP according to the assigned treatment. All plot received basal fertilizer of urea (50 kg N/ha, KCl (100 kg K2O/ha and poultry manure (2 ton/ha. Medicago    sativa was defoliated on 12 weeks after planting and analyzed for dry matter (DM production and in    vitro DM digestibility. Result showed that DM production significantly influenced by the treatments. Superphosphate fertilization resulted DM production significantly higher compared to control and RP, except on RP 300 kg P2O5/ha was non-significant difference. In    vitro dry matter digestibility (IVDMD of alfalfa was not affected by the treatment. There was non-significant difference in IVDMD between the treatments. Therefore, RP (300 kg P2O5/ha could replace SP to increased DM production and not affected for IVDMD of Medicago    sativa in acid latosolic soil.

  2. Effects of chlorpyrifos and chlorantraniliprole on fermentation quality of alfalfa (Medicago sativa L.) silage inoculated with or without Lactobacillus plantarum LP.

    Science.gov (United States)

    Zhang, Qing; Yu, Zhu; Wang, Xianguo; Na, Risu

    2017-03-01

    The effects of pesticides and Lactobacillus plantarum (LP) on fermentation quality of alfalfa (Medicago sativa L.) silage were investigated. Chlorpyrifos and chlorantraniliprole were sprayed on the surface of alfalfa plants at 658.6 and 45.0 g active ingredient/ha, respectively. Alfalfa plants were harvested on day 5 post-application and ensiled with or without LP. Chlorpyrifos and chlorantraniliprole decreased the yeast count of alfalfa material (P alfalfa silage (P alfalfa silage treated with pesticides (P alfalfa silage (P alfalfa silage and affected the fermentation process, whereas LP improved the fermentation quality of pesticides-contaminated alfalfa silage and slowed down the dissipation of chlorpyrifos.

  3. Mezclas de Alfalfa y Gramíneas en la Serie Sabana de Bogotá Tipo Franco Arcilloso.

    Directory of Open Access Journals (Sweden)

    Chaverra Gil. Hernán

    1960-06-01

    Full Text Available En el Centro Nacional de Investigaciones Agrícolas, Tibaitatá, y en la serie de suelos Sabana de Bogotá, tipo frnaco arcilloso, se llevaron a cabo dos experimentos con mezclas de alfalfa y gramíneas. En el primer ensayo, se sembraron simultáneamente con la alfalfa, ryegrass inglés, rygrass anual y orchoso. Los métodos de siembra utilizados fueron: a alfalfa y gramíneas sembradas al voleo, b alfalfa y gramíneas en el mismo surco, c alfalfa y gramíneas en surcos alternos, d alfalfa en surcos y gramíneas al voleo y e alfalfa en surcos y trébol blanco al voleo. En el segundo experimento se sembró la alfalfa en surcos separados 25 cm. Después de establecida la alfalfa e inmediatamente después del 4° corte se sembraron al voleo las siguientes gramíneas: ryegrass inglés, rygrass anual, orchoro, festuca media, pasto cinta y pasto rescate. Las finalidades del estudio fueron las de valorar el comportamiento de las gramíneas en mezcla con la alfalfa, mediante la determinación progresiva de la composición botánica y el rendimiento de forraje seco de las mezclas. La siembra simultánea de ryegrass inglés y ryegrass anual con la alfalfa dio como resultado una mala población de la leguminosa, especialmente en los dos primeros cortes. La competencia ejercida por estas dos gramíneas a la alfalfa, se redujo casi completamente, cuando se sembraron en surcos alternos o en la alfalfa establecida. Sea cual fuere el pasto sembrado, la siembra de gramíneas en alfalfa establecida, redujo la competencia entre los pastos asociados. En este ensayo el factor más importante en el establecimiento de las gramíneas, fue el agua disponible en el suelo. Bajo las condiciones del primer experimento el orchoro fue la gramínea que mejor creció en asocio de la alfalfa en todos los métodos de siembra estudiado. La producción promedia de la mezcla varió entre 2600 y 3145 Kg/hect. De forreje seco por corte y la proporción de orchoro entre

  4. The use of near-infrared reflectance spectroscopy (NIRS in the prediction of chemical composition and in vitro neutral detergent fiber (NDF digestibility of Italian alfalfa hay

    Directory of Open Access Journals (Sweden)

    Andrea Formigoni

    2010-01-01

    Full Text Available The objective of the present work was to develop calibration equations for the prediction of chemical composition and forage digestibility from different populations of alfalfa hay harvested in Parmigiano- Reggiano cheese production area of Northern Italy. Due to annual climatic variations that affect soil and growing conditions and consequently cause high variability in chemical and physical composition, 319 hay samples from four years were used to build robust calibration. NIRS calibration equations were developed for the prediction of in vitro NDF digestibility (IVNDFd and 20 chemical parameters (dry matter, starch, fat, sugar, fiber fractions, crude protein, nitrogen fractions and some minerals of Italian hay. The results obtained show that NIRS equations greatly explain the variation in the composition existing in alfalfa hays grown in Northern Italy, except for a few parameters characterized by low variability range value. The equations obtained for the prediction of biological and chemical parameters explain the major part of the variation existing in the reference data, which open great prospects for the use of NIRS in planning feeding strategies of livestock, based on alfalfa forage.

  5. Phosphate Rock Application on Alfalfa (Medicago sativa L. Production and Macronutrients in Latosol Soil

    Directory of Open Access Journals (Sweden)

    Y. Liani

    2011-12-01

    Full Text Available The objective of this study was to evaluate the effectiveness of phosphate rocks (PRs fertilizer compared to chemical P fertilizer for the best crop production and macronutrients of alfalfa. A completely randomized design under 3x3 factorial patterns was used in this research. The first factor was different sources of P fertilizer: Guizhou Phosphate Rock (GPR, Jingxiang Phosphate Rock (JPR, and Single Super Phosphate (SSP. The second factor was level of P fertilizer: 75, 100, and 125 mg P2O5/kg soil. A control treatment (without addition of P fertilizer, CK was added as a comparison with the treatments. The results showed that JPR was the best for alfalfa production, whereas GPR and SSP were better for nutrient content in the alfalfa tissue than JPR. On the whole, phosphate rocks had similar effect on alfalfa growth compared to SSP at the experimental conditions.

  6. HI Gas in Early Type Galaxies as Measured by ALFALFA

    Science.gov (United States)

    Collins, Wendy; Morrison, Ryan; Green, Jarred; Raskin, Mark; Crawford, Connor; Bomer-Lawson, August; Hannan, Joshua; Crone-Odekon, Mary; ALFALFA Team

    2016-01-01

    We present the HI content of 1580 early type galaxies (ETGs) in a total sample of 7747 galaxies that have HI measurements or upper limits from the ALFALFA survey. We find a significant correlation between HI content and local density, with HI detections almost exclusively in low-density environments. Using optical line ratios, we split the population into galaxies with spectral lines dominated by active galactic nuclei (AGN) and dominated by star forming regions. Compared with HI-rich star forming ETGs, HI-rich ETGs with AGN tend to be brighter and redder and to exhibit a stronger correlation between stellar mass and HI mass. This work is supported by NSF grant AST-1211005.

  7. Role of Silicon Counteracting Cadmium Toxicity in Alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Kabir, Ahmad H; Hossain, Mohammad M; Khatun, Most A; Mandal, Abul; Haider, Syed A

    2016-01-01

    Cadmium (Cd) is one of the most phytotoxic elements causing an agricultural problem and human health hazards. This work investigates whether and how silicon (Si) ameliorates Cd toxicity in Alfalfa. The addition of Si in Cd-stressed plants caused significant improvement in morpho-physiological features as well as total protein and membrane stability, indicating that Si does have critical roles in Cd detoxification in Alfalfa. Furthermore, Si supplementation in Cd-stressed plants showed a significant decrease in Cd and Fe concentrations in both roots and shoots compared with Cd-stressed plants, revealing that Si-mediated tolerance to Cd stress is associated with Cd inhibition in Alfalfa. Results also showed no significant changes in the expression of two metal chelators [MsPCS1 (phytochelatin synthase) and MsMT2 (metallothionein)] and PC (phytochelatin) accumulation, indicating that there may be no metal sequestration or change in metal sequestration following Si application under Cd stress in Alfalfa. We further performed a targeted study on the effect of Si on Fe uptake mechanisms. We observed the consistent reduction in Fe reductase activity, expression of Fe-related genes [MsIRT1 (Fe transporter), MsNramp1 (metal transporter) and OsFRO1 (ferric chelate reductase] and Fe chelators (citrate and malate) by Si application to Cd stress in roots of Alfalfa. These results support that limiting Fe uptake through the down-regulation of Fe acquisition mechanisms confers Si-mediated alleviation of Cd toxicity in Alfalfa. Finally, an increase of catalase, ascorbate peroxidase, and superoxide dismutase activities along with elevated methionine and proline subjected to Si application might play roles, at least in part, to reduce H2O2 and to provide antioxidant defense against Cd stress in Alfalfa. The study shows evidence of the effect of Si on alleviating Cd toxicity in Alfalfa and can be further extended for phytoremediation of Cd toxicity in plants.

  8. HI Gas in Large-Scale Filaments as Measured by ALFALFA

    Science.gov (United States)

    Elliott, Skye; Phi, An; Shah, Ebrahim; Livecchi, Jack; Yu, Yang; Gengras, Graeme; Wolfe, Pierre-Francois; Crone-Odekon, Mary; Hyman, Mario; ALFALFA Team

    2016-01-01

    We assess the relationship between galaxy environment and HI content as measured by ALFALFA. In particular, we consider membership in large-scale filaments in order to provide clues to how star formation in galaxies is quenched in different environments. We use data from the Sloan Digital Sky Survey to define galaxy environments in terms of clusters, filaments, and voids for a sample of galaxies with z ALFALFA. This work is supported by NSF grant AST-1211005.

  9. Role of Silicon Counteracting Cadmium Toxicity in Alfalfa (Medicago sativa L.)

    Science.gov (United States)

    Kabir, Ahmad H.; Hossain, Mohammad M.; Khatun, Most A.; Mandal, Abul; Haider, Syed A.

    2016-01-01

    Cadmium (Cd) is one of the most phytotoxic elements causing an agricultural problem and human health hazards. This work investigates whether and how silicon (Si) ameliorates Cd toxicity in Alfalfa. The addition of Si in Cd-stressed plants caused significant improvement in morpho-physiological features as well as total protein and membrane stability, indicating that Si does have critical roles in Cd detoxification in Alfalfa. Furthermore, Si supplementation in Cd-stressed plants showed a significant decrease in Cd and Fe concentrations in both roots and shoots compared with Cd-stressed plants, revealing that Si-mediated tolerance to Cd stress is associated with Cd inhibition in Alfalfa. Results also showed no significant changes in the expression of two metal chelators [MsPCS1 (phytochelatin synthase) and MsMT2 (metallothionein)] and PC (phytochelatin) accumulation, indicating that there may be no metal sequestration or change in metal sequestration following Si application under Cd stress in Alfalfa. We further performed a targeted study on the effect of Si on Fe uptake mechanisms. We observed the consistent reduction in Fe reductase activity, expression of Fe-related genes [MsIRT1 (Fe transporter), MsNramp1 (metal transporter) and OsFRO1 (ferric chelate reductase] and Fe chelators (citrate and malate) by Si application to Cd stress in roots of Alfalfa. These results support that limiting Fe uptake through the down-regulation of Fe acquisition mechanisms confers Si-mediated alleviation of Cd toxicity in Alfalfa. Finally, an increase of catalase, ascorbate peroxidase, and superoxide dismutase activities along with elevated methionine and proline subjected to Si application might play roles, at least in part, to reduce H2O2 and to provide antioxidant defense against Cd stress in Alfalfa. The study shows evidence of the effect of Si on alleviating Cd toxicity in Alfalfa and can be further extended for phytoremediation of Cd toxicity in plants. PMID:27512401

  10. Role of silicon counteracting cadmium toxicity in Alfalfa (Medicago sativa L.

    Directory of Open Access Journals (Sweden)

    Ahmad H. Kabir

    2016-07-01

    Full Text Available Cadmium (Cd is one of the most phytotoxic elements causing an agricultural problem and human health hazards. This work investigates whether and how silicon (Si ameliorates Cd toxicity in Alfalfa. The addition of Si in Cd-stressed plants caused significant improvement in morpho-physiological features as well as total protein and membrane stability, indicating that Si does have critical roles in Cd detoxification in Alfalfa. Furthermore, Si supplementation in Cd-stressed plants showed a significant decrease in Cd and Fe concentrations in both roots and shoots compared with Cd-stressed plants, revealing that Si-mediated tolerance to Cd stress is associated with Cd inhibition in Alfalfa. Results also showed no significant changes in the expression of two metal chelators [MsPCS1 (phytochelatin synthase and MsMT2 (metallothionein] and PC (phytochelatin accumulation, indicating that there may be no metal sequestration or change in metal sequestration following Si application under Cd stress in Alfalfa. We further performed a targeted study on the effect of Si on Fe uptake mechanisms. We observed the consistent reduction in Fe reductase activity, expression of Fe-related genes [MsIRT1 (Fe transporter, MsNramp1 (metal transporter and OsFRO1 (ferric chelate reductase] and Fe chelators (citrate and malate by Si application to Cd stress in roots of Alfalfa. These results support that limiting Fe uptake through the down-regulation of Fe acquisition mechanisms confers Si-mediated alleviation of Cd toxicity in Alfalfa. Finally, an increase of catalase (CAT, ascorbate peroxidase (APX and superoxide dismutase (SOD activities along with elevated methionine and proline subjected to Si application might play roles, at least in part, to reduce H2O2 and to provide antioxidant defense against Cd stress in Alfalfa. The study shows evidence of the effect of Si on alleviating Cd toxicity in Alfalfa and can be further extended for phytoremediation of Cd toxicity in plants.

  11. The HI Content of Groups as Measured by ALFALFA

    Science.gov (United States)

    Koopmann, Rebecca A.; Crone-Odekon, Mary; Haynes, Martha P.; Finn, Rose; Hallenbeck, Gregory L.; Giovanelli, Riccardo; ALFALFA Team

    2016-01-01

    We present the HI content of galaxies in nearby groups and clusters as measured by the 70% complete Arecibo Legacy Fast-ALFA (ALFALFA) survey, including constraints from ALFALFA detection limits. Our sample includes 22 groups at distances between 70-160 Mpc over the mass range 12.5 < log M/M⊙ < 15.0, for a total of 1986 late-type galaxies out to a projected group-centric distance of 4.0 Mpc. We find that late-type galaxies in the centers of groups lack HI at fixed stellar mass relative to the regions surrounding them. Larger groups show evidence of a stronger gradient in HI properties, despite a similar gradient in stellar mass, and in color at fixed stellar mass, over the same range in r/R200. We compare several environment variables to determine which is the best predictor of galaxy properties; group-centric distance r and r/R200 are similarly effective predictors, while local density is slightly more effective and group size and halo mass are slightly less effective. Finally, we see evidence that HI is deficient for blue cloud galaxies in denser environments even when both stellar mass and color are fixed. This is consistent with a picture where HI is removed or destroyed, followed by reddening within the blue cloud. Overall, our results support the existence of pre-processing in isolated groups, along with an additional rapid mechanism for gas removal within larger groups and clusters, perhaps by ram-pressure stripping. This work has been supported by NSF grants AST-0724918/0902211, AST-075267/0903394, AST-0725380, and AST-1211005.

  12. ALFALFA Hα Reveals How Galaxies Use Their Hi Fuel

    Science.gov (United States)

    Jaskot, Anne; Oey, Sally; Salzer, John; van Sistine, Angie; Bell, Eric; Haynes, Martha

    Atomic hydrogen traces the raw material from which molecular clouds and stars form. With 565 galaxies from the ALFALFA Hα survey, a statistically complete subset of the ALFALFA survey, we examine the processes that affect galaxies' abilities to access and consume their Hi gas. On galaxy-wide scales, Hi gas fractions correlate only weakly with instantaneous specific star formation rates (sSFRs) but tightly with galaxy color. We show that a connection between dust and Hi content, arising from the fundamental mass-metallicity-Hi relation, leads to this tight color correlation. We find that disk galaxies follow a relation between stellar surface density and Hi depletion time, consistent with a scenario in which higher mid-plane pressure leads to more efficient molecular cloud formation from Hi. In contrast, spheroids show no such trend. Starbursts, identified by Hα equivalent width, do not show enhanced Hi gas fractions relative to similar mass non-starburst galaxies. The starbursts' shorter Hi depletion times indicate more efficient consumption of Hi, and galaxy interactions drive this enhanced star formation efficiency in several starbursts. Interestingly, the most disturbed starbursts show greater enhancements in Hi gas fraction, which may indicate an excess of Hi at early merger stages. At low galaxy stellar masses, the triggering mechanism for starbursts is less clear; the high scatter in efficiency and sSFR among low-mass galaxies may result from periodic bursts. We find no evidence for depleted Hi reservoirs in starbursts, which suggests that galaxies may maintain sufficient Hi to fuel multiple starburst episodes.

  13. Alimentos funcionales, alfalfa y fitoestrógenos

    Directory of Open Access Journals (Sweden)

    Alejandro De Jesús

    2016-05-01

    Full Text Available El desarrollo de alimentos funcionales en los últimos años ha ido en aumento debido principalmente, al interés de la población por alimentos que no solo cumplan con la función nutricional sino también por que tengan un efecto benéfico en salud reduciendo así el riesgo a padecer diversas enfermedades. La alfalfa ha sido generalmente un alimento destinado para la alimentación del ganado, sin embargo, en diversas investigaciones alrededor del mundo se ha reportado que contiene compuestos bioactivos –fitoquímicos– y proteínas con efecto estrogénico, antimicrobiano y antioxidante, así como también protector contra enfermedades cardiovasculares, cáncer y diabetes, entre otras, clasificándola potencialmente como un alimento funcional. El presente documento tiene como objetivo dar un bosquejo general informativo a través de la búsqueda y revisión bibliográfica acerca de los alimentos funcionales y en especial de la alfalfa y constituyentes fitoquímicos (fitoestrógenos que la ubican dentro de este grupo de alimentos; así como los hallazgos que se han reportado como efectos adversos en animales experimentales específicamente en el aparato reproductivo debido al consumo de este tipo de fitocompuestos. Esta dicotomía entre los efectos adversos y benéficos no es más que la señalización de que aún falta mucha más información y estudios que realizar respecto al resultado que pueden tener los fitoestrógenos en el desarrollo y función reproductiva de animales, el agonismo y antagonismo de su efecto así como la concordancia en el riesgo-beneficio de su consumo.

  14. Salt stress alters DNA methylation levels in alfalfa (Medicago spp).

    Science.gov (United States)

    Al-Lawati, A; Al-Bahry, S; Victor, R; Al-Lawati, A H; Yaish, M W

    2016-02-26

    Modification of DNA methylation status is one of the mechanisms used by plants to adjust gene expression at both the transcriptional and posttranscriptional levels when plants are exposed to suboptimal conditions. Under abiotic stress, different cultivars often show heritable phenotypic variation accompanied by epigenetic polymorphisms at the DNA methylation level. This variation may provide the raw materials for plant breeding programs that aim to enhance abiotic stress tolerance, including salt tolerance. In this study, methylation-sensitive amplified polymorphism (MSAP) analysis was used to assess cytosine methylation levels in alfalfa (Medicago spp) roots exposed to increasing NaCl concentrations (0.0, 8.0, 12.0, and 20.0 dS/m). Eleven indigenous landraces were analyzed, in addition to a salt-tolerant cultivar that was used as a control. There was a slight increase in DNA methylation upon exposure to high levels of soil salinity. Phylogenetic analysis using MSAP showed epigenetic variation within and between the alfalfa landraces when exposed to saline conditions. Based on MSAP and enzyme-linked immunosorbent assay results, we found that salinity increased global DNA methylation status, particularly in plants exposed to the highest level of salinity (20 dS/m). Quantitative reverse transcription-polymerase chain reaction indicated that this might be mediated by the overexpression of methyltransferase homolog genes after exposure to saline conditions. DNA demethylation using 5-azacytidine reduced seedling lengths and dry and fresh weights, indicating a possible decrease in salinity tolerance. These results suggest that salinity affects DNA methylation flexibility.

  15. Preference mapping of apple varieties in Europe

    NARCIS (Netherlands)

    Bonany, J.; Buehler, A.; Carbó, J.; Codarin, C.; Donati, F.; Echeverria, G.; Egger, S.; Guerra, W.; Hilaire, C.; Höller, I.; Iglesias, I.; Jesionkowska, K.; Konopacka, D.; Kruczynska, D.; Martinelli, A.; PItiot, C.; Sansavini, S.; Stehr, R.; Schoorl, F.W.

    2014-01-01

    A consumer test carried out in 7 different European countries compared 3 standard apple varieties to 8 new ones. A total of 4290 consumers took part in the test. Data from this test was used to develop a preference map for apple. The preference map was constructed with 3 main dimensions (1 – sweetne

  16. The Varieties of Good Design

    DEFF Research Database (Denmark)

    Ylirisku, Salu; Arvola, Mattias

    2016-01-01

    This chapter explores the philosopher and logician Georg Henrik von Wright’s metaethical treatise of the varieties of goodness in the context of design. von Wright investigated the use of notion of ‘good’ in language, and he identified six kinds of goodness: namely utilitarian goodness, instrumen......This chapter explores the philosopher and logician Georg Henrik von Wright’s metaethical treatise of the varieties of goodness in the context of design. von Wright investigated the use of notion of ‘good’ in language, and he identified six kinds of goodness: namely utilitarian goodness...

  17. Schubert unions in Grassman varieties

    DEFF Research Database (Denmark)

    Hansen, Johan Peder; Johnsen, Trygve; Ranestad, Kristian

    2007-01-01

    , their number of Fq -rational points. Moreover, we study a geometric duality of such unions, and give a combinatorial interpretation of this duality. We discuss the maximum number of Fq -rational points for Schubert unions of a given spanning dimension, and as an application to coding theory, we study......We study subsets of Grassmann varieties G(l,m) over a field F, such that these subsets are unions of Schubert cycles, with respect to a fixed flag. We study unions of Schubert cycles of Grassmann varieties G(l,m) over a field F. We compute their linear span and, in positive characteristic...

  18. In situ phytoremediation of PAH-contaminated soil by intercropping alfalfa (Medicago sativa L.) with tall fescue (Festuca arundinacea Schreb.) and associated soil microbial activity

    Energy Technology Data Exchange (ETDEWEB)

    Sun, Mingming; Fu, Dengqiang; Teng, Ying; Shen, Yuanyuan; Luo, Yongming; Li, Zhengao [Chinese Academy of Sciences, Nanjing (China). Key Laboratory of Soil Environment and Pollution Remediation; Christie, Peter [Agri-Food and Biosciences Institute, Belfast (United Kingdom). Agri-Environment Branch

    2011-09-15

    Purpose: A 7-month field experiment was conducted to investigate the polycyclic aromatic hydrocarbon (PAH) remediation potential of two plant species and changes in counts of soil PAH-degrading bacteria and microbial activity. Materials and methods: Alfalfa and tall fescue were grown in monoculture and intercropped for 7 months in contaminated field soil. Soil and plant samples were analyzed for PAHs. Plant biomass, densities of PAH-degradation soil bacteria, soil microbial biomass C and N, enzyme activities, and the physiological profile of the soil microbial community were determined. Results and discussion: Average removal percentage of total PAHs in intercropping (30.5%) was significantly higher than in monoculture (19.9%) or unplanted soil (-0.6%). About 7.5% of 3-ring, 12.3% of 4-ring, and 17.2% of 5(+6)-ring PAHs were removed from the soil by alfalfa, with corresponding values of 25.1%, 10.4%, and 30.1% for tall fescue. Intercropping significantly enhanced the remediation efficiency. About 18.9% of 3-ring, 30.9% of 4-ring, and 33.4% of 5(+6)-ring PAHs were removed by the intercropping system. Higher counts of soil culturable PAH-degrading bacteria and elevated microbial biomass and enzyme activities were found after intercropping. Soil from intercropping showed significantly higher (p < 0.05) average well-color development obtained by the BIOLOG Ecoplate assay and Shannon-Weaver index compared with monoculture. Conclusions: Cropping promoted the dissipation of soil PAHs. Tall fescue gave greater removal of soil PAHs than alfalfa, and intercropping was more effective than monoculture. Intercropping of alfalfa and tall fescue may be a promising in situ bioremediation strategy for PAH-contaminated soils. (orig.)

  19. 紫云英SSR分子标记的开发及在品种鉴别中的应用%Development of Microsatellite Markers for Astragalus sinicus L.and Its Application for Variety Identification

    Institute of Scientific and Technical Information of China (English)

    陈坚; 张辉; 朱炳耀; 林新坚

    2011-01-01

    利用生物素标记的(AG)15、(CT)1s、(AC)15、(GT)15探针及链霉素亲和磁珠,从紫云英的基因组中富集微卫星(SSR)序列.在用富集片段构建插入文库的950个转化子中,经PCR检测及测序共得到127个SSR序列,微卫星序列的富集效率达15.8%.除去重复或无效的序列,得到33个序列用于引物设计.对征集的9个紫云英品种进行多态性分析,有6对引物扩增出明显且稳定的多态性位点18个.在紫云英的品种水平上,这些SSR位点产生的多态率为50%~100%,有效等位基因数为1.19~1.64,Nei氏遗传多样性为0.13~0.38,Shannon多态信息指数为0.22~0.56;利用这6对引物可将参试品种完全区分开,证明这些SSR位点可用于紫云英品种的指纹鉴别.根据SSR位点的相似性系数,将9个紫云英品种主要聚成两个类群,与传统按生育期的紫云英品种划分结果无必然的联系.%Astragalus sinicus L. Is a traditional green manure used in rice field in China. To identify Astragalus sinicus L. Varieties in agricultural production, we developed SSR molecular markers by using biotinylated (AG)15, (CT)15, (AC)15, (GT)15 probes and streptavidin coated magnetic beads, the SSR sequences were enriched from A. Sinicus genome. Of the 950 transformants selected from the library constructed by obtained fragments, 127 clones were confirmed as SSR insert by PCR identification and final sequencing, with a SSR enrichment efficiency of 15.8%. By deleting the duplicated and unavailable sequences, 33 SSR loci were used for primer design. After nine A. Sinicus varieties were sampled for analysis of the genetic diversity based on these SSR primers, only six SSR primer pairs amplified a total of 18 stable and repeatable polymorphic bands within tested plant samples. At the level of variety population of A. Sinicus, based on these SSR loci we calculated the percentage of polymorphic band ranging from 50% to 100%, effective number of alleles ranging from

  20. Overexpression of Medicago sativa TMT elevates the α-tocopherol content in Arabidopsis seeds, alfalfa leaves, and delays dark-induced leaf senescence.

    Science.gov (United States)

    Jiang, Jishan; Jia, Huili; Feng, Guangyan; Wang, Zan; Li, Jun; Gao, Hongwen; Wang, Xuemin

    2016-08-01

    Alfalfa (Medicago sativa L.) is a major forage legume for livestock and a target for improving their dietary quality. Vitamin E is an essential vitamin that animals must obtain from their diet for proper growth and development. γ-tocopherol methyltransferase (γ-TMT), which catalyzes the conversion of δ- and γ-tocopherols (or tocotrienols) to β- and α-tocopherols (or tocotrienols), respectively, is the final enzyme involved in the vitamin E biosynthetic pathway. The overexpression of M. sativa L.'s γ-TMT (MsTMT) increased the α-tocopherol content 10-15 fold above that of wild type Arabidopsis seeds without altering the total content of vitamin E. Additionally, in response to osmotic stress, the biomass and the expression levels of several osmotic marker genes were significantly higher in the transgenic lines compared with wild type. Overexpression of MsTMT in alfalfa led to a modest, albeit significant, increase in α-tocopherol in leaves and was also responsible for a delayed leaf senescence phenotype. Additionally, the crude protein content was increased, while the acid and neutral detergent fiber contents were unchanged in these transgenic lines. Thus, increased α-tocopherol content occurred in transgenic alfalfa without compromising the nutritional qualities. The targeted metabolic engineering of vitamin E biosynthesis through MsTMT overexpression provides a promising approach to improve the α-tocopherol content of forage crops.

  1. Expression of an alfalfa (Medicago sativa L.) ethylene response factor gene MsERF8 in tobacco plants enhances resistance to salinity.

    Science.gov (United States)

    Chen, Tingting; Yang, Qingchuan; Gruber, Margaret; Kang, Junmei; Sun, Yan; Ding, Wang; Zhang, Tiejun; Zhang, Xinquan

    2012-05-01

    Ethylene response factors (ERF) play crucial roles in plant development and response to stresses. Here, a novel cDNA fragment (MsERF8) encoding an ERF protein with an AP2 domain was isolated and characterized from alfalfa. The MsERF8 cDNA has an open reading frame of 603 bp and encodes a nuclear protein of 201 amino acids. Q-RT-PCR analysis revealed that MsERF8 was strongly enriched in roots and leaves compared with stems, flower buds and flowers of mature alfalfa plants. Bioinformatic analysis of the MsERF8 promoter indicated a number of elements associated with stress-related responses, and MsERF8 transcripts in alfalfa seedlings were induced by NaCl, PEG6000, Al(2)(SO(4))(3) and five different hormones. Expression of MsERF8 in transgenic tobacco plants resulted in higher tolerance to salinity than with non-transgenic plants. This data shows that MsERF8 is a gene which prevents or alleviates salinity damage and has strong potential to impart salt tolerance to other crop plants.

  2. El cultivo de alfalfa utilizando agua de perforación, agua residual urbana y precipitaciones The cultivation of alfalfa using artesian well water, urban wastewater and rainfall

    Directory of Open Access Journals (Sweden)

    José O. Plevich

    2012-12-01

    Full Text Available El objetivo de este trabajo fue analizar la producción de biomasa aérea, la eficiencia del uso de agua y el valor nutritivo de alfalfa cultivada aplicando riego con agua residual urbana, agua de perforación y un testigo (precipitaciones, en el Campus de la Universidad Nacional de Rio Cuarto (Córdoba-Argentina. Los tratamientos de riego tuvieron un efecto positivo sobre la producción de biomasa aérea obteniéndose un 24% más de producción de biomasa que en la situación de secano (precipitaciones. Entre los tratamientos con riego también se manifestaron diferencias estadísticamente significativas. La alfalfa regada con aguas residuales urbana supero en un 19% a la cultivada con agua de perforación. La eficiencia del uso del agua por parte del cultivo se incrementó cuando el riego se realizó con aguas residuales urbanas. Además, se determinó que la alfalfa regada con agua residual, supera los valores nutritivos del cultivo que crece en condiciones sin riego; encontrándose 39% más de proteínas, 14% más de digestibilidad e igual porcentaje de energía metabólica. Se puede concluir que el agua residual urbana representa otra posible fuente de agua, factible de ingresar al suelo y estar a disposición para el cultivo de alfalfa.The objective of this study was to analyse the production of alfalfa biomass, the efficiency of water use and the nutritional value of forage, inside an irrigation structure of an experimental facility that uses treated urban wastewater, artesian well water and rainfall, at the campus of the Universidad National de Rio Cuarto (Córdoba-Argentina. The irrigation treatment had a positive effect on the production of biomass of alfalfa. On an average 24% increase in production was observed when compared to dry-land farming. Regarding the quality of the irrigation water, statistical differences in production were observed, the irrigation with urban wastewater produced 19% more than that of well water. The

  3. Germinación y crecimiento de alfalfa bajo condiciones salinas Germination and growth of alfalfa under saline conditions

    Directory of Open Access Journals (Sweden)

    Sara Lucía González-Romero

    2011-02-01

    Full Text Available En México, el cultivo de la alfalfa (Medicago sativa L. se emplea para la alimentación del ganado bovino, éste cuenta con investigaciones de respuestas a estrés salino en la etapa de germinación; sin embargo, sólo se han usado diferentes niveles de NaCl o de manitol y polietilenglicol; por lo anterior, los objetivos de esta investigación fue estudiar la respuesta a la salinidad, que se presenta en zonas áridas y semiáridas de México de la alfalfa en la etapa de germinación y evaluar su efecto sobre el crecimiento de la radícula y la parte aérea. Para cumplir lo anterior, en el laboratorio de salinidad del Colegio de Postgraduados en Ciencias Agrícolas durante el 2009, las semillas se sometieron a pruebas de germinación en un diseño factorial de 11 soluciones salinas a ocho dosis (0, 2, 4, 8, 11.7, 15, 19 y 28 dS m-1 y tres repeticiones. Se observó que las sales NaHCO3 y sulfático-sódica influyeron más sobre la germinación; y las sales CaCl2 2H2O y sulfática registraron el mayor porcentaje de germinación; para el crecimiento de radícula la sal que más afectó fue el NaHCO3 y la salinidad sulfática; las sales CaCl2 2H2O y sulfático-clorhídrica permitieron mayor desarrollo. El crecimiento de la parte aérea fue más afectado en comparación al desarrollo de la radícula. La mezcla de sales favoreció el porcentaje de germinación, el crecimiento de radícula y parte aérea.In Mexico, alfalfa (Medicago sativa L. is used for feeding cattle and has been studied for responses to salt stress in the germination phase; however, only NaCl or mannitol and polyethylene glycols have been used in different levels. Due to this, the aims of this research were to study the response of alfalfa in the germination stage to the salinity of the arid and semiarid areas in Mexico, and to evaluate its effect on the growth of the radicle and aerial section. In order to achieve this, in the salinity lab of the Colegio de Postgraduados en

  4. Varieties of capitalism in crisis?

    DEFF Research Database (Denmark)

    Bruff, Ian; Horn, Laura

    2012-01-01

    In this introduction to the special issue, we provide a brief overview of the two distinct but connected meanings of the phrase ‘varieties of capitalism in crisis’: (1) the crisis of empirical political economies under conditions of capitalist diversity; and (2) the crisis of the institutionalist...

  5. Classification of Irregular Varieties : Minimal Models and Abelian Varieties

    CERN Document Server

    Catanese, Fabrizio; Ciliberto, Ciro

    1992-01-01

    M. Andreatta,E.Ballico,J.Wisniewski: Projective manifolds containing large linear subspaces; - F.Bardelli: Algebraic cohomology classes on some specialthreefolds; - Ch.Birkenhake,H.Lange: Norm-endomorphisms of abelian subvarieties; - C.Ciliberto,G.van der Geer: On the jacobian of ahyperplane section of a surface; - C.Ciliberto,H.Harris,M.Teixidor i Bigas: On the endomorphisms of Jac (W1d(C)) when p=1 and C has general moduli; - B. van Geemen: Projective models of Picard modular varieties; - J.Kollar,Y.Miyaoka,S.Mori: Rational curves on Fano varieties; - R. Salvati Manni: Modular forms of the fourth degree; A. Vistoli: Equivariant Grothendieck groups and equivariant Chow groups; - Trento examples; Open problems

  6. Fatty acid profiling of the seed oils of some varieties of field peas (Pisum sativum) by RP-LC/ESI-MS/MS: towards the development of an oilseed pea.

    Science.gov (United States)

    Villalobos Solis, Manuel Ivan; Patel, Anil; Orsat, Valérie; Singh, Jaswinder; Lefsrud, Mark

    2013-08-15

    Reversed-phase liquid chromatography coupled to negative-ion electrospray tandem mass spectrometry (RP-LC/ESI-MS/MS) was used to study the fatty acid profile from the oil of harvested field pea (Pisum sativum) varieties as part of a research project to develop this legume as a commercial oilseed for Canada. The seed oils from pea samples contained palmitic and stearic acids as major saturated fatty acids. Oleic, linoleic and linolenic acids were the major unsaturated fatty acids found. Small percentages of other long chain fatty acids were also detected. This profile suggests that the species of field pea investigated might have the potential to be used as raw materials to develop a future new oilseed crop for the food industry. Fatty acid extracts did not require further manipulation before final analysis by RP-LC/ESI-MS/MS, indicating the utility and relative simplicity of this technique for future screening studies.

  7. Transcript profiling of two alfalfa genotypes with contrasting cell wall composition in stems using a cross-species platform: optimizing analysis by masking biased probes

    Directory of Open Access Journals (Sweden)

    Jung Hans-Joachim G

    2010-05-01

    Full Text Available Abstract Background The GeneChip® Medicago Genome Array, developed for Medicago truncatula, is a suitable platform for transcript profiling in tetraploid alfalfa [Medicago sativa (L. subsp. sativa]. However, previous research involving cross-species hybridization (CSH has shown that sequence variation between two species can bias transcript profiling by decreasing sensitivity (number of expressed genes detected and the accuracy of measuring fold-differences in gene expression. Results Transcript profiling using the Medicago GeneChip® was conducted with elongating stem (ES and post-elongation stem (PES internodes from alfalfa genotypes 252 and 1283 that differ in stem cell wall concentrations of cellulose and lignin. A protocol was developed that masked probes targeting inter-species variable (ISV regions of alfalfa transcripts. A probe signal intensity threshold was selected that optimized both sensitivity and accuracy. After masking for both ISV regions and previously identified single-feature polymorphisms (SFPs, the number of differentially expressed genes between the two genotypes in both ES and PES internodes was approximately 2-fold greater than the number detected prior to masking. Regulatory genes, including transcription factor and receptor kinase genes that may play a role in development of secondary xylem, were significantly over-represented among genes up-regulated in 252 PES internodes compared to 1283 PES internodes. Several cell wall-related genes were also up-regulated in genotype 252 PES internodes. Real-time quantitative RT-PCR of differentially expressed regulatory and cell wall-related genes demonstrated increased sensitivity and accuracy after masking for both ISV regions and SFPs. Over 1,000 genes that were differentially expressed in ES and PES internodes of genotypes 252 and 1283 were mapped onto putative orthologous loci on M. truncatula chromosomes. Clustering simulation analysis of the differentially expressed genes

  8. Production Technical Rule of Alfalfa under Sprinkler Irrigation on the Thin and Arenaceous Soil at Ili River Basin in Xinjiang%新疆伊犁河流域砂质薄土层苜蓿喷灌生产技术规程

    Institute of Scientific and Technical Information of China (English)

    丁峰; 秦巧; 蒲胜海; 冯广平; 杨培林; 段生莲; 马雪琴

    2012-01-01

    为了规范新疆伊犁河流域砂质薄土层苜蓿的生产,针对伊犁河流域水土开发区域面临砂质薄土层的灌溉问题和苜蓿生产中存在的问题,依据国家及行业标准,对新疆伊犁河流域砂质薄土层新垦耕地的喷灌苜蓿种植的灌溉生产管理等提出了具体要求.针对新疆伊犁河流域砂质薄土层新垦耕地特殊的地理气候条件,对其苜蓿生产中的品种选择、培肥地力、平整土地、灌溉管理和配套栽培措施等进行了具体规定.该生产技术规程的实施对新疆伊犁河流域砂质薄土层新垦耕地苜蓿喷灌生产具有重要的现实指导意义,将显著提高饲草产量,增加农民收入,促进新疆伊犁州畜牧业的可持续发展和农村经济的快速发展.%In order to regulate the production of alfalfa on thin and arenaceous soil which was newly cultivated at Ili River basin in Xinjiang, a technical rule was presented based on national and industry standard, and the problems in irrigation question and alfalfa production on thin and arenaceous soil at Hi River basin. The technical rule was against the specific geological and climate conditions of the newly cultivated land on thin and arenaceous soil at Ili River basin in Xinjiang. It concluded variety selection, fertilization ability, land levelling, irrigation management and relevant cultivation measures. This production technical rule could be a reference to alfalfa production with sprinkler irrigation on the thin and arenaceous soil at Hi River basin in Xinjiang. With its implementation the yield of forage grass and fanner's income might be largely increased and it might promote sustainable development of animal husbandry and rural economy in Xinjiang.

  9. Vector bundles on toric varieties

    CERN Document Server

    Gharib, Saman

    2011-01-01

    Following Sam Payne's work, we study the existence problem of nontrivial vector bundles on toric varieties. The first result we prove is that every complete fan admits a nontrivial conewise linear multivalued function. Such functions could potentially be the Chern classes of toric vector bundles. Then we use the results of Corti\\~nas, Haesemeyer, Walker and Weibel to show that the (non-equivariant) Grothendieck group of the toric 3-fold studied by Payne is large, so the variety has a nontrivial vector bundle. Using the same computation, we show that every toric 3-fold X either has a nontrivial line bundle, or there is a finite surjective toric morphism from Y to X, such that Y has a large Grothendieck group.

  10. Positroid Varieties: Juggling and Geometry

    CERN Document Server

    Knutson, Allen; Speyer, David

    2011-01-01

    While the intersection of the Grassmannian Bruhat decompositions for all coordinate flags is an intractable mess, the intersection of only the cyclic shifts of one Bruhat decomposition turns out to have many of the good properties of the Bruhat and Richardson decompositions. This decomposition coincides with the projection of the Richardson stratification of the flag manifold, studied by Lusztig, Rietsch, Brown-Goodearl-Yakimov and the present authors. However, its cyclic-invariance is hidden in this description. Postnikov gave many cyclic-invariant ways to index the strata, and we give a new one, by a subset of the affine Weyl group we call bounded juggling patterns. We call the strata positroid varieties. Applying results from the authors' previous work, we show that positroid varieties are normal, Cohen-Macaulay, have rational singularities, and are defined as schemes by the vanishing of Plucker coordinates. We prove that their associated cohomology classes are represented by affine Stanley functions. This...

  11. Schubert varieties and degeneracy loci

    CERN Document Server

    Fulton, William

    1998-01-01

    Schubert varieties and degeneracy loci have a long history in mathematics, starting from questions about loci of matrices with given ranks. These notes, from a summer school in Thurnau, aim to give an introduction to these topics, and to describe recent progress on these problems. There are interesting interactions with the algebra of symmetric functions and combinatorics, as well as the geometry of flag manifolds and intersection theory and algebraic geometry.

  12. Real Multiplication on Jacobian Varieties

    CERN Document Server

    Kucharczyk, Robert A

    2012-01-01

    This is a slightly revised version of the author's 2010 diploma thesis. It is concerned with the interplay between real multiplication on Jacobian varieties, as the title suggests, and complex geodesics in the moduli space of curves. Large parts are expository and may hopefully serve as a very incomplete introduction to Teichmueller disks and curves, the moduli space of abelian differentials with its SL2(R)-operation and variations of Hodge structure.

  13. Operator algebras for analytic varieties

    CERN Document Server

    Davidson, Kenneth R; Shalit, Orr Moshe

    2012-01-01

    We study the isomorphism problem for the multiplier algebras of irreducible complete Pick kernels. These are precisely the restrictions $\\cM_V$ of the multiplier algebra $\\cM$ of Drury-Arveson space to a holomorphic subvariety $V$ of the unit ball. The related algebras of continuous multipliers are also considered. We find that $\\cM_V$ is completely isometrically isomorphic to $\\cM_W$ if and only if $W$ is the image of $V$ under a biholomorphic automorphism of the ball. A similar condition characterizes when there exists a unital completely contractive homomorphism from $\\cM_V$ to $\\cM_W$. If one of the varieties is a homogeneous algebraic variety, then isometric isomorphism is shown to imply completely isometric isomorphism of the algebras. The problem of characterizing when two such algebras are (algebraically) isomorphic is also studied. It is shown that if there is an isomorphism between $\\cM_V$ and $\\cM_W$, then there is a biholomorphism (with multiplier coordinates) between the varieties. We present a n...

  14. Screening of Cd tolerant genotypes and isolation of metallothionein genes in alfalfa (Medicago sativa L.)

    Energy Technology Data Exchange (ETDEWEB)

    Wang Xiaojuan, E-mail: xiaojuanwang@lzu.edu.cn [School of Pastoral Agriculture Science and Technology, Lanzhou University, P.O. Box 61, Lanzhou 730020 (China); Song, Yu [School of Pastoral Agriculture Science and Technology, Lanzhou University, P.O. Box 61, Lanzhou 730020 (China); Environment Management College of China, Qinhuangdao 066004 (China); Ma Yanhua [Hebei Normal University of Science and Technology, Qinhuangdao 066004 (China); Zhuo Renying [Key Lab of Tree Genomics, Research Institute of Subtropical of Forest, Chinese Academy of Forest, Fuyang 311400 (China); Jin Liang [School of Pastoral Agriculture Science and Technology, Lanzhou University, P.O. Box 61, Lanzhou 730020 (China)

    2011-12-15

    In order to evaluate Cd tolerance in wide-ranging sources of alfalfa (Medicago sativa) and to identify Cd tolerant genotypes which may potentially be useful for restoring Cd-contaminated environments, thirty-six accessions of alfalfa were screened under hydroponic culture. Our results showed that the relative root growth rate varied from 0.48 to 1.0, which indicated that different alfalfa accessions had various responses to Cd stress. The candidate fragments derived from differentially expressed metallothionein (MT) genes were cloned from leaves of two Cd tolerant genotypes, YE and LZ. DNA sequence and the deduced protein sequence showed that MsMT2a and MsMT2b had high similarity to those in leguminous plants. DDRT-PCR analysis showed that MsMT2a expressed in both YE and LZ plants under control and Cd stress treatment, but MsMT2b only expressed under Cd stress treatment. This suggested that MsMT2a was universally expressed in leaves of alfalfa but expression of MsMT2b was Cadmium (Cd) inducible. - Highlights: > Evaluate Cd tolerance in wide sources of alfalfa accessions. > Identify Cd-hyperaccumulators potentially useful for restoring Cd-contaminated environments. > Cloned differentially expressed metallothionein (MT) genes. > Characteristics and deduced protein sequence of MsMT2a and MsMT2b were analyzed. > MsMT2a might be a universally gene of alfalfa but MsMT2b might be an inductive gene. - Two Cd tolerant alfalfa genotypes were screened and their metallothionein genes were cloned which showed that MsMT2a was universally expressed but MsMT2b was Cd inducible expression.

  15. Contribution of exopeptidases to formation of nonprotein nitrogen during ensiling of alfalfa.

    Science.gov (United States)

    Tao, L; Zhou, H; Guo, X S; Long, R J; Zhu, Y; Cheng, W

    2011-08-01

    The experiment was conducted to investigate the exopeptidase classes in alfalfa (Medicago sativa L.) leaves, and to determine their contribution to the formation of nonprotein nitrogen (NPN) components during ensiling. Six classes of inhibitors that included bestatin (aminopeptidase inhibitor), potato carboxypeptidase inhibitor (PCI, carboxypeptidase inhibitor), 1,10-phenanthroline (dipeptidase inhibitor), diprotin A (dipeptidyl-peptidase inhibitor), butabindide (tripeptidyl-peptidase inhibitor), and dipeptide Phe-Arg (peptidyl-dipeptidase inhibitor) were used. To determine the contribution of each exopeptidase to the formation of NPN products, aqueous extracts of fresh alfalfa were fermented to imitate the proteolytic process of ensiled alfalfa and to ensure that each class of exopeptidase inhibitor would have immediate contact with the proteases in the alfalfa extract. Five classes of exopeptidases; namely, aminopeptidase, carboxypeptidase, dipeptidase, dipeptidyl-peptidase, and tripeptidyl-peptidase, were shown to be present in alfalfa leaves, each playing a different role in alfalfa protein degradation. Aminopeptidase, carboxypeptidase, and dipeptidase were the main exopeptidases contributing to the formation of NH(3)-N. Among the 5 exopeptidases, tripeptidyl-peptidase appeared to be the principal exopeptidase in hydrolyzing forage protein into peptides, whereas carboxypeptidase and dipeptidase appeared to be more important in contributing to the formation of amino acid-N. Dipeptidyl-peptidase and tripeptidyl-peptidase did not play a role in the formation of NH(3)-N or amino acid-N. Dipeptidase, carboxypeptidase, and tripeptidyl-peptidase were the principal exopeptidases for hydrolyzing forage protein into NPN during ensilage, and treatment with a mixture of the 5 inhibitors reduced the total NPN concentration in the fermented alfalfa extract to about 45% of that in the control after 21 d of fermentation.

  16. Transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode Meloidogyne incognita.

    Science.gov (United States)

    Postnikova, Olga A; Hult, Maria; Shao, Jonathan; Skantar, Andrea; Nemchinov, Lev G

    2015-01-01

    Nematodes are one of the major limiting factors in alfalfa production. Root-knot nematodes (RKN, Meloidogyne spp.) are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. As of today, no studies have been published on global gene expression profiling in alfalfa infected with RKN or any other plant parasitic nematode. Very little information is available about molecular mechanisms that contribute to pathogenesis and defense responses in alfalfa against these pests and specifically against RKN. In this work, we performed root transcriptome analysis of resistant (cv. Moapa 69) and susceptible (cv. Lahontan) alfalfa cultivars infected with RKN Meloidogyne incognita, widespread root-knot nematode species and a major pest worldwide. A total of 1,701,622,580 pair-end reads were generated on an Illumina Hi-Seq 2000 platform from the roots of both cultivars and assembled into 45,595 and 47,590 transcripts in cvs Moapa 69 and Lahontan, respectively. Bioinformatic analysis revealed a number of common and unique genes that were differentially expressed in susceptible and resistant lines as a result of nematode infection. Although the susceptible cultivar showed a more pronounced defense response to the infection, feeding sites were successfully established in its roots. Characteristically, basal gene expression levels under normal conditions differed between the two cultivars as well, which may confer advantage to one of the genotypes toward resistance to nematodes. Differentially expressed genes were subsequently assigned to known Gene Ontology categories to predict their functional roles and associated biological processes. Real-time PCR validated expression changes in genes arbitrarily selected for experimental confirmation. Candidate genes that contribute to protection against M. incognita in alfalfa were proposed and alfalfa-nematode interactions with respect to resistance

  17. Analysis of the alfalfa root transcriptome in response to salinity stress.

    Science.gov (United States)

    Postnikova, Olga A; Shao, Jonathan; Nemchinov, Lev G

    2013-07-01

    Salinity is one of the major abiotic factors affecting alfalfa productivity. Identifying genes that control this complex trait will provide critical insights for alfalfa breeding programs. To date, no studies have been published on a deep sequencing-based profiling of the alfalfa transcriptome in response to salinity stress. Observations gathered through research on reference genomes may not always be applicable to alfalfa. In this work, Illumina RNA-sequencing was performed in two alfalfa genotypes contrasting in salt tolerance, in order to estimate a broad spectrum of genes affected by salt stress. A total of 367,619,586 short reads were generated from cDNA libraries originated from roots of both lines. More than 60,000 tentative consensus sequences (TCs) were obtained and, among them, 74.5% had a significant similarity to proteins in the NCBI database. Mining of simple sequence repeats (SSRs) from all TCs revealed 6,496 SSRs belonging to 3,183 annotated unigenes. Bioinformatics analysis showed that the expression of 1,165 genes, including 86 transcription factors (TFs), was significantly altered under salt stress. About 40% of differentially expressed genes were assigned to known gene ontology (GO) categories using Arabidopsis GO. A random check of differentially expressed genes by quantitative real-time PCR confirmed the bioinformatic analysis of the RNA-seq data. A number of salt-responsive genes in both tested genotypes were identified and assigned to functional classes, and gene candidates with roles in the adaptation to salinity were proposed. Alfalfa-specific data on salt-responsive genes obtained in this work will be useful in understanding the molecular mechanisms of salinity tolerance in alfalfa.

  18. Transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode Meloidogyne incognita.

    Directory of Open Access Journals (Sweden)

    Olga A Postnikova

    Full Text Available Nematodes are one of the major limiting factors in alfalfa production. Root-knot nematodes (RKN, Meloidogyne spp. are widely distributed and economically important sedentary endoparasites of agricultural crops and they may inflict significant damage to alfalfa fields. As of today, no studies have been published on global gene expression profiling in alfalfa infected with RKN or any other plant parasitic nematode. Very little information is available about molecular mechanisms that contribute to pathogenesis and defense responses in alfalfa against these pests and specifically against RKN. In this work, we performed root transcriptome analysis of resistant (cv. Moapa 69 and susceptible (cv. Lahontan alfalfa cultivars infected with RKN Meloidogyne incognita, widespread root-knot nematode species and a major pest worldwide. A total of 1,701,622,580 pair-end reads were generated on an Illumina Hi-Seq 2000 platform from the roots of both cultivars and assembled into 45,595 and 47,590 transcripts in cvs Moapa 69 and Lahontan, respectively. Bioinformatic analysis revealed a number of common and unique genes that were differentially expressed in susceptible and resistant lines as a result of nematode infection. Although the susceptible cultivar showed a more pronounced defense response to the infection, feeding sites were successfully established in its roots. Characteristically, basal gene expression levels under normal conditions differed between the two cultivars as well, which may confer advantage to one of the genotypes toward resistance to nematodes. Differentially expressed genes were subsequently assigned to known Gene Ontology categories to predict their functional roles and associated biological processes. Real-time PCR validated expression changes in genes arbitrarily selected for experimental confirmation. Candidate genes that contribute to protection against M. incognita in alfalfa were proposed and alfalfa-nematode interactions with

  19. (Almost) Dark HI Sources in the ALFALFA Survey: The Intriguing Case of HI1232+20

    Science.gov (United States)

    Janowiecki, Steven; Leisman, Lukas; Józsa, Gyula; Salzer, John J.; Haynes, Martha P.; Giovanelli, Riccardo; Rhode, Katherine L.; Cannon, John M.; Adams, Elizabeth A. K.; Janesh, William F.

    2015-03-01

    We report the discovery and follow-up observations of a system of three objects identified by the ALFALFA extragalactic HI survey, cataloged as (almost) dark extragalactic sources, i.e., extragalactic HI detections with no discernible counterpart in publicly available, wide-field, imaging surveys. We have obtained deep optical imaging with WIYN pODI and HI synthesis maps with WSRT of the HI1232+20 system. The source with the highest HI flux has a newly discovered ultra-low surface brightness (LSB) optical counterpart associated with it, while the other two sources have no detected optical counterparts in our images. Our optical observations show that the detected LSB optical counterpart has a peak surface brightness of ˜26.4 mag arcsec-2 in g‧, which is exceptionally faint. This source (AGC 229385) has the largest accurately measured HI mass-to-light ratio of an isolated object: {{M}HI}/{{L}{{g\\prime }}} = 46 {{M}⊙ }/{{L}⊙ }, and has an HI mass of 7.2 × {{10}8} {{M}⊙ }. The other two HI sources (with HI masses 2.0 × 108 and 1.2 × {{10}8} {{M}⊙ }) without optical counterparts have upper limit surface brightnesses of 27.9 and 27.8 mag arcsec-2 in g‧, and lower limits on their gas mass-to-light ratio of {{M}HI}/{{L}{{g\\prime }}} > 57 and > 31 {{M}⊙ }/{{L}⊙ }. This system lies relatively close in projection to the Virgo Cluster, but velocity flow models indicate that it is located at 25 Mpc, substantially beyond Virgo. The system appears to be quite isolated, with no known object closer than ˜500 kpc. These HI sources may represent both sides of the threshold between “dark” star-less galaxies and galaxies with stellar populations. We discuss a variety of possible formation scenarios for the HI1232+20 system.

  20. Effects of additive application upon ad libitum intake, in vivo digestibility and nitrogen balance of alfalfa haylage

    Directory of Open Access Journals (Sweden)

    Mladen Knežević

    2009-09-01

    Full Text Available The research objective was to determine the effect of the additive Sill-All application on ad libitum intake, in vivo digestibility and nitrogen balance of alfalfa haylage. About 40 % alfalfa plants was ensiled at the phonological flowering stage into plastic foil-wrapped bales without or with additive (2 L t-1 plant mass. No statistically significant differences were found between alfalfa ensiled without additive and alfalfa ensiled with additive for the content of dry matter (DM (632 g and 631 g DM kg-1 fresh sample, respectively. Alfalfa ensiled without additive contained 921 g organic matter (OM kg-1 DM, which was significantly higher (P<0.001 compared to alfalfa ensiled with additive (902 g OM kg-1 DM. Alfalfa ensiled without additive contained 141 g crude proteins (CP kg-1 DM, which was significantly higher (P<0.001 compared to alfalfa ensiled with additive (139 g CP kg-1 DM. Alfalfa ensiled with additive contained significantly less acid detergent fibers (ADF (P<0.001 compared to alfalfa ensiled without additive (445 g kg-1 DM and 456 g kg-1 DM, respectively and had a lower pH value (P<0.001 (5.29 and 5.56, respectively. No statistically significant differences were found between the studied feeding treatments for ad libitum intake of fresh ration and DM ration, for the measured parameters of digestibility and N balance. It was concluded that addition of the additive to alfalfa haylage led to significant changes in chemical composition; however, changes in chemical composition had no impact on measured biological parameters (ad libitum intake, in vivo digestibility and nitrogen balance.

  1. Relationship of red and photographic infrared spectral radiances to alfalfa biomass, forage water content, percentage canopy cover, and severity of drought stress

    Science.gov (United States)

    Tucker, C. J.; Elgin, J. H., Jr.; Mcmurtrey, J. E., III

    1979-01-01

    Red and photographic infrared spectral data were collected using a handheld radiometer for two cuttings of alfalfa. Significant linear and non-linear correlation coefficients were found between the spectral variables and plant height, biomass, forage water content, and estimated canopy cover for the earlier alfalfa cutting. The alfalfa of later cutting experienced a period of severe drought stress which limited growth. The spectral variables were found to be highly correlated with the estimated drought scores for this alfalfa cutting.

  2. Combining -Omics to Unravel the Impact of Copper Nutrition on Alfalfa (Medicago sativa) Stem Metabolism.

    Science.gov (United States)

    Printz, Bruno; Guerriero, Gea; Sergeant, Kjell; Audinot, Jean-Nicolas; Guignard, Cédric; Renaut, Jenny; Lutts, Stanley; Hausman, Jean-Francois

    2016-02-01

    Copper can be found in the environment at concentrations ranging from a shortage up to the threshold of toxicity for plants, with optimal growth conditions situated in between. The plant stem plays a central role in transferring and distributing minerals, water and other solutes throughout the plant. In this study, alfalfa is exposed to different levels of copper availability, from deficiency to slight excess, and the impact on the metabolism of the stem is assessed by a non-targeted proteomics study and by the expression analysis of key genes controlling plant stem development. Under copper deficiency, the plant stem accumulates specific copper chaperones, the expression of genes involved in stem development is decreased and the concentrations of zinc and molybdenum are increased in comparison with the optimum copper level. At the optimal copper level, the expression of cell wall-related genes increases and proteins playing a role in cell wall deposition and in methionine metabolism accumulate, whereas copper excess imposes a reduction in the concentration of iron in the stem and a reduced abundance of ferritins. Secondary ion mass spectrometry (SIMS) analysis suggests a role for the apoplasm as a copper storage site in the case of copper toxicity.

  3. Functional characterization of a gibberellin receptor and its application in alfalfa biomass improvement

    Science.gov (United States)

    Wang, Xuemin; Li, Jun; Ban, Liping; Wu, Yudi; Wu, Xinming; Wang, Yunqi; Wen, Hongyu; Chapurin, Vladimir; Dzyubenko, Nikolay; Li, Zhiyong; Wang, Zan; Gao, Hongwen

    2017-01-01

    Bioactive gibberellins (GAs) are essential phytohormones involved in the regulation of many aspects of plant development. GA receptors are crucial in GA signal transduction in plants. The GA receptor GoGID1 promotes plant elongation and improves biomass production when ectopically expressed in tobacco. Here, we discovered that GoGID1 can interact with the DELLA proteins of Arabidopsis in the presence of gibberellic acid. GoGID1 partially or completely functionally rescued the phenotypes of the Arabidopsis double-mutants atgid1a/atgid1c and atgid1a/atgid1b. The overexpression of GoGID1 led to increases in plant height and biomass production in transgenic Arabidopsis plants. The GoGID1 gene enhanced GA sensitivity of the transgenic plants. More importantly, transgenic alfalfa plants overexpressing GoGID1 exhibited increased growth rates, heights and biomass and produced larger leaves when compared with the control plants. Thus, GoGID1 functions as a GA receptor, playing multiple roles in plant growth and development. The GoGID1 gene has the potential to be used in the genetic engineering of forage crops for biomass improvement. PMID:28128230

  4. Weil classes on abelian varieties

    CERN Document Server

    Moonen, B J J; Zarhin, Yu. G.

    1996-01-01

    Consider a complex abelian variety X on which a field F acts. Generalizing a construction of A. Weil, one associates to this a subspace W_F of the cohomology of X, which we call the space of Weil classes w.r.t. F. The purpose of this paper is to answer the following two questions: Q1: under what conditions on F does the space W_F contain, or even consist of, Hodge classes?, Q2: if W_F contains Hodge classes, under what conditions on F are these exceptional? In case X is defined over a number field, we also answer the analogous questions for Tate classes.

  5. Homology theory on algebraic varieties

    CERN Document Server

    Wallace, Andrew H

    2014-01-01

    Concise and authoritative, this monograph is geared toward advanced undergraduate and graduate students. The main theorems whose proofs are given here were first formulated by Lefschetz and have since turned out to be of fundamental importance in the topological aspects of algebraic geometry. The proofs are fairly elaborate and involve a considerable amount of detail; therefore, some appear in separate chapters that include geometrical descriptions and diagrams.The treatment begins with a brief introduction and considerations of linear sections of an algebraic variety as well as singular and h

  6. Varieties of Secularism in Asia

    DEFF Research Database (Denmark)

    Varieties of Secularism is an ethnographically rich, theoretically well-informed, and intellectually coherent volume which builds off the work of Talal Asad, Charles Taylor, and others who have engaged the issue of secularism(s) and in socio-political life. The volume seeks to examine theories of...... phenomena; between the new visibilities and silences of magic, ancestors, and religion in democratic politics, this book seeks to outline the particular formations of secularism that have become possible in Asia from China to Indonesia and from Bahrain to Timor-Leste....

  7. Uniruledness of orthogonal modular varieties

    CERN Document Server

    Gritsenko, Valery

    2012-01-01

    A strongly reflective modular form with respect to an orthogonal group of signature (2,n) determines a Lorentzian Kac--Moody algebra. We find a new geometric application of such modular forms: we prove that if the weight is larger than n then the corresponding modular variety is uniruled. We also construct new reflective modular forms and thus provide new examples of uniruled moduli spaces of lattice polarised K3 surfaces. Finally we prove that the moduli space of Kummer surfaces associated to (1,21)-polarised abelian surfaces is uniruled.

  8. Schubert Unions in Grassmann Varieties

    DEFF Research Database (Denmark)

    Hansen, Johan Peder; Johnsen, T.; Ranestad, K.

    We study subsets of Grassmann varieties G(l,m) over a field F, such that these subsets are unions of Schubert cycles, with respect to a fixed flag. We study the linear spans of, and in case of positive characteristic, the number of points on such unions over finite fields. Moreover we study...... a geometric duality of such unions, and give a combinatorial interpretation of this duality. We discuss the maximum number of points over a finite field for the Schubert unions of a given spanning dimension, and we give some applications to coding theory. We define Schubert union codes, and study...

  9. Cryopreservation of Bituminaria bituminosa varieties and hybrids

    OpenAIRE

    Gisbert Domenech, Maria Carmen; Dabauza, Mercedes; Correal, Enrique; Swennen, Rony; Panis, Bart

    2015-01-01

    [EN] Bituminaria bituminosa (L.) C.H. Stirton is a drought tolerant, perennial legume pasture species and a source of pharmaceutical compounds. Bituminaria breeding programs aim to develop and conserve hybrids with desirable traits such as high forage quality, tolerance to biotic or abiotic stresses, and high contents of furanocoumarins. In this work we present a cryopreservation study of different B. bituminosa accessions: two varieties and eight intervarietal hybrids resulting f...

  10. Toric varieties, monoid schemes and $cdh$ descent

    CERN Document Server

    Cortiñas, Guillermo; Walker, Mark E; Weibel, Charles A

    2011-01-01

    We give conditions for the Mayer-Vietoris property to hold for the algebraic K-theory of blow-up squares of toric varieties in any characteristic, using the theory of monoid schemes. These conditions are used to relate algebraic K-theory to topological cyclic homology in characteristic p. To achieve our goals, we develop for monoid schemes many notions from classical algebraic geometry, such as separated and proper maps.

  11. Metric Diophantine approximation on homogeneous varieties

    CERN Document Server

    Ghosh, Anish; Nevo, Amos

    2012-01-01

    We develop the metric theory of Diophantine approximation on homogeneous varieties of semisimple algebraic groups and prove results analogous to the classical Khinchin and Jarnik theorems. In full generality our results establish simultaneous Diophantine approximation with respect to several completions, and Diophantine approximation over general number fields using S-algebraic integers. In several important examples, the metric results we obtain are optimal. The proof uses quantitative equidistribution properties of suitable averaging operators, which are derived from spectral bounds in automorphic representations.

  12. Enteric methane emission, diet digestibility, and nitrogen excretion from beef heifers fed sainfoin or alfalfa.

    Science.gov (United States)

    Chung, Y-H; Mc Geough, E J; Acharya, S; McAllister, T A; McGinn, S M; Harstad, O M; Beauchemin, K A

    2013-10-01

    Effects of plant-bound condensed tannin (CT)-containing sainfoin vs. CT-free alfalfa (or low-CT alfalfa-sainfoin mixture), plant stage of maturity, and their interaction on enteric methane (CH4) emissions, diet digestibility, and N excretion were studied, using 8 ruminally cannulated beef heifers in 2 sequential short-term experiments (Exp. 1 and 2). In Exp. 1, first growth legumes were harvested daily and offered fresh to heifers. Heifers were assigned to 100% sainfoin or 80% alfalfa:20% sainfoin (as-fed basis). Responses were measured at early (late vegetative to early bud; stage 2 to 3) and late (early flower; stage 5) stage of maturity. In Exp. 2, the same legumes were harvested from second growth (late bud; stage 4) and offered to heifers as hay; 100% sainfoin or 100% alfalfa. In both experiments, heifers were fed once daily at 1× maintenance. When fed as fresh forage (Exp. 1), sainfoin, compared with the alfalfa-sainfoin blend, had greater digestibility of OM (74.7 vs. 70.9%; P = 0.02), yet tended to have lower CP digestibility (73.2 vs. 77.1%; P = 0.059). There was no difference between fresh legumes for CH4 emissions [25.9 g/kg DMI ± 4.02 SE; 8.5% of gross energy intake (GEI) ± 1.26 SE; or 36.8 g/kg digested OM ± 1.75 SE]. The fresh legumes were more digestible at early, rather than at late, maturity and, consequently, enteric CH4 (27.4 vs. 24.4 g/kg DMI; P emissions (22.4 g/kg DMI ± 1.29 SD and 7.1% GEI ± 0.40 SD). However, on the basis of OM digested, CH4 emissions were lower for sainfoin than alfalfa hay (44.3 vs. 59.0 g/kg; P = 0.008). Percentage of total N excretion in urine was less for sainfoin compared with alfalfa, both for fresh legumes in Exp. 1 (74 vs. 78%; P = 0.017) or hay in Exp. 2 (64 vs. 72%; P emissions from beef cattle fed at maintenance as compared with feeding either 80% alfalfa:20% sainfoin (fresh forages) or 100% alfalfa (hay). Feeding fresh legumes harvested between the late vegetative to early bud stage, compared with

  13. Effect of quantity, quality, and length of alfalfa hay on selective consumption by dairy cows.

    Science.gov (United States)

    Leonardi, C; Armentano, L E

    2003-02-01

    Twenty-four lactating Holstein cows were used in a replicated 6 x 6 Latin square design. Experimental periods were 6 or 7 d. Cows were housed in tie-stalls, and diets were fed ad libitum twice daily at 1100 and 1600 h. Diets contained 60% concentrate and either 40% alfalfa hay or 20% alfalfa hay and 20% alfalfa silage (dry matter basis). The effect of quantity, quality, and length of hay on sorting behavior was determined. Treatments consisted of 20% lower or higher quality long alfalfa hay, 20% lower or higher quality chopped alfalfa hay, and 40% lower or higher quality chopped alfalfa hay. Variation of sorting among cows was also determined. Particle size distribution of samples of as-fed total mixed rations and orts were determined using the Wisconsin particle size separator. Screens have square holes with diagonals of 26.9, 18, 8.98, 5.61, and 1.65 mm (screens Y1 to Y5, respectively). Sorting was calculated as the actual intake of each fraction expressed as a percentage ofthe predicted intake. Increasing the proportion of dry hay increased sorting. Quality of alfalfa hays that were offered did not affect sorting activity. Feeding long alfalfa hay increased selective consumption of fine particles. However, feeding long alfalfa hay also increased intake of longer particles because a higher percentage of long particles was offered. Across treatments, animals consistently sorted against longer particles in favor of finer particles. In particular, intake of Y1 as a percentage of the predicted intake was the most variable. Average Y1 intake, across the six treatments for each cow, was between 60 and 70% of predicted intake for four cows, 71 to 80% for 11 cows, 81 to 90% for five cows, 91 to 100% for two cows, and 101 to 110% for two cows. On one diet a cow failed to consume any of the Y1 portion of the total mixed ration. This variation among animals in sorting of very long feed particles may have practical significance.

  14. 7 CFR 202.44 - Proceedings under section 305(b) to determine whether foreign alfalfa or red clover seed is not...

    Science.gov (United States)

    2010-01-01

    ... foreign alfalfa or red clover seed is not adapted for general agricultural use in the United States. 202... Proceedings under section 305(b) to determine whether foreign alfalfa or red clover seed is not adapted for... for the purpose of determining whether seed of alfalfa or red clover from any foreign country...

  15. Occurrence of alfalfa (Medicago sativa L.) populations along roadsides in southern Manitoba, Canada and their potential role in intraspecific gene flow.

    Science.gov (United States)

    Bagavathiannan, Muthukumar V; Gulden, Robert H; Van Acker, Rene C

    2011-04-01

    Alfalfa is a highly outcrossing perennial species that can be noticed in roadsides as feral populations. There remains little information available on the extent of feral alfalfa populations in western Canadian prairies and their role in gene flow. The main objectives of this study were (a) to document the occurrence of feral alfalfa populations, and (b) to estimate the levels of outcrossing facilitated by feral populations. A roadside survey confirmed widespread occurrence of feral alfalfa populations, particularly in alfalfa growing regions. The feral populations were dynamic and their frequency ranged from 0.2 to 1.7 populations km(-1). In many cases, the nearest feral alfalfa population from alfalfa production field was located within a distance sufficient for outcrossing in alfalfa. The gene flow study confirmed that genes can move back and forth between feral and cultivated alfalfa populations. In this study, the estimated outcrossing levels were 62% (seed fields to feral), 78% (feral to seed fields), 82% (hay fields to feral) and 85% (feral to feral). Overall, the results show that feral alfalfa plants are prevalent in alfalfa producing regions in western Canada and they can serve as bridges for gene flow at landscape level. Management of feral populations should be considered, if gene flow is a concern. Emphasis on preventing seed spill/escapes and intentional roadside planting of alfalfa cultivars will be particularly helpful. Further, realistic and pragmatic threshold levels should be established for markets sensitive to the presence of GE traits.

  16. Accumulation and residue of napropamide in alfalfa (Medicago sativa) and soil involved in toxic response.

    Science.gov (United States)

    Cui, Li E; Yang, Hong

    2011-06-15

    Napropamide belongs to the amide herbicide family and widely used to control weeds in farmland. Intensive use of the herbicide has resulted in widespread contamination to ecosystems. The present study demonstrated an analysis on accumulation of the toxic pesticide napropamide in six genotypes of alfalfa (Medicago sativa), along with biological parameters and its residues in soils. Soil was treated with napropamide at 3 mg kg(-1) dry soil and alfalfa plants were cultured for 10 or 30 d, respectively. The maximum value for napropamide accumulation is 0.426 mg kg(-1) in shoots and 2.444 mg kg(-1) in roots. The napropamide-contaminated soil with alfalfa cultivation had much lower napropamide concentrations than the control (soil without alfalfa cultivation). Also, the content of napropamide residue in the rhizosphere was significantly lower than that in the non-rhizosphere soil. M. sativa exposed to 3 mg kg(-1) napropamide showed inhibited growth. Further analysis revealed that plants treated with napropamide accumulated more reactive oxygen species (O(2)(-) and H(2)O(2)) and less amounts of chlorophyll. However, not all cultivars showed oxidative injury, suggesting that the alfalfa cultivars display different tolerance to napropamide.

  17. Yield, chemical composition and persistence of alfalfa on moderately acidic mountain soil

    Directory of Open Access Journals (Sweden)

    Josip Leto

    2006-12-01

    Full Text Available Due to its excellent nutritional characteristics and high yields, alfalfa is the most important forage crop in roughage production. The main limiting factor in global food production is soil acidification. At the moment, about 40% of world agricultural soils are acidic. It is difficult to grow alfalfa on acid soils (pH 0.05. Average DM yield of all cultivars in the year 2000 was 7.07 t/ha, in the year 2001 it was 10.94 t/ha, and finally in the year 2002 it was 12.78 t/ha. Significant differences in DM yields were recorded between cuttings (P0.05. Mean crude protein content was 28.2%, while contents of crude fat, crude fibers and non nitrogen free extract (NFE were 3.73%, 16.15%, 29.19%, respectively. No significant differences in alfalfa ground cover were recorded between cultivars in autumn or in spring in all experimental years (P>0.05. Significant differences in alfalfa ground cover in autumn (P<0.05 and in spring (P<0.01 were recorded between years. The lowest average ground cover was recorded in last experimental year: in the autumn of the year 2001(72.81% and in the spring of the year 2002 (64.37%. All investigated alfalfa cultivars are suitable for growing in similar agroecological conditions.

  18. Effect of aflatoxin B1 on in vitro ruminal fermentation of rations high in alfalfa hay or ryegrass hay

    DEFF Research Database (Denmark)

    Jiang, Y H; Yang, H J; Lund, Peter

    2012-01-01

    A 2 × 4 factorial experiment was conducted to determine the effect of aflatoxin B1 (AFB1) at dose rates of 0, 320, 640, 960 ng/ml on ruminal fermentation of substrates high in alfalfa hay (HA, alfalfa hay: maize meal = 4:1) and ryegrass hay (HR, ryegrass hay: maize meal = 4:1). In vitro dry matter...

  19. In vitro ruminal fermentation of treated alfalfa silage using ruminal inocula from high and low feed-efficient lactating cows

    Science.gov (United States)

    The objective was to assess the effect of two additives on alfalfa silage and on in vitro ruminal fermentation when using ruminal inocula prepared from high feed-efficient (HE) and low feed-efficient (LE) lactating cows. Second and third cut alfalfa was harvested at 40% bloom stage, treated with con...

  20. The genetic components of extended life expectancy in chilled, post-diapause quiescent Alfalfa Leafcutting Bees, Megachile rotundata

    Science.gov (United States)

    The alfalfa leafcutting bee, Megachile rotundata, a solitary bee native to Eurasia, is the world’s most intensively managed solitary bee and has become the primary pollinator for alfalfa seed production. These bees, when commercially managed, are overwintered as diapausing prepupae under static ther...

  1. Screening of Highly Effective Sinorhizobium meliloti Strains for 'Vector' Alfalfa and Testing of Its Competitive Nodulation Ability in the Field

    Institute of Scientific and Technical Information of China (English)

    ZENG Zhao-Hai; CHEN Wen-Xin; HU Yue-Gao; SUI Xin-Hua; CHEN Dan-Ming

    2007-01-01

    Seventeen Sinorhizobium meliloti strains from seven provinces in China were used to screen highly effective strains for alfalfa cultivar in a greenhouse study and their symbiotic relationship and competitive ability were studied in the field.CCBAU30138 was the most effective strain,as evidenced by increase in dry weights.A field experiment showed that the inoculation of alfalfa with CCBAU30138 resulted in increases of 11.9%and 19.6%of dry matter production and crude protein production,respectively,in forage of monocultured plants.The total dry matter yields of alfalfa and tall fescue in binary culture were increased by 16.3%by inoculation of alfalfa with this strain.These results showed that S.Meliloti strain CCBAU30138 was an effective inoculant both in the greenhouse and in the field.The analysis of randomly amplified polymorphic DNA(RAPD)by polymerase chain reaction(PCR)from nodule extracts showed that the strain CCBAU30138 had high competitiveness in the field.It occupied 47.5%of nodules in alfalfa monoculture and 44.4% of nodules in alfalfa-tall fescue binary culture after 20 weeks of growth.In conclusion,a simple system to select highly effective and competitive symbiotic strains specific to alfalfa was established.Using this system.A strain suitable for the alfalfa cultivar'Vector’grown in Wuqiao County of Hebei Province was obtained.

  2. Analysis of the UV-B Regime and Potential Effects on Alfalfa

    Science.gov (United States)

    Seitz, Jeffery C.

    1998-01-01

    Life at the surface of the Earth, over the last 400 m.y., evolved under conditions of decreased short-wave radiation (i.e., ultraviolet) relative to solar output due to absorption and scattering by constituents (e.g., ozone, water vapor, aerosols) in the upper atmosphere. However, a significant amount of ultraviolet radiation in the range from 280-320 nm, known as ultraviolet-B radiation, reaches the Earth's surface and has sufficient energy to be damaging to biologic tissue. Natural fluctuations in atmospheric constituents (seasonal variation, volcanic eruptions, etc.), changes in the orbital attitude of the Earth (precession, axial tilt, orbital eccentricity), and long-term solar variability contribute to changes in the total amount of ultraviolet radiation reaching the surface of the Earth, and thus, the biosphere. More recently, the atmospheric release of commercial propellants and refrigerants, known as chlorofluorocarbons (CFCs), has contributed to a significant depletion in naturally occurring ozone in the stratosphere. Thus, decreased stratospheric ozone has resulted in an increased UV-B flux at the Earth's surface which may have profound effects on terrestrial and marine organisms. In this study, we are investigating the effects of differing solar UV-B fluxes on alfalfa (Medicago sativa L.), an important agricultural crop. A long-term goal of this research is to develop spectral signatures to detect plant response to increased UV-B radiation from remote sensor platforms.

  3. Molecular cloning and characterization of a gene regulating flowering time from Alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Zhang, Tiejun; Chao, Yuehui; Kang, Junmei; Ding, Wang; Yang, Qingchuan

    2013-07-01

    Genes that regulate flowering time play crucial roles in plant development and biomass formation. Based on the cDNA sequence of Medicago truncatula (accession no. AY690425), the LFY gene of alfalfa was cloned. Sequence similarity analysis revealed high homology with FLO/LFY family genes of other plants. When fused to the green fluorescent protein, MsLFY protein was localized in the nucleus of onion (Allium cepa L.) epidermal cells. The RT-qPCR analysis of MsLFY expression patterns showed that the expression of MsLFY gene was at a low level in roots, stems, leaves and pods, and the expression level in floral buds was the highest. The expression of MsLFY was induced by GA3 and long photoperiod. Plant expression vector was constructed and transformed into Arabidopsis by the agrobacterium-mediated methods. PCR amplification with the transgenic Arabidopsis genome DNA indicated that MsLFY gene had integrated in Arabidopsis genome. Overexpression of MsLFY specifically caused early flowering under long day conditions compared with non-transgenic plants. These results indicated MsLFY played roles in promoting flowering time.

  4. Characterization of the Newly Developed Soybean Cultivar DT2008 in Relation to the Model Variety W82 Reveals a New Genetic Resource for Comparative and Functional Genomics for Improved Drought Tolerance

    Directory of Open Access Journals (Sweden)

    Chien Van Ha

    2013-01-01

    Full Text Available Soybean (Glycine max productivity is adversely affected by drought stress worldwide, including Vietnam. In the last few years, we have made a great effort in the development of drought-tolerant soybean cultivars by breeding and/or radiation-induced mutagenesis. One of the newly developed cultivars, the DT2008, showed enhanced drought tolerance and stable yield in the field conditions. The purpose of this study was to compare the drought-tolerant phenotype of DT2008 and Williams 82 (W82 by assessing their water loss and growth rate under dehydration and/or drought stress conditions as a means to provide genetic resources for further comparative and functional genomics. We found that DT2008 had reduced water loss under both dehydration and drought stresses in comparison with W82. The examination of root and shoot growths of DT2008 and W82 under both normal and drought conditions indicated that DT2008 maintains a better shoot and root growth rates than W82 under both two growth conditions. These results together suggest that DT2008 has better drought tolerance degree than W82. Our results open the way for further comparison of DT2008 and W82 at molecular levels by advanced omic approaches to identify mutation(s involved in the enhancement of drought tolerance of DT2008, contributing to our understanding of drought tolerance mechanisms in soybean. Mutation(s identified are potential candidates for genetic engineering of elite soybean varieties to improve drought tolerance and biomass.

  5. Overexpression of a Cytosolic Ascorbate Peroxidase Gene, OsAPX2, Increases Salt Tolerance in Transgenic Alfalfa

    Institute of Scientific and Technical Information of China (English)

    ZHANG Qian; MA Cui; XUE Xin; XU Ming; LI Jing; WU Jin-xia

    2014-01-01

    Alfalfa (Medicago sativa L.) is an important forage crop in the world and it is of great signiifcance for the improvement of its salt tolerance. To improve salt tolerance in alfalfa, a rice ascorbate peroxidase gene (OsAPX2) was introduced into alfalfa using Agrobacterium tumefaciens-mediated transformation with marker gene bar. The different T-DNA insertions in T1 transgenic alfalfa were identiifed by Southern hybridization. Three independent T2 transgenic lines were selected for stress analysis and the results showed that all of them were salt tolerant compared with wild-type plants. The transgenic plants had low levels of H2O2, malondialdehyde and relative electrical conductivity under salt and drought stresses. Moreover, the contents of chlorophyll and proline, and APX activity were high in transgenic plants under salt and drought stresses. Taken together, the overexpression of OsAPX2 enhances salt tolerance in alfalfa through scavenging reactive oxygen species.

  6. The Undergraduate ALFALFA Team: A Model for Involving Undergraduates in Large Astronomy Collaborations

    Science.gov (United States)

    Craig, David W.; Koopmann, Rebecca A.; Haynes, Martha P.; Undergraduate ALFALFA Team, ALFALFA Team

    2016-01-01

    The NSF-sponsored Undergraduate ALFALFA (Arecibo Legacy Fast ALFA) Team (UAT) has allowed faculty and students from a wide range of public and private colleges and especially those with small astronomy programs to learn how science is accomplished in a large collaboration while contributing to the scientific goals of a legacy radio astronomy survey. This effort has been made possible through the collaboration of the ALFALFA PIs and graduate students, Arecibo Observatory staff, and the faculty at 19 undergraduate-focussed institutions. In this talk, we will discuss how the UAT model works for the ALFALFA project and lessons learned from our efforts over the 8 years of grant funding. We will provide suggestions on how the model could be applied to other legacy projects, particularly in such areas as online collaboration and software usage by undergraduates. This work has been supported by NSF grants AST-0724918/0902211, AST-075267/0903394, AST-0725380, and AST-1211005.

  7. The Properties of Galaxies in Low Density Regions from the ALFALFA Survey

    CERN Document Server

    Toribio, M C

    2009-01-01

    Galaxies detected in the 21-cm line of neutral hydrogen (HI) from the on-going Arecibo Legacy Fast ALFA (ALFALFA) blind extragalactic HI survey have been cross-correlated with Sloan Digital Sky Survey Data Release 7 (Abazajian et al. 2009) in order to define a reference sample of HI content in regions of low galactic density. This observational sample will be used in the future to derive new standards of normal atomic gas content that allow a statistical investigation of the HI properties of galaxies in differing environments of the local universe. As a previous step, we compare here morphological indicators, like color or light concentration index, of ALFALFA detections and non-detections in low density regions. Our examination is extended also to a small data set of isolated galaxies. This kind of analysis is necessary in order to characterize as accurately as possible the type of galaxies that ALFALFA is detecting.

  8. The Properties of Galaxies in Low Density Regions from the ALFALFA Survey

    Science.gov (United States)

    Toribio, M. C.; Solanes, J. M.; Alfalfa Collaboration

    2010-10-01

    Galaxies detected in the 21-cm line of neutral hydrogen (H I) from the on-going Arecibo Legacy Fast ALFA (ALFALFA) blind extragalactic H I survey have been cross-correlated with Sloan Digital Sky Survey (SDSS) Data Release 7 (Abazajian et al. 2009) in order to define a reference sample of H I content in regions of low galactic density. This observational sample will be used in the future to derive new standards of normal atomic gas content that allow a statistical investigation of the H I properties of galaxies in differing environments of the local universe. As a previous step, we compare here morphological indicators, like color or light concentration index, of ALFALFA detections and non-detections in low density regions. Our examination is extended also to a small data set of isolated galaxies. This kind of analysis is necessary in order to characterize as accurately as possible the type of galaxies that ALFALFA is detecting.

  9. 北京花卉种苗产业现状分析与发展策略%Present Situation Analysis and Development Strategy on Flower Seedling Industrialization of Popular Flowers Variety in Beijing

    Institute of Scientific and Technical Information of China (English)

    李子敬; 陈晓; 董爱香; 衣彩洁; 丛日晨

    2015-01-01

    笔者论述了种苗产业化的主要组成要素,并综合分析国内外种苗产业现状,分析认为北京种苗产业发展的问题主要涉及缺乏自主知识产权新品种、产业链条不完善、法律法规不健全、销售模式单一化、服务和标准体系不完善、产学研联合体不健全等方面,并有针对性地提出了发展北京种苗产业的发展策略,主要包括转变政府职能并加强调控、发展北京特色花卉品种、细化产业分工、构建花卉种苗质量标准体系和认证机构、开展服务体系建设及建立产学研综合体等主要对策等,旨在为北京市种苗产业化发展提供决策依据。%Essential factors of flower seedling industrialization were discussed in this paper. The results indicated that the problems of flower industry in Beijing were deficiency of independent intellectual flower variety, incomplete flower industrial linking, unsoundness of law and regulation, simple model of sale, imperfect system of service and standard, and deficient of production-college-research union. Strategies of developing flower seedling industry were suggested, such us reinforcing government regulation, cultivating Beijing-characteristic flower variety, refining industrial division, establishment of flower seedlings quality standard system and certification institutions, developing service system and establishing flower seedling consortium, on the base of the analysis of international flower seedling situation, in order to provide policy-decision basis for flower seedling industrialization.

  10. Effects of plant enzyme inactivation or sterilization on lipolysis and proteolysis in alfalfa silage.

    Science.gov (United States)

    Ding, W R; Long, R J; Guo, X S

    2013-04-01

    This experiment studied the contribution of plant enzymes and microbial activity on lipolysis and proteolysis in ensiled alfalfa. Before ensiling, the wilted alfalfa was treated with plant enzyme inactivation by autoclaving or with sterilization by γ-ray irradiation. The treated alfalfa was then inoculated with commercial lactic acid bacteria inoculants and ensiled for 40 d. Alfalfa without treatment was ensiled as the control. The content of total fatty acid (FA) after ensiling decreased 43% in the control silage and 28% in the γ-ray-treated silage, but did not change in the autoclave-treated silage. Among the major FA (C16:0, C18:2n-6, C18:3n-3), a considerable increase was observed in proportion of C16:0 in the control silage as compared with fresh alfalfa; conversely, decreases in proportions of C18:2n-6 and C18:3n-3 occurred during ensilage. Silage treated with γ-ray radiation at ensiling had a smaller proportion of C16:0 and greater proportions of C18:2n-6 and C18:3n-3 than control silage. Autoclave treatment further decreased proportions of C16:0 and most of the other FA, and increased C18:2n-6 and C18:3n-3 proportions in comparison with γ-ray treatment. Proportions of C16:0, C18:2n-6, C18:3n-3 and other detected FA (except for the proportion of C15:0) did not differ between fresh forage and autoclave-treated silage. Remarkably, smaller nonprotein nitrogen content was observed in the autoclave-treated silage compared with the γ ray-treated silage or the control silage. These results indicated that an extensive lipolysis occurred during ensiling of alfalfa, and plant enzymes played a major role in lipolysis and proteolysis.

  11. Assessing the Spatial Variability of Alfalfa Yield Using Satellite Imagery and Ground-Based Data

    Science.gov (United States)

    Kayad, Ahmed G.; Al-Gaadi, Khalid A.; Tola, ElKamil; Madugundu, Rangaswamy; Zeyada, Ahmed M.; Kalaitzidis, Chariton

    2016-01-01

    Understanding the temporal and spatial variability in a crop yield is viewed as one of the key steps in the implementation of precision agriculture practices. Therefore, a study on a center pivot irrigated 23.5 ha field in Saudi Arabia was conducted to assess the variability in alfalfa yield using Landsat-8 imagery and a hay yield monitor data. In addition, the study was designed to also explore the potential of predicting the alfalfa yield using vegetation indices. A calibrated yield monitor mounted on a large rectangular hay baler was used to measure the actual alfalfa yield for four alfalfa harvests performed in the period from October 2013 to May 2014. A total of 18 Landsat-8 images, representing different crop growth stages, were used to derive different vegetation indices (VIs). Data from the yield monitor was used to generate yield maps, which illustrated a definite spatial variation in alfalfa yield across the experimental field for the four studied harvests as indicated by the high spatial correlation values (0.75 to 0.97) and the low P-values (4.7E-103 to 8.9E-27). The yield monitor-measured alfalfa actual yield was compared to the predicted yield form the Vis. Results of the study showed that there was a correlation between actual and predicted yield. The highest correlations were observed between actual yield and the predicted using NIR reflectance, SAVI and NDVI with maximum correlation coefficients of 0.69, 0.68 and 0.63, respectively. PMID:27281189

  12. Gel Electrophoresis of Proteins for the Identification of Crop Varieties

    Institute of Scientific and Technical Information of China (English)

    LAN Hai-yan; LI Li-hui

    2002-01-01

    With the development of the international trade and agricultural science and technology, especially after the execution of the rules on protection of new plant varieties, considerable emphasis has been placed on variety identification. Many evidences have suggested that gel electrophoresis have great influence on this area. This paper reviewed study status of various gel electrophoresis, including development of the methods, comparison of these techniques, influence factors, practical applications, achievements obtained and aspects in the future study. With the wider range on protection of new plant varieties in China, electrophoresis will play a more important role in variety identification.

  13. Black Leaf and Stem of Alfalfa Caused by Phoma medicaginis Malbr & Roum. var. medicaginis Boerema

    Directory of Open Access Journals (Sweden)

    Latif R. Susuri

    2003-03-01

    Full Text Available Phoma medicaginis has recently been spreading on alfalfa causing black leaf and stem in irrigation fields near Prizren. The average number of lesions is from 18 up to 30 on leaves with an average diameter of 1-10 mm. The average size of pycnidia from diseased alfalfa leaves, stems and PDA is 325 x 338 μm, whereas the average size of pycnidiospores is 7.3 x 3.6 μm. Maximum spore production in average was on PDA 140.9 x 104/ml.

  14. Evaluación de 14 variedades de alfalfa con fertirriego en la Mixteca de Oaxaca

    OpenAIRE

    Joel Morales Ayala; José Luis Jiménez Victoria; Vicente Arturo Velasco Velasco; Yuri Villegas Aparicio; José Raymundo Enríquez del Valle; Alfonso Hernández Garay

    2006-01-01

    Se evaluaron 14 variedades de alfalfa con el sistema de fertirriego por goteo, con la finalidad de determinar la variedad de mayor rendimiento en materia seca, relación hoja:tallo y altura de planta, y la comparación de dos variedades de alfalfa mediante el sistema de riego por gravedad para evaluar el consumo de agua y energía eléctrica. El estudio se estableció en el Campo Experimental Mixteca Oaxaqueña, perteneciente al INIFAP, Yanhuitlán, Nochixtlán, Oaxaca. La siembra se realizó con una ...

  15. Evolutionary relationship of alfalfa mosaic virus with cucumber mosaic virus and brome mosaic virus

    OpenAIRE

    Savithri, HS; Murthy, MRN

    1983-01-01

    The amino acid sequences of the non-structural protein (molecular weight 35,000; 3a protein) from three plant viruses - cucumber mosaic, brome mosaic and alfalfa mosaic have been systematically compared using the partial genomic sequences for these three viruses already available. The 3a protein of cucumber mosaic virus has an amino acid sequence homology of 33.7% with the corresponding protein of brome mosaic virus. A similar protein from alfalfa mosaic virus has a homology of 18.2% and 14.2...

  16. 黄河流域棉花品种在江西棉区的生长发育分析%Analysis of the Growth and Development of Cotton Varieties from Yellow River Basin in Jiangxi Cotton Region

    Institute of Scientific and Technical Information of China (English)

    夏绍南; 陈宜; 张丽娟; 李永旗; 崔爱花; 高红兵; 李直兴; 谢业涛

    2015-01-01

    In order to study the adaptability of different maturity cotton varieties in Poyang Lake District,the theoretical basis for selecting suitable cotton varieties in the region was improved.In 2014,the author designed 8 varieties of 3 kinds of maturity and analysed the differences from the characteristics of leaf area,specific leaf weight,leaf area index (LAI),drymatter accumulation and yield and so on.The results showed that:1 .the main stem leaf area had significantly differences among the varieties;2.the difference of specific leaf weight was not significant in the early maturity,the late (flowering stage, topping stage,boll opening stage)had obvious difference;3.the main stem leaf area was less than fruit leaf area in the flow-ering and boll opening stage,and early maturing varieties were earlier than the middle maturing;4.the difference of stage was larger after flowering stage,early maturing varieties varieties varieties;5.the distri-bution of dry matter in each investigation period was similar,period,and dry matter accumulation was early maturing varietiesvarieties varieties;6.the yield was early maturing varieties vari-eties varieties.Early maturing varieties as long as increased the density and enhanced the LAI value, expanding the source,could get a high yield.At present,under the condition of planting mid -early maturing varieties were more suitable for Poyang Lake cotton region,and as the first ripe varieties.%为研究不同熟性棉花品种在鄱阳湖区的适应性情况,完善该地区选择适宜棉花熟性品种提供理论依据。于2014年设置了3种熟性8个品种比较试验,从叶面积、比叶重、叶面积指数(LAI)、干物质积累及产量等性状表现分析其差异。结果表明:一是主茎倒四叶叶面积各品种间差异明显;二是比叶重在熟性间前期差异不明显,后期(盛花期

  17. Minimizing the time and cost of production of transgenic alfalfa libraries using the highly efficient completely sequenced vector pPZP200BAR.

    Science.gov (United States)

    Jozefkowicz, Cintia; Bottero, Emilia; Pascuan, Cecilia; Pagano, Elba; Ayub, Nicolás Daniel; Soto, Gabriela

    2016-09-01

    Alfalfa is the most important forage legume worldwide. However, similar to other minor forage crops, it is usually harvested along with weeds, which decrease its nutrient quality and thus reduce its high value in the market. In addition, weeds reduce alfalfa yield by about 50 %. Although weeds are the limiting factor for alfalfa production, little progress has been made in the incorporation of herbicide-tolerant traits into commercial alfalfa. This is partially due to the high times and costs needed for the production of vast numbers of transgenic alfalfa events as an empirical approach to bypass the random transgenic silencing and for the identification of an event with optimal transgene expression. In this focus article, we report the complete sequence of pPZP200BAR and the extremely high efficiency of this binary vector in alfalfa transformation, opening the way for rapid and inexpensive production of transgenic events for alfalfa improvement public programs.

  18. Identification of the Changing Educational Needs of Midwives in Developing New Dimensions of Care in a Variety of Settings and the Development of an Educational Package To Meet These Needs.

    Science.gov (United States)

    Pope, Rosemary; And Others

    The changing educational needs of midwives in England were assessed, and an open learning package for midwives was developed based on the findings. Data were collected from the following: questionnaires mailed to 1,100 registered midwives (70% response rate), all 205 coordinating supervisors of midwives in England (83% response rate); 1 randomly…

  19. Algebra-Geometry of Piecewise Algebraic Varieties

    Institute of Scientific and Technical Information of China (English)

    Chun Gang ZHU; Ren Hong WANG

    2012-01-01

    Algebraic variety is the most important subject in classical algebraic geometry.As the zero set of multivariate splines,the piecewise algebraic variety is a kind generalization of the classical algebraic variety.This paper studies the correspondence between spline ideals and piecewise algebraic varieties based on the knowledge of algebraic geometry and multivariate splines.

  20. Assortment Variety : Attribute versus Product-Based

    NARCIS (Netherlands)

    van Herpen, H.W.I.; Pieters, R.

    2000-01-01

    Retailers need to decide on the content and structure of their product assortments, and thereby on the degree of variety that they offer to their customers.This paper compares measures of assortment variety and relates them to underlying variety components.We conceptualize assortment variety from a

  1. Simple MCA supports a variety of applications

    Energy Technology Data Exchange (ETDEWEB)

    Wolf, M.A.; Staley, H.C.

    1984-01-01

    A greatly enhanced version of the Violinist I, Simple Multichannel analyzer has been developed at Los Alamos. The new instrument includes a more powerful microprocessor, more memory, and a sophisticated set of I/O devices. The combination of these features allow the same basic electronics package to function in a variety of smart sophisticated monitoring instruments. Only the packaging, detector, and software need be changed to change the instrument from a field survey instrument for Pu contamination to a continuous air monitor to a neutron dosimeter. This instrument should spawn a new generation of user-friendly, highly effective instrumentation.

  2. Simple MCA supports a variety of applications

    Energy Technology Data Exchange (ETDEWEB)

    Wolf, M.A.; Staley, H.C.

    1985-02-01

    A greatly enhanced version of the Violinist I, Simple Multichannel Analyzer has been developed at Los Alamos. The new instrument includes a more powerful microprocessor, more memory, and a sophisticated set of I/O devices. The combination of these features allow the same basic electronics package to function in a variety of ''smart'' sophisticated monitoring instruments. Only the packaging, detector, and software need be changed to change the instrument from a field survey instrument for Pu contamination to a continuous air monitor to a neutron dosimeter. This instrument should spawn a new generation of user-friendly, highly effective instrumentation.

  3. Increase phosphorus availability from the use of alfalfa (Medicago sativa L) green manure in rice (Oryza sativa L.) agroecosystem.

    Science.gov (United States)

    Gao, Xiaoye; Shi, Dongyan; Lv, Aimin; Wang, Shengyin; Yuan, Shili; Zhou, Peng; An, Yuan

    2016-11-11

    Alfalfa is a good green manure source, but its effect on rice growth has not been fully elucidated. Two green manure species, alfalfa and broad bean (Vicia faba L.), and two N fertilizer levels, alone or combination, were applied to a rice field. The results indicated that alfalfa had more pronounced effects on increasing soil labile phosphorus (P) fractions (including NaHCO3-Pi, NaOH-Pi), P uptake and soil enzyme activities (dehydrogenase, urease, acid phosphatase and β-glucosidase) than broad bean and N fertilizer. The transformation of NaHCO3-Po to labile P regulated by alfalfa played a significant direct and indirect effect on grain yield. Although a much lower N input from alfalfa addition, a similar grain yield with N fertilizer treatment was achieved, and the integration of alfalfa with N fertilizer produced the highest grain yield and P availability, which was associated with the highest urease, acid phosphatase and β-glucosidase activity in soil. These results indicate that alfalfa green manure had a great ability of increasing grain yield through enhancing P availability in rice paddy, which could give us a way to reduce N fertilizer application by enhancing P availability.

  4. Processed Methods of Alfalfa in Rainy Season%雨季苜蓿草加工的几种途径

    Institute of Scientific and Technical Information of China (English)

    向金城; 曹致中; 仇亨

    2014-01-01

    针对苜蓿草在雨季容易发霉腐烂的问题,笔者提出了青贮、烘干、榨汁后烘干及汁液提取叶蛋白等可行的加工贮存途径。青贮中不论是否添加玉米粉、乳酸、乙酸及乳酸菌接种剂,只要晾晒至半干(含水量50%~60%)铡碎、压紧、隔绝空气,进行半干青贮,都能获得优质青贮苜蓿。%Feasible approaches to preventing alfalfa from decaying during wet seasons were proposed. These approaches include making alfalfa silage, making alfalfa hay and making hay after the juice in alfalfa is extracted. The extracted juice from alfalfa is used to make leaf proteins. When making the alfalfa silage, it is not important whether corn lfour, lactic acid, acetic acid or lactic acid bacteria inoculant are added in the raw materials, as long as alfalfa foliage is pre-dried ( 50%~60%water content), ifnely chopped and ifrmly pressed (to remove air), the high quality foliage/semi-foliage can always be achieved.

  5. Increase phosphorus availability from the use of alfalfa (Medicago sativa L) green manure in rice (Oryza sativa L.) agroecosystem

    Science.gov (United States)

    Gao, Xiaoye; Shi, Dongyan; Lv, Aimin; Wang, Shengyin; Yuan, Shili; Zhou, Peng; An, Yuan

    2016-01-01

    Alfalfa is a good green manure source, but its effect on rice growth has not been fully elucidated. Two green manure species, alfalfa and broad bean (Vicia faba L.), and two N fertilizer levels, alone or combination, were applied to a rice field. The results indicated that alfalfa had more pronounced effects on increasing soil labile phosphorus (P) fractions (including NaHCO3-Pi, NaOH-Pi), P uptake and soil enzyme activities (dehydrogenase, urease, acid phosphatase and β-glucosidase) than broad bean and N fertilizer. The transformation of NaHCO3-Po to labile P regulated by alfalfa played a significant direct and indirect effect on grain yield. Although a much lower N input from alfalfa addition, a similar grain yield with N fertilizer treatment was achieved, and the integration of alfalfa with N fertilizer produced the highest grain yield and P availability, which was associated with the highest urease, acid phosphatase and β-glucosidase activity in soil. These results indicate that alfalfa green manure had a great ability of increasing grain yield through enhancing P availability in rice paddy, which could give us a way to reduce N fertilizer application by enhancing P availability. PMID:27833163

  6. Streptomyces alfalfae sp. nov. and comparisons with its closest taxa Streptomyces silaceus, Streptomyces flavofungini and Streptomyces intermedius.

    Science.gov (United States)

    She, Wenqing; Sun, Zhongfeng; Yi, Lei; Zhao, Shumiao; Liang, Yunxiang

    2016-01-01

    A novel streptomycete strain, designated XY25T, was isolated from the rhizosphere soil in an alfalfa field in Jingyang, Shanxi, China. The isolate showed optimal growth at 37 °C, and was capable of growing at pH 6-10 and in the presence of 0-6 % (w/v) NaCl. Mycelia of strain XY25T appeared spiral and developed into white spore chains with long-rod spores and a smooth surface. The 16S rRNA gene sequence of XY25T was determined and was found to be highly similar to those of species of the genus Streptomyces including Streptomyces silaceus DSM 41861T (99.11 % 16S rRNA gene sequence similarity), Streptomyces flavofungini DSM 40366T (98.49 %) and Streptomyces intermedius DSM 40372T (98.43 %), all of which were used for further characterization. Each of the four streptomycetes showed distinctive patterns of carbon usage and fatty acids composition. Analysis of cellular components of strain XY25T revealed ll-diaminopimelic acid as diagnostic diamino acid and xylose as the major sugar, whereas polar lipids were determined as phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid, two unknown phosphatidylinositol mannosides and several unknown lipids. Menaquinones were dominated by MK-9(H6) and MK-9(H8), and the main fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. DNA-DNA hybridization studies indicated that strain XY25T showed relatedness values of 35.2-40.42 % with the closest related species. Based on these results, strain XY25T represents a novel species of the genus Streptomyces, for which the name Streptomyces alfalfae sp. nov. is proposed. The type strain is XY25T ( = KCTC 39571T = CCTCC AA2015019T).

  7. 78 FR 37200 - Plant Variety Protection Board; Open Teleconference Meeting

    Science.gov (United States)

    2013-06-20

    ..., electronic PVP application/computer database development, and the use of molecular markers for PVP... Agricultural Marketing Service Plant Variety Protection Board; Open Teleconference Meeting AGENCY: Agricultural... their opportunity to attend an open meeting of the Plant Variety Protection Board. DATES: July 31,...

  8. Proteomic analysis of salt and osmotic-drought stress in alfalfa seedlings

    Institute of Scientific and Technical Information of China (English)

    MA Qiao-li; KANG Jun-mei; LONG Rui-cai; CUI Yan-jun; ZHANG Tie-jun; XIONG Jun-bo; YANG Qing-chuan; SUN Yan

    2016-01-01

    Alfalfa is widely grown and is one of the most important forage crops in the world, but its growth and biomass production are markedly reduced under salt and drought stress, particularly during the early seedling stage. To identify the mecha-nisms behind salt and drought responsiveness at the alfalfa seedling stage, the proteins expressed were analyzed under no-treatment, 200 mol L–1 NaCl and 180 g L–1 PEG treatment conditions during the seedling stage. Out of more than 800 protein spots detected on two-dimensional electrophoresis (2-DE) gels, 35 proteins showed statisticaly signiifcant responses (P<0.05) to NaCl and PEG stress, which were selected for tandem mass spectrometric identiifcation, owing to their good resolution and abundance levels, and 32 proteins were positively identiifed. The identiifed proteins were divided into seven functional categories: photosynthetic metabolism, protein biosynthesis, folding and assembly, carbohydrate metabolism-associated proteins, stress defense related protein, metabolism of nucleic acid, other function categories and unknown proteins. Our results suggested that these proteins may play roles in alfalfa adaptation to salt and drought stress. Further study of these proteins wil provide insights into the molecular mechanisms of abiotic stress and the discovery of new candidate markers in alfalfa.

  9. Population Dynamics of Empoasca fabae (Hemiptera: Cicadellidae) in Central Iowa Alfalfa Fields.

    Science.gov (United States)

    Weiser Erlandson, L A; Obrycki, J J

    2015-01-01

    Adults and nymphs of Empoasca fabae Harris (Hemiptera: Cicadellidae) and adults of predatory species in the families Coccinellidae, Anthocoridae, Nabidae, Chrysopidae, and Hemerobiidae were sampled in Iowa alfalfa fields from June to September in 1999 and 2000. The relationship between each predatory taxa and E. fabae was examined using regression analysis. In 2000, all predators were found to be positively correlated with the presence of E. fabae during all periods sampled and most likely contributed to mortality. Orius insidiosus (Say) (Hemiptera: Anthoridae) was the most numerous insect predatory species; population numbers ranged from 0 to 1 and 0.1 to 3.7 adults per 0.25 m(2) in 1999 and 2000, respectively. Partial life tables were constructed for E. fabae nymphs for two alfalfa-growing periods. Nymphs were grouped into three age intervals: first and second, third and fourth, and fifth instars. For the first alfalfa growing period examined, E. fabae nymphal mortality was 70% in 1999 and 49% in 2000. During the last growing period of each season (August-September), total nymphal mortality was relatively low (<25%). Adult E. fabae density ranged from 5.4 to 25.6 and 1.4-9.2 per 0.25 m(2) in 1999 and 2000, respectively. E. fabae population peaks were similar for each age interval in all growing periods. This study provides further information on the population dynamics of E. fabae and its relationship with select predatory species in Iowa alfalfa fields.

  10. Growth environment, harvest management and germplasm impacts on potential ethanol and crude protein yield in alfalfa

    Science.gov (United States)

    An alfalfa (Medicago sativa L) biomass energy production system would produce two products. Leaves would be separated from stems to produce a high protein feed for livestock and stems would be processed to produce ethanol. Therefore, maximum yields of both leaves and stems are essential for profitab...

  11. Crop mergers: Management of soil contamination and leaf loss in alfalfa

    Science.gov (United States)

    Maximizing the capacity and subsequent efficiency of the forage harvester necessitates consolidation (raking or merging) of alfalfa cuttings. Although rotary rakes are in wide use, the use of continuous pickup belt mergers is increasing in the Midwestern U.S. Previous work on crop consolidation is l...

  12. Draft Genome Sequence of Pseudomonas syringae pv. syringae ALF3 Isolated from Alfalfa.

    Science.gov (United States)

    Harrison, James; Dornbusch, Melinda R; Samac, Deborah; Studholme, David J

    2016-02-11

    We report here the annotated draft genome sequence of Pseudomonas syringae pv. syringae strain ALF3, isolated in Wyoming. A comparison of this genome sequence with those of closely related strains of P. syringae adapted to other hosts will facilitate research into interactions between this pathogen and alfalfa.

  13. Uptake of oxytetracycline and its phytotoxicity to alfalfa (Medicago sativa L.)

    Energy Technology Data Exchange (ETDEWEB)

    Kong, W.D. [Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China); Zhu, Y.G. [Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China)]. E-mail: ygzhu@rcees.ac.cn; Liang, Y.C. [Ministry of Agriculture Key Laboratory of Plant Nutrition and Nutrient Cycling, Institute of Soils and Fertilizers, Chinese Academy of Agricultural Sciences, Beijing 100081 (China); Zhang, J. [Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China); Smith, F.A. [Soil and Land Systems, School of Earth and Environmental Sciences, University of Adelaide, DP 636, Adelaide, SA 5005 (Australia); Yang, M. [Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085 (China)

    2007-05-15

    A series of experiments were conducted in a hydroponic system to investigate the uptake of oxytetracycline (OTC) and its toxicity to alfalfa (Medicago sativa L.). OTC inhibited alfalfa shoot and root growth by up to 61% and 85%, respectively. The kinetics of OTC uptake could be well described by Michaelis-Menten equation with V {sub max} of 2.25 {mu}mol g{sup -1} fresh weight h{sup -1}, and K {sub m} of 0.036 mM. The uptake of OTC by alfalfa was strongly inhibited by the metabolic inhibitor, 2,4-DNP (2,4-dinitrophenol), at pH 3.5 and 6.0, but not by the aquaporin competitors, glycerol and Ag{sup +}. OTC uptake, however, was significantly inhibited by Hg{sup 2+}, suggesting that the inhibition of influx was due to general cellular stress rather than the specific action of Hg{sup 2+} on aquaporins. Results from the present study suggested that OTC uptake into alfalfa is an energy-dependent process. - Plant uptake of antibiotic oxytetracycline is energy-dependent.

  14. Selective lignin downregulation leads to constitutive defense response expression in alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Gallego-Giraldo, Lina; Jikumaru, Yusuke; Kamiya, Yuji; Tang, Yuhong; Dixon, Richard A

    2011-05-01

    • Downregulation of hydroxycinnamoyl CoA: shikimate hydroxycinnamoyl transferase (HCT) in alfalfa (Medicago sativa) reduces lignin levels and improves forage quality and saccharification efficiency for bioethanol production. However, the plants have reduced stature. It was previously reported that HCT-down-regulated Arabidopsis have impaired auxin transport, but this has recently been disproved. • To address the basis for the phenotypes of lignin-modified alfalfa, we measured auxin transport, profiled a range of metabolites including flavonoids and hormones, and performed in depth transcriptome analyses. • Auxin transport is unaffected in HCT antisense alfalfa despite increased flavonoid biosynthesis. The plants show increased cytokinin and reduced auxin levels, and gibberellin levels and sensitivity are both reduced. Levels of salicylic, jasmonic and abscisic acids are elevated, associated with massive upregulation of pathogenesis and abiotic stress-related genes and enhanced tolerance to fungal infection and drought. • We suggest that HCT downregulated alfalfa plants exhibit constitutive activation of defense responses, triggered by release of bioactive cell wall fragments and production of hydrogen peroxide as a result of impaired secondary cell wall integrity.

  15. Incorporation of p-coumarates into the cell walls of alfalfa changes the lignin composition

    Science.gov (United States)

    In general, monocots can contain a significant amount of an ester-linked p-coumarate (pCA) in their cell walls, but its function is unclear. One hypothesis is that pCA aids in the formation of syringyl-rich regions during lignification. Alfalfa (Medicago sativa), a dicot, is a cultivated perennial f...

  16. Canopy visible and near-infrared reflectance data to estimate alfalfa nutritive attributes before harvest

    Science.gov (United States)

    Proximal sensing could help improve profit margins by timing the cutting or harvesting of alfalfa (Medicago sativa L.), in rapid assessment of nutritive values, such as total nitrogen (N), neutral detergent fiber (NDF), and acid detergent fiber (ADF) as well as nutritive quality indicators such as r...

  17. Best Phd thesis Prize: Statistical analysis of ALFALFA galaxies: insights in galaxy

    NARCIS (Netherlands)

    Papastergis, E.

    2013-01-01

    We use the rich dataset of local universe galaxies detected by the ALFALFA 21cm survey to study the statistical properties of gas-bearing galaxies. In particular, we measure the number density of galaxies as a function of their baryonic mass ("baryonic mass function") and rotational velocity ("veloc

  18. Enhancing forage yields and soil conservation by interseeding alfalfa into silage corn

    Science.gov (United States)

    Recent field studies have identified prohexadione-calcium (PHD) as an effective plant growth regulator for enhancing the establishment of alfalfa interseeded into corn as a dual-purpose cover and forage crop. Foliar applications of PHD on seedlings doubled or tripled stand survival of interseeded al...

  19. Pythium and Fusarium species causing seed rot and damping-off of alfalfa

    Science.gov (United States)

    Seed rot and damping-off is an important disease of alfalfa, severely affecting stand establishment when conditions favor the disease. This disease may have been overlooked as a cause of poor stand establishment and reduced vigor of adult plants. Globally, 15 Pythium species have been found to cause...

  20. Alfalfa contains substantial 9-hydroperoxide lyase activity and a 3Z:2E-enal isomerase

    NARCIS (Netherlands)

    Vliegenthart, J.F.G.; Noordermeer, M.A.; Veldink, G.A.

    1999-01-01

    Fatty acid hydroperoxides formed by lipoxygenase can be cleaved by hydroperoxide lyase resulting in the formation of short-chain aldehydes and omega-oxo acids. Plant hydroperoxide lyases use 13- or 9-hydroperoxy linoleic and linolenic acid as substrates. Alfalfa (Medicago sativa L.) has been reporte

  1. (Almost) Dark Galaxies in the ALFALFA Survey: HI-bearing Ultra-Diffuse Galaxies, and Beyond

    Science.gov (United States)

    Leisman, Luke; Haynes, Martha P.; Giovanelli, Riccardo; ALFALFA Almost Darks Team

    2017-01-01

    Scaling relations between HI and stars in galaxies suggest strong ties between their atomic gas content and star formation laws. The Arecibo Legacy Fast ALFA (ALFALFA) blind extragalactic HI survey is well positioned to locate very low surface brightness sources that lie off these relations, the most extreme of which may fall below optical detection limits. Thus, the ALFALFA (Almost) Darks Project has been investigating extreme outliers from these relations by studying the ~1% of ALFALFA sources without apparent stellar counterparts in major optical surveys. We have obtained deep HI and optical imaging of 25 of these candidate "dark" sources. We find that most "dark" sources are not extreme "(almost) dark" galaxies. A few are rare OH Megamasers, redshifted into the ALFALFA bandpass, and many are part of large galactic plumes, stretching as far as 600 kpc from their host galaxy. However, a small handful of sources appear to be galaxies with extreme stellar systems. We find multiple systems with HI mass to stellar mass ratios an order of magnitude larger than typical gas rich dwarfs. Further, we find an isolated population of HI-bearing "ultra diffuse" galaxies (UDGs), with stellar masses of dwarfs, but HI and optical radii of L* galaxies. We suggest that these sources may be related to recently reported gas poor, quiescent UDGs.

  2. Screening of Cd tolerant genotypes and isolation of metallothionein genes in alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Wang, Xiaojuan; Song, Yu; Ma, Yanhua; Zhuo, Renying; Jin, Liang

    2011-12-01

    In order to evaluate Cd tolerance in wide-ranging sources of alfalfa (Medicago sativa) and to identify Cd tolerant genotypes which may potentially be useful for restoring Cd-contaminated environments, thirty-six accessions of alfalfa were screened under hydroponic culture. Our results showed that the relative root growth rate varied from 0.48 to 1.0, which indicated that different alfalfa accessions had various responses to Cd stress. The candidate fragments derived from differentially expressed metallothionein (MT) genes were cloned from leaves of two Cd tolerant genotypes, YE and LZ. DNA sequence and the deduced protein sequence showed that MsMT2a and MsMT2b had high similarity to those in leguminous plants. DDRT-PCR analysis showed that MsMT2a expressed in both YE and LZ plants under control and Cd stress treatment, but MsMT2b only expressed under Cd stress treatment. This suggested that MsMT2a was universally expressed in leaves of alfalfa but expression of MsMT2b was Cadmium (Cd) inducible.

  3. Soil factors exhibit greater influence than bacterial inoculation on alfalfa growth and nitrogen fixation.

    Science.gov (United States)

    Neumann, Ute; Kosier, Bob; Jahnke, Joachim; Priefer, Ursula B; Al-Halbouni, Djamila

    2011-09-01

    In order to study the effects of soil factors and bacterial inoculation on alfalfa (Medicago sativa), plants were inoculated with Ensifer meliloti L33 and Azospirillum brasilense Sp7 in pot experiments using two different soils separately as well as in a mixture. One soil was contaminated with chemical waste products; the other was an arable soil. Soil factors, including the availability of macro- and micronutrients as well as carbon and nitrogen contents, were found to exhibit a much greater influence on the growth of alfalfa than any of the inoculations. In contaminated soil, the shoot and root growth of alfalfa was decreased and nodules were diminished and ineffective. Bacterial inoculations did not significantly improve this hostile growth environment. However, in a mixture (44% arable, 22% contaminated soil, 34% vermiculite), growth conditions for alfalfa were improved so that shoot dry weight and nodule numbers increased up to 100- and 20-fold, respectively, compared with the contaminated soil. For the strain L33, its persistence in the rhizosphere was correlated to the presence of its host plant, but its dynamics were influenced by competition with indigenous rhizobia. The strain Sp7, once provided with a suitable soil, was not dependent on the plant's rhizosphere, but it enhanced the performance of L33 and native rhizobia.

  4. Evaluation of subsurface drip irrigation design and management parameters for alfalfa

    NARCIS (Netherlands)

    Kandelous, M.M.; Kamai, T.; Vrugt, J.A.; Šimůnek, J.; Hanson, B.; Hopmans, J.W.

    2012-01-01

    Alfalfa is one of the most cultivated crops in the US, and is being used as livestock feed for the dairy, beef, and horse industries. About nine percent of that is grown in California, yet there is an increasing concern about the large amounts of irrigation water required to attain maximum yield. We

  5. Effects of wrapping time delays on the nutritive value of baled alfalfa silages

    Science.gov (United States)

    Baled silages are an attractive forage conservation option, especially for small and mid-sized beef and dairy producers. Our objectives were to test the effects of delayed wrapping on the nutritive value of baled alfalfa silages on a pre- and post-storage basis. A secondary objective was to evaluate...

  6. Milk production response to feeding alfalfa silage inoculated with Lactobacillus plantarum

    Science.gov (United States)

    In mini-silo trials, silages treated with a Lactobacillus plantarum silage inoculant (Ecosyl, Yorkshire, UK) had increased in vitro rumen microbial biomass production compared to untreated. Our objective was to determine if alfalfa silage treated with this inoculant could produce a milk production r...

  7. Managing foliar and root rot diseases of alfalfa for improving yield and persistance

    Science.gov (United States)

    Resistance to the six most common diseases across the United States is available in modern alfalfa cultivars. However, several diseases are becoming increasing problems in many parts of the country. Recognizing these problems is the first step in using crop management strategies to minimize diseases...

  8. Application of grain baits to control common vole Microtus arvalis (Pallas, 1778 in alfalfa crops, Serbia

    Directory of Open Access Journals (Sweden)

    Jokić G.

    2012-01-01

    Full Text Available In order to compare the efficacies of conventional (cholecalciferol and bromadiolone and new (sodium selenite rodenticides, applied in the grain bait formulation on the whole-grain of wheat (Triticum aestivum and triticale (Triticasecale in alfalfa crops, experiments were conducted at two sites near Belgrade, Serbia, in the spring of 2009, using a standard EPPO method. The presence of rodent populations, their spatial distribution and density indices were evaluated by pretreatment census and rodenticide efficacy by counting active holes, 14 and 28 days after treatment. The average Microtus arvalis numbers of 158/ha and 184/ha were found to cause 7.4% and 9.6% alfalfa green biomass yield decreases, respectively. Twenty-eight days after treatment, the average efficacy of grain bait formulation (on wheat and triticale grains of sodium selenite and cholecalciferol was 81%, while bromadiolone which had a higher efficiency, 85%, in the control of the common vole in alfalfa crops. The analysis of variance (ANOVA showed that the origin of active substances, bases and associated interactions a.s x based on the efficacy-investigated grain baits did not have a statistically significant impact on the expression efficiency of the tested baits. Triticale grains can be used as carriers of active substances, sodium selenite, cholecalciferol or bromadiolone in preparation baits. Control of M. arvalis with the new rodenticide, sodium selenite, gave efficacy results about equal to that of cholecalciferol and bromadiolone and, therefore, provided a possible alternative rodenticide for vole control in alfalfa.

  9. Emergence, forage production, and ion relations of alfalfa in response to saline waters

    Science.gov (United States)

    Alfalfa (Medicago sativa L) is an important crop utilized in regions under irrigation commonly impacted by salinity. In this study we evaluate the effect of salinity, as a continual process, from emergence to mature plant growth in successive harvests. We studied emergence, biomass production, salt ...

  10. First report of Alfalfa mosaic virus infecting basil (Ocimum basilicum L.) in California.

    Science.gov (United States)

    Basil (Ocimum basilicum L.) plants collected from a field in Imperial County, CA in May, 2011 were found to exhibit yellowing, chlorotic sectors and spots on leaves, resulting in plants being unmarketable. Total nucleic acid was extracted from plants and tested by RT-PCR for the presence of Alfalfa...

  11. Photosynthate partitioning and nitrogen fixation of alfalfa and birdsfoot trefoil. [Lotus corniculatus L. ; Medicago sativa L

    Energy Technology Data Exchange (ETDEWEB)

    Shieh, W.J.

    1985-01-01

    Nodule mass and number are usually correlated with rates of nitrogen fixation in legumes. Birdsfoot trefoil (Lotus corniculatus L.) with more than twice the nodule number and mass, however, fixes far less nitrogen than alfalfa (Medicago sativa L.) at the same age. In this research, photosynthesis and photosynthate partitioning and utilization in relation to nitrogen fixation of alfalfa and birdsfoot trefoil were examined in order to determine their relationship to nitrogen fixation potential. Photosynthate to nodules was studied using /sup 14/CO/sub 2/ labeling techniques. Partitioning patterns were altered by shading and dark depletion treatments. Efficiency of photosynthate utilization was examined by determining turnover of /sup 14/C photosynthate in nodule metabolites and by studying rates of cyanide-resistant and cyanide-sensitive O/sub 2/ uptake. Alfalfa nodule activity was greater than trefoil expressed on a hole pot or nodule dry weight basis. Both shading and dark treatments significantly reduced nodule activity as estimated by the acetylene reduction assay. Shoots of both species were found to be the dominant sinks for photosynthate. Percentage /sup 14/C recovered in alfalfa roots was more than twice that of trefoil at 1,2,3,4 and 24 h after labeling. Greater relative specific radioactivity (RSA) in nodules of both species suggests that they were stronger sinks for current photosynthate than roots.

  12. Variable salinity responses of 12 alfalfa genotypes and comparative expression analyses of salt-response genes

    Science.gov (United States)

    Twelve alfalfa genotypes that were selected for biomass under salinity, differences in Na and Cl concentrations in shoots and K/Na ratio were evaluated in this long-term salinity experiment. The selected plants were cloned to reduce genetic variability within each genotype. Salt tolerance (ST) index...

  13. Evaluation of alfalfa (Medicago sativa L.) populations' response to salinity stress

    Science.gov (United States)

    Alfalfa is a moderately salt tolerant crop with high economic return, therefore more suitable for production with lower quality water than most high value crops. This study was conducted to examine the effects of the irrigation water salt type (ST=Cl- or SO4 2-) and five salinity levels (ECiw= 0.85,...

  14. Identification of Molecular Marker Linked to Salt Tolerance Gene in Alfalfa

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    The study has established the F2 offspring obtained by crossing salt-tolerant with salt-sensitive alfalfa, and appraised the salt-tolerant F2 offspring seedling was evaluated in pot culture. With the F2 segregated population, the research has obtained a molecular marker linked with salt-tolerant genes of alfalfa using the improved BSA combined with RAPD. The RAPD PCR products were excised from the agarose gel and purified using a kit, then were mixed with pMD-18T vector and sequenced. Sequencing result indicated the RAPD marker was 1 438 bp in length. Similarity researches using blast in Genbank indicated that the nucleotide sequence of the RAPD marker showed 93% and 91% similarity with mth2-6el8 gene fragment (347 bp) and mth2-33122 gene fragment (334 bp) of Medicago truncatula respectively. Medicago truncatula is a close relative of alfalfa and Mth2-6e18 is a molecular marker of the gene coding for a cysteine protease which was salt inducible in some plants. These results indicated the RAPD marker was possibly related to cysteine protease genes in alfalfa.

  15. Correlation of fermentation characteristics with intake and digestibility of alfalfa silage in gestating ewes

    Science.gov (United States)

    Baled silage production provides benefits to farmers because it reduces leaf losses, and requires a shorter wilting time, thereby limiting risks of exposure to rain compared with making hay. Our objective was to investigate the correlation of alfalfa silage fermentation parameters with intake and di...

  16. Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)

    Science.gov (United States)

    Activities of peroxidase (POD) and superoxide dismutase (SOD) enzymes were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-Ias) or hygromycin phosphotransferase (...

  17. Morphological development of Alfagraze cultivar and Crioula alfalfa populations selected at the seedling stage Desenvolvimento morfológico do cultivar Alfagraze e de populações de alfafa Crioula selecionadas em estádio de plântula

    Directory of Open Access Journals (Sweden)

    Daniela Favero

    2009-04-01

    Full Text Available The objective of this study was to compare two populations of Crioula alfalfa cultivars, selected by the length of the seedling second internode (Crioula-LI, long internode = 2.5 cm; Crioula-SI, short internode = 1.5 cm with Alfagraze cultivar in order to verify the seedling morphology value for the selection of more prostrate plants. The plants were grown individually in pots in a semi-protected environment and harvested at 74, 104, 134, 164, 194 and 224 days. Crioula populations varied in terms of height, leaf area, root volume, dry matter yield, and the Crioula-LI population outnumbered that of Crioula-SI. For height, length of internode and primary stem, Alfagraze (45.1 cm, 2.1 cm and 16.7 cm was similar to Crioula-SI (37.6 cm, 2.3 cm and 18.2 cm; however, the leaf area (2.028 cm² and dry matter yield (20.3 g did not differ from those of Crioula-LI (2.021 cm², 21 g. The Alfagraze cultivar surpassed the Crioula population in terms of procumbency, crown diameter and crown stem number. At the flowering stage, the Crioula-LI cultivar had total stem number (61/plant higher than Alfagraze (47/plant and Crioula-SI (41/plant cultivars. The length of the seedling internode is a promising marker for the selection of alfalfa, being a predictive factor for plant height, stem number, root volume and leaf area, and can be useful in the alfalfa breeding.O objetivo neste trabalho foi comparar duas populações de alfafa cv. Crioula, selecionadas pelo comprimento do segundo entrenó da plântula (Crioula-EL, entrenó longo = 2,5 cm; Crioula-EC, entrenó curto = 1,5 cm, e o cultivar Alfagraze, com a finalidade de verificar o valor da morfologia da plântula para seleção de plantas mais prostradas. As plantas foram cultivadas individualmente em vasos, em ambiente semiprotegido, e colhidas aos 74, 104, 134, 164, 194 e 224 dias de idade. As populações de Crioula variaram entre si quanto à altura, à área foliar, ao volume de raízes e à produção de

  18. Assessment of wheat variety distinctness using SSR markers

    Institute of Scientific and Technical Information of China (English)

    WANG Li-xin; QIU Jun; CHANG Li-fang; LIU Li-hua; LI Hong-bo; PANG Bin-shuang; ZHAO Chang-ping

    2015-01-01

    Assessment of variety distinctness is important for both the registration and the protection of particular variety. However, the current testing system, which assesses a range of morphological characters of each pair of varieties grown side-by-side, is time-consuming and is not suitable for the assessment of hundreds of samples. The objective of this study was to develop a procedure for the assessment of wheat variety distinctness using simple sequence repeat (SSR) markers. A comparison between the molecular and morphological proifle of 797 varieties was made. On the basis of the comparison, pairs of va-rieties with a genetic similarity value (GSV) ≤90% were deemed to be distinct, accounting for ~85% of varieties assessed in wheat regional trials. For the remaining ~15% of varieties, GSVs between different varieties were >90%, among which ~35% were not distinct and the other ~65% were distinct. Therefore, if given a GSV>90%, the pairs of varieties should be morphologicaly assessed in the ifeld. To avoid any errors in the assessments, we proposed the elimination of contaminant plants from the sample before comparing the varietal genotypes, scoring of the genotype at each locus with a pair of alele numbers when constructing a molecular proifle, and faithfuly recording two aleles at a non-homozygous locus. To reduce the workload and cost, a three-grade markers comparison among varieties is suggested. In addition, 80 SSR markers and a technical procedure for assessment of wheat variety distinctness have been proposed. Based on the procedure, the distinctness assessment of ~85% of al wheat varieties is completed in our laboratory annualy. Consequently, total ifeld assessment has been reduced considerably.

  19. [Natural nucleotide polymorphism of the Srlk gene that determines salt stress tolerance in alfalfa (Medicago sativa L)].

    Science.gov (United States)

    Vishnevskaia, M S; Pavlov, A V; Dziubenko, E A; Dziubenko, N I; Potokina, E K

    2014-04-01

    Based on legume genome syntheny, the nucleotide sequence of Srlk gene, key role of which in response to salt stress was demonstrated for the model species Medicago truncatula, was identified in the major forage and siderate crop alfalfa (Medicago sativa). In twelve alfalfa samples originating from regions with contrasting growing conditions, 19 SNPs were revealed in the Srlk gene. For two nonsynonymous SNPs, molecular markers were designed that could be further used to analyze the association between Srlk gene nucleotide polymorphism and the variability in salt stress tolerance among alfalfa cultivars.

  20. Co-transforming bar and CsALDH genes enhanced resistance to herbicide and drought and salt stress in transgenic alfalfa (Medicago sativa L.

    Directory of Open Access Journals (Sweden)

    Zhen eDuan

    2015-12-01

    Full Text Available Drought and high salinity are two major abiotic factors that restrict the productivity of alfalfa. By application of the Agrobacterium-mediated transformation method, an oxidative responsive gene, CsALDH12A1, from the desert grass Cleistogenes songorica together with the bar gene associated with herbicide resistance, were co-transformed into alfalfa (Medicago sativa L.. From the all 90 transformants, 16 were positive as screened by spraying 1 mL L-1 10% Basta solution and molecularly diagnosis using PCR. Real-time PCR analysis indicated that drought and salt stress induced high CsALDH expression in the leaves of the transgenic plants. The CsALDH expression levels under drought (15 d and salt stress (200 mM NaCl were 6.11 and 6.87 times higher than in the control plants, respectively. In comparison to the WT plants, no abnormal phenotypes were observed among the transgenic plants, which showed significant enhancement of tolerance to 15 d of drought and 10 d of salinity treatment. Evaluation of the physiological and biochemical indices during drought and salt stress of the transgenic plants revealed relatively lower Na+ content and higher K+ content in the leaves relative to the WT plants, a reduction of toxic on effects and maintenance of osmotic adjustment. In addition, the transgenic plants could maintain a higher relative water content (RWC level, higher shoot biomass, fewer changes in the photosystem, decreased membrane injury, and a lower level of osmotic stress. These results indicate that the co-expression of the introduced bar and CsALDH genes enhanced the herbicide, drought and salt tolerance of alfalfa and therefore can potentially be used as a novel genetic resource for the future breeding programs to develop new cultivars.

  1. Weed control in dormant alfalfa (Medicago sativa L. with active ingredients’ metribuzin, imazetapyr and pronamide

    Directory of Open Access Journals (Sweden)

    Zvonko Pacanoski

    2017-03-01

    Full Text Available Field trials were conducted during 2008 – 2010 to evaluate weed control in dormant alfalfa (Medicago sativa L. with metribuzin, imazetapyr and pronamide. The weed population in all experimental years was consisted mainly of annual winter and spring grass and broadleaf weeds, and some perennial weeds. The number of weed species and weed density increased with the years of alfalfa growing, from second to the fourth year. Weed density in the untreated control plots was 201.0, 217.2 and 240.5 plants per m2 in 2008, 2009 and 2010, respectively. The most dominant weeds were Anthemis cotula, Capsell bursa-pastoris and Taraxacum officinale in 2008, Alopecurus myosuroides and Poa pratensis in 2009 and Millium vernale and Arabidopsis thaliana in 2010. Efficacy of herbicides in control of weeds was ranged of 91.8% (pronamide to 98.4% (metribuzin 1.0 kg*ha-1 in 2008, 93.1% (imazetapyr to 97.3% (metribuzin 1.0 kg*ha-1 in 2009 and 92.1% (imazetapyr to 97.3% (metribuzin 1.0 kg*ha-1 in 2010, respectively. Efficacy of herbicides in control of prevailing weeds during the 3 years field trial period was ranged of 48.5% to 100.0%. No visual alfalfa injured was determined by any rates during the experimental period, and consequently, none of the applied herbicides reduced first-harvest alfalfa yields. Alfalfa yield was markedly affected by herbicide efficacy in all experimental years, particularly in the second year, where yields of herbicide treatments were similar to that of the weed free control.

  2. Effectiveness of calcium hypochlorite on viral and bacterial contamination of alfalfa seeds.

    Science.gov (United States)

    Wang, Qing; Kniel, Kalmia E

    2014-10-01

    Alfalfa sprouts have been involved in numerous foodborne outbreaks, which has increased the awareness for seed and sprout safety. This study compared the effectiveness of calcium hypochlorite (Ca(OCl)2) on the inactivation of bacteria and viruses on alfalfa seeds and in the presence of a simulated organic load. Alfalfa seeds were inoculated with human norovirus (huNoV) genogroup II (GII), murine norovirus (MNV), Tulane virus (TV), Escherichia coli O104:H4, and Salmonella enterica serovar Agona. Seeds were treated with Ca(OCl)2 (2000 ppm or 20,000 ppm with the average of free chlorine 1388 ± 117 mg/L and 11,472 ± 1500 mg/L, respectively, pH adjusted to 7.00). The reduction of huNoV genomic copies indicated that huNoV was relatively resistant to Ca(OCl)2 regardless of concentrations. Significant reductions were observed in the order of TV OCl)2. A similar trend was found at 2000 ppm Ca(OCl)2 in the order of TV, Salmonella Agona, MNV OCl)2 at 20,000 ppm was more effective than 2000 ppm for all the organisms tested. This trend was also observed in samples containing an artificial organic material load. Ca(OCl)2 activity on virus inactivation decreased as the organic load increased. Reduction was greater in fetal bovine serum-containing samples compared to alfalfa seeds, indicating a close relationship between the organisms and alfalfa seeds. Ca(OCl)2 could not completely inactivate bacteria or viruses inoculated on seeds, and high levels of E. coli O104:H4 and Salmonella Agona were present on sprouts from sanitized seed samples following a 7-day germination period.

  3. Evaluation of energy input and greenhouse gases emissions from alfalfa production in the Sistan region, Iran

    Directory of Open Access Journals (Sweden)

    Mohammad Reza Asgharipour

    2016-11-01

    Full Text Available The recognition of forage production methods that maximize energy efficiency and minimize Greenhouse Gases (GHGs emissions is essential. The aims of this survey were to assess the energy consumption, emissions of GHGs and global warming potential (GWP of alfalfa production systems in Sistan region, Sistan and Baluchestan province in the South–east of Iran. Data were collected randomly from 110 alfalfa farm using face-to-face questionnaire survey. Energy inputs included chemical fertilizers, diesel fuel, pesticides, seed, machinery and human labor. The results indicated that average total input and output energies in alfalfa production during the entire lifetime of the farm were 313.52GJha−1 and 962.85GJha−1, respectively. The most important energy inputs belonged to electricity (72.5%, followed by diesel fuel (12.3% and N fertilizer (6.0%. Energy use efficiency and energy productivity were 3.07 and 0.209kgMJ−1, respectively. Share of direct and indirect energy were 85% and 15%, respectively. Total emissions of CO2, N2O and CH4 in alfalfa farms were 8262.67kgha−1, 557.31kgha−1 and 7.65kgha−1, respectively. Hence, total GWP was 181190 kg CO2eha−1 and 2.77 kg of CO2ekg−1 of dry hay produced. In terms of CO2e, 95.3% of the GWP originate from N2O, 4.6% from CO2 and 0.1% from CH4. Accordingly, efficient use of energy is essential to reduce the greenhouse gas emissions and environmental impact in alfalfa agroecosystems.

  4. Digestibility and rate of passage by lambs of water-stressed alfalfa.

    Science.gov (United States)

    Undersander, D J; Cole, N A; Naylor, C H

    1987-06-01

    Two lamb digestion experiments were conducted to evaluate the effect of alfalfa [Medicago sativa (L.)] grown under varying levels of water deficiency (stress) on the rate of passage and digestibility of various fibrous components. Experiment 1 consisted of a randomized complete block design in which 12 Suffolk X Hampshire crossbred wethers averaging 40 kg were fed alfalfa hay grown at three (10, 15 or 20 cm water/ha) levels of water per harvest. Experiment 2 consisted of a switchback design in which four Hampshire wethers averaging 45 kg were fed alfalfa hay grown at two (5 or 20 cm/ha) levels of water per harvest. Forage yields ranged from 1,420 (10 cm/ha in Exp. 1) to 4,200 (20 cm/ha in Exp. 2) kg/ha. In both experiments, water stress reduced cell wall constituents (neutral detergent fiber), acid detergent fiber, lignin and cellulose content of the alfalfa hay. Organic matter digestibility was decreased when the percentage of leaves fell below 60% at the highest yield. Digestibility of N and the rate of NDF digestibility were not affected by water stress. The second experiment additionally included nutrient balance and rate of passage measurements. Greater (P less than .10) amounts of N and P were absorbed from water-stressed than nonstressed hay. Ruminal retention time of particulate markers tended (P less than .10) to increase with greater water stress. The results of this study are interpreted to indicate that while moderate water stress may have little effect on in vivo digestibility of alfalfa, severe stress may reduce digestibility of fibrous fractions and total organic matter.

  5. 添加初花期骆驼刺改善苜蓿青贮品质%Improve alfalfa silage quality by adding alhagi sparsifolia shap in initial bloom stage

    Institute of Scientific and Technical Information of China (English)

    蒋慧; 方雷; 周小玲; 李胜利

    2014-01-01

    The objectives of this work were to evaluate the effects of mixed silage of Alhagi sparsifolia Shap in initial bloom stage and alfalfa on silage quality, investigate a way of the silage of juicy alfalfa without additive, and provide a theoretical basis for the development and utilization of A. sparsifolia Shap. 100%of alfalfa (Ⅰ, as a control group) , 100%of A. sparsifolia Shap in initial bloom stage (Ⅱ) , or mixtures of their different percentages (Ⅲ:30%of A. sparsifolia Shap+70%of alfalfa,Ⅳ:50%of A. sparsifolia Shap+50%of alfalfa, Ⅴ:70%of A. sparsifolia Shap+30%of alfalfa) were used in this experiment and each treatment had three replicates. After 90 d of ensiling, silages were sampled, and sensory quality and fermented products (pH, lactic acid, acetic acid, propionic acid, butyric acid, isobutyric acid, isovaleric, methanol, ethanol, propanol and ammonia nitrogen) were evaluated. Chemical components (dry matter, crude protein, water soluble carbohydrate, neutral detergent fiber, acid detergent fiber), lactobacillus and yeast living cells were determinated for both fresh forages and silages. The results showed that the contents of dry matter (DM), water soluble carbohydrate (WSC) in mixed forages (as raw material to make silage) were higher than in alfalfa (P<0.05);the maximum of DM content was 26.48%in mixed forages and the minimum was 19.52%in alfalfa;and the maximum of WSC content was 6.27%in mixed forages and the minimum was 4.60%in alfalfa. The numbers of lactic acid bacteria living cells in mixed forages were higher than in alfalfa (P<0.05);the maximum in mixed forages was 7.2×105 (Ⅴ), and the minimum was 3.3×104 in alfalfa (Ⅰ). The quantities of yeast living cells in mixed forages were lower than in alfalfa (P<0.05); the maximum was 5.0×1011 in alfalfa (Ⅰ) , and the minimum of yeast living cells was 2.5×1010 in mixed forages (Ⅲ). After 90 d of ensiling, the lactobacillus living cells in silage was more than in forage at harvest (P<0

  6. Dematerialization: Variety, caution, and persistence.

    Science.gov (United States)

    Ausubel, Jesse H; Waggoner, Paul E

    2008-09-02

    Dematerialization, represented by declining consumption per GDP of energy or of goods, offers some hope for rising environmental quality with development. The declining proportion of income spent on staples as affluence grows, which income elasticity energy use and carbon emission to food consumption and fertilizer use, globally and in countries ranging from the United States and France to China, India, Brazil, and Indonesia. Because dematerialization and intensity of impact are ratios of parameters that may be variously defined and are sometimes difficult to estimate, their fluctuations must be interpreted cautiously. Nevertheless, substantial declining intensity of impact, and especially, dematerialization persisted between 1980 and 2006.

  7. Korean Adults' Attitudes towards Varieties of English

    OpenAIRE

    Kim, Young Soo

    2007-01-01

    This study investigates 43 Korean adults’ attitudes towards native and non-native varieties of English in relation to the perspective of EIL (English as an international language). This study addresses three research questions: 1) do Korean adults prefer certain varieties of English? 2) do Korean adults at least accept non-native varieties of English?, and 3) are Korean adults aware of different varieties of English? In order to examine participants’ language attitudes, this study employed bo...

  8. Integrative analysis of transgenic alfalfa (Medicago sativa L. suggests new metabolic control mechanisms for monolignol biosynthesis.

    Directory of Open Access Journals (Sweden)

    Yun Lee

    2011-05-01

    Full Text Available The entanglement of lignin polymers with cellulose and hemicellulose in plant cell walls is a major biological barrier to the economically viable production of biofuels from woody biomass. Recent efforts of reducing this recalcitrance with transgenic techniques have been showing promise for ameliorating or even obviating the need for costly pretreatments that are otherwise required to remove lignin from cellulose and hemicelluloses. At the same time, genetic manipulations of lignin biosynthetic enzymes have sometimes yielded unforeseen consequences on lignin composition, thus raising the question of whether the current understanding of the pathway is indeed correct. To address this question systemically, we developed and applied a novel modeling approach that, instead of analyzing the pathway within a single target context, permits a comprehensive, simultaneous investigation of different datasets in wild type and transgenic plants. Specifically, the proposed approach combines static flux-based analysis with a Monte Carlo simulation in which very many randomly chosen sets of parameter values are evaluated against kinetic models of lignin biosynthesis in different stem internodes of wild type and lignin-modified alfalfa plants. In addition to four new postulates that address the reversibility of some key reactions, the modeling effort led to two novel postulates regarding the control of the lignin biosynthetic pathway. The first posits functionally independent pathways toward the synthesis of different lignin monomers, while the second postulate proposes a novel feedforward regulatory mechanism. Subsequent laboratory experiments have identified the signaling molecule salicylic acid as a potential mediator of the postulated control mechanism. Overall, the results demonstrate that mathematical modeling can be a valuable complement to conventional transgenic approaches and that it can provide biological insights that are otherwise difficult to obtain.

  9. Measuring residual feed intake in dairy heifers fed an alfalfa (Medicago sativa) cube diet.

    Science.gov (United States)

    Waghorn, G C; Macdonald, K A; Williams, Y; Davis, S R; Spelman, R J

    2012-03-01

    Selection for divergence between individuals for efficiency of feed utilization (residual feed intake, RFI) has widespread application in the beef industry and is usually undertaken when animals are fed diets based on silages with grain. The objective of this research was to develop a feeding system (using Gallagher, Hamilton, New Zealand, electronics) to measure RFI for growth in Holstein-Friesian heifers (aged 5-9 mo), and identify divergent individuals to be tested for RFI during lactation. A dry forage diet (alfalfa cubes) was fed because intakes could be measured accurately, and the New Zealand dairy industry (4.4 million milking cows in lactation) relies heavily on forage feeding. The evaluation was undertaken over 3 yr with 1,052 animals fed in a facility for 7 wk, and weighed 3 times weekly. The mean age at the start of measurements was 215 d, body weight (BW) 189 kg, and mean daily dry matter intakes averaged 6.7 kg. Body weight gain (all animals) averaged 0.88 kg/d. The RFI was determined as the residuals from the regression of mean intake on mean BW(0.75) and daily BW gain of individuals. Actual and fitted intakes were strongly related (R(2) = 0.82). In terms of gross efficiency (feed intake/BW gain), RFI+year explained 43% of the variation, BW gain+year explained 66%, and RFI+BW gain+year explained 79% of the variation (all Pdry matter/d. The most and least efficient animals will be tested for RFI during lactation and genetic markers will be identified for the trait.

  10. Maize variety and method of production

    Energy Technology Data Exchange (ETDEWEB)

    Pauly, Markus; Hake, Sarah; Kraemer, Florian J

    2014-05-27

    The disclosure relates to a maize plant, seed, variety, and hybrid. More specifically, the disclosure relates to a maize plant containing a Cal-1 allele, whose expression results in increased cell wall-derived glucan content in the maize plant. The disclosure also relates to crossing inbreds, varieties, and hybrids containing the Cal-1 allele to produce novel types and varieties of maize plants.

  11. On Spinor Varieties and Their Secants

    Directory of Open Access Journals (Sweden)

    Laurent Manivel

    2009-07-01

    Full Text Available We study the secant variety of the spinor variety, focusing on its equations of degree three and four. We show that in type Dn, cubic equations exist if and only if n ≥ 9. In general the ideal has generators in degrees at least three and four. Finally we observe that the other Freudenthal varieties exhibit strikingly similar behaviors.

  12. On varieties with higher osculating defect

    CERN Document Server

    De Poi, Pietro; Ilardi, Giovanna

    2012-01-01

    In this paper, using the method of moving frames, we generalise some of Terracini's results on varieties with tangent defect. In particular, we characterise varieties with higher order osculating defect in terms of Jacobians of higher fundamental forms and moreover we characterise varieties with "small" higher fundamental forms as contained in scrolls.

  13. Effects of Herbivore Stress by Acyrthosiphon pisum on the Contents of Tannin and Physiological Activity in Different Alfalfa Cultivars%豌豆蚜刺吸胁迫对不同苜蓿品种体内单宁含量及生理活性的影响

    Institute of Scientific and Technical Information of China (English)

    武德功; 王森山; 刘长仲; 胡桂馨; 杜军利; 贺春贵

    2011-01-01

    It is necessary to study the relationship between aphid stress and physiological activity in different alfalfa yarieties for breeding of resistant alfalfa varieties and sustainable control of aphids. The dynamics of Malondialdehyde (MDA) and Tannin contents and the activities of protective enzymes (Superoxide dismutase (SOD), Peroxidase (POD)) were studied in the leaves of four alfalfa cultivars, Gannong No. 5 (G5), Gannong No. 3 (G3), Gloden Empress (JH), and Hunter River (Hu), infested by pea aphid,Acyrthosiphon pisum Harris. Results show that the MDA contents of resistant variety (G5) were lower than that of the susceptible variety (Hu) and low resistant varieties (G3) when their leaves were piercingsucked by the aphid. The Tannin contents of resistant variety (G5) and the susceptible variety (Hu) were lower than that of the low resistant ones (G3 and JH), While the SOD and POD activities in the susceptible variety (Hu) appeared lower than that of the resistant variety (G5) and low resistant varieties (G3,JH). Throughout the period of aphid attacks, the activities of SOD and POD of all varieties rose initially then declined, whereas the dynamics of MDA and Tannin contents of four alfalfa cultivars were irregular.Therefore, under the aphid stress, the contents of MDA and the activity of SOD, POD in susceptible, low resistant and resistant varieties are closely related to the aphid-resistance of alfalfa. These biochemical markers may be used as physiological indices for aphid-resistance appraisal. However, the relationship between Tannin contents and aphid-resistance of alfalfa requires further research.%通过对甘农5号(G5)、甘农3号(G3)、金皇后(JH)和猎人河(Hu)4种不同抗蚜苜蓿(Medicago sativa L.)品种受豌豆蚜(Acyrthosiphon pisum)胁迫后其叶片内丙二醛(MDA)和单宁含量及防御性酶(超氧化物歧化酶(SOD)和过氧化物酶(POD))活性的动态变化研究,以期明确各项生理指标与抗虫性之间的关系.结果表

  14. Effects of Fractionation Methods on the Isolation of Fiber-rich Cake from Alfalfa and Ethanol Production from the Cake

    Directory of Open Access Journals (Sweden)

    Shuangning Xiu

    2014-04-01

    Full Text Available Freshly harvested alfalfa was fractionated using centrifugation and filtration, whereby alfalfa was separated into a fiber-rich cake and a nutrient-rich juice. The solid cakes from the above separation processes were used as the feedstock for ethanol production using separate hydrolysis and fermentation. The filtration process proved to be more efficient at reducing the solids mass transfer to the juice than the centrifuge process. Glucose from filtered alfalfa solid cake can be efficiently fermented to ethanol with 75% of the theoretical yield. In conclusion, centrifugation was not as effective as filtration in removing particulates and colloidal matter from alfalfa. The filtration process resulted in a solid cake with a higher cellulose digestibility, which leads to a higher ethanol production.

  15. Ten New Crop Varieties(Lines) Released Recently in HAAS

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    @@Two-line Hybrid Glutinous Sorghum Xiangliangyou Nuoliang 1 It is the first two-line hybrid glutinous sorghum variety with good quality and high yield in the world developed by the Sorghum Research & Development Center,HAAS. It was appraised to release by Hunan Provincial Crop Variety Release Committee (HPCVRC) in 1996,and listed as an important achievement being popularized in the"Ninth Five-Year Plan" period in China.

  16. A Characterization of P-Compatible Varieties

    Institute of Scientific and Technical Information of China (English)

    Ivan Chajda; Klaus Denecke; Shelly L. Wismath

    2007-01-01

    P-Compatibility is a hereditary property of identities which generalizes the properties of normality and externality of identitiesChajda characterized the normalization of a variety by an algebraic construction called a choice algebraIn this paper, we generalize this characterization to the least P-compatible variety P(V) determined by a variety V for any partition P using P-choice algebrasWe also study the clone of (strongly) P-compatible n-ary terms of a variety V, and relate identities of this clone to (strongly) P-compatible hyperidentities of the variety V.

  17. THE EFFECT OF PRIMING ON GERMINATION AND SEEDLING GROWTH OF ALFALFA (MEDICAGO SATIVA L.) UNDER SALINITY STRESS

    OpenAIRE

    Jorjandi M.; SHARIFI SIRCHI G.R.

    2012-01-01

    Salinity stress is an abiotic stress which has harmful effects on germination, growth and yield of alfalfa (Medicago sativa L.) in many parts of the world. Seed priming is a way of increasing salt tolerance of plants. In this study, the effect of seed priming on germination and growth of alfalfa seedlings, Bami cultivar, under salinity condition was investigated. According to germination percentage of seeds under In vitro conditions, salicylic acid, Vitamin B12 and distilled water were select...

  18. Effect of Arbuscular Mycorrhizal Fungi Glomus spp. Inoculation on Alfalfa Growth in Soils with Copper Efecto de la Inoculación con Hongos Micorrízicos Arbusculares Glomus spp. sobre el Crecimiento de Alfalfa en Suelos con Cobre

    Directory of Open Access Journals (Sweden)

    Daniela Novoa M

    2010-06-01

    Full Text Available Soils near mining centers usually have high heavy metal (HM levels. It has been found that some plants associated with arbuscular mycorrhizal fungi (AMF improve growth and tolerance to HM in soils. This symbiosis is a biological resource for degraded soil recovery. The objective of this study was to determine the effect of inoculating AMF (Glomus spp. on alfalfa (Medicago sativa L. growth in agricultural soils with different copper (Cu levels for degraded soil recovery. To this effect, alfalfa seeds were grown in soils from the Catemu and Casablanca valleys and inoculated with AMF. Plant height, stem diameter, and number of leaves were measured weekly. Dry matter, mycorrhizal colonization, and Cu concentration in alfalfa plant tissues were measured after 81 days. Inoculation increased plant height by 24%, stem diameter by 11%, and number of leaves by 34%. Inoculation had a significant effect (p ≤ 0.05 on alfalfa plants that were grown in soil with the highest Cu concentration, but had no effect on Cu accumulation in alfalfa plant tissues. A direct relationship was observed between Cu accumulation in alfalfa and Cu concentration in soils. It was concluded that alfalfa inoculated with Glomus spp. is applicable to the soil recovery process whenever soil properties can ensure inoculum effectiveness on alfalfa growth, and avoid toxicity by excessive Cu in alfalfa plant tissues.Los suelos cercanos a centros de actividad minera suelen presentar altos niveles de metales pesados (HM. Se ha encontrado que algunas plantas asociadas a hongos micorrízicos arbusculares (AMF mejoran su crecimiento y tolerancia a los HM presentes en los suelos. Esta simbiosis constituye un recurso biológico para la recuperación de suelos degradados. El objetivo de este estudio fue determinar el efecto de la inoculación con AMF (Glomus spp. sobre el crecimiento de alfalfa (Medicago sativa L. en suelos agrícolas con distintos niveles de cobre (Cu para la recuperación de

  19. Lifting, restricting and sifting integral points on affine homogeneous varieties

    CERN Document Server

    Gorodnik, Alexander

    2010-01-01

    In a previous paper {GN2} an effective solution of the lattice point counting problem in general domains in semisimple S-algebraic groups and affine symmetric varieties was established. The method relies on the mean ergodic theorem for the action of G on G/Gamma, and implies uniformity in counting over families of lattice subgroups admitting a uniform spectral gap. In the present paper we extend some methods developed in {NS} and use them to establish several useful consequences of this property, including : Effective upper bounds on lifting for solutions of congruences in affine homogeneous varieties, effective upper bounds on the number of integral points on general subvarieties of semisimple group varieties, effective lower bounds on the number of almost prime points on symmetric varieties, and effective upper bounds on almost prime solutions of Linnik-type congruence problems in homogeneous varieties.

  20. Producing ethanol from alfalfa stems with an acid ensilage pretreatment

    Science.gov (United States)

    Lignocellulosic biomass offers the potential to provide sustainable sugar streams from a variety of materials, including agricultural and forest residuals, and high-yielding bioenergy crops. However, if cropland is diverted from feed production to production of energy crops, there will still be an i...

  1. Cryopreservation of Bituminaria bituminosa varieties and hybrids.

    Science.gov (United States)

    Gisbert, Carmina; Dabauza, Mercedes; Correal, Enrique; Swennen, Rony; Panis, Bart

    2015-10-01

    Bituminaria bituminosa (L.) C.H. Stirton is a drought tolerant, perennial legume pasture species and a source of pharmaceutical compounds. Bituminaria breeding programs aim to develop and conserve hybrids with desirable traits such as high forage quality, tolerance to biotic or abiotic stresses, and high contents of furanocoumarins. In this work we present a cryopreservation study of different B. bituminosa accessions: two varieties and eight intervarietal hybrids resulting from crosses between the three botanical varieties: var. bituminosa, var. crassiuscula, and var. albomarginata. No previous work on cryopreservation of Bituminaria species has been reported. We applied the ultra-fast cooling method, using droplet vitrification on aluminum foil strips. First, we investigated the PVS2 toxicity and cryopreservation damage in two genotypes, comparing three PVS2 treatments and two culture media. An incubation of 30 min in PVS2 resulted in regeneration rates after cryopreservation higher than 80%. The MS medium was selected for optimal meristem outgrowth, in order to avoid the prominent callus formation that was observed in the presence of BAP. These conditions were subsequently used to cryopreserve eight other genotypes. The results were highly variable; 45 days after cryopreservation, survival ranged between 22% and 98% while regeneration ranged between 0% and 96%, depending on the accession. A significant and positive correlation was observed between survival and regeneration. At 90 days post culture plantlets could be recovered from cryopreserved explants of all genotypes. This study shows that the droplet vitrification method is promising for the cryopreservation of eight of the 10 genotypes assayed and the method can thus be applied to develop a cryobank of B. bituminosa.

  2. The effects of aqueous extract of alfalfa on blood glucose and lipids in alloxan-induced diabetic rats

    Science.gov (United States)

    Amraie, Esmaiel; Farsani, Masome Khosravi; Sadeghi, Leila; Khan, Tayaba Naim; Adavi, Zohrab

    2015-01-01

    Diabetes is a common metabolic disorder that is specified by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. The use of nonpharmacological treatments (herbal agents) is a new approach in the management of diabetes. The aim of this study was to investigate the effect of aqueous extract of alfalfa on blood glucose and serum lipids in alloxan-induced diabetic rats. In this study, 32 female rats (210–250 g) were used which were divided randomly into 4 groups including intact control group, diabetic control group, and 2 diabetic groups which received 250 and 500 mg/kg doses of aqueous extract of alfalfa, respectively. In the diabetic groups, alloxan-monohydrate was injected peritoneally to create diabetic condition. The two last groups orally received aqueous extract of alfalfa for 21 days. At the end of experiment, sugar, cholesterol, triglycerides, high-density and low-density lipoprotein, and aspartate aminotransferase (ALT) and alanine aminotransferase (AST) levels were measured in the samples. Consumption of aqueous alfalfa extract significantly reduced glucose, cholesterol, triglycerides, and low-density lipoprotein (LDL) levels in the diabetic rats but enhanced high-density lipoprotein (HDL) levels. ALT and AST liver enzyme levels were also reduced in blood. Histological examination showed that the aqueous alfalfa extract caused reconstruction of damaged liver and enhanced Langerhans islets’ diameter in pancreas. Therefore, all signs of diabetes were improved by oral administration of alfalfa in defined dose. PMID:26525173

  3. Alfalfa nutritive quality for ruminant livestock as influenced by ambient air quality in west-central Alberta

    Energy Technology Data Exchange (ETDEWEB)

    Lin, J.C. [Department of Animal Sciences, Auburn University, Auburn, AL 36849 (United States); Nosal, M. [Department of Mathematics and Statistics, University of Calgary, Calgary, AB T2N 1N4 (Canada); Muntifering, R.B. [Department of Animal Sciences, Auburn University, Auburn, AL 36849 (United States)]. E-mail: muntirb@auburn.edu; Krupa, S.V. [Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108 (United States)

    2007-09-15

    Alfalfa (Medicago sativa) nutritive quality response to ambient ozone (O{sub 3}), sulfur dioxide (SO{sub 2}) and oxides of nitrogen (NO {sub x} ) were assessed at three locations in west-central Alberta, Canada (1998-2002). Yield data were segregated into high and low relative to overall median yield. Ozone concentrations (hourly median and 95th-percentile) and precipitation (P) contributed 69 and 29%, respectively, to the variability in crude protein (CP) concentration in low-yielding alfalfa, whereas mean temperature (T) and relative humidity (RH) collectively influenced 98% of the variation in CP in high-yielding alfalfa. Three-fourths of the accounted variation in relative feed value (RFV) of low-yielding alfalfa was attributable to P, T and RH, whereas median and 95th-percentile hourly O{sub 3} concentrations and SO{sub 2} and NO {sub x} exposure integrals contributed 25%. In contrast, air quality, (mainly O{sub 3}) influenced 86% of the accounted variation in RFV of high-yielding alfalfa, and T and P collectively contributed 14%. - Exposure to ambient concentrations of phytotoxic air pollutants affected nutritive quality of alfalfa for ruminant livestock in a yield-dependent manner.

  4. Preliminary Study on Effect of Herbicides on Alfalfa Yield and Weed Community Characteristics in Yellow River Delta, China

    Directory of Open Access Journals (Sweden)

    WANG Guo-liang

    2016-05-01

    Full Text Available Alfalfa(Medicago sativa is one of important legume forages worldwide. However, weed is the main factor limiting alfalfa production. Biomass quality and yield and stability of dry matter production during cultivation are directly associated with the interference of weeds which compete with alfalfa for water, light and nutrients. The use of herbicides is a good alternative for weed control. In order to control weed in alfalfa field with suitable herbicide in Yellow River delta, the effect of four herbicides(imazethapyr, quizalofop-p-ethyl, haloxyfop-r-methyl and oxyfluorfen with different concentration on afalfal yield and weed community characteristics were studied. The results showed that both imazethapyr and haloxyfop-r-methyl treatments could increase alfalfa yield, and the best herbicide application concentration was imazethapyr with 2 000 mL·hm-2 and haloxyfop-r-methyl with 700 mL·hm-2, but oxyfluorfen treatment would limit alfalfa growth significantly. Weed species numbers in the treatments of imazethapyr, quizalofop-p-ethyl and oxyfluorfen decreased significantly. Digitariasanguinalis, Portulacaoleracea and Echinochloacrusgalli were more difficult to control from specie important value in all treatments. Species diversity index decreased with higher herbicide concentration in all treatments. From this study, herbicide imazethapyr with 2 000 mL·hm-2 application concentration was the best weed control method, and the second one was haloxyfop-r-methyl with 700 mL·hm-2 application concentration.

  5. In vitro and in vivo antioxidant activity of alfalfa (Medicago sativa L.) on carbon tetrachloride intoxicated rats.

    Science.gov (United States)

    Al-Dosari, Mohammed S

    2012-01-01

    The present study was conducted to determine whether lyophilized aqueous extract of alfalfa, or Medicago sativa L. could exert antioxidant activity against carbon tetrachloride-induced oxidative stress and liver injury in rats. The hepatoprotective activity of alfalfa extract was determined by assessing the levels of serum transaminases, ALP, bilirubin and lipid profile. Further, the effect of the test substance on malondialdehyde (MDA), an end product of lipid peroxidation; antioxidant liver enzyme non-protein sulfhydryl (NP-SH); and total protein (TP) were also studied. Serum transaminase, ALP, bilirubin level, lipid profile and liver MDA were significantly elevated and the antioxidant status in liver NP-SH and TP contents were declined in animals treated with CCl (4) alone. Pretreatment with alfalfa and silymarin for three weeks prior to the administration of CCl (4) significantly prevented the increase in the serum levels of hepatic marker, LDL, VLDL levels enzymes and reduced oxidative stress indicated by elevated NP-SH and TP concentration. The histopathological examination of the livers also showed that the alfalfa extract reduced the incidence of liver lesions induced by CCl (4). The in vitro antioxidant assessment of alfalfa extract on DPPH and carotene-linoleic assays demonstrated a moderate antioxidant potential. Results suggest that the alfalfa extract possesses hepatoprotective and antioxidative stress properties possibly through its antioxidant phytochemical constituents and substantiates its use in various liver disorders as a hepatoprotector.

  6. Paenibacillus medicaginis sp. nov. a chitinolytic endophyte isolated from the root nodule of alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Lai, Wei-An; Hameed, Asif; Lin, Shih-Yao; Hung, Mei-Hua; Hsu, Yi-Han; Liu, You-Cheng; Shahina, Mariyan; Shen, Fo-Ting; Young, Chiu-Chung

    2015-08-04

    A Gram-stain-variable, short-rod-shaped, endospore-forming, strictly aerobic, non-motile, chitinolytic and endophytic bacterium, designated strain CC-Alfalfa-19T, exhibiting unusual bipolar appendages was isolated from the root nodule of alfalfa (Medicago sativa L.) in Taiwan and subjected to a polyphasic taxonomy. Based on 16S rRNA gene sequence analysis, strain CC-Alfalfa-19T was found to be most closely related to Paenibacillus puldeungensis CAU 9324T (95.2 %), whereas other Paenibacillus species shared ≤95.0 % sequence similarity. The phylogenetic analysis revealed a distinct phyletic lineage established by strain CC-Alfalfa-19T with respect to other Paenibacillus species. Fatty acids comprised predominantly anteiso-C15:0, C16:0, anteiso-C17:0 and iso-C16. Menaquinone 7 (MK-7) was identified as the sole respiratory quinone and the genomic DNA G+C content was 42.7 mol%. Polar lipids included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, an unidentified glycolipid and an unidentified lipid. The diagnostic diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid. Based on the polyphasic taxonomic evidences that were in line with the genus Paenibacillus and additional distinguishing characteristics, strain CC-Alfalfa-19T is considered to represent a novel species, for which the name Paenibacillus medicaginis sp. nov. (type strain CC-Alfalfa-19T = BCRC 80441T = JCM 18446T) is proposed.

  7. The Determinants of Plant Variety Protection Applications in China

    Institute of Scientific and Technical Information of China (English)

    Hu Ruifa; Huang Jie; Carl Prày; Huang Jikun

    2007-01-01

    China passed its Plant Variety Protection Act (PVPA) in 1997 which allowed us to do some initial testing of this proposition. We used a unique dataset on the applications of the new Plant Variety Protection (PVP), morphological characteristics, and institutional sources of all the important varieties of rice in three provinces of China to estimate the determinants of plant variety protection applications in China. The evidence suggests that both the government and private research programs are responding to economic and policy incentives and investing in plant variety protection as economists would expect profit maximizing firms to invest. The discussion of the evolution of the seed industry suggested that the combination of the new seed law in 2000 and the new plant variety protection regulation had changed the structure of the seed industry and provided an important incentive to invest in PVPs both by public research institutes and commercial firms. Finally, there is some preliminary evidence that private firms have smaller incentives to develop new varieties than to purchase the new varieties.

  8. ‘七月鲜’苹果不同着色度果实主要组成成分及其变化%Composition and changes of fruit main components during fruit developments of apple variety ‘ Qiyuexian'

    Institute of Scientific and Technical Information of China (English)

    宣景宏; 王冬梅; 刘志; 伊凯; 吕德国

    2012-01-01

    [Objective] The study is to determine the appropriate harvest time for ‘Qiyuexian'. [Method] Fruits with different shade of skin colour were used to investigate the dynamic changes in the fruit composition during its development. [Result]The results showed that there was not obvious difference in average single fruit weight and fruit shape index among green-ripe, commercial-ripe and full ripe fruit, but the soluble solids, total sugar, tannin, and anthocyanin contents were significantly higher while the fruit firmness and titratable acid content decreased significantly as the fruit was getting ripe. Fruit respiratory rate at the green ripe stage and full ripe stage was low, and reached the highest at the commercial ripe stage. There were 53 kinds of aroma components detected, mainly esters, alcohols, aldehydes, ketones, terpenes, and benzene, and the characteristic aroma components were butanoic acid ethyl ester, butanoic acid-2- methyl-ethyl ester, hexanoic acid ethyl ester, propanoic acid ethyl ester, ethyl alcohol, styrene, 3-methyl- 4-oxo-Pentanoic acid, toluene, o-xylene and ethylbenzene. [Conclusion]‘Qiyuexian' is a ester aroma variety and the optimum harvest time for it is at commercial-riDe state.%【目的】为了确定‘七月鲜’苹果的最佳采收时期,【方法】以该品种不同着色度果实为试材,通过室内测定研究了果实主要组成成分在果实发育过程的变化动态。【结果】结果表明,绿熟、商熟、完熟3种成熟度果实的平均单果质量、果形指数差异不明显,随果实成熟,可溶性固形物含量、总糖含量、单宁含量、果皮花青苷含量显著升高.果肉硬度、可滴定酸含量显著下降;果实呼吸速率在绿熟期和完熟期较低,商熟期达到最高。主要香气成分为酯类、醇类、醛类、酮类、萜烯类和苯类;特征香气成分为丁酸乙酯、2一甲基丁酸乙酯、已酸乙酯、丙酸乙酯、乙醇、苯乙烯、3

  9. Co-downregulation of the hydroxycinnamoyl-CoA:shikimate hydroxycinnamoyl transferase and coumarate 3-hydroxylase significantly increases cellulose content in transgenic alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Tong, Zongyong; Li, Heng; Zhang, Rongxue; Ma, Lei; Dong, Jiangli; Wang, Tao

    2015-10-01

    Lignin is a component of the cell wall that is essential for growth, development, structure and pathogen resistance in plants, but high lignin is an obstacle to the conversion of cellulose to ethanol for biofuel. Genetically modifying lignin and cellulose contents can be a good approach to overcoming that obstacle. Alfalfa (Medicago sativa L.) is rich in lignocellulose biomass and used as a model plant for the genetic modification of lignin in this study. Two key enzymes in the lignin biosynthesis pathway-hydroxycinnamoyl -CoA:shikimate hydroxycinnamoyl transferase (HCT) and coumarate 3-hydroxylase (C3H)-were co-downregulated. Compared to wild-type plants, the lignin content in the modified strain was reduced by 38%, cellulose was increased by 86.1%, enzyme saccharification efficiency was increased by 10.9%, and cell wall digestibility was increased by 13.0%. The modified alfalfa exhibited a dwarf phenotype, but normal above ground biomass. This approach provides a new strategy for reducing lignin and increasing cellulose contents and creates a new genetically modified crop with enhanced value for biofuel.

  10. Molecular cloning, characterization, and expression of an alfalfa (Medicago sativa L.) heme oxygenase-1 gene, MsHO1, which is pro-oxidants-regulated.

    Science.gov (United States)

    Fu, Guang-Qing; Xu, Sheng; Xie, Yan-Jie; Han, Bin; Nie, Li; Shen, Wen-Biao; Wang, Ren

    2011-07-01

    It has been documented that plant heme oxygenase-1 (HO-1; EC 1.14.99.3) is both development- and stress-regulated, thus it plays a vital role in light signalling and stress responses. In this study, an alfalfa (Medica sativa L.) HO-1 gene MsHO1 was isolated and sequenced. It contains four exons and three introns within genomic DNA sequence and encodes a polypeptide with 283 amino acids. MsHO1 had a conserved HO signature sequence and showed high similarity to other HOs in plants, especially HO-1 isoform. The MsHO1:GFP fusion protein was localized in the chloroplast. Further biochemical activity analysis of mature MsHO1, which was expressed in Escherichia coli, showed that the Vmax was 48.78 nmol biliverdin-IXα (BV) h⁻¹ nmol⁻¹ protein with an apparent Km value for hemin of 2.33 μM, and the optimum Tm and pH were 37 °C and 7.2, respectively. Results of semi-quantitative RT-PCR and western blot showed that the expressions of MsHO1 were higher in alfalfa stems and leaves than those in germinating seeds and roots. Importantly, MsHO1 gene expression and protein level were induced significantly by some pro-oxidant compounds, including hemin and nitric oxide (NO) donor sodium nitroprusside (SNP). In conclusion, MsHO1 may play an important role in oxidative responses.

  11. Resistance and susceptibility of alfalfa (Medicago sativa L.) cultivars to the aphid Therioaphis maculata (Homoptera:Aphididae): insect biology and cultivar evaluation

    Institute of Scientific and Technical Information of China (English)

    ALEXANDRE DE ALMEIDA E SILVA; ELENICE MOURO VARANDA; JOS(E) RICARDO BAROSELA

    2006-01-01

    Biology of the aphid Therioaphis maculata was studied on alfalfa (Medicago sativa L.), including four resistant (Mesa-Sirsa, CUF101, Baker and Lahontan) and two susceptible (ARC and Caliverde) alfalfa cultivars, and one of the most cropped Brazilian cultivars, Crioula. Under controlled conditions, antibiosis (i.e., reduced longevity, fecundity and increased mortality of the aphid) was observed mainly on the resistant alfalfa cultivars,except on Lahontan. Crioula seemed to be tolerant to aphids. Present data support geographic limitation usage of cultivars, and we suggest Baker and Mesa-Sirsa as sources of antibiosis,and provide biological information of a tropical T. maculata biotype on alfalfa.

  12. Affine Patches on Positroid Varieties and Affine Pipe Dreams (Thesis)

    CERN Document Server

    Snider, Michelle

    2010-01-01

    The objects of interest in this thesis are positroid varieties in the Grassmannian, which are indexed by juggling patterns. In particular, we study affine patches on these positroid varieties. Our main result corresponds these affine patches to Kazhdan-Lusztig varieties in the affine Grassmannian. We develop a new term order and study how these spaces are related to subword complexes and Stanley-Reisner ideals. We define an extension of pipe dreams to the affine case and conclude by showing how our affine pipe dreams are generalizations of Cauchon and Le diagrams.

  13. Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers

    Directory of Open Access Journals (Sweden)

    Ahsyee Salem R.

    2013-01-01

    Full Text Available Alfalfa (Medicago sativa L. is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population to 37 (Gabsia population. The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356. The dendrogram based on Ward’s minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.

  14. Best Phd thesis Prize: Statistical analysis of ALFALFA galaxies: insights in galaxy

    Science.gov (United States)

    Papastergis, E.

    2013-09-01

    We use the rich dataset of local universe galaxies detected by the ALFALFA 21cm survey to study the statistical properties of gas-bearing galaxies. In particular, we measure the number density of galaxies as a function of their baryonic mass ("baryonic mass function") and rotational velocity ("velocity width function"), and we characterize their clustering properties ("two-point correlation function"). These statistical distributions are determined by both the properties of dark matter on small scales, as well as by the complex baryonic processes through which galaxies form over cosmic time. We interpret the ALFALFA measurements with the aid of publicly available cosmological N-body simulations and we present some key results related to galaxy formation and small-scale cosmology.

  15. ALFALFA HI Content and Star Formation in Virgo Cluster Early-Type Dwarfs

    CERN Document Server

    Koopmann, R A; Haynes, M P; Brosch, N

    2009-01-01

    The ALFALFA (Arecibo Legacy Fast ALFA) blind survey is providing a census of HI in galaxies of all types in a range of environments. Here we report on ALFALFA results for Virgo Cluster early-type dwarfs between declinations of 4 and 16 degrees. Less than 2% of the Virgo early-type dwarf population is detected, compared to 70-80% of the Im/BCD dwarf population. Most of the dwarfs detected in HI show evidence for ongoing or recent star formation. Early-type galaxies with HI tend to be located in the outer regions of the cluster and to be brighter. Early-type dwarfs with HI may be undergoing morphological transition due to cluster environmental effects.

  16. On special subvarieties of Kuga varieties II

    CERN Document Server

    Chen, K

    2011-01-01

    In this paper we prove the equidistribution of $\\Cbf$-special subvarieties in certain Kuga varieties, which implies a special case of the general Andr\\'e-Oort conjecture formulated for mixed Shimura varieties proposed by R.Pink. The main idea is to reduce the equidistribution to a theorem of Szpiro-Ullmo-Zhang on small points of abelian varieties and a theorem on the equiditribution of $C$-special subvarieties of Kuga varieties of rigid type treated by the author in a previous paper.

  17. Identification and Selection for Salt Tolerance in Alfalfa (Medicago sativa L. Ecotypes via Physiological Traits

    Directory of Open Access Journals (Sweden)

    Hassan MONIRIFAR

    2009-12-01

    Full Text Available Salt stress is a serious environmental problem throughout the world which may be partially relieved by breeding cultivars that can tolerate salt stress. Plant breeding may provide a relatively cost effective short-term solution to the salinity problem by producing cultivars able to remain productive at low to moderate levels of salinity. Five alfalfa cultivars, �Seyah-Roud�, �Ahar-Hourand�, �Oskou�, �Malekan� and �Sefida-Khan� were assessed for salt tolerance at mature plant stage. A greenhouse screening system was used to evaluate individual alfalfa plants grown in perlit medium, and irrigated with water containing different amounts of NaCl. Three salt levels were achieved by adding 0, 100 and 200 mM NaCl to Hoagland nutrient solution, respectively. Forage yield, sodium and potassium contents and K/Na ratio was determined. Also, leaf samples were analyzed for proline and chlorophyll contents. The ecotypes Seyha-Roud and �Sefida-Khan� had comparatively less sodium contents than �Oskou�, �Ahar-Hourand� and �Malekan� ecotypes, also potassium content increased under saline condition. Forage yield of different alfalfa ecotypes was significantly influenced by the salinity. The ecotypes �Malekan�, Ahar- Hourand and �Oskou� were successful in maintaining forage yield under salinity stress. Sodium contents increased due to salinity in all alfalfa ecotypes however ecotypes �Ahar-Hourand� and �Malekan� maintained the highest leaf Na concentration. They showed higher content of K than other ecotypes but had lower K/Na ratio. It was concluded that, two ecotypes �Malekan� and �Ahar-Hourand� were better.

  18. The Arecibo Pisces-Perseus Survey: An Undergraduate ALFALFA Team Project

    Science.gov (United States)

    O'Donoghue, Aileen A.; Koopmann, Rebecca A.; Haynes, Martha P.; Jones, Michael; Craig, David; Hallenbeck, Gregory L.; Rosenberg, Jessica L.; Venkatesan, Aparna; Undergraduate ALFALFA Team

    2016-01-01

    The Milky Way's position in an outer filament of Lanieakea affords us a striking view of the Pisces-Perseus Supercluster (PPS) arcing roughly from 22h to 4h and 0° to +50° concentrated between cz = 4,000 km/s and cz = 8,000 km/s as a "wall" parallel to the plane of the sky. It is bounded by voids both between Laniakea and PPS and beyond PPS. Within this box, the 70% ALFALFA survey has detected 4,800 galaxies within cz = 8,000 km/s. Of these, 80% have masses greater than 108 M⊙. At the distance of the PPS, galaxies with MHI ≤ 108 M⊙ are below the ALFALFA detection limit. Thus to further explore this rich diversity of galaxy environments and the adjoining voids, the Undergraduate ALFALFA Team is in the process of using the L-band Wide receiver at Arecibo Observatory for the Arecibo Pisces-Perseus Supercluster Survey (APPSS). We will observe galaxies with 108 M⊙ ≤ MHI ≤ 109 M⊙ chosen from the SDSS DR12 and GALEX catalogs. We are limiting our observations to the PPS ridge in 21h 30m to 3h 15m and 23° to 35°. Since this region lacks SDSS spectroscopy, targets have been selected using photometric criteria derived from SDSS and GALEX observations for galaxies detected by ALFALFA. The results of these observations will allow us to constrain the HI mass function along the PPS ridge. Application of the Tully-Fisher relation will allow a robust measure of the infall velocities of galaxies into the filament. This work has been supported by NSF grant AST-1211005.

  19. Requirement of succinate dehydrogenase activity for symbiotic bacteroid differentiation of Rhizobium meliloti in alfalfa nodules.

    OpenAIRE

    Gardiol, A E; Truchet, G L; Dazzo, F. B.

    1987-01-01

    Transmission electron microscopy was used to study the cellular morphologies of a wild-type Rhizobium meliloti strain (L5-30), a nitrogen fixation-ineffective (Fix-) succinate dehydrogenase mutant (Sdh-) strain, and a Fix+ Sdh+ revertant strain within alfalfa nodules and after free-living growth in a minimal medium containing 27 mM mannitol plus 20 mM succinate. The results showed a requirement of succinate dehydrogenase activity for symbiotic differentiation and maintenance of R. meliloti ba...

  20. Changes in fatty acid composition of sulfolipid and phospholipids during maturation of alfalfa.

    Science.gov (United States)

    Klopfenstein, W E; Shigley, J W

    1967-07-01

    Lipids were extracted from alfalfa samples collected at intervals over the growing season and were fractionated to yield pure sulfolipid. In the sulfolipid and in a phospholipid fraction the major fatty acids were palmitic, linolenic, and linoleic, of which the palmitic acid increased in proportion during the season while the proportion of linolenic acid dropped. The sulfolipid contained more linolenic acid and less palmitic and linoleic acids than the phospholipids, and had a greater rate of change of fatty acid composition.

  1. Expression of a fungal ferulic acid esterase in alfalfa modifies cell wall digestibility

    Science.gov (United States)

    2014-01-01

    Background Alfalfa (Medicago sativa) is an important forage crop in North America owing to its high biomass production, perennial nature and ability to fix nitrogen. Feruloyl esterase (EC 3.1.1.73) hydrolyzes ester linkages in plant cell walls and has the potential to further improve alfalfa as biomass for biofuel production. Results In this study, faeB [GenBank:AJ309807] was synthesized at GenScript and sub-cloned into a novel pEACH vector containing different signaling peptides to target type B ferulic acid esterase (FAEB) proteins to the apoplast, chloroplast, endoplasmic reticulum and vacuole. Four constructs harboring faeB were transiently expressed in Nicotiana leaves, with FAEB accumulating at high levels in all target sites, except chloroplast. Stable transformed lines of alfalfa were subsequently obtained using Agrobacterium tumefaciens (LBA4404). Out of 136 transgenic plants regenerated, 18 independent lines exhibited FAEB activity. Subsequent in vitro digestibility and Fourier transformed infrared spectroscopy (FTIR) analysis of FAEB-expressing lines showed that they possessed modified cell wall morphology and composition with a reduction in ester linkages and elevated lignin content. Consequently, they were more recalcitrant to digestion by mixed ruminal microorganisms. Interestingly, delignification by alkaline peroxide treatment followed by exposure to a commercial cellulase mixture resulted in higher glucose release from transgenic lines as compared to the control line. Conclusion Modifying cell wall crosslinking has the potential to lower recalcitrance of holocellulose, but also exhibited unintended consequences on alfalfa cell wall digestibility due to elevated lignin content. The combination of efficient delignification treatment (alkaline peroxide) and transgenic esterase activity complement each other towards efficient and effective digestion of transgenic lines. PMID:24650274

  2. Genome-wide SNP discovery in tetraploid alfalfa using 454 sequencing and high resolution melting analysis

    Directory of Open Access Journals (Sweden)

    Zhao Patrick X

    2011-07-01

    Full Text Available Abstract Background Single nucleotide polymorphisms (SNPs are the most common type of sequence variation among plants and are often functionally important. We describe the use of 454 technology and high resolution melting analysis (HRM for high throughput SNP discovery in tetraploid alfalfa (Medicago sativa L., a species with high economic value but limited genomic resources. Results The alfalfa genotypes selected from M. sativa subsp. sativa var. 'Chilean' and M. sativa subsp. falcata var. 'Wisfal', which differ in water stress sensitivity, were used to prepare cDNA from tissue of clonally-propagated plants grown under either well-watered or water-stressed conditions, and then pooled for 454 sequencing. Based on 125.2 Mb of raw sequence, a total of 54,216 unique sequences were obtained including 24,144 tentative consensus (TCs sequences and 30,072 singletons, ranging from 100 bp to 6,662 bp in length, with an average length of 541 bp. We identified 40,661 candidate SNPs distributed throughout the genome. A sample of candidate SNPs were evaluated and validated using high resolution melting (HRM analysis. A total of 3,491 TCs harboring 20,270 candidate SNPs were located on the M. truncatula (MT 3.5.1 chromosomes. Gene Ontology assignments indicate that sequences obtained cover a broad range of GO categories. Conclusions We describe an efficient method to identify thousands of SNPs distributed throughout the alfalfa genome covering a broad range of GO categories. Validated SNPs represent valuable molecular marker resources that can be used to enhance marker density in linkage maps, identify potential factors involved in heterosis and genetic variation, and as tools for association mapping and genomic selection in alfalfa.

  3. Concerted changes in N and C primary metabolism in alfalfa (Medicago sativa) under water restriction

    OpenAIRE

    Aranjuelo, Iker; Tcherkez, Guillaume,; Molero, Gemma; Gilard, Françoise; AVICE, JEAN-CHRISTOPHE; Nogués, Salvador

    2013-01-01

    Although the mechanisms of nodule N2 fixation in legumes are now well documented, some uncertainty remains on the metabolic consequences of water deficit. In most cases, little consideration is given to other organs and, therefore, the coordinated changes in metabolism in leaves, roots, and nodules are not well known. Here, the effect of water restriction on exclusively N2-fixing alfalfa (Medicago sativa L.) plants was investigated, and proteomic, metabolomic, and physiological analyses were ...

  4. Effect of feeding selenium-fertilized alfalfa hay on performance of weaned beef calves.

    Directory of Open Access Journals (Sweden)

    Jean A Hall

    Full Text Available Selenium (Se is an essential micronutrient in cattle, and Se-deficiency can affect morbidity and mortality. Calves may have greater Se requirements during periods of stress, such as during the transitional period between weaning and movement to a feedlot. Previously, we showed that feeding Se-fertilized forage increases whole-blood (WB Se concentrations in mature beef cows. Our current objective was to test whether feeding Se-fertilized forage increases WB-Se concentrations and performance in weaned beef calves. Recently weaned beef calves (n = 60 were blocked by body weight, randomly assigned to 4 groups, and fed an alfalfa hay based diet for 7 wk, which was harvested from fields fertilized with sodium-selenate at a rate of 0, 22.5, 45.0, or 89.9 g Se/ha. Blood samples were collected weekly and analyzed for WB-Se concentrations. Body weight and health status of calves were monitored during the 7-wk feeding trial. Increasing application rates of Se fertilizer resulted in increased alfalfa hay Se content for that cutting of alfalfa (0.07, 0.95, 1.55, 3.26 mg Se/kg dry matter for Se application rates of 0, 22.5, 45.0, or 89.9 g Se/ha, respectively. Feeding Se-fertilized alfalfa hay during the 7-wk preconditioning period increased WB-Se concentrations (P Linear<0.001 and body weights (P Linear = 0.002 depending upon the Se-application rate. Based upon our results we suggest that soil-Se fertilization is a potential management tool to improve Se-status and performance in weaned calves in areas with low soil-Se concentrations.

  5. RB975242 and RB975201 - Late maturation sugarcane varieties

    Directory of Open Access Journals (Sweden)

    Monalisa Sampaio Carneiro

    2016-11-01

    Full Text Available The sugarcane varieties RB975201 and RB975242 were developed and released for harvest at the end of the season (late maturation in the CentralSouth region of Brazil. In specific environments, these varieties were compared with commercial standards in sugar yield per area. They are resistant to major sugarcane diseases and present the Bru1 gene of resistance to brown rust.

  6. Characterization of Feeding Injuries Caused by Ceresa nigripectus Remes Lenicov (Hemiptera: Membracidae) on Alfalfa Stems.

    Science.gov (United States)

    Grosso, T P; Mercado, M I; Ponessa, G I; Conci, L R; Virla, E G

    2016-04-01

    Piercing-sucking insects cause mechanical and physiological injury to plants. Ceresa nigripectus Remes Lenicov is a pest of alfalfa in subtropical regions of South America and a carrier of the ArAWB phytoplasma. The aim of this study was to determine the feeding habits of this treehopper and to describe the effects of the feeding injuries on stem vascular tissues in alfalfa. Adults and nymphs of C. nigripectus inserted their stylets repeatedly girdling the stem. One week after feeding, alfalfa stems exhibited numerous feeding canals with salivary deposits, most of which reached the phloem. Two weeks after feeding, cortex and phloem cells next to the salivary sheath collapsed, mature tracheal elements became sparse and appeared with an increased cross-section area, and phenolic compounds increased in cells and cell walls compared to undamaged plants. Three weeks after feeding, an annular callus, formed by abnormal cell division and hypertrophy of preexisting cortex and vascular cambium cells, appeared immediately above the stem girdle. Parenchyma cells from the outer layers of the callus differentiated to form secondary anomalous amphicribal bundles in the wound. The aerial parts above the stem girdle eventually withered and died.

  7. Application limestone forms and doses for alfalfa in no-tillage system

    Directory of Open Access Journals (Sweden)

    Letícia Cristina Bertusso Toffolli

    Full Text Available Alfalfa (Medicago sativa L. requires good soil fertility. Brazil is characterized by acidic soils which reduce the potential of the crop. Generally, liming is incorporated into the soil, but in tillage systems it is inadvisable. This study aimed to evaluate the effects of the lime application method and dose on pH, Al+3, V % and Ca+Mg in the soil and on dry matter yield of alfalfa cultivated under a consolidated no-tillage system. The experiment was conducted at the Experimental Station of Paraná Agronomic Institute, located in Pato Branco city, in Paraná state. The plots consisted of the types of lime application (plowing+harrowing, subsoil and surface, the sub-plots was the lime dose (0, 2, 4, 6 and 8 Mg ha-1 and the sub-sub-plots were the sampled soil depth (0-5; 5-10; 10-20 and 20-30 cm. The results show the application of lime, even superficially, caused increases in pH, concentration of Ca and Mg and base saturation of the soil, while also reducing the concentration of Al, especially in the surface layers of the soil. The practice of plowing and harrowing or of subsoiling, with the aim of lime incorporation in a consolidated no-tillage system is unnecessary. If it is required, the application of lime to the soil should be done superficially for alfalfa cultivated in this system.

  8. Enhanced salt tolerance of alfalfa (Medicago sativa) by rstB gene transformation.

    Science.gov (United States)

    Zhang, Wan-Jun; Wang, Tao

    2015-05-01

    Generating salt tolerance forage plant is essential for use of the land affected by high salinity. A salt tolerance gene rstB was used as a selectable marker gene in Agrobacterium-mediated transformation of tobacco under a selective regime of 170mM NaCl. The transgenic plants showed clear improvement in salt tolerance. To improve salt tolerance of alfalfa (Medicago sativa L.), rstB gene was introduced into alfalfa genome by Agrobacterium-mediated transformation. No abnormal phenotype was observed among the transgenic plants when compared with wild type (wt) plants. Significant enhancement of resistance to salt-shock treatment was noted on the rstB transgenic (T0) plants. Transgenic second-generation (T1) seeds showed improved germination rate and seedling growth under salt-stress condition. Hindered Na(+) accumulation, but enhanced Ca(2+) accumulation was observed on the rstB T1 plants when subjected to salt-stresses. Enhanced calcium accumulation in transgenic plants was also verified by cytohistochemical localization of calcium. Under salt-stress of 50mM NaCl, about 15% of the transgenic plants finished their life-cycle but the wt plants had no flower formation. The results demonstrated that the expression of rstB gene improved salt tolerance in transgenic alfalfa.

  9. Association of AFLP and SCAR markers with common leafspot resistance in autotetraploid alfalfa (Medicago sativa).

    Science.gov (United States)

    Wang, Y; Bi, B; Yuan, Q H; Li, X L; Gao, J M

    2012-03-14

    To identify amplified fragment length polymorphism (AFLP) markers associated with resistance or susceptibility of alfalfa to common leafspot (CLS) caused by the fungus Pseudopeziza medicaginis (Dermateaceae), bulked segregant analysis was conducted based on an F(1(M × M)) population of 93 plants and a BC(1)S population of 91 plants. Three AFLP markers, ACTCAA(R206), TAGCAC(R185), and GGACTA(S264), were found to be associated with CLS resistance or susceptibility. All three markers were found at significantly different frequencies (71.9, 80.3 and 91.8%) compared to resistant or susceptible plants in the original population. Subsequently, these three AFLP markers were converted into three SCAR markers, ACTCAA(R136), TAGCAC(R128) and GGACTA(S254), which are easier to employ in breeding programs. The three SCAR markers were used in a randomly selected population with 50% resistance; the probability of finding one resistant plant was increased to 67.3, 66.7 and 90.0% with markers ACTCAA(R136), TAGCAC(R128) and GGACTA(S254), independently. If two of the SCAR markers were used simultaneously, the probability would be higher than 89%. The three SCAR markers identified in this study would be applicable for selection for CLS resistance in alfalfa breeding programs. Moreover, the genetic analysis indicated that CLS resistance in alfalfa is conferred by a single dominant gene.

  10. Isolation, chemical characterization and biological activity of alfalfa (Medicago media Pers. root saponins

    Directory of Open Access Journals (Sweden)

    Wiesław Oleszek

    2014-01-01

    Full Text Available Saponins from alfalfa (Medicago media Pers. roots were isolated and their acid hydrolysis revealed several aglycones that were obtained in crystalline form and characterized. Medicagenic acid, hederagenin and soyasapogenols A. B, C. D, E and F were found. Crude saponins were separated into cholesterol-precipitable and nonprecipitable fractions. The precipitable fraction consisted of medicagenic acid glycosides: glucose, arabinose, xylose and rhamnose were found as their sugar chain components. The nonprecipitable fraction was a mixture of hederagenine and soyasapogenol glycosides, and glucose arthinose. xylose. galactose and glucuronic acid were found in the sugar component,. 7 he medicagenic acid glycosides made up 6% of root dry matter and showed high biological activity. They cuased red blood cells lysis (haemolytic index 3000, completely inhibited Trichoderma viride growth at the concentration of 2.5 Mg. 100 cm-3 of growth medium and retarded wheat seedling growth at concentrations as low as 100 ppm. The cholesterol-nonprecipitable fraction caused no blood cell lysis and fungus growth inhibition, although it did inhibit seedling growth, but to a much lesser extent than medicagenic acrd glycosides. The detrimental effect of alfalfa root saponins on winter wheat crop after alfalfa is discussed.

  11. [Composition diversity of lactic acid bacteria (LAB) community Al2 used for alfalfa silage].

    Science.gov (United States)

    Wang, Xiao-Fen; Gao, Li-Juan; Yang, Hong-Yan; Wang, Wei-Dong; Cui, Zong-Jun

    2006-10-01

    Alfalfa is the most important forage grass that is difficult to ensile for good quality. Using silage inoculants are the important way for preservation of alfalfa silage. Through continuous restricted subcultivation, a lactic acid bacteria (LAB) community Al2 was selected from well-fermented alfalfa silage. Plate isolation and Denaturing Gradient Gel Electrophoresis (DGGE), construction of 16S rDNA clone library were used to identify the composition diversity of Al2 community, with 7 strains detected, and they were all belonged to Lactobacillus. The composition ratios of the 7 strains were 55.21%, 19.79%, 14.58%, 3.13%, 3.13%, 3.13%, 1.03% according to 16S rDNA clone library. Al2-1i, Al2-2i, Al2-3i, corresponding to L. plantarum (99.9%), L. kimchii (99.4%), L. farciminis (100%) were detected by plate isolation. Among 3 isolates, Al2-1i had the highest ability of dropping pH and producing lactic acid, and the amount of lactic acid was reach to 18g/L at 24h cultivated in MRS media. The ability of dropping pH and producing lactic acid of Al2-3i was the lowest. From DGGE profiles, the dominant strains in Al2 community were L. plantarum and L. kimchii. L. plantarum was detected during the whole process, and L. kimchii was detected in the later phase.

  12. Inactivation of Human Norovirus and Its Surrogates on Alfalfa Seeds by Aqueous Ozone.

    Science.gov (United States)

    Wang, Qing; Markland, Sarah; Kniel, Kalmia E

    2015-08-01

    Alfalfa sprouts have been associated with numerous foodborne outbreaks. Previous studies investigated the effectiveness of aqueous ozone on bacterially contaminated seeds, yet little is known about the response of human norovirus (huNoV). This study assessed aqueous ozone for the disinfection of alfalfa seeds contaminated with huNoV and its surrogates. The inactivation of viruses without a food matrix was also investigated. Alfalfa seeds were inoculated with huNoV genogroup II, Tulane virus (TV), and murine norovirus (MNV); viruses alone or inoculated on seeds were treated in deionized water containing 6.25 ppm of aqueous ozone with agitation at 22°C for 0.5, 1, 5, 15, or 30 min. The data showed that aqueous ozone resulted in reductions of MNV and TV infectivity from 1.66 ± 1.11 to 5.60 ± 1.11 log PFU/g seeds; for all treatment times, significantly higher reductions were observed for MNV (P water was similar to that of huNoV, whereas MNV had significantly greater reductions in genomic copies (P ozone-treated viruses alone, with significantly higher levels of inactivation (P ozone indicates that ozone may be a plausible substitute for chlorine as an alternative treatment for seeds. The behavior of TV was similar to that of huNoV, which makes it a promising surrogate for these types of scenarios.

  13. Classification of rank 2 cluster varieties

    DEFF Research Database (Denmark)

    Mandel, Travis

    We classify rank 2 cluster varieties (those whose corresponding skew-form has rank 2) according to the deformation type of a generic fiber U of their X-spaces, as defined by Fock and Goncharov. Our approach is based on the work of Gross, Hacking, and Keel for cluster varieties and log Calabi...

  14. Innovativeness and Variety of Internet Shopping.

    Science.gov (United States)

    Blake, Brian F.; Neuendorf, Kimberly A.; Valdiserri, Colin M.

    2003-01-01

    This survey of 208 Internet users examined the factors underlying Internet usage and shopping. Data were gathered on Information shopping (IS) innovativeness, overall IS frequency, visit variety, purchase variety, network prevalence, education, age, gender, employment, extensiveness of Internet use, and non-shopping Internet applications. (MES)

  15. Skill Variety, Innovation and New Business Formation

    NARCIS (Netherlands)

    S.J.A. Hessels (Jolanda); U. Brixy (Udo); W.A. Naudé (Wim); T. Gries (Thomas)

    2014-01-01

    markdownabstract__Abstract__ We extend Lazear’s theory of skills variety and entrepreneurship in three directions. First, we provide a theoretical framework linking new business creation with an entrepreneur’s skill variety. Second, in this model we allow for both generalists and specialists to pos

  16. Universal Grammar and the Basic Variety.

    Science.gov (United States)

    Bierwisch, Manfred

    1997-01-01

    Discusses the feasibility of Basic Variety (BV) principles proposed by Klein and Perdue, arguing that some of them need clarification with learner varieties and that they are not part of Universal Grammar (UG) as they exclude phenomena (e.g., psych verbs) that cannot be excluded from the core of natural language. (15 references) (Author/CK)

  17. NEWEST PINK BERRY AROMATIC VINE GRAPE VARIETY – RADOST LEONIDOV

    Directory of Open Access Journals (Sweden)

    Zamanidi P. C.

    2014-12-01

    Full Text Available New wine grape flavored pink berry grape variety named “Radost Leonidov” breed at Athens Institute of Viticulture by P.Zamanidi, L.Troshin and P.Radchevskii in 2009 by crossing the new Moskhoragos Greek muscat variety with old European variety Traminer pink. According to morphological and biological characteristics, it can be assigned to Western European eco-geographical group of varieties. Strong growth of shoots (2,1-3,0 m. The extent of maturation of vines is very high (over the entire length, except for the top. Duration of production period from bud burst to harvest - 146-155 days. Productivity is high: 20-25 t / ha. The average weight of clusters is 260. Characterized by high winter hardiness, drought resistance and increased resistance to fungal diseases in comparison with the varieties of Vitis vinifera. Flower is hermaphrodite, fully developed stamens and gynoecium. Bunch is small, conical, sometimes winged, average density. Berry is small, spherical, pinkish in color, with a thick waxy coating. Peel of medium thickness, dense and durable. Pulp and juice with a strong varietal flavor, similar to the aroma of Traminer pink. Sugar content is very high: more than 24%. Cluster of the variety of Radost Leonidov at overripening is drying and stored on the bushes for a long time. The variety is intended for the production of dry white wines of excellent class, as well as highquality sparkling wines, dessert and sweet wines; suitable for the production of high-quality aromatic juices

  18. TABLE GRAPE VARIETIES CULTURE TO NRDIBH STEFANESTI, OVER TIME

    Directory of Open Access Journals (Sweden)

    Adriana Costescu

    2013-07-01

    Full Text Available At NRDIBH Stefanesti-Arges, in the past decades extensive work has been done to improve the grape-vine, which yielded some remarkable results. The first concerns the improvement of grape-vines to NRDIBH Stefanesti-Arges (then SCPVV Stefanesti were initiated by the breeder engineer Baditescu Margareta, improvement in the Laboratory in 1982, was its founder, who has dedicated so much to obtain new varieties by crossing sexual and clonal selection to. Improvement work was continued by researchers: Smaranda Julia, Smaranda George, Camelia Popa. After lengthy activity at SCDVV Stefanesti were obtained clones new to old varieties of wine, but also at the table. Thus, clonal selections have been approved for 13 red and white wines. For table varieties were approved Argessis and Golden Stefanesti varieties and clones Stefanesti, Muscat Adda 22 and Perlette 10. Varieties approved quality characteristics that allow for superior wines, varieties of wine for the table and the quality of the grapes is good looks for Romania to meet the demands of markets in developed countries. Spread production of new varieties of table grapes, can effectively contribute to the recovery in activity in wine country by increasing efficiency and profitability.

  19. Effect of supplemental tallow on performance of dairy cows fed diets with different corn silage:alfalfa silage ratios.

    Science.gov (United States)

    Onetti, S G; Shaver, R D; McGuire, M A; Palmquist, D L; Grummer, R R

    2002-03-01

    A study was conducted to investigate the response to supplemental tallow of lactating cows fed basal diets with different alfalfa silage:corn silage ratios. We postulated that supplemental tallow will have decreasing negative effects on rumen fermentation, dry matter intake (DMI), and milk fat percentage as the dietary ratio of alfalfa silage:corn silage is increased. Eighteen Holstein cows averaging 134 +/- 14 d in milk were used in a replicated 6 x 6 Latin square design with 21-d periods. Treatments were arranged as a 2 x 3 factorial with 0 or 2% tallow (DM basis) and three forage treatments: 1) 50% of diet DM as corn silage, 2) 37.5% corn silage and 12.5% alfalfa silage, and 3) 25% corn silage and 25% alfalfa silage. Cows were allowed ad libitum consumption of a total mixed ration. Diets were formulated to contain 18% crude protein and 32% neutral detergent fiber. No fat x forage treatment interactions were observed. Fat supplemented cows had lower DMI and produced more milk with less milk fat content relative to non-supplemented cows. Concentration of trans-octadecenoic acids was higher in milk fat of tallow-supplemented cows. Tallow supplementation had no effect on ruminal pH and acetate:propionate ratio, but tended to decrease total volatile fatty acid (VFA) concentration in the rumen. Increasing the proportion of alfalfa silage increased DMI, milk fat percentage, and milk fat yield regardless of the fat content of the diet. Total VFA concentration and acetate:propionate ratio in the rumen were increased in response to higher levels of alfalfa in the diets. These results suggest that replacing corn silage with alfalfa silage did not alleviate the negative response of dairy cows to tallow supplementation at 2% of diet DM.

  20. Combination treatments for killing Escherichia coli O157:H7 on alfalfa, radish, broccoli, and mung bean seeds.

    Science.gov (United States)

    Bari, M L; Nei, D; Enomoto, K; Todoriki, S; Kawamoto, S

    2009-03-01

    In this study, the effectiveness of prolonged dry-heat treatment (50 degrees C) alone or in combination with chemical treatments (1% oxalic acid, 0.03% phytic acid, 50% ethanol, electrolyzed acidic water, and electrolyzed alkaline water) in eliminating Escherichia coli O157:H7 on laboratory-inoculated alfalfa, radish, broccoli, and mung bean seeds was compared with that of dry-heat treatment in combination with irradiation treatment. Dry-heat treatment for 17 or 24 h alone could reduce E. coli O157:H7 numbers to below detectable levels in radish, broccoli, and alfalfa seeds, but was unable to reduce the pathogen numbers to below the detectable level in mung bean seeds. In addition, dry-heat treatment for 17 h plus sanitizer treatments were effective in greatly reducing pathogen populations on radish, broccoli, and alfalfa seeds, without compromising the quality of the sprouts, but these treatments did not eliminate the pathogen from radish and alfalfa seeds. Seventeen hours of dry heat followed by a 1.0-kGy dose of irradiation completely eliminated E. coli O157:H7 from radish and mung bean seeds, whereas only a minimum radiation dose of 0.25 kGy was required to completely eliminate the pathogen from broccoli and alfalfa seeds. Dry heat in combination with radiation doses of up to 1.0 kGy did not negatively impact the seed germination rate or length of alfalfa, broccoli, and radish seeds or the length of alfalfa, broccoli, and radish sprouts, but did decrease the length of mung bean sprouts.

  1. Addition of fructooligosaccharides and alfalfa hay to the diet of weaned piglets on the microbiota and the small intestine morphology

    Directory of Open Access Journals (Sweden)

    Fabio Enrique Lemos Budiño

    2013-01-01

    Full Text Available The goal of this work was to verify the effect of the fructooligossaccharide prebiotic (FOS addition in the diet containing varying levels of alfalfa hay, on the microbiology and intestinal morphology of weaned piglets. It was used 72 piglets, crossed, both sexes, with initial weight of 5.95 ± 0.73 Kg, with approximate age of 21 days. It was evaluated the treatment: T1 – Basal feed + 0% FOS; T2 – Basal feed + 0.3% FOS; T3 – Feed with 5% of alfalfa + 0% FOS; T4 – Feed with 5% of alfalfa + 0.3% FOS; T5 – Feed with 10% of alfalfa + 0% FOS; Feed with 10% of alfalfa + 0.3% FOS. In the end of the experimental period one piglet of each replication was slaughtered for the collection of sample aiming to analyze Salmonela sp. and coliformes and intestinal morphology (height of villus and depht of crypt. The analysis for coliformes didn’t show statistic difference among the levels of FOS and alfalfa hay. It wasn’t find Salmonella sp. colonies in the samples. No difference was observe in the crypt depht (P>0.05 among the treatments. The height of villus and the relation of height of villus per depht of crypt, the group without the addition of FOS didn’t show difference (P>0.05, however, the group supplemented with 0.3% of FOS showed a significant improvement (P<0.05 related to levels of 5% and 10% of alfalfa. There was no influence of treatment on the presence of Salmonella sp. and E. Coli. Regarding intestinal morphology data for villus height and villus height ratio/crypt depth showed the best results in the presence of FOS.

  2. NUTRITIONAL CHARACTERISTICS OF EMMER WHEAT VARIETIES

    Directory of Open Access Journals (Sweden)

    Magdaléna Lacko - Bartošová

    2015-02-01

    Full Text Available The objective of this study was to evaluate the nutritional compounds (fat, sugars, crude protein, soluble fiber, ash and starch of four emmer wheat varieties grown under the conditions of organic farming system. The experiment was established on Scientific Research base Dolná Malanta, near Nitra in Slovakia during 2010 – 2011 and 2011 – 2012 growing seasons. Nutritional parameters, except crude protein content, were not influenced by the variety and weather conditions. Agnone variety had the highest content of fat, crude protein and starch but the lowest content of soluble dietary fiber. The lowest values of fat, crude protein had Molise sel Colli variety; Farvento variety had the lowest sugars and starch content. Emmer wheat as ancient wheat has a unique composition in secondary components, such as starch, which may play a role as functional food ingredients.

  3. Comparison of gas analyzers for quantifying eddy covariance fluxes- results from an irrigated alfalfa field in Davis, CA

    Science.gov (United States)

    Chan, S.; Biraud, S.; Polonik, P.; Billesbach, D.; Hanson, C. V.; Bogoev, I.; Conrad, B.; Alstad, K. P.; Burba, G. G.; Li, J.

    2015-12-01

    The eddy covariance technique requires simultaneous, rapid measurements of wind components and scalars (e.g., water vapor, carbon dioxide) to calculate the vertical exchange due to turbulent processes. The technique has been used extensively as a non-intrusive means to quantify land-atmosphere exchanges of mass and energy. A variety of sensor technologies and gas sampling designs have been tried. Gas concentrations are commonly measured using infrared or laser absorption spectroscopy. Open-path sensors directly sample the ambient environment but suffer when the sample volume is obstructed (e.g., rain, dust). Closed-path sensors utilize pumps to draw air into the analyzer through inlet tubes which can attenuate the signal. Enclosed-path sensors are a newer, hybrid of the open- and closed-path designs where the sensor is mounted in the environment and the sample is drawn through a short inlet tube with short residence time. Five gas analyzers were evaluated as part of this experiment: open-path LI-COR 7500A, enclosed-path LI-COR 7200, closed-path Picarro G2311-f, open-path Campbell Scientific IRGASON, and enclosed-path Campbell Scientific EC155. We compared the relative performance of the gas analyzers over an irrigated alfalfa field in Davis, CA. The field was host to a range of ancillary measurements including below-ground sensors, and a weighing lysimeter. The crop was flood irrigated and harvested monthly. To compare sensors, we evaluated the half-hour mean and variance of gas concentrations (or mole densities). Power spectra for the gas analyzers and turbulent fluxes (from a common sonic anemometer) were also calculated and analyzed. Eddy covariance corrections will be discussed as they relate to sensor design (e.g., density corrections, signal attenuation).

  4. Model of high-productive varieties in forage pea

    Directory of Open Access Journals (Sweden)

    Valentin Kosev

    2015-06-01

    Full Text Available A linear equation of regression was used for establishment of the influence of quantitative characteristics on the grain productivity in forage pea and for development of a model for breeding work. The model for pea plant with high productivity was characterized by average height of 60–70 cm, 8–10 formed pods, 30–40 seeds per plant and 160–260 g in regard to 1000-seed weight. The obtained results showed that the greatest effect on grain productivity had the seed number per plant, first pod height and 1000-seed weight. Kristal variety had high ecological plasticity and could be considered as close to an ideal type, suitable for growing under wide range of environments. Pleven 4 and Rezonator were determined as high-productive varieties and with low stability, Kerpo and Pikardi - as low-productive but stable varieties. Druzba was identified as unstable and low-productive variety.

  5. Oxidative stability of chicken meat during storage influenced by the feeding of alfalfa meal

    Directory of Open Access Journals (Sweden)

    Jana Tkáčová

    2015-05-01

    Full Text Available The aim of our experiment was to determine the effect of the alfalfa meal component in feed mixtures of Ross broiler chickens on oxidative stability of meat. Proportion of alfalfa meal in feed mixtures was 4 and 6%. The results were compared to the control group without alfalfa meal in feed mixtures. At the end of the experiment (day 38, 6 pcs of broiler chickens from each group with an average live body weight over 1 800 g were randomly selected. The samples for chemical analysis consisted of identical proportion of breast and thigh muscle, and about 1 cm2 of skin with subcutaneous fat. Fat from the meat was obtained after the samples drying. A fat was determined by extraction by means of laboratory instrument Det N Gras Selecta P. The oxidative stability of meat on the basis of acid number of fat was determided by chemical analysis. Chicken meat was stored at -18 °C for 12 months and 18 months. The acid number of fat of stored meat for 12 months was 7.38 mg KOH per g in the control group, 7.42 mg KOH per g in the group with a proportion of 4% alfalfa meal, and 11.18 mg KOH per g in the group with proportion 6% alfalfa meal. An acid number of fat of stored meat for 18 months was 5.90 mg KOH per g in the control group, 4.65 mg KOH per g in the group with a proportion of 4% alfalfa meal, and 7.07 mg KOH per g in the group with a proportion of 6% alfalfa meal. Chicken meat is notably sensitive to lipid oxidation because of its high content of polyunsaturated fatty acids. Legislation in Title 5 of Part 3 of the Codex Alimentarius of the Slovak Republic and the Government Regulation No. 286/2003 Coll. in the Annex 4 in Part B provide the requirements for animal fats and meat products. Regulation of the European Parliament and Council (EC No. 853/2004 lays down specific hygiene rules for food of animal origin. In particular, determination of free fatty acids content of rendered animal fat (tallow, lard, other animal fat. Legislative regulation does

  6. Flower volatiles, crop varieties and bee responses.

    Directory of Open Access Journals (Sweden)

    Björn K Klatt

    Full Text Available Pollination contributes to an estimated one third of global food production, through both the improvement of the yield and the quality of crops. Volatile compounds emitted by crop flowers mediate plant-pollinator interactions, but differences between crop varieties are still little explored. We investigated whether the visitation of crop flowers is determined by variety-specific flower volatiles using strawberry varieties (Fragaria x ananassa Duchesne and how this affects the pollination services of the wild bee Osmia bicornis L. Flower volatile compounds of three strawberry varieties were measured via headspace collection. Gas chromatography showed that the three strawberry varieties produced the same volatile compounds but with quantitative differences of the total amount of volatiles and between distinct compounds. Electroantennographic recordings showed that inexperienced females of Osmia bicornis had higher antennal responses to all volatile compounds than to controls of air and paraffin oil, however responses differed between compounds. The variety Sonata was found to emit a total higher level of volatiles and also higher levels of most of the compounds that evoked antennal responses compared with the other varieties Honeoye and Darselect. Sonata also received more flower visits from Osmia bicornis females under field conditions, compared with Honeoye. Our results suggest that differences in the emission of flower volatile compounds among strawberry varieties mediate their attractiveness to females of Osmia bicornis. Since quality and quantity of marketable fruits depend on optimal pollination, a better understanding of the role of flower volatiles in crop production is required and should be considered more closely in crop-variety breeding.

  7. 五个紫花苜蓿品种的引种试验研究%Study on Introduction of Five Alfalfa Varieties

    Institute of Scientific and Technical Information of China (English)

    王瑞; 桂枝; 陈艳勇

    2005-01-01

    选用甘农3号(Medicago sativa.L.Cv.Gannong No.3)、新疆大叶(Medicago sativa L.Cv.Xinjiang Daye)、爱乐高(Medicago sativa.L.Cv.Arogn)、陇东(Medicago sativa L.Cv.Longdong)、中苜一号(Medicago sativa L.Cv.Zhongmu No.1)共5个紫花苜蓿品种进行了发芽率试验、鲜草产量调查及品质分析.结果表明,中苜一号苜蓿品种综合性状最佳,表现出明显的耐盐性,适合在天津中低盐碱地种植;新疆大叶品种产草量高,有一定的耐盐特性,在该区同样具有良好的适应性.

  8. Chemical and irradiation treatments for killing Escherichia coli O157:H7 on alfalfa, radish, and mung bean seeds.

    Science.gov (United States)

    Bari, M L; Nazuka, E; Sabina, Y; Todoriki, S; Isshiki, K

    2003-05-01

    In this study, the effectiveness of dry-heat treatment in combination with chemical treatments (electrolyzed oxidizing [EO] water, califresh-S, 200 ppm of active chlorinated water) with and without sonication in eliminating Escherichia coli O157:H7 on laboratory-inoculated alfalfa, radish, and mung bean seeds was compared with that of dry-heat treatment in combination with irradiation treatment. The treatment of mung bean seeds with EO water in combination with sonication followed by a rinse with sterile distilled water resulted in reductions of approximately 4.0 log10 CFU of E. coli O157:H7 per g. whereas reductions of ca. 1.52 and 2.64 log10 CFU/g were obtained for radish and alfalfa seeds. The maximum reduction (3.70 log10 CFU/g) for mung bean seeds was achieved by treatment with califresh-S and chlorinated water (200 ppm) in combination with sonication and a rinse. The combination of dry heat, hot EO water treatment, and sonication was able to eliminate pathogen populations on mung bean seeds but was unable to eliminate the pathogen on radish and alfalfa seeds. Other chemical treatments used were effective in greatly reducing pathogen populations on radish and alfalfa seeds without compromising the quality of the sprouts, but these treatments did not result in the elimination of pathogens from radish and alfalfa seeds. Moreover, a combination of dry-heat and irradiation treatments was effective in eliminating E. coli O157:H7 on laboratory-inoculated alfalfa, radish, and mung bean seeds. An irradiation dose of 2.0 kGy in combination with dry heat eliminated E. coli O157:H7 completely from alfalfa and mung bean seeds, whereas a 2.5-kGy dose of irradiation was required to eliminate the pathogen completely from radish seeds. Dry heat in combination with irradiation doses of up to 2.0 kGy did not unacceptably decrease the germination percentage for alfalfa seeds or the length of alfalfa sprouts but did decrease the lengths of radish and mung bean sprouts.

  9. Gene-Transformation-Induced Changes in Chemical Functional Group Features and Molecular Structure Conformation in Alfalfa Plants Co-Expressing Lc-bHLH and C1-MYB Transcriptive Flavanoid Regulatory Genes: Effects of Single-Gene and Two-Gene Insertion

    Directory of Open Access Journals (Sweden)

    Ravindra G. Heendeniya

    2017-03-01

    Full Text Available Alfalfa (Medicago sativa L. genotypes transformed with Lc-bHLH and Lc transcription genes were developed with the intention of stimulating proanthocyanidin synthesis in the aerial parts of the plant. To our knowledge, there are no studies on the effect of single-gene and two-gene transformation on chemical functional groups and molecular structure changes in these plants. The objective of this study was to use advanced molecular spectroscopy with multivariate chemometrics to determine chemical functional group intensity and molecular structure changes in alfalfa plants when co-expressing Lc-bHLH and C1-MYB transcriptive flavanoid regulatory genes in comparison with non-transgenic (NT and AC Grazeland (ACGL genotypes. The results showed that compared to NT genotype, the presence of double genes (Lc and C1 increased ratios of both the area and peak height of protein structural Amide I/II and the height ratio of α-helix to β-sheet. In carbohydrate-related spectral analysis, the double gene-transformed alfalfa genotypes exhibited lower peak heights at 1370, 1240, 1153, and 1020 cm−1 compared to the NT genotype. Furthermore, the effect of double gene transformation on carbohydrate molecular structure was clearly revealed in the principal component analysis of the spectra. In conclusion, single or double transformation of Lc and C1 genes resulted in changing functional groups and molecular structure related to proteins and carbohydrates compared to the NT alfalfa genotype. The current study provided molecular structural information on the transgenic alfalfa plants and provided an insight into the impact of transgenes on protein and carbohydrate properties and their molecular structure’s changes.

  10. Effect of absorbed dose and storage length on electron paramagnetic resonance (EPR) signal strength in irradiated alfalfa seeds

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    A kind of alfalfa seeds was irradiated by 1, 2, 3, 4 and 5 kGy at a dose rate of 6.288 kGy. h-1 in a self-shielded irradiator of 137Cs gamma rays. The EPR spectra, which were measured subsequently between 0.3401and 0.3501 T, showed that there was a direct proportional relationship between the EPR signal strength of free radicals produced by gamma irradiation in the alfalfa seeds and absorbed dose. The first derivative EPR spectra of the alfalfa seeds were very clear and easy to identify. However, the EPR signal strength of the peak-to-peak amplitude decreased rapidly and most of them decayed beyond 50% within 3 days after the seeds were irradiated. It tended to stabilize after half a month since the seeds were irradiated. The differences of the EPR signal strength between the irradiated and unirradiated alfalfa seeds still remained. All seeds were stored at ambient temperature for more than 3months. Therefore, using EPR spectrometry technique to measure free radicals in alfalfa seeds as a means to determine whether the seeds have been irradiated or not is feasible, relatively fast and simple.

  11. Identification of molecular markers associated with Verticillium wilt resistance in alfalfa (Medicago sativa L. using high-resolution melting.

    Directory of Open Access Journals (Sweden)

    Tiejun Zhang

    Full Text Available Verticillium wilt, caused by the soilborne fungus, Verticillium alfalfae, is one of the most serious diseases of alfalfa (Medicago sativa L. worldwide. To identify loci associated with resistance to Verticillium wilt, a bulk segregant analysis was conducted in susceptible or resistant pools constructed from 13 synthetic alfalfa populations, followed by association mapping in two F1 populations consisted of 352 individuals. Simple sequence repeat (SSR and single nucleotide polymorphism (SNP markers were used for genotyping. Phenotyping was done by manual inoculation of the pathogen to replicated cloned plants of each individual and disease severity was scored using a standard scale. Marker-trait association was analyzed by TASSEL. Seventeen SNP markers significantly associated with Verticillium wilt resistance were identified and they were located on chromosomes 1, 2, 4, 7 and 8. SNP markers identified on chromosomes 2, 4 and 7 co-locate with regions of Verticillium wilt resistance loci reported in M. truncatula. Additional markers identified on chromosomes 1 and 8 located the regions where no Verticillium resistance locus has been reported. This study highlights the value of SNP genotyping by high resolution melting to identify the disease resistance loci in tetraploid alfalfa. With further validation, the markers identified in this study could be used for improving resistance to Verticillium wilt in alfalfa breeding programs.

  12. Identification of molecular markers associated with Verticillium wilt resistance in alfalfa (Medicago sativa L.) using high-resolution melting.

    Science.gov (United States)

    Zhang, Tiejun; Yu, Long-Xi; McCord, Per; Miller, David; Bhamidimarri, Suresh; Johnson, David; Monteros, Maria J; Ho, Julie; Reisen, Peter; Samac, Deborah A

    2014-01-01

    Verticillium wilt, caused by the soilborne fungus, Verticillium alfalfae, is one of the most serious diseases of alfalfa (Medicago sativa L.) worldwide. To identify loci associated with resistance to Verticillium wilt, a bulk segregant analysis was conducted in susceptible or resistant pools constructed from 13 synthetic alfalfa populations, followed by association mapping in two F1 populations consisted of 352 individuals. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were used for genotyping. Phenotyping was done by manual inoculation of the pathogen to replicated cloned plants of each individual and disease severity was scored using a standard scale. Marker-trait association was analyzed by TASSEL. Seventeen SNP markers significantly associated with Verticillium wilt resistance were identified and they were located on chromosomes 1, 2, 4, 7 and 8. SNP markers identified on chromosomes 2, 4 and 7 co-locate with regions of Verticillium wilt resistance loci reported in M. truncatula. Additional markers identified on chromosomes 1 and 8 located the regions where no Verticillium resistance locus has been reported. This study highlights the value of SNP genotyping by high resolution melting to identify the disease resistance loci in tetraploid alfalfa. With further validation, the markers identified in this study could be used for improving resistance to Verticillium wilt in alfalfa breeding programs.

  13. Trend,lessons and enlightenment of the alfalfa trade in the United States%美国苜蓿贸易--趋势、经验与启示

    Institute of Scientific and Technical Information of China (English)

    汪武静; 王明利; 吕官旺; 刘玉凤; 石自忠

    2016-01-01

    随着我国奶产业及其它草食畜牧业转型和升级步伐的加快,市场对优质牧草的需求快速增加,特别是从美国进口苜蓿(Medicago sativa )呈“井喷式”增长态势。我国苜蓿作为一个产业发展只是近年来才起步。尽管国内苜蓿生产满足不了强大的市场需求,但研究其国际市场贸易,可以有效借鉴国际经验,尽快赶上国际苜蓿贸易发展水平。美国是苜蓿生产和贸易大国,以美国为研究对象,分析其苜蓿贸易趋势和经验,并提出相关启示对我国苜蓿贸易具有重要意义。研究结果表明,美国苜蓿愈加受到各国青睐,出口量不断增加,出口价格不断上涨;高标准、严要求的苜蓿产品质量是保障出口强有力的前提,高水平的营销策略是推动出口的重要驱动力,强大的信息追溯体系是保障质量的重要手段,低成本的运输系统是美国苜蓿出口竞争力提升的重要条件。国内应重视苜蓿国际贸易市场,提高营销策略,完善二次压缩技术,降低交通运输成本,规范实体产业并建设草产品质量追溯体系,尽快提升我国苜蓿生产水平和质量档次,从而提升苜蓿产业的竞争力。%With the rapid transformation of Chinese dairy industry and other grass-feeding livestock industry, the demand of high-quality alfalfa imported from the United States has been increased fast.As a new industry developed in recent years,Chinese alfalfa industry could not meet the huge market demand.The study on the international market trade of the United States can be effectively useful to catch up with the development of in-ternational standards.The United States is the most powerful country of alfalfa production and trading,it is of great value to analyze the alfalfa trade trend and sum up the trade experience.The findings indicate that the ex-port quantity of alfalfa produced in the United States has been gradually increased

  14. Plant Variety Protection and DUS Testing Technique

    Institute of Scientific and Technical Information of China (English)

    LI Xiao-hui; LI Xin-hai; ZHANG Shi-huang; LI Ming-shun; LI Wen-hua

    2002-01-01

    DUS testing technique used for plant variety protection was reviewed in the paper, and somesuggestions were made on how to establish the appropriate technology system in China. Meanwhile, the poten-tial exploitation of the technique was discussed.

  15. Product variety and the demographic transition

    DEFF Research Database (Denmark)

    Guzmán, Ricardo Andrés; Weisdorf, Jacob Louis

    2010-01-01

    Why does the rate of population growth decline in the face of economic growth? We show that growing product variety may induce a permanent reduction in the demand for children and a continuous rise in income and consumption....

  16. Product Variety and the Demographic Transition

    DEFF Research Database (Denmark)

    Guzmán, Ricardo Andrés; Weisdorf, Jacob Louis

    Why does the rate of population growth decline in the face of economic growth? We show that growing product variety may induce a permanent reduction in the demand for children and a continuous rise in income and consumption....

  17. Product Variety and the Demographic Transition

    DEFF Research Database (Denmark)

    Guzmán, Ricardo Andrés; Weisdorf, Jacob Louis

    Why does the rate of population growth decline in the face of economic growth? We show that growing product variety may induce a permanent reduction in the demand for children and a continuous rise in income and consumption.......Why does the rate of population growth decline in the face of economic growth? We show that growing product variety may induce a permanent reduction in the demand for children and a continuous rise in income and consumption....

  18. Representations of fundamental groups of algebraic varieties

    CERN Document Server

    Zuo, Kang

    1999-01-01

    Using harmonic maps, non-linear PDE and techniques from algebraic geometry this book enables the reader to study the relation between fundamental groups and algebraic geometry invariants of algebraic varieties. The reader should have a basic knowledge of algebraic geometry and non-linear analysis. This book can form the basis for graduate level seminars in the area of topology of algebraic varieties. It also contains present new techniques for researchers working in this area.

  19. On Special Subvarieties of Kuga Varieties

    CERN Document Server

    Chen, Ke

    2011-01-01

    In this paper we prove the equidistribution of certain families of special subvarieties in Kuga varieties, which is a special case of the general Andre-Oort conjecture formulated for mixed Shimura varieties proposed by R.Pink. Our approach is parallel to the pure case treated in the works of L.Clozel, E.Ullmo, and A.Yafaev using tools from ergodic theory.

  20. Identification of Carnation varieties using microsatellite markers

    OpenAIRE

    Arens, P.F.P.; Esselink, G.; Noordijk, Y.; Kodde, L.P.; Hof, L.; Wietsma, W.A.; Vosman, B.

    2009-01-01

    As in many ornamentals, also in carnation the number of varieties in common knowledge is large and identification throughout the chain from breeder to consumer using plant material from different stages and organs may be needed. Results in this study on the use of microsatellite markers from Dianthus caryophyllus L. for the characterization of carnation varieties as well as the construction and evaluation of a molecular database show that these markers show potential for identification purposes

  1. Cohomological Hall algebras and character varieties

    CERN Document Server

    Davison, Ben

    2015-01-01

    In this paper we investigate the relationship between twisted and untwisted character varieties via a specific instance of the Cohomological Hall algebra for moduli of objects in 3-Calabi-Yau categories introduced by Kontsevich and Soibelman. In terms of Donaldson--Thomas theory, this relationship is completely understood via the calculations of Hausel and Villegas of the E polynomials of twisted character varieties and untwisted character stacks. We present a conjectural lift of this relationship to the cohomological Hall algebra setting.

  2. Meiotic behavior of several Brazilian soybean varieties

    OpenAIRE

    Bione Nilton Cesar Pires; Pagliarini Maria Suely; Toledo José Francisco Ferraz de

    2000-01-01

    Despite the importance of soybeans little cytogenetic work has traditionally been done, due to the small size and apparent similarity of the chromosomes. Fifteen soybean [Glycine max (L.) Merrill] varieties adapted for cultivation in two distinct regions of Brazil were analyzed cytogenetically. A low frequency of meiotic abnormalities was noted in all varieties, although they were not equally affected. Irregular chromosome segregation, chromosome stickiness, cytoplasmic connections between ce...

  3. Soluble phenolic compounds in different cultivars of red clover and alfalfa, and their implication for protection against proteolysis and ammonia production in ruminants

    Science.gov (United States)

    Red clover contains phenolic compounds with roles in inhibiting proteolysis and loss of amino acids as ammonia. Alfalfa has been found to have lower concentrations of phenolic compounds, but few alfalfa and red clover cultivars have been compared for phenolic content. Total soluble phenolic compou...

  4. Isozyme Analysis on Different Varieties of Sugarcane

    Directory of Open Access Journals (Sweden)

    Johnson M.

    2012-05-01

    Full Text Available Isozymic and protein diversity among five sugarcane varieties viz., Co 6304, Co 85019, Co 8371, Co 89003 and Co 91010 were studied to understand the varietal interrelationship and to identify the biochemical marker for the disease resistance and stress tolerance. The standard technique of vertical gel electrophoresis PAGE was employed for size separation of isozymes. The gel was stained with different staining solutions for different isozyme systems viz. peroxidase, esterase, acid phosphatase, alkaline phosphatase and proteins. Rf values of the banding profiles, similarity index and variation between the varieties were analysed. Among the four enzyme systems, peroxidase profile reveals the difference between the disease resistant / susceptible and abiotic stress tolerant / non tolerant varieties. The two isoperoxidase bands with Rf values 0.62 and 0.66 showed their presence in disease resistant and abiotic tolerant varieties. The presence of two marker bands (0.62, 0.66 of resistant and stress tolerant varieties suggest that the variety Co 6304 may also be resistant to smut, wilt and moderately resistant to red rot and tolerant to drought.

  5. Determination of physicochemical characteristics in different products of tomato varieties.

    Science.gov (United States)

    Akbudak, Bulent; Bolkan, Hasan; Cohen, Nancy

    2009-01-01

    The objective of this work was to study the newly developed and existing tomato (Lycopersicon esculentum Mill.) varieties for physicochemical changes in the fruits in the ripe period. Varieties 'CXD179' and 'CX8401' in fresh and canned, and 'CXD203' and 'CXD276' in paste were found superior with respect to ascorbic acid and antioxidant capacity. The highest lycopene and beta-carotene contents were determined in 'CXD203', 'CXD276', and 'CX8401'. Color values showed a change from a greenness value of 'a' to redness values, whereas 'b' decreased. In general, parameter values were higher in canned samples. Studies showed that the lycopene content best correlated with 'a' values in the ripening. Inter-variety variation in physicochemical parameters at the ripe stage revealed that 'CXD179' and 'CXD203' in all products among red varieties were the best varieties. Among light color varieties 'CXD276' and 'CX8401' in view of red color, and 'CX8400' and 'CX8402' in view of green color were better.

  6. Experimental Precision and Variety Comparison Precision in Regional Crop Trials

    Institute of Scientific and Technical Information of China (English)

    KONG Fan-ling; ZHANG Qun-yuan; GE Zhi-nan; YANG Fu-xin; ZHANG De-gui; LIU Wen-xin

    2002-01-01

    According to the basic concepts of precision and the principles of analysis of variance (ANOVA), precision types for experiments and variety comparison in regional crop trials (RCT) were studied and developed; expected variety comparison precision (EVCP) and realized variety comparison precision (RVCP) and the corresponding statistical indexes of them were proposed. It was explained that experimental precision (EP) and variety comparison precision (VCP) are two kinds of precision of RCT; EP includes error precision and variety mean precision, which can be measured respectively by the coefficient of variation of single observation's error (CVe) and the coefficient of variation of variety mean's error (CVY); VCP includes EVCP and RVCP, which can be measured respectively by the detectable least relative difference (DLRD) and the relative least significant distance (RLSD); EP is an important factor of VCP but not identical to it; RVCP is the realization of EVCP. Besides error, experimental design and GE interaction and ANOVA model affect VCP. Several application examples for these precision indexes were presented, and the precision of regional cotton trials in the Yellow River Valley and the Changjiang Valley were investigated through the historical data of RCTs from 1980 to 1999.

  7. 75 FR 77612 - Plant Variety Protection Board; Reestablishment of the Plant Variety Protection Board

    Science.gov (United States)

    2010-12-13

    ... Agricultural Marketing Service Plant Variety Protection Board; Reestablishment of the Plant Variety Protection... Agriculture intends to reestablish the Plant Variety Protection Board. FOR FURTHER INFORMATION CONTACT: Paul....regulations.gov or by e-mail: Paul.Zankowski@ams.usda.gov . SUPPLEMENTARY INFORMATION: The Plant...

  8. Related Variety, Unrelated Variety and Technological Breakthroughs: An analysis of US State-Level Patenting

    NARCIS (Netherlands)

    Castaldi, C.; Frenken, K.; Los, B.

    2015-01-01

    Castaldi C., Frenken K. and Los B. Related variety, unrelated variety and technological breakthroughs: an analysis of US state-level patenting, Regional Studies. This paper investigates how variety affects the innovation output of a region. Borrowing arguments from theories of recombinant innovation

  9. Chemical Composition of Different Varieties of Linseed

    Directory of Open Access Journals (Sweden)

    M. Laiq Khan*, M. Sharif, M. Sarwar, Sameea1 and M. Ameen

    2010-04-01

    Full Text Available The present study was conducted to investigate chemical composition of six varieties of linseed (Chandni, LS-29, LS-49, LS-70, LS-75 and LS-76. Proximate composition, mineral profile and cyanogenic glycosides (linamarin were determined. Average proximate composition values for linseed i.e. crude protein, ether extract, crude fiber, ash and nitrogen free extract were 24.18, 37.77, 4.78, 3.50 and 25.86%, respectively. Higher values of crude protein, ether extract, crude fiber and nitrogen free extract were observed in varieties LS-49, LS-70, LS-29 and Chandni, respectively. Average mineral contents in linseed i.e. Ca, Mg, K, Na, Cl, P, Cu, Fe, Mn and Zn were 0.39, 0.09, 1.41, 0.05, 0.08, 0.89, 4.67, 50.56, 8.29 and 13.55 ppm, respectively. Among micro minerals, varieties LS-29 and LS-70 were higher in Cu contents; LS-75 was higher in Fe content, while LS-49 was higher in Mn and Zn contents. Among macro minerals, level of Ca was higher in LS-70, levels of Mg, K and Na were higher in Chandni, while P was higher in LS-49. Average amount of linamarin in linseed was 31.05mg/100 gm DM. The variety LS-75 had the highest (35.22 mg/100 gm linamarin content, while variety LS-70 had least (26.22 mg/100 gm amount of linamarin. In conclusion, there is significant difference in chemical composition among linseed varieties. The varieties LS-49 showed higher crude protein content, LS-70 showed greater oil content, while LS-75 had higher content of linamarin.

  10. Supplemental cracked corn for steers fed fresh alfalfa: I. Effects on digestion of organic matter, fiber, and starch.

    Science.gov (United States)

    Elizalde, J C; Merchen, N R; Faulkner, D B

    1999-02-01

    The effect of supplementation with different levels of cracked corn on the sites of OM, total dietary fiber (TDF), ADF, and starch digestion in steers fed fresh alfalfa indoors was determined. Six Angus steers (338 +/- 19 kg) fitted with cannulas in the rumen, duodenum, and ileum consumed 1) alfalfa (20.4% CP, 41.6% NDF) ad libitum (AALF); 2), 3), and 4) AALF supplemented (S) with .4, .8, or 1.2%, respectively, of BW of corn; or 5) alfalfa restricted at the average level of forage intake of S steers (RALF), in a 5 x 5 Latin square design. Total OM intake was lower (P .05). Forage OM intake decreased (P fiber digestion was not affected.

  11. Environmental life cycle assessment of producing willow, alfalfa and straw from spring barley as feedstocks for bioenergy or biorefinery systems

    DEFF Research Database (Denmark)

    Parajuli, Ranjan; Knudsen, Marie Trydeman; Djomo, Sylvestre Njakou

    2017-01-01

    and land occupation. Environmental impacts for straw were economically allocated from the impacts obtained for spring barley. The results obtained per ton dry matter showed a lower carbon footprint for willow and alfalfa compared to straw. It was due to higher soil carbon sequestration and lower N2O...... output to input ratio, willow performed better than other biomasses. On the basis of carbohydrate content of straw, the equivalent dry matter of alfalfa and willow would be requiring higher. The environmental impacts of the selected biomasses in biorefinery therefore would differ based on the conversion......The current study aimed at evaluating potential environmental impacts for the production of willow, alfalfa and straw from spring barley as feedstocks for bioenergy or biorefinery systems. A method of Life Cycle Assessment was used to evaluate based on the following impact categories: Global...

  12. Overexpression of GsZFP1 enhances salt and drought tolerance in transgenic alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Tang, Lili; Cai, Hua; Ji, Wei; Luo, Xiao; Wang, Zhenyu; Wu, Jing; Wang, Xuedong; Cui, Lin; Wang, Yang; Zhu, Yanming; Bai, Xi

    2013-10-01

    GsZFP1 encodes a Cys2/His2-type zinc-finger protein. In our previous study, when GsZFP1 was heterologously expressed in Arabidopsis, the transgenic Arabidopsis plants exhibited enhanced drought and cold tolerance. However, it is still unknown whether GsZFP1 is also involved in salt stress. GsZFP1 is from the wild legume Glycine soja. Therefore, the aims of this study were to further elucidate the functions of the GsZFP1 gene under salt and drought stress in the forage legume alfalfa and to investigate its biochemical and physiological functions under these stress conditions. Our data showed that overexpression of GsZFP1 in alfalfa resulted in enhanced salt tolerance. Under high salinity stress, greater relative membrane permeability and malondialdehyde (MDA) content were observed and more free proline and soluble sugars accumulated in transgenic alfalfa than in the wild-type (WT) plants; in addition, the transgenic lines accumulated less Na(+) and more K(+) in both the shoots and roots. Overexpression of GsZFP1 also enhanced the drought tolerance of alfalfa. The fold-inductions of stress-responsive marker gene expression, including MtCOR47, MtRAB18, MtP5CS, and MtRD2, were greater in transgenic alfalfa than those of WT under drought stress conditions. In conclusion, the transgenic alfalfa plants generated in this study could be used for farming in salt-affected as well as arid and semi-arid areas.

  13. Increased photosynthetic acclimation in alfalfa associated with arbuscular mycorrhizal fungi (AMF) and cultivated in greenhouse under elevated CO2.

    Science.gov (United States)

    Goicoechea, Nieves; Baslam, Marouane; Erice, Gorka; Irigoyen, Juan José

    2014-11-15

    Medicago sativa L. (alfalfa) can exhibit photosynthetic down-regulation when grown in greenhouse conditions under elevated atmospheric CO2. This forage legume can establish a double symbiosis with nitrogen fixing bacteria and arbuscular mycorrhizal fungi (AMF), which may increase the carbon sink effect of roots. Our aim was to assess whether the association of alfalfa with AMF can avoid, diminish or delay the photosynthetic acclimation observed in previous studies performed with nodulated plants. The results, however, showed that mycorrhizal (M) alfalfa at the end of their vegetative period had lower carbon (C) discrimination than non-mycorrhizal (NM) controls, indicating photosynthetic acclimation under ECO2 in plants associated with AMF. Decreased C discrimination was due to the acclimation of conductance, since the amount of Rubisco and the expression of genes codifying both large and small subunits of Rubisco were similar or slightly higher in M than in NM plants. Moreover, M alfalfa accumulated a greater amount of soluble sugars in leaves than NM plants, thus favoring a down-regulation effect on photosynthetic rates. The enhanced contents of sugars in leaves coincided with a reduced percentage of arbuscules in roots, suggesting decreased sink of carbohydrates from shoots to roots in M plants. The shorter life cycle of alfalfa associated with AMF in comparison with the NM controls may also be related to the accelerated photosynthetic acclimation in M plants. Further research is needed to clarify to what extent this behavior could be extrapolated to alfalfa cultivated in the field and subjected to periodic cutting of shoots under climatic change scenarios.

  14. Extending ALFALFA in the Direction of the Pisces-Perseus Supercluster with the Arecibo L-Band Wide Receiver

    Science.gov (United States)

    O'Donoghue, Aileen A.; Haynes, Martha P.; Koopmann, Rebecca A.; Jones, Michael G.; Hallenbeck, Gregory L.; Giovanelli, Riccardo; Hoffman, Lyle; Craig, David W.; Undergraduate ALFALFA Team

    2017-01-01

    We have completed three “Harvesting ALFALFA” Arecibo observing programs in the direction of the Pisces-Perseus Supercluster (PPS) since ALFALFA observations were finished in 2012. The first was to perform follow-up observations on high signal-to-noise (S/N > 6.5) ALFALFA detections needing confirmation and low S/N sources lacking optical counterparts. A few more high S/N objects were observed in the second program along with targets visually selected from the Sloan Digital Sky Survey (SDSS). The third program included low S/N ALFALFA sources having optical counterparts with redshifts that were unknown or differed from the ALFALFA observations. It also included more galaxies selected from SDSS by eye and by Structured Query Language (SQL) searches with parameters intended to select galaxies at the distance of the PPS (~6,000 km/s). We used pointed basic Total-Power Position-Switched Observations in the 1340 - 1430 MHz ALFALFA frequency range. For sources of known redshift, we used the Wideband Arecibo Pulsar Processors (WAPP’s) , while for sources of unknown redshift we utilized a hybrid/dual bandwidth Doppler tracking mode using the Arecibo Interim 50-MHz Correlator with 9-level sampling.Results confirmed that a few high S/N ALFALFA sources are spurious as expected from the work of Saintonge (2007), low S/N ALFALA sources lacking an optical counterpart are all likely to be spurious, but low S/N sources with optical counterparts are generally reliable. Of the optically selected sources, about 80% were detected and tended to be near the distance of the PPS.This work has been supported by NSF grant AST-1211005.

  15. Effect of Graded Levels of Fiber from Alfalfa Meal on Nutrient Digestibility and Flow of Fattening Pigs

    Institute of Scientific and Technical Information of China (English)

    CHEN Liang; GAO Li-xiang; ZHANG Hong-fu

    2014-01-01

    The ifber level and composition have an important effect on nutrient digestibility of swine diets. Little information is known about the effects of ifber level and composition from alfalfa meal on nutrient digestibility of fattening pigs fed a corn-soybean meal-based diet. The objective of this experiment was to determine the effects of alfalfa ifber on the growth performance, intestinal nutrient lfow and apparent total tract digestibility (ATTD) of nutrients in fattening pigs. 24 barrows (Duroc×(Large White×Landrace), body weight=(60.6±0.7) kg) were randomly allotted to 4 treatments with 6 replicates of 1 pig per replicate. The pigs were provided a control diet or a diet containing 5, 10 or 20%of alfalfa meal during a 14-d experiment period. Average daily gain (ADG) and the ATTD of dry matter (DM), organic matter (OM), crude protein (CP), neutral detergent ifber (NDF), acid detergent ifber (ADF) and gross energy (GE) reduced linearly as the level of alfalfa meal in the diet increased (P0.05). A multiple linear regression analysis, taking into account both soluble and insoluble ifber intake, explained approximately 70%of the variation in the ATTD of DM, OM, NDF, and GE (P<0.01). In conclusion, alfalfa meal should be limited to less than 5%of the diet in fattening pigs to maximize growth performance and nutrient digestion. Soluble and insoluble ifber from alfalfa meal has the differential roles in nutrient digestion, which may help explain the main variation observed in nutrient digestibility. These ifndings suggest that knowledge of speciifc ifber components is necessary to accurately predict the effects of dietary ifber on nutrient digestibility.

  16. Effects of ozone and water stress on canopy temperature, water use, and water use efficiency of alfalfa

    Energy Technology Data Exchange (ETDEWEB)

    Temple, P.J. (Univ. of California, Riverside (USA)); Benoit, L.F. (Univ. of California, Davis (USA))

    Ozone (O{sub 3}) and soil water deficit are two environmental stresses that significantly affect the growth and yield of alfalfa (Medicago sativa L). However, little is known of the responses of field-grown alfalfa to O{sub 3}, and the effects of the interaction between O{sub 3} and water stress on canopy temperature and water relations of alfalfa have not been previously reported. The objective of this 2-yr study was to determine the interactive effects of O{sub 3} and soil water deficits on canopy temperatures, water use, and water use efficiency (WUE) of alfalfa. Alfalfa (cv. WL-514) was grown in 30-3- by 5.5-m plots on Wasco sandy loam (coarse-loamy, mixed, nonacid, thermic Typic Torriorthents) in Shafter, CA, and was exposed in open-top chambers to five levels of O{sub 3} for 12 h daily, from March to October of 1984 and 1985. Ozone treatments ranged from charcoal-filtered air (CF) to twice ambient O{sub 3} concentrations. Each plot received either normal amounts of irrigation (NI) or 30% less than normal (WS). Canopy temperature-air temperature differentials ({Tc}-T{sub a}) were significantly reduced by water stress an average of 27.9% in 1984 and 44.0% in 1985. Ozone also significantly reduced {Tc}-T{sub a} by 31% in NI and 37% in WS plots in 1984, but in 1985 O{sub 3} had no effect on {Tc}-T{sub a}. Water use, rate of soil water depletion, or depth of effective rooting zone were not affected by O{sub 3}, whereas water stress significantly reduced all three. Water use efficiency was significantly reduced by O{sub 3}, averaging 12% lower in nonfiltered compared with CF plots. The effects of O{sub 3} on WUE were attributed to premature senescence and abscission of older alfalfa leaves.

  17. Cloning and characterization of chromosomal markers in alfalfa (Medicago sativa L.).

    Science.gov (United States)

    Yu, Feng; Lei, Yunting; Li, Yuan; Dou, Quanwen; Wang, Haiqing; Chen, Zhiguo

    2013-07-01

    Eleven tandemly repetitive sequences were identified from a Cot-1 library by FISH and sequence analysis of alfalfa (Medicago sativa). Five repetitive sequences (MsCR-1, MsCR-2, MsCR-3, MsCR-4, and MsCR-5) were centromeric or pericentromeric, of which three were satellite DNAs and two were minisatellite DNAs. Monomers of 144, 148, and 168 bp were identified in MsCR-1, MsCR-2, and MsCR-3, respectively, while 15 and 39 bp monomers were identified in MsCR-4 and MsCR-5, respectively. Three repetitive sequences were characterized as subtelomeric; one repetitive sequence, MsTR-1, had a 184 bp monomer, and two repetitive sequences had fragments of 204 and 327 bp. Sequence analysis revealed homology (70-80 %) between MsTR-1 and a highly repeated sequence (C300) isolated from M. ssp. caerulea. Three identified repetitive sequences produced hybridization signals at multiple sites in a few of the chromosomes; one repetitive sequence was identified as the E180 satellite DNA previously isolated from M. sativa, while the other 163 and 227 bp fragments had distinct sequences. Physical mapping of the repetitive sequences with double-target FISH revealed different patterns. Thus, nine novel tandemly repetitive sequences that can be adopted as distinct chromosome markers in alfalfa were identified in this study. Furthermore, the chromosome distribution of each sequence was well described. Though significant chromosome variations were detected within and between cultivars, a molecular karyotype of alfalfa was suggested with the chromosome markers we identified. Therefore, these novel chromosome markers will still be a powerful tool for genome composition analysis, phylogenetic studies, and breeding applications.

  18. Activity screening of plant growth promoting rhizobacteria isolated from alfalfa rhizosphere

    Directory of Open Access Journals (Sweden)

    shahla pashapour

    2016-03-01

    Full Text Available Introduction: Some rhizobacteria by various mechanisms influence plant growth as they are called plant growth promoting rhizobacteria (PGPR. Scientists identified some PGPR characters involved in promoting plant growth, while all these characters are not able to study. The aim of this study was to evaluate PGP activities of bacterial isolates, (45 isolates belonged to rhizobium and 2 bacterial isolates belonged to Pseudomonas fluorescens, which were isolated from alfalfa (Medicago sativa rhizosphere and root nodules grown around Zanjan. Materials and methods: These bacteria were isolated from alfalfa roots grown around Zinc industries in Zanjan province. After bacterial isolation and purification from root and soil samples, isolates were screened in vitro for plant growth promoting traits such as IAA (Indole Acetic Acid, ACC- deaminase (Amino Cyclopropan Carboxylate, HCN (Hydrogen Cyanide, siderophore, chitinase production and mineral and organic phosphate solubilization activities. Results: The results indicated that 43 bacterial isolates produced IAA (4.04- 4.95 μg/ml and 15 isolates produced ACC- deaminase (0.23- 1.05 μg/ml. Only one isolate (Rm66 produced high amount of HCN. Qualitative siderophore production was observed in 9 isolates. None of the isolates produced chitinase. Solubilization of mineral phosphate was commonly detected in 19 isolates (4.33- 5.86 μg/ml, and 15 isolates solubilized organic phosphate (1.66- 144.28 μg/ml. Discussion and conclusion: This study shows that most of the bacterial strains which isolated from alfalfa cultivated lands had PGP activities and also a good potential to increase plant growth after inoculation with to seeds as eco- friendly fertilizers.

  19. Acidified sodium chlorite as an alternative to chlorine for elimination of salmonella on alfalfa seeds.

    Science.gov (United States)

    Liao, C-H

    2009-01-01

    The health and environmental hazard associated with the use of chlorine for food processing has been documented previously. This study was conducted to determine if acidified sodium chlorite (ASC) could be used to replace calcium hypochlorite (Ca[OCl](2)) for disinfection of alfalfa seeds. Contaminated seeds containing approximately 1.5 x 10(7) CFU/g of Salmonella were treated with ASC or Ca(OCl)(2) at different concentrations and for different periods of time. Results showed that the efficacy of ASC and Ca(OCl)(2) for elimination of Salmonella on contaminated seeds could be improved greatly by extending the treatment time from the traditional 15 to 45 min. Treatment of seeds with 800 ppm of ASC for 45 min reduced the number of Salmonella by 3.9 log units, approximately 1.2 log units higher than that treated with 20000 ppm of Ca(OCl)(2). Treatment of seeds with a lower concentration (100 to 400 ppm) of ASC for 45 min reduced the number of Salmonella by 1.3 to 2.2 log units. Soaking alfalfa seeds in 800 ppm of ASC for 45 min did not affect seed germination. However, soaking seeds in 20000 ppm of Ca(OCl)(2) for 45 min reduced seed germination by 20%. Unlike Ca(OCl)(2), antimicrobial efficiency of ASC was not affected by pre-exposure to alfalfa seeds. Data presented also showed that Salmonella on newly inoculated seeds that had been stored at 4 degrees C for less than 7 d were more sensitive to sanitizer treatment than those on seeds that had been stored for 4 wk or longer.

  20. Compression of fractionated sun-cured and dehydrated alfalfa chops into cubes--specific energy models.

    Science.gov (United States)

    Adapa, Phani; Schoenau, Greg; Tabil, Lope; Sokhansanj, Shahab; Singh, Asheesh

    2007-01-01

    The objective of this study was to determine the specific energy requirements for the compression of fractionated sun-cured and dehydrated alfalfa chops, when subjected to different pressures and holding times. The compression behavior of fractionated sun-cured and dehydrated alfalfa chops was studied using a single cubing unit capable of making one cube in a single stroke of the plunger. The cube die dimensions were 30 mm x 30 mm in cross-section and an effective depth of compression of 0.38 m. The initial moisture content of dehydrated and sun-cured chops were 6% and 7% (wb), respectively. A stack of two sieves (instead of five) was used along with a pan to achieve leaf and stem separation. The nominal opening sizes of two sieves with square holes were 3.96 and 1.17 mm, respectively. Leaf and stem fractions were combined later to obtain five different samples each for sun-cured and dehydrated alfalfa with leaf content ranging from 0% to 100% by mass in increments of 25%. The chop moisture content and preheat temperature before compaction was 10% (wb) and 75 degrees C, respectively. The cube die temperature was maintained at 90+/-5 degrees C. The mass of chops used for making each cube was 23+/-02 g. A hydraulic press was used to apply 9.0, 12.0 and 14.0 MPa of pressures through a plunger. After compression, the plunger was held in place for 10 and 30s, before the compacted forage was extracted. Empirical equations were fitted to the data relating specific energy for cube making to pressure, residence time, and leaf content.